LOCUS       DAORVQ010000001        65783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_106_length_65783_cov_6.864791, whole genome shotgun sequence.
ACCESSION   DAORVQ010000001 DAORVQ010000000
VERSION     DAORVQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 65783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65783
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106_length_65783_cov_6.864791"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1695)
                     /locus_tag="PLX80_00005"
     CDS             complement(244..1695)
                     /locus_tag="PLX80_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY19205.1"
                     /translation="MTEKFSKIGFGCYRISDKIREHYDSLYKALTSGVNLIDTSSNYS
                     VGGSEVLVGKVVNDLINEGKLKREDLCIVTKGGYIQGILLAELKQKKENGIYFEEVVE
                     ESDRLWHCISPDFIEDQLKRQFERLNTPYIDYYLLHNPEYYLDHAASNNPDLESSREE
                     YYKRIERAFRFLEEKCDSGEIKFYGISSNTFPSYSDERSFTSLEKVYEIANSISENNR
                     FKMIQLPLNLFEAGAVRNKNQENKTKTVLEFAAKKELKVLINRPLNAITSKGLVRLAE
                     FKADAFEEKDFIKQIKLVKLLEEDMLSEKLSEDEFGEEEYKKLKNNLITGNQIEENWK
                     FFGSIEHFNDLIRQLFAPRINILLDFFEKNISDEGVKEFSNHYIQNVYLLLKFTGNYY
                     KLRADKRSRFINKLINEKLSPEYHSLTLSQKAALILISVKGVDCVLAGMTKPEYTDDL
                     LKVMSVEKICNADEIIMYVSLKIETEENVMEIS"
     gene            2162..3028
                     /locus_tag="PLX80_00010"
     CDS             2162..3028
                     /locus_tag="PLX80_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19206.1"
                     /translation="MKDFDKLWDYSDPAGTEIKFREILDEVKNSNDNSAYLQLQTQIA
                     RTLGLQMKFDDAHKLLDEVEKELSDKEDPVARVRYLLERGRAFNSSKQKDKARELFLK
                     AYELAKKEGEDNFAVDAAHMMGIVEPGDESLRWNEIAMKDAEASDVPKARSWLGSLYN
                     NTGWTYFDMKDYDKALAVFTKCKNWYEEKERPLEIAIAKWSIAKTLRMMGKTDEALEI
                     QLALLKDIEDGKAGSDGYIYEELTECYLLKNEKTKASDFAGKAYELLSKDIWVAENEK
                     EKLERLKNISIG"
     gene            3033..3383
                     /locus_tag="PLX80_00015"
     CDS             3033..3383
                     /locus_tag="PLX80_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19207.1"
                     /translation="MKIGHIEIFVKDPMVSKDFYEKILGFTVTEVQHEKYVWLKLGKT
                     QILLRPGKNDLKTNEYKDSKVGIVIYTDDHSKTRSELEGKGLVFCGTDGSDSCLTFKD
                     PDGNWFQLVNPSHA"
     gene            3413..4006
                     /locus_tag="PLX80_00020"
     CDS             3413..4006
                     /locus_tag="PLX80_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HQY19208.1"
                     /translation="MSSKLSPEEIKKTEEQLGELDKELELLRKKRIELNSKLAQMEVS
                     DYTMKDRNGNEKKLSEMFGDKSELILIHNMGKSCSYCTMWADGFKDTYKEIEKKAAFV
                     LVCPDKPEVHKEFAEAHGWNYPTYSANGTEFIYDMGYEIRKEDKTYYWPGVSVFEKKD
                     DGSILRVSKDYFGPGDFYCNIWHFLDLLPSEDITTDS"
     gene            4107..4601
                     /locus_tag="PLX80_00025"
     CDS             4107..4601
                     /locus_tag="PLX80_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19209.1"
                     /translation="MSRTVTDVFAKQYDVMVEWVNGVISGLADEDFNLELAPGKNNGV
                     WLLGHLIVCDDDFSEYMGKGDILYPQYIELFGQHTKVQTPDKYPSVKELRESWEKVCE
                     KNVNVYKQITDEELKEDHALVKNAEDDYFKTKENVIMHWQLHEMYHAGQLAVILSICG
                     KSKY"
     gene            4617..5264
                     /locus_tag="PLX80_00030"
     CDS             4617..5264
                     /locus_tag="PLX80_00030"
                     /inference="COORDINATES: protein motif:HMM:NF025718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HQY19210.1"
                     /translation="MTKFISAWKQYLSYPHRKAEFLITILLLIITLTTFSRFLLFNEA
                     RQGAILDDPILSLFNAIDLNALLFFAIYSSLLIALFFFSFDPEVLLTGIQTYILLVMF
                     RMSAMYLIPLDPPVGCIDLQDPVVFVMGTGKKIVKDLFFSGHTSTAFLLFQTAKNKYL
                     KTYFLLATLTVGIAVILQKAHYTIDVYAGLIFSFASYQIVKKIRAKFPLVKNKSA"
     gene            5264..5653
                     /locus_tag="PLX80_00035"
     CDS             5264..5653
                     /locus_tag="PLX80_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY19211.1"
                     /translation="MKERKKFSSGVIWEDIVGYSRAVKIDDRILVSGTTAIVDGKIVG
                     KDDMYLQTRTIIEKIESVLIQAGSSLEDIIRLRIFVTDITKWEEAGKALGEKFRTIKP
                     AQTLVGISALVDPDMLVEIEAEAITTS"
     gene            5732..6640
                     /locus_tag="PLX80_00040"
     CDS             5732..6640
                     /locus_tag="PLX80_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY19212.1"
                     /translation="MDNPQTESKTNLLSIFKGYLLALWGILFLMIVFFFFGDKILSES
                     YYLNTSVFLTNTFDTNFFVYCLVGFFAQVIDGSLGMAYGVSSTSFLLSTGVSPAVASA
                     SVHVAEVFTTGASGFSHWKFGNIDKKLFLKLLLPGSIGAAIGAFLLTSIDGNDIKPFV
                     SIYLGVMGIIIIIKAFKKVILFKDHGRVSLLALIGGFVDNIGGGGWGPVVTTTLISSG
                     GIPRYTVGSVNAAEFFVSLTGATVFTITIGIDHWNIIAGLIAGGLIAAPVGAFLCSRI
                     NPKILMIIVGVLIVFLSIRTVFLSVI"
     gene            6835..7350
                     /locus_tag="PLX80_00045"
     CDS             6835..7350
                     /locus_tag="PLX80_00045"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HQY19213.1"
                     /translation="MLPPINFKKWIEDNRHLMKPPVGNKCIYDGDFIVMVVGGPNSRK
                     DYHFQDGPEFFYQMEGDIQVGIVEDGKETIYDIKEGEIFYVPPKTPHSPRRPADTVGL
                     VVERKRTEEELDGFQWYCENCHQKLHEKFFKLTDIVSQLPPLLKEFYDSEELRTCKHC
                     GSVMQPPVKSN"
     gene            7490..7855
                     /locus_tag="PLX80_00050"
     CDS             7490..7855
                     /locus_tag="PLX80_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQY19214.1"
                     /translation="MIRESVIHKKSYSFSIRIIRLYQFLIKEHKEYSLAKQILRCGTS
                     IGANIEEATGGYSRKEFLSKLSISYREARETLYWLRLLFDTDYLNKEMFDSLFKDCEE
                     ILRILSKIQLTTKASLNIK"
     gene            7893..8897
                     /locus_tag="PLX80_00055"
     CDS             7893..8897
                     /locus_tag="PLX80_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY19215.1"
                     /translation="MKIDLHTHILPETWPDLKEKYGYGGFINLEHHKPCCAKMMKDGK
                     FFREIQNNCWDAKVRIKDCDETEVSMQVLSTVPIMFSYWAKPLDTLDLSKYLNDHIAG
                     VVNDFPDRFIGLGTLPMQATDLAVKELERCIKDLGLAGVEIGSHINDWNLDDEKLFPV
                     YEAAEDLGAAIFVHPWDMMGKDRMPKYWLPWLVGMPAETSLAICSMIFGGVFEKFKKL
                     KVCFAHGGGSFPMTIGRIEHGFNVRPDLVAIDNKINPREYLGKFYLDSLVHDKAALKF
                     LIDLVGDEKIVLGSDYPFPLGEHHPGKMIESMNELDQATKDKLLWKNAVEFLGIKVKN
                     "
     gene            complement(9007..9774)
                     /locus_tag="PLX80_00060"
     CDS             complement(9007..9774)
                     /locus_tag="PLX80_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19216.1"
                     /translation="MKNIISSIFNSDNNSPEFQKLIERWDNFLSKLKDRYYEILQQSD
                     APLQNVIDNLQYDTVVIHNIKTGLHNQTVDQLSKKAEETWPKMESEMQKIGVSWDQIN
                     EQHRKSDDLRQWMEWEFGIFEVTTYGKAARKILENVKRHIDEKKLHRCTQCAAELPIK
                     IYSFMAVNLKCDSCGSVNTYQPDDRIRALEYYVLNHLAEEQAYPFKQNAKSDKNAMKE
                     YYKTYYEFLMENVPDKKEFYKKDMEERINNPFFLNAF"
     gene            complement(9774..10280)
                     /locus_tag="PLX80_00065"
     CDS             complement(9774..10280)
                     /locus_tag="PLX80_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19217.1"
                     /translation="MSELNSSEGFKSETDQAFQKAWHSEKGGGIEWNDFMQTYEVSPL
                     PDSDKQVLLNSEIKITRGAFPIELRKVYEKIIEKYSGSSDTKVQSELIAFNIDQKVNE
                     YFQKIKPFSGMSDIFSNASATLNKYSEGMQNAKHHTMRCKNCASPRLEEMQYDSCLFC
                     GSKLFEKA"
     gene            complement(10293..10814)
                     /locus_tag="PLX80_00070"
     CDS             complement(10293..10814)
                     /locus_tag="PLX80_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19218.1"
                     /translation="MSANLEPVEGISLEQWAGVNASLASGGVIEEVIKSIGADMPKWD
                     RVNNEWLSRMKNDTSFTIATKYSAAFNTNAAGNLGSGSQTDASSIPFEKYVESMVAQD
                     VLGKQGRDAQDVLKDFGMTVADYSNVSSYWSGKMMSDFSLAAKMSQLMEEYKKKYESM
                     QGSGTGANDDIEF"
     gene            11363..11953
                     /locus_tag="PLX80_00075"
     CDS             11363..11953
                     /locus_tag="PLX80_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19219.1"
                     /translation="MTLENFLKEYDKEGVVVLLEGKRQVKKGDERKLQRLGQLLKERS
                     NYMIFRSGNAEGSDRYFFAGASSVDNKRLQAITPYDGHFKNSNSAYETFSLDSIDLVK
                     EPEITYLSKRNKKTKGLIDKYVDGNRDKYSIKAAYIIRDTVKVTGYENMKPASFGIFY
                     DDLDNPKSGGTGHTMDVCEEKNIPVIDQRVWFKWLE"
     gene            12013..12162
                     /locus_tag="PLX80_00080"
     CDS             12013..12162
                     /locus_tag="PLX80_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19220.1"
                     /translation="MSASRFFLFSKVILILVIFISLQSCSSEDNTVNQPTTGESNRTD
                     SYVSF"
     gene            12449..13705
                     /locus_tag="PLX80_00085"
     CDS             12449..13705
                     /locus_tag="PLX80_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19221.1"
                     /translation="MIYGKFYAGAFLFFVSLIIFQSCSDDGVVAPLNDDVWGIVVNGN
                     GNPIPDLVVRIGDQSTVTDMNGRYNLYDIQTPYDVLIKDESSRSQMLVKSVTSREILI
                     PDFITSYNPDFKAYVNVKIPSSIIQSGKKGKIIFTDGSGLNSTTDITSDSTTLEVNLK
                     YNSQTKGKLIVLIYSVDAAGRVFTYNNYASKTGIDLTNGSTYSFSFDTNDLSFNPPER
                     IINGSINIPAGYNSSSQYYYLSFADRNISFNDPSCKFADITGDNFNIVAPYDLPDVFT
                     VMVNNYSNNSDKYSLETFVIHDYENSVNLSSHIPVDLISPANNISGITSDSEISFSQG
                     DGTGVYAVRFIKDGYIYSIITSATSFPFSELEKFDIGNIKNEHFIWTVQKYGHTSGLR
                     EYLNVIPIIEVKFLTKSEQWMFQAAP"
     gene            13898..14407
                     /locus_tag="PLX80_00090"
     CDS             13898..14407
                     /locus_tag="PLX80_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19222.1"
                     /translation="MHSAYLITQLENNADVFKSLLSGISVEMIKWKTSEMNWSLLEII
                     CHLYDEEREDFRARLAKILLEDHEWDPIDIQTRVVSGKYSEKDFTTVLNDFLKERKLS
                     IEWLKNLEVHDWNIKAVHPKMGEFSAIQMLANWLAHDYLHFRQIVKTKYLYLQNSISP
                     ADLGYAGEL"
     gene            complement(14419..15129)
                     /locus_tag="PLX80_00095"
     CDS             complement(14419..15129)
                     /locus_tag="PLX80_00095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY19223.1"
                     /translation="MKIKDSRILITGGSSGIGKAAAVLLSQSGAKVAITGRDKDKLDK
                     TAEETGSLAIHADVSNEDDIKKTYEIMMKEFGGLDCLVNNAGIGEFPEVEDLTMEAFQ
                     NVYSVNVFGAALMTKHAADIFKKQNYGNIINIASTAALKGFARGTIYSSSKFALRGMN
                     ECWQAELRPHNIRVMLVNPSEVTTAFANKERKERDEVANKLTSVEIAHTIKSLLEMDD
                     RGMIPEVTVWATNPWDKK"
     gene            complement(15140..16207)
                     /locus_tag="PLX80_00100"
     CDS             complement(15140..16207)
                     /locus_tag="PLX80_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQY19224.1"
                     /translation="MRKESLEFLKNYLNISSPVGFESPGQKLWLEYLTPYIDTHFSDV
                     YGTVVGVINPDAEYKVVVEAHADEISWFVNYINDDGYIYVRENGGSDHTIAPSKRVNI
                     HTDKGFVKAIFGWPAIHVRDKKGDQPEKKNIILDCGSKSKKEVEALGIHVGSVVTFED
                     EFMTLNKNYYVARAMDNRIGGFIIAEVARMLKEKKQKLKFGLYIVNAVQEEVGLMGAQ
                     MISRRIKPDVAIITDVTHDTQSPLYDKIKDGDIACGKGPTLTYGPSIHNELQKMLIDI
                     AKKKKISFQFEAVSRSSGTDTDAFAYSAEGVASALISVPTKYMHTTVETVHKDDVENS
                     IKFIYEFLINLKNGHKFSNFK"
     gene            complement(16220..16897)
                     /gene="rpiA"
                     /locus_tag="PLX80_00105"
     CDS             complement(16220..16897)
                     /gene="rpiA"
                     /locus_tag="PLX80_00105"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003738577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HQY19225.1"
                     /translation="MKDIQKEAEKKLAAEKAVEFVKDGMTVGLGTGSTVYYAINKIGD
                     LVKDGLKVKAVSSSNASTELAKSLGIEVIPIDDVPLININIDGADEADSSFNGIKGGG
                     GALLFEKILALNSEKNIWIVDSAKLVNTLGKFPLPVEVIPFGYKRVLEHLKDCNPELR
                     LKDGNIYKTDSGNYIFDLHLNVIEDPLIMDMELKQVTGIVETGLFIDIADTIIIGKEN
                     STEIINK"
     gene            complement(16955..17836)
                     /locus_tag="PLX80_00110"
     CDS             complement(16955..17836)
                     /locus_tag="PLX80_00110"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQY19226.1"
                     /translation="MKNLILLFAFLFIFTELSFSQSDTTKFKRTRNTDTTYYKKGWTP
                     LGTIGLGVNQVALTNWTKGGENLLTITGNSDFYAIYYSKPWLFKNNLSFTLGSTKTGS
                     GSFQTNDNAAYFENVLIRKIGWALDPYISNEIRSGILNGYDYSLDPIIQTSAFFDPGY
                     ITQSIGLTYDRPMFSSRFGLAFQETFANVYRQYTDDPNTPGETEGFKFQTGMESVTDA
                     NFVVANNLLFSTRLRLFSAFDQLDVWDVGWDNTLTAVINDFMTASLDVLLVYEKSQSL
                     KTQLKEGLQLGVNYTVF"
     gene            complement(18097..20571)
                     /locus_tag="PLX80_00115"
     CDS             complement(18097..20571)
                     /locus_tag="PLX80_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19227.1"
                     /translation="MKLFLILFSILTLATPVIYAQDEEGETSETILQREFYITDRRAG
                     GPGQVLPFDAFAKAVQQKKMMTRDKMDINSPTSLTGWISVNPTGMFYSRTNNNFIAGR
                     TNSIAFHPANPNIFYIAAAQGGVWKTTDGGVNWTVLTDTLGSIASGDIILDPSNPDIL
                     YYGTGELNYSSDSQYGDGVYKSTNAGMSWFKIATSAQIGTRTGKMVIDYVNTNNIYLA
                     GINGIFKSTNAGLTWTNQLFLNSTSLIIDPVNPQILYASSGNVQSQIFKTSNGGTNWT
                     LLTNGLNTSNGRRTQLAISPDNPNYIYASIASTTGSLLGLYRTTNGGDLWTNQNSTTN
                     YMSSQGWYDNAVTVVPGDPNKVIVGGLDIYRSTNGGVTLTKMTVWSTSSQSDFAHADI
                     HYLSYKGSVLYCCSDGGVYISPDDAETWTDLNMNMSTLQFQSADYDPTNPQFMYGGTQ
                     DNNKQTSTDGGLFWNQRTTGDGGYTIVDPVNTNFVYGQYVNGSIQRSANFGAGFTNIT
                     PSGSTGGLFYNPYEMAPGDHNTVVFGRADVWKTSGVQTASTSTGWTQIATTGDIGGSV
                     SAIGISNTNTDKIYIGTSNGRIHVTTNNGANWSSQTGFPYISDFWVDRSNDEICYAAF
                     GGANADKHVYKTTNGGTNWFSISTNLPNIGTNSIVIKETNPKMIFVGTDLGVFRSTND
                     GVSWTSFNTDFPNVEVYDLKYKEGPKILMAATHGRGCFTFDIDGILGISNINSIPGNF
                     NLNQNYPNPFNPSTSISFTIPVRNFVTLKIYNTLGKEVYSLINKEYPAGTYEYKFDAS
                     DLSGGVYFYKLTAGDFTASKSMMLIK"
     gene            complement(21264..24602)
                     /locus_tag="PLX80_00120"
     CDS             complement(21264..24602)
                     /locus_tag="PLX80_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY19228.1"
                     /translation="MNDLGYKRFPTVSGDKVAFVSEDDLWEVNLSGGIARRITSNSGE
                     VINPYYSPDGKYIAFTGKEEGENDVYLIPSQGGMNKRLTYLGGFLKVKGWTPDSKYVI
                     FSSDTKQAFTGMNMLYKVSVDGGMPELLPYGKGQNIAFGPKGGVLIGRNTLDPARWKR
                     YKGGTAGVFWIDKKGNGKFEKFLTKVKGNLASPLWVGDKIYFISDHEGIGRLYSCDID
                     GKGIKALSKNTEYYVRNATTDGNNIVYHSGADIFVYNIESDTENKIDIEYYSPQIQRQ
                     RKFADAGKYLESYNLSPDGKSTTITSRGKSFMFSNWEGPVLQAGKKLGVRYRLTQWMY
                     DNKSVVTVSDETDFGTIEIYNSLEGNSEVKKLDFFKAGIIANLKVSPKEMKIAVSNNR
                     HELFIIDAENMSFKKIARSKFERIEDFSFSPDGKWIVYSMSNLEHLSSLYLYNLETGK
                     SSQITPAGFRDYQPVFDPDGRYIYFLSQREYNPTYDTSFFQLGFTLGVRPYLITLRKD
                     IQSPFTPEYDKLFARERNNGKEKNREKDSDNNKDKKVAKENKKKSKEVLIDLDEIDQR
                     IISFPLPEKNYFQIAAVKDGLLYSREPIKGALHHWYWDEHPSDESLDLFDFHTQKNEH
                     VAKKIKSFRVSMDGSTLIYRQEKKLYINSKVLAEKHHDHHGDHDDDYSGHGRGKNDWL
                     DLSRVKINIDPISEWKQMYSESWRLQKEHFWTPDMSGINWDNVYKRYLPLLDKVASRS
                     EFSDLIWEMQGELGTSHAYEMGGDYKPSPTYRLGKLGADYEFDEKENAYKITHIVNGD
                     SWKKNSPLNAPGVEVSVGDYLIAINGDRLSKNILPEELLVNQAKNIVSLLVKNSKTGE
                     TRSFNVKTIASEYEPRYREWVEKNKDYVHKKSKGKLGYLHIPDMGPNGFSEFHRYYIH
                     EAGMNGLVVDLRNNRGGHVSQLLLEKLSRKQYGYDLSRYGSVGTYPSHSVAGPMVALT
                     DEHAGSDGDIFSHQFKQMKLGTLVGKRTWGGVVGINPRLMLADGTMTTQPEYATYMKD
                     VGWKVENYGTDPDIEVEITPEDYVKGRDPQLDKAIEIALDQLKKKPVIIPDFKDKPLL
                     TPMIHLNGNGKAKSNSKTKNKIKQ"
     gene            complement(24880..25740)
                     /locus_tag="PLX80_00125"
     CDS             complement(24880..25740)
                     /locus_tag="PLX80_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY19229.1"
                     /translation="MENPSVIILGIAQDAGYPHAGCKKDCCKDAWKDKSLQRSAACIG
                     IADPQSESYWLIDATPDIKFQMNMAEEKFKECKLKGIFLTHAHTGHYTGLIHFGKEIM
                     GIRMLPVYVLPEMKNFLMNNHPWKLLENSGNIELRELKFDDEIVLNERIAVQPFEVLH
                     RDEYSETAGYQINFNSKKIIYIPDIDSWEIWDKDILKIIKKSDALFLDGTFFTKEEIK
                     NYESKNIPHPLVSDSIKKFEELSLKEKSKLHFIHLNHTNPLIRGDSEESKYIISKDFK
                     IAFEGQIIEG"
     gene            complement(25948..26166)
                     /locus_tag="PLX80_00130"
     CDS             complement(25948..26166)
                     /locus_tag="PLX80_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQY19230.1"
                     /translation="MKKEYLIIIEKGKNSYSAYIPDLPGCIATSDSEKNLLKLMEEGI
                     ELHIKDMKKRGYKIPLPTTKSAVIETAA"
     gene            complement(26163..26360)
                     /locus_tag="PLX80_00135"
                     /pseudo
     CDS             complement(26163..26360)
                     /locus_tag="PLX80_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227169.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(26537..26686)
                     /locus_tag="PLX80_00140"
     CDS             complement(26537..26686)
                     /locus_tag="PLX80_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19231.1"
                     /translation="MPAVKKTAVKMHGKINLFKGQQHSLSSGVYFYELVSGDFKAVKK
                     LIVLK"
     gene            complement(26927..27376)
                     /locus_tag="PLX80_00145"
     CDS             complement(26927..27376)
                     /locus_tag="PLX80_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19232.1"
                     /translation="MNLSFKLIFLVFLFIVFNALNISPVNSQTQQIRKVEYEGELVEY
                     FKNKLILYLQRDKAAESTYLKEVNEYINLHGGKLDSISKVSIWYIAEIQFADSSADAV
                     NIIDDFKNSGLFEYVYLDFVIRLNELFDPNNSVYSENLYLKIHYKKF"
     gene            complement(27751..29940)
                     /gene="katE"
                     /locus_tag="PLX80_00150"
     CDS             complement(27751..29940)
                     /gene="katE"
                     /locus_tag="PLX80_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HQY19233.1"
                     /translation="MAKKKPAKNENGNKKTDDLGQNTENGSGEFLTTNQGLRINDNQN
                     SLKSGERGSTLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQVYESMSKYTRAG
                     FLQDPGKKTPVFVRFSTVAGSRGSTDLARDVRGFAVKFYTNEGNFDLVGNNMPVFFIQ
                     DAIKFPDLIHAVKPEPHNEMPQAASAHDTFWDFISLMPESMHMIMWVMSDRAIPRSLR
                     MMEGFGIHTFRFINEKGKSVFVKFHWKPLLGVHSVAWDEAQNISGKDSDFHRRDLWEA
                     IENGAFPEWELGVQIVPEEDEHKFEFDLLDPTKIIPEEIIPVRRIGKMTLNRNPDNFF
                     AETEQVAFHPGHLVPGIDFTNDPLLQGRLFSYTDTQLSRLGSPNFHEIPINRPVSPLH
                     NNQRDGHMRQMINKGQVSYEPNSLNGGCPFQAKMSEGGFTSYAEKIDAKKIRGRSKSF
                     FDHFSQAALFYNSQVQYEKNHIINALRFELGKVETVEIRERMVGLLTQVDKTLAASVA
                     EGLGMSLPSGPMQPVNHSIPADGDPKKFQPHFVKQSVQKSEALSMENTIKNSIKTRQI
                     AFLAADGVDESSINKMKKALETEGAQIKIIAPSLGKLNNKNGKGINVDQSFLTAASVL
                     FDAVYIPAGKNSADTLSKNADAVHFINEAYKHCKAIAADNHGIELIKSSNIIGKMNDE
                     NSELKNPAEMGVIINSNGYSKNFIKDFIDSIAQHRFWQREDKNNVPA"
     gene            30300..31448
                     /locus_tag="PLX80_00155"
     CDS             30300..31448
                     /locus_tag="PLX80_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQY19234.1"
                     /translation="MRLKSKLPDTGTTIFSVMSALANKHNAVNLSQGFPDFPVDPILS
                     ELVFKASRDGHNQYAPMPGLKSLRDGISNIISEIYGVHKDPDKEITVTSGATEALFAA
                     ISTVVNPGDEVIMFDPSYDSYEPAVILNGGISVRIPLTFPSYTLDKEKIISAVNKNTK
                     LIILNSPNNPTGALISKEDFNFIKEIVLKNDLYLLSDEVYEHIIFDGLKHYSVLPDEE
                     LFKRSFVVSSFGKTFHITGWKVGYCTAPEELTDEFRKIHQFLTFSTSTPFQFALTEYI
                     KDIGKITALKNFYQSKRDYFRGLLERNGLKPFLCSGTYFQLIDYSSISDEYDLDFAAR
                     LTKDFGIASIPVSVFYKDKTDNKVLRFCFAKEDKTLDEAAECLKRILR"
     gene            complement(31463..32365)
                     /locus_tag="PLX80_00160"
     CDS             complement(31463..32365)
                     /locus_tag="PLX80_00160"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY19235.1"
                     /translation="MIISVLEIYFLEKKFRRLKFSTSVILRSLLYIFIFIGILLLILK
                     INMGLEHNDTFQEKVKKFGTFTNFIESKSFFVMLAVGVLMIFTINFIRQINRLMGQNV
                     LLNYVTGKYQLPLEEELIFMFLDLKSSTTIAERIGLEKNHELLNDFFYDMTDSILECK
                     GRIYQYVGDEIVITWRMKDGINNLNCIKCFFKIKNRIAGKKDQYMKKFDVFPEFKAGL
                     HFGKVITGEIGDIKKDIVYHGDTVNTSSRIQYECNGFGKSFLISETLLKKMNLKEEYK
                     TESMGKIKLRGKSEELELYSIEEN"
     gene            complement(32604..32918)
                     /locus_tag="PLX80_00165"
     CDS             complement(32604..32918)
                     /locus_tag="PLX80_00165"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19236.1"
                     /translation="MNFKFSPHIAIQVKDYEKAKGFYENVLGMKIEKTSEKETHFVKD
                     GINFYMENSEGGNTFFEFKVDSIDEALKILEKEGCKVTQVYSEKSKMIADPYGMRFHV
                     WE"
     gene            complement(33284..35815)
                     /locus_tag="PLX80_00170"
     CDS             complement(33284..35815)
                     /locus_tag="PLX80_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQY19237.1"
                     /translation="MKYIVLLFLLLLIPEYIFSQTKPDTSQSGFSGKIRVVAGEKYDI
                     SGFSEIFLGKHWRDLWTTPFDVNILNLNTFAGGLTPYKRGGGLQTKSLRLKGEDGKVY
                     KFRSIDKDPKMILPKDLQETVFADAVQDQISTSNPLASIIVASMLNQVGIINAVPIIV
                     YMPDDPRLGEYRKDFANVLGTFEENPESGDEDEKGFAESDKISNSYKLYKKLENDNDN
                     QVDDIEFLKARLFDLMIGDWDRHSDQWLWAGFEEDGKMIYKPIPRDRDQAFCLYDGIV
                     PFFVGEYVTQIEGYGENYPRIYDLSWNGRYIDRKFLTQPTKKQFDSLARYIQSKITDE
                     VIVNAVKQMPAEWYSLEGKNLISMIKSRRDQLPEATDEYFELLNSVADIYGSDKREFI
                     EINNISDKKIEVKMFKQDKETKEKKGEPFFKKIFDSEYTDEIRIYLNEGKDILEFKGE
                     NNSGIEIRVIKDNENLTVKGNKDNGIRYYKDDRPKDNIQYRNEPPVEDRGNEWRFTPV
                     FNYDSDNGIELGGGPVYYKYGFRAKPYVYKMSLTGSYAFNSGSYNFKYSGDFYSIIND
                     KRINLEVIQTQLAITRYFGEGNETPFTDSLQDQKYYNVKQGLFKVATTFYFPFRKDLE
                     LTLTPFYKMPNVSYDQNTLLGQNPNTYGIGRLNFAGINTSFNYDTRDNTMEPYKGIYA
                     QALGNWTPKIFDNNFGFGKAGVDIRAYISTDTVKGVTFAFRTGAGKVWGDYPFYESMF
                     LGGANSLKGFSRERFAGDAVVVAQSEIRMSVARVNILVPGMLGLSVFGGTGRVFLTGE
                     ESKRWHSTFGTSVWITYLDRMFNIGLTVAKSDEGYKFFVGSGLFL"
     gene            complement(35962..37386)
                     /locus_tag="PLX80_00175"
     CDS             complement(35962..37386)
                     /locus_tag="PLX80_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY19238.1"
                     /translation="MRILITGATGYIGQRLLPALIQNGHEVICCVRDVNRFDADKYGT
                     GKLKVIEVNFLNKESVSLIPADIDAAYYLIHSMSALNEDFESLEKVTAENFRNRIEKT
                     NVKQVIYLSGIINDKKLSKHLRSRRAVEKILSDSDFHLTTLRAGIILGSGSASFEIIR
                     DLVEKLPVMIAPKWLSTKSQPIASRNVVEFLTGVLLNEETYDKSYDIGGPEIMSYKDM
                     LLRFAKVRNLIRKIITVPVMTPKLSSYWLYFVTSTSFTLATNLVKSMGVEIICKENNL
                     RELLGIKLLTYEESILLAFNKIQNAEVVSSWKDALSSRILGEGISKHINVPTFGCYID
                     KRETVVKNVDATLNKIWCIGGRNGWYYGNWLWEIRGFIDKLFGGVGLRRGRKNTNLIN
                     AGDSLDFWRVLYADKREMRLLLYAEMKLPGEAWLEFKIENNVFYQTATFRPLGLLGRI
                     YWYAVMPFHGFIFGGMMKKIASAE"
     gene            complement(37444..37776)
                     /locus_tag="PLX80_00180"
     CDS             complement(37444..37776)
                     /locus_tag="PLX80_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY19239.1"
                     /translation="MTKSEIIRKYLGSYETGDKNTIENILSEEFIFSSPQDDHIDRAA
                     YFEKCWPMNTQKPFIPIETIVESGEEAFVIYDCNMSGMKFRNTEYIKFSADKIISVEV
                     FFGNEIKN"
     gene            38197..38655
                     /locus_tag="PLX80_00185"
     CDS             38197..38655
                     /locus_tag="PLX80_00185"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HQY19240.1"
                     /translation="MKKIILVVFAIAIFSNINAQDIKETDVPEAVKTAFYASYPDADN
                     VEWEIQGAYFEGGIKVNDMVTSVLLDANGFLVQTESQIPASDLPDGVKDYISKNLNNA
                     QIEEAAKITEPTGKVTYMAEVKNSEYMFDPEGKLLYNLQGDSDSGEKDSD"
     gene            complement(38663..41923)
                     /locus_tag="PLX80_00190"
     CDS             complement(38663..41923)
                     /locus_tag="PLX80_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQY19241.1"
                     /translation="MFIPIIKFELRLWLKSLSFYFYSFVFFLAALISLSGAAGIFGEG
                     SGSTGIANSPIDILSTAIFFNKLFLLLIPVIIGTVISRDYLSKTNRFLFSYPVKKSDY
                     LSGKFTNGFITVIFISILVMSGMALGLLLPGINSDQVIGFDLSVYLQTFFLYLLPNLF
                     LVTVIVFTVVLRTKNIYSGFPAVIILLLLTKLISGIMFSTGFGFPEITADPFGEIYFE
                     NKIKYLSASELASISIPFGFEYLINRIFWMTIASLVLCYTYKKFSFTVTEKVKERSFF
                     KKKELLSESKTSYRSLDIKFNYTFSQNIRSIWRLSQYDFNYITRNLSFRILALIGAVF
                     IVVLILKGNTDTGVKLLPVTWTIMSIPFLFYSLLIIFLTFLYSGLLINRSESTGMKEL
                     ECMTPAPDFVLYFSKLTALIKIQILMLLIMMISGILIQIYSGYYNFDIGNYIYTLFGI
                     HLIGFVIWAILALFIQTLVTNSFTGLIILVLIFLGLDNVPIGDAGSLVLKFNRNPEPD
                     FFLKYSEISGFGHSVYPYFVSKLYWVLFCMVLINLTIIFNKRLKADLISERIKNVNLR
                     FYGKKLMPLTGLMALFILSGIYIYSEKKNESEIILTEKQKSEYMLNFQNEFGKYDSFP
                     QPRIVLLNFNMDIFPDEKSFTAEGFYILVNKTNYVIDKLLLKSGYDENTSYTFSEGTE
                     LEAYDSVLKFSVYKLSKGIEPKDSIKLYFSIKNISNTILTQNSNVLEDGTFIKSNIFP
                     HIGFINLKNENKLNPEDSISHLNHYQRYDSDLINIEATVSTSKDQIVIMPGILMDKRE
                     QNGRNYFHYKSEGNIKFVLGINSGEFNIYQESYKGKELSIFYYKDHSNNINNFADGIK
                     SAFDYNTEYFGEYQHSNISIVEFPRSEGSYATTSGNTIQISEIRFINDTGEDGEKNTD
                     ISFYTAAHELTHQWWGNQIIPADAAGALMITESITEYITAKIYERKFGREKAMGFLKI
                     QYDRYLKGRAEEVDKELPLMYVSPEQTYISYGKGSIAFYILSEFIGEDNLNRILRSFL
                     EKNKFQGPPYTTTHEFIRYLKEETPENLHYLIYDLFESSDLEKFSEYYKKLDGNSN"
     gene            complement(41923..42810)
                     /locus_tag="PLX80_00195"
     CDS             complement(41923..42810)
                     /locus_tag="PLX80_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19242.1"
                     /translation="MKFSINNLSKSYERSHKALDNINIEIGQGMFGLLGPNGAGKSTL
                     MRIAATVQLPDEGEIYFDEINVLEDPMSYRKLLGYLPQEFGIYQGMNADELLDYFAIL
                     KGISSSRDRKMITDKVLEMTNLLDVRKMHVSKYSGGMKQRFGIAQILLNDPKIIIVDE
                     PTSGLDPAERKRFLNLLREIASTNIIIFSTHIVDDIKDLCNDMAIINKGRILKTGSPT
                     AAVKELDQKIWVINIDKNELEEYTRLHRVISSKFNEDNSLSVKVYSEEKPSAEFIDAD
                     PDLEDIYFTVLENDKHTVS"
     gene            complement(42996..43472)
                     /locus_tag="PLX80_00200"
     CDS             complement(42996..43472)
                     /locus_tag="PLX80_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19243.1"
                     /translation="MCKKLTDLFLPVIIIFVSIISFIGCSSEDNSVDPPATGEVLLAE
                     VSGDSVGVSSGSSSMSQSITGSTLNFSDRDSVRLTFYYSGENNFDPAPLKIFYNQGPA
                     EINIFNFSSANITPTEKYIDTTIASPKVNDFFIYRITASSPTFSFFKFRDLKIYKK"
     gene            complement(43574..44200)
                     /locus_tag="PLX80_00205"
     CDS             complement(43574..44200)
                     /locus_tag="PLX80_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19244.1"
                     /translation="MEPKDAYRSWSEQYDTNINKTRDLEGITIRKELAGMSFKNCLEI
                     GCGTGKNSEWLLTISDKLTAVDISPEMIAKAKEKILSDKVSFIQADINNEWIFVKEQF
                     DLISFSLVLEHIEDLNHIFFEVSKALSNEGKVYIGELHPFKQYTGTKARFETEKGIQT
                     VTCFDHNISDFTNSAKKNGFILSEVNEYFDKEDRTGIPRILTLLFRKK"
     gene            complement(44263..46056)
                     /locus_tag="PLX80_00210"
     CDS             complement(44263..46056)
                     /locus_tag="PLX80_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQY19245.1"
                     /translation="MKLTEEQKEIISSEGNIKINAVAGSGKTTTIIEYAKARPENSKI
                     LYLAFNRSVKTEALKKFAAKKLYNVKVETAHSLAYGHVVYGHGYRVSAKGYKSHEICE
                     ILHLSGVGEKHGEHIVANHVSKFISYFCNSDKQKVSELNYADVVSDNRANAFVSKHYD
                     YIENQTRLFLSKMNSGEIEITHDFYLKKFQLLNPKLNYDYILFDEGQDASAAMLDVFL
                     KQDAVKVIVGDTHQQIYGWRFAVNSLEKAEFKTFSLTNSFRFSQDIADLAMEILKMKS
                     QVAEFKPSPISGKNLSTQSGTSAVIGRTNLGLLVKAIEIVTGNKKIRSLYFEGNFNSY
                     TYSEEGASLYDVLNLYNGKHKLIKDKLIKQMRSLSELQNYINKTGDSQLSMLVEIVKE
                     YGNDIPQILKKIKELHVSDDKKEKAEVIFSTVHRCKGMEYDTVQIVNDFINEEKLEKQ
                     IEEIKKTGSGLSKINEEINLLYVAVTRTKNCIEIPDTLLPKGFPDSQRIKKIIGEPVS
                     GNKDNKNYSKISYEKPDFSGSKKSKVYSADKVREKFKSAYTPWTEKLDDELTVMYCEG
                     ANVKKIAKHFGRTLNAIRMRIEKLELEDIYG"
     gene            46452..46907
                     /locus_tag="PLX80_00215"
     CDS             46452..46907
                     /locus_tag="PLX80_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19246.1"
                     /translation="MKYFITIIILTATFYYSCSNDSVQDPNAITEDYTSILNTDKFGN
                     ILGGDTTDWCKDTCSNALQPCDAFSPAYPNPVNDTLFFRFSVSSNLSNLKLYFLNSSS
                     DTLFLANGVYDAGHYVLFTTASILGYSNVYKRAYLKINDFSCYGDIKFN"
     gene            complement(47138..47557)
                     /locus_tag="PLX80_00220"
     CDS             complement(47138..47557)
                     /locus_tag="PLX80_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19247.1"
                     /translation="MEKKIGVWLDHSKAKLIEIMESGSEITTVHSPYKRHVRIPGEDS
                     SSVETRFGKKYFSNTEFKLHQKKQNEVASYFNDLEKRLLKYDSILLFGPTLAKSELNN
                     LLSKNKKFSKKSITLKNADKMTENEMAALVREHYEKK"
     gene            47765..50113
                     /locus_tag="PLX80_00225"
     CDS             47765..50113
                     /locus_tag="PLX80_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY19248.1"
                     /translation="MTGTNKSTSVQKYVFITAAVVLGILSLIAPIWPDGHIEPRVGGL
                     LIVAGILEIFHGFRRSTDEKRKAAWFSSAVTITFGILLINASLLISTALLIFIVITFC
                     IDGIRYGAEAFKNFKNKKDYLYDVLAMIGNFSVIAVILFTKNFGADWTIAIVGAWRIF
                     GTALNIYHAKEGNYRSSGEDVIKSLHLPDTRSVKSAVSKIQSEEDARYPADKSWIIVF
                     LLLLFLIHLGRMGFDKTSLGIFSPAVALFGDIVVALIITFAIITPIRALLKKLTIPVT
                     RKLWLWVDKVPSDQRKKFGPRKIVNAYLEHRLRVSIRLRNAGYSFKSAFMTGMKTGLP
                     YAAMLAAVIPVFGMSWYFDTENWAAGVWDSWAAARTDNWRMAITRSVGEPPGPEAFLL
                     HPEGVNGNSDFSFIVIGDPGEGDASQLSLKDQIQIVSEKPDVKFLVISTDIVYPSGEM
                     KDYENKFWLPMKGIYKPVYAIPGNHDWFDALEGFSATFFEPQAAFDGMKARVTADLKI
                     SASTDDHINGLIKEADRLRNNYGVPTGFQRSPYFQIQTDKFALITIETGILRRIDEDQ
                     MTWLKQALEASKGKYIMVLIGHPFYAVGEYMGDLNPDFKDIHQLLRQYKVNLVMGGDT
                     HDLEYYVERGYDDDPDSKMIHFVNGGGGAYLSIGAALKPKDEMPEKEWAIYPATVPLL
                     MKIEENNNILKEPAWYWTKNLNGWPFTPEFLSAAFDYNNSPFFQSFFQIKVEPSNGVI
                     RFIPYGVNGQLKWKDIETSGNVRPTDKTPEDGAEWVYALVGN"
     gene            50583..51104
                     /locus_tag="PLX80_00230"
     CDS             50583..51104
                     /locus_tag="PLX80_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19249.1"
                     /translation="MKPDLNRYIQLLEKAPVVLSDLLKDLPNELTRSNEGENTWSPFE
                     IVRHLIEGEKTDWMIRIKIILSDNEDRSFESFDRFAQITDSKDKSIEELLDIFRHLRK
                     DNLRVLSELDPGEKDWEREGIHPDFNKVTLSELLSAWVVHDLNHIAQITRVIAGQYKN
                     SVGPWIEFLPILK"
     gene            51282..51548
                     /locus_tag="PLX80_00235"
     CDS             51282..51548
                     /locus_tag="PLX80_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19250.1"
                     /translation="MPTIVVRHKVGDFDTWIKGHEDRVNVFAPAVSSFKTFKDADDPN
                     SVILVMEVNDMEKLQSILNDPSTKDLKDKHTVLEPITLSMQVDA"
     gene            complement(51679..53130)
                     /gene="sthA"
                     /locus_tag="PLX80_00240"
     CDS             complement(51679..53130)
                     /gene="sthA"
                     /locus_tag="PLX80_00240"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HQY19251.1"
                     /translation="MPKIKTYDLIVIGSGPAGEKAAVKAAYFGKKVALIEKSPETGGA
                     GVQTGTLPSKTLKETALYFSRVYEKHIFESTEKHSAHTKYKQFTYRRDIVRNDMQNEV
                     DKNFSRHKVDVYTGFASFIDAHNIQIQCENENVIEINGKFILIATGSYPVNPPEIPFD
                     RKRILDSDTILKIKRFPKSLCVIGAGVIGCEYATIYAAMGVKVFLVNNRDKILPFLDS
                     DIADALVRQMKIHGIEILFNTCITKYSKPKKASDSIHMTLKNGDELESDMVLYAAGRS
                     GNTKGLNCDKAGIIMGEREAIIVNEYYVTNIPHIYAVGDVIGFPALASTSMDQGRVAI
                     SHMFGTNDLDFVQKIFPYGIYTVPEVSMVGMTEQEALSNGIKYNTGVSRVGDIARGKI
                     QGSPDNGFLKLVFESESKVILGVHIIGRIATELIHYGMTLVHDKKTLDQMIATVFNYP
                     TFHDLYKYAAYDGLGNLSGKNIKNPLKIKNFFK"
     gene            53359..54051
                     /locus_tag="PLX80_00245"
     CDS             53359..54051
                     /locus_tag="PLX80_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19252.1"
                     /translation="MLELKNVNKDFYEGSAKHSVLNNFELSVPEGEIIILLGKSGSGK
                     STLLNIISGIDVPDSGSVKIDGTDITKLSEKDRTLTRRNKIGFIFQFFNLIPTLTVKE
                     NLQLPLELNNDDDQEMRINSILTEVGLLNRANSYPDKLSGGEQQRIAIARALIHNPDI
                     ILADEPTGNLDYETGLQIVDLLDRVVKKKGKTMIMVTHSKDVIGLADRIYSLKEGKVI
                     ETKEGKVGLDIE"
     gene            54044..56581
                     /locus_tag="PLX80_00250"
     CDS             54044..56581
                     /locus_tag="PLX80_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQY19253.1"
                     /translation="MSNLLTRSSLRFLLRHPWQFGLSVLGIALGVAIVVSIDIANYSS
                     ATAFNLSMNAVAGKSTHQITGTAEGIPDSLYRFFKKEKGIRNIAPVIESYVSIPDSNK
                     RVFKLLGVDIFAERPFRDYLSQTGISLNGGLKDFLTLPNSVILSKESLDKLKKVIGDT
                     LTVLINGKQKVLYITGIINEDEQNRSALENLFITDIASAQELTGKNDKIDYIDLIINN
                     VQEEQQIKSLLPDGYQLQKSGSRSETAEQMLEAFNINLTSLSLLALIVGLFLIYNTMT
                     YSVVQRRVLIGTLRSIGVTSKEISGMILREALIMGIIGTVTGFIAAYFISKILIIFIS
                     QTINDLYYVVSVREIYISPMIILKGAGLGIIATLLSAIYPAREASRVHPRSAMIRSEQ
                     ESSLLRNVPRMSITGVIFMIAGAVILLIPSHNIWLSYFGILPVIIGFALMAPLIIIMV
                     EKIFSPLYKKLFGITGKMASRSVLQNISRTYIAIAALALAVAATVGVGIMIGSFRSTV
                     INWLEARLNADIFISAPSLISRRNDAVLPEEILSKIKALDEVSDYNFYRELILPQDGK
                     EYHIIASGISKRSYSGFQFKEGDPNEVWEKFENGEVFLSEPFAYKNDIGLGENIKLKT
                     DNGLQDFKVAAIYYDYASDQGLITMNYDQFRKYWNAEGISGISVFVKEGNTIDSVKDK
                     IQTLGTDGQQLVVRTYKYLLDSSVEIFDRTFLVAKVLQILSVIVAFIGILSSLMSLQL
                     ERRRELGILRANGLLPSQLFKIVMLQTFLMGFTAGVIALPLGNVLAAILVYIINKRSF
                     GWTMQFEVMPSILIEALVLSIAAALLAGIYPGYKMSKTSPSNALKEE"
     gene            56584..57732
                     /locus_tag="PLX80_00255"
     CDS             56584..57732
                     /locus_tag="PLX80_00255"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQY19254.1"
                     /translation="MRKYLFVILIVFAVSGCKDEKTESGNGGISLSKAMSDTETGDMG
                     FEKAVEKRKFIFPDDHGPHPEFRTEWWYFTGNLTSSDNKQFGYQFTIFRTALNTDTNK
                     GISEWSSNQIYMAHFAVTDISGGKFFFDERFSRDGNELAGAQASPFKVWLEDWKINEI
                     NGAEGRTSFELPVLNIISKSDKSEINFILEAQKPFILQGDSGLSQKGNGIGNASYYYS
                     YTRLRTEGKIIIEGKKFDVSGNSWMDREWSTSALSGDQKGWNWFALQLDDGSEIMYYQ
                     MRKNDGTSDEFSKGTVIEKDGSSQLIKNEDVINEVTDYWESPSGEKYPSGWILKIPSQ
                     KIELKIAPALKDQLMNVSVKYWEGSVNIEGRKDDKEINGRGYVELTGY"
     gene            57932..58849
                     /locus_tag="PLX80_00260"
     CDS             57932..58849
                     /locus_tag="PLX80_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HQY19255.1"
                     /translation="MKLPYLPYDKWTDTRITLHLIFQIIGKIRLKTTPRKNHWWYVTL
                     YLSPKGFSTDQIPMSDGLNTFEIIFNVHKKAVQLYNSKGEEKEIPLENGYTVSEFYKN
                     IMGILNEWDLHPEFIDKPMDMITDKPFDKITEFHHYDWDSINLFWQMMLWNDGVFKEF
                     SGRFYGKTCPVHMYWHHMDLAVTRFSGKPAPPMPHDARISDKDAYSHEVISFGFWAGD
                     DNLPEPAYYSYTAPAPKDLDKESIKPEQAFWMDANGSPMAILKYKDLLNFDDPRKVLM
                     EFLESAYLSGAKLAGWDIEAFKVTPLKDL"
     gene            58871..59215
                     /locus_tag="PLX80_00265"
     CDS             58871..59215
                     /locus_tag="PLX80_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19256.1"
                     /translation="MQTFKILTSYKSIYKDPIVVIPGEIVKILEIEKEEKWKGWIKVE
                     STSNTGWIPIQIIEFSDDGNTGKILENYSAKELDVGQGDLIQKIYSLNGWSWSKKIID
                     NEEGWIPDEIIG"
     gene            59266..60084
                     /locus_tag="PLX80_00270"
     CDS             59266..60084
                     /locus_tag="PLX80_00270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19257.1"
                     /translation="MKKVLDWHTQEVVNIFDEASIWAAMFGRLLLENIPMKSEAKVLD
                     VGFGLGFPLIELSQRFGENSKIYGIDIWHEAIIRAKEKIRVLELSNIEIFEQSAEIIP
                     LPDNELDLICSNLGVNNFENRETVLKECYRVLKTGGSICISTNPVGTFKELFDLYHEL
                     LKEKNMDDCLLDFRKIIDHRGTEESIISEFELSGFKYSKKSTDKTFIKFTNAKALFDH
                     SLIRIGFLESWKTIIPSDKRNEFFDLLIKKIDGNILKNGNFIISIPMLYLEFKK"
     gene            complement(60127..61335)
                     /locus_tag="PLX80_00275"
     CDS             complement(60127..61335)
                     /locus_tag="PLX80_00275"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY19258.1"
                     /translation="MKNTLLTLIFIFSITNANADWIQQTSNTNETLLGVSFPDPMNGF
                     IVGTSGLILQTINGGNNWTVLPSGTTGFLRDIFFSDQMNGTIVGESGLILRTTNGGQN
                     WISQISNTTEHLVHVNFIDVQNGIIGGQNGVIITTTNGGATWTLNNSGVNSEIAGVNM
                     ISSTTAYAAGFNGTILRTNNGGLNWTSQSSGLTSELRSIFFTDQNTGFVAGYNGKILK
                     TTNGGDNWVSQNSNTANNLFSIYFSNSGSGTAVGTGKILKTTDGGDNWVSQTIPFTGD
                     LYSVFFVNENKGWTVGQNGRIFYTENGGVGINSNSDEVPEKFAIFQNYPNPFNPTTTI
                     EYAIPKASIISLKVYDILGSEVAVLINEYKQPGYYGVNFSADILASGIYFYKIEAGDF
                     IQIRKMILIK"
     gene            complement(61469..63232)
                     /locus_tag="PLX80_00280"
     CDS             complement(61469..63232)
                     /locus_tag="PLX80_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY19259.1"
                     /translation="MNLSKINFVIILILYGILFSGSTNSQTYTKITDPDNLVVNEMLE
                     SGGGFWVDLNNDGWLDLFVANGNLTSQNNSLFINNRNGGFFKVQSGPVVTDGGSSIGG
                     AFADYNNDGKSDLFVTNRNFFKNFLYTGNGDSSFTKIMTGSIVNDSANSNSGHWLDID
                     RDGDLDLFIVNFQGNDFLYRNNGQPAFDFTKIDTASFLLDGNAFSINAVWSDFNNDRL
                     PDLFIGNAGNQNDNLFRNNGNLTFNKTVIADGKSTLGGSWGDYNNDGNQDLFAANFLD
                     QNNILYRNSGPPLYELTALNTGIVSNDGGSSVGSCWGDIDNDGDLDIFVCNDDLQNNF
                     LYLNNGAPDYDFTKISAGSVVNDGGNSFGCVFGDYNNDGALDLFVANRLNQKNFLYVN
                     NSNSNNWIGIKCNGVISSKSAIGTKVRIKAQINGLPVWQLREIQSQSGYNSGNLILNF
                     GLGNAAVIDSMKIEWLSGTTSVFTNLQVNRYITVSEDGNITSVKDQSINPDFFELKQN
                     YPNPFNPTTIITYQCSSVSDVSLKVYDVLGNELTTLVNETQSAGSYSVTFDGSNFPSG
                     VFFYKLQAGESAETKRMVLLK"
     gene            63506..64171
                     /locus_tag="PLX80_00285"
     CDS             63506..64171
                     /locus_tag="PLX80_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19260.1"
                     /translation="MKFIYAKELNQMFDVTGHFYNLKLSGSVFKCRSVLEIRRKNSGE
                     ENVLPDIIVIMMNPGSSRPIDKNFKINTFSKKEYLSIKVRDVVPTRPDNAQYQIMRLM
                     IFNKWSFVRVLNLSDLRNGNSGKFKTEFINAMKLDKTNPHCLTHKDRSKELINFLKSK
                     NGKIIAAWGSISELKIPAENILKLNKNIIGIINGNVPNYRYASPYLKSQKTEWLKEIQ
                     KKL"
     gene            64347..64760
                     /locus_tag="PLX80_00290"
     CDS             64347..64760
                     /locus_tag="PLX80_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19261.1"
                     /translation="MKTLNAYLIFPGNCREAMNFYHECLGGEMVNMITFEGAPMEVEE
                     KYKNNIMHAQLKSDGIDLMASDGKPGGTHISGNNISLSLNVTSEAEQDEIFEKLSKGG
                     KIVMELEDTFWGARFGMITDKFGIQWMLNYHKPQK"
     gene            64786..65175
                     /locus_tag="PLX80_00295"
     CDS             64786..65175
                     /locus_tag="PLX80_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HQY19262.1"
                     /translation="MSYSFDKTLTDISFDDAITKVTDELKKEGFGILTEIDVKETLKK
                     KIDADINNYKILGACNPGFAHKAILAENKIGVFLPCNVIVMESENGDIEISAVDPSAS
                     MSAVNNESLAGIAVEVRDKLKKVIDQV"
     gene            complement(65205..>65783)
                     /locus_tag="PLX80_00300"
     CDS             complement(65205..>65783)
                     /locus_tag="PLX80_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19263.1"
                     /translation="ELLNRILKDLPDNIYTHFSKGLDEVFERSYDKLDYGRHFKMCLR
                     ENDFKEFNEANENKGLDYIKRLSPSDIDVISEFYKKSYPDNWFDLRMLETGKYFGYYF
                     EDELIGVAGIHVYSPEFKVAALGNITTLSEHRGRSVCRKLTSVLCRDLFKTTDQIGLN
                     VHSENLPAIKCYQNLGFTIAGEYFESTFKKKK"
BASE COUNT        20921 a        11810 c        10943 g        22109 t
ORIGIN      
        1 aaaaataaaa tatccttgtc tgagaaattt gcgaagcaag ttttgagttg gatattttat
       61 ttcagtttag aaaagaattt aggcaattta ttaccagccg gcgacttttt ttgttacttt
      121 cgagcctgtc ctcgagtgct cttatcgggg agtcgttcaa aaaaagtaag cagaaaataa
      181 tgaatcttga atattgtaat aaaacgtttc gaacaggacg gatatgaatt tatttaaacc
      241 cctttaactt atctccatta cattttcctc agtctctatt tttaacgaga catacataat
      301 gatctcatca gcattacaga tcttctcaac actcataact ttcaaaagat catcagtata
      361 ttcaggtttt gtcataccgg caagtacaca gtcaacacct tttactgaaa tcagaatcag
      421 cgcagctttt tgagaaagag taagtgaatg atattcgggt gagagttttt cattaatgag
      481 tttatttata aaacgactac gcttatctgc acgcagctta taataattcc ctgtaaactt
      541 caaaagcagg taaacattct gaatgtaatg attagaaaat tcctttactc cctcatcact
      601 gatatttttt tcaaaaaagt cgagaagtat attaatcctt ggcgcaaaca gttgtcttat
      661 caggtcatta aaatgttcta tgctcccgaa aaatttccag ttctcttcta tctgatttcc
      721 tgtaataaga ttgttcttta attttttata ctcttcctct ccaaattcat cttccgaaag
      781 tttttcgcta agcatatcct cttctaatag ctttacaagc ttgatctgtt ttataaaatc
      841 tttttcttca aatgcatctg ctttaaattc agcaagtctg acaagacctt tggaagtaat
      901 agcattcaga ggtctgttga tgagaacttt taattctttt ttcgctgcaa attccagaac
      961 tgtctttgtt ttattttcct gattcttatt tcttactgca cctgcttcaa ataaattaag
     1021 aggaagctgt atcattttaa atctgttatt ctctgaaatt gaatttgcaa tttcataaac
     1081 cttttctaat gaagtaaatg atctttcatc tgaataagaa ggaaaagtat ttgaagagat
     1141 cccataaaat tttatttctc ccgaatcaca tttttcctca agaaaacgaa atgctctttc
     1201 aattctttta taatattctt cacgcgaact ttccaggtca ggattatttg atgcagcgtg
     1261 atcaagataa tattcaggat tatgcagaag ataataatct atataaggag tattgagtct
     1321 ttcaaactgt ctcttcagct gatcttcaat aaagtcagga gaaatgcagt gccagagccg
     1381 gtctgactct tcgactactt cttcgaaata aataccattc tcttttttct gttttagttc
     1441 ggcaagaagt atcccctgaa tgtatccgcc tttagtaact atgcaaaggt cttctctttt
     1501 cagcttacct tcattgatca aatcattaac taccttcccg acgagaactt cacttcctcc
     1561 caccgaataa ttagaagatg tatcgatcag attaacgcct gaggtgagag ctttataaag
     1621 agaatcgtaa tgctcccgga ttttgtcact gatacggtag catccgaaac cgatctttga
     1681 aaacttttcc gtcataaaaa atttttagct tcaaattact tatattattt tgtgagtaaa
     1741 agtctaattc atactcactt tgtgaaatcg aaccgtttca gtggcttttc aaagaacaaa
     1801 ttttgtcatt cgtgatcttg caggaatcca ttacttgata gcacttcatc agcttaataa
     1861 attttccaaa atgagtaatt gctgtaagca aacttttctt ttattcgata aaatttttta
     1921 gatcgctgta gttaatgtac tgcaaaatta aaatgaatat ttcttcgaca tggattcccg
     1981 cttgcgcggg aatgacaagt atgggagaat tgaatcttat gcagcagata attttatgat
     2041 gcagatcgac aacatttttg tataacaaaa ctatttcaca aactctatca ttagattggt
     2101 tgaattttta aaatagttaa tttattttac agacatacct tttaaaacta attaacaacc
     2161 ggtgaaagat tttgacaaat tatgggatta tagtgatcct gcaggtacgg aaattaaatt
     2221 ccgtgagata cttgatgaag taaaaaattc taacgacaat tcagcttacc ttcagctgca
     2281 gactcagatc gccaggacgc tcggacttca gatgaaattt gatgatgctc ataagcttct
     2341 tgatgaagtc gaaaaagaac tttctgacaa agaagatcct gtcgcgcgag tccgttatct
     2401 gcttgaaaga ggaagagctt ttaattcctc taaacaaaaa gataaagccc gtgaactttt
     2461 cctgaaagca tatgaacttg caaaaaaaga aggcgaagat aactttgcag tggatgctgc
     2521 acacatgatg ggaatagtcg aacccggtga cgaatctttg cgatggaatg aaattgcaat
     2581 gaaagatgcc gaagcttccg atgtacctaa agcaaggagc tggctcggtt ctctctataa
     2641 taataccggc tggacatact ttgacatgaa ggattatgat aaagcgcttg ctgtatttac
     2701 aaaatgcaaa aactggtatg aggaaaaaga aagaccttta gaaattgcaa tagcaaaatg
     2761 gagcattgca aaaactttga ggatgatggg aaaaactgat gaagctttgg aaattcagct
     2821 cgcgcttctt aaagatattg aagacggaaa agccggaagc gacggatata tttatgaaga
     2881 attaacggaa tgttatcttt tgaaaaatga aaaaactaaa gcgtcagatt ttgcaggtaa
     2941 agcttacgaa cttttatcaa aagatatttg ggtagcagaa aatgaaaagg aaaaacttga
     3001 acgtttaaaa aatataagta taggttaatt ttatgaagat tggtcatatt gaaatttttg
     3061 taaaagatcc gatggtatca aaagattttt atgaaaaaat cctgggcttt acagtcactg
     3121 aagtacagca tgaaaaatat gtatggctga aactcggaaa gactcaaata cttttgcgtc
     3181 cgggaaaaaa tgatctgaag acaaatgaat ataaagacag caaagtagga attgtaattt
     3241 atactgatga ccattcaaaa actagatctg agcttgaagg caaaggactt gtcttctgcg
     3301 gtactgacgg ttctgattcc tgtctcactt ttaaagatcc tgacggaaac tggtttcagc
     3361 tggtaaatcc cagtcacgct taaaaatttt ttattaattt aaataaaata aaatgagcag
     3421 taaattatca ccggaagaaa ttaaaaaaac cgaagagcaa cttggtgaac tcgataaaga
     3481 gttagagtta ctaagaaaaa aacgtattga actgaattct aaactggcac agatggaagt
     3541 ttcagattat actatgaagg acagaaacgg aaacgaaaaa aaactttcgg aaatgtttgg
     3601 cgataaatct gaactgatac tgattcacaa catgggaaag tcatgttctt actgcacaat
     3661 gtgggctgac ggatttaaag acacatataa ggagatcgaa aagaaagcgg cctttgtact
     3721 tgtctgcccg gataaacctg aagtgcataa agaatttgca gaagctcatg gctggaatta
     3781 tccaacctac tcagcaaacg gaacggaatt catttacgat atgggatacg aaatcagaaa
     3841 agaagataag acatattact ggcccggcgt ttccgtcttt gagaaaaaag atgacggcag
     3901 cattttaaga gtgtcaaaag attatttcgg accgggagat ttttattgca atatctggca
     3961 tttccttgac ctgcttccat cagaagatat tactacggat tcatagttca gttataaatt
     4021 gaatctgaat caaaactgaa tctctattaa aaaatatttg atgtatttta tatcaaaatt
     4081 ttagtaatca tataaaatat aaaactatgt cacgaacagt tacagatgtt tttgcaaagc
     4141 aatatgatgt aatggtggaa tgggttaacg gtgttatcag cggacttgcc gatgaagact
     4201 ttaatcttga acttgccccc gggaaaaata acggcgtctg gttactcgga catttaatag
     4261 tctgtgatga tgatttttcg gaatatatgg gtaaaggtga tattctttat ccgcagtata
     4321 tagaactatt tggtcagcat acgaaagtac aaacacctga taaatatcct tctgtaaaag
     4381 aactgagaga aagctgggaa aaggtttgtg aaaaaaatgt aaatgtatat aagcaaataa
     4441 ctgatgaaga acttaaagaa gatcatgcac ttgttaaaaa tgcagaagat gattatttca
     4501 aaacaaaaga aaatgtaata atgcactggc agcttcatga aatgtatcat gcaggtcagc
     4561 ttgcagtgat tttatcgatc tgcggaaaaa gtaaatatta aactgtatat agaatcttga
     4621 caaaattcat ctcagcctgg aagcagtatt tatcttatcc tcaccgtaaa gctgaatttt
     4681 taattacaat tttactgctg ataattactc taactacatt cagcaggttt ttattattta
     4741 acgaagcaag acagggtgcg atactggacg atccgattct tagcctgttc aatgcaattg
     4801 atctgaatgc tcttttattt tttgcaatct attcatcttt gctgatagct ttattttttt
     4861 tcagttttga tcccgaagtt ttattaactg gtattcagac ttacatactt cttgtaatgt
     4921 tcagaatgtc tgcaatgtat ctgatcccgc tcgatccccc ggtcggatgt attgaccttc
     4981 aggatcctgt ggtttttgta atgggaacag ggaaaaagat tgtaaaggat ctgttctttt
     5041 caggacatac atcaacagct tttttacttt ttcagactgc aaagaataaa tatctgaaga
     5101 catattttct gctggcgact ttaacagtcg ggattgctgt cattcttcag aaggcacatt
     5161 atacaattga tgtttacgca ggacttatat tttcatttgc atcttatcag atagtaaaaa
     5221 agatcagagc taaatttccg cttgtaaaaa ataaatcagc ataatgaaag aaagaaagaa
     5281 attttcatcc ggtgtgatct gggaagacat agtcggatac agccgggcag taaagataga
     5341 tgacagaatt ttagtttcgg gaactacagc catagtagat ggaaagatcg ttggtaaaga
     5401 tgatatgtat ttacagacca gaacaattat tgaaaaaata gaatctgttt tgatacaggc
     5461 cggaagcagt ctggaagata ttatcagact aagaattttt gtaactgaca tcaccaaatg
     5521 ggaagaagca ggcaaagctc tcggggaaaa attcagaacc atcaagcctg ctcagacact
     5581 tgtaggaata agtgcgctgg tggatccgga tatgctggta gaaattgaag ctgaagctat
     5641 taccacttcc tgaaatgaaa taatattttc cctgaatata tatttaatta attttttact
     5701 atgcgaatta taaattatat ttcaatcaaa attggataat cctcagactg aaagtaaaac
     5761 aaatttatta tcaatattta aaggttatct cctggcatta tggggaattt tatttttgat
     5821 gatcgtcttt tttttcttcg gggataaaat attatctgaa tcatattact taaatacttc
     5881 cgttttttta actaatacat ttgatactaa tttttttgtt tactgcttgg taggattttt
     5941 tgctcaggta atagacggtt cgcttggtat ggcatatgga gtgagctcca cttcatttct
     6001 tttaagtaca ggagtatcgc ctgctgttgc aagcgcaagt gttcatgttg cagaagtttt
     6061 tacaaccggt gcctcaggat tttctcattg gaaattcggt aacatagaca aaaaattatt
     6121 tctgaaatta ttattacccg gatccattgg agctgcaatc ggagcgttct tactcacttc
     6181 aatagacggc aatgatataa aaccctttgt ttcaatttat cttggtgtaa tgggcataat
     6241 aattataatc aaagcattta aaaaagtaat cttgtttaaa gaccacggca gagtttcatt
     6301 acttgcactc atagggggat ttgttgataa tattggcggc ggaggctggg gacctgttgt
     6361 aaccacgact ctgatcagtt caggtggtat acccagatat acagttggtt cagtaaatgc
     6421 agcagagttc tttgtttctc tgacaggtgc tacagtgttt actattacaa ttggtattga
     6481 tcactggaat attatagccg gattaattgc aggaggattg atagcagctc ctgttggtgc
     6541 ttttttgtgc tcgaggatta atcccaaaat actgatgatc atagtgggag tactgattgt
     6601 gttcttaagt atcagaactg tttttttgtc tgtgatttaa atatcaacct gaatttgttc
     6661 agttgaaatt atcaggattt tatttcacat tagtaaatga atatgcagtg gatataaaat
     6721 ttaaatggat cattttgtaa aaacatattt tattttcgat attattttaa agtaagttta
     6781 tttttaatat ttcaattatt acaataattt aatcaaaagc aggaggattt aaaaatgtta
     6841 ccaccaataa attttaagaa atggatagaa gacaaccggc atctcatgaa gccaccggtc
     6901 ggaaataaat gtatttacga cggagatttt attgtgatgg ttgtaggcgg tccgaattca
     6961 agaaaggatt atcactttca ggacggacct gagttttttt atcagatgga aggcgatata
     7021 caggtaggga ttgtagaaga cggaaaagaa actatctatg acattaaaga aggagaaata
     7081 ttttatgtgc cgccgaagac gccgcactct ccgagaagac ctgctgatac agtcggactt
     7141 gtagtagaaa gaaaaagaac agaagaagag ctcgacggat ttcagtggta ttgcgagaac
     7201 tgtcatcaga agctccacga aaaatttttt aagctgactg acattgtctc gcaactaccg
     7261 ccgttactca aagaatttta tgattctgaa gaattaagga cctgtaagca ctgcggatca
     7321 gtaatgcagc caccagttaa gagcaattaa aaattgcgaa tttcaaattt caaataaagt
     7381 gctgtatttt tattagaatt tagtttgatg tttttttctt tgttgaaaat ttctaatttc
     7441 ttttgtcata atttattttt atgataataa taaaataagg gaggattata tgattagaga
     7501 gagcgtcata cataaaaaaa gttatagctt ttcaattaga attatacggc tatatcaatt
     7561 cttaataaaa gaacataaag aatattcgtt agcaaaacaa attcttagat gtggaacatc
     7621 aatcggcgca aatatagaag aagcaacagg tgggtatagt aggaaagaat ttttatccaa
     7681 gctctcaatt tcttatcgtg aagctcgtga aacattatat tggttaaggc tcttatttga
     7741 tactgactat ttaaataaag aaatgttcga ttcactattc aaagactgtg aagaaatttt
     7801 aagaatatta tcaaaaattc aattaacaac aaaagcatct ctaaatataa aatgaaaata
     7861 tttgtaatta gtaatttgaa atttgaaatt gtatgaagat cgatctgcac acgcatattc
     7921 taccggaaac ctggcctgac ctgaaagaaa agtacggcta cggcggattc attaatctcg
     7981 aacatcacaa gccctgttgt gctaagatga tgaaggacgg aaaatttttc cgggagatac
     8041 aaaataattg ctgggatgca aaggtccgca taaaagattg tgatgaaact gaagtcagca
     8101 tgcaggttct ttctactgtg ccgataatgt tcagctactg ggcgaaaccg cttgatacac
     8161 ttgacctttc aaaatatcta aatgatcaca ttgcgggagt tgtcaatgat tttcctgaca
     8221 gatttatcgg attgggtact ctgccgatgc aggcaacgga tcttgctgtg aaagaacttg
     8281 aaagatgcat taaagatctt ggacttgcag gagttgaaat cggctcacat atcaacgact
     8341 ggaatctgga tgatgaaaaa ctttttccgg tttatgaagc agcagaagat ctcggcgcgg
     8401 ctatcttcgt tcatccgtgg gatatgatgg gaaaagaccg tatgccgaaa tactggctgc
     8461 cgtggcttgt cggcatgccg gctgaaactt cacttgcgat ctgttcaatg atctttggtg
     8521 gtgtcttcga aaaatttaaa aagctaaaag tctgttttgc gcacggtggg ggctcgttcc
     8581 ctatgacgat cggcaggatc gaacatggct ttaacgtcag acctgatctt gttgcaattg
     8641 acaacaaaat aaatccgcgt gaatatcttg gaaaatttta tctcgactct cttgtacatg
     8701 ataaagccgc acttaaattt ttaattgatc tagtcggtga tgagaaaatt gttctcggtt
     8761 cggattatcc atttccgctg ggagagcatc atccggggaa aatgatagag tcaatgaatg
     8821 aacttgatca ggctacaaaa gataagttgt tatggaaaaa tgctgttgag tttttgggga
     8881 taaaagttaa aaactgataa ttaaaaattt tcgaattaaa accattataa attcagcagg
     8941 ctattgatct attttaatct ttacattcct tccaaactat agtctgaata atttaaaagt
     9001 actaactcaa aacgcattca aaaaaaacgg gttattgatc ctctcttcca tatccttttt
     9061 ataaaactct ttcttatccg gtacattttc catcagaaat tcataatatg ttttataata
     9121 ttccttcatc gcattcttgt ctgactttgc attttgttta aatggatacg cctgttcttc
     9181 ggcgagatga ttcagcacat aatattcaag tgcgcgtatt ctgtcatccg gctggtaagt
     9241 gttcacagat ccgcagctgt cgcattttag attgactgcc ataaatgagt atatctttat
     9301 cggtagctct gctgcgcatt gagtgcagcg gtgaagtttt ttttcgtcta tatgtctttt
     9361 cacattttca agaatttttc ttgcagcttt tccgtaagtt gtgacttcga aaattccaaa
     9421 ctcccattcc atccactgac gcagatcatc tgattttctg tgctgttcat ttatctgatc
     9481 ccaggacact ccgatcttct gcatttcact ttccatttta ggccaggttt cttcggcttt
     9541 ttttgaaagc tgatctacag tctgattatg caatcctgtt tttatattgt gaatcacaac
     9601 tgtgtcatac tgaagattgt cgatcacgtt ttgaagagga gcatcggact gctgcagaat
     9661 ttcatagtaa cggtctttca gctttgaaag aaaattatcc catctctcaa tcagtttctg
     9721 aaactcggga gaattattgt cactgttaaa aattgaagaa attatatttt tcattatgct
     9781 ttttcaaaaa gtttacttcc gcaaaaaaga caactgtcat actgcatttc ttcaagtctt
     9841 ggcgaagcgc agtttttaca tctcatagta tgatgctttg cattttgcat tccctcagaa
     9901 tatttattta gtgtagccga agcatttgag aaaatatcag acattccaga aaaaggtttt
     9961 atcttttgaa aatattcatt caccttttga tcaatattga atgcaattag ttcagattga
    10021 actttagtat ccgaagagcc tgaatacttc tcaatgatct tttcataaac tttacgcagt
    10081 tctatcggga atgcacctct ggtaattttg atttccgaat tcaaaagtac ctgtttatca
    10141 gaatccggta aaggagatac ttcatacgtc tgcatgaaat cattccattc aataccaccg
    10201 cctttttctg aatgccatgc tttttgaaat gcctgatcag tttcagattt gaaaccctct
    10261 gaagaattta attcactcat aaataagttc agttaaaatt caatatcgtc atttgcaccg
    10321 gtaccggagc cctgcatact ttcatatttc tttttatact cttccatgag ctgtgacatt
    10381 ttagccgcaa gtgaaaaatc cgacatcatt tttccgctcc agtatgaact cacattggaa
    10441 taatctgcaa ctgtcattcc gaaatccttc aatacatcct gcgcatccct tccctgtttt
    10501 ccgaggacat cctgcgcaac cattgattct acatattttt caaaagggat ggaagatgca
    10561 tcagtctgtg atccgctgcc aaggttgcct gcagcatttg tattaaatgc tgcggaatat
    10621 tttgtggcaa tagtaaatga agtatcattc ttcattcttg aaagccattc gttatttact
    10681 ctgtcccatt tcggcatatc agcaccgatg gattttatta cttcttctat tacaccaccc
    10741 gaagcaagtg atgcatttac gcctgcccat tgctcaagag agattccttc tacaggttca
    10801 agatttgcac tcattttttt ttatttaaat ttataatagg gtaaatataa gaattttaat
    10861 tttttgtttc taagaatttt tttatttcat ttgcatattc tgtccaatgc agtccaaatt
    10921 attttttcaa acagtttcct gcaattgttg gtgttccaaa aaatttgaat gaataataaa
    10981 aaatcgaagg atcaatgtta tcgcaatttt ttaggtcaca acaatgagat cttgttgttg
    11041 gtgatcaaaa agtttacata tgatttgtag taatgttcaa ctgtcattca ggcaaacttt
    11101 ttgaaaacac aaacaacggc ggaaatccag agcaatgtag tgtttataaa tatcattttt
    11161 tgtattttgc atttacggat ccttcctttt taagaagttc atttttccac gaaaataatt
    11221 cgtgctaact ctaaaataaa gttagccttt atgttaattt atcgacggcg aaaattatct
    11281 tttatccggt aaattttttt tacttcaaag actcaaagat tcaaaaatta acaaactatt
    11341 aaaaacatta atactaaatt aaatgacttt agagaatttt ttaaaagaat atgataaaga
    11401 gggtgttgtt gttcttctag aaggcaaacg tcaggtaaaa aaaggtgatg aaagaaaact
    11461 gcagcgactt ggacaacttt tgaaagaaag atctaactat atgatattca gaagcggaaa
    11521 tgctgaaggt tcggacagat atttttttgc aggagcttca tcagttgata ataaacggct
    11581 gcaggcaatt acgccttatg acggacattt taaaaacagt aattcagcct atgaaacttt
    11641 ttcgctggat tctattgatc ttgttaaaga gcctgagatc acatatttat caaagcggaa
    11701 taagaaaaca aagggattga ttgataaata tgttgatgga aacagagata aatattcaat
    11761 caaggcagca tacataatca gagacactgt aaaagttaca ggatacgaaa atatgaaacc
    11821 ggcttccttt ggaatttttt atgatgatct tgataatcca aaaagcggag ggacagggca
    11881 tacgatggat gtatgtgaag aaaaaaacat tccggtaatt gatcagagag tatggttcaa
    11941 atggcttgag tagattccgg tttattattt atatattttg ataaacttaa aaccatataa
    12001 ataatcaaat ttatgagtgc atcacgtttc tttctttttt caaaagttat tttgatctta
    12061 gtaatattta tttctttaca gagctgcagc agcgaagaca atacagtaaa tcagccgaca
    12121 accggtgaga gtaatcgaac tgattcctac gtcagttttt aaaccattta aatctttact
    12181 gcgacgcgac ggaagacttc agtatgagcc tggaacggtt tcagaatatt tcagtctgtt
    12241 gacttttaaa attcctattc ctatttttgt tcaattaaat taatcacctc agaatacatt
    12301 tctacatttt caaaatcata atctctgtat aaaaaaatat acctttgttt aagttgttga
    12361 aataatttat taaacaaatt ttccgatcga tatttattgt tgattggaga aaaacttttt
    12421 tattaaatta aaacctcagg aggttaaaat gatttacgga aagttttatg caggggcatt
    12481 tctttttttt gtttcattaa ttatttttca aagctgttct gatgatggtg ttgttgctcc
    12541 cttaaacgat gatgtttggg gaatagtcgt caatggcaat ggtaacccaa tcccggacct
    12601 cgttgtcaga atcggagatc aatccactgt taccgacatg aacggaagat ataacttata
    12661 tgatattcaa acaccttatg atgtgttgat aaaagatgaa agttcgagaa gtcagatgct
    12721 tgtcaaatct gtcacctccc gtgaaattct gataccggat tttatcacaa gctacaatcc
    12781 ggacttcaaa gcttatgtaa atgtaaaaat tccgtcttcc attattcagt ccggtaagaa
    12841 gggcaaaata attttcactg acggcagcgg tctcaactca acaactgata ttacaagcga
    12901 cagcacaact cttgaagtga atttaaaata caattcccaa accaaaggta aattaattgt
    12961 attaatttat tctgttgacg ctgccggcag ggtattcact tataataatt atgcaagtaa
    13021 aacaggtatt gatctaacta atggctctac ttattcattt tcttttgaca cgaacgatct
    13081 gagttttaat ccgcctgaaa gaattattaa tggaagtata aatattcctg caggatataa
    13141 ctcttcatca cagtattatt atttgagttt tgctgacaga aatatttcat tcaatgatcc
    13201 ttcgtgcaaa ttcgctgata ttaccggtga taattttaac attgttgctc cttatgatct
    13261 tcctgatgta tttactgtca tggtcaataa ttattcaaat aattccgata aatattctct
    13321 ggaaacattt gtaattcatg actatgaaaa ctctgtaaat ctatcgtcgc atattcccgt
    13381 agatctgatt tcaccggcaa ataatatatc cggaattacc tctgattccg aaatctcatt
    13441 ttctcaggga gatggtacag gagtttatgc ggtcagattt ataaaagacg gatatatata
    13501 ttctattatt acttccgcga cgtctttccc attttctgaa cttgaaaaat ttgatattgg
    13561 aaatattaaa aatgaacatt tcatatggac tgtacaaaaa tatggccaca cctcaggatt
    13621 gcgtgagtat ctgaatgtaa ttcccataat tgaagttaag tttctgacaa aatccgaaca
    13681 gtggatgttt caggctgcac cataaaataa caataattaa tcgcggcgga caaccctctc
    13741 ctccgtgatt tataaatcat aagaaacttt taaaacattt aaaaattcaa gtttcaaaca
    13801 gataaataat cttgaatatc ttatgatgtt tttgtaagtt catttcgttt caggatttta
    13861 catggattga aaaaataaaa tactttataa attcaatatg cattcagcat atctaattac
    13921 tcagctggaa aataatgcag acgtattcaa atcacttctt tctggaattt ctgtagaaat
    13981 gataaaatgg aaaacatctg aaatgaactg gtctctgctc gaaatcatct gccatcttta
    14041 tgatgaagag cgtgaagact tccgtgcaag gcttgcaaaa atattacttg aagatcatga
    14101 gtgggatccg atcgatatac agacaagggt ggtttcagga aagtattctg aaaaagattt
    14161 tactactgtg ttaaatgatt ttttaaaaga aagaaaatta tctatagagt ggctgaaaaa
    14221 tctggaagtg catgactgga atatcaaagc tgttcatcca aaaatgggag agttttctgc
    14281 tatacagatg ttagccaact ggttagcaca tgattatctt catttcagac aaatcgtcaa
    14341 gacaaaatat ctatatcttc aaaattcgat ttcaccggca gatcttggat atgcaggtga
    14401 attgtgattt tttaaaaatt acttcttatc ccacggattt gtagcccaga cagtcacttc
    14461 aggtatcatt cctctgtcgt ccatttccaa aagagatttt attgtatgag caatctcgac
    14521 gcttgttagt ttattggcaa cttcatccct ttcttttctt tccttatttg caaatgcagt
    14581 cgtgacttca ctcggattta cgagcattac tctgatattg tgagggcgaa gctcagcctg
    14641 ccagcattcg ttcattcctc ttaatgcaaa ttttgatgaa ctgtaaatag tgcctcttgc
    14701 aaatcctttg agtgctgcag tcgaggcgat gttgatgata tttccgtaat tctgtttttt
    14761 aaaaatgtca gcagcgtgtt ttgtcatcag tgctgcaccg aatacattca ctgaataaac
    14821 attctgaaaa gcttccatcg taagatcttc tacttcagga aattcaccta tgcctgcatt
    14881 atttacaaga cagtcaagtc caccgaattc cttcatcata atttcataag tcttttttat
    14941 atcatcttca tttgaaacgt ctgcatgaat cgccagtgaa ccagtttcct ctgctgtttt
    15001 atcaagcttg tctttatctc gacctgtaat tgcaactttt gctccgcttt gtgacagcag
    15061 tactgcagca gctttaccga tacctgaact cccgcctgta ataagtattc ttgagtcttt
    15121 tattttcatt ggtttttatt tatttaaagt ttgaaaattt gtgaccgttt tttaaattta
    15181 taagaaattc ataaatgaat tttatactgt tctccacatc atctttatga actgtctcaa
    15241 ctgtcgtatg catgtacttt gtcggaacag agatcagtgc tgatgctaca ccttctgccg
    15301 aatatgcaaa tgcatccgtg tcagtaccgg acgatcttga aactgcctca aactgaaaag
    15361 aaattttttt cttcttagct atatctatca gcatcttctg aagttcatta tgaatggaag
    15421 gtccataagt aagagtaggt ccttttccgc atgcaatgtc cccgtccttt atcttatcat
    15481 acaaaggtga ctgagtatca tgagtaacat ctgtaattat agcaacgtcc ggtttaattc
    15541 tacgagagat catctgagct cccataagtc cgacttcttc ctgaactgca ttaacaatat
    15601 acaaaccaaa ttttagtttc tgtttttttt ctttcagcat cctcgccact tcggcaataa
    15661 taaatccgcc gatcctgtta tccattgccc ttgcgacata ataattttta ttcaaagtca
    15721 taaactcatc ttcaaatgtt acaacagaac ctacatgaat tccaagtgct tcgacttctt
    15781 ttttcgattt acttccgcag tcaagaataa tgtttttctt ttcgggctga tctccttttt
    15841 tatctctgac atgaatcgcc ggccatccga atattgcttt tacaaatcct ttatccgtat
    15901 gaatattcac tctctttgac ggagcaatag tatgatccga acccccgttt tctctgacat
    15961 aaatatatcc atcgtcattt atgtaattta caaaccaaga gatctcatcg gcatgtgctt
    16021 cgactactac tttatattcg gcgtcgggat ttattacacc tactaccgtc ccgtaaacat
    16081 ctgaaaaatg tgtgtcgata tagggtgtta aatattccag ccacagcttt tgacccggag
    16141 attcaaatcc cacgggtgaa gaaatattaa gataattttt taagaactct aaagattctt
    16201 ttctcaaaac acttttattt tatttattaa taatttcagt tgaattttct ttaccaataa
    16261 ttattgtatc tgcaatatca ataaaaagac ctgtctctac aatacctgtg acctgtttca
    16321 actccatatc cattatcaga ggatcttcaa ttacatttaa atgcagatca aatatataat
    16381 ttccgctgtc cgttttataa atatttccgt ccttcaatct taattcagga ttacaatctt
    16441 taagatgttc gagtactctt ttatatccga atggaattac ttcaacaggc aatggaaatt
    16501 taccaagtgt attaacaagc ttagccgaat ctactatcca aatatttttt tcggaattaa
    16561 gtgctaatat cttttcaaaa agaagagcac cgccgcctcc tttgataccg ttgaatgaac
    16621 tgtcagcttc atctgctcca tcaatgttaa tgtttatcaa aggaacatca tcgataggta
    16681 ttacttcaat tcccaatgac tttgcaagtt cggtagatgc atttgaactg gatacagctt
    16741 ttactttcag accatctttt actaggtcac ctattttatt aattgcataa taaactgtcg
    16801 agcctgtgcc gagtccaact gtcattccgt cttttacaaa ttctacagct ttctctgctg
    16861 ctagtttttt ttcggcttct ttctgaatgt ctttcaattg atatgttaaa tttattggat
    16921 taagaactta acaaactcaa cttatttaat aaatctaaaa gacagtataa tttacgccaa
    16981 gttgaagacc ttctttgagc tgtgttttga gtgactgtga tttttcataa accaaaagta
    17041 catctaagct ggcagtcatg aaatcattta taacagcggt cagagtattg tcccagccca
    17101 catcccagac atcaagctga tcgaaggcag agaataatct cagtcttgta ctgaagagta
    17161 aattatttgc aactacaaaa tttgcatccg taacagattc catacctgtc tgaaatttaa
    17221 atccttctgt ctcgccgggt gtgttcggat catctgtgta ttgtctgtaa acatttgcaa
    17281 aagtttcctg aaaagccaga ccaaatctcg aagagaacat cggtctgtca tatgttaatc
    17341 cgatactctg cgtaatatat cccggatcaa agaaagctga cgtctgaatg atcggatcca
    17401 aactgtaatc ataaccattc agaattcctg atctgatctc attgctgatg tatggatcaa
    17461 gtgcccagcc gatcttacgg ataaggacat tttcaaaata agctgcattg tcatttgtct
    17521 gaaaactgcc gcttccagtc ttagtcgaac caagagtgaa gctcagatta tttttaaaga
    17581 gccacggctt gctgtaatag atcgcataaa aatcagaatt acctgtgatg gtaagaagat
    17641 tttcaccgcc ttttgtccag ttcgtaagcg ctacctgatt gactccaagc ccgatcgtcc
    17701 caagcggtgt ccagcctttt ttgtaatagg ttgtgtcagt attacgggtg cgtttgaatt
    17761 tggtggtatc gctttgtgaa aatgaaagtt cagtaaagat gaataaaaaa gcaaaaagga
    17821 gaatcaagtt tttcatgatt tatatttagt tttaaaaaac taatataaat tttttgaggg
    17881 aaagacttaa tgaattattt ggggatattg aaattattta ttttgaaatt tataaaaatc
    17941 ttctgaagtt tgtttcttca gaagtttaat ttcaatttga aaggtatctt gttcattcaa
    18001 aatttttttt aatataaaaa ttaatttctc ctttctaata atttaccgct ctccacagag
    18061 tcacgagatt tttgactctg cagaaagcgg aattagttat ttaatcagca tcattgactt
    18121 tgaagccgta aaatctcccg cagttaattt ataaaaataa actccacccg aaagatctga
    18181 tgcatcaaat ttatactcat aagttcctgc cggatactct ttgtttatca gtgaataaac
    18241 ttctttgcca agtgtattat aaatttttaa agtaacaaaa tttctgacag gtattgtaaa
    18301 tgaaattgaa gtcgaaggat taaatggatt cggataattc tgattaagat taaaattgcc
    18361 cggtatggaa tttatattgc tgatgccgag aattccatca atatcaaaag taaaacatcc
    18421 tctgccgtgt gtagctgcca ttaaaatttt cggaccttct ttatatttca aatcataaac
    18481 ttctacattc ggaaaatctg tattgaatga agtccagctg actccgtcat ttgtagatct
    18541 gaaaacgccg aggtcagttc ctacaaatat catttttgga ttagtctctt ttattacaat
    18601 tgagtttgta cctatgttcg gcagattggt tgaaatgctg aaccagtttg tgccgccatt
    18661 ggtagtttta taaacatgct tgtcagcatt tgcaccgcca aaagcagcat aacatatttc
    18721 atcatttgat ctgtccaccc agaagtctga tatatatgga aagccggtct gtgacgacca
    18781 gtttgctccg ttatttgtcg tgacatgaat tcttccgtta cttgtaccaa tataaatttt
    18841 atctgtatta gtattactga ttccaatcgc tgatacactg ccgcctatat ctccggtagt
    18901 agctatctga gtccagccgg tagaagtcga tgctgtctga actccagaag ttttccatac
    18961 atcagctctt ccaaacacaa cagtattatg atctcccgga gccatctcat acggattata
    19021 aaaaagtccg cctgtcgatc cgcttggagt tatatttgta aaacctgcac caaaatttgc
    19081 tgagcgttgt atcgaaccat tcacatattg accatataca aaattcgtat tcacaggatc
    19141 tacaattgta taacctccgt caccggtagt cctttgattc cagaataatc ctccgtccgt
    19201 acttgtctgc ttattgttat cctgtgtacc gccatacata aactgcggat ttgtcggatc
    19261 ataatctgca ctttgaaact gaagtgttga catgttcata ttcagatctg tccaggtctc
    19321 cgcatcatca ggtgaaatgt aaactccgcc atcagagcag caatatagaa cagaaccttt
    19381 ataagaaaga taatgaatat ctgcatgtgc aaaatccgat tgcgaacttg tagaccagac
    19441 cgtcatttta gtaagagtta caccgccatt tgtagatctg taaatatcaa gtccgccgac
    19501 tattacttta ttcggatcgc ccggaacgac agtcactgca ttgtcatacc atccctgtga
    19561 actcatgtaa ttagttgtcg aattctgatt tgtccagaga tcaccgccgt ttgtagttct
    19621 gtataatcct aacaatgatc cggtcgttga agctatgctt gcataaatat aattcggatt
    19681 gtcgggagag attgcaagct gagtccttct gccgtttgaa gtatttaatc cgtttgtcag
    19741 cagagtccag ttcgtaccgc cgtttgatgt tttaaaaatc tgtgactgta catttcctga
    19801 tgaagcataa agtatctgag gatttacggg atcgataatt aaagaagtcg aatttaaaaa
    19861 taactggttc gtccaggtga gacctgcatt tgttgattta aaaattccat ttattccagc
    19921 gagataaata ttatttgtat taacataatc tatcaccatc tttcctgtgc gtgtaccaat
    19981 ctgtgcactt gttgcaatct taaaccagct catacctgca tttgtggatt tataaactcc
    20041 gtcaccgtat tggctgtctg aggaataatt caattcacca gttccataat acagaatatc
    20101 aggattagaa ggatctaaaa taatatcccc gctggcaatt gaaccaagag tatctgtaag
    20161 cacagtccag ttcacaccgc cgtcagtggt cttccacact ccgccctgtg cagcagctat
    20221 ataaaaaatg ttagggttcg ctggatgaaa tgcaatcgag tttgttctgc ctgctataaa
    20281 attattgttt gtccttgaat aaaacatacc agtcggattt acactgatcc atccggtcag
    20341 agaagtcgga ctgttaatat ccattttgtc tcttgtcatc atttttttct gctgaacagc
    20401 ttttgcaaat gcatcaaaag gcaatacctg tccgggtccg cctgcacgtc tgtcagtaat
    20461 ataaaattct ctctgcaaaa ttgtctcact ggtttcacct tcttcatcct gcgcatatat
    20521 aacaggagtt gctaatgtga gtattgaaaa tagaatcagg aacagtttca taatttatag
    20581 tttaaatttt agattcgttg ggatagtcaa tatacttaaa atcattattt gaaaaaacga
    20641 ctgtattgaa ttaattccgc agatgtttta cctcaaaggc tctaagactc gaagtgggta
    20701 tgtttattat ttagaatata ttttatgggc tcttgagaat tgaaaaaaat gaatacttaa
    20761 ttttaaaata gtgtcatttt aaaaatttac cggcgtttcc aattttcttc ttaaaagatc
    20821 ttttgaatga attactgaaa ataatattca aattatgtta ttcaagtatt tttttttagt
    20881 cctgaatatt gttggaaaag attagatcct tatcttttaa ttcctgttca gaatttttaa
    20941 aattattata aatatcctca cgtctttctt caataattta atattattat ccacttaaat
    21001 tttggaggtt ttgacagcgg aaattttttt aaatgctttc gatttatttt ttactttttc
    21061 tattatatct ttcttttgtt caaatgtttt tccttcaaat tctttagcaa tttcttcttt
    21121 tacttttttg aaagaactct actgaatcaa aatccttatt aattttttta gttttcataa
    21181 aactgtgaat aaaagaaatt aattcaaaga ggcaaaacct tttacagatt ttacctcttt
    21241 gaaaaaatat attttaaaaa taattactgc ttaattttat ttttagtctt actatttgat
    21301 ttagctttcc cgttaccatt caaatgtatc ataggtgtca gcaaaggctt gtctttgaaa
    21361 tcaggaatta tgacaggttt cttttttagt tgatcaagtg cgatctcgat tgctttatca
    21421 agctgcggat ctcttccttt gacataatct tcaggagtga tctcaacttc gatatccgga
    21481 tcagtaccat aattttctac tttccagccg acatctttca tgtaagttgc atactcaggc
    21541 tgagtcgtca ttgtaccgtc tgcaagcatc agtcgcggat ttattcctac aactccgccc
    21601 catgtcctct ttccgacaag cgtaccgagt ttcatttgtt taaactgatg actgaaaata
    21661 tcaccgtctg aacctgcatg ttcatctgtc agagcaacca taggtccggc tacactgtga
    21721 ctaggataag ttccgacact gccatatctc gaaagatcat agccgtattg tttcctcgat
    21781 agcttctcta ataaaagctg tgatacgtga ccgcctctgt tattacggag atctacgaca
    21841 agtccgttca ttcctgcctc atgaatataa tatctgtgaa actctgaaaa tccgttgggt
    21901 cccatatcag gaatgtgcag atagccgagt ttaccttttg attttttatg aacatagtct
    21961 ttatttttct caacccactc tctgtatctt ggttcatatt cagatgcaat tgtttttaca
    22021 ttaaatgatc ttgtctctcc tgttttagaa ttttttacaa gtaagctgac aatattcttt
    22081 gcctgattta ctaaaagttc ttcaggtaaa atatttttac ttaatctgtc accgtttata
    22141 gcaatcagat agtcaccaac gctgacttca acaccgggtg cattcagagg agaatttttt
    22201 ttccaagaat cgccattcac aatgtgtgtg atcttatatg cattttcctt ttcatcaaat
    22261 tcataatccg caccaagttt tccaagtctg taagttggag aaggtttata gtcaccgccc
    22321 atttcataag catgagaagt gccaagctca ccctgcattt cccagattag atctgaaaat
    22381 tcagaacgtg aagctacttt atcaagaagc ggtaaatatc ttttataaac attatcccag
    22441 tttattcccg acatatcagg agtccagaaa tgttccttct gaagtctcca tgattccgaa
    22501 tacatttgct tccattcact gatcggatca atattaattt ttactcttga aagatcgagc
    22561 cagtcattct tccctcttcc atgtccactg taatcatcat cgtgatcacc atgatgatca
    22621 tgatgttttt ccgcaagcac ttttgaattt atataaagtt tcttttcctg cctgtaaata
    22681 agagtacttc cgtccattga aactctaaag ctttttatct tttttgctac atgttcattt
    22741 ttctgagtat gaaaatcaaa aagatcaagt gattcgtccg aaggatgctc gtcccagtac
    22801 cagtgatgca gtgcaccttt tatcggctct ctgctgtaaa gaagtccgtc ctttacagct
    22861 gcaatttgaa aataattttt ctcaggtaac ggaaatgaaa ttatcctttg atcaatttcg
    22921 tccagatcga tcagaacttc ttttgatttt ttcttattct cttttgcaac ttttttatct
    22981 ttattgttat cactatcctt ttctctgttc ttctcttttc cattattgcg ttcacgtgca
    23041 aaaagtttat catactcagg agtaaatgga gattgaatat ctttccttaa tgtgatcaga
    23101 taaggtctga caccaagcgt aaatccaagc tggaaaaaac ttgtgtcata agtaggatta
    23161 tattccctct gagataagaa ataaatatat cttccgtcag gatcaaagac aggctggtaa
    23221 tccctaaaac ctgcgggagt tatctgtgaa gattttcccg tctcaagatt atataaatac
    23281 agtgatgaaa gatgttctaa attagacata ctataaacaa tccattttcc gtcaggtgaa
    23341 aatgaaaaat cctctatcct ttcaaattta cttctggcaa tttttttgaa tgacatattt
    23401 tcagcatcta taataaacag ttcatgtctg ttatttgaaa ctgcgatctt catttctttt
    23461 ggagagactt tcagattggc aattataccg gctttgaaaa aatctaattt tttaacttct
    23521 gaatttcctt ccaatgaatt gtaaatttct atcgttccaa aatctgtttc atctgataca
    23581 gttacaacag atttattatc atacatccat tgagtaagtc tgtatctcac gccaagtttt
    23641 ttccctgcct gaagtacagg accttcccag tttgaaaaca taaatgattt tcctctagaa
    23701 gtaatggtcg tagattttcc gtcaggactg agattgtaag attccagata cttgcctgcg
    23761 tcggcaaact tcctctgcct ctgtatttgc ggactgtaat attctatatc aattttattc
    23821 tcagtatctg actcaatatt ataaacaaaa atatctgcac cactgtgata gacaatatta
    23881 tttccatcgg tcgttgcatt ccttacataa tattctgtgt ttttcgaaag agctttaatt
    23941 ccttttccat caatgtcaca tgaatacagt ctgccgattc cttcatgatc tgaaataaaa
    24001 taaattttat caccaaccca aagagggctt gcaagatttc cttttacttt agtgagaaat
    24061 ttttcaaact ttccgttgcc ttttttatct atccagaaaa cacctgccgt accgccttta
    24121 taccttttcc atcttgcagg atcgagtgta ttccttccta ttaaaactcc gccttttggt
    24181 ccgaatgcaa tattttgtcc tttaccataa ggaagcagtt caggcattcc gccgtcgaca
    24241 gagactttgt aaagcatgtt cattcctgta aatgcttgtt ttgtatcaga ggaaaaaatg
    24301 acatacttgc tgtcaggagt ccatcctttc acttttaaaa aaccaccgag ataagtgagt
    24361 cttttattca taccaccctg agacggaatg agataaacat cattctctcc ttcttcttta
    24421 ccggtgaagg caatatattt gccatccgga gaataatatg gattgattac ttcgcctgaa
    24481 tttgatgtga tcctgcgggc tatgccacct gatagattaa cttcccaaag atcatcttca
    24541 ctgacaaatg caactttgtc tccgctgaca gttggaaatc ttttataacc taaatcattc
    24601 attgtgtgct atttgaaatt aaattaaaga atatttataa cgtatcttcc ccaaaaaatg
    24661 tttttataac ataacttttt tgaaatttat tgtctatgaa tttttaatgt caatttcttt
    24721 taaattccca tatttattca tcagcataaa attttataat acctcaaagg ctcgaagtct
    24781 caaagatatt tatttaacta tgagccttaa agtcttggta tcaaatcaaa tgttaaaaca
    24841 ctatatgtga atatagtaga agtgaaaaaa ttaaattttt tatccttcaa tgatctgacc
    24901 ttcaaaggca attttaaaat ctttagaaat tatatattta ctttcttccg agtctcctct
    24961 gatcagtgga tttgtatgat ttaaatgaat gaaatgtaat ttactttttt ccttcaaaga
    25021 aagctcttca aactttttaa tactgtcgct gaccaaagga tgaggtatat tttttgactc
    25081 ataatttttg atctcttctt ttgtaaaaaa agtcccgtcg agaaatagcg cgtctgattt
    25141 tttaatgatc tttagaatgt ctttatccca aatctcccaa ctatcaatat caggaatgta
    25201 aattattttt ttagaattaa aatttatctg gtatccggct gtttctgaat actcatctct
    25261 gtgaagaact tcaaatggct gaactgctat cctttcatta agtacaattt catcatcaaa
    25321 ttttaattct ctcaattcaa tattacctga attttcaaga agtttccatg ggtgattatt
    25381 catcagaaaa tttttcatct caggcaatac atatacaggc agcattctta tacccatgat
    25441 ttcttttcca aaatgtatca gaccggtata gtgtcctgtg tgagcatgtg ttagaaatat
    25501 tcccttcagt ttacattctt tgaatttttc ctcagccata ttcatctgaa attttatatc
    25561 gggtgtggca tcgatgagcc aataactttc agactgcgga tcagcaattc caatacatgc
    25621 tgctgacctt tgaagagatt tatctttcca tgcatcttta cagcagtctt ttttacagcc
    25681 ggcatgcgga tatcctgcat cctgagcaat accgagaatt atgacagaag gattttccat
    25741 tctgtggtta gtttaaattt ttataagata gtaatttttg ggaaatgaat tctattaaaa
    25801 attggggagg tctgccgtgc tcttgtacta agattgtggt ggtcaaaaaa tttgcagcgt
    25861 tattaaatat aaaatgaaat catatattaa caaatttttt gaaacaccaa caatggatca
    25921 aattttaaat gaaagttaaa ttctaaacta agcagcagtt tctattacag cagatttagt
    25981 agtaggtaat ggaattttgt atcctctttt tttcatgtct tttatatgaa gttcgattcc
    26041 ttcttccata agttttaata aatttttttc agaatcagaa gtagcaatac atccgggaag
    26101 atcaggtatg taagcactat aactattttt tcctttttca attattatta aatattcttt
    26161 tttcatgata tgttatttaa aatttcagcc tgtttaaata tgctttttaa agtttttgga
    26221 tgaacatctt catttggttt tccagctatc gttacagttc cgtgtttaac aggatgatgg
    26281 tactgttgat gactccctgt ctgtctgacg aaatacgatc aatctttttt cagaattcct
    26341 ataacttctt taaatttcat tcggtctttt atctgttaca atcctttaaa gctaacacaa
    26401 aactaagaaa aatttacagg ctttacaata atataaatag gtttatatat aagtacagtg
    26461 ttcatgattt tgtttcagga cacttatttt ataatgcctc aaatttaatt tatttatttt
    26521 taaacgacat ttttcactat ttcaaaacga tcagtttttt tactgcttta aaatctcccg
    26581 aaacaagttc ataaaaataa acaccacttg acaatgaatg ctgctgacct ttgaagagat
    26641 ttatctttcc atgcatcttt acagcagtct tttttacagc cggcatgcgg atatcctgca
    26701 tcctgagcaa taccgagaat tatgacagaa ggattttcca ttctgtggtt agtttaaatt
    26761 tttataagat agtaattttt gggaaatgaa ttctattaaa aattggggag gtctgccgtg
    26821 ctcttgttcg aaggttgtcg gtggtcaaaa aatttgcagc gtttttaaat ataaaaggaa
    26881 attttatatc agcaaatttt ttaaaacact aacaacggct caatgattag aattttttgt
    26941 aatggatttt taaatacaaa ttctctgaat atactgagtt gtttggatca aacaattcat
    27001 taagccgaat cacaaaatcc agataaacat attcaaacaa gccgctattt ttaaaatcat
    27061 ctatgatgtt aacagcatca gcagaggaat cagcaaattg tatttccgct atataccaaa
    27121 tcgaaacttt agaaattgaa tcaagttttc ctccatgtaa attgatatac tcattaactt
    27181 cttttaggta agtactttca gcagctttat ctctttgtaa gtacaagatt aatttgttct
    27241 taaaatattc aaccaattct ccttcatact caactttcct gatttgttgt gtttgactat
    27301 ttactggcga gatattgagg gcattgaaaa ctataaaaag aaaaactaaa aaaattaatt
    27361 tgaatgatag attcattttt tgattttttg aaactgctta attataaaaa taaaccgtaa
    27421 tctttaaaga tccacagagt gataatgtaa taatatctat acagcttaat gataatgtat
    27481 tagtctgatt accatttgta taagttgcag ttctgaactc attatcaaat ggatctgctt
    27541 tggtgatttg atgtgaattg atgtgaattg atgtgaattg aagaaaaata aaaaaatcct
    27601 ttttatataa ttattgaata ttaatcaatg agattttaat aaacaagtag cgggaaagag
    27661 taactaaatt aattaaaaac tttccaaata tttataaaaa aggttttcaa atttaaattt
    27721 aaccgagaac aaatattcac tgttttatta ttaagcaggt acattattct tatcttcgcg
    27781 ttgccagaaa cgatgctgag caattgaatc aataaaatct tttataaagt ttttactgta
    27841 gccattgctg ttaataatta cacccatctc tgcgggattt ttcaattcag agttttcgtc
    27901 attcatttta ccaattatat tgcttgattt tattaattca attccgtgat tatcagcagc
    27961 tatagctttg caatgtttat atgcttcatt tataaaatgt actgcatctg catttttaga
    28021 taaagtatct gcactgtttt tgccggcggg gatataaacc gcatcaaata aaactgatgc
    28081 tgctgttaaa aaactctgat caacatttat ccctttgcca tttttattat ttaattttcc
    28141 aagtgaagga gcaataattt ttatttgcgc cccttcagtt tccagtgctt ttttcatttt
    28201 attaatagaa ctttcatcaa caccatcagc agccagaaaa gcgatttgac gtgttttgat
    28261 agaatttttt atggtgtttt ccatgcttaa tgcttccgac ttttgcactg attgtttcac
    28321 aaaatggggc tgaaattttt taggatcacc atctgcaggg atactatgat ttacaggttg
    28381 cattggaccg gaaggaagcg acatacctag accctctgct actgaagctg ctaatgtttt
    28441 atctacctgt gttaataaac caaccatgcg ttcacgaatt tctactgttt cgacttttcc
    28501 aagttcaaat ctgagagcat ttatgatatg atttttttcg tactgcacct gactattata
    28561 gaataatgcc gcctgactaa agtggtcaaa aaaactttta cttcgtccgc ggattttctt
    28621 cgcgtcgatt ttttcagcat aggatgtaaa acctccctca ctcatttttg cctgaaaagg
    28681 acagcctccg tttaatgaat tcggttcata actgacctgt cctttattta tcatctgacg
    28741 catatgtccg tcgcgctgat tattgtgtaa aggcgacaca ggacgattaa ttgggatttc
    28801 atgaaagtta ggacttccaa gacgggacaa ttgtgtatca gtatatgaaa ataaacgtcc
    28861 ctgtaaaaga ggatcatttg taaaatctat tccgggtaca agatgtcccg gatgaaatgc
    28921 aacctgttct gtttcagcaa aaaaattgtc cggattcctg ttgagagtca tctttcctat
    28981 tcttctaaca ggtattattt cttcaggaat aatttttgta gggtccagta aatcaaattc
    29041 aaacttatgt tcatcttctt caggaactat ctgaactccc aattcccatt cgggaaaagc
    29101 tccgttttct atagcttccc ataaatccct tctgtgaaaa tcagaatctt taccggaaat
    29161 attttgcgct tcatcccatg ctactgaatg aactccaagt aatggcttcc agtgaaattt
    29221 tacaaatact gatttacctt tttcattaat aaatctgaat gtatgaattc caaaaccttc
    29281 catcatacgc aggcttcgcg gaattgctct gtcactcatc acccacatta tcatatgcat
    29341 tgattcaggc attaaggaaa taaaatccca gaatgtatca tgagcagaag ctgcctgcgg
    29401 catttcatta tggggttcag gttttactgc atgtataaga tcaggaaatt taatggcatc
    29461 ctgtataaag aaaaccggca tattatttcc caccagatca aaatttcctt cattggtata
    29521 aaattttact gcaaatcctc tgacatccct tgcgagatca gttgatccgc gtgaacctgc
    29581 aacagttgag aatcttacaa aaaccggtgt cttttttccc ggatcctgta aaaatcctgc
    29641 tctggtgtat tttgacatag attcatacac ctgaaaatat ccgtgtgctg ctgatccgcg
    29701 cgcatgcaca atcctttccg ggatacgttc atgatcaaag tgtgtaattt tctctctgag
    29761 aataaaatct tcaagaagcg ttgaacctct ttcaccagat ttaagagagt tttgattatc
    29821 attgatcctc agaccctgat tagttgttaa aaattctccc gaaccatttt ctgtgttttg
    29881 tccaagatca tctgtttttt tattaccatt ttcattctta gcaggttttt tctttgccat
    29941 aataattttt tgtatttagt ttaagaatct gttttataac cttggtctca aaaattaaaa
    30001 aagcgttttg gggcagaagt ttttacacat ttcatttaga ctttatgatt tttgagaata
    30061 atttgtaact aaatatactt caaaagctgt gccatacctg attgtttatt tttaagcaat
    30121 tatacagcct tatgtaaatt gaatttatga catttttgac aaatctgaca ttttgtcaat
    30181 attattattc ttaaattaga atttagatca taatttttct cagcttttac atttatttta
    30241 gagttaaaaa tctgagttta attaaatact aattttttga atttttcaat aatcaaaaaa
    30301 tcagattgaa atcaaaactt cccgataccg gcacgactat tttttcagta atgtctgcac
    30361 ttgcaaataa acataatgca gtaaatctct cacagggctt tccggatttt cctgtcgatc
    30421 caattttatc tgaactcgtt tttaaagctt ctcgagacgg acataatcag tatgcgccaa
    30481 tgcccggatt gaagtcactc cgagacggaa tttcaaatat aatttctgaa atatatggag
    30541 ttcataaaga tcccgataaa gaaattactg taacttcagg agcgacagag gcactatttg
    30601 cagctataag tacagtagta aatccgggtg acgaagtgat catgttcgac ccgtcatatg
    30661 attcatatga accggcagta attttaaacg gaggaatatc tgtcagaata ccactgacat
    30721 ttccgtctta tactttagac aaagaaaaaa taatttctgc agttaataaa aatacaaaac
    30781 tcataatatt aaactctcca aataatccga caggtgcact catttcaaaa gaggatttta
    30841 actttatcaa agaaattgtt ttaaaaaatg atctatactt actaagtgat gaggtatatg
    30901 agcatattat ttttgacggg ctgaaacatt attctgtatt gcctgatgaa gaattattta
    30961 aacggtcatt tgtagtatct tcattcggca agacatttca catcaccggc tggaaggtcg
    31021 gatactgtac tgctcctgag gaattaactg acgagttccg caagatccac cagttcctga
    31081 ctttttctac atcgacacct tttcaatttg cgctgacaga atatataaaa gacattggca
    31141 agataacagc tctaaaaaac ttttatcagt cgaagcggga ttacttccgc gggctacttg
    31201 aaagaaacgg tttaaaaccg tttctatgca gcggcaccta cttccagctt atcgactact
    31261 cttccatctc tgacgaatac gacctcgact ttgctgcccg ccttactaaa gatttcggca
    31321 tcgcttccat tcccgtctct gtattttata aagacaaaac agataacaaa gtcctccgct
    31381 tctgctttgc aaaagaagat aaaactctgg atgaagctgc tgaatgtcta aagagaattt
    31441 tgagatgaat taaaaaataa atttaatttt cctctatcga atataactca agctcttcgc
    31501 tctttcccct taattttatc ttacccatgg actcagtttt atactcttct ttcagattca
    31561 tttttttaag caatgtctct gatatcagaa atgatttacc gaatccatta cattcatact
    31621 gaatacgaga agaagtattc actgtatctc cgtgatacac tatgtctttt tttatgtcgc
    31681 caatctcgcc cgttattact ttcccaaaat gcaaacctgc cttgaattcg ggaaacacat
    31741 caaacttctt catatactga tcttttttac cggctattct attcttaatt ttaaaaaaac
    31801 atttaataca gttaagatta tttataccgt ccttcatccg ccatgttatg actatttcat
    31861 caccgacata ttggtagatc ctgcctttac actcaagtat agaatctgtc atatcataaa
    31921 aaaaatcatt gaggagttca tggtttttct ctaatccgat tctttctgcg atcgtcgttg
    31981 atgacttcag atccagaaac ataaagataa gttcttcttc aagaggcagc tgatattttc
    32041 ccgtgacata attcaacagc acattctgcc ccataagcct gttgatctgt cttataaaat
    32101 taattgtaaa tatcattaat acaccgacag ccagcattac aaaaaaggat ttactctcta
    32161 tgaagtttgt gaatgtacca aactttttta ctttttcctg aaatgtatca ttatgttcca
    32221 atcccatatt gatctttaat ataagcaata atattccgat gaaaataaaa atatacagca
    32281 gacttctcaa aattactgaa gtggaaaatt taagtctcct gaattttttt tcaagaaaat
    32341 agatctcaag taccgaaatg atcaaaccga atattaagcc aaagataaaa tgatttatta
    32401 tagggattag aagaaacccc tgaattaaaa ctgcaaaggt gactgttgtt aaagatgtaa
    32461 cggttatgaa gatcagaacc gaatttatgt ttcgtttact ttgaacattt aaactcatat
    32521 tattttagga tagggaaaaa ttttttagag ttattaatca atttaatttt aaactcaggc
    32581 tttgtattaa ttaaaatata ttactattcc cagacatgaa atctcatacc gtaaggatca
    32641 gctatcattt tgcttttctc actatacacc tgtgtgactt tacatccttc tttctctaat
    32701 attttcaagg cttcatcgat actatccact ttgaattcaa aaaatgtatt tccgccttcg
    32761 ctgttctcca tataaaaatt aattccgtct tttacaaaat gagtttcttt ttctgaagtc
    32821 ttctcaattt tcattcccaa tacgttttca taaaatcctt ttgctttctc ataatctttt
    32881 acctgtattg caatatgcgg actgaatttg aaattcatat tatatgattt aatttaaaaa
    32941 attattataa gaatgatatt ttattttacc gctcgttccc aaattcccag actgtccgaa
    33001 aacaaaaatt tgagtttgat tacaaggttg actccaggac ccgaaggttc aaggaagctt
    33061 atttttaaac tttttaaact ttttaaaatt tttaaaattt gagccttaga gccttagagg
    33121 taattccacc gttttcggtc aatctgtcct gtttaggaac gtatccaaac aaatctattt
    33181 agtttgactc aaaatttcta aagctcaaag aagtttaatt taaatgtatt aaaacttcaa
    33241 ggtttggagg cttttaggca cacctaaaga ttagaaaatg tatctataaa aacaaacctg
    33301 agccgacaaa aaatttgtaa ccttcatctg attttgcaac cgttaatccg atgttaaaca
    33361 ttctgtccag ataagtgatc cagaccgatg tgccgaatgt actgtgccat cttttggatt
    33421 cctctcctgt caggaatact cttccggtac ctccgaatac agataatcct aacattccgg
    33481 gaacgagaat atttactctt gcaactgaca ttcttatttc tgattgagca actacgactg
    33541 catctcctgc aaatctttct cttgaaaatc ctttcaaaga atttgcacct cccagaaaca
    33601 ttgattcata gaatggataa tctccccata cttttcctgc tccggtcctg aatgcaaacg
    33661 tcaccccttt gactgtatct gttgaaatat aagctctgat atccacaccg gcttttccga
    33721 atccgaaatt gttatcgaaa attttcggcg tccagtttcc aagtgcctgc gcatagattc
    33781 ctttgtacgg ttccattgta ttatcacgcg tatcataatt aaatgaagta tttattccgg
    33841 caaagtttaa tctgcctatg ccgtatgtat ttggattttg tccgagcagt gtattctgat
    33901 catagctgac attcggcatt ttgtaaaaag gagtcagtgt taattccaga tctttcctga
    33961 aaggaaaata aaatgtagtc gccactttaa ataatccctg ttttacatta taatactttt
    34021 gatcctgaag gctgtctgta aatggtgtct catttccttc cccgaaatat cttgttatgg
    34081 ccagctgcgt ttgaataact tctaaattta tccgtttatc atttattata gaataaaaat
    34141 ctcctgagta cttaaaatta taactcccgg aattaaaagc atagcttcct gtaagcgaca
    34201 ttttatatac ataaggtttt gctctgaatc catatttgta atataccgga ccgcctccca
    34261 gctcaattcc attatccgaa tcataattaa atacaggtgt gaatctccat tcatttcccc
    34321 gatcttctac tggcggttca tttctatact gaatattatc ttttggtctg tcatctttgt
    34381 aataccggat tccgttatct ttatttcctt tgactgttaa attttcatta tctttaatca
    34441 ctctgatctc aatgccgctg ttgttttccc ctttgaattc taaaatgtct ttgccttcat
    34501 tcagatagat ccttatctca tctgtatatt ccgaatcaaa tattttttta aaaaacggct
    34561 cacctttctt ttcttttgtt tccttatcct gtttaaacat tttcacttcg atctttttat
    34621 cactaatatt atttatctcg atgaattccc gtttatcact tccataaata tctgccacag
    34681 aatttaataa ttcaaaatat tcatccgttg cttcaggaag ctgatctctt ctggatttta
    34741 tcatactgat aagatttttc ccttctaaac tgtaccattc agccggcatc tgctttacag
    34801 cattaactat tacttcgtct gttatcttac tctgaatata tcttgccaaa gaatcaaatt
    34861 gttttttcgt cggttgagta agaaattttc tgtcaatata tctgccgttc caggaaagat
    34921 catatatcct tggataattt tctccgtacc cttcgatctg tgttacatat tctcctacaa
    34981 aaaatggaac gataccgtca tataaacaaa acgcctgatc tctgtcccgc gggatcggct
    35041 tatagatcat cttaccgtct tcctcaaatc ctgcccacag ccactggtca gaatgcctgt
    35101 cccagtcacc gatcatcaga tcaaataatc ttgcttttag aaattcaata tcatccacct
    35161 gattatcatt atcattttct aattttttat aaagtttata tgaatttgaa attttatctg
    35221 attcggcaaa tcccttttca tcttcatctc cgctttcagg attttcttca aaagtcccga
    35281 gtacatttgc aaagtccttt ctgtattcac caagtcttgg atcatcaggc atatacacaa
    35341 ttattggtac ggcattgatt atcccgacct gattcagcat agatgcgaca ataattgatg
    35401 caagcggatt tgatgtgctg atctgatcct gcactgcatc tgcaaataca gtttcctgaa
    35461 gatcttttgg aagaatcatt ttagggtctt tgtcaatcga acggaattta taaacttttc
    35521 cgtcttctcc ttttaatctt aaggattttg tctgcaggcc gccgccgcgt ttatatggag
    35581 tcagaccgcc ggcaaatgta ttcaggttaa gaatgtttac atcaaaaggt gtcgtccaca
    35641 gatccctcca gtgcttgcca agaaatattt ctgaaaatcc gcttatgtca tatttttcac
    35701 ccgctacgac tctgatcttt cctgaaaatc cgctttgtga agtatcaggt tttgtctgtg
    35761 aaaaaatata ttcggggatt aaaagcagta aaaaaagtaa aacgatatat ttcaaaatac
    35821 tttttgttaa tttttaatta ttattttttt ctaaacaaat atgaataaat tttcaggagc
    35881 actgaatttt aaatcgttct tttttttata aaaaattaat ttattcaaaa aaaaaattta
    35941 attcactctt caaattttta tttattccgc agatgcaatt tttttcatca taccgccgaa
    36001 aataaaaccg tgaaatggca taactgcata ccagtaaatt cttccgagca gacccaatgg
    36061 tctgaatgtt gctgtctgat aaaatacatt gttttcaatt ttgaattcaa gccaggcttc
    36121 ccccggcaat ttcatttcag cgtaaagtaa aagtctcatc tctcttttat ccgcatatag
    36181 tacacgccag aaatccagag aatctcctgc attgatcagg ttagtattct ttctccccct
    36241 gcgaagaccc actccaccaa acaacttatc aatgaaccct ctgatctccc aaagccagtt
    36301 cccgtagtac cagccgttcc ttcctccaat gcaccagatc ttattcaggg tggcgtcaac
    36361 attttttact actgtctctc ttttatcaat gtaacaaccg aaagtcggta cattaatatg
    36421 cttcgatatt ccttcgccaa gaattctgct tgacaaagca tctttccagc ttgaaactac
    36481 ttccgcattc tggattttat taaatgccag caatattgat tcttcatatg taagtaattt
    36541 tattcctaat aattctctca gattattttc tttacatatt atttctactc ccatactttt
    36601 cactaaatta gttgccagag taaatgaagt ggaagtcaca aaatacaacc agtatgatga
    36661 aagtttcggt gtcatcaccg gaacagtaat tatctttctg atcagatttc taactttcgc
    36721 aaatcttaac aacatgtcct tataactcat tatttccgga ccgcctatat cataactttt
    36781 atcataagtt tcttcattaa gtaaaactcc tgtaagaaat tctacaacat ttctgcttgc
    36841 aatcggctgg gattttgtac tgagccattt cggagctatc attaccggca gcttttccac
    36901 cagatcacgg atgatctcaa atgaagcact gcctgaacct aatattatcc cggctctgag
    36961 tgtagtcaga tgaaaatctg aatccgacaa tattttttct acagcccttc tcgatcttaa
    37021 atgttttgat aattttttat cattaattat cccgctgaga tatatgacct gtttgacatt
    37081 tgttttttca attctatttc tgaaattctc agcggtgact ttttccagcg attcaaaatc
    37141 ttcatttaat gccgacattg aatgtatcag ataataagct gcatctatat cagcaggtat
    37201 cagacttact gactctttat taagaaaatt tacttcaatg acttttaatt ttccagtacc
    37261 gtatttatct gcatcaaatc tgttgacatc tcttacacag caaatcactt catgtccatt
    37321 ctgaattaat gcaggaagaa gtctctgtcc gatatatcct gtagctccgg taattaaaat
    37381 tctcatttct aaatttataa ttctgattga tcaccctcat ctgaccggta attaattcag
    37441 aaattaattt ttaatttcat tcccaaaaaa cacttcaacc gaaattattt tatccgcgct
    37501 aaatttgata tactccgtat tacggaattt cattccgctc atattacaat cataaattac
    37561 aaaagcctct tcacctgact caactattgt ttcgatcggg ataaaaggtt tttgagtatt
    37621 cataggccag cacttttcaa aatatgcagc cctgtctata tgatcatcct gaggactcga
    37681 aaaaatgaat tcttctgata atatattttc aattgtattt ttatcacctg tttcatatga
    37741 acctaaatac ttccggatta tttctgactt tgtcattttg aaatgagtaa gtttttttga
    37801 taaattttta gtttaagtaa aacaaattac aaaaatcaat accgtttttc aacagtctca
    37861 tatttataat caaaatttcg tttcttccca aactacccag tctgtccgtt tggaaacgaa
    37921 cttgaaattg aaactccgtt tcaaatatac cttctacaat ttatcactag caggcgactt
    37981 ttatttcttc tttatatatc cccctgattc ttcagacttc ctaaaggctg aattacattt
    38041 aagactcaaa gctaaaaatt ttaaatataa acccttttga gtcttagaga cttcgagaca
    38101 ggattaaact ttacagtaat taaaaaaaag tgcattaatt tatgtaatta ttttcattat
    38161 aaattactac ttttgtataa ttaaaaataa aaaaaaatga aaaaaataat tttagtggtc
    38221 ttcgcaattg caatattcag taatataaat gcacaagaca taaaagaaac tgatgtacct
    38281 gaagctgtaa aaactgcatt ctatgcttct tatccggatg ccgataatgt agaatgggaa
    38341 atacaaggcg cgtattttga aggcggaata aaggttaatg acatggtaac atctgtttta
    38401 ttagatgcaa acggatttct cgtacagaca gaatcccaga tccctgcatc cgatctgccg
    38461 gacggtgtaa aggattacat ttcaaaaaat cttaacaatg cacaaataga agaagctgct
    38521 aaaataactg agccaacggg aaaagttacc tacatggcag aagtaaaaaa cagtgaatat
    38581 atgttcgatc cggaaggaaa acttttgtat aatttacagg gagattctga cagcggtgaa
    38641 aaagatagtg actagaatgt ttttaatttg aattaccatc cagctttttg taatactctg
    38701 aaaatttttc aagatcagaa gattcaaaca gatcataaat taaataatgt aaattttcag
    38761 gtgtttcctc ttttaaatac ctgataaatt catgagttgt agtatatggc ggaccttgaa
    38821 atttattttt ttcaagaaag gatctgagta ttcggttcag attatcttct cctataaatt
    38881 cactcaaaat ataaaacgca atcgaaccct taccatatga aatgtaagtc tgctcaggac
    38941 ttacatacat cagcggtaat tctttatcaa cttcttccgc tctgcctttc agataacgat
    39001 catactgaat tttcagaaaa cccattgcct tttctcttcc aaattttctt tcgtaaattt
    39061 ttgccgtaat gtattccgta atgctctcgg taatcataag tgcacctgct gcatctgcag
    39121 gtatgatctg atttccccac cactgatgag tgagttcatg agcagccgta taaaaggaaa
    39181 tgtcagtgtt tttttcaccg tcctctcccg tatcatttat aaatcttatc tcagagatct
    39241 gaattgtgtt acctgatgta gttgcatagc tgccttcaga tcttggaaat tctacgatcg
    39301 aaatattact gtgctgatat tctccaaaat attctgtgtt ataatcaaac gcagatttaa
    39361 tgccgtctgc aaaattattt atgttattgc tgtgatcttt ataataaaaa atgctgagct
    39421 cttttccttt gtatgattct tgataaatat taaactctcc tgaatttatt cctaaaacaa
    39481 atttgatatt cccttcagat ttgtaatgaa aataatttct gccgttttgc tctctcttat
    39541 ccatcagtat tccaggcatt ataacaatct gatctttact cgtactcaca gttgcttcga
    39601 tatttataag gtcagaatcg tatctctgat aatgatttaa gtgcgaaata ctgtcttccg
    39661 gatttaattt attttcattt ttaagattta taaatccgat atgaggaaat atgttactct
    39721 ttataaaagt gccgtcttca agtacatttg agttttgtgt tagaattgtg tttgaaatat
    39781 ttttaatgct gaaatataat tttatagaat cttttggttc tattccttta cttaatttat
    39841 atactgaaaa tttcagaaca ctgtcataag cttcaagttc agttccttct gaaaaagtat
    39901 aagaagtgtt ttcatcatag ccgcttttta aaagtagttt gtcgattaca taatttgttt
    39961 tgtttaccag tatataaaat ccttctgctg taaatgactt ttcatccgga aaaatatcca
    40021 tattaaaatt cagaagtaca atcctcggct gagggaaaga atcatattta ccaaattcat
    40081 tttgaaaatt taacatgtac tcagatttct gtttttctgt cagtataatt tcagactcat
    40141 ttttcttttc actgtaaata taaatcccac tcaaaataaa taatgccatt aatcctgtca
    40201 gtggcataag ttttttccca taaaatctta aattaacatt tttgattctt tcagaaatta
    40261 aatcagcttt taatcttttg ttaaaaatta ttgtaagatt gatcaatacc atacaaaaca
    40321 gaacccagta taattttgaa acaaagtaag gatacaccga atgtccaaat cccgatatct
    40381 ctgaatattt cagaaaaaaa tccggttcag gattcctgtt gaattttaat accaatgaac
    40441 ctgcatctcc aataggcaca ttgtctaatc caagaaaaat taaaactaat attatgagtc
    40501 cggtaaatga atttgttact aaggtttgaa taaacaatgc caatattgcc cagatcacaa
    40561 aaccgataag atgaattcca aataaagtgt aaatataatt accgatatcg aaattgtaat
    40621 acccgctgta gatctgtatc agaatacctg agatcatcat aatcaggagc atcagaatct
    40681 gaattttgat cagtgcagtc agctttgaga aatacaaaac aaaatcaggt gccggtgtca
    40741 tgcactctaa ttctttcatg cctgtacttt cagatctgtt gatcagtaat cctgaataaa
    40801 gaaatgtgag aaaaattatt agcagtgaat aaaataaaaa cggaatactc attattgtcc
    40861 atgtcacagg aagtaatttt acacctgtgt cagtatttcc tttcaggata agtacgacta
    40921 tgaagacagc accgattaat gctaatattc tgaatgacag atttcttgtt atgtaattga
    40981 aatcatattg agatagtctc catattgatc ttatattttg gctgaaagta taattaaatt
    41041 ttatgtcaag tgatcgatat gaagtttttg actcggataa taattccttt tttttaaaaa
    41101 aggatctctc ttttactttc tctgtcacag tgaatgaaaa ttttttataa gtataacata
    41161 agaccaacga tgctattgtc atccagaata ttctgttaat taaatattca aatccgaaag
    41221 gtatagatat acttgcaagt tcagatgcag ataaatactt aattttattc tcaaaataaa
    41281 tttcaccaaa tggatctgcc gtgatttcag gaaatccgaa tcctgtactg aacatgatac
    41341 cgctgatcaa tttagtaagc agtaataaaa ttataactgc cgggaaacct gaatagatgt
    41401 tctttgtcct taaaactact gtaaagacaa ttactgtaac caaaaataaa ttgggaagta
    41461 ggtaaaggaa aaaagtctgc agatatactg ataagtcaaa tccaattacc tgatcggaat
    41521 ttattccggg taataataat cctaaagcca ttccggacat taccaatatg gaaataaata
    41581 taactgttat aaaaccatta gtgaattttc ctgatagata atctgatttt tttaccggat
    41641 aggaaaagag aaacctgttg gttttgctga gataatctct gctgatcacc gttccgatta
    41701 taaccggaat cagcagcaga aataatttat taaaaaatat tgctgtagat aaaatatcta
    41761 ttggtgaatt tgctataccg gtacttcctg aaccttcgcc gaatattccc gcagcacctg
    41821 ataatgaaat gagagctgcc aagaaaaaca caaatgagta aaaataaaat gacagacttt
    41881 tcagccagag tcttaactca aatttaataa ttggaataaa cattagctca ctgtgtgctt
    41941 gtcattttca agtaccgtaa agtaaatgtc ttccagatca ggatcagcat caataaattc
    42001 tgctgaaggt ttctcttctg aatatacttt tacactcaga gaattgtctt cgttaaattt
    42061 agatgaaata actctgtgca atcttgtata ttcttccagc tcatttttat caatattaat
    42121 tacccagatc ttttgatcta attctttcac tgctgctgtt ggagaacctg ttttcaaaat
    42181 tcttcctttg ttaattattg ccatgtcatt acacagatct tttatatcat ctacaatatg
    42241 tgtagaaaaa ataattatgt ttgtgctcgc aatttcacgc agcagattca gaaaacgttt
    42301 tctctcagcg ggatccagtc ctgacgttgg ttcatccact attataattt tcggatcgtt
    42361 caacagtatc tgagcaattc caaatctttg tttcatccct cctgaatatt tactgacatg
    42421 catttttctg acatcaagca ggtttgtcat ttccagaact ttatccgtta tcatttttct
    42481 gtctcttgaa gatgatatac cttttaaaat agcaaagtag tcaagcagtt catctgcatt
    42541 cataccctga taaatcccaa attcctgagg cagataacca agtagttttc tgtaactcat
    42601 cggatcttca agtacattta tctcatcaaa atatatttct ccttcatcag gcagttgaac
    42661 tgttgcagcg atcctcatca gagtagattt accggcaccg tttggtccca gcagtccgaa
    42721 cattccctga ccgatttcaa tatttatatt gtcgagagct ttatgtgatc tttcgtagga
    42781 cttgcttaga ttattgatag aaaacttcaa taggaattta atatttattt aatgtacttt
    42841 ttttaaaaac ccttttttga cccgggataa ctgatcaggt gaaaaatata cggaatgatc
    42901 cagattttag tttaaatcgg catttaatta tgattgaata tgaattttaa caactactga
    42961 caggaactgt ttatttatgc aaacaaaagt aaaatttatt ttttatagat tttcagatct
    43021 ctgaatttaa aaaaggagaa agtcggagaa gaagcagtta ttctgtatat aaaaaaatca
    43081 tttacttttg gagaagctat tgtagtatct atatattttt ctgttggagt tatattcgca
    43141 gaactaaaat taaaaatatt aatttctgca ggaccctgat tataaaatat tttcagagga
    43201 gccggatcaa agttattttc tcccgaatag taaaaagtta atcttacact gtctctgtca
    43261 gaaaaattaa gtgtactgcc tgtaatactc tgagacatgg atgatgatcc tgaagatact
    43321 ccgacagaat cacctgacac ttcagccagt aatacttcgc ccgttgccgg tggatcaact
    43381 gaattatcct cactgctgca gcctataaaa gagataatcg atacaaagat tatgatcacc
    43441 ggtaaaaaaa gatccgttaa ttttttacac atcagaatat tttttatttg atttagaagt
    43501 atctcaattt ataaaaatta tattatatat tctatacttc aaaactttca gaacaagctc
    43561 tcttatttaa aaattatttt ttccggaata ataatgtaag tattctcgga atgccggtac
    43621 ggtcttcttt gtcaaaatat tcatttactt ccgaaagtat gaaaccgttc ttttttgcgg
    43681 agtttgtgaa atcagatatg ttgtgatcaa aacatgttac tgtctgaatg cctttttctg
    43741 tttcgaatct tgctttggtt cctgtatatt gtttgaaagg atgaagctct ccgatataga
    43801 ctttaccttc attggataaa gcttttgaaa cttcaaaaaa aatatgattc agatcttcga
    43861 tatgctcaag tacgagacta aaactgatga gatcaaattg ctcttttaca aaaatccatt
    43921 cattattaat atcagcctga ataaaactga ctttatccga caggatcttt tcttttgctt
    43981 ttgcgatcat ctcgggggaa atatcaaccg cagttaattt atctgaaatt gtcaaaagcc
    44041 attcagaatt tttacctgtg ccgcagccaa tctccagaca atttttaaaa ctcataccgg
    44101 caagttcttt tctaattgta ataccttcga gatctctcgt tttatttata tttgtatcat
    44161 actgctcaga ccatgatctg tatgcgtctt ttggttccat caaaaaaaaa tgtaattgaa
    44221 aaataatgta aaaattaaaa gaatatttta tctcgaaaaa tgttatccgt aaatatcttc
    44281 aagctctaat ttctcaatcc gcatcctgat tgcattgagt gtccttccaa aatgttttgc
    44341 tatttttttt acattagccc cttcacaata cattactgta agttcatcat ccagcttctc
    44401 tgtccatgga gtataagcag atttaaattt ctctctgacc ttatctgcgc tgtaaacttt
    44461 acttttttta cttccgctga aatcaggctt ttcataagat atctttgaat aattcttatt
    44521 gtctttattt ccggatacag gctcgccgat aattttcttt atcctttgtg aatccggaaa
    44581 accttttggg agaagtgtgt caggtatctc aatgcagttc tttgtcctcg ttactgccac
    44641 ataaagaagg ttgatctctt cattaatttt cgataatccg gaaccggttt tttttatttc
    44701 ctcaatctgc ttttcaagtt tttcttcatt tataaaatcg ttcactatct gaacagtatc
    44761 atactccatt cccttacatc tgtgaactgt tgaaaatatc acttcagctt tttccttttt
    44821 atcatcagac acatgcagct ctttgatctt ttttaagatc tgcggaatgt catttccata
    44881 ttccttaacg atctctacaa gcatagagag ctgagagtct ccggttttgt taatataatt
    44941 ctgaagttca ctcagagacc tcatctgttt tataagttta tcttttataa gtttatgttt
    45001 accattataa agatttaata catcataaag tgacgcacct tcttccgaat aagtataaga
    45061 attaaaatta ccttcaaagt aaagactcct gattttttta tttcctgtta ctatttcaat
    45121 tgcttttacc agaagaccaa gattagtcct gccaataaca gcgctagttc ctgattgagt
    45181 agaaagattt tttcctgaaa tgggacttgg tttgaattca gcaacctgac ttttcatttt
    45241 taaaatttcc attgcaagat cggcaatatc ctgactgaaa cgaaaactgt ttgtaagact
    45301 gaacgttttg aattcagctt tctcaagaga gttcacggca aatctccatc cgtagatctg
    45361 ctgatgagta tcgcctacaa ttactttcac tgcgtcctgt ttcaaaaata cgtcgagcat
    45421 tgccgccgat gcatcctgcc cttcatcaaa aagtatataa tcataattaa gttttggatt
    45481 taacaactga aatttcttta aataaaaatc atgagtgatc tcaatttcac cgctgttcat
    45541 tttacttaaa aaaagtcttg tctgattttc aatgtaatca taatgctttg agacaaatgc
    45601 atttgccctg ttgtcggaaa cgacatctgc ataattcagc tcactgactt tttgtttatc
    45661 gctgttacaa aaatatgaaa tgaatttact tacatgatta gcaacgatat gctcaccgtg
    45721 cttttcacct actccgctga gatgcagaat ttcacatatc tcatgcgact tgtatccttt
    45781 tgcgctcact ctgtatccgt gtccatacac aacatgtccg tacgcgagtg aatgcgccgt
    45841 ttcaactttc acattgtata attttttagc tgcaaatttt ttaagagctt ctgtctttac
    45901 tgatctatta aaagcgagat aaagtatttt gctgttttca gggcgggctt ttgcatactc
    45961 tattattgta gtggtcttac cggatccggc gactgcgttg atcttaatgt taccttcgga
    46021 actaatgatc tccttttgtt cttctgttaa tttcatatct tcttcattaa ttattaattt
    46081 ttctaaatat attcatcact gcttttatac tatacatttt ttcaaagtta gaaattattt
    46141 cttaaattcc aataccgcat caagttaatt attacataaa aaaatgtcaa agcagtattt
    46201 atctgaaaat tttatggata tcaaccaacg aatttggtgt tccaaaatgt ttataattca
    46261 aaacctggtt attcctgctc tattcaaaaa aactttttgt tcaccaacat ctgtgtcgaa
    46321 aaattttcat tactaagaat tcttttaccc tcataattta gattcaaata tgagaatttt
    46381 agtagattga tataaaagac taataatgtt aatataaaat ccgataacaa taataatttt
    46441 ttactgacca cttgaaatat tttataacga tcataatatt aacagcaact ttttattaca
    46501 gctgcagcaa tgacagcgtt caagatccaa atgctataac agaagattat acatcaatac
    46561 tgaatacgga taaattcgga aacatactcg gcggagatac tactgactgg tgtaaagata
    46621 cctgctcaaa tgcacttcag ccgtgcgatg cattttctcc ggcatatcca aatcccgtga
    46681 atgatacttt atttttcaga ttcagtgtaa gtagtaattt gtcaaattta aaattgtatt
    46741 ttttaaactc ttcatctgat acactctttt tagcaaatgg tgtttatgat gcaggacatt
    46801 atgtgctgtt tactacagca tctattttag gatattccaa tgtttataaa agagcatatt
    46861 tgaagatcaa tgatttttcc tgttacggtg acattaaatt taattgaaat tatcacttcc
    46921 tataacctcc cttattattt tccaaaaaaa tacttcttct gtaatgcgat gatctaaaaa
    46981 gaacatccat gagcagtata cttaaaacaa aacatctaca aaggaggtct ataaatgatt
    47041 ttcttattcc taaaaaacga aatgcaaatt ctcaattaat attaaacatt tgtgtcttgg
    47101 tgccttggtg gtaaaaaaac gttataaatc cggcaattta tttcttttca taatgctctc
    47161 tcaccaatgc cgccatttca ttttcagtca tcttgtcagc attctttaaa gtaatcgatt
    47221 ttttcgaaaa ttttttattc tttgaaagta aattattaag ctctgatttt gcaagtgtcg
    47281 gtccgaacaa taatatggaa tcatatttca gaagtctctt ttcaagatca ttaaaataag
    47341 atgctacttc attctgtttt ttctgatgca gtttaaattc agtattcgaa aaatactttt
    47401 taccaaatct tgtctcaaca ctactgctgt cttcaccggg aattcttaca tgtctcttat
    47461 aaggcgaatg gacagttgtt atctctgatc cgctttccat tatttctatt aattttgctt
    47521 ttgaatggtc tagccaaact cctattttct tttccatttt tactcctttt tataaaaagt
    47581 taattatctg taaactgttt ttaaatgtta aaatttttaa aaataatata tttgaaaact
    47641 aagtaacatc agaggtaaat gttttataca attttttttc tattaatttg attaaattat
    47701 ttttataaaa aaatttcccc cgataaaaaa tcgatctgta ttttaaataa agacatttta
    47761 taaaatgaca ggtacaaata aaagtacttc cgttcagaag tatgttttca ttacagcagc
    47821 agttgtactg ggaatacttt cactcatcgc gccaatatgg ccggatggac atattgagcc
    47881 gcgtgtcgga ggattgctga tagttgcagg aatcctcgaa atatttcacg gtttcagaag
    47941 atctaccgac gaaaaaagaa aggctgcctg gttcagctcg gctgttacaa ttacatttgg
    48001 tattctactt atcaatgcat ctctgttaat tagtacagca ctgctgattt ttattgttat
    48061 cacattttgt atcgacggga tcagatacgg tgcggaagct tttaaaaatt ttaaaaataa
    48121 gaaagattat ttgtatgatg tgctggctat gatcggaaat ttttccgtca tcgcagttat
    48181 attatttaca aaaaattttg gagcggactg gacaattgca atagtaggcg catggaggat
    48241 cttcggaact gcattaaata tttatcacgc aaaagaaggt aactacagat caagcggtga
    48301 agatgtaata aaaagtctgc accttcctga tacccgttcc gtaaaatctg ccgttagtaa
    48361 aattcagtct gaagaagatg ccagatatcc cgctgacaaa tcatggataa tagtttttct
    48421 tttactgctt tttttaattc atctcggaag gatggggttt gataaaactt cactgggaat
    48481 attctcacct gccgttgctc tctttggaga tatagtcgtt gcattgatta tcacattcgc
    48541 tatcatcacg ccgatcagag ctttattaaa aaaattgact ataccggtta caagaaagtt
    48601 atggctatgg gtggacaaag taccgtctga tcagagaaag aaattcggac cgagaaaaat
    48661 agtaaatgca tatcttgaac accgacttag agtttccatc cgcttacgta atgcaggata
    48721 ttcctttaaa tctgctttca tgacaggaat gaaaacggga cttccttatg cagcgatgct
    48781 tgctgccgta atacctgtat ttggaatgag ctggtatttt gatactgaga actgggctgc
    48841 aggagtatgg gacagctggg ctgcagcgcg gacagataac tggagaatgg caatcacaag
    48901 atctgtcgga gaacctccgg gtccggaagc atttttgctg catcccgaag gggtgaatgg
    48961 aaattcagat ttttcattta tagtaatcgg cgatccgggt gaaggtgatg cctcacagct
    49021 ttctctcaaa gatcagatac agatagtatc agaaaaaccc gatgtgaaat ttctagtgat
    49081 ctcaactgac attgtatatc cgtcgggtga aatgaaggat tatgaaaata aattctggct
    49141 gccgatgaaa ggaatttata aacctgtgta tgcaattccc ggaaatcacg actggtttga
    49201 tgcactcgag ggattctctg cgacattctt cgaacctcag gcagctttcg atggaatgaa
    49261 agcccgcgta actgccgatc ttaaaatttc tgcatctaca gatgatcaca taaacggact
    49321 tataaaagaa gctgaccgtc tcagaaataa ttacggagta ccgacaggtt ttcagaggtc
    49381 accttatttt cagatacaga cagataagtt tgcactcatt actatagaga ctggaatcct
    49441 gagaaggata gatgaagatc agatgacatg gctgaaacag gcactcgaag cttcaaaagg
    49501 gaaatacatc atggttctga tagggcatcc attttatgcg gtcggagaat atatgggtga
    49561 tcttaatcct gactttaaag acattcatca gctattaagg caatacaaag tcaatctcgt
    49621 aatgggaggc gatactcacg atctggaata ttacgtcgaa cgcggatatg atgatgatcc
    49681 cgattcaaaa atgattcact ttgtaaatgg cggaggcggt gcatatctca gcatcggcgc
    49741 cgctctcaaa ccaaaagatg aaatgcccga aaaggaatgg gcgatttatc ctgcgacagt
    49801 tccactttta atgaagatag aagaaaataa taatatactg aaagaacccg catggtactg
    49861 gacaaaaaat ctcaacggct ggcctttcac gcctgaattc ctttcggcag cattcgacta
    49921 taataattca ccgttctttc aaagcttctt tcagataaag gtcgagccgt caaatggcgt
    49981 catcagattc attccctacg gagttaatgg tcagcttaaa tggaaagaca tcgaaacttc
    50041 aggtaatgta agacctacag ataaaactcc cgaagacggg gcggagtggg tgtatgcttt
    50101 ggttgggaat tgaatgggat tgtaaagatg taatgaattt ttaacaatct aatttaaatc
    50161 acaagactga taatttgata ctttttcaaa atagattttt ataaaattat tccggaaata
    50221 aataaatcgg aattaattta attttatgaa atatgctata ataatagaga caggaaaaat
    50281 caaatacagt acatacatat atgaactgca gggctgtatc atcacagaaa aatttagaag
    50341 agttaaaata taatatgaaa gagacaataa attttcacat cagggaatta agataagatg
    50401 gaattgaaat tcctttgtca agttcgattt cagatactgt tgaagttggt ctttaatgtc
    50461 tgcaaaatat ttaatctttc cttaatattc tctccgctaa tgacctaata aacacagcaa
    50521 acattaacaa ctccaatcca aaatttaaat ctttcaatat taatttttaa accagataaa
    50581 aaatgaaacc tgatctaaac agatatattc aattgctgga gaaggctccg gttgtattat
    50641 cagatttgct taaagatctt cctaatgaac tgacacgatc taacgaaggt gaaaatacct
    50701 ggagtccttt tgaaattgtc agacatttga tagagggaga gaaaacagac tggatgatta
    50761 gaattaagat cattctttcg gacaatgaag acagatcttt cgagagtttt gaccgctttg
    50821 cacagatcac tgatagcaaa gataaatcaa tagaagagct tctggatata ttcagacatt
    50881 tacgaaaaga taatctgaga gtattaagtg aactagatcc gggtgaaaag gactgggaaa
    50941 gggagggaat tcatcctgat tttaataaag tgactctttc ggaattattg tcagcctggg
    51001 tagttcatga ccttaatcat attgcacaga tcacaagagt cattgccggg caatacaaaa
    51061 actctgtcgg accatggata gaatttttgc ccattcttaa atgatcattg tttgaatgaa
    51121 tcgggatttt ataaacttaa aaaataactg acattgatgg acgttgtgat caatcagtaa
    51181 tgaccggcat aaaaaagaat caggaaactt tttattaaac ttgaaatgaa tataaaataa
    51241 atatatttta tttttctttt aaaaatttaa acaaataaat tatgccaaca atcgtagttc
    51301 gtcacaaagt aggagatttt gatacctgga tcaaaggtca tgaggataga gtaaatgtat
    51361 ttgctcctgc agtaagcagt tttaaaacat ttaaggatgc agatgatcct aattctgtga
    51421 tattagtaat ggaagtcaat gatatggaaa aacttcaatc aattttaaat gatccttcta
    51481 caaaagattt aaaggacaag catactgtac ttgaacctat cactttatca atgcaggtag
    51541 atgcctgatg tttcagcatt tttgagtaaa caggattttt agttttcgtt cctgaaaatt
    51601 ttattagcag gtacattaga ttacatgcac aaggaaaact ataattacaa tttatgagaa
    51661 tgaaaatatt gctgcttttt atttgaaaaa attttttatt ttcagaggat tcttgatatt
    51721 cttcccgctt aggttcccaa gtccgtcata ggctgcatat ttatacagat catgaaatgt
    51781 aggataatta aatactgtgg ctatcatctg atcaagagtt ttcttatcat gtacaagtgt
    51841 catcccgtaa tgaataagtt cagttgcaat ccttcctatt atatgaactc cgaggataac
    51901 ttttgattca ctctcaaaaa ccagtttaag aaatccgttg tccggtgaac cctgaatttt
    51961 acctcgcgca atatcaccta cacggcttac acctgtatta tattttatcc catttgatag
    52021 agcttcctgt tcagtcattc ctaccatcga aacttcaggg acagtataaa ttccataagg
    52081 aaatatcttc tgaacaaaat ccagatcgtt ggttccaaac atatgagaaa ttgcaactct
    52141 tccctgatcc atacttgtac ttgccagagc agggaatccg atcacatctc ccactgcata
    52201 tatatgagga atgttggtta cataatattc atttacgata atcgcttccc tctcacccat
    52261 gataatgcct gctttgtcac agttcagtcc cttagtattt ccgcttcttc ctgctgcata
    52321 cagcaccatg tcggattcca gctcatcacc atttttaaga gtcatgtgaa tgctgtccga
    52381 tgcttttttt ggtttggaat attttgtaat acaggtatta aaaagtattt caattccatg
    52441 tatcttcatt tgtcttacaa gtgcatcagc aatatccgaa tccagaaacg gaaggatctt
    52501 gtctctgtta tttactaaaa atactttcac acccatagcc gcatagatag ttgcatactc
    52561 acagccgatg actcctgcac caatcacgca taatgatttc ggaaaacgtt tgatcttaag
    52621 tattgtatca gagtctaaaa ttcttttcct gtcaaaaggg atctcgggag gatttaccgg
    52681 atatgaacct gtcgctatga gaataaactt tccgtttatt tcaattacat tttcattttc
    52741 acattgaatc tgaatattgt gagcgtcaat gaatgaagca aatccggtat atacatctac
    52801 tttatgtctg ctgaaatttt tatcaacttc attctgcatg tcatttctta ctatatcacg
    52861 tctgtatgtg aactgtttat atttagtatg agcggaatgt ttttctgtcg attcgaaaat
    52921 atgcttttca taaactcttg aaaaatacag tgctgtttct ttcagggtct ttgaagggag
    52981 tgtccctgtc tgaacacctg caccgccggt ttcaggagat ttctctatca gcgcaacttt
    53041 ctttccgaag tatgcggctt ttacggcagc tttctctccg gcaggacctg agccgattac
    53101 gatcagatca taagttttaa tttttggcat ttcccctttc aatgaaaata aaaattttga
    53161 acaaattata gatatcagat tgaaagttca aagtaattag taaaatttta acttaaacgt
    53221 tacattccgt ttcagaattt ttgaaaaatt taggaaataa ataaagaccg gcttctccat
    53281 tataaaatcc aggggaattt aaatcgaact gtgtttaatt atcaaataat ataatttaag
    53341 accataaata atttcaatat gcttgagctg aaaaatgtga acaaggactt ttatgaaggc
    53401 agtgctaaac attctgtgtt gaacaatttt gaattatccg tacctgaagg agaaattatt
    53461 atccttctgg ggaaaagcgg atcgggtaaa tctactctcc tgaatattat cagcggaata
    53521 gatgtacccg acagcggatc tgtaaagatc gacggaactg acattacaaa actttctgaa
    53581 aaagacagaa ctcttacccg cagaaataag ataggtttca tctttcagtt ctttaattta
    53641 attcccacac ttacagtaaa agaaaatcta cagctgccgc ttgaattgaa taatgatgat
    53701 gatcaggaaa tgagaataaa ctccatatta acagaagttg gacttcttaa cagagcaaat
    53761 tcttatccgg ataagctctc aggcggcgag cagcaaagaa tagcaattgc acgagcgctt
    53821 attcataatc cggatattat acttgctgat gaaccgacag gaaatctcga ctatgaaaca
    53881 ggattgcaga tcgttgatct tctggacagg gtggtcaaga aaaaaggaaa gacaatgata
    53941 atggtcactc acagtaaaga tgtgatcgga cttgcagaca gaatttattc gctgaaagag
    54001 ggcaaagtta ttgaaacaaa agagggaaaa gtgggattag atattgagta acctgcttac
    54061 aagatcaagt ttaagatttt tactaagaca tccctggcag tttggtctat ctgttctggg
    54121 aattgcactg ggagttgcaa ttgttgtttc gatcgatata gcaaactaca gctcagcaac
    54181 cgcattcaac ctctctatga atgccgttgc gggaaaatca actcatcaga tcaccggtac
    54241 tgctgaagga attccagact cattgtatag attttttaaa aaagaaaaag ggataagaaa
    54301 tattgctcct gtcattgaat catacgtcag cattcctgac tcgaataaac gtgtattcaa
    54361 acttctggga gtggatattt ttgctgaacg tcctttccgt gattatcttt cacagacagg
    54421 aatctcactg aatggcgggc ttaaagattt tcttaccctt ccaaattctg taatactttc
    54481 taaggaaagt cttgataaat taaaaaaagt aatcggtgat accttaactg ttctcataaa
    54541 cggaaagcaa aaagtacttt acattactgg aattataaat gaagatgaac aaaacagatc
    54601 cgcactggaa aatcttttca tcacggacat tgcatcggca caggagctga caggaaagaa
    54661 tgataaaata gattatattg atctgatcat aaataatgtt caggaagagc aacagataaa
    54721 aagtctgctg ccggacggtt atcagctgca gaaatccggc tcacgttcgg aaacagcaga
    54781 gcaaatgctt gaagctttca atatcaacct cacatctctg agcttacttg ccctgatagt
    54841 cggattattt ctaatctata atacaatgac ctattcagta gtacagagaa gagtattgat
    54901 cggaactttg cgttctattg gtgtaacttc aaaagagatc tcgggaatga tattgcgtga
    54961 agctctgata atggggatca tcggaacagt tacaggattt attgctgcgt atttcatttc
    55021 aaaaattctg ataatattta tttctcaaac gatcaacgat ctgtattatg tagtatccgt
    55081 aagggagatc tatatttctc cgatgatcat tttaaaagga gcaggtcttg ggataattgc
    55141 aactttactt tctgcaattt atccggccag agaagcatcc agagtccacc cgagatctgc
    55201 aatgatcaga tccgaacagg agtcatctct tctgagaaat gttccacgga tgagcattac
    55261 gggagtaata tttatgatag caggtgctgt aattcttctt ataccttctc ataatatctg
    55321 gcttagttat tttggaatat tacccgtcat cataggattt gccttaatgg ctcctttgat
    55381 aataataatg gttgaaaaaa ttttttcacc cttatataaa aaactgttcg gcattaccgg
    55441 aaaaatggct tcaagatctg tcctgcagaa tatcagcaga acatatattg caattgcagc
    55501 gcttgcgctt gcagttgcag caactgtcgg tgtcggtatt atgatcggca gtttcagatc
    55561 cactgttata aattggcttg aggcgagact gaatgcagat atttttattt ccgccccaag
    55621 tctcatttcc agaaggaatg atgctgtatt acccgaggaa attctctcaa agataaaagc
    55681 tcttgatgaa gtaagtgatt ataattttta ccgtgaactg atcttaccgc aggacggaaa
    55741 agaatatcat ataattgctt cgggaatcag taaaagaagc tattcaggtt ttcagtttaa
    55801 agaaggtgat ccaaatgaag tatgggagaa atttgagaac ggtgaagtgt tcctgtctga
    55861 accgtttgct tacaaaaatg acattggttt gggagaaaat attaaactga aaactgataa
    55921 cggcttgcag gattttaagg tagcagcgat ctattatgat tatgcttctg atcagggact
    55981 gattacaatg aattatgatc agttcagaaa atactggaac gctgaaggaa tctcaggtat
    56041 ctcagttttt gtaaaagaag gaaatacaat cgattctgta aaagataaaa ttcagaccct
    56101 cggaacagac ggtcagcagc ttgtggtaag aacttacaaa tatcttcttg attcatctgt
    56161 ggaaatattc gaccgtacat ttctcgttgc aaaggtcctg caaattctgt ctgtgatcgt
    56221 tgcctttatt ggaattctga gttcgctgat gtcactgcag cttgaacgca ggagagaact
    56281 cggaatactg agagcaaacg ggctgctacc ttcacagttg tttaaaatag ttatgttgca
    56341 gactttctta atgggattca cagcaggagt aatagcgctt cccctcggaa atgtacttgc
    56401 tgcaatactt gtatatatta taaacaaaag atcattcgga tggacaatgc agttcgaggt
    56461 tatgccttcg atactaatag aggctttagt actttctatt gcagctgcct tgctggcggg
    56521 tatctacccg ggatataaaa tgtcgaaaac ttcaccttca aacgctttaa aagaagagta
    56581 aaaatgagaa aatatttatt tgtaatatta atagtatttg cagtctcagg atgcaaagat
    56641 gagaaaacag aatccggcaa cggcggaatt tctttatcaa aagctatgag tgatactgag
    56701 acaggagata tgggttttga aaaagctgtt gaaaaaagaa agtttatttt tcccgatgat
    56761 cacggtcccc atcctgaatt cagaacagag tggtggtact tcaccggcaa tcttacatct
    56821 tctgacaata aacaatttgg ttatcagttc acgatattca gaacagcatt aaatacagat
    56881 acaaataaag gaatttccga atggagttca aaccagatat atatggcaca ttttgcagta
    56941 actgatattt caggaggaaa gttttttttt gatgaacgct tcagccgtga cggcaacgaa
    57001 cttgcagggg cacaggcatc acctttcaaa gtctggcttg aggactggaa aataaatgaa
    57061 ataaatggag cagaaggcag aacttccttt gaactgccgg tacttaatat tatttcaaag
    57121 tcagataaat ctgagatcaa ttttattctt gaagcacaaa aaccattcat acttcaggga
    57181 gacagcggac taagtcagaa gggaaatgga atcggaaatg cttcatacta ttattcctat
    57241 acaagattaa gaacagaagg taagatcatt attgaaggaa aaaaatttga tgtaagcgga
    57301 aattcctgga tggaccgtga atggagtaca agcgcactga gcggagatca gaaaggctgg
    57361 aactggttcg cacttcagct tgacgacggt tcggagatta tgtattatca gatgagaaaa
    57421 aatgacggta cttctgatga attcagcaaa ggaacggtca tcgaaaagga cggatcatct
    57481 caattaataa aaaatgaaga tgtgataaac gaagtgacag attactggga aagtccgtcg
    57541 ggagaaaaat atccttcagg ctggatactg aaaatccctt cacagaaaat tgaattaaaa
    57601 attgctcccg cattaaaaga tcagctgatg aatgtctcag taaaatactg ggaaggttcc
    57661 gtaaatattg aaggcagaaa agacgataaa gaaataaacg gaagaggata tgtcgagttg
    57721 accgggtatt agaaatggag tttgacagac agaaattctc tgatatcaat gacttgagtt
    57781 acggaattac aaatattttg taagcctttt caatataact ttccttttta tttacagaat
    57841 attttacacg aaaaaagata aatttcttaa ttgagtcata aacttaaatt tttaatactt
    57901 agtataattt ttaaaaaaat aaaaacctat tatgaaatta ccataccttc cctacgacaa
    57961 atggactgac accagaatta cacttcacct tatctttcag ataatcggaa agataagact
    58021 aaaaacaact ccgcgaaaaa atcactggtg gtatgtcact ttatatcttt cccctaaagg
    58081 attttcaact gatcagattc ccatgagtga cggcttaaac acatttgaaa ttatttttaa
    58141 tgtacataaa aaagctgtgc agctttacaa cagcaaaggt gaagaaaaag aaattccact
    58201 ggaaaacgga tatactgtaa gtgagtttta taagaatatc atgggaatat taaatgaatg
    58261 ggatttacat ccggagttta ttgacaaacc tatggatatg ataactgata aaccgtttga
    58321 taaaattact gaatttcatc attatgactg ggactcaata aatttattct ggcagatgat
    58381 gttatggaat gacggggttt tcaaagagtt cagcggaaga ttctatggca agacctgtcc
    58441 tgtgcatatg tactggcatc acatggacct tgccgttaca aggttttccg gaaaacctgc
    58501 accgccaatg ccgcatgatg ccagaatttc tgacaaagat gcatattcac atgaagtgat
    58561 aagttttgga ttctgggcag gcgacgataa tttacctgag ccggcatatt attcatatac
    58621 agcacctgcc cctaaagatc tggataagga atcaataaaa cctgaacagg cattctggat
    58681 ggatgcaaac ggaagtccga tggcaattct gaaatacaaa gatctcctga attttgatga
    58741 tccgcggaaa gttcttatgg aatttttgga aagtgcatat ctttcaggtg caaaacttgc
    58801 gggctgggat attgaagctt ttaaagtaac gcctttgaaa gatctttaat tttataaaaa
    58861 gtttttgaca atgcagacat tcaaaattct gacttcttac aaatcaattt acaaagaccc
    58921 tattgttgta atacccgggg agatagttaa aattcttgaa attgaaaaag aagaaaagtg
    58981 gaaaggatgg attaaagtgg aaagcacttc aaacaccggc tggattccca ttcagataat
    59041 tgaattttct gatgacggaa atacggggaa aattcttgaa aattatagtg caaaagaact
    59101 ggacgtcgga cagggtgact tgatacaaaa aatttattcc cttaacggct ggagctggtc
    59161 aaaaaaaatc attgataatg aagaaggctg gatccctgat gaaattatcg ggtagattct
    59221 gattgaatta acagacagat ctgtaataaa aacaatatat aaaaaatgaa aaaagtttta
    59281 gactggcata ctcaagaagt agttaatatc tttgatgaag caagtatatg ggccgcgatg
    59341 tttggcagac ttcttcttga aaatattcca atgaaatctg aagcgaaagt tttagatgtt
    59401 ggatttggac ttgggtttcc tttaatagaa ttgagtcaga gatttggtga aaattcaaaa
    59461 atatatggaa tagatatttg gcacgaagca attatcagag caaaggaaaa gatccgggta
    59521 cttgaattat caaacattga gatcttcgag caaagtgctg aaattattcc ccttcctgat
    59581 aacgaactgg atttaatatg ttctaatctg ggtgtaaata attttgaaaa cagggaaacc
    59641 gttctaaagg aatgttacag agtattaaag accggaggtt ctatttgcat ttccacaaat
    59701 ccggttggga catttaaaga attgtttgat ctctaccatg aattattaaa ggaaaaaaat
    59761 atggatgatt gtttgttgga tttcagaaaa attattgatc acagaggtac agaagaatct
    59821 ataatttcag aatttgaact gtcaggtttt aagtattcga aaaaatctac tgataaaact
    59881 tttattaaat ttacaaatgc aaaagcctta tttgatcatt cattaataag gattggtttt
    59941 cttgaaagct ggaaaactat aattccttca gataaacgaa atgaattttt tgatttatta
    60001 attaaaaaaa tagacggaaa cattttaaag aatgggaatt ttataatttc aattcctatg
    60061 ctttatctcg aatttaaaaa ataaaactaa attctgcata tatttagctt aattcatttg
    60121 aacagtttat tttatcaaaa tcattttcct gatctgaata aaatcaccag cctcgatttt
    60181 gtaaaaataa attccacttg ctaaaatgtc tgcactaaag ttaactccat aatatccggg
    60241 ctgtttatat tcattgataa gaactgcaac ttcacttcca aggatatcat aaacttttaa
    60301 agatataatt gatgcttttg gaatcgcata ttcaatagta gttgtggggt taaacggatt
    60361 aggataattc tgaaatattg caaatttctc aggtacttca tctgaatttg aatttatccc
    60421 tacacctccg ttttctgtat agaatatcct tccattctgt ccgactgtcc agcctttgtt
    60481 ttcatttaca aaaaaaacac tgtaaagatc tcctgtaaaa ggtatcgttt gcgaaaccca
    60541 attatctccg ccgtcagtgg tctttaggat cttacctgtt cccacagctg tacctgatcc
    60601 tgagtttgaa aaatagatgc tgaaaagatt atttgcagta tttgaatttt gcgaaaccca
    60661 gttatctccg ccgtttgttg tctttaaaat tttaccgtta tatcctgcca caaaacctgt
    60721 attctgatca gtaaaaaaaa tacttctgag ttctgaagtc aatccgctgc tttgacttgt
    60781 ccagtttaaa ccgccattat ttgtcctgag aattgttccg ttaaatccgg ctgcataagc
    60841 agtcgtactg ctgatcatat taactcctgc aatctccgaa ttaacaccgc tgttattcag
    60901 tgtccacgtg gctcctccgt ttgttgttgt tattattaca ccattttgtc ctcctataat
    60961 cccgttctgc acatctataa aattcacatg tacaagatgc tctgttgtat tgctgatctg
    61021 tgaaatccag ttttgtcctc cgttagtcgt ccttaagatc agacctgact caccgactat
    61081 tgtaccgttc atttgatctg aaaagaaaat gtctcttaaa aatcctgtcg ttccggaagg
    61141 taaaacagtc cagttatttc cgccgtttat tgtttgaagg atcagacctg atgttccgac
    61201 gatgaaaccg ttcatcggat ctggaaatga aacacccagt agtgtttcgt tagtattact
    61261 tgtctgttgg atccagtctg catttgcatt tgtaatacta aaaataaaaa tgagagttag
    61321 taaagtattt ttcatttgtt aattgatgtt taaattattt aaaaagtttg tttgatatat
    61381 attaaaaaac ttaatactat tactatctga tctatatatg aatgaatgct acaaaattaa
    61441 ttactatttc gaatcagggg atttatcctt atttaagcaa aaccattctt ttagtttcag
    61501 ctgattctcc cgcctgcagt ttataaaaaa atacaccgct ggggaaatta cttccatcga
    61561 aagtgacaga gtagctgccg gcagattgtg tttcatttac taaagtcgtt aattcatttc
    61621 ctaaaacatc atagactttc agcgaaacat cactgacact tgaacattgg taagtaataa
    61681 tagtcgtcgg attaaagggg tttggatagt tttgtttgag ttcaaaaaaa tctggattga
    61741 tactctgatc ttttacagaa gtaatatttc catcttcact tacagtaata tatctgttca
    61801 cctgcagatt agtaaaaaca gaagttgttc ctgaaagcca ttcaattttc attgaatcta
    61861 taacagcagc atttcctaag ccaaaattca ggatgagatt tccactgtta tatccggact
    61921 gagattgtat ttcccttaac tgccacaccg gcaatccgtt tatctgggct ttgatcctga
    61981 cttttgtccc aattgcgctt ttcgaggaaa tgacaccatt gcatttaatt ccgatccagt
    62041 tattgctgtt actattgttc acatataaaa aatttttctg attaagacga ttggctacaa
    62101 acaaatccaa cgcgccgtca ttgttgtaat caccgaacac acatccaaaa gaatttccac
    62161 cgtcatttac tacagaaccg gcggaaattt ttgtaaaatc ataatcagga gcaccgttat
    62221 ttaaatataa aaaattattt tgaagatcat cgttgcaaac gaagatgtcc agatcaccgt
    62281 cgttgtctat gtcaccccag cagcttccca cactgcttcc gccgtcattg gaaactatac
    62341 ctgtatttaa tgctgtcagt tcatataaag gtggaccgct gtttctgtaa agaatattat
    62401 tctggtcaag aaaatttgca gcaaatagat cctgatttcc gtcattgttg taatcacccc
    62461 agcttccacc cagggttgat ttcccatcag caattacagt tttgttaaaa gttagatttc
    62521 cgttattcct gaaaagattg tcgttctgat tgccggcatt tcctataaac agatccggaa
    62581 gtctgtcatt attaaagtca ctccatactg cattaataga gaatgcattt ccgtcaagaa
    62641 gaaatgaagc tgtatctatc tttgtaaaat caaaagcggg ctgaccatta ttgcgataaa
    62701 gaaaatcgtt tccctgaaaa tttactataa acagatcaag atcaccgtca cggtcgatat
    62761 caagccagtg tccgctgttg gaatttgctg aatcatttac aatactgcct gtcattattt
    62821 ttgtaaaaga agaatcacca ttaccagtat atagaaaatt cttaaaaaaa tttctgttgg
    62881 ttacaaaaag atctgacttg ccgtcgttat tgtaatctgc aaatgctcct cctatcgaac
    62941 ttccaccgtc agtaacaacc ggaccacttt gcactttaaa gaatcctccg tttctgttat
    63001 tgatgaacaa agagttattc tgacttgtaa gatttccatt tgctacaaac agatcaagcc
    63061 atccgtcatt gttcagatcc acccagaatc cacctccact ttctaacatt tcgtttacaa
    63121 caagattatc cggatcggtg atctttgtat atgtctgtga atttgttgat ccgctgaaaa
    63181 gtattccata taaaattaat ataatgacga aattgatttt ggataaattc ataataaatt
    63241 tttaaatttt ttaaatattt aacgatttaa gaattattta ttttaaatta agaagaataa
    63301 attcataaag acatctcata tatatgagat acggcaattt aaataactat taaaaactgt
    63361 cggaaaaata tagatttatt ttgcaaaaaa ttttttttgt atccattttt atatctgtta
    63421 aaaatattcc ggatcatatt cattaaaata aaactttgat tattttttgc tttaaaaatt
    63481 ataacatatt ttcaatcatc caaaaatgaa gtttatttac gcaaaggaat tgaatcagat
    63541 gtttgatgta actggtcatt tctataattt aaaactatcc ggctcagttt ttaaatgcag
    63601 aagtgtcctt gaaatcagaa ggaaaaattc cggcgaagag aatgtgcttc ccgatattat
    63661 tgtgataatg atgaatcccg gatcttcccg accaatcgat aagaatttta aaataaatac
    63721 tttttcaaaa aaagagtacc taagtataaa ggtaagagat gttgtgccaa cacgacctga
    63781 taatgcgcag tatcagatca tgagacttat gatattcaac aaatggagtt ttgtcagggt
    63841 gctgaatctt tctgatctga gaaatggaaa cagcggcaaa ttcaaaactg aatttataaa
    63901 tgcaatgaag cttgataaga ctaatcctca ttgtcttaca cataaagatc ggagtaaaga
    63961 actgattaat tttttgaaat caaaaaacgg aaagatcatt gctgcgtggg gaagtatttc
    64021 cgagttaaaa attcctgctg aaaatatttt aaaacttaat aaaaatatca tcggcatcat
    64081 aaatggtaac gttccaaatt accgttatgc aagtccatat ctgaaatctc aaaaaacaga
    64141 atggcttaaa gagattcaaa aaaaattgtg aaatattata agaatgtaaa tgtatcagca
    64201 tatagtaatc tcaaatttta ataaccttaa caattaaatt taaaaatacg gttggttttt
    64261 aaatccagca ctttcacatt gagtattaat ctgtttatcc ataagtttaa ataaatcaaa
    64321 aaaaattata taactaaatt ttaactatga aaactctaaa cgcatatctg atctttccgg
    64381 gaaactgcag agaggcaatg aacttttatc atgaatgttt aggcggggaa atggtaaaca
    64441 tgattacttt cgaaggcgca ccaatggaag tcgaagagaa atacaaaaac aatataatgc
    64501 atgcccagct taaatctgac ggcatagacc ttatggcatc tgacggaaag ccgggaggca
    64561 cacatatttc gggaaacaat atttctctga gtttaaatgt aacaagtgaa gctgaacagg
    64621 atgagatatt tgaaaaactt tctaaaggcg gaaaaattgt aatggaactt gaagacacat
    64681 tctggggagc acggtttgga atgattacag ataaattcgg gatccagtgg atgctgaatt
    64741 atcataaacc gcagaaataa ttttataaaa tttaatttaa agaccatgtc atattctttt
    64801 gataaaacac ttacagatat aagttttgac gatgcaatca ccaaagtcac agacgaactt
    64861 aaaaaagaag gcttcggcat tttaacagag atagacgtca aggaaactct gaaaaaaaag
    64921 attgatgccg atataaataa ttacaaaata ctcggagctt gtaatccggg ctttgcgcat
    64981 aaagctattc ttgctgaaaa taaaattggg gtgttccttc cctgtaatgt tatagttatg
    65041 gaaagtgaaa acggtgatat cgaaatctct gctgtagatc catctgcttc aatgtctgct
    65101 gttaataatg aaagtctggc aggaatagca gttgaagtaa gggataagct gaaaaaagtt
    65161 atagatcagg tttgaacagt atgaaatttt ttcaaataaa acttttactt ttttttctta
    65221 aatgtacttt caaaatattc acccgctata gtgaatccta aattctgata gcatttgatc
    65281 gccggtaaat tttccgaatg aacattcagt ccgatctgat ctgtagtttt aaacagatca
    65341 cggcataaaa cagaagtgag tttacggcaa actgatctgc ctctgtgctc agacagagtt
    65401 gtaatattcc caagagctgc aactttgaat tcgggagaat atacgtgaat acctgcaacg
    65461 cctataagtt catcttcaaa ataatatcca aagtattttc ccgtttctaa catcctcaaa
    65521 tcaaaccagt tatccgggta tgacttttta taaaattcac ttatcacatc aatgtcagaa
    65581 ggcgataacc ttttaatata atccaatcct ttattttcat tagcttcatt aaattcttta
    65641 aaatcatttt ctctcagaca cattttgaaa tgtctgccat aatccagttt atcatatgat
    65701 ctttcaaaaa cttcgtctaa tccttttgaa aagtgtgtgt aaatattatc aggcaggtcc
    65761 tttaaaattc tgttaagaag ttc
//
LOCUS       DAORVQ010000002        65493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_107_length_65493_cov_5.862374, whole genome shotgun sequence.
ACCESSION   DAORVQ010000002 DAORVQ010000000
VERSION     DAORVQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 65493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65493
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_65493_cov_5.862374"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..2139)
                     /locus_tag="PLX80_00305"
     CDS             complement(442..2139)
                     /locus_tag="PLX80_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY19264.1"
                     /translation="MKIQSKVNRLFMSVLMLFVLIPFLSFDSPENNSKIDLEVFSKNY
                     EGQLLKLLNNHKNPGKELPDWTQLDPEIDGYEGTRTKSFYKYLKTLSPEPVRSDVIVA
                     VIDSGFDIDHPDLNDNVWQNSAEVNGVSGIDDDENGYVDDFNGWNFLGNAKHLPYEFS
                     REYFRLKKLNTPVSDPYFIKVEEEFVEKQDEIMATYKGLNETLIEMEAAEDVLRKNNI
                     TTDPKKLQGISMSLPEGKTSDAASIILGVYLLFGMEKDDLAKVTDEYELKSKVLFDSI
                     DVSELIGDDPLKLDETNYGNNDVTEKDEEHGTHVSGVIASRKTGHAPYAKIMFLRAVP
                     NEGDERDKDVGNAIRYAVDNGASIINMSAGKYFSPYPEYVVDAVKYAEEKGVLFVISA
                     GNEGDDVKDIVNYPRKYTEENGEKKYFDNMIVVGASSWMKKWSKDKDPENKNAKFDLL
                     APFSNYSDEVVDVFAPGVQIYSTIPGGKYKSIDGTSMASPSVTGVAAVLKSYYPQITA
                     FQLKEAILSSVRKYDGLKVKVKDSNTKYDFSKLSKSGGVIDMLNAYKVAGELLRQTN"
     gene            2249..3238
                     /locus_tag="PLX80_00310"
     CDS             2249..3238
                     /locus_tag="PLX80_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQY19265.1"
                     /translation="MSDHYQIYLKFKKKIDDILFNMIKNEQPVTLYEPINYLLSSGGK
                     RIRPILVIFSCEAAGGDPDKSINAAVAVELLHNFTLVHDDIMDNADTRRNLQTIHKKW
                     NENVAILTGDHLIGLAYKYLFKTDTQRLKETADSFTEGIIEVCEGQSYDKEFEDRNDV
                     TLDEYIMMINKKTAKMLETSAAIGALTGNVDTEMLSNLKNFALNIGLAFQIQDDMLDI
                     MADENELGKKIGGDILERKKTFLLIKAIETISNETDKRLIMDICQNSELEITNEIIEK
                     VKEVYIKNGIIESAKAEIINYTLKADEYLNNIKDTNSRNRLKWFSGMLMERKF"
     gene            3296..3565
                     /locus_tag="PLX80_00315"
     CDS             3296..3565
                     /locus_tag="PLX80_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQY19266.1"
                     /translation="MLEKEVTILNKAGMHTRPASAIVRIAAKYKADFFISKDNYEVNG
                     KSIIGVMTLAAEQGSTLILRFEGEDEELLADEMLEFFEDGFGEVK"
     gene            3562..6933
                     /gene="mfd"
                     /locus_tag="PLX80_00320"
     CDS             3562..6933
                     /gene="mfd"
                     /locus_tag="PLX80_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQY19267.1"
                     /translation="MNEQIKEIRSLVFQSDLFKKISEIKKLNSEKFLFRGIHGSLISL
                     VNNYLYNNVSKRIIFITHDDDRLSKIKDDTEFLLESGNITSYTGRPNESDPVSNLLMN
                     LSGQDEFIIFLHISGLEDRTISKDKFLSSIIEIKKGEEFSFEKLTELMLEYKFEKKDF
                     VDAEGDFSVRGGIVDIFSENHDTPVRIEFFGDTVESIREFDLNSQRSVRDVDSIKIGM
                     NLSTEEDLEIPLTENITDYFPDDAMLIIDQPEITLSEIKDEKILSILDKHKQIHITAF
                     SGSDPHQSSENNSSAEININFGSKTQPDFHSNLKLLYQNLYENIKNGYKAFIMTSDER
                     QADRIKELIEDFEDESVMEQDDFIHHEDADSDRSKITVDKNFTIKNKFTVIPESFHYG
                     FIYQESKILIYTEHQIFGRYFKQIKKRKQKFKGLTFNELKELTYGDYIVHRDFGIGVY
                     SGLKKITVGNSHQEVAVLSYNGNDKLFLNLNSINLIKKYSGSEGHVPVLTRLGGGEWD
                     KLKARTKKQVMDIARDLILLYAKRKSETGHKFSSDTHWQKELEANFMYEDTPDQYSAT
                     VDTKTDMESENPMDRLVCGDVGFGKTEVAVRAAFKAVLENKQVAILVPTTILAVQHYN
                     TFRDRLASFAVEVDNITRLKPAKEQKKILAKLLEGKLNILIGTHRILSKDIAFKDLGL
                     LIIDEEQRFGVKAKEKLRSIKPNVDTLTLTATPIPRTLNFSLLGARDLSIINTPPKNR
                     KPIITEIIKLDWSNIAGLIRTEINRSGQVYFVNDKIKNLHQLGDTIKTYVPEAKIGIA
                     HGQMDGKELEDIVIRFIEKKLNVLLCTKIIESGLDIPNVNTIIINNADKFGLAELYQL
                     RGRVGRSDVQAFSYLIVPPEGKITKTAVKRLQAIEEFTDLGSGFNLAMRDMEIRGVGN
                     LLGKAQSGFVQDMGFDMYISIIEEAVLELKENEFKDLFKSEESLKKINESIEKKLEEK
                     HSLIENDINALIPKDYISNDTERLNIYRRLYEINDQSELEMISLELRDRFGDYLDDVE
                     NLLKIIDLKIAATKLGIEKVVIRDKTISIHFPQDKDNMIFESSFYSDLIERISNDRSR
                     KYTISPEKERLVIEVNTNNTDDKVRLTEAKNLLKG"
     gene            7015..8661
                     /locus_tag="PLX80_00325"
     CDS             7015..8661
                     /locus_tag="PLX80_00325"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HQY19268.1"
                     /translation="MGKTKINCPNCGVDIDVDEVFKQKAEEDIKKDYDEKLSRQVQQL
                     NIKKEEVDAEKLRVKRLIEEQDDIIRGKLIQERGKLVKEAEFAAKRELEFEMVKLKQD
                     VDIKSKENKQYKMKEVDLLNKERELKEKEEHMKIDMKKMMLEKEEEIREKAVKAEQER
                     NEIRMMEYDRELEEQKKQLSEMKKKEIEFKNKEKELKDSEEQLRIDLEKQMLDREKMI
                     EEKIQKREQEKSELKKMEFEKRLEDQNKELSEMKVREVEFKNKEKELNEKEEQLKLEM
                     EKQMADQLKMVEEKTIKKEQEKNELRQREYEKQLDDQKKMIDELKKKAEQGSMQLQGE
                     VQELAIEDFLKNTFPFDKIIEVKKGEKGADAVQLVINNLQQECGKIIYESKRTKNFSE
                     GWIEKLKDDQIKQQADIAVIVTETMPKDMDRFGQKQGIWICSFSEIKSLSIVFREMLL
                     RTHAVRSVNVNKGDKMEMLYSYLTSSEFRQQVETIVDGFSNMQTDLNREKIAMEKIWS
                     SREKQIRKVLLSTSSMFGSIKGLAGSSIEDIKSLEMPDGE"
     gene            complement(8717..9754)
                     /locus_tag="PLX80_00330"
     CDS             complement(8717..9754)
                     /locus_tag="PLX80_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19269.1"
                     /translation="MKYILTVLLLFTISSAELYAECLCKNTGLKVSSPKRLSDINDVN
                     FINELNDRSDVQETGCDLCGCFMGLDPNYSDNQIGVRYSSFNFFGADGELIVPESEPQ
                     TDHEIEPGVEYAEYYRNIELFGRFYITPKIRLFFNIPFSFNEIDKLQLNGMGDAKVFA
                     QYQLYNTDMVRETNFWQRFFMGAGVKIPTGVYNKQLVYGIIDPHFQPGTGAFDLLLSA
                     SHLMKFENPGIGFGTDIFYTVNGENKNGYRFANQFNVTFSGFYQFEATDFTFIPHAGI
                     YFETAERDTQDDVSVNSSGGTAFFGTGGLDLYYSNYSLNFTIQLPITQNLYGLQPQND
                     LRFFIGTSYSF"
     gene            complement(9764..10576)
                     /locus_tag="PLX80_00335"
     CDS             complement(9764..10576)
                     /locus_tag="PLX80_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19270.1"
                     /translation="MKIRIISSIFVIVLFATVYYSCSDDTVSVAPDPPVTVDLVKIDS
                     GYVPGASAIMSFYAEDSLKLGYNKIYIVLYDSATHFSITNSHVEFLPVNHGHSAPVEN
                     PGETAIDGKFPGAIVLTQAFPNGDLHWHFNFRVHNHQAQGEPEGEVEFEVPRILDNPD
                     RFKSIIMPDSTKLYVSYISPKTPVTGLNDFEFVIHKNEPELFPYDGSYTITSTPEFLS
                     TGHMSGNNVTPSHTSLGHYKGVVNLDQSGAWRLKIRLTKNTLSYDTFFDITN"
     gene            complement(10661..10984)
                     /locus_tag="PLX80_00340"
     CDS             complement(10661..10984)
                     /locus_tag="PLX80_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19271.1"
                     /translation="MFLHINFIGLYYSLYSINKKELVESVCEKKNDSCEAKCFLEKKI
                     NEENDSDAKSNTPVKEMKLKVSEFIVSGVTILNSPINISDQLINNSQKYSSGYPQLKF
                     DPPKS"
     gene            complement(11082..11492)
                     /gene="msrB"
                     /locus_tag="PLX80_00345"
     CDS             complement(11082..11492)
                     /gene="msrB"
                     /locus_tag="PLX80_00345"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQY19272.1"
                     /translation="MTEKINKSEEDWKKELTPEQYHVTREKGTERPFTGKYYTLDDDG
                     IYKCICCGNLLFDSKAKFDSGCGWPSYTEPLNEDSVIYEEDTSHGMVRTEVRCAKCDA
                     HLGHVFDDGPGPEGARFCINSVSIDFEKNADSEK"
     gene            11573..12319
                     /locus_tag="PLX80_00350"
     CDS             11573..12319
                     /locus_tag="PLX80_00350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19273.1"
                     /translation="MDREKKSITTDANQLSHNIYNDKEFAESYSNKIEYNSHNALYER
                     PAVVSLIGDPVGKKILDAGCGPGVLASFLADKGAFITAVDYSSEMIRLTNDRVENKAR
                     VLELNLNEPLDVFKDEEFDIIVSSLTIHYLKNLRLFLSECNRILKEDGCMVFSTHHPF
                     MDFSFHSEGNYFETELLTDEWPSYNIKMDFYRRSLSDIFNLIMESEFVIEELAEPMPV
                     SECKEKFPDAYETLTHKPWFIIFKIKKQQI"
     gene            12319..13125
                     /locus_tag="PLX80_00355"
     CDS             12319..13125
                     /locus_tag="PLX80_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQY19274.1"
                     /translation="MRAGYLQFRPKFGDVDFNIKKINSLIGKNKFDLLVLPELANSGY
                     LFSDKKELAELSEEIPNGKFCKALQEICSKNKCYIVSGICEVSGNKFYNSSILVYPDG
                     KIVTYRKIHLFNTEKKWFTPGDLPLRSNDITLKSSEKVKIGMMICFDWIFPETARTLA
                     LQGVQILCHPSNLVMQYCQRAMFTRALENHIFTITANRIGKDIKKDGEIFFTGQSVIV
                     SPKGEYIHTGSKNKEECLIVNIDPKIASDKFINPENNLYKDRRKKFYKIH"
     gene            13152..13844
                     /locus_tag="PLX80_00360"
     CDS             13152..13844
                     /locus_tag="PLX80_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY19275.1"
                     /translation="MRDLILLHGAIGSSVQLEKLADSLKDNFRITLLNFSGHGGKEIP
                     KDPFSIKLFAEDVLYLLDKGKFEKPDVFGYSMGGYVAIYIARYFPDKLNRIFTLAAKF
                     DWNPESSSKESKMLNPEKLMEKLPDFAKELSDRHMPADWKIVLQKTAEMMINLGNGNS
                     LSKEDLSKIKNVVRISVGDRDKMVSIEESMEAYRCLDNGSFLVMPDTPHPIEKVSVER
                     LSGEISEFFSDL"
     gene            13867..14133
                     /locus_tag="PLX80_00365"
     CDS             13867..14133
                     /locus_tag="PLX80_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19276.1"
                     /translation="MKTQSKDTSPEFEKILIEKLRKKSTAENLRTALSLSSLTIQLSK
                     RAILRANPGKSKRALDLIFVEAHYGKKHADKLEEYLNKIGDESK"
     gene            14499..14909
                     /locus_tag="PLX80_00370"
     CDS             14499..14909
                     /locus_tag="PLX80_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19277.1"
                     /translation="MIVYSENLKNITPEMLEGFFAGWKKYPDKNKHLEILKNSDFIVI
                     AMDDNKVIGFINAVSDKVLSAYIPLLEVLPEYQGRGIGTELVRRMKILLKDFYMIDVV
                     CDEEQQKFYKKFGFKKFSSMVIRNYENQKGIQNK"
     gene            14913..15452
                     /locus_tag="PLX80_00375"
     CDS             14913..15452
                     /locus_tag="PLX80_00375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQY19278.1"
                     /translation="MIIEIDSELHLELLNESHAPEIFELTERNRLYLDKWLPWVKYTK
                     SKEDTLMFIKNSTEQYKNKTGMQTVIKYKDKITGLIGLVEISYVRNVTEIGYWLGEEY
                     MGKGIMTMACKALTDHTFDNFNVKRINIICDVNNTASQNVPARVGYFNQGILKRDNFL
                     NGEYIDHYHFVMRRHEWEK"
     gene            15823..17511
                     /locus_tag="PLX80_00380"
     CDS             15823..17511
                     /locus_tag="PLX80_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQY19279.1"
                     /translation="MKNLIATFQSACLLACFSLINPAQSLAQLTYPTRYPTAVIPNNG
                     EGNRLFFLYMPIIYEDNVGQDTFKMYYVRVRKENNITKVHHYLARSTNYGITWTDTQS
                     VSVLSHETIIYNPDSSKHYLSFGKWTTGLNKKSYMVKSQSIDGGFNFTGSDSVMELGE
                     DKSFIWNEEEHEYWGYVRPYHIEPSCCNGEDPPTCVSFGTGVRKIALMKNDGYFGSSS
                     AWSARNVILEVDTTDYYLNSTSPDYRTQIYYMQVFRNGKDWWGLIGMYRVGDNGGENN
                     PHPSFANTLQYTVDVELVWSDNGVDWCRTNNRQPIIALHDSIKQIFAVGTVVQDSVYF
                     YSIESTLHHSQYYTTNYCSYPNDTIKNGKYFSIYLSKMSLDDLNEWRPSSNVNIYCAV
                     EGFLNTGTGKHNLRDTLTAELRNSTSPYGLAASIKSVIDSVTFLGNFNFPHVDPGNYY
                     LAIYGRNSLETWSDSAISVCNGIDAEHNFITSYFEAYGGNVVLKSGIYCIFSGDVNND
                     DAIDLTDLSLIDNDISNSVSGYVNSDLNGDGEVDIADATIADNNSNNSVSTIHP"
     gene            17547..19550
                     /locus_tag="PLX80_00385"
     CDS             17547..19550
                     /locus_tag="PLX80_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19280.1"
                     /translation="MKNFTKIILVTIFSLLTSSEIFSTPINQFINSVSPPLNANSVIK
                     SSDIVITFEQVMNGSTMINDNVKVFGYQTGLMNVSLDFNSVENTLTINPLNQFKNGEI
                     ISVTLTAGIKTISNQNISPHVFKFRAKALGGTGSFNLSSEISNTVDGYIRSGDIDRDG
                     DIDLVINNAVYMNNGNAFFNISESLNHQGRPDMFDADNDGDLDILISNNGTFYYYKND
                     GVGNFLLFSVNSGSIWGFGDLSGNGYIDIAYYINTRELMTKMNTNGIFNPGMSYNLPG
                     QCNINENYYDNLLFDDFNNDGGVDIISISGWVGGVSITGYDLCRKYNQLTNDGIGDFS
                     VQTVFNNYIEGAAPFIFDSFDSKSFDVNEDGLVDIASPYIEIRNNGNGSYTQIGDFGF
                     PFSNSLDFDANGNGFIDLCVNFNGALMCFNNNGTGVFTLTVAINNLNFSSGSASGDFD
                     NDGDIDIAFKEYNNDKVAILLNGDSPLPVELSSFTSSINQNSVKFNWTTSQEQNNSGF
                     EIERSDNSSNVQGVWKKIDFINGVGNSNTQNNYSFEDKNLTSGKYKYRLKQIDFNGSF
                     EYHELSTEVVIGIPSSTELMQNYPNPFNPVTNISYRLSENGFVTLRVFDNSGREVKTL
                     LNEFKEAGYFTTEFNGSDLASGIYFYKLTAGNFVQTKKLSLVK"
     gene            20574..22592
                     /locus_tag="PLX80_00390"
     CDS             20574..22592
                     /locus_tag="PLX80_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY19281.1"
                     /translation="MKQLIFTTYILMIGIISFNSGLSSPINPYINSVSPPMNANSVVK
                     TSNIVITFEQTMNGSTMTDANIKLFGYQTGLMTASLDYNSVTKTLTINPVNNFKNGEK
                     ISLTLTSGIKTIANESISPFVYSFKTKALGGTGIFTKSSGIPTIQVPYIISGDVDKDG
                     DLDLLISEKIYKNNGNAVFTFYSSISVSGYPVMADFDNDGDLDIQETSNNITYFFQNN
                     GNGDFIQTTSYPGLNGSIGDLNGDGYFDMVYFSNQNNTDVYIVKNSNGISSIDTTYQI
                     NHSCFNPQYVYSDRVMIDDLNNDGTLDLVAINGLGFAGPNIGFYSLCKTYHSFTNNGI
                     GRFSVQTFYSQILSDYSPEVLFVGGSKTFDYDNDGFNDMISPGLNLKNDGTGLHIDRG
                     YFNIYNNSLDADFNGDGYIDLINIFGAASYLIQYINNGDGSFTRNILFINSYSFVSSA
                     SGDFDNDGDIDIAEYENYGNEVAILLNGDSPLPVELTSFTSSVISNSVKLNWSTSNEQ
                     NNSGFEIQKSLVKNKTPEAWSKIGFEEGQGNSSSSADYSFEDKNLSSGKYKYRLKQID
                     FNGNFEFYDLSNEVVIGIPASTELMQNYPNPFNPVTNISYRLSENSFVTIKVFDNSGR
                     EVKTLLNEFKEAGYFTTEFNGSDLASGIYFYKLTAGNFVQTKKLSLVK"
     gene            complement(23157..24242)
                     /locus_tag="PLX80_00395"
     CDS             complement(23157..24242)
                     /locus_tag="PLX80_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY19282.1"
                     /translation="MSIKPYELKVSKKTFLNKEAVKIELEIPSSLKEIFEYKHGQFLS
                     VQLTIDGKEYRREYSLCSSPYSENTYEIASKNVKDGIVSNYFYNDLKVGDTLMSYPPQ
                     GKFFTELNPNNKKMYVMIAGGSGITPVISIIKSVLYAEPESHVILYYGNNSQDSIMFK
                     NELDELQNKHNDRFKLYYTVMESGTEWNGMKGFINTTDLDKILIDADNKVQIEKEFFI
                     CGPGEMMELVKKHLSGKNYSKEKIHIEYFDAPVSPDPVTVSEDTDEVSEEIIKDRNIK
                     VILNDSEYDVPVPEGSVILDAVIAQELDPPFSCRSGICTTCRAKLYSGKVKMDEREGL
                     SDSEIDEGYILTCQSHPLTDDVKLEYM"
     gene            complement(24315..24644)
                     /locus_tag="PLX80_00400"
     CDS             complement(24315..24644)
                     /locus_tag="PLX80_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HQY19283.1"
                     /translation="MKEKERLKIATIKEEVIKVLKTCYDPEIPVDVWELGLIYDIVPA
                     TNGDVLITMTLTSPSCPVAGILPGEIEQKVREHPMVNESKVVLTFDPPWGQSMMSEVA
                     KLELGFL"
     gene            complement(24862..25287)
                     /locus_tag="PLX80_00405"
     CDS             complement(24862..25287)
                     /locus_tag="PLX80_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HQY19284.1"
                     /translation="MNIKEREESIIEDFSLFEDWMMKYEYIIELGKNLPMIDEKFKNE
                     SYEVKGCQSKVWLNAEMKDGLLYFKADSDAVITKGIISLLIKVLSGNKPADIVNADMN
                     FINEIGLKEHLSPNRANGLTSMINYMKKFALTYSGQNVN"
     gene            complement(25296..26513)
                     /locus_tag="PLX80_00410"
     CDS             complement(25296..26513)
                     /locus_tag="PLX80_00410"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQY19285.1"
                     /translation="MNVINLRKEFPILDQTINGKPLIYFDNAATSQKPNSVINAVSEY
                     YETINSNVHRGIHTLSERATEAYENSREKIRRFINAESSGEIVFVKGTTEGINLVASS
                     FGNMIIEEGDEIIISQMEHHSNMVPWQNLCSQKKALLKYIPVNEKGELILDEFKEMLN
                     EKTKLISVVYISNSLGTINPVKEIIETAHSQNIPVILDAAQAAPHQKIDVKDLDCDFI
                     AFSGHKMYGPTGIGILYGKEKHLEKMPPYQYGGEMIKSVSYDKTEFNVLPYKFEAGTP
                     DISGAIGLGAAVDFIEDLGHENIHKAEMELLDHANKLFSDIGGIRIIGTAKNKASVIS
                     FVMDGIHPYDTGSILDNLGIAVRTGFHCTQPLIEERFKLPGTVRASFAVYNTLEEIDE
                     LAKGLEKVKTMLL"
     gene            complement(26547..27914)
                     /gene="sufD"
                     /locus_tag="PLX80_00415"
     CDS             complement(26547..27914)
                     /gene="sufD"
                     /locus_tag="PLX80_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQY19286.1"
                     /translation="MENNLKENIIDLKDSNVTDLKKKLINEFQKFESSLNGEASSDYH
                     QIRKDAIAVFEDLGFPVKRMEEWKYTNLKPLTDHDFHYTFENSSELKSSDIEKYLFAK
                     ENINLLVFVNGIYSSELSSVISNCENIFIGNIQEGKKQYTEIFEKHFSKYADFKNRSL
                     VALNTAFSGDGAFIYVKKNTELKEPVHILHISDSEKENVMSQPRNLFIIEEGSKAEIA
                     EDYYSKGDNYSFSNMVTEIFCGKNSMTGHYKIQNEDSKSYHVGMTQVHQEQDSNYSNT
                     TISWGGSIIRNDLNALFDGQNTESHFFGLYLLSGKQHVDNHTLADHKMPNCFSNELYK
                     GVIGGQATGVFNGKIMVREDAQKTNAYQSNGNILLSDDATIYSKPQLEIFADDVKCSH
                     GATTGQLNENEMFYIKSRGIGDNDAKKLLLKAFVSDVLDSIRIEEFRNLIEEKLEVKL
                     SEQLK"
     gene            complement(27942..28715)
                     /gene="sufC"
                     /locus_tag="PLX80_00420"
     CDS             complement(27942..28715)
                     /gene="sufC"
                     /locus_tag="PLX80_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQY19287.1"
                     /translation="MLTIKNLKAKIETSEGDKQILKGINLTVNPGEIHAIMGPNGSGK
                     STLASVLTGREGYDVTEGEVIYDGKDLLELNPEERAREGIFLAFQYPIEIPGVSNVNF
                     LKTAINEIRKSKGEEPMSSVDFLKMMKEKAKLVEIDPAMLSRYVNVGFSGGEKKRNEI
                     FQLAMLEPKLSILDETDSGLDIDALKIVANGVNQLKSENNAFIVITHYQRLLNYIVPD
                     FVHVISDGKIVKSGGKELALELEENGYEFINEIKENAEA"
     gene            complement(28788..29183)
                     /locus_tag="PLX80_00425"
     CDS             complement(28788..29183)
                     /locus_tag="PLX80_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HQY19288.1"
                     /translation="MNEKYYPSEKEEYLLKIIVDCAYQVHINLGPGLYERIYEVCFCY
                     ELRKRGIEFSSQVSVPIIYDELKFEKGFRIDVLVEDKIICELKAVHEHNPVFDAQILS
                     YLKLSKKHLGMLINFNVPLIKNGIKRFII"
     gene            complement(29259..30704)
                     /gene="sufB"
                     /locus_tag="PLX80_00430"
     CDS             complement(29259..30704)
                     /gene="sufB"
                     /locus_tag="PLX80_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQY19289.1"
                     /translation="MSNEAEILEKVTSSDYKYGFYTDLEMDKAPKGLNEDIIKFISNK
                     KNEPEFMLEWRLKAYKLWQEMKEPKWPNVKYPPIDYNDIIYYAAPNTKKKLQSLDELD
                     PEIKNTYDKLGIPLEEQLILAGVAVDAVMDSVSVITTYKEKLGELGIIFCSMSEAIEN
                     HSELVKKYLASVVPQTDNFYAALNSAVFTDGSFVYIPKGVRCPMELSTYFRINAENTG
                     QFERTLIIADEGSYVSYLEGCTAPMRDENQLHAAVVELVAMKDAEIKYSTVQNWYPGN
                     KEGVGGIYNFVTKRGICAGENSKISWTQVETGSSITWKYPSVILKGDNSIGEFYSVAL
                     TNNYQQADTGTKMMHIGKNTKSRIVSKGISAGKSQNSYRGLVQVSKRADNARNFSQCD
                     SLLMGDRCGAHTFPYFEIKNKSAHVEHEATTSKIGEDIMFYCNQRGISQEDAVALIVN
                     GYAKEVLNQLPMEFAVEAQKLLAISLEGSVG"
     gene            31209..32159
                     /locus_tag="PLX80_00435"
     CDS             31209..32159
                     /locus_tag="PLX80_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HQY19290.1"
                     /translation="MENKKIITVFGATGAQGGGLVRAILSDKNSEFTARAVTRDKNSD
                     KAKALADLGAEVVSADIDDAESIKKALEGAYGAFFVTFFWDHFSAEKEYQQVTDFVNA
                     AKDSDLKHIIWSTLEDTRILVPLEDDRMPTLQGKYKVPHFDGKGAADKLFTEAGLPVT
                     FLRTSFYWDNLIYFGMGPKKGEDGNYYIAFPMDDKKLPGMAAEDIGRCAYGIFKKDGE
                     VTGKTIGVTGEKLSGAEMAEKLSKSLGINVIFNNVTPEQFRNFGFPGADDLGNMFQFK
                     RDFNEEFNGIRDEVYSKELNPDLMDFDKWLSEYGSKIPLE"
     gene            32160..32549
                     /locus_tag="PLX80_00440"
     CDS             32160..32549
                     /locus_tag="PLX80_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19291.1"
                     /translation="MFIVSKYIVILFGIFLITAGILMFIAPKKVQQIIGKAGSTNFIN
                     YAEITIRMIPAAALVIYSDLSRYPDLFKVFGWFMIITSFVLYLIPPKMHHAFSVKSSG
                     KLKPLYLRIISPFSILIGIFLIYCVSI"
     gene            complement(32594..32752)
                     /locus_tag="PLX80_00445"
     CDS             complement(32594..32752)
                     /locus_tag="PLX80_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19292.1"
                     /translation="MNLKLKNKTIREAANTSILAAVASILSSFDFANSFNSAAGVAFL
                     PLTFLMVA"
     gene            complement(32730..33392)
                     /locus_tag="PLX80_00450"
     CDS             complement(32730..33392)
                     /locus_tag="PLX80_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19293.1"
                     /translation="MNDIEIINLWKSNSKKINENLLLNKKNAEDLIKMKTQSLLSSMK
                     PVKIFTVMMAVLWVGIGGTVVTNIFLSAYSSVNHFFLYSAAIQILLTAIALVIYLYQT
                     ITIYQTDITEPILATQEKIAKLISTSLWVTRFMFLQLPLWTIFYWNKNMLENGSLPLL
                     IIQGIVTLSFTIAALWLFFNIKYENRNKKWFRIIFNGKEWAPLLNSMDLLSQVDEFKT
                     EK"
     gene            complement(33402..33923)
                     /locus_tag="PLX80_00455"
     CDS             complement(33402..33923)
                     /locus_tag="PLX80_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY19294.1"
                     /translation="MKSTQDKNAIFVSVIESHKGILYKIANAYCKDSEDRKDLIQEIY
                     IQLWESFDSYNDDYKYSTWIYRISLNVAISFYRKENSRSKISSPFNTEIFNIADTINI
                     EKEEENINLGILQKFISELKELDKALILLYLEEKSYKEIADIIGFSETNVATKIGRIK
                     LKLKEKFSQIINQ"
     gene            34391..37675
                     /locus_tag="PLX80_00460"
     CDS             34391..37675
                     /locus_tag="PLX80_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19295.1"
                     /translation="MEFKPTGSVTFLFTDIEGSTKLSQEFPDSLQASLDIHHRILHEA
                     IESNNGFVFEIIGDAFCAAFENAGDAVKAAVDAQLYLAYEKWFNAVIKARMGIHSGIA
                     EWNGKRYLGYITLARTARVMSAAYGEQIIVSNDTFESLFNNCHKDSKTLIFLDPKTSS
                     LRDFEVEERKEISFRDLGERRLKDVIKPIKLFQVTAPGLREDFPPLKTLDARLNNLPV
                     QLTNFIGRENELKQIKDSLSNTHLLTITGSGGSGKSRLSLQTAADLIDIFANGVWFVE
                     LANLNDPVLLPVSIMSVIGILENPKESPEVTLKGYLKDKEILIILDNCEHLINACAVL
                     VESLLSLSPKLKVLATSREPLKCSGEHLFRIPSLSTPVQGSKETPEQLTQYESVRLFI
                     ERSLTLDQGFKVTNENASALAEICFRLDGIPLAIELAAARTKILSLEKIFERLDDRFS
                     LLTGGKRTALPRQQTLRSLIDWSYDLLSDNEKIIWNRLSVFRGGWELEAAEEICSDDI
                     IDKNEIIELLQNLSEKSIIIFDKELVRYRMLETIRQYGDEKLRTSEDYKEISKKHLKY
                     YTEHAETADLKLHGLEIVTWLKVLDVEKGNIEKVFMFSDESGDIITEARLAGALGYYW
                     DIRGQISEGILRLENIYLNIPETKNLGFIKIIFLIGLLTHRKGEFVKASEYLKESHDH
                     YIKLNYKPGIGYALNSLGDIFRDKGEYDKAADMYKESLEIRRNIGDKSGIADSLNCLG
                     NVSFDKGEYNEADELYKKSLEIRRNIGDKRGTANSLHWLGNVSNYKREYEKATGYYSE
                     SLSIRREIGDKRGIASSLNNLGNVYIIKKEYENATDLYEESLVTFREIGDKKGIADSL
                     NGLGNVSFDNIEYQKAADLYQESLAIKLLMGEKKGIAYSLNNLGNVFYHKGEYDKAAG
                     YYEESLGIKREIGDKRGITNSLNGLGNVFFYKGEYDKATSYYEESLEISREIDYEMAI
                     EDSLKNLGMVSIKRGEFNNAQKYYQEILSHSIDSGNKKKITENLLRLIDLNLKKKSDV
                     SFNKLLGFLNHYSNSNKIIFEKSDQKVFDDAVTYLKENMREEEFLKSFEEGEMMTLDS
                     ILNIKL"
     gene            37750..40590
                     /locus_tag="PLX80_00465"
     CDS             37750..40590
                     /locus_tag="PLX80_00465"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19296.1"
                     /translation="MTIPTGNITFLFTDIEGSTKLSQKFPDTMPAAMEIHNNILLKSI
                     ESNNGFVFKNTGDGFCAAFQNAGDSVRAAVEIQTNLLNENWKDAVIKIRIGIHSGKAE
                     WNGKNYMGYITLARTSRIMSSAFGDQIIISNDTYNLFSESNTLLQSHENIRSEKNNLI
                     SLHQSGDTKISFWDLGERRLKDLINPIKLFQITAPGLRENFPPLETLDARPNNLPVQL
                     TSFIGREKDIKEIKKLTVDNRLVSIIGPGGIGKTRLSLQVAAELIDQYSDGVWLTEFA
                     QISDPELISQTTAKFLGINEQSNQETIITLINYLKDKELLLIFDNCEHLVSACAVLTE
                     KLLQNCAKLKIIVTSREAFRIEGEHLYNIISLSHPEPNEVNTPIELSQFEAVRLFIER
                     ALSVSPGFKIDNENAPALAQICYHLDGIPFAIELAAARTKILTLEKICEKLNNRFQLL
                     TGGKRTSLPRQQTLRAMIDWSYDLLSHKEKLLLQRLSVFSGGWTIETAEQICADEEID
                     DSEILDLNSLLLDKSLISAKESSGSIRFFMLETIKEYTKEKLEKMNDVYRKHFSYFNQ
                     LCNCDEMRSKGLTQLQWIKLVDTELDNIRAAIQWAVEKNSDEACDIISTTGIFNNIKG
                     YYKEGLQTCLKILNSSLSVNELPKVKVLYTAALMASNLGNSADAEYYAKEGLRVAKKY
                     NDKHKIASCLNVLGSSINKNLERSAEALEYFEEALSIFREVNNKTEIGVLLFNMSTTV
                     NIKGDPKLVLKYRLEAMKIFKELDDTHNISLILASLGVYELRENNFELAKNYTEESLA
                     IAHEIGDNFLISVNLVNLGCIYSGLNQYEKAAELLERSIVIIKEHGYKESYLVAVMYL
                     GEVFSNSGDYKRAIELFNESIQSGTDSNNDYFLAYNYFNLGVSYFKLKDYVNSLKYFY
                     LLKSDKENSKLNISNEKLKIAEEYISKLESIE"
     gene            41077..43878
                     /locus_tag="PLX80_00470"
     CDS             41077..43878
                     /locus_tag="PLX80_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY19297.1"
                     /translation="MNNKPNGSVTFLFTDIEGSTKLSQEFPGTLPAALEVHNKILRDA
                     IETNNGFVFKNTGDGFCAAFEDAFAAVKAAVDAQSGLANEKWSDAVIKARMGIDSGNA
                     EWNGKNYIGYKSLSRAEKVMSTAYGEQIIISGDSYELVKKNFESSDKKIFNKAGNEIT
                     FTDLGERRLKDENNTVKLFQIATSGLKEDFPLLKTLDARPNNLPVQLTSFIGKEKEVK
                     IIKKLIAENRLVTLFGPGGTGKTRLSLQVAYGLIDHFTNGVWLIELAPISDPDLIAQT
                     IANSLKINDQSDQNTIETLNNYLKDKELLLIIDNCEHLVTACAAFTEKLLQNSKNLKI
                     IATSREAFRSKGEHTYYVTSLSHPELNEVVSLNELSQFEAVKLFISRALEVNPGFSIN
                     NENAPALAQICYQLGGIPFAIELAAARTKVLPVEKICEKLVDRFKLLTGGRRTDLPGH
                     QTLKAMIDWSYDLLSEKEKILLQRISVFSGGWTLEAAEKICEDDEIEDLEILDLNSHL
                     LDKSLISAKESSGEIRFFMLESIKEYAREKLGEKNEVFKRHFDFFKELSVYETFRSKG
                     VTQLQWIKLIDNEIDNLRAAIKWATLNYPDEACNIISSSGEYYKIKGYYLEGHQNCIK
                     LINSSLPITDKSKVKLFYTAGLMTESLGNETDAESYGKEGLAIARKYNNKLEIVMCLN
                     LLGVSTNKNLARREEAMKYFQEALTLAREIDAKGEIGSILLNMSPILNNKENQVMNNK
                     KEALKIFKELGDTRNVSLILASIGVYEFKQENLESALVYTEESLDIANLIGDKFLISV
                     NLVNLGCINLGLKQYDKAAELLEQSIVIIKDNGYKASYLAAVMYLGEVLSKSGDDERA
                     IGLFKESIQTGTDMENNYYLSNNYFNLGVSYFNLKDYENSLKHFDLLKICLDKTNSVH
                     SNEKVKIAEEYINKIKNMM"
     gene            44037..44708
                     /locus_tag="PLX80_00475"
     CDS             44037..44708
                     /locus_tag="PLX80_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19298.1"
                     /translation="MKKIYSLKFLIVFIMFYSCGNSSEDVKVNSDVKGKSEVKGEIEL
                     KDKSEVKDLLGIKGPLEFNSTTFDLAWSDKPRENYYIQEYLPSGESPESFNQMMSVHL
                     FLTDLTVDKMVSKKIGELEDRKKSDPLCNYSSFKSPDGNEIIVDFVLSEDKEGQIPII
                     EFNAYRYKISDTGNGSKGIIIYAYSKRSYGDDIDSFLVNLKEDRMKYLDEMSKTTIPA
                     VSISK"
     gene            44957..46333
                     /locus_tag="PLX80_00480"
     CDS             44957..46333
                     /locus_tag="PLX80_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY19299.1"
                     /translation="MNKFLLILAVLSIYLNTSFGQLPNHNITLLANLNQHITNDTYSG
                     CWGYIAPDGSEYGILGTHLGTAFINISDSSNIHEVDFEPAPVTGSGNHWREITVHSHY
                     AYVITEQSNSGIEIFDLQYLPDSVHRVNKFIPPGHITSHTIARSGPYLFLNGCNSAFG
                     QGVTILDLTSDPENPVVRGSLNTHYVHDCRVVNDTIWAACLSSGIRIINAVNKDQPVF
                     INSFLTPPHLATHNSALTKDGNYLYTTDETSSPTAGTLKIFDVQDLGNITFIRDWRPT
                     GITTSQVHNIEIFGDTAVIAHRSSGIRVLDISNPIDPIEIAWYDTYPINNVNAFIGAW
                     GIFMFPSKKIIASNKEDGLWVVQLQNTTDIKLNSTVVSEYSLSQNFPNPFNPKTVISY
                     ELPVTGNALLKVFDVLGNEVAELVNEKQNAGSYSVEFDGSNFSSGVYFYKLTAGEFVE
                     TKRMILLK"
     gene            complement(46388..47614)
                     /locus_tag="PLX80_00485"
     CDS             complement(46388..47614)
                     /locus_tag="PLX80_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQY19300.1"
                     /translation="MRKNLFTLLLILFIPFINLYSQTYEDFTYVNSEVGYKYIGSYDV
                     NKLNEIFTTEAESFSDFEVTFSSVANGVKLYRVIYNSEIPEMNNKPTVASGLIAIPDI
                     ENKKMPLVSYQHGTVFTKTDVPSFPDESMETRLMIAQFAGNGYIVIGADYFGKGLSSE
                     QDSYQVKASTQQACLDMLKASDEILNSMNIEHGPLFLSGWSMGSWATLLFLQKLENLN
                     IEVTSTSVACTPTDVYAMINRWIHAPEKGDAVWLPGILALQLSSYSIYYDLPGLMESG
                     IKAEYQQATRDFYENKITFEEFYSKTPDNVDDYLKSEFKESSSSGENRYWQIIQNNNS
                     YRWRSRTPLNLYFGESDEAVPVYIGTLPVDYQLLMGGGKTISVSAGKLADHRGTFKYS
                     VEHSKKVYDEILKKNN"
     gene            complement(47729..48886)
                     /locus_tag="PLX80_00490"
     CDS             complement(47729..48886)
                     /locus_tag="PLX80_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY19301.1"
                     /translation="MVILKSSLTVILISLTIIVYLITPAVLNGKELKMPEDEDVDSSG
                     FSSDGPVIVYQDSKVLNYSVIPNMNETDGDLFEISVGEISKDDTLTCIVDETVNQFDF
                     KLKENIQIEKDTYELPEKMLIISDIEGNFRGFQMILEGAGVINENFEWTFGNGHLVLN
                     GDFFDRGINVNECLWLIYKLESEAEMHGGKVHFILGNHEIMVLQNDLRYVRQKYFDNA
                     DSLGLDYSKWYSPETELGRWLRSKNAIENIGGYLFVHAGLSKDVPYGKYTLTQINDSI
                     RYSIDIDFEREERRKNIFIGSKSPIWYRGIMEEEETQAEVDSTLEIFNANKMVLGHTV
                     VDSIKYLYNKKVIGIDLHHQENSDKGFMYALFFENGEFYVINNSGNKSLLK"
     gene            complement(48906..49949)
                     /locus_tag="PLX80_00495"
     CDS             complement(48906..49949)
                     /locus_tag="PLX80_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY19302.1"
                     /translation="MSQFVLMMFITGLLLYICWLMLAPFISILLWSAILAIIFYPVYK
                     KLFLKTNNHSISAIITIFVSLFTFVIPVVAVLASAINEISGISRTSLDEIKLILSDLQ
                     KGKFSFIYNYLNQFIRIEEFLKPEDIKGFINNIKEAFLSASLYFIEGAFGMIVGITLS
                     VFSMFYLFRDGEKIVKNIPEILPMESSQSKELIRETSALINATIRGTLFIAVLQGVLA
                     GLIFWILGIPSYILLGVLAMIFAVIPTGGTAFVTVPVIIVLLFSGEYAKAAVISVYAA
                     LVIGMIDNFLLPKLIKQRVKMNELFVFFSVLGGIQLFGLLGLFMGPIILAIALGFFKV
                     FRGEKIDKEEVSM"
     gene            complement(50129..50701)
                     /locus_tag="PLX80_00500"
     CDS             complement(50129..50701)
                     /locus_tag="PLX80_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19303.1"
                     /translation="MFRYYLDKEEEKLFGYNISFLIKLAAKSDPYQVKHGIIKTIPME
                     DSFFLHKGTDKRNMMIIFSKDNDFNKYIRKSYMGNYYFGFRISYINFIKTFVAKDAGK
                     RSLTVNKYLYSYIIKNKFQNKWLRNKNHMYIETRFKDHRKTHDFLILLDIQRRKNILK
                     NLEAFFKFRIQNENDQHELIKFFMKLRREM"
     gene            complement(50826..51515)
                     /locus_tag="PLX80_00505"
     CDS             complement(50826..51515)
                     /locus_tag="PLX80_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HQY19304.1"
                     /translation="MTNFADRVITFNSRLKISSALPEGVNSMNPFKEKSAVESSTQFY
                     KKFYNDNCKRFFVAGINPGRNGAGVTGIPFTDTKRLKDNCGIRFNGSETYEPSSVFVY
                     EVIDTFGGPEKFYSKFYINSICPLGFTIRNEKGKFVNYNYYDDPALTKSVYPFILNSL
                     EKQIDLGLETDICFCLGTGKNFKFMKLINDQHNFFRKVIPLEHPRFVMQYRSKQKPIF
                     IKKYMNSFAEL"
     gene            complement(51644..52135)
                     /locus_tag="PLX80_00510"
     CDS             complement(51644..52135)
                     /locus_tag="PLX80_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19305.1"
                     /translation="METLLKEVMWKQFGASIDMFENAVRACPEDLWDADSKYWYIVYH
                     TLFFLDYYLTTDPDNFSPPPPFTLSEFNPEGEMPERVYTKEEMLIYLMHCRNKCHDLI
                     AGMNPDNVLHRWKNEYRDYSIPEILLYNMRHVQHHAAQLNLLLRQNEADVPNWVSRTK
                     LNL"
     gene            complement(52196..53032)
                     /locus_tag="PLX80_00515"
     CDS             complement(52196..53032)
                     /locus_tag="PLX80_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19306.1"
                     /translation="MKNQIYPCLWFDGLGKEASEFYCNLFKDSKITAEFGPVVTFELS
                     GEKFMTLDGGPKFKINPSISFFILCESVEEIDLHWEKLSDDGKVFMPLDKYDWSERYG
                     WIQDKYGVSWQLMLGKYEDVGQKFTPSMLFSGDQLGKADEAIHFYTSVFKDSSITGIM
                     KYSEGEDQKEGLVKHAQFSLNGKVFMMMESEGHEFSMNEAVSIVVSCKDQKEIDYYWD
                     KLTDGGEESMCGWLKDKYGVSWQIVPDNIAELLSDPEKAGRVMQEVLKMRKLDMETML
                     NA"
     gene            complement(53123..53695)
                     /locus_tag="PLX80_00520"
     CDS             complement(53123..53695)
                     /locus_tag="PLX80_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY19307.1"
                     /translation="MRKLKLQTQITVDGFTAGPNGELDWMTWDMDEKLSQYINSITDT
                     SDTILLGRKMTEGFITHWTNVRKLPDDPGYEFAHKMMDKPKVVFTKTLEKSEWENTEL
                     AKGNLSDEINSLKKQDGGDIIVYGGAGFVASLISERLIDEFHLFVNPAAIGSGMSIFK
                     GLDSTQKLNLKNCTPYECGIAVLVYEKKDI"
     gene            complement(53838..54359)
                     /locus_tag="PLX80_00525"
     CDS             complement(53838..54359)
                     /locus_tag="PLX80_00525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016091955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19308.1"
                     /translation="MKTLKLISPSINLENEYKEMLEEWHDSGEELVPFILRYDPKNFK
                     KFVEQMEGFKNGIDIPETFVPHSTFWLVNSEDKILGVVNIRHKLNDKLSLIGGHIGYG
                     VRPSQRRKGYATKILELALEESKKLGIKKALVTCNKNNIASKKTILKNNGIFYQEKEV
                     EGVSVLSFWIDIN"
     gene            complement(54416..54967)
                     /locus_tag="PLX80_00530"
     CDS             complement(54416..54967)
                     /locus_tag="PLX80_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HQY19309.1"
                     /translation="MTTYISILRGINVSGHRIIKMDVLREMFEQLKFKNVKSYIQSGN
                     IIFKFKNTEPNKLSAKISDSINETFGFDVPVIVLELKDVERIIKSNPFSKETEIDPAY
                     LHVTFLSDIPDNNHVKKLSEIDFKKDKFYHKDNAVYLYCPDKYSNSKLTNGFIENKLK
                     VTATTRNWKTVNELLKIAQSIED"
     gene            complement(55110..55568)
                     /locus_tag="PLX80_00535"
     CDS             complement(55110..55568)
                     /locus_tag="PLX80_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HQY19310.1"
                     /translation="MPLPNIFTKEVSDKIIQRINNLSSETPPLWGKMNASQMLAHCTV
                     VYEMVYETKHKKPNAFMSFILKLIVKDKIVGEKPYSHNLRTAPQFIMTDSKNFDTEKK
                     RLIDFINKTQELGEGHFDNKENLSFGNLNKTEWNNMFYKHLDHHLSQFGV"
     gene            55768..56097
                     /locus_tag="PLX80_00540"
     CDS             55768..56097
                     /locus_tag="PLX80_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY19311.1"
                     /translation="MPKIKFNLDSGKVLRAAEILKTIGHPVRLQILEALQEKEPLSVS
                     EIQEKIGSNTEQSLLSHHLIKMKDRGILRCEKKGMNVLYRLEDRAILKIFECMESCSL
                     FYRKEYV"
     gene            56097..56768
                     /locus_tag="PLX80_00545"
     CDS             56097..56768
                     /locus_tag="PLX80_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY19312.1"
                     /translation="MNRINEKVITKHRSNNDGNVTCEDPEAIRKISNVESWNSNYRTG
                     NIGWDIGEVSPPIKNYIDQIKNKNIKILIPGCGNAYEAEYLSKNAFKDITLLDISSVV
                     TNELIKKFAKDKNINVICMNFFEQENKYDLILEQTFFCALNPDLREEYCKKMIRLLNT
                     SGKLVGVLFDREFDKDGPPYGGSKKEYEELLKKYFDVKVLEKCYNSVQPRSGSEVFIN
                     LKNKN"
     gene            56768..57340
                     /locus_tag="PLX80_00550"
     CDS             56768..57340
                     /locus_tag="PLX80_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQY19313.1"
                     /translation="MLELIKQPWHWAVSGTVIGLIVPMLLVLGNKKFGISSSFRHICA
                     ACFSGDIKFFKYDWKKEAWNLFFVGGIIIGSYIASTFLSDPADIIQVAPGTLETLKGY
                     GIKDFTSLMPLDLFSWDNLFSMKGLIFFVLGGFLVGFGTRYAGGCTSGHAIMGLANLQ
                     MPSLIATMFFMVGGFFSTNILIPLIFKLIQ"
     gene            57340..57855
                     /locus_tag="PLX80_00555"
     CDS             57340..57855
                     /locus_tag="PLX80_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQY19314.1"
                     /translation="MSNRNSDVQIETADEFEIRHQDVSCTNESKLVHPWWYNLKYTLV
                     GILFGIVFVKAEIISWFRIQEMFQLTSFFMYGVIGTAVVVGALSVFIIKKMKLKSIYG
                     EEISIDDKKFNKGQIYGAAIFGIGWAITGACPGPLFAQVGSGFSVVIVTVLSAIAGTW
                     IYGKFREKLPH"
     gene            complement(58247..59326)
                     /locus_tag="PLX80_00560"
     CDS             complement(58247..59326)
                     /locus_tag="PLX80_00560"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQY19315.1"
                     /translation="MKNTLLKSALVIFTMTLFIISFNSCTDDLTSINSGNPPALGGYE
                     ENEAGEIMTLAALCYTAENNPNHAEIKDSLILQLANTNYTTGGNWKLVWGPGISPDGG
                     NMIYVAADTTGDSSLYTIAIRGTDWIFPSNIKEDMEVWKLDRYPFTAGNLDSVAVGSL
                     DGLNTLLAITDNGKNLAAFLNGIQGSNLKMFITGHSLGGALATMLSAWFVDNGYTNIF
                     ALETYTFAAPTVGNESFVEHYNSVMINTNSPSHRVVNSKDLVPYGWAGLNNIIPDEIP
                     TFVPLPIGIVIIAVDLYLDSLGVKYKHVDNKVSLGTLTPTNCNSGNEEENYFCWVEFE
                     HSHNNYLRLLNADTVATSHRKSYGF"
     gene            59692..61446
                     /locus_tag="PLX80_00565"
     CDS             59692..61446
                     /locus_tag="PLX80_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19316.1"
                     /translation="MKNFILIILLTINLSAFCDEPYIVFPDVMNKVSILENTDGVQEK
                     KFYDADSLPVFPGFPKIINGQSFEGGIYCQMDSDSELEIVYGIGLSVQAWNIDGSNVT
                     GWPKSLSFNAQGAPSFGDIDGDGVEEIVIGTASLTGTTGSLFAFEKDGTPVTGFPVSI
                     GSARTVTLCDMDNNGSLEIITSKRLSSAGEIYIYKGDGTVFPGWPKAINHVPASSSAA
                     GDITGDGFPEIISESYTSIYAWDRNGNVLPGFPFILPNGDVNSYSSPVLADVDGDNIR
                     EIIFGSHVSSAGGLIYILKNNGTVLPGWPKSTNYWVYGPPVLGYLNDDNVIDIIVGDQ
                     IGSGTPVNFVYGWDKDGNVLSGFPIGPVNAVNNQVALADIDNDMRLELLIDDNSQTGG
                     IGKYPAFNHDGTPVMDWDLTVNGTSFFNMPCLTDLNSDGILDIVGAGFTLFGSNQTSV
                     YIWNAGIPYDAAKIVNPVFQFNVRHNGVYGDNNLVSVLNYNNTIPDNFKLEQNFPNPF
                     NPSTVINYSLPSEFKGNVKLNIYNALGSEIAELVNESQGPGNYSVEWEAVGFTSGIYF
                     YVLRSGNFKESKSMILLK"
     gene            complement(61586..63166)
                     /locus_tag="PLX80_00570"
     CDS             complement(61586..63166)
                     /locus_tag="PLX80_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY19317.1"
                     /translation="MQKIIISFLIFFSFHLLLRSQIVHEDWVQKFGSNTETINTLSGP
                     LIDKMGNVFISGTVSSSTLSDYVTIKYNSAGVLQWTKKFSFRIEDRVVDMALDNEGNV
                     CVTGLSENLTGTYDIITIKYNTDGDSLWGNRYNGETAFTMDQPVAMDIDNNNNVLVSG
                     YSFGSTPMTLVTIKYSPQGDSLWIKRFALGGTDIPKDIYADDNGNVYVYGRGTRIIKY
                     DTNGNELWNRIYQFSGSESNKVLTGDASGNIYFGAKKFTSTFDDFVIVKLDPDGDTLW
                     TKIRNGFGGLLTTHDDINAVCLDKDDNVLITGEIYNAGPVSFATLKYSSEGVFQWERN
                     YYDPLNGMGGKDIVCDALGNIYVTGGSIDFITLKYNSAGDSLWSIRYNGPANQVDFGS
                     SIAIDNNSNLYVTGRSRLGTGLSGYEAVTIKYTQTLTGISAEEIRSKDYYLNQNYPNP
                     FNPKTIISYDLKESGKLVSLKVYDVLGNEVATLINERQNSGSYKIEFDGSKLSSSIYF
                     YKLVTENYSEIKRMMLVK"
     assembly_gap    63332..63431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(63447..64877)
                     /locus_tag="PLX80_00575"
     CDS             complement(63447..64877)
                     /locus_tag="PLX80_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19318.1"
                     /translation="MQKTRLIQFLSSLNPSEFKNFRDFILSPVFNKNKFIKSLYDLLE
                     MYYPEFDDTDLTEENLFKKLFGDSKYDYYKMKNLVSDLLSLGKDFLVFQRFKTSDEIK
                     ESLLLSELERRNLDKIYEQSFKSATARLEKSRITDENYLFHKFTLTKELTNFYSPRKP
                     NENHHFQQDKLNLFVSYSVIMLLKTYNIMLHEINQNNYRYDMRMFEQVLSYIENNPEE
                     TNPTIKVYYQIIKLCLDKSEENYLKLKQLREEYNHELNSADRYMLFLHLDSYCATEYN
                     YNGRTDLLFDQHLLSKECNMNHFPELGKILYPDFLNDIKKAVRVNEFSFAEKYLNDFK
                     KNLTEEKENTLNFCYGFIAYGKKEFDLALELFSKTNFSNYIMKVQVKLLVIQLVYEKQ
                     FYDQVFLMIDSYRHYISREKMITEVLKNTLLNFLRVIGELTKLRTSSDLKDKEKVSKI
                     IYDINNLNSNHFGMKLWLIEKADEIK"
     gene            65147..>65493
                     /locus_tag="PLX80_00580"
     CDS             65147..>65493
                     /locus_tag="PLX80_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19319.1"
                     /translation="MILILALNVISSADVFSANEYFRSIATGNWNSTSTWEMSLNNST
                     WVPATTTPDATSNLITVRYPNVVTVTANVNADQFTIDSGTVSINSGITLTLLDGSSTD
                     FTILKGGTVTGSGT"
BASE COUNT        21957 a        10603 c        11252 g        21581 t
ORIGIN      
        1 gcttcatata tggaaaaaac tcacggaaag attgagattg gtatgaatgc agatataatt
       61 attttgaaag atgatctgta tgaaatgaat gcggaagaaa taaggaatgc gaaagtggag
      121 acgacatatt ttaatgggag aaggatttat tctatataga aataaatatt tgatgactta
      181 cttatttcat ttacaaaaca gattttttat tattaatggt taagttcctg tgaacagctg
      241 attttgttaa cgccgttatt actgaaattt aagatgcatt tacttttgag aagtgggggt
      301 ttttctgaaa taaaccgttt aaacggttta tatcaatcat aaaccaaaac ttatataagc
      361 tgctcaactc aaacattaaa aatggatctc cccaattgaa ctaccaggtc accaaatttt
      421 ttaacacaga aatccaatat ctcagttagt ctgtctcaat aattcacctg ccaccttata
      481 tgcattcagc atatcgatca caccaccgga ttttgataat ttcgaaaaat catatttcgt
      541 atttgaatct ttaactttta cttttagtcc gtcatatttt cttacagaag ataagatcgc
      601 ttcttttaac tgaaatgctg taatctgagg ataatatgat tttaataccg ctgctacacc
      661 cgttactgaa ggtgatgcca tacttgttcc gtctattgat ttatacttgc ctccgggaat
      721 tgtagagtag atctgaactc cgggtgcgaa tacatcaacc acctcatctg aataattact
      781 gaatggtgcc agtagatcga actttgcatt cttattttca ggatctttat ctttactcca
      841 ttttttcatc catgagctgg ctccgactac gatcatatta tcaaaatatt ttttctctcc
      901 gttttcttct gtatatttcc tcggataatt tactatgtct tttacatcat ctccttcatt
      961 tcctgctgat atcacaaaca gtactccctt ttcttctgca tatttaactg catctactac
     1021 atattcagga tacggcgaga aatattttcc cgctgacata tttattatac ttgcaccatt
     1081 atcaacagca taccggatcg catttcctac atccttatct ctttcatctc cttcattcgg
     1141 cactgctctg agaaacatta tcttcgcata aggagcatga cccgtctttc tcgaagcaat
     1201 tacaccagat acgtgagttc cgtgctcttc atctttctct gttacatcat tattcccata
     1261 attggtttca tctaatttta acggatcgtc accgattaat tcacttacat ctatcgaatc
     1321 aaaaagtact ttacttttta actcatactc atccgttact tttgcaagat catctttttc
     1381 cattccaaaa agaagatata ctccgagaat tattgaagca gcatcacttg tctttccttc
     1441 cggaagtgac attgaaatgc cctgtagttt ttttggatca gttgtaatat tattttttct
     1501 aaggacgtct tctgcagctt ccatttctat aagtgtttca tttaatcctt tatatgtagc
     1561 cataatttca tcctgctttt ctacaaattc ttcttccact tttataaaat atggatccga
     1621 aacaggtgtg ttcagttttt tcaatctaaa atattctctt gaaaattcat aaggaagatg
     1681 ttttgcattt cccaaaaaat tccatccgtt aaaatcatcc acatatccgt tttcatcatc
     1741 atcgattcca ctcactccat taacttcggc tgaattttgc cagacattat cattaagatc
     1801 gggatgatca atatcaaatc ccgaatctat cactgcaact attacatcac ttctcacagg
     1861 ttcaggggaa agtgttttaa gatatttata aaaagacttt gtacgggtac cttcatatcc
     1921 atcaatttca ggatcgagct gagtccagtc cggaagttct tttcccgggt ttttatgatt
     1981 attaagtaat tttaataatt ggccttcata atttttacta aatacttcga gatctatttt
     2041 tgaattattt tccggagagt caaaactaag gaatggaatt aaaacaaaca acattaacac
     2101 agacataaat aatctgttaa ctttactttg tattttcata taactaatag ctttaaattt
     2161 atatattgaa gtctaattta tgtattacgg ctaacattta taagtctcaa ataattacaa
     2221 tctatcaaca ttaccttatt aatccaatat gtctgaccat tatcaaatct atctgaaatt
     2281 caaaaaaaag atagatgata ttttatttaa catgatcaaa aatgaacagc cggtaacttt
     2341 atacgagccg atcaattatc ttctgagcag cgggggtaaa aggatccgtc caattcttgt
     2401 aattttttca tgcgaagcag caggtggtga tcctgataaa tcaataaatg ctgctgttgc
     2461 tgttgagctg ctgcacaact ttacactcgt acatgatgac attatggaca atgcagacac
     2521 ccgaagaaat ctccagacaa ttcataaaaa atggaatgag aatgtagcca ttcttaccgg
     2581 tgatcatttg ataggacttg cttacaaata tctttttaaa acagatactc aaagattaaa
     2641 ggaaacagca gattcgttta cagaaggcat tatagaagta tgcgaaggac aaagttatga
     2701 taaggaattt gaagacagaa atgatgtaac tcttgatgaa tatataatga tgataaataa
     2761 aaaaacagcg aagatgcttg aaacttccgc tgctatcggt gccttaaccg gtaatgttga
     2821 tactgaaatg ctgagtaatc tgaagaattt tgctttaaat atcggactgg cttttcagat
     2881 tcaggatgat atgcttgata ttatggctga tgaaaatgaa cttggaaaaa agattggtgg
     2941 tgatattctc gaaaggaaaa aaacttttct tttgataaaa gcaatagaga ctataagcaa
     3001 tgaaacagac aaaagactta taatggatat ctgtcaaaat tcagaacttg agatcacaaa
     3061 tgaaattatt gaaaaagtaa aagaagtata tattaaaaat gggatcatag aatctgcaaa
     3121 agctgagatc ataaattata ctttaaaagc tgatgaatat ctcaataaca taaaggacac
     3181 taattcgcgc aacaggttaa aatggttttc aggaatgctt atggaaagaa agttttaaaa
     3241 caaaattttg gatcagagtt agaataataa attacttaac cgccacaaca atataatgct
     3301 ggaaaaagaa gtaaccattt taaacaaagc gggaatgcat accagacctg cttctgcaat
     3361 agtaagaatt gctgcaaaat ataaagccga tttttttata tccaaagata actacgaagt
     3421 gaatggaaaa agtataattg gagtgatgac tcttgcagca gaacaaggct ctacactgat
     3481 cctgaggttt gaaggtgaag atgaggaatt gcttgctgat gaaatgctgg aattttttga
     3541 agacggtttc ggagaagtaa aatgaatgag caaattaagg agatcagaag tttagtcttt
     3601 cagtctgacc tttttaaaaa aatttctgag ataaaaaaac tcaattcaga gaaattttta
     3661 tttagaggaa ttcatggttc tctgatttcg ctggtaaata actacctcta caataacgta
     3721 tccaaaagaa taatttttat tactcatgac gatgacagac tttccaaaat aaaagacgat
     3781 actgaatttt tacttgagtc gggaaatatc acatcttaca caggaagacc aaatgaaagt
     3841 gatccggttt ctaatctgct gatgaacctc tcaggtcagg acgaattcat tatattcctt
     3901 catatcagcg gtcttgaaga cagaaccatc tcaaaagata aatttttatc ttcaataatc
     3961 gaaattaaga aaggcgaaga gttcagcttt gagaaactga ctgaacttat gttagaatat
     4021 aaatttgaaa aaaaagattt tgtagatgca gaaggtgatt tttctgtaag aggagggatc
     4081 gtagatattt tttctgaaaa tcatgatacg cctgtcagga ttgaattttt tggagatact
     4141 gtagaatcaa tcagagaatt tgatctgaat tctcagagat ctgtaaggga tgtagattct
     4201 ataaaaatag gaatgaattt atctaccgaa gaagatcttg aaatcccttt gactgaaaat
     4261 atcacagatt attttccgga tgatgcgatg ctgataattg atcaacctga aattactttg
     4321 agtgagatta aagatgaaaa aatattatcc attttagata aacataaaca aatacacata
     4381 actgcatttt caggaagtga cccgcaccaa agcagtgaaa ataattcatc agcagaaatt
     4441 aacattaact tcggttcaaa aacccagcct gattttcatt cgaatttaaa gctgctttat
     4501 cagaatctat atgaaaatat aaaaaatgga tacaaagcat ttattatgac ttccgatgaa
     4561 aggcaggctg acaggataaa agagctgata gaagactttg aagatgaatc tgtaatggaa
     4621 caggatgact ttattcatca tgaagatgca gattctgaca gatccaagat aactgttgac
     4681 aaaaatttta ctataaaaaa taaattcact gtcatacccg aatcctttca ttatggattt
     4741 atatatcagg aatcaaaaat acttatttat acagagcatc agatctttgg cagatatttt
     4801 aaacaaatta aaaagcggaa acagaaattc aaaggtctta ctttcaatga acttaaggaa
     4861 cttacttatg gagattatat agttcacaga gattttggta tcggagttta ttcagggtta
     4921 aaaaaaatta ctgtaggcaa tagtcatcag gaagtagccg tcctttccta taatggtaat
     4981 gataaacttt ttctgaatct gaacagcatt aacctgatca aaaaatattc aggatcagaa
     5041 ggtcatgtgc ctgtgctgac aaggcttggc ggaggtgaat gggataagct caaagcccgg
     5101 acaaagaaac aggtaatgga cattgcaaga gacctgatct tactttatgc aaaaagaaaa
     5161 tctgaaacgg gacataaatt ttcgtcagat actcattggc aaaaagagct tgaagcaaat
     5221 tttatgtatg aagatacacc tgaccaatac agcgctacag tagatacaaa gactgacatg
     5281 gaatcagaaa accccatgga cagactcgtt tgcggtgatg ttggatttgg taaaaccgaa
     5341 gtagccgtca gggctgcatt caaagcagtg ctcgaaaata aacaggttgc tattcttgta
     5401 ccaactacaa ttcttgctgt tcagcattac aatacattca gagacagact ggcatcattt
     5461 gctgtggaag tggataacat cacaagactc aagcctgcaa aagagcagaa gaaaattctt
     5521 gcaaaacttc tggaaggcaa actaaatatt ctcatcggta ctcacagaat actttcaaag
     5581 gatattgcat tcaaagatct cggacttttg ataattgatg aagagcagag attcggcgta
     5641 aaggcaaaag aaaagctaag aagtattaag ccaaatgtag atacattaac actaaccgca
     5701 actccgatcc cccgaacttt aaatttttct ctgcttggtg cgagagatct ttcaattatt
     5761 aatactcccc caaagaacag aaaaccgatc atcacagaga ttattaaact cgactggagt
     5821 aatattgcag ggctgatcag gacggaaatt aacagaagcg gacaggttta ttttgtaaat
     5881 gataagatta aaaatctaca tcaacttggt gatactataa agacttatgt ccctgaagca
     5941 aagatcggta ttgctcatgg tcagatggac ggtaaggaac ttgaggatat tgtgatcaga
     6001 tttattgaaa aaaaattaaa tgttttactt tgtacaaaga ttattgaatc cggtctggac
     6061 attccaaacg ttaatactat tataataaac aatgcagaca aattcggttt ggcagaattg
     6121 tatcagcttc gcggaagagt aggcagaagt gatgttcagg cattttctta tttgatagtt
     6181 cctcctgaag gcaagataac aaagactgcg gtcaagcggc ttcaggcgat tgaagaattt
     6241 actgatctcg gttcaggatt taatcttgca atgcgagaca tggagatcag aggtgtcggt
     6301 aatttactcg gcaaagctca gagcgggttt gtacaggaca tgggatttga tatgtacatc
     6361 agtattatag aagaagcagt tctcgaactt aaagaaaatg aattcaaaga tctttttaaa
     6421 agtgaggaat cacttaaaaa gatcaatgaa agtattgaaa agaaacttga ggaaaaacat
     6481 tctcttattg aaaatgatat caatgcattg atacctaagg attatataag caatgacaca
     6541 gagaggctta atatttacag aagactttat gaaataaatg atcagtcaga actggaaatg
     6601 atcagtctgg aactaagaga cagatttggt gattatctcg atgacgtgga aaatctgctg
     6661 aagataattg atcttaaaat tgcagctact aaactcggta ttgaaaaagt ggtaatcaga
     6721 gacaaaacta tttcaatcca ctttcctcag gataaagaca atatgatatt tgaaagcagt
     6781 ttttatagtg accttattga aagaatttcg aatgaccgtt caaggaaata tacaatatct
     6841 cctgaaaaag aaagactagt aatagaagta aacacaaata atacagatga taaagtaaga
     6901 ctgaccgaag caaaaaattt attaaaagga taattatgaa tattcataat tgataaacat
     6961 ttaaaacaaa aatttttaaa tttgaaaaaa agtaacaaac aaattaattt tattatgggt
     7021 aaaacaaaaa ttaactgccc taactgcggc gtagacattg atgtggacga agttttcaaa
     7081 cagaaagctg aggaagatat taaaaaggat tatgacgaaa aattatcccg tcaggtacag
     7141 cagttaaata taaagaaaga agaagttgat gctgagaagc taagagtcaa aagacttatt
     7201 gaagaacaag atgatatcat caggggaaag ttgattcagg aaagaggaaa gcttgttaaa
     7261 gaagccgagt ttgcagcaaa aagagagctt gagtttgaaa tggtgaaact caaacaggat
     7321 gttgatatta agtcgaaaga aaacaagcag tataaaatga aagaggtgga tctgcttaat
     7381 aaagaaagag agctgaaaga aaaagaagag catatgaaaa ttgacatgaa gaagatgatg
     7441 cttgaaaaag aggaagagat ccgtgaaaaa gctgtgaaag ctgagcagga aagaaatgag
     7501 ataagaatga tggaatatga cagagaatta gaagaacaga aaaaacagct ttcagaaatg
     7561 aagaaaaaag agattgagtt taagaataaa gaaaaagagc tgaaggacag tgaggaacag
     7621 ctcaggatcg atctcgaaaa acaaatgctc gacagagaaa aaatgataga ggaaaagatt
     7681 cagaaaagag agcaggaaaa gagtgagtta aagaaaatgg aatttgagaa aagattggaa
     7741 gatcagaata aagagctttc ggaaatgaaa gtaagggaag ttgaatttaa aaataaagaa
     7801 aaagagctta atgaaaaaga agaacagctt aaactggaaa tggaaaaaca aatggctgat
     7861 cagttaaaga tggttgagga aaagacaata aaaaaggagc aggaaaaaaa tgaattaaga
     7921 cagagagaat atgaaaagca gcttgatgat cagaaaaaaa tgatcgatga attaaagaaa
     7981 aaagctgagc agggttcaat gcagctccag ggtgaagttc aggaacttgc tatagaggat
     8041 tttctgaaga atacttttcc ttttgataag ataatagaag tgaagaaagg cgagaaaggt
     8101 gctgatgctg ttcagcttgt aataaataat cttcagcagg aatgcggcaa gattatttat
     8161 gaaagtaaga ggacaaaaaa tttcagtgaa gggtggattg aaaaacttaa agatgatcag
     8221 atcaaacagc aggctgacat tgctgtcata gtaactgaga ccatgccaaa ggatatggac
     8281 agattcggac agaagcaggg tatctggatc tgcagttttt ctgagataaa aagtctgtct
     8341 attgtatttc gcgagatgct tctcagaaca catgcagtaa gatcagtgaa tgtaaacaaa
     8401 ggcgacaaga tggaaatgct gtacagctat cttacgagca gtgaattcag acagcaggtc
     8461 gagacaatag tggatggatt ctcaaacatg cagacagatc tgaacagaga aaaaattgca
     8521 atggaaaaaa tatggagctc cagagaaaaa cagatccgaa aagttttgtt aagtacatct
     8581 tcgatgtttg gatcaataaa aggtctggcc ggaagttcga tagaagatat caaatcactg
     8641 gaaatgcctg acggagaata aaaaataccg cattatattt tttaaaaaat aatacggtat
     8701 tttagaatta cccggatcag aaagaataag atgtgccaat aaagaatctc agatcattct
     8761 gtggctgcag tccatataga ttttgtgtga ttggaagctg aatagtaaaa ttcagagaat
     8821 aatttgaata atatagatct agcccacctg ttccaaagaa tgcagtacct ccgctggaat
     8881 taacagaaac atcatcctgt gtatctcttt ctgcagtttc aaaatatatt cctgcatgag
     8941 gaatgaatgt aaaatctgta gcttcgaatt gataaaaacc tgaaaaagtt acgttaaact
     9001 gatttgcaaa tctgtaacca tttttgtttt caccgttaac ggtataaaat atatcagtac
     9061 caaatcctat accgggattt tcaaatttca taagatgact tgcggaaagc agcagatcaa
     9121 aggctcctgt acccggctga aaatgcgggt ctataattcc ataaaccaat tgcttattat
     9181 aaacaccggt aggaatttta actcccgcac ccataaaaaa tctctgccag aaatttgttt
     9241 cgcgaaccat atcagtatta taaagctgat actgagcaaa tacttttgca tctcccattc
     9301 cgttcaactg caatttatca atttcattaa acgagaaagg tatgttgaaa aataatctga
     9361 tctttggagt aatgtaaaat cttccgaata gttcaatgtt tctgtaatac tcggcatact
     9421 caactcccgg ttcgatttca tgatctgtct gaggttccga ttcgggaacg atcaattctc
     9481 catccgctcc aaaaaaatta aaagaactat atcttacacc gatctgattg tcactgtaat
     9541 tcggatcaag tcccataaag catccgcaca ggtcacaacc ggtttcctga acatcactcc
     9601 tgtcatttaa ttcatttata aaatttacat catttatatc agacagcctt ttaggagatg
     9661 atacttttaa gccggtgttt ttgcaaagac attcagcata aagttcagca gagctaatag
     9721 taaataacaa taatacagtt agaatatatt tcataattta ttattaattc gttatatcaa
     9781 aaaatgtatc atacgacagt gtatttttcg tcagtctgat ctttaatctc catgctccgc
     9841 tttggtcaag attcactaca cctttataat gtccgagcga agtatgagaa ggagtaacat
     9901 tatttccgct catatgtccg gttgacaaaa actcaggtgt agaagtgatt gtataagatc
     9961 cgtcataagg aaaaagttca ggttcatttt tgtgaattac gaattcaaaa tcattcagtc
    10021 cggtaaccgg ggttttcgga gaaatatacg aaacataaag ttttgtactg tcaggcatta
    10081 tgatagattt aaacctgtcc ggattatcca gaattcttgg tacttcaaat tcgacttcac
    10141 cttcaggctc tccctgagcc tgatgattat gcactctgaa attaaaatgc cagtgtagat
    10201 caccgttagg aaatgcctgg gttaaaacaa ttgctcccgg aaatttcccg tcaattgcag
    10261 tttcgcccgg attttctaca ggtgcggaat gtccatgatt caccggtaaa aattcaacat
    10321 gagaatttgt gatagagaaa tgtgtagctg agtcatataa tacaatataa attttattgt
    10381 aaccgagttt tagagaatcc tcagcataaa aagacattat agctgatgct ccgggcacat
    10441 aacctgaatc tatttttacc agatctactg ttacaggtgg atcaggagca acacttactg
    10501 tgtcatcact gcaggaataa taaactgtag caaataatac gattacaaat attgaggaaa
    10561 ttattcttat tttcatttta agaaattatt tttaattaat atataattaa cagtcaggaa
    10621 atattttctg cctgactaaa tcgataaatt taaaataaga ttaagatttt ggtggatcga
    10681 actttaactg cgggtacccg gaagaatatt tttgagaatt atttattaat tgatcggaaa
    10741 tatttattgg actgtttaat attgtaacac ctgaaactat aaactctgag acctttaatt
    10801 tcatttcttt tacgggtgta ttactttttg catctgaatc attctcttca tttatttttt
    10861 tttcaagaaa acactttgct tcacagctgt catttttttt ctcacagaca gattctacaa
    10921 gctctttttt atttattgaa taaagagaat agtataatcc tataaaattg atatgtaaaa
    10981 acaaaatcaa tgaaagtgaa atacacgtta tatttttaag aatttgtctc aagtcaaatt
    11041 aaattggctg taagtttaga atattcggat tttaatgcaa gtcatttttc agaatcagca
    11101 tttttttcga aatcaatcga tacagaatta atacaaaatc tagcaccttc cggacccgga
    11161 ccatcatcaa atacatgtcc cagatgagca tcgcattttg cgcatctgac ttctgtcctt
    11221 accatgccgt ggcttgtatc ttcttcataa attactgagt cctcattcaa aggctcggta
    11281 tagcttggcc agccgcatcc ggaatcgaac ttggctttag agtcaaacag aagatttccg
    11341 cagcagatgc atttgtaaat accgtcatca tccaatgtat aatactttcc ggtgaatggt
    11401 ctttctgtac ccttctctct ggttacatga tactgctccg gagtcagctc ctttttccag
    11461 tcttcttctg atttattgat cttttctgtc atacattaat aagttataat tgtttgattg
    11521 ataatttata atatataata taatttagaa atattaattt tatgcttaat ttttggacag
    11581 agaaaaaaaa tcgattacaa ctgatgcaaa tcagctttca cataatattt ataatgacaa
    11641 agaatttgct gaaagctatt caaataaaat tgaatataat tctcataatg cattatatga
    11701 aagaccggca gttgtttctt tgatcggtga tcctgttggg aaaaaaattc ttgatgcagg
    11761 atgcggaccc ggcgtactgg cttcttttct tgcagataaa ggagctttca tcactgctgt
    11821 agattacagc agcgaaatga tccggctcac aaatgacaga gtagaaaata aagcccgtgt
    11881 cttagaatta aatctgaacg aaccacttga tgtatttaaa gatgaagaat ttgatataat
    11941 tgtttcttct cttactattc attatctcaa aaacctgaga ctttttttaa gtgaatgtaa
    12001 cagaatttta aaagaagatg gctgtatggt attttctact catcatccct ttatggattt
    12061 ttcatttcat tcggagggaa attattttga aactgaatta ctgacagatg aatggccgtc
    12121 atataatatt aaaatggatt tttacagaag gtctttgagt gatattttta atctaataat
    12181 ggaatcagaa tttgtgatag aagagttagc tgaacctatg cctgtatccg aatgcaaaga
    12241 aaaatttccg gatgcttatg agacacttac tcataagcca tggttcataa tttttaaaat
    12301 aaaaaaacag cagatataat gagagcaggt tatttacagt tcagaccaaa attcggtgat
    12361 gtagatttta acattaaaaa aattaattca cttatcggaa aaaataaatt tgatctattg
    12421 gttcttcccg aacttgcaaa ttcaggttat cttttctctg ataaaaaaga actggccgag
    12481 ctttctgaag aaataccaaa tggaaaattc tgtaaagcat tacaggaaat ttgcagtaaa
    12541 aataaatgct atatagtttc aggtatttgt gaagtatccg gcaacaaatt ttacaattca
    12601 tctatactcg tttatccgga tggaaagata gtgacataca gaaaaattca tttattcaat
    12661 actgaaaaaa aatggtttac acccggtgat cttccgctaa gatcaaatga tattactttg
    12721 aagagttcgg aaaaagtaaa gatcggaatg atgatatgct ttgactggat atttcctgaa
    12781 acagcacgta cactggcttt acagggagta cagatattgt gtcatccgtc aaatcttgta
    12841 atgcagtact gccagcgcgc tatgtttaca agagcactcg aaaatcatat ctttactatc
    12901 acggcaaaca ggattgggaa agacattaag aaagacggtg aaatattttt cacaggtcag
    12961 agtgtgatcg tttcaccaaa aggagaatac attcatacgg gttctaaaaa taaagaggaa
    13021 tgtttgatcg ttaacattga tccaaaaatt gcttctgata aatttatcaa tcctgaaaac
    13081 aatctttaca aagacaggag aaagaaattt tacaaaattc attaataatt tttaattttt
    13141 aatttttaat tttgcgggat ctgatattat tgcacggtgc aattggttcc agtgtacagc
    13201 ttgagaaact tgcagacagt cttaaagaca atttcagaat tactttactg aatttttccg
    13261 gtcacggagg gaaggaaatt ccgaaagatc cgttcagtat aaaattattt gcagaagatg
    13321 ttttatactt actggataag ggaaaatttg aaaagccgga tgtctttgga tacagtatgg
    13381 gcggatatgt tgcgatttat attgcaagat attttcccga taagctgaac aggatattta
    13441 cacttgcggc gaagtttgac tggaatccgg aaagttcttc taaggaatcc aaaatgctga
    13501 acccggaaaa gttaatggaa aaacttccgg attttgcaaa agaattatcg gatagacata
    13561 tgcctgctga ctggaaaata gttttgcaaa agacagcgga gatgatgatc aatttaggta
    13621 acggaaatag tttatccaaa gaggatcttt ccaaaataaa aaatgtagtg aggatcagtg
    13681 tcggtgacag agataaaatg gtgagtattg aagagagtat ggaagcgtac aggtgtctgg
    13741 ataacggaag ttttttggtg atgcctgata ctccgcatcc gatagaaaag gtttcggtcg
    13801 aaagactttc cggagaaatt tcagagttct tttcggattt ataaattatt atagtatttt
    13861 aaaaatatga agacacaatc aaaagataca agccctgaat ttgagaagat cttaattgag
    13921 aagttaagaa agaagagtac tgccgaaaat ctgagaactg ctttatctct ttcttcgctt
    13981 actattcagt tatcaaagag agctatacta agagcaaatc ccggcaaaag taaaagagca
    14041 cttgatctga tttttgtaga agctcattat gggaaaaaac atgcggataa acttgaagaa
    14101 tatttaaata aaatcggcga tgaatctaaa tgatatttag tgatatttaa ttatggtgaa
    14161 aactcattca taattttcta attcgttcat atacaatttt caagttttat tggttttaac
    14221 ttactttctt ttgcccgacc aaaagaaagt aacaaagaaa agtcgcccgc ggtgtaaaat
    14281 taccgaaatt tatttcgtaa ttgctataaa atttccaact cgaaatttga aaaatacaaa
    14341 tttctcaaac aaggaaattt tattttagca attactcata aatttttatc ggtaatttta
    14401 cacaggcggg ttttataatt gtaaaataat attctaatat aaaaaaaatt aattttagtt
    14461 accaatttat ctcatgtata cttttatatt tacataaaat gattgtttat tcagaaaatt
    14521 taaaaaacat aactcccgaa atgcttgaag gattttttgc cggatggaaa aaatatccgg
    14581 ataagaataa acatcttgag attcttaaga acagtgactt tatagtgatc gcaatggatg
    14641 acaacaaagt cattggattt attaacgcag taagcgacaa agttctttcg gcatatattc
    14701 ctttacttga agtattacct gaatatcagg gaagaggcat tggtactgaa cttgtaagaa
    14761 ggatgaaaat attactaaaa gatttttaca tgatagatgt ggtgtgtgat gaggagcagc
    14821 aaaagtttta taagaaattt ggattcaaaa aattcagttc tatggtgatc aggaattacg
    14881 agaatcaaaa aggcattcaa aataaataag aaatgataat tgaaattgac agtgaacttc
    14941 atctggaact attaaatgaa tctcacgctc cggagatatt tgaactaacc gaaagaaaca
    15001 gactgtatct cgacaagtgg ctgccatggg taaaatatac aaagtcaaaa gaggatactt
    15061 tgatgtttat aaaaaattcc actgagcagt ataaaaacaa aaccggcatg cagacagtga
    15121 tcaaatataa agataaaatt acaggattga tcggtcttgt agaaatcagt tatgtaagaa
    15181 atgtgaccga gatcggctac tggcttggag aggaatatat gggaaaagga attatgacca
    15241 tggcatgcaa agcgcttact gatcatactt ttgataattt taatgtcaaa agaatcaaca
    15301 taatctgcga tgtaaataat actgccagtc agaatgtacc ggcgagagtc ggatatttta
    15361 atcaggggat tctgaagaga gataattttc taaatggaga atatatagat cattatcatt
    15421 ttgtgatgcg cagacatgaa tgggagaaat agttaatagt taatagttaa tagttaaaag
    15481 ttaataaatt ataaaaaata aaattttata attacatttt aaattgaaaa ttatacaatg
    15541 cgcattatac attgcaaatt gaacattgaa cactgcaaat tgaacattga acactgtaat
    15601 ttgaacatta taaattgcac attgaacatt gaaaacatcc acactgtcat tcacgcgaaa
    15661 gtggggggag gtaccttttg ggtcatctcc gaatgtaata cttagccaac attcccataa
    15721 ttcctcattc aaaattcgta attccgaatt gaaccctgtt ccgtgtcttg actttaaaaa
    15781 atatagtatt tactttctaa aaaaattaat aaaggagaaa ttgtgaaaaa cctaattgct
    15841 acctttcaat ctgcttgctt gcttgcttgc tttagtctta taaaccctgc tcaatccctt
    15901 gctcagctta cttacccgac acgttatcca acagcagtga ttcctaacaa cggtgaaggc
    15961 aatagactat ttttccttta tatgccaata atctatgagg ataatgttgg tcaagatact
    16021 tttaaaatgt attatgtaag agtaagaaag gaaaataata ttactaaagt acaccattat
    16081 ctcgctcgat ctacaaatta tggaataact tggacagata ctcaatcagt atcagtttta
    16141 tcacatgaaa caataatcta caatcctgat tcttcaaaac attatctatc ttttggaaaa
    16201 tggactacag gtcttaacaa gaaatcttat atggttaaat ctcaatcaat tgatggagga
    16261 ttcaatttta caggaagtga tagtgttatg gaacttggcg aagataaatc ttttatctgg
    16321 aatgaagaag aacatgaata ttggggttat gttcgacctt atcatattga accatcttgt
    16381 tgtaatggag aagatccacc aacttgcgtt tcatttggaa ctggagtcag gaaaatagca
    16441 ttaatgaaaa acgatggata ttttggaagt tcttcagcct ggagtgcaag aaatgtaatc
    16501 cttgaagttg atactactga ttattattta aacagtactt cacctgatta caggactcaa
    16561 atatattaca tgcaggtttt cagaaatggt aaagactggt ggggattaat tggaatgtac
    16621 agagttggag ataatggtgg tgaaaacaat cctcatccct catttgcaaa tactttacaa
    16681 tatacagttg atgttgaatt agtctggtct gataatggag tggactggtg cagaacaaat
    16741 aacaggcagc cgattatagc attacatgat tcaataaagc agatatttgc cgttggtacg
    16801 gtagttcagg attcagtata tttttattca attgaaagta ctttgcacca ttctcaatat
    16861 tatactacca attattgctc atatccaaac gatactataa agaatggaaa atattttagt
    16921 atatatttat ctaaaatgag tttagatgat ttaaatgaat ggcgtccttc ttcaaatgtt
    16981 aatatatatt gtgcagtcga gggcttttta aacaccggaa ccggaaaaca taatttaagg
    17041 gacacattaa ctgctgaatt aagaaattcc acttcacctt acggattagc cgcctcaata
    17101 aagtctgtca tagattccgt aacttttctg ggaaatttca attttccaca tgtagatccg
    17161 ggaaattatt atttggctat atatggaaga aattcacttg aaacatggag tgatagtgcc
    17221 atttctgttt gtaacggaat tgatgctgaa cataatttta taacaagtta ctttgaagct
    17281 tatggcggaa atgttgttct taaaagcgga atttattgta ttttctcagg agatgttaat
    17341 aatgatgatg ccattgatct tacagatctt tctttaatag ataatgatat ctctaattct
    17401 gtttcaggat atgtaaattc agatttaaat ggagatggag aagtagatat tgcagatgca
    17461 acaatcgcag ataataactc aaataattca gtaagcacaa ttcatcctta aattactttt
    17521 taaaaaaatt tataaataaa aatatcatga aaaactttac aaaaataatt ttagttacaa
    17581 tattttcctt attgacttca tctgaaatat tctccacacc aattaaccag ttcataaatt
    17641 ccgtctcccc tcccttgaac gcaaacagtg ttatcaaatc gtccgacatt gttatcacat
    17701 ttgaacaagt catgaacggt tcgactatga taaatgataa tgttaaagtc tttggatatc
    17761 agactgggct gatgaatgtg tctttggatt ttaattcagt tgaaaatact ttgactatca
    17821 atcctttaaa tcagtttaaa aatggcgaga taataagtgt gacacttact gcaggtatta
    17881 aaaccatttc caatcaaaat atttctccgc atgtatttaa atttcgagca aaagcgcttg
    17941 gaggtacagg cagttttaac ttatcatcgg aaataagcaa tactgttgat ggatacatca
    18001 gatcaggaga catagacagg gatggagata ttgatctggt tataaataat gctgtttata
    18061 tgaataacgg aaatgcattt tttaatatct ctgaatccct aaaccatcag ggaagaccgg
    18121 atatgtttga tgcggataat gatggtgatc ttgatatatt aataagtaat aatggtactt
    18181 tttattatta caaaaatgac ggtgttggaa attttctttt attctctgta aacagtggca
    18241 gtatttgggg ttttggtgat ttatcaggaa atggatatat agatatagca tattatataa
    18301 atactcggga attaatgact aaaatgaata caaacggaat tttcaatccc ggtatgtcgt
    18361 acaatttacc cggtcaatgt aatatcaatg aaaattacta tgataatctt ttgtttgatg
    18421 atttcaacaa tgacggtgga gttgacatta tttcaatcag cggatgggtt ggtggtgtaa
    18481 gtattactgg atatgattta tgcagaaaat ataatcaatt aacaaatgac ggaataggtg
    18541 atttctcagt gcagacagta tttaataatt atatagaagg agcagcacca tttatttttg
    18601 actcatttga ttctaagtca tttgacgtaa acgaagatgg acttgtagat atagcatccc
    18661 catatattga aattaggaac aatggaaatg gatcttatac tcaaattgga gatttcggtt
    18721 ttcctttcag caattcattg gattttgatg caaatgggaa tggttttata gatctttgtg
    18781 taaattttaa cggagcatta atgtgtttca ataacaatgg aactggtgta tttactttaa
    18841 ctgttgcaat aaataattta aacttttcat ccggttctgc ttcaggtgac tttgataatg
    18901 acggagatat tgacattgct ttcaaagagt ataacaacga caaagtcgcc atcctcctca
    18961 acggtgattc accattgcct gtcgaacttt catcattcac ttcatcaata aatcaaaatt
    19021 cagtaaaatt caactggact acttctcagg agcagaataa ttcaggcttt gaaatcgaaa
    19081 ggtctgataa ttcatctaac gttcagggtg tatggaaaaa aattgatttc ataaacggcg
    19141 ttggaaattc caatactcaa aacaattatt cattcgaaga taaaaacctg acttcgggaa
    19201 aatataagta ccggctgaaa cagatcgact tcaacggcag ctttgaatat catgaacttt
    19261 caactgaagt cgtgatcggt attccttctt ccaccgaact gatgcagaac tatccgaatc
    19321 catttaatcc tgttacaaat atttcttaca gactttcgga gaatggattt gttacattaa
    19381 gagtttttga caattccggc agagaagtga agacgctttt gaatgaattt aaagaagcag
    19441 gatattttac aactgagttc aatggcagtg atcttgcaag cggtatatat ttttataagc
    19501 tgaccgcggg taattttgtg cagacaaaaa agctgtcgct ggttaaataa ttgtaaattg
    19561 taaattataa attacaaatt cttaaacttc ggaagtttca ttagcttccg gagtttttat
    19621 tttggttcac aatcaagtaa tccctttatc ctttaatcaa ggttaagact ctccggcatg
    19681 gattcctgca tgcgcaggaa tgacaaagtt ttgcggactt cggatgtttg tgttattgaa
    19741 cattcgagtc aaatcaatca gctccattct taaaagaccc gcttactaag actgagatga
    19801 cccagagtgt acttcccgat tttgcctgta tgaaactagg gctgtaatga ctcacagaag
    19861 tctgaaaata actcaaagat ctccgtaact ttctactatg tatccgggaa taacctgccg
    19921 agatccgtaa ctttctcaaa gagttccttg acaaacctac cgatttccca gaacaactca
    19981 cggagtccgt aacttgctca cggggacccg gaaatttctc acggagtacc gtaagtttct
    20041 cacagtgttc aggaaatttc tcacggagat ccgtaacttt ctcaaggaga ccggtgaata
    20101 gttcatggag ttccgtaact tgctccagga tttccttgac aaacctacgg atttccttga
    20161 aagatctacg gagccagttt ttttaataat ttgaacctgt agtgccaaaa ttagacaatt
    20221 ttaacatttt ttaaaattgc tgatgaatat atttttaaat gatagaatca actgacatca
    20281 tcgcagagat gccggtgata aaatagtttg tgaaaaattt gattccaaaa ttaagaatta
    20341 taaaacaatt tatgtgacac aatcacctgc caaaaaattt tttatcaagt tgcgtgtctt
    20401 gactttaaaa attaatgtat ttacatttta aaaaaattaa ttaaggagaa attttgaaaa
    20461 acctaattgt tacctttaaa tctgcttgcc tgcttaagtt tcatcatacc tttaaattct
    20521 ttcagtacat tacatatact catttttaat aataaattaa ctcaggagaa attatgaaac
    20581 agctaatctt tacaacttat atattaatga ttggaataat atcatttaat tccggtctgt
    20641 cttcacctat caatccatac ataaactcag tatctccacc gatgaacgca aacagcgtag
    20701 tcaaaacttc aaacattgta atcacatttg aacagacaat gaacggctca acaatgacag
    20761 atgcaaacat aaaactattc ggatatcaga ccgggcttat gactgcttcg cttgattata
    20821 actcagtaac aaaaactctg acaattaatc ctgttaataa ttttaagaac ggcgagaaga
    20881 tcagtctaac acttacttcc ggaataaaga caattgctaa tgagagtatt tctccatttg
    20941 tttattcatt caaaacaaaa gcacttggcg gtacgggaat ttttacaaaa tcatctggaa
    21001 taccaactat tcaagttcca tatataatat ccggtgatgt agataaagat ggtgatcttg
    21061 accttctgat atcagaaaag atttataaaa acaacggaaa cgctgtattt acattttact
    21121 cttcaattag tgtttctgga tatccggtta tggctgattt tgacaatgac ggagacctgg
    21181 atattcagga aacttcaaat aatattacat atttttttca aaataatggg aatggagatt
    21241 ttattcagac tacttcttat ccaggattga atgggtcaat tggagatttg aatggtgacg
    21301 gatattttga tatggtttat ttttcaaatc aaaataatac tgatgtatat attgtaaaaa
    21361 attctaatgg tatatcttca attgatacaa cttatcaaat aaatcattcc tgttttaatc
    21421 cgcaatatgt ttattctgac agagtaatga tagatgattt aaataatgac ggtacgttag
    21481 atttagttgc aattaatgga cttggatttg caggtcctaa cataggattt tatagtcttt
    21541 gcaagacata tcatagtttt acaaataatg gtataggaag attttcagtt caaacatttt
    21601 attcacaaat attaagtgat tattcacccg aagttttatt tgtgggaggc tcaaaaactt
    21661 ttgattatga caatgatgga tttaatgata tgatctcacc gggattaaat ttaaaaaatg
    21721 acggaacagg attgcatata gatagaggat attttaacat ttacaataat tccttagatg
    21781 ctgactttaa tggagatgga tatattgatt taataaatat ttttggagca gcgtcatatt
    21841 tgattcaata tataaataat ggagatggtt cttttactcg aaatattctt tttattaatt
    21901 catattcatt cgtatcatca gcatcaggtg attttgataa tgatggagat attgatatag
    21961 ctgaatatga aaactatgga aatgaagttg caattttact caatggcgat tcgcccttac
    22021 ccgtcgaact tacttctttc acatcaagtg taatttcaaa ctcagttaaa ctaaactggt
    22081 ccacatccaa tgaacagaac aactccggtt ttgagataca gaaatcatta gtaaaaaata
    22141 aaactcctga ggcatggtct aagataggat ttgaagaagg tcagggaaat tctagttcat
    22201 ctgcagatta cagtttcgaa gataaaaatc tgtcctccgg aaaatataag taccgcctga
    22261 agcagatcga cttcaacggt aattttgaat tttatgatct ctcgaatgaa gtagtgatag
    22321 gcattcccgc ttctacagaa ttgatgcaaa actatccgaa tccatttaat cctgttacaa
    22381 atatttctta cagactttct gagaatagct ttgttacaat taaagttttt gacaattccg
    22441 gcagagaagt gaagacgctt ttgaatgaat ttaaagaagc aggatatttt acaactgagt
    22501 tcaatggcag tgatcttgca agcggtatat atttttataa gctgaccgcg ggtaattttg
    22561 tacagacaaa aaagctgtcg ctggttaagt aattgagaag caatttcaaa ttacaaatgt
    22621 caaatgtcaa atagtgagcc ggacatacga attcggagaa tttctatatc gagagacgtt
    22681 cccaatcggg agacgttccc aatcgagaga cgttcccaat cgggagattg ggaacgagtg
    22741 aaatcccaca taaatgtgat gattcttaaa gtttagataa ttactttgtt tcatcttagt
    22801 aagcctcttt ttgatttata aaaatatttc ggaaaaataa atttacaaaa caattttgag
    22861 gcttactact ttaaacataa tacctcgcga aagcgggaat ctataaaaaa tatgcgattg
    22921 ctgaattaat aaaattttaa tttttatact tttcaattat tgaaattggt ataatttttt
    22981 taccataaga gttggataag tttttcattt ggagattccc tcttttgcgg gaatgacact
    23041 aagggtagga atgacaaagt tatgttggct tcgtaactgt aaatcttcgg gtcttcgagc
    23101 ctttgaggta aataggttca ctgagttatt ttaaagggaa taacaaaatt tgggaattac
    23161 atgtactcga gcttcacatc atcagtcagc ggatgtgact gacaggtcag gatgtagcct
    23221 tcatcaattt ccgaatcaga aagtccttcc cgctcatcca tttttacttt tccggaataa
    23281 agttttgccc tgcaggttgt gcagatcccg cttcggcatg agaatggcgg gtcaagttcc
    23341 tgtgcaatga ctgcatcaag tattacagat ccttcgggta cgggaacatc atattcactg
    23401 tcattcagta ttactttaat atttctgtct ttaataattt cttctgaaac ttcatcggta
    23461 tcttctgaaa ctgtaacggg atccggactt accggggcat caaaatattc gatgtgaatt
    23521 ttttcctttg aataattttt tccggagaga tgttttttta ccagctccat catttctccc
    23581 ggaccacata taaagaattc tttttcgatc tgaactttat tgtccgcatc aattaaaatt
    23641 ttatcaagat cggttgtgtt gataaatccc ttcatcccat tccattcggt acctgattcc
    23701 attacagtat aatatagttt aaatctgtca ttgtgtttgt tttgaagttc atccagttca
    23761 ttcttaaaca ttatactgtc ctgagaatta ttaccataat aaagaatcac gtgactttcc
    23821 ggctctgcat aaagtactga tttgatgatt gagatcacag gagtaatacc gcttccgcct
    23881 gcgatcatca catacatttt tttattgtta ggattcagtt cggtaaaaaa tttcccctgc
    23941 ggcggatatg acatgagcgt atcgcctact tttaaatcat tgtaaaaata atttgataca
    24001 attccatcct ttacattttt tgatgctatc tcataagtat tttcagaata aggtgagctg
    24061 cacaatgaat attcacgtct gtattccttt ccgtcaatag tcaattgaac agacaaaaac
    24121 tgtccatgtt tgtattcgaa aatttctttt aaagatgaag gaatttcaag ctctattttc
    24181 acagcttctt tatttagaaa agtttttttt gagactttaa gttcgtatgg ttttatggac
    24241 atagattatt atgagatatt gattggtttg tagaaattaa taaataattg aatagtaaga
    24301 tcggaaaagt cttcttacaa aaagccaagc tcgagctttg ctacttcact catcatactc
    24361 tgaccccagg gaggatcgaa tgtgagtacg actttacttt catttaccat aggatgctct
    24421 ctgaccttct gctcgatctc tccgggaaga attcccgcaa ccggacaaga cggtgatgtc
    24481 agggtcatgg tgattagtac atcaccattt gtagcaggga caatatcata gatcaatcca
    24541 agctcccaca cgtcaaccgg aatttcagga tcataacatg ttttaagaac tttaattact
    24601 tcctctttta ttgtcgctat ctttaatctt tctttttctt ttatgctttc ttcagtttct
    24661 atttcctctt taaattcgac aacctttaca tttccattgt catctatttc cagatctttt
    24721 ttaatttctt tcggatcagt accaatatct ttattttcct gtgaagttgc tacttcagga
    24781 ttcatatcat tttcaatatt tccggtacca ttcgtatttt ttaaatcctt tttttcttct
    24841 ttcgaaaaaa gctttttcaa tttagttaac attttgtcct gaataagtta aagcaaattt
    24901 tttcatgtag tttatcattg atgtcaatcc gtttgccctg tttggtgaaa gatgttcttt
    24961 cagtccgatc tcatttataa aattcatatc agcatttaca atatctgcag gtttatttcc
    25021 tgataaaact tttatcagaa gtgagataat tccttttgta attacagcat cactgtcagc
    25081 tttgaaatac agtaatccgt ctttcatctc agcattcagc cagactttcg actgacatcc
    25141 ttttacttcg tatgattcat ttttaaattt ctcatcgatc ataggaagat tttttccaag
    25201 ctctattatg tattcatact tcatcatcca gtcttcaaac aaactgaaat cttctattat
    25261 actttcttct ctttctttta tattcatatc ttttgtcata ataacattgt ctttactttt
    25321 tccaaacctt ttgccagctc atctatttct tccaaagtat tatatacagc gaatgaagct
    25381 cttacagttc ccggcaattt aaatctctcc tcaataagcg gctgtgtgca gtgaaatcct
    25441 gtccttactg ctatgccgag attgtcgagg atcgaacctg tgtcataagg gtgaattcca
    25501 tccattacaa atgagatcac acttgcttta ttttttgctg ttccgattat tctgattcct
    25561 ccgatgtctg aaaataattt attcgcatga tcaagaagtt ccatttcagc tttatgaata
    25621 ttttcatgtc cgagatcttc tataaaatca actgccgctc cgagtcctat tgctcctgaa
    25681 atatccggtg taccggcttc aaatttataa ggcagtacat taaattcagt tttatcatat
    25741 gaaactgatt tgatcatttc accgccgtac tgatatggcg gcattttctc aagatgtttt
    25801 tctttaccgt agagaattcc tatgccagtt ggaccgtaca ttttatgtcc tgaaaatgca
    25861 atgaaatcac agtcaagatc tttaacatct attttctgat gaggagctgc ctgtgctgca
    25921 tcaaggatca caggtatatt ttgtgaatga gctgtctcaa taatttcctt taccggattt
    25981 attgtaccaa gtgaatttga aatataaacg acagaaatta attttgtctt ctcatttaac
    26041 atctctttaa attcatctaa aatcagttca cctttttcat taacaggaat gtatttcagc
    26101 agagcttttt tctgactgca aagattctgc catgggacca tgtttgaatg atgttccatt
    26161 tgagaaataa tgatctcgtc accttcttca atgatcatat ttccaaaaga agaagcgact
    26221 agatttattc cttctgttgt accttttaca aaaactattt cgccggaact ttccgcatta
    26281 ataaatctgc ggatcttttc acgggaattt tcatatgctt cagttgctct ctcacttaaa
    26341 gtatgaatgc ctctatgaac gttactgttg atagtttcat aatattccga gactgcattt
    26401 attactgaat taggtttctg agaagttgcg gcattgtcaa aataaataag aggctttccg
    26461 tttattgtct gatcaaggat gggaaattct tttcggagat taataacatt tatcttttca
    26521 atatttaata ccgatgagtt caatttttat ttcagttgtt cagaaagttt tacttcaagt
    26581 ttttcttcta tcaaattcct aaattcttca attctgatac tgtctaatac atcactgaca
    26641 aatgccttta gtaaaagttt ctttgcatca ttatcaccaa tcccacggga tttaatatag
    26701 aacatttcat tttcattaag ctgaccggta gtagcgccgt gactgcattt cacgtcatca
    26761 gcaaatattt caagctgagg ctttgagtaa atagtcgcat catcagacag taaaatattt
    26821 ccgtttgact gataagcatt tgtcttttgc gcatcttccc ttaccattat ttttccgtta
    26881 aagacacctg tagcttgtcc gccgattact cctttgtata attcattgct gaaacagtta
    26941 ggcattttat gatcggcaag ggtatggtta tcgacatgtt gctttccgga taaaagatat
    27001 aatccaaaga aatgtgattc agtattttgt ccgtcaaaaa gtgcattcag atcatttcgt
    27061 atgatcgaac cgccccatga tattgtagta tttgagtaat tgctgtcctg ctcctgatgt
    27121 acctgtgtca ttccgacatg ataactttta ctgtcttcat tctgaatttt gtaatgacct
    27181 gtcatcgaat tctttccgca gaaaatttca gtcaccatgt ttgagaaact gtaattatca
    27241 cctttggaat aatagtcttc tgctatttca gctttacttc cttcttcaat aataaataaa
    27301 tttctcggct gagacattac attttctttt tcagaatcac ttatgtgaag aatatgtaca
    27361 ggctctttta actcagtatt ttttttcaca taaataaatg caccgtctcc tgaaaaagct
    27421 gtattcaaag ctacaagact tctgtttttg aaatcagcat atttagaaaa atgcttttca
    27481 aagatctcag tatattgttt tttaccttcc tgaatattac ctatgaaaat gttttcacaa
    27541 tttgaaatta cagaagataa ttcactgctg taaattccat ttacgaaaac cagtaagtta
    27601 atattttctt ttgcaaataa atatttttca atatcagatg attttagttc ggatgaattt
    27661 tcaaaagtgt aatgaaaatc atgatctgtc agaggtttta gattagtgta tttccactct
    27721 tccattcttt tgacaggaaa tccaagatct tcaaatacag caattgcatc ttttcttatc
    27781 tgatgataat cacttgaagc ctctccgttt agactggatt cgaatttttg aaattcattt
    27841 ataagctttt ttttgagatc tgtaacattt gaatctttta aatctataat attttctttt
    27901 aaattatttt ccatttgatt aaatagtttt tccatctgaa tttatgcttc agcgttttcc
    27961 ttaatttcgt ttataaattc ataaccgttt tcttcaagtt ccaaagcaag ttctttaccg
    28021 ccggatttaa cgatctttcc gtcagaaatc acatgcacaa aatccggaac gatataattc
    28081 agaagtctct gataatgtgt aattactatg aatgcattgt tttcagactt aagctgattc
    28141 actccgtttg caacaatttt caaagcatcg atatcgagac ctgaatctgt ctcatcaaga
    28201 atagataatt tcggctcaag cattgcaagt tgaaatattt cgttccgctt tttctcgcca
    28261 cctgagaatc caacattaac atatctgctg agcattgcag gatctatctc tacaagttta
    28321 gctttctctt tcatcatttt cagaaagtct accgaactca tcggctcttc tccttttgat
    28381 tttctgatct cattaattgc agtcttcaga aaattcacat ttgaaactcc cggaatttct
    28441 attggatatt gaaatgcaag aaatatacct tcacgggctc tttcttcagg gttaagttcg
    28501 agaagatctt taccgtcata aataacttcc ccttcagtta catcatagcc ttcgcgacct
    28561 gtgagtacag aagcgagtgt actttttccg gaaccgttgg gtcccatgat cgcatgtatc
    28621 tcacccggat ttactgtcag attaattcct ttaaggatct gcttatctcc ttcggaagtt
    28681 tctatctttg cttttaagtt ttttatcgtt aacatattgt ttggttttta cctctaagac
    28741 tcaaaggctc taagacttaa taggttgtaa attttaaatt atttttatta aattataaat
    28801 ctttttattc cgtttttaat caaaggaaca ttaaaattaa ttaacatacc taaatgcttt
    28861 tttgataatt ttaaataact taatatctga gcatcaaaaa ccggattatg ttcatgaact
    28921 gccttcaatt cacaaataat tttatcttcc actaaaacat caattctaaa acctttttcg
    28981 aatttcaatt catcataaat aattggcacg cttacctgag aagaaaactc aatacctctt
    29041 tttcttaatt cataacaaaa acaaacctca tatattcttt catacaaacc tggtcctaaa
    29101 tttatatgaa cctggtatgc acaatcaaca attattttta aaagatattc ttccttttcg
    29161 gatggataat atttttcatt cataatttca aatcaataat attcataaat aatcttttct
    29221 ttgagtctta gagtctttga ggtggttatt agtgcttatt accccacact tccttctagt
    29281 gaaattgcga gaagcttttg tgcttccact gcaaattcca tcggaagttg attcaatact
    29341 tctttggcat aaccgtttac gatcaatgca actgcatctt cctgtgaaat tcctctctga
    29401 ttgcaataga acattatatc ttcaccaatt tttgaagtag ttgcttcatg ctccacatga
    29461 gcacttttat tttttatttc aaagtaagga aaagtatgag ctccgcatct gtctcccatc
    29521 agcagtgaat cacattgtga aaaatttctt gcattgtcag cacgcttgct gacctgtacc
    29581 agtcctctgt aactgttctg acttttacca gcggaaattc cttttgacac tatacgactc
    29641 tttgtatttt ttccaatgtg catcatctta gttccggtgt ctgcctgctg ataattattt
    29701 gtcaatgcta cggaataaaa ttcaccaatg gaattatcgc cttttaaaat tacgctcgga
    29761 tatttccatg tgatagatga accggtctca acctgtgtcc atgaaatctt cgaattttca
    29821 cctgcacaga ttcccctctt tgtcacaaaa ttatatatac cgccaacacc ttctttatta
    29881 cccggatacc agttttgtac tgttgaatat tttatttctg catccttcat ggctacgagt
    29941 tcgacgactg ctgcatgcag ctgattctca tcacgcattg gagcagtaca gccttcgaga
    30001 taacttacat aacttccttc atctgcaata atcaaagttc tttcgaactg accggtattc
    30061 tctgcattaa ttctgaaata ggtcgacagc tccatcggac atcttacacc ttttggaata
    30121 taaacaaaag agccgtctgt aaagacagca gaatttagtg cagcataaaa gttatcagtc
    30181 tgcggcacga cggatgctaa atattttttt acaagttcgg aatgattttc aatggcttca
    30241 ctcattgaac agaaaataat tccaagttct ccgagctttt ctttatatgt ggtaataacg
    30301 gaaacgctgt ccattactgc atccacagct acacctgcaa ggattagctg ttcctcgaga
    30361 ggaatgccaa gtttatcata agtatttttt atttcgggat cgagctcatc cagactttgt
    30421 aatttcttct ttgtattcgg tgctgcataa taaattatgt cattataatc gattggcggg
    30481 tatttcacat tcggccattt tggttctttc atttcctgcc atagtttata tgctttgagt
    30541 ctccactcga gcatgaattc aggttcattt tttttattgg aaataaattt tataatatct
    30601 tcattaagac cttttggagc tttatccatt tcaagatctg tataaaatcc atatttataa
    30661 tcactcgaag ttactttttc gagaatttct gcttcatttg acatattaat tggttgtttc
    30721 gtgagatttt ttaattagga ctaaattagt cttatatatt caaaaaataa aggcagtttt
    30781 aaagtaaatt cgctgaaaat tccaaattcg tacaaaacgt tgtttctatg aaaaaatagt
    30841 ttccttattt tcaaaaatac agtttattat tatgaatata aaacttaccc gcaatttaat
    30901 tttatttaaa acttatctta gaattccccg cctgtgataa attgctgggt tagagattga
    30961 aacggagttt catgttcagg tgcttatcca aactgaagtt tggacacgag cggtttccct
    31021 tttttgcatt tataaaatat tttgaacacc aatgccagtt attataaaaa gaaatttaaa
    31081 attaggatga ataatttgaa tgattttaat attttgatta ctcaatctga aatagattta
    31141 ttagtcatat ctgaaatatc agttttaata aaaaagagta agaattttta aaaactaaaa
    31201 catataaaat ggaaaataaa aagatcatta cagtcttcgg tgcaacaggt gcacagggag
    31261 gcggtctggt aagagcaata ttaagcgata agaattctga gtttacagca agagctgtaa
    31321 ccagggataa aaattccgat aaggcaaaag cgcttgcgga tcttggtgca gaagttgtgt
    31381 ccgctgatat tgatgatgca gagagcatta agaaagcatt ggaaggagca tacggtgcat
    31441 tctttgttac atttttctgg gatcattttt cagcagaaaa agaatatcag caggtaaccg
    31501 attttgtaaa tgctgcaaaa gattcggatc tgaaacatat aatctggtca acactcgaag
    31561 atactagaat acttgttcca ctggaggacg acagaatgcc gacgcttcag gggaaataca
    31621 aagttccgca cttcgacggt aaaggtgcag ccgataaact ctttactgaa gccggattgc
    31681 cggttacttt tttaaggact tcattttact gggacaattt aatttatttt ggaatgggtc
    31741 ctaaaaaagg tgaagacgga aattattata ttgcattccc aatggatgat aaaaaacttc
    31801 cgggcatggc agcagaagat attggcagat gcgcttacgg aatattcaaa aaagacggag
    31861 aagtgacagg taaaactatc ggtgtcacgg gtgaaaaatt aagcggtgct gagatggcgg
    31921 aaaaactttc caaatcactt ggtataaatg taatttttaa taatgtaact ccggaacagt
    31981 ttcgaaattt cggatttccg ggagcagacg atttgggaaa catgttccag ttcaagaggg
    32041 atttcaatga ggaatttaat ggtatccgag atgaagtata ttcaaaagag ctgaatcctg
    32101 atctgatgga ttttgataaa tggctttcag aatatggcag taagattcct ttagaataat
    32161 tgttcatagt atcaaaatac attgtaatac tttttgggat atttctgatc acagcaggta
    32221 tattgatgtt tattgcacca aagaaagtgc agcagatcat tggaaaagcg ggcagtacaa
    32281 attttataaa ttatgcagag atcacaatcc ggatgatacc tgctgctgca cttgttattt
    32341 attctgatct ttcaagatat ccggatttgt ttaaagtctt tgggtggttt atgatcatta
    32401 cttcatttgt tttgtatctt attccaccta aaatgcatca tgctttttca gtaaaaagtt
    32461 ctgggaaatt aaagccgtta tatcttcgta tcatttcgcc attttcgata ttgataggta
    32521 tttttcttat ctattgtgtg tccatttaaa atttataatc ttaaatatat tattataaaa
    32581 aaggtcatca catttatgca accatgagaa atgtcagtgg taaaaatgca actccagcag
    32641 cagagttaaa agagtttgca aagtcaaaag aacttaaaat tgatgcaaca gcagccagta
    32701 ttgaggtatt ggctgcttct cttatggttt tatttttcag ttttaaattc atctacctga
    32761 ctcagtaagt ccattgagtt taacaatggt gcccattcct ttccattaaa aataatccgg
    32821 aaccattttt tgtttcgatt ttcatatttg atattaaaga aaagccagag agctgcaatg
    32881 gtaaaagaaa gtgtaacaat tccctgaatg atcaataatg gcaaactccc attttccagc
    32941 atatttttat tccaataaaa tatagtccac aaaggaagct gaagaaacat aaatcttgtt
    33001 acccaaagcg aagttgatat taactttgca attttttcct gtgtagccag aattggttct
    33061 gtaatgtctg tttgataaat ggtgatcgtc tgataaagat aaatgaccaa tgcaattgct
    33121 gttaataaaa tctgaatagc cgccgaataa agaaaaaaat gattgacact tgagtaagcg
    33181 gaaagaaata tatttgttac aactgtcccg ccgattccca cccataaaac agccatcata
    33241 acggtaaata ttttaactgg cttcattgaa gaaagaagcg attgtgtttt cattttgatt
    33301 aaatcttctg catttttttt attaagcaaa aggttttcat ttattttttt actgttggac
    33361 ttccaaagat taatgatttc tatgtcattc attttaatta tttattgatt tataatttgt
    33421 gaaaattttt ctttaagttt taattttatt ctgccaattt ttgtagctac attggtttct
    33481 gaaaagccaa tgatgtcagc aatttctttg tagctttttt cttcaaggta aagcagtatc
    33541 agagctttgt ctaactcttt aagttcagaa ataaattttt gaagaatacc cagatttata
    33601 ttttcctctt ctttttcaat attgatcgtg tctgcaatat taaagatttc agtattgaag
    33661 ggactggata ttttcgacct gctattttct tttctgtaaa atgaaatagc aacgttcagg
    33721 gatatccggt aaatccatgt agaatattta tagtcgtcat tatagctgtc aaacgattcc
    33781 cacaactgaa tataaatctc ctgtatcaaa tctttgcggt cttcactgtc tttacaatat
    33841 gcatttgcaa ttttgtaaag aataccttta tgagattcta taactgaaac gaatatggcg
    33901 tttttatcct gagtactctt cactatttat tgttgtaaat gttttgaaac cagttttgaa
    33961 tttctttcat agctttttta atttgtataa aattattaag aattactgtt tcttaattat
    34021 tcgcatttga ttcaaaaaaa tcacacttgt tagaatgttt ttttgaaagt agttttgaca
    34081 tttacaagat atgttagttt ttgatgctaa gctatgacta cacctaacta tttttttacc
    34141 ggcaatttac ttttaaattt taaatatatg gctgaaataa aattagttat tcaggattga
    34201 taaggtatag atatttataa aaatgatttg acaaatgccg gatatcaggg aacaacaaat
    34261 agaatcatat cccggataaa tcttctgacc aaaatattac agtgatgaaa ctgcgtgagt
    34321 cttgaataga gatttcattt gaagtatttt tacaaaagca cgtttgaacc taaatatcga
    34381 aatcaataaa ttggaattta aaccaacagg atccgtaaca tttctattca ccgacattga
    34441 aggaagcact aaactctcgc aggaatttcc tgattctctt caggcttcac tggatattca
    34501 tcacagaatt ttacatgaag caattgaatc caacaatgga tttgtatttg aaataatcgg
    34561 cgatgctttt tgtgcagctt ttgaaaatgc cggtgatgca gtgaaagctg cagtcgatgc
    34621 acaattatat cttgcatatg agaagtggtt taatgccgtg atcaaagcga gaatgggaat
    34681 tcatagcggc atcgctgaat ggaacgggaa aagatatttg ggttacatta cactggcaag
    34741 gacagcaaga gttatgtcag cagcatatgg agaacagatc attgtttcaa atgatacttt
    34801 tgaatcactc tttaataatt gccacaaaga ctcgaagact ctaatatttt tggatcctaa
    34861 aacttcgagt cttagagact ttgaggtaga agaaagaaag gaaatttcat tcagagacct
    34921 gggtgaaaga agattgaaag atgttattaa acctataaaa ctttttcagg tcactgctcc
    34981 cggcctcaga gaagattttc ctccattgaa aactcttgat gcacgtctga ataatctccc
    35041 tgttcagctt acaaatttta tcggcagaga aaatgaatta aagcaaataa aagattcttt
    35101 gtcaaacaca catttattaa caattaccgg atcgggtgga tccgggaaat caagattgtc
    35161 attacagaca gcagctgatc tgatagacat atttgcaaac ggagtctggt ttgtagagct
    35221 tgcaaattta aatgatcctg tattattacc tgtgtcaatt atgtcggtaa ttggaatttt
    35281 agaaaatccg aaagaatctc cggaagtgac tttaaaggga tatttaaaag acaaagaaat
    35341 tctgatcatt cttgataact gtgaacacct cataaatgcc tgtgcagttt tagtagaaag
    35401 tttactttcg ttaagtccaa agttaaaagt tttagctaca agcagagaac ctttgaaatg
    35461 ttccggtgaa catttattca ggatcccttc attatccact cctgtccaag gatcaaaaga
    35521 aactcctgaa cagctgacac aatatgaatc tgtccggttg tttattgaaa gatcattgac
    35581 gttagaccag ggtttcaaag ttacaaatga aaatgcgtct gcacttgctg agatctgttt
    35641 ccggttagat ggaattccac ttgcgattga attggctgca gcaaggacaa agatattgtc
    35701 gttagaaaaa atatttgaga gattagatga cagattcagt ctgcttaccg gaggaaagag
    35761 aacagcattg ccgagacagc agactttaag atcactgata gactggagtt atgatcttct
    35821 ttctgataat gaaaagataa tctggaacag attatcagta ttcagaggag gttgggaatt
    35881 ggaggctgcc gaagaaatat gttcagatga tataatagat aaaaatgaaa ttatagaatt
    35941 actgcaaaat ctttcagaga aatccattat tatttttgat aaagaacttg tgagataccg
    36001 aatgcttgag actatcagac agtatggaga tgaaaaatta agaacctctg aagattataa
    36061 ggagatatct aaaaagcatt taaaatatta tactgaacat gcagaaaccg cagatttgaa
    36121 attacatggt ctggaaattg taacatggtt aaaagtttta gatgtagaaa aaggtaatat
    36181 agaaaaagta tttatgtttt ccgatgaatc aggggatata attacagaag caagattagc
    36241 cggcgcattg ggatattact gggatataag aggacaaatt tcagaaggaa ttctacgcct
    36301 ggaaaatata tatttaaaca ttccggaaac aaagaatctg ggtttcatta aaattatttt
    36361 tctgattgga ttacttaccc atagaaaagg tgaatttgta aaagcatctg aatatcttaa
    36421 agaaagtcat gatcattata taaagctgaa ttacaaaccg ggtatcggct atgcattaaa
    36481 tagtcttggt gatattttta gggataaggg ggaatatgat aaagctgctg acatgtataa
    36541 agaaagtctt gaaattcgcc ggaatatcgg tgacaaaagt ggaattgctg attctttaaa
    36601 ttgtctggga aatgtctcat ttgataaagg agaatataac gaagctgatg aattgtataa
    36661 aaagagtctt gaaattcgcc ggaatattgg agacaaaagg ggaactgcta attctctcca
    36721 ttggcttgga aatgtatcaa attataaaag agaatatgaa aaagcaacgg gatattatag
    36781 tgagagcctt tctatacgac gtgaaattgg tgacaaaaga ggaattgcca gttctcttaa
    36841 taatctggga aatgtttata ttataaaaaa agaatatgaa aatgcaactg atctatatga
    36901 agaaagtctg gtaacattcc gtgaaattgg agataaaaaa ggaatagctg attctcttaa
    36961 cggacttgga aatgtatctt ttgataatat tgaatatcaa aaagctgcag atttatatca
    37021 agaaagtctt gcaatcaaac ttttaatggg agaaaaaaaa ggaatagctt attctctcaa
    37081 taatctgggt aatgtatttt atcataaagg agaatatgat aaagcagccg gctattatga
    37141 ggagagtctt gggatcaagc gtgaaatcgg tgataaaaga ggaataacaa attctcttaa
    37201 cgggcttggc aatgtttttt tttataaagg agaatatgat aaagcaacca gctattatga
    37261 agagagtctg gaaatcagtc gtgagataga ttatgaaatg gcaattgagg attctctaaa
    37321 aaatcttggg atggtttcta taaaaagagg tgaatttaat aatgcacaga aatattatca
    37381 ggaaattctg tctcatagta ttgattcagg taataaaaaa aaaatcacag aaaatttatt
    37441 gagacttatt gatctgaatc tgaaaaaaaa atcagatgtt tcattcaata aattattagg
    37501 ctttttaaat cattattcaa attcaaacaa aatcatcttt gaaaaatcgg atcaaaaagt
    37561 atttgatgat gcggtgacat atctgaaaga gaatatgagg gaagaagagt ttttgaaaag
    37621 ttttgaggaa ggggaaatga tgacacttga ttcaatttta aatattaaat tgtaaacact
    37681 ttattaacag atgaaaaatt taaacagtat aagtattttt gaattaattc ttaattttta
    37741 atttttaatt tgactatacc taccgggaat ataacatttc ttttcaccga cattgagggc
    37801 agcactaaat tatctcagaa atttcctgat acaatgcctg ctgcaatgga aattcacaat
    37861 aatattttgc ttaaatccat agaatcgaat aatggatttg tatttaaaaa tacaggcgac
    37921 ggattctgtg cagcttttca aaatgccggg gattctgtca gagcagcggt tgaaattcaa
    37981 acaaatcttt taaatgaaaa ctggaaagat gctgtcatta aaatcagaat tggcattcac
    38041 agcggcaaag ctgaatggaa tggtaagaac tatatgggtt atattacact tgcaagaact
    38101 tcaagaataa tgtcttctgc tttcggagat cagatcatta tttcaaatga tacttataat
    38161 ctgttttctg agagtaatac tttactacaa agtcacgaaa atatcagatc tgaaaaaaac
    38221 aacttgattt ctttgcatca aagtggtgat actaaaattt cattttggga tcttggtgag
    38281 agaagattaa aagatctgat caatccaatc aaactttttc agattacagc tcccggactg
    38341 agggagaatt ttcctccatt agaaactctt gatgccagac caaataatct tcctgtacag
    38401 ttgacaagtt ttatcggcag agaaaaagac attaaagaaa taaaaaagtt aactgtcgat
    38461 aaccgtttag tatcaataat cggacccggt ggaataggta aaaccagact ctctttacag
    38521 gtagcggcgg aactgataga tcaatattct gacggagtat ggttaactga gtttgcgcag
    38581 atttctgatc ctgaattaat ttcacagaca acagcaaaat tcttaggtat taatgaacag
    38641 tcaaatcagg aaacaattat tactcttatc aattatttaa aggataagga attactttta
    38701 atatttgata actgtgaaca tttagtatct gcctgtgcag tacttactga aaagctcttg
    38761 caaaattgtg caaaacttaa gatcattgtt accagcagag aagcgttcag aatcgaaggt
    38821 gaacatttgt ataatataat ttctttgtcg catcctgaac caaatgaagt aaatactcca
    38881 attgaattat ctcaatttga agcagtaaga ttgtttattg aaagagcttt atcagtcagt
    38941 cccggattta aaattgataa tgaaaacgca cctgcactgg cacagatctg ttatcatctt
    39001 gacggaatac cttttgcgat cgagcttgcg gcagcaagaa ctaaaatttt aacgcttgaa
    39061 aaaatatgtg aaaaattaaa taacagattc cagctgttaa ccggaggaaa acgaacttct
    39121 cttccaagac aacagacttt aagagctatg atagactgga gttatgatct gctttctcat
    39181 aaagaaaagt tactgcttca gcggttatct gtattttccg gaggttggac aattgagact
    39241 gcagagcaaa tttgcgctga tgaagaaatt gatgattcag agatactcga tttaaattca
    39301 cttctcttag ataaatcttt gataagtgcc aaagaaagtt caggatctat caggtttttc
    39361 atgctggaaa caattaaaga atatacgaag gaaaagcttg aaaaaatgaa tgatgtttac
    39421 cggaaacatt tttcttattt taatcaattg tgcaattgtg atgaaatgag atcaaaaggg
    39481 ctgacacagc ttcaatggat aaaattagtg gatactgaac tggataatat cagagcagct
    39541 attcaatggg ctgtcgaaaa aaattctgat gaagcttgtg atattatcag tactactggt
    39601 atatttaata atatcaaagg ctattataaa gaagggcttc agacatgtct gaaaatttta
    39661 aattcatcat tatcggtaaa tgaattgcca aaagtaaaag ttctgtatac agcggcatta
    39721 atggcttcta atcttggaaa ttctgctgat gcagaatatt atgcaaagga gggtcttaga
    39781 gttgcaaaaa aatataacga taaacataaa attgcaagtt gtctcaatgt tctcggatct
    39841 tctattaata aaaatctgga aagatcagct gaagcacttg aatattttga ggaagctctt
    39901 tctatcttca gggaagtgaa taataaaaca gaaataggtg tattactttt taatatgtca
    39961 acaacggtaa atataaaagg tgatccaaaa cttgtgttga aatataggct tgaagcaatg
    40021 aaaatattta aagaattaga tgatactcat aatatatctt taattcttgc aagtcttgga
    40081 gtatatgaat taagggaaaa taattttgaa ctcgccaaaa actatacaga agaaagtctg
    40141 gctattgctc atgagatcgg tgataatttt ttaatatctg ttaatttagt caatctgggt
    40201 tgtatttatt caggattaaa tcaatatgaa aaggcagctg aattgcttga aagatcaatt
    40261 gtaattataa aagagcatgg ttataaggaa agttatcttg tagctgtgat gtatctcgga
    40321 gaagtttttt cgaattcagg cgattataaa agagctattg aacttttcaa tgaatccatt
    40381 caatcaggaa cagattcaaa taatgattat tttcttgctt ataattattt caatttaggt
    40441 gtttcgtatt ttaaattaaa agactatgtg aattctttga agtactttta tctattaaaa
    40501 tccgacaagg aaaattctaa acttaatata agtaatgaga aattgaaaat tgctgaggag
    40561 tatatcagta aattagaaag tattgaataa aggcaatgtt taatgtttaa tgtttaatgt
    40621 tcgattttcg attttcgatt ttcgattttc gattttcaat tttcgatttt atatatttaa
    40681 ttatggaatt taagccaaat gtaaaaataa tataagatag tatggaaaat aagctgcatt
    40741 taacagagct ggctgaaaaa gctgaacagg aacttgaagg ttctgaaagt cagagcaaag
    40801 tgtttttgaa gaatctatat taaagcttaa aaagaattta ggagataaag agtttttaga
    40861 gagatttgaa tcagggaaat cgatgacgct ggatggaaca gtagacctag ttttaaatat
    40921 taatttatca aaggaataga ttaattttaa aatcaatttt ggcataattt ttaattatta
    40981 ttttgaataa tccaaccgga caagtaacct ttttatttac cgacatcgag gaaagtactt
    41041 tttcaatttt aaattttaaa ttttaaatgc tcaattttga ataataaacc aaacggatca
    41101 gtaacatttc tcttcactga catagaggga agtaccaagc tttcacagga atttcccggt
    41161 acactgcctg ctgcacttga agttcacaat aaaatattac gggatgctat agaaaccaat
    41221 aacggatttg tttttaagaa tacgggtgat ggattttgtg ccgcttttga agatgcattt
    41281 gctgctgtga aagctgctgt agatgcacag tcaggacttg caaatgagaa atggtctgat
    41341 gctgtgataa aagcgagaat gggaattgac agtgggaatg ccgaatggaa cgggaaaaat
    41401 tatattggat acaaatcact ttcgagggca gaaaaagtta tgtcaactgc ttatggagaa
    41461 cagatcatta tctcgggaga ttcttatgaa ctggtaaaaa agaattttga gagttctgat
    41521 aagaagatct ttaataaggc tggaaatgaa attacattta ccgatcttgg tgaaagaaga
    41581 ttgaaggatg agaacaatac ggtaaaatta tttcagatag caacttcagg actgaaagaa
    41641 gattttccat tattaaaaac acttgatgcc agaccaaata atctgcctgt tcagcttacc
    41701 agttttatcg ggaaagaaaa agaagttaaa attataaaaa aattaatagc tgaaaaccga
    41761 ttagtgacat tattcggacc tggaggaaca ggtaagacca gactttcttt gcaggttgca
    41821 tacggactga tagaccattt tacaaacggt gtatggctga ttgaacttgc accgatttca
    41881 gatcctgacc tgatagctca gacaattgca aactctctga agatcaatga tcagtctgac
    41941 caaaatacaa ttgaaactct taataattat ctgaaggata aagaactgct tttgatcata
    42001 gacaactgtg aacatttagt tactgcttgc gctgcgttta cagaaaaatt attgcagaac
    42061 agtaagaatc ttaagatcat tgccactagc agagaagcat tcagaagcaa aggggaacat
    42121 acttattatg taacatcttt atcgcatccc gaactaaatg aagtagtttc actaaatgaa
    42181 ttgtcacaat ttgaagctgt taaattattc atatcaagag ctttggaagt caaccccgga
    42241 tttagtatta acaatgaaaa tgcccctgca ctggcacaga tatgttatca gcttggcgga
    42301 attccatttg cgattgaact tgctgctgca aggacgaagg ttttaccggt agaaaagatc
    42361 tgtgaaaaat tagttgacag attcaaattg ctgacaggag gcaggcgtac tgatcttccg
    42421 ggacatcaga ctttaaaggc tatgatagac tggagttatg atttgctttc agaaaaagaa
    42481 aaaatcctgc ttcagcgcat atcggtattt tcaggcggct ggacattaga agcagcagaa
    42541 aagatctgtg aagatgatga aattgaagat cttgagatac tggatctgaa ttcacatctt
    42601 cttgataaat ctctgatcag tgcaaaagaa tcatcaggag agataaggtt ttttatgctg
    42661 gaatcaatta aagagtatgc acgggaaaaa cttggagaaa aaaatgaagt tttcaaaaga
    42721 cattttgatt tctttaagga gctgtctgtt tatgaaactt tcagatccaa aggagttaca
    42781 cagcttcaat ggatcaaact tattgataat gaaattgata atttaagagc tgctataaaa
    42841 tgggctactt taaattatcc tgatgaagca tgtaatatta ttagttcatc aggtgaatat
    42901 tataaaatca aaggctatta tctcgaagga catcagaatt gtataaaact aataaattct
    42961 tctcttccaa ttactgataa atcaaaggtt aaactttttt acactgccgg attaatgaca
    43021 gagagtcttg gaaatgaaac tgatgctgaa agctatggaa aagaaggact tgccatagct
    43081 cgaaagtata acaataaatt agaaattgta atgtgcctga atctacttgg agtcagcacc
    43141 aataagaatc tggcaagaag agaagaagct atgaaatact ttcaagaagc tcttactctc
    43201 gccagagaaa tagatgccaa aggtgagatc ggatcgatac ttcttaatat gagtcctata
    43261 ttaaacaaca aagaaaatca ggttatgaat aataaaaaag aagcattgaa aatttttaaa
    43321 gaacttggag atacccgaaa tgtatctttg atccttgcca gtattggagt atatgaattc
    43381 aaacaagaaa atcttgaatc tgctttagtt tacacggaag aaagtcttga cattgcaaat
    43441 ctgataggag ataaattttt gatctcagtt aatttagtca atctgggttg tattaattta
    43501 gggctgaagc agtatgataa agcagccgaa ttacttgaac aatcaattgt gataatcaaa
    43561 gataacgggt ataaggcaag ctatcttgca gctgtgatgt atcttggtga agtattatca
    43621 aagtccggtg atgatgaaag agcaattgga ttatttaaag aatcaataca gaccggaact
    43681 gatatggaaa ataattacta tctttcaaat aattatttca atttaggtgt ttcttatttt
    43741 aatcttaaag actatgaaaa ttctttaaaa cactttgatc ttttaaagat atgcttagat
    43801 aaaactaact ctgtacatag taatgaaaaa gttaagattg ctgaagagta tattaataaa
    43861 attaaaaata tgatgtgagg attatacttt gattaaattt ggatccggct ctacaagcta
    43921 attgaatgac tcttgagcat gaagtgattt aaattataaa tcaaaaattt taattattca
    43981 attattatgt ttttcattaa aatcaaaagt taacttctaa aaaattaaac caaatcatga
    44041 aaaaaattta cagtttaaaa tttcttattg tttttataat gttctattca tgcggcaatt
    44101 cttctgaaga tgtgaaagtt aattctgatg tgaagggcaa atctgaagtg aaaggcgaaa
    44161 ttgaattgaa ggataaatct gaagtgaaag acttacttgg aataaaaggt ccgctcgaat
    44221 ttaattcaac gacatttgat cttgcatggt ctgataagcc acgtgagaat tattacattc
    44281 aggaatatct gccttctggt gagtcgccag aaagttttaa tcagatgatg tcggtacatc
    44341 tttttttaac tgatctgaca gtagataaaa tggtgagtaa aaaaattggt gaactcgaag
    44401 acagaaaaaa gagtgacccg ctttgcaatt attcttcttt caaaagtcct gacggaaatg
    44461 aaattattgt tgattttgta ctcagtgagg ataaagaagg gcagatacca attatagaat
    44521 tcaatgctta ccgctataaa atatcagaca ctggaaatgg aagtaaagga ataattattt
    44581 atgcatattc gaaaagaagt tatggagatg atatagattc ttttcttgta aatttaaaag
    44641 aagaccgaat gaaatatctt gatgaaatgt cgaagacaac tattcctgca gtgtcaattt
    44701 ctaaatagag aattcgtaaa caaatattca ggtttgatca aactctttac agatactctg
    44761 taaataatca aacactttcc agttcctaac ctgggaagaa tgaattaatt cttaatttat
    44821 ttcagttccc tcagagtccg aaaagcacgt gactctgagg gaataaagta tattaaaaac
    44881 attcagataa ataagcagtg tattttaaaa gttagaattt tagtaaatta gcataaacaa
    44941 atagaatatt attaaaatga ataaattttt attaatactt gcagttttga gtatatatct
    45001 aaatacttcc tttggacagc ttccaaatca taatatcacc cttctggcaa atcttaatca
    45061 gcatattaca aacgatacct attcaggatg ctggggatat attgctcccg acggaagtga
    45121 atatgggata ctcgggactc atctgggcac agcttttata aatatttctg attcatcgaa
    45181 tattcatgag gttgactttg aacctgcacc ggtaactgga tcaggaaatc actggagaga
    45241 gatcacagtt cattctcatt atgcttatgt tatcactgag cagtcaaaca gcggaataga
    45301 aatttttgat ctgcagtatc tgccggattc agttcatcgg gtaaataaat ttattcctcc
    45361 gggtcacata acttctcata ctattgcaag atcaggtccg tatttatttt tgaatggttg
    45421 taattctgca ttcggtcagg gagtcacaat tttagatctt acttcagatc ctgaaaatcc
    45481 agttgtaaga ggatcattaa acacacatta tgtacacgac tgcagagttg taaatgatac
    45541 tatttgggct gcatgtctaa gcagcggtat cagaataatt aatgccgtta acaaagatca
    45601 gccggtattc ataaattcat ttctgactcc gccgcatctt gctacacata acagcgcatt
    45661 gacaaaggac ggaaattatc tttatactac tgatgaaacg agctctccta ctgccggtac
    45721 tttgaaaatt tttgatgtac aggatcttgg gaatattaca tttatcagag actggagacc
    45781 gaccggaatt acaacttcac aggtacataa tatagaaata ttcggagaca ctgctgtcat
    45841 cgcacacagg tcatcgggta tcagagtact ggatatttca aatccaattg atcctataga
    45901 gattgcatgg tatgatacat atccaataaa taacgttaac gcttttattg gtgcttgggg
    45961 aatatttatg ttcccgtcaa aaaaaataat tgcatcaaat aaagaagatg gattatgggt
    46021 agttcagctt cagaatacaa cagacattaa attgaattca actgttgtgt cggagtattc
    46081 actttcgcaa aattttccta atccgtttaa tccgaagact gtaatcagtt acgaattacc
    46141 agtgacgggt aatgctttgc ttaaagtttt tgatgtactg gggaatgaag tcgctgagtt
    46201 ggtaaatgaa aagcagaatg cgggaagtta ttcagttgag ttcgatggaa gtaatttttc
    46261 gagtggggtt tatttttata agctgacagc tggggaattt gttgagacta agcggatgat
    46321 attattaaaa taaatttaaa attatttatt aacaacttcc gattttggac tgcggtgttc
    46381 gtaataatta attatttttt ttcagaattt catcatatac tttttttgaa tgttccacag
    46441 aatatttaaa tgtaccgcga tgatcagcaa gtttaccggc agagacagag atcgtttttc
    46501 cgcctcccat taagagctga taatcaacag gaagtgtacc tatataaaca ggaacagctt
    46561 catccgactc tccgaaatat agatttaatg gtgtcctact tctccatcgg tacgaattat
    46621 tgttttgaat aatttgccag tacctgtttt ctcctgaaga gcttgactcc ttaaattctg
    46681 atttcagata atcgtcaaca ttgtcaggtg tctttgaata aaattcttca aatgtaattt
    46741 tattctcata aaaatccctc gtcgcctgct gatattccgc ttttatgcct gattccatta
    46801 acccgggcag atcataatag atagagtaag agcttaactg taaagctaaa atacccggca
    46861 gccagacagc atcacctttt tcaggtgcat gtatccagcg gtttatcatt gcataaacat
    46921 ctgtcggagt gcatgcaaca gaagttgatg ttacttcaat attcaggttt tcaagtttct
    46981 gcaaaaaaag taaagttgcc cagcttccca tcgaccaacc gctgagaaat aaaggaccgt
    47041 gctcaatgtt catcgaattc agaatctcat ccgaagcttt gagcatatca agacatgcct
    47101 gttgtgtact tgcttttacc tgatagctgt cctgttcaga agataatcct ttaccaaaat
    47161 aatcagcacc gatgactata tatccattac cagcaaactg tgctatcata agtctggtct
    47221 ccattgattc atctggaaaa gatggcacgt cggtttttgt aaatacagtg ccatgctgat
    47281 atgaaacgag aggcatcttt ttattttcaa tatcaggaat tgcaataagt cctgatgcaa
    47341 cagtgggttt attattcatc tcaggaattt cagaattata gatcactctg taaagcttta
    47401 caccatttgc aacagaagaa aaagtaactt caaagtcaga gaaagattca gcttcggttg
    47461 taaaaatttc gttcagtttg ttaacatcat aactgccgat atatttataa cctacttcag
    47521 aattaacata agtaaaatct tcatacgttt gtgagtagag atttataaaa ggaataaata
    47581 agatcagcag taatgtaaat aaattttttc tcatttttta ttgtttgctg cgtttcagat
    47641 aaataacctg agattaattt ttattaaaaa agtttttatc acattaaaaa gaaaatttga
    47701 aatatcaatc ggaggttttt tgaagatatt attttaaaag acttttgttc cccgaattat
    47761 taattacata aaattcacca ttctcaaaaa aaagcgcgta cataaatcct ttatctgaat
    47821 tttcctgatg atgaagatct atcccgatca ctttcttatt atataaatat tttatgctgt
    47881 ccactactgt atgaccaagt accattttat tggcattaaa aatttccaat gtactgtcaa
    47941 cttccgcctg agtttcttct tcttccataa ttcctctgta ccatattgga cttttgctgc
    48001 cgatgaaaat atttttcctt ctctcttctc tttcaaaatc tatgtctata ctgtatctga
    48061 tactgtcatt tatttgagtc aaagtatatt taccataagg aacatcttta ctcaatcctg
    48121 catgcacaaa caagtatccg cctatatttt caattgcgtt tttgcttcga agccatctgc
    48181 cgagttcagt ttccggtgaa taccatttgc tgtaatccag accaagtgaa tcagcattgt
    48241 caaagtattt ttgtctgaca tatctcaggt cattttgtaa taccattatc tcatgattac
    48301 caagaataaa atgaaccttg ccaccgtgca tttcagcttc actctctaat ttgtagataa
    48361 gccacagaca ttcatttaca tttattcccc tgtcaaaaaa atctccgttc aggacaagat
    48421 gtccattccc aaaagtccat tcgaaatttt catttatcac gccggctcct tcgagtatca
    48481 tctgaaaacc tctgaagttt ccttctatat ctgaaatgat gagcattttt tccggaagct
    48541 cgtaagtgtc tttctcaatc tgtatatttt cttttagttt aaaatcaaac tgattaactg
    48601 tctcatctac tatgcaggtc aaagtatcat ccttagaaat ttccccgaca gaaatttcaa
    48661 ataaatctcc atcagtttca ttcatatttg gaattactga ataattcaaa acttttgaat
    48721 cttgataaac tatgacaggt ccgtcactgc tgaatcctga gctgtccaca tcttcatctt
    48781 ccggcatttt taattctttt ccatttagaa cagcaggtgt aattaaataa actattattg
    48841 ttaatgaaat tagtattact gtaagacttg acttcaaaat taccataaaa ttatttaaaa
    48901 tacatttaca ttgatacctc ttctttgtca atcttttctc ctctgaaaac tttgaaaaat
    48961 cccagcgcaa ttgccagaat tatcggtccc atgaataatc ccaacaaccc gaataactga
    49021 attccgccga gcacactaaa gaaaacaaaa agttcattca tttttactct ttgctttata
    49081 agtttaggaa gaaggaaatt atctatcatt ccgataacaa gtgcagcata aacagaaatg
    49141 acagctgctt ttgcatattc tcctgaaaaa agaagaacga taattacagg cacggtaaca
    49201 aaggcagtac ctcctgttgg tattacagca aatatcatcg caagtacgcc aagcagaatg
    49261 tatgatggga tgcctaatat ccagaagatc aaacctgcta aaactccctg aagtacggca
    49321 ataaaaagag taccgcgtat tgttgcattg ataagtgctg atgtttcacg gataagttct
    49381 ttagactgac tgctttccat tggcaaaatt tcaggaatat tcttcacgat cttttcacca
    49441 tctctgaata aataaaacat gctgaagaca gataaagtaa ttccgacaat cattccgaaa
    49501 gcaccttcga taaaatacaa agaggcagac aagaatgcct ctttaatatt atttataaaa
    49561 cctttaatat cttcgggttt aagaaattct tcaattcgaa taaattgatt caaataatta
    49621 tatataaatg aaaatttacc tttttgaaga tcggacaata ttagtttaat ttcgtctaat
    49681 gatgtccttg aaattccgga aatttcgttg attgcagaag ctaatactgc cacgacagga
    49741 attacgaaag taaataaaga tacaaatata gttatgatag cactaataga gtggttattt
    49801 gttttaagaa aaagtttttt atatacagga taaaaaataa tggcaagaat tgcagaccat
    49861 aacaaaattg atataaaggg ggcaagcatc agccagcaaa tatatagaag taaaccggta
    49921 atgaacatca tcaaaacaaa ttgagacatt tgttttttat tgttcagatt attttcagaa
    49981 ggcataagca attttatttg tgaattttga aatacaggga tcagaaaata tattagatta
    50041 actaatatat aatttaaata ttagactttt gtttaattat cacaaaaatc tgacaaattt
    50101 gagtaaaagg aaatctggct tattcacctt acatttctct tcttaatttc ataaaaaact
    50161 ttatcagctc atgttgatca ttttcatttt gtatcctgaa tttgaaaaaa gcctcaagat
    50221 tctttaaaat attttttctt ctttgaatgt ctaaaagtat taagaaatca tgtgtttttc
    50281 tgtgatcttt gaatcttgtt tctatgtaca tatgattttt attccttagc catttgttct
    50341 gaaatttatt ttttataata taactgtaca aatatttatt tacagtcaga gatcttttcc
    50401 ctgcatcttt tgcaacaaag gtttttataa aatttatata gctgatcctg aatccaaagt
    50461 aataatttcc catatagctc ttcctgatat atttattgaa atcattatcc tttgagaaaa
    50521 tgatcatcat atttctttta tcagtacctt tatgcagaaa gaatgaatct tccatcggta
    50581 tagttttaat gattccgtgt tttacctgat atggatcaga ttttgccgct aactttatca
    50641 gaaatgaaat attatatccg aataactttt cttcctcttt gtctaaataa tatcgaaaca
    50701 ttatttgaaa ttgattttca tattatactt tttatgtagt gaaaatagat ttaatagttg
    50761 agaaattaaa agaactaatt catgcttttc aaaatttaac aacatatctg tatctgcgaa
    50821 tcctcttata attccgcgaa actattcata tattttttaa taaatatagg tttttgtttt
    50881 gacctgtatt gcatcacaaa tctcggatgc tcgagaggaa ttactttccg aaaaaaatta
    50941 tgttgatcat ttatgagttt cataaattta aaatttttac ccgtccccaa acaaaaacaa
    51001 atatccgtct cgagaccaag atcaatttgt ttctcaagtg aatttaaaat gaatggataa
    51061 acggattttg ttaatgccgg atcatcataa taattataat ttacaaattt acctttttca
    51121 tttcttatag taaatccaag cggacagatc gagttaatat aaaatttgga ataaaatttt
    51181 tccggaccgc caaacgtatc aataacttca taaacaaata cagaagatgg ctcgtatgtt
    51241 tcgcttccat taaacctgat tccacagtta tctttcagcc ttttagtatc ggtaaatggt
    51301 atgcccgtca ctcctgcacc atttcgcccg ggattgatcc ctgctacaaa aaatcttttg
    51361 caattgtcat tgtaaaattt cttataaaac tgagtcgaag actcaactgc agacttctct
    51421 ttaaaaggat tcattgaatt cacaccttca ggaagtgcag atgagatctt taatctgctg
    51481 ttaaatgtaa ttactctgtc agcaaaattt gtcatgaaag tggtatgaag ttattataaa
    51541 attttattaa ttggtttttg gatttgcaat tattggtata atttctaata atcatattct
    51601 gttggaatga aatattctga atttaaaact cattcagaat cgattacaga tttaatttgg
    51661 ttcgcgatac ccagttggga acatcagctt cattctgacg aagtaaaaga ttcagctgtg
    51721 cggcatgatg ctgcacatgt ctcatattgt ataaaagtat ttcaggaatg ctgtaatccc
    51781 tgtattcatt tttccagcgg tgcaatacat tgtcaggatt catacctgcg atcaggtcgt
    51841 gacatttatt ccggcagtgc atcagatata tcagcatctc ttcttttgta taaacacgtt
    51901 caggcatttc tccttcagga ttaaattcag aaagtgtaaa tggcggagga ggtgaaaaat
    51961 tatccggatc tgtagtaaga tagtaatcaa gaaagaataa agtatgataa actatatacc
    52021 agtattttga atccgcatcc caaagatctt caggacatgc cctcactgca ttttcaaaca
    52081 tatctattga tgctccgaac tgtttccaca ttacttcttt taataatgtt tccataataa
    52141 atttaattgt taaaaaaatt tttgatttat tttgttgatc agatcagttt aggaattatg
    52201 cgtttaacat agtttccata tcaagttttc tcatctttaa tacttcctgc atgactctcc
    52261 ctgctttttc aggatcactc aataattcgg caatgttatc aggtacgatc tgccatgaca
    52321 ctccatattt atccttcagc catccgcaca tactttcttc gcctccatca gtcagcttat
    52381 cccagtagta atcaatttct ttctgatctt tacatgacac gacgattgat acagcttcat
    52441 tcatactaaa ttcatgtcct tcactttcca tcatcataaa tacttttcca ttgagactga
    52501 actgtgcatg tttcactaat ccttctttct gatcttctcc ttcagaatat ttcatgatcc
    52561 ctgtaattga agaatcttta aatacagatg tataaaaatg aattgcttca tctgcttttc
    52621 caagctgatc cccgctaaac agcattgaag gcgtaaactt ctgtccgaca tcttcatact
    52681 ttccaagcat taactgccag cttactccgt atttgtcctg gatccatccg tatctttcgc
    52741 tccagtcata tttatccagc ggcataaata cctttccatc atcggagagt ttttcccaat
    52801 gcagatcaat ttcttcgacg gattcacaaa gtataaaaaa cgaaatcgaa ggattgatct
    52861 taaatttcgg accgccgtca agtgtcataa atttttcacc gctcaattca aaagttacaa
    52921 ccggaccgaa ctctgcagta atttttgaat ctttaaataa attacaataa aattctgatg
    52981 cttcttttcc gagtccgtca aaccataggc agggataaat ttgatttttc atttaaagtt
    53041 tatattaatt tagtaattaa ttttttaaat agtagattta attttaattc agtatccgca
    53101 gagaacttta tagttagaaa acttagatat cctttttctc atacaccagt actgcaatac
    53161 cacattcata aggagtacag ttttttaagt taagcttctg tgttgaatca agtcctttga
    53221 aaattgacat gccacttcct atagctgcag gatttacgaa caaatgaaat tcgtcaatta
    53281 atctttcact tatcagcgaa gccacaaaac ctgcgccgcc atatactata atatctccgc
    53341 cgtcctgttt ttttaatgaa ttgatttcat cagaaagatt tccttttgcc agttcggtat
    53401 tctcccattc ggatttttcc aaagttttgg taaatactac tttcggttta tccatcatct
    53461 tatgtgcaaa ttcatatccg ggatcatctg gaagttttct gacattcgtc cagtgcgtaa
    53521 tgaaaccttc tgtcattttc cttccaagta aaattgtatc ggatgtgtcc gtgatcgaat
    53581 tgatgtactg cgaaagtttt tcatccatat cccaggtcat ccaatccaat tcaccattag
    53641 gacctgcagt gaatccatcg acagttatct gtgtctgaag ttttaatttt ctcatgaata
    53701 atattaaatt ttaaaaataa acttatacaa aatacttctt ttaatataaa tataataagg
    53761 tgtatatgtg acatattgaa ggttcaatta cgacaactta attatcataa aattaactca
    53821 aataaaattc gataaagtta atttatatct atccaaaaac ttaatacact tactccttca
    53881 acttctttct cctgataaaa tattccgtta tttttcaaga tagttttttt tgatgcaatg
    53941 ttatttttgt tacaggttac aagagctttt ttgatgccaa gttttttaga ctcttccaaa
    54001 gcaagttcaa gaatttttgt tgcataacct tttcgtctct gtgacggtct cacgccgtat
    54061 ccgatgtgac cgcctatcag actaagcttg tcattaagtt tgtgtcggat attcaccact
    54121 cctaaaattt tatcttcaga atttactaac cagaaagttg agtgaggaac aaatgtctca
    54181 ggtatatcta ttccgttttt aaatccttcc atctgttcta caaatttttt aaaattcttt
    54241 ggatcatatc tcaatataaa cggtacaagc tcttctccag aatcatgcca ttcttcaagc
    54301 atttctttgt attcgttttc cagattaatt gaaggagata taagtttaag agttttcaat
    54361 atttaaattt atattataat tcttttctga atcaacagtt actttataag attttttaat
    54421 cttcaatact ctgagcaatt ttcagaagtt cattaacagt cttccagttt cgggtagtcg
    54481 cagttacttt cagtttgttt tcaataaaac catttgtcag ctttgaattg ctgtacttgt
    54541 ccggacaata aagataaact gcattatcct tatgataaaa tttatctttt ttaaaatcaa
    54601 tttcagatag ctttttgaca tgattattat caggaatatc agataaaaaa gttacatgca
    54661 aatatgctgg atctatttct gtttcctttg agaatgggtt agatttgatt attcgctcca
    54721 catcttttaa ttctaatacg atcaccggta catcgaatcc aaatgtttca tttatactgt
    54781 ctgaaatttt ggcggataat ttattcggtt cagtattttt aaatttaaaa ataatatttc
    54841 cactttgtat ataagacttt acatttttaa attttaactg ttcaaacatt tcccgtaaaa
    54901 catccatttt aataatcctg tgtccgctta cgtttatacc tctcaatatt gaaatataag
    54961 tcgtcataat atatcaatga tatttttatt tatggaaata attaatggtc agtttaattt
    55021 ataaatgttt actatttttt ttctatataa aaattttttg agccggattt caaattgatt
    55081 aaaatgaatt caattacatt atgatttaat tagactccaa actgcgacaa atgatgatcc
    55141 agatgtttat aaaacatatt attccattct gttttgttaa ggtttccaaa ggatagattt
    55201 tctttattat caaaatgacc ctcacctagt tcctgagttt tatttataaa atcaattaac
    55261 cgtttctttt cagtatcaaa attttttgaa tcagtcataa taaactgtgg ggctgttcta
    55321 aggttatggc tgtaaggctt ttcaccaacg attttatctt ttacaataag ttttaaaatg
    55381 aaactcataa atgcatttgg ttttttatgt ttggtttcat acaccatttc atatacaaca
    55441 gtacagtgtg caagcatctg cgaagcattc atctttcccc aaagaggtgg agtttccgaa
    55501 cttaaattat ttatcctctg aattattttg tcagagactt cttttgtgaa tatgtttggt
    55561 aatggcatgt cagcttttta attttaatta aatttctttt ttgtgtgaat cgcaattttt
    55621 caaatttata gatttatatg ttaatatgaa atgtgtaact gtggcttaaa agaatataga
    55681 aatgatatag ttaaacaaaa cagaaggtag atctaacttg actatatgaa gaaatgttca
    55741 tattttatta tccttaaatt taaatacatg ccaaagataa aatttaattt agattccgga
    55801 aaagtattgc gtgcagcaga aattttaaag actataggtc atccggtaag acttcagata
    55861 ttagaagcac ttcaggaaaa ggaaccatta tctgtttctg aaattcagga gaagatcggg
    55921 tctaatacag agcagtcgct tttgtcacat catttgataa aaatgaaaga cagaggaatt
    55981 ctcagatgcg agaagaaagg aatgaatgtg ctttaccgcc tggaagacag ggctatttta
    56041 aaaatatttg aatgtatgga gtcatgcagt ctgttctatc gaaaggaata tgtctgatga
    56101 atagaataaa tgaaaaagta attactaaac atagatcaaa taacgatgga aatgttacat
    56161 gcgaagatcc cgaagcaatt agaaaaatat caaatgtaga atcatggaat agtaattaca
    56221 gaaccggtaa catagggtgg gacatcggcg aagtttcacc tccaattaaa aattatatcg
    56281 atcagataaa aaataaaaac attaagatcc ttatacccgg ttgcggaaat gcttatgaag
    56341 ctgagtatct ttcaaaaaat gcttttaagg atattacact tctagatatt tcaagtgtag
    56401 ttacaaatga actgataaag aaatttgcca aagacaaaaa tatcaatgtg atatgtatga
    56461 attttttcga acaggaaaac aaatatgatc taatattgga gcagacattt ttctgtgcat
    56521 taaatccaga tttacgggaa gagtattgta aaaaaatgat aagactttta aacacaagcg
    56581 gtaaattagt tggagtactc tttgacagag aatttgataa agatggtccg ccctatggag
    56641 gatctaaaaa ggaatatgaa gaattgctca aaaaatattt tgatgtaaag gttcttgaaa
    56701 aatgttataa ctcagtgcag ccaagatccg gcagtgaggt ttttataaat ttaaaaaata
    56761 aaaattaatg cttgaattaa taaaacaacc atggcattgg gcagtaagtg gaactgtgat
    56821 agggcttata gttccaatgc tgctggtact tggaaataaa aaatttggaa tatcatcatc
    56881 tttccgtcat atctgcgccg catgtttttc aggtgacata aaatttttta aatatgactg
    56941 gaaaaaggaa gcatggaatt tattttttgt cggaggaata attatcggtt cgtacattgc
    57001 gtcaacattt ctttcagatc ctgcagatat aatacaggta gcaccgggga ctcttgagac
    57061 attgaaagga tatggaataa aagatttcac cagtctaatg ccgttggatc ttttcagctg
    57121 ggataattta ttttcaatga aaggattaat attttttgtc ctcggaggat ttcttgtcgg
    57181 ctttggaacg agatatgcag gcggttgcac atcaggtcat gcaataatgg gacttgcaaa
    57241 tcttcaaatg ccgtcgctca tagctacaat gttttttatg gtaggtggtt ttttttcaac
    57301 caacattctg attcctttaa tttttaaact tattcaataa tgtcaaacag aaatagtgat
    57361 gttcaaatag aaactgctga tgaatttgag atccgtcatc aggatgtctc atgcactaat
    57421 gaatcaaaac tagttcaccc gtggtggtat aacttaaaat atacattagt aggaatttta
    57481 ttcggtatag tatttgtaaa agcagaaatt attagctggt tcaggataca ggaaatgttt
    57541 cagctcacat cattttttat gtatggagta attggtacag cagtggtagt aggtgctttg
    57601 tcagttttca taataaaaaa aatgaaactg aaaagcattt acggtgaaga aatatctatc
    57661 gatgacaaga aatttaataa aggtcagatt tacggagcgg ctatttttgg aattggctgg
    57721 gcgatcacag gtgcttgtcc ggggccgctg tttgcacagg tcggaagcgg attctctgtt
    57781 gtaattgtta ccgtattatc tgctattgca gggacgtgga tttatgggaa gttcagagag
    57841 aagttgccac attgaaaatt aatataaagt attttccgaa attccttttg aggaaatgtt
    57901 tactgaaaat aaataaattt aatttcgttt gtataattat tggttaaata aaattcaaac
    57961 taacttatac tttgaatgat taaagatttt aatttttcag tttcagcttg aattaatcac
    58021 aaaatagtaa aaaaatattc catgaatctg ttgagataaa tgtcaaaatt ttaaaagaac
    58081 gtttcaggat aatccatatg taattcccct taaaaaaggg aagcgaaggt ggctgttgtt
    58141 gaaagaaaaa gtattcaatg aaaggaaatc atctgtctgc aaaaaaggaa attaatcctt
    58201 tcaaacccag tcacattcta aataagttag caataatata ttaattctaa aatccgtaac
    58261 tcttcctgtg acttgtagct acagtatctg cattcaggag tcgtaagtaa ttgttatgtg
    58321 aatgttcaaa ttcaacccag caaaaataat tttcctcttc atttcctgaa ttacaattag
    58381 tgggagtcag tgtacctaac gaaaccttat tatcaacatg tttatatttg actcctaaag
    58441 aatcgagata aagatcaaca gctataatta ctattccgat cgggagcggt acaaatgtgg
    58501 ggatctcatc gggaattata ttattaagac ccgcccaccc ataaggaacg agatctttcg
    58561 aatttactac tctgtgtgac ggagagtttg tatttatcat tacgctgtta taatgttcca
    58621 caaaagattc attgccgaca gttggagctg caaaagtata tgtctcaagc gcaaatatat
    58681 ttgtatatcc attatctaca aaccaagcag aaagcatcgt agcaagtgca ccacctaaac
    58741 tgtgaccggt gataaacatt ttcagattgc ttccctgaat accatttaaa aatgctgcaa
    58801 gatttttacc gttgtctgta attgccagta aagtattaag accgtcaagt gaaccaactg
    58861 ctactgaatc caaatttccc gccgtaaaag gatatctgtc aagtttccat acttccatat
    58921 cttcttttat attcgatgga aaaatccagt ctgttcctct tatagcaata gtatataaag
    58981 aggaatcacc ggtagtatca gcagcaacat atatcatatt ccctccgtca ggacttatcc
    59041 ccggtcccca tactaatttc cagtttccac ctgtagtata atttgtattt gccaactgaa
    59101 gtattaaaga atcttttatt tcggcatggt taggattatt ctcagctgta taacataatg
    59161 ccgccagagt catgatctcc cctgcttcat tttcttcata cccaccgagt gccggcggat
    59221 ttccgctatt aatacttgtg agatcatcag tacaactgtt aaaagaaatg atgaacagcg
    59281 tcattgtaaa aatgaccaat gcagatttga gtaatgtatt tttcatttta aaaaattttt
    59341 gaaaaaacta acagcattta ttttttaatt caagtgataa gacaggggaa atttgaaaac
    59401 tacattatct aaattttgtt ttgtatttca gattaatatt tattttcaaa acagatttcc
    59461 gataagttat cacctgcggg cgactttttt tgttactttt tatgtcgttg aaaaaagtaa
    59521 gtagaaaatt ataaaacttg agttttggga ataaatgttt tggacggacg tattttatga
    59581 agaataatat tcttgattta ttttcgaata aattttatgc tttaaaatag atcatttttt
    59641 attatgttaa taataatcaa ttattattcg aaaaatattt aacaaacaaa aatgaaaaat
    59701 tttattctga tcatacttct tacaattaat ctctctgctt tttgtgacga accatatatt
    59761 gtgtttcccg atgttatgaa caaggtctca attttagaaa atacagatgg tgttcaggaa
    59821 aaaaagtttt atgatgctga ctcattgcct gtatttcccg gctttccaaa gatcataaat
    59881 ggtcagtctt ttgaaggcgg gatctattgt caaatggatt ctgacagtga acttgaaata
    59941 gtctatggta tcggattgtc tgttcaggca tggaatattg acggaagcaa cgtaaccggc
    60001 tggccaaaga gtctttcatt taatgcacag ggagctccgt ctttcggaga cattgacggc
    60061 gatggagttg aagaaattgt aattggaact gcaagcctga caggtactac aggttcactg
    60121 tttgctttcg aaaaagacgg aacgcctgta acaggattcc ctgtatccat cggctctgcc
    60181 agaactgtaa ctctctgcga catggacaat aacggcagtc tggaaattat caccagtaaa
    60241 agactttctt cagcgggaga aatttatatt tacaaaggcg atggtacggt ttttccgggc
    60301 tggccaaaag caattaatca tgtacctgct tcgagttccg ctgcgggaga tataacagga
    60361 gacgggtttc ccgaaattat ttcggaatct tatacaagta tttatgcatg ggatagaaac
    60421 ggaaatgtct tacccggatt tccttttata cttcctaatg gtgatgtgaa ttcttattca
    60481 tctcctgttc tagccgatgt cgatggagat aatatcagag aaattatttt cggctcacat
    60541 gtcagttcag caggtggttt aatttatatt ttaaaaaata acggtacggt tttgccgggc
    60601 tggccaaaga gcacaaacta ctgggtctat ggcccgccgg tacttggata tttaaatgat
    60661 gacaatgtga tcgatattat tgtcggtgat cagatcggca gcggcactcc ggttaacttt
    60721 gtttacggat gggataaaga tggaaatgta ttaagcggat ttcctatcgg accggtaaat
    60781 gcggtaaata atcaggttgc tcttgctgat attgataatg atatgagact cgaactttta
    60841 atagatgaca attctcagac cggaggaatt ggaaaatacc ccgcattcaa tcatgacggt
    60901 actccggtca tggattggga cctgacggtt aacggaactt ctttttttaa tatgccctgt
    60961 cttactgatc tgaattcgga tggaattctt gacattgtcg gtgccggatt tactttgttc
    61021 ggaagtaatc agactagtgt ttatatctgg aatgcgggaa taccttatga tgcagcaaaa
    61081 atagtaaatc ccgtctttca gtttaatgta agacataatg gagtctacgg tgacaataat
    61141 ttagtatcag ttttaaatta caacaataca atacccgata attttaaact tgaacagaat
    61201 tttccgaatc ctttcaatcc ttcaacagtg ataaattatt cattgccttc agagttcaaa
    61261 ggtaatgtaa aactgaatat ttataatgca cttggaagtg agatcgccga actggttaat
    61321 gaaagtcagg gtccgggaaa ttattccgtt gaatgggaag cagtcggatt tacaagcggg
    61381 atatattttt atgttttaag atccggtaat tttaaagagt caaaaagtat gatcttgtta
    61441 aagtgattaa aacgtcaaag tagttagtga taaattaaat ccgaacttta agaccgcaga
    61501 gtacaattaa agaaattctc tgcggatatg atcagattaa tataagatgc agctgatcat
    61561 tttgaaaaat cattacagtt aaaaattatt tgaccagcat cattcttttg atctcactgt
    61621 agttttctgt tacaagttta taaaaataaa tactgctgga caatttactt ccgtcaaatt
    61681 ctattttgta gcttcctgaa ttctgtcttt cgtttatgag agtagcgact tcatttccta
    61741 aaacatcata gaccttcaaa ctaaccaatt taccggactc ctttagatca taactgataa
    61801 tagtttttgg gttgaatgga tttggataat tctgatttag ataataatct ttgcttcgta
    61861 tttcctctgc agaaatgcct gtaagggtct gagtatattt tatagttact gcttcataac
    61921 cggataaacc cgttcccagc ctgcttcttc cagtaacata taaattactg ttattatcta
    61981 ttgctatcga tgaaccaaag tcaacttgat tagccggacc attatatctt attgaccata
    62041 aagaatctcc tgcagaatta tattttaaag taataaagtc aatacttcca ccggtcacat
    62101 atatatttcc caaagcatca caaacaatat cttttcctcc cattccattt agcggatcat
    62161 aataatttct ctcccactga aaaactcctt cagaactata tttaagagtt gcaaaactta
    62221 ccggtcctgc gttgtaaatt tctcctgtga ttaagacatt gtcatcttta tccaggcaga
    62281 ctgcattaat gtcatcatga gtagtaagta atcctccaaa tccatttcgg atttttgtcc
    62341 ataatgtatc tccgtccgga tctaatttta caattacaaa atcatcaaat gtagaagtaa
    62401 acttttttgc gccaaaataa atatttccgg aagcatcacc ggttaaaact ttgtttgatt
    62461 cagatccact gaattggtat atcctgttcc aaagttcatt tccgttagta tcatatttta
    62521 taattcttgt tcctctgcca tatacatata catttccatt gtcatcagca taaatatcct
    62581 ttggaatgtc cgtcccgccc agtgcgaatc tttttatcca taatgaatct ccctgcggac
    62641 tgtatttaat tgtaacaaga gtcattggtg tagaaccaaa tgaatatccg cttactaata
    62701 cattattatt attgtcaata tccatcgcaa ccggctgatc cattgtgaaa gcagtttcac
    62761 cgttatacct gttcccccag agtgaatctc cgtctgtgtt atatttaatt gttataatat
    62821 cataagtacc ggtgagattt tcggataatc ccgtgacgca gacatttcct tcattatcaa
    62881 gagccatatc aactacccgg tcttcgatac ggaatgaaaa tttttttgtc cattgtaaaa
    62941 caccggctga attatatttt attgtcacat aatctgatag agttgagctt gagacagtac
    63001 ccgaaataaa tacatttccc attttatcaa tcaaaggtcc ggataatgta ttaattgttt
    63061 ctgtattgct gccaaatttc tgtacccagt cctcatgcac tatctgtgat ctcaacagta
    63121 agtgaaatga aaagaatatc agaaatgaaa taattatttt ctgcaatgta atatttttat
    63181 atatactgat ttaatttgtt tattcaatta attcaattta tttaatcttt tcagttaaac
    63241 aaaatttaaa atgtaaggaa gtgtagaatt ctgaatattg tcagcgggta gggagagatc
    63301 aattaaaaat taaaaattac gagttaatag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63421 nnnnnnnnnn nactattaac tattaactat ttaatctcat cagctttttc gatcagccat
    63481 aatttcattc caaagtgatt gctgttaaga ttatttatat catatatgat ctttgatact
    63541 ttttccttat cctttaaatc tgatgaagtc ctgagtttag taagttcgcc gatgacacgc
    63601 agaaaattca gaagggtgtt ttttaaaact tccgttatca ttttttcgcg cgagatataa
    63661 tgtctgtatg aatctatcat taaaaacacc tgatcataaa attgtttttc atatacaagc
    63721 tgaatgacga gcaactttac ctgtaccttc attatataat tggagaaatt tgtttttgaa
    63781 aaaagttcaa gcgccaggtc aaactctttt tttccgtacg caataaaacc gtaacaaaaa
    63841 tttaaagtgt tttctttttc ttctgtcaga ttttttttaa aatcattaag atacttttcc
    63901 gcaaaactaa attcatttac ccgaactgct ttttttatgt catttaaaaa atcagggtaa
    63961 agtatttttc caagttcggg aaaatgattc atattacatt ctttactcaa tagatgctga
    64021 tcaaaaagaa gatcagtcct tccattataa ttatattcag tagcgcagta actgtcgaga
    64081 tgcagaaaga gcatatatct gtcggcagaa ttcagttcat gattatattc ttctcttaac
    64141 tgtttaagtt ttaaataatt ttcttcagat ttatcgagac aaagtttaat gatctgataa
    64201 taaactttaa tggtaggatt tgtttcttca ggattatttt caatgtaact cagtacctgt
    64261 tcaaacatcc gcatgtcata tctataatta ttctgattta tctcatgaag catgatattg
    64321 taagtcttta aaagcataat tacagaataa cttacaaaca gattcagctt gtcctgctga
    64381 aaatgatggt tctcatttgg ttttctggga gaataaaaat ttgttaattc tttggtaaga
    64441 gtaaatttat gaaacaaata attctcatcg gttattcttg atttttctaa acgggcagtt
    64501 gcagatttaa aagactgttc atagatttta tctagatttc ttctctccag ttcagacaat
    64561 aataaggatt cctttatttc gtcactagtt ttaaatctct gaaataccag gaaatccttt
    64621 ccgagtgaaa gaagatcgga aacgagattt ttcattttgt aataatcata tttggaatct
    64681 ccaaacaact ttttaaataa attttcttct gtcagatccg tgtcatcaaa ctccgggtaa
    64741 tacatttcaa gcagatcata taagcttttg atgaatttat tcttattaaa aaccggggat
    64801 aatataaaat ccctgaagtt tttgaattct gacggattaa gagaactgag aaattgtatt
    64861 aagcgggttt tctgcattca gtttgagtaa tgtctgttct aaagaattgt tacaaaatac
    64921 attataaaac gtcaaaatta aagtattttt tgactatttt attattttag tctttggata
    64981 atttctaaac acctgttttt ttttctttgg gaaaatgagt tatcattatt attttgtcta
    65041 aaagttttgc tgtatggaaa ttttactatt aaaaattccg gcgaagagat ttttaaatta
    65101 aaatttattt tctgaaaggg aaaaaatgaa aaaaactatt cactgtttga tcttaatctt
    65161 agctttaaat gtaattagtt ctgcagatgt attttctgca aatgagtatt tcagaagcat
    65221 tgcaaccggt aactggaact ctacctcgac atgggaaatg tcattgaata attccacttg
    65281 ggtaccggct actactacac ccgatgctac tagtaattta ataacagtcc gatatccaaa
    65341 tgttgtaaca gtcacagcta atgtaaacgc tgatcagttt acgatcgaca gcggtactgt
    65401 atcgatcaat tccggaataa ctttgacact tcttgacgga agcagtactg attttacaat
    65461 cctcaaggga ggaacagtca caggttcagg tac
//
LOCUS       DAORVQ010000003        52776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_162_length_52776_cov_6.421654, whole genome shotgun sequence.
ACCESSION   DAORVQ010000003 DAORVQ010000000
VERSION     DAORVQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 52776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52776
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_52776_cov_6.421654"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1592
                     /locus_tag="PLX80_00585"
     CDS             135..1592
                     /locus_tag="PLX80_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19320.1"
                     /translation="MINYKAINILKGLTKKEILRFEKFLLSPFYNNNKKLLLLYNYLR
                     KFYPEFDSSSFTKENIYRHVFGNDIFSDENLRKLFSDFYKLGEKYLVTLNLEKNKFDY
                     DRYLLDELDWRKIDNIFLSKFKEKEKQFEKSEFNYQTFYYDYLMKWQIVSFHLDRGEQ
                     YKITGDVFERTEQLIFYFLSDLFISLNDIDVNKNTFNYNPKINLPEKFIELLDLESLY
                     QYIRDNNFKNKDLFSMYYNLFLMNKNRTDESYFFELKDQLEKNFERLSIRSKMHFILR
                     MISMCNRYINLNKSTKFHEIKLDLYDKILKYKLYKTNQNYFRSDLFLNLFIEYISYKN
                     TSDAELFFKENIKSISPAHIYKLQSLCSALLLFEEGKFLESLEICSRINTNLLTVKII
                     LRKLLLKIFYEVKDYDGNKDEIKNFRQFISNSKLLNETHKSSLQNFIKLFNDLVNLKN
                     DPEDEFLNYTLKSNTEHIIDTSDRDWFRNKISELN"
     gene            complement(1638..2945)
                     /locus_tag="PLX80_00590"
     CDS             complement(1638..2945)
                     /locus_tag="PLX80_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY19321.1"
                     /translation="MNLKKILVISYYWPPGGGAGVQRILKFVKYFPENGFQPFVVTVD
                     EEKASYPVIDKSLNAEVPHEAEVIRTGTFEPFDIYSKLIGKKSIPTGFSNETDPGMIQ
                     KLSRFIRGNFFIPDARKGWIKFAFTEACKLIEKEKIDIVITTSPPHSAQLVGLKLKKK
                     YKINWIADLRDPWTDIYYYKEFKHLPFAEKLDKKYERLVLENADKIITVSNDLKRMFL
                     QKSDSIDEKKFTIIPNGFDEDDFKNLKKPDKTQNDFIISYTGTLAESYNPQIFFDELK
                     KVVTENPEINIKLRFIGSPADSMMNRIREMSMSSNLEVIPTVSHERSVEYLNESTLLL
                     LVIPEVKNDKGILTGKLFEYLASGKTIVCIGPEDGEAAAFISECKAGKTFERNDEYGL
                     SEYLSGLVQYWKVKGDLNIENDLYKKYSRRSQTKYLCEVLKCF"
     gene            complement(3164..4495)
                     /locus_tag="PLX80_00595"
     CDS             complement(3164..4495)
                     /locus_tag="PLX80_00595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY19322.1"
                     /translation="MNEISDTPLKTERSVLIFILSCAFLIKLILLFLLDPVLRSDSLD
                     YQTLANSLLNGEYSLNGKPTAYVGIGYPAFLSGIFFIFGEGQFYVRLVQSVIEAGTAL
                     LFYGVSRRFFSIKHSLISVAIFSFFPSNILYSQTILSEPLFGFFSMAVLYLFLRDDFS
                     RKPIQIFVAGLIFGLAVLIRTAYLPICILVPLFFYSYREEIFKTSKVRSAVIFSMIFF
                     MGMILVIAPWSIRNKMMVGTYSLGTTSGLNFWLGSNPDATGSYFIKPDDMLPGDYSNE
                     ADRDKEYFKLGLDYAVSHPVNYMILGIKKIGYLYSSERMIVNYFTETLPGQTSTDVYR
                     SASPLFTLLVNIPYFIIMLLGTWGLMYFGRKKFFIYGSVLIWIFTVVVFIALARYHYV
                     LIPFFILGTVMFLSLGRGSLNEISTNKKILAAGFSLFLIAVWTVEFYLMMR"
     gene            complement(4501..5154)
                     /locus_tag="PLX80_00600"
     CDS             complement(4501..5154)
                     /locus_tag="PLX80_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQY19323.1"
                     /translation="MYIPKHTKMKNEDEIFDFIESNNFGILVTLNDSKITATHIPFLL
                     KKGKEKYGELYCHIAKANPQHQNISGEVLVIFPGAHKYISSGWYESDQTVPTWNYMSV
                     HVYGELQVIIDRDEKFEIIKELVDLFEPDKDIYSLDNLKPEYLENLLKGIVAFRIAIS
                     SVEGKEKLSQNHSMQRQKIVIDKLTEIGDTDSLLIADRMKINLSTDKISGTDSPNIP"
     gene            complement(5174..5980)
                     /locus_tag="PLX80_00605"
     CDS             complement(5174..5980)
                     /locus_tag="PLX80_00605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19324.1"
                     /translation="MKYDPVKKVFGDIVSDNSFFRKIFYWLLDLMFLRSWHVRKKVRE
                     LYPKGKVMNIFDAGMGFGQYTYFMAKRFPDSDILAIDVKEDQVDDCKRFFSKCGFKNV
                     RFEVGDLTKIDYNRQFDFILCVDVMEHIVEDELVFKNFSRALKKGGMLLVNTPSNLGG
                     SDAHEEDDESFIEEHARIGYSKEDISEKVNRAGMEVHSFDYTYGKYGTISWRWGIKYP
                     ILIAGVSKFLILLLPLYYIFTLWFVLIFMWLDVNTYNKEGTGITMVSKKI"
     gene            complement(6031..7179)
                     /locus_tag="PLX80_00610"
     CDS             complement(6031..7179)
                     /locus_tag="PLX80_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY19325.1"
                     /translation="MKIIIVGTAFPLRGAMAQLNAILANYLSKDHDVEIFSFKRQYPE
                     LLFPGKTQMDTSDNDILNKDIPNTNIIDSINPLNWQKTAKMIAAKEPNLIILRFWIPF
                     FAPAFYRICSYVKKKTGAKILFICDNVIPHEKRPGDNFLIKLIFKVGDYFIIHSKSVE
                     EDLKKFIKGKPYKYTPHPIYNIFGDPISKEEAVKNINKDFNIDLIGKKVILFFGYIRK
                     YKGLKYLIDAMPEILKSIDVTLLIVGEFYEDEKITHDHINSLGLEKNIHVVSDYIPNE
                     KVRYFFCSSDVVVLPYIDATQSGITQIAYYYDKPVIATDVGGLSEAVHDGETGFLIKP
                     KDPKSVAKAVIKFYKEDLENKFSENAKDEKKKYEWGRMTEAIEELYGK"
     gene            7311..8993
                     /locus_tag="PLX80_00615"
     CDS             7311..8993
                     /locus_tag="PLX80_00615"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HQY19326.1"
                     /translation="MAENNLEKLISKKLSRREMLKTSGKSAAVIALTSIFPVGFLNLL
                     SKNANSLTTDFSVPFIPIFPSSEDKLFLPKEFEYTIIRKWGDEIADGVPYGFNNDFSG
                     YVPIDFLSGGNNSEDGLLIVNQEYPSPLFINNYSDEDFRNGRIKTSEEVNKEKLSTGI
                     NIFHIKRIDGKWKFVNDNRYNRSINGTTIIKLSGKTAGSEEMKFKEFASGTLANCSGG
                     ITPWGTMLSGEENFQDHYASDNIWEYRWNDADKDFIIEHYGWVVEIDPFDINSVPKKR
                     TSLGRFRHENVTVGISSSGKAVAYMGDDKVNECVYKFISKNNFNPDKRSANMDILDEG
                     DLYVADFLKNKWQLLDFEKRDDLKSIFKSQADVLVNCDKAAKTVGGTECNRPEDIEIN
                     PKDRSVFISFTNNSKKEDYFGSIVRIMEKDNDPESLDFEWEVFAKGGADTGFSCPDNL
                     TFDNDGNLWVLCDISTSKINKEPYSEFKNNSLFMIPVSGIDKGKAFLFATGPVDCEMC
                     GGTFTPDGSTMFLSIQHPGEGSLYYDQPTSRWPEYGDDIPRPAVVAITGFKN"
     gene            9042..9848
                     /locus_tag="PLX80_00620"
     CDS             9042..9848
                     /locus_tag="PLX80_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19327.1"
                     /translation="MDEVYKFFETNKNAWNKNTDVHITSEFYDNENFINGKNSLKHIE
                     IEMLGDIKGKSILHLQCHFGQDTISLARAGAVVTGIDFSDNAINKAKELVVKTKTDES
                     TEFICSDLYDLKNNLDKKFDVVFTSYGVIGWLPDLKKWSEIISHFLKPGGKFVMVEFH
                     QMLWMFDDDFNYFKYDYFNRGEVKETSEGSYADRNAPIKSDYVMWNHSLSDVINNLIK
                     SGLEINSFEEYDYSVYDCFNKAVEYEQGRYRVKGIDVCFPLMFSVTALKK"
     gene            complement(9862..10758)
                     /gene="era"
                     /locus_tag="PLX80_00625"
     CDS             complement(9862..10758)
                     /gene="era"
                     /locus_tag="PLX80_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQY19328.1"
                     /translation="MVKKSGFVTIFGAPNAGKSTLLNALLKFNLSIVNKKVQTTRDRI
                     LGILTEEDYQIIFIDTPGTLEPKYELQKFMLREIKTSLDEADVIIHIVDAAKLNLERL
                     KKTDEEFIEILKDKKRIIVLNKIDLMDKGKLLPMIETISKNFGYDDVVPISALNDDNL
                     DSLKKVILEYLPESEFYFGKETLTNKSEKFFVSEIIREKILRYYHEEIPYSILVNVTE
                     FKERSEDLVYINADIVVERETQKIIIIGKGGSGLKNLGNKSRQEIEKFLGRKVYLELF
                     VKVRKDWRNNKRFINDNLSGDV"
     gene            complement(10762..11280)
                     /locus_tag="PLX80_00630"
     CDS             complement(10762..11280)
                     /locus_tag="PLX80_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19329.1"
                     /translation="MKRLKIYCSALNNLFKFTLIVCLIIISGCSDENKNDKQNDKVEI
                     KNFSDESSSTAENEIEDEEFGEDTESDISGNSESTVTAEKVKDHLGEIITVKGIVSDV
                     HISEKVAYLNFGNKFPKNDFSGVVFSGKFSEFDDLESFKDKLVEITGSVSVFRNKPQI
                     ILNSSDQIKIIK"
     gene            complement(11798..13312)
                     /locus_tag="PLX80_00635"
     CDS             complement(11798..13312)
                     /locus_tag="PLX80_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY19330.1"
                     /translation="MAEIFKNLINGEWKNSSGSETFENRNPAKWNEDLIGIFPSSNKD
                     DVNEAVSAAKAAFDKWRLVPAPKRGDILKVVGDKMSERKDEIAFEMTREMGKVFAETK
                     GDVQEGIDTAYYAASEGRRLFGLNAPSELQNKMNLSFRVPIGVGGFITPWNFPMAIPT
                     WKMFPALVCGNTCVFKPAELTPKTAATLIEIIESSIKEVLGKDYIPGVVNIVHGKGSI
                     VGEAIISHNDIDLISFTGSTKVGKRINEVAGGDLKRVSLELGGKNAQIVMNDADLELA
                     LEAVLWGAFGTTGQRCTATSRLILHKDIHDEFLKMLSEKAKTLKLGYGNDPDNQIGPC
                     ISESQRDSVHSYVKIGLEEDKATLLCGGEFATEDELADGWFYKPTIFTDVKPGMRIEQ
                     EEIFGPVLSVIKCNDLEDAINILNDTKYGLSSSIFTKNINDAFKAIRDIKAGITYVNG
                     ATIGAEAHMPFGGVKQTGNGHREGGWTVYDFYTEWKAVYIDYSGKLQRAQIDNY"
     gene            13508..14029
                     /locus_tag="PLX80_00640"
     CDS             13508..14029
                     /locus_tag="PLX80_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19331.1"
                     /translation="MKRSELASLPVYFGDYIKEVEDIDLISALVKYGPEMFVKDLPTL
                     EKIGGNTYAENKWTIKQILQHLADTERVFLYRALSFARKDETPLPSFDENLYAEISNS
                     NRRSLNEILTEFESIRNSALTMFQGFTEEMLLSTGNCSGKEASVAAIGFTIAGHTFHH
                     YKVIRERYFLLAD"
     gene            complement(14401..15891)
                     /locus_tag="PLX80_00645"
     CDS             complement(14401..15891)
                     /locus_tag="PLX80_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY19332.1"
                     /translation="MKTILYFTFILISTMSFTNQRGIILYTDATTTNLPIQAVTGPTM
                     DAAAGDLDNDGDPDIVLAREFAPNKILINNGNGQFTDGTLGNLPQFNYDSEDIGIADF
                     NRDGFNDIVFASEDNAIHELYFNNGNGSFSNLNNRLPGSTANAVLAEDINNDSLPDII
                     LGNAGQDLILINNGDSIFTNETSLRLPAESNVNQDLKLADIDGDGDKDLIAGNEDGNK
                     IYINNGSGVFTDDTEQRLPPSLTEETRKVTLGDVDGDQDLDIFFANVAFISGRTRQDR
                     LLLNNGSGFFTDITSTNLPVDNEQTTEGIFIDIDYDNDPDLITSNIFFNRPMKIFENN
                     GSGIFTEVTDIVLPQNTVAEGIGVLGTDLNNDGLMDLYLQNRRTTQQTTETDRLLIRN
                     DTGAVQIFNVENEIPVRIKLHQNFPNPFNPETVIRYELNESGYTEIKIYDIQGKIKGV
                     LVNEKQGSGEYSVKFDGSGLAGGVYYYRLTVNGGEVVMTKKLVLLK"
     gene            16034..16507
                     /locus_tag="PLX80_00650"
     CDS             16034..16507
                     /locus_tag="PLX80_00650"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HQY19333.1"
                     /translation="MHRKTALIFILFSLVYFAPGNKAVSQDKWWKEKKFKNEHLRAKY
                     DLCKRTFKDIGLGFSISNVNHINQYFDEQVYLNIISTDKGYYSPNQAEIIISEFMDYF
                     NVDEFKFMTSKRYNTYAFVNGKYSYRLGNGKRTLDVTISLKYLNDKWYVDQININ"
     gene            16615..20694
                     /locus_tag="PLX80_00655"
     CDS             16615..20694
                     /locus_tag="PLX80_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY19334.1"
                     /translation="MILIAFLFIAWLAVANLFSDYIKSETEKNWDEISKEKNEQRVQI
                     VNRLFLSDLDNLNELSNNISKNSEIIDLIRKSESKKLFETLINLKLSNNTQYEFYNSR
                     LELLAFSGRKLGSDFYSLNKSINGKRFSVLKEIGFYTYLIIYSPVYDPESQSVIGVSL
                     TAELIDIKYQIDNKFFKNSGLLYSISKETSVSPELFPSNVISGKIEVNSPNPEDFIQI
                     DLKGLENEIIGVILIPKYNELNHVSELHDFTLRLNSILIFGLSVIIFLLTYKFIFKIN
                     SSLLKFILFSAVLIFFRYLWLQFDFPSKTFNSDLFSPGYYASVFGNGIAKSIGELFVT
                     SVVVLIIALFGFNTVLRNKFVSKNNFNFLKALKIIFSVILFFALIYGFGAVIQSVVFD
                     SNLKFFDKSNIIPGPELFFIQLVILILTVSFFIFLLSLIIHIYKYSLFPVFSKKTIRN
                     FAPLFILLILLLINQIFTVLDMNFYINTLFRIIIIVSVFLFGSYLGWKLYKFKIYNVY
                     SLKNFSVLILICIITIPGILLENITSQETRYVELIGNKIADKDDDKIKFLLLTELSDI
                     SNDKKIENNIRNKNKLKELGFSIWNESKFSEENLNTSVIILDTNRKVVSDFMFNSNAM
                     ILDSIIAFANKEYFIKKESLENIPDSSDFTDSLSFADDPENEEEMDFSEESFKEDLNN
                     LFITDKILILNNEEENYYLGIVPVERVDLKNTIYETNLGYLLVAVQYESKNLLQQSSV
                     QIFKTYSRDNLFDKLISEPVITEYVNGEIVSSTSRDQSKSNTLSLNVFRDAVKFKDEK
                     SDWRYEVINNEKFRSFYILDRNIDKSMNERIYSVSLKRNDVKLTFFFYLKFTLFAFLI
                     YMVVFVIKLLQIFFSLKELKPNFREKLFASFFFVSVIPIVLLALYTRSFIKEKYDINF
                     QNQIISDLNLVSQSLKNLNLNQLSSDSLSVRGKKIFTANLFPSDKNFNLYINTDLVST
                     TNDELYKSDLLDSRVDAEAYYNINYMRKDFFIKNEEIGAYSFIVGYKPYFDQKNNVVG
                     IMSSQTLYKQNELSEELTEILTFIFGIYLIVVIILLVFVSIMTDRFSQPIMKLKYATE
                     RISKGDSNVAIDIKRKDEIGNLVDSFNIMSRELESSKVKLKRAEREAAWRDIARRVAH
                     EIKNPLTPMKLSIQHLFDVYENKTPEEFSEVLKKTRNIIINEIDKLNKIATEFSDFAK
                     LPGKNYTETDLNEIIGEVISLYKLSPNIEFVQDLNSNIGFISADKQELNRVFQNLVKN
                     SVQSIGVNGKIEISTRENGEYIIAEVKDNGCGIDPSVMKNLFNPNFSTKTTGMGLGLS
                     ITKKSLDDMKAEIMFESIVNKGTTVIIKFKKINSKTNNS"
     gene            20735..22090
                     /locus_tag="PLX80_00660"
     CDS             20735..22090
                     /locus_tag="PLX80_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQY19335.1"
                     /translation="MNFLFSCANQLPPSGGEDDTNPPKLIYIFPEPNSTLVKGNKIIF
                     KFNEYVDRRSFEESFFISPRSAGDYSFDWSGKEVEINFEKKFEPDRTYVMIIGKDFKD
                     QRGGNTLSSPVTIAFSTGSRLDKGTFSGKVFAGNYDRVKVMIYFSKDTTSVFSDPANS
                     QPDYITQVNLDGTYEFTNLPNGKFRLYALTDEDRNNVYDSDLDKIAMLPDDIKLFGDS
                     TSVSDLNFIMDLKDKNNYSSLFLNTLSSDSSGLIYSNYNYSGSNFITPEHRFYFYFKN
                     ITPDKKEVVENFILSDTSDRKVYKPIFNWINDSLLEVFTQEKLKFNSVISLAIDIKGT
                     GNSRFTGSYNVYEKDKFGKISGDVKRTDPDIPAILTLYSLAKDGPIYTKVISDTTAFE
                     IDDILEGKYILFVFIDNNLNGTFDKGNVNPFKAPENFIIYDKDFEIKGNSSIEKILLE
                     F"
     gene            complement(22113..23246)
                     /locus_tag="PLX80_00665"
     CDS             complement(22113..23246)
                     /locus_tag="PLX80_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19336.1"
                     /translation="MWNNIIGQERVKEIFTNFYKGGKISHAYIFYGNEGTGKDAAAVE
                     FAKLLNCDNKVNDTEACDICKSCIEIDSLKSPMLRFITALPTGKSDSDEDSNPLEKLD
                     KEDFQIYLSQMELKAEDKYHTISIPRANDIRISSIRQMKREIYMTGRSGKKKVFLISR
                     CDMMNPQSANSLLKILEEPPKDSVIILTTSRLNSLLPTIIGRCQKIGFESIQKKYIID
                     YLKKKKHSISDENAAFFADISDGSISRCNQILENNYLDLREKVMDTLVALLTVNNLKL
                     SSSIDFIIGKRDKVRIKNFLIFLIIWFRDIINFDNGNEELIINRDKKERLERFTNNYE
                     SDNYLIICSIEDAVKSLDNNISPELLLYNLFFTIRSFINKKNN"
     gene            complement(23248..25275)
                     /locus_tag="PLX80_00670"
     CDS             complement(23248..25275)
                     /locus_tag="PLX80_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQY19337.1"
                     /translation="MMNNFLRLFLITGILFLYTGMSECQEDTSIEGNYEIDDINIKFI
                     ESSTFDESTIKGLLASKSGDYFDMTTFLLDVERIKKYYFDNGFFDAEVDTGLFTNIED
                     KEINETFTVKENARYEYLNINYTGIDSISADLTGKIQNQKNSFTKKGRYYSKDSVNLE
                     RGRIMDLLQDNGYATASALNPEIFKYVTNIDSLFYKVNIKFVLDPGKKYEFGKTVVKF
                     TNDKYNITEDDIRRELEYKENEMYDKSKIVKSEINLTRFSILENPRIVIDTINENSNK
                     INFIVNAVVGNKYDVTPEVFGYYFQNSFYMGTGVSFNDLNFFGDGRVLTSRARFYYNS
                     PKNNRLEFVNTISQPFLFGNRFITGNWNIGYEYRLQDDIIQSIFKNSFNVVYELPDYT
                     YVNNLQFRWELENSRFVLNNDIVSEEEEILLPNFDLNEFISKFGFTIIHSSVNNIVFP
                     SAGNYQSYEIEESGLLSGVIRKIFNTASYSYSKFTNLSSAYINLSNREVNVTAVLAFK
                     LLAGIIAEYGQNSFTFLGQEFSSDRVPRDTRYFGGGSTSVRGWAARQLGIVPDKDLGG
                     NFTYENTVEYRVKPFLSMSNEYLRDLGFVGFVDYGNVWSDIGKFKLNEIAIAAGTGIR
                     YYTIIGAIRFDVGFKIYDPQPGPIGGSNWIFAPGSNFNDKYNFQFGIGNTF"
     gene            complement(25339..25566)
                     /locus_tag="PLX80_00675"
     CDS             complement(25339..25566)
                     /locus_tag="PLX80_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HQY19338.1"
                     /translation="MVWTLELASYLDDAPWPANKFELIDYSIRSGAPNEVINNLNDLE
                     DTEEPFESIEEIWLDYPTDEDFFYDEESKDF"
     gene            complement(25650..26762)
                     /locus_tag="PLX80_00680"
     CDS             complement(25650..26762)
                     /locus_tag="PLX80_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY19339.1"
                     /translation="MKQPNEINEFIEKNIDDKPDPNGMSKSQLESQKEVGVSYWSLVK
                     HQFKKNKLAMISMYIVLFLIVIAVTADFLADSKPLYAEYKGETYFPVVKDYLNAIGIS
                     KWEPDMINANWKKLDDEGKLESVVWTPVPYGPSEIDLANNLRSPGGDHYLGTDAIGRD
                     LLAGLIHGSRVSLSVGFVAAGIALVIGIVLGSLAGYYGGKVDLIIMRFVEIMVTLPVF
                     FLIITIVAIYGSSIWYIMAAIGITSWTGDAKLIRGEVLKVRNMEYITAASSIGLPNKQ
                     IIFRHVIPNAIAPVLVSGAFAIAGAILTEAALSFLGFGVSATTVTWGSLLNEARGASN
                     AWWLAIFPGMMIFIAVVTYNLIGEGLRDALDPRLRD"
     gene            complement(26770..27786)
                     /locus_tag="PLX80_00685"
     CDS             complement(26770..27786)
                     /locus_tag="PLX80_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY19340.1"
                     /translation="MTDYVIRRILLFIPTLIIITMITFLVCRLAPGDPTEMKIGQTQE
                     AQKTDSKNLMNEQAKEFYKKKFGLDKPLYMQYFIWIGNMLQGDFGNSFKDDRPVIDKI
                     LERLPVTVTISLLSFLLIYLIAIPIGIYSAVRQYSFTDRITTVFLFVLYSLPNFWVAT
                     LAIVFLCNVEYLKIFPTAGIRSEDFDSLGFMGQVKDRFMHLFLPVTISSLTSFAFISR
                     QMRSSMLEVIRQDYIRTAKAKGLSEKKVVMKHALRNSLIPIITLLGGLLPAMIGGSVI
                     IETIFSIPGIGQLAFQAILDRDYPLIMAELVLTSVLTVVGILAVDILYSFVDPRIAFS
                     KKTA"
     gene            complement(27860..29665)
                     /locus_tag="PLX80_00690"
     CDS             complement(27860..29665)
                     /locus_tag="PLX80_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY19341.1"
                     /translation="MYNPIKVILPMMIIFTLIFSSCGRNKETTESKENATVDLSKLAP
                     KNIENADDGDWVIKQEMSDAEKLNPTVTNDASASGIYIYIFETLLDVNRETYDLVPLI
                     AKSLPEISEDKMSYTFDLKEDVKFSDGTPLTGEDVIFTVKTIKNPFTDAQALRNYFID
                     LKSVELVDGNKYKVRFNMSKPYFRAVYALGSMAITPKHILDKDNMNEKFTWEQLDEAQ
                     KSLDPLKYPDMQKYADFLNSQEVSREPRYVVGSGPYKLDKWLTGQSITLQRNEDYWNK
                     KDIPNFPSKLVFKIIQDQNAAVVAAKNKEVDYMYVIQPIDFVENIKDPEKFNLKKALV
                     SEPVYIFIAWNNKNPLFADKKVRWALSYAIDRQTMIDKIVYGMGTKVQTPVFKESKYY
                     NNDLPEIDYNPEKAKQLLAEAGWKDTDGDGILDKVIDGKKTDFKFTFINNNNPKRKKV
                     MLVVIESLKNLGIQADLQEYEWSVFLDKTKKHEFDACYSAWQLSVTPEDPYQIWHSSQ
                     AIGEGSNFISYINPESDKLIEQNRVEFDDAKRMELLKKWQEIIYEDQPTTFLWAEPSR
                     YVYSDRFNNTRWYAYPDSPLLNEWWTPTNAQRYKN"
     gene            complement(29706..30752)
                     /gene="hprK"
                     /locus_tag="PLX80_00695"
     CDS             complement(29706..30752)
                     /gene="hprK"
                     /locus_tag="PLX80_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HQY19342.1"
                     /translation="MPGRFENIKEIKKNFITVEFLYNEFKGRFKLEKISNEEIDTRLQ
                     ITEKDIHRPGLALAGFVELFTFNRVQIFGNTEIHYLEKLSPQDRLKSLQVFFSFDVPC
                     VIITNNNKVPDELIKLADEKHIPIFRTVYPTTKFSYFISDFFDDQFSTQIIMHGSFVD
                     VYGVGLLFSGKSGIGKSEIALDLVERGHRLVADDVVVVSKKGEGVLMGSGMAMSKHIM
                     EIRGLGIIDVSTIFGIRSIRYQKRVEVIVELEDWDTDKAYDRTGLDNTTVDLLSVDIE
                     LIKLPIFPGKNITVIAEVIALNYLCKHYGYNAAEVFRKKLQEKISRKSDKDSLYSRDD
                     RLEKATEYFEHDFE"
     gene            complement(30755..31090)
                     /gene="raiA"
                     /locus_tag="PLX80_00700"
     CDS             complement(30755..31090)
                     /gene="raiA"
                     /locus_tag="PLX80_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQY19343.1"
                     /translation="MIKVNITSRHFKAHDTLQDYIRSEIDNLSKYNEDIVYADVILFF
                     EKAINSIKYCEILVKIGSKDLMAKESSDDFVKSVDKAIDKIETQILKIKDKNKTDKHN
                     TKKEITKTI"
     gene            complement(31263..33062)
                     /locus_tag="PLX80_00705"
     CDS             complement(31263..33062)
                     /locus_tag="PLX80_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY19344.1"
                     /translation="MKKINLVVFFIFSSVLVISCGRNKETSQNSNTESFPITELQPID
                     TANSDYGDWIIKHEMSDAEKLNPIVTNDATATGIFIYMFENLLDLDRVTYELKPLIAK
                     SMPEISEDHLSYTYDLKENVTFSDGKPLTAEDIIFTVKVIKNPFTDAQASRNYFMDVK
                     SVELVDGNKFKVRFNMSKPYFRAIYSISDMEIIPKHILDKNNETDKFTWEMLEEAGKT
                     FDPVKYPGLQKYADFINKEEVSRSPEYVIGSGPYKLEKWVTGQSITLVRNENYWNKSE
                     LPNYPQRLIFKTIKDQNAAVVAAKNKEVDQLQVIQPIDFVENIKDPEKFQLKKALVIE
                     PSYNYIAWNNESPIFSDRKVRWAMAYAIDRKSIVDKIVYGMGTNIESHIFFKSKYMNT
                     DLPPIPYDIGKAKDLLAEAGWKDTDGDGTLDKMIDGNKVDFKFTFTNNTNPKRKKVIL
                     ILIEQLKQLGINADIQEYEWSVFLDKIKKHNYDACFAGWQLSVTADDPYQIWHSSQSI
                     GEGSNYFSYKNPEADKLLEENRLTFDDAKRKEILDKWQLLVYEDQPVTFLWSEPVRYV
                     YSDRYKNARWYAYPDSPLLNEWWVPANRQLYKN"
     gene            33151..33687
                     /locus_tag="PLX80_00710"
     CDS             33151..33687
                     /locus_tag="PLX80_00710"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HQY19345.1"
                     /translation="MKNKSGDNELNSNDDSISKKVYYIILIFSFLWLLLIFLAPVLKD
                     IGGVFADISSYLYMFFSPVCHQDDARSFHIFSEKVAVCSRCLWIYSGFTAGTVLYPLK
                     YKLTNENSPSVIFIIAASALIALDVLLDGQEIIKNSFISRSVTGFIIGLTLPFYLIPG
                     SVRFFSEVNLFLKNKSAG"
     gene            33693..34193
                     /locus_tag="PLX80_00715"
     CDS             33693..34193
                     /locus_tag="PLX80_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19346.1"
                     /translation="MQSNNKLTPVIIGTLVMTFIALFPIVNFLNILCCSGIMIGGFAG
                     VTSYNKQLTNSGQTLTAKDGGMIGILSGILSAVFVSGFGLLFSLFSNQNPMTEIMNAF
                     SEFGFQIPDSAAYYLDKFSNEFNDHGFSPTLTIFSFITNLIIYPLFGTIGALIAVQIY
                     KKKGNV"
     gene            34195..34629
                     /locus_tag="PLX80_00720"
     CDS             34195..34629
                     /locus_tag="PLX80_00720"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HQY19347.1"
                     /translation="MGFISIVKTGPSESRKIRIYLIIILFFVFLTSYFSPAKLIRQFI
                     PDLFNRESSCILLNVSGIPCPLCGFTRSFEEILKLNIAGSFYYNPSSVFIFTFLACLT
                     LFIFVISFFNYKIKIVSADKTFLALTVVLVTVWIVNIFFGHH"
     gene            34695..35780
                     /locus_tag="PLX80_00725"
     CDS             34695..35780
                     /locus_tag="PLX80_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQY19348.1"
                     /translation="MFEYTGSLHMHSVFSDGSGTVEDITKAAVSSDLDFIILTDHNTL
                     KAKDKGYEKWYKKTLLIVGYEVNDRKNKNHYVVLGTDKITGTYEKLPDGDTGCRMTAS
                     EYVKAVNDEGGFGFIAHPHEKRQNLPEHPPYPWTDWDCKDFNGIEIWNHMSEWAEDLN
                     ESNKFQRFLHPLKSIIAPQQKTLKLWDDLNKKRKVVAMGGIDAHAHKHNVMGMEFEIF
                     PYKVLFKSIRTHVLLNEELKKGNQTSYIESRDKILDALAYGRSFVVNNYYGSGKGFRF
                     YAEYDGAFYTMGDEIKFDRTKNKKIIFRALLPSKAKIKFIQNGKCTDELEGMECIWDR
                     DEPGIFRVEAWMENRGWIFSNHIRATV"
     gene            35936..37462
                     /gene="purF"
                     /locus_tag="PLX80_00730"
     CDS             35936..37462
                     /gene="purF"
                     /locus_tag="PLX80_00730"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQY19349.1"
                     /translation="MKIKKFKNTGFNSTKSIHSNCGVFGVYNNPDASVLTYYGLHALQ
                     HRGQEAAGIVSCEQNQNGDKSKFNIHKDLGLVLSVFSDDKILTDDLKGTSAIGHTRYS
                     TTGSGDNRNNIQPFKVNYKDGNLALSHNGNLTNTKFLRNKLQNEGTIFQTTTDSELIL
                     HLIAKSKESDVVNKLLDAFSQVVGAYSICILTDDKLFAIRDPHGVRPFCFGKVDDSYV
                     FSSETCALDIINADYIREVNPGEIIMIDREVVETGIEKSFQASTVDKYKHCIFEYIYF
                     SRPDSTVFGEKVDKVRRTIGKNLAIESPAPVREPEDTKKIGIINVPDSSNTATLGYFN
                     EMVKHNKDVKHEIGLIRSHYVGRTFIQPGKDKRKMKVKTKFNTVKGVIKDRKLVVVDD
                     SIVRGTTSKLLVDLIKKADPKEIHLKITSPPIVSPCYYGMDFPTAEELIANQHDSNVE
                     GIREELGVTSLSYLSTEKLLESVPYSNPKTDFCTACFTKNYPIQIEGLEEMKKEQFDD
                     "
     gene            37514..38842
                     /locus_tag="PLX80_00735"
     CDS             37514..38842
                     /locus_tag="PLX80_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HQY19350.1"
                     /translation="MNKDQLNFHKSRRIEFSKLIGNDAIALIFGNTHRNKSYDGDYKF
                     KQYKNFYYLTGFTEANTALVIAPGGLPIEADKKKLTAKEMLFVQQKDERLETWNGKRL
                     GYKNVKKSLGIEHSDININLGKILNSRYLGKYRKFYVNFAELMKLTGEMKKIVSDFME
                     SLNIIAPNVEIIDVSYILGKMRSIKSEFEIERIRDASHVSVGSYNEILEILEPGMNEF
                     EVQSTLEYYYRYHGGDDIAYHPIVAAGENACILHYENNDQTLKKDQLLLIDSASEFNY
                     YCSDITRTFPVSGKFTDEQKQIYEIVLKANKDCIKNIKPGVKFSKIAEMSDKILAEGL
                     HKAGLLKDKSKIKKYSLHGIGHHIGLDTHDAVPSSLTKTSDTDTLKPGMVLTIEPGIY
                     FTNDMKEIPAKYRGMGIRIEDVILVTAKGCDNLTEAMVKEVKDVEERMTS"
     gene            complement(39069..39767)
                     /locus_tag="PLX80_00740"
     CDS             complement(39069..39767)
                     /locus_tag="PLX80_00740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY19351.1"
                     /translation="MKKTVVITGASKGIGLSCAEIFLKNNYTVINASRTNAKSIKDPD
                     FHFIKTDVSKEADIKKLFQAVIKKFKKIDVLICNSGFGRFANLADTTTKDFDEMFSVN
                     VKGLYLCNRFGVKEMIKKKSGTIINISSIGGKNGVATASIYSATKHAVMGLSRSLMAE
                     VRKDNIRVVTICPGSVDTNFFDHPSAGLTANRETLLSPDDVAETCLLAVNLPDRALIN
                     EIEIRPLNQRIKQR"
     gene            40057..42294
                     /locus_tag="PLX80_00745"
     CDS             40057..42294
                     /locus_tag="PLX80_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQY19352.1"
                     /translation="MYLNLNILNPEQRKAVEEIEGPSMIIAGAGSGKTRVLTFKIAYL
                     LESGVSPFEILSLTFTNKAAAEMKDRISKLVATSFEKIWMGTFHSIFARLLRIEADYL
                     GFNRNFSIYDTDDSASLIKQIMINLSQPTDNPTPKAVHNFISNLKNKMILPKEFSTMA
                     KSTFEKKIEIIYEEYQKALFKNNAMDFDDLLLKPIELFRRYPEILEKYQERFKFILVD
                     EYQDTNKAQYTIIKLLSDKYKNLSIVGDDAQSIYKWRGAEIQNIFDFQDDFKECKIFK
                     LEQNYRSSQNILTLAGNVIKNNKKQMEKELWTENDEGEKIHIIESMTDRDEAVRICRN
                     IYDEIQRKKINFKDFVILYRTNVQSRTIEDALRNNNIPYLIIGGVRFYQRKEIKDILA
                     FLKIIVNPQDNESLTRVLGLRAGIGKTTIDKLIAKAENEGIQIFELLKKISAEKDLSS
                     KVKNLLIEITNYVYKFQYLQEEVPLTELVNSVVDEIGILRALRLENTVESEERIGNIQ
                     ELLSAVAEFRDTDDEPTLEMFLQKVSLVADIDNLDNQKNAVTLMTIHSAKGLEFPVVF
                     ITGLEEGLFPVSGANMYEDELEEERRLFYVAITRAETKLYISFANQRYRFGTPSFQMK
                     SRFLKEIPEDIYNSIITFENLKLKTGYRSGGEKKSVSGSAPSVKFNPTPKKKNSSLNV
                     EDEFNDKFPDIKKGVTVTHNTFGKGTVLNTVGKGIDKKAEIYFDDVGLKKIMLKYAKM
                     TVEDS"
     gene            42332..42742
                     /locus_tag="PLX80_00750"
     CDS             42332..42742
                     /locus_tag="PLX80_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQY19353.1"
                     /translation="MNYWLLKTEPDNYSYDDLVKDKKTVWDGVANNAALICIRNTRKG
                     DKAFIYHTGAERQIVGIAEIVSDPYPDPKADNPKLAVFDIKPLKKLNTPVTLAQIKAI
                     KKYLEFPLVKEGRKSVVEVPKEYWDDFLKMSGSK"
     gene            42850..43320
                     /locus_tag="PLX80_00755"
     CDS             42850..43320
                     /locus_tag="PLX80_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQY19354.1"
                     /translation="MKKIIFTIFFLSIIFSSEYSYSQLTKATIGVDGFTCSLCAKGVQ
                     GQFESLDYVKSVKADLKNTLFILTFKSKPAINIKELNSAVTDGGFSVRNISVVAKGTL
                     KGNNTSGFWLSAPNIPDIKLNDIDESFTDGDKISIEGVVNIKDLSISVTSISKL"
     gene            43503..45101
                     /locus_tag="PLX80_00760"
     CDS             43503..45101
                     /locus_tag="PLX80_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19355.1"
                     /translation="MDHQMSGNTKVKKDKNPAVIIPGLGNYHYKVTTDSKKAQQFFDQ
                     GLILVYGFNHHEAVRSFKMAAKLDPDMAMAYWGIAYALGPNINLPADSLQRKQAYNAV
                     RKALKLSVNSSKKEKDFIYALSKRYAEDVNKTEQSVKDKEFSYAMKDVMTKYPGDLDA
                     AAIYAESLMNLKPWQYWNADGTPADGTEEIILVLENVIKKDPKHPGANHYYIHAVEAS
                     DDPGRALASAELLKDLVPSAGHLVHMPAHIFFRVGDYEGASIANINAINSDEDYIKKY
                     KPEGIYPVMYYNHNINFLSVSRMMEGNYIEAISAANKIADNVKPYLKDMPMLEGFYAN
                     PLLINISFAKWDDIINTQVPDTSMNLYSAIYYYSKALAFASNDNQDQTRYCLNEFLNF
                     KSKITEDVIIGLTPAALILNMMQDIIFANVALNEDRIDNAIKLFKDAVAKEDKINYDE
                     PPDYYPSVRLMLGAALYQNEEFEEAEKVFRDELKKYPHNGRGLFGLKESLLAQEKITE
                     AEEVDKEFIKAWINADVILSMTDL"
     gene            45108..45554
                     /locus_tag="PLX80_00765"
     CDS             45108..45554
                     /locus_tag="PLX80_00765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19356.1"
                     /translation="MNVEIKKITAAGTRPLRQIILRPHQNADELVYEDDENPDSIHLG
                     AYINNELCGIASLYKHRLKDNAEEEAWRLRGMATSESCRGKGLGVILMNECIKYVEEK
                     NGLIFWCNARTTAEGFYEKFGMKRHGGVFTPEGLGEHVVMLMNIKK"
     gene            45561..46412
                     /locus_tag="PLX80_00770"
     CDS             45561..46412
                     /locus_tag="PLX80_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY19357.1"
                     /translation="MSDIKKLSLKIKNVHGDELNTDIRFPADLKENKKLPVVILCHGF
                     KGFKDWGCFPYMMERISNEGFCTVAFNYSYNGTGENEDEQSDFTRLDLFAKNTFSREL
                     DDTGSVIDYLYANSDELKIDTENITILGHSRGGGIAILKTAEDERINKLVVLSSVCSF
                     DRYSEMLKSKWEEAGFFEVLNTRTNQIMRMDYTLIEDLEKNKDRLDIQNAVSKIGVPA
                     LIIHGTQDITVDYSNAEDLYSRSNKDLFELKLIENTGHTFGAVHPFEGSTPALEEVIL
                     KVIEFIK"
     gene            46429..48006
                     /locus_tag="PLX80_00775"
     CDS             46429..48006
                     /locus_tag="PLX80_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY19358.1"
                     /translation="MKLKKLILPLLPFVLIILFSINAFSYNESESSDINTTDRTELAA
                     EYKDEKPLTEKADETEHKESESHGDKFAEIYLILFLIIIGAVIGRRFLAKKFNQPEVL
                     GELMLGVLLGLILYQLNNPVMFLLRHQDKVNEIVQSGVSGNITWDEAINKNLTIEDYE
                     KGGIGNELSEILTSPTFARDNLFVQIILLFSGFGVLILLFLVGLETSFEEMFEVGGSA
                     LVAALIGVLAPLILGYISTMLLFPDGGTNLYLFIGATLSATSIGITARVFKDMKRMHV
                     PEAKLVLGAAVIDDVLGLIILAVVAGIIESGEVQIGNIFLITLKALIFLSLTFFIGKQ
                     YLRKQIKLASLVQGKNISILFPFAMMLLMAWISDSIGLASIVGAFAAGLIIKEEYFND
                     IPGDDAKSVKSVIEPIEAIFAPVFFVIMGMQVDLSVFLDVNILGVSLLLTIAAVIGKA
                     LCGIFAKGMDKKIIGIGMIPRGEVGLIFASIGKSIGVLDNAMFSSVIIVVILTTFITP
                     PALKWAFSKYDKKSIAA"
     gene            48028..50586
                     /locus_tag="PLX80_00780"
     CDS             48028..50586
                     /locus_tag="PLX80_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19359.1"
                     /translation="MRKFFYIIFFTVFVLTGIYLLYNENNSSVETTYAENSYLGISEF
                     TDKFIPSDDWMEKQRAFPFDEIPHEEYLKSVEYVKKNMRSEFPGNTTGWNLAGPTNIE
                     GRITTIAIHPTNPQIVYTGTANGGLWKSTDFCQSWVSVFDGQNTSSIGAVAIDPVNPD
                     IIYCGTGESNSLRSYYPGTGVFKSTDGGSSWNFIGLDSSFSIGNISVNPQNTQEVYAA
                     AGGWTRRKNEQRGIYKSINGGQSWQRSLYLSDSVGAIDVSVDPSNPSKVYAAMWERQR
                     REDYIKYGGRFSGMYVTANSGANWQQVQNGFPFNDNNLGRISFDISASDPNYIYALTS
                     NSNGFSRGLYKTTNGGTLWTLINSSVAGSSNYAWFNRICKVDPSNPDKLFCGGLSMEL
                     SINGGASFGFPPTSHVDQHAAAFSVSNPNYIVVGNDGGIDYSTDGGSSWSESTTLPIT
                     QFYAGEINFNNPDDILGGTQDNGTIRATSNFENWNLIYGGDGFYCLVDYNDPQKVYAS
                     SQSGGLGRSVNGGNSFTSATGGLDLQYTNWMTPYVMDKNDPQTLYCGTYKIHKTTNGM
                     QSWTAISPDLTNGHTANLGTITTVDAAKSDPNVIYCGTDDANVWRTTDAGSNWTKINS
                     GLPYRWITRVAVHPDSANVCYVTLSGYKVDSTGSHIFRTTNFGDTWNSISGNLPDAPV
                     NDVIIDPEISNTLYIATDINVMYSTNLGAHWNILSAGIPSNVPCHDLTFHAPSRTLVV
                     WTHGRSAYKIILPVTGISQNQSSIPSAFKLNQNYPNPFNPKTVINYELRVTGNAKLLV
                     FDVLGNEIAVLVNEKQGAGKYSVEFDGSNYSSGVYFYRLSAGEFIETKQMVLIK"
     gene            50711..51448
                     /locus_tag="PLX80_00785"
     CDS             50711..51448
                     /locus_tag="PLX80_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19360.1"
                     /translation="MLKTILTCLAAGILITSCKDSENNTKNISIENQNPVASVEVYGQ
                     RYCEIFVVKGNVTDLEAAVYNTLGCNDCPQEVWSVIDPEKLAKELDAKKIIMNGPRVF
                     LMNKIGQFNEPPPKEKIGGLEMVKRAVLPLKIKTLLAGESEPYVENKVNRQTQYIFNK
                     GTEVYLLINGDIHYIMQSFAMITDPDLKIEDLKTLNSKIKLPSGWKYEVKVLDEEFVL
                     ETKEGGEAFVIQDDLKNTYQKINNGKL"
     gene            51458..52654
                     /locus_tag="PLX80_00790"
     CDS             51458..52654
                     /locus_tag="PLX80_00790"
                     /inference="COORDINATES: protein motif:HMM:NF041516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2928 family protein"
                     /protein_id="HQY19361.1"
                     /translation="MKKKLSVYFILFLSLIFISCNPMKKNVMKDSCDDMKEVVISESN
                     GKFALVVKEEIFQATSKSDNGGIRNITGYTEFRLSSYDLNTGELLKRLELGEIRENEC
                     ILLGMTKSKLWYKSIDSELGFHARDPVTLDIIVTEKMVTDANPFLQNNISKPEWNSIQ
                     KFYGFDNSIGMPVISDNSGYLYSIDPVTLKAEKITSSIMNIRYDENVLTSSVYLNNNE
                     VVYLSGNPRTRFKYVNKDFDEPSFLKGEIIKSSTETEMPEEYLLSNEFKESVSKKNPL
                     RISRKTEPEYSFAKGQIITNDKSIFILSRTDVTDQAKVLISKVKFDENYSPSLQWETV
                     LDNIFRDPDKALDQSGFEMVFSKGNPDLSTMRVAYADNKLIFIFMLKAVCLDINSGKI
                     LWSRDL"
BASE COUNT        17354 a         8940 c         8723 g        17759 t
ORIGIN      
        1 aactaacttt aaagtgtttg tgacggatta aatcatgata aaatttgcag ttaagcctgt
       61 tttacttcca aaacattcat ttattgcatt ttattgacag aataaaaatt ttaaattgtg
      121 aaaacttctt ttggatgata aactacaaag cgatcaatat tttgaaaggt ttgacaaaaa
      181 aagaaatatt aagattcgaa aaatttttgc tctcaccttt ttataacaat aacaaaaaac
      241 tgcttttact atacaattac ctgaggaaat tctatcctga atttgactct tcatcattta
      301 ctaaggaaaa tatttacaga cacgtttttg gaaatgatat attcagtgat gagaatctga
      361 gaaaactatt ttcagatttt tataagctgg gagaaaaata tcttgtaacc ctgaatctcg
      421 aaaaaaataa gtttgattat gacagatact tacttgatga gcttgactgg agaaaaattg
      481 acaatatttt cctttctaaa ttcaaagaaa aagaaaagca gtttgaaaaa tccgaattta
      541 attatcagac cttttactat gattatttaa tgaaatggca gatcgtatca tttcatcttg
      601 acagaggtga gcaatacaaa attacgggtg atgtgtttga aagaactgag caactgattt
      661 tttatttttt atcagatctg tttataagtc tcaacgatat agatgtaaac aaaaatactt
      721 tcaattataa ccccaaaatt aacctgccgg aaaaatttat agaactgctt gatcttgaat
      781 ctctttatca atatatcaga gataataatt tcaaaaataa agatttgttt tcaatgtatt
      841 ataatctgtt tctgatgaat aaaaacagaa ccgatgagtc atattttttt gaactgaaag
      901 atcaattaga aaagaatttt gaaagattaa gcatcaggag taaaatgcat ttcattttga
      961 gaatgatctc aatgtgcaac agatatataa atctgaataa gtcaactaaa tttcatgaaa
     1021 tcaaacttga tctttatgac aaaatactga aatacaaact atataagaca aaccagaatt
     1081 atttcagatc agatctgttt ttaaatttgt ttattgaata catcagctat aagaacacat
     1141 ctgatgcgga attatttttc aaagaaaaca taaaatcaat tagtcctgca cacatttaca
     1201 aacttcaatc gttatgcagt gcattattac tgtttgaaga aggtaagttt ttggagtcac
     1261 ttgagatctg ttccagaatt aacacaaatt tgctgacagt aaaaattatt ttaaggaaac
     1321 ttcttttgaa gatcttttat gaagtaaaag attatgacgg caataaggat gagataaaga
     1381 atttcagaca gttcatatca aacagtaaac ttctgaatga aactcacaaa agttctcttc
     1441 agaattttat aaaattattt aatgatctgg tgaatttaaa aaatgatccg gaggacgaat
     1501 ttttaaatta tactttgaag agtaatacgg aacacattat tgatacttca gacagagatt
     1561 ggttcagaaa taaaatatcc gaattaaatt agaattaatt cataaaatta attcatgtaa
     1621 aatttttaat tactacatta aaaacatttc aacacttcac ataaatattt agtctgagat
     1681 cttctggaat attttttata cagatcattt tctatattca gatcaccttt gactttccag
     1741 tattgtacta atccagaaag atattccgac agaccatatt catcatttct ctcaaaagtt
     1801 tttcccgctt tgcattcact gataaaagcg gcagcttcgc cgtcttcagg accaatgcat
     1861 acaatggttt ttcctgatgc aagatattcg aacagtttac cggtaaggat tcccttgtca
     1921 ttttttactt cgggaataac cagcagcagc aatgtgcttt cgttcaaata ttcgactgat
     1981 ctttcatgag agactgtagg gatcacttcg agattactgc tcattgacat ttcacgaatc
     2041 ctattcatca tagaatctgc cggactccca atgaatctta actttatatt tatttcagga
     2101 ttttcggtta ccactttttt caattcatca aagaatatct gcggattata actttctgca
     2161 agtgtacctg tgtatgagat aatgaaatca ttttgagtct tgtctggctt ttttaaattc
     2221 ttaaaatcat cttcatcaaa accgtttgga atgattgtga attttttttc atcaatacta
     2281 tcagactttt gcaagaacat tcttttgagg tcattgctta cggtaattat tttatcagca
     2341 ttttccagaa caagccgttc atatttttta tcaagtttct cagcaaacgg aagatgtttg
     2401 aattccttat aataataaat atccgtccac ggatcccgga gatccgcaat ccaatttatt
     2461 ttatattttt ttttgagttt aagcccgaca agctgtgcgg aatgaggcgg gctggtagta
     2521 attacaatgt caatcttttc tttctcgata agtttacaag cttctgtgaa agcaaatttt
     2581 atccagcctt tccttgcgtc gggaatgaag aaatttcctc tgataaatct ggatagtttc
     2641 tgtatcattc cggggtcggt ttcatttgaa aatccggtcg ggatactttt cttcccgatt
     2701 agtttagaat aaatatcgaa aggttcaaac gtgccggttc ttatcacttc ggcttcgtgt
     2761 ggcacttcgg cattcagcga cttatcaata acaggataac tcgccttttc ttcgtccacc
     2821 gtcacaacaa acggttgaaa accgttttcc ggaaaatact ttacaaactt aagaatcctc
     2881 tgcacacctg caccaccgcc cggcggccag taatatgaaa tgacaagtat ttttttgagg
     2941 ttcaaagttt ttgcagatat atatttatat tctgttttat tattatttgg agaaacggtt
     3001 ttaaccgttt ctgtaaagta tatactgttg aaattgtctg gaactcagga tctactgaca
     3061 aaacggtttt aaccgtttct gtaaagtata tacttttgaa aatgtccgga actcaggatc
     3121 ttcagacaaa acggttttaa ccgttactcc acagcacatg caattacctc atcattagat
     3181 aaaactccac tgtccagact gcaatcaaaa aaagtgaaaa tcctgcagcc aatatttttt
     3241 tatttgtaga aatttcattc aaagatcccc tgcctaaaga aagaaacatt accgtaccca
     3301 gtataaaaaa tggaatcagt acgtaatgat atcttgcaag tgctataaat acaacaactg
     3361 tgaatatcca aatcagtaca gaaccatata tgaaaaactt tttccttcca aaatacataa
     3421 gcccccatgt accgagcagc ataataataa aataaggaat attaacaagc aatgtaaaca
     3481 acgggcttgc tgatctgtaa acatctgtag atgtctgtcc gggaagtgtt tctgtaaaat
     3541 aatttactat catccgctca ctggaatata aatagccaat cttttttatt cccaaaatca
     3601 tgtaatttac cggatgtgaa actgcataat caagtccgag cttaaaatat tctttgtctc
     3661 ggtcagcttc attactataa tcaccaggca gcatatcatc aggtttaata aaataagaac
     3721 cggtagcatc cggattactg cctaaccaga aatttaaacc tgatgtagtt cccaggctgt
     3781 acgttccgac catcatttta tttctgatac tccatggtgc aatcactaaa atcattccca
     3841 taaaaaagat cattgaaaat attaccgctg atcttacttt agaagttttg aatatctctt
     3901 ctctatatga ataaaaaaaa agaggtacta atatacaaat aggtaaataa gcagtgcgga
     3961 tcaatactgc taatccaaaa attagacctg ctacaaatat ctgaattggt tttctggaaa
     4021 agtcatctct taaaaataaa tacagcactg ccattgaaaa aaatccaaac agaggttcag
     4081 ataagatagt ctgactgtat aaaatatttg acgggaaaaa tgaaaatatt gcaactgaaa
     4141 taagtgaatg ctttatacta aagaatcttc tgcttactcc gtaaaaaagc aatgccgttc
     4201 cagcttcaat cactgactga acaagcctta cataaaactg accttccccg aaaataaaaa
     4261 atattcccga aagaaaagct gggtatccaa tacctacata agcagttggt ttcccattca
     4321 acgaatattc tccgttaagt aagctgtttg ccagagtttg ataatctaga ctgtctgaac
     4381 gtaagacagg atcgagaaga aacagtaaaa ttaattttat taaaaatgca caggataata
     4441 taaagatcaa aacagatctt tccgttttca aaggagtgtc agaaatttca ttcatttatg
     4501 ctaaggaata tttggagaat ctgttccgga aattttatca gtggaaagat taattttcat
     4561 cctatcagct attaataaag agtctgtatc cccaatctct gtaagtttat ctataacaat
     4621 tttttgtctc tgcatagaat ggttttgtga caacttttct ttaccctcga cagaagatat
     4681 tgcaatccta aatgcaacta ttcctttcaa aagattttca agatattcag gtttaagatt
     4741 atccagagaa taaatatctt tatccggttc gaaaagatct acaagctctt taataatttc
     4801 aaatttttca tccctgtcaa taattacctg aagttcacca taaacatgca cggacatata
     4861 attccatgta ggcactgtct gatctgattc ataccaaccg gatgaaatat atttatgcgc
     4921 tcccggaaaa atgacgagta cttcccctga aatattttga tgctgaggat ttgcttttgc
     4981 aatgtgacaa tacaattcac catacttttc ttttcctttt tttaataaaa aaggaatatg
     5041 agtagcagta atttttgaat cattcagagt tacgagtatt ccgaaattat ttgattcaat
     5101 aaaatcaaaa atctcatctt catttttcat tttggtatgt ttcgggatat acatctttat
     5161 tatttacaga agtttatatt ttttttgaaa ccattgtaat gcccgtacct tctttattat
     5221 atgtattgac atcaagccac ataaaaatca gcacaaacca tagcgtgaag atatagtaca
     5281 aaggcagtag cagtataaga aattttgaaa caccggcaat aagtattgga tatttgattc
     5341 cccatctcca tgaaatagtt ccgtattttc cgtatgtata atcaaacgaa tgaacttcca
     5401 taccggctcg gtttactttt tcagaaatgt cttctttaga atatccgatc cttgcatgtt
     5461 cttctataaa actttcatca tcctcttcat gtgcatcgct gccgccaagg tttgacggag
     5521 tattgaccaa aagcatcccg ccttttttca atgctcttga aaaattttta aatacaagtt
     5581 catcctcgac aatatgctcc attacatcga cacataatat gaaatcaaac tgcctattat
     5641 aatcaatttt agtaagatca ccaacttcaa atcttacatt cttaaatccg cattttgaaa
     5701 aaaacctttt gcagtcatct acctgatcct cttttacatc tattgcaaga atatccgaat
     5761 ccggaaaacg ctttgccatg aaataagtat actgtccgaa tcccatacct gcatcgaaaa
     5821 tattcataac tttacctttg ggataaagct cacggacttt ttttcttaca tgccatgacc
     5881 tgagaaacat tagatcaaga agccagtaaa atatttttct aaaaaaggag ttatccgaaa
     5941 ctatgtcacc aaatactttt ttaaccggat cgtatttcaa attaaattat ttttttaagt
     6001 ttaaattttc ttcaatgtaa attcaaagtt ctattttcca tataactctt ctattgcttc
     6061 agtcattctc ccccattcat actttttctt ttcatctttt gcgttttcac tgaacttatt
     6121 ttcaagatct tctttgtaaa atttaattac tgcttttgca accgactttg ggtctttggg
     6181 ctttatcaga aaaccggttt caccatcatg aactgcttcg gacaaaccac cgacatctgt
     6241 agcaattaca ggcttatcat aataatatgc aatttgtgta attccgctct gagttgcatc
     6301 tatatatgga agcacgacta catccgatga acagaaaaaa tatcttacct tttcatttgg
     6361 tatgtaatcc gatactacat gaatattttt ttcaagtcca agactattaa tatgatcatg
     6421 agttatcttt tcatcttcat aaaattcacc aacgattagt aaagtaacat ctatggattt
     6481 taatatttca ggcattgcat ctatcagata tttcaaacct ttatattttc taatataacc
     6541 aaaaaagaga atgacttttt taccaatcaa atcaatatta aaatctttat taatattctt
     6601 aaccgcttct tctttactaa tcggatctcc aaaaatatta tatatcggat gcggagtata
     6661 tttatatggt ttacctttta taaatttctt caggtcttcc tctacacttt tagaatgaat
     6721 aataaaataa tctccgactt tgaagataag ttttataaga aaattatcac cgggtctttt
     6781 ttcgtgtgga ataacattat cacaaataaa aagtatttta gcaccggttt ttttcttaac
     6841 gtaactacat atcctgtaaa aagccggtgc gaaaaatggt atccagaatc tgagaattat
     6901 aagattaggt tcttttgcag ctatcatctt cgctgtcttc tgccagttca atggattaat
     6961 cgagtctatg atatttgtat ttggaatatc cttattcaga atatcattat cgctcgtatc
     7021 catctgtgtc ttaccgggaa aaagtaattc aggatattgt cttttaaatg aaaatatctc
     7081 aacgtcatga tcttttgaaa gataatttgc aagtatcgca ttaagctgtg ccatagcacc
     7141 tctcaacgga aatgcagttc cgacgataat tattttcatt ttgataaaaa ttttagcaaa
     7201 aataatttaa tttgatttta ataactaagc aaaaaaagga gaaatttaat actttggtaa
     7261 catttagaaa gagattcttt aaaattaaat tattattttg aattcatatt atggcagaaa
     7321 ataatttaga aaaacttatc agcaaaaaac tttcccgaag agaaatgttg aagacttccg
     7381 gaaaatcagc agcggtgata gctttgactt ccatttttcc tgtggggttt ttaaatctct
     7441 taagtaaaaa tgctaatagt ttaacaactg atttctctgt tccgtttatt ccaatttttc
     7501 catcttcaga ggacaaacta tttttgccca aagaatttga atataccata atcagaaaat
     7561 ggggtgatga aattgctgac ggagtacctt atggatttaa taatgacttt tccggatatg
     7621 taccgataga ttttctctct ggagggaata attctgagga cggacttcta atagtaaatc
     7681 aggaatatcc atcacctctt tttataaata attattcaga tgaagatttc agaaacggaa
     7741 ggattaaaac atcagaagaa gtaaacaaag aaaaattaag tacgggtata aatatttttc
     7801 acattaaacg aattgacgga aaatggaaat tcgtaaacga taatagatac aacagaagca
     7861 taaacggaac gacaattata aaactttcag gtaaaactgc aggatctgaa gaaatgaaat
     7921 tcaaagaatt tgcttccggt actttggcaa actgcagcgg tggtattact ccatggggaa
     7981 caatgctcag cggtgaagaa aattttcagg atcattatgc atcggacaat atctgggaat
     8041 acagatggaa tgatgcagat aaggatttta taattgagca ttacggatgg gtagtggaaa
     8101 tcgatccttt tgatattaat tctgtaccga aaaagagaac ttctctcggc agattcagac
     8161 atgaaaatgt tactgtcggt atttcatctt caggaaaagc agtcgcttat atgggcgatg
     8221 ataaagtaaa tgaatgtgta tataaattta taagcaaaaa taattttaac ccagataaga
     8281 gatctgcaaa tatggatatt ttagatgaag gagatctata cgttgctgat tttttaaaaa
     8341 ataaatggca gcttcttgat tttgaaaaaa gggacgattt aaaaagcatt ttcaaatctc
     8401 aggcagatgt gcttgtaaac tgtgataaag cagcaaagac agtcggaggg acagaatgca
     8461 acagacccga agatattgaa ataaatccga aggacagatc tgttttcatt tcatttacaa
     8521 acaattcaaa gaaagaagat tattttggaa gtatagtaag gattatggaa aaagataatg
     8581 atccggagag cctggatttt gaatgggaag tctttgccaa aggtggcgca gacacaggat
     8641 tttcttgccc tgacaatctt actttcgata atgacggaaa tctatgggtg ctgtgtgata
     8701 tttctacaag taaaataaat aaggaacctt attctgaatt caaaaataat tcacttttta
     8761 tgataccggt ttcaggaatt gataaaggaa aagctttttt attcgctaca ggtcctgttg
     8821 attgtgaaat gtgcgggggg acattcacac ctgatggatc aacaatgttt ctttctattc
     8881 agcatccggg agaaggaagt ttatattatg atcagccgac aagccgctgg ccagagtatg
     8941 gagatgatat tccaagacct gcagttgttg caattacagg ttttaaaaat tgagttattt
     9001 aattatattg attatacttt ttttaactta ttgatattta aatggatgaa gtatataaat
     9061 tttttgaaac taataaaaat gcctggaata aaaatactga tgtgcatatt acatcggaat
     9121 tttatgacaa tgaaaatttt ataaatggta agaattctct gaagcatatt gaaattgaaa
     9181 tgttaggaga tattaagggg aaaagtattt tacatttgca atgtcatttc ggccaagaca
     9241 caatctcact tgcaagagca ggagctgttg taaccggaat agatttttca gataatgcga
     9301 tcaataaagc aaaggagctg gtagtgaaaa ctaagactga tgaaagcact gaatttatct
     9361 gttctgattt atatgatctg aaaaataatt tagataaaaa atttgatgtt gtatttacca
     9421 gttatggagt cattggctgg cttccagatc ttaaaaaatg gtcggaaata atttcacatt
     9481 ttctgaaacc gggcggtaaa tttgtaatgg tagaatttca tcagatgctc tggatgtttg
     9541 atgatgattt taattatttt aaatatgatt attttaatag aggtgaagta aaagaaacaa
     9601 gcgaaggcag ctatgcagac agaaacgctc ccataaaaag cgattacgta atgtggaatc
     9661 acagcttaag tgacgtaata aataatctga taaaaagcgg cttggaaata aattcttttg
     9721 aggaatatga ttattcggtt tatgattgtt ttaataaagc agtagaatat gaacagggca
     9781 ggtacagagt taaaggaatt gatgtttgtt ttccattgat gttttcagta actgccttaa
     9841 aaaaataaag ctaatttaac tttacacgtc accggaaaga ttgtcattaa tgaaacgttt
     9901 attgtttcgc cagtcttttc tgacttttac aaacagttcc agataaactt tcctgccgag
     9961 aaatttctca atctcttgtc ttgatttatt tccgagattt ttcaaaccgc tgccgccttt
    10021 tccaataata atgatcttct gagtctccct ttcgactacg atatcggcgt ttatataaac
    10081 aagatcttcg gatctttctt tgaattcggt tacattgaca agtatgctgt agggaatttc
    10141 ttcatgataa tacctgagaa ttttttcacg aatgatctct gatacaaaaa atttctctga
    10201 tttatttgta agtgtctctt ttccgaaata aaattcactc tcaggaagat attcaagtat
    10261 tacttttttc aatgaatcga gattatcatc attcaatgcg gatattggca ctacatcatc
    10321 atatccaaaa ttttttgaaa tggtctcgat cattggcagg agttttcctt tatccatcag
    10381 atcgatttta ttcaatacta taattctttt tttatctttc agaatttcaa tgaactcttc
    10441 atcagttttt tttaatcttt ctaaatttaa ttttgcagca tctacgatat gtataattac
    10501 gtctgcttca tcgagtgaag ttttgatctc tcttaacata aatttctgaa gttcatattt
    10561 cggctcgagt gtaccgggag tatcaatgaa aatgatctga taatcttctt cagtcagtat
    10621 tccaagaatt ctatcgcgtg tagtctgaac ttttttgttt acaatggata gattaaattt
    10681 aagcagtgca ttaagtaacg ttgatttccc tgcatttggt gcaccgaata ttgtaacaaa
    10741 gcctgatttt tttaccaaaa tttattttat tatttttatc tgatctgatg aatttaaaat
    10801 tatctggggt ttatttctga ataccgatac gcttccggta atttccacaa gcttatcctt
    10861 aaaactttcg agatcatcga attcactgaa cttacctgaa aatacaacac ctgaaaaatc
    10921 attctttgga aatttattac caaaattcag gtaagctact ttttctgaaa tgtgaacatc
    10981 ggagacaatt cctttaacag ttattatttc tcctaagtga tcttttactt tttcggcagt
    11041 gactgttgat tctgaattac cggaaatatc actttcagta tcttcaccga attcttcatc
    11101 ctcaatttca ttttcagcag tactactact ttcatcagaa aaatttttta tttcaacttt
    11161 atcattttgt ttgtcatttt tattttcatc actacaacca cttataataa tcagacatac
    11221 aattaatgtg aatttaaata aattatttag agcactacaa tagattttta gtcttttcat
    11281 attttccata tttataataa ttaaacatca tcaaatttaa gctaatcaaa agatcattat
    11341 aagcagtcaa aatttttgcc gtaataaata tactatatcg ggaaattaaa ataaagattg
    11401 aatttggatt tgggatagac taacatttct gtttttcaca gagtccgaaa gtatttgact
    11461 tagaggcgtt gacaaatttt ttacagaaaa ttaattttct ttcgatcctg tgtttatcga
    11521 gtttgatgat ctccaaattt taaacttatt gtactatgaa tatcctgtcg catattcgga
    11581 aattggttcg cttcgcccgc aaaatcattc attttttccc gcagaatccg aaaagcacgt
    11641 gtaaataatt atcagttttt aatttgtaat gtacaattta tttgagaatt cttaattttt
    11701 aattctcaat tgtttttccc tttggaatat agtaataaaa aatctctgac ccgaaaaaaa
    11761 aattggatca gagatttgta aaaagaaaaa atatatttta ataattatct atctgtgctc
    11821 tctgaagttt tcctgaatag tcgatgtaga cagctttcca ttctgtataa aaatcataca
    11881 cagtccagcc accttcacga tgtccgttac cggtttgctt cacacctccg aacggcatgt
    11941 gagcttctgc accgatcgtt gcaccgttta cataagtgat accggctttg atatctctta
    12001 ttgctttaaa tgcatcgtta atattttttg tgaagatact tgaagacaat ccgtattttg
    12061 tatcattaag aatattgatc gcatcttcaa gatcattaca ttttatcact gacaataccg
    12121 gaccgaatat ttcttcctgt tcgattctca taccgggttt tacatctgtg aagatagttg
    12181 gtttatagaa ccagccgtct gcaagttcat cttcagttgc aaattcaccg ccacagagaa
    12241 gagttgcttt atcctcttcg agaccgatct ttacataaga gtgaacagaa tcacgctgag
    12301 attcactgat acatggacct atctgattgt ccggatcatt accatagccg agttttaaag
    12361 tcttagcttt ttcagagagc attttcaaaa attcatcatg aatatcttta tgaagaataa
    12421 gtcttgaagt tgctgtacat ctttgccctg tagttccgaa agctccccat aagactgctt
    12481 ccagagcgag ttcgagatca gcatcattca taactatctg agcattcttt ccgccaagtt
    12541 caagtgaaac tcttttaaga tcaccgcctg caacttcatt aattctttta ccaacttttg
    12601 tagatccggt aaatgatatc agatctatat cattatgtga tataattgct tctccgacta
    12661 tacttccttt tccatgaaca atgtttacta cacctgggat ataatctttt ccaagaactt
    12721 ctttaatgga agattcaata atttcgatca aagtcgcagc agtttttgga gtgagttcag
    12781 ccggtttaaa gacacatgta tttccacaga ctagagcggg aaacattttc catgtaggaa
    12841 tagccatcgg aaaattccac ggtgttataa atcctccgac accgatagga actctgaagc
    12901 tgaggttcat tttattctgg agttcactcg gcgcatttaa tccaaatagt cttcttcctt
    12961 ctgatgctgc ataataagct gtatcaattc cttcctgaac gtcaccttta gtttcagcaa
    13021 ataccttacc catttctctt gtcatttcaa atgcgatctc atcttttctt tcagacattt
    13081 tatctcctac tacttttagg atgtcacctc ttttaggagc aggtactaga cgccatttat
    13141 caaaagctgc tttagctgct gatacagctt cgtttacgtc atctttattt gaagaaggga
    13201 aaataccgat caggtcttca ttccatttcg ccggatttct gttttcaaat gtttcactgc
    13261 cggaagaatt tttccattcg ccgttaataa gatttttaaa tatttctgcc attttattaa
    13321 attatctcct tttgttttat aatcagtcaa tttaactaat aagatgtttt atagaaattg
    13381 atttttaaat actttcataa aaatctattt attataatct gaagatcttt taaaaatact
    13441 tacaaaatac aatttgaatt acgatcataa ttcaatattt taatttttca aaaaataaat
    13501 ttaaaatatg aaacgatctg aattagcaag tttgcctgtt tattttggag attatataaa
    13561 ggaagtcgaa gatatagatc tgatatctgc tttggtgaaa tatggtccgg agatgtttgt
    13621 taaagatctg ccgactttag aaaagatcgg cggaaatacc tatgctgaga ataaatggac
    13681 gatcaaacag atcttacagc atttggctga caccgagaga gtatttcttt acagggcttt
    13741 gagttttgca cgaaaagatg aaactcctct accatctttt gatgaaaatt tatatgctga
    13801 gatttctaat tcaaacagaa gaagtttgaa tgagatactt actgaatttg agagtataag
    13861 aaattctgct ctaacaatgt ttcagggatt tacggaggaa atgcttttga gtacgggaaa
    13921 ttgttcggga aaggaagcat cggtagcagc aataggattt acaatcgccg gacatacatt
    13981 tcatcattat aaggtgataa gggaaaggta ttttttgttg gcggattgaa gttagttgat
    14041 ttgtggattg ggatcaacca accgttttaa cggtttatga tgagtaggta tagaattata
    14101 gatgaggttg tggtgttagt ggtattggat caaccgtttt aacggtttgt ggtgagtcgg
    14161 tatagaagca tagttgagta tctggtttta tattaatgcg gaaatttgtt aaagcaagag
    14221 aatcgtgaat actaaagagt ctaatattga cataaaccgt ttaaacggtt tatgtccgac
    14281 cagttcagta tctaaattca tcttactgct aattttaatt ccggagtttt ataaaaaaaa
    14341 tatcaaactt catctacaaa tttcagtgtt aactaaaata aaccgttaaa acggttatcc
    14401 tcatttcaat aacaccaact tctttgtcat caccacttct ccaccattca ctgtcaacct
    14461 gtaataatat actccaccag ctaaaccgct accatcaaac ttaacactat actcacccga
    14521 tccctgcttt tcattgacca aaactccttt aatcttcccc tggatatcat atattttaat
    14581 ctctgtataa ccagactcat ttaactcata tctaattacc gtctcaggat taaacggatt
    14641 tggaaaattc tgatgtagtt taattcttac aggaatttca ttctcgacgt taaaaatttg
    14701 cactgctcct gtatcatttc taattaacaa tctatccgtc tctgtagtct gctgtgtcgt
    14761 ccgtctgttt tgcaaataca aatccattag tccgtcatta ttaagatcag ttccaagcac
    14821 accaattcct tctgcaactg tattttgagg gagcactata tctgtaactt cagtaaaaat
    14881 tccgcttccg ttattttcaa atattttcat cggcctgtta aaaaaaatat ttgaggttat
    14941 cagatccgga tcattatcat aatcaatatc tataaatatt ccttctgttg tctgttcatt
    15001 atcaaccggt aaattggttg atgttatatc tgtaaaaaaa ccgcttccgt tattcaataa
    15061 aagcctgtcc tgtcttgtcc tgccggatat gaatgcaaca tttgcaaaaa aaatgtcaag
    15121 atcctgatct ccgtctacat ctcccaaagt gactttcctc gtctcttcag tcaatgatgg
    15181 cggaagtctc tgctctgtat catctgtaaa tactccgctg ccgttattta tatatatttt
    15241 gtttccgtct tcatttcctg ctatcagatc tttatctccg tcaccgtcta tgtctgcaag
    15301 tttcagatcc tgatttacat tggattcagc aggaagtctc agcgaagttt catttgtaaa
    15361 aatagaatca ccgttattta tcaatattaa atcctgacct gcatttccta atattatatc
    15421 tggaagtgaa tcattgttaa tatcctctgc cagtacagca tttgcagtgc taccaggaag
    15481 tctgttattt aaattactaa aacttccatt accgttattg aaatataatt catgtattgc
    15541 attatcctca cttgcaaata caatatcatt aaatccgtct ctgttaaaat ctgcaattcc
    15601 aatatcttca ctgtcgtaat tgaactgtgg taggtttcct aatgtaccat ctgtaaactg
    15661 tccgttgccg ttattgataa gtatcttatt cggggcaaat tctcttgcaa gcactatgtc
    15721 cgggtcaccg tcattatcaa gatctcctgc tgctgcatcc attgtcggac ctgttacagc
    15781 ttgtattgga aggtttgttg ttgttgcatc agtatataaa ataattcctc gttgatttgt
    15841 aaaagacatt gtagaaatta atatgaaggt aaaatataaa atagttttca taatatttta
    15901 aattttttat ttttaagtaa gtttttttaa aagatattca aattatgctg aaacaaaaac
    15961 atgtttataa ggattttaag ttataaattg cattaagttt ccattttatt taatttgttt
    16021 taaaattctt ttgatgcaca gaaagacagc ccttattttt atattattct cactggttta
    16081 ttttgctccc ggtaataaag ccgtttctca ggataaatgg tggaaagaaa aaaaatttaa
    16141 gaatgaacat cttagggcaa aatatgacct ttgtaaaaga acatttaaag atattggact
    16201 gggtttttct atcagtaatg taaatcatat taatcagtat tttgatgaac aggtttatct
    16261 gaacataatt agcacagata aagggtatta cagtcccaat caggcagaaa ttattatctc
    16321 ggaatttatg gattatttta atgttgatga attcaaattt atgacttcaa aaagatataa
    16381 cacatatgct tttgtcaatg gtaaatactc ttaccgattg ggtaatggaa agagaactct
    16441 tgatgtaact atttcattga aatatctaaa tgataagtgg tatgtagacc agattaatat
    16501 taattgatta aacaattcga attttaaaaa tctaatttta tttaaatcat tttagatttg
    16561 gagcagataa atagaaaatc tatattgaat ctttttgaca ggcggttaaa aattttgatt
    16621 ttaatcgctt ttttgtttat tgcatggctt gctgttgcca atcttttttc agattatatt
    16681 aaatctgaaa ctgaaaaaaa ctgggatgaa atttctaagg aaaaaaatga acaaagagtt
    16741 cagatcgtaa atagactgtt tctctccgac ctggataatc tgaatgaact ttcaaataac
    16801 atatcaaaaa attccgaaat tattgacctt atcagaaaaa gtgaatcaaa aaaattattt
    16861 gaaacactta taaatctgaa actgagtaat aatacgcaat atgaatttta taattcgcgt
    16921 ctcgaactcc tggcattctc cggaagaaaa ctcggttccg atttttattc tcttaataaa
    16981 tctataaacg gaaaaagatt ttctgtccta aaagaaattg ggttctatac atatctcatt
    17041 atatactctc cggtctatga cccggaaagt cagtctgtta ttggagtatc tcttaccgcc
    17101 gaactcatag atattaaata tcaaattgac aataaatttt ttaaaaattc cggtctgtta
    17161 tatagtatca gcaaagaaac ttctgtaagc cctgaactct ttccctctaa cgtaatttcc
    17221 ggtaaaatag aagtgaattc tcctaatccc gaagatttta ttcagataga tcttaaagga
    17281 cttgagaatg agattattgg tgttattctt attccaaaat ataacgaact gaatcatgtt
    17341 tcagagttac atgattttac tttaaggctg aactcaattc tcatttttgg tttatctgta
    17401 ataatttttt tactgaccta taaatttatt ttcaaaatta attcttccct gttaaagttt
    17461 attctattct ctgcagtgtt gatctttttt agatacttat ggctccagtt tgattttcct
    17521 tcaaaaactt tcaattcaga tcttttctct ccgggttatt atgcttctgt cttcggtaat
    17581 ggtatagcca aatctattgg agaattattt gttacttctg ttgtcgttct gattattgct
    17641 ttatttggat ttaatactgt tttgagaaat aaatttgttt ctaaaaataa ttttaatttt
    17701 ctaaaagcat taaaaattat tttttcagta atactttttt ttgctctcat ttatggattt
    17761 ggtgccgtga ttcagagcgt tgtatttgat tctaatctga agttttttga taaatcaaat
    17821 atcattcccg ggcctgaatt gttttttatt cagcttgtca ttcttatcct tactgtttct
    17881 ttttttattt ttctattatc tctgattatt catatctata aatattcact gtttcctgtt
    17941 ttcagtaaaa aaacaattag aaattttgct cccctgttta ttctgcttat tttacttctg
    18001 attaatcaga tctttactgt tcttgatatg aatttttata taaatacctt attcagaatt
    18061 ataattatag tttcagtttt cctctttggg tcatacttag gatggaaact ttataaattt
    18121 aaaatttaca atgtctattc actgaaaaat ttttctgtcc tgattctgat ctgtattatt
    18181 actattccgg gtattctgct tgaaaatatt acttcacagg aaacaaggta tgtcgaactg
    18241 atcggaaata agattgccga taaagatgat gataaaataa aatttctact gcttacagaa
    18301 ttatcagata tttctaacga taaaaagatc gagaataata ttcgaaataa aaataaactc
    18361 aaggaacttg gattttctat ctggaatgaa agtaaattca gtgaagaaaa tttgaatact
    18421 tccgttataa ttttagatac aaacagaaaa gttgtaagtg actttatgtt taattctaat
    18481 gcgatgatac ttgattcaat tattgctttt gcaaataaag aatattttat aaagaaagaa
    18541 tctcttgaaa atattcccga ctcctctgat tttacggact ccctatcctt tgctgatgat
    18601 cctgaaaatg aagaagaaat ggatttttct gaagaaagtt ttaaagaaga tcttaataat
    18661 ctttttatta cagataaaat tcttatcctt aataatgaag aagaaaatta ttatctcgga
    18721 attgtacctg ttgaaagagt agatctaaaa aatactatat acgaaaccaa tctgggttat
    18781 ttacttgtcg cagttcagta tgaatctaaa aatcttctgc agcaatcttc agtacagata
    18841 ttcaaaactt attcgcggga taatctgttt gacaaactta tttcagaacc tgtaattacc
    18901 gaatatgtaa acggtgagat tgtaagttct acaagccgtg atcagagtaa atcaaacaca
    18961 ctctctctta atgtattccg tgatgctgtt aaattcaaag atgaaaaatc tgactggaga
    19021 tatgaagtaa ttaacaatga aaaattcagg tcattctata tactagatcg aaacatagat
    19081 aaatctatga atgaaaggat ttattccgtc agtctgaaaa gaaatgatgt caagctgact
    19141 tttttctttt atttaaaatt tactttgttt gcatttttaa tatatatggt tgtgtttgtt
    19201 ataaaactac tccagatctt ttttagcctt aaagaactaa agccaaactt ccgtgaaaaa
    19261 ctttttgcat cattcttttt tgtatccgta attcctattg tcctgcttgc tctatataca
    19321 agaagtttta taaaagaaaa atatgatatc aattttcaaa atcagatcat ctcagatctg
    19381 aatcttgttt cacaaagtct caagaatctg aatctaaacc aactgagttc tgactctcta
    19441 agtgtccgcg gtaaaaaaat atttaccgct aatttatttc cgtctgataa aaattttaat
    19501 ctatacatta atacagatct tgtctctact accaatgatg aactttataa atctgatctc
    19561 ctcgattcaa gagttgatgc cgaagcttac tataatatta attatatgcg gaaagatttc
    19621 tttataaaaa atgaagagat aggtgcatat tctttcattg taggttataa accttacttt
    19681 gatcaaaaaa ataatgttgt tgggatcatg tcgtctcaaa cactctataa acaaaatgaa
    19741 ctcagcgaag agttaacaga aattcttact tttatttttg gaatttattt aattgttgtg
    19801 atcatacttc ttgtttttgt ttctattatg actgatagat tttcgcagcc aataatgaaa
    19861 ctaaaatatg ccaccgaaag aatttcaaaa ggcgacagca atgtagcaat agatatcaaa
    19921 cgaaaagatg agatcggaaa tcttgttgat tcttttaata ttatgtcacg ggaacttgaa
    19981 tcttcaaagg taaaattaaa aagagccgag agagaagcag catggagaga tattgccaga
    20041 cgtgtagcac atgagattaa aaatcctctg acaccaatga aactttccat tcagcattta
    20101 tttgatgttt atgaaaataa aactcctgaa gaattttccg aagttctgaa aaagacaaga
    20161 aacattatca taaatgaaat tgataaactc aataaaatcg caactgaatt ttctgatttt
    20221 gcaaagcttc ccggaaaaaa ttatactgaa acagatctga atgagatcat aggagaagtt
    20281 atttcattat ataaactttc ccccaatatt gaattcgttc aggatctgaa tagtaatatc
    20341 ggatttatct ctgctgataa acaggagctt aacagggttt ttcagaatct tgtcaagaat
    20401 tctgttcagt ctattggtgt aaatggaaaa attgaaatat caacaagaga aaacggtgaa
    20461 tacataattg ctgaagtaaa agataatgga tgcggtattg atccatctgt tatgaaaaat
    20521 ttatttaacc caaacttctc aacaaaaacc acaggtatgg gactaggatt atcaattaca
    20581 aaaaaatctc tcgatgatat gaaagctgag ataatgtttg aaagcattgt aaacaaaggt
    20641 actactgtga tcataaaatt taaaaaaata aattctaaga caaacaattc ttaatttgaa
    20701 taaatacctt tccagattaa tattttttat tctcttgaat tttctgttca gttgcgcaaa
    20761 tcaactgcct ccgtccggtg gtgaagatga caccaatcct ccaaagctta tatatatttt
    20821 tcctgaacca aactcaactt tagtaaaagg aaataaaata atttttaaat ttaatgaata
    20881 tgtggacaga agaagttttg aagaatcctt ttttatttct ccccggtctg caggtgatta
    20941 cagctttgac tggagcggta aagaagtcga aattaatttt gaaaagaaat ttgagcctga
    21001 cagaacttat gtaatgatca ttggcaaaga ttttaaagat cagcgggggg gtaatactct
    21061 tagttcacct gtcactattg cattctctac cggaagcagg cttgacaaag gcactttttc
    21121 agggaaagta tttgccggta attatgaccg cgtaaaagta atgatctatt tcagcaaaga
    21181 cactacatca gtattttcag atccggcaaa ttctcaaccc gattatatta ctcaggtaaa
    21241 tcttgatgga acttacgaat ttactaatct gcctaatgga aaattccgtc tgtatgccct
    21301 tactgatgaa gacagaaaca atgtctatga ttctgatctt gataaaattg ccatgctgcc
    21361 cgatgatata aaactttttg gagacagtac ctctgttagt gatctgaatt ttataatgga
    21421 tttaaaagat aaaaataatt acagcagctt atttttaaat actctttctt cagattcatc
    21481 tggcttaatt tattctaatt acaattattc aggatcaaac tttatcacac ctgagcatag
    21541 gttttatttt tattttaaaa atattactcc tgataaaaaa gaagttgttg aaaattttat
    21601 actctcagat acatctgaca ggaaagttta taaacccatc tttaactgga taaatgattc
    21661 tttgctggaa gtatttactc aggaaaaact taaatttaat tctgtgatat ccctcgctat
    21721 tgatattaag ggaacaggga acagcagatt taccggatca tacaatgtat atgaaaagga
    21781 taaattcgga aaaatttcag gtgatgtaaa aagaactgat ccagatattc ccgcaatact
    21841 aacactttac agtttagcta aggatggtcc catatataca aaagttatat ctgatactac
    21901 tgcatttgaa attgatgata ttcttgaagg gaaatatata ttgtttgtat ttatagataa
    21961 taatttgaac gggacttttg acaaaggtaa tgtaaaccct tttaaagctc ctgaaaattt
    22021 tattatttat gataaggatt ttgaaattaa ggggaacagc agtattgaaa aaattttact
    22081 ggaattttaa aacagggtaa tttctgaaaa aattaattat tttttttgtt gataaatgat
    22141 cttatcgtga aaaaaagatt ataaagtaaa agctccgggg aaatgttatt atcgagactt
    22201 ttaactgcat cttcaattga acaaattatc agataattat ccgattcata attatttgta
    22261 aatctttcta gtctttcctt tttatctctg tttattatta actcttcatt tccgttatca
    22321 aaatttataa tatccctgaa ccaaattatc agaaaaatga ggaagttttt aattctgacc
    22381 ttatctcttt ttccaataat aaaatcaata ctactgctca gttttagatt attaactgta
    22441 agtaaagcaa ccaatgtatc cataactttt tccctgaggt caagataatt attctccaat
    22501 atttgattgc atcttgaaat actaccatca gatatgtctg caaagaaagc tgcattttca
    22561 tcagaaatac tgtgcttctt ttttttaaga taatctataa tgtatttttt ctgaatgctt
    22621 tcaaatccaa tcttctggca tcttccgata atggtcggca gcaaagaatt taatctggaa
    22681 gtggttagta taataacgga atcttttggc ggctcttcta aaattttaag cagagagttt
    22741 gcactttgag gattcatcat atcacatctt gaaataagaa acactttttt cttgcctgac
    22801 cttcctgtca tatatatttc tcttttcatt tgtctgatac tggaaatacg aatatcgttt
    22861 gcacgtggaa ttgagatggt gtgatattta tcttcagctt ttaattccat ttgagataaa
    22921 taaatttgaa aatcctcctt atctaatttt tcaagcggat ttgaatcttc atcagaatca
    22981 ctttttccag ttggaagtgc agtaatgaat ctaagcatcg gtgatttcag tgaatcaatt
    23041 tcaatacaac ttttgcatat atcacaggct tctgtgtcgt ttaccttgtt atcacagttg
    23101 agcagctttg caaattcaac agcagctgca tccttacccg taccttcatt tccataaaaa
    23161 atataagcat gagagatctt accgccttta taaaaattgg taaaaatttc ttttactctt
    23221 tcctgtccaa ttatattatt ccacatatta aaatgtattt cctattccaa attgaaaatt
    23281 atatttatca ttgaaattag atccgggtgc aaagatccag ttagatcctc caataggtcc
    23341 cggctgcggg tcataaattt tgaatccaac atcaaatctg atcgctccaa ttattgtgta
    23401 atatctgatc cctgtacctg ctgctattgc gatttcattt agtttgaatt ttcctatatc
    23461 cgaccataca tttccataat ctacaaatcc aacaaatccc agatcccgta aatattcgtt
    23521 gctcattgat agaaatggct taactctgta ctctactgta ttttcatatg tgaaatttcc
    23581 accgagatct ttatcaggta ctattcccaa ctgcctggct gcccagcctc tgacactggt
    23641 actcccgcct ccaaaatatc tggtgtcacg aggtactctg tctgaagaaa attcctgtcc
    23701 taaaaatgta aatgagtttt gaccatattc agcgattatt cctgccagaa gtttaaatgc
    23761 aagtactgca gtaacgttta cttcacggtt gcttagattt atatatgctg aacttaaatt
    23821 tgtaaatttt gaataactgt aagacgctgt attaaaaatt tttcttatga ctccggataa
    23881 taatccgctt tcttcgatct cataagattg ataatttcct gctgaaggaa aaacaatgtt
    23941 attaacagaa ctgtgaataa tggtaaaacc aaattttgat ataaactcat tgagatcgaa
    24001 attaggtaag agaatttcct cttcctctga tactatatca ttgttcagta caaatctcga
    24061 gttttcaagc tcccatctga attgcaggtt atttacataa gtataatcgg ggagttcgta
    24121 aactacgtta aatgaatttt taaatataga ctggattata tcgtcttgta atctgtattc
    24181 ataaccaata ttccagttac ctgttataaa tctgttaccg aataaaaatg gctgactaat
    24241 tgtgtttaca aattccagtc tgttattttt aggtgagttg taataaaatc ttgcacgcga
    24301 tgtaagtact ctgccgtcac caaaaaaatt tagatcgtta aaagatacac ctgtgcccat
    24361 ataaaaagaa ttctgaaagt aatatccgaa tacttcagga gttacatcat atttgttacc
    24421 tactacagca ttaacgataa aatttatttt atttgaattt tcatttatag tatcgattac
    24481 aattcgggga ttttcaagaa tagaaaaacg tgtcaggtta atttcacttt tcactatttt
    24541 acttttatca tacatctcgt tttctttgta ttccaattct ctgcgaatgt catcttcagt
    24601 aatattgtat ttatcatttg taaatttaac tactgttttt ccaaattcat atttcttccc
    24661 ggggtctaaa acaaatttta tattaacttt ataaaacagc gaatctatat tagttacata
    24721 tttaaaaatt tcaggattca gagctgaagc tgttgcatat ccattatcct gaagcagatc
    24781 catgatcctt cccctttcaa gattcacact gtccttggag taatacctgc cttttttggt
    24841 aaatgaattt ttttgatttt gtatcttacc tgtcagatct gcggaaatgc tgtctattcc
    24901 tgtatagtta atatttaaat attcatatct tgcattttct tttacagtaa aagtttcatt
    24961 aatttcctta tcttctatgt ttgtaaaaag tcccgtatcg acttcagcat caaaaaatcc
    25021 gttatcaaaa taatattttt taattctctc aacatctaat aaaaaagttg tcatatcaaa
    25081 ataatcaccg gattttgaag caagcagacc tttaattgta ctctcgtcaa aagtactgct
    25141 ttcaatgaac ttaatattga tgtcatctat ctcataattt ccttcaatag atgtatcttc
    25201 ctgacattca gacataccgg tataaagaaa caatattccg gtaatcagaa acagccgcag
    25261 aaaattattc atcatttaag tttaataaaa aagacccaat aatttacatc attgggtctt
    25321 aaataaaatt taaacacctc aaaaatcttt actttcttca tcataaaaaa agtcttcatc
    25381 tgttggataa tcaagccata tctcttctat actttcaaaa ggttcttcag tatcctcaag
    25441 atcattgaga ttgtttatta cttcatttgg tgctccggat ctgatagaat aatctatcag
    25501 ttcaaattta tttgccggcc acggagcatc atcaagataa gacgctaatt ctaatgtcca
    25561 taccatatta tttatttacc tcctgcattt gcttttgtat tttttattta agttaattaa
    25621 aattgtgtta aatattattt taaaacagat cagtctctta atctcggatc taatgcatca
    25681 cgtaatcctt caccaataag attatatgta actacagcaa taaagatcat catacccgga
    25741 aaaattgcca gccaccaagc gtttgatgca cctcttgctt cattaagtaa agaaccccag
    25801 gtaacagttg tagcacttac tccgaatcca agaaagctca atgctgcctc agttaaaatt
    25861 gcacctgcaa ttgcaaacgc tcccgaaacc agtaccggtg caattgcatt cggaattaca
    25921 tgtctgaaaa ttatctgttt attcggtaaa ccaatagagc tggcagcggt aatatattcc
    25981 atattcctta ctttcagtac ttcacctctg atcagctttg catcacctgt ccaggatgta
    26041 ataccaattg ccgccattat ataccagata ctcgatccgt aaattgcaac tattgttata
    26101 ataagaaaga agaccggcag tgttaccatt atttctacaa atctcattat gatcagatca
    26161 actttgcctc cgtaatatcc ggcaagcgat cccaatacaa ttccaatgac cagagcaata
    26221 cctgccgcga caaatccaac tgaaagtgat actctcgatc cgtgaatcag acctgcaagc
    26281 agatctcttc ctattgcatc ggtgccgaga taatgatctc ctcccggaga cctgagatta
    26341 tttgcaagat caatttcgct cggaccataa ggtaccggtg tccacacaac actttccagc
    26401 tttccttcat catcaagttt tttccagtta gcatttatca tatcaggctc ccatttgcta
    26461 atgcctatag catttaaata atctttgaca actggaaaat atgtttcacc tttgtattca
    26521 gcataaagtg gtttactgtc tgcaagaaaa tctgcagtaa ctgcaattac tatcaaaaac
    26581 agtactatat acatcgatat catcgccagc ttgtttttct taaactgatg cttaaccaat
    26641 gaccagtaag aaacaccaac ttccttctga gattcaagct gagatttact cattccgttt
    26701 ggatcaggct tatcatcaat gtttttttct ataaactcat ttatctcgtt tggttgcttc
    26761 atatattagt tatgctgttt ttttagaaaa tgcgattctc ggatctacga atgaatataa
    26821 aatatctaca gctaaaatcc ccactaccgt taatacagaa gtcagtacaa gctcggccat
    26881 tatcagcgga taatctctgt caagaattgc ctgaaatgca agctgaccaa taccgggtat
    26941 tgaaaaaatt gtttcaataa ttacacttcc gccgatcatt gcaggaagta atccgcctaa
    27001 aagagtaatg atgggaatca gtgaatttct taaagcatgt ttcattacaa ctttcttttc
    27061 cgataaacct tttgcttttg ccgttcttat ataatcctgt ctgatgactt caagcattga
    27121 acttctcatc tgtcttgaaa taaaagcaaa gcttgttaaa ctcgaaatag taacaggaag
    27181 gaataaatgc atgaacctgt cttttacctg tcccataaat ccaagcgaat cgaagtcttc
    27241 agatcttatt cccgcagttg gaaatatttt caaatattcc acattacata aaaatactat
    27301 tgcaagtgta gctacccaaa aatttggcag tgaatataat acaaaaagaa acactgtagt
    27361 tattctgtca gtaaatgagt attgccggac agcactgtat attccgatgg gaattgcaat
    27421 caaatatatc agtaaaaatg acaaaagact gatcgtaact gtcacaggta acctttccag
    27481 tattttatct attacaggtc tgtcgtcttt aaaagaattt ccaaaatccc cttgcagcat
    27541 atttccaatc cagatgaaat actgcatgta caatggtttg tcaagtccga atttcttttt
    27601 gtaaaattct tttgcctgct cattcattag atttttggaa tctgtcttct gtgcttcctg
    27661 agtctgtccg atcttcattt cagtcggatc acccggcgcg agcctgcaga caagaaatgt
    27721 tatcattgtg ataatgatga gagtcggtat aaaaagtaat atccttctta ttacataatc
    27781 tgtcattttt actaattagt gttattgatt aatttttgct gtctgcaata taaatcacag
    27841 acagcaataa tattatatat taatttttat atctttgtgc attagtcgga gtccaccatt
    27901 cattaagtaa tggtgaatcc ggatatgcat accatcttgt attattaaat ctatcactgt
    27961 aaacatatct tgatggctct gcccagagga atgtcgtcgg ctgatcttca taaatgatct
    28021 cctgccattt ttttaggagc tccattcttt ttgcatcatc aaactcgact ctgttttgct
    28081 cgataagttt atcactttcc ggatttatat aactgataaa attacttcct tcacctattg
    28141 cctgtgagga atgccatatc tgatacggat cttcaggtgt gacagaaagc tgccatgcag
    28201 aataacatgc atcgaattca tgcttcttcg ttttatccaa aaatactgac cattcatatt
    28261 cctgtagatc tgcctgaatg ccgaggtttt ttagtgactc aattactaca agcatgacct
    28321 tttttctttt cggattatta ttgtttatga atgtaaattt aaaatctgtt tttttaccgt
    28381 ctattacttt atcaagaatt ccgtctccgt cagtatcttt ccatcccgct tcagcaagca
    28441 gctgttttgc tttctcagga ttgtaatcaa tttcgggaag atcattattg taatatttac
    28501 tttctttaaa tacaggagtc tgaactttgg tacccattcc gtaaacaatc ttgtctatca
    28561 ttgtctgtct gtcaattgca tagctcagtg cccatcttac ttttttatcg gcaaacaatg
    28621 gattcttatt attccatgca ataaatatat aaacaggttc ggatacaagt gctttcttta
    28681 gattgaattt ttcaggatcc tttatatttt ctacaaagtc gatcggttgt atgacataca
    28741 tataatcaac ctctttgttc tttgcagcta ctacagcagc attctgatcc tgaataattt
    28801 tgaaaacgag tttagaaggg aaattcggaa tatctttttt attccagtaa tcttcatttc
    28861 tctgtaaagt gatcgactga cctgtgagcc acttatctaa tttataaggt ccggagccta
    28921 ctacatatct cggctctctt gaaacttcct gcgaattcag aaaatctgca tacttctgca
    28981 tatcgggata ttttaaagga tcaagacttt tctgtgcttc atcaagctgt tcccatgtaa
    29041 acttttcatt catattatct ttgtcgagaa tatgctttgg agtaattgcc atacttccaa
    29101 gtgcatagac agctctgaaa tatggtttcg acatattgaa tctgacctta tatttatttc
    29161 cgtcaacaag ctcaacgctt ttcagatcaa taaaataatt tctaagtgcc tgagcatctg
    29221 tgaatggatt cttgatagtt ttaactgtaa aaattacatc ctcaccagta aggggagtac
    29281 cgtctgaaaa tttcacatcc tctttaagat caaatgtata tgacatttta tcttcactta
    29341 tttcgggaag tgattttgca ataagaggta caagatcata agtttctctg ttcacatcaa
    29401 gtaatgtttc aaatatgtaa atataaatac ccgaagctga agcatcattt gtaactgttg
    29461 ggttcagttt ttcagcatct gacatttcct gctttatgac ccagtcaccg tcatccgcat
    29521 tttcaatatt tttcggagcc agttttgaaa gatctacagt agcattctct ttactttctg
    29581 ttgtttcctt atttcttccg catgatgaaa atatcaaagt gaaaattatc atcatcggta
    29641 atattacttt tattggatta tacattatta ttattgttta gatatttttt tattaaaaaa
    29701 attaattatt cgaaatcatg ttcaaaatat tctgtagctt tttccaagcg gtcatctctg
    29761 ctgtataaac tatctttatc agatttcctt gagatctttt cctgaagttt tttacggaat
    29821 acttcggctg cattataacc ataatgttta cacaaataat tcaacgcaat tacttctgct
    29881 attacagtta tattctttcc gggaaagatc ggcaatttaa taagctcaat atcaacactc
    29941 agaagatcta cagttgtatt atcaagacct gtcctgtcat aagctttgtc tgtatcccag
    30001 tcttccagct ctactattac ttcaactctt ttctgatacc ggatggatct tattccaaaa
    30061 attgtactta catctataat tccaagtccc ctgatctcca taatatgttt tgacattgcc
    30121 atccccgaac ccatcagtac accttcacct tttttggaaa ctacaactac atcatctgcg
    30181 acaagtctgt gtcctctttc taccagatca agtgctatct cacttttacc aattccggac
    30241 tttccagaaa ataataaacc tactccatat acatccacaa aagatccgtg cattatgatc
    30301 tgagtagaaa actggtcgtc aaaaaaatct gatataaaat aagaaaattt tgtggtagga
    30361 taaaccgttc tgaaaattgg tatatgtttt tcgtcagcaa gttttataag ttcgtcagga
    30421 actttgttat tgtttgtaat tattacacac ggcacatcaa atgaaaaaaa tacctgaagt
    30481 gattttagtc tgtcctgagg tgaaagtttt tcaagataat gtatttctgt attgccgaag
    30541 atctgcactc tgttaaaagt aaacagctca acaaaccctg caagagcaag ccccggtctg
    30601 tgaatatctt tttccgtgat ctgtaacctg gtatcaattt cttcatttga gatcttttca
    30661 agtttaaatc ttcccttgaa ctcattataa aggaattcca ctgttatgaa gtttttcttg
    30721 atctctttta tattttcaaa cctaccgggc aattttatat agtctttgtt atttcttttt
    30781 tagtgttgtg cttatctgtt ttgttcttgt ccttaatttt taaaatttgt gtctcgatct
    30841 tatcaattgc tttatcaaca gattttacaa aatcatcgct gctctctttt gccatcagat
    30901 ctttacttcc aattttaact aaaatttcgc agtattttat actgtttatt gctttctcaa
    30961 aaaaaagtat tacatctgca tatactatat cttcattata tttgctcagg ttatcaattt
    31021 cacttctaat ataatcctgt aatgtatcat gagccttaaa atggcgggaa gtaatattta
    31081 cttttatcat tttttcaatt ttttaagggt taataataat tggtttcact gtttttgcga
    31141 atatgctaaa ctaaatattt aatttcaact atatatttat gattatttaa gaaacctttt
    31201 tgtaaaatat aaatcctcaa caatttaaat ttgaaattaa tcaactcggt ttatttcaaa
    31261 atttaatttt tatacaattg tctgtttgca ggtacccacc attcatttaa tagaggtgag
    31321 tccggatatg cataccatct cgcattttta tatctgtcac tgtagacata ccgtacaggt
    31381 tcactccata aaaatgtgac cggctgatct tcataaacca aaagctgcca tttatcgaga
    31441 atctcttttc ttttagcatc atcaaaagta agtctgttct cttcaagtaa tttatcagct
    31501 tcggggtttt tgtaactgaa ataattactg ccctcaccaa tagactgaga tgaatgccat
    31561 atctgatagg gatcatctgc tgtcacagaa agctgccaac ccgcgaaaca tgcatcataa
    31621 ttatgttttt tgatcttatc taaaaataca gaccattcat attcctgaat atctgcatta
    31681 atgccaagct gtttcaattg ttctataagt attaaaatga ctttctttct tttgggattg
    31741 gtattgtttg taaatgtgaa tttgaaatca accttattac cgtctatcat tttatcaagt
    31801 gtaccatctc cgtcagtatc tttccacccg gcttcagcaa gcagatcttt tgctttgcca
    31861 atatcataag gaattggcgg aagatcagta ttcatgtatt tgcttttaaa aaaaatatga
    31921 gattctatat ttgttcccat tccatataca attttatcaa caatggattt tctgtcaata
    31981 gcataagcca ttgcccatct gactttcctg tcagaaaaaa ttggactttc attattccat
    32041 gcaatgtaat tatacgatgg ctcgatgacc agtgcttttt taagctgaaa cttctccgga
    32101 tcttttatat tctctacaaa atcaatcggc tgaattacct gcagctgatc gacttcttta
    32161 ttttttgctg ccactactgc tgcattctga tctttaattg ttttgaatat aagtctttga
    32221 gggtaattag gaagctcgga tttattccag taattttcat ttcttacaag tgtgatagac
    32281 tgacctgtaa cccatttttc aagtttgtat ggtccggatc cgattacata ttcaggggaa
    32341 cgtgaaacct cttccttgtt aataaagtcc gcatactttt gcaatcccgg atactttaca
    32401 gggtcaaagg ttttaccggc ttcttcaagc atttcccatg tgaatttatc ggtctcatta
    32461 tttttatcaa gtatatgctt tggaatgatc tccatatcac ttatagaata aattgctctg
    32521 aaatatggct tggacatatt gaatcttact ttaaatttat tgccatctac taattccaca
    32581 ctctttacat ccataaaata atttctggat gcctgtgcat ctgtaaaagg atttttaatt
    32641 acttttactg taaaaataat atcttcagct gtgagaggtt taccatctga gaaagtgacg
    32701 ttttctttca gatcatatgt ataggacaga tgatcttcac tgatctcggg cattgacttg
    32761 gcaatgagag gttttaattc atatgtgact ctgtccagat caagaagatt ttcaaacata
    32821 tagataaaga tcccggttgc agtagcatca tttgttacga tcggattaag tttttctgca
    32881 tcggacattt catgtttgat tatccagtcg ccgtaatcag aatttgcagt atcaatgggt
    32941 tgtaattcag ttatagggaa agattctgta ttgctgtttt gtgaggtttc cttatttctt
    33001 ccgcaggaga taaccagaac tgaagaaaat ataaaaaata caacaagatt tatctttttc
    33061 ataataataa tagcattttg atttcataaa attttttttg atagtaaata ttctaattga
    33121 tttattcaag tgaatgtaat attttaaaaa atgaaaaata aatccggaga taatgaacta
    33181 aattctaatg acgatagtat ttcaaaaaaa gtttactata ttattttaat cttttcattt
    33241 ctttggcttt tacttatttt tctcgctcct gttctaaaag atattggtgg ggtttttgca
    33301 gatatatcat cttatttata tatgtttttt tcacctgtat gtcatcagga tgatgcaaga
    33361 tcttttcata ttttcagtga aaaagttgca gtttgttcca gatgtttatg gatatatagt
    33421 gggtttacag caggtacggt attatatccg ttaaaataca aactcacaaa tgaaaactct
    33481 ccgtcggtaa tatttattat cgctgcatct gctctgatcg cacttgatgt attgctggac
    33541 ggacaggaaa ttattaagaa ctcgtttata tcaagatcag taacaggttt cataattggt
    33601 ctgactctgc cattttattt aataccgggc agtgtaaggt ttttttctga agtaaatctt
    33661 tttttaaaaa ataaatctgc cggataggca taatgcagtc aaacaataaa ttaacaccgg
    33721 tcataattgg tacattagtt atgactttca tagctctctt ccccattgta aattttctca
    33781 acatcctctg ctgctcggga ataatgatag gtggatttgc aggcgttaca tcatataata
    33841 aacagcttac taattcagga cagactctga cagccaaaga cggcggtatg atcgggatac
    33901 tcagtggaat actttcagct gtttttgtat cgggttttgg tcttctcttc agtttgtttt
    33961 caaatcaaaa tccaatgaca gaaataatga atgcattttc tgaatttggt tttcagatac
    34021 cggattcagc agcttattat cttgataaat tttcaaatga attcaatgat catggttttt
    34081 ctcctactct cacaattttt tcttttataa ctaatcttat aatttatccg ctattcggca
    34141 ccataggagc tttgatcgct gtgcagattt ataaaaagaa aggtaacgtt tgatttggga
    34201 tttatttcaa tagtaaaaac tggtccgtca gaatcccgaa agatcagaat atatttaatt
    34261 ataattttat tttttgtttt cttaacttca tatttttcac cggcaaaact gatcagacaa
    34321 tttattcctg atctttttaa cagagaaagc tcatgtattc tgttaaatgt ttcaggtatc
    34381 ccttgtccac tatgcggttt caccagatct tttgaagaga tattaaaatt gaatattgcc
    34441 ggtagttttt attacaaccc ttcttcggta tttatcttta catttttagc gtgtctgact
    34501 ttatttattt ttgtaattag tttttttaat tataaaataa aaatagtttc tgctgataaa
    34561 acttttttag cattaactgt tgttctcgta acagtctgga tagtaaatat tttttttgga
    34621 catcactaaa ttcagattaa tattatttgc aaaaattttt atgaaaaatt taaacacatt
    34681 taattagtta acttatgttt gaatacacag gatcacttca tatgcattcg gtcttttctg
    34741 acggttcagg tacagtagaa gatatcacca aagctgctgt cagttctgat ctcgatttta
    34801 taattctcac cgatcacaat acattaaaag caaaagacaa aggttatgaa aagtggtata
    34861 aaaaaactct cctcatagtt ggttatgaag tcaatgacag aaaaaacaaa aatcattatg
    34921 ttgtactggg gacagataag ataacaggta cttacgaaaa acttcccgac ggagataccg
    34981 gatgcagaat gactgcctct gaatatgtaa aagctgttaa tgatgaaggt ggttttggat
    35041 tcattgcaca tcctcatgag aaaagacaaa atcttcccga gcatcctcct tatccctgga
    35101 cagactggga ttgtaaagat ttcaatggta ttgagatctg gaatcatatg agtgaatggg
    35161 cggaagatct gaatgaatca aataaattcc agaggtttct gcatcctctg aaatcaatca
    35221 ttgcaccaca gcaaaagaca ctaaaactct gggacgatct aaacaaaaaa aggaaagtcg
    35281 tagcaatggg aggaatcgat gcacatgcac ataaacacaa tgtcatggga atggaatttg
    35341 aaattttccc gtataaagtt ttatttaaat ccatccgcac acatgtactt ttaaatgaag
    35401 aacttaaaaa aggaaatcag accagttata ttgaaagccg tgataaaata ttagatgcac
    35461 tcgcttacgg aagatccttt gtagtaaata attattacgg cagcggaaag gggttcaggt
    35521 tttatgctga atatgacgga gctttttata ctatgggtga cgaaattaaa tttgacagaa
    35581 caaaaaataa gaaaattatt ttcagagctc tccttccttc aaaagcaaag atcaagttta
    35641 ttcaaaatgg taaatgtact gatgagcttg aaggtatgga atgtatatgg gatcgtgatg
    35701 aacccggtat attcagagta gaagcatgga tggaaaacag gggatggatc ttttcaaatc
    35761 atataagagc aactgtttaa tgttcaattt taaatgtgca atgtattctt ttcactcttc
    35821 attttttact ttttactatt taattcttaa ttcctaattc ttaattccta attatttctt
    35881 ttaaggttta cctttattct ttatcttcaa aaatttaatt ttacaatact cagccttgaa
    35941 gataaaaaaa ttcaaaaata cgggattcaa ttcaaccaaa tcaattcatt ccaattgcgg
    36001 agtgtttggt gtctataata atccggatgc atctgttcta acttattatg gcctgcatgc
    36061 tttacagcac agaggtcagg aagcagccgg gatagtatca tgtgagcaga atcaaaacgg
    36121 agacaaaagt aaattcaata ttcacaaaga tctcggactt gtgctaagtg ttttttctga
    36181 tgacaaaatt ctgacggatg atttaaaagg tacatctgct atcggacata caagatattc
    36241 cacaactggc tcaggtgaca acagaaataa cattcagcct ttcaaagtaa attataaaga
    36301 cggaaacctt gcgctctctc ataacggaaa tcttacaaat acaaaatttc taagaaataa
    36361 acttcagaat gaaggcacta tctttcagac gactactgac agcgaactta tacttcatct
    36421 cattgctaaa agcaaagagt ctgatgttgt aaataaatta cttgatgcat tttcacaggt
    36481 ggtaggagct tattcgatct gcattcttac tgatgataaa ctttttgcaa taagagatcc
    36541 tcacggagta agaccttttt gttttgggaa ggtcgatgac agttatgttt tttcttctga
    36601 aacatgtgca ctggatatta taaatgctga ctatataaga gaagtaaatc cgggtgaaat
    36661 aattatgatt gaccgcgaag tagttgaaac gggaattgaa aaatcatttc aggcttcgac
    36721 agttgataaa tacaaacact gtatattcga atacatttat ttctcaagac ctgatagtac
    36781 agtgtttgga gaaaaagtgg ataaagtaag aaggacgata ggtaagaatc ttgcaatcga
    36841 aagtccggct ccagtaagag agcctgagga cacaaaaaag atcggaatta taaatgttcc
    36901 tgattcatcc aatactgcca ctttaggata tttcaatgaa atggtaaagc ataataaaga
    36961 tgtcaaacat gaaattggtt tgataagaag tcattatgta ggcaggactt tcattcagcc
    37021 cggtaaagat aaaaggaaaa tgaaagtgaa gactaaattc aataccgtta aaggagttat
    37081 caaagacagg aaacttgtcg tagtagatga ttccattgtc agaggtacta cttccaaact
    37141 tcttgttgac ttaattaaaa aggccgatcc aaaagagata catctgaaga tcacttcgcc
    37201 gccaatcgtt tctccgtgtt attatggaat ggattttcca actgcggaag agcttattgc
    37261 aaatcagcat gacagcaatg tcgaaggtat tcgtgaggaa cttggtgtaa cttctctttc
    37321 atatttgagt acagaaaaac ttctggagtc tgttccttat tcaaatccta aaacagattt
    37381 ttgtacagca tgctttacaa aaaattatcc aatacaaata gaaggtcttg aagaaatgaa
    37441 aaaagagcaa tttgacgatt aatattaaat tttaaataaa aaattactta caataatatt
    37501 tagcataatt attatgaata aagatcaatt aaattttcac aaatccagaa ggatagagtt
    37561 ttcgaagctg ataggcaatg atgcaatagc gctgatcttt ggaaatacac acagaaataa
    37621 atcatacgac ggtgattata aatttaagca atataagaat ttttattatc tgacaggatt
    37681 tacagaagca aacaccgctc tcgttattgc gccgggagga ttacctattg aagctgataa
    37741 gaaaaaactg accgcgaaag aaatgctatt cgtacagcaa aaagatgaaa gattggaaac
    37801 ctggaacggt aaaaggcttg gatataagaa tgtaaaaaaa tctctcggca tcgaacattc
    37861 cgatataaat ataaatcttg gtaagattct taattcacga tatttaggta aatacagaaa
    37921 gttctatgtc aattttgctg agttgatgaa gcttaccggt gaaatgaaaa aaattgtttc
    37981 agattttatg gaaagtctga acatcatcgc tccgaatgtt gagatcatag atgtttcata
    38041 tattttggga aagatgagat caattaaatc tgaatttgaa attgaaagaa tcagagatgc
    38101 ttcacatgtt tcggtaggtt catataatga aatacttgaa atacttgaac ccgggatgaa
    38161 tgaatttgaa gtgcagtcaa ctcttgaata ttattacaga tatcatggcg gagatgatat
    38221 tgcatatcat ccgattgtag cagcgggaga aaatgcctgc atactgcatt atgaaaataa
    38281 tgatcagact ttgaaaaaag atcagttgct gctgatagat tctgcttcgg aatttaatta
    38341 ttactgttct gatataacgc gtacatttcc tgtcagtgga aaatttaccg atgaacaaaa
    38401 acagatctat gaaattgtat taaaagctaa taaagattgc ataaaaaata taaaacccgg
    38461 tgtgaaattt tccaagatcg ccgagatgag tgataagata cttgcagaag gacttcataa
    38521 agccggactt cttaaagaca aatctaaaat aaaaaaatat tcgctccatg gtatcggtca
    38581 tcacattgga cttgatacac acgatgccgt accatcgtct cttacaaaaa cttctgatac
    38641 agatacttta aaacccggca tggtgcttac tattgagccc ggaatttatt tcacaaatga
    38701 tatgaaagag attcctgcga agtaccgggg aatgggaatc cggatagaag atgtaatact
    38761 tgtaacagcc aaaggatgtg ataatcttac tgaagcaatg gtgaaagaag tgaaagatgt
    38821 agaagaaaga atgacttctt gaaacaatta aaaattaaga attaggaatt aggaataagg
    38881 aataacttcc tatatttgtg atctgaaaag taaaatttct cttttgtctg aaacatgatt
    38941 aattttaaac ctctgatcaa aaaagttttt acttaacaat tttgataaac taaaaaaatt
    39001 gagtaaagta aaatctctca tgaaataatt ttatttcaca tatttttact gaaataaaat
    39061 ataaactctt accgctgttt aattctttga ttcaaaggtc tgatttcgat ctcatttata
    39121 agtgctctgt caggtagatt cacagcaagc aggcatgttt cagctacatc atcgggggat
    39181 agcaaagtct ctctgttggc tgttaatccg gcgcttggat ggtcgaagaa atttgtatcc
    39241 actgatcccg gacagatcgt cactactcta atattatctt ttctgacctc tgccatcaat
    39301 gatctcgaca atcccatcac agcatgtttt gtagcgctgt aaatggaagc agtcgcaaca
    39361 ccgttcttac ctccgataga tgaaatattg attattgtac ctgatttttt cttgatcatt
    39421 tcttttacac cgaacctgtt gcacagatac agacctttta cattcactga aaacatctca
    39481 tcaaaatcct tagttgttgt atctgcaaga tttgcaaacc ttccgaatcc tgaattacag
    39541 atcagtacat ctatcttttt aaattttttg ataacggcct gaaataattt ttttatatca
    39601 gcttctttgg aaacatccgt tttaataaaa tgaaaatccg ggtcttttat agattttgca
    39661 tttgttctgg atgcgtttat gactgtataa ttatttttaa gaaagatctc tgcgcaggat
    39721 aatccgattc cttttgaagc acctgtgatt actactgttt ttttcatttt cataaattag
    39781 gtttttttga tttgataaat tattaaggac aatctaagat taggagatca gaaataaaat
    39841 gttgaaagtt ttattttgct taatataaaa attttcatca ggatttgatg actcagactt
    39901 tttataaatt taatattaga agttttgcat tttttagatt tgaaatttaa tgacttagta
    39961 agtttaattg aattcaattc tgccaaacaa tttacctcca taaattaatt tactctctta
    40021 ccaaattttc ttatcttaac ttcccaaata aaactaatgt atcttaatct taatatttta
    40081 aatcccgaac agcgtaaagc agtcgaagag atcgaaggtc cgagtatgat cattgcaggt
    40141 gcgggcagcg gtaaaacccg tgtgcttact ttcaagatcg catacctgct cgaatctggt
    40201 gtcagccctt ttgaaattct ttcacttact tttaccaata aagctgccgc tgaaatgaag
    40261 gacagaattt caaaactggt agccacttca tttgaaaaga tttggatggg aacgtttcat
    40321 tcaatcttcg ctagactgct aaggattgaa gctgactatc tcggatttaa ccggaatttt
    40381 tcgatatatg atactgatga ttcggcaagt cttataaaac agatcatgat caatttatct
    40441 cagccgactg ataatccgac accaaaagct gtgcataact ttataagcaa tctcaagaat
    40501 aaaatgatac tgccaaaaga attttccact atggcaaaat caacttttga aaaaaagatc
    40561 gaaatcatct atgaagaata tcagaaagct ttattcaaaa ataacgcaat ggattttgat
    40621 gatctgcttc taaagccaat cgaactcttc agaagatatc ccgaaattct tgaaaaatat
    40681 caggagagat ttaaatttat tctcgtcgat gaatatcagg atacgaacaa agcacagtat
    40741 actataataa aattattgtc agacaaatat aaaaaccttt ctatagtcgg cgatgatgca
    40801 cagtctattt acaaatggag aggcgcggag atacagaata tatttgactt tcaggatgac
    40861 tttaaggaat gcaaaatatt taagctcgaa cagaattacc gttcctctca gaatattctc
    40921 acacttgccg gaaatgtaat aaagaataat aaaaagcaga tggagaaaga actttggacg
    40981 gaaaatgatg aaggcgaaaa aattcatatt atagaatcaa tgaccgaccg tgatgaagct
    41041 gtccgtatct gtagaaatat ctatgatgag atacagagaa aaaaaattaa cttcaaagat
    41101 tttgttatac tctacaggac aaatgtacag tcacgtacta tcgaagatgc cctcagaaat
    41161 aataacatcc cttatttgat catcggcggt gtgagatttt atcagagaaa agagataaag
    41221 gatatacttg catttttaaa gatcatcgtg aatccacagg ataatgaatc tcttacaaga
    41281 gtgttgggat taagagccgg tataggaaag acaactattg ataaattaat tgcaaaagct
    41341 gagaatgaag gtattcagat atttgaatta ctaaagaaaa tttccgctga aaaagatctt
    41401 agttcgaaag taaaaaattt actgattgag attacaaatt atgtttataa atttcagtat
    41461 ctgcaggaag aagttcctct gactgaactc gtcaacagcg ttgtcgatga gattggaata
    41521 ttgagagctt taagactgga aaatactgtc gagtcagaag agcggatcgg gaatattcag
    41581 gaattacttt cagcagttgc ggaattccgt gatactgatg atgagcctac acttgaaatg
    41641 ttcctgcaga aagtttctct cgtagctgat attgacaatc ttgataatca gaaaaatgcg
    41701 gttactctga tgactatcca ttccgctaaa ggtctggaat ttcctgtggt atttatcacc
    41761 ggactggaag aaggtctgtt ccctgtcagc ggagcgaata tgtatgagga tgagcttgag
    41821 gaagaacggc gtctgtttta cgttgctatt acaagagctg agacaaagct ttacatttcc
    41881 tttgcaaatc aaagataccg cttcggcaca ccttcatttc agatgaagtc gagatttctt
    41941 aaagagattc ctgaagatat ttataacagc atcattactt tcgaaaattt aaaattaaag
    42001 accggttaca gatctggcgg agaaaaaaaa tctgtttcag gttcagctcc ttcagtaaaa
    42061 ttcaatccca ctccaaagaa aaaaaattct tcccttaacg tcgaagatga gttcaatgat
    42121 aaatttcctg atattaaaaa aggtgtcact gtcactcata atacttttgg taaaggcact
    42181 gtactgaata ctgtcggaaa gggaattgat aaaaaagctg aaatatattt tgatgatgtc
    42241 ggattaaaaa aaatcatgct gaaatatgcg aagatgactg tagaagacag ctaatgttta
    42301 atgttaattt ttaattttta atttatatat aatgaactac tggttattaa agactgagcc
    42361 tgataattat tcttatgacg atcttgtcaa agacaagaaa actgtctggg atggagttgc
    42421 caataatgcc gcgctgatct gtataagaaa tacaagaaag ggcgacaaag ctttcatata
    42481 ccacaccggt gctgaaaggc agatagttgg aattgctgaa atagtttccg atccatatcc
    42541 cgatcctaaa gcggataatc cgaaacttgc agtttttgat atcaagcctt taaaaaaatt
    42601 aaacactccc gtaactctcg ctcagatcaa agcaattaaa aagtatctgg aatttccact
    42661 ggtcaaagaa ggcaggaaat ctgttgtcga agttcctaaa gaatactggg atgactttct
    42721 aaaaatgagc gggtcaaaat aaatgcctta ttgtttaaat tcaggttttt tgtttgtcat
    42781 aaataaataa acttcttttt taccgtgtat attttaaatt gtaatcatat taatatttaa
    42841 taaagtaata tgaaaaaaat aatatttaca atattctttt tatcaataat attttcctca
    42901 gaatattctt attcccagtt aaccaaagca accatcgggg ttgacggatt tacatgctcg
    42961 ttatgtgcta aaggagttca gggacagttt gaatctcttg attatgtaaa atctgtcaag
    43021 gctgatctta aaaatacatt attcattctg acctttaaaa gtaaacctgc tattaatatc
    43081 aaagagctta acagtgctgt gaccgacggc ggattttcag ttcgtaatat cagtgtcgtg
    43141 gcaaaaggta ctcttaaagg taacaataca tcaggtttct ggttgtctgc tccgaatatt
    43201 cctgatatta aacttaatga tattgacgaa agctttacag acggagataa aatttctata
    43261 gaaggagttg taaatattaa agacctttct atttccgtga cttcaatcag taaactttga
    43321 tccgtcagaa ttatgatcac atgattagtt gcaactgtta atcttctttt gattctgaat
    43381 tctattttaa cttcatttag tctaaaaatt atttttcaaa acttattaaa aatattatga
    43441 atgcgaaaat tattttttca gttataatat tattttttct ttccggaaat ttatatccac
    43501 aaatggatca tcaaatgtcc ggtaatacta aggttaaaaa agataagaac cccgccgtca
    43561 ttatcccagg tctcggaaat tatcattata aagttacgac agacagtaag aaggcacaac
    43621 agtttttcga tcagggatta attctcgttt atggattcaa tcatcatgaa gccgtcagat
    43681 cttttaaaat ggctgcaaag ctggatcccg atatggctat ggcttattgg ggaattgcat
    43741 acgctctcgg tcccaatatc aacttacctg cagacagttt acaaagaaag caggcttata
    43801 atgcagtgag aaaagcatta aagttatcag taaattcttc aaaaaaagaa aaggatttta
    43861 tttatgcgct ttcaaaaaga tatgctgaag atgtaaataa aactgagcag tctgtaaagg
    43921 acaaagaatt cagctatgca atgaaagatg tcatgacaaa atatcccggc gatctcgacg
    43981 ctgctgctat ctatgcggaa agtcttatga acctgaagcc ctggcagtac tggaatgccg
    44041 acgggacgcc ggcagacgga actgaagaaa ttattcttgt ccttgaaaac gtgatcaaga
    44101 aagatcctaa acatcccggt gcaaatcatt attatatcca tgcagtcgaa gcttcagatg
    44161 atccgggacg tgcacttgca agtgctgaat tgctgaaaga ccttgtacct tcagccggtc
    44221 atcttgttca tatgcctgca catatttttt tcagggtcgg tgactatgaa ggtgcatcca
    44281 ttgcaaatat aaatgctata aattctgatg aggactatat caaaaaatat aaacccgaag
    44341 gtatctatcc ggtaatgtat tacaatcaca atataaattt tctttcagtt tcaagaatga
    44401 tggaaggaaa ttatatcgaa gctataagcg ctgcaaataa aattgcagat aatgtgaaac
    44461 cttatcttaa agatatgccg atgcttgaag gcttttatgc aaatccactt ttgataaata
    44521 tttcttttgc taaatgggat gatataatta atacacaggt tccggatacg tctatgaatc
    44581 tgtattctgc tatatattat tatagtaaag cactggcatt tgcttcaaac gataatcagg
    44641 atcagacacg atactgcctt aatgaatttc tgaatttcaa atcaaaaatt actgaggatg
    44701 ttataattgg tttaacccca gctgctctta tactcaacat gatgcaggat ataatttttg
    44761 ctaatgtcgc attgaatgaa gacagaatag ataatgccat taaacttttt aaagatgccg
    44821 ttgcaaaaga agataaaata aattatgatg aaccgccgga ttattatccg agtgtcagac
    44881 tgatgctcgg tgcagctctt tatcagaatg aagaatttga agaagcagaa aaagtctttc
    44941 gtgatgaact taaaaaatat ccgcacaatg gaagaggact ttttggatta aaggaatctc
    45001 ttctggctca ggaaaaaatt actgaggctg aagaagtgga taaggaattt ataaaggcat
    45061 ggataaatgc tgatgtaatt ttaagtatga cagacctgta aaaatttatg aatgtagaaa
    45121 taaaaaagat aaccgctgcc gggacaagac ctttgagaca aattatttta agacctcatc
    45181 agaatgctga cgaactcgtt tatgaagatg atgaaaatcc tgattcaatt catctcggtg
    45241 catatataaa taatgaactt tgcggaatag cttctttata taaacacaga ttaaaggata
    45301 atgccgaaga agaagcatgg cgactcaggg gaatggcaac ttcggaaagc tgcagaggta
    45361 aagggcttgg ggtaattcta atgaatgaat gtattaaata tgttgaggaa aaaaatggtt
    45421 tgatattttg gtgtaatgca cgcaccactg ccgaaggatt ttatgaaaaa ttcggaatga
    45481 aaagacacgg cggtgtattt actcctgaag gactcggaga gcatgttgtt atgttaatga
    45541 atattaaaaa ataaaattaa atgtctgata taaaaaaact ttccctaaaa ataaaaaatg
    45601 tacacggaga cgaactcaat actgatatac gttttcctgc agatttaaaa gaaaataaaa
    45661 aattaccggt tgtaattctt tgtcatggat ttaaaggctt taaagactgg ggatgttttc
    45721 cttatatgat ggaaagaatt tcgaatgaag gattttgtac ggtcgctttt aattattcct
    45781 ataatggtac aggtgaaaat gaagacgaac aaagtgattt cacacgtctt gatctctttg
    45841 cgaaaaatac tttttcacga gaactcgacg acactggttc tgtcatcgat tatctttatg
    45901 ctaattctga tgaacttaaa attgacacag aaaacattac tatactcgga cattccagag
    45961 gcggaggtat tgccatttta aaaaccgccg aagatgaacg gataaacaag cttgtcgtgc
    46021 tgtcttcagt ctgttccttt gacagataca gtgagatgtt aaaatccaaa tgggaagaag
    46081 cagggttttt tgaagtcctg aatactcgta caaaccagat catgcggatg gattatacac
    46141 ttattgaaga tctggaaaaa aacaaagatc gtctggatat tcagaatgcc gtatcaaaga
    46201 ttggagtgcc tgcgcttatc attcacggta ctcaggacat tacagttgat tactcaaatg
    46261 cagaagatct ttacagcaga agtaacaagg atctttttga acttaaatta attgaaaaca
    46321 ccggtcatac ttttggagct gttcatccgt tcgaaggatc gactccggca cttgaagaag
    46381 taattttgaa ggttattgaa tttataaaat gaaaagcttt ctaaaaatat gaagttaaaa
    46441 aaattaatcc tccctttact tccatttgta ttaataattt tattttccat aaatgctttt
    46501 tcatataatg agtctgaaag ttcagatata aatacaactg atagaactga actggcagcg
    46561 gaatacaaag atgaaaagcc tctaactgaa aaagcagatg aaactgaaca caaagaatct
    46621 gaaagtcacg gagataaatt tgcagagata tatctgatac tttttctgat aataattgga
    46681 gctgtgatcg gcagaagatt tcttgccaaa aaatttaatc agccggaagt acttggtgag
    46741 ctgatgttag gtgtattact tggattgata ttgtatcagt tgaataatcc ggtgatgttt
    46801 ttactaagac atcaggataa agtaaatgaa attgttcaga gcggtgtgtc aggaaatatt
    46861 acgtgggatg aggcaataaa taaaaatcta acgattgaag attatgaaaa aggcgggatc
    46921 ggaaatgaac tttcagagat ccttaccagt cctactttcg caagggataa tttatttgta
    46981 cagatcattt tacttttttc aggatttgga gttttgatat tactttttct tgtagggctt
    47041 gaaacgagct ttgaggaaat gtttgaagta ggaggatcag cgcttgtcgc tgcattgatc
    47101 ggtgtgctgg ctccgcttat acttggatat atttcgacta tgctcctctt cccggatggc
    47161 ggtactaatc tgtacttatt tattggcgct actttatccg ctaccagtat aggaatcaca
    47221 gcgagggttt ttaaagacat gaagagaatg catgtacctg aggcaaagct tgtactcgga
    47281 gctgctgtca ttgacgatgt actcggactc ataattttag ctgttgtcgc agggatcatt
    47341 gaaagcggcg aagtacagat agggaatatt tttctgatca ctctgaaagc tctcatattt
    47401 ctgagtctga cattttttat aggtaaacaa tatttacgga agcagattaa actcgcttca
    47461 cttgttcagg gaaaaaatat aagtattctg tttccgtttg caatgatgct gctgatggct
    47521 tggatcagcg acagtatcgg acttgcaagt atagtcggag ctttcgcagc cgggctgatt
    47581 atcaaagaag aatattttaa tgacattccg ggagatgatg ccaaatccgt gaaaagtgtt
    47641 atcgaaccta tcgaagcgat cttcgctccg gtcttttttg tcatcatggg aatgcaggtc
    47701 gatctttctg tttttctgga tgtcaatatt ctcggagttt ctttactctt aaccattgct
    47761 gctgttattg gaaaagcatt atgcggaatt tttgcaaaag gaatggataa aaaaataatc
    47821 ggaatcggta tgatcccaag aggtgaagtc ggactgatct ttgccagtat aggtaaaagt
    47881 atcggagtac tcgataatgc aatgttctca tctgtcatta ttgtagtcat acttactaca
    47941 ttcattactc cgcctgcact gaaatgggca ttttcaaagt atgataaaaa atcaatcgct
    48001 gcataactat tttaatcaac tattattttg agaaaatttt tttacataat attttttaca
    48061 gtctttgtgc ttacaggcat ttatttactg tataatgaaa ataattcttc cgtagaaact
    48121 acttatgcag aaaattctta cctcggtatt tctgaattta ccgataagtt cattccatct
    48181 gatgactgga tggaaaagca aagagcattt ccgtttgatg aaattccaca tgaagaatat
    48241 ttaaaatctg tagagtatgt taaaaaaaat atgcgatcag aatttcccgg taatacaact
    48301 ggttggaatc ttgccggacc aacgaatata gaaggcagga tcacgaccat tgccatccat
    48361 ccgactaatc cgcagatcgt ttataccggt acagcaaacg gcggtctatg gaaatcaact
    48421 gatttttgcc agagctgggt cagtgtattt gacggacaaa atacttcctc catcggagca
    48481 gtcgcaattg atcctgttaa tcctgatatt atttattgcg gaacaggtga atcaaattct
    48541 ctcagaagtt attatcccgg cacaggtgtt tttaaatcaa ctgatggcgg cagcagctgg
    48601 aatttcatcg gactcgacag ttcattttct attggaaata tttccgtcaa tcctcagaat
    48661 actcaggaag tatatgcagc agcaggcggc tggaccagaa gaaaaaatga acagcgcgga
    48721 atttataaat ccattaatgg cggtcagagc tggcagagaa gtttatatct ttcagattca
    48781 gtcggtgcaa tagacgtaag tgtcgatcca tctaatcctt caaaagtata tgcggcaatg
    48841 tgggaaagac aacgcagaga agattacata aaatacggcg ggagattttc gggaatgtat
    48901 gtgaccgcaa attcaggtgc gaactggcag caggtgcaaa acggatttcc atttaatgat
    48961 aataatctcg gcagaatatc ttttgatatt tctgcttctg atccaaatta tatttatgct
    49021 cttacttcaa attcaaacgg tttttcgaga ggtctctata aaaccactaa cggcggtact
    49081 ttgtggactt taataaattc ctcagttgca ggttcatcaa attatgcatg gttcaacagg
    49141 atctgtaaag tcgatccttc caatcctgat aaactttttt gcggaggtct ctcaatggaa
    49201 ttatcaatta acggcggtgc atcatttggt ttccctccta cctctcatgt cgatcagcat
    49261 gcagcagcat tctctgtttc aaatccaaat tatatagtag tcggtaatga cggcgggatt
    49321 gattattcta ctgacggagg atcgagctgg agtgaaagta caacattacc aattactcag
    49381 ttttatgcag gtgaaataaa tttcaataat cccgatgata ttctcggtgg gactcaggat
    49441 aatggaacta taagagcaac atctaatttt gaaaattgga atttaattta cgggggtgac
    49501 ggattttatt gtcttgtcga ttataatgat cctcagaaag tgtatgcttc ttcccagtca
    49561 ggcggactcg gcagatctgt gaacggcgga aattctttta catcggctac aggcggtctt
    49621 gatcttcagt acacaaactg gatgacacca tatgtaatgg ataaaaatga tccgcagact
    49681 ctttactgcg ggacatataa aattcataaa actacaaacg gtatgcagtc atggactgcg
    49741 atcagtcctg atcttacaaa cggacatact gcaaatctcg gaaccatcac aactgtagac
    49801 gccgcaaagt cagacccaaa tgtcatctac tgcggtactg atgatgcaaa tgtctggaga
    49861 acaactgacg caggaagtaa ctggacaaaa attaattccg gacttcctta cagatggatc
    49921 acgagagtcg cagtacatcc cgactctgca aatgtctgtt atgttactct gtcaggttat
    49981 aaagtagatt cgacaggttc gcatattttc agaacgacca attttggtga tacatggaat
    50041 tccatttccg gtaatcttcc tgatgcaccg gtaaatgatg tcatcatcga tccggaaatt
    50101 tcaaatactc tttacattgc cactgacata aacgtaatgt attccactaa tctcggagca
    50161 cattggaata ttttgtcagc aggtattcct tcaaatgtac catgtcacga tcttacattt
    50221 catgcaccgt caagaacttt agtggtctgg actcacggca gaagtgctta caaaattatt
    50281 ttaccggtta ccggtatttc tcagaatcaa agttctattc cttctgcatt taaattaaat
    50341 caaaattatc cgaatccatt caacccaaag acagttataa attacgagtt acgagttacg
    50401 ggtaatgcga agctattagt gtttgatgtt ttgggaaatg aaatagctgt acttgtaaat
    50461 gaaaagcaag gagcgggaaa atattcagta gagtttgacg gaagtaatta ttcaagcggg
    50521 gtttattttt acaggctgag tgcaggtgaa tttattgaga caaagcaaat ggttttgata
    50581 aaataaattt aatcagtgat tagtttaaaa tttcaagttt agaatttcaa aaagctgctt
    50641 gtttacaatc ttcagattgg aagcataaaa atttgctgtg aagattttaa catttaaata
    50701 aaacaatctc atgttaaaaa caatattgac ctgtttagct gccggtatct taattacatc
    50761 ttgcaaagat tcagaaaata ataccaaaaa tatttctatt gaaaatcaaa atccagtagc
    50821 ttctgtagaa gtgtatggtc agaggtattg tgaaatattt gttgtgaaag gaaatgtaac
    50881 agatctcgaa gctgctgttt acaatactct tggctgcaat gattgtccgc aggaggtatg
    50941 gtcagttatc gatccggaaa agcttgccaa agaattggat gcaaagaaaa taattatgaa
    51001 cggaccaagg gtttttttga tgaataagat cggacaattt aatgaacctc cgcctaagga
    51061 aaaaatagga ggtctggaaa tggtgaaacg tgctgtgctt ccgctgaaga tcaagacatt
    51121 gcttgcagga gaaagcgaac cttacgtcga aaacaaagta aaccgccaga ctcagtatat
    51181 ttttaataaa ggaacagagg tctatctgct tatcaacggt gacattcact acattatgca
    51241 gagctttgca atgatcacag atcctgatct gaaaatagaa gatctgaaaa cgctgaacag
    51301 caaaattaaa ttaccgtccg gatggaaata cgaagttaaa gttttagatg aagaatttgt
    51361 tttagaaacc aaagaaggcg gagaagcttt cgtaattcag gatgatctga aaaacactta
    51421 tcaaaagatt aacaatggta aattataatt tgattatatg aaaaaaaaac tatctgttta
    51481 ttttattctt tttctttcac tgatttttat ttcctgcaac cccatgaaaa aaaatgtgat
    51541 gaaagacagt tgtgatgata tgaaggaagt tgtaatttcc gaaagtaacg ggaaatttgc
    51601 tttagtggta aaggaagaaa tttttcaggc aacttcaaaa tccgataacg gaggcatcag
    51661 aaacataacg ggatatacag agttcagatt atcaagttat gatctaaata ccggtgaatt
    51721 attgaaaaga cttgaactcg gagaaataag agaaaatgaa tgtattttac tcggtatgac
    51781 aaaaagtaaa ttatggtata aaagtattga cagtgaattg ggttttcatg caagggatcc
    51841 cgtaacgctt gatattattg taactgaaaa aatggtaaca gacgcaaatc cttttctgca
    51901 gaataacata agcaaacccg aatggaacag cattcaaaaa ttttacggct tcgataattc
    51961 aattggcatg cctgttatct ccgacaattc cggatatctt tactctatcg atcctgtcac
    52021 tctgaaagct gaaaaaatca catcatccat aatgaatatc cgttatgatg aaaatgtttt
    52081 gacatcttca gtttatctga ataacaatga agtagtttat ttatccggaa atccgagaac
    52141 ccggtttaag tatgtaaata aagattttga tgaaccatcg tttctgaaag gagaaatcat
    52201 taaaagttct actgaaactg aaatgcccga agaatattta ctaagtaatg aattcaaaga
    52261 atccgtttcg aaaaaaaatc ctttaagaat ttcacgtaag accgagcccg aatattcatt
    52321 tgcaaagggt cagattatta ctaatgacaa aagtattttt atattaagcc ggactgatgt
    52381 tactgatcag gcaaaagtcc taatatcaaa agtaaaattt gatgaaaatt attccccatc
    52441 gctgcaatgg gaaacagttc ttgataatat ctttagagat ccggataaag cacttgatca
    52501 atcgggattc gaaatggttt tttcaaaagg aaatcccgat ctgagtacta tgagagttgc
    52561 atacgctgac aataaactta tattcatttt tatgctgaaa gctgtatgcc ttgatataaa
    52621 ttcgggtaaa atactttggt caagagatct ctaattttga atggattttt tactttcagc
    52681 cgtcggaaag ttattatttt aatcaatcaa aaacccgcct cggatacctg cggcggtttg
    52741 gtaagtgtct gaatcacgga ttattcggat ttaagg
//
LOCUS       DAORVQ010000004        49933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_182_length_49933_cov_5.520009, whole genome shotgun sequence.
ACCESSION   DAORVQ010000004 DAORVQ010000000
VERSION     DAORVQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 49933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49933
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182_length_49933_cov_5.520009"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1007)
                     /locus_tag="PLX80_00795"
     CDS             complement(204..1007)
                     /locus_tag="PLX80_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQY19362.1"
                     /translation="MNLNKLYNTVNETINDKNVSRRSAFKGISKLGFGALTAAVPLAI
                     AGSINKAVAGGSGISNNASGSTVVEILNFALTLEYLESRFYQQGLLASGLIPGGDRAV
                     FTQISLHESAHVIGLISAVQSLNGTPVPQPTFDFTAGGVYADVFTNYNTFLTLAQAFE
                     DTGVRAYKGQAGGLISNDPILTVALQIHSVEARHAAEVRRIRGQKGWITGNNGGGAPP
                     VIYSREDNYIQGGVDVRTITTVSPDAISEAFDETLTMTEVLAIAGPFIV"
     gene            complement(1043..1756)
                     /locus_tag="PLX80_00800"
     CDS             complement(1043..1756)
                     /locus_tag="PLX80_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQY19363.1"
                     /translation="MNTREKVNLPQKEKENSILNHPINRKRFLMFSGITALGIGLGIQ
                     GCSEDDIVTTSTNSVDLGAGDVGVLNYAYALEQLEAAFYIQVAASFYSGATSAEIQIL
                     TDIRDHEIAHRDFFKAAIGSAAIPSLEVNFSSIDFTSRNSVLNTAKAFEDLGVSAYNG
                     AGYLIDSAAYLELAGKIVSVEARHAATIRDLLNPRSPDFAGNDVVDAAGLDVQRLPSE
                     VLNIAKTYLVTSVTANNLP"
     gene            complement(2101..4521)
                     /locus_tag="PLX80_00805"
     CDS             complement(2101..4521)
                     /locus_tag="PLX80_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HQY19364.1"
                     /translation="MSLFIFSSNLPAIPSNTNDHIKTDQFGYRPGDKKIAVISNPVTG
                     YNNNSPYTPGNIYQLRKWDDDAVVYSAAITSMYGGSTHLQSGDKVWWFDFSNVTTPGS
                     YYVYDSVNNAASYRFEIGTGVYNEVLKQAMRVFYYQRCGTPKSAPYAGTGWTDTHCHN
                     GTLQDTDCRLYNNSNVFTSKDLSGGWHDAGDYNKYVNFTFEAVTDLLLAYKENPEIWG
                     DDFNIPESGNGIPDILDEIKYELDWLLKMQNSNGSVLSIVGANNTMSTSPPSADIAHR
                     IYGPATTSATFSAASIFALAAIQFENIGQTAFANTLTNAAVNAWNWGVANPNITFYNS
                     GIIGAGEQEVDSYERLIRKMSAAVFLYHLTGNSVYKTFVEGNYTQIHMMQWSYAYPFE
                     CGAQNMLLYYSSLIGVSASVANSVKTVYKNSMKSNNSDNLPAYLNNTDAYRSYISNQN
                     YTWGSNTTKGRQGVMFKNMITYKLDTLSNTDYKNAALGFVNYFHGVNPTAFCYMTNMG
                     NSGAENSINEIYHGWFEDGSALWDRVGTSAYGPPPGFVSGGVNPAYALDYCCPSGCGG
                     SNTLCNTALVTPPLSQPIQKSYRDWNTSWPQNSWTITEPAIYTQAAYIHNLSAFIESP
                     ASVDLKVIVQGLYSQINNKLNLRDTIKLILRNGTSPYLKNDSAISVIDSVTFTGNFNF
                     NSNLTGLFYLVSIHRNSIETWSNTPQLLSSGTSVSFDLTNSSAKAFGNNLIQVNIAPD
                     RFALYSGDVNIDGAVDLADQGLIDNDAFNFNTGYIKTDLNGDGVIDLADNLIAENNAF
                     YFIVKITP"
     gene            complement(4663..5793)
                     /locus_tag="PLX80_00810"
     CDS             complement(4663..5793)
                     /locus_tag="PLX80_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY19365.1"
                     /translation="MSIIKLFIKKEFKQFFRDPKMFVMVLIAPVIQLIFLGYAANRDL
                     NYVDTTVYDMDRSSKSRELIRDLEGSGFFIIKEFNTNYEELQSAIDKGTVLCGIVIPD
                     NFEKNILSKKTADIQIIFDGSDGGKAGIASGYVANILSSFSEKVSLDIQERSGLKISL
                     AGRVTPEIRVWYNPNLTTRNFMLPSIVGLILIIITINMTSLAIVKEREVGTLEQLIVT
                     PIRPYQMIIGKLVPFSLLGFIAIILVLSVMRFWFGIEVKGSLVLLFFSSFIFMLSTLG
                     LGLFVSTISKTQRQAMITSAFLVIMPMIFLSGFTFPIDSMPQAIQYITNIIPLKYFII
                     IIRGIVLKGLGFYDLWKELLVLFLMGVFILAVSSNRFQKKLE"
     gene            complement(5790..6920)
                     /locus_tag="PLX80_00815"
     CDS             complement(5790..6920)
                     /locus_tag="PLX80_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY19366.1"
                     /translation="MFRRILSITVKEFRQIFRDKSSLLIIFLLPVALLVLFGYAITTD
                     VNNVKIGVFDKDNSAMSRDFISSLNASSYFTVTEQIRKDSETNELLNTGKIYTALVIP
                     LNFEKDLQSEKEVKIQYIVDGVDASTGGIVQNYLTAATGQFNNKLTQEELSLKGIKSN
                     VPLNVETQFWYNKSLDSTLFFIPGLMAMILIIISVILTSISLVREKEFSTIEQIRVSP
                     VTPLQLILGKIIPYLILSLLIAASIIIMGYILFGVEVKGSYAVLFLGTVCYLLATLSM
                     GVFVSTVADSQQVAFQISQLISQLPTIILSGFIFPIESMPYLIQLLSNITPSKYFIVI
                     LRNILIKGTDISVFYEQIVYLIIFAGIFISISAIRIKREEVG"
     gene            complement(7079..8029)
                     /locus_tag="PLX80_00820"
     CDS             complement(7079..8029)
                     /locus_tag="PLX80_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19367.1"
                     /translation="MENNKSIEVKNLTKKFGDFTSVNDISFDVYKGEIFGFLGANGAG
                     KSTTIKMLCGILEPTSGDALVSGFSIDKEPDDVKKNIGYMSQKFSLYGDLTIDENLNF
                     FGGIYGLQGEALEKRKAWALKISGLEDRENTLTSNLSVGFKQRLALGAAVMHEPKIVF
                     LDEPTGGVDPISRRKFWDLIHEISEKGTTVFVTTHYLDEAEFCNRIILINAGKLIAAG
                     SPAELKEEYIDSTVLEIDCPDSFKVIEKLAKEDYTQSVSAFGNSIHLILKKKYSKGDK
                     ELEALNDVIADTAGGRDNVRIDVIIPSLEDVFLNLLEKRE"
     gene            complement(8035..8961)
                     /locus_tag="PLX80_00825"
     CDS             complement(8035..8961)
                     /locus_tag="PLX80_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19368.1"
                     /translation="MNESAIEVKNVTKKFEEVTALDDISFKVKKNKIFGLVGPDGAGK
                     TTMIRIMCGLLRADSGEVDFTDLDLGEGKKKSIIKNEIGYLSQKFSLYTDLTVDENIQ
                     FIADIHGVNDFEKRRDELLHFTRLKDFRHFQAGKLSGGMKQKLALACTLIYRPKIIFL
                     DEPTTGVDPVSRREFWVILSGLLKENITILISTPYMDEAERFNYIVMMNNGKVISEGT
                     PAKIKSSLKMAIMEVVCNPVRDAYKILKNIENADIQMFGDRLNIVFKGNPMELEEITL
                     LLKNNNIEIDDIRMKLPSLENVFIELMETDND"
     gene            complement(8969..9958)
                     /locus_tag="PLX80_00830"
     CDS             complement(8969..9958)
                     /locus_tag="PLX80_00830"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY19369.1"
                     /translation="MKKITSKFLISSIFLIIISVSIFSGCEEEVDTNAISGSGNIEAT
                     NIVISSKTPGQIKEIYVKEGDMVKAGDVLFLIDHDLLDIQLRQALAAVEQADAQLKLL
                     QQGARSEDIRIAEDNVSAAEINLKQAETDLQRMKNLVNENAATVKNLEDAELVYDLRE
                     NQLNSAEENLMKVRTIIRREEVESAAANLKRSQANADLIRKNIEDCKVSSPLEGIITK
                     KLAEPGEFVNTGSSILNIADLKVVDLFIYVTEAKLGKIKLGQEADITNDTYPDKKYSG
                     EVIYISPEAEFTPKNIQTKEERTKLVFGIKIRIPNASYELKSGMPADANIILN"
     gene            complement(9989..10603)
                     /locus_tag="PLX80_00835"
     CDS             complement(9989..10603)
                     /locus_tag="PLX80_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY19370.1"
                     /translation="MLKKIKDKKTEDRILESAKKIFYNKGIDGARMQEIADEARINKA
                     MLHYYFRNKEALFDAVFKEATQKIFPQVIEILNMEKPLFEKIEYFTDKYITLLSQNPI
                     IPVFVIREMNNDPDKFLKTFSERIKIKPEIFIRQIREAIKSEEILPVDPVQLFINLIS
                     LCIFPFLARPLISNVFGMNEAGFEKFLNKRKKEIPEFIINSIRI"
     gene            complement(11101..12279)
                     /locus_tag="PLX80_00840"
     CDS             complement(11101..12279)
                     /locus_tag="PLX80_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19371.1"
                     /translation="MKILLTILCIVLITGILSAQTFDEKNGTEITKNSKYLKDLKKLK
                     DLKDPNSTKENTSNKDPDENEEDGTGKVWGYAFGDFFYKAGGDSTISLLDYTNYPKDY
                     NSFDFRRIYLGYDHNISRYFFTRFVLAYDGNDVLPNLKNAVLIKDAFLAWKEIFTGST
                     LTIGLQPTPSYSFIEEKIWGYRSVEKTIMDQRGIAGSRDLGVMLNGNFDEKKNYGYYF
                     MIATGSGVRKEVNKYKKYYGMLSGNFADKKITADIYADFENVNDNNDRYTFSSFLAYS
                     VESFSIGLEGFFQRNKIFNSVTNNTDPMGLSLFATGNISENKFRFFSRIDYYNENTTT
                     AQRGFNQYFVTAGIDFMPHKNVHLMPNIWLNAYSQKNSSAAGLKTDVVPRITFYYDYR
                     "
     gene            12688..13533
                     /locus_tag="PLX80_00845"
     CDS             12688..13533
                     /locus_tag="PLX80_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY19372.1"
                     /translation="MKNILFPTDFSQNADNALNLAIDIAKKTNGTLILFHAYSVQLVD
                     PNMPAEIYLSAYQEEEKSAKENLEELRKKIMDFNKDDTGKNLFEVEAIVSQGLVVDEA
                     LSIIDEFNIDIVIMGTHGASGLTELILGSNTASLIENSPVPVLAIPQNVKNKSIDNIV
                     YAYDDIKANMPSFQRLLNFSEIFNSEITLLHIIETGKDTEEKNKKAFEEIKNNIGSDK
                     IKLALVTEENVLEGINEYINSNDVDVLAMAIRKRSLLDKIFSRSLTKKMAYHTKIPLL
                     ALHKV"
     gene            13603..16500
                     /gene="polA"
                     /locus_tag="PLX80_00850"
     CDS             13603..16500
                     /gene="polA"
                     /locus_tag="PLX80_00850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQY19373.1"
                     /translation="MAEKLFLIDAMAVIYRAYFAMISNPLINSKGRNTSAVFGFVNSL
                     LKILEEQSPDHIAVCFDTAAPTFRHKEFPAYKAQRDVIPSDMPWQIEKVKEVIKAFNI
                     PMIELLGYEADDIIGTLVKQAEKEDVISYMVTPDKDYMQLVSDKVFMFKPARTQYGTD
                     NEIIDSEGVIKKFGVPPDKVIEVLGLMGDASDNIPGIKGIGEKTAAKLIQEFGSIENM
                     YNNIDKITKPKLKENIESQKNEALLSYRLVTIKTDVPLDINFHNLNKKPEDAPLLTAL
                     FEDLEFKTLTRKFSGDQSDTDAEIKGSAKKAASAKSIPDFAKVDITGPDKVLKTIKDV
                     KHDYIIIKTEKEFEELIKLLSKEDIICFDTETDALDAMNTNLVGMSFAFEEFKAYYVP
                     VTGKMFDKNFKKGSKAEKEISLFDTSNNVDNKISKNEADKGIDISTAIKMVKPLLENK
                     KIKYVGQNLKYDYLVMRNYGIELSDMFFDTLIGSYILRPEGAHNMDALSEKFLGYKPI
                     SIKELIGKGKDQISMDKVDIDIAGEYAAEDADVTLQLYYILKYELGKLDMRKLCDVVE
                     FPLIKVLSEMEFEGFKVDERILNTIDKQTQKFIIEYEAKIYAAAGEEFNINSTQQLAE
                     ILFNKLNLTPTKKTKTGFSTDVKVLEDLKYQHEIASLLIDYRMLTKLKSTYLDGLKKA
                     VNPRTGRVHTVFNQVAAATGRLSSVDPNLQNIPIRTEAGRSLRKAFVPRDNSYVIMSA
                     DYSQIELRIMAHYANDENMLNAFKRHHDIHTETSMRIFGLKSKTEVTAGMRRKAKEVN
                     FGIIYGIGAFGLASRLEIKNSEAKEIIERYFREYPNVRTYMEETKKFAHENGYVETLL
                     GRRRYLPQINNQNAAARAEDERAAINMPIQGTAADMIKIAMINIYVEFQKNKLESKML
                     LQVHDELVFEVKKTELEKVKKIVTDKMKNAIALNVNIEVEVGEGDSWYEAH"
     gene            16606..17622
                     /gene="rfaD"
                     /locus_tag="PLX80_00855"
     CDS             16606..17622
                     /gene="rfaD"
                     /locus_tag="PLX80_00855"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQY19374.1"
                     /translation="MIVVTGGAGFIGSALVWRLNQLGEEDIIIVDDFTKDEKESGDKS
                     FSAKDDKWKNLVHLKYADVFDKEDFGHMLSSDFMKNFEVETVFHLGANSSTTERDFGS
                     LMTNNYEYTKYLCEKAIENNVRFIYASSAATYGDGSKGYEDDESKLNTLVPLNGYGYS
                     KHMFDIWAKKLKLLDKIVGLKYFNVYGPNEYHKEDMRSVVNKCYHQIKETGKARLFKS
                     ANPEFADGGQSRDFLYVKDAIEMTLFFRENKIVNGIYNIGTGESNSFNEFVKPVFKAL
                     DIKENIEYFDMPEILQEKYQYYTKADITKLRNAGYKKEIYKIDDAVTDYVAGYLNTED
                     AYLN"
     gene            17730..19451
                     /locus_tag="PLX80_00860"
     CDS             17730..19451
                     /locus_tag="PLX80_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQY19375.1"
                     /translation="MKSILLILLFVTLTGKSFSQDLPHYMTDKEKELSKNYQYNFFPT
                     DNTNPPPTPVRTMAEWEEVQGVIVAWTSYTTIIRQIVDYVQDECQVFIVCSDSNSVKT
                     FLTSGGVPLVNLRFIVAPFNSVWCRDYGPWAAYSGISDSLKIIDWIYNRPRAQDDLVP
                     TAFANYTNLPIYEATVAPNNLIATGGNLMVDGNGTAFSSKLIIDENPTKTESQIDGIM
                     NSYLGVSRYIKMNTLPYDEIHHIDMHIKLLDEETLLVGQYPPGVADGPQIEANLQYVL
                     NNFQTCYGRPYKVVRIPMPPSSGGQYPPSSNYFTYTNSVFVNKTIIVPIYGLAQDSTA
                     LRIYRENLPGYRVVGLNASQMISALGAIHCITKEIGVAEPIFFSHPKLHNTLNTTSPY
                     EVKSYIKTKSGVASAKVFWRTDTTQSYNEITMTGSLDTFRANIPQQNLGTNVYYYISA
                     TSNSNRTVTKPITAPQGYLKFVIDNPTSVISNNSQAENYSLSQNYPNPFNPKTVINYK
                     LQVTGNAKIMVYDVLGNEVATLVNEKQNAGSYSVEFDGSNFSSGIYFYRISSGNFNET
                     KRMMLIK"
     gene            complement(19649..21892)
                     /gene="rnr"
                     /locus_tag="PLX80_00865"
     CDS             complement(19649..21892)
                     /gene="rnr"
                     /locus_tag="PLX80_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQY19376.1"
                     /translation="MNTQEKILELLNKNKDTSFKVNSILKKLNLKPSQRETLKKHLHK
                     LVNEGSIQRDRKYYSLNTGNKLAEPIVKKSSDKKSGSKNLRFKTDSSNKGRLKVSGYK
                     FEKTADINKEDITGEIDFGKDGRPVIKVKSESDEIKIYNIKSSRTYAHKIGDIVKFNS
                     LKSDEGNDAEIIEIVTHIKKFVTGKFEDHKSYGLIFPDSREIKKEIIVSFRDFNEAVH
                     NDKVYAEILNPGDHGDEFTDLRAKVLEVLGKSGVRGAEEVSIMRKFGLVKEFPNEVMK
                     EAEVLKIEYDPKDRLDLRDKVIFTIDPEDAKDFDDAVSVEKIKGGGYILGVHIADVSH
                     FVQEDTELDKEALRRATSAYLVKNVIPMLPEKISNELCSLKPNEDRLTFSILITLGKS
                     LKVKSFEIRKTVINSKRRFTYEEAQKIIETGKGDFYKELKVMDKLSKSITLERLREES
                     LDFDSNEVKFKFDKSGSVAEIVVKERLNSMRLIEEFMLLANKCATEFVGNISKEQKIN
                     YPFIYRVHDTPNPDKMKELSEFVKQFGYKINVEDKESLRQLLNDIEGKHEEFIINDLF
                     IRSMAKAIYQTKNIGHYGLGFKDYSHFTSPIRRYPDLAVHRCLFDYLKGGKKLRERIL
                     HYEKVLPEICKQSSIMEQNAEKAERESIKLMQTDYISKHLGSEYDGIISGMMQYGIFV
                     EIMDILVEGMIRFRDMEDDYYVYDEKRHMAFGRRKKKTLKAGQKVRIKVIRVNKESKK
                     IDFALVS"
     gene            complement(21897..26570)
                     /locus_tag="PLX80_00870"
     CDS             complement(21897..26570)
                     /locus_tag="PLX80_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19377.1"
                     /translation="MSKEENNPDITDSSDNKENSEIQNIAEQTPKKKSVIRKIFSVFK
                     FVIIFFVVLIIGIFIFIQTDMFSNLALDIALDKLNESLAEKDAVIHAESLNGNILKGF
                     TLNNGSIKIKGDTLLKFNSLSAKYNIIKLLSKEIFARELILREPQINVTKIRDNNDSL
                     KWNFEYFLESEDKEEDTTTSEFDWGITAENFTIENGFIRILENKNSDKPIREIKMKQI
                     DTFNVSEFDLSELNLNLSAKYFPDEKDVNISKLNFKTNSDFNLNQLSLKANINVKDTI
                     TDISKLNLITDRSNILIDKIRLDDLNALEGIEYEEFEKKDAKINIQANPINADDVSFF
                     LPQLDFIDSTISFHLIAEDAYGDLKLKKFDITTEKSEFHFTGNLKNLHIPESLYINVS
                     GKDISIDPRDTRIMVPGLPIPDYSHLGVVTIPELRYTGKPDNFETDFDIISSAGKTYG
                     NMKMDLRGNEIVYKGDIKTSAVNLGKIFKEKDLEGSISGEFIIEAKGFDYKKNSGSLK
                     YRLVSTKFYGLNIAASEGKILFNRGNLNLDVSIQSNLVTGKTKGKLNISNPKNLSYDL
                     TGTVSNLDLQVITKDPEMKSNLTFDFDVNGKGISPDDIAGSFKIDLKPSEFGGFNLPS
                     TPILADIDQNGNIRKISLKTDFADVEADGYFQFTKLLDLVIENSEKISNSITDKFFPD
                     TSGIQPVITESGYVIDCNNYYMNYTVNVRDLEPIYSLIGDTIIFNGDLKGSISDSCGL
                     FTFNTNGKISNFAYGDSLFILKDANLNLFMRNDLNTNDMNKFYTDITLHSNKVIAAKN
                     KFDSVFTKINFFNNNNQVFLTANRDTTIKLYTNLSLKDSALFVFDSLSVKYHDMNLAN
                     NSDLIIKYTKVDSSQIFDFRKFVVNSLNQKVTVNGFYSLNDSSNIKVSADNIKVYTIL
                     KLIKPGEDFDTVDVIKGNLRRTLLTYKGMPDNIDLSLETNSDVLAIGQTKIGRLDAFV
                     DYKDEVIKSDVAFYNINNAGNFKLTGTFPFLNKAKDEYNDTVVYNKKRSESDVNISAK
                     ADNFQLKIFQQLLPYTKDLEGILDGEIKLLGITSDPQLTGDMNLNKGKFYVTLTKMRY
                     DFDAGISTGNNKILINKSKIFVPEDPTKFIAITGYVDLTDLKMNDIDLNMSGDVKAFD
                     KDNGPTELGISGDLWIGSGSPSLKIKGNSDRIDLTGNLTLIRGNIVFNPFVQEAYNIY
                     SDDFEYGVIIDSLNYNNEFVKRVLKNSSDSVVIIKGLNLNPFQKVLYTDNNKNIKYQQ
                     REKSGLFFYDLLVTTSGNVFLKFLVNEKSQQEFFGEIKTRLQVDNKENYQMSGRGEVT
                     LGNNCYYKFFRKFDATGKVIFNGPLTNPMLDIDAKYKGYTSVDIGGSGEQYINEVVID
                     MNVTGEAKNPVLTMNLERNGTRESGSNATSDAISFLLFGKFKDQLSFSESSSFGASLG
                     ASVLSNYVTNSLETVLPFLINTDVNYIDSKTGSIAENTDIRFTAAIGGAVIRFGGQIF
                     KGIANTDIIIDYPVSKMLNLKSLSDDLVLRVERVYDPFSSASTDASVVSGTRAGALIY
                     YRIKF"
     gene            complement(26604..27068)
                     /gene="rfaE2"
                     /locus_tag="PLX80_00875"
     CDS             complement(26604..27068)
                     /gene="rfaE2"
                     /locus_tag="PLX80_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQY19378.1"
                     /translation="MILNINNISDVRSRIKSENKKLVFTNGCFDIIHKGHVSYLNQAK
                     LMGDYLIVGLNSDKSVKKLKGDDRPVNNENDRAFILDNLKSVDFVIIFNEDTPYELIK
                     LIVPDYLVKGGDWRKIDIVGSDIVTAAGGKVLSLNFINNYSSTGIIEKIRKH"
     gene            complement(27065..28060)
                     /locus_tag="PLX80_00880"
     CDS             complement(27065..28060)
                     /locus_tag="PLX80_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HQY19379.1"
                     /translation="MNKKLLTGIFENSRDKKIIIIGDLMLDRYLFGDVTRISPEAPVQ
                     VFDIRKSEFRLGGAANVAYNIKTLGSEPFLIGITGEDSESDLLKNSMQDLGIDTEGII
                     PEKDRPTTSKSRVISDSHHLLRIDSESKDDISDNSQTKILSLLSEHAEKDSIVILQDY
                     NKGVLTEKLIRKVSALSLKMNFKILVDPKFDNFFEFKNAFLFKPNRKELEDSFGIKLR
                     DLNDLDDIAAKLIKKISCQNVIFTLGEQGMMIYENIRGKLKKEKISTKARKVADVSGA
                     GDTVISTIAVCLSGGASLRDSAVISNFAAGIVVQEVGIVPVNKKELIENIIKSGK"
     gene            28249..28842
                     /locus_tag="PLX80_00885"
     CDS             28249..28842
                     /locus_tag="PLX80_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19380.1"
                     /translation="MIVSTFIYLFFIILSSSDHQNSTEIKDLYNTGEISKGVVLIDGE
                     TDPHYKLIKSSDNGYPGPDCKVSLSNGFPMDRWVPNSNRSKWIAPRTDAFKFNEPGIY
                     VYRTEFDLSEFESENIVISGVWSTDNNGVDILINGNSTGFATPIEAYYGMFPFEIKNG
                     FVKGINTLDFLVYNISAPTGLRVELQGRGVKKIITSR"
     assembly_gap    28868..28967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29072..31888)
                     /locus_tag="PLX80_00890"
     CDS             complement(29072..31888)
                     /locus_tag="PLX80_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19381.1"
                     /translation="MHTLLVLFVILFMAFQTTQATAEKKINSSEQSQTVSKENPYAEN
                     VHMYICMQALQLLKDKYPNFDYTKFDQHIGDMTDEGTRAWQTTKITTGAYREDKEDVV
                     YDVRGPGNLYVSNTHFWNADDRTNGDNTLTYLNSIGHYPNAYTKLNKFRDGQWFEWTG
                     DSGEREYINYLHHSGFVYRYSYHTRGLINFFRTKKIWLEDVYDLLGRQTLVRQEITAP
                     ENVFNAVVWEVLGRMSHLLQDLSVPAHAHNDVHVRELDGGDCYHNFIDDGAYLNYNWQ
                     TAKNAGGFINPYADNSDPLRYLAYTTSQLADHFPSGPSCSQIPQQHLGNNFLPGGTNT
                     MIQNYYQILGSPPLNIPDLNMEANYCFNHSIRAVAGLFYWFGVETGLFPTETNMRPNI
                     TGFINSSPDNRLYRGETMNIECIASGNPQNFDFEILVCDTANLCLLPVQGISLSQNQN
                     IFSMTNINFQNYWTCLRYDSVCSSSAGSFSAEPPLHFTIKVTASNQFGSDTKFYGSGN
                     LQWINPNQFLRPNPPYSGCPFVLVNNGEKFVYDNNLLHRSEHLINAGKYIEDKYKLKT
                     TPSVYPQDSTIMLAVKETNIDVNYFDQFRLLIADHPVGTEMGITENNDPVIYTPKYVI
                     SPSHAEISGKNITNYLKYGIQNPKFIKGIENEILSAKFPEASSLPSESDSIALIFNPG
                     SAGLTPVIPVVKDIAGNINIQDSKGNNEKSDLIFSKRIVNSEIILPVFANKGIDALTI
                     EWKDNFQMSYLAYTGINYSGFFTEELELVEAEDLFTGNVLNFVKDKDNITIQCDSSNQ
                     LILKFKNSHSPLKYGYLRSYIFITNGRYEKGKQFNIENSISENKLKADNSKIKSTEFT
                     VGNFPNPFNPKTIINYQLPKSDVVSLKIFDVLGNEVMVLVNEKQNAGTYSVEFDGSRF
                     ASGIYFYTIRTGDFIQTKKMMLIK"
     gene            complement(32540..34537)
                     /locus_tag="PLX80_00895"
     CDS             complement(32540..34537)
                     /locus_tag="PLX80_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HQY19382.1"
                     /translation="MHHFFIIFIFLLSVNFAKGDIIYTEPVRNAKLVSINNNIIIGFD
                     EIIQSSDLLKTIIVKGSVSGIHSGKVILTKDKRKFLFIPSEPFAFNEEVEVKLIRLKT
                     LSGFINNFSYIFYTQKSKPLDVPNQSILNETGNSDLIYNSQMQGSPDLPNISVRILNN
                     PSAGKLFLGNFPFSQIPNTPYLLQSDNNGNISYFREVYVWALDYKRQPNGMQTFFEGL
                     GYHAEDNDHNYIRSYFCGNGYSTDHHELQITNDGHAFLMSYDPQHVDMSMIVPGGNPN
                     AIVTGLVIQEIDESNNVVFQWRSWDHMLITDAIGINLTDSLIDYVHGNAIDIDDDGHL
                     LISSRHISEITKINRNTGDIIWRMGGKNNEFTFINDPIKFSYQHDIRRLDNGNVTLFD
                     NGNYHSPPFSRAVEYDLDEVNKTATLVWQYRNTPDNFGFAMGSVQRLKNGNTLIGWGS
                     SHPNISEVTPEGNIALEMSLPAGIFTYRVFRDEVKFTLNLKMAVEGFYNIDEDKLKIN
                     DTVMIYLRDINSPYSIVDSSESVIDSVNLTGSFNFYNVNAGTYYLKIKHRNSIETWSK
                     TGGENLKENDVLHYDLTSQHSQAFGNNLTLTGSRYSIYSGDVNQDGTIDISDLSDIDN
                     DVSVFNKGYVNSDITGDMFTDVSDVAIAENNAVKFISSVKP"
     gene            35041..36342
                     /locus_tag="PLX80_00900"
                     /pseudo
     CDS             35041..36342
                     /locus_tag="PLX80_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            36388..37224
                     /locus_tag="PLX80_00905"
     CDS             36388..37224
                     /locus_tag="PLX80_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19383.1"
                     /translation="MINYTDNYFDLEILKMSRIKLHEATESIRLRNIFNRISRSKYLI
                     NPVITGRYNDDIILLDGANRYGSLKEIGCKLILAQVLDYKSPDLQLDKWNHLVYDLNI
                     NEIINFCEKNGFKFERTSYSKGIKKIKNDFHYTLVCDIEKDENIIIELSKDLGEMMVQ
                     LHNLTTFYFKNYDFDRSEGDIKFSDIKKYTRRNGVLIVFPDFKKDHILKIADSKFHLP
                     AGISRHRIINRVLHVKYEIRKLLSDKNIAQKQKELKELLNDKIDKNKVRQYQESVIVF
                     DE"
     gene            37261..38487
                     /locus_tag="PLX80_00910"
     CDS             37261..38487
                     /locus_tag="PLX80_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQY19384.1"
                     /translation="MKPEYFIARRYLFSKKKFNFITIISLVSIIGVCIGVAALIIVLS
                     VFNGFNSKVTNILVSFDPHIRVESADSSKLTDYSEVQKKIQELGIKSATPYTVNKGML
                     ANEEMNEVVFIKGVKENEIAEVSGIKDMIKLGEFDLTNKGEAGGIVMGFSLLGKMRAR
                     PGDTLTLLSPVGLENALTQFVEPKTKKLIITGVYDSDNKDYDGSYAYISLENSQEIFD
                     LGNRVNGVEMRLSNINDSEPEKIKIQNALGNNFNVLTWYDLHKDFYSILKVERWVAFT
                     ILSIIIAVASFNILGSLTMTVIEKKRDIGVLKSMGATNKMITNIFITEGLSVGIIGTV
                     TGMILGLGIVIAQIYFEFYKLDTSVYKMEALPVELRFMDLVYIPIAALLLCYLASIYP
                     SKRAARLNPVNSIRWE"
     gene            38542..39222
                     /locus_tag="PLX80_00915"
     CDS             38542..39222
                     /locus_tag="PLX80_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19385.1"
                     /translation="MTTEDPKVLISIEDLNKSYPLAGTKKLDVLKGINLNIYKEEICA
                     ITGKSGAGKSTLLHILGTLDQPDSGKVLFEGENIFTKKEKQLAEFRSSKIGFIFQFHH
                     LLPEFTALENVMIATMINGKDDAGKSFEILKEVGLGERADHKPSELSGGEAQRVAIAR
                     ALVNSPGLILADEPTGNLDTENADSIMNLIFDLREKFKQTFVIVTHNEDFASKCDRII
                     KMRDGKIL"
     gene            39247..40005
                     /gene="pstB"
                     /locus_tag="PLX80_00920"
     CDS             39247..40005
                     /gene="pstB"
                     /locus_tag="PLX80_00920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQY19386.1"
                     /translation="MDGAKIVTEKLNLYYGTNRALKDVSIEIGDNQITALIGPSGCGK
                     STFLRTLNRLNDTIDNVKLEGLVKIDGIDIYDKRVDVVSLRRRVGMIFQKSNPFPKSV
                     YENIAYGPRINGVTNKKELDDIVESNCVRAAIWDEVKDRLKDSAIGLSGGQQQRVCIA
                     RALAVNPEVLLMDEPASALDPISTAKIEELMFDLKKNLTIVIVTHNMQQAARVSDKCA
                     FFFTGEIVEYTKTSSMFTKPEKEQTQNYISGRFG"
     gene            40081..40761
                     /gene="phoU"
                     /locus_tag="PLX80_00925"
     CDS             40081..40761
                     /gene="phoU"
                     /locus_tag="PLX80_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQY19387.1"
                     /translation="MLHYLEEELDRLKEKIIKMGSLVEEQIDFSFKALFNGDLELAQT
                     VIDRDDEVDKFDLKIDKHCQRIFALTQPVAFDLRLIMSSLMINSDLERMGDISVNVAE
                     RVKVLKGHAELLRKMRVNEMAEKVRKIVKMSIDCFVNGDAELAKSVINFDEEIDNLDR
                     LIFNLLTEEMKINNNIIVPCCHILSLVTNIERLSDHATNIAEDVIFLIDAKMVKHSKD
                     PENLKPKE"
     gene            40771..42678
                     /locus_tag="PLX80_00930"
     CDS             40771..42678
                     /locus_tag="PLX80_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQY19388.1"
                     /translation="MKYLITLLFFISFVNVHSQTIKSDSVKKIPYDHNRILNPTELSL
                     QSDIFSYDSLYIWSDKRTLSEIMDQRPGFFINNFGLGSRNNLNYNGNSYYQTGVFRDG
                     IQVNDNYYQGFDIQNFSISEIDKVEEISAVSSFFYGINSSSKSVNVITKDIFNPQPFS
                     QFRYSQDRDGSLYADVFLTLPVSRKGNVMIGLTKHSLDGLYANSAFDVWRGRARVNLF
                     LSPKVNLRLDFNMSNFDRGLNEGLIYSPVKDSLQNPMEAMVVDPNQTENLENYFYSAQ
                     LTAKLFKNQDWLTKIKLYSVNSLRTLYNERDTSFVNNYNLDSGYQHSILYGTELLQNI
                     TLNSGRHFNSDLTFGGNVYGNLFNGNFNSVIDNYFRNNSQYLYFSLKAKYYLNYKIFS
                     ATTLIRNDNFSEYGFLNYGAEGSIKAFDKNNFLLNFNGGYRRTEYELFNAAKPKLLSF
                     EEPPVSYTVNNFYNAGLSFGYKQSELYFEYSSGKNQYANNSIQNINAGINLISEYFDV
                     MVNYNNSNDLLVPENYIKSDLAFKDILFRGKLKLRTGFNFKYYNIRYITDQYQSVYSY
                     GVTNNTFPQQNQYMVDFYVGARIGRANINITVANILNTLVYNAYLFPLDDRGGLFNAI
                     SRFTIVWDFIN"
     gene            43250..45247
                     /locus_tag="PLX80_00935"
     CDS             43250..45247
                     /locus_tag="PLX80_00935"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HQY19389.1"
                     /translation="MKYILIITISLLSFSFAKTEIIYNDPLPNAQLVSINNNIVIGFD
                     EIILSSDLIKNISVKGSASGLHSGNIILTKDSKKFLFIPDEPFAFNEKVEVKINRLRT
                     SSGKLNNYSYSFYTQKSKPEYNPDQSFLSETGNSNVIFPSQNSGSSNLPVLTVSINNN
                     PSPGKLFLGNFPFNAIPNTPYMIQSGNNGNISYSREFSSNVLDYKRQPNGMMTYFEGN
                     AYYGEDNDHNLLRTFQCGNGYSTDHHELKVTNDGHAFLMSYDPQQVDMSKVVPGGNPN
                     AVVIGLIIQEIDESNNVVFQWRSWDHFLITDAVGVNLHALTIDYVHGNAIDIDNDGHL
                     LISSRHFSEITKINRNTGDIIWRLGGINNEFTFTNDSIKFSYQHDIQRIDNGNLTLFD
                     NGNFHSPPFSRAVEYQIDEVNKTATLVWQYRQTPDKFATAMGSVQRLKNGNTLINWGS
                     SNPNIFEVTPDGNIVLRMSLPTAIFTYRVFRDEVKFTLNLKMAVEGSYNNNQDKLNIN
                     DTVTAYLRELNSPYNIVDSSRSEIDSVDLKGSFNFYNVNTGSYYIKIKHRNSIEIWSK
                     SGGENINETDVLHYDLTSSQSQAFGNNLTLKGSRYCIFSGDVNQDRTIDIFDLSEIDN
                     DVSVFNSGYINSDITGDLITDLNDVAIAENNAFHFISSVVP"
     gene            complement(45340..45495)
                     /locus_tag="PLX80_00940"
     CDS             complement(45340..45495)
                     /locus_tag="PLX80_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19390.1"
                     /translation="MVKIKLKGKDPLVINGSITGRTYIFKSTGDINWVDRRDAGDFEF
                     IKDIITI"
     gene            complement(45567..45878)
                     /locus_tag="PLX80_00945"
     CDS             complement(45567..45878)
                     /locus_tag="PLX80_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HQY19391.1"
                     /translation="MQLIKTGEMPDIVKYFVIIIVVWRITHLFSMEDGPFDLIFRIRK
                     LLGSGFFGKLMDCFYCLSIWVSLLLSIIIGIGFAEIIILCLYYSGAAIILEKFTNKNF
                     E"
     gene            complement(46005..48104)
                     /locus_tag="PLX80_00950"
     CDS             complement(46005..48104)
                     /locus_tag="PLX80_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19392.1"
                     /translation="MAKKLKTSKTGKARLTIQPERSNFLMMLAKNPNYFGNIPESKIK
                     SNFKMIGNLSYEEISCIGYNPSTQILEATFNIKRSYGYSGNLCTAGSTEYVRFYINFH
                     DGAGWKDQGVTAVNVHDIPADTDCNKKSTMPLTYTASLKKKTSKYSKCSQPVLPRVRA
                     ILSWNIEPPADSPSWNPVWGNVKEADIQLKPYFLFYDDLSILKYSELFNLAALTPDIS
                     LKQLAEVSEINIDDLKPVAKTLSLSELNSKYKKADIDPSRFAYKAAINMIKYPMSELT
                     LNSQKVLKDLKIDYTKLIDKFSAFPLKDKSKANVEWEELECIGLDYNTESLVATIKIK
                     KKSGYSGDLCDDGSKEYVAFWIDWYDDCKWEYLNTVELDIHDLDIPAGGLFYSVTLPL
                     DTTLHKKICENPNIVRVRGVLSWNALPSVTDPDDLNFYGNREDTHVQIKPGTEIGSVM
                     PLYNIIGGIPVDKINDVTGLTTPGASFAFNGLPVPSGSPFGGVVVVNGPTFPGERYRI
                     KVRNTVTNVSSYLSNSFTVVGWLPVAPYVQYTTQTPDAFGYYNFLTVNQNTLSILARF
                     NPGTNDRLEVSLEIESVFGTFSKNIKMDNIAPEIALSVDDGGDCTHYHKGDTITGHYY
                     VHDANIHTFSFGSSFGGGVSGTSNTLPLPGNSFSIPTNASSNPCGNISLYASDKTIVN
                     SQSVGFHVWTGYNICLQ"
     gene            complement(48502..49554)
                     /locus_tag="PLX80_00955"
     CDS             complement(48502..49554)
                     /locus_tag="PLX80_00955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY19393.1"
                     /translation="MKILINALSGNGDALMFSPALKILKDKFPDAKIDMLVMFRSVKE
                     MYAESPYLSEIHFIDLMKQSKIKSISEIRKLSKNEFDFSINVYPANRLEYNVVNAFLG
                     AKKKIAHHYLHTTLSRAEFMNDILVNEIKDRHNVLQNLDLIKSILSEVKDDDAGPMEI
                     FISEGHKSAAEKWISENNFEGKMLIGFHPGSSVLKNHIHKRWDKDKYVKLGKELISEH
                     NSEILLFGNEFDLNNEIKDKIGSHSHIASTSDYMDSVARIKHCNLFISNDTAFMHTAA
                     ALQLPVVGIFAYTNYKELYPWKTEYRIVRKDLECSPCFYNSPKPAACIWTGEDEFKCM
                     KNISVEEVLKAANKLI"
BASE COUNT        16303 a         8585 c         8220 g        16725 t
ORIGIN      
        1 ttccatctta cattactctg ataagtagtg atttgaaagt tagaaacatt atttaaatta
       61 tttataaaaa atttgtaacc tcagtctata caaataatta ttaaatttta tgttttatgc
      121 aaaaaaaccc tgcatatatt taaataacgc agggtttttg gaaattgtaa aatggaaaaa
      181 ttgaaaattt acttattact aaactaaaca ataaacggac ctgcaattgc caatacttcc
      241 gtcattgtca gagtttcatc aaaagcttct gatatagcat cgggacttac ggttgtaata
      301 gttctgacat ccacaccacc ctgaatataa ttatcttctc ttgaataaat aactggagga
      361 gctccacccc cgttgtttcc cgtgatccaa cctttttgtc ctcggattct tctgacttct
      421 gcagcatgtc ttgcttctac tgaatgaatt tgcagagcaa ctgttaaaat tggatcatta
      481 cttatcaaac ctcctgcctg tcctttataa gctctgacac cagtatcttc aaatgcctga
      541 gccagtgtta ggaaagtatt ataatttgta aatacatctg cataaacacc acctgcagta
      601 aaatcaaaag taggttgagg aaccggtgtg ccgttgagag attgaactgc tgatattaaa
      661 cctatgacgt gtgcagattc atgaagactg atttgtgtaa agactgccct gtcacctcca
      721 ggaattaatc ccgatgctaa tagaccctgt tgataaaatc ttgactcaag atattctaat
      781 gttaaagcaa aattaagaat ttcaacaact gttgagcctg aagcattgtt actgataccg
      841 gagccgccag caacagcttt atttatacta cctgcaatgg ctagaggaac tgcagctgtt
      901 aatgcaccaa atccgagttt ggatattcct ttgaatgctg atcttcggga aacattttta
      961 tcattaatag tttcgttaac tgtattatat aatttattta gattcatctt atattttctc
     1021 cttatggttt taaattaata gtttatggta aattattagc agtaactgat gtcactagat
     1081 aagtcttagc aatattaaga acttcacttg gtagtctttg tacatcgaga cccgcagcat
     1141 cgactacatc atttccggca aaatcaggag atcttggatt tagtaagtcc ctgatagtag
     1201 cagcatgtct tgcttctacc gaaactattt ttcctgcaag ttcaagataa gcagcagaat
     1261 caataagata tccagcacca ttataagctg aaactcctaa gtcttcaaat gcttttgcag
     1321 tattcaagac actatttctg ctggtaaaat caattgaaga aaaatttact tcgagtgaag
     1381 gtattgcagc actgccaatc gccgccttga aaaaatcacg gtgtgcaatt tcatgatctc
     1441 taatatctgt taagatttga atctctgcag atgttgctcc tgaataaaat gatgctgcaa
     1501 cctgtatata aaatgcagct tccaattgct ctaaagcata tgcataattc agaactccaa
     1561 catcacctgc acctaaatct actgaatttg tcgatgtagt aactatatca tcttctgaac
     1621 aaccctgtat gccaagtcct atacctaaag cagttattcc tgaaaacatc agaaatcttt
     1681 ttctgttgat gggatggttt aggattgaat tttccttttc tttttgaggt aaatttactt
     1741 tttctcttgt attcattttt ttttaattta agttgttact ataaacaacg ttttaaaatc
     1801 ctgattggat ttaattaatt aaaaatgtta aaatatatta gattcggatc tataagatta
     1861 tttttttcga aattatcaga cttctaaaaa agttaaatga tttttagtga ggagaattga
     1921 ttggaggtta ttagtacttt tataatctca tttatttaag ttacttataa attgtgctgt
     1981 tgatttcaga ttttggtaac ctgaaacttt ttactgaatt ataaaatatt ttcaaaaata
     2041 cctggcaaaa cttttataag tcaatagtgc tcaattctat ttttaaatat atattacaac
     2101 tcatggagtt atcttcacaa taaaataaaa agcattattt tcagcgatca gattatctgc
     2161 cagatcaata actccgtcac cattgagatc agtttttata tatccggtat taaaattaaa
     2221 tgcatcatta tcgatcaaac cctgatctgc aagatctaca gcaccatcaa tatttacatc
     2281 tccactatat agagcaaatc tgtccggtgc gatatttacc tgtattaaat tatttccaaa
     2341 tgcttttgca gaactgtttg taagatcaaa acttactgat gtacctgatg aaagaagctg
     2401 tggtgtattg ctccatgtct caatcgaatt tctatgaatg ctgacaagat aaaataaacc
     2461 ggtcagatta gaattgaaat taaaattacc ggtaaaagta acagaatcaa ttacggaaat
     2521 agccgaatca tttttcagat atggagatgt tccgtttctc agaattaact ttatagtatc
     2581 acgaagattt agtttattat ttatctgaga atacaatccc tgcactatta ctttcagatc
     2641 aacagaagca ggcgactcaa tgaatgccga tagattatgt atgtaagcgg cttgagtata
     2701 aattgccggt tcggtaattg tccatgagtt ctgcggccag cttgtattcc agtctctgta
     2761 agatttctga atcggctgag agagtggcgg agtaacaaga gcagtattac aaagagtatt
     2821 actgccgccg catcctgatg gacaacagta atcaagagca taagcaggat tcacacctcc
     2881 ggatacaaaa ccaggcggag gtccatatgc cgaagtaccg actctgtccc acaatgcact
     2941 gccgtcttca aaccagccat gataaatttc atttatagaa ttttccgcac cgctgttccc
     3001 catatttgtc atatagcaga atgcagtcgg atttactccg tgaaaataat ttacgaatcc
     3061 taaagctgca ttcttataat cagtattact cagcgtatct aatttgtatg tgatcatatt
     3121 tttaaacatt actccctgtc tgccctttgt agtgttgctt ccccatgtat aattctgatt
     3181 tgaaatataa gatctgtaag catctgtatt attcagatat gccggtagat tatcggaatt
     3241 gtttgacttc atggaatttt tataaacagt tttgacagaa ttagcaactg aagcagaaac
     3301 tccgatcaat gaagaataat acagcagcat attctgagca ccacattcaa aaggatatgc
     3361 atagctccac tgcatcatat gtatctgtgt ataattgcct tcgacaaaag ttttgtaaac
     3421 agaattaccg gtaagatgat atagaaaaac tgcagcagac attttcctga taagcctttc
     3481 atatgagtcc acttcctgtt ctcctgcacc tataatacct gaattataaa aagtaatgtt
     3541 aggatttgca actccccagt tccaggcatt tacagccgca ttcgttaaag tatttgcaaa
     3601 tgctgtctgc ccgatatttt caaactgaat tgcagccaga gcaaaaatag acgcagcgct
     3661 gaatgtagct gatgtagttg ccggtccgta aattctatgt gcaatatcag cagaaggagg
     3721 gcttgtcgac atggtattat ttgcacctac tattgaaagt actgatccat ttgaattctg
     3781 catcttcagc agccagtcga gttcatattt tatttcatca agtatatcag gaattccgtt
     3841 tccagattca gggatattaa aatcatctcc ccagatttca ggattctctt tatatgcaag
     3901 cagcagatca gtcacagctt caaaagtaaa gttcacatat ttattataat cacctgcatc
     3961 atgccatccg cctgaaagat cttttgaagt aaaaacattt gaattattgt aaagccggca
     4021 gtcagtatcc tgaagagttc cattgtgaca atgcgtatca gtccagcctg tccctgcata
     4081 aggtgcagac tttggtgttc cacagcgctg ataataaaat actctcattg cctgtttcaa
     4141 aacttcatta taaactcccg taccaatctc aaatctatat gaagcagcat tatttacaga
     4201 atcgtaaaca taataagaac cgggtgtggt aacatttgaa aaatcaaacc accagacctt
     4261 gtcaccgctc tgaagatgtg tcgaaccacc gtacattgaa gttattgctg ctgaataaac
     4321 aactgcatca tcgtcccatt ttctcaattg ataaatattt ccgggagtat aaggactgtt
     4381 attgttatag cccgtcaccg gattacttat cacggcaatt tttttatcac cgggtctgta
     4441 accaaactga tctgtcttta tgtgatcgtt tgtatttgat ggaatggctg gtaagttact
     4501 gctgaaaata aatagagaca aaaacaaact gcaacttaag agagaaatga ttttttccat
     4561 aagggttttt taatttagtt tcaggaaaaa tttgaatgat acttaacaat aaatatatgc
     4621 tctatgtagt ttcaaaataa gtaaattaaa attaaaatgt atctactcca gctttttctg
     4681 aaatctgttg gagctgacag caagaataaa gacacccatc aggaacagca ccagcagttc
     4741 tttccagaga tcgtaaaaac caagtccttt taatacaatg cctcttataa ttattataaa
     4801 atattttagc gggataatgt tagtgatata ttgaattgcc tgaggcatgc tgtctatggg
     4861 gaaagtaaat ccggacagaa atatcatcgg cataatcaca agaaaagcag atgtgatcat
     4921 agcctgtcgt tgagtctttg aaattgtcga tacgaacaag ccaagtccga gagtagaaag
     4981 cataaaaata aatgatgaaa aaaagagtag caccagactt ccttttactt ctatcccaaa
     5041 ccagaatctc attaccgaaa gtacaagtat tattgcaata aatccgagca gtgaaaacgg
     5101 cactagtttc ccgattatca tctgataagg tctgatagga gtgacaataa gctgctccag
     5161 tgtaccgact tccctctctt tcacaatcgc cagcgaagtc atattaattg taattataat
     5221 tagaatcaat cctactatac ttggcagcat aaaatttctt gtagtcagat tcggattata
     5281 ccaaacacga atttcaggag tcactctacc cgccagtgat attttcaaac ccgaacgttc
     5341 ctgaatgtcc agagaaactt tttctgaaaa gctactaaga atattagcta cataacctga
     5401 agctataccg gctttcccgc cgtcagatcc gtcaaatatt atctgaatgt cagccgtttt
     5461 tttgctcaga atattctttt caaaattatc cggtatcaca atcccgcaga gaactgttcc
     5521 tttatctatt gcagactgaa gctcttcata atttgtattg aattctttta taataaaaaa
     5581 tcccgaacct tccagatccc ttatcagctc tctgcttttt gaagatctat ccatatcata
     5641 cacagtagta tctacataat tcagatcacg gttagctgcg tagccgagaa agatcaactg
     5701 tatcactggc gcgatcaaca ccattacaaa cattttcgga tctctgaaaa attgtttgaa
     5761 ttctttttta ataaataatt ttattatact catccgactt cctccctctt tattctgatt
     5821 gcggagattg aaataaaaat accggcaaaa attattaagt aaactatttg ttcataaaag
     5881 accgagatgt ccgttccttt aataagtatg tttcttaata ttactataaa atattttgaa
     5941 ggagttatgt tcgacaaaag ctgaattaga taaggcatac tctcgatcgg aaaaataaat
     6001 ccggataaaa ttattgtagg cagctgagat ataagctgtg agatctgaaa tgccacctgc
     6061 tgtgaatccg ctactgtcga aacaaaaact cccatgctaa gagtagcaag taaataacaa
     6121 acagtcccga ggaaaagtac agcatagctt cccttcactt ccacaccgaa taaaatgtaa
     6181 cccataataa ttatactggc agctatcaac agcgaaagta tcaggtaagg aataattttt
     6241 ccgagtataa gctgtagtgg tgtaaccggt gacactctga tctgctcgat agtgctgaat
     6301 tctttttctc tgacaagtga tatagaagta agtatgacag aaataattat cagtatcatt
     6361 gccattaatc cggggataaa aaacaaagtc gagtcgagac tcttattgta ccagaattgt
     6421 gtctcgacat taagaggtac atttgatttt attcctttca gagaaagttc ttcctgtgtt
     6481 aacttattat tgaattgtcc cgtcgcagca gtcagatagt tctgtactat tccacccgta
     6541 cttgcatcca ctccgtcaac tatatattgt atctttactt ccttttcaga ttgcagatct
     6601 ttttcaaaat ttaacgggat tacgagagcg gtataaatct ttccagtatt taaaagttca
     6661 tttgtttcac tgtcttttct tatttgctca gtaacggtga aataagaaga tgcattgaga
     6721 ctacttataa aatctctcga cattgcagaa ttatccttat caaatactcc aatctttaca
     6781 ttatttacat ctgtagtaat tgcatatcca aaaagtacca gcaatgcgac cggcagtaaa
     6841 aaaattatca gcagtgaact tttgtccctg aatatctgtc tgaactcttt caccgttatt
     6901 gataatattc ttctaaacaa atactaaata ttaaatatta aattttaaat attaaatatt
     6961 aaaaaattga acatcgaaca ttgaactttg aacatcgaac atcaaacatc gaacattgaa
     7021 cattgaacat tgaacatcga actttgaaca ttctacattg aacattgaac attaaccact
     7081 attctctttt ctcgagtaag tttaaaaaca catcttcaag tgaaggtatg attacatcta
     7141 ttctgacatt atctctgcct cctgcagtat cagctattac atcatttaat gcttccagct
     7201 ctttatcacc tttggaatat ttctttttta aaatgagatg gatcgaattt ccaaatgctg
     7261 aaacgctttg tgtgtaatct tcttttgcta atttttcaat tactttaaaa gaatccggac
     7321 agtctatttc caggaccgta ctgtcaatgt attcttcttt aagttctgcc ggactgcctg
     7381 ctgctatcag cttacctgcg ttaatgagaa tgatcctgtt gcagaattcc gcttcatcta
     7441 agtaatgagt tgtaacaaaa acagttgttc ctttttcgga aatttcatgt atcagatccc
     7501 agaactttct tcttgaaatt ggatccacgc cgcctgtagg ttcatcaaga aaaacaatct
     7561 tcggctcatg cattactgcc gcaccaagtg ccagtcgttg cttaaagccg actgataaat
     7621 ttgaagtaag agtattttcc ctgtcctcta atcctgaaat tttcaaagcc catgcttttc
     7681 tcttttcaag agcttcaccc tgaagtccat atatcccacc aaaaaaattc agattttcat
     7741 caattgtaag atcaccgtaa agggaaaact tctgtgacat gtaacctata ttttttttca
     7801 catcatccgg ttctttatca atactgaatc cgctgaccaa agcatctccc gaagtcggct
     7861 cgagtattcc gcaaagcatt ttaatcgtag ttgattttcc cgcaccgttt gctccgagga
     7921 atccgaatat ctctccttta tatacatcaa agctgatgtc atttactgaa gtgaagtcac
     7981 caaatttctt agtcagattt tttacttcaa ttgatttatt attttccatc tgatttaatc
     8041 gttatcagtt tccattaatt caataaagac attttcaagt gatggaagct tcatacggat
     8101 atcatcaatt tcaatattat tatttttaag caacaaagta atctcttcta attccatagg
     8161 attaccttta aatacaatat tcaatctgtc accaaacatt tgtatatcag cattctcaat
     8221 atttttcaaa attttatatg cgtctctgac aggattacag actacttcca tgatcgccat
     8281 tttgagcgag gattttattt tagccggtgt accctctgat attactttac cattattcat
     8341 catcacaatg taattaaatc tttcagcttc atccatgtaa ggagtcgaga tcaggattgt
     8401 aatattttct tttaacagac ctgagagaat gacccagaat tctctgcgtg aaacaggatc
     8461 tacacctgtg gttggttcat caagaaatat aatttttggt ctgtaaatta gagtgcaggc
     8521 tagagccagc ttttgcttca ttcctcccga aagttttccc gcctgaaaat gacgaaagtc
     8581 ttttagccgt gtaaagtgaa gcagttcatc tctcctcttt tcaaaatcat ttactccgtg
     8641 aatgtcagct atgaactgaa tattttcatc taccgtcaga tctgtgtaca gactgaattt
     8701 ttgagacagg taaccgatct catttttaat aatacttttc tttttacctt caccaagatc
     8761 aagatctgtg aaatctactt cccccgaatc agctctcagc agaccgcaca ttattcttat
     8821 catagtagtt ttacccgcac cgtcaggacc gacaagtccg aatatcttat tctttttgac
     8881 tttaaaactg atgtcatcta atgctgtcac ctcttcgaat ttttttgtaa cattcttaac
     8941 ttctatagct gattcgttca taataatatc agtttaaaat tatattagca tcagcaggca
     9001 ttcctgactt cagttcatag gatgcattcg gaattcttat cttaatccca aagacaagtt
     9061 tggttctctc ttcttttgtc tgaatattct ttggagtaaa ttcggcttca ggtgagatat
     9121 aaattacttc acctgaatat tttttatcag gatatgtatc atttgtaatg tcagcttctt
     9181 gaccaagttt tattttcccg agtttcgctt cagttacata aataaataga tctacaactt
     9241 tgagatcagc tatgttcaga atggaagaac ctgtattcac aaattcaccc ggctctgcaa
     9301 gttttttggt aataattcct tcaagcggtg aacttacttt acagtcttca atattttttc
     9361 tgatcagatc tgcatttgcc tgagaccttt tcagatttgc tgcggcagat tctacctctt
     9421 ctcttcttat aatagttctt actttcatca gattttcttc agccgaatta agctgatttt
     9481 ctctcaggtc ataaaccagt tcggcatcct ccagattttt tactgttgcg gcattttcat
     9541 ttacaagatt tttcattctc tgcagatcgg tttctgcctg cttaagattt atttcagccg
     9601 cgcttacatt atcttctgca attctgatat cttcagatct cgcgccctgc tgcagaagtt
     9661 taagttgtgc atctgcctgc tctaccgcag caagtgcctg tctaagctgt atatcaagca
     9721 gatcatgatc tatcagaaac aatacatcac ctgctttcac catgtctcct tctttcacat
     9781 aaatttcctt tatctgtccg ggtgtcttcg agcttattac aatatttgta gcttcgatat
     9841 ttcccgatcc tgaaatggca tttgtatcca cttcttcttc acatccggaa aatattgaaa
     9901 ctgatattat tatcaggaaa attgagctga ttaagaactt tgaagtaatt tttttcatgt
     9961 tctaaaaaat aatttaacag tgattttttt atattctaat tgaattaata ataaattccg
    10021 gaatttcctt ttttctttta ttcaaaaatt tttcgaatcc cgcttcattc attccgaata
    10081 cattagatat caatggtctt gcaagaaaag gaaatatgca aagtgagatc aagtttataa
    10141 atagctgcac aggatcgacc ggtaatattt cttctgattt aattgcttcc ctgatctgtc
    10201 ttataaagat ctcgggcttg atcttaattc tttcagaaaa tgttttcaaa aatttatcgg
    10261 gatcattatt catttccctt atcacaaaaa caggtataat tggattttgg gacaataaag
    10321 taatgtattt gtcagtaaag tattctattt tttcaaataa aggcttttcc atattcagaa
    10381 tttcaatcac ctgaggaaaa attttttgag tagcttcttt aaagactgca tcaaacaacg
    10441 cttccttatt tctgaaataa taatggagca ttgccttatt gatccttgct tcatcagcga
    10501 tctcctgcat tcttgccccg tctattccct tattataaaa tatttttttt gcagattcaa
    10561 gaatcctatc ttctgttttt ttgtctttta tttttttcaa catgctaaat tgctaactaa
    10621 aaagattaac cgtttggtta agaattaaaa cttaactaac gcagaaattt atttcaacat
    10681 aaaagaaaac ctctgtacaa tttaaaacag aggtttaatt ttatttttta aatttgaaaa
    10741 agattttaaa ttttcacaga agtagatttt tatttgaaat ttgtaatttg taatttcttt
    10801 ccgcaagaac aactttcgct cccccttttt taaatgggaa ttacttatga attattctga
    10861 aacgtgatac tgaaaattta gattttgtgt caaataattt cactgaaaaa tttattattt
    10921 tttttcgata aatttaaatt gtaactaatt aaaaagtatc tcataattca tatgtcctaa
    10981 gttgatattc gtatctcgta attcaaattt cgtaactcgt aatttctaac tcataattcg
    11041 aaattcgtaa tacttaatta gtaatactta attagtaatt tttaataagt aattgattct
    11101 tcatctgtag tcataataaa aagtgatcct cggtactaca tctgtcttca atcccgctgc
    11161 agatgaattt ttctgtgaat aagcattcag ccagatattc ggcatcaggt gaacattctt
    11221 atgcggcata aaatcaattc ctgccgttac aaaatattga ttaaatcctc tttgggcagt
    11281 tgtagtattt tcattataat aatcaattct cgaaaaaaat ctgaatttat tttcactaat
    11341 atttcctgta gcaaacaaac ttaatcccat tggatctgta ttatttgtga ccgagttgaa
    11401 tattttattt ctctggaaaa atccttccag tcctattgaa aatgattcca ctgaataagc
    11461 aagaaaagaa ctaaaagtat atctgtcatt attatcgttt acattttcaa agtcagcata
    11521 aatatctgct gttatctttt tatctgcaaa attaccggac agcattccgt aatacttttt
    11581 atatttgttt acctcttttc tgacacctga tccggttgct atcataaaat agtatccgta
    11641 attttttttc tcatcaaaat ttccgttaag catgactcca aggtctcttg agcctgcgat
    11701 tcctctttga tccataatag tcttttcaac agacctgtaa ccccatattt tttcttcaat
    11761 aaatgaatat gaaggagtgg gctgtaatcc gatagtaagt gtagatccgg taaatatttc
    11821 tttccatgca agaaatgcat cctttatcag cactgcgttt tttaaattag gaagtacatc
    11881 attcccgtca taagcaagaa caaatcttgt aaagaaatac ctgctgatgt tatgatcata
    11941 accgagataa attcttctga agtcaaatga attataatct ttaggataat tagtataatc
    12001 taaaagagaa atggtagagt cgcctccggc tttataaaag aaatcaccga atgcataacc
    12061 ccagactttt ccggtaccgt cttcctcatt ttcatccgga tctttgttac ttgtattttc
    12121 cttagtacta ttgggatctt tcagatcttt tagctttttt agatctttca gatactttga
    12181 atttttagtt atttcagttc catttttttc gtcaaatgtc tgagctgata aaattcccgt
    12241 gatcaaaact atgcatagaa tagtaagtaa aattttcatt taataaattt aagttaataa
    12301 ataaaaggta aattattaat tttatttaaa atattgttta gcataatatt atttaatgat
    12361 ttatatcata ctaaatttta attttatagc gatgtaaaat attttttaat gcattccaaa
    12421 aatacagttt tctgtttaaa gtatgaatta ataaattcaa agctaaaaat attttattta
    12481 atcgaaaatg aattccaaag tattaattat ttaatttatt tgtgtatcag ttaaaaaatt
    12541 tattcaccag ctaaattcaa ctattacaat aacattatta aatttttcaa taatgatttt
    12601 acaggcattt atgtaaattg attgttaaaa attattcaat tgtaaaaaag cttttttttt
    12661 aagaattttc ataaaattta atcagctatg aaaaatattc tattccctac agacttttcc
    12721 caaaatgcgg ataatgctct gaatttagca attgatatag caaaaaaaac taatggtaca
    12781 cttattctat ttcatgcata cagtgtgcag ttagttgatc ccaatatgcc tgccgagatc
    12841 tatctttcag cttatcagga agaagaaaaa tcagcaaagg aaaatctgga ggaattgaga
    12901 aaaaaaataa tggacttcaa taaagatgat accggtaaaa atttatttga agtcgaagca
    12961 atcgtttcac agggacttgt agtagatgaa gctttatcaa ttatagatga attcaacatt
    13021 gatatcgtta tcatgggaac acacggcgcc agtggtctga ctgaattaat actcggcagt
    13081 aatactgcca gtctaattga gaattctccg gttcctgttc ttgctattcc tcaaaatgta
    13141 aaaaataaat ccattgataa cattgtctat gcttatgatg atattaaagc aaacatgcct
    13201 tcatttcagc gtctcctgaa tttttctgaa atttttaatt ccgaaattac tttactgcat
    13261 attatagaaa caggaaaaga tacagaagaa aaaaataaaa aagcatttga ggaaattaag
    13321 aataatattg gttctgataa gattaaactt gcacttgtaa ctgaagagaa tgtactggaa
    13381 ggtataaacg aatacataaa ctcaaatgat gtggatgtac ttgcaatggc aatcagaaag
    13441 agaagtttac ttgataaaat tttcagcaga agtcttacaa aaaaaatggc gtatcataca
    13501 aagatcccgc ttctcgctct tcataaagtt taaatcaatt ttactttcaa cttatagagt
    13561 acccggattt ttataattaa taatcaaaat aaataaattc ctttggctga aaaattattt
    13621 cttatcgatg caatggcagt gatatacaga gcttattttg ctatgatatc aaatccgctt
    13681 ataaactcga aaggaagaaa tacctctgca gtttttggct ttgtgaattc cctgttaaag
    13741 attctcgaag agcaaagtcc tgatcacatc gcagtttgtt ttgatactgc tgcacctaca
    13801 ttccgtcata aagaatttcc ggcatataaa gcacagcgcg atgtgattcc ttctgatatg
    13861 ccgtggcaga tcgaaaaagt aaaagaagtt ataaaagcat ttaacattcc gatgatcgag
    13921 cttttaggtt atgaagctga tgacataatt ggtacacttg taaagcaggc ggaaaaagaa
    13981 gatgttataa gttatatggt cactcctgac aaagattata tgcagctcgt ttcagataaa
    14041 gtatttatgt tcaaacctgc ccgcacacaa tacggcactg ataatgagat catagattct
    14101 gaaggtgtta taaaaaaatt cggagtgcct cctgataaag taatagaagt acttggtctt
    14161 atgggagatg cttctgataa tattccggga ataaaaggta taggtgaaaa aactgctgcg
    14221 aagcttatac aagaatttgg ctcgatcgaa aatatgtaca ataacattga caagatcact
    14281 aaaccaaaac tcaaagaaaa cattgagtct caaaaaaatg aagcgcttct ttcttacaga
    14341 ttagtcacga taaaaactga cgtaccgctt gatattaatt ttcataattt aaataagaag
    14401 ccagaagatg cacctttact gactgcactt tttgaagatt tagaatttaa aacacttaca
    14461 agaaaatttt caggagatca atctgataca gatgctgaga ttaaaggttc agcaaaaaaa
    14521 gctgcctctg caaaaagtat tcccgatttt gcgaaagttg atattaccgg tccggataaa
    14581 gtcttaaaaa caattaagga cgtaaagcat gattatataa ttattaagac tgaaaaggaa
    14641 tttgaagaac ttataaaact tctttcaaag gaagacatta tttgttttga tactgagaca
    14701 gatgctctcg atgcaatgaa tactaacctt gtaggaatgt catttgcatt tgaggaattc
    14761 aaggcatact atgttcctgt aacaggtaag atgtttgata agaattttaa aaaaggaagt
    14821 aaagctgaaa aggaaatatc tttatttgat acttcgaata atgttgataa taaaatatct
    14881 aaaaacgaag cagataaagg aattgatata agcacagcta taaaaatggt aaagccattg
    14941 ctcgaaaata aaaaaataaa atacgtaggt cagaatctga aatacgatta tctcgtgatg
    15001 agaaattacg gcatcgaatt atcggatatg ttttttgata ctctgattgg ttcatatatt
    15061 ctcagacctg aaggcgcgca taatatggat gcactttcag aaaaattcct tggctataaa
    15121 ccaatttcca taaaggaact gatcggcaaa ggcaaggatc agatctcaat ggataaagtc
    15181 gatattgata ttgccggtga atatgcagca gaagatgcag atgtgacgct tcagctttat
    15241 tacattctaa agtatgagct tggtaaatta gatatgagga agctttgtga tgttgttgaa
    15301 tttcctctga tcaaagtact ttcggaaatg gaattcgaag gatttaaagt cgatgaaaga
    15361 attttaaata ccattgataa acagacacaa aaatttataa tagaatacga agcaaaaatt
    15421 tatgcagccg caggagaaga gtttaatatt aattctacac agcagcttgc ggaaatttta
    15481 ttcaacaaac ttaatcttac tccgactaaa aagaccaaga caggtttttc taccgatgta
    15541 aaagttttag aggatttaaa atatcagcat gagatagctt ctttgttgat tgactacaga
    15601 atgctgacaa agctgaagtc aacttatctc gacggactaa agaaagcagt taatccaagg
    15661 acagggagag ttcatactgt tttcaatcag gtagcagcag caacaggcag gctatcaagt
    15721 gtcgatccga atctacagaa cattccgatc agaactgaag ccggaagaag tctgcggaag
    15781 gcattcgtgc cgagggataa ttcttatgtg ataatgtctg ccgattattc acagatcgaa
    15841 ctccgcatca tggcacatta tgcaaatgat gaaaatatgc tgaatgcatt taaaagacat
    15901 catgacattc atactgaaac ttctatgcga atctttggac tgaaatcaaa aaccgaagtg
    15961 acagcgggta tgagaaggaa agctaaagaa gtaaactttg ggattattta tggcatcgga
    16021 gcattcggtc tggccagcag attagagata aaaaattctg aagcaaaaga gatcattgag
    16081 agatatttcc gtgagtatcc aaatgtcaga acatatatgg aagagacgaa aaaatttgcg
    16141 catgaaaacg gttatgtcga gacgcttctt ggcagaagga gatatcttcc tcagatcaat
    16201 aatcaaaatg cagcagcaag agcagaagac gaaagagctg caatcaacat gccgatccag
    16261 ggaactgcag cagacatgat aaaaatagct atgattaata tttatgttga atttcaaaag
    16321 aacaaacttg aatccaaaat gctccttcag gttcatgacg aacttgtatt tgaagtaaag
    16381 aaaactgaac tcgaaaaagt aaagaagata gtaacagata aaatgaaaaa tgctattgct
    16441 ctgaatgtga acattgaagt ggaagtcggt gaaggtgata gttggtatga agcgcactga
    16501 tcaattttaa attttaaata ataaatattt aatagtaatt cgtaacccgt aacttataaa
    16561 attcttaatt cctaattttt aatttttaat ttttaatttt tttaaatgat agtagtaacc
    16621 ggcggagcgg gatttatagg cagtgcatta gtgtggcgtc tgaatcagct tggagaagaa
    16681 gatataatca tagtggatga ttttacaaaa gatgaaaaag aatctggtga caaatcattt
    16741 tcagcaaaag atgataagtg gaaaaatctc gttcatctga aatatgctga tgtatttgac
    16801 aaagaagatt tcggacatat gctttcatct gactttatga aaaattttga agttgaaacg
    16861 gtatttcatc ttggcgcaaa cagttcgaca actgaaaggg actttggttc tctgatgaca
    16921 aataattatg aatacacaaa atatctgtgt gaaaaagcga ttgaaaacaa tgtgagattc
    16981 atttatgctt catctgcagc aacttacggt gacggttcga aaggttatga agatgatgaa
    17041 agcaaactca acacacttgt tcctctgaac ggctatggct actcaaaaca catgttcgat
    17101 atctgggcaa aaaaactaaa gctgctagat aaaattgtcg gattaaaata ttttaatgta
    17161 tatggcccga atgaatatca taaagaagac atgcgaagcg ttgtgaataa atgttatcat
    17221 cagataaaag aaacgggtaa ggcaagatta ttcaaatcag caaaccctga attcgcggac
    17281 ggtggtcaga gtcgtgactt tctttatgtg aaagatgcaa tagagatgac attatttttc
    17341 agagaaaata aaattgtcaa cggtatttac aatatcggta cgggtgagtc caattctttt
    17401 aatgaatttg taaaaccggt tttcaaagcc ctggacataa aggagaatat agaatatttt
    17461 gatatgcctg agattttaca ggaaaaatat caatactata caaaagccga tatcactaaa
    17521 ttaagaaatg caggctacaa gaaagagatt tataaaatag atgatgccgt gacggattat
    17581 gtagcgggat accttaatac tgaagatgct tatcttaatt aataattaat atttaataat
    17641 taataattaa aaattgagaa ttaatttaaa acaactaaaa taaatcactt aagaaaaatt
    17701 agaaaatttt aaacaataaa cttataaaaa tgaaatccat tttattgatt ctactctttg
    17761 ttactttgac aggaaaatct ttttcacagg acttgccgca ttatatgaca gataaagaga
    17821 aagaactttc aaaaaattat cagtacaatt ttttcccgac tgacaataca aatccaccgc
    17881 cgactcctgt caggacaatg gctgaatggg aggaagtgca gggagttata gttgcatgga
    17941 cttcttacac tactataatc agacagattg tcgattatgt acaggatgaa tgtcaggtat
    18001 ttatagtctg cagtgattcc aattctgtaa agacatttct taccagtggc ggagtacctc
    18061 ttgtgaatct cagatttatt gttgctccgt tcaattcagt ctggtgcagg gattacggtc
    18121 cgtgggctgc atactcggga atttccgaca gcctgaaaat tatagactgg atctacaaca
    18181 gaccgagagc tcaggacgac cttgttccga ctgcatttgc aaattataca aatcttccga
    18241 tctatgaagc aactgttgct ccaaataatc tcattgcaac cggaggaaat ctaatggttg
    18301 acggaaacgg cacagctttt tcatcaaaac ttattataga tgaaaatcct accaagacag
    18361 aatctcaaat cgacgggatt atgaacagct acttaggagt ttcgagatat ataaaaatga
    18421 atacacttcc atatgatgag atacatcata ttgacatgca tataaaactt ttggatgaag
    18481 aaactttact cgtaggtcag tatcctccgg gagtagctga cggtcctcag attgaagcaa
    18541 acttacagta tgtattaaat aattttcaga catgttatgg gagaccttac aaagttgtaa
    18601 ggattccaat gcctccgagc tctggcggtc agtatccgcc aagttcaaat tattttactt
    18661 atacaaattc tgtgtttgta aacaaaacta ttattgttcc gatctatggc cttgctcagg
    18721 attctactgc tctcaggatc tacagggaaa atcttccggg atacagagtt gtcgggctca
    18781 atgcaagtca gatgatctct gctctcggtg caatacactg tatcacaaaa gaaattggtg
    18841 tcgccgaacc gatattcttc tcacatccga aattacataa tacactgaat accacttcac
    18901 catatgaagt gaaatcttat attaaaacca agtcaggcgt tgcctctgca aaagtcttct
    18961 ggagaactga tacgacacag tcttacaatg aaattaccat gacaggaagt ttggatacat
    19021 tcagagcaaa cattcctcag caaaatcttg gtacaaatgt ttattattat atttcagcta
    19081 catcgaattc aaatcgaact gtcacaaaac ctattactgc accgcaggga tatttaaaat
    19141 ttgtgataga caatccgact tctgtaatct caaataattc tcaggcagag aattattctt
    19201 tgtcacaaaa ttatcctaat ccctttaatc caaaaacagt aattaattac aaattgcaag
    19261 ttacgggaaa tgcgaagata atggtttatg atgttcttgg gaatgaagtt gcaactttgg
    19321 taaatgaaaa acaaaatgca ggcagctatt cagttgagtt tgatggaagt aatttttcca
    19381 gcggaattta tttttacaga atctcttccg ggaattttaa tgagacaaaa agaatgatgc
    19441 ttataaaata ataaatttta aatagtaaat tttaaatagt aaattttaca tttccgcaga
    19501 gtcagaaaag cagttgactc tgcggagaca ttagagacat tatcatttat aatcgtgtgg
    19561 gaatttataa atttagattt gcagtcctca gagtccataa gcatttgact cagaggagac
    19621 attaatgtaa aattttattc ggtatgagtt agctcaccaa agcaaaatct attttcttac
    19681 tttctttatt tacccggatg acttttattc tgaccttctg tccggctttg agagtcttct
    19741 tttttctcct tccaaaggcc atatgtcttt tttcatcata tacataatag tcatcttcca
    19801 tatctctgaa tctgatcatg ccttccacca gaatatccat gatctcaaca aatatcccgt
    19861 actgcatcat gcctgatatt atgccgtcgt attctgagcc aagatgtttt gaaatataat
    19921 ctgtctgcat cagttttata gattcacgtt cagctttttc tgcattctgc tccataatag
    19981 aactctgctt gcagatttca ggaagtacct tttcataatg taaaattctt tcacgtaact
    20041 tcttgccgcc cttgagataa tcaaatagac atcggtgaac tgcaagatca ggatatcttc
    20101 tgataggcga tgtgaaatgt gaataatctt taaacccaag tccgtaatgt ccgatatttt
    20161 ttgtctgata aattgccttt gccatcgatc tgataaaaag atcattgata ataaattctt
    20221 cgtgcttgcc ttctatatca ttcaaaagct gtctgagaga ttctttgtct tcgacgttta
    20281 ttttataacc aaactgtttt acaaattccg aaagctcttt catcttgtca ggatttggag
    20341 tgtcatgaac tctgtaaata aaagggtaat ttattttttg ttctttggaa atattaccga
    20401 caaattctgt agcacattta tttgcaagca gcataaactc ctcgatcaga cgcattgagt
    20461 ttagtctttc ctttactact atctcagcga cactgccgct tttatcaaat ttaaatttca
    20521 cctcatttga atcaaaatca agactctctt ctctcagtct ttccagagta attgatttac
    20581 taagcttgtc cattactttc agctctttat aaaaatcacc tttacctgtc tcaattattt
    20641 tctgtgcctc ttcatacgta aatcttcttt tactgttgat aactgttttt cttatttcaa
    20701 aactttttac tttcagagat ttgccaagtg tgatcagtat tgaaaaagta agacggtctt
    20761 cattaggttt cagtgaacaa agttcatttg aaattttctc aggtaacata ggtattacgt
    20821 tcttcaccaa atatgcactg gtagctctac gcaaagcttc cttatcaagc tcggtatctt
    20881 cctgtacaaa atgcgataca tcagcaatat gaacaccaag tatatatcca ccgcctttga
    20941 tcttctctac tgagacagca tcatcaaaat cttttgcatc ttcagggtcg atagtaaaaa
    21001 ttactttatc tctgagatca agtctgtctt tgggatcata ttctattttc agaacttctg
    21061 cttccttcat tacttcattc ggaaattctt ttactaatcc gaattttctc atgattgaaa
    21121 cctcttccgc tcctcttact ccggacttgc caagtacttc aagaactttc gcacgcagat
    21181 cagtaaattc atctccatga tcccccggat ttaaaatttc tgcatacact ttgtcattat
    21241 gaactgcctc attaaaatcc ctgaaagaaa caatgatctc cttttttatc tctctcgaat
    21301 ccggaaatat cagaccgtag cttttgtgat cttcaaattt tcctgtgaca aattttttaa
    21361 tatgagtgac aatttcaata atctctgcat catttccttc atcagattta agagagttga
    21421 attttacaat atcaccgatc ttatgggcat acgtcctgga gctttttata ttataaattt
    21481 taatctcatc actttcagat ttaactttaa taactggtct cccgtctttt ccaaaatcaa
    21541 tttcaccagt gatatcttct ttatttatgt ctgcagtttt ttcgaattta taaccgctga
    21601 cctttaatct gcctttgtta ctgctgtcgg tcttaaacct aagattcttg ctgccgcttt
    21661 ttttatcaga agatttttta acaattggct ctgcaagttt atttccagta ttcaaactgt
    21721 aatattttct atcccgctgt atacttcctt catttacaag tttatgcaga tgttttttaa
    21781 gagtttctct ctgagatggt ttaagattca gtttttttaa aattgaattt accttaaaag
    21841 aagtgtcttt gtttttattt aaaagctcga gtattttttc ctgagtatta atatttttaa
    21901 aatttaattc tataataaat caatgcacct gctctcgtac ctgatacaac tgatgcatct
    21961 gtggatgctg aagaaaacgg atcgtagact ctttctactc ttaagacaag atcatctgat
    22021 aagcttttca aatttaacat tttactgacc gggtaatcta ttattatatc tgtatttgct
    22081 attcctttaa atatctgacc tccgaaccgg ataaccgctc cgcctatagc tgctgtaaat
    22141 cttatatcag tattttctgc aatagaacct gtcttggaat ctatataatt tacatcggta
    22201 tttatgagaa atggaagtac agtctcaagt gaatttgtga cataatttga caacacagaa
    22261 gcaccgagtg aagcaccgaa gctggaactt tcgctgaatg ataactgatc tttgaattta
    22321 ccaaatagta aaaatgaaat ggcgtcactt gttgcgttac tgcctgactc tctcgtcccg
    22381 tttctttcta agttcattgt caaaactgga ttctttgctt ctcctgttac attcatatct
    22441 atcactactt catttatata ctgttcacct gatccgccga tatcaacaga tgtataacct
    22501 ttgtatttag catcaatgtc gagcatcgga tttgttaaag gtccgttaaa aataacttta
    22561 cctgtcgcat caaattttct gaagaattta taatagcaat tattacctaa agtgacttcc
    22621 cctctgccgg acatttgata attttccttg ttgtcaacct gaagtcttgt tttaatttca
    22681 ccaaaaaact cctgttgaga tttttcatta actaaaaact ttaagaaaac attgcctgaa
    22741 gttgtaacta acagatcata aaaaaataaa cctgatttct ctctctgctg atattttatg
    22801 tttttattat tatctgtata aaggactttt tgaaatggat tcagattcag tccctttatt
    22861 ataactacac tatccgatga attttttaaa actcttttta caaattcgtt attataattt
    22921 aacgaatcta ttataactcc atattcaaaa tcatcagaat aaatattata agcttcctgt
    22981 acaaaaggat tgaatacaat atttcctctg ataagagtta aatttcctgt aagatctatc
    23041 ctatcggaat ttccttttat ctttaaggac ggacttccgc tgccaatcca caagtcacct
    23101 gatattccaa gttcagtagg tccgttatct ttgtcaaatg cttttacatc tccagacata
    23161 ttcaaatcaa tatcattcat tttcagatcg gtaagatcga cataacctgt aattgcaata
    23221 aattttgttg gatcttccgg aacaaatatt tttgatttat taataagaat cttattattt
    23281 cctgttgata ttcccgcatc aaaatcatat ctcatcttgg taagtgttac ataaaattta
    23341 cctttattta aattcatatc tcctgttaac tgaggatctg atgttattcc taacagtttt
    23401 atctccccat caagaattcc ttccagatct ttggtgtatg gaagcagctg ctgaaaaatc
    23461 tttagctgaa agttgtctgc ttttgcggaa atatttacgt ctgattctga gcgtttttta
    23521 ttatagacaa ctgtatcatt atattcatcc ttagccttgt taagaaaagg aaatgttccg
    23581 gtgagtttga aattacctgc gttatttata ttgtaaaaag caacatcact ttttattact
    23641 tcatctttat aatctacaaa agcatcaagt ctgcctattt tggtttggcc aatcgctaat
    23701 acatcggagt ttgtttcaag actaagatca atattgtccg gcataccctt atatgtaagt
    23761 aatgtccttc ttaaatttcc ttttataacg tccactgtat caaagtcttc acccggttta
    23821 attaatttca gtatagtata aacttttata ttatcagcac tgacttttat attactactg
    23881 tcattcaaag aataaaaacc gtttactgta accttttgat ttaaactgtt gacaacgaat
    23941 tttctgaaat caaagatctg agaactgtca actttagtat attttatgat aaggtcagaa
    24001 ttgtttgcca gattcatatc atgatatttg acagacaatg aatcaaacac aaacaaagca
    24061 gaatctttta atgaaaggtt tgtatataat tttatagtcg tgtctctatt tgctgttaga
    24121 aaaacctgat tgttattatt gaaaaaattt atttttgtga atacagaatc aaatttattt
    24181 tttgctgcaa tgactttatt ggaatgtaaa gtaatgtcag tataaaattt attcatgtca
    24241 tttgtattta aatcatttct cataaacaaa ttcagatttg catcttttag aataaacaaa
    24301 gaatcgccgt atgcaaaatt gctgatcttt ccgttagtat taaaagtaaa cagaccgcag
    24361 ctatctgaga tacttccttt caaatctcca ttgaaaataa ttgtatcacc tattaatgaa
    24421 tatataggtt cgaggtctct gacattgaca gtataattca tataataatt attgcaatca
    24481 attacatatc cgctttcagt gatcaccggc tgaattcccg atgtgtccgg aaaaaattta
    24541 tcagttatac tattcgaaat cttttccgaa ttttctatta caagatcaag aagttttgtg
    24601 aactgaaaat atccgtcagc ttccacatcg gcaaagtcag tctttaatga gatcttacga
    24661 atgttaccat tttgatcaat gtcagctaat ataggtgtgg aaggtaagtt aaatccaccg
    24721 aattcagacg gcttaagatc gatcttgaaa ctgcctgcta tatcatccgg ggaaattcct
    24781 ttgccgttta catcaaaatc aaatgtcaga ttacttttca tttcaggatc ttttgtaatt
    24841 acctgaagat ccagatttga aactgtacct gtcagatcat aagatagatt ttttggattt
    24901 gaaatattta gtttaccttt tgtctttccg gtaacaaggt ttgattgaat gctgacatca
    24961 agattcagat tgcctctgtt aaataatatt ttcccttcag atgctgcaat attcaatcca
    25021 tagaattttg tcgagaccaa acgatatttc agactgcctg aatttttttt ataatcaaaa
    25081 ccttttgctt ctataataaa ctcaccgctg atacttcctt cgagatcttt ttccttaaag
    25141 atctttccga gatttacggc tgaagttttg atgtctcctt tatatacaat ttcatttccg
    25201 cgcagatcca ttttcatgtt accataagtt ttacctgctg aggaaataat atcaaaatca
    25261 gtttcaaaat tatccggctt tccagtatat ctcaattctg gaattgtcac aacacccaga
    25321 tgtgaataat ccggaatcgg cagaccggga accattatcc ttgtatctct tggatctata
    25381 gaaatatctt ttccggaaac atttatataa agtgactccg gtatatgcag gttctttaaa
    25441 tttcctgtaa aatgaaattc agatttttca gtagtaatat caaatttctt aagcttaaga
    25501 tctccgtatg catcttcagc tattaagtga aaacttatag tactgtcaat aaaatccagt
    25561 tgaggtaaaa agaaactcac atcatcagca tttataggat ttgcctgaat attaattttg
    25621 gcgtcctttt tttcaaactc ttcatattct attccctcca atgcatttag atcatccaat
    25681 cggatcttgt ctataagaat atttgatctg tcggtaatga gatttagttt agaaatatct
    25741 gtgatagtat cttttacatt tatatttgct ttcagggaaa gttgattcag attaaaatca
    25801 gaattagtct taaaattcag tttactgata ttcacatctt tttcatcagg gaaatatttt
    25861 gcggagagat taagattcaa ttctgataga tcaaattctg aaacattaaa agtatcgatc
    25921 tgcttcattt taatttctct gataggttta tctgaatttt tattttctaa tattctgata
    25981 aatccgtttt caatagtaaa attttcggca gtaattcccc agtcaaattc tgatgtagtt
    26041 gtgtcctctt ccttatcttc agattcaaga aaatattcga agttccattt taaactgtca
    26101 ttattatccc ggatctttgt cacatttatc tgcggttctc tcaatattaa ttcacgtgca
    26161 aatatttctt tggaaagaag ttttatgata ttatattttg cgctgagtga attgaatttt
    26221 agaagagtat ctcctttgat ctttatgctg ccattattta gagtaaaacc ttttaaaata
    26281 tttccgttaa gagattctgc atgtatcaca gcatcttttt ctgctaatga ttcatttagt
    26341 ttatctaaag caatatcaag tgcgagattg gaaaacatgt cagtctgaat aaaaataaat
    26401 attccaatta tgagaacaac aaagaaaatg attacaaatt taaagacact gaatatcttt
    26461 ctgatcacac tctttttctt aggagtctgt tcagcaatat tctgaatttc agaattctct
    26521 ttattgtcag agctgtcagt aatatcggga ttattttctt ctttggacat attgttcaat
    26581 ctgaataaac agatctaaac tagttagtgt tttctgatct tttcaattat accggttgag
    26641 gaataattat ttataaaatt taagctgagt actttaccgc cggctgcagt tacaatgtca
    26701 gaacctacaa tatcaatttt cctccagtct ccgcctttta ccaggtaatc cggtacgatg
    26761 agttttataa gttcataagg agtatcttca ttaaaaataa ttacaaagtc aacggatttc
    26821 aaattatcta aaataaatgc tctgtcattt tcattattta ccggtctgtc atcacctttg
    26881 agttttttaa ccgacttgtc agaatttaat ccgacaatta aataatctcc cataagtttc
    26941 gcctgattca gataagatac atgtccttta tgaattatat caaagcaccc gtttgtgaaa
    27001 acaagtttct tattttcact tttgattctg cttcgtacat cggatatatt atttatgttt
    27061 aatatcattt tccactttta ataatatttt caattagttc ttttttattt accggtacaa
    27121 tgcctacttc ctgtactact atgcctgctg cgaaatttga gattactgca gaatcacgca
    27181 gactagctcc gcctgaaaga caaacagcga tagttgaaat tactgtatca cctgcgcctg
    27241 agacgtctgc tacttttctt gctttcgtac tgatcttttc tttttttaat ttgccacgga
    27301 tattttcata gatcatcata ccttgttcac caagtgtgaa aataacattt tgacaactga
    27361 tctttttaat tagtttagct gcaatgtcat ccagatcatt taggtctctc agtttaattc
    27421 caaatgaatc ttcaagctcc tttctgtttg gcttaaataa aaatgcattc ttgaattcaa
    27481 aaaaattatc aaactttgga tcgacaagta ttttaaaatt catctttaaa gaaagagcag
    27541 aaacttttct gatcagcttt tctgtcagta ctcctttgtt ataatcctgt aatatcacaa
    27601 tagaatcttt ttctgcatgt tctgaaagaa gagacagtat tttagtttga gaattatctg
    27661 aaatatcatc tttagactcg ctgtcgatcc tcagcaaatg atgtgaatca gaaattactc
    27721 ttgatttgct cgtagtaggt ctgtcttttt caggaataat accctccgta tcaatgccaa
    27781 gatcctgcat tgagtttttc agaagatcgc tttcggagtc ttcaccggta ataccaatca
    27841 gaaaaggttc agagccgagt gtctttatat tgtatgcaac atttgccgct ccgcccagac
    27901 gaaattcact tttccgtata tcaaagacct gtacgggagc ttcaggtgag attcgggtta
    27961 catctccaaa caaatatctg tcgagcatta aatccccgat aatgatgatc tttttatcac
    28021 gggaattttc aaaaatgcct gtaagtagct ttttattcat tgccataaaa atagacttat
    28081 tataaaggtt attcaatgcg aaattatttc ggttctgttc atttaataat taagaattgc
    28141 tcagtatcat tttgttattt tatagttgaa catttaaaat taaataaagt acaacttgca
    28201 ttattcatcc aaaatgttga atttcaaaaa ccaaatttat ctaattatat gattgtttca
    28261 acttttattt acctgttctt cataatatta tcatcaagtg atcatcagaa ttctactgaa
    28321 ataaaagatt tgtataatac cggtgagatc tcaaaaggag ttgtattaat tgacggtgaa
    28381 acagatccgc actataagct aattaaaagt tctgataatg gatatcccgg tcctgactgt
    28441 aaagtttctt tgtcaaatgg atttccgatg gacagatggg taccaaattc aaacagatca
    28501 aaatggattg cgccgagaac tgatgctttt aagtttaatg aaccgggaat atatgtttac
    28561 aggacggaat ttgatctgtc agaatttgaa tctgagaata ttgtaatttc aggagtatgg
    28621 tcaactgata ataatggagt ggatattctg atcaatggaa acagcactgg gtttgcaact
    28681 ccgatagaag cgtattacgg aatgtttcct tttgaaataa aaaatggttt tgtaaaagga
    28741 ataaatactc tggatttttt agtctataac ataagtgcac cgacagggct tagagtcgaa
    28801 cttcagggaa gaggagtgaa aaaaattatt acatcccgtt aaaattctgt atagtgtttg
    28861 cctcaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg aattctaaat
    28981 agtaccagag gcaggcagat taaataaaac ttccaaagtt attcagactt tggaagtttt
    29041 ttatttaaaa tttattctat aaaatttaat attacttgat cagcatcatt ttttttgtct
    29101 gaataaaatc acctgttcgt atagtataga aataaatccc gctcgcaaaa cggctgccgt
    29161 caaattcaac agaatatgta ccggcattct gcttttcatt tacaagaacc atcacttcat
    29221 taccaagcac atcaaatatt ttaagagaaa ctacatcaga ctttggtaat tgataattaa
    29281 taattgtttt tggattaaat ggatttggaa aatttccaac agtgaattct gtacttttta
    29341 tttttgaatt atctgctttc aatttatttt ctgaaatgct gttttctata ttgaattgtt
    29401 tacctttttc atatctgccg tttgtaataa aaatataact tcgcagatat ccgtatttta
    29461 aaggagaatg actgtttttg aatttaagta taagttggtt tgaagaatca cactgaatag
    29521 taatattgtc cttatctttt acaaagttaa gaacatttcc tgtaaaaaga tcttcagctt
    29581 cgacaagttc aagctcttca gtaaaaaatc cggaataatt tattccagta tatgccagat
    29641 atgacatctg aaaattatcc ttccattcaa tagtaagtgc atcaatacct ttatttgcaa
    29701 atacaggcag tattatttca gagtttacga ttctttttga aaagatcaga tcagatttct
    29761 cattgtttcc tttactgtcc tgaatattga tattgccggc gatgtctttc acaacgggaa
    29821 taaccggtgt cagtcctgcg gaacccggat taaatatcaa agcaatactg tcggattcag
    29881 aaggaagaga tgatgcctct ggaaatttag ccgaaagaat ttcattctct atgcctttaa
    29941 tgaatttcgg attttgaatt ccatatttta aataattagt gatatttttt cctgaaattt
    30001 cagcatgact tggtgagata acatatttag gagtgtaaat taccggatca ttattttctg
    30061 tgattcccat ttcagtacct acaggatgat ctgctatcag cagtctgaac tgatcaaaat
    30121 aattcacatc tatatttgtc tcttttactg ccagcattat tgtactatcc tgaggatata
    30181 cagaaggtgt agttttaagt ttgtatttgt cttcaatata tttacctgca tttattaaat
    30241 gctctgatct gtgtaaaagg ttattatcat aaacaaattt ttcaccgttg tttaccaata
    30301 caaatggaca accggaataa ggcggatttg gtctcaggaa ctgatttgga tttatccatt
    30361 gtaaatttcc tgaaccgtaa aattttgtat cagagccaaa ttgatttgat gcagtgacct
    30421 ttattgtaaa atgaagcggt ggctctgcgg aaaaacttcc tgcagaactg ctgcacactg
    30481 aatcatatct caggcacgtc cagtaattct gaaaatttat atttgtcata ctgaaaatat
    30541 tctgattctg tgataaagaa attccctgaa ccggcagcag acaaaggttt gcagtgtcac
    30601 atactaatat ttcaaaatca aaattctgcg gatttcctga tgcaatacat tctatgttca
    30661 tagtctctcc tctgtaaagc ctgttatccg gactgctgtt tataaaaccg gtaatattcg
    30721 gacgcatatt agtttcagtt ggaaataaac ctgtctcgac tccaaaccaa tagaaaagcc
    30781 ctgcgacagc tcttattgaa tgattgaaac aataatttgc ttccatatta aggtcaggaa
    30841 tgtttagtgg aggacttccg agaatttgat aataattctg aatcattgta tttgtaccgc
    30901 ccggaagaaa gttatttccc aaatgctgtt gcggaatttg tgaacagctc ggtccactcg
    30961 gaaaatgatc ggcaagctgt gaagttgtat atgcaagata tcttaaagga tctgaattat
    31021 cagcatatgg attaataaat ccgcctgcat tctttgctgt ctgccagttg taattaagat
    31081 atgcaccatc atcaataaaa ttatgataac aatcaccgcc gtctagttct ctgacatgaa
    31141 cgtcgttatg tgcatgcgcc ggcacagaca gatcctgaag aagatgagac attctgccga
    31201 gtacttccca tacaactgca ttaaaaacat tttccggagc agtgatttcc tgtctaacta
    31261 aagtttgtct tcctaataga tcataaacat cttccagcca tatttttttt gtacggaaaa
    31321 aatttatcag tccgcgtgta tgatagctgt atctgtatac aaaacctgaa tgatgaagat
    31381 aatttatgta ttctctctca ccggaatctc ctgtccattc gaaccattgt ccgtctctga
    31441 atttatttaa ctttgtataa gcatttgggt aatgaccaat actatttaga taagtaagag
    31501 tattgtcgcc gttcgttctg tcatcggcat tccagaaatg agtattactc acatacaaat
    31561 tgccgggtcc tctgacatca taaacaacat cttccttatc ttccctgtat gcgccagtag
    31621 taatttttgt tgtctgccat gcacgtgtac cctcatctgt catatcgcca atatgctgat
    31681 cgaattttgt ataatcaaaa tttggatatt tatcttttaa aagctgaagt gcctgcatgc
    31741 agatatacat gtgcacgttt tcagcataag gattttcttt tgaaactgtt tggctttgct
    31801 cagatgaatt tatttttttt tcggctgtgg cttgtgtagt ttgaaaagcc ataaaaagga
    31861 ttacaaataa aactaataat gtatgcataa taaataattt aatggtttat aaatcgtgtc
    31921 ttaaagattg cgttaataaa ttcttttgat aataaatccc tgtcagtaaa taatgtaagc
    31981 cattattatt atgccgttcg cagcggattg cggatgtctt gagttgaatt acttagattt
    32041 cgaacgatct tttaaaacaa tttcaaatta caaatttcaa attataaata attttaaatt
    32101 atcattgaac attgaaaatt taaaattgaa cattaattag gtgctcttat aaaattcata
    32161 tcaacctcct ttaatttaag tttgaaaaat tctctatcat attatccttt gaaaatctaa
    32221 gtgcaaatta tcttcttaaa tttgcatata caaattatta taattttatc gataagtcaa
    32281 attgcaattt taggtgattg tgaaaattta agatcgctgt tgcgggttgg atgaagttgt
    32341 ttatagaaat aaatgacagc caaaattaaa aaagtcagtt gctacaattg cagcagcaat
    32401 ctataattgc ggaatatttt aattttcctt tgaaaaaagt ttgtatgtaa tatacagtaa
    32461 acctttctga cattgaaaat ctattgaaca tgaatattta atatttaata tttaatattt
    32521 aatatttaat attgctcttt catggcttta ctgagctgat aaattttact gcattatttt
    32581 ctgcgatagc aacatcactt acatctgtaa acatatcacc tgtaatatct gaatttacgt
    32641 atcctttatt aaatacagaa acatcattgt caatatcaga caggtcagaa atatcgatag
    32701 ttccgtcctg atttacatcg ccactatata tgctgtatct tgatcctgtc agagtcagat
    32761 tatttccgaa tgcctgtgaa tgctgtgaag taagatcata atgaagtaca tcattttcct
    32821 ttaaattttc accacccgtt ttactccatg tttcaataga atttctatgt ttgattttaa
    32881 gataataagt gccggcattt acattataaa aattaaaact tcctgttaga tttactgagt
    32941 ctattacaga ctcggacgaa tcgacaatac tgtagggcga atttatatct ctgagataaa
    33001 tcataactgt atcatttatt tttaatttat cctcatcgat attataaaat ccctctactg
    33061 ccattttcag atttaatgta aattttactt catctctgaa aactctgtat gtgaaaatac
    33121 cggcaggcag tgacatttcg agagcgatat ttccttcggg agtaacttca ctaatattcg
    33181 gatgagaact accccagcca ataagtgtgt ttccattttt caatctctgc actgatccca
    33241 ttgcaaaacc aaaattatca ggtgtatttc tgtattgcca gacgagggtc gcagttttat
    33301 ttacttcatc taaatcatat tccacagctc tcgaaaaagg aggtgagtga taattaccat
    33361 tatcaaacaa agtaacatta ccattatcta atcttctgat atcatgctga tagctgaatt
    33421 tgatgggatc atttataaat gtaaactcat tattcttacc gcccatcctc catattatat
    33481 cacctgtatt tctgttgatc tttgtgatct cactaatatg tcttgaggag ataagcaaat
    33541 gtccgtcgtc atcaatatcg attgcattgc catgaacgta atctataagc gaatctgtaa
    33601 gattaattcc gatagcatct gttattaaca tatgatccca gcttctccat tgaaacacaa
    33661 cattattgga ttcatcaatt tcctgaatga caagtcctgt cacaatggca tttggatttc
    33721 cgcccggtac tatcatactc atatccacat gctgcggatc atagctcatt aaaaaagcat
    33781 gtccgtcatt tgtgatttga agttcatgat gatcagtaga atatccgtta ccgcaaaaat
    33841 atgacctgat ataattatga tcgttatcct cagcatgata accaagacct tcgaaaaaag
    33901 tctgcattcc gtttggctgt cttttataat ctaatgccca gacatacact tctctgaaat
    33961 aagaaatatt tccattatta tcagactgaa gtagatacgg tgtatttgga atttgagaaa
    34021 atggaaaatt tccaagaaat aattttcccg cagatggatt atttaaaatc cttaccgaaa
    34081 tattaggcag atccggtgaa ccttgcattt gtgaattata aattaaatct gaattaccgg
    34141 tctcatttaa aatgctttga ttgggaacgt ccagcggttt acttttttgt gtataaaaaa
    34201 tatatgaaaa attatttata aaacctgata aagttttcaa tcggattaat ttaacttcta
    34261 cctcttcatt aaatgcaaat ggttcacttg gaataaacag aaattttctt ttgtcttttg
    34321 ttaaaatgac cttaccgcta tgaataccgc tgaccgatcc ttttacaatg atagttttta
    34381 aaagatcact gctttgaatg atctcatcaa aaccgattat gatattatta ttaatgctga
    34441 cgagttttgc atttcgaacc ggttctgtat aaatgatatc tccttttgca aaattaacag
    34501 atagcaaaaa gataaatatg ataaaaaagt gatgcattta tttgatttaa aattttgaaa
    34561 tatttggggc tcttccataa ctttttagtg gtgataataa tttctttctc taaaatgcaa
    34621 atatcggaat aaaaaatcaa tagattggtt atgcaggtaa attccagcag atcttagttt
    34681 tcaaaaagtt tgtgaattta aaaacttatt atgaatttta atccactgca atttttttgg
    34741 caccaacaca tgagatcaat ttcaaattac taattactaa ttactaatta ctaatttcaa
    34801 acagaaagat tttattttta aaatggaatg tataaatcag tgtaatcatt ttaattagcg
    34861 taatctgcgg ttcagacaat gaagagacac taatttcaat ttacaaatga catattgttt
    34921 tatataaatt taatacaaag tatatagaaa ctccctgcta aatttgttaa ttttgccatt
    34981 attaaatttt ccggaaacag cttaaaaatg gcaaagaata cagatactcc aaatcccaca
    35041 aaattgcggc ttgaagagat agataaagaa cttgcagaat tatctgaaaa aagaaattca
    35101 ctggtatccc actggaatct tgaaaaggat ctcataaaat ctgtgcgtgc atcaaaagaa
    35161 gagatcgaaa atttaaaaac tgaatctgat aaatatgaac gtgaagggaa tcttgcaaag
    35221 gtagccgaga ttcgatacgg aaaacttctt gaattacaaa agacaattga tacaaaatct
    35281 gaagaacttg ctatagttca gaaagataat aaaatgctga aggaagaagt caacgctgaa
    35341 gatattgcag agatcatttc aaaatggaca ggcattcctg ttacaaagat gctcgagagt
    35401 gaacgtgcga aacttgttca catggaagag aatcttcagc aaagagtcat cggtcagact
    35461 gatgctgtgg ttgcagtttc aaatgctatc agaagatcgc gtgccggact tcaggatgaa
    35521 gatcgtccga taggttcgtt tatttttctg ggaactacag gtgtcggaaa gactgaactt
    35581 gcaagagctt tggcggaatt tctttttgat gatgagaaag caattgtaag aattgacatg
    35641 agcgaataca tggaaaaatt ttctgtgtct agactcatcg gtgcgccgcc cggatatgtc
    35701 ggatatgaag aaggcggaca acttactgaa gcagtgagaa gaagacctta tgcagttgtt
    35761 cttcttgatg aaattgagaa agcccatcct gatgtattca atattttgtt gcaagtgctt
    35821 gatgaaggaa ggcttacaga ttcacaggga aggacagtta attttaaaaa tacgataatc
    35881 atcatgacat ccaatctcgg ctcgcatctg attcaggaga agatgcagga tatgactgat
    35941 gaaaacagag atgaagtgat aaatgaatta cgtgtgagtt taatggatct tttgaaatca
    36001 actataagac ctgaatttct gaacagggtc gatgagataa ttgtattcaa gcctttgaca
    36061 acaaatgaga tcagagctat tgttgatatt cagatacaat atctgatcaa taaaatggaa
    36121 agaaataata tgaaactggt aatcgatgaa gaggtaaagg actggcttgg taaattagga
    36181 ttcgatattt cattcggagc tagaccactg aagagaacga ttcagaagta tgttacaaat
    36241 gtcttatcag aaaaaatact tggagctgat tttttaccgg gtgatacaat cgaagcaaaa
    36301 cttgacaaga gagggctgat agaatttgtt aagaaaaatt aatttcatta aaattccttt
    36361 gtcagtaaaa ataaataaaa cagaatattg ataaattaca ctgacaatta ttttgatctg
    36421 gagatattaa aaatgtcccg gatcaaactt cacgaagcca cagaaagtat ccgtttaaga
    36481 aatattttca atcgaatttc ccgatccaaa tatcttatca atcctgtgat cacaggcaga
    36541 tataatgatg atataattct tctcgacgga gctaacagat acggatcatt aaaagagata
    36601 ggttgtaagt tgatactcgc gcaggtactt gattataaaa gtccggatct tcagcttgac
    36661 aaatggaatc atcttgtcta tgatttaaat ataaatgaga tcataaattt ttgtgaaaag
    36721 aatggattta aatttgagag aacatcttat tcaaaaggta taaagaaaat taaaaatgat
    36781 tttcattata ctctcgtttg tgatattgaa aaagatgaga atattataat tgaactttcg
    36841 aaggacctgg gagaaatgat ggtacagctt cataatctga caactttcta ttttaagaat
    36901 tatgactttg acagatctga aggtgatata aaattttctg atattaaaaa gtacacccga
    36961 aggaatggag tactcatagt gtttcccgat tttaagaaag atcacatttt gaaaattgct
    37021 gacagtaaat ttcatctgcc ggcaggaatt tccagacaca ggatcattaa cagagtactg
    37081 catgtaaaat atgagatcag gaaacttctg agcgacaaga atattgccca aaaacaaaaa
    37141 gagttgaaag aattacttaa tgataaaatc gacaagaata aggtgagaca atatcaggag
    37201 tctgtaattg tatttgatga atagactaat tataataatt ataaaactgt ataaatttaa
    37261 ttgaagcccg agtatttcat agcgcggaga tatttatttt ccaaaaaaaa attcaatttt
    37321 ataacgatca tttcacttgt ttccattatt ggtgtttgca tcggcgtagc tgcgcttata
    37381 attgttttgt ccgtattcaa tggtttcaat agtaaagtta ctaatattct cgtaagtttt
    37441 gatccgcaca taagagttga atctgctgac agttctaagc tgactgatta ttccgaagtg
    37501 cagaaaaaaa ttcaggaact tggcatcaaa tctgcaactc catatacagt caataaagga
    37561 atgcttgcaa atgaagagat gaatgaagtt gtgtttataa aaggtgtaaa ggaaaatgaa
    37621 attgctgaag tatcaggaat taaagacatg atcaaacttg gtgaatttga tctgacgaac
    37681 aaaggtgaag ccggaggaat agtcatggga ttttctcttt tggggaaaat gcgtgcacgt
    37741 ccgggtgata ctttgacttt gctgagtccg gtaggattgg aaaatgcgct tacacagttt
    37801 gttgagccaa agactaagaa gcttatcatc acaggagttt atgattctga taataaagat
    37861 tatgacggca gttatgctta tatttcttta gaaaattctc aggagatatt tgaccttggt
    37921 aacagagtga acggtgtaga aatgagacta agtaatataa atgattcaga accggaaaag
    37981 attaaaattc agaatgctct cggtaataat tttaatgtac tgacatggta cgacctgcat
    38041 aaagattttt attcaatact taaagtagaa agatgggtag cgtttacaat acttagtatt
    38101 ataattgctg ttgcatcatt taatattctc ggttcactga caatgacagt cattgaaaaa
    38161 aagcgtgaca tcggagtttt gaaatccatg ggtgcgacaa ataagatgat cacaaatatt
    38221 tttatcactg aaggcttatc agtcggcatc attggaacag taacaggaat gattctcgga
    38281 ctgggaatcg taatagctca gatatatttt gaattttaca aactcgatac atcagtatat
    38341 aaaatggaag cacttcctgt ggaattaaga tttatggacc ttgtgtatat cccgatagcc
    38401 gcactactgc tgtgttatct tgcatctatt tatccgtcta aaagggcagc aagattaaat
    38461 cctgtaaact cgatcagatg ggaatgatca attgcaaatc agatttaaat cagatttaaa
    38521 tcagaaatag ttaattaatt tatgacaaca gaagatccaa aagtattaat aagtattgaa
    38581 gatttaaata aatcatatcc acttgcaggt acaaaaaagc ttgatgtact gaaagggatt
    38641 aatttaaata tttataagga agagatatgc gctatcacag gtaaatcagg tgcaggtaaa
    38701 agtactctgc tgcatattct tggaacactt gatcagccgg attcgggaaa agttttgttt
    38761 gaaggtgaga atattttcac caaaaaagaa aagcagttgg cagaattcag aagcagtaaa
    38821 atcggattca tatttcagtt ccatcatctg ctgcctgagt ttactgcatt agaaaatgta
    38881 atgatagcaa ctatgataaa tggtaaggat gatgcaggga aatcttttga gatacttaaa
    38941 gaagtcggac ttggtgagag agcagatcat aaaccgtcgg aactttcagg tggagaggct
    39001 cagagagttg cgattgcgag ggcacttgta aattctcccg gactgatact ggcagatgag
    39061 ccgacaggaa atttggatac cgaaaatgct gattcaatta tgaatttaat ttttgatctg
    39121 agagaaaaat ttaaacagac atttgtaatt gtcacacaca atgaagactt tgcttccaaa
    39181 tgtgacagga taattaaaat gcgcgacggt aaaatattat gataaataaa acagacatat
    39241 atggaaattg atggtgcaaa gattgtaacg gaaaagctca atctttacta cggtacaaac
    39301 cgtgcattaa aagacgtttc aatagagata ggagataatc agatcacagc tctcatcggt
    39361 ccatcgggct gcggcaaatc tacttttctc aggacactga acagactcaa tgatacaatt
    39421 gataatgtaa aattagaagg tcttgtaaaa attgacggca ttgatattta tgataaacgt
    39481 gtagatgttg tctcactaag aagaagagtc ggtatgatat ttcagaagtc aaatccattt
    39541 ccaaaatccg tgtatgaaaa tattgcttac ggtccgagaa taaacggtgt gacaaataaa
    39601 aaagagctgg atgacattgt cgaaagtaat tgtgtccgtg cggctatctg ggatgaagtt
    39661 aaagacagac taaaagattc tgcaatcgga ttatcgggag gtcaacagca gagagtttgt
    39721 attgcaagag cgttagcagt aaatcctgaa gtgcttctca tggatgaacc ggcaagtgca
    39781 ctcgatccga tctcaactgc aaagatcgaa gagcttatgt tcgatctcaa aaaaaatctg
    39841 accattgtaa ttgtaactca caacatgcag caggcggcaa gggtcagtga taaatgcgca
    39901 ttctttttta caggtgagat agtcgaatac accaagacca gcagcatgtt tacaaaacct
    39961 gagaaagaac aaactcaaaa ttatatttcc ggaagattcg gataatctgt aaatccgaga
    40021 taaagtattt tgattttaat aaatgaattc ataacttaaa taaaaattaa taaataaaat
    40081 atgcttcact accttgaaga agagcttgac aggctgaaag aaaaaataat aaaaatggga
    40141 agtcttgtag aagagcagat tgacttttct tttaaagctt tgtttaacgg tgatctcgag
    40201 cttgctcaga cagtgatcga cagagatgac gaagtggata aatttgatct gaagatcgac
    40261 aagcattgcc agagaatatt tgctcttaca caacctgttg cttttgatct gagacttata
    40321 atgtcgtcat taatgataaa cagtgacctg gaaagaatgg gagatatttc tgtaaatgtt
    40381 gccgagagag taaaagtact taaaggtcac gcagaactgc taagaaaaat gagagtaaat
    40441 gaaatggcag agaaagtcag gaagatagtg aaaatgagca ttgactgttt tgtcaatgga
    40501 gatgcagagc ttgcaaagag tgtaataaat tttgacgaag aaattgacaa tcttgacaga
    40561 ttaattttca atctgcttac tgaagaaatg aagataaaca ataatatcat tgtcccctgc
    40621 tgtcacatac tttcattagt tacaaatatt gaacggcttt ctgatcatgc gaccaatatt
    40681 gctgaagatg taatttttct tatagatgca aaaatggtaa agcatagtaa ggatcctgaa
    40741 aatctgaaac cgaaagaata aaatgtataa atgaaatatt taatcacact attatttttt
    40801 atttcatttg ttaatgtaca ttcacagaca atcaaatctg actcagtaaa gaaaattcct
    40861 tatgaccata acaggattct aaatcccaca gaactatcac ttcagtctga tattttcagt
    40921 tacgattcac tttatatatg gagcgataaa agaacgctgt cagagatcat ggatcaacga
    40981 cccggatttt ttataaataa tttcggactc ggcagcagga ataatttaaa ttataacgga
    41041 aacagttatt atcaaacagg agtattccgt gacggcattc aggtaaatga caattattat
    41101 caggggtttg atattcagaa tttttcgata agtgaaattg ataaggtaga agaaatttca
    41161 gcagtctcat ccttttttta cgggataaac agttcatcca aaagtgtaaa tgtaattacc
    41221 aaagatattt ttaatcctca gcctttttcg cagttcagat attcacagga cagagatggt
    41281 tcgttatatg ctgatgtatt tctgacactg cctgtctcac gaaaaggaaa tgtaatgatc
    41341 ggtcttacaa aacattcact cgacggattg tatgcaaatt ctgcgttcga tgtatggcgg
    41401 ggaagagcga gagtgaattt atttctctca ccaaaggtaa atctcagatt agattttaac
    41461 atgagcaact ttgaccgcgg actaaatgaa ggtctgatct attcaccagt taaagattca
    41521 cttcagaatc cgatggaggc aatggtcgta gatccgaatc agacagaaaa tcttgagaat
    41581 tatttttaca gtgcacagct tacggcaaag ttatttaaga atcaggactg gcttacaaaa
    41641 attaaattgt atagtgtgaa ttctttgaga acattgtata atgaacgcga tacaagtttt
    41701 gtcaataact acaatctgga ttccgggtat cagcattcta tattatatgg taccgagctt
    41761 ttacaaaata ttactttgaa ttccggcaga cattttaatt ctgatctgac ttttggcggg
    41821 aacgtttatg gaaatttatt taatggaaat ttcaacagtg taattgataa ttattttaga
    41881 aataattctc agtatctata cttttcattg aaagctaaat attatctgaa ctataaaata
    41941 ttttcagcaa caacgttaat acgtaatgac aatttttcag aatatggatt tctaaactac
    42001 ggtgcggaag gttcaataaa agcttttgat aaaaataatt tccttcttaa ttttaacgga
    42061 ggatatagac gaactgaata tgaacttttc aatgcagcta aaccaaaatt attatctttt
    42121 gaagaacctc ccgtttcata tacagttaat aatttttaca atgcaggtct ttcttttggg
    42181 tataagcaaa gtgaattata ttttgaatac agctcaggta aaaatcaata tgcaaataat
    42241 tctatacaaa atattaatgc aggtataaat ttaatttcag aatattttga tgtaatggtc
    42301 aattataata attcaaatga tcttctcgtt ccggaaaatt atatcaaatc agatctggca
    42361 ttcaaggata ttttattcag aggaaaactt aaattacgga caggattcaa tttcaaatat
    42421 tataacatca ggtatattac agatcagtat cagtcggttt attcatacgg cgtaacaaat
    42481 aacacattcc ctcagcagaa tcagtacatg gttgattttt atgtaggtgc aagaattgga
    42541 agggcaaata taaatattac agttgcaaat attttaaata cattagttta caatgcatac
    42601 cttttcccgc tggatgacag aggcggtctc ttcaatgcaa tttcgaggtt tacgatagtg
    42661 tgggatttta taaattgagg attaatttct tcatcccgca gagttcgaag agcacgtgac
    42721 tctgcggata cattctctga ttactttctt atgttctctc ttttttcggc tacaatcccc
    42781 ttggctctcc cttttttaag ggggaattca attatagatt ataatgaatt gtgctcttct
    42841 tatttgtaat ttttaatttt taactgctct tctcaaatgt ttttaacggg aatccacacg
    42901 aaacttatct gtagtatctg aacatggatt ttgtaagatt ggaaattatt aggattaaaa
    42961 gtaatattta tctttaccag aagattccgg agagcattgg actcttaaac aaaaatggtt
    43021 ttgttaatta tatttttaaa taaatttttg tagatttatt ttaatataac ctatgcttat
    43081 gtttcgactt attttaatct taatgtaaag ttctgatttt agattattat ctcttcggat
    43141 ccaatctttg atttttattt ccggtaaatg cattttaatt aaaaaatatt tacacaacta
    43201 aaaagctatg gaacttacat catatattac aaaacctaaa tctatattta tgaaatacat
    43261 tttaattatt acgatctctt tactatcttt cagttttgca aaaactgaaa ttatttacaa
    43321 tgatcctctt cccaatgcac agcttgtcag cattaataac aatatagtaa tcggatttga
    43381 tgagatcatt ctaagcagtg atctgataaa aaatatatct gtgaaaggat ctgccagtgg
    43441 gttacacagc ggtaatataa ttttaacaaa ggacagtaaa aaatttcttt ttataccgga
    43501 tgaaccgttt gcttttaatg aaaaagtaga agtcaaaata aaccggctta gaacttcttc
    43561 aggaaaattg aataattatt catattcatt ttatacacaa aaaagtaaac ctgaatataa
    43621 tccggatcaa agctttttaa gtgagacagg aaattcaaat gttattttcc catcacaaaa
    43681 ttcaggttca tctaatcttc cggttcttac tgtaagtata aataataatc cttcacccgg
    43741 aaaattattt ctcggaaatt ttccttttaa tgcgattccc aatactccat atatgattca
    43801 gtcaggtaac aatggtaata tttcctattc cagagaattc agttccaacg ttttggatta
    43861 taaaagacaa ccaaatggga tgatgactta cttcgaagga aatgcatatt atggtgagga
    43921 taacgatcac aacctattaa gaacttttca atgcggtaat ggatattcga ctgaccatca
    43981 tgaacttaaa gtaacaaatg acgggcatgc atttctaatg agctatgatc ctcagcaagt
    44041 ggatatgagc aaggtagtac cgggaggaaa tcccaatgca gttgtaatag gtcttatcat
    44101 tcaggaaatt gatgaatcca ataatgtagt ttttcagtgg aggagctggg atcatttttt
    44161 aattactgat gcagttggcg taaatcttca tgctttgact attgattacg ttcacggaaa
    44221 tgcaatagat attgataacg acggacattt gctgatctct tcaagacact ttagtgagat
    44281 cacgaagata aatagaaata cgggagatat tatatggaga ttgggcggta tcaacaatga
    44341 gtttactttt acaaatgatt cgatcaaatt cagttatcag catgatatcc aaagaataga
    44401 taacggaaat ttaacgttgt ttgataatgg caactttcac tccccgccat tttcaagagc
    44461 tgtagaatat cagatcgatg aagtaaataa aactgctact ctcgtatggc aataccggca
    44521 gactccggat aaatttgcta ctgctatggg ctctgtacag agattaaaga acggaaatac
    44581 tctcatcaac tggggaagtt caaacccaaa tatatttgaa gttacacccg atggaaatat
    44641 tgtacttaga atgtcactgc ctacggcaat ttttacatac agagttttca gagatgaagt
    44701 aaaatttact ttaaatctga aaatggcagt cgaaggatct tataataaca atcaagataa
    44761 actaaatatt aatgatactg tcacagctta tcttagagaa ttaaattcac cttataatat
    44821 tgtcgattca tcaagatctg aaattgattc agtagatctg aaaggaagtt ttaattttta
    44881 taatgttaac acaggttcat attatataaa aattaaacac aggaattcaa tagagatatg
    44941 gagtaaatct ggtggtgaaa atattaatga aactgatgta ctgcattatg atcttacttc
    45001 atcacaatca caggcatttg gaaataatct gactctcaaa ggatcaagat attgtatctt
    45061 tagcggagat gtaaatcagg ataggactat tgatattttt gatctatctg aaattgacaa
    45121 tgatgtttca gtctttaatt caggttacat taattctgac atcactggtg atttgattac
    45181 tgatttaaat gatgttgcta tagctgaaaa taatgctttt cattttataa gttctgtcgt
    45241 accctgatga aaagcaataa taaacttgaa ttttagaatt tgtaatttgt aatttgtaat
    45301 tgctcttcta aaaatctgtc attacagtag attgactgat caaatagtta ttatatcttt
    45361 aataaattca aaatcacctg catcccttct gtccacccag ttaatatcgc ctgtggattt
    45421 aaagatataa gtccttccgg taatgctgcc gttgatcaca agtggatctt tccctttcag
    45481 tttaatcttc accatgttta taaaatgtgg atcagtattt accgaaagtg acattctttt
    45541 tttattacaa ttacacataa tattttttat tcaaaatttt tgttggtaaa tttttctaaa
    45601 attattgctg cacctgaata gtacagacac agtataatta tctctgcaaa gccaattcca
    45661 attataattg ataacaacaa tgagacccag atactcaaac agtaaaaaca atccatcagc
    45721 tttccaaaaa atcccgatcc aagtagtttt cttattctaa atatcagatc aaatggtccg
    45781 tcttccatgc tgaataagtg agtgatccgc cagacaacaa ttattattac aaaatatttt
    45841 acaatatcag gcatctctcc ggttttaatt agttgcaaat aatcaactga cacgggttct
    45901 aatattagat tatttaattt taaaaaaaca gcagggaatt cttttgggta aattccctgc
    45961 cggtagaaac tgtcccgttc aaatacagga cagctcaatg tattctattg cagacaaata
    46021 ttataacctg tccacacatg gaatccgaca ctctgactgt ttacaattgt tttatcagaa
    46081 gcatatagtg aaatattccc gcacggatta cttgatgcat ttgtcggaat gctgaaactg
    46141 ttacccggta gcggcagtgt attggaagtt ccgctgacac ctccaccaaa actgctgccg
    46201 aaactgaatg tatgaatgtt agcatcatgt acataataat gtcctgtgat agtatcacct
    46261 ttgtgataat gtgtacagtc accaccgtca tctaccgaca atgctatttc cggagcaatg
    46321 ttatccattt ttatattctt gctgaatgtt ccgaaaacac tttcgatctc cagtgaaact
    46381 tccagcctgt catttgtacc cggattgaat cttgccagaa tactcagagt gttttgattt
    46441 acagttaaga aattataata cccgaaagca tcaggagtct gtgtagtgta ctgaacatat
    46501 ggtgcaacag gcagccatcc tactactgta aaagaattgc tgagataaga agaaacattg
    46561 gtaacagtat ttcttacttt tattctgtat ctctcacccg ggaatgtcgg accgtttaca
    46621 acaaccactc cgccgaaagg tgatccggaa ggaacaggaa gtccgttaaa tgcaaatgaa
    46681 gctccgggtg tagtaagtcc tgtaacatca tttattttat caacaggaat tccaccgata
    46741 atattataga gaggcattac tgaaccgatc tcagttccgg gttttatctg tacatgtgta
    46801 tcttctctgt tgccgtaaaa attaagatca tcaggatcag taactgacgg aagtgcattc
    46861 catgaaagaa ctccccttac tcttacaata tttggattct cacatatttt tttgtgcagt
    46921 gtagtatcaa gcggaagagt cactgaataa aataaaccac cggccggaat gtcgagatca
    46981 tgaatatcca gctctactgt attcagatat tcccatttac aatcatcata ccagtcaatc
    47041 cagaatgcaa catattcctt gctgccgtca tcacaaagat caccgctgta accgcttttc
    47101 tttttgatct ttatagtggc tacaagactt tccgtattat aatccagacc aatgcattca
    47161 agctcttccc attcaacatt agcctttgac ttatctttta aaggaaatgc agaaaattta
    47221 tcaataagtt ttgtataatc aattttcaga tcctttagaa ctttctgtga atttagtgtc
    47281 agctctgaca tgggatactt gatcatattt atggctgcct tatatgcaaa cctggacgga
    47341 tctatgtcag cttttttata tttgctgtta agctcggata atgaaagtgt tttggcaaca
    47401 ggtttcagat cgtcaatatt aatttccgaa acttcagcaa gctgttttaa ggaaatatca
    47461 ggagtgagtg cagcaagatt aaataattcg ctgtacttta gtatagacag atcatcataa
    47521 aaaaggaaat aaggtttaag ctgaatatca gcttccttta catttcccca gacaggattc
    47581 catgaaggag aatctgcagg cggttcaata ttccatgata atatagctct tacccttggt
    47641 aatacaggct gactgcattt tgaatacttg cttgtttttt tctttaaaga agcagtatag
    47701 gtcagcggca tagttgactt cttgttgcag tctgtatctg ccggaatatc atgaacattt
    47761 actgctgtta cgccctgatc tttccagccg gctccgtcat gaaagtttat ataaaacctt
    47821 acatattctg tagatcccgc tgtgcataga tttccggaat atccataaga tcttttaata
    47881 ttaaaagtag cttcaaggat ctgtgtggaa ggattatatc caatacatga aatttcttca
    47941 taggataaat ttcctatcat tttgaaattg gatttaattt tactttccgg gatgttaccg
    48001 aaataattcg gattctttgc gagcatcata aggaaattgc tgcgctccgg ctgaatcgtc
    48061 agccttgctt tgcctgtctt acttgttttt agttttttag ccatttttaa aaaattaatt
    48121 ttgtgcctac tctttggagg attttcggca gcttcctgtt agtcttaaag tggaagtaaa
    48181 ttttccggat gagttgtaat aatttactga agaataacat tacaaaatta ctattagcag
    48241 atagaaaata gaatagggag gaatacgctt ttttaataag cggaaacact tatttaattt
    48301 caaattttaa attacaaatt tcaaataggt tggcttaata atgctcttta aataataaat
    48361 ttactaaatg gaaaattttt ttatttttgt aaattgaagt ttgatcgaaa ttaaaatcgg
    48421 gcactgtaat atttaatttt ttaaggggga attcactttt taattataat gaattgtgct
    48481 cttctttttt gtcggtaaat ttcagatcaa tttattcgca gcttttaaaa cttcttccac
    48541 actgatattc ttcatacact taaattcatc ctcacccgtc cagatacatg ctgcaggttt
    48601 tggggagttg taaaaacacg gactgcattc gagatctttt ctgacaattc tgtattcagt
    48661 cttccatgga tacaattctt tataatttgt ataagcaaat attccaacta ccggtaactg
    48721 caaagccgct gctgtatgca tgaatgcagt atcattggaa ataaataaat tacaatgctt
    48781 tatccttgca actgaatcca tataatctga agtagatgca atgtgtgaat gacttccaat
    48841 tttatcttta atttcattgt tcagatcaaa ttcatttcca aataaaagta tctctgaatt
    48901 atgttcactg attagttctt ttccaagctt aacatattta tccttatccc accttttatg
    48961 aatatgattt ttcaaaactg atgaacccgg atgaaagccg atcagcattt ttccttcaaa
    49021 attattttca gaaatccatt tctctgcagc agacttatga ccttcactta taaaaatttc
    49081 cataggaccg gcgtcgtcat cttttacttc actgagtatt gattttatca gatcaagatt
    49141 ttgcagcacg ttatgtctgt ctttgatttc attgacgaga atgtcattca taaattcagc
    49201 acgcgaaaga gttgtatgca gatagtgatg tgcaattttt ttctttgcac cgaggaatgc
    49261 atttacaaca ttgtattcga gtctgttggc agggtaaaca tttatagaaa agtcgaattc
    49321 atttttacta agctttctaa tttcagaaat agattttatc tttgactgct tcataagatc
    49381 tataaaatga atttcagaca ggtaaggact ttcagcatac atttccttta cagatctgaa
    49441 catcacgagc atatctattt ttgcatcggg aaatttatct ttaagaattt ttaaggcagg
    49501 tgaaaacatt aaagcgtcgc cgttaccgga gagagcgttt ataagtattt tcattttagg
    49561 attttacttt agatcaatta tttctgttga aataaattat gggtcatata cattactatt
    49621 ttgtccggca acaaccccct tcgctccccc ttttttaagg tggaataact ttcggataca
    49681 tcggaagttg ttctaactaa atttattttg gagcttactg aataactttt taatacatac
    49741 tccggcatag aaagaaccaa aaaaaattat taaacagtta ttgctcctcc ccctttgtcc
    49801 aaagaggagg ttgggagggg tttgtaaaat tgagtttaat ttgaccactg tgtttaccgt
    49861 gttgagttta ttcaattcga tgtttgctgt ttcttactgc aacacacccc tttgctcccc
    49921 ttttttaagg tgg
//
LOCUS       DAORVQ010000005        45433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_222_length_45433_cov_6.134008, whole genome shotgun sequence.
ACCESSION   DAORVQ010000005 DAORVQ010000000
VERSION     DAORVQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 45433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45433
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_45433_cov_6.134008"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..86
                     /gene="ffs"
                     /locus_tag="PLX80_00960"
     ncRNA           <1..86
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLX80_00960"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            124..1131
                     /locus_tag="PLX80_00965"
     CDS             124..1131
                     /locus_tag="PLX80_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY19394.1"
                     /translation="MSKLKIGIVGLGGIAQVIHLPILSKMADVEISAVCDSNVSKCKS
                     ISSKYSVNKYFKDVEKMLEECEDISAVIVATQTSVHKDIAKKCLEAGKDILIEKPIAR
                     NYKEAKTIVDIAAKMKKKIMVGMNNRFRNDMMLQRTFTKAGEIGEIFYVKTGWIKPKS
                     SDQNWMLHKVLSGGGVFLDTGIAMLDLGLWILGFPEIKKVSATNYYHTTKSVEDSSIV
                     IVSFKNKATLTVEVSWGIQREGELFYCNAYGKSGSSSINPFRIYKKMEDNLYNITPKK
                     LPLPSNQFKKSYEYELQNFIGAVHGKNDLISSGKDALKVMQIADAVYKSAKSGKEIIF
                     K"
     gene            1184..1879
                     /locus_tag="PLX80_00970"
     CDS             1184..1879
                     /locus_tag="PLX80_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY19395.1"
                     /translation="MKLKILVVDDEKDIVDILKYNLERENEFEVIPAYNGKDALELAA
                     NIPDLIILDIMMPELNGYEVCKKLKSNPETSGIPVIFLTAKENEIDEIIGLEIGADDY
                     ISKPISPRKVIARIKSVIRRTSIDSEKLFKSDQKITFQNLEIDSQSHSVKIDQKDIFF
                     PKKEFQLLHFLISNRGRVYSREILLNQLWGENIYVVDRTVDVHVAKVREKLGEYSDYI
                     ETIKGLGYRFKDN"
     gene            1901..2986
                     /locus_tag="PLX80_00975"
     CDS             1901..2986
                     /locus_tag="PLX80_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY19396.1"
                     /translation="MKKVNLLTKYSLIYFSSLIILLILFSNLLNLKTDVIFISGIFGL
                     IMFFVSMIFFYFEISRPLKNILSISEKLKSGKDSDKFNNFEFKITDLKEILNKISEVR
                     NNTTGNREVHQLINEIHLIAERILKELELAKVFKVNRNEFMGNVAHELRTPIFAIQLS
                     LETLLDGAINDEKVNIDFLNRAFNQTKRLKELVEDLISIAKFETGLKMSKRYFGISNV
                     ISKTLEELEDIAETKKIQLKFDADPLNGIQVFGDEERLKQVLINLIDNAIKYTPEGGI
                     INIGVDVHEKDVIVKVQDSGIGIPEKDLPRIFERFYRVDKTRSRDVGGSGLGLSIVKH
                     ILEAHSSRITVESIKNEGTKFEFNLKR"
     gene            complement(2995..4422)
                     /gene="gatA"
                     /locus_tag="PLX80_00980"
     CDS             complement(2995..4422)
                     /gene="gatA"
                     /locus_tag="PLX80_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQY19397.1"
                     /translation="MNSPARNLSEIRKGLTEGNITCAELVQIYLKNINDQKDLNVFIS
                     LFEDEALLRAGEIDEKIKEGKAGKLAGAVISVKDVISVKDKKLTCGSKMLSEFETVYN
                     ATVIERLLSEDAIIIGKVNCDEFAMGSSNENSYFGPVKNPVDTSRVPGGSSGGSAASV
                     SANMCLLSLGSETGGSIRQPASFCGITGLKATYGRISRFGLVAFASSFDSIGIFGNNN
                     NDIAILLEVIAGEDDRDSTSSEVSVPAYSELSEKTFDPKEIKIGYAAEYFEEGLDPDI
                     KKGVLDKINLLREKGFIVNEISLPHTKYVIQTYYILTSAEASSNLSRFDGVRYGYRSE
                     NSETMEDMYVNTRSEGFGEEVKRRIMLGTYVLSAGYYDAYYRKAQKVRRLIQNDFENA
                     FQKVDFIISPTTPNTAFKIGEKIEDPLSMYLNDIYTASVNLAGIPAISIPCGKDRNNL
                     PFGIQVMSKKFDETGLLKIWNELIT"
     gene            complement(4509..4697)
                     /gene="tatA"
                     /locus_tag="PLX80_00985"
     CDS             complement(4509..4697)
                     /gene="tatA"
                     /locus_tag="PLX80_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQY19398.1"
                     /translation="MFGLGTPEIILIAIVILVLFGAKKIPDLMQGLGKGIKEFKKASS
                     DIEKDLTSTDDKDKKNSN"
     gene            complement(4735..5406)
                     /gene="purQ"
                     /locus_tag="PLX80_00990"
     CDS             complement(4735..5406)
                     /gene="purQ"
                     /locus_tag="PLX80_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015118665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQY19399.1"
                     /translation="MDHVKAGVIVFPGSNCDHDVYNVFRHILNADTKFLWHKETDIGD
                     RNIIILPGGFSYGDYLRCGAIAKFSPLMNEVIKFANNGGVVIGICNGFQVLCETGLLP
                     GALLRNENLKFVCKTVTLKVENNKSIFTGEYRIKEEIEIPIAHGEGKYYCDEETFISI
                     TGNNQIIFRYKDNPNGSLDDIAGIQNVKRNVLGLMPHPERASDEAIGNTDGYRFFKSV
                     AESLL"
     gene            complement(5407..5667)
                     /gene="purS"
                     /locus_tag="PLX80_00995"
     CDS             complement(5407..5667)
                     /gene="purS"
                     /locus_tag="PLX80_00995"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQY19400.1"
                     /translation="MFTSKINIRIRDTILDPQGKAVEHSLKSLGFDSVVDTRIGKYIE
                     LKIDAATEEEAKKITDDTCRKLLANPVMEDYEFSITKDGEGK"
     gene            complement(5692..6516)
                     /locus_tag="PLX80_01000"
     CDS             complement(5692..6516)
                     /locus_tag="PLX80_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19401.1"
                     /translation="MNDEEKYLEIREKLRSLETVHASSDFTNKLHLRIVEEESSRRKE
                     HIKKFDAEKGGFLRNLFNNRQYPWLIPSVGFAVVIFFVFYITFISRDNFTNNENEISA
                     DIKQETLNKDNTAELKKNTEQEKLQAELIEQDKEKVEEEKNGESSNENMNNEKKNGLT
                     QNSDLAFSDNNKKRISPSETKTENETDKPTENKTIPELKKESETNVTADVKTEVAKGN
                     EMATPKSRSKSDSEKIVIKRSKESISADESGDNGLSTKLDKINKAGLENIRNEIIK"
     gene            complement(6536..7144)
                     /locus_tag="PLX80_01005"
     CDS             complement(6536..7144)
                     /locus_tag="PLX80_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY19402.1"
                     /translation="MPGKSKTEYKILTDEELILLFQSEDVKAFNELVNRYKDRLVNFI
                     FRYTGSREDSEDLAQDTFLRLYRSKHLYVEIAKFSTWFYTIALNLTRTYMSKKGKMKK
                     KSISNYYEDEEKDYDLTSTYIHPDDSANAATEGEIIQRAINSLNEKFREVIVLRDIQD
                     LDYEEIAKVTSLPLGTVKSRINRGRECLKEILESLHKPKIRK"
     gene            complement(7281..8366)
                     /gene="holA"
                     /locus_tag="PLX80_01010"
     CDS             complement(7281..8366)
                     /gene="holA"
                     /locus_tag="PLX80_01010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQY19403.1"
                     /translation="MAKNSSNILSAKSISINKLCENAADYIDKNNIFFYMPDKIIADE
                     IIDNFYNAFTNNDPDRKNNLLHFNADDKVTESILNECSNTGLFGGRKVVVVRNVRKFL
                     KNDKIALADYIKRSNPDTLLILIEPDISTEINKIFDAELFLSKTNGEGIQNFSDRINL
                     FKPEEFSEDELIDWVVKKFDGYKISKDTVRHLLLFSNYSPGELLSEIEKLKTYCYSSK
                     EVSNDSVNLCNGIAKDFKETDFIKAVISKDSELAIKIYKKISLKKEVEVYLVILLSSA
                     FMAIFKLQDPSSSRLNEFNLKRELKLWSEEQLALLPLYQKTASSISSKKIRNIIENLY
                     NTDILLKSTGGVKFTKMFSLINNICAD"
     gene            8587..9903
                     /locus_tag="PLX80_01015"
     CDS             8587..9903
                     /locus_tag="PLX80_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19404.1"
                     /translation="MCFKTVKFCFFIVIIFYVIESGAEAQILKWRDLPNSPYNPGGRF
                     DDVYFINPDTGWIISISDVVRTNDGGNNWDTTFRIPGGNFRSIGFFDPQNGLLGTLSE
                     DSNKVLYRTTNGGVNYTAINNLPPPGLKGTCGINIVDENTAYICGRWFTPARILKTTD
                     KGISWTSFTIDTALANSFVDCYFWSADSGIVVGGYRTINFSAGKSAILMTTNSGVSWQ
                     RVYVSSRDGEYCWKISFINKNTGYVSTERSGFTNYLKTTNGGFNWEEKPFLNNYTVQG
                     IGFLNETTGWIGGFGGANYETTDSGNSWHIAGWGRQMNRVRFLNDSLGYAVGQRTYKF
                     SSEPVGITNISNEVPDKFQLFQNYPNPFNPATIIKFDIENEHDQHVNLIIFDALGKRI
                     STLVNEKLNAGTYEVDFDGNNYPSGLYFYKLQTNKHSETKRMILLK"
     gene            complement(10066..10788)
                     /locus_tag="PLX80_01020"
     CDS             complement(10066..10788)
                     /locus_tag="PLX80_01020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY19405.1"
                     /translation="MYKSKIAVYFSVVIIALVLMSCGDKKDSTETKTEEKKTTETKTE
                     TKTTETKTDTTGKTNSETQTSEKKDSTMENNETAGKEEKSADGSFVDMQTTMGNIKIK
                     LYTKEAPLTTANFKKLVNEGFYNGIIFHRVIDGFMLQAGDPTGTGTGGSKEIIKDEFG
                     PGLKHSKKGMLSMANRGPNTGSSQFFITLAPTDWLDGKHAIFGEVVGGMDVVEKIGKT
                     KTGPNDKPVTEVKITKATVVAK"
     gene            complement(10837..11862)
                     /gene="pheS"
                     /locus_tag="PLX80_01025"
     CDS             complement(10837..11862)
                     /gene="pheS"
                     /locus_tag="PLX80_01025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQY19406.1"
                     /translation="MLDKIESLKSEALKESEIINSDKSLEDYRLKFLSRNGILSRLFE
                     DFKNVDKESKGQVGKTLNELKISLQKIFDEKKSALDSENTADPDTPDLTLPGRTFNKG
                     TRHLINQALEEISAIFEKIGFKVYEGFAIEDEYHNFDALNTPEHHPSRDMQDTFYIDS
                     TGKDKKYLLRTQTSSAQIRIMEKIKPPVRIISPGRVFRNEDVSSRSLAFFHQVEGLYV
                     DKNVTFSELKGTLNYFATEYFGESLKTRLRPSFFPFTEPSAEMDVECFLCKAKGCRVC
                     KYTGWLEILGCGMVDPNVFKMVGYDPEIYSGYAFGMGIERTLMIKHGVPDIRLFYEND
                     IRFLKQF"
     gene            complement(11982..12329)
                     /gene="rplT"
                     /locus_tag="PLX80_01030"
     CDS             complement(11982..12329)
                     /gene="rplT"
                     /locus_tag="PLX80_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQY19407.1"
                     /translation="MPRSRNKVASHRRRKRILKEAKGYYGARSKVYTAAKNAVEKGLT
                     HAYTDRRLKKREYRSLWITRINAAARLNDTTYSKLINALNKKNIDISRKMLADLAYGN
                     AEAFTEVVKFALK"
     gene            complement(12364..12558)
                     /gene="rpmI"
                     /locus_tag="PLX80_01035"
     CDS             complement(12364..12558)
                     /gene="rpmI"
                     /locus_tag="PLX80_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQY19408.1"
                     /translation="MPKMKSNRAAKKRFKVTGTGKVKREKAYRSHILTKKSNKRKRQL
                     GKATLVSEQETRTVKRMIQA"
     gene            complement(12551..13300)
                     /gene="infC"
                     /locus_tag="PLX80_01040"
     CDS             complement(12551..13300)
                     /gene="infC"
                     /locus_tag="PLX80_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQY19409.1"
                     /translation="MKNKKYKERVNDQIRAPQVRVLDDEGNALGVMSSNEAMRIASSR
                     GMDLVEISSASKPPVCKIVDYGKFNYERQKKEKLAKKNQHVMNIKEIRFNPNTDTHDI
                     DFKTKHLRQFLMEGHKVKAYVTFKGRMITHPEIGRKLMDEIVEKLNDIGKLEASPRME
                     GKQLYAFFLPDKLKIQAIKDADAKEKKLEKKLAKTESKKEEDKETKNIESESIESEDK
                     ETKNIESEDNSTDNSESILEVNNNKDGKEDA"
     gene            complement(13334..15265)
                     /gene="thrS"
                     /locus_tag="PLX80_01045"
     CDS             complement(13334..15265)
                     /gene="thrS"
                     /locus_tag="PLX80_01045"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQY19410.1"
                     /translation="MSDKIKITFPDNTVKEFDKGISAYDIAASISQRLADEILAAEIN
                     GKMKDISLPLNEDAKVVFHKFDSDKGKEVYWHTTSHMMAQAIEELFPGARFGVGPPIE
                     SGFYYDIDSDRKFTEEDLKKIEDKILEISKRDLKPERKDIKRLDAIEFFKSKREDPYK
                     VEILETIAKDEDEVSLYSQGEFTDLCRGPHLPSTEKVKSVKLLSVSGSYWRGDEKRQM
                     LQRIYGISFPKQKELDEYLKNLEEAKKRDHRKLGKELDLFSFHEEGPGFPFWHNNGMV
                     LLNNLKEYLRAKLAELDYQEINTPIILNQKLWQQSGHYDNYKENMYFTDIDERDYAVK
                     PMNCPGSTLVYKTGMKSYRDLPLRLYEFGLVHRHELSGVLNGLFRVRSFTQDDAHVFC
                     TPDQIEEEVTILIKLVFEIYDAFGFDDVQVYLSTRPEKYIGSTEVWDNSEKALATALE
                     KAEIKYKINEGDGAFYGPKIDFVVKDSLRRNWQLGTIQLDFSMPGRFGLEYVGSDGNT
                     HVPVMIHRAIFGSFERFVGVLTEHYGGYFPLWLAPVQVSVLPIADAHFEYADAVSKKL
                     REKGFRVFTDKRNEKVGYKIRESENRKIPYMLVIGDKEVEGNEISVREHKKGDTGKFE
                     LKDFIEKINLQVLEKAGYN"
     gene            complement(15346..15419)
                     /locus_tag="PLX80_01050"
     tRNA            complement(15346..15419)
                     /locus_tag="PLX80_01050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15383..15385),aa:Val,seq:gac)
     gene            15620..16240
                     /locus_tag="PLX80_01055"
     CDS             15620..16240
                     /locus_tag="PLX80_01055"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HQY19411.1"
                     /translation="MKIFLISALLFLLICTSQSISQSNSPNPNAWAVYSGAVRTVAAY
                     GDYVYMGGDFKYVGPKTGCLVKVSANTILVDNAFPKVEVNVVTAVGDVNGGWYVGGNF
                     NSIGGVTRNNIARINSDGTVHPWNPNSNFIVNQIVLSGNDIFVSGGFSFIGGQSRTHI
                     AKLNKIQRVPLIFYGISPLLVLVSKASVTNNKRKLVVFRIKSFDFI"
     gene            complement(16436..17611)
                     /locus_tag="PLX80_01060"
     CDS             complement(16436..17611)
                     /locus_tag="PLX80_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQY19412.1"
                     /translation="METNSQKSSKPRIQSIDFLRGLVMVIMALDHTRDYFHQYHKAGN
                     DALDFGTTNEWIFLTRWITHFCAPVFVFLAGTSIFLYGSRGKSKNETAVFLLTRGFWL
                     IICEFTIVGFAWSFSLFVPRLLLGVIWVIGLSMVVMSALIYLPKKILLPVGLIIIFGH
                     NILDGYSGNSMFWSIIHEPREFIISGRRVVALYSFLPWLGLMITGYSFGNIYASGFDK
                     VKRKKILLSVGTVSILLFIFLRAENIYGDPGNWDTQKNFLYSVLSFVNTTKYPPSLLY
                     LLMTVGPCMFILAFTENISNKFSKIIIVYGKVPFFFYMLHLYFIHSAIWIFYLFSGNS
                     ITNMDFFSRNFNTPSGFGYSLGVVYLVWIGIILLLYFPCKWWGKLKSENKSPWLSYF"
     gene            complement(18135..19388)
                     /locus_tag="PLX80_01065"
     CDS             complement(18135..19388)
                     /locus_tag="PLX80_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HQY19413.1"
                     /translation="MKVIIFEDNKFKNLFPLNMLRASFDIKCGAVSIKERIINILCGR
                     YDVSLICRNSIAAMMSEINELRINEISKDDILFLNGRVIFSQESLEKIISEKKKNTAN
                     YFKEEVVSAYLTKDKIKEFKSYFERSFSEGINFEEFFKRAEVSKIDFGKNEDIFILKY
                     PWDAVKYLLQGGLTTDLEIYLNSKKKLKPTKRQDNFVNHKGIYVSSKTLLNPGSILDA
                     SEGKIVIEENCVIEPFCFIKGPVFIGKNTLVKSGTKIYGPCSIGKNSKVAGEIAESIF
                     HSYVNKQHDGFIGHSYICPFVNLGADTVTSDLKNNYSKIRINIGDQETETGMQFLGSI
                     IGDHTKTSINTMLNTGTIAGIFANIFGGGFPSKSIDSFTWNDGSSKPVPYILEKAVKT
                     AVTVMSRRGIKLSKNYEELMMLYSK"
     gene            complement(19472..19744)
                     /gene="rpmE"
                     /locus_tag="PLX80_01070"
     CDS             complement(19472..19744)
                     /gene="rpmE"
                     /locus_tag="PLX80_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQY19414.1"
                     /translation="MKKNFHPEYYKCDVICNCGENRGVLFTTRSTVPEIKVEICSLCH
                     PFFTGTQKIIDTAGRVEKFNKRYGKSKKAVDSKEAAENTETAETKA"
     gene            19970..21607
                     /locus_tag="PLX80_01075"
     CDS             19970..21607
                     /locus_tag="PLX80_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19415.1"
                     /translation="MIKKHPTVKFFLVCFTLLSFIGMKDLPSPKVTHDSNQNPNAPQV
                     ITSLRQNVNNVSTWFRTNGSFNRDPATGNSGFEWPKGQSKYARYASGLWLGGIVGLDT
                     LIAIAEYDYEYQPGYVDDAGNPQGRDNPNYRIFNFSDNDTSDFEAWRTIASLQGAYLN
                     SNGNPFRMGSQTMFYSITDGYPDSHGNTAGSTAPLKAVILQTNWSYINVNLKDVVFTE
                     YRIINRSNQAWTKFYISIWTDDDLGDAVDDAVGIDTSANMPLGYTYNFTNNDQEYGAA
                     PPAVGFTVLRGPVTESIGDTVRYYSPPGSSNLVVLPNMKEIKLSSFNTYINADPSVGD
                     PSNYRETYRNLQGLRRDGSQWINPNTNQASVYPYSGDPETSNGWIMPSGSDRRSLMSF
                     GPITIQPGDTQSVIVAQLIAKGSNNLNSITALRNLTSYVHGIYQDNFQSVVSVNNYSI
                     QTPDKYSLHQNFPNPFNPSTKIRFDIPSNAAGQPSNVKLVVYNSLGNEVRILLNEKLQ
                     SGSYETEFKGEDLSSGVYFYKLETDGFIQTKRMILLK"
     gene            21905..23962
                     /locus_tag="PLX80_01080"
     CDS             21905..23962
                     /locus_tag="PLX80_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQY19416.1"
                     /translation="MTDKKILLKAFASMCTSKKMAEIYDDNKQICKYVHSTSRGHEAI
                     QLAVGMQLRECDLVSPYYRDESILLGIGLEPYELMLQLLTKRDDPFSGGRTYYGHPSL
                     RRDGFPKIPHQSSATGMQAIPATGAAHGIHYLESAGLLNNSEDIENNPVIVCSLGDGS
                     VTEGEVSEAFQMAVLKKLPIIYLIQDNQWGISAYKDEMRAMDAYEFAAGFKGMKRIRI
                     DGSDFKESYTKFGDMLNYVRTKRKPVLIHAAVPLLGHHTSGVRKEWYRNDIDEHTKDD
                     PIPKLRKVLLKEGVSESDLLEIEKNSALFAEESFAKAVAADEPSFDSLTLHEFAPTPV
                     TEEKGERSPAGKESVIMVDAGLYAVREIMDKHPEAILYGQDVGARLGGVFREAATLAQ
                     RYGDNRVFNTPIQEAYIVGSTAGMSAVGVRPIVEIQFADYAWAGINQLVVELSKSCYL
                     TMGKYPVSCVIRIPCGAYGAGGPFHSDCIESSLLPIRGIKIAFPSNAADMKGLMKSAF
                     YDPNPVIMLEHKGLYWSKVPGTDAAKCIEPSEDYIIPFGKGRIFQNASEDKINSGESL
                     SIITYGMGVHWALNASKDFPGSIEIVDLRTLNPIDEELMYESVKRHGKCIVLTEEQVL
                     NSFAESLAGRISKNCFQYLDAPVEVVGSVNMPAVPLNSGLESMMLPNKDKVSKVIGEL
                     LGY"
     gene            complement(23964..26537)
                     /locus_tag="PLX80_01085"
     CDS             complement(23964..26537)
                     /locus_tag="PLX80_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19417.1"
                     /translation="MKISRFIIIFFSVAVFAVYLNSCSYLTYTTVTKNSNTDSTSSEP
                     DPDYDVSLNFDYQDFTSYLYMGNRIENFTAYFNTYFKAQQDYDEAFDEFRLSFIAIFN
                     RSIDSLLVNPPASQNVKDKLNKSIERSSKIIQFHKNSKYIDDAVLLIGKSYYFLSDYI
                     NAERKFNEFLSKFSSSELTDEAILYLARSKNKLNKENEAENIYLSLLKNETEGEIKSL
                     AARDYGVLEYKKGRPEEAAKYFRKAIEYTNDNVRKAESQFILAKLVSLFKPELAANEY
                     KIVLDYTSDFDLSFYGRLNYAKGLTYNKKFSLADEELTSLRKKYRDEIGYAQLVDLEL
                     ANNLYAQNKLKEAMDKYYEVIVKYPNSVASADAYFFIGRHEEEIENDYLNALVNYKKS
                     VQENASSDYYILVSEKVSDLDRYFTLQSEVTDSQPIDIPQENFEVEKYRAFYNEEKGI
                     EQFNETNKGGNNNEGNNKSGDENDGDQRGDGKGGPGGFNNYYFENLRDSTEDPIKEGK
                     WDKEQSDDQKNSGSDNSPNKNENNENNFNPGNQDKTEKNDTESKNDTSVVNTDSLKQV
                     QEEIDLKTKIEKEFNGYYELAELFTYKLDRSDSAEFYLKLILQKFKEPDQQSKVLYTL
                     GNFYKSTGKDVKAQETFSKLIGDYSNTIYAIEAKKIIGEVITEDDVVTSTADIDFKKA
                     LELYNDQKYTEAVDLLKSIRSNAKLDSIKAKALYGIGFVFENGSLNKDSSLYYYGVLR
                     SEYPESIYTQKISELLDYFAALEENNANDSLNTNSGNDSLKDASDTNNVAPTEINNED
                     EKKEEVVNPENENKENTDVNPEETGTDVGKDQLTPEEIQEMLKEAEKEGADPPPPPPD
                     N"
     gene            26830..28263
                     /locus_tag="PLX80_01090"
     CDS             26830..28263
                     /locus_tag="PLX80_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19418.1"
                     /translation="MKDTKLFTLLRSISKEEFTEFHDFIISPIINKDENFIILYDYIK
                     KNYSLALKDEIGKEKIYKAVFGKSDFNGSKYWKLTSGFSKLIDKYLLFTAFENDPFYH
                     KNLLLDIYRTRNVHKQFETLSKEIHRSFTKEFNKGLSYYLNRTHFYFQKLSYLGPENI
                     EEFEDDLKKMFENLKMFFIMTNITSVSIISNFKEKFPVDAKKDVWMFKEILAYLGKNK
                     QTVKNEDQMVYIFYLIILMKMDYSDVQNYFEIKKIITTSKLKFSKNLLRHILINLFDY
                     AVKKFTTGEEKFLKEIYFINKIMDENSVTLFGEFIQGEYFYSVVEHSVMLNEIEWTEE
                     FIRKYGKYLTDDYRDSALNLSRARINFELSDFDSSLNDLLNVENLNPYFYLSHKILLL
                     QNYFEIKDFESIDRLLETTQKYLNRRIDLSDELKKNYLKFFDYFRKLKTAATVKRYLS
                     RNLYKELEKESFFIYNNWLMIKTKELI"
     gene            28397..29236
                     /locus_tag="PLX80_01095"
     CDS             28397..29236
                     /locus_tag="PLX80_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+-dependent transporter"
                     /protein_id="HQY19419.1"
                     /translation="MDSLVILSLKLAVGLIMFSLGLNSTMSDAAYLLHNPKLLIKSII
                     SMNLIMPFVAVTMILTLGLKQEVNLALFALAISPIPPLLPNKALKAGGKISYTVSLLF
                     IMALLSIIFIPLSVELMGIANLKDFYVSPLVVLKVIGVTLIGPLLFGLIINKVALLFA
                     QKIKKVVSITAMILLIAGIIPLLIKLMPIVITLFGNLTILSLLIFIISGLVVGHLLGG
                     PVEDDRTVLALTTSTRHPGIAIVICMTVMPEVKLAVSVILLYLIINLIVSIPYIKWRK
                     KTS"
     gene            complement(29328..30650)
                     /locus_tag="PLX80_01100"
     CDS             complement(29328..30650)
                     /locus_tag="PLX80_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19420.1"
                     /translation="MKTKNFLSAVLSLLLFVLIFTFESKAQEIPNESGDNNQIFVDTI
                     ETSENLLDSNEVYKQQFEELSEDGDWIKVKKSDFIKELTEETGENLQYYYSDNGEYIY
                     VWQPYVADNNWNPYIGGRWVFSYYGWTWVSDYAWGWGPYNYGRWYSSSYYGWVWLPGN
                     VWAANWVSWRDCGGYYAWYPTCPSIYWYSYGNGYCGNTLYSYSPHNWVVVKYSDVTKR
                     IDEKVVGDPVDNVNILNNSTKMDISTYTDKETKDFKYTGPDVKQVTKESGEVITPKYV
                     SINTTKSGKYDEGVNYTSTKNSVTKSELNNPVTTKNENVKSTNDPNTKSTNTKYPNTK
                     STNTTKSPNTKSNNTTKSPNTKSTNIKKFSPPKNNGTKTESPEKIQKPGNNGKKENNG
                     TKNSGNIKNNNTKKDNGQNKVKSNSSNTNSNKGKSNNRSGKSNSRKRN"
     gene            31124..31501
                     /locus_tag="PLX80_01105"
     CDS             31124..31501
                     /locus_tag="PLX80_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HQY19421.1"
                     /translation="MNKKRFYYLGLIVFIIILGIASRKYSTLLPDFVSDYSGDTLWAL
                     MVFFIFRFIFPKHTIFYTAIISLIFSFVIEFSQFYQAEWLNNIRNLKYAGLLFGYGFL
                     RSDLVCYTAGILIGFLFEKYFKN"
     gene            31513..31896
                     /locus_tag="PLX80_01110"
     CDS             31513..31896
                     /locus_tag="PLX80_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19422.1"
                     /translation="MINTKNEFTEEMYQYGKSLIECLDKEGFIYPLVMWIKFPDKEEW
                     SLLFGIPGLKIIGSPDILNNMKRIINKNELNISAENISLIDSMDELFINIKSCIRLSG
                     ISRMKVSSTQMNNMILPESVYYRVS"
     gene            32056..34530
                     /gene="lon"
                     /locus_tag="PLX80_01115"
     CDS             32056..34530
                     /gene="lon"
                     /locus_tag="PLX80_01115"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQY19423.1"
                     /translation="MAKSKEEKIDFTEVDESIDENSSEVIYQNSSNIPDLNVAIPKDL
                     PVLPLKDMVVFPYMMFPILAGRESTIAAINSSLEKDKFIMLVSQLDEKIEEPTFENLN
                     KTGTVAKIIQVLRLPNGLIKVLVDGLVAAKVKKFKNDKYISAEVEVLNVQFKKDTELE
                     ALIRQATKLFTEYVNTNRTIPQETIVAFENIKEPDRKLFYIASTINADVAKKQRILEL
                     TDIHKQFYEILYILGSEMEVLNVEKEIDAKIYQTMQKNQRKFIVQEQIRILQDELGEG
                     METDPDLIKIREQIETSGMPEPILKKAMDEFNKLKKIPSMSPDHSVIRNFLDWMVSVP
                     WNNFTEDNFDLLHAKEILDEDHYGLEKAKDRIIELMAVLKLLDDKDIKKSPKGQILCF
                     VGPPGVGKTSLGKSIARAINRNFVRISVGGVRDEAEIRGHRRTYIGSMPGKIIQAMKK
                     AGSVNPVILIDEIDKMSSDFRGDPSSAMLEVLDPEQNSTFNDHYLDVDYDLSNVLFIT
                     TANVYYNIPLPLLDRMEIIEMRSYLDFEKLQIAKKHIIPKEIIEHGLTESMIKIPDPI
                     ILLVIQEYTREAGVRNLERELSSVIRKVTREIVEKGNPKKSKTIVITEEMVRKFLGVQ
                     KYKQKLADDKKSEKIGSIVGLAWTSMGGEILHVDVNIMKGANKFILTGKLGDIMKESA
                     QAGFSYIKSNASDFKIPESFFKEKEIHIHLPEGAIPKDGPSAGITMIMAMLSAITKNP
                     VRSDVAMTGEITLRGNVLPIGGLNEKLLAAVRSGIKTVLIPADNEKDLVEVPEEILSK
                     LKIIPVEKVEDAISHVFKNKNVIFKK"
     gene            34565..36064
                     /gene="dnaX"
                     /locus_tag="PLX80_01120"
     CDS             34565..36064
                     /gene="dnaX"
                     /locus_tag="PLX80_01120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQY19424.1"
                     /translation="MSDYKVSARKYRPQKFSEVTSQEFVTQTMKNAILSEKIAHSYLL
                     SGPRGVGKTTIARIFAKAMNCTDLKDGEPCNVCNSCVEITNGNHPDVFELDAASNRGI
                     DDVRAVQDAAKFFPIKGKYKFFIVDEVHMLTTQAFNALLKILEEPPEYLIFILATTNP
                     EKIPATIVSRCQRFPLQRIKINEISDKIKEIAKEEKVKIDDESVFLIAKLGDGALRDA
                     EGVFDMAVSFCGDNIIYRELKSFLNIPDKDIYFDMSEFIKNGDVKSILIYFNDLEEKG
                     FDMQTFFNGITEHIRDLLIVKSTSNPELLDESDSFREKYSVASKDFSTDQLHRLLKIL
                     FESESRFRYSANQKILLEIILAELCTVNRDVQDLSELISEVSSLKKKSLTEDLSEITS
                     EDIPKKDLYKTDNTESENQIKNEIETDKKTDQEADKNSEYISEQNTVTETKNKKQSEE
                     GSSEINKIENSEVHVNENFDKSHSSKSEKDSELVEKLKELFDVEEYKIN"
     gene            36066..36968
                     /gene="speB"
                     /locus_tag="PLX80_01125"
     CDS             36066..36968
                     /gene="speB"
                     /locus_tag="PLX80_01125"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQY19425.1"
                     /translation="MEKRIKFYGEKKNFLAIEEKYSDYKNSGIVILQSPYELTTSYGK
                     GTAEGPKSIIKASHYVEFFDEELDRELCFEKGNGICTLHPLDFKDKKSREALDYIYEN
                     VTKHIENGKFVVLLGGEHSISTAPIRAHFDKYENMSVLHFDAHSDLREEYEGSKFSHA
                     SFAARVAEFTKDITQVGIRAQCKAEYDFIKENGIKTFYAHEIRNGTYGDDWQSKVIEK
                     LKENVYITFDVDYFDPSIMPSTGTPEPNGFLWNETMKLLKMLGEKKNIVGFDVVELAP
                     VKAFSYPDFMTAKLVYKMLNYFRK"
     gene            complement(37061..38689)
                     /locus_tag="PLX80_01130"
     CDS             complement(37061..38689)
                     /locus_tag="PLX80_01130"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY19426.1"
                     /translation="MKSLFLLFIVILIFTQENANSQISQQWVQRYNGSGNGIDEAYSS
                     AIDIDGNVYVTGKSFLPASNYDFATVKYNSGGVQLWAKRYDNVGGIDEANSIVLDNSG
                     NVYVTGKSQGGASNSDFVTIKYNSNGDQLWLRSYNGLGNGIDNPVIINIDNNGNVYVT
                     GISWGGSSYDIATLKYNSNGVNEYAVRYNGTGNGDDFPVSMHTDNNGLVYVTGGSEGT
                     GSGYDVITIMYLQNGNANWIRRYNGPDNSYDFGRSITSDNNGNIIVTGESNFNNSGYD
                     YNTIKYNQNGDLLWASGYNGTGNGDDLGVSVKTDDQNNIYVTGSSNGVGTNEDYVTLK
                     YNSAGTQQWLQRLNGSGNGRDVPKSMVIGIDNSLYITGTSLNSGNFDYASVKYDTSGV
                     EKWVRTYNGNGSGLDAATSIAINNQEFVVVTGLSYGGSGTSYDYASVKYSQSTGIQNI
                     SNEIPGEFMLHQNYPNPFNPTTNIKFDILKAGNVKMTITDISGRVISEPINSKMTPGS
                     YVMDFNANELSSGIYFYTLLSNDFRITKKMTVLK"
     gene            38890..39324
                     /gene="secG"
                     /locus_tag="PLX80_01135"
     CDS             38890..39324
                     /gene="secG"
                     /locus_tag="PLX80_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQY19427.1"
                     /translation="MVTITILFLIVISIVLMAVVLMQSSKGNGLSGPIGGSSVTMAFG
                     VRRTSDILSKTTSALAVILMVGCIVVNLMIGGSGAVDESIIQKNAGQQQQQLPPNTIP
                     PDQNAPPQGETPDQTPDQTPGQNQETDKNAERPPVNVEPEGP"
     gene            39371..39946
                     /locus_tag="PLX80_01140"
     CDS             39371..39946
                     /locus_tag="PLX80_01140"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HQY19428.1"
                     /translation="MKIFHSAIFVLFGVIFLFINRADAQIKTDKISFYDLKEKVISKN
                     DTLKSNQETKQLFIMKKQPWRAVAYSAILPGAGQFYSESYWKVPIILGLGGYFVYGII
                     NNNNQYIDYRDQYQNSQTTENPNGNQQLLNLREFYRDQRDNFYIYSAILYLANLIDAY
                     VDAQLYDFNVSDKINFGLLKKNRLIGIEYNF"
     gene            complement(39943..42891)
                     /locus_tag="PLX80_01145"
     CDS             complement(39943..42891)
                     /locus_tag="PLX80_01145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQY19429.1"
                     /translation="MSKFKTTLAMINIFIAVILIFVTSSLYSQELKKVTDGKYTYEEV
                     ENDPLKARIYTLDNGLKVFLSVNKVEPKIQTYIVVGSGSKNDPPTSQGLSHYLEHMLF
                     KGTDKFGTKNFEAEKPLIDSIIALYEQRRLATDPDERKLIYKKIDSISYVASEYAIAN
                     EYDKMIADIGGDGSNAWTSLEETVYTCNIPTNQLERWLTLEAERFRNPVMRLFHTELE
                     AVYEEKNTTLDDDNDKVWQAIMEGLFPSHTYGTQTTIGSVEQLQNPSVQAIIDFYNEN
                     YVTNNMAICLSGDLDPEKTIELINNTFGKFESKPDPEFVSPVQDPINGPVIKEVVGPS
                     EESVMFGYRNMGADTKDADILTMMEYILSNGSAGLIDLNLNQDQKVIDAYCGTYNNKD
                     YSLMYLGGKPREGQSLDQVKDLLLQQLELVKKGEFPEWLIEATINNFKKNQLQSYEKN
                     SSRGYSFLKSFILDIPWNKYIFNVDRLSKITKDDIVKFANENLNTNYVIVYKRTGEDK
                     SIVKVDKPEINPVKINRDDKSDFIKEISNMSYEEIQPEFADYDKDITKLSLNNNIPLF
                     YVENKENDLFYLNFISEVGTNQNKKIALATAFFDYIATSKYSSSELKEKFYRLASSYS
                     VSVSEDQLSVSLSGLNENFDESLKLLEEIISDPKVSSEALTNLKSDINKEREDAKLDK
                     STILWSGLYNYGKFGKDNPFTYRLSKEELNSVTGEEITEIIKDMFSFKQRAYYYGPLG
                     NSEIISSLNEDHKTPSELKTLKTLNKFKEEETEENIVYVVNYDMQQAEIVFISKKRPF
                     DLGIVPIASMYNEYFGGGMGSVVYQEIRESKALAYSTFSVFQMPKKNNYSHYNIAYIG
                     TQADKLPEAMAGMTDLLENFPASEVAFNNAKSTLIKQIQSERTSPKGLLGEYENNLQL
                     GITENIDKKIYENIDKFTLNDIVTFQKQNVKDSKYTILVLADTKKIDMKVLEKYGKVK
                     VLTIKDVFGY"
     gene            43089..44078
                     /locus_tag="PLX80_01150"
     CDS             43089..44078
                     /locus_tag="PLX80_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19430.1"
                     /translation="MKNQNFYKLKYPLLIALFLTVIISSCSDNTIENGALQTDDEYIR
                     NIAMNSSFSSNEDDDDNLFYSDEMDFESEGATYSNANNNTPLDSVLKWGRRITGKNVN
                     VNITTHGDTLKDVIVTKAISGNFIIIGYTGGILDSAVKPFSQEHKRLISFKRVDRRQH
                     PRHNWRVFQYSAVDGETKTPQIGKENITMNRVEYYRNNELILTLNGPDFTTNIFSARH
                     FGNQSQIELVRGDQVKIKVFLSSNQIDEDIVAYHWGRNSHGFHRELFEMTTEIQNGNN
                     FDRTFEKTFEIYQNHKHGMHNGFISSITRSSLFDNSTSLFSSTYMGLPYRVRH"
     gene            complement(44315..44704)
                     /locus_tag="PLX80_01155"
     CDS             complement(44315..44704)
                     /locus_tag="PLX80_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19431.1"
                     /translation="MDKAKLTGTSSILLVSDIDKSAEWYREKLGFDFQNFYGDPHEFC
                     VMNRDNIYLMISQSHKTEDIKPNWKSKDKTSNVYFWVNDAEKIYREFIVRGAKIDYSL
                     YVTDYNIKEFGVSDPDGYDISFGEKLI"
     gene            complement(44691..45149)
                     /locus_tag="PLX80_01160"
     CDS             complement(44691..45149)
                     /locus_tag="PLX80_01160"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HQY19432.1"
                     /translation="MDNNITINDNMPSNEDRTMALLSHLSLILGGILLPLIIWATQKD
                     KSKFVRFHSLQSIFFHLSIAAVIIFFVIFFAVIFFASGLGMSNLNSSGNSELPAFFII
                     LIIAFYAFIILLVLGAIAYSIYLALKAYKGERIKVFFIGNIIYKKVYGQS"
     gene            complement(45174..>45433)
                     /locus_tag="PLX80_01165"
     CDS             complement(45174..>45433)
                     /locus_tag="PLX80_01165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQY19433.1"
                     /translation="AFVIKDKFPQSKGHLLVIPFDHYENYFDLPEDVRNSLWDLMEDA
                     KKLSDKNHSPSAYNLIINNGKDAGQIVMHSHIHLIPRYSKK"
BASE COUNT        14599 a         7778 c         7529 g        15527 t
ORIGIN      
        1 acaatataaa attacccaac tccgccaggt tcggaagaaa gcaacggtaa gtaaccttta
       61 tatgtgattt gggtgtcttg acaatttttt ttatacttca aatccaatta cctaaaaact
      121 aatatgagta aattaaaaat cggaattgta ggactcggcg ggatagcaca ggtaattcac
      181 ctgcctatat tgtcaaaaat ggctgatgta gagatcagcg cagtctgcga cagtaatgta
      241 tcaaaatgta aaagcatatc ttcaaaatat agtgtaaata aatatttcaa agatgtagaa
      301 aaaatgctgg aagagtgtga agacatttct gctgtaatag tagctactca gacaagtgtt
      361 cacaaagaca ttgcaaaaaa atgtctggaa gctggtaaag atattctgat tgaaaaacct
      421 attgcacgaa attataagga agcaaaaact atagttgata ttgcggcaaa aatgaaaaag
      481 aagattatgg tcgggatgaa taacagattc aggaatgata tgatgcttca acggactttc
      541 acaaaagcag gtgagatcgg tgagatcttt tatgtaaaga caggatggat aaagccgaaa
      601 agttcagatc agaactggat gcttcataaa gtactttcgg gcggcggtgt ttttcttgat
      661 accggaattg caatgctcga ccttggtttg tggatacttg gttttccgga aatcaaaaaa
      721 gtatcagcaa caaattatta tcacacgaca aaatctgtag aagactccag tattgtcata
      781 gttagtttta aaaataaagc aacacttaca gtggaagtta gctggggcat tcaaagggaa
      841 ggagaacttt tttattgcaa tgcttacgga aagtctggca gctcttccat taatccgttt
      901 agaatatata aaaagatgga agataatttg tataatatta ctccaaaaaa attacctctg
      961 ccttctaatc agtttaagaa gagttatgag tatgagctgc agaattttat aggtgctgtg
     1021 catggtaaaa atgatctgat ctcaagcgga aaggatgcac tgaaagtaat gcagattgct
     1081 gatgctgttt ataaatcagc aaagtccgga aaggaaataa tttttaaatg atttgtttaa
     1141 gattaattaa cataggttga ctatttttat gaaaactaat caaatgaaat taaaaatact
     1201 tgtagtagac gatgaaaaag atattgtaga tatacttaaa tacaatctcg aacgagaaaa
     1261 tgaatttgaa gtgatccccg cttataatgg aaaagatgct ttagaacttg cggcaaatat
     1321 tcctgatctg ataatacttg atattatgat gcctgaactt aacggctatg aagtgtgcaa
     1381 aaaattaaag agcaatcctg aaacttccgg aatacctgta atttttttga ctgcaaaaga
     1441 aaatgagatc gatgagatca taggtctgga aattggtgct gatgattaca tttcaaaacc
     1501 catttctcca agaaaagtaa ttgcccgaat aaaatcagtt atccgaagaa cttctatcga
     1561 ttcagaaaaa ttattcaaat ccgatcaaaa gataactttt caaaatctgg aaatagacag
     1621 tcagagtcac agtgtaaaaa tagatcagaa ggatatcttc ttccctaaaa aagaatttca
     1681 acttctccat tttctaatct caaacagagg aagggtttac tcaagagaaa ttcttttaaa
     1741 tcagctttgg ggtgaaaata tttatgtagt cgatagaact gtcgatgtgc acgtagcaaa
     1801 ggtccgtgaa aagcttggtg aatattctga ttatattgaa acaattaaag gacttggata
     1861 cagattcaaa gataattaat ttccgcactt tattttattc atgaaaaaag taaatctcct
     1921 tacgaaatat tctttgatat acttctcttc actgataatt ctcctgatcc ttttttccaa
     1981 tctgcttaac ctcaaaacag atgtaatctt tatttccgga atattcgggc tcataatgtt
     2041 ttttgtcagt atgatctttt tttactttga aatatccaga cctttgaaaa atatcctcag
     2101 catttctgag aaactaaagt caggaaaaga ttctgataag tttaataatt ttgaatttaa
     2161 gatcactgat ctgaaagaga tcctgaacaa aataagtgaa gtaagaaata atacaacagg
     2221 aaacagagag gttcatcagc ttataaatga aatacatctc atcgctgaaa gaattctgaa
     2281 agaacttgag cttgcaaaag tatttaaagt aaatcgaaat gaatttatgg gaaatgtagc
     2341 acatgaatta cggacaccga tctttgcaat acagttgtct ctcgagacat tgctggacgg
     2401 tgcaatcaat gacgaaaaag taaacataga ttttctgaac agagcattca atcagacaaa
     2461 acgtctaaag gagctggtcg aagatcttat cagtattgct aagtttgaaa caggtcttaa
     2521 gatgagtaaa agatattttg gaatttcgaa tgttatttcc aaaacacttg aagagcttga
     2581 agatatagcc gaaaccaaga agatccagtt gaaatttgat gctgatcctt tgaatggtat
     2641 tcaggtcttc ggagacgagg aaagacttaa acaagtgttg atcaatctga tagataatgc
     2701 aattaaatat actccggaag gcggaataat aaatattgga gtggatgtac atgaaaagga
     2761 tgtgattgtg aaagttcagg acagcggaat cggaattccc gaaaaggatc taccgaggat
     2821 ttttgaaaga ttttacagag tagataaaac aagatcaaga gatgtaggcg gaagcggtct
     2881 gggtttgtct attgtaaaac atattcttga agctcattca agccgcatta ctgtagagag
     2941 tattaaaaat gaaggaacga aattcgagtt caatctgaaa aggtaataat aatattaagt
     3001 tataagttca ttccagatct tcagtaaacc tgtttcatca aatttctttg acattacctg
     3061 tattccgaaa ggaaggttat tcctgtcttt gccgcatgga atcgagatcg ccggtatacc
     3121 tgcaagattt acagatgcag tataaatatc attgagatac attgaaagag ggtcttcaat
     3181 tttttcacca attttaaaag cagtattcgg agtagttggt gaaattataa aatcaacttt
     3241 ttgaaatgca ttttcaaaat cattttggat aagtcttctt actttctggg cttttctgta
     3301 ataagcatca tagtatccgg cggacagaac atatgtaccc agcattatcc gtctctttac
     3361 ctcttcacca aatccttcgg atctagtatt cacatacata tcttccattg tctctgaatt
     3421 ttctgatcta tagccgtatc tgacaccgtc aaaccttgac agattactgg atgcttcggc
     3481 agaagttaag atataatagg tctgtattac atattttgta tgaggtaatg agatctcatt
     3541 tacaataaat cctttttccc taagtaaatt tattttatca agaacaccct ttttaatatc
     3601 tgggtcaagt ccttcctcaa aatattctgc agcataaccg atctttattt ctttcgggtc
     3661 aaatgttttt tccgataatt cagaatatgc aggaacagat acttctgatg aagtactgtc
     3721 tctgtcatct tctccggcaa tgacttcaag gagtattgca atatcgttat tattatttcc
     3781 aaaaattcca atagaatcaa atgatgaagc aaatgcaaca agtccgaatc ttgaaattct
     3841 gccgtaagta gctttcagac ctgtgatacc gcagaaagat gcgggctgtc tgatagatcc
     3901 gccggtttct gatccaagtg aaagcagaca catatttgca gatacagatg cagcactgcc
     3961 gcctgaagaa ccgcccggta ctcttgaagt atcaacagga ttttttaccg gtccgaaata
     4021 ggaattctca ttcgaagatc ccatcgcaaa ttcatcacag tttactttcc cgattataat
     4081 tgcatcttca gataatagtc tttctataac tgtagcatta tatacagttt caaattcact
     4141 taacattttt gaaccgcatg tcaacttttt atcttttacc gagataacat cttttacaga
     4201 aatgactgca cctgccagct taccggcttt gccttctttg atcttttcat cgatctctcc
     4261 agctcttagc aaagcttcat cttcaaacaa agaaataaag acattcaggt ctttctgatc
     4321 attaatattt ttaagataaa tttgtacaag ttctgcacag gtaatattac cttcagtgag
     4381 acctttcctg atctcactta aatttcttgc cggactattc ataaaatttt gttattaata
     4441 tcagatatga taattgaaga ctattttgaa aatattctga tttttagaat ttaactattt
     4501 attagaagtc agttggaatt ttttttatct ttgtcatctg tagaggtcag atctttttca
     4561 atatcagaag atgctttttt gaattcttta attcctttac caagtccctg cataagatcg
     4621 gggatctttt ttgctccaaa gagaactaaa ataacaattg ctataagaat gatttcgggt
     4681 gtacctaatc cgaacatttt gattactcct ttccgcttag cggattaata aaaattataa
     4741 taaactttct gcgacgcttt taaaaaatct gtatccgtct gtgtttccaa tcgcttcatc
     4801 agaagctctt tcagggtgcg gcatgagtcc gagtacattc ctttttacat tttgaattcc
     4861 tgcaatatca tcaagtgaac cgtttggatt atctttatat ctaaagatga tctgattatt
     4921 tccggttatg ctgataaatg tttcctcgtc acaataatat tttccttcgc cgtgagcaat
     4981 tggaatttcg atctcttcct tgatcctgta ttcgcctgta aaaatactct tattattttc
     5041 tactttcaat gtaacagttt tacagacaaa tttcagattt tcatttctta ataaagcacc
     5101 cggcagaagt cccgtttcac aaagcacctg aaatccattg cagattccaa tcacaacacc
     5161 accgttattt gcaaatttta taacttcatt cataagagga gaaaattttg caatagcacc
     5221 gcatctcaga taatctccat atgaaaatcc gcccggcagt ataataatat ttctgtcgcc
     5281 aatatccgtt tctttatgcc atagaaattt tgtatccgca tttaatatat gtctgaatac
     5341 attatagaca tcatgatcgc agttagaacc cgggaatact attactccgg cttttacgtg
     5401 atccatttat ttaccttccc cgtctttagt aatgctgaat tcataatctt ccattacagg
     5461 atttgcaagc aattttctgc aggtgtcatc agtaattttt tttgcttctt cttctgtagc
     5521 tgcatcgatt tttaattcta tatattttcc tatccgcgta tcaactacag aatcaaatcc
     5581 aagtgacttc agtgaatgct caactgcttt tccctgcgga tcgagaatag tgtccctgat
     5641 cctgatattg atcttagatg taaacaatct attgtgattt attttataaa attattttat
     5701 gatttcattt cggatatttt ctaaaccggc tttgtttatt ttatccaact ttgtagacag
     5761 accattatct ccactttcat ccgctgatat agattcttta ctccttttta ttacaatttt
     5821 ttctgaatca gacttagaac ggctttttgg ggttgccatt tcatttcctt tagctacttc
     5881 tgtttttaca tcagcagtta cattagtctc gctttctttt tttaattcag gtatagtttt
     5941 attttctgtg ggtttgtcag tttcattttc agttttagtc tctgaaggag aaattctttt
     6001 tttattatta tctgaaaatg ccagatcaga gttttgagtt aaaccatttt tcttttcatt
     6061 attcatattt tcatttgaac tttctccgtt tttttcttcc tcaacttttt ctttatcctg
     6121 ttcaattaat tccgcctgta atttttcctg ctctgtattt tttttcagct ctgcagtatt
     6181 atctttattt aaagtttctt gtttaatatc tgcagaaatt tcattttcat tattagtaaa
     6241 attatccctg ctgataaaag taatatagaa tacaaagaaa ataaccactg caaaacctac
     6301 tgatggaata agccatggat actgtctgtt attaaaaaga tttctaagaa agcctccttt
     6361 ttcggcatca aattttttga tgtgttcttt tcttctggaa gattcctctt cgactattct
     6421 gagatgaagt ttgtttgtaa agtcgctgct cgcatgaact gtctccagac ttctcagctt
     6481 ttctctgatc tcaagatatt tttcttcgtc attcaaaact aattgtaaat attttttatt
     6541 ttctaatttt tggtttatgt aaactttcta aaatttcttt caaacattcc ctgccgcgat
     6601 tgatcctcga ttttactgtt ccaagtggca gagatgttac tttggctatt tcttcataat
     6661 caagatcctg aatgtcacga agtacaatta cttcacggaa tttttcattc agagaattta
     6721 tcgctctctg aattatctcg ccttcagttg cagcattagc actatcatct ggatgaatat
     6781 aagttgatgt aagatcgtaa tccttttcct catcttcata ataattactg atcgacttct
     6841 ttttcatctt tcccttttta ctcatgtaag tcctggtaag gttcagtgcg atagtataaa
     6901 accatgtaga aaattttgcg atctcgacat ataaatgttt tgacctgtat aatctcagaa
     6961 atgtatcctg cgcaagatct tccgaatctt ctctgctacc ggtatatctg aaaataaaat
     7021 taaccagtct gtctttatac ctgtttacca gttcattaaa agcttttaca tcctcacttt
     7081 gaaacaggag tatcagctct tcatcggtta gtattttata ttctgttttg gattttccgg
     7141 gcattaaacc taaattaata aacttctgta aaaactattt gttccggcat atttcataat
     7201 aaataatcat aattgatttt tcatttttta tcaaaattac tgtcatcgaa ttttgcaaaa
     7261 ccattgatcc taattcattt ttagtcagca catatattat tgatcaatga aaacattttt
     7321 gtaaacttta ctccgccggt tgattttaac aaaatatcag tattatacag attttcaatt
     7381 atattcctaa tctttttaga tgaaatagat gaagccgttt tttggtaaag aggcagtaat
     7441 gcaagctgtt cctctgacca taacttaagt tctcttttga gattaaattc attcagtctt
     7501 gatgatgatg gatcctgtaa tttaaatatt gccataaatg ctgaactcaa taaaatcacc
     7561 agatatactt ctacttcctt tttaagagaa atttttttat aaattttaat tgcaagttca
     7621 ctatccttcg aaattactgc tttgatgaaa tcagtttcct taaaatcctt tgcaattccg
     7681 ttgcataaat ttactgaatc atttgaaacc tctttagaag agtagcaata agtcttaagc
     7741 ttttcgattt cagaaagcag ttcaccggga gaataatttg aaaataacag taaatgacga
     7801 acggtgtctt tagaaatttt atatccatcg aattttttaa caacccagtc tattaattca
     7861 tcttcgctga attcttccgg cttaaataaa tttatcctgt cactaaaatt ctgaatacct
     7921 tctccattgg tctttgacaa aaataattca gcatcaaaga tcttattgat ctcagttgag
     7981 atatccggct caataagtat cagtaaagta tctggatttg atctttttat ataatccgct
     8041 aatgcaattt tatcattctt taaaaatttc cttacattcc taactacgac aacctttctt
     8101 ccgccaaaga gaccagtatt gctgcattca tttaaaatac tttctgttac tttatcatca
     8161 gcattaaaat gcaacagatt attctttctg tcaggatcat tgtttgtaaa tgcattataa
     8221 aaattatcta tgatctcatc tgcaattatc ttatccggca tataaaaaaa gatattattt
     8281 ttatctatat aatcagccgc attttcacaa agcttattta ttgaaatgct tttagctgaa
     8341 aggatatttg aagaattttt tgccataggc attaaaatag tcaatatgat taaattaaag
     8401 aatattaaaa tttttatgtg tagaaaagag atgttttata gatagttgat aatttatcaa
     8461 gtccgggagc cttatataat atagttccaa tttatattac aaatataaat tttaaaaatt
     8521 tgtatatttg aaataataaa aattgatact aacagttcat taccaaaaat tattttgatt
     8581 attaaattgt gttttaaaac agtaaagttt tgttttttta tagtaataat tttttatgtt
     8641 attgaatcag gtgcggaagc ccaaatacta aagtggcgtg atcttccgaa ttcaccatac
     8701 aatccgggcg gcagatttga tgatgtttat tttataaatc ctgatacagg gtggataata
     8761 agtatcagtg atgtagtgag gactaatgac ggaggaaaca attgggatac aacattcaga
     8821 attccgggtg gtaatttcag atccattgga ttttttgatc ctcaaaatgg tcttttggga
     8881 actttgagtg aagactcgaa taaagtttta tacagaacta caaacggggg agtaaattat
     8941 acagctataa ataatttacc accacccggt ttaaaaggaa cctgcggaat aaatattgta
     9001 gatgaaaata ctgcatacat ttgcggacga tggtttacac ctgccagaat tttaaaaacc
     9061 actgataaag gaatatcatg gacttcattc actatcgata cagcacttgc gaattccttt
     9121 gtagattgct atttctggtc tgcagattcg ggaattgtag ttggcggata cagaacaata
     9181 aacttctctg caggtaaatc agctatattg atgacgacca attcaggagt aagctggcag
     9241 cgtgtttatg tatcttcacg tgacggagag tattgctgga aaataagctt cataaataaa
     9301 aacacaggtt atgtaagtac agaaagatca ggattcacaa attatttaaa aactaccaac
     9361 ggtggattca actgggaaga aaaaccattt ttaaataact atacagttca gggcattgga
     9421 tttttaaatg aaaccacagg atggatcggt ggatttggag gagcaaatta tgagactact
     9481 gactcaggaa attcctggca tattgccggc tggggcagac aaatgaacag ggtcagattt
     9541 ttgaatgatt cgttaggtta tgcagtcgga caaagaactt ataagttttc aagtgaacca
     9601 gtcggaatta caaatatttc caatgaagta cctgataaat ttcaattgtt tcaaaattat
     9661 ccaaacccat ttaatcctgc cactataata aaatttgata ttgaaaatga acatgatcag
     9721 catgtcaact taattatatt tgatgcgctg ggaaaaagaa tatctacttt ggtaaatgaa
     9781 aaacttaatg caggcactta tgaagttgac ttcgatggaa ataattatcc aagcggatta
     9841 tacttttata agcttcaaac aaataaacat tctgaaacga aaaggatgat tcttttgaaa
     9901 tgattcttct tattaaattt gcctacaagt ctcaaaggtt taaagagtca tattttaatg
     9961 tttttaaact ttgagtctta gagccttcga ggtaattcca aagataataa aaaaagccgt
    10021 ctcaaaaaat ttgaaacggc tttttaaaaa atgaaaaata aaatattact tggcaactac
    10081 agtcgcttta gtaattttta cttctgttac aggcttatca ttcggacctg ttttagtttt
    10141 accgatcttt tctacaacat ccataccgcc tactacttca ccgaatattg catgttttcc
    10201 gtcaagccag tctgtcggag cgagagtaat aaagaactgt gaagaacctg tattcggtcc
    10261 tctgttagcc atagacagca ttcctttttt gctgtgcttc agtccgggtc cgaattcatc
    10321 tttgataatc tcttttgaac cgccggttcc tgttcctgta ggatcacctg cctgtaacat
    10381 gaatccgtct ataactctgt gaaaaattat tccgttataa aatccttcat tcacgagttt
    10441 tttaaaattt gcagtcgtca gcggagcttc ttttgtatag agcttaattt tgatgttacc
    10501 cattgtagtc tgcatgtcta caaaacttcc gtctgcgctt ttttcttctt ttcctgctgt
    10561 ttcattgttt tccattgtag agtccttttt ttctgaggtt tgagtttctg aattagtttt
    10621 tccggttgta tctgtttttg tttcagtggt ttttgtttct gtttttgtct cagttgtttt
    10681 cttttcttcg gttttagttt ctgtagaatc tttcttatca ccgcatgaca ttaataccaa
    10741 tgcaatgata actacagaaa aatataccgc aattttactt ttgtacatct atttctcctt
    10801 atttaaataa attaattaaa ttaatataat tttaaactaa aattgtttta aaaaccttat
    10861 gtcattttca taaaataatc tgatatcagg aacaccgtgt tttatcatca aagtcctttc
    10921 aattcccata ccaaatgcat accccgaata aatttcagga tcatatccga ccattttaaa
    10981 tacattcgga tcgaccattc cgcaaccaag tatctcaagc cagcccgtgt atttacaaac
    11041 tctgcagcct tttgctttac acagaaaaca ttctacatcc atctcggctg acggctcagt
    11101 aaacggaaag aaagacggtc tcaaccttgt cttcaaagat tcaccaaaat attctgtagc
    11161 aaaataattc agcgttcctt taagttcgct gaaagttaca ttcttatcta cataaagtcc
    11221 ttccacctga tgaaaaaacg ctaagcttct tgagcttaca tcttcatttc tgaaaactct
    11281 gccgggagaa ataattctca caggaggttt aatcttttcc atgatcctga tctgagctga
    11341 agatgtctgt gtccttaata aatatttctt gtctttacct gtgctgtcaa tataaaatgt
    11401 atcctgcata tcccttgaag gatgatgctc gggagtattc agcgcatcga aattatgata
    11461 ttcatcctca attgcaaatc cctcatatac tttaaatccg atcttttcaa aaatggctga
    11521 gatttcttca agtgcctgat ttattaaatg ccgtgtgcct ttgttgaatg tccttcccgg
    11581 aagagtaaga tctggtgtat ccggatctgc agtgttttca gaatccagtg cagacttttt
    11641 ttcatcaaaa attttctgaa gcgagatctt gagttcgttc agagttttac caacctgtcc
    11701 tttagattcc ttatctacat ttttaaaatc ttcgaacagc ctgctgagaa taccattccg
    11761 gctcagaaat ttaagacggt aatcctcaag agatttatct gagtttataa tctctgattc
    11821 tttgagcgct tctgatttaa gggattcgat cttatctaac attacaatct ttttgaaatc
    11881 taattacagt ctgattatta tttaagtgaa atttctttcg gaaatgaaac aaggcgaact
    11941 cacatccgcc ttattttcaa aatttaattc agtaataaaa tttattttaa ggcaaatttt
    12001 acaacttctg taaatgcctc agcattacca tatgcaaggt cagcaagcat ttttcggctt
    12061 atgtcaatgt tctttttgtt taaagcattg atcagctttg aatatgtagt gtcatttagt
    12121 cttgctgcag cattgatcct tgtgatccaa agacttctgt attctctctt ctttaacctt
    12181 ctgtcggtgt aagcatgagt gagtcctttt tctacggcat ttttggcagc agtatatact
    12241 ttactcctgg caccgtaata tcctttggcc tcttttaaaa ttctttttct tcttctgtgt
    12301 gatgctactt tatttcttga acgcggcatt ttttctcctg taaaaaatta ttattaaaaa
    12361 tgtttaagct tgtatcattc ttttaacagt tcttgtttcc tgctctgata ccaaagtagc
    12421 tttaccaagc tgtctttttc ttttgtttga tttttttgtg agaatatggc ttctgtaagc
    12481 tttctctctt tttacttttc ctgtgccggt aactttaaat ctctttttag cggctctgtt
    12541 tgatttcatt ttaggcatct tctttaccgt ccttattgtt atttacttct aaaatacttt
    12601 ctgaattatc agttgaatta tcttctgatt ctatattttt tgtttcttta tcttctgatt
    12661 ctatactttc tgattctata ttttttgttt ctttatcttc ttctttttta ctttcagttt
    12721 ttgccagttt cttttcaagt ttcttttctt ttgcatcagc atctttaata gcctgtattt
    12781 tcaacttgtc cggcaggaaa aaagcatata actgtttacc ttccattctc ggagaagctt
    12841 ccagttttcc aatgtcattc agcttctcta ctatttcatc cattaatttc cttccaatct
    12901 ccggatgagt gatcatccta cctttgaatg tgacataagc ttttacttta tgaccttcca
    12961 taaggaattg tctcaaatgc tttgtcttaa aatcaatatc atgtgtgtca gtatttggat
    13021 taaaacggat ctcctttata ttcatcacat gctgattttt cttcgccagt ttttcctttt
    13081 tctgtctttc gtaattgaac ttgccgtaat caacaatctt acaaaccggc ggttttgagg
    13141 cagatgagat ctctacaaga tccattcccc ttgatgaagc aattctcatc gcttcattgc
    13201 tggacattac tcccagtgca tttccttcat catctaacac ccttacctgc ggtgcgcgga
    13261 tctgatcgtt tactctctct ttgtactttt tatttttaat aaactatagt atttagttaa
    13321 ttaatctgat aaattaatta tacccggctt tttccaggac ttgcaagtta atcttttcaa
    13381 tgaaatcttt caattcaaat tttcctgtat cacctttttt atgctccctg acggaaattt
    13441 catttccttc tacttctttg tcacctatca caagcatata aggtattttc ctgttttcag
    13501 attctctgat cttatatccg actttctcat ttctcttatc tgtaaatact ctgaatcctt
    13561 tttctctaag cttttttgaa actgcatccg catattcgaa atgtgcatct gcaatcggta
    13621 acacactaac ctgaactggt gccagccata aagggaaata tccgccgtaa tgctctgtca
    13681 atactccgac aaatctttcg aatgatccga atattgccct gtgtatcatc accggtacat
    13741 gagtgtttcc gtcacttccg acatactcaa gtccaaatct tccgggcatt gaaaaatcca
    13801 gctgtatagt tccaagctgc cagtttcttc ttaggctgtc cttcactaca aaatctattt
    13861 tcggaccata aaaagctccg tcaccttcat ttatcttata ctttatttca gctttttcca
    13921 aagctgtagc aagcgctttt tcggaattat cccatacttc agttgaaccg atatattttt
    13981 caggacgtgt ggacaaatat acctgcacat catcaaatcc aaatgcatca tatatttcaa
    14041 atacaagctt tattagaatc gttacttcct cttctatctg atcaggtgtg cagaatacat
    14101 gcgcatcatc ctgtgtaaaa cttctcactc tgaaaagtcc gttaagcaca cctgataatt
    14161 catgtctgtg cacaagacca aattcatata atctcagcgg cagatccctg taagacttca
    14221 tgccagtttt atagacgagc gtactgcccg ggcagttcat cggctttact gcataatctc
    14281 tttcatcaat atcagtaaaa tacatattct ctttgtaatt gtcataatgc cctgactgct
    14341 gccataattt ctgatttaat attatcggtg tattaatttc ctgatagtca agttcagcaa
    14401 gttttgctct gagatactct ttaagattat tcaaaagcac catcccgtta ttatgccaga
    14461 atggaaaccc cggaccttct tcatgaaaag aaaaaaggtc aagctcttta ccgagttttc
    14521 tgtgatcgcg tttctttgcc tcttcaagat ttttcagata ttcatcaagt tccttttgtt
    14581 ttggaaatga aattccgtag atcctctgaa gcatctgtct tttctcatcg ccgcgccagt
    14641 atgaacccga cacagaaagc aattttaccg acttcacttt ttctgtcgaa ggaagatgcg
    14701 gtcctctgca aaggtctgta aattctccct gcgaatatag cgaaacctca tcttcatctt
    14761 tggcaatggt ttcaagtatt tctactttat aaggatcttc tctctttgac ttaaaaaatt
    14821 caattgcatc taatctctta atatcctttc tctcaggctt cagatctctt ttagaaattt
    14881 caagaatttt atcttcaatt ttctttaggt cttcttcagt gaattttctg tcagaatcta
    14941 tatcataata aaatccgctt tctataggcg gacccacacc gaatcttgca cccggaaata
    15001 attcctcaat cgcctgcgcc atcatgtgtg aagtagtatg ccagtaaact tctttcccct
    15061 tatccgaatc aaatttatga aagacaacct tcgcatcctc atttagaggc aatgaaatat
    15121 ccttcatctt tccgtttatc tccgctgcta aaatctcatc agcaagtctc tgactaatgg
    15181 atgcagcaat gtcataagct gaaatgcctt tatcaaactc ctttacagta ttatcgggaa
    15241 acgttatttt tattttatct gacatattta aattatattg ccattaacta ttaacttcaa
    15301 atctcagtat ttgtaatttg aaatttacaa tttgaaattg attcgtgggc ggtaatggat
    15361 tcgaaccaat gacctctacg atgtcaacgt agcgctctaa ccaactgagc taaccgccct
    15421 ctgaaatttg gaactgtcaa aataccaata atatgcggtt ttttcaatga gtgttttttg
    15481 cctttttgga atttgttaaa ttatgacaaa tcaattgggt tgcaaattcg ggtttttttt
    15541 tatgcgattt tcttaaattc ttttaattga attcacaaat attaatttca tattaatata
    15601 aatttaaggt tatgaaaaca tgaaaatttt tttaatatct gctttactgt ttttactcat
    15661 ctgtacttct caatccattt cacaaagtaa ttctccaaat ccaaatgcat gggcagttta
    15721 tagtggagcg gtaaggacag tcgctgcata tggagattat gtatatatgg gcggagattt
    15781 caaatatgtc ggacccaaaa ccggttgtct ggtaaaagtt tcagcgaata caatattagt
    15841 tgacaatgct tttccgaaag tggaagttaa tgtcgtaact gctgtagggg atgtaaacgg
    15901 cggatggtat gtcggaggaa attttaattc aataggtgga gtaacgagaa ataatattgc
    15961 aagaataaat tcagacggaa ctgtccatcc ctggaatccg aattccaatt tcattgtaaa
    16021 tcaaattgtt ttgagcggta atgatatttt tgtatcagga ggatttagct ttattggagg
    16081 tcagtcaaga actcatattg caaagcttaa caaaatacaa cgggtgccgc tgatatttta
    16141 tggaatttcg ccgttattgg tcttagtgag caaagcgagc gtgaccaaca acaaacggaa
    16201 actggttgtt ttcagaatta agtcttttga tttcatctga ctttattagt gaaaaatgtt
    16261 ttacttgata aaaattgaat atggttttta aaaaattata tagagatatg tttatcgcag
    16321 acttcgccgg cactgttgtt ggtgatcaaa tagtttgcac tagcggagca aaaaatttaa
    16381 cgaattatta ttgcaaactt tttgaaaaca ccaacaatga cggaattcaa tgaattcaaa
    16441 aataactaag ccagggactt ttgttttctg atttaagttt gccccaccat ttacacggga
    16501 aataaagcag taatattatc ccgatccaga caagataaac aacaccaagt gaataaccaa
    16561 atccgctagg cgtgttaaaa tttctgctga aaaaatccat gttggttatt gaatttcctg
    16621 agaataaata aaatatccag atcgcagaat gaataaaata aagatgaagc atgtaaaaga
    16681 aaaaaggaac tttcccgtat actatgatta ttttagagaa tttatttgaa atgttttctg
    16741 taaaagcgag aataaacata cagggaccga cggtcatcag taaatacagc agtgacggcg
    16801 gatacttggt ggtgttgaca aatgacaata ctgaatataa gaaattcttt tgtgtgtccc
    16861 agtttccggg atccccgtaa atattttccg cccggagaaa aatgaataaa agtatcgaaa
    16921 ctgttcccac tgacaataag atctttttcc tttttacttt atcaaatccg gaagcataaa
    16981 tatttccaaa agagtatccc gtgatcatca gccccagcca cggcagaaat gaataaagcg
    17041 caacgactct tcttcctgaa attataaatt cccttggctc atgaattatt gaccaaaaca
    17101 ttgaatttcc tgaatatcca tccagtatat tatgtccgaa aattattata agaccgacag
    17161 gcagcagaat ttttttgggc agatagatca atgctgacat taccaccatg gaaagtccta
    17221 tcacccatat taccccgagc agtaatcttg gcacgaacaa actaaatgac cacgcaaaac
    17281 ccactatcgt gaattcgcag atgataagcc agaatccccg cgttaaaaga aatactgctg
    17341 tttcattttt tgatttaccg cggctgccgt aaagaaatat agaagtgcct gcaagaaata
    17401 caaaaaccgg cgcgcagaaa tgagtgatcc atcttgtcag gaatatccat tcatttgtag
    17461 ttccaaaatc aagtgcatca tttccggctt tatgatactg atgaaaataa tcacgtgtat
    17521 ggtcaagcgc cattatgacc atcactagac cgcgtaaaaa atcaatggac tggattcttg
    17581 gttttgaaga tttttgtgaa tttgtttcca ctgttttgaa ttctggagtt aaataaaaac
    17641 aatttaccgg ttaatgctga ttacatttta aaataattct gatcaataaa ttttacaatg
    17701 gatttattac atgaatttga ttattactgc tatgaaaaaa tttacgtcgt tgctggagtt
    17761 ctaaaaagtt tgcttctcat attctctgtt ttaaaataat acttgtgcaa cttttagtca
    17821 ccatcaacta tacacttttg tcattctgag cgaagcgaag aatccatgaa tctaataccg
    17881 ctgatatcag catggattct tcactgcact cactgcgcta gtttttccag aatgacatat
    17941 aattttttag aatgagttta tttcaaaagg aagtccgaag tagattctgt tgttggtgat
    18001 caataatttt gcataatttg tttagtaaga gtcaaatgaa ctacatgcaa aatttttgaa
    18061 aacacaaaca aaataaagaa tgtaccctta gagtcacgtg atcttcggac tttgcggaat
    18121 gtctgaaact gtaattattt tgagtaaagc atcataagtt cttcgtagtt tttgctgagt
    18181 tttatgcccc ggcgggacat tacggttaca gccgttttaa cggctttttc cagtatatac
    18241 ggaacgggct tactgctgcc gtcattccat gtaaaggaat caatactctt cgaaggaaaa
    18301 ccgccgccga atatatttgc gaaaattcct gctatggtgc ctgtgttcag catagtattt
    18361 atggacgtct ttgtatgatc accgatgatg cttccgagga attgcattcc tgtttcagtt
    18421 tcttgatctc caatattaat tctgattttc gaatagttat tcttcagatc acttgtcaca
    18481 gtatctgctc ccagatttac aaacggacaa atatatgagt gtccaataaa tccgtcatgc
    18541 tgcttgttta catatgaatg aaaaattgat tcggctattt cgcctgcaac ttttgaattc
    18601 ttaccaatac tgcaaggtcc gtatattttt gttcctgatt ttaccaaagt attctttcct
    18661 ataaaaaccg gacctttgat aaaacaaaaa ggttctatta cacaattttc ttcaatcaca
    18721 atctttcctt cagaagcatc cagaattgaa cccggattca ataatgtttt tgatgaaaca
    18781 tagattcctt tatgattaac aaaattatct tgtctcttag ttggttttag tttctttttg
    18841 gaatttaaat atatctccag atcagtagtc agaccgcctt gcagcaaata ttttacagca
    18901 tcccacggat attttaaaat aaaaatatct tcatttttac cgaaatcgat tttcgaaact
    18961 tcggctcttt tgaaaaattc ttcaaaatta ataccttcag aaaatgatct ttcaaaataa
    19021 gatttgaatt cttttatttt atctttggtc agataagctg atacaacttc ttctttaaaa
    19081 taatttgctg tattcttctt tttttcggat atgatctttt ctaaactttc ctgtgaaaaa
    19141 attactctcc cgtttaaaaa aagtatatca tcttttgaga tttcattaat cctcagttca
    19201 ttgatctcac tcatcatagc agcaatagaa tttctgcaga ttagagaaac atcatacctt
    19261 ccgcacagaa tatttattat tctttcttta atgcttaccg ctccgcattt tatgtcaaat
    19321 gacgctctga gcatattcag tgggaaaagg tttttgaatt tattgtcttc gaagataatg
    19381 actttcataa cagaaattat tttataaatt aaaaaagcca atacggaaaa tattggcttt
    19441 taataaaaat gttaaatctc ttttaaatat attatgcttt tgtttctgca gtttcagtat
    19501 tttcagcagc ttcttttgaa tcaacagctt ttttggattt tccgtatcgc ttattgaact
    19561 tctctactct acctgctgta tcaatgatct tttgtgtacc ggtgaaaaac ggatgacata
    19621 atgaacagat ctcgactttt atttcaggaa ccgtagaacg cgttgtgaat aatacccctc
    19681 tgttttctcc gcagttacag ataacatcgc atttataata ttccggatgg aaattctttt
    19741 tcattttata gcttatttat taattggtca aaatatccaa tctgattcag atattcaaga
    19801 gtaaattaca gaacagacaa catatctcaa tgagtcatat aaatgtattt aaaagatttt
    19861 gaataatgtc taaatacaaa tatatttaat ttcaaaactt tgcatttgac ttaaaaatta
    19921 tttttcttac ctttggggga ttaaaaatta aaaaggaaag ggctctacaa tgattaaaaa
    19981 acatcctaca gttaaatttt tccttgtttg ttttacactt ctttcattta ttggaatgaa
    20041 agatttaccc tcacccaaag ttacacatga ttcaaatcaa aatccaaatg ctccacaggt
    20101 tattacctcg cttcggcaaa atgttaacaa cgtaagtaca tggttcagaa ctaacggttc
    20161 attcaacaga gatcctgcga ctggaaactc cggatttgaa tggccaaaag gtcaatccaa
    20221 atacgcaaga tatgcttccg gtctttggct tggaggaatt gtcggtcttg atactttaat
    20281 tgcaattgcg gaatatgatt atgaatatca gcctggttat gtggacgacg ctggaaatcc
    20341 gcagggccgt gacaatccga attacaggat atttaatttt tcggataatg atacaagcga
    20401 ttttgaagca tggcgaacca ttgcttcact tcagggagct tatctaaaca gcaacggtaa
    20461 tcctttcaga atgggatcgc aaacaatgtt ttacagcatt acagatggtt atccggattc
    20521 gcatggaaac accgcaggct ctacagctcc tctcaaagca gttattcttc agaccaactg
    20581 gtcttacatt aatgtaaatc tcaaagatgt cgtttttact gagtacagga taataaacag
    20641 aagtaatcag gcgtggacaa agttttatat atcaatctgg actgatgacg atcttggaga
    20701 tgctgtagat gatgctgttg gtattgatac ttcagctaat atgccgcttg gatatacata
    20761 taatttcacc aataatgatc aggagtatgg cgctgctccg ccggctgttg gatttacagt
    20821 tttaagaggt cccgtaactg aaagtattgg agatactgtc agatattata gtcctccggg
    20881 ttcaagcaat ctggttgtac ttccaaacat gaaagagata aaactttctt catttaatac
    20941 ttacataaat gcagatccat ctgttggaga tccatccaat tacagagaga cctatcgtaa
    21001 tttacaggga ttaagaagag atggttcaca atggataaat ccaaatacaa accaggcatc
    21061 agtttatcct tatagtggag atccggaaac atcgaacgga tggattatgc catcgggaag
    21121 tgacagaaga agtctcatgt cttttggacc aataactata caaccgggag atactcagtc
    21181 agtgattgtt gcacagttga tcgcaaaagg atctaataat ctgaacagta ttactgctct
    21241 tagaaatctg actagttatg ttcacggaat ttatcaggat aatttccagt cagtagttag
    21301 tgtaaataat tattcaattc aaactcctga caaatattct ttgcatcaga atttcccaaa
    21361 tccatttaat ccatctacta aaataagatt tgatattcct tcaaatgctg caggtcagcc
    21421 ttcgaatgta aaactcgtag tttataattc acttggaaat gaagtcagaa tacttctgaa
    21481 tgaaaaactt cagagcggca gttacgaaac tgaattcaaa ggagaagatc tttcaagcgg
    21541 agtttatttt tataagcttg agaccgacgg atttattcaa actaaaagaa tgatcctttt
    21601 gaaataaacc aaaattattt ttcagtaatt agataatttg acaccggatg caattggcag
    21661 ccggtgtttt ttttattata aaaaatgctt tagcttcaat atctaaatca ttattccttt
    21721 caattcaact taaacaatta ttcagattag gttttataat aaaggacact tcatcaaaaa
    21781 acgtgtattt ctttgaattg aataagagtg gctttatttt tattttgaat aaaaaataat
    21841 tccggacaga tttttatctg taaactcaaa ataatttcct cacctatttt tcaaaaacat
    21901 ttaaatgaca gataaaaaaa tattactcaa agcatttgct tcgatgtgta cttccaaaaa
    21961 aatggcagag atctacgatg ataataaaca gatttgtaaa tatgtacact ctacttccag
    22021 aggacatgaa gcaattcaat tagctgttgg tatgcagctt agagaatgtg atttagttag
    22081 cccgtattac cgtgatgaat cgatattgct tggcattgga ctcgagcctt atgaactgat
    22141 gttacagctc ctgacaaaaa gggacgatcc attttcagga ggtcgtacat attacggaca
    22201 tccttcatta aggagagatg gttttccaaa gatcccgcat cagtcaagtg ctacaggtat
    22261 gcaggcaatt ccggcgacag gtgccgctca cggaattcat tatttagaat ctgcaggtct
    22321 gttgaataat tctgaagata ttgaaaataa tccagtaata gtatgttccc ttggtgatgg
    22381 gtcagttact gaaggtgaag tttccgaagc ttttcagatg gcagtcctga aaaaactgcc
    22441 cattatttat ttgatacagg ataatcaatg gggtatctca gcatataaag atgaaatgcg
    22501 tgcgatggat gcttatgaat ttgccgcagg atttaaagga atgaaaagaa tcagaattga
    22561 cggttcggat tttaaagaga gttatacaaa attcggagat atgctgaatt atgtaaggac
    22621 aaaaagaaaa cctgttctta tacatgctgc tgtcccgctg cttggtcatc acacttcagg
    22681 agtaagaaaa gaatggtaca ggaatgacat tgatgaacat acgaaagatg atccgattcc
    22741 aaaactcagg aaagtccttt taaaagaagg tgtatctgaa agtgatcttt tagaaattga
    22801 aaaaaattca gcattgtttg ctgaagaaag ttttgcgaaa gcagttgctg ccgatgagcc
    22861 gtcgtttgat tcgctgacac ttcatgaatt tgcacctaca cctgttaccg aagaaaaagg
    22921 agaacggtct cctgctggaa aagagtcagt gataatggtt gatgcaggac tttatgcggt
    22981 gagagaaatt atggataaac atccggaagc tatactttat ggtcaggacg ttggcgcaag
    23041 actcggcgga gttttcagag aagcggctac actggcccaa agatacggcg ataacagagt
    23101 ttttaatact ccaatacagg aagcatacat tgtaggatct acagcaggaa tgagtgctgt
    23161 cggtgtcaga cctatagttg aaattcagtt tgctgattac gcctgggcag gaatcaatca
    23221 gcttgtggta gagctatcaa aatcatgtta tcttacaatg ggaaaatatc ctgtatcttg
    23281 cgtgataaga attccatgtg gtgcttacgg tgccggcgga ccgtttcatt ctgattgtat
    23341 tgaatcgagt cttctgccga taagaggtat caagattgca ttccctagca atgctgccga
    23401 tatgaaaggt ctgatgaaat cagcttttta tgatccaaac cctgtgatta tgcttgagca
    23461 taaaggtctc tactggagta aagtaccggg cactgatgct gcaaaatgta ttgaaccttc
    23521 agaagattac atcatacctt ttggtaaagg aaggatattt caaaatgctt cggaagataa
    23581 aataaattca ggtgaatcac tttctattat tacttacggt atgggagtgc actgggcatt
    23641 gaatgcatca aaagattttc cgggatctat agagatagtt gacttgcgga ctttaaatcc
    23701 gattgatgaa gagctgatgt atgaatctgt taagagacat ggtaaatgta ttgtacttac
    23761 tgaagagcag gtgttaaatt cttttgcgga aagtctcgca ggcagaattt caaaaaattg
    23821 ttttcaatat cttgatgcgc cggttgaagt ggtcggttcg gttaatatgc ccgcagtccc
    23881 tctgaattca ggacttgaat ctatgatgct tccgaataaa gataaagtat caaaagtgat
    23941 tggtgaattg cttggttatt agattaatta tcaggcggtg gaggtggggg atcagctcct
    24001 tctttttcag cttcttttaa catttcctgt atctcttccg gtgttagctg atcttttcct
    24061 acatctgtac cagtctcttc aggatttaca tcagtatttt ctttattttc attttcagga
    24121 tttacaactt cctctttttt ttcatcttca ttatttattt ccgtaggagc gacattgtta
    24181 gtatcacttg catcttttaa tgaatcattt ccactattcg tattcaaaga atcatttgca
    24241 ttattttcct ccagtgcagc aaaataatct aaaagttcag aaattttttg agtataaata
    24301 gattcaggat attccgatcg gagaactccg taataataca gtgagctatc tttgttcaaa
    24361 gatccgtttt caaaaacaaa accaataccg taaagtgctt ttgctttaat ggaatccagt
    24421 tttgcatttg atctgatact tttcaataga tctacagctt cagtatattt ttgatcatta
    24481 taaagctcaa gtgctttttt aaaatcaata tcagcagtac tagtcactac atcatcttca
    24541 gttataactt caccaattat tttttttgct tcgattgcat atattgtatt tgaataatct
    24601 ccgataagct tactaaatgt ttcctgagct tttacatctt tacctgttga tttataaaaa
    24661 ttaccaagcg tatatagtac ttttgattgc tgatcaggct ctttaaactt ttgaagaatc
    24721 agtttcagat aaaattcagc tgaatcactt ctgtctagct tatatgtaaa taactccgca
    24781 agttcataat atccattaaa ctctttttca atttttgttt taagatctat ttcttcctga
    24841 acctgtttca gcgagtcagt atttacaaca cttgtatcat tttttgattc agtatcattc
    24901 ttttcagtct tgtcctgatt tcccggatta aaattatttt cattgttttc attcttgttt
    24961 ggactattat cagatcccga atttttctga tcatcagatt gttctttatc ccattttcct
    25021 tctttaattg gatcttcggt agaatctctt aaattttcaa aataataatt attaaatcct
    25081 ccgggtcccc ctttgccgtc acctctctga tcaccgtcat tttcatcacc ggatttatta
    25141 ttaccttcat tgttattacc tcctttatta gtctcattga actgttctat gcctttttct
    25201 tcattgtaaa atgctctgta cttttcaact tcaaaatttt cctgcggaat gtcaatgggc
    25261 tgtgaatcag ttacttcact ttgaagtgta aaatatctgt cgagatctga aactttttca
    25321 gaaactaata tataataatc agatgatgca ttttcctgta ctgatttttt ataatttacc
    25381 aatgcattca gataatcatt ctcaatttct tcttcatgtc ttcctataaa aaaatatgca
    25441 tcagctgaag cgacagagtt tggatatttt acaatcactt cgtaatactt atccatggct
    25501 tcttttagtt tattctgagc atataaatta tttgccagct caagatccac aagctgggca
    25561 taaccaattt catctctgta tttttttctg agactggtca gctcttcatc tgcaagacta
    25621 aactttttat tatatgtaag accttttgca taatttaatc ttccataaaa tgaaagatcg
    25681 aagtctgatg tatagtccag aactatttta tattcgtttg cggctagttc gggcttaaag
    25741 agagaaacta attttgcaag tataaattga ctttcggctt ttctgacatt atcattagta
    25801 tattcgatgg cttttctgaa atattttgcc gcctcttcag gtctcccttt tttatattca
    25861 agcaccccgt aatctcttgc tgccagtgat ttgatctccc cttcagtttc attttttaaa
    25921 agactaaggt aaatattctc agcttcattt tctttgttca atttattttt tgacctcgca
    25981 agataaagaa ttgcttcatc agtcaattca ctgctggaaa actttgaaag aaattcgttg
    26041 aactttcttt ctgcattaat gtaatcagat aaaaaataat atgattttcc tatgagtaga
    26101 actgcatcgt cgatatattt actgttctta tggaattgaa taatcttaga acttctttca
    26161 attgatttgt taagcttatc ctttacattt tgagatgcag gcgggtttac taaaagtgaa
    26221 tctatacttc tgttaaatat tgcaatgaaa gacaaacgga attcatcaaa agcttcatcg
    26281 taatcctgct gagctttaaa atatgtatta aagtatgccg tgaaattttc gatccggttt
    26341 cccatataca gatatgaagt aaaatcctga tagtcaaaat tcaaagatac atcataatct
    26401 ggatcaggtt ctgaagatgt cgaatctgta tttgaatttt tagttactgt agtgtaagta
    26461 agataactgc agctattaag atatactgca aatactgcaa ctgaaaaaaa tattattata
    26521 aatctgctga tcttcaagat gagtttattt tgttaaaaat atttttaata tgaagtttaa
    26581 tcaagacaat tataaaaatt gataattgtg taacacatca cctttataaa tggtttattt
    26641 acaaaggtta ttaaaaataa tgtgccggaa ggatattatg attaaatcaa tttagtattt
    26701 taaaaagtta aaaattattt tggaaataaa ttaataaaca gtaaatctaa ttttctttta
    26761 taaaagcgca taattaacta atttagtgct tacacatctc aataaaaacg aaaacctaat
    26821 tttcaataaa tgaaagatac taagttattt acactgctaa gaagtatttc aaaggaagag
    26881 tttacggaat ttcatgattt tataatctca ccgataatta ataaggatga aaattttata
    26941 attttgtatg attatataaa aaagaattac agcttagcac tcaaagatga aataggaaaa
    27001 gaaaaaattt ataaagccgt tttcggaaaa agtgatttta atggatccaa atactggaaa
    27061 ctaacttcgg gattttcaaa acttatagac aaatatcttt tatttacagc atttgaaaat
    27121 gacccttttt atcataaaaa tttgctgctt gatatatacc gtacgagaaa cgtacataaa
    27181 caatttgaga ctttgtcaaa agagatccac agaagtttta caaaagaatt taacaaagga
    27241 ttaagttatt atcttaacag gacacatttt tattttcaga aactaagcta tctaggacct
    27301 gagaatattg aggaatttga agatgacctg aaaaaaatgt ttgaaaatct gaaaatgttt
    27361 tttataatga caaacataac cagtgtcagt attatctcaa acttcaaaga gaaattcccg
    27421 gtagatgcaa aaaaagatgt gtggatgttt aaagaaatac ttgcatactt aggaaagaac
    27481 aaacagaccg tgaagaatga agatcagatg gtgtatattt tttatctgat catattaatg
    27541 aaaatggatt acagtgatgt acaaaattat tttgaaataa aaaaaattat aaccaccagt
    27601 aaattaaagt tttctaaaaa cctgctcaga cacatactta taaatctttt tgattacgca
    27661 gtaaaaaaat ttacaacagg tgaagagaaa tttcttaagg aaatttattt tataaataaa
    27721 attatggatg aaaactctgt aactctgttc ggagaattta ttcagggaga gtatttctat
    27781 tcagtagtag agcactccgt aatgttgaat gagatcgaat ggacagagga atttatacga
    27841 aaatatggaa aatatcttac cgatgattac agggattcag ctttaaatct ttccagagca
    27901 aggataaatt ttgagttaag tgattttgat tcttctctga atgatctgct gaatgtagaa
    27961 aatcttaatc cgtattttta tctttcacat aagatcctat tgcttcaaaa ctattttgag
    28021 attaaagatt ttgaatcaat cgacaggtta cttgaaacca cgcagaaata tctgaacaga
    28081 agaatagatt tatctgatga attgaaaaaa aattatctca aattttttga ttatttcaga
    28141 aaattaaaaa cggcggctac tgtaaaaaga tacctatcaa gaaatctata taaagagctt
    28201 gagaaggaat ctttttttat ttataataac tggcttatga taaaaactaa agaactgatc
    28261 tgactgtctt ttaaactgaa tcaaagttta aaaaaaagaa aaaattatct aatgtaaatt
    28321 ttacaaaatt aacttaataa aaggaatagt attttatatt ttgaaaaaac ttttttaaaa
    28381 tttattaatt gttgatatgg attctttagt aatactttct ctaaaattag ctgtcggtct
    28441 cataatgttt tcacttggac tgaatagtac gatgagtgat gcagcatatc tgctgcataa
    28501 tccaaagctc cttataaaat cgatcatttc tatgaaccta attatgccgt ttgttgcagt
    28561 gacaatgatc ttaactcttg gattgaaaca agaagttaat cttgctttgt ttgcactggc
    28621 aatctcacca atcccacctt tactacctaa taaagctttg aaggctggag gaaaaatctc
    28681 ttatactgtc agtttacttt ttataatggc actcctttca attattttta ttccattgtc
    28741 agtggaatta atgggtatcg ctaatttaaa agacttttat gtatcgcctc ttgtagtttt
    28801 aaaagtaata ggtgttactc ttattggtcc attattattt ggattaataa tcaataaagt
    28861 cgcgctttta tttgctcaaa agatcaagaa agtagtttcg atcactgcaa tgattcttct
    28921 gattgcgggg ataattccat tacttatcaa attgatgcct attgttataa ccttattcgg
    28981 gaatctgact attctttcac ttttaatttt tattatttcc ggacttgttg taggacactt
    29041 acttggtgga ccggtagagg atgacagaac tgttctggct ttgacaacat ctacgcgcca
    29101 tccgggaatt gcaatagtga tctgcatgac agtaatgcct gaggtaaagc ttgctgtttc
    29161 agttattttg ctttatctca taattaattt gatcgtgtca attccttata ttaaatggcg
    29221 aaaaaaaacc agttaaaatt tttttcatca aggtattaaa tttatataaa atgaccggga
    29281 gtttattacc acccggtctt ttactttttt aattaaacaa tttaacttca gtttcttttt
    29341 ctactattac tttttccgga tctgttgttt gatttgcctt tattgctgtt tgtatttgac
    29401 gagtttgatt ttactttatt ctgaccgttg tcttttttag tattgttgtt ttttatattt
    29461 cctgagtttt tagtaccgtt gttttctttc tttccattat tacccggctt ctgaattttc
    29521 tcaggacttt cagtcttggt tccattattt tttggaggac tgaatttttt tatattagtg
    29581 cttttggtat tgggactttt ggtagtatta ttgcttttcg tattagggct ttttgtagtg
    29641 ttagtgcttt tcgtattcgg gtatttagta tttgtgcttt ttgtatttgg atcattagtg
    29701 ctttttacat tctcattttt tgtggtaaca ggattattta attctgattt ggttacagaa
    29761 tttttagtgg aagtataatt tactccctca tcatattttc cggatttagt agtatttatt
    29821 gagacatatt tcggagtaat cacttctccg gattcttttg tgacctgttt aacatccggt
    29881 cctgtatatt tgaaatcttt tgtttcttta tcggtgtaag ttgaaatatc cattttagtg
    29941 gaattattca gaatgttaac gttatcaacc ggatcaccta caactttttc atctattctc
    30001 tttgtgacat ccgaatattt tacaactacc caattatgag gggaataact gtataatgtg
    30061 tttccgcaat atccatttcc ataactatac cagtagatac ttgggcaggt tggataccag
    30121 gcataatagc cgccgcaatc ccgccatgag acccagtttg cagcccatac atttccgggc
    30181 agccataccc agccgtaata actgctgctg taccatctgc cgtaattata aggtccccat
    30241 ccccaggcat aatccgacac ccaagtccat ccgtaatatg aaaataccca tctgccgcca
    30301 atgtaaggat tccagttatt gtctgctaca tatggctgcc atacataaat atattctcca
    30361 ttatcagaat aatagtattg aaggttttcc ccggtttctt cggtgagttc ttttatgaag
    30421 tcggactttt taactttgat ccagtcaccg tcttctgaaa gttcttcgaa ttgctgtttg
    30481 taaacttcat ttgaatctaa aagattttct gaagtctcga tcgtgtcaac aaatatttgg
    30541 ttattgtctc cgctttcgtt gggtatttcc tgcgctttgg attcaaaggt gaagatcaat
    30601 acaaacagaa gtaaagacaa caccgctgac aaaaagtttt tagttttcat ggtgatcttt
    30661 ttttatttta aaaatttatt cggcacttgt acaaatataa cattatttag ttcctatcgc
    30721 aaaaaaataa aactgaaggt tgtttcattt tatttttaca ttaaaaattt tgtattactg
    30781 ccggggaaag atgttttgaa cttaagacac tataaattta aaatgggttt aattaaattt
    30841 acttaaatga aaatttatca aatttccctt cctatattca taataaataa atttcagata
    30901 atttgaaatc aaaaaataga attgataatt tttggagaaa tgaaggttga tctggaatta
    30961 atttatgatg atccatttga gagtaatgct tttaatattc ttgtatggac agaaagtaag
    31021 gtctaagata attgttattt ggatgcagca aaagagattc agtaaagaaa atgatttcta
    31081 taataatttg gatttctttt tttaataatt ctgataattt ataatgaaca aaaagcggtt
    31141 ttattatttg gggctgatag tatttattat catactcgga attgcttcaa ggaaatattc
    31201 aacactgtta ccggattttg tttcagatta ttcaggtgat accttatggg cattaatggt
    31261 cttcttcata ttcagattta tttttccaaa acatacaata ttttatactg caattatttc
    31321 gttgatcttt tcatttgtaa tcgagttcag tcagttttat caggcagaat ggttaaataa
    31381 tataagaaat ttaaaatatg cagggttatt atttggatat ggttttctcc ggagtgatct
    31441 ggtctgctat acagccggta tattaattgg atttctcttt gaaaaatatt ttaaaaatta
    31501 aattatttta ttatgattaa cacaaaaaac gaattcacgg aagagatgta tcagtatggc
    31561 aaatcactga tagaatgtct cgataaagaa ggttttatat atccgcttgt aatgtggata
    31621 aaatttcctg ataaggaaga atggtcactt ttatttggaa tacccggact gaaaattatt
    31681 ggcagtcctg atatccttaa taacatgaaa aggatcatta ataaaaatga gttaaatatt
    31741 tctgctgaaa atatcagtct gatagattct atggacgagc tatttattaa cattaaatcc
    31801 tgtatcagat tgagcggtat ttccagaatg aaggtctcat ccactcaaat gaataatatg
    31861 atattaccgg aaagtgttta ttacagagtg tcttgaatta actcaaatag gtaaatatta
    31921 ataaaattca cgtttttgga tatatccaat tatatcaact ctattagatt aggattgaaa
    31981 ctatttctat gtagttttat attattacca ttctatataa attagataaa aaaagaactt
    32041 aacgaacaaa ttataatggc aaaaagcaaa gaagaaaaaa tagattttac agaagtagat
    32101 gaaagcattg atgaaaattc ctctgaagta atttatcaga acagttcaaa tatcccggac
    32161 ttaaatgttg caataccgaa agaccttccc gtacttcctt taaaggatat ggtagtattt
    32221 ccatatatga tgtttccgat tctggcaggc agagaatcta cgatagccgc tatcaacagc
    32281 tctctggaga aagataaatt cattatgctt gtttcacagc ttgatgaaaa aatagaagaa
    32341 cctacttttg aaaatctgaa taaaacaggt acggtagcaa agatcataca ggttctcaga
    32401 cttcccaacg gacttataaa agttttagta gatggtcttg tagcagcgaa agtaaaaaaa
    32461 ttcaaaaacg ataaatatat ttcagccgag gttgaggttt taaatgtaca gttcaaaaaa
    32521 gacacagagc tcgaagctct catcagacag gctacaaaat tattcaccga atatgtaaat
    32581 acaaacagga caattcctca ggagacgata gttgcatttg aaaatataaa agagccggat
    32641 agaaaacttt tttatattgc atctacaatt aatgctgacg tagcaaagaa gcagaggatt
    32701 cttgagctga ctgacattca caaacagttt tacgaaatac tatatatact aggctctgaa
    32761 atggaagtac tgaatgtgga aaaagagatc gatgcaaaga tctatcagac aatgcagaaa
    32821 aatcagagga agtttatagt acaggagcag atacgtattc ttcaggacga acttggtgaa
    32881 ggaatggaaa ccgatccgga tctcataaag attcgcgaac agatcgagac aagcggaatg
    32941 ccggagccaa tattaaaaaa ggcgatggat gaattcaata agctaaagaa aattccttca
    33001 atgtcgcctg atcattcagt aataagaaat tttctcgact ggatggtaag cgtaccgtgg
    33061 aataatttta ctgaagataa ttttgatctg ctgcatgcaa aggaaattct ggatgaagat
    33121 cattatggac tagagaaagc taaggacagg atcatcgaac ttatggctgt ccttaaactt
    33181 ttagatgaca aagatattaa gaaatccccg aaaggtcaga tactttgctt cgtcggacct
    33241 ccgggagtag ggaaaacttc tcttggaaaa tcaattgccc gcgctattaa tagaaacttt
    33301 gtcaggattt cagtcggcgg agtgagagat gaagctgaga tacgcggaca cagaaggaca
    33361 tacattggct caatgcccgg taagataatt caggcaatga aaaaagcagg cagcgtcaac
    33421 cctgtgatac ttatagatga gattgataaa atgagcagtg atttcagagg ggatccgtcg
    33481 agcgcaatgc ttgaggttct tgacccggaa cagaattcaa catttaatga tcattattta
    33541 gatgtcgatt atgatctttc caatgtgctg tttataacta ctgcgaatgt ctattacaat
    33601 attccgcttc cattgctcga caggatggag attatagaaa tgcgaagtta tctggatttt
    33661 gaaaaattac agattgcaaa gaagcatatt attccaaagg aaattattga acacggactt
    33721 actgaaagca tgatcaaaat tcctgaccct ataatattgc ttgtcattca ggaatataca
    33781 cgtgaagcag gggtaaggaa tctggaaaga gaattatcat ctgtgatcag aaaagtaaca
    33841 agagagatcg tagaaaaagg taatcctaaa aagagtaaaa cgattgttat taccgaagag
    33901 atggtaagga aatttctcgg agttcaaaag tataagcaaa agctcgctga cgataaaaag
    33961 tctgaaaaga tcggatctat agttggtctt gcatggacat caatgggcgg tgagattctg
    34021 catgtagatg taaatataat gaaaggtgca aataaattta tactgacagg aaagctcggc
    34081 gacattatga aagagtcagc tcaggcggga ttctcttata ttaaatcaaa tgcatctgat
    34141 ttcaaaattc ctgaaagttt tttcaaggaa aaggaaattc atatacacct tcccgaaggc
    34201 gcaattccaa aagacggacc atctgcggga atcactatga tcatggcaat gctgtcggct
    34261 attacaaaaa atcctgtgcg gtcggatgtt gctatgacag gggagatcac tctgagagga
    34321 aatgttcttc caataggcgg attaaatgag aaattactgg cagcagtgag aagcggtata
    34381 aagacggttc ttattccggc tgataatgaa aaagatctgg tcgaagtacc ggaagagatc
    34441 ttaagtaagt taaagataat ccctgtggaa aaagttgagg atgcaatttc tcatgtattt
    34501 aaaaataaaa atgttatttt taaaaaataa attataacgg cctttcaaat gaccgtaaag
    34561 aaaattgtca gactataaag tatcagcgcg aaagtaccgt cctcagaaat tctctgaagt
    34621 tacatcacag gaattcgtaa cacagacaat gaaaaatgct atcttatcag aaaagatcgc
    34681 acattcttat ctgctcagcg gtccgagagg agtcggaaag acaaccattg cgaggatatt
    34741 tgcaaaggca atgaactgta cggatctcaa agacggtgaa ccttgtaacg tctgcaactc
    34801 ctgtgtggaa ataacaaacg gaaatcatcc ggatgtgttt gagctggacg ctgcatctaa
    34861 cagaggtatt gatgatgtaa gagctgttca ggatgctgca aagttttttc cgataaaggg
    34921 gaagtataaa ttttttattg ttgatgaagt tcacatgctt acaactcagg cattcaatgc
    34981 gctgctgaaa attctcgaag agccgcctga atatctgata ttcattctcg ctactacaaa
    35041 tcctgagaaa atacctgcga ctattgtaag cagatgtcag agatttccgc tgcagagaat
    35101 taagatcaat gaaatttcag ataagataaa agagattgca aaagaggaaa aggtaaagat
    35161 cgatgatgag tcagtatttc tgatcgcaaa actcggagac ggtgcattaa gagatgctga
    35221 gggtgtattt gatatggcgg tttcattctg cggagataat attatataca gagagctcaa
    35281 atcatttctc aatattcctg ataaggatat ttattttgac atgtcagagt tcataaagaa
    35341 cggcgatgta aaaagcatac ttatatattt caatgacctt gaggaaaaag gttttgatat
    35401 gcagactttt ttcaacggca ttactgaaca tattcgtgat ctgctgatag taaaatcaac
    35461 ttcaaaccct gaactcttag atgagtcgga ttcattcagg gaaaaatatt ctgttgcttc
    35521 caaagatttt tcgaccgacc agttgcatag acttttgaaa atactttttg aatcagaaag
    35581 cagattcaga tattcagcta atcagaaaat attacttgag attatacttg ccgaactttg
    35641 tactgtaaac agggatgtgc aagacttgtc ggagctgatc tcagaggttt cctcattaaa
    35701 aaaaaaatcc ctgactgaag atctgagtga aattacatca gaagatattc ccaaaaagga
    35761 tttatacaaa actgataata ctgaatctga aaatcagata aagaatgaaa ttgaaacaga
    35821 taaaaaaaca gatcaggaag cggataaaaa ttcagagtat atatctgaac aaaatacagt
    35881 cacagaaact aaaaataaaa agcaatctga ggagggatca tctgaaataa ataaaattga
    35941 gaactcagaa gtacatgtta atgaaaactt tgataaaagt cattcatcaa aatcagaaaa
    36001 ggattcagaa ttagtagaaa agcttaaaga gctttttgat gtagaagaat ataaaataaa
    36061 ttaaattgga aaaaagaata aagttttacg gagagaagaa aaacttttta gcaatagaag
    36121 aaaaatattc ggattataag aattcgggga tagtaatttt acaatctcca tacgaactta
    36181 ccacttcata cggcaaagga actgcagaag gaccaaagtc aattattaaa gcttctcatt
    36241 atgttgaatt ttttgatgaa gagctggacc gcgaactttg ttttgaaaaa ggcaacggta
    36301 tctgtacatt gcatccgctt gattttaaag ataagaaaag ccgtgaagct ctggattata
    36361 tttatgaaaa tgtaactaag catattgaaa atggaaaatt tgtcgttctt ctcggcggtg
    36421 aacattccat atcaactgca cctatccgtg ctcattttga taaatatgaa aatatgtctg
    36481 tgctgcattt tgatgcacat tcagatcttc gtgaggaata tgaaggttca aaattttctc
    36541 atgcatcatt tgctgcaagg gtggcggagt ttacaaaaga tataacacag gtcggcatca
    36601 gagctcagtg taaagcagag tatgatttca taaaggaaaa cggtatcaaa actttttatg
    36661 cgcatgagat cagaaacggg acatacggtg atgactggca gagtaaagtt attgaaaagc
    36721 ttaaagaaaa tgtttacata acttttgacg tggattattt tgatccgtcg ataatgccat
    36781 ctacgggcac tcccgagcca aacggttttc tatggaatga aacaatgaaa ttattaaaaa
    36841 tgcttggtga aaaaaagaat atagtcggct ttgatgtagt agagcttgct cctgtaaaag
    36901 ctttttcgta tccggacttt atgaccgcaa agcttgtgta taagatgctt aattatttca
    36961 gaaagtgata ttaattctga gaaatgtaaa ttttaaataa aaaacttccg gcgtaattat
    37021 cacaccggaa gttttttatt taatatttaa aatttaaaat ttatttcaat actgtcattt
    37081 tttttgtaat tctaaagtca tttgacaaaa gagtataaaa atagattccg cttgacaatt
    37141 cgtttgcgtt gaagtccatt acataggatc caggagtcat ttttgaattt attggttcgg
    37201 aaattactct tcctgaaata tctgtaatag tcatttttac attacccgct tttaatatat
    37261 caaatttaat atttgttgta ggattgaacg gattgggata attctgatga agcataaatt
    37321 cacccggtat ttcatttgaa atattttgaa ttcctgtcga ttgtgaatac ttcacagaag
    37381 cataatcata actggtaccg cttccgccat aacttaaccc tgtgacaacc acaaattcct
    37441 gattatttat agctattgaa gttgcagcat caagtccgct tccgtttccg ttgtatgtcc
    37501 tgacccattt ttcaactccg gaagtatcgt attttactga tgcataatcg aaatttccac
    37561 tgttcagact agttcctgta atgtacaatg agttatcaat ccctataacc attgacttag
    37621 gaacatctct accattgccg gatccgttaa ggcgctgcag ccattgctga gttcctgctg
    37681 aattatattt taatgtaaca taatcttcat tagtaccaac gccatttgaa gaaccggtaa
    37741 cataaatatt attttgatca tctgttttaa cactaactcc gaggtcgtca ccgtttccag
    37801 ttccgttata accgcttgcc caaagcagat ctccgttttg attatatttt atagtattat
    37861 agtcatagcc tgaattatta aaattacttt caccggtaac aataatattt ccgttattat
    37921 cagaagttat acttcttcca aaatcataag aattatcagg accattatat ctcctgatcc
    37981 agttagcatt accattctga agatacatta tagttattac atcataaccc gatcctgttc
    38041 cttcacttcc accggtaaca taaactaacc cgttgttatc tgtatgcata gataccggaa
    38101 aatcatcacc atttcccgta ccgttgtatc tgactgcata ttcatttaca ccatttgaat
    38161 tatattttaa agtggcaata tcataggaag aacctcccca gctaatccca gtaacatata
    38221 catttccgtt attatcaata ttaataatta caggattatc tattccattt cctaaaccgt
    38281 tatatgatct aagccaaagc tgatcaccat ttgaattata ttttattgta acaaaatcac
    38341 tgttagatgc tcctccctga ctttttccgg taacatatac atttccggaa ttatctaata
    38401 caattgaatt tgcttcatca atacctccaa cattgtcata tcttttagcc caaagctgaa
    38461 ctcctcctga attatatttt acagtggcga aatcataatt tgaagctggt aaaaaacttt
    38521 taccagtgac atagacattt ccgtcaatgt ctattgccga tgaatatgct tcatcgattc
    38581 cgtttccgga accattgtat ctttgtaccc attgttgtga aatttgagaa ttggcatttt
    38641 cttgtgtaaa tattaaaatt actataaaaa gtaaaaatag tgatttcata atattattat
    38701 ttaatttaat aaaaatttct ataaagctat gtaaaacaaa gtaattaaat aattcctata
    38761 aataaacact ttaaatatat cacatttaat ttattgtttt taccaatata ttgtaaattt
    38821 aaaagttttt taaaaaaaga tatatacagg acttgtaaat ttaaacccag tcgtgtattt
    38881 ttgtcaccta tggtcacaat aacaatttta tttctgatag taatttctat cgtattaatg
    38941 gctgtcgtat taatgcagtc atcaaaagga aacggtttat ccggtcctat tggaggatcc
    39001 agcgtcacta tggcgtttgg agtcagaaga acttctgaca tactctcaaa aacaacttca
    39061 gcacttgcag ttatattaat ggtaggttgt atagttgtaa atctaatgat tggcggaagt
    39121 ggtgcagtcg atgaaagcat tatacaaaaa aatgcagggc agcagcagca gcagcttcca
    39181 ccaaatacaa ttcctccgga tcagaatgct cctcctcagg gagagacacc tgatcagaca
    39241 cctgatcaga cacctggaca gaatcaggaa actgataaaa atgctgagag accacctgtc
    39301 aatgttgagc cagaaggtcc gtaaaaaatc ttaaatttta atttacatta aaaaatttta
    39361 ttttagcact atgaagatat ttcatagtgc tatttttgtt ttatttggag tcatattttt
    39421 atttattaat agggctgatg cgcagatcaa aactgataaa atttcatttt atgatttgaa
    39481 ggaaaaagta attagtaaaa atgatacttt aaaatcaaat caggaaacga aacaattatt
    39541 cataatgaaa aaacagccgt ggagagctgt tgcttattca gcaatcctgc ctggtgcagg
    39601 acaattctat tctgaatctt actggaaagt gccaattata cttggactgg gtggatactt
    39661 tgtttatggt ataatcaaca ataataatca gtatattgat tacagagatc agtatcaaaa
    39721 ttctcagaca accgaaaatc ccaacggtaa ccaacagctt cttaatctac gcgaatttta
    39781 cagagatcag agagataatt tttatatcta ttctgccatc ctttatcttg ccaatttaat
    39841 tgacgcttat gtggatgccc agttgtatga tttcaatgta tcagataaaa ttaattttgg
    39901 tcttttgaaa aagaacagac ttattggaat cgaatataat ttttaatatc caaacacatc
    39961 tttaatggta agtactttta cctttccata tttttccaaa actttcatat caattttctt
    40021 tgtatcagcc aaaacaagta ttgtatattt gctgtccttt acattctgtt tctgaaaagt
    40081 aacaatatca ttcagtgtga atttatcaat attttcatag atctttttat caatattttc
    40141 ggtaattccc aactgaagat tattttcata ttctccaagc agacctttag gcgaagttct
    40201 ttcagactgt atctgtttta ttaaagtact ctttgcatta ttaaaagcaa cttctgatgc
    40261 agggaaattt tcaagcagat cagtcatacc cgccatagct tcaggaagct tatcggcttg
    40321 tgttccaata taggcaatgt tataatgtga ataattattt tttttcggca tttgaaaaac
    40381 tgaaaatgta gaatatgcaa gagcttttga ttctctgatt tcctgatata ccacagatcc
    40441 cattcctcct ccgaagtatt cattgtacat cgaagcaata ggaactattc caagatcaaa
    40501 tggtcttttt ttagaaataa aaacaatctc tgcctgctgc atatcataat ttactacata
    40561 gacaatattc tcctcagttt cttcctcttt aaatttattc aaagttttta aagtcttcag
    40621 ctctgaagga gttttatgat cttcatttaa tgaacttatg atctcactat tgccaagcgg
    40681 accatagtaa taagctcttt gtttaaatga aaacatatcc tttattattt cagtaatttc
    40741 ctcacctgta acagaattca attcttcttt tgacagacgg taagtgaaag gattatcttt
    40801 tccgaatttt ccatagttat aaagcccact ccagaggata gtacttttat caagttttgc
    40861 atcttctctc tctttattaa tatctgattt taaatttgtc aaagcttcac tgcttacttt
    40921 agggtctgat ataatttcct caagaagttt taatgactca tcaaaatttt cattaagccc
    40981 ggacagactt actgagagct gatcctctga gacagaaaca ctataactgc tagccagccg
    41041 gtaaaatttt tcttttagtt cagaagagct atattttgaa gttgctatat agtcaaagaa
    41101 tgctgttgcg agtgcaattt ttttattctg atttgtgcct acttccgaaa taaaattcag
    41161 ataaaaaaga tcgttctctt tattttcaac ataaaacaaa gggatgttat tgtttaaaga
    41221 aagtttggta atgtctttgt cgtaatcagc aaactcaggc tgtatctctt cataagacat
    41281 attacttatt tcctttataa aatcagattt gtcatctctg tttattttca caggattaat
    41341 ttcaggcttg tcaactttta caatggattt atcttctccc gttctcttat aaacaattac
    41401 ataattagta ttcaaatttt catttgcaaa ttttactatg tcatcttttg tgatcttaga
    41461 caatctgtca acattgaaaa tatatttatt ccagggaata tcgaggatga atgattttaa
    41521 aaaggaatat cctcggctgg aatttttttc ataagattga agctgattct ttttaaaatt
    41581 atttatagta gcttcaatga gccattcagg aaattcacct tttttaacaa gttcaagctg
    41641 ctgaagcaat agatctttta cctgatccag agactgacct tcccgcggct ttccgcccaa
    41701 atacattaaa gaataatctt tattattata agtgccgcaa taagcatcta tcactttttg
    41761 atcctgattt agattcagat caattagtcc tgcagatccg ttgcttagaa tatattccat
    41821 catagtaaga atatctgcat cctttgtatc tgcacccata tttctatatc cgaacatgac
    41881 tgactcttca gaaggtccaa ctacttcctt tattaccggt ccattgattg gatcttgaac
    41941 aggtgaaaca aattcgggat ccggtttgct ttcaaatttt ccaaatgtat tattgatcag
    42001 ctcaatagtc ttttcaggat caagatcacc tgaaagacaa atagccatat tatttgtcac
    42061 ataattttca ttgtaaaaat ctattatggc ctggacagac ggattttgta attgctctac
    42121 gctgcctata gttgtctgtg tgccgtatgt atgactcgga aaaagtcctt ccataattgc
    42181 ttgccaaact ttatcgttgt catcatccag agtagtattt ttttcttcat atacagcttc
    42241 gagttcggta tgaaacaatc tcatcacagg atttctgaat ctttcagctt caagtgtcag
    42301 ccatctttcc agctgattag ttgggatatt acatgtatat actgtttctt ccaatgaagt
    42361 ccatgcatta cttccgtcac ctccaatgtc agcaatcatt ttatcatatt catttgcaat
    42421 ggcatattct gaagcaacat acgaaatgct gtctatcttt ttataaatta attttctttc
    42481 atcaggatca gttgctagtc ttctctgctc atataaagca atgatactgt caattaacgg
    42541 tttttcagct tcgaaatttt ttgtgccgaa tttatccgta cctttgaaaa gcatgtgttc
    42601 cagataatga gaaagtccct gagaagtcgg aggatcattt ttgctgcccg agcctactac
    42661 gatataagtt tgtattttag gttcgacttt atttacagat aaaaaaactt tcagaccgtt
    42721 atcaagagta tagatccttg ctttgagcgg atcgttttcg acttcttcat atgtatattt
    42781 tccgtctgtg acttttttta attcctgaga ataaaggctt gatgttacaa agattaatat
    42841 tacagcaata aaaatattta tcattgctaa agtagttttg aacttgctca taatgaattg
    42901 ttgaatttta atttagataa caaatttaat ttttatgaat ttaatttaat atgtaaagaa
    42961 tgttcttttt tgaagctttg gaagttacgg aattttttgc tatgggttat taaaattaaa
    43021 cctaaaatga acttgcattg tcctaactcc gtaattcaaa aacactaatc aagaaaggat
    43081 ctgataaaat gaaaaaccaa aatttttata aattgaaata ccctcttctc attgcattat
    43141 ttctgacagt aataatttct tcatgttcag ataatacaat tgaaaatggc gcattgcaga
    43201 ctgatgatga atacatcaga aatattgcaa tgaactcatc attcagcagt aatgaggacg
    43261 atgatgataa tcttttttat tcagatgaaa tggattttga gtctgaaggc gcaacttaca
    43321 gcaatgcaaa caacaacacc cccctggatt cagttttaaa gtggggaaga agaattacag
    43381 gaaaaaatgt aaatgtaaat attacaactc atggtgatac tttaaaagat gtaatagtta
    43441 caaaagcaat aagcggaaat tttataatca ttggttacac tggcggaatt cttgactctg
    43501 ccgtaaaacc tttttcacag gagcacaaaa gactgatctc ttttaaaaga gttgacagaa
    43561 gacaacatcc aagacataac tggagagtct ttcaatattc agcagtagac ggagagacaa
    43621 agacacctca gatcggaaaa gaaaatatta caatgaacag agttgaatat tacagaaata
    43681 atgaactgat acttactcta aacggaccag actttacaac taatatattt tcagcaagac
    43741 attttggaaa tcagtcacag atagaactcg tcagaggtga tcaggtaaaa ataaaagttt
    43801 ttctttcaag caatcaaatt gatgaagaca ttgttgccta tcactggggg agaaattcac
    43861 acggttttca cagagaatta tttgaaatga caacagaaat tcagaacgga aataattttg
    43921 acaggacttt cgaaaaaaca tttgaaatat atcaaaatca caaacacgga atgcataacg
    43981 gatttataag ttctataaca agaagttcat tatttgataa ctcaacttct ttgttctctt
    44041 caacgtacat ggggttaccg tacagagtca gacactaaac ctcctgaata attttgatgc
    44101 cgaagcagtt tttctgaaaa gaagactgct tcctctgttt atgaaccaca cagtcacgta
    44161 gaattaataa attttaaata ataaatattt gaataaacaa ctttcaggaa attatatata
    44221 gaaaaatctt aattggaaat cgttttaaac aatgattaaa aatggatatt ggttttcaga
    44281 aaaaatgaaa ctatttaaaa tttataattt aaaattaaat aagtttttcg ccgaaagaaa
    44341 tatcgtaacc gtcaggatcg gacactccaa attccttgat attataatca gttacataca
    44401 atgaataatc tatctttgca cctcttacaa taaattctct gtaaattttt tcagcatcat
    44461 taacccaaaa atatacatta cttgttttat cttttgattt ccagttgggt tttatgtctt
    44521 cagttttgtg agactgtgag atcatcagat aaatattgtc tctgttcatt acacaaaatt
    44581 catgcggatc tccataaaaa ttttgaaaat cgaatccaag tttttcgcgg taccattcgg
    44641 cagatttgtc aatgtcagag acaagtaaaa ttgatgaagt accggttaat ttagctttgt
    44701 ccatatactt ttttataaat tatatttcca ataaaaaaaa ctttgattct ttcaccttta
    44761 tatgctttca atgcaagata gatactgtat gcaattgctc ctaatacaag cagtataata
    44821 aatgcataaa aggcgatgat aagaataata aaaaaagcag gcagctcgga attacctgaa
    44881 gaattaagat ttgacattcc caaccctgaa gcaaaaaata ttacagcaaa gaaaatcaca
    44941 aagaaaataa ttacagcagc tatagagaga tgaaagaaaa ttgattgtaa tgaatgaaat
    45001 cttacgaact ttgatttatc tttttgtgta gcccagatta taagtggaag tagaattcct
    45061 ccaaggatca gagataaatg agaaagcaga gccattgtcc tgtcttcatt tgaaggcata
    45121 ttatcattaa tagttatatt gttatccatg atataattga aattaattaa tatttatttt
    45181 ttagagtatc tcggtatcag atgaatatgt gaatgcatta ctatctggcc tgcatcttta
    45241 ccattgttta taattaaatt gtaagctgaa ggagaatggt ttttatcgga taattttttt
    45301 gcatcttcca tcagatccca caatgaattc cttacatctt cagggagatc aaaataattt
    45361 tcataatgat caaatggtat aacgagtaaa tgacctttgg actgaggaaa tttatcttta
    45421 attacaaatg ctt
//
LOCUS       DAORVQ010000006        43872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_245_length_43872_cov_5.365566, whole genome shotgun sequence.
ACCESSION   DAORVQ010000006 DAORVQ010000000
VERSION     DAORVQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 43872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43872
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_43872_cov_5.365566"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..3220
                     /locus_tag="PLX80_01170"
     CDS             374..3220
                     /locus_tag="PLX80_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19434.1"
                     /translation="MKFYLIFVITIFSVNARSQENINSILDLTLSYRGLDKNDITIPV
                     NFDREKSPANNSKLLLPLVKDLMQTPMNSFEFTDTISRLSNMDINVLFKNLIELKSGI
                     QSSAKFNEGNIKLNSPEDLFNSLVRFCKLKKDQSLKTANKFSKDEKEFLSKNLFSLIS
                     DGESEDEGNTNSDIFKFNQARDSSTAISKKTMDILSSKYSDEVLNNSVNDFNYCNSLF
                     LVILKNFSDFKNVETEFMNNKNLNGDMLFYYDENNIRIAVGGKGNNHYSGHFDLIIDL
                     GGEDTYDIFSSDKKNIFIENFNCIIDLNGNDLYTSKENFNLAGAVFSNSFIFDNEGDD
                     TYRGKNISLGSAIFGAGILCDFSGNDIYQGNKFSIGAGSFGFGILFDVNGNDVYTANS
                     YSQGFGMTEGVGVISDKRGNDSYLIDARSLDIGRYEDHYVSMSQGYGLGLRPYYAGGI
                     GLIIEGEGNDIYNTDIFGQGGAYWYAFGCISDNSGNDKYNSYQYAQGAGIHLAVGILH
                     DKSGWDFYSSNGVSQGCGHDYGVGFLYDENGNDNYSAFSLSQGAGNANGIGIFIDEKG
                     RDGYLNKEPGNSRGYGNSRREYGSLGIFLDASGDDFYSQMSDSVISNTSMWGVMNDFF
                     LKEESYTPSGTNYKVPLDSLKSYSMEEYFVMAKTIEPRFSLWQEFGFRHLVNDSDQTS
                     EYILKYLDTEDHRSGLVLRNLAFKIGYSMGNVFNEKLNLYNNSKQPQTIFNENEVAYI
                     CYLFGETGNASGKDEILGLTYSSNNRIKTVAVNAIGKIKYDTSDNSYKEKVIKRLSEI
                     VMQNNNSKILSKDLAYAFGNYKSENDIPALITLLKSDYFGARFIAASELTSYGDEYFI
                     YLDESNISDLSQKITSFHAFLNSTTNLSLNNFEKLMIKLSSEAIFNDDGIKLYFADIV
                     NKKISSSQLKDEQVSSRLKEMSEEIQMNSPLKIK"
     gene            3243..3896
                     /locus_tag="PLX80_01175"
     CDS             3243..3896
                     /locus_tag="PLX80_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19435.1"
                     /translation="MKNVILKFSFVLIIAVSFSSCVSIDQQTKINKDASGNISLHYWT
                     ATSNLSMGDELGGFSFEEAQVRKKFTSLNSDVTEVKIDNVESDSTSHVRVKITFKDFN
                     KLNEAEGFSKTKTIWKKEKDKIIFNYTLIKDTVSSDKMASDEYKLFYGFSFPDQVLTT
                     NGNVEGNLVTWDKSLSDLNEDIVMSAEINDETGVCGLFGIELPFILLIGLSVFYRRG"
     gene            complement(4089..4736)
                     /locus_tag="PLX80_01180"
     CDS             complement(4089..4736)
                     /locus_tag="PLX80_01180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19436.1"
                     /translation="MKETKFDQWAKNYENDINLLKDQYPFAGYFDIIEVIKKKISEEN
                     LKNILDLGIGSGYMLSKVTDENQKCIGIDFSEKMLEIAGSKFKSKNLIRYDISKGIPD
                     ELKEERFDCILSAYTLHHFNQKEKIEIIKSYSELINKGCFIIADIAFDSIDKMLIVKM
                     DKNKNWDEEEEGGYIIFDSFSECLEENNIKYEYKKMSYCSGIFIIEIQNNKMIKY"
     gene            5340..6527
                     /locus_tag="PLX80_01185"
     CDS             5340..6527
                     /locus_tag="PLX80_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQY19437.1"
                     /translation="MSIFDVLMLKTISPFIFLLFISINTFAYDFSEVDQVISAGIYNQ
                     AYPGAQLLVGNGNEILYSKSYGNFTYDEFSQPVTENSMFDLASVTKVIAATSAIMLLY
                     ENEKIDLNDPVSKYIPEFTGRGKDNITIVDLLLHSSGLIAWIPFYKTCSSKSDVLNTI
                     YDQDLNYSTNSKSVYSDLNAILLGEIVERVSGISLDKYCEANIFKPLGMKNTTYVPAD
                     EIQKNILPTENDTYWRGRLLQGEVHDEAAAVMGGVSGNAGLFSNAGDVYIFMKMMLND
                     GQYYNPYSRGLKAEYMFNPELINLFTSKFTDLGYENSRALGWDTKPDGSNSKYRIPCG
                     ELISENCFGHTGYTGTSVWCDVDRKLIIIFLTNRVYPSRDNNGIRDIRPEVHNKVIEA
                     VSK"
     gene            6564..8120
                     /gene="pruA"
                     /locus_tag="PLX80_01190"
     CDS             6564..8120
                     /gene="pruA"
                     /locus_tag="PLX80_01190"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQY19438.1"
                     /translation="MRLAKFQNEPFTDFSKKKNAKDFEKSLLKVRERFSKEYPLVIGG
                     ENFTSEKKLVSYNPSKRDEILGTFQKATAEMAINAIDLSHNTFQEWKNVPVKKRVKIF
                     IEAAKKMRKRKHDFSAMMVYEVGKNWAEADGDTAEAIDFIEFYAREMLRYAEGQPVVK
                     YPGEKNRLEYIPLGVGVVIPPWNFPLAILVGMTTASIITGNTVVLKPSSDSPAIAQMF
                     VELMEECGLPKGVLTFLTGDGSTIGDLIVTHPMTRYISFTGSMEVGLRINELAAKTQP
                     SQIWIKRVVAEMGGKDSIIVDSEVPDFYEAVKGTVSAAFGFQGQKCSACSRVIVDEKI
                     YDKFCEALVTETEKIKVGDSSLNPNMGPVINEKSKNSIAGYIKKATDEGGKLIHGGAE
                     MSVDGYYIQPTIIKDVERNATIAQEEIFGPVLAVIKSKNFDDAMEIANNTIYGLTGAV
                     FTKNKKKLERAEKEFFVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLGLFM
                     QAKSIAQKIK"
     gene            8180..8464
                     /locus_tag="PLX80_01195"
     CDS             8180..8464
                     /locus_tag="PLX80_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19439.1"
                     /translation="MTKISVSLIFLFLAVTVYACGIRENIENRVNEKINETVDKSLLK
                     IDSALDNTKTMLDTMGNKTFEGMDSVKYQLDSVSKEIKEMIEKQEKLLKK"
     gene            8507..9721
                     /gene="sucC"
                     /locus_tag="PLX80_01200"
     CDS             8507..9721
                     /gene="sucC"
                     /locus_tag="PLX80_01200"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQY19440.1"
                     /translation="MNLHEYQAKSILKKLGINVQEGVALMDMQYFDEAIEYLKSKDIK
                     KYVVKSQIHAGGRGKGVVYRKDKPDEIVVEGGVKYMGNDLQKAKEYAGKLLGNVLVTH
                     QTGKEGKQINTIFIAEGLDIKKEFYIGILLDRSVSRNVIMASTEGGVEIETVAEETPE
                     KIIKVWVDPSVGLLSYQARELALGLGLEGNVYKDFIKFITKLYNAYVTMDASLLEVNP
                     MVITNDDKIVALDAKVTLDGNADFRHKDYKELEDTNEEEPLETEAKKHNLNYIKLDGN
                     VGCMVNGAGLAMGTMDLIKLAGGSPANFLDVGGTATPDTVENGFRIILSDPNVKAILI
                     NIFGGIVRCDRVANGVIQAAKNIDITVPVVVRLQGTNAVEAKEILDNSGLNLISATTL
                     EEAAKKVTEALN"
     gene            9789..10859
                     /locus_tag="PLX80_01205"
     CDS             9789..10859
                     /locus_tag="PLX80_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HQY19441.1"
                     /translation="MEKINISKNGSRTYSSKISETYSFTYDDISLLPDQISEIEHRAE
                     CDTSVDFLGINLSLPIIGSPMNTVVGGRMAKALADLGCLGIVHRFNTIVEQIKEFTDN
                     DMADSDFKCPAIGINKDTDLVRLKSFADIGVKVVCIDIANGGSKMIEEFISSASLTIS
                     DYKMKIIAGNVASYETTEFLINIGVDAIRVGIGNGAMCSTSVKSGIGIGQVDAILRSL
                     EAKNDLKSDVKIIADGGISTPGAMCKAIALGCDAVMFGRVLSGTEESPGDVLKYNNQR
                     WKKYCGSASFAVKQDKKNYIEGEESLVPYKGLVSKIITEYKEGLQSSMSYLNSRTIKE
                     FQEKAAFVKLTNSSFNERKPQI"
     gene            10859..12304
                     /locus_tag="PLX80_01210"
     CDS             10859..12304
                     /locus_tag="PLX80_01210"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQY19442.1"
                     /translation="MHYIFWFRKDLRTEDNKGVSEFTKVVSAGHKFSLIYIKNKNSFK
                     YFGEKRISFLHNSLAELSDVIGTKKLKLQILYGNSTEIFRFLTEKYGSISLYYNIQVE
                     PYCIKRDSDVSEIIGKSGGNVNEFTDSTIFTPGEIQNLSGDQYKVFTPFKNYVLGRLG
                     DRHFAKIKTDFSSLDISNELIIAQNSVKKVQSFENKKLQQGILKGGRKEGLVLLKKFY
                     ENGIKYYKKNRDYPAVKGTSYLSAHLHFGTVGIREALRTAKAALKNAKDESSAIGVQT
                     WINELLWREFYYHMTFFNPDLTFRSFKKECDDLNWSYDENDFEKWCSGQTGYPIVDAG
                     MRQLNNEGWMHNRLRMITAMFLTKDLFIDWRLGEKYFADNLIDMDFASNNGGWQWCAS
                     TGADAQPYFRIFNPYLQSKRFDTNGDYIRKYVPELINVPLKNIHEPHLMSHDDQKKCN
                     TIIGKDYPLPMVKHSKVKKRVLSEFKKLNKK"
     gene            12320..13813
                     /gene="gatB"
                     /locus_tag="PLX80_01215"
     CDS             12320..13813
                     /gene="gatB"
                     /locus_tag="PLX80_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQY19443.1"
                     /translation="MESEINKYNRYEPVIGLEIHAQMKTNSKAFCRCSTNFHSSPNSN
                     VCPVCLGYPGALPVLNAELVDYIVRMGIATNCSIREVSIFARKNYFYPDLSKGYQITQ
                     FDTPICYDGHLDITLKNKEEKKIGITRIHMEEDAGKSIHDFSDNDSLIDFNRCGVPLI
                     EIVSEPDIRSTEEAFLYLSKIKQILMYLEINDGNMEEGSLRCDANISVRLNGAEEFGT
                     RTEIKNLNSFRNVSDAIEAEIKRQIDLLESGEKVIYNTMTFNAKDKTTAATRSKEEAH
                     DYRYFPEPDLVKVRMDEEWIAKINSALPEFPDARRDRYIKDFSLPYSDADEITQDKYF
                     ADYFENAVDNLSDKESSSYKNVSNLVRSDVRRILNEEKINIDKFTVPEKIIAMIAESV
                     TSGNVSATASKEIFNAVLKGNSEDEMLSLFKKAESDFSQVSDSSELESIVKKILEDNP
                     KEVDRYKRGEKKLAGLFVGKIMQESKGKANPKIVNELLIKFLEIETE"
     gene            13820..14590
                     /gene="tpiA"
                     /locus_tag="PLX80_01220"
     CDS             13820..14590
                     /gene="tpiA"
                     /locus_tag="PLX80_01220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQY19444.1"
                     /translation="MRRKLIAGNWKMNKSYNESQILAEEISKNIEPTITENTDVLICP
                     PFISLGVISEIIKDSGIKLGAQNVYSENDGAYTGEISAEMLKSVGCEYVIIGHSERRK
                     FFYETDEDVNAKVKKALDNGLKVIVCVGETLEEREKGDFEKAVTAQVTGALEEISKES
                     MRDVVIAYEPVWAIGTGLNATPEQASEIHGMIGNTVSKLYDSDTAENLRILYGGSVNA
                     KNAKEMLSACGVDGALIGGASLKPDDFISIANEASKIK"
     gene            14622..15797
                     /locus_tag="PLX80_01225"
     CDS             14622..15797
                     /locus_tag="PLX80_01225"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQY19445.1"
                     /translation="MIETVIVAAVRTPVGSYMGSLSSLKGPELGAIAIKEAIIKANIK
                     PEDVSEVIMGCVLPAGMGQAPARQAAIFAGIPDSVPALTINKVCGSGLKSVMLADQAI
                     KCGDAEIVIAGGFESMSNVPYYLDKAREGYRMGNGTLIDGMVHDGLWDVYNNFHMGSA
                     AEMCAGEMNISREEQDSFAEESYNRAIKATKDGLFKDEIIPVEIKGKKGSVIVSEDED
                     PTKVNFEKGKTLRGAFDKDGTVTAFNSSSLNDGASALVLMSADKAKELGLKPLAKIVA
                     QASFAQKPEWFTTSPAYAIKKVLEKAKLEVKDLDLIEANEAFSVQAFAVNKIAGLDSA
                     KVNVNGGAVALGHPIGASGARILTTLVHAMNNRDAKRGLATLCIGGGEASALIVEKV"
     gene            15838..16689
                     /locus_tag="PLX80_01230"
     CDS             15838..16689
                     /locus_tag="PLX80_01230"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HQY19446.1"
                     /translation="MNNIAVIGSGTMGNGIAHTFALFGYKVKLIDIKQEFLDKSIATI
                     TKNLDRQVSKGSLTEDQKVSALKNIVTTVNMSDASDSDLVVEAATENIEIKKKIFSEL
                     DKCCGADAVLASNTSSISITEIGAFTNRPDKVIGMHFMNPVPVMKLVEIIRGLATSQE
                     TFDKIKTLTEKIEKTPVEVQDYPGFVANRILLPMLNEAMYCVMEGVATPEAIDNVMKL
                     GMNHPMGPLTLADFIGLDVCLSIMNVLHDGLGDSKYRPCPLLKKMVAAGYLGRKNGRG
                     FYHYSQK"
     gene            16719..17861
                     /locus_tag="PLX80_01235"
     CDS             16719..17861
                     /locus_tag="PLX80_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY19447.1"
                     /translation="MNFDLTEEQISIRDAAKDFAENEIAPSAVERDLNAEFPAEIVKK
                     LGELGFMGMMASPEWGGACMDTVSYVLAIEEISKVDASVGVIMSVNNSLVCYGLEKWG
                     SDFLKEKYLKPLASGQKLGAFCLSEPEAGSDATQQKTEAVLEGDHYIINGIKNWITNG
                     VYCDFYFLQAMTDKSKGYKGISTFIIDKDTPGLEVGKKEDKLGIRSSDTCTLGLNNVK
                     VPKENLIGDEGIGFKIAMETLNGGRIGVAAQALGIAQASLEASVKYSKQRKAFDSTIS
                     ELQSIQFKLSEMATSIEAARLMIYSAAINKDSGKSYIKEASMGKLLASQAAVNCALEA
                     IQIHGGYGYVREYLVERYLRDSKITQIYEGTSEIQKLVIARELLKN"
     gene            complement(17851..18561)
                     /locus_tag="PLX80_01240"
     CDS             complement(17851..18561)
                     /locus_tag="PLX80_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19448.1"
                     /translation="MKKYILTALILFISSNSFSQIKSRSDNINYYAGIGYKLVFLTDN
                     TARNAFPFFQLSSGDFMKEITGQFGAVFSETIAAEFAPAYMFTTSYSTDGFYYTGAVN
                     GSRFYVPTDSRLFALPLNLKVKVFPQGKNYTSFLNKVYISAGAGMMYFSEEIIAQVYT
                     DDINYNYIGSARAEDEFWNFNYEFAIGLASYSKIGYGFELSYRVVPLDNDVNKPLITT
                     ISSNFNSVNLSANIIFSF"
     gene            complement(18580..19965)
                     /gene="hslU"
                     /locus_tag="PLX80_01245"
     CDS             complement(18580..19965)
                     /gene="hslU"
                     /locus_tag="PLX80_01245"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQY19449.1"
                     /translation="MKQEIRQLTPSEIVEELDKYIIGQQNAKKSVAIALRNRWRRQQV
                     GDKMKDEIMPNNIIMIGPTGVGKTEIARRISKLANAPFIKVEASKFTEVGYVGRDVES
                     MIRELTDLSVNIVKTHKMEEVEIKAMENAEERILDILLPPMKKSANGTDDEAASKDEK
                     AEAAELNARTRVKLREQLRKGALDSRMIELDLSSDNFPMLQVLGPIGLEEMGINLNDM
                     FGNMMPKKSKKRKLSIAEARKVLSQEESGKLIDMDAVIKEATDKVQNSGIVFIDEIDK
                     VAGSNAKSQSPDVSREGVQRDLLPIVEGSTVITKYGPVVTDHILFIASGAFHSSKPSD
                     LIPELQGRFPIRVELNSLGEDEFYKILTQPENALIKQYMAMFDTEGVKLTFQDEAIKE
                     IARIAAQVNEQVENIGARRLHTILTTVLEDLLFEIPDKFKTKKIEITKEYVCKKLDLI
                     VKDRDLSRYIL"
     gene            complement(20056..20598)
                     /gene="hslV"
                     /locus_tag="PLX80_01250"
     CDS             complement(20056..20598)
                     /gene="hslV"
                     /locus_tag="PLX80_01250"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQY19450.1"
                     /translation="MKNKNRIRSTTVIGLIKDGKAVIGSDGQVTMGNTVMKHKTKKVR
                     KLYNGKVLAGFAGSTSDAFTLFEKFESKLDQYKGNLSRAAVELAKEWRSDKYLRRLEA
                     LLAIIDSEKALIISGTGDIIEPDDGIVAIGSGGQFALVSARMLLKYSKLSAKEIVEES
                     LKQAADICIYTNSNISIEEL"
     gene            complement(20614..22101)
                     /gene="rpoN"
                     /locus_tag="PLX80_01255"
     CDS             complement(20614..22101)
                     /gene="rpoN"
                     /locus_tag="PLX80_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQY19451.1"
                     /translation="MLNQNLSQKLSQKLLPKIVLNQNILAIPTLALDTIIKRELELNP
                     MLEEGPELDQDIEKSAEQNSETTEIKGEETTEENYDKDDEYNWDEYFENESDNEPYYT
                     SGGAGSENEFDRNNIREEEQSMYDNLIVQLHLFDLSKREIFIGEEIIWSLDNDGYFKE
                     SLEAVLEDTDVKKRGTEFENDNFSEEDILNMLEYIQVNIDPPGIAARDLKECVIIQVK
                     RSNINDRIKENTIKIFTEYFDDMKHKRFEKLSKELDINMEEVKEIFEFVHTLNPKPYS
                     QDTDANVGYIVPDLIVKKTEDKYEVYLNEKFTPSLRISRTYSDMYTKDKKNLDSNTKE
                     YLLNNFNKAKWFIDAINSRRETMIKIMEAIINRQKEFFENNGENLNPVFEKDIAEDIR
                     MDNSTISRAVKGKYVQTDFGIYNMRSFFSNSIPNKDGSDISTIEVRNKIKEIIEKEDK
                     SNPLTDQEISEILTKSGLKIARRTVAKYRESVDLPIAKQRREIIN"
     gene            complement(22101..22649)
                     /locus_tag="PLX80_01260"
     CDS             complement(22101..22649)
                     /locus_tag="PLX80_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HQY19452.1"
                     /translation="MEKFKIDDRIFTTDFACNISKCKGACCTLKGAGGAPLLEKEINE
                     IKSSLKSVKKYLCEEKNRIIDQDGFTNGTGDDLELKSMDDEDCVFVFREEGIAKCAFE
                     RAYFNDEIKFRKPISCQLFPIRISGKKRNTLRYEEIYECRDALDEGKEKNIKMIDFLK
                     ESLTREYGKEFYKNLKEKFGYK"
     gene            complement(22660..23778)
                     /gene="dprA"
                     /locus_tag="PLX80_01265"
     CDS             complement(22660..23778)
                     /gene="dprA"
                     /locus_tag="PLX80_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQY19453.1"
                     /translation="MISLKYIYFLTKVKKLGNNRIKKILKAVPDEDDFFNCSRNDLKL
                     IDGIDSSVSAEVIRETEKRSFYDDEFEKEFDRSIKKGIRIVSILDNDYPENLKMIYDA
                     PALLYIKGKTEKSDRYSLSIVGTRNPTEYGKYTCEKFAQQLSEFGIPVISGFARGIDS
                     IAHRECIKQGNLTYAVLGCGIDVIYPPENRKLYDEIINNGVFISEFSPGSIPDKMNFP
                     KRNRIISGISIGSIIIESGIKGGSLLTADFANDQNREVFAVPGYINSRQSDGTNELIK
                     KGQAKLVTCIDDVLIELEFKLKRFLNKEKEISDNKKEMVLSSTEKKLYDLIGFEMKNI
                     DRLNEESGCPVSDCLVSLLTLEFKGLIRQLPGKNFMRL"
     gene            complement(24081..26681)
                     /locus_tag="PLX80_01270"
     CDS             complement(24081..26681)
                     /locus_tag="PLX80_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19454.1"
                     /translation="MKKVITDLKKGRYFLHFSLLVICTVIFFAFNSAEDAVISSNNMS
                     PEWNAFHTVSLQDDYTLGIPVQGEPGITVTVQEIMDREAMLPADIYLKPKPKTNPEFE
                     IEMPEKQVNPNAPDVSQWPVLSEDEVKKEIQQTDNPQTIGTNFQGPKLSESGYLPPDT
                     QGDVGPTQVLIAANGRIKVYNKAGVVGGLNSDLDVFFNSVRNGAGISDPHVRYDRLTQ
                     RWYVVAINTQAGPNRVVIARSSGPTITNLASFTFFQFTANLPGSLSGFADYPTLGVDN
                     NALYIGMNMFNNNVTAYLGTNGYVVNKSNLNAGSLTVTAFTLFSNGGASGPQTPQGVQ
                     NDDPNSTEGYFVGVDPYVYSVLSVKRVINPGATPSVSGNYAITVPSTGAPETAPQTGS
                     TKRLDALDDRLFAAELHKNKITGSQTLWTAHNIEVNSSGVASSTGSRDGCRWYELTNM
                     TSTPALLQSGTLFDPAGSNPRYYIIPSVAMSGQGHMALGCSSAGLANRPEVQVAGRYR
                     TDAAGTIQSPILAQSSTSVYGVQGGTTQRWGDYSQTVVDPNDDMTMWTFQEYCDATNS
                     WSVRAIQLIAPPPATPSASNVTDIMQGLPSVNIVITGNSISNSEFFDPGNDAGGPGYA
                     NRIAASISGTVTVNSITFNSPTQVTLNISTVGSPTGLKNITVTNPDGQNRTGNNLINV
                     TAPATLNLTAYIQGFYNAGSNTMVRDTMTVFLRNNTAPYAVVDQAKVYLSNTGTGVYV
                     FNNAVNGVNYYLQLKQRNLIETWSKSPGQSFTASVLNYDFTTASAQAYGSNQIIADNS
                     PVKYAAYNADANQDGIVDLADGGMIDNDAFSFITGYVVTDADGDGLVDLADATLADNN
                     TNNFVTKSIP"
     gene            complement(26974..29442)
                     /locus_tag="PLX80_01275"
     CDS             complement(26974..29442)
                     /locus_tag="PLX80_01275"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY19455.1"
                     /translation="MIRKITFTFVMILMVSGTLFSQDKQDWKWMHPSTQSNLLRKIKM
                     VDENTWVTAGANGTYMQTTNSGNNWYFHHFAGEADANLRTTQNYDMWFFNQNTGIVVG
                     DQGYIGRTVNGGVSFDTVSAGLIETNKRNWAVWFADANTGYIASGSQNAFTTKLLKTT
                     NAGLTWAIVFSSNTNYAVSIGGSDANNVTASYSNGVTITTANGGTTWTESAGVVLFQI
                     PYNIEFLNSNTGFACGTGGQFSRTTNGGANWQSIATPQIDWAYFQIKIVSETEIYLVG
                     DPSFLYRSSDQGATWTTLSLSVSGPATTFIWYSMDHVGSTYVLSGDYGVVAKSTDGCA
                     TWNSESDLLKTALNFNITTIPGTSKYWIIGRPHTATTGQIMYSSNSGTNWTTYGIGVT
                     GDFNDISMINENTGYVTGQNSQVYKTTDGGVSWVMKTKPHPTNYTLYNCDFIDENTGW
                     VFVNFATVSGGNVFKTTDGANTWTQYTTGASSENIYSAEMLNANTGYTTMNQSNRPVY
                     KTTNGGVNWSPLTTGLTGSIRSVTCPDDTTVYICQTSGTNRVAKSTNSGATWTLISMP
                     ATGDFTSIDFKDANTGYACGNSTTVVCRTTNGGVNWTFQNTHTITQGKVFVTQGDTAW
                     VLGGNAAVMRYTGNNSQLKLNLTVLMEGMYSPGTNDLSLSDDVKVYLRNSTAPYAIVD
                     SATAVIDSLSFTGSFTFPNAQSGQYYIVAKYRNTIETWSKAGGELILDKGTINTYNFT
                     TAAAQAFGSNQILIGSKYCNFSGDINNDGTVDLGDLGSIDNDAANFVTGYVTNDLNGD
                     DAVDLSDISIGDNNANNFVQKITP"
     assembly_gap    29821..29920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29983..31386)
                     /locus_tag="PLX80_01280"
     CDS             complement(29983..31386)
                     /locus_tag="PLX80_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HQY19456.1"
                     /translation="MRISIENQLIGKIGSIADTNKVTAYLVGGYVRDLIMEIDGNDID
                     IMVTGDGISFARTVAKEFGRELNSVYKNFETALLNVDEYKIEFVSARKESYDRGSRKP
                     LVETASLEEDISRRDFTINALAAGINEKNFGEVIDNFHGLKDIENKIIRTPLDPYKTF
                     DDDPLRIMRAVRFASRFDFKIEENTFNAIREMRHRLSDEGVVSQERITHEFLLILMTD
                     KPSIGLDLLYRTGVMEIIFPEIAALEGVDQRKDFHHKNVFYHTLKVIDNIVPNTNNVW
                     LRFAALVHDIAKPRTKKFVEGTGWTFHSHEEVGARMMKKLFVRLKLPMTKLDYVEKLV
                     RMHLRPIPLAKDDVTDSAIRRLAADAGEELVDLLTLCRADITSKNPDKVSKFLDNYTK
                     VEKRVVEVQEKDELRNFQSPVRGDEIMKLFGLEPGRIIGVFKHKIEEAILEGEIPNNY
                     DAAKEYLLKIKEDVLNS"
     gene            complement(31386..32255)
                     /locus_tag="PLX80_01285"
     CDS             complement(31386..32255)
                     /locus_tag="PLX80_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY19457.1"
                     /translation="MFKIAHISDTHLRLDDINGGGKAFVELMEDIKKKNCDHVILSGD
                     LVDNVNEKDFRFAKELLKNYDLVRGDKFTLVPGNHDIFGGAEMGIEGLKFIFKCSNAD
                     YDLNVKIFAEHFNESFPETIEFPYLKIFDNVAIIALNSIDRWDIKKNPEGSNGRIDKM
                     EFENLEKLLSIKKIKKMIKIVVLHHHFGLPESTEENPAHPVWLAGIDVKMKLYGKKKL
                     LKLLEKYKVKMILHGHTHISEIYTKGNIVIINSSASAIPLTDDRVRSYHIIEIPGKKD
                     EEREIKIEKVIIP"
     gene            complement(32286..33281)
                     /locus_tag="PLX80_01290"
     CDS             complement(32286..33281)
                     /locus_tag="PLX80_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY19458.1"
                     /translation="MHKIKAGLFGLGHLGKTHLKLIKEICNEREDVELAGIYDTDTEK
                     NKMLSDENNLDVNSSAEELLKKINTAVIVTPTSTHFELASKCIEQNINTFIEKPVTET
                     TKQAVELLKLADNKDIKIQIGHIERFNPAILSLDSYNISPLFIETHRMSQFNPRGTDV
                     SVVQDLMIHDIDIILNLVKSPVESIHANGASILSEKIDIANARLQFKNGCVANITASR
                     ISLNKMRKMRLFQKNAYLSIDFLQNTSEVFRLSDNKDDTGFAVTEVEQGDKKLKIFYE
                     KPEIPEVNSLKYELNRFFDSIIKDEEPLVTLTDGLQALEVANKIIEEIEIAQIKK"
     gene            complement(33327..34751)
                     /locus_tag="PLX80_01295"
     CDS             complement(33327..34751)
                     /locus_tag="PLX80_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HQY19459.1"
                     /translation="MKESSKTVSDKKIKVAVQSLYTAVFITLIKIVAAFFSGSLGILT
                     EVFNSGIDIFVCIVTILSIKYSSRPPDKDHNYGHEKAENFSALFQVFILFITSSVIIY
                     EAINRLFIKEEVELNINIWIIGSIIFTILIDINRAKKLKAIAKETNSSALEADALHFS
                     SDIWSSSVVLIGLLLTYLNISRIADTIAALVVTIFIIHLGYKLLKKSINLLMDKVPEG
                     LYEKIKYETLLVKGVEGINSCRIRTSGSITYIDMTIEISRLVPFSKAHDIMDNVEKRI
                     KEISENTDIVIHSEPVETKDETINDKIRIIVNDFGLKCHDIYSHRINNEIFSELHIEV
                     DNTNDLTKAHSIITDVEKEILEKIDVISQLKIHIDEPSEIVFDTTDITDNSTELIREV
                     NRIIDENENVLSSNSIQVVSTNGKIRVSLNCVFDYINSFDEVHDFVTQLESKIYLSLK
                     ENYPKLSNVIIHSEPSETSSDNFK"
     gene            complement(34735..35661)
                     /locus_tag="PLX80_01300"
     CDS             complement(34735..35661)
                     /locus_tag="PLX80_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY19460.1"
                     /translation="MKKFILVFFLTFTAGFTPIAAKYVVGAISPLSLAFLRFGMATLL
                     LLIVFYYKKGKFKIDKKDWLRFFILGMLVIPINQFCFLIGINLSEASHSGILYACTPL
                     IVYLISIKQKDEKFEMKKLITISLTIVGILVIFYENLITPKIPGVNYMLGDSLLFLAV
                     ASWSMYLAFSQPMIKKYGALKTMTISFVIGIVMYIPVFLYDVNNLTFENVNIYVILGY
                     IHLTFLVAFGSYFLYSYSTKLIKTSTLTTMTNTAPVITILFSWLLLNEKLSYFFVAGA
                     IITMTGVVLTQRLKDENLIKNLERPYINERKQ"
     gene            complement(35979..37043)
                     /locus_tag="PLX80_01305"
     CDS             complement(35979..37043)
                     /locus_tag="PLX80_01305"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HQY19461.1"
                     /translation="MNKKTLKILGKENEYLLSHKCKTISKDMLHLPGSDFIDRVWKDS
                     DRSPNVLRNMQSIYNSGRLAKTGYVSILPVDQGIEHSAGASFAPNPIYFDPENIVKLA
                     IEGGCNAVASTLGVLGSVSRKFAHKIPFILKINHNEFLSYPNKYDQIMFADIDQAFDM
                     GAVAVGATIYFGSDESATQLQEVAQAFSYAHELGLVTILWCYLRNSAFNVKGDKDYHL
                     SADLTGQANHLGVTIEADIIKQKLPENNGGYNALNFGKTHKFVYDKLSSDNPIDLTRY
                     QIANCYMGRCGLINSGGASTGKGDSDLKEAVKTAVINKRAGGMGLISGRKAFQKPMKD
                     GIELLNTIQDVYSDDSITIA"
     gene            complement(37170..38099)
                     /locus_tag="PLX80_01310"
     CDS             complement(37170..38099)
                     /locus_tag="PLX80_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19462.1"
                     /translation="MPVSYFINKSGLIKASGTDLTDFINRMSTNDFRSFPEGECRYTV
                     LTSDKGRIIDLVNFVNFGEEKYLICTSGFEEKIIAHLDKYIIMDDVKLEIARGDYSRI
                     TIFTDEYESLSSDLFNIKCEKDKAYRINKSDILFLSDGKIKTLNIICEEKDVSVYRNL
                     MKDLTALTFDEYEFKRISNLIPEGENEFNDQINPMECGLEKYISFKKGCYIGQEVIAR
                     IDSQGKIPKSMVSIVSDKEIIRNDKVINSNSDRETGFISSVIKNDDKYYGLAFLRRTE
                     LVEENKFYVLRDGNKNEINILKKEIESETKSFM"
     gene            38649..39971
                     /locus_tag="PLX80_01315"
     CDS             38649..39971
                     /locus_tag="PLX80_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19463.1"
                     /translation="MIIIAVIIVICLFVYKEFNKDKVVIEVFQVPMDLEKQNITGQAV
                     VNKLLDNIIKIESQADTSFPLLDFNPVMYDAQMEIVIPGSGISINSLIRHVKNFLGKK
                     QIKITGEIVVSDKKLLMTTRIVGEPAMTYTGDLNSIDSLIYESAKHIILYTQPYMLAY
                     YYYYSYGSDHLNKELAMEMINYSITQPPVTDDVMAYTLYGYILSDQGKYEESINMYEN
                     ALEIDPDFTDAISGWAYSLFEMNKFEESEDLYKKALRTDPNDSYTYYYYALLKEKMNS
                     YSQADSLYKKAVSLDPKNFEAANDYSNFLILQNRLNEARVSIKNILKVRPDNSQAYFN
                     LGNLNYNENNYEESVNNFLKSFSLDSTNVAALLNASRILNENDRNEEAIDLVKNIDLK
                     KNYDFQTTKDIGILLENLEKKSESIEFYKRSIKQFPNDSAYFRNRMVN"
     gene            40375..40779
                     /locus_tag="PLX80_01320"
     CDS             40375..40779
                     /locus_tag="PLX80_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19464.1"
                     /translation="MKSIYLALSLFLALFISSCSSDSSSGNNPFVPGGGTGTTVTFTT
                     TYDANNTQFVFAPNTGVTISKITANCAAANINNEEITGDGTTVWTQAAPAAVSTQGVN
                     VAAGQQWTFKIEGKVGSATGEVYTSNTSITVQ"
     gene            40848..41507
                     /locus_tag="PLX80_01325"
     CDS             40848..41507
                     /locus_tag="PLX80_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQY19465.1"
                     /translation="MKKLNNIALLIIVLLIVFASNNSNAQVKIALGLQAGVNFGNASN
                     NVAGVDQTLSSSTGFIAGGLVDIGLSKQISIVPGLRYIGKGSKQTNVAVQGLGNGDLK
                     TTIGMLEFPALLRVKFPLTEVNPYLIAGPILGIILSANTDFIPTAGGTTQSFDVKSTF
                     ESTEFDLYFGGGLDFKISPSASLFFELGYELGLTDIAKASNVTTKSYSFQITGGARFG
                     M"
     gene            41655..42323
                     /locus_tag="PLX80_01330"
     CDS             41655..42323
                     /locus_tag="PLX80_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19466.1"
                     /translation="MSAKHHQEDKLMSAYYKAVDFFEKNKKHVYTALTILIVLIAGTV
                     LFVNKRTANNELAAVEIEKVKPLYNAGNFEQAVNGDSLGNSKGLIYIVNEFGSTENGE
                     YAKLMLANSYYSLRDFDNAEKYYKEFSGSNDVLKVSAIAGLAGVYEAKNDYLNAARQF
                     QKAANTDKENPFVDQYLFYAAKDYFRAGNFDESKKLLDELKKDYPKSKYIAESERFRA
                     SMKN"
     gene            42373..42819
                     /locus_tag="PLX80_01335"
     CDS             42373..42819
                     /locus_tag="PLX80_01335"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQY19467.1"
                     /translation="MSLKNKINEDLNTALKAKETVRIETLRSIRAEILKMDKSGMNRE
                     MNEDEEIQLLSKQAKMRKESIDLFREAARIDLVDKEQAQLDIINEYLPKQMSADEAGI
                     IVDNILKESGITEQKDFGKLMGEVMKQLKGKIDGKIIQDLVKSKLN"
     gene            42923..43450
                     /locus_tag="PLX80_01340"
     CDS             42923..43450
                     /locus_tag="PLX80_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQY19468.1"
                     /translation="MNTLDLIISITILILSLLGLQKGFLKSVFSLIGIVAGFLLATNY
                     NEVLTGYLSFIKINERILSLISFLLIVVIINIILNYVSQKISGFNFATKTIDKLFGFL
                     FGMFKGIILTSLFLLATTKLFSAFSKKTIDDSKIYPYVIDAAPGIYNNIMTVLPNAKN
                     FYQEFDFLKDFNNKK"
BASE COUNT        14375 a         7386 c         7429 g        14582 t
ORIGIN      
        1 ctgcctcaat gtctctaagg cattaagtta ataaaaataa aatacagtta aagcagattc
       61 acgagctttt cggactctgc tatatggaaa atgctgaagg aaaagactat aagactctaa
      121 gactctaaga ctctaagaat ctaagtttaa aaaaaatata ataccattta agcagattca
      181 cgtgcttttc ggactctgca catatggaaa atgctgaagg aaatgactca aagactctaa
      241 gactctaaga atctaagact aaatgtttag aaatattaat aaaaaatttc ttatagtctt
      301 cgagactttg aggctaactt ctgaatattt ataaaatttt taaaaaatta tattacacct
      361 actaaattct cagatgaaat tttatctaat atttgtaatt acaatatttt ccgtaaatgc
      421 ccgatcacag gaaaatataa attccattct cgatcttaca ttatcttaca gaggtctgga
      481 taaaaatgac attacgatcc ctgtaaattt cgacagggaa aagtctccag ccaataattc
      541 aaaactattg ctgcctttag ttaaagacct gatgcaaact ccgatgaatt cttttgaatt
      601 taccgatact ataagcagac tgagtaatat ggatattaat gttcttttta aaaatctaat
      661 tgaactaaaa tcaggcattc agtcttcagc taagttcaat gaaggaaata ttaaattgaa
      721 ttcacctgaa gatcttttta actctttagt cagattttgt aaattgaaaa aggatcaaag
      781 tttaaaaact gcaaataaat tttcaaaaga tgaaaaggaa tttctatcaa aaaatctttt
      841 ttcactgatc tctgacggag agtctgaaga tgagggaaat acaaattccg atatcttcaa
      901 attcaatcag gctagagatt cgtctactgc tatttcaaaa aaaacaatgg atatactttc
      961 ttcaaaatat tctgatgaag ttttaaataa ttctgtaaat gatttcaatt attgcaattc
     1021 tctgttttta gttattctaa aaaacttttc tgattttaaa aatgtcgaaa ctgaatttat
     1081 gaacaataaa aatttgaacg gtgatatgct tttttattat gatgaaaata atattcgaat
     1141 tgcggtcggc ggcaagggaa ataatcatta cagcggacac tttgatctta tcatcgatct
     1201 tggaggcgaa gatacttatg acatttttag ttctgataaa aaaaatatat tcattgaaaa
     1261 tttcaactgt atcattgacc ttaatggaaa tgacctttac acttcaaaag aaaatttcaa
     1321 tttggcagga gcggtatttt caaatagttt tatttttgat aatgaaggtg atgatacata
     1381 ccgtggaaaa aatatctcac tcggatctgc gatatttggt gcaggaatat tgtgtgactt
     1441 tagcgggaat gatatttatc agggaaataa attttctatc ggcgcgggtt ctttcggatt
     1501 tggaatttta tttgatgtga atggtaacga tgtttataca gcaaactcat attcacaagg
     1561 attcggaatg acggaaggtg ttggagtaat atctgataaa agaggaaatg attcttacct
     1621 tatagatgcg cgctcgcttg acatcggcag atatgaagat cattacgtct cgatgagtca
     1681 gggatacgga cttggactga gaccttatta tgccggtggt attggattga taatcgaagg
     1741 tgaaggaaat gatatttaca atactgatat tttcggacag ggcggagctt actggtatgc
     1801 gtttggatgt atttcagata attcgggaaa tgacaaatat aattcttatc aatatgcgca
     1861 aggtgcaggt attcatttgg cagtcggaat tttgcatgac aaaagcggat gggattttta
     1921 ttcttcaaat ggcgtctcgc agggatgcgg tcatgattat ggagttggat ttttgtatga
     1981 tgaaaatggc aatgataatt attcagcttt ctctctttca caaggcgcag gtaatgcaaa
     2041 cggcatcggt atttttattg atgaaaaagg ccgggacgga tatctgaata aggaacccgg
     2101 taattccaga ggctatggaa attcacgcag agaatacggc agtcttggaa ttttccttga
     2161 cgcatcgggt gatgattttt attcgcaaat gtcagacagc gtaatttcga atacttcaat
     2221 gtggggcgtg atgaatgatt tttttttaaa agaagaaagc tatacaccga gcggaacaaa
     2281 ttacaaagtt cctttggatt ctcttaaaag ttattcgatg gaagaatatt ttgtaatggc
     2341 aaaaactatt gagccgagat tttcgctgtg gcaggaattt ggattcagac atcttgtaaa
     2401 tgacagcgat caaacttctg aatatatttt aaaatatctg gatacagaag atcacaggtc
     2461 aggacttgta ctaaggaatc ttgcatttaa gatcggatat tccatgggaa atgtattcaa
     2521 tgaaaaatta aatttatata ataattcaaa gcagccacag acaatattta acgaaaatga
     2581 agttgcatat atttgttact tatttggtga gacaggaaat gcttcaggta aagatgaaat
     2641 tctcggactt acttacagca gtaataacag aataaagact gttgcagtaa atgccatcgg
     2701 aaaaatcaaa tatgatactt ctgataattc atataaagaa aaagtaatta aaagactttc
     2761 agaaatcgta atgcaaaata acaacagtaa aattttatca aaagacctgg cgtatgcttt
     2821 tggaaattat aaaagtgaaa atgatatacc cgctttgatc acacttttaa agagtgacta
     2881 tttcggtgca agatttattg cagcaagtga acttacttct tacggagatg aatattttat
     2941 ttatctagat gaatctaata tttctgacct ttcacaaaag attacttcat ttcatgcatt
     3001 tttaaattcc actacaaatc tatctctgaa taattttgaa aagctgatga taaaattatc
     3061 atctgaagca atctttaatg acgacgggat aaaactttat tttgctgaca ttgtgaataa
     3121 aaaaatttcg tcttctcaat taaaagatga acaagtcagc agtcgattga aagaaatgtc
     3181 tgaagagata caaatgaatt ctccattaaa aattaaataa ctataaatta atatttataa
     3241 aaatgaaaaa tgtaattttg aaattctcat ttgttttaat tattgctgtt tcattcagct
     3301 catgcgtgag tattgatcag cagactaaaa tcaataaaga cgcttcagga aacattagtc
     3361 tgcattactg gactgcgact tcaaatttat caatgggcga tgaacttggc ggattcagtt
     3421 ttgaagaagc acaggtaaga aaaaaattca catccctgaa cagtgatgta accgaagtaa
     3481 agatcgacaa tgtcgaatca gattcgacaa gtcatgtaag agtaaagatc acatttaaag
     3541 atttcaataa attaaatgaa gctgaaggat tttccaaaac aaaaaccatc tggaagaaag
     3601 aaaaggataa aataattttc aattatactt taataaaaga tactgtgagt tcggataaaa
     3661 tggcatcgga tgaatataaa ttattttatg gatttagttt tcctgaccag gtattgacta
     3721 cgaacggcaa tgttgaaggc aatcttgtaa cctgggataa atctctgtca gatcttaatg
     3781 aagacattgt gatgtcagca gagataaatg atgaaacagg tgtgtgcgga ttgtttggga
     3841 tagaactacc ttttatatta ttaattgggt tgagtgtttt ttacagacgg gggtgagttg
     3901 gtttcataat ttttccgtat cagttatatc tcatttccgc aacagccccc ttttctaagg
     3961 gggaattcac ttataaattg aaatgaattg cagattcatt ttttggattt gagaaaatat
     4021 caattctgac aggataaaat tcaaatttag taaatcaatt atttaaaaat atgtaacaaa
     4081 gaataatatt aatatttgat cattttatta ttctgaattt caataataaa tatacccgag
     4141 caataagaca tttttttata ttcatattta atattatttt cttccagaca ttctgagaaa
     4201 ctgtcaaaaa taatatatcc gccttcctct tcttcatccc agtttttatt tttatccatt
     4261 ttcactatta acattttatc aattgaatca aaagctatgt cagcaattat aaagcagcct
     4321 ttattaataa gttctgaata agatttaatt atttcaattt tttctttttg attaaaatga
     4381 tgtaatgtat aagcggataa aatgcagtca aatctttctt cttttaattc atccggtatt
     4441 cctttcgata tatcgtatct gatcaaattt tttgatttaa atttagatcc agctatctcc
     4501 agcattttct ctgaaaaatc tatgcctata catttttgat tttcatcagt taccttgctc
     4561 agcatatatc cagatccaat cccaagatca agtatatttt tcaaattctc ttccgatatt
     4621 tttttcttaa tcacctctat aatatcaaag tatcctgcaa aaggatactg atccttcaaa
     4681 agattaatat cattctcata attttttgcc cactgatcaa atttagtttc tttcattttc
     4741 agttatttaa tataattttt gagatttaga attaaaaaat tatttgttaa aggaattcat
     4801 atattattta ttctgaattt atgaataatg taactcagtt agaattgcta atcaattatc
     4861 aaatattgct taactataga gcaaatgttt ttaatttatc cgaaattacg agtacttttt
     4921 attaattaaa ttaaatgatt tataagattt aaaaaattta aataattaat ctaattataa
     4981 tgattaaaat gaataaaatg attaaaatga tttttacttt tcaatttaca tcctagatct
     5041 tctatttcta gttagaaatt tcaaatttga aattgatctt tgaaaaatgg tcatttctac
     5101 atgctgccca aaatgtaatt cacgcatgtt tttagcggga attcattcga aaactttcca
     5161 ttttatctga atccggatta ttttaaactt aaaagatttc cgggatataa tgtaaacaga
     5221 attctttaga ttgcattatt tcaaaatcct gttaatcttt taatcctgaa aatcctggat
     5281 tagacaattt gatttttctt caaaagaccc aataaagtaa attgaacaag gaatgtaatt
     5341 tgagtatatt tgacgtactt atgctcaaga ctatttcccc gttcatattc ctgcttttta
     5401 taagcattaa tactttcgca tatgactttt ctgaagtaga tcaggtcatc tctgcgggta
     5461 tatataatca ggcctatccg ggagctcagc ttttggtggg aaatgggaat gaaattttat
     5521 attcaaagtc ttatgggaat tttacctatg atgaattttc gcagccggtc actgaaaatt
     5581 caatgttcga tctggcttcg gtcacaaaag taattgccgc tacatctgca attatgcttt
     5641 tatatgaaaa tgaaaagatt gatctcaatg atccggtgag taagtacatt cctgaattta
     5701 caggccgtgg caaagataat attactatag tagatctttt acttcatagc tcaggattaa
     5761 ttgcatggat ccctttttat aaaacctgtt catcaaaaag tgatgtactc aatacaattt
     5821 acgatcagga tctgaattat tctacaaatt cgaaatcagt ttacagcgat ctgaatgcaa
     5881 tacttctcgg cgagatagtg gaaagagttt cgggaatatc tttagataaa tattgcgaag
     5941 cgaatatttt caaaccgctt ggaatgaaaa atactactta tgtacctgct gatgaaattc
     6001 agaaaaacat tctgccgaca gaaaatgaca catactggcg cggcagactg cttcagggtg
     6061 aagtacatga tgaagctgca gcagtgatgg gaggagtctc aggtaatgca ggattattct
     6121 caaatgcagg agatgtatat atttttatga agatgatgct gaacgacggt cagtattata
     6181 atccttattc acggggattg aaagcagaat atatgttcaa tcctgaactt ataaatcttt
     6241 tcacatctaa gtttacagat cttggttatg aaaattcacg tgcattaggg tgggatacaa
     6301 aacctgacgg ctcgaattca aaatacagaa ttccctgcgg tgaacttata tcagaaaact
     6361 gttttgggca tacgggatat accggtacaa gcgtatggtg tgacgtggac agaaagctga
     6421 taataatttt tctgacaaac agagtctatc cttcaagaga taataacggc atcagagata
     6481 taagacctga ggttcataac aaagtgatag aagcagtatc taaataaagt caaaattaaa
     6541 ttaaacttaa ataatattaa atcatgagat tagcaaaatt tcaaaacgaa ccatttacag
     6601 atttttctaa gaagaagaat gcaaaggatt ttgaaaaatc tttattgaaa gtcagagaaa
     6661 gattttcaaa agaatatcct ttagtaatag gcggagaaaa ttttacatcg gaaaaaaaat
     6721 tagtttcata taatccatca aagagagatg agattctcgg aacatttcag aaagcaactg
     6781 ctgagatggc gatcaatgca attgaccttt cgcataatac ttttcaggaa tggaaaaatg
     6841 tacctgtaaa aaagagagtt aaaattttca tcgaagcagc gaagaagatg agaaagagaa
     6901 aacatgattt ctctgcaatg atggtatatg aagtcggtaa gaactgggct gaagctgacg
     6961 gcgatactgc tgaggcaatt gacttcatag aattttatgc aagagaaatg ctcagatacg
     7021 cagaaggtca gccggttgta aaatatccgg gtgagaaaaa cagactcgaa tatattcctc
     7081 ttggagtcgg tgttgtgatt ccgccgtgga atttcccgct tgccatttta gtcggaatga
     7141 caactgcttc gatcatcaca ggaaatactg ttgtattaaa accatcaagc gactcccctg
     7201 ctattgctca gatgtttgtc gagcttatgg aagaatgcgg attgccaaaa ggcgtgttga
     7261 ctttccttac aggtgacggt tctactatcg gtgatttaat tgttactcat ccgatgacaa
     7321 gatatatttc ctttacagga tctatggaag tcggattaag gatcaatgaa cttgcagcta
     7381 aaactcagcc atcacagatc tggataaaaa gagttgttgc agagatgggc ggaaaagatt
     7441 ctatcatagt ggattcagaa gtaccggatt tttatgaagc agtgaaaggt acagtgtcgg
     7501 cagcattcgg atttcaagga cagaaatgtt cggcatgctc gagagtgata gtagatgaaa
     7561 agatctacga taaattctgt gaagcattag tgaccgaaac tgaaaaaata aaagtcggtg
     7621 actcttctct aaatccaaat atgggtcctg tcataaatga aaaatcaaag aacagtattg
     7681 caggatatat taaaaaggct actgatgaag gcggaaaatt aattcacggc ggtgcagaga
     7741 tgagtgttga tgggtattat attcagccga caattattaa agacgttgaa agaaatgcta
     7801 ctattgcaca ggaagaaata ttcggaccgg tactggcagt tatcaagtca aagaattttg
     7861 atgatgcaat ggaaattgca aataatacca tctatggtct gacaggtgcg gtttttacaa
     7921 agaataaaaa gaaacttgaa agagctgaga aagaattttt tgtcggcaat ctttatctga
     7981 acaggaaatg tacaggtgct ttagtaggag ttcatccttt cggaggattt aatatgagcg
     8041 gtacagattc aaaagccggt ggccgggatt atctcggatt atttatgcag gcaaaatcta
     8101 ttgcgcagaa aattaaatga acaattaagt cattttaatc aaaattaaat ttatcataaa
     8161 cttaattatt taaatcaaca tgacaaaaat atcagtgtca ttaatatttc tttttttagc
     8221 ggtgacagtt tacgcctgcg gtatcagaga aaatattgaa aatagagtaa atgaaaaaat
     8281 aaatgaaaca gtagataaga gtcttctgaa gatagactct gcacttgata atactaagac
     8341 catgcttgat acaatgggga ataaaacatt cgaaggaatg gattccgtca agtatcaatt
     8401 agactcagtc agtaaagaaa taaaagaaat gattgagaag caggaaaagt tattaaaaaa
     8461 atagatcata aaaattaaaa gtatttaata aaaaaaatat aaataaatga acttacatga
     8521 gtatcaggct aaatcgattt taaaaaaatt aggaataaac gttcaggaag gtgttgcatt
     8581 aatggatatg cagtactttg acgaagcaat agaatatctt aagtcaaaag acattaagaa
     8641 atatgtcgtc aagtcgcaga tacatgcagg cggcagaggt aaaggtgttg tttacagaaa
     8701 agataaaccg gatgagatag tagtagaagg cggtgtaaag tatatgggta atgatcttca
     8761 aaaagcaaaa gaatatgccg gcaaacttct tgggaatgta ctggtaacac atcagaccgg
     8821 taaagaagga aaacagataa atacaatttt tattgctgaa ggtctcgaca ttaaaaaaga
     8881 attttatatc ggaatacttt tagacaggtc agtttcccgg aatgtcataa tggcttccac
     8941 tgagggtgga gttgaaattg aaactgtagc tgaagagact cctgaaaaaa taattaaagt
     9001 atgggttgat ccttctgtcg gactgctttc atatcaggca cgtgagcttg cattaggact
     9061 aggacttgaa ggtaatgttt ataaagattt tattaaattc attacaaaac tttacaatgc
     9121 atatgtgacg atggatgcct cactgcttga agtaaatcca atggtcatta ccaatgatga
     9181 taaaatcgta gcacttgatg cgaaagttac actagacgga aatgcagatt tcagacataa
     9241 agattacaaa gaactcgaag atacaaatga agaggaacct cttgaaacag aagcaaagaa
     9301 acataatcta aattatatta agctcgacgg aaatgtcgga tgcatggtaa acggagcggg
     9361 acttgctatg ggaactatgg atcttataaa acttgccggc ggatctcctg cgaactttct
     9421 tgatgttggc ggtacagcaa cacctgacac tgtcgaaaac ggattcagaa ttattttgtc
     9481 agatccgaat gtaaaagcaa ttcttataaa tattttcggt ggaattgtaa gatgtgacag
     9541 agttgcaaac ggtgtcatac aagctgctaa gaatattgat ataactgtgc ctgtagtagt
     9601 aagacttcag ggaactaacg ctgtcgaggc aaaagaaata cttgataact caggactcaa
     9661 tcttatctca gctaccactc ttgaagaggc agctaaaaaa gttactgaag cattaaatta
     9721 aatttctgtt acactgaatt taaaattatt tgaattcaat ataataaact tatttgatct
     9781 aaacacagat ggaaaaaatt aatatatcca aaaacggcag caggacttac agcagtaaaa
     9841 tatcagagac atattcattt acgtatgatg atatttcttt actgccggat cagatctcag
     9901 aaattgaaca cagggctgaa tgcgatacga gtgttgattt tcttggaata aatttgtctc
     9961 tgcccataat tggttcgccg atgaatactg tagttggagg cagaatggct aaggctttag
    10021 ctgatctcgg atgtctcggc atagtacaca ggttcaatac tatagtagaa cagataaagg
    10081 aatttacaga taatgatatg gccgactcag atttcaaatg tccggcaata ggaataaata
    10141 aggacactga tctggtcaga ttaaaaagtt ttgctgacat tggagtgaaa gtagtttgta
    10201 ttgatattgc aaacggcggc tcgaagatga tagaagaatt tatttcttcg gcttcgctca
    10261 caatttcaga ttataaaatg aagatcattg caggtaatgt tgcaagttac gaaacgacag
    10321 aatttctcat aaacatcgga gtagatgcaa tcagagtcgg tattggaaat ggtgcaatgt
    10381 gttcaacttc tgtaaaatca ggtatcggta tcggacaggt ggatgcgatc ttaagatcac
    10441 ttgaagcaaa gaacgatctc aaaagtgatg tgaagattat tgcagatgga ggaatttcca
    10501 ctccgggtgc aatgtgtaaa gcaattgcgc ttggctgtga tgctgtcatg tttggaagag
    10561 tattatcagg tacggaagaa tctccgggcg atgttttgaa atacaataat cagcggtgga
    10621 aaaaatattg cggcagtgct tcatttgcag tcaagcagga taaaaaaaat tatatagaag
    10681 gtgaagaatc actcgttcct tacaaaggtc tggtctcaaa gatcataact gaatacaaag
    10741 aaggacttca gtcttcaatg agttatctta attcgagaac tataaaagag tttcaggaaa
    10801 aggctgcttt tgtgaaattg acaaacagtt cattcaacga aagaaagccg cagatctgat
    10861 gcattatatc ttttggttca gaaaagatct gagaactgaa gataataaag gtgtgtctga
    10921 atttacaaag gtagtatcag ccggtcataa gttttcatta atatatataa agaataaaaa
    10981 cagctttaaa tattttggtg aaaagagaat ttcattttta cataattcac ttgctgaact
    11041 ttcagatgta attggaacaa agaaactaaa gcttcagatt ttatatggca acagtacaga
    11101 aatttttaga tttcttacag aaaaatatgg gagtatttct ttatactata atatacaggt
    11161 agagccatac tgcataaaaa gagattcaga tgtttcagaa attatcggga agagcggcgg
    11221 aaatgtaaac gagtttactg attctacaat ttttactccg ggagaaattc aaaatctttc
    11281 aggagatcag tacaaagtct ttactccttt taagaattat gtacttggaa gattaggaga
    11341 tagacatttt gctaagatca aaactgattt cagcagtctg gatatttcaa atgaacttat
    11401 cattgcccaa aattcggtta agaaagttca gtcttttgaa aataaaaagt tacaacaggg
    11461 aattcttaaa ggcggcagaa aggaaggact cgttctttta aaaaagtttt atgagaatgg
    11521 aattaagtat tataagaaaa acagagatta tcctgcggta aaaggtacaa gctatctttc
    11581 agctcatctg cattttggaa ctgtcggaat cagagaagca ctgagaactg ctaaagctgc
    11641 attgaaaaat gctaaggatg aatcttcagc aatcggagtt cagacctgga taaatgaatt
    11701 gctctggaga gaattctatt atcacatgac tttttttaat ccggatctga ctttcagatc
    11761 ttttaaaaag gaatgtgatg atctgaactg gagctatgat gaaaatgatt ttgaaaaatg
    11821 gtgcagcggt cagacaggat atcctatagt agatgcaggg atgagacagt tgaataatga
    11881 aggctggatg cataacagac ttagaatgat tacagctatg tttcttacaa aggatctttt
    11941 tatagactgg agattaggtg aaaaatattt tgcagataat ttgattgata tggattttgc
    12001 aagtaataac ggaggctggc agtggtgtgc ttctacaggt gcagatgcgc agccgtattt
    12061 cagaatattt aatccatatc ttcagagtaa gagatttgat acaaacggag attacataag
    12121 gaagtatgtt cctgaactta taaatgttcc tttaaaaaat attcacgaac cgcatctgat
    12181 gagtcatgat gatcagaaaa aatgtaatac tattatcgga aaagattatc ctttaccaat
    12241 ggttaaacat tcgaaagtaa aaaaaagagt tttaagtgaa tttaagaagc tgaataaaaa
    12301 ataaatttat ccaaattaat tggaatctga aattaataaa tataatagat acgaacctgt
    12361 aatcggacta gagatacatg ctcagatgaa aacgaattct aaagcatttt gcagatgctc
    12421 tacgaatttt cattcatctc caaattcaaa tgtatgtccc gtttgtctcg gatacccggg
    12481 agcattgcct gtactgaatg cagagctagt cgattatatt gtaaggatgg gaattgctac
    12541 caattgttcg ataagagaag tttcaatttt tgcgaggaaa aattattttt atcccgacct
    12601 ttcaaaagga tatcagatca ctcagtttga tacaccgatt tgctatgacg gtcatcttga
    12661 tattacatta aagaataaag aagaaaagaa gatcggcatc acgcggatac acatggaaga
    12721 ggatgcaggt aaatccatac atgacttttc tgataatgat tcattaatag attttaacag
    12781 atgcggagtg ccgcttattg aaattgtaag tgagccggat atcagatcga ctgaggaggc
    12841 ttttttatat ttgtcaaaga ttaaacaaat acttatgtat cttgaaatca atgacggaaa
    12901 tatggaagaa ggttcgctga gatgtgatgc gaatatttct gtgcgattaa atggtgctga
    12961 agaatttggc acgagaactg aaataaaaaa tctgaattct ttcaggaatg tctctgacgc
    13021 aatcgaagct gagatcaaaa gacagatcga tcttttagaa tcaggagaga aagtcattta
    13081 caatacaatg acatttaatg caaaagataa aaccactgct gctacacgat ctaaagagga
    13141 agcgcatgat tacagatatt ttccagagcc tgatctggta aaggtcagga tggatgaaga
    13201 atggatagct aaaataaatt cggcactgcc tgaatttcct gatgccagac gagacaggta
    13261 tataaaagat ttttcactcc cctactcaga tgctgatgag atcacacagg ataaatactt
    13321 tgcagattat tttgaaaatg ctgtagataa tctttccgat aaagaaagtt cttcatataa
    13381 aaatgtaagc aatttagtaa gatcggacgt gagacgaata ttgaatgaag aaaagatcaa
    13441 cattgataaa ttcactgtcc ctgaaaagat cattgcaatg atagcagagt cagtaacttc
    13501 aggtaatgtc agtgcaactg catcaaaaga aatctttaat gcagtactga aaggaaattc
    13561 tgaagatgaa atgttaagtc tttttaaaaa agctgaatcc gatttttcgc aggtctctga
    13621 tagttctgaa ctggaatcaa tcgttaaaaa aatattagag gacaatccga aagaagtgga
    13681 tagatataag agaggtgaaa agaaattagc tggattattt gtagggaaga taatgcagga
    13741 aagtaaaggt aaagcaaatc cgaaaattgt gaatgaattg ttaattaaat ttttagaaat
    13801 cgaaaccgaa taatttttta tgagaagaaa attaattgca ggtaactgga aaatgaataa
    13861 gagctataat gaatctcaaa ttcttgcaga agaaatttca aaaaatattg agccaactat
    13921 tacagaaaat acagatgtgc tgatctgtcc gccttttatt tcacttggtg tgatttcaga
    13981 aattatcaaa gattcaggaa taaaactagg tgcacagaat gtatattcag aaaatgacgg
    14041 agcatataca ggtgagatct cggctgaaat gttaaagtca gttggatgcg aatatgttat
    14101 tatcggacat agtgagagac gaaaattttt ttatgagacc gatgaagatg ttaatgcaaa
    14161 agtaaaaaaa gctttagata atggactgaa agttatagtt tgtgtcggtg agactttgga
    14221 agaaagggaa aagggtgatt ttgaaaaagc cgttactgca caggtcacag gtgctttgga
    14281 agaaatttca aaggaaagta tgcgggatgt tgtgatagct tatgaacctg tatgggcaat
    14341 tggtacagga ctcaatgcaa cacctgagca ggcaagcgaa attcatggaa tgattggaaa
    14401 tacagtttca aaattatatg atagtgacac agcagaaaat ctcaggatat tatatggagg
    14461 aagcgtgaat gcgaagaatg caaaagaaat gctatctgca tgcggagtag atggagcttt
    14521 gattggcggg gcaagtctta agcctgatga ttttattagt attgcaaatg aagcttcaaa
    14581 aataaaataa attttttaat taagtaaatc aaaaataata aatgatagag acagtcatag
    14641 tagcggcagt aaggacgccg gtcggaagtt atatgggaag tttatcttct ctgaaaggtc
    14701 ccgaactcgg ggcaattgca ataaaagaag caatcataaa agctaatata aaacctgaag
    14761 atgtttccga agtaataatg ggatgcgtac ttccggcagg aatgggacaa gcaccggcaa
    14821 gacaggcagc gatatttgcg ggaattccgg attcagtacc ggctctgacc attaacaaag
    14881 tctgcggatc aggattgaaa tctgtgatgc ttgcagatca ggcaataaaa tgcggggatg
    14941 ctgaaatagt aatcgccggc ggatttgaat ctatgagtaa tgtcccatat tatctcgaca
    15001 aagcacgtga aggatatcga atgggtaacg gaactttgat agacggaatg gtgcatgacg
    15061 gactctggga tgtgtataat aattttcaca tgggaagcgc tgctgaaatg tgcgcaggag
    15121 aaatgaatat ttcacgtgag gaacaggatt catttgcaga ggaaagttat aaccgagcga
    15181 tcaaagctac taaagatggt ttgtttaaag atgagatcat tcctgtagag atcaaaggaa
    15241 aaaaaggaag tgtgatagtt tctgaagatg aagatcctac caaagtaaat tttgaaaaag
    15301 gtaaaacttt gagaggagca tttgataaag atggaacagt aactgctttc aattcttcca
    15361 gtctgaatga cggagcatct gctttagttt tgatgtcagc tgataaagca aaagagctgg
    15421 gattaaagcc acttgctaaa attgtagcac aggcatcatt cgcacagaag ccggaatggt
    15481 tcactacttc tcctgcttat gcaataaaga aagttcttga aaaagcaaaa cttgaagtga
    15541 aggatcttga tcttattgaa gctaacgaag cattttcagt acaggcattt gcagtgaaca
    15601 aaatcgcagg tcttgattcg gcaaaagtaa acgtaaacgg cggagctgtt gcacttggac
    15661 atccgatcgg cgcaagcggt gcaagaatac tgactacttt agttcatgct atgaataatc
    15721 gtgatgcaaa aagaggactt gcgacattat gcattggcgg cggtgaggca agtgctttga
    15781 tagtagaaaa agtataatta tttttaaaaa aatttataga attttaaaaa gtataaaatg
    15841 aataatatag ctgtaatagg ttcaggaaca atgggtaacg gtatagctca tacttttgct
    15901 ttgtttggat ataaagtaaa actcattgac ataaaacagg aatttttgga taagtcaatt
    15961 gcaactatca caaaaaattt agacagacag gtgagcaaag gttcgctgac tgaagatcaa
    16021 aaagtatcag cattaaaaaa tattgtcacc actgtaaaca tgtctgatgc atctgacagt
    16081 gatctggtag tggaggcagc tactgaaaat attgaaataa aaaagaagat cttttcagag
    16141 ctggataaat gctgtggtgc tgatgcagta ttagcgagca atacttcatc aatttcgata
    16201 actgagatag gagcatttac aaacagacct gataaagtta ttggaatgca tttcatgaat
    16261 cctgtaccgg taatgaaact tgtcgagatc atcagaggac ttgctacatc tcaggaaaca
    16321 tttgataaaa taaaaactct taccgaaaaa atcgaaaaaa ctcctgtcga agttcaggac
    16381 tatccgggtt ttgtagcgaa cagaatttta ctgcctatgc tgaatgaagc aatgtactgt
    16441 gtaatggaag gcgtcgcaac tcctgaagct attgacaatg taatgaaact cggtatgaat
    16501 catccgatgg gaccgctgac tcttgctgac tttataggac tggatgtttg tctttcgatt
    16561 atgaatgttt tgcatgatgg tcttggagat tcgaaataca gaccatgtcc cctgctaaag
    16621 aaaatggttg ccgcaggtta tcttggcaga aagaacggaa gaggctttta tcattattca
    16681 caaaaataaa tttaaaatta atttaaataa aatataatat gaactttgat cttacagaag
    16741 agcagatctc gattcgggat gctgcaaaag attttgctga aaatgaaata gctccgtctg
    16801 cagttgaaag agatttgaat gctgaatttc ccgctgagat tgtaaagaaa cttggcgagc
    16861 ttggttttat gggaatgatg gcatcacctg aatggggagg agcctgtatg gatactgttt
    16921 cttatgtact cgctatcgag gaaatctcga aagtagatgc atctgtagga gtgataatgt
    16981 ctgtaaataa ttctttagta tgttatggtc ttgaaaagtg gggcagcgat tttcttaaag
    17041 aaaaatatct taagccgctt gcatcaggtc agaaacttgg agcattttgt ctttcagaac
    17101 cggaagcagg aagtgacgct actcaacaaa aaactgaagc tgttcttgaa ggtgatcatt
    17161 atattattaa tggaattaag aactggatca caaacggagt ctattgtgat ttttatttcc
    17221 ttcaggcaat gaccgataag tccaaaggtt ataaaggaat ttcaactttc ataattgata
    17281 aagatacacc cggtcttgag gttggaaaaa aagaagacaa gcttgggatc agaagttctg
    17341 atacgtgtac attaggttta aataatgtaa aagttccaaa ggaaaattta attggagatg
    17401 aaggaattgg atttaaaatc gccatggaaa cattgaacgg cggaaggatc ggagtagctg
    17461 cacaagcatt aggaatagct caggcaagtc ttgaggcatc tgtaaaatat tccaagcaaa
    17521 gaaaagcatt tgattcaact atttctgagc ttcagtcgat tcagtttaag ctttcagaaa
    17581 tggcaacaag tatagaagct gcaagattga tgatttacag cgcagcaata aataaagaca
    17641 gcggtaaaag ttatattaaa gaagcttcaa tggggaaact tcttgccagt caggctgctg
    17701 tgaattgtgc acttgaagca attcagatac acggtggtta tggttacgta agagaatatc
    17761 tggtagaaag atatttaaga gattccaaga tcacacagat ctatgaaggt acaagtgaaa
    17821 ttcagaaact tgtaatagcc agagagctgc ttaaaaactg aatataatat ttgccgaaag
    17881 atttacgctg ttgaagtttg aagatatagt tgttatcaaa ggtttattta catcattatc
    17941 taaaggtaca actctgtaac tcagctcaaa tccatagccg atcttggagt agcttgcaag
    18001 acctattgca aactcatagt taaaattcca aaattcgtct tctgctctag ctgagccgat
    18061 gtaattatag ttaatatcat cagtataaac ctgtgcaata atttcttcag aaaaatacat
    18121 catacctgca cctgcgctta tatatacttt atttaaaaag gaagtataat tttttccctg
    18181 cgggaatact tttactttta gatttaatgg cagtgcaaat aatcttgaat cagtcggtac
    18241 ataaaaccga cttccattta ctgcaccagt ataataaaat ccatcagttg aataagaggt
    18301 cgtaaacata tatgccggtg caaattcagc tgcaattgtt tcactaaata ctgctccaaa
    18361 ttgtccagta atctctttca taaaatctcc gctcgataac tgaaaaaaag gaaaagcatt
    18421 tcgggctgta ttatcagtaa gaaatacaag tttgtatcca ataccagcgt agtaatttat
    18481 attatccgat ctgcttttta tctgagaaaa tgaatttgaa ctgataaaaa gtatcagggc
    18541 tgtcagaatg tattttttca taaaaatttt attaaatgac taaagtatat atctgctgag
    18601 atctctgtct tttacaatca gatctaattt tttacagaca tactcttttg taatttcgat
    18661 tttttttgtt ttaaatttat caggaatttc gaataaaaga tcttcaagta ctgtagtcaa
    18721 aatagtatga agtcttctcg caccaatatt ttctacctgc tcatttacct gcgctgctat
    18781 tcttgcaatt tctttgatag cttcatcctg aaatgttagc tttactcctt cagtatcaaa
    18841 catcgccata tactgtttta tcaaagcatt ttccggctga gtcagtattt tataaaattc
    18901 atcctcaccc agactgttaa gttctacccg gataggaaat cttccctgaa gctcaggtat
    18961 cagatcactg ggcttggaag agtgaaaagc accagaagct ataaataaaa tgtgatccgt
    19021 aactaccggt ccatattttg tgatcacagt cgaaccttct acaatcggaa gcagatctct
    19081 ttgaacccct tctcttgata catccggact ctgtgatttt gcattgctgc ctgcgacttt
    19141 atcgatctca tctataaata ctattcctga attctgaact ttatcagtgg cttcttttat
    19201 cacagcatcc atatcaatca atttaccgga ctcttcctgt gacaaaacct ttcttgcttc
    19261 agcaattgat aattttcttt tcttggattt ctttggcatc atattaccaa acatgtcatt
    19321 gagatttatt cccatttctt ctagaccaat tggtccaaga acctgcagca ttgggaagtt
    19381 atcactcgaa agatccagct ctatcatcct gctgtccagc gcgcctttcc ttaactgttc
    19441 acggagtttt actcttgtcc ttgcattcaa ttcagcagct tcagcttttt catctttact
    19501 ggctgcttca tcatcagttc catttgcaga ttttttcata ggtggaagta gtatatctaa
    19561 aattctttct tcggcatttt ccatagcctt aatttcaact tcctccatct tatgtgtctt
    19621 tacaatattc accgaaagat ctgtcagttc acgtatcatt gattcgacat ctctgccgac
    19681 atatccaact tctgtgaatt ttgaagcttc tacttttata aaaggtgcgt ttgcaagctt
    19741 ggaaattctg cgtgcgatct ctgtcttacc tacaccggtt ggtccgatca tgataatgtt
    19801 atttggcatt atctcatctt tcattttgtc tcccacctgc tgtcgtctcc atctgtttct
    19861 caatgcaatt gctacagatt tttttgcatt ctgctgaccg attatatatt tgtccagttc
    19921 ttccactatc tctgaaggag taagctgtct gatctcctgt ttcactttta cttctttttt
    19981 tgctgtagct ttttttggtt tatcttttgt gatcactttt tcttttatca atttaatttt
    20041 ttcgttttaa tataattata attcttcaat agatatatta ctatttgtat atatacagat
    20101 atctgctgcc tgtttcaatg actcttctac tatttctttt gcggatagtt tggaatattt
    20161 caaaagcatt ctcgctgata caagagcaaa ctgaccaccc gaaccaattg caacaatgcc
    20221 gtcatccggt tcaattatat caccggtacc tgaaattata agagcttttt cactgtcaat
    20281 gatcgcaagt aatgcttcaa gtcttctaag atatttatcc gatctccatt ccttagccag
    20341 ctccactgcg gctcttgaca gatttccctt atattgatca agcttgcttt caaatttttc
    20401 aaataacgta aatgcatctg aagtagatcc ggcaaatcct gcaagtactt ttccattata
    20461 aagttttctg accttcttag tcttatgctt cataactgta ttccccattg tcacctgacc
    20521 atcactgcct atcacagctt tgccgtcttt tataagtcct atgactgtag tcgatctgat
    20581 tctatttttg tttttaattt tcaattttta tatttaatta attatttcgc gtctttgttt
    20641 tgcgatcggc agatctacag actctctgta ttttgccact gtacgtcttg caattttcaa
    20701 tccggacttg gttaatattt ctgatatctc ctgatctgta agaggatttg atttatcctc
    20761 tttttcaatg atctctttta ttttatttct cacttcaata gttgagatat cactgccatc
    20821 tttattagga attgaatttg aaaaaaacga tctcatatta tatattccaa agtctgtctg
    20881 aacatactta cctttgactg ccctactgat tgtggaatta tccattctaa tatcttcggc
    20941 aatatctttt tcaaatacag gatttaaatt ttctccgtta ttttcaaaaa attccttctg
    21001 cctgtttata atagcttcca taattttgat catcgtttca cgtctagaat taattgcgtc
    21061 aataaaccac ttcgctttat taaaattgtt cagcagatat tctttggtat tactgtcaag
    21121 gttcttttta tccttggtgt acatatcgct gtatgtccgg ctgatcctta atgaaggagt
    21181 aaacttttca ttcagataaa cttcatattt atcttctgtt ttttttacaa taagatccgg
    21241 aacaatgtaa cccacattcg catcagtatc ctgtgaatag ggttttggat taagggtatg
    21301 tacaaattca aatatctcct ttacctcttc catatttata tccagctcct ttgaaagttt
    21361 ttcaaaccgc ttatgtttca tatcatcaaa atattctgta aatattttaa tcgtattctc
    21421 ttttattcta tcatttatgt tcgatctttt tacctgaata atcacgcact ctttcagatc
    21481 acgtgcagca attccgggtg gatcaatatt aacctgaata tattcaagca tattcaaaat
    21541 atcctcttct gaaaaattgt cattttcaaa ttcagtacct ctttttttta catctgtatc
    21601 ttcgagtaca gcttctaaag attccttaaa atatccgtca ttgtcaagag accatattat
    21661 ttcttcacca atgaaaattt ccctttttga aagatcaaac aaatgaagct gaacaattag
    21721 gttgtcatac attgattgtt cttcttcacg aatattattt ctgtcaaatt cattttcact
    21781 accggcaccg cctgaagtat aataaggttc attatctgat tcattttcaa aatattcatc
    21841 ccagttatat tcatcatcct tatcataatt ttcttcagta gtttcctctc ctttaatttc
    21901 agtagtttcg gaattttgtt cagcagactt ttcaatatcc tgatctaatt ccggtccttc
    21961 ttcaagcatt ggattgagtt caagctctcg tttgattatt gtatctagag caagtgtagg
    22021 tatggcaagt atattctgat ttaatacaat ttttggaagt aacttttgcg ataatttttg
    22081 tgatagattt tgattcagca ttatttatat ccgaactttt cttttaaatt tttataaaat
    22141 tcttttccgt attctcgggt cagagattct tttagaaaat cgatcatttt gatattcttt
    22201 tcttttcctt catcaagggc atcacggcat tcatagatct cttcgtatct caatgtattt
    22261 cttttctttc ctgaaattct tatcggaaat aactgacagg atattggttt tcggaattta
    22321 atttcatcat taaaataagc tctctcaaaa gcgcatttag caatcccctc ttcacgaaat
    22381 acaaacacac aatcttcatc atccatactt ttcagttcaa gatcatctcc tgtaccattt
    22441 gtaaatccat cctgatcaat gatcctattt ttttcttcac agagatattt cttgacagat
    22501 ttcaaagatg atttgatctc attgatctcc ttttctaaaa gcggagcccc gcccgcacct
    22561 tttaatgtac agcaggcacc tttgcatttt gaaatattac aggcaaaatc agtagtaaat
    22621 attctgtcgt ctattttaaa tttttccaag tcaaattctt tataatctca taaaattctt
    22681 tcccggtaat tgtcttatca gtcctttgaa ttctaatgta agaagactta caagacaatc
    22741 tgacaccgga catccgcttt cttcattcag cctatcaata ttcttcattt caaatccaat
    22801 cagatcatat aatttttttt cagtactgct aagaaccatt tcttttttat tatctgaaat
    22861 ttctttttcc ttatttaaaa atcttttgag cttaaattcc agttctatta acacatcatc
    22921 tatacatgtc actaactttg cctgaccttt tttgataagt tcgttcgttc cgtcagactg
    22981 tctggaatta atataccctg ggacagcaaa tacttctctg ttctgatcat ttgcaaaatc
    23041 tgcagtcaaa agtgaccctc cttttatacc gctttcgata attatacttc ctatactgat
    23101 gccactgatg atcctgttcc ttttgggaaa attcatttta tccggtattg atcccggcga
    23161 aaattcagaa ataaaaactc cgttattaat aatttcatca tacaatttcc tgttttcagg
    23221 cggataaata acgtcaattc cgcatcctaa aactgcataa gtaagattac cttgtttaat
    23281 acattctctg tgagcgatcg aatctattcc ccttgcaaac ccgctgataa ctggaatgcc
    23341 aaattcagaa agctgctgag caaatttttc acaggtatat tttccgtatt ctgttggatt
    23401 tcttgtgccg acaatgctga gtgaatatct gtcagacttt tcagtcttac ctttaatgta
    23461 gagtaaggca ggagcatcat agatcatttt aagattttcc ggataatcat tatcaagaat
    23521 ggaaacaatt cgaatacctt ttttaatgga tctgtcaaat tccttttcaa attcatcatc
    23581 ataaaaagat cttttttcgg tttccctgat cacttctgca gagactgaag aatcaattcc
    23641 atctattaat ttcagatcat ttctgctgca attaaaaaaa tcatcttcgt caggaactgc
    23701 cttcagaatt ttctttatcc tgttatttcc aagctttttt acctttgtaa gaaaatatat
    23761 gtatttaagt gaaatcaatt gtctcccgtt taatctgtat taatctactc aaatttatgt
    23821 attaaaaata tacaaaatat ttatacttgc aaaaaccgtc gggaaatcat tttaaagatt
    23881 caataaattt attcaaaaaa atttaattca tctcttcaaa gattaaagtt ggagattaaa
    23941 aaatattaca aaaaaaaagc gagcccaaag acccgccttt ttttaactaa ataaatttac
    24001 taactattaa ctactaacta ttaactacta actattaact actaactatt aactgctaac
    24061 tattaactac taactattaa ttacggaata gactttgtca caaagttatt agtattatta
    24121 tctgctagtg tagcatcagc cagatccacc aatccatcgc catctgcatc agtgacaaca
    24181 tagcctgtaa taaaactgaa tgcgtcatta tcaatcattc ctccatcagc aagatcgaca
    24241 attccatcct gatttgcatc tgcattataa gctgcatact ttacaggtga attatctgcg
    24301 atgatctgat tacttccgta tgcctgagcc gaagctgttg taaaatcata attcagtact
    24361 gaagctgtaa aactctgacc cggagattta ctccatgtct cgatcagatt tctctgcttt
    24421 agctgcagat aataattcac accgttcact gcattattaa atacataaac acctgttcct
    24481 gtatttgaaa gataaacttt tgcctgatct actacagcat atggagcagt attatttctt
    24541 aaaaaaactg tcatagtatc tcttaccata gtattagaac ccgcattata aaatccctga
    24601 atgtaagctg ttagatttaa tgtagccggt gcagttacat tgataagatt attacctgtt
    24661 ctattctgtc cgtcaggatt tgtaacggta atgttcttca atcctgtcgg tgaaccaacg
    24721 gtcgaaatat ttaaagtaac ctgtgtaggg gaattaaatg taatgctgtt tactgtaaca
    24781 gttcctgaaa ttgatgcagc aattctgttt gcataacccg gtccgcctgc atcattaccc
    24841 ggatcgaaaa attctgaatt agaaattgaa tttccagtga ttacaatatt cactgaagga
    24901 agaccctgca taatgtctgt aacatttgaa gcagaaggag tcgccggtgg cggtgctata
    24961 agttgtattg ctcttacact ccatgaattc gtagcatcac aatattcctg aaaagtccac
    25021 attgtcatgt catcatttgg atcgactaca gtctgtgaat aatctcccca cctctgagta
    25081 gtaccacctt gaactccata aactgaagta ctgctttgag ctaaaattgg tgactgaatt
    25141 gtacctgctg catcagttct gtatctgcct gcaacctgga cttcaggacg gtttgctaat
    25201 cctgcactgc tgcatccgag agccatatgt ccctgtcctg acattgccac acttgggata
    25261 atataatatc gcggatttga ccctgccgga tcaaacaaag ttcctgactg aagtaatgca
    25321 ggagtcgaag tcatatttgt aagttcatac catctgcatc cgtctctgct gccggtggaa
    25381 gatgcaacac cacttgaatt aacttcaata ttatgtgctg tccataatgt ctgagaacct
    25441 gttatcttat ttttatgaag ctcagcagca aaaagtctgt cgtcaagagc atcaagtctc
    25501 ttagttgagc ctgtttgagg agcagtttca ggtgcacctg ttgaaggaac tgtaattgcg
    25561 taatttcctg aaacagaagg agtagcaccc ggattgatca cccttttgac tgaaagtaca
    25621 ctgtatacat atggatctac tccaacaaaa tatccttcag tcgaattcgg atcatcattc
    25681 tgaactccct gcggagtctg tggaccggaa gctccaccgt tagaaaataa tgtaaaagct
    25741 gttacagtaa gtgaacctgc attgagattt gatttattga ccacataacc atttgttcca
    25801 agatatgcag taacattatt attaaacata ttcataccga tatacagagc attattatca
    25861 acacctaatg tcggataatc tgcaaaacct gaaagtgatc cgggtagatt tgctgtgaac
    25921 tggaaaaaag taaaacttgc taagtttgta attgtaggac ctgaacttct ggcaataact
    25981 actctgttag gtcctgcctg agtattaatg gcaaccacat accatctctg tgtaagtctg
    26041 tcatatctga catgcggatc tgaaattccg gctccatttc ttacagaatt aaaaaacaca
    26101 tcaagatcag aattcagtcc gccaacgaca cctgctttat tatacacttt tattctgccg
    26161 ttagctgcga tcaacacctg tgtaggtcct acatcacctt gtgtatcagg cggtaaatat
    26221 cctgattcag ataattttgg cccctgaaaa ttagtcccga tagtctgagg attatctgtt
    26281 tgctgaattt cttttttaac ttcatcttca gacagaaccg gccattgaga aacatcaggt
    26341 gcatttggat tgacctgctt ttccggcatt tcaatttcaa attccggatt ggtctttggt
    26401 tttggtttta gataaatatc tgcaggaagc attgcttccc tgtccatgat ttcctggact
    26461 gttacagtaa ttcctggttc accttgtact gggataccca gagtataatc atcctgaagt
    26521 gaaacagtat ggaaagcatt ccactcagga ctcatgttgt tactgcttat tactgcatct
    26581 tcagcagaat taaaagcaaa gaaaattact gtacaaatta ccagcagact aaaatgtaaa
    26641 aaatatctgc ctttcttaag atctgtaatt acttttttca ttgtaaaatt aatttattta
    26701 aaaaaatata aatgttaaat atttgtatgg ggattaattg aggttgttca atctaattaa
    26761 attaatttta aaaaaaaatt gtaaagtata ttgctcaaat aattaaagtt tagatcaact
    26821 tttctcaaaa aaaaaagcga gcccaaagac ccgccttttt ccaactaact aactaactaa
    26881 ctaactattt attactaact aaactaacta actatattta ctaaatatta attattatct
    26941 attaactatt aactattaac taactcttaa cttttaagga gttattttct gtacaaaatt
    27001 attcgcatta ttatcaccta tactaatatc actgagatca actgcgtcat caccgttcaa
    27061 gtcattggtt acataacctg tcacaaaatt agctgcatca ttatctattg atccaagatc
    27121 tcccagatca actgttccat cattatttat gtcaccactg aaattacaat atttagatcc
    27181 aattaagatc tgattacttc caaatgcctg tgctgcagct gttgtaaagt tataagtatt
    27241 gatagttcct ttatccaaaa ttaattcacc acccgcctta ctccatgtct caatagtatt
    27301 tctgtattta gcaactatat aatactgacc tgactgagca ttagggaatg taaaagatcc
    27361 tgtgaatgaa agtgaatcaa taactgctgt cgctgaatct actattgcat atggggctgt
    27421 ggaatttctg agatatactt tcacatcatc agataatgaa agatcatttg tgccgggaga
    27481 atacatacct tccatcagca cagtaagatt cagtttaagc tgagaattat ttcctgtata
    27541 tctcatgacc gcagcattac caccgagtac ccaagcagta tcaccttgag taacaaaaac
    27601 ttttccttgt gtgatagtgt gtgtattttg gaaagtccag tttactcctc cgtttgtagt
    27661 tctgcaaact actgttgtac tgtttccgca ggcataacct gtattagcat ctttgaaatc
    27721 aatgctcgta aaatctcctg ttgcaggcat tgatattaaa gtccatgttg ctcctgaatt
    27781 tgttgacttt gcaactctgt tggtaccact tgtctgacag atataaaccg ttgtatcatc
    27841 cggacatgtt acacttctga tacttccggt taatcctgta gtcagtggtg accagtttac
    27901 acctccattt gtagttttat aaacaggtct gtttgactga ttcattgtag tataacctgt
    27961 attggcgttt agcatttctg ctgaataaat attctcactg cttgcaccgg ttgtatattg
    28021 agtccatgta tttgcaccgt ctgtagtttt aaatacatta ccgccagata ctgtagcaaa
    28081 atttacaaag acccagcctg tattttcatc tataaagtcg caattatata atgtataatt
    28141 agttggatgc ggtttagttt tcattaccca ggaaactcca ccgtctgttg ttttataaac
    28201 ctgactgttc tgccctgtaa cgtatcctgt attttcatta atcattgata tatcattgaa
    28261 atcacctgta actcctatac cgtatgtagt ccagttcgta ccgctgtttg aagaatacat
    28321 tatctgaccg gtagtcgctg tatgaggtct tccaattatc cagtatttac tcgttccggg
    28381 tatagttgta atattaaaat ttaaagctgt ctttagtaaa tcagattccg aattccaggt
    28441 tgcacatccg tcagttgatt ttgcaactac gccgtaatca ccggacaata cataagttga
    28501 acctacatga tccatagaat accagataaa agtagttgca ggtccggata cacttaaaga
    28561 aagcgttgtc cacgttgctc cctgatctga tgatctgtaa agaaaacttg gatcacccac
    28621 taaatatatt tcagtttccg aaactatttt aatctgaaaa taagcccagt caatctgagg
    28681 agtagctatc gactgccagt tagctccgcc gtttgttgtc ctgctgaatt gtccacctgt
    28741 gccgcatgca aatcctgtat ttgaatttaa gaattcaata ttataaggaa tttgaaaaag
    28801 aactacccct gctgattcag tccaggtagt tccgccgttt gctgtagtga ttgtaactcc
    28861 gtttgaatat gaagcagtta cattgttagc atctgatccg cctatagaaa ctgcatagtt
    28921 tgtgttagaa gaaaatacta ttgcccatgt taatcctgca ttagtcgttt ttaaaagctt
    28981 tgttgtaaat gcgttttgac tacctgaagc aatgtaacct gtatttgcat ctgcaaacca
    29041 gactgcccag tttctcttat ttgtttcaat aagacctgca cttactgtat caaaacttac
    29101 accgccgttt acagtcctac ctatatatcc ctgatctcct actactattc cggtattttg
    29161 attaaaaaac cacatgtcat aattctgtgt ggttctcaaa tttgcatctg cttctcctgc
    29221 aaagtgatgg aaataccagt tgttgccgct gttggtagtc tgcatgtaag ttccgtttgc
    29281 acctgctgta acccatgtat tttcatcgac catcttgatc ttcctaagta aattagactg
    29341 tgtagatgga tgcatccatt tccagtcctg tttgtcctgc gaaaataatg ttcctgaaac
    29401 catcaggatc attacaaaag taaaagtaat tttgcgaatc atttgtttaa ttaaattaga
    29461 tttttaaaaa atttaataaa attaataatt tgctggtttt gtttcaaaga cagtttgtta
    29521 aaagaatacc cagtttaatt tttgtaaaat tgcaattatc taataattta tgaatatcca
    29581 atactgagaa atacccattg aaaatctata tatatttaaa tttttttctg cagataatat
    29641 tagaaattaa tgtgccgggt gagtttgaaa attaaggttt gaaaattaag gtttgaaagt
    29701 taaggtttga aagttaagat ttgaaaataa agatttaaaa tttgagatta gaaaataaag
    29761 atttgagatt tgagatttga gatttgagat ttgagatttg agatttgaga tttgagattt
    29821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggaaaataaa taagtaatgg
    29941 ataaataaaa aaattcagaa attaactatt aactactaac ttttaactat taagaacatc
    30001 ctctttaatt ttcaataaat attctttcgc tgcatcataa ttattcggta tctccccttc
    30061 taaaatagct tcttcaattt tgtgtttgaa tactccaatt atcctgcccg gctcaagtcc
    30121 gaacagcttc ataatttcat cacctcttac aggagactga aaatttctta gttcatcttt
    30181 ttcctgaact tcgacaactc tcttttcaac ttttgtataa ttatctaaaa acttactaac
    30241 cttatcagga ttttttgaag taatatcagc tctgcataaa gtcaacaagt caacaagttc
    30301 ttcacccgca tctgctgcaa gtcttctgat cgctgaatct gtgacatcat cttttgcaag
    30361 cgggatcggt ctgagatgca tccgtaccag tttttctaca taatcaagtt tcgtcatcgg
    30421 cagttttaat cttacaaaca acttcttcat catccttgca ccgacttcct catgtgaatg
    30481 aaatgtccat ccggtccctt ctacaaattt ctttgtcctc ggcttagcaa tatcatgcac
    30541 tagcgcagca aatctcagcc acacattatt tgtatttggt acaatattgt caatcacctt
    30601 taatgtatga taaaatacat tcttatgatg aaagtctttt cgctgatcta caccttcaag
    30661 tgcagctatc tctgggaaaa ttatttccat cacaccagtt ctgtataaaa gatccagacc
    30721 gatcgacggc ttatctgtca tcagtatcag cagaaattca tgtgtgatcc tctcctgtga
    30781 aactactcct tcatctgata atctatgccg catttctctg atcgcattaa atgtattttc
    30841 ctcaatctta aaatcaaatc tcgacgcaaa tcttaccgca cgcattatcc tgagaggatc
    30901 atcatcaaat gttttataag gatctaatgg agttctgatt attttatttt caatatcttt
    30961 caatccgtga aaattatcaa tcacttctcc gaagtttttt tcgtttattc ctgcagcaag
    31021 tgcattgatc gtgaagtctc tcctcgaaat gtcttcctct aatgaagccg tctctactaa
    31081 cggtttacgt gaccctctgt cataactttc ctttcttgct gagacaaatt cgattttata
    31141 ttcatcaaca ttcagcaatg ctgtctcaaa atttttataa actgaattta attccctgcc
    31201 aaattctttt gccactgtcc ttgcaaatga aatcccgtca ccggttacca ttatgtcaat
    31261 atcattaccg tcaatttcca ttatcagatc acgaacatat ccgccgacaa gatatgctgt
    31321 tactttattc gtgtcagcaa tacttcctat ctttccaata agttgatttt caattgaaat
    31381 cctcatcagg gaatgatcac cttttctatt ttaatttctc tctcttcatc tttttttccg
    31441 ggaatttcta ttatgtgata acttcgtact ctgtcatctg ttagtggtat agctgatgct
    31501 gagctgttta taattactat gtttccttta gtatagattt ctgaaatatg tgtatgtccg
    31561 tgaaggatca tttttacttt atatttttct aataatttca gaagcttctt ctttccatat
    31621 aatttcattt ttacatcaat accggccagc caaacagggt gagcaggatt ttcttcggtt
    31681 gattcaggca atccgaaatg atgatgaagt actacaatct ttatcatctt tttgattttc
    31741 tttatactca gcagtttttc aagattttca aattccattt tatcaattct gccgttcgac
    31801 ccttcaggat tcttttttat gtcccatctg tcaatactgt tcaatgcaat gatagcaaca
    31861 ttatcaaaaa ttttcagata cggaaattca attgtctctg gaaatgattc attaaaatgc
    31921 tcagcaaata tttttacatt aagatcatag tctgcattgc tgcatttaaa tataaatttt
    31981 aatccttcaa ttcccatttc agctccgcca aaaatatcat gatttcccgg tactaatgta
    32041 aatttatcac ctcttactaa atcataattt ttaagtaatt cttttgcaaa tctgaagtcc
    32101 ttctcattca cattatccac cagatcacct gacaatatta catgatcaca atttttcttt
    32161 ttgatatctt ccattaattc tacaaaagct ttaccgcctc cgttaatgtc atctaatctc
    32221 agatgagtat cagatatgtg tgcaattttg aacataataa tttaatctta atttattaaa
    32281 taatattatt tttttatctg tgcaatttct atttcttcta ttattttatt tgcaacttca
    32341 agtgcctgta aaccatctgt caaagtgacc agaggttctt catctttaat tatcgaatca
    32401 aaaaatctgt tcagctcata cttcagtgag tttacttcag gtatctcagg cttttcataa
    32461 aatatcttca gcttcttatc accttgttcc acctccgtca cagcaaaccc tgtatcgtct
    32521 ttattgtcag acagtctgaa tacttctgaa gtgttttgta aaaagtctat cgataaatat
    32581 gcattcttct ggaacagtct catcttcctc attttattca gagaaattct ggatgcagta
    32641 atatttgcca cgcagccatt cttaaactgt aatcttgcat tagctatgtc aatcttttca
    32701 ctcagtatcg aagctccatt tgcatgaatt gactcaacag gtgatttgac aagattcaga
    32761 ataatatcaa tgtcatgaat cattagatcc tgtaccacag atacatcagt tcctctcgga
    32821 ttgaactgtg acattctgtg cgtctctata aataatggtg aaatattata tgaatcaagt
    32881 gaaagtattg ccggattaaa tctctcaata tgtccgatct gaattttaat atctttattg
    32941 tcggcaagct ttaataattc aactgcctgc ttggtagtct ctgtcacagg cttttcaatg
    33001 aatgtattaa tattctgttc gatacatttt gatgcaagtt caaaatgcgt agaggtcggc
    33061 gtcactatca cagcagtatt gatctttttt aaaagttctt cggcagaact gtttacatcc
    33121 aaattatttt catcggaaag cattttattt ttctctgtat cggtatcata aatccctgca
    33181 agttcaacat cttcgcgctc attgcaaatc tctttgataa gttttaaatg agtctttccc
    33241 agatgaccaa gtccaaacag ccctgctttt attttatgca ttatcttttt cttagtaaaa
    33301 atttattatt taaaatttaa tatttattat ttaaaattat ctgaactagt ctctgacggt
    33361 tctgaatgaa tgatcacgtt agacagtttc ggataatttt ctttcaggct gagatatatt
    33421 ttactttcaa gctgagttac aaaatcatgc acttcgtcaa aagaatttat ataatcaaat
    33481 acacagttaa gagagaccct gatcttgccg tttgtactga ccacctgaat gctgttactg
    33541 gaaagtacat tttcattttc atcaattatt ctgtttacct cccggatcaa ttccgttgaa
    33601 ttatctgtaa tgtcagttgt atcaaaaact atttcacttg gctcgtctat gtggatcttc
    33661 agctgcgaga tcacatctat cttttcaagt atttcttttt ctacatcagt aattatactg
    33721 tgcgcttttg tcagatcatt tgtattgtcc acttctatat gcagctcaga aaaaatttca
    33781 ttattaattc tatgagaata tatgtcatga cattttaatc caaaatcatt tactatgatt
    33841 ctgatcttat cattgatcgt ttcatctttt gtttcaacag gttcagaatg aattacaata
    33901 tcggtatttt cagaaatttc tttaatcctt ttttcgacat tatccattat atcatgtgct
    33961 ttggaaaaag gtactaacct gctgatctca attgtcatat caatatatgt gatggatcct
    34021 gaagtacgga tcctgcagct gtttattcct tctactcctt ttactagtaa cgtctcgtat
    34081 tttatttttt catataatcc ttccggtact ttatccatta ataaatttat ggattttttc
    34141 aacagtttat acccaagatg aattatgaaa atagttacta ctaaagcagc tatcgtatca
    34201 gcgatcctgc tgatattcag ataggtcaga agtaatccga taaggactac tgatgaactc
    34261 cagatatcac tcgagaaatg taatgcatcc gcttccagtg cactcgagtt tgtttccttt
    34321 gcaattgctt taagtttctt tgctctgtta atgtcaatta aaattgtaaa tatgatcgaa
    34381 ccaattatcc agatattaat atttagttca acttcttcct ttataaaaag tctgttaata
    34441 gcttcatata ttataaccga tgaagtaata aaaagaatga aaacctgaaa cagtgctgaa
    34501 aaattttcgg ctttttcatg tccgtaatta tgatctttat caggaggtct ggaagaatat
    34561 tttattgata aaattgtaac gatacataca aaaatatcaa ttcctgaatt aaatacttca
    34621 gtaagaattc caagactacc gctgaaaaaa gcagcaacga tctttatcaa agtaataaac
    34681 actgcagtat aaagagactg cactgctact tttatttttt tatccgaaac tgttttactg
    34741 ctttctttca tttatatacg gtctttcaag attcttaatt aaattttcgt ctttcagtct
    34801 ttgagttaat acaactcctg tcatagttat tattgcacct gcaacaaaaa aataagataa
    34861 tttttcattt aataataacc aagaaaacag aatagtgata acaggagctg tatttgtcat
    34921 ggttgtcaaa gtacttgtct ttattaactt cgtagaatag ctgtaaagaa aataacttcc
    34981 aaatgctact aaaaaagtaa gatgtatata tcctaaaatg acatatatat ttacattctc
    35041 aaatgtcaga ttatttacat cataaagaaa aacaggtatg tacattacaa taccgatcac
    35101 aaatgaaatt gtcatagttt ttaaagctcc gtattttttt atcatcggct gactgaatgc
    35161 aagatacatc gaccatgatg cgacagcaag gaatagcagc gaatctccga gcatataatt
    35221 tacgcccgga atttttggtg taattaaatt ttcataaaag attacaagta ttccgactat
    35281 tgtaagagaa attgttatta actttttcat ttcaaacttt tcatctttct gctttattga
    35341 gatcagatat acaatcaatg gagtgcaggc atataatatt ccagagtgcg atgcttccga
    35401 aagatttatt ccaataagaa aacaaaattg atttattgga attactaaca ttcccaaaat
    35461 aaaaaatcta agccagtctt ttttatcaat tttgaatttt ccttttttgt aatagaatac
    35521 tattaacagg agtaaggtcg ccattccgaa tcgaaggaat gcaagtgaga gtggtgagat
    35581 tgcaccgact acatactttg cagcaatcgg tgtaaaaccc gctgtgaatg ttaaaaaaaa
    35641 tactaagata aattttttca tttaattcac agctttagtg aatttaaatt gtttagaaat
    35701 ataaaatgaa aaagcaaatt aattatttat tccttcatac aaattcattc ccgcaaatgc
    35761 gggaatctgc atgaattcta attttattta ctctttgttc ccacagagtc aagtgctctt
    35821 cggactctgc ggagactgaa taaattaatt actgcttctt attagcaaga gaagctttga
    35881 tcttagcttt tacttttttt gcattctttc tgtgtttcgc gattgctttc ttttttggtt
    35941 tattcatttt ttattttatt aagttaaaat attttttctt atgcaatagt aatgctgtcg
    36001 tcggaataga catcctgaat tgtattcaga agttcaatac cgtctttcat tggtttttgg
    36061 aatgcctttc tgcctgagat aagtcccata cctccggcgc gtttgtttat tacagcggtt
    36121 ttaaccgctt ctttcagatc ggagtcgcct ttgcctgtgg atgctccacc cgaatttata
    36181 agtccgcatc ttcccatgta acagttcgca atctgatacc tagtgagatc aattggattg
    36241 tcagaagata atttatcata gacgaatttg tgcgtcttac caaaattcaa agcgttatag
    36301 ccgccgttat tttcgggtaa cttttgcttg ataatatcag cctcaattgt cacgccaaga
    36361 tgatttgcct gacctgtcag atccgctgat aaatgataat ctttgtctcc ttttacatta
    36421 aaagcagaat ttctcagata gcaccaaagt atcgttacaa gtcccagttc atgcgcatat
    36481 gaaaatgcct gtgctacttc ctgaagctgt gtcgctgatt catctgatcc aaaataaatt
    36541 gtcgcaccta ctgcaactgc tcccatgtca aatgcctgat ctatatctgc aaacattatc
    36601 tgatcatatt tattcggata agataaaaac tcattgtgat ttatctttaa tataaaagga
    36661 atcttgtgag caaactttcg tgatacagat cccagtactc ctagtgtcga agcaactgca
    36721 ttacagccgc cttctattgc cagctttact atattttcag gatcgaaata tatcggattc
    36781 ggagcaaatg acgctccggc tgaatgttcg attccctgat ccactggtaa tatcgataca
    36841 tatcccgttt ttgccagtct gcctgaatta tatattgact gcatgtttct gagcacattg
    36901 ggacttctgt cagaatcttt ccaaaccctg tctataaaat cacttccagg taggtgcagc
    36961 atatctttac tgatggtttt acatttatga ctaagcagat attcattttc tttgccgaga
    37021 atttttaatg tttttttatt catgttaagt tatatattta ttaaattttt tattgtgcta
    37081 atttgcaaat acagattgct tcctttgttc aggtagagtc acgtactctt cgttctctct
    37141 gaatgctgaa tcaaagttgt atcatctcat tacataaagc ttttcgtttc cgattcaatt
    37201 tcctttttca gaatattgat ctcgttttta tttccatccc ttagaacata gaatttattt
    37261 tcttcaacaa gttctgtcct tcttaaaaat gccagtccat aatatttatc atcattttta
    37321 attacacttg aaataaaacc ggtttccctg tcactattag aattaataac tttatcattt
    37381 ctaatgattt ctttatctga aactatactg accatgctct taggaatttt tccctgtgaa
    37441 tcaattctcg caataacttc ctgccctata taacatcctt ttttaaatga aatatatttt
    37501 tccaaaccgc attccattgg attgatctga tcgttaaatt cattttcacc ctcaggaata
    37561 agattgctga ttcttttgaa ttcatattca tcaaaagtaa gtgcagttaa atctttcatc
    37621 agatttctat atacgctcac atccttttcc tcgcaaataa tattaagtgt ctttattttt
    37681 ccatcactta aaaaaaggat atcactttta tttattctgt aagctttatc tttttcacat
    37741 tttatattaa acagatctga ggaaagtgat tcatattcat ctgtaaaaat tgttattctt
    37801 gaataatcac cccgggctat ttctaacttt acatcatcca taattatata tttatcaaga
    37861 tgtgcaatta ttttttcttc aaatccggat gtacaaatga gatatttttc ttccccgaaa
    37921 ttaacaaaat ttacaagatc tattattctt cctttatcac tggtaagcac agtatatctg
    37981 cattctcctt cgggaaaact cctgaagtca tttgtagaca ttctgtttat aaaatccgta
    38041 aggtctgttc cggatgcttt tattaaacct gatttattaa tgaaataact gacgggcaat
    38101 gaaatgataa ttattttttt cttaagtgaa ttttcttatt actataaaaa ttgataaagc
    38161 tatattaaaa attgtaaaac ctgaaaatat caaaattatt aaaatttgaa aattagaaac
    38221 cttttgtata tttgaaatct tctgaattga tccattttga atatttgttg actttggatc
    38281 ttcaggaaaa tgattttcag aaacttcagt gttgtttcct tccatgactt ttttacaaat
    38341 ataaatattt caggagtgag attaaatgaa agaatcttaa ttttaaatta attattttta
    38401 caaacgtcta aatcgttttg agttatttat cagaatattt taataaataa gttatattta
    38461 gcaaaattta ttctaataaa taaatttttt ggacttcact gaattgttta attcttttaa
    38521 aattaaactg aatttatttt taatttttaa tacttattat ttcaacaact cctgaaaata
    38581 atccgaaacc tcagaagata gaatctgctt ccaagaagct cagctccttc agatcgattg
    38641 cactggatat gataattatt gcagtgatta tcgtgatctg tttatttgtt tataaagagt
    38701 tcaataaaga taaagtagtg atcgaagtct tccaggttcc aatggatctc gaaaaacaaa
    38761 atatcaccgg acaggctgtt gtcaataaat tactcgataa cattataaag atcgaaagtc
    38821 aggctgatac ttcttttcca cttcttgact tcaatcctgt aatgtatgat gcccaaatgg
    38881 aaattgtaat accgggctca ggtatatcca taaactctct gatcagacat gtcaaaaatt
    38941 ttcttggaaa aaaacagata aagattaccg gcgaaattgt ggtatcggat aaaaaattat
    39001 tgatgactac ccgcatagtc ggtgaaccgg caatgaccta caccggagat ctgaatagta
    39061 tagattctct tatttatgaa tctgcaaaac atataattct ctatactcag ccttatatgc
    39121 ttgcatatta ttattactat tcttacggtt cggatcactt aaataaagag cttgcaatgg
    39181 aaatgatcaa ctattccatt actcagcctc ctgttactga tgacgttatg gcatacactt
    39241 tgtatggata tattctttct gaccagggga aatatgagga gtcaattaat atgtatgaaa
    39301 atgctcttga gatcgatcct gatttcacag atgctatcag cggctgggct tattctttgt
    39361 ttgaaatgaa caagtttgaa gaatcagaag atttgtataa aaaagcactc aggacagatc
    39421 caaatgattc atatacatac tattattatg ctctgttaaa agaaaagatg aattcatatt
    39481 ctcaggctga tagtttatat aaaaaagctg tatcactgga tccaaaaaat tttgaagctg
    39541 caaatgatta tagtaatttt ttaattttac aaaacaggtt aaatgaagct agagttagta
    39601 taaaaaatat tttaaaggtc agacctgaca attctcaggc atattttaat ctgggtaatc
    39661 ttaattacaa tgaaaacaat tatgaagaat ctgtaaacaa ttttttaaaa tcatttagtc
    39721 tggattctac aaatgttgca gctcttttaa atgcttccag gatattaaat gaaaatgaca
    39781 gaaatgaaga ggcaattgat ttagttaaaa atattgatct gaaaaaaaat tatgattttc
    39841 aaactacaaa ggatattggc attttacttg aaaaccttga aaagaaatct gaaagtattg
    39901 aattttataa aagatcaatt aagcaatttc caaatgactc tgcttatttt agaaaccgaa
    39961 tggtaaattg aatttcttta atttagaaat tgtataattt tacagcaaaa ttaaattaaa
    40021 ataatctcat ttttttctaa aaacataatt taataaatct cctttctatt cattttttaa
    40081 acattcattg aaatttaaag taaaatcgta cacttataat ttctaaaact cagttttttt
    40141 ttctttggaa aacaaaaaca aaaataatat ttttgtttaa aggcgattaa gaagatgcaa
    40201 tacaatttta agtttttagt ttacccccgc aatgttcact ataatgcatc ttcttcgcct
    40261 gaaagtgctt attgaattat cgggggtttt atattttaat caaaataacg taactttact
    40321 ttaaaaaatt aatttactaa tttaaacaca ttaaatttca caaaaggaga tgaaatgaaa
    40381 tcaatctatt tagctctatc tttattctta gctttattta tatcaagctg cagcagtgat
    40441 tcaagttcag gtaataaccc gtttgtaccc ggaggaggaa ccggtacaac tgttacattt
    40501 acaactacct atgatgcaaa taatactcaa tttgtgtttg ctccaaatac cggtgtaact
    40561 atatcaaaga taacagcaaa ttgtgcagct gctaatatta acaatgaaga aattacaggt
    40621 gatggaacaa ctgtttggac acaggcagca ccggcagctg taagcactca aggcgttaat
    40681 gttgctgcag gtcagcaatg gactttcaaa atagaaggga aagtcggaag tgcaacagga
    40741 gaagtttata cttctaatac aagtattaca gtacaataat aaattttgca ataagtatag
    40801 aaatttgtaa aaaagaaatt aaattctttt aataaactaa aaaaaaaatg aaaaaattaa
    40861 acaacattgc tttattaatc attgttttat taatagtgtt tgcaagtaac aattccaatg
    40921 cacaggtaaa gattgcatta ggtttacaag ctggcgtaaa ttttggaaac gcttcaaata
    40981 atgtagctgg cgtagatcaa acattaagct caagtaccgg atttattgcc ggtggattag
    41041 tagatattgg tctttcaaag cagatatcca ttgtaccggg tctaaggtat attggtaaag
    41101 gttcaaagca aactaatgtt gctgtccagg gattaggtaa tggtgatctt aaaacaacaa
    41161 ttggaatgct tgaatttcct gcacttttga gagttaaatt tccattgaca gaggtaaacc
    41221 cttatcttat tgcaggtccg atactgggta taattctttc agccaatact gattttattc
    41281 caacagctgg cggaaccaca caaagttttg atgtaaagag tacatttgaa tctacagaat
    41341 ttgaccttta ttttggagga ggactggatt ttaaaatttc accttccgca agtctgtttt
    41401 ttgaacttgg atatgagtta ggtttgacag atattgctaa ggcttcaaat gtaaccacga
    41461 aatcttatag tttccagatc acaggcggtg caagatttgg aatgtaaaat taaattttaa
    41521 taattcaagc cggctgagta atctgccggc tttttttatg tgtaaaaatt ttccctccga
    41581 gtattaaaaa attcctttaa tgcaatcaac ataatcacta tatttgtagc ttaactaaaa
    41641 aattttatat atcaatgtct gctaaacatc atcaggaaga taaattaatg tcagcttatt
    41701 ataaagctgt agattttttc gaaaaaaata aaaaacacgt ttatactgca cttaccatac
    41761 ttatagtttt aattgcagga actgtacttt ttgtaaataa aagaacagct aataatgaac
    41821 ttgctgctgt cgaaattgaa aaggtaaaac ctttgtataa tgcaggtaat tttgaacagg
    41881 ctgtaaacgg tgattctctg ggtaattcca aggggcttat ttatattgta aatgaattcg
    41941 gatcaactga gaacggagaa tatgcgaagc ttatgcttgc aaattcttat tattccttaa
    42001 gggattttga taatgcagag aagtattata aagaattttc aggcagtaat gatgttctta
    42061 aagtgtctgc aattgcaggt cttgccggtg tgtatgaagc caaaaatgat tacttaaatg
    42121 cagccaggca atttcaaaaa gccgctaaca cagataaaga aaatccgttc gtcgatcagt
    42181 atttatttta tgctgctaaa gattatttca gagcaggaaa ttttgatgaa tcaaaaaagc
    42241 ttttggatga acttaaaaaa gattatccta aatcaaagta cattgcagaa tctgaaagat
    42301 tcagagcaag catgaaaaac tgatcagtgt atttattatc tgaatagttt ttaaaattat
    42361 gaataaaatt taatgagctt aaaaaataaa attaacgaag atctcaatac tgctctcaaa
    42421 gcaaaagaaa ctgtaaggat cgaaacactt cgttccatac gtgcagagat attgaaaatg
    42481 gataagtcag gtatgaacag ggaaatgaat gaagatgaag aaattcagct tctatccaaa
    42541 caggcaaaaa tgagaaaaga atcgatcgat cttttcagag aagccgcaag aattgatctt
    42601 gtcgataaag aacaggctca gcttgatatc ataaatgaat atcttcctaa acaaatgtcc
    42661 gctgatgaag caggcatcat cgttgataat atcctaaagg aatccggaat tacagaacaa
    42721 aaagattttg gaaaactaat gggagaagta atgaagcaac ttaagggaaa gatcgacggg
    42781 aaaataattc aggatcttgt caaaagcaaa cttaactgac ttttcctaga tcacactccc
    42841 ctcccgattt gaccacttta aattaacaat gtatttaatt ttttaagtat ttgaattttt
    42901 acagaattga tcaaaaaagc ttttgaatac tctcgacctc ataatttcaa tcactatcct
    42961 cattctttct ttactcggtc ttcagaaagg atttcttaaa agcgtatttt cactaatcgg
    43021 tattgttgcc ggatttttac ttgctacaaa ttataacgaa gttctgacgg gttatctttc
    43081 ttttataaaa atcaacgaga gaattttaag ccttatctct ttcttactga ttgttgtcat
    43141 tatcaatatc attctgaatt atgtttcaca aaagatctca ggatttaatt ttgcaacaaa
    43201 aactattgac aaactattcg gatttttgtt tggaatgttt aaaggaatta tacttacgag
    43261 tctatttctg cttgcaacta ctaaactatt ttctgcattc tcaaaaaaaa ctattgatga
    43321 ttcgaagata tatccttacg ttatagatgc agctcccgga atttacaata atattatgac
    43381 agtactgcca aatgcaaaaa acttctatca ggaatttgac ttcctgaagg atttcaataa
    43441 taaaaaataa atgaaatctg attaaaattg ataactcaga tcccgatgat ttcatgaaaa
    43501 tttgaaaagt actttttaaa ataacattga aattttaact aatataattg agtctaattt
    43561 tctttcaaaa cccctaccag ccctcacctt accaagggga aggctgggat gaggtaaatt
    43621 gaaggtagaa ttgaatttta atatttaaaa tatattttat tagaatttat tcttttaata
    43681 aattatcttg aacagcattg atataaatca aacaataaaa tggatttaac agacataaaa
    43741 tataaattag aatttgatag gatcacttct cgtctgaaga atttcgttta ttctgatctc
    43801 ggagaagaac tctgcgactc aatgaaattt ttttctgagt caggtgaact ggaatatgaa
    43861 cttgaaaaag ta
//
LOCUS       DAORVQ010000007        42202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_263_length_42202_cov_6.391867, whole genome shotgun sequence.
ACCESSION   DAORVQ010000007 DAORVQ010000000
VERSION     DAORVQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 42202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42202
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_42202_cov_6.391867"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLX80_01345"
     CDS             <1..642
                     /locus_tag="PLX80_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY19469.1"
                     /translation="RMFEKDGIEKFIGAGVYYGAAMTEAALYKDQDVCVLGGANSAGQ
                     GALFFSRYAKSVTIIIRADSLGKAMSSYLVERINATQNITVLTDTEVDSVSGSNKLEK
                     IFLRCKNEDKIKEMDTSAIFIFIGAAPRTEICKGIIETDEKGFILTGPDLLNKKNVTN
                     EWTLERDPFLLETNIPGVFAAGDIRSGSGKRVAAAVGEGSAAIGMVHKYLETV"
     gene            complement(944..3055)
                     /locus_tag="PLX80_01350"
     CDS             complement(944..3055)
                     /locus_tag="PLX80_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQY19470.1"
                     /translation="MTRKLFGRLCLTAIMYLMYSAATSPLFSQDTLKTGDTTDLRNFY
                     REEMLLESFDPEAEEPAVLDNLENLRRNPLDLNMITYEELDLIPFVSSVIARKIIDYR
                     NEIGKFKSKRELLKIEGMSIELYDMIKIYIIVRRSKTDVIIDETGIRYNTSEITFFDK
                     LNTRVRSRFLQDLQTKAGYISQKYEGTKPKLYTSVNSKFGKSGKTINSDKYLEINLTL
                     EKDPGEKYINDFTSGYIEGGGFGILKKFIAGDYSLNFGQGLTLSNSSYFSKGIDAVSP
                     LKRKGKGIDGYSSVNESQFFRGASVSLEQNNFYLDLFYSYNYLDAAVDEFSEEASTIY
                     IDGYHRTSSEINRKNSLKENLFGGRFSYTKGLLRTGITYWSGKYSRSVIKDTVRELYD
                     FSGDKASSLGGDYDVLYKNMNAFGEWTYSNSGAVASINSVSFSFPGLVQLVMSYRNYP
                     YDFISVHSSGFGERGGETRNERGFYTGLTLKPLKGLVLNAYFDQFKIPYRTYLNPLPV
                     SGNDLLFNADWRAQKGFVINLKIKSETKEDLQTIQNLNLQDIKITDRRSQFNARLGFI
                     YQLTDALRFRSRFEFVNVKYDMSGASSKGRLFYSDMRLMPFRGIIFDMRIIYFDTENY
                     DSRIYEYENDIKGIMTNIALYGKGRRWYAVMRYKPFPLFEISAKYSETYTDGVKTIGS
                     GNDMIEGDIVNRLNVGVEINF"
     gene            complement(3271..3615)
                     /locus_tag="PLX80_01355"
     CDS             complement(3271..3615)
                     /locus_tag="PLX80_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HQY19471.1"
                     /translation="MAYNEKTSKRISNALMKKGIEFEEKKMFGGIAFMIKGKMSVGVM
                     KDDLTVRVIEEKYEKALSDQAAREMDFTGRPLKGFLYVNESGFKTEKALSKWIDLGID
                     FVNCLKINKKKK"
     gene            complement(3768..3908)
                     /locus_tag="PLX80_01360"
     CDS             complement(3768..3908)
                     /locus_tag="PLX80_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19472.1"
                     /translation="MAQVNSFNKRFPSALYGKDSGWEDVLAGMNPKIDLDTLLKIQIM
                     KT"
     gene            4247..4789
                     /locus_tag="PLX80_01365"
     CDS             4247..4789
                     /locus_tag="PLX80_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3'-deoxyribonucleotidase"
                     /protein_id="HQY19473.1"
                     /translation="MRLLIDMDGVIADFEGDFLKRWKDLHPEKNYVPLEERKGFYVKE
                     QYEEDHRDHVQEIYLSPGFYQNLPPIPGALEALREIKNSLNNVFLCTSPMLPKYENCV
                     LEKFHWVYEHLGTEWVNNIVLTRDKTIVRGDVLIDDKPDIKGSAEPEWEHVIYDQPYN
                     RNIKDKRRLTWQNWKEVLGI"
     gene            6623..7051
                     /locus_tag="PLX80_01370"
     CDS             6623..7051
                     /locus_tag="PLX80_01370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY19474.1"
                     /translation="MDGKKNIVIKTNKGNIRIELFPDAAPLTVLNFLKLSEKNYFDGT
                     IFHRVVPNFVIQGGDPTGTGYGGPGYSIRSEFSPVDFGTGYLGMASSGKDTESSQFFI
                     THSATPHLDGRYTVFGKVTEGLDVVDIIMIGDVVEDVVVE"
     gene            7497..7982
                     /locus_tag="PLX80_01375"
     CDS             7497..7982
                     /locus_tag="PLX80_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY19475.1"
                     /translation="MNLIREKKHRLEKEFYIGVKTVSFTACIKKEEKVFTNNFIFSIF
                     RDKLIESIKKYGCEALIYLFMPDHLHLILKGKNNDSNVIKAIDLFKQKTGYWFSQNML
                     YSKWQKDYYDHILRDNEDLRSHIYYILNNPVRAGIVSNWKDYEFKGSTVFDFDKWDLN
                     Q"
     assembly_gap    8258..8357
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8358..8729)
                     /locus_tag="PLX80_01380"
     CDS             complement(<8358..8729)
                     /locus_tag="PLX80_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQY19476.1"
                     /translation="MHEIKWNYDSSLTFRRSCAHGICGSDGMKVNGKNKLAYALLLKY
                     INTKKPIVIDPLPALPIIKDLVVEMTDFFDKYEVIKPYLINNSPPPPDIERLQSNEDA
                     EKLFESAKCILCACCTTSCPST"
     gene            complement(8865..9620)
                     /locus_tag="PLX80_01385"
     CDS             complement(8865..9620)
                     /locus_tag="PLX80_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQY19477.1"
                     /translation="MLCVIFVLFFNERNFAQVKDLNLKFGKNIFKTKFNSEEYTTTLS
                     IYREDEKIFEEIYYDLIPEIARMDLDNDGKKEYLIAMYSGGAHCCTSLFTGKISKGKF
                     NFTDTIIWGNSFFKAEDLNADGKTEIVGFNDVYAYMFTNYAQSKFPLLIYGYENKKFV
                     NVTAKFPKQINDNLTELKKELDEYKNFKCEEAGTETFNTDAGAVKAILAAATEDYMLL
                     GKVDEGYKLIDEFYKCPDRDGFKDSLRINFLLK"
     gene            complement(9656..10927)
                     /locus_tag="PLX80_01390"
     CDS             complement(9656..10927)
                     /locus_tag="PLX80_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY19478.1"
                     /translation="MIKKFDVVIVGAVGAGLRAAVEIPKEYSCAVISKVFPSRSHTGT
                     AQGGVCAALANMEEDSWENHAFDTVKGSDYLGDQDCIEVLCKDAIRAIIEHEHMGMPF
                     SRNEDGKIAQRQFGGHTKPADKNDPYGKRLPIKRACYSADRTGHVMLQTLFDNTIKNQ
                     VTFFSEYFVTELLTKDGVCKGVVAFDIATSEIHIFQAKAVLFATGGNGRICRITSNAH
                     VGTGDGMTLAYRAGLPLEDMEFYQFHPTGLWKLGILVSEAARGEGGILKNKDGERFMA
                     RYAPTVMDLAPKEKIVSIRTELQNTMMEDCGVFRTEEGLRKMIEKLKELRERYKNIAV
                     QDKSKVFNTDLVEALELDNLLLNAEATVYGAYARQESRGAHTREDFPERDDQNWMKHT
                     FAFSRGQEVPDLKFKPVIVTRFQPMEKKVLI"
     gene            complement(10976..12631)
                     /locus_tag="PLX80_01395"
     CDS             complement(10976..12631)
                     /locus_tag="PLX80_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQY19479.1"
                     /translation="MILNIIKENKFFDLRYFLNLLKLLTSVFIIFISCENVSAQDKTV
                     SPDNYLSDDDFNAEQIIDNNKESLISIWKSQTDYYSYGEDKYIDTSLLNGSGFILNEE
                     GLIGTNNHVIEGIDSLIIKTSNGNFFYAEVVMSDAEKDFALIRIVNSNGITFKPVKLG
                     NSDDLKAGQEVFAIGSPLGFEYTISSGIVAAVRENEKVNFGDPYSYTGNEKLFEKVIQ
                     ITAAISPGNSGGALFNRKGEVIGITTYTYLGYGNLNFAIAINSFKKFMDFALLNESEI
                     TDDMLIKKEENIFNTTFKNAVNQKWELAADWYYTKLKDSLDSKDEFTLRQDSINKVRF
                     VKVEELFNNCLTLQPDSFFVYEELLDLYVNTDNPEKTEVLYETILKRFDSDSLLNLIS
                     SKLAAAYSSTGNYEKALKFYYKMLDEDSRLYYVNFQIAAIYEEKKDYKNAVAEYKNLL
                     VKDPTYIQAYTRLGKIYYEQIKDINKAKYYLEKGYNKELEQTGYTTYDTDMLYYLGMI
                     AVKEEKKLEAILYFMDLKSNAGYYEDAVKKKTKLLKEIKKLDE"
     gene            complement(12628..13440)
                     /locus_tag="PLX80_01400"
     CDS             complement(12628..13440)
                     /locus_tag="PLX80_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY19480.1"
                     /translation="MSDKSHLLGKFNNILVSEIENPKGLKVGLIQLNRPDVLNALNND
                     LMKEVVETLEAFDKDSETGCMVLTGNAKAFAAGADIKEMAEATAIEMLIRDQFARWDR
                     IRKIKKPVIAAVSGFALGGGCELAMSCDMIVASKSAKFGQPEINLAVIPGAGGTQRLT
                     KAIGKARAMEMILTGKMYTADEMYDAGLVTKVVENEIYLDEALELAKEISSKPAIAVQ
                     LAKECILKSFDSTIEAGLEFERKNFYLLFATEDKFEGMKAFVEKRKAEWKGK"
     gene            13609..13803
                     /locus_tag="PLX80_01405"
     CDS             13609..13803
                     /locus_tag="PLX80_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQY19481.1"
                     /translation="MDQLVIPPKVRKHFGIKSGTVIHFIYSNGEIKIVQVTKETISTN
                     FGIFGTKGKVLKALKKEKKI"
     gene            14006..14218
                     /locus_tag="PLX80_01410"
     CDS             14006..14218
                     /locus_tag="PLX80_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19482.1"
                     /translation="MYKSIISNYPIELINSDKELRLNAANIKSKVKLSYAEAFAAGLA
                     KMLDATLLTGDKEFEPLSDKINVEWL"
     gene            complement(14285..14782)
                     /locus_tag="PLX80_01415"
     CDS             complement(14285..14782)
                     /locus_tag="PLX80_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQY19483.1"
                     /translation="MTEENFNKESFSHGILLKASPEKVYEYISTGSGLSKWFIGSADY
                     FYNDKSIRLGDEPAQKGNSFLWKWLKKDLELKGIITESDGKNEFGFTFGSSFFVNIKL
                     SESGDRTKLVLTQCYQDSATENEFGFINCCVCWVFFLTNLKSVLEHGIDLRETEADIE
                     MLVNR"
     gene            complement(14792..15133)
                     /locus_tag="PLX80_01420"
     CDS             complement(14792..15133)
                     /locus_tag="PLX80_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HQY19484.1"
                     /translation="MIIGVAGPFSADTEAARKANLDAMNEAAAKILELGHIPLIGVNA
                     ALPVIEKSNIENKYEAIMEISMAVIDRCEAILMIGSSPGADRERDFIKSKGLPVYYSI
                     DEIPIIKNKIC"
     gene            complement(15325..15723)
                     /locus_tag="PLX80_01425"
     CDS             complement(15325..15723)
                     /locus_tag="PLX80_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19485.1"
                     /translation="MKVDLGRAVILVEDYDEAFEFYKDNFGCKKFFDITNDDGERFLH
                     ISFDSETRAGVWFLKANTTEQQSLVGRQTGGAPVLVLYTDDFDVLNEKISDNGIEYKS
                     EPEITEEFKSVSVFDLYGNEIVIVQLLTTQ"
     gene            complement(15746..16423)
                     /locus_tag="PLX80_01430"
     CDS             complement(15746..16423)
                     /locus_tag="PLX80_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HQY19486.1"
                     /translation="MKIILTVFILNLFICTAISQSKYDTLYIANHKVSCIGVAPQECF
                     LIKNSESEEWKFHFNDIKGFEFIPGNEYIIEVEKLFVATQAADDASFYYNLINILKAE
                     PTMVISDTDRVKLDKRSYIMKGMKTEENFDRESDFSKVYIKFDLDENRINGRDGCNSI
                     NGKIKIDSGKIEFLPMITTKMMCENVKVFSSFHKNIHLINNFKLEEEKLMFYKDDEFL
                     MEFEIQN"
     gene            16590..17591
                     /locus_tag="PLX80_01435"
     CDS             16590..17591
                     /locus_tag="PLX80_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQY19487.1"
                     /translation="MKKKNVKETNSINIPTIMRIAYIDRQISMNNYPSAIKLSKEYEV
                     SSKTIIRTIEFMKSFYNAPIEYDKSIKGYFYSDKNFKLVKFDLNDEDLRSLALISNAF
                     KIYGGKPFSSDLNSFFNKIYYIYNDKYSIQLKDIENIISFRIRNSRSVDTKFFEIIKT
                     AIINSETLKCRYYSGYSGQTTDRELDPYHIVNDKGEWYLIAFCHRDNKIKLFSISRFK
                     EVHPSGKQFSPVDDFNVEKFFEDSFGVFNDDKKIKVRMRFEKRSAGYIKEKTWHRSQI
                     IKENKKDGSVILEMTVNSSDEIKHWVLSWGRSCTLLSPKTLVDEIKSDLKSTLKNYS"
     gene            complement(17967..21584)
                     /locus_tag="PLX80_01440"
     CDS             complement(17967..21584)
                     /locus_tag="PLX80_01440"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQY19488.1"
                     /translation="MKISRLLVNKGISAYDQIEFDSRSSVIRNPDGSIVFEMNDIKVP
                     DHWTQVATDILAQKYFRKAGVPVYMKKVEEEGVPGWLQRSEPDHEKLKDVIEEERYTS
                     ENDSRQVFNRLAGCWTYWGWKHNYFDHEEDAANFFEELCYMLAMQYAAPNSPQWFNTG
                     LNWAYGITGPSQGHFYVDPISGKLTTSKDAYTHPQPHACFIQSLSDDLVNDGGIMDLW
                     VREARLFKYGSGTGSNFSNVRGVNEPLSGGGRSSGLMSFLKIGDRSAGAIKSGGTTRR
                     AAKMVTLDMDHPDIEEYINWKGMEEQKVAALVTGSKLLNVYLNKIMKACHSVHPENDG
                     FDKNTNKQLAQAIHDARKIHIPENYIERVIQLGKLGYKEMEIPEYNTDWNSEAYLTVS
                     GQNSNNSVRVTNAFMESVVADGDWNTYWRTELKKASAENREPKPYKSYKSRDLWEQIA
                     YAAWSCADPGIQYHDIINDWHTCPEGGKIIASNPCSEYMFLDDTACNLASLNLMKFYN
                     NETTGFEIDRYRHASRLWTIVLEISVLMAQFPSKNIAKLSYEYRTLGLGYANLGALLM
                     VKGIPYDSPEALSITGALTAILHMTSYSTSAEMAKELGPFQRYKENEQNMLRVIRNHR
                     RAVYNVGASEYEDLSITPMGINKNHCPEPLIKAAIEDADKALELGEKHGFRNAQVTVI
                     APTGTIGLLMDCDTTGVEPDFALVKFKKLAGGGYFKIINQSVPPALRNLGYNEKEIDE
                     IIKYTKGHGSLIGCPHINAQSLSEKGFTKEIISKVEKSLPSVFDINFAFNKWTLGSGF
                     CKNALGFSEDQTDDPSFNILKELGFTKEQIEEANDFVCGTMTVEGAPYLKKEEYAIFD
                     CANKCGKKGTRYILADAHIKIMAAAQPFISGAISKTINLPDHATIEDMKNAYLNSWRL
                     GIKANALYRDGSKLSQPLNSIKDDDIIDYNNSVDINTEASRSHDIVKIAERIIHRYIA
                     KRRKLPFRRKGYTQKAKIGTHSVYLRTGEYENGQLGEIFIDMHKEGAAFRSLMNCFAI
                     AISLGLQHGVPLDEFVDAFVYTRFEPNGIVVGNPNIKMSTSIIDYIFRELAVTYLSRN
                     DLSHIENNTEYQRSPIDALSKLDNDTDFDEEEVYSERIIDEPGIDFNKPDKSTAAASN
                     KIKLAKTQEQSQTRSMEKIREAKLKGYTGDICQQCHSVTMVRNGTCLKCITCGETSGC
                     S"
     gene            21927..22745
                     /locus_tag="PLX80_01445"
     CDS             21927..22745
                     /locus_tag="PLX80_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQY19489.1"
                     /translation="MIKIFTVDAFTDRPFSGNPAAVCLPNVELSEEVMQKIAFEMNLS
                     ETAFVTKLDDGFSLRWFTPKAEVELCGHATLASAHILWQEKMISENEEAVFYSKYKGI
                     LKAKKSGSEITLNFPVNKPLPSDRNEILEKALGVNSVSLNTIFTTEHHYIVELNSEEN
                     LKNVKPDFSLLETLPKYGVIITSKSDDPEFDFVSRFFAPSKGVKEDPVTGSAHCVLAP
                     FWSEKLGKKKMNAFQASERGGVLSVELKDERVLISGNAVTVTAGELFLHTDSFS"
     gene            22854..23564
                     /locus_tag="PLX80_01450"
     CDS             22854..23564
                     /locus_tag="PLX80_01450"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HQY19490.1"
                     /translation="MLQRDYLMKMVEMLTAVMAKVIFNKEQKKYDEALTEIENAGKNI
                     IGLDIGMVKILKIDDFTGLMKTSDVFAGRCLISAELLLEYAKILEEKGTDTDLKNDFY
                     NKSFRLYTEALLTKEILEPTEYIEKAESILPVLSNSGYFNIQYDYYSNNLTLFEFYNL
                     SGNYSKAEDILFNILESGSELSSEDDEENKRKLRKRAVAFYNDLLSMDEEKLASGNFS
                     KDEAEDGLQEIMKMMKLN"
     gene            23601..24371
                     /locus_tag="PLX80_01455"
     CDS             23601..24371
                     /locus_tag="PLX80_01455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQY19491.1"
                     /translation="MKKIFLTLIVFFAFCGNSFSQSDTNTALSGLKYLILEEGDGPDA
                     ESGKAVEVHYTGYLLDGKKFDSSLDRNEPIMFVLGAGQVIKGWDEGISLMNVGDKMRL
                     IIPSELAYGNKGAGDIIPPDATLVFDVELVSVGDPKTSIAEVLLSTIVDVNVQAAISQ
                     YKELKANEPDIYNFKESELNKLGYQLFQIGKIPDALEILKLNVEMFPNSSNVYDSLGE
                     AYKVSGNNELAIVNYNKSLELNPENENAKNMLKELEGK"
     gene            complement(24675..24878)
                     /locus_tag="PLX80_01460"
     CDS             complement(24675..24878)
                     /locus_tag="PLX80_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQY19492.1"
                     /translation="MEKGTVKWFNATKGYGFITRADGQDVFVHFKAINSEGYKKLDEG
                     DEVEFEVEDGAKGPQAANVSKVM"
     gene            25223..26965
                     /gene="ptsP"
                     /locus_tag="PLX80_01465"
     CDS             25223..26965
                     /gene="ptsP"
                     /locus_tag="PLX80_01465"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQY19493.1"
                     /translation="MDKDIIYKGIPVSPGISVGEVYLYVRNNFEVNSRDLIPEEIEKE
                     LDDFQRSVEISVKELSKIRNYSIEKIGEVNSLIFDAQLEFLQDKFFLSQVIHRIQTEK
                     RTAGYIFNDEISKLEFALMDSKDTYLKERFEDIKDIKNRVLRNLKREKLVSKIEENLI
                     IVSHELTPADTILFSKRKVLGYAVDKGGNTSHAAIISRALRIPAVVGMKDITKSLRSG
                     DNIIIDGSAGIIIKNPSEDTLKEYDVKIIKLKEHEEKLKEIIDLPCVTLDGKKIELSA
                     NIDFDEEIDFIVNTGHCGIGLYRTEHLFLEEGGFPSEEKQLEEYSHISNVVYPKAVTI
                     RTYDIGGDKLLPRSEKEDNPFLGWRGIRICLDREDIFKEQLRALYRSSVNKNIRILIP
                     MISSLEEVRKTKLIIKEVIEECEKNDIPIDKNLKIGIMIEVPSASIMAEELAKEVDFF
                     SIGTNDLIQYLLAVDRGNELISSLYKEFHPAVIRTIKIIADAAHKNKIKISVCGEMAS
                     NPLAVPVFIGLGVDELSVSPSKFPEIKQIIRSLHSKKAYRLCKEILKLSSEMEIKEKV
                     ENFNEAITNEIKVI"
     gene            27069..28100
                     /gene="nuoH"
                     /locus_tag="PLX80_01470"
     CDS             27069..28100
                     /gene="nuoH"
                     /locus_tag="PLX80_01470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQY19494.1"
                     /translation="MPEYLIELIITLSKISVVLAVLLTGVAYSVYAERRVSAFIQNRY
                     GPNRVGPQGLLQPLVDVIKLLLKEDIVPEKANKFIHSLAPVISIIVSLSTFAVIPFGD
                     TITIGDRVIKLQIADVNIGVLYFLALTSLGVYGVTLSGWSSNSKYSLLGGLRSAAQMI
                     SYELAMGLSVIAVILMNGSLQLDTIVSNQAGWKWNIILQPVGFIIFLTSAFAETNRTP
                     FDLPEAEQELVGGYHTEYSSMKFALFFLAEYANVIISSAMIATIYLGGWQFPYLQDFG
                     LPPMAVSILQILTFVVKVVALVFFFIWVRWTIPRFRYDQLMNLGWKVMLPLGIANLVI
                     TAIVILLMK"
     gene            28130..28639
                     /locus_tag="PLX80_01475"
     CDS             28130..28639
                     /locus_tag="PLX80_01475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQY19495.1"
                     /translation="MKKIILTILLVFVFAFNLSAQTYNNFTLEDLDNNEITLDDLIKK
                     GPVLISFWALWCGPCKEEMKKMQPIYEKYKDQGFTYLAINQDNQKSVSKVKSFINANK
                     YTFPVVLDTENDVFEAYNGIGMPYSILIDMNKNIAAKHLGYVTGDDKIIEQEIKEVLM
                     AGSNYKGDK"
     gene            28678..30336
                     /locus_tag="PLX80_01480"
     CDS             28678..30336
                     /locus_tag="PLX80_01480"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HQY19496.1"
                     /translation="MIRKVFIISLFVCCANISFGQINLDVDASVSNLLRYGNGYEYSG
                     NVRNAKEYFENLTDARLKVNGIVFGLRYNVSNPIEYGQNFVGIDKRYIQYEHDIGVSL
                     RAGDFYEIIYRGLSLNVFEDRPLAYDTGIDGVRVTYNHNFGSKKNPFKFEAQVLGGDI
                     KYIDYLDPDREERYKVRGVFTEFSPIKPLTVGFNFIYSIGELPEQDLITTVTSNVPEL
                     SATYTTQDFEFYAGYAFKSSIITPNEIYPDPNTALGDGVYSSLSYSFNNIGLTLDYKN
                     YRFDVTLPDNRSNTRPSRMLPYQNPPTAIREHTSVLISRNPHVVDFNDEVGGQIDLVY
                     APNDKLSFNLNGSIASRHYQYTDTDSGATVSYERVDRGDSFLPDLDDAFSPFWEFYLE
                     GENYATEDLYYRLAFARQSSVLYNQLNPNSSEKLLTSTVPAEFKYSFYKNLTFKINAE
                     MQWVHNSIRVSEDKNFTNQYFALSFIRAPDITLTVGSEFTSDEEEPTGKSSWFIGEAT
                     YQINQSNTVGVGFGTERGGLKCANGICRFVNPFSGFRLSVATQF"
     gene            30381..31496
                     /locus_tag="PLX80_01485"
     CDS             30381..31496
                     /locus_tag="PLX80_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19497.1"
                     /translation="MSLKKFSSSLIFLSLIIFSGLLFQSCESNDSPTTNNTPASTVPV
                     PQSPENNSTVSTLTPLFNWAGVNVAETYKLQVSENNTFTSIVLDVDGITSTFFSSVGS
                     SLQDSTTYYWRVKGLNTSDTTDWSIVYGFAISLESINPTTKVLVELFTNTSCIPCVEA
                     NQYLDGINSNLGVTINDPNVLILRVHTTLYAGDPFYLYNTADNNARMIVYPGTPTSNP
                     RGYLLGAFMNNYSSSAWTNVLNQKLSETRSFAVNLTNVYDSVSRNGNITVKIKQVSGP
                     TYNDLVYHIALSESEIQYNAPNGETEFENTFRDLVTPPSGQPMNIATGQTNSYDHTYT
                     VDSAIDQDHAYITVFAQRTTAVSGIREVMGAERVKIK"
     gene            31773..32570
                     /locus_tag="PLX80_01490"
     CDS             31773..32570
                     /locus_tag="PLX80_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19498.1"
                     /translation="MFRKFTVFLLFTFFISTYSYSQKVETSAEKLAIELANPVADLIS
                     VPFQNNTVWGIGEYNGSQNVLNFQPVIPIAISKNINIINRIIVPMVTQYNITGPGQNQ
                     NSLGDIVYSIFISPNSKKLIWGIGPVMSIPTATNKLTGSGRFSVGPTGVILKQSNGWT
                     FGALINQYWSVGGNPDRGNQTVGYLQPFLNYNWKGGGGISMNAEMTQNWKIKRTQTFM
                     NILITGITKLGKFPVSLGVGPRIPITASPEAKGFFGIRAVATFIFPK"
     gene            32837..33829
                     /gene="nadA"
                     /locus_tag="PLX80_01495"
     CDS             32837..33829
                     /gene="nadA"
                     /locus_tag="PLX80_01495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQY19499.1"
                     /translation="MENILKDKTVSRLKIDGVRPIDLSLDLREEISKLKSEMNAVILA
                     HYYQESEIQDLADFIGDSLQLSQQAAKTEADVIVFAGVHFMAETAKILNPDKLVILPD
                     LNAGCSLAEGCPAPMLMKFKEKYPDHIVISYINCSAEVKAISDIICTSSNAVKIIDQI
                     PVEQKIIFAPDRNLGRYVIKKSGRDMVLWQGACIVHETFSEKRINKLKFENPDALLIA
                     HPECEEAVLSNADFIGSTSGLLNFVTESEKTEFIVATEPGIIHQMLLKNPSKKFIPAP
                     SESGCNCNECPYMKLNTLEKLYLCMRDRKPEIVMDEDILKKALLPLERMLEMSK"
     gene            complement(34039..34932)
                     /locus_tag="PLX80_01500"
     CDS             complement(34039..34932)
                     /locus_tag="PLX80_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HQY19500.1"
                     /translation="MLKEFDIDINKYFAKPDKKVDLSKWDTEYKGDKLDKESSLEILE
                     SGKEKLSAIQDVLYADNKYSVLIIFQAMDAAGKDGSIKHIMSGFNPQGVIVHSYKAPN
                     TLELEHDYLWRHYIDLPRAGNITIFNRSHYENVLVTRVHPEYVMNEKIPGINSVKDID
                     DNFWSQRFEQINHFEKTVRQNGTVIIKFYLHLSKEEQKKRFFKRIDKPEKNWKFSMND
                     VNERKYWDDYQKAYEEAISNTSKENSPWYIIPADNKWYTRILITAVIHKYLSNLDLKY
                     PEVNEDMKKELISAKEELLKE"
     gene            35159..36001
                     /locus_tag="PLX80_01505"
     CDS             35159..36001
                     /locus_tag="PLX80_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19501.1"
                     /translation="MKKTLLILFISLTPVINLYSQENPQQVYRNGIEKFFNSDFDLAI
                     TYFTDYVKTETQAYEAYNYRGLCYLAQKNYAKAKEDFSTAVHLNIKYSQGFVNRANIF
                     ALEGNYEAALRDYNNAILADRANLEIYYGLSLLYINSKEFNKALIEMKTAAAIDPKNA
                     RVYMNTAIVNMLIGDTLKIFENVDEALYWDSSYLYTNINKSLIFTIADRFQYAIQVLT
                     NVISSNPNSYLAYFNRGVVFYMMGNFSEAISNFNRSKDLYKGGDQRIINLINKIVRIS
                     EFNS"
     gene            36105..36947
                     /locus_tag="PLX80_01510"
     CDS             36105..36947
                     /locus_tag="PLX80_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19502.1"
                     /translation="MKKILLLLIISISTLTNLYSQENPQSIYRNGIEKYYNSDFDEAI
                     VYFTDYLKIETQAYEAFNYRGLCYLAQKNYQNAKNDFTSAVHLNKSFSQGYVNRANIN
                     ALEGDYDAALRDYRDAEEVDRANLEIYYGLGLLFMNSKEFNKAEIELKKAAAIDPKNS
                     RVYMNKAIVNMLLGDTLNIFENVDQSLYWDSTYLLADLNKSLIFTIADRFKNVVEILT
                     EKISDYPNSYLAYFNRGVVYYMIGNYTNAIQDFERSKILYKGKDQRIINLIDKILRIS
                     KFNS"
     gene            37026..38198
                     /locus_tag="PLX80_01515"
     CDS             37026..38198
                     /locus_tag="PLX80_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HQY19503.1"
                     /translation="MRKPGQIIALGGGMFSMESDNGMLDKYILNLVPKEKPKICFMGT
                     ASNDGIEYRDMFYNFFNKMKCEPSHLSFLDQPKNIEKFILSQDIIHVGGGNTSLIIDT
                     WKKFGVDKIMRKAWRSGVILTGMSAGAICWFQDGITNPSPGKLNRLPCLGLLKGSFCP
                     HYDDKVELKRSFHKLILDGVIADGIGAEDGAAVHYLDDKIIRVVTSRPGVTAYSVKKV
                     KNKIVETALDSVYLGKESDIEHVQNNSPIEIFTTAISYIEKINEHDIKGICELMSDDH
                     IFIDSLGMVIKGKKEMSNAWKMFLKWFPDYNITIMNTVLTDDTVGIFGIASGTFNSDD
                     IDISDNFEIPGAWRAKVKDKQITEWQAIADNEAVRDIIKSNGVKSMFRSKMKSKSF"
     gene            38261..39430
                     /locus_tag="PLX80_01520"
     CDS             38261..39430
                     /locus_tag="PLX80_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQY19504.1"
                     /translation="MTEKENFAKELKRSLDEDTFVKIQFGKYRGDDKDFQKIIVTKLI
                     LKEGEHLSFNFKYNSKDVVKNFEIEKGISLADEITGKDFLSATLFTTENDFTIDHSKK
                     RVASFHKKKPSLKNPAVSVHNREKARNIDPEAKYFYLLGITTEEGKVKSGMYDKFKQV
                     DKFIETMDSLFRASALSEKDEIIITDMGSGKSYLTFALYDHFKNTLNKKVNIRGIEQR
                     QELTDLSNGIAKKCGFNENNGLEFLTGSIEEADLNNTNIAVALHACDTATDDAIKKAV
                     RAKAEIIVLAPCCQKYVRKKIVVPERLKGIFGYGIHEERLAVMLTDGLRAMTLENLGY
                     DVKVFEFISSEHTARNTMITAVKNELKDIGNSKDIESVKKEFGIDDFYLDKILNA"
     gene            39449..41233
                     /gene="carB"
                     /locus_tag="PLX80_01525"
     CDS             39449..41233
                     /gene="carB"
                     /locus_tag="PLX80_01525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQY19505.1"
                     /translation="MKKINREIILKAKKNGFSDKQLSVMLGTSEDNIRKYRFANDIIP
                     VYKTVDTCAAEFETSTPYHYSTYEKYNENFSSDKEKIIILGGGPNRIGQGIEFDYCCV
                     RCVKALREEGFETIMINCNPETVSTDYDITDKLFFEPLTTEDVLNIVHYEKNVKGIVV
                     SFGGQTPLKISRELEKNGLKILGTSSKNIDIAEDREKFGKLLDKLSIPKPEYGTCTNT
                     ETALKTANRIGYPVLLRPSYVLGGRAMQIVYSDGQIEKFFRDEAEISKEHPVLIDKFL
                     EEAREIDVDAICDGEDVYIVGIMQHIEEAGIHSGDSTSILPPISISKKNLDEIKKYTK
                     KLALALNVIGLINIQFAVKDKIVYILEANPRASRTVPFVSKTTGIPVVMIAAKIISGK
                     KLKDFKLKSFEEIEYIGVKESIFPFQKFPKVKMFLGPEMRSTGEVMGIGATFGEALAK
                     AQESTGAPVPTRGNIFISVNENDKKPKTIEIIKEYTKLGFGVIATSGTSVFLNENGIK
                     SEAVFKVNEGRPNVVDRIKNGEIQIVINTPLGADSRFDEYAIGWTAVQQKIPFITTLS
                     AAQSFAEGISRIKRGKTSVKSLQNYYKK"
     gene            41292..41477
                     /locus_tag="PLX80_01530"
     CDS             41292..41477
                     /locus_tag="PLX80_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19506.1"
                     /translation="MDLEKTKNSYKWDPASGDYSKSYVSYERKISNKKQSRKSLLTII
                     FILAIIVSIVVIFIEFS"
     gene            complement(41682..>42202)
                     /locus_tag="PLX80_01535"
     CDS             complement(41682..>42202)
                     /locus_tag="PLX80_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY19507.1"
                     /translation="KYKSLAGANGQFILWKKETYEKSGGHSAVKNEIVEDIEIAKYLK
                     KNGYNTLLLLSNDLVFCRMYNSFKEAVSGFSKNIYPASASSPLLFIFNILFIVLSNIL
                     PLILMFYNEYYIGLILIIILQRVIVSIVSSQNIFMNILLYPLQMLMLLYISIRSVVHS
                     RSGRSVWKGRTL"
BASE COUNT        13973 a         7235 c         7191 g        13703 t
ORIGIN      
        1 cgaatgttcg aaaaagatgg cattgaaaaa tttattggtg caggtgttta ctacggagct
       61 gcaatgactg aagctgctct gtataaagat caggatgtat gcgtcctcgg cggtgctaac
      121 tctgcaggtc agggcgcatt gtttttttcg agatatgcta aatcagttac tattataatc
      181 agagctgatt cacttggtaa agcaatgtcg agttatctgg ttgaaagaat taatgcaaca
      241 caaaatatta cagtattaac tgatacggaa gttgattctg tatccggcag taataaactt
      301 gaaaaaatat ttcttcggtg taaaaatgaa gataagatca aagagatgga tacttctgct
      361 atatttattt ttattggggc tgctcccaga acagaaatat gcaaaggaat tattgagacc
      421 gatgagaagg gatttatttt aactggtccg gatctgctta ataaaaaaaa tgtaacaaac
      481 gaatggacac ttgaacgtga cccatttctt cttgaaacaa acatacccgg agtttttgct
      541 gccggtgata tccggtcagg ttcaggtaag cgtgtagcag ctgctgtcgg tgaaggatcg
      601 gctgcaattg gtatggttca taaatatctt gaaactgtct gaaaaaatgt tcgcccgtat
      661 ccatactccg gtttagatac gccaatgaac ttgaaacacc gtttcaatca ctcctcctgc
      721 catttatacc tgcagattac ttttagagcc tgaccccgca tgcttcacgc tgtccgaaaa
      781 caaaaattaa agtttgctta aaaggttgct tccaagactc gaatgctcaa agaagatttt
      841 ttttaaagtt ttttaaactt agggtcttag agcctttgag gcaattccaa cgttttcgga
      901 cagtctgctt tcagtagaga gtcgttcata ttggactcac gtctcaaaaa ttaatttcca
      961 ctccaacatt taacctgtta actatatcac cttctatcat gtcatttccg ctgccgatgg
     1021 ttttcactcc gtctgtataa gtctctgaat actttgctga tatttcaaaa agcggaaatg
     1081 gtttataccg catgactgca taccatcttc tgccttttcc ataaagagct atatttgtca
     1141 tgatcccttt tatatcattt tcatattcat aaattctgct gtcataattt tctgtatcaa
     1201 aataaattat tctcatgtca aagataattc cccgaaaagg catcagtctc atatcagaat
     1261 aaaacaatct tcctttacta ctcgctccgg acatatcata cttcacattt acaaattcaa
     1321 atctgctcct gaatctaagc gcatctgtaa gctgataaat aaaacccagt cttgcattga
     1381 actgacttct tctgtcggtg atctttatat cctgaagatt taaattctgt atggtctgta
     1441 agtcttcttt tgtctcactc ttaattttca gattaattac aaatcctttt tgtgcacgcc
     1501 agtccgcgtt gaaaagaaga tcatttcccg aaacaggcag cggatttaaa tacgtcctgt
     1561 acggaatttt gaactgatca aaatacgcat tcaatacaag tcctttcaaa ggcttcagag
     1621 tcagcccggt ataaaatcct ctttcatttc tcgtctctcc gcctctttca ccgaatccgg
     1681 atgaatggac agaaataaaa tcatatggat aatttctgta tgacatgaca agctgaacca
     1741 atccgggaaa tgaaaatgat actgaattga tcgatgcaac tgcaccagaa tttgaataag
     1801 tccattcgcc gaatgcattc atatttttat aaagtacatc ataatcacct ccaagtgaag
     1861 atgctttatc tccgctgaaa tcgtataatt ccctgactgt atctttaatt acagaccttg
     1921 aatattttcc cgaccagtaa gtaatgccag ttctcagcag acctttggtg taagaaaatc
     1981 ttccgccaaa taaattttct ttaagtgagt ttttcctgtt tatctcagaa gaagtcctgt
     2041 gatatccgtc gatataaatg gtagatgctt cttctgaaaa ttcatctaca gcagcatcta
     2101 aataattata agaataaaac agatccagat aaaaattatt ctgctcaaga cttacagatg
     2161 cgcctctgaa aaattgagat tcattcacag aggaataacc gtctattccc ttaccttttc
     2221 ttttcagcgg actcactgcg tcaatacctt ttgagaaata gctggagttt gacaaggtaa
     2281 gtccctgtcc gaaatttaaa gaataatccc ctgcaatgaa ttttttcagg atcccaaaac
     2341 ctcctccttc aatgtatccc gatgtaaaat catttatgta tttttcaccc ggatcttttt
     2401 caagtgtgag atttatttca agatatttat ctgaattaat agtttttccc gattttccaa
     2461 attttgaatt tacagaagta tagagtttgg gttttgtacc ttcatacttt tgggaaatgt
     2521 aacccgcttt ggtctgcaga tcctgcagaa atcttgatct tacacgtgta tttaatttat
     2581 caaaaaatgt aatttcagat gtgttatatc tgattcccgt ctcatctata attacgtcag
     2641 tctttgatct cctgacaatg atataaattt ttatcatatc ataaagttcg attgacatgc
     2701 cttcgatctt cagaagttca cgtttggatt tgaacttacc aatttcattt ctgtagtcaa
     2761 ttattttacg ggcaatgacg gaactcacaa aaggtatcag atccagttct tcataggtga
     2821 tcatatttaa gtcaagagga tttcgccgga ggttttcgag attgtctaat actgccggtt
     2881 cttcagcttc gggatcgaaa ctttcgagca gcatttcctc acggtaaaaa tttctcaggt
     2941 cggtcgtgtc acctgttttc aaagtatcct gagaaaacag cggtgatgtt gctgcagagt
     3001 acattaagta cataattgct gttaagcaaa gtcttccaaa taattttcgt gtcatggctc
     3061 atcatatttt ccacataaaa ataattaatc ccagccgtga aaaaaaatta aaatttaggc
     3121 atttgtgaaa aaacatgaat tcacctgaga acaaaaacat tgagtttgcc tgagtatcag
     3181 ataggactta cagtctgtct ggaaatgaaa tttttaagcg aaactctgta atgcaatgat
     3241 tttttcaaga gagggtgttt aagaaataaa tcactttttc tttttattga ttttcagaca
     3301 gttcacaaaa tctattccaa gatcgatcca tttagatagt gccttttcag ttttgaatcc
     3361 ggattcattc acatataaaa atccttttaa aggtcttcct gtaaaatcca tttcacgtgc
     3421 tgcctgatca gacaaagctt tttcatattt ctcttcaatc actcttactg tcagatcatc
     3481 tttcattact ccaactgaca ttttaccttt tatcataaaa gcgattccac cgaacatttt
     3541 tttctcttca aattctattc cttttttcat taatgcattg ctgattctct ttgatgtttt
     3601 ttcgttgtat gccatattta aaaattttaa attttaaatt ttaaatttta agataattta
     3661 taaaagtttt ataaatttat aaataaggat tagataattt catatttttc tttctctgaa
     3721 tgtttaaaat tttttgtaaa aaatctcacc ggatatttta gaaatcttca ggtcttcatg
     3781 atttgaattt ttaataaagt atcgagatct atttttgggt tcattcctgc aagtacatct
     3841 tcccaacctg aatcttttcc gtataaagca ctcggaaatc ttttgttaaa cgaattaacc
     3901 tgtgccattg ccattcctgt ttcatgaccg atagatcaac attaaaaagt ttaaatatct
     3961 ctgcgttttt attacttacc ggatctggat tgttcaaata agtttattag ttttaaatta
     4021 aattttgtct gatatttcca gactattaaa ttgctttaat taattcctat ttagtattta
     4081 aaatcttacc gcttgtgaag tgaaatttca tttatataaa tttaaaaaat tttatttaat
     4141 acattaagtt ttgattctgt aaagataaat attataatta tatgaaatta gtaaattaat
     4201 tatattgaaa gaaaaatttt tataactcta attttaatat ttattaatga gactacttat
     4261 tgacatggac ggcgtcattg cagactttga aggagatttt ctgaagagat ggaaagatct
     4321 tcatcctgaa aaaaattatg taccgcttga agaaagaaaa ggattttatg taaaagaaca
     4381 atatgaggaa gatcacagag atcacgttca ggagatctat ctcagccccg gcttctatca
     4441 aaacctccca cctatacccg gtgcacttga agcactccga gaaataaaaa attctttgaa
     4501 taatgtcttt ctctgcacaa gtcccatgct tcctaaatat gagaactgtg tccttgaaaa
     4561 atttcactgg gtatatgaac atctcggcac cgaatgggta aacaatattg tccttacaag
     4621 agataaaacc attgtgagag gcgatgtatt aatagatgac aaacctgata ttaaagggtc
     4681 cgccgagcct gaatgggaac acgtgattta tgatcagcca tataacagaa acataaaaga
     4741 taaaagaaga cttacctggc aaaactggaa agaagttttg gggatttgat tgcctctaag
     4801 tcgctgtgac taaaggattt attatctgac cttcttataa aaataagttg tttttaatca
     4861 aagttttaaa tacataatga aagtattcaa cttgaaattt ttaatactta cttcaattat
     4921 atttttaata caaatatttg atgtaaattc tcagaccatt tccaatcagg tgtttgaaat
     4981 aattgaactg caggataaaa ggtcacttag tataaatgat aaactaaaaa cttatctaca
     5041 ttctctcgat tcgcttatcc gtgaaaaatc aatttacgct cttgctaata ttggtgatag
     5101 taatacagtc agttatctcg attttctttt tgcaggtccg ttcagtgatt atccaaagaa
     5161 gagtgatatc aaagctgcag ctttcatgct cggacagatc aactgtgatg caagtagaaa
     5221 tatgcttagc actattttaa agaacagatc taattatgaa aatgaaatgc ttgaatcggg
     5281 aaaatatgtt atcgaagctg ttggaaagat tgggaatgaa aatagtcttc aggaactcat
     5341 tcagagagag gacctcatct attcttcaga tgaacaaacc cgctctgctg ctgctatggc
     5401 aatcgccggg tttgcacttc gaaggatctc aaatgaaatg tctgttgaat ttttaaaaaa
     5461 gatcgttagc aattcttcag atacaatcac tttaagaaat gcagcatatg cattctggag
     5521 aacaggtgat aaaaatttac tcctaaatgc ccgacaggaa atttatgatc tcgttgaatc
     5581 aaaagatgca cagacgagaa tgtgggcata caatgcactc ggaaaactta aagataattt
     5641 acttcttcta tacactctag aatcattcac ttctgaaatt gactggcgtg taaaagtaaa
     5701 catgctgaac tcactcagaa attataatat tgattcaaca gcagaactct ttccgcagat
     5761 cattaccaca cttggaactg ctatggacga cataaatcca aacgtgtcac tcacagctgt
     5821 aacagttaca ggacagttgt ttaaagatat taaaaactca aagaatgaaa ctgcaactgc
     5881 attatctgaa ttaatgaagg acgaacttat caatgcaatg aatacagaag attcactttc
     5941 ttcagttcaa agaactgaaa ttgcaaatac attatcctta atgtacagag atgaaatcaa
     6001 agatgatctt ttcaaaactt tttatgaaac taatgactat gatctgaaag gcggcgttgt
     6061 cagggcattt ggaaattttg acgattataa aatttatgat gaagtgcgtg acagtgtcag
     6121 cgctgaagtg aaaagatata acgacagaaa taatattaca atgggagata tgatttccgg
     6181 taaagaactt gcgaagatat acagaggctt tgtggaaatg ctgtctgctc agatgtcaaa
     6241 aacgaaaggc gaagacagaa atcttttcag gctgatgttc acagagtttg tcggatcgaa
     6301 agatcctgtt ataacggaaa tttgtctcac ggctctgaag gatacaatat ttagtgaata
     6361 caaagatgaa actgcttcta taattttatt tgattataat gaacttgagt atccgcagga
     6421 cgaagctgtc ataatggttt tcattgacgc tatgaaagat cttaataata aaaatactat
     6481 tgaagtactt gaaaaaaatc tgagctatgg aaattatgat attgcaaaag catctgcaga
     6541 tgcacttgaa aagatcaaat tcaaaagata ccctgttcac tttacgcaga ggtctgatct
     6601 tgataaagct tacttagaat tgatggacgg gaaaaagaat attgtaatca aaaccaataa
     6661 aggaaatatc agaatagaat tatttcccga tgctgctcct cttacagtgc taaatttttt
     6721 aaagctatct gaaaagaatt attttgacgg tactatcttt cacagagtcg ttccgaattt
     6781 tgtgatacag ggcggagatc cgacaggtac aggatacggc ggacccggct attctataag
     6841 aagtgaattc tcaccggtcg attttggtac tggatatctt ggaatggctt ccagcggtaa
     6901 agacacagaa agcagccagt tctttatcac gcattcagcc actccgcatc ttgacggcag
     6961 atatactgtc ttcggaaaag ttacagaagg actggatgtt gttgatatta ttatgattgg
     7021 ggatgtggtt gaggatgtgg tcgtggagta ggattaaatt tttcagtaaa aataaacatt
     7081 gaaataatcg gacaggaaat ttaatttgaa aatagttttg gaaataagaa ttcataaaat
     7141 ctaaactgaa ttctgtgaaa tgttttaaag gattttttaa atatattaaa ggagaaaaat
     7201 agtagaatga acagattaaa aaatttctaa taacaccttt gaagaatatt attttaaaaa
     7261 attttacaat aggattgtct atttacgggt tctgacttat ttaaataatt aattttaaaa
     7321 aacatttgta ggctcaggct ttagcctgag gtgtagttta gaagtgaagt tcaattttaa
     7381 agtaaattta ttgtaggctc aggctttagc ctgagggatg attggtattc gaatttcaat
     7441 aattcagata aaatatatgt aaccgcatgt tttaacgtgc gaaatgaaag gagttgatga
     7501 atttgatcag agaaaaaaag cacagattag agaaagaatt ttatattgga gtaaaaacag
     7561 tttcctttac agcttgtatt aaaaaagaag aaaaagtatt taccaacaat tttatttttt
     7621 caattttcag agataaatta attgaatcaa taaaaaaata tggttgtgag gcattaattt
     7681 atttattcat gccggatcat ctgcatttaa tcctgaaagg taaaaataat gattcaaatg
     7741 ttattaaagc gatagattta tttaaacaaa aaaccggata ttggttttca cagaatatgc
     7801 tttattcaaa atggcaaaag gattactatg atcatattct aagagataat gaagacctga
     7861 gaagccatat ttattacatt ttaaacaatc ctgttagagc aggcatagta agtaactgga
     7921 aagattatga gtttaaaggc tcgacagtat ttgactttga taagtgggat ttgaatcaat
     7981 aaagaaaaac aactagatac tttaaagagg atgtgattga cattatatta aatagatgat
     8041 tactttgtat ttattagttt cacgcagtct aaagtctgcg ctaacgaatc gtttctttta
     8101 aaaataaaat ctagtattac tattgtgttc cttgtgtttc attcccgcag tctaaagact
     8161 gcggctacaa atcgttcctt taataaaata tatttaatat tactgttgcg ataccatgtg
     8221 tatcattccc gcagtctaaa gcctgcgcct acaacgannn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnggt agaaggacaa cttgtcgtgc agcatgcaca cagtatgcat
     8401 tttgctgatt cgaaaagttt ctccgcgtcc tcgtttgact gaagtctctc gatgtcaggc
     8461 ggtggcggtg aattatttat gagataaggt tttataactt catatttatc gaagaagtct
     8521 gtcatttcaa ctactaaatc cttaatgatc ggaagtgcag gcagcggatc tatcactatc
     8581 ggcttttttg tgttaatgta ttttaacagc agagcatatg caagcttgtt cttaccattg
     8641 accttcatgc cgtcgcttcc gcagataccg tgagcacagg atcttctgaa tgtaagagag
     8701 ctgtcataat tccattttat ttcatgcaac acatcaagca gccgccagtc ttaactgaca
     8761 ttctttaagt tgaaatcctg atagtacggc ccttcatctt tctccggatt gaatcttaaa
     8821 atttttacct gtatgtccat aatgaatttt tgaaaaatga aattttattt cagtaaaaaa
     8881 ttaattctta atgaatcttt aaatccgtcc ctgtcaggac atttgtaaaa ttcatctatt
     8941 aatttatatc cttcatctac tttgccaagc agcatataat cttccgtagc tgctgccagt
     9001 attgccttta ctgcacctgc atcagtatta aatgtttcag tccctgcctc ttcacattta
     9061 aaatttttat attcatcaag ttctttttta agctcagtca gattatcatt aatctgcttt
     9121 ggaaattttg cagttacatt cacaaacttt ttattctcat atccataaat tagcaacgga
     9181 aatttcgact gtgcataatt tgtaaacata tatgcataca catcattaaa tccaactatc
     9241 tctgtctttc cgtcagcgtt tagatcttca gctttgaaaa aagaattccc ccagataatg
     9301 gtatcagtaa aattaaactt ccccttgctg atctttcccg tgaataagct cgtacagcag
     9361 tgagcacctc cgctgtacat tgcaatcaga tattcttttt tgccgtcgtt atccagatcc
     9421 atccttgcga tctcaggtat cagatcataa taaatctctt caaaaatttt ctcatcctct
     9481 ctgtaaatgc tgagagtagt tgtatattct tctgaattaa atttcgtctt aaaaatattc
     9541 tttccgaatt ttaaattcag atcttttacc tgtgcaaaat ttctttcatt aaaaaaaagt
     9601 acaaatatta cgcaaagcaa aattaaactg aagcagtttt taaatttaat gtttatcaaa
     9661 tcaatacttt cttttccatt ggctgaaatc ttgtcactat taccggtttg aatttcagat
     9721 caggtacttc ttgtcctctg ctaaaagcaa aggtatgctt catccaattc tgatcatctc
     9781 tctctggaaa atcctctctc gtatgagcgc cgcggctttc ctgtcttgca tatgcgccgt
     9841 acacagttgc ttccgcattc agcagcaaat tatcaagctc aagtgcttcg actaaatctg
     9901 tattaaatac tttactctta tcctgaactg caatattttt atatctctct ctcagctctt
     9961 taagtttctc aatcattttt ctcaaacctt cttcagtcct gaatacaccg cagtcttcca
    10021 tcatggtatt ctgtaattca gttctgatgc tgacgatctt ttctttcgga gcaaggtcca
    10081 tcacagttgg agcatatctt gccatgaatc tttcgccgtc cttatttttt aaaattccgc
    10141 cttcgcctct tgcagcttca cttacaagaa ttccaagctt ccataaaccg gtcggatgga
    10201 actggtaaaa ctccatgtcc tctaaaggaa gaccggctct gtaagcaagt gtcattccgt
    10261 caccagtacc gacatgtgca ttggaagtga tcctgcatat tctaccgttt ccgcctgtcg
    10321 caaatagtac tgcctttgcc tgaaaaatat gtatctcact tgtcgcaata tcaaaagcca
    10381 ctacaccttt acatacacca tcttttgtca gcagctcagt tacaaaatat tctgaaaaga
    10441 aagttacctg atttttaatt gtattatcaa ataaagtctg aagcataaca tgacctgttc
    10501 tgtctgcaga ataacacgct ctctttatag gaagtctttt tccataagga tcatttttat
    10561 cagcaggttt tgtatgtcca ccgaattgtc tctgagcgat cttgccgtct tcatttctgc
    10621 tgaaaggcat tcccatatgt tcatgctcaa tgatagcgcg gatcgcatct ttacacaata
    10681 cttctataca gtcctgatca ccgagatagt cacttccttt tacagtatcg aatgcatgat
    10741 tttcccagct gtcttcttcc atatttgcaa gtgctgcaca gacaccgccc tgagccgttc
    10801 cggtatgaga tcgtgatgga aatacttttg atattacagc acatgaatat tcttttggaa
    10861 tttcaacggc agccctgaga cctgcaccga ctgcaccgac tataactaca tcaaactttt
    10921 ttatcatcta tatatcaatc acaaaatttt gttacttata aaaatattta tgattttact
    10981 catcaagttt ttttatttct ttcagaagtt ttgttttttt cttgacagca tcttcataat
    11041 atccggcgtt gcttttgaga tccataaaat aaagaatcgc ctctaacttt ttttcttcct
    11101 tcactgctat cattccaagg taatatagca tgtcagtatc ataagttgta taaccggttt
    11161 gttcaagctc tttgttatac cctttttcaa gataatattt tgctttgtta atatccttga
    11221 tctgctcata gtatattttt cccaaacgtg tatatgcctg aatgtaggtc ggatctttaa
    11281 caagaagatt cttatattca gcaacagcat ttttataatc ttttttctct tcataaatag
    11341 ctgcgatctg aaaattcaca tagtacagtc tgctgtcttc atcgagcatt ttataataaa
    11401 actttaaagc tttttcataa ttaccggtac ttgaatatgc agcagccagt ttggatgata
    11461 tcagattcag caaactgtct gaatcaaatc tttttaatat agtttcgtaa agcacttcag
    11521 ttttttcagg gttgtctgta tttacataaa ggtccagcag ctcttcatac acaaagaaag
    11581 aatccggctg caaagtcaga caattattaa aaagttcttc aaccttaaca aacctgactt
    11641 tatttataga gtcctgtctt aatgtaaatt catctttgct gtccagcgaa tcttttaatt
    11701 ttgtataata ccagtccgca gcaagttccc atttctgatt aactgcattc ttgaatgttg
    11761 tattaaaaat attctcttcc ttttttataa gcatatcatc tgtaatttca gattcattca
    11821 gaagagcaaa atccatgaat ttcttaaatg aattaattgc tattgcaaaa ttcagattac
    11881 cgtatcctaa gtaagtgtaa gttgtaatac cgataacttc accttttctg ttaaataaag
    11941 ctccgccgct gttaccgggt gagatagctg ctgtgatctg tattactttt tcaaataatt
    12001 tttcattgcc tgtgtaagaa tatggatcac caaaatttac tttttcattt tctcgaaccg
    12061 ctgctactat acctgatgag atcgtatatt caaatccgag aggacttcct attgcaaata
    12121 cttcctgccc tgcttttaaa tcatctgaat ttccaagctt tacaggctta aatgtaatgc
    12181 cgttggaatt tactatcctt atcaatgcaa aatctttttc tgcatcagac attacgactt
    12241 ctgcataaaa gaaatttccg ttgctggtct tgatgatcaa actgtctata ccttctatga
    12301 catggttgtt tgtacctata agaccctctt cattaagtat aaatccactg ccattaagta
    12361 aagaagtatc aatgtatttg tcctctccat aggaataata atctgtctgc gatttccata
    12421 tcgagatcag tgactcttta ttattatcta tgatctgctc agcattaaaa tcatcatcag
    12481 aaagataatt atcagggctg acagttttat cctgcgcaga gacattttca caggaaataa
    12541 aaataataaa tactgatgta agaagtttta acagatttaa aaaataacgt aaatcaaaaa
    12601 atttattttc ctttattata tttaagatca tttgcctttc cattcagctt ttcttttttc
    12661 tacaaatgct ttcattcctt caaacttatc ttctgttgca aacaacagat aaaaattctt
    12721 tctttcaaat tctaatccgg cttctatcgt cgaatcaaat gatttcaata tgcattcttt
    12781 cgccagctga actgcaatag ccggctttga ggaaatttct tttgccagct caagagcttc
    12841 atccaaataa atttcatttt ccactacttt agtcacaagt cctgcatcat acatttcatc
    12901 tgctgtatac atttttcccg ttagtatcat ctccatcgca cgcgcttttc ctatagcttt
    12961 tgtaagtctc tgcgtaccac ctgctcccgg tattaccgca agattgatct ccggctgtcc
    13021 gaactttgcg ctcttcgacg caactatcat atcacagctc atcgcaagtt cacatccgcc
    13081 gcccagagca aacccgctga cagcagctat taccggcttc ttgatctttc ttatcctgtc
    13141 ccatcttgca aactgatctc tgatcagcat ttctatcgca gtcgcttctg ccatctcttt
    13201 aatatctgca cccgctgcaa atgctttcgc atttcctgtc agtaccatgc aacccgtttc
    13261 actgtctttg tcaaaagctt caagtgtttc cactacttct ttcatcagat cattgttcaa
    13321 agcattgagt acatcaggtc tgttcaattg tatcagaccg actttcagtc ctttgggatt
    13381 ttcaatttcc gatacgagta tattattaaa tttacctaaa aggtgggatt tatcgctcat
    13441 ttatataaat atgtgattaa attacttaag attgaaattt ttgtaaatta ctgacattga
    13501 gctataatta caatgttaaa taaattggaa tttgtataaa tctttttaaa ataagaagaa
    13561 aatagaataa cgataagctt tcgctaaatc aaaaattcag aaattgaatt ggatcagtta
    13621 gtaattcctc caaaagtcag aaagcatttt ggtataaaaa gcggaacagt aatacatttt
    13681 atttatagta atggcgaaat aaaaattgta caggttacaa aagaaaccat aagcactaac
    13741 tttggaatat tcggaacaaa aggtaaagta ttaaaagcgc tcaagaagga aaaaaaaatc
    13801 tgaacgtgaa ttatgaaaaa atatgtactc gatagttttg caattatagc ttatctggaa
    13861 ggagtggaat caggcaaaaa gataatctca attttagaat aagctttaga aaataaggaa
    13921 aataaggttg atttgtttat gagtgtaatc aattggggag aagtatatta tatcattgcg
    13981 ggaaggagga atagaaagag cagaaatgta taaatcaatt atatcaaatt atccgattga
    14041 gttaataaat tctgataaag aattaagatt gaatgctgca aatatcaaat caaaagtaaa
    14101 actcagttat gcagaggcat ttgctgccgg tctggctaaa atgctggatg caacactttt
    14161 gacaggtgat aaagaatttg aacccttatc tgataaaata aatgttgaat ggctttgaga
    14221 aatttatatt gatcataaaa catagatcat tgaacattaa aaattgaaca ctgaacatta
    14281 aaaattatct gttaacaagc atttcaatat ccgcttccgt ttccctcaga tcaataccgt
    14341 gttctaatac cgatttcaga tttgtaagaa agaaaaccca acacacacaa caatttataa
    14401 atccaaactc attttcagta gcagaatcct gatagcattg agtaagtaca agcttagttc
    14461 tgtctcctga ttcagacaat ttaatattca caaaaaacga agatccgaat gtaaatccaa
    14521 actcattctt tccgtcagat tcagtaatga ttccttttag ttccagatct ttcttcagcc
    14581 atttccataa aaatgaatta cctttttgtg caggttcatc tccaagccgg atacttttgt
    14641 cattataaaa ataatcagca ctgccgataa accattttga caatcccgac cctgttgaaa
    14701 tatattcata tactttttca ggagatgcct tcaataatat tccgtgtgaa aaactttctt
    14761 tgttgaaatt ttcttcagtc attatattca gttaacatat tttatttttt attataggaa
    14821 tttcatctat cgaataataa accggaagtc ctttagattt tatgaaatct ctttctctgt
    14881 cggctccagg actgctgccg atcataagta tagcttcaca cctgtcgatc acagccattg
    14941 agatttccat tatagcttca tacttatttt caatattcga tttttctatg actggcaatg
    15001 cagcatttac accgatcaga ggaatgtgcc caagttcaag tattttcgct gccgcttcat
    15061 tcattgcatc aagatttgct tttcgagcag cttcagtatc ggcagaaaaa ggacctgcaa
    15121 ctccaatgat cattataaat tatattataa attgataaaa taaacgaata taaaattcgg
    15181 aaagatagta tcttcgctga gtcacttact tttcacttac ttttcggact atgtggaaac
    15241 tttttgtacc cgcagagtca cgtacactac ggattctgaa ttaaatgtta aaatattaga
    15301 atagaataac agaaatttaa ctgtttattg tgtcgtgaga agctgtacaa ttacaatttc
    15361 atttccataa agatcaaata cgctgacaga tttaaattct tcagtaattt ccggttcact
    15421 tttatattca attccattat ctgatatttt ttcattcagc acatcgaaat catcagtata
    15481 taaaaccaat acaggtgctc cacctgtctg tctgccgact aaactttgct gttcagttgt
    15541 atttgctttt aaaaaccata ctcctgcacg ggtctcactg tcaaaagaaa tgtgaagaaa
    15601 tctttctccg tcgtcatttg taatgtcaaa aaattttttg catccgaaat tatctttgta
    15661 aaattcaaat gcttcatcgt aatcttctac aagaattact gccctgccga gatctacttt
    15721 catgataatt tatttatttt aaaaatcagt tttgaatttc aaactccata agaaattcat
    15781 cgtccttgta aaacataagt ttttcttctt caagtttaaa attatttatc agatggatat
    15841 ttttatgaaa tgaagaaaat acttttacat tttcgcacat catttttgta gtaatcattg
    15901 gtaaaaattc gatcttcccc gaatcaattt taattttacc gtttatacta ttgcatccgt
    15961 cccgtccgtt tattctgttt tcatccagat caaacttaat ataaacttta ctaaagtcag
    16021 attctctgtc aaagttttct tcagttttca ttcccttcat gatatatgat cttttatcca
    16081 atttcactct gtcagtatct gatattacca ttgtaggttc ggcctttaaa atattaatca
    16141 gattataata aaatgaagca tcatctgcag cctgcgttgc aacaaacaat ttttcaactt
    16201 caataatgta ttcatttccc ggtataaatt caaaaccttt gatgtcgtta aagtgaaatt
    16261 tccattcctc agattcagaa tttttgatta ggaaacattc ctgcggagct acacctatac
    16321 agcttacttt atgatttgca atatagagtg tatcatattt gctttgagaa attgcagtgc
    16381 agataaacaa atttagaata aatacagtga gaattatttt cattgatcca tatattacag
    16441 ataagtgttt ttaccaattt aaacattatc agaattttat acaattgttt tcaatatttt
    16501 gaatatcatg atccttaatc ttagaaaata tttttagaat taaacagttt aacattccaa
    16561 ttttttttta agattattat attgtctata tgaaaaaaaa gaacgttaaa gaaaccaaca
    16621 gcatcaacat tccaacaata atgcggattg catatatcga cagacagatc tcgatgaata
    16681 attatccgtc agcaataaaa ctttcaaaag aatatgaagt cagttcgaag actattatcc
    16741 gtacaattga attcatgaaa agtttttata atgctcccat cgaatatgac aaaagtataa
    16801 agggatattt ttattcagac aaaaatttca aactggttaa attcgatctg aatgatgaag
    16861 acctgcgctc acttgctttg ataagcaatg cctttaaaat ttacggcggt aaaccttttt
    16921 caagtgatct gaattctttt tttaataaaa tttactatat ctataatgac aagtacagca
    16981 tacagctcaa agacatagaa aatattatca gcttcaggat caggaattca aggtctgtag
    17041 atactaaatt ttttgagata ataaaaactg cgatcataaa ttcagaaact ttaaagtgcc
    17101 ggtattattc agggtattca ggacagacta ctgacagaga actcgatcca tatcatattg
    17161 tcaacgataa aggtgaatgg tatttaattg cattttgtca cagagataat aaaataaaac
    17221 tattttccat cagcagattt aaggaagtac atccgtcggg aaaacaattc tcaccggttg
    17281 atgattttaa tgttgagaaa ttttttgagg acagctttgg agtttttaat gatgacaaaa
    17341 aaattaaagt aaggatgaga tttgaaaaaa ggtctgcagg ttatataaag gaaaaaacat
    17401 ggcacagatc tcagatcata aaagaaaata aaaaggacgg cagcgttata ttggagatga
    17461 ctgtaaacag ttcagacgaa ataaagcact gggtactaag ctggggtaga tcctgcactt
    17521 tgctttctcc aaaaactctc gtcgatgaaa tcaaaagtga tcttaaatcc actctcaaaa
    17581 attattcctg ataaaaattc tttatcactt tagattatat aaaaatttaa taattacgtt
    17641 ttacttttta caaaggacat tgtttgtcct ttaaacattt taacttgtat aacaattata
    17701 acaatagagg aagaaccaat atgaaatccg gtacttcaga tttcgacctg cctcactttt
    17761 tttagtattc atttcgcaaa ttcggagatt gcttcgcctg atgatcacag gctcgtaaag
    17821 acattttttt ttcgaattgc ttccacgaat gaattcgggt tcgcaaaaac ataaaaaaaa
    17881 cccgcaaaga agtcaatcat ctctgcggga tagaaatact taaaaattat ttatttgaaa
    17941 tttttaattt gtaatttgaa attgaatcaa ctgcatccgc tagtttcacc acaggtaata
    18001 cacttcagac acgtaccgtt tcttaccatc gtcacgctgt ggcattgctg acaaatgtca
    18061 cctgtgtaac ctttcagctt tgcctctctt attttttcca tagatcgggt ctgagactgc
    18121 tcctgagttt ttgcaagttt aattttattt gatgcagcag ctgttgattt atcaggctta
    18181 ttaaaatcta tgcccggttc atcaattatc ctttcactgt aaacttcctc ttcatcaaag
    18241 tcagtatcat tatcaagttt actcaaagca tcaataggtg atctctgata ctcggtatta
    18301 ttttcaatat gtgaaagatc atttctgcta agataagtta cagccagctc tctgaatata
    18361 taatcaatta ttgaagtact cattttaata ttcggattac caacgactat tccgttcggc
    18421 tcaaatcttg tatatacaaa cgcatctaca aattcatcca acggtacgcc gtgctgaagt
    18481 ccgagcgata ttgcaattgc aaaacagttc atcagacttc tgaatgctgc accttcttta
    18541 tgcatgtcga tgaagatctc ccctagctgt ccgttttcat attcaccggt tctcagatag
    18601 acagagtgtg ttccgatctt tgctttttgt gtataacctt ttcgtctgaa cggaagcttt
    18661 cttctctttg caatatatct gtgaattatc ctctctgcaa tttttacaat gtcatgagat
    18721 ctggatgctt cagtatttat atcaactgaa ttattgtaat caataatatc gtcatctttt
    18781 atactgttca gcggctgaga taatttcgat ccgtctctgt aaagtgcatt ggctttaatt
    18841 ccaagtctcc atgaattcag atatgcattt ttcatatctt caatagtagc atgatcagga
    18901 agattgatcg tctttgaaat agcacctgaa ataaacggct gagctgctgc cattattttt
    18961 atatgtgcat cagctaaaat atatctcgtt ccttttttac cgcatttatt tgcgcagtca
    19021 aaaattgcat actcttcttt cttaagatat ggcgcacctt ctacagtcat agtaccgcag
    19081 acaaaatcat tagcttcttc gatctgctct tttgtaaagc cgagttcctt aagtatattg
    19141 aatgacgggt catctgtttg atcttctgaa aagccaagtg catttttaca aaagcctgag
    19201 ccgagcgtcc atttattaaa tgcaaaatta atatcaaaaa ctgacggaag agatttctca
    19261 actttagaaa tgatctcttt tgtaaatcct ttttcagata atgactgtgc atttatgtgc
    19321 ggacatccga tcagcgagcc gtgacctttt gtatatttaa ttatttcgtc aatttctttt
    19381 tcgttgtatc cgagatttct cagagcgggc ggaacagact gatttataat tttaaaatat
    19441 ccgccgccgg caagtttctt aaattttacc aaagcaaaat ccggctctac acctgtagtg
    19501 tcacagtcca ttagaagtcc gatagttcct gtcggagcta ttaccgttac ctgtgcattt
    19561 ctgaatccgt gtttttcgcc aagctcgaga gctttgtctg catcttcgat cgcagctttt
    19621 attaaaggct cgggacaatg atttttattt attcccatcg gagtgatcga cagatcttcg
    19681 tattcacttg cgcctacatt gtatacagct cttctgtgat ttcggatcac gcgaagcatg
    19741 ttctgttcat tctctttata tctctgaaac ggtccaagct cttttgccat ttcagctgaa
    19801 gtggaataag atgtcatgtg aagaattgca gtaagtgcgc cggttataga aagtgcttca
    19861 ggagaatcat aaggaattcc ctttaccatc agcaatgcgc cgaggtttgc atagccaagt
    19921 ccaagtgtcc tgtattcata agaaagtttt gcaatatttt tactcggaaa ctgagccatg
    19981 agaacgctga tctctagtac aattgtccat agtcttgatg catgtctgta tctgtcaatt
    20041 tcaaaacctg tcgtttcatt attataaaat ttcatcagat tcagagatgc aagattacat
    20101 gctgtatcat cgaggaacat atattccgaa cacggatttg atgcaatgat ttttccgcct
    20161 tcgggacatg tgtgccagtc gttgataata tcgtgatact gaattccggg atctgcacat
    20221 gaccaggcag cataagctat ctgttcccaa agatcccgtg acttataact tttatatggc
    20281 tttggctcgc ggttttctgc ggaagctttt tttaattccg tcctccagta tgtattccag
    20341 tcaccgtctg ctacaactga ttccatgaat gcatttgtaa ctctcacgct gttgtttgaa
    20401 ttttgtccgg atacagtaag gtatgcttca gaattccagt ctgtattata ttccggaatt
    20461 tccatttctt tatatccaag ttttccaagc tgaataacac gctctatata attttcgggg
    20521 atatgaattt ttcttgcatc gtgaattgcc tgcgctaatt gtttatttgt gtttttgtca
    20581 aagccgtcat tttcaggatg aacactgtga catgctttca tgatcttatt aaggtaaaca
    20641 tttagaagtt ttgaacctgt tacaagagct gcgactttct gctcttccat tcctttccag
    20701 tttatatact cttctatatc aggatgatcc atatcgagag ttaccatttt agcagcgcgt
    20761 cttgtagtac cgccggattt aattgcacct gcgcttcggt ctccaatttt tagaaatgac
    20821 atcagccctg aagatctgcc gccaccgctg agtggttcgt tcactcctct tacatttgaa
    20881 aaatttgaac ccgtacctga accatattta aataatctcg cttcgcgtac ccacagatcc
    20941 attatgcctc cgtcattaac gagatcatca gaaagtgatt gtataaaaca agcatgcggc
    21001 tgtggatgtg tataagcgtc ttttgaagta gtaagttttc cggagatcgg atctacataa
    21061 aaatgtccct gcgatggtcc ggtaattccg taagcccagt taagtcctgt gttaaaccac
    21121 tgcggagaat tcggagctgc atactgcata gccagcatat aacaaagctc ttcgaaaaaa
    21181 ttcgcagcat cttcttcatg atcaaaataa ttgtgcttcc atccccagta tgtccagcaa
    21241 cctgcaagcc tgttgaatac ctgtctcgaa tcattttccg aagtatatct ttcttcttcg
    21301 atcacgtctt taagtttttc atgatcaggt tcagatctct gaagccagcc cggaactccc
    21361 tcctcttcca cttttttcat atagacaggg actcctgctt ttctgaaata tttctgagca
    21421 agaatatctg tagctacctg agtccagtgg tcaggtactt tgatatcgtt catttcgaat
    21481 actatagaac catcagggtt acgtattaca gaagatcggg aatcaaattc aatttgatca
    21541 taagctgaaa tgcctttgtt tactaggagt cgggatattt tcatttacat atatatataa
    21601 gaattttgat aaataatttt taaaatgaat tagttaatca gacattttta ttaattcagt
    21661 tttaaaatat tacaagtatt agttggaggt attttgtaag attttatttg taaaattatg
    21721 agaatccttt tttaaatgca atcgctttta taaaatatac ctattgattt taaattattt
    21781 ttttaatgaa aaatattttg atgtaatttt tcaatatttt gtgttttaaa aagtttgaaa
    21841 tcgttaaatg atttttaaat taatactatc acacttttaa attaattaaa ttatttaaaa
    21901 atttttttaa tatttatttt tttgttatga taaaaatttt cacagttgat gcttttaccg
    21961 acagaccatt ctctggaaac ccggcggcag tatgccttcc gaacgttgag cttagtgagg
    22021 aagtcatgca aaagatcgct ttcgaaatga atctttccga aaccgccttt gtcacaaaac
    22081 ttgatgacgg attcagtctc agatggttta ctccgaaagc tgaagttgaa ttgtgcggtc
    22141 acgctactct tgccagcgca catatacttt ggcaggaaaa aatgatttcc gaaaatgaag
    22201 aagcagtttt ttattcaaag tataaaggaa ttctcaaagc aaaaaagtca ggcagcgaga
    22261 tcactctgaa ctttcctgta aacaaaccat taccgtcaga tagaaatgaa attctcgaaa
    22321 aagcattagg tgtaaattct gtttcactga atactatttt tacgacagaa catcattata
    22381 ttgtcgaatt aaattccgaa gagaatttaa aaaatgtaaa acctgatttc agtctgcttg
    22441 aaactttacc aaaatacgga gtaattataa cttcaaaatc cgatgatcct gaatttgatt
    22501 tcgtatcaag attctttgct ccatcaaaag gagtaaagga agatccggtc acaggttctg
    22561 cacattgtgt acttgcaccg ttttggtcag aaaagctcgg gaagaaaaaa atgaatgctt
    22621 ttcaggcttc cgaaagaggc ggggtattaa gtgttgaatt aaaagacgaa agagtattaa
    22681 tttctggaaa tgctgttact gttacagcag gagaattatt tctccataca gacagcttct
    22741 cttaatacat tacaatgcct caaagtatca aagactcaaa gttaaaaagc ttttaattaa
    22801 acgtcttaga aacttagaga cttagagaaa aactaaattt ttaaaatata aatatgctgc
    22861 aaagagatta cttaatgaaa atggtagaga tgcttacggc tgttatggca aaggtgatct
    22921 ttaataaaga gcagaagaag tatgatgaag ctcttactga aatagaaaat gccggtaaga
    22981 atattatcgg tctggatatt ggaatggtaa agatactgaa gatagatgac ttcacaggac
    23041 ttatgaaaac gtccgatgtc tttgccggca gatgtctgat ctctgctgag cttcttttag
    23101 aatatgctaa gatacttgaa gaaaaaggaa ctgacacaga tcttaaaaat gatttttaca
    23161 ataaatcttt tcgcctttat acagaagctt tactgacaaa ggaaattctt gagcccactg
    23221 aatatattga aaaagccgaa agcattttac ctgtactgag taactcagga tattttaata
    23281 ttcagtatga ttactattct aataatctga ccttgtttga gttttataat ttatcaggaa
    23341 attattcaaa ggcagaagat attctgttta atattcttga atccgggtct gaactgtctt
    23401 cggaagatga tgaagaaaat aaaagaaaat taagaaagag agctgtagct ttttataatg
    23461 atctgcttag catggatgaa gaaaagcttg caagcggaaa cttttcaaaa gacgaagctg
    23521 aagatggtct tcaggaaatt atgaaaatga tgaaattaaa ttaaaaatat tatataaatt
    23581 aaataatcaa aacctaaaaa atgaaaaaaa tttttttaac actaattgta ttttttgctt
    23641 tctgcggaaa ttcattttca caaagtgata caaatactgc attaagcgga ttgaaatatt
    23701 taatcctaga ggagggagac ggaccggatg cagaatcagg caaagctgtt gaagttcatt
    23761 ataccggcta tcttctcgat ggaaaaaaat tcgattcatc actcgacaga aatgaaccaa
    23821 taatgtttgt actcggcgca ggtcaggtga tcaagggctg ggacgaaggt atttcactaa
    23881 tgaatgtcgg agataaaatg cgtcttataa ttccatcaga acttgcatat ggaaataaag
    23941 gtgcaggcga tatcattcct ccggatgcga ctcttgtatt tgacgttgag cttgtaagtg
    24001 tcggcgatcc gaaaacttct attgctgaag tattattatc tactatcgta gatgtgaatg
    24061 tacaagctgc tataagtcag tacaaagaat taaaagctaa tgaaccggat atttataatt
    24121 ttaaagaaag cgaacttaat aaactcggtt atcagctttt tcagatcggg aaaattccgg
    24181 atgcacttga aattttaaaa ctgaatgttg aaatgtttcc aaattcatca aatgtatatg
    24241 acagtcttgg tgaggcttat aaggtttcgg gaaacaatga acttgcaatt gtaaattaca
    24301 ataaatctct tgaactgaat cctgagaatg aaaacgcgaa gaatatgctt aaagaacttg
    24361 aggggaaata gttattcatt taaaaaagtg taattaataa tttttgaaga aaacttttga
    24421 atcgcctcaa agtctctaag attcaaagtt aacaaaaata atgaactatc ctgcagcgtt
    24481 gtatcttatt ttatgcttaa aacaaatctt tacgtcacaa gtggtgggga attaaattca
    24541 aatagattaa aattattttc ctttgagcct tcgagacttt gaggcaagct atatattttt
    24601 gatatacaaa tgaattttgt gcaaaaaaaa agcccgtgaa ttaattcacg ggctttttaa
    24661 ttcttaagtt aaaattacat aactttagaa acgttggctg cctgaggacc ttttgctccg
    24721 tcttctactt caaattcaac ttcatcacct tcgtcaagtt ttttataacc ttcagagttg
    24781 atagctttga aatgaacgaa aacgtcttgt ccgtctgctc tggttatgaa gccataacct
    24841 ttagtagcgt tgaaccattt aacagttcct ttttccattg ctgatttctt ttttgtgtta
    24901 gtgaaaatgt actgctgcaa cttttggctc ttaaaacttt ttaaccgttt ggtactttat
    24961 tacctctggg ttaattgctg cgaatctcac tccaaaaaag gatttaatct tacatcagca
    25021 aaatattaaa agcttgttac tactttctta ttttggtaga acaattccgg tctcccggaa
    25081 caaaaacgct gtactgtttt tttacggtta caatttagct atatcaatct gtaaatacaa
    25141 cttatataac catattggta aattaatttt taatccttac ttttaatata tcaaaggttt
    25201 attttatgaa aattttattt ttttggataa agatataata tacaaaggca ttcctgtttc
    25261 acccggtata tctgtcgggg aagtttattt atatgttcgc aataattttg aagtcaattc
    25321 acgggacctt attcctgaag agatagaaaa agaattagat gattttcaaa ggtcagtaga
    25381 gatatctgtc aaagaacttt ccaagatcag aaattattct atcgaaaaaa taggtgaagt
    25441 aaactcactt atctttgatg cccaattgga atttcttcag gataaatttt ttctaagcca
    25501 ggtcatacac agaattcaaa ctgaaaaaag aacggcaggt tatatattta atgatgagat
    25561 ctccaagctc gagtttgcac ttatggattc gaaggataca tatttaaagg aaagatttga
    25621 agatataaaa gacattaaga acagagtact gcgaaatctg aaaagggaaa aacttgtatc
    25681 aaagattgaa gaaaatttaa taatagtatc acatgagctt acacctgctg atacaattct
    25741 tttcagtaaa agaaaagttc tcgggtatgc ggtagataag ggaggcaata cttcccacgc
    25801 agctattatt tcgagagcac tgagaattcc tgctgtagtg gggatgaaag atattacaaa
    25861 atctctgaga agcggcgaca atattatcat agacggatct gccggcataa taataaaaaa
    25921 tccttcagaa gatacactca aagaatacga tgtaaagata ataaaactca aagaacatga
    25981 agagaaactt aaagagataa ttgatcttcc atgtgttact cttgacggaa aaaaaattga
    26041 actcagcgca aacattgatt ttgatgaaga gatagatttt atagtaaata caggacattg
    26101 cggaatcgga ctatacagga ctgagcattt atttcttgaa gaaggcggat tcccatctga
    26161 agaaaaacag ctcgaagaat attcacacat ttcaaatgta gtttatccca aggcagttac
    26221 tatcaggact tatgatatcg gcggtgataa acttcttccg cgttctgaaa aagaagataa
    26281 tccgtttctt ggctggagag ggatcaggat atgtctggac agggaagata ttttcaaaga
    26341 acagctaaga gcattataca gatcttcagt aaataaaaat atcaggatac ttattcccat
    26401 gatctcttct ctggaagagg taagaaaaac caagctgata ataaaagaag ttatagaaga
    26461 atgtgaaaaa aatgatattc caattgataa gaatctgaag attggaatta tgatcgaggt
    26521 accatcagca agtattatgg cagaagaact ggcaaaagaa gttgattttt ttagtatcgg
    26581 aacaaatgat cttatacaat atttactggc agttgacaga ggcaatgaac ttatttccag
    26641 tctgtataag gagtttcatc ccgccgtgat acgcaccata aaaattatag cagacgctgc
    26701 tcataaaaat aaaattaaga tcagcgtatg cggtgaaatg gcttcaaacc ctttagcagt
    26761 cccagtgttt attggacttg gtgtagatga gctcagcgtt tccccttcga aatttcctga
    26821 aataaaacag atcataagat cgttgcattc caaaaaagca tacagacttt gtaaagaaat
    26881 tctaaaactt tcttctgaaa tggagataaa agagaaagtt gaaaatttca atgaagcaat
    26941 tacgaatgaa atcaaagtca tctgaatttc cctgcatttc aactcatttt tcttttcaaa
    27001 aaacttcttc atttgcattt aaatttaaaa atattttaca gaattttggt acttaattat
    27061 aattagaaat gccggaatat cttattgaac ttattataac actttccaag ataagtgttg
    27121 tacttgctgt gttactgacc ggtgtcgcat actcggtcta tgctgaaaga agagtgtcag
    27181 catttataca aaaccgatac ggaccaaaca gagtaggacc gcagggactt cttcagccgc
    27241 tagtggatgt aattaaatta ttgctgaaag aggatatcgt acctgaaaaa gcgaataaat
    27301 ttattcactc tctcgcacct gtcatctcaa ttatagtatc actttctaca tttgctgtaa
    27361 ttccttttgg agatactatt actattggag acagagtgat caaacttcag attgcagatg
    27421 taaatatcgg tgtgctttat tttttagcac ttacttcact cggtgtctat ggtgttactt
    27481 taagcggatg gtcttctaac agtaaatatt ctttgctcgg tggattgagg tctgcggcac
    27541 agatgatcag ttacgaacta gcgatggggc tttctgtaat tgccgtaatc cttatgaacg
    27601 gttcgctgca gcttgataca attgtatcaa atcaggcggg ctggaaatgg aatattattt
    27661 tacagccggt tggatttata atctttctga cttcggcatt tgctgagact aacagaactc
    27721 cgtttgatct gcccgaagca gagcaggaac ttgtcggcgg atatcataca gaatattcaa
    27781 gtatgaaatt cgcattattt ttcctcgcag aatatgctaa tgtaattata tcttcagcaa
    27841 tgatagcgac tatttatcta gggggctggc aattccctta ccttcaggat tttggattac
    27901 cgccaatggc tgtaagcatt ctgcagattc ttacatttgt agttaaagta gtagctctag
    27961 tatttttctt tatctgggtc agatggacca ttccgagatt cagatatgat cagcttatga
    28021 atcttggatg gaaggtaatg cttcctcttg gaattgctaa tcttgtaatc actgcaattg
    28081 taatattatt aatgaaataa aaatttttta agtaaataaa aaaaacgtaa tgaaaaaaat
    28141 tattttaaca atattgttgg ttttcgtatt tgcatttaat ttaagtgcac agacttataa
    28201 taatttcaca cttgaggatc tggacaataa cgaaattaca cttgatgatc tgattaaaaa
    28261 aggtcctgta ttaatctcat tctgggcctt atggtgcgga ccatgcaagg aagaaatgaa
    28321 aaaaatgcag ccaatctatg aaaaatataa agatcagggt tttacttacc ttgcaataaa
    28381 tcaggacaat cagaaatccg tttcaaaagt taaatctttt atcaacgcaa ataaatacac
    28441 ttttcctgta gtgctcgata ctgaaaatga tgtctttgaa gcatataacg gaattggaat
    28501 gccttactcg attcttatag atatgaataa aaacattgcc gcaaaacatt tgggttatgt
    28561 cacaggcgat gataaaatta ttgaacagga aataaaagaa gttctgatgg ctggcagtaa
    28621 ttataaaggt gataaataaa ctttttaatt tttttatatt ttaacgggga tagttaattg
    28681 atcaggaaag tatttataat ttctttattt gtctgttgtg caaatatttc ttttgggcaa
    28741 ataaacctcg atgtggatgc atctgtcagc aaccttctca gatacggaaa cggttatgaa
    28801 tattcaggaa atgtaagaaa tgccaaagaa tattttgaaa atctaacaga tgcaagactt
    28861 aaggtaaacg gaattgtatt cggattaaga tataatgtca gtaatccgat cgagtatgga
    28921 caaaattttg tcggaataga taaaagatat attcaatacg agcatgatat cggagtctca
    28981 ttaagagccg gtgatttcta tgagatcatt taccgcggac tctcgctgaa tgtattcgaa
    29041 gacagaccac ttgcttatga tacaggaatt gacggagtga gagtaactta caatcataat
    29101 ttcggaagta agaagaatcc ttttaaattc gaagcgcagg tactcggcgg agatataaaa
    29161 tatattgatt atctggatcc ggacagagaa gaaagatata aagtcagagg agtatttaca
    29221 gaattttcac ctatcaaacc cctgactgtc ggatttaatt ttatttattc tattggtgaa
    29281 cttcctgagc aggatctcat aactactgta acttcaaacg tacctgagct cagcgcaaca
    29341 tatactacac aggattttga attttatgca ggttatgctt ttaaatcttc catcattact
    29401 ccgaatgaaa tttatcccga tcctaataca gctcttggag acggcgttta ttcttcctta
    29461 tcatattctt tcaataacat cggacttact ttagattata aaaattacag atttgacgta
    29521 actcttcctg ataacagaag taataccaga ccttcaagaa tgcttcctta tcagaatccg
    29581 cctactgcaa taagggaaca tacttcagtt ttaatttcca gaaatcccca tgtcgtagat
    29641 ttcaatgatg aggtcggagg acagatcgat cttgtgtatg caccgaatga taagttgtca
    29701 tttaatttaa acggtagcat tgcatcgaga cattatcaat atacagatac agattcaggt
    29761 gcaacagttt cttatgaaag agtggacaga ggcgacagct ttctgccgga tcttgatgat
    29821 gctttctcac ctttctggga attttatctc gaaggtgaaa attatgcaac tgaagatcta
    29881 tactacagac ttgcatttgc aagacagagc agcgttcttt acaatcaact taatccgaat
    29941 tcttctgaaa aattacttac atctaccgtt cccgcagagt ttaaatatag tttttataaa
    30001 aacctgacat ttaagatcaa tgctgaaatg cagtgggttc ataattctat ccgtgtgagt
    30061 gaagataaaa attttactaa tcagtatttt gctttatcct ttataagagc tcctgacatt
    30121 accctgactg tcggttctga atttacaagt gatgaagaag aacctacagg taaatcttca
    30181 tggtttatag gtgaagcgac ttatcagatc aatcagtcaa atacagtagg agttggattc
    30241 ggtaccgaaa gaggtggact taaatgcgct aacggtatct gccgttttgt aaatccgttt
    30301 tcaggattca gactttctgt agctacacaa ttttaattat agctaaatta tatttatata
    30361 attatataag gagtaatatc atgtcattaa aaaaattttc atcatcatta atatttttat
    30421 ccctgataat tttttcaggt ctcttatttc agtcatgtga aagtaatgat tcaccgacta
    30481 caaataatac acctgcttcg actgttcctg ttccgcagtc acctgaaaat aattctacag
    30541 tatctactct tacaccttta tttaactggg caggagtcaa tgtagcagag acttacaaac
    30601 ttcaggtttc agaaaataat acattcacaa gtattgtatt agatgttgac gggatcactt
    30661 ctactttttt ttcttcggta ggaagttcac ttcaggacag cactacatat tactggagag
    30721 tcaaaggcct gaatacctct gacacaacag actggtcgat tgtatatgga tttgcaattt
    30781 cacttgagtc aataaatccg accactaaag tattagtcga acttttcaca aacacaagct
    30841 gcattccatg cgtcgaagct aatcagtatc ttgacggaat caacagcaac ctcggagtta
    30901 cgatcaatga tccgaatgtg ctgattctca gagttcatac tacattatat gcaggcgatc
    30961 ctttttattt atataatact gcagataaca atgcgagaat gatagtatat ccgggcacgc
    31021 cgacttcaaa tccgagagga tatctgctgg gtgcattcat gaataattac agttcatccg
    31081 catggacaaa tgtattgaat cagaaacttt cagagaccag atcttttgct gtcaatctga
    31141 ctaatgtcta tgattcagtt tcaagaaacg gtaatattac tgtaaagatc aaacaggttt
    31201 cgggtccgac ttacaatgac ctcgtatatc acattgcttt atcggaaagt gaaattcaat
    31261 ataacgcccc aaacggcgag acggaatttg aaaatacatt ccgagatctc gtcactcctc
    31321 caagcggtca gccgatgaat attgctacag gtcagacaaa cagttatgat cacacttata
    31381 cagtcgatag tgcaatcgat caggatcatg cttacataac agtattcgca cagaggacaa
    31441 ctgcagtcag cggtatcagg gaagtaatgg gagcagagag agtaaagata aagtaagaat
    31501 atgtttttta aaacattaaa aatttttaat gagacatggc tttagggtta tgtctcattt
    31561 tttttataat tgtcttaacc atgatttacg gaattaaagg atttatagga tttgtatttc
    31621 tgcaatttaa attattatta ttattaatcc cggaaatctt ctaatcttaa ataaatgcag
    31681 gttcagacta tacattccgt caatttaaga attgattcaa aaaattaata aatctaattt
    31741 tacaacattc attcaaaaaa taattaacta tcatgtttag aaaatttaca gtttttcttt
    31801 tatttacatt ttttatttcc acttattcat attcccaaaa agtggaaacc tctgccgaaa
    31861 aactggctat agaacttgcc aatcctgtcg ctgatctgat cagcgtaccg tttcagaata
    31921 atactgtctg gggaatcggc gaatacaacg gctctcagaa tgttttaaat tttcagccgg
    31981 tgataccgat tgcaatttca aaaaacatta atataatcaa tagaatcata gtcccgatgg
    32041 ttacacagta taacatcacc ggacccggac aaaatcaaaa ttctcttgga gatatagttt
    32101 acagtatttt tatttctcct aactcaaaaa aactaatctg ggggataggt cctgtaatgt
    32161 ctattccgac agctacaaat aaacttactg gttcgggaag attcagtgtc ggaccaacag
    32221 gcgttatttt aaaacagtca aatggatgga cattcggtgc tttgataaat caatactggt
    32281 cagtcggcgg taatccggac agaggaaatc agactgtagg atatctgcag ccgttcctga
    32341 attataactg gaaaggcggc ggcggtattt ccatgaatgc agaaatgaca caaaactgga
    32401 agatcaagag gacacagact tttatgaaca ttcttattac aggtattacc aaattaggaa
    32461 aattcccggt atcacttggt gtcggaccaa gaattccaat tactgcttca cctgaagcaa
    32521 aaggcttctt tggtatcaga gcagtagcaa cttttatatt tccaaagtaa tcattaattg
    32581 cctcaagcct cttagtctca aagactcgaa gttcaagttc agtaaaaatt ttttccttcg
    32641 agtcttctat ataacttgaa agttattatt gaaaaaacat agttcaaaat atatccggat
    32701 acttagaggc aaagttataa attttgtttt ctgtcagtct gtgagtttga aaacgagcat
    32761 caatttttat aaaggaaatt attttcattt attataaact ttttgaattt attaattttt
    32821 atataataaa ttaaatatgg aaaatatttt aaaagataaa acagtcagca ggttaaaaat
    32881 agacggtgtg agaccgatag atctttcact tgatctgcgg gaagaaatat caaaactgaa
    32941 aagtgaaatg aatgcggtca tccttgctca ttattatcag gagtctgaaa tacaggatct
    33001 tgctgatttt attggtgaca gtctgcagct ttcacagcag gcagcaaaga cagaggcgga
    33061 tgtgatagtc tttgccggag tgcattttat ggcggagact gcaaagatat tgaatcctga
    33121 caaacttgtt atacttcctg atctgaatgc cggatgttcg ttagccgaag gctgccctgc
    33181 tccgatgcta atgaaattta aggaaaaata tcctgaccat attgtcataa gttatataaa
    33241 ctgttcagcg gaagtcaaag caatttcgga tataatttgt acttcttcaa atgctgtaaa
    33301 gattatcgat caaattcctg ttgaacagaa aataattttt gcacctgaca gaaacctcgg
    33361 cagatatgta attaaaaaat ccggcagaga tatggtgctc tggcagggtg cctgtattgt
    33421 gcatgagaca ttcagcgaaa aaagaattaa caaattaaaa tttgaaaatc ctgatgcatt
    33481 attgattgct catcctgagt gtgaggaagc tgttttaagt aatgcagatt ttatcggatc
    33541 gacgagcgga ttattgaatt tcgtaactga gtcagaaaag acggaattca tagtagctac
    33601 tgaacccggg atcatccatc agatgctttt aaaaaaccca tctaagaaat ttatacctgc
    33661 accgtcagag tcaggttgta attgcaacga gtgtccgtat atgaaactta atacactgga
    33721 aaagttatac ctctgcatga gagacagaaa gcctgagatt gtaatggatg aggatattct
    33781 aaagaaagca ttgctgccat tggaaagaat gctggagatg agtaaatagt tggttttcct
    33841 ttacctcgaa ggctcctgga ctcaaagact caaaggctca aagaaaaaaa taaaattcct
    33901 catcgcaaaa aacgagatat cttgttgcaa tagataataa atttttacgc cacaatgagg
    33961 attaaacact gatagattta aaatattctt ctttgagcct ttgagttttg atgtaaatca
    34021 aattttcaaa atacactttt attctttaag caattcttcc tttgcagaga taagttcttt
    34081 tttcatatct tcatttactt ccggatattt cagatcaaga ttactcagat atttatggat
    34141 aacagcagtg atgagaattc tagtatacca tttattatca gcagggataa tataccatgg
    34201 ggaattttct tttgaagtat ttgaaatagc ttcttcataa gctttctgat agtcatccca
    34261 gtattttctt tcatttacat cgttcataga aaatttccag ttcttttcag gcttatcaat
    34321 ccttttaaaa aatctttttt tctgttcctc ttttgataaa tgcagataga attttatgat
    34381 aactgtacca ttttgcctga ccgttttttc aaaatgattt atctgttcaa aacgctgact
    34441 ccagaaatta tcatcaatat ctttgactga atttattccc ggaatttttt cattcatgac
    34501 atattcagga tgaactcttg tgacaagtac attttcataa tgcgacctgt tgaaaatagt
    34561 tatgtttcct gcccttggaa gatcgatata atgtctccat aaataatcat gttccagctc
    34621 gagtgtattt ggtgctttat aactatgtac aatcactccc tgcggattaa aacccgacat
    34681 aatatgtttt atagatccat ccttaccggc agcatccatt gcctgaaaaa tgatcaacac
    34741 gctgtatttg ttgtcagcat aaagtacatc ctgaatcgct gacagctttt cctttccgga
    34801 ttcgagaatt tccagcgatg attctttgtc aagcttatca cctttatatt ctgtatccca
    34861 ttttgaaaga tctactttct tatcaggttt tgcaaaatat ttatttatat ctatatcaaa
    34921 ttcttttaac ataagtgatt tttggttaat tgaaagtttt tatctggata ctgtgactta
    34981 tattaaatac tgcacatcca aacaaaattc tgattagaca aaataatctt tacaaaatta
    35041 aatagaaagt tgtagataaa ctctcagcac tacaaaaaaa tacattctaa tttaaagtaa
    35101 aaggaattaa attggataaa caaaatttat atctttataa aaaaataaaa ttcttagcat
    35161 gaaaaaaaca ttacttattt tatttatttc acttacacct gttattaacc tgtattctca
    35221 ggaaaatcct cagcaagttt atcgtaacgg aatcgaaaag ttttttaatt ctgattttga
    35281 tttagctatc acatatttta cagattatgt aaaaacagaa acacaggcat acgaagcgta
    35341 taattacaga ggattatgtt atcttgctca gaaaaattat gcaaaagcca aagaggattt
    35401 ctctactgca gtacatttaa atataaaata tagtcagggt tttgtaaaca gggcaaatat
    35461 ttttgcactg gagggaaatt atgaagcagc actcagggat tacaataatg ccattttagc
    35521 tgacagggca aatctcgaaa tttattacgg attgagtttg ctgtatatca acagcaaaga
    35581 attcaacaaa gcactgatag aaatgaaaac agcggctgcc attgatccaa aaaatgcaag
    35641 agtatatatg aacacagcta ttgtaaacat gcttatagga gatacattaa agatatttga
    35701 aaatgtcgat gaagcacttt actgggattc ttcatattta tatacaaaca ttaacaagtc
    35761 tctgattttc acaattgcag acagatttca atatgccatt caagtactga ctaatgtaat
    35821 ttcctccaat ccaaacagtt atcttgcata ttttaacagg ggagtggttt tttacatgat
    35881 gggaaatttt tccgaagcaa tttcaaactt caacagatca aaagatcttt ataaaggtgg
    35941 agatcagagg atcattaatc tgatcaacaa gatagttcgg atttcggaat ttaacagtta
    36001 gcttaaaatt gtatactgta aattatgacc agcacagtta ttttgattca aacaatttcc
    36061 atatctaata acagaatctt gaaaagttca gactggataa aatcttgaaa aaaatattac
    36121 ttttattaat catttccata tcaacactaa caaatcttta ttctcaggaa aatcctcaat
    36181 caatttatcg taacggaatc gaaaagtatt ataattcgga ttttgatgaa gcaatcgttt
    36241 attttacaga ttatttaaaa atagaaacac aggcttatga agcatttaat tacagaggtt
    36301 tgtgttatct ggctcagaag aattatcaaa acgccaaaaa tgatttcaca tcggctgtac
    36361 atctaaataa atcatttagt cagggttatg taaacagagc aaatatcaat gctcttgagg
    36421 gagattatga tgcagcactg agagattata gagatgcaga agaagttgac agagcaaatc
    36481 tggaaattta ttacgggctt ggtcttcttt ttatgaatag caaggaattc aacaaagctg
    36541 agatcgagct gaaaaaggca gcagccattg atccaaaaaa ttccagagta tatatgaata
    36601 aagcaattgt taatatgctt ttaggtgata cattaaatat ttttgaaaat gttgatcaat
    36661 ctctatactg ggattcaact tatttattgg cagacctgaa taaatcacta atatttacaa
    36721 tagctgaccg ttttaaaaat gtagtcgaga tactgaccga gaaaatttcg gattatccta
    36781 atagttatct ggcatatttt aaccggggag tagtttatta tatgatcgga aattatacca
    36841 atgctattca agattttgag agatcaaaaa ttctttataa aggaaaagat cagaggatca
    36901 taaatcttat agataagata ttgcgaatat cgaaatttaa cagctagctt aaaattgtat
    36961 actgaaattt tagatcagca tagttatttt aaaaccaatc ttatttcaaa tctaaaaaca
    37021 gaaacttgag aaaacccggt cagatcattg cccttggagg aggaatgttt tcaatggaat
    37081 ccgacaacgg aatgcttgat aaatatatat taaaccttgt cccgaaagaa aagccgaaga
    37141 tctgttttat gggaacggca agcaatgacg gtatagaata cagagacatg ttctataatt
    37201 tttttaataa gatgaagtgc gaaccttctc atctctcatt cctcgaccag cctaaaaaca
    37261 tagaaaagtt tattttaagt caggatataa ttcatgtagg cggcggaaat acttcattaa
    37321 ttattgatac ctggaaaaaa ttcggagttg ataaaattat gagaaaagca tggagatcag
    37381 gagtgatact tacaggaatg agtgcaggag cgatctgctg gtttcaggac ggaattacaa
    37441 atccgtcacc ggggaaactt aacagactgc cttgtctcgg attgctaaag ggaagcttct
    37501 gtccacacta tgatgacaag gtcgaattga aaagatcatt tcacaaactt attctcgacg
    37561 gagtaattgc tgacgggatc ggtgctgaag acggtgctgc tgtacattat ctggatgata
    37621 aaattatcag agtcgtcact tcaagacccg gagtcacagc ttattccgta aagaaagtta
    37681 aaaataaaat tgtagaaacc gcgcttgatt cagtatattt aggaaaggaa tctgatattg
    37741 aacatgttca gaataattca ccaattgaga tatttacaac tgctatttca tatattgaaa
    37801 aaataaatga acatgacatt aaaggtattt gtgaacttat gtctgacgat catatattca
    37861 ttgactcact tggaatggtg ataaaaggta aaaaggaaat gtctaatgcc tggaagatgt
    37921 ttttaaaatg gtttcctgac tataatatta caattatgaa tactgttttg actgatgata
    37981 cagtcggaat atttggaata gcttccggaa catttaattc ggatgatata gatatttcag
    38041 ataattttga aattccaggt gcctggcgcg caaaggtaaa agataaacaa attacagaat
    38101 ggcaggctat agctgataat gaagcggtaa gagatattat aaaaagtaac ggagtgaagt
    38161 caatgttcag atcaaaaatg aaaagcaaat cattttaatt attcagtttg aaatgaaggt
    38221 tgataaaaaa gttcacggat aaaatgaaaa aaattatatt ttgacagaaa aagaaaattt
    38281 tgcaaaagag ctgaaaagaa gtttagatga agatacgttt gtaaaaatac agttcggaaa
    38341 atacagaggt gatgacaaag attttcaaaa gataatagtt acgaaattaa ttttaaaaga
    38401 aggcgaacat ctttcattca attttaaata caatagtaag gacgttgtaa aaaattttga
    38461 aattgaaaaa ggaatatcac ttgcagatga aattaccgga aaagattttc tgagcgctac
    38521 tttgtttaca acagagaatg attttacgat agaccattca aagaaaagag ttgcgtcatt
    38581 tcacaaaaaa aaaccttctt taaaaaatcc ggctgtatct gtacataaca gagagaaggc
    38641 aagaaatata gatcctgaag caaaatattt ttatctgttg ggaattacta cagaagaagg
    38701 taaagtaaaa tcgggaatgt atgataaatt caaacaggtt gataaattca tagagacaat
    38761 ggattctctg ttcagagctt cagcactttc tgaaaaagat gaaattatta tcactgacat
    38821 gggttcgggt aaaagttatc ttacatttgc attgtatgat cattttaaaa atacattaaa
    38881 taaaaaagta aatattagag gtattgagca aaggcaggaa cttacagatc tgtcaaacgg
    38941 cattgcaaaa aaatgcgggt tcaatgaaaa taacggattg gaatttctaa cagggagtat
    39001 tgaagaagcc gatctgaata atacaaatat tgcagtggca ttacatgcat gcgatacagc
    39061 gactgacgat gcaataaaaa aagcagtaag agcaaaggca gaaattattg tattagcacc
    39121 atgctgtcag aaatatgtta gaaaaaaaat agtagttccg gaaagactta aaggaatctt
    39181 cggatacggc attcatgagg agcgtctggc agtaatgctg acggacggac ttcgcgcgat
    39241 gacacttgaa aatctcggat atgatgtaaa agtatttgaa tttatttctt cagagcatac
    39301 agcaagaaat acaatgatca ctgctgtaaa aaatgaattg aaagatatag gtaattcaaa
    39361 agatattgaa tcagttaaaa aagaattcgg gattgatgat ttctatcttg ataaaatttt
    39421 aaacgcatga aatatttaaa tatatgtaat gaaaaaaatt aacagagaaa taatattaaa
    39481 agcaaagaag aacggatttt ctgataagca gttgtctgta atgcttggca catctgaaga
    39541 caatataaga aaatatagat ttgccaacga tattatccct gtttataaaa ctgtagatac
    39601 atgtgcagca gagtttgaaa caagtactcc gtatcactat tccacttatg aaaaatacaa
    39661 cgaaaacttt tcatcagaca aagaaaaaat aattatatta ggcggcggac caaacaggat
    39721 agggcaggga attgaatttg attactgctg tgtaagatgt gtgaaagcat tgcgggaaga
    39781 aggttttgaa actataatga tcaactgtaa tcctgaaacg gtctctacag attatgatat
    39841 cactgataaa cttttctttg agcctctgac tactgaagac gtactgaata tagttcatta
    39901 cgaaaaaaat gtgaagggaa ttgtcgtaag ttttggagga cagacacctc taaagatctc
    39961 cagggagctt gaaaaaaacg gactgaaaat cctcggcaca tcttctaaaa atattgacat
    40021 agctgaagac agagaaaaat tcggaaagct tttggataag ctcagcatac caaagcctga
    40081 atacggtaca tgcactaata ctgaaactgc actgaagaca gcaaatcgta tcggatatcc
    40141 agtactactc agaccatctt atgtattagg aggcagggca atgcagatag tttatagtga
    40201 tgggcagata gagaaatttt tccgtgatga agctgaaatt tccaaagaac atccggtatt
    40261 gattgataag tttttagagg aagcaagaga gattgatgta gatgcgatat gtgacggcga
    40321 agatgtttac attgtgggta taatgcagca catagaggag gcgggtattc actcaggaga
    40381 cagcacttcc atacttccgc caatctcgat cagcaaaaaa aatcttgacg aaataaaaaa
    40441 atacacaaag aaattagctc ttgcattaaa tgttatcgga cttataaata ttcagtttgc
    40501 tgtaaaggat aaaatagttt atatacttga agcaaatccg agagcttcaa ggacagtacc
    40561 gtttgtcagc aagacaacag gtattccggt tgtaatgatc gccgcaaaaa ttatatccgg
    40621 taaaaagctg aaagatttta aactgaaaag ttttgaggaa atagaatata tcggagtgaa
    40681 ggaatccata tttccgtttc agaaatttcc aaaagtgaaa atgtttctcg gacctgaaat
    40741 gagatcgacc ggagaagtta tgggaatcgg cgcgacattc ggagaggcac tggcaaaggc
    40801 acaggaatca acaggtgcac cggtaccgac caggggaaat atttttatca gtgtaaatga
    40861 aaatgataag aaaccaaaaa caattgagat tataaaagaa tatacaaaac ttggatttgg
    40921 agtaattgca acttcaggta cttctgtttt tctaaatgaa aatggaatta aatccgaagc
    40981 agtatttaaa gtaaatgaag gcagacccaa tgtagtggac agaattaaaa acggcgagat
    41041 acagatcgtg ataaatacac ctcttggcgc tgattcaaga tttgatgaat atgctattgg
    41101 atggacagca gtacagcaaa agataccgtt cattactacc ttgtctgcag cacagtcttt
    41161 tgctgaagga atctcgagga taaaaagggg aaagaccagt gtaaaaagtc ttcagaatta
    41221 ttataagaaa taaaatttat gcaatagata tataaatttg aaaatcatat ttttgtacag
    41281 ttaaaaataa aatggactta gagaaaacaa agaacagtta taaatgggat ccggcatcgg
    41341 gagattattc aaaaagttat gtctcctatg aaagaaaaat atccaataaa aaacaatcaa
    41401 gaaaatcctt actaacgata atttttattt tagcaatcat agtcagtata gtagttattt
    41461 ttattgagtt ttcataaagt tttagaactc ccttcttatt aaaaattgca taaattccga
    41521 aagtaaacaa actttgcaat ccgataataa ataaattatt tgttgccgac tttcgttttt
    41581 atacaacatt aaaataaaat gatttaaaag ttataataaa ccggactgac taattctgtc
    41641 tgtaagaaat ttattctttt atgctgctct aaaattgtta ttcaaagtgt tctccctttc
    41701 cataccgatc taccggatct tgagtgaact actgatctga tactaatata aagaagcatc
    41761 agcatttgca aaggatacaa caaaatattc ataaaaatat tctgagaact aacaatagaa
    41821 actatgaccc tctgcagaat aattattaat attaagccaa tataatattc attgtaaaac
    41881 attaatatta atggcagtat atttgataaa acaatgaata gtatattaaa aataaacaga
    41941 aggggagacg atgctgaagc tggataaata tttttcgaaa atcccgatac tgcttcttta
    42001 aaactattat acattctgca gaaaacaaga tcattactta aaagcagtaa tgtattatat
    42061 ccgtttttct ttaaatattt tgctatctca atatcttcta ctatttcatt tttcacagcg
    42121 gagtgcccgc ctgacttttc gtatgtttct tttttccata aaatgaactg tccgttagcg
    42181 ccggctaggg atttgtactt cg
//
LOCUS       DAORVQ010000008        38326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_315_length_38326_cov_6.828251, whole genome shotgun sequence.
ACCESSION   DAORVQ010000008 DAORVQ010000000
VERSION     DAORVQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 38326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38326
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_315_length_38326_cov_6.828251"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLX80_01540"
     CDS             <1..709
                     /locus_tag="PLX80_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19508.1"
                     /translation="GDTDPENVILLEIEPEKQKTYIDFLCTEKYIGVKYVCLTKVIKE
                     GKKLYYENNGRKIPIDKIYNRVIYDELSKRKDIKYNFRFTEELDVKWIAHPNWFFKVS
                     KYSLPFLKSKYVPETKFLSDYSEFPADTENYILKPLYSFAGAGVIFDLTKENLDAIPE
                     EDRSNFILQKKINYEPLIETFDDPAKAEIRLLYVWNDKPILINNLVRLSKGKMMGVDF
                     NKEKTWVGSSIAYFRKK"
     gene            1054..>1346
                     /locus_tag="PLX80_01545"
     CDS             1054..>1346
                     /locus_tag="PLX80_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19509.1"
                     /translation="MRNYDYSSEGYYFITICTDERKCFFGYIENGIMILNDIGKIANE
                     YLLEIPNHFKHALLDEFTIMPNHIHLILGLNYNIPSVRTRHGVSQQDGISQQD"
     assembly_gap    1347..1446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1447..1855
                     /locus_tag="PLX80_01550"
     CDS             <1447..1855
                     /locus_tag="PLX80_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY19510.1"
                     /translation="ESKEDDVRTRHGVSQQDGVSQQDGVSQQDGISQQDIESKEDDVR
                     TRHGVSQQDGISQQEQSTNKFGKLNKNSVSVIINQFKSSVKRWCNKNGHENFKWQPRF
                     HDHIIRNEGEHKRIKQYIIDNPSNWKEDKLSDK"
     gene            complement(1976..4426)
                     /locus_tag="PLX80_01555"
     CDS             complement(1976..4426)
                     /locus_tag="PLX80_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY19511.1"
                     /translation="MTNQIILLFVVFELFAMFPKFSSAEKNTFTPKIPNTGLSGKISD
                     KKTGEPMPGVSINFPDLRTGTFSGSDGIYKINELPQSKVLIRVSMIGYKLISEEIDLS
                     VVSVKNFEMEESAVEINEIIVTGLSKASELLKIPTPVITVSPAQLIQSSATNIIDALS
                     INPGISQITTGSGISKPVIRGLGFNRVLVVKNGIRQEEQQWGDEHGVEIDEYSVNSVE
                     ILKGPASLSFGSDAMAGVINLISAPALPEGEISGSLLSNYQTNNGLFGITGNIAGNKK
                     GFIWDIRYSNKLARAYQNKYDGYVFNSGFKENGLSGMLGLNKSWGYSYLYFSTYSLTP
                     GIVEGDRDSVTGMFVKSVAENDTTEGTEVVNNEELTSYDLNTPFQKTYHYEVTLNNNF
                     ILGNASLSTVLGWQQNQRQEFGDVFEQNQYGLYFLLNSYNLEARYNLPEVNKYNFSFG
                     TNVMYQTSKNKGTEYLIPDYNLSDIGFLGIVNKSFSKLNISGGLRFDLRSMQGKDLYT
                     NSAGVIAGPDDANSIPLFSDFNNNYSGISGSIGAVYQFNENFYSKINLQKGFRGPNIA
                     ELSSNGVHEGTFNYIIGVPDLKSENSLQLDYTFGINTLNAAAEADLFYNVIDNYIYLE
                     KLQSISGSDSITDGYSTYKYASGNAHLFGGEFTLDIHPDPFDWLHFENTFSYVQSVQT
                     NQPDSTKYLPQTPPARITTVLRADINNFTCNFQNSYFQIGMENYFAQNKFFAAFGTET
                     ATPGYTLFNMSFGADVFINNNNLFSVLMSVNNLTDIAYQSHLSRLKYGDVNEVTGRKG
                     VYNMGRNFNFKLVVPMGF"
     gene            5140..5541
                     /locus_tag="PLX80_01560"
     CDS             5140..5541
                     /locus_tag="PLX80_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017000756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19512.1"
                     /translation="MNGVIHHIEIYVSDLNKSTAFWQWLLTEKFSYKVFQKWDSGISF
                     KFESSYIVFVQTEKKYAANSYNRKNTGLNHLAFHCTSRNFVDDLTIDLKYRNIEILYP
                     EKHPYAGGENYYAVFFEDPDRIKVEVVVSEN"
     gene            5761..6882
                     /locus_tag="PLX80_01565"
     CDS             5761..6882
                     /locus_tag="PLX80_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19513.1"
                     /translation="MNNRHITFFLLLVSIIFIYGWGANGHKIINRRSTFSFPSPLTFL
                     NWADLLAAHSSDADYRRSSDPNEAPRHFIDIDNYPEFIATGRIPQDLDSLIAIHGSTF
                     VYDNGILPFSIMATADSIKKYFQLQNYPKAMLHAADLGHYVGDGHNPLHITRNYNGQY
                     TNQNGVHSRYETQLINRDSGNIIYSFESAVYIPNINQFVFDFLYNNYTYVDSVLKYDS
                     IANAMAPGYGTAYYQRYWQLAGNFTIMLFNNASHFISSLIYTSWVNAGGAVSISATET
                     SLPEKPFLAQNYPNPFNPSTKIKFGIPDLHHYNSGGSHTKLTIYDISGKEITNLINQQ
                     LQPGTYEVVWNAADHPSGTYFYKLSAGDYSDSKRMFLLK"
     gene            7198..7875
                     /locus_tag="PLX80_01570"
     CDS             7198..7875
                     /locus_tag="PLX80_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19514.1"
                     /translation="MKTFTLKFVLTILFLSLIQSISFAQPYKINLYNSYVFDDGIEFV
                     TDNNRYINGTMNGGYQWGIGFEYILQNSVGIELMYNRQNTNFPITYSSTIDTTRSFDV
                     DVNYILLGSNKYIPLQNKMIKPFGGVAIGLAILNNQNPLPGGESSSTKFAWSARLGSD
                     FMFSPSVGIRIQGQVQSAVQDVGGGLYLGTGGVSTGVNTESSMYQFGLGGSLIIQFGA
                     PKTNRSR"
     gene            7902..8474
                     /locus_tag="PLX80_01575"
     CDS             7902..8474
                     /locus_tag="PLX80_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HQY19515.1"
                     /translation="MLHTMLKKWWVILIQGILLFILGIFIFNNPAEVLAGISLWIGIL
                     LMVTGVIGLFGWIFSSSGDRDTWVLIWSLLTAVFGVIVLMNMLAAMKAVTFIFGIWVL
                     ITGFNLTSSGWSMKNENGLGWLLFIIGFLSIFAGFMMIFNISAGAVGVATILGIQVLL
                     TGIAMILLSFVKKALSNKVKGKIEELKSSI"
     gene            8775..9881
                     /locus_tag="PLX80_01580"
     CDS             8775..9881
                     /locus_tag="PLX80_01580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQY19516.1"
                     /translation="MIFIFISSCGQLNKDQEKSKFKNKNPEHSDKKSSVTNQISKDNL
                     ITIIAAGDVMPGSNYPSGYSLPPDDGKYLFDSVKNFTGSADVNFCNLEGTLLNSGGKP
                     KECNTPHNCVSFRIPEHYAGYLKDAGFDLVSTANNHSNDMGEAGKISTINTLKNYDIN
                     FAGYNDHPTVVFEKNGVTFGFAAFAPNKGTQDLNNISNAVKVILDLRKKCKILIVSFH
                     GGAEGYSAQRVSGDKEIFLGEDRGNVYEFAHSVIDAGADIVIGHGPHVPRAMELYNGK
                     LIAYSLGNFCTYGKFSLSGPQGIAPLLKVYLDDKGDFVKGNIYSFKQVRRGMPVPDEE
                     LNAVNLIKSLTEKDFPGGDLLISDEGEITVRRSE"
     gene            9980..10753
                     /locus_tag="PLX80_01585"
     CDS             9980..10753
                     /locus_tag="PLX80_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19517.1"
                     /translation="MKNILVIPILIIFLFSEKNYSQSYDSGDGFYSGFGTSISSYWGG
                     YFGEAYQMRVLSSYDYYDDINYSNDNYDDYSIWSPYTISFTFGKHFSDNLSVEAESDF
                     LFHLNGRVDPQFVTAEIGDQYFQDRNSYSTLFAIPISLSIKLSMEDESGGAFIKIGPA
                     WQYTSEQYDRIRNYYSYEYYNSYSYDVYLYNVSKDEWLFGFKTGMGLKYYMSDFTYAV
                     TELEYAYFNINPTNKTALALDRAPEAQLFSLSAKVFFDF"
     gene            complement(10809..11375)
                     /locus_tag="PLX80_01590"
     CDS             complement(10809..11375)
                     /locus_tag="PLX80_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY19518.1"
                     /translation="MKTSFFTFVFLISSILLLPLKSHSQEKTEYVCYPCGSGCDKEIH
                     HSQGICSDCNMPLIEKSKVIFKSIDTPEFLSKLENEKGFILLDVRSAEEFNGKGKGPR
                     SLHGYKHFKNAVNLSSDILEENIGEIMKYKDSEVIVYCSHSRRSPFSSQILTDAGFTN
                     VTNYSGGIAEFVEEYLINSNSGKDFIVK"
     gene            complement(11503..13116)
                     /locus_tag="PLX80_01595"
     CDS             complement(11503..13116)
                     /locus_tag="PLX80_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19519.1"
                     /translation="MRNYIFLLIIIFCSSEIYSQYPSFRIHPSLNNQIEPTLVVHPLN
                     PDIMFASAFTIRLSFKSEGIYVTTDGGVTWTGNDTCTGQPLSNHGGDPGPIIDKDGNF
                     IITHQGGFILGMYSNTSTDMGQTWTNNLQIAGNDQDKGNPATDNNQMSPYYGRTFLVW
                     TRFTNPFPIVTSYSNNSGLNWTSVSQVNNSLPQRQSNGPTMVIGSDGKNYVVWASSLL
                     SSPFNEKNMGFATSTNGGDSWSVNENIYACNGVKSSSLQPWNIRVNGFPALEIDKTGG
                     SRNGWLYIVSSQVNFSPAGSDPDVVFHRSSDGGITWSSGIRVNQDPLNNGKNQVFPAL
                     TIDESGGINVIYMDNRNSPDSAQIYLSRSTNGGDNWTDYIVSDHKFLPKSVSGAGAGN
                     QGDNIGITNTNNKLFPVWNDDITGVYQVWMAPIDLATIGINQISTEIPNGFYMSQNFP
                     NPFNPSTKIKIDIPSESNGINKFRLVVYDINGKEVSTLVNSNLTTGTYEVTFNAFDQS
                     GYELPGGVYFYSLFAGDRLVESKKMVLLK"
     gene            complement(13328..15448)
                     /locus_tag="PLX80_01600"
     CDS             complement(13328..15448)
                     /locus_tag="PLX80_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY19520.1"
                     /translation="MKNLIKKSLVFFLITVGTVMAQESKDTTDYETDEIIISATRTEQ
                     RLIDIPFSVQRVDQSSWKSSRKMGVNDVLTTVPGLILQPRYGNHDVRVSIRGFGSRSN
                     TGVRGVRILLDGIPESEPDGQTRLEALDFTAIGRIEIAKGNLSSLYTNAPGGVINFFT
                     DKYFPTSFVLTDNEFGSYDLRKNGFKVGVKTDRERFMATYSYENYNGYRAHNNEYQTR
                     FNSIYEADISDNEKILINGYYVSGLIKLPGSLTLDQYNANDLQANARDESRDAKRLTD
                     KGRLGITYNSYFDKEKKNFIEVTGYGTIKEFVRTNATYRIFNRYGLGATFRFINKSTI
                     ANRENEFSVGSDLLYQTGPIGEFNNIGGVKGDDLIALTDETLSNVGFYFLNQYPVLPG
                     KLSLLITGRYDRVVTAATDLLAGFRDTSRLFDNFTPKFALNFLLTPQMSIYGSFGFGF
                     DVPANNELDNYPFSSDGGLKLINPDLNPQQTVNYELGYKGEIPNRGSKIFRNTFMELT
                     MYSTKIDNAIIPFTVDGNVFFRNAAQVLRTGIEVGLNTQIAKGFDFSTSYTYSNFKYE
                     EYTARSISGTGQISEEDYTGNTEPANPDNYFNANLSYTYDFNENYNVFIKNTNQYVGS
                     MFVSDKNVDSLKTDPYYLMGVQFGADLTFGQFRFLTYLGMNNIFNKKYVAFVQINSDR
                     QEYYESGPIGNIFGGLNISYLFDR"
     gene            complement(15821..16459)
                     /locus_tag="PLX80_01605"
     CDS             complement(15821..16459)
                     /locus_tag="PLX80_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19521.1"
                     /translation="MKKISIIVFLIVSVICSEANAQKTGKGFYIGTFQYSTTFNIGRA
                     HDYVSNVSFWGASFGIKKFISQSVTVGFNISHNVLSNKYDDGTTEVQNGLITGPQARY
                     LNYTPMLFSIGYVFNKGYMVRVFPYIQANVGAYYIWERLQLGANFKSNNNLHFGAGPE
                     AGIIFYIGKRFGINVNANYNYALGAGNPIVGRGDDNSFTFFNANVGISYINK"
     gene            complement(16530..17144)
                     /locus_tag="PLX80_01610"
     CDS             complement(16530..17144)
                     /locus_tag="PLX80_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQY19522.1"
                     /translation="MKNQMSRTLRFIRIFSLIILMTSPVVISSCYVDYGLATNNIPIV
                     ASFFNPGYNFNDVTKYYFLDTVVQINGANVTRVYDNTIKNTTINNLNALGWTRITDSS
                     MARDPGVVIVGNVVTTTSYTVTENNGCWGGVWSYSSCYSYDTEYEYSTGTIGIVIVDV
                     KVLNGESLPIQWIGLSNGVMSSGGNVSQKIIDSINKAFTQSTYL"
     gene            complement(17293..17931)
                     /locus_tag="PLX80_01615"
     CDS             complement(17293..17931)
                     /locus_tag="PLX80_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQY19523.1"
                     /translation="MNKIKLFALTVFLTVFIFSDSYSQNMTGKGAYLASLEYNATFNM
                     GQASDYLSTPGFWGGSMEIKSFVQKNISFGILIGNNVISEEQENGSTEVKNGTITGPQ
                     ARFINYAPVMATVGYFFNKGKSKTVPYIQANIGSYYVQQRLQVGANIIDNDNWHFGIG
                     PQVGVLFNVGQNVAIVLDARYNYAFSSGEPIGNTDNNSYSFINANIGLAYTQ"
     gene            complement(17992..18597)
                     /locus_tag="PLX80_01620"
     CDS             complement(17992..18597)
                     /locus_tag="PLX80_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQY19524.1"
                     /translation="MKIQNSLKLFRNLLFALIIICPVIFSSCYTDYTLETDNYDVVGS
                     FYNPDYNFNNVNDYYLIDSVIHRDGGTITTDYDNTILSTTIANLNALGWRRVTDSTGS
                     GVVVVGTFVTSTTYVVNTGSGCWYDYWGYSWCYPYYGNTYDYTTGSVGVVISDLRNSS
                     ASNLPIEWIGVGNGLIGQGGNTTSRITTSINQAFAQSPYLK"
     gene            18885..20642
                     /locus_tag="PLX80_01625"
     CDS             18885..20642
                     /locus_tag="PLX80_01625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY19525.1"
                     /translation="MQNKKNYLKLFSLFIMISFIAVIACSSKKKQVVAEVGDEKIYLD
                     DYETQYMKTVNNIDSAKSSNMEKRTEFLDLLIKYKLKVKDARERGLLTSEDIQNDLKQ
                     YKENFLTSFLIDKDIVEPMIKDLYDKKEYEVRASHILINLPMQNMSPEDSIAAYTKAS
                     EVLAKLKDGVDFSVVAADYSDDNSAKQNGGDLYYFTAGMTVPEFEDALYELKVGEYTK
                     EPVRTNFGLHIVKLTDKKKRNEGIKASHILIQDQKDSLGNTIDSIGTYNKAKETLSRI
                     KNGEDFGKVATEVSQDPGSAPKGGDLGFFDRRRMIQPFDSVAFMLKVDEVSDLVKTPY
                     GWHIIKVTEIKPYTSFEKQKETLKGDYKKGPGFKAEYTKYIDKLIKEFKYEVNNEGLN
                     FLISKFDSNKTLSSFNIDSMFNADERAKIIATYNGGDVKVNDFVVYITKNRDAQASLA
                     NSETLRKVITNTGEMPLLYKKAIKENIEDDPLYVEQLTEYENGLLSFKVDQEELWRKI
                     KINPEDLESYYNSHKDNYSYTDSNEVKVKPFEEVKSEISNTIQQDKFKELEKEYIENL
                     KAKYPVKINAAILIEAFKE"
     gene            20649..21926
                     /locus_tag="PLX80_01630"
     CDS             20649..21926
                     /locus_tag="PLX80_01630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY19526.1"
                     /translation="MLKVIFIIPLFLILSSALFAQPEGDRIIAVVGKEIITESDFQYQ
                     LQLYARQNQLADISPYMVQQIFQSILTNKIILAKADQDSIFISEEELNRELENRIKNL
                     IGQVGSAEKLEEMYSMSMTKIKILLKEDLEKSMKTEKLKRQKFAGGMRVSDAEVKEFY
                     TSYGDSLPDVSEELEIAAIIMERKVSESEKKEAERIALQILDSIKNGTEFSDLARRNS
                     ADSLSAIEGGDLGIASKGTFVKEFEDAVFSLSPGEVSGIVETQFGLHIIKSNEKLGDK
                     VKAQHILIRFPKFESSDFETLNFLNDLRDKINKGEITFEDAAKQYSDDPQAKSNGGYA
                     GKVPAEQFDSATVEILNNLTDGQISEPIRIGSDTEYGYQIIKVISKIPPHKLTLKDDY
                     EKVKRYAENYKENKEMESWIEELRESIYVDIKM"
     gene            22096..24213
                     /locus_tag="PLX80_01635"
     CDS             22096..24213
                     /locus_tag="PLX80_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19527.1"
                     /translation="MNFSFSAEYGFVYAAIMLIVAGAVSYFYYKKTILKEPQKKFLTA
                     LRALSVFFIFILLLSPVMSFISNISPSPKNIFLIDNSESLLIENRNNLLKKIVTDEIK
                     GIGAGNSEDLYFLFSGDLIKEISADELNGITYEGINNFETDLSSPVSTLQEKLSAANI
                     STVNIVSDGMINEGGNPLKFARSMNVPFNYYLTGDTIQKNDVSIKNLYYNKTAFAEST
                     VPVKVQIASYNYNTNVNVSLYEEDKLIDTKTVAVNERDNLYDVSFNVFSAEDKIVKYR
                     IESEPLENEITLKNNFREFFIKFTDNKFKVLVISGGPSADNAFITEELKQVRNFETTV
                     LTQKSGGEFYEGGFPDINIFDTFILIGYPTAVSDQSILNRISAEIENKNSSLIFFAGR
                     NTDYSKLMSIEDKLSFKISSSSDNEELTGLSSVISTDNEIFSSLDQLRSVSSMPDIFK
                     TSSIFSSNPSADVFLLTTKNSIPALIIENTKSKKSAAFLAYGFYKWRLNKNNKGSDDL
                     FRNILSNIIIAITDKEAQKHFSIETTKSVYSKYEDVKFNASILNHEVNGNEKILVKIS
                     GNGIDKETDLIRLNDNQYSGKINIPEDGNYEYTAGLYDDNVLVENILGRFSVGENNFE
                     FSETRADNSVLKILSLETGGQNLTGKSKSEIDDILTEMNGKSEYEFKTARNLELNVNP
                     YYLALLIFLLCLEWFLRKRNNLP"
     gene            24646..25920
                     /locus_tag="PLX80_01640"
     CDS             24646..25920
                     /locus_tag="PLX80_01640"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY19528.1"
                     /translation="MTVLLSCAVEAVANDDYWLIQSKQTSQNLSSCEFTDTLNGWIAG
                     DSGLIINTRNNGLNWEPQFTGITNNIIELFFLNGNIGYALAWVLDATPPNYYGTKIIT
                     TKNGGQNWSVTMFPDSNVFLNTVFFRDSLNGYLAGSGGKLYYTSDGGAEWLLAPIDSG
                     MDFGFPVNKISFYDENYGIGVGGAIDIAGVVWKSTNGGRSWRTIVVGPEPINDFLFLD
                     KNNAIGVGGDFEYGSSQINSYNMGANWLYEEFQVFGIANTISYRTPQEGWISLGIVDS
                     FLVTTNGGQNWDLTGTPQQSNIYDIAFSSSRNGWAVGNNGVILKYNSELVGIADDINS
                     VNPNNFILHQNYPNPFNPKTVINYELQGTGNAKLKVFDVLGNEVAELVNEKQNAGSYS
                     VEFDGSGFASGIYFYKLTAGEFSETKRMVLLK"
     gene            complement(26244..27044)
                     /locus_tag="PLX80_01645"
     CDS             complement(26244..27044)
                     /locus_tag="PLX80_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19529.1"
                     /translation="MNLKTISIIALLILLSIGSVSVKADEDFDKAILKSKKALTEAMN
                     ISDEAKLIKARGQFERILQLKQQPWLVNYYMALADYGLALVGMRTEDKDKTKKYTESG
                     IETINKSIDENPEFCDSFVLLESLNFNRWQYEQEKMEEIISATQYADTEAKKLDANNP
                     RYVLVTGISQFYMPEAFGGGVKIALPTLEKSEELFKTYKPKSELYPDWGQDMSYGYIA
                     LCLLKRDDEGDKAKAKEMIDEGLKINPDSGFLTGYVMKEYNAGTEDKK"
     gene            complement(27102..29354)
                     /locus_tag="PLX80_01650"
     CDS             complement(27102..29354)
                     /locus_tag="PLX80_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY19530.1"
                     /translation="MKLIFTLIYTIILLMSCGNSFSQEKDSVKTEETVKTEKTVETTL
                     ISGKVTGEKETILVGVNVVIEGTIDGATTDEKGFYEFETEKFGPVNLIVSYIDYTTKT
                     VPLDIFRGQPVVLNVRLSKEEIRTDEILVTASSFTSGNNNAVTLTPLEIVRIPGADAD
                     IYRAITTFPGSNQVNEGSRITVRGGNPNEVLTIIDMASLYNPFIFDDDFNTSSYSTIN
                     PWGLKGINFTSGGFSAKYGNVLSAVLDLKSYDMPQGSGMFAWLGLANASLSGVYLSDD
                     RKLGATFTAGKLFLEPYFAINGKHSEYNPIPQSNQLGGTISYNIGKTGNLKAYGNYSD
                     DKVGIKNAGPTYDGYFEGSSNNYFANLKYTSAPSVTTLLGIGGSFSFNENNSAYGVLN
                     TTVQQTYSKGRAELTIQASNKTDINTGVEYEYSGYNINGIVPQFQYNLALNAPSTDLD
                     TSANTGRIGAYVEGQIRLTSDLFIIPGIRSDYQSLSEKVSFDPRFSFGFLLAKNQTVR
                     GAVGIYHQYPGLSEYYSSDDNNLEPEEAIHYILGYEFNKEGNIVFRVEGYYKDYSNLV
                     LRNVTDFFYGSEGKGFAKGVDMFFKTKISNKFTGWISYAYSDSKREQYDAAYIAPSNF
                     DITNNLSVVGSYNLTDKIVLGMSYKLSTGRPYTPVTGSNFNSNMNVYVPVYGDVNSGR
                     FPTYNRLDMNAQYIFSMFGKFAVGVLAVNNIFNQNNLYDYTYNTDYSTQVPIYTNNKR
                     SLYLGLGLQL"
     gene            complement(29361..29645)
                     /locus_tag="PLX80_01655"
     CDS             complement(29361..29645)
                     /locus_tag="PLX80_01655"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQY19531.1"
                     /translation="MEKLTQDQIEKNLKELTGWESTGSSIKKKFKTDGFPQTMGLVTA
                     IGAFCQQHDHHPDYMTMKYSEVEVSFSTHSAKGITQKDIDIAKDIEKLKF"
     gene            complement(29671..29973)
                     /locus_tag="PLX80_01660"
     CDS             complement(29671..29973)
                     /locus_tag="PLX80_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY19532.1"
                     /translation="MNYDYLQIDDVIHSRIRTSIMAVLVGIDEAEFNFLKEKVNATDG
                     NLSVHLKKLEQAGYISVKKLFVDRKPLSKYKITSKGYKAFENYLSNLENIIRSNKK"
     gene            complement(29973..30575)
                     /locus_tag="PLX80_01665"
     CDS             complement(29973..30575)
                     /locus_tag="PLX80_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19533.1"
                     /translation="MESRQAELELSLIKKMMEDTRKMNIDNGIHYIFWGVLVAVALFI
                     NYLFLKLNFYPKYVGLVWMVLMVSGAVADIFIAKYQSKRSSVSTYAGRLMSSLWLASG
                     VAMFIYGFIGPLSGAYNSIFICPIIATSLGISYFTSGSIQQLGWLRSVSFGWWAGAIV
                     MFIFPGIHTLLVFAVMIILLQIVPGILLNIKSKKFLERTA"
     gene            complement(30730..32169)
                     /locus_tag="PLX80_01670"
     CDS             complement(30730..32169)
                     /locus_tag="PLX80_01670"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQY19534.1"
                     /translation="MSKNTPITVAYGDGIGPEIMEATLNIITSAGADIDIETIEIGEQ
                     VYLRGNLAGIEPTAWDSLRRTKVFLKAPITTPQGGGYKSLNVTTRKTLGLYANIRPCI
                     SYHPYVSTKHPVMDVVIVRENEEDLYAGIEYRQTQEVYQCLKLISRPGSEKIIRYAFE
                     YAVANNRKKVTCFVKDNIMKLTDGLFHQVFREISADYPDIENEVWIVDIGAAKMANTP
                     EDFDVIVMENLYGDILSDVAAQITGSVGLAGSANIGQNFAMFEAIHGSAPRRAGQNLA
                     NPSGLLLGAVMMLNHIGQSETASKVHNAWLKTLEDGVHTYDIFKENISKEKVGTKEFA
                     KAVIERLGEVPTTLKSVEYKRSPTKKEITGVIAKVKIQQKDLVGVDIYLDWRNGSAED
                     LGEILKKIGTDVLYLAYISNRGVQVWPNGEPETFCTDHWRCRYHTVNKGDVINNSDVI
                     KLLEKVYDSGFDFIKTEHMYNFDGEVGYS"
     gene            complement(32255..33616)
                     /locus_tag="PLX80_01675"
     CDS             complement(32255..33616)
                     /locus_tag="PLX80_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HQY19535.1"
                     /translation="MSEINSNKYPKGIPYIIGNELAERFSYYGMKTILVVFMTQYLMN
                     SEGLDVMNDNEATKWYHVFGMATYFFPIVGAIISDVLWGKYKTIILLSVVYVLGHAAL
                     AIDESRIGLAIGLTLIAVGSGGIKPCVSAHVGDQFEKKNKHLLEKIFGYFYFSINLGA
                     AISTLLTPLLLEWYGPHIAFGVPGLLMLLATLVFYMGRKVFISIPPVGWKAYKLDLFS
                     ERGKKALVNLSVMYIFIAVFWSLFDQTGSSWVLQAEEMDKFVNLGFVKFELLASQIQA
                     INPLLIMIFIPMFTYFVYPMVNKIFPLTMMRKISIGMFMAAFSFALIAIAETSITAGN
                     TPSILWQFWAYVILTAAEVMVSITGLEFSYTQAPNSMKSIVMGLWFLGVSLGNGIAAL
                     VNEIIVNDDGSYKLEGSEYFWFFAILMMVTAFAFVLLAKRYKVETYIQERDISTNPLV
                     TEN"
     gene            complement(33669..34703)
                     /locus_tag="PLX80_01680"
     CDS             complement(33669..34703)
                     /locus_tag="PLX80_01680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY19536.1"
                     /translation="MKLDKSKIKKILVIKFGGMGDILLTTPVLPNLKAYFPDSEIYFL
                     TVRHSRDILIDNPYITRAFTYDASEDKSWCLIKNIRQQKYDLVIDLFGNPRTALITFM
                     SGAKYRFGFNFRGRNYAYNLKARGRGGEVHNVEFNLDSLRALEIPIISKKLYLPINVV
                     HEEFADEFFKANDLYSKKVIGISKTGGWESKRYKIDDYIKLMDMLNEIYDVNFVLFWG
                     NEKEKENCEKIKNSVKKQNAFLIPDSPIRYLAAIIKKCDIVIGNDSGPLHVAVSMEVP
                     TLGIYGPTNPMLQGPFGENNLSVVNENIDCLYCNLLNCNIGNICMTELSKDTIIDKVK
                     ELVRINNVNL"
     gene            complement(34700..35401)
                     /locus_tag="PLX80_01685"
     CDS             complement(34700..35401)
                     /locus_tag="PLX80_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQY19537.1"
                     /translation="MNSYFGNPFDVTKDRAGILDVLIATKLSQNTTDKSSFKAFTNLK
                     EKYGSWEKISEVNESSIRDAIKVCGLADTKSGEIKSMLNNLKKKFGSLDLKFMYEYSD
                     EEVYGEFLSHKGFGVKTISCVLIFAMGRDAFPVDTHVHRILNRLGIVKTSSAVKTFEK
                     AKDIIPIGKKYELHSNLIKFGRNICKAKNPLCSLCFLYDMCEFSEKGIYFKRNLSHTD
                     IKENNFIILENLRTS"
     gene            complement(35449..36576)
                     /gene="wecB"
                     /locus_tag="PLX80_01690"
     CDS             complement(35449..36576)
                     /gene="wecB"
                     /locus_tag="PLX80_01690"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQY19538.1"
                     /translation="MNKIISVVGARPNFMKMAPIHKQLLKHRSKVTHKIVHTGQHYDK
                     KMSDIFIEELDLPKPHIHLSIGSRSHAELVAMIMMEFEKIILKEKPDLVLVYGDINST
                     AAAVMVCSKILTKSGRTIPIAHIESGLRSFDRTMPEEINRMVTDVLADYLFVTEPSGV
                     KNLKKEGISSNKIFFVGNTMIDSLEFYLKKSKLSNVLKELCISEKKYTLVTLHRPSNV
                     DSKENLNKIISIFKNINKIDPSLDIVFPVHPRTIKMLEKFGLKDIFYSIKNLIVTEPF
                     GYLDFLNLIQNAKFILTDSGGIQEETTFLKVPCITLRENTERPVTSEIGTNVICGLDE
                     KKIIKHIKEIESGKFKKGKIPKLWDGKAAERIVKVLLKKIK"
     gene            36814..38082
                     /locus_tag="PLX80_01695"
     CDS             36814..38082
                     /locus_tag="PLX80_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19539.1"
                     /translation="MKILFFFLSMLCCTQIFSQGVWIHAGNISQSNNDNVYSMTSNSS
                     GDLFATSWAVGVFKSTDQGMSWSLSGLQGKRVSTLRTAPDGNIYALSKTVDFSYIHRS
                     TDNGNTWKDVDTRSFPLNYAGGGEIVFPSDGSIVASYSVTVGPLIGNVSTFVFKSTDN
                     GNSWVQTQRINAGFIGGMAITYDNNIFLGTSLGGVVYSSNNGNSFNLLNTFPQVFIAA
                     IFKAPDNSVYVSDAFNLHRSTDNGLSFTNAGINITNNYLGEAGVNSNGDLYVSMDDGN
                     VYLSENNGLNWSVINNGLPSSVNIKTFFSYLGKMYCGTGNSGIYYFDNLTSVNPNIEN
                     VNEFELYQNYPNPFNPVTNLKFNISAKGFTSLKIYNNIGREVAVIVKSELERGTYNYN
                     FDASNLPSGIYFYKLISGESSIVKKMNLIK"
BASE COUNT        12449 a         6578 c         6498 g        12701 t
ORIGIN      
        1 tggagatact gaccctgaaa atgtgatact gcttgagatc gaacctgaaa agcagaagac
       61 atatatagat ttcttatgca cggagaaata cataggagta aaatacgtct gcctgacaaa
      121 agtaatcaag gaaggcaaaa aactttatta tgaaaataac ggcaggaaaa taccaattga
      181 taaaatttac aacagggtaa tttacgatga gctttcgaaa agaaaggaca ttaaatataa
      241 tttcagattc accgaagagc tagatgtgaa atggatagcg catccaaact ggttcttcaa
      301 agtcagtaaa tattcactac cctttctcaa aagcaaatat gtccccgaga caaaatttct
      361 gagtgactat tcagaatttc cagccgacac agaaaactat attctcaaac ctctttactc
      421 ttttgcaggc gcaggagtta tctttgatct tacaaaagaa aatcttgatg caatccctga
      481 agaagacaga tcaaatttca tactacaaaa aaaaattaat tacgaacctt taatagaaac
      541 ttttgacgat ccggcaaaag cggagatcag acttctctat gtctggaatg acaaacctat
      601 tttaataaac aatctcgtcc gcctcagcaa aggtaagatg atgggcgtcg acttcaataa
      661 ggaaaagaca tgggtcggat cgagtattgc gtattttagg aagaagtaaa ttatttattc
      721 taataattat aaaccaatca agtttagatt atttattaca agaattctat catcctgcat
      781 tttactaaat taatatacac ttccatttgt aagctatttt tagaatcaaa aacttatatg
      841 aatttttatg tttttttaaa attaaattta tataaaattg taaatgtctg aaaaggaaga
      901 acagacttgt aaattttact gaatacaaat cttaagttga tatgtattga aaacatactc
      961 cgaaatccaa aagctaatat cttcttttta aaaagacatc atatcagata tccgtttaat
     1021 tgaaaaatgc atcctgttag aaaacatata agattgcgaa attatgatta ttcatcggaa
     1081 ggatattact tcatcacaat ttgtacagat gaaagaaaat gtttttttgg atatattgaa
     1141 aatggcataa tgattctgaa tgatattggg aaaatcgcaa atgaatattt attggaaatt
     1201 ccaaatcatt ttaaacatgc cttattggat gaatttacta taatgccaaa tcatatccat
     1261 ttgattttgg gattgaatta taatattccc tccgtacgga cacgccatgg cgtgtcccaa
     1321 caagatggta tatcccaaca agatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnncgaa tcaaaagaag atgacgtacg gacacgccat ggcgtgtccc aacaggatgg
     1501 cgtatcccaa caagatggcg tatcccaaca agatggtata tcccaacaag atatcgaatc
     1561 aaaagaagat gacgtacgga cacgccatgg cgtgtcccaa caggatggca tatcccaaca
     1621 agaacagagc acaaataaat ttggtaaatt aaacaaaaat tctgtttcag tgattattaa
     1681 tcaatttaaa tcatcggtaa aacgatggtg taataaaaac ggtcatgaaa atttcaagtg
     1741 gcagccgcgg tttcacgacc atataatccg aaatgaaggt gaacataaaa gaataaaaca
     1801 atatatcatt gacaatcctt caaattggaa ggaagataaa ctttctgata aataaattaa
     1861 ttatctaaca aatgaaactc gttggattac cgcatggaat aaatcactcc ttctttgtgt
     1921 ataaaaacac aaacaatttc cgaatccaaa aagtcagatt aatctaatta ctcatttaaa
     1981 aacccattgg cacaaccaac ttaaaattaa aattccttcc catattataa actcctttcc
     2041 tgccagtgac ttcatttaca tctccgtatt ttaatctgct gagatgactc tggtatgcta
     2101 tgtctgtcag attatttacg ctcattaata ccgagaataa attattgtta tttatgaaaa
     2161 cgtccgcgcc aaaactcata ttaaaaagtg tataacccgg agttgcagtc tcagtcccga
     2221 atgccgcaaa gaatttattc tgcgcaaaat aattttccat accaatctga aaatatgaat
     2281 tctgaaaatt gcatgtgaaa ttatttatgt cagcacgcag tacagtagta attcttgccg
     2341 gcggtgtctg aggcagatat tttgttgagt caggctggtt tgtctgaaca gactgcacat
     2401 aagaaaatgt attttcaaaa tgaagccagt caaatggatc aggatgaata tctaatgtaa
     2461 attctccacc gaataaatga gcatttcccg aagcatattt gtatgtgctg tatccgtctg
     2521 taatcgaatc acttcctgat atactttgca gcttttctaa ataaatataa ttgtcgatga
     2581 cattatagaa caggtctgct tctgctgcag cattcagtgt gttaatacca aatgtataat
     2641 caagctgaag gctattctcg gacttcagat caggtacacc gatgatataa ttaaatgtac
     2701 cttcatgaac tccgtttgat gacagctctg caatattcgg accgcgaaag cctttttgca
     2761 gatttatttt ggaataaaaa ttttcattga actgataaac agcaccaata cttcctgata
     2821 ttccactata attattatta aaatctgaaa acaaaggaat tgaattcgca tcatccggac
     2881 cggcaataac tcctgcagaa ttagtataaa gatcttttcc ctgcatagat ctcagatcga
     2941 accgcagtcc accgctgata tttaattttg aaaatgattt atttactatt cccaaaaatc
     3001 ctatatcgga taaattataa tcaggaataa gatattcagt acctttattt tttgatgtct
     3061 gatacataac atttgtgccg aaagaaaaat tgtatttatt tacttcaggt aaattatatc
     3121 tcgcttcgag gttataagaa tttaataaaa agtaaagacc atattgattc tgttcaaata
     3181 catctccaaa ttcctgacgc tgattttgtt gccatcctaa aactgtactc aagcttgcat
     3241 taccgagaat aaaattattg ttgagcgtga cttcataatg ataagttttt tgaaacggag
     3301 tattcagatc atatgaagta agctcttcat tatttacaac ttcagtacct tcggttgtat
     3361 cattttcggc aacggatttt acaaacatcc ctgtcacact gtctctgtct ccttctacaa
     3421 taccaggagt cagactataa gtactgaaat acaaatatga atatccccat gatttattaa
     3481 gaccaagcat tccactcaga ccgttttctt taaaacccga attgaaaaca tatccgtcat
     3541 atttattctg atatgcacgg gcgagtttat tgctgtatcg gatatcccag atgaaccctt
     3601 ttttatttcc agctatattc ccggtaattc caaacagtcc gttatttgtc tgatagtttg
     3661 aaaggagact tcctgaaatt tcaccttcgg gtaatgctgg agctgagatt aaatttataa
     3721 cacctgccat tgcatcagat ccgaatgaca gactagccgg tcctttgaga atttctacac
     3781 tgttgacaga atactcatcg atctccactc catgttcatc gccccactgc tgctcttcct
     3841 gccggattcc gttctttaca accaataccc tgttaaatcc taatccgcgg atgacaggtt
     3901 tggaaattcc tgaaccggtt gtgatctgtg aaattcccgg attaatactc aacgcatcta
     3961 taatatttgt tgctgatgac tgtattaact gagctggtga aacagtaatt accggtgtag
     4021 ggatttttaa aagttcggaa gcttttgata atcctgttac aattatttca ttaatttcta
     4081 ctgcggattc ttccatctca aaattcttta ctgaaaccac cgatagatcg atctcttccg
     4141 aaattagttt ataaccgatc atgcttaccc ggataagtac ttttgactgc ggtaattcat
     4201 tgatcttata tattccgtct gatcctgaaa atgtacctgt tctgagatca ggaaaattta
     4261 ttgaaacacc gggcataggt tctcccgttt ttttatctga tatttttcct gataagcctg
     4321 tgtttggaat ttttggagtg aatgtatttt tttccgctga agagaatttc ggaaacattg
     4381 caaacaattc aaaaactaca aataacaata ttatttgatt tgtcattttt acaatttaaa
     4441 tttgatttaa atgaagatga actattgatg tttattcaac tgaataaatt caacagtact
     4501 aaagtgtagc cggtattttg tgatcaggaa aaaaggggag gagctcttaa ggaaaaattg
     4561 taattttgga aatgttcaga taagaattgg cagacatgaa cagtaaccgg tttataattt
     4621 aagtctttgt taaaaagaat ttttataaaa taaatctcag ctgaagactt tgaaaattca
     4681 cagatcttgc attcatgttc agtaggatca aagtgaactg tattattgtc gtagcagtga
     4741 gtttcatcgc ggtgttcaag ggtgtgtgct gactcagcgg caaatggaaa gacgaagatc
     4801 agcaataaaa ttagagcaaa atatttttta aagctcgagt tgatagaggt gatttttatt
     4861 ttaaaaaagc agtaaacatt tttaaaaaaa ttactttaaa agtaacctgt ataaaataga
     4921 taatataatt ttcagattca attttttact tattctttta ttaaaaaagg gatattaaat
     4981 taagtccgaa cagaatgcca ggagttattg cactttttat ttaatgtgtc ggggataatt
     5041 tgattttgat aagaagttga aattataata ttttaattat taaaaatatc agtttctctc
     5101 aaagatacaa tcacaatatt gtattaaaaa attaaattta tgaacggagt tattcatcac
     5161 atagaaattt atgtttcaga tcttaataag tcaacggctt tctggcaatg gcttcttact
     5221 gaaaagtttt cttacaaagt atttcaaaaa tgggacagcg gaatcagttt taaatttgaa
     5281 agcagctata tagtatttgt gcagacggaa aagaaatatg cagccaattc atataacaga
     5341 aaaaatactg gactcaatca tctggctttt cattgtactt caagaaactt tgttgatgat
     5401 ttgacaatag atttgaaata cagaaacata gaaattttat atccagaaaa gcatccttat
     5461 gccggaggag aaaattatta cgcagtattt tttgaagatc ccgacagaat aaaagttgaa
     5521 gttgttgtct ctgaaaattg aaatttttct cctttcagtt ttatgaatac tgttaagatt
     5581 tcaaaaatat tattcaattt cttattagct tatataattc tattttataa aacaattttt
     5641 ttataacaga actgttctgc gtgatcaaag ctgtctttcg aaattcacag tgatggcaaa
     5701 ctagttcaga ccgactatct gaattttcaa aactgcagat aaattacttt taaaatacaa
     5761 atgaataata gacacattac tttttttttg ttgttagtca gtattatttt tatttacgga
     5821 tggggagcta acggtcacaa gatcattaac agaagatcga cattctcatt tccatcacca
     5881 ttgactttcc ttaactgggc tgacctcctt gctgctcatt cttcggacgc agactataga
     5941 agatcaagtg atcctaatga agcaccgaga cattttattg atattgataa ttacccggaa
     6001 tttattgcaa ctggcagaat tccgcaggat ctggattctc ttatcgcaat tcatggaagt
     6061 acatttgtat atgataatgg tattttacca ttttcaatta tggcaactgc agattcaata
     6121 aaaaaatatt ttcaattaca aaattatcca aaagcaatgc ttcatgccgc tgacttagga
     6181 cattatgtag gtgacggaca taatcctctg catataacaa gaaattataa cggacaatat
     6241 acaaatcaga atggagttca ttcaagatat gaaacgcagc taataaatcg tgactctgga
     6301 aacattattt attcatttga aagtgctgtt tacattccaa acattaatca gttcgtattt
     6361 gattttctgt ataataatta cacgtatgtg gattctgtac tgaagtatga cagcatagca
     6421 aatgcaatgg caccgggcta tggtacagct tattatcaga gatactggca gcttgcggga
     6481 aatttcacaa taatgttatt caataatgca tcgcacttta tttcaagtct tatctatact
     6541 tcgtgggtga atgccggcgg tgcagtgtca atttcagcaa cagaaacttc acttcctgaa
     6601 aaacctttcc tcgcacagaa ttatccaaat ccatttaatc cgtcgactaa aataaaattc
     6661 gggatcccgg atttacatca ttataatagc ggtggatctc atacgaaact aacgatttat
     6721 gatatttcag gaaaagagat tacaaatctc attaaccaac aacttcagcc cgggacatat
     6781 gaagtggtgt ggaatgccgc agaccatcca tccggaacat atttttataa gctttcagca
     6841 ggtgattatt ctgattcgaa gcgtatgttt ttattgaaat aatctggatc tttttgttta
     6901 gattttccca atcaccggat tagataaagt attcttaatt aataataatt tcactattta
     6961 aagatttttc agattataat tttcattttt gaaaagtttt ggaagcaatt ataaatttaa
     7021 cattaaaaat taattttatg aattgtaaaa aagtatgtaa ataattaaac tttacgaaat
     7081 ttatattttc aactcaagcc cccaaacttg gttattcaaa aaaagaaata tgaaacacaa
     7141 tcacactaat atatcaccgg atattaataa atacttatat attaaaccga ataaaaaatg
     7201 aaaactttta ctcttaaatt tgttttaaca attctgtttt tatctctgat acaaagcatt
     7261 tcatttgcac agccttacaa gatcaatctt tacaattcat atgtttttga tgatgggatt
     7321 gaatttgtca ctgataataa cagatatatc aatggtacaa tgaatggtgg atatcaatgg
     7381 ggaattggtt ttgaatatat tctgcaaaat tctgtaggga ttgaattaat gtataacagg
     7441 cagaatacaa attttccgat cacatacagt tcgactatag atactacaag atcctttgat
     7501 gtggatgtta attatattct gctcggttca aacaaatata ttccattgca aaataaaatg
     7561 atcaagccat ttggcggagt agccatcggt cttgcaatat tgaataatca gaatcctctt
     7621 ccgggagggg aatcttcatc tactaaattt gcctggtcag caagacttgg ttctgatttt
     7681 atgttttcac caagtgtcgg gatcaggata cagggtcagg ttcagtctgc agttcaggat
     7741 gtcggcggcg gtctgtatct tggaacggga ggtgtcagca caggcgtaaa tacagaatcc
     7801 tctatgtatc agttcggact gggtggatca ttaataattc agttcggcgc accgaagaca
     7861 aatagaagca gataatttca aaaaataaaa ctaaaaatat catgttgcat acaatgctaa
     7921 aaaagtggtg ggtgattctg atacagggta tattactttt tatactcggt atttttattt
     7981 tcaataatcc cgctgaagta cttgcaggta tttcactctg gatcggaata ttgttaatgg
     8041 taacaggtgt gatcggatta ttcggctgga tcttttcatc tagcggagac agagatacct
     8101 gggtactgat atggagtttg ctgactgcag tatttggggt aatagtattg atgaatatgc
     8161 tcgcagcaat gaaagctgtt acatttattt ttggaatatg ggttttgatt acaggattta
     8221 atcttacttc gtcaggatgg tctatgaaaa atgagaatgg actgggatgg ctgcttttta
     8281 taattggatt tctttcgatc tttgcagggt tcatgatgat ctttaatatc agtgcaggag
     8341 ctgtcggagt tgcaacgatc ctcggaattc aggtgctgct tacaggcatt gcaatgatat
     8401 tactttcgtt tgtgaaaaag gcattgtcaa ataaagtgaa aggtaagata gaagaattaa
     8461 aatcgagtat ttaatttcat tttaattaat ccgcagaatt tcaagagcac gtgactctgc
     8521 ggagacagac acttgatatg atgactgata ctatgagcgg tgctttacat taatattaat
     8581 tactggagtc agggaatttc tcccgggctc tttcatattt tcataaatca atctcttcaa
     8641 aaatttaagg aatgaaatca ttatttccat ttttaaaata aaattttatt gagagataat
     8701 attgttaatt tcattcccaa tttataattc ctttcaaaat aaatattaat tgtattaaat
     8761 catctctgtt atttttgatt tttattttta tttcttcctg cggtcagctt aataaagatc
     8821 aggaaaaaag taagttcaaa aataaaaatc cggaacattc cgataaaaaa tcttccgtca
     8881 caaatcaaat ttcaaaagat aatcttatca ctataatcgc agcaggggac gtaatgcccg
     8941 gatcaaatta cccttccgga tattctctgc cgccggatga cggtaaatat ttatttgaca
     9001 gtgtgaaaaa ttttactggc tcagcggatg tgaatttctg taatctcgaa ggtacgttat
     9061 taaactcagg aggcaaacct aaagaatgta atacgcctca taattgtgtt agcttccgaa
     9121 tccctgagca ttatgcaggc tatctaaaag atgcaggatt tgatcttgta agcacagcaa
     9181 acaatcacag caatgatatg ggagaggctg gaaaaatttc gacgattaat acacttaaaa
     9241 attatgatat taattttgcc ggatataatg atcatcctac agttgtattt gagaaaaatg
     9301 gtgtgacatt tggatttgcg gcatttgctc ctaataaagg aacacaggat ctgaataata
     9361 tttcgaatgc tgtaaaagta attttagatc tgagaaaaaa atgtaaaatt ttaatcgtct
     9421 catttcatgg cggtgcggaa ggatattctg cgcagagagt ttcaggtgat aaggaaattt
     9481 ttctaggtga agacagaggc aatgtatatg aatttgcgca ttcagttatc gatgcgggtg
     9541 cagatattgt gattggtcac ggtccgcatg taccgcgagc tatggaattg tataatggaa
     9601 aattaatcgc atacagctta ggaaatttct gtacttacgg aaaattcagt ctgagcgggc
     9661 cgcagggtat tgctccattg ctgaaagtat atttggatga taaaggcgat tttgtaaaag
     9721 gaaatattta ttcttttaaa caggtaaggc gtggtatgcc tgttccggat gaagaattga
     9781 atgcggtgaa tcttataaaa tctctgactg aaaaagattt tccgggaggt gatcttttaa
     9841 tatcagatga aggggaaatt acagttcgta ggtcagaata gtaccgtagg tcagaataat
     9901 attctgacat actctgaact gatctttttg tattttataa aataataaaa agttattttt
     9961 gtatacacat tttatctgaa tgaaaaatat actggtaatt cccattctta ttattttctt
    10021 attttcagaa aaaaattatt ctcagtcata tgacagcgga gatggttttt attctggatt
    10081 tggaacttcc atatcaagtt actggggagg atattttggg gaggcatatc agatgagagt
    10141 tttgtcttcg tatgattatt atgatgatat taattattca aatgataatt atgatgatta
    10201 ttctatctgg tctccatata ctatcagttt tactttcgga aaacattttt cggataattt
    10261 atcagtagaa gcggaatcgg attttctatt tcatcttaac ggcagagtag atccgcaatt
    10321 tgtaactgct gaaattggtg accaatattt tcaggacaga aacagttact cgactctctt
    10381 tgcaattccg atttctttaa gtattaagct gagtatggaa gatgaaagcg gcggagcatt
    10441 cattaagata ggtcctgcct ggcagtatac atccgagcaa tacgacagga tccggaatta
    10501 ttattcatat gaatattata acagttattc ttatgatgtc tatctatata atgtgtcgaa
    10561 agatgaatgg ctttttggat ttaaaacagg aatgggattg aaatattata tgtcagattt
    10621 tacttatgca gttaccgaac ttgaatatgc ctattttaat attaacccta caaataaaac
    10681 agcactcgcg ctggaccgtg ctcctgaagc acagttgttt tctttatctg caaaagtttt
    10741 ttttgatttc tgatcaggat tttttaattc tttcaaaatc ttgagaataa tattctgacc
    10801 ttccaaaatt attttacaat aaaatctttt cccgaatttg aattaataag atactcctct
    10861 acaaactctg ctatccctcc cgaataatta gtcacatttg taaatccggc atccgtaagt
    10921 atctggctgc taaacggact tcgacggctg tgtgaacaat acactatcac ttcactgtct
    10981 ttgtatttca ttatctctcc gatattttcc tcaagtatat cactggaaag attcacagca
    11041 tttttaaaat gtttgtatcc atgcaaagat ctaggacctt ttcctttgcc gttaaattct
    11101 tctgcagatc ggacatctaa aagtataaac cctttttcat tttcaagttt tgaaagaaat
    11161 tccggagtgt caatgctttt gaaaatcact ttactctttt cgatcaaagg catattgcaa
    11221 tctgaacaaa taccctgtga gtggtgaatc tctttatcac atccgctacc gcatggatag
    11281 cagacatatt ctgttttttc ctgagaatga gatttaagag gtaagagtaa aatcgatgag
    11341 attaagaaaa cgaaagtaaa aaatgaagtt ttcatattta aaaaatatta attaaaaaaa
    11401 tattttaatg aatcaattat tttttaatac ttagaaaagt aagttgggtt tcaatcaata
    11461 aaattcatat tgttcccaaa atttctgata gtctgaatat ccttatttta aaagaaccat
    11521 tttttttgat tcaaccaatc tgtctccggc aaagagtgaa taaaagtaaa caccacccgg
    11581 cagctcataa ccgctttgat caaatgcatt gaatgtaact tcgtatgtgc ctgtagtcag
    11641 atttgaattt acaagagtcg atacttcctt accgttgatg tcatagacaa cgagtctgaa
    11701 tttatttata ccattgcttt cagaaggaat gtcaatctta attttagtag atgggttaaa
    11761 cggatttgga aaattctgac tcatataaaa tccatttgga atttctgtac tgatctgatt
    11821 aattccaatg gtagcaagat cgatcggagc catccatacc tgatagacac ctgttatgtc
    11881 gtcattccag acggggaaga gtttattgtt tgtgtttgtg attccgatgt tgtctccctg
    11941 attacctgct cctgcacctg atacactttt gggaagaaat ttgtgatcac ttacaatgta
    12001 atctgtccag ttatcaccgc cgttagtcga acgtgaaaga tagatctgtg ctgagtcagg
    12061 tgaatttctg ttatccatat agatcacatt tattccgccg gattcatcaa tagtaagagc
    12121 agggaatacc tgattctttc cattattaag aggatcctga tttacacgga taccggaaga
    12181 ccaggtaatt ccgccgtcag aagatctgtg aaatacaaca tccggatcag atcccgccgg
    12241 tgaaaagttt acctgagacg aaacaatata aagccagccg tttctgctgc caccggtttt
    12301 gtcaatctcc aaagcgggaa aaccatttac acgaatattc cacggctgaa gagaagatga
    12361 cttaactccg ttgcatgcat aaatattttc attcacactc catgaatcac cgccgttagt
    12421 agaagttgca aaacccatat ttttttcatt aaaaggagag ctcaataatg aagaagccca
    12481 gacgacataa ttttttccgt cagatccgat taccatcgta ggtccgtttg attgtcgctg
    12541 cggaagtgag ttattgacct gagatactga agtccagttc agtccgctgt tgtttgaata
    12601 agaagtaaca atcggaaaag gatttgtgaa tcttgtccac acaagaaaag tcctgccgta
    12661 atacggactc atctgattat tatctgttgc aggatttcct ttgtcctggt cattacctgc
    12721 tatctgtaaa ttatttgtcc atgtctgtcc catatcggtg gatgtatttg aatacattcc
    12781 aagaataaat ccgccctgat gagttataat aaaatttccg tccttatcaa taatcggtcc
    12841 ggggtctccg ccgtgattag ataaaggctg acctgtgcaa gtgtcatttc cggtccatgt
    12901 gacaccgccg tctgtagtta cataaattcc ttcactctta aatgataatc ttattgtaaa
    12961 tgcgctagca aacattatat cgggatttaa tggatgtacg actaatgtgg gttctatctg
    13021 attattcagc gatggatgaa ttctgaaact tggatattgt gagtagatct cagagctgca
    13081 aaatattatt attaaaagga aaatgtaatt tctcatgtga aaaaatttag atgatttaag
    13141 agttaaaaaa tttagtaata aaattataaa ctttatatcc taaataaact tcatttagga
    13201 taatgatcat tgcagtaaac cgtaatacta aattgaataa ttcatataaa attatatgca
    13261 aataaaaaat tcctcaccgt taaaaaaaaa tgatcagatg aggaataaat taaaaaatta
    13321 aaaccggtta cctgtcgaat aaatatgaaa tattcagacc gccgaatata tttcctatcg
    13381 gaccggattc ataatattcc tgcctgtcag aatttatctg aacgaatgct acatattttt
    13441 tattgaaaat attattcatt cctaaatatg tcagaaatct gaattgtcca aaggtaaggt
    13501 cagcaccgaa ctgcactccc attagataat aagggtcagt tttcaaactg tcaacatttt
    13561 tatcacttac aaacattgaa cccacatatt gattggtatt ttttataaag acgttgtagt
    13621 tttcattaaa gtcataagtg taagaaagat ttgcattgaa ataattatcg ggatttgctg
    13681 gctcagtatt tcctgtgtaa tcttcttctg agatctgacc ggttcctgaa atacttcttg
    13741 ctgtatattc ctcatactta aaatttgaat atgtataact agtactgaaa tcaaatcctt
    13801 ttgcaatttg agtattcaaa ccaacttcta tacctgttct taatacctgt gcagcatttc
    13861 taaaaaaaac atttccatcc acagtaaaag gaataattgc attatcaatt tttgtagaat
    13921 acattgtcag ttccataaaa gtatttctga aaattttact gcctctgttt ggaatctcgc
    13981 ctttatatcc gagctcatag ttaacagtct gctgagggtt aaggtcagga ttgatcagct
    14041 tcaagccgcc gtcagatgaa aaaggataat tatctaattc attattagca gggacatcaa
    14101 atccaaatcc aaaagaacca tatatagaca tttgcggagt aagtagaaaa ttcagagcaa
    14161 actttggagt aaaattatca aacagtctgc ttgtatccct gaatccggca agcagatctg
    14221 ttgcagcagt gaccactctg tcatatcttc ccgtgatcag cagacttaat ttacccggta
    14281 aaacagggta ctgatttaaa aaataaaatc cgacattgga aagagtttca tcagtcaaag
    14341 caatcagatc atcacctttt acaccgccga tgttattgaa ttcgccgata ggaccagtct
    14401 gataaagcag atcacttccg acagaaaact cgttttccct gtttgcaata gtggatttat
    14461 taatgaatct gaatgtagct ccaagtccgt atctgttaaa aatcctgtaa gttgcatttg
    14521 tccttacaaa ttctttaatt gtaccatatc ctgtcacttc aataaaattt ttcttttctt
    14581 tatcaaagta tgaattataa gtaatgccga gtcttccttt atcagttaac ctctttgcat
    14641 ctctgctttc atcccttgca tttgcctgaa gatcatttgc attgtactga tcaagagtaa
    14701 gagatccggg aagtttgata agtccggaga cataataacc gtttataagg atcttttcat
    14761 tatcacttat atcagcttca taaattgaat tgaacctggt ctgatattca ttattgtgtg
    14821 ctctgtaccc attataattt tcataactgt aagtagccat aaatctttca cggtctgtct
    14881 tgacacctac tttgaatcca ttttttctga ggtcataaga tccgaattca ttatcggtaa
    14941 gtacaaaaga tgttggaaaa tatttatctg taaaaaagtt gatcacacca ccgggtgcat
    15001 ttgtatataa tgaactcagg tttccttttg caatttcgat cctgccgata gcagtaaagt
    15061 ccagtgcttc gagacgtgtt tgtccgtcag gctcagattc tggaattccg tcaagaagta
    15121 tcctaacgcc tctgactccg gtatttgatc tcgaaccaaa tcctctgatg gatactctta
    15181 catcatgatt cccgtatctt ggctgaagta taagcccggg tacagttgtt aacacgtcat
    15241 ttactcccat tttacgggat gatttccatg atgactgatc cactctctgt acggaaaaag
    15301 gaatgtcgat aagtctttgc tctgtcctcg tagcggaaat tattatctca tcagtttcgt
    15361 aatcggttgt atcttttgat tcctgagcca tgacagttcc cactgtaatg agaaaaaata
    15421 ctaaactttt ttttattaaa ttcttcatat gttttatttt ttttaataca tgaataattt
    15481 ttatatgaaa taaaaaatta tcagagataa ttaattatta tgaaaattca ctttgcagtt
    15541 ctgtgtcctt gtttctgtga aaattaaatt aataaaatac atcatcaatt atatcacatt
    15601 cattttctga tataaaatga ttatcaatca cattatgcct gttaatggat tttttaaaaa
    15661 tttcctgtgg aatgaatgtc tgtttggttt aagactggaa attaaaaaaa agtctttaag
    15721 ttctgcacct gttctgataa agatttatcc ttcggtgctg agttttaaag ttttgaaaaa
    15781 tctacctcta atttaataat ttaaatttgc agtggtaata ttatttgtta atatatgata
    15841 ttccaacatt agcattaaag aaagtaaatg aattatcatc tcctctgccg actatcggat
    15901 ttcctgcacc aagagcatag ttataatttg catttacatt aataccaaat ctttttccaa
    15961 tataaaaaat tatgccggct tcggggcctg ctccgaaatg caggttatta ttactcttaa
    16021 aatttgcacc aagctgtaat ctttcccata tataataagc accgacattt gcctgtatat
    16081 atggaaagac tcttaccata tatcctttat taaaaacgta accaatacta aaaagcatcg
    16141 gagtgtaatt cagatatctt gcctgcggac ctgtgataag accgttctga acttctgtag
    16201 ttccgtcgtc atatttattt gaaaggacat tatgacttat attaaatcca actgtaacac
    16261 tctgcgaaat gaattttttg attccgaatg aagcacccca gaaactcaca ttgctcacat
    16321 aatcatgagc tctgcctatg ttaaaagtag tgctgtattg aaaagtacct atataaaatc
    16381 ccttacctgt tttttgagca tttgcttcgg aacatataac tgaaactata aggaaaacaa
    16441 taattgaaat ctttttcaca aagtaaaatt ttatattaat taattaatta attttagaat
    16501 gatttttttg aaaaatcaca tctgattttt tagagatatg ttgactgcgt aaatgcttta
    16561 ttaatgctgt caatgatctt ctgtgagaca ttgcctcctg aactcataac tccgtttgat
    16621 aatccgatcc attgaattgg aagtgattca ccgttcagaa ctttaacatc tactatcaca
    16681 atgccgatag ttccggtgga atactcatat tcagtatcat aagaatagca actgctgtaa
    16741 ctccagacac caccccagca tccattattt tcagttacag tataagatgt tgttgtaacc
    16801 acattcccaa caattacaac tcccggatct cttgccattg aactatcagt gatccttgtc
    16861 cagccaagtg cattcagatt atttattgtc gtatttttaa ttgtattatc atatactctt
    16921 gttacatttg ctccattgat ctgaactaca gtatcaagaa agtaatattt agtaacatca
    16981 ttaaaattat aacccggatt aaaaaagctg gctacaatag gaatattgtt ggtagctaaa
    17041 ccatagtcaa cataacatga agaaatgact acaggggacg tcattaaaat aattaatgaa
    17101 aatattctta taaatcttaa agttctggac atttgatttt tcataggtta tgttggaaat
    17161 ttttaagaaa ctaaaaataa cttatataat aagtattatc aatcaagatt tttttcattt
    17221 taaataagtt ctttcaaaaa aaaagacaga ctgtaaacaa tctgcctttt ttatataaaa
    17281 attttggaga atttattgag tgtaagccag accgatattg gcattaataa aagaatacga
    17341 attattgtct gtatttccaa taggttcacc tgagctgaat gcataattgt atcttgcatc
    17401 aagaacgatt gcaacattct gaccaacatt aaatagcaca ccaacctgtg gaccgatacc
    17461 aaagtgccag ttatcattat caataatatt agcacctacc tgtaatcttt gctgtacata
    17521 atatgatccg atattagcct gtatatatgg gacagtttta cttttacctt tattaaagaa
    17581 atatccgaca gttgccataa caggtgcata attgataaat cttgcctgcg gacctgtaat
    17641 tgtaccattt tttacttctg tactgccgtt ttcctgctct tcagaaatta cattatttcc
    17701 gatcagaatt ccaaaagata tatttttttg tacaaaactc tttatttcca tagagccgcc
    17761 ccagaaacct ggagtactta aataatcaga agcttgtccc atattaaaag ttgcattata
    17821 ttctaaagat gcaagataag caccttttcc tgtcatattt tgagaataag agtctgaaaa
    17881 gatgaataca gttagaaaaa cggtaagagc aaataatttt attttattca ttatgttcaa
    17941 gtttatttta gtttttaaat tataaaaagc acattatctg aaatttcaaa tttacttcag
    18001 ataaggtgac tgagcaaatg cctgattaat actggtagta atccttgaag ttgtattacc
    18061 gccctgacca atcagtccgt ttccaacacc gatccattca attggaagat ttgaagcgct
    18121 tgaattacgc aaatctgaaa taactacacc gacacttccg gtagtataat cataagtatt
    18181 tccgtaatat ggatagcacc acgaatatcc ccagtaatca taccagcatc cgcttcctgt
    18241 attaacaacg tatgtggtag atgttacgaa tgttcctact acaactacac ctgatccggt
    18301 tgagtctgtg actcttctcc atccaagtgc atttagattt gctattgtag ttgatagaat
    18361 tgtattgtca taatctgttg taattgtacc gccgtctctg tgaattacag aatcgataag
    18421 atagtaatcg ttaacattat taaaattata atctggatta tagaaactgc caactacatc
    18481 ataattatct gtttctaaag tgtaatcagt ataacatgaa gaaaagatca caggacagat
    18541 aattattaat gcaaaaagta aatttctaaa tagttttaaa gaattttgaa ttttcatttt
    18601 tgtatatttt ttatttattt taatatggtg caaaataact tttatttaaa atatttacaa
    18661 ttcttaatat cagtttttta taaaacccgc ttatagtttg tatttttaaa aatctgttga
    18721 tgattttgaa taaaggatta tttaaaagta tttttgattt ttaagaactt tcaatacctg
    18781 atctgagtta ttcataactc agcttgttta tcttcttggc tttttattca gaacttaaat
    18841 aggtatttta accgattaaa ttttaaaatt aagaaaacca ttaaatgcaa aataagaaaa
    18901 attatttaaa actgttttca ctatttataa tgatctcttt tattgcagtt attgcatgtt
    18961 cctctaaaaa gaaacaggta gtagctgaag taggtgatga aaagatatat ctggatgact
    19021 atgaaacaca gtatatgaaa acggtaaata atattgattc agcaaagagt tcaaacatgg
    19081 aaaaacgcac cgaattttta gatctgttaa tcaaatacaa actaaaggta aaggatgcac
    19141 gtgaaagagg attattaact tctgaagata ttcaaaatga tctaaagcag tataaagaga
    19201 attttctgac ctcatttctg atcgataaag atatagtcga gccgatgata aaagatcttt
    19261 atgataaaaa agaatacgaa gtaagagctt cacacatttt aataaatctt ccaatgcaga
    19321 atatgtctcc ggaagattcc attgcagcat ataccaaagc atcagaagta cttgctaaat
    19381 tgaaagacgg agttgatttt tccgttgtag ctgctgatta ttctgatgac aattccgcaa
    19441 aacaaaacgg cggtgatctg tattatttta cagccggaat gacagtacct gaatttgaag
    19501 atgcacttta tgaattaaaa gttggtgaat atacaaaaga gccggtaaga actaatttcg
    19561 gacttcatat tgtaaaactc actgataaaa agaaaagaaa cgaaggaata aaggcatcac
    19621 acattctgat tcaggatcag aaagacagtc ttggaaatac aatcgattca atcggcacat
    19681 ataataaagc caaagaaact ctcagccgca ttaagaacgg cgaagatttt ggaaaggtag
    19741 cgacagaagt ttctcaggat cccggctctg caccaaaagg tggtgacctg ggattttttg
    19801 acagaagaag aatgattcaa ccttttgaca gtgttgcatt tatgttaaag gtagatgaag
    19861 tctctgacct tgtaaaaaca ccatatggtt ggcatatcat caaagtaaca gagatcaagc
    19921 cttatacttc tttcgaaaaa caaaaagaaa ctctaaaagg tgattacaaa aaaggtccgg
    19981 gatttaaagc tgaatataca aaatatattg ataagctgat aaaagaattc aaatatgagg
    20041 ttaataatga aggtctgaat tttttaatat caaaatttga cagcaataaa actttgtcaa
    20101 gctttaatat agattctatg tttaatgctg atgaaagagc aaaaataata gcaacataca
    20161 acggcggcga tgtaaaagta aatgattttg tagtatatat cacaaaaaac agagatgctc
    20221 aggcaagtct tgcaaacagt gagactttga gaaaagtaat aacaaacacc ggtgaaatgc
    20281 cgcttttata taaaaaagcg atcaaggaaa atatagaaga tgatccactt tatgtagaac
    20341 agctgactga atatgaaaac ggacttctga gctttaaagt agatcaggaa gaactctgga
    20401 gaaagatcaa aataaatccc gaggatcttg aaagttatta taacagtcat aaggataatt
    20461 attcttatac agatagtaat gaagtaaaag taaaaccttt tgaagaagta aaatcagaaa
    20521 tttcgaatac cattcagcag gataaattca aagagcttga aaaagaatat atcgaaaacc
    20581 taaaggcaaa gtatcccgtt aagataaacg ccgcaatttt aatcgaagca tttaaagaat
    20641 aaattaagat gttaaaagta atatttataa ttccactatt tctgattctg tcatcagcac
    20701 tttttgctca gcccgaaggt gacagaatta tagcagtagt aggaaaagaa attatcacag
    20761 aatcagattt ccagtatcag cttcagctat atgcgcgtca aaatcagctt gcagacattt
    20821 ctccttatat ggtacagcag attttccaat ctattctgac caataaaata attctggcaa
    20881 aagcagatca ggatagtatt tttatttctg aagaagagct gaacagggaa ctggagaacc
    20941 gtataaagaa tctgataggg caggtcggct cagcagaaaa acttgaggaa atgtacagca
    21001 tgtctatgac caagatcaag atccttttga aagaagatct tgaaaaaagt atgaagacgg
    21061 agaagcttaa aagacagaaa tttgcaggtg gtatgagggt ttcagatgca gaagttaaag
    21121 aattttatac ttcttatggt gatagtctgc cggatgtcag tgaagaactt gagatcgctg
    21181 caattattat ggaaagaaaa gtaagtgaat ctgagaaaaa agaagctgaa agaatagctt
    21241 tgcagatttt agacagtatt aaaaacggaa cggaattctc agatcttgcc agaagaaatt
    21301 ctgctgattc attatcagct attgaaggcg gtgatctcgg tatagcttcc aaaggtactt
    21361 ttgtaaagga atttgaagat gctgttttta gtctctctcc gggtgaagtc tccggtattg
    21421 tcgaaacaca atttggtctg catattatta aatccaatga aaaactcgga gataaagtaa
    21481 aagctcagca tattctcatc agatttccga aatttgaatc ttcggatttt gaaacactta
    21541 attttctgaa tgatcttcgt gataagatca ataaaggtga aattacattt gaagatgctg
    21601 ctaaacaata ttctgatgat ccgcaggcaa agagtaatgg cggttatgcc ggtaaggttc
    21661 cggctgaaca gtttgacagt gcgactgtag aaattttaaa taacctgacc gatggtcaga
    21721 taagtgagcc tatcagaatt ggaagcgata ccgaatatgg ctatcagatt attaaagtga
    21781 tcagtaaaat acctccgcac aaacttactt tgaaagatga ttatgaaaag gtaaaacgtt
    21841 atgctgaaaa ctataaggaa aataaagaga tggagtcatg gatcgaggaa ctgcgtgaaa
    21901 gtatttatgt agatattaaa atgtaatatc cggcttcatc aaaatcagct tgttttaaaa
    21961 aatatagttt taggaaggac aatgagactg aatttctcta gtcttttaaa tccaataatt
    22021 ccattaattg catttttcag caatttaaac aaaaggtttt gaatttgata agcttcctaa
    22081 aaccaacccg gtaaaatgaa tttttcattc agcgcagaat atggttttgt ctatgcagca
    22141 ataatgctga ttgtcgccgg tgcagtctca tatttttatt ataaaaaaac tatactcaaa
    22201 gaacctcaaa agaaattcct tactgctcta agagctttat cagttttttt tatttttatc
    22261 ttactgcttt ctccggttat gtcattcatt tcaaacattt ccccttcccc aaaaaatatt
    22321 tttctcatcg ataattctga aagtctttta attgaaaaca gaaataatct tttaaaaaag
    22381 atagtaactg atgaaattaa aggtataggt gcagggaatt cagaagatct ttattttctt
    22441 ttttcaggag atctgataaa ggaaatttcc gcagatgaac ttaacggaat tacttatgaa
    22501 ggtataaata attttgaaac agatctttct tcacctgtaa gcacacttca ggaaaaactt
    22561 tccgcagcaa atatttcaac tgtaaatatt gtctcagacg ggatgatcaa tgaaggtggg
    22621 aatccgttaa agtttgcaag gtcaatgaat gtaccattta attattatct gacaggtgat
    22681 acgattcaga aaaatgatgt atcaatcaaa aatctttact ataataagac agcttttgcg
    22741 gaaagtaccg tgcctgtaaa agttcagatc gcttcttaca attataatac aaatgtaaat
    22801 gtcagtctct atgaagagga taagctgatt gatacaaaga cagttgcggt aaatgaaaga
    22861 gataatttat atgatgtatc tttcaatgtg ttttctgctg aagataaaat tgtaaagtac
    22921 agaatagaat ccgagccgtt agaaaatgag atcactttaa aaaataattt cagagaattt
    22981 tttataaaat tcacagataa taaatttaaa gtactggtta tttcaggtgg tccgtcagcc
    23041 gataatgcct ttattactga agaacttaag caggtcagaa attttgaaac tactgtgctc
    23101 actcaaaaat caggcggaga attttatgaa ggcggatttc cggatattaa tatatttgat
    23161 acatttattt tgattggtta tccgactgca gtttcggatc agagtattct gaacaggatc
    23221 agtgctgaga tcgagaataa aaactcatcg cttatttttt ttgcaggaag gaatacggat
    23281 tacagtaagc ttatgtcaat tgaagacaaa ctgagtttta aaatttcttc atcatcagat
    23341 aatgaagagc tgacggggct ttcttcagta atttcaactg acaatgaaat tttcagcagt
    23401 cttgatcagc tcagatcagt cagtagtatg cctgacatat ttaaaacttc ttcaatcttt
    23461 tcatcaaatc catctgcaga tgtttttctt ttgacaacaa aaaattcaat tcctgcgctg
    23521 ataattgaaa acacaaagag taaaaagtca gcagcatttc ttgcttacgg tttttacaaa
    23581 tggagactga ataaaaataa taaaggtagt gatgatcttt ttagaaatat actgtcaaat
    23641 attattatcg caatcacaga caaggaagct caaaaacatt tttccatcga aacaacaaag
    23701 tccgtttatt caaaatatga agatgtgaaa tttaatgcat ccattctgaa tcatgaagta
    23761 aatggaaatg aaaagatact tgtaaaaatt tcaggaaacg gtatcgataa agaaactgat
    23821 ctgatcagat taaatgacaa tcaatattca ggaaagataa acattcctga agatggaaat
    23881 tatgaatata cagccggtct ttatgatgac aatgttttag tggaaaatat actgggaaga
    23941 ttttcggtag gggagaataa ttttgaattc tcagagacca gagctgacaa ttctgtattg
    24001 aaaatacttt ccttagaaac aggcggacag aatcttacag gaaaatccaa aagtgaaatc
    24061 gatgacattc ttacagaaat gaatggtaag tcggagtatg aattcaagac agcgcgtaat
    24121 ctggagctga acgtgaatcc gtattatctt gcactgttga tatttttatt gtgtcttgaa
    24181 tggtttttaa gaaagagaaa taatcttccg taatagccga aggtaactta tgttaaaaga
    24241 tagtaacatt atattttaga tgcgaaaaat attaaaaaga cggtttagct cctcctctct
    24301 gcccaaaggg gaggctggga agggtttgta aaaccaggtt tgatttgacc aaactattta
    24361 agggttgagt tttatgcaat atattattgc tgtttctttc ggcaacaacc ccttcgctcc
    24421 ccctttttag agggaaatac ttattaatta ttctaaaacg tgctgtttga aatttgattt
    24481 agattctaaa atattgagta aaaattttat ttcttttttg agaataattt aaattgttac
    24541 tggctaatgt actgtaagga aggatttaaa ttaagtgtta gtttaattaa tttaacatat
    24601 aaaattaaaa agtattaatt ccgtttgaaa aaatatttaa taatattgac tgtactttta
    24661 agttgtgctg ttgaagcagt ggctaatgat gattattggc ttatacagtc gaagcagact
    24721 tcacaaaatc tgtcatcatg tgaatttact gacacattaa acggctggat cgccggcgat
    24781 tcaggactca ttattaatac aagaaataat ggcttgaact gggagccaca gtttacgggc
    24841 attacaaata atataatcga attatttttt ctcaatggaa atatcggata tgctctcgcc
    24901 tgggtactcg atgcaactcc acctaattat tacggaacaa aaattatcac tacaaaaaac
    24961 ggcggtcaga actggagcgt tacgatgttt cctgattcaa atgtttttct gaatacagtt
    25021 ttttttcgtg acagtctgaa cggatatctt gccggatcgg gcggtaaact atattatact
    25081 tctgacggtg gtgctgaatg gcttttagca cctattgatt ccggaatgga ctttggattt
    25141 cctgttaata aaatttcttt ttatgatgaa aattatggta ttggagtagg aggagcaatt
    25201 gatatcgcag gagtagtatg gaaatccact aacggcggca ggtcgtggag gacaattgta
    25261 gtaggtcccg agccaataaa tgattttctg tttcttgata aaaataatgc gatcggagta
    25321 ggcggtgatt ttgaatacgg ttcgagtcag ataaattctt ataacatggg ggcaaactgg
    25381 ctgtatgagg aattccaggt gtttggaatt gcaaatacaa tttcttaccg aactccccag
    25441 gaaggctgga tatctctcgg catcgttgac agttttcttg tgacaactaa cggcggtcag
    25501 aactgggatc tgaccggtac tcctcagcaa agtaacatct atgacattgc attcagcagc
    25561 agcaggaatg gctgggcagt aggaaataac ggcgtgattc tgaaatataa ttctgaactg
    25621 gttggcattg cagatgatat taattcagta aatcccaata attttattct gcatcaaaat
    25681 tatccaaatc catttaatcc aaagacagtt attaattacg agttacaagg tactggtaat
    25741 gcgaagctaa aagtatttga tgtgctgggg aatgaagttg cggagcttgt caatgagaag
    25801 caaaatgctg gaagttattc agtagagttt gatggcagtg gatttgcgag tgggatatat
    25861 ttttataagc tgactgcagg agaatttagt gaaacaaaac gtatggtgct tctaaaataa
    25921 ttagaaatta aaaattagga attatgaatt tttattgcaa aaattttccc gcagagaatg
    25981 ggaatttccc taaaggattt ttattaatat atgtctttgc gaacccggat tcattcgggt
    26041 gaagcaattc gaattttata catatctata atttaaaaga ttactgattc caaaattgaa
    26101 tgattattca ccgctgataa tttttgtcgt agattcggaa attgctgtgc ttcgcccgta
    26161 aaatcatatc tttattgtaa aatgatcaaa aaaaaacgcc ggtgaaaaaa ttttccggcg
    26221 ttttaaattt tggttactat aaattacttt ttatcttcag tacctgcatt atactctttc
    26281 attacataac ctgttaaaaa tcctgaatcc ggatttatct ttaatccttc atctatcatt
    26341 tcttttgctt tggctttatc tccttcgtca tctctcttta aaagacagag agcaatataa
    26401 ccataagaca tatcctgacc ccagtccggg tacagttcgc tttttggttt atatgttttg
    26461 aaaagttctt cagatttttc aagtgtcgga agagcaatct tgacaccgcc gccaaaagct
    26521 tcaggcatat agaattgtga gatgcctgtg accagtacat atcttggatt atttgcatca
    26581 agcttttttg cctctgtgtc agcgtattgc gttgctgaaa taatttcctc catcttctcc
    26641 tgctcgtact gccatctgtt aaaattcagc gactcaagta atacaaatga atcacagaat
    26701 tcaggatttt catcaattga tttgttaatt gtctcaattc ccgactcagt atatttcttt
    26761 gtcttatctt tatcttcagt tctcatcccc actaaagcaa gtccatagtc tgctaaagcc
    26821 atgtaataat ttacaagcca cggctgctgc ttgagctgaa gtattctttc gaactgccct
    26881 cttgccttga ttagcttggc ttcgtcactg atattcattg cttcagttag agctttctta
    26941 cttttaagaa ttgctttgtc aaaatcttcg tcagctttta ctgataccga tccgattgaa
    27001 agcaaaatta acaaagcgat aattgaaatt gtttttaggt tcattttttt aatttctcct
    27061 tttgaaatta gtttttggtt tatgatttag ttagtaaaaa attataattg taatccgagt
    27121 ccgagataca gacttctttt attgtttgta taaattggta cctgtgtaga ataatcagta
    27181 ttgtaagtat aatcataaag attattctga ttaaaaatat tattcactgc taaaactccg
    27241 acagcgaatt ttccaaacat tgaaaaaata tactgagcat tcatatccag acggttgtaa
    27301 gttgggaatc ttccgctgtt cacgtcacca tagaccggta catatacatt catattactg
    27361 ttaaaattgc ttcctgtcac aggtgtatat ggtcttccgg ttgaaagctt ataagacatt
    27421 cccaatacta ttttatctgt gagattataa ctgccgacca cacttagatt atttgtaatg
    27481 tcaaaatttg aaggagcgat ataggcagcg tcatattgct ctctctttga atctgaatat
    27541 gcataagata tccagcctgt gaatttattt gagatctttg ttttaaaaaa catatccact
    27601 cctttagcaa atccttttcc ttctgaaccg taaaaaaaat ctgtgacatt tctcaataca
    27661 agattggaat agtctttata ataaccttcc actctgaaaa ctatatttcc ttctttattg
    27721 aattcatatc cgagaatgta atgtatagct tcttcaggtt cgagattatt gtcatcactg
    27781 ctgtaatact ctgacagacc cggatactga tgataaatac ctactgcacc tcttacagtt
    27841 tgattctttg caaggaggaa tccaaatgaa aatctcggat caaaacttac tttctctgaa
    27901 agtgactgat aatcagatct gatacccgga ataataaaca gatcactagt tagtctgatc
    27961 tgaccttcga catatgcacc gatcctgcct gtatttgcgc ttgtatcaag atctgtcgaa
    28021 ggagcattca gcgcgagatt atactgaaac tggggtacaa ttccgttaat gttgtaacca
    28081 ctatattcat actctacacc ggtatttata tcagttttat tgcttgcctg tatagtaagt
    28141 tctgcccgac cttttgaata agtttgttgt acagtcgtat ttaaaactcc gtaggcagaa
    28201 ttattttcat taaagctgaa agaacctccg atgccaagta gtgtagttac agaaggtgca
    28261 gatgtatatt tcagatttgc aaaataatta ttcgaacttc cttcaaaata accgtcgtat
    28321 gtcggtcctg cattttttat tccgacctta tcgtcactgt aatttccgta agctttcaga
    28381 tttcctgttt tgccaatgtt gtaagagatc gtaccaccga gctgatttga ctgcgggatc
    28441 ggattatatt cagagtgctt tccgttgatt gcgaaatacg gttcgagaaa taatttccct
    28501 gctgtaaaag tcgcgccgag ttttctgtca tctgaaagat agactccgga aagtgaagca
    28561 tttgcgagac caagccatgc aaacattccc gaaccctgag gcatgtcata agattttaga
    28621 tcaagtacag ctgacagtac gtttccatac tttgcagaaa atcctccgct cgtaaagtta
    28681 atccctttta atccccatgg attaatagtg gaataagacg acgtattaaa gtcatcatca
    28741 aaaatgaacg gattataaag tgatgccatg tcaatgatgg taagtacttc atttggattt
    28801 ccaccgcgta ctgtaatacg gcttccttcg tttacctgat tggatccggg gaatgtagtg
    28861 atggctctgt aaatatcagc atctgcgccg ggtattctta ctatctcaag cggagtcagt
    28921 gtgacggcat tgttgttacc tgatgtaaat gaacttgcag tgaccagtat ttcatcagtc
    28981 cttatctctt cttttgaaag tcttacattt aaaactacag gctgccctcg gaaaatatct
    29041 aaaggtactg tctttgtagt atagtctatg taagagacaa ttaaatttac cggtccgaat
    29101 ttttctgttt cgaattcata aaatcctttt tcatcagtgg tagcaccgtc tatagttcct
    29161 tcgatcacaa cgttgacgcc gacaagaatt gtttctttct ctcctgtgac tttacctgat
    29221 ataagtgtcg tctcaactgt cttttcagtc ttaacagttt cttcagtctt tacagaatct
    29281 ttttcctgtg aaaaggaatt tccgcaagac atcaacaaaa ttatagtata tattaatgta
    29341 aaaattaatt tcatgtttgt ttaaaatttt aatttttcaa tatcttttgc aatgtcgata
    29401 tccttttgag taattccttt tgcagaatga gttgaaaatg aaacttcgac ttcggaatat
    29461 ttcattgtca tataatccgg gtgatgatca tgctgctgac aaaatgcacc gatagcagtg
    29521 accagcccca tagtctgcgg aaatccgtct gttttaaatt tctttttaat tgatgatccg
    29581 gtagattccc atccggttag ttcctttaaa tttttttcga tctgatcttg tgtaagcttt
    29641 tccattttaa atatatttta agttagttag ttatttttta tttgatctga taatattttc
    29701 gagattactc aaatagtttt caaaagcttt atatcctttt gaagtgatct tatattttga
    29761 aagtggttta cggtcaacaa aaagtttctt tacgctgata tatcctgctt gttctaattt
    29821 ttttaaatgt acactcagat ttccgtcagt tgcatttact ttttctttga gaaaattaaa
    29881 ttcagcttca tctattccca caagtactgc cataatggaa gttctgatcc gggaatgtat
    29941 tacatcgtca atttgaagat agtcataatt cattatgcgg ttctttccag aaatttcttt
    30001 gatttaatgt tcaataatat tccgggtacg atctgaagaa ggattatcat aactgcaaat
    30061 acaagcaatg tatgaatccc cggaaatatg aacatgacaa ttgcacctgc ccaccatcca
    30121 aatgaaaccg atctcagcca gccaagctgc tgtatcgatc cgcttgtaaa atatgaaata
    30181 ccaagtgatg ttgctattat aggacaaata aaaatcgaat tgtatgcacc tgataaaggt
    30241 cctataaaac cataaataaa cattgcaact ccggatgcca gccataaaga tgacattaac
    30301 ctgccggcat atgtagagac actgcttctt ttgctttgat atttagcgat aaaaatatct
    30361 gcgaccgctc ccgataccat aagtaccatc cagacaagac ccacatactt aggataaaag
    30421 ttcagtttta aaaacagata atttataaat aatgcaactg ctacgagtac gccccagaag
    30481 atataatgaa taccattatc gatattcatc ttgcgtgtgt cctccatcat ttttttgatc
    30541 agggatagtt ccagttctgc ttgtcttgat tccatttttt atttaattaa agtgctttgt
    30601 aaaataaagt gctttacaaa attagcagat ttaaaattaa ttgtcaatat ctaaaatttt
    30661 tttattaaaa aaaaacttcc ggaatcaaat gaactgaatc cggaagtata atttataaaa
    30721 acattcagat caggagtatc ctacttctcc gtcaaagttg tacatgtgtt ctgtcttaat
    30781 aaaatcaaat cctgaatcat aaactttttc gagcagtttt atcacgtccg aattattgat
    30841 cacatcacct ttatttacag tgtgatatct gcatctccag tgatcagtgc agaatgtttc
    30901 aggttcacca tttggccaga cttgcacgcc tctgttgctg atgtatgcaa gatataaaac
    30961 atccgtgccg atttttttca atatctcacc gagatcttca gcacttccgt ttctccagtc
    31021 gagatatata tccactccga ccagatcttt ttgctgaatt tttacctttg ctattacacc
    31081 tgtgatttcc ttttttgtag gtgatctttt atactctact gatttaagag ttgtcggaac
    31141 ttctcctaat ctttctatta ctgcttttgc aaattccttt gtaccgactt tttctttgct
    31201 gatattttct ttgaaaatat cataagtatg tactccgtct tcgagagttt tcagccaggc
    31261 attatgaact ttacttgcag tttcactctg accaatatga ttcagcatca ttactgcgcc
    31321 aagcagcaga ccggatgggt ttgcgagatt ttgaccggct cttctcggag ctgatccgtg
    31381 aatggcttca aacattgcaa agttctgtcc aatgtttgct gatcctgcaa gaccaactga
    31441 tcctgtgatc tgagctgcga catctgaaag tatatcacca tataaatttt ccatcacgat
    31501 cacatcaaaa tcttcaggtg tgtttgccat ttttgctgca cctatatcaa cgatccatac
    31561 ttcattttct atatcgggat aatctgccga gatctctcta aagacctgat gaaataaacc
    31621 gtcggttaat ttcataatat tgtcttttac aaaacaagta accttctttc tgttatttgc
    31681 aactgcatat tcaaaagcgt acctgattat tttttctgag cccggtcttg agataagctt
    31741 aagacactga tagacttcct gagtctgtct gtattcaatt ccggcataaa gatcttcttc
    31801 attttctctg acaatgacaa catccatcac aggatgcttt gtggaaacat aaggatgata
    31861 agaaatacag ggtcttatat ttgcataaag cccgagagtc tttctggtag tgacattcag
    31921 acttttatat ccgccgccct gcggagttgt gatcggagct ttgagaaaaa cttttgttct
    31981 gcggagtgaa tcccaggcag tcggttctat accggctaaa ttccctctga gataaacctg
    32041 ttcaccgatc tcaatggttt ctatatcaat atctgctccc gccgaagtaa taatatttaa
    32101 tgttgcctcc attatttcag gaccgatacc atcgccgtat gcgacagtta taggtgtgtt
    32161 tttagacatg aaatcctttc tgttattttt ttagttttcc aatttaacga tatgtatcta
    32221 tattttaaat ctatttttat tgtaatttta aaactcagtt ttctgtaaca agcgggtttg
    32281 tagaaatatc tctttcctgt atgtaagtct ctactttata tcttttggct aacagtacaa
    32341 atgcaaatgc agtgaccatc ataagtattg caaagaacca aaaatattct gaaccttcaa
    32401 gtttataact tccgtcatca tttactataa tttcatttac tagtgctgct ataccattac
    32461 caagtgaaac gcccagaaac caaagtccca tgacaataga cttcatactg tttggagctt
    32521 gtgtataaga aaactccagt cctgtgattg agaccattac ttctgctgca gtcaagatca
    32581 catatgccca gaactgccat agaatgctcg gagtattgcc agctgttatt gaagtttctg
    32641 caattgctat cagcgcaaaa gaaaatgcag ccataaacat accgatcgaa atttttctca
    32701 tcatcgtcag aggaaatatt ttattaacca tcggatatac aaaatatgtg aacattggaa
    32761 tgaagatcat tatcagcagc ggattgatag cctgaatctg tgatgccagt aattcaaatt
    32821 tcacgaatcc gagatttacg aacttatcca tctcctctgc ctgaagtacc catgatgagc
    32881 cggtttgatc gaacagtgac cagaataccg ctataaaaat atacattaca cttagattca
    32941 ccagtgcttt tttacctctc tcactaaaca agtcaagctt atatgccttc caacctaccg
    33001 ggggaattga tataaatact ttccttccca tataaaaaac aagtgtagct aaaagcatca
    33061 gtaatccggg gactccgaat gcaatatgcg gaccgtacca ttctaataat aatggtgtca
    33121 gtaaagtaga aatagcagct ccgagattta tggagaaata aaaatatcca aatatttttt
    33181 cgagaagatg tttgttcttc ttttcaaact gatctccgac atgagcggag acacagggct
    33241 tgataccacc ggatccgact gcgatcagtg taagtccgat agctaaacct atcctcgatt
    33301 catcaatcgc aagtgccgca tgtccgagca catagaccac tgaaagcagt atgatagttt
    33361 tgtattttcc ccagagtacg tcagaaatta ttgcaccgac tatcggaaaa aaatatgtag
    33421 ccataccaaa tacatgatac cattttgttg cttcattatc attcatcaca tccagtcctt
    33481 ctgaattcat aagatactgt gtcatgaata caacaagtat ggttttcatc ccatagtaac
    33541 taaatctttc cgcaagttca ttgcctataa tgtacggtat accttttgga tatttattac
    33601 tgttaatttc tgacatataa ataattattt aataaaataa aaattatact tctaattaaa
    33661 aacttaattt acaaatttac attatttatt ctgaccagtt cttttacttt atctattatt
    33721 gtatcttttg aaagctctgt catacaaata tttccaatat tacaattaag taaattgcaa
    33781 tataaacaat ctatattctc atttacaacg gaaagattat tttctccgaa aggaccctgc
    33841 agcattggat ttgtcggacc gtaaattcca agtgtcggga cttccattga aaccgctaca
    33901 tgcagcggtc ccgagtcatt tccaattaca atgtcacatt tcttgattat agctgccaga
    33961 tatctgatcg gactgtccgg tataaggaat gcgttttgct tctttacaga atttttgatc
    34021 ttttcacagt tctctttttc tttttcattt ccccagaaaa gtacaaaatt tacatcataa
    34081 atttcattca gcatatccat cagtttaatg taatcatcaa ttttatatct cttcgattcc
    34141 catccgcctg tctttgatat cccgatcact tttttagaat aaagatcatt tgctttaaaa
    34201 aactcatcag caaattcttc atgcacaaca tttataggca ggtaaagttt ttttgatatg
    34261 attggaattt ccagtgctct tagtgaatcc agattgaatt caacattgtg tacttcaccg
    34321 cctctgccac gggctttcag attatacgca taatttcttc ctctgaaatt aaatccaaat
    34381 ctgtatttcg cgccgctcat aaatgtaata agtgcagtcc tcggatttcc aaataagtct
    34441 atcacaagat catatttttg ctgtcttata tttttaatca gacaccatga cttgtcttcg
    34501 cttgcatcat aagtgaatgc acgcgtaatg taaggattgt cgataagtat gtcacgtgaa
    34561 tgtctgacag tcaggaaata tatctctgag tccggaaaat atgctttaag attcggaagt
    34621 acaggagtag taagcagtat atcgcccatt ccgccgaatt taattacaag tatctttttt
    34681 attttagatt tatctaattt caactggtcc gtaaattttc tagtataata aaattatttt
    34741 ctttaatgtc agtgtgggat aaatttcttt taaaataaat tcccttttca gaaaattcac
    34801 acatgtcata caaaaaacat agactgcata aaggattttt tgctttgcag atattccttc
    34861 caaactttat aagattcgaa tgcagctcgt atttttttcc tatcggaata atgtcctttg
    34921 ccttctcaaa ggtttttact gccgaagatg tcttaacgat tccaagccgg ttcagtatcc
    34981 tgtgaacatg tgtatccaca ggaaatgcat ctctccccat tgcaaatatc agcacgcatg
    35041 aaatggtctt tactccaaaa cctttgtgag aaagaaattc cccatacact tcttcatcag
    35101 aatattcata cataaatttc agatcgagac ttccaaactt cttttttaaa ttattcagca
    35161 ttgatttaat ttctccggac tttgtatctg ccagtccgca aacctttatt gcatccctga
    35221 tagaactttc gttaacctca gaaatctttt cccagcttcc gtatttttcc ttcagattag
    35281 taaatgcttt gaaggaagat ttatcagtcg tattctgtga aagctttgtc gcaattaata
    35341 catccagtat tcctgcccgg tcttttgtta catcaaaagg atttccaaaa taactattca
    35401 aagcggaaat gactttacgt agttttttct taactgcagc ttccggattt attttatctt
    35461 ttttaataaa acctttacta tcctttcagc ggctttaccg tcccagagct tcggaatttt
    35521 tcctttttta aatttaccgc tttcgatttc tttaatatgc tttatgattt ttttctcatc
    35581 aagtccgcat atgacattgg ttccgatctc agatgttaca ggtctttctg tattttccct
    35641 taaagtaatg cacggcactt ttaaaaatgt agtctcctcc tgtattccgc ctgagtctgt
    35701 tagtatgaat tttgcatttt gtatcagatt cagaaaatcc agatatccaa aaggctctgt
    35761 aactattaaa tttttgatag aataaaaaat atctttgagt ccgaattttt cgagcatctt
    35821 tattgtcctt ggatgtacag gaaatacaat gtctagtgac ggatcaattt tgtttatatt
    35881 tttaaaaata gaaatgatct tattcagatt ttctttactg tccacattcg aaggtctgtg
    35941 aagagtgaca agtgtatatt ttttttctga aatgcaaagc tcttttaata cattagatag
    36001 tttagatttt ttaagataaa attcaagtga gtctatcatc gtatttccaa caaagaatat
    36061 tttattgctg cttatacctt ctttttttaa atttttaaca ccgcttggct ctgtaacaaa
    36121 aaggtagtct gccaaaacat ctgttaccat tctgtttatc tcttccggca ttgtcctgtc
    36181 aaaactcctg agtcctgatt ctatatgagc tattggaatt gttctgcctg atttagtcag
    36241 tattttcgaa cagaccatca cagcggcagc agtagaattt atatcaccgt acacaagtac
    36301 gagatcaggc ttctctttta aaataatttt ttcaaactcc atcatgatca tcgcaactag
    36361 ttctgcatgt gatcttgagc cgatacttag atgaatgtgc ggttttggaa gatcaagctc
    36421 ctcaataaaa atatcagaca tttttttgtc ataatgctgt ccggtatgta caattttatg
    36481 agtaactttc gatctgtgtt tgagaagctg tttatggatg ggtgccattt tcataaagtt
    36541 cggtctggct ccaactactg aaatgatttt attcaagaat tcaatttatt aaatatccgg
    36601 atttgatttt tatgaattta tgaaaaatat agattagttt aaattgaatt aaaggtttgt
    36661 atattttatg gttttaaaga ttttgaaatg gataatttca caagttgacc tcaaagttag
    36721 aatactacaa ttcagcatta tattgttttg tgaaattgta aaaatatatt taaattagtt
    36781 caacggattt taattaaaca atctttaatt tttatgaaaa tattattttt ctttttatcc
    36841 atgctatgct gcacacagat cttttcacag ggagtctgga tacatgcagg aaacatttct
    36901 cagagtaata atgacaatgt atattcaatg acatcaaatt cctccggcga tctttttgca
    36961 acaagctggg ctgtgggagt ttttaaatcc accgatcagg gaatgagctg gagtctatcg
    37021 gggcttcagg ggaaaagagt atctacactg agaaccgctc ctgacggaaa tatatatgct
    37081 ttatcaaaaa cagttgactt ttcatacatt cacagatcca cagacaatgg caacacatgg
    37141 aaagatgttg atacacgttc ctttccactt aattatgccg gaggtggtga aattgtattt
    37201 ccttcagacg ggtctatcgt agcatcatat tccgttacag tgggaccttt aattggaaat
    37261 gtatcaacct ttgtatttaa atctactgac aatggaaata gctgggttca gacccaaagg
    37321 ataaatgcag gatttatcgg tggtatggca attacatatg acaataatat tttcctcgga
    37381 acttcactgg gcggtgtagt ttattcttca aataacggga atagttttaa tttacttaac
    37441 acctttcctc aagtctttat agcagctatt ttcaaagcac ctgataattc tgtgtatgtc
    37501 tctgatgcat tcaaccttca cagatccaca gataacggac tttcttttac gaatgcagga
    37561 ataaatatca ctaacaatta tcttggtgaa gccggtgtaa acagtaacgg tgatctttat
    37621 gtctcaatgg atgatgggaa tgtttattta tctgaaaaca acgggttgaa ctggtcagtg
    37681 atcaataacg ggttaccttc ctcggtaaat ataaaaacat ttttttcata tttgggaaaa
    37741 atgtattgcg ggaccggcaa ttccggaatt tattattttg ataatttgac ttcggtgaat
    37801 cctaatatag aaaatgtaaa tgagtttgaa ctttaccaaa attatccgaa cccatttaat
    37861 cctgttacca atctgaaatt caatatttct gcaaaaggat ttacatcttt aaagatctat
    37921 aataatatcg gcagagaagt tgcagtaata gttaaatccg aacttgaacg cggaacatat
    37981 aattataatt ttgatgcttc aaatttacca agcggaattt atttttataa gcttataagt
    38041 ggcgagagta gtattgtcaa aaaaatgaat ctgataaaat aaaattcttc ggaacaaaac
    38101 tttcagcctt cagttttata atttgcaatt aactttaaaa cgggaggcta aaatgtttaa
    38161 cagaaaaagt aatgattatg acgtctttca ggaaattaca agaaagatga agtacggagc
    38221 acctgagtta aaaaagtttt acaatgtctt tttaataaaa ggattagctg ttgcagtaat
    38281 tactgcatcc ataattgctt tatcaagctt taaatatata agcagt
//
LOCUS       DAORVQ010000009        34936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_368_length_34936_cov_6.801411, whole genome shotgun sequence.
ACCESSION   DAORVQ010000009 DAORVQ010000000
VERSION     DAORVQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 34936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34936
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_368_length_34936_cov_6.801411"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..2166
                     /locus_tag="PLX80_01700"
     CDS             481..2166
                     /locus_tag="PLX80_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19540.1"
                     /translation="MKKVYQKFKDYNTRRNVLKYRLYKFLKKHSKVTEDSPVIIVWDM
                     GGFADLLKKNAMFAAALNLRGYKTHFIICDGTPEACIQRGLEKNEKVEDWDKKCVTCT
                     KAMEYYSREYSAEYSHAGDYISSEKKDEFLNLSNTIKIEDIFAYKFLELSVGSLAWSS
                     VIRYMKGYVIELKDLKKEDEIIYRKYFFAGLVNTFIANEVINKFKPVSLYSSHGVYVD
                     YSPPVLLAYIRGIKTLCWSSGFKDFLHYFTIPKDRNKLEFRGITEPEWKKRAAVSLTE
                     KENEFLYSYIFHRFNKGKGKDFLNVTLPETEDQLREKLGFEKGKKIVCLFCHVNWDLT
                     FDLSKMVFDNANQWLSQTLEAIFKNDKVNWIIRVHPGEKASGSLYTNDDFLKERYPDI
                     PSHVRILWSDSEINSLGLYKLLDIGITLFGTMGSELPLFGKPVISAGEAHFTGKGFSI
                     DTKTREEYFKCIETIQDIEPLNPDKIEIARKYAYSYFVQRQVPLGIIVKSEGHFGNLD
                     YKKLDHLLPGKDLIIDAICESIIEGKDVILDEKMLKVSEQDISLEIMPIQLNV"
     gene            2335..2718
                     /locus_tag="PLX80_01705"
     CDS             2335..2718
                     /locus_tag="PLX80_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19541.1"
                     /translation="MKNNFLFYSVCITILFLSSSVFSQSKIVTGTYKGEPLAYYKDKL
                     IVAYLTDSVNNIEYIHRVNDFIRKHNSSIDSVISILQYREIDITFYSNNADAVDLIDI
                     FKDSGLFEFVSLSGLIVPEMIKPED"
     gene            2929..3903
                     /locus_tag="PLX80_01710"
     CDS             2929..3903
                     /locus_tag="PLX80_01710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY19542.1"
                     /translation="MSKRISVIIPTYNRVGTLGITIKSLIEQTYPDNLYEIIIADNNS
                     SDNTKELVESIAKDSAVPVKYLFEKRPGAHFARNTAAQMSDSEYLYFTDDDMIADPDM
                     LENILKVFDLDYNIAVTGGKVLPKWEFDPPEWLLKYFQDGTLSLINKPEKLIITNHDF
                     GIYSCHQAMLRSVFFECGGFNPDIEKDKLIGNGETGLNIKILKKGYNFAYTADSVTRH
                     IIPRSRMTQKYLNNRFSNQGICDGFTNFRKSEYSKKNLFRLIFNSHLPLLLKSFSDSF
                     LNQIKGNSLWRLKRANFHYHYNRIKFDMKLISNDEWRKFVLRNEYFNY"
     gene            complement(3907..5112)
                     /locus_tag="PLX80_01715"
     CDS             complement(3907..5112)
                     /locus_tag="PLX80_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY19543.1"
                     /translation="MKKILTKNILLLSAVSFLTDISSEMLYPVMPIYLKSIGFSVLLI
                     GILEGIAEATAGLSKGYFGKQSDVTGKRTPFVTIGYLLSSLSKPMLGLFVYPVWIFLA
                     RTMDRLGKGIRTSARDAILSSETLPENKAKVFGFHRGADTLGAAIGPFLALIYLSIYP
                     EQYRTLFFIAFIPAILGVLFTFLIKEKEKDNSEIIKRPKASKYNFFGFLGYWKESVPE
                     YKKLVKGLFAFTLFNSSDIFLLLMIKHLGYSDEKVIWIYIFYNLVYALTSYPSGIIAD
                     KFGFRKNFIAGLLIFVFVYAGMALGPDQVFLYILFIAYGLYASFTEGVSKAWITNISQ
                     KKDTATALGFYTGLNSLFSLAASVIAGLIWYNFNPEAMFLFSASGTMLVVFYFLFGRI
                     KLSQNKMTV"
     gene            5286..5465
                     /locus_tag="PLX80_01720"
     CDS             5286..5465
                     /locus_tag="PLX80_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19544.1"
                     /translation="MKAPAKIFLALIFYFSLSTFLFQNSFAQLVSDFRVNDDSTNTSQ
                     YGAKTGVDGKENFLQ"
     gene            5989..7350
                     /locus_tag="PLX80_01725"
     CDS             5989..7350
                     /locus_tag="PLX80_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HQY19545.1"
                     /translation="MKDKIRIASGQGFWGDLVDAPYNQATGGPVDYIMMDYLAEVTMS
                     ILQKQKLRDPEFGYARDIPPLMERLLPVIKEKNIKVITNGGGVNPVACRDAIFKVAEK
                     IGVKGLKIGIVQGDNILDNIDDLVSKGIELKNMETGESVKTVRDKILSANVYLGAAPV
                     VECLKQGADIVITGRVTDTGLTLAPMIYEFGWNMNDWDLMASGTVAGHILECGGQASG
                     GNFLGDWKSIPDLANIGFPIAEAFPNGEVIITKHENTGGRVSIETVSEQLVYEIGDPK
                     DYITPDCIADFTSIQLEDVGENRVRVFGVKGLPATPFFKVSMSYSDGYSAVGSLTYSW
                     PDALDKAKAADEILRTRLSNLGLKFDEIRSEFQGYNACHGPLAEKFDDLNEVVLRFAV
                     RSQDKSALERFGKEIAPLILTGPPGVTGFAGGRPKPSDVVAYWPALIPKEEVESIAET
                     MSN"
     gene            7469..7909
                     /locus_tag="PLX80_01730"
     CDS             7469..7909
                     /locus_tag="PLX80_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19546.1"
                     /translation="MKKILLSVILLALVVYSGCGTKGMESYDAGVNLFQEKKYNECVV
                     EFQNATVENPDFSEAYMYLGRAYNSLQKYDLSIENYQKALGLFKQGKFNVKVGRLNDE
                     EKITQTEEVWMPYSQLQQKIQKGIPLTDIEIQKQEEIIKKLDPK"
     gene            7975..8928
                     /locus_tag="PLX80_01735"
     CDS             7975..8928
                     /locus_tag="PLX80_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY19547.1"
                     /translation="MEKILVIGAAGQIGSELTLALRKVYGDSNVFATDIKDAPKDVKK
                     SGPFQLLDVMDDKRLIHFVIRYKITQIYILAAVLSGNAERLPLQAWDINMRSILNILD
                     LAREVGIGKIFWPSSIAVFGTTTPKADTPQLTVMEPNTVYGISKLAGERWCEYYFNKH
                     GLDVRSIRYPGLISYKTEAGGGTTDYAVEIFDDAVESGKYECFLKPKTKLPMMFMDDA
                     IRATIELMEADSDKLTIRSSYNISGISFDPEELAGEIKKSIPDFKIIYKPDFRQAIAE
                     SWPQSIDDSVARHDWGWKHKIDLEKMTKIMLKETRKKFKIK"
     gene            8925..10367
                     /locus_tag="PLX80_01740"
     CDS             8925..10367
                     /locus_tag="PLX80_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19548.1"
                     /translation="MKPIIIFSVFMCFLISRNCVSQILPSDSGSYSFVGNRTIIFVDS
                     SRSNRNISSLVYYPSESEGSGAPVLQGNLYPVISFGHGFTLNPNLYVSLYRHLASWGY
                     IVIAPSTETGFFPSHINFARDLVFVLKDMKRKGRSSGDIFYQVIDSLNTGVFGHSMGG
                     GCSFLAGSLDSNISAISSLAAANTNPSSISAASGIKKPVQLLSGQRDSIASYWTNQLP
                     HFNNSFPFKQIINLKGGNHSQFHLVPGLDDLVDNAATISRIEQQRLTKYYVTSFFNLF
                     LKNDTSYKKFLYGTEAQNDTAVIQQYVNFELKSLIQGFYNENLRVMRNDSVRIYIRNN
                     SSPYIIRDSSINVLNDSGYCTFGFDRTNENEKYFLQIIHRNSVETWSSVGGLTFWLSG
                     SSYDFTSDSSGAFGNNLFLKGDRYCIYNGDVNRDNVIDLQDLGLTDNDVFYFKTGYVN
                     SDLTGDLITDSDDLAITDNNALLFISAIIP"
     gene            10388..11290
                     /locus_tag="PLX80_01745"
     CDS             10388..11290
                     /locus_tag="PLX80_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY19549.1"
                     /translation="MNMLSKIIDSIPLHKKGLIYISFCALLWSSGGLFIKVLTLDAYQ
                     ISFYRSLIAAITIIIISVVSKINLKFEFDIISVLCSLMYSFILVLFVIATKLTTAANA
                     IFLQFTAPIYLLLLEPIFLKTKFDKKNMIALSFCLLGMVMFFFGKLDMTSIQGNLIAI
                     GSGISFALFTLFLKWKKQLHKTENTIIYIIAGNLLVCVFCLPMVYDKLAIDFSQTLIL
                     IYMGVFQIGISYIIFNEGIKYISATESMIIAMLEAILNPIWVYIGVGEVPSVYAVIGS
                     VIILLTIVWRNLFIKPEEKIRLLD"
     gene            complement(11319..12149)
                     /locus_tag="PLX80_01750"
     CDS             complement(11319..12149)
                     /locus_tag="PLX80_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19550.1"
                     /translation="MRIIIVLIFILICCQGHTQQDIADTLDNKVVIDTSIVSDSLKTY
                     SVNFLSLPENAEVYSDTTLLCHTPALDIKMKEGKHELKFINPRSGKYWQNSNETIKFY
                     LKSDTTFSVNFKYFYYFESEPFNASVTSSDTLMGHTPLRIFTDFKLTDNIIFRKKNYN
                     DFVYNISDYNFETGAKIKMKSKGKVSLNEGVYKNKGTQFNSERNLPAIIGLGMASVAA
                     AYFTYDFKTNGNKAYDEYLLTGNQAKLDESSSDDTYFIISLLVMQAAIGGLVYFLFFD
                     "
     gene            complement(12151..13338)
                     /locus_tag="PLX80_01755"
     CDS             complement(12151..13338)
                     /locus_tag="PLX80_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HQY19551.1"
                     /translation="MIAFRYISLILVFIFAFILAGCFSLSIDNKIEVNPEEDWLMVGG
                     NIQKTNISKSKSTLAPPFNLYWDFNADGGLAKNCISVSDAIVFVNTLNGESFAIDLLS
                     GKSLGRTSTIGDASFSTPLINNSSVIITSAGEDKNRIFSYNLLSGIQKWNRNIGSVES
                     SPIMMDKDLIVCSVNNKIYRLNLQTGFIVWIARPEDSYFPLNSFYNSPTVFDNKVFAG
                     NNSGNMYAFDINTGKQLWNYKTDGPVTNDVSAKDGKIFFGSDDEYFYCLDTAGTLIWK
                     NKLNTKFSAASTFYRNYVITAGIDGNVYALNIENGDVEWTFTTNGAILATPLCHNDMI
                     FVGSYDKNFYCISADNGDKLWQYLCEGRVRTSAVIWKDYIFTASDDKYVYCFSNNEMK
                     KEK"
     gene            13894..15036
                     /gene="bshA"
                     /locus_tag="PLX80_01760"
     CDS             13894..15036
                     /gene="bshA"
                     /locus_tag="PLX80_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HQY19552.1"
                     /translation="MKIGITCYPTYGGSGVVATELGKALAMRGHEIHFISYAMPTRLN
                     GFVDNIFYHEVEINNYPLFEFPLYSIALASKMVEVTKFHDLDLLHAHYAIPHATSAFL
                     AREILNSESSTKTDMKFITTLHGTDITLTGLEPSFLPTMKFSIQKSDGVTAVSKFLKD
                     KTQQQYQIDKEIEVIPNFIDINKYKREDNEKTKCFRKNFAKNDEKILTHTSNFRALKR
                     VRDVITIFNEVKQKVDSKLLLIGDGPERSECERYSRELGIFEDIKFMGKQDALVELLS
                     VTDLFFIPSQSESFGLSALEAMSCGVPVISSSVGGLPELNLHGETGYIAEIGDVERMA
                     KYAVELLTNEKRYEQLSANARKRAEYFSEENIVPMYEKFYDKVLGM"
     gene            15183..16709
                     /locus_tag="PLX80_01765"
     CDS             15183..16709
                     /locus_tag="PLX80_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY19553.1"
                     /translation="MKTLSDEKKQVSSNRKTNGRSNGLSASINSKSKKPKWEYSKSIE
                     DKNHINLKEKYDLFIGGKWVKTKKYFETVNPATEEILARVAYATESDVNKAVKSAEKA
                     YDKVWSKMPPKERGKYIFRIARIIQEKAREFAVIETLDGGKSIKESKSVDVPLALAHF
                     FYYAGWADKLDYAFPGRKVSSIGVAGQIIPWNFPLLMAAWKIAPALACGNTVVIKSAE
                     TTPLTLYKLAEVIEEAGLPEGVVNIISGDGKTGAAIVGHPMTKKIAFTGSTEVGKLIM
                     RQTAGMDKKLTLELGGKAANIIFEDAPIDAAIEGIINGIYFNQGHVCCAGSRLLVQES
                     VKDIVIKKLKQRLSTLRVGDPMDKNTDVGAINSKMQLEKINELVAAGINEGAEIYQSQ
                     CDLPDRGYWFRPTFFSDVAQSHRIANEEIFGPVLSILTFRTPSEAVEKANNSCYGLSA
                     GVWTDKGSKIFKTLSKLRAGVVWSNTYNKFNPASPFGGYKESGFGREGGRQGLEGYVI
                     "
     gene            16945..17721
                     /locus_tag="PLX80_01770"
     CDS             16945..17721
                     /locus_tag="PLX80_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19554.1"
                     /translation="MTVTNKNIIEVKDLVKKYGKLTAVDGISFNVREGEIFGLLGPNG
                     AGKTTTLEIMETLREKTSGEVFINGLSVDKDKDQNEIKKIIGIQLQAAGYYPNLHLDE
                     LIELFAGLYNVDIDPDELLKLVDLNEKKKSKFKELSGGQKQRFSICTTLINEPKIIFL
                     DEPTTGLDPQARRNLWELIQRIKSKGTTIMLTTHYMDEAEILCDRVGIIDNGKIITID
                     TPDNLIDELVRNGFERPKQVKLASLEDVFINLTGHAIREN"
     gene            17768..18859
                     /locus_tag="PLX80_01775"
     CDS             17768..18859
                     /locus_tag="PLX80_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY19555.1"
                     /translation="MDKNYSQIRAMLSLTKASLTALMRNPSAVVFNLVFPLIFIIVFG
                     FIGGGGFKIDVVIEKNSDTLNPVYQELIKSSTIRVNKDLDDVKAMSELEKGRIDAIIK
                     IEKDPAGQSVIFLKTTKASPERGSILKMIVNEIVDKQNLAMSDLKIPKTELKEEVVEG
                     RKFKTIDFILPGQLGFSLLSAGVFGTAFIFIGLRQTLVIKRFFSTPVKKVYIILGECL
                     SRLIFSIFTSLIIIVIGYFFFGFTLVNGFVTFFNMMIMSAIALIVFLGFGFVVSGIAK
                     NESAVPSIANLITLPQFLLAGTFFPISNFPEWLQPVSRALPLTYLNEALRKISFEGAS
                     LFDVRYDILIMAAWGIVVYALAVKVFKWE"
     gene            complement(19035..19598)
                     /locus_tag="PLX80_01780"
     CDS             complement(19035..19598)
                     /locus_tag="PLX80_01780"
                     /inference="COORDINATES: protein motif:HMM:NF006168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HQY19556.1"
                     /translation="MSHILITGGTGMLSGAVNKLLTEYEAVSVIARHESGFKKILSKT
                     RHPERLNPILLDYSDTDLLSEKIISSIQNYGHIETAVNWIHSHAESAHEVIGKILDSQ
                     YISSDYFHILGSAYYNPENLSSDIEEVFSSYENISYKKIILGFKTDEAKSRWLTDEEI
                     SDGVYQAVRSAENVSNIGLTEPWERRP"
     gene            complement(19611..22142)
                     /locus_tag="PLX80_01785"
     CDS             complement(19611..22142)
                     /locus_tag="PLX80_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQY19557.1"
                     /translation="MKQSKKPRIPKTGAAPVKRNSSKEDITISEDFKKQLLGFLIGVF
                     GIMICLSILSYSDKDQATLERMSFLNIFKKESYSSAFVTYNWLGIAGAYLSDFLVNYF
                     FGYFSLIISVILITFGITLLFKKPLNRTVQFSIYASLLMMMTASFFGLLRIFTGPQFI
                     STKMSGTTGDYFASIFYHTLGSVGSTVFFSISIILLGFLLVDGNIIKTLVRFKLFFEN
                     VFDKFKREKEDLVTQKELKSNKIKNNIPDDVPVDDADFEIALKKERERILKNKYSKTV
                     NENELIKETEIYRPKQDDPSSIVIKPKKTQELSQPVLINAAGAQEIKNEMDVNPQNSN
                     DVQNLQSKKEEDFKEEKLENYKYPTLELLQEPTKEEFEVIPNEELTENGRLLQAKLLK
                     FGIEVEKVFATPGPVVTLYELVPAADVKLSKIESLQDDIALAMKAKGIRMIIPIPGKG
                     TVGVEIPNKKAQLVRIRSSFVSQKFNDSKKQLPVAFGKTIDGEVFIDDLAKMPHLLIA
                     GATGSGKSVGINTIIASLLYKLHPSDLKFVMIDPKKIELSLYSKLKKHFLATSGDINE
                     SIITSPANAVSVLKSVEREMENRYDKLANAGVRNIEAYNEKYANGNLTDNEVIRHKKM
                     PYIVVIIDELADLMITAKREIEEPIARIAQMARAVGIHLIVATQRPSVDVITGVIKAN
                     FPVRIAYQVASKIDSRTILDMGGADQLLRNGDMLYLSSASSKPIRIQNAYITTEECEK
                     LAEFIEAQEGFSKPYLLPSIIERKSITYGSEEDRDELFGEAAKLIFKLQQCSASTLQR
                     RLRLGYARAARIVDQLEEAGIVGPKDGSNARDVLITTEDELMEYL"
     gene            complement(22171..23001)
                     /locus_tag="PLX80_01790"
     CDS             complement(22171..23001)
                     /locus_tag="PLX80_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HQY19558.1"
                     /translation="MKSIKILLTQSLIQDDLMLKDKNIIILDVLRATTTMTIALANGA
                     KEIVPTENIATAVRVARGSKNSLLCGERNGKVVDGFKLGNSPSEYTTEVIKDQGLIFS
                     TTNGTLAIIKSKFAKSCVMGSFLNLSALVDYVNVIDEDFIIICAGKLNEFCLEDTVCA
                     GLILNKLSAGRELELGDSEIAAMNLCNDLAMLLNVPSQEKILRMLNISEHGKYLTSIG
                     FGADLETASKLDSYPFIPLFRNGVVKFKEMFEGESSQKSKMRRVNIGVKKEEVKEKKA
                     "
     gene            complement(23081..24160)
                     /gene="gcvT"
                     /locus_tag="PLX80_01795"
     CDS             complement(23081..24160)
                     /gene="gcvT"
                     /locus_tag="PLX80_01795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQY19559.1"
                     /translation="MKKTAFNNIHKSLGGKLVEFAGYEMPVQYEGIIAEHKAVRESAG
                     IFDVSHMGEVEVRGSDALDYLQKITTNDVSKLEKGDVQYSAMCLKDGGIVDDLLVYNF
                     GSHYMLVINASNIEKDIKWMEENLFGDVTLKNISDDTSLLAVQGKNSIHILQKLTDAD
                     LSQIEYYKFVECKLAGQDVVLSHTGYTGEKICFEIYSSSDIAKSEALWNAIFEAGKES
                     DLKPAGLGARDTLRLEYAFRLYGNDMNESNNTIEAGLGWITKVDKGDFNGKEAILHAK
                     ENPEKKIVGFIVEDRLPARQGQEVFSGDEKIGMVTSGSLSPMLNKNIGMAYIDKGFNK
                     IGTQIEIKVRDKKIKATITKTPFLQ"
     gene            complement(24193..24843)
                     /gene="fsa"
                     /locus_tag="PLX80_01800"
     CDS             complement(24193..24843)
                     /gene="fsa"
                     /locus_tag="PLX80_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQY19560.1"
                     /translation="MKIFIDTANLDEIREAAAMGILDGVTTNPSLVSKEGHKDFRSML
                     EKICAMVDGDISAEVVSTNCDDMLKEGRELAKIHKNIVVKVPLIKEGLKAVKVFSEEN
                     IRTNVTLCFSASQAVLAAKAGAYIVSPFVGRLDDISQNGMELIGQIVQIYGNYNYQTQ
                     VLVASVRHPIHFVESCLLGAEIATMPFKVIEMLAKHPLTDMGLDKFLSDWEKLQGK"
     gene            complement(25348..25998)
                     /locus_tag="PLX80_01805"
     CDS             complement(25348..25998)
                     /locus_tag="PLX80_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY19561.1"
                     /translation="MNRKSENFRIHVSVVEDDKLIRESLVEMINEAGGFSCIGNYGDC
                     ESALKDIGKNKPDVMLMDIELPGISGIEGVKKIKEIYPKIDIIMLTIHEDLSLVFKAL
                     SAGASGYLDKSATEEKILESIREIYEGGAPMSYKIAKLVVTSFQKKTESPLTSRETDV
                     LDQLCKGNSYKEIAFNLFITVGTVRHHIKNIYYKLHVHSKSEAVAKALKERLVDNS"
     gene            complement(26009..29167)
                     /locus_tag="PLX80_01810"
     CDS             complement(26009..29167)
                     /locus_tag="PLX80_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQY19562.1"
                     /translation="MKKINIYIIPVIIFITLLFRSITAQENQIKNNTFQNQIKIEHIS
                     NDLGIPTNLVFEIYQDSKGFMWFGTMFGLVRYDGDNYKTYRHDPSDKNSLSNDDIISI
                     FEDKDGYIWIGTYNGGLNRYDRRSGNFTRFFYDPNNENTVSDNTIWDIIQDKNGTIWF
                     ATESGGLCKFENDLFTSFKHDSTNSKSISGNKILTLSFDNDGNIWAGSTTGLNKLDVK
                     KGEFTVYKFDKENENSIGSNVINSLLCDSYGDIWAGTGGGGLNKFERGTGDFKRYVVD
                     SGDVNSISSNVVISVLEEEAGVLGVGTGNGFNRFYVREGKFEKVKLYPDKEFNREVIT
                     RMYKDNSGVLWLSNYQEGLHKVIRGNGKFQNYLENKSVHCFYENPDGTIWIGTTQGLL
                     KVDNNFNILDTYLNDKNNQNSISSNFVRSLTKDINGNLWIGTSFGLNKYNESNKTFTK
                     YLNSPEDEHSLSNNGISRLLTDNAGKLWVGTDFGLNEYLPAEKKFKRYLHSPTDMESL
                     SENSILSIYEDKSGSIWVGTYSGLNKMDRATGKFSHFSHDPLNDKTISNNYIYSFCED
                     ASGNFWIGTGGGLNKFDPATESFLYFIEKNGLDNSVIAGIVQDVNGNLWLSTINGLSL
                     FNINNNDFRNFNTSDGLSDNMFNFSSYMKTSDGRILFGSVNGVTSVNPADIINSSFTP
                     QVLFTDLIRYNENSKFTEDISALKDVSLSYKDNVINIGFSSSDFTHPDKIKYSYMLEG
                     FDKDWIQSGKNRNAVYTNLDPGNYIFKVISTNSDGVKNESVSELKFSISPPFWKTWWF
                     YTIILSLLIFSIILIQNIRVRSKVNHLLELEKVRESEREKVREQASRDYHDELGHKLT
                     RISLYSRRVNKKLQAFTNGISEDLKFIVETSNSLQSGAKDLIWTMNPQEDTLYDFAVR
                     LKDFGNELFDNNGMEFHSGEISEELREIRLSMNCKRHLIYIFKEGMNNILKYSECENV
                     KLNFRIYDEDVEIILEDDGIGFDPANCQKGYGLKNIFSRAAQIDLNVNISSMPGAGTK
                     ITLTAGIQNLISVFTENKSSDS"
     gene            complement(29314..30084)
                     /locus_tag="PLX80_01815"
     CDS             complement(29314..30084)
                     /locus_tag="PLX80_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY19563.1"
                     /translation="MNNRWTLKNKKALITGATRGIGKAITEEFLSLGAEVFIVSRNEN
                     DIKADLEKYTKEGHKADGMKCDVTDAGDRKKLNGKILEISDRLDILVNNAGSNNRKST
                     LENSEEDFDYISSLNMKSVFELCKIFHPMLKNSGSGSIVNIVSVAGFTSVGTGSPYAV
                     SKAGIIHFTKYLAVEWAKDNIRANSVAPWYIRTSLTEPVLSKKEYLEGVLARTPMKRV
                     GEPEEVAAAVAFLSMPAASYITGECIAVDGGFLKYGFS"
     gene            complement(30099..31583)
                     /locus_tag="PLX80_01820"
     CDS             complement(30099..31583)
                     /locus_tag="PLX80_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19564.1"
                     /translation="MNTNTELFDLIKSLTSSEKRYFTLNASVQKGNKKYLKLFDLIDS
                     QRSNDEKTLRSLKGKEDITKNFNFTKNYLTKLIFKSLLNYKNEKSTDAKLFNMLQRCR
                     ILFDKALFRQYFKTVKAGKQLAEKNERFSFLIEFMEIERQLTKKEELSGVNIEEVFDD
                     ELKILNKIRDINNYKKTVSLLFRFYRTKGMVRSEQNEKDLDECMNDLLIVSETVSDGM
                     GMSVTAKERYLFAMSMAEEIKGNFDASYNYSTKRFKLISNNINVFDESLFDSYKDSVL
                     SAVYSASISRKFAKAEKHLDSLKINSGKTEINDLNIMLSELHYNLLRVLNGKVNKNKN
                     TILAETEKFLKRNKNKITINTFNLIVYRLSAIDFFEKNYNECLRIFGEYSLEKNSGLS
                     PFIEPYARMLLILIHFEMKDHKLLQYMIPSAKKYLRSRNALHKTEDTVLNFISKMSKQ
                     NKNTNQKTLLSELTNDLIRFKKDKYEKNAFEYFDYLRWIKNINY"
     gene            31797..33581
                     /locus_tag="PLX80_01825"
     CDS             31797..33581
                     /locus_tag="PLX80_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY19565.1"
                     /translation="MKTLITIFLITFTALTHLKSQDFIKIEQSIVSLDSGASRSVNWI
                     DYDSDNDLDLFISNGKQGGENNFLYRNDNGTFVKIENDPVVTDFLPSDGSSWGDFDND
                     GFPDMCVVNWYNRINPLYKNNGNGSFTLLSNSPVSLDPSYSETCSWGDYDNDGLLDLF
                     VTNSAGTNHKNFIYKNTGGGNFIKIDTGAVFSQTAFARGVNWVDVNGDRLPEIFVSRE
                     NNQNEQFFKNNGNGYFTAITNTPLTSGGGESWSASWGDYDNDGDLDAFVTNTNNQLNS
                     LYRNDGDFTFTKITNDPLVTEQGYNAVGGWGDYDNDGDLDMFISQAYKGPTFTEKLVN
                     KLYKNNLMETGTASFEKLTSGVLVNDSGYTFGFAWGDYDNDGDLDLAAANTFNENQKN
                     ALYRNELSTGNKWINIKCTGTTSNKSAIGTKVRIKANINGNSVTQMQEISGQAGYCTQ
                     NLILHFGLGNAAVIDSIKVEWPSGEDQYFTNVPVDQNITITEDGTLISINENEISVVK
                     DFQLFQNYPNPFNPNTRISYRLNKNSKVLLQIFDTLGNEITTLVNDSQNEGEYESEFT
                     GNNLSSGVYFYKLTVNEISQTRSMILVK"
     gene            complement(33751..34206)
                     /locus_tag="PLX80_01830"
     CDS             complement(33751..34206)
                     /locus_tag="PLX80_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HQY19566.1"
                     /translation="MELFRISSEKYSKGLVSSGRPNRWNKNGEHVIYTGGSRSLSTLE
                     LIVHRNFIKPDIIYKVLIISVPDSENIIKTVKTKDLPENWRKLEAYSKLQEIGSDWIQ
                     SKETLLLKVPSAVIPQEQNYIINIEHSDFKRKVKLVRSEEYFWDEGLLQ"
     gene            complement(34295..34570)
                     /locus_tag="PLX80_01835"
     CDS             complement(34295..34570)
                     /locus_tag="PLX80_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19567.1"
                     /translation="MLFGLSVLTMIVNGIALSAIVFRIYEWGTYPNKLAVLVGNILIL
                     INLLFVTFRLYKTIKDKNEIYRVEKSMALFLPVYSLWTALVVMKKHR"
     gene            complement(34740..>34936)
                     /locus_tag="PLX80_01840"
     CDS             complement(34740..>34936)
                     /locus_tag="PLX80_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19568.1"
                     /translation="IFKNDEISWEKTNELKFVVLLSLLAGFLAKLPEMIGMDAEYFYQ
                     RNLSLIVFPFGYHILHGSKK"
BASE COUNT        11198 a         6164 c         6152 g        11422 t
ORIGIN      
        1 tagttaatag ttaatagtta atagttaata gttaatagtt aatagttaat agttgatagt
       61 taatagttaa tagttgatag ttgatagttg atagttgata gttgatagtt gaaatagatt
      121 ttataaaatt atgaagttaa ggattaaatt aatttttttt taaaaaacct cttacacaac
      181 agactgtgct aaaacaaaac tattaagttt acctcgaagg atcacaggag ttaagtatta
      241 aattaatttt aactttgagt cttagagcct ttgaggcaac ctcaacaatt tcagtcatta
      301 taaaaggttg gtcattatga tgatattgtt taacagattt attaattttc tcacaacatt
      361 atcatttgtc tgcataatat taatttcaat tgataaataa aattatataa gtaattagat
      421 ttgtaattaa aatctaatta aatattttgg ataaaaattt aaaataattt tcgagcctgt
      481 ttgaaaaaag tataccaaaa atttaaagat tacaatacga gaagaaatgt tttaaaatac
      541 aggctgtata aatttctaaa aaaacattcc aaagtaacag aagactcccc ggtcattatt
      601 gtctgggaca tgggcggatt tgcagacctt ttaaaaaaga atgcgatgtt tgcagcagct
      661 ctgaatctcc gaggatataa aactcatttc attatttgtg acggaactcc cgaagcctgt
      721 attcagcggg gacttgagaa aaatgaaaag gtcgaagact gggacaagaa atgtgtgacc
      781 tgtacaaaag ctatggaata ttattcacgg gaatattcag cggagtattc tcatgcaggt
      841 gattatattt cctccgaaaa aaaggatgaa tttcttaacc tttcaaacac aataaagatt
      901 gaagatattt tcgcttataa atttcttgaa ttaagtgtcg gctctttagc ctggagctct
      961 gtgataagat atatgaaagg atatgtgatt gagcttaagg atcttaaaaa agaagatgag
     1021 atcatttaca gaaaatattt ttttgctggt ctggtaaata cttttattgc aaatgaagta
     1081 ataaataaat tcaaacctgt aagtctttac agctcacacg gtgtgtatgt ggattattcc
     1141 ccgcctgtcc tgcttgctta catccgaggt ataaagacac tttgctggtc gagcggattc
     1201 aaggattttc ttcattattt tacaattcct aaagacagaa acaagcttga attcagagga
     1261 attacagaac ctgaatggaa aaaaagagca gccgtttctt tgactgaaaa agaaaatgaa
     1321 tttctttatt catatatatt tcacaggttc aataaaggaa aaggaaaaga ttttctgaat
     1381 gtaacacttc ctgaaactga agatcagctc agagagaaac ttggttttga aaaagggaag
     1441 aaaattgttt gtcttttttg tcatgtaaac tgggatctca catttgatct ttcgaagatg
     1501 gtatttgaca atgccaatca gtggctgtct caaacactcg aagcaatttt taaaaatgat
     1561 aaagtaaact ggatcatcag agtgcatccg ggtgaaaaag cttccggaag tctttataca
     1621 aatgatgatt ttttaaaaga gagatatccc gacatcccat ctcatgtgcg gatcttatgg
     1681 tcagattctg agatcaattc attaggttta tataaattac ttgatatagg gatcacatta
     1741 tttggaacta tgggttcaga gctccctctt tttggaaagc cggtaatctc agccggcgaa
     1801 gcacatttta cggggaaggg gttttccatt gatactaaga cccgtgagga atatttcaaa
     1861 tgcattgaaa ctatacagga cattgaaccg ctcaatcccg ataaaattga gatagccaga
     1921 aaatatgcat attcatattt cgtacaacgg caggtacctc ttggtattat agttaaaagt
     1981 gaaggacatt tcggaaatct ggattacaaa aaacttgatc accttttacc cggtaaagat
     2041 cttatcatag atgcgatctg cgaaagcatc atcgaaggta aagatgttat acttgatgag
     2101 aaaatgctga aagtttctga acaggatatt tctttggaaa taatgccaat acaattaaat
     2161 gtataaattt tgatagttaa tagttaatta tgaaaatttt atccttattc ctgaaattgt
     2221 gaatttggta atgtaaccaa aaaatatttg ctcccgcaga ctcaagtgct ctttggatta
     2281 tactgataca gaatgaaaat tattctggtg tccgtaaatt ttaaaatcat aacaatgaaa
     2341 aataattttt tattttactc tgtctgtata actatattat ttttatcttc atcagtattt
     2401 tcccaatcaa aaattgttac gggtacatat aaaggcgagc cgttagcata ctataaagat
     2461 aaattaatag tggcttacct tacagatagt gtaaataaca ttgaatacat tcacagagta
     2521 aatgacttta taagaaagca caacagcagc attgattcgg taatttcaat tttacagtac
     2581 agggaaatag atattacttt ttattcaaat aatgctgacg ctgttgatct catagatatt
     2641 tttaaagaca gtggtctgtt tgagtttgtt agtctgtcag gtctcatagt accagaaatg
     2701 ataaagcctg aagattaagt gaaaagtgaa tagtgtataa ttttatctgt atttctgaaa
     2761 ttgtacaatt tgtaatttgt attcaaaaaa tatcgactat taacattcct gtttatgata
     2821 ttttgttaaa aatcagataa tcatttaccc catatgaaaa caataatgat tattcatgag
     2881 ctcacttaaa atatttatta aaattctgat tcaaaataag ttttgattat gtcgaaaaga
     2941 atttctgtaa taataccaac ttataacaga gtgggtacac ttgggataac tataaaaagt
     3001 cttatagaac agacttatcc tgataatctg tatgagatca ttattgcaga taataattct
     3061 agtgataata ctaaggaatt ggtagagagc attgcaaagg actctgctgt acctgtgaaa
     3121 tatttgtttg agaaaagacc tggagctcat tttgctagga atacagcagc tcagatgtcg
     3181 gatagtgagt acttatattt tacggatgac gatatgattg ccgatccgga tatgctggaa
     3241 aatattttaa aagtatttga tcttgattac aacattgcag tgactggcgg gaaggtattg
     3301 ccgaaatggg aattcgatcc tcccgaatgg ctgcttaaat attttcagga cggtacatta
     3361 agtttaataa ataagcctga aaagttaata attaccaatc atgactttgg aatatatagc
     3421 tgtcatcagg caatgctcag gagtgtgttt ttcgaatgcg gcgggtttaa tcctgatatt
     3481 gaaaaagata aactcattgg aaacggcgag acaggactga atattaaaat ccttaaaaag
     3541 ggatataact ttgcatatac tgctgattcc gttacgaggc acattattcc aagatcacga
     3601 atgacacaaa aatatcttaa caacagattc agcaatcagg ggatctgcga cggttttaca
     3661 aatttcagaa aatccgaata ttcaaaaaaa aatcttttca gacttatttt taattctcat
     3721 ttacctttgc ttctgaaaag tttctccgac tcttttttaa atcagataaa gggaaacagt
     3781 ttatggagac tcaagcgtgc aaattttcat taccattaca acagaataaa atttgatatg
     3841 aaacttattt caaatgacga atggagaaag tttgtactga gaaatgaata ttttaactac
     3901 tgatatttac acagtcattt tattctgact taattttatc ctgccgaata agaaataaaa
     3961 aactactaac attgtccccg atgctgagaa caggaacatt gcctccggat taaaattata
     4021 ccaaatcaaa cctgcaatta cacttgctgc cagtgagaaa agactgttca gtcctgtata
     4081 aaaaccaaga gcagtagcag tatctttctt ttgtgaaata tttgtgatcc atgcttttga
     4141 aacaccttca gtaaatgatg catacagacc gtatgcaata aacaatatat ataaaaagac
     4201 ctgatcagga cctaaagcca ttcccgcata tacaaataca aagatcagaa gacctgctat
     4261 gaaatttttt ctgaagccga atttgtcagc aattatacct gacggataag atgtcagcgc
     4321 atatacaaga ttataaaaaa tatatatcca tattactttt tcatcggaat atccgagatg
     4381 ctttatcatc agtagaagaa aaatatctga gctgttaaat aaagtaaaag cgaaaagtcc
     4441 tttcacgagt tttttatatt caggtactga ctctttccaa tatccgagga atccaaaaaa
     4501 attatactta gatgctttgg gtcttttgat tatttcacta ttatcttttt ctttttcttt
     4561 aattaaaaat gtaaacagca ctcccaatat tgccggaatg aaagcaataa aaaacaatgt
     4621 cctgtactgc tccggatata tgctgagata tatcagcgca aggaacggac cgattgcagc
     4681 tccgagagta tcagcacctc tgtgaaatcc aaagaccttt gctttattct caggtaaagt
     4741 ttctgaagaa agtatcgcat cgcgagctga tgtccttatc ccttttccga gtctgtccat
     4801 cgtacgcgcc agaaaaatcc acacaggata gacaaataat ccaagcatcg gttttgaaag
     4861 tgagctaagc aaatacccta ttgtcacgaa cggtgtccgc tttcctgtga catcagactg
     4921 cttaccgaaa tatcccttgc tgagacctgc ggtagcttcg gcaatccctt cgagaattcc
     4981 gatgagcagt acagaaaacc cgatgctctt cagatagatc ggcataacgg gataaagcat
     5041 ttcactggaa atgtcagtaa ggaaactgac tgccgagagg agaagaatat ttttggtaag
     5101 gatttttttc agcacattga aatgacagta aattttattt cagtaattta tataaatttg
     5161 aattaagatt ttagtgtgaa taagttacgg ggagaagatc tgtgtttttc aaaatttttc
     5221 tgcctttaaa aatttagttt cttaataaga attattctaa attcattcaa taaattcatc
     5281 aagatatgaa agctccagcc aaaatatttc ttgcattaat tttttacttt tctctaagta
     5341 cttttctttt tcaaaattct tttgctcagt tggtttcgga ttttcgtgtg aatgatgata
     5401 gtaccaatac ttcacaatat ggagctaaaa ctggagttga tggcaaggaa aattttttgc
     5461 aatagtttgg acagatttta gagattaaaa atcggatggt gttaatgttg atatattttg
     5521 tcaaatattt aattcaaacg gtcaaatcat tggtaataat tttagaatag atgagttagc
     5581 cggatcctca agctctcctc ttatctcagt tagaagagat ggaagttttt tagtttgctg
     5641 gaaggatttt agtatcaagt ataggttttg aaacatcctt ctttaaatac agatttattg
     5701 aaaactcaat ggagagggat atactcgttt agaatagata agaaatacag agcattattt
     5761 tttatagcaa atggtaaagc ggaagtattt cagataacta atcattatag aaaataaaaa
     5821 agctgtaaag ttgacaattc atttaaccta agaaaaaaag ccaataggtg ctttcgagaa
     5881 ttttattaaa atgactaaag ataatcccca tttccattca taaataaatt gttacacaca
     5941 atttatttaa ttaatttgtc atattctaaa aaaatcgatg caaattcaat gaaagataaa
     6001 atacgtatag cttccgggca gggattctgg ggagacctgg tagatgctcc ttataatcag
     6061 gcaaccggcg gacccgttga ttatattatg atggattatc ttgccgaagt tacgatgtcc
     6121 attctccaaa agcaaaaact cagagatcct gaattcggat atgcacgcga catccctccg
     6181 ctcatggaga gattacttcc cgttataaaa gaaaaaaata ttaaggtcat cacaaacggc
     6241 ggcggcgtaa atcccgttgc ctgcagagat gcaattttta aagtcgctga aaagatcgga
     6301 gtcaaaggtc tcaagatcgg tatcgtacag ggtgataata ttcttgataa tatagatgat
     6361 cttgtttcaa aagggatcga acttaaaaat atggaaaccg gtgaatctgt aaaaaccgtc
     6421 cgtgataaaa ttctcagcgc aaatgtttat ctcggagcgg ctcctgtggt cgaatgtctg
     6481 aagcagggtg ctgatattgt aatcaccgga agggttactg atacagggct tacacttgca
     6541 ccgatgatct atgaattcgg atggaatatg aatgactggg atctgatggc ttccggaacg
     6601 gtagccggac acattctcga atgcggcggt caggcgagcg gcggaaattt tctcggcgac
     6661 tggaagagca ttcctgatct tgcgaatatc ggatttccga ttgctgaagc ttttccaaac
     6721 ggagaggtca ttatcacaaa acatgaaaac accggcggca gagtcagcat tgaaactgtc
     6781 agcgaacagc ttgtttatga gatcggcgat ccgaaagatt atattactcc agactgcata
     6841 gcggatttta cttcaataca gcttgaagat gtcggtgaaa acagagtcag agtttttggt
     6901 gtgaaaggtc tgcctgccac tccatttttt aaagtctcaa tgtcatactc ggacggatac
     6961 agcgctgttg gttctcttac atattcatgg cctgatgcac ttgataaagc caaagctgct
     7021 gatgaaattt taaggactcg tttaagtaat ctcggattaa aatttgatga gatcagatct
     7081 gaattccagg gatacaatgc ctgtcacggt cctttggcag aaaagtttga tgatctgaat
     7141 gaagtcgtac tacgatttgc tgtaaggtcg caggataaaa gtgctttgga aagatttgga
     7201 aaggaaattg ctccgctgat tcttacaggt cctccgggtg ttaccggatt tgcaggcggc
     7261 agaccaaagc cgtcagatgt agtagcatac tggcctgcac tgataccgaa agaagaagtt
     7321 gagtcaattg ctgaaaccat gagtaattaa aagttatgag ttataaaccc caccccgcct
     7381 cccctttgga caaagtggag gagcaataaa agtttatcag tgcatttaat tttgaaaatg
     7441 tcattgaaag aattaaaaat tagtttaaat gaaaaaaatt ttattaagtg taatactttt
     7501 agctttagtt gtatattcag gatgcggaac taaaggaatg gaaagctatg acgcaggagt
     7561 aaatctgttt caggaaaaaa aatataatga atgcgttgtt gaatttcaaa atgcaacagt
     7621 ggaaaatcct gatttcagcg aagcctatat gtatctggga agagcatata attctctgca
     7681 gaaatatgat ctctcaatag aaaattatca gaaagcactc ggtcttttta agcagggaaa
     7741 attcaatgta aaagtgggca ggctcaatga tgaagaaaag atcacacaga ctgaagaagt
     7801 ttggatgcct tattcccagc tacagcagaa aattcaaaaa ggtattcctc ttacagatat
     7861 tgaaatccag aaacaggaag agataattaa aaaactcgat cccaaataat tttaaacacc
     7921 ttcaatattt ttttgtacta taataatcat tcagatataa aagataattt ttaaatggaa
     7981 aaaattcttg tcatcggagc agctggacag atcggctccg aacttacctt agcactaaga
     8041 aaagtttacg gagattcaaa tgtatttgct actgacatta aagacgctcc taaagatgta
     8101 aaaaaatccg gaccgtttca gcttctcgat gtaatggatg ataaacgttt gatacacttt
     8161 gtgatcagat ataagatcac acagatctat atacttgcag cagttttatc aggtaatgct
     8221 gaacggctgc cgctgcaggc atgggatata aacatgagaa gtattttgaa cattcttgat
     8281 cttgcaagag aagttggtat tggaaaaatt ttctggccaa gctcgattgc agttttcgga
     8341 acaaccactc caaaggcaga tactccgcag cttactgtca tggagccgaa tactgtttat
     8401 ggtattagca aactcgccgg cgaaagatgg tgtgaatatt attttaacaa acatggactt
     8461 gatgtaagaa gcatcagata tccgggtttg ataagttata aaactgaagc aggcggtgga
     8521 acgactgatt atgcagttga gatatttgat gatgcagtcg aatccgggaa gtatgaatgt
     8581 tttctcaaac ctaaaacaaa acttccgatg atgtttatgg atgatgctat ccgtgcgact
     8641 atcgaactca tggaagctga ctcagataaa cttacaatca gatcaagtta taatatcagc
     8701 ggaatcagtt ttgatcctga agagcttgcc ggtgaaataa aaaaatccat tccggatttc
     8761 aagatcattt acaaacctga tttccgtcag gcaattgccg agtcctggcc tcaaagtatt
     8821 gacgattctg ttgcgagaca tgactgggga tggaagcata agatcgacct tgaaaaaatg
     8881 acaaagatca tgctgaaaga aacccggaaa aaatttaaga taaagtgaaa ccgataatta
     8941 tattttcggt ctttatgtgt tttttaatca gcagaaattg tgtctcacag attcttcctt
     9001 ctgattccgg aagttattct tttgtcggga acagaacaat tatatttgtt gattcttcaa
     9061 gatcaaacag aaatatttct tcacttgtct attacccttc tgagtctgaa ggctccggcg
     9121 cacccgtttt acaggggaat ctttatcccg tgatttcatt cggacatggg tttactctga
     9181 atccaaattt atatgtttct ctttacagac atctggcgag ctggggatat attgttatag
     9241 caccttcgac tgaaaccggt ttttttccta gtcacataaa ttttgccagg gacctggtat
     9301 tcgttttgaa agatatgaaa agaaaaggca gatcgtcagg tgatattttt tatcaggtta
     9361 ttgattcttt aaacacagga gtctttggac attcaatggg aggaggctgc agttttcttg
     9421 ccggatctct agattcaaat attagtgcaa tttcatctct tgctgctgca aatactaatc
     9481 catcttcaat atccgctgct tccggaataa aaaaacctgt tcagctttta tccggtcaga
     9541 gagacagtat agcatcttat tggacaaatc agcttccaca tttcaataat tcttttcctt
     9601 tcaagcaaat tattaactta aagggaggca atcacagcca gtttcatctt gtaccagggc
     9661 ttgatgatct cgtcgataac gcagctacaa tatcccgcat agaacagcaa agacttacaa
     9721 agtattatgt aacttcattt tttaatctgt tcctgaaaaa tgacaccagt tataaaaaat
     9781 tcctttatgg aactgaagca caaaatgata cggctgtaat acagcagtat gtgaactttg
     9841 aactgaaatc tcttattcag ggattttata acgaaaatct ccgggtaatg cggaatgatt
     9901 ctgtaagaat ttatatcaga aataattcat ctccatatat tatcagagac tcttccataa
     9961 atgttctgaa tgattcaggt tattgcacat tcggtttcga tcgaacaaat gaaaatgaaa
    10021 aatatttttt acagataata cacagaaatt cagttgagac atggagttct gtcggaggtc
    10081 tgactttttg gttaagcgga agttcgtatg attttacatc tgattcttcc ggcgctttcg
    10141 gaaataatct ctttttaaaa ggcgatcgtt actgcatata taatggagat gttaacagag
    10201 ataatgtaat tgatcttcag gaccttggat taaccgataa tgatgtattc tatttcaaaa
    10261 ccggatatgt aaattccgat cttacaggag acctgataac tgattcagat gatctggcaa
    10321 ttactgataa caatgcatta ctttttattt cggctattat tccctgagaa taaagtgaat
    10381 aattctaatg aatatgcttt caaaaattat tgattcgatt cctcttcata aaaagggttt
    10441 gatatatatt agtttctgcg cactcctctg gagctccgga ggtttattta ttaaagtcct
    10501 cacactcgac gcttatcaga tctcattcta tagatcgctc attgcagcaa ttacgatcat
    10561 aattatttca gttgtcagta agataaatct gaagtttgaa ttcgatatca tctccgtact
    10621 ctgcagtctg atgtattctt tcatacttgt actttttgta attgctacaa aacttaccac
    10681 tgccgctaac gcaatttttt tacagttcac tgcgccgata tatctccttt tgcttgagcc
    10741 catttttcta aagacaaaat ttgacaaaaa aaatatgatc gcgctttcat tctgtctgtt
    10801 aggaatggta atgttctttt tcggaaagct tgatatgacg agcattcagg ggaatctgat
    10861 cgcgattgga tcgggtatca gctttgcact ttttactctt tttctcaaat ggaaaaagca
    10921 gcttcataaa accgaaaata cgattatcta tatcattgca ggtaatttat tagtgtgtgt
    10981 attttgtctg ccgatggttt atgataaact tgcaattgat ttttcacaga cacttatact
    11041 tatttatatg ggtgtgtttc agatcgggat aagttatata atttttaatg aaggcataaa
    11101 atatatttct gctaccgagt caatgatcat tgcaatgctc gaagcaattc ttaatccaat
    11161 ctgggtttat atcggagttg gtgaagtgcc gtcagtttat gctgtgatcg gatctgtgat
    11221 aatacttttg acaattgtct ggagaaattt atttatcaag cctgaagaaa agatccgttt
    11281 attagattag caaattttaa aataacggat tacctgtttc agtcaaaaaa aagaaaatac
    11341 acaagtccgc cgatagcagc ctgcattaca aggagtgaga tgataaaata tgtatcatcg
    11401 gaagaggatt catctaactt tgcctgattg cctgtgagta aatattcatc atatgcctta
    11461 ttaccgtttg tcttaaaatc gtaagtgaaa tatgcagcag caacggaagc cattccgagc
    11521 cctataatag ccggtaaatt tctttcagaa ttaaactgtg ttcctttatt tttataaact
    11581 ccttcattca gacttacctt accttttgat ttcattttaa tttttgcacc tgtttcaaaa
    11641 ttataatccg aaatattata tacaaagtca ttataatttt tctttctgaa aattatgttg
    11701 tctgttaatt taaaatcagt aaatattctt aaaggtgtat gtcccattaa agtatcactg
    11761 cttgtcactg aagcattaaa aggttcagat tcaaaataat aaaaatattt aaagttaacg
    11821 ctgaatgtag tatcactttt cagataaaac ttaatggtct cgttagaatt ctgccagtac
    11881 ttgccactcc gcggatttat aaatttcagc tcatgctttc cttctttcat ttttatatct
    11941 aaagcaggtg tgtgacagag gagtgttgtg tcgctgtaaa cttcagcatt ttcgggaagg
    12001 gataaaaaat ttacggaata agttttaaga ctgtcagaaa ctattgatgt atcaatcaca
    12061 actttattat caagtgtatc tgcaatgtcc tgttgagtgt gtccttgaca gcagatcaga
    12121 ataaatatca aaacaattat aatccgcata ttatttttcc ttcttcattt cattatttga
    12181 aaaacagtaa acgtatttat catcactggc agtaaagata tagtccttcc atatcaccgc
    12241 actggttctt actctgcctt cacaaagata ctgccatagt ttatctccgt tatcagcact
    12301 gatacagtaa aaatttttat cataagatcc gacaaagatc atgtcattgt gacaaagcgg
    12361 tgtagccaaa attgctccgt tagttgtgaa agtccattcc acatctccgt tttcaatatt
    12421 taacgcatac acatttccgt ctatacctgc agtgatcaca taatttctgt agaaagtaga
    12481 agctgctgaa aattttgtgt taagcttatt cttccagata agtgtacccg cagtatcgag
    12541 acaatagaaa tattcatcat cagatccaaa aaaaatcttt ccgtcttttg ctgacacatc
    12601 atttgttaca ggtccatcgg ttttgtaatt ccaaagctgt ttacctgtgt taatgtcaaa
    12661 ggcatacata tttccgctgt tatttcctgc aaagacttta ttgtcaaaaa cagtcggact
    12721 gttataaaat gaatttaaag gaaagtaaga atcctccggt cttgcgatcc agactataaa
    12781 accggtttgt aaattcagac ggtaaatttt attattcact gaacatacga tcagatcttt
    12841 atccatcatg atcggagatg attctacgct gccaatattt ctgttccatt tttgtattcc
    12901 gctgagcagg ttataactaa aaattctgtt tttatcctct ccagcagatg taatgattac
    12961 actgctgtta tttataagcg gagtggaaaa agatgcatcg cctatagttg aagttctgcc
    13021 gagactctta ccgcttagta gatctattgc aaatgattca ccattcagtg tatttacaaa
    13081 tacaattgcg tcactgaccg aaatacagtt cttcgccagg ccgccgtctg cattaaaatc
    13141 ccagtaaaga ttaaaaggag gtgctaaagt agatttggat tttgaaatat ttgtcttctg
    13201 aatattacct ccgaccatca gccagtcctc ttcgggattg acttcgatct tattgtctat
    13261 gcttagagaa aagcatcccg ctaaaataaa cgcaaatata aaaacaagaa tcagagaaat
    13321 gtatcggaaa gcaatcataa aaataattat tgagtaaaat aaaaatttaa tcagacaaaa
    13381 cggattgaat ttttaataaa ttttttgcgg agggttggat tgtgaactgt caagaatcga
    13441 tatttcaaat ttctttttca agatagaaat ttaataaaaa attttaaata ttaaatttta
    13501 aatagtgtta ttaaaaataa gactttacga acctgaattc attcgggtga agcaatccga
    13561 attttgtaca gttctaaaat ttaaaagaat actatttcta aaattaaaat ttgagccact
    13621 tttggtgttt caaagagttt gcgaatttaa aagtgcgatt tgaatattat ataactgcaa
    13681 atttttgacc gccaacagcc tgaaaagaag agaacgacaa ttgaaataat ttaaaattga
    13741 aacacataat attaaatatc tactatcccg cagagaatga tcaattctct gcgggatttt
    13801 taatttatcc ttataatttt taagctttat accctaatga atttttctta agacaagaat
    13861 aaattatatt tacacctata aaattttata caaatgaaaa taggcattac atgttatcct
    13921 acttacggtg gcagcggagt agttgcgaca gaacttggca aagcacttgc gatgagaggt
    13981 catgaaattc attttatctc ttatgcaatg ccgacacggc ttaacggatt tgtcgataat
    14041 atattttatc atgaggtaga aataaataat tatcctttat tcgaatttcc tctatattca
    14101 atagctcttg caagtaaaat ggttgaagtc acaaaatttc atgatctcga tctgcttcat
    14161 gcacattatg caattcccca tgctacaagt gcattcctcg cgcgtgagat cctgaattcc
    14221 gaaagcagta caaagaccga tatgaaattc attactacac tgcacggaac tgacattact
    14281 ctgacgggac tcgagccgag ttttctgccg actatgaaat tcagtattca gaaatctgac
    14341 ggcgtcactg ctgtatcgaa atttctaaaa gataaaactc agcagcagta tcagatagat
    14401 aaagagatcg aggtcatccc aaactttatt gatataaata aatacaagcg tgaagataat
    14461 gaaaagacca agtgtttcag aaagaacttt gcgaagaatg atgaaaaaat tctgacacat
    14521 acatctaatt ttcgtgcact gaaaagagta cgggatgtga ttacgatctt caatgaagta
    14581 aaacaaaaag ttgattcaaa gcttttgctg atcggtgacg gtccggagag aagtgaatgc
    14641 gaaagatatt caagagagct tggtattttt gaggatataa aattcatggg taagcaggat
    14701 gcattggtag aattactttc agttactgat ctgtttttta taccgtcgca gtctgaaagt
    14761 tttggcttgt cagcacttga agcaatgagt tgcggagtgc cggtgatctc atcaagtgtc
    14821 ggtggtttgc ctgagctgaa ccttcacgga gagacaggct atattgcaga gataggtgat
    14881 gttgaaagaa tggcaaaata tgctgttgag cttttgacaa atgaaaagag atatgaacaa
    14941 ttatctgcaa acgccagaaa aagagctgag tattttagtg aagaaaacat tgttccaatg
    15001 tatgagaaat tttatgataa ggttctgggc atgtgaagtt cattcaaaga gcagatacag
    15061 ataaacaaaa gttttatata taaaaagaca aatctaaaat ttgtaattta aaataaaaaa
    15121 atttagctga atttccatta cagatcagcg aaacagaata tctctgtaaa taaatattta
    15181 ttatgaaaac attatcagac gagaaaaagc aggtttcatc taacagaaaa accaatggaa
    15241 gatcaaatgg tttaagtgcc tctataaatt caaagtcaaa aaaacctaaa tgggaatatt
    15301 cgaaatccat cgaagataag aaccatatta atcttaaaga aaaatatgat ttgtttattg
    15361 gcgggaaatg ggtaaagaca aaaaaatatt tcgagacggt aaacccggct actgaagaaa
    15421 tcttagcgag agttgcctat gcaactgaat ctgatgtaaa taaagcagtc aaatccgctg
    15481 agaaagctta tgataaagta tggagcaaaa tgccaccgaa agaaagaggg aaatatatat
    15541 tcaggatcgc acggatcatt caggagaaag cacgggagtt tgctgtgata gaaacattgg
    15601 acggcggaaa gtcgatcaaa gaatcaaaat cagttgatgt gccgctcgca cttgcgcatt
    15661 tcttttacta tgcaggatgg gctgataaat tagactatgc atttcccgga aggaaagtat
    15721 cttctatcgg agttgcaggt cagatcattc catggaattt tcctttatta atggctgcat
    15781 ggaaaattgc acctgctctt gcctgcggaa atacagtcgt cataaaatct gctgaaacta
    15841 cgccacttac tttatataaa cttgctgaag tcattgaaga agcaggtctt cctgaaggcg
    15901 ttgtgaatat tatttcaggt gacggaaaga caggcgcagc aatagtcgga catccgatga
    15961 caaaaaagat cgcattcaca ggttcgactg aagtaggtaa actcatcatg agacagacag
    16021 ccggaatgga taaaaaactt acacttgagc ttggcggaaa agctgccaat ataatttttg
    16081 aagatgcgcc gatcgatgca gctatagaag gaatcatcaa tggaatttat tttaatcagg
    16141 gacatgtatg ctgcgcgggt tcaaggctgc tcgttcagga aagtgtaaaa gatattgtaa
    16201 taaaaaaact taagcaaaga ctttctacat taagagtcgg tgatccaatg gataagaata
    16261 cagatgtcgg agctataaat tcaaagatgc agcttgaaaa aattaacgag cttgttgcag
    16321 caggaataaa cgaaggtgcg gaaatttatc agagtcagtg tgatcttccg gacagaggat
    16381 actggttcag accgacattt ttctcagatg tagcgcagtc tcacagaatt gccaatgaag
    16441 aaattttcgg accggtctta tctattctta cattcaggac accttcggaa gctgtagaaa
    16501 aagcgaataa ttcctgctat ggattatctg cgggagtgtg gactgacaaa ggttcaaaaa
    16561 ttttcaaaac tctgagtaag ttaagagcgg gtgtggtctg gtcaaataca tataataagt
    16621 tcaatcccgc atcgcccttc ggcggatata aagaaagtgg ttttggaaga gaaggcggca
    16681 gacagggact tgagggatac gtcatctgac ctttttctga accacataga cacatagaac
    16741 acacagcaca cctgtcttcg cagagtcacg tgctcttcgg actaagcggg aaaacgggat
    16801 tgaaccttca ataaatctaa attgatatta aagtttaagt aaagagtatt aacaatttcc
    16861 atagagtcat gtgcttttcg gactctgcgg aaaaacgaga tagaaacttc tgtaaatcct
    16921 aaatgatatt catccaaaaa taatctgaca gtcacaaata aaaatataat agaagttaaa
    16981 gatctcgtaa agaaatacgg caagctcact gcagttgacg gaattagttt taatgtcaga
    17041 gaaggagaga tcttcggact gctcggaccg aacggtgccg ggaagacgac tacacttgag
    17101 atcatggaaa ctctccgtga gaaaacttcg ggtgaagttt ttattaatgg actgtctgta
    17161 gacaaggata aagatcagaa tgaaataaaa aagatcatcg gtattcagct tcaggctgca
    17221 gggtattatc cgaatcttca tcttgacgaa ctcatagaat tattcgcagg actttataat
    17281 gtagatatag atcccgatga attgttaaaa cttgtcgatc tgaatgaaaa aaagaaatca
    17341 aaattcaaag agctttcagg cgggcagaag cagcgtttct ctatatgtac cactcttatc
    17401 aatgaaccta agataatttt tctcgatgaa ccgacgaccg gtctcgatcc gcaggcgaga
    17461 agaaacctat gggaattaat tcagcgcatc aaatcaaaag gaactacgat catgctgacg
    17521 actcactata tggatgaggc ggaaatttta tgtgacaggg taggtataat tgataacgga
    17581 aaaataatta cgatcgacac acctgataat ttgatcgatg aacttgtcag gaacggtttt
    17641 gaaagaccaa agcaggtgaa gctcgcatca cttgaagatg tatttataaa tcttacagga
    17701 catgcaataa gagaaaatta agagttaatt tttcaaacta aaataatttc attccaaaaa
    17761 aaccaagatg gataaaaact acagtcagat ccgtgcaatg ctgtcactta cgaaggcaag
    17821 cctgactgcg ctgatgcgta atccttctgc ggttgtattc aatcttgtat tccctctgat
    17881 ctttataatt gtgtttggat tcatcggtgg tggtggcttt aagatcgatg tcgttattga
    17941 aaaaaattcg gatactttaa atcctgttta tcaggaatta ataaaaagca gtacgatcag
    18001 agttaataaa gatctggatg atgtgaaagc gatgtctgaa ttagaaaagg gaaggatcga
    18061 tgcaattata aaaattgaaa aagatcccgc tggtcagtct gtgatatttc taaagactac
    18121 aaaagcttct ccggagagag gaagcatttt gaaaatgata gtgaatgaaa ttgtcgataa
    18181 gcagaatctt gcaatgtctg atctgaaaat tcccaagaca gaattaaaag aggaagtggt
    18241 cgaaggtcga aaatttaaga ctatagattt cattttaccc ggtcagcttg gattttcact
    18301 gctaagcgcg ggagtattcg gtacagcatt tatttttata ggtctccgtc agacactcgt
    18361 gatcaaaaga tttttttcta cgcctgtaaa aaaagtttat attattttag gcgaatgtct
    18421 gagccgactg atcttttcga ttttcacttc tcttataatt attgtgatag gatatttctt
    18481 ttttggattc acacttgtaa atgggtttgt gactttcttc aatatgatga taatgtctgc
    18541 aattgcatta atagtttttc ttggattcgg gtttgtcgta tcgggaattg caaagaatga
    18601 aagcgcagtt ccgtcaattg cgaatctgat cacactgcct cagtttcttc ttgctggcac
    18661 tttttttccg atatcaaatt ttccggaatg gctgcaaccc gtaagccggg cgctgcctct
    18721 gacttatctg aacgaagcat taagaaagat ctcgttcgaa ggtgcaagcc tctttgatgt
    18781 cagatatgat attctgataa tggctgcgtg gggaattgtc gtttatgctc ttgctgttaa
    18841 agtttttaaa tgggaatagt tttaagagca attaaaaatt aagaattaag aattacgaat
    18901 atttttgata atgaaaatat aatagattaa ttttgcgagc aaagcaatct ccgaatctgc
    18961 cgcataaaat ccccggtttt attaatctta aattcaaaaa ttgatttgcc tgaagttcac
    19021 ttatgaaatt gagatcaggg tcttctctcc cacggctctg ttaaaccaat attagataca
    19081 ttctcagctg atcttacagc ttgatacacg ccatcactaa tctcctcatc tgtcagccat
    19141 ctcgattttg cttcgtcggt tttaaaacca agtattattt ttttgtatga aatattttca
    19201 taagaagaaa atacttcttc aatatctgaa cttaaatttt ccggattgta atatgcgctg
    19261 ccaagtatgt gaaaataatc tgaactgata tactgtgaat ctaaaatctt gcctattact
    19321 tcgtgtgcac tttcagcatg tgaatgaatc caattgacag cagtttcaat gtgtccataa
    19381 ttttggatcg aagaaataat tttttctgat aaaagatctg tgtctgagta gtcgagtaaa
    19441 attggattga gtctttcagg atgtcttgtt ttggagagta ttttcttgaa accggattcg
    19501 tgtcttgcta tgacggaaac agcttcatat tcagttaata atttatttac agcaccgctc
    19561 agcattcccg ttccgcctgt gatcagaatg tgagacataa gaattattta ttacaaatat
    19621 tccataagct catcttcggt agtaatgaga acgtcacgtg cattagagcc gtcctttgga
    19681 ccgactatgc ctgcttcttc gagctgatcc actatccttg ctgctcttgc atacccaagt
    19741 ctgagccttc tttgaagagt cgatgctgag cattgctgaa gcttgaaaat aagtttagct
    19801 gcttcgccga ataactcatc tctgtcttct tcacttccgt atgttataga ttttctttct
    19861 ataatagacg gcagcagata tggctttgaa aatccttcct gcgcttctat gaactctgca
    19921 agtttttcac actcttcagt cgttatatat gcattctgaa ttctgatcgg tttggaagat
    19981 gctgatgaca aataaagcat gtccccattt ctcaaaagct gatcagctcc acccatatcc
    20041 aaaatagtcc ttgaatcaat ttttgatgca acctgataag caattcttac cgggaagttt
    20101 gctttgatca cacctgtgat aacatccacc gacggtctct gagtagcaac aatcaaatga
    20161 attccgacag ctcttgccat ctgtgcgatc cttgcaatcg gttcctcgat ctctctcttg
    20221 gctgtgatca taaggtctgc aagctcatcg atgatcacga ctatgtaagg cattttctta
    20281 tgtcttatta cttcattgtc agtaagattt ccatttgcat acttttcatt atatgcttca
    20341 atatttctca cacctgcatt tgcaagtttg tcataccggt tttccatttc gcgttcgaca
    20401 ctttttaata ctgatactgc attagccgga cttgtgataa tactttcatt tatatcacct
    20461 gatgttgcaa ggaaatgttt tttaagtttt gaatataagc tcagctcgat cttcttggga
    20521 tctatcatta caaatttcag atcggacgga tgaagtttat ataagagact tgcgataata
    20581 gtatttatac cgacactttt tcctgaaccg gtcgcacctg cgatcagaag atgcggcatc
    20641 tttgcaaggt cgtctatgaa gacttcgccg tctatagtct ttccaaatgc tacaggtaat
    20701 tgctttttac tatcgttaaa tttttgtgaa acgaatgaac ttctgatcct cacaagttgt
    20761 gctttcttat tcggaatctc aactccgact gtacctttgc cggggatcgg aatgatcatc
    20821 ctgattcctt tggctttcat tgcaagagca atgtcgtctt gtaaagattc gatcttggaa
    20881 agttttacat ctgccgctgg tacgagttca taaagtgtaa ctacaggacc cggcgttgca
    20941 aaaacttttt ccacttcaat gccaaatttc aaaagttttg cctgcagcag tcttccgttt
    21001 tctgtaagct cttcatttgg aattacttca aactcttctt tggtcggctc ctgtaataat
    21061 tctaatgtag ggtatttata attttcaagt ttctcttctt tgaaatcttc ttccttcttg
    21121 ctttgcagat tctgaacgtc gtttgaattt tgcggattca cgtccatttc atttttaatc
    21181 tcttgagcac cagcagcatt gattaacaca ggctgagaaa gctcctgtgt tttctttggc
    21241 ttgatcacta tcgatgaagg atcatcctgc tttggtctgt aaatttcagt ttccttaata
    21301 agctcatttt catttacagt ctttgaatat ttatttttta atattctctc acgctctttc
    21361 ttaagtgcaa tttcaaagtc agcatcatct accggtacat catcaggaat attgtttttg
    21421 attttattac tcttaagttc tttttgtgta acaagatctt ctttctccct tttgaattta
    21481 tcaaagacat tttcaaaaaa taatttaaac cttaccaaag tcttgataat gtttccatca
    21541 acgagaagga atccgagcaa aataattgat attgaaaaga aaactgtaga tccaacacta
    21601 ccgagagtat gataaaatat cgatgcgaaa taatcacctg tcgtcccgct catctttgtt
    21661 gaaatgaact gcggtccggt gaaaattctc aacagtccga aaaatgatgc agtcatcatc
    21721 atcagtaaag atgcgtagat gctgaattgt actgttctgt taagaggttt cttaaataat
    21781 agtgtaatgc cgaatgtgat cagtatgaca gaaatgatca atgagaaata tccgaagaaa
    21841 taatttacaa gaaaatcaga aagatatgcg ccggctattc caagccagtt gtatgtaaca
    21901 aaggctgagg agtaagattc ttttttgaaa atattgagaa agctcatgcg ctcgagagtc
    21961 gcctgatcct tgtctgaata ggacaaaatg gaaagacata tcattattcc gaatacaccg
    22021 ataagaaatc caagcaactg ttttttgaaa tcctcggaaa tagtgatatc ttccttagat
    22081 gaatttcttt ttacaggtgc tgcgcctgtc ttgggtatgc ggggtttttt actttgcttc
    22141 atttctaaaa attaaattag aaaattaatt ttaagccttt ttttctttta cttcttcttt
    22201 cttaactcca atgtttactc ttctcatctt tgacttctga ctagactcac cttcaaacat
    22261 ttccttaaat tttacaactc catttctgaa cagcggaatg aagggatatg aatcaagctt
    22321 tgatgctgtc tcaagatctg ctccaaatcc aatggaagtc agatatttac catgttctga
    22381 aatattcagc atcctcaaga tcttttcctg tgatggaaca ttcagaagca ttgcaagatc
    22441 attacaaaga ttcattgcag cgatctcgga atctccaagt tcaagctctc tgccggcaga
    22501 aagtttattg agtatcaacc ctgcgcagac agtgtcttcg agacaaaatt cattcagctt
    22561 accggcacag ataattataa aatcttcatc aattacattt acataatcca ccaaagccga
    22621 cagatttaaa aaacttccca tcacacagct ttttgcaaat tttgatttga tgatcgcaag
    22681 cgttccgttt gttgtactga atatcagtcc ctgatctttg atcacttcag ttgtgtattc
    22741 agaaggtgaa ttaccaagct tgaatccgtc cacgactttt ccgtttcgct ctccgcaaag
    22801 caatgaattt ttggaacctc tggcaactct tacagccgta gcaatatttt ccgttggaac
    22861 aatttcttta gcaccgtttg ccagagctat tgtcattgtt gtagttgctc tcagcacgtc
    22921 gagtatgata atattcttat ctttcagcat caggtcatcc tgtatcagcg attgcgtgag
    22981 taatatcttt atacttttca ttttattttt ctgtaaacct ttctttgtgg aaatttttat
    23041 tattttaatc ttaactcaat tttataatat caaataacat tcattgtaaa aacggagtct
    23101 ttgttatagt agctttaatt tttttatctc ttactttaat ttctatctga gttcctatct
    23161 tattgaatcc tttatctata tatgccatac cgatattttt attcagcata ggtgaaagac
    23221 ttccgctggt caccattccg atcttttcat cacctgagaa aacttcctgt ccctgtcttg
    23281 caggcagtcg gtcttccact ataaatccta caattttctt ttcgggattt tcctttgcat
    23341 gaaggatcgc ttcttttcca ttgaaatctc ctttatcgac tttcgtgatc catccgagac
    23401 cagcttcaat agtgttgttc gattcattca tatcattgcc gtataaacga aaagcatatt
    23461 caagacgaag agtatctcgc gctccgagtc cggcaggttt cagatcggat tcctttccag
    23521 cttcgaagat cgcattccat aatgcttccg acttcgcgat atctgaagat gaatatattt
    23581 caaaacaaat tttctcaccg gtgtaacctg tatgtgataa tacaacgtcc tgaccggcta
    23641 atttacactc tacgaatttg taatactcta tctgtgaaag atcagcatcg gtaagttttt
    23701 gtaaaatgtg aatagaattt ttcccctgta cagcgagcag tgaagtatcg tctgaaatat
    23761 ttttcagagt aacatcacca aataaattct cttccatcca ttttatatct ttctcaatgt
    23821 tcgatgcatt tatcacaagc atataatgtg aaccaaaatt ataaacaagc agatcatcta
    23881 ctataccgcc gtctttcaga cacatagccg aatactgcac gtcgcctttt tcaagtttgg
    23941 atacatcatt ggtcgtgatc ttttgtaaat aatcaagtgc atcactgcct ctcacttcga
    24001 cctcacccat atgtgaaaca tcaaatattc ccgcggactc acgcactgct ttatgttcgg
    24061 caataatgcc ttcatactga acgggcattt catatcccgc aaactcaact aatttaccgc
    24121 cgagactttt atgaatatta ttaaaagctg tttttttcaa ttaaatattt tctttaataa
    24181 tttatttact tcttatttac cctgaagctt ttcccagtcg ctgagaaatt tatccagtcc
    24241 catatctgta agcggatgtt ttgcaagcat ttctatcact ttaaatggca tagtcgcgat
    24301 ctccgctccg agcagacagc tttcgacaaa atgtatcgga tgtctgaccg atgcgacgag
    24361 tacctgtgtc tgataattat aatttccgta gatctgcact atctgaccga taagctccat
    24421 tccgttttga gaaatgtcat cgagtcttcc gacaaaagga ctgacaatat aagcaccggc
    24481 ttttgcagcg agaactgcct gagacgctga gaagcaaagg gttacgttgg ttctgatatt
    24541 ctcttcgctg aatactttta cagctttcag accttctttt atgagaggta cttttactac
    24601 aatgttttta tggatcttgg caagttctct tccttctttc agcatatcgt cacaattggt
    24661 agagactact tctgcgctga tatctccgtc caccatagcg cagatctttt cgagcattga
    24721 tctgaaatct ttatgtcctt cttttgaaac aagtgacgga tttgtcgtga ctccgtcgag
    24781 aattcccatc gcagcagctt cacggatctc atccagatta gcggtgtcaa taaatatttt
    24841 cattatgaat taaattctta taggattact ttataaaacg tttgtaaagt gtagtaaaat
    24901 agttattata ttgtattata aaaagtgaga atattttgga ggggagcaaa aacgaattaa
    24961 gaattacgaa ttattctgtt acaacgtaaa ttaatcgcaa aatggtaaga aaatttttaa
    25021 ttaatttttt agaaatgaat tcaaaaattc aaacagcacg tttcagaata attaaaaagt
    25081 aattccacct taaaaatgag ggagcgaagg gggttattgc tgaaagaaat agcaaacaac
    25141 aaatataaaa ctcagcactg tgtgcagtgg agtcaaatta aactcaattc ttcaaacccc
    25201 tcccaacctc ccttttggac aaaggggagg agtattaact ttttaaaaat tttttttggt
    25261 tccagctatg tcagtttata aattatgaaa ataaagatat tgattataaa attaagaaga
    25321 ttaaatcagg gaaattatat aaatcgatca ggaattgtca accaatcttt ctttaagagc
    25381 ttttgcgact gcttcggatt ttgagtggac atggagtttg tagtaaatat tttttatatg
    25441 atggcggact gtaccgactg tgatgaaaag attaaatgca atttccttat aggaattgcc
    25501 tttgcaaagc tgatcgagaa cgtcagtttc tctggaggtc agcggtgatt cggttttttt
    25561 ctgaaatgat gtgacgacaa gttttgcgat cttataagac attggcgcgc cgccttcata
    25621 aatttcacgg atggattcaa gtattttttc ttctgtggca cttttatcaa gatagccgct
    25681 tgctccggca ctcaaagctt tgaatacaag tgaaaggtct tcgtgaattg tcagcataat
    25741 tatgtcgatc tttggataaa tttcctttat cttttttacc ccttcaattc ctgaaatgcc
    25801 cggaagctca atatccataa gcataacatc aggtttattt ttcccaatat cctttaatgc
    25861 actttcacag tcaccgtaat ttccgataca ggaaaatcca ccggcttcat taatcatttc
    25921 tacaagcgat tctctgataa gcttatcatc ttctaccact gatacatgaa tcctgaaatt
    25981 ttcagatttt ctattcatag cataattttc aggaatcaga agatttgttt tccgtaaaaa
    26041 ccgagatcag attctgaatt cctgcagtca aagtaatttt cgtacctgct ccgggcattg
    26101 atgaaatatt tacattcaga tcgatctgag cggcacgtga gaaaatattt tttaatccgt
    26161 atcccttttg acaatttgcc ggatcaaagc caataccgtc gtcctctaaa attatttcaa
    26221 catcttcatc atagatcctg aagtttaatt ttacattttc acattcagaa tatttgagaa
    26281 tgttattcat accctcttta aaaatatata taagatgccg cttacaattc atcgaaagcc
    26341 ttatctccct tagctcttca gatatttcac ctgagtgaaa ttccatacca ttgttgtcaa
    26401 aaagttcatt cccaaaatct tttaacctaa cagcaaagtc atacaaagta tcttcctgcg
    26461 gattcattgt ccatatgaga tcctttgctc cgctctgtag agagttagaa gtttccacta
    26521 taaattttaa gtcttcagaa attccatttg taaacgcctg aagtttttta tttactcttc
    26581 tgctgtaaag agaaatccgg gtaagcttat gtccaagctc atcatgataa tcacgtgatg
    26641 cctgctctcg aactttttcg cgttctgact cacggacttt ttccagttca agtaaatgat
    26701 taactttact tctcactctt atattctgaa ttaatataat tgaaaaaatc agcagagata
    26761 aaattattgt atagaaccac catgtcttcc aaaacggtgg tgatatgctg aacttaagct
    26821 cggatacaga ttcatttttt acaccgtcag aattagtact tataactttg aaaatgtaat
    26881 tacccggatc gagatttgta taaactgcat ttctgttttt tccgctttgt atccagtctt
    26941 tgtcaaatcc ttcaagcatg tatgaatact tgatcttatc cggatgagta aaatccgatg
    27001 atgaaaaacc aatattaatg acattatcct tatagcttag agaaacatct ttaagtgcag
    27061 aaatatcttc agtaaatttt gaattctcat tatacctgat aagatctgta aacaaaacct
    27121 gaggcgtaaa gctactatta ataatatcgg caggatttac acttgttaca ccattgacac
    27181 ttccgaaaag aattctgcca tcagaagttt tcatgtaaga tgagaaattg aacatattat
    27241 cagacagacc atcgcttgta ttaaaatttc taaaatcatt attatttata ttgaataagc
    27301 tcagaccatt aattgtactt agccagagat taccgtttac atcctgcaca attcctgcga
    27361 ttactgaatt atcaagacca ttcttttcaa taaaatataa aaaactttcc gtagccggat
    27421 cgaatttatt caatccaccg ccggtaccta tccagaaatt tccggatgca tcttcgcaaa
    27481 aagaatatat atagttgtta ctaattgttt tgtcatttaa aggatcatga ctgaaatgac
    27541 tgaatttgcc agtagctctg tccattttat ttaatcccga ataagtgcca acccatatac
    27601 ttcctgactt gtcttcatat attgaaagta tagaattttc actcagactt tccatatctg
    27661 tcggagaatg taaatatctt ttgaattttt tttctgcagg caaatattca ttcagcccaa
    27721 agtctgttcc cacccaaagt ttacctgcat tgtcagtaag aagtctgctt attccattat
    27781 tactaagaga atgttcatct tcgggtgaat ttaaatattt tgtaaaagtt ttattgcttt
    27841 cattatattt attcagaccg aagcttgtac cgatccaaag atttccgtta atatcttttg
    27901 taagtgatct gacaaaatta ctgctaatgc tattctgatt attcttatca tttaagtatg
    27961 tatctaatat gttaaaatta ttgtcaacct ttaacaaacc ctgagtagtc ccaatccaga
    28021 tagtaccatc cggattctca taaaaacaat gtacactttt attttcaaga tagttttgaa
    28081 acttcccgtt tccgcgaatg accttatgca acccctcctg ataattacta agccatagaa
    28141 ctcccgaatt gtctttatac atccgtgtaa taacttccct gttgaattct ttatccggat
    28201 acagcttcac cttttcgaac tttccttcgc gcacatagaa acggttaaaa ccgtttcccg
    28261 tccccacacc aagcaccccc gcctcttcct ccaataccga aataacaaca ttactactaa
    28321 tactattcac atcccccgag tcaactacat acctcttaaa atctccagtc cccctctcaa
    28381 acttattcag tcctccgcct cccgtacctg cccagatgtc accgtagctg tcgcacagca
    28441 atgaattaat gacattgctt ccaatgctgt tctcattttc cttatcaaat ttatatactg
    28501 tgaactcacc tttctttaca tcaagtttgt tcagaccggt agtcgatcct gcccagatgt
    28561 taccgtcatt gtcaaaactt aaagttaata tcttattacc actgatactt ttggaattag
    28621 tagagtcatg tttaaaagaa gtaaataaat cattttcaaa tttacaaagt ccgcctgatt
    28681 ctgttgcgaa ccagatagtt ccgtttttgt cctgtatgat atcccagatc gtgttatcgc
    28741 taactgtatt ttcattattc ggatcataaa aaaatcttgt gaaatttccc gatctcctgt
    28801 cgtatctgtt cagaccgccg ttatatgtgc caatccagat atatccatcc ttatcttcaa
    28861 aaatggaaat tatgtcgtca tttgaaagtg agtttttatc agaaggatca tgtctgtatg
    28921 ttttgtaatt atccccatca tatctgacga gtccgaacat tgtcccaaac cacataaatc
    28981 ctttgctgtc ctgataaatt tcaaagacaa gattcgttgg aatgccaagg tcatttgata
    29041 tatgttcgat tttaatctga ttctggaaag tgttattttt tatttgattt tcctgagctg
    29101 taatacttcg gaataataat gtaataaaaa ttattacagg tatgatataa atattaattt
    29161 ttttcatacg ataaattaat taatattgga agagcattca atctcatata tatgagatgt
    29221 gatttttatt ataagaattt atatatatta aatttatgca aacttattgt tttaagaata
    29281 ccattaaata ttcaaatatc tcatataaaa tttttaagag aatccgtatt tcaaaaaccc
    29341 accgtctaca gctatacatt cacctgtaat ataagatgca gccggcattg aaagaaaagc
    29401 aactgctgct gctacttcct caggttcgcc gactcttttc atcggagttc ttgccagtac
    29461 tccttcgaga tattcttttt tacttaatac aggttcggtc agagaagtcc ttatatacca
    29521 cggcgcgacg gaatttgccc ggatattatc ctttgcccat tcgactgcaa ggtattttgt
    29581 aaaatgaatt atgccggctt ttgaaactgc ataaggcgat cctgtgccga ctgaagtaaa
    29641 tcctgcaact gatactatat ttacaattga accgctgcct gaatttttta acatcggatg
    29701 aaatatttta caaagttcaa ataccgattt catattaagt gatgatatat aatcaaaatc
    29761 ctcttcagaa ttttcgagag tactctttcg gttatttgat ccggcgttgt ttacaagtat
    29821 atccaaacgg tctgaaattt ctagaatttt cccgttgagt ttttttctgt cacctgcatc
    29881 tgtaacgtca cacttcattc cgtcagcctt atgaccttcc tttgtatatt tttcgagatc
    29941 ggcttttata tcattttcat ttcttgaaac aatgaaaacc tcagcaccaa gtgaaaggaa
    30001 ttcctcagta attgcttttc caattcctct ggtcgcgcct gtgatcagag ctttcttatt
    30061 ctttaaagtc cagcggttat tcataatatg aatttatttt aatagttaat attttttatc
    30121 catcttaagt aatcaaaata ttcaaatgca tttttttcat atttatcttt tttaaatctt
    30181 ataagatcat tagttagttc agacagtaat gttttttgat tcgtattttt attctgtttg
    30241 ctcatttttg aaataaaatt caaaacagta tcctctgtct tatgcaaagc atttcggctc
    30301 ctgagatatt tctttgcaga gggtatcata tattgaagaa gcttgtgatc tttcatctca
    30361 aaatgaatca gtattaaaag cattcgcgca tatggctcaa tgaacggtga caaccccgag
    30421 ttcttttcca gagaatattc accaaagatc cttaaacatt cattataatt tttttcgaaa
    30481 aaatcaatag ctgatagtct gtagactatt agattaaaag tgttaatggt aattttattt
    30541 ttatttcttt ttaaaaattt ttctgtttct gcgagaatcg tgtttttatt tttattaact
    30601 tttccattta aaactcttaa cagattataa tgcagttcgc tcagcataat attcagatca
    30661 tttatttcag tcttacctga gttaatttta agactatcga gatgcttttc agcttttgca
    30721 aactttcgcg atattgatgc ggaatatact gctgacagaa ctgagtcttt gtaactatca
    30781 aacaaagatt catcaaacac atttatatta tttgatatta atttaaatct ttttgtagaa
    30841 taattataag aagcatcaaa atttcctttg atctcctcgg ccatactcat cgcaaataaa
    30901 taacgctcct ttgctgtcac agacataccc attccgtcag atactgtttc tgaaactatg
    30961 agtaaatcat tcatacattc atccagatct ttttcattct gctctgatct taccatacct
    31021 ttagttctgt aaaacctgaa cagtaaggac actgtttttt tataattatt tatatcacga
    31081 atcttgttga gaattttaag ttcatcatca aacacctcct ctatatttac tcctgaaagc
    31141 tcttcctttt ttgtaagctg tctttcaatt tccatgaact caataagaaa gctgaatctt
    31201 tcattctttt cggcaagttg tttacctgct tttacagttt taaagtattg tctgaaaagt
    31261 gctttgtcaa aaagtatccg acatctttgc agcatattaa aaagttttgc atcagtggac
    31321 ttttcatttt tataattcag aagactttta aaaataagtt tggtgagata atttttagta
    31381 aaattaaaat tttttgtaat gtcttccttt ccttttaaag atcttaaagt tttttcatca
    31441 ttagatcttt gagaatctat cagatcaaag agtttaagat atttcttatt tcctttctgc
    31501 acggatgcat ttagagtgaa atatcttttt tcactggagg taagcgattt tatcagatca
    31561 aaaagttctg tattagtatt catagaatta aaaaaatatg gtgaaaaagt atccgtatat
    31621 caaccgataa aagtatcttt gtatctcata ggtatataaa ttaaaatttt agatgttagg
    31681 aaatcatctt ctcaggaatc aaatttaagc tctgattttt ccccgaactt ttcgaatcag
    31741 agaataataa cagtcgtata ttttctataa ttttataaat attaaacttt aaaaaaatga
    31801 aaacattaat aaccattttc ctgatcacat ttacagccct aacacattta aagtctcagg
    31861 actttatcaa aatagaacaa agtattgtaa gtcttgacag cggcgcttca cgcagcgtaa
    31921 actggatcga ttacgatagt gacaacgacc ttgacctctt tatatcaaat ggtaaacagg
    31981 gaggtgaaaa taattttctg tacagaaatg acaacggtac ttttgtaaag attgagaatg
    32041 atcctgtagt tacagacttt cttccttcgg acggtagcag ctggggagat tttgataatg
    32101 acggatttcc ggatatgtgt gtcgtgaact ggtataacag gatcaaccca ctctataaaa
    32161 ataacggtaa cggttctttt acattacttt ctaacagccc tgtctcttta gatccgagtt
    32221 attcagaaac atgcagttgg ggggattatg ataatgacgg tttgttagat ctctttgtaa
    32281 ctaacagcgc aggtaccaat cataaaaact ttatatacaa aaacacaggt ggtggtaatt
    32341 ttataaaaat tgatacaggt gcagttttca gtcagacagc atttgcaaga ggtgtgaact
    32401 gggtggatgt aaatggtgac agacttcctg agatttttgt atccagggaa aataatcaga
    32461 atgagcaatt ctttaaaaat aacggcaacg gatattttac agcaatcaca aatactcctt
    32521 taacatctgg cggtggagaa tcatggagtg caagctgggg tgattatgat aatgatggtg
    32581 atctcgatgc ctttgtaact aatactaaca atcaattgaa ctcactttac agaaatgacg
    32641 gcgattttac ttttacaaaa atcacaaatg atcctttggt tacagagcag ggatataatg
    32701 ctgtaggcgg ctggggtgat tatgataatg acggtgatct tgatatgttc atttctcagg
    32761 catataaagg tcctacattt acagagaagc ttgtgaataa attatacaaa aataatctga
    32821 tggaaacagg taccgcttct tttgaaaaat tgacttctgg agttcttgta aatgattcgg
    32881 gatatacttt cggatttgca tggggtgatt atgataatga cggtgatctt gatttggcag
    32941 cagcaaatac ttttaacgag aatcaaaaaa atgctctgta tagaaatgaa ctttcaactg
    33001 gaaacaaatg gatcaatata aaatgtaccg gtaccacttc aaacaaatct gcgatcggaa
    33061 ctaaggtcag aataaaagct aacatcaatg gaaactctgt gactcaaatg caggagatca
    33121 gcggacaggc aggatactgc actcagaatc tgatacttca cttcggtctc gggaatgctg
    33181 cagtgataga ttctattaag gtcgaatggc cgtcaggtga agatcagtat tttacaaatg
    33241 tacctgtaga tcagaatata actatcactg aagacggaac attaatttcc ataaacgaaa
    33301 atgagataag cgttgtaaaa gattttcaat tatttcaaaa ttatccgaat ccttttaatc
    33361 caaacacccg tatcagctac aggttaaaca aaaattcaaa agtacttctt cagatttttg
    33421 atactctggg aaatgaaatt accacactgg taaatgattc acaaaatgaa ggtgagtatg
    33481 aatcagaatt tacaggtaat aatctaagtt caggagttta tttttataaa cttacagtaa
    33541 atgaaatatc gcagacaaga agtatgatac ttgtaaaata aaaagtgaaa gtgtttttca
    33601 taaattttaa tttttaatca tgtagagagt gtcatgaata gggttggatt tgatggtttc
    33661 tgtttatatt tataaaataa gaccggtatg aaaatgccgg tttttttatg tgaaaaatac
    33721 ttttaaaaaa aatttaatct gaataagttt ttattgtaaa agcccttcat cccaaaaata
    33781 ttcctcactc ctgaccagtt ttacttttct tttgaaatct gaatgctcga tatttattat
    33841 gtaattttgt tcctgaggaa ttactgctga aggtactttt agtaaaaggg tttctttact
    33901 ttggatccaa tcacttccaa tctcctgaag tttagaatag gcttctaatt ttctccagtt
    33961 ttcaggcagg tcttttgtct taactgtctt tataatattt tccgaatcgg ggacagatat
    34021 tatcaggact ttatatatta tatcaggttt gataaaattt cgatgaacaa ttaattcaag
    34081 tgtcgacaat gatctggatc ctcctgtata aattacatgc tctccgtttt tattccatct
    34141 gtttggtctt cctgaagaga caagcccttt cgaatatttt tcagatgata ttctgaataa
    34201 ttccatttaa taaattgtta aacattatca ccaaattcga gagctgtcag tctgtcatca
    34261 atatacctga tacctgtaat ggtcttgaga tattctatcg gtgctttttc attacaacca
    34321 atgcagtcca aagactatag accggtaaaa ataaagccat acttttttct actctgtaaa
    34381 tttcattttt gtctttaatt gttttataaa gtctgaatgt aacaaacaat aaatttataa
    34441 ggataagaat atttccgact aaaactgcaa gcttgttagg ataggtcccc cattcataaa
    34501 ttctgaaaac tatagctgaa agtgcaattc catttacaat catcgtcagt acagaaagtc
    34561 caaacagcat atatacactg attttgattt ccgaaccttt tgaagtttat gcaactcaaa
    34621 aaagtataat tgcctacatg caagtatcag tgtgtgacta ttcatattgt ccggcaataa
    34681 attcatataa aagacagcga ttaatatgat tatgaaagca gggagtaatt ttttgagatt
    34741 tactttttgc ttccatgcaa aatatgatat ccaaaaggaa aaactataag tgataaattt
    34801 ctttgataga aatattcagc atccatacca atcatctccg gcagcttagc caaaaaacct
    34861 gcaagcagcg ataataaaac aacaaatttt aactcgttgg ttttttccca ggaaatttca
    34921 tcgtttttaa aaatga
//
LOCUS       DAORVQ010000010        33609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_398_length_33609_cov_6.346874, whole genome shotgun sequence.
ACCESSION   DAORVQ010000010 DAORVQ010000000
VERSION     DAORVQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 33609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33609
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_398_length_33609_cov_6.346874"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1433
                     /locus_tag="PLX80_01845"
     CDS             153..1433
                     /locus_tag="PLX80_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19569.1"
                     /translation="MQFNKYFFVGISVLVIMFSVNESRSQDTAKIMSYNLLNYSGNNT
                     TKDPYFRQTMDYEKPDVLAVCEILSQAAVNNFLTNVLNFNNPNKYSAGIFIDGPDTDN
                     AIYYDNSKFLFVSNTVIATQLRNINMFTLIHIKSNDTIKIFDVHLKASNTSDDQQRRL
                     AEVNLMRNVTNTFRIGEEFMVVGDFNIYSSNEPAYQRLLQPDVNSEGQFLDMFNLTGT
                     WNSSSYAPYHTQSTRTRSIGDGGATGGLDDRFDMILNSTGLTQEGKIKYIPGSFVSVG
                     NDGNHYNDSINKQPNTAVPDSIANALYYTSDHLPVYAKYKFDDNPISVSNISSEIPEG
                     FKLSQNYPNPFNPNTRIDFDIPDLRPYGSFTGRVTLKIYDILGNEVKTLVDENLSEGE
                     YTSEFNASGLSSGSYFYRLSAGNFTLTRSMTLIK"
     gene            complement(1567..2796)
                     /locus_tag="PLX80_01850"
     CDS             complement(1567..2796)
                     /locus_tag="PLX80_01850"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQY19570.1"
                     /translation="MKKIQFHLKFLSLTFLIMLIVISIYSDSIAQTTVNITYATDNTL
                     YEDPNGETSNGSGQYFFTGQTAADLTRRGLIKFLLVKDIPSCATITNVSLKLHMSKTI
                     SGFEDAELRRVEKDWGAGNSYAPGEEGYGAQAEDYDATWQHNFYDTEFWANDGGDFSS
                     DPSAVEPVGGNGFYYWDSTAMIQDVQNWIDNPESNFGWVLIGNERDLGTAKRFDSREN
                     DSVNFRPVLTVTYEVNAIPLYSVSLIEGFLDGVNMIRDSLKVSLRSNVSPYNIVDSKT
                     KMSDELGGVLNCFNAADGTYYIVVNHRNSIETWSKNPLAFMNGNFEYYDFTSAATQAY
                     GDNQVLKLGSYCIYSGDVNQDYIVDVTDGSIVDNDSFNFASGYIPSDVNGDDLTDLAD
                     GSIVDNNAGNFVTRVSP"
     gene            complement(2869..5469)
                     /locus_tag="PLX80_01855"
     CDS             complement(2869..5469)
                     /locus_tag="PLX80_01855"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19571.1"
                     /translation="MKLSLTIFISVFIFAAICLKLDFPLKSPDTSENKNATTEEDLKY
                     PNENEYVKRTFPFYKADPDAHLVALKQAQEMRSETLSKSQSDNLIPAWEFAGPVNIGG
                     RFSDIEYDPNNTDIAYAGAATGGIFKTMDGGVTWNPVFDDQAVLPIGDLAVDPVNSNI
                     VYAGTGEANGGHNNFAGGGIYKSTNGGDSWQLMGLENTVSIGRIVINPLNPLRVYAAA
                     AGSYFAPNIERGVYRSDNGGNTWSKVLYINDSTGAIDISIDPVNPNNLLAAMWQRTRY
                     PNGGQLYGPSSGVHKSVDGGNTWTVLGPANGLPNSSTSNVGRIGLSTSVTEPNISYAL
                     FTNGTTYSGFFKTTNAGLNWTNANPNGTLQNGFSTFSWYFGNVRVHPADPNTVYVLDF
                     SINKTTNSGNSWSELQSNNIHVDQHALAFKPGDPNRIIFGNDGGLYNSSNGGNSFTKV
                     ASQPVTQFYEIGLDKTNPQRLYGGTQDNGTNRTTTGSTSDWSSIYGGDGFYVIVDHTN
                     PNIIYAESQNGGLGKSTNGGNTFSSATSGIPSGEKRNWSTPVVMDPNVNTVLYYGTNK
                     VYRTTNSASSWTAASPDLTNGNQARLGTVTSIDVAPTNSNIIIAGTDDANVWITTNNG
                     TNWTKVSQTLPYRWVTRVAFDPQNDNIAYVTYNGLKWVDPQPHVFRTSDRGATWQDIS
                     SNLPDAPVNAFAVDPLRPNVLFVGSDVGAFVSFNTGQEWQYLGTGMPMVSVYDFKIHP
                     TAYYLVAGTHGRSMYKIDLTQVVGISSLSQNIPDNFNLEQNYPNPFNPLTTINYNISG
                     SGNVSLNIFDITGRLVRNLVSTIQNTGSYTSVWNAMDNNGSAVSSGIYFYSLSFDGNI
                     SETKKMLLVR"
     gene            complement(5776..8244)
                     /locus_tag="PLX80_01860"
     CDS             complement(5776..8244)
                     /locus_tag="PLX80_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19572.1"
                     /translation="MKKITLFYIIPLFVISIYLSGFNILGNNDDPNLDQPPKQYWDHV
                     SGSMPGEVTVITDNSGFDNFDLGVDFSESHISSSPKNPNWYFVAYNTNATYYTLNGLD
                     WQRNNPFFPNQAGDPVTAYDSLGNLYYDNMKSPITGTWNIKSTNGGVSWLSPVSANIG
                     NDKNWIAADQTNGPYSNYIYGVMTPGNVVRSTDGGASYNLVFSASNNLPGMMVAVGAN
                     TVGGNVSGGCVYVVTNTGNSFAATYSFFRSTNGGQSFDFMSSQNFANYVGTAVSGRNS
                     VQNFRTRPYPFITADNSFGPNRGRLYLVYSSNTPAGDGNKPDIFCRYSTNHGTTWSSE
                     VRINDDPNTVLHNQWHPSTWCDKETGRLYVQWNDTRDTPTSDSALVYASYSTDGGATF
                     VQNKMISNKKFRINCTTCGGGGTPAYLGDYSSITSNSVTAMLTWTDFRNNNFGSYVGY
                     YPDFALKTSSQSISVNSGQNVTFRAIVPAVKDYGRVVKFTATMDTLPAAGSLNISFVG
                     KDSVNSFPDSVSINVAAVGSVTPGRYSIKVIGKGPNGTPIHSRYVSVLVNSSFLTVGT
                     NRPGTAEFSVNGIVYNQTQQLVFTNGASVNVTAISPRVVGATQYVFTNWSNSGDTSQT
                     IVINSNTNLTAFYKTQYRLLITSSVGNTFGGGVYYDSASSPQIGVLSRVINSGGTNYY
                     FRGWTGIGAGSYTSPDSTGLDSVITINNITVPIVETARWTTTVGINQLSSLVPDKFSL
                     YQNYPNPFNPTTNIKFDIIKSGGVKVIVYNALGKEVNVLLNENLSPGTYKVDFDGAGF
                     ASGIYYYKIVTSEFTDVKKMLLIK"
     gene            complement(8659..10932)
                     /locus_tag="PLX80_01865"
     CDS             complement(8659..10932)
                     /locus_tag="PLX80_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19573.1"
                     /translation="MKNYFRFAKVLFVTCLLIVCAMFSNNLHAQINVGGQPMSSMTEL
                     SDVFSSVTMPDVDVQRLIEEDQITEKEPGMPFRYGYNFDVNYNLNNSGTWETLSDGSR
                     VWRLAVRSEDAVSINLAFKEFHMPKGATFFVYNVGKNTIYGAFTEQNNSEDGYFATTP
                     TYGSTTILEYYEPVYSKNKGRITLHKVTHAYKDIFGYNSVLEEPCNININCPIGSPWV
                     EQKRSVTRITFSQGGGSFLCSGALMNNTLQDRQPYYLYAEHCATDNFSTIVFYYNYET
                     PTCIGTLGSLANTMTGATLKAANFDTDFRLVLLNQSVPASFNAYYNGWDRSNTQPLNE
                     TAIHHPDGAVKKISVDNQPANTVTGFGGRLTNGFWLVVWDEGMTEGGSSGCPIYDQNK
                     RVIGQNLGGTPANCANPQSVQKYFGKLSESWAHGGTPATQLKEWLDPNNSGVMTIDGI
                     DAVTGVAPVSNFTSNIQTLPITGGTVNFFDLSTNGPTTWDWSFPGATPSTSTAQNPGN
                     INFPTTGNYTVSLTTSNAFGANMKTIVAYIKVQGVPISPITLQSPVNLSRIVVSPNDP
                     NITQFKWTSSSPSNTVVYRFKLKKVATPTEDIFASDNNGLDTVMSLRNSFLDSLAASY
                     GTTGDSVIMTWRATAFNGADSVTSNPFIITVRRMPVGINQISSVLPEKFNLYNNYPNP
                     FNPSTNIKFDIVKNERVVLSIYNMLGEKINTLIDQNLSPGSYNVDFNAASLSSGMYFY
                     RLETAGFSETKRMALIK"
     gene            complement(11009..13096)
                     /gene="glgP"
                     /locus_tag="PLX80_01870"
     CDS             complement(11009..13096)
                     /gene="glgP"
                     /locus_tag="PLX80_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQY19574.1"
                     /translation="MENLILKLKEIAYNLSWSWSKDTVELFNDINPDFWNWSQHNPVM
                     VLNEINPSYLEFILEKKNLGSRINDIYADYKNYMSKSTYFDEKYFKTEDPIIAYFSAE
                     FGITECLKTYSGGLGVLSGDHMKSSSDLGIPLAGIGLAYNYGYFTQLINQNGWQTENY
                     ELNDFKNLPIYLLRDENYIPLKVAVNFPGRAIYFQVWVAKIGRVKLYLLDTFIPENSV
                     KDRRVTDILYGGDNESRIQQEILLGIGGFRLLKKLGFDIKAYHLNEGHSAFLCFERIR
                     DKMKSENISYDEAKAKCYGSNIFTTHTPVPAGIDIFTKDLFMKYFTEYAEKEVGVSPE
                     KLFSEGDLNKGQADNDKFNMAFLAINNSNLLNGVSKLHGEVSRDMWKLPKTRSQIDHI
                     TNGVHIASYVSIHTNKLFTGYFTKDWKYDENIWSRIDEIPDEEIWALRLLNKYQLINF
                     CRERITEKWISMGADKEKLKDAELILNRDALTIGFARRFATYKRGNLIFSDLERLEKI
                     VNNSKKPVQFVFSGKAHPKDEGGKNLISQIVAFTKDEDLKNKVLFLDNYDINIARHLV
                     EGCDLWLNNPRRPKEASGTSGMKVIANGGLNFSILDGWWDEAYNQGNGWKIESLADEN
                     IPLEQRDLFESMSLYDTLEKQIIPTFYKRNSKGIPAEWVKMIKNSIRELAGTFNTNRM
                     VKDYCEKFYLNVK"
     gene            complement(13245..13433)
                     /locus_tag="PLX80_01875"
     CDS             complement(13245..13433)
                     /locus_tag="PLX80_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19575.1"
                     /translation="MGSQILFFLLFITNLSFAQIYENRVIRYNGTGNNRDEVIAIDHF
                     YKLLAGSYIDQKQMLLIK"
     gene            13871..15712
                     /locus_tag="PLX80_01880"
     CDS             13871..15712
                     /locus_tag="PLX80_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19576.1"
                     /translation="MRKIANYDLGFHLAGGRWIIENMAFPVKDMFTYTVSANDYLDIQ
                     WLYQVVMFSVQSFTGDHGLTIFNAAAITFVFFLMLKRNNVNDVYIPVSLIMILVTLIC
                     IQVRFSYRPEIFTWIFMLLMLIVLDRNFLYKDKIKKTGLYFLPLIMLFWVNMHGLFII
                     GFVIMISYIISGFVRDKKFDKDLLIWTGISFAAVFINPYFSEGVLFPFYLFTRLQKGN
                     IFQINILELQSPWGMTDILPTELYIYYSVSVLAFILLFITLNHRKFHEFALMSAFFYL
                     SFSAFRNIPLFMIYAFFIISISVSDLIREKNIFSKGNSVTKFSKVLSIIIITVSILTG
                     LRVITDAYYIPYGSEIKFGTGVDKLALPDKASDFINENTPDGKIFNNLESGGWLEWKT
                     GRKVYIDGRLEVIREDFYKEYLSTINNAALNSELKKYTPELIVNSILNFNWTNQLSQI
                     REYSLSYWDGISAVYKLQEPSDTLSAFSFKSGLINAGIDTLGFSETEKDRLLRTVRIK
                     NLENWISGFYSFRKNPYYLIPMGNFAFESGEFQTAELLYLNYIKEGEGFINENLFKDI
                     FLNLGSYYYNSNDMARAQYCLERYLEENPGEREIIKKLNEIKKSRKN"
     gene            15684..16367
                     /locus_tag="PLX80_01885"
     CDS             15684..16367
                     /locus_tag="PLX80_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY19577.1"
                     /translation="MKLKRVGKINLKLSVIIPYFNEEKTIEILIRKVIAGDLAHEIIC
                     SDDGSTDSSNMLVFNLKNEFPDLIKISGSSINKGKGSAVRKGLAISTGDIILIQDADL
                     EYDPSQYSELLRPFEDDNVKIVYGSRNLNGNPHSSFSFYLGGVILSKIVNILYDSKIT
                     DESTSFDLECDGFEFCPEVTSKALKRSFKITEVPIVYTPRSKSEGKKIKWIDGVIAVY
                     TLLKFRFRK"
     gene            complement(16373..17530)
                     /locus_tag="PLX80_01890"
     CDS             complement(16373..17530)
                     /locus_tag="PLX80_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQY19578.1"
                     /translation="MKFSAHTTFIFIYLFILFLKSGVSFSQEMETSQPTEGTETFLNG
                     KWELTMIDNEAVTASSFTYGLPFIEINIKERMVNGNTGCNTFSGKVTSEGEKIKFENI
                     LATEMACQYQKDGEFFDALQNCNNYFIDNGILSLKSDEAVRAVFQKIEGAGSIIRDSV
                     KQIMFDKGIGFYASGHEPPWTVEFTGAESLLFNFNTGIEVINFKVIETINDLRDDSIT
                     ILAEGDGDDIIVRILKRECTDLMSGKKFPYEIKLNFDDMLYVGCGDFTTERDSSGTYR
                     INDIWVLEKLGDEELNKSDFKTGMPMIEINIKENSFMGNTGCNKMNGSMKNIGSLIFF
                     SKIASTRMMCPGGYDSRIFSALKESDNFEIENMKLYLKSGDKVLAVWQKID"
     gene            17686..18669
                     /locus_tag="PLX80_01895"
     CDS             17686..18669
                     /locus_tag="PLX80_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HQY19579.1"
                     /translation="MKNLILILTIILISMNAFSQNIYSDKLAHTYSIVARDSVTGEMG
                     VAVQSHWFSVGSIVSWGEAGVGVVATQSFVNPSFGIKGLELLKSGKSPQEAVDELIAA
                     DDGRDVRQLAILDVKGRSASYTGKNCIPEAGSIAKDNYSVQANMMLTDKVWGAMSEAF
                     ENSAGPLAERMIAALEAAQNAGGDIRGQQSACLLIVKGKATGNPWEDKFIDLRVDDNT
                     EAVKEIKRLLKVYRAYEHMNKGDLAIEKNDMDLAMNEYSTAEEMFPENLEMKFWHAVT
                     LANTGKVNESLPMFTEVFLKDNNWRILFGRLKPSGLLKISDSDVQTILDLK"
     gene            18764..19264
                     /locus_tag="PLX80_01900"
     CDS             18764..19264
                     /locus_tag="PLX80_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19580.1"
                     /translation="MSELLILTGACGVGKSTIAKEWARRKNGAIIECDYFTEWIYDKN
                     FIRFSYEEELLTARLSINTAKEYLKLSMPVAIENVWTPVGLEFLKEELSDLEYITKQK
                     FIWLKCESTENHRRDELRVPGDVMGERVDIVNDQLNGYVWPAYLHIIDTTFLTVKETL
                     EQIAKS"
     gene            19382..19882
                     /locus_tag="PLX80_01905"
     CDS             19382..19882
                     /locus_tag="PLX80_01905"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19581.1"
                     /translation="MKLIDALLAEFQYESQLTRNMLDRLPDGKFDYKPHEKSMTLGQL
                     ANHIGDIPNYVGGTLDVDELDFSNSDYKIPTNATKDELVKAFDESVAKAIESFKRHDD
                     DELMKEWTMKDGDKVYMKMPKAQVIRGFVLNHNVHHRGQLQVYLRMNDIPLPSVYGPT
                     ADEQAM"
     gene            complement(19929..>21050)
                     /locus_tag="PLX80_01910"
     CDS             complement(19929..>21050)
                     /locus_tag="PLX80_01910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY19582.1"
                     /translation="LLSFWEKNSFTNYDHIIIGSGILGLSVACEIKENFPERSVLIIE
                     KGIFPEGASTKNAGFACFGSFTEILSDFEYTGVDKTVELISNRWEGIGILRKRLGDEN
                     IGFLNYGGYEIIDEKHSEKLNKIPFVNVKLKDIFNDDVYFIKNERIKEFGFNPSKVSS
                     LIYSPFESQLDTGKMMKSLIRYSVSLGVEIINGCNADSYSEKENYVKLFVNSGDVKDN
                     IVFNCKSLTVCANAFTQKFLPSLKIRPGRGQVVVTKPVKDLKFNGVFHFDEGFYYFRN
                     FGDRVIFGGGRNLDFENEETTEFDVSEKIISDLKKKLKNTILFEQDFEVDYSWSGIMG
                     FTENKLPVIEEISDRVKCIVSCNGMGIALSSFTAKLVIK"
     assembly_gap    21051..21150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21151..22589)
                     /locus_tag="PLX80_01915"
     CDS             complement(<21151..22589)
                     /locus_tag="PLX80_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQY19583.1"
                     /translation="MDKLKNYIDGILTTPYSNNYINNYDPSTGEVYTMLPDSDAEDVE
                     LAVQSAEKAFPKWSALSADRRADHLYKIAALIERDLEKFAYAESKDNGKPLQLALSVD
                     IPRAIKNFEFFASAITQFSSESHHTNSEILNYTLRQPFGIAGAISPWNLPLYLFTWKI
                     APALAAGNCVIAKPSEVTPLTAFMLSEICIEAGLPQGVLNIIHGYGHKAGSAIVSNPG
                     IKAITFTGGTKTGMEISRIAAPMLKKLSLELGGKNPVLIFKDCSYDEMLETTVRSSFT
                     NQGEICLCGSRIFIEKEIYEKFKTDFVKKVRELNVGDPLLEDTNVGAIVSEQHYNKIL
                     YYIDLAKKEGGNILCGGESVELKGRCNNGWFIAPTVIEGLDYKCSPNQEEIFGPVVTI
                     MPFESEDEVLNMANSTEYGLATVVWTSDLSRAHRISAKIQSGIVWINCWMVRDLRTPF
                     GGVKNSGVGREGGLEALRFFTETKNVCIK"
     gene            complement(22602..22904)
                     /locus_tag="PLX80_01920"
     CDS             complement(22602..22904)
                     /locus_tag="PLX80_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HQY19584.1"
                     /translation="MILYNVTVCTEDSVHDEWLSWMMDKHIPDVMETGLFTEYKIYRI
                     LTDSPEEKTYSIQYFLRSMEDYEKYRDKFAPALQADFQNKYKDKFTTFRTILEQVE"
     gene            complement(23358..24146)
                     /locus_tag="PLX80_01925"
     CDS             complement(23358..24146)
                     /locus_tag="PLX80_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY19585.1"
                     /translation="MNIDLKNKKAIVCGSTQGIGKAIAIELASSGASVILISRNEDSL
                     REVLRELPAAKDQNHNFIVADFSDPDSLKEKMHNFISESSPVHILVNNTGGPKGGEAI
                     AADTSEYLQAFSNHLICNQILVQAVVEGMKKEGYGRIVNIISTSVKQPLKNLGVSNTI
                     RGAVASWAKTLAGELGPFGITVNNVLPGATKTVRLKSIIENKSKISGKTIEEVTKEML
                     KEIPVGKFAEPEELAYAVTFLASSLAGSINGINLPVDGGRTGCL"
     gene            complement(24160..25263)
                     /locus_tag="PLX80_01930"
     CDS             complement(24160..25263)
                     /locus_tag="PLX80_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HQY19586.1"
                     /translation="MKKPFPPVYYADYLKLDSLLTSQKPKSDEYGNPAHEEMLFIIVH
                     QAYELWFKQILHELNSISEMFRKDDIDERNIGVAVSRLRRVTVIQKILIDQLGVVETI
                     TPLDFLEFRDYLIPASGFQSFQFRLIENTLGLNIDNRVKFNDKNYYTMVSDEHQKMLI
                     DSQSKDSIYESVDKWLSRIPFLNIDDFDFWKYYKDAVGVTLENEKNIILNNPSLTEER
                     KVKQLEMFESTKENFEALLDEKKYEELRSQGHRRLSYRSMHAALFINLYRDEPILHLP
                     YRLLTYLVDIDEHFTTWRYKHALMVHRIIGAKVGTGGSSGHQYLLQTAEKHKVFKDLF
                     DLPTYMIPRSDLPELPEDLKTKLGFYYTATLNK"
     gene            25371..25964
                     /gene="thpR"
                     /locus_tag="PLX80_01935"
     CDS             25371..25964
                     /gene="thpR"
                     /locus_tag="PLX80_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQY19587.1"
                     /translation="MRTFISLNIDISTKNKIKEFKEQIIENIMSSDPEILNYLKCEDE
                     KNYHITIFFIGDTNNVMLNEIESALTEIISDSIPLQIKMKTGKLNAFPNFRYPRVIIL
                     DVIDPSNGLKNLSKEINKCMLKFGFKSDKPFHPHITLARVRRDRKINLTSLGNILLTQ
                     IEFTTDHFSLMESRLNSSGAEHFSFTRFQYSESLNLY"
     gene            26288..26485
                     /locus_tag="PLX80_01940"
     CDS             26288..26485
                     /locus_tag="PLX80_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19588.1"
                     /translation="MQIQYQVYLTDDIYSNTENLESLSMEEYFKIYVVNNMKDKTYGT
                     SELKQTGEIMGKNFIADNWKT"
     gene            26473..26613
                     /locus_tag="PLX80_01945"
     CDS             26473..26613
                     /locus_tag="PLX80_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19589.1"
                     /translation="MENLKYQIEQKSDNLTIGVPVIFESHIHADNARSHLLITKVSND
                     VT"
     gene            26904..26976
                     /locus_tag="PLX80_01950"
     tRNA            26904..26976
                     /locus_tag="PLX80_01950"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26937..26939,aa:Phe,seq:gaa)
     gene            27360..29447
                     /locus_tag="PLX80_01955"
     CDS             27360..29447
                     /locus_tag="PLX80_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19590.1"
                     /translation="MKTLILNIALILALTFSQAYSQPRAKIFNKATNPEMLLSIPALV
                     PDSCVASGLVAVGNGQYVYLSVFNFGDNTAITSESWTFISKPAGSNAQLISIPSLQWY
                     KFRTDEMGTYEIKATINSSSGTADTTIRIFSTKFTGVGNFGGVPALYPNCMVCHSGNP
                     DFADIFDRWKVSGHANIFKQQIDSGSANYNTNCMKCHTTGYDHNLVNENNGFDDVARR
                     VGWEWQGPPAPGKWDSLRTHFSELAAFSSVGCESCHGAGFSHASNGGDTNRIDINYKA
                     DNCGNCHGEPWRHSIFQQWENSLHSDPVFEGRNVSASQRNTLSDCNRCHDGESYVDFT
                     KNRIGTLNLTTADQEYVACQTCHDPHGNSNEYDLRNLSAGADTLANGTSYSTAGNGNV
                     CMTCHQARRNNQVYVTTGGNVNSTWGPHHSTQADVLFGSNAATFGFPYITGSHKNIEN
                     TCATCHMSALTDTGTVTRDKVGGHSMNLHFESTDYDHTAGCVSCHPGVTSFRDFIAPS
                     DFDGNGIIAPWEDEVEGCLTNLRISLPPTGIDSVSTALITADSSNLNLRKAYWNYLLV
                     TEDQSSGYHNPFFTIQVLLTSRNYTVGIAQSGTEVPEVYELSQNYPNPFNPSTKIDFA
                     IPKTGKVSIIIYDITGKEIRALVNDETMFAGKYTVDWNSLNNSGQQASTGVYFYRFIS
                     DNFVTTKKMMLIK"
     gene            29601..30557
                     /gene="menA"
                     /locus_tag="PLX80_01960"
     CDS             29601..30557
                     /gene="menA"
                     /locus_tag="PLX80_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HQY19591.1"
                     /translation="MTETIQKPAGLKLWLMAARAYSFPASIIPVLLGSVLAVLLNPGL
                     HFNILNFGLTLVGCILVQVGTNLINDIFDFDKGIDKEDKELGIPHGGSMVISLGFVTI
                     KQMKTAAFFSLLISFLIGVYLFTQAGMWILYLSIFGLLSAVFYTAKPLALKYKALGDL
                     QVIISFGVGMTLGSYIVQTGEFSIMPVIISIPLGLLIDAILHSNNIRDINFDGKFGVK
                     TLPILIGEKLSVKFYYFLILGAYLAVLLFVVLNILPWFALLSFITLPLALKLVKMSEG
                     FPSDTQARFEYGTKHIMMTAQLNMMFGLTLVIGLLVSYLFFT"
     gene            complement(30593..31525)
                     /locus_tag="PLX80_01965"
     CDS             complement(30593..31525)
                     /locus_tag="PLX80_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HQY19592.1"
                     /translation="MNTGGYKYENFKNKICPYCQSRLKKGADITVCSECGTPHHKECW
                     DENNGCTSYGCQNNPLSERTERTERSENSDPEDVGSLTVESIRHSLNTIPQEDLIQCT
                     SCSELIESSSVFCKYCGYNLKENEAPEVKSAASDDFKKEYKKRYREKITFARRRQIIT
                     YSSILLLILAIGLLVYISINRLNEYFASDKYRIQILMENYELAIENRNSDSLKIYLTD
                     DYEFYTKSGKRSGKNERIRKFESIFKNTNILEYNIRDLKVINDTSVTANDKKIKFRDE
                     LITDKLNDSEEKIMRIYKSDSTGNEWKIYREITE"
     gene            complement(31556..32716)
                     /locus_tag="PLX80_01970"
     CDS             complement(31556..32716)
                     /locus_tag="PLX80_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQY19593.1"
                     /translation="MEILDDRYSRLELITWWDQDILKNAKVLVAGCGALGNEIVKNLT
                     MLGVGNISVADMDNVEKSNLTRSVLFRFEDEGKPKAEVICRHAREINDSVNIKYFNGN
                     IFNLGLGVFKNFDIIIGGLDNREARLFINQSCWKVNRPWIDGAIEVLTGVARIFIPPF
                     SACYECTMSEIDYKIINKRKSCMMLGIDEIIQGKIPTTPTIASVIGGIQVQEAIKYLH
                     KREDLLLLSGKGFVFNGGNNDSYVVEYQRNEDCPSHYVFENIIHIDKKFSSAGISDLY
                     ESGRKLFGDDNFQIEFNNEIVYELTDENTDTTYEFFGNVNLMSVKDVKKEDGSLLKFS
                     SFNSLNTGSELFKRLSDKTFEDMKLPFNDIIILRKGEKEFYIEFEYEDVFSE"
     gene            complement(32888..33157)
                     /locus_tag="PLX80_01975"
     CDS             complement(32888..33157)
                     /locus_tag="PLX80_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HQY19594.1"
                     /translation="MARINITIVDATGNKEQQATLPDDASVKRIMEVLLPKMKMPLTG
                     PDGEPLSYKFHHKASGKQLTESQTLLEAGVKEGDVLRLHPEITAG"
BASE COUNT        10451 a         6205 c         5623 g        11230 t
ORIGIN      
        1 caatattaca gaaaatacag tctgagagct tcataggtta aaaaattttt aacaaatata
       61 cttatttttg aaaataaatt cagatgtata taatattaaa ctttaattta cttttacaag
      121 tctgtatatt acatcaaata aaaatcaata aaatgcagtt taataaatac tttttcgtag
      181 ggattagtgt attggtaata atgttttcag tcaatgaatc ccgttcacag gacacagcga
      241 aaataatgtc atataatctt ttgaattaca gcggcaacaa tactacaaag gatccttact
      301 tcaggcaaac aatggattat gaaaaaccgg atgtactggc agtctgcgag atactttctc
      361 aggcagcagt aaataatttt ttaaccaatg tactgaattt taataatcct aataaatatt
      421 ctgcaggaat ttttattgac ggacctgata ctgataatgc tatatattat gacaattcaa
      481 aattcctttt tgttagtaat acagtaatag caactcagct tagaaatatc aatatgttca
      541 ctctgattca tataaaatcc aatgacacaa taaaaatatt tgatgtccat ctgaaagcca
      601 gtaatacaag tgatgatcag cagagaagac ttgcagaagt caatctgatg aggaatgtta
      661 caaacacttt cagaattgga gaagagttta tggtggttgg agattttaat atttactctt
      721 caaatgaacc agcttatcag agacttttac aaccggacgt gaattctgaa ggacagtttt
      781 tagatatgtt caatttaacc ggaacatgga attcatcttc atatgcccct tatcatactc
      841 agtcaacccg tactcgttcg atcggtgacg gcggagcaac aggcggactt gatgacagat
      901 ttgatatgat acttaattct acaggactta cacaagaagg aaaaattaaa tatataccgg
      961 gttcatttgt ctctgttgga aatgacggta atcattataa tgacagtata aataagcagc
     1021 caaatacagc agtaccggac agcatagcaa atgcattata ctatacttca gatcatctgc
     1081 ctgtgtatgc aaaatataag tttgatgaca atccgatttc agtaagtaat atcagcagtg
     1141 aaattccgga aggatttaaa cttagccaaa attatccaaa tcctttcaat ccaaatacga
     1201 gaatagattt tgatataccg gaccttagac cttacggatc atttaccggc agagtaacat
     1261 taaagatcta tgatatactc ggaaatgaag ttaaaacttt agttgatgaa aatctttcgg
     1321 aaggtgaata tacttcagag ttcaatgctt caggtttatc cagcggttca tatttttaca
     1381 gactcagcgc aggtaatttc actttaacga gatcgatgac tttgataaaa taagaatcaa
     1441 tataatattc agtataaatt tttacaaaaa gaagacgcta aaacaggcgt cttttttttg
     1501 taaaaaagcc cctttatccg gggcttataa tttaatatga gatttcttta gagtcaaaat
     1561 gaaaatttaa ggtgagacac gcgttacaaa gttgcctgca ttattatcta caatactgcc
     1621 atcggctaag tcagtaagat catcaccatt gacatctgat ggaatatatc ctgatgcaaa
     1681 attgaaagaa tcattatcca ctatacttcc gtcggtaaca tcaactatat aatcctgatt
     1741 tacatcaccg ctgtagatac aataactacc caattttaaa acctgattat cgccgtatgc
     1801 ctgtgtggct gctgaagtaa aatcataata ttcaaaattt ccattcataa atgctaatgg
     1861 attcttactc caggtctcta tagaatttct gtgattgact acaatgtaat atgtcccgtc
     1921 agctgcatta aaacaattta aaacaccgcc taactcatcg gacattttgg ttttcgaatc
     1981 tacaatgtta taaggagaaa cattacttct taaagaaact tttagagaat cccgaatcat
     2041 attaactcca tctaaaaatc cttcaataag tgatacagaa tacagcggaa ttgcattaac
     2101 ttcataagta acagtaagaa caggtctgaa attaacagaa tcattttctc ttgagtcaaa
     2161 tcgtttcgca gttccaagat ctctttcatt acctataagt acccagccaa aatttgattc
     2221 cggattatca atccagttct gaacatcctg aatcattgca gttgaatccc aataataaaa
     2281 tccatttcct ccaacaggtt ctacagcact tgggtcactt gaaaaatcac ctccatcatt
     2341 tgcccaaaat tctgtatcgt aaaaattatg ctgccatgtt gcatcgtaat cttcagcctg
     2401 tgcaccatat ccttcttcgc cgggagcata agaatttcct gcaccccagt ccttttctac
     2461 tcttctgagt tctgcatctt caaaaccaga aatagttttt gacatatgaa gtttcagaga
     2521 tacattagtt atagtagcac aagacggtat gtcctttact aatagaaatt ttattaaacc
     2581 tcttcttgtc agatcagctg ccgtttgacc tgtaaaaaaa tattgtcctg aaccattact
     2641 tgtttcaccg tttggatctt catagagagt attatcagta gcatatgtaa tatttacagt
     2701 agtctgagct attgaatcag aataaataga aataactatt aacattatta aaaatgttaa
     2761 tgaaagaaat tttagatgga attgtatttt tttcatgatt ttgatttagt tagtttaggg
     2821 tttgcaaata tacttaccta atatttttat tatagtacaa atttttatct accttactaa
     2881 aagcattttc tttgtttccg aaatatttcc gtcaaaactc aggctgtaaa aatatatacc
     2941 gcttgatact gcagaaccgt tattatccat tgcgttccag actgaagtat aactgcctgt
     3001 attctgtata gtggaaacaa gatttctcac aagcctacct gtgatgtcaa aaatattgag
     3061 actgacattt cctgatcctg aaatattata atttatcgta gtgagaggat tgaaaggatt
     3121 aggataattt tgttcgaggt taaaattatc aggaatattt tgacttaaag aacttattcc
     3181 aaccacttgt gtaagatcga tcttatacat tgacctgccg tgagttccgg ctacaagata
     3241 ataagcagtt ggatgaattt taaaatcata gactgaaacc atcggcattc ctgtaccgag
     3301 atactgccat tcctgaccgg tattaaaact tacaaaagct cctacatcgg atccaacgaa
     3361 taatacattt ggtctaagcg gatctactgc aaaagcattt accggagcat ccggcaaatt
     3421 actgcttatg tcctgccagg tggcaccccg gtctgatgtc ctgaatacat gcggctgtgg
     3481 atctacccac ttcagtccgt tataagttac ataagcgata ttgtcattct gaggatcgaa
     3541 tgcaactctc gtcacccatc tgtaaggaag tgtctgtgat acctttgtcc agttagtacc
     3601 gttgtttgtt gtaatccaga cgtttgcatc atctgttcct gcgattataa tatttgaatt
     3661 tgttggtgct acatcaatag aagttacagt tccaagtctt gcctgatttc cgttagttaa
     3721 gtcgggactt gctgctgtcc atgaagatgc agaattagtg gtcctgtaaa ctttatttgt
     3781 cccgtaatac aaaacagtat ttacattcgg atccatcaca acaggcgttg accagttcct
     3841 tttttcaccg gaaggaattc ctgatgtcgc agatgaaaaa gtatttcccc cattggttga
     3901 ttttccaaga ccgccgtttt gagattcggc ataaataata tttggattgg tgtgatctac
     3961 aatcacataa aatccgtcac caccgtaaat gctggaccag tcagaagtac tgcccgtagt
     4021 agttcggttt gtgccgttat cctgtgtacc gccgtaaagt ctctgagggt ttgtcttatc
     4081 aagaccgatc tcatagaatt gtgtgacggg ctgagatgca acttttgtaa atgaatttcc
     4141 gccgtttgat gaattatata aacctccgtc attcccgaaa attattctgt tcggatcgcc
     4201 cggtttgaaa gcaagggcat gctgatcgac gtgaatatta tttgactgca gttcactcca
     4261 ggagtttccc gagttagttg ttttgtttat agaaaaatcc agtacataaa ctgtgttcgg
     4321 atctgccgga tgaactctta catttccaaa ataccagcta aatgtagaga agccgttttg
     4381 cagagttccg tttggatttg cgtttgtcca gtttagtccg gcatttgtag tcttaaaaaa
     4441 tcctgaataa gtagttccgt ttgtaaataa tgcataagat atatttggtt ctgtaactga
     4501 agtactgagt ccgatacgtc cgacattaga agtagaagaa ttcggaagtc cgtttgccgg
     4561 tcctaatact gtccaggtat ttccgccatc gactgattta tgaactccgc tcgaaggtcc
     4621 gtaaagctgt cccccgtttg gatatcttgt cctttgccac attgcagcaa gtaagttgtt
     4681 cggatttacg ggatcaattg aaatatctat agcacctgtt gaatcattta tatataatac
     4741 tttactccat gtatttccgc cgttatcact tctgtaaaca cctctttcga tattcggagc
     4801 aaagtatgaa ccggctgctg ctgcataaac tctcagcgga ttcaatggat tgataactat
     4861 ccttccgatt gagactgtat tttccaagcc cataagctgc catgaatctc cgccgttagt
     4921 ggatttataa attccgcctc cggcaaaatt attatgaccg ccgtttgcct cgcctgttcc
     4981 tgcatagact atattagaat ttacaggatc gacagcaaga tctccgatcg ggagaactgc
     5041 ctgatcgtca aacacaggat tccatgtaac tccgccgtcc atagttttaa aaattccgcc
     5101 ggtcgcagca cctgcataag ctatgtctgt attgtttgga tcatattcga tatcggaaaa
     5161 ccttcctccg atatttaccg gtccggcaaa ttcccatgca gggataagat tatcggattg
     5221 agactttgac agagtttccg atctcatctc ctgtgcctgc tttaatgcaa caagatgtgc
     5281 atcaggatcg gctttataga atggaaaagt tcttttaaca tattcatttt cgtttggata
     5341 ttttaggtct tcttcagtgg ttgcattttt attttcagaa gtatcaggtg attttaatgg
     5401 gaaatctaat ttgagacaaa tagctgcaaa tataaaaaca gatataaaga ttgttaatga
     5461 tagtttcatt agttcaataa atagggttga taaaaaagat tatgtatttt agctcttatg
     5521 gtttaaagat tcaatgaatt agataaataa ttatggattt actttacatc agcctaaaac
     5581 atttaagtat ttgacaacat ccacgtgaca gtttcgaaag ttatttgaca cacacacact
     5641 cttttcgacg ctccaattca gtccagcact tttgaaaaga agtagtaaag aaattaaaat
     5701 tagaatcccg cagagaaatc atcattctct gcgggaaaaa attcacttca aaaattatta
     5761 attcctaatt cctgattatt ttatgagaag cattttcttt acatccgtaa attcactcgt
     5821 cacaatttta taataataaa ttccgcttgc aaatcctgca ccgtcaaagt ccactttata
     5881 agtacccgga ctcagattct cattcagaag tacattcact tctttaccta atgcattata
     5941 tactattact ttcacaccgc cgctttttat aatatcaaat tttatattgg tcgttgggtt
     6001 aaaaggattt ggataattct ggtacagact gaatttatca gggacaagtg aactaagctg
     6061 atttattcca actgtagtag tccatcttgc agtctcaacg attggtactg ttatattatt
     6121 aatggtgatc acgctgtcca gtccggtact gtcaggactt gtatatgagc ctgctccaat
     6181 ccctgtccat cctctgaaat aataattcgt acctccgctg ttgatcactc ttgacaatac
     6241 accgatctga ggacttgatg cagagtcata atatactcca ccgccaaatg tatttcctac
     6301 agatgaagta attaaaagtc tgtactgagt tttgtaaaat gctgtcagat tagtattgct
     6361 gttgatcaca attgtctgac ttgtatcacc tgaatttgac cagtttgtaa atacatactg
     6421 tgtagctccg actactcttg gactgatcgc tgttacgttt acgcttgctc cgtttgtgaa
     6481 tacaagctgc tgtgtctgat tataaacaat tccgtttacg ctgaattctg cagtaccagg
     6541 tctgttcgtt ccaacagtta aaaatgaaga gttaactaaa acacttacat acctgctgtg
     6601 gatcggagtt ccgtttggac ctttgccgat cactttgata ctgtatcttc cgggtgttac
     6661 acttccgact gcagcaacat taatgctgac agaatcagga aatgaattta ctgaatcttt
     6721 tccgacaaaa gaaatattta gtgaacctgc agcgggaagt gtatccatag ttgctgtgaa
     6781 ttttactact ctgccgtaat cttttacagc agggactatt gctctgaagg ttacattctg
     6841 tccgctgttc actgatattg actgtgaact tgtctttaaa gcaaagtcag gataatatcc
     6901 tacataactt ccgaaattat tatttctgaa atctgtccag gtaagcattg cagtcactga
     6961 atttgaagtg atcgaactgt aatctccaag ataggcaggt gtaccgccgc cgccgcaagt
     7021 agtacagttg attctgaatt tcttatttga gatcatttta ttctgaacaa aagttgcacc
     7081 gccgtcagtt gaataagaag cataaacaag cgcactgtca cttgtcggtg tatctctggt
     7141 gtcattccac tgaacataca gtctgccggt ttctttatcg caccaagttg aaggatgcca
     7201 ttggttatgt aatactgtat ttggatcatc attaattctg acttctgatg accatgtagt
     7261 tccatgatta gtagaatatc tgcagaaaat atccggttta tttccgtcac ctgcaggagt
     7321 attggatgaa tataccagat aaagtcttcc tctgttagga ccgaaactgt tatctgcagt
     7381 aataaaagga taaggtcttg ttctgaaatt ttgaacagaa tttcttccgc ttactgctgt
     7441 gccgacatag tttgcaaaat tctgcgatga cataaaatca aaagactgcc ctccgtttgt
     7501 tgatctgaaa aatgaatatg tagcagcaaa tgaattaccg gtatttgtaa ctacataaac
     7561 gcatccgccg gaaacatttc ctccgactgt gtttgcgccg acagctacca tcatacccgg
     7621 aagattgtta cttgctgaga aaacaagatt ataacttgca cctccgtctg tacttctcac
     7681 tacatttccc ggggtcatca caccataaat ataattggaa taaggtccgt tagtctgatc
     7741 tgcagcgatc cagtttttgt catttccgat atttgcagag acaggtgaaa gccagctaac
     7801 tcctccgttt gttgacttta tattccatgt tcctgtgata ggacttttca tattgtcata
     7861 atacagatta ccaagactgt cataagcagt cacaggatct cctgcctggt ttgggaagaa
     7921 tggattgttc ctctgccagt caagtccgtt aagtgtataa taagtagcat ttgtattata
     7981 agctacaaaa taccagtttg gatttttcgg actggaagaa atatgacttt ccgaaaaatc
     8041 aactccgaga tcaaaattat caaaacctga attatcggtt atgacagtta cttcacctgg
     8101 cattgatccg gatacatgat cccagtactg cttaggcggc tgatccagat ttggatcgtc
     8161 gttattgccg agtatattaa atccactgag ataaatactt attacaaaca gcggaataat
     8221 ataaaaaaga gttatctttt tcattagttg agtccctttc gtttatattt tttagatttg
     8281 ttttttactg atgaaatttc aataagttat gcatttatat ttaatctaac aattatatta
     8341 ttttttttaa tgaaagtgtt cagtggggaa ttaaatttta cctcgaagtc tcagagttta
     8401 atattcatta ttcaaagaaa taaatgtaat tgattttgcg ggaagtggag cgacgaatca
     8461 aaagtaaaaa tccgtcatgc cctagtgaat ctttcggcat ctgataattt tatatttgaa
     8521 tcagtgttct tataaattag cttcacccga atgaatccgg attcttaaag acataaaaaa
     8581 aatcccgcaa agaaattttc atctctgcgg gaaaatgtaa atcttaaaaa ttcttaattc
     8641 ctgattttta atacttaatt attttatcaa cgccattcgc tttgtctcag aaaatcctgc
     8701 cgtttcaagt ctgtaaaaat acattccgct cgaaagcgaa gctgcattga aatcaacgtt
     8761 atagcttccg ggtgaaagat tctgatcaat taaagtatta attttttcac caagcatatt
     8821 ataaatgctg agcacaaccc gctcattttt tacaatatca aattttatat tagtacttgg
     8881 attgaacgga ttaggataat tattatataa attaaatttc tcaggtaata cactcgagat
     8941 ctgatttata ccgacaggca ttctccttac tgtaattata aaaggattgg atgttaccga
     9001 atcagctccg ttgaatgcag ttgctctcca tgtcatgatc actgaatctc ctgttgtacc
     9061 ataagatgct gccagcgaat caaggaaact gtttctcaaa ctcatcacag tatccagtcc
     9121 gttattatca cttgcgaaaa tatcttcagt aggtgtagcg acctttttga gcttgaatct
     9181 gtaaaccaca gtatttgaag gacttgaaga tgtccattta aattgtgtga tattaggatc
     9241 attcggactc actactattc ttgacaaatt aaccggtgac tgtaaagtaa tcggacttat
     9301 aggtactccc tgtactttaa tataagctac gatagttttc atgttagctc cgaaagcatt
     9361 tgatgtcgtc agagatactg tataatttcc tgtagttggg aaatttatat tacccggatt
     9421 ttgagcagtg gatgtagatg gtgtagcacc ggggaaactc cagtcccatg tcgtcggtcc
     9481 gttagttgac agatcaaaga aatttactgt gccgcctgtt attggcagag tctgtatatt
     9541 agaagtaaaa tttgaaacag gagctactcc agtaacagca tcaattccgt caattgtcat
     9601 cacaccagaa ttgtttggat caagccactc tttaagctgt gtggcaggtg taccgccatg
     9661 tgcccatgat tctgaaagct ttccaaaata tttttgtaca ctttgaggat ttgcacaatt
     9721 tgcaggagta ccgccaagat tttgaccgat cactctttta ttctgatcat aaatcgggca
     9781 tccagaagat ccgccttctg tcattccttc gtcccaaact acaagccaga aaccgtttgt
     9841 aagtctgccg ccaaaaccgg ttacagtatt tgcaggctga ttatcaacag agatcttctt
     9901 tactgctccg tcaggatgat gaattgcagt ctcattcagc ggctgtgtat ttgatctgtc
     9961 ccatccattg taatatgcat taaatgaagc aggtacagat tgatttaata atactaatct
    10021 gaaatcagta tcaaaattcg ctgcctttaa tgtcgcaccg gtcattgtat ttgcaagact
    10081 tccaagtgta cctatacatg taggtgtttc ataattataa taaaaaacta tagtagaaaa
    10141 attatccgtt gcacaatgtt cagcatataa ataatatggc tgtctgtcct gtaatgtatt
    10201 attcataagt gcacctgaac ataagaatga accgccgcct tgtgagaatg tgatcctcgt
    10261 tactgatcgt ttttgctcta cccagggaga tcctatcggg caatttatat taatgttgca
    10321 tggctcttca agaacactgt tatatccgaa gatatcttta tatgcatgtg tgactttatg
    10381 caaagtgatc ctgcctttgt tttttgaata tacaggttca taatattcaa ggattgttgt
    10441 gcttccgtaa gtaggagttg tcgcgaagta tccgtcttca gaattattct gttcagtgaa
    10501 tgctccgtag attgtgtttt ttccaacgtt gtaaacaaaa aaagtagcac cttttggcat
    10561 gtgaaattct ttgaatgcaa ggttaattga tactgcatct tctgacctga ctgcaagtct
    10621 ccatactctg cttccgtcag aaagtgtttc ccatgttcct gagttattca gattataatt
    10681 tacatcgaag ttatatccat atctgaaagg catgcccggt tctttttctg tgatctgatc
    10741 ttcttcgatc agtctctgaa cgtcaacatc cggcattgtt accgaactga atacgtcgct
    10801 aagctcagtc attgagctca tcggctgacc accgacgttt atctgcgcat gtaaattatt
    10861 tgaaaacatt gcgcatacta taagaaggca agttacaaat aacacttttg caaatctgaa
    10921 ataatttttc atgattttta atttattttg aaatttattg ctaaattttg ttcaggttaa
    10981 ttgtaagaat ttttaaatta taaattattt acttaacatt caaataaaat ttctcacaat
    11041 agtcttttac catcctgttg gtattgaaag tacctgccag ctctctgatc gaattcttta
    11101 tcatctttac ccactctgcg ggtattcctt tgctgtttct tttgtagaat gtcgggatga
    11161 tctgcttttc aagtgtatca taaagactca tggattcaaa gagatccctt tgctcaagcg
    11221 gaatattctc atcagcaaga ctttctattt tccaaccgtt gccctgattg tatgcttcat
    11281 cccaccagcc gtctaatata ctgaaattca atccgccgtt tgcaatcacc ttcattccgc
    11341 ttgtacccga agcttccttt ggtctcctag gattattcag ccaaagatcg catccctcta
    11401 caagatgtct cgcaatattt atatcatagt tatcaaggaa taagacctta ttctttagat
    11461 cttcatcttt tgtaaatgcg actatctgcg aaattagatt tttacctcct tcatcttttg
    11521 gatgagcttt tccggagaat acaaactgta caggtttttt actattgtta acaatttttt
    11581 ccaatctttc gagatcgctg aatattaaat tgcctctctt ataagtagca aaccttcttg
    11641 caaatccaat tgtaagagca tctctgttta atatgagttc ggcgtctttg agcttttcct
    11701 tatctgctcc catacttatc cacttctctg ttattctctc tctgcagaaa tttattaact
    11761 gatatttatt caatagtctc aaagcccaga tctcctcgtc aggaatttca tctatcctgc
    11821 tccagatatt ttcatcatac ttccagtctt tagtgaaata tccggtaaag agtttatttg
    11881 tatgaatgga aacataactg gctatatgaa ctccgtttgt aatatgatcg atctgtgatc
    11941 tcgtctttgg cagcttccac atatctcttg acacttcacc gtgaagtttg ctcacgccgt
    12001 ttaataagtt agaattattt attgctagaa atgccatatt gaatttgtca ttatcagcct
    12061 gacctttatt aagatcacct tcgctgaaaa gtttttccgg actgactcca acttcctttt
    12121 cagcatattc cgtaaagtat ttcataaaaa gatcctttgt gaaaatatct attcccgcgg
    12181 ggacaggtgt atgtgttgtg aaaatattcg agccgtaaca tttggctttt gcttcatcat
    12241 atgaaatatt ttccgatttc attttatctc ttattctttc aaaacagaga aaagccgaat
    12301 gcccttcatt caaatgatat gcctttatgt caaagccgag tttttttaat aatctgaaac
    12361 ccccgattcc aagaagaatc tcctgttgga tcctgctttc attgtctccg ccataaagta
    12421 tgtctgtgac ccttctgtcc tttacagaat tttccggaat aaatgtgtca agcagataaa
    12481 gttttacccg cccgatcttt gcaacccaca cctgaaaata gatcgccctt ccggggaaat
    12541 taactgcaac tttcagggga atgtaatttt catctctgag aagatatatt gggagatttt
    12601 taaagtcatt cagttcataa ttttccgtct gccatccgtt ctgatttata agctgtgtaa
    12661 aatatccgta attataagcc agtccgattc ctgcaagcgg aattccgaga tcagaagatg
    12721 atttcatatg atcacccgaa agtacaccga gtccgcccga ataagtcttc agacattccg
    12781 taattccaaa ctctgctgaa aaatatgcaa tgatcggatc ttcagtttta aaatactttt
    12841 catcgaaata agtgctcttc gacatatagt tcttgtaatc agcataaata tcatttatcc
    12901 tgctgccgag atttttcttc tctaagataa attcaagata acttggattg atctcattca
    12961 gcaccatgac gggattatgc tgtgaccagt tccagaaatc cggattaatg tcgttaaata
    13021 attctactgt gtccttgctc catgaccacg agagattgta agctatttcc tttaacttta
    13081 gtataaggtt ttccatcaaa agattttgtt aataatcttt caatataaaa tttgtaatac
    13141 tttattacta taaatttttt tttgcaaaaa tcagaaaata ttgggtggta aatgtgataa
    13201 tgctctgaga ttcgaaggct tgaatgtatt aaatagggat atttttattt aattaacagc
    13261 atttgttttt gatcaatata acttcctgct aataatttat aaaaatggtc aattgcaata
    13321 acttcatctc tgttattgcc tgttccgtta tatctgatta cccggttttc ataaatctga
    13381 gcgaaggaaa gatttgtaat gaatagtaag aaaaataaga tttgacttcc catgataaat
    13441 aaccttaatt tttttggaaa atagaaacac tttttttatt gatgaaatct aaaactgata
    13501 atttctataa tcaatttacc ttttaatctt ataggcaaag actattctgc taagatttct
    13561 aaattgagaa agtttaaaaa aaatggatgc ctcaatagag cgtgaaagat ctttgagcat
    13621 tcaggatttc ggggcattca ctattctact tcatttctaa attgtaatta ttcacaatat
    13681 aaaataagtt tatgcacatt atttaaaatt ttaaattctg aaaaaaagag aatcttctaa
    13741 aaagtccgtc agtcctccaa aaaaaggtac tgtaattcat attcctgctg aaatttcatt
    13801 aaaagaaatc gtcagatcag tatggctgcc gtcacttata tcagttttcc ttctgataat
    13861 tttattttct ttgagaaaga tcgcaaacta tgatcttgga tttcacctcg caggcggtag
    13921 gtggattatc gagaatatgg cttttcctgt gaaggacatg ttcacatata ctgtatcagc
    13981 aaatgattat ctcgacatcc aatggctgta tcaggtagtt atgttttctg tacagagctt
    14041 tacaggagat cacgggctta caatttttaa tgctgctgcc atcacatttg tattctttct
    14101 gatgcttaaa agaaataatg tcaacgatgt ttacattccc gtttcgctga taatgatcct
    14161 tgtcactctg atctgcattc aggttagatt cagctaccgc ccggaaattt tcacatggat
    14221 atttatgctg ctgatgctga tagtgcttga cagaaatttc ctttataaag ataaaattaa
    14281 aaaaaccggg ctttattttc ttcccctgat aatgctcttc tgggtaaata tgcacggact
    14341 ttttataatc ggatttgtga taatgatctc ctacattatt tcaggttttg tgcgtgataa
    14401 aaagtttgac aaagatcttt taatatggac tggaatttct ttcgctgcag tttttataaa
    14461 tccatatttt tctgaaggag ttttatttcc tttctatctc tttacccgcc ttcaaaaggg
    14521 aaatattttt cagataaata ttctcgagct tcagtctccc tggggcatga cagatattct
    14581 gccgactgag ctttatattt attattcagt ttctgtgtta gcatttatac tgcttttcat
    14641 aactctcaat cacaggaagt tccatgaatt tgcactgatg tcagcattct tttatctatc
    14701 attctcagca ttcagaaaca tacctctttt tatgatctat gcatttttta taatttcgat
    14761 ttcagtatct gatcttatcc gtgaaaaaaa tattttcagc aaaggaaact ccgtgacaaa
    14821 attttcaaaa gtcttatcta taataattat aactgtaagt attctcaccg gcttaagagt
    14881 aattacggac gcatattata ttccatacgg ctctgagata aaattcggaa ccggtgttga
    14941 taagcttgca ctgcccgata aagcttcaga ttttataaat gaaaatacac ctgacggaaa
    15001 gatcttcaac aacctggaat caggcgggtg gcttgaatgg aaaacaggac ggaaagtata
    15061 tattgacgga agacttgaag tgattcgtga agatttttat aaagaatatt tatcgacaat
    15121 aaataatgca gccttaaatt ctgaattaaa aaaatataca ccggagctga ttgtaaacag
    15181 tattttaaat ttcaactgga caaatcagct gagtcagatc agggaatatt cactttcgta
    15241 ctgggatggt atttccgctg tatataaatt acaagaacct tcggatacgc tctctgcatt
    15301 tagttttaaa tcaggtctga taaatgcagg aattgataca ttgggttttt ccgaaaccga
    15361 aaaggacaga ctgctgagga cagtcagaat taaaaatctg gaaaattgga tcagcggttt
    15421 ttacagcttt agaaaaaatc cttactattt aattccaatg ggaaattttg catttgaaag
    15481 cggggagttt cagacggcag aacttcttta tctgaattat ataaaagaag gtgaagggtt
    15541 cataaatgaa aatttattta aagatatttt tctgaatctt ggttcatatt attacaactc
    15601 aaatgatatg gcgagagctc agtactgtct cgaaagatac cttgaagaaa atccgggaga
    15661 aagagagatc ataaaaaagc tgaatgaaat taaaaagagt aggaaaaatt aatttgaagc
    15721 tgtctgtaat tattccgtat tttaatgaag aaaaaacaat tgagatttta ataagaaaag
    15781 ttatcgctgg agatcttgct catgagatca tctgctctga tgacggatcc actgattctt
    15841 caaacatgtt ggtctttaat ttgaaaaatg aatttcctga tctgataaaa atttcaggca
    15901 gcagtattaa caaaggcaaa ggttcagcag tgagaaaagg tctcgctatt tctacaggag
    15961 acataatcct gattcaggac gctgatctcg agtatgatcc gtcacagtat tctgaattgc
    16021 tcagaccatt tgaagatgat aatgtaaaga tagtttacgg ctcgagaaat cttaacggaa
    16081 atcctcattc ttctttttca ttctacttgg gaggagtaat tttgagtaag attgtgaata
    16141 ttctttatga ttcaaaaata actgacgagt ccacaagttt tgatctggaa tgtgacggat
    16201 ttgaattttg tcctgaagtc acttctaaag ctttgaaaag aagttttaag atcactgaag
    16261 ttccgatagt ttacacgccc cgctcaaaaa gtgaaggtaa aaaaataaaa tggattgacg
    16321 gtgtaattgc agtatatacc ttactaaaat tcagattcag aaaataattt catcagtcga
    16381 tcttttgcca tacagctaaa actttatctc cacttttaag atataatttc atattctcaa
    16441 tttcaaaatt atcactttcc tttaatgcgc tgaatattct gctgtcataa ccgcccggac
    16501 acatcattct tgtagatgcg attttactga aaaaaataag agaaccaata ttcttcatac
    16561 tcccgttcat tttattacat ccggtattcc ccataaaact attctcctta atatttattt
    16621 cgatcatcgg catacctgtt ttaaaatcgg atttattaag ttcttcgtca ccaagttttt
    16681 ccaacaccca gatatcattt atcctgtatg ttcctgaaga atccctttct gttgtgaaat
    16741 ctccgcaccc tacatataac atatcatcaa aatttaattt tatttcatac ggaaatttct
    16801 ttccgctcat tagatctgta cattcccttt ttaaaattct gactataata tcatcaccgt
    16861 caccttcagc aagtatagta atgctgtcgt ctctaaggtc attgattgtt tctatgactt
    16921 taaaatttat gacttcaatt ccggtattga aattaaacag caatgattcg gcacctgtaa
    16981 actccactgt ccaaggaggc tcatgaccgg atgcataaaa tccaattcct ttatcaaaca
    17041 tgatctgttt cacactgtct ctgattatac tgccggctcc ctcaattttt tgaaagacag
    17101 ctctcacagc ttcatcagat ttcaaagaca aaattccatt atctataaaa taattattgc
    17161 aattctgaag tgcatcaaaa aattctccat ccttctgata ctgacatgcc atttctgtcg
    17221 ctagtatatt ttcgaatttg atcttctctc cttctgatgt gactttccct gaaaaagtat
    17281 tgcagcctgt atttccgttc accatcctct ccttaatatt tatctcaatg aatggtaaac
    17341 cataggtaaa ggaactagca gtgaccgctt cattatctat catagtcaat tcccatttgc
    17401 cattgagaaa agtttccgtg ccttcagtcg gctgggaagt ttccatttcc tgtgagaatg
    17461 agacccctga ttttaaaaat aaaataaata aataaataaa tataaaagtt gtatgtgctg
    17521 aaaatttcat gtgtgaattg attttaaaat tcaaaagaaa ttactgaaat tatacctttt
    17581 aaaaatgtcc atatattgaa attcaaaatt actttttcta atttgtataa attcagatct
    17641 tataaaaagt tttattcaaa ttccctaaaa taatctatta agtaaatgaa aaacctgatt
    17701 ttaattttaa caattatatt aatttccatg aatgcatttt cacaaaatat ttattccgac
    17761 aaacttgctc atacttattc catagtcgcg cgtgattctg taacagggga gatgggagtc
    17821 gctgttcagt cacactggtt ttcggtcgga tccattgtta gctggggtga agcaggcgtg
    17881 ggagtggtcg cgactcagtc ttttgttaat ccttcgttcg ggataaaagg tctcgaactt
    17941 ttaaaatccg gaaagtcacc gcaggaagct gtcgatgaac tgatcgcagc agacgatgga
    18001 agagatgtca gacagcttgc aattcttgat gtaaaaggaa gatcagcttc atatacagga
    18061 aagaactgta tccccgaagc aggcagcatc gcaaaagata attattccgt gcaggcaaat
    18121 atgatgctga ccgataaagt ctggggagca atgtccgaag cttttgagaa ttcagccggt
    18181 ccgcttgcag aaagaatgat agccgcactt gaagctgcac aaaatgccgg tggagatatt
    18241 cgtggacaac agtctgcatg tttacttatc gtaaaaggta aagctaccgg aaatccgtgg
    18301 gaagacaaat ttatagatct ccgggtagat gataacacgg aggcagtgaa ggagataaaa
    18361 cgtctgctga aagtttacag ggcttatgaa catatgaata aaggcgatct tgcaatcgaa
    18421 aaaaatgata tggatcttgc gatgaacgaa tattcgacag cagaagaaat gtttcccgaa
    18481 aatcttgaaa tgaaattctg gcatgctgtc actcttgcta atacaggtaa agtcaatgaa
    18541 tccctgccga tgtttaccga agtttttttg aaagataata actggagaat tttgtttggc
    18601 agattaaaac cttcaggact tttgaaaata agtgacagtg atgtgcagac tattttggat
    18661 ctgaaataaa ttcaattgtt tccgcagagt cacgtgctct taggactctg cggaaactga
    18721 gatactgagg ttactaaatt aaataaatta catttttact tttatgagtg aattacttat
    18781 tcttacagga gcatgcggag ttggaaagtc aacgattgca aaagaatggg ctcgtcgcaa
    18841 aaatggtgca ataatcgaat gtgattattt tacggaatgg atctatgata aaaattttat
    18901 caggttttca tatgaagagg aacttctgac tgcgcggctg tcaataaata ctgcaaagga
    18961 atatcttaaa ctctcaatgc ctgtagccat agaaaatgtc tggactccgg ttggattaga
    19021 atttctgaaa gaagaattat ccgatttaga atatataacc aagcagaaat ttatctggct
    19081 gaaatgcgaa agtacagaaa accaccgtcg ggatgaactt cgtgtacccg gggatgtaat
    19141 gggtgagaga gtagacattg ttaatgacca gcttaacggt tatgtctggc ctgcttattt
    19201 gcatattatt gatactactt ttctgacagt aaaagaaact cttgaacaaa ttgcaaaatc
    19261 ataataattt aatgttaact tatttcgtaa ctaaacttca ttttaaaagt atattttttt
    19321 tgttcgtata aaattttaat ttactaaacc tttttattaa ccaaaccaaa ccaaaattaa
    19381 aatgaaattg atagatgcat tacttgctga atttcagtat gaatctcagc ttacaagaaa
    19441 catgctcgac cgcctgcctg acgggaaatt tgattacaaa cctcatgaga aatccatgac
    19501 actcggtcag cttgccaatc acattggaga tattccaaac tatgtgggtg gtactttaga
    19561 tgtcgatgaa ctggatttta gtaattcaga ttataagatt ccgaccaatg ctactaaaga
    19621 cgaactggta aaagcttttg atgaaagtgt tgcaaaagca atcgaaagtt ttaagagaca
    19681 tgatgatgat gaattaatga aagagtggac tatgaaagac ggagataaag tatatatgaa
    19741 aatgccgaaa gctcaggtga tccgtgggtt tgtattaaat cataatgtac atcacagagg
    19801 acagcttcag gtatatctga gaatgaatga tattccactt ccatctgtgt atggtcctac
    19861 tgcagatgag caggcaatgt aattataata tcagatttat tacagcggtg aaaaataatt
    19921 taccgctgtt attttattac aagttttgct gtaaatgatg agagtgcgat tcccattccg
    19981 ttacatgaaa caatacattt caccctgtcg gaaatttctt caatgaccgg tagtttattt
    20041 tcagtaaatc ccattatccc gctccatgaa taatcaactt caaaatcctg ttcaaataaa
    20101 attgtattct taagcttctt tttcagatct gaaattattt tttcactgac atcaaactcc
    20161 gtagtttctt cattttcaaa atcaagattt ctcccacctc cgaatataac tctgtcacca
    20221 aaatttctga aataataaaa tccttcatcg aaatggaata caccattaaa tttaagatcc
    20281 tttacaggct tggttacaac aacctgtcct ctgccgggtc tgatcttcag tgagggtaaa
    20341 aatttttgtg tgaatgcatt tgcgcataca gttaaggatt tgcaattgaa aactatatta
    20401 tctttcacgt caccgctgtt tacaaaaagt tttacataat tttctttctc gctgtaagag
    20461 tctgcattgc atccgttaat aatttctacg ccaagcgaaa cagaatatct tatcagggac
    20521 ttcatcattt ttcccgtatc tagctgtgat tcaaaaggac tgtaaataag tgaagatact
    20581 ttagaaggat taaatccgaa ttcttttatc cgttcatttt ttataaaata aacatcgtca
    20641 ttaaaaatgt ctttaagctt tacatttaca aagggaattt tatttaattt ctcagaatgc
    20701 ttttcatcta taatttcata cccgccataa ttcagaaaac caatattttc atcaccaagt
    20761 ctctttctta aaataccgat tccttcccat ctgttggaaa taagttccac agttttatca
    20821 acacctgtat attcaaaatc agacagtatt tccgtaaagc taccaaagca tgcaaaccca
    20881 gcattcttag tacttgcgcc ctcaggaaaa attcctttct ctataatcag cactgacctt
    20941 tcaggaaaat tctccttgat ctcacaggca acagacaagc ccagtattcc actgccaatt
    21001 attatatgat cgtaatttgt aaatgaattt ttttcccaga aagaaagcaa nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atttttatac acacattctt tgtctcagta
    21181 aaaaatctca acgcttctaa tccgccttct cttcctactc ctgaattttt cacaccaccg
    21241 aatggtgttc tcaaatccct taccatccag cagtttatcc agacaatacc tgattgtatc
    21301 tttgcggaaa ttctgtgtgc ccttgaaaga tcacttgtcc atactacagt tgcaagaccg
    21361 tactcagtcg aatttgccat attcaaaact tcatcctcac tttcaaaagg cattatagta
    21421 acaaccggac cgaatatttc ttcctgatta ggactgcatt tataatcaag accttctatc
    21481 acagtcggag ctatgaacca gccattatta catctgcctt tcaattctac cgactctccg
    21541 ccgcacagta tatttccgcc ttcttttttt gctaggtcaa tgtaataaag aattttattg
    21601 taatgctgtt cggatactat tgcacctaca tttgtatctt ctaaaagcgg atcacccaca
    21661 ttcagttcac gaactttttt tacaaagtca gttttaaact tttcataaat ttccttttca
    21721 ataaatatcc tcgacccgca gagacagatc tcgccctgat ttgtaaaaga agatctcact
    21781 gtagtttcaa gcatctcatc atagctgcag tctttaaaaa tcagcacagg atttttaccg
    21841 ccgagctcga gagataattt tttcagcatc ggcgcagcta ttcttgatat ttccataccg
    21901 gtctttgttc cgccggtgaa tgtaatggct ttgatccctg gatttgaaac aattgctgaa
    21961 ccggctttat gtccgtatcc gtgtatgata ttcaaaaccc cctgaggaag tccggcttca
    22021 atgcaaatct cggaaagcat gaatgcagtc agaggagtta cttcagatgg tttggcaatt
    22081 acacaatttc ccgctgctag agcaggagct attttccagg taaataaata cagaggaaga
    22141 ttccacggcg agattgctcc ggcaattccg aatggctgtc ttaaagtata attcaaaatt
    22201 tcagaatttg tatgatgtga ctctgatgaa aattgtgtaa tagccgatgc aaaaaattca
    22261 aaattcttaa ttgctctcgg aatgtccacg ctcaaagcga gctgcagcgg ctttccatta
    22321 tctttgcttt ctgcatatgc aaatttttca agatcccttt ctataagagc agctatttta
    22381 taaagatgat cagctctcct gtctgcagat aatgcagacc attttggaaa agctttttcg
    22441 gcagactgaa ctgcaagttc cacatcttca gcatccgaat cgggaagcat tgtatagact
    22501 tcaccggtag acggatcata attatttata taattattgg aataaggagt agtgagaatt
    22561 ccgtcgatat aattttttag tttatccata tacttttact tttattctac ttgttccagt
    22621 atcgtgcgga atgttgtgaa tttatctttg tatttatttt gaaaatcagc ctgcagcgca
    22681 ggtgcaaatt tatcacgata cttttcataa tcttccatcg atctcagaaa atattgaatc
    22741 gaataagttt tttcttcagg ggaatccgtt aatattctgt aaattttata ttcagtaaaa
    22801 agacctgtct ccatcacatc aggaatatgc ttatccatca tccatgacag ccattcatca
    22861 tgaacagaat cttccgtaca aactgtaaca ttatataata tcataataat atttatttac
    22921 attcaaaaaa tgaaaaaatc attaaataaa ctcttaactc tgatcaatct ctaattcata
    22981 cactggtatt gccagaacca aaaagaatta ttaaacagtt attgctcctc ccttttgtcc
    23041 aaaggggagg ctggtagggg tttgtagaat tgagtttgat ttgactccac tttttacagt
    23101 gctgagtttt tatatttggt ttttgctttt ttttcgccaa caaccccctt cgctccccct
    23161 tttttttgtg ggaaatcact aattaattat tctgaattgt tctattaaaa atttatataa
    23221 acttatctaa ataattcata gtgaactttc ttgcaatttt cctgtaaatt aaagtaatta
    23281 atttaactat taactattaa ctattaacta ttaactattt gaaattctta attcctaaat
    23341 tttaattcgt aatggttcta taaacatccc gttcttccgc catcaaccgg taaattaatt
    23401 ccattaatac ttcctgcgag tgatgaagct agaaacgtaa ctgcataagc caattcctca
    23461 ggttcagcaa attttccaac aggaatttct ttcagcattt cttttgtgac ttcttcaatc
    23521 gtcttacctg atatcttaga tttattttca ataatagatt ttaatcgtac cgtttttgta
    23581 gcaccgggta atacgttatt tactgtaatt ccaaatggac cgagctcgcc tgcaagtgtc
    23641 tttgcccagc ttgcaactgc accgcgtatg gtatttgaaa ctcccagatt tttcaaaggc
    23701 tgctttacag atgtagaaat aatatttact attcttccat atccttcctt cttcattccc
    23761 tcaactactg cctgcacaag aatctgatta cagataagat gatttgaaaa tgcctgtaaa
    23821 tattctgaag tgtcagcagc aattgcctcg cctcctttag gaccgcctgt attatttact
    23881 aaaatgtgta caggtgaaga ttctgaaata aagttatgca ttttttcttt caatgaatcg
    23941 ggatcagaaa aatctgctac tataaaatta tgattctgat cttttgctgc aggtagttct
    24001 cgtaatactt ctctaagaga atcttcattc ctcgaaatta aaatcacact tgctcctgat
    24061 gaagccagtt ctatcgcaat tgctttgcca attccctgag tacttccgca gacaattgct
    24121 tttttatttt taagatctat gttcaatttt tttaaatttt tatttattta aagtagctgt
    24181 ataataaaag ccaagttttg ttttcagatc ttccggtaat tcaggtagat ctgatcttgg
    24241 tatcatatat gtaggcagat caaaaaggtc tttgaatacc ttatgttttt ctgcagtctg
    24301 cagaagatac tgatgtcctg aagatccacc tgtgcctacc tttgcgccga ttattctgtg
    24361 aaccataagg gcatgtttgt atctccaggt tgtaaaatgt tcgtcaatgt ccacaagata
    24421 tgtcagcaat ctgtatggca gatgaagtat gggttcgtct ctgtaaagat ttatgaaaag
    24481 tgctgcatgc attgatctgt atgaaagtct tctatgcccc tgtgatctta actcttcata
    24541 ttttttttca tcaagaagcg cttcaaaatt ttcttttgta gattcaaaca tttcaagctg
    24601 ttttactttt ctctcttcag taagactcgg gttatttaaa attatatttt tttcattctc
    24661 aagagtcaca ccaactgcat ccttataata tttccaaaaa tcaaaatcat caatattaag
    24721 aaatggaatt cttgaaagcc atttgtcaac ggattcatat atcgaatctt tactttgaga
    24781 atctatcaac attttctgat gctcgtcaga caccatagta taataattct tgtcattgaa
    24841 ttttactctg ttgtcaatat tcaatccaag tgtgttctca attaacctaa actgaaaact
    24901 ctgaaatccc gaagccggta tcagataatc tctgaactct aaaaaatcta atggtgtaat
    24961 tgtctcgacc actccaagct gatcgatcag aattttctga atgacagtaa ccctgcgtaa
    25021 tcttgatact gcaactccga tatttctttc atcaatatca tccttgcgaa acatttcgga
    25081 aattgaattc agctcatgca ggatctgctt gaaccaaagc tcatatgcct gatgaacaat
    25141 tataaacaac atttcttcat gtgcaggatt tccgtattca tcgctctttg gtttttgact
    25201 ggtaagaagt gaatcaagtt ttaaatagtc tgcataataa acaggtggaa atggtttttt
    25261 cattgtgaga atttttttgt ttaatcaaaa tagaattatt gaatgattat ataaagtaag
    25321 ttttatatat ttaaacttct taaaacagac attcaaaact ttataatata atgagaactt
    25381 ttatctcact aaatatagac attagtacaa aaaataaaat taaagaattt aaggaacaga
    25441 tcattgaaaa tatcatgtct tcagatcctg aaattttaaa ttatttaaaa tgtgaagacg
    25501 aaaaaaatta tcatattaca atttttttta taggggatac taataatgta atgttgaatg
    25561 aaattgaatc cgcattaaca gaaataatat cagacagtat tcctttgcag ataaaaatga
    25621 agacaggtaa gttgaatgcc tttccaaatt ttaggtatcc gagagttata atacttgatg
    25681 tgatagatcc gagcaacggt ctgaaaaatt tgtcaaaaga aataaataaa tgtatgttga
    25741 aatttggatt caaatctgat aagccatttc atccacacat tactcttgcc cgtgtaagac
    25801 gtgatagaaa aattaatctt acttctttag gtaacatact gttaacgcag atagaattta
    25861 caacagatca ttttagtctg atggaaagcc ggctcaattc gtcaggagct gagcattttt
    25921 cttttacgcg atttcaatat tcagaatcct taaatctgta ttaatatatt taccatatga
    25981 aaaaattatt tgcaatttca ggatgataaa atatattaaa aatgcagact tcattttata
    26041 atttttccgc attatgattt tgaaaattaa ttttaaaaaa aagtaaataa ttatttaagt
    26101 tgtgtatcaa gtttactctt atttatcgtt tactgaaaca attcatttgc acagcaagat
    26161 aatgaaacat cttgtgtaaa aacggaagaa gccgccggca gtagtatttg cctgcctgag
    26221 atctcaggta tgaatgaatg ttacgaactt ccgaatgtca gagagagaat ggataaatat
    26281 gaagttgatg caaattcaat atcaggtgta tctgactgat gatatttatt ccaatacaga
    26341 aaatttagaa agcctttcta tggaagaata ttttaagatc tatgtggtga ataatatgaa
    26401 ggataaaact tatggaactt ctgagttaaa gcagaccgga gaaataatgg gaaaaaattt
    26461 tattgctgac aattggaaaa cctaaagtat cagattgaac aaaaaagtga taatctgacg
    26521 atcggcgttc cggtaatttt cgaaagccat atacatgccg ataatgcccg atctcatctt
    26581 ttgattacta aagtaagcaa tgacgttact taacaggtga tgctgatata tatgaatatc
    26641 attctcataa aaaaaaaata tggcctggat attataagtt ttatgaaaat gaagatccat
    26701 tcaaaagatc agatcggcaa gcgacagtgt ggtgatgaag cttattgaag taaatagata
    26761 gtttttaaag catacaataa tacaataaat ttaattttta ttctttaact atttggctgg
    26821 agacagacaa atttctcaaa ggaacattat tatagattga tactcaaagt aaaaaagtgt
    26881 aatttgtgta ttaaattttt ggaggacagg tagctcagtt ggtagagcaa cggcctgaaa
    26941 agccgtgtgt cgccggttca agcccggctc tgtccacgcg cttcaattaa gaattaaaaa
    27001 ttaggaatta tgaatttttg taattattaa ttaaatttct aataatatat taattgataa
    27061 tatttattaa agcattgtga tttatttctc agtgcttttt tattcagtat tacattattt
    27121 gaaaaactga tttcaggcag gattctcacc ggtaaatttt catttcattc attttattac
    27181 taatacagac attcaaccaa ttgaattgtt tccccaaaat ccatgtccat atattcccca
    27241 aatattacaa attactggtg attagttagt tttattatat aattacaact tttttcatgg
    27301 tatgatattt gcttttaata tagtcaaatt gatgaactat aaattcaaat taaagaatca
    27361 tgaaaacatt aatactaaat attgcattaa tacttgctct gacattcagc caggcatatt
    27421 cacagcccag agcaaaaata tttaacaaag caacaaatcc agagatgctg ttatcaatac
    27481 cggcgctagt tccggattca tgcgttgcat cgggtttagt tgcagtaggt aacggacaat
    27541 acgtttacct ttcagtattt aatttcggtg ataacacagc catcacttca gaaagctgga
    27601 cattcatttc aaaacctgca gggtcaaatg cgcagctaat ttcaataccc agtctgcaat
    27661 ggtataaatt ccgcacagac gaaatgggta cttatgaaat aaaagctact attaattctt
    27721 catcaggtac tgcagataca actataagaa ttttttcaac aaaatttaca ggtgtaggta
    27781 acttcggcgg agtacctgcc ctttatccaa actgcatggt ctgccactcg ggaaatccgg
    27841 attttgctga tatttttgac agatggaaag tcagcgggca tgcaaatatt tttaaacaac
    27901 agatcgatag tggttcagca aattacaata caaattgtat gaaatgccac actaccggtt
    27961 acgatcacaa tcttgtaaat gaaaataatg gatttgatga cgtagcacgc agagtaggtt
    28021 gggaatggca gggaccacct gcaccgggaa aatgggacag tcttagaact cacttctcag
    28081 aactggctgc attttcaagt gtcggatgcg aaagctgtca cggggcagga ttttcacatg
    28141 catcaaatgg cggagatact aacaggattg atataaatta taaggctgat aactgcggaa
    28201 attgtcacgg tgaaccatgg agacacagta tattccagca atgggaaaat tctttgcact
    28261 ccgatccggt ttttgaagga agaaatgttt cagccagcca gagaaatact ctgagtgact
    28321 gtaacagatg tcatgacggt gaatcctatg ttgattttac aaagaaccgc ataggtacat
    28381 taaatcttac aactgcagat caggaatatg ttgcttgtca aacctgtcac gatcctcacg
    28441 gcaatagtaa tgaatatgat cttagaaatt tatcagcagg tgctgatact cttgcaaacg
    28501 gtacttcgta ttctactgcc ggaaatggga atgtatgtat gacctgtcat caggctcgaa
    28561 gaaataatca agtatatgta actaccggag gaaatgttaa tagtacttgg ggaccacacc
    28621 acagtactca ggcagatgtt ttgtttggca gtaatgctgc aacatttgga ttcccatata
    28681 ttacaggttc acacaaaaat attgaaaata catgtgcaac ctgtcacatg tcagcactga
    28741 cagatacagg tactgtaaca agagataagg tgggaggaca ctcaatgaat ttacattttg
    28801 aatcaacaga ttatgatcat actgctggtt gtgtttcttg tcacccggga gtcacatcat
    28861 tccgtgattt cattgctcct tcagatttcg acggaaacgg aattattgca ccatgggaag
    28921 atgaagtcga aggttgtctt acaaacctta gaatctctct gccaccaaca ggaattgatt
    28981 ctgtttcaac agctcttatc accgctgatt cctctaacct gaatctcaga aaagcatact
    29041 ggaattatct tttggtcact gaagaccaaa gcagcggata ccataatcca ttctttacaa
    29101 tacaggttct tctgacttcg agaaattata ctgtaggtat tgctcagtca ggaactgaag
    29161 ttcctgaggt ttatgaactt tctcagaact atccgaaccc gttcaatcca tcaacaaaaa
    29221 ttgactttgc aattccaaaa acaggaaagg tttcaataat aatttatgac attacaggaa
    29281 aagagattcg cgcactggta aatgacgaga ctatgtttgc aggtaaatat actgtggact
    29341 ggaattcatt aaataattca ggtcaacagg cttcaacagg tgtttacttc tacagattta
    29401 tctcagataa ttttgtcacg acaaagaaaa tgatgctgat caaataaaat taaattttaa
    29461 ataataaaca gtaaaaattt ataatacact tccggagctt tacagcttcg gaagtttttt
    29521 ttacttataa aattttcatt tatatttcat attttttaaa aataagaatc gttaaatttg
    29581 tcacgaattt aaattaaatt atgacagaaa ctatacaaaa accagcaggt ttgaaactct
    29641 ggcttatggc tgcaagagct tattcatttc ctgcttccat tattcctgtt ttacttggat
    29701 ccgtacttgc agttttatta aatccgggac ttcatttcaa cattctgaat ttcggactta
    29761 ctcttgtcgg atgcatactc gttcaggtag gtactaatct tattaatgat atttttgatt
    29821 ttgacaaagg cattgataaa gaagacaaag aactcggcat cccgcacggc ggctcaatgg
    29881 tgatttctct cggctttgtt acaataaaac aaatgaagac tgctgcattc ttttctcttc
    29941 tgatctcatt tcttattggt gtatatcttt ttacacaggc aggaatgtgg atactttatc
    30001 tgagcatttt cggtttgctg tcggctgtct tttatactgc aaagccactt gcgcttaaat
    30061 ataaagcact cggtgatctg caggtgataa tttcatttgg tgtcggaatg acactcggtt
    30121 cttacattgt acagacaggt gagttttcta taatgcctgt tatcatttca ataccacttg
    30181 gtcttttaat tgatgcaatc cttcattcaa ataatatacg tgatataaat ttcgacggta
    30241 agttcggtgt gaaaactctg ccaatactta ttggagaaaa actctctgta aagttttatt
    30301 actttctgat actcggcgca tatttagctg tgcttctttt tgtagtgtta aatattctgc
    30361 catggtttgc attactttca ttcataacct taccacttgc attaaaactt gtaaaaatgt
    30421 ctgaaggttt tccctcagat actcaggcaa gatttgagta cggtacaaaa catattatga
    30481 tgactgctca gcttaacatg atgtttgggc ttacacttgt tataggacta ttagtatcat
    30541 atttattttt cacttaattt ttttagggct ggaaattatt tcagcccttt ttttattccg
    30601 ttatttctct gtaaattttc cattcattcc cggtactgtc acttttataa attctcatta
    30661 ttttttcttc ggagtcattt aatttatcag taattaactc gtcgcggaat ttgatctttt
    30721 tatcatttgc agttacagaa gtatcattta tcactttaag atcccttata ttatactcta
    30781 atatattagt attcttaaaa atgctttcaa attttctgat cctttcattt ttccctgatc
    30841 tttttcctga tttagtataa aattcataat catcagtaag atagatcttc agagaatcgg
    30901 agtttcggtt ttcaattgcc aactcataat tttccattaa aatctggatt ctatatttat
    30961 ctgatgcaaa atattcattc agtctgttta tcgaaatata tacaagcagt ccaatcgcca
    31021 gtatcaataa taatatactg ctgtatgtaa ttatctgcct tcttcttgca aatgtgatct
    31081 tttcacgata tctttttttg tattcctttt tgaaatcatc cgatgctgca ctttttactt
    31141 caggagcttc attttcttta agattatatc cgcagtattt acaaaacaca gatgatgatt
    31201 caatcaactc actgcaggat gtacactgga tcagatcttc ctgtggaatg gtattcaaag
    31261 aatgtcggat ggattcaacg gtcagactgc ctacatcctc aggatcagaa ttttcacttc
    31321 tttctgtcct ttcagtcctt tcagaaagag gattattctg gcagccataa cttgtacatc
    31381 cattattttc atcccagcat tctttgtgat gaggcgtgcc gcactcagag cagaccgtaa
    31441 tgtctgcgcc tttttttaat ctcgactgac agtacggaca gatcttattc ttaaaatttt
    31501 catatttata tccacctgta ttcaaaaaac tggtctgcta attataaaaa atatcttatt
    31561 cagaaaatac atcttcatat tcaaattcaa tataaaattc cttctcacct tttcttaaaa
    31621 taattatatc attaaatgga agcttcatat cttcaaatgt tttatcagaa agccttttaa
    31681 acagttctga tcctgtattc aaactattaa aagaactgaa tttcaataaa ctgccgtctt
    31741 ccttttttac atcctttaca gacattagat ttacattccc aaaaaactca taagtcgtat
    31801 cagtattctc atctgtaagt tcatatacta tttcattatt aaattcgatc tgaaaattat
    31861 catccccgaa tagttttctt cctgattcat aaagatctga gatacctgca ctgctgaatt
    31921 ttttatcgat gtgaataata ttttcaaata cataatgcga aggacagtct tcattccttt
    31981 gatactctac aacatatgaa tcattgttcc caccgttaaa gacaaaccct ttaccggaaa
    32041 gcaaaagtaa gtcttctctt ttatgtaaat attttatagc ttcctgcacc tgtatcccac
    32101 cgatcacaga agcaatggta ggagttgtgg ggatcttacc ctgaatgatc tcatcaattc
    32161 caagcatcat acaggatttt ctcttgttaa ttattttata atctatctcc gacattgtac
    32221 attcataaca agcactgaat ggaggaatga aaattctcgc aacaccggtc agaacttcaa
    32281 tagctccgtc tatccacggg cgatttactt tccagcagga ttgatttata aaaagacgtg
    32341 cctctctgtt atcaagtcca ccaattatta tatcaaagtt tttaaagaca ccaagtccca
    32401 gattaaaaat gttgccgtta aaatatttaa tatttactga gtcgttgatc tctctcgcat
    32461 gtctgcaaat gacttcggct ttaggtttgc cttcatcttc aaatctgaaa agtacactcc
    32521 gggtaagatt gcttttttcc acattgtcca tatcagctac actgatattt ccgactccga
    32581 gcattgtcag atttttcaca atttcatttc cgagtgcgcc gcagcctgct acaagtactt
    32641 ttgcattttt aaggatgtcc tgatcccacc aagtgataag ctcaagacgg gaataccggt
    32701 cgtctaatat ttccaagatt aaaattaaat ttttgtaaaa tatctgaaat aataaataag
    32761 tgaaatgtta aaaaagaatt tattaagaaa gttctgaaag aaggtattaa gaatatttaa
    32821 taaaattcaa tctgataaat atttattata atatttaata tgatctgtgt aaaaggaata
    32881 cagtgtttta tccggctgtg atttccggat gaagtctcag tacgtcgcct tcttttacac
    32941 ctgcttcaag aagtgtctga ctttcagtaa gctgtttgcc tgaagctttg tgatgaaatt
    33001 tatatgaaag cggttccccg tccggacctg taagaggcat tttcattttt ggtaaaagta
    33061 cttccattat tctcttaacg gaagcatcat caggcagggt tgcctgctgt tctttgttgc
    33121 ctgttgcgtc aacaattgta atattaatac gtgccatagg tttgcaattt ataaataata
    33181 gttcattaat taaatgataa agttgaagca agtagaaata acaaatttta gaaatgtatt
    33241 ccaatttctg ttacattgac atttcccaaa gagtgatttc ataatatatt ataaaatgaa
    33301 tatacagctg tccaaaaaat ttagaaatga tctatatagt ttgaattatc tttgtgaatt
    33361 tggtatgtaa tttaatcaaa ctcttttaat attttgttaa tgcttacata gaatttccgg
    33421 caatttatca tcccgagtac ccgggacgat tattttgtta ctttttttgg tcgttcaaaa
    33481 aaagtaagca gaaaaaaata aatctttaat tttattcttt agcctttgta aagaatgtga
    33541 tttatatatg aaagtaaatt acacgacaca aaatcccgaa ctttccccaa accaaaaaaa
    33601 taatttcaa
//
LOCUS       DAORVQ010000011        33337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_407_length_33337_cov_6.694550, whole genome shotgun sequence.
ACCESSION   DAORVQ010000011 DAORVQ010000000
VERSION     DAORVQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 33337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33337
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_407_length_33337_cov_6.694550"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLX80_01980"
     CDS             complement(<1..184)
                     /locus_tag="PLX80_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19595.1"
                     /translation="MSDITLKENKISKEVLKKESYDFTDLPFWNTYYYSSGLLKKKME
                     ENIPLHLSKGKKYKVID"
     gene            complement(188..1657)
                     /locus_tag="PLX80_01985"
     CDS             complement(188..1657)
                     /locus_tag="PLX80_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY19596.1"
                     /translation="MLKDIKNTISQSAVYGLSRVATKLISFILIPLYTAKFSADAIAN
                     LNLLESFWQYLFTICMFAFETAIINFCTSEKNISKRNSIIFNFFFLTLINAAVFYVIS
                     LSFSGGISEIVLNENNSTVISYCFLISIFESLLIMPMTIARLNSRPGLYTIITISNLV
                     INLSLQLYFILGLGEGFEKVFLAKWIAPAIVFIVFIPYLIKNLSINIDFSEIKRILSF
                     SFPLMLAMLLSILLNSVDRFLLNDFVSKQQVAVYTTGYSIGSFTNAFIIAPFTLAINV
                     IFWNKINDDNFRRFMTKSSTYLFFVMILTSLVITYFIDYAVKIFVRNPELWSCVEIIP
                     IILFSNCFVALFIFSSLDFYYKKNTKTILLIMAICLSFNLIFNFIFIKYFGIYASAVI
                     TLLSYLLMTLIGAGLTKKFSFTKFEKAKVILLSVMFVLFSSYSYFVSIENIYLDISIK
                     LVLILLFLLLLHMFKFLEPIEIESLKGFVNKYFIKKLKH"
     gene            complement(1707..4109)
                     /locus_tag="PLX80_01990"
     CDS             complement(1707..4109)
                     /locus_tag="PLX80_01990"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HQY19597.1"
                     /translation="MKLITILFLILTVIVFFTGNVYSQTYEVSGMISDFKTNKPLEYV
                     TVKLADTTYGTTSEKNGEYFIRLKTGEYNLIFSYIGYFTDTTYILVEDKDISRNIFLS
                     PSEILTESIEVYGEDPAYEIIRRAISYKKEFKKTLNEYEYSAFSKFVIRSNQADVPPP
                     TVDGKPPDKDQLGIFGILESVTKGYFKKPDLQKQIVISKKETANITRGFALPLIINFY
                     DEEIDFNEFRIPTPLSDNAFDHYDYKLIGTTSIDSTLIYKIKVINRSESVPQLRGTVY
                     IADSIYSLMSVDMFNNDAAKPLGINKINFKQKFSPFEDSKNKNEKFWMPTDVQIFADG
                     SFLGLLKFEAEVFSIVSNYELNKKAPPGIFDDIVIKILPDAEKDSAYWAKNELLKNTP
                     EEKNAYKQIEIDDEIKSRTLNIGLNAISYGKKFSSNPVSYYLFNRVEGSALAFNLNYR
                     EPFRRRTAGGFFRYGFSDKKSKYELNYSQRFFNDRRLTLYGSAFQNTIIMAYRETIPF
                     QILDNTLKSLFDKKDNFDYFYSTGYDIGANYRFIPQLSALLEYRQAKQTTAYKNTNYS
                     IRKQDVEFINNPPINDAFLRTIDTKLIINPNKFRGIDWGDGDISRFRISNFPLFRIGF
                     TYSGKDLGSTYEYRKFYGNFGGKTYINAFMNIKYEIGGEIMTGQVPYQSLGYFNSRSG
                     TIDIDNAFMATDYNEFRGDQFYYLNFQNDFGKLLWGRIPVLNNFNLIGFFNAGRNFIS
                     AENYELAAFKNFTISDGIYMEAGFGINRILDIFRLDFAWRLNNPYKDNKRFYTNLSIG
                     VF"
     gene            complement(4106..4759)
                     /gene="rsmG"
                     /locus_tag="PLX80_01995"
     CDS             complement(4106..4759)
                     /gene="rsmG"
                     /locus_tag="PLX80_01995"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQY19598.1"
                     /translation="METLKKFISDELHSDPDFYLSNFEKYQDLILDWNKKINLISRKS
                     ESIEDHIVNSIFFLTKFKINNGQKIADIGTGGGFPGIPLKILYPEIELTLIDSIQKKV
                     TVLEDVIMKMNFKDTKAVWARAEELSMNKIYRNKYDIVISKAVATLDKLYLWGKNFLK
                     KDGKMICIKGGEINSEIETLNKLNEKINIEEILFSFGQGYNIEDKKIVVIQKKQTVK"
     gene            complement(4866..7259)
                     /locus_tag="PLX80_02000"
     CDS             complement(4866..7259)
                     /locus_tag="PLX80_02000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQY19599.1"
                     /translation="MRKYLLSILLVLTAPVLAFASEADLKIPELSADQNQLLIYGIVV
                     CILGIFFGIYQYLAVKKLPAHKSMLDVAQIIFETCKTYLLQQGKFLFILFIFIGACMT
                     FYFGALQQQSAGGVMLILMWTVIGILGSYGVAWFGIRMNTLANSRMAFASLERKPLKL
                     LTIPLNAGMSIGVLLICVELIMMLIIFLYVPREYAGASFIGFAIGESLGASALRIAGG
                     IFTKIADIGSDLMKVVFKIKEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVTGVA
                     LISFIVLAIGDIALQADLLVWLFVMRILMIITSIVSFYINGIVSKAKYSNKDELDFEA
                     PLTALVWITSIISIIVTFVASYYLIGDLPDDLWITLSYIISFGTLGAALIPEFTKIFT
                     SPKSKHVQEIVTASREGGASLTILSGLVAGNFSAFWKGMVFFGLMFGAYLVSNSIPEA
                     MMDYRAIFAFGLVAFGMLGMGPVTIAVDSYGPVTDNAQSIYELSLIEENKEEKEKEIE
                     RDFGFKPDWEKAKLYLEENDGAGNTFKATAKPVLIGTAVVGATTMIFSLILVLKNVLG
                     VEPETILNLLNPYSILGFLCGGSVIYWFTGASIQAVTTGAYSAVEYIKKNINLDDDAD
                     LKASTEKSKKVVEICTKYAQKGMFNIFIAVFSFALAFAFLSAPSGGDNAPASLFISYL
                     ISIAVFGLFQALFMANAGGAWDNAKKVVEVDLREKGTPLHDATVIGDTVGDPYKDTSS
                     VALNPIIKFTTLFGLLAMEIAITESFRDSAPIIGWIFFAIALYFVWRSFYKMRIIKN"
     gene            complement(7330..7965)
                     /gene="pth"
                     /locus_tag="PLX80_02005"
     CDS             complement(7330..7965)
                     /gene="pth"
                     /locus_tag="PLX80_02005"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQY19600.1"
                     /translation="MKLIVGLGNPGSKYTYTRHNIGFIVIDHFAESNGVLFMPGKGDW
                     YECSLTVNDEEVFLLKPVTFMNNSGSAVKEFCNIHNIKPEDVLAIYDDFQLPIGTVRV
                     RKKGSNGGHNGISDIIYQMNTEEIPRMRIGIGNNNVLQKDDFIDFVLSDFTKDEMNDF
                     IKIMPDLVSCIKSFITDEIKVTMNNFNKSFLQSTEIPKGNSENNADKKPDN"
     gene            complement(8002..8655)
                     /locus_tag="PLX80_02010"
     CDS             complement(8002..8655)
                     /locus_tag="PLX80_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQY19601.1"
                     /translation="MAEITLKALNRDTYKELTLNQLREKGIIPGIYYGHGVENISLAA
                     TEINLRPIIYTTESRIINLKFEDDRSFNCILKDVQFHPVSDKPLHFDLIAIKEGEMIT
                     IEVSVHITGNPPGVKEGGVLQHTLHKLQIQCLPKHIPSHIDVDVSELNVNDSIKIGDL
                     NIENITILNDENASVVAIVPPTVEKDPEEATEEPTEPEVISKSKKDEESDEDKKDKD"
     gene            complement(8872..8945)
                     /locus_tag="PLX80_02015"
     tRNA            complement(8872..8945)
                     /locus_tag="PLX80_02015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8909..8911),aa:Met,seq:cat)
     gene            9316..9684
                     /locus_tag="PLX80_02020"
     CDS             9316..9684
                     /locus_tag="PLX80_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQY19602.1"
                     /translation="MLESYIPIIIILAIAVIFGVVNVFFSSLFGPNRPNKEKLSTYES
                     GVEPIGTAHERFSVKFYMVAVLFILFDIEVVFLYPWAVSFINLDSIKMMYSFISVSVF
                     IVILLIGYIYVIKKGALKWD"
     gene            9675..10163
                     /locus_tag="PLX80_02025"
     CDS             9675..10163
                     /locus_tag="PLX80_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQY19603.1"
                     /translation="MGLEEKLSKDGFVTSKIDELIKWSRKNALWPMPMGISCCAIEMM
                     AAAGPRFDISRFGSEVFRFSPRQSDVMIVAGTTTYKMAQVVKKIYDQMPEPKWVIAMG
                     ACTSSGGMYRTYSVVQGIDLFLPVDVYVAGCPPRPDNLLKALMLLQEKISKGEEASFS
                     IN"
     gene            10194..11111
                     /locus_tag="PLX80_02030"
     CDS             10194..11111
                     /locus_tag="PLX80_02030"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY19604.1"
                     /translation="MKKIYAILFIIFFTAGVSTSQVIWNQGFESSDSANVPPGWSVWN
                     NSPADTLEPGWNWTVRPAGLNVPGLGTSLSVVHTGDKAIGVSWWTGQTSGICDAWLVT
                     QKVNNVPSDALLSFWVSGGSATFSDSVSVWVSTGDSTPASFLATPKGFRNQFFYPVGS
                     TYGLFEQQFVDLAPFAGQNIYIAFRYNMDVAVNGYFVYLDDIELTGTVGITQNGTSIP
                     AKFSLNQNYPNPFNPSTKISFDLAKSSNVNLVIFNSLGQKVMDVFQGQKPAGSYTATF
                     DGSSLSSGTYFYRLETESFTQTKKMQLIK"
     gene            11207..11689
                     /locus_tag="PLX80_02035"
     CDS             11207..11689
                     /locus_tag="PLX80_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQY19605.1"
                     /translation="MNTEITELLKSLLGNFQIEYTDVNGIPAIYLNEKEVLEVCKILK
                     NDESLKYNSLVDAVSVDRFEKKNRFEMIYNLVSIDFNRRLFIKIKLDSKNPEMETLSG
                     IWGSANWYEREAFDMMGIIFRNHPDLRRMYMPEEFEYFPLRKDFPLMGIPDSIPLPNK
                     "
     gene            11795..13072
                     /gene="nuoD"
                     /locus_tag="PLX80_02040"
     CDS             11795..13072
                     /gene="nuoD"
                     /locus_tag="PLX80_02040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HQY19606.1"
                     /translation="MSHTIYQDLKDGDTKIKKTKILEALESEDLSIQFSDALDNEMVL
                     NMGPQHPATHGVLRLLVSLDGETVMKCIPELGYLHRGYEKLAEASTYHEFIPHTDRLD
                     YLSPMANNVAYALAVEKLIDLEIPERGKYIRVIIAELARIQSHLLAIGALLMDVGAVT
                     PFLWAIREREKILDLYDIICGARFTTSYTRIGGVAQDVTDEALAGIKKFIDEFESNLG
                     EMRALVERNKIFIVRCENIGYLSKEDCIELGLTGPLTRAAGISRDLRKDNPYLVYDKL
                     DFNVPVYTDSDCLSRYYVRVDELIECVKILNQCIEKIPSGAHNALQSKKVLPQKERVY
                     TKMEELIHDFMIINFGVNPPKRESYSAIESSKGELGFYIISDGTGNPFRMKIRSPSFC
                     NLQALAPMMKKCLISDTVVIIGSVDPVMGEADK"
     gene            13173..13673
                     /locus_tag="PLX80_02045"
     CDS             13173..13673
                     /locus_tag="PLX80_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQY19607.1"
                     /translation="MSTENYTPIFDENEMKTVQLHISKYPKPMAAVMPVLWMIQDKYG
                     WISTESMRYVGELLSLPPDHVLGVATFYSMYFKKPMGKYHLQICTNVSCMLCDGYKIF
                     NHISEKLGIKNKEITPDGIFSIEEVECLGSCATAPMLQINNKEFHENLTLNKVDELLE
                     KLRNSN"
     gene            13689..15086
                     /gene="nuoF"
                     /locus_tag="PLX80_02050"
     CDS             13689..15086
                     /gene="nuoF"
                     /locus_tag="PLX80_02050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQY19608.1"
                     /translation="MEKIILKDIPDLDKIDVYLANGGYTAWKKVLKEMKPDEVIEEVK
                     TSGLRGRGGACFPTGMKWGFMPKGNEKPKFLCCNGDESEPGSFKDREIFEKNPHQFIE
                     GSLIAAYSMGIQAVYVYIRGEYWKWINIMEECVADAYKKGMIGKNIMDSDFSVEMYVH
                     KGAGAYICGEESSLMNSLEGKRGYPRVKPPFPASVGVWGNPTTINNIETLANVPEIIN
                     KGGEWFSKIGDPKHPGTLLYGVSGHVNKPGVYELPTGTPLMTIINDHCGGIKDGKDFK
                     MIIPGGSSMPPLTKEESLDMKMDAESLKAVGSAIGTAGIMVMDEDTDIVHVLQRITKF
                     YYHESCGQCTPCREGCGWMLKVLNRIMDGTGRHTDLDLLISVAENIEGNTICALGDAA
                     AWPVKWTVRKFRKEFEAKIKDDKIDLPVLNKVHGLRNSDEYLSVENETNQVVENLIGK
                     VQEVTQNEDVKRFSS"
     gene            complement(15208..17079)
                     /locus_tag="PLX80_02055"
     CDS             complement(15208..17079)
                     /locus_tag="PLX80_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQY19609.1"
                     /translation="MITLHKIFFRTVLLASIISYSFHPFNPFNPNDGKDDIEKLRNNN
                     LTSSEIYQHINYLSSDLLEGRFPGTEGDDMTTDYLSNEFKNYGLKPYGDEGYIQNFEM
                     YTHIKLSGENKFSTTSDGWDNAYVTGSDYTPLGFSDNGIVSGQLVFAGYGISAPDENY
                     DDYKNKDGKEIDIKGKVVVIMKYSPGGSNPHGNPFEKYEPLRQKAHIPKERGAAGIIF
                     INGPGTGDEDKLSDLKFDRVLQTAGLPVINCKREIIENLLNRNGMDLKKITEGIDESK
                     SSNSFEIKDGFAAIEVNIEPVKVITDNVIGLLEGNDPDLKNEVIVIGAHKDHLGYGLY
                     GSLYAGNDKQIHNGADDNASGTAGVLELAQKFSSEKIDLKRSILFICFGAEEAGLLGS
                     AYFTDSKLFEDLNVIAMINMDMVGRLSDDKLIIYGTGTSPVWDNILDAHNTDYNFKIT
                     KTPDGLGPSDHSSFYLKNVPALHFFTGTHTDYHSPPDDIEKINAEGELRILNFIYDIV
                     NDLDDANVKPEFTKVEISENNEKRSMGSVKIYVGTIPDYSYSGDGMKIAGVKEGGPAD
                     KGGMIAEDIIVKFGNVDVKNIYDYMFAMGEFKPGDEAEITVLRNNEPVVLKVTMGSR"
     gene            complement(17216..19366)
                     /locus_tag="PLX80_02060"
     CDS             complement(17216..19366)
                     /locus_tag="PLX80_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19610.1"
                     /translation="MKKIISLLFVAVLFLAMGNFANSQVDVTPSAATYANLKTAFNAI
                     NAGTHGNGAVTVTITANFAEPDSASLNGGVFTSCAINTNGAFTIAGNVNNAVVIFNGA
                     DNVTMDGRIGQAGSTRSLSITNTNVGTNNQIFKLQNGAMNDIVRYCNMTGVVNNGRMV
                     NIGQSNAGTGGNNGSVFEFNAITNGARSLQVFGTANGASSFTNDNTTFRGNLCKNATA
                     IGIFIGSLVNGVVCTQNEVFFDAPVMTTQASFTAIQVQGVGTISITKNKVYNFNLSGA
                     PNAAYRGSISLPASVAPLVDPATTVTYSNNMISIVTNSTNTAAAFKGACIQTQFSAAA
                     TSIGYTANVYNNTMFFGGSSSVLAADLSESYAGNVVLPIGAQSYALNYKNNIAVNKRT
                     GGSAAVFHIAQGYDSSSNLTVNADYNTAFASGSTGSWAVAYGAFVYPNGALEAWKSNT
                     CQLNFGTHDMFKDVAFVSASDLHLAAGRVGGDLNGTPIASVTDDIDGAAKNATYPYKG
                     CDEGAAFKILDMGAQLEGVLSTDQVIVYLQNAVCGTLSTSTADLNGTGKFAFGDSIAV
                     SSYTIMVDSRNHIRTASAGPVSIGAGTTVYNFRSAQSQAFGSNMILDGGVWSFFGGEV
                     TGDGVIDLADGAAVDNDASIFLSGCRVLTDVNNDGVVDLSDGVFPDNNAANFVAETLP
                     CPAPTSSFNTNSNTEKVQINTLDKSVINSEVTGN"
     gene            19546..20022
                     /locus_tag="PLX80_02065"
     CDS             19546..20022
                     /locus_tag="PLX80_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19611.1"
                     /translation="MNKFKLFSYRFLYLLCGIILIYMTITGSECQDALNAIVPEVPGD
                     IVGNWKLIEQTGSLQDICPDETVIFQTSGQAQLTCPNSNTITRNFEVVSNVLTYTQTS
                     ISYNMEYSNNNQELLLSGSNVDRNLKYQRVVTASDPVINAPLNKSNNFINSSDESR"
     gene            20026..21741
                     /locus_tag="PLX80_02070"
     CDS             20026..21741
                     /locus_tag="PLX80_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQY19612.1"
                     /translation="MKKLLFSPLIIIIVSFFIGSESSEVNKISSDGIKQITPTVKKVN
                     DVIREKKMSYNFTDVNVFEFNKNTGQNDNTSSFVNKATILKLNTSKLKDLIRLNKENI
                     EFRIPLTENENTVLELTRTQPESDDFKLIEKSGNGDKVVPYTQGLHYNGLIKGNENSL
                     ASLSIFENMVIALISDENGNYVLGSITNDDKSVSDNYIFYNDADMNIRNNFKCGVDGI
                     EEKFIRPIKVNENTVNNYQTDDPSRLPVKVYFEADNKMYQDNGNNTTNVGNFITAMFN
                     NVKTLYQNEQIPFEVSSVAVWTGNDPYSSLNDSYDVLLRFGANTQDNFQGNIAHLLST
                     RNAGLGGIAWIRVMCANYNAQDSAGRFAFSNIDNNYQSYPTFSWTVQVVTHEMGHSMG
                     SRHTHSCTWPVNGGFNRAIDSCYNAEGNCFNQTRARVGTIMSYCHLWTAAQGGGISFT
                     SGFGPLPGDTIRLRYAQAGCLGQVTNSSEQPSTFDLYQNAPNPFNPETKIKFDLPEEA
                     TVTLKVYDISGKTVAVLISGEFRLPGFYDVNFRASDYNLSSGIYFYELNTGTFREVKR
                     MVLIK"
     gene            21856..22266
                     /locus_tag="PLX80_02075"
     CDS             21856..22266
                     /locus_tag="PLX80_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19613.1"
                     /translation="MLMVIISASSCSSSEESDDFNFGKGKYRFKMSDSTGKALVSGII
                     EVKTYVNEDITGTLLVTKNYVDDFPGFTSMSNDFSGNINRVEKTVFINTNPKIADSNV
                     FWNMELGSNSVSGEWRYSIFRGNTTFGKIKITKY"
     gene            22389..23534
                     /locus_tag="PLX80_02080"
     CDS             22389..23534
                     /locus_tag="PLX80_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19614.1"
                     /translation="MKKKLTALTIVLPLLFIFFYADYSHSYPRFGAYTGNKCMDCHVN
                     PTGGLMRNKGGTYYAKKNLKMKMFKEIAGKTKFSPEITKNITLGGDVRVAQVDNEVDG
                     SNNFNSFLAMQGDIYFNAELNKILSVFATTGIYIPGVQTEYEVYGLISNLPANTYMKI
                     GRFAPNYGMRIVEHRAYQRQLILNTPYDANTGFELGVSPDWFNASVGLYNPMNASFLS
                     QDPHKMFTGSMDFTFGVENWDYTFNFGGSFLNNPFNTRDTTSTSIITALKQAYGAFGK
                     IGIMERVALLGELDFEESKSDRPLRRSLYWFGELDFIIIQGLELRGQYEYYDRNRDLE
                     GDDLKRISTGLGIFPFYGFETELMVRFVLEEPDQSNNEWQWNFHFYF"
     gene            complement(23566..23994)
                     /locus_tag="PLX80_02085"
     CDS             complement(23566..23994)
                     /locus_tag="PLX80_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQY19615.1"
                     /translation="MATIKVRNSEGYKSEIYSKSHMFFSDEPEDQGGTNEGADPYELL
                     LSALGSCKAVTMRMYAQRKDYPLNDIEIDLSNKKIHAEDCRDCETKDGKVDIIDVSIK
                     FTGDLSEEQISRLLEISKKCPVHKTLLSEIKINTNKEPAL"
     gene            24218..24934
                     /locus_tag="PLX80_02090"
     CDS             24218..24934
                     /locus_tag="PLX80_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQY19616.1"
                     /translation="MFLEGPKSRKREFIFLIEIFWEFIKGFRIFHFLGPCVTVFGSAR
                     FKEDHEYYKMARDIGARLVNIGFTVMTGGGPGIMEAANRGAKDAGGKSVGCNIELPFE
                     QDPNPYMDKWFTFKYFFIRKVLLLKYSYAFIVMPGGAGTMDELFETLTLIQTKKISDF
                     PVVIIGKAYWKNMLELLDDFVKNKTISPEDMKLFIVTDSVDEAIEHIEKFAIEKYKLV
                     KKNYSPVKIFGEKKSFQFPE"
     gene            complement(24938..26464)
                     /locus_tag="PLX80_02095"
     CDS             complement(24938..26464)
                     /locus_tag="PLX80_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQY19617.1"
                     /translation="MISEVYTATTYGVDAYIVRVESHIENGLFNFTIVGLPDNAVKES
                     RDRVSAAIKNSMFHFPVRRYTVNLAPADVRKEGSGFDLPIAVGLICETSQVSPVKVRD
                     YVFIGELSLDGKLRPVTGVLPISLEAKKNNYKGIILPTDNAKEAAIVEGIDVQPFDTL
                     TEVVDFLNDEITPKPFTIDVKKLFELEKNYLLDFADVKGQAEVKRAMEVAAAGAHNII
                     MIGLPGSGKTMVAKRLPTILPPLSLEEALETTKIHSIAGLLTSGTAIISTRPFRSPHH
                     TASDVSLVGGGPNAKPGEISFAHNGVLFLDELTEFKKNVLEVMRQPLEDRMVTISRSK
                     ITVEYPCNFMLVAAMNPTPAGTAKETDMYNDYDIQKFLSKISGPILDRIDLHIHVNPV
                     KYTELSSKTNLEKSEAVRCRVIAARNIQLERFKEKKGIYSNAGMTSKEIREYCKIDTE
                     CENLMKMAITKLGLSARAYDRILKVSRTIADLDASENILPAHISEAIQYRSLDRSGWV
                     "
     gene            complement(26534..27898)
                     /gene="der"
                     /locus_tag="PLX80_02100"
     CDS             complement(26534..27898)
                     /gene="der"
                     /locus_tag="PLX80_02100"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQY19618.1"
                     /translation="MENVVAIVGRPNVGKSTLFNRLVGKKTAIVHEQSGVTRDRNYGE
                     VEWTGKKFFLIDTGGYVPHSEEQFEKAIRAQVQLAIDEADEILFVVDAKNGLHPVDKV
                     LADMLRKHTETKKIILVANKIDIVERTSNALEFYSLGLGEPFAMSAINGFNSGDLLDI
                     ITADFPLASEEKEDNVIKFAIIGKPNAGKSSIANALLDEERNIVTDIPGTTRDTIDSN
                     MKYYGDDVLLIDTAGLRKKTKVKESIEFYSTIRAYKAIERCNVAILVIDATLLIEELD
                     KSSDMKLATFKLDKQDVKILEEVVRRRKGLLIVINKWDLVEKDANTAKIVEQKITEHL
                     KSFTYLKFVFISALTKQRIHKVLEEAVSIYKERAKKVKTSELNEALLNDIRATPPQSL
                     RGKEIKISYITQIKGSPPVFIFFVNDPEGINDNYKRFLEKKLREHFGFKGIPIDIAFR
                     KKND"
     gene            28211..29017
                     /locus_tag="PLX80_02105"
     CDS             28211..29017
                     /locus_tag="PLX80_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HQY19619.1"
                     /translation="MSKKLLFILLLCFMISSCGKDKSDTNTENSKETKAPKAEKVEFV
                     MKEYKKTYKDCDQNSEGCSYINFNYPEILNADIKDKVNSAIGTYLRDSIYSIEGKNFK
                     NFDELASTFFAEYDTMKRISSVQDLAFVLDEKAEIISETEKDFTVSISYYISTGGAHP
                     NSFMSYFVFDKLTGNIITLNNFFKPGYDSLLNKLVDGAYRKEKGLSPTDDLQKKGDLF
                     ENKITFNNNFAVTKDSLKFFYNNYEIAAYVFGPTEISIPLNDLKEYVKQE"
     gene            29142..29567
                     /locus_tag="PLX80_02110"
     CDS             29142..29567
                     /locus_tag="PLX80_02110"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HQY19620.1"
                     /translation="MNKNNPMEEVEKEVIKEIITESYEINEDDVTRIVNKEKEINKKT
                     SKLDLGKYMRFIKQIKLLLNLLKDYRNKAYTDVPWRSIALLSAAVLYFVNPFDVMPDI
                     IPLLGFADDSLLFAAVFKSVQTDLEKYAAWKGVKTENLF"
     gene            29604..30401
                     /locus_tag="PLX80_02115"
     CDS             29604..30401
                     /locus_tag="PLX80_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HQY19621.1"
                     /translation="MSDKIKVLFIGDIIGDPGYNLTKLMLPGYVTKYKTDFVIANGEN
                     ITEGKGILEKDGKKLLELDINVLTGGNHTMDKIQAHKYLMEEKRVLRPMNYPKGAYGH
                     GFGIYNIPGTDLKIGIINLQGRVFMKSIDCPFRTFDWVYEKIKYETNIVFVDFHAEAT
                     AEKIAFGWYANGRASVVVGTHTHIPTADSRVLSDGTAYITDVGMTGAYDSVIGMKKEG
                     SIKRFIYQTPQKYEVGEEDVRFSGVLCEIDSTSGKATSIKRLFSPEF"
     gene            30498..31157
                     /locus_tag="PLX80_02120"
     CDS             30498..31157
                     /locus_tag="PLX80_02120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HQY19622.1"
                     /translation="MKEMKSSPLNKEVYNYILDRFVPEDEITEAIIRETEELEIPLIQ
                     ISPDQGRFLYMLAKMLNAKNALEIGTLTGFSGYHIAKALPEGGKLITLELEKKHGEIA
                     EKYFEKAGLKDKTEVRISPAIDQLKILETENKKFDLIFIDADKVNYINYFEASLKLSH
                     SGTVMVFDNMTKDNRVVLDAGDDADLKAIQQTNDLLSLDKRVESFLLAIGDGFLITRV
                     K"
     gene            31538..32062
                     /locus_tag="PLX80_02125"
     CDS             31538..32062
                     /locus_tag="PLX80_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQY19623.1"
                     /translation="MKDIKNVLKNAVSFTKNEIQVIIFIAVVTAAGYSVKYYKSMTED
                     KNTAYDYSRSDSIFKERSLKIYAGNKKGISDKNSALDEPGMYKYFEIAEDSVKRSEKI
                     KADNEIPEFIIDINTAGIAELTELPGIGESIAGRIVEYRDKNKRFGNINEIMKVKGIG
                     KKKFEKLKKYIKAE"
     gene            32069..32956
                     /locus_tag="PLX80_02130"
     CDS             32069..32956
                     /locus_tag="PLX80_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19624.1"
                     /translation="MKKINIGFSGNNMRIMELTDTGEVNRLEEVTLSFNLDNDKLLKK
                     HRKEYLEEFSEIINGILDTSGLEPYEANILIDTGLAFMNIIPVDFSDRNSSINAHIHW
                     DLSNYFPETYKDYNVKCFRLNNYPVSEKVDEVLLIAVSNIKTDFLKNVCNNCNIIVKK
                     IEIDQFATEKYIRNKKSVAEASLIIGFRNNRLDLSLIVNGVLKYYDYEITEREIFKLP
                     LLRQLNFFLKRFGNIKIVEINIYGEEKVTDLKNFLIESVKNIPDLNVGNIDILYDTEI
                     HESKYAPLYGLAVSSNTTA"
     gene            32953..>33337
                     /locus_tag="PLX80_02135"
     CDS             32953..>33337
                     /locus_tag="PLX80_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQY19625.1"
                     /translation="MRIISGKYKGMKLSSFNEKNIRPTSDRAKETLFNILHNLMDFES
                     MKCLDLFCGSGALGLEMISRGADSCVFVDMNIDVAKKNAAYLYVDNKCEFERSDVLNY
                     LMKVKNEFDVVFCDPPYDYESYDDII"
BASE COUNT        11022 a         5711 c         5871 g        10733 t
ORIGIN      
        1 aatcgataac tttatatttt ttacctttgg aaagatgtaa aggaatattt tcttccatct
       61 tttttttcaa aagtcctgaa gaataataat aagtattcca gaatggcaga tcggtaaagt
      121 catatgactc ttttttgaga acttctttag aaattttatt ttcttttaaa gtaatatcag
      181 acaatattca gtgcttaagc tttttaataa aatacttatt tacaaatcct ttcagacttt
      241 caatttcaat tggttcaaga aatttaaaca tgtgaagaag tagcaaaaat aataatatta
      301 agacaagttt aatagatata tccaagtata tgttttcaat gctgacaaaa taggaatagg
      361 aagaaaataa gacaaacata actgaaagaa gaatcacttt tgctttttca aattttgtaa
      421 aagaaaactt ctttgtcagt ccagcgccga tcagcgtcat cagcagatat gataataatg
      481 tgatgacagc agatgcatag atcccaaaat attttatgaa aataaaatta aatataagat
      541 tgaagctgag acaaattgcc atgataagta atatcgtctt cgtatttttt ttataataaa
      601 aatcgagtga agagaatata aataatgcta caaagcaatt tgaaaaaaga ataatgggaa
      661 tgatctcgac acatgaccag agctcgggat ttcttacaaa gatcttaact gcatagtcta
      721 tgaaataagt tattacaagc gaagtaagta tcattacaaa aaataaatag gtcgaggact
      781 ttgtcataaa tcttctgaag ttgtcgtcat ttatcttgtt ccagaagatc acatttatcg
      841 cgagagtaaa cggtgcgatg ataaatgcat ttgtaaaact tccgatactg taaccagtgg
      901 tataaacagc aacctgctgt ttgctgacaa agtcattcag caaaaatcta tcaactgaat
      961 tcagtagaat agaaagcagc attgccagca tcagaggaaa tgagaatgac agaattcttt
     1021 ttatttcaga gaagtcaata tttatgctca ggttttttat cagataagga atgaaaacta
     1081 taaatactat cgcaggcgca atccattttg ctaaaaatac tttctcaaaa ccttccccga
     1141 gtccgagtat gaaatagagc tggagagaga ggtttatcac gagatttgaa attgtgataa
     1201 tggtataaag ccccggtctg ctgttaagtc tggcgatagt catcggcatg atcagcagag
     1261 actcaaagat cgagatcagg aaacaataag aaattactgt actgttattt tcatttaaaa
     1321 caatctcaga tatacctccg gagaaagaca gacttattac ataaaaaact gcagcattga
     1381 ttaatgtaag aaagaagaaa ttgaaaataa tgctgttacg tttggaaatg tttttttcgg
     1441 atgtacagaa attgattatg gcagtttcga aagcaaacat gcagatggta aaaaggtact
     1501 gccagaatga ttcgagtaag ttcagattag cgatggcatc tgctgagaac tttgctgtat
     1561 ataaaggtat gaggataaat gagatcagct tggtagcaac tctgctcagt ccgtaaacag
     1621 cggactgact tatcgtattt ttaatgtctt tcagcaatgt ctgaaaatat caactttaat
     1681 ctttatataa aatggaagaa gcgtttctaa aataccccga tcgaaaggtt tgtataaaat
     1741 cttttattat ctttataagg attattaagt ctccatgcaa agtctaatct gaaaatatca
     1801 agaatccgat ttattccaaa acctgcttcc atatatatac catcggagat cgtaaagttc
     1861 ttaaaggctg caagttcata attctcagca cttatgaaat ttctacctgc attaaaaaat
     1921 ccaatcagat taaaattgtt aagtactgga attctacccc ataacagttt accaaaatca
     1981 ttctgaaaat tcagatagta aaactgatca ccgcgaaatt cattataatc tgtcgccata
     2041 aaagcattat caatatcaat agtacccgat cgtgaattga aatatccgag cgactgatat
     2101 ggaacctgac cggtcatgat ctctccgcca atttcatact ttatattcat aaaagcattt
     2161 atataagtct tgcctccaaa atttccataa aattttctgt attcataagt actgccaaga
     2221 tcctttcctg aatatgtaaa cccaatccta aacaaaggga aattagagat acggaatctt
     2281 gaaatatctc cgtcacccca gtctattcct ctgaatttgt ttgggttaat aattaatttt
     2341 gtatctattg tccttaaaaa tgcatcattg atcggaggat tattaatgaa ttctacatcc
     2401 tgttttctta tgctgtaatt agtattttta tatgctgttg tctgctttgc ctgtctgtat
     2461 tccagtaatg cggacagctg aggaataaac ctgtaattgg ctcctatatc ataacctgta
     2521 gaatagaaat aatcgaaatt atctttttta tcaaacaatg attttaatgt attgtctaag
     2581 atctgaaagg gaattgtttc cctgtaagcc ataataattg tattttgaaa agctgatcca
     2641 tataatgtca atctcctgtc attaaaaaat ctctgtgaat agtttaattc gtattttgat
     2701 tttttatccg aaaaaccgta acggaaaaat cctccggctg ttcttcttct gaatggttct
     2761 ctgtaattca aattaaatgc cagtgcgctt ccttctaccc tgttgaataa ataatatgat
     2821 accggattgg atgagaattt cttaccgtaa ctaattgcat taagtccaat attcagtgtt
     2881 cttgatttta tttcatcatc tatttcaatt tgtttatatg catttttttc ttcaggagta
     2941 ttttttagaa gttcattttt tgcccagtaa gcagaatcct tttccgcatc aggtaaaatc
     3001 tttataacaa tgtcatcaaa tattcccggt ggtgcttttt tgtttaattc ataattagat
     3061 actattgaaa aaacttccgc ttcaaatttt aataaaccta aaaaagatcc gtcagcaaaa
     3121 atctgtacat cagtcggcat ccagaatttt tcattcttat ttttggaatc ttcaaacgga
     3181 gagaattttt gtttgaaatt tattttattt ataccaagcg gctttgcagc gtcgttatta
     3241 aacatatcca cactcataag agaataaatg ctgtctgcaa tataaaccgt gcctcttaac
     3301 tgaggtacac tttctgaccg gttgataact ttaattttgt agatgagtgt agagtcaatc
     3361 gaagtagtac caataagctt ataatcgtaa tgatcaaaag cattatcaga aagaggtgta
     3421 ggaattctga attcattgaa atcaatttcc tcgtcataaa aatttataat tagcggtaat
     3481 gcaaatcctc tcgtaatatt tgctgtttcc ttttttgaga tcacgatctg tttttgaaga
     3541 tcaggctttt tgaaatatcc tttggttaca gattcgagaa taccgaatat tccaagctga
     3601 tccttatccg gtggtttgcc gtcgacagtc ggaggaggta cgtcggcttg atttgagcgg
     3661 atcacaaatt ttgaaaatgc gctgtactca tattcattaa gagttttttt gaattctttt
     3721 ttataactga tcgccctgcg gataatttca tatgcaggat cttcaccgta tacttcaata
     3781 gactctgtaa gtatttcaga cggtgataaa aaaatatttc ttgaaatatc tttatcctct
     3841 actagtatat aagttgtatc agtaaaatat ccgatataag aaaaaattaa attgtattct
     3901 cccgtcttaa gtcttataaa atactctccg ttcttttctg atgtggtgcc gtaagtcgta
     3961 tctgctaatt ttacagttac atattcaagc ggcttattgg ttttaaaatc tgatatcata
     4021 ccgcttactt catatgtctg tgaatataca tttcctgtaa aaaacacaat aacggtaagt
     4081 attaaaaaaa gtatagtgat taatttcatt taacggtttg ctttttttga ataacaacaa
     4141 tttttttatc ttcaatatta taaccctgac caaagctgaa taaaatttcc tctatattaa
     4201 ttttttcatt taatttattc aaggtttcaa tttcactatt tatttcaccg ccttttatac
     4261 agatcatttt tccgtctttc tttaaaaaat ttttacccca gagatatagt ttatcaagtg
     4321 ttgctactgc ttttgaaatt acaatatcat atttgtttct ataaatttta ttcatcgaaa
     4381 gctcttcagc tcttgcccat acggctttag tatctttgaa gttcattttc ataatgacat
     4441 cttccagtac agttactttt ttttgaatag aatctattaa ggtaagttct atttcaggat
     4501 atagaatttt cagaggtatt ccagggaatc cacctcctgt gcctatgtca gcaatctttt
     4561 gaccgttatt aattttgaat tttgtaagaa aaaaaattga atttacgata tgatcttcaa
     4621 tagattcaga ttttcttgaa attaaattta tctttttatt ccagtcgagt atgaggtcct
     4681 gatatttttc aaaattactt aaataaaaat ccggatctga atgaagttcg tctgagataa
     4741 atttctttag agtttccaag atagaaaaat ttttatttgg aaaattgaca tgcttaatta
     4801 atataaaaaa ggcatcctgt tatggatgcc tttttgaaat gctaaaatat tatttattaa
     4861 aatggtcagt ttttaattat tctcatttta tagaaagatc tccatacaaa atataatgca
     4921 attgcaaaga atatccatcc gatgatcggt gctgaatctc tgaaactttc tgtgattgca
     4981 atctccatag caagtagacc aaacaatgtt gtgaatttaa tgattggatt cagcgcaaca
     5041 gaagaagtgt ccttgtaagg atctccgact gtatctccaa ttacggttgc atcatgcaga
     5101 ggagttcctt tttctcttag atccacttcc actactttct tagcattatc ccaagcacca
     5161 ccggcgttag ccatgaagag agcctgaaac aatccgaaca ctgcaatcga gatcagataa
     5221 cttataaata atgaagccgg agcattgtct cctccagacg gagccgataa gaatgcaaat
     5281 gcaagtgcga aagaaaatac tgctatgaag atattgaaca ttcctttctg agcatacttt
     5341 gtacagatct caacaacctt ttttgatttt tctgtcgaag cttttaaatc tgcatcatca
     5401 tcaagattaa tatttttctt aatatattca accgcgctgt aagcacctgt tgtaactgcc
     5461 tgtatagaag cacctgtaaa ccagtatatc accgaacctc cgcaaaggaa tcctaaaatt
     5521 gaatatggat tcagcaggtt aagaattgtc tcaggttcga caccgagtac atttttcaaa
     5581 acaagtatca gagagaatat catagttgtc gcgccgacaa cagctgttcc gataagaacc
     5641 ggtttagcag ttgccttgaa tgtatttcca gcgccgtcat tttcctcaag ataaagttta
     5701 gctttttccc agtcaggctt aaatccgaaa tctctttcaa tttctttttc tttctcttct
     5761 ttattttctt cgataagaga taattcatat atcgactgag cattgtcagt aaccggaccg
     5821 tatgagtcca cagcaatagt tacaggaccc attccgagca taccaaaagc cacaagacca
     5881 aaagcaaata tcgctctgta gtccatcatt gcttcaggga ttgaattgga tacgagatac
     5941 gcaccaaaca tcaagccgaa gaaaaccata cctttccaga atgcagaaaa attacctgct
     6001 acaagtcctg acagaatagt aagggaagct ccgccttctc tggatgcagt tacaatttcc
     6061 tgtacatgct tagatttagg cgaagtaaat atttttgtga attcaggtat aagtgctgca
     6121 cctaaagttc caaaactgat tatataagat aatgttatcc aaagatcatc gggaagatca
     6181 cctatcagat aataagaagc tacgaatgtt acaataatag aaattataga tgtgatccag
     6241 acaagtgcag tgagaggagc ttcaaaatcc agctcatctt tatttgaata ttttgcctta
     6301 gatacaattc cgttaatata gaatgaaaca attgaagtaa tgatcattag aattctcatt
     6361 acaaataacc aaaccagcag atctgcctgc aaagcaatat caccgattgc cagtactata
     6421 aaactgataa gtgcaacacc ggtaacaccg taagtttcaa aaccgtcagc tgttggacct
     6481 acagaatcac ctgcattatc accggtacag tctgcaataa caccggggtt tcttggatcg
     6541 tcttctttaa ttttaaaaac gactttcatc agatccgaac cgatatctgc gatctttgta
     6601 aaaatacctc ctgcaattct taaagcggaa gcacctaaag attcaccgat tgcaaatcca
     6661 ataaaactag ctcctgcata ttctcttgga acatacagaa aaatgatcaa catcataatt
     6721 aattcgacac agatcagaag tacaccaatg ctcatccctg cattaagcgg gatagtaaga
     6781 agttttaaag gttttctttc aagtgatgca aaagccattc tgctgtttgc aagtgtgttc
     6841 attctgatac caaaccatgc tacaccataa gagccaagta taccgataac agtccacata
     6901 agaatcagca ttacaccgcc cgcagactgt tgttgcaatg caccgaagta aaatgtcata
     6961 catgctccga taaatataaa cagtatgaac aggaattttc cttgctgcaa gagataggtt
     7021 ttacatgttt caaaaattat ctgtgctaca tcaagcattg atttatgcgc aggtaatttt
     7081 tttactgcca aatactgata tatcccgaag aatatcccca ggatacaaac tacaattcca
     7141 tagatcagca actgattttg gtcagcgctt agttcaggaa ttttaaggtc agcctcactt
     7201 gcaaaagcca gcacaggtgc tgtaagtaca agcaggatac ttaataaata tttcctcatt
     7261 ttttaaatta tgattttctg attatgttta aaaagttctt attgataagt tgtaagttct
     7321 atattgccgt taattgtccg gctttttatc agcgttattt tcagaatttc ctttcgggat
     7381 ttcagtactt tgtaaaaaag atttattaaa gttattcatt gtgactttaa tttcatcagt
     7441 aataaaactt tttatacaac ttaccagatc aggcattatc ttaatgaaat cattcatttc
     7501 atctttagta aaatcactta aaacaaaatc aataaaatca tctttttgta agacattgtt
     7561 atttccaata cctatcctca ttctaggaat ctcttcagta ttcatttgat aaattatatc
     7621 tgaaataccg ttgtgtcctc cgttacttcc tttctttctt actcttacag taccaatcgg
     7681 taattgaaaa tcatcataga tcgcaagtac atcttcaggt tttatattat gtatattgca
     7741 gaattcctta actgcagaac cactgttatt cataaatgta actggtttaa gaagaaaaac
     7801 ttcttcatca ttaacagtca gtgagcattc ataccaatcg cctttgcctg gcataaacaa
     7861 aacaccgttt gattcagcga aatggtctat tacaataaat ccaatattat gtcttgtgta
     7921 tgtatatttt gaacccggat ttcctaatcc aactattaat ttcacaatga gaaattttta
     7981 aaccatgtga aataaagatt actaatcttt atccttttta tcttcgtcag attcttcgtc
     8041 tttcttagat ttggaaatta cttccggttc tgtaggctct tcagtggctt cttccggatc
     8101 tttttcaact gtcggaggaa caatagctac aacggatgca ttttcgtcat ttaatattgt
     8161 aatattttca atattaagat caccgatttt tattgagtca tttacattta actctgatac
     8221 atctacgtca atatgtgatg gaatatgttt tggaagacac tgaatctgta atttatgaag
     8281 tgtatgctgt aaaactccgc cctctttaac accgggtgga tttcctgtga tatggactga
     8341 cacttcgatt gtgatcatct ctccttcttt aattgcaatg agatcaaaat gaagaggctt
     8401 gtcagataca ggatgaaact gaacatcctt caggatacag ttaaaacttc tgtcatcttc
     8461 aaatttcaga tttatgatcc tggattcagt agtatagatt attggtctca ggtttatttc
     8521 ggtagctgcc aaactaatgt tttcaactcc atgtccatag taaatgcccg gaattatacc
     8581 tttttctcta agctgattaa gagttaactc tttataagta tctctgttca gggcttttaa
     8641 tgttatttct gccattataa atttttttaa aagtgaacta caaaaatagc ttttatataa
     8701 aaattaatca attgaaatat tttatacaga taactaatga tatattttga aaattgaaaa
     8761 agttaagtga aaaatttaaa atcaggaaag aatttatcaa cattaaaaca gtatcagggg
     8821 aattaatatt gaatagagga aaattaattt agtcttaaaa aaaattaggt gtaccgggag
     8881 agggacttga acccccgacc tgcggattat gattccgacg ctctaaccag ctgagctacc
     8941 ccggcaaaat ttcaaacgag taagttaact ttattgaact tcttattcaa tgatatttta
     9001 aggtaactct gtcaaatctt tttttattta aaataaaatt agtatgtaat tcattggaga
     9061 taactttaat aacggaagtt tcgaataata atttttcaaa ttaatacttc tgaaagttat
     9121 tttatgaaaa agagtttcaa ctgaaatttg tatttcgaat agagttttga aaagatcatc
     9181 atgctgttta caataattat ctatacttga gttagttcaa tttatttaaa atgttataat
     9241 ttaacattta tatcaataaa ttaattctaa aattttttcc attaaaatta aaaaaaaaaa
     9301 attttatatt gaccaatgtt agaatcgtat ataccaatta ttataatact tgcaattgca
     9361 gttatatttg gagttgtcaa tgtatttttt tcatctttat tcggtccaaa ccgaccaaac
     9421 aaagaaaagc tttctactta tgaaagcggt gtagagccta tcggtactgc acatgaacgg
     9481 ttttctgtaa agttttatat ggtagctgtt ctttttatat tatttgatat agaggtagtt
     9541 tttttatatc cgtgggcagt atcttttata aatcttgaca gtataaaaat gatgtattca
     9601 tttatcagtg tttcagtatt tattgtcata ttgttgatcg gatatatata tgtaattaaa
     9661 aaaggagctt taaaatggga ttagaagaga aattatcaaa agacggattc gtcacttcaa
     9721 aaatagacga acttataaaa tggtcaagaa aaaatgcttt gtggccgatg ccaatgggta
     9781 tttcttgctg cgcaattgaa atgatggctg cagccggacc aagatttgat atatcaagat
     9841 tcggaagtga agtattcaga ttttcaccaa ggcaatctga tgttatgata gtagccggta
     9901 ctactactta taaaatggca caggttgtaa aaaaaattta tgatcagatg ccggaaccaa
     9961 aatgggttat tgctatgggt gcatgcacat caagcggagg aatgtacaga acatattctg
    10021 tagttcaggg aattgatctt tttttacctg ttgatgttta tgtagcaggt tgtcctccaa
    10081 gacccgacaa tctcctgaaa gcacttatgt tacttcagga aaaaatttca aaaggggaag
    10141 aagcaagttt ttcaataaat taaaattttt ttataaacct aaataaaaat atcatgaaaa
    10201 aaatctacgc aattttattt attatctttt ttactgcagg agtttcaact tctcaggtaa
    10261 tctggaatca gggttttgaa tccagtgatt ctgctaatgt acctccgggc tggtcagtct
    10321 ggaataattc accggccgat acattagagc cgggatggaa ctggacagta agacctgcag
    10381 gacttaatgt tcccggatta ggcacttctt tatcagtagt ccataccgga gataaagcaa
    10441 tcggagtaag ttggtggact ggccaaacat caggaatctg cgatgcatgg ttagttacac
    10501 aaaaagttaa caacgttcct tctgatgctt tgcttagttt ctgggtatca ggcggatctg
    10561 ctacatttag cgacagtgtt tctgtatggg taagtacagg tgattccaca cctgcttcat
    10621 tccttgcaac tccaaaggga ttcagaaatc agttctttta tccggtagga tcaacatacg
    10681 gattatttga acagcaattt gttgatcttg ctccatttgc aggacaaaat atttatatag
    10741 cattcagata taatatggat gtagctgtaa acggatattt tgtatatctg gatgatattg
    10801 agttaacagg aacagtaggt attacacaaa atggaacaag cattcctgca aagttttcat
    10861 taaatcaaaa ttatccaaat ccgtttaacc caagtacaaa aatttctttt gatcttgcaa
    10921 agagttcgaa tgtaaatctt gtaattttca attcattagg tcagaaagtt atggatgtat
    10981 ttcaaggtca gaagcctgca ggttcttata ctgcaacatt tgacggatca tcactttcaa
    11041 gcggaacata tttctacaga cttgaaactg aatcctttac acagacaaaa aaaatgcagt
    11101 tgataaaata aaaaacaaca attatatata ttcaaagaac ccgcattagc gggttttttg
    11161 ttatttaaaa tgaaaatatt attaagtaag tttgttaaat aaatttatga atacagaaat
    11221 taccgaatta ctgaaatccc ttctgggaaa ttttcaaata gaatatactg acgtcaacgg
    11281 aattcctgca atttacctaa acgaaaaaga agttttggaa gtatgtaaaa ttctaaagaa
    11341 tgatgaatca ttaaaatata attctttagt ggatgctgtc agtgttgaca ggtttgaaaa
    11401 gaagaacagg tttgaaatga tttacaacct tgtttcaatt gattttaaca gaagattatt
    11461 tatcaagatc aaattagatt caaaaaatcc agagatggaa acacttagcg gaatttgggg
    11521 atctgcaaac tggtacgaga gagaagcttt tgacatgatg ggaataatat tcagaaatca
    11581 tccggatctc agaaggatgt acatgccgga agagtttgaa tattttccgt tgagaaagga
    11641 ttttccgctg atgggtatcc cggattcaat tccactgcca aataaataat agattttata
    11701 tcagttaatt ttaaattgat taaaaaaaat atactgttaa ataaaatttt ttttcttctt
    11761 cagtaataat aattataaac acaaactgaa aaatatgtct catactatat atcaggatct
    11821 taaagatggt gatactaaaa tcaagaaaac aaaaattctc gaagcacttg agtcagaaga
    11881 tctgagtatt cagttttctg atgctttaga taatgaaatg gttctgaata tggggcctca
    11941 gcatccggcc actcacggag tactgagact gcttgtcagt cttgacggtg agactgttat
    12001 gaaatgtatt cctgaacttg gctatcttca caggggatat gaaaagcttg ccgaagcaag
    12061 tacttatcac gaattcattc cacacactga cagactcgat tatctttctc caatggctaa
    12121 caatgttgca tatgctcttg cagtcgaaaa attgatcgat ctagaaattc ctgaaagagg
    12181 taaatatatc agagttataa ttgctgaact tgcaagaatc cagtctcatc tgcttgcaat
    12241 aggtgcattg ctgatggatg tcggcgcagt aactcctttc ttatgggcaa taagagaaag
    12301 agaaaaaata ctggatctct atgatataat atgcggtgcg agatttacaa cttcctatac
    12361 gagaattggc ggagtagcac aggatgtaac tgatgaagca ttagccggga taaaaaaatt
    12421 catagatgaa tttgaaagta atcttggtga aatgagagca ctcgtcgaaa ggaacaaaat
    12481 ttttatagtc agatgtgaaa acatcggata tctttctaaa gaagattgca tagagctggg
    12541 acttacagga cctttgacaa gagctgcagg tatcagcagg gatctgagaa aagacaatcc
    12601 gtatcttgtt tatgataagc ttgatttcaa tgtgcctgtt tatacggaca gtgattgtct
    12661 ctcaagatat tatgtgagag tggatgaact catagaatgt gtgaaaattt taaatcagtg
    12721 tatcgaaaaa ataccttccg gtgcacacaa tgcccttcag tcaaaaaagg tattgccgca
    12781 gaaggaaaga gtttatacta agatggaaga gcttattcat gattttatga taattaattt
    12841 cggagtaaat cctccaaaga gagaatcata ttcagcaata gaatcttcaa aaggggagct
    12901 tggattttat ataataagtg acggtacagg taatcctttc aggatgaaga ttcggtctcc
    12961 gagtttttgt aatctgcagg cacttgcacc gatgatgaaa aaatgtctga tctctgacac
    13021 ggtggtaata ataggaagcg tggatcctgt gatgggcgaa gctgataagt aattttaatt
    13081 ttacacttta ttcagaatat attaataagt taataattag taacgatcaa tttatttctg
    13141 atgatatata ataaatattt caaacaaatt ttatgagcac agaaaattat acaccgatct
    13201 ttgacgaaaa tgaaatgaag acagtacaac tacatatttc aaaatatcca aaacccatgg
    13261 cagcagtaat gcccgtcctc tggatgattc aggataaata cggatggata tccactgagt
    13321 caatgagata tgtgggagag ctgctgagtc ttccgccgga tcacgtactt ggagttgcga
    13381 ctttttattc aatgtatttt aaaaagccaa tggggaaata tcatttacag atctgcacaa
    13441 acgtttcatg tatgttgtgt gacggatata aaatttttaa tcatatttca gaaaagctcg
    13501 gtattaagaa taaagaaatt acacctgatg gaatattttc aatcgaagaa gtagaatgtc
    13561 tcggaagctg tgcaactgca ccaatgcttc agatcaacaa taaagaattt catgaaaatc
    13621 ttactctgaa caaagtagat gagcttcttg agaaactaag aaattcaaat taattacaat
    13681 ttatttcaat ggaaaagata attttaaaag atataccgga tctcgataag attgatgttt
    13741 atttagctaa tggcggatat actgcatgga aaaaagtttt gaaggaaatg aaaccggatg
    13801 aagtgatcga agaagtaaaa acttcaggtc tcagaggaag aggcggcgcc tgtttcccta
    13861 ccggtatgaa gtggggtttt atgcctaaag gaaatgagaa gccaaaattc ctttgttgca
    13921 acggtgatga aagtgaaccc ggcagtttta aagacagaga aatttttgaa aaaaacccac
    13981 atcagtttat agaaggctct ctgatagctg catattcaat gggaattcag gcagtgtatg
    14041 tttatatcag aggtgaatac tggaaatgga ttaacattat ggaagagtgt gtagctgatg
    14101 cttataaaaa gggaatgatt ggaaaaaata ttatggattc agatttttca gtagaaatgt
    14161 atgtacataa aggagcagga gcttatatct gcggagaaga atcttcgctt atgaattcac
    14221 tcgaaggtaa gcgcggatat ccaagagtca aaccaccatt ccctgcatct gtaggtgtct
    14281 ggggaaatcc gactacaatc aataatattg aaactcttgc aaatgtaccc gagattataa
    14341 acaaaggcgg cgaatggttc agcaagatcg gtgatccgaa acatccggga actctgcttt
    14401 acggagtcag tggtcatgta aataaaccgg gcgtatatga acttccgact ggcactccgc
    14461 ttatgacaat tataaatgac cattgcggtg gaataaagga cgggaaagat tttaaaatga
    14521 tcatacccgg cggatcttca atgcctcctc ttacaaagga agaatctctc gatatgaaaa
    14581 tggatgccga aagtctgaaa gctgtcggct cagcaatcgg tactgcaggc attatggtaa
    14641 tggatgaaga tactgatatt gtacacgtac ttcagagaat cacaaagttt tattatcatg
    14701 aatcatgcgg acaatgtact ccctgtcgtg aaggatgcgg atggatgttg aaagttctca
    14761 acagaataat ggacggcaca ggaagacata ccgatctgga tctgctcatt tcagttgcag
    14821 agaatataga aggaaatacg atctgcgcgc ttggagatgc agcagcatgg cctgtaaaat
    14881 ggactgtaag aaaattcaga aaagaatttg aagcaaaaat aaaagatgat aaaattgatc
    14941 tgccggtctt gaataaagtt cacgggctga gaaattctga tgaatattta tctgtagaaa
    15001 atgaaaccaa ccaggtagtt gaaaacttaa ttggtaaagt tcaggaagtt actcaaaacg
    15061 aagacgtaaa acgctttagc tcctaacaat attaaatttt aaataataaa tagttaatag
    15121 ttaatagtta atagttaata gttaatagtt aatagttatc tgaaattttt aattcttaat
    15181 tcttaattct taatttttaa ttgctcttta cctgcttccc atcgtcacct tcaaaaccac
    15241 aggttcatta tttctcaata cagtaatttc agcttcatca cccggtttaa attctcccat
    15301 tgcaaacata tagtcgtaaa tattttttac atctacattt ccaaatttta caattatatc
    15361 ttcagctatc attccgcctt tgtctgcagg accaccttct tttacaccag ctattttcat
    15421 cccatctcca gaatacgaat agtcagggat cgtgcctaca tagatcttta cgctgcccat
    15481 agatctcttc tcattgtttt ccgaaatttc aactttagtg aattcaggtt ttacatttgc
    15541 atcatccagg tcattcacta tatcatatat aaaatttaaa attcgaagtt cgccttcagc
    15601 atttattttt tcaatatcat ccggaggtga atgataatct gtatgagtgc ctgtaaaaaa
    15661 atgaagtgca ggtacattct ttaaataaaa actcgaatga tcagaaggac cgagtccgtc
    15721 aggggtcttt gttattttga aattataatc agtattatgt gcgtccagta tattatccca
    15781 gacaggtgaa gtacctgttc cataaataat tagtttgtca tcagaaagtc ttccaaccat
    15841 atccatattt atcattgcta ttacattcag atcttcaaat aattttgaat ctgtaaaata
    15901 tgctgatccg agaagtccgg cttcctcagc tccaaaacag ataaacagaa tacttctttt
    15961 cagatcaatt ttttcggaag aaaatttctg agcaagttca agtactcctg ctgttcctga
    16021 tgcattatca tctgctccat tatgtatctg cttatcgttt cctgcataaa gtgaaccata
    16081 caacccgtat cccagatgat ctttgtgcgc cccaattact atcacttcat tttttagatc
    16141 aggatcattt ccttcaagta aaccgatgac attatcagtg ataactttaa caggttcgat
    16201 attaacttca attgcagcaa agccgtcttt aatttcaaat gaatttgatg atttactttc
    16261 gtcaattcct tctgttattt ttttcagatc cattccgttt ctgttcaaaa gattttcaat
    16321 aatctctctc ttacaattta ttacaggcaa tcccgcggtc tgaagtactc tgtcaaattt
    16381 caaatctgaa agtttatctt catccccagt cccgggtccg ttaataaaaa taattcctgc
    16441 cgctcctctc tctttaggaa tgtgagcctt ctgtcttaat ggttcatatt tctcaaacgg
    16501 attaccgtga ggattgcttc cgccgggaga atatttcata atcacaacta ctttaccttt
    16561 tatgtcaatt tcttttccat ctttattttt atagtcgtca taattttcat caggtgcaga
    16621 aattccatac cctgcaaata ctagctgacc cgatacaatt ccgttgtcag aaaatccaag
    16681 cggtgtataa tccgatccgg ttacatatgc attatcccat ccgtctgaag tagtagaaaa
    16741 tttattttca cctgaaagct ttatatgagt gtacatttca aaattctgaa tgtagccttc
    16801 atcgccgtaa ggtttcagac cgtaattttt aaattcattg gacaaataat ctgttgtcat
    16861 atcatcacct tctgtacccg ggaatctacc ttcaagtaaa tctgaagaca aataatttat
    16921 atgctgatat atttctgatg aagtaagatt attatttcgt aatttttcta tgtcatcctt
    16981 tccgtcattt gggttaaaag gattaaaggg atgaaaggaa tatgagataa ttgaagcaag
    17041 caaaactgtc ctaaagaaaa ttttgtgtaa tgtaatcata tttttttttt gtaatactaa
    17101 attttatata tcagataata aaatcaagat aataattaaa tctaaatctg atcataaaat
    17161 aaaaaacccc ggtagttaaa aaccaccggg gtaataaatt aatatgtttc agattttagt
    17221 tgcctgtaac ttctgaatta ataacgcttt tgtctaaagt atttatttgt actttttctg
    17281 tatttgagtt tgtattaaat gaggatgtag gtgcaggaca tggtaaagtc tctgcaacaa
    17341 agtttgcagc attattgtcc gggaatacac catcagataa gtctacaact ccgtcattat
    17401 tgacgtcagt taaaactctg catcctgaaa ggaaaatgga tgcatcatta tcaacagcag
    17461 ctccgtcagc aaggtcaata actccgtcac ctgtgacttc accaccaaag aatgaccaaa
    17521 caccaccatc taagatcata ttactgccaa atgcctgtga ctgtgcagat ctgaaattat
    17581 aaactgtggt acctgctccg attgatacag gacctgcact ggctgttctg atatgatttc
    17641 tgctgtccac cattattgtg taagatgaaa ctgcaataga atcaccaaat gcaaattttc
    17701 cggtgccgtt aagatctgct gtacttgttg ataaagttcc acaaacagca ttttgtaaat
    17761 aaactataac ctgatctgta cttaatacac cttctaactg tgcacccata tcaaggatct
    17821 tgaatgcagc accttcgtca catcctttgt aaggatatgt tgcgtttttt gcagcaccgt
    17881 caatatcatc agtaactgat gcgattggtg ttccgttaag atcgccgccg actctgcctg
    17941 cagctaaatg aagatctgaa gctgatacaa aagctacatc tttaaacatg tcatgagttc
    18001 caaaattaag ctgacaagta tttgacttcc atgcttctaa tgctccgttt ggatatacaa
    18061 atgctccgta tgcaactgcc catgatcctg tagatccgct tgcaaatgca gtattgtaat
    18121 ccgcatttac agtcaggttt gaagaactgt catatccctg tgcaatatgg aatactgctg
    18181 cagaaccacc ggttcttttg tttactgcaa tgttgttttt ataatttaaa gcataacttt
    18241 gtgctccgat aggaagtaca acgtttccag catatgattc agataaatct gcagctaaaa
    18301 ctgaagaaga acctccgaag aacatagtgt tgttatatac atttgctgtg tatccaattg
    18361 aagtagctgc tgctgaaaac tgtgtctgaa tacatgcacc tttaaaagct gcagctgtat
    18421 ttgtagagtt agtaacaatt gagatcatgt tgtttgaata tgtgacggtc gttgctggat
    18481 ctactaatgg tgcaactgaa gcaggtaatg agatcgatcc tctgtaagca gcgtttggtg
    18541 ctcctgaaag attaaagtta taaaccttat tttttgttat tgaaattgtt cctactccct
    18601 gaacctgaat agctgtaaat gaagcttgtg ttgtcattac tggtgcatca aaaaacactt
    18661 cattttgagt acatacaaca ccgtttacta atgatccgat gaaaatacca atagctgtag
    18721 catttttaca taggttacct ctgaaagttg tattatcatt tgtaaatgaa ctagcaccgt
    18781 ttgctgtacc aaatacctga agacttcttg ctccattggt aatggcgttg aattcgaata
    18841 cactaccatt gttaccaccg gtacctgcat tggactgacc aatgttaacc attctaccgt
    18901 tattaactac accggtcata ttacaatatc ttactatgtc gttcatagca ccgttttgta
    18961 atttgaaaat ctgattattt gtaccaacat tggtatttgt tatggataaa gatctggtag
    19021 aacctgcctg gccgattctt ccgtccattg ttacgttgtc agctccgtta aaaataacta
    19081 cagcattatt aacgtttccg gcgattgtaa atgcaccgtt tgtattgata gcacatgatg
    19141 taaatactcc gccgtttaat gaagcagaat caggctcagc aaaatttgct gtgattgtta
    19201 ctgtaactgc gccgttacca tgtgtaccgg cattgatagc attaaatgct gtttttaaat
    19261 tcgcataagt tgctgcactt ggggtaacat caacttgtga attagcgaaa ttgcccattg
    19321 ctaaaaatag aacagcaacg aacaaaagtg aaataatttt tttcatttcg tttttagttt
    19381 agtttgtaaa aaattgatgt taaaaatagt ttctttttct ttaaaaatct acttcttttt
    19441 ttttgtaaat tttttccccg attcctccaa cattttaatt catattaata attgattgtt
    19501 gatattatca attaatattg ataatttaaa gaaaattaaa cttttatgaa taaatttaaa
    19561 ctcttttctt acagattcct ttatctgtta tgtggaataa ttttaattta catgacaatt
    19621 acaggaagtg aatgtcagga tgcactgaat gctatagttc ctgaagtacc cggtgacatt
    19681 gtcggcaact ggaaacttat cgaacagaca ggatctttgc aggatatatg tccggatgaa
    19741 accgtaatct ttcagaccag tggacaagct cagcttacat gtccaaactc aaacaccatt
    19801 acaagaaatt ttgaagtagt gagcaacgta cttacttata ctcagacttc tatttcctat
    19861 aatatggaat actcaaataa taatcaggaa ctcttgctct ctggttcaaa tgtagataga
    19921 aatctgaaat atcagagagt tgtcacagct tctgatcctg ttattaatgc acctttgaat
    19981 aaatcgaata attttattaa ctcatctgac gaaagtagat aactcatgaa aaaattacta
    20041 ttctctccac taataattat tattgtttca ttttttatag gttcagaatc ctcagaagta
    20101 aataaaattt cctctgacgg tataaagcaa attacaccta cagtcaaaaa agtaaatgat
    20161 gttatccgcg aaaaaaaaat gtcatataat ttcacagatg taaatgtttt tgagtttaat
    20221 aaaaataccg gtcaaaacga caatacatct tcttttgtaa ataaggcgac aattcttaaa
    20281 ctcaacactt caaaattaaa agacctgatc cggctaaata aggagaatat tgaattcagg
    20341 atccctttaa ctgaaaatga aaatactgtt ctcgagttga cacgcactca gccggaatca
    20401 gatgatttta aacttattga aaaatcggga aatggtgata aggtcgttcc ttacacacag
    20461 ggtctacatt ataacggact tataaaaggg aatgaaaatt cactggcatc acttagcatc
    20521 tttgaaaata tggtgattgc attgatctct gatgaaaacg gtaactacgt cctcggcagt
    20581 attactaatg atgataagtc agtttcagat aattacattt tttataatga cgctgatatg
    20641 aatatcagaa ataatttcaa atgcggagtt gacggtattg aggaaaaatt tatcagacct
    20701 ataaaagtaa atgagaatac ggttaataac tatcagacgg atgatccaag ccgactgccg
    20761 gtaaaggttt attttgaagc tgataataaa atgtatcagg ataatggcaa caatactaca
    20821 aacgtaggaa atttcattac ggcaatgttt aacaatgtca aaacacttta tcagaatgag
    20881 cagattcctt ttgaagtttc ttcagttgca gtctggaccg gaaacgatcc ttacagcagt
    20941 ctgaatgatt cttatgacgt attgcttaga tttggagcaa atacgcagga taattttcag
    21001 ggaaacattg cacatttact ttcaacacga aatgccggac tcggcggaat tgcatggata
    21061 agagtaatgt gcgcaaatta caatgcacag gattctgccg gaagatttgc attcagtaat
    21121 attgacaaca attatcaaag ttacccgacc ttctcgtgga ccgtacaggt agttactcat
    21181 gaaatgggac acagcatggg atcaagacat acgcactcat gtacctggcc ggtgaacggc
    21241 ggttttaaca gagctattga ttcatgctac aatgctgaag gcaactgctt taatcagaca
    21301 agagcaagag ttggaacaat tatgagctac tgccatttgt ggactgcggc tcagggcggc
    21361 gggattagtt ttacttcagg attcggtccg cttccgggtg acacgatccg attgagatat
    21421 gcacaggctg gatgtctggg acaagttacc aattcatctg aacaaccttc tacttttgat
    21481 ctgtatcaga atgctccaaa cccatttaat ccggaaacaa aaattaaatt tgatctgccg
    21541 gaggaagcga ctgttacttt aaaagtttat gacatcagcg gtaaaacagt tgctgtactt
    21601 atctcgggag aattcagatt acccgggttt tatgatgtga acttccgcgc ttcagattat
    21661 aatctctcca gcggaattta tttttatgaa ctaaacacag gcaccttcag agaagtaaag
    21721 agaatggtcc taataaaata aacatataca atttttattt caaagcccgt ttaaatgcgg
    21781 gcttctttta taaatttaat tttaataaaa ttaaaaatgg tttttaaaat attaactaat
    21841 gtttttttag gtataatgct aatggtaatt atctctgcat caagctgttc gtcaagtgag
    21901 gagtcagacg atttcaattt cggaaaagga aaatatagat tcaaaatgtc cgacagcaca
    21961 ggtaaagcac ttgtcagcgg gattatagaa gtaaagacct acgtaaatga agacataaca
    22021 gggacattgc ttgtcacaaa aaattatgta gatgattttc caggattcac atctatgagc
    22081 aatgattttt caggtaatat aaacagagta gagaaaacag tttttattaa tactaatcct
    22141 aaaatagctg acagtaatgt attctggaat atggagcttg gatcaaattc cgtttcaggt
    22201 gaatggagat actcgatctt cagaggaaat acaacttttg gaaaaattaa aattacgaag
    22261 tactgatcta agtttttatt ttgtctttca gcattaagtt ttgaattcta caattagctg
    22321 tttttgattt ttgtaagtaa taatgatttt aagtaattta tttttattaa ataatctaat
    22381 aatcatttat gaaaaaaaaa ttaaccgcat tgactatcgt tttacctctt ctttttattt
    22441 ttttctacgc tgattattcc cattcatatc caagattcgg tgcatatacc ggcaacaagt
    22501 gcatggactg ccatgtaaat cccactggcg gacttatgag aaataaaggc ggaacgtatt
    22561 atgcaaagaa aaatctgaag atgaaaatgt ttaaagagat tgcagggaag acaaaatttt
    22621 ctccggaaat cactaaaaat attacactcg gcggcgatgt aagagttgct caggttgaca
    22681 atgaagtgga cggctctaat aatttcaatt catttcttgc aatgcaaggt gacatttatt
    22741 ttaatgctga gctgaataaa attctcagcg tctttgcaac cacaggcatt tatatccccg
    22801 gtgtccagac agagtatgaa gtgtacgggc tgatctcaaa tctgcctgca aatacatata
    22861 tgaagatcgg aagatttgct ccgaattatg gcatgaggat cgttgagcac agagcctatc
    22921 agagacagct tattctgaat accccttatg atgcaaacac aggttttgag cttggcgtgt
    22981 ctcccgactg gttcaatgcc agcgtgggtc tttacaatcc gatgaatgca agtttcctga
    23041 gccaggaccc gcataagatg tttacaggat ctatggattt tacattcggt gtagaaaact
    23101 gggattatac ttttaatttc ggcggatcat ttttaaataa tccttttaat acaagggaca
    23161 caacttctac ttccattatc acggcactga agcaggctta cggagctttt ggtaagatag
    23221 gtataatgga aagggtcgca ttacttggcg aacttgattt cgaagaaagt aaatctgaca
    23281 gacctttgcg gcgaagtctc tactggtttg gtgaacttga tttcataatt atacagggac
    23341 ttgagctaag aggtcagtat gaatattatg acagaaacag agatctggaa ggagatgatc
    23401 ttaaacggat cagcacaggt ctgggaatat ttccattcta tggatttgaa acggagctta
    23461 tggtaagatt tgtactcgaa gaacctgacc agtcaaataa tgaatggcag tggaattttc
    23521 atttttattt ttaaagatca gatattcatt tagataaaat ctcttttaca aagccggttc
    23581 tttatttgtg ttaattttta tttcagaaag aagtgtcttg tggacaggac atttttttga
    23641 aatctcaaga agtctgctga tctgttcttc actgagatca cctgtaaatt ttatactgac
    23701 atcaataata tcaaccttac catctttggt ttcacagtca cggcagtctt cagcatgaat
    23761 ctttttattt gaaagatcaa tttcaatatc attcagcgga taatcttttc tctgagcata
    23821 catccgcatt gtgaccgctt tacatgagcc tagagctgaa agcagcagtt cgtatggatc
    23881 tgcgccttca tttgtcccac cctgatcttc cggttcatcc gaaaaaaaca tgtgactttt
    23941 tgaatagatc tcggatttat atccttctga atttctgact tttatagttg ccatgatatt
    24001 gttttaattt ttaaaaataa acttactgat acttcagtaa tttaagttaa tacattttca
    24061 taaagatata aaattcaaat aaatttttat acttacaata tttatcaaac ttaagtattt
    24121 tattttaagc aaacgataac ggaggtaaat tataaaatca tcagatactg acccaaagta
    24181 caagaaagaa actaaagatg tctcacagga agaaaagatt tttcttgagg gtccaaaatc
    24241 ccgcaaaaga gaatttattt ttttaattga aatattttgg gaattcataa aaggtttcag
    24301 aatatttcat tttttaggtc cgtgtgtaac agtattcgga tctgccagat ttaaggaaga
    24361 ccatgaatat tataaaatgg cgcgtgacat tggagcccgg cttgttaata ttggatttac
    24421 tgtaatgaca ggaggcggac caggaattat ggaagctgct aaccgcggtg ctaaagatgc
    24481 cggaggaaaa tcagtaggat gtaacattga attacctttc gaacaggatc cgaatccata
    24541 catggataaa tggtttacat tcaaatattt ctttataagg aaagtccttc tgctgaaata
    24601 ttcatacgcc tttattgtaa tgccgggagg tgcaggtact atggatgaac tttttgaaac
    24661 cctcacactt attcagacaa aaaaaatatc agactttccc gttgtaatta tcggaaaagc
    24721 atactggaaa aatatgctcg aacttcttga tgactttgtg aaaaataaaa ccataagccc
    24781 ggaagatatg aaactcttta ttgtgacaga ctcagtagat gaggctattg agcatattga
    24841 aaaatttgca atagaaaaat acaagcttgt aaaaaagaat tattctcctg tgaagatctt
    24901 tggtgaaaaa aagagctttc agttccctga ataatattta cacccatccg gatctgtcaa
    24961 gacttctgta ctgtatcgcc tcacttatat gagccggtaa aatattttcc gaagcgtcga
    25021 gatctgcaat ggtcctgctt acttttaata tcctgtcata tgccctcgct gacaatccga
    25081 gttttgtaat tgccatcttc atcagatttt cacattcagt atcaattttg caatattccc
    25141 tgatctcctt tgaagtcata cccgcattag aatagatccc tttcttttct ttaaatcttt
    25201 cgagctgaat atttcttgct gctattacac ggcatcttac cgcttccgat ttttcaagat
    25261 tggtcttact cgaaagttct gtatatttta caggattgac gtgaatgtga agatcgatcc
    25321 ggtcgagaat cggacccgat atttttgata aaaatttctg aatatcataa tcattgtaca
    25381 tgtcagtttc tttagcagtg ccggcgggag tcgggttcat agcggctacg agcatgaagt
    25441 tgcacggata ctcaactgtg atctttgatc ttgaaattgt aaccattctg tcttccaaag
    25501 gctgacgcat cacttcgagt acattctttt tgaattctgt aagttcgtcc aggaagagca
    25561 caccgttatg tgcgaatgag atctcaccgg gcttagcatt cggaccgccg ccgacaagcg
    25621 aaacgtcact tgctgtatga tgcggtgaac gaaatggtct tgtagaaatt atcgcagtac
    25681 ctgaagttaa aagtcctgca atagaatgta tctttgttgt ttctaatgct tcctcaagtg
    25741 acagaggagg aagtatagta ggcagtcttt ttgctaccat agtctttccc gagcccggaa
    25801 gtccaatcat tataatatta tgcgcaccgg ctgctgcgac ttccatcgca cgtttgacct
    25861 ctgcctgtcc tttgacatca gcaaagtcca gcagataatt tttttccagc tcaaataatt
    25921 tttttacatc tattgtaaac ggcttcggcg tgatctcgtc attgagaaaa tctacaacct
    25981 ctgtcagagt atcaaagggc tgaacgtcaa taccttcgac tattgcagct tcctttgcat
    26041 tgtcagtcgg gagaattatc cctttgtaat tatttttttt tgcttcgagc gagattggta
    26101 aaactcccgt aaccggtctg agctttccgt caagtgaaag ctcacctata aatacataat
    26161 ctctcacttt cacaggagag acttgcgatg tctcacagat cagtccgact gcgatcggca
    26221 gatcaaatcc cgaaccttct tttcttacat ctgcaggtgc gagatttaca gtatatcttc
    26281 ttacaggaaa atggaacatg gaatttttta ttgctgctga gacacggtca cgggattcct
    26341 tgactgcatt gtcaggaaga ccgactatcg taaaattgaa aagtccgttc tcaatgtgtg
    26401 attctaccct tactatatat gcatcgaccc catatgtagt ggcagtataa acttctgata
    26461 tcatggcgat aaaaagttta atttatgaaa tgcgaaatgt agttttgttt tctgaaaccc
    26521 gaccgggttt tgactaatcg ttcttttttc taaatgctat atctattggg attcctttaa
    26581 atccaaaatg ttcccggagt tttttctcga gaaatctttt gtaattatca tttataccct
    26641 caggatcatt cacaaaaaat atgaatacag gcggagagcc tttgatctga gtgatgtaac
    26701 tgattttaat ttccttacct ctgagtgact gaggtggagt agctctgatg tcatttaaca
    26761 gagcttcgtt gagttcactt gtttttactt tctttgcacg ctctttatat attgaaacag
    26821 cttcttctaa tactttatga attctttgct ttgtcagagc agatataaaa acgaatttta
    26881 aataagtaaa acttttcaaa tgttctgtaa ttttttgttc aactatcttt gcagtgtttg
    26941 catccttttc tacaaggtcc catttgttta taacaatgag caggcctttc cttcttctga
    27001 ctacttcctc taatattttt acgtcctgtt tgtcaagctt gaatgtcgca agtttcatgt
    27061 cagaagactt gtcaagctcc tctattaaca aagtcgcgtc gatcacaaga atggctacgt
    27121 tgcatctttc gattgctttg taagctctta tagtagaata aaattcgata gactctttta
    27181 ccttggtttt ttttctcaga cctgcggtat cgataagtaa aacatcgtca ccgtagtatt
    27241 tcatattgga gtcaatagta tcacgcgttg tacccggaat gtcagtaacg atatttcttt
    27301 cttcatcgag aagagcattt gcaatggaag acttgcccgc attaggtttt ccgatgatgg
    27361 caaattttat tacgttgtct tctttttctt cgcttgcaag aggaaagtca gcagtaataa
    27421 tgtcaagcag atcgccggag ttgaatccgt ttatagcaga catagcaaaa ggttctccca
    27481 gtccaagtga ataaaattcg agagcatttg atgtcctctc cactatgtca attttatttg
    27541 ctacaagtat gatctttttt gtttcagtat gtttacgcag catatcagcc aatactttat
    27601 cgaccggatg cagaccattc tttgcatcta cgacaaagag aatttcatca gcttcatcta
    27661 ttgccagctg tacctgtgca cggatggctt tttcaaactg ctcttcggaa tgaggcacat
    27721 atccgccggt gtctatgaga aaaaatttct ttcccgtcca ttctacttca ccgtaatttc
    27781 tgtctctcgt cacgccgctt tgttcatgta cgatcgctgt tttctttcca acgagacgat
    27841 tgaataaagt tgattttcct acatttggtc ttccgactat tgctactaca ttttccatgg
    27901 aaacaattta cttttattaa aacggggatt caatgaatta aactttattc aattttaatt
    27961 ttaaataata attcattcaa aattacatat gcaatttaca ataaacaatc atatattcaa
    28021 agttgtaatt ttcggtgttt gtgaaaaaca cttatcacaa atataaacga acaatagaaa
    28081 ctgtaaatat ttaattttat actaaaactt ttttacgaat gtaaaaaaat cctgctgcct
    28141 ttttatatct gaatgtatta taaatgtcat ttcaattata tttacaaaaa ttaataaaat
    28201 ataaactatt atgtctaaaa aattactgtt cattttactg ctgtgtttta tgatttcgtc
    28261 ctgtggtaaa gataagtctg atacaaatac tgaaaattca aaagaaacaa aagctccaaa
    28321 agctgagaag gtggaatttg taatgaagga atacaaaaag acttacaaag actgcgatca
    28381 gaattcagaa ggatgttcat acattaactt caactaccct gagattctaa atgcggatat
    28441 aaaagacaaa gtaaactctg cgatcggcac atatcttcgg gacagtattt attcaatcga
    28501 aggtaaaaat tttaaaaatt tcgatgaact ggcatcaaca ttctttgctg aatacgacac
    28561 tatgaaaaga atttcatcag tacaggatct tgcttttgtg cttgatgaaa aggcagagat
    28621 aatttccgag acagaaaagg acttcactgt ttctatcagc tactacatat caacaggcgg
    28681 ggcgcatcca aattcattta tgagctattt tgtatttgat aaactcacag gcaacatcat
    28741 tacactcaat aattttttca aaccgggtta tgattcttta cttaataaac ttgttgacgg
    28801 agcttataga aaagaaaaag gtctctcgcc gactgatgat ctacagaaga aaggtgatct
    28861 gtttgaaaat aaaattacat tcaataataa ctttgcggtt acaaaagatt ctttaaaatt
    28921 cttttacaat aattacgaaa tagctgcata tgttttcgga ccgacggaaa taagtattcc
    28981 tcttaatgac ctaaaagagt atgtaaaaca ggaataaatt ttctgaaaaa gcattgtaaa
    29041 attaattgag ttcaaagctt ttcaggtctg aaaattcaat attagctctt ataaagaatt
    29101 gaaatcttat tctattaata atatattaat aaaaaatcga tatgaataaa aataatccga
    29161 tggaagaagt ggaaaaagag gtgattaaag agatcatcac tgaatcatat gaaattaacg
    29221 aagatgacgt aacgcgtatt gtgaataaag aaaaagagat aaataaaaaa acatcaaagc
    29281 ttgatcttgg gaaatatatg agatttatca aacagataaa attgttactc aatcttttaa
    29341 aagattacag aaacaaagct tatacagatg ttccgtggag atcgattgca cttttgtcag
    29401 cggctgtact ttattttgta aatccatttg atgtaatgcc ggatataatt ccgctgcttg
    29461 gatttgctga tgactcactt ctgtttgctg ccgtattcaa atccgtacag actgacctgg
    29521 aaaaatatgc agcatggaaa ggcgtaaaaa ccgaaaattt attttaacat aatccaaaaa
    29581 aaatcgaaag aggttatatt taattgtccg acaaaataaa agttcttttc atcggtgaca
    29641 ttattggtga tccgggttac aatcttacca aattaatgct tccaggctat gttaccaaat
    29701 acaaaactga ttttgtaatt gccaacggag agaacattac cgaaggtaaa ggcattctcg
    29761 aaaaggacgg taaaaaactt ctcgagctcg acatcaatgt acttaccggc ggaaatcata
    29821 ctatggataa aattcaagct cataaatatc tcatggaaga gaaaagagtt ctgagaccta
    29881 tgaattatcc gaaaggtgct tacggacacg gctttggaat ttataatatt ccgggtactg
    29941 atctgaagat cggaattata aatctacagg gtcgcgtatt tatgaaatcg atcgactgcc
    30001 cgttccgaac atttgactgg gtctatgaaa aaataaaata tgaaacgaat attgtcttcg
    30061 ttgattttca tgcggaagct acagcagaga agatcgcttt cggatggtat gcaaacggaa
    30121 gagcttcagt ggtagtcgga acacatacac atattcctac tgctgacagc cgggtactaa
    30181 gtgatggtac tgcttacatt accgatgtgg gaatgacggg tgcttatgat tcggtgatcg
    30241 gaatgaaaaa agaaggatcg ataaaaagat ttatttatca aactcctcag aaatatgaag
    30301 taggagaaga agacgtaaga ttctccggcg tgctttgtga aattgacagt acatcaggaa
    30361 aagcaacttc aataaaaaga ttattcagtc ctgaatttta acccagtttc ctcaatatga
    30421 tcagagtccg aaaaacacgt gactctgagg gaatataatt tttaaatttt tacaaaataa
    30481 tttaatcaca aaatattatg aaagaaatga aatcatcacc tcttaataag gaggtttaca
    30541 attatatact cgacagattt gtacctgagg atgaaataac agaagcgatt atccgggaga
    30601 ccgaagaact tgaaattccg ctaatccaga tatctcctga tcagggtagg tttttataca
    30661 tgcttgcaaa aatgctcaac gcaaagaatg ctcttgagat cggtactctg acgggattca
    30721 gtggatatca tattgccaaa gctcttccag aaggcggaaa gcttatcaca ctggagcttg
    30781 aaaagaaaca cggtgaaatt gccgaaaaat attttgagaa ggccggtctt aaagataaaa
    30841 ctgaagtgag aatttcacct gctatagatc agcttaaaat acttgaaact gaaaataaaa
    30901 aattcgatct gatcttcatt gatgccgaca aagttaatta cataaattat tttgaagcat
    30961 cactcaaatt gtctcacagc ggtacagtaa tggtctttga caatatgaca aaagataatc
    31021 gtgtagttct tgatgccggt gatgatgctg atctgaaagc aatacagcag accaatgacc
    31081 tgctgtcact agacaaaaga gtagaatcat ttttgctggc tataggtgat ggatttttga
    31141 tcacaagggt taagtaaaaa attacaatta gataaatctt ttcatatctg cagggaattc
    31201 ctttcctgat aattaaacta cattaaaatc gtaaatacct tttgttttat actctgattc
    31261 acttttttga aacggagctt caaaaggaat ggcattccca aacgcatttt ttcaacgagc
    31321 actatctaaa acttttccgt ttcccaaaaa ctttttactt tcctgaaaca gagtttcaaa
    31381 tgcaacggca ttcccgaacg gagtttggga acgagcacga actcagagtc tttgagcctt
    31441 cggggtaacc acaatatacc cggacagttt gcacatcaaa cattgaacat tgcacattga
    31501 acattgatta gttatttttg aacggaggaa aaaaaatatg aaagacataa aaaatgtttt
    31561 aaagaacgca gtatcattta ccaaaaatga aatacaggta attattttca tagccgtagt
    31621 aacggcggcg ggatactcgg tgaaatatta taagtcaatg acagaagata aaaataccgc
    31681 ttatgattat tcccgttcgg acagtatttt taaagagaga tctctgaaga tctatgccgg
    31741 taataaaaaa ggaataagtg ataaaaattc tgcactggat gaaccaggca tgtacaaata
    31801 ttttgagatt gcagaagatt ctgtaaaacg cagtgaaaag atcaaagctg ataatgaaat
    31861 ccctgaattt attattgata taaacactgc cggaattgcg gaactgacag aacttccggg
    31921 aattggtgaa tctattgcag gtagaattgt agaatacaga gacaagaata agaggtttgg
    31981 aaatatcaat gaaataatga aagtcaaagg tattggcaag aaaaaatttg aaaagttaaa
    32041 aaaatatatc aaagcggaat aatataattt gaaaaagatc aacatcggtt tcagtggtaa
    32101 taatatgaga ataatggaac tcaccgatac aggtgaagtg aacagactgg aagaagttac
    32161 tttgagtttt aatcttgata atgacaaact tctgaagaag cacagaaaag aataccttga
    32221 agaattttcg gaaattataa acggtatact tgatacttcc gggctcgaac catatgaggc
    32281 aaatatttta attgatacgg gacttgcttt catgaatatc atacctgttg atttcagcga
    32341 ccgaaactca agtattaatg cgcatattca ctgggatcta tcaaattatt ttcctgaaac
    32401 atataaagat tataatgtaa aatgtttccg cttaaacaat tatcctgtca gtgaaaaagt
    32461 cgatgaagta ttgcttattg cagtaagtaa tattaaaact gattttctga aaaatgtatg
    32521 caacaactgt aacatcattg tcaaaaaaat cgagatagat cagtttgcaa ctgaaaaata
    32581 cattagaaat aaaaaatctg ttgctgaagc gtcgctcata ataggtttta gaaacaacag
    32641 actcgatctc agtcttattg taaatggtgt tctaaaatat tatgattatg aaattaccga
    32701 aagagagatt tttaaactgc ctcttctaag acaattaaat tttttcttaa aaagattcgg
    32761 taatattaaa attgttgaga tcaatattta cggtgaagaa aaagttacag atctgaaaaa
    32821 tttcctgata gagagtgtaa agaatatacc tgatcttaat gttggaaata ttgatatact
    32881 ttatgataca gagatacacg aaagtaaata tgcacctctt tacggtcttg cagtcagcag
    32941 caatacaaca gcatgagaat tatttcagga aagtataaag ggatgaagct gagttctttt
    33001 aatgaaaaaa atatcagacc tacatcagac agggcaaaag aaactctgtt caatattctg
    33061 cataatttaa tggactttga aagcatgaaa tgtctcgatc ttttttgcgg ttcaggtgcg
    33121 cttggacttg aaatgatttc acgaggagca gacagctgtg tatttgttga tatgaatatt
    33181 gatgttgcaa aaaaaaatgc tgcatattta tatgttgata ataaatgtga atttgaaaga
    33241 tctgatgtcc ttaattattt aatgaaggtg aaaaatgagt ttgatgtagt attttgtgac
    33301 ccaccttatg attatgaaag ttatgatgat attataa
//
LOCUS       DAORVQ010000012        33128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_413_length_33128_cov_6.152934, whole genome shotgun sequence.
ACCESSION   DAORVQ010000012 DAORVQ010000000
VERSION     DAORVQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 33128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33128
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_33128_cov_6.152934"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..2254
                     /locus_tag="PLX80_02140"
     CDS             278..2254
                     /locus_tag="PLX80_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19626.1"
                     /translation="MKKAKISITNPKTSKRNEAVKSYEGFDKQTNLQGFLKYTVYFIL
                     TALPVYLAYNYYANALLINGYNSFQLDDGWIHLTFAKNLSEYFSFSYYKNEMVTAGST
                     SPLYTFIIAAGFFITDNEMILSYVIGVLFFAMAAFVIFRLCMHDFNKDLLASLVCAGL
                     FILDRNMNFISLSGMETTMYVFILLLGAYLYKTKKTYSLAVILGLVIWSRPDGVAFIA
                     AVAVDIAIALFYFKNEEIKKVYNKEKLLKMSLIFFVIAGAYFLMNYVIAGTFFPNTYS
                     AKIAYFSDIDKKFSYLTEKVYPFFSNLSYSFFVAGFLFAVLISVYNLVKKRTSENLLY
                     ILFILFFLSLYMIKLPEINRFGRYIMPLIPFFILLSISGYREILIIINKFLKNAFITR
                     LAFIIFAGVLFYQSFNAYETFKMFYAEQCKYIYDRQVKTAMWLKENTDENDVLGVHDI
                     GAIGFYTGRKIIDVAGLITPYLNERLTEKDYSKIMKDYLQQQGVSYIAFLREWYRSIN
                     QVPLFKTPDYSPQEVMEVFEFIPDSTYIISKNANYMMDKALMSYKEKDGNEIIKDMNN
                     ILELEPTYAEAYYLRAYGYSLLNNDEKYLEDLKNSVRYFPGLIIAQKNLGMLLMEKGK
                     YSEAESHLRKALELDPANKSVQESLRVIEEKIAK"
     gene            complement(2341..3222)
                     /locus_tag="PLX80_02145"
     CDS             complement(2341..3222)
                     /locus_tag="PLX80_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19627.1"
                     /translation="MKKVLFIITLFCSTQTIASDTSLVRYFPLKTGNTWYYKTTFSPI
                     PFPPTFHKLYIQKDTVINGHIYFLMNRYGMNVYVRIDSLSGNFLLYSPGSGCGSYPDD
                     KIIDSLASNPGDEINCLFQAIFTRRCMSITNQNVFGILTEVKEFFHGGLVIQDIEYAK
                     GFGEIYSCSGDPYCSGFTYLLGCKIDGVVYGDTILSDVKLISNFVPDKFSLSQNYPNP
                     FNPKTVINYDLEVAGNAKLKVFDILGNEVAELVNEKQSAGSYSVEFDGSGFASGIYFY
                     RLDAGDFVETKRMILLK"
     gene            3454..3527
                     /locus_tag="PLX80_02150"
     tRNA            3454..3527
                     /locus_tag="PLX80_02150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3488..3490,aa:Ala,seq:ggc)
     gene            3565..3873
                     /gene="rplU"
                     /locus_tag="PLX80_02155"
     CDS             3565..3873
                     /gene="rplU"
                     /locus_tag="PLX80_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQY19628.1"
                     /translation="MFAIVNIKGHQYRVQENQKVFIPKLKDEVGSKVTFSDVLMFSKD
                     DKSFEIGAPSLEMKVEATVLDHVKDDKVIVFKKKRRKGYKVRRGHRQNYTQITIDKIS
                     "
     gene            3976..4236
                     /gene="rpmA"
                     /locus_tag="PLX80_02160"
     CDS             3976..4236
                     /gene="rpmA"
                     /locus_tag="PLX80_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQY19629.1"
                     /translation="MAHKKGLGSSKNGRDSNSKRLGVKKFGGEAVIAGNILVRQRGTK
                     FLPGQNVGLGKDYTIFSLIDGKVNFTTKKGRKTLSVLPYESA"
     gene            4323..5258
                     /gene="mdh"
                     /locus_tag="PLX80_02165"
     CDS             4323..5258
                     /gene="mdh"
                     /locus_tag="PLX80_02165"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQY19630.1"
                     /translation="MKITVVGAGNVGATCANVIAQKELANEVILLDIKEGIAEGKSLD
                     IWQTSGINNYDTRVIGVTNDYEKTAGSEVVVITSGLPRKPGMSRDDLISTNAAIVKSV
                     TENVVKHSPDAIIIVVSNPLDVMTYCAFLSAKLPSSKVMGMAGILDTARYRAFLAEAL
                     DCSPKDISAMLLGGHGDTMVPLPRYTTVSGIPVTELIDDEKLNAIVDRTKKGGGELVA
                     LMGTSAWYAPGAAAAQMVESIVRDQKRIFPVCAWLQGEYGMNDIYLGVPVVLGKNGIE
                     KIIELKLNEDEKTLLTDSAKAVKEVMGVLDELNKK"
     gene            complement(5296..5715)
                     /locus_tag="PLX80_02170"
     CDS             complement(5296..5715)
                     /locus_tag="PLX80_02170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY19631.1"
                     /translation="MHKEILTLKSERNEIVKFEDLLDSVNEKFGLEMEKFINLKIACS
                     EALINAIVHGNNEDPLKKVYTEIIYGDETIIIRILDEGTGFVINSVPDPTTDENILKE
                     SGRGLFIIKSLVDDFICTSTGSGTEFIITCNKKNPVT"
     gene            complement(5763..7637)
                     /locus_tag="PLX80_02175"
     CDS             complement(5763..7637)
                     /locus_tag="PLX80_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19632.1"
                     /translation="MLIPVSAMSQSGSILTDTVYSTNIFETNFYNDLNSANFNSLLNY
                     ENNIERFGFLITNNFLSNVSKLNKNFFRDFNSLNLLMSYDVKEDIDVGVGFQNKFYTD
                     DKTVETNQNNSNYFYANMDASMMNSVFFNTKLGYKTEDQIGEFNSGFSGFFETQTNNY
                     NLNDYITNGNLILFYEDLDQKQNHNYEINTEIYKRFAQQVDNTGRLTFYNQKNDFFFP
                     ASPGVALLYDVLNNIESRDENYFYIGDMLNYGFTDDIILSLGGNYMNRIIKREYKYKP
                     TSNNVFFENTYDNQITENNLEVYTNLNYNWRNLFTQLRVLISERSENHTLINPEGLTA
                     AQISELERAEKNKNNNSRRTSLLFNAYYPLSNTNSFNFTGSTSLLLYDTDFQDNFDDR
                     DESESIVSASYNFNNLNNFYVETRFEYIVSKLSYIYSQRSANNYTNRVYKLGSYSSFK
                     PVERFTTNNFFQVLANYTVYEFEDLISQVQSFSYRQLSIRDSSSYNLGRGFKLNFAAE
                     LKFYEQGEFLNNSFSVKPITFYAEQFYMPGINYMINNFLNFGVGYKYYIQDRYNYDEA
                     EKVLANTYKSYGPVGIAEMYMNNNSLIKFILGLDQIEYLNPSQTNSSVNFQLNILWNM
                     "
     gene            complement(7666..8427)
                     /locus_tag="PLX80_02180"
     CDS             complement(7666..8427)
                     /locus_tag="PLX80_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19633.1"
                     /translation="MILVKDLTKKFDDFTAVNDVSFEIQKGEICGYIGTNGAGKSTTV
                     KILTGILDFNKGSVLINDLDIKKDPVEVKKIIGYVPENANLFNSLSPVEFLNFTGMVR
                     DLTDKVISERINSFAEMFGFTDLVNDSIGNLSKGNKQKVMITSALIHDPHVIFLDEPL
                     NGLDTNSIFVFHDLIRFLAGKRKTIFYCSHHLNIIEKISTKIILINKGRIELDINTSE
                     LSSLENYTDLEKLFRDLNSEDSSESKKFNFEDLYN"
     gene            complement(8446..9180)
                     /locus_tag="PLX80_02185"
     CDS             complement(8446..9180)
                     /locus_tag="PLX80_02185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY19634.1"
                     /translation="MNLKAEIIEEDFDYQIDGLTYQGFIAYDNSIKGTMPGILIVHQW
                     KGLGDYEKMRARMLAELGYLAFAVDVYGKDNRPQTREEAGKEAGKYYADRDLFKLRVN
                     AGLNEMMKIDNVNKNNIAAIGYCFGGGGVLELARSGADIKGVVSFHGSLKSGNPDEAK
                     NIKAKVMIMHGAIDPNVPEEDVIAFKKEMEDGGVDYVLAEFSGAVHSFTDKNAGNDPS
                     KGSAYNEKADIRSWDYMKVFFAEIFN"
     gene            9413..10141
                     /locus_tag="PLX80_02190"
     CDS             9413..10141
                     /locus_tag="PLX80_02190"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY19635.1"
                     /translation="MQDYQENIVSSGQSESDLEKPKILSSFFFSGVFVLLLWIIQFLQ
                     WSLAKEFATFGVLPRTLTGLKGILTAPLIHADFSHLISNSFSLFLLLFGILYFYRSSA
                     VKVFIIIYLVDGIMVWLFARMSYHIGASGLVYGFAAFLFFSGLFRKDRSSMALSLLVV
                     FLYGGMVWGVLPVKPEISFESHLFGALTGIVCAFIFRKNDPPEKYEWEEEDYEEDDDY
                     DDGKEPEDVEIDEDAEEDDFRRLS"
     gene            complement(10328..12790)
                     /locus_tag="PLX80_02195"
     CDS             complement(10328..12790)
                     /locus_tag="PLX80_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY19636.1"
                     /translation="MRILIFIFLILSFTSAYSQTEPDNNQRKGNNLKPEFDDGSVSGY
                     VFDKLSNTPMESATVQLFRKRDSTLAGGAETDAKGFFIINDVAQGKYRLHVSLAGYNK
                     NMRDADVLNASAKDIVTDTIFLNSGTETDEIVVEGEKPYMEMQGEKKIFNVEESMSVK
                     GGSAVDILKNLPSVTVDIDNNVSLRGGSNIKYFINGRPVTGSITRILEQLPADQISSV
                     EVITNPSAKYDAEGSTGVINLVLKEYDDSGINGQLELSTGTQDNYNTGFNLNYKNKDY
                     KVSGYYDGRIATRFFNSAINRQNFFSTEEAQTDQYSNGRFRSNGNNARVELEFYPTKA
                     DVLTFNARYEERERNRGDTDNLTTYDNQNNLFQDYKALNNETETSNGYSLGLNYFKPI
                     SKKGHNLTGELSFATDKEYENENKQIDFLFPANLQSIFSIVNGSEINKEFNGQIDYVN
                     KFSENMGFESGIKYNVRNTNTDNYYYTQDTVSKVYLIDTALTDDFSFEDGVGAAYLIY
                     TGQAGDLNYNLGIRGENWNYNLDQILSSSNTSRNIFDIFPSAGISYKLSLSDEIGANY
                     TRKIRRPGYRELSPVTRVFSPVLLSKGNPDLNPEYINSIEFNYAKFFNEFSVIPSLFY
                     KNISDVITRTSVLIDSNVILNVPINANTERSYGAELLLNGMFANSITVNGSVSYFYQD
                     INSDTLGNNSNTTFSGRLFTNFALPFESGLQLTYFYSGKAITPQGTIDPVSSFDVAVS
                     KDFLNDKLNLNLRVSDVFNTTKFSGSSTTSLYTQEFTRARDSRMATLSLTYKFGTDSK
                     SKERKKKRQGNNDGNDSGDMDF"
     gene            complement(12994..14409)
                     /locus_tag="PLX80_02200"
     CDS             complement(12994..14409)
                     /locus_tag="PLX80_02200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY19637.1"
                     /translation="MKIKFCGADRTVTGSQHLLEINGKKILLDCGLFQGKRQDAYDIN
                     RTFLFDPEELHCVVLSHAHIDHSGNLPTLHSKGFKGSIYCTSATRDLCSIMLLDSAHI
                     QEKDTEYVNKRRAKKNQSPFKPLYKIDDARNVLKSFKTVSYGKTFCIDGLCEAVEVEF
                     FDAGHILGSAMMVLTIKENGRKFKFAFSGDIGRPGLPILKDPEFIGDVDFIISESTYG
                     GRIHDKANMMDQQLLAVLKEAVSRGGKIIIPAFSVGRTQEIIYSMSVLFNEGLIEKFP
                     IFIDSPLSTKATDIFKMHPECYDKEFSDMIIRGKEVFELPQIRYVQDVEESKSLNSVT
                     GPCMIISASGMAESGRILHHLKNNIEDPKSTILIVGYMAEHTLGRRLIESREEENASI
                     RIFGDEYFVHSKIVVLNSFSAHADRDELLEYFDKFDRNKLQKIFLVHGEIDQQEAFKD
                     GLKFQNFKDIEIPVKGQVFEI"
     gene            complement(14437..15057)
                     /locus_tag="PLX80_02205"
     CDS             complement(14437..15057)
                     /locus_tag="PLX80_02205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HQY19638.1"
                     /translation="MNLYDVKSLSKEFYLKDAVIVAKDLLGKLIIRKFGNKFAAVKIV
                     ETEAYFGDHDPACHAFGRVTKRNSVIYGPEGKAYVYFIYGSYYCFNAVCGKEGTGNAI
                     LIRAGEPVEGIEIMKKFRPNVKNDIELTNGPSKLCMAMNVDLSLNRTDITNTDSEIFI
                     AENDRIKRSEIITSRRIGLNKGVEFPYRFFIKENPFVTKHKFNSEQ"
     gene            complement(15497..17929)
                     /locus_tag="PLX80_02210"
     CDS             complement(15497..17929)
                     /locus_tag="PLX80_02210"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY19639.1"
                     /translation="MKKIAGKDFKTLKILSILFILVLSTKIISQPVWTIQNSGTSNDL
                     NKIYLKGYNMSHSLFVIGDNGTILRSINSGTSWQKINSNTTADLYTIGFRDNFGDTGY
                     CAGEGGVIIKTTDGGFNWFTIPSATASTIKDLKFTNSNAVPMSAVAVGENGTLLKLIN
                     GIWEAVQIDTADLNSIAIENNTRFTIVGNSGLILRSTNSGTNWVRINSNISNDLNQIS
                     YSSIVIGDSGVAFQIQNNNINIINTNTVNNLYDRNLNYDIVCGASGTILSFFQPIPTN
                     TNTNLRSIVKSDIDNCFAVGDNGLIIYTKSFYVSSGTNELNSNNISAWFKNNGIFNHH
                     PLGDGGFQWPKGENKYARYSSGHYLGALVNNEIRVSAVSYSSEYLPGYTAQSGTPHGN
                     GTPEYKIYKLIHNVNDTDRIKWPNSALGNSDQGAPVYFDSSMMTWKPVDYGNQTMFYS
                     YTDSYPQSHNYYNTSTAPLKADIKQINFSFNQPEKLKNVIYQEYRIINRSNEVWNEAY
                     FNFYSDDDLGESQNDAEGIDTNLRLAYTYNFTQTDYIYGNGPPAVGNLIVRAPLVYTG
                     NSNDTVFYYEGKNKNLKIGYKEMSLNSTVIYHDDSFEPSNYTQTYNAIRGFKNDGNPH
                     INPVTNQPTKFMYSGDPVTGNGWIKSNSGDMRFYQGFGPMTINPGDTQIIVMAQVIAK
                     GSSNLNSITKLREAAITAKEYYDNYFTDVVIGINNSVTSLPDNFVLNQNYPNPFNPVT
                     NINYKIPNADIVNLKIFDISGKEIATLVNQYQQPGSYSVVFNGNNFSSGVYFYKLTAG
                     KFSGTKRMMLIK"
     gene            complement(18456..19964)
                     /locus_tag="PLX80_02215"
     CDS             complement(18456..19964)
                     /locus_tag="PLX80_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19640.1"
                     /translation="MKPKLLFTLALLFLSFSSVSFAGPKISFTELITNAERDAQSVIS
                     AKQLAMQYHLPVTILTKNKTVINAIGIEDGKVVYSVITNSADIYSQSYVMFFVEIEKN
                     FDLSTSLIIYSKNNIVDNSGDTYKIILNPEVSGNQPILLIPDWTFDRVSAFNSVTGDL
                     IDTAFIHSNNPNLQSPKMALQAPNNFVYVSDQISDLVQKYDTNGLYVGFFAPASGVNT
                     SIVDNMRGIAFRPNYNLLATVGSGANQNTIQEFDLGGNPIGSFIPTGTLNSPFDIHYR
                     SNDILITNSSGGNDVISYDYNGTFISNFINSPNITFAQQLIRNDNGNLAVCGFSTPSG
                     LILFDSSGNYMRSLTGATGLRGAYLLGNGHYLVTNGSGVHEIDSASGSLIRTVLTGAN
                     FQYINKYLPDGLVSVNNISNEIPEKYSLSQNYPNPFNPKTVISYELQVTGNAKLTVYD
                     VLGNEVAVLVNKKQNAGTYSVDFDGSNFSSGVYFYKLNAGEFSETKRMMLIK"
     gene            20341..21021
                     /gene="aqpZ"
                     /locus_tag="PLX80_02220"
     CDS             20341..21021
                     /gene="aqpZ"
                     /locus_tag="PLX80_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HQY19641.1"
                     /translation="MKKNLSEFIGTFWLVLGGCGSAMLAAGFPDMGIGFAGVSLAFGL
                     TVLTIIYALAHVSGAHLNPAVSIGLWVGGRFEAKEILPYVISQILGGLAGAGVLYVIV
                     TGNGSQIGTFAANGYGEHSPGGYNLMSAFITETVMTFMFLIVILGSIDKKASPGFAGV
                     SIGLCLTLIHLISIPVTNTSVNPARSISQALFVGGWAIEQLWLFILAPIIGAVLAGLV
                     YKIFKTSE"
     gene            complement(22054..23607)
                     /locus_tag="PLX80_02225"
     CDS             complement(22054..23607)
                     /locus_tag="PLX80_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19642.1"
                     /translation="MKPSDDIFLLIKSMSGKEKSFFKKKFLLLIEESDNNYLKLFNEI
                     AKQIESGIDYDESRIKNGSYSGKFIKNLPFHKNFLYNSILNSLCYFHKDDNDKFSLRN
                     LLIKSEILSDKFLIDQSLKLLQKAKKTAVEKEIYNSQYEILNNERLIKKYKCSVEEYT
                     ILSKEIFENQYNLLELNRNLLDYYTLNENVGIFLRSEGSGRVRNKEKQIEFKKIFEST
                     LLSDIKNAKSFLAKYIFYNLWLQYYLTMEKFEDAYKCAKSAVELCENNMVKLKSKLDN
                     YIFSLNNLANCEIRNRKYDEAEKTVVKMENVLIIHSGSVTEVNKVFIFYSSSILRISI
                     YMESFNESKLVKAESDIQKNIILYEDKITIYQRIILYYFLSASNFIREEFENCIYWNS
                     KIFNLGRTDLSEDYQCYAKIIQLISYYELGYIDSMEYALKSAYHFISMKKKVYIYENI
                     IQKYLRKSFRVKTDKEMIEMFKEMKDELNVLLKDPMEKHAFDAFNIIFWLDSKIKKIP
                     MIEIIKESK"
     gene            23939..26362
                     /locus_tag="PLX80_02230"
     CDS             23939..26362
                     /locus_tag="PLX80_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY19643.1"
                     /translation="MKKTILSLQIIFVITIFCGSSANAQMYWNQAGDFPGTVGSHIAV
                     PNSSSLDLTSGFTLEALINPAAASFIAKGIISKSTGLNIRYAMRLVSGRIFILTNSAN
                     RLVSRASNPIPLNKWTHVAATLSSTGVYQIYINGILDTSNSLPGTLPTVTSDSLLIGS
                     TGPSTEFFGKIDEVRIWNTNLSPLEIFFLRKTTLGVSGTFYSKLVLSIPFQNNTGAGT
                     IFSAMDHSDKINHGFIRNVTAFDLKDRPSGIHHLSDCVNLGPSGFLTASDNPAVSPVT
                     KLTIECWIFPSTQNYAIVYKGPAFSSNPDYGLKVISGKLVGYINNTQINSEDSVKTER
                     WSHVAFTYFGATGRFEFFVNGKQGTTGNIAPANINNGSDSLFVGAFPLTSTLNGYIDE
                     LRITSELKTLHDINKQMFSSVNESNDNDAVTNVAYNLDGSTLPSTDDGPRLNLRGSSI
                     FIFNTSQFSSAIPHSPLINNSSGQFQSGYYLNMPQKRIPASGTAGTIRDTIEILSSET
                     ISDLNIYVAINHMREENLRITITSPLGASTDLFANTSLMDSNKNLVTIFDSDNDSSLI
                     SNRYVSFGPGIKPLFDIDAIFSGSNSKGKWILTIIDEGAADTGILISWGLQINEHNNL
                     PYNLECSSLIEGFYNSSSNSMTADTVRYFLRSITSPYSIIDSSKAKVNSSGSVNASFT
                     KAQPETNYFLAVKHRNSIETWTSTVIKFNQFTKQSSYNFTDLATKAFGNNMKQVDSSP
                     VRFAVYSGDVNQDGTVDLSDGSLIDNDAFNFAGGYLPTDVNGDEVSDLADAVFADNNG
                     FNFVGKITP"
     assembly_gap    26725..26824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27214..33087)
                     /locus_tag="PLX80_02235"
     CDS             complement(27214..33087)
                     /locus_tag="PLX80_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HQY19644.1"
                     /translation="MPGNVDVPTGAIVQNNTVSGYQQISSSDGFGIVVEGLNHSVLGN
                     TISLCDVGIQRQAGHLPYPGDGDQNNVADTYFGRGNSPQTCGIVMSGNILSNTVDTRD
                     VGANTGSGVVMNLNSTEMFCSIQTAIDDAQTLNGHVLTADSGYYNENVVINKEVTIKG
                     SGTTKESRPVVEGSASQTISVTVPNVTIDNVIVKFNQLAVNTGIRAAASGTFNNLTIK
                     NSCVFGTATTGAAIFNSFGIQLGTFGGVQYDQVNLDSNDIKHTGNSPLGRGVKTFNCY
                     GDWKNSIVRAFYAIQSGDIQGGILNITNDSLFGQTEINSLGNGTHSFANNVSNVSNAF
                     GSGTDFAMLELKNISNGSANIEVTGNTFENYVNFGVFSGRSSNVNIDNNTFTPDPTAE
                     LFKSIRIDSKQRTTAPQTAFTSNASITNNTFNGNAAFNQKGLSVELASSDSLSSIGTV
                     IVGTLGNENDFNTDVWRNISLNNQTTATSGDPIWTGTYVSTKEKVNVNVDGRNNNFNV
                     GAGLQSPNVMTLPNLFELEDRIQHKIDDGNIGFVLVKNNNDYVTVNSFVAPGITTPSV
                     QRGINAASPGFVVNVGPGSFTEQLEVNKDITLDGQGTGVTNIISPNTLTLSYVTSGVN
                     KPVIYVHDAAGVIIKDVTIDGAGKGNANNRFQGIGYRNAGGTVRDCEIKAIRNTPIDG
                     AQAGVGIFALADDGNARTLDIISNNIFDFQKNATSLSGIDLTANVDSNTITGAGPVSF
                     IAQNGVQIGFDAAGSVTNNIISNISYVPSTTVSCGVLLYYLTNPTGTVTTANNDLTGC
                     QVGIYYINVGGMINENTINSTLANNGTATYYGIVADPGDDPRVVPSPFEEDLLAGKNS
                     NLPDNPNIITTSIYRNTLISDGTNGTGIEMDALGTETLNATATENKVNGWDAGVVFYK
                     DAGATLNGVVNDNDLSSNTYALYDLTGVTQNASCNWFGSSDQFVISTKVFGAVSYSPY
                     LVNGTDNDAPTAGFQPVPGSCTGFPIIAYFVNDNSLAGDVFTTAVGSDANPGSTSAPF
                     LTVSYAVSVANDNDTIYIDAGTYQEQVIINKSLTITGADSAGASAAVIKAPLSLNLVP
                     NANAADHTPVVYVSDSGTVVDISHIGIDGDGRGGSKFYGIYYFEAGGSFTNSRITGVR
                     DAVYSGVQSGNAFFANHTYDVNFDHTIIVSDNVIEDYQKTGILINELNTHAIVTNNIV
                     TGQSIQNVNGQNGIQFGYGSYGTITGNTISGNQYNGPAADGAGGILLAGAGVDNFNVP
                     TGNVTIIGGPGALANNLFDNEVALFTDGGGFGYNSNAGITYNANTFATNYIHASLSAP
                     NLVPSGLNVYDKWIDNTAYTNIKYGQIQRSVNDASANHLLQVSAHTFTEQVEIHKAVT
                     LEGQGIGSTTILSPNILPLSFSTGPNNKPVIYVHDTSDVVIKNLTVDGAGKGNANNRF
                     IGIAYRNAGGTVTGCEIKSIRNTPINGAQAGVGIYAFSDNGNPRQLNVVSNNIYDFQK
                     NATVLTGADLSVRVDSNTITGAGPVAFIAQNGVQISSGANGSVRDNIISGLSYTPGTA
                     VSCGVLLFQPSGIDTTSRNTLTGCQVGIYYIDVGGLISENIINSTAVNNGTNVYYGII
                     ADPGGIPHVTAEPLDVVSTSSNRSGNILDNLVLNTEIYRNVLVSDSTNGVGIEMDALG
                     TETLNANVTENKVNGWATGVIFYKDAGAVLNATVNDNDLNTNTIALDNQSGVIQNSTC
                     NWYGATDNVTISAQITGAVNYVPYLTSGTDDQGGVIGFQPVPGSCNGIPNSTINIKVL
                     PEAFYNGLQQLNLSDTMTVYLRSDNSPYNILDTSVAVVDSLTFTGAFTFDLPSGNYYI
                     VIEHRNTIETWSKSGGEVFISGTTMNYDFTDLASKAFGNNMQQVDNSPLTFGIYSGDV
                     TQDGIIDLSDIAVIDNDIFNFITGYVPSDLNADGLVDLTDMTYADNNAFNTIQKVTP"
BASE COUNT        10571 a         5880 c         5415 g        11162 t
ORIGIN      
        1 tttgcgtctt tgtgtctttg tggtattaat taaaattatg ttttgagaca ctttcaaaaa
       61 aggaaaaaac tttaaaaaag aatgcttttg tctttgtgtc tttgtggtat taattagaat
      121 acttcttaag cataagtatt taatgaacct ccgtttcgta aaagttcttt taatagaaat
      181 ggcctataca gtatctaaaa ctttttcatt caatttaatt tttaatagaa cccgatataa
      241 aattgaaatt acatatgcat ttaattattt tgaacgcatg aaaaaagcaa agatctcaat
      301 tactaatccc aaaacttcca aaagaaatga agctgttaaa tcttatgaag gattcgacaa
      361 acaaaccaac ctgcaaggat ttttaaaata tactgtttat tttattctga cagcacttcc
      421 tgtttatctt gcatacaact attacgcgaa tgcactttta ataaatggat acaacagttt
      481 tcagcttgat gacggctgga ttcatctgac ctttgcaaaa aatctctctg agtattttag
      541 tttttcatat tacaaaaatg aaatggtcac cgccggttcg acttctccat tatatacatt
      601 tattattgct gccgggtttt ttattacgga caatgaaatg atcctgagtt atgtaattgg
      661 tgttttgttt tttgccatgg cagcttttgt gatattcaga ttatgtatgc atgactttaa
      721 taaggaccta cttgcttcac tggtctgcgc cggattattc atattagaca ggaacatgaa
      781 tttcatttct ctttcaggta tggagacgac tatgtatgtt ttcattttgc tgctcggtgc
      841 ttatttatat aaaacaaaaa aaacatattc tctcgctgtc attttaggac ttgtgatctg
      901 gtcacgtccg gacggggttg ctttcattgc agctgtagct gtcgatatag ctattgcatt
      961 gttctatttt aaaaatgaag aaatcaaaaa agtttacaat aaagaaaagc ttttaaaaat
     1021 gtccctgatc ttttttgtaa ttgccggcgc atattttctt atgaattatg tgatagccgg
     1081 tacatttttt ccaaatactt attcagcaaa aatagcatat ttttcagata tagataaaaa
     1141 attcagttat ctcaccgaga aggtatatcc ctttttttcc aacctcagct attctttttt
     1201 tgtcgcagga tttttatttg cagtattaat atccgtatac aacctagtaa aaaagagaac
     1261 atctgaaaat cttttatata ttttattcat cttatttttt ctgtctctgt acatgatcaa
     1321 gcttcccgaa ataaacagat tcgggagata catcatgcct ttgattccat tttttatatt
     1381 attatcaatt tcgggttaca gggaaattct tattatcata aataaatttt taaaaaatgc
     1441 ttttataaca agacttgcat ttataatatt tgcaggtgtg ttattttatc agtctttcaa
     1501 cgcttatgaa actttcaaaa tgttttatgc agaacagtgt aaatacattt atgacagaca
     1561 ggtaaaaact gcaatgtggc tgaaagaaaa tacagatgaa aatgacgtac ttggagttca
     1621 cgatattggt gcgatcggat tttatacagg aaggaaaata attgatgtag ccggacttat
     1681 cactccttat cttaatgaaa gattaactga aaaagattac agtaaaataa tgaaggatta
     1741 tcttcagcag cagggtgttt catatattgc ttttctgagg gaatggtaca gatcaataaa
     1801 tcaggttcca ctttttaaaa ctcctgacta ttctccgcag gaagtaatgg aggtatttga
     1861 atttattccg gattctactt acatcatatc caagaatgca aactatatga tggacaaagc
     1921 cttaatgagt tataaagaaa aagatggaaa cgaaataatt aaagatatga ataacattct
     1981 agagcttgag ccaacttatg ctgaagctta ttatctgagg gcttatggat attctttact
     2041 gaataatgat gaaaaatatc tggaggacct gaaaaattca gtcagatatt ttccaggatt
     2101 aataattgct caaaaaaatc tgggaatgct tcttatggaa aagggtaaat attctgaagc
     2161 agaaagtcat ttaaggaaag cacttgagct tgatcctgca aacaaatcag tacaggaatc
     2221 attaagagtg atcgaagaaa aaattgcgaa gtaaaataat ttatcattaa aaaatctctg
     2281 ttcccgcaga tttacatgct tatcggactc tgcggataat aaaattagat ctataaaaga
     2341 ttattttaaa agtatcatac gttttgtctc aacaaaatcc cccgcatcaa gtctgtaaaa
     2401 ataaatcccg ctggcaaaac cgcttccatc aaactcaact gaatagcttc ccgcactttg
     2461 cttctcattg actaattcag cgacttcatt tccaagtata tcaaatactt tcagcttagc
     2521 attaccggca acttctaaat cgtaattaat aactgttttt ggattaaatg gattgggata
     2581 attctgagac aaagaaaatt tatcagggac aaagttgctt atcaatttta catctgacaa
     2641 tattgtatct ccatagacaa caccatcgat tttacatcca agtaaatatg taaatccgga
     2701 acaatacgga tcaccgctgc atgaataaat ttcaccaaat ccttttgcat attctatatc
     2761 ttgaatgacc aatccaccat gaaaaaactc tttgacttca gtaagtatac caaaaacatt
     2821 ttgattagtg attgacatac acctccttgt aaagattgcc tgaaacaggc aatttatctc
     2881 atcacccgga tttgatgcaa gactgtcaat aattttatca tccggataag aaccgcaacc
     2941 gcttcccgga gaataaagga gaaaattgcc tgaaagagaa tcaattctga catatacatt
     3001 cataccatac cggttcatta gaaaataaat atgaccgttt ataactgtat ctttttgtat
     3061 ataaagttta tgaaatgtcg gaggaaaagg aatcggtgaa aatgttgttt tataatacca
     3121 tgtatttcct gtctttagtg gaaaatatct gactaaggat gtgtcagagg ctattgtttg
     3181 agttgaacaa aataaggtaa ttataaacag aacttttttc ataatgcaat taatattttc
     3241 ttaatataaa aagaataaaa ttcaaaaaat attacctgaa tattgcggtg aaatgtatat
     3301 gttcaattat tgctcaatta tcattaatta tcaataatta cctatactgc tttattaact
     3361 ttaaaaaatt ctcagtttgg aaatgtctaa tggtttgaaa ttacatttca ttttagttaa
     3421 cttgcaagtc tttgtaaaag taaaagctaa cgcggggcta tagctcagtt tggtagagcg
     3481 tctgaatggc attcagaagg tcagcggttc gatcccgctt agctccacaa atacaataaa
     3541 aataataaat tttaaatata caatatgttt gcaatagtta atatcaaagg acatcagtac
     3601 agagttcagg aaaatcaaaa agttttcatt cctaagctga aagatgaagt cggatcaaaa
     3661 gttacattca gtgatgtact tatgttctcg aaagatgata aatcatttga gataggtgct
     3721 ccttcacttg aaatgaaagt cgaagctaca gttctcgatc acgttaaaga tgataaagtc
     3781 atagtcttta agaaaaagag aagaaaaggc tataaagtca gaagaggtca cagacagaat
     3841 tacactcaga tcactataga taagatcagc taagatcagc taagatcagc taagatcagc
     3901 taagatcagc taagatcagt tatatcttca aacaaaattt taaataacat tcaattttaa
     3961 tataaggagt tagaaatggc acacaaaaaa ggactcggaa gttccaagaa cggaagagac
     4021 tcaaactcaa aaagactcgg tgtaaaaaaa ttcggcggtg aagcagtaat agccggtaat
     4081 atccttgtca gacaaagagg tacaaaattt ctcccgggac aaaatgtagg tctcggtaaa
     4141 gattatacaa tattttccct gatagacggt aaagtaaatt ttaccactaa aaaaggtaga
     4201 aagacattaa gcgtacttcc ttatgaaagc gcctgataat ttttaatcac agttttgaaa
     4261 aggttatttt gagaaatcag aataaccttt ttatttttat aaaataaaat aatacagtaa
     4321 atatgaaaat cacagtagtc ggcgcaggaa atgtaggagc tacatgcgca aatgtaatag
     4381 ctcaaaaaga attagcaaac gaagtaatac ttctcgatat taaagaaggt atcgcagaag
     4441 gaaagtcgct cgatatctgg cagacatccg gaattaataa ttatgataca agagtgatcg
     4501 gtgtcacaaa tgattatgaa aaaactgccg gctctgaagt ggttgtaatt acttccggtc
     4561 ttcccagaaa accaggcatg agcagagatg atctcatttc tacaaatgca gcaatcgtaa
     4621 aatccgttac cgaaaatgta gtcaaacatt ctcctgatgc aataattatt gttgtatcta
     4681 atccattaga tgttatgact tactgcgcat ttctttctgc aaaacttcca tcttccaaag
     4741 ttatgggaat ggcaggaata cttgacactg cacgttacag agcatttctt gcagaggcac
     4801 tggattgttc gccaaaagat atttcagcaa tgctgcttgg cggacacgga gacactatgg
     4861 ttccgcttcc aagatatact acagtcagcg gaattccagt tacggaatta atcgatgatg
     4921 aaaagttaaa cgcaattgtg gatcgaacaa aaaaaggcgg tggtgaactc gttgctctca
     4981 tgggaacttc agcatggtat gctccgggtg cagcagcagc tcagatggta gagtcaattg
     5041 tcagagatca gaaaagaatt tttccggtat gcgcatggct tcagggcgag tatggtatga
     5101 atgatattta tttaggcgtg ccggtagtac ttggtaaaaa tggaattgag aaaataattg
     5161 aactgaaact taatgaagat gaaaagacac ttctgactga ttcagcaaaa gctgtaaaag
     5221 aagtcatggg tgttttggat gaattgaata aaaagtaata cgaaagaaat aattctataa
     5281 atcctgttat tgaaattaag taacaggatt ttttttatta caggttataa taaattcagt
     5341 acctgatcct gtggacgtac aaataaaatc atccactaaa gatttaataa taaataatcc
     5401 cctgccgctt tcttttaaaa tattttcatc tgttgtcgga tcaggcacac tgttaattac
     5461 aaaccctgta ccttcatcaa gaattcttat gataattgtt tcatcaccat aaattatttc
     5521 cgtataaact ttttttaacg gatcttcatt attgccgtga acgattgcgt ttatcaaagc
     5581 ttccgaacag gcaatcttta aattgataaa cttttccatt tcaagaccga atttttcatt
     5641 aacactatca agaagatctt caaatttcac gatctcattt ctttcgcttt taagagtaag
     5701 aatttcctta tgcaatttat atatttattt aatcgatttt atactaaaat atgaaagtta
     5761 atttacatat tccataatat attgagttga aaatttacgg aagaattagt ctgggacgga
     5821 ttcagatatt ctatctgatc cagtcccagt atgaatttta tcagtgaatt attattcatg
     5881 tacatctcag caattccaac aggtccgtaa gatttgtaag tatttgctaa aactttttcc
     5941 gcttcatcat aattatacct gtcctgaata taatatttat aacctactcc aaaattcaga
     6001 aaattattta tcatataatt tataccgggc atataaaact gttctgcata aaatgtaatt
     6061 ggctttacag aaaaagaatt attcaagaat tcaccttgtt cgtaaaattt taactcggct
     6121 gcaaaattca atttaaaacc tcttcctaaa ttataagaac ttgaatctct aatactaagc
     6181 tgtctgtatg aaaaactctg cacctgtgag atcagatctt caaattcata tacagtataa
     6241 ttcgcaagta cctggaagaa attattagtg gtaaatcttt caacaggttt gaaactgctg
     6301 tatgaaccaa gtttataaac cctgttagta taattgttcg cacttctttg agaataaata
     6361 taactgagct ttgatactat atattcaaat cttgtttcaa cataaaaatt attaaggtta
     6421 ttaaaattat aggaagctga cacaattgat tctgattcat ccctgtcatc aaaattatcc
     6481 tggaaatctg tatcataaag caagagtgaa gtcgagcctg taaaattaaa agagtttgta
     6541 tttgacaagg gataatatgc attaaaaaga agcgacgtcc ttctgctatt attgttctta
     6601 tttttttcag ctctttcaag ttcacttatc tgagctgctg taagtccttc aggatttatc
     6661 agcgtatgat tttcagatct ttcactgata agtactctaa gctgtgtaaa gagatttcgc
     6721 cagttgtaat tcagattggt ataaacttcc agattatttt ctgtgatctg attatcatat
     6781 gtattttcaa aaaatacatt attcgaagta ggtttgtatt tatattccct cttaattatt
     6841 ctgttcatat aattaccgcc gagcgaaagg attatatcat cagtgaatcc atagttaagc
     6901 atatcgccta tataaaaata attttcatct cggctttcaa tgttgttcaa tacatcataa
     6961 agaagcgcta ctccgggaga cgcagggaaa aaaaagtcat tcttctgatt gtaaaaagta
     7021 agtcttcctg tattatccac ctgctgtgca aatcttttgt aaatttccgt attgatctca
     7081 taattatgat tttgtttttg gtcaaggtct tcgtaaaata aaatgagatt accatttgtg
     7141 atgtaatcgt taagattata attatttgtc tgagtttcaa aaaatccgct gaaccctgaa
     7201 ttgaattcac caatctgatc ctctgttttg tatcctaatt ttgtattaaa aaaaacactg
     7261 ttcatcatac ttgcatccat gtttgcataa aaataattac tgttattctg attggtctct
     7321 acggtcttgt catcagtata aaacttattt tgaaacccga ctcctacatc aatatcttcc
     7381 ttcacatcat aagacatcag caaattcaga ctgttaaaat cacggaaaaa attcttatta
     7441 agctttgaaa catttgatag aaaattattt gttatcaaaa atccgaatct ctcaatgtta
     7501 ttttcataat tcaataatga attaaaattt gccgaattca gatcattata aaaattagtc
     7561 tcaaaaatat tagtgctgta aacagtatca gtcagaatag aaccggactg agacattgca
     7621 gatacaggta tcagcataat taaaaatatt aataaaaaag agtatttaat tatagagatc
     7681 ttcaaaatta aatttctttg attcggaaga gtcttcagaa ttaagatccc tgaaaagttt
     7741 ttcgagatct gtataatttt ccagcgatga aagctcactg gtgtttatgt caagttctat
     7801 tctgccttta tttatcagaa tgatctttgt agaaattttt tcaataatat taagatgatg
     7861 tgagcagtaa aaaatagttt ttcgtttgcc cgcaaggaat cttataaggt catgaaaaac
     7921 gaatatggaa tttgtatcaa gtccgttcag tggttcatca agaaagatta catgaggatc
     7981 atgaatcagt gctgaagtga tcatcacttt ttgtttattg ccttttgaaa gatttccgat
     8041 agagtcattt acaagatcag taaatccaaa catttcggca aatgaattta ttctttctga
     8101 aattacttta tccgtaagat ctctgaccat tcctgtaaag tttagaaatt ccaccggtga
     8161 caatgaatta aaaaggtttg cattttcggg gacatagccg attatttttt ttacttcgac
     8221 gggatctttt tttatgtcaa gatcatttat cagaacactg cctttgttaa aatcgagtat
     8281 accggttaat atttttacag ttgtagattt acctgcgccg ttagtgccga tgtatccgca
     8341 tatctcgccc ttttgaattt caaaagatac atcatttaca gccgtgaaat catcaaactt
     8401 ttttgtaaga tctttaacta agatcatgca cttataaatt taaattcagt taaatatttc
     8461 agcaaaaaaa actttcatat agtcccatga tctgatatca gctttctcat tataagctga
     8521 tccttttgaa ggatcattgc cggcattttt atctgtgaag gaatgtactg ctccactgaa
     8581 ttcagccaaa acataatcta cgccgccatc ttccatttct tttttaaatg caataacatc
     8641 ctcctcagga acattaggat ctatagctcc gtgcataatc attacttttg ctttgatatt
     8701 ttttgcttca tccggatttc ctgacttaag tgaaccatga aaactcacga caccttttat
     8761 atctgcgcca cttctcgcca gttctaaaac tccgccgccg ccgaaacaat atccaatggc
     8821 tgcaatatta tttttattta cattatcaat tttcatcatt tcattcagac ctgcatttac
     8881 tctgagcttg aataaatctc tgtcagcata atattttccg gcttctttac ctgcttcttc
     8941 acgggtctga gggcggttgt cctttccgta aacatcaacg gcaaaggcaa gatagccaag
     9001 ctctgccagc attcgtgctc gcattttttc ataatccccg agacctttcc actgatgaac
     9061 tattaaaatc cccggcattg tacctttgat ggaattatca taagcgataa agccttgata
     9121 agtcaaaccg tctatctgat aatcaaaatc ttcttcgatg atctctgctt ttaaattcat
     9181 tgaaataatt gcaaataaaa ataaaaaaaa taaattaata atatacttca tgatgtgtgc
     9241 tgtttgattt tgaaaaatga tttgttaatt ataagggaaa tgaattgtaa ttagaatata
     9301 aaaatgtgag ataatgattt gaaataattt caattgttcg aaattacaaa gccaatatgt
     9361 cttaaaaata tagttcaaat aaaaattaat gtgtttatat tgattcctaa taatgcagga
     9421 ttatcaggaa aatatagtta gtagcggaca aagtgaatca gatcttgaaa agccaaagat
     9481 cctgtcttca ttttttttca gcggagtatt tgtactgctg ctctggataa ttcagtttct
     9541 tcaatggtct ttggcaaaag agtttgccac atttggagtg ctgccgagga ctctgaccgg
     9601 tctgaaagga atattaaccg cacctctaat acatgcagac ttttcacatc ttatatcaaa
     9661 ttctttttca ctgtttctat tgctgtttgg aatactttat ttctacaggt cctcagcagt
     9721 aaaagtattt ataataatct atttagtaga tggaattatg gtctggctgt ttgcgagaat
     9781 gtcttaccat atcggagcaa gcggtcttgt atacggattt gctgcattct tatttttcag
     9841 cggactgttc agaaaagaca gaagttcaat ggcgttatca ctcttggttg tatttctcta
     9901 cggaggaatg gtttggggag tgttgccggt caagcctgaa atttcatttg aatcacattt
     9961 attcggagct cttacgggaa tagtctgcgc atttattttc agaaaaaatg atccacccga
    10021 aaaatatgaa tgggaagaag aagattacga agaagatgat gattatgatg atggcaaaga
    10081 gcctgaagat gtggagattg atgaagatgc tgaggaagat gattttagga ggttgtcctg
    10141 aacagttatg cagattgcca cgaaaactcg aagactcaaa ggtttaaaaa aataagaaaa
    10201 atcatttcct cagagtccga agaacaagtg actctgcgga gaaagagagt attattttta
    10261 attctaagct ttgaggcttg gagtcttaga ggtgttgcag cactaaagtg tttcatgtaa
    10321 aggcaaatta aaagtccata tcaccagaat catttccgtc attgttaccc tgtctttttt
    10381 tctttctttc cttacttttg ctatcagtac caaacttata agttagtgac agtgtagcca
    10441 ttctggaatc tcttgccctt gtgaactcct gagtatataa tgaagtcgta gatgatccgg
    10501 aaaattttgt ggtattaaaa acatccgata ctcttaaatt cagatttaac ttatcattta
    10561 aaaagtcttt acttacagct acatcaaatg aactgacagg atctattgta ccctgaggag
    10621 taatggcttt tcctgaataa aaataagtaa gctgtaatcc ggattcaaac ggcagtgcaa
    10681 aattagtaaa taatctgcct gaaaaagtag tattggaatt attgccgagt gtatctgaat
    10741 ttatgtcctg ataaaaataa ctgacgctgc cgtttacagt aatactattt gcaaacattc
    10801 catttaaaag aagctctgca ccatatgacc tttcagtatt tgcatttata ggtacattta
    10861 aaataacatt actgtctatc agtacacttg tccttgtaat tacatcactt atatttttgt
    10921 aaaagagaga tggaattacg gaaaactcat tgaagaactt tgcataatta aattcaatgg
    10981 aattaatata ttcaggattt aggtccggat ttccttttga taatagcacg ggactgaata
    11041 ctctcgttac aggtgacagc tctctgtatc ccggacgtct gatctttcgg gtatagtttg
    11101 cacctatctc atcagataaa gaaagcttat atgaaatacc cgcactcgga aaaatgtcaa
    11161 atatatttcg tgatgtattt gatgaagaca atatctgatc aagattgtaa ttccagtttt
    11221 cacctcttat tcctaaatta taattaagat caccagcctg acccgtataa ataagataag
    11281 cagctccgac tccgtcttca aaactgaaat catcagtaag tgctgtatca ataagatata
    11341 cttttgatac agtatcctga gtataataat aattatctgt atttgtattt ctcacattat
    11401 atttaattcc ggattcaaat cccatattct cgctgaactt attcacgtag tctatttgtc
    11461 cgttgaattc tttatttatc tcactgccat ttacaatcga aaaaattgac tgaagattag
    11521 caggaaataa aaaatcaatt tgcttgtttt cattttcata ttctttatca gtagcaaaac
    11581 ttaattcacc tgtaagatta tgtccttttt tacttatcgg tttaaaataa tttaaaccaa
    11641 gagaatatcc gttacttgtt tctgtttcat tgtttagagc tttataatct tgaaaaagat
    11701 tattttgatt atcataagtt gttaaattat ctgtatctcc cctgtttctc tctctttctt
    11761 catatcttgc attgaatgtg agcacatcag ctttggtagg ataaaattca agttctactc
    11821 tggcattatt tccatttgac ctgaatcttc cattcgaata ctgatcagtc tgagcttctt
    11881 ctgtactaaa aaaattctgt ctgttaattg cggaattaaa aaatcgtgtt gctattctgc
    11941 catcataata tcctgaaact ttgtaatctt tatttttata atttagatta aatccggtat
    12001 tgtaattatc ctgcgttcct gtgctgagtt caagctgacc gttaattccg ctgtcatcat
    12061 attcttttaa taccagattt attacacctg tcgaaccttc tgcgtcatat ttggctgaag
    12121 gatttgtaat cacttccact gagctgatct gatcagccgg cagttgctca agaattcttg
    12181 taatgcttcc agtaacaggt cttccgttaa taaaatattt aatattagaa ccaccgcgca
    12241 ggcttacatt attgtctata tcgactgtta cggatggtag atttttcagt atatctactg
    12301 cacttccgcc ttttacactc atgctttctt ccacattaaa tatctttttt tcgccctgca
    12361 tttccatata tggtttttca ccttctacaa cgatctcatc tgtctccgta cctgaattca
    12421 ggaagattgt atctgttaca atatctttag cagaagcatt taagacatct gcatctctca
    12481 tgtttttatt atatccggca agggatacat gcagtctgta ttttccctga gcgacatcgt
    12541 taattataaa aaatcctttg gcgtctgtct ctgcaccgcc tgccagtgtt gaatctcttt
    12601 ttctgaaaag ctgtactgtt gcgctttcca tcggggtatt gctaagtttg tcaaatacat
    12661 atcctgagac tgaaccgtca tcgaattcag gttttaaatt atttcccttt cgctgattgt
    12721 tatcaggctc tgtttgggaa tatgctgaag tgaaggaaag tattaaaaat ataaaaatta
    12781 gaattctcat gtttgtcaaa tatgtttaaa tttgtaatca aaaaatcagg tggtaactgt
    12841 cttttattat aaaacggaaa atcaggatgt gattattttt aagaaaattt atttcataaa
    12901 actcactttt taaaaactat attaaattac agttttcagt aatatttttt ttgacaattg
    12961 atatttgaaa aaggtttaaa caaataaaat taattatatc tcaaatacct gacctttcac
    13021 aggtatttca atgtctttaa aattttgaaa ttttaatccg tccttaaatg cttcctgttg
    13081 atcaatttca ccgtgtacta agaagatctt ctgcaattta tttctatcga acttatcaaa
    13141 atattctaac agctcatccc tgtctgcatg cgcggaaaaa gaattcagaa ctacaatttt
    13201 tgaatgaaca aaatattcat caccgaaaat tcttatcgat gcattttctt cttctcttga
    13261 ttcaattaat cttcgaccca atgtatgctc tgccatataa ccgacaatca gaattgtaga
    13321 tttgggatct tcgatgttat tcttaagatg atgaagaatc ctgcctgact ctgccattcc
    13381 agatgctgaa attatcatac acggaccggt aacactgtta agactctttg attcctcaac
    13441 atcctgtaca tatctgatct gaggcaattc aaatacttct ttccctctga tgatcatatc
    13501 agaaaattct ttgtcataac attccggatg cattttaaaa atatcagtag cttttgtcga
    13561 aagcggactg tctatgaaga tcggaaattt ttcgatcagt ccttcattaa ataaaactga
    13621 cattgaataa attatctctt gtgtcctgcc tacactgaat gcaggaataa taatctttcc
    13681 gcctctcgac acagcttcct tcagtacagc aagtaattgc tgatccatca tgtttgcttt
    13741 gtcatgaatt ctccctccgt atgtcgattc ggaaataata aaatccacat cacctataaa
    13801 ttcaggatcc tttaaaatcg gaagtccggg tctgccaatg tcacctgaaa atgcaaattt
    13861 aaattttctg ccattctcct ttatagtgag aaccatcatc gccgaaccga gtatatgtcc
    13921 cgcatcaaaa aattccactt ctactgcttc acaaagtccg tctatacaaa aagtctttcc
    13981 ataagaaact gtcttaaaac tcttcagcac attcctcgca tcatcaattt tatacaaagg
    14041 tttaaacggc gactgatttt tttttgcccg tcttttattt acatactctg tatctttttc
    14101 ctgaatatgc gcactgtcga gaagcattat cgagcaaaga tctctcgtcg cagatgtaca
    14161 gtaaatagaa cctttgaatc cttttgaatg aagtgtaggc agatttcctg aatgatctat
    14221 gtgcgcatgt gaaagtacga cacagtgaag ttcctctgga tcaaatagaa aagttctgtt
    14281 aatgtcataa gcatcttgcc ttttaccctg aaacagaccg cagtccaaaa gtatcttttt
    14341 tccgttgatc tcaagtaaat gctgagaacc tgtaacagtc cggtcagctc cgcagaattt
    14401 gattttcata aatgtatttt aataattaaa aaatatttac tgttctgaat taaatttatg
    14461 ttttgtaaca aaaggatttt cctttatgaa aaacctgtaa ggaaattcca ctcctttatt
    14521 aagacctatc cttcgtgatg taattatttc ggatcttttg atacgatcgt tttcagcaat
    14581 aaagatttca ctgtctgtat ttgtaatatc agttctgttt aaactcagat ctacattcat
    14641 tgccatgcat aatttagatg gaccgtttgt aagctcaata tcatttttta cattaggtct
    14701 gaactttttc atgatctcaa ttccttccac aggctcacct gctcttatga gaattgcatt
    14761 accggttcct tctttaccgc acacagcatt aaaacaataa taacttccat aaataaaata
    14821 cacataagcc tttccctccg gaccatatat aacactgttt ctttttgtaa ctcttccaaa
    14881 tgcatgacat gcaggatcat gatctccgaa gtaagcttcc gtctcgacta ttttgaccgc
    14941 agcaaattta ttcccaaatt ttctgatgat cagtttgccg agaaggtctt tggctacaat
    15001 tacagcatct ttcagataaa attctttgct taaagattta acatcatata aattcatact
    15061 gagctatata caattttatt tggtaaaaat tcgtaaaggt aatatatgga aaatcgattt
    15121 tattgaatat ggatttagaa tggatgttgt tcgatttgga atttattttc tccggctgtt
    15181 ggtgaacaca ccgaaatgtc gaagccagca gtaaacctct cccagtctcc cctttgaaaa
    15241 aaaggggaag agcctgaact tttgaatttt aagtttattg ttttcaaata tgattttgcg
    15301 agcgaagcga agcaatctcc gaattcacga caagaatttc ttgtcattac tcatataaaa
    15361 tttggagatt gctttgccgg aaaatcacag gctcgcaaag acatttattt tttgtgattg
    15421 cttcatccga agagcgcggg ttagctaagt catttgttta gataaaattc ttaattccta
    15481 acttttaatt cttaatttat tttataagca tcatacgttt cgtcccggaa aatttacctg
    15541 ctgtgagttt ataaaaataa actccgctgg aaaaattatt tccgttgaaa acaacagaat
    15601 aactgccggg ctgctgatat tgatttacaa gtgttgctat ctcttttcct gaaatgtcaa
    15661 aaattttcaa attaacaatg tctgcattcg gaattttgta attgatgttt gttacaggat
    15721 taaacggatt tggataattc tgattcaaaa cgaaattatc cggaagggaa gttactgaat
    15781 tattaattcc aataactaca tcagtaaagt aattatcata atattctttt gcagtaattg
    15841 cagcttcacg caattttgtt atgctgttaa gattgctgct gccttttgct atcacctgcg
    15901 ccatcacaat gatctgcgta tcccccggat taattgtcat cggtccaaag ccctgataaa
    15961 acctcatgtc tcctgaattg ctttttatcc agccatttcc cgtaacagga tctcctgagt
    16021 acatgaattt tgtcggttga tttgtcactg gatttatgtg cggattgccg tcatttttaa
    16081 atccgcggat tgcattatat gtctgagtat aatttgaagg ttcaaatgaa tcatcatgat
    16141 atatcacagt tgaattcaat gacatttctt tgtaaccgat ctttaaattt ttatttttac
    16201 cttcataata aaaaactgta tcgtttgaat ttcctgtgta aacaagcgga gctcggacaa
    16261 ttaaatttcc cactgcagga ggtccatttc cataaatgta atctgtttgc gtgaaattat
    16321 aagtataagc cagtcttaaa ttagtatcaa ttccttcagc gtcattttga ctttcaccaa
    16381 gatcatcatc cgaataaaaa ttaaaataag cttcattcca aacttcatta cttctgttaa
    16441 tgatcctgta ttcctgataa atcacattct ttaatttttc aggctgatta aaggaaaaat
    16501 taatctgctt aatgtcagct tttagtggag ctgtacttgt attataataa ttatgtgatt
    16561 ggggataact gtctgtataa gaataaaaca ttgtctgatt tccgtagtca accggtttcc
    16621 atgtcatcat cgaggaatca aaatatacag gcgctccctg atcggaattt ccaagcgctg
    16681 aattcggcca ttttattctg tctgtatcat taacattatg tatcaattta taaattttat
    16741 attcgggtgt tccatttccg tgaggtgtgc cgctctgagc agtatatcct ggtaaatatt
    16801 ctgaagaata agatacagct gaaactctaa tttcattatt taccaaagca ccgagataat
    16861 gtccggatga ataacgggca tatttatttt caccttttgg ccattgaaaa cctccatcgc
    16921 ctaatggatg atgattaaag ataccgttgt ttttgaacca agcactaatg ttattcgaat
    16981 ttaactcatt ggtgcctgaa gacacataaa aactttttgt gtaaattatt aatccattat
    17041 ctccgacagc aaagcagttg tcgatatcag attttactat agatctcaga ttggtgttcg
    17101 tgtttgtagg gattggctga aagaatgaaa ggattgtgcc ggatgcaccg catacaatat
    17161 cataatttaa attacgatca tacagattat tgacagtgtt tgtatttata atattaatat
    17221 tgttattttg tatttgaaat gctactccgc tgtctccgat aacaatacta gaataagaga
    17281 tctgattcag atcattcgaa atgttactgt ttatccttac ccagtttgtt ccgctgtttg
    17341 ttgatcttaa gatcaatcct gaatttccaa ctattgtaaa tctggtatta ttttcaattg
    17401 ctattgaatt cagatcagca gtatcgatct gtaccgcttc ccagattcca ttgatcagtt
    17461 ttaataacgt accgttttct cccacagcga cagcactcat aggtacagca ttagaattag
    17521 taaattttaa atctttaata gtagaagcag ttgcagaggg aatagtgaac cagttgaaac
    17581 ctccgtctgt agtttttata atcactccgc cttcacctgc acaatatcct gtatctccaa
    17641 aattatctct gaagccgatg gtgtatagat ctgcagttgt attgctgtta attttttgcc
    17701 atgatgtacc tgaatttata ctgcgcagaa tagtaccatt atctcctata acaaaaagag
    17761 aatgggacat attatatcct tttaaatata ttttattgag atcatttgaa gtgccgctgt
    17821 tttgaattgt ccagacaggt tgggaaatta tttttgtgct gagaactaaa ataaataata
    17881 ttgacaagat ctttaaagtt ttaaaatctt tacccgcaat ttttttcatt attcttttca
    17941 taatttagtt taagagctga aatattttta atttaattta atacatttta atatttctta
    18001 gtttagaaag caaagaaata ttattcagtt tgtgcggcaa aaatggaata aaatattgat
    18061 tttttctgag gtattactgc aatattgttt aatatcagtc tgatttcgca ctttttcacc
    18121 ggaaattctt cggaacgaaa aggctgtttg tggattaatt gaaaagctga attttattta
    18181 aatggtatta ttttatgttt ttagaaaatg attttgtgag cgaaatgaag ctacctcgaa
    18241 tattatgcca aacctaattg tagtttataa ttaaattttt aatagaaatt atattctttt
    18301 aaattacagt aatgtataaa attcggattg cttcacccga atgaattctg gttcgcaaag
    18361 tcaattgtgt ataaaaaatc ccgcagagaa attaacaatc tccgcgggaa aactttcaga
    18421 tcaaaaattc ttaattccta ttttttaatt cttgattatt taataagcat catacgtttc
    18481 gtttcactaa actcaccggc attcagttta taaaaataaa ctccgctgga aaaattgctt
    18541 ccgtcgaaat ctacagaata cgtacctgca ttttgtttct tatttaccaa tactgcaact
    18601 tcatttccaa gtacatcata tacagttaac tttgcattac ctgtaacttg taattcgtaa
    18661 cttatgactg tctttggatt aaaggggttt ggataatttt gtgaaagaga atatttttca
    18721 ggaatttcat ttgatatatt attaacactg acaagtccgt caggtaaata tttgttaata
    18781 tactgaaagt tagctcctgt cagaacagtt ctgataagac tacctgatgc gctgtcgatc
    18841 tcatgaacac ctgaaccatt tgtcacaaga taatgaccat tgccaagaag ataagctcct
    18901 cttaatcccg tagctccggt taaagatctc atgtaattac cggaagaatc aaaaagtatg
    18961 agcccggatg gagtgctaaa tccgcagact gccagattcc cgttgtcatt tcttatcaac
    19021 tgctgagcga aagtaatgtt tggactgtta ataaaatttg aaatgaatgt tccgttgtaa
    19081 tcataactga tcacatcatt tccaccggat gaatttgtaa tcaggatgtc attacttctg
    19141 taatgaatat caaacggact gtttaatgtc cctgtgggta tgaaactacc tatgggattt
    19201 cctcccaggt caaattcctg aatagtattc tgatttgctc cactgccgac tgttgcaagt
    19261 aaattataat ttggtctgaa tgcaatacct ctcatattat ctactataga tgtattcact
    19321 ccacttgcag gtgcaaaaaa tccgacatat aacccgttcg tatcatactt ctgcaccaga
    19381 tctgaaatct ggtctgagac atagacgaaa ttattcggtg cctgcagtgc catcttagga
    19441 ctctgaagat ttggattatt tgaatgtata aatgctgtgt caattagatc tcctgtaacc
    19501 gaattaaaag ctgagaccct gtcaaatgtc cagtcaggaa tcagtaggat cggctggttt
    19561 ccacttactt caggatttag aatgatttta taagtatcac ctgaattatc tactatatta
    19621 ttcttcgaat aaattataag tgaagtcgaa aggtcaaagt ttttttcaat ctcaacgaag
    19681 aacatcacat aagactgaga atatatatca gcagaatttg taattactga gtaaacaact
    19741 ttgccgtcct cgataccaat tgcatttatt actgttttat tttttgtgag gattgtaacg
    19801 ggtaaatgat attgcatggc aagttgctta gctgagataa cagattgagc atctctttca
    19861 gcattagtaa tgagttcggt aaaactgatc tttggaccgg caaaagaaac tgaactaaaa
    19921 gaaagaaaca ataacgcaag tgtaaaaagt aattttggtt tcattttgtt aaatttaatt
    19981 ttaaaaaaag ttggatctat attaagaaag ttcaaaatta gctttccgaa ttcagactaa
    20041 ctttgcaaat ataattaatt tttacaaatt aatcgtgaca tgttttaaat gaagattttt
    20101 ttatttccgt atttattaca atctaaagac atcttatcaa cagtaaataa ttttcagtgt
    20161 gtaaatttta tatgtcaatt caaaaattga attacatctt taaatatgat ttcatatttt
    20221 taatccaatt taaatttcga tttaaatttc aattccccta ttagaaattt tttatatact
    20281 ttgttaaatt aacagatata atttataaca agaaattttt attaatcaaa aataaatata
    20341 atgaaaaaaa acctttctga atttataggt acattctggt tagttctggg cggttgcgga
    20401 agtgcaatgc tggctgcggg atttccggat atgggaattg gctttgcagg agtttcacta
    20461 gcttttggat taacagtatt aacaattatt tatgcactgg cacatgtatc cggtgcacat
    20521 cttaatccag ctgtttccat tggattatgg gtcggcggaa gatttgaagc aaaagaaatt
    20581 ttaccgtatg ttatttctca gatactggga gggctagcag gagccggtgt tttatatgtt
    20641 atagtaacag gaaatggttc acagatcggt acttttgctg cgaacggata tggagagcat
    20701 tccccgggcg gatataatct gatgtctgca ttcattactg agactgtaat gacatttatg
    20761 tttttgatag taatactggg atctatcgat aaaaaagcat ctcccggatt tgctggagtt
    20821 tccattggac tttgtttaac tttgatacat ctcatcagta ttcccgttac caatacatca
    20881 gtaaacccag caagaagtat tagtcaggca ttatttgtag gcggatgggc aatagaacag
    20941 ctatggctat ttattttggc accaataatc ggcgcagttt tagcaggatt agtatataaa
    21001 atttttaaaa cttctgagta atcataacag aaagattatt gatttaagat taacataaaa
    21061 gaccggatca tctgaccggt ctttttattc ccaaaagatt gttacggctg aaagattttt
    21121 tccatttata atttctaaat tccgctaagc tgttataaac cgttaaaacg gttaaaactt
    21181 catatcacaa taacccaaca tattctattt agtcattatt caaaaaagtt ttacagctaa
    21241 ataatttttt ccattttcaa tttctaaatt cccctccgct gctataaacc gttaaaacgg
    21301 tttatagcac ccaattaaca ctctgttaca tttattatta ttataacaca taattttcaa
    21361 ccccaacctc caaatacccg aactgtagaa atattgctga attaaagaca ctaatgctgt
    21421 cagcaaatgt cataaaaaaa ttacttttta acataacatg ttttatcagt actgcttaga
    21481 aaatctttac ctcagccaaa ttgtttgttt aaatttttta aaatattatt aacaaaaagc
    21541 ccgctccgaa aacaaaaccg gaacggtcta gagtatatta aataaatccc aattggaaat
    21601 cctgaattta tttaaccgat gtgtgggtaa ctttttgtat cgtattgatt gcattgtttt
    21661 cagcgtattt catatcagtc agatcgacta atccgtcagc aattagatct gagacgattc
    21721 aaatttgcat cctgatatct gccttctgat gtgaatggtg aaggaattac tgatcttgtt
    21781 catgcagagt ttgctgataa taacgggttt aactttgcag gtaaaattac tccgaaggag
    21841 caattactaa ttacaaatgt caaactaaag tcggcaaaaa gcaaatatgc aattttaaaa
    21901 cagcaattta aaatttggag taaagataag ttaataaaat aaaagcagcc gcaggcttaa
    21961 tgctgaggtt tttttttaca gtaaaatttt tttaaattta aattggaatt ttaaggactt
    22021 tactaagtga ttagtaaaat tattattctg attttatttt gattctttta taatttcaat
    22081 catcggaatt ttttttattt tgctgtcgag ccagaaaata atattaaatg catcgaatgc
    22141 atgtttttcc ataggatcct ttaacagcac gttcagctca tctttcattt ctttaaacat
    22201 ttctatcatc tctttgtcag tttttacgcg gaaagatttc ctcagatatt tctgaattat
    22261 attttcatat atatacactt tcttcttcat gctgataaaa tgatatgctg atttcaaagc
    22321 atattccatt gaatcgatgt aacctaattc atagtatgaa ataagctgaa taatctttgc
    22381 atagcactga taatcttctg atagatccgt ccttcctaaa ttaaaaattt tactgttcca
    22441 gtaaatacaa ttttcgaact cttcccgaat gaaattcgat gcgcttagaa agtagtaaag
    22501 tattattctc tgatatatag ttatcttatc ttcgtacaat atgatatttt tttgtatatc
    22561 cgactctgct tttactaatt ttgattcatt aaaagattcc atataaatag aaattctgag
    22621 gatggaagag gaataaaata taaaaacctt attaacttca gtaacagatc cggaatgaat
    22681 tattaaaaca ttctccattt taacaacagt tttttcagct tcatcatatt tacggttcct
    22741 tatttcgcaa ttggccagat tattcagtga aaaaatataa ttatctaatt tacttttcaa
    22801 cttgaccata ttattctcac aaagctcgac agcagatttt gcgcatttgt atgcatcttc
    22861 aaatttttcc atagtcagat aatactgcag ccataaattg taaaaaatat atttggctaa
    22921 gaatgacttt gcatttttaa tgtcactcag gagagtgctt tcgaatattt ttttaaactc
    22981 aatctgcttt tctttattcc ttaccctgcc tgaaccttca cttctcagga aaatcccgac
    23041 gttttcattt aatgtgtaat aatcaagaag atttctattc aactcaagca gattatattg
    23101 attttcaaag atctcctttg acagaattgt gtattcctct actgagcact tatatttttt
    23161 aataagtctt tcattgttta agatctcata ctgactgtta taaatttctt tttcgactgc
    23221 tgtcttcttt gctttctgta aaagttttaa gctctgatca atgaggaact tgtcggaaag
    23281 tatttcagac ttaataagta aatttctgag tgagaatttg tcattatcat ctttatggaa
    23341 ataacacaat gaattaagaa ttgagttata taaaaaattt ttgtggaagg gaaggttctt
    23401 aataaactta cctgaatagc ttccattttt gatcctactt tcatcatagt cgattccgga
    23461 ttcaatttgc tttgcaattt cattaaacaa tttcagataa ttgttatcag attcttcaat
    23521 tagcaaaaga aatttttttt tgaagaatga tttttctttc cccgacattg attttatcag
    23581 caggaaaata tcgtccgaag gtttcattct gactaatgaa tttaattgta gatttataca
    23641 aaaatacgaa atttctttta taattaatca tttcacatcc tgatttaata atcaacactt
    23701 cattctaact aataataaaa caaattcgat ttttaaaaat aagcctgaag aaatatattt
    23761 gtaaagaatt ttaaaagatt atccttcatc ccgaatctga attttataag ttctccaaaa
    23821 tatttactgt cagggatgat ggagggtgga aggcaaagct ccttaattta ggagcttgcc
    23881 ggatacccct ttttaaaatt atttatattt taatcattaa accaacaagg agttaaaaat
    23941 gaaaaaaaca attctatccc tgcaaatcat ttttgtaatt acaattttct gcggatcttc
    24001 tgctaatgct cagatgtact ggaatcaggc cggagatttt cccggaacgg tcggcagcca
    24061 cattgccgtt ccaaattctt catcattaga cttaacgagt ggattcacac tagaagcatt
    24121 gatcaatccg gcggcggctt cctttattgc caaaggaata atatcaaaaa gcacaggact
    24181 caatatcaga tatgcaatga gactggtatc aggaagaatt tttatattaa caaattcagc
    24241 aaatagatta gtaagccgcg catcgaatcc tataccattg aacaaatgga ctcatgtagc
    24301 tgcgacttta agttcaacag gagtatatca gatttacatc aacggaatac tcgatacttc
    24361 caattcgctt ccgggaactt tacccacggt aacttcggat tcgctcctta tcggaagcac
    24421 cggaccatca acggaatttt ttggaaagat agacgaggta aggatatgga atacaaatct
    24481 ttcaccatta gagatctttt ttttaaggaa aaccacttta ggtgtaagcg gaacattcta
    24541 ttccaaactg gtcctctcaa taccatttca aaataatacc ggggcaggca caatattttc
    24601 agcaatggat cattccgata aaataaatca cggctttatt agaaatgtaa ctgcgtttga
    24661 tcttaaagac agaccatcag gaattcacca tttgtcagat tgtgtaaatc tcggtccgtc
    24721 aggattttta accgcatctg ataatcccgc agtaagtcct gtgacaaaat taactattga
    24781 atgctggatt tttccttcga cacaaaatta cgccattgtt tataaaggtc cggcattcag
    24841 cagcaatccg gattacggac ttaaagtgat atcaggaaaa ttagtcggtt atattaacaa
    24901 tacgcaaatt aattcggagg attctgtaaa gaccgaacga tggtcgcatg ttgcctttac
    24961 atattttggg gcgaccggca gatttgaatt ttttgtgaac ggaaagcagg gaacaacagg
    25021 aaacattgct cccgcaaata ttaataacgg ttctgattct ttatttgtcg gagcatttcc
    25081 cttaacaagc actctaaacg gatatattga cgagttgaga atcacaagtg aactgaaaac
    25141 actgcatgac ataaacaagc aaatgttcag ttcagtaaac gaatcgaatg acaatgatgc
    25201 agtaacaaac gttgcatata atctggacgg ctcaactctt cccagtacag atgatggtcc
    25261 gagattgaat ttaagaggat cctcaatttt tatttttaac acttctcaat tttcttcagc
    25321 tataccacat tcgccgttaa ttaataattc ttcgggacaa tttcaaagcg gatattatct
    25381 taatatgcca cagaaaagaa tccctgcttc aggaacagcc ggaacaataa gagatacaat
    25441 tgaaatttta tcatccgaaa cgatctccga tctgaatatt tatgttgcaa taaatcacat
    25501 gagggaagaa aatttaagaa taacaatcac aagcccgctt ggtgcttcga cagatttatt
    25561 cgcgaatact tccctgatgg acagtaataa aaatcttgtt actatattcg attcggacaa
    25621 tgacagctca ttgataagta acagatatgt cagcttcggt ccgggtataa aaccattgtt
    25681 cgacattgat gcaatatttt ccggaagcaa ttcaaaaggt aaatggatac tcaccataat
    25741 tgatgaaggc gcagcagata caggaattct gatcagctgg ggactgcaga taaatgaaca
    25801 taataattta ccttataatc ttgaatgctc atcacttata gaagggtttt ataattcatc
    25861 ttcaaactct atgaccgctg atacagtaag atatttcctg cgcagcatca cttcaccata
    25921 ttcaataatt gattcatcaa aggcaaaagt gaattcatca ggatcagtaa atgcatcatt
    25981 tacaaaagca cagccggaga caaattactt tcttgccgtt aaacacagaa attccataga
    26041 gacctggact tcgacggtga taaaattcaa tcagtttaca aaacagtctt catataactt
    26101 cactgatctg gctacaaaag cattcggaaa taatatgaaa caggttgaca gcagtccggt
    26161 ccgatttgca gtttacagcg gagatgtaaa tcaggacgga acagtcgatc tcagcgacgg
    26221 tagtcttatt gataatgacg catttaattt tgctggagga tatctgccga cagatgtgaa
    26281 tggtgatgaa gtctcggatc tggcagatgc agtgtttgca gataataatg gatttaactt
    26341 tgtaggaaaa attacacctt aattaattaa aagatatatt ttacaaacag aaatataaat
    26401 ttcatgcagc cgcaggattt ttcctgcggt ttttaataaa tttttttcaa aataagaata
    26461 ctactataag caattaaata gttttgatat aagtgaataa aataaataga ttaataaaaa
    26521 acaaacagaa ctgttttata ttacagcatt actaaacatg tcactgatga atcgggaaat
    26581 atatttgctg cgggaaatta tacaacggaa ccggagatgg taatgacact gcacattcag
    26641 ttgctctcga taattccgga aatgtttttt tgcagatgaa agtaaaggag ttaataccga
    26701 cagagatttt gtgccggcaa tattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nnnnttgcag atgaaagtaa aggagttaat accgacagag attttgtgcc ggcaatattt
    26881 ttatcacagg gacgacaaca ggattttctt catcttatga tttcatgaca aaaaagtatt
    26941 cttctccagg tgatctttta tggtcgaaga aatataacgg tcagaagtaa ggtattttac
    27001 aaatttaatt tctacttcac ctgccgggaa ttccaatctg acaaactggc tgatttaaaa
    27061 tgaaaagtat ttttaaaatt tcagattatt tcagacttta aattttttta aaatgtaaaa
    27121 aataaaaaac ccgttccgga aaacagtacc ggaacgggct taagtatatt aaataaattc
    27181 cgattggaaa tctgaattta tttaatagat gagttatggg gtaactttct gtatcgtatt
    27241 gaatgcatta ttatcagcgt atgtcatatc agtcagatct acaagtccgt cagcatttaa
    27301 gtctgatgga acatatcccg taatgaaatt aaaaatatca ttatcgataa ctgctatatc
    27361 tgacagatct ataattccat cctgagtcac atcaccactg taaataccaa aagttaacgg
    27421 agaattatca acctgctgca tattgtttcc aaatgcttta cttgcaagat cagtgaaatc
    27481 ataattcata gtagtaccgg atataaatac ttctccgcct gacttgctcc atgtctcaat
    27541 agtgtttctg tgttctataa caatataata attacctgaa ggcagatcaa aagtaaatgc
    27601 tcctgtgaaa gtcagtgaat ctacaactgc tacagaagta tcaagaatat tatatggtga
    27661 attgtcagaa cgaagataga ccgtcattgt atcgcttaaa tttaactgct gcaatccgtt
    27721 atagaatgct tcaggcaata ctttgatatt aatagttgag ttcggaatac cgttacagga
    27781 tcctggaacc ggttggaatc caataacacc accctgatca tcagtaccgc ttgtcagata
    27841 tggtacataa tttactgctc ctgtgatctg tgcagatata gttacattat ccgtagcacc
    27901 ataccagtta caggttgaat tctgtatcac tcctgactga ttatccagag caattgtatt
    27961 tgtatttaaa tcattatcat taacagtagc atttagaact gcacctgcat ctttatagaa
    28021 tataacacct gttgcccatc cgttaacttt attttcagtt acgtttgcat taagtgtctc
    28081 tgttccgaga gcatccattt caataccaac accatttgta ctatcgctga caagtacgtt
    28141 cctgtagatt tcagtattta gtacaagatt atcaagaatg tttccgcttc tatttgatga
    28201 agtagataca acatctaacg gttctgcagt cacatgcgga attccacccg gatctgcaat
    28261 gattccgtaa taaacatttg taccattatt tactgctgtt gaattaataa tattttcact
    28321 tattaaacca ccaacatcaa tgtaatatat tccaacctga caacctgtga gagtattacg
    28381 tgatgttgta tcaattccag aaggttggaa cagaagtact ccgcaagata ctgctgtacc
    28441 tggtgtataa gacagaccgc tgataatatt atctctgacg gaaccgtttg ctccggagct
    28501 tatctgaact ccgttttgag ctatgaaagc aacaggacct gcaccggtaa ttgtattgct
    28561 gtcaactctt accgacagat ctgctccggt taatactgtt gcatttttct ggaaatcata
    28621 gatattatta cttactacat taagttgtct cggattaccg ttatcagaga atgcataaat
    28681 accaactcct gcctgcgctc cattaatcgg agtatttctt atagatttga tctcacagcc
    28741 tgtgactgta ccacctgcat ttctgtatgc aataccaatg aatctgttat ttgcatttcc
    28801 ttttccagca ccgtcaactg tcaaattttt aatgacaaca tcagatgtat catgaacata
    28861 gatcacaggt ttattatttg gacctgttga gaaggaaagt ggaagtatat ttggagacaa
    28921 tattgtagtc gaaccaatac cctgtccttc aagagttact gctttatgaa tctctacctg
    28981 ctcagtaaat gtatgagcac ttacctgaag taaatgattt gctgaagcat catttacgga
    29041 acgctggatc tgtccgtatt tgatatttgt ataagcagta ttatctatcc acttgtcata
    29101 cacgttcaat ccgcttggta caagatttgg tgcactcaga gatgcatgaa tataatttgt
    29161 tgcaaaagta tttgcgttat aagtaatacc tgcgttcgag ttataaccaa atcctccgcc
    29221 gtctgtaaac aacgcaactt cattatcaaa gagattattt gccaatgcac caggtcctcc
    29281 gattattgta acgttgcctg taggtacatt aaagttatcg acacctgcac ctgcaagcag
    29341 tattccgcct gctccgtcag ctgccggacc attgtactga ttacctgaaa ttgtatttcc
    29401 tgtgattgtg ccataagaac cgtatccgaa ctgaattcca ttctgaccat ttacattctg
    29461 aatactttgt cctgttacta tattgtttgt tacaatagca tgcgtgttta attcattgat
    29521 cagaattcct gtcttctgat aatcttcaat cacattatca gaaacaatta ttgtatgatc
    29581 aaaattgaca tcgtatgtat ggttagcgaa gaatgcattt ccggactgga caccactata
    29641 aacagcatct cttactccgg tgattcttga atttgtaaaa ctaccgcctg cttcaaaata
    29701 atatatacca tagaattttg aaccgcctct gccatcaccg tcaattccga tatgactaat
    29761 gtcaacaact gttccggaat ctgagacata aacaactggt gtgtgatctg ctgcatttgc
    29821 attcggaaca agattcaaag acaatggagc tttaattact gcagcacttg cacctgcact
    29881 gtctgcacct gttattgtca aagatttatt gataataacc tgttcctgat aagtaccagc
    29941 atcaatgtaa attgtatcat tatcatttgc tactgaaaca gcatatgata ctgttaagaa
    30001 tggagctgaa gttgaacccg gatttgcatc acttccaact gcagttgtaa atacatcacc
    30061 tgcaagtgaa ttgtcgttaa caaaatatgc aatgatcggg aatccggtac atgaacccgg
    30121 tacaggctgg aatcctgctg taggtgcgtc attgtcagtt ccgtttacga gataaggaga
    30181 ataactcact gcaccaaaga cctttgttga gattacaaac tgatcagaag atccaaacca
    30241 gttacatgaa gcattttgtg tgacacctgt cagatcataa agtgcataag tatttgaact
    30301 cagatcattg tcatttacaa caccgtttaa agttgcacct gcatctttat agaatactac
    30361 accggcatcc cagccattca ctttattttc tgtagcagtt gcattaagtg tttctgttcc
    30421 taatgcatcc atttcaatac cggtaccatt tgttccgtca ctgatcagag tatttctgta
    30481 aatagatgta gtaatgatgt tcggattatc cggcagatta gaattctttc cagccagcag
    30541 atcttcttca aaaggagaag gcactactct tggatcgtca cccggatcag cgactatacc
    30601 ataataagtt gctgtaccat tgtttgctaa agtagaattg atcgtgtttt cattgatcat
    30661 tccgccgaca ttgatataat atataccaac ctgacatcct gtcagatcat tgtttgcggt
    30721 agttactgtt cctgttggat tagttaaata atataaaaga actccacagg aaacagttgt
    30781 cgatggtaca taagaaatat tactgataat attatttgta acagagccgg cagcgtcaaa
    30841 tccaatctgc actccatttt gcgcaataaa gcttaccggt cctgcacctg taattgtatt
    30901 actgtcaacg tttgcagtta gatcaattcc tgataatgaa gttgcatttt tctggaaatc
    30961 aaaaatatta ttactaataa tatccaatgt ccttgcattt ccatcatcag caagagcaaa
    31021 tattcctact ccagcctgtg caccatctat aggtgtattt cttattgctt tgatctcaca
    31081 atctcttact gtaccgcctg catttctgta accaataccc tggaatctgt tattggcatt
    31141 acctttgccg gcaccgtcaa ttgttacatc tttgatgatc acacctgctg catcatgaac
    31201 atatataaca ggtttgttta cacctgaagt aacataagat aatgtcagag tatttggaga
    31261 aataatattt gtaactcctg ttccctgacc gtctaatgta atatctttat ttacttcaag
    31321 ctgttctgta aatgatccag gacctacgtt cactacaaat ccaggggatg ctgcattgat
    31381 tcctctttgt acggaaggtg ttgtaattcc aggagctaca aaactattta cagttacgta
    31441 atcattatta ttttttacaa gaacgaatcc gatatttccg tcatcgatct tgtgctgaat
    31501 tctgtcttcg agttcaaaca gattaggaag tgtcataaca ttcggagact gaagacctgc
    31561 tccgacatta aagttattat ttcttccgtc aacatttaca tttacttttt ccttggtgga
    31621 tacataagta cctgtccata ttggatctcc tgatgtagct gtagtctgat tgttaagact
    31681 gatatttctc catacgtcag tattgaaatc attttcattt cctaatgtac cgacaattac
    31741 agttccgata gaagataaac tatcagaact tgcaagctct acagaaagac ctttctgatt
    31801 aaaagcagca ttaccattaa aagtattatt ggtaatggat gcattacttg tgaatgctgt
    31861 ctgaggagca gtggtacgct gtttcgaatc tattcttata gatttaaaaa gttctgctgt
    31921 cggatcaggt gtaaatgtat tgttatcaat atttacattt gaagaccttc ctgagaatac
    31981 tccgaagttc acataatttt caaaggtatt tccggtaact tctatgtttg cactgccgtt
    32041 tgaaatattt ttcaattcga gcattgcaaa gtcagtacct gaaccaaacg cattagatac
    32101 attggaaaca ttatttgcga aactatgtgt accgtttccg agagaattaa tttcagtttg
    32161 tccaaataat gaatcgttgg taatattcaa tataccacct tgtatatcgc ctgattgtat
    32221 ggcatagaat gctctgacta tactgttctt ccagtcgccg taacagttaa aggtcttgac
    32281 accgcgtcca agtggtgaat tacctgtatg cttaatgtca ttactgtcaa gattgacctg
    32341 gtcatactga acaccgccga atgttccaag ctgaattcca aaactgttga atattgctgc
    32401 accggttgta gctgtaccga atacgcaact gtttttaatt gtcagattat tgaaagttcc
    32461 tgaagctgca gctctgattc ctgtgttaac tgccagctga ttgaacttta ctattacatt
    32521 atcaatagta acattcggaa ctgttacaga aattgtctgg ctggctgagc cttctactac
    32581 cggacgtgat tcttttgtcg taccgctgcc ttttatagtg acttctttat ttatgactac
    32641 gttttcattg tagtatcctg aatcggctgt cagtacatgt ccgttcaatg tctgagcatc
    32701 atcaattgca gtctggatag aacagaacat ttcagttgaa ttcaaattca taactactcc
    32761 tgaacctgta ttagcaccga catctcgtgt atcaacagta ttagaaagaa tatttccgct
    32821 cattactata ccgcatgtct gaggtgaatt tcctcttccg aaatatgtat cagctacatt
    32881 attctgatct ccgtctccgg gatatggtaa atgacctgcc tgtctctgaa ttccaacatc
    32941 acataatgaa attgtatttc ctaatacgct gtgattcaga ccttcgacta cgattccaaa
    33001 tccatcactc gagctaatct gttgataacc gcttacagta ttattctgaa ctatggcacc
    33061 agttggcaca tcaacattac ccggcaatac gcctcttctg aatactgcaa tacctgcaag
    33121 gtctctga
//
LOCUS       DAORVQ010000013        32105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_440_length_32105_cov_5.314945, whole genome shotgun sequence.
ACCESSION   DAORVQ010000013 DAORVQ010000000
VERSION     DAORVQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 32105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32105
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_440_length_32105_cov_5.314945"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..385
                     /locus_tag="PLX80_02240"
     CDS             143..385
                     /locus_tag="PLX80_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19645.1"
                     /translation="MAKPIKANYDQEFLLPPSIEDWASKDHPARYIREFVQSLDLKEL
                     GFKEMTNEIDRPFYNSDLLLKIWLYGYFHRTRSSEK"
     gene            625..1794
                     /locus_tag="PLX80_02245"
     CDS             625..1794
                     /locus_tag="PLX80_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY19646.1"
                     /translation="MQKLLLGNSVQIVEINETIKQVAPTDVSVLITGESGSGKEVVAK
                     SIHLQSKRSDNPLITVNCGAIPEGIIESELFGHQKGAFTGAIDERKGYFEMADKGTIF
                     LDEIGEMPLSTQVKFLRVLETGEFMRVGGNKTISVDVRIVAATNKDLAYEMMNNQFRD
                     DLYYRLRSINIFIPPLRERRSDIKLLFYHFTELYCRENNIEFKGIDDDALDYLINFSW
                     PGNARELKNFCESIIVINPDKILTITDVKKHLQTDQKEMPSSLPILFGKSGNSGNNYN
                     KNNESPEKDFLFRALLEIKSDILDLKDQITDLSINKENKNGKDTGENDFKIPGDKLIN
                     MNMNDIEREVLVYLLNKNYWNVEKTAEILDQTPRNVYTKIKKYNISKEQSRGNYS"
     gene            1791..2306
                     /gene="lptE"
                     /locus_tag="PLX80_02250"
     CDS             1791..2306
                     /gene="lptE"
                     /locus_tag="PLX80_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQY19647.1"
                     /translation="MKMLKLFKIIFVLNAFLLMSGCGVYGFRGNNPPKGINTLAVPTA
                     EDISGFSAPTLPETFTERLKIRITTDNTFKIADKNISDGVLNCTITSVTDEASVIATG
                     DNVTQRKLTITIKVSFDDLKKQKQIWEKNFQNYGEYASNGNDFSNRESGVAIAIEKIA
                     EDIVIDLTSNW"
     assembly_gap    2502..2601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2698..4206
                     /locus_tag="PLX80_02255"
     CDS             2698..4206
                     /locus_tag="PLX80_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY19648.1"
                     /translation="MNIESLILAAYIISLSILFFFGSHGFNMVYYYFKTFKKRTVDLE
                     LSEFEMDDYPEVTIQIPLYNEQYVITRLIDSVLRMDYPKDKLEVQILDDSTDETTQII
                     TDHIGKYLEQGFDIKHIHRTNREGYKAGALKVGLETARGEFVAIFDADFIPRKKFLKR
                     TIPYFYKEEKLGLVQTRWEHLNREYSLMTKTQAIALDGHFVIEQAVRNRAGFFINFNG
                     TGGVWRKECIFDAGNWEADTLTEDLDLSYRAQMKGWKFRYLVNFTSPAELPSDIGALK
                     SQQFRWTKGAIETAKKVYPKVLRSDMSFKLKVQSFIHLYSNLAYPFILMAAILNLPVM
                     LIKLTGEYDTVFKFMSLFIFAFISSFIFYLYSQKDVYPDWQKRIIYFPVFLAGSMGFS
                     VNNTKAVFEGLLDKKSEFVRTPKYQIMNKKDSWEGKKYVNKKLAFTTYIEAVMAVYSF
                     TGVVIAIATAQVAAIPFQMMFFSGFTIISVLSIRQVIVSNRKVSQKLAMDAE"
     gene            4196..4963
                     /locus_tag="PLX80_02260"
     CDS             4196..4963
                     /locus_tag="PLX80_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19649.1"
                     /translation="MLNNFDKNIEESKAKLDKNYLSPLFIRLANLYYLNEQYDECISV
                     CRSGLSIYPDYLTIKLILVKALLKGEYLNEAEELFEELKYKIPVKEVVTKLRNKIQNL
                     RSISGQEKIHYPKDTKFKYDFSYFEKKFDPQENLFTEFTIDDLLNSDTEIRKESDIPE
                     FKYFRDKFLEFHLKLPADGKGKDIPNRNNKDSDSDIEKQIKIITETLADLYAEQKNYK
                     EAYEAYSFLLRAGSPNSKRIEEKLNDLERNMVKYDSI"
     gene            5163..6605
                     /locus_tag="PLX80_02265"
     CDS             5163..6605
                     /locus_tag="PLX80_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HQY19650.1"
                     /translation="MKLLQQKPIAWISGPLAALTVYWLPLPLETAAHQVAAIMIFCLV
                     FWITEVIPLSITALLGVTIAVMLGIADMQTAFTSLGHPIILLFFGSFLLSRAMIRHGL
                     DKRIALIVLSFPFFQKNLFRLFLGFSLVSFLLSMWVSNSVAVAMLLPLMIGVITLVQT
                     TDNPDKNLPVYMLLGIAYSASIGGISTIIGSPPNLIGVQYLSQFGIKVDFLKWMIMVL
                     PVSLSMYGFLLFYIYKKLKRCSFNEESISDYLKEHAGELRPFSTGERNTVIVFFFAVF
                     LWLLPGFLNLLGLESAYFFVIKILPDSLVALIAAILLYLLPCDKKLTGTLRAEDLRLI
                     DWNTILLFGGGLALGTLVINTGLAAFIGSSIADSVSEGNVPFIIFFLITGILFLTEIS
                     SNTAIAITFIPIVIDILTTLQLNVLYPVIGIVIASSFAFMMPISTPPNAIVFGSGLVP
                     VSKMLKTGLLLNITGALIIFFRVMVYIKFL"
     gene            complement(6755..8341)
                     /locus_tag="PLX80_02270"
     CDS             complement(6755..8341)
                     /locus_tag="PLX80_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HQY19651.1"
                     /translation="MNNFFTIKQTADFLNKNITGLEITEIFSQEKDKLCIVINSNSEE
                     KTLEFSADKKYPYLILRSNFSKARKNSVNLFQEIYGSKITGIDLNNSDRVILFTVSDG
                     HCLLFTFFTNKSNCFIFREQKILNAFKDREVCTGKDISDAVPEYNSGKLSPPETIADL
                     IKTSYRNFGKTYITEALHRTSLKKSTPADNESVNKIKEIFLTIGNELSSPVYYLYQKD
                     EDFILSLIKLSHLPDYNFKEYPDINELLTAFIKQSSFSKKFSDAKDNRLSELDKKISH
                     HVKKLDGLKVNLDHSIKSETLMQYGNLILQNIYRINRGDTVLSADPIEGTSDNIEIKL
                     KAELSPSENAEAYFDRYKKLKNSRSLLLNKISALEKEIIILQSEYKTIEASEDIKKLI
                     KENKKLTESKNDETNKFRKFVLNEKYQVWVGKDSISNDLLTTKYASPNDLWFHVRGAS
                     GSHTVLKISTKEPVPKEIITAAASISAYYSKARNAGNVPVAYCEKKFVKKKKGFKSGT
                     VTMEREKVIFVKPILPDGNN"
     gene            8746..10137
                     /gene="lpdA"
                     /locus_tag="PLX80_02275"
     CDS             8746..10137
                     /gene="lpdA"
                     /locus_tag="PLX80_02275"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQY19652.1"
                     /translation="MKEFDVIVIGTGPGGYTTAIRASQLGFKTAVIERDRLGGICLNW
                     GCIPTKALLKNAELMNTMNHLDDYGISVENVKFDFEKIIERSRGVADISEKGVRYLMK
                     KNKIEVFEGSAFINKDKTISVNDKEGKEIDKLKATYTIIATGARARGLGDLKFDEQRV
                     LSSTGAMILKDKPEKMTIVGSGAIGVEFAYFYNAFGTEVTIIEMMPNIMPIEDKDISD
                     VVAREFKKSGIKLLTGTKTESVEVKGNKVITKVSGKTNEDIESDVVLIAIGVVGNTEN
                     FGLNEIGIEVEKGSIKVDKDYKTNVDGFYAIGDVNGPPWLAHVASSEGINCIEKIKGL
                     HTPDIDYTTIPGCTYCQPQVASVGLTEMKALEEGYEINVGKYPFSASGKSRAMGETTG
                     MIKVIFDKKYDELLGAHIVGIEATELIMEFVLAKSLEATHEQILKTVHAHPTLSEANM
                     EAVGVAYNEAINI"
     gene            10246..10665
                     /locus_tag="PLX80_02280"
     CDS             10246..10665
                     /locus_tag="PLX80_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQY19653.1"
                     /translation="MDDNKTTKGLLLGFITGSVVGAAIALLYAPKSGREFRNDIRLKK
                     DEFWDDTTEYLQVAKSKATNLINEGKRKSEELISDARKKASSLIDDANEVLNDAKVKA
                     TDSYETTKDRVSSESGKIKDAFKAGIEAYNEEKKKSS"
     gene            10707..11144
                     /locus_tag="PLX80_02285"
     CDS             10707..11144
                     /locus_tag="PLX80_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19654.1"
                     /translation="MEETAFITQIIVNVIIIILFITIIVILLKVSKFLSSINKKIKSL
                     AEDSKEIKPKIIATLEKIGDLTERVSGISDKVTENIDVLGDVVEKIKDTTESVIEFEQ
                     KIQNAIEPPVMETVNTISAVTVGIKTFLDSWKNSRRNSNEYYD"
     gene            complement(11271..11699)
                     /locus_tag="PLX80_02290"
     CDS             complement(11271..11699)
                     /locus_tag="PLX80_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQY19655.1"
                     /translation="MIRNFLESKRDVGILILRLGTGISFLFIHGWNKFFGGPETWAKI
                     GKAMPTFGLEFLSVFWGFMAAISEFGGGLFLILGLFTRTTSSFMAFTMIVAFSKHLAN
                     LDPWSRVFYPVEMLSIFLALIFIGAGKYSLDALLFKKNSI"
     gene            complement(11806..12837)
                     /locus_tag="PLX80_02295"
     CDS             complement(11806..12837)
                     /locus_tag="PLX80_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQY19656.1"
                     /translation="MKAIVLNKTDRSENYSELFGIEERHVPEISGEEVLIKIKSAALN
                     HRDLFIAEGSYSKIKLPVIPGSDGAGIISKTAKNVTHFIEGDKVVIYPCNNWGESEEY
                     QNKNIEILGMPEDGTFSEFIKVHQSRVFKFPDHLNFSEAASFPLAGLTAYRALFEKAK
                     LKKNDNVLITGAGGGVSSMALIFAVSSGAVVFVTSGSNEKIEKAVSLGAAGGVNYRNE
                     NWHTELQSLSENKIDVIIDGTGGENYVKLIDIISCGGRIISYGATVGHAEKLDLHKVY
                     WKQLKIMGTTMGTVKDFLNMLEFIDIHNIKPVIDSIHSFSDYLNAFERMKNSEQFGKI
                     VIETDSSNK"
     gene            complement(12838..13659)
                     /locus_tag="PLX80_02300"
     CDS             complement(12838..13659)
                     /locus_tag="PLX80_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY19657.1"
                     /translation="MAKSKSNDTADPGIKSKNKIKIYSWNVNGIRAAQKKGFMEWMKK
                     SKGNIICLQETKAHSEQLDDTIKVVKGYSSHWFSAEKKGYSSVAIYSDTEPVNIIKGF
                     GNPDFDKEGRVIIAEYETFVLVNVYFPNGGRGPERIKYKLDFYDQLFFFLEKKYRDRK
                     GIIVVGDYNTAHMEIDLARPKENTTTSGFMPIEREWIDRLINLGYIDIFRKYNDKPQQ
                     YTYWDQITRARERNVGWRIDYFLVTPDIVDRISKAEIHSDVMGSDHCPISITLEI"
     gene            13763..16468
                     /locus_tag="PLX80_02305"
     CDS             13763..16468
                     /locus_tag="PLX80_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQY19658.1"
                     /translation="MNKSKSIKEIYNEIKDFDLVITNDVLLASALNRNVTSPRLGMLS
                     MTPRQLALKYGQIYFDRLYSKSELILEIYIHSKKSIRYIHQSVEKIFEIWNHTGLIES
                     CELYLGQEKELLKYFTEFKTVEFAMEKFDEKYYENKKIAIAGFKLFNELDKQVLPRKL
                     FPEFIKLMSEEKFTFQKTYLFNSITDLTSTISSLINKENQNEVALIFNTDSELKNIFT
                     SGLNQKGIKTEMKTFLKDDISVGKFLSFIESSFNISDHYVKDILFTESLFGINIDRKF
                     SNFNITGYLTTSDGLRTFQNIYDLMSNVEDHTFTELFDRIEFISGKKLIPELKNVLII
                     TELGEKKINSENFNVLKYFILNFDIETGKSEEGVLFVNASNAAFVDRQITFFIGMDDS
                     WTRLNMDKAYIEKKIEEEKDLEKFQILLSQGGEKVYLVLNIKKNQPVIPCYYFNMLSG
                     KDIKGFEDEFFNPVFAEIRPVKKEIRIAQGHNLKSGDSKYDGLISPSILNSFYTCPKN
                     FSYSKLLPQEEKSYFLKGTLLHDFAEFYFNHPEYCRKNFNNLLDKILKRYSGFLSEIN
                     KDKERTSFKIGMKVIMDFIESQNFDYEIEIPASGTWKKSGSKTDRSGIDFFKKLGMEK
                     KYTNTEKWMERFESCMRGKIDLTSGQTIVDYKSSRSGKSESALINEYKVERLAKDKAD
                     NANFQTIAYIAGKREDNPDEEINFIYLYLLLNSGDYIKGIQDPEKSITSVKYIPKTFK
                     RYIISDEFYELISTKHEVFTVLDHGMFSKLIKENFDDIDFYNIDSISGNLEDRIYSYL
                     NEDKGIDYTFFGNRKEETFRSKIIYPVFKEIFKARTGSKEYPHIYKDEVDEFLIFAKE
                     KIKEINECYKTDFPNRPVNDLRSVCMKCDYLNICIGNKLLTADEN"
     gene            16458..19442
                     /locus_tag="PLX80_02310"
     CDS             16458..19442
                     /locus_tag="PLX80_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQY19659.1"
                     /translation="MKTKITNINPQDTFADSLDGIYLVDAGAGTGKTHTIIRRYENLI
                     NKGVLPKDILLITFTRNAAEKMRNDVVTKKFGKDVNITDLLEAPVLNFHAFCLRFLKK
                     HGLNSPKLLGINEMLTGNFNVTENEVYENEVFRKFYLRFRKDNFKKYAGIYKSLGDDF
                     GSVHSIIKKLCSIGIFPTSEGWFGNGKELLNGNENRYNEKFDILNDVKIGEKKEKQND
                     MQNILKALKSNVFIDFNYEDWLDDKRVKEELKDIVFKDDEQELLIEFTRDVYFSYIEF
                     MLKKNILNFDFIIMFAFLILYNNKNVRETAQFEYIMVDEFQDTDELQFLLLMLLCKNI
                     DGHANIGVVGDWKQGIYGFRNTTIENITEFTERLSDYKKVLNSGEERISYNTYPEQIN
                     KITFEYNYRSSQKILDFSLSTLRCKGFEDDEVDINLLKENFNEALKARREFDDASEII
                     FYESAEEDSDSEAELVLMKISELTNDEKYIIRDFDNEGNTKSERRLKYSDICVLARTN
                     EFGLKLQKAGIDKNVPVNFDGGLELFASEQGILLLAWLRVMLNERDIAGWIPILEKDG
                     NKFNDLLYLKKLIEKGKINEEADYFKDLLSFREKLLSENKSLINIIEMIFTRYGFADV
                     YCNSIIAEFTNWTKSEILSPGELTNLIDSLSCSRFKVEINNTDDAVIYQTIHGAKGLE
                     YPVVILANLNSRIFPSFKNTINSIYYNPVTGLRSRKQFGTIGEYSGVFDNFRSEILIK
                     MMKTNNTDEERRLLYVAVTRAKQYLYFTARKPSLFFTELKKHTGMESVTDFKYEIIPV
                     KYESDISSEEIELEITKTDERKFVSVHSIMNEAGSEMESTGFFTDKENKEKYEEHSGI
                     DEDAKASIGLSLNEALTYGNKIHLAAQKLAENSDIKIENDELKRISDFLKSCGAAEIL
                     PEVEFLYPKGNDIIRGTIDLLLIYEDRIEIVDYKTDKSKKYIDKYKKQLSIYREVIRE
                     IYPDKKITAKIFYTGLNEVLEV"
     gene            19643..19960
                     /locus_tag="PLX80_02315"
     CDS             19643..19960
                     /locus_tag="PLX80_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQY19660.1"
                     /translation="MNDSKDTPNKSEEKRESTLKKVLTKPLFKADSKIAQYSGLGVSL
                     VVTILIFLWVGMWLDGKFETGFLFTLSLTFIGFAAGFYSFYLNIKKLTDADKKENPNY
                     NKF"
     gene            20373..21290
                     /gene="atpB"
                     /locus_tag="PLX80_02320"
     CDS             20373..21290
                     /gene="atpB"
                     /locus_tag="PLX80_02320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQY19661.1"
                     /translation="MHLTPSFKFSSILKRSSGLFLILFILITSLSQQTFADDHSGSDE
                     KFDIIEKVVDHDYVDFYFLGKLHLPKFEPVHFLGMTFDFSITKTLFMMFVSTFILILV
                     LLYAVSSNKKNKYPKGIGNLVESMVVFVRDDIVVPNMGKEGLKLMPFFLTLFFFIMFA
                     NLIGLFPFMAQPTKNINVTAGLAIITFIVTQIKGIQAHGLGGYAKALVPPGVPVFVLP
                     IMVVVEFIGLFTKPFSLLMRLFANITAGSIIIFSLIGLIFVMEYAGSAIAVPFALFIY
                     MMEIFIALLQAYIFTMLSVLYINMAMHSH"
     gene            21374..21595
                     /gene="atpE"
                     /locus_tag="PLX80_02325"
     CDS             21374..21595
                     /gene="atpE"
                     /locus_tag="PLX80_02325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQY19662.1"
                     /translation="MDLGFLGAGIGAGLTVIGAGIGIGRLAGSAMEASGRQPEALGEI
                     RTSMIIAAALVEGVALFGLVICILLATKS"
     gene            21669..22226
                     /gene="atpF"
                     /locus_tag="PLX80_02330"
     CDS             21669..22226
                     /gene="atpF"
                     /locus_tag="PLX80_02330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQY19663.1"
                     /translation="MIYYLSLLYNYSFIFLFNGDEKPMLLSINPGLIIWQLIIFALLL
                     ILLKKIAWKPLLSALNSREQSIKDAVESAENLKKDAEDMIAENKRVMAEANTQSMKII
                     NEAKEMSNKIGDEIKAKANEDSRKLIEQAKEEIKKEKESAMADLRNEVSDLAIKAAEK
                     ILNDNLDETRQKKIVNDFISQIPKN"
     gene            22254..22805
                     /gene="atpH"
                     /locus_tag="PLX80_02335"
     CDS             22254..22805
                     /gene="atpH"
                     /locus_tag="PLX80_02335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HQY19664.1"
                     /translation="MISKAARRYSLALYSIAEENGNTELIAKDIDNILNLINSNRELE
                     LFFVSPVISKSKKLTAVKDIFGNNSSELVMNFLRIIIERRREALVKDIFTDFLNLKKE
                     KQGIVDVMVKTSVELNEQEKENMKKSIEAYTKLKSDIKFGLDSSIIGGFVATINDTVL
                     DASIKRQLENLREKFRTGDHSLN"
     gene            22894..24414
                     /gene="atpA"
                     /locus_tag="PLX80_02340"
     CDS             22894..24414
                     /gene="atpA"
                     /locus_tag="PLX80_02340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQY19665.1"
                     /translation="MAEVKADEISAILRKQLSGFETEVDVYEVGTVLYVGDGVARVYG
                     LMKCEAGELIEFPNGVFGIALNLEEDNVGCILFGESSLVKEGDQVKRTKRIASMPVGE
                     AMLGRVINPLGQPVDGKGEIHTDKFLPLERKALGVIQRQPVKEPLQTGVKAVDAMIPI
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKDVFCIYVAIGQKGSTVAQVVKSLEDSGA
                     MEFTTVIVATASDPAPMQYIAAFAGATLGEYFRDSGRHALVVYDDLSKQAASYREVSL
                     LLRRPPGREAYPGDVFYLHSRLLERASKLSDDLGAGSLTALPIIETQEGDVSAYIPTN
                     VISITDGQIYLEPNLFNAGIRPAINVGISVSRVGGNAQIKAMKKIAGTLRLELAQFRE
                     LEAFAKFGSDLDKATQAQLRRGERLVEILKQKQYSPMPIENQVMVIYAATKGFMDEIP
                     ISHITRYQNDLLQEVENMHKGILDSIRDTKDLTKENTEKMEEVLKAFTERFKSTIN"
     gene            24430..25338
                     /gene="atpG"
                     /locus_tag="PLX80_02345"
     CDS             24430..25338
                     /gene="atpG"
                     /locus_tag="PLX80_02345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQY19666.1"
                     /translation="MATLKELKVRISGIKSTQKITKAMKMVASAKLRRAQERTIAARP
                     YAKKINELLTDLVSVAGEGTDELLESREIRSRCVVVVSSDRGLAGSFNTAVIRYAYNY
                     IKSLGDDTKVVVIGKKAYDALKKRDVNIVFHKTGIFSDLTIEVSNEIVNFLVDNYKKK
                     TFDSVEIIFNEFVTMVKQDTVKDQFLPLKKNKPNEKDGEKVKGKENIEVQEKKTAGDY
                     IYEPSESEIIKELIPKQLKVQMWKVLLESNTSEHAARMTAMETATKNATDLIMELELT
                     YNQARQAAITKEILEIVGGAEALKDA"
     gene            complement(25401..26852)
                     /locus_tag="PLX80_02350"
     CDS             complement(25401..26852)
                     /locus_tag="PLX80_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19667.1"
                     /translation="MHNSKLIQVLKTFSKKEIKQFREFTQSPFFNKNKSVISLLDVLI
                     KFYPEFQDKNLDAKNVFKIIFPDEEYDYFKIRNLSSDLLAVSMEFLKIIFFQKNEHQN
                     TLYLLEQLRDRDLDKMFQKLFDVYYQKIKSNHVRDEFYYSNMADLKNELKSFYSPKRP
                     NSDFNLIQEQLDYELTHFLIKALDLYTIMLHEIKQNNYEFKLEMFDEVLIYLEKNKDN
                     IAPVLKMFYLIIMLHKENDDRSFYELKDHGKKIFDSLGFYEKYMYFLHMTGFCAERYN
                     TDCSTDFMKEHFYLSKDNLETDTIVMGKIMYLDFVNHVKVAARVNEFDWAEKYITRFK
                     DQLNEEKESTLNFSYGYIEYMKGNRLKALELLSQTNFPNFIIKIQVKILILQISYEES
                     FYDQAIAMIDSFRHFLARENSVRENYKESFYDYMRITGELIRLKTSVKKKDLRSDLKR
                     LKDAAEKSTQNQFGIKLWLFKMIDNFDLKKTGI"
     gene            27169..30399
                     /locus_tag="PLX80_02355"
     CDS             27169..30399
                     /locus_tag="PLX80_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19668.1"
                     /translation="MKSIISLVIICALSVILTAGTAESQQNTTGNSKKVKTSSFHKKG
                     KKVTDQKTINEIISKNQKSYKKNLRGSWLLEEDFESGNFPPAGWSILNGATEWEQQFY
                     SGYGNGNYCMFYSSWQCNYSDNIIFTNDFNSNTQFGDKLIFDYAYAPYDDGNAYYDDL
                     EIYYYDAVEQIYYSLVYYYGWELQTASVGDQYFEPQSNEWGTMIVDIPENASSIYFKV
                     WENCANNLYIDNIKVGAVIGGYDASVEILWGKGKVPLSYGVPDKIPVLIKNLTSNPIN
                     NLMVSLEISGTNNLSDAIEIPYIGPLDTFQVDFLGFTPVLNGFSDITVSIPNDDDNGN
                     NEKTFITEANSNTIRYVDSNCCNSSVGWVGQNAFLNKYYMSGTGQIRTVNIKLPDWNF
                     GQIVYGIVLNSSGTIVGKSPHYKIKASDQNTYKSFQITDPKPFTVTNDYYYVGIAQTE
                     YAGDGFAYGSQQMLYELPARPDANYYSNLAPPGTNVGAYEFPREWGQNYAIEAVMGNQ
                     AATDAGISDLGLTYDQYFNSTTFTPVGKVFNAGTGSVSFSVRRTISPGGYTSTKSVTG
                     LAAGANAFVNYDPWTFTSGTVYTVKDSILISDGNNSNNVMTSNITPKIAKEMCILWQQ
                     QKDRDSLVRAVISDGRYANNFDTVGMYYTGSYRAWKVMIVNFKEEGSYSPWVRDSLKR
                     FIDNSTAGNKKSLIVFSDNTSLPNDPETGYMTPADTIFYRQYLKAKTISDDWIGSIPS
                     SQSKFRGVGFFNGISQDSVSDPYTPELIRPVNGSIAAFKPKSVTGNGSDSCNAVCFAG
                     ANYNTFFMTNQFSSLRATGGSPLSLGPVLVYTKIIDWLQSVNTNAKVLDLTALYEGLY
                     SIGQNTMAGDTVTVYLRNASSPYQKLDSSKGFLNSVGSESFIFNNASNGVNYYLHLKH
                     RNGLETWSKTPQQFSANYASYNFTVNANKAFGDNMILKGSKWTIYSGDVNQDGVIDID
                     DNSLIENAAFNFESGYIRTDLNGDEIADISDQAIADNNAYNFVSKSTPGGALIAPEIQ
                     EDYVPVNQSPDTEFKTDHEIYERTKNIQSNIKSNDLREKLFIKTNK"
     gene            30553..31911
                     /locus_tag="PLX80_02360"
     CDS             30553..31911
                     /locus_tag="PLX80_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HQY19669.1"
                     /translation="MLKKLPPSFISSDRLSLKIFIPVLFAVLYFGNTNVKAGDTTWVT
                     TFDQNFQNWADTHYGNFLLPDTNTHYKKITMYYTIGCPAAGCDPWDRIGWIKLYTDSV
                     ANVEIARIVTPYNIVGGGMPGQCAFEIDVTDYMSLLHDSVRLGSYIESWIGGTRGWLV
                     TVKFAFIEGEPEKIPYKVVNLWQDNRIVYGDTAVNPEAILVPREVAVDMGTSKAKVKI
                     ITTGHGQGNTDNAAEFSLKIHTLKVGAQNYDQVLWRSDCSVNPCSPQGGTWQYARAGW
                     CPGASVIPWDSDVTANITPGQKVIIDYDLYPYENFCRPTNPICISGQTCSDCNYNNNG
                     HTEPHYTIQGQLVLYKDNPAISVNNISTNTPENFMLHQNYPNPFNPSTKIKFDLPSFG
                     KVTLQIIDINGKTVDTLVEGKLNPGTYEAEWNAGGLSSGVYFYKLESGNFSQTNRMVL
                     IK"
BASE COUNT        10976 a         4939 c         6027 g        10063 t
ORIGIN      
        1 acttcataaa ctaaaacact ttgtcatttc cggttttact gcaggcattg tcgtaccgca
       61 gcctctgccg gctcctcctt aaaaaggaag tgtctcaaaa ctaattaggg aaatagttaa
      121 ttttgtgaaa caaaatttca agatggctaa accaataaaa gcaaattacg atcaggaatt
      181 tttacttcct ccatcaattg aagactgggc aagtaaagat catcctgcaa gatatatccg
      241 agagtttgta caaagtctgg atctgaaaga attgggattt aaagaaatga caaatgaaat
      301 tgacagacca ttttataatt ctgatctgct tttgaagata tggctttatg gatattttca
      361 caggacacga agttcagaaa aataaaaaga gaaacaaaaa gtaatatgcc attgatccgg
      421 cttagcggga tgaaatatcc tgatcataat acactttgga gattttggaa tcacaataaa
      481 tcaagcataa gcaaattatt taaaaaatct gtacagacag cagtaaagct tgatttagta
      541 tcaatggttg tacattaaaa attgaaaatt tataattgtc tttaataaac ctctccataa
      601 ccttatattt gtaaaatttt tttaatgcag aaattacttt tagggaattc cgttcagatc
      661 gtcgagatca acgaaaccat aaaacaggtt gcgccaacag atgtgagtgt actgatcacc
      721 ggtgaaagcg gttcaggtaa agaggtcgtt gcaaaatcca ttcatctgca gagtaaaaga
      781 agtgacaatc cgttgataac cgttaactgc ggtgctatcc ccgaaggcat tatcgaaagt
      841 gaattgttcg gtcatcagaa aggcgcgttc accggtgcca ttgatgaacg aaaaggttac
      901 ttcgaaatgg ctgacaaggg gacgatcttt cttgatgaaa tcggtgaaat gcctttgtcg
      961 acacaggtga aatttctgag agttctggag acaggcgagt ttatgagagt cggtggtaat
     1021 aagaccatct ctgtcgatgt aagaattgtc gcagctacga ataaagatct tgcatatgaa
     1081 atgatgaaca atcaattccg tgatgatctc tattaccgtc tgagatcgat aaacattttc
     1141 atacccccgc tgcgtgaaag aagatccgac attaaacttt tattttatca cttcacagag
     1201 ctgtactgca gagaaaataa tattgaattt aaagggattg atgatgatgc tctggattat
     1261 ctgataaatt tcagctggcc cggaaatgca cgtgagctga aaaatttttg tgaaagtatt
     1321 attgtaatta atcccgataa aatactgaca attacagatg ttaaaaaaca tcttcagact
     1381 gatcagaagg aaatgccaag ttcgctgcca atactttttg gaaagtcggg taattccgga
     1441 aataattaca ataagaataa tgaaagtccg gaaaaagatt ttttattcag agcattatta
     1501 gaaattaaat ctgacatatt agatctgaag gatcagatca ctgatctttc tattaataaa
     1561 gaaaacaaga atggaaaaga taccggagaa aatgatttta aaattccggg agataagctg
     1621 atcaatatga acatgaatga tattgaaagg gaagtccttg tatatctgct gaataagaat
     1681 tactggaacg tggaaaaaac agcggagata ctggatcaga caccgaggaa tgtctatact
     1741 aaaattaaaa aatataatat cagcaaagaa cagagcagag gaaactattc atgaagatgc
     1801 tgaagctttt taaaattata tttgtactga atgcattttt actgatgtcg ggatgcgggg
     1861 tttacggatt cagaggaaat aatcctccga aaggaattaa cacacttgca gttccgactg
     1921 ctgaagacat cagcggattc tcagcaccga cactgccgga gacatttact gaacggctga
     1981 agatcaggat cacaactgac aatactttca aaattgctga taagaatatt tcggacggtg
     2041 tgctgaattg tactattaca agcgtgactg acgaggcatc tgtaattgca acgggagaca
     2101 acgtgacaca aagaaaatta actatcacga tcaaggtttc ttttgatgat ctgaaaaaac
     2161 aaaagcagat atgggaaaag aattttcaga attacggtga gtatgcttcc aatggaaatg
     2221 atttttccaa cagggaatct ggagttgcaa ttgcaattga gaaaattgct gaggatattg
     2281 tgattgatct tacttctaac tggtaaattt ttgattttac ctctaagtct ctaaaactct
     2341 aaggctctaa aactctaaga ctcgaagact cgaaggctcg aaggagtttt ttaaaaaagt
     2401 tttttagctt atagtcttat agtcttagag gtaattccaa agttttcata caaggagaag
     2461 tatttatgga gaagtgtcaa aagtaacaaa aaatccgcac annnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn ngagccttag agttttagag tctttgaggt aattccaaag
     2641 ttttcagatg atgcgggaat tttggtaaaa tgataaattg gtaaactaaa tttaataatg
     2701 aatatagaaa gtctgatact tgctgcatac ataatttcac tctcgatact tttctttttc
     2761 ggatcgcatg gatttaatat ggtctattat tatttcaaga cctttaagaa aaggacagtg
     2821 gatttggagc tgagcgaatt tgaaatggat gattatccgg aagttacgat ccagattcct
     2881 ttgtataatg aacagtatgt gatcacacgg ctgatagatt ctgttttgag aatggattat
     2941 ccgaaagata aactcgaagt gcagatactt gatgactcta ccgacgagac tactcagatc
     3001 ataacagatc acatcggaaa atatctggag cagggatttg acataaagca tattcaccgc
     3061 acaaacaggg aaggatacaa agccggcgcg ctgaaggtcg gacttgaaac tgcccgcggg
     3121 gaatttgttg ccatattcga tgcagatttt attccgagga aaaaatttct gaagaggacc
     3181 ataccatatt tttataaaga ggaaaagctc ggactggtgc agacgagatg ggaacatctt
     3241 aacagggagt attcactcat gacaaagacg caggcgatcg cgctcgacgg acattttgtg
     3301 atagagcagg cagtgaggaa cagggcagga ttttttataa acttcaacgg cacaggcgga
     3361 gtgtggagaa aggaatgcat attcgatgcg ggcaactggg aagctgatac gctgacggaa
     3421 gatcttgatc tttcttacag agcgcagatg aagggatgga aattcagata tcttgtgaat
     3481 tttacgtcac ctgcagaact tccttctgac atcggagctc tgaagtcaca gcagttcaga
     3541 tggacaaaag gagctatcga aactgcaaag aaagtttatc caaaagtact gagatcagat
     3601 atgtctttca aacttaaagt tcagtcgttc atacatttgt acagcaatct tgcatatccc
     3661 tttatcttaa tggcagcaat tttaaatctg ccggtgatgc tgataaagct cacaggtgaa
     3721 tatgatacag tatttaaatt catgtcactt tttatattcg cgtttataag ttcattcata
     3781 ttttatcttt attcacaaaa ggatgtatat cctgactggc agaagcggat aatttatttt
     3841 cctgtatttc ttgcaggcag tatgggcttt tcggtgaata atacaaaagc ggtgtttgaa
     3901 ggattactgg ataagaaaag tgaatttgtc aggactccga aatatcagat aatgaacaaa
     3961 aaagattcat gggaaggtaa aaaatatgtg aataaaaaac tggcatttac aacttacata
     4021 gaagcggtaa tggcagtcta cagtttcacg ggagttgtca tcgcaatagc aacggcgcag
     4081 gttgcagcga tcccgtttca gatgatgttt ttcagcgggt tcacgatcat atcagtttta
     4141 tccataagac aggtaattgt atcaaacaga aaggtctcac aaaaattggc gatggatgct
     4201 gaataatttt gataaaaata ttgaagagtc aaaagcgaaa ttagataaga attatttgtc
     4261 tccgctgttc atccgacttg caaatctgta ttatctgaat gaacagtatg acgaatgcat
     4321 atcggtatgc agatccggac tcagcatata tcctgattac cttacaataa aattaatact
     4381 tgttaaagca ctgctcaaag gtgaatatct taacgaggca gaggaattat ttgaagaact
     4441 taaatataag atccctgtaa aggaagtcgt gacaaagctg agaaataaaa tccagaatct
     4501 gagatccatc tcgggtcagg agaaaattca ttatccaaag gatacaaaat tcaaatatga
     4561 ctttagttat tttgagaaaa aatttgatcc gcaggaaaat ctttttacag aatttacaat
     4621 tgacgatctt ttgaattctg atacagagat caggaaagaa tctgacattc ctgagtttaa
     4681 atatttcaga gataagtttt tagaatttca tctgaagctg ccggcagacg gtaaaggaaa
     4741 agatattcct aacaggaata ataaggattc tgattcagac attgaaaagc agataaagat
     4801 tattacagag acacttgctg atctttacgc cgagcaaaaa aattataagg aagcatacga
     4861 ggcatacagc tttctgctga gagcgggttc gcctaattca aaaaggattg aggaaaagct
     4921 gaatgacctt gagcggaata tggtgaagta tgacagtatt taatttactt tacttaaaga
     4981 agtacaggta atataaagat cacatttctt ttttcataat ttccttacag acgaatcaaa
     5041 aatattcagc aagttgttgc tcaaaatatt tcagaatgct ttttaattta agagcatact
     5101 atttcccgta atttctgtat tttaacttta cataaatagc tctttttgct ctgattcata
     5161 ctatgaagtt attacaacag aaacctattg cgtggatttc aggaccttta gctgcactta
     5221 ccgtttactg gttaccgctc cctttggaaa ctgcagccca tcaggttgcg gctattatga
     5281 tcttttgcct ggtattctgg attacagaag tgattcctct ttcaattacc gctttacttg
     5341 gcgttacgat tgcagtgatg ctcggtattg cagatatgca aaccgctttt acgagtttag
     5401 gtcatcccat aattttactt ttcttcggaa gttttttatt atctcgtgca atgatacggc
     5461 atggattaga taagcgtatt gcacttattg ttttatcttt tccgtttttt caaaagaatt
     5521 tattccgttt gtttctcgga ttttcattag tatcgttttt actttctatg tgggtgagca
     5581 attccgtagc agtggctatg ttactgcctc taatgatcgg agtaatcaca ttggtgcaaa
     5641 caacggacaa tccggataaa aatctgccgg tttatatgct tcttggtata gcttacagcg
     5701 catcaatcgg cggcatctca actatcatcg ggtcgcctcc aaatcttatt ggagtacagt
     5761 atttaagtca gtttggaata aaagttgatt ttttaaaatg gatgattatg gttttacctg
     5821 tttcattaag tatgtacggg ttcctgttgt tttatattta taagaaattg aaacgatgta
     5881 gttttaatga ggaaagtatt tctgattatc ttaaagaaca tgcaggtgaa ttgagaccct
     5941 tctctaccgg tgaacgcaac acggtaatcg ttttcttttt tgcagtattt ctttggttac
     6001 ttccgggatt cctgaattta cttggactgg aatcggcata tttttttgta atcaaaatcc
     6061 ttcctgacag cctggtagca ttgattgccg caatattact ttatttactg ccatgcgata
     6121 aaaagcttac gggtacactg cgtgcggaag atctccggct tatagactgg aatactattc
     6181 ttctattcgg aggcggttta gcattgggaa cacttgtgat caatacagga ctggcagctt
     6241 tcatcggcag cagtattgca gattctgtaa gcgaagggaa tgtacctttt ataatatttt
     6301 ttctgatcac aggaatttta tttttgacgg aaataagttc taatactgca attgcaatta
     6361 ccttcattcc tattgtaatt gatattctta ctacgcttca gttaaatgta ctttatccgg
     6421 ttattggaat tgtaattgct tcaagttttg cctttatgat gcctatatct accccgccta
     6481 atgcaatagt gtttggaagc ggtttggtac cggttagtaa aatgctgaag accggtttat
     6541 tgcttaacat tacaggagca ttgataattt ttttccgggt aatggtttat attaaatttt
     6601 tgtaaatctg tatttttcca tctgcaaagt cacagttctt atcttctgta atttctcctg
     6661 gtcaaaatgt ttacagagtt attaaattcg caaacttttt gaaacgtttc cagagactca
     6721 atattgctta ttcgtaattc gtaattcgta atttttaatt attcccatca ggaagtatag
     6781 gctttacaaa aataactttc tccctttcca tcgtcaccgt accggattta aatccttttt
     6841 tcttttttac aaatttcttt tcacagtaag cgacaggtac attgcctgca ttacgcgctt
     6901 tgctgtagta tgcggagatc gaagctgcgg ctgtgattat ctcttttgga accggttcct
     6961 ttgtgctgat cttcagaact gtatgtgaac cgctggcacc gcgcacatga aaccagagat
     7021 cattcggaga tgcatatttc gtggtcagaa ggtcattgga aatgctgtcc tttcccaccc
     7081 atacctgata cttttcattc aggacaaact tcctgaattt atttgtctcg tcgttttttg
     7141 attctgtcaa ctttttattt tctttgatta atttttttat gtcttcagat gcttcaattg
     7201 ttttgtattc actttgaaga atgattattt ctttttcgag agcagatatc ttattcagaa
     7261 ggagactgcg tgagtttttt aattttttgt atctgtcaaa atatgcttca gcattttccg
     7321 aaggtgaaag ttcagctttt aattttattt cgatattatc tgatgttcct tcaatcggat
     7381 ctgctgacaa taccgtgtca cctctgttta tccggtaaat attctgcaat atcagattcc
     7441 cgtactgcat cagcgtttca gattttatac tgtgatcaag attcactttc aatccgtcaa
     7501 gcttttttac atgatgactg atctttttat ccagctccga aagcctgtta tccttagcat
     7561 cggaaaattt ttttgaaaag gaagattgtt ttatgaatgc ggttagcagt tcatttatgt
     7621 caggatattc tttgaaatta taatcaggca agtgactaag tttaattaaa gataaaataa
     7681 agtcttcatc tttttgataa agataatata caggtgatga caattcattc ccgatggtta
     7741 gaaaaatttc tttaattttg ttaaccgatt cattatctgc cggagttgat ttttttaaag
     7801 aagttctgtg aagtgcttca gttatgtatg ttttgccaaa gtttcggtaa gaggttttta
     7861 tgaggtctgc gatggtttcg ggcggtgaga gtttgcctga attgtattca ggcacagcat
     7921 cggaaatatc tttgccggtg cagacttctc tgtctttgaa tgcattcagg attttctgct
     7981 cacgaaatat aaaacagttc gacttgtttg taaaaaaagt gaataacaga caatgtccat
     8041 ctgacacagt aaaaagaatt acccggtcag agttgttaag atcaattccc gttatctttg
     8101 agccgtaaat ttcctgaaaa agatttaccg aattcttccg ggctttggaa aaattacttc
     8161 ttaaaattaa gtaaggatat tttttatcag cggaaaattc aagagtcttt tcttctgagt
     8221 tactgtttat gacaatacat agtttatcct tttcctgtga aaaaatttct gtgatctcaa
     8281 gtcctgttat atttttattt agaaaatcgg ctgtctgctt tattgtaaaa aaattattca
     8341 atttatattt aattaaaatt ttctgttgtc ctgtacattt atatactctt tcatgatcgg
     8401 tgcaggatgt gtcacgctaa ggtaattttt ttttgaagta cttcttaact atgctgttgc
     8461 ttcaagatca ttctctaaaa gcctgttaat aatactgctg ccgaaacagc ctgtcccgcc
     8521 ggtgatgaat attccgggtt tttcatttta taaaaaatta aaacacagat ccttgatctg
     8581 tgctgctatg ccctccaaaa tagtcatatt aaaatttcag atatataata aaataccggt
     8641 aaattttaat tataaaaaat attacattga gatacggttt caatatttgt aatttacata
     8701 aacttacaaa aaacattcta aaatctaaat ctcttaataa tttacatgaa agaatttgat
     8761 gtcatagtga tcgggacagg tcccggaggt tatactactg ctattcgtgc ttcacaactt
     8821 ggttttaaaa ctgctgtcat agaaagagac agactcggcg gaatctgcct gaactgggga
     8881 tgcattccga ccaaagcact tctaaaaaat gctgaactta tgaatacaat gaatcacctt
     8941 gatgattatg gcatatctgt ggagaatgtg aaatttgatt tcgaaaaaat aattgagcgt
     9001 tccagaggtg tcgctgatat ttctgagaaa ggtgtaagat atctgatgaa aaaaaataag
     9061 atcgaagtat tcgaaggatc agcttttatt aataaggata aaacaatttc tgtaaatgat
     9121 aaagaaggaa aggaaattga caaattaaaa gcaacttata cgattatagc taccggtgcc
     9181 agagcgagag gattaggcga tctgaaattt gatgaacaaa gagttctttc atctacagga
     9241 gcaatgatat tgaaagataa gcctgaaaaa atgacgatag tcggcagcgg tgccattggt
     9301 gttgagtttg catattttta taatgcattc ggaactgaag tcactattat agaaatgatg
     9361 ccgaatatta tgccgataga agataaggat atttctgatg tagtcgccag agaatttaag
     9421 aaaagcggaa ttaaattact gacaggtact aagactgaaa gcgtggaagt aaaaggaaat
     9481 aaagtaatta caaaagtttc gggtaagacc aatgaggaca tagagtctga tgtagtactg
     9541 atagcaattg gtgtagttgg aaatacagaa aattttggtc ttaatgaaat tggaattgaa
     9601 gtcgaaaaag gaagtattaa agttgacaaa gattataaaa caaatgtcga tggattctat
     9661 gcgatcggtg atgtgaacgg tccaccgtgg cttgcacatg ttgcttcatc agagggtata
     9721 aactgtattg aaaaaattaa gggactgcat acacctgata tagattatac tacaattccc
     9781 ggctgtactt attgtcagcc ccaggtagca tctgtcgggc ttactgaaat gaaagctttg
     9841 gaggaagggt atgagatcaa tgtcggtaaa tatccttttt cagcgagtgg aaaatcgaga
     9901 gcgatgggtg aaactaccgg aatgataaaa gttatttttg ataaaaaata tgatgaactg
     9961 ctgggagcgc atatagtagg tattgaagcg actgaattaa taatggaatt tgtacttgca
    10021 aaatctcttg aagctactca cgagcagatt ctgaaaacgg ttcatgctca tccaacactc
    10081 agtgaagcaa atatggaagc agttggagta gcttataatg aagcaataaa tatctgatta
    10141 taataggtta tgataattag aaaattagat aatttgataa taaaagtaat ttattctata
    10201 tttatactaa aattaaattt aaattaaaac taaggagatt taaaaatgga cgacaataaa
    10261 acaacaaagg gactcttact tggatttata acaggtagtg tagtcggtgc agcaatcgct
    10321 ttactttatg ctccaaaaag cggtcgtgaa tttagaaatg atatcagatt aaagaaagat
    10381 gaattctggg atgatacaac tgaatatctt caggtggcaa aatccaaagc tacaaatctt
    10441 ataaatgagg gtaaaagaaa atccgaagag ctgatttctg atgcaagaaa aaaagcaagt
    10501 tcactgatcg atgatgcaaa tgaagtactt aatgatgcaa aagtaaaagc aacagattct
    10561 tacgaaacaa caaaagacag ggttagttct gaatcaggta agatcaaaga tgcttttaaa
    10621 gcaggcatcg aagcttacaa tgaagagaag aaaaaaagtt cgtaattatt atttaattta
    10681 tttttataaa aatttaaatc atataattgg aagagacagc ttttattacg cagatcatag
    10741 ttaatgtaat aattattata ctctttatta ctatcattgt gatattgtta aaggttagta
    10801 aatttttaag ttctataaac aagaaaataa aatccttagc agaagacagt aaagagatca
    10861 aaccaaaaat tattgcgaca ttagaaaaga tcggagatct tacagaaaga gtaagtggca
    10921 tatctgataa ggttactgaa aatatcgatg tgcttggaga tgtagtagaa aaaataaaag
    10981 atactacaga aagtgtgatt gagtttgaac agaaaattca gaatgctata gagccgccgg
    11041 taatggaaac tgtcaatact atcagcgcag ttacggttgg tataaagact ttcttagatt
    11101 catggaaaaa cagcagaaga aattccaatg agtattatga ttaaggaaga ttaatttttc
    11161 tgataaaaaa tcttttgatc tgaaaaacag agtaacggtt agaatataaa aatccagacg
    11221 atccggttta aggatcgtct gatttttttt aaaaaatttt agaatagctg ttaaatacta
    11281 ttttttttga aaagcaaagc atcaagacta tatttgccgg ctccgataaa gatcaatgca
    11341 agaaatatcg aaagcatttc aaccggatag aaaacccttg accatggatc aagatttgca
    11401 agatgttttg aaaaagctac gatcatagta aatgccataa aggaggaagt agtccttgta
    11461 aataaaccta agatcagaaa taaacctccg ccgaattcac ttatagctgc cataaatccc
    11521 caaaatacag aaagaaattc taatccgaat gttggcattg ctttaccgat ctttgcccat
    11581 gtctccggtc cgccaaaaaa cttattccat ccatgtataa ataaaaatga aattcctgtc
    11641 ccaagcctga gaattaaaat accaacatct ctttttgatt ctaaaaaatt cctgatcatg
    11701 atttatattt tgatttgtaa aatggtttga aaatactttt attttaatga atgttgaata
    11761 gtttaagttc ctttaattta tttcaattgt tttaatcaga tcagattatt tattgctgct
    11821 gtcagtctca attacaattt tcccaaactg ctcagagttt ttcatccttt caaaagcatt
    11881 cagataatct gagaaactat gaatgctgtc aattacaggt ttgatgttat gaatgtcaat
    11941 aaattccagc atatttaaaa agtctttgac ggtacccata gtcgttccca ttatttttaa
    12001 ctgtttccag taaactttat gaaggtcaag cttttcggcg tgacctactg tagctccgta
    12061 tgaaattatc cttccaccgc agctgatgat atcaataagt ttaacatagt tttctccgcc
    12121 tgttccatca atgatcacat cgatcttatt ttccgaaagt gactgcagtt ccgtatgcca
    12181 gttctcattt ctgtaattca ctccgcctgc tgcgccgagt gaaactgctt tctcaatttt
    12241 ttcattactg cctgaagtaa caaatactac ggctccggat gaaactgcaa atattaatgc
    12301 cattgaagaa acccccccgc ctgcgccggt aattaaaaca ttatcattct tttttaactt
    12361 agctttttcg aataatgccc tgtaagcagt tagtcctgcc aatggaaatg aagcagcttc
    12421 agaaaaattt aaatgatcag gaaatttaaa aactcttgat tgatgaactt taataaattc
    12481 cgaaaaagta ccatcttcag gcattccgag aatttcaata tttttatttt gatattcttc
    12541 tgattctccc cagttattgc agggatagat cacaacttta tcaccttcaa taaaatgggt
    12601 aacattcttt gctgtcttag aaatgatacc tgcaccatct gacccaggaa taacaggaag
    12661 tttgatcttt gaatatgaac cttccgcaat aaatagatca cgatgattca gagcagcact
    12721 tttaattttt atcaaaactt cctcacctga aatttcgggc acatgtcttt cttcaattcc
    12781 aaaaagttcc gaataatttt cagatctgtc tgttttattt aaaactatcg ctttcattta
    12841 tatttccagt gtgattgata tcggacaatg atcgctgccc atgacatcag aatgaatttc
    12901 agcttttgaa attctgtcaa caatatccgg tgtaaccaaa aaataatcta ttctccaacc
    12961 tacattcctt tcacgtgctc ttgtgatctg atcccagtat gtatattgct gcggcttatc
    13021 attgtatttt ctgaaaatat cgatgtagcc gagattgatc agacggtcta tccattctct
    13081 ttcgatgggc ataaatcctg aagtagttgt attctctttt ggacgggcca ggtcaatttc
    13141 catgtgggca gtattgtaat cgccgacaac tataattcct tttctgtcac ggtatttttt
    13201 ttcaagaaag aaaaaaagct gatcataaaa atcgagcttg tattttattc tttccggacc
    13261 tctgcctccg tttgggaaat atacatttac aagtacaaag gtttcgtatt cagcaattat
    13321 cactcttcct tctttgtcaa aatcaggatt tccaaaaccc ttaataatat ttaccggctc
    13381 agtatcacta taaatggcta cactggagta accttttttt tctgctgaaa accaatgtga
    13441 agaatatcct ttaactactt taattgtatc atcaagttgt tcggagtgtg ctttcgtttc
    13501 ctgaagacaa atgatattac ctttactttt tttcatccat tccataaatc cttttttctg
    13561 agcggctctg atgccgttca cattccagga atagatctta attttattct tactttttat
    13621 accgggatca gctgtgtcat tagatttact ttttgccatt ttaaatattt ataagtttga
    13681 agtaacatag aatttttttt atgaagttaa ttaactattt tgaaaattaa tattcaattt
    13741 atccaattct ataattgatc tgatgaacaa atccaaaagt attaaagaaa tttacaatga
    13801 aattaaggac tttgatcttg taataaccaa tgatgttctg cttgcttctg ctctcaatag
    13861 aaatgtcact tcaccacggc tcggaatgct ttcaatgact ccaagacagc ttgctcttaa
    13921 atatggtcag atatattttg acagactgta ttctaagtcc gaacttatac ttgaaattta
    13981 catacattca aaaaaatcaa tcagatatat tcatcagagt gtcgaaaaga tttttgaaat
    14041 atggaatcat accgggctca tcgaaagctg tgaattatat ctgggacagg aaaaagaact
    14101 tcttaaatat ttcactgaat tcaaaacagt tgaatttgct atggagaaat ttgatgagaa
    14161 atattatgag aataagaaaa tagcaattgc aggttttaaa ctttttaatg aattagataa
    14221 acaggtactt ccgagaaaac tttttccgga gtttattaaa ctcatgtctg aagaaaaatt
    14281 cacattccaa aaaacttatc tgttcaattc aattactgat ttgacaagta ccatctcttc
    14341 attaattaat aaagagaatc aaaatgaagt cgctttgata tttaatactg acagtgaatt
    14401 aaaaaatata ttcacatcag gattaaatca aaaaggtatt aagacagaaa tgaaaacttt
    14461 cttaaaagat gatatttcag ttgggaaatt tttatctttt attgagtcat ctttcaatat
    14521 ttcagatcat tatgtaaaag atattctttt taccgaatct cttttcggaa taaatattga
    14581 cagaaagttt tcgaatttta atataacagg ttatttaacc acgagcgacg gtttgagaac
    14641 atttcaaaat atatatgacc taatgtctaa tgttgaagat catactttta cggaactctt
    14701 tgacaggatc gaatttatat caggtaaaaa attaattcct gaattgaaaa atgttttgat
    14761 aataacagaa cttggagaaa agaaaataaa ttctgaaaat tttaatgtac tgaaatattt
    14821 tattttgaat tttgatattg aaacaggtaa atcagaagaa ggcgtactgt ttgtaaatgc
    14881 atcaaacgct gcattcgttg acagacagat cacatttttt ataggaatgg atgacagttg
    14941 gacaagattg aatatggata aagcttatat tgaaaaaaaa attgaagaag aaaaagatct
    15001 tgaaaaattc cagatacttc tttcgcaggg aggggaaaaa gtttatctgg tattaaacat
    15061 taaaaagaat cagccggtaa taccgtgtta ttattttaat atgctttcag gaaaagatat
    15121 aaagggattt gaagatgaat tttttaatcc tgtatttgct gagataagac cggttaaaaa
    15181 ggaaattaga attgcacagg gacataattt gaaatcaggg gattcaaaat atgatggatt
    15241 aatttctcct tctatactga attcctttta tacctgtccg aaaaattttt catacagcaa
    15301 gcttttaccg caggaggaaa aaagttattt cctgaaagga acacttctcc atgattttgc
    15361 tgaattttac tttaatcatc ctgaatattg caggaaaaac tttaacaatt tgttagataa
    15421 aattttgaaa agatattcgg gttttttatc ggaaataaat aaagataaag aaaggacatc
    15481 ttttaagatt ggtatgaaag taattatgga tttcattgag agtcagaatt ttgattatga
    15541 aattgaaata ccggcatcag gtacctggaa aaaatccggc agtaagacag acagatcagg
    15601 cattgatttt tttaaaaagc tgggaatgga aaaaaaatat acgaatacag agaaatggat
    15661 ggaaagattt gaatcatgta tgaggggaaa gatagatctt acatccggtc aaactatagt
    15721 agattacaaa tcaagcagat ccggtaaatc cgaaagtgct ttgataaatg aatataaagt
    15781 agaacggctt gcgaaagata aagctgacaa tgctaacttt cagactatag cttatattgc
    15841 aggtaagcgg gaagacaacc ctgatgaaga aattaatttt atatatcttt atttacttct
    15901 taattcagga gattacatta agggtattca ggatcctgaa aaatcaatta cgtctgtcaa
    15961 atatatccct aagactttca aaagatatat aatctctgat gagttttatg agttgataag
    16021 taccaagcat gaagtgttca ccgtgcttga tcatggaatg tttagtaaac tgataaaaga
    16081 aaatttcgat gacatagatt tttataatat tgattcaatt tcaggaaatc ttgaagacag
    16141 gatctactcg tatttgaatg aagacaaagg aattgattat acattttttg gtaacagaaa
    16201 agaggagaca ttcagaagta aaattatata tcctgtattt aaagaaatat tcaaagcccg
    16261 cacaggctct aaagaatatc cgcatattta taaagatgaa gttgatgaat tcctcatatt
    16321 tgccaaagag aaaattaaag agatcaatga atgttacaaa actgattttc caaacagacc
    16381 tgtaaatgat ctgcgaagtg tatgtatgaa atgtgattat cttaatatct gtattggaaa
    16441 taaattactt actgctgatg aaaactaaaa ttacaaatat aaatccacag gatacttttg
    16501 ctgattcact ggatggaatt tatctggttg atgcaggagc cggaacagga aagacacata
    16561 ctattatccg acgttatgaa aatcttatca ataaaggtgt gctgccgaaa gacattctgc
    16621 tgatcacatt caccaggaat gcagcagaga aaatgagaaa tgatgtagtc acgaaaaaat
    16681 tcgggaaaga tgtaaacatc acagacctat tggaagcacc tgttttgaat ttccatgcat
    16741 tttgtctgag atttcttaag aagcacggac tcaattcacc aaagctgctg ggaataaatg
    16801 aaatgctgac aggtaatttt aatgtaactg aaaatgaagt ttatgaaaat gaggtcttca
    16861 gaaaatttta tctcagattt agaaaagata atttcaaaaa atatgcaggt atatataaaa
    16921 gtctcggaga tgatttcggt tcagtacatt ctataataaa aaagctttgt agtataggga
    16981 tatttccgac atctgaaggt tggtttggaa atggaaaaga attgttaaac gggaatgaaa
    17041 atagatataa tgaaaaattt gacattctta atgatgttaa aataggtgag aaaaaggaga
    17101 agcagaatga tatgcagaat attctaaaag cattgaaatc aaatgtattt attgatttta
    17161 attatgaaga ttggctggat gataagagag ttaaagaaga attaaaggat attgtattta
    17221 aggatgatga gcaggagttg ctgatcgaat ttacccgtga cgtttatttt tcatatatag
    17281 aatttatgct gaagaaaaat attctgaatt ttgattttat tataatgttt gcttttctaa
    17341 ttctttataa taataaaaat gtcagagaga ctgcacagtt tgaatatata atggtggatg
    17401 aatttcagga cacagacgaa ttgcagttcc tgttgcttat gcttttatgt aaaaatattg
    17461 acgggcatgc aaatataggt gtagtcggcg actggaaaca ggggatctat ggattcagaa
    17521 atacaactat agaaaacatc actgaattta cagaaagact atctgattat aaaaaagttt
    17581 taaactcagg tgaagaaaga atttcctata acacttatcc tgaacaaata aataaaatta
    17641 cttttgaata taattacagg agttctcaga agatattgga ctttagtctg agtactctcc
    17701 gctgtaaagg ttttgaagat gatgaagttg atataaactt acttaaagaa aatttcaatg
    17761 aagcactgaa ggcaagaaga gaatttgatg atgcttcaga gataatattt tatgagtctg
    17821 ctgaggaaga ttcggatagt gaggcagagc ttgtacttat gaaaatctca gagctgacaa
    17881 atgatgagaa atatattatc agggattttg ataatgaagg aaatacaaaa agtgaaagga
    17941 gattaaaata ttctgacatt tgtgttttgg caaggacaaa tgaattcgga ctgaaacttc
    18001 agaaagcggg tattgataaa aacgttccag ttaactttga cggtggcctt gaactgtttg
    18061 catctgagca gggaattctt ttgcttgcct ggctgagagt aatgcttaat gaaagagata
    18121 ttgcaggctg gatacctata cttgaaaagg acggaaataa atttaatgat ttactttatc
    18181 tgaaaaaact tatagaaaaa ggaaagatca atgaggaagc tgattatttt aaagatctgc
    18241 tatctttcag ggaaaaatta ttgtcagaaa ataaaagtct tatcaatata attgaaatga
    18301 tctttacccg ttatggattt gctgatgttt attgtaattc gattattgca gagttcacaa
    18361 actggactaa gagtgaaatt ctttcacccg gtgaacttac aaatttaata gattcattat
    18421 cgtgcagcag atttaaagtt gaaataaata atactgatga tgctgtaatt tatcagacta
    18481 ttcacggagc taagggactg gaatatcctg ttgtgatact ggctaacctg aactcaagaa
    18541 tattcccatc atttaaaaat acgataaata gtatttatta taatcctgta acgggactca
    18601 gatcaagaaa acaattcgga acgataggtg aatattctgg agtgtttgat aatttcagat
    18661 cagagatcct gataaagatg atgaagacaa ataatactga tgaagaaaga aggcttcttt
    18721 atgtggctgt gacacgggca aaacagtatt tgtattttac tgcgagaaag ccgtcattat
    18781 tttttactga actcaaaaag cataccggaa tggaatcggt aactgatttt aaatatgaaa
    18841 taatacctgt aaaatatgag tcagatattt cgagcgaaga aattgaactt gagatcacaa
    18901 aaactgatga aagaaagttt gtgtctgttc atagtattat gaatgaagcg ggaagtgaaa
    18961 tggaaagtac aggatttttt actgacaaag aaaataaaga aaagtatgag gaacattcgg
    19021 gcatagatga agatgcaaaa gcatcaattg gactgagttt aaatgaagca cttacttatg
    19081 gaaataagat ccatcttgca gcacagaaac ttgctgaaaa ttctgatatt aaaattgaga
    19141 atgatgagct taaaaggatt tcagattttc tgaaaagctg cggagctgct gagatattac
    19201 ctgaggtaga gtttctttat ccgaaaggaa atgacattat aagaggaacg attgatctgt
    19261 tgctgatcta tgaagacaga attgagatcg tagattataa aacagataag agtaaaaaat
    19321 atattgataa atataaaaag cagctgagta tttacagaga agtgatcagg gaaatatatc
    19381 ctgacaaaaa aataacagca aaaattttct ataccggtct gaatgaagta ttggaagttt
    19441 gaaaataatt caaagatagt ctgactattg tgaaataatc ccgtttgaaa atatatatct
    19501 gttattttaa ttttttaaaa attaatacta aataaagtat taagcgattt aagaaaaaaa
    19561 ttaatttttt tcgaaatccc gataaagctg aattgatttt acaacacatt ttgattaaat
    19621 ttataatcgc taataatgtt ttatgaatga ctcaaaagac actccgaaca agagtgaaga
    19681 aaagagagaa agtactttaa agaaggtact taccaaacca ttatttaaag cagattcaaa
    19741 aattgctcag tattcaggat taggtgtttc attggttgtt acgatcctga tttttttatg
    19801 ggtgggtatg tggcttgacg gtaaatttga aactgggttt ttgttcacat tatctttaac
    19861 tttcatagga tttgcagcag ggttttacag tttttatctt aacattaaaa agctgactga
    19921 tgcagacaag aaagaaaatc caaactacaa taagttctga atacattaga ttttaaaaag
    19981 ttcattctca aaattgttat aataagtgtt gttcttttaa cagttgcatt cgttttaatt
    20041 tcagtcagag atcttgaggt ttatacatta cctttaatca tcggatggtt aattagtttt
    20101 gcaaatgttg taattgggag ctatataatt acaaaagctt tcagagacca gggaaaaggg
    20161 tttttcaaca cagttctttt aagtatggta gtaaggatgt tttcgatcgc agcattggtc
    20221 ttcgtcctga tctacttttt gaaattagat aagattggat ttagtgtaat tttatttttc
    20281 ttttattttt tattcatgat actggaaata aattacatgt catcctccaa aaaaaataca
    20341 atcaatcaag tatcataaaa atttttaata ttttgcatct gacaccctcg tttaaattca
    20401 gttcaatcct aaaaagatca tccggactat ttttaatttt attcatatta attacgtctt
    20461 tatcacagca gacttttgct gatgatcatt caggaagtga tgaaaaattt gacattattg
    20521 aaaaagtagt cgatcatgac tatgtagact tttatttcct tggaaagctg cacctgccaa
    20581 agtttgagcc ggtacatttt cttggaatga cttttgattt ctcgatcaca aagactttgt
    20641 tcatgatgtt cgtatcgact ttcattctta tccttgtgct tttatatgca gtgtccagta
    20701 ataagaaaaa taaatatccg aaagggatcg gaaatcttgt tgagtcaatg gtagtatttg
    20761 tcagagatga cattgtcgta ccaaacatgg gaaaagaagg attaaaactg atgccgtttt
    20821 ttctcacatt atttttcttt ataatgtttg caaatcttat cggactgttt ccttttatgg
    20881 ctcagccgac aaagaatatt aacgtaacag ccggtcttgc aattattaca tttatagtta
    20941 ctcagatcaa aggaattcag gcacacggat taggcggata tgccaaagca cttgtaccac
    21001 ccggagttcc ggtgtttgta cttccaatta tggtagtcgt agaattcatt ggattattta
    21061 caaagccatt ttcattactg atgaggcttt ttgcaaatat cacagcggga tcgataatta
    21121 tattttcact gataggactt atttttgtaa tggaatatgc aggctcagca atcgcagttc
    21181 cattcgcttt atttatttac atgatggaaa tttttattgc actgctgcag gcatatattt
    21241 tcacaatgct ttcggtttta tatattaaca tggcaatgca ttcgcattaa catgttttaa
    21301 attttaaaaa aaagagaaac aaaataaaaa taaattaaaa taacttaaac acaacaaagg
    21361 agactaatct aaaatggatt taggattttt aggagccggt ataggtgccg gattaacagt
    21421 aattggagca ggtatcggaa tcggaagact tgcaggatca gcaatggaag caagtggaag
    21481 acagcctgaa gctttaggcg agatcagaac atcaatgatc attgcagcag cgcttgtaga
    21541 aggagttgct ctattcggac ttgttatctg tattttgctt gctacaaaaa gctaatcagt
    21601 aaaagctagt attccaaaat cggttaatat tatattaaac cgttatataa atttaaataa
    21661 atcagataat gatatattat ctttcattac tttacaatta ttcctttata tttttattca
    21721 acggggatga aaagccgatg cttttatcca tcaatccggg actgataata tggcagctca
    21781 ttatatttgc gttgctgctg attcttttga aaaagatcgc atggaagcca ttgctttcag
    21841 ctttgaattc aagagagcag tctattaagg atgcagtcga gtcggctgaa aatcttaaga
    21901 aagacgctga agatatgatc gcagaaaata aaagagtcat ggctgaagca aatactcagt
    21961 ctatgaagat cattaatgaa gcgaaagaaa tgtcgaataa aataggggat gagatcaaag
    22021 ccaaagccaa tgaggattca agaaaactta ttgaacaggc taaagaagaa ataaagaaag
    22081 agaaagaatc tgcaatggca gacctgagaa acgaagtttc tgatcttgcc ataaaagcgg
    22141 ctgagaaaat attaaatgac aatcttgatg aaaccaggca aaagaagata gtcaatgatt
    22201 ttatttctca gataccaaaa aattaatact gtattactaa agaataatca gacatgatct
    22261 caaaagcagc aagaagatat tcattagctt tatattcgat agcagaagag aacggtaaca
    22321 ctgaacttat tgctaaggat atagacaata ttcttaactt aattaattca aacagagaac
    22381 ttgaactgtt ctttgttagt cctgtcatca gcaagagcaa aaagcttact gctgtaaagg
    22441 atattttcgg taacaactct tcagagctgg ttatgaattt tctaagaatt attattgaaa
    22501 gaagaagaga ggcacttgtc aaagatatct ttactgattt tctgaatctc aaaaaagaaa
    22561 agcagggaat agttgatgtg atggttaaaa cttctgtaga actgaatgaa caggaaaaag
    22621 agaatatgaa aaaaagtata gaagcttata cgaaattaaa gagtgatatt aaattcggat
    22681 tagacagttc cattattggc ggctttgttg ctacgataaa tgatactgta ctggatgcaa
    22741 gcattaaaag acagcttgag aatctcagag aaaaattcag aaccggtgat cattcactta
    22801 attagtttta tcagttataa aggttttaga aattcaaaca aaattttaac aattaataaa
    22861 tattaacatt tttaatatta ggagatataa acaatggcag aggtaaaagc agacgagatt
    22921 tccgcgatac tcagaaagca gttgtcggga tttgaaacgg aagtcgatgt atatgaagtg
    22981 ggtactgttt tatacgttgg agacggtgta gcaagggttt atggcttaat gaaatgtgaa
    23041 gccggtgaac ttatagaatt tccaaacggt gtattcggaa tagctcttaa ccttgaagaa
    23101 gacaacgtag gttgtattct atttggagaa tcttcattag taaaagaagg tgatcaggta
    23161 aagagaacta aaaggatcgc atcgatgccc gtaggtgaag cgatgctagg aagagtaatc
    23221 aatcctctcg gacagccggt tgacggaaaa ggtgaaattc atactgataa atttcttcct
    23281 ctggaaagaa aagctcttgg tgtaattcaa agacagccgg taaaagagcc gctgcagaca
    23341 ggtgtaaaag cagtagatgc tatgattccg atcggcagag gtcagcgtga acttatcatc
    23401 ggtgacagac agaccggtaa gactgcagtt gctgttgata ctattatcaa tcagaaaggc
    23461 aaagatgtat tttgtatata tgtggctatc ggacagaaag gttcgacagt tgctcaggta
    23521 gtaaaaagtc ttgaagattc aggagctatg gaatttacaa cagttattgt agctactgct
    23581 tctgatcctg cacctatgca gtatattgct gcttttgcgg gagcaactct tggtgaatat
    23641 ttcagagatt caggaagaca tgcattagta gtttatgatg acctttcaaa acaggcagca
    23701 tcttacagag aagtttcact tctattaaga agacctccgg gtcgtgaagc atatccggga
    23761 gacgttttct atctccactc aagattactt gagagagctt caaaactttc tgatgatctt
    23821 ggtgcgggaa gtctaacggc tttacctatt attgaaactc aggaaggtga cgtgtctgca
    23881 tatattccga ccaacgtaat ttctattact gacggtcaga tatatctcga accaaatcta
    23941 tttaacgccg gtatcagacc tgctatcaac gtaggtattt ccgtatcgag agttggtggt
    24001 aatgcacaga tcaaagcaat gaaaaaaatt gcaggtacac tcagacttga gcttgcacag
    24061 ttcagagagc tggaagcttt tgctaaattt ggttcagatc ttgataaagc tacacaggca
    24121 cagcttagaa gaggagaaag acttgtagag atacttaagc agaaacagta ttctccaatg
    24181 cctattgaaa atcaggtgat ggttatttat gctgcaacta aaggctttat ggatgagata
    24241 cctatttcac atattacaag atatcagaat gatcttttac aggaagtaga aaatatgcat
    24301 aagggaattc tcgacagtat cagagataca aaagatctga caaaagaaaa tactgaaaag
    24361 atggaagaag ttttaaaagc atttacagaa agatttaagt caactattaa ttaataaagc
    24421 ggacaggaat tggcaacatt aaaagaatta aaagttcgga tcagcggtat caagagtact
    24481 cagaagatca ccaaagcaat gaagatggtg gcatctgcaa aactcagaag agctcaggaa
    24541 agaaccatag cagcaagacc ttatgcaaaa aagatcaatg aacttcttac tgatcttgta
    24601 agtgtagcag gcgaaggtac tgatgaactc cttgaatcac gcgagatcag atccagatgc
    24661 gttgtagtag tttcatctga cagaggactt gcgggttcat tcaatactgc tgtaatcaga
    24721 tacgcatata attatatcaa aagtctcggt gacgatacta aggttgttgt aatcggaaaa
    24781 aaagcttatg atgcattaaa gaaaagagat gtaaatattg tattccataa aacaggaata
    24841 ttcagtgatc tgactattga agtttcaaat gagattgtga attttcttgt ggataattat
    24901 aagaagaaga cttttgattc ggtggaaatt attttcaatg aatttgtaac gatggtaaaa
    24961 caggatactg tgaaagatca gttcctgcct ttgaaaaaga ataagccaaa tgaaaaagat
    25021 ggtgagaaag taaaaggaaa agaaaatatt gaagttcagg aaaagaagac agcaggtgat
    25081 tatatttatg aaccttctga atcagagatt attaaagagt taattcctaa acaattgaaa
    25141 gtgcagatgt ggaaagttct gcttgaatca aacacttcag agcatgcagc cagaatgact
    25201 gcaatggaaa ctgctaccaa gaatgcaact gatcttataa tggaactgga acttacatac
    25261 aatcaggcaa gacaggcagc gatcacaaaa gaaatacttg agatcgtcgg tggtgcagaa
    25321 gcactcaaag atgcataatt taaattttta attttatatt ataaagctcc ttagacggag
    25381 cttttttttt taggtaaaat ttaaataccg gttttcttca gatcaaaatt atcaatcatt
    25441 ttaaataacc agagttttat tccaaattga ttttgagtag acttctctgc tgcatccttt
    25501 aatcttttga gatcggatct taaatccttt ttttttaccg aagtttttaa tctgatcagc
    25561 tcaccggtga tcctcatata gtcataaaaa gattctttgt aattttccct gactgaattt
    25621 tcccttgcaa gaaaatgtct gaaagaatct atcatagcaa ttgcctgatc ataaaaactt
    25681 tcttcataac tgatttgaag tatcaggatt tttacctgaa tctttattat gaaatttgga
    25741 aaattagtct gcgacaaaag ttcaagtgct tttaatctgt ttcctttcat atattcaatg
    25801 tatccataac taaaattaag agtgctttcc ttttcttcat taagctgatc tttgaatctt
    25861 gtaatatatt tctctgccca gtcaaattca tttacccttg ctgcaacttt tacatggttt
    25921 acaaaatcca gatacatgat ctttcccata actatagtat cagtttccag attatccttt
    25981 gaaagataaa aatgctcttt cataaaatca gtactgcagt ctgtattgta tctctcagca
    26041 caaaatcctg tcatatgcag gaaatacatg tatttctcat aaaatcccag actgtcaaaa
    26101 atttttttac cgtgatcctt caattcataa aaacttctat catcattttc tttgtgtaac
    26161 attatgatta aataaaacat cttcagaacc ggagcaatat tatctttgtt tttttccagg
    26221 tagatcaata cttcatcaaa catttccaat ttaaattcat aattgttttg tttgatctcg
    26281 tgcagcataa ttgtataaag atcaagagct ttaatcagaa aatgtgtcag ttcgtaatca
    26341 agctgttcct gtatcaggtt aaaatctgag tttggtcttt taggtgaata aaatgatttc
    26401 agctcatttt tgagatcagc catattagaa taataaaatt catcccggac atgattgctt
    26461 ttgatctttt gataatatac atcaaatagt ttttgaaaca ttttatcaag gtcacggtct
    26521 ctgagctgtt cgagaaggta caaagtattc tgatgctcat ttttttgaaa aaatattatt
    26581 ttcaaaaatt ccatactcac agccagcaga tctgatgaca gattccttat tttaaaataa
    26641 tcatattcct catcaggaaa aataatttta aaaacatttt tggcatctaa atttttatcc
    26701 tgaaattcag ggtaaaattt tatcagcaca tcgagtaaag aaatgacaga tttgttttta
    26761 ttaaagaaag gagattgagt aaattctctg aattgtttga tctctttttt tgaaaaagtc
    26821 tttaaaacct gtataagttt agaattatgc attagtcttt tttctaaaaa aattatttaa
    26881 aaagtgctaa atcagcttaa atttatcgat tatgaatata attatacact attaggatat
    26941 ttatatttct aaaaccgaat aaattattct tttgaaaata aaatagaatt ttataatttg
    27001 gatcacttgg cgtatgatta caatttcatt tttaacactt cccgggatta ttcttatctt
    27061 ttcttcgtac gccatacgtt tcttgataaa agaaaatttc gggaagtcta ttttaatact
    27121 tatgatttta aaaaaatatt caattaatat tataacgaaa ggaaaaaaat gaaatcaata
    27181 atttcacttg tcattatctg cgctttgtca gttattctta cggcaggcac agcggaatct
    27241 cagcaaaata caacaggtaa ttccaaaaaa gtaaaaacat cttcattcca taaaaaagga
    27301 aagaaggtta ctgaccaaaa aacaataaat gaaatcattt ccaaaaatca aaaatcttat
    27361 aaaaagaatt tacgcggcag ctggcttctc gaagaagatt tcgagtccgg aaattttcct
    27421 cctgccggct ggtcaatttt aaatggagca acagagtggg aacagcaatt ttacagtgga
    27481 tacggaaatg gcaattactg catgttttat tcaagctggc aatgtaatta ctcggataat
    27541 ataattttta caaatgattt taattcaaat acgcaattcg gagataagct tatttttgat
    27601 tacgcttatg ctccgtatga tgacggaaat gcttattatg atgatctgga aatttattac
    27661 tacgatgcag tagaacagat ttattatagt cttgtttatt attacggatg ggagttacaa
    27721 accgcaagcg taggtgatca gtattttgaa cctcagagca atgaatgggg aacgatgatt
    27781 gttgacatcc ctgaaaacgc ttcaagtata tatttcaaag tctgggaaaa ctgcgccaac
    27841 aatctttata tagataatat taaagttgga gctgttatcg gaggttatga tgcttcagta
    27901 gaaattttat ggggaaaagg aaaagttcct ctttcttacg gtgtgcctga taaaataccg
    27961 gtgctcatta aaaatctaac gagtaatcca ataaataatc taatggtaag cctggaaatt
    28021 tccggaacaa acaatttatc tgatgcaatc gaaattcctt atatcggacc tttagatact
    28081 tttcaggttg acttccttgg atttactccg gtgttaaacg gattttccga tattactgtg
    28141 tctattccaa atgatgatga taatggaaat aatgagaaga cttttattac agaagcaaat
    28201 tccaatacaa taaggtacgt tgattcaaac tgctgtaaca gcagtgtcgg ctgggtcgga
    28261 cagaatgctt ttctgaataa gtattatatg tcaggcacag gtcagataag aacagtaaac
    28321 ataaaacttc cggactggaa ttttggtcag atagtttacg gtattgtttt aaacagcagc
    28381 ggtacaatag tgggaaaatc accgcactat aaaataaaag cgtctgatca gaatacatat
    28441 aaatctttcc agatcacaga tcccaaaccg tttactgtaa ctaacgatta ttactatgtc
    28501 ggtatagctc agactgaata cgcaggagat ggttttgcat atggatctca gcagatgctg
    28561 tatgaattac ctgcaagacc tgacgcaaat tattattcta atcttgctcc tccgggtaca
    28621 aatgtcggtg cttatgaatt tccgagagag tggggacaga attacgcgat agaagctgta
    28681 atgggaaatc aggcggcaac agatgcggga atctccgatc ttggtctgac ttatgatcag
    28741 tattttaatt caactacttt tacgcctgta ggaaaagtat ttaatgcagg cacaggctcg
    28801 gtctcattca gcgtcagaag aactatttca cccggtggat atacaagcac taaatccgta
    28861 acaggattag ctgcaggagc aaatgcattt gtaaactatg atccgtggac atttacttcg
    28921 ggtacagttt atactgtgaa agattcaatt cttatttcag acggaaataa ttctaacaat
    28981 gtaatgacat ccaatattac tcctaaaatt gctaaggaaa tgtgcatact ttggcagcag
    29041 cagaaagaca gagacagtct ggtcagagca gtcatcagcg acggtagata tgctaataat
    29101 tttgatacag taggaatgta ttatacagga tcatacagag cctggaaagt aatgattgtg
    29161 aactttaaag aagaaggaag ttattcaccg tgggtcaggg attctttaaa aagattcatt
    29221 gacaactcta ctgccggaaa taaaaaatca ctgatcgttt ttagtgataa tacttcttta
    29281 ccaaatgatc ctgagaccgg atatatgacc ccggcagata caatatttta cagacaatat
    29341 cttaaagcaa agacaataag cgacgactgg atcggaagta ttccatcaag tcagagtaaa
    29401 ttcagaggag tcggattttt caacggtatc tcacaggatt ctgtatccga tccctacaca
    29461 cctgaattaa taagacctgt taacggcagt atagcagcat tcaagccaaa gtcagttaca
    29521 ggaaacggat cagactcttg taatgcagtt tgctttgcag gagcaaatta taatacattc
    29581 ttcatgacta atcaattttc aagtctgaga gcaaccggcg gatctccgct atcacttgga
    29641 cctgttttgg tttatacaaa aatcatagac tggctgcaga gtgtaaacac aaacgcaaaa
    29701 gtacttgacc tgacagcttt gtatgaaggt ctttacagca ttggtcaaaa tactatggca
    29761 ggtgatacag taactgtata tctgagaaac gcatcaagtc catatcagaa attagattcg
    29821 tcaaagggat ttctaaattc ggtgggatct gaatctttca ttttcaataa tgcttctaat
    29881 ggtgtaaatt attatctgca tctgaaacac agaaacggac ttgagacctg gagtaaaaca
    29941 cctcagcagt ttagtgcaaa ttatgcttca tataatttta ctgtaaatgc aaacaaagca
    30001 ttcggtgata atatgatatt aaaaggaagc aagtggacaa tatacagcgg tgatgtgaat
    30061 caggacggag tcatagacat agatgataac tctttgatag aaaatgctgc gtttaatttt
    30121 gaatcaggat atatccgtac agatctgaac ggagatgaaa tagcggatat cagtgatcag
    30181 gcgatagctg ataataatgc atataacttt gtttcaaagt ccactccggg tggagctctt
    30241 attgctcccg aaattcagga agattatgtt ccggttaacc aaagtccaga tactgaattt
    30301 aaaactgatc atgaaatata tgaaagaact aaaaacatac aaagtaatat taagtcaaat
    30361 gacttaaggg agaaattatt tatcaaaaca aataaataat ttatccagcg aaaatgttca
    30421 aaaaaactga ttcgtaaccc tcaaaaaaga ccggcagaat acagccggtc ttttttttta
    30481 ttcttagcct attccaaatt aaatttattt tccataaatt acataaatca tttttcaaat
    30541 aattcaatga tcatgcttaa aaaattacct ccttctttta tttcttcaga tagacttagt
    30601 ttgaaaattt ttataccggt attatttgca gtcttatact ttggaaatac aaatgtaaag
    30661 gccggagaca caacatgggt cacaactttt gatcagaatt ttcagaactg ggctgatact
    30721 cattatggaa attttttgct acccgatacg aatacacatt ataaaaaaat cacaatgtac
    30781 tatactatcg gctgtcctgc agcgggttgt gatccgtggg acaggattgg ctggataaaa
    30841 ctttatacag attctgtagc gaatgttgag attgcgagaa ttgtaacgcc atataatatt
    30901 gtaggaggag gaatgccggg acaatgtgca tttgaaattg acgtgactga ttatatgtca
    30961 ctacttcatg attcagtgag acttggaagt tatattgaaa gctggatagg aggtacgcgt
    31021 ggctggcttg taacagtaaa gtttgcattc attgaaggtg aacctgaaaa gattccatat
    31081 aaagttgtaa atctctggca ggataacaga attgtttacg gtgataccgc tgtgaatcct
    31141 gaagcgatac tcgtacctag agaagttgca gtagatatgg gcacatctaa agcaaaagtg
    31201 aaaataatta ctacaggaca cggtcagggt aacacagata atgcagcaga attttcttta
    31261 aagatccata cacttaaagt aggtgcacag aattacgatc aggtgctttg gagatcagac
    31321 tgttcagtta atccatgcag tcctcaggga gggacatggc agtatgcaag agcgggatgg
    31381 tgtccgggag ctagtgtgat cccgtgggat agtgacgtga ctgcaaatat aactccgggt
    31441 cagaaagtga taatagatta tgatctgtat ccttatgaaa atttctgccg accgacaaat
    31501 ccgatttgca tttcagggca gacgtgttct gattgcaatt ataataataa cggtcatacg
    31561 gaaccgcatt atacaattca gggacaactt gttttataca aagataatcc ggcaataagt
    31621 gtaaataata tcagcacaaa tacaccggag aatttcatgc ttcatcagaa ttatccgaac
    31681 ccgttcaatc catcaactaa gataaaattt gatctgccat catttggaaa agtaacacta
    31741 cagataattg acataaacgg aaagacagtt gatactttag tcgagggaaa attaaatccc
    31801 ggaacgtacg aagccgaatg gaatgcgggc ggattgtcca gcggagttta tttttataaa
    31861 ttggagtcgg gaaatttttc acagacaaac cgtatggttc tgatcaaata attacaagta
    31921 tcatcagact attaactgat atttaatatt gaaattaata taaatttgaa ttctacactt
    31981 aataaaaatt gaagtttgct ttataaaaaa ttttttatga attttataaa ccataattgt
    32041 aaaagttaaa cagctcagaa aggaggttgc tctgaataaa ttgaaattga tttgaaattt
    32101 aattt
//
LOCUS       DAORVQ010000014        31519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_454_length_31519_cov_6.923977, whole genome shotgun sequence.
ACCESSION   DAORVQ010000014 DAORVQ010000000
VERSION     DAORVQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 31519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31519
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_454_length_31519_cov_6.923977"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLX80_02365"
     CDS             complement(<1..891)
                     /locus_tag="PLX80_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY19670.1"
                     /translation="MSVLIKNGRVITSSEDYIADIFIEGEKISAIGKDLNIPADEVMD
                     ANGKLIFPGGIDPHVHLDMPFMGTFSSDNYETGTTAALHGGTTMVIDFVLQTQGKSLY
                     SALGEWQSRSDGNALSDYSFHMAVTDFNKDTKKEIGEMINKEGITSFKTFMAYKGALM
                     IDDRMMIGLMNEVRDKGGVVTVHATHGDMIDFLIAKHREEGKLSPLYHYLSQPEITET
                     EASGRFADMAYYTGVPAYIVHMTCEGALNQIRRATERDQKVFVETCIQYLLLDASLYE
                     KDFEGAKWVMSPPLREKKDQK"
     gene            complement(917..2419)
                     /locus_tag="PLX80_02370"
     CDS             complement(917..2419)
                     /locus_tag="PLX80_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HQY19671.1"
                     /translation="MIKDQSYEPVNSPSASLYSEDLAPVPESKRTWNTWNYAALWISM
                     SLCIPTYMLASSLIEGGMNWWQSILTIFLGNTVVLIPMILNGHAGAKYGIPFPVFARS
                     SFGTKGANIPAILRAIVACGWFGIQTWIGGFAIYLMIATWMPSLTQLPSIFPESFGLE
                     TGPAICFFLFWLLNMYVVYLGVDSIKKLLVFKAYFLPLSALALLYWAVSEGNGFGPIL
                     SQPDKFQSSAEFWNFFFPALTGMVGFWATLSLNIPDFTRYAKSQKAQVMGQIIGLPPS
                     MTLFSFIGVVVTSATVIIYGNTIWDPVVLAGKFENKIIVAVAMICIAVSTLATNIAAN
                     IVSPANDFANLVPSKIDFKKGGYITGVLGILIFPWKLISDPNGYIFTWLIAYSSLLGP
                     VGGIMIADYYFIRKKILKVNDLYSVTGIYTYFKGYNRAALIALIAGIIPNVPGFLTTV
                     HLVSENTFPQVIDNLYHYAWFVGFGISFTVYLFLMKNRKGNQEVTSHNTN"
     gene            complement(2800..4149)
                     /locus_tag="PLX80_02375"
     CDS             complement(2800..4149)
                     /locus_tag="PLX80_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY19672.1"
                     /translation="MNSTTLSRNEIINLSLQHTITSWSKQKGISPPVINKAEGVYFYD
                     MDGKKYLDFSSQLICVNIGHGHPKVLDAVSEQMKQVSYINPSMTTEARAVLGKKLADI
                     SPGNLNRTFFTLGGAEAVENAVKLARLYTGRHKIISLYQSYHGATYTAFSAGGDPRKH
                     AADSQQAPNFIHVENPYFYRCPWNSKTPQECTENALAHLERIVQYEGPQNVAAIILEG
                     ESGSSGCIKLTPGYWKGVKAIAEKYGFLTIDDEVMSGFGRTGKWFGIDNHDTAPDIMC
                     MAKGITSAYLPLGGIMISEEIAKTFNDKPLPLGLTYSSHPVTLAAANAVLEIYEEEDL
                     INKAAEMGTYVNQKMCKMMGKHPSIGDFRNTGLLGCIELVKNRETKEPMAKWNASPDE
                     MGVMPKVTAKLSELGMYTISRWNLIFVAPPLCITKEQVDEGMDIISEALKIADQEVS"
     gene            complement(4200..4637)
                     /gene="tnpA"
                     /locus_tag="PLX80_02380"
     CDS             complement(4200..4637)
                     /gene="tnpA"
                     /locus_tag="PLX80_02380"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQY19673.1"
                     /translation="MNYQQINICIHAFFGTKDSLPMIKESIEKELYNKIFSKITTEYK
                     SRVININGTEDHSHILFLLNPIYCPADILKNIKGETSHWINQNELTEEKFSWQKGYFA
                     HSVSENEIDKISRYIDIQKEFHKQYSFKDDCDIFLKKYGRFGI"
     gene            complement(4962..6422)
                     /locus_tag="PLX80_02385"
     CDS             complement(4962..6422)
                     /locus_tag="PLX80_02385"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQY19674.1"
                     /translation="MNQQIPEVKNYISGKLTKNGHRTMEVICPSDGKIISTVPMSTMK
                     DLDEAVNSAKEAFPGWSAIPIKERVQVFYRYKTLLEKNLKELAELTHIENGKTMDESI
                     AEVEKSIELTEFACSLPQLVGGEIMEVSSGVECRIEYKPIGVVASIAPFNFPHMVPHW
                     TIPNAIALGNCMILKPSELVPISANRIAEMLKEAGLPDGVFNVVNGDREIVEAICDHP
                     GIEAVSFVGSTKVAKIVYKRATSNLKRCIALGGAKNHLIVLPDAHPEMTANNVAASMS
                     GCAGQRCMAASAMVGVGEIDSIVEKICDEARKIIPGENLGSVISKEAKERIERYITDA
                     ENEGAKVLVDGRKTSVKGKEEGFYVGPTVIDFVKPEMAIAKEEVFGPVLAIIRVNDID
                     EALKIENSSEYGNAASVFTQSGGAARYIMDKASAGMIGVNIGVPVPREPFSFGGWNES
                     KFGVCDITGKSSIEFWTLQKKTTTKWNAEAKRNWMS"
     gene            6581..7504
                     /locus_tag="PLX80_02390"
     CDS             6581..7504
                     /locus_tag="PLX80_02390"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQY19675.1"
                     /translation="MANVSKQYFILFLFLFLISVGNVFPQDIPPKILVVTAHPDDDAL
                     FAGANYKIIHDLKGTVDLALITNGEGGYKYSTLAEDYYGIELTIEDTGRKYLPEIRKK
                     ELEAGGAIIGIRNYFYFDQKDNRYITTENIREILDSNVWDLDYIRSKLKDILIKEKYD
                     FVFIMTPTEGTHAHHSSSAILALQTIQSLDEGARPIVLAGSGSSLSDTVKNIYKGLKG
                     FPITNVSDTIPFQFDRTQKFGFREVLDYKIIVNWLIAEHKSQGTMQLGMNRGDIENYW
                     YLDINPESGKIKTQELFDKLKINYYPKKEYD"
     gene            7508..8533
                     /locus_tag="PLX80_02395"
     CDS             7508..8533
                     /locus_tag="PLX80_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQY19676.1"
                     /translation="MKILSPLLILICLILITGCSKDSRKKLTVYSPHGKEMLTEFEQR
                     FEAANPDTDIQWLDMGSQEIFDRIKTEKENPICDLWWGAPATIFMRAEKEGLLEKYIP
                     TWAESIPISSKSKDGNWFGTYLTPQVIAFNDKTLTNEKAPKDWEDLINPEWKDKIIIR
                     NPMASGTMRVIFSAAIANSIKTTGNDKEGFDWLRKLDENTSDYAADPTQMYIKLSGEK
                     EAVTLWNMPDIILQKTLYNYPFGYNFPVDGTVVLTDAIAIINGSKNPEGAKEFYEFVT
                     NEESLIIQSEKFFRIPARNDIPKDKLPEWVRQADYKTMDLDWELISENESGWMKLWDT
                     EIKGKSR"
     gene            8572..9591
                     /locus_tag="PLX80_02400"
     CDS             8572..9591
                     /locus_tag="PLX80_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19677.1"
                     /translation="MANLEIRNITKKFNDTEVLSDMNIDVRQGEFFSIVGPSGCGKTT
                     LLRIIGGLEFPDSGKIIVNGKDITSLPPQKRKIGIVFQNYALFPNMTVYQNIAYGLEI
                     LKTDKSVLNNKINSVLDIVSLSHKTNNSVTSLSGGEQQRVSLARVIVTEPELILFDEP
                     LSNLDYSLRIETRNELKRLQRDVGITSVYVTHDQTEALALSDRIAVLNKGVVQQTGSP
                     YEVYYNPKNSFTASFIGHANLFDEKNSEQIFKINTGKNKVLALLPEEIILTKDDEEGK
                     GKITDVQFTGFSIEYSIQIINSDRIIKTLCTTTSETEHLKTGDRVSLKISENNRDSNL
                     RILDK"
     gene            9591..11264
                     /locus_tag="PLX80_02405"
     CDS             9591..11264
                     /locus_tag="PLX80_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQY19678.1"
                     /translation="MKKLSSVNKFLLAVIIFFTFGYIIYPMISLVRESFTLADGSFTP
                     TAFLNAFSPSSVSATLNSILLSVITVIGSAFIGILFAYIFQYHRIPLKSFFSSIIIIP
                     VATPPLIGVVAFLFLLSENGLISKLLTFVFGMNGSLFSFDGWAAIITIHVYSFYPLFY
                     LFVSSALKKIDGNTIDASYSLGASKSKTFFNVIVPQLIPSMIGASLIVFMASMASFSA
                     PFIFGGSERFLTTEIYSAKINGDNSAASSLAVMLTIISLLFLLLLRFYRSRKTYEIRY
                     KGTPRTVITGHKKGINILSSTFTVIFSFLIVLPIFTLLFLSFIPEGSLMRNFFAEPFT
                     FGNYSKIFGEAQFFDPFINSLQMSVIAVAITVVIGVGTAFLIVKKNVKGGNYLESILS
                     LPYGIPGTVIALAFILSFNFPSVFTGFIALVGTFWILPLAYAVRNLPILMQSSISGFH
                     SIDPSLEEASFSMGATGVRTFRKITGPLIFPAVLNGALLVFINSIGEFVSTILLYTYS
                     TKTISIEIYSQLRMYNTGAAASYGIILFLIVMIVVYFSRKTLDKSVGIS"
     gene            11401..13200
                     /locus_tag="PLX80_02410"
     CDS             11401..13200
                     /locus_tag="PLX80_02410"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19679.1"
                     /translation="MKTILRFIFIISVLIFSVNASIALNGSYTVGAGGDFPTIDSAVS
                     TAQAQGLGGPVIFNIKSGIYNEEIILQNIFGLSFTNNLTFQSETGNAADVIINGNVFV
                     FQVKASFVFFQNLTIGSSSQTSFQIYSSYTTIKNNIFTNDSKGILTFDQFPPRNIEIT
                     GNTNVPGIGLNPWRDYQGQNVIIKNNSVSGGIVVYFYLNILIENNITKFINTSSSSNA
                     TISKNKVNGTIECGGGYYNIFNNFLFSDSPNDSAIFSNAIGNVIYNTFSYSSRTSIIL
                     FGDSTTFLNNLMINKSGRYSFYVYSGGPFFGNPKYSDYNNFYNNGNTGLIKWHNDDFD
                     NVSSYFNATGNDEHSNSHPVTFVSPTDLHLAGSSLGDQQLAGIPTSLVLDDIDGQPRD
                     PSHPYKGADEYIDFPLPVELASFTSSVSNNRVNLKWTTSSEINNSGFDIERSFLKNSI
                     SEDWLKVGSQTGNGTTNYQSSYEFNEKDLLPGKYYYRLKQIDYNGNFRYHNLTGEIVI
                     GIPDKFTLSQNYPNPFNPKTVISFGLQLAGRISIKIYDVNGKEISSLVNQSMEAGFHS
                     TEFDGSNFSSGVYFYVLNFEGKNIDRKRMVLLK"
     gene            complement(13272..13427)
                     /locus_tag="PLX80_02415"
     CDS             complement(13272..13427)
                     /locus_tag="PLX80_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19680.1"
                     /translation="MSFKFVTPQNPKNTRLFGDFELNVSIYKTAESEKHVTLRGQFLS
                     DFAEIVL"
     gene            complement(13424..13906)
                     /locus_tag="PLX80_02420"
     CDS             complement(13424..13906)
                     /locus_tag="PLX80_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQY19681.1"
                     /translation="MKKVVIEKEVLINATKEKIWEVLTEDKPYRIWTAEFCEGSHFQT
                     DWKEGSKILFKTPTGEGLVSKLMECKPNEIISMEHQGILLNNEEVYDNEEAEKWKGMK
                     EIYSLSGKDGATVLSIYQELEKSYEEWSTYAWDKALFILKELAESGHSNYYEKIVNPG
                     "
     gene            complement(14021..14893)
                     /gene="blaOXA"
                     /locus_tag="PLX80_02425"
     CDS             complement(14021..14893)
                     /gene="blaOXA"
                     /locus_tag="PLX80_02425"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HQY19682.1"
                     /translation="MKIILFILSISLLISCSPPKKSDQISNENSDLVSESDNIHHDKI
                     TEEYREDFQMIIDSVGLIGSVLIFDPQSKIYYSNDFKRCSTGFIPASTFKIPNSIIAL
                     ETGVAKDDSTVFKWDGKKKFLPQWEKDLILKDAFQFSCVPCYQQIAREIGTVKMKDYL
                     KKLDYGNMVVDSSNIDTFWLTGESKISQMQQIDFLKKLYNLELPISVRTSETMKRIMI
                     IDENDIYKLSGKTGWAVSGENNLGWFVGYIETKKGIFYFATNVEPVIGFNMDEFAGIR
                     KDITLKAFRKIETI"
     gene            complement(15024..16514)
                     /gene="carB"
                     /locus_tag="PLX80_02430"
     CDS             complement(15024..16514)
                     /gene="carB"
                     /locus_tag="PLX80_02430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQY19683.1"
                     /translation="MPKRTDIKTILIIGSGPIVIGQACEFDYSGTQAVKALKEEGYRV
                     VLINSNPATIMTDPEIADATYIEPINSKYIEMIIRKEKPDAILPTMGGQTALNAAMDL
                     HEKGILQKYNVELIGAKVDSINKAESRELFHNAMKKIGLNVSNGKFIKNFEDAKEFAD
                     KTGFPLIIRPSFTLGGTGGGIAYNVEEFETIVNKGLDASPTHEVLVEESMIGWKEFEL
                     EVMRDVNDNFVVICTIENMDPMGVHTGDSITVAPSQTLSDRQYQELRDAAKKIISEIG
                     VETGGSNIQFAVDPDTGKVIVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITKTTPSCFEPVIDYVVVKIPRWDFDKFRSTNPDNDVLGVQMKSVGEVMSFGRN
                     FKEAFQKAIRSLETGRAGLGADGKDKYEFDQIMNLSTEEKISLRSKTLIKLQTPRSDN
                     IFYIRYALLLDATTEEIFQATKIDKWFIEQIREIIETEKEIRSYSF"
     gene            complement(16560..18701)
                     /gene="ppk1"
                     /locus_tag="PLX80_02435"
     CDS             complement(16560..18701)
                     /gene="ppk1"
                     /locus_tag="PLX80_02435"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQY19684.1"
                     /translation="MSKKKSEYFLNRELSWIEFNKRVLEEAKDSSQPLLERLKFLSIF
                     STNLDEFFMIRFAGLKRQITSQVNDLSTDGMTAQEQQDKVFEVLTPQIEDLHRIFNDE
                     IKPLLKSNKIEFLNYEKLNDEKKSVLDKYFDQELFPVLTPLAIDNVHPFPNLINRSLA
                     LAILLDDPDTEVVEEKVCVIQIPNNISRFYLINNGTEYHFILLEDIIKANAVKLFPGM
                     KVIDSYAFRITRNADLELEEEEAADLLTLIEEEVKKRRLGILVRLEVDNKMPDILLAF
                     LKNILKAENNEIYKIDGMLNLGDLMSLYKIDIRELKYEGFTPRISSYFRQEEDFFKAI
                     SENDIMLHHPFYSFSSVSEFIAQSAEDPNVYAIKLTLYRTSGDSLIIKSLIEAAENGI
                     QVTAVVELKARFDEENNIIWARELENAGVHVVYGLVGLKTHCKMALVVRKEKKGMKRY
                     LHLSTGNYNSVTSRIYTDFGLFTTDQDFCKDASNLFNYLTGYSKQKSFNKLLVAPITL
                     RRKVLKLIDDEIKSHKENNNGYILFKNNSVVDEEVILKLYQASKAGVKIDLITRGICR
                     LRPQVEGLSENINVYSIVGRFLEHSRAYYFHNNGEPKLYFGSADIMQRNFDRRVEIFF
                     PIEDKRIKQDAIDILKLYLTDTAKTWVMLDDGSYIRKEDFLKLNDIQFEKKNIQEFLL
                     KKAVIKTEKELKESLGKKVIHNGVHKKKETV"
     gene            18827..21169
                     /locus_tag="PLX80_02440"
     CDS             18827..21169
                     /locus_tag="PLX80_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19685.1"
                     /translation="MGKNIFSLVKFTTYLICTFLLFSLASNSKAVTITEDLDPETTLW
                     LQRVSAAGGSVSDSIVSAVDDYIKEIKGLKYQSLNIRDRIFRENWFCGNFNAAFVPII
                     VNPTGSPLAMGNPTDVNRNFTSSNYKDTGKYSGLKGNGVNQYIETGFTPSLINEFPLN
                     DVYFLFYCLTDSVDNGRSGSRGTSGSGFYAYPKFITGNSHFVLNSNTGGVVVFQSIKG
                     YTLHQRSNSQEIKSYYSLNQVGTSQVVTTSKPDKQIYICAFNNNGVTDTYSTTRYGGY
                     SFGKSFSEQARVVHFNAVQRLMSRLGRKLPESIQTDLKSKLMTFTGKNLIVNYETRNN
                     GNIKFGLSDINGNVISGYSVDNCLPLTGNEFTKTVSWNSGNDLSSFVNTPLYLNIRMT
                     DADLYSIQFQNQIVQTDSSKQGFKIGTYKQFFADTLFIGSFPSVMRKMHNPVKDPQPV
                     VRPEQEWEENRLFTTYSNVTYSKSIEQNRMVFKMWLRAVTGTYRLPVYYESVDGVNWT
                     RPVLEQFKFNNSMENNIMSDAPYPGGLYTVVDDSLYNQSDSTRRYKSMFNTHSNGLSY
                     LNVSFSYDGITWIPYADNPVGYMGEDLTSGGWNPVIGKYLGYFRDSLGIRNVSRYVSN
                     DWINWTKTGKVLVPDANDQAVTGYYNLHVLFKDSVYWGFLGHLQLNSNGDENPPNPSR
                     TDNTVHIELVFSRDGINFTRCGNRMPFLDIGPLAAWDDQCVYSIGVPIVVGNEYYYYY
                     NGFNTKHMWTAPPPANGQPKTAQVGLAKIGLDRFISLSTY"
     gene            complement(21597..22088)
                     /gene="msrB"
                     /locus_tag="PLX80_02445"
     CDS             complement(21597..22088)
                     /gene="msrB"
                     /locus_tag="PLX80_02445"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQY19686.1"
                     /translation="MLVTLFSCSKKEEVTLGNFQDSIKSQVKEESTSEFEIVKTEEEW
                     QKILTEEQYRVTRQKGTERAFTGEYDKNKRSGKYLCIGCDQELFESNTKFDSGTGWPS
                     FYEPFSKENIIVGVDNSKGMSRDEVLCSRCGAHLGHVFDDGPAPTGLRYCINSVSLKF
                     VER"
     gene            complement(22197..22592)
                     /locus_tag="PLX80_02450"
     CDS             complement(22197..22592)
                     /locus_tag="PLX80_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19687.1"
                     /translation="MTNEIRESLLKSEKNYIKLKKTNSRFIMISLIASVLATLLAGLT
                     AFTGPVAGQGAVAWKWTCGIVAVFTATATIFTGMHKQLSVSDHLTKSLECTGKLRSLS
                     FTIQQEGYNKEEAVKEYKRILELYSEYLV"
     gene            22805..23443
                     /locus_tag="PLX80_02455"
     CDS             22805..23443
                     /locus_tag="PLX80_02455"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQY19688.1"
                     /translation="MAALRLGDTAPDFTAKTTEGEIIFHKWLGDKWGVLFSHPADFTP
                     VCTTELGMVSKLKDEFDKRNVKAIGLSVDPLDSHIKWINDINETQNTTVNFPMIADEN
                     KYVANLYDMIHPNADNTLTVRSVFIVGPDKKIKLTLTYPASTGRDFFEILRAIDSLQL
                     TANYSVATPVNWKDGDDCIIVNSISNEDAVNKFPKGFKEIKPYMRVTPQPNK"
     gene            23698..24378
                     /locus_tag="PLX80_02460"
     CDS             23698..24378
                     /locus_tag="PLX80_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HQY19689.1"
                     /translation="MKYILIAILCVSFLNDGIAQTDGVSKNSSPFKIARLKYSGGGDW
                     YNDPSAEINMMEYLKKNTVIDVDEPKFYSVDVASDDIFNYPFILITGHGNIELSQSEA
                     ARLRKYLESGGFLYADDDYGMNESFRKELKKIFPDDELQELPFSHEIYNSHFSFPGGL
                     PKIHEHDQKPPQGFGIYKNGRLCVYYTFETNISDGWADTKEHQDPPEKKEESFKMGTN
                     IIVYSLMN"
     gene            24418..25323
                     /locus_tag="PLX80_02465"
     CDS             24418..25323
                     /locus_tag="PLX80_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY19690.1"
                     /translation="MILKKYPDSIPNVKDNFIVHFTSHSSELDRHITPLTVKCSMKGL
                     EKFHTSESSYGVSPASFLILNEGQQCESSIINESESFSIYFEPRFANSALKSLITPSD
                     KMLNHSFKPVSQPVHFFEKLYPHNNFLSPVIMKMRLASKVSYDDENWLKERYLEMLDG
                     LLKVHKDLYKEIEKLPPVKLSTKTELYRRVSIAKEYIDLEFTNDLTLDKISKKACLSQ
                     FHFLRIFKSVYKQTPHQYLTKKRIDKAIDLLRKSDMSVTEICFEIGFESVGSFSWLFK
                     NKFGLSPETFKESYRRTMMKFKLLK"
     gene            25491..25853
                     /locus_tag="PLX80_02470"
     CDS             25491..25853
                     /locus_tag="PLX80_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19691.1"
                     /translation="MANSINWFEIPVTDFGRAKKFYSEIFGIEMHEEMMGPHQMGFFP
                     GEGVSGAIIKADGYKPSMDGALVYLAGGEDLTGVMNKIESAGGKILQPKSKVTDEIGY
                     IAIFADTEGNKVALHSPK"
     gene            26021..28078
                     /locus_tag="PLX80_02475"
     CDS             26021..28078
                     /locus_tag="PLX80_02475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQY19692.1"
                     /translation="MRSAFFILLISLAGFFSLNEIAFPQSSNDLKSGLDPENINNSID
                     PNSDFFDFACSNWLVKNPIPAESSRWGTWDVIIEKNNEVLRSVLEKASSNSSAPKGSI
                     EQKLGDLYFTSMDTVKIEKEGIAPLADYLAMIDGISNKDEFQKVLAYLKSIGTGGVFS
                     FRAGQDDKNSNDVILQLFQGGLGLPGKEYYTKDDENSIKTREKYMEFMNTIFVLMGND
                     NSTAGNISRRIMDIEKRMSDASMTRVEMRPAEATYHLMTLKEVRDLMPDYNFDVLFAE
                     IGLENNSKFEKGINVGQPEFFKEINRMLTDVSLDDWKNYLKWNLVRNNADKLSTDFEK
                     ANFEFYGKTLRGSEVQKPRWKKSISFVESAMGEPLGQLFVEIRFKPETKTKALEMVNN
                     IKESFKDRINQNEWMSEVTKKEALKKLSTFNVKIGYTDEWKDYSGLEIDRVSFLDNMI
                     RASEYRIKLNLDKIGKPVDKNEWFMNPQTVNAYYSASNNEIVFPAGVMQPPFFDPEVE
                     DAVNYGGIGAVIGHEISHGFDDQGRKYDGDGNLKDWWTEEDADKFKVRAEMLAKQYSG
                     YQAVDSYYVDGNLTLGENIADLGGLITAYHAYMKTIDYNDDVKIDGFTPSQRFFLGFA
                     QIWRGDYRPEMLTMLVKTDSHSPPKFRVNGTIYNIPEFMKAFGGKEGDPMINSKDKIV
                     VIW"
     gene            complement(28221..28832)
                     /locus_tag="PLX80_02480"
     CDS             complement(28221..28832)
                     /locus_tag="PLX80_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQY19693.1"
                     /translation="MSNIITKELIENAFSYDTYKEIVRKLILKNKTSGNNQSESNIYF
                     TKLNAERVKRIEKTTVLNEELKRQLDKINSPLIVLVIAEAWCGDVAQNLPPLKVISDF
                     NPNVDVKIIFRDENPEVMDQFLTKGTRSIPVFLLIKKDSLEVIGKWGPRPEPAQEIMR
                     EAKSRKDYSHDEATKEIQLWYAKDRTLTFQKELSDFLNIISSD"
     gene            complement(28956..29993)
                     /gene="queA"
                     /locus_tag="PLX80_02485"
     CDS             complement(28956..29993)
                     /gene="queA"
                     /locus_tag="PLX80_02485"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQY19694.1"
                     /translation="MKLSQFKYAIPKNLIAKNPKSPRDACKLMVINKETGDIETKKFK
                     DIINYMESGDVLVLNDSKVFNARLYGTKEKTKAKIEVFLLRQLSEDENIWDVVVDPAR
                     KVRIGNRIFFTKNLYCEVIDNTTSRGRIVRFNYNGDFSKYIEKLGRTPLPPYIKREST
                     EKDKEDYQTVYAKNIGSVAAPTSGLHFTKDLLKKIEAKGIKIAFITLHIGLGTFRLVE
                     VEDLSKHRMDSEYYELSKDSADIINAAIQKKGRKVIAVGTSVARVLESNVVTSKHVRY
                     GKGWTDKFIHPPQSVRVIDKFVTNFHLPQSTLLMLTCSIADRDLVMKAYKKAIKEKYR
                     FYSYGDALIII"
     gene            complement(30027..31313)
                     /gene="eno"
                     /locus_tag="PLX80_02490"
     CDS             complement(30027..31313)
                     /gene="eno"
                     /locus_tag="PLX80_02490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQY19695.1"
                     /translation="MPVIHKVHAREILDSRGNPTLEAEVILEDGTIGRAAVPSGASTG
                     EREAVELRDGDKNRYLGKGVSKAVGNIKNTIAKKINGMNSLDQNLIDEVLIKLDNTKN
                     KSKLGANALLGVSLAAAHASANYLKMPLYKYIGGVNAKTLPVPMMNIINGGKHADNTV
                     DFQEFMIIPAGAKSFSEALRYGTEVFHSLRNVLHKKNYNTNVGDEGGFAPNLKSNEEA
                     IEVIMEAISKAGFKAGKDIYLGLDVASSEMWKNGKYNFYKSHIPDKTSEQMVKLYEKL
                     VKDYPIISIEDGMAENDWAGWKLLTDALGKKVQIVGDDLFVTNTEILQKGIEKKIGNS
                     ILVKVNQIGTLTETLNAIELAKINNYTAVISHRSGETEDTTIADLAVATNAGQIKTGS
                     LSRTDRVAKYNRLLRIEEELSSTAVYPGINAFYNIK"
BASE COUNT         9842 a         5702 c         5384 g        10591 t
ORIGIN      
        1 tttttgatct ttcttttctc tgagcggcgg agacattacc cattttgcac cttcgaaatc
       61 tttttcatac agagatgcat cgagcaacag atactggata catgtttcca caaaaacttt
      121 ctgatcacgc tctgttgctc tgcggatctg attcagtgcg ccttcgcaag tcatgtgaac
      181 tatgtaagcc ggtactcctg tataataagc catatctgca aatcttccgg aagcttcggt
      241 ttcagtaatt tcaggctgag agaggtaatg ataaagcggt gagagttttc cttcttccct
      301 gtgtttcgcg atcagaaaat caatcatgtc tccgtgcgtt gcatgaacag taactactcc
      361 tcctttatct ctgacctcat tcattaatcc gatcatcatt ctgtcatcga tcatgagcgc
      421 gcctttgtat gccataaaag ttttaaaaga agttatgcct tccttattaa tcatttcgcc
      481 tatttctttt tttgtatctt tattaaaatc agtgacagcc atgtgaaatg aataatcact
      541 taaggcattg ccatcggatc tgctttgcca ttctcctaga gctgaataaa gagatttgcc
      601 ctgagtctga aggacaaagt ctataaccat agtcgtcccg ccgtgcagag cagcagtggt
      661 tcctgtttca taattatcac ttgagaatgt acccatgaac ggcatatcca gatgaacgtg
      721 cggatcgatc ccgccgggaa aaatgagttt tccatttgca tccatgactt catctgccgg
      781 tatgttaaga tcttttccga tggctgaaat tttttcccct tcaataaaaa tatctgcgat
      841 ataatcttca gatgaagtaa tgactcttcc attttttatt aagactgaca taatttatat
      901 tttatttttt ttaatattaa tttgtgttgt gtgaagtaac ttcctgatta ccttttcgat
      961 ttttcattaa aaataaataa actgtaaatg atattccaaa gccgacaaac catgcatagt
     1021 gatatagatt atcaatgacc tgcgggaaag tattctcgct caccaggtgg acagttgtta
     1081 aaaacccagg aacattcgga atgatccctg caatcaaagc aatcagcgca gctctgttgt
     1141 atcctttaaa ataagtataa attcctgtaa cactgtatag atcattcacc ttcagaattt
     1201 tttttctgat aaaataataa tcagcaatca tgattccacc aaccggtccc agcaggcttg
     1261 aataagcgat cagccatgta aagatatagc cattcggatc ggagatgagc ttccatggaa
     1321 agatgagaat gccaagtaca cctgtaatgt aaccgccttt tttaaaatca atttttgaag
     1381 gaacgagatt tgcaaaatca tttgctggac tgactatatt cgcagcgata tttgtagcga
     1441 gtgtagagac agcaatacat atcattgcaa cagcgacaat aattttattt tcgaattttc
     1501 cagcgaggac gacgggatcc caaatcgtat ttccataaat aatcacagta gcagatgtaa
     1561 ctacaactcc aataaatgag aacaaagtca ttgaaggcgg gagacctatg atctgtccca
     1621 taacctgagc tttctgactt ttggcatagc gggtaaaatc gggaatattt aaagaaagag
     1681 tagcccagaa tcctaccatt cctgtaagtg ctgggaagaa gaaattccag aactcagcag
     1741 aggattgaaa tttatccggt tgggataaaa tcggtccgaa accatttcct tctgaaacag
     1801 cccagtataa caatgcaaga gcagatagag gaagaaagta agctttgaaa acaagtaatt
     1861 tttttatact gtcaacaccg agatatacta catacatgtt caaaagccag aaaagaaaaa
     1921 aacaaattgc cggaccggtt tccagtccga aagattcagg aaatatcgat ggaagctgtg
     1981 taagtgaagg catccaagtt gcgatcatca gataaattgc aaagccgccg atccatgtct
     2041 gaataccgaa ccatccgcat gctacaatgg ctcttagaat tgcgggaatg tttgcgcctt
     2101 ttgtaccgaa gcttgatctt gcaaagacag gaaatggaat tccgtatttc gcaccggcat
     2161 ggccgttcaa gatcattgga ataaggacga cagtattacc aagaaaaata gtcaggatag
     2221 actgccacca gttcataccg ccttctatca acgaactagc gagcatgtaa gtcggaatgc
     2281 aaagactcat actgatccag agtgcagcat aattccaggt attccatgtg cgtttggatt
     2341 cagggacagg tgcaaggtct tcgctgtaaa gtgaagctga tggagaattt acgggttcat
     2401 aagactgatc ttttatcatt aaaagtataa attttttcta taattaattc agtattctaa
     2461 tttaattaag agtccgtaga gcactgtttc tgccgaatta aaattttctg ttaatatttc
     2521 tttccgcgac aacccttttg ctcccccttt ttttgagact gtctcaaaag gatgcttttt
     2581 ccaaattttc cgattgggaa agttgaaata tagctcagag tcacatataa attttttata
     2641 aagagcgtat tttcttactt ttgagacagt ctcttttaag ggggaattac ttttacttaa
     2701 atctgaatag tgctattaaa tattttggca tattccaaaa aaaaatcact gaaaattttc
     2761 ttacaatttt gcaattaatt taggttgtat tcgaatattt tacgaaactt cctgatcagc
     2821 aattttcagt gcttcggaaa ttatatccat accttcgtcg acctgttctt ttgtaataca
     2881 aagcggagga gcaacgaaga tcagattcca gcggctgata gtgtacattc caagttcaga
     2941 taattttgca gtcacttttg gcatgactcc catttcatct ggagaagcat tccattttgc
     3001 cattggttct ttagtctcac gatttttaac aagctcaata cacccgagca gacctgtgtt
     3061 tctgaaatct cctatggacg gatgcttacc catcatcttg cacatttttt gattgacata
     3121 cgttcccatt tctgcagctt tattaatgag atcttcttct tcataaattt ccagaactgc
     3181 attagcagct gcaagagtaa cagggtgtga agaataagta agtccaagcg gaagaggttt
     3241 atcattaaaa gttttcgcaa tttcttcgga gatcattatc cctccgagcg gaagataagc
     3301 ggaagtaata cctttagcca tgcacataat atcaggtgct gtatcatgat tatcaatacc
     3361 gaaccatttt ccggttctgc cgaatccgct catgacctca tcatcaatag taagaaaccc
     3421 atatttttcg gcaatagctt ttacaccttt ccagtagccc ggagttaact tgatgcagcc
     3481 tgaggaaccg gattctcctt cgagaataat agcagcaaca ttctgcggac cttcatactg
     3541 aacaatcctt tcgagatgtg ccagagcatt ttcggtacat tcctgaggag ttttactgtt
     3601 ccatggacat ctgtaaaaat acggattttc cacatgaata aaattcggag cctgttgtga
     3661 atcggcggca tgtttacggg gatcaccacc tgcagagaat gctgtataag tcgcaccgtg
     3721 ataagactgg tatagagaaa ttattttatg tctgcctgta tatagcctgg caagtttaac
     3781 agcattttca actgcttcag caccgccgag agtaaagaaa gttcggttca gatttccggg
     3841 tgaaatgtca gcaagctttt tacccagcac agctctagct tcagtagtca tacttggatt
     3901 aatatagctg acctgcttca tttgttcgga gaccgcatcc agaactttag gatgaccgtg
     3961 accgatgttt acacagataa gctgagaaga aaagtcgaga tatttttttc catccatgtc
     4021 atagaaataa acaccttcgg ctttgttaat gacgggaggt gagattcctt tttgtttaga
     4081 ccatgaagtg atggtatgtt ggagacttaa atttattatt tcgtttcggg agagggtggt
     4141 tgagttcatt aagtattttt atatagttag aaatttttta aaaaccgttt taacggtttt
     4201 tagatgccaa agcgaccata tttttttaag aatatatcgc agtcatcttt aaatgaatat
     4261 tgtttatgaa actccttttg gatatcaata tatctgctga ttttatcaat ttcattttcg
     4321 cttacggagt gagcgaaata acctttctgc catgaaaact tttcctctgt aagttcgttt
     4381 tgatttatcc aatgtgaagt ttcacccttt atatttttta gaatgtctgc aggacaataa
     4441 attggattta gtaaaaataa tatatgggag tgatcttctg tgccattgat attaattact
     4501 cttgatttat attcggtagt tattttagag aaaattttat tatacaattc tttttcgatt
     4561 gattctttga tcattggcaa actatctttt gttccaaaaa aagcatgtat acaaatgttg
     4621 atctgttgat aattcatcgt tctcctttca ttttaataaa accgtttaaa cggttttatc
     4681 atattatttc attgataata ataaaatcca atctgtttct tatactaaac tgttacaata
     4741 aaatcttatt ctacattctt aaaactcgtt aactatttct tcgacaaaaa ccgataaatc
     4801 ggtttttcca taatatccaa aataaattaa atatactcta actaaattcc gtattatgaa
     4861 tatatcttct aattcagttt gttaattcat cgctccctct gctttgtaat ttccataaaa
     4921 accgttaaaa cggttttaac aaaaaactca cctccaccac ctcacgacat ccagttcctc
     4981 ttagcctcag cattccattt cgtagtagtc ttcttctgca atgtccaaaa ctctatcgaa
     5041 ctctttcccg tgatatcaca aactccaaat ttagactcat tccatccgcc aaatgaaaac
     5101 ggctctctcg gaacaggcac tccgatattt acacctatca tccctgcact tgctttgtcc
     5161 ataatatatc ttgcagctcc tccgctttgt gtgaatactg atgcggcgtt tccgtattcc
     5221 gaactgtttt cgatcttcag agcttcatca atatcgttaa ccctaatgat tgcaagtacc
     5281 gggccgaaga cttcttcttt ggcaattgcc atttcaggtt ttacaaaatc gatcactgta
     5341 ggtccgacat aaaatccttc ttctttacct ttgaccgaag ttttccttcc gtccactaaa
     5401 acttttgcac cttcattttc agcatcagta atatatcttt caattctttc tttcgcttct
     5461 ttacttatca ccgaaccgag attttctccg ggtataattt ttcttgcttc gtcacagatc
     5521 ttctcaacta tgctgtcgat ctcaccgaca cctaccattg cagatgcggc catgcatctc
     5581 tgaccggcgc agcctgacat tgatgcagct acgttgtttg cagtcatttc aggatgtgca
     5641 tccggtaata ctattaaatg atttttcgct ccgcctagtg ctatgcatct ttttagatta
     5701 gatgtagctc ttttgtaaac gatctttgct acttttgtcg atccgacaaa tgatactgct
     5761 tcaatccccg gatgatcaca gatcgcttct acaatttctc tgtctccgtt tacaacatta
     5821 aatacaccgt caggtagacc ggcttccttc agcatttcgg ctattcggtt tgcgctgatc
     5881 ggaaccagtt cagatggttt taatatcata cagttaccaa gtgcaattgc atttggtatt
     5941 gtccagtgag gtaccatgtg aggaaaattg aaaggtgcga tcgatgctac gactccgata
     6001 ggtttatatt caattcggca ttccactccc gaactcactt ccataatttc accgccgaca
     6061 agctgcggca gtgaacatgc aaattctgtc agctcaatac ttttttcaac ttcagcaatt
     6121 gattcgtcca ttgtttttcc attctcaatg tgggtaagct ctgcaagttc tttcagattt
     6181 ttttcaagca gggttttgta tctgtaaaat acctgtactc tttccttaat tggaattgct
     6241 gaccatcccg gaaatgcttc tttagctgaa tttactgctt cgtcaagatc tttcatagtt
     6301 gacataggta ctgtggaaat tattttaccg tctgaaggac agatcacttc cattgtcctg
     6361 tgtccgttct ttgttagttt tcccgatata taattcttaa cttcaggtat ctgctgattc
     6421 attttgtttt tatattattt gaaatattat cttttgtgaa aatagatttt tagcatttta
     6481 aaaaaaagga agattatgat tggcataagg aaatatcaat actatacaat taaaacttga
     6541 atattaatat ctctattaat atgttattaa aaatctattt atggcaaacg tttcaaaaca
     6601 atatttcata ttatttctat tccttttttt aatttctgtt ggaaatgttt tcccacagga
     6661 tatacctcca aaaatacttg ttgtaacagc tcacccggat gatgatgcac tttttgcagg
     6721 agctaattat aaaataattc atgatctgaa aggaacagtc gatcttgcct tgattacaaa
     6781 cggtgaaggc ggatataaat attcgacact cgcagaagat tattacggca tcgaacttac
     6841 aatagaagat accggcagga agtatcttcc cgagatcaga aaaaaagaac tcgaagccgg
     6901 cggagcgatc atcggcatca ggaattattt ttattttgat cagaaagaca accgttatat
     6961 cacaaccgaa aacattaggg aaattctcga cagcaatgta tgggatctgg attatatccg
     7021 atctaaatta aaggacatat taataaagga aaaatatgac tttgtattta ttatgactcc
     7081 gactgaagga acgcatgcac atcacagctc ttcagcaata cttgcacttc agacaattca
     7141 gtcacttgac gaaggtgcaa gaccaattgt acttgcaggt tcgggatctt cattgagcga
     7201 tacagttaag aatatttaca aaggtcttaa aggttttccg atcacaaatg taagcgatac
     7261 aattccgttt cagtttgacc ggacacagaa gttcggattc cgggaagttc tcgattataa
     7321 aataattgtc aactggctga tagccgaaca taagtctcag ggtacaatgc agcttggcat
     7381 gaaccgcggt gacattgaga attactggta tcttgacatt aatcctgaaa gcggaaaaat
     7441 aaaaactcag gagcttttcg ataaactaaa aataaattat tatccgaaaa aagaatatga
     7501 ttaatatatg aaaattttat ccccgctgtt aattctcatt tgtctgattt taataacagg
     7561 ctgcagtaaa gattcaagaa agaaacttac tgtttactct cctcatggaa aggaaatgct
     7621 gactgaattt gaacagcgtt ttgaggctgc aaatccggat acagatattc aatggctgga
     7681 tatgggttca caggaaattt ttgacaggat taaaacagaa aaagaaaatc cgatatgtga
     7741 tttatggtgg ggcgctcctg ctacgatttt tatgcgcgct gaaaaagaag gtttattaga
     7801 gaaatatatt cccacatggg ctgaaagtat tcccatatca agtaaaagta aagacggaaa
     7861 ctggttcggc acttatctta caccgcaggt aattgcattt aatgacaaaa ctcttacaaa
     7921 tgaaaaagct ccaaaagact gggaggatct cattaatcct gaatggaaag ataagatcat
     7981 tatcagaaat cctatggcat cagggacaat gcgtgtgatc ttctcggcag cgattgcaaa
     8041 ttccatcaag actaccggta atgataaaga aggatttgac tggctgagga aacttgatga
     8101 aaatacttct gactacgctg ctgatccgac acagatgtat ataaaacttt ccggggaaaa
     8161 ggaagctgtt actctctgga atatgcctga tataattctg cagaagactc tatacaatta
     8221 tccgtttgga tataatttcc cggttgatgg aacggtcgtt cttacggatg ccattgcaat
     8281 tattaatggt tcaaaaaatc ctgaaggtgc aaaagaattt tacgagtttg tcacaaatga
     8341 agaaagtctg ataattcagt cagaaaagtt tttcagaatc cctgcaagaa acgatattcc
     8401 aaaagataaa ctccctgaat gggtaagaca ggctgattat aaaaccatgg atcttgactg
     8461 ggaacttatc tctgagaatg aatccggctg gatgaaactc tgggatacag agattaaagg
     8521 aaagtcaaga taattttaaa atctgcaaat attaatttct gaaataccaa aatggcaaat
     8581 cttgagatca ggaatatcac aaaaaaattt aatgatacag aagttttatc tgacatgaac
     8641 attgatgtcc ggcagggaga atttttttca atagtcggac caagcggatg cggcaagact
     8701 acgctattga gaattatagg aggtcttgaa tttcccgatt ccggaaaaat cattgtcaac
     8761 ggaaaggaca ttacttctct tcctcctcag aaaagaaaga tcggcatcgt cttccagaat
     8821 tatgctctct ttccaaatat gaccgtctat caaaatatcg cctacggtct cgagatacta
     8881 aaaaccgata aatcggtttt aaataacaaa ataaactccg tccttgatat cgtctcgctc
     8941 tctcacaaaa caaataacag cgtcacttct ctcagcggcg gagaacagca gcgcgtatct
     9001 cttgcccgtg tcattgtcac cgaacccgaa ctcattctct ttgatgaacc gttatcaaat
     9061 ctcgactact ctttgcgtat cgaaacacgt aacgaactca aacgcctgca aagagatgtc
     9121 ggtattactt ctgtctatgt aactcacgat cagactgaag ctctcgccct ttctgacagg
     9181 atcgcagttt taaataaagg cgtagttcag cagactggtt cgccgtatga agtttattat
     9241 aatccgaaaa atagtttcac agcttcgttt atcggacacg ctaatttatt tgatgaaaaa
     9301 aattctgagc agatctttaa aataaataca ggtaaaaata aagtgctcgc acttctgcct
     9361 gaagaaataa tactgactaa agatgatgag gaaggcaaag gaaaaattac cgatgttcag
     9421 ttcacaggat tttcgattga atattctatt cagataatta attctgacag gataattaaa
     9481 actctttgta ctactacttc cgaaacagaa catttaaaaa caggtgacag ggtttcatta
     9541 aaaatttctg aaaataacag agatagtaat ttgagaatac ttgataagta atgaaaaaac
     9601 tttcatccgt aaataaattt ttactggcag ttataatttt ttttacattc ggatatataa
     9661 tttatccgat gatctcactt gtcagagaaa gttttacact ggcagacgga tcgtttacac
     9721 cgacagcttt tcttaatgca ttttcaccat cctctgtatc tgcaacttta aattcaattt
     9781 tactctcagt tataacagtc attggcagtg catttatcgg aattctgttt gcgtatatct
     9841 ttcaatatca ccgcattcct ttgaaatcat ttttctcatc aatcatcata attcctgttg
     9901 ctactccacc gctgatcggt gtagttgcat ttcttttttt actcagtgaa aatgggctaa
     9961 tctcaaagtt acttacattt gtattcggca tgaacggatc gctgttcagc tttgacggat
    10021 gggcggcgat cataacaata catgtatatt ctttctatcc tcttttctat ttatttgtat
    10081 cttcagcttt aaaaaagatc gacggtaata caattgatgc atcttattca ctgggagcat
    10141 caaagtcgaa aacttttttt aatgtgatcg ttcctcagct tatcccgtcc atgataggag
    10201 cttcgctcat cgtctttatg gcgagcatgg cgtcattctc agcgcctttt attttcggcg
    10261 ggtcggaaag atttttaact acggaaattt attctgcaaa aataaacgga gataattcag
    10321 ctgcatcctc acttgctgta atgctgacta taatttcttt actgttcctg ctgctgctca
    10381 gattttacag atcacggaaa acttatgaga tcagatataa aggaactccg aggactgtca
    10441 ttaccggaca taaaaaaggt atcaatattt taagctcaac atttactgtg atcttttctt
    10501 ttttaatagt tttgccgatt ttcactttgc tgttcctttc tttcataccc gaaggatctt
    10561 tgatgagaaa tttctttgct gagccgttca catttggaaa ttattcaaag atctttggtg
    10621 aagcacagtt ctttgatcct tttataaaca gtctgcagat gtccgtcatt gcagtagcaa
    10681 taactgttgt cattggagtc ggtacggctt ttctgatcgt taagaaaaat gtaaaaggag
    10741 gaaattatct cgagagtatt ttgtcgctgc catacggcat tccgggaact gtcatagcac
    10801 ttgcattcat tctctcattt aattttccaa gtgtatttac aggtttcatt gcactggtcg
    10861 gaacgttctg gatactgccg cttgcatacg ccgtcagaaa tctgccaata cttatgcagt
    10921 cttcaatatc agggtttcat tcgattgatc cgtctctgga ggaagcatct ttttctatgg
    10981 gagcaacagg tgtccgtaca ttcagaaaga tcacaggtcc attgatcttt cccgctgtgc
    11041 tcaacggagc actgcttgtg tttataaatt caatcggtga attcgtttct acaattcttc
    11101 tctatactta ttccacgaaa actatttcaa tagaaattta ttctcagctc agaatgtaca
    11161 acaccggtgc agcagcttct tacggcatca tattatttct tatcgttatg atagtggtat
    11221 atttttcgag aaagactctt gataaatctg tcggaataag ttgaataaaa aagttttcaa
    11281 agtgactcat gctttttgga ttttatagta taggagaatt tttaggataa ttaaaattga
    11341 gaaaaataaa attgtatttt gttttttttt tatgtaaatc tagagaaaaa ggagaaagta
    11401 atgaaaacaa tcttacgctt tatatttatt atttcagtac tgatattttc agtaaatgca
    11461 tccatagcat taaatggctc ctacactgtc ggagctggag gtgattttcc tacaattgac
    11521 agcgctgtat caaccgcaca ggcacaggga ttaggcggac cggttatatt caatattaaa
    11581 tccggaatct ataatgaaga aattatttta cagaatatat ttgggttgag ttttacaaat
    11641 aatttgacat ttcaatccga aacagggaat gcagcagatg taatcataaa tggaaatgtg
    11701 ttcgtgtttc aggtaaaagc tagttttgtg ttttttcaaa acctaactat cggcagctca
    11761 tcccaaacca gttttcagat ctattcatct tacactacaa ttaaaaataa tatctttaca
    11821 aatgattcga aaggaatttt aacttttgat cagtttcctc cgcgcaatat agaaataacg
    11881 ggaaatacaa acgtacccgg tataggtctc aatccgtgga gggattatca aggacaaaat
    11941 gtgattatta aaaataattc tgtaagtggg ggaattgttg tttattttta tttaaatatt
    12001 ttaatagaaa ataatattac gaaatttatc aataccagtt ccagttctaa tgcgactatt
    12061 tcaaaaaaca aagtgaatgg aacaattgaa tgtggcggag gatattataa tattttcaat
    12121 aattttttat ttagtgattc accaaacgac tctgctattt ttagtaacgc aataggaaat
    12181 gtcatttata acacattcag ttattcttcc cgaaccagta taattctctt tggtgacagt
    12241 acaacttttt tgaataattt aatgatcaat aaaagcggaa gatattcttt ttatgtgtat
    12301 tcaggtggtc ctttttttgg gaatcctaag tattcagatt ataacaattt ttataacaat
    12361 ggaaatacgg gattgataaa atggcataat gatgattttg ataatgtaag ttcatatttc
    12421 aacgctaccg gtaatgacga acattcaaac tcacatcccg tcacatttgt ttctccgaca
    12481 gatcttcatc tcgcaggttc ttcactggga gatcagcagc ttgccgggat ccctacttct
    12541 ttagtacttg atgatataga cggtcagccg cgtgacccta gccatcctta taaaggtgct
    12601 gatgaatata tagattttcc attgccggtt gagcttgctt cattcacttc atctgtctcc
    12661 aataacagag taaacttaaa atggacaact tcatctgaaa taaataattc aggatttgat
    12721 attgaaaggt cttttctcaa aaatagtata tcagaagatt ggttaaaggt cggttcgcag
    12781 acaggaaacg gcactacaaa ttatcaaagc agttatgaat tcaatgaaaa agatctttta
    12841 cccgggaaat attattacag attaaaacaa attgattaca atggaaactt tagatatcat
    12901 aatttaaccg gtgagattgt tattggtatt ccagataaat ttacactttc tcagaattac
    12961 cctaatccgt ttaatccaaa aactgttatt agtttcggac tgcagttagc cgggagaatt
    13021 tctataaaga tttatgatgt taatggaaaa gaaatttcat cattggtaaa tcaatcaatg
    13081 gaagcaggat ttcattctac agaatttgat ggttcaaatt tttccagcgg agtgtatttc
    13141 tacgtattaa attttgaagg taaaaacatt gatagaaaaa gaatggtctt gttgaaataa
    13201 atcaactatc agaaattcga agtcaatatg aaatgtttct gtacagtaac tttcaaaagg
    13261 tctagagtta tctataaaac tatctctgca aagtcactta aaaactgtcc ccgcagagtc
    13321 acatgctttt cagactctgc ggttttgtaa atagaaacat tcagttcaaa gtctccaaag
    13381 agtctcgtgt ttttaggatt ctgcggagta acgaatttaa aactcaaccc ggattaacaa
    13441 ttttttcata ataattactg tgaccactct cagctaattc ctttagtata aacagtgctt
    13501 tatcccatgc ataagtagac cattcctcat aacttttttc aagctcctga taaatactca
    13561 aaacagtagc accatctttt ccactaagac tgtaaatctc tttcatgcct ttccattttt
    13621 ccgcttcctc attgtcatag acctcttcat tgtttaataa tattccctga tgttccatag
    13681 aaataatttc gtttggtttg cactccatta atttacttac cagtccttca ccggttggag
    13741 ttttaaaaag tatcttgctg ccttctttcc agtctgtctg aaaatgagaa ccttcacaaa
    13801 actcagctgt ccaaatcctg tatggtttat cttcagtcaa aacttcccat atcttttctt
    13861 ttgttgcgtt gatcagaact tctttttcta ttactacttt tttcatattc tttgatttta
    13921 atccggggta atattttcag acactgtgga gtcctgattg ttaatttata gttataaata
    13981 gatctcgctt aattcagcgg tgtacttcaa taattttatt ttaaatggtt tcaatttttc
    14041 tgaatgcctt aagagttata tcctttctta tcccggcaaa ttcgtccata ttaaatccaa
    14101 taactggttc aacatttgtt gcaaaataga aaattccttt tttagtttca atatatccaa
    14161 caaaccaccc cagattattt tcaccactca cagcccagcc ggttttaccg ctgagtttgt
    14221 atatgtcgtt ctcatcaatt atcattatcc ttttcattgt ttcagatgta cgaactgaaa
    14281 tgggcagctc taaattataa agctttttca aaaaatctat ctgctgcatc tgtgagatct
    14341 ttgattcgcc tgtaagccag aatgtatcta tattgctact gtcaacaacc atatttccat
    14401 aatctaattt ttttaaataa tctttcattt taacagtacc gatctcccgt gcgatctgct
    14461 gatagcaagg tacacatgaa aactgaaatg cgtctttaag aatcagatcc ttttcccatt
    14521 gcggcagaaa ttttttcttg ccgtcccatt tgaaaactgt gctgtcatcc ttagctacac
    14581 ctgtctcaag agcaataatt gaatttggta ttttaaaagt agatgcagga ataaaaccgg
    14641 ttgagcatct tttgaaatca tttgaataat aaattttaga ctgcggatca aaaatcaaaa
    14701 cactaccgat caaacctaca ctgtcaatga tcatctgaaa gtcctcacgg tattcctctg
    14761 ttattttatc atgatgaata ttatcagatt cagaaaccag atctgaattt tcatttgaaa
    14821 tttgatcgga tttttttgga ggggaacagg atattaacag actgatactt agtataaata
    14881 aaatgatttt catttaatta atttgtggta aaattttatt taagaattgt tttggaatgc
    14941 aaccaattat tatcctaaag aatatttatg taaaataatt tattcaaaat atttttcaaa
    15001 ataataatca gcaatataat tttttaaaac gaataacttc gaatttcttt ttcagtttct
    15061 atgatctctc tgatctgttc gataaaccat ttatctattt ttgttgcctg aaatatttcc
    15121 tctgtcgttg catcgagtag taatgcatat ctgatataaa aaatattatc agatcgtgga
    15181 gtctgaagtt taataagtgt tttggatcgg agagaaattt tctcctcagt actgagattc
    15241 atgatctggt caaattcata tttatcttta ccatcagcac ccagtcctgc tcttcctgtt
    15301 tcaagagatc ggatagcttt ctgaaaagcc tctttaaaat ttctgccaaa agacatcact
    15361 tcacctacag atttcatttg tacaccgagc acgtcattat caggattggt ggatctgaat
    15421 ttatcaaaat cccatcttgg aattttaaca actacataat caatcaccgg ctcaaagcag
    15481 gaaggagttg tctttgtaat atcgtttgga atttcgtcaa gcgtataccc gacagccagc
    15541 ttagctgcaa tctttgcgat aggaaaaccg gtggcctttg atgcaagtgc tgaactcctt
    15601 gatacccgcg ggttcatttc aataacgatg accttgcctg tatccggatc tacagcaaac
    15661 tgaatattgg atccgcctgt ctccacaccg atctcggaaa taattttttt agcagcatct
    15721 ctaagttcct gatactgtct gtcactcaaa gtctgtgaag gtgcaactgt aattgaatca
    15781 cctgtgtgta ctcccatcgg atccatattt tcgattgtac atattacaac aaaattatca
    15841 ttcacatcac gcatgacttc aagttcaaat tctttccaac ctatcataga ttcctctact
    15901 aaaacttcat gagtcggaga agcatcgagt cctttattta caatcgtttc aaattcttct
    15961 acgttatagg caattcctcc tcctgttccg ccaagtgtaa atgaaggtct tatgatcaat
    16021 ggaaatccgg ttttatcggc aaactctttt gcatcttcaa aattttttat aaactttcca
    16081 ttggagacat tgagtccgat ctttttcatt gcgttatgaa agagttcgcg ggattcggct
    16141 ttatttatgg aatcaacttt tgcgccaata agctcaacat tatatttttg caatatacct
    16201 ttttcatgaa gatccattgc tgcattcagt gcggtctgcc ctcccattgt aggaaggatt
    16261 gcatcgggtt tttcttttct gatgatcatt tcaatatatt tactattgat cggttctata
    16321 taggttgcgt ctgcaatttc cggatcggtc atgatagtcg caggattgga attaatgagt
    16381 acgactctgt atccttcttc tttaagtgcc ttgaccgcct gcgtcccaga ataatcaaat
    16441 tcacaagcct gtcctatcac aataggaccg ctgccgatta tcagaattgt cttaatatca
    16501 gttctttttg gcatcggaat ttttattaaa atattttaaa gaattaaatc tgaatttcac
    16561 taaactgttt ccttcttttt atgaactccg ttatgtataa ctttttttcc tagagattct
    16621 ttcaattctt tctcagtttt tattactgct ttctttaata aaaattcctg aatgtttttt
    16681 ttctcaaact gaatatcatt cagtttaaga aaatcctctt tgcgtatata cgaaccgtca
    16741 tcgagcatga cccaggtctt tgcagtatct gtaagatata atttgagaat atcgatagcg
    16801 tcctgtttaa ttcttttgtc ttcgatggga aaaaatatct cgactcttct gtcaaaattt
    16861 ctttgcatta tatcagcact gccgaagtaa agttttggtt ctccattgtt atggaaataa
    16921 tatgcacggc tgtgctcgag aaatctccca actatactgt aaacgttaat gttttcgcta
    16981 agtccttcaa cctgtggacg aagtctgcat attcctctgg taatcaaatc gatctttaca
    17041 cctgcttttg aagcctgata aagttttaaa ataacttctt catcaacaac tgaattattt
    17101 ttaaataaaa tataaccgtt attattttct ttgtgcgatt ttatttcatc atcaataagt
    17161 ttcagtactt tccttcttag agtaataggt gcaacaagta atttattaaa cgacttttgt
    17221 ttggaataac cggtaaggta attaaaaaga tttgaagcat ctttgcaaaa atcctgatca
    17281 gtagtaaaaa gtccaaagtc tgtatatatc ctggatgtaa ccgagttata atttcctgta
    17341 cttaaatgca gatatctttt cattcctttt ttttctttcc ttacaacaag tgccattttg
    17401 cagtgagtct taagtccaac aagaccataa accacatgaa ctccggcatt ttctaattcc
    17461 cttgcccaaa taatattatt ctcttcatca aatctggctt tgagttcgac tacggcagta
    17521 acctgtatgc cgttttctgc agcttcaata agtgatttga taattaacga atcgccgctg
    17581 gtcctgtaaa gtgtaagctt gatagcatat acattagggt cttcagcaga ttgtgcaatg
    17641 aattcactaa cagatgaaaa ggaataaaaa ggatgatgaa gcataatatc attttctgaa
    17701 atagctttaa aaaaatcttc ttcctgtctg aaataagaag aaattcttgg tgtaaaacct
    17761 tcgtacttta attctcttat atcgatttta tatagtgaca ttaggtcacc gagattaagc
    17821 attccgtcta ttttataaat ttcattattc tcagccttta aaatattttt cagaaacgca
    17881 agaagtatat caggcatttt attatcaact tctagtctta ctaaaatccc cagtcttctt
    17941 ttttttactt cttcttcaat aagagtaagc aggtctgcag cttcctcttc ttcaagttcc
    18001 agatcagcat ttcgtgtaat acgaaaagca taggaatcaa ttactttcat tccgggaaac
    18061 agctttacag cattagcttt aattatatct tcaagtaaaa taaaatgata ttccgttcca
    18121 ttatttatga gataaaacct tgaaatatta tttggtatct gaattacaca tactttttcc
    18181 tcgacgactt cagtatcagg atcatccagt aatattgcaa gtgcaaggct tctgtttatt
    18241 aaattaggaa aagggtgtac gttatcaatt gcaagcggcg taagaacagg aaataactcc
    18301 tggtcaaaat atttgtctaa tactgatttt ttttcatcat taagtttttc ataattcaga
    18361 aattcaattt tatttgattt cagcagtggt ttaatctcat cattaaaaat cctgtgcagg
    18421 tcttcaattt gcggagtaag aacttcaaat actttatcct gctgttcctg tgctgtcatt
    18481 ccgtcagtag aaagatcatt cacctggctg gtgatctgcc tttttaatcc ggcaaatcta
    18541 atcataaaaa actcatcaag attagtactg aagatagaaa ggaatttaag tctttcaagc
    18601 agaggttgag aagaatcttt tgcttcttct aaaactcttt tattgaactc aatccagctt
    18661 aattccctgt ttaaaaaata ttctgatttt ttctttgaca tgtaaataaa tttaatttta
    18721 atagctaaga ttttccatgt aaattttttt attttatatg acttgtttac aatcataaac
    18781 attccgaatt tgtgaagccc cgatatttaa aaattaaacc tattttatgg ggaaaaatat
    18841 atttagctta gttaagttta caacatatct catctgtact ttcctgttat tttctttagc
    18901 ttcaaactcc aaagctgtta caattactga agatttagat cctgagacaa cactctggct
    18961 acagcgtgtt agtgctgcag gtgggtcagt ttctgacagc atagtttctg cagtagatga
    19021 ttatataaaa gaaataaaag gtttaaaata tcagagctta aatatcagag acaggatatt
    19081 tcgtgagaat tggttttgtg gaaattttaa tgctgctttt gtaccgatta ttgtaaatcc
    19141 aaccggttct cctttagcta tgggaaatcc aacggatgtg aacagaaatt ttacttcttc
    19201 aaattataaa gataccggta aatatagcgg attaaaaggg aatggagtaa accagtatat
    19261 tgaaacagga tttacacctt cactgataaa tgaattccct ttaaatgatg tttatttttt
    19321 attctattgc cttacagatt cagtagataa tggcagatcc ggcagcagag gaacttcggg
    19381 cagcggcttt tatgcttatc caaaatttat tacaggaaat tcacattttg tattaaattc
    19441 caatactggc ggggttgtag tatttcagtc aattaaaggt tacactcttc atcaaagatc
    19501 taacagccag gagattaaat cttactattc attgaatcag gttggtacat ctcaggtagt
    19561 cacgacatca aaaccggata aacaaatata tatatgtgct tttaataata acggagtaac
    19621 agacacatat tccacaacga gatacggcgg atattcattt ggtaaaagtt tttctgaaca
    19681 ggctagagtt gtacatttca atgctgttca aagactaatg tccagacttg gaaggaaatt
    19741 gcctgagtca attcagactg atttgaaatc aaaactcatg acatttaccg gtaaaaatct
    19801 tatagtaaat tatgaaactc gaaataacgg aaatattaaa tttggtctta gtgatatcaa
    19861 tggaaatgtg ataagtggtt attcagtaga taactgttta cctttaactg gaaatgaatt
    19921 tacaaaaaca gtttcctgga attcaggaaa tgatttaagt tcttttgtaa atactccatt
    19981 atatttaaat ataaggatga cagatgctga tttatatagt attcaatttc aaaatcaaat
    20041 cgtccagact gatagttcga agcagggatt caagataggt acttataaac aattctttgc
    20101 tgacacatta tttataggga gttttccaag tgttatgaga aagatgcata atcctgtaaa
    20161 agatcctcag cctgtagtga gacctgaaca agaatgggaa gaaaaccgtc tttttacaac
    20221 ttatagcaat gtaacatatt cgaaaagcat tgaacaaaac agaatggttt tcaaaatgtg
    20281 gctcagggct gtaaccggca catatagact acctgtatat tatgaatcag tagatggtgt
    20341 aaactggacc cggccggtgc ttgagcagtt taaatttaat aacagcatgg aaaataatat
    20401 catgtctgat gctccatatc ctggaggttt atacacagta gtagatgatt cactttataa
    20461 tcaatcagat tctaccagaa gatataaatc tatgttcaac acacactcaa atggtttgag
    20521 ttatcttaat gtttcatttt catatgacgg tataacctgg ataccatatg cagataatcc
    20581 tgtagggtat atgggagaag atctgacatc agggggctgg aatcctgtga ttggaaaata
    20641 cttaggatat ttcagagatt cactgggtat tagaaacgtt tcaagatacg taagtaatga
    20701 ctggattaac tggacgaaga caggtaaagt attagtgccg gatgcaaatg accaggcagt
    20761 taccggatat tataatttac atgtattatt caaggacagt gtttactggg gatttttagg
    20821 acatttacag cttaattcaa acggagatga aaatcctcca aatccatctc gaacggataa
    20881 tacagttcat attgaattgg tattttcaag agacggaata aatttcacta gatgcggaaa
    20941 tagaatgccg tttcttgaca taggtccact tgctgcatgg gatgatcaat gtgtttattc
    21001 tattggagtg ccaattgttg ttggtaatga atattattat tattataacg gatttaacac
    21061 aaagcatatg tggacagcac ccccacctgc aaacggtcaa ccaaagactg cacaggttgg
    21121 attggcaaaa ataggattag acagatttat ttctttgtcg acatattaat tatgaataca
    21181 tttaagtcat gagacataaa taatgagatc agaactttaa ttggatttaa cgaaatcaca
    21241 aagaaactga atatatcaga tatcacacag tcggaaagtc tcagttctta agacgctaaa
    21301 tgaaaaagat ttcagtttag aaaaggaagt taatgagaat ctgacaggat tgggatttca
    21361 tgaagcatat catttaggtc agattgggat attaaggaga atgaccggta gaaaaagtct
    21421 gataggataa tagagcaaaa accgtttaaa cggtttcctg ctctattaaa ctgagattgc
    21481 aattattcta acaagataaa tttctattga tatttactct gaaggaattt tcacttcttc
    21541 tattgactta ggttatttct gaaataaacc gttaaaacgg tttctacccc aaatcctcac
    21601 ctctcaacaa atttcaaaga aaccgaattt atacaatatc ttaatcccgt tggcgcaggt
    21661 ccgtcatcaa atacgtgacc caaatgtgct ccgcatcggc tgcataatac ttcatcccgc
    21721 gacattcctt ttgaattatc cacacctact attatattct ccttactaaa aggctcgtaa
    21781 aaactcggcc agccggttcc tgaatcaaat tttgtattcg attcaaataa ttcctgatcg
    21841 cacccaatac acaaatattt ccctgatctt ttatttttat catattctcc tgtaaatgca
    21901 cgttcagttc ctttttgtct tgtcactctg tactgttcct ctgttaatat tttttgccat
    21961 tcctcttcgg tctttactat ttcaaactca gaagtactct cctcttttac ctgagacttt
    22021 atcgaatcct gaaaattgcc aagagttact tcctcttttt tcgaacatga aaataatgtt
    22081 actaacatta aaaatattgt taacataatt ggttttataa tcatttttgg tttaaaaatt
    22141 tattgtgtgt gttttaacct ttatcttttc tgacaagttc atcctaaaaa aaaatatcag
    22201 acaagatatt cactatataa ctctaaaatt cttttatatt cttttaccgc ttcttctttg
    22261 ttgtatccct cctgctgtat agtaaaggaa agtgatctta attttcctgt gcattctaat
    22321 gattttgtaa gatgatcact tactgaaagt tgcttgtgca ttccagtaaa aatagttgct
    22381 gtcgctgtga atacagctac tattccgcat gtccatttcc aggcaacagc tccctgtcct
    22441 gcaacaggtc ctgtaaaagc tgttaatccg gcaagcagtg ttgccagtac acttgcaata
    22501 agacttatca ttataaatct ggaatttgtt ttctttagtt taatgtaatt tttctccgac
    22561 tttaaaagac tttccctaat ttcatttgtc attttatttc tcctgtgaat ttagttatta
    22621 acaaatataa taaaaataat tttttgaatt tttatttatt ttatattact caatatcttt
    22681 taaatcaatt gatttaattt catttattta catccatatg tttattcgga taaaaaacat
    22741 tatcgaaaat cttatataaa ttcaatattt tatatataca tttttaaatt taaattaata
    22801 taacatggca gcattaagat taggagacac agcacccgat tttacagcaa agactactga
    22861 aggagagatc atctttcata aatggcttgg agataaatgg ggagttcttt tttcgcatcc
    22921 cgctgatttc acacctgttt gtacaactga gcttggtatg gtatctaaac tcaaagatga
    22981 gtttgataaa aggaacgtaa aagcaatagg actgagcgta gatccgcttg actcacatat
    23041 taaatggatc aatgacatta atgaaactca gaatacaaca gttaactttc caatgatcgc
    23101 agatgaaaat aagtatgttg ctaatcttta cgatatgata catccgaatg ctgataatac
    23161 tttaactgtc agatctgttt ttattgtcgg acctgataaa aaaattaaac ttactcttac
    23221 ttatccggct tctacaggca gagatttttt tgaaatttta agagcaatag attctcttca
    23281 acttactgcg aattattcag tggctactcc ggttaactgg aaagacggcg atgattgcat
    23341 tatagttaat tcgatttcta atgaagatgc tgtaaataaa tttccaaaag gatttaaaga
    23401 aattaaacct tatatgagag ttacaccaca gccaaataag taatttacat atttgatatt
    23461 ataatgacag gcaaagtaag ttttttacat agtctgtctt aaattctttc atccgaaacc
    23521 taaagactta cttaccgttt tagatgaagt cttttattta atgttaaaag atttttaatc
    23581 tgtaaaagtt ttaacacctc actgcatttt tatgaataat taatttagtt aaattaatcc
    23641 agtacttttt aaatttatgt taagattttt taatctgaaa aatttctttc tgaataaatg
    23701 aaatatattt taatagcaat cttatgtgtc tccttcttaa atgacggaat agctcaaact
    23761 gatggtgtca gcaaaaattc ttctccattt aaaattgcaa gactgaaata ctctggcggt
    23821 ggtgactggt ataatgaccc gtctgctgag atcaatatga tggagtatct taaaaaaaat
    23881 actgtcattg atgttgatga accaaaattc tattctgtcg atgtcgcttc tgatgatata
    23941 tttaattatc cattcatttt aattacgggt cacggaaaca tagagctttc acaatctgaa
    24001 gctgcacgcc tccgaaagta tctggaatca ggcggttttt tatatgccga tgatgattat
    24061 ggaatgaatg agtcttttag aaaagaactt aagaaaatat ttccggatga tgaacttcag
    24121 gaacttccct tcagccacga aatatataat tcacattttt catttcccgg aggattgcct
    24181 aagattcatg aacatgacca aaaacctccg cagggattcg ggatttataa aaatggaaga
    24241 ctctgtgttt attatacctt tgaaacaaat atatctgacg gatgggcaga tacaaaagaa
    24301 catcaggatc cgcccgaaaa aaaggaagag tcttttaaaa tgggtactaa catcattgtg
    24361 tattctctga tgaattagtt ttatcaattt taatgctaat tttattttaa ttgtaaaatg
    24421 atcttaaaaa aatatcctga ttcgattcct aatgtcaaag ataatttcat agttcatttt
    24481 acttcacatt cttcagaact cgacagacat ataactcctc ttactgtcaa atgctctatg
    24541 aagggacttg agaagtttca tacttcagaa agttcatatg gtgtaagtcc cgcaagtttc
    24601 ctgatactga atgaaggtca gcaatgtgaa agctctataa taaacgaatc tgaatctttc
    24661 tcaatttact tcgaacccag gtttgcaaat tcagctttga aaagtcttat tactccaagt
    24721 gataaaatgc tgaatcattc atttaagccg gtaagtcagc cggtacattt ttttgaaaaa
    24781 ttgtatccgc ataataattt tctttcaccg gtaataatga aaatgagact ggcttcaaaa
    24841 gtaagttatg atgatgaaaa ctggctgaaa gaaagatatc tggaaatgct tgacggtctt
    24901 ctgaaagttc acaaagattt gtataaggaa attgaaaaac ttccaccggt aaaactttcc
    24961 actaaaactg aactatacag aagagtcagt attgcaaaag aatatattga tttggaattt
    25021 acaaatgatc ttacacttga taaaatttct aaaaaagcat gtctgtcaca atttcatttc
    25081 ctcagaattt ttaaatccgt ttacaaacaa actccgcatc agtatctcac taaaaaaaga
    25141 attgataaag caatagatct tttaagaaaa tctgacatgt ctgttacaga aatctgcttt
    25201 gagatcggct ttgaaagtgt tggttcattc agctggttat ttaaaaataa atttggatta
    25261 tctccagaga cattcaaaga aagctaccgc agaactatga tgaaatttaa acttttaaaa
    25321 tagctcactt ttattttata aaaaccctga aatatttttc cgattcaaaa tttaaaattt
    25381 caattacaca tcaatatcct ttttcaaaat tagcaataat cgagaagtat taatttttag
    25441 aattctatat ttttatataa attttacaaa cttaaataaa ggagatagaa atggcaaact
    25501 caattaactg gttcgagata cctgtgacgg atttcggcag agctaaaaag ttttacagtg
    25561 aaatatttgg aattgaaatg catgaagaaa tgatgggacc gcatcagatg ggattctttc
    25621 cgggtgaagg tgtaagcgga gcgatcatta aagcggatgg atataaacct tctatggacg
    25681 gggctcttgt ctatctcgcc ggaggagaag atctgacagg cgtaatgaat aaaatcgaaa
    25741 gtgccggcgg caaaatactt caaccaaaat ccaaagtaac tgatgaaata ggttacattg
    25801 cgatctttgc tgatactgag ggaaataaag tagcacttca ttctccaaaa taaatttaat
    25861 ttttgcaact gtattttttg aacccggctg ctgaccgggt ttttagttga atttgcaatt
    25921 ctgaaatttc taagtgtaaa ttaaaagatt ttaagtatgg taatgttttt gaataatgtt
    25981 taatttataa ttaaaaaatt ctaagaaata atttaaacaa atgagatcag cattttttat
    26041 tcttttgatt tctttagccg gattcttttc gctgaatgaa atcgcatttc cacaatcttc
    26101 caatgatctg aaatcaggac tcgatcctga aaatattaat aattccatag atccgaattc
    26161 tgattttttt gactttgcct gtagtaactg gttagtaaaa aatcctatac cggctgaaag
    26221 cagcagatgg ggcacatggg atgtaattat agaaaagaat aatgaagttt taagatctgt
    26281 tctcgaaaaa gcttcttcta attcatcagc cccaaaagga agtattgaac aaaaacttgg
    26341 cgatctttac tttacttcaa tggatacagt gaaaattgaa aaagaaggta ttgcacctct
    26401 tgcagattac cttgccatga ttgacggaat ttccaataag gatgaatttc agaaagtact
    26461 ggcatattta aaatcgatcg gtacaggcgg agtattcagt tttcgcgcag gacaggatga
    26521 taaaaacagt aatgatgtga tactgcaatt atttcaaggt ggtcttggac ttccgggtaa
    26581 agagtattat acaaaagatg atgaaaattc cataaagacc cgcgaaaaat atatggagtt
    26641 tatgaataca atttttgtat tgatgggaaa tgataattcc actgcgggaa atatttcacg
    26701 gagaattatg gatattgaaa aacgcatgtc agatgcttca atgacaagag tcgaaatgag
    26761 acctgctgaa gcaacatatc atcttatgac tttaaaggaa gtcagagatc ttatgcctga
    26821 ttataatttt gatgttttgt ttgcggaaat cggattagaa aataatagta aatttgaaaa
    26881 aggaataaac gtaggtcagc ccgaattttt taaagaaatt aacagaatgc tgactgatgt
    26941 aagtcttgat gattggaaaa attatttgaa atggaatctt gtaaggaaca atgctgacaa
    27001 gcttagtacg gattttgaga aggctaattt tgaattttat ggaaagactc tcagaggcag
    27061 tgaagtacag aagccgagat ggaaaaaaag tatcagcttt gtcgaatctg caatggggga
    27121 accgcttggt cagttgtttg tcgagattag atttaaaccc gaaacaaaaa caaaagcact
    27181 tgagatggta aataatatta aagaatcatt taaagacagg atcaatcaga atgaatggat
    27241 gagcgaagtt actaaaaaag aagctttaaa aaaattaagt acttttaatg taaagattgg
    27301 atatacagat gaatggaaag attattcggg acttgagatt gacagggttt catttttaga
    27361 taatatgatc agagcttctg aatacaggat taagcttaat cttgataaaa taggaaagcc
    27421 cgttgacaaa aatgaatggt ttatgaatcc gcagactgtg aatgcttatt acagcgcttc
    27481 taataatgag atcgtatttc cagcgggtgt aatgcagccg ccattttttg atccggaggt
    27541 tgaagatgct gtaaattacg gcgggatcgg tgcggtcatt ggacatgaga tctcacacgg
    27601 ttttgatgat cagggaagaa aatatgacgg agatggaaat cttaaagact ggtggaccga
    27661 agaagatgca gacaaattta aggtacgcgc agaaatgctg gcaaaacagt acagcggtta
    27721 tcaggctgtc gattcctatt atgtggatgg aaatttaact ttgggtgaaa acattgctga
    27781 ccttggcgga cttattacag cttatcatgc atacatgaaa actatagatt ataatgatga
    27841 tgttaaaatt gacgggttta caccgtcgca aagattcttt cttggatttg ctcaaatttg
    27901 gagaggtgat tacagacctg aaatgttgac tatgttagtt aaaacagatt cacattcacc
    27961 accaaaattc agagtaaatg gaacgatcta taacattccg gaatttatga aagcctttgg
    28021 tggaaaagaa ggtgatccga tgataaattc aaaagataaa attgtagtga tctggtaata
    28081 gaaataatat cttacattta ttttaaatat attttaaagt tagtgcgggt gtaaatttct
    28141 tacaggtcac attaaaatta aaaatttccg gattcagtaa atgtgtatcc ggaaattttt
    28201 acaaatcatc tcaaaaataa ttaatcagaa gaaattatat ttaaaaaatc agaaagctct
    28261 ttctgaaatg ttaacgtcct gtcttttgca taccaaagct gaatttcttt tgtagcctcg
    28321 tcatgagaat aatcttttct tgatttagct tccctcatta tctcttgtgc tggttcaggt
    28381 ctgggtcccc atttcccaat tacctcgagc gaatcttttt tgatcagaag aaatacagga
    28441 atagacctgg taccttttgt aagaaactga tccataacct caggattttc atctctgaaa
    28501 attattttta catccacatt tggattaaaa tcacttataa ctttcaaagg cggaagattc
    28561 tgtgctacat cgccgcacca tgcttcggca attaccagta cgattaaagg actattgatt
    28621 ttatcaagct gacgtttcaa ctcttcatta agtactgttg tcttttcaat ccgctttact
    28681 ctttcagcat taagcttcgt aaaatatata ttactttctg attgattatt gcctgaagtc
    28741 ttgtttttta aaattaattt tctaacaatc tctttatatg tatcataaga aaatgcgttt
    28801 tcaatcagct cttttgtaat aatgtttgac ataattatat taatttagat tgtatacaat
    28861 ttacgcaggt taaatcttaa tttatactca taaataaaaa aaaatccgga cagatcattc
    28921 cgtccggatt taatattata ttttagagct catctttaga ttataatcaa tgcatcacca
    28981 taactgtaaa atctatattt ctcttttata gcttttttgt atgccttcat aaccagatct
    29041 ctgtcagcaa tagaacaggt cagcatcaga agtgtactct gaggtaaatg gaaatttgtg
    29101 acgaatttat caattactct tactgactgt ggcggatgta tgaatttgtc tgtccagcct
    29161 tttccgtatc tgacatgctt actggtgact acattacttt ccagaactct tgcaacagat
    29221 gtaccgacag ctatgacttt tcttcctttt ttctgaatgg cagcattaat aatgtcagcc
    29281 gaatcttttg aaagctcata atattctgaa tccattctgt gctttgaaag atcttccact
    29341 tctacaagtc tgaatgttcc cagtccgata tgaagtgtga taaatgcaat tttaattcct
    29401 ttagcttcga tctttttcag aaggtctttt gtaaaatgca gacctgaagt aggagcagct
    29461 actgaaccaa tatttttcgc atatactgtt tgataatctt ctttgtcttt ttcagtggat
    29521 tctcttttga tgtaaggcgg aagcggtgtc ctgccgagct tttctatata ctttgaaaaa
    29581 tctccgttat aattaaatct tacgatcctt cctcttgaag tagtattatc tatcacttca
    29641 cagtaaagat tttttgtaaa aaatattctg ttaccaatcc ttacttttct ggccgggtct
    29701 acaactacgt cccagatatt ttcatcttca gataattgtc tcaatagaaa aacttcgatc
    29761 tttgcctttg tcttttcctt agtcccatat aatctcgcat tgaaaacctt tgagtcattt
    29821 agtacaagta cgtctccgga ttccatgtaa ttgattatgt ccttaaattt ctttgtttct
    29881 atatcacctg tttctttgtt gataaccatc agcttacagg catctcttgg tgactttgga
    29941 ttttttgcaa tcaggttttt tggaatcgcg tatttaaatt gtgaaagttt cattttttat
    30001 aagtgttata attgttctga ataatattat tttatattgt aaaaagcatt aatgcccgga
    30061 tagactgctg ttgaagacag ctcttcttcg attctcagaa gtctgttgta ttttgcaacc
    30121 ctgtctgttc gtgataaaga accggtcttg atctgacctg catttgtcgc aactgctaaa
    30181 tcagcaattg tagtatcctc tgtctcacca cttctgtgac tgatcactgc tgtgtaatta
    30241 tttatctttg ccagctcaat agcattcagc gtttcagtaa gtgtgcctat ttgatttact
    30301 tttactagaa tggaatttcc aattttcttt tcaattcctt tttgcaggat ttcagtgtta
    30361 gtaacaaaaa gatcatctcc tacgatctgt actttctttc caagagcatc tgtcaaaagc
    30421 ttccagcccg cccagtcatt ttcagccatt ccgtcttcaa tgcttatgat aggataatct
    30481 tttacaagct tttcataaag ctttaccatc tgctctgaag tcttatcagg aatatgtgat
    30541 ttataaaaat tatacttacc gtttttccac atctcactcg aagctacatc gagtccgaga
    30601 taaatatctt tccctgcttt gaatccggct ttactgatcg cttccataat gacctcgatc
    30661 gcttcttcat tggatttcag attcggagca aatccgcctt cgtcacctac gtttgtattg
    30721 taattttttt tgtgaagtac atttctcagt gaatgaaaaa cttccgtccc gtatctcagt
    30781 gcttcagaaa aactttttgc ccctgcggga atgatcatga actcctgaaa atcaactgta
    30841 ttgtcagcat gctttccgcc gtttataata ttcatcatcg gcaccggaag tgtctttgca
    30901 ttcactccac cgatatattt ataaagcggc atcttcagat aatttgcaga tgcatgtgct
    30961 gcagcaagtg aaacgcctag taaggcattg gctccaagct tagatttgtt tttagtatta
    31021 tcaagtttta taaggacttc gtcaatcaga ttctgatcaa gactgttcat cccgttaatt
    31081 tttttcgcaa tcgtattttt aatatttccg actgcttttg aaactccttt tccgaggtaa
    31141 cgatttttgt caccgtcacg aagttcaacg gcttctcttt cacctgtgga agccccggaa
    31201 ggtacagcag ctctgccgat tgtaccgtct tctaatatta cttcggcttc taatgtaggg
    31261 tttcctcttg aatctaatat ctctcttgcg tggactttat gtattacggg catgtactgt
    31321 taaggtggtt tttaaaaagt cgtttaaaat aaactatata tgctgtgaaa gcaattcaaa
    31381 aaccggtgta aatttactca tccgtttaaa tcgtttaatt acaaatttct agattcaata
    31441 ttttctactt acttttttta acgacaaaaa aagtaacaaa aaaagtcgcc cgctggtgat
    31501 aacttgccgg aaattcttt
//
LOCUS       DAORVQ010000015        31373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_462_length_31373_cov_5.937033, whole genome shotgun sequence.
ACCESSION   DAORVQ010000015 DAORVQ010000000
VERSION     DAORVQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 31373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31373
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_31373_cov_5.937033"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLX80_02495"
     CDS             complement(<1..537)
                     /locus_tag="PLX80_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQY19696.1"
                     /translation="MSKLNCILSIRKYLLLLFTIFFTSGFCYSQSKDLYNDYEVYDVH
                     YHLTNYIQEGITVQQQLENMGNVVGRCALFGIPLQQMWDYGNSGDEAPKYYLDTDAPL
                     YYYSFNDAYIAMQYLGLTDDQKKRFDPMITAFNPADMYAAAHIKRVLLTFPGVFKGIG
                     EFTIHKEFVSPKVAGKVAS"
     gene            complement(629..1957)
                     /locus_tag="PLX80_02500"
     CDS             complement(629..1957)
                     /locus_tag="PLX80_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19697.1"
                     /translation="MKKTLLTNFIYLFIVVIFFAILPGQANAQDVPAEIDTLISADTV
                     AGVSSVYTPGEGFDLVKSKQGSLNIGAYGLFRYINQLPVEQSYVDHLGNTRNIDTRSD
                     FQLHRVMIFFRGFLFDPKFNYNLTLWTVNATNQIAVIGNMTYKFAKQFNLNAGWGALP
                     GMRSNHYSHPFWLAHDRVMADEYFRPYFSQGIWANGEAIPKIHYTVMVGNTSSNLDVS
                     ASRLAARLSRNLAYAASLYWRPTTGEFGRFASFSDYDEHTKLATNFGGAFTHSRENRF
                     SANIEDSPSNTGVRLSDALNLFQVGALAKDVVVVEADYNMVSASAGFKYKGFFLQTEW
                     YSRTLNKFNTTGPVPLTAMLDQGFYVQASYMVIPKWLELYSVGSQIWGQFNNSNEILG
                     GVNVFPYGKWFMRLNAQVIVVNRSPVSSSFGYYIGGLKGTILSAAVSFYL"
     gene            complement(2006..3199)
                     /locus_tag="PLX80_02505"
     CDS             complement(2006..3199)
                     /locus_tag="PLX80_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HQY19698.1"
                     /translation="MKKTVLLFTLLVSIFSIQLKAQENTFEIYGFVMSDMGYDFKTVN
                     PDWYDVLRPTKLPSFEGEFAPDGKVFFGVRQTRFGVKSFTPTPLGDLKTTFEFNLFGT
                     GADAGQTTFRLRHAYGELGKFGAGQTWSAFMDSDVFPNIIEYNGPPGMVWYRNIQVRY
                     MPIQGVSRLTFALEKPGGSADDGEYSDRVELEGTRSQFYFPDLTGQYRYGDDWGYVQL
                     SGIVKSLRWVDPTEDTLSLDGSAVGWGGNLSSNINITENDKLILNLVYGEGIENYMND
                     APADIGLDTLFSGRTATRPIKRIALPVTGIVSFLNHSWSKYFTSSVGYSMINIDNSNA
                     QLPIAFKRGQYVIANLLYYPVPSFMAGVEYQWGERQNKGGFISTDTKIQFSVKANFSK
                     IFSFK"
     gene            3734..5212
                     /locus_tag="PLX80_02510"
     CDS             3734..5212
                     /locus_tag="PLX80_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19699.1"
                     /translation="MNIIIVIPSVLKTKMSVVRKKNIKFLTLYNSFTANEKIDFDKFI
                     GNGLNGNKRNYNKILSSLKTDEKGIIDFSQTINSRTRWNRLSELSILADKFLTLKSLE
                     YNNFENRYLLLKEYDKRNLTSAFEQKYKQFKNELSKEPVVNYDYNMVNQLDKIYLNHL
                     KSDSDSKKYPLKISESGYNRLGIFLIEILENLIEVWTIKNSSVLIPKTSNEDIYSILS
                     MEKILFLFSGFSKSPNKLYPVIKFLYHIYKSLNDLTNLEFYKEAKKIFFRDLKEISKE
                     KREQYFLYMLNYNIELKNLAISGAAEELFFLIDKKLKEGLSADFKIKNFPLNHFRDII
                     IISLSLKKYKWTENFIKNYGPLLPPDIRFDTVSIGEAMLLFEKNDFLMCKDLLMKIKR
                     KTPFSFVDVFVLKLKVLYELKNFDHCHSELKKFNTYLRKERITKNLLINYSIEFCKAY
                     SLLLKLNDGLVKNNLINLQYILSKKILIAKKWITLKMEELKY"
     gene            complement(5406..6431)
                     /locus_tag="PLX80_02515"
     CDS             complement(5406..6431)
                     /locus_tag="PLX80_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19700.1"
                     /translation="MITNSRISKKLFLFFFIVLCCNEIKASYTLKSQDLITSPYPDGK
                     NIVYISLILNNISEVNASSQLIKADIVMTARWFDPRLKHNKKGGRLEDPQKIWGPFLT
                     FSNRLNISKSFPEQVTIHEDGTVFYTQRIFGDFTQNLNFREFPFDTQNFVLRLIEISF
                     NSNEISLEPDPEIRSGVGKNISLSDWKIVNWELSKSEYSIIEDSQKLPSLVFNITAQR
                     ESGFYLLIFAIPLILIIMMSWIVFWLPPNLSASQISVATTSMLTLIAYRFIVTSYLPR
                     ISYLTRMDIFILGSSILIFMTLVLAASSSNLTQKGKGDLAKKYDKRFRWIFPLLYIIV
                     IIIALTV"
     gene            complement(6808..9240)
                     /locus_tag="PLX80_02520"
     CDS             complement(6808..9240)
                     /locus_tag="PLX80_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY19701.1"
                     /translation="MKNLFSTSMVFFTLISNLFLSGNADAQMFWNKASEFIASDSSHV
                     AVPDSPSLDLTGNFTLECWVNPDSSVHPFSQILIQKRTPSTSSTGYALYLQQGKVTIT
                     INNNIGLIGKTVIQNNKWTHIAGRFIVATGTFSVYINGVLDTSVAKPGNTPAVNNDSV
                     ITGIGRGIGGIFSGKIDNIRIWNRGLTAAEIFMYRYTTLGVNTGIYNGLVLSLPFQKE
                     ESTSPVFSVNDYSGAGNNGFARNLTSFDMSNELSQIVTYNESAEFDGNDDYLSASSNT
                     ALSPVQSVTLEAWIYPKSVSNSVIIHKGSDNGAVTNFRLGIENRKLYAGIKQNFNFIS
                     NDTIGVNKWSHVAFTYSGITGKYCFYLNGKMIIDSTNIIGLISSSSDSLYIGGSVSLP
                     DFNGFIDEVRITEDEKTNSEITENIFRSVDKSNESIYDDVVYNLDGLNLSNTGQGTKL
                     NFRNNAAFSHPAANTLKPVSPCLRSGANYFQGSFNLSKTDLRIPFSGDAGISEDEIEI
                     YENIPITDVNVFVAMNHENMNNIRILLTAPNNQYALLYNESYNAGVDENLITVFDDQG
                     ALPNSNTYTSFSPSIQGNVNLNSAFTGNTKGTWKLTIFDDTGTDTGRLYCWGIRFNNK
                     TASENILETSLHIEGFYNEAANNTIRDTITYNLRKFNSPYNIIESKKFYLPLNNLSYV
                     SFNNVVEAAPYYIEAVHRNSIGVWSASDVTFSPFSHLATHKFVSLQSSAYGNNQIKID
                     TSPLRFGLYSGDVNQDGVIDIADGSLIDNDSFNFATGYIPSDTNGDNVTDLSDGVYAD
                     NNGFNFITKITP"
     gene            9510..10958
                     /locus_tag="PLX80_02525"
     CDS             9510..10958
                     /locus_tag="PLX80_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19702.1"
                     /translation="MKDSKLINLIETFTKTEFREFGMFVNSPYHNREKVMINFYNYLK
                     RIYPVFSEKNLERRIVFSKIFSEKKFSETLFRNTVSDLLKLTENFVKLKHFENEKFYG
                     QYLLLKELTNRKQISLFKSNFKRADKILNADNARDEIYFQNKFLLEDEWRRNHVVSSS
                     KMLFKEDNLKNQSDSLHVYYLTEFTKLYAILLNQSKFTYDYNFNYELFDAVIEYLRNN
                     FTLYKEIPYINIFYNCVMLYKTDERIYFDELKKLLLRHYRILTLTDRKNIFVVLINYS
                     SDQISKGNYSFIKEKFNINEHLLKSGAYFEGNDFMPHYFYESIAVNALEMKRFDWAEK
                     FILKYKKIVHPDYKESSFNFCMAELFYRKGDTDNSLSFLSKVLPENVKDRTKMNILLL
                     KIHYYCNNTVQFNSLIESSKKYILRNKNLQEDNKKQLANFLSLLVKLYKIKNDSDHLH
                     ELENLKKKIYDCTEFTEKKWLEEQVELIRLEN"
     gene            11007..12257
                     /locus_tag="PLX80_02530"
     CDS             11007..12257
                     /locus_tag="PLX80_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQY19703.1"
                     /translation="MNNILRIIFLIIVLTPLSENKEIYSRNSNDINAFISGRVVDERR
                     FPVPKVKVNIGNAEVTTDNSGKFSIPNISFPYNVTIAEKTSATAVIYKNLAINNPELV
                     LFGDLENKFSNYVALNISFPKIPDGGSAVVKLISTETFECKEATAVAGDSTIKIHVVW
                     PVSENYINGQVVYLLKDEKGFQTLKFTKVSIHRNDVDKKVTVSKKKGNALITSFVNVY
                     QPTEKNLKTEFDVSLFFFNYNLNSGIKFSENSSGNEKTQVWVPDKVSITNKILLRGSG
                     LYNNGSGYTGLNYVSPGADLKLYPESPPELFTPSDNYLGANGKTNFSYSLGSGTGIYV
                     IEFRSENPLLNIYVVNSDRKTNLSYLSRNEFYNGSYQFTWRVRKYITFFNESEFVKPN
                     VFKNDFSYKAVLYSEKRKFKTGYY"
     gene            complement(12412..14241)
                     /locus_tag="PLX80_02535"
     CDS             complement(12412..14241)
                     /locus_tag="PLX80_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY19704.1"
                     /translation="MNFLKTFLKPSTVLLILIVLFLFAKGSFAQNFVKVTDPNNPVVT
                     EALSGNYFGTSWVDIDNDRLLDLFINRKVIFRNLGNGNFVKIETAMANQNTNIGNSWS
                     DYNNDGLLDCFVTSTGGPFSYLFKNEGNGVINKVQAGDISDSLNNTGWGCAWADYNND
                     GFTDLFIAAANGFGNVFHPNRFFKNNGDGSFTKLEVAGLTDTLAPFTIPIWSDYDQDG
                     DMDLFIGSGPGGSTSTDFLYKNMLTETGTSDLVRLMTSPMATDQLDGQVWNWIDYDND
                     GDLDGFITNYANNVPNNLYRNNNGVYEKMLAAQVGNIVSDIGYYLANVWGDFDNDGDL
                     DCFLTRDVGQQSRYYNNNGNGTFTRMDSLQVVTLSGGNYGATSGDYDNNGTLDLFVTG
                     STNSKGLFKNELSNGNKWVNIKCIGSGPSNNLSNAAAIGTRVKAKATINGIEVWQQRE
                     VSAQNSFNSMNMLNVHMGFGNAAIIDSLIIIWPRGLKEVYTKVGLDEFYNAAEGQGIV
                     SGIVLESQILPEGFLLEQNYPNPFNPSTKIKFSIPSENKNLLFTLKIFDILGKEVQTL
                     FTKKISPGSYSYEFNGNGLSSGVYFYRLTSIGFSQTRKMVLAK"
     gene            14537..14998
                     /gene="rplM"
                     /locus_tag="PLX80_02540"
     CDS             14537..14998
                     /gene="rplM"
                     /locus_tag="PLX80_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQY19705.1"
                     /translation="MKSGIRKTNRITKFATKSENDHKWYVVDAEGKILGRLASEVAKR
                     IRGKYNPQYTPNADIGDWVVVLNADKIKIAAKRAEQKVYFTHSLYPGGQKKRTFNEMM
                     QKHPDRVIRLAVKRMLPKTKLGNKLINKLKVYAGTEHPHSAQNPEVLEIRS"
     gene            15044..15436
                     /gene="rpsI"
                     /locus_tag="PLX80_02545"
     CDS             15044..15436
                     /gene="rpsI"
                     /locus_tag="PLX80_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQY19706.1"
                     /translation="MSSNSTLKIGRRKTSTARVILTPGSGVISINKLEANKYFKTENL
                     YTEILQPFEVTGTTGTFDAQVNVNGGGFTGQAGAIKLGIARALVETNGELKSPLRKAG
                     LMTRDPRAVERKKCGQPKARKKFQFSKR"
     gene            15736..16476
                     /gene="rpsB"
                     /locus_tag="PLX80_02550"
     CDS             15736..16476
                     /gene="rpsB"
                     /locus_tag="PLX80_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQY19707.1"
                     /translation="MTQVQIEDLLLAGSHFGHLTRRWDPKMKKYIFMERNGIHIIDLK
                     KTLELLIEACNSISKISTDGKKVLFIGTKKQAKQIIKDEAERSGSFYVCERWLGGMLT
                     NFNTVRKSIKKLTNIQKMETDGTIDQFVKKERLILSRDKEKLEKVLNGIVSMTKLPGA
                     IFVVDIKKEHIAISEARKLNIPVYAIVDTNCDPDLVDYPIPANDDAVKSIQIITKAIA
                     DAAIVGTQVAKIKAEDESEEMKEKLEKR"
     gene            16507..17370
                     /gene="tsf"
                     /locus_tag="PLX80_02555"
     CDS             16507..17370
                     /gene="tsf"
                     /locus_tag="PLX80_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQY19708.1"
                     /translation="MEITLDLIKELRNKTGAGINDCKKALEEAEGEISKAIELLRKKG
                     AAMAVKRADKITNEGAVKLKISDDHKTGAIIEVNCETDFVSKGDEFQNFAESIAAATL
                     SVNSEDLEVILSSKTSEGLTLKDKLSEIMGKVGERTEFKRAKIINVPEGFVTGYIHFG
                     SKLGSLIGLKGTYNEDADKLGKNIAMQIVAMNPMAVNREDIDKDLIEKEREIYLSQAK
                     NENKPDNIAQTIVNNKIERYFQENCLLEQEYINESGKSIKNLIAEFNKANDNSLEIVS
                     IVRFQLGEETK"
     gene            17386..18096
                     /gene="pyrH"
                     /locus_tag="PLX80_02560"
     CDS             17386..18096
                     /gene="pyrH"
                     /locus_tag="PLX80_02560"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQY19709.1"
                     /translation="MTPKYTRILLKLSGESLMGRHSYGIDSDVLEAIAEEVLSISKLK
                     IEIGIVIGGGNIFRGLSAHEQGVDRETGDQMGMLATVINALAIQNALEKKGIFTRVQS
                     AIKMDEIAEPFILRRAVRHIEKKRVVIFSAGTGSPYFTTDTAAALRAIEIKADVIIKG
                     TRVNGVYDSDPEKNDKAVMYKKLSYLEVLEKDLRIMDLTAITLCQENSLPIKVFNMNI
                     KGNLKKLVMGKDIGTLVC"
     gene            18135..18692
                     /gene="frr"
                     /locus_tag="PLX80_02565"
     CDS             18135..18692
                     /gene="frr"
                     /locus_tag="PLX80_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQY19710.1"
                     /translation="MIKDILKNTSDLMDKAVEHVRLEMVKIRTGKATTNLLDGIKVDY
                     YGTPTALNQLGNVSTPDYHTITVQAWDKTAISLIEKAIIAANLGLNPANDGNLIRIPI
                     PALNEERRKEIVKLVKKFGEEGKISIRNIRRDGIEELKKAEKEDHFSEDERKHGENEI
                     QKLTDSFTKDIDNLLVVKEKEIMEV"
     gene            18705..19985
                     /locus_tag="PLX80_02570"
     CDS             18705..19985
                     /locus_tag="PLX80_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQY19711.1"
                     /translation="MKKFWFIVYNLFGIPLIWLFFKLYSVFNSKVRQGLKERRGLFND
                     LSRSLSHLKNNKTVIIHSSSLGEYQQAIPLADEFTKKNYNVVISFFSPSGFNNAKIIS
                     DNVIKTYIPLDSISNQKKFLDLINPNILIFMRYDLWYNILNESGKRGIKRVLANARYD
                     ENDSTWSIPVISSFKKNLYGMIDTAFVIDDFDEKNYSEKLKDENTKIVKIGDSKFERV
                     LQSVKINSNTEDVIPPSVINGRKVFVMGSAWKDDEDIILPALDKSLSFEKDLLTVLVP
                     HEPKETKISAIEKIISQKYENLRYIRLSDIGKFNGENLIIVDRIGILSKLYGIAYLSY
                     VGGGFKTGLHNILEPAIFNMPILFSNKIKNSDEDEILIEKGCGIVIKDTKQFYRVFRE
                     FLTNKELRDETGEKCRLVFENSSGIAEKIVNQIT"
     gene            19992..20582
                     /locus_tag="PLX80_02575"
     CDS             19992..20582
                     /locus_tag="PLX80_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQY19712.1"
                     /translation="MFNRKEFITTSLSESAEVKNMIAEKCYETICDASDLLVKSVKDG
                     KKILLCGNGGSAADCQHLATEFMIRLSHDLDRPAIAAIALTTDTSNLTAGGNDIGFEN
                     VFARNIEGLGNDGDVLICISTSGNSPNILKAVSKAKEKNMKVIGLLGKNNGGKLKEVC
                     DISIVIPSENTQRIQEGHITVGHILCEVTERELYEK"
     gene            complement(20895..22643)
                     /locus_tag="PLX80_02580"
     CDS             complement(20895..22643)
                     /locus_tag="PLX80_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY19713.1"
                     /translation="MNKILLIILLTFSSVNIFSQETADTKYWIIFNDKGDFKSDIVIT
                     PGSKAYDAGMELLTERAVQRRLKVLSEENLIDYADLPVENSYIQEIKNNGIEVIAVSR
                     WLNGISAYMTEAEVEKIKGLDFVDHLGYVKTLVNQEFEVVNEYIESEISDSSYKLDYG
                     KSLKQMEMVNVPKVHDMYINGKGVMIASFDDGFDWKNHESLMSLNIIEEYDFVNKDPN
                     TFAEKNQKYEDSKNQGSHGTSTVSTMSGFKEGRLIGPAFGSDLLLAKTEYVESETPME
                     EDFWLEATEWAEAKGVDIITSSLIYKTYDAPYNKNSYTYEELDGNTAIITNAADRAAY
                     LGVVVCQSMGNYFQTKEPSLGSAADADSIISIGAVTYSGDPASFTSNGPTSDGRIKPD
                     VVAPGVNVYSAKVKEISGNNSTYEFVSGTSFSTPITAGICALILSAHPELTPIQVRDA
                     LRNTASQSDNPDNVLGWGNVNAYDALLYFGMAWSNEFQIEKPESDKIIISTYLASKDI
                     IDPNSVTMHYSFDNGKSYSSMTMDLSEVMDAETNSGKYSAQLNLDKNMDDFKVYFSAE
                     DNSGKRSEFPKVSSAN"
     gene            complement(22715..24100)
                     /locus_tag="PLX80_02585"
     CDS             complement(22715..24100)
                     /locus_tag="PLX80_02585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQY19714.1"
                     /translation="MSVDIVMPKMGESIMEGTILKWHKKPGDNVDVDETILEISTDKV
                     DTEVPSPEAGVVSEILFKEGDVVEVGKVIARLGSNGAATSESEKAETKSSETSEVKEE
                     KSEPVPEMKSEKPEAASPQTNETQKIITGSGSKFYSPLVLNIAQVEGIGMSELENISG
                     TGIDGRVSKKDILAYLKDRTSAPAAESPKKSVEINLDNKDTKETKPAAAPERKIEVPQ
                     PKIDYNEEGLSVMEFDNVRQKMAEHMINSVRISPHVQAVAECDLTNVDKVRAKMKNEF
                     VEKEGFNLTYMPFICEAVIKALKDFPLINSIIDDSSVPYKAITRNSINLGIAVAMDNG
                     GLIVPVIHNSDGMNLIGIARSLNDLAKRARIKKLTLNEIQGGTFSITNFGVFGNDIGI
                     PIINQPQVAILGLGAIKKRPVVVETEEGDFIVPRKTMFITLSFDHRLVDGALGGMFVM
                     KIAHYLNSYKL"
     gene            complement(24185..25129)
                     /locus_tag="PLX80_02590"
     CDS             complement(24185..25129)
                     /locus_tag="PLX80_02590"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQY19715.1"
                     /translation="MMSDLKIFAGRFSNNLGKKIAASLKMPLGKCSIRTFSDGEIWVK
                     YNENIRGSDVFIVQSTMPPADNLMELLIMIDAAKRASARRVNAVIPYFGYARQDRKDQ
                     PRVSITAKLVANLITKAGADRVITMDLHAAQLQGFFDIPVDHLYGSAVFMNKLRKLKI
                     PNLAIVSPDVGGIKMARAYAKRLHADLIVIDKRRPKPNVAEVMDIIGDVGGKNIIIID
                     DLIDTGGTFVNAVNALKAEGAKDIYGGCTHAVFSGESLDHINKSPLKKLYVADTLPMS
                     HTSKKIEVVSVAEIFGKSIYRTNKNQSISSLFDVDKAL"
     gene            25330..26565
                     /locus_tag="PLX80_02595"
     CDS             25330..26565
                     /locus_tag="PLX80_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19716.1"
                     /translation="MKKIIPLFSLFFLLSFANQLKSQIPQYTLEAKNYVLSDEYTMKF
                     DLIFTKTDDTPLQLAGWQFFFRVPQTLAVFSAGFGSASAYMLDTSNGVPVSDLPESFR
                     PRGSNVVVAANSPGNYEFRLAANSLPGCGNGLIIPKDIPILIGRYNMKASTIINPNVL
                     LNNPIIFRDSCESPLSVTRTKINWYDANNCLNKEMTRCANHNVDISGVFPFIQINLKL
                     SIEGLYNPVFNRLNRKDSVTAYLRNVNSPYQIIDSSKGLIDSSTLSGNYIFYNRPAGT
                     YYISVKNKNGLETWSEQSGTNFQNGTNFYDFTISASKAYGNNLVLVGDKYCIYSGNFN
                     YDNIIDTDDLTMLDNDLYNYAGGNSLTNINGDDIVDIDDLAIVDKNAGNFVLVKWPGQ
                     TFQKRKKLLNNKTIARRTK"
     gene            26562..27671
                     /locus_tag="PLX80_02600"
     CDS             26562..27671
                     /locus_tag="PLX80_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19717.1"
                     /translation="MNILSKLFIFLYLILPVVSFSQAPLYNLTAKNFSHPAPNVLQFD
                     IFIEHTNAPTTFEYSGGQYFFNFNPLIANGGTLSYTYAGDTSELPQHMRPRNPQISGN
                     QLRLASNLFPGAGNGFIMTNNGNPGTRIVSMKLTTTAPSLTNAPLSFEWRNNLPDPYT
                     KIYAYVGNTNTNITSNAVHSIDSSGIILTFNLKVAIDGLLKTAFHVHNRRDTVTVYLA
                     KSVAPYNYVDSATNIIDSLSLSATFTYRNAVNGTYYLVVKHYNSLETWSKAGGETVMN
                     GNIYNYDFTTSASKAYGNNLALRGTLYCIYSGDVNQDYIIDISDLSDVDNDVFTYASG
                     FRPTDFNGDFVVEGRDMSIIDNNRNKLIRNPIYGD"
     gene            27703..28938
                     /locus_tag="PLX80_02605"
     CDS             27703..28938
                     /locus_tag="PLX80_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19718.1"
                     /translation="MKKNLLLFSIFCMLVLTNSSNAQLPQYTLEAKNFELTNAHTLKF
                     DLVFTNLDFIQWQLAGWQFFLKAPQTLGNFLPGFGTSSSFVLDTINGIPVSDIPESFR
                     PRNPNTVIASNSPGNYEFRIAANSLPGCGNGLSIPVNVPTLIGRYKLKSTSIIDISVL
                     NENSFTFRDSCDTPLSVTRTKINPYILSHCLGVEMTRCASHSVDLSGLENNISLNIIM
                     ANEGLYNPINNTLNRKESVTAFLRSVNTPYQILDSSVAFIDSVNLTANFIFENHLNGN
                     YYIAVKSRNTIETWSKSGGELLIVGGNSYNFTTSASQAFGNNMVLNGSRYCIYSGNVN
                     NDQIIDTDDLSMIDNDVYNYVLGNSITNLNGDNIVDIDDMAIVNRNAGQLILVEWPGQ
                     TTNKRNISLKSKTFTGDFK"
     gene            28989..30038
                     /locus_tag="PLX80_02610"
     CDS             28989..30038
                     /locus_tag="PLX80_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19719.1"
                     /translation="MFSQTPTFNLKSLNFVFTSPNVLEFEIRLEHTNSPTVFEYSGGQ
                     YFFNVNTDIANGGTLSYTYSSDSSDLPVNMRPRNPQVSGSQLRLAANIFPSAGNGFIM
                     TNNGSPGTKIAKMKIQTTAGSFANVPLNFAWRTQSPDPFTKIYAYVGTVNTNVTSGAS
                     YLIDTSGIQTSLEIKVAIEGLYRPIQNKHLKRDSIKLFLRKSVSPYQIIDSSISILDS
                     NTLLSNFTFRFIQGGSYYLVMKYINSLETWSRSGGENIVIGNTLNYDFTVASSKAYGN
                     NLVLKGSKYCLYSGDIDNSGFVDISDMSRVYNDGTNFLSGDVITDLNGDRFVDLDDLA
                     IADNNVFLFAGVITP"
     gene            30085..31335
                     /locus_tag="PLX80_02615"
     CDS             30085..31335
                     /locus_tag="PLX80_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19720.1"
                     /translation="MKKIIFILILISQLIIINESSAQIPQYTLEAKNFNLVSPYLLKF
                     DLVFTHTDATVMQLAGWTFFLRGPQSLGTFYAGFGSASSFMLDTANGVPVSDLPESFR
                     PRNTNTIIASNSPGNYEFRAAANSLPGCGGGLIMQQGVPILIGRYNLKSSTPINLSAL
                     DTNPIIFRDSCESPLSATRTKINWYDANGCLNKEMTRCSNHFINMNPVFVNVNLNLKI
                     SIEGLYNVSNDKLFKKETATVYLRKSTSPYQVVDSASAIIDSVNLTGNFDFQNISLFS
                     NYYIVVKTKNGLETWSKSGGVTLAEGNNFYDFTSSVSQAYGSNMVLKGSRGCIYSGNV
                     NNDQIIDSDDLVMIDNDLFNYTLGNAVTNLNGDIIVDIDDLAIADRNAAQLILVNWPG
                     LAFDKRKELEKKSIFKNQKIFVKN"
BASE COUNT        10762 a         5247 c         5320 g        10044 t
ORIGIN      
        1 actcgctacc ttaccggcaa ctttaggtga aacaaattct ttatggatcg tgaactcacc
       61 tattcctttg aacacaccag ggaaagtcag cagcactctt ttaatgtgag ccgctgcgta
      121 catgtcagca ggattgaatg ccgtgatcat cgggtcaaag cgctttttct ggtcatccgt
      181 caggccaaga tactgcatag caatataggc gtcattgaat gaataataat acaaaggcgc
      241 gtctgtatcg agataatact taggagcttc atcaccggaa tttccataat cccacatctg
      301 ctgaagaggt ataccgaaca gagcacatct tcctactacg ttacccatgt tttctaactg
      361 ctgctgaaca gtaataccct cctggatata attggtaaga tgataatgaa catcataaac
      421 ttcgtagtca ttataaaggt ctttgctctg agagtaacaa aatcccgagg taaaaaatat
      481 tgtaaacaag agcagtaaat atttccttat actaaggatg caattaagtt ttgacataag
      541 tgttaaaatt taaagttaaa attaaaaagt agtttttttt aaagttataa tatctgatta
      601 cattgttaaa tttataatac aaatggtttt ataaataaaa tgaaaccgct gcagataata
      661 ttgttccctt taatccaccg atataatatc cgaaagaact gctgaccggc gagcggttaa
      721 ctacgatcac ctgtgcatta agccgcataa accatttacc gtaagggaat acatttactc
      781 ctcccagtat ttcattcgaa ttgttaaatt gtccccagat ctgtgagccg acagaataca
      841 attcaagcca ctttggtatt accatgtaag atgcctgtac ataaaatccc tgatcaagca
      901 ttgcagttaa cggaaccggt cctgttgtat tgaatttatt taaagtacgg gaataccatt
      961 cagtctgaag aaaaaatcct ttgtatttga aacctgcaga tgcagaaacc atattgtaat
     1021 cagcttccac aactacaaca tcttttgcca gtgcaccgac ctgaaaaaga ttcaatgcat
     1081 ccgaaagacg tacgcccgta ttacttggag aatcttctat gtttgcgctg aaccggtttt
     1141 cacgagaatg agtaaaagct ccgccaaagt tagtcgccag tttcgtatgt tcatcataat
     1201 ctgaaaatga agcaaaccgg ccaaattccc ctgttgtcgg tctccagtaa agacttgcag
     1261 catatgcaag atttcgtgac agtcttgctg ccagccttga tgcactgaca tcaagattgc
     1321 tgcttgtatt tccaaccatt accgtataat ggatttttgg tattgcttcg ccatttgccc
     1381 agatcccctg agaaaaataa ggccggaaat attcatccgc cataactctg tcgtgtgcca
     1441 gccagaaagg atgtgagtaa tgatttgaac gcattcccgg taaagctccc cagccggcat
     1501 tcagattaaa ttgttttgca aatttatatg tcatgtttcc gattacagcg atctggtttg
     1561 tagcgttgac agtccagaga gttaaattgt aattaaactt cggatcaaac agaaatccgc
     1621 gaaagaaaat cattaccctg tgaagctgaa aatcacttcg tgtatctata ttccgtgtgt
     1681 ttccaagatg atcaacatag gattgttcta caggaagctg attgatatac ctgaataaac
     1741 catatgcgcc aatgtttaaa gaaccttgct tgctttttac taaatcaaat ccttctcccg
     1801 gagtatatac actgctcaca cctgccactg tatcagcact tataagagta tcaatttctg
     1861 caggaacatc ctgggcattg gcctgtccgg gaagtatggc aaagaaaatt actacaatga
     1921 acaagtagat gaaattagtc agtaacgttt ttttcattat gtatttttaa tttaagttat
     1981 aatttttgtg aattatttta tttcgttact taaaactgaa tatttttgaa aagttagcct
     2041 tgacggaaaa ctgaatcttt gtatcagtag agatgaagcc ccccttattt tgtctttctc
     2101 cccattgata ttcaacacct gccataaaac taggtaccgg ataataaagc agatttgcaa
     2161 ttacatactg tccacgtttg aaggcaattg gcaactgagc atttgaatta tcaatgttaa
     2221 tcattgaata tcctaccgaa cttgtaaaat atttactcca ggaatgatta agaaaggaaa
     2281 ctattccggt aacgggtaag gcaattcgtt ttattggtct tgttgcagta cgtccggaaa
     2341 ataaagtatc aagaccaata tcagcaggag catcattcat atagttttca attccttctc
     2401 cgtacactaa atttaatatc agtttgtcat tttccgttat atttatattg gaacttaaat
     2461 ttcctcccca gcccactgcg ctgccgtcaa gagataatgt atcctcagtt ggatcaaccc
     2521 atcgtaaact ttttactatt cctgatagct gaacatatcc ccagtcatca ccataccgat
     2581 attgccctgt aagatcagga aaataaaatt gtgaccttgt cccctcgagt tcaaccctat
     2641 cggaatactc accatcatca gcgcttcctc ccggtttttc aagagcaaat gttaatcttg
     2701 aaactccctg aataggcata tatcttactt gtatgttcct ataccaaacc atgcccggag
     2761 gaccgttgta ttcaattatg ttcggaaata cgtccgagtc cataaatgcg ctccatgttt
     2821 ggccggctcc aaattttcca agttctccat aagcatgccg cagtctgaaa gtagtctgac
     2881 cggcatctgc acctgttccg aaaagattaa attcaaatgt tgtttttagg tcacccaggg
     2941 gtgtgggggt aaaggatttt acaccaaaac gtgtttgtct cactccaaaa aaaacttttc
     3001 catcaggggc aaattctcct tcaaaggaag gaagctttgt aggtctgagt acatcatacc
     3061 agtccggatt taccgttttg aaatcataac ccatatcgga cattacaaaa ccgtaaattt
     3121 caaaagtatt ctcctgcgct tttaattgaa ttgaaaaaat actgacaagc aaagtaaata
     3181 atagtacagt ttttttcata aattttagta gctaattaaa aaatagttag agagtttagt
     3241 gaagtttaaa aatttatttt ccattttcca aatattttat tatcccaaat tgttcattag
     3301 aattttaaaa tataaaaact caaagtacgc taagcacttg cgcaaagatc actaagattt
     3361 ttttttacaa ttcaattttt tctataacca tttgtgttct tcgcggctcg tagtgaaaaa
     3421 agtttctact gaataatcct ggtttataca aaattagtaa caacacattt ttatacttta
     3481 ttaaattttg agggtgatta aagtaagtga agatacttaa acacattctt tcattctgag
     3541 tgaagcgaag aaccccaaaa cttcaattct aaattaatta ttagattctt cacttcactc
     3601 cgccttacag aatgacaaaa tgcgtaactt cagttaagtt aagagtcaaa aataattatt
     3661 aatctattat atttcaacca tcacatttct caacttaccc atacttttaa ttctctgttt
     3721 ttttataaaa aaaatgaata ttatcattgt aattccttca gttctaaaaa ctaaaatgag
     3781 cgttgtaaga aaaaaaaata taaaatttct gactctgtat aattcgttta ccgcaaatga
     3841 aaagattgat tttgataaat ttatcgggaa tggtttgaat gggaataaaa gaaattacaa
     3901 taagatctta tcctcactga aaactgatga aaaaggaatt atagattttt ctcagactat
     3961 taacagcaga accagatgga acagactttc cgaactaagt attcttgccg ataaatttct
     4021 tacattgaag tctttagaat acaataattt tgaaaacaga tatttattgc tgaaagaata
     4081 tgacaaaagg aatcttactt cagcctttga acaaaagtat aaacaattta aaaatgagtt
     4141 atcaaaagaa cctgttgtaa attatgatta taatatggta aatcagcttg ataaaattta
     4201 tctgaatcat ttaaaatcgg attctgattc aaagaaatat cctttaaaaa tctctgaatc
     4261 aggttataac agacttggaa tttttttaat cgaaatactg gaaaatctta tagaagtttg
     4321 gacaatcaaa aattccagcg tcttaatccc aaaaacttca aatgaggata tttattcgat
     4381 tctgagtatg gaaaaaatac ttttcctttt ttcaggattt tcaaaatcac cgaataaatt
     4441 ataccctgta attaaatttc tatatcatat ttacaaaagt ttgaatgatc ttacaaatct
     4501 agaattctat aaggaggcaa agaaaatctt ttttagagat ctcaaggaaa tttcaaaaga
     4561 gaaaagagaa caatattttc tttacatgct gaattataat atcgagctta aaaacctggc
     4621 aatttccggc gcagctgagg agttgttttt tttaatagat aaaaagctta aagaaggtct
     4681 ttcagcagat tttaaaatta aaaatttccc gttaaatcat tttcgtgaca ttatcattat
     4741 atctttaagt ctcaagaaat acaaatggac tgaaaacttt ataaaaaatt acggacctct
     4801 tcttccgccg gatatccggt ttgatactgt ttcaattggg gaagcaatgt tactatttga
     4861 aaagaatgat tttttaatgt gtaaagatct attgatgaaa attaaaagaa aaactccgtt
     4921 tagttttgta gatgtttttg tcttaaagtt aaaggtctta tatgaactga aaaattttga
     4981 tcactgtcac agcgaactta aaaaatttaa tacttactta agaaaagaac gaataaccaa
     5041 aaatcttctt atcaattact caatagagtt ttgcaaagcg tactccttat tattaaaatt
     5101 aaatgatggg cttgttaaga ataatcttat caatctgcaa tacatacttt caaaaaagat
     5161 attgatcgca aaaaaatgga tcacacttaa gatggaagaa ttaaaatatt aaaaattaaa
     5221 aaggtatttt tagtaaatta cggaacaatg gaaacgcttc cggaaatccg taagttctgt
     5281 aataattttc tttggataga cggtatatta aatattaaat attaaatatt aaatattaaa
     5341 tattaaatat taaatattaa ctattaacta ttaaatatta actattaact attaactatt
     5401 aactattaaa ctgtcaaagc aataatgatc acaataatat aaagcagagg aaatatccag
     5461 cgaaatctct tatcatattt tttagcaaga tcacctttac ctttttgtgt taaattggaa
     5521 gaagatgcag caaggacaag cgtcataaag atcaggattg atgagccgag aataaatata
     5581 tccatacgcg tcaaatatga tatccttggc agataagaag ttacaatgaa ccggtaagcg
     5641 ataagtgtca gcatggaagt agtcgcaacg gagatctgac ttgcagaaag attgggcggc
     5701 agccagaaga ctatccatga catcattatt atgagaatta atgggatcgc aaatattaaa
     5761 agataaaaac cactttccct ttgtgcagtt atattaaata ctaatgaagg cagcttctgt
     5821 gaatcttcaa ttatagaata ttcagattta gataattccc agtttacgat cttccagtca
     5881 gacaatgaaa tgtttttacc cacaccggat ctgatctccg ggtcaggctc gagagatatc
     5941 tcattcgaat taaaacttat ttcgatcaac cttaacacaa aattctgcgt gtcaaaagga
     6001 aactctctga aattaagatt ttgtgtgaag tcaccaaata ttctctgagt gtaaaaaact
     6061 gttccatctt catgtatagt tacctgttct ggaaaagatt tggatatatt aagccggtta
     6121 ctaaatgtaa gaaaaggtcc ccagatcttt tgcggatctt caagtctccc gcctttctta
     6181 ttatgcttta gtctgggatc aaaccatctt gcagtcatta caatatctgc tttaataagc
     6241 tgacttgaag catttacttc acttatatta tttaatatca ggcttatgta tactatattt
     6301 ttaccatccg ggtaaggaga agttatcaga tcctgtgatt ttaatgtata tgatgctttt
     6361 atttcattac agcatagtac aataaagaaa aataaaaata actttttaga aattcttgaa
     6421 tttgtaatca tatatttttc ttaaaaaaag aattttatgt attaaaatta attcgtatca
     6481 gagtcaccta tttgaaggtc ttttcgaata ttatacttta tctgattcaa taaatctatt
     6541 tacaaaaaac gtttatcgat aataattgat aaaataattc aaaggaataa aaaaaactat
     6601 aaacaaattc acttcgctga tgagataaaa ttctgaatgt acgtccaaaa aatattcagt
     6661 gtcaagaata tcactgctta caataaattt ttaaactgaa aaatgtataa tatggaagga
     6721 attatttttt gaaaaataag aatgagttac tttatccgag accaataaag taactcatca
     6781 aaacttatta ctataaaatt aaatttttta aggcgtaatc tttgtaataa aattaaatcc
     6841 attattatca gcatatactc cgtccgacaa atcagtaaca ttgtcaccgt ttgtatcaga
     6901 aggtatatag ccggtggcaa aattaaaact gtcgttgtct ataagactgc catcagcaat
     6961 atctataact ccgtcctgat tcacatcacc actgtataat ccaaatctca aaggagatgt
     7021 atctattttt atttgattat ttccatatgc agaagattgt aaacttacaa atttatgagt
     7081 cgcaagatgt gaaaatggac tgaatgtaac atcagatgca ctccatactc caatcgaatt
     7141 tctgtggaca gcttcaatat aataaggtgc agcttcaaca acattattga aacttacata
     7201 tgacagatta ttcaaaggaa gatagaattt tttggattca ataatattat aaggtgaatt
     7261 aaattttcta agattatatg taattgtatc tctaattgta ttatttgcag cttcattata
     7321 aaatccttct atatgtaaac ttgtttcaag aatattttct gaagcagttt tattattgaa
     7381 cctgatcccc cagcaataaa gcctgcccgt gtcagtacct gtgtcatcaa atatcgtgag
     7441 cttccatgtg ccttttgtat tcccggtaaa tgcggaattt aaatttacat ttccctgaat
     7501 ggaaggagaa aaagatgtgt aagtatttga attaggcaga gctccctgat catcaaatac
     7561 agttatgaga ttttcatcaa ctcctgcgtt ataactttca ttataaagca aagcatactg
     7621 attatttggt gcagttaata atattctgat attattcata ttttcatggt tcattgctac
     7681 aaaaacatta acgtcagtga tcggtatatt ttcataaatt tcaatttcat cttcagatat
     7741 tccggcgtct ccagaaaacg gaatcctgag atcagtctta cttaaattaa atgacccctg
     7801 aaaataattt gcacctgatc tgagacatgg tgaaacaggt ttcaaagtat tggcagctgg
     7861 atgagaaaat gcagcgttgt ttctgaagtt aagtttagtt ccctgtccgg tattggaaag
     7921 attaagtccg tcgagattat atacaacatc atcataaata ctttcattgg atttatccac
     7981 tgatctgaaa atgttttctg taatttcaga atttgttttt tcatcttcgg taattcttac
     8041 ctcgtctatg aagccattaa aatccggaag tgaaaccgaa ccgccaatat aaagtgaatc
     8101 tgaagagctt gagatcagac caatgatgtt agtactatct ataatcattt ttccattcag
     8161 ataaaaacag tacttacctg taattcctga atatgtaaat gcaacatgac tccatttatt
     8221 aacaccaata gtatcatttg aaataaaatt aaaattctgt tttatgcctg cataaagttt
     8281 tctattctca attcctaatc tgaaatttgt cactgctccg ttatcagatc ctttatgtat
     8341 aattacagag ttggaaacag atttgggata aatccaagct tctaaagtaa ctgattgaac
     8401 aggtgacaat gccgtatttg atgatgctga taaatagtca tcgtttccgt caaactctgc
     8461 actttcattg tatgtaacaa tttgtgaaag ttcattactc atatcaaacg aagtcagatt
     8521 tcttgcaaag ccattattgc ccgctcctga ataatcattt acagaaaata ccggagatgt
     8581 agattcttct ttttgaaatg gaagtgacaa taccagtccg ttatatattc ccgtatttac
     8641 tccaagagta gtataacgat acataaaaat ttctgcagca gttagtcctc tgttccagat
     8701 cctgatatta tctatctttc cggagaatat tccgcctatt cctctgccaa taccggttat
     8761 cactgaatca ttatttactg ccggagtatt tccgggttta gcgactgaag tatccagtac
     8821 gccatttata taaactgaaa atgtaccggt agctactata aatcttcctg ctatatgagt
     8881 ccatttatta ttttgaatca cagtttttcc gatcagaccg atattattat ttatcgtaat
     8941 agtcaccttt ccctgttgca gatataaagc atatcctgtt gagcttgtag aaggagttct
     9001 tttctgaata agtatctgac tgaacggatg tactgaacta tcaggattta cccagcattc
     9061 taaagtgaaa tttccggtta ggtccagtga cggactgtct ggaactgcaa cgtgagatga
     9121 atctgaagca ataaattcag atgctttatt ccagaacatc tgtgcatcag catttcctga
     9181 taaaaacaaa ttgctgatta atgtaaaaaa caccatcgaa gttgaaaaca gatttttcat
     9241 aatattaaag agtttatttt gaataatttt atttctataa ttgtttttgg atttttcagg
     9301 ttttaagtcc tctttataaa tgagattaag aggactttta cctgacctgt gagaaaggat
     9361 aatcgtttcg aatggattaa ttttattttc ttctcacagg aaataattct aatacaaact
     9421 taaatgtttt tcacctaagt tcaaaaagac aattttcatt gaatgtagga tgaaacagat
     9481 gcaaatttct taataataaa tgtaaaattt tgaaagacag caaactgata aatttgatcg
     9541 aaacctttac taagactgaa tttcgtgagt ttggtatgtt tgtaaattct ccttatcata
     9601 acagagaaaa agttatgata aatttttaca attatctaaa aaggatctat ccggttttta
     9661 gtgaaaaaaa tttagaaagg agaattgttt tttcaaaaat attttccgaa aagaaattca
     9721 gtgaaacatt gtttagaaat acagtttctg atttacttaa gctgacagag aattttgtaa
     9781 agttaaaaca ttttgaaaat gaaaaatttt acggacagta ccttctttta aaagagctga
     9841 caaacagaaa acaaatctct ctttttaaat caaattttaa aagagcagat aagatattaa
     9901 atgctgataa tgccagggat gaaatatatt ttcaaaataa atttttactg gaggatgaat
     9961 ggcgaaggaa ccatgttgta agttcgagta aaatgctttt caaagaagac aatcttaaaa
    10021 atcagtctga cagtcttcac gtttattatt taacagagtt tacaaaactt tatgcaattt
    10081 tactgaatca gtctaagttc acttatgatt ataattttaa ttacgaattg tttgatgctg
    10141 ttattgaata cctcagaaac aattttacac tttataaaga aattccatat ataaatatct
    10201 tttacaattg cgtgatgctg tataaaacag atgagagaat ttattttgat gaattaaaaa
    10261 aattacttct caggcattac aggattctaa ctttaacaga cagaaaaaat atttttgttg
    10321 ttcttataaa ttacagcagc gatcagatct cgaagggaaa ttattctttt attaaagaga
    10381 aatttaatat aaatgaacat ttgctcaaat caggtgctta ctttgaaggg aatgatttca
    10441 tgccgcatta tttttatgaa tcaattgctg ttaatgcatt agaaatgaaa agatttgatt
    10501 gggcagaaaa atttatttta aaatacaaaa aaattgtaca tccggattac aaggaatctt
    10561 catttaattt ttgtatggct gaattgtttt accgtaaagg tgatactgac aattcactta
    10621 gctttctttc aaaagtgctt cctgaaaatg taaaagacag gacaaaaatg aatattttac
    10681 ttttgaaaat tcattactat tgtaataaca ctgtacagtt caattcatta attgagagtt
    10741 caaaaaaata cattttaaga aataaaaatt tacaggaaga caataaaaaa caacttgcaa
    10801 attttctgtc attattggtt aaactttaca agatcaaaaa cgattcagat catttacacg
    10861 aacttgaaaa ccttaagaag aaaatatatg actgtacgga atttacagaa aaaaaatggc
    10921 ttgaagaaca ggttgagctc atcagattag aaaattaatt ttaacaattt atattattaa
    10981 aattctgttt taattaaaat tatataatga acaatatatt acggataata tttttaatta
    11041 tagtattaac tccattatca gagaataaag aaatttacag caggaattcg aatgatataa
    11101 atgcatttat cagcgggcgt gtagtggatg aaagaaggtt tcctgttcct aaagtaaaag
    11161 taaatattgg aaatgcagaa gtcactacag ataactccgg aaaattttct attccaaata
    11221 taagttttcc atataatgta acaattgctg aaaaaacttc tgctactgct gtgatataca
    11281 aaaatttagc cattaacaat ccggaacttg tgctattcgg tgatcttgaa aataaatttt
    11341 caaactatgt cgcgctaaac atttccttcc ctaaaattcc cgatggaggc agcgcagtag
    11401 tcaagcttat aagtacagaa acatttgagt gtaaagaagc tactgcagtt gcaggcgatt
    11461 caacaattaa aattcatgta gtatggccgg tttctgaaaa ttatattaat ggacaggtag
    11521 tttatttatt aaaggatgag aaaggatttc agactttaaa atttacaaag gttagtattc
    11581 acagaaatga cgttgataaa aaagtgacag tgagtaaaaa aaagggaaac gcgcttatta
    11641 cttcatttgt aaatgtttat cagcctactg aaaaaaatct taaaactgaa tttgatgtgt
    11701 cgctgttttt tttcaattac aatttaaact caggaattaa gtttagtgaa aattcatcag
    11761 gtaacgaaaa aacgcaagtc tgggtaccgg acaaagtatc aattacaaat aaaatattat
    11821 taagaggaag cggtttgtat aacaacggat ccggttacac gggattaaat tatgtatcac
    11881 ccggagctga tctaaagctc tatcctgaat cccctcctga attatttact ccatccgata
    11941 attatcttgg tgctaacggt aaaacaaatt tctcttattc acttggctca ggaaccggca
    12001 tttatgttat agaattcaga agcgaaaatc cacttttaaa tatttatgta gtaaattctg
    12061 acagaaaaac taatcttagt tatctttcaa gaaatgaatt ttacaatggg agttatcagt
    12121 ttacctggcg tgtgagaaag tatattactt tttttaatga aagtgaattt gtaaaaccaa
    12181 atgtttttaa aaatgacttc agttataaag cagtattgta ttcggaaaaa aggaaattta
    12241 aaaccggtta ttattgatct gtttcacctg attgtttgat agtaaaataa ctaatcactt
    12301 aatggcccaa agactcagag tattgataag aatatttctt tacttaaaat tttttaattt
    12361 taggatctct gagccatcag gcgattgaac ataagtacag gttttcaatg tttattttgc
    12421 aagtaccatt ttcctggtct gagaaaatcc gattgaagtc agtctataaa aatatacacc
    12481 gctgctcaga ccattcccgt tgaattcata agaataactt cccggagata ttttttttgt
    12541 aaacagtgtc tgtacttctt taccaagtat gtcaaagatc ttcagagtga acaataaatt
    12601 tttattttct gaaggtattg aaaattttat tttagttgac gggttaaacg gattaggata
    12661 attctgttca agtaaaaatc cttcaggaag tatctgagat tctaagacaa ttccggagac
    12721 aattccctgt ccttcagcag cattataaaa ttcatcgagt ccgactttag tatatacttc
    12781 tttcagtcct cttggccaga ttatgatcaa tgaatctatt attgctgcat ttccaaaacc
    12841 catatggacg tttagcatat tcattgaatt aaaagaattc tgcgccgaga cttctctctg
    12901 ctgccagact tcaattccgt taatggttgc ttttgcttta actctcgttc ctatcgctgc
    12961 agcattggat aaattattag acggacctga tcctatacat ttaatgttta cccatttatt
    13021 tccgttactc agttcatttt taaaaaggcc ttttgaattt gtactgccgg tcacgaagag
    13081 atcaagtgtt ccgttattat cataatcacc tgatgttgct ccgtaattac ctcctgaaag
    13141 agttaccacc tgaagagaat ccattcttgt aaaagtaccg ttcccgttat tattataata
    13201 ccggctctgc tgacctacgt cacgcgttaa aaaacaatcg aggtcaccgt cgttatcaaa
    13261 gtctccccag acatttgcaa gataataacc aatatcagat acaatatttc caacctgtgc
    13321 agccagcatt ttttcataaa caccattatt atttctgtag agattattcg gaacattgtt
    13381 tgcatagtta gttatgaatc catcgaggtc accgtcgtta tcgtaatcta tccagttcca
    13441 gacctgacca tcgagttggt cagttgccat cggcgaagtc attaatctta caagatcaga
    13501 tgtacctgtt tcagtcagca tatttttata taaaaaatct gtagaagtcg atccgcccgg
    13561 accacttccg atgaagagat ccatatcacc gtcctgatca taatctgacc agataggaat
    13621 tgtaaaaggt gcgagagtat ctgtaagtcc ggctacttca agttttgtaa aactgccgtc
    13681 tccgttattt ttaaaaaatc tgtttggatg aaatacattt ccgaaaccgt ttgcagcagc
    13741 tataaataga tcagtaaatc cgtcattatt ataatcagcc catgcgcagc cccagccggt
    13801 gttgtttagt gagtcactga tgtctcctgc ctgaacttta ttgatcacac cgttcccctc
    13861 atttttaaat aaataggaga aaggtccgcc tgttgaagtt acaaagcagt cgagcaatcc
    13921 gtcattgtta taatctgacc atgagtttcc tatatttgta ttttgatttg ccatagcagt
    13981 ttcgattttt acaaaattac cgttacccag atttctaaag atcacttttc tatttatgaa
    14041 aagatccagt aatctgtcat tgtctatatc cacccagctt gtgccgaagt aatttccgct
    14101 gagagcttca gtcactacgg gattgtttgg atcagttact tttacaaagt tctgagcaaa
    14161 tgaacctttt gcaaatagaa ataaaacaat aagtattaaa agtacagttg agggttttaa
    14221 gaaagttttt agaaaattca ttacatctcc ttttgtttag ttttttaatt ataacaaaac
    14281 taagcattgc cggtaagaat gaacatctca catgtatatg gtttttaatg ttcaattttt
    14341 aatgttcgat gtataatgct taggaagaat tgtatttaat aatgttaaat aattttttac
    14401 actatttaca taaaaggctt aagttttaaa aactacatct attcttcttt atttcaaaat
    14461 ttctccgaat aagactaatc atatcattga ttatttaaag taataatagt aatttcaatg
    14521 cttattaaaa atcaatatga aatcaggaat aagaaagaca aacaggatca ctaaattcgc
    14581 tactaaatca gaaaacgatc acaaatggta cgtagtggat gcagaaggaa agatcctcgg
    14641 aagacttgca agtgaagtcg caaaaagaat cagaggcaaa tacaatccgc agtacactcc
    14701 gaatgccgat ataggcgact gggtagtagt attgaatgca gataaaatca agatcgcagc
    14761 caagagagca gagcaaaaag tttattttac acactcacta tatccgggcg gtcagaagaa
    14821 aagaactttc aatgaaatga tgcagaaaca tcctgacaga gtcataagac ttgcagtgaa
    14881 aagaatgctt ccaaaaacta aacttggaaa caaactcata aacaaactta aagtatatgc
    14941 cggtaccgag catccgcact cagctcagaa tcctgaagta cttgaaatta gaagttagtt
    15001 ttacagattt taaaaatatt ttatttaata catacacaaa taaatgagtt caaactcaac
    15061 attaaagatc ggcagaagaa aaacatcaac tgcccgcgta attttaacac ccggcagcgg
    15121 agttatcagc atcaataaac ttgaagctaa taaatatttc aaaactgaaa atctctacac
    15181 agagatctta cagccatttg aggtgacagg aactaccgga acattcgacg cacaggttaa
    15241 cgttaacggt ggcggattta ccggacaggc aggtgcaatc aaacttggta tcgcaagagc
    15301 tttggtagaa actaacggtg aattaaaaag tccattgaga aaagcaggac ttatgacacg
    15361 tgacccgaga gctgtagaaa gaaaaaaatg cggacagcca aaagcgagaa agaaattcca
    15421 gttctctaaa agataataga aatacttctt tcggagaaat atttttcaat gctgtaaaaa
    15481 agtttacatt aacattattt aaaaatacat atttcctgat cttgttttac ctgtgtccgc
    15541 aaaagcggat gtaaaatgag agatgacggg aatagaagaa aaaacaaaag tctattcttt
    15601 tttgaaggaa tttaagaact gaaaaaatta atgaatattt cattataaga tcttctcaca
    15661 ggagacaatt attttcagta cacttaattt caaccaactc tgacctgact tttttaaacc
    15721 ccaggaggat taatcttgac acaagtacaa atcgaagatc tgttactcgc aggatcgcat
    15781 ttcggacatc ttacaagaag atgggatccg aaaatgaaga aatacatttt tatggaaaga
    15841 aacggtatcc atataattga tcttaagaag actttagaac ttcttatcga agcatgtaac
    15901 tcgatctcaa aaatttcaac cgacggaaag aaagttcttt tcattggtac aaaaaagcaa
    15961 gccaaacaga tcatcaaaga cgaagctgaa agaagcggat cgttttatgt ctgcgaaaga
    16021 tggctcggcg gtatgttgac caatttcaat actgtaagaa aaagtattaa gaagcttaca
    16081 aatattcaaa agatggagac cgacggtacc atcgatcagt ttgtaaaaaa ggaaagactt
    16141 atattatcga gagataaaga gaagcttgaa aaagttctta acggaattgt aagtatgacc
    16201 aaacttccgg gagcgatctt tgtagtcgat attaaaaaag agcatattgc aataagtgaa
    16261 gcaagaaagc tgaacattcc tgtatacgca atagtcgata caaactgtga tcctgatctt
    16321 gtggattatc cgatcccggc taatgatgat gctgtaaaat caattcagat catcacaaaa
    16381 gcaattgcag atgcagcaat agtcggtact caggttgcaa agataaaagc tgaagacgaa
    16441 tctgaagaga tgaaagaaaa attagaaaag agataatatt tattaaaaac aaattcaatt
    16501 actacaatgg agattacatt agatctaata aaagaattaa gaaataagac aggtgccgga
    16561 atcaatgact gtaaaaaagc acttgaagaa gccgaaggtg agatttcaaa agctatagaa
    16621 ttgctgagaa aaaaaggtgc agctatggct gtcaaaagag ctgataagat cactaacgaa
    16681 ggtgcagtaa aattaaagat atccgatgac cataagacag gtgctataat tgaagtcaat
    16741 tgtgaaactg actttgtatc aaaaggcgat gagtttcaaa atttcgcaga atctattgca
    16801 gcagcgactt tgagtgtaaa ctcagaagat cttgaagtaa tactctcttc aaaaacaagt
    16861 gaaggtctta ctttaaaaga taagctaagt gaaataatgg gtaaggtcgg agaaagaaca
    16921 gaatttaaaa gagcaaaaat aattaatgtt cctgaaggat tcgttaccgg ctatatacat
    16981 tttggcagta agctcggttc attgatcgga cttaaaggta catataatga agatgcagat
    17041 aaacttggaa aaaatattgc tatgcagata gttgcaatga accctatggc tgtaaaccgt
    17101 gaggacattg ataaggatct tatagaaaag gaaagagaaa tttatctttc gcaggcaaag
    17161 aatgaaaaca aacctgataa tatagcacag acaattgtca ataataaaat cgaaagatat
    17221 tttcaggaaa actgtcttct ggaacaggaa tatataaatg aatctggtaa atcaataaaa
    17281 aacctgatcg cagaatttaa taaagcaaac gataacagtt tagagatagt cagcatagta
    17341 agatttcagc ttggcgaaga aaccaaataa tttttgaaaa aacatatgac tccaaaatac
    17401 acaagaattt tacttaagct tagcggtgaa tccctgatgg gaaggcatag ttatggaata
    17461 gattctgatg tacttgaggc aatagctgaa gaagttttaa gcatttcaaa gttgaaaatt
    17521 gagatcggta ttgtaattgg aggaggaaat attttcagag gcttatctgc tcatgagcag
    17581 ggagtggaca gagagacagg tgatcagatg ggaatgcttg cgactgtaat taatgcgctt
    17641 gctattcaga atgcacttga aaaaaaaggt atcttcacga gagttcagtc agcaattaaa
    17701 atggatgaga tcgccgaacc tttcattctc agaagagctg tcagacacat tgaaaagaag
    17761 cgtgtcgtga ttttttctgc aggaaccgga agtccgtatt ttactacaga tactgcagca
    17821 gcgctcagag ctattgagat caaggcagat gttattataa aaggtacaag agtaaacggc
    17881 gtttatgatt cggatcctga aaagaatgac aaagctgtaa tgtataaaaa attatcatat
    17941 ctggaagttc ttgaaaaaga tctgagaatt atggatctga ctgcaattac tttatgtcag
    18001 gaaaattcat taccgataaa agttttcaat atgaacatta aaggaaatct taagaaactt
    18061 gtaatgggta aagacattgg aacattagtt tgctaaacag aaatttttaa taatatttta
    18121 attcaattaa tattatgata aaagatattt taaaaaacac atctgatctg atggacaaag
    18181 cagtagagca tgttcgtttg gaaatggtaa agatcagaac cggaaaagct acaacaaatt
    18241 tattagacgg tatcaaagtg gattattatg gtactccgac agctctgaat caattaggaa
    18301 atgtcagtac gcctgattat catactatta ctgtacaggc atgggataag actgcaattt
    18361 cacttattga aaaagctatt attgcagcca atcttggttt gaatcctgcc aatgacggaa
    18421 acctgatcag aatccctatt ccggctttga atgaagaaag aagaaaagag attgtaaagc
    18481 ttgttaagaa attcggtgaa gaaggtaaga tctctatcag aaatatcaga agagatggca
    18541 tagaagaatt aaagaaggct gaaaaggaag atcatttttc cgaagatgaa agaaagcatg
    18601 gtgaaaatga aatacagaaa ttaacagaca gctttacaaa agacatcgac aatttacttg
    18661 ttgtgaaaga gaaagagatc atggaagtct aaatttcccg taccatgaaa aaattctggt
    18721 ttatagttta taacttattt ggtattccgt taatttggtt gttcttcaaa ttatattcgg
    18781 ttttcaattc aaaagttaga caaggtttaa aagaaagaag aggtttgttc aatgatttga
    18841 gcagatctct ttctcattta aaaaacaata aaacggttat catacattca tcttcattag
    18901 gtgaatatca acaggcaatc ccgctcgcag atgaattcac caaaaagaat tacaatgttg
    18961 taatctcatt tttttcacct tcaggtttta ataatgcaaa gataatttca gataatgtga
    19021 taaagactta tatacctctt gattccattt ctaatcagaa aaaatttctt gatcttataa
    19081 atcccaatat tcttatattt atgaggtatg atctttggta taatattttg aatgaatctg
    19141 gtaaaagagg aattaagagg gtgcttgcaa atgcccgcta tgatgaaaat gattcgactt
    19201 ggagtattcc cgtcatcagt tcatttaaga aaaatcttta tggaatgata gatactgcat
    19261 ttgtcattga tgattttgat gaaaaaaatt acagtgaaaa gctaaaagat gaaaatacca
    19321 agatagtaaa aattggtgat tcaaaatttg aaagagtact tcagtctgta aaaattaatt
    19381 caaacactga agatgtcatt ccgccgtcag tgattaacgg caggaaagta tttgtaatgg
    19441 gaagtgcatg gaaagatgat gaagacataa tactgcctgc attagataaa tcattaagtt
    19501 ttgaaaaaga tctgttgact gtactcgtcc cgcatgagcc aaaagaaacc aagatctctg
    19561 caatagaaaa aataatttct caaaaatatg aaaatttaag atacatccgg cttagtgata
    19621 ttgggaaatt caacggtgaa aatctgatca ttgtggacag gataggaatt ttgtcaaagc
    19681 tatacggcat tgcatatctg agctatgtcg gaggaggatt taaaacggga cttcataata
    19741 tactcgaacc tgctatattt aatatgccga tcttattttc caataaaata aaaaattcag
    19801 atgaagatga gatcctgata gaaaaaggct gcggtattgt gattaaagat acaaaacaat
    19861 tttatcgggt gttcagagag tttttgacaa ataaagaatt aagagatgag accggtgaaa
    19921 aatgcagatt agtttttgaa aactccagcg gaattgctga aaagattgta aatcaaatta
    19981 cataaaagaa tatgtttaac agaaaagaat ttatcacaac ttcgctcagc gaaagtgctg
    20041 aggtaaaaaa tatgatcgca gagaaatgtt acgaaacaat ctgcgatgct tctgatctcc
    20101 ttgttaagtc agtgaaggat ggaaagaaga tactgctctg cggaaacggt ggcagcgcag
    20161 cagactgtca gcatctcgct acagaattta tgatcagact ttctcatgac ctggacagac
    20221 ccgctattgc cgctattgca ctgactactg acacttcaaa tctaactgcc ggcggaaatg
    20281 atattggatt tgaaaatgta ttcgcccgaa atatagaagg acttgggaat gacggtgacg
    20341 tactgatctg tatttccaca agcggaaact caccaaatat tctgaaagca gtttcaaaag
    20401 caaaggaaaa aaatatgaag gtcatagggc ttctcggaaa aaataacggg ggcaaactta
    20461 aagaagtctg cgatatttca attgtcatac cttctgaaaa tactcagagg attcaggaag
    20521 ggcacattac cgttggacat attttatgtg aagtcaccga gagagaatta tacgaaaagt
    20581 agcttctgtt taaaaagttg gatttgcctc gaaggtacaa agcttaaatg gctgataata
    20641 aatcccgata agacttaatc tttccgtaga gtccgaagag cacgtgactc agcggagacg
    20701 ataataaatt tgaagtttga ttaaattttt tgcttttgga aagtttggat atgaacatta
    20761 aaaaatccgg gatcagataa actgagttcg gaatttttat ttattgatct aaaaaaaaat
    20821 gttcctgctg agtccgaaaa acacgttact ctgcggataa tatatgtttt tattaatata
    20881 gttcttaaaa aattttaatt agccgaactt acctttggga attcagatct tttccctgaa
    20941 ttatcttcag cactaaaata aactttaaag tcgtccatgt ttttgtcaag attcagttgc
    21001 gctgaatatt tacctgagtt agtttcagca tccatcactt cagaaagatc catggtcata
    21061 gagctgtagg atttaccgtt gtcaaagcta taatgcattg taactgaatt cggatcaata
    21121 atatcttttg aagcaagata tgtagatata attattttat cactttcagg tttttcaatt
    21181 tgaaattcat tgctccatgc cattccgaaa taaagcaaag cgtcataagc atttacattt
    21241 ccccaaccaa gaacattatc cggattatca gattgtgatg ctgtatttct gagagcatct
    21301 ctcacctgga taggtgtgag ttccggatgt gctgaaagta tcaaagcaca aatacctgca
    21361 gtaataggag tagagaaaga cgtaccgctt acaaattcat atgtactatt atttcctgaa
    21421 atttctttga cctttgctga ataaacattt acaccgggtg ctacgacgtc cggtttgatc
    21481 ctgccgtcgc tggtaggacc attggatgta aagcttgcag gatcacctga atatgtgacc
    21541 gctccaatgg aaataattga gtcggcatcg gcagcagatc caagtgacgg ttcctttgtc
    21601 tgaaaataat ttcccattga ctggcaaaca acaactccaa ggtaggctgc ccgatctgcc
    21661 gcatttgtta tgatcgcagt attcccgtca agttcctcat atgtataaga atttttattg
    21721 tagggagcat cataagtttt atagatcaat gaactagtaa taatgtcaac accttttgct
    21781 tcagcccatt ctgtcgcttc cagccagaag tcttcttcca ttggtgtttc agattcaaca
    21841 tattctgttt tcgcaagaag cagatccgag ccaaatgccg gaccaatcaa ccttccttct
    21901 ttaaaaccgc tcatagtaga tactgtcgat gtaccatgtg aaccttggtt ttttgaatct
    21961 tcatactttt gattcttctc agcaaatgta ttaggatctt tatttacaaa atcatactct
    22021 tctataatat tcagactcat aagactctca tgatttttcc agtcaaaacc gtcatcaaaa
    22081 cttgctatca taacgccttt tccgtttata tacatgtcat gaactttcgg tacgttcacc
    22141 atttccattt gctttaaaga ttttccataa tctaatttat atgatgaatc agaaatttct
    22201 gattcaatat attcattaac aacttcaaat tcctgattta caagagtctt cacatatccg
    22261 agatgatcta caaaatccag tccttttatt ttttctactt cagcctctgt catatatgca
    22321 cttataccgt tcagccatcg ggagactgcg atcacttcaa tgccgttatt ttttatttcc
    22381 tgaatataag aattttctac aggcagatct gcataatcaa tcaagttttc ctcactaagg
    22441 acttttaatc ttctctgaac agctctttca gtgagcagct ccatccctgc atcataagct
    22501 ttactgcctg gggtgataac gatatcagat ttaaaatctc ctttgtcatt aaaaattatc
    22561 cagtactttg tatcagcagt ctcctgagaa aagatattga ccgaagaaaa ggtcagtaaa
    22621 attattaaga gtattttatt cataattaaa tttgatgtat aaaggaaaag ggaataaaag
    22681 ttttattccc ttttgattaa cttaattttc tttgttaaag tttatatgaa ttcagataat
    22741 gtgcgatctt cattacaaac attccgccga gcgcaccatc cacaagtctg tgatcaaatg
    22801 aaagtgttat gaacattgtc tttctcggaa ctatgaaatc accttcttct gtctccacca
    22861 ctacaggtct ttttttaatt gctccgagtc cgagtatcgc aacctgaggc tgattgatga
    22921 tcggaattcc gatatcattt ccaaacacac caaaattcgt aatcgaaaaa gtacctccct
    22981 gaatttcatt cagtgtcagt ttttttatcc tcgcacgttt ggcaaggtcg ttcagacttc
    23041 ttgcaatacc tataagattc attccgtcag agttatgaat tacaggaact atcagtccgc
    23101 cgttatccat tgctacggcg attccgaggt ttatggaatt tcttgtaatt gctttgtacg
    23161 gaactgaaga atcatctatg atcgaattta ttaacggaaa atcttttaat gctttgatca
    23221 cagcctcaca aatgaaaggc atgtaggtaa gattaaatcc ttctttttca acaaattcat
    23281 ttttcatttt tgctctgacc ttatctacat ttgtgagatc acattctgca actgcctgca
    23341 catgcggaga tatcctgaca gaattgatca tgtgctcagc cattttttgt ctgacattat
    23401 caaactccat tacagacaga ccttcttcgt tataatcaat tttcggttga ggtacttcaa
    23461 tctttctttc aggagctgca gccggcttgg tttctttggt atctttatta tctaaattta
    23521 tttccactga tttctttggt gactcagcag ccggtgctga agtgcggtct ttcagatatg
    23581 caagaatatc ttttttagaa actctgccat ctattcctgt acccgaaatg ttttcaagtt
    23641 ctgacattcc aataccttct acctgagcaa tgttcagaac aagcggagaa taaaatttac
    23701 tgccgctgcc tgtaatgatc ttttgtgttt cattagtctg tggagatgca gcttcgggtt
    23761 tttcagactt catttccgga acaggttcag atttttcttc tttaacttca gaggtttcag
    23821 aagatttagt ttcggctttt tcggattcgg aagttgctgc gccgttagaa cccagacggg
    23881 caatgacttt gccgacttct actacatctc cttctttgaa taaaatttcc gaaacaactc
    23941 ctgcttcggg agaagggact tccgtatcga ctttgtctgt ggaaatctca agaatagttt
    24001 catctacatc tacattgtca cccggctttt tatgccattt taagatcgtt ccttccataa
    24061 tagattcgcc cattttcggc attactatat ctacactcat tttatatttt taagttttac
    24121 ttattagttt ggttaaatta atttcaggga aaatagggat aagagatgtt gctttcaatt
    24181 caatttacaa agctttatcg acatcaaaaa gtgaactgat cgactgattt ttatttgtac
    24241 gataaatgga ttttccaaaa atttccgcaa cggaaactac ttctatcttt tttgatgtat
    24301 gtgacattgg aagagtatca gctacgtaaa gttttttcag aggagattta tttatatggt
    24361 caagtgattc tcctgagaat acagcatgag tacatccgcc ataaatatct tttgctcctt
    24421 ctgctttcag cgcatttaca gcattaacga aagttccacc ggtatcaata aggtcatcta
    24481 taataataat attctttcct ccaacatctc cgattatatc cattacttca gcgacattag
    24541 gttttggtct gcgtttatca ataacaatta aatccgcatg aagtcttttt gcataagctc
    24601 tggccatctt gatccctcca acgtcgggag aaacgattgc aagatttggt attttaagtt
    24661 ttcttaattt attcataaag acggcagatc cgtaaagatg gtccacagga atatcaaaaa
    24721 aaccctgaag ctgagcagca tgaagatcca ttgtgatcac cctgtctgcg ccggctttag
    24781 taatgagatt tgcaacgagc tttgctgtta tagatactct cggctgatcc ttcctgtcct
    24841 gacgcgcata tccaaaatat ggaatgactg cattgactct tcttgctgat gctcttttag
    24901 cagcgtctat catgatcaga agttccatca gattatctgc aggaggcata gtggattgaa
    24961 caatgaaaac atcagagcct ctgatatttt cattatactt tacccagatc tctccatcag
    25021 aaaatgtccg tatcgaacac ttgccaagtg gcatcttcag gcttgcagca attttcttgc
    25081 cgagattatt tgaaaaccta ccggcaaaaa ttttcagatc agacatcatt attatttttg
    25141 aagaaattga ggtatgccaa attaattaaa tatgaagttt aaatcaattt ataagaagat
    25201 aatttttgag agatattaat tttattttag aaaccggaaa tcaaaatcca atctgtctat
    25261 ttttacgtga attgaattta aagaggctat actctacatt tgtacaaata aatttttaat
    25321 tagggaaata tgaaaaaaat tataccactt ttctcattat tttttttgct tagttttgca
    25381 aatcagctta aatcacagat tccgcaatat actctggaag caaaaaatta tgtgctttct
    25441 gacgaataca caatgaagtt tgatctaata tttacaaaaa ctgatgatac acctttacaa
    25501 ttagcggggt ggcagttctt tttcagagtt ccccagacgt tagcagtttt ttctgcggga
    25561 tttggatcag catctgcata tatgcttgat acaagtaacg gggtgccggt ttcagacctt
    25621 cctgaatctt ttagaccaag aggatcaaat gtagttgtcg ctgcaaattc tcctggaaat
    25681 tatgaattca gattagctgc aaattctctt ccaggctgcg gaaatgggtt aataattcct
    25741 aaagacattc ctattttaat aggtagatat aacatgaaag cttccacaat aataaatcca
    25801 aacgtattgt taaataatcc aataatattc agagacagtt gcgaatcgcc actatcggta
    25861 accagaacaa agatcaattg gtatgatgca aataattgtt tgaataaaga gatgacgcga
    25921 tgtgcaaatc ataatgttga tatttcagga gtgtttcctt ttatacagat aaatttaaaa
    25981 ttgtcaattg aaggtttata taatcctgtt tttaatagat taaacagaaa agattctgtt
    26041 actgcttatt taagaaatgt gaattcacct tatcaaataa tagattcctc aaagggatta
    26101 attgattcat ctactttgtc agggaattat atattttaca acagaccagc aggtacatat
    26161 tacatatcag taaaaaataa aaatggtctg gaaacgtgga gtgaacagag cggaacaaac
    26221 tttcaaaatg gtacaaattt ttacgacttt acaatatcag cttctaaagc ttatggaaat
    26281 aatctggttt tagttggtga caagtattgt atatacagcg ggaattttaa ctacgacaat
    26341 attatagaca cagatgatct tacaatgctt gataacgatt tgtataatta tgcaggagga
    26401 aattcgctga ctaatattaa cggtgacgat attgtagata ttgatgatct tgcaattgtt
    26461 gataagaatg ccggtaactt tgtattagtt aaatggccgg gtcagacatt tcaaaaaaga
    26521 aaaaaattat taaataataa aacaattgca aggagaacca aatgaacatt ttatccaaat
    26581 tatttatttt tttgtattta atattacctg tagtttcttt ttcacaagca ccattgtata
    26641 atttaacagc aaaaaatttt tcacatccgg ctccaaatgt attgcagttt gatattttca
    26701 ttgaacatac aaatgcgcct acaacttttg aatattccgg tggtcagtac ttttttaatt
    26761 tcaatccact cattgcgaac ggaggaacat tatcttatac ttatgccgga gatacttctg
    26821 aacttcctca gcatatgaga cccagaaatc ctcagatatc cggaaatcaa ctcagacttg
    26881 caagcaattt gtttccagga gctggtaatg gctttataat gactaacaac ggaaatcccg
    26941 gtacaagaat tgtcagtatg aaattaacta ctactgcacc atctcttact aatgcaccct
    27001 tgagttttga atggagaaat aatcttcctg atccttatac aaaaatatac gcatatgttg
    27061 gtaatactaa tactaacata acttcaaatg ctgttcactc gattgattca tctggaataa
    27121 ttttaacttt caatttaaaa gtagcaattg acggattatt gaaaactgcc ttccacgttc
    27181 ataatagaag agatactgta acggtatatc ttgcaaaatc agttgcacct tataactatg
    27241 ttgattcagc aacaaacata attgattcac tatctttaag tgcaaccttc acatatagaa
    27301 acgctgtcaa cggaacttac tatcttgttg taaaacatta taattctctt gagacttgga
    27361 gtaaagcagg tggtgaaacg gtgatgaatg gtaatattta taattatgat tttacaacat
    27421 cagcttcaaa agcatatgga aataatttag cacttagagg aacattatat tgtatctata
    27481 gtggtgatgt caatcaggat tatataattg atatttctga tttaagtgat gtagataatg
    27541 atgtttttac ttatgcatct ggatttagac caactgattt taatggtgat tttgttgtag
    27601 agggaagaga tatgtctatc attgataata atagaaataa attaattaga aatccgattt
    27661 atggagattg agtaatttac actttataaa ctttaaataa atatgaaaaa aaatttactt
    27721 ctattctcaa tattttgtat gcttgtttta actaatagtt ctaatgcaca attgccacaa
    27781 tatacacttg aagcaaaaaa tttcgaactt acaaatgccc atactttaaa gtttgatctg
    27841 gtttttacca atttggattt tattcaatgg caattagctg gctggcagtt ttttttaaaa
    27901 gcacctcaga ctttaggcaa ttttcttccc ggatttggaa caagttcttc ttttgtttta
    27961 gacacaataa atggaattcc tgtttcagat atacctgaat ctttcagacc tagaaatcct
    28021 aatactgtga ttgcttcaaa ctcacctggt aattatgaat tcagaattgc tgcaaactct
    28081 ctccccggat gtggaaacgg attatctata ccagtcaatg tacctacatt aattggcaga
    28141 tataaattaa aatctacatc cataatagac attagtgttt taaatgaaaa ctcttttact
    28201 tttagagaca gttgtgatac tcctctttca gttacaagaa caaagataaa tccatacata
    28261 ttaagtcatt gtctaggagt tgaaatgacc agatgcgcaa gtcattcagt agatctatct
    28321 ggattggaaa ataatataag tcttaatata attatggcaa atgaaggact ttataatcca
    28381 ataaataata cattaaacag aaaggaatct gttacagctt ttctgcgaag cgtaaataca
    28441 ccttatcaga tattagattc ttcagtagcg tttattgatt ccgttaattt aacagcaaat
    28501 ttcatatttg agaatcatct gaatggaaat tactatatcg cggtaaaaag cagaaatact
    28561 atagaaacat ggagtaagtc gggcggtgaa ttattaatag tcggcggtaa ctcctacaac
    28621 ttcacgactt cagcatctca ggcatttggt aataatatgg tactgaacgg aagccggtat
    28681 tgtatttaca gcgggaatgt gaataacgat cagataattg atactgatga tctgagtatg
    28741 attgataatg acgtttataa ttatgtcttg ggaaatagta tcacaaatct aaacggagat
    28801 aatatagttg atatagatga tatggcaatt gtaaacagga atgccggaca gttgatctta
    28861 gtagaatggc cgggacagac aacaaataaa agaaacattt cattaaaaag caaaacattt
    28921 acaggagatt tcaaatgata aattttttca aatcaataat aataattatt ctgttttcgc
    28981 cgggaattat gttttctcaa actccaacat ttaatcttaa atcattgaat tttgtattta
    29041 catcaccgaa tgttttagaa tttgagattc gattggagca tacaaattca ccgactgtat
    29101 ttgaatattc cggaggacaa tattttttta atgttaatac tgatattgca aatggcggaa
    29161 cattatctta tacatattct tctgacagtt ctgatctccc tgtcaatatg cggccgcgaa
    29221 atcctcaggt atcaggaagt cagctaagac ttgctgcaaa tattttccca agcgcgggga
    29281 atggttttat tatgacaaac aacggaagtc cgggaacgaa gattgcaaaa atgaaaattc
    29341 agacaactgc cggatctttc gcgaatgttc cgttaaactt tgcatggaga acacagtcac
    29401 cggatccgtt tacgaaaatt tacgcttatg taggaacagt aaatacgaat gtaacttcgg
    29461 gagcttctta ccttattgac acatccggaa ttcaaacttc acttgaaatc aaagttgcta
    29521 tagaaggctt atacagacca attcagaata aacatcttaa aagagattca attaaattgt
    29581 ttttgagaaa atcagtttcc ccttatcaga ttatagattc atcaatttct attttagact
    29641 caaatacatt gttaagtaat tttacttttc gttttattca aggtggatcg tattatcttg
    29701 tgatgaagta tataaattca ctcgagacct ggagcagatc cggcggagag aatatagtaa
    29761 tcggaaatac tttaaattat gatttcacag ttgcctcttc aaaagcgtat ggtaataatt
    29821 tggttttaaa aggaagcaaa tattgtttat acagcgggga tattgataac tccggatttg
    29881 tagatatttc agatatgtca agagtatata atgacggcac aaatttctta agcggagatg
    29941 ttattacaga tctgaatggt gacagatttg ttgaccttga tgatcttgct atagctgata
    30001 ataatgtgtt tctgtttgca ggtgtaataa caccttaatt tcaatatttg aaaatttaat
    30061 tgttcaataa atttcaaact atttatgaaa aagataattt ttattttaat attaatttca
    30121 cagttgataa ttataaatga gtcatcagct cagattccgc aatacactct tgaagcaaaa
    30181 aactttaatc tggtttctcc ttatctgtta aaatttgatc tggtttttac tcataccgat
    30241 gccacggtaa tgcaattagc aggatggaca ttttttctta gaggacctca atcacttggg
    30301 actttttatg ctggtttcgg atcggcttca tctttcatgc ttgacactgc aaacggtgtt
    30361 ccagtttcag acctaccgga aagtttcaga cctaggaata caaatacaat aattgcttca
    30421 aactctccag ggaattatga attcagagct gctgctaatt cattacccgg atgcggaggt
    30481 ggtttaatca tgcagcaagg tgtcccaatc ctgatcggca gatataattt aaaatcatca
    30541 actccaatta atttatcagc tcttgatact aatcctatta tttttagaga cagttgtgaa
    30601 tcaccattat cagcaacaag aacaaagatc aactggtatg acgcaaatgg ctgtttaaat
    30661 aaagaaatga cacgatgttc aaaccatttt ataaatatga atccggtttt tgtaaatgta
    30721 aaccttaatt tgaaaatttc gattgaagga ttatacaatg tttcaaacga taaacttttt
    30781 aaaaaagaaa ctgccactgt ttatttaaga aaaagcactt ctccttatca agttgtagat
    30841 tctgcatctg caataataga ttcagttaac ctaacaggta acttcgattt tcaaaacatt
    30901 tctttgttct ccaattatta tatagtcgta aaaacaaaaa acggtcttga gacctggagt
    30961 aaatccggcg gagtaacact ggcagaagga aataatttct atgacttcac ttcctcagtt
    31021 tcgcaggctt atggcagcaa catggtattg aaaggaagcc ggggctgtat ttacagcggg
    31081 aatgtgaata acgatcagat aattgattct gatgatcttg ttatgattga taatgacctg
    31141 ttcaattata cattaggaaa tgcagttaca aatctgaacg gtgatattat tgttgacata
    31201 gacgatcttg caatcgctga cagaaatgct gcacagctta ttcttgtaaa ttggccggga
    31261 ttggcatttg ataaaagaaa agagcttgaa aaaaaatcaa tatttaaaaa tcaaaagatt
    31321 tttgtaaaaa actaaatccg attacttgta aaagaaaaaa taaaacaccg aca
//
LOCUS       DAORVQ010000016        30761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_482_length_30761_cov_5.305217, whole genome shotgun sequence.
ACCESSION   DAORVQ010000016 DAORVQ010000000
VERSION     DAORVQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 30761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30761
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_482_length_30761_cov_5.305217"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PLX80_02620"
     CDS             <1..1382
                     /locus_tag="PLX80_02620"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQY19721.1"
                     /translation="NQTYMTAIQSQVANLEVQRDIELAPIQDEQVREKTRQAYDRKLD
                     NLKEKLAQQIEIMKTGIQASTPAEKRELSSDIFKQKLEIQDMRIKNAYYKDLINKSQA
                     ELKNLPEQMSELVKLERDRSSAEKLYTTLEQKYQEATINERARVGNASILDLGLDNNA
                     PVAPDRPKIILFGILIGLGLGLAFAFVRNYLDKSIKSPDELESKGASVLSWIPKIESI
                     GKNGVSTPEFIVGNKSNSAASEAFKALRTRIQFSKLESEQLKTILVTSSIPFEGKTIV
                     SSNLAGSFAQAGKKVLIMDCDLRKPRIHNVFETDKFPGLSDYLFTNVTLEDITRPTPM
                     DNLHFITSGTIPPNPSELLGSVQMKQFINKLKEIYDIVILDSPPFITVTDSEILYNIT
                     DGTILVCQANKTPADAFWKTYDRLNKKYPHHLLGCVLNNFNFKSSYGYYYNYYYYYSQ
                     PDSNKKILK"
     gene            1535..2389
                     /locus_tag="PLX80_02625"
     CDS             1535..2389
                     /locus_tag="PLX80_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY19722.1"
                     /translation="MSENSQEEWTLEIKPVSGWFNFHLKDVWRYRDLLMMFVRRDFVA
                     VYKQTILGPFWFFLQPILTTITFTIVFGNIAKISTDGIPPILFYMSGIVSWGYFSDCL
                     TRTSSTFVTNANIFGKVYFPRLVSPLSNVISLLMKFSIQLFLFICFLIYYSFNGGNVN
                     PNWYILLTPVLLLLMAGLGLGFGIIVSSLTTKYRDFSFLVSFGVQLLMYATPVIYPLS
                     ALPEKYKWIVLANPMTAIIDTFRYAYLGAGTFNAGNLIYSSIFMVVILSFGIVIFNRV
                     EKTFMDTV"
     gene            2400..3725
                     /locus_tag="PLX80_02630"
     CDS             2400..3725
                     /locus_tag="PLX80_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19723.1"
                     /translation="MSSVIKVEDLSKLYRLGMVGRRTIAEDLNRLFARLTGKENPYLK
                     VGETNTRNKKGLSDYVWALKNINFEVGQGERFGIIGKNGAGKSTLLKILSRVAGPTSG
                     KIKIKGRVASLLEVGTGFHPELTGRDNIFLNGAILGMTQEEIKSKFDSIVDFSGVERY
                     IDTPVKRYSSGMYVRLAFAVAAHLEPEILIVDEVLAVGDAEFQKKCLGKMHDVSEKEG
                     RTVLFVSHNLQAIRNLCQKTLILNDGEVFTNGETEFALREYNELTRDIKIDANTEIGN
                     NVNRRGKGRVRFTSIELLDESGNKRFKFELGEKIRVKITYEIFKEIDNLIFVFALNSG
                     LSRECLTLVQFDLSEKKMLPGLTGEVIVEIPELKIKPGEYPLYFELTDFNHNSFTKDV
                     IDDLTAPLEIIEKKSKTDTGRPTISGYFKSEASLLKNEIFESKKLKKAL"
     gene            3783..4763
                     /locus_tag="PLX80_02635"
     CDS             3783..4763
                     /locus_tag="PLX80_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19724.1"
                     /translation="MSKEIDIGFLNRTPPQYSGSLILNILGFQIFRTLFYNFWRLRPQ
                     KNKYKEYTETLERDGILVIPDFFSNEQFEEIKKEYEQLYSDWDPFEFDPLTLNNRQKN
                     FPEYFETIAEKFISPNTPSFLKYFLNNDIINELTKAVIHRKIRVKPYHLFWHLQKRNP
                     DKNVDSLHFASFPHADVPYPTIKVFLYLRDTDESNAAYVFAKGSHKLTLKRLINEYKF
                     SIRYSKDGSDNVTELELNKMGYRSESICGKANTLFISNNMGFHNRGKFTSMNPRVTAQ
                     LDFRNIESWRNTLNRGGSNIISRLSKKFVKSIDNKTKSKISAGIGNNHMS"
     gene            4732..5388
                     /locus_tag="PLX80_02640"
     CDS             4732..5388
                     /locus_tag="PLX80_02640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQY19725.1"
                     /translation="MQVSVIITCHNYARYLSRAIRSALNQSLDKKEYEIIVVDDGSTD
                     ETQNVMKTYNQKIKPIILDKNYGLAAARNEGIKSALGRFVINLDADDYMDENLLLVES
                     LFLNSNANWDAVSCDYILVDENEKHLERISGKKKHIACGVMFRTDLLFEIGLYDEKFK
                     MREDEDLRIRFEKKHNIYNIELPLYRYRKHDNNMTNNKLGMTKFKKVLDTKHKPRKNK
                     "
     gene            5398..6138
                     /locus_tag="PLX80_02645"
     CDS             5398..6138
                     /locus_tag="PLX80_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQY19726.1"
                     /translation="MKTVAFIAARMGSERLPGKVLKPLSGIPSLVHIFNILREVKNID
                     DFAVVTTILKEDDIIEKICNESGVTCIRGSVHDVLDRFRTASEITNADVIVRVTGDDP
                     LMDPQIIDKVIFEHRSGDFDYTSNIEDRTYPRGMDTEVINFGALEKCWRESADKDDHE
                     HVTLYIRRNPEMFKIKSVKKDGAPASDLRLCLDTEEDYLLAEKIYEKLYDGKPIRLDE
                     VLDLLEKEPLLRKINESVLQKKVKDKIF"
     gene            6160..6822
                     /locus_tag="PLX80_02650"
     CDS             6160..6822
                     /locus_tag="PLX80_02650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HQY19727.1"
                     /translation="MIKLPDFNKAYDYENEFYLSCDKSRIGKLLAHFELYNMTKSAEG
                     DIVECGVFKGASFVRFAMFRNLFEENSEKKLIGFDSFGKFPGTDFEADKKLRENLIKD
                     AGENSISTDQLIDVLKNKACEKNVELIAGDITVTLPEYTKKNPDLKISLLNLDTDIYE
                     PSVTILEELYPKIVSGGILVLDDYNVFPGETKAVDDYFKGVKIEIKKFPFSATPCYII
                     KP"
     gene            6843..7805
                     /locus_tag="PLX80_02655"
     CDS             6843..7805
                     /locus_tag="PLX80_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY19728.1"
                     /translation="MNFLICGLGSIGSRHARILKSIGDNNIFAFRTGNSKINEFDFQY
                     TEINSLNELDKYNIDGVLITNPTSMHIETALSIAANGIPMFIEKPLGNDMTSLVELQN
                     IVRTKNIPVLIGYNFLHHPGIRLIKKLISENKIGKVISARSQFGTYMPDWHKYEDYKK
                     SYASNSSMGGGVVLTSIHEQNYLTDFFGNITEVKAMRTGEGILGIDAEEGAEILLRHE
                     SGIVSNIHLNFYQKPYYRNCQIIGTEGTIYWDFMKPNLTLLYQDKTEIIKTGKGAYEL
                     LDESYTEQMKHFTEVIKGNAKPVADLNRGVKDLETALEILKQIN"
     gene            7814..8635
                     /locus_tag="PLX80_02660"
     CDS             7814..8635
                     /locus_tag="PLX80_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY19729.1"
                     /translation="MQLKTTDPGKINFKEKFELKDKVIIITGGMGLIGSSFSEACCQF
                     GANVVIADREGAGSEEHAKTLSEKFGREMTGVTIDVAKRADVERLKNTAIEKYGRIDG
                     LINCHQNKTAKFFLKFEDYTDENWDAVVETNLKGTFLTCQIIGGYMAENGHGSIINMP
                     STYSVVAPNHNLYKDTSLGTPAVYSASKGGVMALSQYLATYWANKGVRVNQITPHGVW
                     NNHEDQFEKNFSEMSPLQRMSYNHEVAGAAVYLLSDASSYVTGHNMVVDGGWTTW"
     gene            8744..9796
                     /locus_tag="PLX80_02665"
     CDS             8744..9796
                     /locus_tag="PLX80_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQY19730.1"
                     /translation="MKITEIFNTYDPLIINRPYVIAEAGVNHEGDFELAKRLINEAAE
                     GGAHAIKFQTYKAETLASKDSPYYWDLNKEPTESQFELFKKYDKFGKKEYELLKSECD
                     KAGIEFMSTPFDTVSADLLNDLMSVYKISSSDITNIPFIEYMCRFGKPILLSTGASDT
                     DEIKKAVDVINRHGNKLCLLHCVLNYPTLDENANLGMILDQKKIFPDAVPGYSDHTLP
                     GNMDVLKTAVLLGAAVVEKHFTFDKTLPGNDHYHAMDKEDLKNFFREMERVFLITGDF
                     KKKALGSEDAAIKNARRSLVSSKKINAGEVISESHLTWKRPAAGISPSEINKLIGKIA
                     AEEIEEDTVLKWEMFI"
     gene            9877..11304
                     /locus_tag="PLX80_02670"
     CDS             9877..11304
                     /locus_tag="PLX80_02670"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HQY19731.1"
                     /translation="MTNKDQIIEKGNGLPVKIYITFGIIAILSLIPFLLLNYYNQPTS
                     EDFYYSGELFTFGFSKAFRVLYKFWGGRYFGYLLILVNPLCFNSIAGYKFATFFMMIL
                     YFVTIHFFISRFTRNSLNLNERLLLTLSIVFVCLYSMPSIGQGFYWLISVLFYNVGII
                     LSFLFLIVYSNQQNAISKSKKLLYMIPCVIILGALIGSCEIVGGTMFMLLTALLVINF
                     IKNRSVNYYTLIYLILCLIGVLIVSSAPGNVSRALQYPGNHDFLFSFTNALSFASLEI
                     LKWTFITPLLLVTFLLLPVFKKLINDNSLNDVFKINPLIAFVLFAGLMVVNSFLIFWS
                     IAEAPYGRILNFIYLIFLICWFYNIVVFLYYFRNKFNLFTARIPNAAFGIALVLALTF
                     GLKDNNIKTAYSDMFSGKASEYDKKVNERYEHIYNSNSDTVYVKSILNVPESFFQHDI
                     YEKAETMYNEGYAKHFKKNTILLKK"
     gene            11307..12086
                     /locus_tag="PLX80_02675"
     CDS             11307..12086
                     /locus_tag="PLX80_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQY19732.1"
                     /translation="MIKDKKIVASIEARMTSSRLPGKVLMEINGKPVLEILIDRLKRS
                     KYINEIVIATSSNDADDRIEELGKELNVAVFRGSEDDVLGRVVGAVETLKGDIIVEIT
                     GDCPLMDPEVMDSVIEEYIENYPEYDYVTNIGYVENEVREIPIGMDIRVFTFKDLKYI
                     SEITDDPEDREHVSLYFFRTGKNKYKLFNIKTPDKWKRNYSVRLALDTKEDFQFIEKI
                     YTELNKINKEFGLEDILNFLDENKELLKINSEVIQKTVSNL"
     gene            12083..13069
                     /locus_tag="PLX80_02680"
     CDS             12083..13069
                     /locus_tag="PLX80_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY19733.1"
                     /translation="MSKVNKYSAAIIGCGDIGYRFDADWSDDREGALTHFRALKNSDK
                     FEFISVAEKNQETLDLISSHSNISCYENYINMLSEQMPDIISIVTNDESHYKILNDIL
                     YIKDYKPKFVFCEKPLALNLDEIESICEKYSEAGIPLQVNYTRRFLNEFIEINKDIDK
                     QNTGRIESVTFYYSRGLVHNASHYIDLALWYFGIPDEVLSFSSKKGITDSDKSVSFIL
                     KYNEGPELIFIGLDTGKLSFAEIDIVGTAGRIKFNFKNEIERYEVISNPVFKGYTSLN
                     LESSVPVKFEYALPNAYENIFDVINNKQELHSPAENSIKIFDLINKIRENRI"
     gene            13066..14385
                     /locus_tag="PLX80_02685"
     CDS             13066..14385
                     /locus_tag="PLX80_02685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY19734.1"
                     /translation="MTKLALTGGTPVRTKIFPQSNTIGEEEKKAVMKVLDSGNLSQYV
                     GTWNPDFYGGPMVKVFEKNWADAFGVDYCMAVNSNTSGLYACMGACDIGPGDEVIVSP
                     YTMTAGAIAPLIYGAVPIFADIDEDTFCLDPKSIEANITPRTKAILLVHILGHPSDMD
                     EIMAIAKKHNLKVIEDCAQSPMSTYKGKLVGTIGDVGVFSLNYHKHIHTGEGGMIVTN
                     DPVIAEKIQLIRNHGEAVVAGKKTKDIKNIFGFNYRMTEMEAAVGTEQLKKLPGLIEK
                     RIEYADYLSGIIGKLPGITPPLVKEGCKHVYYLQTFKFNKEIIGIHRNTFVDALRAEL
                     PTMVLRENSPIIGCGFAAPLYLQPLYQQRLNQCSFNCPRYEGNVSYEKGICPVTERMF
                     YDEVFTHEFMRPSFSKADLEDVVNAYQKVYENVNELKEYESKNSVKV"
     gene            14386..15447
                     /locus_tag="PLX80_02690"
     CDS             14386..15447
                     /locus_tag="PLX80_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HQY19735.1"
                     /translation="MSKKNILIIFVDPGGGLVVSELAKKLDADPEYNLNVYCGNLSKV
                     ISENAGLNFKIISDMINIETAEEIVNTVSPDMILTSTGRGNTEQLFRNIAYRDSIPSV
                     VILDFWKDYRRRWLYADHNIPELKDIVCVMDELTKKEMTDEYFPERNIRVTGHPYLDK
                     IFKSKRDLIEKEIKNDFNILFLSQPLDIIGIHNYEIHPFRKLINAVSEYSEKSDLKIN
                     LKIKLHPSEKMSEDLSKILEEKEIKKVTVIHVNNEAVHELIEQADLIIGYNSIALFEA
                     RSMGKRAVSLKLFPINDSLMKAFENAGIESSDANSESLFNLLDEKKETYISDNIFEGG
                     IKNCIRVISEELKVKEKVS"
     gene            15459..16025
                     /locus_tag="PLX80_02695"
     CDS             15459..16025
                     /locus_tag="PLX80_02695"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQY19736.1"
                     /translation="MINPFLIGNKIYLSQLSQNDISDRYINWLNDEEVCRDNSHARFP
                     NTRSKTLSYIEHLEKSKDETAFAIRWKKNDLHIGNAAIQKIDRINRSAELAILIGDKK
                     YWGKGIASEVYELLIKYGFDTLNLNRISSGQTIANKGMIKVCERSGMKKEGILRQVLY
                     KQGEYLDAAVYSILKREFEKVKKHIKNK"
     gene            16022..17221
                     /locus_tag="PLX80_02700"
     CDS             16022..17221
                     /locus_tag="PLX80_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HQY19737.1"
                     /translation="MSEIKLNEPRMTGVQYCIRCCIPQTQEGVKFDEMGICQACQSSE
                     QKIHIDWVEKEKQLREILDKAKDLSGNNYDCIVPISGGKDSTFQLHVLTKIYKMKPLA
                     VTFSHNWFSETGWYNLLNSLEKFNVDHIMFTPNRDLVNRLAKKSLEAIGDTCWHCHAG
                     VPAFPLQAAVRFNIPLLIFGESIAESSGRSSYKNPLMEFNVDYFMKVSRKRDPQDMVC
                     DYISMKDMKPFFYPSVQELEESGVRLMHLGDYIFWDDERQMEFVRDTYGWKETEIEGT
                     YKGYKSAECIMPGMHDFTNYLKRGYGRSTYQASVDVRNGLLSREEGFELANKYDMEIP
                     ESLEYYLKITGMSKEEFYKLMKEKRLDEIKNIELPVIQKTRKNKERILPLAQQLIEEE
                     ELRRANK"
     gene            17223..18596
                     /locus_tag="PLX80_02705"
     CDS             17223..18596
                     /locus_tag="PLX80_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQY19738.1"
                     /translation="METSVIEKNIFLQKSISEITESLRNNLITPLEIAEACIKRIEEY
                     NEKYKVWIIYDKKILFDQAEKITERLNTGCNIRKLEGIPVGIKDIFNTYDFPTQMGSP
                     LWQNFTSGNDSRIIFYLKQQGSIIPGKTDTAEFAVHEIGNCLNPHDITRTPGTSSSGS
                     AVAVALGMVPAAIGTQTAGSIVRPASFCGVYGCKPSFGLIPRTGMLKTTDSLDTVGFF
                     TSRYEDLETVFHVVRVHGSNYPFSHNALTDEQRQNKPSERKWRVAFAKTHTWDFAPEY
                     AKKAIEELVNKISLTDDIEIEEAKLPEITKQSHEVHQMIYDKTLSYYFQEEFKKPEFI
                     SPVMNEIFERGNKITVDQYHTSLKDQEKIAFAMEEYFKDVDIVISLSSAGAAPLRNEN
                     EKPDPSLMWTLSHLPVISCPVFVSPDGLPFGIQITARKYNDLLLFRFTDHIRKNGLIP
                     EGVNPIL"
     gene            18635..20281
                     /locus_tag="PLX80_02710"
     CDS             18635..20281
                     /locus_tag="PLX80_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19739.1"
                     /translation="MNPISIVKSRLFRLKFIKDLNRKKKINKLYSKGVNSGKNSEKVL
                     FWTTGGMIVQTNIEGMLAASLKLRGNKVHVVLCDGVYKACAKRVDHPDLPVKEWGKFC
                     KSCIRQNSAVLDNLGVEYSYVSEHITPERIYELRKTSLETDLTNYKSISLNGYALGNN
                     LESAMIRHTKSSNFIGQEEILKEYSFTVLVVAEASSNILKKFNPDKVFMSHGIYADWG
                     PALTTSLSKNITINSYICSYLNVHFQFGTVKKMNETFLTLHDNAWNIIKAEKLNENHK
                     RKLSKFLSGRYKENIAHDMRGLLKEYRGDTEHLYSKYNLDKNKPVWGIMTHINWDAVS
                     DYFPMIYKNFDEWLGETINIIKEVTEVQWLIKIHPSELNDNPDTGCQKYIEKNFPDLP
                     DHIKVITMDDDISPLDFYNLLSGGVTVLGTGGLELSLMGKPVILAGEAHYSGKGFTYD
                     GIDKNKYAELLENTSEIKPLSKEMHELAMKYAYTYFIRKQIPMPPTIKDDLLIDFTKL
                     EHLLPGKNKHVDFLCERILDSEDFILPENLVSTLTDSKPN"
     gene            20424..21413
                     /locus_tag="PLX80_02715"
     CDS             20424..21413
                     /locus_tag="PLX80_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY19740.1"
                     /translation="MKISVIVTTFNRAKIIPMCLDSLVVQNYEKSDFEIIIVNNNSSD
                     NTEEVIEDYIERYPEINLKYYFVPRPGQVYARQIGILAASNEILTFTDDDGILVKDWL
                     KEIANVFSMSDEVVGVAGKIKIKWDEDPPEWIFDYERQLGKLDYGKDIKFEVGLYMNA
                     GNLSIKRDVLFEVGGFNPEMVGEWLMGDGETGLWKKLFKKKYLIGWAPDAVMYHYQVA
                     KKNATVEDIERRFYNNGICIPYNIYAIDQEGKKALLKNMFYASKQWVRWIIKKNRFGI
                     LDNKKSEYYAKFRSAFYFAQIRYTLNILFSREFRNKLLSDDWKINLNNTGKPL"
     gene            21410..22708
                     /gene="aepX"
                     /locus_tag="PLX80_02720"
     CDS             21410..22708
                     /gene="aepX"
                     /locus_tag="PLX80_02720"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006670254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HQY19741.1"
                     /translation="MKKVYVGMSADLIHPGHLNIINEAKKLGEVTVGLLTDKAIASYK
                     RLPALTFDQREIIIKSIKGVENVIPQETLDYVPNLQKLKPDFVVHGDDWKDGVQKQTR
                     QRVIDALKEWGGELVEIKYTEGISSTQLNQTLKDVGTTPDVRMKKLKRLMGVKPLLRI
                     IEAHNGITGLIVENIFSKRDGVKVEFDGMWLSSLTDSTAKGKPDTETVDVTARLHTLN
                     DIIEVTTKPIIYDGDTGGRTEHFAFTVRTLERLGVSAVIIEDKTGLKKNSLFGTDVDQ
                     TQESIENFCEKINTGKRSRVTEDFMIIARIESLILKAGLEDALQRAEAYINAGADGIM
                     IHSKEKSPDEILEFCREYEKFESKVPLIAVPSSYSSIYESELEKAGVSIVIYANHLLR
                     SAYPAMIETAKSILENGRSYETDNNMMSINEILNLIPGGK"
     gene            22712..23839
                     /gene="aepY"
                     /locus_tag="PLX80_02725"
     CDS             22712..23839
                     /gene="aepY"
                     /locus_tag="PLX80_02725"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HQY19742.1"
                     /translation="MKPADFFKLLSENKIHFYTGVPDSLLKNFCSFVNDNVSPENHII
                     STNEGSAVALAAGHYLSTGNPGLVYMQNSGFGNAINPLLSLSDTEVYGIPVMLMIGWR
                     GEPGKKDEPQHIKQGRVMIELLKSLEIPFRIFSSENNEVKKDIKELYEISISNNCPAA
                     MLISEGFFESYKSENNVENNYPVSREEAIKLIAGLTNEDDIIISTTGKTSRELYEFRD
                     EKIFGHQNDFLTVGSMGHSSQIALGISLNKKNNNVFILDGDGAVLMHMGSLALIGSEA
                     PENLKHIILNNGAHESVGGQPTAGFKISFTEIAKACGYKTTLLAETREQIKNDFNVLK
                     NSFGPSLLEIRINKNSRSDLGRPSSSPMENRNDFMKFLNQL"
     gene            23841..24977
                     /locus_tag="PLX80_02730"
     CDS             23841..24977
                     /locus_tag="PLX80_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HQY19743.1"
                     /translation="MTQKVIIENGSIKKLTDLIDIQIVKRIFLVTGKNSYSISGAERK
                     LKILDRNFETVHFDEVGEIPEIENVKKGIELFNESDSDIVIAVGGGNVIDIAKSINIL
                     STHSETAEDLIMGKKNISIKGKDLIAIPTTAGSGSESTHFAVVYSGIDKYSMADEFIL
                     PDIAIVDPELTYSLDSRQTAISGIDAFCQAVESFWSVNSTKESREYSKESIILILKNL
                     KKCVKDPHESARFNMCMASNLAGRAINISKTTAPHAVSYTLTSEYGIPHGQAVCVTLP
                     GFLEFNYNLNDENISEGINKEEHKVLMDELSKILGFKSNSDAKKFLIEFIDSLGLKIN
                     LTDLGIKSSEDINIISKNVNTERLKNNPRKISESDLEKILSDIL"
     gene            24977..26176
                     /locus_tag="PLX80_02735"
     CDS             24977..26176
                     /locus_tag="PLX80_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HQY19744.1"
                     /translation="MNKGNGKGTSRFKIKSGMVFDKVKGKKILILDIEELSFIPYIFP
                     IYESLKKKTNLISFYISTFYPGAEELNVFEIPLNKQYHVKFNSELSEADIFLSPHIYG
                     IGNERTLRIHINHNQPVKYQSYMKNDFKNFDMHFLTSPLHREQTENTIRDYSLEEMNI
                     KLFDIGYAKSDQLLNGAFNRENVLNDLGLDPNRKTILYAPSWDEGLSLRMKGENVIEE
                     LLKINNVNVIVKLHPISYCPADGPNYEFYTGGINWKERLEKFDSNENFKNVTTGKADP
                     LLAAADVMVTDLSSVALEFILLDKPVIYIDCPEFFERTIEKTYSGFGNTTAEFVKNDP
                     KANAGRHTGIVIENVSELQMAVDRALKDPFELSNKRKELAKKLTYNPGRAADTAAEKI
                     LELLDLN"
     gene            26240..27523
                     /locus_tag="PLX80_02740"
     CDS             26240..27523
                     /locus_tag="PLX80_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HQY19745.1"
                     /translation="MKKEIEKYFDRLWPICRSLTGDGNRETLKILSEIADIKVTEVPS
                     GTKCFDWNVPPEWNVKDAWIKDSNGKKIIDFKKNNLHLLGYSEKFNGKLNLDELKNHL
                     HTLPDQPNVIPYLTSYYSSRWGFCLTHKQFKGLKKGEYEVFIDVKKDPKGSMTVGEAV
                     IPGQSKKEILLSTYICHPSMANNELSGPLVSAFLFKELKKKKDRRYTYRFLFLPETIG
                     PIWYLSKYGNDLKKKLQAGYVLTCIGDKGNFTYKKSRAGNTLADRAAELTLSQNEKKY
                     LIEDFFPTGSDERQYCSPGFDLPVGSLMRTRYGKYKEYHTSSDNKDFISFDKIEKSVN
                     MYMEILYVLEGNRYYLNTKPYCEPQLGKRGLYPTLGSQKATTEFVNAMMWVLNLSDGA
                     NDLISISIRSGLKFNVIEEAARRLHEKGLLKIYLK"
     gene            27531..28550
                     /locus_tag="PLX80_02745"
     CDS             27531..28550
                     /locus_tag="PLX80_02745"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY19746.1"
                     /translation="MPERKNNYRSKRYLITGFSGFVSRNFIEYLNSKNEDFEILGIDI
                     KIPDFEYEFYNNLKINFQKTDLREREKLCKLVYDFQPDYILHLASYSSVAGSWENPAN
                     SFQNNLNIFLNLLEVVKTIRSMCRIILVGSSEEYGDVKISDLPLVEEKIPNPSSPFGI
                     ARLSQEMLAKLYVEVHGLDIILTRSFNHIGAGQGENFAVASFTKQLVELKFHSNGDFV
                     IAGDVSIVRDFIDVRDVVRAYYLLFQEGRKGEIYNVCSGYGISLQNIINIMSDILNLN
                     ITIKEDISLRRPKDNSVIIGSNKKIKSEIGWENEISLEDSLSNMINYYLHYSEISASN
                     KLPTL"
     gene            28547..29830
                     /locus_tag="PLX80_02750"
     CDS             28547..29830
                     /locus_tag="PLX80_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19747.1"
                     /translation="MSTVIKVEDISKLYKLGIVSSKSIVQDVNRFIARITNKEDPFSK
                     LGEINDRTVKRNSDYVWALKDINFEVKDGEILGIIGKNGAGKSTLLKILSRVTSPTKG
                     NIKIKGRVASLLEVGTGFHQELTGRENIFLNGSILGMTRAEIKSKFEEIVEFSGIGSY
                     IDTPVKRYSSGMYVRLAFAVAAHLDPEILIVDEVLAVGDAEFQKKCLGKMQNVSQNEG
                     RTVLFVSHNMAIMSKLCNNVIVLKNGQVDFPKGPSDEGIQHYILERKEKAEINLSKRT
                     DRTGEGKVRLTDFAMMNDRNEKIDIAISGQKVKFRIHYECIENPDPRNITVAIAVSRS
                     DGFLVTVLGNDISSRGFDSISQEGYFTCVLNKLPITSGSYLLNLIVYQNGTRQDWIQE
                     AVTITVEDGDFYNTGKIVPSTHRSVLLEHEWSLND"
     gene            29846..30562
                     /locus_tag="PLX80_02755"
     CDS             29846..30562
                     /locus_tag="PLX80_02755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19748.1"
                     /translation="MKKIVLGFFALFGIGLYKIKKEVVKKPKSEYKPIKNFEVTSAFE
                     HNTAERMNDFFSDPEHVENYISDVRVDFYKQITEYFNENKIDLNNKEVLDVGCGTGHL
                     LMYLSDHYKMKKVYGIDFSDAALAVAKKNLPEGEFMVHDIYKDHDKKYDIVLCTEVLE
                     HLVDPEDALSNLEKMMNENSLLFITTPNGRTDTYKGHINYWGPEGWEIFLRKNCSQKN
                     VKTGLLVDSRFNYACIKNAE"
     gene            30578..>30761
                     /locus_tag="PLX80_02760"
     CDS             30578..>30761
                     /locus_tag="PLX80_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19749.1"
                     /translation="MKKHSRKILILSYSDLSKDPRVLRQINALKEKNSITACGYKPSD
                     IDGIRFFSIYPKNESLA"
BASE COUNT        11332 a         4260 c         5953 g         9216 t
ORIGIN      
        1 taaatcaaac ctatatgact gcaattcagt ctcaggttgc caatcttgaa gtccaaagag
       61 acatagaact tgcgcctata caggatgaac aggtcagaga aaaaaccaga caggcttatg
      121 acaggaaatt agataattta aaagaaaaac ttgcgcagca gatagaaata atgaagaccg
      181 gtatacaggc aagtacacct gctgaaaaaa gagaactcag ttctgatatc tttaagcaaa
      241 aattagagat ccaggatatg aggataaaaa atgcatacta taaagatctg ataaacaagt
      301 ctcaggcgga attaaaaaat cttcctgaac aaatgagtga actggtaaaa cttgaaagag
      361 acagaagttc tgcagaaaaa ttatatacaa cgcttgagca aaaatatcag gaagctacaa
      421 tcaatgaaag agccagagtc ggtaatgctt caattcttga tctgggttta gacaataatg
      481 caccagtggc accagacaga cctaaaatta ttttattcgg aattttaata gggcttgggc
      541 ttggattagc atttgcattt gtaagaaatt atctggacaa atcaattaaa tcgcctgacg
      601 aactagaaag taaaggtgcg agtgtacttt catggattcc aaaaattgaa tcaattggaa
      661 aaaatggagt atcaactccg gaatttattg ttggtaataa atcaaattct gctgcatccg
      721 aagcttttaa agctttaaga accagaattc aattctccaa attggaatct gaacagctta
      781 aaacaattct tgtcacaagt tcaattcctt ttgaaggtaa aaccattgtt tcctcaaatc
      841 ttgccggaag ttttgcacag gctggaaaaa aagtattgat aatggattgt gaccttagaa
      901 agcctaggat ccataatgtt tttgaaactg ataaattccc gggtttgagt gattatcttt
      961 ttacaaatgt tacattagaa gatattacaa gaccaactcc aatggataat cttcatttca
     1021 ttaccagcgg aactattcct ccgaatccat ctgagctgtt aggttcagta cagatgaaac
     1081 agtttataaa taagttaaaa gaaatttacg atatagtaat tctcgattca cctccgttca
     1141 ttacagttac agactcagag attttatata acataacaga tggtacaatt cttgtctgtc
     1201 aggcaaataa gacacctgca gatgcattct ggaaaactta tgatcgtttg aataaaaaat
     1261 atccgcatca tctacttggt tgtgttctta ataatttcaa cttcaagagt tcttacggat
     1321 actattacaa ttattattat tactattcac agccggattc aaataagaaa atactaaagt
     1381 aaccggaaaa taaaatttaa tttctaaaag atcacacatt ccgatataaa tgaaatttca
     1441 tattttgaaa ttttactttc tcgattcaag cagattgtaa atatactgtc tgttgtttac
     1501 atcaaatttt attaaaatta ttttaaatat ataaatgtct gaaaattctc aagaagaatg
     1561 gacattagag attaaaccgg tctcaggttg gtttaacttt cacctgaaag atgtctggag
     1621 atacagggat ctactgatga tgtttgtcag aagagatttt gtcgctgttt ataaacagac
     1681 tattttaggt ccattctggt tctttcttca gcctatactc actacaatta cttttacaat
     1741 tgtattcgga aatattgcaa agatctcaac tgacggtata ccacctatat tgttttacat
     1801 gtcaggaata gtaagctggg gttacttttc agattgtctc acacgcactt catcgacatt
     1861 tgttactaat gctaatattt ttggaaaagt atatttccca agacttgtta gtccattatc
     1921 aaatgtgatc tcattgctta tgaaatttag tattcagttg tttttgttta tatgcttttt
     1981 aatatattat tccttcaacg gtgggaacgt aaatccgaac tggtacatac tgcttactcc
     2041 agttctgctt ttattgatgg caggacttgg acttggattt ggaattatag tatcttccct
     2101 gactacaaaa tacagggatt tcagtttctt agtaagtttc ggagttcagc ttttaatgta
     2161 tgcaactccg gttatttatc cattgtctgc tctcccggaa aaatataagt ggatcgtact
     2221 tgctaatccg atgactgcta tcattgatac attcagatat gcatatcttg gtgcaggtac
     2281 tttcaacgca gggaatttaa tttacagctc aatatttatg gttgtaattt tgtcatttgg
     2341 aattgtgata ttcaatagag tcgaaaaaac ttttatggat acagtctaaa tattttataa
     2401 tgagttcagt aataaaagta gaagatcttt caaaattgta ccgacttgga atggtaggca
     2461 gaaggactat agctgaagat ttaaacagat tatttgccag attaacaggt aaagagaatc
     2521 catatctaaa agtcggagaa acaaatacaa gaaataaaaa aggactttct gattatgtat
     2581 gggcactgaa aaatataaat tttgaagttg gtcagggcga gagattcggt atcattggaa
     2641 agaatggtgc aggaaaatct actttgctga aaatactttc cagagtcgca ggacctactt
     2701 caggtaaaat caagatcaag ggaagagtcg caagtctatt agaagttggt acaggatttc
     2761 atccggaact cacaggcaga gataatattt ttttaaacgg agctatactt ggaatgacac
     2821 aggaagagat caaaagtaag tttgacagta tagtagattt ttccggagtg gaaagataca
     2881 tcgatacacc tgtaaaaaga tattcttccg gtatgtatgt cagactggcg tttgctgtag
     2941 ctgcacatct ggagcccgaa attcttattg tcgatgaagt acttgcagtc ggtgatgcag
     3001 aatttcaaaa aaaatgtctt gggaaaatgc atgatgtttc tgaaaaagaa ggaaggacgg
     3061 tgctgtttgt aagtcataat ctgcaggcta tcaggaatct atgtcaaaaa actttgatct
     3121 taaatgacgg agaagttttt acaaatggag aaactgaatt tgccctcaga gaatataatg
     3181 aacttaccag agacattaaa attgatgcaa atactgaaat tggaaataat gtaaacagaa
     3241 gaggaaaagg aagagtcaga tttacaagta ttgaattact cgatgaaagt gggaataaaa
     3301 gatttaaatt tgagctcggt gaaaaaatca gagttaagat cacatatgaa atatttaaag
     3361 aaattgataa tcttattttt gtatttgcat taaattcagg actttcaaga gagtgcctta
     3421 cacttgtaca atttgattta agtgaaaaga aaatgctgcc cggactaaca ggtgaagtta
     3481 tagtcgaaat tcctgaacta aaaatcaagc cgggggaata tcctttgtat tttgaactta
     3541 cagatttcaa tcacaatagt tttacaaaag acgtgataga tgatcttact gcaccgctgg
     3601 aaataattga aaaaaaatcc aaaacagata caggaagacc tacgatatca ggatatttta
     3661 aatctgaagc aagcctgctg aagaatgaaa tttttgaaag taaaaagtta aaaaaagctt
     3721 tataagatat tgtaaaattt gaaaaatcta atttccaaaa tataaagtcc aggttttaaa
     3781 aattgagtaa agaaattgac attggatttt taaacagaac tccgcctcaa tattcaggaa
     3841 gtttaatact taatattctt gggttccaaa ttttcagaac tcttttttat aatttttgga
     3901 gacttagacc ccaaaaaaat aaatataaag aatatacgga aacacttgaa agggatggaa
     3961 ttcttgttat accggatttt ttttcaaatg aacagtttga agaaatcaaa aaagaatatg
     4021 aacaattata ttcagactgg gatccttttg aatttgatcc attgactctg aataacaggc
     4081 agaaaaattt ccccgaatat tttgaaacca ttgctgaaaa atttatatct ccaaacactc
     4141 cttctttttt gaaatatttt ttgaataatg atataataaa tgaattgacg aaagctgtca
     4201 ttcatagaaa aatcagagtt aagccttacc acttattttg gcatttacaa aagagaaatc
     4261 ccgataaaaa tgtagacagt cttcattttg cttcttttcc tcatgctgat gtcccttacc
     4321 cgaccataaa agtatttttg tatctaaggg atacagatga atcaaatgct gcatatgttt
     4381 ttgcaaaagg ttcacataaa ctgacattaa aaagactgat aaatgaatat aagtttagta
     4441 tcagatattc aaaagatgga agtgataatg taacagaatt agaattaaac aaaatgggat
     4501 acagatcaga atctatatgt gggaaagcta atactttatt tatttcaaac aatatgggat
     4561 ttcataacag aggaaaattt acatctatga atccaagagt tacagctcag ttggatttca
     4621 gaaatataga atcatggagg aatactttga acagaggcgg atcaaatatt atttcaagac
     4681 tttcaaaaaa atttgtaaaa tcaattgata ataaaactaa gagtaaaata agtgcaggta
     4741 tcggtaataa tcacatgtca taattatgca cgttaccttt cgagggcaat tcgaagtgca
     4801 ttaaatcaaa gtcttgataa aaaagaatac gagatcatag tagtcgatga cggcagtact
     4861 gatgagactc agaatgttat gaagacttac aatcaaaaga taaagcctat cattctagat
     4921 aaaaattacg gacttgctgc tgcaagaaat gaaggcatta aatctgctct cggacgattt
     4981 gtcattaatc ttgatgctga cgattatatg gatgagaatc ttttacttgt cgaatcatta
     5041 tttttgaatt caaatgccaa ctgggatgca gtaagctgtg attatatttt ggttgacgaa
     5101 aatgaaaaac atttggaaag gatctcaggc aaaaagaagc atattgcctg tggtgtgatg
     5161 tttagaacag atctgttatt cgagattgga ctgtatgatg aaaaatttaa aatgcgggaa
     5221 gatgaagatc tgcggatcag atttgaaaaa aaacacaata tttataatat agaacttcct
     5281 ctttacagat acagaaaaca tgataataat atgaccaata ataaattagg aatgacgaag
     5341 ttcaaaaaag ttttagatac caaacataaa ccccgaaaaa ataaataatt tccaattttg
     5401 aaaaccgttg ccttcatagc agcacgaatg ggttccgaaa gacttccggg taaagtacta
     5461 aaacctttgt caggaattcc atctctagtt catattttta atattctccg tgaagtaaaa
     5521 aatattgatg attttgcagt tgttaccact atacttaaag aagatgatat tatagaaaag
     5581 atctgtaatg aaagtggagt gacatgcatt cgtggatctg ttcatgatgt tttagacaga
     5641 ttcagaacgg catctgaaat tacgaatgca gacgtgatag taagagttac aggagatgat
     5701 cctttgatgg atcctcaaat aattgacaaa gtaatttttg aacataggtc aggagatttc
     5761 gattacactt caaatattga ggacagaact tatccaagag gaatggatac tgaagtaatt
     5821 aattttggtg cactggaaaa atgctggagg gaatccgctg ataaagatga tcatgaacat
     5881 gtgactttat atataagaag aaatcctgaa atgtttaaaa ttaaaagtgt aaaaaaagat
     5941 ggggcacctg cgtctgatct gagattatgt ttagatacag aagaagatta tttacttgcc
     6001 gaaaaaatat atgaaaaact ttatgacggt aaaccaatca gattagatga agtgttagat
     6061 cttttggaga aggaaccatt actcagaaaa ataaatgaaa gtgttttgca gaaaaaagtt
     6121 aaagacaaaa ttttttaata taaataattt ccgtttatta tgataaagct acctgatttt
     6181 aataaagcat atgattatga aaatgaattt tatctttcat gtgataagtc aaggataggc
     6241 aaactgttag ctcattttga actttacaat atgactaaaa gtgccgaagg cgatatagtg
     6301 gaatgcggtg ttttcaaagg agcttcattt gtcaggtttg caatgttcag aaatttattc
     6361 gaagaaaatt ccgaaaaaaa attaattggt tttgattctt ttggtaaatt tccggggacg
     6421 gattttgaag cagataaaaa acttcgtgaa aacctgatca aagatgccgg tgaaaacagt
     6481 atctcgacgg atcagttaat tgatgtttta aaaaacaaag catgcgaaaa aaatgtcgaa
     6541 ttaattgccg gggatatcac tgtaacttta ccagaataca caaagaaaaa tcctgatctg
     6601 aagatctcac ttctgaatct cgacacggac atatatgagc catcagttac aatacttgaa
     6661 gaactatatc caaagatcgt ttcaggtgga atacttgtgc tggatgacta caatgtattt
     6721 ccgggtgaga ctaaagcagt cgatgattat tttaaaggtg taaaaattga aataaagaaa
     6781 ttcccattca gcgccactcc gtgttacatc attaagccat aaccaaaaac ttatatagta
     6841 aattgaattt tctgatttgc ggactgggat ctatcggaag cagacatgcg cgtatactga
     6901 aaagtatagg tgacaataat atatttgctt tcagaacagg aaattcaaaa attaatgaat
     6961 ttgattttca atacactgaa ataaattcat taaacgaact tgataaatat aatattgacg
     7021 gagtactgat aacaaatcca acttctatgc acattgaaac tgcattgagt atagctgcaa
     7081 acggaattcc tatgtttata gaaaaacctc ttgggaatga catgacaagt ttagtagaat
     7141 tacaaaatat tgttagaaca aaaaatattc ctgttctgat cggatataat tttttacatc
     7201 atccggggat cagattaatt aaaaaattaa tttcagaaaa caaaattgga aaagtaattt
     7261 ctgccaggtc ccagttcgga acatatatgc ctgactggca caagtatgaa gattataaaa
     7321 aaagttatgc atcaaattca tcaatgggag gcggagtagt gcttacatct attcatgaac
     7381 aaaattatct gacagacttt tttggaaata tcactgaagt aaaagcaatg aggacaggtg
     7441 aaggaatatt aggaatagac gcagaagaag gtgcagagat acttctaaga catgaatcag
     7501 gaatagtatc aaacatacat cttaattttt atcagaaacc atattacaga aactgccaga
     7561 tcattggaac tgaaggaaca atttactggg attttatgaa accgaatttg acactgcttt
     7621 atcaggacaa gactgaaata ataaagactg gtaaaggtgc atatgaatta cttgatgaaa
     7681 gttacaccga acagatgaag cattttactg aagtgatcaa aggtaatgca aagccggtcg
     7741 cagatctgaa tagaggagtg aaagatcttg aaacagcatt agaaatttta aagcaaataa
     7801 attaaataat tttatgcaat taaaaacaac tgatccgggt aaaataaatt tcaaagaaaa
     7861 attcgaacta aaagataagg taataattat caccggagga atgggactta ttggcagttc
     7921 tttttcggaa gcctgctgcc agtttggggc aaatgtagtg atcgcagaca gggaaggtgc
     7981 tggatctgaa gaacatgcaa agacgttaag tgaaaaattt ggaagagaaa tgacaggtgt
     8041 tactattgat gtggctaaaa gagctgatgt agaaagattg aaaaatacag caattgaaaa
     8101 gtacggcaga atagacggac ttataaattg tcatcagaat aagactgcaa aatttttttt
     8161 aaaatttgaa gactatactg atgaaaactg ggatgcagta gtggaaacta atttgaaagg
     8221 tactttcctg acgtgtcaga tcatcggcgg atacatggca gagaatggac acggctcaat
     8281 aataaatatg ccatctactt attcggtggt cgcaccaaat cacaatctat acaaagatac
     8341 ttctcttgga acaccggcag tttattctgc ttcaaaaggc ggagtaatgg cattgtcaca
     8401 atatcttgca acatactggg caaataaagg agtgagggta aatcagatca cacctcacgg
     8461 agtatggaat aatcatgaag atcagtttga aaagaatttt tcagaaatgt cacctctgca
     8521 gagaatgagt tacaatcacg aagttgcagg tgctgcagtt tatttacttt ctgatgcatc
     8581 gagctatgta acaggtcata atatggtagt ggacggagga tggacgacgt ggtaagagca
     8641 gttaaaaatt aaaaattacg aattgaagtt ttaaatttct taattgtaaa ttttaaacag
     8701 caattttttg caatgagctt ttttcgtttt taaaaaaaca gatatgaaaa ttacagaaat
     8761 attcaataca tacgatcctt taattattaa cagaccttat gtgatcgcag aggcaggagt
     8821 aaatcatgaa ggtgattttg aacttgcgaa gcgtctgata aatgaagctg ctgaaggcgg
     8881 tgctcatgca ataaaatttc agacatataa agctgaaaca cttgcatcaa aagacagtcc
     8941 gtattactgg gatctgaata aagagccgac cgagagtcag tttgagcttt ttaaaaaata
     9001 tgacaagttt ggaaagaaag aatacgaact tttaaaatcc gaatgtgaca aggcagggat
     9061 cgaattcatg tcaacacctt ttgatacagt gtctgctgat ttacttaatg atctgatgtc
     9121 tgtttataaa atttcatctt ctgatattac aaacattcca tttattgaat acatgtgcag
     9181 atttggtaaa ccaattttgc tttctaccgg tgcttctgat acagatgaga taaaaaaagc
     9241 tgtggatgtg attaacagac atggaaataa actttgtctt ttacactgtg tattgaatta
     9301 tcctacactt gatgaaaatg caaatctcgg aatgatactt gatcaaaaaa aaatatttcc
     9361 tgatgcagta ccgggctatt ctgatcatac acttcccgga aatatggatg tacttaaaac
     9421 tgcagtttta cttggtgctg ctgtagttga aaaacatttt acatttgata agacattacc
     9481 tggtaatgat cattatcacg caatggataa agaggatctg aaaaactttt tcagggaaat
     9541 ggaaagagtt tttctgatta caggtgattt taaaaagaaa gcactcggct cagaagatgc
     9601 ggcaataaag aatgccagga gaagtctggt ttcatcaaag aaaataaatg caggtgaagt
     9661 cataagcgaa tctcatctta cctggaaaag accggctgca ggaatcagtc cttcagagat
     9721 aaataaatta atcgggaaaa tagctgctga agaaattgag gaagatacag tgttaaaatg
     9781 ggaaatgttt atatgatgtt aaatgaaacg tctgaaaggt aaaaatataa gattacttag
     9841 attaaaattt aactggaatt ttcagactta tagatcttga caaataaaga tcaaataatt
     9901 gaaaaaggaa atggtcttcc tgttaagatt tatataacat ttggaataat agcaatattg
     9961 tcattaattc cttttctcct tttaaactat tacaatcagc ctacatcaga ggatttctac
    10021 tacagcggag agttattcac attcggattt tcaaaagcat tcagagtact atataaattc
    10081 tggggtggaa ggtattttgg atatttactg atcttagtaa atccactttg ttttaattca
    10141 attgcaggtt acaagttcgc tacatttttt atgatgatac tttattttgt aaccattcat
    10201 ttctttatat caagatttac aagaaacagt cttaatttaa acgagagatt attattaact
    10261 ctttcaatag tatttgtctg tctttactca atgccgtcaa taggtcaggg tttttactgg
    10321 cttatctctg ttctgtttta taatgtcgga attattcttt cctttttatt tttaatagtt
    10381 tattcaaatc agcaaaatgc aatcagcaaa tcaaaaaaat tattatacat gatcccatgt
    10441 gtaattattt taggagcact aattggatcg tgtgaaattg tcggcgggac aatgtttatg
    10501 ttattgactg cgttgctggt aataaatttt atcaaaaaca gatctgtaaa ttattataca
    10561 ttaatatatc tgatcttgtg tttgatcgga gtattgattg tcagctccgc tccaggcaat
    10621 gtctcgagag ctttgcagta tccgggtaat cacgattttt tattttcatt tacaaatgca
    10681 ctttcatttg cttcgctgga aatattgaaa tggacattca taacgccatt gttattagtg
    10741 acatttttat tgctgcctgt ttttaaaaaa cttataaatg ataattcact taatgatgta
    10801 tttaaaataa atccattaat tgcttttgtg ctgtttgcag gattgatggt ggtaaattca
    10861 tttctaatat tttggagtat agcggaagca ccctacgggc ggatcttaaa ttttatttat
    10921 ctgatatttt taatttgctg gttttataac attgtcgtat tcctttatta ctttagaaat
    10981 aaatttaatt tatttactgc aaggattccg aatgcagctt tcggaattgc attagtgctt
    11041 gcactaacat ttggcttaaa agacaataat attaaaactg catattcaga tatgttctca
    11101 ggaaaagcat ccgagtatga taaaaaggtt aatgaaagat atgaacatat ttacaacagc
    11161 aattcagata cagtttatgt taagagtatt ttgaatgttc ctgaatcttt ttttcagcat
    11221 gacatttatg aaaaagctga gactatgtat aatgaaggat atgcaaagca ttttaagaaa
    11281 aatactattt tgttaaaaaa gtaaatttga taaaagataa aaaaatagtt gcatcgatag
    11341 aagcaagaat gacctcaagc agactgcccg ggaaagtact gatggaaatt aacggtaaac
    11401 cggttttgga aattcttatc gacagactga aacgatcaaa gtatataaat gaaattgtga
    11461 tcgctacttc atcgaatgat gcagatgaca gaatcgaaga gctgggaaaa gaattaaatg
    11521 ttgctgtgtt cagaggcagt gaggatgacg tactaggcag agttgtcggc gcagtggaaa
    11581 ctttgaaagg tgatattatc gtcgagatca ccggtgactg tccgctgatg gatccggaag
    11641 taatggatag cgtgattgag gaatatatag aaaattaccc tgaatatgat tatgtaacta
    11701 atatcggata tgtggaaaat gaagtccgtg aaataccgat cggaatggac atcagagttt
    11761 tcacttttaa agatctaaaa tatatcagtg aaattacaga tgatcccgaa gacagggaac
    11821 atgtatcttt atattttttc aggactggta aaaataaata taaacttttt aatataaaga
    11881 ctccggataa atggaaaagg aattatagtg taagattagc tcttgatacg aaagaagatt
    11941 ttcagttcat agaaaaaatt tatactgagt taaataagat aaataaagaa tttgggcttg
    12001 aggatatttt aaatttttta gatgaaaata aggagttgtt aaaaataaat tctgaagtca
    12061 tacaaaagac agtttcgaat ctttgagtaa agtaaataaa tattcagctg caataattgg
    12121 ctgtggagat attggataca ggtttgatgc ggactggtca gacgacaggg aaggtgcatt
    12181 gacacatttc agagcattga aaaattcaga taagtttgaa tttatttctg tagctgaaaa
    12241 aaatcaggaa acacttgatt taataagcag tcattcaaat atttcatgtt atgaaaatta
    12301 tataaatatg ttgagtgaac aaatgccgga tataatttca attgttacaa atgacgaatc
    12361 acattacaaa attctaaatg acattttata tataaaagac tacaaaccaa aatttgtatt
    12421 ctgtgaaaaa cctctagctt tgaatcttga tgaaatagaa tctatttgcg aaaagtatag
    12481 tgaagcaggg attcctcttc aggtaaatta tacgagaagg tttttaaatg aatttataga
    12541 aattaataaa gatattgata aacaaaatac cggcagaata gaatcggtaa ctttttatta
    12601 ttcaagaggt cttgtacaca atgcttctca ttatattgat ctggctttat ggtatttcgg
    12661 aattccggat gaagtgctca gcttttcatc aaagaaggga attactgatt ctgataaatc
    12721 tgtgagtttt attttaaaat ataatgaagg acctgaattg atttttatcg gacttgatac
    12781 aggtaaatta tccttcgctg aaatagatat agtcggtaca gcaggaagga taaagtttaa
    12841 ttttaagaat gagattgaaa gatatgaagt aatatcaaat ccggtattta aaggatacac
    12901 ttcattaaat ttagaaagtt cagtgccggt aaagtttgaa tatgcattac caaacgcata
    12961 tgaaaatata tttgacgtaa ttaataataa acaagaatta cattcacctg cagaaaactc
    13021 aataaaaatt tttgatttaa taaataaaat cagagaaaat agaatatgac aaaattagca
    13081 ttaaccggag gcactccagt ccggactaaa atatttcctc agagtaatac aataggggaa
    13141 gaagaaaaaa aggctgtaat gaaagtatta gattcaggaa atctttctca gtatgttggt
    13201 acatggaatc cggattttta cggtggacca atggtaaagg tatttgaaaa aaactgggca
    13261 gatgcattcg gtgttgacta ctgtatggca gtgaattcaa atacttcggg actgtatgca
    13321 tgcatgggtg catgtgatat tggtccggga gatgaagtga tagtctcgcc atatacaatg
    13381 acagccggag caattgcacc tttgatctat ggcgctgtac ctatatttgc tgatattgat
    13441 gaagacacat tttgtttaga tccgaaaagc attgaagcaa atattactcc aaggactaaa
    13501 gctatattac ttgttcatat cttaggacat ccttcagaca tggatgagat aatggcaatc
    13561 gcaaaaaaac ataatctgaa agtaatagaa gactgcgctc agtcgcctat gtcaacttac
    13621 aaaggaaaat tggttggtac tattggtgat gtcggagtat tcagcctgaa ttatcataag
    13681 cacattcaca caggtgaagg cggaatgata gtcacaaatg atccggtaat tgccgaaaag
    13741 attcagttga tcagaaatca cggtgaagca gtagtcgcag gaaagaagac aaaagatata
    13801 aaaaatattt tcggattcaa ttacagaatg acagaaatgg aagcagcagt cggaacagag
    13861 cagttgaaaa aacttccagg tctgattgaa aaaagaattg agtacgcgga ttatctgtca
    13921 ggtattatcg gtaagcttcc cggcattaca ccgccattag taaaggaagg atgcaagcat
    13981 gtatattatt tgcaaacatt taaattcaat aaagagatta ttggaataca caggaatact
    14041 tttgtagatg cattgagagc agaacttcca actatggtac tcagagaaaa ttcaccaatc
    14101 atcggttgcg ggtttgcagc accgttgtat ttgcaaccat tatatcagca gagactgaat
    14161 cagtgctctt ttaattgtcc cagatatgaa ggtaatgttt catatgaaaa aggaatttgt
    14221 ccagttactg aaagaatgtt ttacgatgaa gtctttactc acgaattcat gagaccaagt
    14281 ttttcaaaag ccgatcttga agatgtagtg aatgcatatc aaaaagtata tgagaatgta
    14341 aatgaattaa aagaatacga atctaaaaat tccgtcaaag tttaaatgtc aaaaaagaat
    14401 attctgataa tatttgtaga tccgggaggc ggcttggtag tttctgaact tgctaagaag
    14461 ctagatgcag atccggaata taatttgaat gtttactgtg gaaatttaag taaagtaatt
    14521 tcggaaaatg caggtttgaa ttttaagatc attagtgaca tgatcaacat tgagactgca
    14581 gaagaaattg taaatactgt ttcacctgat atgatcttaa catctaccgg aagaggcaat
    14641 acagaacagc tttttcgaaa tattgcttac agagacagta ttccaagtgt tgtgattctt
    14701 gatttctgga aagactacag gagaagatgg ctctatgctg atcacaatat tccagaatta
    14761 aaagatatag tttgcgtaat ggatgaactt acaaaaaagg aaatgacaga tgaatatttt
    14821 cctgaaagaa atatcagagt gacaggacat ccttatcttg acaagatatt taaaagtaag
    14881 agagatttaa ttgagaagga aattaaaaat gattttaata ttctttttct ttctcagcca
    14941 ttggacatta taggaataca taattatgaa attcacccgt tcagaaaatt aataaatgct
    15001 gtttctgaat attcggagaa atcagatctt aaaataaatt taaagataaa acttcaccca
    15061 tcagaaaaaa tgtcagagga tctgtcaaag atattggaag aaaaagaaat aaaaaaagtt
    15121 acggtgatac atgtaaacaa tgaagctgta catgagctaa tagagcaagc cgatctgata
    15181 attggttata attcaattgc attatttgaa gcgaggtcga tgggtaaaag agcagtgagt
    15241 ttgaaattat ttcctattaa tgactcgctt atgaaagctt ttgaaaatgc cggcatagaa
    15301 tcctcagatg caaattcgga aagtttattt aatcttttgg atgaaaaaaa agaaacgtat
    15361 atatctgata atatatttga aggtggaatt aaaaattgta tcagagtaat atcagaggaa
    15421 ctgaaagtga aagaaaaagt aagttaaaaa ttaataaaat gatcaatcca tttttaatag
    15481 gaaataaaat atatttgtca cagttatctc aaaatgatat ttcagacagg tatataaact
    15541 ggcttaatga cgaagaagta tgcagggata attctcatgc ccgatttcca aatacgagat
    15601 caaagacatt atcatatata gaacatcttg aaaaaagcaa agatgaaact gcatttgcga
    15661 tccggtggaa aaaaaatgat cttcatattg gaaatgcagc aatacaaaaa attgacagaa
    15721 taaacagaag cgcggaactt gcaattttga taggagataa aaaatactgg ggaaagggaa
    15781 tagcgagtga agtatatgag ctgttaataa aatacggttt tgatactttg aatttgaaca
    15841 gaatttcttc aggacaaaca attgcaaaca aaggcatgat aaaagtttgt gaaagatcag
    15901 gaatgaaaaa agaaggaatt ttgcgtcagg ttttatataa gcaaggagag tatcttgatg
    15961 ctgcagtata ttcaatttta aaaagggaat ttgaaaaagt taaaaagcat ataaaaaaca
    16021 aatgagtgaa ataaaattaa acgagcccag aatgacggga gtgcagtatt gtataagatg
    16081 ctgcatacct cagactcagg aaggtgtcaa gtttgatgaa atgggaattt gtcaggcatg
    16141 tcagtcttca gaacaaaaga tccatattga ctgggtagaa aaggaaaagc agcttcgtga
    16201 aatacttgat aaagcaaaag atttgtctgg aaataattat gactgtatag tgcctataag
    16261 tggtggaaag gacagtacat ttcagttgca cgtattaacg aaaatttaca aaatgaaacc
    16321 tcttgcagtt actttctcac ataattggtt tagtgaaacc ggatggtata atcttttgaa
    16381 ttcacttgag aaatttaatg tagatcatat aatgttcaca ccaaaccgtg atcttgtaaa
    16441 cagattggcg aagaagtcac tggaagccat tggtgatacg tgctggcatt gtcacgctgg
    16501 agtgcctgca tttccattgc aagctgctgt aagatttaat atacctctac tgatatttgg
    16561 agagtctata gctgaatctt caggaagatc ttcatacaaa aatcctttga tggaattcaa
    16621 tgtagattat tttatgaaag tatcgagaaa gagagatcct caggatatgg tgtgtgatta
    16681 tatttcaatg aaagatatga aaccattttt ttacccttcg gtgcaagagc tggaagaatc
    16741 aggagttagg ctgatgcacc ttggagatta tattttttgg gatgatgaaa gacaaatgga
    16801 atttgtaaga gatacttatg gctggaaaga gactgagatt gaagggacat acaaaggtta
    16861 taaaagtgct gaatgtataa tgcccggaat gcacgacttt acaaattatc ttaagagagg
    16921 atatggcaga tctacttatc aggcaagtgt agatgtaagg aacggtctgc tatcacgtga
    16981 agaagggttt gagcttgcaa ataaatacga tatggaaata ccggaatcac tggaatatta
    17041 tctgaaaata accggaatga gtaaagaaga attttataaa ttgatgaaag aaaagagatt
    17101 agatgaaatt aaaaatatcg aacttccggt aattcaaaag acacgaaaga ataaagaaag
    17161 gatattgcct cttgctcagc aattaataga agaggaagag ttaaggagag caaataaata
    17221 atatggaaac atcagtcata gaaaagaata tttttcttca aaagagcatt tctgaaatta
    17281 ctgaatcttt acggaataat cttatcactc cattggaaat tgcagaagca tgtataaaga
    17341 ggatagagga atataatgaa aaatataaag tatggataat atatgataaa aaaattctat
    17401 ttgatcaggc agaaaaaatt actgagagat taaataccgg atgtaatata agaaaacttg
    17461 aaggaattcc tgttggaata aaagatatat ttaatacata tgattttcct acgcaaatgg
    17521 gaagtccctt atggcaaaat tttacatcag gaaatgattc gagaattata ttttatctga
    17581 aacaacaggg atcgataatt ccaggaaaaa ctgatacagc agaatttgca gttcatgaaa
    17641 ttggaaactg tctcaatcca catgatatta caagaactcc aggaacttca tcaagcggat
    17701 ctgcagtagc agttgcactt ggtatggttc cggcagcgat tgggactcag acagccggct
    17761 caatagtacg cccagctagt ttttgtggag tatatggatg caaaccttct ttcggactta
    17821 ttcccagaac tggtatgctt aagacaactg acagtcttga tacggtagga ttttttacat
    17881 caaggtatga agatcttgaa actgtatttc atgtcgtaag agttcacggt tcaaattatc
    17941 cttttagcca taatgcatta actgatgagc aaagacaaaa caaaccgtca gaaagaaaat
    18001 ggagagttgc gtttgcgaaa acacatacct gggatttcgc acctgaatat gcaaagaagg
    18061 caattgaaga actggttaat aaaatttcat taactgatga tattgaaata gaagaagcaa
    18121 aacttcctga aattacaaaa cagtctcatg aggttcatca aatgatatat gataaaacac
    18181 tttcatatta ctttcaggaa gaatttaaaa aacctgaatt tatatctcct gtaatgaacg
    18241 agatctttga acggggaaat aaaatcacag tagatcaata tcatacttct ttaaaggatc
    18301 aggaaaaaat agcttttgct atggaagaat attttaaaga tgttgacatc gtaatatctt
    18361 taagttcggc aggggctgca ccattaagaa atgaaaatga aaagcctgac ccttctttga
    18421 tgtggacatt aagccattta cctgttatta gttgtccggt atttgtttcg cctgacggac
    18481 ttccatttgg aattcagatt actgcaagaa aatataatga tctgttgtta ttcagattta
    18541 cagatcacat caggaaaaac ggacttatcc ctgaaggtgt aaatccaatt ttataaatac
    18601 tacaaattta aaaagaaaac aagttaaaga aattttgaat ccaatttcaa ttgtaaaaag
    18661 cagactgttc agactgaagt tcattaaaga tctaaaccgg aaaaaaaaga tcaataaatt
    18721 atacagtaaa ggagtgaatt caggtaagaa ttcagaaaaa gtattattct ggactacagg
    18781 tggaatgatt gtacagacaa atatagaagg gatgctcgct gcttcattaa agctcagagg
    18841 aaataaagtt catgtggttt tatgtgatgg agtatataaa gcatgtgcaa aaagagttga
    18901 tcatcctgac ctgcctgtga aagaatgggg aaaattttgc aaatcatgta taagacaaaa
    18961 ctctgctgta ctggataatc ttggagtaga gtattcatat gtcagtgagc atataacacc
    19021 cgagagaatt tatgagttac gtaaaacatc attggaaact gatctgacga attataaatc
    19081 gatttcttta aatggttatg ctcttggtaa taatcttgaa tcagcaatga tacgacatac
    19141 aaagagtagt aactttattg gtcaggaaga aattttgaaa gaatattctt ttacagttct
    19201 tgtagttgcc gaggcttcat caaatattct gaaaaaattt aatccggata aagtatttat
    19261 gtctcatgga atttatgctg actggggtcc ggctttaact acatcactta gcaaaaatat
    19321 cacaataaat tcatatattt gcagttatct aaatgtgcat tttcaatttg gaactgttaa
    19381 aaaaatgaat gaaacttttt taacgttgca tgataatgcc tggaatataa tcaaagcgga
    19441 aaaattaaat gaaaatcata aaagaaaatt gtcaaagttt ctttccggaa gatataaaga
    19501 aaatattgct catgatatgc ggggtttact aaaagaatac cggggtgata cagaacattt
    19561 atattcaaaa tataacctgg ataaaaataa acctgtgtgg ggaataatga ctcatataaa
    19621 ctgggatgca gtctcagatt attttccaat gatttataaa aattttgatg aatggctggg
    19681 tgaaactata aatattatta aagaagtaac tgaggtgcaa tggttaataa agattcatcc
    19741 atctgaactc aacgataatc cagatacagg ttgtcaaaaa tatattgaaa agaattttcc
    19801 cgatcttcct gatcacataa aagtaattac tatggatgat gatatcagtc ctttagattt
    19861 ttataattta ttatcaggag gagtaactgt cctggggacg ggcggtctgg agctttcact
    19921 tatgggaaaa ccggtaatac ttgcaggtga ggcacactat tcaggaaaag gatttactta
    19981 tgacggaatt gataaaaata aatatgcaga attattagaa aacacttcag agataaagcc
    20041 gcttagcaaa gaaatgcatg aactggcaat gaagtatgct tatacttatt ttatcagaaa
    20101 gcaaattcca atgcctccta caataaaaga tgatctgcta atagatttta caaagcttga
    20161 acatcttttg cctggaaaaa ataagcatgt ggattttctg tgtgaaagaa ttttagattc
    20221 tgaagatttt atactgccgg aaaatcttgt ttcaacttta acggatagta aacctaatta
    20281 aaaggatttt tggtaaagga atatctgaaa atagatattt gttgaactgt gaattttctt
    20341 aaaattataa atttacaatt ataatttcac atctccaaaa gctcaaataa aagtttgtca
    20401 aaattttctg atataataaa ctgatgaaga tctcagtaat cgttaccacg tttaacagag
    20461 cgaagatcat cccaatgtgc ctggattcgt tagttgtaca gaattacgaa aaatctgatt
    20521 ttgaaattat tatagttaac aataactcaa gtgataatac agaagaagtt atagaagatt
    20581 acattgaaag gtatcctgag ataaatctaa aatattattt tgttccgaga ccagggcagg
    20641 tttatgcaag acaaattgga attcttgcgg caagcaatga aatacttact ttcactgatg
    20701 atgacggaat tctcgtaaag gactggctta aagaaattgc aaatgtgttt agtatgtcag
    20761 atgaagtagt aggagtagct ggcaagatca agatcaaatg ggatgaagat ccgcctgaat
    20821 ggatatttga ttatgagaga cagcttggga aattagatta tggaaaagat attaaatttg
    20881 aagttggtct ttatatgaat gcaggtaatc tcagtattaa aagggatgta ctttttgaag
    20941 ttggtggttt taatccggag atggttggag aatggctgat gggtgacggt gaaaccgggc
    21001 tttggaaaaa attattcaaa aaaaaatatc ttattggatg ggctccggac gcagtaatgt
    21061 atcattacca ggtagcaaaa aaaaatgcta cagttgaaga tattgaaaga agattttata
    21121 ataatggtat ttgtattcca tataatattt atgcaattga tcaggaagga aaaaaagcat
    21181 tacttaaaaa tatgttctat gcctctaagc aatgggtcag atggattatt aaaaaaaacc
    21241 gatttggaat tttagataat aagaaaagcg aatattatgc aaagtttaga agtgctttct
    21301 attttgctca gatcagatac acactgaata tactttttag cagagagttt agaaataaat
    21361 tattaagtga cgactggaaa ataaatttaa ataatacagg aaaaccatta tgaaaaaagt
    21421 atatgttggg atgagtgcag atctgataca cccgggacat ttaaatataa taaatgaagc
    21481 caaaaagtta ggtgaggtaa cagttggtct tcttactgac aaagctatag cgagttataa
    21541 aagacttccg gcacttacat ttgatcagag agaaattatc ataaagagta ttaaaggagt
    21601 tgaaaatgta ataccgcagg agactctaga ttatgttcct aatcttcaaa agctgaaacc
    21661 tgatttcgta gttcacggtg atgactggaa agacggtgtg caaaagcaaa ccaggcagag
    21721 agttatagac gcattgaaag aatggggagg ggagctggta gagataaaat atactgaagg
    21781 gatctcatcc acacagctta atcaaactct taaagatgtc ggcactacac cggatgtcag
    21841 gatgaaaaaa cttaagagat taatgggagt aaaacccctt cttagaatta ttgaagctca
    21901 taacggaatt accggactta ttgtcgaaaa tattttttca aaaagagatg gagtgaaagt
    21961 tgaattcgat ggtatgtggc ttagcagtct tacggattct actgcaaaag gaaaaccaga
    22021 tacagaaact gttgatgtca ctgcaagact tcatacactt aatgatatta tagaagttac
    22081 tacaaagccg atcatttatg atggagatac aggtggcagg acagagcatt ttgcatttac
    22141 cgtaagaaca ttagaacggc tcggtgtttc cgctgttata attgaagaca aaaccggctt
    22201 aaagaaaaat tccctttttg gaactgatgt agatcagact caggaatcga tagaaaattt
    22261 ttgtgaaaaa ataaatacgg gaaaaagatc aagagtcacg gaagatttta tgatcatagc
    22321 aaggatagaa agtcttattc tgaaggcagg tcttgaggat gctttacaac gagctgaggc
    22381 ttatatcaat gcaggtgcgg acggaattat gattcacagt aaggaaaaat cacctgatga
    22441 aatattggaa ttctgcagag agtatgaaaa atttgaaagt aaagtgccgc tgattgcagt
    22501 accttcaagc tacagttcta tctatgaatc tgaacttgaa aaagcgggag tgagtatcgt
    22561 aatttatgca aaccatcttt taagaagtgc atatcctgca atgattgaaa ctgcaaaatc
    22621 aatattggag aatggaagat cttatgaaac agataacaat atgatgtcaa ttaatgagat
    22681 tttaaattta atccccggag gaaaataata attgaagccg gcagattttt tcaagttact
    22741 ttctgaaaac aaaatacatt tttatacagg agttccggat tcacttctta agaacttctg
    22801 ttcttttgta aatgataatg tatctcctga aaatcatatc attagtacaa acgaaggatc
    22861 tgctgtcgca ctggcagcag ggcattatct ttccacagga aacccgggtc tagtttacat
    22921 gcagaattcc ggattcggta atgcaataaa tcctttgctg tcactttctg atactgaggt
    22981 ttatggaatt cctgtaatgt taatgatcgg ttggagaggc gagcccggaa agaaggatga
    23041 gcctcaacat ataaagcagg gaagggtgat gatcgaattg ctgaaaagtc tggagatacc
    23101 tttcagaatt tttagttctg aaaataacga agtgaaaaaa gatattaaag aattgtacga
    23161 aatttcaata tcaaataact gtcctgcagc aatgctgata tcggaaggat tttttgaaag
    23221 ttataaatca gaaaataatg tcgaaaataa ttatcctgtt agcagagagg aagcaattaa
    23281 attaattgcc ggacttacaa atgaagatga cataataatt tctacaacgg ggaagacttc
    23341 acgtgagctg tatgaattca gagatgaaaa aatctttggt catcaaaatg attttcttac
    23401 agtcggatcg atgggacaca gttcacaaat agcattagga atttctttaa ataaaaaaaa
    23461 taataatgta tttatactcg acggagacgg agcggtatta atgcatatgg gatcattagc
    23521 tctcatagga tctgaagcac ctgaaaatct gaaacacata attctaaata acggagcgca
    23581 tgaatctgtt ggtggacagc caacagcagg gtttaagata agttttacag aaattgcaaa
    23641 agcctgcggc tataaaacta ctttgcttgc agaaacaagg gaacaaataa aaaatgattt
    23701 taacgtacta aaaaattctt tcggtccttc acttcttgag atcagaataa ataaaaattc
    23761 aagatcagat cttggtagac cttcatcatc gccaatggaa aatagaaatg atttcatgaa
    23821 gtttctgaat cagctttagt atgacacaaa aagtaattat agaaaatggc agtattaaaa
    23881 agttaactga tctgattgat attcagattg taaaaaggat atttcttgtc actggaaaaa
    23941 attcatattc aatttcagga gcggagagaa aactaaaaat acttgacaga aattttgaga
    24001 cagtacactt tgatgaagtt ggtgaaattc cggaaattga aaatgtaaaa aaagggatag
    24061 aattatttaa tgaatctgat tctgatatag taattgcagt aggtggtgga aatgttatag
    24121 atattgctaa atcaattaat attctatcca ctcattctga aactgctgaa gatctgatta
    24181 tgggtaagaa aaatatttca atcaagggta aagacttaat tgctattcca acaacagcag
    24241 gatccggaag tgagtctaca cattttgcag tagtgtattc aggtattgat aaatattcaa
    24301 tggcagacga atttatactt cctgatattg caattgtcga tccggaactt acatacagcc
    24361 ttgacagcag acagactgct atatcaggta ttgatgcatt ttgtcaggca gtagaatctt
    24421 tctggtcagt aaattccact aaagagtctc gtgaatattc taaggaatct ataattttaa
    24481 ttttgaaaaa tctgaagaaa tgtgtaaaag atccgcacga aagtgcaagg tttaatatgt
    24541 gtatggcatc aaatctggca ggtagggcga tcaacatttc aaagacaact gcgccgcatg
    24601 ctgtttcata tacactgact tcagaatacg gaattcctca cggacaggcg gtttgtgtaa
    24661 cgctgccagg gtttttggaa ttcaattata atttaaatga tgaaaatatt tctgaaggta
    24721 ttaataagga agaacataaa gtgctaatgg atgaactttc aaaaattctt ggatttaaaa
    24781 gtaattctga tgctaaaaag tttttgatcg aatttattga cagtcttggt ttgaaaataa
    24841 atctgactga cctggggatc aaatcttctg aggatataaa tatcatttct aagaatgtga
    24901 atacagaaag attaaaaaat aatccaagaa aaatatcaga gtccgattta gaaaaaatct
    24961 tatctgatat tctttagtga ataaaggtaa tggaaaaggg acaagccggt tcaaaataaa
    25021 atcaggtatg gtctttgata aagtaaaagg gaaaaagata ttaatattag atattgaaga
    25081 actttccttt atcccgtata tctttccgat ctatgaatca ttgaaaaaaa aaactaatct
    25141 aatttctttt tatatctcaa ctttttatcc gggtgctgag gaacttaatg tttttgaaat
    25201 tcctctgaat aagcaatatc atgtgaaatt caattctgaa ttgtctgaag cagatatttt
    25261 tttatcacca cacatatatg gtattgggaa tgaaagaact ttaaggatac atataaacca
    25321 taatcagccg gtgaagtatc agtcatatat gaaaaatgat tttaaaaatt ttgatatgca
    25381 ttttcttaca agtcctttgc acagagaaca aacagaaaat acgataagag attattcgct
    25441 tgaagaaatg aatatcaaat tatttgatat tggatatgca aaatctgacc aacttttgaa
    25501 cggagctttt aatcgggaaa atgttttgaa tgaccttggt ctggatccga atagaaaaac
    25561 tattctttat gcaccttcat gggatgaagg tctttcacta aggatgaaag gtgaaaatgt
    25621 aattgaagag ttattgaaaa tcaacaatgt aaatgtgatt gtaaaacttc acccaataag
    25681 ttattgtcct gcagacggac caaactacga attttataca ggaggaatca actggaaaga
    25741 gcggctggaa aaatttgatt cgaatgaaaa tttcaagaat gtgacaacag gaaaagctga
    25801 ccccttactt gcagcagcag acgtgatggt tactgatctg agcagtgttg cgctggagtt
    25861 tattttgctc gacaagccgg tgatatatat tgattgtcct gaattttttg agagaacgat
    25921 tgaaaaaact tatagcggat ttggaaatac aactgcagag tttgttaaaa atgatcctaa
    25981 agcaaatgct ggaagacata caggtattgt tattgaaaat gtttcagaac ttcaaatggc
    26041 agttgacaga gcattaaaag atccatttga actttcgaat aaaagaaaag agcttgcaaa
    26101 aaagctgact tacaatccgg gcagagctgc tgataccgct gctgaaaaaa tacttgagct
    26161 gcttgattta aactgaaaat accctgcaga tatttttaaa agataatgaa aataaaatta
    26221 aatagttttt aatataagtt tgaaaaagga aatagaaaaa tattttgaca gactctggcc
    26281 gatatgcaga agtctgactg gtgacggaaa cagggagact ttaaaaattt tatctgaaat
    26341 agcagatatt aaagtaactg aagttccttc aggcacaaaa tgttttgact ggaatgtacc
    26401 acctgaatgg aatgtcaaag atgcatggat aaaggacagt aacggaaaga agattattga
    26461 tttcaaaaaa aacaatcttc atttacttgg atattcagaa aagtttaatg ggaaattaaa
    26521 tttagatgaa cttaaaaatc atttacatac tttgcctgat cagccgaatg taattccata
    26581 tctgacttcg tactacagtt cccgatgggg tttttgtctg acacataaac aattcaaagg
    26641 attgaagaaa ggtgagtatg aagtttttat tgatgtgaag aaagatccaa aaggttctat
    26701 gactgtggga gaagctgtaa tacccggtca atccaaaaaa gaaatactgc tatcgactta
    26761 tatttgtcat ccatcaatgg ctaataatga attatcagga cccttagttt cagcttttct
    26821 ttttaaagaa ttaaaaaaga agaaggacag aagatataca tatagatttt tattcttacc
    26881 cgaaacgata ggtccgatct ggtatttatc aaaatatgga aacgatctta agaaaaaact
    26941 tcaggcagga tatgtactta cgtgtattgg tgataaagga aatttcactt ataaaaaaag
    27001 cagagcagga aatacacttg ctgacagagc tgcagagctt acattgagtc agaatgaaaa
    27061 aaaatatttg attgaagatt tttttccaac aggaagtgat gaaaggcaat actgctcacc
    27121 gggatttgat ttacctgtag gatctttgat gcgaacccga tatggaaaat acaaagagta
    27181 tcatacttct tcggataata aagatttcat ttcttttgac aaaattgaaa agtcagtcaa
    27241 tatgtacatg gaaattttat atgtacttga aggcaacaga tattatttga atacaaaacc
    27301 atattgtgaa ccgcagcttg gaaaaagagg attgtatcct acacttggct cacagaaagc
    27361 aacaacagaa tttgtaaatg caatgatgtg ggtacttaat ttatctgatg gagcaaatga
    27421 tttgatttca atcagtatca ggagcggatt aaaatttaac gttatagaag aagcagcaag
    27481 aagattacat gaaaaaggac ttttgaaaat atatttaaaa tagtaaaata ttgcccgaaa
    27541 gaaaaaataa ttatcggtca aaaagatatc tgattaccgg attttcagga tttgtcagca
    27601 gaaattttat tgagtatttg aattccaaaa atgaagattt tgaaatactg gggattgata
    27661 taaaaattcc tgactttgaa tatgaatttt ataataattt aaaaataaat tttcaaaaga
    27721 ctgatctacg ggaaagagaa aaactgtgca aacttgttta tgattttcag ccggattata
    27781 tactgcacct tgcttcgtat agcagtgtag ccggaagttg ggaaaatccg gcaaacagtt
    27841 tccagaataa tttaaatata tttttaaatc ttcttgaggt ggtaaagaca ataagaagta
    27901 tgtgcagaat tattttggta ggctcatctg aagaatatgg agatgtaaaa atctcagatc
    27961 tgccgctagt agaagagaag attccaaatc catccagtcc atttggcata gccagattgt
    28021 cacaggagat gcttgcaaaa ttatatgtag aagttcacgg acttgacatt atactgacca
    28081 gatcatttaa tcatattggc gcgggtcagg gagaaaactt tgctgttgct tcatttacaa
    28141 agcagctagt tgaacttaaa tttcacagca atggagattt tgtaattgca ggtgacgttt
    28201 caattgtcag agatttcatt gatgtaaggg atgtagtccg agcatattac cttctgtttc
    28261 aggaaggcag gaaaggtgaa atatacaatg tttgcagtgg ttacggaata tcgcttcaga
    28321 atattataaa tataatgagt gatatattaa atttaaatat tacaatcaaa gaagacataa
    28381 gtctaagaag accaaaggat aattcggtaa ttataggttc aaataaaaaa attaaatctg
    28441 agatcggttg ggaaaatgag atctcacttg aggatagtct ttctaacatg ataaattatt
    28501 atttgcatta ttcagaaata tctgcttcaa ataaattgcc aacattatga gtacagtaat
    28561 aaaagtagaa gacatatcga aattatataa acttggtata gtatcaagca agtctatagt
    28621 tcaggatgta aacagattta tcgcgaggat aacaaataag gaagatcctt tttcaaaatt
    28681 aggtgaaata aatgacagaa ctgtaaaaag gaattcggat tatgtctggg ctttgaaaga
    28741 tataaacttt gaagtgaaag acggagagat acttggtatt atagggaaaa atggcgcggg
    28801 gaaatcaaca ttactgaaaa ttctttcgag agtgacatct ccaactaaag gaaatataaa
    28861 aataaaagga agagtggcat cgcttcttga ggtcggtaca ggttttcatc aggaattgac
    28921 tggaagagaa aatatttttc ttaatggatc aattctcgga atgaccagag ctgagatcaa
    28981 aagtaagttt gaagagatag tggaattctc aggcattggc agttacattg atactcctgt
    29041 aaagaggtac tcatcgggaa tgtatgtcag gcttgcattt gcagtcgcag ctcatctgga
    29101 tcccgagatt ttaatagtag atgaagtcct tgcagtaggt gatgctgaat ttcaaaaaaa
    29161 atgtcttggt aaaatgcaga atgtttctca aaacgaagga cgaacagttt tatttgtaag
    29221 tcacaatatg gcaatcatgt caaagctatg caacaatgta attgtcttaa aaaacggaca
    29281 ggttgatttt ccaaaaggtc catcggatga agggattcaa cattacatat tagaaagaaa
    29341 ggaaaaagct gagatcaatt tatcgaaaag gacagacaga accggagaag gtaaagtgag
    29401 attaacagac tttgctatga tgaatgacag gaatgagaaa attgatattg caatttcggg
    29461 acaaaaagta aaattcagaa tacattatga atgtattgaa aatccagacc ctagaaatat
    29521 aactgttgct attgcggtgt caagatcaga tggtttttta gtaactgtac ttggaaatga
    29581 tatttcatcg agaggttttg attcaatttc gcaggaaggg tattttacat gtgtactgaa
    29641 taaacttcct attacatccg gctcatatct tttgaattta atagtgtatc aaaatggtac
    29701 aagacaggac tggatccagg aagctgttac tattactgtt gaagacggag atttttataa
    29761 caccggaaaa attgtaccat ctactcacag aagtgtattg cttgagcatg aatggtcttt
    29821 aaatgattaa ggaattaaaa attaaatgaa aaaaatagta ctggggtttt ttgctttgtt
    29881 tggaatagga ctttataaga ttaaaaaaga agttgttaaa aaaccaaagt ctgaatacaa
    29941 accgattaaa aattttgaag ttacatctgc ctttgagcat aatactgcag aaagaatgaa
    30001 tgattttttt tcagatcctg aacatgtaga aaattatatc agtgatgtca gagttgattt
    30061 ttataaacag ataactgaat attttaatga aaataaaatt gatctgaata ataaagaagt
    30121 attggatgta ggttgtggta caggtcatct tctgatgtac ttatctgatc attacaaaat
    30181 gaaaaaagtt tatggaattg atttttcaga tgcggcattg gcagttgcca aaaagaattt
    30241 acctgaagga gaatttatgg tgcatgatat ttataaagat catgataaaa aatatgacat
    30301 tgtattatgc acagaagtac tggaacacct tgtagatcct gaagatgcac tttcaaatct
    30361 tgaaaagatg atgaatgaaa attctttgtt atttattaca acgcctaatg gaaggacaga
    30421 tacttacaaa ggtcatatta attactgggg tcctgagggt tgggaaatat ttttgagaaa
    30481 aaactgttca cagaaaaatg taaaaacagg tcttcttgta gactccagat ttaactacgc
    30541 ctgtattaaa aatgcagaat gaaattttta aattgttttg aaaaagcatt ccagaaaaat
    30601 tctcatactt tcatacagtg atctgagtaa agatccgaga gttttaagac agatcaatgc
    30661 tttaaaggag aaaaattcaa tcactgcatg tggatataaa ccttcggaca ttgacggcat
    30721 aagatttttt tctatatatc caaaaaatga aagtcttgca a
//
LOCUS       DAORVQ010000017        29361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_538_length_29361_cov_5.307719, whole genome shotgun sequence.
ACCESSION   DAORVQ010000017 DAORVQ010000000
VERSION     DAORVQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 29361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29361
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_538_length_29361_cov_5.307719"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PLX80_02765"
     CDS             <1..1250
                     /locus_tag="PLX80_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY19750.1"
                     /translation="SILFGCHYLTYKTFKNFLISKFPGNRKLKYIYGYPFIIFNLPYL
                     YVIYNLLTSTKTPYWIINYGYIPFYIFQGAVLFIGLYLIIGKLIKAPFTIPYYFLNKI
                     KYFKERFNSFFAKKSIKKLDSSRRAFIKTSAAFVSGYAFIGATTGVLSKNDYQIERVD
                     LSIPGLPEELKGTTITFISDIHSGPYMNENLMREYAEVINDLKSDMILMAGDLTNSQK
                     EEVFPFVHAFKVLKAQHGIYSSLGNHDYFSDANFISKVVNSESPVKILRNQSEIISIK
                     GKDICIMGIEDTRQSGSAPDNILINYLDETIIGTKKLFSDKNLNYDETAKIALFHKPY
                     FFSELQDRKIDLFLSGHTHGGQVVLADFGNINISFAGAVSDFIKGLYKNPTSQMYVSR
                     GIGCVALPLRLNCPPEITVFRLV"
     gene            complement(1247..2233)
                     /locus_tag="PLX80_02770"
     CDS             complement(1247..2233)
                     /locus_tag="PLX80_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY19751.1"
                     /translation="MKKLFKILGILLGLVVVLLIAGFVYIKTSLPDVGPAPEMKIVST
                     PEMIERGEYLANGLASCIDCHSKRDYSKYAGPMIEGTAGMGGENFGEGAGTVLAKNIT
                     SDKETGLGNWTDGEIFRAITMGVDKDGEPLAPMMPFMYYRNMDEEDIKAIIAYIRTLP
                     AVKNEIPEHSLNFPVNLIFRTLPQKPDFKNRPETSDPVALGQYYSGGCMACHSPMEKG
                     EFIEDKMFSGGVEFPNPKGGIIRTSNITPDKETGIGNWTKEQFIKRFNSFSDSNYTAA
                     NIHQGEFNTIMPWTFYSEVSEEDLGAIFDFLMAQKPISNKVVKFDPNGIPAY"
     gene            2323..2919
                     /gene="rdgB"
                     /locus_tag="PLX80_02775"
     CDS             2323..2919
                     /gene="rdgB"
                     /locus_tag="PLX80_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQY19752.1"
                     /translation="MKKILIASGNSHKIKEISSIVSDIPNLEVTTLNNFGICLEVEEN
                     GNTLEQNALIKAKSVYSLLGMPVISDDTGLFVNALNGEPGIYSARYAGLNAAYKDNCD
                     KLLDNLKEIDDDNLQAEFKCFICLLINPEEFYFFEGICRGRICRSPKGENGFGYDPVF
                     MPDGFEKTFAELTDDEKNLISHRGRALEKLKGFLINFQ"
     gene            complement(2906..4327)
                     /locus_tag="PLX80_02780"
     CDS             complement(2906..4327)
                     /locus_tag="PLX80_02780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQY19753.1"
                     /translation="MNKNFKKIFSALFSLLIFVSGFIYLNLSKKDISEPEIQMSPKKF
                     MYNDKFGKADKYTSKDISRKKELTNIRINELPGASEPVIKCSPTSNQILIVSANDFSI
                     DENKARIFISVNKGLDWKAGEIDLSECYSASSYSDPWIEYDNNSELFFTSIQYDMHKN
                     INEGIFFARSNDNGINWTSDKNLLASNSTSSVKIDKPKIFISKNDIIYIYWTEISGYK
                     SHINLIMSKDKGKSFSDPIALTDDKSHFSSMIEDNKGNIYLAYVNDGKSIKVIRSFDE
                     GNEWSNIKSYLKINAAGKSVENNSILKSVKSSGIRINSEPSLELSSEGEILITYSARG
                     IGNDLSDVYFAKLINENSEFTIPARINSDETENDQYQPLIETDENGLIYIFYQDSRND
                     NNNILTESYISVSSDGGNTFTDKNLSTNPYSPSEIAVGKYFGDYNSFIVSGKDIIAVW
                     TDGRNGNFDVYAGILNRDHIIGN"
     gene            complement(4336..4407)
                     /locus_tag="PLX80_02785"
     tRNA            complement(4336..4407)
                     /locus_tag="PLX80_02785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4372..4374),aa:Arg,seq:acg)
     gene            4545..5189
                     /locus_tag="PLX80_02790"
     CDS             4545..5189
                     /locus_tag="PLX80_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19754.1"
                     /translation="MNFQSRILTVPKTARFSIKLPESGKADTFWLLIHGYAQLATDFL
                     SEFEFIKNEDHLLTAPEGLSKFYFRNKIGASWMTKEDRENEISDYVNYLNLLMEKIKK
                     EYDLKDATFNLLGFSQGVHTAVRFFIKGEYNFNNLILCSSDFPKDTDFIKLNEKLKKS
                     KMYFIHGTSDSAITNERFNESIKLLYENKIEFEDISFDGDHVLNKKLLFDLFCK"
     gene            complement(5246..6103)
                     /locus_tag="PLX80_02795"
     CDS             complement(5246..6103)
                     /locus_tag="PLX80_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQY19755.1"
                     /translation="MKLGKQYTSRENQSTDIYLKEISKTTPLSVEQEIDCAKRIKKND
                     KKALDLLVKANLRFVVSVAKQYQNQGLSLNDLINEGNLGLIKAAKKFDETRGFKFISY
                     AVWWIRQSILQALAEQSRVVRLPLNIVQQKSKIAKTISELEQKNERVPNVLEIAEKLD
                     MSVYEVQETLKNSGGHVSMDAPSIHGEDTKLIDIMPDKQEKMPDMTLLKDSLRIEIDR
                     ALDTLSQREKEVVKLYYGLEKENPLTLEEIGDKYKLTRERVRQIKEKAIRRLRQASRS
                     KALKAYLGQ"
     gene            complement(6208..6564)
                     /locus_tag="PLX80_02800"
     CDS             complement(6208..6564)
                     /locus_tag="PLX80_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19756.1"
                     /translation="MKSKYELFLDTDIILNESADGTLQENGLLSKCLKLFVCYTSVVN
                     ASEIFSGCTNKAELDNVKNSFTGINILGIPFRYSVKIGEILQLIKKKILAIITEMHWS
                     LQCAMKRNYRCLVQII"
     gene            complement(6765..7061)
                     /gene="rplW"
                     /locus_tag="PLX80_02805"
     CDS             complement(6765..7061)
                     /gene="rplW"
                     /locus_tag="PLX80_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQY19757.1"
                     /translation="MKIILVKPLITEKNTAIQEKLNQYSFEVGIKTNKLEIAKAVEDK
                     FNVRVKEVNTLISKGKKKSQMTKKGRFEGHRADRKKAIVTLHKEDKIDFLGNIE"
     gene            complement(7084..7713)
                     /gene="rplD"
                     /locus_tag="PLX80_02810"
     CDS             complement(7084..7713)
                     /gene="rplD"
                     /locus_tag="PLX80_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQY19758.1"
                     /translation="MEFKVFKLDGTESGESVNLPGEIFEIEPNHHLIYQAVRRYLSNQ
                     RQGTHKSKERSEVRGGGRKPFRQKGTGRARQGTIRSPLNVGGGTIFGPRPHTYKLKLP
                     KKAARLARRSALSIKAKENEIMIIQDFTFDSPKTKDIVNILKSLKIDETKTLLLTAAN
                     NENVYKSGRNIPKLNVMISDKASTYDLLNNKLILMQKSAVDSLCKSLLI"
     gene            complement(7716..8336)
                     /gene="rplC"
                     /locus_tag="PLX80_02815"
     CDS             complement(7716..8336)
                     /gene="rplC"
                     /locus_tag="PLX80_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQY19759.1"
                     /translation="MIGLLGKKIGMTTIFEGNGNMVPCTVIEAGPCYVTQIKSTETDG
                     YNAIQIGFEDKKEKNSTKPVIGKFKKINTPVQRYIKEIRDHSTENLKAGDVIKVDIFK
                     EGDTVKVSGVSIGKGFQGVVKRYGFAGGVRTHGQSDRERAPGSIGGSSYPSRVFKGQR
                     MAGRMGNDRITVRNLKVVKVYSDSNLILIKGAVPGAISGLVEIYKN"
     gene            complement(8360..8671)
                     /gene="rpsJ"
                     /locus_tag="PLX80_02820"
     CDS             complement(8360..8671)
                     /gene="rpsJ"
                     /locus_tag="PLX80_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQY19760.1"
                     /translation="MLASQNFRIKLKSYDHNLLDKWTERIIKTIKSTGAIVSGPIPLP
                     TRKNVYTVLRSPHVDKKSREQFETRSHKRLIDVLNSSNKTLEALTKLDPPGGVDIEIK
                     T"
     gene            complement(8690..9925)
                     /gene="tuf"
                     /locus_tag="PLX80_02825"
     CDS             complement(8690..9925)
                     /gene="tuf"
                     /locus_tag="PLX80_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HQY19761.1"
                     /translation="MAKEKYISDKPHVNIGTIGHVDHGKTTLTAAITMALAKKGHSQV
                     RAFDSIDKAPEERARGITIATAHVEYQTENRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGAMPQTKEHILLAKQVGVPAIVVFLNKEDIIRQQEKTEEDAELLLEVVES
                     ELRDILNGYDYPGDTIKIVRGSALKALEAGADASTTIDDERMKCIYDLMEACDSTIPE
                     PERDVDKNFLMPVEDVFSITGRGTVATGRIERGKVKVGEEVELIGLGAKKKTTVTGAE
                     MFNKELDEAVAGYNCGLLLRGVDKNEIERGMVLAKSGTITPHKKFEAQVYVLAKEEGG
                     RHTPFTTNYRPQFYFRTTDVTGVVHLPENVQMVMPGDNVDKLIIELISPIAMEDGLKF
                     AIREGGRTVGAGVVTKIIE"
     gene            complement(10060..10133)
                     /locus_tag="PLX80_02830"
     tRNA            complement(10060..10133)
                     /locus_tag="PLX80_02830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10097..10099),aa:Ile,seq:tat)
     gene            complement(10309..12402)
                     /gene="fusA"
                     /locus_tag="PLX80_02835"
     CDS             complement(10309..12402)
                     /gene="fusA"
                     /locus_tag="PLX80_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQY19762.1"
                     /translation="MSRQVSLDKTRNIGIMAHIDAGKTTTTERILYYTGRLHRIGEVH
                     EGAATMDWMEQEKERGITITSAATTCSWNDHRINIIDTPGHVDFTAEVERSLRVLDGA
                     VALFCSVGGVEPQSETVWRQADKYKVPRMAFVNKMDRTGADFFNAVEMMRERLKANAV
                     PIQLPIGQGVIFNGIIDLITMKARMYNEETLGATFEEVEIPDALKEQANEYRHKLLEA
                     VADVDDTLLEKFLDGKEISEDEIVKVLRKATIEVKIIPVLCGSSFKNKGVQNLLDRVV
                     DLLPSPLDVGNVEGHHLHTDDHIEREVKDDEKFTALAFKIMTDPFVGKLTFFRVYSGK
                     ALAGSYVYNSIAGKKERFSRLLQMHANSREDIKEVYCGDIAAAVGLKFTRTGDTLCDE
                     SDPIVLEKITFPEPVIQIAIEPKTKADQDKLGESLTKLSDEDPTFRVKTDEETGQTLI
                     AGMGELHLDIIVDRLRREFKVEANVGNPQVAYKETIRKKVTQEGKFVRQSGGRGQFGH
                     VWIEIEPNEKGKGFIFENAIVGGSIPKEYINPVAEGIEEAMKNGVLAGYPVEDIKVKL
                     YDGSYHDVDSSEMAFKIAGSMAFKEGAKKASPVILEPIMEVEVVTPEEYMGDVMGDLS
                     SRRGRIEGMSQRSDAQVIKSMVPLSEMFGYATTMRSMTQGRAIYTMQFSHYDEAPKSI
                     SDQIIEKIKGKEKVS"
     gene            complement(12456..12923)
                     /gene="rpsG"
                     /locus_tag="PLX80_02840"
     CDS             complement(12456..12923)
                     /gene="rpsG"
                     /locus_tag="PLX80_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQY19763.1"
                     /translation="MRRKRAEKRIRRPDTRYNDVLIGRFINCIMEDGKKQTAQRIMYK
                     ALDIIAEKTKDEPIEVFRKAVNNVQPAIEVRSRRVGGANYQVPSEVRPDRRIALAIKW
                     ILNYTESRNEKSMALKLANELMAAAVGEGNSVKKKEDVHKMAEANKAFAHFKW"
     gene            complement(12970..13353)
                     /gene="rpsL"
                     /locus_tag="PLX80_02845"
     CDS             complement(12970..13353)
                     /gene="rpsL"
                     /locus_tag="PLX80_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQY19764.1"
                     /translation="MPTINQLIRKGRKKIISKSKAPAMDSCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGAL
                     DTAGVANRKKSRSKYGAKRVKTASA"
     gene            complement(13525..15222)
                     /locus_tag="PLX80_02850"
     CDS             complement(13525..15222)
                     /locus_tag="PLX80_02850"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HQY19765.1"
                     /translation="MKRNSTFFIFFILILISFSIPGIVKSQIENVPLNNPVYIYLKEM
                     RVKRIINNFNDDNPNLSRFQVSDHLNEINLKKNELSKTEKELLEKYLIEFDPDYIDKK
                     TTMSLIESNMDVSNGFKYLFSDKQKYLFAYKKKENNIFIEALGNLYYINGIEPTVKKN
                     SWIFDVGLRVRGSLFDHLGYNFSILKGGASGDSVLIERAFPQIKANFKYQENIENITN
                     YDFTTGYLKYNIEPTEGMNISAQLGREKIVYGLGYSKRLALSGDAPDMDFLKFNFQYG
                     IINFSSIFGSTVGEYTPVRDDRYTKYFTANRLKLAFDNLFDIGLGETVISSRGIELGY
                     LNPIIFYKFVEHSLQDRDNGTIFVDLQTHFLKDLELQGTFFLDENILSNLSDMTKASN
                     KTAYQLGFFWYEPVGLENLSLIFEYTKIRPYVYSHFDIKNTYTAFGVNMGHPAGPNSD
                     QLFAKLTYNLNDNLRFKFEYQKIRKGENIYNQNGELVRNVGGDIFQPYIPEIDADEAY
                     FLDGERVNNFNLLFNVIYEPIKDFNFELNYTYNNDKYLSTGKSNDLSYGYLRFYFDY"
     gene            15349..16344
                     /locus_tag="PLX80_02855"
     CDS             15349..16344
                     /locus_tag="PLX80_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQY19766.1"
                     /translation="MYKPRYIELAESGELALRAETLRSMLTSCRICPKDCDVNRIENK
                     IAACYSGYLPVVSSYCIHYGEEPILVGSKESGNENGVGNIFFGNCNLRCVYCQNYEIS
                     QNHKTEIKNEISIQRLAEMMIELQEKKANAIGFVSPTHFVPQIVMALDIAVKNGLNIP
                     LVYNTNTYDSVEVLKLLEGIIDIYLPDLKYSDDENGYKYSKIKNYVLHSRNSIREMYR
                     QIGSRLIIEKGLLRRGLLIRHLILPNDLAGSKETLKFISDLDNNINVSIMSQYYPTNK
                     ALSIDLLSRNIKEKEYEKVIDLLDELDLENGFLQEFESENYYRPDFKDRVEPFKR"
     gene            complement(16360..16968)
                     /locus_tag="PLX80_02860"
     CDS             complement(16360..16968)
                     /locus_tag="PLX80_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQY19767.1"
                     /translation="MKDSYLTISEKSTAEIKISKSRFISNAHPISDREETTDIINDFK
                     KKYYDASHFPFAFRTGTDLNDYRYSDDGEPSGSAGKPLLESIDKSELTNVLVIVARYF
                     GGVKLGVGGLRRAFLAAGEECLKNAEIVKHLITKSFQIEFGYHFIGAIMNLLEKENIV
                     ILKNDSKEQCILKIEVRQSIVEDIILKFTEITNGDIKIHEIE"
     gene            complement(17039..17704)
                     /locus_tag="PLX80_02865"
     CDS             complement(17039..17704)
                     /locus_tag="PLX80_02865"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQY19768.1"
                     /translation="MDIYTLHRKELTEKLKSLGIKDLNVLKAVAKVKRENFVLEEFRK
                     YSYDNIALPLKSNQTISQPYTVAFMTELLEILPGDKVLEIGTGSGYQSAILAQMGAEV
                     YTIERIKELHERSSNKLKEYSYSVISKNDDGTKGWKEYSPFDKIIVTAGSPGYPKILL
                     SQLKIGGRMVIPVGDEKTQELMLIKKSVSLESNLPVYKAKKFKDFKFVPLIGEEGWKI
                     LEN"
     gene            complement(17742..18584)
                     /gene="cdaA"
                     /locus_tag="PLX80_02870"
     CDS             complement(17742..18584)
                     /gene="cdaA"
                     /locus_tag="PLX80_02870"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQY19769.1"
                     /translation="MELFKVGFFTVKLIDIVDILLVTYIFYKLYTVMKGTIASQIFFA
                     LVFVFGMSFIAQLLNMQTLGWLLGSITNIWIIALIVLFQPEIRRLLQLIGKTRVASIF
                     TNVNVDDNVSEIAEASFDLRNRGWGGLIVIERSSGLKSVMETGESVQSKINKEMIITI
                     FTPKSPLHDGAIILKNGKIEAARCLLPLSEHERIRNKDLGTRHRAGLGITEVSDAVAI
                     IISEERKVISVAENGKLEYYDNKDELLLRLSKAMGKESVANKVKDIFEESELEIEETD
                     DKKD"
     gene            complement(18589..19260)
                     /locus_tag="PLX80_02875"
     CDS             complement(18589..19260)
                     /locus_tag="PLX80_02875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY19770.1"
                     /translation="MGLIREYFLKNADQNNLNSISYKLRNNRKILFLEFISKIKRPVK
                     ILDLGGSEYFWRNLNLINLDDIQILLVNTEYQDVENISNINFINKDVRDLSEYSDKEF
                     DVVHSNSLIEHLSTFDEQKKLADEIQRIGRHYFIQTPNYYFPVEPHFLYPFFQFFSLK
                     KKTELIQKKDLGWYMKQENMDDAEALAASIRLMKKSELKNIFKNPKIHSEKYFLFTKS
                     FIVYN"
     gene            complement(19272..20960)
                     /locus_tag="PLX80_02880"
     CDS             complement(19272..20960)
                     /locus_tag="PLX80_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HQY19771.1"
                     /translation="MKKFLLIFILILIFKNTQSQVELVNVSNHVYHFLKRMQVMQIIP
                     DYNSSYAPLSRGVIASYLNIIKLNEKKLNSIDRNILKDLSVEFEYEMTGTLIKQNKLF
                     SKNGVDNIFNNDKEKYLYFATDSGNTFFSKLDINFSQRGSSGDSLLNNSLLLGQFGLE
                     FRGTLYDQVAYFLDLDFGAIFGNDSSDIAFASKTDNLLLGNYNFSQDMNNFNTFEGYL
                     RYQTESNWLSLMFGRSKFISGLGYIDKLFLSENSYPFDFGKLGINYKAVNYTFAYGSI
                     QGDSAEVYPVYTGVPLSAKNIATHSLNINFSNAFKLGLWETVIISQQPFSFTYLNPIS
                     FLTSADLGSGDEQTTKNNTLLGIEMEIIPVRNFSIQASLIVDDLTFGTLGKNDSLNEN
                     KYGWQFGTFWNTPYNADLIFEYTHLDPFVYSHRSNKSTYTNKGVPLGHSLPPNSDEIA
                     ARINYRFTNRIGVDLTYRFQRSGQGILLDSAGNLLANYGGDINFGLGDAYLRTNGFLD
                     GMRFNRSIITAEVYWEPVNQFIIEGKFENRVTSDLQNNSSVSDFFYFANVFMGF"
     gene            complement(21135..22658)
                     /locus_tag="PLX80_02885"
     CDS             complement(21135..22658)
                     /locus_tag="PLX80_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQY19772.1"
                     /translation="MIKIFSILLFLLTIILNSTDIYSQIDLKLNDNKKTTFEKLKNYD
                     DESKKLRSESNKLSLKYTNQPEKFNSENYFDTTKYHRFYPKYNKRESLMIPIYESAGM
                     NIGLALFNNYVGGASFAKITFKSVKHNLDTGFVWDDDVFVVNQFGHPFHGSIYFNTAR
                     STGFSYWESVPFAFGGSLMWEMFMETDPPQTNDIIQTTFGGVMVGEMTYRLTSLILDE
                     STRGANRTMREVVSAILNPARGFNRLVSGDMFRHTPTNVNDVFPVTSRLNVGYAGISA
                     SETVNFDKNHFMLEYLLVYGNNVGRDKEYKPFEFFRLRAGFDAAKADAPSTWIHAYGL
                     IYGKNILNKKNDDVLVFGVFHDYDYNYNNIIQLGTQSVGLGLIYDTPEKRKFRMVGTF
                     HSNFVVMGAINSIYQKGPFRNYDYTIGNKTMLEFGIYFKPVSLILDYKFYYLYTVNGF
                     PGHANFGILNPKLLVDIYKGFGIGTEYVYYHRHGTFEGVADYNKSISEFKIYGSYLF"
     gene            22755..23540
                     /locus_tag="PLX80_02890"
     CDS             22755..23540
                     /locus_tag="PLX80_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19773.1"
                     /translation="MTTFSNVYSQKKENIEDSPYGFKNISYLESISPDWNYKITLGAW
                     MNAMRGDISANQLYGYIDNQFYNKYNGADFSFYGYFQAKKNRGSLTAQFTSSNQSDKF
                     NYDNDSLYSRSITSSRSMFITTNMSYEFYRNSEVTFDLYGGARFNFITNLIENFYNTG
                     GSVQEGQTKFFIDPIIGTKFFYIPFKGPNISRLFINGNFDVGGYGLVSFLSFESYIGT
                     GYKINKSVTLNLGYRYIDVHYGTDTYLIDAGIQGIEFTATTNF"
     gene            23743..24531
                     /locus_tag="PLX80_02895"
     CDS             23743..24531
                     /locus_tag="PLX80_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQY19774.1"
                     /translation="MSDTNISVDPRFENTLEYRTKLSQFEGPLDILLHFVKVDDLDIY
                     NIPISKITKDFLEYINFMQDLDIELAGEFLVMASELMKIKARMLIPQIDEEGNVVEED
                     PRLTLVRKLIEYKRFKDVSEDISELESDQKKKSFRRNFENDIKEYETTEEIDRSLKNV
                     TIFNLIKAYKRVISSIRKEVIHPIELLDINPENQKEFLMNELDVKGEIEFDDLIKNIE
                     VKLQVICLFLAILQLALDGILEVKIIDNDMSRFMVCKRLNPELI"
     gene            24531..25772
                     /gene="scpB"
                     /locus_tag="PLX80_02900"
     CDS             24531..25772
                     /gene="scpB"
                     /locus_tag="PLX80_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQY19775.1"
                     /translation="MVSTEIKNIVEALIFASEEEITTRQIKEITDSSGVRISVKDIES
                     SIDSLNEDYRTFNKSFEIVQIAGGYSFSTKKEYGRFVGKLFEEKQKKKLSQSALESLS
                     IIAYRQPITRNEIEFVRGVNVDYIVNSLLERDLIKIHGRADTPGRPILYATTNTFLKV
                     LGLNSLEDLPKLKEINEILKNQEIEGITEADIELFNSANDPALTQPKETGQLELITSE
                     DIADETSENNDSIETENEIQITDENRAENSFDIIEDKEVINYQNSLSNLTKDEIEDEG
                     INIEYNDNEIQNDNESQNENDLEEIDNVEVANENAENEFNEVPVDKEIDADQNVDEEI
                     EGNDELLEINETDREREDNENDKDEINIKDGDSGNDIDLNFDNKDFENNEIKDHNENE
                     IHKDKPGDSDFLTKEGEEDSL"
     gene            25773..>26097
                     /locus_tag="PLX80_02905"
     CDS             25773..>26097
                     /locus_tag="PLX80_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19776.1"
                     /translation="MIRKKGPGNRKNSSGKDRFSKRGSGVKKEFPAKRKYSGKKEFSG
                     KDDFTDKKDFPDKSSPKEYYGKKESRGVRKFSDRSEKSFKKDTYGKKDNSGTKKYSDR
                     SDKKDY"
     assembly_gap    26098..26197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26198..27417
                     /locus_tag="PLX80_02910"
     CDS             <26198..27417
                     /locus_tag="PLX80_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY19777.1"
                     /translation="GKKEYSGTKKYSDRSDKKDYPGKKEYSGTKKYSDRSDKKDYPGK
                     KEYSGAKKYSDRSDKSFKKDTYGKNENSDPKKYAHSADKQGFYSRKENKNSSEYSDKK
                     DRYGKKEFKDTKDSSGKKYYKEKSDFYGKKDYRSRNELSDKQRYAKKKKIAPILKDPD
                     STEVYVKKEGEEGLIRLNKFIAANGIGSRRKVDEMIEQGRVTVNKKTITELGFKIDAE
                     NDKVKVDGELVKTDTKKVYIMMNKPKGVITSVSDEKKRTTVIDLINLNQKIYPVGRLD
                     YNTSGMLLLTNDGEFANHLMSPKNKVHKKYYVELSKPLEEKHRIKLCEGIKLDNVRTA
                     PAKMKFLKDNDYTRLYISIYEGRNRQIHNMFEHYGYFVRELIRVEYGGLKMEGLPEGS
                     WRYLTAEEIAKLK"
     gene            27510..29087
                     /gene="lysS"
                     /locus_tag="PLX80_02915"
     CDS             27510..29087
                     /gene="lysS"
                     /locus_tag="PLX80_02915"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQY19778.1"
                     /translation="MSEQNDLIKRRLEELEEIIAKGINPYPHKFDVTADSASLIKNYK
                     DVESDEEKEASKSEKVSVAGRIMAIRKMGKAAFFHIKDGTGRIQIYLKKDEIGDESYD
                     VFRLLDIGDIVGVNGYLFRTKTGEVSVHADSYVLLTKSIHPLPVVKEEVSETGEKIIH
                     DAFSDVELRYRQRYIDLIVNENVKDVFVKRTKIIQSIKRTLEKNDFFEVETPTLQSIY
                     GGANAQPFVTHHNSLDIDLYLRISNELFLKRLIVGGFNRVYEFVRDFRNEGIDRTHNP
                     EFTQVEWYQAYGDYFDSMKMFENVVEQACIDANGTTKVTYGEVELDFKSPWKRLRMTD
                     AIKEKTGLDVLEMKKQEIIDHMNRNGIEFDPKISHSKGLLIAHLFEETCEEDLIQPVF
                     IYDFPIDTTPLCKQLREYSMKQLDEMRNDPEQVIFAERFEPYVNKWEMGNSYTELNDP
                     ILQRKLLEEQVERGRAGEKETHPLDEDFLKAIEVGMPPTTGVGMGVDRLVMLLTNSQT
                     IRDVIFFPLMRPGKSNL"
BASE COUNT         9473 a         5135 c         4642 g        10011 t
ORIGIN      
        1 tttcaatact ttttgggtgt cattatctca cctataaaac atttaaaaac tttttaatat
       61 ctaaatttcc cgggaaccgg aaattaaaat atatttacgg ttatccgttt attatattta
      121 atttaccata tctctatgta atttataacc ttctcacctc aacaaagacg ccttactgga
      181 ttatcaatta cggttacatt cctttttata tatttcaggg agcagtatta ttcattggac
      241 tttatctaat tatcggaaag ctgataaaag ctccatttac aattccctat tattttttaa
      301 ataaaattaa atactttaaa gagagattta attctttctt cgccaaaaaa tctataaaga
      361 aattagattc ttcaagaaga gcttttataa aaacttctgc cgcatttgtg tcagggtacg
      421 cattcattgg cgctacgact ggtgtattaa gtaaaaatga ttatcagatt gaaagagtgg
      481 atctcagtat tccgggactt cctgaagaat taaaaggaac gacaatcaca tttataagtg
      541 atattcattc aggtccatat atgaatgaaa atctgatgag agaatatgct gaagtgataa
      601 atgacctcaa atctgatatg atcttaatgg caggtgatct tactaactca caaaaggaag
      661 aagtatttcc attcgtacat gctttcaaag ttctgaaagc tcagcatggt atttattctt
      721 ctcttggtaa tcacgattat ttttcagatg ctaattttat ttctaaggtg gtcaatagtg
      781 aatctccagt aaaaatactg agaaatcagt ctgaaatcat ttcaataaaa ggaaaagata
      841 tttgtataat gggaattgag gataccagac aaagcggatc agctccggat aacattctta
      901 ttaattatct tgatgaaacg atcatcggta ctaaaaaatt attcagtgat aaaaatctga
      961 attatgatga gactgctaaa attgctttgt tccataagcc atattttttc agtgaacttc
     1021 aggatagaaa aattgatttg ttcctgagcg gtcatactca cggcggacag gttgtacttg
     1081 cagattttgg aaatattaat atctcctttg caggtgcggt aagtgatttt atcaaaggtc
     1141 tctataaaaa tcctacttcg caaatgtatg tatcccgtgg aatcggctgt gtagctcttc
     1201 cgcttcgttt aaactgtcca cctgaaatta cagtctttcg cttagtttaa taagcaggta
     1261 tcccgttcgg atcaaatttt actaccttat ttgaaattgg tttttgagcc attagaaaat
     1321 caaaaatagc tcccagatct tcttctgaaa cttctgaata aaacgtccaa ggcattattg
     1381 tattaaattc tccctgatga atatttgcag cagtataatt tgaatcggaa aaactattaa
     1441 atcttttaat gaattgttct ttggtccagt ttcctatacc tgtttcttta tccggggtta
     1501 tattggaagt tctaataatt ccaccttttg gattcgggaa ttcgactcca cccgaaaaca
     1561 ttttatcctc aataaattca cctttctcca ttggagaatg acatgccata cagcctcctg
     1621 aataatattg tcccagtgct acaggatctg atgtttcagg tctattttta aaatcaggtt
     1681 tttgcggcaa tgttctgaaa atgagattca caggaaaatt caggctgtgt tcaggtattt
     1741 cattctttac agcaggcaat gtccttatat atgcaattat agcttttata tcttcttcat
     1801 ccatattcct gtaatacata aatggcatca ttggagcaag aggctctcca tccttatcta
     1861 cacccatagt gattgctctg aaaatttcac cgtctgtcca gtttcctagc ccagtctctt
     1921 tatcagaagt aatattcttt gccaatactg ttccggctcc ttcaccaaaa ttttcaccac
     1981 ccataccggc tgtcccttca atcatcggtc ctgcatattt actatagtct ctcttagagt
     2041 gacagtcgat gcatgaagct aacccatttg ctaaatattc tcctctttct atcatttcag
     2101 gtgtcgaaac aattttcatt tccggggcag gtcctacatc aggtaaactg gtttttatat
     2161 aaacaaaacc tgctatcaat aatacaacta ctaaccccag taaaatacca agaatcttga
     2221 aaagtttttt cattttttct tttaaattaa ttagtgaata tgtctgataa aaatttccac
     2281 taaaataaaa tttttaaatt taaatttcca tatttatttt tcatgaaaaa aattctgatc
     2341 gcctcaggta attctcacaa aatcaaagaa ataagttcta tagtctctga tattcccaat
     2401 cttgaagtga ctaccctcaa taattttgga atctgtctgg aggttgagga gaatggaaac
     2461 actttggaac agaatgcttt aataaaagcc aaatccgttt acagtctttt gggaatgccg
     2521 gttatctcag atgatacagg actttttgta aatgctctca atggcgaacc cgggatttat
     2581 tctgccagat atgcaggact gaatgctgct tataaagaca attgtgataa attattagat
     2641 aatctcaaag aaattgatga tgataattta caggcagagt ttaaatgctt tatttgtctt
     2701 ttgataaatc ctgaagagtt ttattttttt gaaggaattt gcagaggcag aatttgcaga
     2761 agtccgaaag gtgaaaatgg attcggatat gatcctgtat ttatgccaga tggttttgaa
     2821 aagacttttg ctgaattaac tgatgatgaa aaaaatttga tctcacacag aggaagagca
     2881 ttagaaaagt taaaaggatt tttaattaat ttccaataat atgatccctg tttagtattc
     2941 ctgcatagac atcaaagtta ccgttccttc catccgtcca tactgcaata atatctttgc
     3001 ctgaaactat aaaagaattg taatctccga aatactttcc gactgcaatc tctgaaggtg
     3061 aataaggatt tgtcgataaa tttttatccg taaaagtatt gcctccgtct gatgatactg
     3121 agatataaga ttcagttaat atgttattat tatcattcct ggaatcctga taaaaaatat
     3181 atatcaaccc attttcatct gtttcgatca atggttgata ctgatcattt tcagtttcat
     3241 ctgaattaat tctggcaggt attgtaaatt cagaattttc attaattagt tttgcaaaat
     3301 aaacatctga aagatcattg ccaatacctc ttgctgaata ggtaataaga atttctcctt
     3361 cagaagaaag ttcaagagac ggttcagaat tgatcctgat tcctgagctt tttactgatt
     3421 tcaaaatgct gttattttct acagatttcc ctgcagcatt tattttgagg taagatttta
     3481 tattgctcca ttcattccct tcatcaaaag atctaattac cttaatactt tttccgtcat
     3541 ttacatatgc aagatatata ttacctttgt tatcctctat catagaacta aaatgagact
     3601 tgtcatctgt taatgcaatc gggtccgaga acgactttcc tttatcttta ctcattatta
     3661 aatttatatg tgatttatag ccggaaattt ctgtccagta aatatagata atgtcatttt
     3721 tactgatgaa aatttttggc ttatcaatct taacagatga agttgaattg gaggcaagta
     3781 aatttttatc agaagtccag tttattccat tatcatttga cctggcaaaa aatatacctt
     3841 catttatatt tttatgcata tcatactgaa tcgatgtaaa aaataattca ctgttattat
     3901 catattctat ccaggggtcc gaataagaag aagcactata acattcggaa agatctatct
     3961 cccctgcttt ccagtctaat cccttattta cagaaataaa tattcttgct ttattttcat
     4021 caatcgagaa atcatttgca gaaacaatta aaatttgatt actcgtagga ctgcacttga
     4081 tcacaggttc agaagctccg gggagttcat ttattctgat attggtaagt tcttttttgc
     4141 gggaaatatc tttagaggta tatttatctg cttttccaaa tttatcatta tacataaatt
     4201 tctttggtga catctgaatc tcaggttcac taatatcttt cttgcttaaa ttcagataaa
     4261 taaaacctga tacaaagata agcaaagaaa aaagagcaga aaatattttt ttaaaattct
     4321 tattcataaa tatcgtaccc cgtagaagat tcgaacttct aaatacagat tcgtagtctg
     4381 ttggtttatc cattagccta acggggcgtg atgcaaaatt aattttttca atatgcaata
     4441 aaaaggaaat tcttaacagt atttaaatcc tcaattgtat gaaaattaaa gttacaattt
     4501 attcctcact tagatatatt tacgactaaa aaatacaata cttaatgaat tttcaatcaa
     4561 gaatattaac tgttccaaaa actgccagat tttcaattaa gcttcctgag tcaggaaaag
     4621 cagatacatt ctggcttctc atccatggat atgctcagct tgcaacagat tttttatctg
     4681 agtttgaatt tataaaaaat gaagatcatt tactgacagc tcctgaaggt ctgtcaaaat
     4741 tttatttcag aaataagatc ggtgcttctt ggatgactaa agaagacaga gaaaatgaaa
     4801 tctctgacta tgttaattat ctgaatttac ttatggaaaa aataaaaaaa gagtatgatc
     4861 tgaaagatgc aacatttaat cttcttggat tttctcaagg tgtgcatact gcagtcagat
     4921 tttttattaa aggtgaatat aatttcaata atctcatatt atgttcatct gattttccga
     4981 aagatactga ttttataaag ttgaatgaaa agttaaagaa gtctaagatg tatttcattc
     5041 atggcacttc tgattcagca attaccaatg aaagatttaa cgaaagcata aaattacttt
     5101 atgaaaataa aattgaattt gaagacatat cttttgatgg agatcatgtc ctgaataaaa
     5161 aattactttt cgatcttttt tgcaaataaa aaaagtatcc gctaaaaaat taacggatac
     5221 tttcattaac agataaaatt attttttact gacctaagta agcttttaat gctttacttc
     5281 tggaagcctg tcttaatctt ctgatcgctt tttccttgat ctgtcttact ctttcgcggg
     5341 tgagtttgta tttgtcacct atttcttcaa gtgtcagcgg attttctttt tcgagaccat
     5401 aataaagttt tacaacttct ttttctctct gagataaagt atcaagtgct ctgtcaattt
     5461 ctattctgag tgaatctttc agtaatgtca tatcaggcat tttctcctgt ttgtcaggca
     5521 taatgtcgat aagctttgta tcttcaccgt gaatactcgg agcatccata gatacgtgac
     5581 cgcctgaatt tttaagggtc tcctgtactt cataaacact catatctaat ttctcagcta
     5641 tctcaagtac gttaggaact ctttcatttt tctgctcaag ttcactgata gtttttgcga
     5701 tcttactttt ttgctgaacg atattcagcg gtaatctcac aactcttgac tgctcagcta
     5761 atgcctgaag aatggactgt ctgatccacc atactgcata agaaataaat ttaaatcctc
     5821 ttgtttcgtc aaattttttg gcagctttta taagtccgag gtttccttca ttgatcagat
     5881 cattcagaga caaaccctga ttctgatact gcttggcaac actaactaca aatcttaaat
     5941 ttgctttgac caaaagatcg agtgcctttt tatcattttt ctttattctt ttcgcgcaat
     6001 ctatttcctg ttcaactgaa agcggagtag ttttactgat ttcctttaaa taaatatcag
     6061 tagactgatt ttccctgctt gtatactgtt tgcctaattt cataaattat ttatttaatt
     6121 ttaatatttt atctggtgaa ttatatttca aaatttcttc ctgggatatg agcttgatat
     6181 tcagcaactt tgctatcttc cgatatttta aattatctgc actaagcagc ggtaatttcg
     6241 tttcatagca cattgcaagg accaatgcat ctctgtaatt attgccaaga tttttttttt
     6301 aatcaattga agaatttctc ctatttttac agaatatctg aatggaattc cgagaatatt
     6361 tattccggtg aatgagtttt ttacgttatc aagttcggct ttgttagtgc atcctgaaaa
     6421 aatttctgat gcatttacta cagaagtata acatacaaat aacttcagac attttgacaa
     6481 caagccattt tcctgaagtg ttccatcggc agattcattt aaaataatat ctgtatcaag
     6541 aaataattcg tatttacttt tcatataaaa aatgctgagt taacatttac acaaatccag
     6601 ttacaatgtt ataagaaaat aaaagctcaa taactgaatt atcgagctta tataaaattg
     6661 tgttacttca attgaaattc taaaataaga gatgtcttta gattaaagat ttgacaatat
     6721 aggtaaggaa aagaacgaaa actactagac ttgctctgat tcttttactc tatattacca
     6781 agaaaatcta tcttgtcttc tttatgcaaa gtaacaatag ctttttttct gtcagctctg
     6841 tgaccttcaa atctaccctt tttagtcatt tgtgatttct tcttaccttt agagataagg
     6901 gtatttactt ccttcactct gacattaaat ttatcttcaa ctgcttttgc aatttcaagc
     6961 ttgttggtct taatacctac ttcaaaagaa tactgattca gtttttcctg aatggcagta
     7021 tttttttcgg taataagcgg ttttactaat attattttca tatatataaa atttcagatg
     7081 atattaaatt aataaactct tacataatga atcaacagca gatttttgca tgagtatcag
     7141 tttattattc aaaagatcat aagtcgaagc tttatcagag atcattacat ttagctttgg
     7201 gatatttctt cctgatttat aaacattctc attatttgct gcagtcagca aaagagtttt
     7261 agtttcatca atctttagtg acttaagaat attgactata tcttttgtct taggggaatc
     7321 aaaagtaaaa tcctgaatta tcatgatctc attctctttt gcctttatac tgagagcact
     7381 tcttcttgca agtctggcag ctttcttggg aagtttcaat ttataagtat gaggtcttgg
     7441 tccaaagatt gtaccaccgc caacatttag aggagatctg attgtacctt gtcttgcacg
     7501 tccggtacct ttttgacgaa atggcttcct tcctccgcct ctgacttcac ttctctcttt
     7561 agacttatga gttccctgtc tttgattgga taaatatctt ctcactgcct gatagatcag
     7621 gtgatgattt ggttcaattt caaatatttc tccgggtaaa tttactgact ctccggattc
     7681 agtaccatcg agtttaaata ctttaaattc catttttaat ttttataaat ttcaactaat
     7741 ccggatattg ccccgggtac tgcgccttta atcagtatca agtttgaatc actatatact
     7801 tttacaactt ttaaattacg gactgtgatc ctgtcatttc ccattcttcc cgccattctc
     7861 tgacctttaa aaactctgga aggataagaa ctaccaccga tagatccggg agctctttct
     7921 ctgtcgctct gtccgtgagt tcttacacca cctgcaaaac cgtatctttt tacgacacct
     7981 tgaaatcctt ttccgataga aacaccggaa acttttactg tgtcgccttc tttaaaaata
     8041 tcaaccttaa ttacatcacc ggcttttaaa ttttctgtag aatgatctcg aatttcttta
     8101 atatatcttt gtactggtgt attgatcttt ttgaatttac ctatgactgg ttttgtagaa
     8161 tttttttctt tcttatcttc aaaaccgatc tgtatggcat tatatccatc tgtttcagtg
     8221 cttttaatct gtgtaacata acacggacct gcctctatta cagtgcatgg caccatattt
     8281 ccgttacctt cgaagatagt agtcataccg atcttcttac caagtaaacc gatcataaaa
     8341 atttatttat ctttttaaat tatgttttaa tttcaatatc gactccaccg ggcggatcaa
     8401 gtttcgtcag agcttcaaga gttttgtttg atgagttcaa tacatcgatc aatctcttat
     8461 gtgatcttgt ctcaaactgc tctcttgatt ttttatcaac gtgaggtgat cgcagcaccg
     8521 tatatacatt tttccttgtg ggaagcggta taggtccgct gacaattgca cctgttgact
     8581 ttattgtctt gattattctt tccgtccact tgtcaagcaa gttgtgatcg taagatttta
     8641 acttgattct gaaattttgc gaagccaata tattatttta gtttaattat tattcaataa
     8701 tttttgtaac aacacctgca cctacagttc ttccgccttc tctgatagca aactttaatc
     8761 cgtcttccat cgcgataggt gaaataagtt caatgatcag cttatcaacg ttgtctccgg
     8821 gcattaccat ctgaacattt tcaggaaggt gaacgacacc agttacgtct gtagttctga
     8881 aataaaactg aggtctgtaa tttgttgtga atggagtgtg tcttccaccc tcttcttttg
     8941 caagtacata aacctgagct tcgaattttt tatgtggtgt aatagtaccg gattttgcaa
     9001 gtaccattcc tctttcgatc tcatttttat caacacctct taaaagcaat ccgcagttat
     9061 atcctgctac tgcttcatca agttctttat tgaacatttc tgcacctgtt acagtagttt
     9121 tcttttttgc tccaagtccg ataagttcaa cttcttcacc aacttttact ttacctcttt
     9181 caattcttcc tgttgctaca gttcctctac ctgtaataga gaatacatcc tctaccggca
     9241 taaggaaatt tttgtcaaca tctctttccg gctcaggaat tgtagaatca caagcttcca
     9301 taagatcata aatacatttc attctttcat catcaatagt tgtactggca tctgctcctg
     9361 cttctaaagc tttcagagca cttcctctta caatcttaat tgtatcaccc ggataatcat
     9421 atccgtttaa tatatctctt aattcagatt ccacaacttc aagaagtaat tcagcatctt
     9481 cttcagtttt ttcctgctgt ctgattatat cttctttatt taagaaaaca actatagctg
     9541 gaacacctac ctgctttgca agtaaaatat gttcttttgt ctgaggcata gcaccgtcag
     9601 ttgctgcaac tactaagatt gctccgtcca tctgagcagc acctgtgatc atatttttaa
     9661 tatagtcagc gtgtcccgga cagtcaacgt gtgcatagtg tctgttttca gtctgatatt
     9721 ctacgtgagc agttgcaatc gtaattcctc ttgctctctc ctcaggagct ttgtcaatag
     9781 agtcaaaagc tcttacctga gaatgacctt tctttgccaa agccattgtt attgctgctg
     9841 tcagggtagt tttaccgtga tcaacgtgtc caatagttcc gatattaaca tgtggtttat
     9901 cggaaatgta tttttctttt gccattacta tttctcctta agagtttatt ttttaattta
     9961 attaatttga atttctgaat ctgaatcaaa gtttcattaa aattcctttt taatttcctc
    10021 ttattgtaca ttgtacatta aaaattgaaa attaaaaagt gggtcaggga ggaattgaac
    10081 ctcccacctc gtgcttataa ggcacgcgct ctaaccatct gagctactga ccctggatgg
    10141 aaactcattc ctattgtata aagaatcttt ataataaaaa tggattttat agaacgactt
    10201 aatgtgtctg atatacgagt tatactccgg cgaaccggaa ctgacccaga taacttattt
    10261 taaagaacaa taaaactttc tgttgcaatt taaatctttt ataactattt aagaaacttt
    10321 ctctttacct tttattttct caatgatctg atccgaaata ctctttggag cttcatcata
    10381 atgagagaac tgcattgtgt aaattgctct accctgtgtc atcgatctca ttgtagttgc
    10441 atatccaaac atttcagaaa gcggtaccat tgacttaata acctgtgcat cacttctctg
    10501 tgacatacct tctattctgc ctcttcttga gctgagatca cccatgacat ctcccatata
    10561 ttcttcagga gttacaactt ccacttccat gatcggctca agtattaccg ggcttgcttt
    10621 tttagctccc tctttaaatg ccattgaacc tgcgatctta aaagccattt cagatgagtc
    10681 aacatcatga tatgaaccgt catacaattt tacctttata tcttcaacag gatatcctgc
    10741 aaggactccg tttttcattg cttcttcaat accttctgca accggattga tatattcctt
    10801 tggaatagat ccaccaacaa ttgcattttc aaaaataaat ccttttcctt tttcattcgg
    10861 ctcgatctcg atccacacat gtccaaattg tcctcttcca cctgattgtc tgacgaattt
    10921 tccttcctga gtaactttct ttctgatagt ttctttataa gctacctgcg gatttcccac
    10981 attagcttct actttaaatt ctcttcttaa tctgtctaca ataatatcca gatgaagctc
    11041 acccatacct gcgattaaag tctgtcctgt ttcttcatct gtcttcactc tgaatgttgg
    11101 atcttcatca gaaagctttg taagtgattc accaagttta tcctgatcag ctttggtttt
    11161 tggctcaatt gcaatttgaa taaccggctc tgggaatgta attttctcaa gtacgatcgg
    11221 atcactttca tcacaaagcg tatcacctgt tcttgtaaat ttcaaaccaa ctgcagctgc
    11281 tatatctccg cagtaaactt ctttaatatc ctctctgctg ttagcatgca tttgaagaag
    11341 tctgctgaat ctctctttct ttcctgcaat tgaattataa acataacttc ccgctaatgc
    11401 ttttcctgaa tatactctaa agaatgtaag ttttccaaca aatggatcag tcattatctt
    11461 aaatgccaat gctgtaaatt tttcatcatc tttaacttct ctttctatat gatcatctgt
    11521 atgaagatga tgaccttcta catttcctac atcaagcggt gaaggaagaa gatcaacaac
    11581 tctgtccaat aaattctgaa cacctttatt tttaaatgat gatccgcata gtactgggat
    11641 tatcttaact tcaatagttg cttttctgag cactttaact atttcgtctt cacttatctc
    11701 tttaccatct aaaaactttt ccagtaaagt atcatcgaca tcagctactg cttcaagcaa
    11761 tttatgtctg tattcatttg cctgctcttt taatgcatct gggatttcaa cttcttcaaa
    11821 tgttgcacca agtgtctctt cattatacat tcttgctttc atcgtaataa gatcaatgat
    11881 tccgttaaat ataactccct gaccgatagg caattgaatt ggaactgcat tggctttcag
    11941 tctttctctc atcatctcaa ctgcattaaa aaaatctgct ccggtcctgt ccattttatt
    12001 tacaaatgcc attctcggca ctttgtattt atcggcttgt ctccagactg tttctgactg
    12061 cggctctaca ccaccgacag aacaaaataa cgcaactgca ccgtctaata ctcttaaaga
    12121 tctttctacc tcagcagtaa aatcaacgtg tccgggtgta tctataatat tgatcctgtg
    12181 atcattccat gagcaagtag ttgctgcaga agtaattgta atacctcttt ctttttcctg
    12241 ctccatccag tccattgttg ctgcgccttc atgtacttca ccaatcctgt gcaatctgcc
    12301 tgtataatat agaatacgct ctgttgttgt cgtttttccg gcgtcaatat gcgccattat
    12361 tcctatattt ctcgttttat ctaagcttac ttgtcttgac atattatttg atttctatct
    12421 ttttattaat gttaattgtg tttataaaaa attgtttacc acttaaaatg agcaaatgct
    12481 ttatttgctt cagccatttt atgaacatcc tctttctttt tcactgaatt accttcgccc
    12541 actgctgcag ccattaattc gttagcaagt ttcaaagcca tggatttttc atttcttgat
    12601 tcagtgtaat taagaatcca ttttattgca agagcaattc ttctgtcagg tcttacttct
    12661 gatggtacct gataatttgc acctccgact cttcttgatc tgacttcaat cgcaggctga
    12721 acattgttga cagcctttct aaaaacttcg atcggttcat cttttgtttt ctcagcaata
    12781 atatccaatg ctttatacat tatcctttgt gctgtctgct ttttaccgtc ttccattata
    12841 caattaatga atcttccgat taggacatca ttatatctgg tgtcaggtct tctgatccgc
    12901 ttttcagctc gttttcttct catttatata tgaaataaaa atttaaattc ttttaataat
    12961 taaataattt tatgccgaag ctgttttaac tcttttggct ccgtactttg accgtgattt
    13021 tttacggtta gcaacaccgg ctgtatccaa tgctccacgt atgatatgat atcttacgcc
    13081 cggaagatct ttcacccttc cgcctctgat cagtacgatc gagtgctctt gtaaattatg
    13141 tccttctccc ggtatataag ctgtaacttc aataccgttt gttaatctta ctctcgctac
    13201 ttttctgaga gcagaatttg gtttctttgg cgtagaagta tatactcttg tacaaacacc
    13261 tcttttctgt ggacatgagt ccatagccgg tgctttactc ttagaaatta tcttctttct
    13321 acctttgcgt attagctgat ttattgtagg caatgtaatt attacttatt ttggaaacct
    13381 ataaaataca cattatattg ctttgagtca atttatttta cagtgcataa cctaactttt
    13441 tttgggatgt gaaaatgaat tataagaaaa attacagatt tgttttgaat gatcataaca
    13501 aaaaatattc gactcgctgt gaattcaata atcaaagtag aatctgagat atccataact
    13561 tagatcatta ctttttcctg tggataaata tttgtcatta ttataggtat aattcaattc
    13621 gaaattaaaa tcctttatcg gttcataaat cacattaaaa agtaaattaa aattattcac
    13681 tctttctccg tctaaaaaat atgcttcgtc tgcatcaatc tcaggaatat atggctggaa
    13741 aatatctcct ccgacatttc gaacaagctc cccgttttga ttataaatat tttcaccttt
    13801 tctgattttc tgatattcaa atttaaatct taaattatca ttaagattgt aagtcagttt
    13861 tgcaaacaac tggtccgagt ttggaccggc aggatgaccc atgtttactc caaatgctgt
    13921 gtacgtattt tttatatcaa agtgagaata aacgtaaggt cttatttttg tatattcaaa
    13981 tatcagcgat aaattttcaa gaccgacagg ctcataccaa aaaaatccca gctgatatgc
    14041 agttttgttt gaagctttag tcatgtctga taaattagaa agtatgtttt catcaagaaa
    14101 aaaagtaccc tgcagttcaa ggtcttttag aaaatgagtc tgaagatcaa cgaagatagt
    14161 tccgttatct ctgtcctgaa gtgaatgttc aacaaactta taaaatataa ttggattcag
    14221 atatccaagt tcaatgcctc tcgaagaaat tacagtttct cccaaaccaa tatcaaaaag
    14281 attgtcaaag gctaatttca gtctgtttgc tgtaaaatat tttgtatacc tgtcatctct
    14341 taccggtgta tattcaccaa ctgtcgatcc gaaaattgat gaaaaattta ttataccgta
    14401 ttgaaaatta aatttcaaaa aatccatgtc aggtgcatct cctgataatg caagtctctt
    14461 actataccct aatccgtaaa ctatcttttc tctaccaagc tgcgctgaaa tattcatccc
    14521 ttctgtcggc tctatattat attttaaata accagtggta aaatcataat tcgtaatatt
    14581 ctctatattt tcctgatatt taaaatttgc ttttatctga gggaatgcac gttctatcaa
    14641 taccgaatct cctgaagctc cgccctttaa tattgaaaag ttgtaaccca gatgatcaaa
    14701 caaagaacct ctgactctta atccaacatc aaatatccat gaattttttt ttactgtcgg
    14761 ctctattcca tttatataat acaaatttcc aagagcttct ataaatatat tattttcttt
    14821 tttcttatat gcaaacaggt atttctgttt gtcggaaaat aaatatttga aaccatttga
    14881 aacatccatg ttgctctcga tcaaagacat tgttgttttc ttatcaatgt aatccggatc
    14941 aaattcaatc agatattttt cgagaagttc cttctctgtt ttactcagtt catttttttt
    15001 tagatttatc tcattcagat gatcacttac ctgaaatctt gacagatttg gattgtcatc
    15061 attaaaatta tttatgatcc tttttactct catttctttt agataaatat aaaccggatt
    15121 attcagcggt acattttcga tctgagattt tacaattcct ggaatggaaa aagaaattaa
    15181 tatgagaata aaaaatataa agaaagtaga atttcgtttc attaatttaa aaagttttcc
    15241 caatataata attagtaagt acataattaa tttaaaaaca atggtaaggt ttttgtgaat
    15301 ttaatatcag ctttaatttt gataaaatat tttatttcgt aatttagtat gtataaaccc
    15361 agatatatcg aacttgctga atcaggcgaa ctcgctttga gagctgaaac tttaagatca
    15421 atgttaactt catgcagaat ttgtcccaaa gactgcgatg taaacagaat tgaaaataag
    15481 atagctgcat gctattcagg ctatctgcct gttgtatctt cttactgtat tcattatggg
    15541 gaagaaccaa ttcttgtagg ctcgaaggaa tccggtaatg aaaatggtgt cggtaatatt
    15601 ttttttggca actgtaatct gagatgtgtc tattgtcaaa attatgagat ctctcaaaat
    15661 cataagactg aaataaaaaa tgaaatttca attcaacggc ttgccgaaat gatgatagag
    15721 cttcaggaaa aaaaagctaa cgccattgga tttgtttctc ccactcattt cgtaccgcag
    15781 attgttatgg ctctggacat tgctgtaaaa aatggattga atattccgct ggtttacaat
    15841 acaaacactt acgactcagt agaggtcttg aaattactcg aaggcataat tgatatttat
    15901 ttacctgatc tgaaatattc tgatgatgaa aacggatata aatattccaa aataaaaaat
    15961 tatgttttgc attcaagaaa ttccatcaga gaaatgtaca gacagatcgg aagccgttta
    16021 attattgaaa agggattgct cagaagaggt ttgctcatca gacatttaat tttgcctaat
    16081 gatctcgccg gcagtaagga aactctgaaa tttatttccg atcttgataa taacattaat
    16141 gtttctatta tgtctcaata ttatcctact aataaggcat tgtcaataga tcttctttca
    16201 agaaacatta aagaaaaaga atatgaaaaa gtaattgatc ttttagacga gctggatctt
    16261 gagaatggat ttcttcagga gtttgaatct gagaattatt accgtcccga ttttaaagac
    16321 agagtagaac catttaaaag ataaatatca tttcatacat tattcaattt catgaatttt
    16381 aatatctcca tttgtaattt ccgtaaactt taaaattata tcttcgacta tagattgcct
    16441 cacctcaatt tttaaaatgc attgttcttt agaatcattt ttaagaatga ctatattttc
    16501 tttttctaaa agattcatga ttgctccaat aaaatgatat ccgaattcaa tctgaaaaga
    16561 cttagttatt aaatgtttta caatctccgc atttttaaga cactcttccc ctgctgctaa
    16621 aaatgccctg cgcaaacctc ccactccaag ctttactccg ccgaaatatc ttgcgactat
    16681 gaccaataca tttgtaagtt cagacttatc tattgattcg agtaaaggtt ttcctgcgct
    16741 gccggaaggt tctccatcat ccgaataccg ataatcattc aagtcagtac ctgtcctgaa
    16801 cgcaaatggg aaatgcgatg catcataata tttttttttg aaatcattta ttatatcggt
    16861 agtttcttct ctgtctgaga ttgggtgagc attggaaatg aatctggact tgctgatctt
    16921 aatttcagca gtactttttt cggagatcgt aagataagaa tctttcaaat taaaactttg
    16981 attttcttag attcaaatat aattgaatct aattatgtaa actgaaactt cagctaattt
    17041 aattttcaag aattttccat ccctcttcac caatgagcgg aacaaattta aaatctttaa
    17101 atttctttgc tttatatacc ggtaagtttg attctaaact gacagatttt ttaataagca
    17161 taagctcctg agttttctca tccccaactg gtattaccat tctcccaccg attttcaact
    17221 ggctcaataa aatttttgga tacccgggac ttccggctgt tactataatt ttatcaaaag
    17281 gtgaatattc tttccaaccc tttgtgccgt catcattttt tgaaattaca gaataactat
    17341 attcctttaa tttatttgat gacctttcat gaagttcttt tattctttct atagtatata
    17401 cttcagctcc catctgagca agtatggctg actgatatcc ggatccggtt cctatctcaa
    17461 ggactttatc tcccggaagt atttcaagaa gctcggtcat aaatgcaact gtatatggct
    17521 gtgaaatggt ctgatttgat ttcaatggta aagcaatatt atcgtatgaa tattttctga
    17581 attcttcgag tacaaaattt tctcttttaa ctttcgcaac agctttcaat acattcaaat
    17641 ctttaatccc gagtgacttt agtttttcag taagttcttt cctgtgcaga gtatagatat
    17701 ccaattcaat aataaattat aatttcaaat aattctaaat tctaatcctt tttatcatca
    17761 gtttcttcga tctcaagttc agattcttca aatatatctt taactttatt tgctacagat
    17821 tctttaccca ttgctttgct tagtcttaaa aggagttcgt ctttattatc ataatactca
    17881 agctttccgt tttcagctac agagataact tttctttctt cagaaattat tattgccact
    17941 gcatctgaaa cttccgttat tcccagtcct gctctgtgtc tcgtgccaag atctttattt
    18001 cttattcgtt catgttctga taaaggaagc agacatcttg ccgcttcaat ttttccgttc
    18061 tttaatatta tagcaccatc atgcagcgga gatttcggag tgaatattgt aatgatcatt
    18121 tctttattaa tttttgactg aactgattct cctgtttcca ttacgctttt taatcctgag
    18181 cttctttcaa ttacaatcaa tcctccccac cctctgttcc tcaaatcaaa acttgcttct
    18241 gcaatttcac tcacattgtc atcgacattc acatttgtaa aaatacttgc gactctggtc
    18301 ttcccgataa gctgaagcag tcttctgatc tctggctgaa ataaaactat aagagcaatt
    18361 atccagatat ttgtaatgct gccaagaagc catcccagtg tctgcatatt caaaagctgc
    18421 gctatgaatg acattccaaa tacaaaaacc aaagcaaaga agatctgcga agcgatcgta
    18481 cccttcatta ctgtataaag cttatagaaa atataagtga caagaagtat gtcaactata
    18541 tcaataagct tgaccgtaaa aaatcctact ttgaataatt ccatttacct aattataaac
    18601 aataaaagat tttgtaaaaa ggaaatactt ttcactgtga atttttggat ttttaaaaat
    18661 attctttagc tcactctttt tcattaatct gattgaagca gctaatgctt cagcatcatc
    18721 catattttcc tgcttcatgt accagcccag atcctttttt tgtatcaatt cagttttttt
    18781 cttaagtgaa aaaaattgaa aaaaaggata aagaaaatgc ggctctacag ggaaataata
    18841 atttggtgtc tgtataaaat aatgtctgcc gatcctctga atctcatccg ccagtttttt
    18901 ctgttcgtcg aaagtactca aatgctctat cagactgtta gaatgtacga catcaaattc
    18961 cttatcagaa tattccgaca gatctctcac atctttattt ataaaattaa tattactaat
    19021 attttcaaca tcttgatatt cagtatttac aagcagtatc tgtatatcat caagattgat
    19081 cagattaaga ttcctccaaa aatattcact gcctccgaga tcaagtattt tcaccggtcg
    19141 ctttatttta ctgatgaatt ctaagaacag tatctttcgg ttatttctta atttgtaaga
    19201 tatagaattt aaattattct gatcagcatt ctttaaaaaa tattctctga ttaatcccat
    19261 ttttaaaaaa attaaaaacc catgaataca tttgcaaaat aaaagaaatc agaaacactt
    19321 gaattattct gaagatcact cgtaactctg ttttcaaatt taccttcaat tataaactga
    19381 ttaaccggct cccagtaaac ttctgccgta ataatacttc tgttaaatct cattccatca
    19441 agaaaaccat ttgtccgaag atatgcatca cctagtccaa agtttatgtc gccgccgtaa
    19501 tttgcaagaa gattacctgc gctgtcaagc agaatcccct gccctgacct ctgaaatctg
    19561 taagtaagat caacgccaat tctgtttgta aatctgtaat taattctcgc tgcaatctca
    19621 tctgaatttg gcggaagtga atgtccaagc ggtacacctt tatttgtata tgtacttttg
    19681 tttgacctgt gtgaataaac aaacggatcc aaatgagtat attcaaatat cagatccgca
    19741 ttataaggag tattccagaa tgtaccaaac tgccagccgt atttattttc attcagtgaa
    19801 tcatttttac caagcgtacc aaacgtcaga tcatctacga tcagagaagc ctgtatagaa
    19861 aaatttctga cagggataat ttccatttca ataccgagca atgtattatt tttagttgtc
    19921 tgctcatcgc ctgatcccag gtctgctgat gttagaaaac ttataggatt tagataagtg
    19981 aaactaaaag gctgctgact gatgatgaca gtttcccaaa gtccgagttt aaacgcattt
    20041 gaaaaattaa tattcaaaga atgagttgca atattttttg cggataaagg aacacctgtg
    20101 tacacaggat atacttcagc cgaatctccc tgaatgcttc catatgcaaa agtataatta
    20161 acggctttgt aatttatgcc gagctttcca aaatcaaacg gataagaatt ttcagataaa
    20221 aataatttat caatatagcc aagacctgag ataaattttg atctgccgaa catcagcgaa
    20281 agccagttac tttctgtctg atatctcaga tagccttcga atgtattaaa attattcatg
    20341 tcctgtgaaa aattataatt tcccaaaagt aaattatctg tctttgatgc aaatgcaata
    20401 tcactgctgt catttccaaa tattgctcca aagtcaagat ccagaaaata tgcgacctga
    20461 tcataaagtg tgcctcggaa ttccagcccg aactgtccga gcagtaaaga attatttaat
    20521 aaagaatcac ctgaagatcc tcgctgagaa aagtttatgt caagctttga aaaaaaagta
    20581 ttcccagaat cagttgcaaa gtaaagatat ttttctttat cattattaaa aatattatca
    20641 acaccgtttt tactaaacag tttgttttgc ttaatcagag ttccggtcat ttcgtattca
    20701 aactcaactg aaagatcttt cagaatattt ctgtcaatgc tgttgagttt tttttcattc
    20761 agttttataa tatttaaata tgaggcaata actccccggg acaaaggtgc ataagatgaa
    20821 ttataatcag ggatgatctg catgacctgc atacgtttta gaaaatggta aacatgattt
    20881 gatacattga caagctcgac ctgagactgt gtgtttttga agatcagaat taatatgaaa
    20941 ataagtagaa attttttcaa acgccggatt aatgtttttc aaattaatta attaaaatca
    21001 gtatttaaat atagaagaga ggacgaaaca caattacaaa tttcaaatta caaattgcaa
    21061 ttacccaata gattataagc taaattaaaa atgacatgat gatttactta atcattttca
    21121 atttcatgaa caaatcagaa taaataagat ccgtaaattt tgaattcact tatactctta
    21181 ttataatctg ctactccttc aaaagtacca tgtctgtgat agtagacata ttctgtgcca
    21241 attccgaatc ctttatatat atcaacaaga agtttaggat ttaatattcc aaagtttgca
    21301 tgtccgggga aaccgtttac agtatataaa taataaaatt tataatcgag tatcagactt
    21361 acaggtttaa aataaattcc gaattcaagc atggttttat ttccgatcgt ataatcgtaa
    21421 tttcggaatg gacctttctg ataaatagaa ttgatagcac ccattacaac aaaatttgaa
    21481 tggaaagtac ctaccattcg aaattttctt ttttcaggag tatcgtaaat tagtcccaga
    21541 ccgacactct gagtacccag ctgaattata ttattataat tataatcata atcatggaaa
    21601 actccgaaga caaggacatc atcattcttt ttatttaaaa tattcttccc gtatatcaga
    21661 ccataagcat ggatccatgt agatggtgca tctgcttttg ccgcatcaaa tcccgccctc
    21721 agtctgaaaa attcaaatgg tttatattct ttgtcacgac cgacattatt tccataaaca
    21781 agtaaatact ctaacataaa atgattcttg tcaaaattca cagtttcact ggcactgata
    21841 cctgcatatc ccacattaag acggctggtc acagggaaaa catcattcac atttgtaggc
    21901 gtatgtctga acatatcgcc gcttacaagt ctgttaaatc ctcttgccgg atttaatatt
    21961 gctgacacta cttctctcat agttctgtta gcacctcttg tactttcatc aagtataagt
    22021 gaagtcaatc tgtaagtcat ttcaccaacc attaccccac caaaggtagt ctgaataata
    22081 tcattggtct gcggcggatc agtttccata aacatttccc acatcagact tcctccaaaa
    22141 gcaaacggaa ctgattccca gtatgagaat cctgttgacc ttgcagtatt aaaatatata
    22201 ctcccgtgaa acggatgtcc aaactgattt acgacgaaaa catcatcatc ccatacaaat
    22261 cctgtgtcga gattatgctt aacagatttg aaagtgatct ttgcaaaaga tgctccaccg
    22321 acatagttat taaaaagtgc taaacctata ttcatccccg ctgattcata gattggtatc
    22381 atcagacttt cccttttatt atattttgga taaaatctgt gatatttagt agtgtcaaaa
    22441 taattttctg agttaaactt ttcaggctga ttcgtatatt ttaatgaaag tttattactt
    22501 tctgatctta atttttttga ttcgtcgtca taatttttta acttttcaaa agtagttttt
    22561 ttattgtcat ttaattttaa atcgatctga gagtaaatgt cagtactgtt taaaataatt
    22621 gtcagtaaaa agagtaaaat agaaaatatt tttatcataa attttattag cattttaagt
    22681 atattgcatg caaattaaca atttagatta taaaattctg tatgaaaata atttatatta
    22741 ttttgttttt cattttgaca actttttcaa atgtgtattc tcaaaaaaaa gaaaatattg
    22801 aagattcacc ttatggattt aaaaatattt cttatcttga aagtattagc cctgactgga
    22861 attataagat tactttaggt gcatggatga atgcgatgag aggtgatatt tcagcaaatc
    22921 agctttacgg atacattgat aatcaatttt ataataaata taacggcgca gatttttctt
    22981 tttatggtta ttttcaggca aaaaaaaaca gaggatcttt aacggctcag ttcacttctt
    23041 ctaatcaaag tgataaattc aattatgaca acgattcgct ttattcaaga tctataactt
    23101 cgagccgctc aatgtttatt accactaata tgtcatatga attttataga aatagtgaag
    23161 ttacttttga tctgtatggc ggagcaagat ttaattttat tacaaatctt attgaaaact
    23221 tttataacac cggaggatca gttcaggaag gtcagacaaa gttttttatt gatccgataa
    23281 ttggtactaa attcttttac atacctttta aaggtccaaa catttccaga ttatttatta
    23341 atgggaattt tgatgttggc ggatatggac tggtttcctt tttatctttt gaatcttata
    23401 ttggaacagg ttataaaata aacaaatctg taacacttaa tctcggttac agatatatcg
    23461 acgttcatta tggtactgat acttatttaa tagatgcagg aatccaggga atagagttta
    23521 ctgccactac taatttttaa taatttccga ttatattttc tatagttttt ttataaatat
    23581 ctgactcata tccattctca gaatatgagt tttttaaacc aaattcttca gacaattact
    23641 attcctgata ttctcaaaag actcaatatc tttatttaat atcaatactc aaataggtat
    23701 atttactgca ttaaataaat tttttttagc tttaaattcg aaatgagcga tacaaatatc
    23761 tccgttgatc ccagatttga aaatactctc gaatacagaa caaaactttc acagttcgaa
    23821 ggacctttag atatcttact gcattttgta aaagtcgatg atctcgatat ttacaatatt
    23881 ccaatttcaa agatcacaaa ggattttctt gaatatataa atttcatgca ggatctggat
    23941 attgagcttg caggtgaatt tctggtgatg gcatcagagc tgatgaagat caaggctcgt
    24001 atgctcatcc cacagatcga tgaagaagga aatgtggttg aagaagatcc gaggcttaca
    24061 cttgtcagaa agctcattga gtataaaaga tttaaagatg tatctgaaga tatttcagag
    24121 cttgaaagtg atcagaagaa aaaaagtttc cgtagaaatt tcgaaaatga tattaaagaa
    24181 tatgaaacca ctgaagagat agaccgaagc cttaagaatg ttactatatt taatctgatc
    24241 aaagcataca agagagtgat cagcagtatt agaaaagaag tcattcaccc gatcgagctt
    24301 ctcgatatca atcctgaaaa tcaaaaggaa tttctaatga atgaactgga tgttaaaggc
    24361 gagattgaat ttgatgatct tataaaaaat attgaagtaa agctgcaggt catttgtctg
    24421 ttccttgcga tacttcagtt ggctttggac ggaatacttg aagtaaaaat tattgataat
    24481 gatatgtcaa gatttatggt atgtaaaaga ctaaatcctg aacttatctg atggtcagca
    24541 ctgaaataaa aaatattgta gaggcattga tatttgcttc tgaggaagaa attacaacaa
    24601 gacagatcaa ggaaattaca gacagcagtg gagtaaggat ctcggttaag gatatcgagt
    24661 cttcaattga ttctcttaat gaagactacc gcacttttaa caagtcattt gaaattgtac
    24721 agatagcagg tggatattct ttttccacaa aaaaagaata tggcagattc gtaggtaaac
    24781 tttttgaaga gaagcagaaa aaaaagcttt cacaatcagc acttgagtcg ttgtccatta
    24841 ttgcttacag gcagccgatc acgcgaaatg aaattgaatt tgtcagaggc gtcaatgtgg
    24901 attatatcgt aaattcactt ctcgaaagag atctgatcaa aattcacgga agagctgata
    24961 cacccggcag gccaattctt tatgcgacta caaatacttt tctgaaagtc ctcggattga
    25021 attctcttga agatctgcca aagctgaaag aaataaatga gatcttaaaa aatcaggaga
    25081 tagaaggtat tacagaagcg gatatagaat tatttaattc tgcaaatgat cctgctttga
    25141 cacagcctaa agaaacaggt cagcttgaat tgataacgag tgaagatatc gcagatgaaa
    25201 cttctgagaa taatgattct attgaaactg aaaatgaaat tcagattaca gatgaaaaca
    25261 gagctgaaaa ttcatttgat attatagaag ataaggaagt aattaattat caaaattctc
    25321 tatcaaattt gactaaagat gaaattgagg atgagggaat aaatattgaa tacaatgata
    25381 atgagattca gaatgataat gagagtcaga atgagaatga tctagaagaa attgacaatg
    25441 tagaagtagc taatgagaat gcagaaaatg aatttaatga agtaccggta gataaggaaa
    25501 ttgatgcgga tcaaaatgtc gatgaagaaa ttgaaggaaa tgatgaactc cttgaaatta
    25561 atgaaactga tagagaacga gaagataatg agaatgacaa agatgaaata aatataaaag
    25621 atggagattc aggaaatgat atagacttaa attttgataa taaagatttt gaaaataatg
    25681 agatcaaaga tcataatgaa aatgagattc ataaagataa accaggcgat agcgattttt
    25741 taactaaaga aggcgaagag gattcactct aaatgatcag aaaaaaggga ccaggcaata
    25801 gaaaaaattc ttcaggtaaa gaccgtttca gtaaaagagg cagcggtgtc aaaaaagaat
    25861 ttccggctaa aagaaaatat tccggtaaaa aagaatttag tggcaaagat gattttactg
    25921 acaaaaaaga tttcccggat aaatcttccc caaaggaata ttatggtaaa aaagaaagca
    25981 gaggtgtcag gaaattttct gacagatctg aaaagtcttt taaaaaagat acttacggta
    26041 aaaaagataa tagtggtact aaaaaatatt ctgacagatc tgataaaaaa gattaccnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ggaaaaaaga atatagtggt
    26221 actaaaaaat attctgacag atctgataaa aaagattacc cggggaaaaa agaatatagt
    26281 ggtactaaaa aatattctga cagatctgat aaaaaggatt acccggggaa aaaagaatat
    26341 agtggtgcta aaaaatattc tgacagatct gataagtcat ttaaaaaaga tacttacggt
    26401 aaaaatgaaa atagtgaccc taaaaaatat gctcatagtg ctgataaaca aggattttac
    26461 agcaggaaag aaaacaagaa cagctcagaa tattctgata aaaaagatcg ttatggcaaa
    26521 aaggaattca aggatacaaa ggacagttca ggtaaaaaat attataaaga gaaaagtgat
    26581 ttctatggca aaaaagatta cagatcaaga aatgaacttt ccgataagca gcgttatgca
    26641 aaaaagaaaa agatcgctcc aattttaaaa gaccctgatt cgactgaagt atatgtaaaa
    26701 aaagaaggtg aagaaggatt gatccggttg aataaattca tagctgcaaa cggcattggt
    26761 tccagacgga aagtcgatga gatgatagag cagggcagag tcactgtaaa taaaaaaact
    26821 attactgaac ttggatttaa aatagatgct gagaatgaca aagtaaaagt cgacggagag
    26881 ctggtaaaga ctgatacaaa aaaagtttac atcatgatga ataaaccaaa aggtgtaatt
    26941 acttctgtaa gtgatgagaa aaaaagaact acagttatag atctgatcaa tctgaaccaa
    27001 aagatatatc cggtcggacg gctggattat aacacttcag gcatgctgct gctgacaaat
    27061 gacggagagt ttgcaaatca tttgatgagt ccgaagaata aagttcataa aaaatattat
    27121 gtagagcttt caaaaccatt agaagaaaaa cacagaatta aattatgcga agggataaaa
    27181 ctcgacaatg tcaggactgc acctgcaaaa atgaaattcc ttaaagacaa tgattacaca
    27241 agattataca tctcaatcta tgaaggcagg aacaggcaga tacacaacat gttcgagcat
    27301 tacggatatt ttgtgagaga gctgatcaga gtggaatacg gaggattgaa aatggaagga
    27361 cttccagaag gaagctggag atatctgacg gcagaagaaa ttgcaaagct taaataaatt
    27421 ttaaatagat tttaaataga ttttaaataa attccaaata gattttaaat aaattttcaa
    27481 tagattttta aattatacta aataaataat tgtcagagca aaacgattta atcaaaagaa
    27541 gattagagga attagaagag atcatagcaa agggtataaa tccttacccg cataaatttg
    27601 atgtaacagc agattcagca tctctgatca aaaactataa agatgtcgaa tccgacgaag
    27661 aaaaagaagc aagcaaaagt gagaaggtat ctgttgccgg caggatcatg gcaatccgta
    27721 aaatgggaaa ggcagcattt tttcatatca aagacggcac aggcagaatt cagatctact
    27781 taaaaaaaga tgagatcggt gatgaatctt acgatgtatt cagactgctt gacattggtg
    27841 atatagtagg agttaacgga tatttgtttc gcacaaagac aggtgaagtt tccgttcatg
    27901 ctgattctta tgttctgctc acgaaatcca ttcatcctct accggtagtt aaagaggaag
    27961 tttccgagac cggtgaaaaa ataattcacg atgcattttc agatgtagag cttcgttaca
    28021 gacaaaggta tattgatctg attgtgaatg aaaatgtcaa agatgtattt gtgaaaagga
    28081 caaagatcat tcagtcgata aagaggacac ttgagaaaaa tgatttcttt gaagtggaaa
    28141 ctccgacatt gcagtcaatt tatggaggtg caaatgcaca gccgtttgtg actcatcaca
    28201 attctttaga catagatctt tatttaagga tatcgaatga actttttcta aagagattaa
    28261 tagtcggtgg atttaatcgt gtatatgagt ttgtcagaga cttcagaaat gaaggaattg
    28321 acagaactca caatcctgaa ttcacacagg tcgaatggta tcaagcgtat ggtgattatt
    28381 ttgattcgat gaaaatgttt gagaatgtag tagagcaggc gtgcattgat gcgaacggaa
    28441 ctacgaaagt aacatacggt gaagtcgagc tggatttcaa aagtccgtgg aaaagacttc
    28501 ggatgacaga tgcaataaaa gagaagacag gacttgatgt attggagatg aagaagcagg
    28561 agataattga tcacatgaat aggaatggaa tagaattcga tccgaagatc tctcatagca
    28621 aaggacttct tatagcacat ttgtttgaag agacatgcga ggaagatctt attcagccgg
    28681 tttttatata tgactttcct atcgatacga caccgctttg taaacagctc agagagtatt
    28741 cgatgaagca gcttgatgaa atgaggaacg atcctgagca ggttattttt gctgaaagat
    28801 ttgaacctta cgtaaataaa tgggaaatgg gaaattcata tacggaatta aatgacccca
    28861 tacttcagag aaaacttctc gaagagcagg tagaacgggg acgcgcaggt gagaaagaga
    28921 ctcatccgct tgatgaagat tttttaaagg caatcgaagt aggtatgccg cctactacag
    28981 gtgtgggaat gggagtcgac agactcgtga tgctgctgac taattcacag acgatacggg
    29041 acgttatatt ttttccattg atgagaccgg ggaagagcaa tttgtaatta ggaatttaaa
    29101 actatgagtt aaatattgtt tgataggagt taatagttaa tagttaatag ttaataatta
    29161 attgttaaca gttttttatg atttggaaat aaattcattt atacatattc gaaaatcgca
    29221 gcagaaagct gtagattgcg aaggtgttgt gagtttcaaa caatatttaa gaattattca
    29281 aatgaatcaa aacacagaat tgattctata aaaagagcat cagaaccctc cccctaccaa
    29341 gtggagggca gggtggggtt g
//
LOCUS       DAORVQ010000018        28462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_573_length_28462_cov_7.006663, whole genome shotgun sequence.
ACCESSION   DAORVQ010000018 DAORVQ010000000
VERSION     DAORVQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 28462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28462
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_28462_cov_7.006663"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1872)
                     /locus_tag="PLX80_02920"
     CDS             complement(160..1872)
                     /locus_tag="PLX80_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19779.1"
                     /translation="MKTLSIYIISFIFFNGNLSAQSPEYLKGFPKDLDSYYQPMLYGA
                     TPIIANFDNQGDKEIAFALFKTNHTRIFILKSDGSDLAGWPVTIVPAHSWPAIASGDI
                     NNDGNLDIVLRNNDSLYVFNYDGTRMQGFPLFLKDVNLAFVSLYDINNNGYLELIIKG
                     FNYLYVYDHLGNIVNGWPRKIPGDPPDAVKTPPVSIADLDNDNYAEIIVNSTLCLDGF
                     NCDSTYISIFSHDGTNLPGWPLKIDSGYIFYSQPATIYNDPMNDSTFLYINSAYYFPN
                     TDSVRTKTSIYNTGGNLILNYYSTTVSSESSSIALSYENNNFYQSFGSEPGPIFLFDR
                     KNLLMNNWPVFSNGYYFNSPLIMKMNNDIIITSHIRIIDFSLKGFVYFYNKNGSQLPW
                     SPLRPSGVVSAAGIYGDINNDNQLDYFIFSLLDTNNVSHPILNGWTFPGVTYDQKNMH
                     WPMYGHDRYRTNQYGFIPPDEPVGIQPINSNVPTSIKLHQNYPNPFNPVTNIKFDIKK
                     SADVRLNIYDALGRKISELINEKLQSGSYNAVFDGSDFSSGVYFYKLETEGFVETRRM
                     ILLK"
     gene            complement(1889..2239)
                     /locus_tag="PLX80_02925"
     CDS             complement(1889..2239)
                     /locus_tag="PLX80_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19780.1"
                     /translation="MKRTFLFLAAVVLTAFMLSDIQGVENNRFPNDIRNYILYSPDDG
                     WVITAWIGTTLYGSCGPTLSNGLCGITGLPAGTYTLKATKNDCTGTQIIYHPGTGYTD
                     VDWGPDNPALDCAR"
     gene            complement(2330..2506)
                     /locus_tag="PLX80_02930"
     CDS             complement(2330..2506)
                     /locus_tag="PLX80_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19781.1"
                     /translation="MTTNSNGIITFDNGGSYFPNGSYEVKATKFGFGDGVTTLNIVNG
                     VPVYPIVSVNIGTP"
     gene            complement(2794..5736)
                     /locus_tag="PLX80_02935"
     CDS             complement(2794..5736)
                     /locus_tag="PLX80_02935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HQY19782.1"
                     /translation="MKQKIKFTNYFLLLFLFSSVFFSFRNYDDNKNSASKNYFRLDQN
                     LTELDYFPNTVIFKLKESNRNSSGIADIDNIKITNTLERLGSRGVSKMFADAVKPMND
                     QTASGEKYSDLSLIYIFNFNSAVSLEDAVNEIYNTGEVEYAQPKYIQHVSSVSSFTPN
                     DPSLGQQYFINKIAAPAGWDIQQGDTNVVIGIVDSGTDWDHPDLAANIKINYADQIDG
                     IDNDNDGYVDNYRGWDFGGVDYNNVVSDNDPMIMGANNNHGSHVSGDASAVTNNGIGV
                     AGPGFKCKIMGVKCAADNDTRGPGGLGLIITGYEGIKFAADMGCDVINCSWGGGGGGQ
                     FEQDVITYATINKNSLVVCAAGNDGSSNTFYPAAYKYVLNIASTTSTDTRSSFSNFGT
                     SIDACAPGSSIYSTLYNNSYANFSGTSMASPITAGVCGIVKSQFPTYTAMQVGEKVRV
                     TSENIDAQNPSYVGRLGKGRINMHRALTVSSPSVRMNSFSVTDGNNNVPQPNDTMNIT
                     GNFINYLDATSNVAADITTTSTAVTMMNGNSSLGAVGTLGTANNGGNPYRVRINGNAA
                     ANSKVEFKVTYTDGSYSDFEYFTVVVNPSYFSMNANKITTTINSRGNFGFNDYSSNTQ
                     GEGFKYNNGSNLLFEGGLICATAPNRVSDCVRSGDQAVQSMDFTNIIPFQITDPGILS
                     NQDGSAKYNDDAAGSSKIGVEITFNSYAFNDEQDEDYVIVKYKIKNTTGAAITNFYAG
                     LFGDWDIGASGALNRASWDAANQLGYLWRTDNNPGTYVGVALLSATNPSYWAIDNEQN
                     IAGNPWNIYDGYSDPEKFESLSSGIGRMDAGGTTGRDVANTVGSGPYTIDANSEITIT
                     FALVAGTNLADLQANTVAAKLKYNTVLGITNYNSIAPDKYSLSQNYPNPFNPSTKISF
                     GLSKAGYTSLKIYDALGKEVANLVNSNLQSGSYEVDFNAANLNSGVYFYKLQSEGFIE
                     TKKMLLVK"
     gene            complement(5813..6238)
                     /gene="ndk"
                     /locus_tag="PLX80_02940"
     CDS             complement(5813..6238)
                     /gene="ndk"
                     /locus_tag="PLX80_02940"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQY19783.1"
                     /translation="MSNSLQSTLCIIKPDAVKKKVQGSIVQMLLDAGFNIRGMKMIKL
                     DEVTAGKFYEVHKERPFYGELVSFMTSGPVVVIALEKENAVSDYRKLIGATDPAEAEE
                     GTVRKIFADSKSENAVHGSDSEENGKIETGFFFTEKELV"
     gene            complement(6280..8580)
                     /locus_tag="PLX80_02945"
     CDS             complement(6280..8580)
                     /locus_tag="PLX80_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HQY19784.1"
                     /translation="MDNNNYSEEELDKYFSDKKYRKKKTKSNSTFNGFFNTAIFIFIA
                     VIIAGGAYLFYLSKTLPSLAELENPKLEEATKIYSSDGELIDKFFLKNRTKVTLDNVP
                     KDMINALISIEDQKFYDHWGVDVDRIFKAFIKNVMSGSLTKEGASTITQQLARNLYKD
                     IGNEISINRKLREAMTAVQIERTYTKAEILAYYLNTVYFGKGAYGIEAAAQTYFNKTA
                     RDLNLDECAVLVGVLKSPSNYDPVDQPEKSIERRNLVLNEMYAEGFISKENLDIALND
                     ALKTSLKKDYATFDSSAPQFTEYVRQLMEGIAEKHGYDLYRDGLKVYTTLDSRFQKHA
                     EDAVKTQLSTFQKSFNNYWSWNSTQNKTILKDAISKNIRFSEEYKKAKTEEERVEIAN
                     RLKADKQYIDSVKKLTSTVQVGFVVMNPKTGEIKAMVGSNPNSVFKYGLNHVTQIKRQ
                     PGSAFKPFVYAEAIINGYSPGYQISNDPLVVNIGGTVWSPKGGGTGGSVSLRDALAKS
                     INVVAARTAMELAPIDKVIELSHKMGINSELPNVISLSLGVGEVSPLEMTNAYGTFAN
                     EGIWVEPIAILKIEDRNGKIIEEFHPQTREAMSEEAAFIMCDMMQGVIDYGTATSVRQ
                     FFQRPAAGKTGTSQNYTDAWFIGFTPQFVAGVWLGFDDARVKFGGSYGQGGTAAAPIW
                     GRFMGKLYSDDQFDFPIAYFLMPETVDEGAVCSVTGMRSNGSCPTVNDLSIKKNSQKK
                     CNIYHYSAPTETTGETSDPPAGSVGF"
     gene            complement(8584..9351)
                     /locus_tag="PLX80_02950"
     CDS             complement(8584..9351)
                     /locus_tag="PLX80_02950"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQY19785.1"
                     /translation="MSGAYYFFSDVHLGLESDEKEKLKEIKLTEFLHSIQEDAKEIFI
                     VGDLFDCWIEYKYVVPKGYYRLFTKISELTEKGIKINYIAGNHDFWKGDFFKKEFGIE
                     ICHTHIEREIEGKKFFIHHGDGFAHNDTGYRILKKILRNKFSQKLYSMLHPSIGIRLA
                     KGTSETSRKHTSVKDYSEKDGLRDRALEMLENGFDYVVMGHRHRPVMIEHSGGYYINL
                     GDWIDHFSYGIYENGNFELKTFYDLNYKGLENKKHNQ"
     gene            complement(9369..9812)
                     /locus_tag="PLX80_02955"
     CDS             complement(9369..9812)
                     /locus_tag="PLX80_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19786.1"
                     /translation="MKYIKKTAFFIAVLMIIPFMGMNISDINSAAPGVSASVNKKSYS
                     PGETGTITLTFKTGSKVKIPKDPGVTMDLTTGEIEGVGFQDYSGGDGDYISNSKVKYE
                     FKVPDNAASGSTISVTGSVKFGYCTVSDGVCKMANQKFSTKVKVK"
     gene            complement(10038..10886)
                     /gene="folE2"
                     /locus_tag="PLX80_02960"
     CDS             complement(10038..10886)
                     /gene="folE2"
                     /locus_tag="PLX80_02960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HQY19787.1"
                     /translation="MSKVSTASVVKLPDVQNQTDIREIPIDKVGVKNLKYPIAVKDRD
                     NELQHTIATVSMTVDLPKEFKGTHMSRFVEILQNEDREIHVDSIDSLLLKMQKRLNAY
                     SSHIEIEFPYFKMKNAPVSGKPSLIDYTAKFHAVRSGKKKDFVLTVKVPVTTLCPCSK
                     NISKYGAHNQRGEVTVSVRFRNTVWIEDLITIVESSASSELYSLLKREDERFVTERAY
                     ENPVFVEDLVRNVVMKLKKKKNIIWYSVEAENFESIHNHNAYAHIVSHKNFMNGSESD
                     HLIRRY"
     gene            complement(10980..11456)
                     /locus_tag="PLX80_02965"
     CDS             complement(10980..11456)
                     /locus_tag="PLX80_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQY19788.1"
                     /translation="MKLATLLYIQNESGEYLLMERLKNPNMGLISPPGGKVETQLAES
                     PVYCAVREAKEECDLDSDENDWTLAGIVTEREYPHIGNVMIFLMEYKHRIKYLPDNCY
                     EGNFFFIHPDNFDKHNIPETDKKFIWSRIFKKRNEPFFISLDCREFPEIKEINCHI"
     gene            complement(11469..13373)
                     /locus_tag="PLX80_02970"
     CDS             complement(11469..13373)
                     /locus_tag="PLX80_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY19789.1"
                     /translation="MQDKFTVIKNGLVLTLDKHSAAGYFTIILKNGKIFLIDCENKFS
                     EKEFISKNPDAVIIDAKDKLIMPGLFNSALNSSFSLCKHFLDKCNYENLRSWHSLKLV
                     ENFISDNYNTEIMSDILNISYNSALKNGEVFINESSNCISKSILEKSYLQNHISKQYH
                     NLTAFDQNYPSSGFDDDKISNTLSLGLIADEELNNYSLSSLKKSVSQLNARLFVEASV
                     SQSVIESVKKIFGKPLITVLSEMELVNSNTVVYNPTHINQIELDILKKKNASILISPS
                     DYLNLAGRKIDIDELLFSGLNIIIGTGYTGSNILTELKVLSNIIPKSSLSYGSVFKMA
                     THNPSRLFGLSNVTGSIERNNSADMIFFSLNDLRNTVSIPEITGESLCEFVIKNLTIK
                     DISNVIIKGEQLVMDNRLSGVNEQELIIKAVAVSDKLYKAGKFIEYKEKYLMRERVNK
                     ISLSGNNESEDGEIRKSSKEIYVDMKEMDTYEGEGEFSIKGKKEEEFEKSREAKTEQN
                     DKIDGILEIKDSGMEMDLISESDKLEQLEQISRHNKKIQKTEKQFTTEKPVAKETAMI
                     SEIKEDIRSEAVNSKIVFDDIGEIEEIDREIDREIEKENTDEVENEITNDSKPAFKKE
                     KLKFGFRDGE"
     gene            complement(13406..14365)
                     /locus_tag="PLX80_02975"
     CDS             complement(13406..14365)
                     /locus_tag="PLX80_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HQY19790.1"
                     /translation="MDPVVISTWKHGLAANAEAYKILKEGGNSLDAVEMGVKISEDDP
                     DVLSVGYGGLPDCEGSVTLDAAIMDWRARIGSVISLENIKNPISLARLVLENTEHTIL
                     AGDGAYDFALKNGFKPESLLTENSIRRYHEWKASGGRKAEEIHTDDFLIKKDIIEKIN
                     ADGNHDTIGMVAIDKDGHISASCTTSGMAWKLKGRVGDSPVIGSGLYVDGDVGGAAST
                     GRGEECIRACGSFLIVEMMRNGKSPMEACKTACERVYKLNKLSDINRDHIYQVGFVAL
                     NIKGEHGAYSVREGFQYALYKNDDNSLNNSDYFINEKFIIEDL"
     gene            complement(14433..15464)
                     /locus_tag="PLX80_02980"
     CDS             complement(14433..15464)
                     /locus_tag="PLX80_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQY19791.1"
                     /translation="MNHSGSIVLETFESKILKGNPLKDPYIRDFPVYLPPSYFLSPKK
                     KFPVTFLISGFTGKGIMNLNVSFLSENIHERLDRLISTGKMKEMIVVMPDCITKYGGS
                     QFINSTATGKYEDYVISELVPFIDMKYRTISDSSSRAVCGKSSGGYGAAVLAMKNPDV
                     FGLMSSTAGDMAFEYCYMPDFPVAMIGFEQFGKGHKGVSNFIKNKLNFDLPKPKNYND
                     MLNMIGMSSCYSPNPSGLKSKGYNFDLPYDVNTGELDEKVFNIWLTYDPVRMVKKYIK
                     NLKKLKLIYVDAGIRDEYNLYAGARIFCDKLKRNKIKFIHEEFDAGHMNIPFRYDRVF
                     EIISKHINS"
     gene            complement(15579..16280)
                     /gene="ispD"
                     /locus_tag="PLX80_02985"
     CDS             complement(15579..16280)
                     /gene="ispD"
                     /locus_tag="PLX80_02985"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQY19792.1"
                     /translation="MNSEVKVIIPASGSGSRFGGKTAKQFLKVDGKEILTHTIKKFHD
                     LKIIDEIIISTIPEYFVAVSTIVRKNNFTKVKKIVEGGKLRQDSVYNALINLDCNKAD
                     IILVHDAVRPLVSDTLIKNVIVDTKKFKCVIPALRISDTLMRTDKNSFAKEIIDREGV
                     VSVQTPQGFRFDILKSSFLKAYEEGFTGTDEASIVQKYGYNVKVTEGEKSNIKITVKG
                     DLDEMKNLFKKRMSQ"
     gene            complement(16516..17496)
                     /locus_tag="PLX80_02990"
     CDS             complement(16516..17496)
                     /locus_tag="PLX80_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY19793.1"
                     /translation="MKDITIIIPKIAILGSIEGPRQLFTQVNNFFIEKGRKPVFNIKL
                     AGLTKETPVSGGLYTVHADVLIKNLKKTDLIVIPAMDGDLMKAIELNKDFIPWIKKLH
                     ASGAEIASLCVGAFLLAATGIMDGKKCATHWMAAGYFRKMFPDVDLVTEKIITDEAGI
                     YTSGGAYSYLNLILYLIEKFSGREMAILCSKVFAIEIERSSQSPFSIFSGQKEHGDEP
                     IKTAQDFIENNFQEKITVDMLASLLVLGRRSFERRFKEATKDTVKSYIQRVRIEFAKK
                     CFETSTKNINEVMYDTGYSDNKAFREIFKKLTGMSPLQYKSKYNTRVYSR"
     gene            17701..18105
                     /locus_tag="PLX80_02995"
     CDS             17701..18105
                     /locus_tag="PLX80_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY19794.1"
                     /translation="MGTQMYVNLPVKDLKRSVDFFTALGFKFNSQFTDDKATCMIIGE
                     DIYVMLLTEGFFKTFTKKDINNTSENNEVILCISADSKEKVDEYINRAVTAGGTTPME
                     KQDHGWMYSNGFQDPDGHLWEVMYMDINAMPK"
     gene            18238..18726
                     /locus_tag="PLX80_03000"
     CDS             18238..18726
                     /locus_tag="PLX80_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19795.1"
                     /translation="MKTVSSRMQSLVILFDMHTDYLKRALEGISAEDSQKRLDTKANH
                     VAWITGSLVQERFEVAGIFGAELHQKAIGLFKDHKGIQEGEKYPSSQEFLTDWDMISP
                     VLREHLVNVTDEKLDEIFKMPDFEMTNYELITFMTYREANMIGQIALWRRLLGYDAMK
                     YM"
     gene            18783..19172
                     /locus_tag="PLX80_03005"
     CDS             18783..19172
                     /locus_tag="PLX80_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HQY19796.1"
                     /translation="MEKSKSKKVSKAKYVDAFVISINIKNVPAYKKMAELGREVWMEY
                     GALDYKECVGEDLDTKMGLPFPKLTGSKKTETVIFSWITYNSKAHRNKVNAKVMKDPR
                     MNNMDGKKMPFEMDKMSYGGFKVLVSS"
     gene            complement(19364..19609)
                     /locus_tag="PLX80_03010"
     CDS             complement(19364..19609)
                     /locus_tag="PLX80_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19797.1"
                     /translation="MKLELINLLSEIDSRFSGPKSRRLFEMISGKREVDYNEIERYLN
                     YLKNLGGDENDKLIQKNQQDAADFLEQFLEHRKLMKV"
     gene            19800..19988
                     /locus_tag="PLX80_03015"
     CDS             19800..19988
                     /locus_tag="PLX80_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19798.1"
                     /translation="MNIQENSDAVKLIKEIRNKLSELERMIGKKTTANYTYPMNNQQH
                     TPPRKRNVYKNSDKKNNK"
     gene            20124..20588
                     /locus_tag="PLX80_03020"
     CDS             20124..20588
                     /locus_tag="PLX80_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19799.1"
                     /translation="MKFLYTGFYLLSLAFFILALLGGNISRPLFETLSEKTIEKTGFR
                     KAYIQSVDDKIDDLIYKSKQIELQIDKIRNFFSSDKIDESKYAKEESKMLENAVYTPV
                     LNMFILMYRISLGILSIMILCFAVIFQLIYKSLDLRKRVRRLEEAMYAGNMV"
     gene            20585..21556
                     /locus_tag="PLX80_03025"
     CDS             20585..21556
                     /locus_tag="PLX80_03025"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQY19800.1"
                     /translation="MTKITTDISEAVSEIMKGKVTALPTETVYGLGANALNEEAVLNI
                     FETKDRPAFNPVIVHIHDAGEMKKYSTEIPDIVYDLAENFSPGPITFVLRKNKIIPDI
                     ATAGNDSVGLRIPSHKLFREVLKDSKLPIAAPSANRSGKISPTTAEEVLKEMDGKIDL
                     ILDGGKCDIGIESTVISFLNGEIKILRHGFVTQNEIEKITGRNSVIGTDINSEKKVSP
                     GMLSSHYAPSTALYKCDFPNRVSDITKQKTGVLNFSKYSGNKEIAFNLFSDLRELDDA
                     GYEMLLYKEVENSGIGVAINDRLKRAGSGEIIMNGSRIKIIKNKYQQ"
     gene            21557..22867
                     /locus_tag="PLX80_03030"
     CDS             21557..22867
                     /locus_tag="PLX80_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19801.1"
                     /translation="MSERIIIEFDDESKSEKDKSGKLSSGDKKSESTGQENERIEIDL
                     SDEINKIEIENIEETYEDKILRKSIDCCYKGTNGHNNFFESNLNFPEGIEKGFSQSFR
                     INLKDEFFNSVLFNNRYLILSSYTGHVYFADRFTGYVEFKIHMHEQSFEKTGLVISNV
                     VYLNSVRKIFKYAGTGEKKFDAEEIYSTVDDTFIWSNLNRRGNKILFTEYFKTEGTAE
                     LVIIDINSPEEIFRYRISVRNSISDRICISGKNAFILVDSKLFVFDIDKMTGEVSDIN
                     IPADENSFLFSSGNRLYITTISYELYYLDLPLRENIFKSTGIKNSYINSLGGFGDNVF
                     MGTMEGWKLFKSTGLKIYEFEDEYENRIECISRNVMVISKNNKIVFSNLNRFQEAEGY
                     VISSDRLNETEFIVSAVISENEIFILTRRGIVESFTNDKLNIHI"
     gene            22871..24541
                     /locus_tag="PLX80_03035"
     CDS             22871..24541
                     /locus_tag="PLX80_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19802.1"
                     /translation="MNKVRKFIRSNFKKFLFLIIIAAASDAVPQTGNQFIFLFDNSGS
                     MLPYYNSPQSNFKLFSKALIKNSAKGSDKTGIFLFTKTDQKRAINSPKMLYNGSADEM
                     LMDKIFMKFIPMRGNDNLTGTTDLIEALDKGIAAVEGNSAVIWLITDNINDNSGSGDS
                     SYSNTLEFYKKLRSDANIQKILLYPIPEKAEFEGDAVKGYVVYGIVYSKSRLSQSELE
                     SYDTILRGAGIKEKAITLKPLDIGTIVLRPQVTQSKITDGKIYFDGSSLLGKGFEEGE
                     KVQETFSDLTLKSNLYPYIIKSAKLSVGLDNFTSSDYSVKSLGTQTISPSTVSNVTPE
                     GEVTGFSVIFNLPEITPNFSFNTIFKEDFTIGGDLILEVSQVDILLDESYVNRFRELF
                     ALQSVPEIFQPVLKDKKIITKIPLEIRMKYGPWRLFVLIGLIALLLGLILFFLYVIFK
                     KKCFGLSIDNGPVQDVCLGGLSSYSVSSGMSGELGKVKKSIFGGLSFEYSKVTTTPGT
                     SVKLIENIPIEIQYEDEGFRSNSVSLMITASEAGPGTNLGDTDDSGMY"
     gene            24717..28262
                     /locus_tag="PLX80_03040"
     CDS             24717..28262
                     /locus_tag="PLX80_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19803.1"
                     /translation="MVIKKFIILIPLLLITSGLYAQQNPNYKSITTQIGNRDTLVTLP
                     EKFIIPFSEILTLQNKILTPVSDYKLDQRQGIITLSKDLFSKYELDTFQIYNLRTDYD
                     VFPYDIKEEYSNFEIITELDTITGDSVMIATQNKDFMGSLFEGTDLQKSGSLFRGVTL
                     GSNRDLSLNSGFRLQLNGKLTDDLEINAALTDETTPIQPEGNTQKLQELDNVFIELKG
                     QDFTGTIGDIKIDFVNTQFTNFSRKIQGAKGFGSYGNGLGDLMLAGAVQRGKYNTNAF
                     NGTDGIQGPYYLIGRDNELNILVLSGTESVYLDGIQMLRGEQADYTIDYGIGTITFTN
                     NRIITVASRIIVDFEYTDREYTRTLISGANNLNLLSNKLKIGFSYVNEDDNEDKPIDF
                     SLTETDKEILSQAGDNRLNAVVSGVQFVGRDSAGIGLGLYVKSDTLINTETIVYYKYL
                     PNDSNALYQVAFSFVGQAKGNYIQNSSLQYTFNGISQGSFDTVVFIPLPNSYQIADIN
                     LNYESSPQREFTMNLESAFSTFDANKFSEKDDNNNVGVALFGTIGLSKQNFNFLGTKI
                     NALELKLKSKIVNKLFVPLERYNPVEFYRQYDIQDSNKLTEDLREASLFIAPVNFVKF
                     NAQFGQLLRGDGFNSLRTSGEFVMSDDTKKIPDLFYNVEYITTDNSYINTNSVWLRQT
                     GSAGYKKYLGDFSFDSPNLELKLDFHQENRKNIVPGQLGDSLLTGSFSFYEVIPNFTI
                     NNLLDLNFYTAFKFRLDNQPINGIIQEESETFTQTYGLRYGGVQWFSTFLELSFRKKE
                     YTDEFISDINTNNNSLLVNWQTRLDPLNGFFLTDLLYNTSSEREAKTERVFVEVRVGE
                     GNYIYIGDLNANGLKEENEFQLTNFDDGNYIRINQPTAELFPVTVLNTSARITLKPAR
                     YFNISGNDFLSEVIRNFTTESYVRIEENSKNPDTDDIYFLNFSTFQNDSNTLFGTNIF
                     QQDINFFEYNTAYSLKLRYIQQQTMNQFVGGNERFFGIQKLVKLKLGLTTDITTFFEY
                     TNRVDRNDAPLNSVRNRNIKSEGISGDFSYMPIPEIESGFQLNFASALDYYPTIPTQA
                     DINTILFRIIYSFTLSGRVRAEIQRDNISINNPDAIYPYELTNGKPVGNSILWRLLFD
                     YSISKNLQATFNYDGRAESNRNIIHTGRAEIKAFF"
BASE COUNT         9130 a         5070 c         4739 g         9523 t
ORIGIN      
        1 cagacagact cttttaaggg gggaatactt atgaattatt cataatcatg ctattaaaat
       61 tacaggtttt ttattcgtaa ttcgtaattt taattgcaat tgatttatca agactttgct
      121 gtttggaaga ttcttaattt ctaattttta attctcaatt tattttagaa gaatcatccg
      181 cctcgtctct acaaatccct ccgtctcaag cttataaaaa tatacaccgc tcgaaaaatc
      241 acttccgtca aatactgcgt tgtatgagcc ggactgtagt ttttcattta tcagctcaga
      301 gatctttctg ccgagtgcat catagatatt cagccttaca tcagccgatt tcttaatgtc
      361 gaatttgata tttgtgacag gattaaaagg gtttggataa ttctgatgaa gtttaatact
      421 tgtaggaaca ttagaattta tcggctgaat gccgacaggt tcgtcgggag ggatgaagcc
      481 gtattgatta gttctgtagc ggtcgtgacc gtacataggc cagtgcatat ttttttgatc
      541 ataagtaaca ccgggaaatg tccatccatt cagtatcgga tgcgaaacat tatttgtatc
      601 aagtaatgaa aaaataaaat aatctaattg attatcatta tttatatcac cgtaaatacc
      661 tgcagcacta actacacctg aaggtcttaa aggggaccat ggtaactgac ttccattttt
      721 attataaaaa taaacaaacc cctttaatga aaaatctata attctaatgt gagaggttat
      781 tattatatca ttattcattt tcatgattaa aggagaatta aaataataac cattactaaa
      841 aactggccag ttgttcataa gtaaattttt tctatcaaat aaaaatatcg gtcctggttc
      901 acttccaaat gactgataaa aattattgtt ttcataactt aatgcaatag aactggattc
      961 actgcttaca gtagttgaat agtaatttaa aatgagattg ccgcctgtat tatatattga
     1021 agttttcgtt cttactgaat cagtatttgg aaaataataa gcggaattta tatataaaaa
     1081 tgtagaatca ttcataggat cattataaat cgtagcaggc tgtgaataaa atatatatcc
     1141 cgaatcaatt ttaagcggcc acccgggtaa atttgtaccg tcatgactga aaatacttat
     1201 atatgtcgaa tcacagttaa aaccgtcaag acataaagtt gagtttacta ttatctcggc
     1261 ataattgtca ttatcaagat cagcaatgga aacaggtgga gttttaactg catctggcgg
     1321 gtctcccggt attttacgag gccagccatt tactatattt cctaaatgat cgtacacata
     1381 caaataatta aatcctttta ttattaattc aagataaccg ttattattaa tatcatacaa
     1441 gcttacaaag gcaagattaa catctttaag aaatagcgga aatccctgca ttctggtacc
     1501 atcataatta aaaacataca gactgtcatt atttcttaat actatatcca gatttccgtc
     1561 attattaatg tctcctgatg ctattgctgg ccaggaatga gcaggaacaa ttgtaacagg
     1621 ccatccggca aggtctgacc cgtcactttt cagaataaat attcttgtat gatttgtctt
     1681 aaaaagagcg aaagcaattt ctttatctcc ctgattatca aaattagcaa ttataggagt
     1741 cgccccatat aacattggct gataataact gtcaagatct tttggaaaac cttttaaata
     1801 ttcaggtgat tgtgcagaaa gatttccatt aaaaaatatg aaacttataa tataaatact
     1861 tagagttttc atgaatttta tatttaactt atctggcaca atccaaagca ggattatcag
     1921 gtccccaatc tacatcagta tatcctgtac ccggatgata aataatttga gtaccggtgc
     1981 aatcgttttt tgttgctttt aaagtatatg ttcctgctgg taatcctgta ataccacata
     2041 atccgtttga taaagtaggt ccgcatgaac cataaagtgt agttccaatc catgcagtta
     2101 taacccaccc atcatcaggt gaatatagaa tgtaatttct tatatcattt ggaaaacgat
     2161 tgttttccac tccctgaata tctgataaca taaatgctgt aaggactaca gcagctaaga
     2221 ataaaaatgt gcgtttcatt ttaaaactcc ttctaattta attgttaaaa atatttttgg
     2281 aactattctg tttaaaaaaa atacaaaata aaatttaaaa attaaactat tagggagttc
     2341 caatattaac actgacaatc ggataaactg gaacaccatt tacaatattc aaagttgtaa
     2401 ctccatctcc aaaaccaaat ttagtagctt taacttcata acttccatta ggaaaataac
     2461 ttccaccatt atcgaaagtt ataattccat tactatttgt tgtcatcgga gttccaaata
     2521 aattacttcc tcatccgttt aatttaatag taacagatgc tccttccact aaaccggttc
     2581 tatattcgaa atgaactgtt actgatccag aaggtaattc tgagatttct gagtttgaaa
     2641 caggtcaaaa tgaaaacaag aattttgttg cgaaatatgt atttaaaaaa atcagccgaa
     2701 acaattgtcc cggctgatta atatttatac tgtatatcaa attttgttaa atttaaaata
     2761 ttaatttatt aattaacatt tctaatttta aatttacttg acgagcagca ttttcttagt
     2821 ctcaataaat ccctctgact gaagtttgta aaaataaact ccgctgttaa gattagctgc
     2881 attaaaatct acttcataag aacctgactg taaattcgag tttactaaat ttgctacttc
     2941 ttttccgaga gcgtcataga tcttaagact tgtatatccg gcttttgata atccgaatga
     3001 gatctttgtg cttggattga aggggtttgg ataattttgt gataaagaat atttatcagg
     3061 agctattgaa ttataatttg tgataccaag aactgtatta tatttcagct tggcagcaac
     3121 tgtatttgcc tgaagatctg caaggttagt tcctgctaca agcgcaaaag taatagtgat
     3181 ctcggaattt gcatcaatag tataaggacc gctgccgact gtatttgcta catctcttcc
     3241 tgtagttccg ccggcatcca ttctgcctat accagaagaa agtgattcaa atttttcagg
     3301 atcagaataa ccgtcataaa tattccacgg atttcctgca atgttttgtt cgttatcgat
     3361 cgcccagtat gatggattcg tagctgacag aagcgcaaca cctacataag ttccagggtt
     3421 gttatctgtt ctccataaat atccgagctg atttgctgca tcccagcttg ctcggttaag
     3481 tgcaccgctt gctccgatat cccagtcacc aaataatcct gcatagaaat tcgtgatagc
     3541 agctccggta gtattcttaa ttttatattt cacaataaca taatcttcat cctgctcatc
     3601 attaaaagcg taactgttga atgtaatttc aacacctatc ttactagaac ccgctgcatc
     3661 gtcattatat ttcgcacttc cgtcctgatt agaaagaatt cccggatcag tgatctgaaa
     3721 cggaatgatg tttgtgaagt ccatgctctg cactgcctga tcgcctgacc ttacacaatc
     3781 tgagactcta tttggtgctg tagcgcagat aagtccgcct tcaaataaaa gattacttcc
     3841 gttgttatat ttaaaacctt caccctgagt attgcttgaa taatcattaa atccaaaatt
     3901 accacggctg tttatcgtcg ttgtaatttt atttgcattc atgctaaagt aacttggatt
     3961 tactacaact gtaaaatatt caaaatctga atatgaaccg tctgtataag ttactttaaa
     4021 ctctactttt gaattggctg cagcattacc gtttatcctt actctgtaag gatttccgcc
     4081 gttgtttgca gtacctagag ttccgaccgc tccaagtgaa gaatttccgt tcatcattgt
     4141 aactgctgta gaagttgtag taatatctgc tgcaacattg gaagttgcat caagataatt
     4201 tataaaattt ccagtgatat tcatagtgtc atttggctga ggcacattgt tatttccgtc
     4261 ggtgactgaa aatgaattca ttctgactga aggagaactt actgttagag ctctgtgcat
     4321 attgatcctg cccttaccga gcctgccaac gtaagatgga ttctgtgcat cgatgttttc
     4381 tgaagtaact ctgacttttt caccaacctg cattgctgta taagtcggga actgagattt
     4441 tacaattccg catacacctg cagtgatcgg tgaagccatt gatgttccgc tgaagtttgc
     4501 ataagaatta ttataaagtg tgctgtaaat acttgatccc ggagcacatg catcgataga
     4561 agttccgaaa tttgaaaaag aagatcttgt atcagtggaa gtagtggaag caatatttaa
     4621 gacatattta taagctgcag gataaaatgt attactggag ccgtcattac ctgcggcaca
     4681 gacgactaag gaatttttat ttatagttgc ataagtaatt acatcctgtt cgaactgacc
     4741 gccgccaccg ccgccccatg agcagttaat tacgtcgcag cccatatcag ccgcaaattt
     4801 aattccttca taaccggtaa taataagtcc taatcctccc ggtcctcttg tatcattatc
     4861 ggctgcacat ttcacaccca ttattttaca tttaaatccg ggtcctgcta cgccgatacc
     4921 gttatttgta acagcagaag catcacctga tacatgcgaa ccgtgattat tatttgcacc
     4981 catgatcata ggatcattgt ccgaaactac attattataa tcaacacctc caaaatccca
     5041 tcctctgtaa ttatctacat agccgtcatt gtcattatca ataccatcta tctgatcagc
     5101 ataattaatt ttaatattcg ctgcaagatc gggatgatcc cagtctgttc ctgagtcaac
     5161 aattccgatc acaacgtttg tatcaccctg ttgaatgtcc catcctgcag gtgcagcaat
     5221 tttatttata aaatactgct gtccgagtga aggatcgttt ggagtgaatg aagacacgga
     5281 agacacatgc tgaatatatt tcggctgtgc atattcgact tcgcctgtat tgtaaatttc
     5341 attcactgca tcttcaagac tcaccgctga attaaaattg aaaatataga tcagagacag
     5401 gtcagaatat ttctcaccgg aagctgtttg atcgttcatc ggctttacag catcagcaaa
     5461 catttttgaa acgcctcttg agccgagtct ttcaagagtg tttgtgatct ttatgttatc
     5521 tatgtctgca attcctgaag aatttctgtt ggattctttc agtttaaaaa ttactgtatt
     5581 tggaaagtaa tcaagctcag tcagattctg atccagtctg aaataatttt ttgaagctga
     5641 atttttatta tcatcataat ttctgaaaga aaagaagacc gatgaaaata aaaaaagcag
     5701 taagaaataa tttgtaaatt taattttctg tttcatgcat tttaaaatgt taattaagaa
     5761 atggttattt gatttttttg actaattaga ctaattagac taatttcact aattagacta
     5821 attctttttc cgtaaagaag aatcctgttt cgatctttcc attctcttcg gaatcagaac
     5881 cgtgaactgc attctctgat tttgaatctg caaatatttt tcttactgta ccttcctctg
     5941 cttctgcagg atcagtagca ccaattagtt ttctgtagtc agaaactgca ttttcttttt
     6001 ccagtgctat tactactaca ggacctgatg tcataaatga aactagttca ccatagaaag
     6061 gtctttcttt atgaacttca taaaacttcc ctgcagtcac ttcatctaat tttatcattt
     6121 tcattccgcg gatattaaat cctgcatcga gtaacatttg tacaatgctt ccctgtactt
     6181 tcttttttac tgcatcgggt ttaatgatgc atagtgtgct ttgtagcgag tttgacaaat
     6241 tttatttcct ttctgtattt attgattaat atagtttttt taaaatccta cgctgcctgc
     6301 gggcgggtcg gatgtttcac cggttgtttc agttggagcg ctgtagtgat aaatgttaca
     6361 ttttttctga ctattctttt ttatagaaag atcatttact gtcggacacg aaccgttgct
     6421 tctcattcct gttaccgaac atacggcacc ttcatctact gtttcaggca tcaggaaata
     6481 tgcgatcgga aaatcaaatt gatcatctga gtaaagctta cccatgaatc ttccccaaat
     6541 aggagctgct gccgtaccac cctgtccgta tgatccgccg aactttactc ttgcatcatc
     6601 aaatccaagc cagacacctg ctacgaactg cggagtaaat ccaataaacc atgcatctgt
     6661 ataattctgc gatgtaccag tcttacctgc tgcgggtctt tgaaaaaatt gtctgacgct
     6721 ggttgcagtt ccataatcta tcacaccctg catcatatcg cacattataa atgctgcttc
     6781 ttcgctcatt gcttctcttg tctgcggatg aaattcttcg atgatcttac cattcctgtc
     6841 ttctattttc aaaattgcaa tcggttctac ccagatacct tcgttagcga aagtaccata
     6901 tgcatttgtc atttccaagg gactgacttc accaactccc agtgaaagag agatcacatt
     6961 cggcagttca gaattgattc ccattttatg agaaagttca atgactttat caatcggagc
     7021 aagttccata gcagttcttg cagctacaac gttgatagat tttgcaagtg catcccgaag
     7081 agagacactt ccgccggtgc cgccgccttt aggtgaccag accgttccgc caatatttac
     7141 aacaagagga tcattagaga tctgataacc gggagaatat ccgtttataa ttgcttcggc
     7201 atatacaaaa ggtttgaatg cagatccggg ctgacgcttt atctgagtga cgtgatttag
     7261 tccgtattta aaaactgaat taggatttga gccgaccatt gcttttattt caccggtctt
     7321 tggattcatt actacaaatc ctacctgtac agttgaagta agttttttaa cagagtctat
     7381 atattgttta tcagctttta acctgtttgc aatttcaacc ctttcttctt ctgtcttcgc
     7441 ttttttatac tcttcagaaa acctaatatt tttcgaaatt gcatctttga gaatagtttt
     7501 attctgagtt gaattccagc tccagtaatt attaaaagat ttctgaaacg tgcttagctg
     7561 agtctttaca gcatcttctg catgtttttg aaatcgtgaa tctaaagtag tataaacttt
     7621 taatccatcc ctgtaaagat catatccgtg tttttctgca atgccttcca ttagctgtct
     7681 gacatactca gtaaactgag gcgcagaaga atcaaatgtc gcataatctt ttttcagtga
     7741 agttttgagc gcatcgttta aagctatatc aagattttct tttgaaataa aaccctcggc
     7801 atacatttca ttaagtacaa gatttcttct ttcaattgat ttctcaggct gatctaccgg
     7861 atcataattt gatggtgatt ttaaaacacc tacaagtaca gcacattcat caaggttcag
     7921 gtctctggca gttttgttaa aataagtctg agcagccgcc tctataccat aagcaccttt
     7981 cccgaaataa actgtgttca gataatatgc aagtatttct gctttagtat aagttctttc
     8041 gatctggaca gcagtcattg cttccctgag ttttctgttt atggaaattt cattcccgat
     8101 atctttatac aaatttcttg caagctgctg tgtaatggtc gaagctcctt cttttgtaag
     8161 acttccactc attacattct ttataaaagc tttgaatatt ctgtccacat ccactcccca
     8221 gtgatcataa aatttttgat cctcaattga gatcaaagca tttatcatat ctttgggaac
     8281 attgtcaaga gtgacctttg tcctgttctt aagaaaaaat ttatctataa gctcgccatc
     8341 tgaagaataa atctttgtag cttcttcaag ttttggattc tcaagctctg caagtgaagg
     8401 aagggttttt gaaagataaa ataagtaagc acccccggct ataatgacag caataaatat
     8461 aaatattgct gtattaaaaa atccattgaa ggtactgtta ctttttgttt ttttctttct
     8521 gtatttttta tccgaaaaat atttatctaa ctcttcttcg gaataattat tattgtccaa
     8581 aatttattga ttgtgctttt tattttctag ccccttataa ttaaggtcat aaaaggtttt
     8641 tagttcaaag tttccgtttt catatatacc atatgaaaaa tgatcgatcc agtctccaag
     8701 attaatataa tatccgcctg aatgttcgat cataaccggt ctgtgtctgt gtcccattac
     8761 tacataatca aaaccattct caagcatttc aagagctctg tctctgagac cgtctttttc
     8821 ggaatagtct ttaacactcg tatgctttct ggaagtttcc gatgtgcctt ttgcaagtct
     8881 aatgcctata gaaggatgca gcattgaata tagtttctga ctgaacttat ttctgagaat
     8941 cttcttcagt atccggtaac cggtgtcatt atgagcaaaa ccgtcaccat gatgtataaa
     9001 aaacttttta ccttcgatct ccctctcaat gtgagtatgg cagatctcta ttccaaattc
     9061 ctttttgaaa aagtctcctt tccagaaatc atgattaccg gcaatgtaat tgatctttat
     9121 acctttctca gttaattcag atatttttgt aaaaagtcgg taataaccct ttggcactac
     9181 gtatttatat tctatccaac agtcaaaaag atctccgact atgaagatct ctttggcatc
     9241 ttcctgaatg ctgtgtaaaa attcagtgag tttgatctct ttcagctttt ctttttcgtc
     9301 agattctaaa ccaagatgta cgtcagaaaa gaaataatat gcaccactca tttatttaaa
     9361 gtaaaatact attttacttt gacctttgtg gaaaatttct gattagccat tttacaaaca
     9421 ccgtctgaaa cagtacagta tccgaatttt acactacctg ttacgcttat tgtggaaccg
     9481 cttgctgcgt tatccggtac tttgaattca tattttactt tggaattact gatataatct
     9541 ccatcacctc cggaataatc ctgaaatccg actccttcta tttcacctgt ggtcagatcc
     9601 attgttactc ccggatcttt cgggatcttt acttttgatc ctgtcttgaa tgtaagtgtg
     9661 attgtacctg tttctcccgg actgtaagat tttttattta cacttgcact cactcccgga
     9721 gcagcagaat taatatcaga aatattcatt cccataaaag gaataatcat taatactgca
     9781 ataaaaaatg ctgttttttt tatgtatttc aatgttttct cctgtttaga ttaattaaaa
     9841 atatactcat tttgattttt tatacaaaga caaattcaat atcaaatttt aaaatttata
     9901 ttttaaataa cgattactta ggtgttattg taacttttta tttataaatg ataaattgaa
     9961 cctgtttact cacatcttgt tcctgtttaa ctcgcaaaat attatttaat atttaaaatt
    10021 tattatttaa aatttactta gtatctcctt atcaaatgat cactctcact gccattcata
    10081 aaatttttat gtgacactat atgtgcataa gcattatgat tatgaatact ctcaaaattt
    10141 tccgcttcca cactgtacca gataatgttc ttcttctttt ttaatttcat cacaacattc
    10201 cgcactaggt cttctacgaa cacaggattt tcatatgctc tctcggttac aaatctttca
    10261 tcttctcttt tcaaaagcga atataattca gaactggcgc tggattcaac aattgtgatc
    10321 aggtcttcta tccatacagt attcctgaat cttactgaaa cagtaacttc acctctctga
    10381 ttatgagcac cgtattttga gatatttttg gaacatgggc aaagcgttgt aacaggaact
    10441 tttactgtta atacaaaatc ttttttcttt cctgatctga cagcgtgaaa cttagctgta
    10501 taatcgatca aagaaggttt acctgaaaca ggtgcattct tcattttgaa atatggaaat
    10561 tcgatttcta tgtgagatga atatgcatta agtctcttct gcattttgag aagtaatgaa
    10621 tctatagaat cgacatgaat ttcacggtct tcattctgaa gaatctcaac aaacctgctc
    10681 atatgagttc ctttgaattc tttcggcaga tctacagtca ttgaaaccgt agcaattgta
    10741 tgctgcaatt cattatctct gtcctttacg gctataggat acttcagatt tttaacgccg
    10801 actttatcaa ttggtatctc tctgatatct gtctgatttt gtacatcggg aagttttaca
    10861 acagaagctg tagaaacttt actcaaatta ttcctttctt ataaatattg ttgattatca
    10921 aaaatatatt tttatataaa aagacacggt ttttctaacg tgaagaccag gtttaaaatt
    10981 cagatatggc aattaatttc ttttatttca ggaaattctc tgcagtctaa agaaataaaa
    11041 aatggttcat ttcttttttt aaatattctg ctccatataa attttttatc cgtttcagga
    11101 atattatgtt tgtcaaaatt gtcagggtgt atgaaaaaaa aattaccttc ataacagtta
    11161 tccggtaaat attttattct gtgtttatat tccattagaa atatcattac gtttccaata
    11221 tggggatatt ccctttctgt aacaattccc gctaatgtcc agtcattttc atctgagtca
    11281 agatcacatt cttctttagc ttctcttact gcgcaatata caggtgattc tgcaagctgt
    11341 gtttctactt ttccgccagg gggtgaaatg agtcccatat ttgggttttt cagtctttcc
    11401 atcaaaagat attcacctga ttcgttttga atatataata gagtagcgag tttcattcaa
    11461 tataattttt attcaccgtc acggaatccg aatttaagct tctctttttt aaatgcaggc
    11521 tttgaatcat tagtaatttc attttcaact tcgtctgtat tttccttctc aatttccctg
    11581 tcaatttctc tatcaatttc ttcgatctct cctatatcat caaaaactat ttttgaattg
    11641 acggcttcag atctgatatc ctccttgatc tctgagatca ttgcagtttc tttagccaca
    11701 ggtttttctg tagtaaattg tttttctgtt ttctgaattt tcttattgtg cctggaaatt
    11761 tgctcgagct gctccaattt atcagattct gagatcagat ccatttccat acctgaatct
    11821 tttatttcaa gtatcccatc aattttatca ttttgttcag tctttgcttc tctcgatttt
    11881 tcaaactctt cttctttctt tccctttatg gaaaactcgc cttctccttc ataggtatcc
    11941 atctctttca tatctacata aatttctttg ctgctctttc tgatttcacc atcctctgac
    12001 tcattatttc cgctaagaga aattttatta actctttctc tcatgagata tttctctttg
    12061 tactctatga attttcccgc tttatataat ttatccgaga cggcaactgc tttgattata
    12121 agctcctgct cgtttacgcc gctaagtctg ttatccatta ctaactgctc accttttata
    12181 attacatttg aaatatcttt tatagtgaga ttttttataa caaattcaca cagactttca
    12241 cctgtgatct caggaattga aactgtattt ctcagatcat tcaaagaaaa gaatatcata
    12301 tctgctgaat tatttctctc tatcgaacct gtaacatttg ataatccgaa taaccgggat
    12361 ggattatgcg ttgccatctt aaatactgat ccgtatgata atgaactctt cggtattata
    12421 tttgacaaga ctttaagttc agttaaaata ttcgaaccgg tataacctgt tccaataatt
    12481 atattcagcc ccgaaaaaag cagttcgtca atatcaattt tcctaccggc taaattcaga
    12541 tagtcagatg gtgatataag aatagaagca tttttctttt tcagaatgtc cagctctatc
    12601 tgatttatat gagtaggatt gtaaaccact gtgttggaat tcacaagctc catttcagac
    12661 aatacagtaa ttaatggttt accgaatatt ttttttacag actcgatcac actttgagaa
    12721 accgaagctt ccacaaataa tcgtgcatta agctgtgaca ctgatttttt taatgaagaa
    12781 agtgaataat tatttaattc ttcgtctgct attaatccca gtgaaagtgt atttgaaatt
    12841 ttgtcatcat caaaacctga tgacggataa ttctgatcaa aagcagtgag gttgtgatat
    12901 tgtttactaa tgtgattctg cagatatgat ttctctaaaa ttgatttgct gatacaatta
    12961 ctgctttcat tgatgaatac ttctccgttt ttcaatgcac tgttataact tatattaaga
    13021 atatcagaca ttatctcagt gttataatta tctgaaataa aattttccac gagcttaaga
    13081 gaatgccatg atctcagatt ttcataatta cacttatcaa gaaaatgctt acagagagag
    13141 aaggatgagt ttaatgctga gttaaaaagt cccggcatta taagtttatc cttggcatca
    13201 atgataactg cgtccggatt ttttgaaata aattcctttt cactaaattt gttttcacaa
    13261 tctattaaaa agatcttacc attctttaaa ataattgtaa agtaacctgc agcagaatgc
    13321 ttatcaagtg taagcactaa tccgtttttg ataactgtaa atttatcctg catattatta
    13381 actatttttt cctgaactaa tttaatcaca aatcttctat aataaatttt tcattaataa
    13441 aataatcact gttatttaac gagttgtcat catttttata caacgcatac tgaaaacctt
    13501 ctcttacact atatgctccg tgttcgcctt ttatattgag cgcgacaaaa ccaacctgat
    13561 aaatatgatc tctgttaata tcagaaagtt tattcagttt gtaaactctt tcgcatgctg
    13621 ttttgcaggc ttccattggt gattttccat ttcgcatcat ttccactatt aaaaaacttc
    13681 cgcaagctct gatacattct tcgcctcttc ctgtcgatgc tgcacctccg acatcaccgt
    13741 ccacataaag acctgaccct atgacaggac tgtctccgac tctgcctttg agtttccatg
    13801 ccataccgct ggtagtacat gatgccgaaa tatgcccgtc tttatctatc gccaccattc
    13861 cgatcgtgtc atggttcccg tctgcattga tcttttctat tatatctttt tttataagaa
    13921 aatcatctgt atgaatctct tcagcttttc tgccgccgga tgctttccat tcatgatatc
    13981 ttctgataga attttccgtc agcagactct caggtttgaa tccattcttc aaagcaaaat
    14041 cataagcacc gtctcctgca agtattgtgt gttcggtatt ttctaaaacg agtctcgcaa
    14101 gacttatagg atttttaata ttctcaagac taatgacaga acctatcctt gcacgccagt
    14161 ccatgatagc agcatcaagt gtgacgcttc cctcacagtc aggaagtccg ccgtatccga
    14221 cactcaaaac atcaggatca tcctcggaaa tctttactcc catctcgaca gcatcaagag
    14281 aattgccgcc ctctttaagg atcttatatg cttctgcgtt tgcagctaga ccgtgtttcc
    14341 atgtagagat tacaacagga tccatgtgtt taattattaa tttttaatgt actatgttta
    14401 atgtgcaatt tgcaatgttt aatgtataac tattaactat taatgtgttt tgaaattatt
    14461 tcaaaaactc tgtcataccg gaatggaatg ttcatgtgtc cagcatcaaa ttcctcatgt
    14521 ataaatttta tcttatttct tttcagctta tcacaaaata tccttgcacc tgcatacaga
    14581 ttgtattcat ccctgatacc tgcatcgaca tagatcagtt tgagtttctt taaatttttt
    14641 atatactttt ttaccattct tacaggatca taggtaagcc atatattaaa aactttctca
    14701 tccagttcac ctgtattcac atcataaggc agatcaaaat tataaccttt tgatttaagt
    14761 cctgaaggat ttggagaata gcagcttgac ataccgatca tgttcagcat gtcgttataa
    14821 ttctttggct ttggcagatc gaaattaagt ttattcttta taaaatttga aacgccttta
    14881 tgaccttttc caaactgttc aaacccaatc atagcaactg gaaagtcagg catgtagcag
    14941 tattcaaaag ccatgtcgcc tgccgtagaa ctcatcagtc caaacacatc aggatttttc
    15001 attgcaagta ccgcggctcc gtatccgccg ctggactttc cgcatacagc acggctgctg
    15061 ctgtcggaaa ttgttcgata tttcatatct ataaaaggaa cgagttcgct gattacataa
    15121 tcttcatatt tacctgtagc tgtagaattt ataaactgac ttccgccgta ctttgtgata
    15181 cagtcaggca tcacgactat catctctttc atcttacctg ttgagatcag tctgtcgaga
    15241 cgttcatgaa tattttcaga aagaaaggaa acgttcagat tcatgatccc ttttcctgta
    15301 aagcctgata tgagaaatgt taccggaaat ttcttttttg gtgataaaaa atatgaaggc
    15361 ggcagataga caggaaagtc tctgatatac ggatccttta gaggattgcc tttcagaatc
    15421 ttactttcaa acgtttccag tacgatcgaa cctgaatgat tcattaattt gtaaattaat
    15481 aatgatgtgt tattccgtta ttgcttagat attattttca aagtagattc gtaaagatct
    15541 aactatttat ttacttctta aaaattcaat tcacctgttt attgactcat acgtttctta
    15601 aataaatttt tcatctcatc aagatcgcct tttactgtga tcttaatatt acttttttcg
    15661 ccttcagtta cttttacatt atatccatac ttctgtacta ttgaagcttc atctgttccg
    15721 gtaaatcctt cctcatatgc tttcaaaaaa ctactcttca atatatcaaa tctgaatccc
    15781 tgcggagtct gtaccgacac aactccttcc ctgtcaatta tttcttttgc aaatgaattt
    15841 ttgtccgtcc tcatcagcgt atcacttatt ctcaatgcag gtatgacaca tttaaatttc
    15901 ttcgtatcaa ctatcacatt ttttataagc gtatcagata cgagcggtct gacagcatcg
    15961 tgtacaagta tgatatcagc tttattgcaa tcaagattta taagtgcatt ataaactgag
    16021 tcctgtctga gttttccgcc ttccactatt ttttttactt tggtaaaatt gttttttctg
    16081 acgatagtgc tgacagctac aaagtattct ggtatagtag aaataataat ttcatcgatg
    16141 atcttcagat cgtgaaattt ttttatagta tgtgtcagaa tttccttacc gtctactttt
    16201 aaaaactgtt tggcagtctt cccgccgaat cttgatccgc tccccgaagc aggtataatg
    16261 actttgactt cactattcaa attctgaatc aaattctgaa aaggatgcgg attttttttt
    16321 aaaaataatt tcctcaaaaa taatcaatta taaatcttat tacgatataa aatttttatt
    16381 aaatgattgt aaataaattt cagctgctgc ttatacattt caaaaattat ttattattga
    16441 atttaacaaa tattttgatc gtgattcaaa atgtagcatc ataacaaacc tatctttaaa
    16501 tttattgaaa tgtcattatc ggctgtaaac tctcgtgtta tatttactct tgtattgtaa
    16561 aggcgacatt cctgttagct ttttaaaaat ttctctgaaa gctttattgt cggaatagcc
    16621 tgtatcatac attacttcat taatattctt tgtgcttgtc tcaaagcact tctttgcaaa
    16681 ttcaattctc actctttgaa tatagctttt cacagtatct tttgttgctt ctttaaatct
    16741 tctttcaaaa cttcttcttc ccagaacaag aagcgaagca agcatatcaa ctgttatctt
    16801 ttcctgaaaa ttattttcaa taaaatcctg tgccgttttt atcggctcgt ctccatgttc
    16861 cttctgaccg ctgaaaatac tgaaaggcga ctggctgctt ctttcaattt caatcgcaaa
    16921 aacctttgaa cagagtattg ccatttcgcg tccggaaaat ttttctatca gatacaatat
    16981 aagattaaga tatgagtaag cacctccgct tgtgtaaata cctgcttcat ccgtaataat
    17041 tttctcagtg acaagatcta catcaggaaa catctttctg aaatatcctg cagccatcca
    17101 gtgtgtagca catttctttc catccataat accggttgcc gccagcagaa aagctccgac
    17161 acaaagactt gcaatttcag ctccggatgc gtgcagcttt tttatccatg gaatgaaatc
    17221 tttgttcagc tcaattgctt tcatcagatc accgtccatt gcgggtatga caatgagatc
    17281 tgtcttcttt aaatttttta taagcacatc agcatgaact gtataaagac cgccgctgac
    17341 cggggtttcc tttgtaagtc ctgcaagctt aatattgaaa accggtttcc tgcctttttc
    17401 aataaaaaaa ttatttacct gagtgaaaag ctgtctcggt ccttctatac ttccgagaat
    17461 tgcaatcttt ggaattataa tggttatatc tttcataaaa tttttctttt ttatttattt
    17521 aaagaagaaa aaatataaac gataattgtc gtattcaacc ccttaataag tcgttttcac
    17581 atatatgttt taaaaatata aatatttaat tttgtataga ttaaaattta cacatcactg
    17641 tattgcaaag tcagtggtca atattataat aaaaaaatta ataaattaaa cattcttaaa
    17701 atgggtactc aaatgtatgt caatcttccg gtaaaagatc ttaagagatc tgtagatttt
    17761 tttaccgcac ttggatttaa attcaattca cagttcacag atgataaagc aacatgtatg
    17821 atcattggtg aagatattta tgtaatgctg ctcacagagg gatttttcaa aacctttaca
    17881 aaaaaagata ttaacaatac atctgaaaac aatgaagtaa ttctctgtat atctgcagac
    17941 agcaaagaaa aagtagatga atatattaac agggccgtca ctgcaggtgg cactactcct
    18001 atggaaaaac aggatcacgg ctggatgtac agcaatggat ttcaggatcc ggacggacat
    18061 ctctgggaag taatgtatat ggatataaat gctatgccga aatagttagt cgttaaaata
    18121 gttaatagtt agtagttagt agttaatatt taatatttaa tatttaatat ttaatagttg
    18181 atttaaatgt ttattgttga taaaatttta taaaccttaa ataaaataaa aatggaaatg
    18241 aaaacggtaa gttcgagaat gcagtctctt gtaattttat ttgacatgca tacagattat
    18301 ttgaagagag cacttgaagg aatttctgct gaggattctc aaaagcgatt agatacaaag
    18361 gctaatcacg ttgcatggat cacaggcagt cttgtacagg aaagatttga agttgcggga
    18421 atattcggag ctgagcttca tcagaaagcg atcgggcttt ttaaagacca caaaggaatt
    18481 caggaaggtg agaaatatcc aagttcacag gagtttttaa cagactggga catgatttcg
    18541 ccggtactta gagagcattt ggtaaatgtt acagatgaaa agcttgatga aatttttaaa
    18601 atgccggatt ttgaaatgac caattatgaa ctcattactt ttatgactta tcgtgaggct
    18661 aatatgattg gacaaattgc cttatggaga agactgctcg gttatgatgc aatgaagtat
    18721 atgtaaaaat aacacaaaat tctaaaccgt aaattatttt tgaaaattaa aaaattaata
    18781 aaatggaaaa gtcaaaatct aaaaaagtaa gtaaagcaaa atatgttgac gcatttgtaa
    18841 tttcgatcaa tataaaaaat gtgccggcat ataaaaaaat ggcagagctt ggccgagaag
    18901 tttggatgga atacggtgct ttagattaca aggaatgtgt aggtgaagat ttggatacaa
    18961 aaatgggatt acctttccct aaactaaccg gctcaaaaaa aactgaaaca gttatttttt
    19021 catggataac atacaactcc aaagctcaca gaaataaagt aaatgctaaa gtaatgaagg
    19081 atcccaggat gaataatatg gacggtaaaa aaatgccgtt tgaaatggat aaaatgtctt
    19141 acggcgggtt taaagtactt gtaagctctt agcagttagc agttaatagt taatagttaa
    19201 tagttaatag ttaatagtta atagtttgaa gttagcagtt aatagtttga agtaaatgaa
    19261 aaatgtttag attatttact tcagattcaa taatgatctg taaataaaga taaaattcgt
    19321 ccttagatat ttaaaaattt aaactaagac aaattttaaa agatcagacc ttcattaatt
    19381 ttctgtgttc taaaaactgt tcgagaaaat ctgcagcatc ctgttgattt ttttggatca
    19441 gcttatcatt ttcatcacct ccaagatttt ttaaataatt taaatatctc tcaatctcgt
    19501 tataatcaac ttctcttttt cctgagatca tttcaaaaag tcttcgtgat tttggtccgg
    19561 aaaatcttga atctatttca cttaaaagat ttattaattc aagtttcata ttcagaattt
    19621 aattattgga ttaacattta cggtacagat taacatactt tgcctctata ataaatccaa
    19681 ttataaagcc atgcacatca atctctaaaa gtttacatta aaaaaattct taaattaata
    19741 agttataatt tataaaaatt cagattaaat taattaactc atacaaacca aatttaaaaa
    19801 tgaacataca ggaaaattcc gatgctgtaa aattaataaa ggaaataaga aacaagctca
    19861 gcgaactgga gagaatgatt ggaaaaaaaa caactgcaaa ctatacttac ccaatgaaca
    19921 atcagcagca tacacctccg agaaagcgca acgtatataa aaattccgat aagaaaaata
    19981 acaaatagaa ataaatatta aggaatttct gattatctga aattcagatt ttttttctgt
    20041 actgacggat atacaagatt actttaaaac ctgataattc attatcacat atattttttt
    20101 tacaataaaa gttattttgt cagatgaaat ttttatatac aggattttac ttgctgagtc
    20161 tggcattttt tattttagct ctgttgggtg gaaatatttc ccgcccatta tttgaaacct
    20221 tatcagaaaa aaccattgag aagactggat tccgaaaagc ttacattcag tcagtagatg
    20281 ataagatcga cgatctgatc tataaatcca aacagatcga acttcagatc gacaagatca
    20341 gaaatttttt ctcttctgat aaaattgatg aaagtaagta tgcaaaagaa gagagtaaaa
    20401 tgctggagaa tgcagtctac acgcctgttc tgaatatgtt catattaatg tacagaatct
    20461 cattaggaat tttgtcaatt atgatacttt gctttgctgt tatatttcaa ctgatctaca
    20521 aaagtcttga tctgagaaaa agagtgagac gtcttgaaga agcaatgtat gcgggaaata
    20581 tggtatgaca aaaattacta cggatatttc cgaagctgtt tctgaaatta tgaaagggaa
    20641 agtaacagca ctgccgacag agacagttta cggactcggg gcaaatgcat tgaatgaaga
    20701 agctgtatta aacatttttg aaaccaaaga cagaccggca tttaatcctg tcattgttca
    20761 tattcatgat gccggtgaaa tgaaaaaata cagtactgaa attccggata ttgtatatga
    20821 ccttgcagaa aatttttccc ctggtccaat tacttttgta ctaagaaaaa ataaaataat
    20881 tccggatatt gcgacagcgg gaaatgacag cgtgggtctg agaattcctt cacataagtt
    20941 attccgtgaa gtacttaagg attctaaact accaatagct gctccttctg ccaacaggtc
    21001 aggaaagatc tcaccaacaa ctgctgaaga ggtattaaaa gaaatggacg gtaagatcga
    21061 tctcatttta gacggcggga aatgtgatat tggaattgaa tccactgtaa taagtttttt
    21121 aaacggcgaa attaagattc tgagacacgg ttttgtaacg cagaatgaaa ttgaaaaaat
    21181 cacaggaagg aattctgtca tcggaacaga cattaattcc gaaaagaagg tatctcccgg
    21241 aatgctaagc agtcactatg caccatcaac agcgttatat aaatgtgatt ttcctaacag
    21301 agtttctgat atcacaaaac agaaaacagg agttttaaat tttagtaaat attcaggaaa
    21361 taaggaaatt gctttcaatc ttttttctga tctgagagag cttgatgatg cgggatatga
    21421 aatgcttttg tataaagaag tggaaaattc aggaattggg gttgctatca acgacagact
    21481 taaaagagcc ggctcgggag agataattat gaacggcagc aggattaaaa taattaaaaa
    21541 taaatatcag cagtaaatgt ccgaacgcat tataattgaa tttgacgatg aatctaaaag
    21601 tgaaaaagat aaatccggaa agttgagttc cggagataaa aaatctgaat ccaccggaca
    21661 ggaaaatgaa aggatcgaaa tagatctcag cgatgaaata aataaaattg agattgagaa
    21721 tattgaagag acttatgaag ataagatttt aagaaaaagt attgactgct gctacaaagg
    21781 gacaaacggt cataataatt tttttgaaag caatctgaat ttcccggaag gtatagaaaa
    21841 aggattcagt cagtcattca gaataaatct taaggacgag ttttttaatt ctgtactttt
    21901 taataaccgt tatcttatac tgagttcata tacaggacat gtgtattttg cagacagatt
    21961 cacaggttat gtggaattta agattcacat gcacgagcag tcatttgaaa agactggact
    22021 tgttatttct aatgttgtat atctcaattc ggtcaggaag attttcaaat atgccggaac
    22081 aggcgaaaag aaatttgatg cggaagagat ttacagcacc gttgatgata cttttatctg
    22141 gagtaatctg aacagacgcg gaaataaaat tctatttaca gaatatttta aaacagaagg
    22201 tacagcggaa ctggtaataa tagatatcaa ttcacctgaa gaaattttcc ggtacaggat
    22261 cagcgtaaga aattctatca gcgacaggat ctgcatttca ggtaaaaatg cattcatact
    22321 tgtagattca aagttgtttg tttttgacat agataaaatg acaggtgagg tatcggatat
    22381 aaatataccg gcggatgaaa attcttttct tttttcttcg ggtaacagat tatatattac
    22441 aactataagt tatgaattat attatctgga tctgccttta agggaaaata tatttaaaag
    22501 tacgggaatt aaaaacagct atataaattc actcggggga tttggtgata atgtttttat
    22561 gggaacgatg gagggctgga aattattcaa gtcaacaggt ttgaaaatat atgaatttga
    22621 agacgaatat gagaacagga ttgagtgtat cagccgaaac gtaatggtaa tttcaaagaa
    22681 caataaaatt gtattcagta atctaaacag gtttcaggaa gctgaaggat acgttatatc
    22741 ttctgacaga ctgaatgaaa cagaattcat agtatcggca gtaatttcag aaaatgaaat
    22801 ttttattctg acgcgtcggg gcattgtaga atcttttact aatgataaat taaacataca
    22861 tatctgataa atgaacaaag tcaggaaatt tataagaagt aattttaaaa aatttttatt
    22921 tttaataatt attgcagctg catctgatgc agttcctcag acagggaatc agttcatatt
    22981 tttatttgac aattccggaa gcatgctgcc atactacaac agtcctcaga gtaattttaa
    23041 actgttcagt aaagcactca taaaaaattc cgcaaaagga agtgacaaaa cgggaatatt
    23101 tctgtttaca aagacggatc agaagagggc gatcaattct ccaaaaatgc tttacaacgg
    23161 cagtgctgat gagatgttaa tggataaaat atttatgaaa ttcattccta tgagaggtaa
    23221 tgataatctc accggtacta ctgatctgat agaggcactt gataaaggta tcgcagcagt
    23281 agaaggaaat tctgcagtga tatggctgat cacagataat ataaatgata acagcggaag
    23341 cggcgattct tcttattcca acacacttga gttttataaa aagctcaggt cagatgcaaa
    23401 tatacaaaag atactattat acccgatccc tgagaaagct gaatttgaag gtgatgctgt
    23461 aaaagggtat gtagtgtatg gtatagttta ttctaagtca cgtctgtcac agtctgagct
    23521 ggaaagttat gatacaattc tgagaggtgc gggcattaaa gaaaaagcta ttacattaaa
    23581 acctctcgac attggaacga tagtactcag acctcaggta acgcagagta agatcactga
    23641 cggaaaaatt tattttgacg gatcgtcact tctgggtaaa ggatttgaag aaggcgagaa
    23701 agttcaggag acattcagcg acctgacact gaaatcaaat ttatatcctt acattataaa
    23761 aagtgccaaa ctcagtgtag ggctggataa tttcacatca tcagactatt ccgtaaagtc
    23821 actcggcacg caaactatct ctccctctac agtaagcaac gtcacacctg agggagaggt
    23881 aacaggattt tccgtaatat ttaatctgcc cgagatcact ccgaattttt cattcaatac
    23941 aatttttaaa gaagatttta ctattggcgg tgatctgata ttagaagttt cacaggtcga
    24001 tattttactt gatgaaagtt atgtgaacag attcagagaa ctttttgctt tgcagtctgt
    24061 gcctgaaata tttcagcctg tattaaaaga taaaaagatc ataacaaaga ttccattaga
    24121 gataagaatg aaatacggtc catggagatt atttgtactt attggtttga tcgcactttt
    24181 acttggctta attttatttt tcttatatgt aatatttaag aaaaaatgct ttggactttc
    24241 gatagataac ggacctgtgc aggatgtatg ccttggagga ctttcatcat attcagtatc
    24301 ttcgggtatg tccggagaat taggaaaggt aaagaaatca atttttggcg gactaagttt
    24361 tgaatattca aaagttacga ccactcccgg aactagtgta aaactgattg aaaatatacc
    24421 aatagaaatt cagtatgagg atgaagggtt cagatcaaac tccgtatcgc ttatgataac
    24481 tgcgtctgaa gcaggtcccg gtacaaatct cggagacact gatgatagcg gcatgtatta
    24541 attttgcaaa acacaataaa tataaatttt ttaatttaat aaatttttga ttatacgttt
    24601 attaatttaa cagtgtaaat aatttaaatc tgaaaaactg tttttcaaaa agcgtaatta
    24661 cttttatgta agtaaatatc atttacaaag aaaaagcagt tgacaaaaac agagtaatgg
    24721 taatcaaaaa atttataatt ctcattccgc ttcttttaat cacatcgggt ctgtatgctc
    24781 agcagaatcc aaattataaa tctattacaa cacagattgg aaacagagac acgcttgtta
    24841 ctcttcccga aaaattcata ataccatttt cagaaattct tacattacaa aataaaattc
    24901 tgactccagt cagtgattat aagctagatc agcggcaggg cattattact ttaagcaaag
    24961 atctcttcag taaatatgag cttgatactt ttcagatata taatctgcgt acggattatg
    25021 atgtatttcc ttatgacatt aaagaagagt attctaactt tgaaatcatc acggagcttg
    25081 ataccatcac aggtgattcc gtaatgatcg caactcagaa taaagatttc atgggaagtt
    25141 tatttgaagg gacggatcta cagaaatcgg gaagtttgtt cagaggagtt acattaggtt
    25201 cgaacagaga cctatcattg aattcaggat ttcgtcttca gcttaacgga aaacttacag
    25261 acgacctgga gatcaatgct gctctgacag atgaaacaac acccattcaa ccggaaggaa
    25321 atacacaaaa gctacaagag cttgataatg ttttcatcga gcttaaagga caggatttta
    25381 caggaacgat cggagatatt aaaattgatt ttgtaaatac acagttcaca aatttttcaa
    25441 ggaagataca aggcgcaaaa ggattcggct cttatggaaa cggtctggga gatctgatgt
    25501 tggcaggtgc tgtgcagaga ggaaaatata ataccaatgc attcaacggc acggacggga
    25561 tacagggacc gtattatctg atcggacggg acaatgaact taacatactt gtattatccg
    25621 gaacggaatc tgtgtatctc gacgggattc agatgctgcg gggtgaacaa gcagattata
    25681 ctattgatta cgggatcggg acaattacat ttacaaacaa caggatcatt acagtcgcat
    25741 caagaatcat agttgatttt gaatatacag acagagaata tacacggaca cttatttcag
    25801 gagcaaacaa tctgaatctt ctaagcaata aattaaagat cggtttttct tatgtgaatg
    25861 aagatgacaa tgaagataaa ccgattgatt tttcacttac ggaaactgat aaagaaattc
    25921 tatcacaggc aggtgacaac agattaaatg ctgtagtatc gggtgttcaa tttgttggga
    25981 gggacagtgc cggtattggt ttaggtcttt atgtaaaatc agatacactt ataaatactg
    26041 agacaatagt ttattataaa tatttaccaa atgattcaaa tgctctgtat caggttgcat
    26101 tttcttttgt agggcaggca aagggaaatt acattcagaa cagttcactg caatatacat
    26161 ttaacgggat ttcacaggga agttttgata cggtagtgtt catacctttg ccaaattcat
    26221 atcagatcgc tgacataaat ttaaattatg aatcatcacc tcaaagagaa tttactatga
    26281 atcttgagtc ggcattctct acatttgatg ccaacaaatt ttcggagaaa gatgacaaca
    26341 ataatgtagg ggtggcattg tttggaacaa taggattgag caaacagaat ttcaattttc
    26401 ttggcacaaa gatcaacgca ttagaattga aactgaaaag taaaatagta aataaattat
    26461 ttgtaccatt agaaagatat aaccccgttg aattttacag acagtatgac attcaggatt
    26521 cgaataaact cacagaagat ctgagagagg catcactttt tatagctcct gttaattttg
    26581 taaaattcaa tgcacagttc ggacagcttt taagaggtga cggatttaat tcactcagga
    26641 cttcgggaga atttgtaatg agcgacgata caaaaaaaat tccggacctt ttttataatg
    26701 tagaatacat tactacagat aattcttaca taaatacaaa cagtgtttgg ctgaggcaga
    26761 caggcagtgc aggttataaa aagtatctgg gtgatttttc atttgacagt ccaaatcttg
    26821 agctgaagtt agatttccat caggagaacc gaaaaaatat tgtgccgggg caattgggag
    26881 acagtctgct aacgggaagt ttttcttttt atgaagtgat cccaaacttt actataaaca
    26941 atctccttga tcttaatttt tatacagcat tcaaattcag actggacaat cagccgataa
    27001 acggtattat acaggaagaa tccgaaacat tcactcagac ttatggattg agatacggcg
    27061 gagttcaatg gttttcgact tttctcgagt tgtcattccg caaaaaagaa tatactgacg
    27121 aattcatttc tgacataaat acaaataaca attctttgtt ggtaaactgg cagacgagat
    27181 tagatccgct gaacggtttt ttccttacag atcttttata taatacttca agcgagcggg
    27241 aagctaagac tgaaagagtg tttgtcgaag ttagagtggg agaaggtaat tacatttata
    27301 ttggtgatct gaatgccaac ggtcttaagg aagaaaatga atttcagttg acaaattttg
    27361 atgatggtaa ttatatacgg atcaatcagc cgactgctga attatttccg gttacagtcc
    27421 tgaatacttc tgcaagaatt acattaaagc ctgcaagata ttttaatatt tcgggaaatg
    27481 attttctttc agaagtaatt agaaatttta caactgaaag ttatgtccga attgaagaga
    27541 acagtaagaa tcctgataca gacgacattt attttttgaa tttcagtact tttcaaaatg
    27601 attccaatac tttattcggc acgaacattt tccagcagga cattaacttt tttgaataca
    27661 atactgcata ttcattaaag ctcagataca tacagcagca gacaatgaat caattcgtcg
    27721 gcggtaatga aagatttttt ggaattcaaa aacttgtaaa actcaaactc ggactgacca
    27781 ctgatattac aacatttttt gaatacacaa acagagtgga cagaaacgat gcgccattaa
    27841 attctgtccg caacagaaat ataaaatcag aaggtatctc tggagatttt tcatatatgc
    27901 cgattcccga aatagagagc ggctttcagc ttaattttgc atctgcactt gattactatc
    27961 cgacgattcc tacccaggct gatattaata caattctctt caggataatt tattcattta
    28021 cattatcagg gagagtcagg gcagagatac aaagggataa tataagtatt aacaatccgg
    28081 atgcaattta tccatatgaa ctcacaaacg gaaaacctgt tggcaacagt attttatgga
    28141 gacttttatt tgattacagc attagtaaaa atctgcaggc aacatttaat tatgacggaa
    28201 gagcagaaag taacaggaat atcattcata cgggacgtgc tgaaataaag gcgttttttt
    28261 agaattcacc tcacccagcc ctcaatgcaa ttaagttaag ggcgaagtat cgtcgctatc
    28321 agcaaacccc tcccagcctc ccctttgaaa aaaggaggag tctgaacttt taaattaaaa
    28381 ctttatatta atcaaattat aaataaaaaa tccctaaaat taatggcatt gctccagccc
    28441 tcccagcgag tgagtctgtc ta
//
LOCUS       DAORVQ010000019        27828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_588_length_27828_cov_6.517949, whole genome shotgun sequence.
ACCESSION   DAORVQ010000019 DAORVQ010000000
VERSION     DAORVQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 27828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27828
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_588_length_27828_cov_6.517949"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1729
                     /locus_tag="PLX80_03045"
     CDS             260..1729
                     /locus_tag="PLX80_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19804.1"
                     /translation="MKRLSRAIIIFVTALGIGIVSVSKNSLKADQSETQILNPFNLLF
                     PPTGLTFVTEPGNNTQVDITWSSAGAAATYKWKFGAPTINSVILSLSANNNGTSTALT
                     VTPAAVDGILAGLGLNPGDSVSGEWAVWAYEGNDSLKSVQTFNITFKRKVVLNQFNLV
                     SPPSGITLVTSPENNAEVNIIWKKSSAGATYKWKFGAPNINSVLLEIPSNNAGSDTML
                     TVTPAAVDGILAGLGLNPGDSVSGQWAVYAYNETDSLKSVETHNITFRRSKLASFGLL
                     FPPTGITFVSEQGNNTPVNINWNKSADGVTYKWKFGSPNVALNTILDLASNNNGMDTV
                     LTVTPAAVDGILAGLGLNPGDSVPGQWAVWAYLNGDSLKSAQTFDITFRRDNKNSISQ
                     IGSVIPDIYSLSQNYPNPFNPSTKINFGIPETGQVNISVFDMSGREVSVVLNENKSPG
                     NYEISFNGANLSSGIYYYRISVLANGKSFIDTKKMTLIK"
     gene            complement(1887..2837)
                     /locus_tag="PLX80_03050"
     CDS             complement(1887..2837)
                     /locus_tag="PLX80_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19805.1"
                     /translation="MNLFLITLTLILGSKNIYSQTDSSELRLTDKVRIAEAVRMSDEF
                     GDNVWSNFNQTLFALLLVINENEYLFYHPSPSEDFLSIGFDSITGCEIFVRERIFSNN
                     MLATFPAVNGVSTIVVGQPENTSRSSLDWTVTLLHEHFHQMQSSLPDYYESVNSLDLS
                     GGDETGMWMLNYDFPYDDQSISDQYSKLIQSAMNTYLSDDSVIFNVNLKKYIGEIEKF
                     KSLLNVKDYRYFSFQIWQKGIARYTEIKFTKILKDEYSPTESFKELSDYISIDSFYVN
                     IINKLLKSADTQILSFDKRNCFYTLGALEGLIIDKTNPDW"
     gene            complement(3015..3671)
                     /locus_tag="PLX80_03055"
     CDS             complement(3015..3671)
                     /locus_tag="PLX80_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY19806.1"
                     /translation="MAKFVSNKNETIRLFKNPFLEYFSHIHPATPLFVFIPVILATAY
                     FGSQVISLMSSIIFFVVGILLWTLMEYVIHRWAFHYHPKSEPGKRIHFLVHGIHHDYP
                     RDATRLVMPLLVSLPLATVFFFLFQAAFGEYNLIIFAGFVLGYVAYDSIHYATHHFKM
                     TGKIGKFLKVYHLKHHYGDDHTAYGVSNPLWDYVLGTVPDHVKDKSVTEISEKESPDY
                     "
     gene            complement(3765..4139)
                     /gene="mscL"
                     /locus_tag="PLX80_03060"
     CDS             complement(3765..4139)
                     /gene="mscL"
                     /locus_tag="PLX80_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQY19807.1"
                     /translation="MLKEFKEFISKGNAIDLAIGVIIGGAFGAIVSSIVADLLMPFIG
                     IIMGGIDFSGLSVAIGSAVFPYGKFIQATINFLIIAVVLFLIVKGINSMRKKEEDEAP
                     AEPTKEEHLLMEIRDLLKSGKQ"
     gene            complement(4417..5292)
                     /gene="prmA"
                     /locus_tag="PLX80_03065"
     CDS             complement(4417..5292)
                     /gene="prmA"
                     /locus_tag="PLX80_03065"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQY19808.1"
                     /translation="MAEKYYEVKISFNDSNYEDIMNLLYLEGIENILEEEGQLIIYLP
                     EVDKEKSNENSKVRIALLRKSLEITGLIGKKDFSSKLTDDKNWNEEWERSIEPVYIDD
                     KIIVYPSWKEKELNETKGKILIQIDPKMSFGTGHNETTQLVLELMSKYIKGTEENLFD
                     FGCGTGILSIAGIKLGVGKAVAVDIDKDSISNAWEYAEINEVHENIDFIHGSLKDLKY
                     ETFDIVCANIIRSVIVDNFPALTERINPGGKLFLSGILMDDDQEILEHLTQLEYEVVD
                     IQMKTDWIGVFAVKI"
     gene            complement(5285..6169)
                     /gene="folD"
                     /locus_tag="PLX80_03070"
     CDS             complement(5285..6169)
                     /gene="folD"
                     /locus_tag="PLX80_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HQY19809.1"
                     /translation="MELNKLIDGKNISAQIRDEIKTETEILKKEKGIIPGLAFIIAGE
                     DPASKVYVKNKGKACEEAGFHSVTEVLPEGITEIQLLELIWKYNTDENIHGILTQLPL
                     PLHIDPQRVIEAIDPKKDVDGFHPVNVGKLSIGEKCFIPCTPYGIIEMLRRENIETSG
                     KHAVILGRSNIVGKPVASLLLGKDMNSTVTVCHSRTTDIKKITSQADILIAAMGRADF
                     VKKDFIKDGAVIIDVGINRVEDENKKSGYRITGDVDFEDCIEKCSKITPVPGGVGPMT
                     IAMLLRNTLDSAKGEIYG"
     gene            complement(6343..7461)
                     /gene="carA"
                     /locus_tag="PLX80_03075"
     CDS             complement(6343..7461)
                     /gene="carA"
                     /locus_tag="PLX80_03075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQY19810.1"
                     /translation="MTISLSQNQKKHSPKGKLILENGIVFEGKIFGYDGQASGEVVFN
                     TSLTGYQEILTDPSYSGQIVIMTYPLIGNYGVNETDVESEKIHAAGFVVGNYEDEWSN
                     FKGIESLDEYLKKNKVTGIFGIDTRALTKMIRSEGAMRGYITGENLSDKKLLEKVLAS
                     QSMNGLDLVKKVTTPKAYILKSKKPKYRIAIYDYGIKKNILREFLKFDTELKIFNAKA
                     KPEELLDFKPHGIFLSNGPGDPGAVTYAIENIKELISSKVPVFGICLGHQLIAHALGG
                     KTYKLKFGHRGGNHPVKNIEKNSIEITSQNHGFAVDENSLDIDIVKITHTNLYDNTNE
                     GLKHRKLPVMSVQYHPEASPGPNDSKYLFEEFMKIIQK"
     gene            complement(7474..9312)
                     /locus_tag="PLX80_03080"
     CDS             complement(7474..9312)
                     /locus_tag="PLX80_03080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQY19811.1"
                     /translation="MTLNKLSKLSKVHKYSIEKFFSIRTISGFTVSEDDKSIFYIANT
                     SGSPQIWSVPLEGGWADQITTWNDAVKGVYQNPKSHELIFTSDLKGNENLQLYKMDEE
                     NYDVSHLTKDFADGQCFFHSFNKKGTKFLFSTNKRLKYNFDVYIQNYKSGENFLIKSF
                     DDHYPTHAESWSSNERYITYLKFYGNINTDILLHDTKNDTIINITEHDIEEKIFNGGT
                     EFDKKNNGFYYISDEGREFKGIKYYDIKKKKSKWVIKENYDVTGFEFSSDQNLLAWNV
                     NINGSNTPKLKNMKTGKVQKLKLPKGNYGHLTFTHDNKKIVFSCDSPLHPGDIFLYDL
                     KKNTWKQITNSFVGGVSKKGLTKAKDIFYKSFDDVKIHGLFYIPKGLKKDGTNPAIVW
                     PHGGPEHQEMHNFSKYVQIFTNNGYIVICPNFRGSTGYGKSFQKMIYKDWGGAEFKDV
                     LQSVEYLKKTGYADPGKIAMVGGSFGGFMCLTCITKAPDIWKCAIDIFGPSDLFTFVN
                     SVPEHWKKGTDELVGNPERDKELLKERSPINFVENIKCPLLVVQGKHDPRVVEAESEQ
                     IVNKLKEMKKPVEYILLEDEGHGFTKVSNQIIVFKAMINFLDKNLK"
     gene            10046..11599
                     /locus_tag="PLX80_03085"
     CDS             10046..11599
                     /locus_tag="PLX80_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HQY19812.1"
                     /translation="MKKNIYLCIHGHFYQPPRENAWTNEIEPQPSAAPFHDWNERIYQ
                     ECYKPNTEAIIVDSHDNVVKRVNNFEYFSFNFGPTLLSWIRHKHPKTYEKIIEADRIS
                     ADKYDGHGNAIAMVYSHLIMPLANRRDKITQIKWGVADFIFNFGREPEGIWLAETACN
                     EETLEVLCDEGIKFVILDPSQAEKVRSLDKDKNNGKWEDVSSGNIDPRNPFVYYPEKN
                     KKKFINIFFYDGPLSKNIAFDDHIFSSEKLLNRLRQVPVDHKNGDKLISVGVDGETFG
                     HHKHYAERSLSYLFSDLIPESEFEITNFGKYLADHEPENEVKIKKGYRGEGTSWSCLH
                     GVGRWKENCGCGRTDDFPSQEWRKVLRESLDWLRDELSALFEKTGSDYFTNVWKARNE
                     YIEILLDPSKAMLEKFYFNNAKRVLTEKEVTTSLELLEMQKFAMLMYTSCGWFFTDIS
                     GIETLQILEYTKRAMELSHRITGIDPEPEFLKKLSKAKSNLDKYSDGKDLYEKEIIQK
                     RFAHTQNDF"
     gene            11731..13497
                     /locus_tag="PLX80_03090"
                     /pseudo
     CDS             11731..13497
                     /locus_tag="PLX80_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            13607..14107
                     /locus_tag="PLX80_03095"
     CDS             13607..14107
                     /locus_tag="PLX80_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY19813.1"
                     /translation="MKHTSDHWRKSLELRPHPEGGYFKEVHRSEHSVKKDSLPPSFSG
                     DRNISTSIYYMLEENDTSKFHRILSDELWHFYDGDTLLIYEFENDGKMKIHRLGLDTE
                     SGDLPQAVIKAGNWFGAKLLNPETYCLAGCTVSPGFDFNDFEMADRSELIKRYPDHFE
                     IINLLT"
     gene            14325..14759
                     /locus_tag="PLX80_03100"
     CDS             14325..14759
                     /locus_tag="PLX80_03100"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQY19814.1"
                     /translation="MNIFMKEAILEARKGLSEGGIPIGSVLVKDNKIIGRGHNKRVQE
                     SDPVIHAEIDCLRNAGRIGVYRDTVLYSTLMPCYLCAGASVQFGIKKIIAGEDETFNG
                     AREFMESHGIEVINLDLKECKDLMKEFIEKNPKLWFEDIGEL"
     gene            15076..16479
                     /gene="cysS"
                     /locus_tag="PLX80_03105"
     CDS             15076..16479
                     /gene="cysS"
                     /locus_tag="PLX80_03105"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQY19815.1"
                     /translation="MIKLYNTLHRFKEEFISIKPGKVAMYSCGPTVYNYQHIGNFRTF
                     FFEDILYRVLLYNGYDIKYVMNITDVGHLVSDQDEGDDKMEKSAKELGKSVWELSKYY
                     TDVFFKDRELLNILPPDIYTKATDYIEEQINMVKCLDEKGFTYKTSDGIYFDTSLLKD
                     YGKLAKLDIEGLKEGARIEFSNEKKNITDFALWKFSPADEKRQMEWDSPWGTGFPGWH
                     IECSAMSKAVLGTHFDIHCGGIDHIPIHHTNEIAQSEACNGEKFVNYWLHGEFLDLAN
                     EKMSKSTGKFITLQTLIDKGYSPMDYRYFLLTAHYKRKQKFTFEALDSARNGYNHLKS
                     KVINFKSAAEEVRDATSQERADYTKSFLEKINDDLNMPEAMAVLWEVVKDNSLNSGEK
                     LELIYDLDKVFGLELEKAEEEKADVIPDEIRVLANARSEAKSNKDFKKADELRELVKQ
                     KGYDIADRKDGFEIKKI"
     gene            complement(16511..17089)
                     /locus_tag="PLX80_03110"
     CDS             complement(16511..17089)
                     /locus_tag="PLX80_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19816.1"
                     /translation="MKNLLVILILFISIVNAGAQIRWEQISYFHSSGPVSPEYQYNYN
                     IKINSDGTGVLEYTFKGFTTNYDFNVSRSGLNNLNNALTKSQVLDVSMADLKSDKNLI
                     GGSVYNATITMPYSEPTEEYIERQETKKKLGKEEEEELDENGVKTKGKKPIVLIPNNV
                     NEKYSEGIHNLYLAIEKLVPNSAWSKATDMAE"
     gene            complement(17111..17605)
                     /locus_tag="PLX80_03115"
     CDS             complement(17111..17605)
                     /locus_tag="PLX80_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19817.1"
                     /translation="MIKIFFTLFIVLLSSNLYSQDTHWTNLAFYYSNGTVSPDYQYSY
                     QILLNENGEGMVNYKNSEGNKERNFNVSSEDLENFNEYFKGSDVFKVDPDKMRSLDPK
                     DGGSVRTLEVTLYQSPELDAMPPIIRIPEYLNKKYTEDMKCVYDRIESLVPEEIYTEL
                     KIKN"
     gene            18029..19210
                     /gene="nhaA"
                     /locus_tag="PLX80_03120"
     CDS             18029..19210
                     /gene="nhaA"
                     /locus_tag="PLX80_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQY19818.1"
                     /translation="MKITTFRNLGITKLFKEFYESEKTAGILLIICTAISMILANSAF
                     GPEYLNFWQYKIGFTTDAVKLYYPVYYWINDGLMAIFFLLIGLEIEREVYIGELSNFN
                     VALFPIIAAAGGIIVPSLIHLSFNFGTDTQSGMAIPMATDIAFALGILSLLGDKIPFR
                     IKVFLTALAIVDDLGAIIIIAVFYSKGFSLFYFGMAVVVFLILLLMNRKKVNLLYLYL
                     IPGVFMWYFMLKSGVHATIAGVVLAFVIPFGKGDKDSISYKLQHGLHKPVAFLILPLF
                     ALANTGIVFENDWYSGLLSMNSLGIMAGLMLGKPIGIFLFSFAAVKTGIVKSTDKIVW
                     KNIFGVGVLAGIGFTMSIFITLLAFERDSTIINSKIAILMSSLLAGTIGFFLLKMFNK
                     K"
     gene            19599..20738
                     /gene="dnaN"
                     /locus_tag="PLX80_03125"
     CDS             19599..20738
                     /gene="dnaN"
                     /locus_tag="PLX80_03125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQY19819.1"
                     /translation="MKFSVTGEKLVEYLTKVNSVVPTRTPLPILDNILFQLDGDILQM
                     ISSDLEIFMKTELEVKGAGNGSVAVPAKRLLDITRTLTNEKLEIDVNDKNRVTIKSKR
                     GKFTLGGEPADEFPMPEEKEDMNRIEIKGPTVKRFLSKIIHAVNTDEMRRNMAGVLIE
                     ASTDQMKIVATDGFRLGKIIKTNYEHSPDAEEKIVIPYKTAQHILRLNNNEDTLIEYD
                     DHDLKISFDNVMIYSKLIDDTFPNYENVIPADNDKILRIKKQDFSDSLVRALIFADVI
                     TKRVRLELTSTTLQIKADNPEIGSEGEEMIDCSFNSKTGTEDFDKTPFVIAFNAGYLL
                     DAVSQMETEELNVSFGNPSKAAIITPTEQKNDEDFIELIMPVRVG"
     gene            21177..22361
                     /gene="recF"
                     /locus_tag="PLX80_03130"
     CDS             21177..22361
                     /gene="recF"
                     /locus_tag="PLX80_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HQY19820.1"
                     /translation="MILKRIVLKNFRNYSGQEINFNNKFNFIYGNNGQGKTNILEAVS
                     YSTYGKSFLGSSEADCVKFGETEFFVESDFINDLGNKDFIVINYDSHKKSKTIHRNKQ
                     KVNSFSAEVFGRFPLVFLSPKSLNITYGNPSDRRKFFDILISQTSNLYLDHLKELAKT
                     LKQKSALLREYSLHRKLTFSELKDLLNSYNEKLSDVSSRVLFKRINFLREFESYFDRN
                     FQFLLKQDHKGSITYSSDITGEIIPKNSETGLDELRSLTDKILLQKFNEEIKRAVTLV
                     GPQRDEYVFKLSKLENEEPVSFDLKNFASQGEHKTFLLALKLSEYDFLKNSKSTSPVL
                     LLDDILSELDEERVSMIISHLKDYGQIFLTTTDKDYLERLKTFYTDDEISEFYIEDGN
                     VK"
     gene            22366..22677
                     /locus_tag="PLX80_03135"
     CDS             22366..22677
                     /locus_tag="PLX80_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQY19821.1"
                     /translation="MKKINTNDRFNKTVCLKTEMDDFMEYIGLDAKLHELKILNLWDE
                     CVGESISRFSNPVELKKNKLFVKAENAAWRYELSLKKVEIINKLNERLGKKLIREIVF
                     I"
     gene            22704..24713
                     /gene="gyrB"
                     /locus_tag="PLX80_03140"
     CDS             22704..24713
                     /gene="gyrB"
                     /locus_tag="PLX80_03140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQY19822.1"
                     /translation="MAKTKEGSEEVPGYGAGDIQVLKGLEHVRKRPAMYIGDISSRGL
                     HHLVYEVIDNAIDEALAGHCDKITLTLNKDGSVTVTDNGRGIPTDIHPTEKISALEVV
                     MTQLNAGGKFNRDSYKVSGGLHGVGVSVVNALSEWLEAEVVRDGKVYYQKYERGLPKA
                     QVKSTGNAKKTGTKITFYPDHEIFKNINFKFETLEERLRELAYLNKDLIIIIKDERED
                     GSGKTEEFHFKGGLVDFVKYLDETEKSISGKPIFVSGEKENVLVEVAFQYNDSYNSNI
                     YTFVNNINTHEGGTHLEGFKAALTRTLNNFGTKNGLIKSDKITMTGDDFREGLTCVIS
                     IKVPEPQFEGQTKTKLGNSEVKGIVQSITGEQLGNFLEENPSISRKILEKCLRAAEAR
                     EAARKARELTRRKNALEISGLPGKLADCTIKDPAQCELYLVEGDSAGGTAKQGRDRRF
                     QAILPLRGKILNVEKARINRILENTEIRSMVTAIGAGLGNSDEFDESKIRYDKIIIMC
                     DADIDGSHIRTLLLTFFYRHMKEIVDTERLYIAQPPLYKIKKGKTELYAYDENEREEI
                     LKSFKADKNTPAVSDDEDGEADEKINSATSVKGVSISRFKGLGEMNAEQLWSTTMNPE
                     TRTIVLVTNENAAAADKIFRVLMGEEVEPRRKFIEENARYANLDV"
     gene            25024..25701
                     /locus_tag="PLX80_03145"
     CDS             25024..25701
                     /locus_tag="PLX80_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19823.1"
                     /translation="MFSGNEIFAANDSAMVPFSIQNKKSYFTSFSFGMGIIYSNNSSL
                     QDYIEFDVADYNSIPTDERISTFTSGISFFGSAERQIFTDFSLKAEYSYLIKTTDVPY
                     NPNYQYSYYTHQPMLTVNYLLPQKFSYIKFGFGGGYFFSDFTRRYLNNESDYKSSGPA
                     VKFEIVFNAQLGKNAATYFSGFMQKTFMSDLKNSNGNFLNGRDESKVDISSFGMGVKL
                     GLELYFF"
     gene            25724..26554
                     /locus_tag="PLX80_03150"
     CDS             25724..26554
                     /locus_tag="PLX80_03150"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQY19824.1"
                     /translation="MEQIFKAVILGIIQGATEFIPVSSTAHLRVIPALLGWKDIGAAY
                     SAVIQLGTLIATLIYFRKDISQITKGFLNAVKKRDFLTNSDSKIFILIVTGTIPIAVI
                     GLLFKGFIEGDARGLYVISCALILLAIVLYIAEKVGKRDKDMEDITLKDGIIIGFAQA
                     LALIPGSSRSGVTITAGLFRSLKREAAARYSFLLSIPAIGLSGLYELFKERHELLNEN
                     LLSLTIATVVSGITGFLAIAFLINYLKKHSNMIFVIYRIALGILILILLSKGVLTNLE
                     "
     gene            26680..27177
                     /locus_tag="PLX80_03155"
     CDS             26680..27177
                     /locus_tag="PLX80_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQY19825.1"
                     /translation="MKSAKLFIITVFILSSISVAHSQNSENFDSGLKNAKSSGKMILL
                     DVYSDSDNWSKKMDSEVLSTDKVKSALGRVVFIRLNIDQAGNYNYNGKSYSSSELAKL
                     FGGTGYPSFSFMTSDGEIIKFNYNGDEVKNISGYLSEDDFVEMINFFTAGKYKDTDLS
                     TIFTN"
     gene            complement(27186..27674)
                     /gene="coaD"
                     /locus_tag="PLX80_03160"
     CDS             complement(27186..27674)
                     /gene="coaD"
                     /locus_tag="PLX80_03160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQY19826.1"
                     /translation="MKKITAVYPGTFDPVTNGHLDIIERASDLFNEVIVSIAVNQNKK
                     PLFSTDERKDMLVKVTGNIKNVKVDSFEGLLVSYADKLKANVMIRGLRAVSDFEYEFQ
                     MSLTNKKLNPEINTVFLMPNEKYTYLNSSLVRELASYDANVKEFIPDYVLQMLRKKFG
                     YK"
BASE COUNT         9160 a         4753 c         5022 g         8893 t
ORIGIN      
        1 ggaatatttt gatgttcttg cctggataga aagtaaaatg acaccaaaga atttttctga
       61 gatcattaaa tcaaattcaa agataaaact atctgactta aagatataaa aaatacatta
      121 accaatccac atcagcactc ctccgacttc tatgttttaa atctttttga ttttgtccgt
      181 gacggtttta aatttaattt tgtacaggtt ttagaaaaaa ccggtattaa ttaatttaat
      241 aatcgttaag gagtaaatca tgaaaagact ttcaagagca ataatcatct ttgtaacagc
      301 actgggaatc ggtattgttt cggtatcaaa aaattccctg aaagcagatc aatcagaaac
      361 tcagatactg aacccgttca atctattatt tccgcctaca ggactgacat ttgtaactga
      421 accgggcaac aatacacaag ttgacattac atggagcagt gccggagctg ctgctacata
      481 taaatggaaa tttggtgcac caacaattaa ttctgttatt cttagtttat cggcaaacaa
      541 caatggaact tccaccgcac tgacagttac tcctgcagca gttgacggaa ttcttgccgg
      601 acttggatta aatccaggtg attctgtatc aggagaatgg gcagtttggg cttatgaagg
      661 aaatgattca ttaaaatctg ttcagacctt taatattaca ttcaagagaa aagtagtatt
      721 aaaccagttc aatcttgttt cacctccatc aggaattaca cttgtaacat cacctgaaaa
      781 taatgctgag gtaaatataa tctggaaaaa atcttctgca ggcgcaacat ataaatggaa
      841 attcggtgct ccgaatataa attcagtatt gctggagata ccgtcaaaca atgccggatc
      901 agacacaatg cttacagtaa cgcctgcggc agttgacggt atattggcag gattagggtt
      961 gaatcccgga gattctgtgt caggacaatg ggcagtatat gcttataacg aaaccgattc
     1021 tctgaagtct gtagaaacac ataacattac attcagaaga agtaaacttg catcttttgg
     1081 tctgctgttt ccacctacag gaatcacttt tgtatctgag caaggaaata atacacctgt
     1141 aaatattaac tggaacaaat ctgcagatgg agttacatat aaatggaaat tcggatctcc
     1201 taatgtagct ctcaatacaa ttctggatct tgcatcaaat aataatggca tggatacagt
     1261 attgaccgtt acacctgcag cagttgacgg aattcttgca ggacttggtt tgaatcctgg
     1321 tgattcagtt cccggacagt gggcagtttg ggcctatctg aatggggatt cattaaaatc
     1381 agcgcagaca tttgatatta ctttcagaag agataataag aactcaattt ctcagatagg
     1441 ttcagttatt ccagacatat attctttatc acaaaattat cccaatccat tcaatccgtc
     1501 tacaaagatt aattttggaa tacctgagac tggacaggta aatatatctg ttttcgatat
     1561 gtcaggaagg gaagtatcag tggtcctgaa cgaaaataaa tctcccggta actatgaaat
     1621 ttcttttaac ggggcaaatc tgtccagcgg catatattac tacagaatat ctgtccttgc
     1681 aaacggaaag agcttcattg atacaaagaa aatgacactt ataaaatagt tttacttcaa
     1741 acaaccttaa agaaataaaa tttttgttta aaacaaaaaa ccggcagcca tgcattatgc
     1801 tgccggtttt tttattttta aaaaaaatct gtttagtaaa tgttaaaata attttccata
     1861 aaaaattttt ccgtaaaata tttatcctac cagtccggat ttgttttatc aattatcaat
     1921 ccctctaatg cgccaagtgt ataaaaacaa tttcttttgt caaagcttaa aatctgagta
     1981 tctgcactct taagaagttt attaataata tttacataaa atgaatcaat ggaaatatag
     2041 tcgctgagct ctttaaaact ttcagtaggt gaatattcat ccttcaatat ctttgtaaac
     2101 ttaatttctg tgtaccttgc aattcctttc tgccatatct gaaaagaaaa atatctgtag
     2161 tcttttacat ttaacagtga tttaaatttt tctatttctc caatatattt tttcagatta
     2221 acattaaaaa taactgaatc atcagacaga tatgtattca ttgccgactg aatcaacttt
     2281 gaatactgat ctgagatact ttgatcatca taaggaaaat cataattcag catccacata
     2341 cctgtttcat cgccacctga aagatcaaga gaatttacag attcgtaata atccggcagc
     2401 gatgactgca tctgatgaaa atgttcatga agtaaagtga ctgtccagtc aagagaggag
     2461 cggcttgtgt tttcaggctg tccgacaacg atcgttgaaa caccattaac tgccggaaac
     2521 gttgccagca tattattact gaatattctt tctcttacaa atatttcgca gcccgttata
     2581 gagtcaaatc ctatagataa aaaatcctct gaaggtgacg ggtgataaaa taaatattca
     2641 ttttcattta taacaagtaa taaagcaaac agagtttgat taaaattact ccagacatta
     2701 tcaccaaatt catcagacat cctgacagcc tcagcaattc tgaccttatc tgttaatcta
     2761 agttcagaag aatctgtctg cgaataaata ttttttgaac ctaaaattaa agtaagtgta
     2821 atcagaaata agttcaattt taagaatgag tttatgccca tttctaaaaa gtttaattta
     2881 atttttataa ataaaaaacc cttttttaaa ttcagtaaag attttaaaaa agggttaaga
     2941 tctcttaaac ttgtttattt tatgcttcgg caatgtcaag gactcttgga ctttgcgaaa
     3001 caatcagaac gctgttaata atccggactt tctttctctg aaatctctgt aacactttta
     3061 tcttttacat ggtctgggac agtaccgagg acataatccc aaagcgggtt actgactcca
     3121 taagcagtat gatcatcacc gtaatgatgt ttcagatgat aaactttcag aaacttcccg
     3181 atctttcctg tcattttaaa atggtgtgta gcataatgaa tcgaatcata agcaacatat
     3241 ccgagtacaa atcccgcaaa aataattaaa ttatattctc cgaatgcagc ctgaaataaa
     3301 aagaaaaata ctgttgccag aggtaaactc acaagcagcg gcattaccag tcttgttgcg
     3361 tctctcggat aatcatgatg aataccatgt acaagaaagt gaattctttt tccgggttcg
     3421 gattttggat gatagtgaaa tgcccatcgg tgaattacat attccatcag cgtccacaaa
     3481 agaattccga ctacaaaaaa gataattgaa gacattaatg aaattacctg agatccgaaa
     3541 tatgctgttg ccaatataac aggaataaat acaaacaacg gagtcgcagg atgtatgtgt
     3601 gagaaatatt ctaaaaaggg atttttaaat aaccttatag tttcattttt gtttgatacg
     3661 aatttagcca ttacaattta tttatttatg atgaacaaat taagcaattc aaatgtgtat
     3721 aaaaacacaa atttgaatac aaattgtata caatttgatt gtgtttattg ctttcctgac
     3781 ttcagcagat cacgaatctc cataagaaga tgttcttctt tagtaggttc cgcaggagct
     3841 tcatcttcct ctttttttct catgctgtta attcctttta caataagaaa taatacaact
     3901 gcaattatta gaaaattgat agtcgcctga atgaattttc cataaggaaa tactgcacta
     3961 cctatagcga ccgaaagccc gctgaaatca atacctccca taataatacc tataaacggc
     4021 ataaggagat ctgcgacaat tgaacttaca atagcaccga atgcgccgcc gataataaca
     4081 cctatcgcca gatcaattgc atttcccttt gaaataaatt ctttaaattc tttcaacata
     4141 ttgatttttt tttattttat aaaaatatta taatactatt ttaggtttgc ccccattact
     4201 cgaaggctca gagataaaat ttaatgtttt taaattttga gtcttaattc aattccaact
     4261 tttttttcac agttaccaaa gatcaagtga atctgcggat actgaaaaat tattcctcaa
     4321 aaaaatttta acctgtgtaa tttctgtaaa tatattaaca ccaaaattct ttgagtctta
     4381 gagactttga ggtaaacaga tttaatataa aacatgtcag atcttaacag caaaaacacc
     4441 tatccagtct gtcttcatct gaatatcaac tacttcatat tccaactgag tcagatgctc
     4501 gagtatttcc tgatcatcat ccatcaaaat tcccgagaga aaaagtttac cgcccggatt
     4561 aatcctttcc gtcagtgccg ggaaattatc tacaatcaca cttcttataa tgttagcgca
     4621 gactatgtca aatgtctcat attttaaatc tttgagactt ccgtgaataa aatcaatatt
     4681 ctcatgaact tcgttgatct ctgcatattc ccaggcattt gagatcgagt ctttatcaat
     4741 atcaacagca actgcttttc ccaccccaag cttaattccg gctatggaaa gtattcccgt
     4801 accgcatcca aagtcaaaaa gattttcttc tgtgcctttg atatattttg acatgagctc
     4861 tagtacaagc tgagtcgttt cattatgacc ggttccgaaa gacattttcg gatcgatctg
     4921 aatcaggatc tttcctttag tctcatttaa ttctttttct ttccacgagg gatatactat
     4981 tattttgtca tcaatgtaaa caggttcaat tgatctctcc cattcctcat tccagttttt
     5041 atcgtcagtg agtttagatg aaaaatcttt ttttccaatc agcccagtga tctcaagtga
     5101 ttttctcagc agggcaattc ttacttttga attttcatta gacttttcct tatcaacttc
     5161 aggcagataa attattaact gtccttcctc ttcgagaata ttttcaattc cttcgagata
     5221 aagcagattc attatatctt cataattcga atcattaaat gaaatcttta cttcataata
     5281 tttctcagcc ataaatttct cccttcgccg agtctaaagt atttctcaaa agcattgcaa
     5341 ttgtcatagg accgactcct ccgggaacag gcgtgatctt actgcacttt tcaatgcagt
     5401 cttcgaaatc cacatctccc gtaattctgt atcctgattt tttattttca tcctctactc
     5461 tgttaatgcc gacatctata atcactgctc catcttttat aaaatccttt ttcacaaaat
     5521 ctgctctgcc cattgcagcg atgagaatgt ctgcctgtga agtaatcttt ttaatatctg
     5581 tcgtcctgct gtgacatact gtgacggttg agttcatgtc tttaccaagc agcagcgatg
     5641 cgacaggttt tccgacaata ttacttcgtc ctaaaatgac agcgtgctta cctgacgttt
     5701 ctatattttc tctgcgaagc atttctataa ttccgtacgg cgtacagggg ataaaacatt
     5761 tctctccaat ggataactta ccgacattca caggatgaaa tccatctaca tctttttttg
     5821 ggtcgatggc ttctataact ctctgaggat caatatgcaa aggcagaggc agctgtgtaa
     5881 gtataccatg aatattttca tctgtattgt atttccagat cagttcaagt aattgtatct
     5941 ccgtaattcc ttcaggcaga acttctgtca ccgaatggaa acctgcttct tcacaggctt
     6001 tacctttatt tttaacataa acttttgatg cgggatcttc acctgcgatt ataaatgcca
     6061 gacccggaat aatacctttt tcttttttca gaatttccgt ttcggttttt atctcgtctc
     6121 tgatctgagc cgaaatattt tttccgtcaa ttaatttatt caattccatt ctttgttatt
     6181 attaatactt tacaaattat ttctgtacga tcttcataaa cccttcggat tttgtttcct
     6241 cagagtcatg tgctcttcgg actctgtggg tatgaattaa aattaatttt attttattta
     6301 aaaaaatttt ttaatcgtga ttgataaaag agattattta tattacttct ggatgatctt
     6361 cataaactct tcaaataaat atttactgtc attcggaccg ggtgatgctt caggatgata
     6421 ctgcactgac atcaccggca gcttcctgtg cttaagtcct tcgttcgtat tatcataaag
     6481 atttgtatga gtgatcttta ctatatctat atcaagtgaa ttttcatcga ctgcaaatcc
     6541 gtgattctgt gaagtgatct cgatactgtt tttttcaata ttttttacag gatgatttcc
     6601 gcctctgtgt ccgaatttaa gtttatatgt cttgccgcca agtgcatgtg cgataagctg
     6661 atgtcctaga caaattccaa atacaggtac tttagaagaa atcaattctt ttatattttc
     6721 aattgcatag gtcacagcac ccggatcacc cggaccattt gaaagaaaaa ttccgtgcgg
     6781 tttgaaatca agtaattcct caggctttgc tttagcatta aaaattttca gttcagtatc
     6841 aaatttcaga aattcacgaa gtatattttt tttaattcca taatcataaa tggcaattct
     6901 gtactttggc ttttttgatt ttaaaatata tgcctttggc gtagttactt ttttcacaag
     6961 gtcaagaccg ttcatagact gactggcaag gactttctcc aaaagttttt tgtcactcag
     7021 attttcacct gttatataac cgcgcatcgc accttcactt ctgatcatct ttgtcagagc
     7081 gcgtgtatca attccaaaaa ttcctgtcac tttatttttt tttaggtatt catcgagtga
     7141 ttcgatccct ttaaagttac tccactcatc ttcataattc ccaacgacaa atcctgctgc
     7201 atgtatcttt tctgattcga catcggtttc attgactccg taatttccga tcagtggata
     7261 tgtcatgatc actatctgtc ccgaatatga agggtcagtg agtatttcct gatagccggt
     7321 tagagaagta ttgaaaacaa cttcacctga agcctgtccg tcatatccga aaattttgcc
     7381 ttcaaatact ataccgtttt caagaattaa ttttcctttg ggagaatgtt tcttttgatt
     7441 ctgcgataat gaaattgtca attaatgtat tatttatttt aaatttttat cgagaaagtt
     7501 tatcatagct ttgaatacaa ttatctgatt tgagactttg gtaaatccat gtccctcatc
     7561 ttcaaggaga atatactcaa ctggtttttt catttcttta agtttattta cgatctgctc
     7621 agactcagct tccactactc ttggatcatg tttaccctgt acgacaagca acggacattt
     7681 tatattttcg acaaaattta ttggtgatct ttctttgaga agttccttat ctctttcagg
     7741 gttaccgaca agttcgtcag ttcctttttt ccagtgctca ggtacggaat tcacaaaagt
     7801 aaaaagatca gaaggaccga agatatcgat agcacatttc cagatatcag gtgctttggt
     7861 gatacaggtc agacacataa atcctccgaa acttccgccg accattgcaa tcttacccgg
     7921 atcagcataa cctgtcttct taagatattc cacactttgg aggacgtctt tgaattccgc
     7981 accgccccag tctttgtaga tcattttttg aaatgatttt ccgtagccgg tactgcctct
     8041 gaaattcgga cagataacaa tgtatccgtt atttgtaaat atctgaacgt acttgctgaa
     8101 gttgtgcatc tcctgatgct cgggtccgcc gtgaggccac actatcgctg gattggtgcc
     8161 gtcttttttc agacctttcg gaatgtaaaa aagaccgtga attttcacat catcaaaact
     8221 tttataaaaa atatcctttg cctttgtaag accttttttg gaaacacctc ctacgaatga
     8281 attagtgatc tgcttccaag tattcttttt cagatcatat aagaatatat ctcccggatg
     8341 cagaggactg tcgcagctga atacgatctt tttattatca tgtgtaaatg taagatgacc
     8401 gtaatttcct ttcggcagtt ttaatttctg aactttaccg gtcttcatgt tttttagctt
     8461 aggagtattg ctaccgttaa tatttacatt ccatgcaaga agattctggt cagaggaaaa
     8521 ttcaaaaccg gtaacatcat aattttcctt tatgacccat ttactttttt tctttttaat
     8581 gtcataatat tttattcctt tgaattccct tccttcatct gaaatataat aaaacccgtt
     8641 gttctttttg tcaaactccg tacctccgtt gaatattttt tcttcaatgt catgctctgt
     8701 aatatttatg atcgtgtcat tttttgtatc atgaaggagg atgtcggtgt ttatgtttcc
     8761 ataaaatttc agataagtaa tatatctttc attactgctc cagctttcag catgggtggg
     8821 ataatgatcg tcaaaagatt ttatgagaaa attttcacct gatttataat tctgaatata
     8881 tacatcaaaa ttatatttca gtctcttatt tgtggagaaa agaaatttag ttcctttttt
     8941 attaaaagaa tgaaaaaagc actgaccgtc tgcaaaatct tttgtaagat gcgacacatc
     9001 ataattttcc tcatccattt tataaagctg cagattctca ttacctttaa ggtcactggt
     9061 gaagatcagt tcatgagatt ttggattctg ataaacgcct ttcaccgcat cgttccaggt
     9121 agtgatctga tcagcccatc cgccttcgag cggcactgac catatctgcg gactgccgga
     9181 agtatttgca atataaaaaa tactcttatc atcttctgaa actgtaaaac cgcttattgt
     9241 ccttatagaa aaaaattttt ctatggaata cttatgtact ttacttaatt tactcaattt
     9301 atttaatgtc aattaatttt attttaggta tgtgaaagat aactttttta aaaaacaatt
     9361 tttagtctaa acttgggtta ggattggcac aatttcaaat ttacaagttc aggattcaat
     9421 tgttattcta cttttcataa ttctatattg gaaagtgagg tttttcttaa ttcaacgaat
     9481 gtacatacaa tgaatcagaa cttagaagtt tcttgtttca taaagtcatt tgcttttaat
     9541 aatctacgga ttagtagttt taatgttaaa agtatctctt gtcgtatact ttgcaaaatc
     9601 tataaattaa aaaatagtat tgtttcttaa aaacatctcg catacaattg cattactcca
     9661 aaggaattca aatttaaaaa agaaaagtga atcatttaat atttatttac cctctcattt
     9721 attttatacc ttatgaataa ttttattata aaactatttt aataaacgat aattttaatt
     9781 tgtgtaatat cgcattcttc atattcgcag cagcaagagt gggtaagact attcaattca
     9841 ggatcaaatc agaattacaa ggtttcagat ttgaaaattg taaatgaggc attttaagcg
     9901 gagtagcatt gtcactgtct aaactttttc gcagagcatg tgaacttatt ttgccaaacg
     9961 actttaccct tcccaattga taataatttt ttactaacat ttctgaaata ctatttttag
    10021 tttagctgaa aataaaaact tcaaattgaa aaaaaacatt tacctctgca ttcacggaca
    10081 tttttatcag ccgccgagag agaatgcctg gactaacgag atcgagccgc agccttctgc
    10141 agcgccgttt catgactgga atgaaaggat atatcaggaa tgttataagc cgaatactga
    10201 agccattata gtggattctc atgacaatgt cgtaaaacgt gtaaataatt ttgaatactt
    10261 tagttttaat tttggaccga cacttttgag ctggattaga cataagcatc caaagacata
    10321 tgaaaaaata atcgaagcag acaggatcag cgctgataaa tacgacggtc acggaaatgc
    10381 gattgctatg gtgtacagtc atctgatcat gcctctggcg aacagacgcg ataagatcac
    10441 tcagataaaa tggggcgtgg cggatttcat atttaatttt ggaagagaac ccgaaggtat
    10501 atggcttgcc gagactgcat gtaatgaaga gactcttgaa gtgctgtgtg atgagggtat
    10561 taaatttgta attctcgatc cttcgcaggc agagaaagtt aggagtcttg ataaagataa
    10621 aaataatgga aaatgggaag acgtatcatc aggaaatatc gatccgcgga atccgtttgt
    10681 ttattatccg gaaaaaaaca aaaagaagtt tataaatata tttttctatg acggtccgct
    10741 ttcaaaaaac attgcattcg atgatcacat attcagttcc gaaaaacttc tgaacagact
    10801 cagacaggtt ccagtcgatc ataaaaacgg agataaactc atcagcgtcg gagtcgatgg
    10861 agagacattc ggacatcata agcattatgc agaaagaagt ttgtcttatt tattttctga
    10921 tctcattccc gaatctgaat tcgagatcac aaactttgga aagtatcttg cagatcatga
    10981 acctgagaat gaagtaaaaa taaaaaaagg ttatcgtggc gaaggcactt catggagctg
    11041 tcttcacgga gtcggcagat ggaaggaaaa ttgcggctgc ggaaggactg atgattttcc
    11101 ttcacaagaa tggagaaagg ttttaaggga aagtcttgac tggctcagag atgaactttc
    11161 agcacttttt gaaaaaaccg gaagtgatta tttcacgaat gtatggaaag caagaaatga
    11221 atacattgaa attttactcg atccgtcgaa agcaatgctg gaaaagtttt attttaataa
    11281 tgcgaagaga gttctgacgg aaaaggaagt gacgaccagt cttgagctgc ttgagatgca
    11341 gaagtttgca atgctgatgt acacaagctg cggatggttc tttacagata tatcaggtat
    11401 tgagacgctg cagatacttg aatatacaaa acgcgcaatg gagctgtctc acaggatcac
    11461 aggcattgat ccagagcctg agtttttgaa aaagctgtcc aaagcaaaaa gtaatttgga
    11521 taaatattct gacggtaaag atctttatga aaaagagata attcagaaaa gatttgctca
    11581 tacacaaaat gatttttgat tcgttgtaaa taaagaaaac cattttcaaa tattataaat
    11641 aatttggata ctgacctgtt caaatattcg aaaacttctg ccaaatggaa taagatcgga
    11701 ctgcagagac gtgccggtgt tgctcttcct ttgttttcag tttattcttc caaaagtaca
    11761 ggaataggtg agatcgctga catccgttat cttatcgact ggtgcagtgt tacaggactt
    11821 tcgatacttc agttgcttcc tctgaatgag acgggtggtg attttgcgcc ctacagctct
    11881 gtcagcacat ttgccataga cccggtttat atatgtcttc ggagacttcg aaaaacagat
    11941 cttactccat tcagaaaaga gcttcgttag atcaaaaaaa aatttccaaa aaaaaataca
    12001 aaagtcgatt ataagatcag acatgcaaag gtcagtctgc ttcgaaagat ctttgattct
    12061 aaatatactt tcgaatcaga aaattttgaa aaatttattt ctgaaaatat tcactggctg
    12121 aaatattatg cgctgtttaa aataatttca aacctgtatc ctttcaaaca atggactgaa
    12181 tgggatctga aacacagata tctttcatcc tcaaacgccg aaaagatatt aaaaaataat
    12241 cctgaagatg taaggtttca atactgggtt cagtggcagt tatatgagca gcttattact
    12301 gttaaaaaat atgcagcgaa aaaaaaagtt ttcataatgg gtgacatacc ttttctcgtt
    12361 tcgagaaaca gtgcagatgt ctgggcttac aaaaattatt tcaaactcga tctgtcatcc
    12421 ggtgcgccgc ctgatatgta ttttgcaaat ggtcagaggt ggggaatgcc gccttataac
    12481 tggtttaata tagagtcaga taattatgca tacatcagac agcgacttaa gtatgctcag
    12541 aatttttatg acatgttcag gatagaccat tttgtcggtc tcttcagagt gtggactata
    12601 ggaagtgata cacctgcaga tgaaggtgga ctgtcaggaa gattcgatcc ggaaaatgaa
    12661 tcggaatggg aacagcacgg aaggtcaatt ttaaatgtga tgaatgaatg tacagaaatg
    12721 cttccctgtg cagaagatct tggaactgta cctgactgtt ctgaaaaagt tttaaaggag
    12781 tttggcattg ttggaataaa tgtacagcgg tgggaaaaga attataatta taatgacaat
    12841 tttaatttta tatctccgga taagtacagg gtaaattcag tgtcagtatt atccactcat
    12901 gattccagtg catttgctcc ttggtataaa tatgaaaccg gttcattgga tgaaaatact
    12961 tttataaaat tttttgaggg aaaagaattt tcagtcgaaa ggatctaccg gatcagaatg
    13021 aatttatttg aacctaatct gaacaatgat caaagactcg tctggaaaaa aaatatttca
    13081 ggtgtatttg agctgctaaa gatcattgaa ttaaattatg aagaagccag ggaatttgtc
    13141 gatacatatc ttatttcttt ttcagaaaaa aataaataca gcagatacat tggatatgac
    13201 ggtaagcggg aaatgcctgt gaatactgaa tttataaaca aatcactaca gacaattttc
    13261 atttgcaatt ctatcttcag tattcagctt atgcaggaat atcttaatct tgataaggat
    13321 tttttaaaaa attacagcgg tcctgaattc aggataaaca gtccgggcac tgtaaatgat
    13381 cagaactgga caatgagaat tccaatccca ttagataaga tcttaacatt aggtataaat
    13441 tcttccttaa aaaagattgt aaagaattca ggaagatatt ttgatgataa gttttagtat
    13501 ttcagtatcc tcagagtccg aagagcacat gacactgcgg gatctatgaa aatgacttcg
    13561 ggaaaatatt ccgaagaatg atttcaaaca aaaatttttt aaacatatga aacatacatc
    13621 agatcactgg agaaaaagtc ttgaacttcg gccgcatcct gaggggggat atttcaaaga
    13681 agttcacagg tcagaacatt ccgtaaaaaa agattcactt cctccgtcat tcagcggcga
    13741 cagaaatatt tcgacttcca tttattatat gctcgaggaa aatgacactt cgaaatttca
    13801 caggatactt tctgatgagc tgtggcattt ttatgacggt gatactctcc ttatctatga
    13861 atttgagaat gatggaaaaa tgaaaattca cagacttgga ttagatactg aaagcggtga
    13921 tcttccacag gcagttataa aagcgggaaa ctggttcgga gctaaacttc tgaatcctga
    13981 aacatactgc cttgcaggat gtactgtttc tccaggattt gattttaatg attttgaaat
    14041 ggcggatagg agtgaactaa taaaacgata tcctgatcat tttgaaataa taaatctgct
    14101 gacataaatt gttttaagtt tctcaagcag cacaagattt tctgagaatg ttagaactgc
    14161 cattaaaact caaagactat aagtttaaaa gcattaaaaa taaaaacctt tgagccttcg
    14221 ggactttgag gcagggttaa atttactgtt ttcgggcagt atgggaacga gcgaaatagt
    14281 aaatttactg acgtaatttt ttcacaaaca ataaaaacaa tatcatgaac attttcatga
    14341 aagaagcaat actcgaagcg cggaagggtc tttctgaagg cggcattcca ataggttcag
    14401 tgcttgtaaa agacaataaa ataatcggca gaggtcacaa taagagagta caggaaagtg
    14461 atcctgtcat tcatgccgag atcgactgtc tcaggaatgc aggcaggata ggtgtttacc
    14521 gagacacagt attatattct actttaatgc cgtgttatct ttgtgcaggt gcaagtgttc
    14581 agtttggaat aaaaaagatc atagcgggtg aagatgagac tttcaatggc gcacgtgagt
    14641 ttatggaatc tcacggtatc gaagtcatca atctagatct taaagaatgt aaagacctga
    14701 tgaaggagtt cattgaaaaa aatccaaagc tttggtttga agatatcgga gaactgtgag
    14761 aatacattgg acttgccccg aagacacaaa ggctcgaagg tttttaagtt aatcaattga
    14821 ataaaacaga tttttgattt agagtttgga agtaaaatta tgttatgttg aattccagta
    14881 taatatggtt tatcaaagca gggttttgta ttagtttaaa atcacaacct aaaggttgag
    14941 gttactactt ttatttatat agattggtat acaggatctt aaataaattc cattgagtct
    15001 tagagacttt gaggcatgaa ggcaaacttt atctatttca aatttcctta ataaatttta
    15061 gtttaaaaaa attttatgat caaattatat aatacacttc acagattcaa agaggaattt
    15121 atttccatta agcccggcaa agtcgctatg tacagttgcg gaccaactgt atataattat
    15181 cagcatattg ggaatttcag aacatttttt ttcgaagata tactgtacag agtactgctt
    15241 tataacgggt atgatataaa atatgtgatg aatataacag acgttggtca tctcgtttct
    15301 gatcaggatg aaggtgatga taaaatggaa aagtctgcaa aggagcttgg gaaaagtgta
    15361 tgggagcttt cgaaatatta cacggatgtt tttttcaaag accgtgaact tcttaatatt
    15421 ctcccgccgg atatttatac aaaagcaact gactacatag aagagcagat caatatggta
    15481 aaatgtcttg atgaaaaagg attcacttat aaaacttccg atggaattta ctttgacact
    15541 tcgcttttaa aagattatgg taagcttgcg aagcttgata tagaaggtct taaagaaggt
    15601 gcgcgaattg aattttccaa tgagaaaaaa aatatcacag attttgcttt atggaaattt
    15661 tcaccggctg atgaaaagcg tcagatggaa tgggattctc cgtggggtac cggatttccg
    15721 ggctggcata ttgaatgttc ggcaatgagc aaagctgtgc ttggaacaca ttttgatatt
    15781 cactgcggcg gtattgacca tattcccatt catcatacca atgagatcgc gcagtccgaa
    15841 gcatgcaacg gagagaagtt tgtaaactac tggctgcacg gggaatttct agatttagca
    15901 aatgaaaaga tgtcaaagtc cacaggaaaa ttcattactc tgcaaacgct gatagacaaa
    15961 ggatattcac cgatggacta cagatacttt ttgctgacgg cacattataa aagaaaacag
    16021 aagtttactt ttgaagcact cgactctgca aggaatggat acaaccattt aaagagtaaa
    16081 gtaataaatt ttaaatctgc tgctgaagaa gtcagagatg caacttcaca ggagagggct
    16141 gattatacaa aaagttttct tgagaaaata aatgatgatc tcaatatgcc tgaagcaatg
    16201 gctgttctat gggaagtagt aaaagataat tcacttaact ctggagaaaa gcttgagctg
    16261 atatatgatc tggataaagt attcggtctg gagcttgaaa aagctgagga agaaaaggct
    16321 gatgttattc ctgatgaaat aagagtactt gcaaatgcaa gatctgaagc caagtcaaac
    16381 aaagatttta aaaaagctga tgaactgcgt gaacttgtaa aacagaaagg ttacgatatt
    16441 gctgaccgta aagacggctt tgaaataaaa aagatctgaa ttgtaatgat acgatttttt
    16501 aaagcaggaa ttattctgcc atgtcagttg ccttagacca tgctgagtta ggcactaatt
    16561 tttctatcgc aagatataag ttatgtatac cttcagaata tttttcattt acattattcg
    16621 gaattaaaac aataggtttc tttcctttag tttttacacc attttcatcc aactcttctt
    16681 cctcttcttt accaagtttc ttttttgttt cctgtctttc tatatattct tcagttggtt
    16741 cagaataagg cattgtaata gtggcgttgt acactgatcc gccaatgagg tttttatcgg
    16801 atttaagatc tgccatgctg acatccagca cctgactttt tgtaagtgca ttatttagat
    16861 tatttaatcc ggatctgctg acattaaaat cataatttgt tgtaaatcct ttaaaagtat
    16921 attccagtac acccgtaccg tcagaattta tttttatgtt ataattatac tggtactctg
    16981 gtgaaacggg accacttgaa tgaaagtatg aaatttgttc ccagcggatc tgagctcctg
    17041 catttacaat tgatataaaa agtattaata taactaataa atttttcatg ataagattat
    17101 tttataattc ttaatttttg attttcaatt ccgtataaat ttcttcagga acaagtgatt
    17161 cgatcctgtc ataaacgcat ttcatatctt ctgtatattt tttattcaga tattcaggga
    17221 ttctaataat aggcggcatt gcatcgagtt caggagattg atatagagtc acttccaatg
    17281 ttctgactga tccgccgtct ttggggtcaa gagatctcat cttatccggg tcaactttaa
    17341 acacatcact tcccttaaaa tattcgttaa aattttccag atcctcactg cttacattaa
    17401 aattcctttc tttatttcct tcagaatttt tatagttcac catcccttca ccgttttcat
    17461 taagcaatat ctgatagctg tactgataat ccggtgaaac tgtgccgttg gaataataaa
    17521 aagcaagatt agtccagtgt gtgtcctgtg aatataaatt tgatgaaagt aaaacaatga
    17581 agagtgtaaa aaatattttt atcatttttt aaattttatt aatataaaaa ttagagttaa
    17641 aaattataat gttaattttt tatcattact gtaaatcttt ttttcaattc tgatactgct
    17701 aactgcctta agtttaaatt gtttcggaat tttactcaca ctctttctga aaactgtgga
    17761 tttgcctcga aggctctaag actcaaagtt taatgtcatt aaaattataa tgttctgaga
    17821 tttagaattt agtggcaagc taaatatctt ttcccgcaaa gtcttaagac ctggtaagtt
    17881 atatgttaag aattcttatt tgaaatttgt aattcagaaa gttcttcttt gagtctttgt
    17941 gactttgaga taaagcgaca ttgtaaaaag tcaatttaaa tttttaagaa ttgaaactaa
    18001 cagctaattt tttaaaattg acactacttt gaaaattact acatttagaa atctcgggat
    18061 caccaagctc ttcaaagagt tttacgaaag tgaaaagact gccggaatac tactgattat
    18121 ttgcacggca atttccatga tccttgcaaa ttcagctttc ggacctgagt atttaaactt
    18181 ctggcaatat aagatcggct ttactacaga cgcagttaag ctatactatc ccgtttatta
    18241 ctggataaat gacgggctga tggcaatatt ttttcttctg atcggactgg agatcgaacg
    18301 tgaagtttac atcggtgagt tatcaaattt caacgttgcc ctttttccta tcatagccgc
    18361 tgcaggcggg atcatcgttc cttccctgat acacctgtca tttaatttcg gtacagatac
    18421 acagagcggt atggcaattc cgatggcaac tgacattgcc tttgcgctcg gcatactttc
    18481 actcctcggt gacaagatcc ctttcagaat aaaggtcttt cttacagcgc ttgctatcgt
    18541 agatgatctc ggagcgatta ttatcatagc agtattttat tccaaaggat tctcgttatt
    18601 ttatttcggt atggcagtag ttgtttttct aatactgctt ttgatgaaca ggaaaaaggt
    18661 gaatctgctt tatctctatc ttatacccgg agttttcatg tggtacttca tgctgaaatc
    18721 cggtgtgcat gcgacaatcg ccggtgtcgt tcttgcattt gtaattcctt tcggcaaagg
    18781 tgataaagat tctatttcat acaagcttca gcacggactt cacaaaccgg ttgcatttct
    18841 gatactgccg ctgtttgctc tcgcaaatac gggcattgtt tttgaaaatg actggtactc
    18901 gggactgctg tctatgaaca gtctcggcat aatggcggga ctaatgctcg gaaagccgat
    18961 cggaattttt ctgttcagct ttgctgcggt gaagacaggc atagtaaagt ccactgacaa
    19021 aattgtatgg aaaaatattt ttggtgtggg agtgcttgcg ggaatcggat ttacgatgtc
    19081 gatctttatt acgctgcttg catttgaaag agattccact ataataaatt caaagatcgc
    19141 aattttaatg tcatcactcc ttgcagggac aataggtttt ttccttttga agatgtttaa
    19201 taaaaagtag tgttgataag ataaccccat ctttcctaaa cttttagctg caaaattcaa
    19261 gattcatttt ttcctgctta ctttttttaa cgacaaatat aattaacaaa aaaatcgccc
    19321 gcggtgataa cttgccggaa attctattta agttaacaaa taaaattaaa ccgggggtta
    19381 gttcaaactc agagcaatat cacgatcttc tttaatgaat aaattcaggt caaagttctg
    19441 cgatgtctcc gcattaattc tgagaatctg ccggcagtaa aaattaaaaa tccatcctaa
    19501 tttcaatggg aattccgcca aatacactat aatcattgga ttgaaactac ctcattttat
    19561 atgtattttt accaactaaa aataataatc ccagcataat gaaattttct gtcaccggtg
    19621 aaaaacttgt agaatatctt acaaaagtca actctgtagt tccaacccgg acaccacttc
    19681 caatcttaga taatattttg tttcagctcg acggcgacat tcttcagatg atatcatccg
    19741 atctggagat ctttatgaag accgaacttg aagtcaaagg cgccggtaac ggtagcgttg
    19801 cagtacctgc gaaaagactt ttagatatca caaggactct gactaatgag aaacttgaga
    19861 tcgatgtaaa cgataaaaac cgtgttacta taaaatccaa aagaggtaaa ttcacactcg
    19921 gaggcgaacc cgcagacgaa ttcccgatgc ctgaagaaaa agaagatatg aacaggatcg
    19981 agatcaaagg tcctacggta aaaagatttt tgtcaaagat aattcatgca gtcaataccg
    20041 atgagatgcg tcgtaacatg gcaggagtgc tgatcgaagc ttccacagac cagatgaaaa
    20101 tagtcgccac tgacggattc agactcggca agatcattaa gacaaattac gaacacagtc
    20161 cagatgccga agaaaaaatt gtcattccct acaaaactgc acagcacatt cttagactaa
    20221 ataataatga agatacactg atagaatatg acgatcatga tctgaagatc tcttttgata
    20281 atgtgatgat ctattccaag ctgatcgatg atacatttcc gaattatgaa aatgtaatac
    20341 ctgcggacaa tgataaaatt ttacggataa aaaaacagga cttttcggat tcccttgtaa
    20401 gggctttgat atttgctgac gtgatcacga aaagggtcag acttgaactt acatcaacta
    20461 cgctgcagat aaaagcggac aatcctgaga tcggttcgga aggcgaagag atgatagact
    20521 gcagctttaa cagcaagacg ggcacggaag attttgacaa gacaccattt gtaattgctt
    20581 tcaatgcggg atatttactc gacgcagtat cacagatgga aaccgaagaa ctgaatgtca
    20641 gttttggaaa tccatccaaa gctgctataa taactccgac cgaacagaag aatgatgagg
    20701 attttattga gcttattatg ccggtgagag ttggatagaa aacaattaag aattaggaat
    20761 taggaattaa gaattgaaaa ttgagaattt tcttgtagta atttgtaatt tactttaata
    20821 gctaatctat tacatctata ttaatcgcaa tatggtaaga aaatattcag tgaatcttct
    20881 tgagataaat atcaaaattt ttaacagcac gttttagaat aaatcataag taattcctcc
    20941 ttaaaaaagg ggaagcgaag ggggttgttg cagaaagaaa cagcaaaaac tcaacaatga
    21001 aagtagtgag gtaaaattaa actcagctat acaaacccct cccagcctcc cctttgcaca
    21061 aaagggagga gcaataacag tttaataatg ctttttcgtt tctggctata caagcgtatt
    21121 agttagcaga atataattat taactattaa ctactaacta ttaactatat cgggttttga
    21181 tcttaaagcg cattgtatta aaaaatttca gaaattattc cggacaggaa attaatttca
    21241 acaacaagtt caattttatt tacggtaata acggacaggg aaagaccaac atacttgaag
    21301 cagtttcata ttcgacatac ggaaaaagtt ttctcggctc atctgaagct gactgtgtaa
    21361 aattcggcga aactgaattt tttgtcgaat cagattttat taatgacctt ggcaataaag
    21421 attttatagt aataaattat gattcacata aaaagtccaa gaccattcac agaaataaac
    21481 agaaggtaaa ttcattttct gcagaggtct tcggcagatt tccccttgtc tttctttcac
    21541 caaaaagtct gaacattact tacggaaatc cttctgacag aagaaaattc tttgatatac
    21601 tgatctcgca gaccagtaat ctgtatctcg atcatcttaa ggaattagcc aaaactttga
    21661 aacagaaaag tgctttgctg agggaatatt cattacacag aaaacttact ttttctgaac
    21721 tgaaagatct gctgaattct tataatgaaa aactttcgga tgtttcatcg agagtacttt
    21781 ttaaaaggat aaattttctc agggaatttg aatcttattt tgacagaaat tttcagttcc
    21841 ttttgaaaca ggatcacaag ggaagtataa cttacagttc ggatataaca ggtgaaataa
    21901 tcccgaaaaa ttctgagaca ggattggatg agctgagatc gcttacggat aaaattcttt
    21961 tacagaaatt taatgaagag attaaaagag ctgtaacgct tgtcggaccg cagagagatg
    22021 aatatgtatt taagctgagt aaactagaaa atgaagaacc tgtttctttt gatctgaaaa
    22081 actttgcatc acagggagag cataaaactt tcctgctggc gctgaagctt tctgaatatg
    22141 attttctgaa aaacagcaag tcaacgagtc ctgttctttt gctggatgac atactttcag
    22201 agctggatga agaaagggtg tcaatgataa tttcacattt aaaagactac ggtcagatat
    22261 ttttgacaac gacagataaa gattatctgg agagattaaa aacattttat actgatgatg
    22321 aaatttcaga gttttatata gaagatggta atgtaaaata gatttatgaa aaagatcaac
    22381 acaaatgacc ggtttaataa aacagtctgt ctcaagacag agatggacga ctttatggaa
    22441 tacatcggac ttgatgcaaa gttgcacgag ctgaagatat tgaatttatg ggatgagtgt
    22501 gtcggtgaat cgatctcgag attttcgaat ccggtagaac tcaagaagaa caaacttttt
    22561 gtaaaagctg aaaatgctgc ttggagatat gagctttcat taaaaaaagt agaaataata
    22621 aataagctga acgagagact cggcaaaaaa ttaatcagag aaattgtatt tatttaaaaa
    22681 aaattataac cctataagat tatatggcaa aaactaaaga aggatcagaa gaagtaccag
    22741 gctacggagc cggtgatatt caagtattaa aaggacttga gcatgtaagg aaaagacctg
    22801 cgatgtatat cggcgatatt tcgtcgagag gactgcatca tcttgtttat gaagtgatag
    22861 acaatgcgat cgacgaagca cttgcaggac attgtgataa gatcacgctg acattaaata
    22921 aggatggatc agtaacggta acggataacg gtagaggaat tccgactgat attcatccta
    22981 ctgaaaaaat ttcggcactt gaagtcgtaa tgactcagct taatgcaggc ggtaaattta
    23041 acagagacag ttacaaggtt tcaggcggtc tgcacggagt tggagtttca gttgtaaatg
    23101 cactgagtga atggctggaa gctgaagtgg taagagatgg aaaagtttat tatcaaaaat
    23161 atgaaagagg tcttccaaaa gctcaggtga aatcaaccgg taatgcaaag aagacaggca
    23221 cgaagatcac attttatcct gatcatgaaa tatttaaaaa tattaatttt aaatttgaaa
    23281 ctcttgaaga gagattacgg gagcttgctt atctgaataa ggatcttata atcattataa
    23341 aagatgaaag agaagatggt tcaggcaaga ctgaagaatt tcatttcaaa ggcggacttg
    23401 tagattttgt aaaatatctt gatgaaaccg aaaaatcaat ttcaggaaaa ccgatctttg
    23461 tttcaggtga gaaagaaaat gtattggtcg aagttgcatt tcaatataat gattcttaca
    23521 acagcaacat ttatacattt gtaaataaca tcaatactca tgagggcgga actcatcttg
    23581 aaggattcaa ggcagcgctt acgaggacac ttaataactt tggtacaaag aacggtctga
    23641 taaaatctga taaaattact atgacaggcg atgacttcag ggaaggacta acttgtgtga
    23701 tcagcataaa agtacctgag ccgcagtttg aaggacaaac aaaaaccaaa ttaggaaata
    23761 gtgaagtaaa aggaattgtt cagtctatca caggcgagca gcttggaaat ttccttgaag
    23821 agaatccttc aatttcaaga aagatacttg agaaatgtct gagagctgct gaagcaagag
    23881 aagctgcacg aaaagcaaga gagctaacga gaagaaaaaa tgcacttgaa atctcaggtc
    23941 ttccgggaaa acttgcagac tgcactatca aagatccggc gcaatgcgag ctgtaccttg
    24001 tggagggtga ctctgcgggt ggtactgcaa agcagggaag ggacagaaga tttcaggcta
    24061 tacttccttt gagaggaaaa attctgaatg tagagaaagc aagaattaac aggatacttg
    24121 aaaatacaga gatcaggtct atggtcactg caatcggagc gggtcttggg aattcggatg
    24181 aatttgatga gtcaaaaata agatacgata aaataattat tatgtgcgac gccgacattg
    24241 acggttcgca tatcagaaca ttattgctga cattttttta cagacacatg aaagagatag
    24301 tagatacgga gagactttat attgctcaac ctccgcttta taagatcaaa aaaggtaaaa
    24361 cagaattgta tgcttatgat gaaaatgaaa gagaggaaat acttaaatct tttaaggcag
    24421 ataaaaatac tcctgcagta agtgatgatg aggatggtga agcagacgaa aaaataaatt
    24481 cagctacatc tgttaaaggt gtttcaatat caagatttaa aggtcttgga gaaatgaatg
    24541 ctgagcagct ttggtcgact actatgaatc ctgagacgag gactattgta ctggtaacaa
    24601 atgaaaatgc agcagcggca gataaaatat tcagagtact gatgggtgaa gaggtcgagc
    24661 ctagaagaaa gttcatagaa gagaatgcaa ggtatgctaa tcttgatgtg taatggtagt
    24721 caggattttc ttaagttaga tttaattttc acattcggga aaacgttttt aagatctttt
    24781 taaaaagtat aaattaaatt ttaactataa atattttaat ccggtttttt atcaaaaccc
    24841 ctcccaccct tcccctttcc aatgggaggg caagggtgga gtgaaatgaa tgaagaattg
    24901 aatttcaata tttagtgtac attatgttag aatttattca ttaaaaaatt attttagaca
    24961 gcactgtcta ttttcaataa aaattcattt aaatataacc tcttcatatt gctgaacatg
    25021 tttatgtttt caggtaatga gatctttgcc gctaatgatt cggcaatggt acctttcagc
    25081 attcagaaca aaaaaagtta tttcacttct ttttcatttg gcatgggaat catttattcc
    25141 aataattctt cattacagga ttatatagaa tttgacgttg ccgattacaa ctcaattccc
    25201 actgatgaaa gaatttcaac cttcacaagt ggtatttctt tttttggaag cgctgaaaga
    25261 cagatattta ctgacttttc tttgaaggca gaatattcat atttaataaa aaccaccgac
    25321 gtcccttata atccaaacta tcagtattca tattacactc atcagccgat gctgacagta
    25381 aattatttat tgcctcagaa attcagttat attaaattcg gattcggggg tggctatttc
    25441 ttttcggatt ttacaagacg atatttaaat aatgaaagtg attataagtc atccggacct
    25501 gcagtaaagt ttgaaattgt atttaatgct cagcttggaa aaaatgcagc aacgtacttc
    25561 agcggattta tgcagaaaac atttatgtct gatctgaaga attcgaatgg aaattttttg
    25621 aatggacgtg acgaaagtaa agtagatata agcagtttcg gaatgggagt taagctcgga
    25681 ttagaattat atttttttta aattaaaatc ctgaaaaatt ttaatggaac aaatttttaa
    25741 agcagtaata ctcggcatta tacagggtgc aacggaattc attccggtca gcagcactgc
    25801 gcatctcaga gttatacccg cacttctggg ctggaaagat attggcgcag cttacagtgc
    25861 agtgattcag cttggcacac tcatcgccac tctgatatat ttccgcaaag acatttcgca
    25921 gatcacaaaa ggttttttaa atgctgttaa gaaaagagat ttcctcacaa acagtgattc
    25981 aaagattttt attctgatcg taaccggtac cattcctatt gcagtgatcg gacttctttt
    26041 caagggattt atagaaggtg atgccagagg attgtatgtc ataagttgcg ctttgatact
    26101 acttgcaata gtactatata ttgctgagaa agtcggaaaa agagataagg atatggaaga
    26161 cattacttta aaggacggca tcataatcgg cttcgcacag gcactggcgc tgataccggg
    26221 cagttcgagg agcggtgtga cgattactgc gggattgttc agaagtttaa aaagagaagc
    26281 tgctgcaagg tattcatttc tgctgagcat accggctatc ggtctcagcg gattgtatga
    26341 actttttaaa gagagacatg aactgctgaa tgaaaatctg ctgagtctta caattgcaac
    26401 tgttgtatca gggattacgg gatttcttgc gatagctttt ttgattaatt atctgaagaa
    26461 gcacagcaat atgatctttg ttatttacag aattgcactt ggaatactaa tattgatttt
    26521 gctttcaaag ggtgtgctga ctaatcttga gtgattttat tagaaaataa atcaaattaa
    26581 aaaaataatt acttttatat tcagtttgaa attacaagaa acatttagta ttttttacag
    26641 ttaacaaaaa acttgaaatc aaaattaatt taatttagta tgaaatcagc aaagctattc
    26701 ataatcacag tatttatttt aagctcaata tccgttgcac actcacagaa ttcagaaaat
    26761 tttgattcag gattaaagaa tgcaaaatca agcggtaaga tgattctact tgatgtctat
    26821 tcagactctg acaactggag taaaaagatg gattcagaag tactttctac cgataaagta
    26881 aaatctgcac tcggcagagt tgtattcata agacttaata ttgatcaggc aggcaattat
    26941 aactacaatg gtaaatcata ttcttcttct gaacttgcaa aattatttgg aggcacagga
    27001 tatccttcat tttcttttat gacatccgat ggtgaaataa tcaaattcaa ttataacggt
    27061 gatgaagtaa aaaatatttc cgggtactta agtgaggatg attttgtaga aatgataaat
    27121 ttctttactg caggaaaata caaagataca gatctctcaa cgatctttac aaactgatct
    27181 gtatattatt tatatccaaa tttttttctc agcatttgta atacataatc gggtataaac
    27241 tcttttacat tagcatcata agaagccaat tctcttacca atgaagaatt taaataagta
    27301 tatttttcat tcggcattaa aaaaacagta ttgatctcag ggtttaattt tttatttgta
    27361 agcgacatct gaaattcata ttcaaaatcc gacacagcac gcagacctct tatcattaca
    27421 ttcgctttta atttatctgc atagcttaca agcaatcctt cgaatgaatc taccttaaca
    27481 ttttttatat tacctgtaac tttaacaagc atatcctttc tttcatctgt agaaaataaa
    27541 ggttttttat tttgatttac tgcgatggaa actattactt cattaaacag gtcagatgct
    27601 ctttcaataa tatcaagatg tccgtttgta acaggatcga atgtgcccgg atatactgct
    27661 gttatttttt tcattaattt ttattaaaat ccaacaaagt aaaatttact gtgccgatct
    27721 tttttctttt atataaataa ttttcatata tgtcattcaa aacaaaacct gctgaatgtt
    27781 cgagtactaa aaacaatccg cattttgaaa ccccatttat aatatcat
//
LOCUS       DAORVQ010000020        27240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_610_length_27240_cov_6.545669, whole genome shotgun sequence.
ACCESSION   DAORVQ010000020 DAORVQ010000000
VERSION     DAORVQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 27240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27240
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_610_length_27240_cov_6.545669"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..513)
                     /locus_tag="PLX80_03165"
     CDS             complement(70..513)
                     /locus_tag="PLX80_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19827.1"
                     /translation="MKIKIASSENIEALAELFDLYRQFYEQPSDISAAKTFLSERISN
                     NESKIFVCCDDDGNGMGFTQLYPYFTSVGMKRAWILNDLFVKKEFRKMGAAEELINAV
                     RKFASETDAAYVMLETHISNDAACKLYEKTGFIKDEEHFYFYLKV"
     gene            complement(604..2160)
                     /locus_tag="PLX80_03170"
     CDS             complement(604..2160)
                     /locus_tag="PLX80_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY19828.1"
                     /translation="MKKKIEISISPEENADEKKLDEIFRRISRVKKEEEFHVRLLNRS
                     IDARSRYPVINITAEVYINEPPVPEPVFSENYKKVSDKKRVFIAGAGPAGYFAALELL
                     EHGIKPVIFDRGKDVRERRRDLRAIQQEGIVNPDSNYCFGEGGAGTYSDGKLYTRSHK
                     RGDIYKALKILTEHGAHSDILIDSHPHIGSNRLPVLVQKLRETILHYGGEINFNSTVT
                     DLIISDGKINGIIINDSEELFSDNLILATGHSARDIYYMLKRKNILTEAKPFAVGVRV
                     EHPQTLIDEIQYKQNPRSEYLPAAPYKLTAQCGGRGVYSFCMCPGGLIVPSATSPGEL
                     VVNGMSMSRRDSKFANSGIVAAVELEDLKNYKDHGSFMGLEFQKEIERIFFKEGDGGQ
                     KAPAQRLTDFVNGKLSDTLPESSYIPGIYSADLNNILSKEISSKLREAFSQFGKKMKG
                     YLTEEAIVTGPESRTSSPVRIPRDRLTFEHVQMKGLYPCGEGAGYAGGIISAALDGQN
                     TAKAIAMKLL"
     gene            complement(2288..2641)
                     /locus_tag="PLX80_03175"
     CDS             complement(2288..2641)
                     /locus_tag="PLX80_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQY19829.1"
                     /translation="MNIEAFRKFCLNKKGVTEDFPFGEDTLCYRVMEKIFAITSFSLP
                     FSVNLKCDPEKAVEYRERYDEVKPGYHMNKKHWNTVMFDGTIPDKLLKEMTDDSYNLI
                     VKGLNKKQKEELLKL"
     gene            complement(2763..3344)
                     /locus_tag="PLX80_03180"
     CDS             complement(2763..3344)
                     /locus_tag="PLX80_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQY19830.1"
                     /translation="MKSVCVFCGSNSGKGVDYIVSAQKLGKLLAGNGIDLVYGGGNIG
                     IMGELANTVLQYNGKVTGVIPEDLVAREVAFKDATELRIVKTMHERKALMSELSEGFI
                     MMAGGIGTLEEFFEVWTWAQLGIHDKPIGILNTNNYFDKLLDFIYKAVDDKFVHEKYL
                     EMIIVEHEPYMLLDKMSMFEPVKVRKWIGKEDI"
     gene            complement(3362..3868)
                     /locus_tag="PLX80_03185"
     CDS             complement(3362..3868)
                     /locus_tag="PLX80_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQY19831.1"
                     /translation="MPEFKFGLFTSFVFFLWMVLVYTIIVPNFYGAGEFIMFVSVFIP
                     TIGIFLGIKERRDKSNFGHITYREAFRSGIVITFIISVMIVIFTYVYYEFINPDYVNF
                     LIAKSEKSMIADNVPREQINAALTVTRYQFSLNVQIIQQLLFVLIGGSVISAVLAFIL
                     KKEKRSKN"
     gene            complement(3984..4250)
                     /locus_tag="PLX80_03190"
     CDS             complement(3984..4250)
                     /locus_tag="PLX80_03190"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQY19832.1"
                     /translation="MDNAQQEKENHEKLVTVYETGNEAVVAVIKSVLDEAGIRYLAKG
                     EGVQDLFGVGILGTGFNPITGPVQFRVMPEDQEYATELLKDIDG"
     gene            4589..5494
                     /locus_tag="PLX80_03195"
     CDS             4589..5494
                     /locus_tag="PLX80_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY19833.1"
                     /translation="MYNPFKEIKINTDTSKSDVTEDNQLVSDALTGDKKSLELLIKRH
                     QAWIYNIALKMVWDPVDAEDVTQETLIKIITKLSTFRGDSAFRTWAYRIVANHVINMR
                     KRKKEFEYTSFKVYGEQIIRSPDTDLPDKESVPVDVNLLIEETKIGCMNAMLLCLDRE
                     QRLVFILGSMLGVNDSTGSEILDISSDNFRQKLSRARKDMSNFMSNKCGLMDKNNPCH
                     CNKKTKLMIDAGHVNPEKLLFTRQRIHSIEMVSEKRKEDIDDLFEKKATKLFREHPFQ
                     DSPDFVNSVNQILSNKDFKRIFNFN"
     gene            5691..6158
                     /locus_tag="PLX80_03200"
     CDS             5691..6158
                     /locus_tag="PLX80_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY19834.1"
                     /translation="MNNQKLEIKAAIQILKPAAEVFEAIADPEKMSNYFISESSGRME
                     EGKNLNWRFPEFDMDVPVRVGKIEKDKYVSYYWDDPDGTELFVEITLTPKEKNATLVS
                     ITERSRENNEEGIKWLKRNTEGWANFLACLKAYLEYGVNLRKGAFDYMKKEDL"
     gene            complement(6332..7792)
                     /locus_tag="PLX80_03205"
     CDS             complement(6332..7792)
                     /locus_tag="PLX80_03205"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY19835.1"
                     /translation="MKILFSLLLMLISFNVALPQGWFTQNNGSSNTITEIYFVNENTG
                     WLCGSGGMLMNTTNGGTNWTAQTSNTFETLWALYFTNENNGWATGDNGIILTTTNGGT
                     NWIARTSGTFSALWYVHFVNVNTGWISGSNGEILKSTNGGANWTAQTSGTAQILYSVF
                     FINDQTGWVTGNGGVIRKTVNGGTNWTAQTSGTGTLLHCVNFTDGNNGWITGFNGIIL
                     HTTNSGTNWNFQTSGTGVFIRSIDFVNSTTGFAAGDNGTLIATTNAGLNWQSQNSNAV
                     NHFYSLVALDPLTAWATGENGLIRKTITGGNILRQLDLTMLIEGFYNEGNNRMTPDTI
                     SVLIKNSSTPYNTIDSQKAKTDSIGNTSLNFQNAVNGVSYYITLKHRNSLETWSSVSV
                     MFISNDLDYDFTLTDTNAFGNNMTLRGSRWAVYSGDANQDGTVDLADLSLIDNDAANF
                     QTGYITTDINGDGLADISDLGITDNNSFNFVSAVRP"
     gene            7930..8391
                     /locus_tag="PLX80_03210"
     CDS             7930..8391
                     /locus_tag="PLX80_03210"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19836.1"
                     /translation="MTQQITEIFNYEKWANTEIIKAIEKTNSPSEKVLSLMSHIINAQ
                     IVWLSRINNTIPDTEVWQLYKKDELIVRHTVSIDNILNFLNEQKDVDLEKIITYENSK
                     GEKFTTALKDILIHLSHHSGYHRGQIISLLKSEGEEFPYTDYIHYIRNIKK"
     gene            8398..10038
                     /gene="ade"
                     /locus_tag="PLX80_03215"
     CDS             8398..10038
                     /gene="ade"
                     /locus_tag="PLX80_03215"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HQY19837.1"
                     /translation="MSEKNFTVKGNIVDIFERRIFPGSVTVKDGKIFSIEQDSNTYDT
                     FLMPGFVDAHVHVESSMLIPSEFARIAVTHGTVSTVSDPHEIGNVLGVDGIRYMINNG
                     NKVPFKFYFGASSCVPATSFETAGAEITADDIRELFEKDGLHYLSEMMNYPGVLFDDD
                     MVMEKIKIAKEFGKPIDGHAPGLRGMDAEKYISAGISTDHECFTLDEALDKLRYGMKI
                     IIREGSAAKNFEALHPIIKDFPDMVMFCSDDKHPDDLILGMMDRVAARAVDKGYDVFD
                     VIRCATFNPVTHYNLNTGLLRAGDPADFIEVKDLNEFKVIKTYIDGELVAENGKSLIP
                     RIVESVVNNFNTDKKNICDFEIKGDDNQMIRVIEALEGQLITNEIHAPLKSADGNLLP
                     DIENDILKITVVERYLSGKPSVAFIKNFGLKDGAIASCVAHDSHNIIAVGTNDTDICN
                     AVNAIIENKGGVSVSVNGKTDSLALPVAGIMSADDGYEVAGKYSKLDKLAKELGTKLH
                     APFMTLSFMALLVIPQLKLSDKGLFDGKKFEFVDLKII"
     gene            10241..12109
                     /locus_tag="PLX80_03220"
     CDS             10241..12109
                     /locus_tag="PLX80_03220"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HQY19838.1"
                     /translation="MRSILILFFITIFYSSLSAQSNQTTQDNTYKFFIDLNKVVDDKI
                     TIELITPKFTESEISYKFPAMVPGTYKVYNFGNYISDFKAVDESGNNLSVNKGDANTW
                     SISEADKLYKISYRVDDTFDDTIQEVTVFEPVGTNINEGNQFIFNNQGFFGYFDGYLG
                     NEYQLTFYKPEGFYGSSSMTALSRGSSEDSFTSPNYQFLVDNPIMYTKPDTTSINFDE
                     ANIMISVYSESKTISSKDLEKDIKNLIIAIRKHLGGKLPSDKYTFIYNFSSEGTSGSY
                     GALEHNLSSFYHFPDVQKQAVPYVLKQLNSTNAHEYYHTVTPLNLHSEEIGNFDFNNP
                     IMSEHLWLYEGTTEYFADYIQLREGLLDMKEYLSGLEGKINSSMKFNDSLPFTVMSKG
                     ALDIYEDQYLNVYQKGALIGLCLDIVIRESTGGEMGYQDVINKLLEQYGKDRSFKDED
                     LFNDIEAFTNPTVRAFLDTYVSGSERIPYDVYLGKLGLDIKADSYESISMAGGLQMGF
                     NQKNYRLKIVSVDNSKSEFLKDMGVQAGDELLTLNGTPVSFMNIQDAFGSAGNSIKTG
                     DKMEIEVARYDSAGKESKVKLSAVIKDTKTSYDYTISISKTPTDEQKKLMSAWYGK"
     gene            12240..12668
                     /locus_tag="PLX80_03225"
     CDS             12240..12668
                     /locus_tag="PLX80_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19839.1"
                     /translation="MEIIKFTYLKLILISFLILTSFTFYNCSGYTPEFSNNRTKLNSQ
                     LQTMINVLNGAHYSQFIQEYVDPGYISKAGGLDQVVLSFNNEKQRQIFAALKAAQNID
                     PVSNSKGTVMTYSGGALPVAVMFRQVNNKWYLTGDWLEYN"
     gene            12707..13150
                     /locus_tag="PLX80_03230"
     CDS             12707..13150
                     /locus_tag="PLX80_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19840.1"
                     /translation="MKNIKINFFTLVFVPSFILSTIIFSGCGGSSSDTTSSILVFKAE
                     LNNQMQEMQNTLDDANYVMFMQNYVDPGYISDIGGINEAVTLMDISRQTELFVSLQLA
                     KNIEPIYSKSSNVMKYIGGGLTIPMTFVQINNKWFLTRESFLITR"
     gene            complement(13294..14313)
                     /locus_tag="PLX80_03235"
     CDS             complement(13294..14313)
                     /locus_tag="PLX80_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HQY19841.1"
                     /translation="MKNIILFLILTTISLISNKTYSQDTFSICAIDTITGEVGSAGAS
                     CVNDCTILTDVFPGWGIVHTQASWLSANQNYARSLMLLRLDPQAMIDSLVAHDAQNNP
                     SVRQYLIINKGNGTPKRAAYTGANCLNYKNHIIGPNYVIAGNILSGQPILDSMEARFL
                     REEGSISDKIMAALQGAKVVGADTRCASWGTSSKSAFIRVAKPSDTLGILYLNLKVLN
                     TPFGRDPIDSLQVLYNNWTLTGITSAANEVVSGYKLFQNYPNPFNPKTIINYELQITG
                     YDFVNLKVFDVLGNEVATLVNEKQNSGSYTVDFDGSNFPSGMYYYKMEVNGILKDVKR
                     MVLLK"
     gene            14450..18256
                     /locus_tag="PLX80_03240"
     CDS             14450..18256
                     /locus_tag="PLX80_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY19842.1"
                     /translation="MNKTHTDILDPRFGFNFKDLFQSAKLKELSEIFFEYFKNKDVVN
                     YEFFKNYSDKKGLEHSETEISKILIKSSLILSDFLVELFQLQKEKKEFLSEADYENDI
                     FIFKKDFVQKRVLKYYKIEDLVEFSFDDLDKFILAVKESVYPEFNFNIDEEKYTAKMI
                     LELSEIEKNYRWFYEGDKFTPDNFTIPENIRSKAELIMRILYEKGILLFGNIKNIESI
                     ENINRTENSDDGKLMYEKFNYLLKEIEKWIFVKKYLDKNSQHWVSFSDIHKTDYNHLV
                     KSEQPGSDFPELIKVPEKDHRNRFGFRLTDQRMNKREIADEVDYCIYCHERGKDSCSK
                     GYTDRSGEIKTNPLGIKLEGCPLCEKISEMHFVKKSGHPLASLALIMIDNPNVPGTGH
                     RICNDCMKGCIFQTQEPVNIPQIETNVLTEIFKLPYGYEIYSLLSRWNPLNIKQPYAL
                     PYNGKNTMIVGMGPAGYTLAHYLLNDGFGVIGIDGLKIEKIHEKYTGTVNENGYTKFP
                     EPVKDYFTEICEELDKRVLQGFGGVSEYGITVRWDKNFLTAIYINLARREFFRLYDGV
                     RFGGTLTIDDVWDYGIDHIAIATGAGKPTIIRMKNNLIRGIRKASDFLMTLQLTGASK
                     KNNLANLQVQLPAIVIGGGLTAIDTSTELLAYYPVQVLKAYEKYIAIKENSGEEEFWK
                     KLNEEESEIMKTFIEHGKEIRLLIDKAEQDNSIPDTASLTKKWGGVKMVYRKGLEDSP
                     AYRLNHEEIIEALKEGIEIVEHLSPVEAVSNKFGAVKEMVFQFSDKVEDESKRNTLMT
                     LKAKSVMVAAGTSPNIIYEKEYPGTFELDADNYFFKSYKLVTDNRGEKKLIEVETDET
                     GFFTSYEKDGKYITFYGDNHPVYAGNVVKAMASAKDGYEKVSELFENEIKIIKENRET
                     SEMLKNNFQDLVTNLDTDLIATIEKVERLTPNIIEVVVKAPAASRKFKPGQFYRLQNY
                     ETGAENLDGSVLTTEGLAMTGAWTDNEKGLISVIILEMGVSSNLCSRFKKGEKVVLMG
                     PTGTPTEINSGETVLLAGGGLGNAVLFSIAKGLKEKGNKVIYFAGYKNSQDVYKMDDV
                     EAGTDQVIWSIDAGEEIIPRREQDLSFKGNIVEAMNAYSEGVLGNKLFDFGSIDRMIV
                     IGSDRMMKAVKDSRKTIFKDVLKEHVAIGSINSPMQCMMKEICAQCLQRQVDEVTGKE
                     SFIFSCFNQDQDLDNVDFDNLHQRLKTNSVLEKLSDFYLAGLLYKNENVFEKNSGEKD
                     SIMEGH"
     gene            complement(18618..18815)
                     /locus_tag="PLX80_03245"
     CDS             complement(18618..18815)
                     /locus_tag="PLX80_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HQY19843.1"
                     /translation="MIIYKDIEIFCPYCGEVNFMSIELSNNSSDEFITDCEVCCRSFT
                     VNVFSDSNGEISFDVKNDDGF"
     gene            complement(18812..19771)
                     /locus_tag="PLX80_03250"
     CDS             complement(18812..19771)
                     /locus_tag="PLX80_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HQY19844.1"
                     /translation="MDFILLLLGFAFLIFGADKLVDGASSLASKFGIPNIVIGLTVVA
                     FGTSAPELFVNFVAAVNGNTQMVLGNVIGSNIFNVLVILGICSLFYPIAVKRNTTWLE
                     IPLSLLAGLMVLIISNDIFLDKTSSNIISSSDGIILFCFFLIFHVYTMEVAKSETSDE
                     QFETKDYSKPKSVLFIIIGLAGLVIGGELIVTNAVRIASDFGISERIIALTIVSTGTS
                     LPELVTSIVAVRKKNVDIALGNIVGSNIFNIFFILSISAFITPLTVEDSNQTEIYVNI
                     YANILLFLFIFVGKGRKIERWEGIILIISYLLYIIVLIYNIQF"
     gene            complement(19785..20414)
                     /locus_tag="PLX80_03255"
     CDS             complement(19785..20414)
                     /locus_tag="PLX80_03255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY19845.1"
                     /translation="MESLAETPVTITLLFANIAISIWAMYSNPIIMYKLSEWPYQIVH
                     EKKYYQLITSAFLHANFMHLFFNMFALFTFGTYLESFFINNLGALEGSLYFFLIYFIS
                     MLFGSVLSVVFHYNDPQYVAVGASGAVSGIVFSYIIFFPQSMLYVFFIPMPAYIFAFL
                     WLAFSVYGMKKKLGNIGHEAHLGGALGGVISTLILIDGSMRILLSHFNL"
     gene            complement(20427..21458)
                     /locus_tag="PLX80_03260"
     CDS             complement(20427..21458)
                     /locus_tag="PLX80_03260"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQY19846.1"
                     /translation="MIYKIFIRPFLFMMDPESAHNCVLNFFSKVRIFDPLIKILYSPK
                     NNEPIQVSDLAFRNKLGLSAGFDKNGAAIKFWDVLGFSHIEIGTVTPLSQSGNPKPRM
                     FRLKKDSAVINRLGFNNKGADEIRKNILIAKKTLSKDFVIGINIGKNKDTPIENSISD
                     YKICFEKLYDVADYFTLNISSPNTEKLRTLHESENLSELLSQIQLLNNELSQKFSKNI
                     KPVFLKISPDINNINAQSVYENAVTHKLTGIIATNTTVSRSGLSEDINETGGLSGKPV
                     KILSDEILNIFNELNKKNSGYKLILIGCGGIFNSSDVVDKIKKGAALVQIYTGFIYEG
                     PSIIKKILN"
     gene            complement(21586..21659)
                     /locus_tag="PLX80_03265"
     tRNA            complement(21586..21659)
                     /locus_tag="PLX80_03265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21623..21625),aa:Pro,seq:tgg)
     gene            complement(21986..23137)
                     /locus_tag="PLX80_03270"
     CDS             complement(21986..23137)
                     /locus_tag="PLX80_03270"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY19847.1"
                     /translation="MKILNLIKISENPVLGFLTKFQISNYLKSILILMLFCLPVKTMS
                     NIHSTQSFTPVAFPPAGWTTVHVSGLLGSGSWARATSSYLTSPGCAESPGTLLGDNWM
                     MSSQYTPVSGDSLIFYVSSNYLITALGRLEVKVSTTGNVVANFLDFVLPVNITLGLLT
                     PNTYYRRAVSLEDYVGTPIYIAFRHIEVAGLFGDLRLDGISIKNDNFPSTLNLTALVE
                     GHVNFRDRDTVTVNLRSATSPYNVIETAVEYLDTLGKKSIDFFFADDSESYYIVVDHR
                     NSINTWSKGGGELLPAGLTPYDFTTGVNKAYANNMKLVTGKATYLTGDINEDEFVDIT
                     DMAAIDNDSFNFVFGDYVITDLNWEEFVDISDYALCDNNVFQFAIEIQP"
     gene            complement(24130..26820)
                     /locus_tag="PLX80_03275"
     CDS             complement(24130..26820)
                     /locus_tag="PLX80_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19848.1"
                     /translation="MKSKLILLLAIAVFAGLTYLLSLLIDKTKTEPLENINSNKKKST
                     GFLENSDNSDNSDNSDNSKDNYSKEKKVNVSGAMESMQWISQIRAYPEKDIPHDKYYE
                     AFEYSKENLQEVNNNDGNDQWTSLGPNNIGGRAVSIAIHPVDTNVVFLGTASGGLWKS
                     TTGGTGATAWTLVNTGFPSSAIGAIAIDSANPNVMYIGTGENYGYMFSKNGLDVRVTR
                     GMYGIGILKTTDGGITWTKSLDWTYNNERGVWKVLINNKNSNILYAGTSEGIYKSVNA
                     GTTWTQVLDYPMVMDLELNNSDTSVIYASIGNLTNNVPQTNKGIYKSTNSGSTWIKLI
                     GGLPESWTGKTTLELYKGNQNFVYASISNDFSYVGYFKSTNAGLNWTQLSTTIPIGSQ
                     GWYNNGHIVKPDDPNTMLVGTIDVEKSVNGGSSFNTKSDWSAWVEGATPPGEPEGPDN
                     FVHADVHFFASNPKDNNKVYIAADGGLYRSNNFGETFFSCNGGLVTTQFYASFANSYQ
                     DSIFCLGGLQDNRSTFYQGNNAWYKTFFGDGMSCAINSQNDNICYTEYTYGSMYKSTD
                     RGLNWSGISPPGSGNGNNYCFVTPFISCVSNPQIMYVGGKSIYKSTTGGGNWQGPYGT
                     SVFNNEKILSLAVSQTGTDTLYCGLVNGTVFRSFNGGLNWTNISTNIPNRYVTDITIN
                     PNNSSEVYLTLGGYNSGHIYRSVNAGSTWQDISGTLPNVPHQSVVADPVYNQNIYAGN
                     DLGVYVTTNGGVNWNEYSSGMPYTLVFDLTIVYSNRKLRATTHGNGIYERKLMETPVG
                     ISQNNQSQISGYKLSQNFPNPFNPKTVINYELQVTGVELVNLKVFDILGNEVLTLVNE
                     NQNAGSYSVTFDGNNFPSGVYYYRLDAGDFVETRRMALIK"
BASE COUNT         9084 a         4538 c         4717 g         8901 t
ORIGIN      
        1 atattttaat ttactgtgag ttggtataac ggttaaatat gaaaaagcat tagtactttt
       61 taaaataact caaactttta aataaaaata aaaatgctct tcatctttta taaatccagt
      121 cttttcatat aacttgcatg cagcatcatt tgaaatgtgt gtctcgagca ttacatacgc
      181 agcatctgtt tccgatgcaa attttctgac ggcatttatt aattcttcag ctgcccccat
      241 ttttctgaat tcttttttta caaaaagatc attgagtatc cacgcgcgtt tcataccgac
      301 tgaagtgaag taaggataaa gctgtgtgaa ccccattcca tttccatcat catcacagca
      361 tacaaaaatt ttagattcat tatttgaaat tctttcggag agaaaagttt ttgctgcgga
      421 tatgtcagaa ggttgttcat aaaactgcct gtagagatca aaaagttctg caagtgcttc
      481 aatgttctct gaagatgcta ttttaatttt catagttagt ttatattaat gtcaccctta
      541 cggggttttc ataattaatt gtcttcgaag ttcagattat tgaatataaa atgttgtcag
      601 aatttatagt agtttcatag caattgcctt tgccgtattc tgaccatcga gagctgcaga
      661 gattattcct cctgcatatc ctgcaccttc accacatgga taaagtcctt tcatttggac
      721 atgttcaaat gttaaacggt ctcttggaat tctcacgggt gagctcgtcc tagattcagg
      781 accagtgaca attgcttctt ctgttagata tcctttcatt ttttttccaa actgagaaaa
      841 agcttcacgc agtttcgatg agatctcttt agataaaata ttattcagat ctgctgaata
      901 gattcccggt atatatgaac tttccggtaa agtgtcagat aattttccat taacaaaatc
      961 agtaagtctc tgtgcaggag ctttctgacc gccgtcacct tctttgaaaa aaattctttc
     1021 tatctctttt tgaaattcaa gacccatgaa tgaaccatgg tctttataat ttttcagatc
     1081 ttccagttca actgctgcaa caattcccga gtttgcaaat tttgaatcac gtcgggacat
     1141 tgacattcca tttacaacca gttcgcccgg tgaagtagca gagggcacga tcaatccgcc
     1201 cggacacatg cagaatgaat agactcctct gcctccgcat tgtgctgtaa gtttataagg
     1261 agctgccggc agatattcac ttctcggatt ttgtttgtat tgaatttcat cgatcagagt
     1321 ttgcggatgc tcgacacgca ctcccacggc aaagggtttt gcttcagtta aaatattttt
     1381 tctttttaac atataataaa tatctcttgc tgaatgaccg gttgcaagta ttaaattatc
     1441 tgaaaaaagt tcttccgaat catttattat aattccattt atttttccgt cgcttatgat
     1501 cagatcggta acagtcgaat taaaatttat ctcgccgcca taatgcagga tcgtttcgcg
     1561 aagcttctgc acaagtacag gcagtctgtt agaaccgata tgcggatgag agtctatgag
     1621 aatatccgaa tgtgctccat gttcagtaag aattttcagg gctttataaa tatcacctct
     1681 cttatgtgaa cgagtatata acttcccgtc agagtaagtt cccgcaccgc cttcaccgaa
     1741 gcaataattc gaatcaggat ttacaatacc ttcctgctga atagcacgca gatctctgcg
     1801 ccgctcacgg acatcctttc cgcggtcaaa aattaccggc ttaattccgt gctcgagaag
     1861 ttcaagcgct gcaaaatatc ccgcaggtcc cgcaccggct atgaacactc tttttttatc
     1921 agatactttt ttataatttt cactgaagac aggttcggga acaggaggtt cgttaatgta
     1981 aacctcggca gttatattaa taaccggata tctgctgcgt gcatctatag atctgtttaa
     2041 aagcctaacg tgaaactctt cttctttttt gactctgctg atccttcgga atatttcatc
     2101 aagttttttt tcgtcagcat tctcttcagg tgatatcgaa atttcgatct tttttttcat
     2161 gtgtgttcac tatttatgtg aaaaaatttt tgattggttt atattaaact aactcaaagt
     2221 ctgaaattaa tatatctcaa atggaataga caaattgaag ttcattaaat tttgtctgta
     2281 aaaaatatca gagtttcaaa agctcttctt tctgtttttt gttaagtcct tttactataa
     2341 ggttataaga atcatctgtc atttctttta aaagcttatc gggtatagta ccgtcaaaca
     2401 tcactgtatt ccaatgtttc ttattcatat gataacccgg ttttacttcg tcatatcttt
     2461 ctctgtattc aacggctttt tcaggatcgc atttcagatt tactgagaaa ggtaagctga
     2521 atgaagtgat agcaaaaatt ttttccatta ctctatagca aagtgtgtct tcaccaaatg
     2581 gaaaatcctc tgttacgcct tttttattta agcaaaattt tctaaaagcc tctatgttca
     2641 attttcaatt attaatgttc aatgttcaat gctttttgta ataacctctt aagtttgatt
     2701 tatctaaaat tatttagttc actaataaaa taagtgcaac attgtaaatt gtacattgaa
     2761 cattaaatgt cttctttacc tatccatttt cttactttga caggttcgaa catactcatt
     2821 ttatcaagta acatataagg ttcatgttcg acaataatca tttcaagata tttttcgtga
     2881 acaaatttat catcaactgc tttataaata aagtcgagca gcttatcaaa ataattattt
     2941 gtattcaaaa ttccgatcgg cttatcatga ataccaagct gagcccacgt ccagacttca
     3001 aagaattctt ccagtgtccc gattcctcct gccatcatta tgaatccctc tgacaattca
     3061 gacattaaag cttttctctc atgcatagtc tttacaattc gaagttctgt tgcgtcttta
     3121 aatgcaactt cccgtgcgac gagatcttcc ggtataacgc cggttacttt tccattatac
     3181 tgcaggactg tatttgcgag ttcacccatt attccgatat tgcctccgcc atagacaagg
     3241 tcaattccat ttccggctaa aagctttccg agcttttgag ctgagacaat ataatctact
     3301 cctttacctg agttagatcc gcaaaatacg caaacacttt tcataatttt attcagtggt
     3361 tttagttctt acttcttttt tcttttttta aaataaatgc caatactgct gaaatgactg
     3421 aacccccaat aagtacaaat aataactgct gaatgatctg aacattaaga ctgaattgat
     3481 aacgggtaac tgttagagca gcattgatct gttctctcgg tacattgtca gctatcattg
     3541 acttttcgga tttagcaatc agaaaattaa cataatcagg atttataaat tcataataga
     3601 cgtaagtaaa aattacgatc ataactgaaa taataaacgt gattacaatt ccagacctga
     3661 atgcttctct gtatgtaata tgcccgaaat ttgacttatc ccgtctttct tttattccta
     3721 aaaaaattcc aatagtcgga ataaatactg aaacgaacat tataaattca ccggctccgt
     3781 aaaaatttgg aacaattata gtataaacca ataccatcca taaaaaaaat acaaaagaag
     3841 taaatagtcc gaacttgaat tcaggcataa aaatttatta aaataattta tcaatttaaa
     3901 aatattcaga catgatcaaa aaaatatatt gatcattatg agaatattca aaaaatttta
     3961 tatgttgata cttaagaaaa atatcaaccg tctatatctt tgagtaattc agttgcatat
     4021 tcctgatctt ccggcattac tctgaattga acaggtcctg taataggatt aaatcctgtt
     4081 cccagtattc caactccaaa aagatcctgg acgccttcgc cttttgcgag atatctaatt
     4141 ccagcttcgt cgagtacaga ttttatcact gcgaccacag cttcattgcc ggtctcataa
     4201 acagtgacta atttttcatg attttccttt tcttgttgtg cattatccat aatagtttta
     4261 tttagtttaa tattaatgtt tgaaaatatt ttcttttatt caatttaaat ttatttgctt
     4321 attaattcat tttaaaataa tcttttttaa ataataaata tatgtaaagc tataacctga
     4381 caaattacag caaaagtaaa gttgtatatc ccatataaat ggttttcaga agaaatttat
     4441 tggattctaa ttcaaaacat ttaacattga acattttaaa ttataatttg aacattgatc
     4501 attgaaaatt attttgtcac acatcaataa aaaacatgtc taaatattaa aatgaatttt
     4561 attaaattaa ataaaaaaag aaactgacat gtataatccg tttaaagaaa ttaaaataaa
     4621 tacagacaca tcaaagtcag atgtaacaga agacaatcag ttggtcagcg atgcattgac
     4681 aggagataaa aaaagtcttg aacttctgat caaacgtcat caggcatgga tttataacat
     4741 tgctttgaaa atggtctggg atcccgtgga tgccgaggat gttacacagg aaactcttat
     4801 taagatcatt acaaaacttt ctacattcag aggcgatagt gctttcagga cctgggctta
     4861 caggatagtt gcaaatcatg tgataaatat gcggaagagg aaaaaagaat ttgaatatac
     4921 atcattcaaa gtgtatggtg aacagatcat tcgttcgccg gatactgacc ttcctgacaa
     4981 agaaagtgtt ccagtcgatg taaatctttt aattgaggaa acaaagatcg gctgcatgaa
     5041 tgcgatgctg ctttgtcttg accgtgagca gcgtttggtt tttattcttg gaagtatgtt
     5101 gggagtcaat gacagcacgg gttcggagat actggatatt agcagtgata atttcagaca
     5161 aaagctttca cgtgccagaa aagacatgtc aaacttcatg agcaataaat gcggacttat
     5221 ggataaaaat aatccgtgtc attgtaataa aaaaacgaaa ctcatgattg atgcaggtca
     5281 tgtaaatcct gagaaacttt tattcacacg ccaaagaatt cacagtattg aaatggtttc
     5341 agaaaaaagg aaagaagata tagatgatct ttttgaaaaa aaggcaacca aactttttcg
     5401 ggagcatccg tttcaggact cgccggattt tgtcaattca gtgaatcaga ttttgtcaaa
     5461 caaagatttt aaaagaatat ttaattttaa ctgaatcagt tataaaccca gtctataaga
     5521 cccttagttt ctaaatataa aaaaagagtt acacttagct tgaatgtgac ttaagtgatt
     5581 tgtaatttta ttttaaaaga tatctacacc tgaaaatttt aaattaatgg atttaaaaaa
     5641 tatgtcatgc tttcactaat gaaagtattt agaaaattta atcaaaatta atgaacaacc
     5701 aaaaacttga gatcaaagca gcgattcaga tacttaaacc tgccgctgaa gtatttgagg
     5761 caatcgcaga tcctgaaaaa atgtcaaatt attttatttc agaaagcagc ggcagaatgg
     5821 aagaaggaaa aaatctcaac tggcgctttc ctgaatttga tatggacgta cctgtcagag
     5881 ttggcaaaat agaaaaggat aaatatgttt catattactg ggatgatcct gacgggacag
     5941 aattatttgt cgaaattaca cttactccaa aagaaaaaaa tgcaacactt gtttctatta
     6001 cagagcgaag cagggaaaat aatgaggaag gtattaaatg gcttaagaga aatacagaag
     6061 gctgggcgaa ttttctcgca tgtctgaaag catatcttga atatggagtt aatttaagga
     6121 aaggtgcttt tgattatatg aagaaggaag atctttaatc aaaattttaa ataatttgaa
     6181 ttttgaattt tgaatttttt gtaattaaag tttttgtaat ttacaatagt atcattttta
     6241 tagttagaaa aattgatttt gaaaacttca acccaagact tatataacat ttctcacata
     6301 gaacaatgta aattgtacat tgtacattaa tttatggtct aactgcactc acaaaattaa
     6361 acgaattatt atccgtgata ccaagatcag aaatatcagc aagcccgtca ccgtttatat
     6421 cagttgttat ataaccggtt tgaaaattag cagcatcatt atctatcaga ctcaggtctg
     6481 caagatcgac agttccgtcc tgatttgcat cacctgaata aacagcccaa cggctgcctc
     6541 ttaatgtcat attatttcca aaagcatttg tatcagtcaa tgtaaaatca taatcgaggt
     6601 cattggaaat aaacataact gatacagaac tccaggtctc aagtgaattt ctgtgtttaa
     6661 gagtaatata ataacttaca ccgttgacag cattctgaaa attcaaagaa gtatttccaa
     6721 tagaatcagt tttggctttt tgtgaatcga ttgtattata aggagtggat gaatttttta
     6781 tcagaaccga tattgtatcc ggtgtcatac gattattccc ttcattataa aatccttcaa
     6841 tgagcatagt caggtcaagt tgtctcagaa tatttccacc ggtaatagtt ttcctgatta
     6901 aaccgttctc acctgtagcc caggcagtca acggatcaag tgccacaagt gaataaaaat
     6961 gatttacagc attactgttt tgtgactgcc agttcaatcc ggcatttgta gtcgcaatta
     7021 aagtaccatt atcacctgca gcaaaacctg tagttgaatt tacaaaatca attgagcgga
     7081 taaagacacc tgttcccgaa gtctgaaaat tccagttcgt gccgctatta gtcgtatgga
     7141 gtataatgcc attaaaccct gttatccatc cgttattccc gtcagtaaaa tttacacagt
     7201 gaagcagcgt acccgttccg ctggtctgtg cagtccagtt cgtgccgccg tttacagttt
     7261 tcctgatcac gccgccgtta cctgtcaccc agccggtctg atcatttata aagaatactg
     7321 aatataatat ctgtgctgtg ccgctagttt gtgctgtcca gtttgcaccg ccgttagtgg
     7381 atttaagaat ttcaccgttg cttcctgaaa tccaacctgt gtttacattt acaaaatgta
     7441 cataccagag agcagagaac gttccactgg ttcgtgcgat ccagttcgta ccgccgttgg
     7501 tggtggtaag tataattccg ttatctcctg ttgcccatcc gttattttca ttggtaaaat
     7561 atagtgccca cagggtttca aatgtatttg aagtttgcgc tgtccagttc gtaccgccgt
     7621 tagtagtatt cataagcatc cctcctgaac cgcacagcca gccggtgttt tcatttacaa
     7681 aatatatttc ggtgatggtg tttgatgatc cgttgttttg tgtgaaccag ccttgcggca
     7741 atgcaacatt aaaggaaatt aacattaata aaagtgagaa taaaattttc attcggtttt
     7801 ttttttacaa agataatatt ttttgagtta agtttcattt ctttaagtac acagaataat
     7861 attatatgct tcaatttcaa attgaattgt aaaaaattat tgtaataaat agctttacaa
     7921 aaaattttta tgacacaaca gatcactgaa atattcaatt atgaaaaatg ggctaacacg
     7981 gaaattataa aagctattga aaaaacaaac tctccctccg aaaaagtttt aagtctgatg
     8041 tcacatatta taaacgctca gatcgtctgg ctgagcagaa taaacaatac tatacctgac
     8101 acagaggtat ggcagttgta taaaaaagat gagctgattg tcaggcacac ggtttcgata
     8161 gacaacatat tgaattttct gaatgaacag aaggatgttg atcttgaaaa aattattaca
     8221 tacgaaaatt ccaaaggaga aaaattcact acggctttaa aagatatttt aatacatctg
     8281 agtcatcatt ccggatatca cagagggcag atcatttcac ttttaaaatc agagggcgaa
     8341 gaatttccat ataccgatta catacattac atcagaaata ttaaaaaata aatttttatg
     8401 tcagaaaaaa atttcacagt aaaaggaaac atagtagata tattcgagag acggatcttt
     8461 ccgggaagtg tcacagtgaa ggacggtaag atcttttcaa tagaacagga cagtaataca
     8521 tatgatacat ttctgatgcc gggttttgtg gatgctcatg tgcatgtgga gagttcgatg
     8581 cttataccgt ctgaatttgc gaggatagcg gtgactcatg gaaccgtttc aacggtttct
     8641 gacccgcatg agatcggaaa cgtgctcgga gtagacggta tcaggtatat gataaataac
     8701 gggaataaag tgccgttcaa attttatttc ggtgcatctt cctgtgtgcc tgcaacttct
     8761 tttgaaactg ccggtgctga gattactgcc gatgatatca gagagctttt cgagaaagat
     8821 ggtctccact atctcagtga aatgatgaat tatccgggag tactgtttga tgatgatatg
     8881 gtcatggaaa aaattaaaat tgctaaagag tttgggaaac ctatagatgg tcatgctccg
     8941 ggactcagag gcatggatgc agaaaaatat atttcggcag gcatttctac tgatcatgaa
     9001 tgctttactt tggatgaagc tttagacaaa ctcagatacg gtatgaagat aattatccgt
     9061 gaaggttcgg ctgcaaaaaa tttcgaagcc cttcatccga ttataaaaga ttttcctgat
     9121 atggtcatgt tttgcagtga tgacaaacat ccggacgatc tgattcttgg aatgatggac
     9181 agagttgctg caagagctgt cgataaaggg tatgatgtat ttgatgtcat cagatgtgct
     9241 acatttaatc cggtaactca ttataatttg aacacaggat tattgagagc cggcgacccg
     9301 gctgatttta ttgaagtaaa ggatctgaat gaatttaaag taataaagac atatatagac
     9361 ggagaacttg tcgctgaaaa tggaaaaagt cttattccac gtatagtcga atcagttgtg
     9421 aataatttta atactgataa aaaaaatatc tgtgattttg aaattaaagg tgatgacaat
     9481 cagatgatcc gagttattga agcacttgaa ggtcagctta ttacaaatga aattcatgct
     9541 ccacttaaat ctgcagatgg aaatcttttg ccggatattg agaacgatat tttgaaaatc
     9601 actgtagtag agagatatct ttcgggaaaa ccttcagtag cattcattaa aaattttgga
     9661 ttgaaagacg gtgccatagc atcatgtgtc gctcatgatt cacataatat tattgctgtc
     9721 ggaactaatg atactgacat ttgtaatgct gttaatgcga tcatcgaaaa taaaggaggc
     9781 gtttctgttt cagtgaatgg aaagacagat tcactcgcat tgcctgtagc aggtatcatg
     9841 tcggccgacg acggatatga agttgccgga aaatattcga agcttgataa acttgcaaaa
     9901 gaacttggca ctaaactgca tgcgccgttt atgactttgt catttatggc attgctcgtc
     9961 atcccgcagc ttaaacttag cgataaagga ttgttcgatg gaaaaaagtt tgaatttgta
    10021 gatcttaaaa ttatttaact ctgatcattc aaataattat caatattatc aataccatca
    10081 ataccacaat ccggttaatt cattttatgt tataatatct attgaaatca aatcagtacc
    10141 aatattagat atcttctgca tttaatatat cattggcaac aaactttaaa gtcacacgta
    10201 attttcataa cttattaaca cttaatttaa aattatattt atgcgatcaa tattaatact
    10261 gttttttata acaatttttt actcatctct ttccgctcag tctaatcaaa ctactcagga
    10321 caatacctat aaatttttta tagatttgaa taaggtcgta gatgataaga ttacaattga
    10381 gctgatcact ccgaaattca ctgaatctga aatttcttat aaatttcctg caatggtgcc
    10441 cggtacatat aaagtttata attttggaaa ttacatctca gacttcaaag cagtagatga
    10501 atccggaaat aatctttctg ttaataaagg tgatgctaat acctggagta tttccgaagc
    10561 agataaattg tataaaattt cttacagagt agatgacaca tttgatgata caattcagga
    10621 agttactgtc tttgaaccgg tcggaacaaa tatcaacgaa gggaatcaat tcatatttaa
    10681 taatcagggc ttcttcggat attttgacgg atatcttgga aatgaatatc aacttacttt
    10741 ctataagccc gaaggatttt atggatcttc aagtatgact gcgctaagca gaggtagtag
    10801 cgaagattca tttacatctc cgaactatca gtttctcgtt gataatccta taatgtacac
    10861 taagcctgat actacatcta taaattttga tgaagctaat ataatgatct cggtgtattc
    10921 ggaaagtaaa actatttcct caaaagatct ggaaaaagat attaagaatc taattattgc
    10981 gattagaaaa catctcggcg gtaagctgcc ttcagataaa tatactttca tttataattt
    11041 ttcttcagaa ggtacttcag gaagttacgg tgcacttgag cacaaccttt cttcattcta
    11101 tcattttcct gatgttcaaa aacaagccgt tccatatgtc ctcaaacaat tgaattcaac
    11161 taatgctcac gaatattatc atactgtaac tccgcttaat ctacattcag aggaaattgg
    11221 aaactttgat tttaataatc ctataatgag tgagcatctc tggctttatg aaggcaccac
    11281 agaatatttt gccgattata ttcagcttag agaaggattg ttagatatga aagaatattt
    11341 atccggctta gaaggtaaaa ttaacagctc aatgaaattt aatgattctc tgccttttac
    11401 tgtaatgagt aaaggtgcac ttgatattta tgaagatcag tatttaaatg tatatcagaa
    11461 gggtgcgctg attgggcttt gtctggatat tgtaataaga gaatctaccg gcggtgaaat
    11521 gggttatcag gatgtgataa ataaacttct tgaacaatac ggtaaagata gatcttttaa
    11581 agatgaagat ctttttaatg atattgaagc atttactaat ccgactgtaa gagcattttt
    11641 agatacttat gtcagcggct cagaaagaat tccttatgat gtctatctgg gaaaattagg
    11701 tctggatatt aaagcagatt cttatgaatc aatttcaatg gcaggtggtt tgcaaatggg
    11761 tttcaatcag aaaaattaca gattaaaaat tgtaagtgta gataacagca aaagtgaatt
    11821 tctaaaagac atgggtgttc aggctggtga tgaactttta actcttaacg gtacacctgt
    11881 ttcatttatg aatattcagg atgctttcgg atctgcaggc aacagtatta aaaccggtga
    11941 taaaatggaa atagaagttg caagatatga ttcagccgga aaagaaagca aagtaaaatt
    12001 aagcgcagta attaaagata caaaaacttc ttatgactat acaatttcaa tttctaaaac
    12061 acctacagat gaacagaaaa aattaatgtc tgcatggtat ggtaaataaa ttaattcttc
    12121 cataaaatat aaacaccact ttaaattaaa gtggtgtttt ttttatgcat taaaaaatcc
    12181 tgtaacttga atcaaaatat ttttatgttt aattttccat aactaaaaat attttgataa
    12241 tggaaatcat aaaatttact tatttgaagt taattcttat ttcatttcta atattaactt
    12301 catttacttt ttacaactgc agcggatata ctcctgaatt cagtaacaac agaacaaaac
    12361 ttaattcgca gcttcagact atgatcaatg ttctgaatgg tgctcattat tctcagttca
    12421 ttcaggaata tgtagatccc ggttatatct caaaagccgg gggattagat caggttgtgc
    12481 tttcgtttaa caatgaaaag caaagacaga tctttgcagc tttgaaagca gcacaaaata
    12541 tcgatccggt ctcaaacagt aaaggtactg taatgacata ttccggaggg gcattgcctg
    12601 ttgctgttat gttcagacag gtgaataaca aatggtatct gaccggggac tggctggagt
    12661 ataattaaat atttttgtca tatccaaaat ttaaccaact gactctatga aaaacataaa
    12721 aattaatttt ttcactttag tgtttgttcc ttcatttata ttatcaacta ttatcttttc
    12781 aggatgcgga ggatcttctt cagatacaac ttcaagtatt ttagtattca aagctgaact
    12841 gaacaatcag atgcaggaaa tgcaaaatac acttgatgac gcaaattatg taatgttcat
    12901 gcagaattat gttgatcccg gatatatttc agatattggt ggaattaatg aggctgtaac
    12961 tctcatggat atttccagac aaaccgaatt gttcgtttcg cttcagcttg caaaaaacat
    13021 agaaccgatc tacagcaaaa gtagtaatgt tatgaaatat attggcggag ggcttaccat
    13081 tccaatgacc tttgttcaga tcaataataa atggtttctg acgagggaat cttttttaat
    13141 tacaagataa gtttataaag taattttttt tggaaaattt ctcacagagt atctattacc
    13201 ctaaagtata tataaccaaa gggatattac cttttttatc acagcgtaga ttagaagcgg
    13261 gaagtgttta tatttgtaat ttgtaatttg aaattatttt aaaagcacca ttctctttac
    13321 atcttttaga attccattca cttccatttt ataataatac attccgcttg gaaaattact
    13381 tccgtcaaaa tcaaccgtgt aacttcccga attctgtttt tcatttacta atgtcgcaac
    13441 ttcatttccc aaaacatcaa atactttcag atttacaaaa tcgtaacccg taatttgtaa
    13501 ctcgtaattg ataattgtct ttgggttaaa tggatttgga taattttgaa aaagtttata
    13561 acctgatact acttcatttg ctgcagatgt gataccggtc agcgtccagt tattataaag
    13621 cacctgtagt gaatcgatcg gatcccttcc aaacggagta ttcaatactt tcagattaag
    13681 ataaagaatg ccaagagtat cagaaggttt tgcaacgcga atgaatgccg actttgatga
    13741 agttccccag cttgcacatc ttgtatctgc cccgactact tttgctccct gaagtgctgc
    13801 cattatttta tctgagatcg aaccttcttc tctgaggaat ctcgcttcca tcgaatcaag
    13861 tatcggctga cccgaaagta tattaccggc aattacataa tttggaccga taatatgatt
    13921 tttataattc agacaatttg ctcctgtata agctgcacgt tttggagtgc cgttaccttt
    13981 gttgataata agatattgtc tgactgacgg attattctga gcgtcatgag ctacaagcga
    14041 atctatcata gcctgcggat caagtctcaa aagcattaat gaacgtgcat aattctgatt
    14101 tgcagaaagc catgacgcct gagtgtgtac aattccccag ccaggaaata cgtccgtcag
    14161 aatagtacag tcattgacac atgaagctcc tgcgcttccc acttcaccgg ttattgtatc
    14221 gatcgcacag atcgaaaatg tatcctgtga ataagtttta ttgctgatta atgatattgt
    14281 tgtaagaata agaaagagta ttatattttt catatagaat ttttatttgt acaaattaac
    14341 aattaattca ttacagaaaa atgtatcaat gaaagttatt ccaaatctga acttaaagtg
    14401 catttaataa aattctgaaa tgcagtaatt ttgtattcat taaaattcta tgaataaaac
    14461 acatactgac attctcgatc cccgtttcgg atttaatttt aaagatcttt ttcaatctgc
    14521 aaaattaaaa gagctgtcag agattttttt cgaatatttt aagaataagg atgttgtgaa
    14581 ttatgaattt tttaaaaatt attcggataa aaaaggctta gaacattctg aaacagagat
    14641 ctcaaaaatt ttaatcaagt catcacttat attatccgat tttctcgtcg aattattcca
    14701 gttgcaaaaa gagaagaaag aatttttaag tgaagcagat tatgaaaatg atatttttat
    14761 ttttaaaaaa gattttgttc agaaaagggt tcttaaatat tataaaattg aagatcttgt
    14821 agaatttagt tttgatgatc tagataaatt tatattggct gtaaaagaat ctgtatatcc
    14881 tgaatttaat ttcaatattg atgaagagaa atatactgct aagatgatac ttgagctttc
    14941 tgagatcgaa aaaaattaca gatggtttta tgaaggtgat aaattcactc ctgataattt
    15001 taccatacct gaaaatatta gatccaaagc cgaattaata atgagaatac tttatgaaaa
    15061 aggaattctc ttatttggaa acataaagaa tattgaaagt atagaaaata taaacaggac
    15121 agaaaattct gatgatggaa agcttatgta tgaaaaattc aattatttat tgaaggaaat
    15181 tgaaaaatgg atctttgtaa aaaaatatct tgataagaat tctcaacact gggtctcttt
    15241 ttctgatatt cataaaactg attataatca tttggtaaag tcagaacagc ctggctctga
    15301 ttttcctgaa cttatcaaag ttccggaaaa agatcaccga aacagatttg gattcagact
    15361 tactgaccaa cggatgaata aaagagagat cgcagatgag gtagattact gtatttattg
    15421 tcatgagaga ggaaaggatt cctgctctaa aggttacaca gaccgttcgg gagaaattaa
    15481 aacgaatccg ctcggaataa aacttgaagg ctgcccgctt tgtgaaaaaa tttcagaaat
    15541 gcattttgtg aaaaagtcag gacatccttt agcttcactg gcattaataa tgatcgataa
    15601 tccaaacgta cctggaaccg gtcacagaat ctgcaacgac tgtatgaagg gctgtatttt
    15661 tcagactcag gaacctgtga atattcctca gatagaaacc aatgtgttga cagaaatttt
    15721 taaacttcct tatggatatg aaatttattc acttctttca agatggaatc cgctgaatat
    15781 taaacaacct tatgcacttc catacaacgg gaaaaataca atgatagtgg gaatgggacc
    15841 ggctgggtat actcttgccc attatttatt gaatgacggt tttggtgtga tcggaattga
    15901 cggactgaag atcgagaaga tccatgaaaa atatacaggt actgtaaatg aaaatggata
    15961 tacaaaattt cctgagccgg taaaagatta tttcacagag atctgtgaag agcttgacaa
    16021 aagagtgctg cagggatttg gcggagtttc ggaatacgga ataactgtca ggtgggataa
    16081 aaattttcta acggcaattt atatcaatct tgcccgcaga gaatttttta gattgtatga
    16141 cggagttaga tttggtggta cacttacgat agatgatgtc tgggattacg gaatcgatca
    16201 cattgcaatt gcaaccggtg ctggaaagcc gactattatc agaatgaaaa ataatttgat
    16261 cagaggcatt agaaaagctt ctgatttttt gatgacgttg caacttacgg gagcttcaaa
    16321 aaaaaacaat cttgccaatc ttcaggtgca gcttccggca attgtgattg ggggcggact
    16381 gactgcaatt gatacttcta ctgaattact tgcttattat cctgtacagg ttttgaaagc
    16441 ttatgagaaa tatattgcaa taaaagaaaa ttccggtgaa gaagagttct ggaaaaagtt
    16501 aaatgaagaa gaatcagaga tcatgaaaac ttttattgaa catggaaaag aaattcggct
    16561 gttgatagat aaagctgaac aggacaattc aatacctgac acagcttcac ttacaaaaaa
    16621 atggggtggt gtcaaaatgg tttacagaaa aggacttgaa gattctcctg catacagact
    16681 caatcatgaa gaaataattg aagcgctgaa ggagggaatt gaaattgtcg aacatctttc
    16741 acctgtcgaa gcagttagca ataaatttgg tgctgtgaaa gaaatggtgt ttcagtttag
    16801 tgataaagtg gaagatgaga gtaaaagaaa tactctaatg actttgaaag ccaaatcagt
    16861 gatggttgca gcaggaactt ctccgaatat aatttacgaa aaagaatatc cgggaacatt
    16921 tgagcttgat gcagataatt attttttcaa atcttataaa ttagtaacag acaatagagg
    16981 agagaaaaaa ttaatagaag ttgaaacaga tgaaactgga ttttttactt cttatgagaa
    17041 agacggaaaa tatataacat tctatggaga caatcatcct gtatatgcag gcaacgtagt
    17101 aaaagccatg gcttcggcaa aggacggtta tgaaaaagta tcagagcttt ttgaaaatga
    17161 aataaaaatt atcaaagaga atcgcgaaac atcagagatg ttaaaaaaca attttcaaga
    17221 tcttgtaaca aatcttgaca cagatctgat cgcaacgatt gaaaaagtag aaagacttac
    17281 tccgaatata attgaagttg ttgtcaaagc tcctgccgct tcaagaaaat tcaaacccgg
    17341 acaattttac agactacaga attatgaaac aggtgcagaa aatttggacg ggtcagttct
    17401 cacaacagaa gggctggcga tgaccggtgc ctggacagat aatgagaaag gtttgatatc
    17461 agtgattata cttgagatgg gagtttcgtc aaatctctgc agcagattta aaaaaggcga
    17521 gaaagttgtc ctgatgggac cgactggtac accgactgaa ataaattccg gagaaactgt
    17581 tttacttgcg ggtggcggac tcgggaatgc agttttattt tcaattgcaa aaggactgaa
    17641 ggaaaaagga aataaggtaa tttattttgc cggatataaa aattcacagg acgtatataa
    17701 aatggatgat gtggaagcgg gtactgatca ggtaatctgg agtattgacg ctggggaaga
    17761 aattattccg cgaagagaac aggacttgag ttttaaagga aatatagtgg aagcaatgaa
    17821 tgcgtattct gaaggagtat tgggaaataa attatttgac ttcggaagca ttgacagaat
    17881 gatagtgata ggaagtgaca gaatgatgaa ggcagtcaaa gactcacgga agacaatttt
    17941 caaagatgtg ttaaaagagc atgtcgctat cggcagtatt aactcaccta tgcagtgtat
    18001 gatgaaagag atctgtgctc agtgtctgca gagacaggtt gatgaagtta caggaaaaga
    18061 gtcatttata ttttcatgtt ttaatcagga tcaggattta gataatgtag attttgataa
    18121 tttacatcaa cgactgaaga ctaattcggt tctggagaag ttgagtgatt tttatcttgc
    18181 aggattgctt tataaaaatg agaatgtttt tgaaaaaaat tcaggggaga aggatagtat
    18241 tatggaaggg cattaatttt atcccattca ttccgtaatt ttttctgaat tgagagagca
    18301 tccattttaa actttacctt tccacagtat ttagatgctt tgaaaagttt ttcagattta
    18361 aattgtaatg atgctctgat tttcttttca atatcttttt tttctctgat ttttttatta
    18421 caattaccat atttgattta tttaactatt ataaagtaaa tttttaaatc aaacatttaa
    18481 attgaatttt cataaactca ggcggaatag ccggtttatt aatttcttta tcatatttga
    18541 atatcggttt aattattgtt aatgattgaa ttttttgaaa taaacataat aataaaataa
    18601 ttaagtatta ctttatttca aaacccatca tcatttttaa catcaaaact tatttctccg
    18661 tttgagtccg aaaatacatt cactgtaaaa gacctgcagc atacttcaca gtcagtgatg
    18721 aattcatctg aactgttatt gcttaattca atactcataa aatttacttc gccgcaatat
    18781 ggacagaaaa tttcaatgtc tttataaata atcaaaattg gatattataa attaaaacaa
    18841 taatatataa aagatatgaa atgatcagga ttatcccttc ccatctttca atttttcttc
    18901 ctttcccgac aaaaataaaa aggaataata aaatattggc ataaatattt acatagatct
    18961 cagtctgatt actgtcttct actgttaaag gagttatgaa tgctgaaatg ctgagtataa
    19021 aaaagatatt aaaaatatta gaaccgacaa tgtttcccag agcaatatct acattctttt
    19081 ttctaacagc gacaattgaa gttacaagtt cgggaagtga tgttcctgtg gaaacaattg
    19141 taagcgcaat aattctttct gaaataccaa aatctgaagc gattctgaca gcgttagtta
    19201 ctatcagttc accgccgatg acaagacctg caagacctat tattataaat aaaactgatt
    19261 taggcttaga ataatcttta gtttcaaatt gttcatcact ggtttctgat ttggcaactt
    19321 ccattgtata tacatgaaag atcaggaaga aacagaacag gattatcccg tcgctgctgc
    19381 tgattatatt actgcttgtt ttatcaagaa agatatcatt agaaatgatc agcaccatga
    19441 gtccggcgag aagtgaaaga ggaatttcca gccaagttgt atttcttttt acagcaatag
    19501 gataaaacaa tgaacagatt ccgagaatga ccagcacatt aaaaatatta ctgccgatga
    19561 catttcccaa aaccatttga gtgttgccat taacagctgc aacgaaattg acaaagagct
    19621 cgggtgcaga agtaccgaaa gcaactacag taagtccaat tacgatgttt ggaattccga
    19681 attttgacgc gagtgaagat gctccgtcaa cgagtttatc agcaccaaaa atcaggaaag
    19741 caaaacccag taataaaaga ataaaatcca actgtaattt ttttttatag attaaaatga
    19801 ctaagtagaa tcctcattga tccgtcaata aggatgagcg tagatataac gcctcctagt
    19861 gcacctccaa gatgtgcttc gtgaccgata tttccaagtt tttttttcat tccatagaca
    19921 gaaaaggcaa gccacagaaa agcaaatata taagcaggca ttggaataaa aaacacataa
    19981 agcatgcttt gaggaaagaa aataatatat gaaaagacaa ttcctgaaac agctcctgaa
    20041 gctccgactg caacatactg cggatcattg taatgaaata caactgacag cacagagccg
    20101 aacaacatac tgataaaata gatgagaaag aagtacagac ttccttcaag cgcaccgaga
    20161 ttatttataa aaaaagattc aaggtaagtt ccgaaagtaa acagtgcaaa catgttaaaa
    20221 aagagatgca taaaattagc atgcagaaaa gctgaagtga tcaactgata atattttttt
    20281 tcatgaacga tctgataagg ccattctgaa agtttataca taataattgg attgctgtac
    20341 attgcccata tagaaattgc aatgttggca aaaagtaaag taattgttac gggtgtttcg
    20401 gctaatgatt ccaaaattta aattgtttag ttcaatattt ttttaataat agacggacct
    20461 tcataaataa atcctgtata gatctgaaca agtgcagcac ctttctttat tttatccact
    20521 acatctgacg aattaaagat acctccgcat ccaataagaa taagtttata tcctgaattt
    20581 tttttgttaa gttcgttaaa aatattcaaa atttcatcag ataaaatttt aaccggtttt
    20641 ccactgagac ctcctgtttc atttatatct tcacttagac cggatctgct gactgtagta
    20701 tttgtagcaa ttatacctgt cagcttatgt gttacagcat tttcataaac agattgagca
    20761 tttatattat ttatgtcagg tgaaattttc agaaaaacag gtttgatatt ttttgaaaat
    20821 ttctgtgaca attcattatt cagtaactgg atttgtgaaa ggagctctga taaattttca
    20881 gactcgtgca atgttctgag cttttcagta tttggtgaag agatattcag agtaaaatag
    20941 tcagcgacat catataattt ttcgaaacag atcttataat cgctgatgga attttcaatt
    21001 ggtgtatctt tatttttacc gatattaata ccgatcacaa agtctttact taaagtcttt
    21061 ttagcaataa gaatgttttt tctgatctca tctgctcctt tattattaaa gccgagtctg
    21121 ttgataacgg cagaatcttt ttttaatctg aacattcttg gcttgggatt tccggattga
    21181 gacaatggag ttacagtgcc tatttcaata tgtgagaatc cgagtacatc ccagaacttt
    21241 attgcagcac catttttatc aaaacctgct gacagtccaa gtttatttct gaatgcgaga
    21301 tcactgacct ggataggttc attgttttta ggtgaatata aaatttttat taaaggatca
    21361 aaaattctga cttttgagaa aaagttcaac acacagttat gtgcggactc aggatccatc
    21421 ataaaaagaa atggtcttat aaaaatttta taaatcacaa tttataaaaa ggtaataaaa
    21481 gattaaaaaa aaagcccctg aaaattatcc agggactata ttaaaattaa gatttaaaac
    21541 atcagaagcg aaatactgaa tattatcatc ttcatcatta ttttgtcgga acggctggat
    21601 tcgaaccagc gacctccagt tcccaaaact ggcgcgatac cgggctacgc tacgctccga
    21661 taaaaacggt taaaaccgtt tttctaatta caagcgtgta agttaagcat aataaaaaat
    21721 ttattcaaac ccttttattt tctgattttg cattataaaa atcttaaatt acttttacga
    21781 aacagtatat tatttaattc tatattcaga ttctatttga agttggtcac gtctgctact
    21841 ttcactaaaa caacaacagc aattaacccc ttttattttc tgaatctatt tttaaataat
    21901 aaaaaaagcg gactcatttc gatccgcttt ttgtaaaaaa aaattaaaag aacacattaa
    21961 ttcatttcaa attaaaagtt tttgtttaag gctgtatttc tattgcaaac tggaacacgt
    22021 tattatcaca taaagcataa tctgatatat ctacaaattc ctcccagttt agatcggtaa
    22081 taacataatc tccaaataca aaattaaagg aatcattgtc tatggctgcc atatcagtga
    22141 tatctacgaa ttcatcttca tttatatcgc ctgtgaggta tgtagctttt cctgttacaa
    22201 gtttcatatt attcgcataa gctttattaa ctcccgttgt aaaatcataa ggagttaaac
    22261 ctgcaggcag taattctcca cctcctttgc tccaggtatt gattgaattt cggtgatcga
    22321 ctacaatgta ataggattca gaatcatcgg caaaaaagaa gtctatcgat tttttaccaa
    22381 gagtgtccaa atactcaact gctgtttcta taacattata tggcgatgta gctgatctca
    22441 gatttacggt taccgtatct ctgtcacgga aattaacatg accttctaca agtgcagtta
    22501 gatttaaagt tgacgggaaa ttgtcattct taatagatat accgtctaac cttaaatcac
    22561 cgaaaagacc tgccacttct atatgccgaa atgctatata gattggagtt ccgacataat
    22621 cttcaagact aacagctctt ctgtaatatg tattcggagt caaaagtccg agtgtaatat
    22681 ttacaggcaa gacaaaatcc agaaagtttg caactacgtt tccggtagtt gatactttca
    22741 cttcaagtct tcccagagct gtaataagat aatttgaact gacataaaat atcagtgaat
    22801 caccgcttac aggcgtgtac tgacttgaca tcatccagtt atccccgagc aaagttccgg
    22861 gagattcagc acacccgggc gaggtaagat aagaagaagt tgctcttgcc caggaaccgc
    22921 ttcctaaaag tccggaaaca tgaactgtag tccatcctgc aggcggaaaa gctaccggtg
    22981 taaaactttg tgtactgtga atgtttgaca tcgttttgac cggcaagcaa aataacatca
    23041 gaattaaaat agatttcaaa taatttgaaa tctgaaattt tgttaaaaac cccaaaacag
    23101 gattttccga aattttaatt aagtttaaaa ttttcatagt ttatcctttt tttttaatta
    23161 ataaaaattt ttttaaatta ttacaataac ctgacattca gacaagtatt aattcatttt
    23221 tgaactaaaa catgtaaaaa atgtgataaa acaagagttt tttcataagc taatatttaa
    23281 ttaatgatta attataaatc aactttacaa aagtaccttt ggggtatcgg caactaattt
    23341 ggagttataa cttagaagtt tgcagataat acatatctga aagatgaatg ttttgaatag
    23401 tattaaatgt taaaactttt aaagaaaata ttaaaaagtt tagtaaggtg tattttcata
    23461 tttttaattt aattggtttt taaatttttt ataatttttt aaaaacaatt tttaaatctt
    23521 ttttgatcta aaactcgggg aaagttttta atcttttttg aaacgttcat caatttaatc
    23581 tgaacttaat tttaaatata aaaattgtca agaccttttt tatgcacctt taaaaaagcg
    23641 aatgtttttt gaagaaaaaa cttcagtggg tttatttgaa aatggattct tgttattatt
    23701 tttatgaaat caatcagtag ttatgaaggt gataaattca atgtgaaacc ttttacactt
    23761 aaaaagtaaa tttttaattt aatttgtcgt gagtatgaga tagatttgga attttgacaa
    23821 atcacatttt ccaatgtcag atttacgttt gttgttgatt acgttcgctg tactgtctta
    23881 agacgtgaaa agcattctga aacagatttg gcagaacggg cattcatttc acatacttac
    23941 tcaaagtttc caatctcaat tttccgtttg ttgttgttca tgctcgcttc gctctcttaa
    24001 gaccaacttc ggcgaaaaaa attaaactaa gtctatagtt attcattatt gcggaaaaat
    24061 ttttgaaaca gtattcgtcc ttctgtaaaa tcaaaattct aactcgtaac tcgtaatttg
    24121 aaattgattt tattttatca aagccattcg tcttgtctca acaaaatccc cagcatctaa
    24181 tctgtaataa taaaccccgc ttggaaaatt atttccgtcg aaagtgactg agtaactgcc
    24241 ggcgttttga ttttcattta ccagagtcaa aacttcattt cccaaaatat caaacacctt
    24301 cagatttacc aactcgacac ctgtaacttg taactcgtaa ttgataactg tttttgggtt
    24361 gaatggattt ggaaaattct gtgacagctt atatcctgaa atttgtgatt gattattttg
    24421 tgaaatacct acgggagttt ccattaattt tctttcgtaa attccattac cgtgagtggt
    24481 cgcgcggagt tttctgtttg aataaactat cgtcagatcg aatacgagag tgtaaggcat
    24541 tcctgaactg tattcattcc agttcacacc gccgtttgta gtgacataaa ctccaagatc
    24601 atttccggca taaatattct gattgtaaac aggatcagct actacggatt gatgcggcac
    24661 attaggaagt gttccggaaa tgtcctgcca tgtacttccc gcattcaccg atctataaat
    24721 atgacccgaa ttatatccgc cgagtgtcag ataaacttcc gaagaattat ttggatttat
    24781 tgtaatatca gttacatatc tgttaggaat atttgtagaa atattcgtcc agttcagacc
    24841 gccattaaaa gatctgaaga cagtaccgtt tacaagtccg cagtataaag tatctgtgcc
    24901 ggtttgtgaa acggcaagtg ataaaatctt ttcattatta aaaactgaag tcccgtaagg
    24961 tccctgccag ttgccgccgc ctgtagtgga tttataaatg gactttcctc cgacatacat
    25021 tatctgagga tttgaaacac atgagataaa cggagttaca aagcaataat tatttccgtt
    25081 gccgctaccc ggaggtgaaa tgcctgacca gttcagtccg cggtcagttg atttgtacat
    25141 gcttccataa gtgtattccg tataacagat attatcattt tgtgaattta tcgcgcagct
    25201 cattccatct ccgaaaaatg tcttatacca cgcattattt ccctgataga atgtcgaacg
    25261 gttatcctgt aatccaccga gacagaatat agaatcctga tatgaatttg caaagcttgc
    25321 atagaattga gtagtcacaa gaccaccgtt acatgaaaaa aatgtttctc cgaaattatt
    25381 tgatctgtat aaacccccgt ctgcagcgat ataaacttta ttattatctt tcggatttga
    25441 tgcaaaaaaa tgaacatctg catgtacgaa attgtcagga ccttccggtt cacccggcgg
    25501 ggtagcacct tccacccaag cactccagtc ggattttgta ttaaacgaag atcctccgtt
    25561 gacagatttt tccacgtcta tagtaccaac gagcatagta ttgggatcat caggttttac
    25621 aatatgacca ttattatacc agccctgtga gccgatagga attgtcgtac ttagctgagt
    25681 ccagttcagc cctgcgtttg tacttttaaa atatccgaca taagaaaaat catttgaaat
    25741 acttgcatat acaaagtttt gatttccttt gtaaagttca agagtcgtct ttccagtcca
    25801 tgattcaggt agtccgccga taagttttat ccatgtggaa ccgctgttag tagatttgta
    25861 aattccttta tttgtctgag gtacattgtt tgtgagattg ccgatagatg cataaattac
    25921 agaagtatct gaattattca gctcaagatc cataaccatt ggataatcta aaacctgcgt
    25981 ccaggtggtt ccggcgttta cagatttata gattccttca cttgtacctg cgtatagtat
    26041 attagaattt ttattgttga tcaaaacttt ccagacacct ctttcattat tgtaagtcca
    26101 gtcgagagat tttgtccatg taattccgcc gtcagtagtt ttcagaattc caattccgta
    26161 catacctctt gttactctga cgtcgagacc attttttgaa aacatatatc cgtaattttc
    26221 acctgtgccg atatacatta catttggatt tgcggaatca attgcaatcg caccaatggc
    26281 agatgacgga aagccggtgt ttacaagggt ccaggcagtt gcgcctgttc cgccggtggt
    26341 tgatttccag agtcctccgg aggctgttcc gaggaaaacg acattcgtat caacaggatg
    26401 aattgcaatg ctgacagctc ttccgccgat gttattgggt ccgagtgacg tccattgatc
    26461 gttaccgtca ttgttattaa cctcttgtaa attttctttt gagtattcaa atgcctcata
    26521 atatttatca tgcggaatgt ctttttccgg ataagctctg atctgtgata tccactgcat
    26581 agattccatt gcacctgaga catttacttt tttttctttt gaataattat cttttgaatt
    26641 atctgaatta tctgaattat ctgaattatc tgaattttcc aaaaagccgg tagatttttt
    26701 cttgttggaa tttatatttt ccagcggttc agtttttgtt ttgtcaatta agagagacag
    26761 caggtaagtc aatccggcaa atacagcaat tgccagaaga aggattaatt tggatttcat
    26821 aataatgatt ataatttttt caattaactc attttataat ttttattaaa cataaaaatt
    26881 tttctttgtt atagaaaaaa ttaattggtg tatcagtatt ttaaatttaa tattcgagtt
    26941 ctgaaaatta aaaacaagat ttttgattct tttttaaaat aaaatattgt attaagtgtt
    27001 aagaattaaa ttcagatatt ctatctgtta gtcaaacaat aaacttataa aaatgaattt
    27061 ttaaaaatac atttatattg ttttgtttcg aattatttta tattgttaat acagtgattt
    27121 atattaaata tttacatact tagtccaagc ttatacaagg aattaagatg aagaattcaa
    27181 ttttgccaat cattacattc attcagaaat agttggaatt gccaccaaag cttcaagact
//
LOCUS       DAORVQ010000021        26805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_639_length_26805_cov_6.562168, whole genome shotgun sequence.
ACCESSION   DAORVQ010000021 DAORVQ010000000
VERSION     DAORVQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 26805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26805
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_639_length_26805_cov_6.562168"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..280)
                     /locus_tag="PLX80_03280"
     CDS             complement(5..280)
                     /locus_tag="PLX80_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19849.1"
                     /translation="MRYIKKISDKKVIENFYRKNTCLNVYPIGDLDDFFFTNTEWFAL
                     LEKQNDIEEIIEMAFIYRGTDLPVLIAVCDGDNLNLKEQDNNINDLK"
     gene            403..1638
                     /locus_tag="PLX80_03285"
     CDS             403..1638
                     /locus_tag="PLX80_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQY19850.1"
                     /translation="MEKVELQKYCDLIIKVGVNLYEGQCLAIGSGIKNADFALMLADT
                     AYANGAAYVDVIFSSNELTKSRIINSKNIDDINFIPNYPINRSYEQIANDWAFIRIDN
                     LEELNVLKSIDPNTLGIITKNEQTSHKSMSKAISSAKIAWCIVAAPGINWAAKVYDSE
                     PYIELENKLWDSLVKIMRLDKADPVSEWRRHGKNLSERGNMLSKMKIDKLYFTGPDTD
                     LEVGLTKNSIWKGGQVKAQNGREFIPNLPTEEVFTCPDFSRTNGKVKVTKPVKVLENL
                     LTGIWFEFKDGKVINYGADDKRDILEKFFEMDEGASYLGEVALVDINSEVHKSGLIFN
                     SILYDENAACHIALGRGIPMCFSNKDEIITTEDMKKNKCNYSVVHTDFMIGSDKINVK
                     AIDFEGKEIQIIKDGLFTI"
     gene            complement(1696..2322)
                     /locus_tag="PLX80_03290"
     CDS             complement(1696..2322)
                     /locus_tag="PLX80_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19851.1"
                     /translation="MKILILLIFIFSVSCGNKFQQIKEVKNIAGIDPCESPDADISCN
                     FENVPDNPGYVMNISSKTEPGIKIFIEGQILKADGKTPYPEILIYAYQTDSTGLYSKS
                     GNETGVQKLHGKLHGWCMTDINGKYKINTVRPGKYPTNDFPAHIHWAIREPDGKMQYL
                     NDFVFSDDKHVNEEYISKPYYKTGDVGVLKLSKNNNGIMWAKRITVLK"
     gene            complement(2333..3160)
                     /locus_tag="PLX80_03295"
     CDS             complement(2333..3160)
                     /locus_tag="PLX80_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19852.1"
                     /translation="MNNRINSPDYFLKLLAIVILITSTGCEQINKVLYKDAGTEADKK
                     VDENSNLKFIDSGKVNSKNESINTAIDSVVRTFEGLITINLNEKSFRDCSNPDSLYFI
                     KDETETIVNSYKKLNPMNDIYRSVFTSLKGFTVNADSTNNPDPEKYFKILVIKEVIAV
                     EGKNFRNTCIPYDYWCLGNEPNWSLQISKEENIFELTLPFEGKSYYFNYNEPKEEDGM
                     IKYFGYNNIQRTTLNISIKKENCSDTMSDKIYEYSVKIILNDKTNFSGCAIKGNFIE"
     gene            complement(3168..4016)
                     /locus_tag="PLX80_03300"
     CDS             complement(3168..4016)
                     /locus_tag="PLX80_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY19853.1"
                     /translation="MSDFLNTELIKTETFIITVWNLLNVLIIFIAAFIIIKVISTVLK
                     KSMLSKNKMDGRHQSVIQLIRYIIWIIAVSMCLKALHVDMTFLIASSAALLVGVGLGL
                     QNVFKDFISGIIILIEGTVKVEDIVEVEGIVLKVKEIALRTSKVVSREDKVMIIPNHK
                     FIEENVTNWTHNNTPTRFVLPVSVDYSSDTRLVEKTLHDCAMKHNDVIKEGRYAPYIR
                     FLDFGDSALLFDIIFWSNNLFRIESTKSDIRFDISDAFRANKIIIPFPQRVIHLPKEK
                     SEEELN"
     gene            complement(4018..5904)
                     /locus_tag="PLX80_03305"
     CDS             complement(4018..5904)
                     /locus_tag="PLX80_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY19854.1"
                     /translation="MKVIPLKLLLFFLLISGIAFSQVSENEKVIKEDSIKAQEDQVDS
                     LKVTEREILKQQEQNRIDSVLKVKLHEEMQLYSQDSQKKAELEEKLRKIEAEDSIKKV
                     EQLLKIKRLKEKARGFPVTLFTDTLFVMYTKLGSFDANERASTVTERVHSLYDEDFFS
                     PDSMKVDTGETTNDIIYNDKIIMSVSEVDALWFDKTKNEVSNEYLNTIKDTVVKYRND
                     NSFVNRLKRIGLALLTLIILFFIVKLIFKLFKKLKEYILERKDKYLSGLTIKDFKLLN
                     QQQSIKVAELILRILRLIVILLVLYFSLPVLFSIFPVTKSYAGLLLGWVLNPAKLVIS
                     AVIKFLPNLFTIAIIFIFTRYAIRAIKYFTDEIDAGRMELGNFHKEWAIPTFQIVKFV
                     MYTMMFVIIFPYLPGSDSPAFQGVSVFLGILVSFGSSSSIANIMAGLVITYMRPFKIG
                     DRITISDVTGDVIEKTLLVTRLRTIKNEDITVPNSLVLSGHTTNYSTNSKDTGLIVHT
                     TITIGYDVPWKKMHETLIEAAIRTEMILKDPKPFVLQTSLDDFYVSYQINAYTHEANK
                     QAVIYSDLHQNIQDTCNENGIEIMSPHYKSLRDGNLSTIPENYLPKDYIVPSFNIKEE
                     KDKR"
     gene            complement(5947..6165)
                     /locus_tag="PLX80_03310"
     CDS             complement(5947..6165)
                     /locus_tag="PLX80_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HQY19855.1"
                     /translation="METSLILISNITCPECGFIKEEKMSENSCLWFYECHKCKTVLKP
                     KSGDCCVFCSYGSVKCPPIQSDGFCCKE"
     gene            complement(6359..8302)
                     /gene="uvrC"
                     /locus_tag="PLX80_03315"
     CDS             complement(6359..8302)
                     /gene="uvrC"
                     /locus_tag="PLX80_03315"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQY19856.1"
                     /translation="MTDSRDKIEFNDDKELTPTLKEKLKNLPTSPGVYQFKDSTGKLL
                     YIGKAAILKNRVRQYFQAKPVSPMLNAMIRKISDIELIATDNEVEALILEQNLIRTFK
                     PRYNINFKDDKSYPYIVITNEPFPRIFPTRSKRSDGSKYFGPYTDVKNMRYALKTVRN
                     IFMIRSCSLNLTEDSIAQNKFNLCLDYHIQKCEGPCVGYVSREEYNKTIDHVSKLLNG
                     KTKDLISDLTMQMKEYSDKMQFEKAKVFRDKINAIEVYSSKQKMVDDEIIDRDIFAFE
                     KTGNDSCGMILKIRDGKVIGRTHYFLSNVEDKSQSEILEQLIVNYYSKSEFIPDEIYL
                     MNELDNENTIKDWLEKKKSGKVNFVIPKIGDKYKLVFMVRKNARLMLDELILSKLKRE
                     FIPPSLDSLKRDLNLSKLPRRIECYDISHIQGTDTVASMVVFVEGRPKKSDYRKFKIN
                     YVTDEAGNPDDFLSMREVIYRRYKKIVEKSTGDNTAMENTINSNVKEDLSFGSVPDLI
                     IIDGGKGQLSSAVKVLDDLGVKDQNIIGLAKRLEEVYLPGESMPHNISKTSSGLRLLQ
                     RVRDEAHRFAVTYHRSLVDKRTLTSELSEVEGIGEKTAQKLLTKFGSVENIKVLLKDS
                     YDQFEKEAGKKMADRLRTFFEIK"
     gene            complement(8336..8734)
                     /gene="mce"
                     /locus_tag="PLX80_03320"
     CDS             complement(8336..8734)
                     /gene="mce"
                     /locus_tag="PLX80_03320"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQY19857.1"
                     /translation="MKLAHIGIAVKSLEESVKVFEKIFNSTASDTEFVEEQKVNVRKF
                     HLENCEIELLEGTDPESPISKFIEKKGEGIHHVSFGVKDISGSLDKLKEEGIGLINET
                     PRTGADDMLIAFLHPKSTNNILIELTQKKE"
     gene            complement(8759..9865)
                     /locus_tag="PLX80_03325"
     CDS             complement(8759..9865)
                     /locus_tag="PLX80_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQY19858.1"
                     /translation="MINYAVIMAGGVGTRFWPKGNSKVPKQFLKITDDDDTLLQLAYK
                     RLDGVFKNTNIFVVTNVIYKQEVKRQLPKIPEENIICEPVGRNTAPCIGLSCVFINRF
                     QPEANVLVVAADHLIGNVDEFHRVIKSGLKFVNDNGGIVTLGIHPTLPETGYGYIQYN
                     SDIYEDVEIESKNDTSTERIYKVKTFAEKPNMEVAKAFLESGDFLWNSGMFIFRTDTM
                     MQEIERFMPELHESLNRLSDNILSTQFPKVLEDEFAKLKSISIDYGVMEKSENVHIIR
                     SHFGWNDVGAWDEVYNIKEKDPDGNVRKGMTVTEDTKNCLIINDMKIVATIGVEDLLI
                     INTENGLLICKRGEAQRVKEVVDYLRRKGMDQYL"
     gene            complement(9878..11992)
                     /locus_tag="PLX80_03330"
     CDS             complement(9878..11992)
                     /locus_tag="PLX80_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19859.1"
                     /translation="MSESRNKDYINFIFKKSINDLGKYNVGEKQFQNINEIIDKLLRS
                     ENLLRDLYILYHIKEFRNTGKYLIYILKKLDDNLITFDNLTQNAKEDSEFIHKEFLKY
                     LSGERSESVKVNNKKVFDLNGITEEDNSGNEIIDVESELIKQDQLTEIVDEEDDEEMM
                     TFKQNYMELIQSEEENDNVAFILPGLENEEQTSAEGMVEEDPESDLPEMPDLNENDLT
                     ESEVDKTNVNANTIAVNDNVKESLSVDENLQSKSEENTSGKNSTQEEDENVSNSGDLS
                     EGNHEVNFNKLKDLNYDDSGEYFIIKKVLPAAGINNSEESDEDTEDTEDTEELNTNDS
                     DISKEEPMNDPAGDYENSEQEEKMNLNSDIQEELNKYEKELKEKLLDSASGHKEGSGE
                     SGYSDESVRSTDSEKEEYIVEQKANEEFIEFETEIKSLNGKLNSEFDSLIYIITAKID
                     NEEERNNIISNIRDISDNLELNSKEMSLEIISNIYQAIRLSFEKIAKGKYDITESTLN
                     LFKQGLILVSNLIKGEDYFGYKNILKSIENIRKGLIEENKKREEFIKQRDLKKEIETK
                     LDERFPEEDQKEKLINLKKLIKDTDENFKALYKIEGEFQIYEALRSLSTNLNNLKETV
                     LIAKDLKLTKMVQLSEAGYIFLKFLNSYRINPVTDDIKEIFSYFIYNMKALIIDKEVE
                     DQDVFISYLNDPVKIFNKIKKN"
     gene            complement(12003..13160)
                     /gene="alr"
                     /locus_tag="PLX80_03335"
     CDS             complement(12003..13160)
                     /gene="alr"
                     /locus_tag="PLX80_03335"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQY19860.1"
                     /translation="MNLKTAEKNFSDTFAEIDLKIFNKNFLKVRKYACRKNADTKICS
                     IVKANAYGHGMNVMAESLIGSGTDYLGTADYTETSELRNYLDSKKLYKTPVLCLGIIT
                     EKNKLLEDLACKNVEFSVADTSSAVILNKAAAKLNTKISIHIQVDSGINRTGFRMNDA
                     YDAVKEITSMKNLKLKGIYSHFATSEIPGNLFAKKQEAEFRKLVKSLEESIIKFELRH
                     ISNTGGILNFNDGYFNMVRPGISLYGFYPDEKKVKNGIGIEPVMTLKSKVKFIKQLEK
                     GESISYGRTYFTKNKTRIASVPIGYGDGYIRSFSNKAKIFIKGKLYNVTGTVCMDWIM
                     ADIGMKNEINVNDDVEIFGRNYPAYKLAALAETISYEITCNVSSRVKRIYI"
     gene            13580..15145
                     /gene="dnaA"
                     /locus_tag="PLX80_03340"
     CDS             13580..15145
                     /gene="dnaA"
                     /locus_tag="PLX80_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQY19861.1"
                     /translation="MINFSTADSQKDNDKNKNSISGNEELKLSEESRQTELIWKKFLE
                     LLSDNLKAGEINTWFSVIIPKSYKDNVLTISVPSEDYYSLIESRYNKIISSITETLLG
                     SDGKLNYEISQYGLFGDNESENIAKKNQILEKKKPAFGEINIFDSGVSSNYYSNDNSN
                     VNSDSHKNFSTNLNKKHIFENFIKGESNELAVAAAYAISNNPGKTYNPFFVYGGVGLG
                     KTHLIQAIGNEILKKNPDMKVYYTTAPDFTTEFTTNIAQNRIDFSSNRNGTKKLDSFY
                     KSLDVLILDDVQYLSGKEKTQDFMYQIFNTLYNDKKHIIFSSDKPINQIKGVEERLMS
                     RFQWGITTDIQAPNWEMRVAIIQKKLMEADIEAPEEVVHFIASNVKDSIRTIEGCIVG
                     IIADSVLRYKGEINLEIAENVISRVIGTVKRAKNISIENIIYAVANYYRISENQILSR
                     KRTKEIAFARQVAMHLAKELTPLTLESIGLNFGGKDHATVLYANNIISNLIKKDKDVL
                     NQILEIKDHLKNL"
     gene            complement(15140..15772)
                     /locus_tag="PLX80_03345"
     CDS             complement(15140..15772)
                     /locus_tag="PLX80_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQY19862.1"
                     /translation="MVFNKKDKIIYNKDIDNLFKKDKIFSQIHDAYGSPENVIRPQGF
                     ITLSKIIIGQQVSLESANSHFLKLSDFVQEFTPAQIIKLSDTEMRNCYITRQKSEYLR
                     SLSNAILNNELDLENLSGQDQNKTREILKKIKGIGDWTADIYLMFALQAKDIFPAGDV
                     AVINAAIELTGCKTKIEVVKFSEKWKPYRSLASFFLWHYYLKKRKRELLL"
     gene            16193..17905
                     /gene="recN"
                     /locus_tag="PLX80_03350"
     CDS             16193..17905
                     /gene="recN"
                     /locus_tag="PLX80_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQY19863.1"
                     /translation="MIEKLLIKNYLIIKEAEIHFSEGLNILTGETGAGKSIILDALSL
                     LLGERADHSKIKSGDDKLIMEGQFNFTDNKIVENLYKEMFPEDEFNGYLILRREINTK
                     GINRSFINDTPVNISDMKKFGDVVIDIHSQNEHQSLLSKETHIGILDNYVSDETIFSE
                     YEKGYLDLRKSVSDYKEFISKKEDLLSRKKFLDHDLKEINNVNIQPGEDEEIETELDR
                     LENSEEISSSLSISLEGLSENDFNALNIISAAVKELKKISDYDKNFEKLIEDLESSYI
                     LIKETSSSLLNYNNDLNFDPVRIEHLRSRLLAINSLKRKHELDIPELLEKAENIQKDL
                     TFAENFDHEIDKLDKKISVMKSELFSLAEKISDKRIKRAKDLEKEINKLLKEVGLESA
                     EFKVDIKKNISDSENEFTVKKGKENYELSQNGINNIEFLIRTNKGMELSPLRKSASGG
                     EISRIMLAIKTVLSENDNIGILVFDEIDAGISGRIAQKVGNTLKKLAVSHQIICITHL
                     PQIAANSDRHFNVSKSDENNETKAYINILDDNEKINEVAKLLSGEKITESTINSAKEL
                     ISGK"
     gene            complement(18057..19052)
                     /locus_tag="PLX80_03355"
     CDS             complement(18057..19052)
                     /locus_tag="PLX80_03355"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HQY19864.1"
                     /translation="MLLIDNQNITDPTFNLALEEYCVRNLNMENEYLLFYINEPSVII
                     GKHQNTIEEVNNDYVKQNGVHIVRRISGGGAVYHDKGNLNFSFMTKHSHQSIHNFKLF
                     TEPVIKVLQEMGINAELNGRNDITVSERKISGNAQFTDTKSMFSHGTLLFNSNLDNVV
                     KALNVQTDKIESKGIKSVRSRVANIKEFINIDMSVYDFKDIITESIFGSKENIKFYTL
                     SDDELEGVKKLSESKYRSWDWNYGRSPEFNIQKKKRFDFGQIDARIQVKDGIIKEFKI
                     FGDFLGHGELSEVENKLTGLKYDNISIKEGISDIDLKHYLGNITSQEFANFLTEI"
     gene            complement(19059..19436)
                     /locus_tag="PLX80_03360"
     CDS             complement(19059..19436)
                     /locus_tag="PLX80_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19865.1"
                     /translation="MNKINDSKNPPKKERLWLFLIAGIIAAGVIYMYMDSQNHKDPTF
                     HEWSAEMDSVFVKNCYDKYKPQIKDDMGKQEIMKSFCKCMLEKIKTRYSEGEMNKVTG
                     VEIKEWDEQCRAQLTNPIIPENK"
     gene            19596..20057
                     /locus_tag="PLX80_03365"
     CDS             19596..20057
                     /locus_tag="PLX80_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19866.1"
                     /translation="MIPQFNNLDQKETELMLDAPALVTLLIAGSDGNIEQKQVDWGSK
                     ITHFRAKDEDSIMQSYYIEVEKSFNDTLAQFINAFPNDIEERGKLINNELIKINDILP
                     KLNKDFAKQFYSDMKSLSKQIAKATGGVWGYGSISAEEQKYLDLDIINPVE"
     assembly_gap    20153..20252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20253..21561)
                     /gene="rmuC"
                     /locus_tag="PLX80_03370"
     CDS             complement(<20253..21561)
                     /gene="rmuC"
                     /locus_tag="PLX80_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQY19867.1"
                     /translation="MESSGNILYLVIALISGFFIAWIIIKLISEKDKGLSLKEIESNY
                     IPKDLYENEKKNLAIKESEILELNRSLARTEEHSKNLTEKLEVQKSDMEDLHNIFKTE
                     FKNLSNELLEEKGKKFTELNEKNIGDILKPLKEKISDFEKKVDDTFKEETRERISLKK
                     ELEQIVKLNQQVSEDTNRLTGALKGDKKLQGNWGELQLELILEKAGLEKDIHYRKQET
                     LLDAGGNVFRPDYIINLPEDRNLIIDSKVSLVAYENYFNSENDNDKASFLKEHLKNIM
                     NHISDLSSKNYPKLYGINPPDYVIMFVPIEPALHSVLKENTEMIERALEKNVLPVSTS
                     TLLATMKTISFIWKQERQRKNVIEIANESGALYDKFHGFINDLVTLGNKIDDSKENYS
                     SAMNKLFESKKKGDTIIGRIERIKKLGAETNKSLPENILRRLEE"
     gene            21774..23900
                     /locus_tag="PLX80_03375"
     CDS             21774..23900
                     /locus_tag="PLX80_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19868.1"
                     /translation="MNKNLKYFYGIFSLLILTLICSIIFMKCTDDSDDRDKVLSKLHE
                     KYYYLNLYEDVKYTGTEKCVSCHNDIGETFHHTGMGSSLYKPEAGNEIEIYEKDNIIY
                     DGKNDLYYEAYRKDGNYFQREFRKDENGNLIHSLEKKVDYIIGSGFNTRSYLYQDNGF
                     FYEMPMSWFTEKSKWDLSPGYQRLNLRFRREINQECMNCHNSYSGYFEFSENKFKMPI
                     AEGIGCERCHGPGELHVRRFTDKDNFFTAIESDTIDRTIVNPADLPLEEKLSVCFQCH
                     LQGDVRVFTDGKKQSDFRPGMKLSDVKTVFVQDDVSEGDFKIASHAERMFLSSCFKNS
                     DGGMTCLTCHDPHVTVGNSTKEFFNNKCLGCHQQNSLSPSKTNVDHKAGSNCISCHMK
                     QGNTSDILHVNFTDHWIRKKTGVESDKISGDENSQADKSVIPVTLKNFEFTVNEKNKN
                     SGLELGIAYVIYYETKHPHQEYLKRAQPLLEAGVKTDPENRSALYYLGSAYYLDNKYS
                     ESVNILELLIAKDTGNAEAFIRLGAAYEKSGNTQKAIESYEKALNIFPENVKLLNSLG
                     NVYYSSGKINEAVETYKRGIKIIKDNPIILTNLGDISLYKLENPDAAFNYLNKALEYD
                     PDKKETLNNLGNAYLITGNKNEAEKIFKRVLKLDANNALALGNLAVIYEDNGNKNEAV
                     KMLKKILENDPNDKRAKEMLSKLKGK"
     gene            complement(24049..25410)
                     /locus_tag="PLX80_03380"
     CDS             complement(24049..25410)
                     /locus_tag="PLX80_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY19869.1"
                     /translation="MNKILTLIMLFAFYGISLSQSVSNMQMLGSANEYTAGGTPGGWH
                     YAACWGYVANGREYAIIGHYAGTTVYDVTNPDNIINKGTVAGPGSFYNYREMKTYSHY
                     LYIVSEGGSGVQIVDLQYLPDSIHFVKNYTFTGYTRSHSISQDGRFLYSNGGNYNNGG
                     VFILDLLDPENPVKRGQWGTRYVHDCFIKNDTIYAASINQGFMVFLDATNKDSIKFIR
                     EFTYPSAVTHNAWTTDDRNILVTTDEGGTNHAILWDISDFSNQVRLSEIIPYDSASMV
                     HNAYIKGDSLYLSHYRAGVIVYDISNPSLPVEVGHYDTYPGTNSTAYQGCWNVYPYLP
                     SGNIIASDISTGLYVLKVGNTTSINNNSTEIADNYTLSQNYPNPFNPLTNITYYLPNQ
                     EFVKITVTDELGRQVRTLVNEVQQKGSYSAEFKADGLASGVYFYTINAGNFVSTKKMI
                     LTK"
     gene            25676..26311
                     /locus_tag="PLX80_03385"
     CDS             25676..26311
                     /locus_tag="PLX80_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQY19870.1"
                     /translation="MNTASTIVQKIVITLRKEITNGILKSGAHLTETSVSKNFNVSRV
                     PVREAFRILQSEGYIDLIHNRGGFVKSVSRDNAIENGEIYVLLVPMILKKAIPNYTKS
                     TFKKAHAILDKIENCEKYCDSSYLVWDFAKVIYSPSKMELSMKIINDIYSQGIRALND
                     YFEIEDTTKVTIDSHREFVNLCEIKKFDEAAELWINHVEHVKERIINSLPD"
     gene            complement(26359..>26805)
                     /locus_tag="PLX80_03390"
     CDS             complement(26359..>26805)
                     /locus_tag="PLX80_03390"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQY19871.1"
                     /translation="EVQALVTSSNYGIPQRTSMGFDYKKLNIIVAVLEKKLGIFLNKS
                     DIFLNIAGGIKIEEPATDLAVAMSIYSSFRDIPVDSETVLLGEIGLSGEVRTISYAER
                     RITEAAKLGFKRIIIPKGNLKNINSKKFNAEVTGVEKINDAVDKLF"
BASE COUNT         8583 a         4556 c         4155 g         9411 t
ORIGIN      
        1 ggagctattt taagtcattt atattattat cctgttcctt caaatttaaa ttatcaccat
       61 cgcaaaccgc gatcagtaca ggcagatctg tgccgcgata aataaatgcc atctcaatga
      121 tttcttcaat atcattctgt ttttctaaaa gtgcaaacca ttcagtattt gtaaaaaaga
      181 aatcatccag atcaccgatc ggatatacat tgagacaggt attttttctg taaaaatttt
      241 cgattacctt tttatctgaa atttttttta tatatctcac attcggtttt taactgtttt
      301 aagttaaaga aaaaagttgg ccttttttaa aaaactcttg attagaatta aatttaaaaa
      361 tcaatttagt taacttaaaa aattaaaaaa atttaataaa taatggaaaa agtagaatta
      421 cagaaatatt gtgatctgat aattaaagtc ggcgtgaatt tatatgaagg acagtgcctt
      481 gctattggca gcggaattaa aaatgctgat ttcgctctga tgcttgctga tacagcatat
      541 gctaatggtg ctgcttatgt cgatgttatt tttagttcta atgaacttac caaaagccgc
      601 attataaatt ctaaaaatat tgatgatatt aattttatac caaattatcc gataaaccga
      661 agctatgagc agattgcaaa tgactgggct tttatccgga ttgataatct tgaagagtta
      721 aatgtcctca agtcaattga tccaaacaca cttggcataa tcacaaagaa tgaacagact
      781 tcacataagt caatgtcgaa agcaatttca tcagcaaaga ttgcatggtg catagttgct
      841 gctcccggaa ttaactgggc tgctaaagtt tacgattctg aaccttatat agaacttgaa
      901 aacaagctgt gggatagtct ggtaaaaatt atgcggttag acaaagctga tcctgtaagt
      961 gagtggagac gtcatgggaa aaatctttca gaaagaggga atatgctttc aaaaatgaaa
     1021 attgataaat tgtattttac gggacctgat actgatctgg aggtcggact tacaaaaaat
     1081 tctatctgga aaggcggtca agtcaaagct cagaacggca gagagtttat tccaaacctt
     1141 ccgactgaag aagtttttac atgtcctgac tttagccgga caaatggtaa agtaaaagtt
     1201 accaaacctg tcaaagtttt ggaaaatctt ctgacaggaa tctggtttga attcaaagac
     1261 ggaaaagtaa taaactatgg agcagatgat aaaagagata tccttgaaaa attttttgaa
     1321 atggatgaag gagcttctta tcttggggag gttgcattgg tcgatataaa ttccgaagta
     1381 cataaatccg gtctgatttt taacagtatt ctttatgatg aaaatgccgc atgtcacatt
     1441 gcactgggga gaggaattcc tatgtgcttc tcaaataaag atgagatcat tacaactgaa
     1501 gatatgaaaa aaaataaatg taattattcg gtagttcata ctgattttat gatcgggtct
     1561 gataagataa atgttaaggc aattgatttt gaaggtaaag agatacagat aattaaagac
     1621 gggttgttta caatataatt ctttgcaaaa attttttaaa ttaacttccg gagtttttct
     1681 gactttggaa gtttattatt ttaaaaccgt tattcttttt gcccacataa taccgttatt
     1741 attttttgaa agttttaaaa ctcctacgtc accagtttta taatacggct ttgaaatata
     1801 ttcctcattc acatgcttgt catcagaaaa aacaaagtca ttaagatact gcattttacc
     1861 gtcaggttcg cgtatagccc agtgaatgtg tgccggaaaa tcgttagtcg gatattttcc
     1921 gggtcttact gtattaattt tatattttcc attaatgtca gtcatacacc aaccatgaag
     1981 tttaccgtgc aatttctgaa caccggtttc atttcctgat ttagaatata atcccgtaga
     2041 gtcagtctga taagcataaa ttaaaatttc cggatatgga gtctttccgt ctgcttttaa
     2101 aatctgtcct tcgataaata ttttaattcc gggttcggtt tttgatgaaa tattcattac
     2161 atatccggga ttatccggaa cattttcaaa attgcatgaa atatctgcat caggactctc
     2221 acagggatct atgcctgcaa tattttttac ttctttgatc tgctgaaatt tatttccaca
     2281 ggaaacagaa aatatgaata ttaaaagaat taatattttc atataaatta ttttactcaa
     2341 tgaagtttcc ctttatcgca caacctgaga aatttgtttt atcgttaaga attattttta
     2401 ctgaatattc ataaatttta tctgacattg tatctgaaca attttctttt ttaatggaaa
     2461 tatttaatgt cgttctctga atattattat atccaaaata ttttatcata ccatcctctt
     2521 ccttcggctc attataatta aaataataag acttaccttc aaaaggcaga gtcagctcaa
     2581 aaatgttttc ttctttggaa atctgaagtg accagtttgg ttcattacca agacaccaat
     2641 agtcataagg tatgcatgtg tttctgaagt tttttccttc aacggcgata acttctttaa
     2701 taacaagaat tttaaaatac ttttcaggat ccggattatt cgtactatct gcatttacag
     2761 taaatccttt taatgaggta aatacggatc tgtaaatgtc attcattgga tttaattttt
     2821 tgtaagaatt aacaatcgtc tcagtttcat ctttaataaa atataatgaa tccggattgc
     2881 tgcaatccct gaatgatttt tcgttcaggt taattgttat aagtccttca aaagttctga
     2941 ccacagaatc tatagcagta tttattgatt cattttttga atttactttt cccgaatcta
     3001 taaattttag gttagagttt tcgtcaactt ttttatcggc ttcagtaccc gcatctttgt
     3061 acaaaacttt atttatctgc tcacatccgg ttgaagtaat taatattaca attgctaaca
     3121 gttttaaaaa atagtctgga gaattaattc tattattcat gtttaaatta atttaattct
     3181 tcctcacttt tttcctttgg taaatgtatc actctttgtg gaaacggtat aataatttta
     3241 tttgctctga aggcatcaga tatatcaaac cgaatatcac ttttagtact ttcaatccgg
     3301 aataaattat tactccagaa tattatatca aaaagcaaag ctgaatcgcc aaagtcaaga
     3361 aaccttatat aaggtgcata tcttccttct ttaattacat cattgtgttt cattgcacag
     3421 tcatgaagtg tcttttctac taatctggta tctgaagaat aatcaactga aaccggaagt
     3481 acaaatcttg ttggtgtatt attatgtgtc cagttcgtaa cattttcctc tatgaattta
     3541 tgatttggga ttatcattac tttatcttca cgagatacta ctttagatgt acgcaatgcg
     3601 atctctttta cttttaatac gattccttct acttccacaa tatcttccac ttttactgtt
     3661 ccttctatca gaataattat tccgctgata aaatccttga atacattctg cagaccgagt
     3721 cccacaccta caagtaaagc tgccgaactt gctatcagga atgtcatatc tacatgaagt
     3781 gctttcagac acattgaaac tgctatgatc catattatat atcttataag ctgaattact
     3841 gactgatgtc ttccgtccat tttgtttttg gaaagcatgg acttttttaa aactgtagat
     3901 attactttga taataataaa tgcagctata aaaattatta atacattaag taaattccaa
     3961 actgtgatta tgaatgtttc tgtttttatt aattctgtat ttaaaaaatc tgacatctta
     4021 tcttttatct ttttcttctt ttatattaaa tgaaggtact atgtaatctt taggtaaata
     4081 attttcaggt attgttgaaa gattaccatc tctcagagat ttgtaatgcg gagacatgat
     4141 ctcaattccg ttttcattgc atgtatcctg aatattctga tgaagatcag aatagataac
     4201 cgcttgtttg tttgcttcgt gtgtataagc attgatctga taggatacat aaaaatcatc
     4261 cagacttgtc tgaagcacaa atggctttgg atcttttaag atcatttccg ttctgatcgc
     4321 tgcttcaata agtgtttcat gcattttctt ccatggtaca tcatacccta ttgttatagt
     4381 tgtatgaact atcaagccag tgtctttgct gttggtagaa tagtttgtag tatgacctga
     4441 cagaacaaga gaatttggaa cagttatatc ttcatttttt attgttctta atcttgtaac
     4501 taataaggtc ttttcaatta catcacctgt cacatctgat attgtaattc tgtcaccgat
     4561 tttaaatggt ctcatatatg taattacaag acctgccatg atattcgcaa ttgatgatga
     4621 tgatccgaac gaaaccagta ttccaagaaa tacagacact ccctgaaatg ccggggaatc
     4681 tgagcccgga agatatggaa atattatcac aaacatcatc gtatacatta caaactttac
     4741 tatctgaaaa gtcggtattg cccattcttt gtgaaagttt cccagttcca ttctgcctgc
     4801 atcaatctca tccgtaaaat atttaattgc tctgatggca tatcttgtaa agataaatat
     4861 tattgctatt gtaaaaagat ttggtaaaaa tttgataact gcacttatta caagctttgc
     4921 cggattcaaa acccatccca gtaataatcc cgcataactt tttgtaactg ggaaaatact
     4981 aaacaatacc ggcagactga aataaagaac taacagtatt actatcagtc ttaaaattcg
     5041 taaaatgagc tctgctactt ttatgctctg ctgctgattt aaaagcttaa agtcttttat
     5101 tgttaatccg gacaggtact tgtcttttcg ttctagtatg tattccttta gctttttaaa
     5161 taacttaaat attaatttaa ctataaaaaa taatattata agagtaagca atgcaagccc
     5221 tattcttttt agtctgttca caaaactgtt atcatttctg tactttacaa cagtatcctt
     5281 aattgtattc agatattcat ttgaaacttc attttttgtt ttgtcaaacc aaagcgcatc
     5341 cacttcagat acactcataa ttattttgtc attataaatg atgtcattag tggtttcacc
     5401 tgtatccact ttcatggaat caggagaaaa gaaatcttca tcatataatg aatgtaccct
     5461 ttctgtaact gtagaagctc tttcattggc atcaaatgaa cctagcttcg tatacatgac
     5521 aaataatgta tcggtaaata atgtaacagg aaatcctctt gctttttctt taagtctttt
     5581 tattttaagt aactgctcta ccttttttat tgaatcttct gcttcaattt ttctgagttt
     5641 ttcttccagc tcagctttct tttgtgaatc ttgagaatat aactgcatct cttcatgaag
     5701 ctttacctta agcactgagt ctattctgtt ttgttcctgc tgttttaaaa tttcccgttc
     5761 agtgactttt agagaatcta cctgatcttc ctgagctttt attgaatctt ctttaattac
     5821 tttttcattt tcacttacct gcgaaaaagc aattccactt atcagcagaa aaaagagaag
     5881 taattttaaa ggtataactt tcataaatag tattttaccg ggtgagcagt tttttaaata
     5941 aataaattat tctttacaac aaaatccgtc tgactgaata ggtggacatt ttacagatcc
     6001 ataactgcag aatacacagc aatctcctga ctttggtttt aaaactgttt tacatttatg
     6061 acattcataa aaccataagc atgaattttc actcatcttt tcctctttta taaaaccgca
     6121 ctccggacat gtaatgtttg aaattaatat taaacttgtt tccatatgat gtaataattc
     6181 ataccttaat tgatcagcta gttgaaatta cgcaattatt gatatttgca acaatcggat
     6241 gatgctcgtt tccaaactcc gtttggaaat gtaattttca tgcaaaactc aatttcgcaa
     6301 aagttcttta aaatgaaatc ctcaataaaa ttaaaaatgc gtaacttcag tcagttaatt
     6361 atttaatttc aaaaaaagtc ctgagtctgt ctgccatttt tttacctgcc tctttttcaa
     6421 actgatcata actgtcttta agcaagactt ttatattttc cacagatcca aatttagtaa
     6481 gaagtttctg tgctgtcttc tcgccaatac cttctacctc agagagctca gatgtcaatg
     6541 ttcttttatc tacaagactc ctgtggtatg ttactgcaaa tctgtgcgcc tcatccctta
     6601 ctctctgcaa aagtcttaat ccgcttgatg tcttggaaat gttatgtggc attgattctc
     6661 cgggcagata tacttcctca agacgctttg ccagtcctat aatattctga tcctttactc
     6721 cgagatcatc caaaactttt actgcagatg aaagctgacc ttttccgccg tcgattatta
     6781 tcagatccgg aactgaacca aaactaagat cttctttgac gtttgaattt attgtatttt
     6841 ccattgccgt attatcacct gtactttttt caacaatttt tttatacctt ctgtaaatta
     6901 cttccctcat agaaagaaaa tcatcagggt ttccagcttc gtccgttaca taatttattt
     6961 taaattttct gtaatctgat ttcttcggac ggccttccac aaatactacc atcgatgcaa
     7021 cagtatccgt accctgtata tgtgaaatgt cataacactc gatccttctc ggaagcttac
     7081 tcagattcag atcccttttt aaggaatcaa gtgacggcgg aatgaattct cttttgagct
     7141 ttgataaaat aagttcgtca agcattaatc tcgcattctt tcttaccatg aaaacaagtt
     7201 tatacttatc accaatcttt ggtattacaa aatttacctt tcctgatttc tttttttcga
     7261 gccagtcttt tatagtgttt tcattatcaa gctcgttcat cagatatatt tcgtcaggta
     7321 tgaattcaga ttttgaataa taatttacaa tcaactgctc taatatttca gactgtgact
     7381 tgtcctctac atttgaaaga aagtaatgtg tccttccgat tacttttcca tcccttattt
     7441 ttaatatcat tccgcaactg tcattccctg tcttttcaaa agcaaatata tctctgtcaa
     7501 tgatctcatc atccaccatt ttttgtttcg atgaataaac ttcaatagca tttatcttat
     7561 ccctaaacac ctttgctttt tcgaactgca ttttatcaga atattctttc atctgcatag
     7621 tcagatccga aatcagatct tttgttttcc cgttcaggag tttggataca tgatcgatag
     7681 tcttattata ttcttccctg cttacgtatc caacacacgg tccttcgcat ttctgaatgt
     7741 gataatctaa gcaaagattg aatttatttt gagctatcga atcttcagtc agattcagac
     7801 tgcatgacct gatcataaaa atatttctca ctgtcttcag agcatacctc atatttttta
     7861 catcagtgta aggtccgaag tattttgatc cgtcagatct tttacttcgt gtcggaaaaa
     7921 ttctcggaaa tggttcgttt gtaattacaa tatacggata tgatttatca tctttaaaat
     7981 tgatattgta tctcggctta aaggtcctga tcagattttg ctcaagtatg agagcttcga
     8041 cttcgttgtc ggtagcgatc agttcgatgt cgctgatctt tcggatcatt gcatttaaca
     8101 tcggcgatac aggctttgcc tgaaaatact gacgaactct gttttttagt attgccgctt
     8161 tgccaatgta aagaagtttg ccggtggagt ctttgaactg atatacaccc ggtgaggtcg
     8221 gaaggttttt taatttttct ttgagtgtcg gtgttaattc tttgtcatcg ttaaattcta
     8281 ttttatctct gctgtcagtc atttattaaa gttactgtaa ttattttata aagaattatt
     8341 ccttcttttg tgttaactca attaaaatat tgtttgtaga tttcggatga agaaaagcga
     8401 tcagcatatc gtcagcacct gtccggggag tttcgtttat cagaccaata ccttcttctt
     8461 tcagcttatc caatgatcct gaaatatcct ttaccccaaa agatacgtga tggatgcctt
     8521 ctcctttttt ttcaataaac tttgaaatag gtgattcagg atctgtacct tcgagaagtt
     8581 ctatttcaca attttccaga tgaaatttcc ttacatttac tttttgctct tctacaaact
     8641 ccgtatctga agctgttgaa ttaaatattt tttcaaaaac ttttacagat tcctcaagtg
     8701 actttactgc tattcctata tgtgcaagtt tcatatattc taaagatcaa aattattttt
     8761 acagatactg atccatccct ttacgtctga gataatccac aacttctttt actctctgag
     8821 cctcacctct tttacagatc agaagtccgt tctcagtgtt aattataagt agatcttcta
     8881 cacctattgt tgcaacaatc ttcatgtcat ttataataag acaatttttt gtatcctccg
     8941 ttactgtcat accctttctt acatttccat ctggatcttt ttctttaata ttataaactt
     9001 catcccatgc gccgacatca ttccatccaa aatgacttct gattatatgt acattttcag
     9061 atttttccat tacaccgtaa tctatggaaa tactttttag ttttgcaaat tcatcttcca
     9121 gaactttagg aaattgtgtc gatagaatat tatcggaaag tctgttcagc gattcatgca
     9181 gctccggcat aaatctttcg atctcctgca tcattgtatc cgtcctgaaa ataaacatac
     9241 cactgttcca cagaaaatcc ccgctctcaa gaaatgcctt tgcaacttcc atatttggct
     9301 tttcagcaaa agtttttact ttgtaaattc tttcggtcga tgtatcgttc ttactttcaa
     9361 tttctacatc ttcataaata tcagaattgt actgtatata accgtaaccg gtctcaggca
     9421 aagtcggatg tataccgagt gttacgatcc cgccgttatc atttacaaat ttcaaaccac
     9481 tttttattac tctgtgaaat tcatcaacat ttccaatcag atggtctgct gctactacaa
     9541 gtacatttgc ttccggctga aatctgttta taaatacaca tgagagacca atgcacggag
     9601 cagtatttct gcctaccggc tcacagatta tattctcttc aggaattttc ggaagctgcc
     9661 gctttacttc ctgtttgtaa attacattcg ttacaacaaa aatatttgta tttttaaaaa
     9721 cgccgtcaag ccgtttgtaa gctagctgaa gaagtgtgtc atcatcatct gtgatcttaa
     9781 gaaattgttt tggaactttg gaattgcctt ttggccagaa tctggttccc acaccgcccg
     9841 ccattatcac tgcatagttt atcatttatt ttaagtttta atttttctta attttattaa
     9901 atatctttac cggatcattc agataagaaa tgaagacatc ctgatcttca acctctttgt
     9961 ctattattaa tgctttcata ttataaataa aataactgaa tatctctttg atgtcatctg
    10021 ttacagggtt tattctgtaa ctgttcagaa atttcaaaaa aatatacccg gcttctgaaa
    10081 gctgtaccat ctttgttaat ttcagatcct tagctattaa aaccgtttct ttaaggttgt
    10141 tcagatttgt tgaaagactt ctgagtgctt catagatctg aaattcacct tctattttat
    10201 acagagcttt aaaattttcg tcagtgtcct ttataagttt tttcagatta attaattttt
    10261 ccttttgatc ctcttcagga aatctttcgt ccagtttggt ttctatttcc tttttgagat
    10321 ctctctgctt tataaattct tctctctttt tattttcctc tatcagtcct tttctgatat
    10381 tctctattga cttcagaatg tttttgtatc cgaaataatc ttctcccttg ataagattgg
    10441 aaactaatat cagtccctgt ttaaataaat ttaaagtgct ttccgtaatg tcatatttcc
    10501 cttttgcaat tttttcaaaa gaaagtctta ttgcctgata aatattcgaa ataatctcaa
    10561 gtgacatttc tttggaatta agctcgagat tatcgctgat atccctgatg ttgcttataa
    10621 tattatttct ttcctcttcg ttatctattt tagctgtaat gatatagatc agactgtcaa
    10681 attctgaatt cagctttccg ttaagggatt ttatttctgt ttcaaactca atgaattctt
    10741 cattggcttt ttgctctact atatattctt ctttttcaga gtcagttgat cttacagatt
    10801 catctgagta tccggattca cctgatcctt ccttgtgtcc tgatgcactg tcaagaagtt
    10861 tttccttcag ttccttttca tacttattaa gctcttcctg aatatcactg ttgagattca
    10921 ttttttcttc ctgctctgaa ttttcgtaat ctccagcagg atcattcata ggttcctctt
    10981 tagaaatatc tgagtcatta gtatttaatt cttcagtatc ttcagtatct tcagtatctt
    11041 catccgattc ttcagaatta tttattccgg ctgcaggcag aactttttta ataataaaat
    11101 attctccact atcatcataa tttaaatctt taagtttatt aaaatttact tcatgatttc
    11161 cttcacttaa atcacctgag tttgatacat tttcatcttc ttcctgagtt gaattttttc
    11221 cggatgtatt ctcctcactt tttgattgta aattttcatc aacacttaaa ctttctttta
    11281 cattatcatt tacagctata gtattagcgt taacattagt tttatcaact tctgattcag
    11341 ttaaatcatt ttcattcaaa tcaggcattt caggaagatc cgattcagga tcttcttcca
    11401 ccataccctc agcgcttgtc tgctcttcat tttccagacc cggaagtata aaagccacgt
    11461 tatcattttc ctcttccgac tgtataagct ccatataatt ctgcttaaac gtcatcatct
    11521 cttcgtcatc ttcttcatca acaatttctg taagctgatc ctgttttatt aattcagatt
    11581 ctacatcgat aatttcattt ccggaattgt cttcttctgt aattccattc agatcaaaaa
    11641 cttttttgtt gtttactttt actgactcac ttctctcacc agataaatat tttaaaaact
    11701 ctttgtgaat aaactctgaa tcctcttttg cattctgagt caggttatca aatgtaatca
    11761 gattatcgtc aagttttttt aatatataga tcagatactt tcccgtattt ctgaattcct
    11821 taatatgata aaggatataa agatcacgta aaagattttc tgatctgaga agtttgtcaa
    11881 taatttcatt tatattttga aactgtttct caccgacatt atactttccc agatcgttga
    11941 tcgatttttt gaaaataaaa tttatgtaat ctttatttct actttcagac atttgattga
    12001 ttttaaatat aaatcctttt taccctggat gaaacgttgc acgtgatctc atatgaaatt
    12061 gtttctgcta atgctgcaag tttataagcc ggataatttc ggccaaagat ctcgacatcg
    12121 tcatttacat taatttcatt tttcattcca atatctgcca ttatccagtc catacaaacc
    12181 gttcctgtga cattgtataa tttacctttg ataaatattt ttgccttatt gctgaaagat
    12241 ctgatatacc cgtcaccgta tccgattggt actgatgcaa ttcgggtttt gttttttgtg
    12301 aaatatgttc tgccgtatga aatactttca cctttttcga gttgtttgat aaattttacc
    12361 ttagacttta atgtcattac aggttctatt cctatgccgt tttttacttt tttttcgtca
    12421 ggataaaatc cgtaaagtga gataccgggt cttaccatat taaaatatcc gtcattaaag
    12481 ttcagaatcc cgccggtatt gctgatgtgc cttagttcaa attttataat actttcttct
    12541 aaggatttaa ctaattttct aaattcagct tcctgttttt ttgcaaacaa atttccggga
    12601 atttcacttg tagcaaaatg agaataaatt cctttcagct tcagattttt cattgatgta
    12661 atttctttga cagcatcata agcatcattc atccgaaatc cggttctgtt tatgcctgaa
    12721 tccacctgta tgtgaataga aattttagtg ttaagtttag cagctgcttt gttaagtatt
    12781 actgcagacg atgtgtcagc aactgaaaac tctacgtttt tacaggctaa gtcttctaaa
    12841 agtttattct tctcagttat aataccgaga cacaataccg gagttttata taattttttc
    12901 gaatccagat aattccttag ctcagaagtt tcagtataat cagcagttcc aagataatca
    12961 gtacctgatc caataagtga ttcagccatc acattcattc cgtgaccgta tgcattagct
    13021 tttactatcg aacaaatttt tgtatcagca ttttttctgc aggcgtattt tcttactttt
    13081 aaaaaatttt tattgaaaat tttaagatct atttcagcga aggtatcact gaagtttttt
    13141 tctgcggtct tcaaattcat gcaataatgt aaaaaatcta attgctaaaa agaatttatt
    13201 tatttgtcta ttcattaatt cattaattat tttttgattt tttctaaatt aatttcaaat
    13261 caaacttgaa aattataagc agtttgttta atattaacta aggaaaaaag aaacagaaat
    13321 cctgttaata tattgttaat aacttttaac ggacagggtt ttaaagtttg ctggcatgcc
    13381 ccactaacta cttactaaca tcaattgtta ataagactaa actaactgca taacggctat
    13441 atatacaaag gcgttaacac aacatgtttt tttaataagt gtgttaacat ttatgatttt
    13501 cctgttttat tttattgctg cacataaagt aaaatattaa ccaaaattca tttgttaata
    13561 atttttaata ttcttaggtg tgataaattt ctctactgct gattcccaaa aagacaacga
    13621 taaaaacaaa aattccattt ccggcaatga agagctgaaa ctcagcgaag agagtcgtca
    13681 gaccgaactt atatggaaaa aattccttga actgctatct gataatctca aggcgggtga
    13741 gataaataca tggttctccg tgatcattcc gaaaagttac aaagataacg tacttacaat
    13801 ttctgtcccg agcgaagatt actacagtct catcgaaagc cgctataata aaattatttc
    13861 atctatcact gaaactcttc ttggttcaga cggcaaactt aattatgaaa tatcacaata
    13921 cggattattt ggtgacaatg aatcagaaaa catagctaaa aaaaatcaga tcttagaaaa
    13981 aaagaaaccg gcttttggcg aaattaatat ttttgattcc ggggttagtt caaattatta
    14041 ttcaaatgac aattcgaatg ttaattcaga ttcgcataaa aatttctcaa ccaatttaaa
    14101 taaaaagcat atatttgaaa attttataaa gggtgaaagt aatgagctcg ctgtagctgc
    14161 tgcttatgct atttcaaata atccgggaaa gacatacaac ccattctttg tttatggcgg
    14221 agtcggactt ggaaagacac atcttattca ggcgattgga aatgagattc tgaaaaagaa
    14281 tcctgatatg aaagtttatt atacaacggc accggatttt acaactgagt ttacaactaa
    14341 catcgctcaa aacaggatag atttttcttc aaacagaaac ggtacaaaga aattagattc
    14401 gttttataaa tccctagatg tacttatact tgatgacgta cagtatctga gcggaaaaga
    14461 aaagactcag gattttatgt atcagatttt taatactttg tataatgaca aaaagcatat
    14521 aattttttca agtgacaaac ctattaatca gatcaaaggt gtagaagaaa gactgatgtc
    14581 gagatttcag tggggaataa caactgatat acaagctcca aactgggaaa tgagagttgc
    14641 tatcattcag aaaaaattaa tggaagctga cattgaagca cccgaagaag tggttcattt
    14701 tattgcttca aatgttaaag acagtatcag aaccattgaa ggctgtatag tcggaatcat
    14761 tgctgacagc gtcctgagat acaaaggcga gatcaatctt gagattgccg aaaacgttat
    14821 tagcagagtc ataggcaccg taaagcgcgc taagaacatt tccatcgaaa acattatata
    14881 cgcagttgca aattattaca ggatctcaga aaatcagata ttatcaagaa agcgtacgaa
    14941 agaaattgcc tttgcaagac aggtagcaat gcatcttgct aaagaactta caccgcttac
    15001 acttgaatcc atcggactta attttggcgg aaaagatcat gcaaccgtcc tttacgctaa
    15061 caatattatt tccaatctca ttaaaaaaga taaggacgta cttaatcaga tattagaaat
    15121 caaagaccat ttaaaaaatt tataataaca attctctttt tcttttctta agataataat
    15181 gccacaaaaa aaagcttgca agcgatctgt aaggtttcca tttttctgaa aatttaacaa
    15241 cctctatttt tgttttacag cccgttaatt caatcgcagc gtttataaca gcaacatctc
    15301 ccgcagggaa gatatcttta gcctgaaggg caaacatcag ataaatatca gccgtccagt
    15361 caccgatccc tttaattttt tttagaattt ctcttgtttt attttgatcc tgacctgata
    15421 aattttccag atcaagttca ttatttaaaa ttgcatttga aagtgaacga agatattctg
    15481 atttctgtct ggttatataa caatttctca tttcagtatc tgaaagcttt ataatctgag
    15541 ccggtgtgaa ctcctgaacg aaatctgaca gtttcaaaaa atgtgaattt gcagattcca
    15601 gacttacctg ctgaccgatg atgatctttg acaatgtaat aaatccctgc ggtctgatca
    15661 cgttttcagg tgatccgtaa gcatcatgaa tttggctgaa tattttatct tttttaaaaa
    15721 gattatctat gtctttgtta taaataattt tatctttttt attaaatacc atcggagaat
    15781 ttttttttga aatataattt atattctttt aattaaataa ccgattcgga taagtagaaa
    15841 cttctatatg taacccatac gcagaaacgc caatatgagt ctatatatta ttaataatcc
    15901 aaaaaactga aacctggcaa attttaaaag tagattttaa ataaagacat atattattac
    15961 aataaatacc cattgttaaa atcttattca tatcttatta ataaatcata actactgaca
    16021 attttaaaaa aattttaacc tattctatct gtaattgtta gtttttttta tttaagctat
    16081 ttttattaac aagttatcag atggtatttt gaagacaatg ctgataaact taattactaa
    16141 caatattaac atcactgcta ctgctactaa tcttttatat atataattaa taatgataga
    16201 gaaattactt attaagaact acctgataat aaaagaagct gaaattcatt tctctgaagg
    16261 cttaaatatt cttacaggcg agaccggtgc agggaaatcg atcattcttg atgctctgtc
    16321 tttactgctt ggtgaaagag ctgatcattc caaaataaaa agcggagatg ataagcttat
    16381 tatggaaggt cagtttaatt ttactgataa taagatagtt gaaaatcttt ataaagaaat
    16441 gtttccggag gatgagttta atggctatct gattctcaga agagagataa atacaaaggg
    16501 aattaacaga agttttataa atgacacgcc tgtgaatatt tctgatatga aaaaattcgg
    16561 tgatgtagta atagatattc attcacagaa tgaacatcag tctcttctta gtaaggaaac
    16621 tcatattgga attcttgata attatgtatc tgatgaaact attttttccg aatatgaaaa
    16681 aggttatttg gatctgagaa aaagtgtttc ggattataaa gaattcattt caaaaaaaga
    16741 agatctgctc agccgaaaga aatttttaga ccatgatctt aaggaaatta ataatgttaa
    16801 tatacagccc ggagaagatg aagaaataga gactgaactg gacagacttg aaaattcaga
    16861 ggaaatatca tcatctctaa gtatttctct tgaaggactt tctgaaaatg atttcaacgc
    16921 tctaaatatc atcagtgcag cagtcaagga attaaagaag atatctgatt atgacaagaa
    16981 ttttgaaaag cttatagagg atctggaaag ttcatatata ttgataaaag aaacttcatc
    17041 ttcattatta aattataata atgatttgaa ctttgatcct gtcaggatcg agcatttaag
    17101 aagcaggtta cttgctataa acagtttaaa aaggaaacat gagcttgata ttccggaatt
    17161 acttgaaaaa gctgagaaca ttcaaaaaga tctgacattt gcggagaatt ttgatcatga
    17221 aattgataag cttgataaga aaatttcagt aatgaaatct gaactgttct cacttgcgga
    17281 aaaaatttca gataaaagaa ttaaaagagc taaagatctt gaaaaggaaa taaataaatt
    17341 gttaaaagaa gtcggacttg aatctgcaga atttaaagtt gatataaaaa aaaacatttc
    17401 cgattcagaa aatgaattta ctgtaaaaaa gggaaaggaa aattatgaac tttcccaaaa
    17461 cggcattaat aatattgaat ttttaataag gacaaacaaa ggaatggagc tttctccgct
    17521 cagaaaatca gcttcaggcg gtgaaatttc acggataatg ctcgccatca aaacagtgct
    17581 ttcggaaaat gacaatatcg gaatacttgt ctttgatgaa attgatgcag gtatcagtgg
    17641 gaggatagct cagaaggtcg gtaacacact taaaaaactc gcagtctcac atcagatcat
    17701 ctgcatcaca cacctccctc agatagccgc aaacagcgac agacatttta atgtaagtaa
    17761 aagtgatgaa aataatgaaa cgaaagcata tattaatatt cttgatgata atgaaaagat
    17821 aaatgaggtc gcaaaacttc tcagtggtga aaaaatcaca gaatctacta ttaacagcgc
    17881 taaagagctt atttcaggga aataaaaaac ctccacataa aatttattat gtgaaggtca
    17941 attaaaggaa gaacaaatat gaaagaacgt agcttgctgg cgcaagcagt atcgtataac
    18001 attatcaggt taataaagtt tcagaagatt aaattttttt tttaaaaata atatttttaa
    18061 atttctgtaa ggaaattagc aaattcctgt gaagttatat ttccaaggta atgcttgagg
    18121 tcaatatcag aaataccttc ttttattgaa atgttatcat attttaaacc tgtcagctta
    18181 ttttccactt cgctcaattc accatgacca agaaaatccc cgaatatttt gaattctttt
    18241 ataatcccgt cctttacttg tatccttgca tcaatctgtc caaaatcaaa tctcttcttt
    18301 ttctgtatat taaattcagg tgacctgcca taattccagt cccaagatct gtattttgat
    18361 tcggaaagtt tttttactcc ttcaagctca tcatcagaaa gagtataaaa ttttatattt
    18421 tctttacttc cgaaaataga ttctgtgata atatctttga aatcgtaaac tgacatatca
    18481 atatttataa attcttttat atttgctaca cggcttctta cggatttaat tcctttactc
    18541 tcaatcttgt ctgtttgaac gttcagagct tttactacat tatcgagatt tgaattaaac
    18601 aaaagtgttc cgtggctgaa cattgactta gtatcagtaa actgtgcatt ccctgagatt
    18661 tttctttcgc tgacagtaat atcatttctt ccgttgagct cagcatttat tcccatttcc
    18721 tgcagaacct taatcacagg ctcagtaaat aatttaaaat tatgaatact ctggtgtgaa
    18781 tgttttgtca taaagctgaa attcagattg cctttgtcat gatatacagc tcctccacca
    18841 gaaattcttc ttactatatg aactccgttt tgcttaacgt aatcattatt aacttcttct
    18901 atagtatttt gatgttttcc aattatgacc gaaggttcat ttatataaaa taaaagatat
    18961 tcattttcca tattcagatt acgtacacaa tattcttcaa gagccagatt gaaagtcgga
    19021 tctgtgatgt tttgattatc gattaaaagc atttaaagtt atttgttttc gggaataatg
    19081 gggttcgtaa gctgtgctct gcattgttca tcccattctt taatttccac accggtgact
    19141 ttattcattt caccttcact gtatctggtt ttaatttttt cgagcataca tttacagaag
    19201 gatttcataa tttcctgttt acccatgtcg tctttgattt gaggcttata cttatcgtag
    19261 caatttttta caaagacgga atccatttct gcagaccatt cgtgaaaagt cggatctttg
    19321 tgattctgac tatccatata catataaata acacctgctg ctattatacc ggcaataaga
    19381 aagagccata gcctttcctt ttttggtggg ttttttgagt cgtttatctt attcaattta
    19441 aatttatttt tttcaaatat tacaattatg ttatgatttt taaatacaga atctaatact
    19501 tcttataaaa aatatttttc caaaaaatca caatgtttta aattctccct ttttagttta
    19561 gttgaatttt tgttatttgt aaaaatcaaa aaaatatgat accacaattt aataatctcg
    19621 atcagaaaga aacagaatta atgctggacg ctcctgcact tgtaacctta cttatagcag
    19681 ggtcagacgg caatatagaa caaaagcagg tagactgggg atcaaagatc actcatttca
    19741 gagctaaaga cgaagattct ataatgcaaa gttattacat agaagtggaa aaatctttta
    19801 acgatacact tgcgcagttt attaatgcat ttccaaatga tatagaggaa agaggaaaat
    19861 taataaataa tgagcttata aaaataaatg atattcttcc aaagcttaat aaagactttg
    19921 caaaacagtt ttacagtgat atgaaaagtc tttccaaaca gattgcaaaa gctaccggcg
    19981 gtgtctgggg atacggttca ataagtgctg aagagcaaaa atatcttgat cttgatatta
    20041 ttaatcctgt tgagtaatta aatctgaatc aggatcttca atttcttcaa gccgacgtga
    20101 tcttgatatt attaatcctg ttgagtaatt aaatctgaat caggatcttc aannnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttcttca agacgacgta aaatattttc
    20281 gggcagagac ttattggttt ctgccccgag ttttttaatt ctctctattc ttcctattat
    20341 tgtgtcacct ttcttttttg attcaaaaag tttgttcata gcactgctgt aattttcttt
    20401 tgaatcgtcg attttatttc caagcgtaac gagatcattt ataaacccgt gaaatttatc
    20461 atacaaagct ccgctctcgt ttgcaatttc aatcacattt tttctctgtc tttcctgttt
    20521 ccatataaat gaaattgttt tcatggtagc gagaagtgtc gaagtggaaa cgggcagcac
    20581 atttttttca agagctcttt ctatcatttc tgtgttttct ttcagcacgg aatgaagagc
    20641 aggctcaata ggtacaaaca tgattacgta atcaggcgga tttattccgt aaagttttgg
    20701 ataattcttt gaactcaaat ctgatatgtg attcattata ttttttaaat gctctttgag
    20761 aaatgaagct ttgtcattat cattttcaga attaaaataa ttttcgtatg cgacaagaga
    20821 aaccttggaa tctatgatca gatttctgtc ttcaggtaag ttgataatat aatcgggtct
    20881 gaaaacattt cctcctgcat caagaagtgt ttcttgtttt ctgtaatgta tatctttttc
    20941 gagaccggct ttttcgagaa tcagctcaag ctgcagttca ccccagttac cctgaagttt
    21001 tttgtcacct ttaagcgcac cggtcagtct gtttgtatct tcacttacct gctgatttag
    21061 ctttacgatc tgttcaagtt cttttttcag agaaattctt tcacgcgtct cttctttaaa
    21121 tgtatcgtca accttttttt caaaatccga tatcttttct tttaagggtt ttagaatatc
    21181 accaatgttc ttttcattaa gctcagtaaa ttttttccct ttctcttcaa gcagttcatt
    21241 agaaagattt ttgaattcgg ttttaaaaat gttgtgcaga tcttccatat cagatttctg
    21301 aacttcaagt ttttctgtta aattttttga atgttcttcg gtgcgggcaa gagatctgtt
    21361 caattctaaa atttcagatt cctttatagc taaattcttt ttttcatttt cataaaggtc
    21421 tttcggaatg taatttgatt cgatctcttt aagtgataat cctttatcct tttctgaaat
    21481 gagtttaatt attatccatg cgatgaagaa tcctgatatc agcgcgatca ccaaataaag
    21541 tatattgcct gatgactcca tatttgtttg attaatttaa tgagtctgat aatttacaaa
    21601 ttcagtattg taaaataaat attaatctga tttgaatttt ttgaaaaagt aatttagtca
    21661 aaattagctg atggagaatt taaaaaactc aataaaaaat tttactttgt aactacattt
    21721 aaaaataaag atattaatac tcattacaac tgaaagaccg gttaaattaa ttattgaata
    21781 aaaatttaaa atatttttac ggtatatttt ccctgctgat acttactttg atctgcagca
    21841 tcatctttat gaaatgcact gatgatagtg atgacagaga taaagtatta tcaaaacttc
    21901 atgagaaata ttattatctg aatctatatg aagatgtaaa atatacaggt acagaaaaat
    21961 gtgtctcatg tcacaatgac attggtgaga cttttcatca cacgggaatg ggtagttcac
    22021 tttataaacc tgaagcagga aatgaaattg aaatttacga aaaggataat attatctatg
    22081 acgggaaaaa tgatctgtat tacgaagctt acagaaagga tggaaattat tttcagagag
    22141 aattcagaaa agatgagaat ggaaatctca ttcactctct tgaaaaaaaa gtagattata
    22201 taataggttc cggatttaac acccgcagct atttgtatca ggataatgga ttcttttatg
    22261 agatgccgat gtcatggttt acggaaaaat caaaatggga tctgagccct ggctatcaga
    22321 gactgaatct cagattccga agagagatca atcaggaatg tatgaattgt cataattcat
    22381 attcgggata ttttgaattt tcagaaaata aatttaaaat gccaatagct gaaggaattg
    22441 gatgcgaaag atgtcatgga ccgggtgaac ttcatgtcag aagatttact gataaggata
    22501 atttttttac ggctatagaa agtgacacta ttgacaggac aattgtaaat cctgcagatc
    22561 tgccgcttga agaaaaactt tctgtttgtt ttcaatgtca tctgcaggga gatgttaggg
    22621 tttttactga cgggaaaaag cagtcggatt tcagacctgg tatgaagctg tctgatgtga
    22681 agacagtttt tgttcaggat gatgtttccg aaggagattt taagatcgca tcgcatgcag
    22741 agcgtatgtt tctcagcagt tgttttaaaa attccgacgg agggatgaca tgtcttacct
    22801 gtcacgatcc tcatgttaca gttggaaatt caacaaaaga attttttaac aataaatgtc
    22861 ttggctgtca tcaacagaat tcattgtctc cttcaaagac aaacgtcgac cataaagcag
    22921 gcagtaactg tatcagttgt cacatgaaac aggggaatac ttcggatata cttcatgtaa
    22981 actttactga tcactggatc agaaaaaaga caggtgtaga atcagataaa atatccggtg
    23041 atgaaaattc acaggcagat aagagtgtca ttcctgtcac tctaaaaaat tttgaattta
    23101 cggtcaatga aaaaaataaa aattcaggtc tggaactcgg gattgcatat gtaatttatt
    23161 atgaaacgaa acatccgcat caggaatatc ttaagcgggc acagccttta ctggaggcgg
    23221 gagtaaagac agatccggaa aacaggtcag cgctgtatta tcttggatca gcatattatc
    23281 tcgataacaa atattctgaa tctgtaaata ttctcgagtt acttattgca aaagatacag
    23341 gaaatgccga agcttttatc agactcggag cagcatatga aaagtccggt aatacacaaa
    23401 aggcaattga gtcttatgaa aaagcattga atattttccc tgagaatgta aaacttttaa
    23461 acagtctcgg taatgtttat tattcatctg gtaagataaa tgaagctgtg gagacttaca
    23521 aaagaggtat aaaaataatt aaggataatc cgatcattct cacaaatctc ggtgacataa
    23581 gtttatataa actggaaaat cctgatgctg ctttcaatta tctaaacaag gcactcgaat
    23641 atgatcctga taaaaaggaa actctgaaca atctcggtaa tgcatatctg ataacgggaa
    23701 ataaaaatga agctgaaaag atatttaaaa gagtattaaa gctcgatgca aataacgcac
    23761 tggcacttgg aaatcttgca gtcatttatg aagacaacgg aaataaaaac gaagctgtga
    23821 agatgttaaa gaaaatactt gagaatgatc ctaatgataa aagagctaag gaaatgcttt
    23881 ctaagcttaa ggggaaataa atattttatc ttaattcact cagaatccga agatcacttg
    23941 acattacaga aatcacagga aaaaaatacc gcaaataaaa ttaattattt gcgggataat
    24001 ttaaatatct gattaagtat ttgtaatttt aaatctataa attgaaattt atttagtcag
    24061 gatcatcttt tttgttgaga cgaaattacc tgcgttgatc gtatagaaat aaactcccga
    24121 tgcgagtcca tctgctttga actcagctga atagcttcct ttctgttgaa cttcatttac
    24181 aagtgttcgg acctgtctgc caagttcatc agttacagta atttttacaa actcctgatt
    24241 cggcagatag taagtaatgt ttgtcagcgg attaaaaggg tttggataat tttgtgacaa
    24301 ggtataattg tctgcaattt cagtactgtt attatttatt gaagttgtat ttcctacttt
    24361 aagaacgtac agaccggtag agatatcact agcgatgata tttcccgatg gcagataagg
    24421 atagacattc cagcagccct gatatgcagt actgttagtg ccgggataag tatcatagtg
    24481 accgacctca acaggcagag aaggatttga aatatcataa actattacac ctgctctata
    24541 atgtgaaaga taaagtgagt cgccttttat gtaggcattg tgaaccattg acgcactgtc
    24601 ataaggaatg atctctgata atctgacctg atttgaaaaa tccgaaatgt cccagagaat
    24661 tgcatgattt gtgccacctt catcagtggt caccagaatg tttctatcat cagtggtcca
    24721 tgcgttatga gtaacagcag aagggtaagt gaattcgcgg ataaatttta ttgagtcctt
    24781 atttgtcgca tcaagaaaaa ccataaatcc ctgattaata gaagctgcat agatagtatc
    24841 attctttata aagcaatcat gtacatatcg ggtaccccat tgtcctctct ttacaggatt
    24901 ttccggatcg aggagatcaa gaataaagac accgccgtta ttataatttc cgccgttgct
    24961 gtacaggaat cttccgtcct gagagattga gtgggatcgg gtataaccgg tgaatgtgta
    25021 attttttaca aaatgaattg aatccggaag atactgcaga tccacgatct gaacacctga
    25081 tccgccttca gatacgatat ataagtaatg cgaataagtt ttcatttctc tgtaattata
    25141 aaaagagcct ggtcctgcga ccgtaccttt gttaattata ttatcaggat tagtaacgtc
    25201 atagacggta gtaccggcat aatgaccgat gatcgcatat tctctgccat ttgctacata
    25261 tccccagcaa gctgcatagt gccagccgcc aggagttccg cctgctgtat attcatttgc
    25321 tgatccaagc atctgcatat tgcttacaga ctgagaaaga gaaattccat aaaatgcaaa
    25381 cagcattata agtgttaata ttttattcat agaaattaaa ttttaataat aattttacaa
    25441 taatatatat ttaatgaaat ttaaaaaaaa cattaattac aaatttatat taaatctcag
    25501 ttttaaaaat cttagttaat tcagatgtgt agtttttgaa ataaaacata ttgattttga
    25561 tgtttcgaaa gaattaaaaa accgaatatt atatttagca taaaatatgt attttagaaa
    25621 actaactaaa atttaacgag aagaaatagt taaaaaagga caaaaattat ataaaatgaa
    25681 tactgcaagc acaatagtcc aaaaaattgt aattactttg agaaaagaaa ttacaaacgg
    25741 tattttaaaa tccggcgcac atttaacaga aacgtcggta tctaaaaatt tcaacgtcag
    25801 ccgcgtacct gtcagagaag catttcgaat actacaaagt gaaggataca tagatctgat
    25861 ccataacaga gggggttttg taaaaagtgt ttcacgtgat aatgcaattg agaatggaga
    25921 gatatatgta ctgcttgtac ctatgatcct taagaaagca attccaaatt atacaaaatc
    25981 aacttttaaa aaagctcatg caattcttga taaaattgag aactgcgaaa aatactgtga
    26041 ttccagttat ctcgtatggg attttgctaa agtaatttat tctccttcaa aaatggaact
    26101 ttccatgaaa ataattaacg acatttacag tcagggtatc agagcactga atgattattt
    26161 tgaaattgaa gacactacaa aagttacaat tgatagtcac agagaatttg taaatctttg
    26221 cgaaataaaa aagtttgatg aagctgctga actttggatt aatcatgtag aacacgtaaa
    26281 ggaaaggatt ataaatagtt tacctgactg acctgttata aatcttcctg tataagttta
    26341 atatagaaga ataaaatatt agaatagttt atcaacagcg tcatttattt tttcaactcc
    26401 tgttacttca gcattaaatt tctttgagtt aatatttttc agattacctt ttggaattat
    26461 tattctttta aatccaagct tagctgcttc agtgattctt ctttcagcat aagaaattgt
    26521 cctgacctct cctgataatc caatctcacc gagtaataca gtttcagaat ccacaggaat
    26581 atcgcggaat gaagaataga tgctcattgc aactgcaaga tccgttgccg gctcttcgat
    26641 cttaatacct cctgcaatat ttaaaaatat gtcagactta tttaaaaata tcccgagttt
    26701 tttttcaagc acggcaacaa ttatatttag ttttttataa tcgaagccca tggaagtacg
    26761 ctgtggaatg ccatagttag aagaggttac aagtgcctga acttc
//
LOCUS       DAORVQ010000022        26701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_644_length_26701_cov_5.749456, whole genome shotgun sequence.
ACCESSION   DAORVQ010000022 DAORVQ010000000
VERSION     DAORVQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 26701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26701
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_26701_cov_5.749456"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..2873)
                     /gene="mutS"
                     /locus_tag="PLX80_03395"
     CDS             complement(258..2873)
                     /gene="mutS"
                     /locus_tag="PLX80_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQY19872.1"
                     /translation="MKNETPLMRQYRKIKEKYPDTLLLFRMGDFFETFENDAVTASKV
                     LGITLTKRSNGAASEVPLAGFPHHSLDNYLPKLVKAGFRVAVCEQLEDPKYAKGIVKR
                     DVVEVVTPGANFSEKLLDHKSNNFLAAVYIKDNVCGFSFCDVSTGEFATSEILTKNLC
                     EQIENINPTEILIPKKDKEKIYKLLDYDPDAAFSEQRNKHFFTKIDDWVFNSDYAKEV
                     LTNHFGTQSLKGFGIEDMNEATVAAGCVLNYLNETQKNNLPHIKKIYNYEFSDYIILD
                     PSTKRNLEITSSIAEGGREGTLISILDRTKTAMGGRLLKKWVSRPLKKKEQIEKRLFA
                     VKDFFDNKNERKKISEELKSVADLERLLSKISTGRAVARDIVQLKISLKKINIIKAEL
                     LKFPSTAVQAIGTNLIEINELILKLENCINENFVSGIDNYGMINKGYDKDLDDIKDIL
                     INGKTWIENYQSKERAATGINSLKVDFNKVFGYYIIVTKTNLEKVPSNYVRKQTLVNA
                     ERYITDELKVYEEKILNAEEKIEELEKNIFNELKNFVLDFTDQVQRNASLTATLDTLI
                     SFAEVSEDYNYVLPEVSDNDDLEIIDGRHPVIERLLPPGEKFISNDTFADIKNSQILI
                     LTGPNMSGKSSYLRQVGLIILLTQIGCFVPARSAKIGIVDKIFTRVGASDNIAKGEST
                     FLVEMHEAANILNNATSKSLILLDEIGRGTSTYDGISIAWAMTEYIHENPNINAKTLF
                     ATHYHELNALADMYPRIKNFRVEVKEYNDKVIFLRKITEGTADHSYGIQVAQMAGVPE
                     KVTKRAKEILRSLEDKEYRRKTKDDLQISMFESAVTEIKDEALRKMISDIEIDNITPL
                     DAMNILKKLKDETDN"
     gene            complement(3029..3613)
                     /locus_tag="PLX80_03400"
     CDS             complement(3029..3613)
                     /locus_tag="PLX80_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY19873.1"
                     /translation="MSKSKAEDMVLIDEALAGRQNSYAKLMKKYQPMITHLIYRMISK
                     KEDVEDLTQEAFIKAFNSLANFDKQYAFSTWLFKIATNNAIDYLRKKKLYTFSIDKKI
                     QADDSDYSFEIPDHGNKPDNNVIDKQLRIILDEAIESLPSKYKDVIVLRHKKEKEYEE
                     IAKELNLPLGTVKAHIFRGRELLNKYLKDKIKHY"
     gene            3788..4888
                     /gene="rlmN"
                     /locus_tag="PLX80_03405"
     CDS             3788..4888
                     /gene="rlmN"
                     /locus_tag="PLX80_03405"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQY19874.1"
                     /translation="MKNILNFDLAALEKEITESGEPKYRAKQIFTSLHKNNVKNINEI
                     KGIPKYLKNKLEDLYEIPELKLLEVRNSEQFNTKKFLFEINDENKNHIFESVLISEKG
                     RHTICISTQIGCNVGCEFCATAKMGFLKNLDTSEIIMQVYEIIRNTGIIPTNLVFMGM
                     GEPFLNYNNLLNSLRILTDPEGLSIPSKKITVSTVGFKEKIKKFADDISLEENKNIRK
                     VKLALSLHTTDNGLRESIIPTSAKNRLSDLYEELSYFYRATGSKITYEYIYFQGLNDT
                     DNDIKRLTKLLRMVPSNLNIIPFHPIDFKLKAPLDIFDKDKDLNNLLLNKNLFDFIEK
                     LKLNKVTVNLRSSSGVDINAACGQLAVIKSQS"
     gene            4919..5467
                     /locus_tag="PLX80_03410"
     CDS             4919..5467
                     /locus_tag="PLX80_03410"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQY19875.1"
                     /translation="MFQLIIFGPPGVGKGTQAASVAEKLNLAHISTGEILRKAVSEET
                     ELGIKAKEIMDKGNLVPDEIMNGIVRETLEKVNGNGFILDGYPRTVNQAVALAEIFEK
                     LKFYDIKVITLTADDNEITERLLKRGRSDDTRDTILNRLKVYYNSTSPVKEYYEKQGI
                     NMDVNGVGDIKEINESILKSVN"
     gene            5574..5921
                     /locus_tag="PLX80_03415"
     CDS             5574..5921
                     /locus_tag="PLX80_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY19876.1"
                     /translation="MASIKETELSDKGIVILSPKGNFVGGDETDELREAIKKYTDLGN
                     KKLLIDLGEVLYLNSTALGVLIAAHANYSKREGKIKLCQLNKNLENLFVITKLALIFD
                     SHTTQEEALNSFE"
     gene            5985..6770
                     /locus_tag="PLX80_03420"
     CDS             5985..6770
                     /locus_tag="PLX80_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY19877.1"
                     /translation="MKQSVFMSVVLITSIVVSAAIFYGVFGSPSNFKTPEMHTPSNIM
                     GAIYQGGPVVIVLMALSIMVITFTIERQLSLSKAKGKGPIEPFLKKVQTSLLEGNIEE
                     AIAECDKQKGSIANILRQGLDKYQMLSMDNKVESDTKIQEVQSAIEEAMMLEVPLLER
                     NLVVLSTIASVSVLVGLFGTVIGMIRAFQALAAAGTPNAAELSSGISEALINTAGGLF
                     GAIVGTISYNYFVTKVGNITYMIDEATYNMLQILTIKDSKRSV"
     gene            6805..7395
                     /locus_tag="PLX80_03425"
     CDS             6805..7395
                     /locus_tag="PLX80_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY19878.1"
                     /translation="MKKARSIKKPGVNIDMTPLVDVIMLLLTFFMLVATFKAESTEAI
                     EVTLPESVVTDTTKLPEKDIMTLTLTKAGDVFVDVDSYKVKEEVFGSSIGIGLYHPDS
                     TSKSEYELTGGSINGKPFKRPVTMLNKEEFEKTLNDLRMTLKSQNGMSSDFRIVLKGD
                     METNFGVVEDLMESFKTTRNTRFALVTDVKSDQPDN"
     gene            7423..7950
                     /locus_tag="PLX80_03430"
     CDS             7423..7950
                     /locus_tag="PLX80_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY19879.1"
                     /translation="MAGGGFDAPDSGGGLRKQKKYKKGKKIGVRIDMTPMVDVIMLLL
                     TFFILTTTLNTPQIMQINLPKGDQEDKIKVDMGNVLYVRVSENANAYFSSGLSDGNEA
                     EPVKVSFNDMKNKIEDLYRQNPQLIILLKFDRNMKYSTMIDILDEINKAEIDKRYSFM
                     KMEDPDKEIVTKAGG"
     gene            8044..8850
                     /locus_tag="PLX80_03435"
     CDS             8044..8850
                     /locus_tag="PLX80_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQY19880.1"
                     /translation="MKKPFFKYLFLLLVFLTLYPELYSQGQKERSLADNPIQLKEYLV
                     DETGILTPVQHKDILVKLVNEDKTTSNQIVVYIINSLNGESLEDVSINLAEKNKIGKK
                     DRNNGVLMLVVRSDRKIRIEVGYGLEGALTDAISSSIIRNVITPEFKKGDYYKGINDG
                     VDAIIAAIKGEYTAEENELISQDSSKCCFGVPIFVMMIFFFIFISIFTSVLRRMFGFG
                     NSVRTGKKDSGSSWWSGGGGSGGGWSSGGSSGGFSGGGGSFGGGGASGSW"
     gene            8884..9342
                     /locus_tag="PLX80_03440"
     CDS             8884..9342
                     /locus_tag="PLX80_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQY19881.1"
                     /translation="MPKNYINKYLSDSDLDEIVKNISQAESKTSGEIRLSIKTNRGYR
                     EKGITPRELAIAEFHNLDMHKTKDKTGVLFFILFDEHKFEIIADEGINSKIEDSEWKT
                     FSEKTADLFREEKYKEGIKFVIDKIGERLAAEFPVQNDDKNELSNEVIIS"
     gene            9406..9951
                     /locus_tag="PLX80_03445"
     CDS             9406..9951
                     /locus_tag="PLX80_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQY19882.1"
                     /translation="MNDSEILLRKYIKNENLIKHCYAVESAMKYYAGLLGEDSEKWGA
                     AGLLHDLDWEMYPDTHPNTAVPILEEAGYDKEFIDAILGHAYPDRTDVPRETQLAKYL
                     FACDEITGFIMAYSLMKQNGLIDVESKSIKKKMKDKAFARNVNREDIVKGAEDIGIEL
                     DVHVNNIIESMKQDNRINNRN"
     gene            9955..>10595
                     /locus_tag="PLX80_03450"
     CDS             9955..>10595
                     /locus_tag="PLX80_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY19883.1"
                     /translation="MNIINTKDLSKEYSSKSFSKDKILALNNFSFEVEAGEIFGILGP
                     NGAGKTTLIKILLGITHRTKGEINIFGKEINDESYKLKVGYLPENHKFPNYLTGEEVL
                     TFYGKMSGLNKTEVKKRSDEFLKLVDMEKWRKTKIKKYSKGMLQRLGLAQALINDPEL
                     IFLDEPTDGVDPIGRKEIRDVLESLKERGKTIFLNSHLLSEVEMICDRVAIMN"
     assembly_gap    10596..10695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10738..11550
                     /locus_tag="PLX80_03455"
     CDS             10738..11550
                     /locus_tag="PLX80_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY19884.1"
                     /translation="MIFSTLVLSTFREAMAKKIFLGFFAISTIIILIFLFLINVDSVE
                     GMVDMVGASGEEGIRKLVIGFEVAMIDISYLLIITFCLISVSSFIPSMLEKGNIDLLL
                     SKPISRTNIILAKFIGGVLLVFISLIYLIGFVWIILSSKSGFWHFPFLYSILWLTLSF
                     AVIYSLIIFIGLISQSTILSILVSFFLVFIVCPILYVREATIFSLVENSAAQFVINFF
                     YYILPKPSDINKITTDIITGEAVNSYMPVFSSVLFMLAVLSASIFYFKKKDY"
     gene            11569..12624
                     /locus_tag="PLX80_03460"
     CDS             11569..12624
                     /locus_tag="PLX80_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19885.1"
                     /translation="MKFVKTFPVLFIIVTILIQGCDFKPEPEPLPPMVRATLNLLQED
                     PQFVMYLNFKTMSRTNFWKNNISDSLLNEEKTFGSVLNTFKIATGASVSDGLDEMYYS
                     NSWFGENSIVLKGLFNKKKLDEYLLTDSIFSITKTSSGKDIYIKNDNGLYFFFKNEFT
                     ICASNYLKQIDIMMQVSDTVNSGLMKNEKLLGSIENIIYKQNLWMVSTEKAFIKGIYQ
                     NFLGSTAGRKLEEDTTAVMKDSVKANDEKADLDNLYTKYNSISFSADMKDDIEILIQN
                     NFIDDPSAKFFKSILNGFLTVSKLSSGGDKKPPASKLLDKIKLSRYDNEVFIKIIMEN
                     KDLEQLRNSDLMSEPGF"
     gene            13200..14828
                     /locus_tag="PLX80_03465"
     CDS             13200..14828
                     /locus_tag="PLX80_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY19886.1"
                     /translation="MKIIFVLTNLYIVLNCSELLCQVNEDWTSIYNSSGTYESGISVK
                     TDNSGNVYTAGTIQTSTGFDCILIKYNNYGNEIWTSIYDNPLSNLNDGVTSMVLDGRG
                     NIFITGTTFSFENDVDYLTIKYDSSGDMKWVRTFGDTIIWGNDTTTFLSDYTEDIEVD
                     NFGNVYVTGDDGTIKYDSLGNLLWIVKDDITIQDMTIDVNSNIYITGFRYDQNTHRDC
                     RTIKYNSLGVRIWISDYMGPTYSDDQGLAVTADDLGNVYVTGFTHSNNYSDYSKYLTL
                     KYDESGIQQWAAIYGEQYERNIANDIEVDKSGNVYVTGNAFSSNLTSNDCYGTIKYSP
                     SGLQLWLTLHTGSSTARARDLELDNQGNIYVTGYFGSSIGDDYSTVKYNSNGLKIWQV
                     RFNGSFISSEELRSIAIDNVGNVYVTGDAFPNLNMDIATAKFSQTITGISEGQGLLLS
                     SFSLSQNYPNPFNPMTVIRYSIPSNFRGQVSYVKLVIFNTLGMEIATLVNEIQNTGEY
                     EVEFDGSSLTSGVYFYIIEVDSRVIDTRRMVLLK"
     gene            complement(14983..15999)
                     /gene="fbp"
                     /locus_tag="PLX80_03470"
     CDS             complement(14983..15999)
                     /gene="fbp"
                     /locus_tag="PLX80_03470"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQY19887.1"
                     /translation="MVSKFNTFYRHIIEEERNFPDASGQLSDLLADIALACKVISLEV
                     NRAGLIDILGFTGDENVQGEEVKKLDVFANDVLTNVMKQGGHICAVCSEEEENFIPIN
                     KNFGSDNSVSNKYICHFDPLDGSSNIDVNVSIGTIFSIYRRVTESGPGALEDCLQKGD
                     VQVAAGYVIYGSSTVLTYTAGKGVHLFTLDPSVGEFLLSTENISIPKKSKIYSVNEGN
                     YCKWSDGMKKYINHLKEDDESTGRPYTSRYVGSLVADFHRNLIYGGIFIYPADLKNKN
                     GKLRLMYEANPLSFIVEQAGGRSSDGKNRILDLIPESLHQRTPLYIGSEEDVLLAEKY
                     LSEE"
     gene            complement(16007..17350)
                     /locus_tag="PLX80_03475"
     CDS             complement(16007..17350)
                     /locus_tag="PLX80_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY19888.1"
                     /translation="MEIKQFYDKSLAHASYAILSENEIALIDPARDPGIYFDFADDNN
                     AEIKAIIETHPHADFVSSHLEISKKTGAPIYISKLVNALYDHITFDEGDELSLGIIKL
                     FPINTPGHSPDSISIIIKDEDGKDHAIATGDTLFVGDVGRPDLRESAGSINKTKDELA
                     RMMYNTINEKLLTLNDDVLVYPAHGAGSLCGKSTSTENSSTIGKERKSNYALQPMSED
                     KFVDELLNEQSYIPKYFGYDVELNRKGAPDFEESVNNVKIISSYNDIEDGHIIIDTRS
                     KEDFENGHYPGAVNLREDTKFETYLGTVVSPDEKFYLISDSKEKLDTLIRRCAKIGYE
                     LIIEGALVHEEIEDSVKVEKLDLNDFKENLSEFTIVDVRNLSERNSEIIFEDSIHIPL
                     NEIRERYNEIPSDKPVVVHCAAGYRSAAASSILRDKLDTKVFDLSDEIKNFSKYN"
     gene            complement(17359..17679)
                     /locus_tag="PLX80_03480"
     CDS             complement(17359..17679)
                     /locus_tag="PLX80_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY19889.1"
                     /translation="MQHKDIDTKSFKELSENDAYVLLDVRTPDEFNSERLKDSINIDF
                     YESDFEDKLDELDKRKKYLIYCRSGNRSRQAMFLMRDLGFEEVNNLSEGIISWVENNF
                     ETIK"
     gene            complement(17698..18510)
                     /locus_tag="PLX80_03485"
     CDS             complement(17698..18510)
                     /locus_tag="PLX80_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY19890.1"
                     /translation="MSEIYGYLAAVFVGLVLGMIGAGGSILTVPILVYLMGINPVLAT
                     VYSLFVVGFTTLIGSVSYIRQGLVNIKTSIAFAVPSLVSIFLTRKYILPSLPEVIYQS
                     GDLIISKDIFILLLFAVIMLFSSLSMIFSNVKEKDDHHSMTMNENLILVFYGFAVGFV
                     TGMVGAGGGFLIVPALVFFARIPVKAAIGTSLLIISANSLSGFAGDLTGDYIIDWKLL
                     LIFLGFAIAGILVGSRLTKFVSGKNLKKVFGVIVLVMGVYIIFRELSELNIF"
     gene            complement(18510..18980)
                     /locus_tag="PLX80_03490"
     CDS             complement(18510..18980)
                     /locus_tag="PLX80_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQY19891.1"
                     /translation="MKIVLIDGNNVIHKSAQFKKIFMNDKIAARQSFVEKVKSHLGRG
                     CRIEFYFDGHSEQNLSGIYFSGNKTADDLIRNRIERSEDHRNITVVSSDNGITGLARV
                     CGCTVKSSEEFLKELEGKENIFKGKNINHLLEKDNEKPNSVSRKDLDYFRDKFS"
     gene            complement(19426..20763)
                     /gene="asnS"
                     /locus_tag="PLX80_03495"
     CDS             complement(19426..20763)
                     /gene="asnS"
                     /locus_tag="PLX80_03495"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQY19892.1"
                     /translation="MKRVYINKISDHVDQEVTLKGWLYNIRSSGKLMFPEFRDGTGII
                     QGVVVKSAVSEKVWEDFSKLTQESSLSVKGTVSKHPKKDNVYELQVNDIEIINIAEPY
                     PITPKEHGVEFLMDRRHLWMRSPRQVAILRVRNEIIQSIRDYMYNNGFVLFDTPIFTP
                     NACEGTSTLFATDYFDMGKAYLSQSGQLYAEAGALALGKVYTLGPTFRAENSMTRRHL
                     TEFWMMEPEVAFCDIDEDMDLIEDFMEYVVQRVLKNCNTELTELGRDLSKLENVRKPF
                     PRITYDEAIEILIKKDIPLIRKTANGEEHIRPFPWGEDFGAPHEEAIVEDFDKPVMVH
                     RWPAIAKSFYMKRDPNNPEIALGVDVLAPEGFGEIIGGSQREDDLKELEKRIIEHDLP
                     MEEFQWFLDLRRYGSVPHSGFGLGIERMVNWICNLENIREAIPFARRPNRIRP"
     gene            21071..21673
                     /locus_tag="PLX80_03500"
     CDS             21071..21673
                     /locus_tag="PLX80_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19893.1"
                     /translation="MNKTFYIILSIFLFSSTGVYSQNPNEQITDVEKTQKKIMTWALV
                     QTIPSINWTNDRNSSNSQIVFGLKWNVTPINISFSTNKYVSPVQFFMVNPMRKFTGSL
                     EFFVQPEFAFSSLEYSGFDKFNVAVGSRVNIPVKNLGEHLYVSLGGKYNFRQNNLNNE
                     NGYYGVEGGVYVLFGLLGLTFNYNFNDGSEYAFGINFKYW"
     gene            21683..22681
                     /locus_tag="PLX80_03505"
     CDS             21683..22681
                     /locus_tag="PLX80_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY19894.1"
                     /translation="MLKIFFVLIIAVSLNGCGLFRIGLKNAGHTIFNNPDKPLKTEKY
                     PIRDSVKLSVMWAGHSSTLVQMYDKVILFDPVFNNVISGVMTRKYEAAINLTNVKNID
                     MILISHAHMDHLSISTLDDLEEKFSKAKLIVPMGAEEFIPGYDYDIVRLYTGNSSKKN
                     YVGQTYESNGIKVTAVYALHYGGRFGLDSYAWNMPGCTGFIVEYKDLVIFYAGDSLYE
                     EFAYKELGKKFDIDLALLPVGPCRDCEEDNSYTHMTSMGAFKVFDDLKADKMVPVHYG
                     ALEYSSDPDYPADVLRELIAKRDGENNSGQSGETYKDRIKIIDEGELFIFENVEKK"
     gene            complement(22781..23833)
                     /locus_tag="PLX80_03510"
     CDS             complement(22781..23833)
                     /locus_tag="PLX80_03510"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQY19895.1"
                     /translation="MNTEISIPEKVMYKALLEKDEEFEGQFFAAIKTTGIFCRPSCTA
                     RKPKPENVEYFKTPREAMVSGYRPCKVCKPLAHSREIPAEIKSLITEINNSPSLKISD
                     FDLRSRDLEPNRIRRWFLKNHGVTFQGFQRLMRINNAFHDIKNGDKVISAAFENGYGS
                     LSGFNYSFKKNADFNPNQSAAKPALTFTRFTTPLGPMMAVATEDGLCLLEFTDRRMLE
                     TQLKQLEKHFRSKIFPGKNKFFQETEKQMNEYFAGKRKEFDIPLIMSGTEFQKSVWKI
                     LQSIPYGQTRSYKKQAELLGNPKAVRAAANANGKNNIAIMIPCHRVIGEDGKLTGYGG
                     GMWRKKWLLDLEKEKL"
     gene            complement(24109..25869)
                     /locus_tag="PLX80_03515"
     CDS             complement(24109..25869)
                     /locus_tag="PLX80_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY19896.1"
                     /translation="MKIFTISFLALGILCFSGIVNAQTPSVTSFSPQRQIINADRSSQ
                     VIVNFNTAIDPASVNDVTFRVFEKLAGEAVGTFEFLNGNTQIKFTPSNPYLAGGWITV
                     SLSKGIKSQSNVPMEKGFVWQFWSKAGSGSLNLALINTIPVRRQGEGLIQCYGALGVD
                     LNNDRKTDLAIVNEVSRDFRVFLNNGVSFDTMYSIYPLPAGNYPSPSEGADFDHDGKI
                     DIVIGNAGNNIMSLFMATGDAVFQPETPYTAASNVRGVAVGDIDGDGDDDIVTANRGG
                     SNIAVFKNNGNGVFAPAQLINTVGNGETAVMLTDANNDGILDAFIGCYSSREIVLLLG
                     DGEGNFTFSARADLIGQPWAISVGDINGDGNADVSGALSSYNRIGVIFGDGIGGLSTV
                     TTYNTGSFPLAMDIGDIDGDNDLDLIASNYSGATFNVFNNTGAGVFSNSPIVLPASSS
                     GSCITVHDRDNDGDMDLAGIDEVDDLLFIFNNGTTAIEPNTNGTPEDFNLGQNYPNPF
                     NPNTKINYSLGVKGKVDLTVFDILGNEVTTLVNEVQESGNYSVEFSASTGNNYLPSGV
                     YYYRLSTEKFSDTRSMILLK"
BASE COUNT         8614 a         4537 c         4631 g         8819 t
ORIGIN      
        1 ttagtagtta atagttagca tttagtaata ttcacgtttc atgatactta attaatataa
       61 aaattatata cttaaaaact cactgctcga gatcatccgg acattcacaa aacccgaact
      121 tataaagttc gaagactttg tcaggtcgcc ctgttttaat aaaaaagata acgtaacaaa
      181 gctgtttcat gagataaaga aataagcttc aaaatttgta gttgagagtc ttgagaaaga
      241 gaagggtaac ccgtaactta attatccgtc tcgtctttga gttttttcag aatgttcatt
      301 gcatcgagag gtgtgatgtt gtcgatctca atatctgaaa tcattttcct tagagcttca
      361 tctttgatct ctgtcactgc agattcaaac atgcttatct gcagatcgtc cttggtcttt
      421 cttctatact ctttgtcctc gagggatctg agaatttcct ttgcgcgctt tgtaactttt
      481 tcggggacac cggccatctg tgcaacctga atgccgtagc tgtgatcggc agttccttct
      541 gtgatcttcc gcaggaatat tactttatca ttatattctt ttacctccac tctgaaattt
      601 tttatcctcg gatacatgtc tgccaaagca ttaagctcgt gatagtgagt agcaaaaagt
      661 gtctttgcat ttatgttcgg attttcatga atgtattctg tcattgccca ggcaattgag
      721 attccgtcat atgtacttgt acctcttcca atttcatcga gtaatataag actctttgaa
      781 gttgcattat tcagtatgtt tgcagcctca tgcatctcga ctaaaaaagt actctcgcct
      841 ttagcaatat tatccgacgc gccgactctg gtaaatattt tatctacaat acctatcttt
      901 gcactacgcg ccggtacaaa acatcctatc tgcgtcagaa gaattatcag tccgacctgt
      961 ctcagataac tggactttcc gctcatattc ggacctgtaa gaattagaat ctgactgttt
     1021 tttatatctg caaatgtatc attggatata aatttttcac cgggaggaag cagtctttct
     1081 attacaggat gtctgccatc gatgatctca agatcatcat tgtcactgac ttccggtaat
     1141 acataattat aatcttctga aacctctgca aagctgatca gtgtatcaag tgtagcagtt
     1201 aatgatgcat tcctttgcac ctgatctgtg aaatcgagta cgaaattttt taactcattg
     1261 aaaatatttt tttcaagctc ttctatcttc tcttctgcat tcaggatctt ttcttcgtag
     1321 actttcagct cgtcagtgat gtatctttca gcatttacca gtgtctgctt tcttacataa
     1381 tttgaaggta ctttttctaa atttgtcttt gtgacaatta tataatatcc aaagaccttg
     1441 ttgaaatcta ccttaagtga attgatccct gtagctgcac gctctttgct ttgataattt
     1501 tcgatccagg tctttccgtt tatgagaatg tcttttatat catcaagatc tttgtcatag
     1561 cctttattga tcatgccgta attatcgata cccgatacaa aattttcgtt gatgcagttt
     1621 tctaatttca gaataagttc atttatctcg atcagattag ttccgattgc ctgaacggct
     1681 gttgaaggaa atttcagaag ttcagctttt ataatattta tttttttaag tgagatctta
     1741 agctgaacta tatcccttgc tacagctctg ccggtagaaa ttttcgaaag aagtctttca
     1801 agatccgcaa ctgattttaa ttcctctgat atttttttcc gctcattttt attatcaaaa
     1861 aaatctttca ctgcaaataa tcttttctcg atctgctcct tttttttgag aggtcttgaa
     1921 acccattttt ttaaaagtct tccgcccatt gcagttttgg tcctgtcgag aattgaaatc
     1981 agagtacctt ctctgccgcc ttcagctata gatgaagtga tctcgagatt tctttttgta
     2041 gaaggatcta aaattatgta atcagaaaat tcataattgt aaattttctt tatatgagga
     2101 agattatttt tctgtgtctc gttcagataa ttaagtacac agcccgctgc gactgtagct
     2161 tcattcatat cttcgattcc aaaaccttta agtgactgtg taccgaaatg atttgtcaat
     2221 acttcttttg cataatcaga attaaagacc cagtcatcga tctttgtaaa gaaatgtttg
     2281 tttctctgtt cactgaatgc cgcatcggga tcatagtcaa gaagtttgta aattttttcc
     2341 ttatcttttt tggggattaa aatttctgtc ggatttatat tttcaatttg ttcacataag
     2401 ttttttgtca ggatttcgga agtcgcaaat tcacctgtag atacatcaca gaatgaaaat
     2461 ccgcagacat tatcttttat ataaactgct gcaagaaaat tgtttgattt atggtctaaa
     2521 agtttttcag aaaaatttgc gccgggtgtt actacttcga ctacatctct ttttacaatt
     2581 ccttttgcat acttgggatc ttctaattgt tcgcagactg caaccctgaa tccggctttg
     2641 actaatttag gcagatagtt gtcaagggaa tgatgcggaa atcctgcaag aggaacttcc
     2701 gacgcagcac cgttcgatct ttttgtaagt gtaattccga gaactttgga agcagtgaca
     2761 gcatcatttt caaaggtctc aaaaaaatct cccattctga ataaaagcaa agtgtcagga
     2821 tatttttcct tgatctttct gtactgtctc atcaacggag tttcattttt catatcatca
     2881 atatcatatt catgaaatat atttcatcaa aatagttttg taatagttta tttatttatt
     2941 tacaggttaa aaaaattgag aagtaaattt gtgtcagttt ctgctgcatg taattttata
     3001 aaaaaatcca attattaaaa tcacattatc aataatgttt tattttatct ttcaggtatt
     3061 tattgagcag ctctctccct ctgaagatat gtgcttttac ggtgcccagc ggcaggttca
     3121 gttctttcgc aatttcttca tattcttttt ccttcttatg tctcaataca attacatcct
     3181 tatattttga aggaagcgat tctattgctt catcaagtat gatcctgagt tgtttatcta
     3241 tcacattatt atcaggttta ttcccatgat caggaatttc aaaactatag tcactgtcat
     3301 cagcttgaat ctttttatca atagaaaatg tgtaaagctt ttttttccta aggtaatcga
     3361 ttgcgttgtt tgtcgcaatt ttaaaaagcc atgtggaaaa agcatactgt ttatcgaaat
     3421 tggcaagaga gttgaacgct ttgataaaag cttcctgagt cagatcttcc acatcctctt
     3481 tttttgagat cattctatag atcaaatgag tgatcatagg ctgatatttc ttcatgagtt
     3541 tcgcataaga attctgcctg ccggcaagtg cttcatctat cagcaccata tcttcggctt
     3601 ttgacttact cattccggat gaattactgt taatcttcat taagaaagtg agggtgtgag
     3661 gtaattaatt ttcattgtgt aaaaattaca tctatttgaa attaatttca actaattaaa
     3721 aagcatttaa attgaaatca gagttttata atttaaaata gaaaccattc caaatttaaa
     3781 ttagtctttg aaaaacattc tcaattttga tcttgctgca ctcgaaaaag aaattactga
     3841 atcaggtgaa cccaaataca gagctaaaca gattttcaca tcacttcaca aaaataatgt
     3901 aaaaaatata aacgagatca aaggtattcc taagtatctt aaaaataaat tagaagattt
     3961 atatgaaata cctgagctta aacttcttga agtcaggaac tcagaacagt ttaatacaaa
     4021 aaaatttctg tttgagatta atgatgaaaa taaaaatcac atattcgagt ctgtacttat
     4081 ttctgaaaaa ggaagacata cgatctgcat ttcgactcaa ataggatgta atgtcggatg
     4141 tgaattttgt gctacagcca agatgggatt tttgaaaaat cttgatactt cagagatcat
     4201 aatgcaggta tatgagataa tccgcaatac ggggatcatt ccgaccaatc ttgtatttat
     4261 gggaatgggt gagccgttcc ttaactataa taatctattg aattcgctga gaatacttac
     4321 cgacccggaa ggtctgtcca ttccgtcaaa aaagatcacg gtatctacag tcgggtttaa
     4381 agaaaagata aaaaaatttg cagatgatat ttcattggaa gagaataaaa acatacgaaa
     4441 agttaagctt gcattatctc tgcacacaac ggataatggt cttcgagaat ctataattcc
     4501 gacatctgcc aagaatcgtc tgtcggatct ttatgaagag ctgagttact tttaccgagc
     4561 tacaggcagt aaaatcactt atgaatatat ttattttcag ggacttaatg atacagataa
     4621 tgatataaag agattaacta aacttttaag aatggtacct tctaatttaa atatcatacc
     4681 ttttcacccg attgatttta aattaaaagc tcctttggat atttttgata aagataaaga
     4741 cctaaataat ttacttttaa ataaaaattt atttgatttt attgaaaaac ttaaattaaa
     4801 taaggtgact gtcaatctga gatcaagcag cggagttgat ataaacgctg catgcggtca
     4861 gttagcagta atcaaatcac agagttaata tttattgaat tctaatattt acgattaaat
     4921 gtttcagtta attatttttg gacctcccgg agtagggaaa ggtacacagg cagcatcagt
     4981 tgccgagaag ttaaatcttg cgcacatttc taccggtgaa atattgagga aagctgtatc
     5041 ggaagaaaca gaacttggta taaaagcaaa agagataatg gacaaaggta atcttgtacc
     5101 agatgagatc atgaatggaa ttgtaagaga gacacttgaa aaagtaaacg gtaatggttt
     5161 catacttgac ggatatccca ggacagttaa tcaggcagtt gcattagcag aaatttttga
     5221 aaagctgaaa ttttatgata taaaagtaat cactcttaca gcagatgata atgaaataac
     5281 ggaaagatta ttaaagcggg ggaggagtga tgatacaaga gatacaattt taaacagact
     5341 taaagtttat tataactcaa catcacctgt aaaggaatat tatgaaaagc agggaataaa
     5401 catggatgta aacggagttg gagatataaa agaaataaac gaatcaattt taaaatcagt
     5461 taattaaatt aaaaaaataa ttttggattt tgtaaaaaaa tttaatattc taatttttaa
     5521 taattgcaat tttaataata aacgtatatt ttgtaatcta aaatttttta tatatggcat
     5581 caataaaaga aactgaatta tcggataaag gaattgttat cctctctcct aagggaaatt
     5641 tcgtcggtgg tgatgagaca gatgagctta gggaagctat aaaaaagtat acggatcttg
     5701 gtaataaaaa attattgata gatcttggag aagttttata tcttaacagt actgccctcg
     5761 gagtactaat cgctgcacat gctaactatt caaaaagaga aggcaaaata aaattgtgcc
     5821 agctcaataa aaatctggaa aatctgtttg ttataacaaa acttgctctt atttttgact
     5881 cccatacgac tcaggaagag gcattgaaca gttttgaata aaaattaaaa ttatctataa
     5941 attaaaaatc taactctatc agggttaacg gaggttaaaa attaatgaaa cagtctgtat
     6001 ttatgtctgt agttctcatc acatccattg tggtttctgc tgcaatcttt tacggtgtgt
     6061 ttggtagtcc aagtaatttc aaaacacctg aaatgcatac accatcaaat atcatgggtg
     6121 ctatctacca gggtggtcct gttgttatag tattaatggc attaagtatt atggttataa
     6181 catttacaat tgaaagacaa ttgtcattat caaaagcaaa aggaaaaggt ccaattgagc
     6241 cgttccttaa aaaagttcag acttctttat tggaaggaaa cattgaggaa gctattgcgg
     6301 agtgtgataa gcagaaagga tctatagcaa atatactccg gcaaggtttg gataaatacc
     6361 aaatgctttc aatggataat aaagtagaat cagatacaaa gattcaggaa gttcaaagtg
     6421 caattgaaga agcgatgatg cttgaagtcc cgcttcttga aagaaattta gttgtacttt
     6481 ctactattgc ttctgtgtca gtgcttgtcg gacttttcgg aactgttatt ggtatgatca
     6541 gagctttcca ggcacttgca gctgcaggta ctccgaatgc cgctgaatta tcatcaggta
     6601 tctctgaagc tcttattaat actgcaggtg gtttgtttgg agctatcgta ggtacaattt
     6661 cttataacta tttcgttact aaggttggta atattacata catgatcgac gaagcaactt
     6721 ataatatgct tcagatcctt acaattaagg attcaaaaag atcagtataa gtaattcaga
     6781 aatccaactt aaaaatttac acaaatgaaa aaagccagat cgataaaaaa gccgggtgtt
     6841 aatatagata tgacaccatt agtagacgta attatgttac ttctgacatt ctttatgtta
     6901 gttgcaacat tcaaagcgga atccactgaa gcaattgaag ttactcttcc cgaatctgta
     6961 gttacagata ctaccaaatt acctgaaaaa gatataatga cacttactct tacaaaagca
     7021 ggcgatgtct ttgttgatgt tgacagctat aaagtaaaag aagaagtgtt tggaagctcg
     7081 atcggtattg gtttgtatca ccctgatagc acttcaaaaa gtgaatatga acttacaggc
     7141 ggaagcataa acggtaaacc atttaaaaga cctgttacaa tgctgaataa agaggagttc
     7201 gaaaaaactt taaatgacct tcgtatgact ttgaaaagtc agaacggcat gagctcagat
     7261 ttcagaatag ttttaaaagg agatatggaa actaactttg gtgtagttga agatcttatg
     7321 gaaagtttta aaactacaag aaatacaaga tttgcactgg ttacagatgt aaagtctgat
     7381 caaccggata attaatattt aaaaatataa aataatttta ttatggcagg cggtggattt
     7441 gatgccccgg actccggagg cggattacga aagcagaaaa aatataagaa aggaaagaag
     7501 atcggtgtca gaattgatat gacacctatg gttgacgtga tcatgcttct tttgaccttc
     7561 tttatcctta ctactacttt gaatacacct cagatcatgc agatcaatct tccaaaaggt
     7621 gatcaggaag ataagataaa agttgatatg ggtaatgtac tttatgtcag agtttctgaa
     7681 aatgcaaatg catatttctc atctggactt tcagacggaa atgaagctga accggtaaag
     7741 gtatctttta atgatatgaa aaataagatt gaagatcttt acagacaaaa tcctcagtta
     7801 ataattttgc ttaaatttga tagaaatatg aagtacagca caatgatcga tatacttgac
     7861 gagatcaata aggctgaaat tgataagaga tacagtttta tgaaaatgga agatccggat
     7921 aaggaaatag ttacaaaagc cggaggttaa tacttagtag aaagtttatt aaaagcggtc
     7981 tgaataaatt ctgatcgctt tttttattac aataaaataa tttatctgct gaaaaagcag
     8041 tatatgaaaa aaccattttt taaatattta ttcttacttc ttgtatttct tactttgtac
     8101 ccggagcttt attctcaggg acaaaaggaa agatctcttg ccgataaccc cattcagctc
     8161 aaagaatatc ttgtcgatga gacaggtatt ctgactcctg tgcaacataa agatattctt
     8221 gtaaaattag tcaatgaaga taagactaca tcaaatcaga tcgttgttta tattataaac
     8281 agtctaaacg gtgaatccct tgaagatgta tcgattaatc tggctgagaa aaataagatc
     8341 ggaaagaaag acaggaataa cggcgtctta atgctcgtag taagaagtga cagaaagatc
     8401 agaattgaag taggttatgg cttagaaggt gcgctgactg acgcaattag ttcaagtatt
     8461 attagaaatg tgatcacacc tgaatttaaa aaaggtgatt attataaagg aatcaatgac
     8521 ggagtagatg caataattgc tgcaataaaa ggcgaatata ctgcagaaga aaatgaactg
     8581 atttcacagg attcaagtaa atgctgtttt ggagtgccga tatttgttat gatgatattc
     8641 tttttcattt tcatttcaat atttacttct gtcctgagaa ggatgtttgg atttggtaat
     8701 tcagttcgta ccggaaaaaa agattcggga agcagctggt ggagcggcgg cggcggaagc
     8761 ggcgggggat ggagcagtgg cggaagcagt ggtggattct caggcggcgg tggttcattc
     8821 ggcggcggag gagcaagcgg aagctggtga tttttgctta atataaagta aataatattt
     8881 atcatgccaa aaaattatat aaataaatat ttatcagatt ctgatcttga tgaaatagta
     8941 aaaaatattt ctcaggcgga aagtaaaact tcaggcgaga tcagattgag tattaaaaca
     9001 aatagaggtt acagggaaaa aggtattact cccagagaac ttgcaattgc tgaatttcat
     9061 aatctggata tgcataaaac aaaagataaa accggtgtgc tgttttttat tttatttgac
     9121 gaacataaat ttgagatcat tgccgatgaa ggtattaatt ctaagatcga agattcagaa
     9181 tggaaaacat tttcagaaaa aactgcagat cttttcagag aggaaaagta taaagaagga
     9241 atcaaatttg taattgataa aataggagaa agattagctg ctgagtttcc ggttcaaaat
     9301 gatgataaga acgagctgtc aaatgaagta attatatcct gacaatattt tatttatatt
     9361 ttgtaagatg aaagttcgtt atatttatca aaattatttt ttcatttgaa cgactccgaa
     9421 atacttctca gaaaatatat taaaaacgaa aatcttatta agcactgcta tgccgtagaa
     9481 tctgctatga aatattatgc gggattgctt ggcgaagatt ctgaaaagtg gggagcagca
     9541 ggtcttcttc atgatctgga ctgggaaatg tatccggata ctcatccaaa tactgctgta
     9601 ccaattcttg aagaagcggg ctatgacaaa gaattcatag acgcaatcct tggacatgcc
     9661 tatcccgata ggactgatgt gccgagagaa acccagcttg ctaaatattt atttgcatgt
     9721 gatgagatca caggatttat tatggcttat tcattgatga aacaaaatgg tctgattgat
     9781 gtagagtcca aaagtattaa aaagaaaatg aaggataaag cttttgcaag gaatgtaaac
     9841 agggaagaca ttgtaaaagg tgctgaggat atcggcatag agcttgatgt ccatgtaaat
     9901 aatattatag aaagtatgaa gcaggataac agaataaata acagaaatta aatcatgaat
     9961 ataataaaca caaaagatct gtctaaagaa tattcatcaa aaagtttttc taaagataag
    10021 atccttgcat taaataattt ttcctttgaa gtggaagcag gggaaatatt cggaatcctc
    10081 ggtccaaacg gcgcaggtaa aacaactttg ataaaaatac ttctcggcat tactcacagg
    10141 acaaaaggtg agatcaatat attcggaaag gaaattaatg acgagtcata taaattgaag
    10201 gttggatacc tgcctgaaaa tcataaattc ccgaattatc ttaccggcga agaagtgctg
    10261 actttctacg ggaagatgag cggattaaat aaaacagaag taaaaaagag atctgatgaa
    10321 ttcctgaaac tcgtcgatat ggaaaaatgg agaaagacaa agatcaaaaa atattcaaaa
    10381 gggatgctgc agagacttgg ccttgcacag gcattgataa atgatccgga actgattttt
    10441 ctcgatgaac ctacagatgg cgtcgatccg atcggaagaa aagaaataag ggacgtcctc
    10501 gaaagtttga aagaaagagg taaaactatt tttcttaact cacatctgct tagtgaagtg
    10561 gaaatgatct gcgacagggt agctattatg aataannnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnngaaaa catgtttata aacttaatcg gaagtcaaaa ttagaatatg
    10741 atattttcaa cacttgtatt aagtacattc agggaagcaa tggcgaagaa aatattcctc
    10801 ggtttttttg cgatctcgac aattatcata ctgatatttt tatttctgat aaatgtagat
    10861 tcggtcgaag gaatggtaga tatggtcggt gcttcaggtg aagaggggat acggaaactt
    10921 gtgataggct ttgaagttgc gatgatagac atttcttatt tacttataat tactttctgt
    10981 ctgatctctg tttcatcttt cattccgtca atgctcgaaa aaggcaatat tgatctgttg
    11041 ctttccaagc cgatctcccg aactaatatt atacttgcta aatttatagg tggtgtctta
    11101 ctcgtcttca ttagtctgat atatcttata ggatttgtct ggatcatact ttcttcaaaa
    11161 tcaggcttct ggcatttccc gtttctttat tctatactat ggctgacact ttcatttgct
    11221 gtgatctatt ctttaataat tttcataggg ctgatttcgc aaagtacaat tctctctata
    11281 cttgtaagtt tctttcttgt gtttatcgtt tgtccgatac tttatgtaag agaagctaca
    11341 attttttctc ttgtggagaa tagtgctgca cagtttgtca taaacttttt ttactatata
    11401 ctgccaaagc cgtctgatat aaataaaatt acaacggata ttataacagg cgaagctgtg
    11461 aattcttaca tgcctgtttt ttcttctgtt ctttttatgc tggcagtttt atcagcttct
    11521 atattctact ttaagaaaaa agattattaa catctattct tcttatcaat gaaatttgta
    11581 aaaacttttc cggtactttt cataattgta actatcctga tacagggttg tgatttcaaa
    11641 cccgagccgg aaccgcttcc gccgatggta agagctactc ttaatctcct ccaggaagac
    11701 cctcagtttg taatgtatct gaattttaaa accatgagca ggacaaactt ctggaaaaat
    11761 aatatttccg attcccttct gaatgaggaa aaaacttttg gcagcgttct taatactttt
    11821 aagattgcaa caggagcatc tgtttctgac ggtcttgatg aaatgtatta ctcgaattca
    11881 tggttcggtg aaaactccat tgtactcaaa ggtctcttca ataaaaaaaa gcttgatgaa
    11941 tatcttctca ctgacagcat tttctcgatc acaaaaacat cttcaggtaa ggatatttat
    12001 ataaaaaatg ataatggatt atatttcttt tttaaaaatg aatttaccat ctgcgcaagt
    12061 aattatctca agcagatcga tataatgatg caggtaagtg atactgtaaa ttcaggactt
    12121 atgaaaaatg aaaagctgct cgggtcaatt gaaaatatta tttataaaca gaatctgtgg
    12181 atggtctcga ctgaaaaagc atttataaaa gggatctatc agaattttct cggatcgaca
    12241 gccggaagaa aacttgaaga agatacaaca gcagtcatga aagattcagt gaaagcaaat
    12301 gatgaaaaag ctgatctgga taatttatat acaaaatata attcgatctc attttcggca
    12361 gatatgaagg atgatattga aatacttata cagaacaatt tcattgatga cccttcagca
    12421 aaatttttta aaagcattct gaacggattt ctgactgttt ccaagttaag ttcgggtgga
    12481 gataaaaaac caccggcttc gaaactatta gataaaatta aactcagcag atatgataat
    12541 gaagtgttta taaaaataat tatggaaaac aaagatcttg aacagcttag gaattctgat
    12601 ctgatgagtg aacctggatt ttaatttatt aaaaattaat tcaatttaga atatcactgg
    12661 ataaattaaa atttaaagcc ggggcatttt gtcggttttt ttttaaatta ttaaatatat
    12721 taaattagat atgtaagata ttttggacta ttccaatcaa aattaaattg taatgttgat
    12781 cttaatttat aaatatagta ctgttttata aatctgactt ctttctaagt attattaatt
    12841 taaaaaggtt aattaatact taaattaaag agtcagattt tattttttag ttcaattttt
    12901 tgtagtgtac ttagttaaat aatacagtgg aaagtatttg ctgaaatact tatatatata
    12961 tcgctattat gttcaaaaca agtagagttt ccaataacaa attaagaatt ttgaaacaag
    13021 aaagtattaa atattttgat gaatgaaatg gcaactatgg ttaatgaaaa tattattcag
    13081 ataattactc ctgtgaacta tcaaatgtca aatatcgagt tgttaaattt ttataaccca
    13141 actgaggaaa atctatcagc taaaaagcgg gtgattctct ttaaaaaatc taataaactt
    13201 tgaaaataat ttttgtttta acaaatttat acatagtatt aaactgttct gaattacttt
    13261 gtcaggtaaa tgaagattgg acaagtattt ataacagtag tggaacatat gaatcaggta
    13321 ttagtgtgaa aactgataac tcaggaaatg tttatacagc tggtacgata caaacttcaa
    13381 ctggttttga ctgtatacta attaagtata acaattacgg caacgagatc tggacatcta
    13441 tttatgataa tcctctgtca aatcttaacg atggagttac ctcaatggta ttagatggga
    13501 ggggaaatat ttttataacg ggaactacat ttagttttga aaatgatgtt gattatctga
    13561 ctattaagta tgattcttcc ggagatatga agtgggttag aacttttgga gataccataa
    13621 tctggggtaa tgatacaact acttttttat cagattatac cgaagatatt gaagtagata
    13681 attttgggaa tgtctatgtc acgggagacg atggaacgat taaatacgat tcattgggaa
    13741 atctactttg gattgtaaaa gacgatatta ctattcagga tatgacaata gacgtaaata
    13801 gtaatatata cattacggga tttagatatg accaaaacac acacagagat tgcagaacaa
    13861 taaaatataa ctctttaggt gttagaattt ggatatctga ttatatggga ccaacttaca
    13921 gtgatgatca gggattggca gttactgcgg atgatttagg aaatgtatat gtaacagggt
    13981 ttacacattc aaacaattac agcgattatt caaaatacct tacactcaaa tatgacgaat
    14041 ctggaatcca acaatgggct gcaatatatg gtgaacaata tgaaagaaat atagcaaatg
    14101 acatagaagt tgataaatcc ggaaatgttt atgttactgg taatgcattc agtagcaatc
    14161 ttactagtaa tgattgttat ggtactatta aatacagtcc gtcaggttta caactttggc
    14221 ttacgctaca taccggcagt tctacggcca gagcaagaga tttagagctt gataatcaag
    14281 gaaacatcta tgtaacagga tactttggca gctctatagg agatgattat tctactgtta
    14341 aatataattc aaacggtctg aaaatttggc aggtacgttt caatggatca tttatttcaa
    14401 gtgaagaact aaggtcaatt gcaattgata atgtgggtaa tgtatatgta acaggtgatg
    14461 cttttccaaa tcttaatatg gatatagcaa cagcaaaatt ttcacaaaca attacgggaa
    14521 tttctgaagg tcaagggtta cttttatcaa gtttttctct ttcacaaaat tatccaaacc
    14581 cgttcaaccc gatgactgtt attcgatatt ctatcccgtc aaatttcaga ggacaggtat
    14641 catatgtaaa gcttgttatt tttaatacac ttggcatgga gatcgccact ttagttaatg
    14701 aaatacaaaa tacgggtgaa tatgaagttg aatttgatgg ttccagtctt acaagcggcg
    14761 tttattttta tataatagaa gttgattcaa gagtgattga tacaagacgt atggtgcttt
    14821 tgaaatagtt acaaatgtct atatagtaaa taagaactac cattaaccag atagaaggtt
    14881 gcttgaaaat cttaaacatt ttttgatttt agtaagcgaa gcgagcgtga ccaacaacct
    14941 aaactagtta gaaaatctga tcaaaataaa ttccttttct aattattcct cacttaaata
    15001 tttttccgcc agcaatacat cttcttcact gccgatataa agcggggttc tctgatgaag
    15061 actttcggga atgagatcaa gtatccgatt cttcccgtcc gaactcctgc cgcctgcctg
    15121 ctctactata aaactcaatg gattggcttc atacataagt cttaactttc cattcttatt
    15181 tttcagatca gccgggtaaa taaaaatgcc tccgtaaatt aaatttctgt ggaagtcagc
    15241 taccagagaa ccaacatatc ttgatgtata aggtctgccg gttgattcgt catcttcttt
    15301 cagatgattg atatactttt tcattccgtc agaccattta caataattac cttcgttcac
    15361 agaataaatt ttcgacttct ttggtatgga aatattttcc gttgacaata aaaactcacc
    15421 gacactcgga tcaagtgtaa acaagtgaac acctttaccg gctgtataag tcagcactgt
    15481 gcttgatccg taaatgacat aacctgctgc cacctgtaca tcgcctttct gcagacagtc
    15541 ttcaagtgct ccgggacctg attctgtaac tctcctgtaa atggaaaata ttgtcccgat
    15601 ggaaacgttt acatcgatat ttgaagagcc gtcaagcgga tcaaaatggc atatgtattt
    15661 atttgataca gaattatctg atccaaaatt cttatttatg ggtatgaaat tttcttcctc
    15721 ttccgaacaa acagcgcaga tatgtccgcc ctgtttcatc acattggtca gtacatcatt
    15781 tgcaaataca tcaagctttt ttacttcctc accctgtaca ttctcatcac ccgtaaaacc
    15841 aagtatgtcg atcagtcctg ctctgtttac ttcgagagaa attactttgc aggcaagagc
    15901 aatgtcggct aataaatctg aaagctgtcc tgatgcatcg gggaaatttc tttcctcttc
    15961 gataatgtgt ctgtaaaaag tattaaattt gctgaccata gattattcag ttatattttg
    16021 aaaagttttt tatctcgtca ctcaggtcaa atactttagt atctaattta tctctgagta
    16081 tgctggaagc cgcagcagac ctgtatccgg cagcacagtg aactacgaca ggcttatctg
    16141 aaggtatttc attgtatctt tctcttatct cgttcagcgg aatgtgaatt gagtcttcga
    16201 atattatttc cgaatttctt tcggataaat ttcttacatc tacaatagta aattcggaca
    16261 ggttttcttt gaaatcattc agatcaagtt tttctacttt cacagagtct tcaatctctt
    16321 catgtaccag tgcaccttca ataattaatt catatccgat ctttgcacat cttctgataa
    16381 gtgtatcgag tttttctttg ctgtcagaaa ttaaataaaa cttttcgtca ggtgagacta
    16441 ctgttccaag ataggtttca aattttgtgt cttctcttaa gtttactgca cccgggtaat
    16501 gtccgttttc aaaatcttct ttagatcttg tgtctataat tatatgacca tcttcaatat
    16561 cattatatga agaaatgatc tttacattgt ttacactctc ttcaaaatcc ggtgcgcctt
    16621 ttctgttcag ctctacatcg taaccaaaat attttggaat gtaagactgc tcatttaaaa
    16681 gttcatcaac aaatttatct tcactcatcg gctgaagggc ataattactt tttctttctt
    16741 tacctatagt tgaggaattt tccgtactgg ttgactttcc gcagagcgat cccgctccgt
    16801 gtgcaggata gacaagcaca tcgtcattta atgtaaggag tttttcattt attgtattat
    16861 acatcatccg tgcaagttca tctttggttt tatttataga gcctgcagat tctctcagat
    16921 caggtcttcc gacatcacct acaaataagg tatcgcctgt tgcaatcgca tgatcttttc
    16981 catcttcatc ttttatgata attgaaatgc tgtcaggtga atgacccgga gtgtttatag
    17041 gaaagagttt tattattcct aatgaaagtt catcaccttc atcaaaagta atatgatcat
    17101 acaaagcatt tacaagtttg cttatataaa tcggcgcacc tgtttttttg gaaatttcca
    17161 gatgtgaact tacaaagtca gcatgaggat gtgtctcaat tatagctttg atctctgcat
    17221 tattatcatc tgcaaaatca aaataaatcc cgggatctct tgctggatct ataagtgcga
    17281 tctcgttttc acttaaaatc gcgtatgacg catgtgcaag acttttatca taaaattgct
    17341 ttatttccat ttcaattatt attttattgt ttcaaaatta ttttctaccc atgagattat
    17401 cccctcggaa agattattaa cttcttcaaa gccaagatct ctcataagaa acattgcctg
    17461 tctgcttctg tttccggagc ggcaataaat aagatatttt tttctcttat ccagctcatc
    17521 cagtttatcc tcgaaatcag attcgtaaaa atcaatatta atcgaatcct ttagcctttc
    17581 agaattgaat tcatcaggtg tcctgacatc gagcaataca taagcatcat tctctgaaag
    17641 ctctttaaaa gactttgtat ctatatcttt atgctgcata ataaaaaatt taaattatta
    17701 aaatatattt aattcggaca attctctgaa aataatataa actcccatga ccagtacaat
    17761 tactccaaat acttttttca gattcttacc tgatacaaat ttagtaagtc tgctgccaac
    17821 caaaattccc gcaattgcaa atcctaaaaa tatcaaaagc agtttccagt ctatgatata
    17881 atcacctgta agatcgccag caaatcctga taaagaattt gctgaaatga tcagcagtga
    17941 agtacctatc gctgccttta ccggaatcct tgcaaaaaag actaaagcag gtacaatgag
    18001 aaatcccccg cctgcaccga ccattcctgt tacaaatcca actgcaaaac cataaaaaac
    18061 aagtatgaga ttttcattca ttgtcatact gtgatgatca tctttttcct ttacatttga
    18121 aaaaatcata gacaatgaag aaaaaagcat gatcactgca aagagcaaaa gtataaaaat
    18181 atctttggag ataataagat ctccgctttg ataaattact tcaggtaaag aaggcagtat
    18241 atatttcctt gttaaaaaaa tcgaaacaag tgaaggcact gcaaatgcta ttgatgtttt
    18301 tatatttaca agaccttgtc ttatataaga aacagaacct atcagagtcg taaagccgac
    18361 tacaaaaagt gaatacactg ttgcaagaac cggatttatt cccataagat ataccaatat
    18421 aggaactgtt aagatcgaac cacctgcacc gatcattccc aaaactaaac ctacaaatac
    18481 agcagcgagg taaccatata tttcagacat cagctgaatt tatccctgaa ataatcaaga
    18541 tcttttctgc tgacagaatt tggtttctca ttatcttttt caagaaggtg attaatattt
    18601 ttacctttga aaatattttc ttttccttca agttctttta aaaattcttc tgagctttta
    18661 actgtacatc cgcaaactct tgccagtccg gtaattccat tatcagagct gacaactgta
    18721 atatttctgt gatcttcaga tctctcaatt ctgttcctta tgagatcatc agctgtttta
    18781 tttcctgaaa aatatatccc gctcaaattc tgttcgctat gaccgtcaaa ataaaattct
    18841 attctgcatc ctctgcccaa atgagatttt actttttcta caaaagactg tcgtgcagca
    18901 attttatcat tcataaaaat ttttttaaac tgtgcagatt tatgtatcac attatttccg
    18961 tctatcaaaa ctattttcat tttctcattt tcacatttag aaatgtatta aataactttt
    19021 acagattcag ttcaaatcta aaattcatta aggtcatatt caagtattgt ccaaaatact
    19081 ttttataaaa atattctatc aaaatataca ttaaacattg aaaataaatt gtcctttctg
    19141 attaccccac ctttgcccta atgccattaa gttaagggcg aaatatcgcg gctgtcagca
    19201 aacctttccc aacctccatt ttgaaaaaaa ggagaggagc tgtaattttt aaactaaata
    19261 tttatagttt taaaattact attaaaaatt cctttaactt attgcccctg ccctccccgg
    19321 gtaaggggag ggcttggaga ggtttagaca gaaaaatgaa cttctaaatt caaaataaaa
    19381 ttaatctaac ggattaaaaa attttaatca tatcaaattt tacacttaag gtctgatcct
    19441 gttaggtctt ctggcaaacg gaattgcctc tcttatattt tccagattac agatccagtt
    19501 taccattctc tcaataccaa gtccgaatcc tgaatgcgga acactgccgt atcttctcag
    19561 atccagaaac cactgaaatt cttccattgg cagatcatgt tcgataattc ttttctcaag
    19621 ttctttcaaa tcatcttctc tctgtgagcc gccgataatt tcaccaaaac cttcaggagc
    19681 gagtacatcc acaccgagtg cgatctcagg attatttgga tctctcttca tataaaatga
    19741 ctttgctatt gcaggccatc tgtgcaccat aacaggttta tcaaaatctt ctactatagc
    19801 ttcttcatgg ggagcgccaa aatcttctcc ccatgggaaa ggacgaatat gctcttcacc
    19861 atttgcagtt tttctaatga gcggaatatc ttttttgata agtatttcaa ttgcttcatc
    19921 ataagtaatt cttggaaaag gctttcttac attttcgagt tttgacagat cgcgaccgag
    19981 ttcggtaagt tcagtattac aattcttcaa aactctttgc actacatact ccataaaatc
    20041 ctctattaaa tccatgtctt catcaatgtc acagaatgca acttcaggtt ccatcatcca
    20101 gaattctgtc aggtgtcttc tcgtcataga attttcagct ctgaatgtag gtccgagcgt
    20161 ataaactttt ccaagagcaa gtgcacctgc ctcagcataa agctgtcctg actgggaaag
    20221 gtaagctttt cccatatcaa aataatccgt tgcgaaaagt gtcgatgttc cttcgcaggc
    20281 attcggagta aaaatagggg tatcaaaaag tacgaaacca ttattgtaca tgtaatcacg
    20341 gatagactga atgatctcat ttctgacgcg gagaattgca acctgtctcg gagacctcat
    20401 ccatagatgt cttctgtcca tcagaaattc tactccgtgt tcctttggag tgatcggata
    20461 tggctcagca atattgatta tctcaatgtc attgacctgt aattcataga cattatcttt
    20521 ctttgggtgt ttcgaaacag ttccttttac tgaaagcgaa gattcctgtg taagctttga
    20581 aaaatcttcc catacttttt cagacacagc actctttact actactccct gtatgattcc
    20641 tgtaccatct ctgaattcag gaaacataag ctttcctgat gaacggatat tgtaaagcca
    20701 tccttttagt gttacttcct gatctacatg gtcagatatt ttatttatat aaactctttt
    20761 catttatatg gttgaattat taaaattaga tttcttcaat ttaacctttt agatgtatta
    20821 aataaattca gtactaaagg gattttaaaa aaaatttaaa tgtattgaaa aatcttaatt
    20881 gaattctttt atacaaaatt cagttcactt caacgtacat cttttataaa ttccatttaa
    20941 atatgatttt taacctgatt taatttaatt tcatatcatt atttaaagtg attttaaaaa
    21001 ttccaattaa tggtaaatga tttttaaatt aaaatagcta tgtttaaaat atactattcc
    21061 aaatttattt ttgaataaaa ctttttatat aatattatcc atatttctgt tttcttcaac
    21121 aggagtatat tctcagaatc cgaatgaaca aattactgat gttgagaaga cacaaaagaa
    21181 gataatgacc tgggcattgg ttcagacgat tccttctata aactggacaa atgacagaaa
    21241 cagcagcaat tcgcaaattg tgtttgggtt aaaatggaac gtgacgccga tcaatatttc
    21301 tttcagtaca aataaatatg tatcaccggt acaattcttt atggtgaatc caatgcgtaa
    21361 atttacaggc tcgctggaat tttttgtgca gcctgaattt gcattttctt ctttagaata
    21421 ttcaggtttt gataaattca atgtagctgt tgggtcacgt gtaaacattc ccgtaaaaaa
    21481 tctcggcgaa catctttatg tttcactcgg cggaaaatat aatttcagac agaataatct
    21541 gaataatgag aacggatact atggtgtgga aggcggagta tatgttttgt tcggacttct
    21601 cggacttact tttaattaca attttaatga cggatctgaa tatgcttttg gaattaattt
    21661 taaatactgg taaatgatat cattgctgaa aatatttttt gtacttataa ttgctgtttc
    21721 tttaaacgga tgtggtttgt tcaggatcgg tctgaagaat gccggacata ctattttcaa
    21781 taatccggat aagcctctta agacagaaaa atatccgata agagacagtg ttaaactttc
    21841 ggtcatgtgg gctggacatt cctcgacact tgtacagatg tatgataaag taattctatt
    21901 cgatccggtt tttaataatg tgatcagcgg tgtgatgacg aggaaatatg aggcagcaat
    21961 aaatcttacg aatgttaaaa atattgacat gatactgatc tctcacgctc acatggatca
    22021 tcttagtatt tccacacttg atgacttaga agaaaaattt tcaaaggcta aattaattgt
    22081 tccgatggga gcagaggaat tcattcccgg atatgattat gatatagtca gattatacac
    22141 agggaattct tccaaaaaaa attacgtcgg gcagacatat gaatctaacg gaattaaggt
    22201 tactgcagtg tatgcacttc attatggcgg aagattcgga ctggacagtt atgcatggaa
    22261 tatgcccggt tgtactggat ttatagtaga atataaagat ctggtcatct tctacgccgg
    22321 tgattcattg tatgaggaat ttgcttataa agagcttggg aaaaaatttg atatagatct
    22381 tgcattgttg cctgtgggtc cgtgcaggga ttgcgaagaa gataattcat atactcacat
    22441 gacatcgatg ggagctttta aagtatttga tgatctgaaa gctgataaaa tggtgccggt
    22501 gcattacggt gcgcttgaat attcttcgga tccggattat cccgctgatg tactcagaga
    22561 actgatcgca aaaagagacg gtgaaaataa ttcaggacag tcaggcgaaa catataaaga
    22621 caggataaag atcattgatg aaggggagct ttttattttt gaaaatgttg aaaaaaaata
    22681 agttctgtac gattttcaaa atccggcaat cttaaatcta ctttcttcga attatatttt
    22741 aagatttttg aaaataaaat ttttaaaatt atatccgtaa ttataatttt tctttctcaa
    22801 gatctaacag ccacttcttc ctccacatac ctcctccata tccagtgagt ttcccgtctt
    22861 ctccaataac cctgtgacat ggtatcatga tcgcgatatt atttttaccg tttgcatttg
    22921 ctgctgctct gactgctttg ggattaccga gaagttcagc ctgctttttg taggatcttg
    22981 tctgtccgta tggaatcgac tgtagaattt tccagacgct tttttgaaat tcagtaccgg
    23041 acattattaa aggaatatcg aactcttttc ttttacctgc aaagtattca ttcatctgtt
    23101 tttcagtttc ctgaaaaaat ttattcttac ccggaaagat ctttgatctg aaatgctttt
    23161 caagctgctt gagctgtgtc tcgagcattc ttctgtcagt aaactccaga aggcaaagcc
    23221 cgtcttcagt tgcaactgcc atcataggtc cgaggggagt tgtgaatcga gtgaaggtca
    23281 gagcgggttt tgcagcactt tgatttggat taaagtcagc attcttttta aaagaataat
    23341 tgaatccgct aagtgaacca tatccgtttt caaatgcagc actgatcact ttgtcaccat
    23401 ttttgatgtc atgaaaggcg ttgttaattc tcatcagtct ctgaaatccc tgaaatgtga
    23461 ctccgtgatt tttcagaaac catctcctga tcctgtttgg ctcaaggtca cgtgatctta
    23521 aatcgaagtc agatattttt aaggaagggg agttgttgat ctctgtgata agagatttta
    23581 tttcagcagg aatttccctg ctgtgcgcga gaggtttaca cactttacac gggcggtatc
    23641 cgcttaccat tgcttcacgt ggtgtcttaa aatattcaac attttccggc ttcggtttgc
    23701 gtgctgtaca tgatggtctg cagaaaattc ctgtggtctt aattgcagca aagaactgtc
    23761 cttcgaattc ctcgtctttt tccaaaagag ccttatacat aactttttcc ggaatgctta
    23821 tttctgtgtt cataattttt tttattacaa atatataaat gtattataag tcattccacc
    23881 gaaaatttaa caagcaattt ttaactgtga ttttaaagat tataagattt tcatgattga
    23941 gttatatttg aaaattgttg cttacggttt ctttcggcaa gaacccactt cgatccccct
    24001 tttttaaggg ggaattgatt ttaatttaat ctgaatcgtg cttattaaaa ttagaaaaag
    24061 tatctaataa agctttacaa ttcttaattt ctaattatta attcttaatt attttaacag
    24121 tatcatcgat cttgtatcag aaaatttttc agtactcaga cggtaataat aaactccgct
    24181 tggcagataa ttatttcctg tagatgctga aaattctact gagtaatttc ctgattcctg
    24241 tacttcattt acaagtgtcg taacttcatt tccaagaata tcaaaaactg taagatctac
    24301 ttttcctttt acacctaagc tgtaatttat ttttgtattt ggattaaaag gatttggata
    24361 attctgacct aagttaaaat cctcaggagt cccgtttgta ttaggttcta ttgcagtcgt
    24421 tccgttatta aaaataaata gtagatcatc cacttcatct atccctgcaa gatccatgtc
    24481 accgtcattg tctctgtcat gaacggttat gcatgaaccc gaactcgacg ccggaagtac
    24541 aatcggtgaa ttagaaaaaa ctcctgcgcc tgtattatta aaaacattaa aagtcgcacc
    24601 gctgtaatta ctggcgatca gatcaagatc attatcaccg tcgatgtctc cgatatccat
    24661 tgccagcggg aaagagcctg tgttgtaagt cgtcactgta ctaagtccgc cgatgccgtc
    24721 accgaaaata acacctatcc tgttatagct tgagagagcg ccggagacat ctgcatttcc
    24781 gtcgccgttt atatctccca ccgaaatagc ccaaggctga ccgatgagat ctgctcttgc
    24841 agaaaaagta aaattccctt caccgtcacc gagcagtaaa acaatctcgc ggcttgagta
    24901 acatccgata aatgcatcga ggattccgtc attatttgca tctgtcagca tgacggcagt
    24961 ttcgccgttg ccgactgtat tgattaattg cgccggtgca aaaacgccgt tcccgttatt
    25021 tttgaaaaca gcaatattac tgcctcctct gtttgctgtc actatgtcat catcaccgtc
    25081 tccgtcaatg tcaccgactg caacgcctct tacattgctg gcagcagtat aaggagtttc
    25141 cggctggaag accgcatcac ctgttgccat aaataaactc attatattgt tgccggcatt
    25201 gccgatcaca atatcaatct tcccgtcatg atcaaaatcc gcgccttcac tcggacttgg
    25261 gtaattgcct gcaggcagcg gatatattga atacattgta tcaaagctca caccattatt
    25321 cagaaaaacc ctgaaatctc gggacacttc attcactatt gcaaggtcgg tttttctgtc
    25381 attattaaga tcgacaccga gcgcaccgta acactgtata agaccttcgc cttgtcttct
    25441 cactggaata gtatttatca gcgcaagatt taatgaaccc gatccggctt ttgaccagaa
    25501 ctgccataca aaaccttttt ccatcgggac attactctga ctttttattc cttttgaaag
    25561 tgagactgtg atccatcctc cggcaagata tggattggaa ggagtaaatt ttatctgagt
    25621 atttccgttg agaaattcaa acgttcctac tgcttctccg gctaattttt cgaaaacccg
    25681 gaaagtcaca tcattaaccg aagccggatc aattgcagta ttaaaattta caatcacctg
    25741 cgaactcctg tcggcattga tgatctgacg ctgcggtgag aatgatgtta ccgatggtgt
    25801 ttgtgcattg actatgcccg agaagcaaag aatacccaaa gctaaaaagc ttattgtgaa
    25861 aattttcata atttaaaatt aaatttgaaa aatatgttta atataaaatg taatttcaat
    25921 ctattaaatt aattaatagg aatgattcat ttgcaaaccc gaattcattc ggatgaagca
    25981 atctgactct gcgatggtat tttaatttat aagattttca atgctcaatt aatagatcaa
    26041 ttcgatatag tttgagtgag ttatctgtga aaaatgtttt acatagtaaa agatgaattt
    26101 ggattatttg aaaattatat ggagatatgt ctttctattg agtccagtct cggtcttcgc
    26161 aggcactgtt gttggtgatc aaaagtttgc attagcagag caaaaaattt aaagatttct
    26221 tgtggcaaaa tttttaccaa accgctgcgg caatcgcagc ggtttttgtt tgtataatac
    26281 atttaatacg ttctgtaaca tcgtaataat taaaataaat ttttaacttg taatttaact
    26341 gaaatttatt ttcctttcat taaaaaataa ttaactgcca aatcgctttg aaatttttta
    26401 attaaattca aaatatgaaa aatggataat actttttatt aatgctgcct tcctttaaca
    26461 ccaatttatt taccccaaaa aaaatttaac ttttaaataa ttcattatgc tgaaataata
    26521 tataaatgaa aaaaataatt ttaatattta ctgtaattct actctcaagc caaggtgcag
    26581 aagcgcagtg gatacagcag ttaacactcg gacatccaat tagagatata gagtttttaa
    26641 acagaaatac cggctgggcg tgcggagata attttatata taaaactact aacggaggaa
    26701 t
//
LOCUS       DAORVQ010000023        26406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_656_length_26406_cov_5.664339, whole genome shotgun sequence.
ACCESSION   DAORVQ010000023 DAORVQ010000000
VERSION     DAORVQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 26406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26406
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_26406_cov_5.664339"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..642)
                     /locus_tag="PLX80_03520"
     CDS             complement(94..642)
                     /locus_tag="PLX80_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19897.1"
                     /translation="MFKNIIKGKKCSLIRFIGSDEKLVELMHNIFNDEELVEFLNPEY
                     LNYRTKPQIRKWLKSKYSNPVEIWYVIKHKNDYVGYICYKWRKHYDDACEISTAIDKR
                     FRGLDLGYESSKLLLDHLSSSKKFKYIVGYVHKKNKIAENNLKKLGMKKNNKLQKVVT
                     REFYDSDGSKSVYNLYSINTLK"
     gene            complement(674..2167)
                     /locus_tag="PLX80_03525"
     CDS             complement(674..2167)
                     /locus_tag="PLX80_03525"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY19898.1"
                     /translation="MLNYRLFINGEFCDSDDKQTFKATDPSNGEVIAEISKAGISDTK
                     KAVKAARDAFDSGIWSGLPGEERSRLLKQLVDKINENAEKLTELEVKDSGSTIRKAGE
                     DVFLSAKSLNYFSKAAAIDLNEEISGLSKEGVSKNIIRYEPIGVCALITPWNFPLKMA
                     IWKLGPSLAAGNTVILKPAMETSVTACELARLVSETDIPKGVINIITGSGSEIGTELV
                     KNPDVDKVGFTGSTEVGKIIMQNASINLKKITLECGGKSANIVLDDADLEMSIDGAIY
                     AAFYHQGQCCEGGTRLLLHEKIYDKFISGMKEKIERMKIGDPMDKSVTVGPVVNQSQF
                     DTVMKYIKTGIEEGAELLTGGKQSGDSGLFIEPTVFVNVNNKMKIAQEEIFGPVLVVM
                     KFKDDDEAVATANDSIFGLAGAVWSSNDERALKIADRLRAGTVWINEYHLLSDKAPFG
                     GYKQSGIGREFGMDGLKEYMEAKHIHIDEIKDRTKKPWYRTVVPKDI"
     gene            complement(2377..2859)
                     /locus_tag="PLX80_03530"
     CDS             complement(2377..2859)
                     /locus_tag="PLX80_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQY19899.1"
                     /translation="MVLNQNYEPLTICNVRRAIILIYLGKAESIYCLEAKKIYTVNRS
                     FEYPSIVRLVFFVRVPFKKIILSRKNILRRDNHKCQYCGVTSNLTVDHVLPKSRGGED
                     SWENLTTACIKCNNKKGNRTPDEAKFTLLNIPKRPSHITFIKNFAGRIDEGWKPYLYM
                     "
     gene            complement(3251..4435)
                     /locus_tag="PLX80_03535"
     CDS             complement(3251..4435)
                     /locus_tag="PLX80_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQY19900.1"
                     /translation="MNSLTSESISLLDEINSIEEARNLFPVTESCVYLDSAHYSQYSL
                     ETRRRLMEFIDEFTFTNKNLSLINMKLSETLKSRAADLINADKNEIIIIGSTTHGLNI
                     FANGIKLERGEKAAYADSEFPAVAYPWMNQQKLRGIENILIPSVNGKIKIGDIEKTIR
                     ENNVRVLTISSVEFLGFKNDLNAVGKICRENNCYFVVDAIQGMGVCPVDVKESGIDFM
                     AAGSQKWMMAPAGIGFAFISDRIRDNVDPTYAATSSIEFDFKNFLDYKLNFKKDGNAF
                     ENSTPNTLGMIGLISSIDLFLKLGVKNIFGHIQKLQDIFINEMDDSDFIIDSDPDPVH
                     RSNILIFSYKDNKRNDEVQKYLESKKIFIALREGFLRLSAHLFNNKKDITDLTDALKE
                     FS"
     gene            complement(4442..5413)
                     /locus_tag="PLX80_03540"
     CDS             complement(4442..5413)
                     /locus_tag="PLX80_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY19901.1"
                     /translation="MLIIVLLFYSYNESQIYSDSYSIRSGVNNINNNDAISPSPDKHE
                     KIEGLNNFISRKSFSSNMYIEDMFKDWNTLFDLFASNANFKQQAKNLNVKDLERISEK
                     VNLVSKEFKLGNVENLVKELGEVITRESNFYNLDWRIVLAIIRQESYFNANARSHAGA
                     FGLMQLMPRTGEGLQNTLKLEETQTPVNNLIAGIYYYASLVSQFRDYGDEKYKFALAA
                     YNAGLGRVIDAMTITAYFNKDHKQWENVKEAYPFLAAKEDSIHALVWPESQRPPYGTL
                     DNWKEPYNYVNSIMFYYESYKKMYESNLEEPKETKKTGTKKSKKNKK"
     gene            5950..6240
                     /locus_tag="PLX80_03545"
     CDS             5950..6240
                     /locus_tag="PLX80_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19902.1"
                     /translation="MSIEPTVCKCEENNMSDEVPADVSKMSCCDNNILEINNSNTLVK
                     NNTDYTNLNSIQLLISLLPHIEITKTFADHLVFQKHHKPPSDIPILISSFLI"
     gene            6331..7596
                     /locus_tag="PLX80_03550"
     CDS             6331..7596
                     /locus_tag="PLX80_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY19903.1"
                     /translation="MKYLIIHLILLPGLLFGQDVPQSNYPKTGLEDIIKYSLETNSGL
                     APLEYESKIMSARIDRTNFQPSPSFQFMLDFLPVDLSNAGEYIGIYSQPLRLFGKLDA
                     EENLAKVNSQRPEIEKTALENELIRSVKENYFMLSVNERLLSFNNEFKEIMKAVTSTL
                     EIKYSSGKGSQFDILKSNNEFQKLLLEEIELAGNKKIFINNLRTLSNLDLPYDFSTKN
                     IEILLNIPTIDMDTSKLISEMKLNNTDFKFIDQMLLVNQTEQNIIDLERKPDINLMTG
                     YKYKSDKKASFLMFSVMVELPFMPWNEKRIDAEIQEKIYRDKKINSESKSIELNLKNE
                     LKNNIVKINSSLEKIKYLNEVLIPQTEQTFRSSLLSYETDSNQFIDLLDTYRSLRENS
                     KMLAEEETNYLILISSLEKLIGKQILTIN"
     gene            7598..8947
                     /locus_tag="PLX80_03555"
     CDS             7598..8947
                     /locus_tag="PLX80_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY19904.1"
                     /translation="MNKTLIILITVLITTGLLFGAYKIFYDQGKTEELIAADELYICP
                     MHPQIQSDHPGVCPICNMDLVLKEKTNTELEFDSLKNLNKEISDLVLSPAQQILANVQ
                     TEKVIVKEFKSRKSFNGYVKSADNNMRFISTPVSGKIVKMYINYEGQTVSKGQRVFDI
                     YSPEIYSTQKEYLLALKNYESAKSSGNTLVIDQAESLINASKIRMSLWEITNEQISEL
                     EQSGEVKDFISVYSKYSGVVTKKLQNEGRWAMAGENILDITDLSTVWIIANVPESDLK
                     NISMGQNGMITSVAYPGEVFNAKVNFISPVFNADSRSLEVRFDVTNRNYKLKPDMYVD
                     VEIGSSDYQWNIVVPKNAVLRTGKMDMVYIKKSENLFSPKMVTVGGVSNGFYLITSGL
                     SEGEEVVTSAGFLLDSESQIRSGSNMNNMEGMEMEVKGDPEFNKDQDVMKDIQEHKH"
     gene            9000..9461
                     /locus_tag="PLX80_03560"
     CDS             9000..9461
                     /locus_tag="PLX80_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQY19905.1"
                     /translation="MKYINFKKTTVMIISIFLVTAFIYGCGEDKTGEVTEKAKEEKTT
                     ESKMESESHDMNNANMEHADIKLSSMQCGMCKKTIETAVKNTDGIQSIIVNKDEKVAH
                     VNFDKSKTDLKNIEATITAAGYDANDKKADSRAYENLDDCCKLPNDQKSKK"
     gene            9507..9872
                     /locus_tag="PLX80_03565"
     CDS             9507..9872
                     /locus_tag="PLX80_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQY19906.1"
                     /translation="MKSAISKNLKVIFAIFFMVTLIPMLSKADNKGNEKTVEITLPSI
                     QCGMCVKTVEKALGNLDGVIDSKADLDNKSVTVIYDDSKVSVENLEAVITASGYDANK
                     MPANEDAYNELNACCKKPK"
     gene            10109..10648
                     /locus_tag="PLX80_03570"
     CDS             10109..10648
                     /locus_tag="PLX80_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY19907.1"
                     /translation="MVCDRCIMVVKNELEKLGYMANHIVLGEATINEVLNDQEKAIIE
                     NNLKEFGFELIDDKNSSLIEKVKNLIIDLVHNHDNELKINLSEYLESNIHKDYTYLSN
                     LFSESQGITIEKYYIRQKIERVKELIVYDELTLSEISFNLGYSSVAYLSSQFKKITGL
                     TPGHFKKIKSIKRKSLDEL"
     gene            10767..11294
                     /locus_tag="PLX80_03575"
     CDS             10767..11294
                     /locus_tag="PLX80_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane or secreted protein"
                     /protein_id="HQY19908.1"
                     /translation="MKTIKKVTLTFAAITLIAVFSSCQSSPDVKEVLSKPETRNMIMD
                     SIANNSTMSMEMMESMMKSKSGNMMMQGDGKMMGKMMGMMMENHDGMMKMMKENPAMM
                     ESMMSKMMEMCKSDSTMMSGMCKTMMGDKQMMGMMQDMMKDDKSMNMTGSEKKMDKMN
                     DNNMNGMDHNKTEKK"
     gene            11341..13341
                     /locus_tag="PLX80_03580"
     CDS             11341..13341
                     /locus_tag="PLX80_03580"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HQY19909.1"
                     /translation="MDHSKMDHSSIPMGMEGHDHHQMMIKDFKLRFYVSVILTIPVLI
                     LSEMIQGFFGFHIQFPGSQYLVLILSTVIYFWGGKPFLTGFISEMKNRSPGMMTLIAM
                     AISVAYFYSAATVFGLSGMDFFWELATLIDIMLLGHWLEMKSVLGASKALQLLVSMMP
                     SEAHRVNPDGQTEDVKLDMIKKGDTILIKPGEKVPADGIITEGSSYLNESMLTGESKP
                     VKKESGNKVIGGSINGNGSLKIKVEHTGKESYLNKVITLVEEAQKSKSKMQNLSDRAA
                     KWLTYIALAVGFGTLAVWLFLGFPFVYALERMVTVMVITCPHALGLAIPLVVAISTAI
                     SAQNGLLIRNRTAFEESRKITVILFDKTGTLTTGVFGVNRFESISDISSEEMLRLSSA
                     LEQTSEHPIAVSIIEKVKELDIKIPAAENFNAITGKGVEATVEGKQIKVVSPGYLKEM
                     NITIPDKAFTNNAETVVFVLINDRLTGYISLSDVIRPESRDAINILKKNGIKVMMATG
                     DNETVAKSVSDELGLDGYFAEVLPHEKVQIVKDLQSKGEFVAMSGDGVNDAPALAQAD
                     VGIAVGSGTDVAAETADIVLVNSNPKDIAKLILFGKATYKKMIQNIVWATGYNTFAIP
                     LAAGVFYSSGFVLGPAVGAVFMSLSTIIVAINAQLLKKQMKQ"
     gene            13407..13847
                     /locus_tag="PLX80_03585"
     CDS             13407..13847
                     /locus_tag="PLX80_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQY19910.1"
                     /translation="MYKKFILMLSLSFVIMYAVMFLNIDDLSHIYLSLTRLYMSLLMV
                     SPMAILMLLLMPKMYTDKKLNAIILIVSVVVFIISLTFLRMQTIIGDKQYMLAMIPHH
                     SSAILTSQNADLQDPEVKQLAEDIIKAQEKEITQMKNILERMEK"
     gene            13890..14627
                     /locus_tag="PLX80_03590"
     CDS             13890..14627
                     /locus_tag="PLX80_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQY19911.1"
                     /translation="MTHKFKVTGMTCSSCAGKVQKTLSKVNGVESVTVTLDPPEAEIK
                     MHHHIEESTFNSALKNIGDYRLGDEVSSDHLKAMTETGEEVSKLKTYKPLIIVFLYII
                     LGVTILEIRSGSFDWMNAMNNFMAGFFLVFSFFKMLDLSNFAMSYSSYDIIAKKWYGY
                     GFVYPFIELTLGVLYLLQLNPVFTNMATIIVMGISSVGVIQSVMQKRTIQCACLGAVF
                     NLPMSTITIIEDLLMVAMALVMLMYLI"
     gene            14795..16432
                     /locus_tag="PLX80_03595"
     CDS             14795..16432
                     /locus_tag="PLX80_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19912.1"
                     /translation="MKLKYILLFLFALILKSGFTQPVEEWTQRYNGSANSFDIVSKIL
                     VDNDNNVYIFGSGNQTGSATDFLIIKYSPDGNILWTELFNGSGNSFDHINSACFDNSG
                     NIYVTGYTTDFESRNLITTAKFDTSGNFIWSNVFSKQGYIIGDGKDIIVDNSDNILVC
                     GSIKREIGFFDIEVIKYSPEGDEIWSNTFNGEGNGHDNPVTLKNDNLDNIIIAGTSKS
                     LSTDSDMIIIKYDSSSNLIWKKTFNGTADLGDVLTSMVLDTESNIYFCGNKFNTQSSY
                     DYFYAKLDQNGNIIWSGSYNGIGNSIDIPSSIILDSFNNVYITGFSRAGMDLGSEDFL
                     TIKLDQDGNTGWVRNFDGIANGMDQANSIAVDNYGNVYVGGGSDTGNIHLVYSLIKYD
                     PQGIFQWEKNYFHSQIPEDFIYNVALDNMNNIYVTGISFSTITDYDIATVKYSRTVGI
                     NQSQNYLPEKFFLRQNYPNPFNPSTVIGYDLNVSGFVSLKIYDILGKKITTLVNENQQ
                     AGTHEVLWDASDSPNGFYFYEMKVEKYSSVKKMLLIK"
     gene            16491..19697
                     /locus_tag="PLX80_03600"
     CDS             16491..19697
                     /locus_tag="PLX80_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQY19913.1"
                     /translation="MIEKIIDFSVNNRFIILIIYLGVIGYGVYSMINTPVDAIPDLSE
                     NQVIVWTEWMGRSPQIVEDQITYPLTTSLQGMPKVKAVRSQSMFGMSFIYVIFEDNAE
                     VYWARSRVLEKLSQVQSSLPEGVTPVIGPDGTGVGHVYWYHLVSDKYDLGELRALQDY
                     YLKLGLQSVEGVAEVASVGGFVKQYQIDADPNRLSAYGLGINELAMAVQRSNKDVGGR
                     NIESSDIEYFVRGKGYITDVSDIEEITLKSGANGIPVKIKDVATVQIGGDIRRGMLDL
                     NGEGEVVGGIIVMRYGENAQTVIDRVKEKLIELEKGLPEGIEIQTSYDRSDLISKAIS
                     TLRDAIIEEAIIVSLIVLVFLFHFKSALRILIEIPVAILISFILMRQFGITSNIMSLG
                     GLIISIGILVDASIVMVENAYRNIAIAQEKGEKIDYKEISAKSAKQVGRAIFFSEAII
                     IISFLPVFMLEGQEGKLFHPLAFTKTFAIAGSALITITLVPMLMTLFMKGKFFKEEQN
                     PVSRFFSKIYDPILRLALKYRKTTLGINVIALLITIPLIMNTGSEFMPPLDEGSLLFM
                     PTMLPNVSITEAKRVLQVQDAIIKSIPEVESVLGKVGRAETPTDPAPVSMIETIIILK
                     DKSEWREGITKAEIVKELNSKLQIPGVSNGWTQPIINRINMLSTGIRTDLGIKIFGDN
                     LDTLEKLAIKAENLVKNIPGAADVYAERTQGGSYIDIDINREAISRYGLNVGDIQDVI
                     EMAIGGENLTTTVEGRRRFPVRVRYMKDYRSDVEAIKNIWVTIPGSGGSNMPGQNNEM
                     TSGRQVPLGELVDINITSGPPMISSEDALLRSVVFLNVRDRDMGSFVNEAKEVLDKEL
                     SPSLPGGYYYTWSGQWENQIRAKKRLQIILPIVFLIIFVMLYFTFNNFLEAGLVMLSV
                     PFALIGGVYYMAILDLNFSVAIWVGFIALYGVAVETGVIMVLYLHEALDKKLIEKDGN
                     ISQQDLMDATENGAILRLRPKLMTVATALLGLIPVMWATGTGADLAKPLAVPLIGGII
                     TSAIHVLFVTPVIFVMIKEYYRKKGKLKKSEMSKFMVQ"
     gene            19892..20401
                     /locus_tag="PLX80_03605"
     CDS             19892..20401
                     /locus_tag="PLX80_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HQY19914.1"
                     /translation="MPRIAFYTFTILKEKYGHPQVQGFVDKIEDVFQQARDSVGFIEL
                     DNIRWGKYVSPKFFNEELHAAAPATLSLWKDIESVCAFAYKNNHGVALKLREEWIIKP
                     EWPTYVAWWIEDDKLPTREEACQRLEFIHDNGPTSYAFNFKKLFDENGKPVELDKQLL
                     YQLVRSNEK"
     gene            complement(20553..21293)
                     /locus_tag="PLX80_03610"
     CDS             complement(20553..21293)
                     /locus_tag="PLX80_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HQY19915.1"
                     /translation="MKNVILTLFLASFLFASCGEKKEGKEEAKKESKEEVTAKNDVMT
                     QELQQAKTPAKIIEVLKEGNAKFVKGDLTFTDYPKQIIKTSTGQFPSVVVLSCLDSRV
                     PVEKVFDLGIGDVFVARVAGNIVNPDILASIEYGCKVVGSKLVVVLGHTECGAVKAAI
                     DDVKLGNITELLEKIKPAVDSLSKFEGEKTSKNVKFVNEVTVENVDVAMDNIRKNSPI
                     LKEMEEKGEIDIIGGMYDVNTGAVTFID"
     gene            complement(21734..23146)
                     /locus_tag="PLX80_03615"
     CDS             complement(21734..23146)
                     /locus_tag="PLX80_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19916.1"
                     /translation="MENSQLIESLRTLDKNEIKEFGKFIRSPYFNNRSEVIRFFNYIK
                     KFFPEFKSNELKEEIIFKNVYPDKKYSSVMTRKLISLTLNLLLDFLTLEDFKENKNDY
                     NVKMLDMLRKRKLPALFQKRSRITGKNLDISTLDIYYYEQKLKFTSIQNGYFLNSDES
                     SFVDGLQNELNDLIEFFLTGVILQFIRLSEWSRQLNKKFDLKFYDEILKYMSADDERE
                     VTLYSMYFNMLMLLNTEDENYFKKLIISRDTLKAKLGKIDDYNVQIVSMQYCHKKVQK
                     GYPEYRKQMFEITKKLLAADLIPDGFIEPYFFTNIVRNASLLNEFDWLDNFLSEYKHR
                     LNPELSDEITNYSQAMIESAKGNFEKSLMHFSKIKIERSNMKLDMKNLLIIIYYELNF
                     IEELISLIDTNKHFLQRDRSVSDSYRQVSLLLMNFVSELIKIKSSNKKISTHGLRKEI
                     EKSDYFIFKEWLLKKAEELK"
     gene            23353..24921
                     /locus_tag="PLX80_03620"
     CDS             23353..24921
                     /locus_tag="PLX80_03620"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY19917.1"
                     /translation="MKKIIFYLCIVSSFAVGFRGGCLIHFVNFAIHNPLGIDDGFLYL
                     NSDAGMNENYYIASGEGGKIWRTTDPVNIPWDERVSNTTNDLTYVNDSKRSDTSVTYI
                     VGRSGTIVRSLDQGMTWTLHPSGVNNDLNGIDFIGSGIDQIIAVGDSGLVLRSSDGGE
                     TWSPVNSGVIKNLNSVYSLSSFITIIAGDEGTVLKSSDSGLNWVNQSLPDTTSDLNKI
                     SSMGSWFFGPILGMVGENGKLYSSTNYNFWNPVETGTTENLYDLQFKNSSSGYVTGEN
                     GTIQYTIDGGNQWFSEIFLSTITSERIRASIMLNDTTAVGVAGSDIITIHANETLLPV
                     ELASFNFNVQNNNVNLYWSTLSEINNSGFEVERKLCDEQHQQNWVRISFVEGNGSVNS
                     TSDYEYTDKGLNAGKYNYRLKQIDFNGNFEFHDLSGEAVIGIPDKFELSQNYPNPFNP
                     STVIGYSLSQSGFVSLKVYDLSGKEVYTLVNEIQTAGNYEVTLNGSNLSSGVYFYKMQ
                     AGEFISTKKMLLTK"
     gene            complement(25170..>26406)
                     /locus_tag="PLX80_03625"
     CDS             complement(25170..>26406)
                     /locus_tag="PLX80_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQY19918.1"
                     /translation="AAIDDIGGPKYYNHYPWGWTFAGNTPFKRWKRETYRGGVSDPFI
                     VHWPKGIKSKGEIREQYAHAIDMVPTVLEALDLEPPTVINGITQSPIQGNSFAHTFND
                     GKADSSHEVQYFEMMGHRSIYHNGWRAVCPWPGESFTEAGMGFGAPITADKLTELDAT
                     AWELYDLTKDPTEMNNLAKENKAKLIEMISQWYIEAGKYNVLPIDSRGVERLAEPRPQ
                     IALPRTKYTYYPGTQPVPSNASVNILNRTHTITVDAEIPEDGAEGVLMSMGGCDGGYT
                     LFIKDNKLQYGYNYVTAEYFYVKSTEDVPAGRHNFRFEFEVTGKPDFANGKGSPGKAQ
                     LYIDDKLVGESDFPYTIPFSIGLGASIAVGADPGSPTIPDYDPPFDFTGKIYKAIIDV
                     SGELIEDHEVALKNVMARQ"
BASE COUNT         8888 a         4213 c         4691 g         8614 t
ORIGIN      
        1 tctttaagtc atgtacaatt tacagatcaa taactgccgt gaaactaaac tatagaatta
       61 gttaacggaa tattagaaca aacatgtaaa aaactatttc agcgtgttaa tggaataaag
      121 attataaacg cttttgctgc catcagagtc ataaaactct cttgtaacaa ctttctgaag
      181 tttattattc tttttcatcc caagcttttt aagattattc tcagctattt tatttttctt
      241 atggacatat ccgacaatgt atttgaattt tttggaactg cttaagtgat ccaaaagcag
      301 tttcgatgat tcatatccaa ggtccagtcc gcggaatctt ttgtcaatgg ctgtagaaat
      361 ttcacaggca tcatcataat gtttccgcca tttataacag atatatccga cataatcatt
      421 tttatgctta atgacatacc atatctctac aggattagaa tactttgact taagccattt
      481 tctgatctga ggttttgtcc tgtaattaag gtattccggg ttcaggaatt caactagttc
      541 ttcatcattg aagatattat gcataagttc aaccagcttc tcatcagagc cgatgaatct
      601 tatcaaagaa cattttttcc cttttattat atttttaaac aatattaaaa agtaaaaatt
      661 aaaaaataat tatttatata tctttcggta cgactgttct gtaccatggt ttttttgtcc
      721 tgtcctttat ttcgtctata tgaatatgtt ttgcttccat atattctttt aatccatcca
      781 tgccaaactc tctcccaata ccgctttgtt tgtatccgcc gaatggtgct ttatcactta
      841 acaaatgata ttcatttatc cagactgttc cggctctgag tctgtcggca attttcaaag
      901 ctctttcatc atttgaagac cacactgcac ctgcaagacc aaatattgaa tcgtttgcgg
      961 ttgcgacagc ttcatcatca tctttaaatt tcataactac gagcacgggt ccgaatatct
     1021 cttcctgtgc gatcttcatt ttattattta catttacaaa aacagtaggt tctataaaaa
     1081 gtccgctgtc tccggattgt tttccgccgg taagtaactc agctccttct tcgatccctg
     1141 tttttatata tttcattact gtatcaaact gactttgatt tacaaccggt ccaacagtta
     1201 cagacttatc catcggatca ccgatcttca ttctttcaat tttttctttc attccggaaa
     1261 tgaatttatc atatatcttt tcatgtaaaa gtaatcttgt accgccttcg cagcattgtc
     1321 cctgatgata aaatgcagca tagatcgctc cgtcaatact catttccaga tccgcatcat
     1381 cgagaactat attagcagat ttaccgccac attcgagtgt aatttttttc agattaatag
     1441 aagcgttctg cataatgatc tttcctactt ctgtagatcc ggtaaatcca actttatcaa
     1501 cgtcaggatt ttttaccagc tcagtgccga tctcacttcc gcttcccgtt ataatattta
     1561 ttactccttt aggaatgtca gtttcgctta cgagtcttgc aagttcgcag gcagtgactg
     1621 aagtttccat cgcaggcttt aggattactg tgttgcctgc agcaagtgat ggaccgagtt
     1681 tccagatagc catttttaaa gggaaattcc atggagttat cagagcacat acaccaatgg
     1741 gttcatatct gataatattt ttgctaactc cttcttttga caatccggat atttcctcat
     1801 tcagatctat tgcagcagct ttggaaaaat agttcagact ttttgcagaa aggaaaacat
     1861 cttcaccggc ttttctgatt gttgatcctg aatcttttac ttcaagctca gtcagctttt
     1921 cagcattttc attaatttta tcaactaact gttttaataa tcttgatctt tcttcgcccg
     1981 gaagtccgct ccagataccg ctgtcaaatg catcccttgc cgctttgact gctttttttg
     2041 tatcggaaat tcctgccttt gaaatttcgg caattacttc gccattggat ggatctgtag
     2101 ctttaaatgt ttgcttatca tcagaatcac aaaactctcc attaataaat aaccggtaat
     2161 tcaacatata ttataaactc tctttgattt aatatttaaa aaatataaaa cttttctctc
     2221 ctgaaactaa atttaccaca aaaatctaaa taaaaaattt ggtaattcaa aactattaac
     2281 tattaactac taactactaa ctactaacta ctaactacta actattacat attaactact
     2341 aactatacta actattaact attaactatt aactaactac atatacaaat aaggcttcca
     2401 cccctcatca attctccctg caaagttttt tataaacgta atatgagatg gtctctttgg
     2461 aatattcagc aatgtgaatt ttgcctcatc cggagtccgg ttcccttttt tattattaca
     2521 tttaatgcat gcagttgtaa gattttccca ggagtcttcg ccgcctctgg attttggcag
     2581 tacatgatcc acagtaagat tggaagttac accgcagtac tgacatttat gattatctct
     2641 tctcaaaata tttttacgtg aaagtatgat ctttttaaat ggtactctca caaaaaaaac
     2701 aagtcttact atacttggat attcaaagct tctgtttact gtatatattt tttttgcttc
     2761 aagacaataa atagattctg ctttgcctaa atatatcaga atgatcgctc tgcgaacatt
     2821 gcaaatagta agaggttcat aattctgatt aagaaccagt actttgccgt ttagattact
     2881 gtatcggcgg tttgctgtat ttttagtatc ctgttcgtgg aagacaacct ccttttgttt
     2941 agggcttagc ttagatagac aaattatgaa tattaatatg tatagtcaat tttattattg
     3001 agttgcaagc aaatatagta aaaataaatt tgtaaaagaa agaataagaa tttattgaaa
     3061 tatttattta atctgtttga aaatttaaat taacaattgc attaaaactc atgtttgaaa
     3121 agatgtaaga taattaaaaa aattctaaat ttagaattta gtgttaaagt ttaaattaat
     3181 gacataacac attttaaaag ttccgattaa atatgatcag tcaaaagaaa ttaactgata
     3241 cataccattc ttaactaaat tctttaagtg catccgtaag atctgtaata tcttttttgt
     3301 tattaaataa atgcgccgac agtctcagga aaccttctct taatgctata aatatttttt
     3361 ttgattcaag atatttctgt acttcatcat tccttttatt atctttatag ctgaatataa
     3421 gaatattaga cctgtggaca ggatccggat ctgaatcaat aataaaatct gaatcatcca
     3481 tttcatttat aaaaatgtcc tgaagttttt gaatgtgtcc gaatatattt ttcacaccaa
     3541 gtttcaaaaa caaatcaatt gatgagatca gcccgatcat acccagtgta tttggagtgg
     3601 aattttcaaa agcgttaccg tcttttttaa aattcagttt gtagtcgaga aaatttttaa
     3661 agtcaaattc aatactcgaa gttgcagcgt acgtcggatc cacattatcc cttatcctgt
     3721 ctgagataaa ggcaaagcca ataccggccg gagccatcat ccatttctgc gaacctgcag
     3781 ccatgaaatc tattccggat tcttttacat ccacaggaca tacacccatt ccctgaatcg
     3841 catctactac aaaataacaa ttgttctctc tgcatatttt tccaactgca ttaagatcat
     3901 ttttgaatcc gagaaactca accgaactta tagtaaggac tctgacatta ttttcgcgta
     3961 tggttttttc aatatcaccg atctttattt taccgtttac agaaggtatc aaaatatttt
     4021 caatcccacg gagcttttgc tgattcatcc agggatatgc aactgcagga aattccgagt
     4081 ctgcgtaagc tgctttttca ccgcgttcca gtttaattcc gttggcaaag atatttaatc
     4141 catgggtagt gcttcctata ataataattt cgtttttatc agcgtttatc agatcagcgg
     4201 ctctggattt caaagtctct gacagcttca tattaatcag actaagattt ttatttgtga
     4261 aagtaaattc atctatgaat tccatcagcc ttcttctggt ctccagtgaa tactgtgaat
     4321 aatgtgctga gtcaagatag acacaggatt ctgttacagg aaaaagattt cttgcttctt
     4381 cgatgctgtt tatttcatct aataaggata ttgattcaga tgttaagctg ttcattaaaa
     4441 tttatttttt atttttttta gattttttag taccggtctt tttagtttcc tttggttctt
     4501 ctaagttact ctcatacatt tttttgtaag attcataata gaacattatg ctgtttacat
     4561 aattgtaagg ttctttccag ttgtcgagag ttccatatgg aggtctttga ctttcaggcc
     4621 agacaagcgc atgaatagaa tcttcttttg ctgctaaaaa cggatatgct tcctttacat
     4681 tttcccactg cttatgatcc ttattaaagt aagcagtaat tgtcatcgca tctatcactc
     4741 ttccgagacc tgcattgtaa gctgccagtg cgaatttata tttctcatct ccataatcac
     4801 gaaactgaga taccagagag gcataatagt aaattccggc tatcagatta tttaccggag
     4861 tctgcgtttc ttcaagctta agtgtattct gcagaccttc acctgtcctg ggcatcagtt
     4921 gcatcagacc gaatgctccg gcatgagacc ttgcgttagc attgaaataa gattcctgtc
     4981 tgatgatagc aagtactatg cgccagtcca gattgtaaaa attactttct cttgtaatta
     5041 cttcaccgag ttcttttact aaattttcta cattaccgag tttaaattct ttactgacca
     5101 gattcacttt ctcagagatc cgttccagat cttttacgtt cagatttttt gcctgctgtt
     5161 tgaaattagc atttgatgca aacagatcaa acagtgtgtt ccagtcttta aacatatcct
     5221 caatatacat gttactgctg aaagattttc ttgaaataaa attattaaga ccttcaattt
     5281 tttcgtgttt atccggtgat ggagaaatag catcattgtt atttatatta ttcacaccgg
     5341 acctgatact atatgagtca gaataaattt gtgattcatt atatgaataa aataaaagaa
     5401 ctataatgag cataaatgga agcagaatca gaatgaattt tttaaagttt tctgaaagat
     5461 gatatgtatg agtattactg ataattggta ttatgaattt gaattcagaa taatatttta
     5521 ttaaaaaaat cttattaaaa tataagggat taaatattgt aaatttaacc atttaataaa
     5581 tgtttaacaa tttaagaata atatcggcaa aaactcttct atattaaaat aaattcttta
     5641 aacagaattg ttggtgaaaa ttaataaagc atccataacc aagatccaaa ataaaaataa
     5701 ttgcttctct tatttttacc tgagggatgg cagagattta atttatcgtt taacctgtcc
     5761 atcttgatta catcagggga attattatga ccttcaaata ctttaactaa aattatccat
     5821 tataatttta gatcattttt tatatattcc atagtgataa aaaccatttc aaaatatttt
     5881 tcagttttac tgattcttat cttagtaaac tccaatttct cttttgcatt ttctcaaatg
     5941 ttatgtgaaa tgagtataga gccgacagtc tgtaaatgtg aagaaaataa tatgtcagac
     6001 gaagtgcctg cggatgtttc taaaatgagc tgttgtgaca ataatattct tgagatcaac
     6061 aattccaaca cacttgtaaa aaataatact gattatacaa atttaaattc aatacaacta
     6121 ttaatttctt tgttgcctca cattgaaatt acaaaaacat ttgcagacca tttagttttt
     6181 cagaaacatc ataaaccacc ttcagatatt cccattttaa tctcttcgtt cctcatttag
     6241 tattttcatt taaagaaatt ttgtttacgg caaattattt gtctgccgtt taaattattt
     6301 tacttttttt aaagtaaatt taaatgaaaa atgaaatatt taataatcca tttaatatta
     6361 ttacccggtt tgcttttcgg acaggatgtt ccgcaaagta attatccaaa aaccggtctc
     6421 gaagatatca taaaatattc cctcgagacc aattccggcc ttgctccttt agaatatgaa
     6481 agcaaaatca tgtcagcaag aatagacaga actaattttc agccttcacc ttcttttcag
     6541 tttatgctgg actttttacc tgtggacctg agcaatgcag gagaatatat tggcatttat
     6601 tcacagccgc tcagactttt tggaaagctt gacgcagaag aaaatcttgc gaaagtaaat
     6661 tctcaaagac cggaaattga aaagactgca ttggaaaacg aactgatcag atcagtaaag
     6721 gaaaattatt ttatgctttc agtgaatgag cgactgcttt catttaacaa tgagttcaag
     6781 gaaattatga aagctgttac ttccacactc gaaataaaat attcatcagg aaagggaagt
     6841 cagtttgaca ttctgaaaag caataatgaa tttcagaaac ttcttctgga agagattgag
     6901 ctggcaggta ataaaaaaat atttataaat aatctgagga ctctttcaaa tctggatctg
     6961 ccttatgact tctccacaaa aaacattgag atacttttga atattcctac aattgatatg
     7021 gacacttcaa aacttatttc tgaaatgaaa ttaaataata cggattttaa atttattgat
     7081 cagatgcttc tggtgaatca gactgaacaa aacataatag atctggagag aaaacctgat
     7141 ataaatttaa tgacaggtta taaatataag tctgataaaa aagcaagttt tcttatgttt
     7201 tctgtaatgg tagaacttcc ctttatgcca tggaatgaga aaagaataga cgcagagatt
     7261 caggaaaaaa tttacagaga taaaaagata aattcggaat caaaatccat tgagctgaat
     7321 cttaaaaatg aattaaaaaa taatattgta aaaataaatt cttcactgga aaagatcaaa
     7381 tatctgaacg aagttcttat ccctcagaca gaacagacat tcaggtcatc attgttatcc
     7441 tatgagactg attcgaatca attcattgat ctgctggata cctacagatc tctcagggaa
     7501 aacagcaaaa tgcttgctga agaagaaact aattatttga tattgatcag tagtttagaa
     7561 aaattaattg gcaaacaaat cttaacaatt aactgacatg aataaaacat taataatatt
     7621 gatcacagtt ttgattacca caggtctttt atttggggct tataaaatat tttatgatca
     7681 gggaaaaact gaagaattaa ttgctgcaga tgaattatac atctgcccta tgcatcccca
     7741 gatacaaagt gatcatccgg gagtatgtcc tatttgtaat atggatctgg ttttaaaaga
     7801 aaaaacaaat actgaacttg aatttgattc tttaaaaaat ttaaataaag aaattagtga
     7861 tttagtctta tctcctgctc agcagatact tgcaaatgta cagactgaaa aagtaattgt
     7921 aaaagaattt aaaagcagaa agagttttaa cgggtatgta aaatcggctg acaataacat
     7981 gagatttatt tcaactcctg tttcaggaaa aattgttaaa atgtatataa attatgaagg
     8041 tcagactgtc agtaaaggac agagggtatt tgatatttat tctccggaaa tttattctac
     8101 gcaaaaggaa tatctgctcg ctttaaaaaa ttatgaaagt gcaaaaagca gcggtaacac
     8161 tctagtgata gaccaggcgg aaagtctgat aaacgcaagc aagatcagaa tgagcttatg
     8221 ggaaattaca aatgagcaga tcagtgaact ggagcagtcc ggagaagtta aagattttat
     8281 ttctgtctat tcgaagtaca gcggtgtggt tacgaaaaaa ctccaaaacg aaggtaggtg
     8341 ggcgatggca ggtgaaaata ttctggatat aacagatctt tcgacagtat ggataatagc
     8401 aaatgttcct gaatcagatt tgaaaaatat ttcaatgggt cagaatggaa tgatcacgtc
     8461 tgtagcttat cccggtgaag tttttaatgc aaaggttaat tttataagtc ctgtatttaa
     8521 tgcagattcc cgttcattgg aagtaaggtt tgacgtaact aacagaaatt acaaattaaa
     8581 acctgatatg tatgtagatg tagaaatagg atcttcagat tatcagtgga atatagtagt
     8641 accaaaaaat gctgtattga gaaccggtaa gatggacatg gtatatatta agaaaagtga
     8701 aaatttattc tcaccaaaaa tggtaacagt cggaggagtt tcaaacggat tttatcttat
     8761 aacgtcagga ctttctgaag gtgaagaagt ggttaccagt gcagggttct tgcttgacag
     8821 cgaaagtcag atcaggtccg gttctaatat gaataatatg gaaggaatgg aaatggaagt
     8881 aaaaggtgat cctgaattca acaaagatca ggatgttatg aaagatatac aggaacataa
     8941 acactaataa aaaaatatta caaactaaaa attctgcaga tgcagagaag gagtaaaaaa
     9001 tgaaatacat taattttaaa aaaacaacag taatgatcat tagtatattt ttagttacgg
     9061 cattcatata cggatgcggt gaagataaga ccggtgaagt tactgaaaag gcaaaggaag
     9121 aaaaaacaac agaatcaaaa atggaatctg aatcacatga tatgaataat gcaaacatgg
     9181 aacatgcaga tatcaagcta tcatcaatgc agtgcggaat gtgtaagaaa actattgaga
     9241 cagctgtgaa aaatacggac gggattcaaa gtataattgt aaataaggat gaaaaagtag
     9301 cgcatgtaaa ttttgataaa agtaaaaccg atcttaaaaa tatcgaagca actattacag
     9361 cggcagggta tgatgcaaat gataagaaag cagattctag agcatatgaa aatctggatg
     9421 actgctgtaa attacccaat gatcaaaaaa gtaaaaaata atttcgaaac aaaataacaa
     9481 taatttaaaa actaaaatta aaaaaaatga aatcagcaat ctcaaaaaac ttaaaagtaa
     9541 tatttgcaat tttttttatg gttacattaa ttcctatgtt atcgaaagct gataataaag
     9601 gtaatgaaaa aacagttgag atcactttac cttcgatcca gtgcggtatg tgtgtaaaga
     9661 ctgtagaaaa ggcattgggt aatttggatg gtgtaataga ttcaaaagca gatcttgata
     9721 ataaatccgt gacagttatc tatgatgatt caaaagtaag tgtggagaat ttagaagcag
     9781 tgatcactgc atcaggatat gatgcaaata aaatgccggc aaatgaagat gcatacaatg
     9841 aattaaatgc atgctgcaaa aaaccaaaat agtatgaaat atattttaat tctcatattt
     9901 tttgtaataa tcattctcat tgcttaccgg aagtttaaaa aggtaagcaa tgggaaagat
     9961 tgctgtaagt aaaatacatt aataaagtga aatgactaaa gtacaattta aaaaaatgaa
    10021 gaaagatatt ttataaagta ataaataatc aggaaatttt tataaacagt aaaatctgaa
    10081 aaagaatatg cttcttcaaa taaaaaatat ggtctgcgat aggtgtataa tggtcgttaa
    10141 aaatgagtta gagaaacttg gttatatggc taatcacata gttttgggtg aagcaacaat
    10201 caatgaagtt ctgaatgatc aagagaaagc aataatagaa aataatttaa aagaatttgg
    10261 ttttgaatta attgatgaca agaacagcag cctaattgaa aaagtcaaaa atctaataat
    10321 tgatctggta cataatcatg ataatgagtt aaaaataaat ttgtcagaat atttagaatc
    10381 taatattcat aaagattata catatttaag taatcttttt tcagaatctc agggcattac
    10441 gattgaaaaa tattatatca gacaaaagat cgaacgtgta aaagaactta tagtttatga
    10501 cgaattgact ttgagtgaaa tttctttcaa tttgggttac agtagtgttg catatttatc
    10561 aagtcagttt aaaaaaatca caggtctgac tcccggtcat ttcaaaaaaa taaaatccat
    10621 aaaaagaaaa tctttagatg aattgtgaat ctgtgaatat cataaatttt tataaaaata
    10681 acataatagt tatgcatttt attattacta ttttggaata gaaagaaact aaacaattaa
    10741 catttttaaa caaaaaaaac aaaattatga aaacaataaa gaaagtaaca ctgacatttg
    10801 cagcaataac tttaattgct gtattttcat cctgtcagtc aagcccggac gtaaaagaag
    10861 ttttgtcaaa accggaaaca agaaatatga tcatggactc aattgctaat aacagcacca
    10921 tgtctatgga aatgatggag tcaatgatga aaagcaaaag cggaaatatg atgatgcagg
    10981 gtgacggaaa aatgatggga aaaatgatgg gtatgatgat ggaaaatcac gatggtatga
    11041 tgaagatgat gaaagagaat cctgctatga tggaaagtat gatgagtaaa atgatggaaa
    11101 tgtgtaaaag cgattcaacc atgatgtcag gcatgtgtaa aactatgatg ggtgataagc
    11161 aaatgatggg tatgatgcag gacatgatga aagatgataa aagtatgaat atgacaggta
    11221 gtgagaaaaa gatggataaa atgaacgata ataatatgaa tggaatggat cataataaga
    11281 ctgaaaagaa ataaatctaa acaataattt atttttccca aaaagatttt aaataaaaaa
    11341 atggatcact ctaaaatgga tcacagcagt atcccaatgg gaatggaggg tcacgatcat
    11401 catcagatga tgatcaaaga tttcaaattg agattttatg taagtgtcat acttacaatt
    11461 cctgttctga tattgtctga aatgatacag ggtttttttg gatttcatat tcagtttccg
    11521 ggaagtcaat atttggtact tatactatcc acagttattt atttctgggg cggcaaacca
    11581 tttctaacag gatttatcag cgagatgaaa aaccgttctc ccggaatgat gactctgatc
    11641 gcaatggcaa tttcagttgc atacttttac agtgctgcaa ctgtctttgg actgagtgga
    11701 atggatttct tctgggagct tgcaaccttg atcgatatta tgctgcttgg tcattggctt
    11761 gaaatgaaat ctgtactcgg cgcttcaaaa gctcttcagc tgctggtaag tatgatgcct
    11821 tctgaagcac acagggtaaa tcctgacgga cagactgaag atgtaaagtt agacatgatt
    11881 aaaaaaggtg atactattct tatcaagccc ggagagaaag ttccggctga cggtataatc
    11941 accgaaggca gcagctatct aaatgaatcc atgctgaccg gcgaatctaa acctgttaag
    12001 aaagaatcag gcaataaagt gattggcggt tctatcaacg ggaatggttc tttaaaaata
    12061 aaagtcgaac atacaggtaa ggaaagttat ctgaataaag taataacact tgtcgaagaa
    12121 gcgcaaaagt caaaatctaa aatgcagaat ctttctgacc gcgctgcgaa atggctgacg
    12181 tacattgcat tggcagttgg attcgggaca cttgcagtct ggcttttctt aggttttcca
    12241 tttgtgtacg cgcttgaaag gatggttact gtcatggtga tcacatgtcc acatgcgctc
    12301 ggacttgcaa ttccattggt cgtcgccata tcaacagcaa tttccgcgca gaacggatta
    12361 ctcattcgta acagaactgc atttgaagaa tccagaaaga tcactgtgat tttatttgat
    12421 aagactggga ctctgactac cggagtgttt ggagtgaata gatttgaaag tatatctgat
    12481 atatcatccg aagaaatgct caggctttca agtgctttgg agcaaacatc cgaacatcca
    12541 atagctgtaa gtataattga aaaagttaaa gagcttgata ttaaaattcc ggctgctgag
    12601 aattttaacg ccattaccgg aaaaggagta gaagctacag tcgaaggtaa acagatcaag
    12661 gtcgtcagtc ctggttatct taaggaaatg aatattacaa ttcctgacaa agcatttaca
    12721 aataatgcag agacagtcgt atttgttttg atcaatgaca gactcacagg atatatttca
    12781 ctgtcagatg tcattcgtcc ggaaagcagg gatgctataa atattttaaa aaagaacggg
    12841 atcaaagtca tgatggctac aggtgataat gaaacagttg ctaagtcagt ttcagatgaa
    12901 cttggtcttg acggatattt tgcggaagta ttgccgcatg agaaagttca gatagtaaaa
    12961 gaccttcaga gcaaaggaga atttgtagca atgtcaggtg acggagtaaa tgatgcgccg
    13021 gcacttgctc aggctgatgt gggaattgct gtcggatccg gaactgatgt agctgctgaa
    13081 actgctgaca ttgtacttgt aaacagtaat cctaaagaca ttgcaaaatt aatactgttt
    13141 ggaaaagcga catacaaaaa aatgatacag aatatcgtct gggctacagg ttataatact
    13201 tttgcaatcc ctcttgccgc aggtgtattt tattcttcgg gatttgtatt gggacctgca
    13261 gtaggagctg tcttcatgag tcttagtact ataattgttg ccataaatgc gcagttattg
    13321 aaaaagcaaa tgaagcagtg attgaaactc aaatttaaat caaactaaaa ttttttatta
    13381 acattaataa taaaattaaa gaagatatgt acaagaaatt tattttaatg ctttcattat
    13441 catttgtaat aatgtatgcc gtcatgttct taaatattga cgatctgagt catatttatt
    13501 taagcttaac acggttatat atgagtctgt taatggtatc tcccatggca atattaatgc
    13561 ttttattaat gccaaaaatg tatactgata aaaagctcaa tgctattatc ttaattgtaa
    13621 gcgtagtagt ttttatcatt tccctgacat ttctgagaat gcagacaatt attggagata
    13681 agcagtatat gctggcaatg atccctcacc actcgtcagc cattttgacc agtcagaatg
    13741 ctgatcttca ggatcctgaa gtaaaacaac tggctgaaga tattattaaa gctcaggaaa
    13801 aagaaattac tcaaatgaaa aacatacttg aaagaatgga aaagtagtat caatagattt
    13861 aatcatcata aaaataaata aaattatata tgactcacaa attcaaagtc acaggaatga
    13921 catgcagcag ttgcgcaggc aaagttcaaa agacattgtc aaaagtaaat ggagtggaga
    13981 gtgtaacggt cacacttgat cctcctgaag ctgagataaa aatgcatcat cacattgaag
    14041 aaagcacttt taattcagct ttgaaaaata tcggtgatta cagactcggt gatgaagtat
    14101 cttctgatca tcttaaggct atgacagaga ccggagagga agtttcaaaa ttaaagactt
    14161 acaaaccttt aataatagtg ttcctctata ttatacttgg ggtaacaata cttgaaataa
    14221 gatcaggcag ttttgattgg atgaatgcaa tgaataattt catggcagga ttctttctgg
    14281 ttttctcttt tttcaaaatg cttgatctga gtaactttgc aatgtcatac agctcttacg
    14341 atataattgc aaaaaaatgg tacggttatg gtttcgttta tccgtttata gaacttacgc
    14401 tcggtgtatt gtatttactt cagctcaatc cggtttttac aaatatggcg acgattattg
    14461 taatgggaat cagcagtgtt ggagtaattc agagtgttat gcaaaaaagg actattcagt
    14521 gcgcatgcct tggtgctgtt tttaatttac ctatgagtac gatcactatt attgaagatc
    14581 tgctgatggt tgcaatggct cttgttatgt taatgtattt gatctaaaaa tagctttata
    14641 aatacttttt aaattgaaaa ttctaatatt gacagttaca gaaaatgcaa aattatttaa
    14701 aagaaaatga aatgattttt tattctgaat ccaaaattca gattattatt atgattctaa
    14761 attattaagt gccattttta aaataaatat tccaatgaaa ttaaaataca ttctgctgtt
    14821 tttatttgca ctgattttaa aaagcggttt cactcagccg gttgaagagt ggactcaaag
    14881 atataatggg tctgcaaatt catttgacat agtatcaaaa attctggttg ataatgacaa
    14941 taatgtttac atttttggca gcggcaatca aaccggttca gcaacggatt ttttaataat
    15001 taaatacagt ccggatggaa acatactctg gactgaatta ttcaacggca gtggaaacag
    15061 tttcgatcat attaactctg cttgttttga taattcagga aatatttatg taaccggtta
    15121 tactacagac tttgaatccc ggaatctcat tactactgca aaatttgata catcaggaaa
    15181 ttttatctgg tcaaatgtat tttcaaagca aggttatatt attggtgacg gtaaagatat
    15241 tattgtagat aattcagata atatattagt ctgcggttct ataaaaagag aaattggttt
    15301 ctttgatatt gaagttataa aatattctcc tgaaggtgat gaaatctggt caaatacttt
    15361 taatggtgaa ggtaacggtc atgataatcc cgttactttg aaaaatgata atttggataa
    15421 tattattatt gccggtacat caaaatctct gtctactgat agtgatatga taattataaa
    15481 atatgattca tcttcaaatc tgatttggaa aaaaacattt aacggaacag cagatctggg
    15541 tgacgtactt acttcaatgg ttctggatac agagagtaat atttattttt gcggaaataa
    15601 atttaacact cagagttcat atgattattt ttatgctaag cttgatcaaa acggaaatat
    15661 aatttggtct ggaagttata acggaattgg taacagtatt gatattccgt cttcaataat
    15721 tcttgattcg tttaacaatg tatatatcac aggattcagc agagcaggta tggatttagg
    15781 cagtgaagat tttctgacga tcaaacttga tcaggatgga aataccggct gggtaaggaa
    15841 tttcgacggg attgcaaacg gaatggatca ggcaaattct attgcagtag ataactacgg
    15901 aaatgtttat gtcggtggcg gatctgatac gggtaatatt catcttgttt attcactgat
    15961 caaatatgat cctcaaggca tcttccagtg ggaaaaaaat tattttcatt cacaaatacc
    16021 ggaagacttt atttataatg tagcactgga taatatgaat aatatatatg ttaccggaat
    16081 aagttttagt actattactg attatgatat cgcaacagta aaatattcca ggactgtggg
    16141 tataaatcaa tctcaaaatt atttaccgga aaagtttttc ctccggcaaa attaccccaa
    16201 tccattcaat ccttcgactg ttataggata tgatctaaat gtcagtggtt ttgtctctct
    16261 gaaaatttat gacattcttg gaaaaaaaat tacaactctg gtaaatgaaa atcaacaggc
    16321 aggtactcat gaagttttat gggatgcatc ggattcacca aatggctttt atttttatga
    16381 aatgaaagtt gaaaaatatt catctgtaaa aaaaatgttg ttaattaaat aaggactttt
    16441 tgaattgttt ccctgtttgt gtttcatact attaaaaact tagtaaggat atgattgaaa
    16501 aaataataga tttttcggtt aacaaccgat ttataattct cataatttat ctgggagtaa
    16561 ttggatacgg agtatattca atgataaata ctccggtcga tgcaatacct gatctgtcgg
    16621 aaaatcaggt tatcgtctgg acagaatgga tgggaagaag tccgcagata gtggaagatc
    16681 agattacata cccgcttact acatcacttc agggaatgcc aaaagtgaaa gctgtgagaa
    16741 gtcagtcaat gttcgggatg agttttattt atgtaatatt tgaagacaat gctgaagttt
    16801 actgggctcg cagccgtgta cttgagaaac tatcacaggt acagtcttca ctgccggaag
    16861 gcgtgactcc ggtaattggt cctgatggaa ctggagtcgg acacgtatac tggtatcatc
    16921 ttgtcagtga taaatacgat cttggagagc ttcgcgcttt acaggattat tatcttaagc
    16981 ttggtcttca gtcagtagag ggagttgcag aagttgcgtc agtcggtgga tttgtaaaac
    17041 aatatcagat agacgcggat ccgaaccggc tttcagctta cggacttggg atcaatgaac
    17101 ttgcaatggc agtccagaga agtaataaag atgtaggagg aaggaatata gaatcttccg
    17161 acatagaata ttttgtgaga ggaaaaggat acatcactga tgtcagtgat atagaagaga
    17221 taacattaaa gtccggagca aacggaatac cggttaagat aaaagatgta gctacggttc
    17281 agatcggcgg tgacataaga aggggaatgc ttgatcttaa cggagaaggc gaagtggtag
    17341 gcggaataat agtgatgcgg tatggtgaaa atgctcagac tgtaattgac agagtaaaag
    17401 aaaaattaat tgaattagag aaaggacttc ctgagggaat tgagatacaa acatcttatg
    17461 acagaagcga tctcatatca aaagcaatta gtacattacg tgatgcaatt attgaagagg
    17521 cgatcattgt cagtctgatc gtactagtat ttctttttca tttcaagagc gcattaagaa
    17581 tattgattga aatcccggtt gcaattttga tctcgttcat attgatgcgt cagtttggta
    17641 tcacatcaaa catcatgagc cttggaggtc tcattatttc gattggcatt cttgtggatg
    17701 cgtccattgt aatggttgag aatgcataca gaaatattgc catcgcacag gagaagggtg
    17761 agaaaataga ttataaagaa atttcagcaa aatcagccaa gcaggtaggc agggcgatat
    17821 tcttttcaga ggcaataatc ataatttctt ttttaccggt atttatgctg gagggacagg
    17881 aagggaaatt atttcatccg ttagctttta caaaaacatt tgcgatagcg ggttcggctc
    17941 tgataacgat tacacttgtt ccaatgctga tgacattatt tatgaaaggt aagtttttta
    18001 aagaagaaca aaatcccgtt tcgagatttt ttagtaagat ttatgatcct atattaagac
    18061 ttgcattaaa atacaggaag acgacactgg gaataaatgt aatagcactg ctgatcacga
    18121 tacctttaat aatgaataca ggaagtgaat tcatgcctcc tttggatgaa ggcagtctgt
    18181 tattcatgcc gacaatgctt ccgaatgttt cgattacaga agcgaaaaga gtattacagg
    18241 ttcaggatgc aataataaag agtattcctg aagtggaatc agtacttgga aaagtcggaa
    18301 gagcagagac tcctacagat cctgctcctg taagcatgat agagacgata attatattaa
    18361 aagataaatc cgaatggcgt gaaggaatta ccaaagctga aattgtaaaa gagcttaatt
    18421 caaaactcca gatacccggt gtcagcaacg gatggactca gccgatcatt aacagaataa
    18481 atatgctctc gacggggatc aggactgatc ttggaataaa aatattcgga gacaatcttg
    18541 acactctgga aaaacttgct atcaaagctg agaaccttgt gaagaatatt ccgggagcgg
    18601 cggatgtata tgcagaaagg actcagggtg gctcttacat tgacattgat atcaaccgtg
    18661 aagctatatc cagatacggt ctgaatgtag gagacattca ggatgtaatc gaaatggcga
    18721 tcggaggaga gaatttaact acgacagtag aaggtagaag aagattcccg gtaagagtca
    18781 gatacatgaa agattacaga tcggatgtgg aagctataaa aaatatatgg gttacaattc
    18841 ccgggagcgg aggaagtaac atgcccggac agaacaatga aatgacttcg ggaaggcaag
    18901 ttccgcttgg tgaattggta gatataaata ttacatccgg tccgccgatg atctcatctg
    18961 aagatgcatt gctcagatcg gttgtatttc tgaatgtaag agacagggat atgggaagtt
    19021 ttgtaaatga agcaaaggaa gttcttgata aagaacttag tccgtcacta cccggcggat
    19081 attattatac ctggagcgga caatgggaaa atcagatacg cgctaagaaa aggctccaga
    19141 taattttacc gatagttttt ctgataatat ttgtaatgct ttattttacg tttaataatt
    19201 ttcttgaagc gggtcttgta atgttgtctg ttccgtttgc gctgatcggc ggagtttatt
    19261 acatggcgat acttgatctg aatttttcgg tggcaatatg ggtaggattc attgctcttt
    19321 acggagtagc cgttgagaca ggagtcatca tggttttata tcttcatgaa gcgcttgata
    19381 aaaaattgat cgagaaagac ggaaacatct cacagcagga tctgatggat gctactgaaa
    19441 acggtgcgat actcagactt cgtccaaagc tgatgactgt tgcaactgcg ctgctcggtc
    19501 tgatacccgt aatgtgggcg accggaaccg gagctgatct tgcaaagcct cttgctgttc
    19561 cgctgatagg cggaataatt acttctgcta tacatgtatt atttgtaaca ccggtaattt
    19621 ttgttatgat aaaagaatat tacagaaaaa aagggaagct taagaagtct gagatgtcga
    19681 agtttatggt acaataaaca gcaattttca tttttcaatt ttcaacgtat cattcagctt
    19741 agtaaatttt tactttacag ataaattttc tttcttgata aattgacatg caacggattt
    19801 aaatttattg ggagtatata aattaattgt taatactctt ttatattatt attattcgta
    19861 aaattattaa aaattatttt tcaaatttaa tatgccaaga atagcatttt atacttttac
    19921 aattctaaag gaaaaatacg gtcatcctca ggtacaggga ttcgttgata aaattgaaga
    19981 tgtgtttcag caggcaagag actccgtagg atttatagaa ttggacaata tcagatgggg
    20041 aaaatatgtt tcaccaaaat tttttaatga ggaacttcat gcagccgctc ctgcaacatt
    20101 aagtttatgg aaggatatag aatcagtctg cgcatttgct tataaaaata atcacggagt
    20161 tgctttaaaa cttcgtgagg aatggattat caaaccggaa tggccgacat atgtcgcctg
    20221 gtggatagaa gatgataaat tgcctacaag agaagaggca tgtcaaaggc ttgaatttat
    20281 acatgataac ggaccgacat cttacgcatt taattttaaa aagctgtttg atgaaaacgg
    20341 aaagcctgtt gaactggaca aacaactgct ttatcaactt gtaagatcaa atgaaaaata
    20401 aattattttg tacagattat tattttacta aagttataaa ttttaaaagt aaaatttgtt
    20461 aataaaaaaa cagccggctg tatattcatg ccggatgttt ttataaatat cctgaaaaaa
    20521 ataaaaatct gttttagaaa attttatatt agttaatcta taaaagtaac agcacctgta
    20581 ttcacatcat acatcccgcc aataatatcg atctcacctt tttcttccat ttcttttaag
    20641 atcggactgt tctttctgat gttgtccata gccacatcaa cattctcaac ggttacttca
    20701 tttacaaatt ttacattttt agaagttttt tcaccttcaa attttgacag actatcgact
    20761 gcaggtttta tcttttctaa aagttcagtt atgtttccaa gcttgacatc gtcaattgca
    20821 gctttcacag cgccgcattc agtatggccg agcacgacta ccagtttcga accgactact
    20881 ttgcagccgt attcaatgct tgccagaatg tcaggattga cgatattgcc tgcaactctg
    20941 gcaacaaaaa catcacctat ccccagatca aaaacctttt ctacaggaac tctcgaatca
    21001 agacaggata aaaccacaac agatggaaac tgcccggtgg aagtctttat tatttgcttt
    21061 ggataatctg taaatgttaa atctcctttt acgaattttg cattaccttc ttttaaaact
    21121 tcaattatct ttgccggtgt ttttgcctgc tgaagttctt gtgtcattac atcatttttt
    21181 gcggttactt cttcttttga ttcttttttt gcttcttctt ttccttcttt cttttctccg
    21241 caggatgcaa agagaaatga ggccaggaac aatgttagaa ttacattttt catttttatt
    21301 tattttgatt attgaatatg tacctgcatt cccgcctgta caactggtat ttttctaaag
    21361 gcttaaacga gtttgtaaaa ataatatttt tattttatcc gatagtaaac ccgtatttat
    21421 tcctgtaaac tgttgtaagt cacagtcaca aaataaagct gataacgaaa attttattaa
    21481 tgtaaataat tcagagattt tactggtgat tactttataa aagtcagaat gtatattcct
    21541 tttcctgatt aaaataatat tctttgaaat tagctgtaaa cttaaaaaat tatatttttt
    21601 taatttgaaa gaaataactt tctgattaat tttataatat attctggagg tgtttaaata
    21661 tgttgtattt acattttaat ttcaatcata aattttatat ctgatgactg aaacaggatc
    21721 tttattatat tatttatttt aactcttctg cttttttaag cagccattct ttaaaaataa
    21781 aataatctga tttttctatt tcttttctca aaccgtgtgt tgagatcttt ttattgcttg
    21841 attttatttt tattagctca gatacaaaat tcatcagtaa caaactaacc tgtctgtaac
    21901 tatcagatac agatctgtct ctctgcagaa aatgtttatt ggtatctatc agcgaaatca
    21961 gttcttcaat aaaattcaat tcataataaa taatgatcag cagattcttc atatccagtt
    22021 tcatatttga tctttcaatt tttatctttg aaaaatgcat cagtgatttt tcaaaatttc
    22081 ctttggcaga ttctatcatt gcctgagagt aatttgtgat ttcatcagaa agctcaggat
    22141 taagtctgtg tttatattca ctgagaaaat tatccagcca gtcaaactca tttaataatg
    22201 atgcattgcg cacaatattt gtaaaaaaat atggttctat aaatccatcc ggaatgagat
    22261 ctgccgcaag aagttttttg gtaatttcaa acatttgctt tcggtactca ggatatcctt
    22321 tctgtacttt tttgtggcag tactgcattg acactatctg tacattgtaa tcatcaatct
    22381 ttccgagttt tgctttaaga gtatccctgc tgattatgag ttttttaaaa taattttcat
    22441 cttcagtatt cagcagcatc agcatattaa aatacatact atataaggta acttctcttt
    22501 catcatccgc agacatatat tttaaaatct catcataaaa ctttagatca aattttttat
    22561 taagttgtct agaccattca ctgagacgga taaattgtaa aataacacct gtcagaaaaa
    22621 attctataag atcattcagt tcgttttgca aaccatctac aaaagaactt tcatccgagt
    22681 taagaaaata tccgttttgg atggaagtga acttcagctt ttgttcataa taataaatat
    22741 caagtgtgct gatatctaaa tttttaccgg taatgcggga tcttttttga aataaagcag
    22801 gcagctttct ttttctaagc atgtccagca ttttcacatt ataatcattt ttattttcct
    22861 taaaatcttc taatgtcaaa aagtctaata acagatttaa tgtaagtgaa attaatttcc
    22921 ttgtcattac gctgctgtat tttttatcag gatatacatt tttaaaaatg atttcttctt
    22981 tcagttcatt tgatttaaac tcaggaaaga atttttttat ataattaaaa aatctgatca
    23041 cttcacttcg gttattaaag taaggagacc ggataaattt cccaaactct ttaatttcat
    23101 ttttatctaa agtccttaga gattctatta actgggaatt ttccatggtt aaaattatgc
    23161 cacattaaaa aagtcaattt catataaaaa aatgaaaata tcaacatatt taagtaaatc
    23221 gttaaggttt gataatttgt ttgtgccaca atatggcaaa tctttctttg tttattcttt
    23281 atggaaaata gatttttgta tcgaagcagt cggagcttct ttaaaaattt cattaattaa
    23341 aaaaagttta aaatgaaaaa aattattttt tatctttgta ttgtaagttc atttgcagtt
    23401 ggtttcagag gtgggtgttt aatacatttt gtaaatttcg ctatacataa tccgttgggg
    23461 atagatgacg gattccttta tctaaacagt gatgcaggga tgaatgagaa ttattatatt
    23521 gcttcaggtg aaggcggtaa gatctggagg actactgatc ctgtaaatat tccatgggat
    23581 gagagagtca gtaatacaac aaatgatctg acatatgtaa atgacagcaa gagatcagac
    23641 acttctgtaa catatattgt tggcagatca ggaacaatag taagatccct tgatcaggga
    23701 atgacctgga ctttacatcc atccggagtt aataatgatt taaatggaat tgattttatt
    23761 ggttcaggta tagatcagat catagctgta ggagattcag gattagtatt aagatcatct
    23821 gacggaggag aaacatggtc gccggtaaat tccggagtta taaagaatct gaattctgtt
    23881 tactcattaa gttcatttat aactataatt gcgggagatg agggaacagt tctcaaatct
    23941 tcagacagcg gattgaactg ggtaaatcaa agtcttcctg atacaacttc tgatctgaat
    24001 aaaattagtt caatgggttc atggtttttt ggaccgatcc ttggaatggt cggtgaaaat
    24061 ggaaaattat attcatcgac aaattataat ttctggaatc ctgtagaaac aggtactact
    24121 gaaaatttat atgatctgca gtttaagaat tcaagttcag gatatgtaac aggtgagaat
    24181 ggaactatcc aatatactat tgatggtgga aatcagtggt tctcggaaat tttcttaagt
    24241 acaattacat cagaaaggat cagggcaagt attatgctaa atgatacaac agcagtcggc
    24301 gtagcaggaa gtgatataat tacgattcat gcaaatgaaa cactgctgcc tgtagaatta
    24361 gcttcattta attttaatgt tcagaataat aatgtcaatc tatactggtc aacattatca
    24421 gaaataaaca attcaggttt tgaagttgaa agaaaactct gtgatgagca acatcagcaa
    24481 aactgggtca ggatcagctt cgtggaaggt aatggttctg taaactcgac ttctgattat
    24541 gaatatactg ataaaggatt aaatgccgga aaatataatt acagattaaa gcagatagat
    24601 tttaatggta actttgaatt tcatgatctg tcaggagaag cagtgatcgg cattcctgat
    24661 aaatttgagt tgtcacagaa ttatccgaat ccgtttaatc cctcaacagt aataggatat
    24721 tcactgagtc aatcaggatt tgtaagtctg aaagtatatg atctttcggg aaaagaagtt
    24781 tatacacttg taaatgaaat tcaaactgca ggaaattacg aagtaacttt aaacggaagt
    24841 aatttatcca gcggagttta tttttataaa atgcaggcag gagaatttat ctcaaccaaa
    24901 aaaatgctgt taacgaaata aaattttaat caacccccga tgtgcagtct attatgacgg
    24961 tgtgactgat ctgtacatcg ggatttgaac atttactttc tcatcatatt taatttcgaa
    25021 ttcaacaaaa tcaaaaactc cttccctgct taattggaaa ggagttttta tttaattaaa
    25081 aattaaaaat taaaaattaa gatcaaaaca agctcaggta tttcaacatt caaaataaaa
    25141 cattgtacat tgaacattga aaattgtcac tactgtcttg ccattacatt tttcaaagct
    25201 acttcatgat cttcaatcag ttcaccgctt acatcaatta ttgctttata gatctttcct
    25261 gtaaaatcaa acggcggatc ataatcagga attgtcggtg aaccaggatc tgcaccaact
    25321 gcaatagatg cacccagtcc aattgaaaaa ggaattgtat aagggaaatc agattctccg
    25381 actaacttat catcaatata taactgagct tttccgggtg atccttttcc attagcaaaa
    25441 tcaggttttc ctgtaacttc aaattcaaat ctgaaattat gacgacctgc tggaacatct
    25501 tcggtagatt tcacataaaa atattctgca gtcacataat tatatccgta ttgaagtttg
    25561 ttatctttta taaataaagt atagcctccg tcacatcctc ccatactcat caatacacct
    25621 tcagctccat cctctggaat ttcagcatct acagtaatag tatgtgttct gtttaaaata
    25681 ttcacagatg catttgatgg aactggttga gtgcctggat aatatgtata ttttgttctc
    25741 ggcaaagcga tctgtggtct tggttcagca agtctttcta caccgcggct gtcaatcgga
    25801 agtacattat attttcctgc ttctatgtac cattgcgaga tcatttctat aagtttcgcc
    25861 ttattttctt tagcaagatt attcatctct gtcggatctt ttgtcagatc ataaagctcc
    25921 cacgcagttg catccagttc tgtaagttta tcagcagtga tcggcgcacc gaatcccatg
    25981 cctgcttcag taaatgattc tcccggccat ggacagacag ctctccatcc gttgtgataa
    26041 atagaacggt gtcccatcat ttcaaaatac tgaacttcat gactgctgtc tgcttttccg
    26101 tcattaaatg tatgcgcaaa actatttccc tgaatagggg actgagtaat tccgttgata
    26161 actgtcggcg gttctaaatc aagagcttcg agcacagtcg gcaccatatc aattgcatga
    26221 gcatattgtt ctcttatttc acctttggat tttatccctt ttggccagtg cacaataaag
    26281 ggatcactga ctccgcctct gtaagtttca cgtttccatc ttttgaaagg agtatttcca
    26341 gcgaatgtcc atccccacgg ataatggtta taatattttg gtccgccaat gtcgtcaatc
    26401 gctgct
//
LOCUS       DAORVQ010000024        25955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_674_length_25955_cov_5.531351, whole genome shotgun sequence.
ACCESSION   DAORVQ010000024 DAORVQ010000000
VERSION     DAORVQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 25955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25955
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_674_length_25955_cov_5.531351"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PLX80_03630"
     CDS             <1..1036
                     /locus_tag="PLX80_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY19919.1"
                     /translation="VFSFTKQLNGMLGLQYIHHTYELSNDKFKPYEFSIDYDFFTPRA
                     GVNYNFTNKLNAYINFSLAKREPRLKDIYDAEDPNSVPNFRIVDAANGIYEDALIQPE
                     KMQDLEIGFGYESEILKGGINFYNMNFENEIVNNGQLDNVGQPIVGNAGSSVHRGLEV
                     DFLLRPFFEKYYNGFTIGGNLTLSDNYFNEYIEILGADSVGNIIYGNDYSGNKIILTP
                     DVIGNLSLEFYPSAGTGAYFTMQYIGKQYLDNSENERKDPSLRDQAGYVDKFIDAYTV
                     FNAGISIDMMSFSKSKSIKNIFSKLQLDFKINNIFNSLYETSGYVSYGVPYWLPAATT
                     NYYAELKVGF"
     gene            complement(1350..2768)
                     /locus_tag="PLX80_03635"
     CDS             complement(1350..2768)
                     /locus_tag="PLX80_03635"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQY19920.1"
                     /translation="MIEKIKAVFNIKNLLLTLIFILSSSSINSQILYDVRGLSMGSTS
                     VSNSYDVDAFNQNPANIVFQRGGNNSKFYFNVLTTFQFLTNSDYLSLDFYNDYFGENT
                     DGSSTTLTESDKRNIINSASDQKSTLTSSGKLLSFLYNHGNIGTIGISLEDRIVSDFL
                     LTKDFWELGLYGNEPDRLYNLENNSLNVYWTRQLNISFAKLLKIKRNNLFETIAIGAA
                     VKPQFGIYYFETTKNNLTIQTNSQNTIQSTGESEFLYSGLTDDVTIKFPSGNAGFGLG
                     FDAGVNFILKKFSDQGKLSVGLSITDIGYIKWNTNNATYYNNGNFIITDVTNQEQIDS
                     LKDAVKSTKTPVSDFSISLPTTARLGVTYKIYTKKDKKLSGLEIGSISFDYIQGITDN
                     LGGTTKPRFGLGAEYNAGDIISTRAGLTFGGAENTLVSIGLGIEAGPVMIDMGTNNIM
                     SIFTPSGTTNLSAGINIKYKVN"
     gene            complement(2761..4284)
                     /locus_tag="PLX80_03640"
     CDS             complement(2761..4284)
                     /locus_tag="PLX80_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19921.1"
                     /translation="MFCSCEIKSPALPNYDIDLRIPFTIKNYNIFDIIDRSNNIGIDS
                     SNNNLLFIYGESNYRRIFGEDIKFDGIKKTFVTSASTLELDTIITINDSTFVTGMDFL
                     TGDLNFTFYNDLESNYSFDATIKNLFQKNSNDTVRINGTVNSGKNLTINLNLSDHYIK
                     NNKPDNQLYIRILFNSAVPVPVKFSYVLSEYSIKSMQGILKPLNTGVSFEEILDPFGS
                     DVPEGELSFSNITPDKNFFVVKKFTELYQVDFRNISLVGENKNGHRVRLKYLKYGEPG
                     DKIDTIFSLTLPQKDDSLAYPINENNSNILEFINNIPKKIFLERTDFLNFDYQEGAVN
                     YTDSLSLKLILQVPLNVSITKPVIFRDTADVGIDNEEQRNNLDKAKKLIFTLTSVNGI
                     PLKNVAKVLVLDSNFNPLLPITLMISNNQDSSVIFNAGSVGPDGYSSLPTVTSYSAEL
                     DSTDISKIKRMGKIIFENQLFTDPNYIPDPFNNVKIRSGDKISIAGFGTLSYRLDFD"
     gene            4675..5754
                     /gene="recA"
                     /locus_tag="PLX80_03645"
     CDS             4675..5754
                     /gene="recA"
                     /locus_tag="PLX80_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQY19922.1"
                     /translation="MAEDKESKMKSLDLALEQIQKEYGKGAIMKLGDKPILKLASIST
                     GSISLDAALGIGGVPRGRILEIYGQESSGKTTICLHIIAEAQKNGGLAAFIDTEHALD
                     TGYAQKLGVDVNNLLISQPEYGEQALEICETLVRSNSLDVIVVDSVAALTPRAEIEGE
                     MGDAVMGMQARLMSQALRKLTAAVSKSNVVLIFTNQMREKIGIMFGDPNTTTGGKALK
                     FYASVRMEIKRIAAIKDGTDVIGNRTRVKVVKNKVAPPFKEVEFDIIYNEGISKIGDL
                     IDMAVNKEIIKKSGAWFAYDDNRMQGRDGVKKFMQEDRKTLMKLYAEVKKSLGITDDA
                     EEPTNGKAPLAETVTADEDSSSKKK"
     gene            5812..6435
                     /locus_tag="PLX80_03650"
     CDS             5812..6435
                     /locus_tag="PLX80_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HQY19923.1"
                     /translation="MKISGIEKQKKDKQRYNILIDGEFYTGLYEDTIFKFGLRIGDEI
                     SEKDIEDIKDYDEFNYAKTCAYRSLAYRQRSKKELITKLKQKKISLPVIMKVIDLLEE
                     QKYLNDEDFAENFVKEKIKNKPSGKRTLIFKLQEKGISKEISEDVTAKNYSAEDEYAA
                     AAKLLTKYIKKGRFKDDYDKKSKCFRHLVSKGFNFDTANRLLSETEL"
     gene            6726..7940
                     /locus_tag="PLX80_03655"
     CDS             6726..7940
                     /locus_tag="PLX80_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY19924.1"
                     /translation="MENMIYSEFSKYEEELKESFSQKYSLPDIEIDLIVKDLRTIVMQ
                     MIKLMRDGKSVHEKFFSDIILNSVDAIIGFDNNLKIFLWNKGAELIFGYSKNEIMGKE
                     ISELVPVYLQDKGEIEFLVKEVDQKGFITNYESERLTKKGEIINVSITRFTIFNENKE
                     KIGSVGTVRDITTIKNLEKELREKEKLGLIGEVVSSIAHSLSNPLNIISGNADYLLMD
                     KSENDEGYEELKCILDETIRITKSIRHLLNFSRPLNISREKNNINDIIEKAVSGNKFL
                     IKDKKISFRKILNPNIPEINIDKSEIEETICNILTNAVQAIKNSGEITIKTGSCNDHI
                     NIEISDNGEGIPKVNLEKIFTPFYSSKEYGKGTGLGLSIAKRIIVEHGGEISATSKPG
                     KGTKFFIKLPVK"
     gene            8193..10118
                     /locus_tag="PLX80_03660"
     CDS             8193..10118
                     /locus_tag="PLX80_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19925.1"
                     /translation="MKKLYILIFVILFSDLSYSQTGNSPYPVIFIHGLNSDDMTWNTT
                     LNQLSNTWNLSSNNTFHAVLNARGGDTTEFSQDVIFPLKDVNGNIVNSISNSDIFSIN
                     FGNFWNRDITDPRIILYNNSTPGSNQNASNQSAIIKQGYALKIMIDSVLNVTGAEKVI
                     LAAHSMGGLAIREYLQRRENGFHKWWIDPNDTLNGHKVAKVITIGSPHQGTNVTLQVG
                     GIDFNSEAIRDMRSSFSGSSVAGYIFGNLESAVPSTYYNKDQNCNGTENDTIIGINSG
                     TIDNVNMSLPDNIQYTWIMSNYLGLGTDLAVPYSSQAIYNGSIFAPPVVSDTIRTNKN
                     HIQETSDSRSLIRALDEPDTKDFAYDISFNKSYAGFITLQSKVITADSDYYKLKTLTG
                     GKITVTMNSVNAGVTNISLLNQDGSIIVSKNISNSPDSIIYFSPPGEYFLKIIGNSNQ
                     NLNLNSYSFNTGIVPAAMLNLTLGIEGFWDGVSQIEDTVRVYLTNSFSPFEIKDSAFV
                     VLNSSGNGLVNFIHTPIGYNYVKIIHRNALELWSKVPVSFTGGIITDFDFTSDQEQAF
                     GNNQILKSGRWCIYSGDTDHDGIIDVSDQSEIDNDVSNITTGYVNSDLTGDSFVDLSD
                     ASIADNNGFNFVSVIKP"
     gene            complement(10213..11196)
                     /locus_tag="PLX80_03665"
     CDS             complement(10213..11196)
                     /locus_tag="PLX80_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19926.1"
                     /translation="MKNFHKISLLIFFCFALIIFNNSNSYPDPINTSNSDFNVVPPAY
                     SNLPGTATFLGPLANSPRSYQLLIHANQITALINSRINSFTFRIPVSATADWPATPVT
                     FTNFDVYLSGCVDPVNRSLTFVNNVVGPQTLVRSGVLIIDSASFKSGATPNEFGMEIE
                     LDSGWIYESGNLLIELRHTGFTGTSRSVDAIGTAIPGYGTDFSACWQSSYTPTTGLQG
                     NFAVTRINSDMLVGISNNNEIPDSYKLEQNYPNPFNPKTVIRYSIANTSEVKIKVYDI
                     LGNEVKTLVNSKQNAGSYETEFDGSELSSGVYIAKLTSGNYSTQIKMILNK"
     gene            12245..12835
                     /locus_tag="PLX80_03670"
     CDS             12245..12835
                     /locus_tag="PLX80_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY19927.1"
                     /translation="MLNKELYARDISGDVVLLKKISTGDTESLRKLYNQYSDYLFKIV
                     FYILKNESDAEDTLQEVFMQIWEHSEKYDEKLGSPISWIVRVTRNKSIDKLRSKGYRE
                     RENQFDIDRFYNLSSDEPSENPDSVMTINSTSEQIKSAIDTLSDIQKELLEFTYFRGF
                     SQSELAEHFELPLGTVKTCIRTAMIKLRDKLKHLIN"
     gene            12838..13428
                     /locus_tag="PLX80_03675"
     CDS             12838..13428
                     /locus_tag="PLX80_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY19928.1"
                     /translation="MENNNNIIDDHIIFRSLGMEFGEIDSDSGVNENNDQIQKIREYN
                     EIASLIPSVLNDVNFSNTFTSKKKESIFENVLSEIDRKSKLEKKHENFSFVFDNNSEW
                     INYPIKGIKFKPLSLNKEKNYLMLLLKAEAGCQYPSHHHSGAEECYVIEGDLCAEGKV
                     LGPGDFHHAEGDSDHKSLFTKNGCTLLIVADPEDYT"
     gene            complement(13621..14241)
                     /locus_tag="PLX80_03680"
     CDS             complement(13621..14241)
                     /locus_tag="PLX80_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQY19929.1"
                     /translation="MFSLPGIVFSHPIHDLANMSTIDTGWVYVQLGFTHIIPYGLDHI
                     LFILGLFLLSPSLKPLLIQISVFTIAHSITLSLAVFNIVNIPGFIVEPIIALSIVFIA
                     IENIYSRKLNSFRITVIFLFGLIHGLGFADVLKDLGLPKEQFLNALISFNIGVEFGQI
                     AVVLAAFITVGIWFRAESWYRKRILIPGSLIIALIAFYWAAERIFQ"
     gene            complement(14318..15604)
                     /locus_tag="PLX80_03685"
     CDS             complement(14318..15604)
                     /locus_tag="PLX80_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY19930.1"
                     /translation="MKKIFIISSVIIVLSLITWKVFDKNTEQTVSPQGIYDNDINYIS
                     NTNSSADRYISILKEQLTEAPENTNILNKLGAAYIQKARETNDPEYYASAENVLNRSL
                     DLQQENFLAYAELGSVKLSRHHFREALELSEKSLTQNPYSAYSYGVLVDAQVELGMYD
                     EAIISLQKMVDLRPDLSSYSRISYVRELFGDTEGSIEAMRSAVNAGSPVAENTVWCKY
                     QLANLMYNAGNTDTASELFQEIIFAYPEYSYAYAGMAKIKMHYNKYSEAIELYNKAVQ
                     KNSVPEFLISLGDAYTLNGEIENAEEAYNKVKFLITFYKQNGVETDLELALFNADHGI
                     RVDESFEISEISLQEGSRCIKTFHAAAWTAFKSGNINAAENYISQALRLGTKDPLLYY
                     HAGKIFEKTGKHSEANEYLKFALNINPFYESLYVQN"
     gene            complement(15659..17422)
                     /locus_tag="PLX80_03690"
     CDS             complement(15659..17422)
                     /locus_tag="PLX80_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HQY19931.1"
                     /translation="MLKKKFLYSLFGVLAVCTALFSLSQWTDASSHREAPIISTDPVA
                     DNTDVYAFRPADDPGMVCLIACYNPLEEPAGGPNFHKFGTDVLYEIKIDNVGDRIDHI
                     SYQFEFTDIVGNGNTFLYNTGEVSTLGDPDLNVKQTYTVKRVVNGVTEWTSSPLPVTP
                     NYVGGNSHNDPNYAGPSSMPNYAGLMSEGVRDLPSSGGKVFAGPTDDPFFVDLGAAFD
                     LLKIRPGAPGNGGGGKDALAGYNVHSISIKVPIAQVTRNGSPNPNQNDSNAIVGVWAT
                     ASRKATTVINSNGTRTGSGNWVGVSRLGMPLVNEVVLPLSQKDKWNASKPVDDVQFLN
                     YVTSPELAGIINSLYPVTDDIPTTNRTDLVAVFLTGVPGLTQRPQDTRTPSEQLRINL
                     GIPPVAFENENRLGVIDGDVAGFPNGRRLKDDVIDIALRVVGGVLLGPPFSTGINAQL
                     GDAVQRNDKSFASAFPYLAIPSEGYENSHGTIVSIAANSNQNINPEKFGLIQNYPNPF
                     NPSTNIKYNLLKADNVDIRIYDILGKEVRTLVSEKLAPGERVVVWDGMNNNGVAVASG
                     TYFVKLVTSSGVDSKKMLLLK"
     gene            18101..18895
                     /locus_tag="PLX80_03695"
     CDS             18101..18895
                     /locus_tag="PLX80_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19932.1"
                     /translation="MKKAFLISIVTFVIILTNSILVYTYEDGITGRTKKTSANGCSCH
                     SMNTNITGVFSGPDTVSAGETVVYSFTINRSGSGAGGGADISTRLGALGVGPGASYLR
                     IQNGELTHQSPLGLSGGSFTGQFNYTAPSSPGTDTLWISPAVGYQNGWNWGTEKRIVV
                     KNLVGINNLNNPVYYKLYQNFPNPFNPSTNIRFEIPKSEFVSLKIFDITGKEISSLVN
                     ENLQAGVYNIRFDLNNSIYHQLTSGIYYYILKTENFTETKKMMLVK"
     gene            19051..19518
                     /locus_tag="PLX80_03700"
     CDS             19051..19518
                     /locus_tag="PLX80_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19933.1"
                     /translation="MDKISELLSHKEFTSEWEKSNKDINAAKLLTLRQVEKFEDIVSD
                     DVSWEKLLEIFNSGRETDAWLAMDWPEGFDELMLCVPLCKLVDFKCSECTVGMRQRNN
                     SCANDFSLFGYIAELLKAADREGLKDHLGSVKKILLLENFLWNIKEKRTEEII"
     gene            complement(19908..20555)
                     /locus_tag="PLX80_03705"
     CDS             complement(19908..20555)
                     /locus_tag="PLX80_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19934.1"
                     /translation="MIKIFILYIVFSLIFISCGEEKKPEILVKKDSVEMIVKEDTTLI
                     VYKEHETKTGKKFIIIESKPTESVSNYYVTGSGFNNFKDTLSFPLKNPMHNSLIADID
                     GDGFEELYIITKSTGSESFLNIFGAASNGDTALAEIKAFDTSTEEYQKDENLAGYLGN
                     DSIYFSGKNIIREFPVYKSSDLAEDNTKGKKRITYKLKKGDEFYELEISEVKDIK"
     gene            20676..22040
                     /locus_tag="PLX80_03710"
     CDS             20676..22040
                     /locus_tag="PLX80_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY19935.1"
                     /translation="MILYIFELMENEFKILVVDDEPNSTKLMRKILSAGGYKAEEENN
                     SVKAKEKISTGDYDLVISDLQMPEVSGLDLLRSKPSDTNFIMVTGFGSVDSAVESMKS
                     GAFDYLSKPFNIDEFKIKVKKALDNIKLNQRLKDLKNEIDKNHNFGNMIGRSRKMKSV
                     FEMIKNVSETNVNVLVEGQSGTGKELVSRAIHNNSPRKDNPFIAINCSAIPENLLESE
                     LFGHTKGAFTGATENQKGVFEQANGGTLLLDEIAEMPFHLQSKLLRVIENWEIKPIGS
                     DKIKKVDVRLISATNQNINELILEKKFREDLYYRISTVTIVMPALTERKDDIPLLTDH
                     ILKKLSVRYDKNYSIDADALELLMNHNWKGNIRELENMLERTALSSADNVIKKSDFRI
                     SDSGGGNMNDSISNITGGLKELEKIYISKILDENKWNKLKVSQILGIDRKTLYKKIRE
                     YNLE"
     gene            22309..22668
                     /locus_tag="PLX80_03715"
     CDS             22309..22668
                     /locus_tag="PLX80_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19936.1"
                     /translation="MKRIIHIICIDDSICQMIQKTLRSKGYEVSISDGNKLSVKNIIE
                     LSHDIECFVIDSGIDEGLFSNLKLKFCNSSYIFLPSLNKDSKKINGDYSNISEPLKLS
                     ELFQTLDSVFKEKEIEN"
     gene            22658..24592
                     /locus_tag="PLX80_03720"
     CDS             22658..24592
                     /locus_tag="PLX80_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQY19937.1"
                     /translation="MKINIKYIAAFLISLCLIIFISISAVEYTTSKSKDIDDCLACHE
                     DSELQMESGTKQISVFVNPELYKKSVHGSAECSDCHLNYNPDEIPHSKNSKAVECSTC
                     HDDLKGIEHSVHAKTECVDCHGKHDIMPVKETQAESSANCLSCHKKKSITQFSSSIHS
                     KKGVKCNDCHGDGHQVKKISRNQITDLCGKCHNDSKMNFKGSIHHSAIKDGNKNKNTP
                     VCTDCHGSHKIVTSKFKIESEACMKCHLDEKLFPGEETGSAKFVAQYKTSVHASIGKG
                     GMESAGCTDCHGDHMIQSPDDPKSSTKRAQLVETCGKCHADVVANFKKSKHGKELINN
                     NKDAPTCSDCHGEHNIQATLSSDEFSKLNIANKCISCHEKSKLAEKTKEGNDEMVIDF
                     KNSVHYKALLKGDMSSATCSDCHGAHEMDKPDDPHSRVNRNNIVSTCGQSNCHSSELA
                     EYSGSVHDISQSEKENPNVPTCNTCHGNHIILKADEKENKLSYSKGIVQTCSDCHDSR
                     VINEKYDIKTKQVETFKESIHGIYIQFGDEKAANCASCHGTHNIRREDDPQSSIHQSN
                     ISKTCGQTDCHPNASVNFVKGKIHLDPKSKDSGFIYYVSTFFKYFTFTILFSLFLYIV
                     LDLRSKIRERKRPKSENNDE"
     gene            24582..25565
                     /locus_tag="PLX80_03725"
     CDS             24582..25565
                     /locus_tag="PLX80_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQY19938.1"
                     /translation="MMNSSGSNIETNSTEFQRFGLNIRLQHIILMVCTILLIISGLPL
                     KFYTTDWAKSVINLLGGTDGARKVHHFAALGLIVIGIYHLLFSIINRSGRRDFMLLIP
                     GKKDMNDFFHQLQYFFGKKKYKPKFGRFSFIEKFDYWAVYWGFIIMIGSGIIMWMFQG
                     KFYPMFFGEVDISFTSGIVDNYVHAISREAHSDEALLATLAILIWHFYNVHFNPDKFP
                     GSKVWFSGKMTRKEMEEEHPLELEEIENRLNKKEMNIEKDGTVVSETEQTEVDTKVSD
                     VIKPDTKPDNVSNDISDQKNNITDSSDGNAKSNDKTSGTKDPDNEKSNKVK"
     gene            25634..>25955
                     /locus_tag="PLX80_03730"
     CDS             25634..>25955
                     /locus_tag="PLX80_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQY19939.1"
                     /translation="MKKISFILLLTAVFAVIAILINIEITKSQPQSQAKYVGVTTCLG
                     ACHKSEKQGNQLEIWKNSKHSQAFKSLQTPFADSIANAKGHSTAAAETPECVVCHVLG
                     REMMP"
BASE COUNT         9106 a         4015 c         4639 g         8195 t
ORIGIN      
        1 agtttttagt tttacaaaac aactcaatgg aatgttagga cttcagtata tacatcacac
       61 atatgaattg agtaatgaca aatttaagcc ttatgagttt tcaatagatt atgatttttt
      121 cactccgaga gccggagtga attataattt tacaaataag ttaaatgcat atataaattt
      181 ttccctggct aagagagagc caagattaaa agatatttat gatgcggaag atccgaacag
      241 cgtgccaaat ttcagaatag tggatgctgc aaacggaata tatgaagatg cactgattca
      301 gccagaaaag atgcaggatc tggagatcgg ctttggttat gagtcagaaa tattgaaagg
      361 aggaattaat ttttataata tgaatttcga aaatgagatt gtaaataatg gacagcttga
      421 taacgtggga cagccgattg ttggaaatgc aggcagttct gtacatagag gacttgaagt
      481 agatttttta ctcagaccat tttttgaaaa atactataat ggatttacta taggaggaaa
      541 tctgacattg tcggataatt attttaatga gtatattgaa atacttggag cggattcagt
      601 tggaaatata atttatggaa acgattattc aggaaataaa ataattctga cacctgatgt
      661 aattggaaat ctttctctcg aattttatcc gtcggcaggt actggtgcat atttcacaat
      721 gcagtatata ggcaaacagt atctggataa ttcggaaaat gaaagaaaag acccgtcttt
      781 gcgggatcaa gccggttatg tagataagtt tatagatgct tatactgtat tcaatgcagg
      841 aatttcaatt gatatgatgt cattttcaaa aagtaaaagt attaagaata tattttcaaa
      901 acttcaactg gattttaaaa taaataatat atttaattca ctgtatgaga catcgggata
      961 tgtaagctac ggagtaccgt actggttacc ggctgctact acaaattatt atgcggagtt
     1021 aaaggttgga ttttgataat tgggaaagtc tggaaagcat atataggaaa atattatgag
     1081 aataaatttc gaagtatatt tttgtttggg aaataaatcc gacagaggat aatcgatcag
     1141 atagataaag tattagtcga aacctgacag gttttcaaaa cttcagtttc acaataaatc
     1201 ttcattttta aacctgtcag gttttgcacc aatatacaaa acctgacagg tttccaattt
     1261 tccggtttca tctttaatct tcacctgcaa aactgtcagg tttcatttcc acactccaaa
     1321 cccaccaact aattccgcat catccggctt cagttcactt tatattttat atttataccc
     1381 gcagaaagat tcgtcgtacc cgatggagta aatatgctca ttatattatt cgtccccata
     1441 tcgatcatca caggtccggc ttctattcca agaccgatac ttactaatgt attctcagct
     1501 cctccaaaag tcagtcctgc tcttgtagaa attatatctc ctgcattata ctctgctcca
     1561 agtccaaatc taggtttagt tgtacctcca agattatctg taatcccctg aatataatca
     1621 aatgatatac tgccaatttc aagtcctgaa agttttttat cttttttagt ataaatttta
     1681 tatgtcacgc caagtcttgc tgtagttggc agactgatac tgaaatcaga tacaggtgtt
     1741 ttcgttgatt ttacagcatc cttaagagaa tcgatctgtt cctgatttgt tacatcggta
     1801 ataataaaat ttccattatt ataatatgtt gcattgtttg tattccattt tatatatcca
     1861 atatcagtta tactcaatcc aacactaagt ttcccctgat ccgaaaactt ttttaaaata
     1921 aaattaactc ctgcatcaaa tcctaatccg aatcccgcat ttcctgatgg aaattttata
     1981 gtgacatcat ctgtaagccc tgaatataga aattcagatt cacctgttga ttgtatagta
     2041 ttctgtgaat tagtttgtat tgtaagatta ttttttgtgg tctcaaaata atatattccg
     2101 aactgaggtt ttacagccgc accgattgct atggtttcga ataaattatt tcttttgatc
     2161 tttaataatt tagcaaaaga aatgtttagc tgccttgtcc agtaaacgtt gagagaatta
     2221 ttctcaagat tatataatct gtcaggttca tttccatata accccaattc ccaaaaatct
     2281 tttgtaagca aaaaatccga aactatcctg tcttcaagcg agatacctat agtaccaatg
     2341 tttccgtgat tatacagaaa ggaaagtaat tttcctgatg atgtcaaagt ggatttttga
     2401 tcacttgctg aatttataat atttcgttta tcagattcgg tcagagtagt tgagcttccg
     2461 tctgtgtttt ctccgaagta atcattataa aaatccaatg aaagataatc actgttggtt
     2521 agaaattgaa aagttgttaa tacattaaaa taaaatttag aattattacc acctctttgg
     2581 aaaactatat ttgcaggatt ttgattaaag gcatctacat cgtatgaatt tgacactgac
     2641 gttgaaccca ttgacagacc tctgacatca tacaatatct gtgaattaat actgcttgat
     2701 gaaagaatga aaattaaagt gagaagtaaa tttttgatat tgaaaactgc ttttattttt
     2761 tcaatcaaaa tctaatctgt atgaaagtgt tccaaatccg gctatactaa ttttatcacc
     2821 cgaacgtatt tttacattgt taaacggatc aggtatataa tttgggtctg taaacaactg
     2881 attctcaaat attatcttac ccatacgttt gatctttgat atatcagtac tgtctaactc
     2941 agcactgtat gaagtaactg ttggtagaga agaatatcca tccggaccta ctgatcctgc
     3001 attaaatata actgatgaat cctgattatt acttatcatt aatgttatcg gaagcagtgg
     3061 attaaaattt gaatccagca ccaaaacttt cgccacattt tttaatggaa ttccatttac
     3121 agaagttaaa gtgaaaatta attttttcgc cttatcaaga ttatttcttt gttcctcatt
     3181 atctataccc acatctgccg tatctctgaa tataaccggc ttcgtaatac tgacatttaa
     3241 aggtacctgt aagatcagct ttaatgaaag actgtctgta taattgacag ctccttcctg
     3301 ataatcaaaa tttaaaaaat cagttctctc gagaaagatt tttttaggga tgttgttaat
     3361 aaattcaagt atgttgctgt tattttcatt tatcggatat gcaagtgaat catctttttg
     3421 aggcaatgta agtgaaaaga ttgtatcaat tttatcaccc ggttcaccat acttcaaata
     3481 ttttaatctg actctgtgac catttttatt ttcacctact aaagaaatat tcctgaaatc
     3541 gacctgatat agttcagtaa atttttttac gacaaagaaa tttttatccg gagtaatatt
     3601 tgaaaaagaa agttctcctt ccggtacatc tgaaccaaat ggatcaagta tttcttcaaa
     3661 actaactcct gtatttagcg gtttcaaaat cccttgcatg ctcttaattg aatactccga
     3721 caatacataa ctgaatttta caggcactgg aactgcggag ttaaataata ttcttatata
     3781 aagctgatta tcaggtttat tattttttat ataatgatca gataaattta aatttattgt
     3841 taaattcttt ccggagttca cagttccgtt tattctgaca gtatcattac tgtttttttg
     3901 gaataaattt tttatagttg catcaaatga ataattgctt tcaaggtcat tataaaatgt
     3961 aaagtttaaa tctcctgtca gaaaatccat accggtaaca aaggttgaat catttattgt
     4021 aattatagta tcaagctcta atgtacttgc agaagtaacg aatgtctttt ttattccgtc
     4081 gaatttgata tcctctccaa agattcttct gtaatttgat tctccgtaaa taaacaaaag
     4141 attattgttg gaagaatcta ttcctatatt attgcttctg tcaataatgt caaaaatgtt
     4201 ataatttttt atagtaaaag gaatcctcag gtcaatgtcg taatttggga gtgcaggaga
     4261 tttgatctca caactgcaga acaaaattga aataataaaa aaagaaataa attttgcggt
     4321 aaaaaatctg atatgcataa agtagattgc aatattgatt ctatagcaat ctttgaaagt
     4381 taataatttt ttttattaat ttagttaaat atatttgaaa ttaaactgaa ttatttaaat
     4441 tctctatata gctctgaatt tcaatttatg atcttagaaa agaaaaacca ttttcacaaa
     4501 catataaaat tgcaatttga ctttccaatg taataacgat aatttacacc agtaagtctt
     4561 gatgaaactc ataatatcag agactttgaa aattgaagat aaaaatcaat ataaacttta
     4621 aatgaaattg aatatattaa cacacctaat aataaattaa accaatcaaa taaaatggca
     4681 gaagataaag aatcaaaaat gaaatctctc gatttagctc tcgagcagat acagaaggaa
     4741 tatggcaaag gtgctattat gaaactcggt gataagccga ttttaaagct tgcttcgatc
     4801 tccactggtt cgatctcttt agatgcagca ctgggaattg gcggcgttcc aagaggcagg
     4861 atcctcgaaa tttacggaca ggaatcatcc ggtaagacga ccatctgtct tcacataatt
     4921 gctgaagctc agaaaaatgg cggtcttgca gcgttcattg atacagaaca tgcacttgat
     4981 acaggatatg ctcagaagct gggtgtcgat gtcaataatc tgctgatttc acaacccgaa
     5041 tacggtgagc aggctcttga gatctgcgaa actctggtaa gaagtaattc tcttgatgtt
     5101 atagttgtcg attccgttgc agctcttaca ccaagagctg agatcgaagg tgaaatgggt
     5161 gacgctgtaa tgggaatgca ggctaggctt atgtctcagg cattgagaaa attaactgct
     5221 gcagtttcaa aatccaatgt tgttcttatt tttacaaatc agatgagaga aaagatcggt
     5281 attatgtttg gtgatccgaa tacgacaaca ggcggtaaag ctcttaaatt ttatgcatct
     5341 gtgagaatgg agatcaaaag gatcgctgct attaaagacg gtactgatgt gatcggaaac
     5401 agaacaagag tcaaagtggt aaaaaataaa gtcgctccgc cttttaaaga agtggaattt
     5461 gatattattt ataatgaagg aatttcaaag atcggtgatc taatcgacat ggcagtgaat
     5521 aaagaaataa ttaagaaatc cggagcgtgg tttgcttatg atgataacag aatgcagggc
     5581 agagacggtg tgaaaaaatt catgcaggaa gaccgaaaga cacttatgaa attatatgct
     5641 gaagtaaaaa aatctctcgg tattacagat gatgcagaag aacctacaaa tggtaaagct
     5701 cctctggctg agacggttac tgctgatgaa gattcatctt caaaaaagaa ataattaatt
     5761 tgtgtgaatt ataatatact gctgatctga aaatggtcag cagttttttt tatgaaaata
     5821 tcaggaatag aaaaacagaa aaaagacaaa caaagatata atattctgat agatggtgag
     5881 ttttataccg ggttatatga ggatactata tttaaatttg gtttgaggat cggagatgag
     5941 atcagtgaaa aggatattga agatataaaa gactatgatg aatttaatta tgcaaagaca
     6001 tgtgcataca gatcacttgc ttacagacag cgaagtaaaa aggaattgat cacaaaactc
     6061 aaacagaaaa aaatttcttt acctgttatt atgaaagtaa tagatcttct cgaagaacag
     6121 aaatatttga acgatgagga ctttgctgaa aattttgtga aagaaaaaat taaaaataaa
     6181 ccatcaggga aaagaactct gatattcaaa cttcaagaaa aaggcattag caaagagatc
     6241 tctgaagacg taactgctaa gaattattca gcggaagatg aatatgctgc tgcagcaaaa
     6301 ctactgacta aatatataaa aaagggaagg tttaaagatg attatgataa aaaatctaag
     6361 tgcttcagac atttagtatc aaaaggtttt aatttcgata cagccaaccg cctgctgtct
     6421 gaaacagagc tctgaattta ttgaaatact ttattctatc cattgaatac tacctgacat
     6481 tgcatacttt aattgtttaa gttatagtac atcctattct taaaacctaa tcacttactt
     6541 cttttaggaa cttcaaaatt tccgaaaacc ggaaatatca ggacttaaaa tttccaatac
     6601 aattatgata attatcatat tcagtattga ccactgtcat taagtaattg tgtcgatttt
     6661 gctttattat taataaaatg atgtatcatt tttattaatc tttaaaataa gtacagtaca
     6721 agcatatgga aaatatgatt tattcagagt tttcgaaata tgaagaagaa cttaaagaaa
     6781 gtttcagcca aaaatacagt ttacctgata ttgagattga ccttattgta aaagatctgc
     6841 ggactatagt aatgcagatg atcaagttaa tgcgagacgg taaatcggtt catgaaaaat
     6901 tcttttcgga tataatactg aattctgtag atgcaattat tggttttgac aataatttga
     6961 aaattttcct ttggaacaaa ggcgcggagt taatctttgg gtattcaaaa aatgaaatta
     7021 tgggaaaaga aatttctgaa ttggttccag tttatttaca ggataaagga gagattgagt
     7081 tcctcgtaaa agaagtggat caaaaaggat ttattacaaa ttatgaatca gaaagattaa
     7141 ccaagaaagg tgagattatt aatgtcagca taaccaggtt tacaattttt aatgaaaata
     7201 aggaaaagat cggatctgta ggaacggtca gagacattac aacaattaaa aatcttgaaa
     7261 aggaactgag agaaaaggaa aaacttggac ttattggaga agtcgtttca agtattgcac
     7321 acagtctgtc aaatccatta aacataattt caggtaatgc agattacctc ttaatggata
     7381 aaagtgaaaa tgatgaaggg tatgaagaac tgaaatgtat tcttgatgaa actatcagga
     7441 tcacaaaatc catcaggcat ctgttaaatt tttccagacc attaaatata tcccgtgaga
     7501 aaaacaacat taatgatata attgaaaaag ctgtttctgg caataagttt ttaattaaag
     7561 acaaaaagat ctccttcaga aaaatcttaa atcccaatat cccggagatc aacattgaca
     7621 aatccgagat agaagagact atttgtaata ttttaactaa tgctgtacag gcaataaaaa
     7681 attcaggcga gatcacaatt aaaacaggtt catgtaacga tcatatcaat atcgaaatct
     7741 ctgacaatgg tgaagggatc ccaaaggtaa acctcgagaa aatttttact ccattctact
     7801 catccaaaga atacggcaaa ggaacaggtc ttggtctttc aattgccaaa cgaattattg
     7861 ttgaacacgg aggagagatc tctgcaactt caaaacccgg taaaggtaca aagtttttta
     7921 taaaactacc tgttaaataa attcggatta cttaatctta ctgctaattc ttataattta
     7981 ttttgaaaat tatttagcct gcttaaatta atttttaagt aaaaactcca ggcaatttat
     8041 cacctctgac gactttgatt ggtcgttcaa aaaaagcgtt ctaaacagtg ttgatttaaa
     8101 tatcaataat tttgttaaat aaacattgat gattctgatt taaattttta ttttaaattt
     8161 actttctaac aaactgacta aatattttta aaatgaaaaa attatatatt ctgatatttg
     8221 taattttatt ttcagattta tcttactcac aaacaggaaa ttctccttac ccggtaattt
     8281 ttattcacgg tctgaattca gatgacatga cctggaatac aactttaaat caactcagca
     8341 acacttggaa tctcagtagt aataatactt tccatgctgt gctgaatgcc agaggaggtg
     8401 acactactga atttagtcag gatgttatat tccctctcaa agatgtcaac ggtaatatag
     8461 taaactcaat ttcaaactcg gatatatttt ccatcaattt cggaaatttc tggaacagag
     8521 atattacaga ccccagaatt attttatata ataattcaac acccggcagt aatcagaatg
     8581 caagtaatca gtcggcaatt atcaaacagg gctatgcttt gaaaattatg atagactcag
     8641 ttcttaatgt tacaggtgct gagaaagtca tactggcagc gcattctatg ggcggacttg
     8701 cgatcaggga atatctgcag agaagagaaa atggttttca taaatggtgg atcgatccga
     8761 atgatacact aaacggacat aaagtcgcaa aagtaattac tatcggctct ccgcatcagg
     8821 gaactaatgt aactctgcag gtcggtggaa ttgattttaa cagcgaagca atcagagata
     8881 tgagaagttc attttcagga agttcagtcg ccgggtatat cttcgggaat ctggaatctg
     8941 cagtgccttc aacatattat aataaagatc agaattgtaa cggaaccgaa aatgatacaa
     9001 taataggaat taattcaggt acaatagata atgtaaatat gtcattaccc gataatattc
     9061 agtacacctg gatcatgtca aactatctgg gacttggcac tgacctcgct gttccataca
     9121 gcagtcaggc aatttataac ggaagtattt ttgctccgcc agttgtttca gatactatca
     9181 ggacaaataa aaaccatatt caggaaacca gtgacagcag atctttgatc agagcattgg
     9241 atgaaccgga cacaaaagat tttgcatatg acatttcatt caataaaagt tatgccggct
     9301 ttattactct gcagagtaaa gttattacag cagattcaga ttattataaa ctaaaaacat
     9361 taaccggcgg aaaaattact gtaacaatga attcagttaa tgcaggagtt acaaatattt
     9421 cattgctcaa tcaggatggc agtataatag tttctaaaaa tatttcaaat tctcctgaca
     9481 gtattattta tttttcacca cctggagaat attttcttaa aattataggt aacagtaatc
     9541 aaaacctaaa tttgaattca tatagtttta atacaggtat tgtgccggct gccatgttga
     9601 atctgacttt gggtatagaa ggattttggg atggagtttc acaaattgaa gatactgtga
     9661 gagtttatct cacaaacagt ttctcgcctt ttgaaataaa ggactctgca tttgttgttc
     9721 taaattcttc aggaaacggt ttggttaatt ttattcatac accaatcgga tataattatg
     9781 taaagatcat tcacagaaat gctcttgaat tatggagcaa agtgccggtg tcatttacag
     9841 gcggcatcat tacggatttt gattttactt cagatcagga acaggctttc ggaaataatc
     9901 agatcctgaa atcagggagg tggtgcatct acagtggtga tacagatcat gacggaatta
     9961 tagatgtgag tgatcagtct gaaattgata atgatgtctc aaatattact acaggatatg
    10021 tgaattctga ccttacaggt gattcctttg tagatctgtc tgatgcttcg attgctgata
    10081 ataacgggtt taattttgta agtgtaataa aaccctgatg attaatttta aattaaaaat
    10141 gttcaacgtt caatattttt aaaataaaca ttgaacattg aacattgaac ataaacatta
    10201 aattttgaac aattatttat tcagtatcat ttttatctgc gtagaataat ttcctgaagt
    10261 aagctttgca atatagactc cacttgaaag ttcgctgccg tcaaattctg tttcataact
    10321 acccgcattt tgttttgaat ttactaaagt ctttacttca tttccaagaa tgtcatatac
    10381 tttgatcttt acttcgcttg tgttagcaat actatatctg atcacagttt taggattgaa
    10441 cggatttgga taattttgct cgagtttata actatcagga atttcattat tatttgaaat
    10501 gccaacaagc atatctgaat taattcttgt gacagcaaaa tttccctgca gacctgtcgt
    10561 cggagtataa ctgctttgcc agcaggcact gaagtcagtt ccgtaccctg gtatagcagt
    10621 tccgattgca tcgacacttc gtgaagtacc tgtaaatccg gtatgtcgaa gttcaattag
    10681 taaattaccg ctttcatata tccaaccgga atccagttct atttccatac caaattcatt
    10741 tggagtagct ccgcttttga atgaagcaga atcaataatc aatacacctg acctgactaa
    10801 tgtttgcggt ccgactacat tattaacaaa agtgagactt ctgttaaccg gatctacaca
    10861 tccgcttaaa taaacatcaa agtttgtaaa tgtaacggga gttgccggcc agtcagcagt
    10921 agctgaaacg ggaattctga aagtaaaaga atttattcga gaatttatca aagctgtgat
    10981 ctgattagca tgaataagaa gctgataaga ccttggtgaa tttgctagtg gtcctaaaaa
    11041 tgtagctgta ccgggtaaat ttgaatatgc aggcgggact acattaaaat ctgaattaga
    11101 agtattaatt ggatcagggt aactattgga gttgttaaaa ataattaaag caaaacaaaa
    11161 aaatattaat aaagaaattt tgtgaaaatt tttcatgatc tcctccgtac ttgtttaatt
    11221 aataaaaatt tttataatct gattttactt aaaataatta atagttaatc aatgttcaat
    11281 atttaatttt aataaataat tgtatatgcg aatttatttt atttactatg ttatttattt
    11341 tgagatgaat tcaattccag caattaaaaa actaactatg gaaaaacctc gtccgttatg
    11401 ttagctaaga aaaaaacaga aagaacttgt aaatgcaaat gttgcacaca atgaaggact
    11461 ttcaaaatcg gatcgagtag cattctggat aactgatcat gtaagcagta agggattttt
    11521 atatttcttc tagtatggac tgctctatgg ctgggctata atatacatgc accaattgta
    11581 ctttatgtac cggcatttga tcctgcattt gtattgcggc ttttcatttc aaatatgaat
    11641 cagatatttt taatgcctct gataatggtc ggacagaatc tgcagggcaa aacattcaga
    11701 tgccagagca gaacctgatt atgagatcaa tcaaaatcgg aacgtgagat cgaagcaata
    11761 cttcagcatg ttagaaaatc aaaatgagct tataatgaaa attctaaagc atgttgatgc
    11821 aaagacataa cgggttttaa aaaaatggat ttagaattta agaataaaag aaaaaaattg
    11881 caaaaaatta tctttttgtc ttaatttatt cttaaatatt tattgcaaaa gagttaaaaa
    11941 atattaaaag aagcagtttg ttttttttgt attttatttc gaagttaaat ctgtaaatat
    12001 tttatattac atatagttag gtgaaaaaat acttaatatt tctctaacaa tttagtttga
    12061 aaattggtat atttcaattg gaaatattct aaataatatg taattgttta cacttcttac
    12121 tgaataacaa tttcatttta taaagcataa taagtaataa atattttcaa aaaatatttt
    12181 atatttctca atatgtgtct gtcaagatta aaaaataagc aaagtttaaa attgtattca
    12241 atatatgctg aataaagaat tatatgccag ggatatttcg ggtgatgtag tactgttgaa
    12301 aaaaatttct accggggata cagagtcatt aagaaaatta tataatcagt attcagatta
    12361 tttgtttaaa attgtttttt atattttaaa aaatgaatct gatgcagagg atactttaca
    12421 ggaagtattt atgcagatct gggagcattc agaaaaatac gatgaaaaat taggttcacc
    12481 gatatcttgg atagtaagag taacaagaaa taaatcaatc gacaaattaa gaagtaaagg
    12541 ttatagagaa agagaaaatc aatttgatat agacaggttt tacaatttaa gttcagatga
    12601 acccagtgaa aatccggatt cagtaatgac aattaattct acaagtgaac aaattaaatc
    12661 tgctatcgac actttgtctg acattcaaaa ggaacttctt gaatttacat atttcagagg
    12721 cttttctcaa agtgagctgg ctgaacattt tgaacttcct cttggtacag taaagacttg
    12781 tatcagaact gctatgataa agttaagaga taaactaaaa catttaatta attaaaaatg
    12841 gaaaataata ataatataat tgacgatcat ataattttca gatcacttgg aatggaattt
    12901 ggagaaattg attccgattc aggagtaaat gaaaataacg atcaaataca aaaaatcagg
    12961 gaatataatg aaattgcatc cttgattcct tctgtgctga acgatgtaaa tttcagcaat
    13021 acttttactt cgaaaaaaaa agaaagcatc tttgaaaatg ttttgtcaga gatagacaga
    13081 aaatcaaaat tagaaaaaaa gcatgagaat tttagttttg tttttgacaa taattcagag
    13141 tggatcaatt atccgataaa aggtataaaa tttaaaccat tatcattaaa caaagaaaaa
    13201 aattatttga tgcttctatt aaaagcagaa gccggatgtc agtatccatc tcatcatcac
    13261 agcggtgctg aagaatgtta cgttatagaa ggtgatcttt gtgccgaagg aaaagtactt
    13321 ggtccaggag attttcatca tgcagaagga gacagcgatc ataaatcatt atttaccaaa
    13381 aacggatgta cgctattaat tgtagctgat cctgaagatt atacataaat atttagtagt
    13441 taatatttag tagtcaatat ttaatagtta atatttagta gttaatataa aacacccatc
    13501 actatttttc acttgtcctg cccggacaat tttaaatcaa aaaaatttag caataaagat
    13561 ttgtattaaa cgaaggtgat tattctgaaa tcagaataat atcttttagg tgagtattat
    13621 ttactggaaa attctttcgg ctgcccagta gaatgcaatc agtgcaatga taagtgagcc
    13681 cggaatcaat atcctttttc tgtaccagct ctccgctctg aaccaaatcc cgactgtaat
    13741 aaatgcggct aaaactaccg cgatctgtcc gaattctact ccaatattaa atgagataag
    13801 agcatttaaa aattgttctt ttgggagtcc aaggtctttt aagacatctg caaatccaag
    13861 tccgtgaatt agtccaaata aaaatataac agtaattctg aatgaattta atttccttga
    13921 ataaatattt tctattgcaa taaatacaat cgataaagct atgataggct ctacaataaa
    13981 tccgggaata ttaacaatat taaaaacagc aagtgagagc gtaatcgaat gagcaattgt
    14041 aaatacagat atctgtatca gaagtggttt taatgacggg ctgaggagaa ataatccgag
    14101 tataaaaagt atatgatcca gaccgtaggg aataatgtga gtaaatccaa gctgaacata
    14161 aacccagccg gtatcaattg ttgacatatt tgccaggtca tgtatcggat gtgaaaaaac
    14221 aattccaggt agactgaaca aagccattaa agcggtaata aatattatat aaatattttt
    14281 gtttaagatg taatttatat taaacattga agtaatttta attttgaaca taaagtgatt
    14341 catagaacgg atttatattt aatgcaaatt tcagatattc atttgcctct gaatgtttac
    14401 cggttttttc gaaaattttc cctgcatgat aatataaaag tggatctttt gtaccaagac
    14461 gaagtgcctg actgatgtaa ttttctgctg catttatatt accggattta aaagctgtcc
    14521 atgcagctgc gtgaaaagtc tttatgcatc gtgaaccttc ctgcagagat atttctgaaa
    14581 tttcaaaaga ctcatccacc ctgattccat gatcagcatt aaaaagagcc agctcaagat
    14641 cagtttcgac tccattttgt ttgtaaaaag taattaagaa ttttactttg ttataggctt
    14701 cttcagcatt ttcaatctcc ccgtttaaag tataagcatc accaagagag atcagaaatt
    14761 caggaacaga atttttttga acagctttat tatacagttc aatagcctca ctatatttat
    14821 tataatgcat ttttatttta gccattcctg catatgcata tgaatattca ggataagcaa
    14881 aaattatctc ctgaaacaat tcagatgcag tgtcagtgtt tcctgcatta tacataagat
    14941 tagcaagctg atatttacac cagacagtgt tttccgcaac cggtgaacct gcattaacag
    15001 cggatctcat tgcttctata gatccttcag tgtcaccgaa taattctctg acataagaaa
    15061 ttctagaata ggaactaaga tcaggtctaa gatcaaccat tttctgaaga ctgataatgg
    15121 cttcgtcata cattccaagt tcaacctgag catcaactaa cacaccatat gaataagcac
    15181 tgtatggatt ttgagtaagt gatttttctg aaagttcaag tgcttccctg aagtgatgac
    15241 gggatagttt aactgaaccg agctctgcat aagccagaaa attttcctgt tgcagatcta
    15301 aggatctgtt caatacattt tcagcagatg catagtattc tggatcgttt gtttcacggg
    15361 ctttttggat ataagcagca ccgagtttgt ttaggatgtt tgtattttca ggagcttctg
    15421 tcaactgttc ctttaaaata gaaatgtatc tgtcagcgga agagttagta ttgctgatat
    15481 aattaatatc attatcataa ataccctgtg gcgaaacagt ctgttcagtg tttttgtcaa
    15541 agactttcca ggtaattaat gataagacaa ttattacaga ggatattata aatatttttt
    15601 tcatatgtaa aaaaattaaa aagcagtctg tcttacaggg acagactgca tattagaatt
    15661 attttaagag caacattttc tttgaatcta cacctgagga agttacaagc tttacaaaat
    15721 atgtaccgga agctactgca acgccgttat tattcattcc atcccacact actactcttt
    15781 cacctggtgc aagcttttca cttacaagag ttctgacttc ttttccaaga atatcataga
    15841 tcctgatgtc aacattatca gccttgagca gattatattt tatgttagtc gaaggattaa
    15901 atggatttgg ataattttgt atcaatccga atttttcggg gttaatattt tgattgctgt
    15961 ttgcagctat agaaacaatt gtaccgtgac tgttttcata accttcgctt gggatagcca
    16021 gatatggaaa agctgaagca aaggatttat catttctctg tacagcatcc ccgagctgag
    16081 catttatacc ggtactaaac ggaggtccta ataacacacc gccgaccact ctaagagcta
    16141 tgtcaattac atcatcttta agtcgtcttc catttgggaa tccggcaaca tcgccgtcta
    16201 ttacaccaag cctgttctca ttttcaaaag caacaggagg aataccaaga ttaattctta
    16261 attgctctga aggagttctt gtatcctgag gtctttgagt aagtcccggg acacctgtta
    16321 aaaaaactgc aacgagatca gttctgtttg tagtaggaat atcatcagtt acaggataga
    16381 gtgagttaat gatacctgca agttcaggtg aagtaacata attaagaaac tgaacatcgt
    16441 ctaccggctt tgaagcattc catttatctt tttgacttag aggaagtaca acttcattca
    16501 ccaatggcat accaagtctt gaaacaccga cccagtttcc gctaccggtt cttgtaccat
    16561 tgctgtttat tacagttgta gcttttctgc tggcagtagc ccatacacct acgatagcat
    16621 tggaatcatt ctgattagga tttggactac cgtttcttgt aacctgtgcg attgggactt
    16681 tgatcgagat tgagtgaaca ttatatccgg caagagcatc tttaccgccg ccaccgttac
    16741 caggagcacc cggtcttatt ttcaaaagat caaatgctgc accaagatcg acaaagaaag
    16801 gatcatctgt cggaccggca aaaacttttc cgccggagct aggaagatct cttacacctt
    16861 ctgacatgag tcctgcatag tttggcattg aagaaggtcc ggcataattt ggatcattgt
    16921 gagaatttcc accaacatag ttaggtgtga caggcagtgg agatgaagtc cactcggtaa
    16981 ctccgttaac aactctcttt actgtgtagg tttgttttac attcagatca ggatccccta
    17041 aggtagagac ttcaccagtg ttataaagga atgtatttcc atttcctact atatctgtaa
    17101 attcaaactg ataacttatg tgatcaattc tgtctccgac gttatcaatt ttaatttcat
    17161 acaaaacatc agtaccgaac ttatggaaat taggaccacc ggccggttcc tcaagaggat
    17221 tataacaagc gatcaggcag accattccgg gatcatcagc cggtctgaat gcatagacat
    17281 ctgtattgtc cgctactgga tctgtagaaa tgatcggtgc ttccctgtga ctggaagcgt
    17341 ctgtccattg tgacagacta aagagtgcag tgcaaactgc aagtactcca aacaaagagt
    17401 ataaaaattt ttttttcaac atttcagttt ccttttcttt taattattaa ttaagatgat
    17461 ttgtgttcaa aataaataac gaaataaaaa attgattgga tttaataaag aatgtaaaaa
    17521 aatcagagtg gaattatcgg aaaaaaatat tccgcatact tttcagttgt aatgcaattt
    17581 atatgatact ggtcatagcg gattatagta agttttatta gtttccgttt attaatgaat
    17641 tcaaataacg gaaaatatta taaaaaaatc tttttatatt attgccgttc tgattttttt
    17701 tgtgacaggt gtttccagat cacagatcca ttcagaaaaa ggcagttttt tatgttcaga
    17761 aaaaaaatct tcttcagatt acaatctgtt caaccccgac ttttgaggca aagaattttt
    17821 ctgaaaccag gaaaatgatc ctagtaaaat aatttttgta attaaccctc gtcagagttc
    17881 atttgatttt taagaactct gatgagaggt tgatatttta tgattcattt caaatcagaa
    17941 aactaataat taagaaattg aatgggtaat aatttccgta atgtgtaata aacccacagt
    18001 tatatttttt acattgaaaa acgaatagaa atagcacttt taaacggtat aagaattgaa
    18061 gttaaattca acataattta aaaacttaaa caatagaaaa atgaaaaaag catttctaat
    18121 ttcaatagtt acgtttgtga taatactaac aaacagtata ttggtttata catatgaaga
    18181 cgggataaca ggaaggacaa aaaagaccag tgcaaatgga tgttcatgtc attctatgaa
    18241 tacaaatata actggagttt tttccggacc tgatacagta tctgcaggtg agacagtggt
    18301 ttatagtttt acaataaaca gatccggctc cggagccggt gggggagcag atatttctac
    18361 acgacttggt gcgcttggag taggtcccgg agcttcttat ctcagaattc aaaacggaga
    18421 actgacacat caaagtccgt taggtttgtc aggcggaagc ttcacaggcc agtttaatta
    18481 tactgcccct tcatcacccg ggacagatac gctttggatc agtcctgcag ttggatatca
    18541 aaacggctgg aactggggta cagaaaaaag aatagtagta aaaaatcttg ttggaattaa
    18601 taatttaaac aatcctgtct attataaatt atatcagaat tttccaaatc cgttcaatcc
    18661 ttcgactaac ataagatttg aaatacctaa gtcagaattt gtcagtttga aaatatttga
    18721 tataacggga aaagaaattt cttctcttgt taatgaaaat cttcaggcag gcgtttacaa
    18781 tatcagattt gatcttaaca attcaattta tcatcagttg acaagcggaa tttattatta
    18841 catcttaaag actgagaatt ttactgagac gaagaaaatg atgcttgtca agtaaattat
    18901 aaattgtaaa ttgtaaattt ttatgcgcaa tgttgataca ggctatagcc tgtatcaacc
    18961 atagactgta tcaacaggca tttttataaa ttgctaccca tcttttatta aagtaaatta
    19021 ataatatttt ataagccgat tttttttata atggataaaa ttagcgaact tctttcacac
    19081 aaagaattta cttcagaatg ggaaaagtca aataaagata tcaatgccgc taagctgctg
    19141 actttaaggc aggttgaaaa attcgaagat atagtatctg atgatgtgag ctgggaaaag
    19201 cttttggaaa tatttaacag cggcagggag actgacgcct ggcttgcaat ggactggcct
    19261 gaaggatttg acgaactgat gctttgcgtt ccgttgtgta agcttgtaga ttttaaatgc
    19321 agtgaatgta ctgtcggtat gaggcagaga aataattcct gcgcaaatga tttctcttta
    19381 tttggataca ttgcggaatt actgaaagcg gctgacaggg aaggtctgaa agatcattta
    19441 ggttcagtga aaaagattct tctcctggaa aattttctat ggaatatcaa agagaaaagg
    19501 acagaagaaa taatttaatg atatttttat aaatttattt taataaaacc atacgcttag
    19561 tttcagaaaa tatttaggtt tgcatccgga tgtatcagat aattaatgta aagttaaccc
    19621 ccgattgaat taaaagcaaa gaattatttt tgcgtttaga ggggaattat ttttgaaaaa
    19681 tggtcaataa agttttgtaa aatattgatt atgagccgga aacaaagaaa aaagatggtt
    19741 ttatcccgga aaggatttgc tgtttgtgcg ggaaagtgca atttcaaatt acgaatttca
    19801 aatttcaaat agtgaaaaag tgtgggatta tgagtaacaa gtttcaagta tcgaatattg
    19861 aattctaaaa tatttatttg aaattcttaa tttgaaattc gtaattatta ctttatgtct
    19921 tttacctcag agatctcaag ttcataaaac tcatctcctt ttttcaattt ataggtgatc
    19981 ctttttttcc ctttagtatt gtcttcagcg aggtcggaag atttataaac aggaaattct
    20041 cttatgatat tttttccgga aaaatagatt gaatcattac ccaggtagcc tgccaagttt
    20101 tcatcttttt gatattcctc tgtagaagtg tcaaatgctt ttatttcggc aagggcagta
    20161 tcaccattgg aagcagctcc gaaaatattg aggaaagatt cagatcctgt agattttgta
    20221 ataatatata attcctcgaa accgtctcca tctatatctg caatgagcga gttatgcatt
    20281 ggatttttta aaggaaaaga caaagtgtct ttgaaattat taaatccaga tccagtaaca
    20341 tagtaattgc ttacactttc agttggttta gattcgataa ttataaactt tttacctgtt
    20401 ttggtttcat gttctttgta aactattaaa gtagtatctt ccttaactat catttccact
    20461 gaatcttttt ttacaaggat ctcaggtttc ttttcttctc cgcaggaaat aaaaatcaga
    20521 gaaaaaacaa tataaagtat aaatattttt atcataagta attaattttt ttgaataaaa
    20581 taagaaaatt atttctatga taaaattcaa tttaccttta taaactgtac agatatgtat
    20641 taggacattg aggaattatt ccctaaacat tcagaatgat tttatatatt tttgaactta
    20701 tggaaaatga atttaaaata ttagttgtag atgacgagcc gaattctacg aaacttatgc
    20761 gaaagatcct ttcggcaggc gggtataaag ctgaagagga gaacaacagt gtaaaagcca
    20821 aagaaaaaat ttccaccggt gattatgatc tggtaatatc agaccttcag atgccggaag
    20881 tatccggact tgatctgctg agatcgaaac cttcggatac taattttata atggttacgg
    20941 gattcggatc ggtggattcg gcagtagagt cgatgaagag cggtgctttt gattatctga
    21001 gcaagccatt taatatagat gaatttaaga tcaaagttaa aaaagcttta gacaatatta
    21061 agctgaatca acgtctcaaa gatctgaaga atgagataga taagaatcac aactttggta
    21121 atatgatagg aagaagtcga aagatgaaga gtgtatttga gatgataaaa aatgtatctg
    21181 agactaatgt aaatgttttg gtggaaggac agagcggcac aggaaaggaa cttgtatcga
    21241 gggctattca caataacagt ccccgaaaag ataatccatt catagcgatc aactgcagtg
    21301 cgattcccga aaatttatta gagagtgaac tctttggaca taccaaagga gcatttaccg
    21361 gtgcgactga aaatcaaaag ggagtatttg aacaggccaa tggcgggaca ttgcttttgg
    21421 atgagatcgc agaaatgcct tttcatcttc agtcaaaact tttgcgtgtg atcgagaact
    21481 gggaaataaa acctattggc agtgacaaaa ttaaaaaggt agatgtcaga ctgatctcag
    21541 caactaatca gaatataaat gaactgatac ttgaaaagaa attcagagag gatctttatt
    21601 acagaatatc aacagtgaca atagtaatgc ctgctcttac tgaaaggaag gatgacattc
    21661 cgcttttgac ggatcacatt ttgaaaaaac tttctgtaag atatgataaa aattattcaa
    21721 tagatgccga tgcacttgaa ctgctgatga atcataactg gaagggaaat atacgtgagc
    21781 ttgaaaatat gctggaaaga actgctctca gttctgccga taatgttatt aaaaaaagcg
    21841 atttcaggat ctcagacagc ggaggcggaa atatgaacga cagtatttca aatatcactg
    21901 gtggtctgaa ggagcttgaa aaaatttaca tcagtaaaat acttgatgaa aataaatgga
    21961 ataaacttaa agtatctcag atattgggaa ttgatcgcaa gacgctgtat aaaaaaatca
    22021 gggaatataa tcttgaatag acaattttat tcaaattaac ttcaaatttg tttcaccaac
    22081 atctaaaatc ctgctgattc ctgtcaatcc cgcagaaaat ttttacatat ccccgactgt
    22141 ttaacattag ttcaaaattc tacattgtcg ataatttccc cataaaaatg gtacataatc
    22201 tcactttttc caaatacact tcgtttttag tttaaaatta atagaaattt cacgtttcgg
    22261 tggcacattt attgacataa tattaatgaa tttaagtgtc cgaaattgat gaaaagaata
    22321 attcacataa tttgtataga tgacagtatt tgtcagatga ttcaaaagac tttgagatca
    22381 aagggatatg aagtatcaat ttcagacgga aataaacttt ctgtaaagaa tatcatcgaa
    22441 ttgagtcatg atattgaatg ctttgtgata gattcaggga ttgatgaagg cttgttttca
    22501 aatttgaaat taaaattttg taattcttca tacatttttt taccatcatt aaataaagac
    22561 agtaaaaaaa tcaacggaga ttatagtaat atatccgagc ctttaaaatt gagtgaatta
    22621 tttcagacat tggacagtgt atttaaagaa aaagagattg aaaattaata taaaatatat
    22681 cgcggcgttt ttaatttcat tatgtctgat aattttcatt tcgatctctg cggtagaata
    22741 tacaaccagt aagtccaaag atatcgatga ttgcctggct tgccatgagg atagtgaact
    22801 ccaaatggaa tcaggcacta aacaaatatc agtttttgta aatcctgaac tttataagaa
    22861 atctgttcat ggaagtgctg aatgttcaga ctgtcatttg aattataatc ctgacgagat
    22921 accacattca aaaaacagta aagctgtaga atgcagcacc tgccatgatg atctgaaagg
    22981 aattgagcat agtgttcatg caaagacaga gtgtgttgat tgtcacggca agcatgacat
    23041 aatgccggtt aaggaaactc aagctgagag ttctgcaaat tgtctgagtt gccataagaa
    23101 aaaatcaatt actcaattca gttcgagtat acattcaaaa aaaggagtga aatgtaatga
    23161 ctgtcacgga gacggacatc aggtaaaaaa gatttcaaga aatcagataa cagatttatg
    23221 tggaaagtgt cataacgaca gtaagatgaa tttcaaagga agtattcacc attctgcaat
    23281 aaaggatgga aataagaata agaacactcc tgtctgcact gactgtcacg gttcgcataa
    23341 gatagtgaca agcaaattca agatagaatc tgaagcatgt atgaaatgtc atttggatga
    23401 aaaacttttt ccgggcgagg aaaccggatc ggcaaagttt gtcgcgcaat ataaaacaag
    23461 tgtgcatgca tcaatcggga aaggcggtat ggaatctgcc ggctgtacgg attgtcacgg
    23521 agatcacatg atccagagtc cggatgatcc gaagtcttcg acaaagagag cgcagcttgt
    23581 cgagacctgc ggtaaatgtc atgctgatgt tgttgcgaat tttaaaaaat ctaaacacgg
    23641 aaaagaactt attaataata ataaagatgc gccaacttgt tctgactgtc atggcgaaca
    23701 taatatacag gcaacacttt catccgatga attttcaaag ctgaacattg caaataaatg
    23761 tattagctgt cacgaaaaaa gtaaacttgc tgaaaaaact aaggaaggta atgatgaaat
    23821 ggtaattgat tttaaaaaca gcgtacatta taaagcatta ttaaaagggg acatgagttc
    23881 ggcgacatgt tcagactgtc atggcgcaca tgagatggat aagccagacg atccgcattc
    23941 aagggtaaac agaaataata ttgtttctac ttgcggacag tcaaactgtc acagcagtga
    24001 attagcggaa tacagcggga gcgttcatga tatttcacag agtgaaaagg aaaacccgaa
    24061 tgtaccgact tgcaatactt gtcacggcaa tcacataatt cttaaagctg atgaaaagga
    24121 aaacaaactt tcatattcaa aaggaattgt tcagacttgt tccgattgtc acgattcgag
    24181 agtaataaat gaaaagtatg acataaagac aaaacaggta gagacgttta aggaaagcat
    24241 acacggaata tacattcagt ttggagatga gaaagctgca aactgcgcga gttgtcacgg
    24301 aactcacaat atccgcaggg aagatgatcc gcaatcaagt atacatcaat caaacatttc
    24361 aaagacatgc gggcagacag attgtcatcc gaatgcgagc gttaattttg taaaaggaaa
    24421 aatacaccta gatccaaagt cgaaggattc gggatttata tattatgtta gtacattctt
    24481 caaatatttt acgttcacaa ttttattttc tcttttctta tacatagtcc tagatttgag
    24541 aagtaaaata cgagaaagga aaagaccgaa atcagaaaat aatgatgaat agttctggat
    24601 caaatattga gacaaattca acagaatttc aaaggttcgg attaaatatc cggttacagc
    24661 atattatatt aatggtatgt acaattctat tgataatatc cggactgcct ttgaaatttt
    24721 atacaacgga ctgggcaaag tctgttataa atttgcttgg aggaactgac ggagcaagga
    24781 aggtacacca ttttgctgct cttggactga tagtaattgg gatttaccat ttattatttt
    24841 cgataataaa caggtcagga agaagagact ttatgctgct gatcccgggg aaaaaagata
    24901 tgaatgattt ttttcatcag ctgcagtatt tctttggtaa aaaaaagtac aagccgaaat
    24961 tcggaagatt cagtttcata gagaaatttg attactgggc tgtgtactgg ggatttatta
    25021 taatgatcgg ttcagggata ataatgtgga tgtttcaggg gaaattctat cccatgtttt
    25081 tcggggaagt ggatatatct tttacatctg gaatagtgga taattatgtg catgcgatat
    25141 ctcgtgaagc tcacagtgat gaagcattgc ttgctacact tgcaattttg atctggcatt
    25201 tttataatgt acattttaat cctgataaat ttccgggaag caaggtttgg ttcagcggaa
    25261 aaatgactag aaaagaaatg gaggaagaac atccactgga acttgaggaa attgaaaaca
    25321 gactgaataa aaaagaaatg aatattgaga aagacggaac tgtagtgagt gaaacagaac
    25381 aaactgaagt agatacaaaa gtttcagatg taattaagcc tgacacaaaa ccggacaatg
    25441 tttcaaatga tatatcagat cagaaaaaca atataactga cagcagcgat ggtaacgcaa
    25501 aaagtaatga caaaacatcc gggaccaaag atccagataa tgaaaaaagt aataaagtta
    25561 aataattttt ttaatcaagt actgaaaaat aaaatttact ttaaaatatt ttaaaaaata
    25621 aatcaataaa aaaatgaaaa agatatcgtt cattttgttg cttacagcag tatttgctgt
    25681 catagcaatt ttaattaaca tcgaaattac aaaatcgcag ccgcagtccc aggcgaagta
    25741 tgtaggagtg acgacttgct taggcgcctg tcataaaagc gagaaacagg gtaatcagct
    25801 tgaaatctgg aaaaattcaa aacattccca ggcattcaaa tcccttcaga caccatttgc
    25861 tgacagcatc gcaaatgcta aaggacattc aacagcagca gcagaaacac ctgaatgcgt
    25921 agtatgtcat gtacttggaa gagaaatgat gccgg
//
LOCUS       DAORVQ010000025        24839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_721_length_24839_cov_6.129196, whole genome shotgun sequence.
ACCESSION   DAORVQ010000025 DAORVQ010000000
VERSION     DAORVQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 24839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24839
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_721_length_24839_cov_6.129196"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLX80_03735"
     CDS             complement(<1..310)
                     /locus_tag="PLX80_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid delta-isomerase"
                     /protein_id="HQY19940.1"
                     /translation="MDVNRIAQGQLDAYNDQNIIEFLKFYSDDVEVFNFPDEILYKGK
                     EIMREKYISAWQQNPLQKAEVTERISLGNTVIDKEYVTGRKDGSIANVIAVYKIKEGC
                     I"
     gene            complement(345..764)
                     /locus_tag="PLX80_03740"
     CDS             complement(345..764)
                     /locus_tag="PLX80_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQY19941.1"
                     /translation="MNIENEDNFHKIILSKTYNANRNAILNLFKDGTIFKLTGADEIN
                     FDFCEGGKFLLVFNNRGKIFGSFIKITKDKLILDWNVEGFNRPCEINTLLDISFTQNG
                     NKCSLNLEHTKIFNKESAEAKNIAWREILNVIENKFG"
     gene            complement(876..1673)
                     /locus_tag="PLX80_03745"
     CDS             complement(876..1673)
                     /locus_tag="PLX80_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQY19942.1"
                     /translation="MKYFKDLYTSHKYFFNSVLSIFILLVLILNLFDLQFSGSKTSDR
                     LAPVWLFITAGGGVYGIAVSFLIFAFYVLFQTKKNHLYKRPVLFFLIIVFLVQLINAG
                     STYFIFKGSYNKMRPSQHFITGTEGPDKGEILFYEKLSKDKKKFLREKTLSEPERFLD
                     IYPPLLSDWVNESENSFPSGHSQSSFFIAVIFAFVISCLVKKNKIYLSVIPLLWALLV
                     SVSRVIIGVHYNIDVIAGAGMGISTALILISIKRFNRIFIVDEKQLS"
     gene            complement(1812..2105)
                     /locus_tag="PLX80_03750"
     CDS             complement(1812..2105)
                     /locus_tag="PLX80_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19943.1"
                     /translation="MNIFPEELNNYLKEYSTDPSAASCSDEKLIKHLKKYDAESDYVH
                     LDELNENQIFTLKNSGRFIKGKKRRKRFLCIEIGTGRKYLISPVAEVISETMF"
     gene            2199..2366
                     /locus_tag="PLX80_03755"
     CDS             2199..2366
                     /locus_tag="PLX80_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19944.1"
                     /translation="MSDLMDEYDQKEIFIEIMIYSNFVGLAFVRVCVVAELGIPGFGD
                     LKKEFVFIDPF"
     gene            complement(2437..3036)
                     /locus_tag="PLX80_03760"
     CDS             complement(2437..3036)
                     /locus_tag="PLX80_03760"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQY19945.1"
                     /translation="MKTEFKIKDKKVRCSWCLSSDLMVKYHDEEWGNPVHNDRKHFEF
                     ILLDGFQAGLSWSTIINKRKNFKKAFDNFDYKKIAKYDEKKLNELLIDEGIIRNKLKI
                     RSSVDNAKAFMAVQKEFGSFDKYIWQFTGGKTIHNNWEEMKHVQPTTKESDAMSKDLK
                     KRGFNFVGSTICYAYMQAAGMVNDHLKSCYRYKEVKKLK"
     gene            complement(3026..3538)
                     /locus_tag="PLX80_03765"
     CDS             complement(3026..3538)
                     /locus_tag="PLX80_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY19946.1"
                     /translation="MNENLQLMQYMLDDVRKVTLSGIKDISKEQLFEKPLGNEFPIGS
                     YLMHLAECEIFWLEVLSGITQPEDLKKRSYYDVWYDAFKDASPPIFAIEISEYHEVMN
                     EARKNLLDYMATMKDSELDEMVTFKSGSGERTFSKKWIIYHLIEHEAHHRGQMFMLMR
                     MAGMNKKNEN"
     gene            complement(3568..4860)
                     /locus_tag="PLX80_03770"
     CDS             complement(3568..4860)
                     /locus_tag="PLX80_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY19947.1"
                     /translation="MSNDLTEIKIKELIVSDMAENLIGSEIIKLAWQINDKIAAGQEI
                     YNLTIGDFDPEIFPIPVELKDLIKQAYDDHETNYPPGDGVNELRKVVSQYLYDRSGLE
                     YNMDEILITSGARPVIYSAYNTIVNVNDTVIFPVPSWNNNHYTHLTRSNAVQIETKPE
                     NNFMPTAEEIEPFIKEASLLSLCSPLNPTGTVFSENELAEICDLIVNENNRRGEFEKP
                     LYLLFDQIYWQLTFGETKHFNPVLINPAMKEYTIFIDGISKSFCATGVRVGWGFGPKK
                     VISKMKSIISHMGAWAPKAEQVALAKYYADYDKVDSFLTHIKKEIYDRLKAFYEGFRK
                     LKEAGFQVDAIVPQASIFMTVKIDLIGKTIKNGKTLNNSADVTNFLIEEAKIALVPFS
                     SFGSPGDSVWYRVAVGTCTIEQIGDIMNKLKLALERLD"
     gene            5089..7758
                     /gene="aceE"
                     /locus_tag="PLX80_03775"
     CDS             5089..7758
                     /gene="aceE"
                     /locus_tag="PLX80_03775"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HQY19948.1"
                     /translation="MAKDKNNNVRDDENKEWIESLDYIFQTQGPERVTEILRKLQIRS
                     QKYGIRIPFSANTPYINTIPVESQPVYPGSRELERRIKSILRWNAMAMVVRANRELTG
                     IGGHISTYASAATLYEVGFNHFFRGKDDPCGGDIIYFQGHAAPGIYARAMLEGRITEK
                     DLNNFRRELSSDRGLSSYPHPWLMPKFWQFPTVSMGLSPIMAIYQARFNRYLEDRGVL
                     KKSDQKVWAFLGDGETDEPESLGAITLASREKLDNLIFVINCNLQRLDGPVRGNGKII
                     QELEAIFRGAGWNVIKVIWGGDWDPLLAKDENDKLVEVMNSVVDGQFQKYTVSSGEYI
                     RKDFFGRDPELLELVKSYSDEQLKKLKRGGHDPDKVYTAYKSAVTHKGSPTVILAQTI
                     KGYGLGESGEGKNITHQQKKLNEQELREFRSRFGIPISDEEVADAPFYRPAEDTEEIN
                     YLKKRRKVLGGFVPQRDGESAPSLKMPGSDIFKEFYDGSGQRVVATTMAFVQMMTKLL
                     KDKDIGKNIVPIVPDEARTFGMEALFRQVGIYSHSGQLYDPVDKESLLYYKEEKDGQI
                     LEEGITEAGSMSSFIAAGTAYSTHGINMIPFFIFYSMFGFQRIGDLIWAAADCRTRGF
                     LVGGTAGRTTLSGEGLQHQDGNSHLLALPVPNLKCYDPAFAFELAVIIEDGLKRMYEK
                     QEDIFYYITVMNEQYEMPSMPSGVRENVLKGMYRFSKSKSKDQKLKAHLMGSGSILNE
                     TLKAQKILEKEYGVSTDVWSVTSYKELYFDCIETERKNMVNPDKAKESYLTKLLSKET
                     GVFVSASDYVKALPSTISKWIPGSFYILGTDGFGRSDGREQLRNFFEVDHRYIIIATL
                     YSLYKEKKIKVAVVNKAVKNMGLDPKKPNPLEV"
     gene            7805..>8199
                     /locus_tag="PLX80_03780"
     CDS             7805..>8199
                     /locus_tag="PLX80_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine acetyltransferase"
                     /protein_id="HQY19949.1"
                     /translation="MIKEIKLPEISENVEHGDVIKILVKVGDNITKDQSIIELETDKA
                     TFEVPSTEEGIIKEILIKEGDVIDIGAVIIKVETNSKNGSSESENNDNSNKENGNKEK
                     DNDDEGKAEEKVKESESSETDDESKKIKSS"
     assembly_gap    8200..8299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8300..9308
                     /locus_tag="PLX80_03785"
     CDS             <8300..9308
                     /locus_tag="PLX80_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQY19950.1"
                     /translation="DKESSDKESSDKESSDKESSDKEEIVRSGDNINLLEKIKNKDLA
                     PASPSVRRLARELGVEVGDVTGTGISGRISEDDIKLYVKSLMKESGSISITSKALPDF
                     SKWGEISKEPMSKVRSITAESMTYAWTTPMVTQFDKADITLMEEFRKKYAKAVESKGG
                     NLTITSLLVKICTEALKKFPQFNSSIDVNKKEIIYKKYYNVGIAVDTDRGLLVPVIRN
                     TDKKNITEISVEMNDLAEKARTKKITPDEMNGGNFTISNLGGIGGTAFTPIVYSPQVA
                     ILGVSRASREAVFINENFEPRMMLPLMLTYDHRIIDGADAARFLRWVCQALENPMTIL
                     L"
     gene            complement(9350..10099)
                     /locus_tag="PLX80_03790"
     CDS             complement(9350..10099)
                     /locus_tag="PLX80_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQY19951.1"
                     /translation="MNNNNTVNNFRNYIPAAIVLAAGFIIGAFIISSTWKYVSRKDVT
                     INVTGSASKDIKSDYAIWNGSISIEAVSLQDSYAKLKEANEKVKNYLIGKGFPADKIT
                     VSAINTTTLYVSNDQGYATNQVTGYRLNQDFRVESEDVEKIDKLSREATELINEGIEI
                     NSPTPEFLYTKLSDLKVEMTGLAAMDAKVRAEQIANSTENEIGEVRSSRMGVVQINAK
                     NSNEISDYGINNTTSIDKTIRVVVNMSFSID"
     gene            complement(10105..10536)
                     /gene="arfB"
                     /locus_tag="PLX80_03795"
     CDS             complement(10105..10536)
                     /gene="arfB"
                     /locus_tag="PLX80_03795"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQY19952.1"
                     /translation="MSSIELNKEELRNELVFKTSRSGGKGGQNVNKVETKVELNFDVL
                     NSKVLSNIERELISKKLFKKLTSDGILKITSQTSRSQYMNKEEAVEKFFKIFEKALTP
                     EKKRKDTKPTVAVKEKRIRLKKQRSEKKVSRKFNPDKEINL"
     gene            complement(10533..11948)
                     /gene="lpdA"
                     /locus_tag="PLX80_03800"
     CDS             complement(10533..11948)
                     /gene="lpdA"
                     /locus_tag="PLX80_03800"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQY19953.1"
                     /translation="MSKRKLIVIGAGPGGYAAAFLAADLGIETMLIDKDVNPGGVCLY
                     RGCIPSKALLHLAKIISEAEESKNFGVNFSKPKIDLKKIRTWKNDVVSKLTSGLGQLS
                     KQRKIEYIQGTATFLDKNSIELTNTKNKKEKIEFEYAIIATGSYPTRIPALSDSSKNI
                     LDSTTALDLNKIPKTMLVIGGGYIGLELGTVYSALGTKVTIAEMLPGLLNGADRDLVS
                     VLSTKVNKKFESILLNTKVIDIKEIKSGFKVKLQSKENKVSEKIYESVLISAGRKPNS
                     SDLGLENAGINISKNGFIITDNQTRTSVNNIFAIGDVAGEPMLAHKASHEGRVAAEII
                     AGHKSAFEPKTIPAVVFTDPEIAWCGITENEAKEKGLDYTVSKFPWAASGRALTIGRT
                     DGLTKLIIDNKTERILGVGIVGPNAGDLISEGALAIEMGANATDLKLTIHPHPTLSET
                     IMESAEVFFGQSTHIYKPKKK"
     gene            12124..13335
                     /locus_tag="PLX80_03805"
     CDS             12124..13335
                     /locus_tag="PLX80_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19954.1"
                     /translation="MKLIKYFLIALFVTTSVQAQNVKITDFDIPVSSARKFLINGFYN
                     WAQTDPNDSALNLSSSWQLDGAYTQFYTSPSYAWHLNLTGSLNQLTREDTTRYNYNLA
                     TDLSKYFSDSHGWFGNAEVSSNYARKREFGTDNRPTIEIFGGLGYGRQVNATSLAKAV
                     RIDEDLRKGGITNKYMPKSTVLAIAQIVDRESEYRDKYKALYESKIIEDISTEVMNSG
                     VTNNTSMSALGFMRIRDVLYGLNQFINDRTYGGDIRLGVSYQVLTRNKELTGAPAFLS
                     LRGRYGYPIGLSHQISAFANAGTALDSAFGKLFTGNAGFAYSYNMTNRVSFNAGYTVT
                     FNQIFDQTNINNVGVPFGTDKSGGDNTATAGFTFYIENYITLNLTGGYSTIYGTEQRW
                     FSNASLGLIVF"
     gene            complement(13430..14248)
                     /gene="dapF"
                     /locus_tag="PLX80_03810"
     CDS             complement(13430..14248)
                     /gene="dapF"
                     /locus_tag="PLX80_03810"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQY19955.1"
                     /translation="MVIQKYSGAGNSFVINDNRNHETIEYENKVEFLMENHPGMDGVI
                     FLENSEKADFKMNYFNRDGTGDALCGNGLRCTVQYIADNKISDKKELNIEAVSEIYEC
                     KIISENEIAVKFPPPLTVKTNFKLKVHFQEWWDLLNCSYVEVGSPHIVVFIDDIEKPK
                     VKSIDEINISEWGRNIRMHKDLMPQGANVNFVEIIKKGELKIRSYERGVEGETLACGT
                     GSISSALIYYMTREQFKPVKLLTRSGESLIVDFEIDGKKLLNVILTGGAEKITF"
     gene            complement(14248..16875)
                     /locus_tag="PLX80_03815"
     CDS             complement(14248..16875)
                     /locus_tag="PLX80_03815"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HQY19956.1"
                     /translation="MKYIFFITLFIFSVNICNAQTITDKADSLENTDSLDIIKSDTFL
                     ENTDSLNSKVIGSLDSANVNSFDSSRTAKSDIDAIINYTGSDSIVYDLKNKKVIIYDQ
                     GILTYKDLRLEAGKIIVDQETQILEAFGIPDSANPEKAMQMPVMTQGSDKYEGTKLFY
                     NFKTQQGSVSEGFSEAEIGYYSGEKIKKESSDVLFIKNGIYTTSSDREDPEYYFLSPE
                     MKVIPNDKVIAKSVFLYIEGVPVLWIPFGVFPNKSGRTSGLIMPTFGDDATYGKYISR
                     LGYFWAVNDYMDIGFTGSYFTKGRTDIYGRYRYASKYNFSGTIDGGYSKIRLGESTDI
                     GNQNSDAWGLNVVHNQQFNPTTRLDASIQIASSKNFYNVSTNALSELLLQNLISNFTL
                     SKAWEGKPFALSMNYYRDQNLQTGDVTQRLPLINFSVSQTFPFESGFSDPFNKKLYEY
                     IFFSYNGSILNNSIKRTITDAAGNDSTYTDLRSGVRNSINIGLSPKFQYISLTPFFNY
                     NEIWYNKYVSKNFNPADSSVTTNDNNAIKSLRYFNTGVSLNTKFVGIFLPKIFNVTGI
                     RHTITPTITYNYQPDFSDPGWGYYQSYTDANGKEIKYSIYEREIFGGAPSGESQSIAF
                     SLGNVFEMKTRVNDSTENKFQLLNIDAGAAYNFVADSLNFSEILLSYRTSIGNFLNIS
                     GGTSFNLYKFDQTANTRINQFLVNTDGVLANITNFTINLSTGFSFAPEPTTSGVSEDI
                     TESDTLTTRVDYKDFRKDKLDEIDFQIPLSGGFGFNYSESKFNPAIVNRTSNLTGNLS
                     LNLTQKWRFTVSANYDLVNKQIVTPYITAYRDLNSWEMNFNWYPTGTYRGFSFEVRVK
                     APQLRDIKVTKKTNTRGVY"
     gene            17096..17797
                     /locus_tag="PLX80_03820"
     CDS             17096..17797
                     /locus_tag="PLX80_03820"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQY19957.1"
                     /translation="MKIDLIYEGKAKKIYSTDREDVYLQEFKDDATAFNGVKKDKISH
                     KGEINNFISTEIFKYLRENTIPVHFIEKISETEMLVSKVDIIKVEVVCRNIAAGSLVK
                     KYGFTEGEVLEKPIIEFYLKSDELGDPLFSEEHIYAMKLADKHDIDMIRKMTTYINTL
                     LQKFFLEKGIKLVDFKLEFGKDKHGNIILADEISPDTCRLWDSETDKKLDKDRFRFDL
                     GEVEEAYQEIKNRLS"
     gene            17797..18468
                     /locus_tag="PLX80_03825"
     CDS             17797..18468
                     /locus_tag="PLX80_03825"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HQY19958.1"
                     /translation="MKFTRYGKDVILKVFILGLVLTVLSLFIDNIIVKFLFTGITIFL
                     WLFTLYFFRDPDRSLPNGYKENQLISPADGKIVVIEDVINKEDNLFKKDEPLKQISIF
                     LSPLNVHVNRNPINGKVTYLKYIEGDYLVAFDHKSSERNERTEIGIENPENGCKVFFK
                     QIAGFVARRIVYDLKVGCTATAGDRFGMIKFGSRVDILIKRDSKILVEMDQIVIGGET
                     IIAEL"
     gene            18469..19224
                     /gene="pssA"
                     /locus_tag="PLX80_03830"
     CDS             18469..19224
                     /gene="pssA"
                     /locus_tag="PLX80_03830"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQY19959.1"
                     /translation="MFISRKFIPSLFTILNAFCGMMSVIYSAKGEFDEAALFIIYASL
                     FDAVDGLAARLTNSASKFGVELDSLSDVISFGLAPSILIYYVYFNQLGDIGIVMSSLI
                     MVFAAIRLARFNVQLTGFEKEKFNGLPAPMTAFTICTYILFYHNNIFSESVSEIFIIS
                     LAFFLPVMMVSRIKYDSIPKPSLHSLKKNPLVFIIIFIGIILVIATEGEGSFSICLFY
                     IFSGIIRTGYKTFFGKHKKSDDVNESLEFNKSK"
     gene            complement(19230..20384)
                     /locus_tag="PLX80_03835"
     CDS             complement(19230..20384)
                     /locus_tag="PLX80_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQY19960.1"
                     /translation="MEKIYLDNAATTKIDEEVFDAMLPYLKNYFGNASSIHSYGKTAK
                     VMLEDARDVLAEFTGVSSKEIVFTNSGTESNNHAIKGTAFRHIGTEKNHIISSTVEHS
                     AVLDTLKYLEERFNFDVTYIKPDRFGRIGTEEVLESIRPETFLISIMHSNNETGAITD
                     IKSLTESILEKDIYVHSDTVQSLGKTDLNFKDININFATISAHKIYGPKGIAVLYIKD
                     KTIVDKFIHGGKQERDLRGGTENIAAIAGFKKAIEILKRRMTEDVLHYTELKNILTSG
                     LNENFDKGIIYNSDTENCLQNIVNISFGKKFLEYDEEMLLIKLDLKGIAVSGGSACTA
                     GTLKPSYVLKELGHDERTALGSIRISFGRSNTLNEIEKLLEVLKENVKKK"
     gene            20605..21159
                     /locus_tag="PLX80_03840"
     CDS             20605..21159
                     /locus_tag="PLX80_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQY19961.1"
                     /translation="MPINKEYFYISNLLSVLRIVLLIPLSWLMLTEFDTKNNLIIILV
                     LCMYLSDLLDGYLARKLNEVSEFGKIIDPLADKICVIAIAVILVYLDRLPLWFVSVVV
                     LRDITILIFGVYLKRKKDITLMSNYPGKIAVFCIGLVLLFSIIDSESLKMISTIMYFV
                     SILMIAYSSHLYLKRYLETVRKPV"
     gene            21169..21522
                     /locus_tag="PLX80_03845"
     CDS             21169..21522
                     /locus_tag="PLX80_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY19962.1"
                     /translation="MKEKIKTYTRKDIEHRLGDKLAGKQLTEKLLVDSLFECLREVLL
                     EADPVSRLEIRDFGVFEIKKTKPKPKARNMQTGEFIFVPGRRKIHFKPGKIIRDFLKE
                     DYDEIIIKGTNDKDN"
     gene            21540..21983
                     /gene="ruvX"
                     /locus_tag="PLX80_03850"
     CDS             21540..21983
                     /gene="ruvX"
                     /locus_tag="PLX80_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQY19963.1"
                     /translation="MNTKYMGIDYGEKRVGISVSDESKKFSFNRDYIFNDKDFFKNLT
                     KLIIAENVSNIVIGYPVKLNSDRTEQTDNVDKFAEDLRKYLESNNVKAELAFFDERLT
                     SKIAAESLLRSGIKKMKRREKGLVDGISAQLILQDYLDKMNYSGR"
     gene            22033..22674
                     /locus_tag="PLX80_03855"
     CDS             22033..22674
                     /locus_tag="PLX80_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY19964.1"
                     /translation="MYLIELHEMIEGGENENVEFKRKFSSPEKIAKEMIAFANSKGGK
                     MIFGVDDDKSIVGVESEKGELELISTAARHYCEPEIEFKTDIMLLRNKDLVVVTIEES
                     KIKPHRLISDDDEKSEKVYIRFKDRSMLASKETVKILRNSNADAHPVNITVGDIEKTL
                     FDFLNKNEKITVKGFKKLVNISERRASRTLVNLVKAKVIRHNFMGKEDYYTLI"
     gene            23052..24722
                     /locus_tag="PLX80_03860"
     CDS             23052..24722
                     /locus_tag="PLX80_03860"
                     /inference="COORDINATES: protein motif:HMM:NF020683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1929 domain-containing protein"
                     /protein_id="HQY19965.1"
                     /translation="MPPSQGEWNILKVTINNEEVDLDSQVTAIHAHLIPVPTGSESHP
                     KGKVLYFHARYPSNNVNFEGEPTPNFVSFLFDPETNEMTQFTIPRFPDDIGVDVDPSK
                     LFCSGHTFLADGKLLVAGGERNQPPWGSNGTKYSFLFDPSVLGDTAPFPWKFTGGSTV
                     ITITKMSRGRWYPQLTKLHDGTVLAMSGYPETPPPPQDPPEITVIIPERYNPDTQEWT
                     PYTHMEDGLRIPLYNGAYVIPFGAWQGEIFYDMAAFGPQLEEWNGAHRFNPFGTTDLW
                     NPVGSQSKSVRLHGCSVLLPIKSTDTDIRIVNMGGFDDNSKSCELIEIGSNTSTDWVP
                     ITDLEHHRHDCPNALLLADGSLIIIGGNETLIPEMLDYSDPDPSNWEWSSLPAMEVPR
                     KYHSTALLLPDGTVWCGGSRIYTDPLKFEFENDMERRIEIYRPGYLLEGPGPVITSAP
                     LVINYDDVFEVGYNITAEPSPEIDSIVLISLPSVTHCYDSNQRYLILDFGSSVNFGAL
                     NVTAPIDEYLAPPGYYMLFLLQNKTQSISGEVRIPSIAKIVKLFRPSP"
BASE COUNT         8142 a         4141 c         4207 g         8249 t
ORIGIN      
        1 agatacaacc ttctttaatt ttataaactg ctatcacatt tgcaatactt ccgtcttttc
       61 ttccggttac atactcctta tctataactg tatttcccag actaattctt tctgttactt
      121 cagctttctg caaaggattt tgctgccatg cagatatata tttttcacgc ataatttctt
      181 tacccttata aagtatttca tcggggaagt taaatacttc tacatcatca gaataaaatt
      241 ttaaaaactc tattatattc tgatcattgt atgcatcaag ctgtccctgt gcaattctat
      301 ttacatccat aatttaaagt ttagcaatta ttcattttaa aaagttaacc aaatttattt
      361 tcaattacat taagaatttc tctccatgca atattttttg cttcagccga ttctttatta
      421 aatatttttg tgtgctctaa attcaaactg catttattac cgttttgtgt aaatgaaata
      481 tccaataagg tattaatttc acaaggtcta ttaaaccctt ccacattcca gtcaagtatc
      541 agtttatctt tagttatttt tataaagcta ccaaagatct ttcctctgtt attgaaaaca
      601 agcagaaatt ttcctccctc acaaaagtca aaattaattt cgtcagcacc ggtaagttta
      661 aagatagttc cgtctttaaa taagtttagg attgcatttc tatttgcatt ataggtcttt
      721 gataaaataa ttttatgaaa attatcttca ttctcaatat tcattataat tttttgttaa
      781 tatatttttt taaaaatgaa ctattgctta ctatatcaca aacaagaaat gcttttacaa
      841 acagattaaa attttgaagt acaattatac ggtttttatg ataattgttt ttcatcaaca
      901 ataaatatcc tgttaaatct ttttattgaa attaaaatta aagctgtaga aattcccatc
      961 ccagctcctg ctatcacatc aatattataa tgaacgccga ttatcactct ggacacagat
     1021 acaaggagtg cccataataa aggtatgaca gataaataaa ttttattttt ctttactaaa
     1081 cacgagataa caaatgcaaa tatgactgca ataaaaaatg aagattgtga atgccctgac
     1141 ggaaaagaat tttcggactc atttacccag tccgaaagta aaggcggata tatatccaaa
     1201 aatctttcgg gttcagacaa tgttttttct ctgagaaatt tttttttatc tttagatagt
     1261 ttttcataaa acaatatttc acccttatcc ggaccttctg taccggttat gaaatgttgt
     1321 gatggtctca ttttattgta tgagcctttg aaaataaaat aagtagaacc tgcattaatt
     1381 aactgtacaa gaaatactat tattaaaaaa aataaaacag gtcttttata taaatgattt
     1441 tttttggttt gaaataaaac ataaaatgca aagattaaaa atgaaactgc aattccatag
     1501 acacctccgc ccgctgttat aaaaagccat acaggtgcca gccggtcact ggttttactt
     1561 ccgctgaatt gaagatcgaa cagattcaaa atgagaacga gcagaataaa tattgataaa
     1621 accgaattga aaaaatattt gtgagatgtg taaagatcct taaagtattt cataactatt
     1681 tttaataaag aattatttta atcttactaa aacattgtct ctgagataac ttctgcaaca
     1741 ggactgataa gatattctat gactattgtt ctaggagagt cacgtgctct ccggactctg
     1801 cggttactga attaaaacat tgtctctgag ataacttcag caacgggact tataaggtat
     1861 ttccttcctg ttcctatctc aatacagaga aatctctttc tgcgtttttt acctttaatg
     1921 aatctgccgg aatttttcaa agtaaagatc tgattttcgt taagttcatc aaggtgtaca
     1981 tagtcagatt cagcatcata ttttttcaga tgcttaatca atttctcatc actgcagctg
     2041 gccgcagaag ggtctgttga atattctttc agataattat tcagctcttc aggaaagata
     2101 ttcaattcaa taaaaaaatt cagatgtttc ccaaactctg atttccattc cttgccgtga
     2161 ggtgcaacct taccgctaaa tttcagatag catgtgagat gagcgatctc atggacgagt
     2221 atgatcagaa agaaatattt attgagatta tgatttacag taattttgtg ggacttgcct
     2281 ttgtaagggt gtgtgtagtc gccgagcttg gtattcctgg ctttggtgat cttaagaagg
     2341 aatttgtatt catcgatcca ttttaaaata agatctactg atccctcggg aatatattta
     2401 cttaagatta ttttgttact ctcggaaata aaattattat tttaattttt taacttcttt
     2461 atatctgtaa caacttttta gatgatcatt aaccataccc gctgcctgca tatatgcata
     2521 acaaattgtc gagccgacaa agttaaatcc acgcttcttc agatctttgc tcattgcatc
     2581 tgattcttta gtagtcggct gaacgtgttt catttcttcc caattattgt gaatcgtctt
     2641 tccgccggta aactgccaga tgtatttatc aaaacttcca aactcttttt gaacagccat
     2701 aaatgctttt gcattatcaa ctgaagaacg tatcttaagt ttatttctta taataccttc
     2761 gtcaataagt aattcattta acttcttttc atcatacttt gcaatttttt tataatcaaa
     2821 attatcaaaa gccttcttaa aattttttct tttattaata atggtcgacc agctaagtcc
     2881 tgcctgaaat ccgtctaata aaataaactc aaaatgcttt ctgtcattat ggacaggatt
     2941 tccccattct tcatcgtgat attttaccat caggtctgat gagaggcacc aactgcatcg
     3001 aactttttta tcttttattt taaattcagt tttcattttt tttattcatt cctgccattc
     3061 tcattagcat aaacatttgt ccgcggtgat gagcttcatg ttcgataaga tgatagatga
     3121 tccatttttt tgaaaaagtt ctttctcccg aaccggattt aaaagtaacc atttcatcta
     3181 gttcactgtc cttcattgta gccatatagt caagcagatt ttttcttgct tcattcatta
     3241 cttcatgata ttccgaaatt tcaattgcaa atataggagg tgaagcatct ttaaatgcat
     3301 cgtaccatac atcataataa gatctctttt ttaaatcttc aggctgcgta atgcctgaca
     3361 gcacttcaag ccagaagatc tcacattccg ctaaatgcat taaatatgat ccgataggaa
     3421 attcattgcc aagaggtttt tcaaataatt gttctttact tatatcttta atgcctgaaa
     3481 gtgtaacctt tctaacatcg tcgagcatat actgcatgag ctgcagattt tcattcatat
     3541 aatttttact ttagattttt aatataatta gtcaagtctt tcaagagcta acttgagctt
     3601 attcattata tctccaattt gctcaatagt acatgtacca acagcaactc tataccagac
     3661 agaatcacct ggagatccga atgatgaaaa cggtaccaga gcaattttag cctcttcaat
     3721 aagaaagtta gtaacgtcag cagaattatt cagggtctta ccatttttaa ttgttttccc
     3781 aataagatcg atctttacag tcataaaaat tgaagcctga ggaactatgg catccacttg
     3841 aaatccggct tcttttaatt ttctaaatcc ttcataaaaa gcttttagtc tatcataaat
     3901 ttctttctta atatgagtaa ggaatgaatc aactttatca taatctgcat aatatttagc
     3961 aagagcaacc tgttcagctt ttggagccca tgcacccatg tgtgaaataa tagatttcat
     4021 tttactgatc actttttttg gaccaaatcc ccaacccact cttacgcctg tagcgcagaa
     4081 agacttgctg ataccgtcaa taaaaattgt atattctttc attgcaggat ttataaggac
     4141 aggattaaaa tgtttagtct caccaaaggt aagctgccag tagatttgat caaacagcag
     4201 atagagaggt ttttcgaatt cacctcttct gttattttca tttacaataa gatcacagat
     4261 ctccgcaagt tcattttcgg aaaatacagt tccggttgga ttcagcggag aacataaaga
     4321 aagaagtgaa gcttccttta taaacggctc gatctcctca gccgtaggca taaaattatt
     4381 ttcaggtttc gtttcgatct gaactgcatt tgatctggtc agatgagtgt aatgattatt
     4441 attccatgaa ggtaccggga atatcactgt atcatttaca tttacaatag tattgtaagc
     4501 agaataaata actggtctgg caccgcttgt aatcagaatt tcatccatgt tatattccag
     4561 tccggatctg tcatatagat attgcgaaac tacttttcgt aattcattta caccgtctcc
     4621 cggaggataa ttggtttcat gatcatcata tgcttgttta atcagatctt tcagttctac
     4681 aggaatagga aaaatctccg gatcaaaatc cccgatggtc aggttgtaaa tttcctgacc
     4741 tgcggcgatc ttgtcgttta tctgccatgc aagtttgata atttcagaac caataaggtt
     4801 ctcagccata tcagaaacta ttagttcctt tatttttatt tcggtaagat catttgacat
     4861 aattacaaat taattttagg gatatttgga ataaatggaa tattaagaat atagttaaat
     4921 gaaattttaa atttgatgaa atttttattt ccccatataa acgtgaaatg tcacaataaa
     4981 tatattaaac aggtaataat aattttatat acattaacag taaaattttt tcactaaagt
     5041 ctttgatttc aaatagttat ttttatactt gatataatat aaaattaaat ggctaaagat
     5101 aaaaacaaca acgttagaga tgacgagaat aaagaatgga tcgaatctct ggattatatt
     5161 tttcagacac agggaccaga acgtgtaaca gaaattttaa gaaagttaca gataagatct
     5221 caaaaatacg gaataagaat cccattctca gctaatactc catatataaa tacaatcccg
     5281 gtggaatcac agcctgtata tccgggcagc agggagttag aaagaaggat caaaagcatt
     5341 ctcagatgga atgcaatggc aatggtggta agagctaaca gggaattgac cggcattggc
     5401 ggacatatat ctacttatgc atctgctgct acactgtatg aagtcggttt caatcatttc
     5461 ttcagaggaa aagatgatcc gtgcggcggc gacattattt attttcaggg acatgcagct
     5521 ccgggaatat atgcaagagc aatgctcgaa ggaaggatca ctgaaaagga tcttaataat
     5581 ttcagaagag aattatcttc agacagagga ttatcatcat atccgcaccc atggctgatg
     5641 cctaaattct ggcagtttcc gactgtatcc atggggctta gtccgatcat ggcaatctat
     5701 caggcaaggt ttaacagata tctggaggac agaggagttc tgaaaaaaag tgatcagaaa
     5761 gtctgggcat ttcttggaga tggtgaaaca gatgaacctg aatcactcgg agcaattacg
     5821 cttgcctcaa gggaaaaact agataatctg atctttgtaa tcaactgtaa tcttcagagg
     5881 cttgatggtc cggtaagagg gaatggcaag atcattcagg agcttgaagc aattttcaga
     5941 ggagctggat ggaatgtaat caaagtgatc tggggaggcg actgggatcc gctgctggct
     6001 aaggatgaaa atgataaact tgttgaagtc atgaattcag ttgtagacgg acaatttcaa
     6061 aaatatactg tgtcaagcgg tgaatatatc agaaaagatt tttttggaag agatcctgaa
     6121 ttactggaac ttgtaaaaag ttattctgat gaacagttaa aaaaactcaa aagaggcggt
     6181 catgatcctg ataaggtata tactgcatat aaaagcgccg tgactcataa aggatctcca
     6241 acagttatcc ttgctcagac aataaaaggt tacggtcttg gtgagtcagg tgagggaaaa
     6301 aatattacac atcagcagaa aaaacttaat gaacaggaat taagagaatt cagaagtcgc
     6361 tttggaatcc cgatctcaga tgaagaagta gctgatgctc ctttttacag acctgctgaa
     6421 gatactgaag aaataaatta tcttaaaaaa agaaggaagg tattaggcgg ttttgtacct
     6481 caaagagacg gtgaatccgc tccttcttta aaaatgccgg gtagtgatat tttcaaagaa
     6541 ttttatgacg gcagcggaca aagagtagta gcaacaacta tggcttttgt acagatgatg
     6601 accaagcttt taaaagataa agatattgga aaaaatattg tgccaatagt tccggatgaa
     6661 gcaagaacat ttggaatgga agcacttttc aggcaagtcg gtatttattc acattcaggt
     6721 cagctttatg acccggtcga taaggaaagt cttctgtatt ataaagaaga gaaagacggt
     6781 cagatacttg aagaaggaat tactgaagcc ggctcaatgt catcgttcat tgctgccggt
     6841 acagcatatt cgactcacgg aattaatatg ataccgttct ttatttttta ttccatgttt
     6901 gggtttcaaa gaataggcga tctgatatgg gctgctgctg actgcaggac aagaggtttt
     6961 cttgtgggtg gaacagcagg aaggacaacg cttagcggtg aaggacttca gcatcaggac
     7021 ggaaacagtc atttgctggc actcccagtt ccaaatctaa aatgttatga tcctgcattt
     7081 gcatttgaac ttgcagtaat tattgaagac ggactgaaaa gaatgtatga aaaacaggaa
     7141 gatatttttt attacattac agtaatgaat gagcaatatg aaatgccatc aatgccgagc
     7201 ggagtccgtg aaaatgtttt gaaaggtatg tacagatttt caaaatcaaa atccaaagat
     7261 caaaaactca aggcacattt aatgggcagc ggatcgattt taaatgagac attaaaagcg
     7321 cagaagatcc ttgaaaaaga atatggagta tcaacagatg tttggagcgt tacgagttat
     7381 aaggaattgt atttcgactg cattgagact gaacgtaaaa atatggtaaa cccggataaa
     7441 gctaaagaat cctatctgac taaattactt tcaaaagaaa caggagtttt tgtttctgct
     7501 tccgactatg taaaagcact tccttctacg atatcaaaat ggataccggg aagtttctat
     7561 attctgggaa ccgatggatt cggaaggagt gacggcaggg aacaattaag aaatttcttt
     7621 gaagtagatc acagatacat aattatagca actttgtatt cattatataa agaaaagaaa
     7681 attaaagttg cagttgtaaa caaagctgta aaaaatatgg gtctggatcc aaaaaaacct
     7741 aacccgttag aagtttaaat tagatttatt aatatgttat tttttaattt tgtaataatt
     7801 taaaatgata aaagaaataa agctgcccga aatatcagaa aatgttgaac atggtgatgt
     7861 tataaaaata cttgtaaaag tgggtgataa catcacaaaa gatcagtcaa taattgaact
     7921 tgagactgat aaagctacgt ttgaagtacc ttcgactgaa gaaggtatta taaaagaaat
     7981 attgataaag gaaggtgacg taattgatat tggtgctgta attattaaag ttgaaacaaa
     8041 ttcaaaaaac ggatcttctg aatcagaaaa taatgataat tcaaacaagg aaaatggaaa
     8101 taaagaaaaa gataatgatg atgaaggtaa agctgaagaa aaagtaaaag aatcggagag
     8161 cagtgaaact gatgatgaaa gcaaaaaaat aaaaagttcn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnt gataaagaaa gttctgataa agaaagttct gataaagaaa
     8341 gttctgataa agaaagttct gataaagaag aaatagtcag atccggtgac aatattaatt
     8401 tacttgaaaa aataaagaat aaagatcttg cacctgcatc cccttcagtc agaagacttg
     8461 caagagagct tggagtcgag gtgggagatg taacaggaac gggaatatca ggcaggattt
     8521 ctgaagatga tatcaagctt tatgttaaat ctttaatgaa agaatccgga tcaatttcaa
     8581 taacaagtaa agctttacct gattttagca agtggggtga aatttccaaa gaaccaatga
     8641 gcaaggttag atccattact gcagaatcaa tgacatatgc atggactact ccgatggtta
     8701 ctcagtttga taaagcagat ataacattga tggaagagtt tagaaaaaaa tatgcaaaag
     8761 cagtagaatc aaaaggcggt aatcttacta ttacttctct gcttgtaaaa atatgtactg
     8821 aggctttgaa aaaattccct cagttcaatt caagtattga tgtaaataaa aaagaaataa
     8881 tttacaaaaa atattataat gttggaattg ctgtagatac ggacagagga ttacttgtac
     8941 ctgtcatcag aaataccgac aaaaaaaata ttacagaaat atctgttgag atgaacgatc
     9001 tggcagaaaa agccaggaca aaaaaaatca cacctgacga aatgaacggg gggaatttta
     9061 cgatctcaaa tctgggtggt attggcggta ctgcatttac acctatagtt tattctccgc
     9121 aggttgcaat acttggagtt tcaagagcat ccagggaagc tgtatttatt aatgaaaatt
     9181 ttgaacctcg tatgatgctg ccactgatgc tgacatatga tcacaggata attgatggtg
     9241 ctgatgccgc ccgattttta agatgggttt gtcaggcttt agaaaatcca atgacaattt
     9301 tactttaaaa taaaatttct gctgaaattt attttttcag taaatcacat cagtctattg
     9361 aaaaactcat atttacaact acccttatag ttttatcaat tgaagtagta ttatttattc
     9421 cataatcaga tatctcgttt gaattcttgg cattgatctg tactactccc attcttgacg
     9481 atctcacttc accaatttca ttttctgtac tatttgcaat ctgttctgct ctgacttttg
     9541 catccatagc tgcaagacca gtcatttcta ctttaagatc tgataatttt gtatatagaa
     9601 attcaggagt aggtgaattt atctcgattc cttcgttaat taattccgtc gcttcacgtg
     9661 aaagcttatc aatcttttcc acatcttccg attctactct gaaatcctga ttcaatctat
     9721 agccggtgac ctgattggtt gcatagccct gatcattgct tacatataaa gtagtggtgt
     9781 taattgctga gacagtaatt ttgtccgccg gaaatccttt accaataaga taatttttta
     9841 ctttttcatt tgcttctttc agctttgcat aagaatcctg aagagaaaca gcttcaatag
     9901 aaatagatcc gttccagatc gcataatcag atttgatgtc ttttgacgca gagcctgtga
     9961 cattaattgt cacgtctttt cttgagacat atttccaagt tgaagaaatt ataaatgctc
    10021 caattataaa tcccgctgcc agtacaatag ctgccggaat ataattcctg aagttgttta
    10081 ctgtgttatt attattcata tttattataa attaatttct ttatcagggt taaattttct
    10141 tgatactttt ttttctgatc tctgcttctt aagacgaatc cttttctctt taactgcaac
    10201 tgttggtttt gtatcctttc tctttttttc aggtgttaaa gctttttcaa aaattttaaa
    10261 aaatttttca acagcttctt ctttgttcat atattgtgat cggctggttt gagaagttat
    10321 ttttaaaatt ccgtcagacg ttaatttttt aaaaagcttt tttgaaatta attctctttc
    10381 aatattactt aatacttttg aatttaaaac atcaaaatta agctcaactt ttgtttccac
    10441 tttatttaca ttctgtccgc ctttaccacc gcttcttgaa gttttaaata caagttcatt
    10501 tcttaattct tctttattta attctattga actcactttt tctttggttt ataaatatga
    10561 gtgctctgtc cgaaaaacac ttctgcagat tccataatag tttcagataa agtcggatgc
    10621 ggatgtatag taagtttcag gtcagttgca tttgctccca tctcaattgc taaagcccct
    10681 tctgaaataa gatctcctgc atttggtcct acaataccaa cccctaatat cctctccgtt
    10741 ttattatcaa ttattaattt tgttaatcca tcagtccttc caattgtcaa agctctaccg
    10801 gatgcagccc atggaaattt tgacactgta taatcaagac ctttttcttt tgcttcattt
    10861 tcagtaatgc cgcaccatgc aatttcagga tctgtaaaaa caactgcggg aattgtcttt
    10921 ggctcgaatg ctgatttgtg acctgcaata atttctgctg cgactcttcc ttcatgtgaa
    10981 gctttgtgtg caagcatagg ttcacctgcg acatctccga ttgcaaaaat attattgaca
    11041 gaagttcttg tctgattatc agttatgata aatccgtttt tggaaatatt tattccggca
    11101 ttctcaagtc cgagatctga tgagtttggt tttctacctg cagaaattaa tacactttca
    11161 taaatttttt ctgatacctt attttctttt gactgaagtt taactttaaa gcctgattta
    11221 atttccttaa tatcaattac ctttgtattt aataaaatgg attcaaattt tttgttcact
    11281 ttagtagaca gtacggatac aagatcacga tctgcaccat ttagtaaacc gggtaacatc
    11341 tcagcaatag tcacttttgt tcctaacgca gaataaacag tcccaagctc aagtccaata
    11401 tatccacctc cgatgacaag cattgtttta ggaattttat taagatcaag tgcagtagtt
    11461 gagtctaaga tattttttga agaatctgat aaagcgggta ttctggttgg atatgaacca
    11521 gttgcaatta tagcatattc aaattcaatc ttttcttttt tatttttagt attagttaat
    11581 tctatagaat ttttatcaag gaaagtagct gttccttgta tatactcaat ctttctctgt
    11641 tttgaaagct gacctaaacc ggatgtgagt ttactcacta catcattttt ccatgtcctg
    11701 atctttttaa gatctatttt aggttttgaa aaattcactc cgaaattttt tgattcttct
    11761 gcttcactaa ttatttttgc aagatgaaga agtgcctttg atggaatgca gcccctgtaa
    11821 agacaaactc cgcctggatt tacatcttta tcaatcagca ttgtctcaat cccaagatct
    11881 gcagctaaaa atgctgctgc atatccgccg ggtcctgctc ctattacaat taattttctt
    11941 ttactcaaat tttaagtttt tttaatttaa caggaatata caaaatctta aatgaatttt
    12001 acaatcattt ttaactattt aatttattta aaaaaaaaaa tcttaactta aactattgca
    12061 ttaaattata attaaatatt atatatattg ttgatattta aaaacctaag aaagggaaaa
    12121 ttaatgaaac taataaagta ctttttaata gcactttttg taacaacttc tgttcaggct
    12181 caaaatgtaa aaattacaga ttttgatata cctgtgagta gtgcaaggaa attcttaata
    12241 aacggttttt ataactgggc tcagacagat ccaaatgatt cagctttaaa tttatcaagt
    12301 tcatggcaat tagacggtgc ttatacacaa ttttatactt caccttctta tgcatggcat
    12361 ttaaatttaa ccggttcatt aaaccagtta actagagaag atacaaccag atataattac
    12421 aatctggcta ctgacctaag taagtatttt agtgattcac atggttggtt tggtaatgca
    12481 gaagtcagtt caaattatgc aagaaaaaga gaattcggaa cagataacag acctactatt
    12541 gaaatttttg gaggactggg atacggaaga caagtaaatg caacatcact tgctaaagcc
    12601 gtcagaattg acgaagatct tagaaaaggc ggaattacaa ataaatatat gcctaaatct
    12661 actgttcttg caattgcaca gatcgtcgac agggaatctg aatacagaga taaatataaa
    12721 gctttgtatg aatccaagat catagaagat atttctacag aagttatgaa ttcaggagta
    12781 acaaataaca ctagtatgtc agctttagga tttatgagga tccgtgacgt tttatacggt
    12841 ttaaatcaat ttataaatga tagaacttac ggtggtgaca taagattagg tgtttcatat
    12901 caggtactta caagaaataa agaacttaca ggtgcaccgg ctttcttaag tcttagagga
    12961 agatatggat atccaatcgg attaagtcat cagatctctg cttttgctaa tgcaggaaca
    13021 gcacttgaca gtgcattcgg aaaattgttt accggaaatg cagggtttgc ttattcttat
    13081 aatatgacaa acagagtttc ctttaatgca ggttacacag taacatttaa tcagatattt
    13141 gatcaaacca atattaataa tgtaggtgtt cccttcggaa cagataaatc aggcggagac
    13201 aatacagcaa ctgcaggatt tacattctat attgaaaatt acataactct taacttaacc
    13261 ggtggatatt caacaattta tggaactgag caaagatggt tctctaatgc ttccctcgga
    13321 ttaattgtat tctaaaacag atttaattat ataaacaaaa aagtgcctga atttcaggca
    13381 cttttttttt attctgatca aaaatctgga aactgtttta taaaaaaaat taaaatgtta
    13441 ttttttctgc accgcctgtt aatataacat ttaataattt ctttccatca atttcaaaat
    13501 ccactatcaa tgattcaccg gatctggtaa gcagttttac aggcttaaac tgttccctcg
    13561 tcatgtagta aatcaaagca gatgaaatag aaccggtacc acatgcaaga gtttctcctt
    13621 caacacctct ttcataactt cgaatcttca attcaccttt tttaattatt tctacaaaat
    13681 tcacattcgc accttgaggc atcagatctt tgtgcatacg gatatttctt ccccattcag
    13741 aaatatttat ttcgtcaatt gattttactt ttggtttttc aatgtcatca ataaaaacaa
    13801 ctatgtgcgg agaacctact tccacatatg agcagttaag taaatcccac cactcctgaa
    13861 aatgcacctt tagtttaaaa tttgttttga ccgttaatgg cggtggaaat tttacagcta
    13921 tttcattttc agaaattatt ttacattcat aaatttcaga taccgcttca atatttaatt
    13981 ctttcttatc tgaaatttta ttatcagcaa tatactgaac tgtacatctc aaaccatttc
    14041 cgcataatgc atctcctgtt ccgtcacgat taaaataatt cattttgaaa tctgcttttt
    14101 ctgaattctc aagaaaaatc actccatcca tacctggatg attttccata agaaactcaa
    14161 ccttattttc atattcaatt gtttcatgat ttcgattatc atttattaca aatgaatttc
    14221 cggcacccga atatttttga atgaccatta ataaactcct ctcgtgtttg tttttttagt
    14281 tacttttata tctcttaact gcggtgcttt aactcttact tcaaaagaaa aacctctgta
    14341 tgttccggtc ggataccagt taaaattcat ttcccatgaa ttgagatctc tgtaagcagt
    14401 aatatatgga gtgactatct gcttatttac aagatcataa tttgctgaaa ctgtgaatct
    14461 ccacttttga gtaagattca atgaaagatt tccggtcaga ttggaagtcc tgtttactat
    14521 tgccggatta aattttgact cggaataatt aaatccaaat ccaccgctta gaggaatttg
    14581 aaaatctatt tcatcaagct tatctttacg gaagtcttta tagtcgaccc ttgtagtcag
    14641 agtatcgctt tctgttatgt cttcacttac tccagaagta gtaggttcag gagcaaaact
    14701 aaaaccggtt gataaattta ttgtgaaatt tgtaatgttt gcaagtacgc cgtcagtatt
    14761 gacaagaaac tgatttatcc tcgtgtttgc agtctgatca aatttatata aattaaaaga
    14821 agtacctccc gaaatattca gaaaatttcc gatacttgtc ctgtaggata gcaatatctc
    14881 agaaaaattt aatgagtctg ctacgaaatt ataagcagct ccggcgtcaa tattcagtag
    14941 ttgaaattta ttttcagtcg aatcattcac gcgggttttc atttcaaaaa cattacccag
    15001 actaaaagca attgactgtg attcacctga cggagctcct ccgaatattt ctctttcgta
    15061 aattgaatat tttatttcct ttccgtttgc atctgtatat gactgataat atccccagcc
    15121 cggatcagaa aaatccggct gataattgta tgttatagtc ggagtaattg tatgccgaat
    15181 tcctgtaaca ttaaatattt ttggaaggaa gatcccgaca aactttgtat tcagacttac
    15241 acctgtattg aaatatcgca gcgacttgat ggcgttgttg tcatttgttg tgactgaact
    15301 gtctgcagga ttgaaatttt tactgacata tttattgtac caaatttcat tataattaaa
    15361 aaatggtgta agcgaaatat actggaactt cggagaaagt cctatattta ttgaatttct
    15421 tactcctgat cgtaaatcag tataagtgct gtcatttccg gcagcatcgg ttattgtgcg
    15481 ctttatgctg ttatttaaaa tacttccgtt gtatgaaaaa aatatgtact catataattt
    15541 cttattgaac ggatccgaaa atcccgattc aaaagggaat gtctgcgata ctgaaaaatt
    15601 tatcaaaggc agacgctgag ttacatcgcc ggtttgaaga ttttgatctc tgtaataatt
    15661 catgcttaag gcaaatggtt ttccttccca ggcttttgat aatgtaaagt ttgagataag
    15721 attctgaagt agtaattcag aaagtgcatt agtcgaaaca ttataaaagt ttttactgct
    15781 tgcaatctgt atactggcat caagtctggt agtcggattg aactgctgat tatgaacgac
    15841 atttaatccc catgcatccg aattctgatt tccaatgtcc gtactttctc caagacgtat
    15901 ctttgaatat cctccgtcta ttgttccgga gaaattatat tttgaagcat atctgtatct
    15961 accataaatg tccgttcttc cttttgtaaa ataacttccg gtaaatccaa tatccatata
    16021 atcatttaca gcccagaaat atcctaatct ggaaatatat tttccgtagg tggcatcatc
    16081 accaaaggta ggcataataa gtcctgatgt cctgcctgac ttgtttggaa atactccgaa
    16141 aggtatccac aaaacaggca caccttctat atacagaaat acagactttg caatcacttt
    16201 gtcatttgga ataactttca tttccggtga aaggaaataa tactcaggat cttccctgtc
    16261 agaagaagtt gtataaattc cgttctttat aaacagaaca tccgaagatt ctttctttat
    16321 tttttctcct gaataatatc caatctcagc ttcgctgaat ccttcagaga cacttccctg
    16381 ctgagtctta aaattataaa aaagtttagt accttcatat ttatcactac cctgcgtcat
    16441 gacaggcatc tgcattgctt tctcaggatt tgccgaatca ggtatcccaa acgcttctag
    16501 gatctgtgtc tcctgatcta ctataatttt acccgcttcc agtctgagat ctttataagt
    16561 taaaattccc tgatcataaa ttattacttt tttattttta agatcataga ctattgaatc
    16621 actgccggta taattaatta ttgcatctat atcagacttt gcagtccttg atgaatcaaa
    16681 tgaatttaca tttgcagaat ccagactgcc aattactttt gaatttagac tgtcagtatt
    16741 ttccaaaaat gtatctgatt taattatatc caggctatct gtattttcca atgaatcagc
    16801 cttgtcagta attgtctgcg cattacagat atttactgaa aaaataaaca gcgttataaa
    16861 aaaaatatac ttcatcgaat accattttac ttaaatcaat ttactaaata aatgaaatta
    16921 taaaagagca atgttcaatg ttcaatgtat aatgtataat gttcattgtt cattacattt
    16981 tattatgcag tttaaaaaag aagatttatt ttaaagaaca aaccaaattt aagtttttct
    17041 taaatagaat ttaataagta aaataggtaa tttgataaaa attttatata aacatatgaa
    17101 aatcgatctg atttatgaag gtaaagcaaa aaaaatatat tcgactgata gggaggatgt
    17161 atatttacag gagttcaaag atgatgcaac agcatttaac ggagtgaaaa aagataagat
    17221 ctcacataaa ggcgagatca ataattttat atctacagag atttttaaat atctcagaga
    17281 aaataccata cctgttcatt tcattgaaaa gatatccgaa acggaaatgc tcgtatctaa
    17341 agtagatatt ataaaagtag aagtcgtctg ccgaaatatc gctgccggta gtcttgttaa
    17401 aaagtatggt tttactgaag gtgaagttct tgaaaaacct atcatagaat tttatctgaa
    17461 gtcagatgaa ctcggagacc cgttattttc tgaagagcat atatacgcga tgaagcttgc
    17521 cgataaacat gatatagaca tgatcagaaa aatgacgact tatataaaca cacttctgca
    17581 gaaatttttt ttagaaaaag gaataaagct tgtagatttt aaattagaat ttgggaagga
    17641 taaacatggt aatattatac tcgctgatga aatttctcct gacacctgcc gtctttggga
    17701 ttccgagact gataaaaaac ttgataagga cagattcaga tttgatcttg gcgaagtaga
    17761 agaggcttat caggaaatta agaacaggct gagctgatga aatttacccg ctacggaaaa
    17821 gatgttatac ttaaagtttt tattctgggt ttggtgctga cagtcctttc attatttatt
    17881 gataatatta ttgtaaaatt tttattcacg ggaataacta tttttctttg gctgttcaca
    17941 ctttattttt tcagggatcc ggacaggagt ctgccaaacg gatacaaaga aaatcagctt
    18001 atttctcctg ctgacggaaa gattgtagta attgaagatg tgataaataa agaagataat
    18061 ctttttaaaa aagatgagcc tctgaaacag atcagcattt tcctttcccc tctgaatgtg
    18121 catgtaaaca gaaatcctat taatggaaaa gtgacttact taaaatatat agaaggtgat
    18181 tatctcgtcg catttgatca taagtcatcc gaaagaaatg agagaactga gattggaatt
    18241 gagaatccgg agaacggatg taaagttttc tttaaacaga tcgcaggatt tgtagcgaga
    18301 agaattgttt atgatctaaa agttggatgc acagcaacag ccggcgacag attcggaatg
    18361 ataaagttcg gatcgagagt tgatatttta attaagaggg attctaaaat attagtagag
    18421 atggatcaaa tagtgatcgg cggtgaaaca ataatagcag aattataaat gtttatttca
    18481 agaaaattca taccgagtct gttcacaata ttaaatgctt tctgcggaat gatgtcagta
    18541 atatattccg ctaaaggtga atttgatgaa gcggcattgt ttataattta tgcatcactc
    18601 tttgatgcag tcgatggtct cgccgcaaga cttaccaact ccgccagcaa atttggtgta
    18661 gaactggact cactctcaga cgtcatctca ttcggtctcg caccgtctat tctaatttat
    18721 tatgtctatt ttaatcagct tggagatatt ggaattgtaa tgtcatctct gataatggtc
    18781 ttcgctgcta tcagacttgc aagattcaat gtgcagctta cgggttttga aaaagaaaaa
    18841 ttcaatggtc tgcctgctcc aatgacagcc tttactatat gcacatacat acttttttat
    18901 cacaacaata tcttttcaga aagtgtcagt gaaattttta taatctcttt agcatttttt
    18961 ctgcctgtaa tgatggtaag cagaataaaa tatgactcca ttccaaaacc ttcattacat
    19021 tcacttaaaa aaaatccact ggtattcatt atcatattca ttggtataat tttagttata
    19081 gctactgaag gtgaaggctc tttttctatt tgtctgtttt atattttttc gggcatcatc
    19141 aggaccggct ataaaacttt ttttggaaaa cataaaaagt ctgatgatgt aaatgagtct
    19201 cttgaattca ataaatccaa ataacctttc tactttttct ttacattctc ttttaaaact
    19261 tccagtaatt tttcaatttc atttaaagta ttacttcttc cgaatgaaat ccttatagat
    19321 ccgagtgctg tcctttcatc atgaccaagt tcttttaata cataagaagg tttaagcgta
    19381 cccgctgtac atgctgatcc gcctgagaca gcgatgcctt tgagatcaag ttttattaaa
    19441 agcatctctt catcatactc aagaaatttt ttaccaaatg agatatttac tatgttttgg
    19501 agacaattct ctgtatcaga attatagata attcctttat caaaattttc atttaaacct
    19561 gaggtaagaa tatttttcag ttcagtataa tgtaaaacat cttcagtcat tcttcttttt
    19621 aaaatttcaa tagctttttt aaatcctgcg atcgcagcaa tattctccgt accgccccgt
    19681 agatcccttt cctgtttacc gccgtgtatg aatttatcga caatggtttt atcctttatg
    19741 tataataccg caatgccttt gggtccgtag atcttgtgtg ctgaaatcgt cgcaaagttt
    19801 atatttatat ctttaaaatt tagatcagtt tttcccagac tttgaacagt atccgaatgt
    19861 acgtagatat ctttttctaa aattgattca gttaaactct taatgtctgt gattgcacct
    19921 gtctcattat ttgaatgcat tatggaaatc agaaatgttt cgggtctgat actttctaaa
    19981 acctcttcag taccgattct tccaaatcta tccggcttaa tatatgtgac atcaaaatta
    20041 aatctctctt ccagatattt taatgtgtcg agcacggctg aatgttcgac tgttgaggaa
    20101 ataatatgat ttttttcagt gccgatatgt ctgaatgctg ttccttttat tgcatggtta
    20161 tttgattcgg tgccgctgtt tgtgaatacg atctccttcg agcttacacc tgtaaattcc
    20221 gccaacacgt ctcttgcatc ctcgagcatg acctttgcag tctttccgta tgaatgtata
    20281 gaagatgcat tgccaaaata attcttcaaa taaggaagca ttgcatcaaa aacttcttca
    20341 tctatttttg tagtagctgc gttatcgaga tatatctttt ccaaatttta atttaagtta
    20401 atatattaaa attctgcctt cttcaactct gaatacaaaa cttttgcctc gaaaactcaa
    20461 agagtctaag ttaaaaaaag ttaaaaaaaa tttaaaaaaa tcatccttga gccttaaagg
    20521 ctttgagaca agctaaatct ttagacaaat gtgatattat aatttgattg ttacttttat
    20581 ttttaaaatt cattatttta tcccgtgcct ataaataaag aatactttta tatctccaac
    20641 ttactttcgg tattaaggat cgtacttctt ataccactga gctggctgat gctgacagaa
    20701 tttgacacta aaaataattt aataattatt ttagttctat gtatgtattt atctgattta
    20761 cttgatggat atcttgcacg aaagctgaat gaagtttcgg aatttgggaa aattattgat
    20821 ccgcttgcag acaaaatttg tgtgatcgca atagcggtta tattagttta tttagacaga
    20881 cttcctcttt ggttcgtatc tgttgttgtg ttaagagata ttacgatttt aattttcggt
    20941 gtatatctca aaaggaaaaa ggatataact ttaatgtcaa actatccggg taaaatagct
    21001 gtcttctgta tcggattggt attactgttt tcaattattg acagtgaatc tttaaaaatg
    21061 atttcaacta ttatgtattt tgtttcaata ttgatgatcg cttactcttc acatttatat
    21121 ctgaaaagat atttagaaac tgtcaggaaa cctgtttaat attttattat gaaagaaaaa
    21181 attaaaacat acaccagaaa agatattgaa cacagactcg gcgataaact tgcagggaaa
    21241 cagcttacag aaaaattatt agttgactca ttatttgaat gtcttcgtga agtcttactt
    21301 gaagctgatc ctgtctcaag gcttgagatc agagactttg gtgtatttga aattaaaaag
    21361 accaaaccca aacctaaggc aagaaatatg cagaccggtg aatttatttt tgtgcccggc
    21421 agacggaaga tacatttcaa accgggcaag atcatcaggg attttttaaa agaagattat
    21481 gatgaaatta ttataaaggg tacaaacgat aaagataatt aataaaacta aataagttgt
    21541 tgaatacaaa atacatgggc attgattacg gtgaaaagcg ggtcggtatt tctgtcagtg
    21601 atgaaagtaa gaaattttct tttaacaggg attatatttt taatgataag gattttttta
    21661 aaaatctaac aaaattgatc atcgctgaga atgtatcaaa tatagtgatt ggttatcctg
    21721 taaagctgaa ttctgacaga actgaacaaa ctgacaatgt cgataaattt gctgaagatc
    21781 tgagaaaata tttagagtca aataatgtaa aagctgagct ggcttttttt gatgaaagac
    21841 ttaccagcaa gatcgcagcg gaaagtcttc tcagatcagg aataaaaaaa atgaagagaa
    21901 gagaaaaagg tttggtggac ggaatatcag cgcagcttat actacaggac tatcttgata
    21961 aaatgaatta ttccggcagg tgataagtta gtaagaatgc ttcggcattc aaaaaatttt
    22021 aatattaaga agatgtatct aatagaatta catgaaatga ttgaaggagg agagaatgaa
    22081 aatgtggagt tcaaaagaaa attttcttca ccggaaaaaa ttgcaaaaga gatgatcgca
    22141 tttgcgaatt caaaaggtgg aaaaatgatc ttcggagtgg atgatgacaa aagcattgtc
    22201 ggagttgaaa gcgagaaagg tgaactggaa ctgatctcaa ctgctgcgag gcattactgc
    22261 gaacctgaaa tagaattcaa aaccgatatc atgcttctgc gaaacaaaga cctcgtagtc
    22321 gtgaccattg aagaaagcaa aatcaaacct cacagactta tctcggatga tgatgaaaaa
    22381 tctgaaaaag tttatataag atttaaggac aggagcatgc tcgcttcaaa agaaactgtg
    22441 aagattctga gaaattcaaa cgcagatgca catccggtca atatcactgt cggtgatatt
    22501 gaaaagacat tgtttgattt tttaaataag aatgaaaaga tcactgtgaa aggatttaaa
    22561 aaacttgtaa atatttctga aagaagagca agcaggactc ttgtcaatct tgtaaaagca
    22621 aaagtcatca gacataattt tatggggaag gaagattatt atactttgat atgaaattgc
    22681 aaatgtcaaa ttacaaatga ctaataaaga gtcagaaaat aaattaagaa ttataaatca
    22741 ggaattaaga attgttaatt acgtactacg gtagagaagc agaatgcggg aagttattct
    22801 gtggagtttg gcgggagcgg ttttgcgagc gggatttatt tttattcgtt gtcagtaaac
    22861 ggaaatgtaa ttgatacata acgtatggtg cttttgaaat aattttgata taatccatcc
    22921 aaaatctatc gaaagatttt taatttattg tttttttatt cgaattagaa aacctatatt
    22981 tcaaccggcg attcttaaat tcagccgcaa aaataaaata tttttcaaaa ttaaatcagg
    23041 aggttaatat catgccgcca agtcagggag aatggaatat tctaaaagta acaatcaata
    23101 atgaagaagt tgatcttgat tcacaggtca ctgcaattca tgctcactta attcctgttc
    23161 caacaggatc agaaagtcat cctaaaggaa aagtcttata ctttcatgca agatatccaa
    23221 gtaacaatgt aaactttgaa ggagaaccaa cgccaaattt tgtttcattt ctatttgatc
    23281 ctgaaacaaa tgagatgact caattcacaa tcccaagatt tcctgatgat attggagtag
    23341 atgtagatcc ttcaaaacta ttttgcagcg gtcatacatt tcttgctgac ggaaaacttc
    23401 ttgtagcggg aggagaaaga aatcaaccgc cctggggttc taacggaaca aaatattcat
    23461 ttttatttga tccttctgtt ttaggagata ccgctccttt tccctggaaa tttacgggtg
    23521 gatctacagt tataacaatt actaaaatga gccgcggcag atggtatccg cagcttacca
    23581 aacttcatga cgggacagta ttagcaatgt ccggatatcc ggaaactcct ccgcctccac
    23641 aagatccgcc tgaaattact gtaattatac ccgaacgata taaccctgat actcaggaat
    23701 ggaccccata cacacatatg gaggatggtc ttcgaattcc tttatataat ggagcttatg
    23761 taattccttt cggtgcctgg cagggtgaaa tattttatga catggcagca ttcggaccgc
    23821 aattagaaga atggaatgga gctcataggt tcaatccatt tggcacaaca gatttatgga
    23881 atcctgtcgg ttcacaatct aaatctgtca ggctacacgg atgctctgta ttgcttccga
    23941 taaaatctac agataccgat atcaggatag taaacatggg cggatttgat gataattcta
    24001 aaagttgcga attaattgaa ataggcagca atacgagcac tgattgggta ccgattacag
    24061 acttagaaca tcatagacat gattgtccaa atgcattact gcttgctgac ggaagtttaa
    24121 taattatcgg aggtaatgaa acattaattc ctgaaatgct tgactatagt gatccggatc
    24181 cttctaactg ggagtggtct tctttacctg ctatggaagt ccctcggaaa tatcattcaa
    24241 ctgctttact tttaccagac ggtacggttt ggtgcggcgg aagcagaata tatactgatc
    24301 cgctgaaatt cgaattcgaa aatgatatgg aaaggagaat tgaaatttat cgccccggat
    24361 atttacttga aggtccggga cctgtgatta catcagctcc tttagttata aactatgatg
    24421 atgtttttga agtaggttat aacattacag cagagccatc acctgagatt gattctatcg
    24481 ttttaattag cttaccttca gttactcact gctatgacag taatcagaga tatttaattt
    24541 tagactttgg atcttcggtt aattttggag ctttaaatgt tacagctcca attgacgaat
    24601 atttagcacc tccgggttat tatatgctat ttcttttaca gaataagaca caaagtatct
    24661 ccggagaagt gagaattcct tccattgcta aaattgtaaa actttttaga ccttctccat
    24721 gaagaatccg aaaattttac tttttgtttt attattatct ttgaaatcct taacaagttt
    24781 ctctcaggtc tctattggat gggtagcgag gtataataat caaggtcttg gatatgatg
//
LOCUS       DAORVQ010000026        23302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_800_length_23302_cov_7.092270, whole genome shotgun sequence.
ACCESSION   DAORVQ010000026 DAORVQ010000000
VERSION     DAORVQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_23302_cov_7.092270"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1645)
                     /locus_tag="PLX80_03865"
     CDS             complement(281..1645)
                     /locus_tag="PLX80_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY19966.1"
                     /translation="MDFLDKLVIPATTQHLSLLKIILVMSLLIFIPFFGMLMGGTAFS
                     VIFNAYGRKMKNKLFIRFAKDVIDKLAINRFVGVGLGIIPLLAITFCYAQLLFEVKVI
                     AVSLLFVATLFLAIAVNFIYSYQNTFQIESLIETFKGIVGIDKHELDKQLPEDVTNYE
                     FDIMNTNSNSGLYGFVLLLVTALFFAGGITISQFSERYQDFQNIAQIFLSGATFVNFI
                     FIIVLSAAITGSAILFFFFSWQGGMHDMDKEYSNFVKKIAGNMAFIGAALTPVVLFFS
                     FYLTPNSALSGSVFIYTGLAFVSLLLLCNFLYAVIKNSDMKYIGAAFYLMILSCGFII
                     MKNQSAFGTANFKHLEAINLKAEELHKEKKSKTINTEGVNGEEIYTKICSACHSFDTK
                     LVGPPYAQTVPAYNGDVKKLANYILDPTKKNPDYPPMPNPGLKPKEAAAVAQYLIDKV
                     AGKK"
     gene            complement(1669..2514)
                     /locus_tag="PLX80_03870"
     CDS             complement(1669..2514)
                     /locus_tag="PLX80_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HQY19967.1"
                     /translation="MTKIRITILVALTTSLGYILALTDLSAFSLYPVIGIFLLACGAA
                     AMNHYQERGTDILMDRTKGRPIPSGNVSASFVITVAIVFLLAGSAVLFFKSSLLTLYI
                     GLFTFLWYNVIYTPLKKVMALAIIPGSLVGALPPMAGWAAAGGSLTDDKILLIGAYFF
                     IWQIPHFWLLLLVYGKDYDKGGFPTLTNMLSQRQLIKITFTWILLTIAVALSINFFGI
                     LNYYISNAMLMILCLWMFVRSVKFLDMTEDKKSFKGMFISINIFTLLIITILSVDKLV
                     KLFLE"
     gene            complement(2605..3417)
                     /gene="coxB"
                     /locus_tag="PLX80_03875"
     CDS             complement(2605..3417)
                     /gene="coxB"
                     /locus_tag="PLX80_03875"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQY19968.1"
                     /translation="MRPSIINEVDTTFWIITGISIILLLIITVAMLYFIFKYSRKRNP
                     VPSNIEGNTTIEIIWTVVPIIIVLYLFFISWNGFINMRDVPKDAMVVKVTGQMWKWTF
                     EYDNGKKSDTVNLPHNKNIKFELSSNDVLHSFYLPHFRVKEDVVPGSPNYIWIRTDDL
                     GTYYAACAEYCGLNHSYMYAPVNVISEDQFEIWKNILPVETKMDSTAAIKDSLSSSGT
                     TGNSDSTGTLNDSTKKVNGTDTAKTKVKSDTSKKETPASDLKKTEEKDTTKK"
     gene            complement(3501..3845)
                     /locus_tag="PLX80_03880"
     CDS             complement(3501..3845)
                     /locus_tag="PLX80_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQY19969.1"
                     /translation="MSNHNSDHSSHSEHSENDDGHIAVAHHPSYGINVLVWIGLLSLT
                     SITVAVAGIDLGSLALTVALIIATVKTMFVINFFMHLKFDNKVFKIFVGVCMLIFIIV
                     LILTFFDLTFRY"
     gene            complement(3870..4508)
                     /locus_tag="PLX80_03885"
     CDS             complement(3870..4508)
                     /locus_tag="PLX80_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HQY19970.1"
                     /translation="MSYNSDTADQEIHEVHIHRDDFGSKMGMWLFLFTEVLLFGGLFI
                     LYASYRFIYADAFVVAAGELDVFMGAINTVILLTSSLTVVLAIVALQKSNKKLSLFFL
                     WVTVFCGLIFLVNKYFEWGAKFHHGIYPKGPALQDMSEGEVMYFGLYYVMTGLHGLHI
                     VVGLAFIGFVMWQIKKDVITATNFQKLENSGLYWHLVDIIWIFLFPLFYLIH"
     gene            complement(4505..6085)
                     /locus_tag="PLX80_03890"
     CDS             complement(4505..6085)
                     /locus_tag="PLX80_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY19971.1"
                     /translation="MAWLLTTDHKRIGLMYLSAMSVFFLAAMTIGVLMRLEMLTPGET
                     IMSAQTYNSFFTLHGVIMIFLFIIPGVPAVFGNFMLPIQLGAKDVQFPKLNLMSWYLY
                     MIGGGLAIFSLFIGSGIADTGWTFYAPYSIRTTTNVSISVFAAFILGFSSILTGLNFL
                     TTIHRLRCPGMTWFRMPLFVWSVYATSWIQVLATPIIGITLLLVILERAFGIGVFDPA
                     LGGDPLLYQHLFWIYSHPAVYIMILPAMGVVSEIIPTFAKKPIFGYVPIAISSLAIAL
                     VGYLVWGHHMFSSGISDTSRVIFSLLTFFVALPSGIKVFNWVATLYKGSIEMTAPMLF
                     TMLFIFIFSIGGLTGLTLGALNTDLHLTDTYYVVAHFHYVMFGGMGLIFFASIHYWFP
                     KMFGKMYNEKVAKVTAYWIAIGFNTLYFPMFIMGYMGMPRRYYDYLPEYQIYHIISTV
                     GSWILVSGILVMAFNLINGIRKGKKCTEDNPWGGTTLEWTVPSPPPLENFDEIPTVTA
                     GPYDHTHIHKQDRQEVSN"
     gene            complement(6174..6977)
                     /locus_tag="PLX80_03895"
     CDS             complement(6174..6977)
                     /locus_tag="PLX80_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY19972.1"
                     /translation="MKKLIISFLFICILFPVINGNLFSQESEGEERKLPDIGIREKLG
                     DFIPDDVILNDENGNPVNLKSLINKPTVFSFVYFRCPGICTPLLNGLTKVIDQADIEP
                     GIDYDIVTISFDQTEDFTLAAGKKETYLSTLDKKISPGSWRFLSGDSLNIHKITDAVG
                     FKFARQGADFMHGASLIMISPEGKVIRYLYGTDYLPFDLKMAVTEAAEGKSVPTIAKI
                     VKMCFSYDPAGRKYVLNVTRIAGGGILLLLGIFVGYLTLKKKKTNLQNT"
     gene            complement(6993..7946)
                     /locus_tag="PLX80_03900"
     CDS             complement(6993..7946)
                     /locus_tag="PLX80_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQY19973.1"
                     /translation="MSHNNNSKGKFNIYDIFRDMEKFYGVLYMLLLVFLVMLGAKYVS
                     TLDYNKLFTAPGLLSADSNMRTPLPVNKGTMTPPVDVIFFSTPSPDLVSQGKDIYAAS
                     CQSCHGETGEGNGPAGTSLNPPPRNFKDPAKQTWKNGQTVSQMYVTLQEGIPNTGMAS
                     FSTIPPKDRFAVIQYIRTFNPLYPQDTPEELTKLDEKYSLATGVKAANQIPVELAMDL
                     MVLEQDTLKNDLEKILIKMELDKSDSAVVILDKIISDKKTAMLSLANDMKWATSNEEF
                     MNFLKTDPLDKGFKGDVYQLSAADSETVWKYLRTLFSVNKV"
     gene            complement(7972..9144)
                     /locus_tag="PLX80_03905"
     CDS             complement(7972..9144)
                     /locus_tag="PLX80_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HQY19974.1"
                     /translation="MDYTKKPLPPIVAKLGTGLLGVGLVLVVLSFVTDTHRAFFDYLW
                     MYMFLVSIGVGSLALVALEYLVGATWSTPFRRISEFLAALIPLLVILVIPLIFGMHDL
                     FHWTHEETLLNDPLLQAKSAYLNVPFFLIRTAIILLIWVVFYFLFIRNSLKQDDTRDP
                     LLTKQSIKFSAAFAILFMFTITFIAIDWMMSLEYHWFSTMFGVYYFAGTMVAAFAAGT
                     FCSVQLNENGYLVSNIKDNNYYSFATLLFGFNVFWAYIGFSQYMLIWYADIPEETFWI
                     MMRMKGSWSYFSIGLIFVHFVIPFLILLPRSSKVKPKLLTIISIWLLFAHAYDLYWII
                     MPSYFTEGFVFGWSELGFLMFAFGLIITVFRMRAAKSNLVPVGDPKLEAGLNFHLN"
     gene            complement(9187..10395)
                     /locus_tag="PLX80_03910"
     CDS             complement(9187..10395)
                     /locus_tag="PLX80_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HQY19975.1"
                     /translation="MLKNILNKIKEFDTAEDKEVRSSKLYGKAALYNTPNEITEAAAV
                     VAGKGYKKFDIYTPYPLHGMDTAMGLPPPKIGYVSFIMGITGTILALVMMIWMSSIDY
                     PNIIGGKPFFGLPGAIPITFESTVLLCGIATVLGLLILFSKMPKLNSPLNDTDFMKHV
                     TSDKFGLVIETGDEMYIEDEVLNLFSKTNAYEVQDIYFRESNLTKKTPLFDKKFTMAI
                     IITGLMTAGVAYVTLNYVLYETVPFNWMWQQGRIDAQSKSDFFPNQSGMRPPVEGTVA
                     RGFVPYEYQGMPDSVVKLLSNPVPVTVKSLERGQDRFNIYCSPCHGYTGDGDGRLRDQ
                     FPKPPSLHNEKVKNWPDGNIFHVMTNGQNVMPSYSEQVSKEDRWAIVNYIRALQRAKD
                     ASEEDLEKAK"
     gene            complement(10457..11791)
                     /gene="nrfD"
                     /locus_tag="PLX80_03915"
     CDS             complement(10457..11791)
                     /gene="nrfD"
                     /locus_tag="PLX80_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQY19976.1"
                     /translation="MDSVIALPTETKPTKGWWIAFLISLTALTIGAVSLGLTIYYGIG
                     MWGNNQPVGWAFGIINFVFWIGIGHAGTLISAILYLFRQKWRTGIARFAEAMTIFAVM
                     TAGIFPIVHTGRPWLAGYLIPLPNMNGVWVNFYSPLLWDVFAVSTYFTISFLFWGIGL
                     IPDLATLRDKTTSKVKKTIYSVMSLGWTNSNRNWSNYERAYLILAGISTPLVLSVHSI
                     VSFDFAVSVIPGWHTTIFPPYFVAGAIFSGFGMVSTILIIVRKIFNLKHIITLNHLET
                     MNKILLATGMMVGYAYGMEFFIAWYSGNTFEQFIFINRAFGPYAWAYWIMVSCNVIFP
                     QLFWFKKIRRSIPITMVLVLLVNVGMWFERFVIIVTSLHRDFLPSSWAMFTPTLTDLG
                     LLLGSFGFFFTFLLLFLKTLPLVSISEVKAVMPGAQPTVHPHPVKEQLKITN"
     gene            complement(11952..14777)
                     /locus_tag="PLX80_03920"
     CDS             complement(11952..14777)
                     /locus_tag="PLX80_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19977.1"
                     /translation="MIVLSPNSYGGLAFDLTDTDSGNRGAVAFGKYKIEFRKFSDQSL
                     IDTCTIEFDAGYPNFGNADCLIYYDRYDDGKISFHWGGANAEEKYIDDPLVDKKIESW
                     IRWGIGVMNKNYGNFSYDNYGSNPYIIFPQDSRYDCNLTNIPNQNQVFTDPRSAVLTL
                     NLTIDKNISTRDTLIDEPTNIVITKKAALKINSNKTFDMITPVNSYNNLIVEDTAYLI
                     LYNGAKIIVDSTNKLTLKNKSNLTLLSNSEIRIKNGAGFCNEGGKVRGPGMIIIEKGI
                     HEFCSYVNDVVIKDSTQIILEDSAVVIMPNSYNFHLRGNTTSLIMKPGSKMMFGENSG
                     IVCDSGAKVIANNAVFTSADSTKKWNGISLKHRSQDTIKNCVIKNADFGINIMNKNDD
                     EGTEVPYSTEISGCSFINQTSHVLNNAIYAVGSSIILIKDNSVSSNILTKGFDHGIYA
                     ELCDGGYFNIIGNTISNSGNGMTILNSSPFVSKNTVNGNQYSESGIFISDSYGKFEYN
                     IINDFYYTYYSLLSSPDLLKNVFNSSYYDNICLSYSSVPNMHPVTSGGSKYWISGDNH
                     FTGSPSNAGIFFEDESYPNMAFGYNRFTLTNNDYYINGTNPTGSDTFDVRENYWGTLS
                     PDLNKINVENSDGLLFNPYDDNSSGQRSVNNYTLYDIGFGLYDTVFFQESDDPGLAQE
                     LYLEAYQKEHSGDYPEAIEIYKEIISDYSDNIGSSFVVSSLSRLFNCYEKKTSTVSEY
                     GALQNYYSNIFNDTSYSESQRNLSEDFIIKTKVKQNNIEEAISDYNTIYLNNINTSKG
                     MHALINKEILSAGEGDNFIGGSGIENLEYKQTRINGILSALINKRFNILSKINTQPES
                     FELSQNYPNPFNPVTKITFNIPVSGNISIKVYDITGKEIKTLVNEFRNAGTYDTEFNG
                     ADLSSGVYYYKMETSNFSEIKKMILIK"
     gene            complement(15054..16493)
                     /locus_tag="PLX80_03925"
     CDS             complement(15054..16493)
                     /locus_tag="PLX80_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19978.1"
                     /translation="MKKIFGILILFCLAAGWGQVKSQSISWERLYDNQNLNDNGVDIC
                     EADSGNFYVVGYSTQSGPNGVLIYIMKIKPNGDTIWEKTLQNHSGKQPFTCASTGDGG
                     CIITGSFSNIFFIKVNNRGDIIWEKNFNEAGVILYEINKTMDGGYIACGRILQSTRNG
                     YLIKIDSEGNIVWKKILSGKFSIEFYSVTEENNYYVAAGYKSDSEISPIDGYLIKLNS
                     SGEIIREREFYFNGVFTSFKKIIFHMNNYIIAGSTSDSSNSLSQTILIKTDTSGKIVF
                     SNIINYYSNEYLQDFKISNNNKFVFTSDVDSTTGRNARIYLTDSFGNIIKQRIIYLSD
                     YTLLRSIQTDRNSRDIIFLGTSKNDVTVNRDIFIVRTDSLLFSQPLSINNNSISFDIN
                     GYVISQNYPNPFNSSTQIKFILAKPSTLRINVNDILGKIVYEKKYSNIVTGSNEFLFN
                     SDKVGSGYYFCNFFINGILMKTILMILVK"
     gene            complement(16637..20014)
                     /locus_tag="PLX80_03930"
     CDS             complement(16637..20014)
                     /locus_tag="PLX80_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HQY19979.1"
                     /translation="MNKPDNKVFDISERIIENSPVETTAELLIPPDTNKAEEPKDLNY
                     WRSFRELYKDPSFKKEKSLEFLPGELQKPDMSAFSKFTRRKFLALLSASAAVAASGCS
                     NYRDRGEIIPYNSKPESITIGNPTYYASTCTGCSSACGILIKTLEGRPIKVDGNPDHP
                     ISKGKICAIGQASVMSLYDPERLKNPVERINRVSPNDIEWKNADEKIIAELKSAASSG
                     KEISIVTHTINSPSQKKLFDEFVKIYPTAKIYSYELFDESEKKNAWEKCYGNKDLPLI
                     NWDKANIVLSLESDFLGSEGNKVETARLFTQGRDAIDGKTFNRLYTVEGNASITGLNS
                     DYRMILKTDAIEEFVMCLLNELTGKERVSNFADDAGITSKLQKYDMKSFAAKNNLSEA
                     VISHLVSDLKKNQGSSIVTAGSMLPESTQIAVNLLNEVIGGSALYRKDESNVTVMPLS
                     TREEMENLVSDLNSGKTAVVIHFDTNPVYHLPTDLGYTEAVKKAKTIITLGESVNESS
                     EIGSFVLPVNSMFESWGDYQTRNTFYSLQQPVIAPLYNTRQKEDVLMTWISGTGDSYN
                     IDNYHKFVMDNWKTGILSSAGTDSDFTKAWYAGLNDGIVMASGKPKLTTAAVTSDSTK
                     TTNSATADSTKTATTLTADSTKTNNTAPILTNTSTAKSAFNPASFISNTNQMTSGEGF
                     SLLLLRNGSLGDGRYANNGWLQELPKAVSRIVWDNYAAISVKSAEALGVDSNDNINIT
                     TSAGKMEIPVFVQPGMADGVIAIELGYGRKVCGTVGEDAGFNANVMMSKSPKLSKWFY
                     NDAKIEKAGGTYEIVSVQDSYPIDEPLYKDIQYRREIIQEGTYLQYKENPNFLSDRHT
                     KYSEEETAKLKVGSINEQYKHTGTKWGMVIDLNKCIGCNECVAACNVENNIPVVGKEE
                     SNNHRAMHWIRIDRYYSGTPEVPKASFQPMLCQHCDFAPCENVCPVAATTHSTDGING
                     MAYNRCVGTRYCSNNCPYKVRRFNYFNFRDNFKNAIQEEESFTLMQNPEVTVRSRGVM
                     EKCTFCLQRIMEEKQMAIQENRQVKGSNVKTACQDACNTNAISFGDQNDKESELYKLN
                     QSKLAYHVLEEIKVKPNITYLAKLRNVYEMETESAGH"
     gene            complement(20039..20302)
                     /locus_tag="PLX80_03935"
     CDS             complement(20039..20302)
                     /locus_tag="PLX80_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19980.1"
                     /translation="MKKNLPVSNSNDNITASGNSPEKINRKKFFRFTGLAAAGLAAVS
                     VIPFGFFKSGSDHKSSKPVSKIKIVSNPNAVKRNSKQSNNRNS"
     gene            complement(20316..20903)
                     /locus_tag="PLX80_03940"
     CDS             complement(20316..20903)
                     /locus_tag="PLX80_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQY19981.1"
                     /translation="MKNFLIDYNLKVRLPILIFVVLSVFAITYYVSYAERVGVGYQPI
                     QPINYSHKLHAGDMKIDCRYCHSDVENSRKANVPSAKVCMNCHTIVRKDRPEIIKLTD
                     YYTRGVPIEWKRVHLLPDFAYFNHSVHVNKGIDCSNCHGDVAGMDVVEQVKDFSMRAC
                     LDCHRNPDKTIKNIMPGKDKSKINTGPTNCSSCHR"
     gene            complement(21124..21573)
                     /locus_tag="PLX80_03945"
     CDS             complement(21124..21573)
                     /locus_tag="PLX80_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQY19982.1"
                     /translation="MKKINLTILALVFSAFIYAGCGDSDNKTESENSDTKKSEETTST
                     SKDENKTAGEEPHKVKGELLAVDVPTIQCGTCTKNIKNALNKVDGVIAVNVSVDKKKC
                     MVDYDASKTDLTVIEGAIVSAGYQANDKPADKTAYDNLNECCKIGGH"
     gene            complement(21911..22441)
                     /locus_tag="PLX80_03950"
     CDS             complement(21911..22441)
                     /locus_tag="PLX80_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19983.1"
                     /translation="MNIKIVLVILISILFLNAAAYSQVKNCDISYFDSTKVRKNLRKI
                     TFEKVISDSLAITDLGNVQKIYLEDIINVGYMSESNPASGLLTGAAIGGGISLGLGIL
                     LSQASGGHPDISLNFGEVVGATLIGGLIGGIIGGAIGGANVKREYKTVDLTKLSLNES
                     YEVINNILETYGEKKK"
     gene            22615..23175
                     /locus_tag="PLX80_03955"
     CDS             22615..23175
                     /locus_tag="PLX80_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19984.1"
                     /translation="MFSVISRLVNGVKGTITSSLKGVSDIAGTVVDVVKKITVNTIKD
                     TADIIKEGIKLPASIVQGALQGVAEIGASVIGAVKGVVNGTVQGVMESGGSQDDAVRG
                     AVGQAMHSAKELGVDIGETALESVKGSVEAVSSVGGNTVEATKSAVMAAMEAAKEIGA
                     EAVDSVKNTLTSGVDGAKQIIDSIKS"
BASE COUNT         7063 a         4621 c         3886 g         7732 t
ORIGIN      
        1 atacgacaat tttcttttta ttcaaaacag atttatagtt aatagttaat agttagtagt
       61 tagtagttag tagtttttat tttagcttac taaattttta tcctactaac attttatcat
      121 tttaacatat cgaaaataat tgtttctata ttagcatcaa ttattttgtc ttaaaatatt
      181 tattgcattt gcattttgac tatgtgtcta tgtgtctatg tggataaaca aaaaaggcga
      241 agtcagactc cgccttttaa aaactttata cattaatatg ttacttcttt ccggcgactt
      301 tatcaataag atattgtgca acagctgcag cttctttcgg cttaagaccc gggttaggca
      361 taggtggata atccggattt ttctttgtcg gatctaaaat ataatttgca agttttttta
      421 catcaccatt ataagccgga actgtctgag cataaggagg tccgacgagc ttggtatcaa
      481 aactatgaca agccgaacag atctttgtat atatctcttc accgtttaca ccttctgtat
      541 ttattgtttt agatttcttt tctttgtgaa gctcttcagc ttttagatta attgcttcga
      601 gatgtttgaa gtttgctgtt ccaaatgcag actgattttt cataattatg aatccgcaag
      661 aaagtatcat cagataaaat gccgcaccta tatacttcat gtcggaattt tttattaccg
      721 catagagaaa attacataaa agtagaagtg aaacgaaagc aagtcctgta taaataaata
      781 ccgatcctga caatgctgaa tttggtgtca gataaaaact aaagaataat accaccggtg
      841 taagagcagc accgataaat gccatgttcc ctgctatctt ctttacaaaa ttcgaatatt
      901 ccttatccat atcatgcata cctccctgcc agctaaagaa aaagaacagg atcgcacttc
      961 ctgtgatagc cgccgaaagt acaatgatga aaataaaatt tacaaaggtt gcacctgata
     1021 aaaatatctg tgcaatgttt tgaaaatcct gatatctctc tgagaattga gatatagtta
     1081 taccgcctgc aaaaaataaa gcggttacaa gtaataatac aaacccatac aaacccgaat
     1141 tagaattagt attcattata tcaaattcat aattcgtaac atcttcagga agctgtttat
     1201 caagctcatg tttgtcaatg ccaacaatgc ctttaaatgt ctcgatcagc gactcgatct
     1261 gaaatgtatt ctgatagctg taaatgaaat tcactgctat cgccagaaaa agcgtggcga
     1321 caaaaagtag actgactgca attactttta cttcaaataa taactgtgca taacaaaatg
     1381 ttattgcaag caatggaatt attccaagcc ctactccgac aaatctgttt atcgcaagtt
     1441 tgtcgatcac gtcttttgca aatctgatga acagtttatt cttcatcttc cttccgtaag
     1501 cattgaatat aacggagaag gcagttccgc ccatcagcat tccaaagaat ggaataaaaa
     1561 taagcagcga cattacaaga atgatcttca gcagtgataa atgctgtgtc gtagccggta
     1621 taaccaattt atctaaaaaa tccattatta atatttatat taaatttctt attctaaaaa
     1681 taattttaca agtttgtcaa cggacaaaat tgttatgatc agtaaagtga aaatatttat
     1741 gcttatgaac attcccttaa aactcttctt gtcctctgtc atatcaagga atttaacaga
     1801 tcttacaaac atccagagac ataagatcat tagcatagca tttgaaatat agtaattcag
     1861 tattccaaaa aaattgatgg acaaagcaac agcaattgtc agcagtatcc aggtgaaagt
     1921 aatctttata agttgtctct gtgacagcat attagtcaga gtcgggaatc cgcctttgtc
     1981 ataatctttt ccatacacaa gcagcagcag ccagaagtga gggatctgcc aaataaaaaa
     2041 gtaagcaccg atcagaagga tcttatcatc agtcagtgaa ccacccgctg cagcccatcc
     2101 tgccatcggc ggtaaagcgc ctacaagcga gccggggatt attgccagtg ccatgacttt
     2161 tttcaatgga gtgtatatca cattatacca aagaaaagta aaaagtccga tataaagagt
     2221 taataaactt gatttaaaaa agagtactgc tgagcccgcc agtagaaata caattgcaac
     2281 tgtgatgaca aatgaagctg agacatttcc agatggaatt ggcctgcctt ttgttctgtc
     2341 cattagaata tcggtacctc tttcctgata gtggttcatt gcagctgctc cacatgccaa
     2401 taaaaatatt cctatcactg gataaagtga gaatgcactc agatcggtca gagcaagtat
     2461 atatccgagc gaagtggtca aagctacaag tatcgtaatc ctgatcttcg tcaattccaa
     2521 tatggctttt gtacggaatg caagtcccga acttacccgt atctcttttt tatatgtatc
     2581 tgctgtttga atttttctta atatttattt ttttgttgta tctttttctt cagttttctt
     2641 cagatcagaa gcaggtgttt cttttttgga tgtatctgat tttactttgg tctttgctgt
     2701 atctgtgccg tttacttttt tggttgaatc atttaaagta ccggttgaat ctgaattgcc
     2761 tgttgtaccc gatgaactta aagaatcttt tattgccgct gtagaatcca ttttggtttc
     2821 taccggaaga atatttttcc atatctcaaa ctgatcttcg gaaatcacat taactggagc
     2881 atacatataa gaatgattta acccgcagta ttccgcgcag gcagcatagt aagttccaag
     2941 gtcatcagtt ctgatccaga tataatttgg tgatccgggt acgacatctt cttttactct
     3001 gaagtgtggg agataaaaac tgtgaagcac atcatttgaa gaaagctcaa acttaatatt
     3061 cttattatgc ggaagattaa ccgtatcgga tttctttcca ttatcatact caaaagtcca
     3121 tttccacatc tgcccggtaa cctttaccac cattgcatct ttaggtacat cacgcatatt
     3181 tataaatccg ttccagctta taaaaaacaa atacagaact attataatag gaacaacagt
     3241 ccatatgatt tcgatggtag tatttccctc tatattagaa ggtacaggat ttctttttct
     3301 gctgtattta aaaataaaat acaacattgc cactgttata atcagcagca gtattattga
     3361 tataccagtg attatccaaa aggtggtatc aacttcattt attatactag gtctcataat
     3421 ttaaaatctg tttatatctg aataaatatt catttaaaat ctgattgctt tttttattaa
     3481 aatctaaatt aataacaaat ttaatatcgg aacgtcaggt caaaaaatgt taaaattaaa
     3541 actattataa atatcagcat gcagactccg acaaatattt taaaaacctt attgtcaaac
     3601 ttcagatgca tgaagaaatt tatcacaaac atagttttga ctgttgcaat gatcagagca
     3661 actgtcagcg caagactgcc gagatcaata cccgctactg ccactgtaat ggatgtcagc
     3721 gaaagcagtc cgatccatac gagcacattt ataccgtaac tcggatgatg cgctacggcg
     3781 atgtgaccgt catcgttttc agaatgttct gagtgtgaag aatgatcaga attgtgatta
     3841 ctcattataa ttattattta aaattttctt taatgaatta aataaaataa cgggaacagg
     3901 aatatccaga taatatctac aaggtgccag tataatcctg aattttcaag cttctgaaaa
     3961 ttcgttgctg ttattacatc tttttttatc tgccacatta cgaatcctat aaatgcaagt
     4021 cctactacaa tatgaagtcc gtgcagacct gtcattacat aatataatcc gaaatacatt
     4081 acttcacctt cagacatatc ctgaagtgcg ggtccttttg gataaatacc gtgatggaat
     4141 tttgcacccc actcaaaata tttattcaca agaaagatca atccgcaaaa aaccgttacc
     4201 cacagaaaaa ataagctgag ttttttatta ctcttctgta atgctacaat agcaagtact
     4261 accgtaaggc tgctggtaag aagtataact gtatttatcg cacccatgaa tacatccagt
     4321 tcaccggctg caactacaaa agcatcagca tatatgaacc tgtaacttgc atagagaatg
     4381 aacaatccac caaagagaag tacttctgtg aataggaaaa gccacattcc cattttagac
     4441 ccgaagtcat ctctgtgtat atgaacttcg tggatctcct gatctgctgt gtcagagtta
     4501 tagctcaatt tgatacctcc tgtctatcct gtttgtgaat gtgagtatgg tcataaggac
     4561 ctgctgtgac tgtaggaatt tcatcaaaat tttcaagtgg tggaggagat ggtaccgtcc
     4621 attcgagagt tgtacctccc catggattat cttcggtaca cttctttcct tttctgattc
     4681 cgtttataag attaaatgcc ataaccaaaa ttcctgatac aagtatccat gacccgactg
     4741 ttgaaataat atgatagatc tgatattcag gcagataatc ataatacctt ctcggcattc
     4801 ccatatatcc cataataaac atagggaaat aaagagtatt aaatccgatt gctatccagt
     4861 aagctgttac cttagccact ttttcattat acattttccc gaacatcttt gggaaccagt
     4921 aatgtattga agcaaaaaat atgagtccca ttcctccaaa cattacgtaa tgaaaatgag
     4981 ctactacata ataagtatcc gtcagatgca gatctgtatt caaggcaccg agagtaagtc
     5041 ctgtaagacc gccgatagaa aatataaaga taaacaacat tgtaaaaagc attggtgcag
     5101 tcatttctat tgaaccttta taaagtgtcg ccacccagtt aaaaactttt atccccgatg
     5161 gcaatgcaac aaaaaatgtc agcaaagaaa atattactct tgatgtatcg ctgattccac
     5221 ttgagaacat atgatgtccc catacaagat atccgactaa tgcaattgca agactggaaa
     5281 ttgcgatcgg cacatatcca aagatcggtt tttttgcaaa tgtcggaatg atctctgaca
     5341 cgactcccat cgcaggcagt atcataatgt aaaccgccgg atgagaatat atccaaaata
     5401 agtgctgata taaaagcggg tcaccgccaa gcgcaggatc aaatactcca attccgaaag
     5461 ctctttcgag tatcacaagt aaaagtgtga ttccgattat aggagttgcc agcacctgta
     5521 tccaagaggt tgcatagact gaccatacaa acagcggcat tctgaaccac gtcattcccg
     5581 gacatctcag cctgtgaatg gtcgtgagaa aattcagacc ggtaagtata gaagaaaatc
     5641 cgagtataaa agcagcaaag accgatatgg aaacgtttgt agtagtcctg atactgtaag
     5701 gcgcataaaa tgtccagccg gtatcagcta ttcccgatcc tataaagagt gaaaatattg
     5761 ccagtcctcc gcctatcata taaagatacc atgacatcag attcagtttt ggaaattgta
     5821 cgtcctttgc gccaagctga atcggcaaca tgaagttacc gaataccgca ggcactccgg
     5881 gtattataaa aagaaatatc atgatcacgc cgtgcagagt aaaaaatgag ttgtaagtct
     5941 gagcactcat tatcgtctcg ccgggtgtaa gcatttcaag tctcatcagt acgccgatcg
     6001 tcattgctgc cagaaagaaa accgacattg cagacagata catcagccct atcctcttat
     6061 gatcggtcgt aagcagccag gccattatcc ctttatgctt tgtcctgaca tgataaaaat
     6121 caacttcagc tgcctttttg ggaacagcta tagttccgtt ggccataaat tatttaagta
     6181 ttttgtaaat ttgttttttt ctttttcaat gttaaatatc ctacaaatat tccgagcagt
     6241 aataatatcc cgccgcctgc aattcttgtt acattcaaaa catatttcct tccggccgga
     6301 tcataactga aacacatctt tactatctta gcaatagtcg gaacagattt accttctgca
     6361 gcttccgtca ctgccatttt cagatcaaaa ggcagatagt cagtaccgta taaatatctg
     6421 atcactttac cttcaggtga gatcatgatt agtgaagctc cgtgcatgaa atcagcaccc
     6481 tgtctcgcaa atttaaatcc gacagcgtct gtgatcttat gaatattcag actgtcgccg
     6541 cttagaaatc tccatgaccc gggagatatt tttttatcca aagtactcag atatgtttct
     6601 ttcttacccg ctgccaatgt aaaatcttcc gtctgatcaa aactgatcgt gacaatatcg
     6661 taatcaatac ccggctcaat gtctgcctga tcgatcactt tcgtaagccc gttcagaagc
     6721 ggtgtgcaga tccccggaca tctgaaataa acaaacgaaa atacagtcgg cttattgatc
     6781 agacttttta gatttaccgg atttccattc tcatcattta atataacatc atcaggtatg
     6841 aaatcaccta atttttccct gattcctata tcaggtaatt ttctttcttc accttcactc
     6901 tcctgagaaa acaaatttcc gtttatcaca gggaataaaa tgcatataaa taaaaaacta
     6961 attattaatt ttttcataac acattatttt ggtcagacct tattcacgga gaacagggtt
     7021 ctgagatatt tccagaccgt ttcggaatct gctgcggaga gctgatacac atcccctttg
     7081 aatcctttat ccaaaggatc ggtctttaaa aaattcataa actcttcatt acttgtcgcc
     7141 catttcatat cgtttgccag tgacagcatt gctgtttttt tatcggaaat tattttatca
     7201 agtattacaa ctgctgaatc actcttgtcc agttccattt ttataaggat cttttcaaga
     7261 tcatttttta atgtatcttg ctcaagtacc atcagatcca ttgcaagctc gaccggtatc
     7321 tgattcgcag cttttacgcc tgttgcaaga ctgtatttct catcaagctt cgtcagctct
     7381 tcaggtgtgt cttgcggata tagcggattg aatgtcctta tatactgaat gactgcaaac
     7441 ctgtctttag gcggaatagt gctaaagctt gccattcctg tgtttggaat tccttcctga
     7501 agtgtgacat acatctgcga aactgtctgt ccgtttttcc atgtctgttt tgcaggatct
     7561 ttaaaatttc ttggcggcgg atttaatgaa gttcccgcag gaccgttgcc ctcacctgtc
     7621 tcaccatgac agctctgaca acttgccgcg taaatatctt ttccctgaga aacaagatcc
     7681 ggtgacggag tgctgaaaaa gatcacgtcc acaggcggag tcatagttcc tttattaaca
     7741 ggcagcggag ttctcatgtt tgagtctgct gataaaagtc cgggggctgt aaagagcttg
     7801 ttataatcaa gtgtgctgac gtatttcgcg ccgagcatta cgaggaaaac aagcagcagc
     7861 atataaagca cgccgtagaa cttttccatg tcacggaaaa tatcgtatat gttgaattta
     7921 cctttgctgt tattattgtg tgacacttta taaaattaag attcttaaaa attaatttaa
     7981 atgaaaattt aaacccgctt caagctttgg atcacctaca ggtacaaggt tggatttcgc
     8041 agctctcatt ctgaatacag ttatgatcag tccaaatgca aacatcagaa atccgagttc
     8101 gctccatccg aatacaaatc cttctgtgaa atagctcggc attattatcc agtaaagatc
     8161 atatgcatga gcaaacagca gccatattga aattatagta agcagctttg gctttacctt
     8221 cgaactcctc ggcagcaata ttaaaaacgg tattacaaaa tgaacaaaga tcagaccgat
     8281 cgagaaataa gaccagcttc ctttcatcct catcattatc cagaatgtct cttcaggaat
     8341 atctgcatac catatcagca tgtattgtga aaatccgatg tatgcccaga agacattaaa
     8401 tccaaacagc agtgtggcaa agctgtaata attattgtcc tttatattac ttacaagata
     8461 accgttctca ttgagctgta ccgaacaaaa agtaccggct gcaaatgcag ctaccatcgt
     8521 acctgcaaaa tagtaaactc caaacattgt cgaaaaccag tgatactcaa gactcatcat
     8581 ccagtctatt gcaataaagg taatagtgaa cataaacaat atcgcaaatg ccgctgagaa
     8641 ttttatgctc tgctttgtaa gcaaaggatc tcttgtgtcg tcctgcttta aggaatttct
     8701 tataaagaga aaataaaata caacccagat caataatatt atcgctgttc ttattaaaaa
     8761 gaatggtaca tttaagtaag cagatttggc ctgcagcagc ggatcgttta atagtgtctc
     8821 ctcatgcgtc cagtgaaaaa gatcatgcat tccgaatata agcggaatga caagtatcac
     8881 aagcagaggt ataagcgctg caagaaattc agatattctt ctgaacggcg tactccaggt
     8941 agctccgacg agatattcaa gtgctacaag tgccagcgaa ccgacaccga tactcaccaa
     9001 gaacatatac atccagagat aatcaaagaa tgctctgtgc gtatccgtta caaacgataa
     9061 taccacgagt acaagaccga ccccgagaag tccggttccg agttttgcta caattggcgg
     9121 taagggtttt ttagtatagt ccattaaaaa ttataatata aaataattca aatacttttg
     9181 ataaatttat ttagctttct caagatcttc ctcgctagca tcttttgctc tctgaagcgc
     9241 tctgatataa tttactatcg cccatctgtc ttcttttgat acctgttcgg aatagctcgg
     9301 cattacattc tgcccgtttg tcatgacatg aaaaatattt ccgtcgggcc agttctttac
     9361 tttttcattg tgaagactcg gcggtttcgg gaactgatct ctaagtctgc cgtcgccgtc
     9421 acctgtatat ccgtgacacg gactgcagta aatattaaat ctgtcctgac ctctttcgag
     9481 agacttcact gtgaccggaa cgggatttga taaaagcttt acaactgaat ccggcattcc
     9541 ctgatattca taaggcacga atcctcttgc tacagttcct tccactggcg gtctcatgcc
     9601 tgattgattc gggaaaaaat cacttttgct ctgagcatcg atccttccct gctgccacat
     9661 ccagttgaat ggcacagttt cgtataaaac ataattcaat gtaacataag ctactccggc
     9721 tgtcatcaat cctgttatga taattgccat tgtaaacttc ttgtcaaaga gcggagtctt
     9781 ttttgttaga tttgattctc tgaaataaat atcctgaact tcataggcat tggtctttga
     9841 aaataaattc aatacttcat cttcaatata catctcatca cctgtttcga tgacgagtcc
     9901 gaatttatcc gaagtcacat gtttcataaa gtcagtgtca ttcagcggac tgttgagttt
     9961 cggcatttta ctgaatagta tcagcagacc aagcacagtc gcaataccgc aaagcagtac
    10021 agtagattca aatgtgatcg gaattgcacc cggcagtccg aagaaaggtt ttccgccgat
    10081 gatgtttgga taatcgatcg aagacatcca tatcatcatg accagtgcga gtatcgtacc
    10141 ggtgattccc attatgaatg aaacgtaacc gatctttggc ggaggtaatc ccattgctgt
    10201 atccattcca tgcaacggat agggagtata gatatcaaac tttttatacc cctttcccgc
    10261 cacgacagcc gcagcttcgg tgatctcgtt cggagtatta taaagagcgg ctttgccgta
    10321 aagcttgctg ctccttactt ccttatcttc cgcggtatca aactctttta tcttatttaa
    10381 aatgtttttt aacactgtat aaattttaaa ttttttgtga agacagccaa ttggatgtct
    10441 ctacaattca taatttttaa tttgtaattt ttaattgctc tttcaccgga tgcggatgca
    10501 cggtcggctg cgcaccaggc atgactgctt tgacttctga aattgaaacc aatggcagcg
    10561 tcttcaaaaa caataacagg aacgtaaaga aaaatccgaa acttcctaaa agcaatccca
    10621 gatccgtcag tgtcggcgtg aacattgccc agctcgacgg taaaaaatcc ctgtgcagcg
    10681 atgtcactat gatcacaaat ctctcaaacc acataccgac atttacaaga agtacaagta
    10741 ccattgtaat cggaatagac cttctgatct ttttaaacca gaatagctgc gggaatatta
    10801 cgttgcatga aaccatgatc cagtatgccc atgcataagg accgaatgcc ctgtttataa
    10861 aaatgaactg ctcaaatgta ttgccgctgt accatgctat gaaaaattcc attccgtatg
    10921 cataaccaac catcataccg gtcgcaagca ggatcttatt catcgtctca agatggttca
    10981 gagtgataat gtgctttaga ttaaatatct ttctgactat gatcaaaata gttgacacca
    11041 ttccaaatcc cgagaagata gcacccgcta caaagtaagg cgggaagatc gtcgtatgcc
    11101 atccgggaat taccgatacg gcaaagtcaa aactaacgat cgagtgaact gaaagtacaa
    11161 gcggtgttga aatacctgca agtatcagat aagctctctc gtaattgctc cagtttctgt
    11221 tggagtttgt ccatccgaga ctcatcacgg aatagatcgt cttctttact ttgctcgtcg
    11281 tcttgtccct taatgtcgca agatcaggaa taagtccgat cccccagaat aaaaatgaga
    11341 tagtgaaata agtcgatact gcaaatacgt cccagagcag cggtgagtaa aaattcaccc
    11401 agactccgtt catgtttggc aacgggatca gatatcctgc aagccatggt cttccggtat
    11461 ggactatcgg gaatataccc gcagtcataa cggcaaagat cgtcatcgct tcggcaaacc
    11521 ttgcaatacc cgttctccac ttttgtctga agagatagag aatagcagag atcaacgttc
    11581 ccgcatgacc tattccgatc cagaaaacaa agttgataat tccaaatgcc catccgaccg
    11641 gctgattatt tccccacatc ccgataccgt aataaatagt cagtccgagt gatacagctc
    11701 ctatcgtcag cgctgtaagt gatatcagaa aagctatcca ccagcccttt gtgggcttgg
    11761 tctcagtcgg cagtgcaatg acactgtcaa tgtccttaaa ggaaactccg ccttctacta
    11821 atgggatctc tttggtgtaa gtagcgtcaa gactcaatgt cgctcctgtt tttaaatgtt
    11881 ttcaaatttg aaaaatatac ttttgtaaat attaattatg aaaattttta cttgaatatc
    11941 ttttgcgcat attactttat taatatcatt ttttttattt ctgaaaaatt gcttgtttcc
    12001 attttgtaat aatatactcc gctcgaaaga tctgcgccgt tgaattccgt atcataagtc
    12061 ccggcatttc tgaattcatt tacaagcgtc tttatttcct ttccggtgat gtcatatact
    12121 ttgatagaaa tattcccgga tactggaatg ttgaatgtaa tctttgttac agggttgaat
    12181 ggattaggat agttctgtga taactcaaaa ctttcgggct gtgtattaat ttttgataaa
    12241 atattaaatc tcttatttat caaagcgctt aatatcccgt ttattcttgt ctgtttatat
    12301 tcaagatttt cgattcccga cccgcctata aaattatctc cctctcccgc agaaagtatt
    12361 tctttattta tcagcgcatg cattccttta gaagtgttta tgttattcag atatattgta
    12421 ttgtaatcgc ttatcgcttc ttcaatatta ttctgtttca ctttagtttt tattatgaaa
    12481 tcttccgaga ggtttctctg tgactcactg tatgaagtat cattaaaaat atttgaatag
    12541 taattttgga gcgcgccgta ttctgaaacc gttgaggttt ttttctcata acaattaaat
    12601 aatctggata aactcgaaac tacaaacgaa ctgcctatat tatccgaata atccgaaatt
    12661 atttccttgt agatctctat tgcttcagga taatctcccg aatgttcttt ctgataagct
    12721 tccagataaa gctcctgagc taagcccgga tcatctgatt cctgaaagaa tacagtatca
    12781 tataatccga atccaatgtc gtaaagagtg taattgttta cggatctctg accggacgaa
    12841 ttgtcatcat atggattaaa gagtaacccg tctgaatttt ctacattaat tttattcaga
    12901 tcgggagaaa gcgttcccca gtaattttcc cttacatcaa aagtatcgga accggtagga
    12961 ttcgttccgt ttatataata atcgttattt gttaaagtaa atctgttata tccgaatgcc
    13021 atattcggat aggattcatc ttcaaagaaa attcccgcat ttgatggcga gcctgtaaaa
    13081 tgattatcgc cggagatcca gtatttagag ccgcctgatg ttacgggatg catgtttgga
    13141 accgatgagt atgaaagaca gatattatca taatatgaac tgttaaaaac atttttcaac
    13201 agatcaggcg aagacagtaa agagtaataa gtataataaa aatcatttat aatattatat
    13261 tcaaacttac cgtatgaatc acttataaaa attcctgatt cagaatattg attaccgtta
    13321 acagtgtttt tagaaacgaa cggagagctg tttaatatag tcattccgtt tcctgaattg
    13381 ctgattgtgt ttccaattat gttgaaatat cctccgtcac aaagctcagc gtaaatcccg
    13441 tgatcaaaac cttttgtgag tatgtttgat gaaactgaat tatcctttat taaaattata
    13501 ctgcttccca ctgcatatat tgcgttattc aaaacatgac ttgtctgatt gataaaagag
    13561 caaccggaga tttcagtcga atacggaact tccgttcctt catcatcatt tttattcata
    13621 atatttatgc cgaagtctgc gtttttaatt acgcagtttt ttattgtatc ctgagagcgg
    13681 tgttttaaag aaataccgtt ccattttttt gttgaatcgg cggaagtaaa gaccgcatta
    13741 ttggcaatca ccttcgcccc gctgtcacac acaatcccgg agttctctcc aaacatcatc
    13801 tttgatcccg gcttcataat taaagaagtc gtatttcctc taagatgaaa attataacta
    13861 tttggcatga tcacaactgc gctgtcttcg aggatgattt gcgtgctgtc tttgatgaca
    13921 acatcattta cataagagca aaattcgtga atgccttttt caataatgat cattcccgga
    13981 cctctgacct ttccgccttc gttgcagaat ccggctccgt ttttaattct gatttcagag
    14041 ttgctgagga gagtcaggtt gcttttattt ttcagagtta atttattagt agaatcaaca
    14101 ataatttttg ctccattata taatatcaga tatgcagtat cttcgactat tagattatta
    14161 tagctgttta caggagttat catatcaaaa gttttattcg aattaatttt aagtgctgct
    14221 ttttttgtaa tgacaatgtt tgttggctcg tctattaaag tatcacgagt agaaatattt
    14281 ttatcaattg ttaaatttaa agtaagaact gcgcttctag gatctgtgaa tacttgattc
    14341 tgattcggaa tattagttaa gttgcaatca tatctagaat cttgtgggaa aataatatat
    14401 ggattactac cataattatc ataagaaaaa tttccataat tcttattcat tactcctatt
    14461 ccccaacgga tccatgattc tattttttta tcaacaaggg gatcatcgat atatttttct
    14521 tcagcatttg ctcctcccca atgaaaagat attttaccat catcataacg atcataataa
    14581 attaaacaat cagcattccc aaaatttgga taacctgcat caaattcaat tgtacaagta
    14641 tcaatcaaac tttgatcaga aaattttctg aattcaattt tatatttacc gaatgcaaca
    14701 gctccgcgat ttccagaatc cgtgtcagtt aagtcaaacg ctaatcctcc ataactatta
    14761 ggagataata ctatcatatt tttttttaca ttagtagata attcaatata aacacaatta
    14821 ttaaaatcat atctaaaagg ttgaccattc tgatttaatt tagattttaa attatattta
    14881 taatccccat tgaatataag actaacagga aatattttaa tttcaattgt aatgtcagag
    14941 ttattttcaa ataatatttc ttctataaat gaattcgaat ccttactttt agcttgatta
    15001 acaaatatta ataagcctat taaatacaat acaattttca attttctata atattattta
    15061 actaaaatca ttagtatggt tttcattaaa attccattaa tgaaaaaatt gcaaaagtaa
    15121 tatccacttc caactttatc agaattaaat aaaaattcat ttgatccggt cacaatattg
    15181 ctatattttt tttcataaac aattttacct aaaatatcat taacattaat tctaagtgtt
    15241 gaaggtttag ctaaaatgaa tttaatttga gtactgctat taaatggatt cggataattt
    15301 tgagaaatga catatccatt aatatcgaat gaaatcgaat tgttatttat actaagaggt
    15361 tgtgagaaca ataaactatc tgttcttaca ataaaaatat ctctgtttac tgtaacatca
    15421 ttttttgaag tacctaaaaa tattatatct ctgctatttc tgtcagtttg aattgatctt
    15481 aacaatgtat aatcacttaa ataaataatt ctttgcttaa ttatattccc aaacgaatcc
    15541 gtaaggtata ttcttgcgtt tcttccggtt gttgagtcga catctgatgt aaaaacaaat
    15601 ttattgttat ttgagatttt aaagtcctgt aaatattcgt tactgtaata attgataata
    15661 ttagaaaata ctattttccc gctggtgtct gtttttatta atatcgtttg cgaaagagaa
    15721 tttgaagagt ctgaagtgga acctgctata atgtaattat tcatatggaa aataattttt
    15781 ttaaaagatg taaatacacc attgaaataa aattctcttt cccgaataat ttcacctgat
    15841 gaatttaatt taattaaata cccgtctata ggtgaaattt cgctatcaga tttatatcca
    15901 gcagctacat aatagttatt ttcttctgta acagaataaa actctatact gaatttacct
    15961 gataatattt tcttccaaac aatattgccc tctgaatcga ttttaattaa ataaccgttt
    16021 cttgtggact gaagaattct accgcatgcg atatatcctc catccatagt tttatttatc
    16081 tcatacaata ttacaccggc ttcattaaaa ttcttttccc aaattatatc accgcggtta
    16141 ttaactttaa tgaagaaaat atttgaaaac gaacctgtaa ttatacaacc tccgtcaccg
    16201 gttgaagcgc aggtaaaagg ttgtttgccg ctatgatttt gaagtgtctt ttcccaaatc
    16261 gtgtcaccat taggttttat tttcattata taaattaata caccgttagg cccggattgt
    16321 gtcgaataac cgactacata aaaatttcca ctatctgcct cacatatatc aactccattg
    16381 tcattcagat tttgattatc atataatctt tcccatgaaa tagactgcga cttcacctga
    16441 ccccatcccg ccgccaaaca aaacaatatt aatatcccaa aaatcttttt cataatctta
    16501 agtaaaaatt gatttcaatc tctcaaattt aatctgcatt tttcgtcaat acaactttga
    16561 agacttttat taaaatgtca tattcataaa actcgtaact gcctatttca aaattattca
    16621 ttgtataaat tgactattaa tgacccgcag attctgtttc catttcatat acatttctaa
    16681 gttttgctaa ataagtaata ttcggcttta cctttatttc ttcaagtaca tggtaagcta
    16741 atttgctttg attcaattta tataattctg attctttatc attctgatct ccgaatgaga
    16801 tcgcatttgt attacatgca tcctgacagg ctgtctttac attcgatccc tttacctgtc
    16861 tgttctcctg aattgccatc tgcttctctt ccattattct ctgcagacag aatgtacatt
    16921 tctccatcac acctcttgac cttactgtca cttcaggatt ctgcataagc gtgaatgact
    16981 cttcttcctg tatagcgttt ttgaaattat ctctgaagtt gaagtaattg aatcttctta
    17041 ctttgtaagg acagttattc gaacagtatc tcgtacccac acatctgtta tatgccattc
    17101 cgtttattcc gtcagtgctg tgagtggtcg ccgctaccgg gcagacgttt tcacacggcg
    17161 cgaaatcaca gtgctgacaa agcatcggct ggaaactcgc cttcggcact tccggcgtac
    17221 cggaataata tctgtcgatc ctgatccagt gcattgctct gtgattgttt gactcttctt
    17281 ttccgaccac gggaatatta ttctcgacat tacatgcagc tacacattca ttgcagccga
    17341 tacatttatt gagatcgatc accattcccc atttggtgcc ggtatgttta tactgctcgt
    17401 tgatactgcc gacttttagc ttcgcagtct cttcttctga atattttgta tgtctgtcac
    17461 tcaggaaatt cggattctct ttatattgta aataagtacc ttcctgtatt atctctcttc
    17521 tgtactgaat gtctttataa agcggctcgt ctatcggata tgaatcctgt acagacacta
    17581 tctcatatgt accacctgcc ttttctatct tcgcgtcatt ataaaaccat ttactgagtt
    17641 tcggagattt tgacatcatc acatttgcat tgaatcctgc atcttcacct actgtgccgc
    17701 agacttttct gccatagcca agctcaattg cgatcactcc gtcagccatt ccgggctgca
    17761 caaatactgg gatctccatc ttacctgctg atgtcgtaat attaatatta tcattagaat
    17821 ccactccaag agcttctgca ctcttcacag aaatcgctgc atagttatcc cagactattc
    17881 ttgaaactgc tttcggcaat tcctgcagcc agccgttgtt tgcatatctc ccgtctccga
    17941 gcgaaccatt cctcaatagt aatagagaaa atccttcacc cgatgtcatt tgatttgtat
    18001 ttgaaataaa tgaagcggga ttgaatgctg atttcgctgt tgaagtgttt gttaaaattg
    18061 gtgctgtatt atttgttttg gttgaatctg ctgttaaagt agttgctgtt tttgtagaat
    18121 ctgctgttgc agaatttgtt gtctttgtag aatctgaagt gaccgctgct gttgtcagct
    18181 ttggctttcc cgacgccatt acaattccgt cattaagacc tgcataccat gccttagtaa
    18241 aatcgctgtc cgtacctgcg gaagaaagta ttcccgtctt ccagttatcc attacaaatt
    18301 tatggtaatt atcgatgttg tatgaatcgc ctgttccgct gatccaggtc atcagtacat
    18361 cttccttctg ccgtgtatta taaagcggcg ctatgaccgg ctgctgcaga ctgtaaaaag
    18421 tatttctcgt ctgataatct ccccatgact cgaacatcga attcacaggc agtacaaaac
    18481 ttccgatctc ggaagattca tttaccgatt cccctaatgt aataattgtc tttgcttttt
    18541 ttaccgcttc tgtatatcca agatctgttg gtaaatgata aacaggattt gtatcaaaat
    18601 gaatcactac cgctgtcttt cctgaattca gatccgaaac gagattctcc atttcttcac
    18661 gtgtactcag cggcatcact gttacatttg attcgtcttt tctgtaaagt gcagatccgc
    18721 cgatgacttc gttcaataga tttaccgcta tctgcgttga ctctggaagc attgatcctg
    18781 ctgtgacaat tgacgatccc tgattctttt tgagatcaga aaccaaatgt gaaattactg
    18841 cttcggataa attattttta gcagcaaatg atttcatgtc atacttctgc agctttgaag
    18901 tgatacctgc atcatcagca aaattcgata ctctctcttt tccagtcagt tcatttaata
    18961 aacacatcac aaactcttct attgcatccg ttttcaatat cattctgtaa tcagaattca
    19021 gtccggtgat cgaggcattt ccctcgactg tatataatct gttgaatgtc tttccgtcta
    19081 tcgcatctct tccctgcgta aataatctcg ccgtctctac cttgtttcct tcacttccta
    19141 aaaaatctga ctcgagtgac aatacaatat ttgccttgtc ccagttgatt aacggcaggt
    19201 ctttattacc ataacatttt tcccatgcat tctttttttc cgattcatca aataattcat
    19261 aagaataaat ttttgcagtt ggataaattt ttacaaactc atcaaaaagt tttttctgcg
    19321 aaggtgagtt tatagtgtgt gttacaattg aaatttcttt acctgaagat gcagcacttt
    19381 taagctctgc gattattttt tcatctgcat ttttccattc aatgtcattc ggacttactc
    19441 tgttaattct ctctaccgga ttttttaatc tttccggatc ataaagactc atgacacttg
    19501 cctgaccgat agcacagatc tttcctttgc tgatgggatg atcaggatta ccgtccactt
    19561 taattggtct gccttcaaga gttttaatta aaattccgca tgcagaagaa cagcctgtac
    19621 aagtcgatgc ataataagtc ggatttccga ttgttataga ttcgggtttg ctgttataag
    19681 gaatgatctc acctctgtct ctgtaattcg aacagcccga agctgcgact gcagccgaag
    19741 cgctaagcag tgccaggaat ttccttcttg tgaattttga aaatgcactc atgtcgggtt
    19801 tttgaagttc acccggaaga aactctaagc tcttttcttt tttgaatgaa ggatctttat
    19861 acaattctct gaagcttctc cagtaattta gatcctttgg ctcttcagct ttattagtgt
    19921 caggaggaat aaggagttca gccgttgttt ctaccgggct gttctctatg attctctcac
    19981 ttatatcaaa taccttattg tcaggtttgt tcatataaaa taattattca tgtttatatt
    20041 atgaatttct attgtttgat tgttttgaat ttcgttttac tgcatttgga ttcgaaacaa
    20101 tttttatttt cgatactggc tttgaagatt tatgatcaga tcctgattta aaaaaaccaa
    20161 atggaatgac cgaaacagct gctaaacctg cagctgcaag tcctgtgaat ctgaagaact
    20221 tttttctgtt gattttttcc ggggaatttc cggaagctgt gatattgtcg ttgctgttac
    20281 tgactggtaa attttttttc ataaattaat tttatttatc tgtgacacga tgaacagttt
    20341 gtcggacctg tgttgatctt ggatttatct tttccgggca taatattttt tattgtctta
    20401 tccggatttc tgtgacagtc aagacatgct cgcatgctga aatccttgac ctgttctaca
    20461 acgtccattc ctgctacatc tccgtgacag ttcgaacagt cgattccttt gttgacatgt
    20521 acgctgtgat taaaatatgc aaagtccggt aagagatgta ctcttttcca ttcgatcgga
    20581 actccccttg tataataatc cgtaagtttt attatttcag gtctgtcctt tcttacaatg
    20641 gtatgacaat tcatacacac ttttgctgaa ggaacatttg cttttcttga attctccaca
    20701 tcactgtgac agtatctgca gtctattttc atatcgccgg catggagctt gtgcgaataa
    20761 ttgatcggct ggatcggctg atatccgact ccgactcttt cagcatatga aacgtaatat
    20821 gtgatggcaa aaaccgacag gactacaaag attaagatag gtaaacgaac cttgaggttg
    20881 taatctatta aaaaattttt cattaagatt tttttggaaa aataatgcta caaatatatg
    20941 aaatataaga tttgatttct atgacttatt tataccaatt tctaaaaaca attaaaaatt
    21001 aagaattaaa aattaggaat tattactgca catctttttt aaaaaattca aaacatttaa
    21061 tcttaaatca taattttact atttagaatt cttaattcct aattctcaat tcttaattgc
    21121 tttttaatgt ccaccgatct tacagcactc atttaaatta tcataagcag ttttatctgc
    21181 cggcttgtca tttgcctgat atccggctga tactattgca ccttctatta cagtgagatc
    21241 cgttttgctg gcgtcataat caaccataca ttttttctta tcaacgctga cattaacagc
    21301 aattactccg tcgactttgt tcaatgcatt tttaatattc ttcgtacacg taccacattg
    21361 tatagtcggg acatcgaccg caagtaattc accttttact ttatgaggtt cttctccggc
    21421 tgttttattt tcatctttgg aagttgaagt tgtctcttca gatttttttg tatccgaatt
    21481 ttcactttcg gttttattat cagaatctcc gcaccctgca tatataaatg ctgagaatac
    21541 caatgctaat attgttaagt ttatcttctt cataagttta ttgattaatt aattggttaa
    21601 aataatttat tcagacagga attgtaactc aattttttag tctgattact gaagcggcta
    21661 agaatttttt gaagatgaaa caaataggtt gtaatatatt ggttgtaaat tttagtttaa
    21721 gttaaactta cttttttcgg tgttgagttt ttgcaatttg ttatttgctg tttctttcgg
    21781 catcaccgga cacttcccgc ctgcactttc gggcaaaagg gaggagtatt aaaagattca
    21841 taatgctttt tgattctggc tataccattc tatgaatgat agaacgatta taaattaaat
    21901 atttcaaata tcattttttc ttctcgccat aagtttccaa aatattattt atcacttcat
    21961 atgattcatt caaactcaat tttgtaaggt caactgtctt atattctctc ttcacatttg
    22021 cacctcctat agctccgccg attattccac caatcaatcc gccaatcaga gttgctccca
    22081 ctacttcacc aaaatttaat gaaatatccg gatgcccgcc ggaagcttga cttaataata
    22141 ttcctaatcc aagacttata cccccgccaa ttgccgctcc tgtcagcaat cctgatgcgg
    22201 gatttgattc tgacatatat cctacattta ttatatcttc gagatatatt ttttgaacat
    22261 tacccaaatc tgtaatggca agtgaatcgg agatcacctt ttcaaatgta atttttctta
    22321 aattttttct gaccttagtg gaatcgaaat atgatatatc acaatttttt acctgagaat
    22381 atgctgcagc atttagaaag agaattgaga ttaatattac caaaacaatt tttatgttca
    22441 tctttaaatt tttttaagcc aatttattta aattcctttt gaattacaat tcacagaaac
    22501 tgagtaagta tttaaaatta tataacagtt ctaaaatatc agaatttgct ttaatgattt
    22561 tcaatcctta tttttataca gcatacattt cttaaattta accaaaataa aaatatgttc
    22621 tcagtaatct caagattagt aaatggcgta aaaggcacta ttacttcaag cttaaaaggt
    22681 gtttctgata ttgcaggtac agtggttgat gttgtaaaaa agatcaccgt taatacgatc
    22741 aaagatacag cagatataat caaagaagga attaaacttc cggcgtctat cgttcagggc
    22801 gctcttcagg gagtagctga aatcggagcc agcgtaatcg gcgctgtaaa aggtgtagtt
    22861 aacggaactg ttcagggagt tatggaatca ggcggttcac aggacgacgc agtcagaggc
    22921 gcagtagggc aggcaatgca cagtgcaaag gaactcggtg ttgatatcgg agagactgca
    22981 cttgaatcag tgaaaggttc agtggaagct gtaagctcag ttggcggaaa tactgtcgaa
    23041 gctacaaaat cagcagtcat ggcagcaatg gaagcagcaa aagaaatcgg cgcagaggca
    23101 gttgattccg taaagaatac acttacttca ggagttgacg gggcaaagca gatcatagat
    23161 tctattaaaa gttaatagtt aatagttaat agttaataga ttattaattt aagtcttttt
    23221 aaaaaaaacc attttgaatc actttcaaaa tggttttttt ttataaaaat ttttagtttc
    23281 taatttttaa ttatttattg ct
//
LOCUS       DAORVQ010000027        22961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_821_length_22961_cov_4.381778, whole genome shotgun sequence.
ACCESSION   DAORVQ010000027 DAORVQ010000000
VERSION     DAORVQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 22961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22961
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_22961_cov_4.381778"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..3297
                     /locus_tag="PLX80_03960"
     CDS             229..3297
                     /locus_tag="PLX80_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19985.1"
                     /translation="MRNFNKFVLILLAHFIIVGSALSETYYVNDATGSNGNSAALAKN
                     FMTPWKTVQYAIDNAAVVAGDNIVVAEGTYSGFIVSKRVNIIGVWKGSNPLTNTIVNS
                     TVTLTASGGNPSSRMVLKNMRIVSPIGDAVDARESFFTLENVFASSGQTYGLRFNADI
                     SDALLESCNFDNSTYCGIYFPTLFSTRKFRMYNSTASNNAYWGIAAFQRANNPTVIDD
                     VEIIQCAFENNNPSNQQQGHQIYFEKLSNSVFRNISVVTPPGNIWIGIDINLLARTDY
                     TDIQIFNSNVKRATPGSGIWIQARNDLINPPASLDTVFLRGLSFENCDTLIAFNRQVL
                     NMVVEKCDLSNYLVYGLVNYSDQGGTINASDNKWKNGGTPDTTVISGGLLTSGNPIIS
                     FMPSTVGIFIGMGIQGAGIPPGTTVIGKSPNTITMSANATANAFIPSIGFAFNFATST
                     DLVRTSLNTINYFSTKQYSIINQDNQDFPDLASAIAGTTSGGTIWNVPSGIIPGNTVV
                     DRNLTLISSGSGFLSPMSLTTFQNLSITNATMTMRSDFAVADNFSPNRINIGLHNTLV
                     LNGSITPGGIIEGGMNSDLVISGAGPSTGLTTVENGIRNFRMNRASGISLLDNIWIHR
                     LLFLSNGVVSLGNFDVNLGTHTTTFNPNPSNSYVGTNGSGNLNKYFEASTPGLYNLVI
                     GNNGAANAKIFLSNATFAPDAYVSAKTINTIHPNNGCGTDYLNRYWTLSQNGISGYNV
                     WNQFGYQDADVVGTEASLNGAKWNGFQWTSYTPVNAAANNFVIPTSNSFGDYTAGSPT
                     CLGGSNTIANLKVLLQGAYIGGGNMRTSMNGFGLIPLSQPYNTTQFSYNGTESVTSIP
                     AGVVDWVYVELRDTDNGSAILNGKRAGFVKSDGSIVDLDGVSPLKFTSVAAGMYYVVV
                     GHRNHLPVMSANKTTLNAVSTVYDFATDLTKYYGGDAASLTGGLFGLYAGDANRSFIV
                     SAADYTVVTNNLLQANYNDGDLNLNGTVTSADYGFVTNSLTKASQVPNYQ"
     gene            3319..4419
                     /locus_tag="PLX80_03965"
     CDS             3319..4419
                     /locus_tag="PLX80_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19986.1"
                     /translation="MKQIKFLVLILLTVFAFRYSVSQNTVTNTITNFIISNNITKFSF
                     DVYSLRTSAPDFRMGSSSYFFSYTQGTFDNVQITNVNPKYTVSSSTNSYNEMDAFTYS
                     SGKVSIQVFYNSNGPGDLISNDPGSSGLGEKIATINLDITNQNNMPDLKWNSINTAVV
                     DPFFQSAILTNNGEFNQPLPVELSSFTSVVSGNSVDLNWSTSMEINNRGFEIERKTES
                     TDWKKIGFVEGSSNSNISVNYVYNDRSLSKGIYNYRLKQIDFNGNFEYFDLHSEVNIG
                     TPAAYSLSQNYPNPFNPSTKINFTIPENSKVSLKIFDISGKEVKTLINNDFRNADYYT
                     ININLGDLSSGVYFYTLTGSNFIQTKKMTLLK"
     gene            4482..5573
                     /locus_tag="PLX80_03970"
     CDS             4482..5573
                     /locus_tag="PLX80_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19987.1"
                     /translation="MKKIYKLSFLFVLLFTAFNLNAQNTATVTATNFEIINNGANFRF
                     DVHALRTSVAAFDMGNSSFVMDYAPGALNNAVLSNQNSRFSTGNYNSMTSAVFFGQQV
                     ALQINFNFGGPGEEITNVPGSTGFGERLATITMDILQNVQANLVWDPSSSDIVTTNFT
                     PVTSTYNGNYNGTLPVELTSFVSAISNNSVTLSWATASELNNKGFDIERKPLVENSTW
                     SKIGFVDGNGTVNETKNYSYRDQNLATGNYSYRLKQVDFNGNFEYFNLQNEVIIGIPQ
                     KFALDQNYPNPFNPSTKINFSLPVDSKVTLNIYDLSGKLVQTLINNELKSANYYTVQF
                     NGANLSSGAYFYSLVTGENSDTKKMILIK"
     gene            5680..7083
                     /gene="fumC"
                     /locus_tag="PLX80_03975"
     CDS             5680..7083
                     /gene="fumC"
                     /locus_tag="PLX80_03975"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQY19988.1"
                     /translation="MEYRIEHDTMGEVKVPSDKYWGAQTQRSVENFKIGGQRMPVEVI
                     NAFAVLKKATAAANNICGVLAEDKMNAIMNVCDEISDGKLESEFPLVVWQTGSGTQSN
                     MNVNEVIANRAHVLSGGKLTDEKKILHANDDVNKSQSSNDTFPTAMNIAAYKMLIETT
                     IPGIRLLADTLSEKSRKFMNVIKIGRTHFMDATPLTLGQEFSGYAAQLEHGLRAIENT
                     LKHLSELALGGSAVGTGINVPENYDTLVAEKISEFTGFPFITAPNKFEALAANDAIVE
                     ASGALKTAAVSLMKIANDIRMLSSGPRSGIGEIFIPENEPGSSIMPGKVNPTQVEAMT
                     MVCSQVIGNDTAITVGGMSGHFELNVYKPMMIYNFLVSARLIGDACVSFNNNCVSGIE
                     PNYKAINENVERSLMLVTALNPHIGYENAAKIAKKAHKENKTLREAAIELGLLTDEEF
                     TEKVDPSKMVGKITSDK"
     gene            7087..7665
                     /locus_tag="PLX80_03980"
     CDS             7087..7665
                     /locus_tag="PLX80_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY19989.1"
                     /translation="MQIFNDEVFTEFPVIETKNLILREFNSEDAEVLFQNYSDKEVMK
                     YFGRELFKDFSEAEIKIENTNSDFKNKLGIRWAVCLKSDKKQMLGSCGYWRILKEHLR
                     AEIGYELSKEFWGKGIMTEALQAIIEFGFTGMKLHSIEANIDPENLGSVKILEKLGFV
                     REGYFKDSFLFQGKFTDTSSYSLINITDSFNK"
     gene            7662..8324
                     /locus_tag="PLX80_03985"
     CDS             7662..8324
                     /locus_tag="PLX80_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQY19990.1"
                     /translation="MKKYKFIKFEHERLETEESMKRGETFYKLMKKRRSIREFSPEIF
                     DVKLLEYAIKTAGTAPSGANKQPWKFIIVESPEIKKEIRIAAEKEERESYERRMPQSW
                     LDDLAPLGTDWHKEFLEIAPYLIVVFKVDYLKVDNELKKHYYVNESVGLATGILLASL
                     QNMGLVTLTHTPSPMNFLQKILNRPSNEKPYLLIPVGYPSVNAEVPDISKKDVNEIME
                     IV"
     gene            complement(8492..8983)
                     /locus_tag="PLX80_03990"
     CDS             complement(8492..8983)
                     /locus_tag="PLX80_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQY19991.1"
                     /translation="MKFIQFLIIVILLSVSVNSFSQELIPANKETLDKIISDNKDKVI
                     LFNYWATWCKPCVDEFPALMEIQKNYKDKDFKLIFVSLDFGNDFKDEITTFLKKNGVD
                     FDTYYNNFDKDEELLNYVSKDWEGAIPGTFIYDKDGKLKKSLIGKHDYDEFKTAIDKY
                     LTN"
     gene            complement(9010..9597)
                     /locus_tag="PLX80_03995"
     CDS             complement(9010..9597)
                     /locus_tag="PLX80_03995"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQY19992.1"
                     /translation="MKKLNLILTFVIALIFTANGFSKTASDGFSIGQPVADFTITNYD
                     GADYTLSKSGANYTVIIFMSTQCPFVQPYTDRINNLVSEFGSNGVAIWGINSNKTEST
                     EDVIAHAKDKGYNFPILKDNNNVVADLFAAEKTPEVFIIKNSDMTLVYHGRIDDDKEA
                     DKVTVSDLKLTLASLNSDQAVPVSQTKAFGCSIKR"
     gene            9885..12011
                     /locus_tag="PLX80_04000"
     CDS             9885..12011
                     /locus_tag="PLX80_04000"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY19993.1"
                     /translation="MSFLTEKANSQGFNSIVTPDGIYVIAVGDNGLVFRSSNSGNSWS
                     SYTINSENLSSVSSIENDVWIAGENGNIHKTGKTNSAVSTFTTGSTSNVNSITFVNNS
                     IGYICGDNGTVYKTLNGGESWSLSNSGIAGVSLNSISFLDEMKGVVVGDEGYAYLTDN
                     GGMTWTAQEMVTSYNLLKTKYFDEGIVITGEFGVMKTKLNSGIWNNVDTKINSDFRGI
                     SGTSFYNVHVCGGGGFIRNNSNSSADFLNFEINPMMANLVDIFYYDNNLGFAVSSLNK
                     AIIRTTNGGISWELPSGTNVVQNWTQKSPGGSGIGNNLCMHPTDRNSTFVVYGNKVYV
                     SRDKGEIWNQIATISIGTRAHSFYVSPVDTNIWLSAITGSGTDKVVRSTDYGQTWNTI
                     ISYDFSTYGQPLEMDQNDPTNFYFVPANSSGTGLFRSTDNGATFNLAAPYNNSSIGSP
                     CDLIVMWDNSDIMYLGDDGADIYKTTNRGVNWYLVKPGSSSEIPSMCNSVFDQTICYA
                     TSWSSSQVFKTTNIGENWNITSNNSGSGWGSDVCREDPTLVLTGNYGSQSYLTTNGGS
                     NFFNVNSGLSGAGAGILVPDRSYLLNMQTGSLYKMNIIYDVITNINENTASISVPENF
                     ELYQNYPNPFNPVTNIKFSIPQNGNVSLKIFDRLGREVEILSEGFRNAGTYEINFDAS
                     NLSSGVYFYKLVSNDFVSTKKMLLVK"
     gene            complement(12054..13649)
                     /locus_tag="PLX80_04005"
     CDS             complement(12054..13649)
                     /locus_tag="PLX80_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19994.1"
                     /translation="MKKVINEKVRLTGSLSTNGHINSKSSKKSLHTSNLIKVLSTFSK
                     KELNEFEKFVSSPFFNNQSTLVRLFNEIKNYYPEFNSIDFTKENLFSIINPGKKYNDT
                     LFRKYFSNLAKLSEDFLVCIDIKSNDDRRQTSLLDQYDKRILYPMFEKLSQQINERLD
                     HEGITNVSFYYKNFREELKASFNIRTNNLQRLNNDLKNAHKYILLHLLLSSTVYSNMM
                     LVNKKSFKDADLENTFEGFFETFDLIKYLESRNDLSRSESLFVDLCKYDILLMKDPYV
                     KENLTSMNDVLLKMTSHLNKNLLYTFFSHLNIYYIINIENGKKEYLRDLFENYKLMIS
                     KDLYISEGRGYINFSEYRSILLYALKLKEYDWAEEYINKHKTYHNPETQKNISNFSFA
                     YLNFEKNNYDEALNFLSKVKRDNIIIKLDTEVIYMILYFEKGYFDSALSLAETTFQSL
                     STNKVLSKDVLINQLKFIKYFKAVIKIDSVRNDRTSIEILYNTISSEPQFRRKDWLLS
                     KLELLVKKNQKIIPKNLQNNKTD"
     gene            14144..14554
                     /locus_tag="PLX80_04010"
     CDS             14144..14554
                     /locus_tag="PLX80_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19995.1"
                     /translation="MAHINYQYFNNVFTFITAPVSNYYLILKYYNAIETRSSVPVSFA
                     ANINYYEFINMQNSAFGGNMIQKGERWCIFNGDTKQEGIIDLDDMQQIDNDAYNFVQQ
                     QPYISDLNGDNIVDITDIVIADKNAFNFVSSVTQ"
     gene            14847..15560
                     /locus_tag="PLX80_04015"
     CDS             14847..15560
                     /locus_tag="PLX80_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19996.1"
                     /translation="MYKGLIRRCKIMILSSSMFVLLIGIAMLFFPGGNLVDKTSIHYN
                     FFFNSLSDLGNSITITGKDNTGSKILYITAFGSLGLVMIYYSRIWWGMGSDVINMKVV
                     GYTSKVFLILSGLGLIGIAFTPVSTLFEMHMIYFEIMLFAYSIWIILTMIIQNSNDKL
                     RKLFTMNFFNLVIIIVYILIMLNYDKPEKEDDFQFYAFSQMIAILSIVVNLFIQSVGL
                     MHFLKTTDYRRSGIRDFYV"
     gene            complement(15635..15889)
                     /locus_tag="PLX80_04020"
     CDS             complement(15635..15889)
                     /locus_tag="PLX80_04020"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQY19997.1"
                     /translation="MKIEIYSKKNCHLCEVMFNTVNESILELNTGYDFNVEVIDIETK
                     AELQKYKLKIPVLMIDGKMFAKYEVNKDKLKKKLKSGNFN"
     gene            complement(15892..16701)
                     /locus_tag="PLX80_04025"
     CDS             complement(15892..16701)
                     /locus_tag="PLX80_04025"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQY19998.1"
                     /translation="MYPKLFEIGPVPVYSYGLMLGIAFLVGSSLFSKELKRNGMDENI
                     GVAVTFLCLIGGIVGSKFFYIIEEWNFGSDRALSTYFTFDTIFSPSGLTFYGGLAFAI
                     ILVLIYCKVKKISVLKMFDMMSPSAAIGYGIARIGCHLSGDGDYGTEVNGTFWEFIGY
                     SYSNGTVPTAEGVLVHPTSVYETIVSVFIFYFLWKNRAKFKTPGIIFSYFLILSGIER
                     ILIEIIRLNPRIIFGLSQAQIISVVMILGGTMLLFKLLTKEKDLDLKNTKV"
     gene            complement(16691..17113)
                     /locus_tag="PLX80_04030"
     CDS             complement(16691..17113)
                     /locus_tag="PLX80_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY19999.1"
                     /translation="MRTKQVRPDKLEFEYELYVSREFDKTKDKEFIQFDFRTIKVFEN
                     FEYKINLDSAIDTENKILKFNIEGLSAPILDISKAGPACHKFKFYEFKNVDYDLILLK
                     YGKNKCKFEIKISPKNIKIKSTPEKKFLEIFTEKYNVS"
     gene            complement(17128..18375)
                     /gene="hisS"
                     /locus_tag="PLX80_04035"
     CDS             complement(17128..18375)
                     /gene="hisS"
                     /locus_tag="PLX80_04035"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQY20000.1"
                     /translation="MLIQKPKGTYDLLPEKARQRYFLETKVRENFEIYNFSEIRTPTF
                     EKTELFKRGIGEETDIVSKEMYTFKNDEFTLKPEMTAPVIRAYIENNLNNESPLQKLF
                     YITNMFRRERPQAGRFREFSQFGAESIGSNDPYCDAEMIILADSIIRSFGIDDSIIKL
                     NTIGTAAERDVFFVEFKNYLSNYLNELSPDSQRRFVINPLRILDTKNPGDLKILKDAP
                     VLYNFLNDETKGNFEKILDILTVNGIEFETDNRLVRGFDYYTSTTFEFISDALGAQNA
                     LLGGGRYDLLIQQLGGKPTPAIGFAAGIERLLMIMESKELLPDNNDELKLFIVSMGEE
                     SKKFSFKMLENLRNIGVKCETDFLNRSVKSQMKEANRSKAEFVIVIGDEEIKSNSVKL
                     KKMSDGSEVTVSGFENIKFFLDK"
     gene            19057..19560
                     /locus_tag="PLX80_04040"
     CDS             19057..19560
                     /locus_tag="PLX80_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQY20001.1"
                     /translation="MGEIKNLSESDAIEKVKELAEEIDICMLCTYENGRMMARPMSTQ
                     KVDDEGNVWFLSDKSSDSNRQIENNNEVDLLYSKDYYKYLALHGSAEILFDKDKIKEL
                     WKKHAEIWFTEGVDDPRISVIKFNFDDGHYWDNKNGRIVQLTKMAASLLTGKTMDDGI
                     EGDLENL"
     gene            complement(19654..21087)
                     /locus_tag="PLX80_04045"
     CDS             complement(19654..21087)
                     /locus_tag="PLX80_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY20002.1"
                     /translation="MKIKFKLSIFLVFIHIISGSAKELEGDLIIKNVNIVNVLDGSVS
                     RSMDILIIRDRITEILNHDNSSTYKAFEVINGEGQFLIPGLWDMHTHSWWAYEYFFPM
                     LLVNGVTGIREMWGEQTEIDKIRIGITNGSIKGPDIFSAGPIIDGSPPLWKGSDSADS
                     PERGREFVRKQKAEGADFIKVYTFLERAVYFAIADECKKQGISFGGHIPLKISLEEAL
                     NSGQISLEHFFGILEFCSSQKESLYSSMINENNEEKNDTLFEPRKFSTFLNRMKFETE
                     TFDRSKLPELVTLLSQSNSWICPTLVAAEGSINRSLPDFKPMEEIKFMPDFAVENWNP
                     KVDTSKADLQNRNRQIESDWYALVTSVFRTMLDGDVKFLAGTDFPNPYCYPGYSLHEE
                     LRLFVEKCGFTPLEALQTATINPAVFLKMDNELGTVEINKKANFVLLGSDPLENINNT
                     KNINGIILRGKFHAQSELKDSLKLKTR"
     gene            complement(21093..21347)
                     /locus_tag="PLX80_04050"
     CDS             complement(21093..21347)
                     /locus_tag="PLX80_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQY20003.1"
                     /translation="MLIQKPKGTNDLLPEKARQRYFLETKVRQNFEIYNFSEILTPTF
                     EKTELFKRETGEETVTVSKQMYTFKNDEFIFLLPMLNVFD"
     gene            21643..>22961
                     /locus_tag="PLX80_04055"
     CDS             21643..>22961
                     /locus_tag="PLX80_04055"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20004.1"
                     /translation="MKKILFLFILLALNINSYSQWIESSRPSPAFPLFSFSFPSANTG
                     FAVGYGNTMIKSTNGGVNWFNISFFNTTANDLRSVFFINENTGWMCSTSDTVFYTTNS
                     GSSWTPQISFVSDANQVYFTNSSTGWILSTPNLFRTTNAGVNWTKINTQMGYDFYFFG
                     ENTGWRTLYSGGSSSIQKTTNGGVNWMTKHTTSNFRVIYSIDFVNENTGWAGGYREHI
                     LKTTDAGDSWIQQRDMDNSAGFYSMDFINENTGWAIGDAGMSVYTINGGSVWNQVPLN
                     PGRAQIKFINSNTGWIVGSKIYKTNTTGLIYKNLSCNTLIEGFYNDKSNTINSDSVRI
                     YLRNGNAPYSMKDSAISLMNSDGSGTYNFLNAANGENYYLVIKHRNSLETWSSSTQIF
                     IANNLNYDFTVSSTQAFGSNQILIDSSPVSFAVYSGDVNQDGTIDLS"
BASE COUNT         7784 a         3595 c         3775 g         7807 t
ORIGIN      
        1 tttgttagga ttagaattat tttatagtaa taatttattt gctgcaaagt tcagtaatat
       61 taaaaaaaaa tagatattaa aaacaattga aatttgatat ttattgaatt attttagaac
      121 ctaaattatg tacctaaaaa atataaaatt aatattttca agaaaaatcg atatttaaat
      181 tattaatcat ataaaaattt ttaaaaatta attaaacaaa gaacgcagat gagaaatttt
      241 aacaaatttg ttttaatttt actggctcat tttatcatag taggttctgc attatcagaa
      301 acttattatg tcaatgatgc aaccggaagt aacggaaatt cagcagcctt ggctaagaat
      361 ttcatgactc catggaagac agtacaatat gcaattgata atgctgctgt agttgcagga
      421 gataatatcg ttgtagctga gggaacgtac tctggcttta tagtttcgaa gagagtcaat
      481 atcataggtg tttggaaagg ttcaaatcct ttaacaaaca caattgtgaa ttctactgta
      541 actctaactg catcaggagg taacccttcc agcaggatgg ttctcaaaaa tatgaggatc
      601 gtatcaccaa ttggtgatgc tgttgatgca agagaaagtt ttttcacttt ggaaaatgtt
      661 tttgcttcat ccggtcaaac ttacggatta aggttcaatg ctgacatttc agacgcactt
      721 cttgaatcct gcaattttga taattcaact tactgcggaa tatattttcc tacacttttt
      781 agtacaagaa aattcagaat gtataattct actgccagta acaatgctta ttggggaatt
      841 gctgcatttc agagagctaa taatcctacc gttatagatg atgttgaaat tattcaatgt
      901 gcttttgaaa ataacaatcc atctaatcaa cagcagggac atcagattta ttttgaaaaa
      961 ttatcaaatt cagtttttag aaatatatca gttgttacac ctccgggaaa tatctggata
     1021 ggaattgata ttaatctact tgcccgaaca gattatacag acattcaaat ttttaactca
     1081 aatgtaaaaa gagctactcc tggaagcgga atctggatac aggcaagaaa tgaccttatt
     1141 aatcctcctg cttcacttga tactgtattc ttaagaggat tgagttttga aaactgtgat
     1201 actttgattg catttaacag acaggtattg aatatggtag tggaaaaatg tgatcttagt
     1261 aattatctgg tttatggatt agtcaattat tctgatcagg gtggaacaat taatgcaagt
     1321 gataataaat ggaaaaacgg cggcacacct gatacgactg tcatttccgg tggactttta
     1381 acttcgggaa atcctattat ttcatttatg ccttcaacgg ttggaatttt tataggaatg
     1441 ggaatacaag gtgcaggtat tcctcctggt acaactgtaa taggtaaatc tccaaataca
     1501 attactatga gtgcaaacgc aactgcaaat gcatttattc catcgatcgg ttttgcattt
     1561 aattttgcaa catctacaga tcttgtaagg acttcattaa acacaataaa ttatttttca
     1621 actaagcaat attcaattat aaatcaggat aatcaagatt ttcctgatct ggcttctgca
     1681 atagcaggaa caaccagtgg cggtactata tggaatgttc catctggtat aattcccgga
     1741 aacacagttg ttgacagaaa cctaacactc attagttcag gttctggatt tttaagtcca
     1801 atgagtctta caacttttca aaacttatct attaccaatg ctacaatgac tatgagaagt
     1861 gattttgcag ttgctgataa tttttctcca aacagaataa atattggact tcataataca
     1921 ctcgtactca atggctcaat tactccgggt ggaataattg aaggcggaat gaattcagat
     1981 ctggttataa gcggagcagg tccaagtact ggtttaacta cagttgaaaa cggcataaga
     2041 aatttcagaa tgaacagagc ttcaggaatt tctcttttgg ataatatctg gattcatagg
     2101 ttattatttc tttccaatgg agtggtttca ttaggaaatt ttgatgtgaa tcttggaaca
     2161 catactacta cttttaaccc gaatccttct aacagttatg ttggaacaaa tggcagtgga
     2221 aatttgaata aatattttga agcatccact ccgggacttt ataatctggt aataggaaat
     2281 aacggcgcag caaatgcaaa aatatttctt tcgaatgcaa cttttgcacc tgacgcttat
     2341 gtaagtgcaa aaactattaa tactattcat ccaaataacg gatgcggaac tgattattta
     2401 aacagatact ggacattaag tcaaaacggt atttcaggat ataatgtctg gaatcagttt
     2461 ggataccagg atgctgacgt agtcggaact gaagcttcac ttaacggagc aaaatggaat
     2521 ggtttccagt ggacttctta tactcctgtt aatgcagcag ctaataactt tgtaatacca
     2581 acttcaaatt catttggaga ttatacagca ggttctccaa cttgtctggg tggatcaaat
     2641 acaatagcaa atctaaaagt tcttctacag ggtgcatata ttggaggcgg aaatatgaga
     2701 acttcgatga acggctttgg attgattcca ttgagtcagc cttataacac cacacagttc
     2761 agttataatg gtactgaaag tgtaacaagt attcctgcag gtgtagttga ctgggtttat
     2821 gtcgagctaa gagacactga caatggatca gcaattttaa atggtaaaag agcaggattt
     2881 gtaaaaagtg acggaagtat agtggatctt gatggtgtaa gtccattgaa attcacaagt
     2941 gttgctgcag gaatgtatta tgtagtagtt ggtcacagaa accatcttcc tgtaatgagt
     3001 gctaacaaaa caactcttaa tgctgtcagt acggtctatg attttgctac agatcttaca
     3061 aagtattacg gaggtgatgc agcttctctt acaggcggtt tattcggttt gtatgcagga
     3121 gatgccaaca gatcatttat tgtatcagca gcagattata ctgtagttac taataactta
     3181 ttacaggcta attataacga cggagattta aatttaaacg gaactgttac ttctgcagac
     3241 tatggatttg taactaatag tctgacaaaa gcttcacagg ttccaaatta tcaataacag
     3301 taattttaaa aaagctatat gaaacaaatt aaatttttag tattaatttt attaacggta
     3361 ttcgcattca gatattctgt atctcagaat accgttacta atactattac aaattttata
     3421 atatccaata acattacaaa attcagtttt gatgtttact cattaagaac cagtgcacct
     3481 gattttcgaa tgggaagttc atcatatttt ttcagttata cacaaggtac ttttgataat
     3541 gtccaaatta caaatgtaaa tccgaaatat accgtttcca gttctactaa ttcatataat
     3601 gaaatggatg catttactta tagcagcggt aaagtttcta ttcaggtttt ttacaacagt
     3661 aacggacctg gcgatttaat ttcaaatgat cctggttcat caggtttagg agaaaagata
     3721 gctacaatta atctggatat tacaaatcaa aataatatgc ctgatcttaa atggaattct
     3781 ataaatactg ctgttgtaga tccatttttt caaagcgcta ttttaactaa taatggtgaa
     3841 tttaatcagc cgttacctgt tgagcttagc agctttactt cagtggtttc aggaaacagt
     3901 gttgatttaa actggtcgac atcaatggag atcaacaaca gaggatttga gatcgaaagg
     3961 aaaactgaaa gtacagactg gaaaaaaatc ggatttgtag aaggaagcag taattccaat
     4021 atttctgtta attatgttta taatgataga tcattatcaa aaggaattta taattacaga
     4081 ctaaagcaaa ttgacttcaa tggtaatttc gaatattttg atttacattc cgaagttaat
     4141 attggtacac ctgctgcata ttcattgtca caaaattatc caaacccttt taatccatca
     4201 actaaaatta attttactat tccggaaaat tcaaaggtaa gtcttaagat atttgatata
     4261 agcggtaaag aagttaaaac tctaatcaac aatgatttcc gtaatgcaga ttattacaca
     4321 ataaatatta atctgggtga tctatcgagc ggagtatatt tttatacatt aacaggatcc
     4381 aatttcatac aaacaaaaaa aatgacactt ctaaaatagt gtcttaaatc atataatttt
     4441 ttaaaatgag tttaaaaaaa atatttattt caggaggtaa aatgaagaaa atatacaaat
     4501 tgagtttttt atttgtattg ctgtttactg catttaatct aaatgcacag aatacagcaa
     4561 ctgttacagc tacaaatttt gagatcatta ataacggagc aaatttccgt tttgatgttc
     4621 atgctctaag aaccagtgtt gctgcttttg atatgggcaa ttcaagcttt gtaatggatt
     4681 atgcaccggg tgctttgaat aatgcagtat tatcaaatca gaattccaga ttcagtacag
     4741 gaaactataa ctccatgaca tcagctgtat tttttggtca gcaggtagct ttgcaaatta
     4801 attttaactt tggaggaccc ggagaagaaa taacaaacgt tcccggctca acgggttttg
     4861 gtgaacgtct tgcaactatc acaatggata tacttcagaa tgtacaggca aatctggttt
     4921 gggatccatc atcatctgat atcgtaacca ctaattttac accggttaca agtacttata
     4981 acggaaacta taacggaaca cttccggtcg agcttaccag ctttgtatct gctattagta
     5041 ataacagtgt tactttgagt tgggcaactg cctctgagct aaataataaa ggatttgata
     5101 ttgaaagaaa acctttagtt gaaaattcta cctggtcaaa gataggattt gtagatggta
     5161 atggtactgt aaatgaaact aaaaattatt cttacagaga tcagaatctt gcaacaggaa
     5221 attattcata cagacttaag caggttgact ttaatggtaa tttcgaatat tttaatcttc
     5281 agaatgaagt gataattggt attcctcaaa agtttgcttt ggatcagaat tatcctaatc
     5341 cattcaatcc aagtacaaag atcaatttct ctttaccggt tgattcaaaa gtcacactta
     5401 atatttatga ccttagcgga aaattggtac aaacacttat caataatgaa ttgaaatctg
     5461 cgaattatta cactgttcaa tttaatggtg ctaacctgtc cagtggagct tatttctatt
     5521 cacttgtaac aggagaaaat tccgatacta aaaaaatgat actgatcaaa taaattttta
     5581 atattcattt tacctgatat tttaagtccg caaagaattt aacactcttt gcggattttt
     5641 taattataaa ttcttttaat attcaaaatc aaaataaaaa tggaatacag aattgaacat
     5701 gatacaatgg gcgaagtaaa agttccttca gacaaatact ggggagctca gactcagcgt
     5761 tcagttgaga attttaaaat aggcgggcaa cgtatgccag tggaagtgat aaacgcattt
     5821 gctgttttaa aaaaagctac tgccgctgct aacaatatct gtggtgtact tgcagaggat
     5881 aaaatgaatg ccattatgaa tgtctgtgat gaaatatctg acggaaaatt agaatcagaa
     5941 tttccattgg tagtatggca gaccggctca ggtactcagt ccaatatgaa tgtaaatgag
     6001 gtcatagcaa acagagcgca tgtgttaagt ggcgggaaac ttactgatga aaaaaaaata
     6061 cttcatgcaa atgatgatgt aaataaatct cagtcttcta atgatacttt tccgactgca
     6121 atgaatattg cagcttacaa gatgctgata gaaactacaa ttcccggaat aaggctgtta
     6181 gcggacacac tttctgaaaa atctaggaag tttatgaatg tgattaagat tggcagaact
     6241 cattttatgg atgctactcc gctaacactt ggacaggaat tttccggata tgctgctcag
     6301 cttgaacatg gtttgagagc tattgagaat actcttaaac atttgtcaga gcttgcttta
     6361 ggaggatctg cagtcggtac aggaattaac gttccggaaa attacgatac actggttgca
     6421 gagaaaattt ctgaatttac cggttttcca ttcataacag ctccgaacaa attcgaggca
     6481 cttgcagcca atgatgcaat agttgaggct tcaggagctc ttaaaactgc agctgtaagt
     6541 ctgatgaaaa ttgctaacga cataagaatg ctttcatctg gtccgagaag cggaataggt
     6601 gaaattttta ttccggagaa tgaacctggt tcatcgatca tgcccggtaa agtaaatcct
     6661 acacaggttg aagcaatgac aatggtatgt tctcaggtga ttggaaatga tacagcaatt
     6721 actgtgggag gaatgagcgg acatttcgaa cttaatgttt ataagccaat gatgatctat
     6781 aattttcttg tttcagcaag actcattgga gacgcatgtg tttcttttaa taataattgt
     6841 gtgagtggaa ttgaaccaaa ttataaagca attaatgaaa atgtggaaag atctctaatg
     6901 ctggtcactg ctttgaatcc tcatataggt tatgaaaatg ctgccaaaat tgccaagaag
     6961 gctcataaag aaaataaaac tcttcgcgaa gctgcaatag aacttggtct gctgaccgat
     7021 gaagagttca ctgaaaaagt agatccttct aaaatggttg gtaaaattac ttctgataaa
     7081 taaattatgc agatctttaa tgatgaagtc tttactgaat ttccagtaat cgaaacaaag
     7141 aatcttatat tgagggaatt taattctgaa gatgctgaag tcttatttca aaattattca
     7201 gataaagaag tgatgaaata ttttggaagg gaattattca aagacttttc tgaagctgaa
     7261 ataaagattg agaatacaaa ttcggatttc aaaaataaac ttggaatcag gtgggctgtt
     7321 tgtcttaagt cagataaaaa gcaaatgtta ggaagttgtg gatactggag aatattaaaa
     7381 gagcatttaa gagctgagat cggatatgaa ttatcaaaag aattttgggg gaaaggaatt
     7441 atgaccgaag cacttcaggc gattattgaa tttggattta caggaatgaa actgcattct
     7501 attgaagcta acatagatcc tgaaaactta ggatctgtaa aaattctcga aaaacttggc
     7561 tttgtaagag aaggttattt caaagacagt tttctgttcc aaggtaaatt cactgatact
     7621 tccagctatt cattgatcaa tataactgac agttttaaca aatgaaaaaa tataagttta
     7681 ttaaatttga gcatgaaaga ttagaaactg aagaaagtat gaaaagggga gaaacttttt
     7741 ataaattaat gaaaaaaaga agaagcataa gggaattctc acctgaaata tttgatgtta
     7801 aattacttga atatgcaatc aaaacagccg gtacagcacc ctcgggagcg aataagcagc
     7861 cctggaaatt tatcatagtt gaatcacctg aaattaaaaa agagatccgc atagccgctg
     7921 aaaaagaaga aagggaaagt tatgaaagaa gaatgcctca aagttggctg gatgatctgg
     7981 ctccgctagg aacagactgg cataaagagt ttctcgagat tgcaccttat ttgatcgttg
     8041 tctttaaagt ggattatctg aaagttgata atgaattaaa aaaacattat tacgtaaatg
     8101 aatctgtagg acttgcaaca ggtattcttt tagcttcttt acaaaatatg ggtttggtaa
     8161 ctcttactca tactccttca ccaatgaatt tccttcaaaa gatactgaat cgtccttcaa
     8221 atgaaaaacc atatctctta ataccggtag gatatccatc tgttaatgca gaagtaccgg
     8281 atatttctaa aaaagatgtt aatgaaataa tggaaattgt ttagataaaa tacattaagc
     8341 accagatgta actgctatat atgaaagcag atagcaatac tcacttaaat ggttaaataa
     8401 ctaacgcttg ttgagggatt gattgaatat tgaaaataaa aaacccggca ttaaccgggt
     8461 ttttttatat tttattaatt atgaatcaat tctaattcgt tagatattta tctatagcag
     8521 ttttgaattc atcataatca tgtttaccta tcaatgattt tttgagttta ccatccttat
     8581 cataaataaa agtccctgga atagctcctt cccaatcttt acttacataa ttcagcagtt
     8641 cttcatcttt atcaaagtta ttgtaatatg tatcaaagtc cacaccgttc ttttttagaa
     8701 aagtagttat ttcatctttg aaatcatttc cgaaatcaag tgaaacaaaa atcagcttaa
     8761 aatccttgtc cttataattt ttttgtattt ccataagggc aggaaattca tctacacaag
     8821 gtttacacca cgtagcccag taattaaaca aaattacttt atctttatta tcactgatta
     8881 ttttgtcaag agtttcttta tttgcaggta ttaattcctg tgaaaatgaa tttacactga
     8941 ctgataaaag aattactatt attaaaaatt ggatgaattt cattgattaa aaataatttt
     9001 aaagaaaatt tatcttttaa ttgagcaacc gaaagcttta gtttgactaa caggaactgc
     9061 ctgatctgaa ttcagacttg caagtgttaa cttaaggtca cttactgtta ctttgtctgc
     9121 ttctttatcg tcatctattc ttccgtgata cacaagagtc atatcactgt tttttatgat
     9181 aaatacttcc ggagtttttt cagcagcaaa aagatctgca actacattat tgttgtcttt
     9241 taaaatcgga aaattatatc ctttatcttt agcatgtgca attacatctt ctgtagattc
     9301 agttttattt gaatttattc cccagattgc tacgccattg ctgccaaatt cagatacaag
     9361 attatttatt ctgtcagtat atggctgaac aaacggacat tgtgttgaca taaaaattat
     9421 cacagtgtaa ttagctccag atttacttag agtataatct gcaccatcat aatttgtaat
     9481 tgtaaaatct gcaaccggct gtccgataga aaatccgtct gaagcagttt ttgaaaaacc
     9541 attagctgtg aaaattaaag cgattacaaa agttaaaatt aagtttaact ttttcattat
     9601 agcctccttg ttttatatta attattatta aaatattgaa tattacgtat gccggagtaa
     9661 acgacatatt tttttaaata gtttcgtaaa attataattt tatttctaaa ataaaagtta
     9721 aaactattta aaaataacag aaaatatttt tttcataaaa agtccttgta atttaatatc
     9781 attgaacata ttttgaaaca atccaaaaat attaaaaacc attattaaaa accattaaaa
     9841 agatgaaaaa acttctaaaa tgtttttttg taataattat atttatgtct tttcttacag
     9901 aaaaggcaaa ttctcaggga tttaacagta ttgttacccc tgacggaatt tatgtaattg
     9961 cagtagggga taacggactg gtattcaggt catcaaattc cggaaactcc tggtcaagct
    10021 ataccattaa ttcagaaaat ctcagcagcg tttcgtcaat agaaaatgat gtctggatag
    10081 cgggagagaa tggtaatatt cataaaaccg gtaaaacaaa ttctgcagta tctacattta
    10141 caacaggatc aacatcaaat gtaaattcaa ttaccttcgt aaataattca attggttata
    10201 tctgtggtga taatggtact gtatataaaa ctttaaacgg tggtgaaagc tggtctctat
    10261 ctaacagcgg tattgccggt gttagtttaa attcaatttc atttcttgat gaaatgaaag
    10321 gagttgtagt aggtgatgaa ggatatgcat atctcacaga taatggaggt atgacatgga
    10381 ctgcacagga aatggtgaca agttataatc ttttgaagac taagtatttt gatgaaggga
    10441 tagttattac aggtgaattt ggtgttatga aaacaaaatt gaacagcggg atatggaata
    10501 atgtcgatac aaagattaat tcagatttca gaggaataag cggtacttca ttttataatg
    10561 tacatgtctg tggcggcgga ggttttatta gaaacaattc caacagcagt gctgactttt
    10621 taaacttcga aattaatcct atgatggcta accttgttga cattttttat tatgataata
    10681 atcttggttt tgcagtcagc agtttaaata aagcaatcat acgaactaca aatggaggaa
    10741 tttcttggga acttccttca ggtactaatg tagttcaaaa ctggacgcaa aagtcacccg
    10801 gcggaagcgg tataggaaat aatctttgta tgcatccaac cgacagaaat tctacattcg
    10861 ttgtgtatgg aaataaagta tatgtcagca gagataaggg tgaaatctgg aatcagatcg
    10921 ctactatttc aattggtact agagctcatt cattttatgt aagtcctgtc gatacaaaca
    10981 tctggttatc tgctattaca ggttcaggaa cagataaagt cgtaagatcc acggattatg
    11041 gacaaacctg gaatacaatt atttcttatg attttagtac ttatggtcag cctttagaaa
    11101 tggatcagaa tgatccaaca aatttttatt ttgttcctgc taattcttca ggtaccggat
    11161 tgttcagatc tactgataac ggggctacat tcaatcttgc agcaccttat aataattctt
    11221 ccatcggatc tccttgtgat ctgatcgtaa tgtgggataa ctcagatata atgtatttgg
    11281 gagatgacgg agctgatatt tataaaacta cgaacagagg tgtaaactgg tatcttgtaa
    11341 agcccggatc ctcttctgaa attccttcaa tgtgtaattc tgttttcgat cagacaattt
    11401 gttacgcaac cagctggagt agttcacagg tttttaaaac tacaaacata ggtgaaaact
    11461 ggaatattac atcaaataat tccggcagcg gatggggatc agatgtatgc agagaagatc
    11521 ctacacttgt attgacaggg aattacggct cgcagtctta cctaactaca aatggcggat
    11581 caaatttctt taacgtcaat tcaggactca gcggtgcagg ggcaggtatt ctggtgcctg
    11641 acagaagtta tctacttaat atgcagaccg gcagcttgta taaaatgaat atcatatacg
    11701 atgtgataac aaacataaat gaaaatacag cttccatttc tgttcctgag aatttcgaac
    11761 tttatcaaaa ttatccaaat ccttttaatc ctgtaacaaa cataaaattt tctattcctc
    11821 aaaatggaaa tgtttctttg aagatatttg acagattagg tagagaagta gaaattttat
    11881 ccgaaggatt tagaaatgca ggaacgtatg aaattaattt tgatgcatct aatttatcat
    11941 caggtgtata cttttataaa ttagtatcaa atgattttgt ctcgactaag aaaatgctgc
    12001 tggtaaaata aaaatagttt tttatataca atataaaacc ggctgaatat atttcagtcg
    12061 gttttattat tttgcaaatt ctttggtata attttttggt tctttttaac taataactca
    12121 agtttagata aaagccagtc tttccttctg aactgaggct cagaagaaat tgtattataa
    12181 agaatttcta tcgaagttct atcattcctt acactgtcaa tcttaattac agctttaaaa
    12241 tattttataa actttaactg attaattaaa acatcttttg ataatacttt attggtcgaa
    12301 agagattgaa aagtagtctc agccagtgaa agtgctgaat caaaatagcc tttttcaaaa
    12361 taaagaatca tatatattac ttcagtgtcc agtttaataa ttatattatc tcttttaacc
    12421 tttgagagaa aatttaaagc ttcatcataa ttattttttt cgaaattaag ataagcaaaa
    12481 ctaaagttcg aaatattttt ctgagtttca ggattatgat atgtcttgtg tttatttata
    12541 tattcttctg cccagtcata ttctttgagt tttaaagcat aaagtaaaat actcctgtat
    12601 tcagaaaaat taatatatcc tctgccttca gaaatgtaca gatctttact tatcattaat
    12661 ttatagtttt caaaaagatc tcttaaatat tcttttttac cattttctat atttattata
    12721 taataaatat ttaaatgtga aaagaatgtg tacagaagat ttttattcaa atgtgaagtc
    12781 atttttaaca atacatcatt catacttgta agattttctt taacataagg gtctttcatt
    12841 agaagtatat cgtatttgca aagatctaca aataatgatt ctgatcttga aagatcattt
    12901 ctcgattcaa ggtatttgat caggtcaaaa gtttcaaaaa aaccttcaaa tgtattctca
    12961 aggtctgcat ctttaaaaga ttttttattt accagcatca tattactata aactgtactg
    13021 gataaaagta gatgcaataa aatatattta tgtgcatttt tcagatcatt atttaatctt
    13081 tgcagattat ttgttctgat attaaagctg gctttcaatt cctccctgaa atttttgtaa
    13141 tagaacgaca cattagtaat accctcatga tcaagtcttt cattaatctg ttgtgacaat
    13201 ttttcaaaca ttggatacaa aatcctttta tcatactggt caagcagact ggtctgcctc
    13261 ctgtcatcat ttgatttaat atctatgcaa accagaaaat cctctgatag ctttgctaaa
    13321 ttagaaaaat atttccgaaa caaagtatca ttgtattttt taccgggatt tattattgaa
    13381 aataaatttt cttttgtaaa gtctattgaa ttaaattcag ggtagtaatt ctttatttca
    13441 ttaaaaagcc ttacaagagt tgattgatta ttgaaaaatg gagacgaaac aaatttctca
    13501 aattcattaa gttccttttt tgaaaatgtg cttaatactt ttattaaatt agatgtgtga
    13561 agagattttt tagagctttt agaattaata tgcccgttag tacttaacga accagttaat
    13621 ctaactttct cgtttattac ttttttcatg ttaaatttaa attaatcaaa ccccaaattc
    13681 gaaataaata taaatttatt ttgtgttatt ctatcaaagg aaagagtttc tttctttgaa
    13741 atttcaattg atttttttta aaaatcaaat tacagcaaat ctcaaattgt taatagttgt
    13801 tttaaaattt tacttttaga taaaaataat ttgatagaat gtatatactt tatttacaaa
    13861 aaaaaatctc taaaattaac ttaaattgaa cttaaatttt attataaaaa acactttcta
    13921 ttgtaaacaa agtaataact tagtcagttt tgatatgttt ttaattaata tttaaatttg
    13981 aatgtgaaga atattttaat cttaattcta caaaacatta aaaggataat atttttataa
    14041 attggaatat tgaagtaatt taaatccgga ttcaaaagtg aaaatcagca taatttttct
    14101 tttgagaata tattcttaca ttttaaattt gtaaagtctt ttaatggcac atataaatta
    14161 tcaatatttc aacaatgtat tcacatttat aactgctcca gtcagtaatt attatcttat
    14221 actaaaatat tataatgcaa tagaaacccg gagttcagtt ccggtttcct ttgctgccaa
    14281 tattaattat tatgaattta ttaatatgca aaactcagca tttggaggta atatgattca
    14341 gaagggagaa agatggtgca tatttaatgg tgatacaaaa caggaaggta ttattgatct
    14401 tgatgatatg cagcagatag acaatgatgc ttataatttt gttcagcaac aaccttatat
    14461 ttcagatctg aatggtgaca atattgtaga tattactgat attgtaattg cagataaaaa
    14521 tgcgtttaat tttgtctctt cggttacaca atagattccc cacactttta tttatagcgg
    14581 agtaaataac tccgcttttt ttttatggaa aacattaccg ctaattaaat atttagtttg
    14641 tatcttcaaa atacagttta gtattgatgt aaaattaagt atcttagcta agttaaaatt
    14701 taaaaacttt aaggtacaaa tattgatttt tacaatgttt aaaggaactt tgcaaatctg
    14761 atccccctat gtttttaaac gtaaattttt gcaaatttaa aattaatttt tattgaatat
    14821 tttttatcac ttccatttta ctgcaaatgt ataaaggact tatccgcagg tgtaaaataa
    14881 tgattctgtc atcttcaatg tttgtattgt tgataggcat tgctatgctt tttttcccgg
    14941 gcggtaacct cgtggataaa acttcgattc attataattt tttttttaat tcattaagtg
    15001 acctgggaaa ttcaataaca attaccggta aagataatac cggttcaaaa atattatata
    15061 ttactgcttt tggaagtctt ggacttgtta tgatctatta ttcgaggatc tggtggggga
    15121 tgggctcaga tgtgataaat atgaaagttg tcggttatac tagtaaagta tttttaattt
    15181 taagtggtct aggcttaata ggaattgctt ttactccggt aagtacatta tttgaaatgc
    15241 atatgatata ttttgaaata atgttattcg cttattcaat ttggatcata ttaactatga
    15301 tcatacaaaa cagtaatgac aaacttcgta aattgttcac gatgaatttt ttcaatctag
    15361 taattatcat agtttacatt ttaataatgc tgaattatga taaacctgag aaagaagatg
    15421 attttcaatt ttatgctttc tcacaaatga tcgccatttt atccattgtt gtgaatctgt
    15481 ttattcagtc agttggactg atgcatttcc tcaaaactac agactacaga agaagcggca
    15541 tcagagattt ttatgtctga tctttttaaa taaattaatt ggaggtgagt tatattgtaa
    15601 ttaaaatttg ttcggatcca tttttatttc ttccttaatt aaaatttccg ctcttcaatt
    15661 tctttttcaa tttatcctta tttacttcat acttggcaaa catttttccg tctatcatta
    15721 agaccggtat ttttaatttg tatttttgaa gctcagcttt agtttcaata tcgataactt
    15781 caacattaaa atcataaccg gtatttaatt caagaatact ttcattaact gtattaaaca
    15841 ttacttcaca tagatgacaa ttctttttag aatatatctc tattttcaaa actaaacttt
    15901 agtatttttc agatcaagat ccttttcttt tgtaaggagt ttaaatagca gcatagtacc
    15961 acccaggatc ataacaactg aaattatctg agcctgactt aatccgaata ttattcttgg
    16021 attcaatctg attatttcta tcagaattct ttcaattcct gacaatatta aaaagtaaga
    16081 aaaaataatt ccgggagttt tgaatttagc tctgttcttc cagaggaaat aaaatataaa
    16141 tactgaaact atagtttcat aaacagatgt aggatgaaca agtactcctt cggcagtcgg
    16201 aaccgtacca ttcgaataac tgtatccgat gaattcccag aacgtcccgt tcacttcagt
    16261 accgtaatct ccgtcaccgg acaaatgaca acctatcctt gcaattccat aaccgatagc
    16321 agcactgggt gacatcatgt caaacatttt tagtactgat atttttttta ctttgcaata
    16381 aattaaaact aaaataattg caaatgctaa tcctccataa aaagtaagtc cagaaggtga
    16441 aaatattgta tcaaaagtaa aataagttga caatgctctg tctgaaccaa aattccattc
    16501 ttctataata taaaagaact ttgaaccgac tataccgcca ataagacaaa gaaatgttac
    16561 agctactcca atgttctcat ccattccgtt tcttttaagt tctttactga ataaagaact
    16621 cccgactaaa aatgctattc caagcatcag tccgtaacta tatacaggta ccggacctat
    16681 ttcaaataat ttaggataca ttatattttt ctgtgaatat ttccaggaat ttcttctccg
    16741 gtgttgattt aattttaata ttttttggtg agatctttat ttcgaattta catttgttct
    16801 ttccgtattt taataagatg agatcgtaat caacattctt aaattcataa aatttaaact
    16861 tatgacatgc aggtcctgct tttgaaatat caaggatcgg agctgacaaa ccttctatat
    16921 taaattttaa aattttattt tctgtatcaa tagctgaatc taaatttatt ttatattcaa
    16981 aattttcaaa aacttttata gttctgaaat caaactgaat gaattcttta tcttttgttt
    17041 tatcaaactc acggctgaca tacagctcat actcaaattc cagcttgtca ggtcttacct
    17101 gtttagttct cataagaaat aaaattttta tttatctaaa aaaaacttaa tattttcaaa
    17161 accactcact gtcacttcgg atccgtccga catttttttt aactttactg aattactttt
    17221 aatctcttca tcgccaatga caataacaaa ttcggcttta gacctgtttg cttccttcat
    17281 ctgagacttt acgcttctgt taagaaaatc agtctcacat ttgacaccaa tattcctaag
    17341 gttttctaac attttaaatg agaacttttt actttcttca cccatgctta ctataaataa
    17401 tttcaattcg tcattattat caggcagaag ttcttttgat tccatgatca tcagaagtct
    17461 ttcaatgcct gctgcaaatc caattgcagg agtcggttta ccaccgagct gctgaatcaa
    17521 cagatcatat cttccgccgc cgagcaaagc attctgtgcg ccgagtgcat cggatataaa
    17581 ttcaaatgtg gtcgatgtat aataatcaaa acctcttaca agtctgttgt cagtttcaaa
    17641 ttcaattcca tttacagtaa gtatatctag tatcttttcg aaattacctt tggtttcatc
    17701 attcagaaaa ttataaagta caggtgcgtc ttttaaaatc tttagatctc ccggattttt
    17761 ggtatcgaga attcttaaag gattaattac aaaacgtctc tggctatcgg gggaaagttc
    17821 gttaagataa ttactcaaat aatttttaaa ctctacaaag aaaacatccc tttccgctgc
    17881 agttccgatc gtatttaatt ttataataga atcatcaatt ccaaatgatc tgattatact
    17941 gtcggcaaga attatcatct ctgcatcaca ataaggatca ttgcttccga ttgactctgc
    18001 accaaattgt gagaattccc tgaacctgcc ggcctgcggt ctttcccgtc ggaacatatt
    18061 tgttatatag aaaagctttt gaagagggga ttcgttatta agattattct caatgtatgc
    18121 tctgatcaca ggtgcagtca tttccggctt tagagtaaac tcgtcattct taaaagtgta
    18181 catttctttt gacacaatat cagtctcttc gccgatccct cttttaaata gttcagtctt
    18241 ctcaaaagta ggcgtacgta tctctgaaaa attatagatt tcgaaatttt ctcttacttt
    18301 tgtttccaga aaatatctct gacgtgcttt ttcaggaagt agatcgtaag taccttttgg
    18361 tttttggata agcattggca aaaatataat tttaatgtca ttaaataaac acaattacaa
    18421 tgttcaatgt ttaatgttca atgctgaatt ttcgatgtta ttattaaatg atcaatttaa
    18481 actatactga aactggcgga gtgattttta ttcccgtcag aaacacgcat gatcttagtg
    18541 tattcagact gaacaggaat tcaaatcttt acaaataaaa taaattttat atcaaaaggg
    18601 agaatggcat tttaaacggt tttaaatttt aattcaaaaa cagtcatcat caaatagtag
    18661 caactcattt caacttcgaa aggactctta tttagttcga aagcccgagt tgttagttcg
    18721 agatcagtca tttctatttt ggagacattt gtttttagtt cgaagaaact cgtttttagt
    18781 atgaatgatt aattgttatt tctaagacac tcaatgttag ttctaaagca tttgttttag
    18841 ttggaagaca ctcatttata ttgcgaaaac agttgtcgaa aattcgaaga catttaaata
    18901 cagtaaaatc agctatctga ataaatcgta aaatgtaaca gctatgatta acagattctt
    18961 aattaattta tatttttttt taaataaatt aattacatta aaataaaaaa tttgtatgaa
    19021 agctgacatg atttttaaaa ttaaaataat ttaaaaatgg gtgaaataaa aaatttaagt
    19081 gaatcggatg ctattgaaaa agtcaaagag ttagccgaag aaattgatat ttgtatgtta
    19141 tgtacatatg aaaacggaag aatgatggct agaccgatgt caactcagaa ggttgacgac
    19201 gaaggtaatg tttggtttct ttcggacaaa agcagtgatt caaatcgtca gatcgaaaac
    19261 aataacgaag ttgatctgct ttattccaaa gactactaca aatatttagc attgcatggc
    19321 tctgctgaaa tattattcga caaggataaa attaaagaac tctggaagaa gcatgcagaa
    19381 atctggttca ctgaaggagt tgatgatccg agaataagtg taataaaatt taattttgat
    19441 gacgggcatt actgggataa taaaaacggt cgtatagttc aactgacaaa aatggcggct
    19501 tcattactga caggaaagac aatggatgat ggcatagaag gtgatctgga aaatttatga
    19561 gcataaaata ttataaattg taatgaatct ttgcaaattt ttgtaacgtc aacacgtttg
    19621 attatttttc actgcaatgc cggaaatttt aacttacctg gttttcaact taagtgaatc
    19681 ttttaattca gattgggcat gaaatttacc acgcagaata ataccattga tattttttgt
    19741 attgttaata ttttccaatg gatcagaccc aagaagaaca aaattcgctt ttttattgat
    19801 ctcaacagtt ccaagctcat tatccatttt taaaaaaact gccggattga tcgttgccgt
    19861 ctgaagtgct tcaagaggcg tgaacccaca tttctccaca aacaatctca gttcttcatg
    19921 caggctgtaa ccgggatagc aatatggatt cgggaaatca gtaccggcta aaaatttaac
    19981 atccccatcc agcattgttc tgaaaacaga agtaacaaga gcataccagt cagattcaat
    20041 ctgtctgttt cgattttgta aatcagcttt cgaagtatca acttttggat tccagttttc
    20101 aactgcaaag tccggcataa atttaatctc ttccattggt ttgaaatccg gcagtgatct
    20161 gtttattgaa ccttcagcag caactaatgt cggacaaatc catgagttac tctgacttaa
    20221 aagagtgact aactcaggta attttgatct gtcaaaagtt tctgtttcaa atttcattct
    20281 gttaagaaaa gtagaaaact tcctgggttc aaagagtgta tcatttttct cctcattatt
    20341 ttcattaatc attgaagaat ataaagactc tttttgtgat gaacaaaatt caagaattcc
    20401 aaaaaaatgc tcaagactga tctgtccgga attcagagct tcttcaagag aaatttttaa
    20461 aggaatgtgt ccgccaaaag atatcccttg ttttttacat tcatcggcaa ttgcaaagta
    20521 aacagctctt tccaaaaaag tataaacttt aatgaaatca gctccttctg ctttttgttt
    20581 gcgcacaaat tctctacctc tttcgggaga atctgcagaa tcgctacctt tccatagcgg
    20641 tgggcttccg tctatgatcg gacccgcact aaagatgtcc ggacctttta tagatccgtt
    20701 agttattccg atccggattt tgtcaatttc ggtttgctct ccccacattt cccgaattcc
    20761 tgtcactccg tttacaagca gcatcggaaa aaaatattca taagcccacc atgagtgagt
    20821 atgcatatcc cacaatccgg ggattaagaa ctgaccctct ccgttgatca cttcgaaggc
    20881 tttataagtt gaggaattgt catgatttaa aatttcagtg attctgtctc tgataattag
    20941 tatatccatt gaccttgaga ctgatccgtc taacacattt actatgttta cattttttat
    21001 aatcagatct ccctcgagtt cttttgccga accggagata atgtgtataa aaactaagaa
    21061 gattgataat ttaaatttta ttttcataca catcagtcaa aaacgtttag cattggcagc
    21121 aaaaatataa actcgtcatt cttaaaagtg tacatttgtt ttgacacagt aacagtctct
    21181 tcgccggtct ctcttttaaa aagttcagtc ttctcaaaag taggcgtaag tatctctgaa
    21241 aaattataga tttcgaaatt ttgtcttact tttgtttcca gaaaatatct ctgacgtgct
    21301 ttttcaggaa gtagatcatt agtacctttt ggtttttgga taagcattgg caaaaatata
    21361 attttaatgt cattaaataa acacaatata caatgttcaa agctgaattt tcgattgtaa
    21421 acttttttca ggacgtgaca ccagcggttt cttcagtcat agactccgaa aaacatgggt
    21481 aaatctgacg gtaaagattt atactgcata ataaagttat caaatttttt ttgaactcct
    21541 ccacctgcgg aataatgaat tatttgattc ctacacttat actaagtatt gtttgttttt
    21601 tacattcaat aattatattt tagatcaact aaatttaata atatgaaaaa aattttattc
    21661 ctttttattc tgcttgcttt gaatataaat tcatatagtc agtggatcga atcttcaaga
    21721 ccttcacctg cttttccact tttttcgttt tcatttccgt cagctaatac aggatttgct
    21781 gtcggctatg gtaatactat gataaagtcc accaatggag gtgttaactg gtttaacatt
    21841 tcatttttta atactactgc aaatgatcta agatctgttt tttttattaa tgagaatacc
    21901 ggatggatgt gctctaccag tgatacagtt ttctatacaa caaattccgg ttcaagctgg
    21961 actccacaga tctcttttgt ttctgatgcc aatcaggttt attttactaa ctcatcgacc
    22021 ggctggatat tgtccactcc aaatcttttc agaactacaa atgcaggagt taactggaca
    22081 aaaataaaca cacagatggg atatgatttt tatttttttg gtgaaaatac aggctggagg
    22141 acattatact ccggtggcag ttcttctatt caaaaaacaa ctaacggtgg tgtaaactgg
    22201 atgactaaac acactacttc aaactttcgt gttatctatt ctatagattt tgtaaatgaa
    22261 aatacgggat gggcaggggg gtacagagaa catattttaa aaacgaccga cgccggagac
    22321 agctggattc agcaaagaga tatggataat tctgccggat tctattcaat ggattttata
    22381 aatgaaaaca cgggatgggc aatcggagat gcgggtatgt cagtttatac aataaatggc
    22441 ggctctgtat ggaatcaggt accactgaat ccgggaagag ctcagattaa atttataaat
    22501 tcaaatacag gctggatagt aggaagcaaa atttataaaa ctaatacaac cgggcttata
    22561 tataaaaatc tatcttgcaa tactttgata gaaggatttt ataatgataa atctaataca
    22621 attaattctg atagtgtaag aatttatcta agaaatggga atgcacctta ttccatgaaa
    22681 gattctgcaa tatctttaat gaattccgac ggttcaggaa catacaattt tcttaatgca
    22741 gctaacggag aaaattatta cctggtcata aaacaccgca actctttaga gacctggagc
    22801 tcttctactc aaatatttat cgcaaataat ttaaactatg atttcacagt ttcttccaca
    22861 caggcatttg gcagtaatca aatactcatt gacagctcac ctgtaagctt tgcagtttac
    22921 agcggcgatg tgaatcaaga tggtacaata gatctaagcg a
//
LOCUS       DAORVQ010000028        22402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_856_length_22402_cov_5.241375, whole genome shotgun sequence.
ACCESSION   DAORVQ010000028 DAORVQ010000000
VERSION     DAORVQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_22402_cov_5.241375"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1805
                     /locus_tag="PLX80_04060"
     CDS             171..1805
                     /locus_tag="PLX80_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20005.1"
                     /translation="MAKKESKKNVRSSAGAGTSAKYNKNVIPFDDNPLIAKYFWLVIP
                     LFTLIYFISSKYSLGFYQDDEVGQYINMLSFWSDPFVILGNSPKPGYKIFMVIPALFS
                     YSAVLIVNAFIASLAVYYTYILLKVCKINYAYFGALLLSVQPLFFDLSFRSYAEIFTS
                     LLILFVLILYKKESYSLSALLCGYIFTVRQEVAVIILIFAVIYFRKKVYLPIILLAVF
                     PLIYDLLGFLKTGDIMFVLTEIMSLSVYSYKTQGISHYLLVYIFIVGPVTLLLFFFGF
                     FGFLSDTDKIKEYLMKYGIFYLIFTSIFFVQLMTMFSDGANPGNWRYLLHISPIAALF
                     ATVGLNNLAMPSFKKSSYILTGILAFITLAFLSKDTNGFILLETSEYSKFFVIAISFL
                     IILLIKKEPAVEYLNKVSAVLLMIAIVYLMISFTPKVLSTENVALKETSEYLNTLDLK
                     DKEILYNHTFIPFYSDELYRESPEKFKRLILENLKDIKPGTIIVWDSHYSYRPKDMLN
                     DVQLETLEKDSTYKLLNKIVSSDQRFGAFIFEKSNQ"
     gene            1815..2768
                     /locus_tag="PLX80_04065"
     CDS             1815..2768
                     /locus_tag="PLX80_04065"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY20006.1"
                     /translation="MNSDKKKQRCIIYGGGGFIGSHLCEKLLELGYDVTVFDKLNFSK
                     KNISHILDRINVIEGDFNNEFDLMNSLKGIDYVYHLVSSTLPASSNDNPEYDAETNLI
                     SSLKLFKECVNLKIKKIVFLSSGGTVYGIPDEIPVNENYYSNPVCSYGIIKRTIEEYL
                     YLFNFLYGQKYFVFRLSNPYGERQNPNAAQGAIAVFLNKVINDQKIEIWGDGEVVRDY
                     IYIKDAVEVLAGSLKIEADEKVFNLSYGQGYSLNQIIETIKNITDKKINLEYQKSRKL
                     DVPVNILNNEKVKKTFNWSPRTNLEDGIKKTFEHFSEEKNL"
     gene            2765..3499
                     /locus_tag="PLX80_04070"
     CDS             2765..3499
                     /locus_tag="PLX80_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY20007.1"
                     /translation="MTDNSDISLSVFFPAYYDEKNIGKVVDAAVIVLEELKLKDYEII
                     IIEDGSPDKTDEVADALAEKYEKVRVIHHEKNMGYGATLKDGFMSAKLDYVFYTDGDN
                     QFDLDELKKFVALLPFTDIVVGYRKHKQYSLYRKFTSLCYNYLLKLVFDIHYWDIDCA
                     FKLFKTDLFRKIKIDSVDAFIDAEIMLKANLLDYSVTEVGVMHLPRVDGISTGARPSV
                     IIGTIKEVFSYRKEYFSELAKKTTSS"
     gene            complement(3486..4202)
                     /locus_tag="PLX80_04075"
     CDS             complement(3486..4202)
                     /locus_tag="PLX80_04075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20008.1"
                     /translation="MNIENKINDVPGKVDFDNYAGRYEKVLDKQLDFFGEENSYFADY
                     KVKLVKDTLTSDPENILEFGCGIGRNLKFFTKYFPDADIYGSDISDKSLEIAKNENPS
                     VKFFMLEDDEIKNYKGKFDLIFISCVLHHIAPDLRKSSMDTVYSMLNNNGSAYIFEHN
                     PYNPVTRHIVNTCEWDTDAVLLNMKESVNLFENAGLKVTEKNYTLFYPAALKFLRFTE
                     NFLKKIPMGGQYYVKAVKKK"
     gene            complement(4206..6497)
                     /locus_tag="PLX80_04080"
     CDS             complement(4206..6497)
                     /locus_tag="PLX80_04080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQY20009.1"
                     /translation="MSDKEKFNLSTLTKLPNQKKSSQEELFEFEVIGMDCTSCAKSIK
                     TYMENVDGVFKVEINYASESGEIIFDPSLLTKEKIVKDIKKLGYDVSGDEEGYLSEQL
                     KIKNLKNQRNKIFVSVSLSLLIMILSMRDHFGISFISDISEKTLLLILTLLSSVVIFW
                     CGSKFISGALISLKNLSFDMNTLISMGSLSAYFYSLVITLNIVFDLKLTSLASSHEVY
                     FETGAMIITFILIGNYLESVMKSKTQTSVQKLKDLQAKVVNVIRDGNEMFIPFKKVKL
                     HDIVLIKTGDKIPVDGKIIEGFCVVDESAMTGESLPVEKRAGDDLMSGTVLRNGFVKM
                     DALKVGKDTMLSKIIALVKDASSNKPKIQKLADKISSVFVPIVIVIAALTFSYWFYIA
                     DAAFDRSLLFAVSVLIIACPCALGLASPIAIVIGVGRAAENGILFSNVDAIENLMKID
                     TVCFDKTGTLTTGEMSVKNVYTRNGYTEYDLMKYIYSVEKLSTHPIAKSINNYSVDNS
                     VLTFNNVTDFKNESGFGVSANVNNKRVMIGNEELFRKNNITFPEIFYNSGYTNLFVSI
                     DENISGIIEIEDKIKDEAKSVIEKLNKKNFDLFMISGDSENAAAKTAGELGIKNYSFK
                     TLPEEKEKIISNLQKKNKNVIMIGDGINDAPSLARANVGMAIGTGQDIAIDSADVILV
                     KDDLRNILKSIVISTKTVSIIKQNFFWAFFYNAIAIPLAAGLFVPYGIFISPVMAAMI
                     MAFSDVVTVIGNSLRLKYVNIDQ"
     gene            complement(6514..7350)
                     /gene="folP"
                     /locus_tag="PLX80_04085"
     CDS             complement(6514..7350)
                     /gene="folP"
                     /locus_tag="PLX80_04085"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQY20010.1"
                     /translation="MTCDLSERTYVMGILNITPDSFSDGGKYYDGKLHLEKVIEDAVR
                     MEKEGADFIDVGGESTRPGAESISIDEEITRVVPVINAICKKINIPVSIDTYKSEVAE
                     EALKEGACIVNDISGFRFDEKLPEVTAKYNASCILMHIKGTPKNMQQDPEYDDVIKEI
                     SEYLETSADIAKDAGIKQIILDPGIGFGKSFENNLEIIRNLSEFKKLGYPILLGTSRK
                     SFLNKIFSSTPEKRLEGTIASNVIGIMNGADIIRVHDVAENFKAAKTTDTIINKIPAL
                     KL"
     gene            complement(7368..9416)
                     /gene="metG"
                     /locus_tag="PLX80_04090"
     CDS             complement(7368..9416)
                     /gene="metG"
                     /locus_tag="PLX80_04090"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQY20011.1"
                     /translation="MKKNKKYLVTAALPYANNYLHIGHIAGAYLPPDIFVRFKKLNDE
                     DVIFISGSDEHGTAIEMASISENVSPQEIIDRYHFANEKAFQDLGINFDIYSRTSNKV
                     HHETAKEFFLNLYSKGLFVQKTEKQLFSEKENRFLADRFITGTCPVCGFEEARGDECE
                     NCGSNLSPLELINPRSKITGDIPVVKESTHFYFPLGDYQDALNNWLEDKKYWKQNVIN
                     YCKGWLKAGLRDRAITRDLDWGVKVPVEGNDGKVIYVWFEAPIGYISATKELFALRNE
                     PEKWKDYWQNKDTKLIHFIGKDNIVFHAIIFPSMLMAHGDYILPDNVPGNEFLNISGA
                     KLSKSKGNGILVKDIVKIFSPDVIRYVLASIMPENKDSEFTWKDLQIKNNSELAGILG
                     NFINRTVVFAKNKFDNKIPELHKIENADEEIFNYFKSQSEKVSELYDKFSFREALLNT
                     MDCVRGANKYFNDNEPWKSVRDDKERCETVIRVCLELCHSISILINPIIPFTSESILN
                     ILNVSNKDLKWDKIGKCVLQTGAELGDNKILFPQIEDSEIEKLEADSSVEKKEKDVKN
                     AVKSDKKAAGVITLEDFKKVSLKVAKIVECEAVPKSKKLLKLKLKVGDIEKQIVSGIS
                     EHYKPEDLIGKLIVVVDNLVPSKLMGVDSEGMLLAAKQNGELKIITIDGDIESGADVN
                     "
     gene            complement(9489..9893)
                     /locus_tag="PLX80_04095"
     CDS             complement(9489..9893)
                     /locus_tag="PLX80_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HQY20012.1"
                     /translation="MLDKFKIILFDGVCNLCNSSVNFIIDNDHKNVFKFTSLQSEKGI
                     ELLKKYDLLDKNIDSVILVENDKAYVKSDAALKIAKTLGGKYKLFYGLIILPAFIRNF
                     FYDIIAKNRYKWFGKKDSCRIPTLELKSKFLE"
     gene            9961..10656
                     /locus_tag="PLX80_04100"
     CDS             9961..10656
                     /locus_tag="PLX80_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY20013.1"
                     /translation="MSKNTVAVIGDVHGCYYTLKKIHDKLKNAGEVYTVGDLIDRGKY
                     SKAVVEYCISNSIKSVRGNHEDMMLKAIEKSDKFLGFMFKEAEHYYYNGGKETQNSYI
                     DSKAFSDFKKFKQKLKDLGHYDFIQSFPLKYEFKKVVISHAGIIPGGDDVSILWNRRT
                     PEKLDKLQIFGHTPLREYVYKKEYYSNIDTGCVFNNKLTAVIVDINEGIIVDVLDENC
                     DENDVDPEAEMEL"
     gene            10745..11020
                     /locus_tag="PLX80_04105"
     CDS             10745..11020
                     /locus_tag="PLX80_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HQY20014.1"
                     /translation="MENIHVKNAEYLEEYKINVEFNTGLNQVIDLKDEIWEEIFEPLK
                     DKEYFRNFKLNPFTIYWENGADFSPEYLFEMAKKQEFMIADKKRNQN"
     gene            11291..11773
                     /locus_tag="PLX80_04110"
     CDS             11291..11773
                     /locus_tag="PLX80_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY20015.1"
                     /translation="MKIHNPVRAYLRDMDSFFYGGNGWQAGLFEQVKDLNFKQAVWRP
                     DINRNSIWKIVKHTIFWKQYIFSEEEGKPFSSEERRSGNWMSIPEKPDEEKWQSELLL
                     LEMTHKKMKSLIRKKDIELFNTRKEFSNYIREIISHDAYHAGQIGLVRVLQGIKPAKY
                     "
     gene            11881..14340
                     /gene="gyrA"
                     /locus_tag="PLX80_04115"
     CDS             11881..14340
                     /gene="gyrA"
                     /locus_tag="PLX80_04115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQY20016.1"
                     /translation="MPNEKIIPINIEDEMKSSYIDYSMSVIVSRALPDVRDGLKPVHR
                     RVLFGMRELGLASNRAHKKSARIVGEVLGKYHPHGDKAVYDTMVRLVQEFSLRYPLVN
                     GQGNFGSVDGDSAAAMRYTEARLSRVAEAILEDLEKDTVDFVPNFDDTLTEPSVLPAV
                     IPNLLINGSNGIAVGMATNIPPHNLNEICNGIIQLIKDPEITIKKLMKFVTAPDFPTG
                     GIIYGYQPVIDAYETGRGKIILRAKVSIEVEKNGKHNIIISEIPYQVNKVSLIENIAQ
                     LVRDKKVEDISDINDESDRDGMRVVIGLKRDGNPQVIVNNLYKHTQMQTTFGTIMLAL
                     VDGIPKVLNLKEMMEHFIKHRLNVIVRRTKFELDAAEKRAHILEGYIIALDNIDEIIK
                     LIKKSPDAPTARTALMKSFKLSEIQATAILEMRLQRLTGLERKKIEDEYKEVLMTIER
                     LKRILDNEQLRKDMICDDLKALVEKFGDERRTEIVYDVRSMTDDEMMKELVKEEDVVI
                     TISNKGFIKRIPVTSYKSQGRGGKGITAASMGSAEDDFNEHMFIGSTHQYIMFFTKLG
                     KCYWLKVYDIPEGSRTSRGKSILNLIEKDKDDDISSFVIVKDFTEELFVTMVTRSGVI
                     KKTKLDSYSNIRKSGINAINLKDDDTLVDVKLTNGTQEIVIGSHNGQAIRFNETKARE
                     MGRTATGVKGIKLAKDDYVIGFVTVSRPSATILVITENGYGKRSNVGDYRVTNRGGKG
                     VITVKTTDKVGKLISIKEVTDTDDLMIITSKGILIRQNVKSIRVMGRNAQGVRVIRLN
                     DGDTISAVARIVEDEKVKPVE"
     gene            14361..15386
                     /gene="ltaE"
                     /locus_tag="PLX80_04120"
     CDS             14361..15386
                     /gene="ltaE"
                     /locus_tag="PLX80_04120"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HQY20017.1"
                     /translation="MSKIIDIRSDTVTKPSPAMLEAIMNAKLGDDVFGEDESVNELEK
                     KCADLSGKESAIFVPTGVMGNQLAIKCHTDPGDEVIVESESHILNYETAAPSVISNVQ
                     LLPVPGKNGVITAEDIEKYIRTNEYYFPVTKLICLENTHNRSGGVIQPIDVIKGISDL
                     AKRYGIRTHLDGARIFNAVVETGISLKEYADNFDSISFCFSKGLGCPVGSILCGSNEL
                     IDKARKWRKILGGGMRQAGILAAAGIYALDNNIERLKEDHSKAKYFAKELSKLESVSI
                     DMSTVMTNIVIFGSDKYSKKEIIDKAKNNGVIISSGSYGLMRAVFHLDVSMDETKKAI
                     DKLKTIL"
     gene            15469..15966
                     /locus_tag="PLX80_04125"
     CDS             15469..15966
                     /locus_tag="PLX80_04125"
                     /inference="COORDINATES: protein motif:HMM:NF023737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar dehydrogenase complex small subunit"
                     /protein_id="HQY20018.1"
                     /translation="MISRRDFIRFCSVAAVGASIIPLIEGCTEKNIEFGNTEFMKLSS
                     VLTGFSETELDKTLAAAYQTSLTDFPPAKTTLKELYISLNIDSNKIPSDEEIENVITK
                     NPEYKKLADTLITYWMSGIYKTKDGMKVSDYEKMYAWKSTGYLIPNAQCRGDFGFWQN
                     KPVVT"
     gene            15985..17703
                     /locus_tag="PLX80_04130"
     CDS             15985..17703
                     /locus_tag="PLX80_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQY20019.1"
                     /translation="MQKSDYSADVVIVGAGISGALMALGLAKANAKVLIIEAGDRIDR
                     SKAIEKYKADPERGDDSPYNPVSRTAPYPPMDDPQQYYIQMGPDKFQSTYIKAVGGTT
                     WHWLGTCLRFVENDFKMKTEYGVGTDWPITYKEIEKWYFAAEQEIPVSGDSNAALGVK
                     REKPYPSAMIPQTYLDKQVDIAFQNLNLPDATDIKLQVASTPQGRLSAPQDERLACCG
                     SNNCIPICPVGAKYDASFTIAKAEALGVKVLTNSTAKFIQTDDEGSVTGILFQTADGD
                     KIAAGKIFILAGHAIETPKLMLMSKTEKYPNGIANSSDQVGRNLMDHNCVLSYGLTKD
                     PVYPYQAPLSTSGVENVKDGPFRKERGAFRIEIGNDGWSWPVGYPNVLSSYFLKKSGG
                     RSSVMNLPVTDEVIKQGQNNLLYGKKLRNDIKENVLREIRFAFLCEQLPDPNNRIVPA
                     FDSLGTDGLPRPRIFYKLDDYVKASIDVAVTLQKSMLDSLGCTQQHHSYPWGAGHIVG
                     TTKMGDDPKTSVVDKNLKCHDLKNMYILGSSVFPTVAASNPTLTIAALTLRAVEEIQK
                     KLLELN"
     gene            complement(17717..18796)
                     /locus_tag="PLX80_04135"
     CDS             complement(17717..18796)
                     /locus_tag="PLX80_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQY20020.1"
                     /translation="MNKTYFTPGPTELYPNAKKDLSDALDKKIFSLNHRSIEFMEIFK
                     NTVSSLKTLMNIPENYHVFFLSSATECMDRIIQNCTVKNTFHFANGAFADRFYKTAVE
                     LGRNAVKSEVDYGSSFNFDDIKIDNDPEVICITQNETSTGVAIDVQNIYDLKIKYPDS
                     IIAVDIVTSAPYVNIDFSKIDCAFFSVQKGFGLPAGLGVLILNDRCLQKAEKLKSDGI
                     KIGSYHNFISLLENELMYQTTETPNMPGIYLLGKVCEQLNEKGISEIRKETEEKSDML
                     YNFFNTHRSFTLFVKNTENRSKTIIVIETGSRQVEVKKLLSDNGIIVGSGYGKFKTEQ
                     IRIANFPMHKKEDVLKIINTLKHLD"
     gene            complement(18809..20704)
                     /gene="serA"
                     /locus_tag="PLX80_04140"
     CDS             complement(18809..20704)
                     /gene="serA"
                     /locus_tag="PLX80_04140"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQY20021.1"
                     /translation="MNKSKIKYIIDFDSTFIKLEALDELFNISNIKGIKSDLELKEFE
                     KITSSSMNGSLSFSESLKKRMKLLKADRTHIDKLVKKLSGKVSSSVKRNKSFFINHSE
                     NIYIISVGFKELIVPVVGKFGIPAENVFANTFTYDKDGNISGYDKKNLLSFDNGKIEQ
                     LKQLGLSGELYVIGDGYTDYELKKAGIAHKFFAFTENVEREIVSSKADHITPSMDEFL
                     YLNKLPMTISYPKNRIKVLLLENIHKDAVSIFKEEGYQIESFPKSMDEKELCKKIKDV
                     SILGIRSKTQISRQVIDHADKLISIGAFCIGTNQIDLSTALKKGIAVFNAPFSNTRSV
                     VELALAEIIILMRRIFDKSTSLHKGIWDKSAAGSFEIRGKKIGIIGYGKIGSQLSVLA
                     EDLGMDVYYYDVVDKLALGNATKCNSLNELLKKCEIITLHVDGKDSNTNFIGEKEFKG
                     MRDGTVFLNLSRGHVVDIEALAKCLISGKISGASVDVYPEEPSGNDEAFVSVLQNIPN
                     VLLTPHIGGSTEEAQRNIADFVPKKIIEFINSGNTFYSVNFPEIQLPQFQNAHRLIHI
                     HENVPGILAQINNILASHNINIIGQYLKTNELVGYVITDIKKKYSPQVIQDLKKIPHT
                     IKFRILY"
     gene            complement(20916..20987)
                     /locus_tag="PLX80_04145"
     tRNA            complement(20916..20987)
                     /locus_tag="PLX80_04145"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20953..20955),aa:Gly,seq:ccc)
     gene            complement(20994..21494)
                     /locus_tag="PLX80_04150"
     CDS             complement(20994..21494)
                     /locus_tag="PLX80_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQY20022.1"
                     /translation="MITIILTFVLIFFTSIISLAGDISPDQKYSKSRIKSLAKEYVNN
                     NLNINNPVMVDIDEDGDFDILKFNDGKVEYYKNTGSLDKPQFVLENKNFDNYEMRSIF
                     PVSIPFPVFMADRDGDLDKDIFGIVERNNKSEIVYAENTMDLDHYTLITIILVLLIVV
                     LIIAIV"
     gene            21656..22111
                     /locus_tag="PLX80_04155"
     CDS             21656..22111
                     /locus_tag="PLX80_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20023.1"
                     /translation="MPKIILIFITALVLFGCSQKNVHDKQTSINEIKKADKEMSALAE
                     QEGFSTALLKNADENFVKLSEGIHPVIGKSAFEELTNGKPGPKTLTWEAVFTDVAESG
                     DLGYSWGNWKFVLPDTVFYGNYFTVWKKYNDGTWKMTLDGGNGTPKPND"
BASE COUNT         7512 a         3734 c         3831 g         7325 t
ORIGIN      
        1 ttgaaatttg aaagttgtaa gataaatttt ttgaagttca aattctttgt aaaaaacggg
       61 tagagaattt aagtcaatca tttttgaatt agatttgaaa aaatctatcc attctccttt
      121 tcaaagtaat ttcaattgcg gatttatatt aatatatttg aaacttattt atggcaaaaa
      181 aagaatcgaa aaaaaatgtg cggagctctg caggtgcagg gacatctgct aagtataata
      241 agaatgtaat tccatttgat gacaatccat taattgcaaa atatttctgg ttagtcatac
      301 cgctgtttac attgatctat tttatcagca gtaaatattc actgggattt tatcaggatg
      361 atgaagtagg gcagtatatt aatatgcttt cattctggag cgatccattt gttatacttg
      421 gaaattcccc aaagccgggt tataagatct ttatggtcat tcctgctcta ttcagttata
      481 gtgcagtcct gatcgtcaat gcattcatag cttcgctggc agtatattac acttacattt
      541 tattaaaagt ctgtaaaatt aattatgcat atttcggtgc gctgctttta tcagtgcagc
      601 cgttattttt tgatctgtcg ttcaggtcat atgctgagat tttcacgagt ctgttgatat
      661 tgttcgttct tatactttat aaaaaagaaa gctattcttt gtctgcactt ttatgcggtt
      721 acatttttac tgtcagacag gaagtagctg tgatcatact aatttttgct gtgatctatt
      781 tcagaaagaa agtttatctg ccgataattt tacttgcagt atttccactg atatatgatc
      841 tgctcggatt tttaaaaacc ggtgatataa tgtttgtact aacggagatc atgtcgcttt
      901 cggtatatag ttataagact cagggaattt cacattattt acttgtatat attttcattg
      961 tcggacctgt tacattgctg ctgtttttct ttggcttttt tggattccta tctgatactg
     1021 acaagataaa agaatattta atgaagtatg ggattttcta tttaatattt acttcaatat
     1081 tttttgttca gcttatgaca atgttcagcg atggagctaa tccgggcaac tggcggtatc
     1141 tgctgcacat atcacccata gctgcgttat tcgctactgt aggtctgaat aatcttgcaa
     1201 tgccttcatt taaaaagtca tcttatattc tgacaggaat actggcattt attactcttg
     1261 catttttatc taaagataca aacggtttta ttttactcga gacttctgag tattctaaat
     1321 tctttgtaat agctatttca tttttaataa tattacttat aaaaaaagaa cctgctgttg
     1381 aatatctgaa taaagtatct gcagttttat taatgatcgc aattgtttat ctgatgataa
     1441 gctttactcc aaaagtttta tcaacagaga atgtagcttt gaaagagact tcagaatatc
     1501 tcaatactct tgatctgaag gataaagaaa ttttatataa tcatactttc attccatttt
     1561 acagtgatga gctttacagg gaatctcctg aaaaattcaa aagacttata cttgaaaatc
     1621 tgaaagatat aaagccgggt accattattg tctgggattc gcattatagt tacagaccaa
     1681 aggacatgct gaatgatgtg cagctggaga ctcttgaaaa ggacagcact tacaaacttc
     1741 tcaataaaat agtatcatct gatcagagat ttggagcatt catttttgaa aaatctaatc
     1801 agtaaatttc ggatatgaat tcagataaga aaaagcaaag atgcataatt tatggaggag
     1861 gcggattcat cggatctcat ctttgtgaaa agcttcttga gcttggatat gacgtaactg
     1921 tttttgataa gcttaatttt tcaaaaaaaa atatttcaca tatactcgac aggataaatg
     1981 taattgaagg tgattttaat aatgaatttg atctgatgaa ttcccttaag ggaattgatt
     2041 atgtctatca ccttgtgagc tctactcttc ctgcatcatc caatgataat cccgagtatg
     2101 acgccgagac aaatcttata tcgtctttaa aactttttaa agaatgtgta aatcttaaaa
     2161 taaaaaaaat agttttcctt tcttcaggtg gaacagttta tggtataccg gatgaaattc
     2221 ctgtaaatga aaattattat tcaaatcctg tttgttcata tggcattata aaaagaacta
     2281 ttgaagaata tttatatctg tttaattttc tttacggaca gaagtatttt gtatttcgtc
     2341 tttcaaaccc atatggtgag agacagaatc caaatgcagc acagggagca attgcagtat
     2401 ttctcaataa ggtgatcaat gatcaaaaaa tagaaatatg gggtgacggc gaagtggtaa
     2461 gagattatat ttatattaaa gacgcagtgg aagtactggc tggatcgtta aagattgagg
     2521 cagatgagaa agtatttaat ctaagctacg gacagggcta ttcactgaat cagatcattg
     2581 aaacaataaa aaatattact gataaaaaaa taaatttaga atatcagaag tcaagaaaac
     2641 tggacgtacc agtaaatatt cttaataatg aaaaagttaa gaaaacattt aactggtcac
     2701 cccggacaaa tcttgaagac ggaattaaaa aaacatttga acatttctca gaggaaaaaa
     2761 atttatgaca gataattctg atatttcatt atcggtattt ttccctgcat attatgatga
     2821 gaaaaatatc ggaaaggtcg tagatgccgc tgtcatagtg cttgaagaat taaagctgaa
     2881 ggattatgag atcataatta ttgaagatgg aagtccggat aagactgatg aagtagcaga
     2941 tgcacttgca gaaaaatatg agaaagtgag ggtcattcat catgagaaaa atatgggata
     3001 cggtgcaact cttaaggacg gatttatgag tgcaaaactt gattatgtgt tttatactga
     3061 cggtgataat cagttcgatc tggatgaact gaaaaaattt gtagccctcc ttccatttac
     3121 agatattgta gtcggttaca gaaagcacaa gcagtattct ctttacagaa aattcacttc
     3181 tctatgctat aactatttac taaaactggt ttttgatatt cattactggg acattgactg
     3241 tgcatttaaa ttatttaaaa ctgatctttt cagaaaaatc aaaatagatt cagtcgatgc
     3301 attcatcgat gcggaaataa tgctgaaggc aaatctactt gattatagtg taacggaagt
     3361 cggtgtaatg catcttccga gagttgacgg aatttctaca ggagcaagac cttcggtaat
     3421 aatcggaaca ataaaggaag tgttttcata cagaaaggaa tacttcagcg agctggcaaa
     3481 aaagactact tcttcttaac tgcttttaca taatactgac cacccatcgg gatcttcttt
     3541 aagaaatttt cagtgaatct caaaaactta agcgcagcag gataaaacaa tgtgtaattt
     3601 ttttctgtta ctttgagtcc tgcattttca aataaattca cagactcttt catatttaat
     3661 aaaactgcat cagtatccca ttcacacgta ttcacaatgt gacgagtgac cggattgtat
     3721 ggattgtgct cgaaaatata tgcacttccg ttattattca gcatagaata tacagtatcc
     3781 atcgaacttt ttctaaggtc cggagctatg tgatgcagaa cacatgatat aaagatcaga
     3841 tcaaattttc ctttgtaatt ttttatctca tcatcttcca gcataaaaaa tttaactgaa
     3901 ggattttcat tttttgcaat ctcaagactt ttatcagaaa tatcagatcc ataaatatca
     3961 gcatctggaa aatattttgt aaaaaatttc aaattcctac caataccgca tccgaattca
     4021 agaatatttt cagggtcaga agtcaatgta tcttttacca gttttacttt atagtctgca
     4081 aaataagaat tctcctcacc aaaaaaatcc aattgcttat ccagaacttt ttcatatctg
     4141 ccggcataat tatcgaaatc aactttcccg ggtacatcat ttattttatt ttcaatattc
     4201 atttatcatt ggtctatgtt tacatattta agtctcagtg aatttcctat cacagtaacc
     4261 acatcactaa aagccataat catagctgcc atgacagggc tgataaaaat tccgtatggc
     4321 acaaaaagtc ctgctgcaag aggaatagct attgcattat agaaaaatgc ccagaagaaa
     4381 ttctgtttaa ttatgcttac agtcttagtt gagataacta ttgatttcaa aatatttctc
     4441 agatcatcct ttactaaaat tacatctgca gaatcgattg caatatcctg ccctgtacca
     4501 attgccattc ctacatttgc tctagcaaga gagggagcat cgttgatccc gtcaccgatc
     4561 ataattacat ttttgttttt cttctgaaga tttgaaatta ttttttcttt ctcttcagga
     4621 agagtcttga atgaatagtt tttaattccc agttcgccag ctgtttttgc tgcagcgttt
     4681 tcactgtctc ctgagatcat aaataagtca aagttctttt tatttaattt ttctataaca
     4741 ctcttggctt catcttttat tttatcttct atttcaatta tacctgaaat attttcatct
     4801 atacttacaa agagatttgt ataacctgag ttataaaata tttcgggaaa ggtaatatta
     4861 ttttttctga ataactcctc attaccgatc attactcttt tattgtttac attagcactg
     4921 accccaaatc ctgattcatt tttaaaatca gtaacattat taaatgtcaa aacgctatta
     4981 tcaacagaat aattatttat agactttgca atcggatgcg ttgaaagttt ttccactgaa
     5041 taaatatatt tcatcaggtc atattccgta tatccattcc ttgtataaac atttttcaca
     5101 gacatttcac cggtagtcag tgttcctgtt ttatcaaaac atacagtatc gatcttcatc
     5161 agattttcta ttgcatctac attgctgaaa agaattccat tctcagcagc tctgccaact
     5221 ccaatcacaa tcgcaatagg ggatgcaagt ccgagagcgc agggacatgc aatgatcaga
     5281 actgacacag caaacaaaag tgatctgtcg aaagcagcat ctgcgatata aaaccagtat
     5341 gaaaatgtta atgcagcaat gacaataacg ataggaacaa atacagatga gatcttgtct
     5401 gcaagtttct ggatcttcgg tttattcgat gatgcatctt ttacaagtgc tattatctta
     5461 gataacatag tatccttgcc gactttcaat gcatccattt ttacaaatcc atttctcaat
     5521 actgtaccgc tcatcagatc atcgcctgct cttttctcaa ccggcagact ttctccggtc
     5581 atagcacttt catcgacgac acaaaatcct tcaatgatct tcccgtcaac cggaatcttg
     5641 tcgccggttt taataagtac tatgtcatga agttttactt ttttaaatgg aatgaacatt
     5701 tcatttccgt ctcttataac attcacaact tttgcctgaa gatctttaag cttctgcacg
     5761 gaagtctgtg tcttcgattt cattacagac tcgagataat tacctatcag aataaatgta
     5821 atgatcattg caccggtttc aaaataaact tcatgcgatg aagcaagtga tgttagctta
     5881 agatcgaaca ctatattaag agtaatgacc agactgtaga aatatgctga cagacttccc
     5941 attgaaataa gtgtattcat atcaaaagaa agattcttta atgagatcaa tgctcctgag
     6001 atgaatttcg aaccacacca aaaaataact acagatgata ataaagtaag tattaacagt
     6061 aacgtctttt ctgatatgtc agaaataaaa gaaattccaa aatgatcacg catgctgagt
     6121 atcattatta agagtgataa cgatacagat acaaatattt tgtttctttg attttttaga
     6181 ttttttattt ttagttgttc ggataaatat ccttcttcat cacccgatac atcataacca
     6241 agttttttaa tatccttaac gatcttttct ttagtgagca gtgaaggatc aaaaattatt
     6301 tcgccgcttt cggaagcata attgatttct actttgaaaa ccccatctac attttccatg
     6361 taagtcttga tactttttgc acagcttgta caatccattc ctattacttc aaattcaaaa
     6421 agctcttcct gtgaagattt tttttgattg ggaagtttag ttaaagtgct tagattaaat
     6481 ttctctttgt cactcattgt ttttaatttt tacttataat tttaatgctg gtattttatt
     6541 tattattgtg tcagtagttt ttgctgcttt gaaattttca gcaacatcat gtactcttat
     6601 tatgtctgca ccattcatta tgcctatcac gtttgaagct atcgttcctt ccaatctttt
     6661 ttcaggggtt gatgaaaata ttttatttaa aaaacttttt cttgaagtac ccagaagtat
     6721 tggataacca agctttttaa attcagacaa attcctgatg atctcaagat tattttcaaa
     6781 tgatttccca aacccaatac ccggatctaa tattatctgt ttaatgcctg catcttttgc
     6841 aatatccgcc gatgtctcca ggtattctga aatttctttt atgacatcat catattccgg
     6901 atcttgctgc atatttttag gtgtaccttt tatatgcatc aatatacagg aagcattata
     6961 ctttgcagtt acttcaggaa gtttttcatc aaatctgaaa ccactaatat catttactat
     7021 acaagctcct tcttttaatg cttcttctgc aacctcactt ttatatgtgt ctattgaaac
     7081 aggaatattt atttttttac aaattgcatt tattaccgga accactcttg ttatttcctc
     7141 atcaatactt attgattccg cacctggtct tgtagattca ccaccgacat caataaaatc
     7201 cgctccttcc ttttccattc ttactgcatc ttctatgact ttttctaaat gaagttttcc
     7261 gtcataatat tttcctccgt cagagaatga gtcaggagtt atgttcagaa ttcccatgac
     7321 ataagttctt tctgaaagat cgcaggtcat attattaaac ctgtaaatca atttacatct
     7381 gctccagatt ctatatcacc gtcaatagtt ataattttca actcaccatt ttgtttcgcc
     7441 gcaagaagca ttccttcgga gtcaactccc ataagtttag agggaacaag attatcaacg
     7501 actacaatta atttcccgat aaggtcttca ggtttataat gctcggaaat acctgaaaca
     7561 atttgtttct caatatctcc gactttaagt ttcagcttta acaatttttt acttttggga
     7621 acagcttcac actcaactat ctttgcaact ttgagactta cttttttaaa atcctctaaa
     7681 gtgataacac ctgctgcctt tttatcagat tttacagcat tcttcacatc tttctctttt
     7741 ttctcaactg aagaatctgc ttccagtttt tctatttcag aatcttcgat ctgtgggaag
     7801 agtattttat tgtcgccaag ctcagcaccg gtttgaagaa cacatttgcc aatcttatcc
     7861 cacttcagat ctttgttact tacattcaga atattcaaaa tactttcgga tgtaaatgga
     7921 atgatgggat ttatcagaat tgaaatcgaa tgacaaagct caaggcaaac tcttattaca
     7981 gtctcgcatc tttctttatc atctcttaca gatttccatg gttcgttatc attaaaatat
     8041 ttatttgcac cacggacaca gtccatagta tttaataatg cttctctgaa actaaatttg
     8101 tcataaagtt cgctaacttt ttctgactga gatttaaaat aattaaaaat ctcttcatct
     8161 gcattttcga tcttatgaag ttcaggtatt ttattatcaa atttattttt tgcaaacact
     8221 acagttcggt ttatgaaatt tccaagtatt cccgccagtt cgctgttatt ttttatctgc
     8281 agatctttcc atgtaaactc tgaatcctta ttttcaggca taatagaagc aagtacatac
     8341 cggataacat cgggactgaa aattttcacg atgtctttaa caagtattcc gttgccttta
     8401 ctttttgata gtttggcacc actaatattc aagaactcat ttcccggtac attatccgga
     8461 agaatgtaat caccatgtgc cattaacatc gaagggaaaa taatagcgtg aaatactata
     8521 ttatcttttc caatgaaatg aattaatttt gtatccttat tctgccagta atctttccat
     8581 ttctccggct cgtttcttaa tgcaaaaagt tcttttgttg ctgaaatata accgatcggt
     8641 gcttcaaacc aaacgtatat gacctttccg tcattacctt cgacaggaac ttttacaccc
     8701 cagtcaagat ctctggttat tgctctgtca cgtaaacctg ctttgagcca gcctttacag
     8761 taatttatta cattctgctt ccagtatttt ttatcttcca gccaattatt tagtgcatcc
     8821 tgataatctc caagaggaaa ataaaaatga gtggattctt ttactacagg aatatcaccg
     8881 gtaattttag atcttggatt gatcaattca agaggggaaa gattgctacc gcaattttca
     8941 cattcatctc cgcgagcttc ttcgaatcca cataccggac atgtacctgt aataaatctg
     9001 tctgctaaaa atctgttttc cttttccgaa aaaagttgtt tctcagtttt ctgaacgaag
     9061 agacctttgc tatataaatt aagaaaaaat tcttttgcag tttcgtgatg aactttattt
     9121 gaagtacgcg aataaatatc aaaattaatt ccaagatcct ggaatgcttt ttcatttgca
     9181 aaatgatatc tgtcaattat ttcctgcgga cttacattct cagaaataga agccatctca
     9241 atagcagtac cgtgttcatc agatcccgaa ataaatatca cgtcctcatc atttagtttc
     9301 ttaaatcgca caaatatatc cggaggaaga tatgctccgg caatatgtcc aatgtgcaga
     9361 taattattgg cataaggaag tgctgccgtt acaagatatt ttttattttt tttcatcaat
     9421 caatatactt aatcactgtt tttttataaa gggcatagaa ttttctacac cttactcaat
     9481 ctaaattact attccaaaaa tttactttta agctcgagag ttggaattct gcaggagtcc
     9541 ttttttccaa accatttata tctgttcttt gcaataatgt cataaaaaaa atttctaata
     9601 aaagccggta aaattattaa gccataaaac aatttatatt tacctccgag cgtctttgca
     9661 atttttaatg cagcatctga tttaacataa gctttgtcat tttcaactaa gataacagag
     9721 tctatatttt tatcaagaag atcgtatttt tttaaaagct ctataccttt ttctgactgt
     9781 agtgatgtaa atttaaatac atttttatgg tcattatcaa ttatgaaatt tacagaagaa
     9841 ttacataaat tacagactcc gtcaaaaaga attattttaa atttatccaa aattttaatt
     9901 taataagttt aatttaatgc aaataattca taaattttat gcaaacccaa atcacatttt
     9961 atgtcaaaaa atactgttgc cgttataggt gatgtacacg gctgttatta tacattgaaa
    10021 aaaattcatg acaagctgaa aaatgcaggt gaagtttata ctgttggaga tctgatcgac
    10081 agaggaaaat attctaaagc tgttgttgaa tattgtatat ccaattctat aaaatctgta
    10141 aggggtaatc atgaagacat gatgctgaag gcaattgaaa aatcggataa atttcttggt
    10201 tttatgttta aagaagcaga acattattac tataacggag gaaaagaaac tcaaaattct
    10261 tatattgatt caaaagcatt ttcagatttt aaaaaattta aacaaaaatt aaaggatctc
    10321 ggtcattatg atttcattca aagttttcct ttaaaatatg aatttaaaaa agtagtaatt
    10381 tctcatgcag gaataatacc gggtggagat gatgtgtcta ttttatggaa ccgtcgtaca
    10441 cctgagaaac tagacaaact acagatattc ggacatacgc cacttagaga atatgtttat
    10501 aagaaagaat attattctaa tattgatacc ggttgtgttt ttaataataa actgactgca
    10561 gttattgtag acataaatga agggattatt gtagatgttt tggatgaaaa ctgtgatgag
    10621 aacgatgtgg atccagaagc tgaaatggaa ctttaagaat aaaatttata aagcaaacta
    10681 atagtgaaaa gaaaaaatta aagaggcaaa aaccattaga ttaaaataga ttaaaataaa
    10741 tattttggaa aacatacatg taaaaaatgc cgagtatctt gaagaataca aaattaatgt
    10801 agaattcaat acaggattaa atcaggtcat agatctcaaa gacgaaattt gggaggaaat
    10861 atttgagcca cttaaagaca aggaatattt cagaaatttc aaactgaatc cctttacaat
    10921 atactgggaa aacggtgctg atttttcacc tgaatatctt tttgaaatgg caaaaaagca
    10981 ggaatttatg attgccgata aaaagcgaaa tcaaaattaa tcaaaccttt tatcaggcaa
    11041 aagatcattc ttgatttaaa gagattaatt cgtctgcttt actgcaaata cttataaata
    11101 tcctctaaga ttttcaataa aaatccgcaa tttcaattca tttcataaag aataatactt
    11161 atcaattcat tgtaaatttt acaagaaatg agtattttat gcaataaatt taattcccga
    11221 aactacagat tgaagatcaa tttttgttat cacaagccat tcaattcgat taaaataagt
    11281 aaatccaaaa atgaaaattc ataatccggt aagagcatat ttacgggata tggactcttt
    11341 tttttatgga ggtaacggat ggcaggccgg tctttttgag caggtaaagg atctgaattt
    11401 taagcaggca gtttggagac cagatattaa tagaaacagt atctggaaaa ttgtaaaaca
    11461 tacaattttc tggaaacaat atattttttc agaagaggaa ggcaaaccat tttcatcaga
    11521 agaaagaagg tcaggcaact ggatgtccat tcctgaaaaa cctgatgaag agaaatggca
    11581 gtcagaatta cttcttctcg aaatgaccca taaaaaaatg aagtcactta tcaggaaaaa
    11641 agatattgaa ttgtttaata cccgtaaaga attctcaaat tacatccggg aaataatttc
    11701 tcatgatgca tatcatgcag gtcagatcgg acttgtaaga gtattacagg gaattaaacc
    11761 agctaaatat taaagatata aaaactaaag ttccagttag attaattatt aatttattta
    11821 tcaaaaaatt atttatccgt gtaatatatt attacaaagg atttaaaatt ataatataat
    11881 atgccaaacg aaaaaatcat accaataaat atagaagacg aaatgaagtc ttcatacatt
    11941 gattattcaa tgtcagtaat agtatcaaga gctttacctg atgtgagaga cggtttgaaa
    12001 cctgtacaca gaagagtttt atttggaatg agagaactcg gtcttgcttc gaaccgtgct
    12061 cataaaaaat cagcaagaat agtcggagaa gtgttaggta aatatcatcc gcacggggat
    12121 aaggcagttt atgatacaat ggtaagattg gtacaggagt tttcactgag gtatccgctt
    12181 gttaacggac agggtaactt tggttcggtc gatggagatt cagcagcagc tatgaggtat
    12241 acagaagcaa gattatcaag agtcgcagaa gcgatacttg aggatcttga aaaagatact
    12301 gtagatttcg ttccgaactt tgatgacaca ttaacagagc cgtctgtcct gcctgcagtg
    12361 atcccgaatt tacttatcaa cggatccaat ggtattgcag tcggaatggc tacaaacatt
    12421 ccgccacata atctgaatga aatatgtaac gggataattc aattgataaa agatcctgag
    12481 attacaataa agaaattaat gaaatttgta actgctccgg attttcctac cggtggaatt
    12541 atttacgggt atcagcctgt gattgatgct tatgaaaccg gcagaggtaa aataatttta
    12601 agagcaaaag tttcaattga agtcgagaaa aacggtaagc ataatattat aatttctgaa
    12661 attccatatc aggtaaacaa agtaagtctg atagaaaata ttgcgcagct cgtaagagat
    12721 aaaaaagtag aagacatttc agatattaat gatgaaagtg acagagacgg aatgagagtt
    12781 gtgatcggtc tcaaacgtga cggtaatcct caggtgatcg tgaataatct ttacaagcat
    12841 acacagatgc agacaacgtt tggaactatc atgcttgcgc ttgttgacgg gattccaaaa
    12901 gttctgaatc tgaaagagat gatggagcat ttcatcaaac acagactgaa tgttatcgtc
    12961 agaagaacaa agtttgaact ggatgcagca gagaagagag ctcatattct cgaaggttac
    13021 attatagcac tggataatat cgatgagata atcaagctga tcaaaaaatc acctgatgct
    13081 cctactgcaa gaacagcatt gatgaaatca tttaagctct ctgaaataca ggcaacagca
    13141 attcttgaaa tgagactgca gagactgaca ggacttgaga gaaagaaaat tgaagatgaa
    13201 tataaagaag tactgatgac aattgagaga ttaaaaagaa ttcttgataa tgaacagctc
    13261 agaaaagata tgatctgtga tgatctgaaa gcacttgttg aaaaattcgg agatgaaaga
    13321 aggactgaaa tagtatatga tgtaagatcc atgactgatg atgagatgat gaaagagctg
    13381 gttaaagaag aagacgtagt aattacaatt tcgaataaag gatttattaa aaggattcct
    13441 gtcacatcat acaaatcgca gggcagggga ggcaaaggta ttactgctgc gtctatggga
    13501 agtgctgaag atgatttcaa tgagcacatg ttcattggat ctacacatca gtatataatg
    13561 ttttttacta aacttggaaa atgttactgg ctgaaggtct atgatattcc tgaaggaagc
    13621 agaacttcaa gaggtaaatc aatacttaat cttattgaaa aagataaaga tgatgacatc
    13681 tcttcttttg taatagttaa agattttacc gaagaacttt ttgttacgat ggtaacaaga
    13741 tcaggtgtta taaagaaaac gaaacttgac agctattcta atatcagaaa aagtggaatc
    13801 aacgctatca atcttaaaga tgatgataca cttgtagatg tgaaactgac aaacggtaca
    13861 caggaaattg tcattggaag tcataatggt caggcaatca gattcaatga aacaaaagcc
    13921 cgtgaaatgg gcaggactgc taccggagta aaaggtataa aacttgcaaa agacgattat
    13981 gtcataggct ttgtgactgt atcaagaccg agcgctacta tcctcgtgat cactgaaaac
    14041 ggatatggga agagaagcaa tgttggagat tacagagtaa ccaacagagg aggtaaagga
    14101 gtgatcacag tcaagactac tgataaagtc gggaaattga tctcaataaa agaagtaact
    14161 gacactgatg atcttatgat aattacttct aaaggaattt tgatcagaca gaatgtgaag
    14221 tcaatcaggg taatgggaag aaatgctcag ggagtaagag taatcagatt aaatgatggt
    14281 gatactatct ctgcagttgc tagaatagta gaagatgaaa aagttaagcc ggttgaataa
    14341 tatataaaat agaaattgga ttgagtaaaa taattgatat aagaagtgat acagtgacaa
    14401 agccttcacc ggcaatgctt gaagcaataa tgaatgcaaa acttggtgat gacgtatttg
    14461 gtgaagatga aagtgtgaat gagcttgaaa aaaaatgcgc tgatctttcc ggcaaggagt
    14521 cggcgatatt tgtgccgacc ggtgtcatgg gaaatcagct tgcaataaaa tgccacactg
    14581 atccgggcga tgaagttata gtcgaaagtg aatcgcatat tctgaattat gaaactgctg
    14641 caccttctgt aatttcgaat gttcagcttc ttcctgtacc cggtaagaat ggtgtaatta
    14701 cagcagaaga tattgagaaa tatatccgga caaatgaata ttattttcct gtgacaaaac
    14761 ttatttgtct ggagaatact cataaccgct cagggggagt catacagccg attgatgtta
    14821 taaaaggaat ttcggactta gcaaaaagat atggtatcag aacacatctt gacggagcaa
    14881 gaatatttaa tgctgtagtc gaaacaggaa tttcattaaa ggaatatgct gataattttg
    14941 attcaatttc attttgtttt tccaaaggac ttggctgtcc ggttggttca atactttgtg
    15001 gcagcaatga acttattgac aaagcccgca agtggagaaa aattctcggc ggcggaatga
    15061 gacaggcagg gattcttgca gcagcaggga tttatgcact tgataataat attgagcgat
    15121 taaaagaaga ccattctaaa gccaaatatt ttgcaaaaga gttatcaaaa ttagaaagcg
    15181 tatcaattga tatgtcaaca gtaatgacaa acattgtgat ttttggaagt gataaatatt
    15241 ccaaaaaaga aattatagat aaagcaaaaa ataacggtgt gataatttct tccggcagtt
    15301 atggattaat gagagcagtt tttcatttag atgtgagtat ggatgaaact aaaaaagcaa
    15361 ttgataaact gaaaaccatt ctatagaaat cccttccgag ttttttcaaa ttaagtaatt
    15421 ccttgcaatt gtaaatccat atatctaagt ttcaaaaaaa tttttaatat gataagcaga
    15481 agagatttta tcaggttttg ttcagtggct gcagtaggag cttctataat tcctttaata
    15541 gaaggctgca cagaaaagaa tattgagttt gggaatacag agtttatgaa actgagttct
    15601 gtacttacgg gattctcaga aactgaatta gataaaactc ttgcagcggc atatcagaca
    15661 agtcttacag actttcctcc tgctaaaaca actttaaaag aattatacat ttcacttaat
    15721 atagattcta ataagattcc ttcggatgaa gaaatcgaaa acgttattac aaaaaatcct
    15781 gagtataaaa aacttgcaga tactttgatc acatactgga tgtcaggaat ttacaaaaca
    15841 aaagacggaa tgaaagtatc tgattatgaa aaaatgtacg catggaaatc aacaggttat
    15901 cttattccaa atgcgcagtg cagaggagac tttggattct ggcagaataa acctgttgtt
    15961 acataattta actaaatatt atttatgcag aaaagtgatt acagtgcaga tgttgttata
    16021 gtcggtgcgg gaatttcagg agctttgatg gctcttggtc tggctaaagc aaatgcaaaa
    16081 gttcttatca ttgaagccgg cgataggatt gacagaagta aagctattga aaaatacaaa
    16141 gctgatccgg aaagaggtga tgactcacct tacaatcctg tatcacggac tgccccatat
    16201 ccaccgatgg atgatcctca gcaatattac attcagatgg gacctgataa atttcagagt
    16261 acatacataa aagctgtcgg cggaaccaca tggcactggc ttggcacttg tctgagattt
    16321 gtagaaaatg attttaaaat gaaaacggaa tacggagtcg ggactgactg gccaatcact
    16381 tataaagaaa ttgagaaatg gtattttgct gcagaacagg agatccctgt ctccggtgat
    16441 agcaatgcag cacttggtgt caaaagagaa aaaccgtatc cttccgctat gatcccgcag
    16501 acttatcttg ataagcaagt tgatattgct tttcagaatt taaatttgcc tgatgcaact
    16561 gatataaaac ttcaggtggc aagtactcct cagggcagac tctctgcgcc gcaggacgaa
    16621 agacttgcct gctgcggaag taataactgt attcccattt gtccggttgg cgcaaaatat
    16681 gatgcttcat ttacaattgc aaaagctgaa gcacttggtg taaaagttct cacaaactct
    16741 actgctaaat ttattcaaac tgatgatgag ggaagtgtaa caggaatttt atttcagact
    16801 gctgacggtg ataaaattgc tgctggtaaa atatttattc ttgcaggtca tgcgattgag
    16861 acacctaaat taatgctgat gtcaaaaact gaaaaatatc cgaacggtat tgcaaacagc
    16921 agtgatcagg tcggaagaaa tctgatggat cataactgcg ttctgagtta cggattaaca
    16981 aaagatcctg tttatccata tcaagctccg ctttctactt cgggagttga aaatgttaaa
    17041 gacggaccat tcagaaaaga aagaggtgct ttccggatag agattggaaa tgacggctgg
    17101 agctggcctg tcggatatcc aaatgtactg tcatcttatt ttttaaaaaa gagtggaggc
    17161 agatccagtg tcatgaacct tcctgttact gatgaagtga taaagcaagg acagaacaat
    17221 ttattatatg gcaaaaaatt acgaaatgat attaaggaaa atgtcttaag ggagatacga
    17281 tttgcatttc tttgtgagca gcttcccgat ccgaataaca gaatcgtgcc ggcttttgat
    17341 tctcttggca cagacggact tccgcgacca aggatctttt ataaattaga tgattatgta
    17401 aaagccagta ttgatgtagc tgtaactctg cagaaaagta tgcttgactc actcggctgt
    17461 acacagcagc atcactcata tccatgggga gcagggcata ttgtcggcac tacaaaaatg
    17521 ggggatgatc cgaaaacttc tgttgttgat aaaaatttga aatgccacga tcttaaaaat
    17581 atgtacatac ttgggagttc ggtctttcct actgttgctg cttctaatcc aactctgacc
    17641 attgctgcac ttactctgag agctgtagaa gaaatacaga aaaaactatt ggagcttaat
    17701 taaatataag atcagatcaa tccaagtgtt taagtgtatt aatgatcttt aatacatctt
    17761 cctttttatg cattggaaaa tttgcaatac gtatctgctc agtcttaaac tttccataac
    17821 ctgatccaac aattattcca ttatccgata aaagtttttt tacttctacc tgtctgcttc
    17881 ctgtttcaat tacaataata gtctttgatc tgttttcagt attttttaca aatagtgtaa
    17941 atgatctgtg tgtgttaaaa aaattatata gcatatcaga tttttcttct gtctcttttc
    18001 tgatctcgga aattcccttt tcattcaact gctcacagac ttttccaagc agatagatcc
    18061 caggcatatt tggtgtctca gtagtctgat acattaattc attttctaaa agcgaaataa
    18121 aattatgata gctgccaatt ttaattccat ctgatttcag cttctcagct ttttgcaaac
    18181 atctatcatt taaaatcaat actcccagtc ctgcgggaag tccaaatcct ttttgtacag
    18241 aaaagaaagc acagtcaatt ttactgaaat caatatttac ataaggcgca gaagttacta
    18301 tatcaacagc aataatagaa tccggatatt taattttaag atcataaata ttctgaacat
    18361 caattgccac tcctgtactg gtttcgtttt gagtaatgca gattacttca ggatcattat
    18421 caatttttat atcatcaaaa ttaaaactag atccataatc aacttcactc ttcacggcat
    18481 tgcgacccag ctcaactgca gttttataaa atctgtctgc aaaagctcca ttagcaaagt
    18541 ggaaagtatt tttcaccgta cagttttgaa tgattctgtc catacattcc gttgcagatg
    18601 ataaaaagaa tacatgatag ttttcaggaa tattcatcag agtcttcaga gatgaaacag
    18661 tatttttaaa tatttccatg aattcaatac ttcggtgatt taatgaaaat atttttttat
    18721 ccaaagcatc tgacagatcc tttttagcat tgggatataa ttccgtaggt ccgggagtaa
    18781 aataagtttt attcatctta ataaattatc aataaagtat tctgaattta atagtgtgag
    18841 gaattttttt cagatcctga atgacctgag gtgaatattt tttcttaatg tcagtgatca
    18901 catatccaac aagttcattg gttttcagat actgacctat gatgtttata ttatgtgaag
    18961 cgagaatgtt attgatctga gcaagtatcc ccggtacgtt ttcatgaata tgaattaacc
    19021 gatgtgcatt ttgaaactga ggtaattgta tctcaggaaa attaacactg taaaatgtgt
    19081 tacctgaatt tatgaattca ataattttct ttggaacgaa atcagcaatg tttctctgag
    19141 cttcttctgt actgccgccg atatgcggtg tcagtaatac gtttggaatg ttttgtaaaa
    19201 cagatacaaa tgcttcatca tttcccgacg gctcttccgg atatacatct acagaagcac
    19261 cgctgatctt accactgatc aaacattttg caagagcttc aatatcgact acatgtccac
    19321 ggcttagatt taaaaaaact gtaccatcac gcattccctt aaactctttt tcaccgatga
    19381 aatttgtatt ggaatctttt ccatccacat gtaatgtaat gatctcacat tttttcaata
    19441 attcattaag agaattacat ttagtagcat ttcccaaagc taatttatcg acaacatcat
    19501 aatagtaaac atccattcca agatcttcgg caagaactga taattgagat ccaattttac
    19561 cgtatccaat aatgccaatc ttctttccac gtatttcaaa acttccggct gcagatttat
    19621 cccagatacc tttgtgcaga gaagtgcttt tatcaaagat ccttctcatt aaaataatta
    19681 tctcagcaag tgcaagctca acgacactgc gtgtattgct aaacggtgca ttgaaaactg
    19741 ctatgccttt ttttaatgcg gtactgagat cgatctgatt agtcccgata caaaatgcac
    19801 ctatggaaat taatttatca gcatgatcaa ttacctgacg gcttatctga gtctttgaac
    19861 ggattccgag aattgaaaca tctttgatct ttttgcaaag ttctttctca tccatacttt
    19921 tagggaatga ttctatctga taaccttcct ctttgaaaat tgaaactgcg tctttgtgaa
    19981 tgttctcaag aagtaaaact tttatacgat tctttggata agaaatagtc atcggtaatt
    20041 tattcagata taaaaattca tccatgctcg gagtaatgtg atcagctttc gatgacacga
    20101 tctctctttc gacattttca gtgaaagcaa aaaacttgtg agctattcct gctttcttca
    20161 attcatagtc agtatatcca tctccaatga cgtaaagttc accggacagt ccaagctgtt
    20221 tgagctgttc gatctttcca ttatcaaagc taagaagatt ctttttatca tagcccgaaa
    20281 tatttccatc tttatcataa gtaaaagtat ttgcaaaaac attctcagca ggaattccaa
    20341 atttacctac taccggtact ataagttctt taaagccgac ggagataatg taaatgtttt
    20401 cggaatgatt tatgaagaac gatttatttc ttttaaccga agaagatact ttgcctgaaa
    20461 gctttttaac aagtttgtcg atatgagttc tgtcagcttt taaaagcttc atcctttttt
    20521 taagtgattc actgaatgaa agacttccgt tcattgagct tgaagtaatt ttctcaaatt
    20581 cctttagctc cagatctgat ttaatgcctt tgatattgga aatattgaag agttcgtcaa
    20641 gtgcttcaag ttttataaaa gtactgtcga aatctattat atacttaatt ttgcttttat
    20701 tcaaaaaata tattcagatt taagtttaaa actattatgt caaagattat tcaatttgaa
    20761 aattataaca actaaaaatt gaggcatgca gtccaaaaat aaaattatta agtttgtctt
    20821 aaagtatcaa tgaatcaatg attcaaagat tcaatgattt taaaaattta tttatttaaa
    20881 actttgatcc tttgagtttt agaggagaca atccgagcgg gagacgggga tcgaacccgc
    20941 gacgtccagc ttgggaagct gacattctac cgctgaatta ctcccgcaaa attctaaact
    21001 atcgctatta tcaagacaac aattaaaagt acaaggataa ttgtgatcaa agtataatgg
    21061 tcaaggtcca tagtattttc agcatagacg atctcacttt tattatttct ttctacaatt
    21121 ccaaaaatat ctttatccag atcaccatct ctgtcagcca tgaataccgg aaatggaatg
    21181 ctcacaggaa aaatagatct catttcataa ttatcaaaat ttttattctc aagtacaaat
    21241 tgaggtttat ctaaagagcc ggtatttttg taatattcta ctttcccatc attaaatttt
    21301 aatatatcga aatctccgtc ttcatcaatg tccaccatta cgggattatt gatattcaga
    21361 ttgttattta catattcctt tgcaagactt ttgatgcggc tttttgagta tttctgatcc
    21421 ggagaaatat caccagccag agaaataata gaagtaaaaa agatcaatac aaatgttagt
    21481 ataattgtta tcataatatt tacacgttta attagaaata atttttgtaa aaataactta
    21541 attcttttac agttaaaact taatagaaat tatactttgt tttgtcacat tggtatttaa
    21601 agattgtcta aacctgtata gaaagtaaat ttttttaata taatctctta gaataatgcc
    21661 caaaataatt ttgatattca ttacagcact ggtcttattt ggatgcagtc aaaagaatgt
    21721 acatgataaa caaacatcaa taaatgagat caaaaaagct gataaagaaa tgagcgctct
    21781 cgcagagcag gaaggttttt ccactgcact gctaaaaaac gctgatgaaa attttgtgaa
    21841 attatctgaa ggaattcatc cggtaatagg gaagagtgca tttgaagagt taacgaatgg
    21901 aaagcccggt cccaagactc taacgtggga agctgtattc actgatgttg cagagtcagg
    21961 agatctcggt tacagttggg gaaactggaa gtttgtactt cctgatacag ttttttatgg
    22021 gaattatttc actgtctgga aaaagtacaa tgacggcaca tggaaaatga ctttggatgg
    22081 tgggaatggt actccaaaac caaatgatta atgatcaacg ttcaattttt aatgttcact
    22141 gtgcaatatg caatggaggt gaatttgtct gaacctctga ttacactgat taaattgatt
    22201 tcgctgattc ggtaactctt tgattcctaa gtatagtgaa catgttaaga attaatgcca
    22261 gcgtcatcaa ctgttaagac aaaaaaaaca ccggctgtaa aaaacacaac cggtgaattg
    22321 aggagtgacg aatatacgga tcaaaatttt atttcagcaa aagcattctc ttgctatcag
    22381 ttgcaatttc attcacaaac at
//
LOCUS       DAORVQ010000029        21961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_890_length_21961_cov_5.673742, whole genome shotgun sequence.
ACCESSION   DAORVQ010000029 DAORVQ010000000
VERSION     DAORVQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 21961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21961
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_21961_cov_5.673742"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..5404
                     /locus_tag="PLX80_04160"
     CDS             125..5404
                     /locus_tag="PLX80_04160"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQY20024.1"
                     /translation="MKQGLLAIPDKNIFGSKTFEGLKDTLNVLLDTMKIDEIKLLNQF
                     KLDIDFACKSNLTEYQDIKYNLEILKKDFYLFIEQFKNNPNNKAHWWIQILKAQLDRL
                     NDDLQIFKPWDLLKNAPYVIINADPENSNKTLKELLESARKLQIQVNEQKRTNSLNGD
                     IEWYKLLTSSISQTIQLASEKIQTLENLAEQCNSLSDMEWDFLFDKSSNLFNIGYNAD
                     EHCSDKSYYDLLASEARLCPYLGIVQGKLPLQSWFALGRLLTRIDGNQILLSWSGSMF
                     EYLMPLLIMPTFENTLLDQTYKAVVEYQINYGRRLNLPWGISESGFNLLNANNEYQYR
                     AFGAPGLGLKRGLDNDMVIAPYASVLALMVDPANACENLEQLSKLGMNAQYGFYEAID
                     YTPSRLENGQDSAIIYSFMAHHQGMSLLSLANLLLDKPMQKYFKSEPQFKTGLLLLQE
                     RIPETTVFFAHTTEIPDLKYVGEEIQQRIFNTPDTPLPEVQLLSNGRYNVLVSNSGSG
                     YSFWKELAVTRWREDGTCDNWGSFCYIRDLKNNKFWSNSYQPTLRKGEKYEAVFSQDR
                     VDIRTVNNEIESHTEIAVSPEDDIEMRRLHIYNNSQFRKVIEVTSYAEVVLAPLSSDL
                     SGPAFSNLFVQTKILEDENAIICTRRPRSQDEQTPWMFHMMSLKDKVPEEISYESDRM
                     EFIGRGNSLVNPKVMQEVGPLSGNEGSVLDPIVAIRYKFILEKEETITIDMVTGIADT
                     KENCRGLIDKYQDRHNKDRVYELARTHSQVVLRQIDATEADAQLYEHLAGSIIFNNSN
                     FRADPSILIKNHRKQSDLWAYSISGDLPIILLRIVNKTNIQLIKKLLQAHTYWSLKGL
                     KVDLVIWNEEQSGYRQDFQKDIEALIPPETRNRPGGIFLRAADQISTEDRILFQTVAR
                     IYIVDNGRTLADITNLEQQKTSVIPRIKQEIRRYQIDTSISLPNDLIFFNGYGGFSSA
                     AAEYVIITDQKNRTPAPWVNVISNPKLGTVISESGSAYTWSENAHEFRLTPWSNDPVS
                     DSGGEAYYIRDDESGNFWSASLFPSGGKTPYITRHGFGYSKFEHIEDGIYSEMLVYTD
                     IEEAVKFTVLKIRNQSGRSRKLSATGYVEWVLGGNRTNTMMHIHTEIDPDSGALFAKN
                     PFSTEFRDRVAFFDVDEPNKSFTANRKEFIGRNGNLTNPDAMSRLNLSGTIGLALDPC
                     AAIQVPFVLADGDEHEIVFRLGAGQDFNNASLIAMKFRGKNVALNSFEKVKKYWSCTT
                     EVIKVETPDEAINLLTNGWLTYQTLSSRLWARSGFYQSGGAFGFRDQLQDVLSLLYTA
                     PQLAREQILLCASRQFKEGDVQHWWHPPVGRGVRTRCSDDFLWLPFVVSHYISVTGDT
                     QILDVPVRFLEGPLLNSDQESYYDLPAESGKSTKLYDHCIRAIRNGFKFGIHGLPLIG
                     TGDWNDGYDKVGNEGKGESVWLAFFQYDILIRFSEIANLQKDSEFAEECINKAQQLKS
                     NIDKNAWDGEWYKRAWFDNGTPLGSKVNDDCQIDSIVQSWSVLSGAGESDNINIAIES
                     AYNKLVEKETGIIKLLKPPFDMSKLNPGYIKGYVPGIRENGGQYTHAAVWLIKAFAEL
                     GDYKRVWELLNMINPINHGKTSETIAVYKVEPYVLAADVYSGMQHAGRGGWTWYTGSA
                     SWLYLAVLESFLGLKKESNKLKLNPCIPKDWKGFKISYRYNKTIYKINVTQISGTGNT
                     TVIIDGIDQKDNWITLVDDEFEHDVQVRYFVKA"
     gene            complement(5532..6263)
                     /locus_tag="PLX80_04165"
     CDS             complement(5532..6263)
                     /locus_tag="PLX80_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY20025.1"
                     /translation="MKEDLLKESFGTLSETINGLINLGYNHDFNVQKECLICQQKNIT
                     LSPEYFQIDKVYRFEGASNPEDQAILYAISSTKFGIKGTLVNGYGISSDETFSKLIEK
                     LQTNKTDVSKIFKTLDATPQRPEGNRVLNAPLVEISISEFIDQIKNETTWADSDRNSV
                     TIFKSETMRIVLIGLHENAELKPHKANGVISVQVIDGKINFITDQQSTIIEKGQMIAL
                     QENITHSVIALTESFFLLTLAMNRK"
     gene            6859..7119
                     /locus_tag="PLX80_04170"
     CDS             6859..7119
                     /locus_tag="PLX80_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HQY20026.1"
                     /translation="MKVTVWDTYVTKKDGTNMHFDIIAPEEICDQSVIHKYGKEYLIS
                     RGEDGQHLTSNECKRCHVEEIQPKWKSDIIEKGYYIVEMENC"
     gene            7204..7638
                     /locus_tag="PLX80_04175"
     CDS             7204..7638
                     /locus_tag="PLX80_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQY20027.1"
                     /translation="MFSKACEYGIKAAVYIAVISQKGKRVSLNEIAEEINSPVAFTAK
                     VLQQLTRKSIVDSVRGPAGGFEIKKSKANKIKLSKIVSAIDGDSIYKDCALGFKNCNE
                     RLPCSLHHRFVTIRNELKKMLEETSLLDLSKDINDGLTFLKR"
     gene            7691..8416
                     /gene="ric"
                     /locus_tag="PLX80_04180"
     CDS             7691..8416
                     /gene="ric"
                     /locus_tag="PLX80_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HQY20028.1"
                     /translation="MENLRTQSIGKFVANDYRTAAVFQQYGIDFCCRGGKSINEVCDN
                     KKISADELLAKLNEVSKQTSDQITDYKSWDLDLLADYIEKKHHRYVEKTIPVLKQFLE
                     KLCKVHGKDHPELFEINDHFIISAGELTTHMKKEELMLFPYIKKMSALKNEPGKTLKT
                     GEGSVQNPINMMMEEHSAEGDRFRKISELSDNFKAPADGCTTYNVTFEMLKEFETDLF
                     MHIHLENNILFPGAIKLEEELAS"
     gene            8538..9005
                     /locus_tag="PLX80_04185"
     CDS             8538..9005
                     /locus_tag="PLX80_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQY20029.1"
                     /translation="MDKPIKRVEELKPFSRDHHHTLLLCWKIKAGFKKNIPVNRIKAY
                     SDWFFKNHIIGHFEMEEEYLFPVLGRDHKLIQQAFEEHKKLTELFTDKTNIEESLNLI
                     PELLESHIRFEERTLFNEIQKAASADQLEKFQHIHSDEKFTDNLSDEFWLYEK"
     gene            9069..10823
                     /locus_tag="PLX80_04190"
     CDS             9069..10823
                     /locus_tag="PLX80_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQY20030.1"
                     /translation="MKNLMYKSFKVSLFITYFLIIFSLLNINQTTEACPYCTKTFYYE
                     LLSTRGNTLGGQELLASIMNQEGIAGVSSVTGENRQNMTQLTAGQNVVTPGNTKIPQE
                     FIEIMKRDEGLSIPQTSYVPQDATPDKRVSIELAEGETYIGRGVMFKGFSTNGSIPGP
                     TIIVEEGDIVEFTVVNKGTVSHGASIHAAYTQTSKYLGKIPAGETRSMVFRATVPGVY
                     MYHCAPGGHAIPMHIIFGQYGMMVVKPKKQYKMEEVMKKKPDVEIFLVQHELYSNGED
                     AVNSKPIYVLFNGKIFRYVDEPIKAKPGDFVRIYFLNAGPNLVSTLHIVGIIWDYVYW
                     QGNPNVVMPGGQSVIAGPADSWVIDFRMPPDEGAYTLLTHAVGSTDRGAIGLLICDKN
                     AETPLTVKSDGPVYSAEEMKDIKSKITRTISPFEPGSPDVDPPAIYGSETKEVTVKII
                     GNSFIPKVLHIAPGTSVKWINEDVFSFMEGEFSGIHTATSYEGPKTFFSPLLSHTESF
                     TQKFEDEGEYKYMCAPHPYMKGVIVVGETEDQISASSLGWIKWMSIISFLLVLCLFLV
                     LMSLRKRYSNAKNHAVAV"
     gene            10881..11288
                     /locus_tag="PLX80_04195"
     CDS             10881..11288
                     /locus_tag="PLX80_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20031.1"
                     /translation="MKTYNSILFKLTAFVKLIIGKLQIRSILIILLLFVHETLFAQNE
                     ILETGLFTYSDFIVIILILLIATVFIGMEKFDDPKYEIQNQIPYNNSQVITDSFISQD
                     NVFDKSVFSKLKIAVFITCILLIMYAALMLISF"
     gene            11364..11705
                     /locus_tag="PLX80_04200"
                     /pseudo
     CDS             11364..11705
                     /locus_tag="PLX80_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266076.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
     gene            11777..12310
                     /locus_tag="PLX80_04205"
     CDS             11777..12310
                     /locus_tag="PLX80_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY20032.1"
                     /translation="MKTILVFVSTVDGKITKSGDPHVKKWTSLKDQEYFKKIWSESQL
                     IILGSGTFDAEPITPPLNNLLVIMTRNISNYKKYEVLGHLEFSDKSPVELTEQFEKRG
                     FKTMIVAGGAHIATSFLKEQLIDELWLTIEPKIFGTGGNFVIEEDLDINLTLISFEKV
                     NEKGTLITKYSVDKTIK"
     gene            12326..12871
                     /locus_tag="PLX80_04210"
     CDS             12326..12871
                     /locus_tag="PLX80_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009750945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HQY20033.1"
                     /translation="MSEAGKNKFIHTIGHSTRTLEEFLEILQSFQIEMLADIRSFPGS
                     RKFPHFNKEALEIFLPSNNIQYFHIKKLGGRRKVKPDSKNISWRNSAFRGYADYMETQ
                     TFIEGISQLECIALIHKTAYMCSEAVWWRCHRAMVSDFLKVHGWEVKHIMGINKEVDH
                     PFTSPAKIVNGELSYYENSLF"
     gene            complement(13077..13487)
                     /locus_tag="PLX80_04215"
     CDS             complement(13077..13487)
                     /locus_tag="PLX80_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQY20034.1"
                     /translation="MGKLAVIKTKETDSSVEDFINSVKDEQKRKDSFEILNMMQKASK
                     EKPKMWGSSIIGFGKLIYKSPASGREVEWFKIGFSPRKANLTIYLIDLKMHEDSLKKL
                     GKHKQGGGCLYINKLEDIDQKVLEKMIAVSLIGK"
     gene            complement(13810..14361)
                     /locus_tag="PLX80_04220"
     CDS             complement(13810..14361)
                     /locus_tag="PLX80_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY20035.1"
                     /translation="MRKIIAAMNTTLDGNCDHTAGIPDEEIHQHYAELLNNAGAVLYG
                     RITYQLMEYWRDVLKKPTGEKAMDEFAVAIDRIPKIVFSRTLKNLDWETAKLADRDIK
                     EEVLELRQQPGKDIFVGSPGLISELTNLYLIDEYQLCVHPVIAGSGLPLFKNINEKII
                     LKLIKTKTFESRTIILYYEPIKK"
     gene            complement(14488..15288)
                     /locus_tag="PLX80_04225"
     CDS             complement(14488..15288)
                     /locus_tag="PLX80_04225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY20036.1"
                     /translation="MDIQNLNHKDIKFIHEMLPLGWEDVVPIFDWYTHTDFCYPVKVC
                     IDKKIVGIGAAIVHNKSAWLAHIIVHADYRNQGIGKELTQSLVDTSYSKGCETIYLLA
                     TELGEPVYKKIGFETETEYMIYTCDATNGKFKMDENIIAISNDFKKQILNLDRQVSGE
                     VRILVLEQHLSDGFLFLKDNKVQGFYIPNLGNGLIIATTNIAGIELMKLRLTLKDFAA
                     FPIDNLNGTAFMEQNNFKKDRSEKRMRLGKKRNWQPQNIYNSIGGNLG"
     gene            complement(15382..15834)
                     /locus_tag="PLX80_04230"
     CDS             complement(15382..15834)
                     /locus_tag="PLX80_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY20037.1"
                     /translation="MTKNKLPIMHNVGIVVESLDDAISFFTEIGLTLEGRAIIEGEWS
                     GRVTGLDSQQVEIAMMVTPDGHSRLELSRFLKPPTISDHRTAPVNSLGYLRIMFTVEN
                     IDELVSRLIKNGSTLVGEVVQYENTYRLCYIRGVEGLLIGLAEQLNVK"
     gene            complement(15862..16584)
                     /gene="bla"
                     /locus_tag="PLX80_04235"
     CDS             complement(15862..16584)
                     /gene="bla"
                     /locus_tag="PLX80_04235"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaB/IND/MUS family subclass B1
                     metallo-beta-lactamase"
                     /protein_id="HQY20038.1"
                     /translation="MRTFLIIFFTFTLTCVFGQTEEPKLKISHLKGDFYIYTTYSIYE
                     GSQIPANGMYLLTDDGVILFDTPWDTTQFQPLLDSIKLKHNKNVIICIATHWHSDRTE
                     GLEYYKQHGIKTYTTFLTDELSKENNKKRAEFLIANDTVFNAGQYSFETYYPGEGHTK
                     DNIVVWFGKEKILYGGCLIKGADAENLGYLGDANVREYETTLRNVQEKCPDPEFIIIS
                     HSDWNDINSLKHSIELAKKLKE"
     gene            complement(16619..17191)
                     /locus_tag="PLX80_04240"
     CDS             complement(16619..17191)
                     /locus_tag="PLX80_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY20039.1"
                     /translation="MRKIISFMHISLDGFVAGPNGEMNWIKINEGIFDHIGKRISKGD
                     TALYGRVTFQMMENYWPTAADKPNATRHDIEHSKWYEKVNKIVLSRSMKDAELTNTEI
                     INDNFSDRINEIKQSEGEDILLFGSPTATHALIQLNLIDGFWLFVNPVILGKGIPLFT
                     DIKDKINLKLLTTRQFTCGVTELSYIVVRQ"
     gene            complement(17237..17587)
                     /locus_tag="PLX80_04245"
     CDS             complement(17237..17587)
                     /locus_tag="PLX80_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQY20040.1"
                     /translation="MVTIDTFRKLALSFPETTEEAHFEKTSFRIKKKIFATYDDIKKR
                     ACIKLSETDQYVFSSADNTIIFPVDNKWGKQGWTIIEMSEVHKKLFAEALKSAYCEVA
                     PKKLMEIIRPNGKK"
     gene            complement(17596..17976)
                     /locus_tag="PLX80_04250"
     CDS             complement(17596..17976)
                     /locus_tag="PLX80_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY20041.1"
                     /translation="MTDEQLISEFQKIEDEFNTAVISNRVDEIKKCITSDWVLVDSQG
                     GIIPQEGFFSVLEQGLLSHSTMTKEILRVKVYGDIALVTGRGQNTGTWQGQPLEADEW
                     ITDVYKKENDKWLCVLTHLTPVKK"
     gene            complement(18182..19057)
                     /locus_tag="PLX80_04255"
     CDS             complement(18182..19057)
                     /locus_tag="PLX80_04255"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HQY20042.1"
                     /translation="MEIPKFDETFIPILEILKTGKVYKARELIEEVKTKFYSGLTDEQ
                     QKQETKSGDLLIDNRIAWGKSYLKKGGYVYFPERGHVQITEKGKNHSSDLSVKDLENE
                     STLFDFYKQEKRNSLSEDKIKISSPQDLIDEGFNKIESDVKIELLLKLKNIDPYFFEK
                     VVLRLLKKMGYGEFIETAKSGDGGIDGIINEDKLGLDKIYIQAKRFNEGKVREKDIRN
                     FIGAMSGDTNKGVFVTTSEFDEKALEKARSAHHKIICIDGQKLVSLMHEFDIGIQVKT
                     IYEIKQVDEDFFYEQ"
     gene            19654..20745
                     /locus_tag="PLX80_04260"
     CDS             19654..20745
                     /locus_tag="PLX80_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY20043.1"
                     /translation="MHQVPDLINDLIFIPYYIFLGAVLFIGLYLLIGKLIKLPFSLSV
                     WILNLFKKTRQMLNDLFSKPPVRKIDKSRRAFLTTTTALVSGYAFIGSTVGVLDSHDY
                     EIVYKDIKIDDLPEEIKGTTITLISDTHSGPYMDEEMMGVYAKAINDLRSDIILIPGD
                     LTNSSMLEVHPFVNAFKSLKADKGIYASLGNHDYFSNAEYVAKVVQNNSPIKLLRNDS
                     DIININGKDLCIMGLEDTRQSRSNQDNVIMKYLDTTIENAKSKLKEKNLDYYEIPKLA
                     LIHKPYFFEEMSDKNIDLILSGHTHGGQVVLAKLGPVNISFAGAVSKFISGLYESGSS
                     KMYVSRGIGSVALPIRYNCPPEITKITLV"
     gene            complement(20987..21232)
                     /locus_tag="PLX80_04265"
     CDS             complement(20987..21232)
                     /locus_tag="PLX80_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20044.1"
                     /translation="MNKIFNSKTLEIDSIVLTFHSKKLEIDSIVLTFHSKTREIDSIV
                     FTFHSKTRVESSKTRVINSKKGVLNSNRGSYIFENRS"
     gene            complement(21574..>21961)
                     /locus_tag="PLX80_04270"
     CDS             complement(21574..>21961)
                     /locus_tag="PLX80_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20045.1"
                     /translation="TDRNLNSGMYNYRLKQVDFNGSFEYYNLRNEVSIGIPVQFKLSQ
                     NYPNPFNPSTTIRYDLPFDGKVSIKIFDMSGKEAATLVNDVKSAGYYSINFNASSLAS
                     GIYYYRITAVSNGQNFIETKKMILLK"
BASE COUNT         7283 a         3572 c         3738 g         7368 t
ORIGIN      
        1 gttcaaaatg gagcggttca aagggcattt ttttaattgg tatgatacaa aaacactggc
       61 ccatttaagt tcaaaatata tttcaacagt tgatagtggc aaccttgccg gatatttgct
      121 tataatgaaa caaggcttac ttgctattcc cgataagaat atattcggat caaaaacatt
      181 tgaaggactt aaagatactt taaatgtact attggatact atgaaaatag atgagattaa
      241 actgcttaac caattcaaac tagatataga ttttgcttgt aaatccaatt taaccgaata
      301 ccaagatata aaatataatt tagaaatttt aaaaaaagat ttttatttat ttatagaaca
      361 atttaaaaat aatccaaata ataaagcaca ttggtggata caaattttga aagcacaact
      421 tgatagattg aatgatgacc ttcaaatttt taaaccctgg gatttattaa aaaatgctcc
      481 ttatgtaatt atcaatgctg atcctgagaa ttcaaataaa acgttgaaag aattattaga
      541 atcagcacga aaattacaaa tacaggtaaa tgaacaaaag agaactaata gtttaaacgg
      601 agatatagag tggtataaat tattaactag ttcaatatca caaactattc aactggcaag
      661 tgaaaaaata caaacattag aaaatcttgc tgaacagtgc aattcattat cagatatgga
      721 gtgggatttc ctttttgaca aatcaagtaa tctgtttaat ataggataca atgcagatga
      781 acattgttca gataaaagct actatgacct gttggcatct gaggcaaggt tatgtccgta
      841 tttaggaatt gtccagggaa aactcccact acagagttgg tttgctttag gacgtttgct
      901 gacccggata gacggtaatc agatccttct ttcctggagc ggctcaatgt ttgaatatct
      961 gatgccatta ttaataatgc ctacttttga aaatactctg cttgaccaga catataaggc
     1021 tgttgtagaa tatcagatta attatggaag aaggttaaat ttaccatggg gaatttctga
     1081 atctggattc aatcttttaa atgccaataa cgaatatcag taccgggcat ttggtgctcc
     1141 gggtttaggt ttgaaacgtg gtcttgacaa tgatatggta atagcaccat atgcatcagt
     1201 acttgcgtta atggtcgacc cggcaaacgc atgtgaaaat ctggagcagt tatcaaaact
     1261 tggaatgaat gcacaatatg gtttttatga agctatagat tacactccct ctcgtttaga
     1321 aaacggacaa gacagcgcaa ttatttattc atttatggca catcaccagg gcatgagttt
     1381 gttgtcactg gcaaatttgc tgcttgacaa acctatgcag aaatatttta aatctgaacc
     1441 acagtttaaa actggacttt tattgcttca ggaaagaatt ccggaaacaa cagtattctt
     1501 cgcgcataca actgaaatac cagacctgaa atatgtagga gaagaaattc aacaaagaat
     1561 ttttaatact ccggatacac cattacctga agtacaatta ctttcaaacg gcagatataa
     1621 tgtattagta tcaaactccg gatcgggata tagcttctgg aaagaacttg cggttactag
     1681 atggcgtgag gacgggacat gtgacaattg gggttcattt tgttatattc gggatttaaa
     1741 aaataacaag ttctggtcaa attcatatca acccacactc cgtaagggtg aaaaatatga
     1801 agcagttttt tctcaggatc gcgttgacat tcgaacagta aataacgaaa ttgaatctca
     1861 taccgaaatt gcagtctcac cggaagatga tatagaaatg cgaagattac acatttacaa
     1921 caattcacaa tttagaaaag tcatcgaagt tacaagctat gctgaagtag tacttgcgcc
     1981 tttatcatca gatttatcag gaccggcttt cagtaatctt tttgtgcaga caaagatcct
     2041 cgaggatgaa aatgcaatca tttgtacgag aagaccgcgt tcacaagatg aacaaactcc
     2101 atggatgttt catatgatgt cattaaagga taaggttcct gaggaaatat cttatgaaag
     2161 tgaccgtatg gaatttattg gacgcggtaa ttcacttgtt aatccaaaag tgatgcagga
     2221 agtgggtcca ctttcgggta atgaaggttc ggttttagat ccgatagtcg caatccggta
     2281 taaatttatt cttgaaaagg aagaaacaat tacaatagat atggttacag gtattgctga
     2341 taccaaggaa aattgtcgtg ggttaattga taaatatcag gacaggcata ataaagacag
     2401 ggtttatgaa ctggcaagaa cccatagcca ggttgttctc cggcaaatcg atgcaactga
     2461 agcagatgct caattatacg aacacttagc tggttccatt atatttaaca attccaattt
     2521 tcgagctgat ccttctattc tcataaaaaa tcaccggaag caatctgact tatgggcata
     2581 ttctatttca ggagacttac caataatact tttaagaatt gtaaataaga caaacattca
     2641 actgataaaa aaacttttac aggcacacac ttattggagt ttaaaaggat taaaagttga
     2701 tcttgtaata tggaatgaag aacagagtgg ttacagacaa gattttcaaa aggatattga
     2761 agctttgatt ccaccggaaa cgagaaatcg tcccggaggt attttcttaa gagctgcaga
     2821 ccagatatct actgaagatc gaatactatt tcaaaccgtt gcacgtattt atattgtaga
     2881 taatggaaga acattagctg atataaccaa tcttgaacaa caaaaaactt ccgttattcc
     2941 acgcataaag caggaaataa gacgatatca aattgatact tcgatttctc ttccaaatga
     3001 tctgatcttt tttaacggat atggtggttt ctcatctgct gctgctgaat atgttattat
     3061 cacagatcaa aaaaacagga ctcctgcacc atgggttaat gtaatttcca atcctaaact
     3121 tggaacggta atttcagaaa gtggatctgc ttatacatgg tctgaaaatg cacatgaatt
     3181 ccgacttact ccatggagta atgatccggt cagcgattcc ggtggtgaag catattatat
     3241 tcgggatgat gaaagcggga atttttggtc ggcttcatta ttcccatccg gaggtaaaac
     3301 accatacatt acccggcatg ggtttggata tagtaaattt gaacatattg aagatggaat
     3361 atactcagaa atgttagtgt atacagatat tgaagaagct gttaaattca cagtattaaa
     3421 aattagaaat caatccggca gatctcgtaa gctatcggca acaggttatg tagaatgggt
     3481 ccttggaggt aacagaacaa atacaatgat gcatattcat acggaaattg atcccgacag
     3541 tggagctctt tttgctaaaa atccttttag tacagaattc cgtgatcggg ttgcattttt
     3601 tgatgttgat gaaccgaata aatcgtttac cgctaatcgt aaggaattta ttggacgcaa
     3661 tggaaacttg accaatcccg atgcaatgtc aagattaaat ctctcaggta ctatcggact
     3721 ggctttggat ccatgtgcag ccattcaggt accgtttgtt ctggctgatg gtgatgaaca
     3781 tgaaatagtc tttagactag gtgcaggaca ggattttaat aatgcaagtt taattgctat
     3841 gaaattccgt ggaaaaaatg ttgcgctgaa ttcattcgaa aaagtgaaaa aatattggag
     3901 ctgtactacg gaagttataa aagtggaaac tcctgacgag gcaatcaatt tacttacaaa
     3961 tggctggctt acttaccaga cactttcatc cagactatgg gctcgcagtg ggttttatca
     4021 gtcgggaggt gcttttggat ttagagatca gctgcaggat gtattgtcac ttctatatac
     4081 agctcctcag ctggctcgag aacaaatttt attatgcgct tcacgtcaat ttaaagaagg
     4141 agatgtgcag cattggtggc atccacctgt cggacgcgga gttcgtacca gatgttctga
     4201 tgattttctt tggttgcctt ttgtggtttc acattatatt tctgttactg gagacacgca
     4261 aatattggat gtaccggttc gtttccttga aggacctctt ttaaattcag atcaggaatc
     4321 ctattacgat cttcctgcag aatcaggtaa atctactaaa ctttacgatc attgtataag
     4381 agcaatcagg aatggtttta aatttggcat acatggatta ccattgatag gtacaggaga
     4441 ctggaatgac ggctatgata aagttggtaa cgagggaaaa ggtgaaagtg tatggctggc
     4501 attttttcaa tatgatatac ttattcgttt tagtgaaatt gcaaatcttc aaaaggattc
     4561 ggaatttgcc gaggaatgta taaacaaggc tcaacaattg aaatcaaata ttgataaaaa
     4621 tgcctgggat ggagaatggt ataaacgagc ctggtttgat aatggaactc ctcttggttc
     4681 aaaagttaat gatgattgtc aaatagattc tattgtacag agttggtcgg ttttgtcagg
     4741 tgccggagaa tcagataata ttaatattgc aatcgaatca gcgtataata agcttgtaga
     4801 aaaagagaca ggaatcatta aattattaaa gccgcctttc gatatgtcaa aactaaatcc
     4861 ggggtatata aaaggttatg taccgggcat aagagaaaat ggcgggcaat atactcatgc
     4921 tgcagtttgg ttaataaaag catttgcgga attaggagat tataaacgtg tctgggaatt
     4981 attgaacatg ataaatccaa ttaaccacgg taaaacttca gaaacaattg cagtctataa
     5041 agtagagcct tatgttttgg cagcagatgt atattcaggt atgcagcatg ccggacgagg
     5101 cggatggaca tggtacactg gatctgcatc ctggctgtat cttgctgttt tggaatcgtt
     5161 tcttggtctc aaaaaagaat caaacaaact taaactaaat ccctgtattc ctaaagattg
     5221 gaaaggtttt aaaataagct acaggtacaa taaaacaatc tataaaatta atgttactca
     5281 aataagcggt acaggaaata caacagtaat aattgacggc atagaccaaa aagataactg
     5341 gattacactt gtagatgatg agtttgaaca tgatgttcag gttcggtatt ttgtaaaagc
     5401 ataagttagt tccattatta gatcaagaat gatccaaaga gtatgaactg aaatgacagc
     5461 cgacaggaga tgctgattaa atagttgcag tgggtattaa ctttaaacaa atagaaaatt
     5521 attattacag actattttct attcattgca agtgtaagca ggaagaaact ttctgtcagc
     5581 gctattacac tatgggtaat gttttcctgt aatgctatca tttgtccttt ttctattata
     5641 gtactttgtt gatctgtaat aaaatttatt tttccatcta tgacttgaac acttatcaca
     5701 ccatttgcct tatgtggttt taattctgca ttttcgtgaa gtccaataag cacaattctc
     5761 atagtttctg atttaaatat tgtgacagaa tttcggtcgc tgtctgccca ggttgtttca
     5821 ttctttattt gatcaataaa ttcagatatg ctaatttcta ctaagggggc atttaaaacc
     5881 cgattacctt ctggtcgctg cggtgtggca tctaaggttt taaatatctt agaaacatct
     5941 gttttatttg tctgtaattt ctctatcaat tttgaaaatg tttcgtccga tgaaatacca
     6001 tatccattta ccagggttcc cttaatgcca aattttgttg aagaaattgc atataggatt
     6061 gcctggtctt caggatttga tgctccttca aaacgataca ctttgtcaat ttgaaaatat
     6121 tcaggagaga gtgtaatatt tttttgttgg catattaaac attctttctg aacattaaag
     6181 tcgtggttat aacctaaatt gattaatccg tttattgttt ctgataatgt gccaaaactt
     6241 tcttttaaaa gatcttcttt catttttgaa aattaattat ttatataggt ttacatctaa
     6301 ttatctgaat gtcaatttta aggctaattg taaccgtgac tttattacaa cttcattaag
     6361 ataaatatat tttctttaat aacaaaattg tctttgttaa attaaacaat gtcttttagt
     6421 ttagtactgt cagcaatttg atgttctttc ttattctttg ttatttagag tagataaaat
     6481 agaataggaa tacaattttt taaatacttc acatgattta ttagatacca agatattcgg
     6541 ttatagaaaa taatgagtaa aattttaatg gctgaaatga aagttaaaga aaataaaatt
     6601 aattggacga acgccattca ccaataggct atgaagaatt agaagaaata ttacaaactt
     6661 ttcaaattta gagacagcag gattttattt cacatacaaa tatctgaaga aataaataat
     6721 acctaatgcg gacaaatttg tcctttatta tttcataaat tcttattaac tttgtacctc
     6781 ttgacacact gccaccaaca tcagtaatat tgaattttaa acaaactaaa aaacattttt
     6841 aaaccttaat attgaaaatt gaaagtaaca gtctgggata catatgtaac taagaaggac
     6901 ggcactaaca tgcattttga cataattgct ccagaagaaa tatgcgatca atcagttatt
     6961 cacaaatacg gaaaagagta tttaatatcg agaggagagg atggtcagca tttgacttca
     7021 aatgaatgta aacgttgcca tgtagaagag atccagccga agtggaaatc tgacattatt
     7081 gaaaaaggat attacattgt ggaaatggaa aactgttgat aaaaaaaatc gggaaagtaa
     7141 gacaaaaata tactgtctta aatttaattt aaaattaaaa ttaaaattca aattaaaaaa
     7201 taaatgtttt caaaagcctg tgaatacgga ataaaagcag cggtatatat tgctgtcata
     7261 tctcagaagg gaaaaagagt aagcttaaat gagattgcag aggagataaa ttctcctgta
     7321 gcattcacag ctaaggtcct acagcagttg acaagaaaaa gtattgttga ttctgttcgt
     7381 ggacctgccg gaggtttcga aattaaaaaa agcaaagcaa ataagatcaa gctgagtaaa
     7441 atagtctcag cgatagacgg agattccata tacaaagact gtgcacttgg atttaaaaat
     7501 tgtaatgaaa gacttccatg ttcactgcac catagatttg taacaataag aaatgaattg
     7561 aaaaagatgc ttgaagaaac atctttattg gatttatcta aagacattaa tgatggactg
     7621 acatttttaa aaagataaac aagaaatttt aattaaatat tttatcaact attaaatcaa
     7681 aaaataaaaa atggaaaact taagaacaca aagcattgga aaatttgtag caaatgatta
     7741 ccgcacagcc gcggtattcc agcaatacgg aattgacttt tgctgcagag ggggtaaatc
     7801 tataaatgaa gtttgcgata ataagaaaat atcagccgat gaattactcg ctaagctgaa
     7861 tgaagtttca aaacaaacct cagatcaaat tacagattat aaatcatggg atctggattt
     7921 acttgctgat tatatagaga aaaaacatca cagatatgta gaaaagacaa ttcctgtatt
     7981 aaagcagttt cttgaaaaat tatgcaaagt tcatggaaaa gatcatcctg aattatttga
     8041 gattaatgat cactttataa tctctgcagg agaattaaca acgcacatga aaaaagaaga
     8101 actcatgctt ttcccttata taaaaaagat gtcagcttta aaaaatgaac cgggaaagac
     8161 attaaaaacg ggagaaggat cagttcaaaa tcctatcaat atgatgatgg aagagcacag
     8221 tgcagaagga gacagattca gaaaaatatc agagctgagt gataatttta aagctcctgc
     8281 tgatggctgc acaacttata atgtaacttt cgagatgtta aaggaatttg agactgatct
     8341 gttcatgcat attcaccttg agaataacat actgtttccg ggagcaataa aattagaaga
     8401 agagttagct tcataaattt aaataaataa cataccgccg gagtctcctg aaatgtagtg
     8461 ctcgctcagg cggtattagt ttttattttt ttacaatccg gtcaaaaatt tacaaatgaa
     8521 acctatataa ttttatcatg gacaaaccaa ttaaaagagt agaagagctt aaacctttta
     8581 gcagagatca tcaccataca ttacttcttt gctggaagat caaagctgga tttaaaaaaa
     8641 atattccggt aaacagaata aaagcatatt cagactggtt ttttaaaaat cacattatag
     8701 ggcattttga aatggaagag gaatatttat ttccggtttt aggaagagat cataaattaa
     8761 ttcaacaggc atttgaagaa cataaaaagc tgacagaact ttttactgat aaaaccaata
     8821 ttgaggagtc tttaaattta attcctgaat tgctggaaag tcatatccgg tttgaagaaa
     8881 ggactttatt taatgaaatt cagaaagcag catctgcaga tcagttggaa aaatttcaac
     8941 atatccattc tgatgagaaa tttactgata atcttagtga tgaattttgg ctatatgaaa
     9001 aataatattt agtactaaga ttactaattt ttacaatttt gtattaaaca ataaattaaa
     9061 ataagatcat gaaaaacctt atgtataaat cttttaaagt aagtttattt atcacttact
     9121 ttcttataat attttcttta ttaaacataa atcaaacaac cgaagcttgt ccatactgca
     9181 ccaaaacatt ttattatgag ctgcttagta ccagaggcaa cactcttgga ggacaagaat
     9241 tacttgcttc cattatgaat caggaaggaa ttgccggagt atcttccgtc actggagaga
     9301 acagacagaa tatgactcag ttaacagcag gacaaaatgt tgttactccc ggaaatacaa
     9361 aaatcccaca ggaatttatt gagataatga aaagagatga aggactttca attccgcaga
     9421 caagttatgt acctcaggat gccacaccgg ataagagagt ttcaatagaa cttgcagaag
     9481 gtgaaacata tattggcaga ggtgtcatgt tcaaaggatt cagtacaaac ggttcaatac
     9541 ccggtcccac tataattgta gaagaaggtg atattgttga gtttactgtt gtgaataaag
     9601 gaacagtttc tcacggtgca tctattcatg cggcatatac tcagacctca aaatatcttg
     9661 ggaagattcc tgcaggtgaa acaaggtcaa tggtattcag ggctactgtt cctggagtat
     9721 atatgtatca ctgtgcaccc ggaggtcatg caatcccaat gcatataata ttcggtcaat
     9781 acggaatgat ggtagtaaaa ccgaaaaagc aatataagat ggaggaggta atgaagaaga
     9841 aacctgatgt ggaaatattc cttgtgcagc atgaattata ctcaaacggt gaggacgcgg
     9901 ttaacagcaa accaatatat gttttgttca acggaaaaat attcagatat gtagatgaac
     9961 cgataaaagc aaagccgggt gattttgtta ggatttattt tttaaatgca ggaccaaatc
    10021 ttgtatcgac tctgcacata gtaggtatca tctgggatta tgtttactgg cagggaaatc
    10081 caaatgtagt catgcccggg ggacagtctg taattgcagg tcctgctgac tcatgggtga
    10141 ttgatttccg catgccgccg gatgaaggcg cttataccct gctcactcat gctgttggtt
    10201 cgaccgacag gggggcaata ggtttattaa tttgtgataa aaatgctgag actccattga
    10261 cagtaaaatc cgacggtccg gtttactcag ctgaagaaat gaaagatata aaatctaaga
    10321 ttacaagaac tatatctccg ttcgagcccg gaagtccgga tgtagatccc ccggcaattt
    10381 atggttctga aacaaaagaa gtaacagtaa aaataatcgg caattctttc attccaaaag
    10441 ttttgcatat agcacccgga acttcagtaa aatggatcaa cgaagatgtc ttctctttta
    10501 tggaaggtga attttcagga atccatactg ccacttcata cgaaggaccg aaaacatttt
    10561 tttcaccatt gttatctcat acagaaagtt ttacgcaaaa atttgaagat gaaggagagt
    10621 ataaatatat gtgcgctcct catccttata tgaaaggtgt gatagtcgtt ggtgaaactg
    10681 aagaccagat atctgcttca tctttaggat ggatcaaatg gatgagcata atttcttttt
    10741 tactggtgct ctgcttattt ttagttttga tgtccttgag aaaaagatat agtaatgcaa
    10801 aaaaccatgc agttgctgta taaattttat aacactatat atttggatta tccaaaatat
    10861 atattattaa cattattatt ttgaaaactt ataactctat cttatttaaa ctgacagctt
    10921 ttgtaaaact aattattggg aagctgcaga tcagatcgat tctgataata cttttacttt
    10981 ttgttcatga aacattattt gcgcaaaatg aaattcttga gaccggctta tttacatatt
    11041 ctgattttat agttataata ttgatactcc tgattgcaac tgtttttatc ggcatggaga
    11101 aatttgatga tccgaaatat gaaattcaga atcagatacc ttataataat tcacaggtaa
    11161 taacggactc ttttattagt caggataatg tttttgataa aagtgttttt tccaaattga
    11221 aaattgctgt ttttattaca tgcattttgc tcattatgta tgcagctttg atgctgatta
    11281 gcttctgatt aaaattattt gaataacttg atttactttc aaatttataa ttacagatca
    11341 atttacttac agtatacatt taaatgaaaa ttaagattaa aagagtatat gaatctgccg
    11401 taaaagaaga tggttttaga attcttgttg acagacttta gccgagggga ttatcaaaag
    11461 aaaaagcaaa agttgatatg tggttaaaag aaattgctcc ctcaaccgaa cttagaaaat
    11521 ggtttgctca cgatcctaat aaatgggaag aattcaagaa acattatcta cttgaactca
    11581 aagaaaatca ggaagctgtt ttagaattaa aagaacgaat gaagatggaa gatctgacat
    11641 tagtttttgg cgcaaaagat gaagtacata gtgaagcagt tgtgctcatg aaaaaattaa
    11701 aataataata ataaaacaga tatctttata tacttagcag attgagtgag aatgaggtaa
    11761 taattaaata aaaaaaatga aaactatact tgtttttgtt tctacagtag atggaaaaat
    11821 cactaagtca ggtgatcctc atgtgaagaa atggacttct ctaaaagatc aggaatattt
    11881 taaaaaaatc tggagtgaat cacaactgat tattctcggc agcggaactt ttgatgcgga
    11941 acctattact ccaccattaa ataatctact tgtaataatg acaagaaata tttcaaatta
    12001 taaaaaatat gaagtcttag gtcatctgga atttagtgat aaatctcctg tcgaattaac
    12061 tgagcaattt gaaaaaagag gttttaaaac tatgatagta gccggaggtg cacatattgc
    12121 cacatccttt ttaaaagaac aactgataga tgagctatgg cttaccatag aacccaaaat
    12181 cttcggtacc ggaggcaatt ttgtcattga agaggatctc gacatcaatc ttacattaat
    12241 cagttttgaa aaggttaatg agaaaggtac actgataaca aaatattcag ttgataaaac
    12301 catcaaataa aatatttatc attcaatgag tgaagcaggc aaaaataaat tcatccacac
    12361 tattggtcat tcaacaagaa ctcttgaaga atttctggaa atattacagt cctttcagat
    12421 tgaaatgctg gcagatatca gaagttttcc gggttcacga aaatttcccc attttaacaa
    12481 agaagcactt gaaattttcc tgccgtcaaa taatattcaa tactttcata taaaaaaatt
    12541 aggcggaaga aggaaagtaa aaccggattc aaaaaatatc tcatggagaa attctgcttt
    12601 tcgtggttac gcagattata tggaaaccca aactttcata gaaggaatct ctcagcttga
    12661 atgtattgct ttaattcata aaactgcata tatgtgttca gaagcagtgt ggtggcggtg
    12721 tcaccgggct atggtctctg attttttaaa ggttcatggc tgggaagtaa agcatattat
    12781 gggaattaat aaagaagtag atcatccttt tacatcacct gcaaagattg taaacggaga
    12841 attgtcttat tatgaaaata gtttatttta atttaaatgg tagaaaataa aaacattttg
    12901 aaacgcacaa aatgacctta ctcaatttga ttaaacggta taaccgatgt aatgagctct
    12961 ttagacccag atttttcatt aaaaaatttt acacccgttc tcaaactccc tgatagaact
    13021 tgtatgcgaa atcccgtttt gtaaaatcca attacaggta tgttttactt aaaagatcat
    13081 tttcctatta atgatacagc tatcattttt tcaagtactt tttgatcaat atcctccagc
    13141 ttatttatat acaggcagcc cccaccctgt ttatgcttac ccagtttttt taaagagtct
    13201 tcatgcattt ttagatctat cagatatatt gtgaggtttg cttttcgggg tgaaaatcca
    13261 attttaaacc attcgacttc acgtcctgaa gcgggacttt tataaattaa ttttccaaaa
    13321 ccaataattg aactgcccca catttttggt ttttcctttg aagctttttg catcatattc
    13381 aaaatttcaa aactgtcttt acgtttttgt tcatccttta cgctgttaat aaagtcctct
    13441 acacttgagt cagtttcttt tgtttttatt actgctaatt ttcccataat gaatttttaa
    13501 attttaatat ttaaataaag taataataga taaataaaat aaatcttttt aaaattgtat
    13561 ttgaaaaatg aattaaagtt tatgttaatc agtatatctt gcaggctaaa acgattttag
    13621 tattctgatt tttaattttt tgtaaagtag atgttttaaa aatgtaatct gaattacaat
    13681 ttgcaaaaca aattttgaac aacttcaaac attatagtct ccacaaacac ttgccaattt
    13741 taaataaaac aaataaatta tttttgtgtt aaaacaactc ttcgtcaaaa atatttctca
    13801 tataaacagt cactttttaa ttggctcata ataaagaatt attgtccttg attcaaaagt
    13861 ttttgtctta atcagcttta agataatctt ttcatttatg tttttaaata agggcagacc
    13921 acttcctgct attacaggat gtacacaaag ttggtattcg tcaattaaat atagattcgt
    13981 taattctgaa attaaaccag gactgccaac gaaaatatct ttacccggtt gttgcctgag
    14041 ttctaaaact tcttctttaa tatctctgtc tgccagctta gcagtttccc aatctaaatt
    14101 tttcagcgta cgggaaaaaa caattttggg aatcctgtcg attgccacgg caaattcgtc
    14161 catagctttt tcaccggtag gttttttcaa aacatcccgc cagtattcca ttagctgata
    14221 tgtgatcctt ccatacagaa ctgcgcctgc gttatttaaa agttcagcgt aatgctgatg
    14281 tatttcttca tcaggaattc ctgctgtatg gtcgcaattt ccgtcaagtg tcgtattcat
    14341 tgcagcaatt atttttctca tacttattta ttaattacaa aagttaaatt tcataggatt
    14401 gcctcaaagc ctataagact caaaggctca aagtttaaaa ctttaaaatt tattttctca
    14461 gagacttaga ggcaaactta atatttctca acctaaattt ccaccaatac tattgtaaat
    14521 attttgaggc tgccagtttc ttttctttcc taatctcatt cgcttttctg aacggtcttt
    14581 tttgaaatta ttttgttcca taaatgctgt tccatttaaa ttgtcaattg gaaacgcagc
    14641 aaaatctttt aatgtcaaac gtaacttcat tagttctatt cctgctatat tagtagtcgc
    14701 tattattaaa ccgttaccta aattcggaat ataaaatccc tgtactttgt tgtcttttaa
    14761 aaataaaaat ccatctgaaa gatgctgttc taaaactaaa attctgactt ctcccgatac
    14821 ttgtctgtct aaatttaata tctgtttttt gaaatcattg ctgatcgcaa taatattctc
    14881 atccatcttg aattttccat ttgttgcatc acatgtataa atcatatatt cagtttctgt
    14941 ttcaaaccct atttttttat acaccggctc gccaagttca gtagctaata aataaatcgt
    15001 ctcacagcct tttgaatatg aagtgtcaac caaagactga gttaactctt ttcctatacc
    15061 ttgatttcta taatcagcat gaacaataat gtgagcaagc catgcagatt tattatgaac
    15121 tattgccgcc ccgatcccga caattttttt gtctatgcat acttttaccg gatagcaaaa
    15181 atccgtgtgt gtgtaccagt caaagatcgg aacaacgtct tcccagccta atggaagcat
    15241 ttcgtgaata aattttatgt ctttatgatt taaattttga atgtccaatt atataattta
    15301 tatagttctt tcatttttaa tatcaagagt tagttgtttt attaaataaa cttgtatata
    15361 aaatttaata tttaatattt attatttaac atttaactgt tctgccagtc cgataagaag
    15421 tccttctact ccccgaatat aacagagacg atacgtgttt tcgtattgta ccacttcgcc
    15481 taccagtgtt gaaccatttt taattagtct ggaaacaagc tcgtcaatat tttcaactgt
    15541 gaacataatg cgaagataac cgagggaatt aacaggtgct gtccgatgat ctgaaattgt
    15601 tggcggctta agaaatcgtg aaagctcaag tcggctgtga ccatcaggtg ttaccatcat
    15661 agcaatttcc acctgttgag aatccagtcc ggtaacacga ccagaccatt caccttcaat
    15721 tatcgcacgc ccttcgagtg tcagaccaat ttccgtgaaa aatgaaatgg cgtcatcaag
    15781 tgattctacc acgataccga cattgtgcat tataggtaat ttattttttg tcataatttt
    15841 ttctcttata aaattaaagt tttattcttt tagttttttt gccaattcta ttgaatgttt
    15901 taatgagttt atatcattcc agtcgctgtg tgatatgata ataaattccg ggtcagggca
    15961 tttttcctgc acattcctca gagtagtttc atattctcgg acattcgcat ctcctaagta
    16021 tcctaagttt tcagcgtcag cacctttaat cagacaacct ccatatagta ttttctcttt
    16081 gccaaaccaa actacaatgt tgtctttagt atgtccttct cccgggtaat aagtttcaaa
    16141 tgaatattgt ccggcattaa aaaccgtgtc gtttgcaatt aaaaattcag cccttttttt
    16201 attgttctct ttactcaatt catctgtcag aaaagttgtg tatgttttaa ttccgtgctg
    16261 cttataatat tcaagtcctt cagttctgtc actgtgccag tgcgttgcaa tacaaattat
    16321 cacattttta ttatgcttta attttatgct gtcaagcaat ggttgaaatt gtgtagtgtc
    16381 ccagggagtg tcaaataaaa tcacaccatc atctgtaagc agatacattc cgtttgcagg
    16441 tatttgactt ccttcataaa tgctgtatgt tgtataaata taaaagtcac cctttaagtg
    16501 tgagatcttt agtttgggtt cttctgtctg tccaaaaaca caggtcagag taaaagtaaa
    16561 aaatattatt aaaaatgtcc gcattattat atttaaaaag tttgatattg gttcgatgtt
    16621 attgtctgac aacaatataa ctcagttcag ttaccccgca ggtaaattgt cttgtagtca
    16681 aaagttttag attgattttg tctttgatat ctgtaaacag tggaattcct tttccaagaa
    16741 taaccggatt aacaaacagc cagaagccgt caattaagtt cagttgtata agggcatgtg
    16801 tagcagtcgg acttccaaaa agcaggatgt cttcaccttc actttgtttt atttcattta
    16861 ttctgtccga aaagttgtcg ttgataattt ctgtgtttgt caattccgca tctttcattg
    16921 atcttgataa tactatcttg tttacttttt cataccactt tgaatgttca atgtcatgcc
    16981 tggttgcatt tggtttgtct gctgcggttg gccagtaatt ttccatcatc tgaaaagtta
    17041 cccgtccata taatgcagtg tcacctttgc ttatccgctt tcctatatga tcaaaaattc
    17101 cctcattaat tttgatccag ttcatttctc cgttcggccc tgctacaaat ccatcaagcg
    17161 atatgtgcat gaatgaaatt atttttctca tatagtttta tttgttattg ttgtctgaga
    17221 tttattacgg tagtatttat ttttttccat taggtcttat tatttccata agctttttgg
    17281 gtgcaacttc acaatatgca cttttcaatg cttcagcaaa aagtttttta tgaacttcac
    17341 tcatttcaat aattgtccag ccttgtttgc cccatttatt atcaaccgga aaaattattg
    17401 tattatctgc agatgaaaaa acatattggt cagtttccga taatttgatg caagctcttt
    17461 tctttatgtc atcataagtt gcaaagattt tcttttttat tctgaaagat gttttttcaa
    17521 aatgagcttc ttctgtcgtt tccggaaacg aaagtgctaa ttttctgaat gtgtcaatag
    17581 ttaccatttg tcaaattatt tttttaccgg tgttaaatgt gtcaatacac aaagccactt
    17641 gtcattttct tttttatata catctgtaat ccattcgtcc gcttccaatg gttgtccttg
    17701 ccaggttcct gtattttgtc cgcgacctgt caccaaagca atatcaccat atactttaac
    17761 ccgtaaaatt tcctttgtca tcgtagaatg tgaaagcaaa ccttgttcga gaacgctaaa
    17821 aaatccttct tgcggaatta ttccgccttg actgtcgacc aaaacccaat cgcttgtaat
    17881 acattttttg atctcgtcaa ctctgttaga aattacagct gtattgaatt cgtcttctat
    17941 tttttgaaat tctgaaatta attgttcgtc tgtcaaaatt atatttagtt gtttgcaggt
    18001 tgtcttttgt tataagttcg ttgtgtttac ttttaaaatg actgaaaacg ttttggagtt
    18061 accctaagga tgcattctta cttataaact aaatttgaaa aactaaactt caaataatga
    18121 caaacttttc acatgaacac taaactccac tttttgggta gttcctgtta tgagctgtaa
    18181 tttattgttc ataaaaaaag tcctcgtcaa cctgctttat ttcatatatt gtttttactt
    18241 gaatacctat gtcaaactca tgcataagcg aaactaattt ctgaccgtca atacatatga
    18301 ttttatgatg agctgaacga gctttttcta atgctttttc atcaaactct gaagttgtga
    18361 caaatactcc tttatttgta tcaccgctca tggcaccaat aaaatttcta atgtcttttt
    18421 cccttacttt gccttcgttg aatcgttttg cctggatgta aattttgtcg agcccaagtt
    18481 tatcctcgtt aataatacca tcaattccac catcaccgga ttttgcagtt tcaatgaatt
    18541 ccccatatcc catttttttt aatagtctta agactacttt ttcaaaaaag taaggatcaa
    18601 tatttttaag ttttagaaga agttcgattt ttacatcgct ttcaattttg ttaaaacctt
    18661 catctattaa atcttgggga gatgaaattt taattttatc ttcactcaaa ctatttcgct
    18721 tttcttgttt gtaaaaatca aacaaagtac tctcattttc taaatctttt acagataaat
    18781 ctgaagaatg gtttttacct ttttctgtaa tttgaacatg tccacgttca ggaaaataaa
    18841 catagccacc cttttttaaa tatgattttc cccatgcaat tctattgtcg attagcagat
    18901 ctccggattt agtttcttgt ttctgctgct cgtccgtcag acctgaataa aattttgttt
    18961 taacttcctc tattagttct ctagccttat atactttacc agttttaaga atttcaagaa
    19021 tcggtataaa tgtttcgtca aattttggaa tttccatttt tatttcgatg tcgttttata
    19081 aaaatatcta aaaacaatta aatatatgaa ataaacaaat tatcttatgc ttaaatacaa
    19141 ctctcaaact gttgatttcc gcataacttg attgattgta ttcataatat cttatcaaaa
    19201 gccgattgaa ttttacttaa atgtttcttg cttttgtgag tataaatcat cctttcatat
    19261 ccaaaaattc agccaaaaat atattaaaat caaaaagaag ttacaatact gaaaaccaat
    19321 gccgtattct actctttatc agatacggac ttttttctat tttgtatgca gtatagaatg
    19381 tctttatttt aaaattattc ataatttgaa acgtattaca tttacgggag gttaaacttt
    19441 aaaaaatttt taaacaattt ttaaacaatt catccattgt ttcttacaat attcttatcg
    19501 gtttttttcg gacttcagat tcttgttttc attaccttca gacgatttgt taaatcaaaa
    19561 tatccggatt ccggattttt aaaaataatc tcaaattact cctttattgt tttaaacctt
    19621 ccgatgattt acattttatt caacaggaga caaatgcatc aggtacccga tcttatcaat
    19681 gaccttatat ttattcctta ttatatattt cttggcgctg ttttatttat aggtctgtat
    19741 ttgcttatcg gaaagttgat caaactgcca ttctctctat ccgtatggat cctgaatctc
    19801 ttcaaaaaga caagacaaat gttgaatgat ttattcagta aaccgcctgt ccgaaaaatt
    19861 gacaaatcaa gaagagcttt tttaactact actactgctt tggtatcagg ctatgcattc
    19921 atcggatcaa ctgttggtgt gctcgacagc catgattacg aaatagttta taaagacatt
    19981 aagatagatg accttcccga agaaatcaaa ggtacgacca ttacgctgat cagcgatacg
    20041 cattcaggtc catacatgga tgaagaaatg atgggagtat atgcgaaagc aataaatgat
    20101 ttgagatctg atataatatt gatacccggt gatctgacca attccagtat gctggaagtt
    20161 catccttttg taaatgcatt taaaagtttg aaagcggata aaggaattta tgcttcactc
    20221 ggaaatcatg actatttttc caatgcagaa tatgttgcaa aggtggttca gaacaatagt
    20281 ccaataaaac ttttgagaaa tgattctgat attataaata ttaatggtaa agatctgtgt
    20341 atcatgggtc ttgaggatac gaggcagagc cggtcaaatc aggataatgt aataatgaaa
    20401 tatcttgata ccacaattga aaatgcgaag tctaaattga aagagaaaaa tcttgattac
    20461 tatgaaattc caaaacttgc attaattcac aagccatatt ttttcgagga aatgtcagat
    20521 aagaatattg atctgatcct gagcggacat acacatggtg gtcaggtggt cctggcaaag
    20581 ttaggtcctg taaatatttc ttttgcaggt gctgtcagta aatttatcag cggcttgtat
    20641 gaatccggaa gttctaagat gtatgtatcc agaggaattg gaagtgtagc attacctatc
    20701 agatataatt gtcctcccga gattacaaag ataactttgg tctgatcaat aatactggtg
    20761 ctataaaatt tatagtggaa aataataaag taaatatttt tttattttcc acaaattttg
    20821 tcattcccga atgctactgg cgggaatcta atcatcacga aaactcaatt gatatatctg
    20881 atcttttcat tttatattta tgaaaattac taataattca aaaaattaaa gtgcttcaat
    20941 atcttaaaac ttaagaattg atttgttttt ccatttccat aaattctcaa cttctgtttt
    21001 cgaatatgta acttcctctg ttcgaattta aaacgccttt cttcgagttt ataacgcgtg
    21061 tcttcgaact ttcaacgcgt gtctttgaat gaaatgtgaa tactattgaa tcaatttcgc
    21121 gtgtctttga atgaaatgtg agtactattg aatcaatttc gagtttcttt gaatgaaatg
    21181 tgagtactat tgaatcaatt tcgagtgtct ttgaattaaa aattttattc actgcgataa
    21241 ttttcctacc actgcaacta aaaaattttg ctcttgtaac tactttttta gactttgttt
    21301 caaaaaattg tttctttgtt ttgatttcag atggttttga tattgggtaa gattttcaaa
    21361 cgattttgga gcacagatta gaaatctttc tcaccggtta ccggaatgaa gagttcgggt
    21421 atgacaatat tttttgagaa aaagctgatt acaggttatt ctgtttctcc ggctttaaaa
    21481 caaagaagca gaagatctct cttccgcttc atttgttaaa gatttacatt ttgtatctgt
    21541 aactgatcat aattaagtca tctgacttaa attttatttc aacaatatca ttttttttgt
    21601 ttcaataaaa ttttgcccgt tactgactgc tgttattctg taataataaa ttccggatgc
    21661 cagacttgaa gcattaaaat ttattgaata atatccggct gattttacat cattgaccag
    21721 cgtagctgct tcctttcctg acatatcaaa tattttaatg ctaaccttac cgtcaaatgg
    21781 cagatcatac ctgatagttg tggatggatt aaatggattc ggatagtttt gtgataattt
    21841 aaactgaaca ggaattccta tgcttacttc atttctgaga ttatagtatt caaatgatcc
    21901 gttgaaatct acctgtttca gtctgtaatt atacatgccg gagttaagat ttctgtccgt
    21961 g
//
LOCUS       DAORVQ010000030        21847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_900_length_21847_cov_6.465354, whole genome shotgun sequence.
ACCESSION   DAORVQ010000030 DAORVQ010000000
VERSION     DAORVQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 21847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21847
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_900_length_21847_cov_6.465354"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLX80_04275"
     CDS             <1..558
                     /locus_tag="PLX80_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY20046.1"
                     /translation="EKDFEGAKWVMSPPLREKKDQKALWAGINQGLVQVVGTDHCPFT
                     WEKKLMGEKDFSKIPNGHPAIEHRMELLYSEGVNKAKISLNKYVEVTSTNAAKIFGMF
                     PQKGSIGIGSDADLVIFDPEKKHTLSVKTHHMNVDYSAYEGWEVTGKVETVLLRGKNV
                     VDKGKLNISKGYGKFIKRNKVSKII"
     gene            complement(750..947)
                     /locus_tag="PLX80_04280"
     CDS             complement(750..947)
                     /locus_tag="PLX80_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20047.1"
                     /translation="MPEIQEYKGNKILNLNPESKFTFSFGLAKAKLILEHLDVIKKFV
                     ESEGKDIGDAGSSESKEDQQI"
     gene            complement(978..1775)
                     /locus_tag="PLX80_04285"
     CDS             complement(978..1775)
                     /locus_tag="PLX80_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20048.1"
                     /translation="MKLFYIILFILFFKTSSLYSQEDTTLYFEKDIRVDDYRIILKAN
                     NSDFFSGSISVIDKYNDTVFFREDIFTRFISDTLIDLDINGTKEFVLDLGTGVNAYDF
                     NMLVIFDFKKSDQPLFEIHNAEIIAGTDSIPKIVSHIRFSPLYLGMGYDYSLRYIGNR
                     LVLETDPDSSKVLQSLDINDVDFIENLSGFKSEMDECDKLTNYISFFEQYIFQKMLLN
                     KELEGWKFFDKYYTCPDKESARDDLKESIKHQYKYLSEMDHSFQFER"
     gene            complement(1791..2741)
                     /gene="deoC"
                     /locus_tag="PLX80_04290"
     CDS             complement(1791..2741)
                     /gene="deoC"
                     /locus_tag="PLX80_04290"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQY20049.1"
                     /translation="MKNINSFLNNFNMSVDEIGAKERAASLASRSIKTASKEQALRTA
                     ISMIDLTTLEGKDSDGKVRAMCQKAIHPKPGDDSIPHVAAVCVYPNMIKTAKDAVKNT
                     GIHIASVATAFPSGQYPLKLKLEDVKRCIKDGADEIDMVISRGEFLSGDYNYVYDEIR
                     QIKEECINSSKEYSDKHEVDRDVHLKVILETGELETFDNVRKASFIGMMAGGDFIKTS
                     TGKVQPAATLPVTLVMLEAIRDYYSETGKKIGMKPAGGIKTAKDAIAYLVLLNETLGE
                     EWMTPDLYRLGASSVLNDILMQLDKQKTGFYQSADYYSLD"
     gene            complement(3301..5847)
                     /gene="glgB"
                     /locus_tag="PLX80_04295"
     CDS             complement(3301..5847)
                     /gene="glgB"
                     /locus_tag="PLX80_04295"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQY20050.1"
                     /translation="MMTKKNSKNPGTKKPIPQKLFDKLIKFEEENPFKILGPHLNKSE
                     HTVQINTFLPFAKNGWIKIKGSDKKYEMECISDKGIFQKIFIDRDNIFSYKIIYKNFA
                     DELIEIYDPYAFNIQLSDLDIHLLGEGNHFKTFDKLGARICTVNKVKGVHFCVWAPNA
                     KSVSIVGEFNDWKHGHHPMQRIDGSGYWCLFIPEMKEGTMYKYTVKSKKGDVIFKSDP
                     YAFEAELRPGNASIISSFNKYKWTDGDWVKKRKNSSKSKLSFKTKAISIYEVHPGSWK
                     KDFDNIDFPNEWGYKNYRQLAHELVEYVKEMNYTHIELLPVMEHPLDISWGYQVVNYF
                     APSSRYGSPEDFQYFVDHCHNNGIGVILDWVPSHFPTDEHGLAKFDGTQCYAYEDQLK
                     GFHKDWGTYVFDYGKNEVVNFLICNALFWFEKYHIDGLRVDAVASMLYLDYSRNDGEW
                     IPNIHGGNENLEAIEFLKKLNETVHKKLKGVMMIAEESSSWPGVTRAIHLGGLGFDMK
                     WNMGWMHDILAYFSMDPIYRKFHHGKLTFAIWYAFNENFLLPISHDEVVHLKKSVIEK
                     MPGDEWHKFANLRLMIGFMFSHPGKKLNFMGNDIAQYKEWNSEASLDWEVLDNEMNKK
                     YHDYFRELNRLYKKYPAFHEDDFESSGFQWLDFSDADKCVIAFVRFNKDKSEMLLFTF
                     NMTPVERVDYLFGVPKSGYYREILNSNAEEYGGTGLGNLGGIHSENNPRYEFPDSVKL
                     TLPPLAVNVYKYEKGDVKKIENETEELIEDIEVEITKTETKPATVEISKEVLNEYAEE
                     NSEDIKIESEQQNEILSKEKKDKEVDKQFEMNEDEMNEKIKEVISKIKKF"
     gene            6140..6940
                     /locus_tag="PLX80_04300"
     CDS             6140..6940
                     /locus_tag="PLX80_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY20051.1"
                     /translation="MKNFYVIFSASLLLIFVISFYSFVSESVILNPVSKTDTSECDPN
                     IVYFKQDVLPILQTNCAKSGCHNEESQKHGVILSSYESLMNTVEIEDENDDFDDDSFD
                     DNSKGRNRGGDKFKFILNGSGVNKDRYKNKLEKMLRRNKMPPSPNKKLTDEQKNVIYK
                     WIDQGMLNNTCEVSAADCVFENMTFENDIKPILEENCTGCHGGPKPKNGYDFTNPQKF
                     KEIALTGDVYMAITHSEGVTAMPFKGDKLSDCDIKKIKSWIDNGAVVE"
     gene            complement(6959..8116)
                     /locus_tag="PLX80_04305"
     CDS             complement(6959..8116)
                     /locus_tag="PLX80_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQY20052.1"
                     /translation="MKETDAINLSEIPEDTHNENRAKLAKKYSKINQIISLSETVIFF
                     AITVIFIAGGYSKEVEELAFSFTTNDYAAVLIFFGIFGIIQSVITFPLSFYSDYTLEH
                     KYGLSNQTIPGYFKEKIKGMLIGLLLGIPLLLIFYFIIKNYSESWWLILGAVMFFFSV
                     IIGRLAPTLIMPLFYKFKPLENEEIKQKILSLCEKTGVRIRGIFEFDMSKNTKKANAA
                     FTGIGKSKRIILGDTLLKNFSPEEIETVFAHEMGHYKKRHIMKLMTVSVVMTFAGLYL
                     TSLAYSNSLNTFGFNSVSEIAALPLLFIYLSLFGLITSPISNILSRKFEWEADTFAIE
                     TTRDKGAFISAMEKLADQNLADKTPNKIIEFLFHSHPSIDKRIKFAEDYKL"
     gene            complement(8116..8907)
                     /locus_tag="PLX80_04310"
     CDS             complement(8116..8907)
                     /locus_tag="PLX80_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HQY20053.1"
                     /translation="MNEDNQNNLEENYNSPEGLAPQYQEESLSVSEAMSGVISAPGET
                     FETISVTEKKNYWLMPILIAVLLGLVTSFLFMQDKELTAKTMEKQKEKMMEQFEQNIK
                     DGKMTQEQANNAMETMNPEGTMFKVIGYGFAAIGPFLILLILSVIYFVILKIMKSEAH
                     ITDVMNVVGLSYIIIAIGNLIATVISIVMGDLSSLSLAMLLSEESVGTKVFTMISKVD
                     IFSIWFYTVVAIGITKIGRISMAKSVSFVFGIWLVFYVVIISLFF"
     gene            9143..10381
                     /locus_tag="PLX80_04315"
     CDS             9143..10381
                     /locus_tag="PLX80_04315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY20054.1"
                     /translation="MIDISKKNFNDLIKLNTYLLLIYFIVFLNLNTGVNETVMFSTPD
                     SKDYLEVGNWIFDNSDTEQTINNPVLYPFILKLMLKFSGVYGVWFLQFIFWLLSVNLL
                     FSAIKRLTNKLFAYFGAVIFSLNFSFIALTLHALTDVTVTFVLCWLIYYLTVNYLEIR
                     KWTVFCNVILFFSVLSIIKPVFFPLLIVLLIIVFPVFYLKEFLNSQRKYLLIVALVPV
                     IFQLSLMYAKHNTITISAKGNTTFKTYFFAQSYAEVNNINIDAAVDSSKNLSQDYIYG
                     FMLDNKKIITGNFISNILSQNITGPATFLAYPPGHENMFLFQLMKIENNLFLALHFLF
                     LIPCIIFIFYLRKSSSYKLALYIYLILMFYYLVLITGIAFWQGDRYTISFQPLWILIY
                     LSEVYIFYSVKFNSGGNLVE"
     gene            complement(10419..11000)
                     /gene="nadD"
                     /locus_tag="PLX80_04320"
     CDS             complement(10419..11000)
                     /gene="nadD"
                     /locus_tag="PLX80_04320"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQY20055.1"
                     /translation="MKKYGIFGGTFNPPHIAHSIVAENVRQQLNLDKIIFIPSGNPPL
                     KNSIPAEHRLAMAKLAFGNNKNFEVSEIEFDRPDEKSFTVNTLQMLQKKYINDQVELF
                     LIVGIDNLIDFPKWKEPEKLFELSEVVVINRPNFSDTDSKIEFSSKVKFLTVPFLEIS
                     SSSIRQLVSDNRSIHYLVNPDVEKYIADHKLYI"
     gene            complement(11007..12110)
                     /locus_tag="PLX80_04325"
     CDS             complement(11007..12110)
                     /locus_tag="PLX80_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronosyltransferase"
                     /protein_id="HQY20056.1"
                     /translation="MPKAVLITSHFLNSKRKAGFHNIAYSLLEQGYTVLFLTGNASYI
                     HHLKKDYRASLINKTPLNKLLKEKDDLYQFIRFTTLHPVNFRSDLINNIFLPSVKKYS
                     GALSGFDDLKIFISESSLFIFESFPGLLWFEYFKSLNNKAKYVYRVSDDLRQLRKHPY
                     LIKHEEKILDKFDLVSVPSKYIYDVLKGKGNVKLHHHGINKEFFDKVDTSPYGNNESF
                     KFVFTGNAYLDYEFLETASDNFPKDEFHILGPFKDKLYKKNIKFHGEIKFDDTVPFIK
                     FADAGLHTLIYTGGAESFSDSLKVIQYTYCRLPVIAPEFIKSNRENFIYYKPFDIESI
                     KSSIYSAKKFDKEKIIGSEISDWKDLTLKLISE"
     gene            complement(12112..14073)
                     /gene="asnB"
                     /locus_tag="PLX80_04330"
     CDS             complement(12112..14073)
                     /gene="asnB"
                     /locus_tag="PLX80_04330"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQY20057.1"
                     /translation="MCGICGIVTSSENVDFNIVKSMNDTIAHRGPDDEGYLIYNSETQ
                     EVLDIKRDLAGSEKLSQKNYNLLIGHRRLSILDVSDQGHQPFKDPSGRYFLILNGEIY
                     NYIELKKILVEKGYKFISTSDTEVLLYLYLEYKEKCLEMLNGMWAFVILDTVSNTLFG
                     SRDRFGVKPFYYYLDNKSFIFSSEIKGLIKAPQISREINKEAAFDYLTNGRLSDNVST
                     LFKNVIELEPSSYFSLDLKNFKFNTGSYYSLKFNSSWEKFDKIKSENYVSEIREKIFN
                     AVNLRLRSDVKVGSCLSGGVDSSSIVCVIDELLKKNKISQVGDIQKVFTSCYPDSTID
                     ETGWAALVADSTASEWFKTFPTSAGLMEDLEKLVYVQDVPFGSTTIYSQYKVMQLASE
                     NGVKVLLDGQGGDELFTGYTYFMRPFYNEMLKNFEFSSLINEYKSIENSPISKRDVIK
                     ALTMTTAKLVMPATIKRKLFSMKVRENYLLNKNFYKKYEPRLDLGKLIEITDLNHALH
                     DQFTKTSLKELLKYEDRNSMNFSIEARTPFADDSDLVDYVFSIPSSYKIHNGWTKYLL
                     RESMKGIIPDAIRNRTDKLGFATPEYNWLSSIKDNLKDYFTDDLDEYMETSKIVNNWN
                     SIFDGQSKTGFTNIWRFINFAVWKKVYQM"
     gene            complement(14077..15225)
                     /locus_tag="PLX80_04335"
     CDS             complement(14077..15225)
                     /locus_tag="PLX80_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20058.1"
                     /translation="MTNSAVKKKVLIISLTNQDSDPRILRQIDFLKGSFDVYCCGIFN
                     SSISSDKFYKADLTKNSIFIKIVIGTLKLLRLFSPAEKFFFNVKLKIHEYENSPLFDL
                     IIANDLDTVPVAFNFFRGKKVLADLHEFTQSEFEDKFYFKYIHKDYVIYECKKYFPKL
                     NAVTTVCNSIADEYKKQYSVDPVVITNAAEYENLSPVPVVNKIKIIHHGAAIRSRRIE
                     LMIETAKLLDDRFTLDLMLIPNEPDYYSYLKEMISGLDKVRMIAPVDYKNIVSYCNTY
                     DIGMFILPPVNLNYKYALPNKFFEFVMSRLAVITGPSIEMSYYINMYELGISTDSFNP
                     SQIAKEINKLSNEQIQKFKQNSDKSAYELSSETNREIFLKLVNNLTEK"
     gene            complement(15278..16474)
                     /locus_tag="PLX80_04340"
     CDS             complement(15278..16474)
                     /locus_tag="PLX80_04340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20059.1"
                     /translation="MNIYIIPSWYPDNENPLDGIFVREQAEALAEFHEDINFVVSICS
                     NNYLSISRPDIYFKTLKKFKSSAKIEVSLRKNLIELYNSALTWTPKLGGEINNIVKAH
                     ESNFLKARSEFGEIDLIHAHVSFPGGFAAMELKKKYNIPYVITEHMGPFPFEKFLENG
                     NISSKISEPLRNADRVIAVSSSLSKKIESFKIPTPVIIPNLVNENIFTPSEAENKSDD
                     HKIKFLTVCSFVEQKGIKELMNGIMKAVQHSCNFEITIAGSGPMEQYIENFIKENKLN
                     GIITLIRNPERNNIAELFRESDTFILPSRHESFGIVYLEAMSCGIPVIATDCGGPSDF
                     IRDFNGILIPVNDADKISEALIYISDNVSKYSKDVIREFVLDNYSRKAVTDKIISLYK
                     EVLKIY"
     gene            complement(16484..17800)
                     /locus_tag="PLX80_04345"
     CDS             complement(16484..17800)
                     /locus_tag="PLX80_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY20060.1"
                     /translation="MGIINEIRQKQFSKNVSILASGTIIVQIISFLLSPVLSRLYTPE
                     DYGLFAVFTSIISILTVMCCLRYELAIVLPKSDSEALSLLRLCIYLISILSFLVLVIV
                     LIFKGYIAELLGTATIENWLFLIAPVLFLTGSVQALTYWFNRKKEYKIISGSRVSQSS
                     VNSTSSLILGLLKYNSFGLIISSAISQLLPAVFLYKKSDVRIFNTNKNESNIPELRSV
                     SLKYKEFPLFNLPSAFFDTLSVNSLIFILTYLFSESVTGSYSFALRILSIPGIVIGAS
                     IGQVFYQKISEAFYNKQKITELIFKSWKVLFLLSIIPVLILFFFGEDIFRFVFGTKWT
                     EAGVIAKYLCILTMFTFISSPTSSAMIILKKQKEAMILNIFAFIYRPLSIVAGFYLGN
                     FVLGIALYVIFEIIQIITYNTIMIKASKKADLEFSTENVNGNNNNN"
     gene            complement(17931..18929)
                     /locus_tag="PLX80_04350"
     CDS             complement(17931..18929)
                     /locus_tag="PLX80_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HQY20061.1"
                     /translation="MGLFLAFASLIAAVVPMFIYLIIIWYLDRYEREPFWFVLLNFFW
                     GATGAILLGIIGSLIFQIPLNELIRAVSDDNPSELMNLSGAVITAPLIEETTKGIFLI
                     IVSFSKRFDGGVDGAVLGGAIGLGFGMSENFLYFMSYGTTPASWLMLVIIRTLFSAVM
                     HCLSTATFGAFLGYAKFKPMILKFILVPMGFFLAVFLHFSWNLSVSFEDSTIFGFLFL
                     ILYLFVTFSIFQISVYFEGKTILKELQDEVSLGFIPPDHIHHLPYVMRRFKYGWCPVG
                     VDQKNYVKTATTLALRKYQHRNVSGMKKQNYQRDIDNLRYKIQMMFYNANLHNYNT"
     gene            complement(19005..19532)
                     /locus_tag="PLX80_04355"
     CDS             complement(19005..19532)
                     /locus_tag="PLX80_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HQY20062.1"
                     /translation="MKKFQISFADPDSTGVSHSNQELTLLSDNTAIVNGKEIEFDSKW
                     LNPNVLILRINNKNYYITAEENTDENFTEINIDSLTHRVTCKSEFDILKEQFAGNKSD
                     DKSKSDILSPMPGIIKKMNVTEGQEVKKGDVILVLEAMKMENEIKAKKDSIIKKICVE
                     EMCSVEKNELLIMLE"
     gene            complement(19529..21034)
                     /locus_tag="PLX80_04360"
     CDS             complement(19529..21034)
                     /locus_tag="PLX80_04360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQY20063.1"
                     /translation="MFKKILIANRGEIAVRIISTAKEMGIKTVAVYSDFDRYSLFTKL
                     ADEAYHLGESPSSKSYLLADKIIDIAKKTGAEAIHPGYGFLSERGFFAKMCKDNGIRF
                     IGPSAESIDLLGSKTDAKGLALKNDVPVVPGTDKPLQDMSKIKSIASEIGYPVLIKAS
                     AGGGGKGMRVVNEEADIESSLRMAQSEALSSFGDDSVFIEKYIADPRHIEFQILADEH
                     GNVIHLGERECSMQRRHQKIIEETPSVIMDENLRHEMGECAKRIVKAAGYSNAGTVEF
                     IVDNKNNYYFLEVNTRLQVEHPITEIRTGLDLVREQLKIANGEKLSYKQEDIKFTGHT
                     IECRICAEDSYNNFMPSTGKITYMKKFLGCGMREDTGIEQDDEISIYYDPMVSKLISY
                     GANREIAIDKMKRALKEYKIVGIKTNIGFLLSLLESKEYIEGNYHTQFIEKEFQKRIN
                     GNENSDEEINIAVCLASVLLKEKKSLVNGKFKISSNGAFSNWIKKRQDSYR"
     gene            21339..21701
                     /locus_tag="PLX80_04365"
     CDS             21339..21701
                     /locus_tag="PLX80_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY20064.1"
                     /translation="MQRIDLINKVADSIDRLDGKAFSEFITEDGSFKYANNEPVKGRK
                     NIEEYVSAFFKFVKSGKHKVIRIWEDGDSLVWQGEVTYTRQDDKVMTFPFVNVFKMDG
                     DLISDYQIHIDNTPLFGE"
BASE COUNT         6990 a         3961 c         3341 g         7555 t
ORIGIN      
        1 gaaaaagatt ttgaaggtgc aaaatgggta atgtctccgc cgctcagaga aaagaaagat
       61 caaaaagcct tgtgggcagg catcaatcag ggacttgttc aggtcgttgg tacagaccac
      121 tgtcctttta cctgggagaa aaaacttatg ggagaaaaag atttttcgaa aattccaaac
      181 ggacatcctg ccatcgagca tcgcatggaa ttactttatt cagaaggtgt aaataaagct
      241 aagatcagtc ttaacaaata tgtagaagta acttcgacaa acgctgcaaa aattttcggc
      301 atgttcccgc aaaaaggttc tattggaatt ggaagtgacg cagatcttgt catctttgat
      361 cccgaaaaga aacatactct ttcagtaaag actcatcata tgaatgtaga ttattcagct
      421 tatgaaggat gggaagttac cggtaaagtt gaaactgttt tgcttcgcgg taagaatgtc
      481 gttgataaag gaaaactcaa catcagcaaa gggtacggaa aatttatcaa aagaaataaa
      541 gtatcaaaga tcatttgatt ttatttattg aatttactcg ctcctaaatt tgcctgccca
      601 aaaaccgctc gttaccatac tccgtttggt aacggacttt gttttgaaac tccgtttcaa
      661 acttcactca ttcacaatgt ctctaaataa aatcattaac ataaattaaa attttgagcc
      721 ttcgagtctt cgtggcaagc ccaacatttt cagatctgct gatcttcctt actctcactg
      781 cttcctgcat caccaatatc ttttccctca gactctacaa actttttaat cacatctaaa
      841 tgctcaagta tcagcttggc ttttgcaaga ccgaatgaaa aagtaaattt actttcggga
      901 ttcagattga gaattttatt tcctttgtat tcctgtatct caggcatgat tctatattta
      961 aattattaaa aaatattcta tctttcaaat tgaaaactat gatccatttc tgaaagatac
     1021 ttgtattgat gctttattga ttctttcaga tcatctcttg cactttcttt atccggacat
     1081 gtataatatt tatcaaaaaa tttccatccc tccagctctt tattcaaaag cattttctga
     1141 aaaatatact gttcaaaaaa tgaaatgtaa ttggttaatt tgtcacattc atccatctca
     1201 cttttaaagc cgctaagatt ttcaataaaa tcgacatcgt ttatgtccag actctgaagt
     1261 actttacttg aatccggatc tgtctcaagg actaatctgt ttccgatata ccttaaagaa
     1321 taatcataac ccatgccaag ataaagcgga ctgaatctga tgtgagatac aattttcgga
     1381 atgctgtcag tgccggctat tatttcagca ttatgaattt caaaaagagg ctgatcactt
     1441 tttttgaaat caaatataac aagcatatta aaatcatacg cattcactcc tgttccaaga
     1501 tccagaacaa actcttttgt tccattaata tccagatcta tcaaagtatc agaaataaat
     1561 cttgtaaaaa tatcttctct gaaaaatacg gtatcgttat atttatcaat tacagaaatg
     1621 cttcccgaaa agaaatcaga attatttgct ttcagtatta ttctgtaatc gtcaacacga
     1681 atatcttttt caaaatataa tgtagtatct tcctgactat acaatgatga cgttttgaaa
     1741 aaaagtataa agagaattat ataaaataat ttcaatgtac ttaaccttat ttaatctaaa
     1801 gagtaataat ctgcgctctg ataaaatcct gtcttttgtt tatcaagctg catcagaata
     1861 tcattcagca ctgaactggc gccgagtctg taaagatcag gtgtcatcca ttcttctcca
     1921 agtgtctcat tcaatagtac cagatatgca atcgcatctt ttgcagtctt aatcccgccg
     1981 gcaggtttca ttccgatctt ttttcccgtc tcagaataat aatccctgat cgcttcgagc
     2041 attactaatg tcacaggcag agtggctgca ggctgaactt tcccggtaga ggtctttata
     2101 aaatctccgc ctgccatcat tccaataaag ctcgcctttc ttacattgtc aaaagtttcc
     2161 agttcgcctg tttcaagaat tactttcaga tgaacatcac gatccacttc gtgcttatca
     2221 ctgtattcct tacttgaatt aatacattcc tcctttatct gtcttatctc atcatataca
     2281 taattataat cacccgacaa aaactcgcct ctcgaaatca ccatatcgat ctcatctgct
     2341 ccgtccttta tacaacgctt aacatcttct aatttaagct tgagcggata ttgacctgaa
     2401 ggaaatgcag ttgctactga tgcaatgtga attcctgtat tttttacagc gtccttcgca
     2461 gtttttatca tattaggata gacgcaaact gcagctacat gcggaataga gtcatcaccg
     2521 ggttttggat gaatggcttt ctgacacatt gcgcgaactt tcccatcaga gtctttacct
     2581 tccagcgttg taagatcgat catcgaaatc gccgtcctga gtgcctgctc cttactcgcc
     2641 gttttaatgc tcctcgatgc aagtgaagct gcgcgctcct ttgcgcctat ctcatcaaca
     2701 gacatattga aattattcag aaatgagttt atatttttca ttttgtattt agtgttaatt
     2761 tctttaaatg gaaatttaca agatcaaact tttataaatt ctgatttcta taatattaat
     2821 tttcgtattc aataaaaatg atttaattgt ttgcttaaaa tatttattca cattagcata
     2881 aaataagcat tatataaatg aaacaaacag ttagatatat atttaaaaag taatttgttt
     2941 ttaaaaagaa atctccgctg tttaaatttg attaaaaact tatataaaat taccgcctct
     3001 gataaattgc cgtaaagaaa ttctgagcta tggcgttaag caaaaatata ctgtatgacg
     3061 aatccgttag tgttcttcta acgggtgaag agtttatatt tttgtagcca agcgaaagaa
     3121 tttccggcaa tttatcacca gcggtgattt ttttggttct ttggagcctg cccctttgtg
     3181 cttccaaagg gggatcgctc aaaaagaaca gtagaaaatg taaatattta attttatgct
     3241 taatcttttg gaggagtatt atttaattaa atttattttt attaaaatga aactaatcat
     3301 tcaaaacttt ttaatcttac taataacctc ttttatcttt tcattcatct catcttcatt
     3361 catttcaaat tgtttatcta cttccttatc ttttttctcc ttagataaaa tttcattttg
     3421 ttgctcagat tcaattttta tatcttcaga attttcctcc gcatactcat ttaaaacttc
     3481 ttttgaaatt tcaactgttg ccggtttagt ttctgtttta gtaatttcaa cttcaatgtc
     3541 ttcaattaat tcttcagtct cattttcaat tttcttaaca tcaccttttt catatttata
     3601 aacattcacc gccaatggtg gaagtgtcag ttttactgaa tcaggaaatt cataccttgg
     3661 attattttca gaatgaattc cacccaaatt acccagtcct gttccgccat attcctctgc
     3721 attgctgtta agaatttccc tgtagtaccc ggactttggc actccaaata aataatcaac
     3781 acgctccaca ggtgtcatat taaaagtaaa gagcagcatc tcacttttat ccttattaaa
     3841 tcttacaaat gcgatcacac atttatccgc atcagaaaaa tccagccact gaaatcccga
     3901 actttcaaaa tcatcttcat gaaatgccgg atacttttta taaagtctgt tcagttcacg
     3961 aaaataatca tgatactttt tattcatttc attgtcgagc acttcccagt caagtgaagc
     4021 ttcagaattc cattctttat actgagcaat atcatttccc atgaagttaa gtttcttccc
     4081 cggatgactg aacataaatc caatcatcag tctgagattc gcaaacttat gccattcatc
     4141 accgggcatt ttttctatta cagatttctt cagatggacg acttcatcat gagaaatagg
     4201 cagcagaaaa ttttcattaa aagcatacca tattgcaaat gtaagtttac catgatgaaa
     4261 ttttctgtag atcggatcca ttgaaaaata tgcaagtata tcatgcatcc agcccatatt
     4321 ccatttcatg tcaaatccca gtccgccaag atgtatcgct ctcgtcaccc ccggccatga
     4381 agatgattct tcagcgatca tcatcacgcc ttttaatttt ttatgaactg tttcgtttag
     4441 cttttttaga aattctattg cctcgagatt ctcattccct ccgtgaatat ttgggatcca
     4501 ttctccatca tttcgggaat aatccaggta cagcatagat gctacagcgt cgactcttaa
     4561 tccgtctatg tgatattttt caaaccaaaa caaagcatta caaatcaaaa aatttacaac
     4621 ttcatttttg ccataatcaa aaacatacgt tccccagtct ttgtgaaatc cttttaactg
     4681 atcttcatat gcataacact gtgtgccgtc aaactttgca agaccatgtt catcagtcgg
     4741 aaaatgtgac ggtacccagt cgagtataac acctatcccg ttattgtggc aatgatctac
     4801 aaagtactga aaatcctcag gacttccgta acgactcgat ggcgcaaaat aatttacaac
     4861 ctgatagccc catgaaatat ccagcggatg ttccatcaca ggaagtaatt caatgtgtgt
     4921 gtaattcatt tcctttacat attccacaag ctcatgtgca agctgtctgt aatttttata
     4981 accccattca tttggaaaat caatattgtc aaaatctttt ttccatgaac ccggatggac
     5041 ttcgtaaatt gaaattgctt tggttttaaa tgatagtttt gatttagaag aattttttct
     5101 tttcttcacc caatcaccgt ctgtccattt atacttatta aatgaagata taatcgaagc
     5161 attgccgggt ctgagctcag cttcaaaagc gtaaggatca gatttaaaaa tcacatctcc
     5221 ttttttagat ttgactgtgt atttatacat cgtgccttct ttcatttcag gaatgaaaag
     5281 acaccagtat ccagagccgt caattctctg catcggatgg tgaccgtgtt tccagtcatt
     5341 gaattcacct acaatgctga cactctttgc attcggtgcc catacacaaa aatgaactcc
     5401 ctttacttta ttaactgtgc agattcttgc accgagttta tcaaatgttt taaaatgatt
     5461 tccctctcct aaaagatgaa tatcaagatc acttaactga atattaaaag cataaggatc
     5521 atatatttca atcagctcgt cagcaaaatt cttgtaaatt attttatagg aaaaaatatt
     5581 gtcccgatct ataaatattt tttgaaagat ccctttatca gaaatacatt ccatttcata
     5641 cttcttatca cttcctttta tttttatcca tccatttttt gcaaatggaa gaaaagtatt
     5701 tatttgaaca gtatgttcag atttatttaa atgcggacca agaattttaa aaggattttc
     5761 ctcttcgaat ttgatcagtt tatcaaataa tttctgagga attggttttt tcgtgcccgg
     5821 atttttagag ttctttttag tcatcatttt atcaatatgc taaaatctgc aaaagttttt
     5881 acaaattaaa ttcaatctgt aaattttaat gtgcagtttg aaagctgtat taaaaatttt
     5941 gttaatgagt tttaatacct taagctgtaa atcagaacca atgtttatat tatggaaaat
     6001 ttattttcat aaaatttcac tttctgaagt ttgtttactt cgatactaca attgaaacat
     6061 atataaactt gctaaatcct aattcatttt ctattttatg ctttattttt aaatctaaaa
     6121 actcaataat atctgtacaa tgaaaaactt ctatgtcata ttctctgctt cacttttgct
     6181 aatatttgtg ataagctttt attcttttgt ttcggagtca gttattttaa atcctgtttc
     6241 aaaaactgat acatctgagt gtgatccaaa tattgtatat ttcaaacagg atgttcttcc
     6301 aattcttcaa accaactgtg caaaatcagg atgtcacaat gaagaatctc aaaagcatgg
     6361 cgttatcctt tcaagttatg aaagtttaat gaatactgta gaaatagaag atgaaaatga
     6421 tgacttcgat gacgatagtt ttgatgacaa tagcaaaggc aggaacagag gcggtgataa
     6481 atttaagttt atattaaacg gaagcggtgt aaacaaagac agatataaaa acaaactcga
     6541 gaaaatgctt cgtcgaaata aaatgccgcc atctcctaac aagaaattaa ctgacgaaca
     6601 aaaaaatgtg atctataaat ggatagatca gggaatgttg aataacactt gtgaagtttc
     6661 cgcagctgat tgtgtatttg aaaatatgac attcgaaaat gatatcaaac ctattcttga
     6721 ggaaaattgt accggctgtc acgggggacc caaacctaaa aatggttatg attttacaaa
     6781 tcctcaaaag tttaaagaaa tagcacttac aggagacgta tacatggcaa ttacacattc
     6841 agaaggagta acagcaatgc cttttaaagg tgataaactc tctgactgcg atataaaaaa
     6901 aattaaatca tggattgata atggagctgt agtagaatga tatttaaaac tgaatttttt
     6961 agagtttata atcctctgca aactttattc tcttatcaat tgatggatgc gaatgaaata
     7021 aaaattcaat tattttatta ggcgtcttat cggcaagatt ctgatctgca agtttttcca
     7081 tggcagaaat gaaagctcct ttgtcacgtg ttgtttctat ggcaaatgtg tccgcttccc
     7141 actcgaattt tctcgaaaga atatttgata tcggtgaagt gatcagacca aacaaactta
     7201 aataaataaa cagtaaaggt aaagcagcaa tctctgagac agaattgaat ccaaaagtat
     7261 taagtgaatt cgaatatgca agagatgtca gatacaaccc cgcaaaagtc atgactacag
     7321 atactgtcat tagtttcata atatgccttt ttttatagtg acccatctca tgagcaaata
     7381 ctgtctcaat ttcttcaggt gaaaaatttt tcaatagtgt atcgccgagt atgatgcgtt
     7441 ttgattttcc aatacctgtg aatgctgcat ttgctttctt tgtattcttg ctcatgtcaa
     7501 attcgaatat cccccggatt cttacacctg ttttttcaca tagtgataat atcttctgct
     7561 ttatctcttc attctcaagt ggtttgaatt tataaaacag cggcatgatg agtgtcggag
     7621 caagtcttcc gattattaca gaaaaaaaga acatcactgc tccgagtata agccaccagc
     7681 tttcactata attttttatg ataaaataaa atatcagcaa cagtggaatt ccaagcagca
     7741 gtcctattaa cattcctttt attttttcct taaaatatcc aggaatggtc tgattcgata
     7801 atccgtattt atgctcaagt gtgtagtcag aataaaaact taatggaaat gttataacag
     7861 attgtataat accgaatatt ccaaaaaaaa tcagaactgc tgcgtaatca tttgtagtaa
     7921 aactaaatgc taattcttcc acctcttttg aatatcctcc tgcaataaat attactgtta
     7981 tagcaaaaaa gatcactgtc tcggacaatg aaattatctg atttattttt gaatattttt
     8041 ttgcaagctt tgcacggttt tcattgtgtg tatcttcagg aatctcagat aaatttattg
     8101 catcagtttc tttcatcaga agaataatga aattattact acataaaata ctaaccaaat
     8161 tccaaatacg aatgaaacag attttgccat actgatcctt ccgatctttg taataccaat
     8221 cgccactact gtataaaacc agatcgagaa tatgtcaact tttgagatca tagtaaagac
     8281 tttagttcca acagattctt cacttagcaa catagccaga ctcagactgc tcagatcacc
     8341 cattacaata gatataactg ttgcaattag atttccaatc gcaatgatta tatatgaaag
     8401 tccaactaca ttcataacat cagtaatgtg agcttcggat ttcattatct ttaatattac
     8461 aaaatagatt acactaagaa tcagaagaat taaaaacggt ccgatcgctg caaatccata
     8521 tccaataact ttaaacattg taccttcagg attcattgtc tccattgcat tatttgcctg
     8581 ttcctgagtc atctttccat ctttgatatt ttgttcaaac tgttccatca ttttctcttt
     8641 ctgcttttcc attgtttttg cagtcagctc tttatcctgc ataaatagaa atgaagttac
     8701 cagcccaagt aatacagcaa taagtatcgg catcagccag taattttttt tctcagtgac
     8761 agatattgtt tcgaatgttt cacccggtgc tgaaataact cctgacattg cttctgaaac
     8821 tgaaagagat tcctcctgat attgaggtgc taatccttcc ggagaattgt aattttcctc
     8881 taaattgttt tgattatctt cattcatttt atagggtttt taaatttatt tcgaaaatga
     8941 taaaatattt taatatgatt tgatacaaat atttatatgg ggatttgaat tttttaaaat
     9001 tactaaatat acttttaaaa agttattact aatttatgta aatttttgta agttctgctc
     9061 aattaacatt tgttgtatta actttattta ctcaagtacc gacaattact acaatcctga
     9121 tgattctcaa aacttccaaa taatgataga tatttcaaag aaaaatttca atgatctgat
     9181 taagttgaat acttaccttc tgctgatcta ttttatagtt tttcttaatc tgaatacagg
     9241 agttaacgag accgtaatgt tttcgactcc ggattctaaa gattatctgg aagtcggtaa
     9301 ctggatattt gacaactctg atactgaaca gactataaat aatcccgtgc tttatccgtt
     9361 cattctaaaa ctaatgctga agttttcagg agtttacgga gtatggtttt tgcagtttat
     9421 attttggctg ctctctgtga acttactgtt ttcagctatc aaacgactta caaacaaact
     9481 atttgcatat tttggtgctg taattttttc actgaatttt tctttcattg ctcttacact
     9541 gcatgcactt actgatgtga cagtaacttt tgtattatgc tggctgattt attatttgac
     9601 tgtaaattat ttggagattc ggaaatggac tgtattctgt aatgtgatac tttttttttc
     9661 agtcttgtca attattaaac ccgtgttttt tcctttgcta atagtactct taataattgt
     9721 atttccggta ttttatttaa aagaattttt aaacagtcaa aggaaatatt tactcatcgt
     9781 tgcacttgta ccggtaattt ttcaattatc cctaatgtat gctaaacata atactattac
     9841 aatttcagct aaaggaaata ctacctttaa aacttacttc tttgctcaaa gttatgctga
     9901 agtaaataat ataaatattg atgcagcagt ggacagctca aaaaatttat cacaggatta
     9961 catatatgga tttatgctgg acaacaaaaa aatcattacc ggtaatttta tcagtaacat
    10021 attatctcaa aatatcacag gacctgcaac ttttcttgct tatccacccg gtcatgaaaa
    10081 tatgttttta tttcaattaa tgaaaattga aaataactta ttcttagcct tacatttttt
    10141 atttttaatt ccctgtatca tttttatttt ttatcttaga aaatccagtt catataaact
    10201 tgccctgtac atatacttaa ttttaatgtt ttattatctg gtattaataa cagggatagc
    10261 attctggcag ggtgacagat atactatatc ttttcagcca ctctggatat taatttatct
    10321 atctgaggtg tatatttttt attctgtgaa gtttaattca ggtgggaatt tagtagagtg
    10381 atttgaaaat agattctgat tgttaattcc ccaactcttt aaatatataa cttatgatca
    10441 gcaatatatt tttctacatc cggatttact aaataatgaa tagatctgtt gtctgaaaca
    10501 agctgcctga tactggatga tgaaatttca agaaacggta ctgtaaggaa ttttactttt
    10561 gatgaaaact caattttaga atccgtatca gaaaaattcg gacggttaat cacaactact
    10621 tcagaaagtt caaataactt ttcaggttct ttccatttcg gaaaatcaat taaattatca
    10681 attccaacta tcaaaaataa ttcaacctga tcatttatat attttttctg cagcatctga
    10741 agagtattca ctgtaaatga tttttcatca ggcctgtcaa attcaatttc actgacttca
    10801 aaattcttat tatttccgaa tgcaagttta gccatagcaa gtctgtgctc agccggtata
    10861 gaatttttta aaggcggatt gccagaaggt atgaaaataa ttttatcaag gtttaactgc
    10921 tgcctgacat tttctgctac tattgaatga gctatgtgag gcggattaaa tgtacctccg
    10981 aagattccgt attttttcat gatctcttat tctgagatta atttaagtgt taagtctttc
    11041 cagtctgaaa tttccgaacc aattattttt tctttgtcaa atttttttgc tgagtatatc
    11101 gaactcttta tgctttcaat gtcaaacggt ttgtagtaaa tgaaattttc cctgttgctt
    11161 tttataaact ccggagctat caccggaagc cggcagtaag tatattgtat tacttttaaa
    11221 ctgtcggaaa atgattctgc gccaccggta tatataagcg tatgcagacc cgcatctgca
    11281 aattttatga atggtactgt atcatcaaat tttatctctc catgaaattt aatattcttc
    11341 ttatataatt tatccttaaa aggtccgagt atgtgaaatt catctttggg aaaattatca
    11401 gaagccgttt caagaaattc ataatcaaga tatgcattcc cggtaaacac gaatttaaaa
    11461 gattcattat ttccataagg actggtatca actttatcaa aaaattcttt attaattcca
    11521 tgatgatgaa gtttaacatt tcctttccct tttaatacat cataaatata ttttgacgga
    11581 actgaaacta aatcgaattt atccaatatt ttctcttcat gtttaattaa atatggatgt
    11641 ttgcgcaatt gtctcagatc atcagaaact ctgtaaacat attttgcctt attgttcaga
    11701 cttttaaaat attcgaacca tagcagtccg ggaaaactct cgaaaataaa taatgatgat
    11761 tcactaataa aaatttttaa atcatcaaat ccggataatg cacctgaata tttttttaca
    11821 gagggaagaa aaatattgtt tatcagatca gatctgaaat tcaccggatg taatgttgtg
    11881 aatcgtatga actgatacag atcatctttt tcctttaaca atttattcag aggagtcttg
    11941 tttatcagtg aagccctgta atcttttttt aaatgatgaa tgtaacttgc attacctgtc
    12001 aggaaaagca cagtatatcc ctgctcaaga agtgaataag caatgttgtg aaaaccggct
    12061 ttcctcttgg agtttaaaaa atgagatgtg atcaatactg cttttggcat attacatttg
    12121 gtaaactttc ttccagacag caaaatttat gaaccgccag atgttggtaa atcctgtctt
    12181 tgactgtccg tcaaaaatac tgttccagtt gtttactatc ttagatgtct ccatgtactc
    12241 atccagatca tctgtaaaat aatctttcag attgtctttt atagaagaca accagttata
    12301 ttcaggagtt gcaaagccta atttatcagt tctgtttctg atcgcatcag gaattattcc
    12361 tttcatggat tccctcagca gatattttgt ccagccatta tggatcttat atgaagatgg
    12421 aatgctgaat acatagtcaa cgagatcaga atcatccgca aatggtgttc tggcttcaat
    12481 agaaaaattc atagaatttc tgtcttcata tttcagaagt tcctttaagc ttgtctttgt
    12541 aaactggtca tgcaatgcat gattcagatc agtgatctca atgagttttc caagatcaag
    12601 cctgggttca tattttttat aaaaattttt attcagcaga taattttccc tgactttcat
    12661 gctgaataat tttctcttta tggtagcagg catgacaagt tttgcagttg tcatagtaag
    12721 agcttttatt acatctcttt tactgatcgg tgaattttca atactcttat attcattaat
    12781 aagtgaagag aattcaaaat tctttaacat ctcattataa aaaggtctca taaaataagt
    12841 gtaacctgtg aagagttcat caccgccctg accgtccagc aaaactttca ctccgttttc
    12901 agaagcaagc tgcatgactt tatactgaga ataaatagta gtcgatccga atggaacatc
    12961 ctgaacatat accagttttt ccagatcttc cataagtccc gctgatgtag ggaaagtttt
    13021 aaaccattcg cttgcagtag aatctgcaac gagcgctgcc cagcctgttt catcaattgt
    13081 agaatcagga tagcatgaag tgaaaacttt ctggatatca ccaacctgtg aaattttatt
    13141 ttttttcaga agctcatcaa tcacacaaac tatcgatgaa ctgtcaactc cgccgctaag
    13201 acatgaacct actttcacat ctgaccttaa tctcagattg acagcattaa aaatcttctc
    13261 tctgatttca ctaacataat tctctgattt tattttatca aacttttccc agcttgagtt
    13321 aaatttcaaa ctgtaatagc ttccggtatt aaatttaaaa tttttcagat caagactgaa
    13381 gtaagaggaa ggttctaatt caatgacatt tttaaaaagt gtagatacat tatccgagag
    13441 cctgccattt gtaagataat caaacgctgc ttctttgttt atttctctgc tgatctgagg
    13501 agctttgatc agtcccttaa tttcagaaga aaaaataaaa cttttgttat ccagataata
    13561 ataaaaaggt ttaacaccga atctgtcacg tgagccaaac agtgtatttg aaactgtatc
    13621 aagtatcaca aatgcccaca taccattcag catctccaga catttctctt tgtattcgag
    13681 atatagataa agtaaaactt cagtatcaga agtggaaata aatttataac ctttttcaac
    13741 aaggatcttt tttagctcaa tataattata tatctcaccg ttgagaataa gaaaatatct
    13801 tccggatgga tctttaaaag gctgatgtcc ctgatccgat acgtcaagaa tagaaagtcg
    13861 tctgtgaccg atcagcagat tataattttt ttgagaaagt ttttctgaac ctgcgagatc
    13921 tcttttgata tcaagaactt cctgtgtctc agaattatat attagataac cttcatcgtc
    13981 aggacctctg tgagcaatcg tatcattcat gctctttaca atattgaaat caacattttc
    14041 tgaagacgta actataccgc aaattccgca catattttat ttttctgtta aattgtttac
    14101 aagttttaaa aaaatttctc tgttcgtttc agaagaaagt tcataagcac ttttatctga
    14161 attctgtttg aatttttgta tctgctcatt gcttaattta tttatttctt ttgcaatttg
    14221 cgacggatta aaagagtcag ttgaaattcc cagttcatac atatttatat aataagacat
    14281 ctctatcgaa ggaccggtaa tcacagcaag tcgcgacatt acaaattcaa aaaatttatt
    14341 tggtagtgca tatttgtaat tcagatttac aggcggcaga ataaacatcc cgatatcgta
    14401 agtgttgcaa tagctaacaa tatttttgta atcaaccgga gcgatcatcc taactttatc
    14461 caaccctgaa atcatttctt taagataaga ataatagtca ggttcatttg gaattaacat
    14521 aagatctaaa gtaaatctgt cgtccagcaa cttagctgtt tcgatcataa gttcgatcct
    14581 tctggatcgg atagctgcgc cgtgatgaat gatcttaatt ttatttacta caggtacagg
    14641 acttagattt tcatattcag cagcatttgt aataaccaca ggatctacag aatattgttt
    14701 tttatattca tctgcaatgc tgttacatac agtggtaact gcatttaatt tcggaaaata
    14761 ttttttacat tcatatatca cataatcttt atgaatgtat ttgaaataaa atttgtcctc
    14821 aaattcagac tgcgtgaatt catgcagatc cgccaagact tttttacctc tgaaaaaatt
    14881 aaatgctaca ggtacagtat ccagatcatt tgcaattatc agatcaaaca aaggagaatt
    14941 ttcatattca tgtattttca gttttacatt aaaaaaaaat ttctctgccg gtgaaaataa
    15001 tcttaaaagt ttaagagtac ctataactat ctttataaaa atggaattct tagtgagatc
    15061 agctttatag aacttatcag aacttatact gctgttaaat atcccacaac agtaaacatc
    15121 aaaactaccc ttcagaaaat cgatctgtct caatattctc ggatcactgt cctgattggt
    15181 cagtgaaata attaatactt tttttttaac tgctgagttt gtcaaaaaga aatttgaatt
    15241 ttattattat aggtctgcca tgtaaaataa agtaacatca atatattttt aaaacctctt
    15301 tatataaaga tataatttta tcagttacag ctttgcgtga ataattatcc agtacaaact
    15361 ctctgatcac atcttttgag tacttagaaa cattatccga aatataaatc agcgcttctg
    15421 atattttatc cgcatcattt acagggatca gtataccgtt aaagtctctg ataaaatctg
    15481 aaggtccgcc gcaatcagtt gcaattacag gaatgccgca tgacattgct tccagatata
    15541 ctatgccaaa tgattcatgt ctgctgggaa gaatgaatgt atcactttcc ctgaaaagtt
    15601 cggcaatgtt attcctttcg ggatttctga taagtgtaat tataccattc agtttatttt
    15661 cttttataaa attttcaatg tattgttcca tcggtcccga tcccgcaatg gtaatttcaa
    15721 aattacacga atgctgaacg gctttcataa ttccattcat cagttcttta attcctttct
    15781 gctcgacaaa agaacagact gttaaaaatt taatcttatg atcatctgat ttattttcag
    15841 cttctgacgg agtaaaaata ttctcattta caagatttgg aataataaca ggtgttggaa
    15901 ttttgaatga ttcaattttt tttgacaaag agctgcttac tgcaattacc ctgtcggcat
    15961 tacgaagcgg ttcagaaatt ttagatgata tatttccatt ttctaaaaat ttctcaaaag
    16021 ggaacggacc catgtgctct gtaatgacat aaggaatatt atattttttt ttcaattcca
    16081 ttgcagcaaa acctcccggg aatgaaacat gagcatgaat tagatctatc tctccgaatt
    16141 cagatctcgc tttcagaaaa tttgattcat gtgcttttac aatattattt atttcaccgc
    16201 caagctttgg agtccaggtc agtgctgaat tataaagttc aatcagattt ttccttaaac
    16261 taacttcaat tttcgcagat gacttaaatt ttttcaaagt cttaaagtaa atgtccggcc
    16321 tggaaatact caaataatta ttactgcaaa tggaaacaac aaaatttata tcctcatgaa
    16381 attccgcaag agcttctgcc tgctctctga caaaaattcc gtccagcggg ttttcattgt
    16441 caggatacca agatggaatt atataaatgt tcatagtctc agattaattg ttattattat
    16501 taccatttac attttcggtt gaaaattcaa gatctgcctt ttttgaagct ttgatcataa
    16561 ttgtattata tgtaatgatc tgaataattt caaaaataac atataaagca attccgagaa
    16621 caaaatttcc aagataaaat cccgctacta tagaaagagg gcggtaaata aaagcaaaga
    16681 tattcaggat catagcttct ttttgtttct ttaaaattat cattgcgctg ctggtcggag
    16741 aactgataaa agtaaacata gtcagtatgc atagatattt tgcaatgaca ccggcttcag
    16801 tccatttcgt accgaataca aatctgaaaa tatcctctcc gaaaaaaaat aagatcagta
    16861 caggaataat gcttaacaga aaaagtactt tccatgattt aaaaatgagt tcagtaattt
    16921 tttgtttatt gtaaaaagct tcagagatct tttgataaaa gacctgacca atggatgcgc
    16981 cgatcacaat tccggggatg gataaaattc ttaaagcaaa agaatatgaa cctgtcacgg
    17041 attcagaaaa taaatatgtc agaataaaaa ttaatgaatt aacagaaagc gtatcaaaaa
    17101 aagcgcttgg taaattgaat aatggaaatt ccttatactt taaagaaaca gatcttaact
    17161 caggaatatt agattcattt ttatttgtat tgaaaattct tacatctgat tttttataaa
    17221 gaaaaactgc aggtaacagt tgagagatag cagagctgat gatcagacca aatgaattat
    17281 attttaataa tccgagaatt aatgaggatg tggaatttac tgatgactga gaaacccttg
    17341 aacctgaaat tattttgtat tccttttttc tgttgaacca gtaagtcaat gcctgaactg
    17401 atcctgtcag aaatagaacc ggtgcaatta aaaaaagcca gttttctatt gttgcagttc
    17461 ctagtaattc tgcgatgtat cctttgaaga ttaagacaat taccaatact aaaaatgaga
    17521 gaattgagat cagataaatg caaagtctga gaagtgataa tgcttcagaa tcagattttg
    17581 gaagaacgat cgcaagttca tatcgcagac aacacataac ggtcaggata ctgatgatgg
    17641 aagtgaatac agcaaagagt ccgtaatctt caggagtgta aagtcttgat aaaaccggtg
    17701 atagtaaaaa cgaaatgatt tgaactataa ttgtccctga tgcaagtata gatacatttt
    17761 tggaaaattg cttttgcctt atttcattta ttattcccat atttattaaa aaggataatt
    17821 tataatgaaa taattatttt catgaatttt agctatgatc tttaccggtt taattaagat
    17881 aaaaatagga tcggattttt taatacagaa aaaagtctga atataaattc ttaagtgtta
    17941 tagttatgaa gattagcatt ataaaacatc atctgaattt tatatctgag attatctata
    18001 tctctctgat agttctgttt cttcataccg cttacattcc tgtgctgata ttttctgaga
    18061 gcgagagtgg tagccgtctt tacgtaattc ttttgatcta ctcccacagg acaccagccg
    18121 tatttaaacc gccgcattac atacggaaga tgatgaatgt gatcaggcgg gataaatccc
    18181 agactgacct catcctgcag ctctttaaga attgtcttgc cttcaaaata aactgagatc
    18241 tgaaaaatgg aaaaggtaac aaataaataa agtatgagaa aaagaaatcc gaatattgta
    18301 ctatcctcaa aacttacaga aagattccag ctaaaatgta aaaatactgc aaggaaaaat
    18361 cccatcggta caagtatgaa ttttaagatc atgggtttga attttgcata gccgaggaaa
    18421 gctccgaaag tcgcagtcga gagacaatgc attactgctg aaaataatgt cctgatgata
    18481 acaagcatca gccatgatgc aggtgtagta ccgtaagaca tgaagtaaag gaaattttca
    18541 gacatgccga atccaagtcc gatcgcaccg ccaagtactg caccgtccac tcctccgtca
    18601 aatcttttgc tgaatgaaac aattattaaa aaaattcctt tggtagtttc ctctataagc
    18661 ggtgctgtaa ttactgctcc ggacagattc atcagttcgc tcgggttatc atcagatact
    18721 gctcttatca attcattcag cggaatctga aatatcagac ttccaataat accaagcaag
    18781 atcgcaccgg tcgcacccca gaaaaaattc aacagtacaa accaaaacgg ctctctctca
    18841 tatctgtcaa gataccagat tatgatcaga tagataaaca ttggtactac tgcggctatc
    18901 agtgacgcaa atgccaggaa taatcccatt aaatatttat ttctttttta gtttaattaa
    18961 ataaaagatc tttgtcaaaa actcttgttt agtattatcg aaaattattc aagcatgatc
    19021 aataattcat tcttttccac cgaacacatc tcttccacac agatcttttt tattattgaa
    19081 tctttctttg ctttaatttc attttccatc ttcatcgctt caagaactaa aattacatca
    19141 ccctttttta cttcctgacc ttcggttaca ttcatcttct ttattatacc gggcatcgga
    19201 gataatatat ctgacttaga tttatcatcc gatttatttc ctgcaaactg ctctttcaga
    19261 atatcgaatt cacttttaca tgttactctg tgagtcaaag aatcgatatt tatctcagta
    19321 aaattttcat cagtattttc ttctgctgtg atataataat ttttattgtt gatcctgaga
    19381 atcagtacat ttggattcag ccatttactg tcgaattcta tttcctttcc gttcacgatt
    19441 gcagtgttgt cggaaagtaa agtaagttcc tgatttgaat gacttacacc tgtagaatca
    19501 ggatccgcaa aacttatctg aaattttttc atctgtatga atcctgcctt tttttgatcc
    19561 agttactgaa agctccgtta ctgctgatct taaactttcc atttaccaaa ctcttttttt
    19621 cttttaataa aactgatgcc agacagacag caatgttaat ctcttcatca gagttttcat
    19681 tgccgttaat tcttttttga aattcctttt cgataaactg tgtatgatag ttgccttcaa
    19741 tgtattcctt actttccaaa agactaagta aaaatccgat attggttttt atcccgacta
    19801 tcttgtattc ttttaaagct cttttcattt tgtctattgc gatctctctg ttcgctccgt
    19861 atgagatcag ctttgaaacc atagggtcat aatagattga gatctcgtca tcctgctcaa
    19921 tacccgtatc ttctctcatg ccgcatccga gaaatttttt catgtaagtg atctttcccg
    19981 tcgaaggcat aaagttatta taagaatctt cagcgcatat tctacattcg atcgtatgcc
    20041 ctgtgaattt tatgtcttcc tgtttatatg aaagtttttc tccgtttgcg atctttagct
    20101 gctctctcac aaggtcaagg ccggtgcgga tctccgtgat cggatgctcc acctgaagtc
    20161 gggtatttac ttcgaggaag tagtaattat ttttattatc gactatgaat tctactgttc
    20221 ccgcgtttga atatccggca gcttttacaa ttctctttgc acattcaccc atttcatgac
    20281 ggagattttc gtccattatc actgaaggcg tctcttcgat aattttctga tgacgtctct
    20341 gcattgagca ttccctttca ccgagatgaa taacattgcc atgttcatct gcgagtatct
    20401 gaaattcaat atgtcttgga tctgcaatat acttttcgat aaagacgctg tcatctccga
    20461 atgaagaaag cgcttcactt tgcgccatac gcaatgaact ttctatatcc gcttcttcat
    20521 tcacgacacg cattccttta ccgccgccgc ctgcagatgc ttttatcaga acaggatatc
    20581 cgatctccga tgcgatggat tttattttgc tcatgtcctg caaaggcttg tccgtaccgg
    20641 gtacgactgg aacatcattt tttaatgcca gtccttttgc atctgtctta cttccgagga
    20701 gatcgataga ttcggcagaa ggaccgatga atctaattcc gttgtcttta cacatcttcg
    20761 caaagaatcc tctttctgaa agaaatccgt aacccggatg tatagcttcc gcgcctgtct
    20821 tttttgcaat gtctatgatc ttatcagcta aaaggtaact ctttgaggaa ggtgattcac
    20881 ctaggtgata tgcttcgtct gcaagttttg tgaaaagtga gtatctgtca aaatcggaat
    20941 atactgccac tgtctttatt cccatttcct tagccgtgga aattatcctg acggctatct
    21001 cgcctctgtt ggcgatgagt atctttttaa acaagtttta aaattaattt taaatcccgt
    21061 aaatttacta attgtgtttt gatattaaaa ggaagtaatc ggaagtgcga ctgtaaattt
    21121 taaatgttaa attttaaata acaaatcatt tgtaatagat tgagattcaa ttgttttggt
    21181 gctaaagata taccttagcg tctttgcgtc ttcgaggcaa atcaggcaaa ttctgtctaa
    21241 aaatgaaaac agttgaaagg aatcaaatca gaatttaatt ttaaatctta aattgagata
    21301 tttgcatgta taaaaaatta acccaaaaaa ttaatatcat gcaaagaata gatctcataa
    21361 ataaagtagc tgattcgatc gacagattag acggtaaagc attcagcgaa ttcatcacag
    21421 aagacggttc atttaagtat gcaaataatg aacctgtaaa aggcagaaaa aatattgaag
    21481 aatatgtgag tgcctttttt aaatttgtca aatcaggaaa gcataaggtc atcagaatct
    21541 gggaagacgg cgacagcctc gtctggcagg gtgaggtcac ttataccaga caagacgata
    21601 aggtgatgac ctttcctttt gtgaatgtat ttaaaatgga tggcgatctg atcagcgatt
    21661 atcagattca tattgacaat actccgttat tcggagaata aatacttcta ctatttattt
    21721 attatttaat ctttaaaatt taaatttata tcattaaaac gaattataaa aaagttcaag
    21781 attcattttt ttcaacttac tttttttgaa tgactcccct ctgaaagcat aaggggacaa
    21841 gctccaa
//
LOCUS       DAORVQ010000031        21314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_944_length_21314_cov_4.979397, whole genome shotgun sequence.
ACCESSION   DAORVQ010000031 DAORVQ010000000
VERSION     DAORVQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 21314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21314
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_944_length_21314_cov_4.979397"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1023
                     /locus_tag="PLX80_04370"
     CDS             118..1023
                     /locus_tag="PLX80_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20065.1"
                     /translation="MHKIIYLILLIIITASSPVQSQELYIVSKAAANLSKDRIEFRNN
                     VMSYDNFQYYHNSFEINYGILGNLTLYNNVFYSTETNTDFFGNYHGALRYRFLNFDKP
                     NSHFRTAIQSGVTIPVNAQPIVGDQIEYELHPGHVIKLYNFIEDITVPSVDFHTTDNY
                     TIDNSIIGTYLINKFSATGEMGYNINVPKKDFKFGNYFNWDLSFGYLVLPKVYKSYDD
                     VNLNIYSESKAYYFEKNEFLNSPIINSGGFRLDTYIGVQAIFLSSIMAELSYKIPIHT
                     NEYAEINFGNRTSALLISFRYLFFL"
     gene            1056..3044
                     /locus_tag="PLX80_04375"
     CDS             1056..3044
                     /locus_tag="PLX80_04375"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQY20066.1"
                     /translation="MTREKFYLILLYILITGSLFSQVPVYKDFPVHEIEKQNFLRNSP
                     YINSFYQSDTNINITYYKLDLNITYQPDYLTGIVSIQFATRVNSLKQVFFDLSNNMTI
                     DSIIIQNTVQSFSHVMDKIHITLPSQLNNGSSEIIKIFYKGIPIPTGFGSFIFGSHDN
                     GTSPAIWTLSEPFGASDWFPCKNSPADKADSSDVIVTCSNTLTAVSNGNLILETDNGN
                     GTKTYGWHNSYTIAVYVISLAIADYAQYNSFFRYSLNDSMPIFNYIYPENLDGLKSQL
                     DKTDNMLEFFSDVYGLYPFINEKYGHAEFGRIAGMEHQTISSMGIFNDNIMAHELAHQ
                     WFGDKITCRTWENIWLNEGFATYGEALYNEYIYGQTGYDEFIKYRMADAKRAVGSVYV
                     QDVNSVAQIFSGDRSYAKGCVVLHMLRGVVGDQNFFDILKTYSADTNVAYKTAVTEDF
                     KRVAESVSGMDLNYFFNEWIYGENYPRYIVDWTTEEINSAQYNATFNILQQQNTSPLF
                     FTMPVQILISFQNKDTIVTVLNNSQYQSFTFTFGSKPLSFKIDPDNLILKEVRGENII
                     PVTFSLGQNYPNPFNPSTTIVYQLGRPSNVNFVIYDITGRKILTLLTDQIQREGVYKY
                     EFNFANLSSGVYFYYFTATDTQNNNSYFEETKKMILIK"
     gene            complement(3227..3370)
                     /locus_tag="PLX80_04380"
     CDS             complement(3227..3370)
                     /locus_tag="PLX80_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20067.1"
                     /translation="MKFEIYSFVSPCLGGTILTLIFIIKIPLPSTWIPADAGMTKSGG
                     MTK"
     gene            complement(3393..3524)
                     /locus_tag="PLX80_04385"
     CDS             complement(3393..3524)
                     /locus_tag="PLX80_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20068.1"
                     /translation="MSFLYFKVWWDDIVKRNDIVKRNDKVKGNDNKRVCEVLLTVIL"
     gene            complement(3602..5719)
                     /locus_tag="PLX80_04390"
     CDS             complement(3602..5719)
                     /locus_tag="PLX80_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQY20069.1"
                     /translation="MPEGIKSIMVETEVNETEVKETEAPKEMSASLTKKIKAEKDKIK
                     IIVSDYDEAEYKKMLDLYEKTFNVIKENDLVKGKIVAINGEDVLIDIGSKSDGRVSIN
                     DFSDPDEIAVGKEVEVFLEKIEDKEGQLVLSKKKADSMKMWDKIVSAQEDGSIVKGKC
                     IRRIKGGFVVDISGLSAFLPGSQIDTKPIRDYDEYVGKSMDFKVLKVNNVNENIIVSH
                     KLIIEESMKGQREELLKTIEKGMTMKAVVKAITDFGVFVDLGGLDGLIHITDLSWGRI
                     NHPSDIVDLDQNIEVSVLEFDPEKERVYLGLKQLQKHPWDEIQNKFNIGDKVSGKVVS
                     LTEYGAFIEIEKGIEGLIHVSEMSWTQHITNPTQMVSMGQVMEAQILTIDSENKKLSL
                     GMKQLTPNPWQSLLDKFPIGTKHKGKVCNITNFGIFVELEEGVDGLVHISDLSWTKKI
                     FDIGDLFKIGDEIEVVILGVDVANQRISLGRKQLLENPWDTLEEKYKVGSEAECRIIK
                     PIEKGIIVELPEDLDSFIPASQLSTMPIKNFNELFKAGDSLKAEIIEFDKTNKKIVLS
                     VIEYLKDKEQDEIDGYISKFNLPKKFTSDDIQDKTRSFESEQIDFKIEDIIGEDVPEM
                     NHPPLEKPVKTSVPVADTKSEKPVSEKAPEAKAEEVKTPEVKAPEAKAEEVKSEEKAP
                     EAEVVKSADESAKPEEGAEPKSE"
     gene            complement(5843..6499)
                     /gene="cmk"
                     /locus_tag="PLX80_04395"
     CDS             complement(5843..6499)
                     /gene="cmk"
                     /locus_tag="PLX80_04395"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQY20070.1"
                     /translation="MDKVVVALDGPSGTGKSTTARILAEKLNYLYIDSGAMYRAITFE
                     LLKRGIKPNDTKKIVEITSKADIKFEGEEVFLNGKNITKEIRSLDVTSKVSSVSSIKD
                     VRKILVDKQREYAKANDVVMDGRDIGTVVFPNAKFKFFLVCDLKTRAARRRQDFMEHG
                     MKLPIEKIMAELKKRDSVDSSRKESPLKKSKDAVEVNTTNMIIEEQVNYLFRIVSGLD
                     "
     gene            complement(6879..9485)
                     /locus_tag="PLX80_04400"
     CDS             complement(6879..9485)
                     /locus_tag="PLX80_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HQY20071.1"
                     /translation="MEKTGPMIRTPDQRLRVFISSTLKELADERNSVRSSVESLRLIP
                     VMFELGARPHPPKDLYQAYLLQSNIFIGIYWQSYGWIAEHETISGIEDEFRLSGKLPR
                     LIYVKEPALEREIKLNGLLDSVRSEGDVSYKSFSTSEELQKLVTDDLAILISERFYSA
                     ENQNSEVSDTPKTKTYHSNLPSILPELIGREVDIENLREVIIKEQQKLITVTGPGGIG
                     KTFLTKALAGTLKDHFEDGIFFIDLSDIKDEKSVFIEIAALTGIQISEPENIVRQISD
                     FISSLKILLILDNFEHLSSSTSEITKLSLNCPGLTIIITSRDSLNMSFENEYRVSALG
                     VPAIIDEFNKIISSPSVKLFYKKARSADNNFNLNENNIHDVAEICRILEGIPLAINLA
                     AAKVRIFSPGMILNRLSKKLLILSGGSSDAPARHKTMRAAIEWSIDLLNKDEKILFRR
                     LAVFVNGFDYEALESICTDCSEGPDELAESLISKNIIKKDIEIHGITKYIMPGMILEY
                     AKELFDDSDEKENIKIKHAYYYLNSAKKDIKNFSGENISAASKKWGADAENVIEAAIT
                     FLQNEKYSELVNLIFSLWQLFWIFDYESELEKKIDINEIIRYQDKLSEEDKGKLLWLI
                     GASALGKGDYENAEKYLNQAKDIFESSGNKNGYAWAFHLITSMHAASQQENSNQEIIN
                     SLKKSAMLFRETGDFWGECSVLQNSAALETLIKHYSRALKLYDEYEILARKTKNIAQE
                     GYINTMRGWIYINTRKYEQALNHLRKGLQIFKDGTTPEALSYSLLILSYYYFKTKNEI
                     NAFFIAGAQENIKTKYGITQWQMLSSVTELISDKIRNNNDILLRDAYDRGMKMGVYKA
                     CGKAYEMLLGRE"
     gene            9865..11634
                     /locus_tag="PLX80_04405"
     CDS             9865..11634
                     /locus_tag="PLX80_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQY20072.1"
                     /translation="MNRIIFIVFFLMIFTSKIFSQINVENITITGNDYLTRTEISNMM
                     ITRKNTPLSIDQFMLDLKTIRERYKINGYLYMKFIGEQLKFSDDSAFADITLDISEGE
                     VIDIGKINIKGNKSISTDKILSKFETKEGDNLYDAKLNRDIVALLKIYEKSGLPFARI
                     TVDDISIYKTGLPEKDKIKIDLNVIENSRITIDQVRIKGNETTKEYVITRELNLDENR
                     TVDTESLLAMKEKLDRLNIFEFVADPKVYSIKGKDESGLLIEVTEGNTNTFDGILGYV
                     PPVEGVSDGYLTGLVNVSFRNLFGTARKIEAKYFQEVKETQELEFRYFEPYFFSLPLN
                     LNLGFLQRIQDSTYTKRNLDLKGDFLFSEDFTISLVGGYERVIPSDNPNNTIVIADSR
                     ILSSGIQLNYDNRDNIFIPTKGFNYNTFYSYGSKKIFNAAQLQQYGYQDFYSIQRYYI
                     DIEYYYSVFPRLTTLLDLFGGEVRSEKLEESDYFRIGGLNFIRGYRPDQFLASRLVSE
                     NFELRYSVSRKGFLFTFFDAGYYFRPYDEINNYPEQSGFLYGYGLGIRLETGLGVIGV
                     GYGLGKGDSILDGIINFGLINDF"
     gene            complement(11877..12125)
                     /locus_tag="PLX80_04410"
     CDS             complement(11877..12125)
                     /locus_tag="PLX80_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20073.1"
                     /translation="MLHLKYLQQNNLLPSPACGITAVGYLVGYVSCPDNVNGKKREVR
                     IQKNNILCFIRHLLNFKTLNSKIHLKPVYQEKRIFETT"
     gene            12213..14228
                     /locus_tag="PLX80_04415"
     CDS             12213..14228
                     /locus_tag="PLX80_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20074.1"
                     /translation="MKNFNKILLISFYSLFSTFQLYSTPINQFINSVSPPMNANSVVK
                     SSDIVITFEQTMNGSTMTDANVKVFGYQTGLMNVSLDFNSVANTLSINPVNEFKNGEK
                     ISITLTTGIKTLTNQNISPFLYSFRTKALGGTGTFIKAFEINNIANGCFRSGDFDGDG
                     DIDLLLNDKIFKNNGTGLFLYYSTLNINGISLTADFDNDGDLDIIITSNNLNHFFRNN
                     GFGDFFQEFSFPGGVDNYGDFDGNGFLDLTYFSDINANLIHAIKNTAGSFSNDTVYNI
                     TNLAICNPGINNFKKILIEDMDNNGSPDLIVCNSNSSGNIFNYIYCENFIQLNNSGDG
                     IFNSQFIYSHDINDINYFILDSGDPKLFSITNNGNIDILTQEVFLKNVGNGVYSNEQG
                     IGIFGSSIDADFNGDSNIDMLTYYSFVTPLTLFLNDGAGNFSFSYPGDFSLYNTSYTS
                     SDFDNDGDIDIAIKESGNDKVAILLNGDSPLPVELTSFTSDVNSNSVKLNWSTSAEQN
                     NSGFEIQRTFVKNETPDVWTKAGFIIGAGNSNSSSEYSFEDKNLSSGKYKFRLKQIDF
                     NGNFKYYDLSVEVLIGIPASTELMQNYPNPFNPVTNISYRLSENGNVSISVYDNSGRE
                     VKTLVNEFKEAGYYSAEFIGSDLASGIYFYKLTAGNFVMTKKLSLVK"
     gene            complement(14450..15847)
                     /locus_tag="PLX80_04420"
     CDS             complement(14450..15847)
                     /locus_tag="PLX80_04420"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HQY20075.1"
                     /translation="MKKLNLNENFICRIWEEKSNYSDLKSNDGRDVEILEFGNRNIDA
                     GPDYTDAKIKLGGNILSGAIEIHRTTNDWKLHKHQGDGKYNDVILQVALYDDLFSKVK
                     NPIVDQSREIPTVVLSEFLSRSIHCIWKDIINKPSESFRLPCYPKSKEIIYEEKISFI
                     KELGLIRLKHKSEKLGQRKKELNKYIQPHISDKHVLFEYLCEALGYSKNKEQFISLSR
                     KIDIEKINKLKFDKQQTDALIFGLSGFLSDLKIEDEYSEDLFSNWISIRGEIKKEEMI
                     RSEWNFFRLRPPNFPTLRLSYASGLLYEILYNRFTERLIIEFEESTDILKSMNELFSM
                     VNISPYWKCHYNFGKKSKSENKGIGSDRIKDIISNVVFPFMYLIAEDTESENLKNKVE
                     YFFKNNKQKSPSNEISKVMQDQLAVNAINPATEQGLIFLHKFFCIKGKCEECKIGRSV
                     FEEDSVHEPLRIIIY"
     gene            15946..16587
                     /locus_tag="PLX80_04425"
     CDS             15946..16587
                     /locus_tag="PLX80_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY20076.1"
                     /translation="MLILSVMILSGCSGNKKTEEQYLADAKLKLEEKKYDEAIADYRE
                     FLKNYPKSDKAIFAYNEIAGIQISNLSNPQEGIKTYQELAKNFPDTKEAKQSLFMVAF
                     IYDETLKDKTNAINSYQAFLDKYPEDKDPNDKMSESAKTMLEVLKSGKSIEEIIEESI
                     SKSGTSTDTTGTSGSDDSNIKLKKLETDSKESGKKVQKDVKADDGSVEDPAKK"
     gene            16609..17598
                     /locus_tag="PLX80_04430"
     CDS             16609..17598
                     /locus_tag="PLX80_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQY20077.1"
                     /translation="MTETTDYKKYLDPSVISRLSNMELKARMVVEGFIAGLHKSPYHG
                     FSVEFAEHRQYMPGDDLKFIDWKILGKTERYYVKRFEEETNLKTYLLLDISRSMDFKS
                     EDTSSLNNLRKGFFKKYFSSENKEELQTSSDLTKLEYASHLAASLAYLMLLQRDAISL
                     TTYDTHIRSYIPPHSTKANLRLILKELTNIKSLNQTGTAKCLNEIADKVKKRGLVILF
                     SDLFDEQKDVLNALKHFRYNKNEVIIFQIMDPMEINFLEGSPVTLKDMETNEEMYSQP
                     AAMQKIYRESIQEFIQNYKTECIKNNIEFITLSTATSFETALLGYLNKRKRLI"
     gene            complement(17621..18952)
                     /locus_tag="PLX80_04435"
     CDS             complement(17621..18952)
                     /locus_tag="PLX80_04435"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQY20078.1"
                     /translation="MKHSKQIKLEISSDLKKLMCLILISFISVLAFKDSYSQLSDKNI
                     EIDSRDFFYFDPLVFYSKDEGKARLDVYLEIPLENLQFKKNYATKNYDASITYNIKIT
                     NSFNEIVSNENITDLVSTSKAEQKNLESSSKYIVKEFYLNPGNYSLEISITDINTKKE
                     KTEKGSVEITDFVQKDISASNIMLVSNVKEENGKKVITPLIARNIGNLKSIYLFFEVY
                     NSSDNEVVNDFTYTITDSKENILEKDNLVYTLSPGINKYFEKISTSNLIFGEYIIELK
                     NNSTGEIVAYKEFENKLSGMPTNVKDLNILIDQMIYIASSEEITEIKNSKTPELKEKN
                     FVEFWRGKDPSPYTTKNELMVEYYKRVGEANERYSHYIDGWKTDMGMVFIIFGEPNNI
                     ERHPFNEGVKPYEIWEYYNENQQYIFVDETGFGDYKLTIPIWNTDANRIRN"
     gene            complement(18991..20292)
                     /gene="rimO"
                     /locus_tag="PLX80_04440"
     CDS             complement(18991..20292)
                     /gene="rimO"
                     /locus_tag="PLX80_04440"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQY20079.1"
                     /translation="MKVKKDKVKLITLGCSKNLVDSEFIMSQLKSNDIELVEDEDDAE
                     NVIINTCGFIENAKKESIDTIMRAVDRKNKGLLKNVYVAGCLSDRYKDELEKDIPEVD
                     KYFGATDKPHTIIGILKELGGDYKSELLGERSLTTPSHFAYLKISEGCDNPCSFCAIP
                     IMRGVHRSKPLDQIIMEAQILASKGVKEIIVIGQDTTYWGFDINRKRELSQVLRELSN
                     INGIEWIRLMYAYPSRFPEDIIEVIRDTPNICNYIDIPVQHITDNVLKSMRRGITKNT
                     LTGLLEKLRNEIPGIAVRTTFIVGYPDETEKDFIELTEYIKEYEFDRLGVFTYSHEDG
                     TTAAEIPDRIPLKEKLSRQKHLLDIQKEISLKRNMNSNNEVLKVLVDRKENGYYIGRS
                     YRDAPEIDQEIYISNQDLKTGEFYNVRIFDSEEFDLFGEIV"
     gene            20869..21075
                     /locus_tag="PLX80_04445"
     CDS             20869..21075
                     /locus_tag="PLX80_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20080.1"
                     /translation="MKITSAKCPSCGGNLEIPDNKDYITCLYCENTVRVRDAVPVLEI
                     DKVENFAVVGVFKKFAEIHLKFTI"
BASE COUNT         6838 a         3789 c         3207 g         7480 t
ORIGIN      
        1 attcgaatga gcccgaaacg aaattttaat tttcattaaa aacccctttt tatctgaact
       61 tatttatgaa ttactttatt tcacaatctt aattacattt tacaaaataa tttcacattg
      121 cacaaaatca tatatttaat actgctaatc attattactg cttccagtcc ggtacaatca
      181 caggaattat atatagtatc aaaagctgct gccaatcttt ccaaagacag aattgaattt
      241 agaaacaatg tcatgagcta tgataatttt cagtattatc ataattcatt tgagatcaac
      301 tatggcatac ttggaaacct gacattgtac aataatgtgt tttattcaac tgagaccaat
      361 actgattttt ttgggaatta tcacggtgcg ctcagatacc gatttttaaa ttttgataaa
      421 ccaaattcac acttccggac agctattcag tccggtgtaa caataccagt caatgctcag
      481 cccatcgtag gagatcagat agaatacgaa ttacacccgg gtcatgtaat taagctttat
      541 aatttcatag aagacattac agtgccttca gtagattttc atacaaccga taattacact
      601 attgataaca gtatcatagg cacatactta ataaataaat tttcagcgac aggtgaaatg
      661 ggttataata ttaatgttcc gaaaaaagat tttaagttcg gaaattactt taactgggat
      721 ctttcatttg gttaccttgt cctacctaaa gtttataaaa gttatgatga tgtaaattta
      781 aatatctatt ctgaatccaa agcttattat tttgaaaaaa atgaattcct gaattctccc
      841 atcataaact ccggaggatt cagacttgat acatacattg gagttcaggc aatatttctt
      901 tcttccatta tggcagaact ttcatataaa atacctatac atacaaatga atatgctgaa
      961 ataaattttg gtaaccgtac atcagccctg cttataagct ttaggtatct tttctttctc
     1021 tgataaaaat cactaattaa ttttgtaaat ccggaatgac acgggaaaaa ttttatttga
     1081 ttttgcttta tattctgatc accggatctt tattttcaca agtcccagtc tataaagatt
     1141 ttccagtaca tgaaattgaa aaacagaatt ttctcagaaa ttcaccttat ataaattcct
     1201 tttatcagag cgatacaaat ataaatatta cctattataa acttgatctg aatattactt
     1261 atcagcctga ttatcttacc ggcattgttt caattcaatt tgcaaccaga gtcaattcat
     1321 taaagcaagt tttctttgat ttaagtaata acatgacaat agattctata atcattcaaa
     1381 atactgttca atcattcagc cacgtaatgg ataaaataca tatcacgctt ccttcgcaat
     1441 tgaataacgg ttcttcagaa ataattaaaa tcttttacaa aggaattcca attcctaccg
     1501 gtttcggaag ttttattttc ggatctcatg ataatggaac gtctcccgcg atctggacac
     1561 tgagcgaacc cttcggtgca agtgactggt ttccctgtaa gaattcccct gctgataaag
     1621 ccgactcttc cgatgtcata gtgacatgca gtaatacact taccgctgta tcaaatggaa
     1681 atctcatatt ggaaacagac aatggtaacg gcacaaaaac ttatggctgg cataattctt
     1741 atactattgc agtctatgtc atttcactcg ctatcgcaga ttatgcacag tataacagct
     1801 tcttcagata ttcactaaat gactctatgc cgattttcaa ttatatctat cctgaaaatc
     1861 tggacggatt aaaaagtcag cttgataaaa ccgataatat gcttgaattt ttttcggatg
     1921 tgtatggact ttatcctttt ataaatgaaa aatacggtca tgcagaattt ggtaggatag
     1981 cgggaatgga acatcagaca atctcatcca tgggaatttt taatgacaat ataatggcgc
     2041 atgaacttgc gcatcaatgg tttggcgata aaattacctg ccgcacatgg gaaaacatct
     2101 ggctcaatga aggttttgca acttatggtg aagccttgta taatgaatac atctatggac
     2161 agacgggcta tgatgaattt attaaataca gaatggctga tgcaaaaaga gcagtcggct
     2221 ctgtttatgt acaggatgta aattctgttg cacagatatt cagcggggac aggagttatg
     2281 caaaaggctg tgtggtactt catatgctca gaggagtcgt tggtgatcag aatttttttg
     2341 atattctgaa aacttattct gctgatacaa acgttgctta caaaactgca gtaactgaag
     2401 attttaaaag agtagctgaa tcagtctcag gaatggatct taattatttt tttaatgaat
     2461 ggatctacgg tgaaaattat ccaagatata ttgtggactg gactacagaa gaaattaatt
     2521 ctgcacagta taatgcaacc tttaacattc ttcagcagca aaatacatcg ccactctttt
     2581 tcacaatgcc cgtgcagatc ctgatcagtt ttcaaaataa agacacgata gtcacagtgt
     2641 taaataactc gcagtatcag tcatttacat ttactttcgg cagtaaacct ctgagcttta
     2701 aaattgaccc tgataattta atactgaaag aagtcagagg cgaaaacata atccctgtta
     2761 ctttttctct cggacaaaat tatccgaacc cattcaatcc ttctacaaca atagtatatc
     2821 agctaggcag accatcaaat gtaaattttg tgatttacga tatcacaggc agaaaaatat
     2881 taactttgct tactgatcag attcaaaggg aaggtgttta caaatatgaa ttcaattttg
     2941 caaatctttc ctccggagta tacttctatt acttcactgc gaccgatact caaaacaata
     3001 attcctactt cgaagaaact aaaaaaatga tcttaattaa atgaaccccc tctctacagc
     3061 gatgtccggt ttataccaca aagacacgga gacacaaagg aattaatttc aaatttcaaa
     3121 tttcaaattt caaatttcaa atttcaaata ttatattata agataactgt taataatact
     3181 tcacaaactc tcttattctc attacgcttt actttgtcat tcctctttac tttgtcatcc
     3241 caccagactt tgtcattcct gcatccgcag gaatccatgt cgaaggaagc gggattttga
     3301 taataaatat taatgtaaga atagtaccac caagacacgg agacacaaaa gaataaattt
     3361 caaatttcaa atttcaaatt tcaaatatta aattataaga taactgttaa taatacttca
     3421 caaactctct tattgtcatt accctttact ttgtcattcc tctttactat gtcattcctc
     3481 tttactatgt catcccacca gactttaaaa taaaggaatg acaataacat cagtgttttt
     3541 tacaaaaaaa acccggataa ttacttaccc gggttatata aaataaatta tttcttatta
     3601 tttattcaga ttttggttct gcgccttctt caggttttgc agattcatct gctgatttta
     3661 ctacttcagc ttctggggct ttttcttctg attttacttc ttcagctttc gcttcaggag
     3721 cttttacttc aggagttttt acttcttcag ctttcgcttc aggagctttt tcggaaacag
     3781 gtttttctga tttagtatca gcaaccggaa cagatgtctt aacaggtttt tccaacggag
     3841 gatgattcat ttccggtacg tcttcaccta ttatgtcttc gatcttaaag tcgatctgtt
     3901 cggattcaaa acttctggtc ttatcctgaa tatcgtcaga tgtgaatttt ttaggcagat
     3961 tgaatttgct gatataaccg tcaatctcat cctgctcttt atctttcaga tattcaatta
     4021 cagataatac tattttttta tttgtcttat caaattcaat gatctcagct ttaagtgaat
     4081 cacctgcttt gaaaagttca ttgaaatttt taattggcat tgtcgaaagc tgtgacgcag
     4141 gaataaatga atccagatct tcaggaagct ctacgataat tcctttttcg attggtttaa
     4201 taattctgca ttcagcttca gaaccaactt tatatttttc ctcaagagta tcccaaggat
     4261 tttcaagtaa ttgctttctt ccaagagaga tcctctgatt agcaacatcc actccaagta
     4321 taacgacttc aatttcatca cctatcttga acagatcacc aatatcaaat atcttctttg
     4381 tccatgaaag atcagaaata tgaacaagtc cgtccactcc ttcttcaagc tctacaaaga
     4441 ttccaaagtt tgtaatgttg catacttttc ctttatgctt tgtaccgatc gggaatttgt
     4501 caagaagact ctgccatgga ttaggtgtga gctgtttcat tccaagagaa agtttcttat
     4561 tctcggaatc aattgtaaga atctgagctt ccattacctg acccattgaa accatttgtg
     4621 taggatttgt aatatgctgt gtccatgaca tttcactgac atgaatcaga ccttctatac
     4681 ctttttcaat ttcaataaat gctccatact cggtaagtga tactacttta cctgaaactt
     4741 tatcaccgat attgaattta ttctgaatct catcccaagg atgcttctgt aactgtttca
     4801 atccaagata tactctttcc ttttccggat cgaattcaag aactgatact tctatattct
     4861 gatcaagatc tacaatatcg ctcggatgat ttattctacc ccatgaaaga tcagtgatat
     4921 gaattaatcc gtcaagaccg ccaagatcaa cgaatactcc gaaatcggta atagctttta
     4981 caacagcttt cattgtcata cctttttcaa tagtcttcag aagttcttct ctctgtcctt
     5041 tcattgactc ttcaatgatc agcttatgtg atacaattat attttcgttt acattattta
     5101 ctttcagtac tttaaagtcc attgacttgc cgacatactc atcataatct ctgattggtt
     5161 tagtatcgat ctgtgaaccg ggcaggaatg cagaaagtcc tgaaatgtca accacaaatc
     5221 cacctttgat ccttctgata catttacctt ttactatgga accatcttcc tgtgcagaga
     5281 caattttgtc ccacattttc atagaatcag ctttcttttt tgaaagcacc agttgtcctt
     5341 ctttatcttc tatcttctca agaaaaactt ctacttcttt tcctactgcg atctcatcag
     5401 gatccgaaaa atcatttatc gaaactctgc catcagattt agatccaatg tctattaata
     5461 catcttctcc attgatagca actattttac cttttacaag atcattttcc tttattacat
     5521 taaaagtctt ttcatacaga tcaagcattt tcttgtactc agcttcatca taatcactta
     5581 cgataatttt gatcttatct ttttcagctt tgatcttttt tgtcagagat gcagacattt
     5641 cctttggtgc ttcagtttct ttcacttctg tttcatttac ttctgtttct accattatag
     5701 attttattcc ttccggcata gtttgctttt gtatgccatt ttaactttta ctgtttttaa
     5761 ttattacagt atcagtttgt ttaattaatt aattccccta agggctttat tataaatatt
     5821 tttaattttc tgtataaaaa ctttaatcca gaccacttac tattctaaac agataattta
     5881 cctgctcttc aattatcata tttgtagtat tcacctctac agcgtccttg gattttttca
     5941 aaggactttc ttttcttgag ctgtcaacag aatctctttt cttaagctct gccataattt
     6001 tttctattgg cagtttcatt ccatgttcca taaaatcctg tctccttctt gccgctctcg
     6061 tcttaaggtc gcagacaaga aaaaatttaa attttgcatt aggaaaaact actgttccaa
     6121 tatcccttcc gtccattacc acatcatttg cctttgcata ttcacgctgc ttatccacta
     6181 gaattttcct gacatctttt attgaactta cactgctgac cttgcttgtt acatcaagcg
     6241 atcttatctc tttggtaata tttttaccgt taagaaaaac ttcttcgcct tcaaatttta
     6301 tgtcagcttt acttgtaatt tcaactatct tttttgtatc attcggttta atacctctct
     6361 taagtaattc aaatgtaatt gccctgtaca ttgctccgct gtctatatac aaataattca
     6421 gtttctctgc cagtattctt gccgttgtac tcttacccgt tcccgaaggt ccatctaacg
     6481 ctactacaac tttatccatt actaaacaat tgacgttatc aaattataaa taatttttta
     6541 aattaaagaa ataattcaga caattcaaat tattgaattt cttagttgca ttgaaatgcc
     6601 cgtttaaata aagataataa cgaataacct tgaggtaaag aaatgcggtt tgttcgaaat
     6661 tgcttttaat tattccatac gttcagttat aaattcaact ttactctgaa cgacacataa
     6721 agtcgctaaa ttaatacata ttttcaatgt atttttaata gactgaagag aaaattcttt
     6781 cagcttacaa tattatgaat ttctttgagg aatgaataat ttctgaatgc ttttttctct
     6841 tatataatct gaaaataaat tcaatttatc aaaacaaatt attcccggcc taacagcatt
     6901 tcataagcct ttccgcatgc tttatatact cccatcttca tacctctgtc ataagcatcc
     6961 cttagtaaaa tatcattatt attcctgatc ttatcgctaa tcaattctgt tacggaagaa
     7021 agcatctgcc actgtgtgat gccgtatttt gttttgatat tttcctgtgc gcctgcaata
     7081 aaaaatgcat taatttcatt ttttgttttg aaataataat aggataatat gagaagggaa
     7141 tagcttagag cttcgggtgt tgtaccgtct ttaaaaattt gcaacccttt ccttaaatga
     7201 tttaaagctt gttcgtattt tctggtattg atgtatatcc aacctctcat agtatttata
     7261 taaccttcct gtgcaatatt ttttgtcttc cttgcaagta tttcatattc atcatataat
     7321 ttcaaggctc tcgaataatg ttttataaga gtttcaagtg cagctgagtt ctgcaataca
     7381 ctgcattcac cccagaaatc accggtctct ctgaaaagca ttgccgactt ctttaaagaa
     7441 tttattattt cctgatttga attttcctgc tgacttgcag catgcattga tgtaatcagg
     7501 tgaaatgccc aggcatagcc gtttttatta cctgaagatt caaaaatatc ctttgcctga
     7561 ttcagatatt tttcagcatt ttcataatca ccttttccaa gtgcgctggc accgatcagc
     7621 cacaagagct tacctttatc ttcttcagat agtttatcct gatacctgat tatctcattt
     7681 atatcgatct ttttttcaag ttctgattcg taatcaaata tccagaaaag ctgccataat
     7741 gaaaaaataa gattcactag ttccgaatat ttttcattct gtaaaaaagt aattgcagct
     7801 tctatgacat tctcagcatc agctccccac ttttttgaag cagcggaaat attctctccc
     7861 gagaaatttt ttatatcctt ctttgcagaa ttcagataat aatatgcatg cttaatttta
     7921 atattttcct tttcatcaga atcatcaaat aattcctttg catattcaag gatcattccc
     7981 ggcattatgt atttggtaat accgtgtatt tcaatgtcct tttttattat gttttttgag
     8041 atcagtgatt cagctaattc atccggacct tcactgcagt cagtacatat actttcgagt
     8101 gcttcataat caaatccgtt tacaaacaca gcaagtcttc tgaataaaat tttctcgtct
     8161 ttattaagaa gatctatact ccattcaatt gctgctctca ttgttttatg acgggcagga
     8221 gcgtcagaac ttcccccgga aagtatcagc aattttttag aaagtctgtt caatatcatt
     8281 ccgggtgaaa aaattcttac ctttgctgca gcaagattta tcgcaagcgg aataccttca
     8341 agtatacggc agatctctgc aacatcatga atattatttt cattaagatt aaaattgtta
     8401 tcagctgatc tggctttttt ataaaaaagt tttacagaag gagacgaaat gattttatta
     8461 aattcatcaa ttattgcagg cactccaagt gcagaaactc tgtattcatt ttcaaaagac
     8521 atatttaaag aatctctgct cgttataatt attgtaagtc cgggacaatt taaagataat
     8581 ttggtaatct cagaggttga tgaagaaaga tgttcaaagt tatccagaat caataaaatt
     8641 ttaagtgatg aaataaaatc agatatctgc ctgacaatat tttcgggttc agagatctgt
     8701 attcctgtca atgcagcaat ttcaataaaa acactctttt catctttaat atcggaaaga
     8761 tctataaaaa aaatcccgtc ttcaaaatga tctttcaatg tccctgctaa tgcttttgta
     8821 agaaatgttt tacctatacc tcccggtcct gtgacagtaa tcagcttttg ctgctcttta
     8881 ataataacct cacgcagatt ttcaatatcc acctctcttc caatcagctc cggtaatatt
     8941 gaaggcaaat tagaatgata ggtttttgtt ttaggggtat ctgaaacttc agagttttga
     9001 ttttctgcag aataaaatct ttcgctgatc aatattgcaa gatcatctgt tacaagcttt
     9061 tgtaattctt ctgatgtaga aaaagattta tatgaaacat ctccttccga ccttacagaa
     9121 tcaagcagac cgtttagctt tatttccctt tccaatgcag gttctttaac ataaattagt
     9181 cggggaagtt ttccggataa acggaattca tcctctatgc ctgaaatagt ttcatgctca
     9241 gctatccatc cgtaactttg ccagtaaatt cctataaaaa tattgctttg taataaatat
     9301 gcctggtaca gatcttttgg aggatgtggt ctggctccga gctcaaacat aacaggaatc
     9361 agccgaagac tttccacaga tgatctcacg gaattccgtt catctgcaag ctcttttaat
     9421 gtagagctga taaagactct gagtctctgg tcgggagttc tgatcattgg tcctgttttt
     9481 tccaaattgt ttttaaagaa attgttttaa agaaattgtt ttcggagaaa attagtatat
     9541 aatataaaaa aattgatttt ttactgaaat gaaaaattta aagtggaagt aatttattct
     9601 aacaatttta aataataaaa tttctaatac tcaaaataaa atttccgtta agactctgca
     9661 tctttttgga ataatgtttg ggtgtgaaat acaattgctt tacactgttt tagtttcaat
     9721 ttataattca cgaagattat tctttaaaag aatcagatat atcataagtt tctattgagt
     9781 tctgataaat taaattaaaa aaattcatca atgaattttt aaaaatacat tttatattta
     9841 attcagttga attagtttta tcctatgaac cgcattattt ttatagtttt tttcttaatg
     9901 atatttacct caaagatctt ttcgcagatc aatgttgaga acattaccat tacaggaaat
     9961 gattatctca ctcggactga aatttctaat atgatgataa caagaaaaaa tactccgttg
    10021 agcattgatc agtttatgct ggatctaaag actattagag aaagatataa aataaatggt
    10081 tatctgtata tgaaatttat cggtgagcag ttgaagttct ctgatgattc agcttttgca
    10141 gatattactt tagatatttc tgaaggtgaa gttattgaca taggtaaaat taatattaaa
    10201 ggaaataaat ccatttcaac tgataagatc ctttcgaaat ttgaaactaa agaaggtgat
    10261 aatctttatg atgccaaact taaccgtgat attgttgctc ttcttaaaat ttatgaaaaa
    10321 tcaggattac catttgcaag gatcactgtt gacgatattt caatttataa aaccggtctg
    10381 cctgaaaaag ataaaataaa aatcgacctt aatgtaattg aaaacagcag gatcacgatc
    10441 gatcaggtta gaattaaagg aaatgaaact acaaaagaat atgtgatcac ccgtgaacta
    10501 aatcttgatg aaaacaggac agttgatact gaatcacttt tagccatgaa ggaaaaactc
    10561 gatcgtttaa atatatttga atttgtcgct gacccgaaag tttattccat taaaggcaaa
    10621 gatgaatcag gtctgttaat tgaagtgaca gaaggaaata caaatacgtt tgacggaatt
    10681 cttggatacg taccacctgt cgagggagtg tcagatgggt atcttacagg tcttgtcaat
    10741 gtttctttca gaaatctatt cggtacagcg agaaaaattg aggctaaata ttttcaggaa
    10801 gtaaaagaaa cccaggaact tgaattcaga tatttcgagc catatttttt ttctcttccc
    10861 ttgaatctga atctcggatt cctgcaaaga attcaggaca gcacttacac taaaagaaat
    10921 cttgatctaa aaggtgactt ccttttttca gaagacttta ccatatctct ggtaggcgga
    10981 tatgaaagag ttataccatc cgacaatcct aataatacta ttgtaattgc tgactccaga
    11041 atattatctt caggtattca gttaaattat gataacaggg ataatatttt tattccgaca
    11101 aagggattta attataatac tttttattcc tacggaagta aaaaaatatt taatgcagct
    11161 cagcttcagc aatatggcta tcaggatttt tattctattc agagatacta tattgatatt
    11221 gaatattact actctgtatt cccccggctt acaacccttt tggatttatt cggaggtgaa
    11281 gtcagatctg aaaagctgga agagtctgat tatttcagga tcggaggttt aaatttcatt
    11341 cgcggctaca ggcctgatca gtttcttgct tcacgtcttg tttctgaaaa tttcgaactt
    11401 cgatattccg tttccagaaa aggttttctc tttacattct ttgacgccgg atattatttc
    11461 agaccatatg atgagataaa taattatccc gagcaaagcg gattcctgta tggttatggt
    11521 ctgggaatca gacttgaaac cggtctcggt gtcattggag tgggctatgg actcggtaaa
    11581 ggcgacagca tacttgacgg aataattaat tttggtttga taaatgattt ttaagttctt
    11641 tcaatcctgt ttccgctgag atttactaac aattgcggaa tttcaaatca taaattacga
    11701 atgtcaaata ctccggcaac ggataaagct gcggacttcg ccggtaaaga aatttttcat
    11761 cagatataaa aataagcatt gacattcaaa aaaaaaaaaa caattttctc taaaacaaat
    11821 attccattcc tcttcccgaa tgaatctgat ttaaataaaa aattaaagaa ctgtgattac
    11881 gtcgtttcaa atatacgctt ttcctgatat acaggcttca aatgaatttt agaatttaaa
    11941 gttttaaaat ttaataaatg ccttatgaaa cacaatatat tattcttttg tattcttact
    12001 tctctttttt taccattcac gttgtccggg caacttacat acccgaccag atatccgact
    12061 gctgtaatac cgcatgcagg tgacggaagc agattattct gctgtagata tttcagatgc
    12121 agcaattgtc tacaataatt caaataattc ggtaatgacg attcatcctt aaatactttt
    12181 ttttaaaatt tattaactta agtacaaaaa ctatgaaaaa ctttaataaa atattattaa
    12241 tttcatttta tagcttattc tcgacctttc aattatattc gactccgatt aaccagttta
    12301 taaactctgt atcacctccg atgaatgcta acagcgtagt aaaatcatcc gacatagtta
    12361 tcacatttga acagacaatg aacggatcaa ccatgacaga tgcaaatgta aaagtattcg
    12421 gttatcagac aggactgatg aatgtatctt tggatttcaa ttcagttgca aataccctct
    12481 caataaatcc tgtgaatgaa ttcaagaatg gtgagaagat cagcataact ttgactacag
    12541 gtataaaaac tcttacaaat caaaacatct caccctttct atacagcttc agaacaaaag
    12601 cattgggtgg gacaggtact tttataaaag cctttgaaat taataatatt gcaaatggat
    12661 gttttagatc cggagatttt gacggtgatg gagatattga tcttttatta aatgataaaa
    12721 tatttaaaaa taacgggact ggtttatttt tatattattc aactttaaac attaatggca
    12781 tttctttaac cgccgatttt gacaatgacg gtgatttgga tataataatt acttcaaata
    12841 atttaaatca tttttttaga aataacggat ttggagattt ttttcaggaa ttctcatttc
    12901 ccggaggagt agataattat ggagattttg atgggaatgg ctttttagat ttaacatact
    12961 tttcagatat taatgcaaac ttaattcacg ctataaaaaa tactgcggga agtttctcga
    13021 atgatactgt atataacata acgaatcttg ctatttgtaa tccaggaata aacaatttca
    13081 agaaaatatt aattgaagat atggataata atggaagtcc agatttaatt gtttgcaatt
    13141 ctaattcaag tggtaatatt tttaactaca tttattgtga aaattttata caattaaaca
    13201 attcgggaga cggaatattt aactctcaat ttatttacag ccatgatata aatgacataa
    13261 attattttat tttggacagc ggtgatccta aacttttttc aattactaat aatggtaata
    13321 ttgatatcct aacacaggaa gtttttttaa aaaatgttgg gaatggtgtt tattcaaatg
    13381 agcaaggcat tggaatattc ggttccagta ttgatgcaga ttttaatgga gattctaaca
    13441 tagatatgtt aacatattat agtttcgtaa ctccattaac tttgttttta aatgatggag
    13501 caggtaattt ttcatttagc tatccgggtg atttttcatt gtataataca tcttatacct
    13561 caagtgattt tgataacgac ggcgatatag atatagcaat taaagaaagc ggtaatgaca
    13621 aagtcgcaat ccttctcaac ggcgactcac cgttacctgt cgaacttact tcatttactt
    13681 cagatgtaaa ttcaaactcc gtaaaattaa actggtccac ttcagccgaa cagaacaact
    13741 ccggatttga aattcaaaga acttttgtta aaaatgaaac tcccgatgta tggacaaaag
    13801 ccggttttat aatcggcgcc gggaattcga attcatcttc agagtattct ttcgaagaca
    13861 aaaatctttc atcaggaaaa tataaattcc gtctgaaaca gattgacttc aacggtaatt
    13921 tcaaatatta tgatctttca gttgaagtcc tcattggcat tccggcttcg acggaactca
    13981 tgcagaatta tccaaacccg ttcaatcccg ttacaaatat ttcatacaga ctttcagaaa
    14041 acgggaatgt gtcaataagc gtgtatgaca attcgggaag agaagtaaaa acacttgtaa
    14101 atgaattcaa agaggcaggt tattattcag ctgaattcat cggcagtgat cttgcaagcg
    14161 gaatttattt ttataaactg accgcgggga attttgtcat gacaaaaaaa ttgtcgctgg
    14221 tgaagtaaat tcaaattata aattacgaat gtcaaataat aaattatttc ccgccgtagt
    14281 tgatcatggt gagcacagcg ggcgtgatca ataactgcac ttaaatctgt gttcataaaa
    14341 actcccgata attgtttcgc ttcggatgac tcggaggtac ctcccgcttg tacgggaatg
    14401 acatcaaaac ggggatgtca tgaaaaaagt ggattaaata attataacct caataaatta
    14461 ttatcctgag cggctcatgt acgctatcct cttcaaatac tgatcttcct attttacact
    14521 cttcacattt tccctttata caaaaaaatt tatgcaaaaa aatcaaaccc tgttcagtag
    14581 ccggattaat tgcattcact gcaagctgat cctgcattac tttagaaatc tcattcgaag
    14641 gtgatttctg tttattgttt ttgaaaaaat attccacctt gtttttcaaa ttttcacttt
    14701 ctgtatcttc agcaattaaa tacataaatg gaaagacaac attagatata atatctttta
    14761 tcctgtcaga tccgattcct ttattctctg atttagattt cttcccgaaa ttgtagtgac
    14821 atttccagta aggagaaata tttaccattg aaaacagttc attcatactt tttaaaatgt
    14881 cagtgctttc ttcaaattca atgatcagtc tttcagtaaa tctgttatat aaaatttcgt
    14941 agagtaatcc tgatgcataa gaaagtctaa gagttggaaa attcggtggt cttagacgaa
    15001 agaaattcca ctcagatcta atcatctctt ccttttttat ctcacccctt atacttatcc
    15061 agttactgaa cagatcttca gaatattcat cttcaatttt tagatctgaa agaaatcccg
    15121 acagaccaaa tatcaaagca tctgtctgct gtttgtcaaa tttcagtttg ttgatctttt
    15181 caatatcgat cttcctcgaa agagaaatga actgttcttt gtttttggaa tatccaagag
    15241 cttcacataa atattcaaac aagacatgct tatcagaaat atgaggttgt atatatttat
    15301 ttaattcttt ttttctttgc cctaattttt cggatttatg ttttagtctg atcagaccaa
    15361 gttcttttat gaaagaaatt ttctcttcat aaataatttc tttgcttttt ggatagcagg
    15421 gaagtctgaa tgattctgat ggtttattta taatatcttt ccagatacag tgaattgatc
    15481 tgctgagaaa ttcagaaagt acaacagttg gtatttcacg agactgatca actatggggt
    15541 ttttgacttt tgaaaataga tcgtcatata gcgcaacctg tagaataaca tcattgtatt
    15601 taccgtctcc ctgatgtttg tgcaatttcc agtcatttgt ggttctatgt atttcaatag
    15661 cccctgaaag aatatttccg cccagtttta tttttgcatc tgtataatca ggtcctgcat
    15721 ctatgtttct gttaccgaat tcaagtattt ctacatccct gccgtcattt gatttcagat
    15781 ctgaataatt ggatttctcc tcccagatcc tgcaaatgaa attttcattt aagtttagtt
    15841 ttttcattaa tttttaaatt ggttttaact acattaataa aattttttta aaaaaattaa
    15901 ttattactaa taaaacacat gcgaaattta aattttaaat ttataatgct gatcttatcc
    15961 gtcatgatct taagcggatg ttcaggcaac aaaaaaactg aagaacaata tcttgcggat
    16021 gctaagttaa aacttgagga aaaaaagtat gatgaagcta ttgctgatta tagagaattt
    16081 ttaaagaact atcctaaatc cgataaggcg atatttgcat acaatgagat cgccgggatc
    16141 cagatcagca atttaagtaa tcctcaggaa ggaataaaga cctatcagga gcttgcaaaa
    16201 aatttccctg atactaaaga agctaaacag tccttattca tggttgcttt tatatatgac
    16261 gagacattga aggataaaac taacgcaata aattcttatc aggcgttcct tgataaatat
    16321 cctgaagata aagatccgaa tgataagatg agtgaatctg caaagactat gcttgaagtt
    16381 ctgaaatccg gaaaatccat agaagagata attgaagaaa gtatcagcaa aagtggtaca
    16441 agtacagata ctacaggtac ttccggaagt gatgattcga acatcaaatt aaaaaaattg
    16501 gaaaccgata gtaaagaatc aggaaaaaaa gttcagaagg atgttaaagc agatgatgga
    16561 tccgttgaag accctgcaaa gaaatagtaa acttctcaat tataaatatt gacagaaaca
    16621 actgattata aaaaatattt agacccttcc gttatttccc ggctttctaa catggaactc
    16681 aaagcccgga tggtagtgga agggtttatt gctggtctgc ataaatcacc atatcacgga
    16741 tttagcgttg agtttgcaga gcacagacag tacatgcccg gagatgatct gaaatttatt
    16801 gactggaaaa ttctcggcaa aactgaaagg tattatgtaa aaagatttga ggaagagact
    16861 aatcttaaga cttatctttt actcgatatc agcagatcta tggattttaa atcggaagat
    16921 acttcttctt taaataattt aagaaaaggg ttttttaaaa aatatttctc atccgaaaac
    16981 aaagaagaat tacaaacaag ttctgatctg acaaaacttg aatatgcgtc tcatcttgcg
    17041 gcgtctttag cttatctcat gctgcttcag cgtgatgcca tttctctaac aacttatgat
    17101 acacatataa ggtcatatat tcctcctcat tctacaaagg caaatctcag attaatatta
    17161 aaagagttaa ctaacattaa atctttaaat caaactggaa ctgctaaatg tctgaatgaa
    17221 attgctgata aagttaaaaa aagaggtctt gtaattttgt tcagtgatct ttttgatgaa
    17281 cagaaagatg ttttaaatgc tcttaaacat ttcagataca ataaaaacga agttataata
    17341 tttcagataa tggatcctat ggaaataaat tttctggaag gaagtcctgt aactttaaaa
    17401 gatatggaaa ctaacgaaga gatgtactct cagccggctg caatgcagaa aatttacaga
    17461 gaatctattc aggagttcat tcagaattac aaaactgaat gcattaaaaa taatatagaa
    17521 tttataactt tgtccacagc aacttctttt gagaccgcac ttcttggata tctaaacaaa
    17581 agaaaaagac taatttaata tatttaaaaa attataatta ttaatttctg atcctgttag
    17641 catcagtatt ccagatcgga atagtcagct tataatcacc aaatcctgtt tcatctacaa
    17701 aaatatattg ctgattttca ttataatatt cccagatttc ataaggtttt acgccttcat
    17761 taaaaggatg cctttcgata ttattcggtt ctccaaaaat aataaaaacc atacccatat
    17821 ctgttttcca gccgtctata tagtgtgaat atctttcatt tgcttctcct actcttttat
    17881 aatattccac catcagttca ttcttggttg tataaggcga aggatctttt cccctccaga
    17941 attctacaaa atttttctct ttcaattcag gtgttttaga atttttaatt tctgtgatct
    18001 cttcgctgct tgcaatatag atcatctgat caatcaatat gttcagatcc tttacattag
    18061 tcggcattcc actgagttta ttctcgaatt ctttataagc aactatctcg cctgtactgt
    18121 tattttttaa ctcaattata tattcaccaa aaatcagatt acttgtagaa attttttcga
    18181 aatatttatt tattccggga ctcagagtgt aaactaaatt atccttttcc aatatatttt
    18241 ccttactgtc agtaatagta tatgtgaaat catttacaac ttcattatcg cttgaattat
    18301 atacttcgaa aaataaatag atcgatttca gatttccaat gttcctggca ataagaggtg
    18361 ttattacttt tttaccattc tcttctttta cattgctgac aagcatgata ttacttgcgg
    18421 aaatatcttt ttgcacaaaa tccgtaattt ccacactccc cttctctgtc ttttcttttt
    18481 ttgtattaat atccgttatt gaaatttcaa gactgtagtt tccgggattt aagtaaaatt
    18541 cttttacaat atatttagat gaggattcaa gatttttttg ctcggcttta gaggtggaaa
    18601 cgagatcagt gatattttca ttactcacta tctcattaaa ggaatttgtg atcttaatat
    18661 tgtaagtaat gcttgcatca taatttttag tggcgtaatt ttttttgaac tgaagattct
    18721 ctaacggtat ctcaagatat acgtccaatc ttgccttacc ctcatcctta ctgtaaaata
    18781 ccagcggatc gaaataaaag aaatcccttg aatctatctc tatatttttg tcgctaagct
    18841 gtgaataaga atctttaaat gctaaaactg atatgaaaga aattaatatc agacacatca
    18901 gtttttttaa atcactgctg atttctaatt tgatttgttt tgaatgtttc atggcaaacc
    18961 tctcttatta attttaattt ttttaatatt ttaaactatt tctccaaaga gatcaaactc
    19021 ttcagaatca aatattctta cattataaaa ctcacctgtt tttaaatctt gattgcttat
    19081 ataaatttcc tgatctatct caggtgcatc tctgtaactt cttccaatat aatatccatt
    19141 ttcttttctg tcaaccaata ctttaagtac ttcattattt gaattcatgt tacgttttaa
    19201 agaaatttct ttctgaatat ccagtagatg tttttgtctc gaaagttttt cttttagcgg
    19261 aattctgtcc ggtatttctg ctgctgttgt tccatcctca tgtgaatatg taaaaacacc
    19321 taatcggtca aattcatatt cttttatata ctctgttaat tcaataaaat ctttttcagt
    19381 ttcatccgga tatccgacta tgaaagttgt cctgactgca atccccggaa tttcatttct
    19441 gagcttttct aaaagtcctg tcagagtatt tttagttata cctcttctca ttgatttcag
    19501 tacattatca gtgatatgct gaacgggaat atctatataa ttacaaatat tcggagtatc
    19561 tctgatcact tcaattatat cttcaggaaa tctcgatgga taagcataca ttaatcttat
    19621 ccattcaatt ccatttatat tagacaattc tctgaggact tgtgacaact ctctttttct
    19681 gttaatatca aaaccccagt atgtagtatc ctgaccaatt acaataattt cttttactcc
    19741 cttcgaggca aggatctgag cttccattat tatctgatcg agaggtttgg atctgtgtac
    19801 gccgcgcatt atcggtattg cgcagaaaga acatggatta tcacagcctt cactgatctt
    19861 caggtatgca aaatgcgaag gtgtagttaa gcttctttca ccgagcagtt cacttttata
    19921 atcaccgccg agttctttaa gaattccaat aattgtatgc ggcttatcag ttgcaccaaa
    19981 atatttatct acttcgggaa tatctttttc cagttcatcc ttatatctgt ctgacaagca
    20041 tccggctaca taaacatttt tcaaaagtcc tttattcttt ctgtcgactg ctctcattat
    20101 tgtatcgata gattctttct tagcattttc aataaacccg cacgtattaa tgatcacatt
    20161 ctctgcatca tcttcatctt ctactaattc aatgtcattt gacttaagct gtgacatgat
    20221 gaattcagaa tccacaagat tctttgagca tccgagtgta atcagtttta ctttatcttt
    20281 ttttactttc attaaatttt atctctaaat ataaatatga atttttataa atttattcag
    20341 tcaattttct taaataattt acctaaataa aataaccctc caatccatca tatattttga
    20401 gggatgtaag agggttggtt tttctgaaac taatattttt atgtgcaata aaaaatggta
    20461 taaatcttat ttttttctga aatgccggga gttggcaatt tacaccacgg agacacaaag
    20521 acacaaagaa aaacataaac tcactcccct ttttttcgaa ggtaagggag ggatgggatt
    20581 gaaaagaaaa gctccccaca ctttcttttc caaggtgagg gctgggtagg gtttttagaa
    20641 cggaggctga agttctttga tacattattg attcatgcga ctaataagac acatacaaat
    20701 taattaacac catagacagt tatcgtaaat tgaaactatc aaactggtaa cgaccaataa
    20761 gtatttaaat tatacattga ttgattaatc cacattaaac tttttagaac acaaacatct
    20821 ctcggcgact agctttagtt ttatcaataa atttacttta gtattaccat gaaaataacc
    20881 tcagccaaat gcccctcatg cggcgggaat cttgagattc cggataataa ggattatata
    20941 acctgtcttt actgtgagaa tactgtcagg gtcagggacg ccgttccggt attggaaatt
    21001 gataaggtcg agaatttcgc cgtcgttggt gtttttaaaa aatttgcaga gattcacttg
    21061 aagtttacaa tttgactata acaaattttt taatcaccaa cgactggtat cgtaaagaga
    21121 aactataaag ctgttggtga ccaaaaagtt tttaagttaa tcagtgattg aaaaattcag
    21181 attaaacttt ttggaacacc aacagcggct cgaaattatt tagaaagtat tcagttcgga
    21241 aataacctaa ttccaccaag tgatatttat atattattaa ataaatgtta tcgccgggaa
    21301 tttcttgtat cgtc
//
LOCUS       DAORVQ010000032        21257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_949_length_21257_cov_5.806669, whole genome shotgun sequence.
ACCESSION   DAORVQ010000032 DAORVQ010000000
VERSION     DAORVQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_21257_cov_5.806669"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2568)
                     /locus_tag="PLX80_04450"
     CDS             complement(181..2568)
                     /locus_tag="PLX80_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQY20081.1"
                     /translation="MKFFILQITTLIFTLFLISDSVNSFTQTQFSGDTSSAADTTSSG
                     DSTSITDTVSAAFKPNVNPEVTAVRIENGKIKLDGELKEYEWKKAGFAGNFTEISPGD
                     NIRPEVETKAYVMYDDDNIYFAFECFDDMENIRTSMSDRDNMYSDDWVGPFIDTYGTL
                     KEAYEFYVNPYGVQGDLFWTPNNESSSPDFIFESETKIYKDRWVAEMKIPFKTLKFPD
                     INVQQWRVHFLRNRPRNLRQRIYWSSVSRDNPNFLGQSGTILGIEGVKSGNNLEFLPY
                     VSGSEAATLNDPINSESQLDYKKPEADFGFDVKYGITSNLNLNGTYNPDFSQVEADAP
                     QIDVNNPFALFYEEKRPFFLDGESSFRTNLNYVYTRSINNPIFATKLTGKIGKNLEVG
                     YMNAYDENTPFVIPLEDRSFVLQSNKSSFINIFRLKYDLGGQNYIGGLYTDREYSSDS
                     GFTSDFKGYNRNLGMDFSFNFLKNFYFKGQVTGFAEREISDTSFFDNPTKFGNEKQYN
                     VAFNGEAFTGLSTYFAFNRESEKLGFFTEYNYNSPTARRGVGFLNRNDFHTGYSYIFY
                     NIYLENKLFRRITPDADLELTYNTDGVNKTQYLQTGVTFEFQNGTRAFANYKWINNQN
                     YRNTQLDYINGWNFTIENFANNIIGGGIYYSRSKEVVTFGATPSVGGGNTIRLFSTIR
                     PIDRIYSDFNYEYSQLAETFGGELFYAGWIFSNTTSYQFNRNLFLRLVTQYDSFNKAF
                     TCDPLISYKWNPFTALFLGTNHAFEQIQTSALSNQTELKESSRQIFLKFQYLWQM"
     gene            complement(2863..3816)
                     /locus_tag="PLX80_04455"
     CDS             complement(2863..3816)
                     /locus_tag="PLX80_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HQY20082.1"
                     /translation="MNILNPEYLILSEVVKTALVENKPIVALESTIISHGMPYPQNLE
                     TALNVERTVREHGAVPATIAIINGKIKAGLSAEDLEILANGSSTGQEILKASRRDIPF
                     IISQKLNAATTVAATMIFAEMAGIKIFATGGIGGVHRNAQNTFDISADLTELANSNVA
                     VVSAGVKSILDIGLTLEYLETLGVPVIGYRTEEFPAFYTIESGFKVNFKLNSPEEISD
                     VLQKKWELGLKGGVIIANPIPEEFSMDKKLIDNAIEGALEKAEDKNIKGKDLTPYLLS
                     EIKIITSGKSLESNINLVLNNAGLAAEISVCLCRIFENNRK"
     gene            complement(3797..4762)
                     /locus_tag="PLX80_04460"
     CDS             complement(3797..4762)
                     /locus_tag="PLX80_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQY20083.1"
                     /translation="MKFSVTVIGGINIDLKGIADSENFESDSYPGIVRISPGGVACNI
                     AQNLAQLDVEVTLYGSVGDDHYGNFILDILNKKKVNVGYILKSRELSTSAYLSVSNKS
                     QSLFYAVNNTENSVKHITAEYIRKIKVEILNSSMVVIDTNLSIETLDEIVKLCNELFV
                     PVFVSVVSAEKAAKVNNISGKINFLSLNQSEFVKLFGEYKNNDELLEIINSEKSKKYS
                     VIILKKDKKGVDILDVGENFIDNYEAPEVMIADVCGAGDAFDAGFIFYELYKYYENEK
                     PFGSVNMGVCAAAFALGSYETVSPELSRKKLLNSFENRINEYFKP"
     gene            4832..5038
                     /locus_tag="PLX80_04465"
     CDS             4832..5038
                     /locus_tag="PLX80_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQY20084.1"
                     /translation="MQIVTISSKFQIVIPKDIREKLNLRSWDNFQVLQYGNRFELFIV
                     RDIRKSRGFLKGMNTDFEREDDRL"
     gene            5035..5404
                     /locus_tag="PLX80_04470"
                     /pseudo
     CDS             5035..5404
                     /locus_tag="PLX80_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
     gene            5457..6752
                     /locus_tag="PLX80_04475"
     CDS             5457..6752
                     /locus_tag="PLX80_04475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQY20085.1"
                     /translation="MQTISSSFFIPREGTDFDDLYRRVRIMPLYSFPHTKDLNWRIRE
                     HRNRFFVHIDFDAFYAQVEQRDNINLRGKPVSVGGTGGGKGIVMTASYEARAFGVDTG
                     MSVLEAKRICPQLISLPCYGPKYESIILNLMSALKEFVPEDCIEQYSIDECFADLTGV
                     AKDYFEAVKVAAAIKRKIRELENLTASMGLSYNKTYAKLATKLHKPDGLSVITQENKD
                     KIFSMPANKLWGVGRRNEMRMLMLGIRTIRDLAESNERMLRQEFGVNGLVFRKLARGE
                     DTSEIFRKEKKEKSLNHYHTLSEPIYKTADVRKEIRRIGEFICRKLRSKSLVAGFMHL
                     SVRLENLRYSSDLVRLTSYTNDDREIFNTAASIYNRLHKPTMEMKGRQFGMGVYDLHH
                     DLKERNLNLFENEPALPYHALDKLKSKYGEGVIRVGLEN"
     gene            complement(6769..7080)
                     /locus_tag="PLX80_04480"
     CDS             complement(6769..7080)
                     /locus_tag="PLX80_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQY20086.1"
                     /translation="MNNYYCYILKLNPSFYRNEDWTEKETELVGVHFNYLKDLTNKGN
                     VFLAGRTVNEPMTDKDLGIVIIEAESEDEARGYMENDPAIIGNLMTAEFFEFSLALLR
                     K"
     gene            complement(7101..8375)
                     /locus_tag="PLX80_04485"
     CDS             complement(7101..8375)
                     /locus_tag="PLX80_04485"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY20087.1"
                     /translation="MDDRKHVIVIGAGFGGLQAVKKLSGNKDIRITFIDRKNHHLFQP
                     LLYQVATAVLNPADIAIPTRSLTYKMKNVTVLMDEVTEIDKSNKRIILNDKVLPYDYL
                     VIATGAMTSYFGHSIWKEYTLGLKNLMDALSIRNKLLFSFEEAENHPDRADELLKYII
                     IGGGPTGVELAGSIAELSNSIINDDFRNIDTRKSKIILIEGGSDLLPSFDRKLSVYTK
                     KRLEKRGVTIMLNSRVLNIEKNSVFIKAPDGERTLHGNLIIWAAGVKAVPLNADSGFD
                     VDRQQRIKVNKYCSLNEYPDVFAIGDAADFTDENGKPLPGLSAVAMQQGRYVAKCIIN
                     DIKKKSNEPFRYLNKGNMATIGRMDAVGEVSKFRITGTLAWLLWLFVHLFYQVGFKNR
                     VTILITWIWSYFTFGASARIIQEFAPLTKPED"
     gene            8683..10239
                     /locus_tag="PLX80_04490"
     CDS             8683..10239
                     /locus_tag="PLX80_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20088.1"
                     /translation="MIFIILLIIGIKISYHSGLITDIDPFDESIYLFRGITINPFIKH
                     HIDGFFYYLWYNLISHFTADNFELFFINNNILLVLPGLLIFLILRIIRTDILISFISA
                     AIYLVSSANIFVIPLITKFALCIILLGSIWIYKINQPSDKLLIAVLLSFILIYIRPEF
                     ILSLFLNAGLYVYFIIRSYKAKPDKNYLRKFLPLILIAVIIIFFNPISRHRVNIAYAQ
                     HYAKDVLERNDNSKVSIDDITRPDEIMQRDFLTDYSFSDAAINNPVLFIEHIGFNFFR
                     SFTQIDETFPYIINENDNYTLLKNILYGLCILSLLTMCFFIAVRIKRKSFGTFSLIYI
                     SFVLPPLISVLIFYPRTHYVILIFTFILIYLSYEISSRLNSKSMYKKISFSVTLIFGL
                     LLIFLLPFRADSGSIHLAGCTRLYTVKELNKFDFNENVIFFNVGPGITTFLTNDWKYV
                     SDYMLELPIDEFFYKKNVNLILVDKYFFNHPLIINNKEVERILSDTSYVRFEMFGCDS
                     YLLAKKEILN"
     gene            10435..10764
                     /locus_tag="PLX80_04495"
     CDS             10435..10764
                     /locus_tag="PLX80_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HQY20089.1"
                     /translation="MDRNFITTAFDFEGCKITKNLGVVRGIIVRSRNIFANIGAGFQT
                     LVGGDITLYTELCEKTRVDAFEQMVQHAEELGANAIIGMRYDANEVTSGVTEVLAYGT
                     AVVVEQK"
     gene            complement(10884..12128)
                     /locus_tag="PLX80_04500"
     CDS             complement(10884..12128)
                     /locus_tag="PLX80_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20090.1"
                     /translation="MQLENKTFRLHIISQLFTGIAFGILMLQEVILKKSLNATDFEVT
                     ILIFLTSSAFLFSIYGSEIINRSNNQARTIIIMAVFSRMFLIIIPLFESPAFFVFCLA
                     AMSYIDSLMKPSWNTVFKHNYTNERRSILYSYSSIVYTVAVLVVGTLFGYLLDINYKL
                     YKILFPVAAIFEILSYINLSKLITMGKNLHPYCGVLFSGHINYKLVKEILILPVKNMF
                     RIFRENKPFFRFEIFFFMYGVAFMIASPAVPIFLVETLKLDYSPISIAKGLVFYTSTI
                     LITPLMGRMHGSGNPTKFCGYLFLSLVFYPLLLISIKIFGVDMNIIGTDTLLYITFFI
                     FGILMSGITMSWNLSSIYYAPHSEVANYQAVHITLTGVRGLFAPFIGYIILKVFSIEA
                     TFLVSSFFFLLGGILMLRESRK"
     gene            complement(12163..12579)
                     /locus_tag="PLX80_04505"
     CDS             complement(12163..12579)
                     /locus_tag="PLX80_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQY20091.1"
                     /translation="MPLTVNLNSFSYKKEGIPKDPSGHGGGFVFDCRFIYNPGREEEY
                     KKLTGKDIEVIEFLNSQKEMKDFLKNVFDITDPAIENYIERNFNDIMISFGCTGGQHR
                     SVYAAEQTRKHIMEKYPEVNIVLKHVEIEKGLNFGS"
     assembly_gap    12718..12817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12905..13972)
                     /locus_tag="PLX80_04510"
     CDS             complement(12905..13972)
                     /locus_tag="PLX80_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HQY20092.1"
                     /translation="MNLRDQKGRASQSNLPNRFEKLHVDEDYFDESEFSDPEEENNTV
                     TEFFRDDTKTVLTENNSPDVKAKFSINPYRGCEHGCIYCYARPTHEYLGFSSGLDFES
                     KIMVKHNAPDLLRKEFMKKSWRPQPVFFSGNTDCYQPVERKLKLTRSCLEVFCEFRNP
                     VLIITKNSLIRRDIDILKELSDLNLVKTVISVTTLDPELQRKMEPRTSSPSKRFDTVE
                     FLSSHGILTGVNIAPIIPGLTDEEIPEILKQASERGAAFAGRIILRLPHSVKELFTNW
                     LEKNYPDKSKKILNRVREMHGGELYNNEFGKRMTGEGRYADMIKMIFDTNCKKYGLNE
                     NAVQLSIDKFQRTVSGQGKLF"
     gene            14178..14561
                     /locus_tag="PLX80_04515"
     CDS             14178..14561
                     /locus_tag="PLX80_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20093.1"
                     /translation="MKLFAVILIQVLISGNILYSQVAYDMPENYTSAFTEEGLIKYLS
                     EQESKEKIIGTAVIPFEIQHECFVKLSIFDRTGELYELLIDEYMYPGKYSVIFKPEES
                     KIPEEFRFKLEESGKIYVNSFLIKK"
     gene            complement(14687..15565)
                     /locus_tag="PLX80_04520"
     CDS             complement(14687..15565)
                     /locus_tag="PLX80_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQY20094.1"
                     /translation="MFLIFISAGCNTQSENKILSEERPSNYDLDSRSDKSVQTEGLSA
                     PVTNSSNDEISFENINIFTETERMLIRTGSIKLEADNYNSAEEKLLYLVKENNGYVTN
                     SSSQMNVSNNKQGSITVRVPSDKFDSFLNSIGSAGKVISQNISGQDVTSEYIDLAARQ
                     KTQKELESRLLDLLSSKTANLKDVVEVEEKLSSVRQNIESTEGRMKFLKSQSAYSTLS
                     ISIYEPALLQTTSGGGFFYEMKEAFRNGLQGLTGVFSFLITLIISSVPLLVILTLIIL
                     LIRKFIRSKKVKLQNV"
     gene            complement(15852..17306)
                     /gene="glnA"
                     /locus_tag="PLX80_04525"
     CDS             complement(15852..17306)
                     /gene="glnA"
                     /locus_tag="PLX80_04525"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQY20095.1"
                     /translation="MSEKANIEEEKEKLKKINDVFKLVKEKEIRMIDLRFTDLPGQWQ
                     HLSIPVNKLSESTFYEGIGFDGSSIRGWKNINDSDMLAVPDPSTANVDPFMTTKTLSM
                     ICNVYEPESKKIYDFDPRNIAKKTVEYLKSTGIADTAFFGPEAEFFILDHVAYNINEY
                     SSWYRIDSTEGFWNTGSEKEQNLGHKIRIKEGYSPVPPSDSTNDLRSLMVENLINVGI
                     DVEMHHHEVATGGQAEIDFKYCPLLECADDLMMFKYIVKNTAKSVGKTATFMPKPMFG
                     DNGSGMHTHVSLWKDGQPLFAGDKYAGMSEMALHFIGGILKHAPSLLAFTNPTTNSYK
                     RLVPGYEAPVNLAYSKSNRSAAIRIPMYSNNPKAKRIEFRCPDGTANPYLAFSAILLA
                     GLDGIINKTDPGEPLEMDIYHMDEKELKKLRHAPEDLTVALHNLEEDNDYLKQGGVFT
                     DQIIETWIDYKMKKEVKEVQLRPHPYEFHLYYEV"
     gene            complement(17337..17579)
                     /locus_tag="PLX80_04530"
     CDS             complement(17337..17579)
                     /locus_tag="PLX80_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HQY20096.1"
                     /translation="MISESSKHFQSIVEHSQKLKEVLECHSITGDGSHILKIRTENTT
                     TLEKLLSKIQNWPGVKSSKTSIVLSTHKETLEINIK"
     gene            complement(17652..17774)
                     /locus_tag="PLX80_04535"
                     /pseudo
     CDS             complement(17652..17774)
                     /locus_tag="PLX80_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
     gene            18011..18352
                     /locus_tag="PLX80_04540"
     CDS             18011..18352
                     /locus_tag="PLX80_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM14 family protein"
                     /protein_id="HQY20097.1"
                     /translation="MNIYRIMIANASVLIGLGIFGFMTSGSPTALIAPGIGVILIILS
                     ISVKKGNKTMTHIAVALTFVAFMMFLVTGIMRSNTLVIIMAVFTFFAFIMYIMDFMRR
                     KKERESIENKQ"
     gene            complement(18914..19810)
                     /locus_tag="PLX80_04545"
     CDS             complement(18914..19810)
                     /locus_tag="PLX80_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQY20098.1"
                     /translation="MAEFIQQLINGLSLGSIYALIALGYTMVYGILKFINFAHGEIFM
                     LGAFSGLYLARAFGVDSPDIPMALVILILTMVITSIIGVLTEKLAYKPLRNSSKLTVL
                     ITAIGVSLFLQYTGQLLFGADPKSFPTIIENVSFNILGATVGSNQIVVLISAAFMMLG
                     LRFIVMKTKMGIAIRAVSNNMTAASLMGININNVISFTFIIGSSLAGAAGILYSINYP
                     SIDPLMGLLPGLKAFIAAVLGGIGNFAGASVGGLIIGLVETFTVGYLSPTYRDAIAFA
                     ILIIILLVKPTGLFGKKEIEKV"
     gene            complement(19912..21054)
                     /locus_tag="PLX80_04550"
     CDS             complement(19912..21054)
                     /locus_tag="PLX80_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY20099.1"
                     /translation="MNNLCKLFTLFLIVTSGLVLSSCKEESKNEILIGEFASLTGSEA
                     TFGQSSHNALMLAVEEINSSGGVLGKQIKLITDDTQSKTQEVPTVVQKLINRDKVVAL
                     IGEVASSRSKAGAPIAQQFKIPMISPASTNPEVTMIGDYIFRVCFIDPFQATVMSKFA
                     VNSMKVKKVALLVDQKNAYSTGLADNFKNVFPGMGGEIIEEQKYSAGDKDFKAQLTSI
                     KAKNPEAIFIPGYYTDVNLISIQAREIGLTCPLFGSDGWESEKLTEGKAKDALEGSFF
                     STHVSADDPAPAIQNFIKKYKEKYGSQPDAMAFLSYDAGMILFDAIKKAGDTSGDKIR
                     DQLAKVTDYQGITGLITINSDRNAVKPAVVMEIKDGKFVYKETIAP"
BASE COUNT         6777 a         3731 c         3449 g         7200 t
ORIGIN      
        1 tagattctat taaaagttaa tagttaatag ttaatagtta atagttaata gattatagtt
       61 aatagataat agatttttaa tataagtctt tttaaaaaaa accattttga atcactttca
      121 aaatggtttt ttttttataa aaatttttaa ttcctaattt ttaattattt attgctccat
      181 ctacatttgc cacagatact gaaacttcaa aaatatctgt cttgaacttt ctttcagttc
      241 agtctgattg gataaagcag aagtttgaat ttgctcaaac gcatgattcg ttccaaggaa
      301 taaagctgta aatggattcc atttataact tataagcggg tcacaggtaa atgctttatt
      361 aaaagaatca tattgagtca ctaatctcag gaacagattt ctgttgaatt gatatgaagt
      421 agtattcgaa aatatccatc ccgcataaaa aagttcacct ccaaaagttt ctgcaagctg
      481 agaatattcg taattaaaat cactgtagat cctgtctata ggtctgatag tactgaataa
      541 tcttatcgta tttccacctc ctacagaagg tgtagctcca aaagtcacaa cttcttttga
      601 tcttgaataa taaattcctc cgccaataat attatttgca aaattttcaa ttgtgaaatt
      661 ccagccgttt atataatcca gctgtgtatt tctgtaattt tgattattga tccatttata
      721 atttgcaaaa gctctggtac cgttttgaaa ttcaaaagta actcctgtct gtaaatactg
      781 tgttttatta acaccatcag tattataagt aagttcaaga tctgcatcag gtgtgatcct
      841 tctgaaaagt ttattttcca gatatatatt ataaaatata taagaatagc cggtatggaa
      901 atcatttctg ttcagaaatc cgacccctct ccttgctgtt gggctgttgt aattatattc
      961 agtaaaaaat ccaagttttt ctgattccct gttaaaagca aaatatgtac tcagaccggt
     1021 aaaagcttca ccattgaaag caacattata ctgtttttca tttccaaatt ttgtcggatt
     1081 atcaaaaaaa cttgtgtcgg agatctctct ttccgcaaat cctgttacct gacctttgaa
     1141 ataaaaattc ttcagaaaat tgaaactaaa gtccattccc aaattccggt tatatccttt
     1201 aaaatcagat gtaaatcctg agtccgaact atactctctg tcagtataaa gtccgcctat
     1261 ataattctga ccacccagat catattttaa cctaaatata tttatgaacg aacttttatt
     1321 actctgaaga acaaaacttc tgtcttcaag tggtattaca aatggagtgt tttcatcata
     1381 agcattcata tatccaacct ctaaattttt accaatctta ccggttaatt ttgtagcaaa
     1441 aatgggatta ttaattgatc tggtgtaaac ataatttaga tttgttctga atgaactttc
     1501 cccgtcaaga aagaatggtc ttttttcttc ataaaataat gcaaacggat tattaacatc
     1561 tatctgagga gcatctgctt ccacctgact aaaatccgga ttgtaagttc cgtttaaatt
     1621 aagattggat gttattccat attttacatc aaatccaaaa tctgcttcag gctttttata
     1681 atcaagctga gattctgaat ttattggatc atttaaagtt gctgcttctg agccggagac
     1741 atagggaaga aattcaagat tgttaccgct tttaactcct tcaattccaa ggattgtacc
     1801 tgactgtccg agaaaattcg gattgtccct tgatactgaa gaccagtaaa tcctctgcct
     1861 taaatttctt ggtctgtttc taagaaaatg aactctccat tgttgaacat taatatccgg
     1921 aaattttaat gtcttaaacg ggatcttcat ttcagcaacc catctgtctt tgtatatttt
     1981 tgtttctgat tcaaaaataa aatcaggact ggagctttca ttgtttggag tccagaatag
     2041 atcaccctgc acaccgtatg gatttacata aaattcatat gcttctttca acgttccgta
     2101 agtatcaata aaaggtccca cccaatcatc tgaatacatg ttatccctgt cagacataga
     2161 agttctgata ttttccatat catcaaaaca ttcgaatgca aaataaatat tatcatcgtc
     2221 atacatcaca tatgcctttg tttccacttc tggtctgata ttgtctcccg gactgatctc
     2281 agtaaaattc ccagcaaatc ctgccttttt ccattcatac tctttaagct ctccgtccaa
     2341 ttttattttg ccattctcaa tccttactgc agtaacttcg ggatttacat taggcttaaa
     2401 tgctgctgaa actgtatctg taattgatgt cgaatcaccg gatgatgtgg tatcggcagc
     2461 agatgaagta tcacctgaaa actgagtttg tgtaaatgaa tttacagagt cagaaattaa
     2521 aaataatgtg aatattaagg ttgtgatttg aagtataaaa aacttcatgg tgtaaaattt
     2581 ttatttatat aaatattttt tgattaatgt ttttaaaatt ctttacatcg attaaactaa
     2641 atttcattga ttctaaagaa tgtaaaacta aaatattaaa acttataaaa aaaatgaatc
     2701 tcacacttat atggtttttt agtaaccaat ttttttactt taattttata taagtgatta
     2761 tataaggtgg atttgttacg acatattaaa aaaataagtt tcacgaccgg ggaactttat
     2821 taaatttata atttctttac ggtctgtttc atttttaaaa gttcattttc tgttgttttc
     2881 aaagatccta cacaaacaga cagagatctc agcagctaaa cccgcattat tcaatacaag
     2941 atttatattg gattccagac tttttccact tgtgattatt ttgatttctg acaggaggta
     3001 tggagttaga tctttacctt taatattttt atcttcagct ttttccaatg cgccctcgat
     3061 cgcattatca attaattttt tatccattga aaattcttca gggattggat tagcaattat
     3121 aacaccgcct ttgagaccaa gctcccattt tttttgtaaa acatccgaaa tttcctcagg
     3181 tgaatttaat ttaaaattaa ctttaaatcc tgattctatt gtatagaagg caggaaattc
     3241 ttcagtcctg taaccaatca cgggtactcc aagtgtctca agatattcta gagtaagtcc
     3301 aatatctaat attgacttca caccggccga tactactgct acatttgaat ttgcaagttc
     3361 agtcaaatct gctgaaatgt caaaagtatt ctgcgcattt ctgtgaactc cgccgatccc
     3421 gccggttgca aatattttta ttccggccat ctcagcaaag atcatagtag ccgccaccgt
     3481 agtagctgcg tttagctttt gagaaatgat aaatggaata tctcttcttg atgctttgag
     3541 aatttcctgt ccggtagaac ttccatttgc caatatctca agatcctctg ctgatagtcc
     3601 cgctttgatc tttccattta ttatggcaat tgtagcaggc acagctccgt gctctctaac
     3661 tgttctttcc acatttaaag cagtttcaag attctgcgga taaggcattc catgagaaat
     3721 tatcgttgac tctaatgcga caattggttt gttttccaca agtgccgttt ttactacttc
     3781 ggataatatt agatattcag ggtttaaaat attcatttat tctattttca aaagaattta
     3841 ataatttttt tctgctgagt tcaggtgaaa cagtttcata agaacctaaa gcaaatgctg
     3901 cagcacaaac tcccatattt actgatccaa acggtttttc attttcataa tatttatata
     3961 attcataaaa tataaatccc gcgtcaaaag catctcccgc cccgcaaaca tctgctatca
     4021 taacttcagg tgcttcataa ttatctataa aattttcacc gacatcaaga atgtccacac
     4081 cttttttatc cttttttaaa attattaccg aatacttttt tgacttctca ctattaataa
     4141 tttcaagcag ttcatcatta ttcttatatt ctccaaaaag ctttacaaat tctgattgat
     4201 tcaatgacag gaaatttatt ttacccgaaa tattatttac ctttgcagct ttctcggcag
     4261 agaccactga gacaaacacg ggcacaaaaa gttcattaca caattttact atttcatcta
     4321 atgtttcaat ggagagattt gtatctatta ccaccattga actattcaag atttccactt
     4381 taattttcct gatatattca gccgtaatat gctttacaga attttcagta ttattaactg
     4441 cataaaacaa gctctgactt ttatttgaga cagatagata agcagacgta cttaactctc
     4501 tggattttaa aatatatccg acgtttactt tctttttatt aagaatgtct aaaataaaat
     4561 taccataatg atcatcaccg actgaaccgt acagagtgac ttcgacatca agctgagcca
     4621 gattctgagc aatattacaa gccacaccgc cgggcgagat cctgactatg cccggataag
     4681 aatccgattc aaaattttca gaatctgcga tcccttttaa atcaatattg attccaccta
     4741 tgacagttac agaaaatttc ataaaaaatt taatcaaatt tagattgata aaataaaact
     4801 ttataagatt aattgcagtg ggtatatcga tatgcaaatt gtaacaatat cttcaaaatt
     4861 tcaaatagtt attccaaagg atatccgtga aaaattgaat ctcagatcct gggataattt
     4921 tcaggttctt cagtatggta acagatttga attatttatt gtcagagaca ttagaaagtc
     4981 acgtggtttt ttaaaaggaa tgaatacgga ttttgaaaga gaggatgaca ggctgtgaat
     5041 ttaactgaca gttcgggctg gctggagtat tttaccgatg ggaaatgtgc aaaacattat
     5101 gctccagtaa ttgagaataa cgaagattta attgtatcta ctttaaatct ttatgaagtt
     5161 ttcaaaaaga tcttaaagga aaaaaatgag gatttagcaa agaagcaatt ggaataatga
     5221 tacagtctca agtaattgaa gttgacattt ctatagctct gagtgctgca aagttaaatg
     5281 cgatttgaaa atacctgtgg ctgacagcat tatatatgca actgcacgag cttataatgc
     5341 tgtcgtttgg acacaggatt atgactttgc agatctgtca gacgtaaaat tcatcaaaaa
     5401 ataactcccg gttttttccc ctttaaattt aattttatac catatattat tatggtatgc
     5461 aaacaatcag ctcttcattt tttattcccc gcgagggaac ggatttcgat gacctctaca
     5521 ggagagtgcg catcatgccg ctttattcat ttcctcatac gaaggatctg aactggagaa
     5581 tacgcgagca ccgtaacagg tttttcgtgc atatcgactt tgatgcattc tatgcgcagg
     5641 tcgagcagcg tgataatata aatctccgcg ggaagccggt ctcagtcggg gggacaggcg
     5701 gcggtaaagg gatcgtgatg acggcttcct atgaggcgag agcttttggg gtcgatacgg
     5761 gaatgtctgt actcgaagca aagaggatct gcccgcagct aatctcactg ccgtgttacg
     5821 gtccgaaata cgaaagcatt attctcaatc tgatgagcgc tctgaaggaa ttcgttcccg
     5881 aagactgcat tgagcagtac agtatcgatg aatgttttgc agatctgaca ggcgttgcaa
     5941 aggattattt cgaagctgta aaagtggcgg cggctattaa acgtaagata cgcgaactcg
     6001 aaaacctcac tgcgtcaatg ggattatcat ataataaaac ttatgcaaag cttgctacaa
     6061 aacttcataa gccggatggg ctttccgtga tcactcagga gaataaggat aagatatttt
     6121 caatgcctgc gaataaacta tggggagtcg gaaggagaaa tgagatgcgc atgctgatgc
     6181 tcggtatcag gacgatccgc gatcttgcag aaagcaatga aagaatgctg agacaggaat
     6241 tcggagtgaa cggacttgtc ttccgtaagc ttgcccgtgg agaggatacc tccgaaattt
     6301 tccgcaaaga aaaaaaggaa aagtcgctga accattacca tacactttcc gagccgatct
     6361 acaaaactgc tgatgtaaga aaagagataa gaaggattgg cgagtttatc tgccgaaagc
     6421 tgcgttctaa aagtctcgta gcgggattca tgcatctgtc ggttcgtctg gaaaatctga
     6481 gatattcatc cgatcttgtg aggctgacaa gttatacaaa tgacgacagg gaaatattca
     6541 atactgccgc ctccatttac aaccgtcttc acaagccgac aatggaaatg aaaggacggc
     6601 agtttggaat gggtgtgtac gatcttcatc acgatctgaa agaaagaaat ctcaatcttt
     6661 ttgaaaatga gcctgcactc ccttaccacg cacttgacaa gttgaaatca aaatatggtg
     6721 aaggggtgat acgggtaggg cttgaaaact gaatttccgg aagaaaattt atttacgcag
     6781 cagcgcaaga gagaattcaa aaaattccgc agtcattaaa tttccaatta tagcaggatc
     6841 attttccata tatcctctgg cttcatcttc agattcagct tcgatgatta caataccaag
     6901 atctttatca gtcatcggtt cattcacagt cctgcctgca agaaaaacgt ttcccttatt
     6961 tgtcaggtct ttgagataat tgaaatgcac tcctacaagt tcagtctctt tttcagtcca
     7021 gtcttcgttt ctgtaaaatg aaggattgag tttcaggatg taacaataat aattattcat
     7081 taaaaaaatt ttaaattaag ttagtcttcc ggttttgtaa gtggggcaaa ttcctgtatg
     7141 attcgcgccg atgccccaaa tgtaaaataa ctccatatcc aggtaataag tatagtcact
     7201 ctgtttttga atcctacctg ataaaataaa tgaacaaaca gccacagcag ccacgccaac
     7261 gttccggtga tcctgaattt agaaacttca ccaactgcat ccatcctgcc gatagtcgcc
     7321 atgttacctt tattcagata tctaaaaggc tcattgcttt tcttcttaat atcattgatg
     7381 atacactttg ccacatacct gccctgctgc atagccactg ctgataaacc cggaagcggt
     7441 ttgccgtttt catcagtaaa gtcagccgca tcaccgattg caaatacatc gggatactca
     7501 tttaaagagc agtatttatt tactttaatt ctctgctgtc tgtccacatc aaatcctgag
     7561 tctgcattaa gtggtacagc ttttacacct gctgcccaga tgattaaatt tccatgcaaa
     7621 gtcctttcac catccggtgc tttaataaat acagaatttt tttcaatatt gagaacccgt
     7681 gaattcagca ttatggttac tcctcttttc tcaagccgct tttttgtata gacggaaagt
     7741 ttcctgtcaa aagacgggag cagatcagaa cctccttcga taagtatgat ctttgatttt
     7801 cgtgtgtcaa tatttctgaa gtcatcatta ataatgctgt ttgaaagttc tgcaatagaa
     7861 cctgcgagtt ccacacctgt aggaccgccg ccgattataa tatattttaa aagttcatct
     7921 gcccggtccg gatgattttc cgcttcttca aacgagaaga gaagtttatt tcgtattgac
     7981 aatgcatcca ttaaattttt cagaccaagt gtatattctt tccagatact atgtccaaaa
     8041 taacttgtca ttgcacctgt tgcaatgacg agataatcat atggcaaaac tttgtcatta
     8101 agaataattc ttttattcga tttgtcaatt tctgttactt catccataag aacagttacg
     8161 ttcttcattt tataagtaag agatctggta gggatggcaa tgtcagccgg atttaatact
     8221 gccgttgcaa cctgataaag cagcggctga aagaggtgat gattttttct gtcaataaat
     8281 gtgatccgga tatctttatt acctgaaagc ttttttaccg cctgcaatcc tccaaacccc
     8341 gcaccgatca ctatcacatg ttttctgtca tccataagta aaatttaatc attatattat
     8401 tactgtagtt tgattcatgt aaaaataagg tttatgcatt aatgatttaa atgaaaaata
     8461 aatactttag taaaagttag aatgcatcct taattcaaag atttctcagc ttcgtcctat
     8521 ttgctttttc catttaatta caatttgaaa tttgtaattt gcaataaaat tcaacaggta
     8581 tattttttaa aatatctata taagtatgta ccaatgaata attcaactat agcaaatatt
     8641 aaataaggat tgaatactct taaaaaatat tttccggatt gtttgatttt tatcatcctt
     8701 ctgataatcg gaataaaaat ttcatatcat tccggactca ttacagatat agatccgttt
     8761 gatgaatcca tatatctatt ccgcggaatt acgataaatc catttataaa acatcatatc
     8821 gacggatttt tttactatct gtggtataat ttaatatcac attttacggc agataacttc
     8881 gagttatttt ttataaacaa taatattcta ctcgtacttc ccggtctttt gatatttctt
     8941 attttaagaa taattagaac agatatactg atatctttta tttcagcagc gatatatctt
     9001 gtttcatctg ctaatatttt tgtgattcct ttgattacaa agtttgcatt atgcatcatc
     9061 cttttgggat caatatggat ttataaaatt aatcaaccaa gtgacaaatt acttattgct
     9121 gttttattat catttattct gatatatata agaccggagt ttatcctgtc tctcttttta
     9181 aatgcgggtt tgtatgttta cttcattata agaagctaca aagcaaagcc tgacaaaaat
     9241 tatctaagaa aatttttacc gctgatactg atcgccgtta ttataatttt tttcaaccct
     9301 atatcaagac accgtgtaaa cattgcctat gcccagcatt atgcaaaaga cgtgcttgaa
     9361 agaaatgata attcaaaagt aagtatagat gatattacaa ggccggatga gataatgcag
     9421 agagattttc tgactgacta ctcattttca gatgctgcaa taaataatcc cgttctgttt
     9481 attgaacata tcggatttaa tttttttagg agcttcacac agatcgatga gaccttccca
     9541 tacattatta atgaaaatga taattatact ttattaaaaa atattctgta tggattgtgt
     9601 atattatctt tgttaacgat gtgctttttc atagctgtaa gaattaaaag aaaaagcttt
     9661 ggaactttca gtttgatata tatatcattt gttttgcctc cgcttatttc ggtattaata
     9721 ttttacccaa ggacacatta tgtaatatta atttttactt ttattttgat ttatctgagt
     9781 tatgagattt catcaagact aaattcaaaa agtatgtata aaaaaatttc gtttagtgtt
     9841 actttgatat ttggtttact gttaatattt ttattgccat tcagagcaga ctccggttct
     9901 atccatcttg caggatgcac aaggctttac acagtaaagg agctcaacaa atttgatttc
     9961 aatgagaatg taattttttt taatgtcggt ccggggatta ctacatttct gactaatgac
    10021 tggaaatatg tcagtgatta tatgcttgaa ttaccaatag atgaattttt ttataaaaaa
    10081 aatgtcaatc tcatattggt agataaatat ttttttaatc atcccttaat aataaataat
    10141 aaggaagttg aaagaatatt atctgatacc tcatatgtca ggtttgaaat gtttggatgt
    10201 gattcctatc tgctggctaa aaaggaaata ttaaattaat tcataattat agaaataaaa
    10261 gattttgtga ttttcacttg atagttccgg agaatatgat atttgcatta attaattttt
    10321 tatattcagc accggcgatt aaaatctaca ttcatttcaa agccaattct cctcttgaaa
    10381 aaacttttca ataagcgtat tttaaacaca ttaataattt tataaatatt tcaaatggac
    10441 agaaatttta tcacaactgc ttttgatttc gaaggatgta agatcacaaa aaatctcggg
    10501 gtggtcagag gaataatagt tcgctcgaga aatatctttg caaatatcgg tgccggattt
    10561 caaacacttg taggcggaga tattacgctt tatacagaac tttgcgaaaa gacaagagtg
    10621 gatgcattcg aacagatggt gcagcatgct gaagagcttg gagctaatgc aattataggg
    10681 atgagatatg atgcaaatga ggtgacctca ggagttacag aagtacttgc atatggaact
    10741 gcggttgtcg tggaacaaaa atgaagtaag atatcaatgt acaatgtata atgtgcaatg
    10801 ccatatgttt aataatcatt ttctaaaatt gtaattcgta acttttaatt cgaaacttgt
    10861 aatacgtaac tcttaattcg taattatttc ctgctttcac gaagcatcaa aattccgcca
    10921 agtaaaaaga aaaatgaaga gactaaaaat gttgcttcaa tcgaaaaaac cttcagaata
    10981 atgtaaccga taaaaggagc aaataaacct ctgacacctg tgagtgtaat atgaactgcc
    11041 tgataatttg cgacttccga atgcggtgca taataaatgg aactgagatt ccatgacatt
    11101 gtgataccgc tcatcaaaat tccaaatata aaaaatgtaa tatataaaag tgtatcagta
    11161 cctataatat tcatatctac accgaatatt tttattgaaa ttaacagcag cggataaaag
    11221 acaagtgaaa gaaaaagata gccgcagaat tttgtaggat ttccagaacc atgcatcctg
    11281 cccatcagag gtgtaatcaa aatagtagaa gtatagaaca ccagtccctt tgcaatcgag
    11341 atcggcgaat aatctaactt aagtgtttca actaaaaaga tcggaacagc aggagaagct
    11401 atcataaatg cgactccata catgaagaag aaaatttcaa atctgaaaaa tggtttattc
    11461 tctctgaata ttctgaacat attttttacc ggtagtataa gaatttcttt taccagtttg
    11521 taatttatat gtcctgaaaa taaaactccg cagtagggat gcaaattttt acccatcgtt
    11581 ataagctttg acagatttat ataagataaa atttcaaata tggcagctac gggaaataat
    11641 attttataga gcttataatt tatatccagc agatatccaa acagagttcc cactacaagt
    11701 actgcaactg tataaactat ggaggaatag gaatataaaa tactccttct ttcattagta
    11761 taattatgtt tgaataccgt attccatgac ggcttcatta gtgagtcgat gtaactcatt
    11821 gcagcaagac agaaaacaaa aaatgcgggc gattcaaaca gtggtattat gataagaaac
    11881 attctgctga agacagccat gataattatt gtcctagcct gattgtttga gcggtttatt
    11941 atttccgatc cgtatattga aaaaagaaat gcagagcttg taagaaatat cagaattgta
    12001 acttcgaagt cagttgcatt cagagatttt tttagaatga cttcctgaag cataagtatg
    12061 ccgaatgcaa tgcctgtaaa caattgagaa attatgtgaa gtctgaaagt tttattttca
    12121 agctgcatga cctctgcaga atgatagact tttattttct ttttagctgc cgaagttcag
    12181 acctttctca atttcaacgt gttttaatac aatgtttact tcgggatatt tttccattat
    12241 atgtttccgg gtctgctctg cagcatatac agaccggtgt tgtcctcctg tgcagccaaa
    12301 tgagatcatt atatcattga aatttctttc tatataattt tctattgcag ggtcagtaat
    12361 atcaaatacg tttttaagaa aatctttcat ttctttttgt gaattcaaaa attcgataac
    12421 ctcaatatct ttaccggtaa gttttttata ttcttcctca cgacccggat tgtatatgaa
    12481 ccgacagtca aatacaaatc cgccgccgtg acctgaagga tcttttggaa ttccttcctt
    12541 tttatagctg aatgaattta agtttacggt taatggcata aataatatta atagaattat
    12601 atcaaatata tcaaactgaa aattcaaatt aagtaaagaa ataggaaaga attgttgtta
    12661 aaattcttat tgatccaaaa acatttaaaa taaaatttaa tatttcccga ttctaatnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa tattaaattt catttttgaa
    12841 tattcatcaa attaataatt ccatttcaaa acattatggg cagaggtatt tacaaagatt
    12901 taaattaaaa cagcttcccc tgcccggaaa cagtccgttg aaatttatca attgaaagct
    12961 gaacagcatt ttcattcaga ccgtattttt tacagttagt atcaaatatc atttttatca
    13021 tatctgcata ccttccttca cctgtcattc tttttccaaa ttcattatta taaagctctc
    13081 cgccatgcat ttctcttacg cggttcagga tctttttaga cttatccgga taattttttt
    13141 caagccaatt tgtaaacagc tctttaacag aatgaggcag tctcaaaatt attcttcctg
    13201 caaatgccgc acctctttca gatgcttgct tgagaatttc tggaatctct tcatcggtca
    13261 gtccgggaat aatcggagct atattcacac ctgtgagaat tccatgtgaa gacaggaatt
    13321 caactgtatc aaatcttttt gaaggggaag atgttcgagg ttccattttt ctctgaagtt
    13381 caggatcaag tgttgtgact gatattacag tcttcaccag attgagatca gaaagttctt
    13441 ttaaaatatc aatgtctctc cttatgagag aattttttgt aatgatcaga acaggatttc
    13501 tgaattcaca aaatacttca agacagcttc tcgttaattt aagttttctt tctaccggct
    13561 gatagcagtc agtatttccc gaaaaaaata ccggctgagg tctccagctt tttttcataa
    13621 actcttttct caaaagatcc ggagcattat gctttaccat tatcttggat tcaaaatcca
    13681 gccctgacga aaatccaaga tactcatgtg taggtcttgc ataacaataa atacaaccgt
    13741 gttcgcaacc tctgtatgga ttaatactga actttgcttt tacatccgga ctgttgtttt
    13801 cagttaaaac ggttttagta tcatccctga aaaattcagt aacagtatta ttttcctctt
    13861 ccggatcaga aaattcactt tcatcgaaat aatcttcatc aacgtgaagc ttttcgaatc
    13921 tgtttggaag attggattgt gatgcgcggc ctttttgatc tcttaaattc atttgtataa
    13981 tttaaataca tacccataaa atattaaggt aaatatttat atataatttg ttgtaaaaat
    14041 aatttcagac ttttctttaa gtaattatac ttatgaatat tgcgtattca cttctattca
    14101 aaaaagaaag cattatttcc atttttgtat agatcaatta aatgactaac caattaacta
    14161 acaataaaaa attttatatg aaattatttg cagtaatatt aattcaggtt cttatttcag
    14221 ggaacatact ttattcacag gtggcatatg atatgcctga aaattacact tctgctttta
    14281 ctgaagaagg acttattaaa tatttgtctg aacaggaaag caaagaaaaa ataattggca
    14341 cagcagtgat tccatttgaa attcagcatg aatgttttgt aaagttgagc atctttgaca
    14401 ggactggcga gttgtatgaa ttgctgatag acgaatatat gtatcccgga aaatactctg
    14461 taatctttaa gcctgaagaa agtaaaattc cggaagagtt tagatttaaa ctggaggagt
    14521 ccggaaagat ttatgtaaat tcttttctga tcaagaaata gattctattt actaatcggt
    14581 aaataaattg gcgcatatcc ccaaatgtga accaaccctc aggttaacag gacagaattt
    14641 ctgtcctgtt tcctttgagg attttttatt ttaaagataa gttaatttaa acattttgaa
    14701 gcttgacttt ttttgagcgg ataaattttc ttatcagtaa tattattaaa gtaagtatta
    14761 ccaataatgg aacggaggaa attatcaatg ttatcaaaaa gctgaatact cctgttaatc
    14821 cctgcaggcc atttctaaat gcctctttca tttcataaaa aaatcctccg ccggaagttg
    14881 tctgaagtag tgcaggctca tatatagaaa ttgaaagtgt agaatatgcc gactggcttt
    14941 ttagaaattt cattctgcct tctgtacttt ctatattttg tcttactgaa gaaagctttt
    15001 cctctacctc caccacgtct ttcagatttg cggttttact gcttagcaga tcaagaagtc
    15061 tggattcaag ttccttctgt gttttttgtc tggctgcgag atctatgtat tctgatgtca
    15121 catcctgacc ggaaatattc tgtgagatca cttttcctgc tgagccaatg ctgtttagaa
    15181 aactgtcgaa tttatccgaa gggacacgca cagtaatact gccctgttta ttatttgaca
    15241 cattcatctg tgaagatgaa ttagtcacat atccgttatt ctctttgact aaatataata
    15301 acttttcttc agcagaatta taattatctg cttctaactt tatactgcct gttctgataa
    15361 gcatcctttc agtttcagta aaaatattaa tattttcaaa actgatttca tcgtttgatg
    15421 aatttgtaac aggagctgac aaaccttcag tttgaacgga tttatcagag cgtgaatcta
    15481 agtcataatt tgaaggacgt tcttcagaaa gtattttgtt ttcactttga gtattgcagc
    15541 ctgctgaaat gaaaattaaa aacataattg caaagatgac tcttgctttg atttttgttt
    15601 ttataacttt aaattttagg tttagaaaag ttttgtaaag ttaaatatga aaattatgaa
    15661 tttgttccga ataatttttt cttaatactt tacaaaaaaa atccccgacc atttgaaaat
    15721 gatcaggaac aataaattta ttacataaat aaatcaataa gttaatttct atcgaatttg
    15781 taaagaaata aaaaaaactt ccgaaaaatg ttattcttca gaagtttttt aaatgataag
    15841 tctatataaa tctaaacttc ataataaaga tgaaactcat aaggatgcgg tctgagctga
    15901 acttccttaa cttccttttt catcttataa tcaatccacg tctctatgat ctgatcagta
    15961 aagactcctc cctgtttcag ataatcatta tcttcttcaa gattatgaag cgctacagta
    16021 agatcttccg gagcatgtct gagttttttc aactcctttt catccatatg ataaatatcc
    16081 atctcaagcg gctcacccgg atctgtcttg ttaataatac cgtcgagacc tgcaagaagt
    16141 attgcagaaa atgcaagata aggatttgcc gttccgtcgg gacatctgaa ttcaattctt
    16201 ttagcttttg gattattaga atacatcggt atcctgatcg ctgcacttct gttgcttttt
    16261 gaatatgcaa gattcacagg agcttcatat ccgggaacaa gcctcttgta agaattcgta
    16321 gtcggattag taaatgcaag taacgacggc gcatgtttca gaattccacc gatgaaatgt
    16381 aatgccattt cactcatccc tgcatattta tcacctgcaa aaagcggctg accatctttc
    16441 cataaactca cgtgtgtatg cattccgctg ccgttatccc cgaacatcgg cttcggcata
    16501 aatgtagcag tctttccaac tgacttagca gtatttttta caatgtattt aaacatcata
    16561 agatcatcag cacattcgag cagcgggcaa tatttaaaat cgatctctgc ctgaccgcct
    16621 gttgctactt catgatgatg catctctaca tcgatgccga cattgattag attttctacc
    16681 atgagacttc gcagatcatt tgtcgaatcg gatggaggca cgggagagta gccttcttta
    16741 atacggatct tatgtccgag attctgctct ttttcacttc cggtattcca gaatccttcg
    16801 gtcgagtcga tcctgtacca gcttgaatat tcatttatat tataagcgac gtgatcgaga
    16861 ataaaaaatt ctgcttccgg tccgaaaaaa gctgtgtctg ctatgcctgt tgacttcaga
    16921 tattcaacag ttttctttgc aatattcctc ggatcaaaat catatatttt tttagactcc
    16981 ggctcataga cattgcagat catgcttaga gtctttgtag tcatgaacgg atctacatta
    17041 gcagtagatg gatcaggaac tgcaagcata tccgaatcat taatattttt ccagcctctg
    17101 atagacgagc cgtcaaagcc gatgccttca taaaaagtag attcacttaa tttatttacc
    17161 ggtatcgaaa gatgctgcca ctgtcctggc agatctgtaa atctgagatc gatcattctt
    17221 atttctttct ctttgaccag cttaaataca tcattgatct ttttaagctt ttccttttct
    17281 tcttcgatgt ttgctttttc tgacattttt ttatttagtt attttatttt ttgaatttat
    17341 ttaatattaa tctcaagtgt ttccttatgt gttgaaagta ctatgctggt cttggatgat
    17401 tttactccgg gccagttctg aatcttcgat aaaagctttt ccagtgttgt tgtattctcc
    17461 gtcctgatct ttaatatatg tgaaccgtca ccggtaatgg agtgacattc taatacttct
    17521 ttcagtttct gactgtgctc gactatgctt tgaaaatgtt ttgaagattc gctgatcaca
    17581 aaaataaaac atgtaatatc ctgtttcagc tttttgtgat ttaaaattgc atgataggat
    17641 tttatataat catgagattc taatttcgaa attctgtcga tagtggatgg aagggacaga
    17701 cctattattt ccgccagttc atttttcttt atctgcgaat ttttctgaag ctcagaaagt
    17761 atctttatat ctatctgatc aagcttcaga tcatcagtat tttttattgt tgttgaaata
    17821 ccttattaat ttaggaatta tataaaattt acctaacaaa ataaggcata tggtttattt
    17881 tgtcaagtaa gttttaaaag tatgttatac aggtagtaga aaagtggatt tttattaaaa
    17941 gcgtatcttg ttaacttgca actcaataca atttttacaa ctcaatcaaa attacttaaa
    18001 taaaatcaat atgaatattt acagaataat gatcgcaaat gcatctgtcc tgatcggact
    18061 tggtatattt ggatttatga cttcaggcag tcctacagca cttatcgcac ctggcattgg
    18121 cgtgatcctg atcatacttt ccatttcagt aaagaaggga aataagacta tgactcacat
    18181 tgcagtcgct cttacattcg ttgcgtttat gatgtttcta gtgacgggaa ttatgagatc
    18241 aaatacgtta gtaataataa tggcagtgtt tactttcttt gctttcatta tgtatattat
    18301 ggatttcatg agaaggaaaa aggaaagaga atctattgaa aataaacagt aatatatttt
    18361 tgtaagaaaa ccggtttata ccttttcaat ttattattta taaaaattta aggttcattg
    18421 tttctactta ctttcttttg aacgaccaaa agaaagtaac aaagtaaagt cgcccgctga
    18481 tataaaactg ctaaaattct tttcgtaatt gaaataaaat ttccaactca aaatttgttg
    18541 tgcaaatttc tcaaacaaga aaagtttatt tcagcaatac ttaaaaattc tttacagcaa
    18601 ttttacatag gcgggtattt tcaaaaaaat attcaaactt attttttaaa aaatatttta
    18661 aagtacagat ttgagtttgg gtgtcccaat gcggaaaaca ggaaaattta gagattagaa
    18721 taatatattt ggaaaataaa taagatcgca acctaaaggt ttcggctaca tgcctccaag
    18781 ttttaaaatt aaaataaatt ctttgagtct tcgagccttt gaggtaaacc aaaaaatgtg
    18841 aatggaaggc tggaattgaa caaaaaaaag acgcaataaa atacgtcttt aaaattttta
    18901 taaaataaaa tctttatact ttttctattt ctttttttcc aaaaagaccg gttggtttta
    18961 ctaagagtat gattatcaaa attgcaaatg caatcgcatc tctgtaagta ggagataaat
    19021 atcctacagt aaaagtttct acaagtccga tgatcagacc gccgacagat gcaccggcaa
    19081 aattacctat gccgccgagc acagcagcga tgaatgcttt tagtcctggc agcagaccca
    19141 tcagaggatc aatgctcgga taatttatac tgtataaaat tcctgcagcg cctgccagag
    19201 atgaaccgat tataaatgta aatgaaatta cgttgttgat atttattccc atgagcgaag
    19261 cagcggtcat attatttgaa acagccctta tcgcaattcc catttttgtt ttcatcacta
    19321 tgaaccttaa tccaagcatc ataaatgcag cagagataag cacaactatc tgattggatc
    19381 caactgttgc accaagaata ttgaagctta cattttcaat tattgtagga aatgatttcg
    19441 gatctgctcc aaaaagcagc tgacctgtat actgcagaaa aagtgaaaca ccgatcgcag
    19501 ttatcagcac agtaagcttg gatgaatttc tcaaaggttt gtatgccagt ttctcggtaa
    19561 gtactccgat aatggaagtg atgaccattg ttaagatcag tatcaccaaa gccattggaa
    19621 tatcaggact gtccactccg aatgcgcgtg caagatataa tccggaaaat gcaccgagca
    19681 taaaaatctc tccgtgggca aagtttatga attttaatat tccataaacc atagtatagc
    19741 cgagagcaat aagcgcatag atgctgccaa gtgaaagtcc gttaattaac tgctgaataa
    19801 attctgccaa aaaaagattt ttttaatagt taatagttaa tagttaatag ttaatagtta
    19861 atagttaata gaaaaattat ttaaaattta atatttaaaa tttaaaatta attatggagc
    19921 aatagtttct ttatagacaa atttaccgtc tttgatctcc attacgacag cgggcttgac
    19981 agcatttcta tcgctattga ttgtgataag acctgtgata ccctgatagt ctgtaacttt
    20041 tgcgagctga tctctaatct tatctccgct tgtatcaccg gcttttttga tagcgtcaaa
    20101 gagtatcatc cctgcatcat aagaaagaaa agccattgca tcaggctgtg atccgtattt
    20161 ttctttatac ttttttataa aattctgaat tgcaggtgca ggatcatctg ctgaaacgtg
    20221 tgtagaaaag aaactacctt cgagagcatc ttttgcttta ccttctgtga gtttttcaga
    20281 ttcccatcca tcacttccaa acaacggaca agtcagaccg atctctcgtg cctgtattga
    20341 gatcagatta acatcagtat aatatcccgg aataaatatt gcttcaggat tttttgcttt
    20401 aatggaagtt agctgagctt taaaatcttt atctcctgct gaatattttt gctcttcgat
    20461 aatctctccg cccattcccg gaaatacatt tttaaaatta tctgcaagtc ctgttgaata
    20521 agcatttttc tgatccacca gtaatgctac tttttttacc ttcatagaat tgacagcaaa
    20581 cttgctcatc acagtcgcct gaaaaggatc tatgaaacat actctgaaaa tataatctcc
    20641 tatcattgtg acttcgggat ttgtggaagc gggagagatc atagggatct tgaactgctg
    20701 agcgatcggt gcaccggctt tacttcttga tgatgcgacc tcacctatta atgctactac
    20761 tttatctctg ttgatcagct tttgaactac tgtaggaact tcctgtgtct tactctgtgt
    20821 gtcatctgtt ataagcttga tctgtttacc aagtacaccg ccagaactgt ttatctcttc
    20881 gacagcaagc atcagagcat tgtgcgaaga ctgaccaaat gtagcttcac tgccggttag
    20941 tgatgcaaat tcacctatta gaatttcgtt tttactttct tctttgcaag agctcagtac
    21001 taaacctgat gtaacaatta gaaataatgt aaataattta caaaggttat tcatttaaag
    21061 attttagatt aattaaaatt gtttaatata tatcttatat ttattgcccg gataatatga
    21121 atataattgt gattataata gagtcgttta aaataatttt aatagtcaat aaaaacaatt
    21181 cccaattaac tgttaacttt catatctctg tacacgacaa aacttttgaa taaaaaataa
    21241 aaatctgatc caaaatt
//
LOCUS       DAORVQ010000033        21024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_973_length_21024_cov_5.687968, whole genome shotgun sequence.
ACCESSION   DAORVQ010000033 DAORVQ010000000
VERSION     DAORVQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 21024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21024
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_21024_cov_5.687968"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..622
                     /locus_tag="PLX80_04555"
     CDS             410..622
                     /locus_tag="PLX80_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20100.1"
                     /translation="MKAKLLFIAAVISSQILFNYNSYSHPSETPFPKPDTGLEDYDVN
                     NFPGWVYFYKLDAGEFSETKRIILLK"
     gene            657..2159
                     /locus_tag="PLX80_04560"
     CDS             657..2159
                     /locus_tag="PLX80_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20101.1"
                     /translation="MKYLCKIILLLLLQIVLYSTVKSQHSYQWSPERKLTEGYYDSNP
                     DYIRDKFNISFNNNTFETLIFERKNSENDSVSRICVVNIDTSGITGNTVYISSGDFND
                     KNPVICIGDYYNHSIYKHAFAVWESQRDSVTYIVGCYFNINTGWQSPFIIDSSAQCHN
                     PDVASIDSLNYSVVYESSNDIIYKEVNANTGIINYEYNLTDGEMSVCKTPKILENIFN
                     IKKQVTYEKQNTDSTFYLYKRNLNFGNIWSNPETVYGVGDVRNAGFMTTYVDEIPLFE
                     SNQSGYWNIYSLHDNSLITINDFNDSADNTSLQRFFNNLITLDGYIYSDAIAYKENYS
                     GINRLILNSTYASQKDTVIISGDPDKTSMTMSNGIYFQNYDFLIWVIYNKDSSGRSSL
                     YGKSVIVRNTGISETGNILLDRYSLSQNYPNPFNPKTVINYELRVTSDLVSIKVFDVL
                     GNEVSTLVNEKQTPGSYTVTFDGSNFPSGVYFYKLEAGDFAETKRMVLLK"
     gene            2440..3975
                     /locus_tag="PLX80_04565"
     CDS             2440..3975
                     /locus_tag="PLX80_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20102.1"
                     /translation="MKNLITLFISIFIFTTYIQAQELSVKIYDPPVPYYENVEWGSDY
                     VVSDTEPMGRPSGVYKNSASTIYVSVPDTNFSPGLNEGLTILTSTNNGMNWSKIAGIS
                     PAVIITKTKMVRTALDSVYCVFLANGTVRVLNPVSGTLGTFTNYTNIGDFDATTSSSG
                     SIYIIIDLYINNQVRIFGSADGGINWGGGIFLSSNAAHPGISMSATGDTAIVNYYGGP
                     FTDTLSSIIRNVRYRESVPGTLVVEGTFTSPIPAGTPKDQFEAVRYGSNAWLFYTSGI
                     SGSKDINCILSNDGGMTYGAPITIGSMPGRDEFWFDAKYYSLNDGGVGIIYYSDSLQT
                     GPPTNVSDKMYYSSASNSTSSVFSEPIQTSEHPPVYSAIGFIPTLIEYYDTGGDAGAI
                     WVGANGTGNKLYYDRLNNLTGVTQTGIEIPSSYSISQNFPNPFNPNTIINYKIQNSND
                     DLVSLKVFDVLGNEVSTLVNEKQNAGSYSVTFDGSNFSSGVYFYKLEAGNFSKTKSMI
                     LLK"
     gene            3987..4853
                     /locus_tag="PLX80_04570"
     CDS             3987..4853
                     /locus_tag="PLX80_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20103.1"
                     /translation="MKKILLIILIIYSMPGYSYSGFPEYLPLKVGNSWTYSWIEISSG
                     GKFKVTVTKDSVINSKKYYQCNFPGLSQWFRIDSLSGNIYNYSNGGGCAYSPGEILVD
                     SIASNKNDSTNFCGNVKRKCVETGSIELFGNTYAKKEFGPVIVLTADSRKYAENIEIY
                     FMELGDPLPTTYTLLGCVVNGIVYGDTTLTDINQISTKIPEKYSLSQNYPNPFNPKTV
                     INYELRVTIDLVSIKVFDVLGNEVSTLVNEKQTAGSYSVTFDGINFPSGVYFYKLTAG
                     DFIDTKRMVIVK"
     gene            4897..6237
                     /locus_tag="PLX80_04575"
     CDS             4897..6237
                     /locus_tag="PLX80_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20104.1"
                     /translation="MISILKRITILICIFTCTADKSIGQNWILVHDVLIDKSKIININ
                     NERVLFFQTWGITGAPFIGCNDSAIFRTYKLNARTNNTFKPFDGFFDPIICTDFILTE
                     SCIFYRPKEFDVSNQDSNFAIKYFSRMAGVNCTPPELHSVVTNSGNSNNLKATVIRIS
                     PFNDNFIYSLINDSLYKTVNRGISWNYVPGTPGNLRDIILNPYDENYLYLITSQNSIL
                     ISTDNGISFNHSGNNFSSSNTLFFKSSDTIVTFNGTDLYKSSDKGFNWSQLSSLPGNI
                     NCLEFHPSRKEIYYAGIGSVLYVSTNGGINFSVYNNSIPNFHIYGVFKARPELDSIYV
                     VTERGLYLVYDQYITNIRQISTEVPDQYRLFDNFPNPFNPMTIIKYQCTVYNDVSLKV
                     FDVLGNEVSTLVNEKQTAGSYSVSFDGSNFPSGVYFYKLEAGDFAETKRMVLIK"
     gene            6457..7302
                     /locus_tag="PLX80_04580"
     CDS             6457..7302
                     /locus_tag="PLX80_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20105.1"
                     /translation="MNKFILLFTLLAFSVSSFAQKSEVPKYGFYGGLGGNYSFVNFSQ
                     VDANTLGLSEIYINGNKVQTGSAKGPFSITPDSESKLGPTLQVGYFQKFNNSSWLWGS
                     KLSYSYVGTTSTSPVLEIPQFGSNSSGDTFEGVAVVQSFQYSLNHQFSFTPYFGISSG
                     KTFFYLGAGISMSETKEEVNDVVGYAFIQNDTVNISGEPTDFSSTNWVWGFAATAGGT
                     YFLSQSLFLDLSYTFNSTQTQVNDFKAPFSNSKGPFSTSGELIGRTSANEVTNSITLT
                     VNKIF"
     gene            complement(7462..7845)
                     /locus_tag="PLX80_04585"
     CDS             complement(7462..7845)
                     /locus_tag="PLX80_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY20106.1"
                     /translation="MNVINLDKKFKAFSDHFSPKIIAELNGQHVKLGKFIGDFVWHKH
                     DNEDEMFLVIKGKFYMDIKTKEGNEVLQKTLELNEGEMIVIPKGVEHRPHSNEEVQVM
                     LFEPAGTLNTGDVNNAMTKKDPEWI"
     gene            complement(7923..8381)
                     /locus_tag="PLX80_04590"
     CDS             complement(7923..8381)
                     /locus_tag="PLX80_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HQY20107.1"
                     /translation="MSAVSSRKISIIKGISWRIVGTLDTIFLAWLFTGNLNFAIKIGG
                     IELITKIALYYFHERLWNFLKIGNKVLGENNGVKVYTDKHWKSIAKSISWRILGTIDT
                     MIISYFVTGSINRALEIGFTEVFTKMLLYYIHERIWLKFTNRSADKSPHY"
     gene            complement(8735..10420)
                     /locus_tag="PLX80_04595"
     CDS             complement(8735..10420)
                     /locus_tag="PLX80_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20108.1"
                     /translation="MKNFYILIFILMFFLSANVQSQVPERFSKPHDEVEQIFWNKKKN
                     TLSFSDHIPNYNSVLFTNTNISRDSFPQNETSVKISKTDPNRVVAAWRDFRQGVSPAN
                     RRVGYSYSTDGGVTWSVSQLLDSTVFPDFPRNSDPVVAVDTAGNFYIAVITVGQNLAL
                     AIYKSTDGGVTFPISTIVANDGTEDKEWLETDFTPGSPYYNNLYISWTRFSGNSGIKL
                     TRSTNSGSNWSNAVAIADPSSPGQGSDLAIGLDGEIYVTWVGGTATQDYIYFDKSTDG
                     GLSFGTDRVIQQGLTPTIPITSSGVTFPSIDTDVSGGANNGNIYLVWCDGRNGDADIF
                     LTRSTNKGANWSSPLRVNNDPVSNGKMQCWPWLAVSDSGNIAVIFYDTRNTSANNIVE
                     AYLALSTDGGLSFTNTPLSDVPSPTIQPNSDIRFGDYIGIDYYGGKIVPVWTDERTGG
                     FNMECYTAVVDGTVGVSHINNLVPDEFVLSQNYPNPFNPETKIAYSIPVKGHVSLKIY
                     NSIGKEVASLVNRSQNSGNYEVRFDGASLNSGVYFYKIQVNDFVQTKKMILLK"
     gene            complement(10447..10839)
                     /locus_tag="PLX80_04600"
     CDS             complement(10447..10839)
                     /locus_tag="PLX80_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20109.1"
                     /translation="MNWNLILKLSLFGLLMGVLSVFGFIPTGFEFFPWLIIAVISGYL
                     ISKHTTKLVMTQSVVLGLFMGIIVSVFQAVFFDKLLENNPGSLDGFKEIPMSMEPQYV
                     ILFMGPFIGIIFGIVIGLIALGFRKMKK"
     gene            complement(10879..12909)
                     /locus_tag="PLX80_04605"
     CDS             complement(10879..12909)
                     /locus_tag="PLX80_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY20110.1"
                     /translation="MSKIKPIYCYLIIIAVTYFVFNNSLQNGFVFDDESVVVNNPSIQ
                     NADNVLKYFTGEEGFHKVIGKYYRPIVSSSYLMDFSIWGMDPYGFHLTNIIIHIISCL
                     LLFRILSLMFSRYKYRNLFSLFGSLIFAVHPIHTEAVTWICGRTDSIVTMFFFASFLF
                     YILFTGYSHHGTMDKRSDQDNSGNYLYLILSLLFYVFGLLSKEMIVTMPVIILLYDFV
                     YRKKNLQYLKENYLSYLLFIAVTGIYLLIRESVLSGVPDRDSYLYFFNKDTEVVFWTM
                     IKTIPVYFRLLFAPFGLLYHYNGVITDSSSILDFSALLSLIFIFALIGLSVFFYKRDS
                     IISFCILFFLVTLLPVMNIIPTMNLMAERFLYLTSFSLIVLITHLLMLASNKRDFGML
                     IVGVLIIIISLGYMTYLRNMDWKTNETLFSSAIGYEGSVILVNEGNIYSKSKKFDEAK
                     KKYKRAIELRDNNLLAHHNLGLVYMIEGKLDSAEMRISRGIMIDSNAADGYYQLATIY
                     NTLGKNEEAIQLLEKLQTINPNYMDSPSLLENLKNPDSKKDKKDDGKFEISFLQKRSY
                     QYFNEKKYKEAIEDLKKLAELSNDLPSKSGFYNNIAMCYAELKDNDQTEKYFSEAVKI
                     DKNNVNALNGLVTLYLKKNEVSKAMSNLSRIIEINPGDLKAKNTLDSLIKLQ"
     gene            complement(13046..14275)
                     /locus_tag="PLX80_04610"
     CDS             complement(13046..14275)
                     /locus_tag="PLX80_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20111.1"
                     /translation="MTLDEAIRLKTELLIKGLTFSDEALLTVGQGGLEKVFWVFEMTP
                     STHKGTGDGTRPLPNDMLLPHDLYADVRYSPDSPFRVHKSGDAVILLKNGKELCEVRW
                     PKRPDFYDMKTRSGESMKKVASMRGDCGLRVCFDNACTYFAKGEQCKFCNIVPARQRN
                     RDHVVTIKEAEDIYDVVKEAIIDNPVCSHLAMTAGAAKDDGLGNLPKILERLKPLLSE
                     KYFPIVTAITATRNIEETQYLCSTGISSVAFNLEIWDEQIFNEICPGKTRRVGRERWI
                     ESLKEARDLLKPARALSSFVVGLEPMESVLKGIDYLSSEGIFPIMSPFVPMVGTDYEG
                     KEAPDTDWVWDVHEKATEIIHKNLPDAFCDEIWDGDIGICPSCTTTKLFFDFMRRTVP
                     RDNPKRLMSNESVEAFV"
     gene            complement(14321..15550)
                     /locus_tag="PLX80_04615"
     CDS             complement(14321..15550)
                     /locus_tag="PLX80_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20112.1"
                     /translation="MTDRKKTIKLKCDLLINGVRLNELRSDLVETQGWDRDRAVKFLP
                     AEIKLDGNVYVQVRENPEANFRIRISESDLTIEDKVGNVITSGEFVSLPPFSKSYTND
                     LTPFEKVVVYNGMCNLNFTWNYYCDYFRTGTECRFCNLTPAQDFYPDENISNARKTAS
                     QVADVIEKAKKYHTDPRSILTRGTPPDKQGLGGTVQILEELSKRFPYKNDRERTKLLM
                     TISPTKNIDDVKLLYDLGLHSVSYNFEVFDKGYWKAIVPGKDENIGRELWEESLITAV
                     KHFGEGKVFTAMIAGLEPAKTLLEGVHWCCDRGIVPIIVPFSPETGSQFEGFRSPTYK
                     WMFDTHYQAAEIMSEKLPFISTSDYWKYDAPICAECFTGGFLFDIIKENSGINEPHSC
                     CELSKEILNINVPESNT"
     gene            complement(15635..15949)
                     /locus_tag="PLX80_04620"
     CDS             complement(15635..15949)
                     /locus_tag="PLX80_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20113.1"
                     /translation="MSEVKQTSHTIFPPAGSWNNAGEKSHSIFPPAGSWNSASEKSHS
                     IFPPAGSWDNASEKSHSIFPPAGSWDCAAEKSHSIFPPAGSWDNASEKSHSIFPPAGS
                     WK"
     gene            complement(16247..16495)
                     /locus_tag="PLX80_04625"
     CDS             complement(16247..16495)
                     /locus_tag="PLX80_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20114.1"
                     /translation="MAKKVLPTKKAAVKKAAVKKAVVKNAVKKAAVKKAVKKAVVKKA
                     VKKAVVKNAVKKAAVKKAVVKKAVKNAAVKKAVKKALK"
     gene            17216..20743
                     /locus_tag="PLX80_04630"
     CDS             17216..20743
                     /locus_tag="PLX80_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20115.1"
                     /translation="MRKLFILIPVLIIVFFIITGQKNPESEKWSNNFSTNLYDPGVIN
                     SAVLPQVYDFTNPNTETRHVKTPYEVISVSPNIRVLPRTNSYQSEVDIVRHPTNPMIL
                     FGSSNAFNNTGTLFISEGVYVSTDGGITWGGSDTMKLAGGAPVPNQGGDPGITIDKNG
                     TFIQTHLGFSTSGMFGNYSTDNGSTWSSNYTIASGSQDKNLAGTDDSPTSPYYGRSYC
                     VWSLFTSGSPPIDISYTTNGGVSWSTPQQINTPPSGHYSQGCDIRTGPNGEVYVIWAA
                     PVAGVPFTEDFAGFGRSTDGGVNWTVTENAYDMNGIRGIFSSKSSIRVNGFPRIDVDR
                     SGGVRNGWIYTVASEKNISPAGSDPDIIMHRSSNGGTTWSPGIRVNQDPINNGKFQWF
                     PAIRVDEAGGVNIVYYDDRYTQSDSSEVYISRSLDGGNSWSDIQVSDHRFKPQSIGVA
                     GIATGYQGDYIGMTSSNGNLFPIWMDNFSGIYNAWTAPVLISTYPLNSFNLTAPPAGT
                     RISTLQNSTTNFTFDWDTSSSTSSYKWIFGSPTTNPRLLSVNSNTNTLSMTLGQLDNM
                     LSSLGLNQGDSLVGQWDVWAFRNNQTDDSLKAANGSRTITLKRGVPVLSPFSLISPVN
                     NTTILSLNTNSDTVNFSWSNSGQAVNYKWLYASPDFSNLSNIKLRINADNNSFDPSLT
                     LRNSQIDSALAGIGINVGDSSTGQWRVYGYSAGDSLASTQTYNINLRRGFPPTVSSSA
                     DSIIVNVLLGQNFSTNLIIGNTGQFSLDWFISESPAALDNLFIRSEFKIDNELNLQQS
                     NLTKGSADIYHGQDVTDGMGGPDNFGYRWIDSDEPGGPVFDWFEISTIGTQITNWTSG
                     TADDGSVIIPIPFNFNFYGTDYTQLKICTNGWMGFDIASTSNEYNNIAIPSTIEPDNA
                     LFPFWDDHDLRNGGQVFYFNDASSNRFIIEYKDAPHFSTGGPYTYQVMLYSDGRIYFQ
                     YLTMNDPVNSATVGIENSTGTDALQVAFNTPYIHDNLAIKIEKGLHWVTENPVTGIID
                     PGGNQNVNIDFNSSTLSQGIYNGFLQIESNDPATPVKNVKLKFNVGTVGIQNVQTGIP
                     SQYLLAQNYPNPFNPVTNINFSLPKDGLVTLKVYDILGKEVITLVNENKQAGNYRVDF
                     NGSNFASGMYFYKIESGSFVQTRRMILLK"
BASE COUNT         6924 a         3593 c         3462 g         7045 t
ORIGIN      
        1 ttgcgtcttt gtgtctttgt ggtattaatt aaaattatgt tttgagacac tttcggacaa
       61 agggaaggag caaaagtagt ttaataatgc ttttggtttt ggctatgccg gtttatgaat
      121 taaaaattaa gaattgagat ttttgattcc acagttcaca tgcttttcgg actctgtgga
      181 tactacacta aagagagaat atcatttctt tatcaatcct tattcacgca aactcacgta
      241 cttttcgaat tatgcagata ctgatttaaa ataagaatct cattcccctg ttaacttcat
      301 ttaaaaatat ttgtcaacaa tacttctttg acattaaata ttaatcaagt tattttaaaa
      361 gaaatttaag tctgcgtttt ttaagattta ataaaaaatt ggagataaaa tgaaagcaaa
      421 attattattt attgcagcag taatttcatc acagatctta tttaattaca attcttattc
      481 ccatccaagc gaaacaccat tcccaaaacc tgatacagga cttgaagatt atgatgttaa
      541 taattttccg ggctgggttt atttttataa attagatgcg ggtgaatttt ctgaaactaa
      601 gagaattata ttgttaaaat aaaattaaaa atgaatcaaa ataaaattaa aacaatatga
      661 aatatttatg taaaattatt ttattattac ttttacaaat tgtactttat agtactgtta
      721 aatctcaaca ctcttatcaa tggagtccgg aaagaaaatt aaccgaagga tattatgaca
      781 gcaatccgga ttatataaga gataaattta atatttcatt taacaataat acttttgaaa
      841 ctttgatctt cgaaagaaaa aattctgaaa atgattcagt ttcaagaatc tgcgttgtaa
      901 atatagacac atccggaatt accggaaaca ctgtatatat ttcatcagga gattttaatg
      961 acaagaatcc ggtaatctgt atcggggatt attataatca cagtatttac aaacatgcat
     1021 ttgcagtctg ggaaagtcaa agagattctg ttacttatat agtaggatgc tatttcaata
     1081 tcaatacagg atggcaatct cctttcataa ttgactcctc tgcgcaatgt cataatccgg
     1141 atgttgcatc tattgatagt ctgaattatt cagtagtcta tgaatcttcc aatgacataa
     1201 tatataaaga agtaaatgct aatacaggaa ttataaatta cgaatataat ttaacagacg
     1261 gagaaatgtc tgtatgcaaa actcctaaaa ttttagaaaa tatttttaat attaaaaagc
     1321 aggttactta tgagaagcaa aatactgaca gtactttcta tctttataaa aggaatctga
     1381 attttggaaa tatctggagt aatcctgaaa ctgtttacgg agtcggagat gtcaggaatg
     1441 caggttttat gaccacttat gttgatgaga tccctttgtt cgaatcaaat caatccggat
     1501 attggaatat ctattctctt catgacaatt ctcttataac aattaatgac ttcaacgata
     1561 gcgctgacaa tacttcactt caaagatttt tcaataatct gattacttta gacggatata
     1621 tttattctga tgcgattgca tacaaagaaa attattccgg tattaacaga ttaattttaa
     1681 atagtactta tgcttctcag aaagacactg tgataatttc cggagatcct gacaaaacat
     1741 ctatgacaat gagcaatgga atttattttc aaaactatga ttttctgatc tgggttattt
     1801 acaacaaaga ttcttcaggc agaagcagtt tatatggtaa atctgtgatt gtcagaaata
     1861 ccggcatttc tgaaacagga aatattctcc tagatagata ttcgctttcg cagaattatc
     1921 caaacccctt taatccaaaa acagtgatca attacgagtt acgagtgacg agtgatctgg
     1981 tcagtataaa agtctttgat gttttgggaa atgaagtttc gacattggta aatgaaaaac
     2041 aaactcccgg cagctacact gtcacattcg acggaagtaa tttccccagc ggggtttatt
     2101 tctataaatt ggaagcggga gattttgctg agacaaaacg gatggtttta ttaaaataaa
     2161 tttcaaatta caaatgacat attacaaatg acagattaca aaaattttag ggatttaatt
     2221 gtagccgcat gctatacctt gcgcgaattc aacttataaa atattcatcg taaaaaattt
     2281 aagatatatt agggaattaa atttcgagct taataaatgt aaaacaaact gccggcagtt
     2341 attcagtcac attcgactta agtaattttc cgggcggggt taatttatac agaataataa
     2401 tttattttat aaaatactta aacaaaaagg agaattgttt tgaaaaattt aattacactt
     2461 tttatttcca tttttatttt tacaacatat atacaagctc aggaactttc cgttaaaatt
     2521 tacgatccgc cggttcctta ttacgaaaat gtcgaatggg gatccgacta tgtagtatca
     2581 gacacagaac caatgggcag accatcagga gtttataaga attccgcttc tactatttat
     2641 gtctctgttc cagatacaaa cttttctccc ggactgaatg aaggtcttac aatactgacc
     2701 tcaacaaata atggaatgaa ctggtctaaa attgccggga taagtcctgc agtgattatc
     2761 actaagacaa aaatggtcag aaccgctctt gattcagtct attgtgtttt ccttgcaaat
     2821 ggaactgtca gggttttgaa tccggtcagc gggacattgg gtacttttac aaattatact
     2881 aatataggag actttgacgc tactacttca tcttccggaa gtatctatat cataatagat
     2941 ctttatataa ataatcaagt gagaatattc ggttcagcag atggtggcat taattggggt
     3001 ggaggaatat ttctttcatc aaatgctgca catcccggaa tatcaatgtc tgcaaccggt
     3061 gatactgcaa ttgttaatta ttacgggggt ccatttacag atactttgag ttctatcata
     3121 aggaatgtcc gatacagaga atccgtaccc ggaacacttg tagtagaagg tacattcact
     3181 tcgcctattc ctgcaggtac tcccaaagac cagtttgaag ctgttcgata cggatcgaat
     3241 gcctggctgt tttatacttc aggtatttcg ggaagcaaag atattaactg tattttgagc
     3301 aatgacggtg gtatgactta cggagctcca attacaattg gatccatgcc cggcagagat
     3361 gaattttggt ttgatgcaaa atattatagt ttgaatgatg gtggcgtcgg gataatttat
     3421 tattcggata gtttacagac aggtcctcca acaaatgttt ccgataaaat gtattattca
     3481 tcagcttcga attctacttc ttctgttttt tcagaaccaa tacaaacaag cgaacatcct
     3541 ccggtttatt ctgctatagg attcattcct actttgattg aatattatga tacaggcgga
     3601 gatgcaggtg caatttgggt cggcgcaaac ggaacaggaa acaaactata ttacgatcga
     3661 ctgaataatt taacaggagt aactcaaaca ggaattgaaa taccttcatc gtattctata
     3721 tctcagaatt ttccaaatcc atttaatccc aacactatta tcaattataa gatacaaaat
     3781 tcaaatgacg atttagttag tctgaaagtc tttgatgtat tggggaatga agtttcgact
     3841 ttggtaaatg aaaagcaaaa cgcaggcagc tactcagtca catttgacgg aagtaatttt
     3901 tcgagcggag tttattttta taagcttgag gcaggcaatt tttctaaaac taaaagtatg
     3961 attctattaa aataaaatta ataactttga agaaaatact attaatcatt ttaattattt
     4021 attcaatgcc cggatattcg tattcaggat ttccggaata cctgccatta aaagtcggta
     4081 attcctggac ttacagctgg attgaaatat caagcggagg taaattcaaa gttactgtca
     4141 caaaagattc ggttattaat tcgaaaaaat attatcagtg taatttcccc ggtctttctc
     4201 aatggttcag gatagattca ttatccggaa atatttataa ttattctaat ggaggtggat
     4261 gtgcttacag cccgggcgaa attctcgtag actcaattgc atcaaataaa aatgacagta
     4321 caaacttctg cggaaatgta aaaagaaagt gtgtggaaac aggaagtatt gaacttttcg
     4381 gaaacacata tgcaaaaaag gaatttggtc cggtgatcgt acttactgca gatagtagaa
     4441 aatacgcaga gaatattgag atttacttta tggaattggg agatccttta cctacgactt
     4501 atacactttt aggatgtgtt gttaacggca ttgtatatgg tgatactaca ttgacagata
     4561 taaatcagat cagtacaaaa attccggaaa aatattcact ttctcaaaac tacccaaacc
     4621 catttaaccc aaagacagtg atcaattacg agttacgggt tacgattgat ttggtgagta
     4681 tcaaagtttt tgatgttttg ggaaatgaag tttcgacact ggtaaatgaa aagcaaactg
     4741 caggcagcta ctcagtcaca tttgacggaa ttaattttcc gagcggggtt tacttttata
     4801 agctgacggc tggagatttt atagatacaa agagaatggt catagtaaaa tgaaaaattt
     4861 tatcaattta atttataact attggagatt taaaaaatga tttcaatttt aaaaagaatt
     4921 acaattttaa tttgcatttt tacctgtact gcggataaat caatagggca gaactggatc
     4981 ttggttcatg acgtgttaat tgataaatct aaaataataa atattaacaa tgaaagagta
     5041 cttttctttc agacatgggg aataacaggt gctccgttca tcggatgtaa tgactcagca
     5101 attttcagaa cttataaatt aaatgcacgg acaaataaca cattcaaacc attcgatgga
     5161 ttctttgatc ctattatttg tacagatttt attcttactg aaagttgtat tttttacaga
     5221 ccgaaagaat ttgatgtttc aaatcaggat tctaactttg caataaaata tttcagtaga
     5281 atggcagggg tcaactgcac tccacctgaa ttacactctg tagttacaaa ctcgggaaat
     5341 agtaataatc tcaaggcaac tgttattaga atatctcctt ttaatgacaa ctttatatat
     5401 tctttgatta atgattctct gtataaaact gttaacagag gaatcagctg gaattatgtc
     5461 ccgggaacac ccggtaactt acgcgatata attttaaatc cttatgatga aaattattta
     5521 tacttaataa cttctcaaaa ctcaatactg atcagcactg ataatggaat tagttttaat
     5581 cattcgggaa ataacttcag ctcatcaaat acattatttt ttaaaagctc tgatacaata
     5641 gtaacattca acggaacaga tttatataaa tcatctgaca agggttttaa ctggagtcag
     5701 ctgagtagtt tgccgggaaa tattaactgc ctggaatttc atccttcacg gaaagaaatt
     5761 tattatgccg gaataggttc agtcttatat gtttctacta acggaggtat taatttttct
     5821 gtttataata atagcattcc caattttcat atctatggag tttttaaagc ccgaccagaa
     5881 ctcgattcta tttatgtagt tactgaaaga ggattatatt tagtttatga tcagtacatc
     5941 acaaatatca gacaaatttc cacagaagta cccgatcagt acagactttt cgacaatttt
     6001 ccaaacccat ttaacccaat gacaattata aaatatcaat gtacagtgta caatgatgtt
     6061 tcgctgaaag tctttgatgt attggggaat gaagtttcga ccctggtaaa tgaaaagcaa
     6121 actgcaggca gctactctgt ctcattcgac ggaagtaatt tcccaagcgg agtttatttt
     6181 tataaattgg aagcgggaga ttttgctgag acaaagcgga tggttttgat taaataaatt
     6241 ttattataaa tattataaga ttgtttttag ccgtaagatt agaagccgta tgctttagct
     6301 tgagagaatt cataaatatt tatttttcaa actaaaaatt tccaatcata ttctgaaact
     6361 gtttctatag atataaatga cagaagtaat tgcaataata catttgtaac attattttgt
     6421 atattaaaaa tatttcataa ccccctatat taaattttga ataaatttat tctattgttt
     6481 actctgcttg ctttttcagt ttcatccttt gcacaaaaat cagaagttcc aaagtatgga
     6541 ttttacggtg gacttggagg caactattct tttgtgaatt tcagccaggt ggatgctaat
     6601 acgctcggac tttctgaaat ttatataaat ggaaataaag ttcagaccgg cagcgcaaaa
     6661 ggtccttttt cgataacacc tgattccgaa agtaaattag gccctacact acaggttggt
     6721 tattttcaga agttcaataa tagcagctgg ctgtggggat caaaactctc atacagctat
     6781 gtgggtacta catcgacaag tcctgtcctt gagattcctc aattcggttc aaattccagc
     6841 ggcgatactt tcgaaggtgt cgctgtcgtt cagtcatttc aatacagcct taatcatcag
     6901 ttttcattta ctccttattt tggtatctcg tcgggtaaaa cattttttta tcttggagcg
     6961 ggaatttcaa tgtctgaaac caaagaagaa gttaacgatg tagtcgggta tgcttttatt
     7021 cagaatgaca ctgttaatat ctcaggtgaa cccacagatt tttcgagtac aaactgggtc
     7081 tggggttttg ctgcaactgc aggcggtacg tattttttaa gtcagtcatt gttcttagat
     7141 cttagctata cttttaattc aacccagaca caggttaatg attttaaagc accgttttca
     7201 aattcgaaag gaccattttc aacatcaggt gaactgatcg gaaggaccag cgctaatgaa
     7261 gtcaccaact ccattacact gactgttaat aagatctttt agaaatattt tttaagaaaa
     7321 gtttctatat ctgattttag tacaaagatt caaattcaga gagcatccat taaaactgat
     7381 tttcaatttt gacggatgct ttttatttat tagttttaaa aattgtaccg ggattaattg
     7441 gaattgtgaa cacatataaa gtcagatcca ttcaggatct ttctttgtca ttgcgttatt
     7501 cacatctcct gtattcagag tacctgcagg ctcaaacagc ataacctgta cttcctcatt
     7561 actatgcggt ctgtgctcta caccttttgg aatgacgatc atttcacctt cattaagttc
     7621 aagtgttttt tgcaaaactt cattcccctc ttttgttttg atatccatat aaaattttcc
     7681 tttgatcaca aggaacattt catcttcatt gtcatgctta tgccatacaa agtcaccaat
     7741 gaattttcct aactttacat gctgaccatt cagctcagca atgatctttg gactgaaatg
     7801 atcggagaac gctttaaact ttttgtccag gtttattaca ttcattatta ttttctaaat
     7861 taattatttt cagaaataaa caaatccata attactgctg ctaatagtat ttcaaaagtg
     7921 gattagtaat gcggagattt atctgcagat ctgttagtga atttaagcca gattctttca
     7981 tgaatataat acaacagcat tttagtaaag acttccgtaa atccaatttc caaagctctg
     8041 ttaatactgc cggtaacaaa atatgaaatg atcatggtat caatagtacc taaaattctc
     8101 cagctgatgc tttttgcaat actcttccag tgcttatctg tatatacttt taccccatta
     8161 ttttcaccta atactttatt accgatcttc agaaaattcc ataatctttc atgaaaataa
     8221 tacaatgcaa tttttgtgat cagctctata ccgccaatct taattgcaaa attaagattt
     8281 cctgtaaaga gccatgcaag gaaaattgta tccagcgttc cgactatccg ccatgatata
     8341 ccttttataa tacttatttt tctggaggat actgcgctca atagcttagt aaaatttgat
     8401 tacccgatct ttagacgttt ttaaaaaacc taatttaaaa tttttatttg actaaaatag
     8461 aaatttaatc tattaaatta agtatgattt gaaataattt tcttttttta atagtggtta
     8521 acatccattt gaataaagtt taatatgaaa aataattttt tgtttataaa tttaatgaaa
     8581 ccgcgatttg attaaatcac agggttttag gataaattta attttgatga taaatgatct
     8641 tctgttttta ataaaaaaat cccgcagaga aattatttat ctctgcgggt tagtaaaatt
     8701 aactgtctga caaattgttt aagtcagttg taatttattt caaaagaatc attttctttg
     8761 tctgaacaaa atcattaacc tgaattttat aaaaataaac tccgctgttt aatgaagcac
     8821 cgtcaaacct gacctcatag ttgccggaat tctgagacct gtttacaagt gaggcgactt
     8881 cttttccaat agagttatat attttcaatg agacatgacc ttttacagga attgaatatg
     8941 caatcttagt ttccgggtta aacgggttag gataattttg gctaagcaca aattcatcag
     9001 gtaccaaatt atttatatga gaaacaccga ctgtaccatc gacaacagca gtataacatt
     9061 ccatattaaa tccgccggtt ctttcatcag tccatacggg tactatcttt ccaccataat
     9121 aatctattcc tatataatca ccgaaccgga tatccgaatt tggctggatg gtaggcgaag
     9181 gaacatcact tagtggagtg tttgtaaatg aaagtcctcc atcagttgat aatgcaagat
     9241 atgcttcgac tatattattg gcagatgtat ttcttgtatc ataaaaaatg actgcgatat
     9301 ttcctgaatc actcactgca agccacggcc agcattgcat ttttccgttg cttacaggat
     9361 cgttatttac tctgagagga gaagaccagt tagcaccttt gttagttgat cttgtaagaa
     9421 aaatgtcagc atcaccattt ctgccgtcgc accatactaa atatatattc ccgttattcg
     9481 ctcctcctga aacatcagta tctatagaag gaaaagtgac accactggac gtaattggaa
     9541 ttgtaggagt aagcccctgc tgaataactc tgtccgttcc gaacgataat ccgccgtcag
     9601 tggacttgtc aaaataaata taatcctgtg tagctgtccc accgacccat gtgacataga
     9661 tctctccgtc aagccctatt gcaagatctg aaccctggcc cggacttgaa gggtcagcaa
     9721 ttgcaactgc attagaccag tttgatcctg agtttgtaga ccgtgtgagc ttgatacctg
     9781 agttgccgct gaatcttgtc cagctgatat aaagattatt ataatatgga ctccccggag
     9841 taaaatctgt ctcaagccat tctttgtctt ctgtaccatc attagctact atagtggata
     9901 tcgggaatgt aactccgcca tcagtggatt tataaattgc aagagcaaga ttctgtccga
     9961 cagtaattac agcaatgtaa aaatttcctg ctgtatcgac agcaactaca gggtcactgt
    10021 ttctcggaaa atcaggaaag actgttgaat caagtaactg acttaccgac cacgtaactc
    10081 caccgtcagt ggaataactg taaccgactc ttctatttgc cggactaacg ccttgtctga
    10141 aatctctcca tgctgctact actctgtttg gatctgtttt actgattttc acggatgttt
    10201 cattctgcgg aaagctgtct cttgaaatgt tagtatttgt aaagagaact gaattataat
    10261 tcgggatatg atcagaaaat gataaggtat tttttttctt gttccagaaa atttgctcta
    10321 cttcgtcatg aggttttgaa aatctttcag gtacctgtga ttgtacatta gccgaaagaa
    10381 agaacatcag aataaatata agaatgtaaa agtttttcat aatatcaaaa ttaaattatt
    10441 gaaattctat tttttcattt tcctaaaacc taatgcaatc aaacctatta caattccaaa
    10501 aattattcca ataaaaggtc ccatgaaaag tattacatac tgaggttcca tagacattgg
    10561 aatttcctta aaaccatcta aagatcctgg gttattttcc aataatttat caaaaaaaac
    10621 ggcttgaaaa acagagacaa taattcccat aaacaaacct aatacaactg attgggtcat
    10681 gaccagcttg gttgtgtgtt tggatattaa atatcctgaa ataacggcga taatcagcca
    10741 tgggaaaaat tcaaaacctg ttggtataaa tccgaaaact gaaagaactc ccatcagaag
    10801 tccaaacaaa gacaatttta aaattaaatt ccagttcatt agtaattttt tttttaattt
    10861 ataaatagtt tatttatatt actgtaattt tatcaatgaa tccagcgtat tctttgcttt
    10921 caaatcaccc ggattaattt caatgatcct gcttaaatta ctcatagctt tgctgacttc
    10981 attcttttta agatataagg taaccagacc gttcaatgca ttcacattat ttttatctat
    11041 ttttactgcc tcggaaaaat atttttcagt ctgatcatta tctttaagct ctgcataaca
    11101 cattgctata ttattataaa atcctgattt agaaggaagg tcatttgaaa gctcggctag
    11161 ttttttaaga tcctcaatag cttctttata ttttttttca ttaaaatatt gataagatct
    11221 cttctgcaga aaactaattt caaattttcc atcatctttc ttatcttttt tagaatcagg
    11281 attttttaag ttttcaagta aagagggtga atccatgtaa ttcggattta tggtctgaag
    11341 tttctcaagc aattgtattg cttcttcatt ttttccaaga gtattataaa ttgtagcaag
    11401 ctgataataa ccatcagctg catttgaatc aatcataatg cctctgctta tcctcatctc
    11461 tgctgaatca agttttcctt caatcatata aactaatccc agattatgat gtgcaagtaa
    11521 attattatct cttaattcta ttgcacgttt atatttcttt tttgcctcat caaatttttt
    11581 gcttttagaa tatatatttc cttcattgac tagaattacc gaaccttcat aaccaattgc
    11641 tgacgaaaat aatgtttcat ttgttttcca gtccatgttc cttagatatg tcatatatcc
    11701 aagagaaatt attattatta aaacacctac aattaacatt ccaaaatccc ttttattact
    11761 tgcaagcatt agaaggtgtg taattagaac tataagtgaa aatgaagtca gatacaaaaa
    11821 cctttctgcc ataaggttca tagtcggaat aatattcata acaggaagaa gtgtcacaag
    11881 aaaaaaaagt atgcaaaaag atattatgct gtcccgcttg tagaagaaaa cagaaagtcc
    11941 aattaatgca aaaataaaga tcagtgacaa aagggcacta aaatcaagta tggatgaaga
    12001 gtctgtgata acgccattat aatgataaag taatccaaac ggtgcaaaca ataatctgaa
    12061 ataaacagga attgttttta tcatagtcca gaaaaccact tctgtatctt tgttaaagaa
    12121 atagagatag ctgtcacgat caggtacacc tgacaatacg ctttctctta tcaataaata
    12181 aattcctgtg actgcaataa acaataaata ggaaagataa ttttctttca gatactgaag
    12241 attttttttt ctgtaaacaa aatcataaag taaaattatt acgggcattg ttacgatcat
    12301 ctcttttgac agaagtccaa atacataaaa aagaagagat aaaatcagat acagataatt
    12361 tccggaattg tcttgatcag atcttttatc catggttcca tgatgactgt aaccggtaaa
    12421 caatatataa aaaagaaatg aggcaaagaa gaacattgtt acaatagaat ctgtcctgcc
    12481 gcatatccat gtaactgctt ctgtatgaat gggatgaact gcaaagatca gcgagccgaa
    12541 aagggagaat aaatttctgt atttatatct tgagaacatt aacgataaaa tcctgaataa
    12601 caaaagacag gaaattatat gtattataat attagtgaga tgaaatccat aaggatccat
    12661 tccccagatg ctgaaatcca ttaaatatga agaagacaca ataggtctgt aatatttacc
    12721 aatgacttta tgaaagcctt cttcacctgt aaaatatttt aaaacgttat cggcattttg
    12781 aatggaagga ttatttacta ctacactttc atcatcaaat acaaacccat tttgaagtga
    12841 gttgttaaaa acaaagtaag taactgcaat aattatcaga tagcaataaa taggtttgat
    12901 cttactcatt tatatgacta tgattaatgt tcaatgagtt gaaaataata atttataata
    12961 actattatta gtaaaatata agtatatgaa aaaattaatg gttctataat taaaatgtat
    13021 tattctgaat aacctgcgaa aactttcaga caaaggcttc gacactttca ttggacatta
    13081 atcttttagg attatctctt ggtactgttc ttctcataaa atcaaaaaat aatttagtcg
    13141 tagtacagga aggacaaata ccaatatctc cgtcccatat ttcatcacaa aatgcatcgg
    13201 gcagattttt atgaattatt tccgtagctt tttcatgaac atcccagacc cagtcagtgt
    13261 caggtgcttc tttcccttca taatcagttc cgaccatagg gacgaacgga ctcatgatag
    13321 gaaatattcc ttctgatgac agataatcta taccttttaa aactgattcc attggttcaa
    13381 gtcctactac gaatgagcta agcgcacgtg caggtttcaa aagatctctt gcttctttga
    13441 gagattctat ccatctttcc ctcccaactc ttctagtctt tcccggacag atctcattaa
    13501 aaatttgctc atcccagatc tcaagattga atgcaactga actaattcct gtggaacaaa
    13561 gatactgagt ctcttcaata tttcttgtag cagtaattgc tgttacaatg ggaaaatatt
    13621 tttcagataa aagcggctta agtctttcta gaatctttgg aagattccca agtccgtcat
    13681 cttttgctgc acctgcagtc attgctaaat gtgaacagac aggattatcg attattgctt
    13741 ctttgactac atcataaatg tcttctgctt cttttattgt aacgacgtga tctctgtttc
    13801 tttgtcgtgc aggaacaatg ttacaaaatt tacattgctc acctttggca aaataagtac
    13861 aggcattatc aaaacatact ctcaatccac agtcgccacg catagaagct actttcttca
    13921 tagattcccc gctgcgtgtt ttcatatcat aaaaatcagg tctctttggc caccggactt
    13981 cacaaagttc tttaccattc tttaaaagaa ttactgcatc accacttttg tgtaccctaa
    14041 acggtgaatc cggactgtat cttacatctg cataaagatc atgcggaagc agcatatcat
    14101 ttggcaaagg tcttgtaccg tcacctgtac ccttatgagt agatggtgtc atttcaaata
    14161 cccagaaaac tttttcaagt ccaccttgtc caacagttaa taatgcttca tcagaaaatg
    14221 taagtccttt gataagtaat tctgttttta atctgatggc ttcatctaaa gtcatatttt
    14281 actttgttga atcttaataa attctaatta aaaaattgga tcaggtattt gattctggaa
    14341 cattgatatt taaaatttcc tttgaaagct cacagcatga atgaggttca ttgattcccg
    14401 aattctcttt tataatgtca aacaaaaatc cacctgtaaa acattcagcg caaattggag
    14461 catcatattt ccagtaatct gaagtggaaa taaacggcag tttctctgac attatttctg
    14521 cagcttggta atgagtatca aacatccatt tataagtcgg cgatctgaac ccttcaaact
    14581 gacttcccgt ttctggactg aatggaacga tgatcggtac aattcccctg tcacagcacc
    14641 agtgaacacc ttcaagtaaa gtcttggcag gttcaagtcc ggcaatcatt gcagtaaata
    14701 ctttaccctc tccaaaatgc tttacagcag ttataagtga ttcttcccat agctctcttc
    14761 cgatattctc atcttttccg ggtacaattg ccttccagta tcctttgtca aaaacttcaa
    14821 aattataaga cacagaatgc agtccaagat catataataa ttttacatca tcaatatttt
    14881 ttgtcggaga aattgtcatg agcagctttg ttctttctct gtcattttta tagggaaatc
    14941 tttttgataa ttcttcaaga atctgtacag ttcctccaag tccctgttta tcgggaggag
    15001 ttcctcttgt taaaattgat ctcggatctg tatgatattt ttttgctttt tcgattacgt
    15061 cagcaacctg tgaagcagtt tttcttgcgt tagaaatatt ttcatctgga taaaaatcct
    15121 gagctggtgt aagattacaa aatctgcact cagtaccggt cctgaaatag tcgcaataat
    15181 aattccaggt aaaattcaga ttacacattc cgttatagac tacaactttt tcaaatggtg
    15241 taagatcatt tgtatagctt tttgagaatg gaggcagtga cacaaactca ccgcttgtaa
    15301 ttacatttcc aactttatct tctattgtta gatcactctc tgatattctg atcctgaaat
    15361 ttgcttcagg attttctctt acctgcacat aaacattgcc gtccagtttt atttcagcag
    15421 gaagaaattt tacagctctg tctctatccc atccctgtgt ttcgaccagg tcacttctaa
    15481 gttcatttaa tctgacccca tttataagca gatcacattt taattttatt gttttttttc
    15541 tatctgtcat tcttaaaaaa aatttaaaaa tatttaaaaa aaagtcccgt caattttcat
    15601 gaacgggact ttaataaaaa actaagttac atcattattt ccaggatccg gcgggtggaa
    15661 aaattgagtg cgacttttca gaagcattat cccatgaacc tgccggtgga aaaattgaat
    15721 gagacttttc tgcagcacaa tcccacgacc ctgcaggtgg gaaaatcgaa tgtgatttct
    15781 cacttgcatt atcccaagat ccagccggtg gaaaaatcga atgtgacttt tcacttgccg
    15841 aattccatga acctgctggc gggaatattg aatgagattt ctctcctgca ttattccatg
    15901 aacctgccgg tgggaatatt gtgtgtgaag tttgctttac ttcgctcata aataatttta
    15961 gtttgtaaat aatatatcat taacgggtac aacttaacta atttcaaacg ctttttcaat
    16021 taacatattc agtaacaaca attattcttt accgaaaaac attgattaaa atattcggga
    16081 agatcttcag atcaatttta cagatcaaat aaaaacacct tctactttaa tttcaagcag
    16141 aaggtgaaat aatataaaaa tactgaataa atatctgtta tgaatttttt actgttaaga
    16201 ttttttaatc agttttattg cttttacaaa tcaataaccg ttaagtttat tttaaagctt
    16261 tcttaacagc tttttttact gcagcatttt taacagcttt tttaactact gcttttttta
    16321 ctgctgcttt tttaacagca tttttaacta ctgctttttt aacagctttt tttactactg
    16381 cttttttaac agcttttttt actgcagctt ttttaacagc attttttact accgcttttt
    16441 ttactgctgc tttttttact gctgcttttt tagttggtaa aactttttta gccattttat
    16501 ttttttttat ttagttaaaa aaattcttac aaatataaag ttattaactt aaaacatcaa
    16561 ttatattaat ttgcatgaag attttttttt acgatcatca caaaattcag attgtgattt
    16621 taacaaaaaa atattgattt aatttatttt caaaaattaa ttttgaattt tattaaaaaa
    16681 taaaaatgag actttcctgc aaacatctgt aaacaaaggg tctgcgcgtc gttaataata
    16741 ttttttcaat gtgttttaaa aataaactgc aacagatctg aaatataatt ttaatttatt
    16801 ttcagcaaat aaaatatttc ctcataaaag aaaaatattt attttgaaaa tttaatctta
    16861 attttttcaa caataaaatt ttttggaaaa attcagacta aataaaaaaa atattttcga
    16921 aaaataattc ttaattttat tcaacagtaa aaatctttta acaaaaagtg actaagaaaa
    16981 aactatttta cagaatattc atgttcaact ttcaaagaaa ttaggaaatt gaatttaaca
    17041 attgattaaa ttaatttata aaaaattatt cagtactaaa aacatttaat ttattttaat
    17101 taaaatttat taacacctaa aataaattct tgtgaacttt attttcaatt tcttcattcg
    17161 aactaaatac tttaacagaa atatttttat tatttatata taaaggaaat ccttaatgag
    17221 aaaattattt atattaattc ctgtccttat tatagtattt tttataatta ccggacaaaa
    17281 aaatcccgag tcagagaaat ggagcaataa tttttcaaca aatctatatg atccgggagt
    17341 aataaattct gcagtacttc cacaggtcta tgatttcaca aatccaaata cagagacccg
    17401 tcatgtaaag actccttatg aggtaatttc agtttcacct aatataagag tattgccgag
    17461 gactaacagt tatcaaagtg aagttgatat agtcaggcat cctacaaatc caatgatatt
    17521 gtttggatct tcaaatgctt ttaataatac aggtactctt tttattagtg aaggtgtcta
    17581 tgtatctact gatggcggca ttacatgggg cggctctgat acaatgaaat tagccggagg
    17641 agcaccggtt ccaaatcagg gcggagaccc cggtatcaca atagataaga acggaacatt
    17701 tattcagaca catcttggat tcagtacttc ggggatgttt ggaaattatt ctacagataa
    17761 cggatcgaca tggtcatcaa attatacaat agcttccgga tctcaggata aaaatcttgc
    17821 agggacagat gactccccca ccagtcctta ttatggaaga tcatattgtg tctggtcatt
    17881 gtttacatca ggatcaccgc caattgatat ttcatatact acaaacggcg gagtttcatg
    17941 gtcaactcct cagcagataa acactcctcc ttcaggacat tattcacagg gatgtgatat
    18001 aagaacaggt ccaaacggag aagtttatgt tatctgggct gcaccggttg ccggggttcc
    18061 atttacagaa gactttgcag gatttggaag atctactgac ggcggcgtaa actggacagt
    18121 aactgaaaat gcatacgaca tgaatggtat cagaggaatt ttctcatcaa agtccagtat
    18181 aagagttaat ggttttccaa gaattgatgt tgacagaagc ggtggtgtca ggaacggctg
    18241 gatatatact gttgcttcag agaaaaatat ttctcccgca ggttctgatc ctgatattat
    18301 tatgcacaga tcttcaaacg gcggcacgac atggtctccg ggtatcagag taaatcagga
    18361 tccaattaat aacggaaagt ttcagtggtt cccggcaatt cgagtcgatg aagcaggtgg
    18421 agtgaacata gtttattatg atgacagata tactcaatca gactcatcag aagtttatat
    18481 ttcaagatca ttagatggag gaaattcatg gtcggatatt caggtaagtg atcacagatt
    18541 taaaccacag tctatcgggg ttgcaggaat tgctacagga tatcagggag attatattgg
    18601 gatgacttca agtaatggta atctttttcc tatttggatg gataatttct cagggatata
    18661 taatgcctgg actgcaccgg ttctgatctc aacttatcct ttaaattcat ttaatctaac
    18721 tgctcctccg gcaggaacaa ggatttccac tttacaaaac agtacaacta attttacttt
    18781 tgactgggat acatcttctt caacttcatc ctataaatgg atttttggaa gtccgactac
    18841 aaatccaaga ttactttcag ttaattctaa tacaaatact ttatcaatga ctcttggtca
    18901 gcttgataat atgctttcat cacttggtct aaatcaaggc gactcacttg tcggtcagtg
    18961 ggatgtgtgg gcatttagaa ataatcagac agatgattca ttgaaagcag caaacggatc
    19021 aaggacgatt actttgaaaa gaggagtacc ggtacttagt ccttttagtt taatttctcc
    19081 tgtgaataat acaactattc tttctttaaa tacaaattct gacactgtaa atttcagctg
    19141 gtcaaattcc ggacaggcag taaattataa atggctttat gcatctccgg attttagtaa
    19201 tctgtcaaat ataaaattaa gaattaatgc tgacaacaac agctttgatc cttcacttac
    19261 attaagaaat tcacagatcg actcagctct ggcaggaatt ggtataaatg taggcgattc
    19321 atcaaccggg cagtggagag tttatggata tagtgcaggt gattcactcg cttcgacaca
    19381 gacttataat attaatttac gaagaggatt tccgccgact gtttcttcat ctgcagattc
    19441 gattattgtg aatgtattat taggacaaaa cttttctaca aatcttatca ttggaaatac
    19501 cgggcaattt tcgcttgact ggtttatttc agaatctcct gcagcactgg ataatctttt
    19561 tatcagatcc gaatttaaaa ttgataatga actaaacctt cagcagtcaa acctgactaa
    19621 aggatctgcg gatatttatc atggtcagga tgtgacagac ggaatgggcg gtccggataa
    19681 ttttggttat agatggatag acagtgatga gccgggcgga cctgtctttg actggtttga
    19741 aatttctaca ataggaactc agatcacgaa ctggacaagt ggtactgctg atgacggatc
    19801 agtgatcatt ccgattccat ttaattttaa tttttacggt accgactata cacagctaaa
    19861 gatctgtacg aacggctgga tgggatttga cattgcaagc acttcaaatg aatacaataa
    19921 tattgcgata ccatctacta tagaacctga taatgctctt tttccttttt gggatgatca
    19981 tgatctgaga aacggaggtc aggtatttta ttttaatgat gcatccagta acagatttat
    20041 aattgaatat aaagatgcac ctcatttctc aacaggaggt ccatatactt atcaggtaat
    20101 gctgtactca gacggtagaa tttattttca atatcttact atgaacgatc ctgtaaattc
    20161 ggcaactgtc ggtatagaaa attctacagg tacagatgct ttacaggttg cattcaatac
    20221 accgtatatt catgataatc ttgccataaa aattgaaaaa ggattacact gggtcacgga
    20281 gaatccagtt acaggtatta tagatccggg tggaaatcaa aatgtaaata ttgattttaa
    20341 ttcttctact ttatctcagg gaatatataa tggtttcctg caaatagaaa gtaatgatcc
    20401 tgcgactcct gtcaagaatg taaaattaaa atttaatgtc ggaacggtag gcattcagaa
    20461 tgttcagaca gggattccgt cacaatattt acttgcgcag aattatccaa atccgtttaa
    20521 tcctgttacc aatataaatt tctcattacc taaagatgga ttagttactt tgaaagttta
    20581 tgatatttta ggaaaggaag taattacact tgtaaatgag aataagcagg caggtaatta
    20641 cagagttgat ttcaacggat caaactttgc aagcggaatg tatttttata aaatagagtc
    20701 cggaagtttt gttcagacga gacgtatgat acttctaaaa taattaaata ataaaaattc
    20761 ggaattaaga attttaaaga ttaaaattta tcccgcagtt attaaatttt ctctgcggga
    20821 tttttttttg tatttgcaaa cccgaattca tttgagtgca gcaatcccaa aaatgaaatg
    20881 tctttgcgag cctgtgatat tcaggcgaag caatctccga atttacggtt tgtgatgctt
    20941 agaaattctt gtcatggatt cggggattgc ttcgcttcgc ccgcaaaatc atatcgttat
    21001 cttgagaagg aaaattctct gcgg
//
LOCUS       DAORVQ010000034        21013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_975_length_21013_cov_6.753078, whole genome shotgun sequence.
ACCESSION   DAORVQ010000034 DAORVQ010000000
VERSION     DAORVQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 21013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21013
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_21013_cov_6.753078"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..796)
                     /locus_tag="PLX80_04635"
     CDS             complement(260..796)
                     /locus_tag="PLX80_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQY20116.1"
                     /translation="MDKENLGRHYLENVRSEFRKLKSLGDNTFKQIKDEEFFLSPDEE
                     SNSIAIIVRHMSGNMLSRWTDFLTTDGEKEFRKRDEEFDKLFYTDKDDIILRWENGWE
                     CFFKTLDSLTEDDLLKYIKIRNESHTVTEALNRQLTHYAYHIGQIVYLAKHILNKNWN
                     SLSIPKGKSDEFNKKMGM"
     gene            complement(812..2218)
                     /locus_tag="PLX80_04640"
     CDS             complement(812..2218)
                     /locus_tag="PLX80_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQY20117.1"
                     /translation="MTFGSSNDICAQEKIELKNAKELTGSTVNGENVREANGDVEFVQ
                     GNVRVFCNSATQFITSNRVELRGDVKIYQDTLTLLTNKATYFGNDKRAICEGGVTLKD
                     PNATIRADNGIYTFNESKAVFTGDVIIINPEYRITSSTLTYLRNTEDSFAEGNVIVTT
                     DSAVIKADKIDFYKSAGRTFARGNVFIESDSTVITSDTATNYSVEKKSVASGNVKIES
                     LNNSSVIYGDYIENFENENFTILKKNARLIQIEESEIADTLFIYSDTMKAYRNKPDYY
                     IAEGNVEMIRNDFLAKCGYGIYYRELETVSLSEKPVVWQDRLQLTGDSIYAELPGKKI
                     SNIYSKKLNDKPGTESSFALTSNEDEYFSDRYDQIRGDDIIIKFSDDKINEIEVDNNS
                     SSVYFVYEENKANGANKIEGSRMYLYFDDEEKISKIKIETEPKGEYIPESLLNTVSLI
                     LPGFEVITDRPVRRSVNN"
     gene            complement(2741..3274)
                     /gene="lptC"
                     /locus_tag="PLX80_04645"
     CDS             complement(2741..3274)
                     /gene="lptC"
                     /locus_tag="PLX80_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQY20118.1"
                     /translation="MLKSLTVLFFLTLTIFIYSCDDNFKPATINSESNDMPTQESWNS
                     TVVFSDSGNIKAILTAGHIAMYTTKGFTLIDSGAKVEFYRDSVIVSVLTGKKGKIDDK
                     TKDIEISDSVTVVNTEGSQLKTEKLIWKNAEQKVYSDEFVRITTPDETIEGTGFESDQ
                     NLKNYKIYKVSGVFNGN"
     gene            complement(3276..4268)
                     /locus_tag="PLX80_04650"
     CDS             complement(3276..4268)
                     /locus_tag="PLX80_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQY20119.1"
                     /translation="MQKTSTVKNIKNGRNVVSIEKKSIDDLHKRFSSKNFSGKFSEAV
                     ELIYRCKGKVVVTGIGKSGIIAYKIAATFNSTGTYAFFLNSADSIHGDMGVIRKGDVA
                     LIISKSGDITEIRHLISALKSMSVKIISIVGNLESELAKASEIVLDASVKKEACPHNL
                     APTSSTTVSLVLGDAIAIALLEKNEFTSDNFALVHPGGNLGKRLLLKVGDIMSKDIDV
                     PVVQVNSKLKDVIYMISSKRLGCACVTDRSKIAGIITDGDIRRLLEKNLDGLNEITAA
                     EIMNSNPKIISKDMMAISALEIMEKNKIMQLIVGDKSKKPIGMIHMHRLIEEGL"
     gene            4355..5791
                     /gene="rlmD"
                     /locus_tag="PLX80_04655"
     CDS             4355..5791
                     /gene="rlmD"
                     /locus_tag="PLX80_04655"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQY20120.1"
                     /translation="MVKKGDEIEVKILELNSEGKGVSKIEGGFVIFTDKALPGDEALV
                     RIRKKKSKYAEAELVNLITPSAFRIEPVCNYFGTCGGCKVMNYEYGKQLDFKTNAVKN
                     AFERIGRFTDLTIPQAIGSKEIYFFRNKMDFSFSDDEWVENYERPERNSDENYVRQPG
                     FALGLHVPGFHSKIISIDKCYLHSDLSNEILKFTSDFFKKRNTTIYSTKTHEGYLRFL
                     VLRDGKNTGEFMLNLMTNSYDKELFAEYSDKINELFPVITTIINSVSRKKAQIAVADE
                     EYIVKGNGYIYEKLKRGKDGSELKFRISAGSFFQTNSHQAEVLFDETVRMSDLKKTDN
                     VLDLYCGGGSISLFISEYVNKVTGVELIQSAIDDAKINMELNGIQNTEFLVSDIKDFL
                     TDIKNIGEYNKVILDPPRSGLHPKICEILSATDFEKIIYVSCNPHTQARDLQLICAGG
                     KYIIEKIQPVDMFPHTYHIENIVTLVRA"
     gene            6218..7810
                     /locus_tag="PLX80_04660"
     CDS             6218..7810
                     /locus_tag="PLX80_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HQY20121.1"
                     /translation="MQTTDMQTANKDKNNILWVDDEIESLKSNIMFLKQKGYNVHEAT
                     NGEDGVNLIKKNDFDLVFLDEMMTGMGGLQTLIEIKDLKPSLPVVMVTKNETESLMED
                     AIGKKISDYLIKPVNPTQLLLVCKKMLESKRLKGDQVSRDYIQEFNKLTMAMSDNPGW
                     EEWIDIYLKLTGFDMELDHHPELGLRQTIFDQKRDCNIEFSKFVEKNYLGWVNSTSDK
                     PILSNEIVDKFIIPELDTNESVFLFVIDCMRLDQWLEMEKYLYDYFNIKKSYHYSILP
                     TATPYSRNAIFAGLFPSEIEHHYPELWKKNDDDENSKNNYEREFLIKLLERRRIKLRN
                     EVKYTKIMDSNFSRGIENKIMTYCQNHLNAIVINFVDMIAHSRSDNAILKEIAPDEAA
                     YRSLTASWFEHSSFFGMLRQLSTKKNIKIIITTDHGSIRCLHGVKAMGDKDTSTNLRY
                     KCGRNVKADARNAIMIKNPLEYKLPKRNGIINYIIAKEDFYFVYPTDYHKYLNQYNDT
                     FQHGGISIDEMILPVITLDSKV"
     gene            7810..8247
                     /gene="tsaE"
                     /locus_tag="PLX80_04665"
     CDS             7810..8247
                     /gene="tsaE"
                     /locus_tag="PLX80_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQY20122.1"
                     /translation="MERIVTSVSTDYTEEAGRVYASTLDPGTITGLFGNLGSGKTQFV
                     KGVCRYFNVKEIVNSPTFIIKNEYKGKDPVSENVINIHHFDLYRINNINELTQLGLNV
                     IDNESTILLIEWAELAEEYFRGNISKIFFDYGQKENERIIKFA"
     gene            8252..8899
                     /gene="tsaB"
                     /locus_tag="PLX80_04670"
     CDS             8252..8899
                     /gene="tsaB"
                     /locus_tag="PLX80_04670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQY20123.1"
                     /translation="MNILFLDTSSKISETAFYENEKMIFHLILKESDGADQVVYNLRQ
                     SFKEFKLDPNNIECLSLSNGPGSFTGLRIGSAVAKGICFVSGCKFIEIPTLDILANII
                     PSGNSITSLIPSNPKTSEFYFAKYEKSGNDLKRTSDFDFGNLHEILKTDSQFVIKKEA
                     FQYIPEPIQSKFFEYPDDAVMRSQYEITLKKISDNEFSDVENSTPIYIRQFIPKN"
     gene            8946..9788
                     /gene="accD"
                     /locus_tag="PLX80_04675"
     CDS             8946..9788
                     /gene="accD"
                     /locus_tag="PLX80_04675"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQY20124.1"
                     /translation="MAWFKRSKENISTLEPKKEVSDGNWLKCSECSEMIHKKTLESNF
                     YICTHCGNHFRIGSSEYFNILLDEGSFKEMDKKVKSVDPLNFEDTKPYKKRIDDTIKN
                     TELYDAIRTGTGTINSIDVVIGCMDFNFIGGSMGSVVGEKISRAIDKSIKTKYPLIII
                     SSSGGARMMEGAISLMQMAKTSTKLAKLSEEKIPYISIMTNPTTGGSTASFSMLGDLN
                     IAEPKALIAFAGPRIVKQATGKDLPPGFQKSEFLLEHGFIDLIVNRKDLKEKLTQIFN
                     LLQN"
     gene            9788..10648
                     /gene="panC"
                     /locus_tag="PLX80_04680"
     CDS             9788..10648
                     /gene="panC"
                     /locus_tag="PLX80_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQY20125.1"
                     /translation="MKVIKEVRAVQQISGFYRCTGKKVGLVPTMGFLHEGHTSLMLKA
                     KDECDILIVSIFVNPTQFGKGEDFDKYPRDIVRDFHICEKSGADYIFNPEVSEMYGEN
                     NFTKVSLSNVTEKLEGDIRPGHFDGVATVVTKLLNISKPHRIYLGQKDAQQNAVLKRM
                     ISDLNIDTDLIVCETIREENGLAKSSRNTFLTNEQKEKASVLYFILNEGKRLIMEEKA
                     NDSGIVYEFVKDILKEKTPEFELQYYKITDNILLEEIKDPSSFKGEILISLACKAGNT
                     RLIDNIIFNK"
     gene            10691..11440
                     /locus_tag="PLX80_04685"
     CDS             10691..11440
                     /locus_tag="PLX80_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQY20126.1"
                     /translation="MNRNLATVIMAAGKGKRMNNPDKSKVMFELNGKPMIEYVVSLAE
                     QINSEKIIPIVGHQKEFVMNFLNDKFSNISDKLSYAHQDVQLGTGHALMQTREHLSNF
                     EGDVLILSGDVPLLTEKTLRSFIKFHEENNFEASLISAIPDDPTGYGRVLRNEENEFY
                     DIREHKDCNEEELKCREINSGIYIIDSKILFEALKTIKTDNAQGEYYLTDIFKYFRSK
                     GMKTGAFVTDNVIEITGVNSTEQLKDLESNI"
     gene            11477..12871
                     /gene="glmM"
                     /locus_tag="PLX80_04690"
     CDS             11477..12871
                     /gene="glmM"
                     /locus_tag="PLX80_04690"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQY20127.1"
                     /translation="MSELIVSVSGIRGITGDSLTPQNIIKYTSAFAEYCRKNSKKKKF
                     RIAVGRDGRLNGDITEGIVINTLRMCGADVISIGIAPTPTVQIATEDMKCAGGISITA
                     SHNPQIWNGLKFLNPDGTFLDGDQIEDFKKIAKSEKFEYADLLHTGKIISDNSMSDHH
                     ISKVLKLKILDLKKIRSRKFKVVVDAVNSSGSVIVPTLLKELGCKVMELHCDRSGIFP
                     HIPEPLPQNLKSLSKAVKKFKADIGISVDPDADRLVMIMENGKPFGEENTITMIVNHV
                     LRNQSGKNNSAVVNLSTTRAVEDLAAVHNSKVFRSPVGEINVVKEMMKRKSVIGGEGS
                     GGVIYPELHYGRDSLVGIALALNELAQSGLSLSEYLNRLPAYTISKAKIENVKDPENI
                     LKQLEKKFRRETNITGIRTDDGLKIDFNDHWVHLRKSNTEPIIRIITEAAKGLDADKI
                     NKNFIDYIIKLNKN"
     gene            12949..13602
                     /locus_tag="PLX80_04695"
     CDS             12949..13602
                     /locus_tag="PLX80_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20128.1"
                     /translation="MKKILLTVIVLFTAFTLYSCSSVTEMSGTWKKPGTTATKYNKIV
                     VMGVSKNLVTKSTVENAIVSQLKKNGFNAVAGANVLPTSTFDADADGKADDDSKSKIA
                     EKLKELGVDGALVLSLLDKKESERYVPGTTMYSPYNSYYPFYGYYGGAYNMVNTPGYY
                     EKVTNYFLTSNFYNVGTEQLVWSGQSETFNPTSLSDFSNSYAEKVVDELLSSGIVRK"
     gene            complement(13617..13748)
                     /locus_tag="PLX80_04700"
     CDS             complement(13617..13748)
                     /locus_tag="PLX80_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20129.1"
                     /translation="MNRNKLKILEGSYNGLYNDFRFFAGRLKIKKGKTQKRFSPKEI"
     gene            13794..15218
                     /locus_tag="PLX80_04705"
     CDS             13794..15218
                     /locus_tag="PLX80_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQY20130.1"
                     /translation="MKTKISFLLNDTLIETGLNPATVLLDFIRSNDFTSAKEGCKEGD
                     CGACTVICGKLNNGKIIYRTINSCLVPVMNINGAHIVTTEGLNNVSGILTPVQKLFLK
                     EGASQCGFCTPGFIVSLTGFFLSDNFNDDILDSIDGNICRCTGHNSIIRAAEYFSSEL
                     KDIPEDFDCRIKYLIEKNIIPEFFLSAKEKLIYIQKNIEPKNNKNVSPKYFISGGTDL
                     FVQRPDEMLSSDVSFLSKENSQELIYEKDGSCFIDSTSTVTDIFESKLIQKLFPIISE
                     FTKLFGSTPIRNSATIGGNINNASPIGDMTAFFMALDADMILSDGNESREVSLKDFYT
                     GYKKTVRMPDEFVKSVKFKIPDGQFYFNFEKVSKRTYMDIASVNTALFLQMNEDVITK
                     INISAGGVFETPKFLSMTSDFLTGKKINTDVILEVLEITDSEISPISDARGSEKYKRL
                     LLKRLIWTHFIKLFPELINIKETV"
     gene            15215..17518
                     /locus_tag="PLX80_04710"
     CDS             15215..17518
                     /locus_tag="PLX80_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY20131.1"
                     /translation="MKNYDIIQHVTGKSQFIDDINVPNGTLFAKVFYSKNAHAKITSM
                     DLSEAESIKGVVRILTYKDIPGVNQVGGIVLDEPLLAEDEVHFIGMPVALVIAESMLI
                     AREASDKIKIEYKSLHVITDPREAFSKGLLSIPPRIFESGNTYEAWKECEFFETGTCE
                     TGGQEHLYLETHGAISIPMEGGKVKIISSTQSPTAVQRVTSGILGIPKNMIEVEVLRL
                     GGGFGGKEDQASHWAALSALAACILNRPVKLVLSRHDDMVMTGKRNPFSSDFKIGLNR
                     EGKILAYEVTFYQNSGAAADLSPAILDRALFHCTNSYYVPNVKATGYCCKTNLTPNTA
                     FRGFGGPQGKFVMESAIYKAAEKMDMDPFEIQRINLLKENDEFHYGQKALHVMAEKTW
                     DQCDKEFNIDTVKKKTEEFNSENNLYKKGFAVIPICFGIAFTNTRMNQANALVNIYTD
                     GSVGFSTAAVEMGQGVNAKIREVISRVFSISHDRIRSEPTNTTRSANTSPTAASSGAD
                     LNCNAVYLACNKILERLVQSAAEDLGEKNSGNIILKDEKFYLNGEETDLNWNNVIETA
                     YSKRISLCDYAHYSTPELCFDNKINKGNPFRYHVYGTAIIEVTVDCLRGIYKIDSVKA
                     VHDFGSSLNPVIDLGQAEGALIQGLGWMTIEEVIHDKKGRLLTDLLSTYKVPDVHFAP
                     KNIVIKFLNEPNPVGIFNSKAIGEPPLMYGIGVYFALLNAVREFNPDADLEFNAPFTP
                     EKVLLKLYEKKNMTEPKPVAVNHSESI"
     gene            17552..18535
                     /locus_tag="PLX80_04715"
     CDS             17552..18535
                     /locus_tag="PLX80_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQY20132.1"
                     /translation="MKDLSLWKFIQSELIKNNRVILFTVVHHEKGSPGKTGFKMAVSS
                     EGKFSGSIGGGVMEYNLLKSCDKLFLSGKKINTAEKYFHYKGKLPGKNILSEKASGLI
                     CSGSQIIAANLFTHKDLTMVKEIRSAYRLGKNVILNLDSTGLTVRPIEISEKRRFEFT
                     ASVKGLWSYEENLSLTDTILIAGSGHVGIALRKVMSELDFRILIYDDRKTPLFKFKKF
                     TGCRLIVDDYSHILTHLRNIKINYAVIVTTGFESDKVVLKNLINMNLKYLGLMGTKAK
                     IKKIFNEAAEEGISKVLLEKVYAPVGIDINSNTPEEIAISIAAEIIKIRNG"
     gene            complement(18750..19619)
                     /locus_tag="PLX80_04720"
     CDS             complement(18750..19619)
                     /locus_tag="PLX80_04720"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQY20133.1"
                     /translation="MEKDSLLNEPDKKKILRLIGSKDFDKIIGMLEKISSDHAGTAKT
                     KDKRFVIREIVREIIDKEGNRESEFYKTGMYFCKRKEDNAKEIGVSLIWRGYDHAPEK
                     VKEMLHRISDDPNWEVREYAGNAFAETLYYHKDFLTDLKEWSKHPSENVRRAVVFSAL
                     GVRDEKKYKTGFKILRSLMNDSSVYVKKNLGPFILGSLYGSRFPEETAAFLKKESRSK
                     DPYVRWNIIMSFNNSFGKRNPDKALEVMKVFTDDENIIVIRAMRSTLKFLKKHYPEKA
                     DKFIKENYELKFK"
     gene            complement(19812..>21013)
                     /locus_tag="PLX80_04725"
     CDS             complement(19812..>21013)
                     /locus_tag="PLX80_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20134.1"
                     /translation="SPRNCNNNVNGYNLIHGNRHFAFDLINSEGRYNWALDRTVSDPY
                     LGSFGIQKNLNGNRDSGVYEFNKYYLQTYPTWSAQLITDDFCSSASNDYFITGDFHGD
                     GLDGYNIGYDEIFSPYSNPTTRSCQDLSNNGLTIVLQSQDSYGAITLKIYYNDEDALI
                     DLPPSKPKNLKVGKQYFGEPESYKFYPVVTWDSNIEPDFYDAPAVSPLLITPVYEIYR
                     ADTTVCDIEPVYSLITTVSSTTTQYVDSNVTLYDNIPAIPDYCEYTLLTYSYKILAKD
                     NRGNRSLKSERGLLTGYNQECGPETEDLIESENNILPDMFSITNYPNPFNPTTNIKFD
                     LPKDVLVSIKIFDMLGREIRTLVNDYKTAGSYTISFNGSDLSSGIYYFKIKAEDFEQV
                     RKMMLLK"
BASE COUNT         7129 a         3540 c         3700 g         6644 t
ORIGIN      
        1 ctaaatctat acaaacattt tgaatcgtag agacgccatg catggcgtct ctacaatacg
       61 ttcatacgtc gcgttcatgc attacgttca tacgtcgcgt ccataacgtt aaatcatttt
      121 ctaaatctat acaaacattt ttgatccgta gagacgccat gcatggcgtc tctacattac
      181 gttcattcgt cgcgttcata cgttattaat tcaaacaaac atttttgatt cgtagagacg
      241 ccatgcatgg cgtctctact cacatcccca tctttttatt aaattcatct gatttccctt
      301 tgggaattga aagagaattc cagtttttat tcagaatatg ttttgctaaa taaactatct
      361 gtccgatatg ataagcataa tgtgtaagct gtcggttcaa tgcttctgtt actgtgtgag
      421 attcatttct gatctttata tattttaaaa gatcatcttc cgtaagagag tccagagttt
      481 taaagaaaca ttcccatccg ttttcccatc tcaaaataat atcatccttg tctgtataaa
      541 ataatttatc gaactcttcg tctctttttc tgaattcttt ttcaccgtca gtggtcagaa
      601 aatccgtcca cctcgaaagc atatttccgc tcatgtgtct gactattatt gctatgctgt
      661 ttgactcttc atcaggtgac agaaaaaatt cttcatcttt tatctgttta aatgtattat
      721 ccccaagcga tttaagtttc ctgaattccg atctaacatt ttcgagataa tgtctgccta
      781 aattttcctt atccataatt tttattttat attaattatt tactgatctt ctgacaggtc
      841 tgtctgtaat tacttcaaag cccggaagta taagacttac ggtattcagc aaagactcag
      901 gaatgtattc acctttcggt tcagtttcaa ttttaatttt cgagatcttt tcctcatcat
      961 caaaatataa atacatcctg ctgccttcaa ttttatttgc gccgttagct ttgttttctt
     1021 catagacaaa atagacactg ctggaattat tatccacttc tatttcattt atcttatcat
     1081 cactgaattt aataattata tcatcacctc tgatctgatc atacctgtca ctgaagtatt
     1141 catcttcatt ggaagttaat gcaaatgacg attctgtacc gggcttgtca tttagttttt
     1201 tagaataaat attacttatt tttttacccg gcagctctgc ataaattgaa tctcccgtaa
     1261 gctgcagacg gtcctgccag actaccggtt tttctgaaag tgagacagtt tccagttccc
     1321 tgtaataaat tccgtaaccg catttcgcta aaaaatcatt cctgatcatt tctacatttc
     1381 cttcagctat ataataatcc ggtttatttc ggtatgcctt cattgtatca ctgtaaataa
     1441 aaagtgtatc tgcaatttcg ctttcttcga tctgaataag ccttgcattt tttttcaata
     1501 tcgtaaaatt ttcattttca aaattttcta tgtaatctcc ataaatgaca gaactgttat
     1561 tcagactttc gatctttaca ttacctgaag caacagattt tttttcaaca gaataatttg
     1621 tagcagtatc tgaagtgatc acagtagaat cactttcaat aaatacatta cctcttgcga
     1681 aagtcctgcc ggcactttta tagaaatcaa ttttatcagc tttgatgact gctgaatcag
     1741 ttgtaacaat tacatttcct tcagcaaaag aatcttccgt attccgcagg taagtcagag
     1801 tggaggaagt gatcctgtat tcgggattta ttatgatcac atctccggta aagacagctt
     1861 tgctttcatt aaaagtatag ataccgttat cggcgcggat agtagcgtta ggatctttca
     1921 aagtcacacc gccttcacag atcgcgcgtt tgtcatttcc aaaatatgtt gccttattgg
     1981 ttagcagtgt aagtgtatcc tgataaattt ttacatcgcc gcgtagctcg acacgatttg
     2041 aagtgatgaa ctgagtagct gagttgcaga aaactcttac attgccctgc acaaattcta
     2101 catcaccatt tgcttcacgt acattctcac cgttgactgt gcttcccgta agctctttgg
     2161 catttttcag ctcgatcttt tcctgtgcgc aaatatcatt cgatgatcca aacgtcaaaa
     2221 atattattac taaaaaaaaa tattttattt ttaaatctga tccgcgcata taattaaaat
     2281 gatttcataa gtttaaatat tttttattgc aagtacagaa ctttataatt ttttgaagca
     2341 ggttttgaat ttacatttta aattttgata ttagccggga tcaaaaattt taaaaaaatg
     2401 ttatgtctcc ttccctttgt ccaaaggata ggttgagagg tgtttgtaaa agctgtgatt
     2461 atcatccttg ttaaattttg taattgctaa ttctttcggt aacaacccct ccgctccccc
     2521 ttttctaacg ggaattactt ctgaattatt ctgtatcgtg cttctgtaat tttgattttg
     2581 cttcaaaaaa attcacttaa atattagact tgctcccaac cctcccctta ctaaggggag
     2641 ggcaagggtg gggtaaacta tagaaagaga taaaattcaa ttgtctaagc atattttgtc
     2701 aaaatttatt cattatataa tttgtttaga actgcaaaaa tcaatttcca ttaaacacac
     2761 cgctaacttt atatatttta taatttttca aattctgatc agattcaaat cctgtacctt
     2821 ctatagtttc atcgggagta gtaattctga caaattcatc tgagtaaact ttctgttcag
     2881 catttttcca gatgagtttt tcagttttaa gctgacttcc ttctgtgttt accactgtta
     2941 cgctgtctga aatttcaata tctttggtct tatcatcaat ctttcccttt ttgccggtaa
     3001 gtacggatac gattacactg tccctgtaaa actcaacttt tgcaccactg tcaattaacg
     3061 taaaaccttt agttgtatac attgcgatat gtccggcagt cagaatcgct ttgatatttc
     3121 ccgagtctga aaatacaact gtagaattcc agctttcctg cgtaggcatg tcgttgcttt
     3181 cagaattaat tgtagccggt ttaaaattat catcacatga ataaatgaaa attgttaatg
     3241 tcaaaaaaaa taatactgtt aaagacttta acatattata atccttcttc tataagtctg
     3301 tgcatatgga tcattccaat cggttttttg gatttgtcac cgacaataag ctgcatgatc
     3361 ttattttttt ccataatctc aagtgccgat attgccatca tatcttttga aataattttt
     3421 ggattagaat tcataatttc agcggcagtt atttcattca gtccgtcgag atttttttcg
     3481 agtaatcttc tgatatcacc gtcagtaatt atccctgcaa ttttggatct atcagtaacg
     3541 caggcacatc cgagtctttt tgaagatatc atataaatga catctttcaa ttttgaatta
     3601 acctgcacca ccggcacgtc aatatctttt gacattatat cccctacttt aagcaaaagt
     3661 cttttaccaa gattaccgcc cggatgtacc aatgcaaaat tatcagaagt aaattcattt
     3721 ttttccagaa gggcaattgc aatagcgtca cctaagacaa gggaaacagt agtggatgaa
     3781 gtcggtgcaa gattatgcgg acaagcctct tttttaactg atgcatccag tactatctca
     3841 gaagctttcg cgagttcaga ttcaagattg cctacgatag aaattatttt cactgacatt
     3901 gatttcaatg cggaaattaa atgtctgatc tcggtaatat caccgctctt ggatataatt
     3961 aatgctacat caccttttct gatcacgccc atatctccgt gaatactgtc agcagaattc
     4021 agaaagaaag cataagtacc ggttgaatta aaagtcgctg caattttata tgcaatgata
     4081 cccgacttcc ctattcctgt aactactact ttacctttgc atctatagat tagctcgact
     4141 gcttctgaga attttcctga aaagttttta cttgaaaatc ttttatgaag atcgtcaatt
     4201 gattttttct caatagaaac tacattcctt ccatttttaa tattcttaac ggtagaagtt
     4261 ttctgcatta atttaaaata acatttataa taggtattat aataattatt atagaccttt
     4321 attaaaatat aattaaaatt ggtaattggt taatatggtg aaaaaaggcg acgagatcga
     4381 agtaaagatc cttgaattaa attctgaagg gaaaggtgtt tcaaagatcg aaggtggatt
     4441 tgtaattttc actgacaaag ctctgcccgg tgatgaagca cttgtaagaa taagaaagaa
     4501 aaaatcaaaa tatgccgagg cagaattagt aaacctgatc acgccttcag ctttcaggat
     4561 agaacctgtc tgcaattatt tcggaacctg cggcggatgc aaggtcatga attatgaata
     4621 cggcaagcag cttgatttca agacaaacgc tgtaaagaat gcatttgaaa ggatcggaag
     4681 atttacagat ctgactattc ctcaagctat tggaagcaaa gagatttatt ttttcaggaa
     4741 taaaatggac ttctcttttt cggatgatga atgggtagaa aattatgaaa gacctgagag
     4801 aaattctgat gagaattatg tcagacagcc cggattcgct ttaggtctgc atgtaccggg
     4861 atttcattcc aaaataatca gcattgataa atgttatctg cattccgatc tgtcaaatga
     4921 aattttaaaa ttcacatcag atttttttaa aaaaaggaat acaactattt attcaacaaa
     4981 gacacacgaa ggttatctca gatttcttgt attaagagat ggaaaaaata ccggtgaatt
     5041 tatgctgaat ctaatgacaa actcttatga caaagaactt tttgctgaat attcagataa
     5101 gatcaatgag ctattccctg tcattacaac gatcataaat tccgtctcac gtaaaaaagc
     5161 ccagatagca gttgctgatg aagaatacat cgtaaaaggc aacggataca tttacgaaaa
     5221 gcttaagcgg ggaaaagacg gcagtgaatt aaaattcaga atatctgccg gatctttttt
     5281 tcagaccaac agtcatcagg cggaggtttt atttgatgaa acagtgagaa tgagcgatct
     5341 gaaaaaaacg gataatgtac tggatcttta ttgcggcgga ggttctattt cacttttcat
     5401 ttcagaatat gtaaataaag taaccggtgt agaattgatc cagagtgcga ttgatgatgc
     5461 aaagataaat atggaactga acggcattca gaacacagaa tttttagtat cagatattaa
     5521 ggactttctt acggatataa aaaatatagg agaatacaac aaagtaattc tcgacccgcc
     5581 gagatccggt cttcatccaa agatctgcga gatactttca gccacagatt ttgaaaaaat
     5641 aatttatgta agctgcaatc cacacacaca ggcacgtgat ctgcagctta tctgcgccgg
     5701 gggaaaatac atcatcgaaa agatacagcc tgtcgatatg tttcctcata cttatcatat
     5761 tgagaatatt gtgacactgg tcagagcgtg acattttaaa gtctgttccg gactgtatga
     5821 aaacgtaaca aatacctcta agactcaaag gcttaaagtt gtgaatttta atgcttttaa
     5881 acttcgtgtc tttaagcctt agaggcgatt ccaacattat caatcgagcc ggagaatcaa
     5941 aaaaaggggc taaatggaaa aaagattttt tatttcgaaa caaatagtgt atttttgtgt
     6001 agtacaactt aactttaatt ttagcctacc ggttttttta cagatctgta atagttttta
     6061 aatatacatt tcactgaaca ctattgatga atgacttttt aaaagtacat ttaatcatta
     6121 atttaaattc gcaatcaaat tttcaaaata agataaaaaa tatagaaagt atttactttt
     6181 tctaaagctt cttaaaaagg aaaagaaagg aaaagacatg caaacaactg atatgcaaac
     6241 agcaaataaa gacaaaaata atattttatg ggtggatgat gagatcgaat ctctgaaatc
     6301 caacattatg tttctgaaac aaaaaggtta taatgtacat gaagcgacaa acggtgagga
     6361 cggtgtcaat ctcattaaga aaaatgattt tgacctggta ttcctcgatg aaatgatgac
     6421 aggaatgggc ggactgcaga ctctgattga gatcaaagac ctgaagccaa gtcttcccgt
     6481 tgtgatggtt acaaaaaatg aaaccgaaag tctgatggaa gatgcgatcg ggaaaaaaat
     6541 atcagactat cttattaaac ccgtaaatcc aacacaactt ctgctcgtct gtaaaaaaat
     6601 gctggagtcc aaaagattaa aaggtgatca ggtttccagg gattacatac aggaattcaa
     6661 caagctcaca atggcaatgt ccgataatcc gggctgggaa gaatggatcg acatttatct
     6721 gaagcttaca ggttttgata tggagctgga tcatcatccc gaattaggat taaggcagac
     6781 tatttttgat cagaagagag actgcaatat tgaattttca aaattcgtag agaaaaatta
     6841 tttaggatgg gtaaattcca ccagtgacaa accaatattg tcaaatgaaa ttgtagataa
     6901 attcataatt cctgagctgg atacaaatga atcagttttc ttatttgtga tcgactgtat
     6961 gagacttgat cagtggctcg agatggaaaa atatctttat gattatttta atattaagaa
     7021 atcatatcat tattccatac ttccaaccgc aacaccttac agcagaaatg caatttttgc
     7081 agggctgttt ccttctgaga tcgaacatca ttatcctgaa ctctggaaaa aaaatgatga
     7141 cgatgagaac agtaagaata attacgaaag agaattcctg ataaagcttc tggagagacg
     7201 caggatcaaa ctcagaaatg aagttaagta cacaaagata atggattcaa acttcagcag
     7261 aggaatcgaa aataagatca tgacatattg ccagaatcat ctcaatgcga tcgtaattaa
     7321 tttcgtagat atgatcgcgc attcgagaag tgacaatgcg atactaaaag aaattgcacc
     7381 tgacgaagca gcttacagat cactgactgc atcatggttt gaacattctt cattctttgg
     7441 gatgcttaga caactttcca caaagaaaaa tatcaagatc ataatcacca ctgatcacgg
     7501 aagtataaga tgtcttcacg gcgtgaaagc aatgggtgac aaggatactt cgacaaatct
     7561 aagatataaa tgcggcagga atgttaaagc tgatgcgaga aatgcaatta tgataaagaa
     7621 tccacttgag tataaacttc cgaaaagaaa cggaattata aattacatta ttgcgaagga
     7681 agatttttat tttgtatatc cgactgatta tcacaagtat ctgaatcagt ataatgatac
     7741 atttcagcac ggcggaatat cgatagatga gatgatactt cctgtaatta cactggattc
     7801 aaaagtgtaa tggagaggat tgttacatcc gtttcaacgg attatactga agaagcggga
     7861 cgtgtatatg cgtcaacact ggatccggga accatcaccg gtttatttgg aaatctcgga
     7921 tccggcaaga cacagtttgt aaagggagtc tgccgatatt ttaatgtaaa agaaattgta
     7981 aacagtccta catttatcat aaaaaacgag tataaaggaa aagatcctgt ctcagaaaat
     8041 gtaattaata ttcaccattt cgatctatac agaatcaaca atataaacga gttaacacaa
     8101 ttaggattaa atgttattga taatgaaagc actatcttat taattgaatg ggcagaactt
     8161 gcagaagagt attttcgcgg gaatatcagc aaaatatttt ttgattatgg acaaaaagaa
     8221 aatgaaagaa taataaagtt cgcataaaaa tttgaatata cttttcttag acacttcatc
     8281 taaaatttcc gaaacagcat tttatgaaaa tgaaaaaatg atcttccatt taattttaaa
     8341 agaaagtgac ggagcggatc aggtagttta taatttaagg caatcattta aagaatttaa
     8401 gcttgatcca aataacattg aatgtttaag tttatcaaac ggacccggat catttacagg
     8461 attgagaatc ggctctgctg ttgcaaaagg aatttgtttt gtaagcggtt gtaaatttat
     8521 agaaattcct actctggaca tactggcaaa cattattcct tcaggaaata gtatcacttc
     8581 tttaattcca tcaaatccaa agacttcaga attttatttt gcaaaatatg aaaaaagtgg
     8641 aaatgattta aaaagaactt cagattttga ttttggaaat ttacatgaaa ttttaaaaac
     8701 tgattcgcaa tttgtaataa aaaaggaagc ctttcaatat attccggaac caatccaatc
     8761 gaaattcttt gaataccccg acgacgcagt tatgaggtca caatatgaaa ttacattaaa
     8821 aaagatctca gacaatgaat tttcagatgt agaaaattcc acccctattt atatccgtca
     8881 atttatcccg aaaaattgat tttaaattgg tacatatctt ttttatttta atccaaataa
     8941 aaattatggc ctggtttaaa agatcgaaag aaaacatttc aacactcgaa cctaaaaaag
     9001 aagtatcaga cggtaactgg cttaaatgct cggaatgttc tgaaatgata cacaagaaga
     9061 cattagaatc aaatttttat atttgcactc actgcggcaa tcacttcagg atcggcagca
     9121 gtgaatattt taatattttg cttgatgaag gttcctttaa agaaatggat aaaaaggtga
     9181 agtcagtgga tccgcttaac tttgaggata caaagcctta caaaaaaagg attgatgata
     9241 caattaaaaa cactgaacta tatgatgcga taagaacagg aaccggaacg ataaactcga
     9301 ttgacgtcgt gattggatgc atggatttta attttatagg cgggagtatg ggaagtgttg
     9361 tcggtgaaaa gatcagcaga gcaatagata aatctattaa aacaaaatat ccgcttatca
     9421 taatatcatc cagcggtggt gcaagaatga tggaaggagc gatctcatta atgcagatgg
     9481 ctaagacaag tacaaaatta gccaagctca gcgaagaaaa aattccatat atatcaatta
     9541 tgacaaatcc tacaacaggc ggatcgacag cgtctttttc aatgctggga gatctgaaca
     9601 ttgcagaacc aaaagcactg atagcatttg caggaccccg tattgtcaaa caggctacag
     9661 gcaaggatct gccgcccgga tttcagaagt cagagtttct actcgaacac ggattcattg
     9721 atctaatagt aaaccgaaaa gacctgaaag aaaagcttac acaaattttc aatcttttac
     9781 aaaattaatg aaggttataa aagaagtccg cgcagttcag cagatttccg gattttacag
     9841 atgtaccggt aagaaagtag gattggtacc gacgatggga tttctgcacg aaggacatac
     9901 ttcactgatg ctcaaagcaa aagacgaatg tgatatactt attgtcagta tttttgtaaa
     9961 tccaactcag tttggcaaag gagaagattt tgataaatat ccaagagaca tagtcagaga
    10021 ttttcatatt tgtgaaaagt caggagctga ttatattttt aaccctgaag tttctgaaat
    10081 gtacggcgaa aataatttta caaaagtatc attatccaat gtcacagaaa agcttgaagg
    10141 agatatcaga cccggacatt ttgacggagt tgcaactgta gttacaaagc tgctgaacat
    10201 atcaaagccg cacaggatct atcttggtca gaaagatgct cagcagaatg cagtattaaa
    10261 aagaatgatc tccgatctta atattgatac tgatctgata gtttgtgaaa caataagaga
    10321 agaaaacggt cttgcaaaaa gttcgcgtaa tacttttctc acaaatgaac aaaaggaaaa
    10381 agcctctgta ctttatttta ttttaaatga aggtaagaga cttataatgg aagaaaaagc
    10441 aaatgattca ggaattgttt atgaatttgt gaaagatata ctcaaagaaa agacacctga
    10501 atttgaactt cagtattata agatcactga taatatttta cttgaagaaa taaaagatcc
    10561 ttcctcattt aaaggtgaga tacttatttc attagcctgt aaggcgggaa acacaagact
    10621 tatagataat attattttca ataaataata ttcgatttta attttttata taataaactc
    10681 agagctttaa atgaacagaa atttagcaac agtaataatg gctgcaggta aaggaaaaag
    10741 aatgaataat ccggataagt caaaagtaat gtttgagttg aacggtaagc cgatgataga
    10801 atatgtagta agccttgcag aacaaataaa ttcagaaaaa atcattccca ttgtcggtca
    10861 tcaaaaagaa tttgttatga attttttgaa tgacaaattt tcaaatattt cagataagct
    10921 cagctatgca catcaggatg ttcagcttgg caccggtcat gcattaatgc agacacgtga
    10981 acaccttagt aattttgaag gtgatgtatt gatattatca ggtgatgtac cgttgcttac
    11041 agagaaaact ttgagatctt ttattaaatt tcatgaggaa aataattttg aagccagctt
    11101 gatctctgca attcctgatg atccgacagg atatggaaga gttttaagaa atgaagaaaa
    11161 tgaattttac gatatacgtg agcataaaga ctgcaatgaa gaagaattga aatgccgtga
    11221 gatcaattca ggaatttata ttatagacag caaaattctt tttgaagctc tcaaaacaat
    11281 caaaacagat aatgcacagg gagaatatta tctgaccgat atatttaagt atttcagatc
    11341 aaaaggaatg aagacaggtg cgtttgttac agataatgta attgagataa caggtgtgaa
    11401 ttcaactgaa cagttgaaag atctcgaatc aaatatttag ttcacattct aatataaatt
    11461 aattttaaaa actaaattgt cagaacttat agtttcagtt tcagggatca gaggaattac
    11521 aggcgacagt ctgactcctc aaaacattat aaaatacact tcagcatttg cagaatactg
    11581 cagaaagaat tctaaaaaaa agaaattccg aatagctgtt ggaagagacg ggagacttaa
    11641 cggtgatata actgaaggta ttgtaataaa tacactcagg atgtgcggag ctgatgtgat
    11701 aagcatagga attgctccga ctcctacagt tcaaatcgca actgaagata tgaaatgcgc
    11761 cggtggtata tcaataacag catcacataa tcctcagata tggaatggac tgaaatttct
    11821 gaatcctgac ggaactttcc ttgacggaga tcagatagaa gattttaaaa aaattgcaaa
    11881 gtctgaaaag tttgaatatg ctgatctgct gcatacaggt aaaattatat ctgataattc
    11941 tatgtccgat catcatattt ccaaagtact gaaactaaaa attcttgatc tgaaaaagat
    12001 cagatcacgg aaatttaaag ttgtagtgga tgcagtaaat tcttcaggat ctgtaattgt
    12061 cccaacgctt ttaaaagaac ttggctgtaa agtaatggaa ttacattgcg acagaagcgg
    12121 tatatttcca catatacctg aaccgcttcc tcaaaatctt aaatcacttt ctaaagctgt
    12181 aaaaaagttt aaagcagata ttggaatttc ggtagatccg gatgctgaca gacttgtaat
    12241 gatcatggaa aacggaaaac catttggtga agagaataca attactatga tagtaaatca
    12301 tgtacttaga aatcaatccg gtaaaaataa ttctgctgtg gtaaatttgt caactacgag
    12361 agctgtggag gatttagctg cggttcataa ttccaaagta ttccgaagtc cggtgggaga
    12421 gatcaatgtt gtaaaagaaa tgatgaagag gaaatctgta attggaggtg aaggcagtgg
    12481 cggcgttatc tatcctgagc tgcattatgg aagggattcg cttgttggta tagcattagc
    12541 tttgaatgaa ctagcgcagt caggattaag cctgagtgaa tatttaaata gattgcctgc
    12601 atatactatt tccaaagcaa agattgaaaa tgtaaaagat cctgaaaata tattaaagca
    12661 gttggaaaaa aaattcagac gcgaaactaa tataacagga atacgaacag atgacggtct
    12721 gaaaattgat tttaatgacc actgggtaca tctgagaaaa tctaatactg aaccaataat
    12781 aaggattata actgaagctg ctaaaggact ggacgctgat aagatcaata aaaattttat
    12841 tgattatata attaagctga ataaaaatta aaataataac ttttactttt tctatatttc
    12901 acatattttg attcattaaa ttttttaaaa caaaaaacct ataacattat gaaaaaaatc
    12961 ctattaacgg tgattgttct ctttacagca tttacccttt attcatgttc atctgtaact
    13021 gaaatgtcag gcacatggaa aaaaccgggc acgactgcaa caaaatacaa taagatagta
    13081 gtgatgggag tttcaaaaaa tcttgtcaca aaatctacag tcgaaaatgc aattgtaagt
    13141 cagttgaaga agaacggttt taatgctgta gcaggagcaa atgtattacc gacatctaca
    13201 tttgatgcag atgccgacgg aaaagcagat gatgacagta aatcaaagat cgctgaaaaa
    13261 cttaaagagc taggtgtaga cggtgcttta gttttatcac tccttgataa aaaagaaagt
    13321 gaaagatatg taccaggtac aacaatgtat tcaccttaca attcatatta tcctttttac
    13381 ggatattacg gcggagcata taacatggta aacactcccg gatattatga gaaagttaca
    13441 aattactttt taacaagtaa cttttacaat gtaggtacag agcagctggt gtggtccgga
    13501 cagtcagaaa catttaaccc gacttcatta agtgattttt caaattcata tgctgagaag
    13561 gtcgttgatg aattattgag ttcaggaata gtcagaaaat aagcttcata atttttttaa
    13621 atttcttttg gggaaaacct tttttgtgtt ttcccctttt ttatttttaa cctgcctgca
    13681 aaaaatctaa aatcattata taatccgttg taacttcctt ctaatatttt taatttattt
    13741 ctattcataa aattacttct acactgtatc tcttttagtt atacaatttc tttatgaaaa
    13801 ctaaaataag ttttttatta aacgatactc ttattgaaac cgggctgaat cctgctactg
    13861 ttcttcttga tttcattaga tccaatgatt ttacatctgc aaaagaaggc tgcaaagaag
    13921 gtgactgcgg tgcatgcact gtcatttgcg gtaaacttaa taatggcaaa ataatttacc
    13981 gtacaataaa ttcctgtctc gtacctgtta tgaatattaa tggtgcacac attgtaacca
    14041 ccgaaggtct gaataatgta tccggaattc tgacaccggt tcagaaattg tttttaaaag
    14101 aaggagcttc tcaatgcgga ttctgtactc ccgggtttat agtttcgctc acgggatttt
    14161 ttctatccga caattttaat gatgatattt tagattcaat tgacggaaat atatgcagat
    14221 gcacaggtca taattccatt atcagagctg cagaatattt ttcatctgag ttaaaagata
    14281 ttcctgaaga ttttgattgt agaataaaat atctcattga gaaaaacatt attccggaat
    14341 tttttctgag tgcaaaagaa aaattaattt atatacaaaa gaatattgaa ccaaagaata
    14401 ataaaaacgt cagtccaaaa tatttcatat ccggaggtac agatcttttt gtccaaagac
    14461 ctgatgagat gctttcatct gacgtttctt ttctctcaaa ggaaaacagt caagaactaa
    14521 tttatgaaaa agacggaagt tgttttattg attcaacctc aacagtaacc gatatatttg
    14581 aatctaaact tattcaaaaa ttatttccga tcatatctga atttacaaaa ttatttggct
    14641 ccactccaat ccgaaacagt gccactatcg gtgggaatat caataatgca tcaccaatcg
    14701 gagacatgac agcttttttt atggctctcg atgcagacat gatattatcc gacggaaatg
    14761 aaagtcgtga agtcagtttg aaagattttt acacagggta taaaaagact gtcagaatgc
    14821 ctgatgaatt tgtaaagtct gtaaaattta aaattcctga cggtcaattt tattttaatt
    14881 ttgaaaaagt ttccaagcgc acttatatgg atattgcctc cgtaaatact gctctgtttc
    14941 ttcaaatgaa tgaagatgta attacaaaaa ttaatatctc tgcaggaggt gtttttgaaa
    15001 ctccgaaatt tctttctatg acttcagatt ttttgactgg aaagaaaata aatacagatg
    15061 taatactgga agttcttgag attactgatt cagaaatttc acctatcagt gatgcaagag
    15121 gcagtgaaaa atataaaaga cttcttttga aaagattaat atggacacac ttcataaaat
    15181 tatttcccga attaattaac atcaaagaga ctgtatgaaa aactatgata taatacagca
    15241 tgtaacaggt aagtcccagt tcatcgatga tattaatgtt ccgaacggta ctttgtttgc
    15301 taaagtgttt tattcaaaaa atgcacatgc aaaaattact tcaatggatc tttctgaagc
    15361 tgagtctata aaaggagttg taagaatttt aacttacaaa gatatcccgg gagtaaatca
    15421 ggtcggaggt atagttcttg atgaaccttt attagccgaa gatgaagttc attttatcgg
    15481 aatgcctgta gcacttgtaa ttgcagagtc tatgctcatt gcaagagaag cttcagataa
    15541 aattaaaata gaatacaaat ctcttcacgt gatcactgat ccgagggaag ctttttcaaa
    15601 aggtctgttg tcaattccac ctaggatttt tgaatcagga aatacatatg aggcttggaa
    15661 agaatgtgag ttcttcgaaa ctggtacatg tgaaacaggc ggacaggaac atttatatct
    15721 tgaaacgcac ggtgcaatat ctatcccaat ggaaggcggc aaggttaaaa ttatttcttc
    15781 cacacaaagt ccgactgccg tacaaagagt tacatccggt attctcggaa ttccaaagaa
    15841 catgatcgaa gtagaagttc tgcggcttgg cggcggcttc ggtggtaagg aagatcaggc
    15901 atctcactgg gctgcactct ctgcacttgc tgcatgtatt cttaaccgtc cggtgaaact
    15961 tgttttatcc cgacatgatg atatggtaat gaccggcaag cgaaatccat tttcatctga
    16021 ttttaaaatt ggtttgaaca gagaaggaaa aatacttgca tatgaagtta cattctatca
    16081 aaactcaggt gcagcggctg atctctcacc tgctattctc gaccgcgcat tgtttcactg
    16141 tacgaacagt tactatgtcc cgaatgtaaa agctaccggc tattgctgca aaaccaactt
    16201 aactcccaat actgcattca gaggattcgg gggtccgcag ggaaagtttg taatggaaag
    16261 tgcaatttac aaagccgcag aaaaaatgga tatggatcct tttgaaattc agagaataaa
    16321 tcttttaaag gagaacgatg aatttcatta cgggcaaaaa gctcttcatg taatggctga
    16381 aaagacctgg gatcaatgtg ataaggaatt taatattgat actgttaaaa agaaaaccga
    16441 agaatttaat tcggaaaata atttatataa aaaaggattt gcagtaattc ctatctgctt
    16501 cgggattgct tttacaaata cacgaatgaa tcaggcaaat gctttagtta atatttacac
    16561 agacggaagc gttggattca gcactgctgc agtggaaatg ggtcagggtg taaatgctaa
    16621 aattcgtgaa gtcatctcaa gagtattttc cataagtcat gacaggatcc ggagtgagcc
    16681 gacgaataca acacgttcgg caaatacatc accgactgct gcaagttccg gagcagatct
    16741 gaattgtaat gcagtttatc tcgcatgcaa taaaattctt gaaagattag tacagtcagc
    16801 agctgaagat ctcggtgaaa aaaattccgg gaatataatt ctcaaagatg aaaaatttta
    16861 tcttaatggt gaagagacag atctcaactg gaataatgtg atcgaaactg cttattccaa
    16921 aaggataagt ctttgcgatt atgcacacta ctcaacacct gagttatgtt ttgataataa
    16981 aataaacaaa ggaaatccat tcagatatca cgtttatggt acagcgatta ttgaagtcac
    17041 tgtagattgt ctgagaggga tttataagat cgattcggtc aaagccgttc atgattttgg
    17101 aagcagtctg aatcctgtga tcgatcttgg tcaggctgaa ggtgcgctga tacagggatt
    17161 aggatggatg acaattgaag aagtgataca tgataaaaaa ggcagactgc ttactgatct
    17221 gctctccact tacaaagtgc ccgatgtaca tttcgcgcca aagaatatcg ttataaaatt
    17281 tttgaatgag ccgaatcctg ttggtatatt caattccaaa gctatcggcg aaccgccttt
    17341 gatgtacggc atcggtgttt attttgcttt gctcaatgct gtcagagaat ttaatcctga
    17401 tgctgatctc gaattcaatg ctccattcac tcccgaaaaa gttttactga aattatatga
    17461 aaagaaaaat atgactgaac ctaaacctgt tgcagtaaat cattcagaaa gtatttaatt
    17521 tcaaatttat tattccacta attttacttc tatgaaagac ctttctttat ggaaattcat
    17581 tcaatctgaa ttaataaaaa ataacagagt aattctgttt actgtagttc atcatgaaaa
    17641 gggttcaccc ggtaagacag gttttaaaat ggctgtttcg tctgaaggaa aattctcagg
    17701 atctataggc ggcggggtaa tggaatataa tttattgaaa tcctgtgaca aattatttct
    17761 gtcaggtaaa aaaatcaata ctgcagaaaa atattttcat tataaaggaa aactgcccgg
    17821 taagaatatt ctgtctgaaa aagcttcggg acttatttgt tcgggttcac aaattattgc
    17881 ggcaaattta tttacacata aagatcttac tatggtcaaa gaaatcagat cagcatatcg
    17941 attgggtaaa aatgtaatat taaatttgga cagcacagga cttactgtca gacctattga
    18001 aatttcagag aaaagacgat ttgaattcac cgcttcagtc aaaggattgt ggagctatga
    18061 agaaaattta agtttaacag atacaatatt gatagcagga tctggacatg taggaattgc
    18121 tttgcgcaaa gtcatgtcag agctggactt tagaatttta atttatgatg acagaaaaac
    18181 tccattattc aaattcaaaa aatttaccgg ctgcagattg atcgttgatg actactcaca
    18241 tatcctgact catttaagaa atattaaaat taattatgct gtaattgtaa caacgggttt
    18301 tgaaagtgac aaagtggttt taaaaaattt aattaatatg aatctgaaat atcttggact
    18361 gatgggaacg aaagcaaaga taaaaaaaat atttaatgaa gctgctgagg aaggcatttc
    18421 aaaagtatta ttagaaaaag tatatgcacc ggtcggaata gatattaaca gtaatactcc
    18481 ggaagagatc gcaatcagca ttgcggcaga gatcattaag atccgtaacg gctgattatt
    18541 taattacctc taagactcta aaactcaata ctctaagact ctaagactct aataaaaaaa
    18601 ataaattatt aatctgatat aatagcttta ttaaatagtc ttagcagagt gacgtgcttt
    18661 tcggactctg cggaatttta aaattgattt cgtttattga aaaaactttg agtctttgag
    18721 ccttagaggt atccgccaaa acttgaatgt tatttaaatt tcaattcata attttcctta
    18781 ataaacttat cagccttttc aggataatgc tttttcaaaa acttcaaagt cgaccgcatt
    18841 gcacggatca caataatatt ttcatcatct gtaaatacct tcatcacttc caaagcctta
    18901 tccggattgc gtttcccaaa gctgttattg aatgacatta taatattcca tctgacataa
    18961 ggatctttgg atcggctttc cttttttaaa aatgcagcag tctcttcagg aaatctactt
    19021 ccgtaaagtg aaccgagtat aaatggtccg agattttttt tcacatatac cgaactgtca
    19081 ttcatcaggg atctgagtat tttaaaacct gtcttgtatt tcttttcatc tctgactcca
    19141 agtgcggaga ataccactgc acgtcttaca ttttcagatg gatgcttact ccattctttc
    19201 aggtcggtta aaaaatcttt atgatagtaa agtgtttcag caaatgcatt tcctgcatat
    19261 tcccttactt cccagttcgg atcatctgaa attctgtgca gcatttcttt tactttttcc
    19321 ggagcatgat catatcctct ccagatcaaa gagactccaa tctcttttgc attatcttct
    19381 ttccgtttac aaaagtacat acctgtttta taaaactcac tttctctatt accttcttta
    19441 tctattatct ctctgacaat ctccctgatc acaaatcttt tatcctttgt ctttgccgta
    19501 cctgcatgat cagaactgat tttttccagc ataccgatta ttttatcaaa atcttttgaa
    19561 ccgataagtc ttaatatttt tttcttatcg ggttcattta agagagagtc tttttccatt
    19621 ataaagttta ttttcagaaa gtattaattt aacaaaatga actactcatt taaatagtga
    19681 tttggatgag attatttagt tctatgatct ggaattctaa agctaaaaaa atcccgcaaa
    19741 gaaaattatt ctctgcggga tactggttat ttaaaatttc atatttgtaa tttgaaatta
    19801 gtaatttgaa attatttcag aagcatcatc ttcctcacct gctcaaaatc ttccgccttt
    19861 atcttaaaat aataaattcc gcttgagagg tctgatccat tgaatgagat tgtataactt
    19921 ccggcagtct tatagtcatt taccaatgtc ctgatttccc ttccgagcat atcaaagatt
    19981 tttatcgaaa ctaaaacatc tttcggaaga tcgaatttaa tgtttgtagt tggattgaat
    20041 ggatttggat aatttgttat tgaaaacata tcaggtaaaa tattattttc cgattcaatt
    20101 aaatcttctg tctccggacc gcattcctga ttatatccgg ttaacaatcc gcgttctgat
    20161 tttaacgacc tgttgccgcg attgtctttt gcaagtatct tataagaata ggttaataaa
    20221 gtatattcgc aataatccgg aatcgcagga atattatcat ataatgtaac attgctgtca
    20281 acatattgcg tagttgtcga actcacagtt gttataagtg aatacacagg ttcaatatca
    20341 catactgttg tgtcagctct gtaaatctca taaacgggtg taatcagcag tggtgaaaca
    20401 gcgggagcgt cataaaagtc aggttcaata tttgaatccc atgttacaac cggataaaat
    20461 ttatagcttt ccggctctcc aaaatattgc ttaccaactt ttaaattctt tggttttgaa
    20521 ggaggaagat cgatgagtgc atcttcgtca ttgtaataaa tttttaaagt aattgctcca
    20581 tatgaatcct gactttgaag aactattgtt aagccattat tagaaagatc ctgacaggaa
    20641 cgagtagtag ggttactata tggactgaat atttcatcgt atccaatatt atatccatct
    20701 aatccatccc catgaaaatc ccccgttatg aaatagtcat ttgaagctga tgagcaaaaa
    20761 tcgtcagtaa ttaattgtgc tgaccaagtc gggtaagtct gaagatagta tttattgaat
    20821 tcgtatactc cgctgtccct atttccattt aaattctttt gaataccaaa agaacctaaa
    20881 tatggatctg aaactgttct atccaaagcc cagttatatc tcccttctga atttatcaaa
    20941 tcaaaagcaa aatgcctgtt tccatgaatt aaattatatc cgttgacatt attattacag
    21001 ttccttggag act
//
LOCUS       DAORVQ010000035        20827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_990_length_20827_cov_5.052089, whole genome shotgun sequence.
ACCESSION   DAORVQ010000035 DAORVQ010000000
VERSION     DAORVQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 20827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20827
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_990_length_20827_cov_5.052089"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1187
                     /locus_tag="PLX80_04730"
     CDS             63..1187
                     /locus_tag="PLX80_04730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20135.1"
                     /translation="MKKIAFVSSNKSEWGGSEFLWYNTALIFSEKGNDVLVTIPRWKK
                     IPEQIDFLKSKKIPTYFNTDTSTYKKLFNRVLPSSMQFDYSNDGNKCLREFSPDLVVI
                     NQGGNTGGADLMEYCINSGLKFVTISQAANEAKWPSDSLNKRLSEYLPKAIRNYYVSK
                     ANIKLTELQTGQKIDNAEIIFNPFNVSYNNRINYPEVRENYFIANVARHEFFAKGQDI
                     LFQVLNEKKWRERNLIVNLFGKGEHSFQIKKLKDYFGLDKVQIKGHKDPEEIWKENHA
                     MILTSRYEGLPLALVEAMLCGRVSIVTDVSGNREVLTEGKTGFLARHPDPESVSEALD
                     RAWQRRYEWKEIGEIAREEIRKIVPENPSILFYEKLNSIV"
     gene            1180..3627
                     /locus_tag="PLX80_04735"
     CDS             1180..3627
                     /locus_tag="PLX80_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20136.1"
                     /translation="MSEEKLKIKSETITEKLKFPNLEVKYKICIVSAILPPAYGGAEV
                     AAFKYALRLEKDPESNILVIGWDRTGGYKESKNKFEFVVPVTFSENPKDAKGILIYFQ
                     QYHHMWNCFTKLLKPMWKHRKEYDYIHNFNSGFAFNRVSILIAKILGKKIVTETSLVG
                     DDDPLSLGRFLDWKDYLKPKFIRYLFYKLADRYVSKSNVITDIYRKSEISMNKVVQIP
                     YSVDINKFYPPEPERKKKLREKFKIDENDVVVIFVGGINVRKGVHLLVNSFVDVHKNF
                     PSSKLLIVGPTYKYNQQYIIDIKNKISENSLEDKIILTEDNVSNVEEYMQCADIFVLP
                     SRQEGFPISIIEAMSCGLVVIGSDIPEISKAQIVNGVDGFTFSLSKPEELTFILKKLM
                     GNKDELIKIGNEARAKVVKYWSDEIVDKEYKKLYSSINFVLPINTYNNINTKKEREKI
                     KILYTIPNFNTAGSGKALINVISRLDRNVFEPFICCRHDRGDLFSKVQELDIPVYITD
                     FTVSTKPRIKGFKNVLKLSKNFKNIDPDIIHSYNYSDDYSEALASKLSRIKWIYTKKN
                     MGWGSNAWKIRSKLSSAIIPQNQEMVETFFRGIKEQYLIPIGIDIQEFKKTSRDESII
                     TRYKLYNSNPIIITLANVIPIKGIDYLIKGFLLALEDYKDAKLLIIGDDVTDYAKDLK
                     KKTIDAGLEGKVFFTGKQKDIKPFFSIADIFILSSNKTGEGGPISILEAMASGIICYG
                     SDVPGIRDQFREFEDQLFESENPGSIANKIMNYMRMNPEVKQEKIISQLEFVKKYYSI
                     ENEVDKLQKLYLKLIKT"
     gene            3639..4694
                     /locus_tag="PLX80_04740"
     CDS             3639..4694
                     /locus_tag="PLX80_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20137.1"
                     /translation="MKGIKFLRYLPHYLRKKFKEKIIVIESDDWGLERAMNIDSINFL
                     KKKYSEEKFTRWTFDALETNEDIELLYEVLGSFKSNFEYPPVITANFVTHNVDYDSSD
                     DLKFIPISKGFNKDSEDVRELYKCGIENNFIYPQLHGFSHYNLSELTKYFYTKEGQEA
                     FENSFFTGSTTIKGNLKFLTGELCFMNKDTKIKESIDELCKIFNVESETIIPPNYILS
                     KQNIDDLKNNGIKMIQSSNRLVQSDGSRYSFPYFRIRNGLVWSVRNARLDPHIDYGFY
                     HDECINDIGKAFDNKLPAIIDFHRVNFSGKFTPLYRDKTLSEMKKLFECVYKKWPDVK
                     FLNSSDLNKLIWQQETR"
     gene            4673..5329
                     /locus_tag="PLX80_04745"
     CDS             4673..5329
                     /locus_tag="PLX80_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY20138.1"
                     /translation="MATRNQISFLKNNIDIIIEPVLIVGSKHYEFDKEDIRERLKEMG
                     KTDVTGIDISEGEGDDYVVDITDSECEFLKEKSAKYSTVICMEVLTHVRNPFKAAENL
                     SGFLKEGGTAILSECYTRKISKMPIDLWRFTYDGTKELFKDLKFSDERARVSLTREKD
                     EKLLPLKYPLPQLMAQKSDDENAAGYFLRRLHRKYFAKGIFRISRLLPETTIYSIGKK
                     "
     gene            5353..6066
                     /locus_tag="PLX80_04750"
     CDS             5353..6066
                     /locus_tag="PLX80_04750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20139.1"
                     /translation="MGKDNMYENLKDRVAFSLSKRNLKKILSSEDIDEVLKSIEIYKG
                     RGFYDKIRLAQEHTEIRDLCKKVVEHKPKIITEIGTWNGGTFYVWARTNPHAEKIISI
                     DLPDGDYGGGYDEKRIKFFSEFASDRKNTSLSFIRGDSKSNSTVEMLKSVLGDKTIDF
                     LYIDGDHTYDGIKKDFEIYSELMSSDGIIGFHDINTHKDGYGVIQFWNEIKQNYRYEE
                     FIKPGSRVMGNGLIYLKKD"
     gene            6068..7054
                     /locus_tag="PLX80_04755"
     CDS             6068..7054
                     /locus_tag="PLX80_04755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQY20140.1"
                     /translation="MPEVSIITPLYNGSNTLEETADSVLAQDFKDWEWIFFDDGSTDD
                     TIEIAKKIQSKFPDKIFLYTHDNNSNHGTAFSRNRAFEKSKSDIIAFIDQDDIWYENR
                     LSHQINIFRNKTECAMIWGPALYWYKDRSFVQSVGLNGKGLKSGIYDPPDFVEIFLSN
                     LKGTPLPSASLVRRKEFVNVKGYEESIKGSEDIVLWLKLSEKFQIYYDDEVLIKYRKH
                     QDSTLRKASDSGKMDEWNLVFYKWVIDFLKRTSSNESILEENEFAFYKNLKKISGKEN
                     YLKSRKDLYNRLNEYPDIWKKYSGDYLLDLVLPFDTASRISAKLRFDLFKKK"
     gene            7055..8455
                     /locus_tag="PLX80_04760"
     CDS             7055..8455
                     /locus_tag="PLX80_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQY20141.1"
                     /translation="MRTEIIPYKQQFYKNGNESGLDLKAICIFSALGFFLGRDTYYKN
                     LEALQPASDYNIDETGNIISAEEYWKWHYSPREMNLKQATEEFADLFEKLIHENLKDK
                     KVIMPLSGGIDSRSQAAAIGNKFDTKSYSYKFEKSFDETKYGRLISEVLGIPYKEYVI
                     PEGYLWDVIDELAEINQCYADFTHPRQMAVIKEVSKFGDVFFLGHWGDVLFDSMGVDE
                     ELRFDELVNTVLTKIVKKGGIELAEMLWEHWELEGKFRNYLEDRISELLSEIKIDNAN
                     SRIRAFKSMYWAPRWTSANMNVFSNFHPIFLPYYENEMCEFICNIPEDLLAGRKIQIE
                     YIKMKNPELAKIPWQSYEPLNLYNYKNFQNIGNLPGRVVRKVNRIINEDVLKKKKTKS
                     NYEIQFLGKNNDSQLKKRLFENDDLKNILPEKIVKDIYGKFINEDSKKYSHPVSMLLT
                     ISQFSNSLSFKQRESE"
     gene            8452..9636
                     /locus_tag="PLX80_04765"
     CDS             8452..9636
                     /locus_tag="PLX80_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20142.1"
                     /translation="MKVAIYSGNIPSTTFIENLIRNLSNSGFRILLFGKKIRDVKYNE
                     NVEVHNTPNNSIIILFFILKESLKLFFKSPNKFSKLLKYISSSDTGLKSKLKELGMML
                     PIINNEPDIFHIQWAKTVQKNSYLFNLMKCRFALSLRGAHINYSPLNDESLAESYRKY
                     FPKLDGFHAVSKAISIEAQKYGAAPEKIKVIYSSVQDELLQIKTESKINRSEVTNIIS
                     IGRYHWKKGYHYAFDAMKILIGKGFNFQYTIVAQGDVPEEILYILSDKELKSRVVLKN
                     GMAHDELMNELKKSDMLLLPSVEEGIANVVLESMAIGVPVISTDCGGMNEAVSDFENG
                     MMAKVRDPNMLAQKIIEYSELSENKKEQMKLIAKNTISEKFTDKKQIDGFVNFYNSLL
                     YS"
     gene            9641..10843
                     /locus_tag="PLX80_04770"
     CDS             9641..10843
                     /locus_tag="PLX80_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20143.1"
                     /translation="MKIGLVLPEVPQYSETFFNFKINGLVESGFEVLLFTGNKKNSKN
                     RANEKVKVIPAYPVYRSSSIYQFLLFIYVVSFRFIFSLKKTLKLISLESKVGKNFSER
                     MKSVYLNAHILKENIDVLHFGFTTMAIQRENVAKAMNAKMSVSFRGYDINIYPLKNPD
                     CYKNVWRNIDKVHTISNYLREKAVSLGLPEKTPYIKITPAIDFEKLSQNKMRTDISVP
                     KILTVGRLNWIKNYETAISALKLLSDDGIDFSYNIIGDGPELERLKFAVYQYGLENKI
                     NFCGKKSHDEVLEIMGNSDIYLQPSYEEGFCVSVLEAQAAGLMCIVSDAEGLKENVVD
                     GESGWIIPKRNFKSFNEKIAEVCKMSFVDRKKISDTARERVKKEFRLEDQNEKFKEFF
                     QNMINQNG"
     gene            10836..11759
                     /locus_tag="PLX80_04775"
     CDS             10836..11759
                     /locus_tag="PLX80_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY20144.1"
                     /translation="MGRTISIVSPVYKAGKIVDELVRRVSEEASKISDSHEIILVEDC
                     GGDDSWEKIEENCRINPNVKGIKLSRNFGQHYAITAGLKAATGDIVIIMDCDLQDDPS
                     YFKELLKKFDEGYEVVFTKRIGRKHSFFKKVTAKIYNSLFRLFADKNFDLQVGSMTLI
                     SKRVKDEFVKLKDQDRLYIQLLKWVGFKSTYLSVEHKERFEGNSSYTLSKLFFTAVQG
                     WTSHSDKLLRLSIYGGLLISFVTLLIGIYIVITYFMKSFQAGWPSLIVAVLFSTGLIL
                     MSIGITGIYIGKIFEQTKGRPLYIIDKKINL"
     gene            11767..12471
                     /locus_tag="PLX80_04780"
     CDS             11767..12471
                     /locus_tag="PLX80_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017036824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HQY20145.1"
                     /translation="MKKVVITQSNYIPWKGFFDSVNLADEFVFYDDMQFTRRDWRNRN
                     QIKTKDGVKWLTVPVEVKGKYFQKIKDTTISDPGWAKNHWDTITHNYSKAEYFKEYKD
                     FFQELYMGKEYVLISDVNTTFTKAICKLLGIETKIRQSDEFELLEEKSERLVDICKKL
                     NATDYYSGPAAKAYMDESKFENENIKIHYFDYSGYPEYRQLHGEFTHAVSIIDLIFNE
                     GPDAKNFMKTFNKSEK"
     gene            12526..13194
                     /locus_tag="PLX80_04785"
     CDS             12526..13194
                     /locus_tag="PLX80_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008803229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HQY20146.1"
                     /translation="MKNKKLIIIGNTSNARLAKYFFDIDSDYEPIAFSVDKKYITESE
                     FERLPVTAFEDLEKEYPPTEYEAFVAVGYTNMNKIREDLYNKCKEKGYRMANYISTRC
                     SFLTQFPTGDNCFILEDNTIQPFVKIGNNVVLWSGNHIGHDVTIEDHNFITSHVVVSG
                     FVTIKRNCFIGVNATIRDDITIAPETLIAAGAIIMKDTEEKGVYLPARSVLFEKKSDE
                     IKIS"
     gene            13449..14381
                     /locus_tag="PLX80_04790"
     CDS             13449..14381
                     /locus_tag="PLX80_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20147.1"
                     /translation="MKKQNWKKLGMIFMPDRTIEWMQTHAMMPVLDKIENGKARIYFS
                     ARDEKGRSQGAYIKIDLNDPFKIIEISRDPVLKLGQLGAFDDAGIMPTSCVTFENKKY
                     LYYNGWTLGKNVPFFSFNGIAVSEDGGEKFEKLSRGPAVLFSNDVDPYSTFAPYVMID
                     DGKWKMWYVSLIKWTEEKGTLKHFYNIRYAESKNGIDWKREGHVCIDFKNDFEYAIAR
                     PFVLKEDDTYKMWYSYRESEDIKSYRIGYAESSDGMNWERMDECIELDVSENGWDSEM
                     IEYSFIYDFNGSRYMLYNGNSFGKTGAGLAVLDE"
     gene            14408..15556
                     /gene="rffA"
                     /locus_tag="PLX80_04795"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             14408..15556
                     /gene="rffA"
                     /locus_tag="PLX80_04795"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQY20148.1"
                     /translation="MDKERRHIGFNKPYLTGKETEYILDSVRSRKISGDGLFTKKCHE
                     YFENRFGFKKVLLTTSCTDALEMAAILIDTKEGDEIIVPAYTFVSSANAFVLRGAKIK
                     FCDSEAETPNIDVKEIKKLITSKTKAVIVVHYAGIAVDMDPLIELSDKHNFFIIEDAA
                     HSIDSYYKGKPLGSIGHLAAFSFHETKNVVCGEGGMLVINDERFFERSEIIREKGTNR
                     SAFFRGEVDKYGWVDIGSSFLPSDILAAFLYAQLENLDDIQKKRKYIWDYYFQKLMPL
                     KEKGFFKYSIIPEYATNNAHMFFIECRDIEERSRLISYLKEKGIYAVFHYLSLNESPY
                     YKEKCDQIKMENSDRYTDCLLRLPMYYELSDEDLEYVTYYISKFFEEK"
     gene            15565..16995
                     /locus_tag="PLX80_04800"
     CDS             15565..16995
                     /locus_tag="PLX80_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20149.1"
                     /translation="MDKLFRIYNSTGRIGNIILLALLILSGIANRMFMSIKYREIDYI
                     IESVLIVLSLILVRKLFKRSTQVFEKRKLKLFRIILYTGIALRILFAGHDMINRPVQD
                     SDYEKHEKLGIRILEEGEFYDFAGVELRNFRQPGLPMMFAAGLKIYNAPITFSIIMIL
                     FSFGVLVGGYYLFRDLNNIASILAFAYLSISPNMLFMASNSNTQLSFFFFLIILFIIL
                     KNFNGKTYQLLIIGALLAAEMYVRFNFIMIFLLIPFFIEFYKDKKLSFALGKYAIVFA
                     GFLLLYSPWIIRNYMTYGTIRLMPTTGLGLYSTNMTNDPKKVGDYNGVPDSVLKKYSK
                     LSEVEFDEALKKETFDFVKENPDVIIKSMPFKMMKYSGRQDWTISYFFQFTEYEGDPF
                     MEGLFQSIENFLIWMILFYSIFYLWKNKNLGSLSVYILWSYLVYTMILMPVTETRSRY
                     NFPYILFPVIAISLSNGNNIRKEIPD"
     gene            16995..17867
                     /locus_tag="PLX80_04805"
     CDS             16995..17867
                     /locus_tag="PLX80_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQY20150.1"
                     /translation="MPGKLLIIWEFESALGKINSNSPDNFSYDPIYQEIENVDYILTC
                     AESENIQMTFACLGFAAIQGVFPFNIPDIISKLSAKGHEIASLSWRNEWFPNLTEKQI
                     HSSLERSKKSLETCLGIEDKVTGFVFPHNRPMSWIKKKYFNFEDRGLYPFHKGSDIGF
                     VLKQMDSLDYKWCKVLTKYKTFREKIWGNSDEEIMSFPKWEIHENIVCVPEHYTGFDK
                     TAIDFIKEAVNRNETAVICGHPANLSKDKNESRDHFDRFIDKAVSFREDDGLEIQTVS
                     NFIRKYNPVTENKY"
     gene            17972..19342
                     /locus_tag="PLX80_04810"
     CDS             17972..19342
                     /locus_tag="PLX80_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQY20151.1"
                     /translation="MKAYRNFKIIEYLNPGWYFNLIPVDSLSYSICCFNSDVMKFPDE
                     FERIIFPDSRFETKTAIDLDAGFQAWNRGIMLTAKNIPLDKIQHKENISVKDEYIFIR
                     KYYKWHRVYYYLIKNLLSFNNPFKEIGAFISTRDIERTELINPHLEYEGYKSFESEIL
                     KNNPKVSVIIPTLNRYKYLKDVLEDLEKQDYNNFEVIIADQTDVPNIEFYDKFNLNLK
                     IIFQDGKGQWLARNEAARKCKGDYFLFFDDDSRVDKDWISEHLKGLDYFKADISAGVS
                     LSTVGDAIPENYSFFRWADQFDSGNAMVKREVFEKTGMFDRQFDKMRMGDGEFGLRAY
                     LAGFKSISHPFAKRIHLKVSSGGLRQMGSWDAFRPKNFFSPRPVPSVLYLYRKYYPSD
                     FVSNAMIIGLLPSLIPYKWKGKKYLYPLGALAAIFILPVLLIQLGISWKRSSEMLKEG
                     DKIEWL"
     gene            19329..20462
                     /locus_tag="PLX80_04815"
     CDS             19329..20462
                     /locus_tag="PLX80_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20152.1"
                     /translation="MSGFKNYLMLTTEFPPGPGGIGNHSYNLAKYLSINEIPVTVLAV
                     SDFANEYEKNEFDKYQNFKIKRFERYSNRLKTYRERISLIKKTIRDEKFSHIIFSGRS
                     SLMAMLLLKGLKGNTKFISIAHGGDINAENKFEKTLVNRALEMSDLIIPVSRFSGSKI
                     DEKIDKKKIKVIPNGFDMDMSAHIEFLKDKRDKITGAVKLISVGTIWPRKGHHNVLES
                     LPEIIEDHPGTTYTIVGRNADTSLCDKYFADEKIKGSINKAGQISNEKMYELLNDSDI
                     FILLSETQSSGDFEGFGIAVIEANHFGLPAIGSFNSGLEDSIKDGVSGILVDPKNKEQ
                     IRKAIDTICNNYESFSRGAKEWAEEHHWTVIIKKYLNAIDNIN"
     gene            20469..>20827
                     /locus_tag="PLX80_04820"
     CDS             20469..>20827
                     /locus_tag="PLX80_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQY20153.1"
                     /translation="MCGFLGAIFFNRTFEQYIPALRKGLKAISHRGPDGSHELTSVNC
                     WLGHNRLSIIDLSKEADMPMSSCNSDVHIIYNGEIYNYSVLKKELECTRFKTRSDSEV
                     VMEGYIKEGTDYFKKLRG"
BASE COUNT         7762 a         2633 c         4027 g         6405 t
ORIGIN      
        1 aaaagcagat cattaccaaa agaaaaagcc aaattaaaaa tagtgaaagg tgacaaccgg
       61 aaatgaagaa aattgcattt gtttcttcta ataaatcaga atggggagga agtgaatttc
      121 tatggtacaa tactgcattg atattttcag aaaagggaaa tgatgtactt gttacaatac
      181 ctcgttggaa aaaaatccct gaacagatag attttcttaa atcaaaaaag ataccgactt
      241 attttaacac agatacatca acatataaaa aattatttaa cagagtactc ccttcttcaa
      301 tgcagtttga ttatagtaat gacgggaata aatgtttaag agagttttct cctgatcttg
      361 ttgtgattaa tcaaggtgga aatacgggag gagcagatct gatggaatat tgcataaata
      421 gtggattaaa gtttgttact atttcacagg cagcaaacga agcaaaatgg ccaagtgaca
      481 gtttaaataa aaggctgtcc gaatatcttc caaaagcaat aagaaattat tatgtaagta
      541 aagcgaatat taagttaact gagttacaga caggtcagaa aattgataat gcagaaatta
      601 tttttaatcc atttaatgta agctataaca atcgaataaa ttatccggaa gtcagagaaa
      661 attattttat agctaatgta gcacgtcatg aattttttgc aaaaggtcag gatatattat
      721 ttcaggtatt gaatgaaaaa aaatggcggg aaagaaattt gattgtaaat ttatttggta
      781 aaggtgaaca ttcgtttcag ataaaaaagc ttaaagatta ttttggatta gataaagtcc
      841 agatcaaagg tcacaaagat cctgaagaga tctggaaaga aaaccatgca atgatactta
      901 cttcaagata tgaaggtctt ccgcttgcat tagtagaagc aatgctttgc gggcgtgtat
      961 caatagtaac tgatgtgagc ggaaacagag aagtactgac agaagggaaa acaggatttc
     1021 ttgccaggca tccggatccg gaatcagtga gtgaggcatt ggatagagca tggcaaagaa
     1081 gatatgaatg gaaagaaatt ggtgaaatag caagagaaga aatcaggaag atagttccgg
     1141 aaaatccttc tatattattt tatgaaaaat taaactccat tgtctgagga aaaattaaaa
     1201 attaaatcag aaactattac tgaaaaattg aaatttccaa atctggaagt aaagtataag
     1261 atctgtatag tatcagcaat actgccgcca gcttatggtg gagcagaagt agctgctttt
     1321 aaatatgctc ttagattaga aaaagatcca gaatccaata tacttgtaat tgggtgggac
     1381 agaacaggtg gatataaaga aagtaaaaat aaatttgaat ttgtagtacc tgtaacattc
     1441 agcgaaaatc ctaaagatgc aaaaggaata ttaatttatt ttcagcagta tcatcacatg
     1501 tggaattgtt ttacgaaact tttgaagcca atgtggaagc accgtaaaga atatgattac
     1561 atacataatt ttaattcagg atttgcattc aatagagtat cgattcttat tgcaaaaata
     1621 ttaggaaaaa aaattgtcac agagacttca ctggtaggtg atgatgatcc tttatctctt
     1681 ggaagatttt tggactggaa ggattatctt aagccaaaat ttatcagata tttattttat
     1741 aagcttgcag acagatatgt aagtaaatca aatgtgatta ctgatatata caggaagtca
     1801 gagatctcta tgaataaagt cgttcagatc ccatattcgg tggatataaa taagttttat
     1861 ccacctgagc ctgaaagaaa aaagaagcta agggaaaaat ttaaaattga tgaaaatgat
     1921 gtcgtagtaa tatttgtagg cggtattaat gtcaggaaag gagttcattt acttgtgaat
     1981 tcatttgtag atgttcataa aaattttcct tcatcaaaat tgcttattgt tggtccaaca
     2041 tataaatata atcagcaata tattattgac ataaagaata agatcagtga aaattccctc
     2101 gaggataaga ttattttaac agaagataat gtaagtaatg tagaagaata tatgcagtgt
     2161 gcagatattt ttgtgctgcc ttctaggcag gaaggatttc caatttcaat tatagaagca
     2221 atgtcatgcg gtttggttgt gattggttcg gacattcctg aaatttcaaa agctcagata
     2281 gtaaacggag tagatggttt tacattttca ttaagtaaac cggaggaact tacttttata
     2341 ttaaagaaat taatgggaaa caaagatgag ttaattaaaa tcggaaatga agccagagct
     2401 aaagttgtta aatactggtc cgatgaaatt gtagataagg aatataagaa attatattca
     2461 tctatcaatt ttgtacttcc aataaataca tataataata ttaacacaaa aaaagaacgt
     2521 gaaaaaatta agatccttta tacaattcca aatttcaata ctgccggtag tggaaaagca
     2581 cttattaatg tgatctcaag attagaccga aacgtatttg aaccttttat ctgctgcagg
     2641 catgacagag gtgatctgtt cagcaaagta caggaacttg atataccggt atacattacg
     2701 gattttacag tatcaacaaa gccaagaata aaaggcttta aaaatgtatt aaagctttca
     2761 aaaaatttta aaaatattga tcctgatata attcattctt acaattattc tgatgattat
     2821 tcggaagcac ttgcttcaaa gctcagcagg atcaaatgga tctatacaaa gaaaaatatg
     2881 ggctggggaa gtaatgcgtg gaaaatcaga tcaaaactgt caagtgcaat catccctcaa
     2941 aatcaggaaa tggtagaaac atttttcaga ggaatcaagg aacagtattt aataccaatt
     3001 ggaattgata ttcaggagtt taaaaaaaca agcagagatg aaagtattat tacaagatat
     3061 aagttataca attcgaatcc aataattatt actctggcaa atgtaatccc gattaaaggg
     3121 atagattatc tgatcaaagg atttttactt gctcttgaag attataaaga tgcaaaactt
     3181 ttaattatag gggatgatgt aacagattat gcaaaggatc taaaaaagaa aactatagac
     3241 gcaggacttg aagggaaagt tttttttaca ggaaaacaga aagacataaa gccttttttt
     3301 agtatagccg atatttttat tttatcttcg aataaaaccg gtgaaggtgg accgatctca
     3361 atccttgaag caatggcaag cgggataatt tgttacggaa gtgatgtacc aggaatccga
     3421 gaccagttcc gtgaatttga agatcagctt tttgaatcag aaaaccccgg atctattgca
     3481 aataagatca tgaattatat gagaatgaat cctgaagtta agcaagaaaa gataataagc
     3541 cagcttgagt ttgtgaagaa gtattattca atagaaaatg aagtggataa acttcagaag
     3601 ttatatctga aacttattaa aacataagat agctggaaat gaaaggtata aaattcctta
     3661 ggtatctgcc acattatctg agaaaaaaat tcaaagaaaa aataatagta atcgaaagtg
     3721 atgactgggg acttgagaga gcgatgaata tagattctat aaattttctt aaaaaaaaat
     3781 attctgaaga aaaatttaca agatggacat ttgatgcact ggagacaaat gaagatattg
     3841 aattattata tgaagtactt ggaagtttta aaagcaattt tgaataccct cctgttataa
     3901 ctgctaattt tgttacacat aatgtagatt atgattcatc tgatgatctt aaattcattc
     3961 cgataagtaa aggattcaat aaagattctg aagatgtcag agaattatat aaatgtggaa
     4021 ttgagaataa ttttatatat ccgcaactac atggattcag tcattataat ttatcagagc
     4081 tgacaaaata tttttataca aaagaaggac aggaagcatt tgagaatagt ttttttacag
     4141 gatcaactac aataaaaggt aatctgaaat ttttaacagg tgaactttgc ttcatgaata
     4201 aagatactaa gataaaggaa tcaattgatg aactttgtaa aatttttaat gtagaatctg
     4261 aaacgataat accgcctaat tatattcttt ccaaacaaaa tatagatgat ttaaagaata
     4321 atggaatcaa aatgattcaa tcttcaaaca gattagtaca gtcggacgga agtcgataca
     4381 gttttccata ttttagaata agaaacggac ttgtatggtc agtaagaaat gccagacttg
     4441 atccgcatat tgactatgga ttttatcatg acgaatgtat caatgatatt ggcaaagctt
     4501 ttgataataa attacctgcg ataatagatt ttcacagagt aaatttttca ggaaaattta
     4561 ctcctttata cagagacaaa acattgtcag agatgaaaaa attatttgaa tgtgtttata
     4621 aaaaatggcc agatgtaaaa tttttaaatt caagcgactt aaataaatta atatggcaac
     4681 aagaaaccag ataagttttt taaaaaataa tattgatatt atcattgaac ctgttttgat
     4741 agtaggatca aagcactatg aatttgacaa agaagatatc agagaaagac taaaagagat
     4801 gggaaaaacg gatgtaacag gtattgatat ttcagaaggg gagggagatg attacgttgt
     4861 agatattact gacagtgaat gtgaattttt aaaagagaaa tcagctaaat attctactgt
     4921 gatttgtatg gaagttttga ctcatgtaag aaatcctttt aaagctgctg aaaatttatc
     4981 agggttttta aaagaaggag gtactgcgat tctttcagaa tgctatacca gaaaaatctc
     5041 aaaaatgcct attgatctct ggagatttac ttatgatggt acaaaagaat tattcaaaga
     5101 tttgaaattt agtgatgaaa gagccagagt atctctgacc agagagaagg atgaaaagtt
     5161 acttccactg aaatatccat tgcctcaatt gatggcacag aagagcgatg atgaaaatgc
     5221 agcaggttat tttttaagaa gactccatag aaaatatttt gcaaaaggaa tattcagaat
     5281 ttcaaggctg ctaccggaga ctactattta ttcaatcgga aaaaaataaa tttaaaaatt
     5341 taaagttaaa aattgggtaa ggataatatg tatgagaatc taaaagacag agtagctttt
     5401 agtttatcaa aaagaaattt aaaaaagata ttgagttcag aagatattga tgaagtatta
     5461 aaatcaattg agatttacaa gggcagggga ttctatgata aaatcagact tgctcaggag
     5521 cacacagaaa taagagatct ttgtaaaaaa gtagtggaac ataaaccaaa gattataaca
     5581 gagatcggta cttggaacgg aggtacgttt tacgtctggg cgaggacaaa tccgcatgct
     5641 gaaaaaataa tcagcataga tcttccagat ggagattatg gcggaggata tgatgaaaag
     5701 agaattaaat tttttagtga atttgcatct gacagaaaaa atacttcact gtcatttatt
     5761 cgcggagatt cgaaatcaaa tagtactgta gaaatgctga agtcagtact tggagacaag
     5821 acaatagatt ttttatatat tgacggggat catacatatg acggaatcaa aaaagatttt
     5881 gaaatctatt ctgaactaat gtcttctgac gggataatcg gatttcatga tattaataca
     5941 cacaaagatg gttatggggt tattcaattc tggaatgaga tcaagcagaa ttacaggtat
     6001 gaagaattta tcaaacccgg atcaagagta atgggtaatg gattaattta tttaaagaaa
     6061 gattaatttg ccggaagttt caataattac tccgctgtat aacggatcaa atacacttga
     6121 agaaactgct gacagtgtat tagcacagga tttcaaagac tgggaatgga tattttttga
     6181 tgacggaagt acagatgata caattgaaat agcgaaaaaa attcagtcaa agtttccgga
     6241 taaaatattt ttatatactc atgataacaa cagtaatcac ggtactgcat tttcaagaaa
     6301 cagagcattt gaaaaatcca aaagtgacat cattgcattt atagatcagg atgatatctg
     6361 gtatgaaaac agactttcac atcagataaa tatttttaga aataaaactg aatgtgcaat
     6421 gatctgggga cctgcattgt actggtataa ggacagatct tttgtacaat cagttggatt
     6481 aaatggaaaa ggtttgaaaa gcggaattta tgatccacct gattttgtgg aaattttttt
     6541 aagtaacctc aaaggtactc ctcttccaag tgcaagcttg gtcagaagaa aagaatttgt
     6601 aaatgtgaaa ggatatgaag aatcgataaa aggttcagaa gatatagtct tatggctaaa
     6661 gctatccgaa aaattccaga tatattatga tgatgaagtg cttattaaat acagaaagca
     6721 tcaggactca actttacgga aagcaagtga cagtggtaaa atggatgaat ggaatcttgt
     6781 attttataaa tgggtgattg attttttaaa aagaacatcc tcaaacgaaa gtattctcga
     6841 agaaaatgaa tttgcatttt ataaaaatct taaaaaaatt tcaggtaaag aaaattattt
     6901 aaaaagcaga aaagatcttt ataacagact caatgaatat ccggacatat ggaaaaaata
     6961 ttcaggtgat tatttattag atctggtatt gccttttgat acggcatcca gaatttctgc
     7021 aaaactgaga tttgatctgt ttaaaaaaaa ataattgaga actgaaataa ttccttataa
     7081 gcagcagttt tataaaaatg gtaatgaatc cggtttagat ctgaaagcaa tttgtatttt
     7141 ttcagcttta ggtttttttt taggaagaga cacatattac aaaaatcttg aggcgttaca
     7201 gccggcatct gattacaata tagatgaaac cggaaatata atatccgctg aagaatactg
     7261 gaagtggcat tacagtccac gggaaatgaa tcttaaacag gcaacagagg agtttgcaga
     7321 tttatttgaa aaattaattc atgaaaatct taaagataag aaagttataa tgccattatc
     7381 aggaggcatt gacagcagat ctcaggctgc tgcaatcgga aataaatttg atacaaaaag
     7441 ttattcctat aaatttgaaa aaagttttga tgaaacaaaa tacggaagac tgataagtga
     7501 agtacttgga attccatata aagaatatgt gattccggaa ggttatctct gggacgtaat
     7561 agatgaactt gctgaaataa atcaatgcta tgcggacttt acacatccga gacaaatggc
     7621 agtaataaag gaggtctcta aatttggaga tgtatttttt ctcggacact ggggagatgt
     7681 tttatttgat tcaatgggtg tagatgaaga attgagattt gatgaattag taaatacggt
     7741 cttaactaaa attgttaaaa aaggtggaat tgaacttgct gaaatgttgt gggaacattg
     7801 ggaactggaa gggaaattca gaaattatct ggaagacaga atttcagaac ttctaagtga
     7861 aattaaaatt gataatgcca attctagaat cagggctttt aaatccatgt actgggctcc
     7921 gagatggaca agtgcaaaca tgaatgtatt cagtaatttt catccgatat ttctgcctta
     7981 ttatgaaaat gaaatgtgtg aatttatttg taatattcct gaggatttac ttgccgggag
     8041 aaaaattcag atcgaatata ttaaaatgaa aaatcctgaa ttagcgaaga taccatggca
     8101 gtcttacgaa cctttgaatt tatataatta taagaatttt caaaacattg gaaatttacc
     8161 cggccgtgta gtcagaaagg taaacagaat tattaatgag gatgttttaa aaaagaaaaa
     8221 aactaagagt aattatgaga ttcagttttt aggaaaaaat aatgacagtc agttgaaaaa
     8281 gagacttttt gaaaatgatg atctaaaaaa tatcttaccg gaaaaaattg ttaaggatat
     8341 ttacggaaaa tttataaatg aagacagcaa aaaatattct caccctgtga gcatgctcct
     8401 tactatttct cagttttcta attcattatc atttaagcag agagagtcag aatgaaagta
     8461 gcaatttatt ccggcaacat tccttcaact acgttcattg aaaaccttat cagaaattta
     8521 tctaattctg gattcagaat attattattc ggtaaaaaaa taagagatgt aaaatacaac
     8581 gaaaatgttg aagtgcataa tactcctaat aatagtatta tcatattatt ttttattttg
     8641 aaagaatcct tgaaattatt tttcaaaagt ccgaataaat tttcaaagct tttgaaatat
     8701 atttcttcat cagacacagg tctgaaaagc aaactcaaag aacttggaat gatgctgcca
     8761 ataataaata atgaaccgga tatatttcac atccagtggg caaagacagt tcagaaaaat
     8821 tcatatttat ttaatctaat gaaatgcagg tttgcattga gtctgagagg tgctcatatt
     8881 aattattcac ctcttaatga cgaatcgctt gctgaaagtt accgaaaata ttttcctaaa
     8941 ttagatggtt ttcatgcagt atctaaagca atatcaattg aagcgcagaa atatggagct
     9001 gctcctgaaa aaatcaaagt gatctattcg agtgttcaag atgagcttct tcaaattaaa
     9061 acggaatcaa aaataaatag atcagaagta actaatataa tttctatagg aaggtatcac
     9121 tggaagaagg gttatcatta tgcatttgat gcgatgaaga ttctgatcgg aaagggattt
     9181 aattttcaat atacaatcgt agcacaaggt gatgttcccg aagaaatatt atatatactt
     9241 tctgataaag aattaaaaag cagagtagtt ttaaaaaatg gtatggctca tgatgaatta
     9301 atgaatgagc ttaaaaaatc ggatatgctt ttacttccaa gtgtagaaga aggtattgca
     9361 aatgtagtac ttgaatcaat ggcgatcgga gtaccggtta taagtactga ctgcggcgga
     9421 atgaatgaag ctgtttcaga ttttgaaaat gggatgatgg caaaagtaag agatccgaat
     9481 atgcttgctc aaaaaattat cgagtattct gaattaagtg agaataaaaa ggaacagatg
     9541 aagctcattg ctaaaaatac tatttcagaa aagtttacag ataaaaaaca aatagatggt
     9601 tttgtgaatt tttataattc cctgctatat agctagggtt atgaaaatag gattggtatt
     9661 acctgaagtt ccgcaatatt cagaaacatt ttttaatttt aaaataaacg gactggtaga
     9721 atcgggattt gaagtattac ttttcacagg taataaaaaa aattcaaaga atagagctaa
     9781 cgaaaaagta aaagtaatac ctgcttatcc ggtatataga agcagcagta tctatcagtt
     9841 tttattattt atatatgtgg tatcattcag atttattttc tcactaaaga aaacattaaa
     9901 actaattagt ctggagtcaa aggttgggaa aaatttttca gaaagaatga aatcagtata
     9961 tctgaatgca catatcttaa aagagaatat tgatgtgctg cattttggat ttacgactat
    10021 ggcaatacag agagaaaatg ttgcaaaggc aatgaatgca aagatgagtg tgagtttcag
    10081 aggttatgat ataaacattt atccattgaa aaatcctgac tgttataaga atgtctggag
    10141 gaacatcgac aaagtccata caatatcaaa ttaccttagg gagaaagctg tatcgcttgg
    10201 tcttccggaa aaaactcctt acataaagat cacgcctgcg atcgactttg aaaaattaag
    10261 tcagaataaa atgagaacgg atatttctgt tccaaaaata ttaacagtgg gcagattaaa
    10321 ctggataaaa aattatgaga cagctatatc tgccttgaaa ttactgtctg atgatggaat
    10381 agatttttcc tataatatta ttggagacgg tcctgaatta gagcggttaa aatttgcagt
    10441 ttatcaatat ggtcttgaga ataaaataaa tttttgcgga aagaaatcac atgatgaagt
    10501 attggaaata atgggcaatt cagatattta tttgcagccg agttatgaag aaggattttg
    10561 tgtatcggta ttagaagcac aggcagctgg tttgatgtgt atagtcagtg acgctgaagg
    10621 gctgaaagaa aatgttgttg acggtgaatc aggatggatt attcctaaaa ggaattttaa
    10681 atcatttaat gaaaagatag cagaagtatg taaaatgagt tttgtagata gaaagaaaat
    10741 ttcagataca gctagagaaa gggtaaaaaa ggaatttcga ttagaagatc aaaatgaaaa
    10801 gtttaaagag ttttttcaaa atatgataaa tcaaaatggg tagaaccata tcgatagtca
    10861 gcccagttta taaagcggga aagatagttg atgagctggt aagacgtgta tctgaggagg
    10921 catcaaaaat atccgacagc catgagatca tattagtgga agactgcggc ggtgatgatt
    10981 cctgggaaaa gattgaagag aactgcagaa taaatccgaa tgtaaaaggc attaagctca
    11041 gcagaaattt cggacagcat tatgcaatca ctgcgggttt gaaggctgct acaggtgaca
    11101 ttgtgataat aatggattgt gacttacagg atgacccatc ttattttaaa gaattactta
    11161 aaaaatttga tgaaggatat gaggtagttt ttacaaaacg aattggaagg aaacattcat
    11221 tctttaaaaa agtaacggca aaaatttata attcattatt cagattattt gcagataaga
    11281 attttgatct gcaagtagga tcgatgactt tgattagtaa aagggtaaaa gatgaattcg
    11341 taaaactaaa agatcaggac agattatata ttcaacttct taaatgggtt ggatttaaat
    11401 caacatattt atcagtcgag cataaagaaa ggtttgaagg caattcatct tatacattat
    11461 caaaattatt tttcacagca gttcagggct ggacatcaca ttcagataaa cttctgaggc
    11521 tgtctatcta tggcggatta ttgatctcat ttgtaacttt gctcattgga atatacatag
    11581 taataactta tttcatgaaa agttttcagg caggctggcc aagtttgata gtggcagtac
    11641 ttttttctac cggtctgatt ttaatgagca taggaataac aggaatttac attgggaaaa
    11701 tatttgagca gaccaaagga agacctcttt atataattga taagaaaata aatttataat
    11761 atagttttga aaaaagttgt aatcacacaa tcaaattata ttccctggaa aggatttttt
    11821 gattcagtaa atcttgcaga tgaatttgtg ttttatgatg acatgcaatt tacgagaagg
    11881 gactggagaa acagaaatca gataaagaca aaggatggag taaagtggct tactgtgcct
    11941 gtcgaagtaa aaggaaaata ttttcaaaaa attaaagata caacgatcag tgatccgggc
    12001 tgggctaaaa atcactggga cacgatcact cataattatt cgaaggctga atatttcaaa
    12061 gagtataagg atttttttca ggagttatat atgggaaagg aatatgttct gataagtgat
    12121 gtgaatacta cttttacaaa agctatttgt aaacttctcg gcattgaaac taagatcagg
    12181 cagtcggatg aatttgaact gcttgaagaa aagtcagaaa gacttgtaga tatttgtaaa
    12241 aaactcaacg ctacagatta ttattcagga ccagcagcaa aagcatacat ggatgaaagt
    12301 aaatttgaaa atgaaaatat taaaatacac tattttgatt acagcggata tcctgagtac
    12361 aggcaacttc atggagagtt tacacatgcg gtcagtatta tagatctgat ctttaatgaa
    12421 ggtcctgatg cgaaaaattt tatgaaaaca tttaataaaa gtgaaaagtg aaaagtgaaa
    12481 aattttatga aaacttttaa taaaatgaaa aatgaaaaat gaaaaatgaa aaataaaaaa
    12541 ttgattatta tcggcaacac atcaaatgcc agacttgcaa aatatttttt tgatattgat
    12601 tcggattatg agccaattgc attttcagtt gacaaaaaat atataaccga atccgaattt
    12661 gaaagacttc cggtaactgc atttgaagat ttggaaaaag aatatccacc tacagaatat
    12721 gaagcatttg ttgcagtggg ttatactaac atgaataaga tcagggaaga tctttataat
    12781 aagtgtaaag aaaagggata ccgaatggca aattatataa gtacccgatg ttcattttta
    12841 acacagtttc caacaggtga caattgtttt atacttgaag ataataccat acaaccattt
    12901 gtaaaaatag gaaacaatgt agtgttgtgg agcggcaatc atattggaca tgatgtaaca
    12961 attgaagatc ataattttat aacatcgcat gttgtagttt caggatttgt aacaataaag
    13021 agaaattgtt ttataggagt aaatgcaact atacgtgacg atatcacaat agctcctgaa
    13081 acactgatag cagcgggtgc aataattatg aaagacactg aagagaaagg tgtatatctt
    13141 cctgcaagat cagtgttgtt tgagaaaaaa agtgatgaga taaagatatc gtaattttta
    13201 ataaggaaat aattagaatt taaattgact cttttacttc aacaagattc tttgaaattc
    13261 attgaatcat ttttcatccg gcaacaactc actttgctcc cctttttaaa ggtggaatta
    13321 cttttgaatt attctgaaag gtgcttttaa aactttagat tttctgtctg ataaattcac
    13381 tgaaaaattt cttaaaattt atgtgacgaa tttgagtttt acattaataa ttagataata
    13441 aattttattt gaaaaaacag aactggaaaa aactgggaat gatctttatg ccggatagga
    13501 caattgagtg gatgcagact catgcaatga tgcctgtatt agataagatt gaaaatggaa
    13561 aagcccgtat ctatttttca gcaagagatg aaaaaggcag atcacaaggt gcatatataa
    13621 agatcgatct gaatgatcct tttaaaataa ttgaaataag tagagatccg gtattgaaac
    13681 ttggtcagct tggagcattt gatgatgcgg gtatcatgcc gacatcctgt gtgacatttg
    13741 aaaataaaaa gtatttgtat tataacggtt ggactttagg aaaaaatgtc ccattctttt
    13801 cttttaacgg aattgcggta agtgaggacg gaggtgagaa atttgaaaaa ttgtcgagag
    13861 gtcctgctgt tcttttttca aatgacgttg atccttattc gaccttcgct ccatatgtga
    13921 tgatcgatga tgggaaatgg aagatgtggt atgtctcttt gataaaatgg acagaggaaa
    13981 aaggaactct gaaacatttt tataatatca gatatgcaga atcaaaaaac ggaatagatt
    14041 ggaaaagaga aggtcatgta tgtatagatt ttaaaaatga ttttgaatat gctatcgcaa
    14101 gaccatttgt attgaaagaa gatgatacat ataagatgtg gtattcttac cgggaatccg
    14161 aagatataaa atcttataga atcggatatg cggaatcttc agacggtatg aactgggaaa
    14221 gaatggatga atgtatagaa cttgatgtat ctgaaaatgg atgggattct gaaatgatag
    14281 aatattcatt tatatatgat tttaatggaa gcagatatat gctttataat ggaaatagtt
    14341 ttgggaagac aggtgctgga cttgcagtac tcgatgaata atatcaattt aattttataa
    14401 aaatttattg gataaagaaa gaagacatat aggatttaat aaaccatacc ttacgggtaa
    14461 agagacagag tatatattgg attcagtcag atcaaggaaa atttcaggag atggtctttt
    14521 tacaaaaaaa tgtcatgaat attttgaaaa caggtttgga tttaagaaag tacttttaac
    14581 gacttcatgt actgatgcac ttgagatggc agctatattg attgacacaa aagagggtga
    14641 tgaaattatt gtgccggcat atacttttgt atcatctgca aacgcgtttg tattgagagg
    14701 tgctaaaata aaattttgtg acagtgaagc agagacacca aacatagatg tgaaagaaat
    14761 taaaaaacta ataacttcta aaaccaaagc agttatagtt gtacattatg caggaatagc
    14821 agttgatatg gatccattga tagaactttc tgacaaacat aattttttta ttatagaaga
    14881 tgcagcacat tctatagact cttattataa gggtaaaccg cttggttcta tcggacatct
    14941 cgcagcgttt tcatttcacg aaactaagaa tgtagtttgc ggtgaaggcg gtatgcttgt
    15001 aataaatgat gaaagatttt ttgagcgttc agaaataatc agagaaaagg gaactaacag
    15061 gtcagcattt ttcagagggg aagtggataa gtacggatgg gttgatatcg gatcttcatt
    15121 tttaccttca gatatacttg cagcatttct ttatgctcag cttgaaaatc ttgatgacat
    15181 tcagaaaaaa agaaaataca tctgggatta ttatttccaa aaactgatgc cattaaaaga
    15241 aaaaggattt ttcaaatatt caattattcc tgaatatgct accaataatg ctcacatgtt
    15301 ttttatagaa tgcagggata ttgaagagag aagccggctg atttcttatt taaaagaaaa
    15361 aggtatatat gcggtttttc attatctttc gttaaatgaa agtccttatt ataaagaaaa
    15421 atgtgatcag ataaaaatgg aaaattctga caggtatacg gactgtctat tgagactgcc
    15481 tatgtattat gaattatctg acgaagatct tgagtatgtg acttattata tttctaaatt
    15541 ttttgaagag aagtagagaa ttggatggat aaattattta gaatatataa ttcaacagga
    15601 aggataggaa atataatctt acttgcattg cttattttat caggcattgc aaacaggatg
    15661 tttatgtcta taaagtaccg tgagatcgat tatatcattg agtcagtcct tatagtttta
    15721 tcattaattc tcgttagaaa attatttaaa agaagtacac aggtttttga aaaaagaaag
    15781 ttgaaattat tccggataat tttatacact gggattgcat tgagaatttt gtttgccgga
    15841 catgatatga taaacagacc tgttcaggac agtgattatg aaaagcatga gaagcttgga
    15901 attaggatat tggaagaagg tgagttctat gattttgccg gagtagaatt aagaaatttc
    15961 agacaacccg gacttccgat gatgtttgct gccggattaa aaatttacaa tgccccgatt
    16021 acattttcaa ttataatgat tctgttttca tttggtgtac tggttggagg gtactattta
    16081 ttcagagatc tgaataacat cgcatcgata cttgcttttg cttatctaag catttcaccg
    16141 aacatgcttt tcatggcttc gaattccaat acacagcttt catttttttt cttcctcata
    16201 atacttttta ttatacttaa aaattttaat ggaaagacat atcagctgtt gatcattggt
    16261 gcattgcttg ctgctgagat gtatgtacga tttaatttta ttatgatctt tttacttata
    16321 cctttcttta tagaatttta taaggataaa aaactttcat ttgctcttgg aaaatatgca
    16381 attgtatttg ccggattcct tttgctttat tccccatgga tcatcagaaa ttatatgaca
    16441 tacggaacga taagactgat gccaactaca gggcttggac tttacagtac taacatgact
    16501 aatgacccta agaaagttgg tgattacaat ggagtgccag attcagtttt aaaaaaatat
    16561 agtaagctca gtgaagttga gtttgatgaa gctttgaaaa aagaaacttt tgattttgtt
    16621 aaagaaaatc ctgatgtaat aataaagagt atgcctttta aaatgatgaa atattcgggc
    16681 aggcaggact ggactattag ttattttttt caatttacag agtatgaagg tgatccattc
    16741 atggaagggt tgtttcagtc cattgaaaat tttctgatct ggatgatact cttttattcg
    16801 atattttatt tatggaaaaa taaaaactta ggttcattat cggtttatat actttggtca
    16861 tatttggttt atacaatgat tcttatgccg gtcacagaaa caagatcacg gtataatttt
    16921 ccttacattt tattcccagt gattgcgatc tctctttcga atggtaataa tataagaaaa
    16981 gaaattccag actgatgccc ggaaagctgc tgatcatctg ggaattcgaa tctgcactgg
    17041 gcaagatcaa ttcaaattca cctgataatt ttagttacga tcctatttat caggagatag
    17101 aaaatgtaga ttatatatta acctgcgctg aatctgaaaa tattcaaatg acttttgcat
    17161 gtcttggatt tgcagctata cagggagttt tcccttttaa tattccggat ataatcagta
    17221 agctttctgc taagggacat gagattgctt ctttgtcatg gcggaatgaa tggtttccaa
    17281 atcttacaga gaagcagatc catagttcac ttgaacgcag caagaaatca ttagagactt
    17341 gtctgggaat tgaagataaa gttacaggat ttgtttttcc acacaacaga ccaatgagct
    17401 ggataaaaaa aaaatatttt aattttgaag acagagggtt atatcctttt cataaaggca
    17461 gcgatattgg atttgtatta aaacagatgg atagtttaga ttataaatgg tgcaaggttt
    17521 tgacaaagta taaaactttc cgggaaaaaa tctggggaaa ttctgatgaa gagatcatga
    17581 gttttccaaa atgggaaatt catgaaaaca tagtatgtgt accagagcat tatacaggtt
    17641 ttgataagac ggcaattgat tttataaaag aagcagttaa tcgaaatgaa acagcagtga
    17701 tatgcggtca tccagctaat cttagtaaag ataagaatga aagccgtgat cactttgaca
    17761 gatttattga taaggcagtt tcatttagag aagatgatgg acttgaaatt caaactgtat
    17821 caaattttat tcgcaaatat aatcccgtaa ctgaaaataa atattgaaaa agaaaaaaat
    17881 atttacagtg atttttactt atcaggatgt ttcaaattta tttacaaata ttaagttata
    17941 aagtttttga aattaaagtt cagcaaatag tttgaaagca tatagaaatt ttaaaattat
    18001 agagtatcta aacccgggat ggtattttaa tctgataccg gtggatagtt tatcttattc
    18061 gatatgctgt ttcaattctg atgtgatgaa atttccggat gagtttgaga gaataatatt
    18121 tcctgacagc agatttgaaa cgaaaactgc aatagatctg gatgcgggat ttcaggcatg
    18181 gaacagaggt ataatgctga cagcaaaaaa tattccttta gataagatcc agcataagga
    18241 aaatatttct gtaaaagatg aatatatatt tatcagaaaa tattacaaat ggcacagggt
    18301 atattattat ctgataaaaa acctgttaag ttttaataat ccatttaaag agattggtgc
    18361 atttataagt acgcgagata ttgagaggac tgaacttatc aatcctcatt tagagtacga
    18421 aggttataaa agttttgaat cggaaattct taaaaataat cctaaagtat cggtaatcat
    18481 tccgacactc aatagatata agtatttgaa agatgtatta gaagatcttg aaaaacagga
    18541 ttataataat tttgaagtaa taattgcaga tcagacagat gtaccgaata tagaatttta
    18601 tgataaattt aatttaaatc taaaaataat ttttcaggat ggaaaaggtc agtggctggc
    18661 aaggaatgaa gccgcaagaa aatgcaaagg agattatttt ttattttttg atgatgactc
    18721 tagagttgat aaagactgga tcagtgagca tctgaaagga ttagattatt ttaaagctga
    18781 tatatctgca ggtgtttctc tttctacagt tggtgatgca attcctgaaa actacagttt
    18841 ctttaggtgg gctgatcagt ttgattccgg aaatgcaatg gtgaaaagag aagtatttga
    18901 aaagacagga atgtttgaca gacagtttga caaaatgaga atgggtgatg gagagtttgg
    18961 tctgcgtgca tatcttgcag gatttaaaag tatttcacat ccgtttgcaa aaagaattca
    19021 tctcaaagta agctcaggag gattaagaca aatgggttcc tgggatgctt ttcgtccgaa
    19081 aaattttttt tcaccgagac ctgtacctag tgttttatat ctttacagaa aatattatcc
    19141 atcggatttt gtatcaaatg caatgatcat tggtttgctt ccttcactta ttccttataa
    19201 atggaaagga aaaaaatatc tgtatccgct gggtgcactg gcagctatat ttatactgcc
    19261 ggtattattg atacagcttg gtatatcatg gaagcgttca tcggaaatgt taaaggaagg
    19321 tgacaagatt gagtggcttt aaaaattatc tgatgctcac tacggaattt cctccgggtc
    19381 caggtggaat tggaaatcat tcatacaatc ttgcaaaata tttatctata aatgaaatac
    19441 ctgttacagt cttggctgta tctgattttg caaatgagta tgaaaaaaat gaatttgaca
    19501 agtatcagaa ttttaaaatt aaaaggtttg aaagatatag caacaggctg aagacttaca
    19561 gagaaagaat atctttgata aaaaaaacga tcagggatga aaaattttct cacataattt
    19621 tttcaggaag gtcatcatta atggcaatgc ttttgcttaa aggattgaaa gggaatacga
    19681 aatttatttc aattgctcac ggcggagata ttaatgcgga aaataaattt gaaaaaacat
    19741 tggtgaacag agcattggaa atgtctgatc ttataatacc ggtatctcgt ttttccggat
    19801 ctaaaattga tgagaaaata gataaaaaga aaattaaagt aataccaaat ggttttgata
    19861 tggatatgtc tgctcacata gaatttttga aagataagag agacaagatt acaggagcag
    19921 taaaattaat aagtgtcggg acaatctggc cgcggaaagg tcatcataat gtattagaat
    19981 ctttacctga gatcattgaa gatcatccag gaaccactta tacaattgtc ggcagaaatg
    20041 ctgacacatc tttatgtgat aaatattttg ctgatgaaaa aatcaaaggt tcaattaata
    20101 aagcagggca gatatctaat gaaaaaatgt atgaattatt aaatgattcg gatattttta
    20161 ttctcttaag tgaaacacaa tccagcggag actttgaggg atttggaatt gcagtgatcg
    20221 aagctaatca ttttggtctg ccggcaattg gatcatttaa cagcggttta gaagattcaa
    20281 taaaagacgg ggtaagcgga attttagttg accccaaaaa taaagaacag atcagaaaag
    20341 cgatagatac tatatgtaat aattacgaaa gcttttcacg gggtgcaaaa gaatgggcag
    20401 aagaacatca ctggacagtc attataaaaa aatatttaaa tgcgatcgat aacattaatt
    20461 gattttgaat gtgcggtttt ttaggagcaa tcttttttaa cagaactttt gaacagtata
    20521 taccagcact tcggaaaggt ttgaaagcaa tttcacacag aggacctgac ggaagtcatg
    20581 agctgacttc agttaattgc tggcttggac ataacagact cagtatcata gatctttcga
    20641 aagaagctga tatgcctatg agttcgtgca attcggatgt acatattata tataatggtg
    20701 agatctataa ttattcagta ttaaaaaaag aacttgaatg tacgagattt aaaactcgtt
    20761 cagattctga agttgtaatg gaaggatata taaaagaggg aacagattat tttaaaaagc
    20821 tcagagg
//
LOCUS       DAORVQ010000036        20430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1026_length_20430_cov_5.950626, whole genome shotgun sequence.
ACCESSION   DAORVQ010000036 DAORVQ010000000
VERSION     DAORVQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 20430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20430
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_20430_cov_5.950626"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..2310)
                     /locus_tag="PLX80_04825"
     CDS             complement(379..2310)
                     /locus_tag="PLX80_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQY20154.1"
                     /translation="MKTVIILLMLQISVLTFGQSEKIGDELYLYSDKNSSQPNCTAEE
                     IKFDPAIHENISGPLNMGGPSYDWPLKAALNEGLVIVNYVDDLAGGSIKDYNDNDWSY
                     NGHNGTDICLHDFRNMDRFYAIKAAESGVVIQTVMNNFDRNTGWSNVDPANKILIKHD
                     DGSYAYYYHLMKKSVTVKLGEYVQKGRIIGYVGSSGVSTDAHLHFESGYFVNGTWNKR
                     DPWQGDYNHLPSQWADQEAYVGNTAFKLHDMGVFTSSLIGGDMDNIPISYFKERIFTP
                     NTVSGYEGRIGFWLQLQGNNTGKQIRYEIRRSDGSLFDDTYFYLNSQAQFAWSWWTPT
                     FNVGIGTTGDWYVRVLYDNVEKGRYFFDVQLLTSNRPRMYPVAAKCFRRSIFVQRDTL
                     RVRPVRANMQYQLVNAPPNVSLTNDSILTISTFDQPFRSYDFKVIGSMGGSASLRDTM
                     LYKLIDTTKNHSSGNGIVSLELKSYLEGRYNGSSMEEDTITVLLRSPLSPYALVDYDK
                     VLTLSNGTCIANFPNASSGIYYYILIRHRNSIETWAKTVQQFQLGYPLSYNFTTSKTK
                     AYGDNLKLKGGEYCIYSGDVNQDGAVDVTDVSMVDNDISIFAQGYEVTDLDGDNFVDA
                     SDQAIADNNSFNFVVKIRP"
     gene            2662..2748
                     /locus_tag="PLX80_04830"
     tRNA            2662..2748
                     /locus_tag="PLX80_04830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2696..2698,aa:Ser,seq:gga)
     gene            2963..3049
                     /locus_tag="PLX80_04835"
     tRNA            2963..3049
                     /locus_tag="PLX80_04835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2997..2999,aa:Ser,seq:cga)
     gene            3450..3722
                     /locus_tag="PLX80_04840"
     CDS             3450..3722
                     /locus_tag="PLX80_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY20155.1"
                     /translation="MTKEELVTKMAGDAEITKVQARAALESIIDGVKKTLKKGGKLSL
                     VGFGTFSISKRKARLGRNPQTGKEIKIAAKKVAKFKAGKTLADHVK"
     gene            complement(3786..8114)
                     /locus_tag="PLX80_04845"
     CDS             complement(3786..8114)
                     /locus_tag="PLX80_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY20156.1"
                     /translation="MNSVKILTTLFVLAILFCSHTVFSQTTVIIKFKENTPASVISNF
                     KNNRPESGNSTVSKLSRDFGLKKSKPVFNERVLQNFDNRNLSDAISKTGLNRIFISET
                     NNENLDLLLTAAGKNEFIEYIQFNNVLKVDDIQGPPVTPNDPYYSDQNYLNMIGMNNA
                     WDITLGDSNIVIGVIDTGLDFLHPDLQNSFKINYGEYGNGKESNGIDDDNNGFIDDWR
                     GWDFTDEPLTGDPRRGDYLDPDNDPTDDNSVSHGTAVTGIINAGFNNITGISSVAPGC
                     KVMVLRAFDAEGYGEEDDVANAILYGVANGVKVFNFSFGDYVFSNLLKDVIRFAYLQN
                     VLIICSAGNDGTDRLHYPSAYDEVVSVGASDNSNNKASFSSYGETVDIFAPGFQNLTT
                     VRVGKGSSQYGGDYNKLNGTSFAAPIVAGISGMLLSKNPSLTNEEIRGILVSTTAMLQ
                     GQNSWDHVRSSGRVDALNALQNFNNPTISRILTPSQDYTFENDTVTVSVSAASNLFLS
                     YSLYYAKGQRPSEWIPLLSNQTSQVLGDTVLKWNTAGLQDTSYTLRLAINSNSGRTIE
                     HRMIIFKDKNPPMFTDIQFGNLIDKNNFSQLILFATNKRTLGKIYYKRKGSSEPYISV
                     LADIGTPNIGFVTNAHFGLLNGNNLAPNTEYEFYVEAISLNGKSATFSDTAFYFYTQP
                     SINNYGFNNKNYKLGNSQFARTVTDINGNGLNDLLVNDINNNLKLNVYEFSNGNFQKI
                     SNDNWGDFKIARDIADVDGDGKLEILASSDRNGFLYEAPNQNAIPTDLVWSDQGNGNF
                     WSSMIADTDSDGKNELLGFGNSGLRILESTSGNNFNQTANLNFYGTDSITNSQVVLVE
                     DFDNDGKKDIMFSNIFYSPSNPGIPITCVSVYENVSDNNYQRVFIDSIYRFIKGENIV
                     SGDFNGDGKKDFALGVTSQNSDFIQYYSLYSYTSAGDNQYELLDITDIYNYKDYTKTS
                     TLSADIDNDNRDEVIVNVGTLFYILKFNTTLQRFMPDYYREGMNSFNQIAFDFDGDGV
                     KEIGANTVDDSLIFFEKDIAFTGPATPLNLKGHSIDSNSVELTFEPVSGAERYKVYKS
                     DSTMNFSLYDSVVTNLFIDLIILNRKNYYYKISSVDEQNSVKESMLTDPVKVYVHNKS
                     QLLSAEYQNGGFLVLKYSERIPTLIPNLSSFVISGNSAVPFNPKNITAKSNYEYFLTM
                     NSIPENGNYNVKALVLTDYYGSPVDTNTKSFTVLETDSIKFYLSKLELIGEYRLTAEF
                     NMEVDSVSGRNTSNYKFQPLNISVSSVTIDNADRKKVYIDLEKNSVIGATGKNYIMNV
                     KNVYSSGGIKIVDGAGSSLGLIFNKENLNEMYVYPNPYSVSTGQDYITFANITRNASI
                     DIYDLKGKFILTIAEADGNGGVEWDLMDSGGNAVPTGIYIYVASGKNSDGQDVEEFTG
                     KFAVVK"
     gene            complement(8158..9936)
                     /locus_tag="PLX80_04850"
     CDS             complement(8158..9936)
                     /locus_tag="PLX80_04850"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQY20157.1"
                     /translation="MKKLKIIIPVFVIVSIAVIFWTIAGLAFIYSTVDRDKMFKYYQI
                     ISAKYYENFEEDSLNEVNSAVINYQDLYDVKAYSLKLSFDIPDKKIFGIMNMRSLCLS
                     DTLNFIYIDLSDDLKINSIKINGDELSKSSYKRNDNLIVADSRGLIDKDEEFSVEINY
                     EGKPINKGFDSFNIKNFDNEPSIYTLSEPNHAKTWWPCKDVLTDKADVEVIITVPKKL
                     TAVSNGKLENILTDEAGMKTFHWRSAYPTATYLVSLAIGYYDSWNDTYTSVDGNIKMP
                     VEYYTYPYLTEKAKIDWKETPEMLSFFSEKFGEYPFINEKYGMATFGWVGGAMEHQTI
                     SSMGYTLVTGNGKYEDVVVHELVHQWYGDAVTPKSWKDIWLNEGFASYGEALWVEHKN
                     GFDAYKNFMRKNDYSFFMGTLYDPEGYIFGPTIYKKGSWVLHMLRGVTGDSLFFEILK
                     TYYEKFKYQNADTYDFQKVCEDITGNDLKYFFNQWIFTGTGRPEYNYSWNFDQINKSQ
                     ENSKENSETLFKVSINLNQTQEDLEVYSMPIKILFKTNSGDEEIIVFNDKRNQQIDHT
                     LKGKPIDVIIDNDNWVLKKIKKDGNN"
     gene            10121..10828
                     /locus_tag="PLX80_04855"
     CDS             10121..10828
                     /locus_tag="PLX80_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20158.1"
                     /translation="MGKSNLLFTLITLILFSSVFSSCISISRNIKVNSDGTGSEILNL
                     TFKKEFYDVMASMTSLMDSTRRQGFLDSLYSDEVFENKTIEKYDSIQGIKVIDLNTVM
                     NSDSSKTFTIDYEFDDIKKIGSSLNYINENDDKYKTDVIFKEDNGKVTFLYLYEMKGD
                     DLSEELGDNDSLAQKMKLSMAMMFEGGDFDFEVEMPYDVISTNATEQDGRKLKWHYQM
                     SDIFTKDKISLEAEMQK"
     gene            10999..11490
                     /locus_tag="PLX80_04860"
     CDS             10999..11490
                     /locus_tag="PLX80_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20159.1"
                     /translation="MIKNYLIFTVCAMMFVVLSSCTKEVPPELLKNNNSSSNTTQKQE
                     MPDDSIHRNLKNNSESGNTGGEESWNDEGANKLIKEADAADAAYVKTKSDKDKETCVN
                     MQLKAANYLMFDADLPPKDKYKPALQRYRRVLELDPGNQEAMVNKQQIEEIYQSMGKP
                     IPN"
     gene            11528..11920
                     /locus_tag="PLX80_04865"
     CDS             11528..11920
                     /locus_tag="PLX80_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQY20160.1"
                     /translation="MNKKTIIGVLLIAVFLVVGFLTFTESNIEYTNFDQASGSHRTCQ
                     VKGYWQKDKEAKYDAATNQFTFYMLDESNKEMKVVLDGSRPNNFEMAESVVAKGKVKD
                     GNFYAKEVLTKCPSKYEGEGHEVKKTGT"
     gene            11939..14317
                     /locus_tag="PLX80_04870"
     CDS             11939..14317
                     /locus_tag="PLX80_04870"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HQY20161.1"
                     /translation="MGHFFITAGFISGLVATAAYFFVSRGNEKLLKPARIFFHLAVVM
                     TVASAAFLIYLIITHQFQYTYVWNYSSKDLPINLLISTFYAGQEGSFHLWAFLTAVIG
                     IFLHSYLIKRDSENAKAEHSHKDDFEPLVMLSYSLILTFLLFIMIIKSPYMLVWESFP
                     KDVEAGFIPQDGRGLNPLLQNFWMTIHPPILFTGFSSLAIPFAFALAALFKNRYDRWM
                     KLALPWTLFSGMILGVGIMLGGFWAYGVLGWGGYWGWDPVENSSLVPWIIIVAAIHTM
                     VGEEKTGKYKKTSLLLCILGFVFMLYSTFMTRSGLLGDASVHSFVDPGQEVYLFLIVF
                     LSLFGGGGIAMILYRLKSLKSLSTNAGIMLSRESALIIGAITLCATALIISVGTSWPI
                     FTKATVDPAFYNKMNLPLAIIIALVNGISILLAWKHTDHKRFLKSLYMPVILTVIFTT
                     GLVILGIDDILIAVFAASALFAFFINLNIIFKILTKSTVKAGPYIAHLGIMILFLGII
                     GSSKYSQEENVSLPLGESKEALGYNLTYKGASLIPGTEDKYHFNVIVEKEGKGYLLQP
                     IMYYSDYSEGIMKNPDIANLVTKDIYLEPISLETPEPYSAEDVVNLTKGSEKEIKGVN
                     VKFIEFDQSKFNQESMAKGEENILGAKLEITMDGKTEELIVEQYFSKGQTQNIPAKIE
                     SNDKFTFYLTKISIAGESGIEIAVAEDHQHSTHSSETLILTASIKPFINLVWGGTIIM
                     ALGFFASLLSRYRRFKGPKVKSPEGNQNGHSNGHNSVHSKNGHEKVHAEHEN"
     gene            14426..15223
                     /locus_tag="PLX80_04875"
     CDS             14426..15223
                     /locus_tag="PLX80_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQY20162.1"
                     /translation="MKLILYIQAFLFFISAYQNSCGNTEEHIKQKDSTEKVLSIPDGL
                     QKLVNAYPEFLEKADENNLYWKDGTVMIYDDGIEDKTHDEMLNDSDLEDMMSQKYTKG
                     RNWNEPPPENFEPGRIRYEPFFLKMYGNNSNEVSSEIVNVTWVDGSQISFSNVNGAAD
                     SLEKVAEELSQLPGEFHKYLKNIGGTFVWRNIAGTDRLSNHSFGTAIDINTMYSDYWK
                     WNKNVTYVNRIPIEIAEVFEKYGFIWGGKWYHYDTMHFEFRPELTAD"
     gene            15356..15523
                     /locus_tag="PLX80_04880"
     CDS             15356..15523
                     /locus_tag="PLX80_04880"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HQY20163.1"
                     /translation="MTIKLLNDEHSPVVKWKVRDSFPVNYSGLVLEANSSEIAMEVLE
                     ISHERLTVETV"
     gene            complement(15923..16798)
                     /locus_tag="PLX80_04885"
     CDS             complement(15923..16798)
                     /locus_tag="PLX80_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HQY20164.1"
                     /translation="MNKLKIFYLALLTVLSTAIIYNGCSEVQNDLVTSPEVYVHPPGW
                     ADGDTPDFHGNFINDNKQWNLQVCQSCHGNDYAGGSSGSSCLTCHTSSGGPQNCRLCH
                     GGVSGRSYPPKALNGETSVSYIGVGVHSYHIDSTQYSASVECNECHTPLTSGFNSPDH
                     IGNFPDGIAEVNFGSLSKTQTTFKGGEINPDPVWDRNTATCSGSYCHGNFRDGNTEAV
                     PVWTDKNSVKCGSCHGDPVTGNPNPIPNGNFFHPHYPSYTINICYQCHGSVIDQAGSI
                     INKSKHVNGVVDYNN"
     gene            complement(16847..18127)
                     /locus_tag="PLX80_04890"
     CDS             complement(16847..18127)
                     /locus_tag="PLX80_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQY20165.1"
                     /translation="MKTKFIVYYILFLTGIMIFLTTTAYLTNEETEAMKLNEVFNISA
                     VKNTNENSTKYDNSKLIKFNHELHSQGAEIKCADCHTAAVNSTSAKDNLNPTKKNCEE
                     CHDVNDEKECGLCHYDNVYKKLRSSDSELYFSHKEHNSQQQCVDCHKDMDKVKFSKES
                     SQVMPTMESCYSCHNNKKAANECEVCHTNLTNLTPKDHLQSNFLNEHTSKVAFSEKNN
                     CMMCHSDNFCQACHSPLKYSGNNTPEDFYVPYYTKETGTRTDRGALQKLTTAHTLNYK
                     FTHGLDADSKSFECKTCHNPTDFCASCHSNGGDLITGISPKSHSQPNFTTVGVNTGGG
                     LHSDLAKRDIESCQSCHDVQGSDPVCVQCHFDNDGIEGTNPRTHQSGYLNDEKGIWHD
                     TQGAVCYTCHTDPNARPDGVAGVGFCGYCHGMER"
     gene            complement(18138..19439)
                     /locus_tag="PLX80_04895"
     CDS             complement(18138..19439)
                     /locus_tag="PLX80_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20166.1"
                     /translation="MKTYYALKPLIILMLLTCTFSVNAQSLNLRLSAQVYGWQRADSI
                     GSTNKTSHLRSYENILMEVRQDKWTFNTLIQTDADLMNQIGKGFDYRFYNLYIKGANL
                     FDVLDMKIGRQYLFSGVESGAMDGLYLKLKAGANKEYQFTAFGGSITEQDYEFTGYAP
                     FSENSIYGGNFSYYGLRDFFAGVSYVNKHNKPEPYSAIRYDTLFNPYETTISLDSPTD
                     QLIGLDMNYTYLKKHFFFGKFYYDFNNRKIYRGEFNATVTASDNFNVTGFYTYNQPQI
                     RYNSIFSTFDQSEFQEAGGGVNYSFSNGINAYAKVSDVIYTNDNSVKVQAGINNPSYG
                     LAYVYYGGYSGESNGVNGYYYRQLIEKILSMNLSLNFSSYKLGEYSTEFQSAFSGSLG
                     LTYRPNLQFSIDLQGQLITNEIYKTDSRFIVGINYWLFTKF"
     gene            complement(19461..20045)
                     /locus_tag="PLX80_04900"
     CDS             complement(19461..20045)
                     /locus_tag="PLX80_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HQY20167.1"
                     /translation="MKYLILLIIPMVILINILSGSENISKEKYKYVGVNSCVSSCHKS
                     ESQGNQEKIWSESKHSKAFETLQTIRADSIALSKGFNTPAVETPECVKCHTTGSITDV
                     SEFQETFDITQGVQCESCHGPGSEYKSLSVMKDKERAFSLGLIIHNEKEKFCIQCHNT
                     ESPTYFQFDYEPMWEMIAHPTPKNIDSENESETD"
     gene            complement(20122..>20430)
                     /locus_tag="PLX80_04905"
     CDS             complement(20122..>20430)
                     /locus_tag="PLX80_04905"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQY20168.1"
                     /translation="AETPECVVCHVLGREMMPEALEVTFDKTEGVQCESCHGPGSEYK
                     KLSIMKDKQKSIDAGLIVHTDGENFCTNCHNSNSPTFVEFNYAEMWDKIKHPKPAGVE
                     "
BASE COUNT         6667 a         3603 c         3374 g         6786 t
ORIGIN      
        1 tatctcgaag caaaagatga aggacatggc tttgcaaaga aagtaaatgc ggattttcag
       61 aggtatgtta ctgtgatgtt tgtaaagaag tatttgcttg agggagttga gttttgagaa
      121 acggttttaa ccgtttctgg ggtgaagtcg gagaatgttg ttattatatg taaatgtata
      181 ttttgttaag aagactctaa acatgagaca ggcaataatg ttgtagatga tgtcagtgga
      241 taaacggttt taaccgtttc tactctctga aaaaagaaag agcaggataa atttcaccct
      301 gctctgttcg ctgattcttt tggggaatta tcagctgttt atcatgaaac atacttttgt
      361 ttcacgtcca aaaaaattct aaggtctgat ctttactaca aagttaaatg aattattatc
      421 agcaattgcc tggtcagaag catcaacaaa attatctcca tcaagatcag ttacttcata
      481 tccctgagca aatattgaaa tatcattatc taccatgcta acgtcagtta catccactgc
      541 tccgtcctga ttaacgtctc ctgaataaat acaatattca cctcctttta atttgagatt
      601 atctccgtaa gcttttgttt ttgaagttgt aaaattataa cttaaaggat aaccaagctg
      661 gaattgctgt actgtcttag cccatgtctc gatggaattc ctgtggcgga tcaatatgta
      721 ataataaata ccgcttgatg catttgggaa gtttgcaatg catgttccgt ttgaaagagt
      781 taatacttta tcataatcaa caagtgcata tggagacaat ggagatctta acaaaaccgt
      841 tatagtatct tcttccatac tagaaccgtt atatctccct tctaaatatg atttcaattc
      901 aagtgaaaca atcccgtttc ctgatgaatg attctttgtt gtatctataa gtttataaag
      961 catagtatca cgtagacttg cacttccgcc catacttcca ataactttaa aatcatatga
     1021 tctgaatggc tggtcaaatg ttgatatggt taagattgaa tcattggtca gcgaaacatt
     1081 tggaggtgca ttcaccagct ggtactgcat gtttgctctt acaggtctta ctctgagagt
     1141 atctctctga acgaatatag atctcctgaa acatttagcg gcaacaggat acattctcgg
     1201 acggtttgat gttagaagct gcacgtcaaa aaaatatctt cccttttcta cattgtcata
     1261 tagaactctt acataccagt caccggttgt acctattcct acattaaaag tcggtgtcca
     1321 ccaggaccat gcaaattgtg cctgactatt aaggtaaaaa taagtatcat caaataaaga
     1381 accatctgat cttctaattt catatcttat ctgctttcct gtattatttc cctgaagctg
     1441 cagccagaaa ccaatcctgc cttcatatcc gctgactgta tttggagtaa aaattctttc
     1501 cttaaaataa gagataggaa tattatccat atctcctcct atcagactcg aagtaaaaac
     1561 acccatatcg tgtaatttaa aagctgtatt tcctacatat gcttcctgat cagcccattg
     1621 tgaaggcaga tgattataat ctccctgcca gggatctctt ttgttccaag ttccatttac
     1681 aaaatatcct gattcgaaat gtaggtgtgc atctgtagaa actccggaac ttccaacata
     1741 tccaattatt cttcctttct gtacatactc accgagtttt acagttactg attttttcat
     1801 aagatgataa taatatgcat agctcccgtc gtcatgtttt ataagtattt tattggcagg
     1861 atctacatta ctccatccgg tatttctgtc aaaattattc attactgtct gtatgaccac
     1921 tcctgattca gcagctttaa tagcataaaa tctgtccatg ttacgaaaat catgcagaca
     1981 aatgtcagtt ccgttatgac cgttatatga ccagtcattg tcattataat cctttatact
     2041 gccacctgca agatcatcta cataatttac tataactaat ccttcattaa gtgctgcttt
     2101 taatggccag tcatatgaag gtcctcccat atttaaaggt cctgaaatgt tttcatgtat
     2161 tgcaggatca aatttaattt cttccgcggt acagtttggc tgtgatgaat ttttatcgga
     2221 gtaaagataa agttcatctc cgatcttctc agactgaccg aatgtcaata ccgagatttg
     2281 tagcattaat aaaattataa ctgttttcat gataaatgat tttaattaat aaacttcaca
     2341 aaattaatac tgtctggtat attttccaaa gaattatttc accaaaaatc accagtaaaa
     2401 aggaatctac ttactgattt agtccaaata taacgtgttt tcattgtttt ccttagaaaa
     2461 taatgttttt ggaatcatta catttatcaa aaaaatagac aggaaaaaac aaaatttata
     2521 tataattccg gtaattgaaa cgtatgatta taaaaacttt tgatacagca aattttttga
     2581 atttatgttt aatttataaa gataattatt tttataaaga atttgagatt tcaaaggttt
     2641 atatttgcaa ttgaaattta tagagagttg tccgagtggt tgaaggagca cgcctggaaa
     2701 gtgtgtaagg tcgcaaggtc ttcgcgggtt cgaatcccgc actctctgct gttaaattta
     2761 atactttact ttaatgcaat ttttaaagga taaaaaatga tttttcaata tccctcacac
     2821 aatattgttt gttcaaaaca atttaatatt ttcaaaggaa atcaaacaaa aacttaacat
     2881 caggaataaa gtattttcta atttataata atttaatatt taaaatttag aatttaatat
     2941 ttaaaattta gaatttaata ttggagaggt ggcagagcgg ttgaatgcgg cagtctcgaa
     3001 aactgttgta ggggtaactc tatcgagggt tcgaatccct ccttctccgc atttataaaa
     3061 ctacttcatt tcaatttttc ttcaaaaaat ttcacctttt ttactctaaa actttatcac
     3121 acccggaata gacaataaag cattctactg caaaaatgtc tttgataaac ttcttccaat
     3181 caacggagat tatgacagaa atatcaaaaa ccatttaaat agtgcaattg aaatagactt
     3241 tgaaggaaca atattggaat tgaaatcaga taaatgtaaa aatgtatagt tagagttcga
     3301 ataataattt taataagaaa taatgtaaat ttgtttttag taatgcgtta aaccctgaaa
     3361 aaatggtatt attaattaaa gattgatttt taaataaatc aatgttattt ttgttataat
     3421 tattttttca aaatcaaaca ataaataaca tgacaaaaga agaactggtt accaaaatgg
     3481 caggagatgc agagatcaca aaagttcagg caagagcagc tttagaatca ataattgacg
     3541 gtgtaaaaaa gacgttaaaa aaaggtggta agctttcact tgtaggattc ggaactttct
     3601 caatttcaaa aagaaaagca agattaggaa gaaaccccca gactggaaaa gagattaaaa
     3661 ttgctgcaaa aaaagttgca aaattcaaag caggaaagac tcttgcagat cacgtaaaat
     3721 aattataatt agtattaata gaaaaggcgg ctgtcatgcc gccttttttt atgctcaaca
     3781 aaaatttact ttactactgc aaacttacct gtaaattctt caacatcctg accgtcggaa
     3841 ttttttccgg atgcaacata aatgtaaata ccggttggta ctgcatttcc accactatcc
     3901 attaaatccc actccactcc gccattgccg tctgcttctg ctattgttaa aataaatttt
     3961 cctttcagat cataaatatc gatactggca tttcttgtga tattggcaaa cgtaatataa
     4021 tcctgacccg tgctgacact gtatggattt ggatatacat acatttcatt caggttttct
     4081 ttgttaaaaa taagacccag actgcttccc gctccgtcaa ctatctttat acctccagat
     4141 gaatatacat tttttacatt catgatataa ttttttcctg ttgcacctat aactgaattt
     4201 ttctctaagt caatataaac tttttttctg tctgcattat caattgttac agaactcact
     4261 gaaatattca aaggctgaaa tttataattc gatgtattcc tcccacttac agaatcgact
     4321 tccatattaa attcagcagt taatctgtat tcaccaatta actcgagttt ggataaataa
     4381 aattttatag aatctgtttc cagtactgta aagcttttcg tattcgtatc gacaggcgat
     4441 ccgtaataat ctgtaagcac taaagctttt acattatagt ttccattttc aggaatgctg
     4501 ttcattgtca ggaaatattc ataattactt tttgctgtaa tattctttgg attaaaaggt
     4561 acagcagaat ttcccgaaat cacaaatgaa ctcagatttg gaatcagcgt tggaattctt
     4621 tctgaatatt ttagtacaag aaaaccgccg ttttgatatt ccgctgaaag cagttgtgat
     4681 ttattatgaa catatacttt cacaggatcc gttaacatac tttctttaac agaattttgc
     4741 tcatctactg aagatatttt ataataataa tttttcctgt ttaaaataat cagatcgata
     4801 aataaattag taaccacaga atcatacaaa gaaaaattca ttgtcgaatc acttttgtaa
     4861 accttatatc tttcagcacc ggagacaggt tcaaatgtaa gctccactga gttggaatca
     4921 atactatgac ctttgagatt cagaggtgtc gccggtcctg taaaagcaat atctttttca
     4981 aagaaaataa gggagtcatc aacagtattt gcaccgatct cttttacacc gtcaccatcg
     5041 aaatcaaatg ctatctgatt aaatgaattc attccttctc tgtaataatc aggcataaat
     5101 ctttgaagtg tcgtgttgaa tttcaaaatg taaaataaag tcccgacatt tacaatcact
     5161 tcatctctgt tgtcattgtc tatatcagca gataaagtag atgtttttgt ataatcttta
     5221 taattataaa tatccgtaat gtccaggagt tcatactgat tatcccccgc agaagtatat
     5281 gagtatagac tgtaatattg tataaagtca gaattctgac tggtcacacc gagcgcaaaa
     5341 tcttttttac cgtcaccgtt aaaatcacct gaaacaatat tttcaccttt tataaatctg
     5401 tatatactgt caataaaaac tctttggtaa ttattgtcgg aaacattttc ataaactgaa
     5461 acgcatgtga ttgggatacc tggatttgaa gggctgtaaa atatatttga aaacattata
     5521 tcttttttcc catcattatc aaaatcttca acaagtacaa cctgggagtt tgtgatagaa
     5581 tctgtaccat agaagttaag atttgcagtc tgattaaaat tattaccgga tgttgattca
     5641 agaattctga gtccgctgtt accaaatcca agtaattcat ttttaccgtc actgtcagta
     5701 tcagcgatca ttgacgacca gaagtttccg tttccctgat cactccagac aaggtcagta
     5761 ggtatggcat tctgatttgg agcttcatag agaaatccgt ttctgtcgct cgaagcaagt
     5821 atctcaagtt taccgtctcc gtcaacatct gcaatatctc tcgcgatctt aaaatctccc
     5881 cagttgtcat tcgagatctt ctgaaaatta ccattactga attcatatac attcagctta
     5941 agattattat taatatcatt tacaagaaga tcattaagac cgtttccatt tatgtcagtt
     6001 accgttcttg caaactgtga atttccaagt ttataatttt tgttattaaa tccgtaatta
     6061 tttatagacg gctgtgtata aaaataaaat gccgtatcac taaatgttgc agacttgccg
     6121 tttagagaaa tggcttcaac ataaaattca tattccgtat tcggagcaag gttatttccg
     6181 tttagaagtc cgaaatgtgc attggttaca aagccgatat tcggagtgcc gatgtctgca
     6241 agaactgaaa tatacggctc acttgaacct ttacgtttat aataaatttt gccgagcgta
     6301 cgtttatttg tagcaaataa aatcaattga gaaaaattat tcttatcaat cagatttcca
     6361 aactgaatat cggtaaacat tggtggattc ttatccttaa aaattatcat gcggtgttca
     6421 atagtcctgc ctgaattact atttatagca agtcgcaaag tatatgacgt atcctgaagt
     6481 ccggctgtat tccactttaa gactgtatct ccgagaacct gagaagtctg atttgaaagt
     6541 agcggaatcc attccgaagg tctctgtcct tttgcataat atagtgagta tgataaaaat
     6601 aaatttgatg cagcagagac actgactgta acagtatcat tttcaaaggt ataatcctga
     6661 gacggcgtga ggattcttga aatggtcgga ttattaaaat tctgaagagc attcaacgca
     6721 tcgacccttc ccgaagatct gacatgatcc catgaatttt gtccttgcag catagccgta
     6781 gtagaaacaa gaatacccct tatctcttca ttagtaagag aaggattttt tgaaagaagc
     6841 attccggaaa tccctgcaac gatcggcgct gcaaatgaag tgccgtttaa tttattataa
     6901 tcaccgccgt attgcgaact gcctttgccg acacggactg tagttaaatt ctgaaaaccc
     6961 ggtgcaaaaa tatcaactgt ctcaccgtag gaagaaaaac ttgctttatt atttgaattg
     7021 tctgaagctc cgacagaaac cacttcatca taagctgaag ggtaatgaag tctgtctgta
     7081 ccgtcatttc ctgctgagca aataatcaga acattctgta aatatgcaaa tctaattaca
     7141 tcttttaaaa gattactgaa tacataatct ccaaaactga aattgaaaac ttttacacca
     7201 ttggcaactc cgtataaaat tgcatttgca acatcgtctt cttctccgta accttcagca
     7261 tcaaaagctc taagcaccat tactttacag ccgggtgcta cagatgaaat acctgtaatg
     7321 ttgttgaatc ctgcattgat aattcctgtt actgcagtac catgtgagac actgttatca
     7381 tcagtcggat cattatcagg gtcaagataa tcacctcttc tcggatcgcc tgtcaacggt
     7441 tcgtctgtaa aatcccatcc tctccagtca tcaatgaatc cgttattatc atcatctatt
     7501 ccgtttgact ctttgccatt gccgtattca ccgtaattaa ttttaaaact gttctgaaga
     7561 tccggatgca gaaaatccag accggtatca attacgccaa tgactatatt agaatctccc
     7621 aaagtaatat cccatgcatt attcattcca atcatattga ggtaattttg atcagagtaa
     7681 tatggatcat ttggtgtaac aggaggacct tggatatcat caactttcag aacattattg
     7741 aactgaatat attctataaa ttcattttta ccagcagcag tcagaagcag atcaagattt
     7801 tcattattag tttcagaaat aaaaattctg ttgagacctg tttttgatat agcatccgac
     7861 aaattcctgt tatcaaaatt ttgcaaaacc ctttcattga aaacaggttt tgactttttc
     7921 aaaccaaagt ccctgctaag tttcgacaca gtagaattgc ctgattcggg tctgttattt
     7981 ttaaagtttg aaattactga agcaggtgtg ttttctttga attttataat tacagttgtt
     8041 tgtgaaaata ctgtatggct gcagaaaaga atagctaata caaaaagtgt tgttaatatt
     8101 tttacggaat tcatataatt gatagatgac ctttttttga atttaagaat gctgtaatta
     8161 attgttaccg tcctttttaa tttttttcaa aacccagtta tcattatcta taataacatc
     8221 tataggttta ccttttaaag tatgatcgat ctgctgattt cttttatcat taaaaactat
     8281 gatctcttca tctcctgagt ttgttttaaa caatatcttt attggcatgg aatatacttc
     8341 caaatcttcc tgtgtttgat ttaaatttat tgatactttg aataaagttt cagaattttc
     8401 ttttgagttt tcctgtgatt tatttatctg atcgaaattc catgagtaat tatattcggg
     8461 tcgtcctgtg ccggtaaata tccattgatt gaaaaaatat ttcaagtcat ttcccgtaat
     8521 gtcctcacat actttttgaa aatcataagt atcagcattc tgatatttga atttttcata
     8581 ataagttttg agaatttcaa agaagagtga atctcccgta acacctctaa gcatatgtag
     8641 aacccagctt ccttttttat atattgtagg accgaagatg taaccctcag gatcataaag
     8701 agtgcccata aaaaacgaat aatcattttt tctcataaaa tttttatatg catcaaatcc
     8761 atttttatgt tcgacccaaa gagcttctcc ataagaggcg aaaccttcat tcagccagat
     8821 gtccttccat gatttcggcg tcacagcatc tccataccat tgatggacaa gttcatgaac
     8881 tacaacatct tcatatttcc cgtttccggt aacgagtgta tatcccatac ttgaaatagt
     8941 ctgatgctcc atcgcacctc cgacccagcc gaatgtagcc atcccgtatt tttcatttat
     9001 gaatggatac tcaccgaatt tttcagaaaa gaaacttaac atttcaggag tttctttcca
     9061 gtcgatcttt gctttttctg ttaagtatgg atatgtataa tattcgacag gcatttttat
     9121 gtttccgtca acagaagtat aagtatcatt ccaactgtca taatatccga tcgcaagtga
     9181 aaccagataa gtagctgtag gataagccga tctccagtga aaagttttca ttcctgcttc
     9241 atctgtcaaa atattctcaa gctttccgtt tgaaactgct gtgagttttt tcgggacggt
     9301 aataataact tcgacatcag ctttatcagt taatacatct ttacagggcc accaggtctt
     9361 tgcgtgattt ggttcgctta aagtataaat agaaggttca ttgtcaaaat ttttaatatt
     9421 gaaagaatcg aagcctttgt ttattggttt accttcataa ttaatttcga cactgaattc
     9481 ttcatcttta tcaatcaatc ccctgctgtc agcaacaatt aaattatcat tccttttgta
     9541 tgagcttttt gaaagttcat caccatttat tttaattgaa tttattttaa gatcatcact
     9601 aaggtctata taaataaaat tcagagtatc tgacaaacag agagatctca tattcataat
     9661 tccgaatatt tttttatcgg ggatatcaaa tgataattta agtgaatagg ctttgacatc
     9721 atatagatcc tgataattaa taacagcgga attaacttca ttcaatgaat cttcttcaaa
     9781 attctcataa tattttgccg aaatgatctg ataatattta aacatcttat ctctgtctac
     9841 agtcgaataa ataaatgcaa gccctgcaat cgtccagaat attacagcaa tagacacaat
     9901 cacaaataca ggaataataa ttttcaattt tttcatatta tcgggagaaa gaaaggaaac
     9961 taaacactat ttagattaaa aagtttattt ggtaaaatta aagtcagtga atttcaaatt
    10021 cattctaata tactgaccaa agtcatatct ttacaaaaaa gtatctctta ttttgcaatt
    10081 aaattagtta aatttactac taatttaaaa ttaataattg atgggaaaaa gtaacttact
    10141 gtttacattg atcactttga ttttgttttc atctgtgttt tcatcttgta tctcaatcag
    10201 caggaacatt aaagtaaatt ctgacggcac cggatctgag atcctgaatc ttacttttaa
    10261 aaaagaattt tacgacgtaa tggcttcaat gacatcgctg atggattcaa cccgaagaca
    10321 gggttttcta gattcgttat acagtgatga agtttttgaa aataagacaa ttgaaaagta
    10381 tgattcaata cagggcataa aagtcattga tctaaataca gtaatgaatt ctgactcatc
    10441 aaaaacattt acaattgatt atgaattcga tgatattaaa aagatcggat cgtctttaaa
    10501 ttatattaat gagaatgatg ataaatataa gacagatgta atatttaaag aagataacgg
    10561 aaaagtaaca tttctttatt tatatgaaat gaaaggtgat gatctctcgg aagaacttgg
    10621 tgataatgat tcattagcac aaaagatgaa attaagtatg gctatgatgt ttgaaggggg
    10681 agattttgat tttgaagtcg aaatgcctta tgatgtaatc tctacaaatg caactgagca
    10741 ggatggcaga aagttgaaat ggcattatca aatgtcggat atttttacaa aagataagat
    10801 aagtctggaa gcagagatgc agaaatagta aatccccatg tggataaaga aaagtgatta
    10861 atgtggatat aatggacagt ttcaaatgga gtgaaatttt ataatgaaaa ttaagttgtt
    10921 tacagtgttt ttcttacaat aaatgtaatt tccatggtac aaaaattgag tttgaaataa
    10981 atacaaatta atttaataat gatcaaaaac tatttaatat ttacagtttg cgcaatgatg
    11041 tttgtagtac tttcatcttg tacaaaagaa gtaccgccag aacttttaaa gaacaataat
    11101 agcagttcta atacaacaca aaagcaggaa atgccggatg attcaattca cagaaattta
    11161 aagaacaata gtgaatctgg aaatacagga ggcgaggaaa gctggaatga tgaaggtgcg
    11221 aataaactga tcaaagaagc agatgcagca gatgctgctt atgtgaaaac aaaatcagat
    11281 aaagataaag agacatgtgt aaatatgcag ttgaaagctg ctaattattt aatgtttgac
    11341 gcagatcttc ctccaaaaga taaatataag ccggctttac aaagatacag aagagtactt
    11401 gaactggatc caggtaatca ggaagcaatg gtaaacaaac agcagattga ggaaatttat
    11461 cagtcgatgg gtaaaccaat accgaattaa aaattaattt ttttaaaaag attttaaata
    11521 aattatcatg aataaaaaaa caataatcgg agttttatta atagcagtat ttttagtagt
    11581 cggctttctg acttttacag aaagtaatat agaatacacg aatttcgatc aggcatccgg
    11641 aagtcacagg acctgccagg taaaaggtta ctggcaaaag gataaggaag caaaatatga
    11701 tgcagcaact aatcagttca cattttatat gttagatgaa agcaataaag aaatgaaagt
    11761 ggtactggat ggatcaagac caaataattt tgaaatggct gaatctgtag tagcaaaagg
    11821 taaagttaag gatggcaatt tttatgcaaa agaagtatta actaaatgtc cttccaagta
    11881 tgaaggcgaa ggtcatgaag taaaaaaaac aggtacataa atttaattta agttcatcat
    11941 gggtcatttt tttataacag caggatttat ttcaggatta gttgcaactg cagcatattt
    12001 ttttgtcagc agaggcaatg aaaagctctt aaagcctgca agaatatttt tccacctggc
    12061 agtagtaatg acagtagcat ctgccgcttt tttaatttat ctcattatta cacaccagtt
    12121 tcaatacact tatgtatgga attatagttc gaaagatctt ccaataaatc ttctgatctc
    12181 tacattttat gcaggtcagg agggaagttt tcatctgtgg gcatttttaa ctgcagtaat
    12241 tgggattttc cttcacagtt atctgataaa aagagattca gaaaatgcaa aagctgaaca
    12301 ctcacataag gatgattttg aaccacttgt aatgttaagt tattctctaa tacttacatt
    12361 tttattattt ataatgataa taaaatctcc ttacatgctg gtatgggaat catttccaaa
    12421 agatgtggaa gcagggttta tacctcagga cggtcgagga ttgaatcctt tactgcagaa
    12481 tttctggatg acaattcatc cgcctatttt attcacagga ttttcctcac tagcaattcc
    12541 atttgcattt gcacttgctg cattatttaa gaacagatat gacagatgga tgaagctcgc
    12601 acttccatgg actttatttt caggaatgat acttggagtt ggaattatgc ttggcggatt
    12661 ctgggcatac ggagttcttg gatggggagg ttactggggc tgggatccgg ttgagaattc
    12721 ttcattagtt ccatggataa ttatagttgc tgcaattcac acaatggtag gcgaagagaa
    12781 aacaggaaag tacaaaaaaa caagtttact actttgtata ctcggatttg tatttatgct
    12841 ttattcgaca ttcatgacaa gaagcggact tcttggagat gcatcagttc attcatttgt
    12901 agatccggga caggaagtat atttgttttt aattgttttc ctttctcttt tcggcggagg
    12961 cggaatcgca atgatccttt acagattaaa aagtttaaaa tctctttcga caaatgccgg
    13021 tattatgcta tcccgtgaat cagcattaat catcggggca atcactttat gtgcaacagc
    13081 attaataatt tcagtaggaa caagctggcc tatattcact aaagctacag tggatcctgc
    13141 tttttataat aaaatgaatt tgcctcttgc aataataatt gctttagtaa atggcataag
    13201 tatccttctg gcatggaaac atactgatca caaaagattt ttgaaatcgc tttatatgcc
    13261 ggtaatttta actgtaattt tcacaacagg acttgtaata cttggaattg atgatatttt
    13321 aatagcagtc tttgctgctt cagcattgtt tgcattcttt attaatctga atataatatt
    13381 taagatactt accaagagta ccgtaaaggc aggtccatat attgcacatc ttgggataat
    13441 gattttgttt ttgggaatta tcggttcatc aaaatattcg caggaagaaa atgtttcact
    13501 tccactaggt gaatcaaaag aagcgttagg ctataatctg acttacaaag gtgcaagtct
    13561 tattccgggt acagaagata aatatcattt taatgtaata gtggaaaaag aaggtaaagg
    13621 atatttatta caaccaatta tgtattacag tgattattca gaaggcatta tgaagaatcc
    13681 ggacattgca aaccttgtca ccaaagatat atatttagaa ccaatttcgc ttgaaactcc
    13741 ggagccgtat tcagcagagg atgtagttaa tttaaccaaa ggcagtgaaa aagaaattaa
    13801 gggagtaaat gtaaaattca tagagttcga tcagtcaaaa ttcaaccagg agtcaatggc
    13861 aaaaggcgaa gaaaatatac ttggtgctaa acttgaaatt acaatggacg gaaagactga
    13921 agaattgata gtagaacaat atttttcaaa aggtcagaca cagaatattc ctgctaagat
    13981 agaatccaat gataaattca cattttacct tacaaaaatt tcaatagcgg gtgaatcggg
    14041 gattgaaata gcagttgcag aagatcatca gcattcaact catagttcag aaacattgat
    14101 attaacagca agtataaagc cgtttataaa ccttgtctgg ggcggaacga taattatggc
    14161 attaggtttc ttcgcatcat tattgagccg ttacagaaga tttaaaggtc cgaaagtaaa
    14221 atcaccggaa ggaaatcaaa atggtcacag taacggacac aactcagttc acagtaaaaa
    14281 cggacacgaa aaagttcatg ccgaacatga gaactaaata ataaaaattt ttattcaaac
    14341 cggaatttca attctctgtt aattgtagtt ccggtttttt attttacaaa aacgatcaaa
    14401 taaaattctg aacccaattc taataatgaa attaatttta tacatacagg cttttttatt
    14461 ttttatctca gcttatcaaa acagctgcgg taatacagaa gagcatatca aacaaaaaga
    14521 cagcactgaa aaagtacttt caataccaga tggtcttcaa aaattagtaa atgcttaccc
    14581 cgaatttctt gaaaaagccg atgagaataa tctttactgg aaagatggaa ctgtaatgat
    14641 ctatgacgat ggtattgaag acaagactca tgatgaaatg ttgaatgatt ctgatcttga
    14701 ggatatgatg tcacaaaaat atacaaaagg acgcaactgg aatgaacctc ctccggaaaa
    14761 tttcgaaccg ggaagaataa gatatgagcc gttttttttg aaaatgtatg ggaataattc
    14821 caatgaagta agcagtgaga tagtaaatgt aacctgggtg gatggttcac agataagttt
    14881 cagcaatgtg aacggagcag cagacagtct ggaaaaagtt gctgaagaat tgtctcagct
    14941 tccgggtgaa tttcataaat acttaaaaaa tatcggtggt acttttgtat ggagaaatat
    15001 agctggtaca gacaggctga gtaatcattc attcgggact gcaatagata tcaacacaat
    15061 gtattcagat tactggaaat ggaataaaaa tgtaacttat gtgaacagga taccgattga
    15121 aatagcggaa gtatttgaaa aatacggatt catctgggga ggtaaatggt atcattatga
    15181 cacaatgcat tttgaattca gacctgaact tacagctgat taaaaaaaat tcaacacaag
    15241 caaaataaaa attcagagtt agtgtaatta ttttgacgga cttcacgagg tttccggact
    15301 taaaataact attgatttaa tcttcacagg aaggttcaaa tttgagggaa gagatctgac
    15361 tataaaatta ttgaatgatg aacattcacc ggtagtaaaa tggaaggtac gggattcatt
    15421 tccggtgaat tattcgggac ttgtactgga agcaaattca agtgagattg ctatggaggt
    15481 attggaaatt tcacatgaga ggctgacggt agaaacggtt taaaccgttt ctgtcagaaa
    15541 tgtcgaactt gtttttttta gttaaattta gttttggtaa atcaagttca ctacagcttt
    15601 caattcgaat tgaagggaaa atggattcgg aattaggcac tgtgttttag tttcggtata
    15661 aaatttattt ttccgttcac ttcgattaca ttcggcagaa acggtttaaa ccgtttcaga
    15721 tggatatacc acatcagtta tatcgttctg agcaaaattc atctccacct tctttagcaa
    15781 agttcacaat taaaattcta tttcaaattt caccggagga gttaattttt taaattcata
    15841 tctgacttag aagtctgttt taaccaacaa tttaaaccgc ccgacaataa acggttaaaa
    15901 ccgtttcacc aaaacctacc cactaattat tataatccac cactccattc acatgcttac
    15961 tcttatttat tatactaccc gcctgatcta tcaccgaacc atgacactga tagcaaatat
    16021 ttattgtata actcggataa tgcgggtgaa agaaatttcc gttcggtatt ggatttggat
    16081 ttccagttac tggatctcca tgacatgacc cgcattttac actgttttta tctgtccata
    16141 ccggaactgc ttctgtattt ccgtccctga aatttccatg gcaatacgat cccgaacagg
    16201 tagccgtatt cctgtcccat acaggatcag gatttatctc tcctccctta aatgtagtct
    16261 gtgtctttga caatgagccg aaattcactt ctgctattcc gtcagggaaa ttccctatat
    16321 gatcgggtga attaaatccg cttgtaagtg gagtatgaca ttcattgcat tctacacttg
    16381 ctgaatactg tgtagaatct atatgatatg aatgaactcc cactccaata tatgaaacac
    16441 tcgtctctcc gttcaatgcc ttcggtggat atgatcttcc cgaaactcca ccgtggcaca
    16501 gacgacagtt ctgtggtccg ccggatgatg tgtgacaggt cagacagctt gaacccgaac
    16561 ttccaccggc ataatcattt ccatgacaac tctgacatac ctgcaaattc cactgcttat
    16621 tatcatttat aaaattgccg tgaaaatccg gtgtatctcc gtctgcccag ccgggtggat
    16681 gaacatatac ctcaggagaa gttacaagat cattctgaac ttcactacag ccgttgtaaa
    16741 taattgccgt agataatact gttaataaag ccagataaaa tattttaagc ttattcatat
    16801 ttttaaaatt aaaattgtac cggtatattt tcaattttta atgtgttcat ctttccatcc
    16861 cgtgacaata tccgcaaaat cctactccag ctactccatc cggtcttgca ttcggatctg
    16921 tgtgacatgt ataacaaaca gctccctgtg tatcatgcca aattcctttc tcatcattaa
    16981 gatatccact ttgatgcgtc cttggatttg tcccttctat tccgtcatta tcaaagtgac
    17041 attggacaca aaccggatca cttccctgaa cgtcatgaca agactgacac gattctatat
    17101 ccctttttgc cagatctgaa tgaagtcctc ccccagtatt tacacctact gttgtaaagt
    17161 ttggttgaga atgactctta ggagaaattc ctgtaatcag atcacctcca tttgaatgac
    17221 aacttgcaca aaaatcagtc ggattatgac aggtcttgca ttcaaaagat ttgctgtctg
    17281 catccagtcc gtgtgtaaat ttatagttta atgtgtgagc agtagtcagc ttctgcaaag
    17341 ctccgcggtc tgttctagta cctgtttctt ttgtataata cggcacataa aaatcttcag
    17401 gtgtattgtt gccagaatac tttagcggac tgtgacatgc ctgacagaaa ttatcactgt
    17461 gacacatcat acaattattc ttttcagaaa atgccacctt tgatgtatgt tcattcaaaa
    17521 aatttgactg aagatgatcc ttaggcgtga gattggtaag gttagtgtga catacttcac
    17581 attcgttcgc tgccttcttg ttattgtgac aactgtaaca gctttccatt gtcggcatta
    17641 cctgagaact ttctttcgaa aatttcacct tatccatatc tttatggcaa tccacacatt
    17701 gctgttgtga attatgctcc ttatgtgaaa aataaagttc actgtcggat gatctgagct
    17761 ttttataaac attatcataa tggcaaagac cacattcttt ctcatcattt acatcatggc
    17821 attcttcaca attttttttc gtaggattta aattatcttt ggcagaagta gaatttactg
    17881 ccgctgtatg acaatcagca cattttattt cggcaccctg agaatgaagt tcatgattaa
    17941 attttatcag cttactattg tcatatttag ttgaattttc attagtattt tttactgctg
    18001 aaatattgaa tacttcattc aatttcattg cttcagtctc ttcatttgtc agataagctg
    18061 tcgttgtcag gaatatcatt atcccagtaa gaaataaaat ataataaact ataaatttcg
    18121 ttttcatttt cttttgttta aaattttgtg aacaaccagt aattaattcc gactataaat
    18181 ctcgagtctg ttttgtaaat ttcattggtg atcaattgcc cctgcaaatc tatactgaac
    18241 tgaaggtttg gtctgtatgt caatccaagt gatccgctga atgcactttg aaattccgtt
    18301 gaatattctc caagtttata actcgaaaaa tttaaactca aattcatcga caatattttt
    18361 tctattaatt gcctgtaata atatccgttg actccattag attcaccact atatcctcca
    18421 taataaacat acgcaagtcc gtacgacgga ttatttattc ctgcctgcac ctttacagaa
    18481 ttatcattag tatagatcac atcagatact tttgcatatg cattgatacc gttactgaaa
    18541 gaataattca ctcccccgcc ggcttcctga aattcagact gatcaaatgt actgaatata
    18601 ctattgtatc ttatctgtgg ctgattgtat gtataaaatc ctgtgacatt aaaattatct
    18661 gaagccgtta cagttgcgtt aaattcacct ctgtaaattt tcctgttatt aaaatcataa
    18721 taaaattttc caaagaaaaa atgtttcttt aaatatgtat aattcatgtc cagacctatt
    18781 agctgatctg taggtgaatc aagacttatt gttgtttcat atggattaaa taaagtgtca
    18841 tatcttatgg ctgagtatgg ctcaggttta ttatgcttat tcacataact aacccctgcg
    18901 aaaaaatctc ttaaaccata atatgaaaaa tttcccccgt aaatactgtt ctcgctaaaa
    18961 ggcgcatatc ccgtaaattc ataatcctgt tcagtgatcg atcctccgaa tgccgtaaac
    19021 tgatattcct tatttgctcc tgctttcagc ttcagataaa gtccgtccat tgctccagac
    19081 tctacacccg aaaataaata ctgtctcccg attttcatat ctaatacatc aaataaattt
    19141 gctcccttta tgtaaagatt ataaaatctg taatcaaatc cttttccgat ctggttcatt
    19201 aaatcagcat ctgtctgtat gagtgtatta aaagtccatt tatcctgtct gacctccatt
    19261 agaatattct cataacttct gagatgactg gttttattag tactgccgat gctgtctgct
    19321 ctctgccatc cgtaaacctg tgcacttaat cttaaattca gagactgagc atttacagaa
    19381 aaagtacatg tcaacagcat taaaattata agcggtttca aagcgtaata agttttcata
    19441 atattttcct gttcattttc ttaatctgtt tctgattcat tttcagaatc aatatttttt
    19501 ggtgtcgggt gagcaatcat ttcccacatc ggctcataat caaattgaaa atatgttggg
    19561 ctttccgtgt tgtgacattg aatacaaaat ttttcctttt cattatgaat tataagacct
    19621 aatgaaaatg ctctctcttt atctttcatc actgaaagag atttgtattc tgatccgggt
    19681 ccgtgacaac tttcacactg cacaccctgc gttatatcaa atgtttcctg aaactcactt
    19741 acatcagtaa tacttcctgt tgtatggcat tttacacatt caggtgtctc aaccgcagga
    19801 gtgttaaagc cttttgataa tgcaatactg tcagcacgaa ttgtctgaag cgtttcaaaa
    19861 gcttttgaat gttttgattc actccagatc ttttcctgat ttccctgtga ttcagatttg
    19921 tgacatgagc tgacacatga atttactcct acatacttat acttttcctt tgatatgttt
    19981 tctgatcctg ataaaatatt tatcagaatg accatcggaa taataagcaa aattaaatac
    20041 ttcatattgt gcatacctat tttattaatg aaaagctatt tccatttttt ataaaaaacc
    20101 caatacaagt cagagtaaaa tttactctac tcctgccggt tttggatgct ttattttatc
    20161 ccacatttca gcataattaa actcaacgaa agttggagag tttgaattat gacaatttgt
    20221 acaaaaattt tcaccatcgg tatgaacaat aagcccagca tcaattgatt tctgcttatc
    20281 tttcattatc gaaagttttt tgtattcaga accgggtcca tggcatgatt cgcactgtac
    20341 accttctgtt ttatcaaacg tgacttctaa tgcttccggc atcatttctc ttccaagtac
    20401 atgacatact acgcattcag gtgtttctgc
//
LOCUS       DAORVQ010000037        20405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1029_length_20405_cov_6.593366, whole genome shotgun sequence.
ACCESSION   DAORVQ010000037 DAORVQ010000000
VERSION     DAORVQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 20405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20405
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_20405_cov_6.593366"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..2158
                     /locus_tag="PLX80_04910"
     CDS             38..2158
                     /locus_tag="PLX80_04910"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20169.1"
                     /translation="MICRLLTFIFILLITFQSVKLSYSQSSWFWQQPLPAGNQLKGIY
                     FVTPEIGYAVGVLGTIVKTSNGGTNWQTLVSNVTVELAGVYFINENTGMAVGDSGTII
                     RTSNGGDNWNIIPSTAQYLLQDVMFPSDLVGYITGGEGIFKTTNGGINWVMQTNYGGT
                     SVNFTNDLFGAVGGTQTLVYTTNGGVNWINSEINFSFLDQVIGVSQTDTNTIIAVTNS
                     SDRVLKTTNTGMNWIEYSFGPTLNDIPRETSFVNSNTGYMVTDFGKIYKTVNGGINWT
                     IDSGFETSYRRQGVFWDICTHNSSIVGITGGGGTIIRSTNSGGNWTVQTGNLRTIKGS
                     FFINESTGYNVGYEGTILKTTNKGTNWITQSSGVTSHLNSVFFTDLNTGYAAGNEGRI
                     LKTTNAGSNWTMQFSGMMNNINSITFVNDSFGICAADSGKIVKTLNGGSNWLPEYILP
                     QQHQLFSVAFMDMNTGIVTGENGSSIRTTNGGLSWSYNVISFNTLYNVCYADSSIFFT
                     VGTNGAIHKSSNAGINWTLQSGNSTASLNSIKFLNTTNGFAAGRDGTVIKTTNGGVSW
                     VNQTKITNYELYAVSILNNNEAIIAGNYGVTLKTTNGGLVFTNNQYTLVKDDYRINSV
                     YPNPFNPSAKINYSISKTSDVSIKIFDINGREITELVNSIQSNGSYEISLDGSKNNLS
                     TGIYFVSLIVNGNLMDSKKIILMK"
     gene            2396..3718
                     /locus_tag="PLX80_04915"
     CDS             2396..3718
                     /locus_tag="PLX80_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20170.1"
                     /translation="MKKSIFTFVFIFLFILTTGISYSIQGWYELPQISANTMNSIYFI
                     DENTGFACGNNGSLFKTTDGALTWQPAGSPGNFEYSNIFFTDNQKGILTTVPMGSEAG
                     YWFRMFVTSDQGVSWTETQQYYNFISDLYNDNSGFYFSIQGFSNFETLGGLFHTQNNS
                     MNFVSIPFFNYFRNSSVFSVCRSNNRTWISAYYGDDVGTDVNRLGYTADNGSSWNIVL
                     NDSGVAWKQDPMHNKFYKIRFINESIGFLSSKLGLMKTLNGGDNWSFIDTTITKGIQT
                     HFFTSADTGWVYRLNNIYKTTNGGENFELQFIYNSYLTQPFFINSLTGYILCSNQKIL
                     KTITGGLTDVQNANSQNIPNTFSLSQNYPNPFNPKSVINYELQFTGLAKLKVYDVLGN
                     EVAVLVNEKKNAGSYSVEFDGNGFASGVYFYKLEAGDFAETKRMILLK"
     gene            complement(4074..5717)
                     /locus_tag="PLX80_04920"
     CDS             complement(4074..5717)
                     /locus_tag="PLX80_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY20171.1"
                     /translation="MKAIIILFFILIYDSAVSQLNEEWVRSFNGPVNGNDEALTHVTD
                     NSGNVYSSGKILGSGSGFDIYTVKYNPSGNILWERIYNGPGNGNDIAYSIELDNTGNV
                     FVAGESMGSGTDKDFVLIKYNDLGAEQWNRRYNGNSSSAETPEKAVTDEAGNVIITGT
                     SHEPGVLFDVVTIKYNSDGDLLWKKHFNGASSGNDFADDMAVDKSGNIFVCGSTFEAA
                     TINDYLIIKYSAGGDELWHKTYNGTANGNDNMTSITADVSGNAVITGTSVGAGTGLDI
                     ATIKYSPAGDVIWERRFTTSTVNIEEGKAITSDNLGNIFVTGTSTGFSTSYDYMTIMY
                     SPGGDLIWSKNYNGPGSNNFDEPRSIAADNNGNVYVAGLSQGTGTMDDMVIINYNYSG
                     NEVWVNRFNSAADRNDVANNISLDNSGNIIVSGFISGVTSGMDFSVVKFSVITNVLSV
                     SNETPSAYSLSQNYPNPFNPSTKIRFDIMNDDKTGAHDVRLSVYDILGKQAAVLVNKK
                     LSAGSYEAEFDASDLSGGIYFYRLETVGFTETKSMLLLK"
     gene            complement(6067..8811)
                     /locus_tag="PLX80_04925"
     CDS             complement(6067..8811)
                     /locus_tag="PLX80_04925"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20172.1"
                     /translation="MKKLYIRIFTVAIILFTNSESDLSAQWDTISTGTNADLYAVQFI
                     NVNTGWIAGTSGKIQKTTNGGTSWISQNINSSKTLRKIIMFDANTGYICGDSGKILKT
                     TNGGSKWNNTNTSSYTVNIHAMDFINASTGICANESRRQFKTTNSGLNWTNSNMNGVS
                     PIVSIFGIDMADANTVFASTSSNFLTNANVYKSSNFGSSYNVSLSNSSFSSATFNDVK
                     FINSSTGYAGASNNNLARTTNGGTSWTVKSFPKGINQISFPDPETGYICGEVFFKAKT
                     DDGGDNWAIDYNYKNGEGFFGISFINSATGWMCGSGGLLIKTTNGGGIPASCNIENSS
                     WVNLYENQSASNQSVTDDCGNIFITGYTGSSNRDILTQKYSSSGYLYWSNTYNGPGNG
                     HDEGLKIIINDYGNLYVAGNATGSATGKDLVLIKYLSDGQREWVRTYDTLNHTDEIVD
                     LEIDNSGNPIIIGNSTHPTEYDNFIKILKYNSNGNLQWVRKIGNDNYVYARAMVCNST
                     DDIYLAYDNSVSFNPALGVITKLNSSGTLQWSVNDAYYVIKELVIGNSGNLYVTGNDN
                     KLLTKKYNTSGILQWETYLNGSFTQEYYPYSMTISNGGQILITGVFNNKTFTVKYDSD
                     LALQWSHIYDNPHYTHYSPSDITTDIYENIYIIGFCSNNYLSNTYDMLTMKYNSTGTT
                     VNMKIYGDSAYGERGNSINIDNAGNVFAAGYRSIPTSQSAIIKYSPSDFSDISTLNLT
                     ALIQGFYNSSSDLMIQDTVRVYLRNNASPYSKVDSAVSILSNTGGSAFIFSNAVNSVN
                     YYLVFKHRNSIETWSKTAQQFTSNSMTYNFSTANTQAYGDNMIQADNAPVVYAVYSGD
                     VNQDGTIDLTDGSLIDNDAFNFNSGYLPTDVNGDGIIDVSDAVYADNNGFNFVGKITP
                     "
     gene            complement(8955..13454)
                     /locus_tag="PLX80_04930"
     CDS             complement(8955..13454)
                     /locus_tag="PLX80_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20173.1"
                     /translation="MKKLILFILSLTLLMIYMSTKNSPDSKSFPKENRSYERVDLKFD
                     LTQDSPFDLSVSSVQHDRSNEVKSNETSDWYSTAMSNIQKNEYDVTYNNESNTYQSPN
                     RANNMRFIYNKRGFIAKLRSNKIPLFDESDKMIEEIDKKYKYLDDWKVDFDLQSVSKD
                     LKSDLRNDYLDFNEIELSAGGNKIFIENENLRIQYSNDENGMRQDFIVKNKPVGNDRL
                     RLIINADTKLKMLTGGDALKFKDEKGDIKLQYASLKVWDANGKELRAYFEKNYELGIT
                     NYELKDKSDSKVIHNSFLIVVNDEDAAYPVTIDPLSSTAAWTAEINQANANFGWSVAT
                     AGDVNDDGYSDVIVGAPYYDNGQTDEGSVFVYHGSINGLSLSANWTMESNQSSALFGW
                     SVATAGDVNGDGYSDVIIGSPTYDSVQTDEGRAYVYYGSVSGLQAVHAWKYETNQAGE
                     NFGYSLACAGDVNNDGYSDVVIGSPFYDNGQTNEGRVYVFKGSTGGLPVTADWTYEMN
                     TLDTYLGFSVATAGDINGDNFSDITIGAPRYDIGLPDYGRVYIFHSNGNTFPATPTTT
                     RTGLQTGENFGYSVSTAGDVNGDGYSDIMIGAPLYTNGQVDEGRCYCYLGSSSGIGAA
                     AVWTQESNEISGQFGISVSTAGDLNADGFADVILGGNYLDNGQTDEGRAFVYLGYSGG
                     LSSGSAIIYESNQSNSRFGISVATAGDINGDGFSDVIVGSHMFDSPSANEGRAFVFLG
                     SAIGMSSAFVWSSESNQSNSDFGFSVSSAGDVNGDGYSDVIVGARRFDNGQTDEGAAF
                     VYHGSASGLSSFSNWSLEGNQVNAYFGTSVSSAGDVNGDGYSDVIVGAPYFDNGFTDE
                     GAAFVYHGSASGLSATAGWSGESNQESALFGLSVSGAGDVNGDGYSDLIVGARDFDNG
                     QTDEGAAFVYHGSGSGISLSFSWSSEINQANSQYGFSVSSAGDVNGDGYSDVIVGANN
                     YYNGISGEGAAFVHYGSVSGLSLSSDWSVESNQANSNFGFSVSSAGDVNGDGYSDVII
                     GANLYDNGHTNEGRAFVYNGSASGLSTIISWNAESNQANSNFGNSVSSAGDVNGDGYS
                     DVIVGAPFYDYNLENEGRVYVYHGSAAGLSSATNWTAESIIAFANLGYSVSSAGDVNG
                     DGYSDVITGAATFSNGQINEGSAFVYYGGNGISEALRSTVNQYKYGTSDIVSSDGITG
                     SNGKVRISHFAKSPFGKATVRLVSDIQQNGIPFTGGSKITNSVSYNSVSSFKNLGLTG
                     SDFTYDITGLNSEKQYKWRTRLQYTMTAYPFQKFGPWRYYNNFSSESQGGFRPKSNDV
                     NLNLTMLIQGFYDSSSDFMVQDTARVYLRNSSPPYAITDSAISILSNTGAGTFLLSNA
                     VNGTGYYIQLQHRNSIETWSKTAQQFTSSELSYNFSTFNTQAYGDNLIQADNAPVVYA
                     VYSGDVNQDGVIDIADGSLIDNDALNFNSGYLPTDVNGDDIVDVADAVYADNNGFNFV
                     VAITP"
     gene            complement(13536..13715)
                     /locus_tag="PLX80_04935"
     CDS             complement(13536..13715)
                     /locus_tag="PLX80_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20174.1"
                     /translation="MKTRIQLLTSVFLIVLVLNSREASAKLFWDQACSFNGTGTSYIA
                     VKHSSRLNLTGSFNS"
     gene            complement(14174..15655)
                     /locus_tag="PLX80_04940"
     CDS             complement(14174..15655)
                     /locus_tag="PLX80_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20175.1"
                     /translation="MIKSPAIEIIKTFSRDEFKRFVEFAESPYFNKNSNLLKAVKYLK
                     KISSEFDGDKLNDEKIWTAVFGKKEFNYGVMKNLTHELKKLTDKFTSIEIYSKNDLHI
                     NFNILNYLKYKSLPEMFEKTFVNTLKKYADEKLTFDISLYRYKAMDIERTFRVYFGKP
                     GSHKDLSGEKCMDELSFYFFSAYFYNSYCEIVDSSVYNFKSDSTNIRIFTDLYRENFY
                     GKNLLADIFYNSVMMFIESNGFGYFLELKKLFYENFNKLHIDIGYNIGAILTTYCVLS
                     KPVPGKHFVQEEFEILCFLFKNNILKHSSHKYIDSNLFSKFADICVVLDKPGQCEELI
                     EKCRNQLNPVNGKIRIQIADAHLQNHLKNYVKAIDIISRSKPVNCDEKLKLRGIELIV
                     NFNLKNYETVYTLIRNFRSFVEYEKILTETHREIFINFLNYLKRLTDLNINLYDPFKI
                     ENELSLMLKKISSEKTANRKWLTSVMNEMYEKTKKNSSKKKKI"
     gene            complement(16033..19755)
                     /locus_tag="PLX80_04945"
     CDS             complement(16033..19755)
                     /locus_tag="PLX80_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20176.1"
                     /translation="MKLIISALILILQFHSFTYSQISSKNNQSNIYKNFIPETDAFTE
                     RLLSGLNAGDSLGKSVSSAGDVNGDGYGDLIVGLPGYNSGAGRAYIYFGGLVFNSAPD
                     LTITGSAGNNLGSSVAGPGDVNGDGYDDVIIGLPGFSSGAGRVSIYFGGASMNNGSDI
                     NFGGESAGNQFGASVNGGGDINGDGFPDVIVGAPAYNSNTGRAYVFYCGVIINSTPDL
                     KMTGAATGNFFGFSVNLNGDQNADGFADAFVGAYGFSSNAGGVYLYKGGNIADTVQDL
                     FMNGGASSYFGYSVTNGGDINNDGYDDMIIGAPGLSAFTGRGYGYFGGGILNSTLDLT
                     YLSENPGDGFGTAVSFAGDYDSDGYDDVIISAINFNTGFGRAYLYKGGEIPNEEPVKY
                     FNGDNTGDNFGTSLRCAGDLNGDGTSEIIAGAHRNDANGSSSGRAYVFFNSMTGEDMP
                     DLTYSLSTGANEQMGHSVSNAGDVNGDGFADIIAGIPNYSAGSLQGRALIYFGGNNSD
                     TIADVTLTGVSALDNFGISVSAAGDVNGDGFSDVIVGASGYNAGNKTGRAYIFFGGSS
                     MNSTADVTMTGGSVNDEFGISVSSAGDVNGDGYSDVIIGARGYNSNTGRANLYYGGAS
                     MNNTVDVSFVGFSTGEFYGNSVSGAGDVNGDGFSDVIAGAYGFQSGAGRIQIFYGSTS
                     MDSYSDVNISGSDLDFFGYSVSDAGDVNGDGYDDVIIGEYGYFVNTGKAYIYYGGMVF
                     NTTADVTLTGLSGSEGFGTNVSGAGDINGDGYSDVLVGAPDFGAGSTIGRAYAFYGGA
                     SMNNTLDNVYYGTGFSSFGNSVSSAGDMNSDGLSDVIIGAPGFNSQTGRVSCYFSTPP
                     KVIPRIVSAKDVQFDQGGKLLLKWMRSGFDSPEINVVTFYKVEKSNPPSVNGFSWEAI
                     ANISAGHNISYDYTVDMPNDSMNANNGVQYFRVTALTSDVSQYWRSNILYGYSVDNIS
                     PAAPLDLSALNNIISVNLNWSSNTEPDLHHYNVYRNGIEIGTTLSSDFDDANVFDDSV
                     YNYQVAAVDIHGNLSQLSNTANINFNNAGQINITAAIEGFYIPASSNMIAGDTLIVYL
                     RNSAVPYSIADSFKGFINGNTLSGNFKFYNAPSGNYYIALKHRNSVETWSAAPHSYTY
                     HSNTNYDFSNLITKAYGSNQIQVDAAPLRFAVYSGDVNQDGIIDLSDGSLIDNDAFNF
                     SSGYLPTDVNGDGIIDVSDAVFADNNGFNFVGKITP"
     gene            20080..>20405
                     /locus_tag="PLX80_04950"
     CDS             20080..>20405
                     /locus_tag="PLX80_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20177.1"
                     /translation="MYGNNLTELLRTFSVNETKEFREFLNSSYFNKRQAVNNLYDIII
                     KFHPEFDNESLSKQIIFEKLFPGKKYNDTSLRVLTHYLTDLAQKYLGYKRFEKNKLEF
                     KIQLNH"
BASE COUNT         6357 a         4028 c         3367 g         6653 t
ORIGIN      
        1 ttcccagaca gttttattaa tttaaatcaa tcattgtatg atctgtcgtt tactcacttt
       61 catatttata ttattaataa cctttcagtc ggtaaaatta tcatattcgc aaagttcctg
      121 gttctggcag cagcctttgc ctgccggaaa tcagcttaaa ggaatctact ttgtcacacc
      181 tgagattggc tacgctgttg gagtcttagg tactattgta aagacaagca atggcggtac
      241 taactggcag actctggttt caaatgtgac agttgaattg gccggagttt attttattaa
      301 tgaaaataca ggtatggcag taggtgatag cggaacaata atcaggactt ctaatggagg
      361 tgataattgg aatatcatac caagcacagc acaatatctt ctgcaagatg ttatgttccc
      421 gtcggatctt gtcggttata tcaccggcgg tgaaggaata ttcaaaacaa caaacggcgg
      481 aatcaactgg gtaatgcaga caaattacgg aggaacatct gttaatttta caaatgactt
      541 gtttggtgct gtaggaggaa cacaaacact tgtttatact acaaatggcg gtgtgaactg
      601 gataaattca gaaatcaatt ttagtttctt agatcaggtt attggtgttt cacaaacaga
      661 tacaaataca ataatagcgg taacaaattc atcggacaga gttttaaaaa caacaaatac
      721 aggtatgaac tggattgaat attccttcgg tcctacgtta aatgatatac cacgggaaac
      781 atcttttgta aattccaaca caggatatat ggtaacagat ttcgggaaga tttataaaac
      841 agtaaacggc ggaattaact ggacgattga ttcaggattt gagacaagtt acagaaggca
      901 gggagtgttc tgggatatat gcacacataa ttccagtata gtaggaataa caggcggcgg
      961 cggcacaata atcaggagca caaattcagg aggtaactgg actgttcaga ccggtaattt
     1021 acgaacaata aaaggaagtt tttttataaa cgaaagtaca ggttataatg taggatacga
     1081 aggaacaatt cttaaaacaa ctaataaagg aactaactgg atcactcaat caagcggtgt
     1141 aacatctcat cttaactctg tgttttttac tgatttaaat accggatatg ctgccggaaa
     1201 tgaaggcagg atcttaaaga caactaatgc cggaagtaat tggactatgc aattcagcgg
     1261 aatgatgaac aatattaatt caattacatt tgtaaatgac agtttcggaa tttgcgctgc
     1321 tgacagcgga aagatagtaa aaacacttaa cggcggatca aattggttac cggaatatat
     1381 attaccacaa cagcatcaat tgttcagtgt tgcttttatg gacatgaata ccggaattgt
     1441 tacaggggaa aacggaagtt caatacgaac gaccaatggc ggattatcat ggtcttacaa
     1501 tgttatttca tttaatactt tatacaatgt ctgctatgca gattcttcaa ttttttttac
     1561 tgttggaact aacggtgcaa ttcataagtc atctaacgcc gggattaact ggacacttca
     1621 gtccggtaat tcaacagcat cactgaattc gataaaattt ttaaacacta caaacggatt
     1681 tgctgcagga agggatggaa cagttattaa aactactaac ggaggagtga gttgggtaaa
     1741 tcaaacaaag atcactaact atgaattata tgctgtttca atattaaata ataatgaagc
     1801 tataatagcg ggaaattacg gggtaacttt aaaaaccaca aacgggggtt tggtttttac
     1861 aaataaccag tacacattag ttaaagacga ctatagaatc aattccgtat atcctaatcc
     1921 gtttaacccg tctgcaaaaa taaattacag tatctcaaaa acaagcgatg tttcaataaa
     1981 gatctttgat atcaatggaa gggaaattac tgagcttgtc aattccatac aatccaatgg
     2041 ttcttatgaa atatctttgg atggaagtaa aaataatctt tccacaggta tatattttgt
     2101 ttctttgata gtaaacggaa atttaatgga ttctaaaaag ataatattaa tgaaataata
     2161 aagtggtatt gcgggcagag tttattctta ccagccggaa gcgggaggtt ttgcggagtt
     2221 gaaacggatg atcttattaa gtaaatttac aaactatgat acatccattt agttttttga
     2281 tatatggtcg ttgtttgttt tttttcggaa gcgagtgagt tttttactgc aatttgtaat
     2341 ttaaattaaa atagttttac taaaattaat attttataaa ttaaatttaa ctgccatgaa
     2401 aaaatctatt tttactttcg tgtttatttt tctgtttatc ctaacgaccg gtatttcata
     2461 ttcaattcaa ggctggtatg aattgccgca gatttctgca aacacaatga acagcattta
     2521 ctttatagat gaaaacaccg gatttgcttg tggaaataac ggttcacttt ttaaaactac
     2581 tgacggagca ttgacctggc agccggcagg cagtccgggt aattttgaat acagtaatat
     2641 tttttttact gataatcaaa aaggaattct taccactgtt cccatgggtt cggaggcagg
     2701 atattggttc agaatgtttg taacatctga tcaaggggta agctggacag agacacagca
     2761 gtattacaat tttatttctg atctatataa tgataattcc ggattttatt tttcgattca
     2821 ggggttttca aattttgaga ctcttggagg tctttttcat acacagaata attctatgaa
     2881 ttttgtttct attccttttt tcaattattt cagaaactca agtgtatttt ctgtctgcag
     2941 atctaacaat agaacctgga tctcagctta ttacggagat gatgtaggca ctgatgttaa
     3001 cagacttgga tatacagccg ataatggcag cagctggaat attgtactca atgactccgg
     3061 agtagcctgg aagcaagatc caatgcataa taaattttat aagatcagat ttattaatga
     3121 atccatcggc tttttgtctt caaaacttgg tttgatgaaa acactaaacg gcggagataa
     3181 ctggtctttc atagatacta caattacaaa aggtattcag acacatttct ttacatcagc
     3241 agacacggga tgggtttaca gattaaacaa tatttataaa acaacaaatg gaggtgaaaa
     3301 ttttgaactg cagtttattt ataattcgta tctcactcag ccgtttttta taaattcact
     3361 caccggatat attctttgca gcaatcaaaa aattcttaag actattacgg gtggtttgac
     3421 cgatgttcaa aatgcgaaca gtcaaaacat tccaaacact ttttcacttt cacagaatta
     3481 tccaaatcca ttcaacccaa aatcagttat caattacgaa ttgcaattta cgggccttgc
     3541 aaagttaaaa gtgtatgatg tgctggggaa tgaagttgcg gttcttgtaa atgaaaagaa
     3601 aaatgcggga tcgtattctg tagagtttga cggcaatggt tttgcaagcg gagtttattt
     3661 ttataagctg gaggcgggag attttgctga gacgaagcgg atgatacttt tgaaataaat
     3721 ttcattatga ttgttagttt attaaaagcc gggtttaatt aatgatccgg ctttttttat
     3781 tgaaaatatt caatgttgtt ttttgttcag ttcggatttg taatttataa aacttcacgt
     3841 gcaaactttt tagtcaccat caacacacac ttgactggct aacaaacatc ttcgggcatt
     3901 caattctgca cttttatttt ttaaaatgta tatcaaaatt gatcaagttt aaaatataga
     3961 attcagcatt agaaatgctg ccggtaaaat atttttgttg tctttagaat tttagacagc
     4021 atatcatatt taatgtaatg ataaaaaata tctgccgtga tattttataa atgttatttc
     4081 agtaacagca tactttttgt ttctgtaaat cctactgtct caagccggta aaaatatatt
     4141 cctcccgaaa gatctgaagc gtcaaactct gcttcataac ttcccgcaga aagtttttta
     4201 tttactaaaa cagcagcctg ctttcctaag atatcataca ctgataatct gacatcatgc
     4261 gcgccggttt tgtcatcatt cattatatca aaacggatct ttgtagatgg gttgaatgga
     4321 ttcggataat tctgtgaaag tgaatatgcc gaaggagttt catttgaaac tgataaaaca
     4381 tttgtgataa cggaaaattt aacaactgaa aaatccattc cggaagttac tcctgaaata
     4441 aatccgctga ctattatatt accggaatta tcgagagaaa tattattggc aacgtcattt
     4501 ctgtcagctg ctgagttgaa tctgtttacc caaacttcat ttcctgagta attatagttt
     4561 ataattacca tatcatccat tgtacctgtt ccctgactca gtccggcaac atagacattt
     4621 ccgttattgt ctgcggcaat acttcttggt tcatcaaaat tgtttgagcc cggtccgtta
     4681 taattttttg accatataag atcgcctccg ggggaataca ttatagtcat gtaatcatat
     4741 gaggtagaaa aacctgttga ggtaccggta acaaatatgt taccgagatt gtcacttgta
     4801 atggctttcc cttcttcaat attcacagtt gaagttgtaa accttctctc ccatatcaca
     4861 tcacctgcgg gagaatattt tattgttgca atatccagac cggtgcctgc tccaacgctt
     4921 gttccggtaa tgactgcatt tccggaaaca tcagcagtaa ttgaggtcat attatcattt
     4981 ccgtttgcag taccgttata agttttatgc caaagctcat caccgccggc gctgtatttt
     5041 ataattaaat aatcattgat cgtagctgct tcaaatgtac tgccgcatac aaaaatattc
     5101 ccggatttgt caacagccat atcatctgca aaatcattac cgcttgacgc accgttaaaa
     5161 tgctttttcc agagtagatc tccgtctgaa ttatatttga tcgtcacaac atcaaataat
     5221 acgccgggct catgactggt ccctgtaata attacattac ccgcttcatc agttactgct
     5281 ttttcggggg tctcagcact gctgctgttt ccgttgtatc ttctattcca ttgttcagcg
     5341 cccagatcat tatactttat caacacaaaa tctttgtccg tgccggatcc catactttct
     5401 cctgcgacaa aaacattacc cgtattatca agctctatgg aataagcaat atcatttccg
     5461 tttccgggac cgttgtaaat tctttcccac aggatatttc ccgacggatt gtatttcact
     5521 gtataaatat caaatcctga acctgaaccg aggatcttgc ctgaggaata aacatttccc
     5581 gaattatccg ttacatgcgt cagcgcttcg tcgttaccgt tcacgggacc gttaaaactc
     5641 ctgacccatt cctcattcag ctgtgatact gccgaatcgt aaattaaaat aaaaaataaa
     5701 ataataattg ctttcataat tttaaaattt gatttttaat ataaataaat caaacaagat
     5761 atctgatgtt tcactaaact aataaaaaca atttgctttt caaaacaaaa ttttcactat
     5821 ctgtagcttt catattattt tcaaaattca ggacaaataa aaatttaacc aatgcagtta
     5881 aagatatctt aatttaaaat taaaatgacg aattacagat gttataaatt atctgtaacc
     5941 catcatttgt aaaccgtcat tcgtaactcg taactactct tattaaattt taaattttaa
     6001 attttaaata ttaaatatta aatattaaat attaactatt aactattaac tattaactat
     6061 taactattat ggtgttattt tccccacaaa gttaaacccg ttattatccg catacaccgc
     6121 atctgataca tcaataatcc catcaccgtt cacatccgtc ggcagatatc ctgaattgaa
     6181 attaaacgcg tcgttatcaa tcagacttcc gtcggtcaga tcgatcgtcc cgtcctgatt
     6241 tacatcgccg ctgtatactg catatacaac cggcgcattg tccgcctgta tcatattatc
     6301 accatatgcc tgagtatttg ctgtcgagaa attataagtc atagagtttg aagtgaactg
     6361 ctgcgcagtc ttgctccaag tctctattga attcctgtgc ttaaaaacta aatagtaatt
     6421 tactgaatta actgcatttg aaaaaataaa agcgcttcct cctgtattgc ttaaaataga
     6481 tactgctgaa tctaccttcg aataaggaga cgcattattt cgtaaataaa ctcttaccgt
     6541 atcctgaatc ataagatcag atgaagaatt ataaaaaccc tgtatcagtg cagtaagatt
     6601 aagagtgctg atatcagaaa aatccgaagg agagtatttt atgattgcac tttgcgatgt
     6661 aggaatagaa cggtaacccg ctgcaaatac attgccggcg ttgtcaatat tgattgaatt
     6721 tcctctttca ccataagctg aatcaccata aattttcata ttcactgtag ttcctgtcga
     6781 attgtatttc attgtaagca tatcataagt attggacaag taattattac tgcaaaatcc
     6841 tataatgtaa atgttttcgt aaatatcagt tgtaatatca ctggggctat aatgagtata
     6901 atgaggatta tcataaatgt gagaccattg caaagccagg tctgaatcat attttacagt
     6961 gaaagtttta ttgttaaata ctcctgtgat taatatttga cctccgttgg atatggtcat
     7021 tgaatatgga taatattcct gggtaaaact tccgttgaga taagtttccc attgcagtat
     7081 gcctgatgtg ttatattttt ttgttaataa tttattatca ttacctgtta cataaagatt
     7141 cccgctgttt ccaataacta attctttaat gacataataa gcgtcattta ctgaccattg
     7201 aagagttcct gaagaattta gttttgtaat tactcccagt gctggattaa acgatacact
     7261 gttatcatac gctaagtaaa tatcatcagt actgttgcaa accattgctc ttgcatatac
     7321 ataattatcg tttcctattt tccgaaccca ttgcaaattt ccgttagaat tatatttcag
     7381 gattttaata aaattatcat attccgttgg atgagtactg ttgccaatta ttattggatt
     7441 acctgaatta tcaatttcca gatctacaat ctcatctgta tgatttaaag tatcatatgt
     7501 tcttacccat tcacgctgcc cgtctgaaag atattttatc agaaccagat ctttaccggt
     7561 cgcagagccg gttgcattac ctgcaacata taaatttccg taatcattaa ttatgatttt
     7621 taaaccttca tcatgtccgt ttcccgggcc attgtaagta tttgaccagt ataaataacc
     7681 tgacgaactg tatttctgtg tgagaatgtc cctgttcgaa gaaccggtat atccggttat
     7741 aaaaatatta ccgcagtcat ctgtaacact ttgatttgat gctgattgat tttcatataa
     7801 atttacccac gaagaatttt cgatgttaca ggatgcagga ataccaccgc cgttagttgt
     7861 cttaataagt aagccgcccg aaccgcacat ccatcccgtt gcgctgttta tgaaagatat
     7921 accaaaaaac ccctcaccat ttttgtagtt ataatctatt gcccagttat ctccgccgtc
     7981 atcagttttg gctttgaaaa atacttcacc gcatatatat cctgtctccg ggtcaggaaa
     8041 agagatctga ttaatgcctt ttggaaatga ttttaccgtc cacgacgtac cgccgtttgt
     8101 tgtccgcgca agattgttgt tggaagctcc ggcgtatcct gtagaggagt taatgaattt
     8161 tacatcattg aaagttgcag atgaaaaaga tgagttactc aaagatacat tatagcttga
     8221 accaaaattc gaagatttat aaacgtttgc atttgttaaa aaatttgaac ttgtgcttgc
     8281 aaaaacagta ttggcatctg ccatgtcaat tccaaaaatg ctcacaatcg gacttacgcc
     8341 gttcatattg ctgtttgtcc agtttaatcc tgaattagtt gttttgaatt gtctcctcga
     8401 ttcattggcg cagatacctg tcgatgcatt tataaaatcc atagcatgga tattaacagt
     8461 gtaacttgaa gtgttggtat tgttccattt gctacctccg tttgttgtct tcagtatctt
     8521 tccggaatca ccgcatatgt atcccgtatt tgcatcaaac attattatct ttctcagcgt
     8581 ctttgatgaa ttaatgtttt gggagatcca gcttgtaccg ccatttgttg tcttttgtat
     8641 tttaccgctt gtcccggcta tccagcctgt attaacattg ataaactgaa ctgcatacaa
     8701 atcagcattt gtgcctgttg atattgtatc ccactgcgcc gacaaatctg attctgaatt
     8761 tgtaaaaagt ataatggcaa cagtaaaaat tctgatgtat aactttttca tatttagtta
     8821 tttaattttg acaaatgtta tttgttattt caaagcgaat taattgacac ttaaaactct
     8881 tcaaatggaa ctcctcattc gcaactcaca actgttttat taactactaa ctcttaacta
     8941 ttaactatta actattaagg agttatcgcc accacaaaat taaaaccgtt attatccgca
     9001 tacacagcat ctgccacatc aactatatca tccccgttca catccgtcgg cagatatcct
     9061 gagttgaaat ttaaagcgtc gttatctatc agacttccgt ctgctatatc tataactccg
     9121 tcctgattta catcgccgct gtatactgcg tatacaaccg gcgcattgtc cgcctgtatc
     9181 agattatctc cgtatgcctg agtattaaat gtagagaaat tataactcag ctcagatgaa
     9241 gtaaactgct gtgctgtctt gctccatgtc tcgatggaat tcctgtgttg aagctgaata
     9301 tagtatccgg ttccgttcac agcatttgat aataaaaatg tacctgcacc tgtattgctc
     9361 agaatggaaa tggctgagtc agttattgca tagggcggcg aactgtttct cagatagact
     9421 cttgctgtgt cctgaaccat aaaatcagat gatgagtcat aaaatccctg tatcagcatt
     9481 gtcagattaa gattaacatc attactcttt ggtctgaatc ctccctgaga ttccgaactg
     9541 aagttgttat aatatctcca cggaccgaac ttctgaaatg ggtaagcagt cattgtatac
     9601 tgaagacggg tccgccactt atactgcttt tcagaattta atcctgtaat atcataagtg
     9661 aaatcacttc cggtaagtcc aagattttta aaagatgaca cactgttata ggatacgctg
     9721 tttgtaattt tacttcctcc tgtaaatgga attccatttt gctgaatgtc cgatactaat
     9781 cttactgtag ctttcccaaa aggactttta gcgaaatgac ttattctgac tttaccgttt
     9841 gaaccggtaa tgccatctga gcttacaata tcagatgttc cgtatttata ctggtttact
     9901 gtagaacgaa gagcttcact aattccattt ccaccataat aaacaaaagc cgatccttca
     9961 ttgatttgtc cattgctaaa tgtagcagca ccggttatca catcagaata accatctcca
    10021 ttgacatcgc cggcagatga tactgaatat cccaaattcg caaacgcaat aatgctttca
    10081 gcggtccagt ttgtggctga agataagcct gcagccgaac cgtgataaac atatactctc
    10141 ccttcatttt ccagattata atcataaaat ggagcgccta ctatcacatc agaataacca
    10201 tcaccattta catcaccggc tgatgatact gaattcccaa aatttgaatt tgcctgatta
    10261 ctttcagcgt tccaggatat aattgtggag agtcctgaag ctgatccgtt atagacaaag
    10321 gctctacctt catttgtatg tccgttatca tataaatttg ctccgattat cacatccgag
    10381 tatccgtctc cgtttacatc accggctgat gaaactgaaa acccaaaatt tgaatttgcc
    10441 tgattacttt caacactcca gtctgatgat aaggacaaac ctgagacgga tccataatgg
    10501 acaaaagccg cgccttcacc tgatattccg ttataataat tgttcgcccc aactatcaca
    10561 tcagaatatc catctccgtt tacatccccg gcgctcgaaa ctgaaaaccc atattgtgaa
    10621 ttcgcttggt taatttcact actccagctg aagcttaatg atattcctga accggatccg
    10681 tgatatacaa aggcagcgcc ttcatcggtt tgtccattat caaaatctct tgccccgact
    10741 attaaatcag aatatccgtc accatttaca tcgccggcgc ctgaaaccga taacccaaaa
    10801 agcgcactct cctgattgct ttcaccgctc cagcctgctg tggctgaaag tccagaagct
    10861 gatccgtgat aaacaaatgc tgctccttca tcagtaaatc cgttgtcgaa atatggagct
    10921 cccacaatta catcagaata cccatcacca ttcacatcac ctgcagagga tactgacgtt
    10981 ccaaaatatg cattcacctg atttccttcc aagctccagt ttgaaaatga agataatcct
    11041 gatgctgatc cgtgatagac aaaggcagct ccttcatctg tttgtccgtt atcaaatcta
    11101 cgagcgccta caatcacatc agaatatccg tctccgttaa catcgccggc tgatgaaacc
    11161 gaaaaaccaa aatctgaatt actctgatta ctttcagagc tccagacgaa agcgctgctc
    11221 atcccaattg ctgatcctaa gaaaacaaat gctctgcctt cattagcact tggactatca
    11281 aacatatgtg aaccgacaat tacatcacta aagccgtccc catttatgtc tcctgctgtc
    11341 gctacgctga ttccaaacct cgaattcgac tgattgcttt cgtatataat tgctgaaccg
    11401 gaacttaacc cgcctgaata tccaagataa acaaacgctc tgccttcatc tgtttgaccg
    11461 ttatcaagat aatttcctcc gagtattaca tctgcaaacc cgtcggcatt caaatcgcct
    11521 gctgtcgata cgctgattcc gaattgtccg ctgatttcat tgctctcttg tgtccatact
    11581 gcagcagctc caatacctga ggaagatcca agataacagt aacaccttcc ttcatcgacc
    11641 tgaccgttag tatacagtgg cgcgccgatc ataatatcag agtatccgtc accatttaca
    11701 tcaccggctg tgcttacact gtatccaaaa ttttcgccag tctgaagccc ggttcttgta
    11761 gttgtaggtg tagccggaaa ggtatttccg ttactgtgaa atatatacac tcttccgtag
    11821 tcaggaagtc cgatatcgta cctcggagct ccgattgtta tatcggaaaa attatctccg
    11881 ttaatgtctc cggctgtagc aacactgaat ccaagatatg tatctaaagt attcatttca
    11941 tatgtccagt ctgctgtaac aggcaatcct ccggtactac ctttaaatac ataaactctt
    12001 ccttcgtttg tctgaccatt atcataaaac ggacttccga ttaccacatc actgtaaccg
    12061 tcattattaa catcaccggc acaagctaag ctgtatccaa agttttcacc tgcctgattg
    12121 gtttcatatt tccaggcatg aaccgcctga agaccgctta cagaaccgta ataaacatat
    12181 gctctccctt catctgtctg cacgctgtca taagtcggcg aacctattat cacatcactg
    12241 tatccgtcac cgtttacatc accggctgta gctacactcc atccgaataa tgctgatgac
    12301 tgattggatt ccattgtcca gtttgctgat aaagataaac cgtttataga tccgtgataa
    12361 acaaatacac tgccttcatc tgtctgacca ttatcataat aaggagctcc gacaattaca
    12421 tcagaatatc catcatcgtt tacatccccg gcagttgcaa cgctccagcc gaaattcgcg
    12481 tttgcctgat ttatttcagc agtccaggct gcagtcgatg aaagcggatc tatagttact
    12541 ggatatgctg cgtcttcatc atttacaaca ataaggaatg aattatgaat tactttgctg
    12601 tcagatttat cttttaattc gtaattcgta attcctaatt cgtaattttt ttcaaaataa
    12661 gctcttagtt ctttaccatt tgcatcccag actttcaaag aagcatactg taattttata
    12721 tctcccttct catctttaaa cttcagcgca tctccgcctg tgagcatttt cagttttgta
    12781 tcggcattga tgatcagtct taacctgtca tttcccacgg gtttattctt tacaataaaa
    12841 tcctgacgca ttccgttttc atcatttgaa tattgaattc tgagattttc gttttctatg
    12901 aaaattttat ttccgcctgc tgacagttca atttcattaa aatccaggta atcatttcta
    12961 agatctgatt ttaaatcttt actcacgctt tgaaggtcga agtcaacttt ccagtcatca
    13021 agatatttat actttttatc tatctcttcg atcatcttat ccgactcatc aaacaaagga
    13081 atcttattac tacggagctt tgcgatgaat ccgcgtttgt tatatatgaa tctcatgttg
    13141 tttgcccggt tcggagactg atatgtattt gattcattat tatatgttac atcatactcg
    13201 ttcttttgta tatttgacat cgctgttgaa taccagtcgg atgtttcatt actctttacc
    13261 tcatttgatc tatcatgctg aacagatgaa actgacaagt caaatggaga gtcctgtgtc
    13321 agatcaaact ttaaatcaac tctctcataa gatctgtttt ctttgggaaa cgattttgaa
    13381 tccggtgaat tctttgttga catatatatc atcaacagag taagtgataa aataaatagg
    13441 atgagttttt tcataattag tttttgatta ttaatgaatt tgaccttaag atattttttt
    13501 gatttgtatt caattcgcaa ctgttttatt aactattaac tattaaaact tcctgtcagg
    13561 tttaacctcg aagaatgttt tacagcaata taacttgtgc ctgttccgtt aaatgaacaa
    13621 gcctgatccc agaatagctt agctgacgct tcacgggaat ttagtacaag tacgattaaa
    13681 aaaactgatg taagcagttg tattcttgtc ttcatttttt tttgtttaat ttattttaat
    13741 atatttttta attatctcag gtgaataatt agttacagat tgtcaggaag cctttaaccc
    13801 gccctcatga gcagcgggtt taatcggcgt tcttcctgag tgactatcgg gaagtaattg
    13861 agataatttt attataaaaa ataactgatg tcactcaggc atgttaaaat actttacaaa
    13921 cctaaatcaa taagcgcgga tatcaaaaca gaaaaaattc agtttttcac attgggtttt
    13981 taaagtggtt agccgggatt tttaatttcc ggtctgtttc gaaaggattt ttaaaatatg
    14041 ataaataatt tattcacctt aaaaatattt tttattgttg taaaatattt attatagttt
    14101 attatttaag gtcggactac aggatggtct aaatgaggtt ttgcgtttaa taatttctta
    14161 ccggatatgt ctgttaaatc tttttctttt tggaggaatt ctttttagtt ttttcataca
    14221 tctcattcat tactgacgtc agccattttc tgttggcggt tttttcagag ctgatctttt
    14281 ttagcattaa agatagctcg ttttctattt taaaaggatc gtaaagattg atattcaaat
    14341 cagttaatct cttcagataa ttaagaaagt ttataaaaat ttcacgatgc gtttcagtta
    14401 atattttttc atattctaca aaagatctga aatttcttat aagagtatat acagtttcat
    14461 aattctttag attaaaatta acaattaact caataccccg cagttttaat ttttcatcgc
    14521 agttaacagg ttttgatcgg gaaattatat caatagcttt aacatagttt ttaagatgat
    14581 tctgaagatg tgcgtcggct atctgaattc tgatttttcc gttaacggga ttgagctgat
    14641 ttctgcattt ctctataagc tcttcgcact gccctggttt atcaagcaca acgcagatat
    14701 cggcaaattt agaaaataaa ttgctgtcta tatatttatg actgctgtgc tttaagatat
    14761 tatttttaaa aagaaaacat aagatctcaa actcttcctg aacaaaatgt tttcccggga
    14821 caggttttga aagaacgcag tatgtagtca gtatagcccc gatattgtat cctatatcaa
    14881 tatgaagttt gttaaaattc tcataaaaca atttcttcag ctccaggaaa tatccaaaac
    14941 cgttactttc tataaacatc attacagaat tataaaaaat atcagcaagc agattctttc
    15001 cgtaaaaatt ttccctgtaa agatctgtaa agatcctgat atttgtagaa tcagatttaa
    15061 agttgtaaac agaggaatct acaatttcac aataagaatt gtaaaagtag gctgaaaaaa
    15121 aataaaaaga cagttcatcc atacactttt ctccgctaag atctttatgt gaaccgggtt
    15181 ttccaaaata gaccctgaat gttctttcaa tatccatcgc cttatacctg tataaggaaa
    15241 tgtcaaatgt taatttttca tctgcatact ttttcaatgt atttacgaaa gtcttttcga
    15301 acatttccgg aagggattta tactttaagt aattcagaat attgaaattt atatgcagat
    15361 catttttgga ataaatttct atggaagtaa atttatcagt aagttttttt aattcatgtg
    15421 tgagattttt cattactccg taattgaatt ctttcttacc gaatacggct gtccatattt
    15481 tttcatcgtt taatttatct ccgtcaaatt cagaagatat tttttttaaa tacttaaccg
    15541 ctttaagtaa atttgaattt ttattgaaat atggtgactc tgcaaattca acaaatcttt
    15601 taaattcgtc cctgctgaat gtctttatta tttcaattgc cggagatttt atcattacta
    15661 aaattaaatt tcagtaataa aactaaatat tatttatttt acaattcagt ttttaaattt
    15721 ggaaagtatt gatgacacag attttcatat gtgaattgaa taaactatca cgcagtttga
    15781 aacacaatta aatactattt aaaatttcca ataaaaaaga gccctatgaa aaataaaaca
    15841 gactctggta taatcaaatt aaaaatgaca attttcaact tttaaattac agattgatac
    15901 ctatttaatt tatctgcaaa taaaatttga aacctgtcat tcctaactgc tcttattaaa
    15961 tattaaatat taaatattaa atattaaata ttaaatatta aatattaact attaactatt
    16021 aactattaac tattaaggtg ttattttccc cacaaaatta aatccattat tatccgcaaa
    16081 caccgcatct gatacatcaa taataccatc accgttcaca tccgtcggca gatatcctga
    16141 actgaaatta aaagcatcat tatcaataag acttccgtca ctcagatcta taataccgtc
    16201 ctgattcaca tcaccgctgt aaactgcaaa tcttagggga gctgcatcta cctgtatctg
    16261 attgcttccg taagctttcg ttatgagatt tgaaaaatca taatttgtat ttgaatgata
    16321 tgtataagaa tgcggcgcgg cgctccaggt ctcaactgaa tttctgtgct tcaatgcaat
    16381 ataataattt cctgaaggag cattataaaa tttgaaatta ccggataagg tatttccgtt
    16441 aatgaatcct ttgaatgagt cagcaatcga atatggtact gcagaatttc tcagatacac
    16501 tattaaagta tcgcctgcga tcatattgct ggaagccgga atgtaaaacc cttctatcgc
    16561 tgctgtgata ttgatctgac ctgcattatt gaagtttatg tttgctgtat ttgaaagctg
    16621 acttaaattt ccgtgaatgt ccacagctgc aacctgataa ttataaaccg aatcatcaaa
    16681 aacatttgca tcatcaaaat cagagctcag tgtagttccg atttcaattc catttctgta
    16741 aacattgtaa tgatgaagat cgggttcggt attagaagac cagttaagat ttacactgat
    16801 aatgttatta agcgctgaca gatccagagg tgcagcggga gaaatattat ccacactgta
    16861 accatacagt atatttgaac gccagtattg actgacatcg gaagttaacg cagtaacccg
    16921 gaaatactgc acgccgttat tagcgttcat tgaatcattt ggcatatcga cggtataatc
    16981 ataggaaata ttgtgacctg ccgaaatgtt tgcaattgct tcccaggaaa atccgttaac
    17041 cgaaggagga ttgctttttt ctactttata aaaagttaca acatttattt ccggtgagtc
    17101 aaatccgctt cgcatccatt ttaacaaaag ctttccaccc tgatcaaact gaacatcttt
    17161 tgcagaaaca attcttggga tcactttcgg cggtgttgag aaataacatg aaactctgcc
    17221 tgtctgtgaa ttaaatcccg gtgctcctat tattacgtca gacaagccgt ctgaattcat
    17281 atctccggca gaagaaacag aattaccaaa ggaactgaaa ccggttccgt agtaaacatt
    17341 gtctaaagta ttattcatcg aagctcctcc ataaaatgca tatgctcttc cgatcgtgct
    17401 tcctgctcca aaatccggcg cgccgactaa tacatccgaa taaccgtcac cattaatatc
    17461 tcctgcacct gaaacgtttg taccaaaccc ttccgaacct gacagacctg tcaacgttac
    17521 atcagctgta gtattgaaaa ccatccctcc gtaatatatg taagccttgc cggtatttac
    17581 aaaatatccg tattcgccta ttattacatc atcatatcca tcaccgttca catcaccggc
    17641 gtcagaaaca gaatatccaa aaaaatccaa atcggatccg gagatattca catcagagta
    17701 actatccatt gaagttgatc cgtaaaaaat ctggattctg ccggctcctg attgaaatcc
    17761 ataagcgcct gcaatgacat cagaaaaacc atcaccgttc acatcaccgg ctccggaaac
    17821 cgagttgccg taaaattcac ctgtcgaaaa tccgacgaaa gaaacatcaa cagtattatt
    17881 cattgacgcg cctccataat aaagatttgc tctgcccgta ttagagttgt atcctcttgc
    17941 gccaataatt acgtcagaat acccgtcacc gtttacatct cctgctgatg atacagatat
    18001 tccaaactca tcattcactg atccgcctgt catcgtaaca tcagctgtac tgttcattga
    18061 acttccgcca aaaaaaatat aagcccgtcc ggttttattg ccggcattat atccggaagc
    18121 gccgacaatt acatctgaaa atccgtctcc gtttacatca cccgctgcag aaacagaaat
    18181 tccaaagttg tcaagagctg aaactcctgt aagcgttaca tccgcaattg tatcagaatt
    18241 atttccgcca aaataaatta aagctcttcc ctgaagtgac ccggcggaat agtttggaat
    18301 tccggctatt atatccgcaa acccatcccc gtttacatct ccggcattag aaacagaatg
    18361 tcccatctgt tcattagcgc cggtcgataa tgaatatgta agatcaggca tatcctcacc
    18421 ggtcattgaa ttaaagaaaa cataagccct tccgctggat gaaccgttag catcatttct
    18481 gtgcgctccg gctataattt cagaagtgcc atctccgttt aagtctccgg cgcatctcag
    18541 cgaagtaccg aaattatcac cggtgttatc tccgttaaaa tatttaaccg gttcctcatt
    18601 tggtatttct cctcctttat ataaatatgc tctgccaaat cccgtattaa aattaattgc
    18661 tgaaatgatc acatcatcat atccgtcact gtcataatca ccagcaaaag aaactgctgt
    18721 tccaaacccg tctcccggat tttcactaag ataagtcaga tcaagagtac tattgagaat
    18781 cccgccgcca aagtaaccat atcctctgcc tgtaaaagct gataacccgg gtgcgccaat
    18841 gatcatatcg tcatatccgt cattattaat atcgcctccg ttagtaactg aatatccgaa
    18901 atagctggat gcgccgccat tcataaaaag atcctgaaca gtatcggcaa tatttcctcc
    18961 tttatataaa tatactcccc ctgcatttga gctaaaaccg tatgctccga caaaagcatc
    19021 agcaaatccg tcagcgtttt gatcgccgtt taaattcaca gagaaaccaa aaaaattacc
    19081 tgttgcagca cctgtcattt tgagatcagg agttgaattt ataattactc cgcaatagaa
    19141 aacataagct ctgccggtat tggaattgta agccggtgcg ccgactatca catcaggaaa
    19201 accatcaccg ttaatatcgc cgccgccgtt tacagaagca ccaaattgat tccctgctga
    19261 ttctcctcca aaattaatat ctgaaccatt gttcattgat gctccgccga aataaatgct
    19321 cactcttcct gctccggaac taaatcccgg aagacctata attacatcat catacccgtc
    19381 accatttaca tcacccggtc cggctacaga tgaaccgaga ttatttcctg cagaacctgt
    19441 aatcgtaaga tcaggagcag aattaaaaac caatcctccg aaataaatat atgctctgcc
    19501 cgcgccggag ttatatcccg gaagcccgac aattaaatca ccatacccgt ctccgttcac
    19561 atcgcctgcg ctgcttacag atttaccaag actgtcaccg gcgttaagtc cgcttaaaag
    19621 ccgttctgta aaagcatcag tttccggaat aaaattttta taaatgttgc tttgattatt
    19681 tttactgctg atctgtgaat aagtaaatga atgaaactgt agaattaaaa taagtgctga
    19741 aatgattaat ttcattatga ggattttaag tttttaaaca gttataaaat attagttaat
    19801 agttagtagt taatagttag tagttagtag ttaattgtta attagggttt ttgattttga
    19861 aaaatttacc ctggctgtaa tgaagatctg ctttgatatt tacattaaaa tctcagcaaa
    19921 aatatgattg ttttagttat gtgacaagta atagtttttc aaattgttac acggacttcc
    19981 gcatgattta attaaaatac ttccaaaaca agctgttttt aataagttta ttaaaaaaaa
    20041 tataatctat atgttacaga aaattttcca tttaatttta tgtatggtaa taacttaacg
    20101 gaattactga gaacattttc cgtaaatgag acaaaggaat tccgtgaatt tcttaactca
    20161 tcttatttta ataaacggca ggcggttaat aatttatatg atatcataat taaatttcat
    20221 ccggagtttg ataatgaatc tttatcaaag caaattattt ttgagaaact ttttccaggt
    20281 aagaaataca atgacacttc actcagagta ttaactcatt atctgaccga ccttgctcaa
    20341 aaatatctcg gatataaaag atttgagaaa aataaacttg aattcaaaat tcagctgaat
    20401 catga
//
LOCUS       DAORVQ010000038        20340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1041_length_20340_cov_4.918610, whole genome shotgun sequence.
ACCESSION   DAORVQ010000038 DAORVQ010000000
VERSION     DAORVQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 20340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20340
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1041_length_20340_cov_4.918610"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..2749)
                     /gene="alaS"
                     /locus_tag="PLX80_04955"
     CDS             complement(50..2749)
                     /gene="alaS"
                     /locus_tag="PLX80_04955"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQY20178.1"
                     /translation="MKSKEVRQSFLDFFKGKEHTIVPSSPVVPQNDPTLLFTNAGMNQ
                     FKDVFLGSGKRDYTRAADTQKCIRAGGKHNDLEEVGADGYHHTFFEMLGNWSFGDYYK
                     KEAIFWAWELLTDVWKLDKKRLWISVFETDEEAFDIWKNETDVDPSHILRFGEKDNFW
                     EMGDTGPCGPCSEIHYDSTDNGCKPEDVNAGLEEVVEIWNLVFIQYNRKKDRELELLP
                     KKHVDTGMGFERIVRVLQNKKSNYETDVFLPIINKICEITKKEYSGSSIPSINAIADH
                     IRALTFAISDGAIPSNEGRGYVLRRILRRASNHASKLDYHEPILFNLVDTLIENFGEV
                     FPEIVSKKDFVKEVIKAEEESFHLTLERGIKLFQEIANSLKEKNTKIFPGDQAFKLYD
                     TYGFPLDLTQVMAKELGLKIDLLVFEEEMEKQKERGKSSRKEIVYEAPELLDDTANKF
                     DTEYDPYKFSGKDFFTEMVIPDREKNLIVLKNNPFFSESGGQISDTGFIETDEGFKIN
                     IIDSKKNFLISENSDLNNFKDGRVKIILDLERRRSIERNHSATHILHEALRRILGEHI
                     KQLGSLVSDEYLRFDFPHFHKVTDEQIHEIEDLVNSKVNEEIEIYTDVDISIEQANKI
                     PNVKKLFGEKYGDKVRVVHIDDNFSVEFCGGTHLKNTSDIGLFKITKEESVAAGTRRI
                     FARTGSGILHFLNERISDIDKIFHELPISYSDKVMNSLEKFKNEFQSADFKDAALMKR
                     LLNFQDESFTSLIELREKYSEEKKLADKELLKQNLQILFGEIDEIISNSKEVNGYKLL
                     AEKINAGNADEFREIGDYLRNKVTNGITVIASVIDDKVNIVCTVSDNLIKEKGFNAGK
                     IISVIAKELGGGGGGKPHLATAGARDIEKLPEVLSKVESYLNN"
     gene            complement(3279..3695)
                     /locus_tag="PLX80_04960"
     CDS             complement(3279..3695)
                     /locus_tag="PLX80_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HQY20179.1"
                     /translation="MDKSENRSAELKISSNPALRILLLISGLLLTAIGILGIFLPLLP
                     ATIFFILAAWCFARSSEKFHGWLHNNRFFGKYLSNYTSGNGMTLNSKIMSIAMLWIGM
                     IISAIWLTELIYVRILLALIGIGVTWHLLAIKTAPK"
     gene            3879..5354
                     /locus_tag="PLX80_04965"
     CDS             3879..5354
                     /locus_tag="PLX80_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY20180.1"
                     /translation="MYTKLKNALVFANSNAYFYKIFYLINDLYIMKKYFLSIFAVLFI
                     ANLSYSQLPNQNTYLFAQVDQYQSYSALWGYKAPDSREYAILGCNTGTSFIDITDSGN
                     VRQVDFVTGITNNWREMKTYSHYAYVVSEATNSGLQIIDLQYLPDSVVLVKTWSYAGY
                     TKTHSIQQSGSYLYLNGGNSAANGGVTVVDISDPVNPVKMGQWTTEYVHDCRILNDTI
                     WASNIYTGETSIINATNKNSLDFVRTWRSYPTSEVSTHNSAFTTDRKYCYTTNEISSP
                     NGRLNIWNIEDVNNITFIREWLPTGISTSIVHNVEIYGNLAVIAHYRAGIRILDITNG
                     SNPTEVAWYDTYPQSNSASYSGCWGVYMFPSGKIIGSDMSGGLIVIKTLNVLTGVSNN
                     YVNSIPEKFSLDQNFPNPFNPSTTIKFNLKDNSKIKLSLYDIAGIKIADLINDNRDAG
                     SYEFNFDAARYNLSSGTYFYSLEGNDINGNFTDTKKMILIK"
     gene            5374..6762
                     /locus_tag="PLX80_04970"
     CDS             5374..6762
                     /locus_tag="PLX80_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY20181.1"
                     /translation="MRKLTVLFILVVIFLVSSNANSQLPNQSTYLLKNLNQHSVTQFQ
                     YSAVWGYVAPDGREYGILGCPGGTAFIDVTDSANIREVDFVPSLTNNNWREMKTYSHY
                     AYVVSEATNSKLQIIDLQYLPDSVSLVNTWSYPTYTKTHSISQFDHYLYLNGGNSASS
                     GGITILDVIDPVNPVKLGQWTDLYVHDCRVVNDTIYAANINDERVSIINATNKSAPST
                     VRTFVNLPGSGPHNTALSKDRKRLFVTDEIGTAPYRLKVWNIDDVNNITYITSWNPTN
                     ITNSIVHNIETYNDIAIVAHYSAGIRILNISNPDAPQEIAWYDTYPLNNNESYNGCWG
                     VYMFPSGKIAASDRQTGFYCIKSTEVLTGISSNISASVPENFSLNQNYPNPFNPSTKI
                     NFSLPKYSQASLKIYNVNGKEVAELFNDTRDAGNYEVSFDAAKYGLASGTYFYNLKTQ
                     DFNETKKMTILK"
     gene            6962..8710
                     /locus_tag="PLX80_04975"
     CDS             6962..8710
                     /locus_tag="PLX80_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20182.1"
                     /translation="MKTLILLFTIIFIPEISFPQTFFEKETTGAIVQNFNFNHIMAWG
                     DYDNDNDQDVMINGLNDQCSTCYMPIIFLRNEGNGNFIRIYDNPIAQTFITGSGMAWG
                     DYDNDGWLDLFICATLNARNRLFHNDGNGNFTEILTGVIVTEMNSSQSCNWVDYNKDG
                     WLDLYVTNRYGIYSNRLYKNNGDGTFTKILSGSIVNDIGESRGSTWGDYDNDGWPDLF
                     VVNFGGQNDFLYHNNGNGTFTRIFNLPMVNYAAWGSSCHWVDFDNNGWIDLFVTNNNS
                     ANRLYYNHGDGNFTLSNSLLSQKVNGLGFTWGDYDNDGLTDLFLCDWYGTNTLYKNQG
                     GSMFVRVSNEIPSQEGLHSSTAAFTDIDLDGKLDLLVANRLDNPFNYQYNNIGNTGNY
                     LTIQLKGHASNKNGVGAKIKIVIGNTPQYKELLLGSNGHNSGSTLWPHFGIGNNQGVD
                     SIIVNWPSGAVTRSGYVNGNRIVIIDEDFGVIGINEENITAVQYNLYQNFPNPFNPST
                     KIKFEIAKNSFTELSIYDVNGRKIETLVNEFKRPGSYEIEFNSNNSVRNLSSGIYFYK
                     LSAGDYVSTQKMILIK"
     gene            8924..9274
                     /locus_tag="PLX80_04980"
     CDS             8924..9274
                     /locus_tag="PLX80_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20183.1"
                     /translation="MESITQKRPLTRREFKMKYYKLAMIGTMIFIFSITVGMLGFHFI
                     VKLNWVDSFLNSSMILGGMGQISELKTDAEKIFAGCYALFSGVTFLTSIAIFITPLLH
                     RVLSRHHIDLDSKE"
     gene            complement(9324..9560)
                     /locus_tag="PLX80_04985"
     CDS             complement(9324..9560)
                     /locus_tag="PLX80_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20184.1"
                     /translation="MKCTDKISLLIPFGKKNDRKFSHSTIPEFSFDLYADDIKATVEY
                     FETYKIKILKPINHEDKWTVISDPDGLANEVFQK"
     gene            complement(9830..10750)
                     /locus_tag="PLX80_04990"
     CDS             complement(9830..10750)
                     /locus_tag="PLX80_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20185.1"
                     /translation="MKKITLFFLLFAFSFNINAQGNYPKLQDVKSLSSLKFEKTDSPL
                     DKNLEKFNSEIPLNSLSLKKDLTNQSASKPKKFSFSIIPYVWFTSIGGTVGYGPNGEK
                     YGFNKSFSDAVKYLKMAAAVTGKIKYERVSFVYDISYLNLKGFGTEIPVSTPQILSSN
                     WTVKQAIYDLFLAYLFPSTSRTVRVDVYAGGRLWNLDVESTIIDNTSTVAKMGQYTNS
                     WFDPVIGINAEYLLSKDMKWAAWTKGDIGGFGVNSQMTWQMNAGFAYLLSPNVPLSLG
                     FKYVGVNYDKTARNWTVNEYGLTLGIGYRY"
     gene            11605..11949
                     /locus_tag="PLX80_04995"
     CDS             11605..11949
                     /locus_tag="PLX80_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY20186.1"
                     /translation="MKEFFKKNKLKILVFAVSILFVAYIVNGINEHTVIPDHIHSIDD
                     KASEEWTDEEKITVLKRTLQNEPDNIEAMLKLADLYIHTGDNKSAKKTIDKVLEIDPY
                     NKEATVLQSELK"
     gene            12364..12558
                     /locus_tag="PLX80_05000"
     CDS             12364..12558
                     /locus_tag="PLX80_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20187.1"
                     /translation="MEVPFTYFITKNNKLIISRNNKQIKTVKGKGFEDFKVLLKGGDE
                     DKIQLKLAKLTGYYKHGNEK"
     gene            complement(12568..13053)
                     /locus_tag="PLX80_05005"
     CDS             complement(12568..13053)
                     /locus_tag="PLX80_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HQY20188.1"
                     /translation="MELLYLLFGIYIISSIIIRVRKGEFDHSLSGRIAMSAMLLLTAS
                     GHFIFPEGMAKMIPDFIPFRIFLVYLTGILEFLAAIGLIIDRYSRITSLLLIIFFIAI
                     LPANINAAINNIDLRTGDNSGPGIEYLYFRIPMQLLFIGWTYLFGLKLNRKDLKEKAE
                     T"
     gene            13229..15379
                     /locus_tag="PLX80_05010"
     CDS             13229..15379
                     /locus_tag="PLX80_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HQY20189.1"
                     /translation="MSNLLLQHISNSLSISLKQVTATVDLLNENATIPFISRYRKEAT
                     GSLDEVQVSDISSELKRLTELIKRREYILKSISDQEKLTPGLEAKIIGCWDPIDLEDL
                     YLPFKPKRKTRAAAACEKGLEPLAKLIWSQENSDIFNEAKAFVNDDVVTIEDALEGAR
                     DIIAEWINEDADARNSLRNLFKHSAVITAKVVTKKKEEGQKYLDYFEYSEDFRKCPGH
                     RLLAIFRGEKEGILRVNAGVDTDLAVKILEKQFIKPDKINSVNSSAEQIVKAIIDSFK
                     RLLAPSLETEFRNLYKEKADKEAILVFTENLRQLLLAAPLGQKVTMAVDPGFRTGCKL
                     VCLDEQGSLLHSTTIYPHPPQNQMKESAEVLIKLIKKYNIKAIAVGNGTAGRETENFI
                     KGIVKEIKDTEVYLVNEAGASIYSASEAAREEFPELDLTVRGAISIGRRLMDPLAELV
                     KIDAKSIGVGQYQHDVDQQKLKESLDQTVISAVNLVGVNLNTSSQHLLQYVSGIGPKL
                     AKAITKYRNDKGPFSSREELMKVGGVGSKSFEQAAGFLRIPGAKNPLDNSAVHPESYT
                     IVQMMAKDLSLPVKDLIVANEIIKQIDIKKYVNDKVGIPTLEDIINELKRPGLDPRGE
                     TTVFNFADDINDIKDLKIEMRLPGIITNITKFGAFVNIGIKENGLLHISQISSNRFIK
                     DPAEVLKLDQKVIVRVVSIDEERKRIQLSMKEKE"
     gene            15452..15673
                     /locus_tag="PLX80_05015"
     CDS             15452..15673
                     /locus_tag="PLX80_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQY20190.1"
                     /translation="MILIVLYIYSSQINLKSKIKNLKYAIVIERSKTGFGAYVWDLPG
                     CASVAKTKPEIKELIKDGIEFHLEGMKLN"
     gene            complement(15959..17614)
                     /locus_tag="PLX80_05020"
     CDS             complement(15959..17614)
                     /locus_tag="PLX80_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQY20191.1"
                     /translation="MPKLKSGLKKDTLFEKREEFYKDLIKKTIALKKEIHFGGGKKAI
                     EKLHSKNKLHCRERVDLLIDKDSYFMELCIFAGYGMYKEYGGAPASGTIFGIGKIHGR
                     NCVIVANDATVKAGAWFPITCKKNLRAQEISIENKLPIIYLVDSAGVFLPMQDEIFPD
                     KEHFGRVFRNNAVMSSMGIPQIAAIMGPCVAGGAYLPIMSDEALIVEGTGSIFLAGSH
                     LVKAAIGEKIDNESLGGAAVTTEISGITDEKMADDKECIEKIRELVSKFGEMPKAGFD
                     RIKNKNPNHNEKEIYGVLPEDTAKPYDTYELIGRIIDDGDFDEYKPEYGKTIITGYAR
                     IEGWAVGIVANQRSIVKSKSGEMQFGGVIYSDSADKAARFIMNCNQRKIPLLFLQDVT
                     GFMVGSRSEHGGIIKDGAKMVNAVANSVVPKITIITGNSYGAGNYAMCGKAYDPRFIF
                     AYPNAQIAVMGGSQASNVLLDIKMGQLKKQGKELTEEEKKSFLEEIHSRYDEQTNVLY
                     AASRLWVDEVIDPKDTRKIVSYCIELANNNSEIKKFNVGVIQT"
     gene            complement(17716..18999)
                     /gene="clpX"
                     /locus_tag="PLX80_05025"
     CDS             complement(17716..18999)
                     /gene="clpX"
                     /locus_tag="PLX80_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQY20192.1"
                     /translation="MSTKSGSKNQIARCSFCGRNADKVTNMIKGPSDHNGKEVFICEI
                     CVGNAMQIIKKNTLSSATKSINKVRSDLTPQAIKKFLDEYVIGQDKAKKSLAVAVYNH
                     YKRIDSIDFNYIDDVEIEKSNILLIGPTGTGKTFLAQSLARLLDVPFAIADATTLTEA
                     GYVGDDVESILVHLLQNANFDLSKAERGIIYLDEIDKISRKSDSTSITRDVSGEGVQQ
                     ALLKLLEGTIAGVPPKGGRKHPEQALINISTKNILFICGGAFDGIDKIIEKRISKSSI
                     GFGSDIFSKDKSDQDLLTSQVEPDDLIHYGLIPELIGRLPVITTLNSLDRKTMLSILT
                     EPKNAITKQYQKLFRMEGVDLEFTEDALEGVVELALKRKTGARALRSILENIMLDIMY
                     KIPSKENVSNCIITKEVISEKKEPVYLSGNRKVIA"
     gene            complement(19043..19124)
                     /locus_tag="PLX80_05030"
     tRNA            complement(19043..19124)
                     /locus_tag="PLX80_05030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19088..19090),aa:Leu,seq:tag)
     gene            complement(19353..20192)
                     /locus_tag="PLX80_05035"
     CDS             complement(19353..20192)
                     /locus_tag="PLX80_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20193.1"
                     /translation="MKIYKALVFVIVAAFIFSIYQNITAFENGIVGFTKKNGEMVGCV
                     CHQFEPNLKVSVNISGPSAVNVNDTATYYLKISGGPAVLGGCDIATSLGQVITSPLDN
                     TLKREEPFTNAGFELTHISPQSFVNDTLTFIFQYIAPDKPFLTDTIFANGNSVNGDTT
                     SENDIWNYAENFIVNITPSSIISQSSAIPGDFVLSQNYPNPFNPETKINFTLPKTSDV
                     TLYVYNSAGKEISRLIVNERLSQGQYSVSFNSSEYGLTSGVYFYRITASDFSQTRSMV
                     LIK"
BASE COUNT         6896 a         3362 c         3310 g         6772 t
ORIGIN      
        1 aaacaatttt cgtaaaatgg aactgaataa aaaagacgtt aacaaaatat cagttattca
       61 gatatgattc tactttactg agaacttcag gaagcttttc aatatcacgc gcacccgctg
      121 tagctagatg cggctttcct ccaccgccgc ctccaagctc ctttgcaatt acagaaatta
      181 ttttacctgc attaaaacct ttttccttaa ttagattatc actgactgtg cagactatat
      241 ttactttatc atcaatgaca gaagctatta ctgttatgcc gtttgttact ttgtttctga
      301 gataatctcc gatctccctg aactcatccg cgtttcctgc atttattttt tcagctaaca
      361 atttatatcc gttcacttct tttgagtttg aaataatttc atctatttca ccaaaaagta
      421 tttgtaaatt ctgttttagt aattctttat cagcaagctt tttctcttca gaatattttt
      481 ctcttagttc aattaatgac gtaaaagatt catcctgaaa attcaaaagc cttttcatta
      541 aagctgcatc tttgaaatcc gcagattgaa attcattttt aaatttttct aaagaattca
      601 ttactttatc tgaatagctt ataggaagtt catggaaaat tttatctata tctgaaattc
      661 tttcattaag aaaatgcagg atcccgctgc ctgtccgtgc aaagatcctt ctcgtaccgg
      721 ctgcaacgct ttcttcttta gtgatcttaa acaaaccaat gtcagaagta ttttttaaat
      781 gtgtaccgcc gcaaaattct acgctgaagt tatcatcaat atgtacaacc cttactttat
      841 ctccgtattt ttcaccgaat aattttttta cattagggat cttattcgcc tgttcaatcg
      901 aaatatctac atctgtatat atttcaattt cttcattcac tttggaattt actaaatctt
      961 ctatctcatg aatttgttca tcagttactt tatgaaaatg cggaaaatcg aatctgagat
     1021 attcatccga tacaagtgag ccaagttgtt ttatatgttc accgagaatt ctcctgagag
     1081 cttcatgaag tatatgcgta gcggaatgat ttctctcaat ggatcttctt ctttccagat
     1141 ctaatataat ctttactcta ccatctttga aattattgag atcactgttt tctgatataa
     1201 gaaaattctt ttttgaatct ataatattta ttttaaaacc ttcatcagtt tcaataaatc
     1261 ctgtatcact gatctgtcca cctgattcag aaaagaatgg attatttttt aatacaatta
     1321 aattcttttc ccgatctgga attaccattt cagtaaaaaa atcttttcct gaaaatttgt
     1381 aaggatcata ttctgtatca aatttatttg cagtatcatc taacaattca ggagcttcat
     1441 atactatctc ttttcttgaa gactttcctc tttctttttg cttttccatt tcttcttcaa
     1501 atactagaag atctattttc agtcccagct cttttgccat gacttgcgtt agatctaatg
     1561 ggaatccgta tgtatcgtaa agtttgaatg cctgatcacc cggaaaaatc tttgtatttt
     1621 tttctttgag tgagttagca atttcctgaa ataattttat ccctctttca agtgtcagat
     1681 ggaaactttc ttcttcggct tttataactt cttttacaaa atcctttttg gaaactatct
     1741 cagggaatac ctctccaaaa ttttctatca aagtatctac aagattaaat agtatcggct
     1801 catgataatc aagtttactt gcatgattcg aagcacgcct cagaatcctt ctcagcacat
     1861 agccacgtcc ttcattggaa gggatcgcac cgtcactgat agcaaatgtc aaagctctta
     1921 tgtgatctgc aattgcgtta atggaaggaa tgctgctgcc gctatattcc ttttttgtga
     1981 tctcacagat cttatttata attggaagaa aaacatctgt ttcataattg gattttttat
     2041 tttgcaaaac tctgactatt ctttcaaaac ccattcccgt atcgacatgt tttttgggaa
     2101 ggagttcgag ctctctgtct ttttttctat tgtactgaat aaaaacaaga ttccatattt
     2161 ctactacttc ttcaagacct gcatttacat cttcaggctt acatccatta tcagtactgt
     2221 cataatgtat ctcagagcaa ggtccgcacg gacctgtatc gcccatttcc cagaagttat
     2281 ctttctcacc aaatctgaga atatgcgaag gatcgacatc agtttcattt ttccatatat
     2341 caaaagcttc ttcatctgtt tcaaaaactg aaatccataa acgcttctta tcaagtttcc
     2401 agacatcagt cagtaattcc caagcccaga agattgcttc ttttttataa tagtctccga
     2461 atgaccagtt cccaagcatt tcaaaaaaag tatgatgata gccgtctgct cctacttctt
     2521 caagatcatt atgttttccg cctgcccgga tacatttttg agtatcggca gcacgggtgt
     2581 aatctctttt gcctgagccg aggaatacat ccttgaattg attcatccct gcatttgtga
     2641 atagtaatgt aggatcattc tgcggtacga caggggagga cggcacaata gtatgctcct
     2701 ttcccttgaa aaaatcgaga aatgattgtc ttacctcttt agatttcaat taagtataaa
     2761 aaaaaatttg taagtgttta aaataagttt aatgaatgat atattaaatg gtattaacct
     2821 gactttttaa gaaaattttg agaagaagca gaacaggata attgttaaat tggttttcat
     2881 gaatgtacag gtagtttcaa aataaaatta aatattatca gattctactg ttcttttttg
     2941 agcgatgaaa aaagaaccaa aaaaatcgtc ccgggtaccc gggatgatat atagccggaa
     3001 attctttcgc atgcctaaaa aaatataaac tcctcaaccg ttagaaaaac actaacggat
     3061 tcgtcataca ttatattttt gtttaacgtc atcgctcaga atttcttttc ggtaatttat
     3121 cacaggcgga tattctatct aagtatttca ttaaattaaa ctgtgggtta tattttcatt
     3181 ttaaataata aactgaattt gaaaatattt tttaaaatat ttgttccatt ttcaaaatac
     3241 tttggacaat taagctgagt tttataaatt tggatgaatt atttgggagc agttttgatt
     3301 gcaagcagat gccaggttac accaattcct attaaagcca aaagaattct gacataaatt
     3361 aattcagtca gccatattgc tgaaattatc attccgatcc agagcattgc aattgacatt
     3421 atttttgagt taagtgtcat cccgtttcct gaagtatagt tgctaagata ttttccgaaa
     3481 aatctgttat tgtgaagcca gccatgaaac ttctctgagc ttctagcaaa acaccatgct
     3541 gcaagtataa aaaatattgt cgccggtaac agaggtaaaa aaattccgag tattccaatt
     3601 gcagtaagta ataatcccga gatcagcagt aaaatacgta aagccggatt cgaacttatc
     3661 tttagttctg cagatctgtt ttcgcttttg tccattaaaa aattatttaa aaaaattctg
     3721 ataaaattgg taatttttaa gtttatatta aagtgaaatt tatcagtaaa ttaaagcacc
     3781 aaattcaatt tttataacag gataagttga aagtaagccg gatagattaa tctgaaatat
     3841 ttaagctgca aattctttaa ttcttaataa ggaataaaat gtatacaaaa ttaaaaaatg
     3901 cattggtatt tgcaaattca aatgcttatt tttataaaat tttttactta attaatgatc
     3961 tttatataat gaaaaaatac ttcctaagta tttttgcagt attatttatt gcaaatcttt
     4021 cctactctca gttaccaaat caaaacactt atcttttcgc tcaggtagat caatatcaaa
     4081 gttattcagc tttatgggga tacaaagctc cggacagcag ggaatatgct attcttggct
     4141 gtaatacagg aacatcattt atagatatta ctgattccgg aaatgtacgt caagtagatt
     4201 ttgtaaccgg aataaccaac aactggaggg aaatgaaaac atattcccat tatgcatacg
     4261 ttgtatcaga agcaacaaac agcggacttc agatcatcga tcttcagtat ctgcctgatt
     4321 cagtagtttt ggtgaaaacc tggtcttatg caggatatac taaaacacat tctattcagc
     4381 agtccggaag ttatctttat ttaaacggag gtaactctgc tgctaacggc ggtgtaactg
     4441 ttgtggatat aagtgaccct gtaaacccag tgaaaatggg acaatggaca acagaatatg
     4501 ttcatgactg cagaatttta aatgatacaa tctgggcgtc aaacatttat actggagaga
     4561 cttcaattat aaatgcaaca aataaaaatt ctcttgattt tgtcaggaca tggagatctt
     4621 atcctacttc tgaagtttca actcacaaca gtgcatttac tactgacaga aaatattgct
     4681 atactacaaa tgaaatatca tcaccaaacg gcagattaaa tatctggaat atcgaagatg
     4741 tcaacaatat aacttttata agagaatggc tgcctacagg catttctact tcaatagttc
     4801 acaatgtcga aatttacggt aaccttgctg tcattgctca ttacagagca ggtatcagaa
     4861 ttcttgatat tacaaacgga tcaaatccga ctgaggtagc ctggtatgat acatatccac
     4921 aaagtaacag tgcaagctat agcggatgct ggggtgttta tatgttccca tcaggaaaga
     4981 tcatcggatc tgatatgagc ggaggactga ttgtcataaa gactttgaat gtattgactg
     5041 gagtttcaaa taattatgta aattctattc ctgaaaaatt cagtttagat cagaatttcc
     5101 caaatccttt taatccatct actactatta aatttaattt aaaagataat tctaaaataa
     5161 agctgagtct gtatgacata gcaggtataa aaattgctga tctgattaat gataacagag
     5221 atgccggatc ttatgaattt aattttgatg ctgcaagata taatctttcc agcggaactt
     5281 atttttacag tttagaagga aatgatatta atggaaattt caccgatacc aaaaaaatga
     5341 ttttaattaa ataatttatt gccaaaaaaa ttaatgagaa aattaacagt actttttatt
     5401 ttggttgtga tttttttagt aagcagtaat gctaattcac aacttccaaa tcagtctaca
     5461 tatttattaa aaaacttaaa tcagcattca gttactcaat tccaatattc agcagtttgg
     5521 ggatatgtag cccctgatgg cagagaatat ggaatactcg gatgtccggg tggaactgca
     5581 tttatagatg ttactgattc tgcaaatatc cgcgaagttg attttgttcc aagtcttact
     5641 aacaacaact ggagagaaat gaaaacttac tctcattatg cttatgtagt ttccgaagca
     5701 acgaacagta aacttcagat tatagatctt caatatcttc ctgattctgt aagtcttgtt
     5761 aacacatggt cttatcctac ttatacaaag actcacagca tttcacagtt cgatcattat
     5821 ctatatctta acggcggaaa ttctgcatca tcaggaggaa ttactattct ggatgtaatc
     5881 gatccggtaa atcctgttaa gcttggtcaa tggactgatc tatatgttca tgactgcaga
     5941 gttgtaaacg atactatcta tgctgcaaat attaatgatg aacgagtttc aattattaat
     6001 gcaactaata agagtgcacc atctactgtt agaacatttg taaatcttcc gggttcaggt
     6061 cctcataata ctgcgctttc aaaagacaga aaaagattat ttgtaacaga tgaaatcggt
     6121 acagctccct acagacttaa agtatggaat atcgatgatg tgaataatat tacttatata
     6181 acaagttgga atcctactaa tattactaat tcgatagttc ataatattga aacatataac
     6241 gatattgcta tagtggcaca ttattcagct ggaatcagga tactgaatat ttctaatccc
     6301 gatgcaccgc aggaaattgc atggtatgat acttatcctt taaacaataa tgaatcatat
     6361 aacggatgct ggggagtgta catgttcccg tcaggaaaga tagcggcttc tgacagacag
     6421 accggatttt attgtatcaa atcaactgaa gtgctcacag gaatttcaag taatatatca
     6481 gcaagtgtac ctgagaattt tagtttaaat cagaattatc ctaatccttt taatccatct
     6541 acaaaaataa atttttcttt gccaaagtat tcacaggcta gtttgaaaat ttataatgta
     6601 aacggaaaag aagtagctga attgtttaat gatacaagag atgcaggtaa ttatgaagtt
     6661 tcatttgatg ctgcaaaata tggtcttgca agcggaactt atttttataa tctgaagaca
     6721 caggatttta atgaaaccaa gaaaatgaca atacttaaat aatattaaat tttaaatatt
     6781 aaattttaaa taatttttaa ctacaaagtt tatatcccgg atcgaaaagt ccgggatttt
     6841 tttttgtgaa atttaaatat aattaaagag taaaaatatt aaccgatttt gagtaataaa
     6901 tttatatgta gtttgtaatt ttaaaataaa ctaagtttaa gaaaataaat tctaaggaaa
     6961 tatgaaaact ttaatattac ttttcacaat aatattcata cccgaaatat cattccctca
     7021 aacatttttt gaaaaagaaa ctaccggcgc aattgtccaa aattttaatt ttaatcatat
     7081 aatggcatgg ggtgattatg ataatgacaa cgatcaggac gttatgatca atggcttgaa
     7141 tgatcagtgt tctacatgtt acatgccgat aattttttta aggaatgaag gaaacggaaa
     7201 ttttatcagg atctatgata atcctattgc tcagactttt ataacaggaa gcggaatggc
     7261 atggggagat tacgataatg atggatggct agatcttttt atctgcgcta cactaaatgc
     7321 aaggaataga ttatttcaca atgacggaaa tggaaatttt actgagatcc tgaccggtgt
     7381 aatagtaacc gaaatgaatt caagccagag ctgtaactgg gtggactata acaaagatgg
     7441 atggctagat ctttatgtaa caaaccgtta cggaatatac agtaacagac tatataaaaa
     7501 taacggtgac ggtacgttta caaaaatact ttcaggcagt attgtaaatg atatcggcga
     7561 gagcagggga agtacatggg gagattatga taatgacgga tggccggatc tgtttgtagt
     7621 gaattttgga ggacaaaatg attttcttta tcacaataac gggaacggga cattcacgag
     7681 aatattcaat ctgccaatgg taaattacgc tgcatgggga tcatcctgtc actgggtgga
     7741 ctttgataat aatggatgga ttgatttgtt tgttacaaat aataattctg caaacagatt
     7801 atattacaat cacggagatg gtaattttac tttgagtaat tcacttctca gtcagaaagt
     7861 aaatggactg ggatttacct ggggcgacta tgataatgat ggtttaactg atctgtttct
     7921 ttgtgactgg tacggcacaa acactttata caaaaatcag ggcggatcaa tgtttgtaag
     7981 agtttcaaat gaaataccat cacaggaagg gctacacagc tctactgctg cttttacaga
     8041 tatagatctt gacggtaaac ttgatttatt agttgccaac agactcgata acccatttaa
     8101 ttatcaatat aacaatattg gaaatacagg aaattatcta acaatacagc ttaaagggca
     8161 tgcaagtaac aaaaatggag tcggtgcaaa aattaaaatt gtaatcggaa atacacctca
     8221 gtataaagaa ttattattgg gaagtaatgg tcataactct ggaagcacac tctggccgca
     8281 ttttggaatt ggtaataatc agggagtaga ttcaataatt gtgaactggc cgtctggtgc
     8341 agttacaaga tcaggatatg tcaatggaaa caggattgtt attattgatg aagattttgg
     8401 agtgatcgga ataaatgagg agaacataac agcagtacag tataatctgt atcagaattt
     8461 tccgaatcct tttaatccat ctactaaaat taaatttgaa atcgcgaaaa attcatttac
     8521 tgaattaagt atttatgatg taaatggtag aaagattgag acacttgtaa atgaatttaa
     8581 aagacccgga tcttatgaga tagaatttaa cagcaacaat tctgttagaa atctatccag
     8641 tggtatttat ttttataaac tgtcagcagg ggattatgta tccactcaga aaatgattct
     8701 cataaaataa tttttattat aaatacaaat atcagatata ttttaaatgc tgaccgattg
     8761 ttgaaattat cccggatcga aaggtttggg atttttttgt aagaacaggg ttctttaaat
     8821 caaaaactgt gagttgaatt gattcaatgt ttaacttatt ttgtgaaaaa ttataatcct
     8881 gtataaatat aaattacaat ttttcaaaaa ataaattaac aaaatggaaa gtattacaca
     8941 aaagcgtccg ctcacacgca gggaatttaa aatgaaatat tataaattgg caatgattgg
     9001 tactatgatt tttatttttt caattacagt gggtatgctt ggatttcatt ttattgtaaa
     9061 acttaactgg gtagattcgt ttttaaactc atcaatgata ctgggaggta tgggacagat
     9121 atcagaatta aaaactgatg cagaaaaaat atttgcaggg tgttatgctc ttttcagcgg
     9181 agtgacattt cttaccagca ttgctatttt tattactcca cttctacaca gagtattaag
     9241 cagacatcat attgatctgg attcaaaaga ataaatttga taaaaaggat ttgatgattt
     9301 aatatctttt ttaaaaatga aatttacttt tgaaatactt cattagcaag accgtcggga
     9361 tctgatatta ctgtccattt atcttcatga ttaataggtt ttaatatctt tattttataa
     9421 gtctcaaaat attcaactgt tgctttaata tcatctgcat aaagatcaaa agaaaattca
     9481 ggaattgtac tatgtgaaaa ttttctgtca ttctttttcc caaatggaat gagcagggat
     9541 attttatcag tacacttcat tcttacagct ccttattcat gttcgattac ttcaaatctc
     9601 agaaaatcct tgtaaaatcc cggatttttc aatatccgaa cttaaatatt tatttcacca
     9661 atttcaaact tcatttattt gtataaaaat aaattacaat aaataaataa ccgggaaaat
     9721 taatctcccg gtttaaaaaa tactattcga attatttact tattatatca gaatcaaatt
     9781 tttctttttg atcagaagtt attaattaat agattctgat taaagtatct tagtatctgt
     9841 aaccaattcc aagtgtcaaa ccatattcat taactgtcca gttgcgtgca gttttatcat
     9901 aattgacacc tacatattta aatcctaaag ataatggaac attaggtgat aacaaatatg
     9961 caaatccggc attcatttgc catgtcattt gtgagtttac accaaatccg ccgatatctc
    10021 ctttagtcca tgcagcccac ttcatatctt tgcttaaaag atattcagca tttataccaa
    10081 tgaccggatc aaaccaggag ttggtatatt gacccatttt tgcaactgta gatgtgttat
    10141 caattattgt tgattcaaca tccagattcc acaaacgtcc acctgcgtag acatccaccc
    10201 ttacagttct gctggtggat ggaaataaat atgctaaaaa cagatcataa attgcttgtt
    10261 ttacagtcca gttagaactt aatatttgtg gtgtactgac aggaatttct gtaccaaatc
    10321 ctttaagatt taaatagcta atatcatata caaaacttac tctttcgtat ttaatctttc
    10381 ctgtaacagc ggctgccatt ttcagatatt tcacagcatc agagaaactt ttattgaaac
    10441 catatttttc tccatttgga ccatatccta ctgttcctcc tatagaagta aaccatacat
    10501 agggaatgat actgaaactg aatttcttag gttttgaagc actttgattt gttagatctt
    10561 ttttaagaga taatgaattc aaagggattt cagaattgaa tttctctaaa ttcttatcca
    10621 gaggtgaatc tgttttttca aatttaagac tggaaagact ttttacgtct tgcaactttg
    10681 gataatttcc ctgagcattt atattgaaac taaatgcaaa cagaagaaaa aaaagtgtaa
    10741 tctttttcat tttaattttt atttattaat tattaaaact taatttacat actcttttta
    10801 atatgactcc ccaatacata ccaatgagtt tgtaaataag tttagattag ggggataaaa
    10861 ttttagttca ttattaaatc agcaaaacct taagtaattt aaaattgaaa attaaattac
    10921 ttcgggtttt ttataattag aatttttaga aatttttaaa ttaattcgca aaacatttat
    10981 tattgttaca ttaattatag tggttctaaa ttatttgtag gggaagttct gttaaaaaat
    11041 attaactttt caaaataaat gatcagcaaa gaaatgtttc aaacagcaat acaacaccca
    11101 taaacactgg tcattcccgc taatacagga atctcaaact gaaaccagac tttatttctt
    11161 aaattaacca aatcattgca agttataaag caccattata gtttacttta tttattctga
    11221 ataagtaaag aaaatattcc tgaaaaatta tattttaaac ctcacaatct aacactataa
    11281 ataaataaat gcactgaatt taattttgaa aatgcagtaa tttatttttt atataaattg
    11341 aatatcaatt taaaattcat tcacttttta aaaatcttaa cttatcaact ttttaatcct
    11401 ttccgatttt aaaaaatcta aatcggatca ttacttttca gtatttattt atctaataag
    11461 atgatatttt aaattcaatt ctttatattt gtaaaaattt tgcaagatga aaagtagaat
    11521 ttatatttaa aatcttttgg ggggtaattc cattattaga aataaattaa ttatcctcct
    11581 gtcgataaaa ctataaaata taacatgaaa gaatttttta aaaaaaataa attaaagata
    11641 cttgtattcg cagtcagcat ccttttcgtt gcatatattg tcaacggtat taatgaacat
    11701 actgtgattc cggatcacat acattccatt gatgataaag cttccgaaga atggacagat
    11761 gaggaaaaaa taacagtgct aaaacgaact ctacagaatg aaccggataa tatagaagcc
    11821 atgcttaagc tggctgatct ttatattcat acaggtgata ataaatctgc aaaaaaaact
    11881 atagataaag tattggaaat tgatccctac aataaagaag ccactgtact tcaaagtgaa
    11941 ttaaaataga aattaatatt ttatttccaa tatattgtaa gagaatttat caatgatgtt
    12001 gatgagagaa acgataatta ctaaatttcc agtaatcgtt aacgacctaa aataattgaa
    12061 aaatggtaaa gaaattttta ctcaattttt tagaaattaa gtataaattt attatagcac
    12121 gattcagaat aaattacaag taattcccct caaaaaaggg tgagcaaagg gggttgttgc
    12181 gggaagaata ggatgcaaca aattcttata aactgaacat gtaatcagtg ggttctattt
    12241 aaactcaaat ttacaaaccc ttcccagcct cacctttgga cgaaggggag gagcaaaaac
    12301 agttcataat gcttttggtt ctggctatac cagtgcaaaa attataaaat aaaattaaaa
    12361 atcttggaag taccatttac atattttatc acaaagaata acaaattaat aatttcaaga
    12421 aataacaaac agatcaaaac agtcaaagga aagggatttg aagatttcaa agtattactc
    12481 aagggcgggg atgaagataa aatacaatta aaacttgcaa aacttaccgg ttattataag
    12541 catggcaatg agaaataatt aatagaatta agtttcagcc ttttctttta aatcttttcg
    12601 atttaatttt aatccaaaca aataagtcca tccaataaaa agtaactgca tcggaattct
    12661 gaaatataaa tattcaatac cgggtccgga attgtctccg gtccgaagat caatgttatt
    12721 tattgcagca ttaatgtttg caggcaaaat tgcgataaag aagattatta acagtaaaga
    12781 agtgatcctg ctgtaacgat caattatcaa tccgatagct gccaggaatt caagaatacc
    12841 agtaagatat accagaaaaa ttcggaatgg aatgaaatcc ggtatcatct ttgccatacc
    12901 ttccggaaaa ataaaatgtc cggaagcagt taacaaaagc attgcagaca ttgctatcct
    12961 tcctgaaagc gaatgatcaa attctccttt ccttactctt attataattg aactgattat
    13021 ataaattcca aataaaagat atagtaattc catttcaaaa gtttatttta agaaatatga
    13081 aaagtgtttt gatgattgag caactcaata taaatttatt taatgagaat gaaaataaat
    13141 tttaaattat aaacaaagtt caatttttaa atttcagatt gatttgaaat aaagtaattt
    13201 aattccaaat tataataaaa ttcaattgat gtcaaattta ctgttacagc atataagcaa
    13261 ttctctttcc atcagtttga aacaggtcac tgctactgtc gatcttctta atgaaaatgc
    13321 aaccattccg tttatatcaa gatacagaaa agaagctacg ggaagtctgg atgaagtcca
    13381 ggtgtcggat atttcatcgg aattaaaacg gctaactgaa ttaataaaac gccgtgagta
    13441 tatcctgaaa agtatttccg atcaggaaaa gctgactccc gggctggaag caaaaataat
    13501 aggatgctgg gatccgatcg atcttgaaga tctatatctt ccttttaaac ccaaaagaaa
    13561 aacgagagct gctgctgcct gtgaaaaagg tcttgaacct ctggcaaagc ttatctggtc
    13621 acaagaaaat tctgatattt tcaatgaagc aaaagcattt gtaaatgatg atgtagtaac
    13681 aatagaagat gcactggaag gtgcaagaga tattattgca gaatggataa acgaagatgc
    13741 ggatgcaaga aattctttgc gaaatttatt taaacattcg gctgtgatta cagcaaaagt
    13801 agtaactaag aaaaaagaag aaggtcagaa atatcttgat tattttgaat acagtgaaga
    13861 ctttaggaaa tgtcccggac acagacttct cgctatattt cgcggagaga aggaaggaat
    13921 actcagagta aatgcaggtg ttgatacaga tcttgcggta aaaattctag agaagcaatt
    13981 tataaaacct gataaaatta attctgtaaa ttcttcagca gaacagatag taaaagctat
    14041 aatagattca tttaaaagac tgcttgctcc ttcacttgaa actgaattca gaaatcttta
    14101 caaagagaaa gctgataagg aagctatact tgttttcact gaaaatctga gacagctgct
    14161 acttgctgca ccgctcggtc agaaagttac tatggctgta gatcccggtt tcaggaccgg
    14221 atgtaaactt gtatgtctgg atgaacaggg ttcgcttttg cacagcacta ccatttaccc
    14281 acatcctccg caaaatcaaa tgaaagaatc tgctgaagta ttaattaaac ttataaagaa
    14341 gtataacatc aaagcaattg cagttggcaa tggtactgcc ggcagagaaa ctgaaaattt
    14401 tataaaagga attgtaaaag aaataaaaga tacagaagta tatcttgtta atgaagcagg
    14461 tgcgtctatc tattcagcat ctgaagctgc gagagaagaa tttccggaac ttgatctgac
    14521 tgtgcgtgga gcaatttcta ttggcagaag attaatggat ccgcttgctg agcttgtaaa
    14581 gatcgatgca aagtcaatag gagtagggca gtatcagcat gatgtcgatc agcagaaatt
    14641 aaaagaaagt ttagatcaga cagtaatatc tgcagttaat cttgtaggtg tgaatttaaa
    14701 tacttcgagt cagcatcttc tccagtatgt ttcaggtata ggtccgaagc ttgcaaaggc
    14761 aatcacaaaa tacagaaatg ataagggacc gttcagttcg agagaagagc tgatgaaagt
    14821 aggcggagtc ggaagtaaat cttttgagca ggcggctgga tttttaagaa ttcccggcgc
    14881 aaagaatcca cttgataaca gcgcagtgca tccggaaagt tatactatag ttcagatgat
    14941 ggcaaaagat ctttcgcttc ctgtaaaaga tttaatagtc gcaaatgaaa taattaaaca
    15001 gatcgacatc aaaaaatatg taaatgataa agttggaatt ccgacactcg aagatattat
    15061 aaatgaatta aaaagaccgg gtcttgaccc gagaggggag accactgtat ttaattttgc
    15121 tgatgatata aatgacataa aagatcttaa gatcgaaatg cgtctgcccg gaataattac
    15181 caatattaca aaattcggag cgttcgtaaa tatcggaatc aaggaaaacg gactgctcca
    15241 tatctcacag atcagcagta accgattcat aaaagatccg gctgaagttt taaagctcga
    15301 tcagaaagta atagtgagag ttgtaagtat agatgaagag aggaagagga tacagttgtc
    15361 gatgaaagag aaagagtaga gagaattaag aattaggaat taggaattaa gaattacggg
    15421 ttactagtta aaaattacga gttaggagtt aatgatattg attgttctat atatatattc
    15481 atctcagata aatttaaaat caaaaatcaa aaatctgaaa tatgcaattg ttatagaaag
    15541 aagtaaaact ggtttcggtg cgtatgtttg ggatctgccg ggatgtgcat ctgttgcaaa
    15601 aacaaaaccg gaaataaaag aattaattaa agatggaatc gaatttcatc ttgaaggaat
    15661 gaaattaaat taaatggtta caaaatacct gactccgctg catatattat ggaagttgct
    15721 gtaaaggttg cctgaaaatt tttatctgca aattattgat tcgaataatt attttgaatt
    15781 tctatctgca aatgcatttt tgtaaatcga acttcagatc tactgtctat cccacatgct
    15841 tttaacggga aactatttat cacgataata aaaatttata attccagcat gtccccattt
    15901 gtcattccag cgtgctccca gctggaatcc agtcatatgt ttttctggaa gattattctc
    15961 aggtctgaat tacaccgaca ttaaattttt taatctcgga gttattgttg gcaagttcta
    16021 tacaatatga aactatcttc cgggtatcct ttgggtcaat gacttcatca acccataatc
    16081 tcgatgctgc ataaagtaca tttgtctgtt cgtcatatct gctatggatc tcttcgagga
    16141 aagatttttt ttcttcttca gtaagttctt ttccctgttt cttaagctga cccatcttaa
    16201 tgtccagcaa tacattactt gcctgagatc cacccattac agctatctgc gcatttggat
    16261 aagcaaaaat aaatcttgga tcataagctt ttccgcacat tgcataattt cctgcaccgt
    16321 aactgttgcc tgttataatt gtaatttttg gtacaacaga atttgcaact gcattcacca
    16381 tttttgcacc gtctttaatt attccaccat gctctgatct ggaccctacc ataaaaccgg
    16441 ttacatcctg aagaaataag agaggaattt ttctttggtt acaattcata ataaacctcg
    16501 cagctttatc tgcactgtca gaataaatta caccgccaaa ctgcatctca cctgatttac
    16561 tttttacaat gcttctctga tttgcaacaa ttcctactgc ccatccttcg atccttgcat
    16621 agcctgtaat aatggttttt ccatattcag gtttatattc atcaaaatct ccgtcatcaa
    16681 taattctgcc gataagttca taagtatcat atggttttgc agtatcttca ggaagtactc
    16741 catatatttc cttttcgtta tgatttggat ttttattttt aattctgtca aatccagctt
    16801 tgggcatttc accgaactta cttacaagct cacggatctt ttcgatacac tctttatcat
    16861 ccgccatttt ttcgtcagtg attcctgata tttcagttgt tactgcagct cctccgagag
    16921 attcgttatc gattttttca cctatagctg ctttcaccag atgtgatccg gcaagaaaaa
    16981 tactgccggt gccttcgact attaatgctt catcgctcat aataggaaga taagctccgc
    17041 ctgcaacaca aggtcccatt atagcggcta tctgcggaat tcccattgag ctcatcactg
    17101 cattatttct gaatactctt ccgaaatgtt ccttatccgg aaaaatttca tcctgcattg
    17161 gaagaaaaac acctgcacta tcaacgagat atataattgg taatttattt tcgatcgaaa
    17221 tttcctgagc acgtaaattc tttttgcagg taattggaaa ccaggcaccc gcttttactg
    17281 tagcatcgtt tgcaactatc acacaattcc ttccatgtat tttcccaatt ccaaatattg
    17341 tacctgacgc aggtgctccg ccgtattctt tgtacattcc ataccctgca aaaatgcaga
    17401 gctccataaa gtatgaatct ttgtcgatca gaagatcaac cctttccctg cagtgaagtt
    17461 tatttttgga atgaagtttt tctatagctt tttttcctcc accaaaatga atttcctttt
    17521 taagtgcaat tgtttttttt ataagatctt tataaaattc ttctctcttt tcaaataaag
    17581 tatctttttt taaaccggat tttaatttag gcattggaaa aatttatgtg ggttgaattg
    17641 aacagataat ttataaacag atattatact aaattgtatt aagtaaatat tgttgataat
    17701 tttttgaatt aaatactaag ctatgacctt cctgttaccg ctcagataaa caggttcttt
    17761 tttttctgaa ataacttctt ttgtaataat gcagttagaa acattttctt tagacgggat
    17821 cttatacatt atatcgagca taatattctc aagtatagac cttaaagctc ttgcacctgt
    17881 tttccttttc aatgccagct caactactcc ttcaagggca tcttcagtaa attccagatc
    17941 cactccttcc attctgaaaa gtttctgata ttgttttgtt attgcatttt ttggttcggt
    18001 aaggattgaa agcattgttt tcctgtcgag tgaattcaaa gtagttatga caggcagcct
    18061 tccgataagc tcaggtataa gtccgtaatg gataagatca tccggttcga cctgagaagt
    18121 cagcagatcc tgatcgcttt tgtcttttga gaaaatatct gatccgaaac ctatagaact
    18181 tttagaaatt cttttttcaa taattttatc gatcccgtca aaagcaccgc cgcaaataaa
    18241 cagaatattc tttgtgctta tattaataag tgcttgctca ggatgtttcc ttccgccttt
    18301 aggaggaaca ccggctattg taccttctaa taatttcagt aatgcctgct gtacaccttc
    18361 gcctgagaca tcccgggtaa ttgaagtgct gtcacttttt cgggagatct tatctatctc
    18421 atcaagataa ataattccgc gttcggcttt agagagatcg aagtttgcat tctgaagtaa
    18481 gtggactaaa atagattcaa catcatcgcc tacatatccg gcttcagtca gagtcgtagc
    18541 gtctgcaatg gcaaaaggaa cgtcaagaag tcttgccagt gactgtgcca ggaaagtctt
    18601 tcctgttcct gtcggtccga ttaaaagtat attacttttt tcaatttcaa catcatcaat
    18661 ataattgaaa tcaattgagt caattctttt ataatgatta taaactgcta cggcgagtga
    18721 tttctttgct ttgtcctgac cgataacata ttcatccaag aattttttaa ttgcctgtgg
    18781 agtaagatct gatctgactt tgttgatgga ttttgtagca ctgctgagag tatttttttt
    18841 gatgatctgc attgcatttc ctacgcagat ctcacagatg aaaacttctt ttccgttgtg
    18901 atcagacggt ccttttatca tgttggtaac tttgtcggca ttcctgccgc aaaaagaaca
    18961 tcttgctatc tgatttttag atccggattt tgtactcata ttaaaggatt aaagatattg
    19021 atttaagtaa tatgatataa agtgcgtgat gagggagtcg aacccccacg ggtcgccccg
    19081 ctagatccta agtctagtgc gtctgccagt tccgccaatc acgcagtaaa aaagacagtt
    19141 acaaaaataa ttaataatat tctgaattac aattgattat tgagtttagg taaagtaaaa
    19201 ctttaagtat ttgatatcag gaatttaatt tctcaaatta aactaagaga ttgaataatc
    19261 attcttacaa aagaattctt caatcgagaa taataatcat atataaaaaa agccgaatga
    19321 aatgtccggc tttttagaat ttcgaaataa atttatttta tcagaaccat agatcttgtt
    19381 tgcgaaaaat cagaagcagt gattctgtaa aaatatactc cgcttgtcaa tccatattca
    19441 ctggaattaa aagaaacaga atattgaccc tgacttaacc tttcattaac aattaatctt
    19501 gaaatttctt ttccggcaga attataaaca tataaagtta catcagatgt ttttggaagt
    19561 gtgaaattaa ttttagtttc cggattaaat ggattaggat aattttgaga aagaacaaaa
    19621 tcacccggga tagcactgct ttgtgaaata atggaagaag gagtaatatt tacaataaaa
    19681 ttctcagcat aattccatat atcgttttca gatgtagtgt caccattaac agaattacca
    19741 tttgcaaata ttgtatctgt taaaaatggt ttatcaggag caatatattg aaaaataaaa
    19801 gtaagtgtat cgtttacaaa actctgcgga gaaatatgtg tcagctcaaa tccggcattt
    19861 gtaaatggtt cttctctttt taaagtatta tccagtggtg aagtaattac ctgtccgaga
    19921 cttgttgcaa tgtcgcagcc tcctaataca gcgggacctc cggagatctt taaataataa
    19981 gttgctgtat catttacatt aacagcactt ggtccggaaa tatttacaga cacttttagg
    20041 ttcggctcaa actgatgaca gacacatcct accatctctc cgttcttttt tgtaaatccg
    20101 actattccat tctcgaaagc agttatgttc tgataaatag aaaatataaa tgctgcaact
    20161 attacaaaca ctaaagcctt ataaattttc attgtagaat tattttaatt tgtaaagaaa
    20221 aattaagatt aattaatagt aatttcaatt tagaaataga agttattttg caaaaagaat
    20281 aatttttgaa tttaaattaa aattaacaaa cgagtctgac agaaaatttt ttttctgcaa
//
LOCUS       DAORVQ010000039        20139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1062_length_20139_cov_5.711113, whole genome shotgun sequence.
ACCESSION   DAORVQ010000039 DAORVQ010000000
VERSION     DAORVQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 20139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20139
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_20139_cov_5.711113"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1523)
                     /gene="guaB"
                     /locus_tag="PLX80_05040"
     CDS             complement(57..1523)
                     /gene="guaB"
                     /locus_tag="PLX80_05040"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQY20194.1"
                     /translation="MKSGKITETAITFDDILLKPAHSNVLPRDVDLRTKLTKNIILNI
                     PLLSAAMDTVTESEMAIAVAREGGMGIIHKNMTAEMQAAEVDKVKRSESGMIRNPITL
                     TSDKKIADALLLMQEYSISGIPIINSEHKLIGILTNRDLRFEPDATLPVSEIMTKVNL
                     VTAPIGTDLEKAEVILQKYKIEKLPVVDKKGVLKGLITFKDIQKRKKFPNASKDSFGR
                     LRVGAAVGISDGTIERVEKLIAAGVDVIVVDTAHGHSQGVLDMVKKIKKKFDIELIAG
                     NVATAEATIDLINAGADCIKVGIGAGSICTTRVIAGVGIPQMTAVLECAAAASKRKIP
                     IISDGGVKQTGDVSKAIAGGADTVMLGGYFAGAEEAPGEKVLFEGRSFKVYRGMGSIE
                     AMKKGSKDRYFQDVEDDISKLVPEGIEGIVPYKGNVGDTIYQILGGLRSSMGYCGAKN
                     IHEMQTKSKFVLITNAGLKESHPHDVRITKEAPNYGLK"
     gene            complement(1525..2412)
                     /locus_tag="PLX80_05045"
     CDS             complement(1525..2412)
                     /locus_tag="PLX80_05045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY20195.1"
                     /translation="MIKTFLAVTFAFIFLFSLAFTNNVNADDKKQKKKKSGKKEKIVS
                     KRINPSIISISLKINGLKADENFYGKPDKIASRWQVIINDIRKANLSRQDAIDSLNLF
                     TNQLWDFMDKRIDAGVIKKYSDDEWIFPVRGYGPNAIGGRNGSGYIVGGFDFFDGSTG
                     GHPAHDIFIDDRDQDSRDDNTGEFVEILSMTGGIVIETRKNWTTDMMDIKGGNIVYVY
                     DNFTNGLFYYAHMNDVYVSVGDFVEPGKPLGTLGRTGKNAYPSRSPTHLHIMYVRSYD
                     GDLLPENIYNDLIKVKTIY"
     gene            complement(2434..2859)
                     /locus_tag="PLX80_05050"
     CDS             complement(2434..2859)
                     /locus_tag="PLX80_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20196.1"
                     /translation="MKNYYKPVLALFVLLFLVLGCSKLKELGDSAGGNKLYFCESYSS
                     ATDNCEGKSSKYTQGNLTVMVDLRPSKTKVGVKSVNINVTDLNTGDVVETFKFDTDSD
                     MDYIYFDNVKFDKTGKFKVSALKPDGTVIVTDEIEIVEN"
     gene            complement(3030..3236)
                     /locus_tag="PLX80_05055"
     CDS             complement(3030..3236)
                     /locus_tag="PLX80_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HQY20197.1"
                     /translation="MDDLIKIVSEKAGITADQAKSAIEAIGGFVKDKLPAGIGDKVSD
                     FLAGKDVGGGLADMASGLKDKLGL"
     gene            complement(3277..4821)
                     /locus_tag="PLX80_05060"
     CDS             complement(3277..4821)
                     /locus_tag="PLX80_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQY20198.1"
                     /translation="MDQKEKFIASVNEGYEFKGETITLGGAIFKGENLPGIHVKLPLK
                     TMNRHGLIAGATGTGKTKTLQLITEGLSSKSVSVLLMDIKGDLSGLAQPGEINDKINE
                     RHEKIGMTYTPSSFPVELLSISNEKGARLRATVTEFGPVLFSKILDLNDTQSGVVSVL
                     FKYSDEKKLPILDIKDFKKLLQFISDEGKDEVEKEYGQISTATIGTILRKLIELENET
                     NLFFGERSFEVDDLVKIDSEGRGKVSIIRLADIQNKPKLFSTFLLCLLVEIYNNFQEE
                     GDLDQPKLVIFIDEAHLLFQEASKALHEQINTIIKLIRSKGVGIFFCTQNPTDIPDEV
                     LSQLGMKVQHSLRAFTAKDRKDIKMISENYPISEYYDVDELLTSMGIGEAAVTVLSEK
                     GNPTPLVATLLCAPASRMDILSDAEQEEIVSKSELVKKYNEVIDRESAYEILEKKLND
                     AAAESTVEETKKSSAKEEKSTVEKVLESTAVRQVGRTIAREVTRSFLGVLGIGGSSRK
                     KKSSWF"
     gene            complement(5503..6210)
                     /locus_tag="PLX80_05065"
     CDS             complement(5503..6210)
                     /locus_tag="PLX80_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQY20199.1"
                     /translation="MLKEISGMYESILEFIYPPSCILSDEYISNENSNRYIKDDKFNL
                     LFRISDKDKVDLKNKVFSEYSFSLFAFYEKDDFSKIIYNIKYGGMRNLAIYMGEIIGL
                     EFLKAKDIDEFPKFDMIIPVPLHRSRQRERGFNQSQLMCRGFSNITGIKTADDLLIRI
                     KNTSSQTRLNKSDRRLNIENAFVLNEKYSDTLKGMKIILLDDVITTGATINEAVNILK
                     QHGAGDVLCCSLAMARD"
     gene            6823..7881
                     /locus_tag="PLX80_05070"
     CDS             6823..7881
                     /locus_tag="PLX80_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20200.1"
                     /translation="MKKIKSVPVKYLLVFIFSVTFFYSCSDDPVSNDPVDPTYQYDSA
                     RFSWKVDTLYGPGFFAGLWAKDTNDIFITNTFSSSLYRIQNGVKSYISYPVNTYLGVV
                     TGDEMNNGFVIGGILIDTLVQPFFQKFDGNSFIEIPNPQNLKQSLTFNAVFVKNENEM
                     WIGANGYILKFDGVNLYSFQLEDSRTKALKFFYDEFNNFKLLASIHHLDTDRTEFIIY
                     EYTGSQWINIFSDIDSPRPLRYNIFNKYVSAANLNTIFELTGNTLTPKVTITPPGSGF
                     TMDGYSFNNILVPARKYTDFCRTSLFHWNGIKWSLELCNYWVDPTTDVYMINENYYYV
                     VSYNQNGTTFIMQGKKNN"
     gene            7903..8214
                     /locus_tag="PLX80_05075"
     CDS             7903..8214
                     /locus_tag="PLX80_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20201.1"
                     /translation="MKTNRNVQFFITLIAIFYISNIALSQEWVTNNYGNSGHSLDFLN
                     ANTGFIGLTVYDNGYKYKILKTTNKGVSFTPIWSTSSNQYNQQSFAFDMINEYVGYVY
                     I"
     gene            8493..11360
                     /locus_tag="PLX80_05080"
     CDS             8493..11360
                     /locus_tag="PLX80_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20202.1"
                     /translation="MVGYYHNFQELGQIRRFTLVSDNAGADFGPEYDGGIYGTQFSNV
                     QYLPESFTDYRIIGIENYGALDTLNGTFCYDNFSATNKYKITNDHFNIGGLSFINATT
                     GYSYVNDKLYKSTNSGVNWNVIYAFAQGSLKSNRNALTAFNEIIYTVDVIGNLVTHTL
                     STNLSTFYDNISTPGSFSFDNVSYNTPSQQYLRGGNSKLNSSLIINPNQSDERIFYKW
                     SDGNMTGNYDTDSNHYYFDMSGTTIANYYKTKQLSTSSEAISNIGQTKVLKDNWGAIN
                     QIHQSMNGGIFYTKSYNNGNNYKQEEIINSSIPHAIADGNKNPDLYETKWYGTIAGGY
                     PVNKNMTACWERYNPSTEKTEIKTAVRDYYIASDTVFMWDAWEDANYSDVFTSFNSSP
                     SYNSKPKIFALAMDPGNNLGNPLTYFFIIPHLRPYSGGNKLQVTCRKGTDYYAEFSLD
                     SGDISDFSAIDSIKCSLGYLEINFTYRKGNQILYRNIAFEYQLNLPIRKLDLEGPIDV
                     SSGDGYSSRFKPDISLMNNVPVVSYAASFNANVYVEYEGGGSDLVPVSRYPIVKVQRI
                     NNTSWSNYVIYNSNSVQQTPDIEGSNSTLSYIINYSLGNGQFKKVVNVSDYPGYLCQP
                     NTFSGTDSRLVKNSYSGHFGNNLSLLTLSPQNSLQKVDKQNFIITNITSSDSYDVDNI
                     DGVVNLDTIKYSFKLGPILIKEPNGEVEGGTFGIEQAENEQILTGVEFNENLKSYPFL
                     LNETQALLLGCNATYIKDNAYSSISSVPYTVNLYNKSTGLLHRTLFQDTIHGEDSIET
                     EFLRGFFITNIPNGEDSFYVKLEPDNSFDDGNFYINPVYDVEDNGDNPNSSNVAVIFE
                     DNKFESHSENIPQDFSLHQNFPNPFNPSTVIKYDLPKEGFVKIKIYDLTGREIRSLVN
                     EVKQAGSYSTVFNGANLSSGVYFYKLETENFRQIKRMVLLK"
     gene            complement(11555..12829)
                     /locus_tag="PLX80_05085"
     CDS             complement(11555..12829)
                     /locus_tag="PLX80_05085"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20203.1"
                     /translation="MKIGILIFLFFIDSKVCIPQSGWTNLNSGTDSSLNSVQFINSLT
                     GWCIGNGGIILNTTNGGFTWEKQNSGFNFNLNSMCFTSLTTGYIAGDSGLIIKTTNGG
                     KSWNRTSLISYHNLTSLNFINDSTGFAGGSNLDNDNPTNKIYKSIDFGYSWDSIEVQT
                     EFTITRVHFVSSQIGWAVRLPPALGGAALIKTIDSGSNWSFSFFTNDNIYSLFFLDSL
                     NGFISSSIPFGIKGILRTTNGGDNWIPEIFPVSGYRMNSIFFINNNYGWAAGEYKIIE
                     ATTNGGISWNTQTNPKLGTTFNSIYFTDSLTGWVVGDSGIILKTTTGGILTNFSIIST
                     EIPDKFSLSQNYPNPFNPSTVINFSIPKSGLVKLKVYDITGREVEVLVNEIKAAGNYR
                     YDYNSINMNSGIYFYKLESDGFTDIKKMAFIK"
     gene            complement(12857..14209)
                     /locus_tag="PLX80_05090"
     CDS             complement(12857..14209)
                     /locus_tag="PLX80_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20204.1"
                     /translation="MADKVSKIDKNGVVTFYSTYNAARTASGNNPGDHDLIQILADLT
                     EQIILKDKVDIWIMPGVVIDQDPSNPGETITDDNETVECTIYGYGVIKNTGSSSCIRV
                     ENPGSKLSVECDYIQNVNGVAVNISPSLKFHLKCNYVYSQNLQAIRIGKFAEPGMVEE
                     VRLDVTKVETGDVNNSSTGTTALSTRGNGFVRIDEIICNNLGHCLSHQEGTITARIKK
                     MTSIMNRSGGNVSTVHLAANQGSAGQKLILYFDEINCFNGATNNALCGIECTIGTGIF
                     IGRRVYSEIISSLDGAAIQIGGANTKGYLKCDNVTSLNHVALVLNDTNEQITIDVNSI
                     EFGGAAGIFSNGSVSNRGNFFIRNAKIKSMSQSTSAKGIFINNIYPIFTLNNLKIISG
                     SDVNSNIFIGNNTIDVFNLGWFGNSGIDQTKIIQKVGVGIDPMDLGYNYQTIIDPLLD
                     "
     gene            complement(14254..15783)
                     /locus_tag="PLX80_05095"
     CDS             complement(14254..15783)
                     /locus_tag="PLX80_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY20205.1"
                     /translation="MNSQVSMEWSKRFNSGTGNSIAIDNTGSIYIGGTEGSGSNTNFI
                     IIKYDPSGMQKWTAIYNGPGNSYDGIDEIAIDGSCNIYATGSSTGINTGSDFTTVKFS
                     NSGYLQWVRRYSSNGNGRDASYSLSIDNIGNVYVTGESKNNIGGIDCLTLKYDSVGNQ
                     LWINNFNRGLNSSNQGFDVTVDDSFNVYLTGRSDGDFMTIKYNKLGFQQWAVFYNGPM
                     NGNDAARSIEVDNLGNVYVSGSSTGDMFYYDYATVKYNSDGEEQWVRRYNGSSNFQDQ
                     LRAMIVDSIGNVYVTGNSTESGTGYDYTTIKYNTNGDILWITRYHNGLNDIAFAMDVD
                     DTGNVYVTGESDGNGTSDDYATVKYDSSGQQKWVKRYDYSAQFSDFATALAVDNNGSV
                     YVTGSSNRDILTIKYSLLTGVNPISFVTPSEYNLSQNYPNPFNPKTFISYELRVSSFT
                     KLIVSDVLGNEVTTLENENKPAGNYQIEFDGANYPSGVYLYRLEIDGNIIDTKRMVLL
                     K"
     gene            complement(15847..17157)
                     /locus_tag="PLX80_05100"
     CDS             complement(15847..17157)
                     /locus_tag="PLX80_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20206.1"
                     /translation="MATEVAVITAATGAQFFYSTYAAARTAATAYDKIQIWADLTNEQ
                     LLLKDKVDIWIAPGRIIQMTDSSPIILDNDGGYTSAVEVNISGNGFFKNTNEKYGCVK
                     IINKDSKVSITCDNLENEGSDPTSLDGTTVYIESCSKFYLNCDKIVNSKQRAIYFEDE
                     VPYVNIRANIIESGDYSGGDAVSFRGIGFLTANEIICKNDASCLFFRGGSLTANVLKL
                     TTQSSGNASAGTVRMSGDSGGQDLTLYFDEIQNLNINGGDAVSANYGNINLIGRRIFS
                     TDGLSLDLASDANILVDEIISVTKGINIHNTSTQKIIIDSNKIEGSNGNDGVIRSSTG
                     SNYVIRNAKIINTYTGSSAPFSRGIFISDNNQANDQTIEIENLIIVTGTGSNDASIYR
                     DGNQNIDIKNLTLFVKKDISSNITLKIGTGITGNFKYIVSMDVS"
     gene            17595..17789
                     /locus_tag="PLX80_05105"
                     /pseudo
     CDS             17595..17789
                     /locus_tag="PLX80_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
     gene            complement(17984..19585)
                     /locus_tag="PLX80_05110"
     CDS             complement(17984..19585)
                     /locus_tag="PLX80_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20207.1"
                     /translation="MNVKILLVIILFVFIFNKGADPQITNFRIHPSGNAQIEPAIVRS
                     PVDPNVMFAVAYTISNSFRSEGNYFTTDGGETWFGSDTCNSGSSTNNHGGDPGPIIDK
                     DGRFILTHQGGFIVGMFSNLSTNFGASWTSNVLIASGDQDKGTPGTDDISTSPFYGRS
                     YLVWTKFLNPFPIVSSYTTNGGLNWSSAVQINASPQGYVSLGADIKVNVSGEVCVCWA
                     GVLTSSPQNEKFCGFARSSDGGNTWNVNENIYAMNGIKAASFQPWNIRVNGYPHIDLD
                     KTGGQRNGCIYIVTGEKNLSPAGSDADIVFHRSTDGGGTWSSGVRVNQDPINNGKVQY
                     FPAINVDDFGGINVIYYDNRNISSDSMDVYLSRSTDGGTSWTDTRINDHRFVPKTVTG
                     AVGSGNQGDNIGITSIGKKLWPVWMADFTGKYQIWTAPINISEIGINQISSLIPDGFS
                     LLQNYPNPFNPSTVINFSIPKSGLVKLKVYDITGREVEVLVNEIKAAGNYRYDYNSIN
                     MNSGIYFYKLESDGFTDIKKMAFIK"
     gene            complement(19639..>20139)
                     /locus_tag="PLX80_05115"
     CDS             complement(19639..>20139)
                     /locus_tag="PLX80_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20208.1"
                     /translation="GFKFEMLRGIELVVNYSYSDFKYDDYFAVNYSAGGDTLVSDYSN
                     NVVPAIPNSIFNFILNYQFNISDKYSGMLLWDMDYISDMFADDANTVVVPSYFYGNIM
                     AGVTMNISDFYCILTSGIKNIFDEKYVGFVNVNANPELNPSERRYYETGEPRNYYLGF
                     NFGYKF"
BASE COUNT         6506 a         3655 c         3032 g         6946 t
ORIGIN      
        1 gttaatagtt aatagttaat agttaatagt taatagttaa tagttaatag ttaaaattat
       61 tttagtccgt agtttggagc ttctttagta attcgtacat catgagggtg tgactctttt
      121 aagcctgcat ttgtgatcaa tacaaattta gatttggtct gcatttcatg gatatttttt
      181 gcaccgcaat atcccattga agatctgagt ccgcctaaga tctgatatat tgtatcgccg
      241 acattacctt tataaggtac gatcccttct attccttccg gtacaagttt actaatatca
      301 tcttctacat cctgaaaata tctgtccttg cttccttttt tcatagcttc tattgagccc
      361 attcctctgt aaactttaaa acttcttcct tcgaataaaa ctttttctcc gggagcttct
      421 tcagcaccgg caaaatatcc gccgagcatt accgtatctg ctccgcctgc aattgctttt
      481 gaaacgtcac cggtctgctt cactcctccg tcagaaatga tcggaatttt tctttttgat
      541 gcggctgctg cacattctaa aactgcagtc atctgcggaa ttccaacacc tgcaatgact
      601 cttgtagtgc agatagatcc tgcgccgata ccaaccttta tacagtcagc acctgcattg
      661 ataagatcta ttgttgcttc ggctgtagct acatttccgg caataagctc tatgtcaaat
      721 ttcttcttga tcttttttac catgtcaaga actccctgtg aatgtccgtg agcagtatcc
      781 acaacaatta catccacacc tgcagcgata agtttttcaa ctctttctat agtcccatcg
      841 gaaattccaa cagcagctcc gactctaagt ctgccaaaac tgtctttact tgcattagga
      901 aattttttcc ttttttgaat atccttaaat gtgataagtc ctttcagaac acctttctta
      961 tcaactacag gaagtttttc aattttatac ttctgcagaa tgacttcggc tttttcgaga
     1021 tccgtaccga tcggtgcagt gaccagatta actttagtca tgatctcgga taccggaaga
     1081 gttgcatcag gttcgaatcg cagatccctg tttgttaata ttccaatcag tttatgttcg
     1141 gaattaataa ttggtattcc tgaaatacta tattcctgca tgagcagtaa tgcgtcggca
     1201 atttttttat ctgaagtaag agtgatcgga tttcttatca taccgctctc gcttcttttt
     1261 actttatcca cttcagcagc ctgcatctca gcagtcatgt tcttatgaat gattcccatt
     1321 ccgccttccc ttgcaactgc gattgccatc tcactttcag taacagtatc cattgctgct
     1381 gacagcagtg gaatatttaa aataatattt ttcgttagct ttgtcctcag atctacatct
     1441 ctaggcagta cgttcgaatg agcaggtttc aataatatat catcaaatgt aattgctgtt
     1501 tcggtaattt ttccagattt catttcaata aattgtttta acttttatca gatcattata
     1561 aatattttca ggtaagagat ctccgtcgta agatctgaca tacattatat gcagatgagt
     1621 aggagagcgt gacggataag cgttctttcc tgttctgcct aaagtaccta atggttttcc
     1681 gggctctaca aaatcaccaa cactgacata cacatcattc atatgagcat aataaaataa
     1741 tccattggta aaattatcat acacatatac aatgtttcct ccttttatat ccatcatgtc
     1801 agtggtccag ttttttcttg tttcaattac tatacctcct gtcattgaca atatttcaac
     1861 gaattcacct gtattatcat cgcgggaatc ctgatctctg tcatcaataa aaatatcatg
     1921 tgccggatgt ccgcctgtac tgccgtcaaa aaaatcaaat cctcctacta tgtagccgct
     1981 gccgtttctg ccgccgattg cattaggacc gtatcctctg acaggaaata tccattcatc
     2041 atcggaatat ttctttatga ctccagcatc aattctctta tccataaaat cccaaagctg
     2101 atttgtaaat aaattcaatg agtctattgc atcctgtctc gagaggttag cttttctgat
     2161 gtcattaata attacctgcc accttgatgc gattttatcc ggctttccgt aaaaattttc
     2221 atcagcttta agtccattta ttttcaaaga aattgaaatg atcgatgggt tgattcgttt
     2281 ggaaacaatc ttctccttct ttcctgattt ctttttcttt tgctttttat catctgcgtt
     2341 tacattattt gtaaaagcta aactaaataa aaaaataaaa gcaaatgtaa ctgctaagaa
     2401 agtttttatc ataattaaaa tattagaaaa attttagttt tctacaattt caatttcatc
     2461 tgtaacaatg acagttccgt caggctttaa tgcacttact ttgaatttgc cggttttatc
     2521 gaatttcaca ttgtcaaaat aaatgtaatc catatcggaa tcagtatcga acttaaaagt
     2581 ttccactaca tcacctgtat tcagatcagt tacattaatg ttaacagatt tcactcccac
     2641 cttggtttta gaaggtctga gatctaccat taccgtcaga ttaccctgtg tatattttga
     2701 acttttccct tcgcagttgt cagttgctga gctgtaagat tcacaaaagt aaagtttatt
     2761 tccgcctgct gaatctccta attcttttag tttactgcat ccaagtacca ggaataacaa
     2821 gacaaataat gctaagaccg gtttgtaata atttttcatg tttttaattt ttttttaaat
     2881 ttagccaata ctatttttat attccattac aaatttttag tgaaggaaat atattcacga
     2941 ggtattattt tcaaactatt ttacctgaag aattaattaa caggaagtga attaatttct
     3001 ttaactaaaa ttcacttcca taacaaaact tatagtccaa gtttatcttt tagaccgctt
     3061 gccatatcgg ctaatcctcc accaacatct tttcctgcaa gaaaatcgct gactttatct
     3121 ccgattcctg caggcagttt atcttttaca aaaccgccga ttgcttcaat tgctgacttt
     3181 gcctgatctg ctgtaattcc cgctttttct gaaactattt taattagatc atccatcttt
     3241 ctgaatcctt tctttttata ttaaataatt accggtttaa aaccagcttg attttttctt
     3301 tcgtgaacta ccgccaattc ccagcacgcc aagaaaactt cttgttactt cacgtgcaat
     3361 tgtccttcca acctgtctta ctgctgtact ttcaaggact ttttcgactg tgctcttttc
     3421 ttcttttgct gaactttttt tagtttcctc cactgtactt tccgctgctg catcatttaa
     3481 ctttttttcc aaaatttcat aagcactttc tctgtcgata acttcattgt atttttttac
     3541 cagctccgat ttacttacaa tttcttcctg ttcagcatcg gataaaatat ccatccgtga
     3601 agccggtgca caaagcagtg tagcaacaag cggcgtggga ttccctttct cactcaatac
     3661 agttacagcc gcctcgccta ttcccattga tgttaaaagt tcatcgacgt cataatattc
     3721 agaaatcgga taattttcag agatcatctt aatatccttt ctgtcttttg cagtaaaagc
     3781 tctgagcgaa tgctgaactt tcatcccgag ttggctgagc acttcatcag gaatatcagt
     3841 cggattctga gtgcagaaaa atataccgac acctttggat cttatgagtt tgataatagt
     3901 attgatctgt tcatgcaatg cttttgaagc ttcctgaaat aacagatgag cttcgtcaat
     3961 aaagatcact agctttggct ggtcgagatc accttcctcc tggaagttat tataaatttc
     4021 tacaagcaga caaagtaaaa atgttgaaaa taattttggt ttattctgaa tgtcagcaag
     4081 tcttataatg gaaactttcc ctcttccttc actgtcaatt ttcacaagat catctacttc
     4141 aaatgatctt tctccaaaga aaaggtttgt ttcattttca agttctatta gttttctgag
     4201 aatggtacca attgtcgctg tagatatctg accatattct ttttcgactt catctttacc
     4261 ttcatcagaa ataaactgca ataacttttt aaaatcttta atatccagta ttggaagttt
     4321 tttttcatca ctgtatttaa aaagaacaga tactacacct gactgagtgt catttaaatc
     4381 aagaattttt gaaaataaca caggtccgaa ttctgtgaca gtcgctctga gacgtgcacc
     4441 tttttcattt gagatcgaaa gaagttctac aggaaaagaa gaaggagtat aagtcattcc
     4501 aattttttca tgacgttcat ttatcttatc attaatttct cccggctgtg caagtccgct
     4561 caagtcacct tttatatcca ttaataaaac ggaaacactt tttgatgaaa gtccttcagt
     4621 aatcaattgc agcgtttttg tcttaccggt tcctgtcgca ccggcaatta gtccgtgacg
     4681 gttcatagtt ttcagaggta acttaacatg aatacctgga agattttctc ctttgaatat
     4741 tgctccgcct aaagtgatag tttcgccttt gaattcatat ccttcattca ctgaggcaat
     4801 aaatttttct ttttgatcca attaatttct tttaattgat tgaagtgaag tatttaaacg
     4861 catacaggaa actacaaaat tatactattt tttttaagtt aaataatact gcattttttg
     4921 tactttacca ccaagacacc aagacacaaa gaaaatactt ttttaaaata cgaaaactca
     4981 aaggctcaaa gactcaaaga atattttgcg aataaaaaga gatttatatt gggtctatgt
     5041 gaaaaatagt ggaattttta taattccttc ttttaatatt ctaattttgt tttttacgat
     5101 aaattttttg cttttaaaaa aacccgcttg tgtaaaatta ccgataaaaa tttatgagta
     5161 attgctaaaa taaaatttcc ttgtttgaga aatttgtgtt tttcaaattt cgagttggaa
     5221 attttatagc aattacgata taaatttcgg taattttaca tcgcgggcga cttttctttg
     5281 ttactttcga gcctgccctc gagtgctcca atcggggggt cgctcaaaag aaagtaagtt
     5341 aaaaccaatg aaacttgaaa tttaaatata aacgagttag aaatatttga aacttcgttc
     5401 cactataaat catatagcac ctttatattt aataacacta tatattttaa gtattagaaa
     5461 tttgaaattt gaaattcgta attcgtaatt tgcaattgct cttcagtccc tcgccattgc
     5521 cagtgagcag cacaaaacat ctcccgcgcc gtgctgttta agtatgttta ctgcttcatt
     5581 tattgttgcg ccggtcgtga tcacatcatc caataaaatt attttcatac cttttaatgt
     5641 atcagaatat ttttcattta acacaaatgc attttcgata ttgagccgtc tgtcggattt
     5701 attcagacgg gtctgagacg aagtgttctt tattctgatc aaaagatcat ctgcagtttt
     5761 tatccctgtg atattgctga atcccctgca cataagctgc gactgattga atcctctttc
     5821 cctttgtcgg gatctgtgaa gaggtacggg aatgatcata tcaaatttgg gaaattcatc
     5881 aatatctttt gctttcagaa attcaagacc gattatctca cccatatata tagcaagatt
     5941 tctcatgcct ccgtacttta tattgtaaat gatctttgaa aaatcatcct tttcataaaa
     6001 ggcaaacaaa ctgaatgaat attctgaaaa tactttattc ttcagatcaa ctttatcttt
     6061 atctgatatc ctaaaaagca gattgaattt atcatcctta atgtatctgt tggaattttc
     6121 attactaata tattcatcag acagtataca tgacggaggg taaatgaatt ccaatatgct
     6181 ttcatacatt cccgaaattt ccttcaacat tattttcaat atcactttca tacaattcaa
     6241 gatattaatc ctgaatcgtt ttttcaaata tgaatataac cggtttgtga aaatatcaga
     6301 aacttcaggt tgtctgaacc ttgattaaag gattaaaggg aattcttgaa taagaaccag
     6361 aaataaaatt tttaattcgg ttgattttaa aacgcactag atctgcagcg gtaaaaaata
     6421 aatcttgttt attaaaaaga aagaatatac ttttgcagtg accccaaaat taaaggagta
     6481 cgaaattttt aaaaggatta tgaaagaccc ttacttctaa aattatgtat tccccgttaa
     6541 aatatttata agaaatttaa tatgatttaa tttaaataga atcattcaaa atttttttta
     6601 aataaaaatt tttaattcat aaacagtaat tctaaattga ataagaaggg aggtaataca
     6661 agatgatcag agctccgaca aaaatcacca gagtgtccgc aaaaaaaagg gaccggaaat
     6721 atcaaaaata aattaactgt gcgactgcaa tcttacagaa aatataaatg acagatctga
     6781 tcccgaaatt ttatttacat taatttaact ccatttaata aaatgaaaaa gataaaatcc
     6841 gttccggtga aatatttatt agtattcatt ttctcagtta cattttttta ttcatgcagt
     6901 gatgatcctg tatctaatga tcctgtcgat cccacatacc agtatgattc agcacgattt
     6961 agctggaaag tagatacatt atatggtccg ggattttttg caggtctgtg ggcaaaagat
     7021 acaaatgata tatttattac aaatacattt agcagcagtc tttacaggat ccaaaatggt
     7081 gtaaagtctt atatcagcta tcctgtaaat acttatctag gggttgtaac aggtgatgaa
     7141 atgaataacg gatttgtcat tggtggaata ttaattgata cattagtaca accatttttt
     7201 caaaaatttg atggcaattc ttttatagaa attccaaatc cacaaaatct gaaacaaagt
     7261 ttaaccttta atgctgtatt tgtaaaaaat gaaaatgaga tgtggatagg tgcaaatgga
     7321 tacatattga aatttgatgg agtcaacctg tattcattcc aattagaaga ttcccggaca
     7381 aaagcattga aattttttta tgatgaattt aataatttca aattgctagc atcaattcat
     7441 catttagata ctgacagaac ggaatttata atatacgaat ataccggatc tcaatggata
     7501 aatatattta gtgatattga ttcaccacga cctcttcggt ataatatttt taacaaatat
     7561 gtctcagcag caaatctgaa tactatattt gaattaactg gtaatacact tactcctaaa
     7621 gtaacaataa ctccacccgg ctccggattt acgatggatg ggtattcatt taataatatt
     7681 ttagttccgg caagaaaata tactgacttt tgtcgaactt cattatttca ctggaatgga
     7741 ataaaatgga gtctggaatt atgtaattat tgggttgacc ctacaacaga tgtatatatg
     7801 ataaatgaaa attattatta cgttgtttca tataatcaaa atggaacaac ttttattatg
     7861 cagggaaaga aaaacaatta aaaattttaa aaaaaaatca ttatgaaaac aaatagaaat
     7921 gttcaatttt ttataacgct cattgctata ttttatatct ctaatatagc attatcacag
     7981 gagtgggtaa caaataatta tggaaatagt ggtcatagtt tagatttcct taatgctaat
     8041 acaggattta ttggtttgac agtttatgac aatggataca aatataaaat cttaaaaact
     8101 actaacaaag gtgtttcttt cacaccaata tggtcaactt cttcaaatca atataatcaa
     8161 caatcatttg cttttgatat gattaatgaa tatgtaggat atgtatatat atagacagaa
     8221 ctgttttcaa aacaacaaat ggtggaatta attggaatca gaaatttcaa attgaatatg
     8281 atgaatccgt atttccaact attaaatttg caaatgaaaa cttagggttt atatcttatt
     8341 cattaggagg atcaggagat agattaaaaa tctatagaac aattaatgga gggaataatt
     8401 ggagtgaagt ttatacaagc ccatcatatt ataattttgc accggtaata aaagatattt
     8461 caatttcaaa atcagatatt aataaaatta ttatggttgg atattatcat aattttcaag
     8521 aactagggca gattcgaaga tttactttag tatctgataa tgcaggtgca gattttggac
     8581 cggaatatga tggaggaatt tatggaacac aattttccaa tgttcaatat ttgcccgaaa
     8641 gttttactga ttatagaatt attggaattg aaaactacgg tgctctagat acacttaatg
     8701 ggactttttg ttatgataat ttttcagcaa caaataaata caaaataaca aatgatcatt
     8761 ttaacattgg cggtttaagt ttcattaatg caacaacagg gtattcttat gtaaatgata
     8821 aattatataa aagtaccaat tcaggggtta attggaatgt tatatatgct tttgcacaag
     8881 gttcacttaa atccaataga aatgctctaa cagcatttaa tgaaattatc tatacagttg
     8941 atgtgattgg aaatttagtt actcatacac tttcaacaaa cctatccact ttttatgaca
     9001 atatttctac tcccggttca ttcagctttg acaatgtatc ttacaataca ccgtcacagc
     9061 aatatctgag aggagggaat agcaaattaa attcatctct gattattaac ccaaatcaaa
     9121 gtgatgaaag aatattttac aaatggagtg atggtaatat gacaggaaat tatgatactg
     9181 actctaatca ttattacttt gatatgtcag gaacgactat tgctaattat tataaaacaa
     9241 aacagctttc aacatcaagc gaagcgattt caaatattgg tcagaccaaa gttctaaaag
     9301 ataattgggg agctataaac caaattcatc aaagtatgaa tggtggaata ttttacacta
     9361 agtcttataa taatggtaat aattataaac aggaagaaat tataaattct agtataccac
     9421 atgctattgc agatggaaat aaaaatcctg atctatatga gacaaaatgg tatggcacaa
     9481 tagcgggcgg ttatccggtc aataaaaata tgacagcatg ttgggaaaga tataacccat
     9541 caactgagaa gacggagatc aaaacagcag ttagagatta ttatattgct tcagatactg
     9601 tatttatgtg ggatgcctgg gaggatgcaa attattctga tgtatttact tcatttaatt
     9661 catctccaag ctataattca aaacccaaaa tatttgccct tgctatggat cccggaaata
     9721 atcttggtaa tcctttaact tattttttta taattccaca tttacgtcca tattcgggtg
     9781 gaaataaatt acaggtcacc tgccgaaaag gaacagatta ttatgcagaa ttttcattgg
     9841 attccggtga catttctgat ttctctgcaa tagattcaat aaagtgcagc cttggttatt
     9901 tagaaattaa ttttacatat cgaaagggaa atcagatatt atatagaaat attgcatttg
     9961 aatatcaact taatttgcca attcgtaaac ttgatttgga aggaccaatt gatgtctctt
    10021 ccggtgacgg atattcttcc agattcaaac ctgatatttc attaatgaat aatgtacctg
    10081 ttgtaagtta cgcagcaagt tttaatgcca atgtatatgt agaatatgaa ggaggcggat
    10141 cagacttagt tcctgtcagc agatatccta ttgtgaaagt gcagagaata aataatacaa
    10201 gctggagtaa ttatgtaatt tacaattcca attctgtaca gcagacgccg gatatagaag
    10261 gcagtaattc tactttgtca tacattataa attattcttt aggcaacggt cagtttaaga
    10321 aagttgtaaa tgtaagtgat tatcccggat atttgtgtca accgaatact ttttcgggaa
    10381 ccgattcaag acttgtgaaa aattcttatt cgggacactt tggaaataac ttatctcttc
    10441 tcacgctttc acctcaaaac tctttgcaaa aagtagataa gcagaatttt ataattacaa
    10501 atatcacttc ttcagacagc tatgatgtgg ataatataga tggtgtggtt aatcttgata
    10561 caataaaata ttcattcaag ctcggaccaa ttttaatcaa agaaccaaac ggtgaagtag
    10621 aaggaggaac atttggaata gagcaagctg aaaatgaaca gattctgaca ggagtggaat
    10681 tcaatgaaaa tcttaagtca tatccatttt tactgaatga aactcaggca ttactattag
    10741 gatgcaatgc tacatacatc aaagacaacg cttattcatc aatcagcagc gtaccttata
    10801 cagtaaatct ttataacaaa tctacgggac ttcttcacag gacattattt caggatacaa
    10861 tacacggaga ggattctata gagacggaat ttctcagagg attttttata acaaatatcc
    10921 caaacggaga agacagtttt tatgtaaaat tagaacctga taattctttt gatgacggaa
    10981 atttctatat taaccctgtt tatgatgtag aggataatgg agacaatcca aacagttcta
    11041 atgtcgcagt gatttttgaa gacaataaat tcgaaagtca ttctgaaaac attccgcagg
    11101 acttttcgct tcatcaaaat tttccaaatc cgtttaatcc atccactgtg ataaaatatg
    11161 atcttccaaa ggaaggattt gtgaaaatta aaatatatga tctgactggc agagagatcc
    11221 ggtcacttgt aaatgaagtc aagcaggcgg gatcatacag tacagtcttt aatggagcaa
    11281 atctttcaag cggagtttat ttttataaac tagagaccga aaacttcaga cagataaaac
    11341 gtatggtatt gctcaagtaa attaagactt ttaaaaatat ttatcttaag ccggattgag
    11401 tgatttgatc cggcttttgt taagcggatt aaaattaaaa attaaaatta tatatgtaaa
    11461 ctcttttata ttcaacctta tttgagaatc aaaaaaaaac ttccgaagca attttaactc
    11521 cggaagttat tttcttacaa tttataattt ataattactt aatgaatgcc attttcttaa
    11581 tatcagtaaa accatctgat tcaagtttgt agaaataaat tccgctgttc atattaatgg
    11641 aattataatc atacctgtaa tttccggcag ctttaatttc atttaccaat acttcgactt
    11701 ctctgcctgt tatatcatac actttcaatt tcaccagtcc gctttttgga atagagaaat
    11761 taattacagt cgatggatta aacggattcg gataattctg cgaaagagaa aacttatccg
    11821 gtatctctgt tgatataatt gaaaaattag tgaggattcc gccggtagta gtttttaaga
    11881 ttatgccgct gtcgccgacg acccatcctg taagagagtc agtgaaatag attgaattga
    11941 atgttgttcc tagttttgga tttgtttgtg tattccagct tatcccgcca tttgtagttg
    12001 cttctataat tttatattct cctgctgccc acccataatt attattaata aaaaaaattg
    12061 aattcatacg ataaccggat accggaaaaa tttctggaat ccagttgtca ccaccgtttg
    12121 ttgtcctcaa aataccttta ataccaaatg gaattgaact ggatataaag ccatttagtg
    12181 aatcaagaaa aaataaagaa taaatattat catttgtaaa aaaggaaaaa gaccaatttg
    12241 atcctgaatc aattgtcttg attaatgccg ctcctcctaa tgccggcgga agtcttactg
    12301 cccaacctat ttgtgaactg acaaagtgca cacgagttat agtaaactcg gtttggactt
    12361 caattgaatc ccaactatat ccaaaatcaa tactcttata tattttattg gttggattgt
    12421 cattatctag attacttccg ccggcaaatc ctgttgagtc atttataaaa tttagggaag
    12481 ttaaattatg ataagaaatt aaacttgttc tattccagct ttttccgcca tttgtagttt
    12541 taattattaa tcctgaatct cctgcaatgt atccggtagt taaagatgta aaacacattg
    12601 aatttagatt aaaattaaat ccgctgtttt gcttttccca agtaaaacca ccatttgtag
    12661 tatttaaaat tatacctccg ttccctatac accaacctgt taaagagttt ataaattgaa
    12721 cggaatttaa agaactatcc gttcctgaat ttagatttgt ccagccggat tgaggaatac
    12781 aaactttgct gtcaataaaa aataaaaata ttaaaattcc tattttcata attttaatta
    12841 aaaaattcta ttaatattaa tctaaaagtg gatctattat tgtctgataa ttatatccca
    12901 ggtccatagg atctatacca acaccaactt tttgaattat tttagtttgg tcaattccag
    12961 aattcccaaa ccatcccaaa ttaaatacat ctatagtatt atttcctata aatatattac
    13021 tgttgacgtc actgccagaa ataattttta aattatttag tgtaaagatg gggtaaatat
    13081 tatttataaa tatccccttt gcagaagtgg actgactcat actttttatt tttgcatttc
    13141 taataaaaaa atttcctcgg ttactcacac ttccatttga aaaaattcca gctgcaccac
    13201 caaactcgat tgagttaaca tcaattgtta tctgttcgtt agtatcattc aaaaccaatg
    13261 ctacatgatt tagacttgta acattatcac attttaaata accttttgta ttagctcctc
    13321 caatttgaat tgccgctcca tctaaagaag atataatttc tgagtaaact cgtcttccga
    13381 taaaaattcc tgtaccaata gtacattcaa ttccgcaaag agcattattc gtagctccgt
    13441 taaaacaatt aatctcgtca aaatagagta tcaatttttg acctgctgac ccttgatttg
    13501 ctgctagatg tactgttgaa acatttcctc cggatctatt cattatagat gtcatttttt
    13561 taattcttgc agttattgtt ccttcctgat gcgacagaca atgtccaaga ttgttacaaa
    13621 tgatttcatc aattcttaca aaaccatttc cccgggtaga taaggctgta gtaccagtag
    13681 aactattatt tacatcgcct gtttccactt tagtgacatc taatcttact tcctctacca
    13741 ttccaggttc agcgaattta ccaatacgaa tagcctgaag attctgacta taaacataat
    13801 tacattttaa atggaattta agtgatggag aaatattaac tgcaacacca tttacatttt
    13861 gtatataatc acattcaact gataatttag atcccggatt ttcgactctt atacagctgc
    13921 ttgatccagt atttttaatt accccataac cataaatcgt acactctaca gtttcattat
    13981 catctgtaat agtctcaccc ggattggatg gatcttgatc aatcactacc cccggcatta
    14041 tccaaatatc cactttatct ttaagtataa tctgctcagt cagatcagca aggatctgaa
    14101 tcagatcatg atctccagga ttatttccag aggcagttct tgctgcgtta taagttgagt
    14161 aaaatgttac aacaccgttt ttgtcaattt tggaaacttt atcagccata atttaaaatt
    14221 aattttttta aaaaagatac tttagacaaa attttatttc aaaagtacca tacgttttgt
    14281 atcaattata ttcccatcaa tctctaatct gtaaagataa accccgctcg gataattcgc
    14341 tccgtcaaat tcaatttgat aatttcctgc gggtttattt tcattttcca aagtagtgac
    14401 ttcattccca agcacatcac tcacaattaa ctttgtaaaa ctcgaaaccc gtaactcgta
    14461 actgataaat gtcttcggat tgaatggatt tggataattc tgcgaaagat tatattcaga
    14521 tggcgttaca aaagaaattg gattaacacc tgtgagtaaa gaatatttaa ttgtcagaat
    14581 atctctgttg ctgcttcctg taacgtatac acttccatta ttatctacgg ctaaagcagt
    14641 tgcaaaatct gaaaattgag ctgagtagtc atatcttttt acccattttt gttgtcctga
    14701 tgagtcgtat ttaactgtag cgtagtcatc acttgttccg tttccatcac tctcaccggt
    14761 tacatatacg tttcccgtat cgtctacatc catagcaaat gctatgtcat ttagtccatt
    14821 atggtaacga gttatccata atatatcacc atttgtatta tatttaatcg tagtgtaatc
    14881 atatcctgtt cctgactcag tactatttcc agttacatat acattcccta tgctatctac
    14941 gatcatagct cttaactggt cttgaaaatt agaggaacca ttatatcttc tgacccattg
    15001 ctcttcacca tctgaattgt atttgactgt tgcatagtca taatagaaca tgtcacctgt
    15061 gctgctacct gaaacgtata catttcctaa attgtcaact tctatagatc ttgcagcatc
    15121 attaccgttc attggtccat tataaaaaac agcccattgt tgaaaaccca atttattata
    15181 ttttattgtc ataaaatctc catcactcct tcctgttaga tacacattga aagaatcatc
    15241 aacagtaaca tcaaatcctt ggttagagga atttaatcct ctattaaagt tattgatcca
    15301 taattgattt ccgacagaat catattttaa agtgaggcag tcaatcccac caatattatt
    15361 tttagattct cccgtaacat atacattccc aatattatca attgaaagtg aataagaagc
    15421 atctcttcca tttccgttac ttgaatatct tcttacccat tgtaagtaac ctgaattact
    15481 aaactttaca gtagtaaaat cagatccggt attaatacct gtgctagatc cggttgcata
    15541 tatattgcaa ctaccatcaa ttgcaatttc atcgatgccg tcataactat ttcccggacc
    15601 attgtaaatc gcagtccatt tttgcattcc tgaaggatca tattttatta taatgaaatt
    15661 tgtgtttgag ccagaaccct cagtacctcc aatataaata cttcctgtat tgtcaatagc
    15721 tattgaattt cccgttcctg aattgaaccg cttagaccat tccattgaaa cttgagaatt
    15781 cataaaactc gggtacagga aaaattgaaa aacaaacgtg aatatagaaa taaacttaat
    15841 cataggtcag cttacatcca tgctaactat atatttgaaa ttccctgtaa ttccagtacc
    15901 aatcttaagg gttatattag acgaaatatc cttttttaca aataaagtta aattcttgat
    15961 atcgatgttc tgatttccat ccctataaat actagcatca ttactaccag ttccagtcac
    16021 tattataaga ttttcaatct ctatagtttg gtcatttgct tggttattat cagaaataaa
    16081 aattcctctg gaaaatggag ctgaacttcc agtgtaagta tttattattt tcgcattcct
    16141 tataacataa ttcgaaccgg ttgaagatct aatcactcca tcattaccgt tacttccttc
    16201 tattttattg gaatctataa taattttctg tgtacttgta ttatgaatgt ttattccttt
    16261 tgtcacactt ataatttcat ctactaatat atttgcatcc gaagctaaat ccaatgataa
    16321 gccgtctgta gaaaatatcc ttctgccaat aagattaatg tttccataat ttgctgatac
    16381 ggcatctccg ccattaatat ttaaattttg aatttcatca aaatataatg ttaggtcctg
    16441 ccctcctgaa tcgccggaca tccttactgt ccctgcagaa gcattacccg aactttgtgt
    16501 tgtgagtttt agtacatttg cagttaatga tcctcctcta aaaaacagac agcttgcatc
    16561 atttttgcag attatttcat ttgcggtaag aaaacctatt ccgcggaatg aaacagcatc
    16621 tccgccggaa taatctccgc tctctattat atttgctctt atatttacat aaggtacctc
    16681 atcttcaaaa taaattgctc tttgttttga atttactatt ttatcgcagt tcaaataaaa
    16741 ttttgagcaa ctttctatat atactgttgt accatcaaga gaggtcggat cagatccctc
    16801 attttccaaa ttatcacatg taattgaaac ttttgaatct ttgtttataa tttttacgca
    16861 tccgtacttt tcatttgtat ttttaaaaaa tccattccct gatatattta cttctacggc
    16921 agatgtatat cctccgtcat tgtcaagtat tatcggagat gagtctgtca tttgaatgat
    16981 cctgccggga gctatccaaa tatccacttt atcttttagt aagagctgtt catttgtcag
    17041 atcagcccag atttgaattt tatcatatgc tgtagctgct gtccgtgcag cagcataagt
    17101 tgaataaaaa aattgagctc cagttgctgc agttattact gcgacttcgg ttgccatgat
    17161 ttaatttttt ttgattaata aatttagttt attaagttca aaatatgagt aaggagggag
    17221 ggggtcagaa aaaacctcat atttaattcc ttttaatatt attgatttaa agtaaatagt
    17281 ataatttata attacaagga tttatttgga aaaaagtact ttacggagac ttgactcctg
    17341 atgctgtaaa atgtaagcag caatataaac cgctactcct catctataaa aaataaatcc
    17401 cttgtaaaat ataatccgca aaattaattt atagccggtc ttaaaattat atgattattc
    17461 tattatgttt agttgcgaac ttcgtcgaca ctgttgttgg tgatcaaaaa gtttgcacaa
    17521 gtggaggata taaattgaag attaattaat gcaaactttt tgaaaacacc agcaacggcg
    17581 gaaaatatga tcttccaaag gaaggatttg tgaaaattaa aatatatgat ctgacagaaa
    17641 gagagatccg gtcacttgta aatgaattca aacaggccgg ttcgtacagt acggtcttta
    17701 atggagcaaa tctctcaagc ggagtttatt tttataaatt agagaccgaa aatttcagac
    17761 agataaaacg tatggtattg ctcaagtaaa ttaagacttt taaaaatatt tatcttaagc
    17821 cggattgagt gatttgatcc ggcttttgtt aagcggatta aaattaaaaa ttaaaattat
    17881 atatgtaaac tcttttatat tcaaccttat ttgagaatca aaaaaaaact tccgaagcaa
    17941 ttttaactcc ggaagttatt ttcttacaat ttataattta taattactta atgaatgcca
    18001 ttttcttaat atcagtaaaa ccatctgatt caagtttgta gaaataaatt ccgctgttca
    18061 tattaatgga attataatca tacctgtaat ttccggcagc tttaatttca tttaccaata
    18121 cttcgacttc tctgcctgtt atatcataca ctttcaattt caccagtccg ctttttggaa
    18181 tagagaaatt aattacagtc gatggattaa acggattcgg ataattttgt aaaagtgaaa
    18241 aaccgtcagg tattaaactg cttatttgat taattcctat ttcactaata ttgattggtg
    18301 ctgtccatat ttgatatttt ccggtaaaat cagccatcca gaccggccag agttttttac
    18361 cgatggaagt gatcccaata ttatcaccct gatttccgga tcctacagca ccggttactg
    18421 tctttggtac aaatctgtga tcgtttattc tggtatctgt ccagcttgtt cccccatctg
    18481 tagatcgcga taaataaacg tccatggaat ccgaacttat atttctgttg tcataatata
    18541 ttacatttat ccctccgaag tcatcgacat ttatcgcagg gaagtactga actttcccgt
    18601 tatttatcgg atcctgattt actctcacac ctgaagacca ggtaccgccg ccgtcagtgg
    18661 atctgtgaaa tacaatatca gcatcgcttc cggcaggaga tagatttttc tcccctgtaa
    18721 caatatagat acagccgttc ctttgacctc ctgttttgtc cagatcaata tgcggatatc
    18781 catttacgcg gatattccac ggctgaaaag aagcagcttt tattccattc attgcataga
    18841 tattttcatt aacgttccag gtatttcctc cgtcagaaga tcttgcgaag ccgcagaatt
    18901 tttcattctg tggagatgat gtcaaaacac ccgcccagca gacacatact tcaccgctta
    18961 catttacttt gatatctgca ccaagtgaaa cgtaaccctg cggagatgca ttgatctgaa
    19021 ctgctgaaga ccagttaagt ccgccattag ttgtataaga ggaaacaatt ggaaatggat
    19081 taagaaattt tgtccagaca agatagcttc ttccataaaa tggactagtt gaaatatcat
    19141 cagtacccgg agtgccttta tcctgatcac ccgaagcaat taaaacattg cttgtccagc
    19201 ttgctccaaa attagttgaa agatttgaga acatcccgac tataaatccg ccctgatgtg
    19261 taagaataaa tctaccgtcc ttgtcaatta taggacccgg atcgccacca tgattatttg
    19321 ttgaagaacc tgaattacag gtatcagatc caaaccatgt ttcaccaccg tcagttgtaa
    19381 aataatttcc ctcacttctg aatgaattgc tgattgtata agcgactgca aacattacat
    19441 tcggatctac aggactcctt actatggccg gttctatctg tgcatttcct gaaggatgta
    19501 ttctgaaatt tgttatttga ggatcagctc ctttgttaaa aataaaaaca aataaaataa
    19561 ttaccaataa aatttttaca ttcatatcaa tttttaaatt taaactgcaa ttttttcttt
    19621 ttagaaaata ataaatgatc agaatttata accgaaatta aatcctaaat aataatttct
    19681 cggttcaccg gtttcataat atcttctttc cgatggattc agttcagggt ttgcatttac
    19741 atttacaaag cccacatact tttcatcgaa aatatttttt ataccgctgg ttaaaataca
    19801 gtaaaagtca ctgatattca tagtgactcc tgccattatg ttaccataaa aataagaagg
    19861 cacaactaca gtatttgcat cgtcagcaaa catatcactt atataatcca tatcccataa
    19921 caacatacct gaatacttgt ctgaaatatt gaattgataa tttaaaataa aattaaaaat
    19981 actattcgga attgcaggga cgacattatt agaatagtct gaaacaagtg tatctccgcc
    20041 ggcagaataa ttcacagcaa aataatcatc atatttgaaa tctgaatatg aataattcac
    20101 caccagttca attcccctta gcatctcaaa tttaaatcc
//
LOCUS       DAORVQ010000040        19851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1092_length_19851_cov_7.016064, whole genome shotgun sequence.
ACCESSION   DAORVQ010000040 DAORVQ010000000
VERSION     DAORVQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 19851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19851
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_19851_cov_7.016064"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2367
                     /locus_tag="PLX80_05120"
     CDS             <1..2367
                     /locus_tag="PLX80_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY20209.1"
                     /translation="YSATFNGFNSYVAVPNSLGLSPTGGITLEAWVNPSQLGPGTMSV
                     IGKNYQTSYFIGLQVSGRVVFYPKGGASLRSKVTGVIPVNQWTHIAGTYNGTSTAIYI
                     NGVLDTITYVNTGPVTTNSDSLFIGADRVGAATSLFFRGKLDNVRIWGDAKGLSQINH
                     NMFTPLEITFPTGEYINLRASFQLNSNGLNYGGSELNIGHTRNISFTDISSESVNYLD
                     YNNSLVLNGTTDYFTVSNHAGFNATNAVTLEAWIRRDTTGTQPNDQYIVNKSGGTNRF
                     GYALWLNSSTGILKFKINSVAGPSGIVSLGAIQTAQWTHVAATYNSATGIARLYINGK
                     YSNGVFFSGSPLIQNDPDNLFIGGIGATDLAAYKFKGQIDGVRIWKTERSLNQINDNM
                     YNNLSDINMVSFDFDEKSSAVHNGNTTSYNSTVFAGSAHISSSHLNNNNELTSPVLSN
                     ELGNFNSSQYTVSTKRTYIPDGYVSGIWDSVYIPANGSITDIKLFTLISHKYTSDLDI
                     TLTSPSGNAIMLIGSKGSYGNDIMTLFSDGAGNYGSGGVGVNDPGITPPFSPSIRPAG
                     AFSIFDGENMQGWWKLNCSDKSSGNIGYVHGWGLNISSVPLHKVLILNALIQGFYDMN
                     SNEMVQDYVKANLRQVNSPYSIIDSSVTIFNQNGVGKFNFSNITNATEFYIQTDHRNS
                     VNTWTSGSNIFNSDTLYYNFTLAAGQAFGANQIKVDNSPVRYAIYNGDVNKDEIIDLT
                     DISLVDNDAANFMTGYVLTDLTGDQFIDLNDLTIADNNAFNFISVIKP"
     gene            2479..3318
                     /locus_tag="PLX80_05125"
     CDS             2479..3318
                     /locus_tag="PLX80_05125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20210.1"
                     /translation="MKQIRDQQKDLWNKFSPGWKKWDDLTMEFLEPMGIEIVRLIAPK
                     EKELILDVASGTGEPGLTIANMIREGNGKVIATDISNGMLEIACENANKRGINNFETI
                     ISDVSDLPFEDNTFDAISCRFGFMFFPGMLQAAKEMVRVLKPGGRIATSVWGAPEKNF
                     WITTAMKTLKKNMDLPEVPKDAPGMFRCAEIGLMAEIFKQAGMKNISEKEVSSKLNTG
                     TAEAYWNFLNDVVAPVVSAMSQANDSIKNKIKDEVFDLVNQKYPEGNVAIDSSAYVVY
                     GEK"
     gene            3971..6562
                     /locus_tag="PLX80_05130"
     CDS             3971..6562
                     /locus_tag="PLX80_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20211.1"
                     /translation="MAKAKQKGKSTVSSLKTKAHDTSEGFTNYDNYIYIALIILIVLF
                     FFREGVFGGKIFATSDNIASESFRTFLDDAKKNGIFPLWVPYIFMGMPNFPIISYNPR
                     VYDFFYFLWDSGYGLLTNSSENPVLPIVMYYVVFGIGFYLYAKYKFKNKLIALYCALS
                     ATFATDFIQRIVVGHNTKVLSLAFFPLILLALDKLIDSFSDKTKSLFSFQNLLNLFFL
                     TIMLHIQLNSNHIQMIFYCYLMLGIYVGYLIIYNLVKKTNVSGMIKAGAFFIVAAVIA
                     LAMNADVLMTMKEYNKYSMRGEAGILAKLDTKVADDQPLDYEYATNWSFSPGEVITFV
                     LPYYYGFGDVQVQGQKANLYWGQMPFTTNPMYFGVVTLVLCLIGIIYNFKRNAAVQAM
                     TVIAVLFLFLSFGRNFSLIFDMFYNFFPYFSSFRAPVMIHHFMNIAIVILAGFGLKSI
                     VDIVKEGLHLEKFKKMCYVFFGIAGLMFLISIIGFENSYTDSISNSSLIKKLQQQGAN
                     AQQISQYVSQISTVAYENIVSDLRLHAFFIAIVIGLALMYSQKSLSLKMFLIGVVLVG
                     LIDLWNVNSKTLHWDNPGDNNSAFGDTDYTNWLLKNAPDTYTYRVAEMNGGRLSTNNS
                     MSYYRIHSFNGYHGAKIRIYQDAVDVAGDNNPMLLSLGGVKYIITDNPIQDSSYTQVF
                     KGSRLIYENNNFIPLVFFADEVKVESGINILNNIRDLNFNPLQTAYVEKEIPQKIDKP
                     DSTVSAKLVKGEIHSLEYEVNASGNNLLVFSEIYLPFGWKAYIDGSPAEFYKTDYFLR
                     SLIVPQGKHKVEFRYEPEVYYAGKNISMASNGLLGVILIAGIIGYATRKKKNDDRKTE
                     KPENVNN"
     gene            6566..6994
                     /locus_tag="PLX80_05135"
     CDS             6566..6994
                     /locus_tag="PLX80_05135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY20212.1"
                     /translation="MITTENKKVKIIADVTLLQEGKVLMVKYSDKNKYDHQSGWFLPD
                     DLVSHGEHPEDAASRILNEQLGIIDSAPVFDHIESFTGNDGSWHLVFHFKYKAENLPD
                     VVKSEDVDSMEWFSLNALPPKEEVAHHGWALYTLAEMNIK"
     gene            complement(7084..9531)
                     /locus_tag="PLX80_05140"
     CDS             complement(7084..9531)
                     /locus_tag="PLX80_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQY20213.1"
                     /translation="MAQSDIRIANLNIKNQTTEEFTNSIYKFPFKVSISFNKLIEHIE
                     AAAECTNDIKKEFAANILTEINKNPGIKETFNEIKDVKPYEKLISTMMLFVFPDAFWE
                     KQTYALTTVFKKEEFFTSPKYREIIMNGGEVLDGDLNIDEVSFNFGRIVGGYAYILNY
                     VYGFDFKFEIPIVYKIPHPETGLDRYFKLNMANEFADVIVKGKLRKFTEEERKEIYRR
                     IYDIEFLKKIIPPEDYEYTGFIVVNAINITDTEILSAIKKDLIDKHTVSMQTSFLKLQ
                     HNIRSLLRESSVTMGIAPIDSEKDLMFLHGNRVLNNFQLSCFCDLNTDRAGSVYEMCV
                     KEKKVLIIDDIEDYPEATPTEKHILKNGYRNLMVAPLIFENKVIAVLELASQEPSVIN
                     TFNTLKLTEIYPLLGLSIARFKDEFDKRIQSVIKEKCTAIHPSIEWKFQNAAVSYLLA
                     NDNELNADGKQEIEMEEIIVKNVYPLYALSDIRNSSVIRNDSIKNDLIENLEMINDIL
                     NESFNFKPLYILKNLIYRIEKKIKSLNFAINSGDENNIVEFVNSEVYPLFDIIGSFDK
                     KVSDKIENFRKEIDLDFGIIYKRRKDFEKSSTALVSMITTYLDSEQEKIQKVYPHYFE
                     RYKTDGVEHSIYIGAALNEDGHFDNLYLKNIRLWQLILTCGIICKVKELENELSIPLQ
                     TAQLILVQNSPLTIRFRYDEKRFDVDGTYNVRYEIMKKRIDKAEIKGTEERLTQPGKI
                     AVVYSQTSEEKEYLRYIDYLQSEKYLTKEIEKFDLSEMQGLSGMKALRVTVNTESKET
                     GQKIDTEKLLKVAERIN"
     gene            9914..11155
                     /locus_tag="PLX80_05145"
     CDS             9914..11155
                     /locus_tag="PLX80_05145"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20214.1"
                     /translation="MKKIIILVFLYLFSVLNSEAQWIQQNSGTNQNLYDIEFINEKTG
                     WAVGDAGVVIKTTNGGTNWLYLPNPSPLISPNLWSVCPIDENIVYVTSSGDFIMKTTN
                     GGLNWDVLHSCPSCNSSTKGIYFLNKDTGWVLGAYKVFRTYDGGITLDSFYVPWFTNF
                     DIYFKDINTGIFCGTGRVFKSTDKGENWIDTHIPVGGSFPMFRKLGFSGNNIWLGGGN
                     PKIYKSTDLGENWIISDSSIGADGIGFVNDSIGFIGGGLNTLYKTTNGGYNFYRQKTD
                     PSSLAFISSIAFVNELTGWYCCAVGRIYKTTNGGEWLTNINSQFEELSSEDFKLLQNY
                     PNPFNPSTYIEYQLFKQGFVELDVFNITGKIISTIVNENKSPGNYKVKFESRGIPSGI
                     YFYNLKIDGKLVNVKKMIILK"
     gene            11181..12446
                     /locus_tag="PLX80_05150"
     CDS             11181..12446
                     /locus_tag="PLX80_05150"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20215.1"
                     /translation="MKKIILIFTIILLLNQGAEAQWILQSSFTNNNLYDIEFYNRNTG
                     WAVGDGGTILKTTNGGTNWNNVPNPAVGKPLSSIHIVDSNVCYVVGWFETIIKTTDGG
                     TSWIVIRNNGWGFGSSYDAVFFIDENTGWIAGTGQKILRTTNGCDSIITDYLFTGNLH
                     DMYFKDALTGIVTGSSLGSTFKTTNGGLNWFELNIQFGFTVSTFYKLSFINDQTGWIA
                     GSDKRVYKTTDFGDTWDTISRIPISQNSISMRFAEFIDENTGFVGGEQGYLFKSTNGG
                     YNFQRQNTSSFPPNEIISMHLYSDSIGWMVGGEAKIFSTTNGGQILVNINNSNLNELN
                     KLALFQNYPNPFNSSTKIEFSLAKNSLAEIKIYDIQGKEIKSLLKKTLTAGNHKINFD
                     ASGLYSGVYFYSLIINGVNTDMKKMIYLK"
     gene            12586..13200
                     /locus_tag="PLX80_05155"
     CDS             12586..13200
                     /locus_tag="PLX80_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQY20216.1"
                     /translation="MKSNTYRSLVIMFVVLFSTSFYGCGYNTLVTMEEGVNSAWSQVE
                     NQYQRRADLIPNLVSTVQGSADFEKSVLTEVTDARSRVGQVQLNADDLSDPQKFQMFQ
                     QAQDQLSSALSRLLVVVESYPVIKSNENFLQLQSQLEGTENRISVERKRYNEAVQAYN
                     TETRSFPTMIMAKIFGFKEKEYFKSTAGSENSPDVNFDFKKKEK"
     gene            13238..15679
                     /locus_tag="PLX80_05160"
     CDS             13238..15679
                     /locus_tag="PLX80_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQY20217.1"
                     /translation="MQAVIMAGGFGTRLRPLTNNIPKPMVPIANKPILEHIINLLKTH
                     SVKDFVVLLFFMPEVIREELGDGSKYGVRIRYVVPDQDFGTAGAVKLAEQYIKGKFIV
                     ISGDVLTDFDLTAISDFHKKKKTIATLALYRSKNPLQFGIVLTDKNDKIVRFLEKPSS
                     SEVFSDTINTGIYFFNKEIFKHIPPGENFDFSKDLFPSLLDKNIPLYGHKTDGYWRDV
                     GNLEEYMDANLDVLSGKLSYIKAAGKDGNCISKTSKIDKDAVIENSIIGENVIIEKDV
                     IIRNTVLWKNIRICSDSRLLFDVVGSNCYIGRGTRINDYVFIGENCVIGNNVFISSSL
                     KIWDNKQIENNQKVTRSLIYDDKFFADLFTDSRITGLSNLQINPEFGAKLGSVYGAFI
                     GQGKSIIIARDSDYISNMIKRAITSGLMSSGVNVIDSQVVPIPMLRQELKSGEGSGGI
                     FVRKSPFDGRSSDIIFFDKDGKDLSGNKTKSIERLFFSEEYKRADFEKVGSIKFQERS
                     NEKYTKHFLECLDLPEIRKKKFKIVIDYSYGIASTIFPNILGEFDCDVVSLSAHLDSK
                     KVTRPLEEYHSSQEHFTYVVKSLKYDFGFMIDAGGEKIWMSTAEGKLLEGDRFLVIVL
                     KMFLMVTKNVKKIAVPVQATGEIDLVAKEFGVEVIRVKNTHYSMMMACDDSEVKFVGG
                     TRGGFLFPEFLYATDGIFSVAKIIELIARSGVSIDDLHRTTAGLHLLKENIECAREDK
                     GSIMRKFMEDNTKYRQELIDGVKIFIDKNTTVLCIPDSDRNLIHLNVESNKLSVAKKY
                     HKEYKKKIEKYLSKK"
     gene            15709..16179
                     /locus_tag="PLX80_05165"
     CDS             15709..16179
                     /locus_tag="PLX80_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQY20218.1"
                     /translation="MNISEILDEKVIRVDFECKDKEDAINKMIELASHSGLMIDKEKV
                     RECVFQREELVSTGVGKGFAIPHGKTDEIKDIVAAFAVLKDPIDFDSIDLEPVRFIFL
                     IIGKETLLNAHIKLLSRISRLMNKDEFRDRLEETKSSEEVLKLFREEEQSVFDI"
     gene            16269..17993
                     /locus_tag="PLX80_05170"
     CDS             16269..17993
                     /locus_tag="PLX80_05170"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HQY20219.1"
                     /translation="MYKEILYNISFPDRQVHYCEVGIILTDPKESESLFYMPVWTPGS
                     YLIREFSKNTEDIKAVSSDGEELFTEKINKNSWRVFNPDKKNINFSYRVYCNELTVRT
                     SRINSDHAFLSPAGIFMTVEGMESVKCIVKLDLPDEWKEISTGLKNISENIYYADNYD
                     ILIDSPLEIGNHNILEFEIQGVKHFISLTGSGNYDGVKLIHDFKKIAEEQINFFGGEI
                     PYEHYTFIIHLVENGGGGLEHRNSFVVQMVRLNFCDKKLYNKFLGLVSHEFFHLWNVK
                     RIRPAELGPFNYNEENYTKGLWVAEGWTSFYDNVFLRRCGLINDEEYFEFLGHEYNDI
                     MRYSGRHHQSLTDSSFDTWIKFYRKDENYNNSQISYYTKGALVAMMLDLEIIRNTNAE
                     RSLDDVMRILFDDYKKDNTKGFTQERVKEICEFVSGINFDDFWKKYVNGKDEIPMEEY
                     ISLAGLEVEDIADTASSFLGIESLTQEGRLIISKVFTGGSAYISGLNFKDEIISIDSY
                     RADEKIAKNILSGKKPGDVIKVMISRDGIVKEINVMLAKPFQLFKIKRMEAISSDQER
                     IFYKWING"
     gene            18064..19626
                     /locus_tag="PLX80_05175"
     CDS             18064..19626
                     /locus_tag="PLX80_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20220.1"
                     /translation="MKTKYIFTIIMMIFTAGFTSAQYQNIFISSTGSPNEPAICMNPK
                     NTDQIVAGSNLNFYYYSTNGGYNWTRATLTSTLSVWGDPSIAVDTAGHFYYGHLTNPQ
                     GSYFIDRIVVQKSTNGGMNWNNGAFTGYVPPKQQDKEWICVDPRNNNLYMTWTQFDAY
                     GSSSPSDSSAILFSRSTNGGESWSDAVRINKIAGNCIDNGNTVEGAVPTVGPNGEIYV
                     AWSGPLGIVMDRSTDEGLTWLDEEIFVTTQPGGWDYAIPGISRANGLPVTICDLSNGP
                     DRGTIYINWSDQRNGTTDTDVFLIKSTDGGNTWGQVKRVNDDPPGKQQFFTWLTIDQK
                     NGNLYFVFYDRRNYANNQTDVYMGRSTDGGETISNFKVSETPFNPSSSVFFGDYTNIT
                     AYDGKVRPIWARADGTSMSIYTAIVDFITNVEQINTIKPDTFKLYDSYPNPFNPTTKI
                     KFDISDNSRSDVTLTVYDTNGKEVAKLLNGNLQPGTYEVEWNASDFASGTYYAKLKAH
                     NFIDTKVMMLVK"
BASE COUNT         6675 a         3117 c         3789 g         6270 t
ORIGIN      
        1 tattctgcaa cattcaacgg atttaacagc tacgtagctg ttccaaattc tttgggatta
       61 agtccgaccg gcggtattac actggaagca tgggtgaacc cgtcacagtt aggtccgggc
      121 actatgtcag tgatcggaaa aaattatcag acatcgtatt ttataggact tcaggtttca
      181 ggaagagttg tattttatcc gaaaggcgga gcgtcgctta gaagtaaagt aaccggtgta
      241 attccggtta atcaatggac tcacattgca ggtacttata atggtacttc tacagctatt
      301 tacataaacg gagttttgga tactatcact tatgtaaata ccggaccggt tacgacaaat
      361 tcagattcac tttttatcgg agctgacagg gttggtgctg ctacttcatt attcttcaga
      421 gggaaattag acaatgtcag gatctggggt gatgcaaagg gtctttcaca aattaaccac
      481 aacatgttta ctccgcttga gatcactttt cccacaggag aatacattaa tcttcgtgca
      541 agttttcagt taaactcaaa tggattaaat tatggaggat cggaattaaa tatcggacat
      601 acaaggaata tatcatttac tgacatcagc agtgaatcgg ttaactatct ggattacaac
      661 aacagtctcg tactgaacgg aaccacagac tacttcactg taagtaatca tgcaggattt
      721 aatgccacta atgcagtaac ccttgaagca tggataagaa gggacacaac cggtactcag
      781 ccaaatgacc agtatattgt aaataaaagc ggtggtacca acaggtttgg ttacgcactc
      841 tggttaaatt cttcaaccgg aattttaaag tttaaaataa attctgttgc cggtccttcg
      901 ggaattgttt cattgggagc aattcaaaca gctcagtgga ctcacgttgc tgctacatat
      961 aactcagcca caggaattgc tagactttac atcaacggaa aatattcaaa cggcgtattc
     1021 ttttctggga gtccgttgat tcaaaatgat cctgataatc tttttatagg aggtattggt
     1081 gctacagatc tggcagcata taaattcaaa ggtcagattg acggtgtcag gatctggaaa
     1141 accgaaagat ctttaaatca gatcaacgat aatatgtata ataatctgag tgacattaat
     1201 atggtgtcat ttgattttga tgaaaagtca agcgctgttc ataatggaaa tactacttcg
     1261 tataattcaa ctgtttttgc aggttcagct catatcagtt cttctcattt aaataataac
     1321 aacgaactga catcgcctgt actttcaaat gaactgggaa attttaattc atcccaatat
     1381 acagtcagta ctaaaagaac ttatattcct gatggatatg tgtcgggaat atgggattca
     1441 gtttacattc cggcaaacgg gtctattaca gatataaaat tatttacact gatctctcac
     1501 aaatatacat ctgatcttga tatcacatta acttctccat caggtaatgc aataatgcta
     1561 atcggttcaa aaggcagtta tggaaatgat attatgactt tattttctga tggtgctgga
     1621 aattatggat ccggcggagt tggggtaaat gatccgggaa ttactccgcc ttttagtccg
     1681 tctataagac ctgcaggtgc attctctatt ttcgacgggg agaatatgca gggctggtgg
     1741 aaactaaact gttctgataa atccagtgga aacattggat atgtacatgg atggggatta
     1801 aatataagtt cagtaccttt gcataaagtt ttgattctca atgcattgat acagggtttt
     1861 tatgatatga attcgaatga gatggttcag gattatgtaa aagcaaattt aagacaggta
     1921 aattctccat atagtataat tgattcctca gtcacaatat ttaatcaaaa cggtgttgga
     1981 aaattcaatt tctcaaacat aacaaacgca acggaattct atattcagac cgatcacaga
     2041 aactcagtaa atacgtggac ttccggatca aatattttta acagtgatac tttatattat
     2101 aactttactc tcgcagcagg acaggcattt ggcgcaaatc aaataaaagt agataattct
     2161 ccggtacgat atgcaatata taacggtgat gtaaataaag atgaaattat tgatctcact
     2221 gatatttctt tagtggacaa tgatgctgca aattttatga ctggttatgt attgactgat
     2281 cttaccggtg atcagtttat agacttaaat gatctgacta ttgcagataa taatgcgttt
     2341 aattttattt cagttataaa gccctgaata gaagttatgc aattaaatta taaaattaat
     2401 ttcaaacaaa acttttttat aacattttaa aaaagatttt aaaaaaataa aacaatataa
     2461 ataactatgg aaacacaaat gaaacagatt cgcgatcagc aaaaagattt atggaataaa
     2521 ttttctcccg gctggaaaaa atgggatgat ctcacaatgg aatttttaga acctatggga
     2581 attgagatcg tcagacttat agccccaaaa gaaaaagaac ttattctgga tgtggcatca
     2641 ggtaccggag agcccgggct tactattgca aatatgatca gggaaggtaa tggtaaagta
     2701 attgcaactg acatttcaaa tggtatgcta gaaatagcct gtgagaatgc taataaaaga
     2761 ggtataaata attttgaaac tataattagt gatgtaagtg atcttccatt tgaagataat
     2821 acatttgatg cgatcagctg cagattcgga ttcatgttct ttcccggaat gcttcaggct
     2881 gcaaaggaaa tggtaagagt attaaagccc gggggtagaa ttgcaacttc tgtctggggt
     2941 gctccggaga aaaatttctg gatcactact gccatgaaaa cgctgaaaaa aaatatggat
     3001 cttccggaag ttcccaagga cgctcccgga atgttccgat gtgctgagat cggacttatg
     3061 gctgaaattt ttaaacaggc aggtatgaaa aatatttctg aaaaagaagt cagcagtaaa
     3121 ttaaataccg gtactgcaga agcttactgg aattttttaa atgatgtggt agcaccagtt
     3181 gtgtcagcca tgagtcaggc gaatgacagc ataaagaata aaatcaaaga tgaagtattt
     3241 gatcttgtaa accaaaaata tccggaaggt aatgtagcaa tagacagcag tgcttatgtt
     3301 gtttacggtg aaaaataaaa tatgcaagtt ttcgcagagt ccgaagatca tgtgattctg
     3361 cgaaaacttt ttttaattta aatgagatac ctgatgatga aaatcaggga tctcctttca
     3421 catctgtcca aaacgtttag tcataatatt taagattcat tattttttac ttgctttttt
     3481 taacgacaaa aaagcaacaa aaaaagccgc cctgagtacc ggggataata actttccgga
     3541 aattctttcg tcccgagtac tcgggactgc aaaaatataa tctcctcaac cgttagaaaa
     3601 gcactaacgg tttcgtcaaa caatatattt ttcttaacgc cattgctcag aatttcttat
     3661 cggcaattta tcacaggcgg gttatattca gcaaaattta ttttaaatta aaatgtaagt
     3721 ttagtttttt aattaaaata ataaactaca tttgtaaagg tttttcaaac tgttttattc
     3781 atttttgaaa tgtttatgac gctactgatc tccttttcta tataatggtc acttatatat
     3841 tcataaatta aaactaaata ttagaagagt aagtccttac ataatgaaat taaatacatt
     3901 caaaaaagta attagattgt ttaaatgatt taatttattt taataaaatt ttttaatata
     3961 caatttacga atggctaaag cgaaacagaa aggcaagtca acggtaagtt ctctgaaaac
     4021 aaaagctcat gacacatcag aaggatttac aaattatgat aattacattt acattgctct
     4081 cataattctg atagttttat ttttcttcag agaaggtgtc ttcggaggga agatatttgc
     4141 aacaagcgat aacatcgcat ccgaaagttt caggactttt ctcgatgatg caaaaaagaa
     4201 cgggatcttt ccgctatggg tgccttacat tttcatgggc atgccgaatt ttcccatcat
     4261 ctcatataat cccagagtat atgatttctt ttacttcctg tgggacagcg gctacggact
     4321 gctgaccaac tcttcggaaa atccggtgct gcctattgta atgtattatg tagttttcgg
     4381 gattggattt tatctttacg caaaatacaa attcaaaaat aaactcatag ctctgtattg
     4441 tgctttatcc gctacatttg ctactgactt tattcaaagg atagtcgtcg gacataatac
     4501 aaaagttctg tcgctggcat tttttccatt gatacttctt gctcttgata aactcataga
     4561 ttctttcagc gataaaacga aatcactttt ttcatttcag aatctgctga acctgttttt
     4621 ccttaccatc atgcttcata tccagttgaa ttcaaatcac attcagatga tattctactg
     4681 ctatctgatg cttggtattt atgtcggcta tttaataatt tataatttag taaagaaaac
     4741 aaatgtctcc ggcatgatca aagccggtgc attttttatt gttgctgccg tcattgctct
     4801 tgcgatgaat gcagatgtgc tgatgacgat gaaggagtat aataaatatt cgatgagagg
     4861 tgaggcggga attctggcaa agctggatac gaaagttgcc gacgatcagc cgcttgatta
     4921 tgaatatgct accaactggt ctttcagtcc gggcgaagta attacttttg tgctgccgta
     4981 ttattatggt tttggagatg tgcaggtgca gggtcagaag gcaaatctct attggggaca
     5041 gatgccgttc acgaccaatc cgatgtactt cggtgtagtc acgcttgtac tgtgtctgat
     5101 cgggatcata tataatttca aacggaacgc tgctgtccag gctatgactg tcatagccgt
     5161 actgttttta tttctttcat tcggaaggaa tttctctctg atattcgata tgttttataa
     5221 tttctttccg tacttcagca gcttccgtgc acctgtaatg atccatcatt ttatgaatat
     5281 tgcaatagtt attcttgccg gattcggact taagtcaatt gtagatattg taaaagaagg
     5341 actccatctg gaaaaattta aaaaaatgtg ttacgtcttt tttggaattg cagggctgat
     5401 gtttcttatc tcaattattg gttttgaaaa ttcatataca gattccattt caaacagttc
     5461 actcattaaa aaacttcagc agcagggagc aaatgcacag cagatcagtc agtacgtcag
     5521 ccagatctca acagtcgctt atgaaaatat tgtcagtgat ctgaggctgc atgcattttt
     5581 tattgcaatt gtcatcggac ttgctctgat gtattcacag aaaagtcttt cccttaaaat
     5641 gtttcttatc ggtgtcgtac ttgtcggact tattgatctc tggaatgtaa attcaaaaac
     5701 tcttcactgg gataatccgg gagataataa cagtgctttc ggtgatactg attatacgaa
     5761 ctggctattg aaaaatgctc cggatactta tacttaccgt gtcgctgaaa tgaacggagg
     5821 cagactctcg acaaataatt ctatgtcata ttacagaata cattctttta atggatatca
     5881 cggagcaaag atcagaatct atcaggatgc ggtagatgta gccggtgata ataatccgat
     5941 gctgctttca cttggcggcg ttaaatatat tattacagat aatccgattc aggacagctc
     6001 atatacacag gtattcaaag gctcgagact tatctatgaa aataataatt tcataccatt
     6061 ggtcttcttt gctgatgaag taaaagtgga aagcggaatt aacattctca ataatatccg
     6121 tgatctgaat ttcaatccgc tgcagactgc ttatgttgaa aaagaaattc cgcagaagat
     6181 cgataagccg gattctactg taagtgctaa gcttgtaaaa ggcgaaattc atagtcttga
     6241 atatgaagta aatgccagcg gaaataattt gcttgtgttc agtgagatct atctgccatt
     6301 cggatggaaa gcttatatag acggaagtcc tgcagagttt tacaagaccg attatttcct
     6361 gagatcgctt atcgtgccgc agggaaaaca taaagttgaa ttcagatatg agcctgaagt
     6421 ttattatgcc ggaaaaaata tcagcatggc ttcaaatggt ctgctcggtg tgatactgat
     6481 agcggggatc atcggatatg ctacccgaaa gaaaaaaaat gatgacagaa aaacagaaaa
     6541 accggaaaat gtaaataatt aaaacatgat cacaactgaa aataaaaaag taaagatcat
     6601 tgcagatgtg actttactgc aagaaggcaa agttctaatg gtaaaatatt cagacaaaaa
     6661 taagtatgat catcagagcg gttggtttct tcctgatgat ctggtcagtc acggagagca
     6721 tcctgaagat gcggcttcac ggatactgaa tgagcagttg ggaataatag attctgcacc
     6781 ggtatttgat catatagaat ctttcacggg aaatgacggg tcatggcatc ttgtatttca
     6841 ttttaaatat aaagcagaaa atttacctga tgttgtaaaa tcagaagatg tagattctat
     6901 ggaatggttt tcacttaatg cattgccgcc gaaagaagaa gtcgctcatc acggctgggc
     6961 gttatatact ttagcggaga tgaacataaa ataattatct ttcgaaattt ttaaacaaaa
     7021 catccgaagc caggataact tcggatgttt ttgtttataa tccatacatt taatatctga
     7081 atatcaattt atcctctccg caaccttcag cagtttttca gtatcgatct tttgtccggt
     7141 ctctttggat tcagtattta cagttacccg taatgctttc atcccgctaa gaccctgcat
     7201 ctcactcaga tcaaattttt cgatctcttt cgtcagatat ttttcagact gtaaataatc
     7261 tatataacgc agatattctt tttcttctga agtctgtgaa taaacaacag cgatcttgcc
     7321 gggttgagta agtctctctt cagttccttt tatctcagcc ttatcgatcc tttttttcat
     7381 gatctcataa cgcacgttgt aagtaccgtc cacatcaaat ctcttttcgt cgtagcggaa
     7441 tctgatcgta agcggactat tctgaacaag aatcagctgt gctgtctgca ggggaatact
     7501 aagttcattt tcaagctctt taactttaca gataatacca catgtaagta ttagctgcca
     7561 aagccgtata tttttcagat aaagattatc aaaatgaccg tcttcattca gtgccgcgcc
     7621 gatgtaaata ctatgttcca cgccgtctgt tttgtatctc tcgaaataat gaggatatac
     7681 tttttgaatt ttttcctgtt cggaatcaag atatgtagtg atcatgctta caagcgctgt
     7741 tgaacttttt tcaaaatctt ttcttctttt ataaattatg ccgaagtcca ggtcaatttc
     7801 ttttcggaaa ttttcgatct tatctgatac ttttttatcg aatgatccga tgatgtcaaa
     7861 aagagggtaa acttctgaat ttacgaattc cacaatattg ttttcatcac cggagtttat
     7921 tgcaaagttc agtgatttga tctttttctc aattcgatag ataagatttt tgagtatata
     7981 aagaggtttg aaattaaatg attcattcaa tatatcattt atcatttcaa gattttcaat
     8041 aagatcattt ttaatggagt cgtttcttat aaccgaagaa tttctaatat cagataatgc
     8101 atatagcgga taaacattct ttacaatgat ctcttccatt tcaatttcct gtttaccgtc
     8161 agcatttaat tcattatcat tagcaagtaa ataacttaca gcagcatttt ggaatttcca
     8221 ttcaatggac ggatgaattg cagtgcattt ttcttttatt accgactgaa ttcttttatc
     8281 aaattcatct ttaaaccttg ctatggataa tccaagcagc ggataaattt cagtaagttt
     8341 taatgtgtta aatgtattta ttactgaagg ctcctgagaa gcaagctcaa gtacagctat
     8401 tactttattt tcaaatatca gaggtgccac cattagattt ctgtaaccat ttttgagaat
     8461 atgcttttcg gtcggtgtgg cttcaggata atcttcaata tcatctataa ttaaaacctt
     8521 tttttcttta acgcacattt cataaacaga tccggcacgg tctgtattta aatcgcaaaa
     8581 gcagctgagc tgaaaattat ttaacaccct gttgccatgt aagaacatta aatccttttc
     8641 gctgtctatg ggcgctattc ccatggtcac tgaactctct ctgagcaacg atctgatatt
     8701 atgctgaagc ttcagaaaac ttgtctgcat cgaaacagtg tgtttatcaa tcaggtcttt
     8761 ttttattgct gaaagaattt ctgtgtctgt aatgtttata gcgttaacta cgataaagcc
     8821 tgtatactca taatcttcag gaggaataat tttttttaag aattcaatat cgtaaattct
     8881 tctgtagatc tcttttcttt cctcttcagt gaattttctg agtttacctt ttactattac
     8941 atctgcaaat tcatttgcca tatttaattt gaaatacctg tctaacccgg tctccggatg
     9001 tggaatttta taaacgattg gaatttcaaa tttaaaatca aatccatata cataatttaa
     9061 aatatatgca taaccgccaa cgattcgtcc gaaattaaat gagacttcat caatattaag
     9121 gtctccatct aaaacctccc cgccgttcat tattatttca cggtattttg gtgaagtaaa
     9181 gaattcttcc tttttaaata ctgtagttaa ggcgtatgtt tgcttttccc agaatgcatc
     9241 cggaaaaaca aataacatca tggtggatat tagcttttcg taaggcttta catcttttat
     9301 ttcgttaaat gtttctttga ttcccggatt tttatttatt tcagtcagaa tgtttgctgc
     9361 gaattctttc ttgatatcat tagtacattc agcagcagcc tctatatgtt caatcaattt
     9421 attgaaactg atagaaacct taaacggaaa tttgtaaatc gaatttgtaa attcttccgt
     9481 agtctgattt ttaatattta aattggcaat tcttatgtct gattgagcca tggtattaga
     9541 ttttaattta atacaaactt aacctgatta atattttaaa tcaagaatta tctgataaaa
     9601 tttattataa aaatggtttg gataaatttt tatacatatg tagaagttct ttacgccata
     9661 ttaattaaat tgctgttaga ttccgcctcg ttggtgttcc aaaaagttta ccttattgac
     9721 agttgaaaat tactgcatat ctaatgcaac tttttgatca actacaacgg cacaattctc
     9781 gtgattgatt aaccgaaaaa atttaacttg attttgtttt tttttatata taaatatgtg
     9841 taaaattaaa aaaaatattc ccgccccaaa cgagaacttc taaaataaac attaaattaa
     9901 taacggtgaa aatatgaaaa aaataattat tcttgtattc ttatatttat tttcagtttt
     9961 aaattctgaa gctcagtgga tacagcaaaa ctccggcaca aaccagaatc tctatgacat
    10021 tgaattcata aatgaaaaaa caggatgggc ggtaggtgat gcgggagtag tgatcaaaac
    10081 tacaaacggt ggaactaact ggctctactt accaaaccct tctccattga tcagtccaaa
    10141 tttatggagc gtgtgtccta ttgatgagaa tattgtatat gtgactagta gcggtgactt
    10201 tattatgaaa acgacgaatg gaggattgaa ctgggatgta cttcattcat gtccttcatg
    10261 taattcttca acaaaaggaa tttatttttt aaataaagac acaggctggg tattaggagc
    10321 atacaaagta tttcgaacat atgacggagg tattacttta gattcctttt atgtaccctg
    10381 gtttacaaat tttgatattt attttaaaga tattaatacg gggatatttt gcggtactgg
    10441 aagagttttt aaatcaactg acaaaggaga aaattggatt gacactcaca tacctgttgg
    10501 tggaagtttt ccaatgttta ggaaacttgg attttcagga aacaatattt ggttaggcgg
    10561 aggaaatcct aaaatttaca aatcaactga tttgggagaa aactggataa tctccgattc
    10621 gagcattgga gctgatggaa ttggttttgt aaacgattca attggtttta taggaggagg
    10681 attaaataca ctttataaaa caacaaatgg cggatataat ttttacagac aaaaaacgga
    10741 tccttctagt cttgcattta tttcttcaat agcatttgtg aatgaactta ccgggtggta
    10801 ttgttgtgct gttggaagaa tatacaaaac aacaaatggc ggagagtggc ttacaaatat
    10861 taattcgcaa tttgaagaat tatcatctga agattttaaa ttattacaaa attatcctaa
    10921 tccatttaat ccatcaacgt acattgaata tcaattattt aaacagggat ttgttgagtt
    10981 agatgttttt aatattacag gtaaaataat ttcaactatt gtaaatgaaa acaaatctcc
    11041 gggtaattac aaagttaaat ttgaaagcag gggaattcct tcaggaattt atttttataa
    11101 tttaaaaata gatggtaagt tagtaaatgt aaagaagatg attattttaa aataattcat
    11161 aatgctaaaa taatatgtaa atgaaaaaaa taattttaat atttactata attctactct
    11221 taaaccaagg tgcagaagcg cagtggatac ttcaaagttc atttactaat aacaatttat
    11281 atgacattga gttttataac aggaataccg gctgggctgt aggtgacgga ggtacaatat
    11341 taaaaactac aaacggcgga acgaactgga ataacgtgcc aaatcctgct gtaggaaaac
    11401 cattgtcaag tattcatatt gtagattcta atgtctgcta tgtcgttggt tggtttgaaa
    11461 cgattataaa aactacagac ggaggcacga gctggatagt catcagaaat aatggctggg
    11521 gattcggatc aagttatgat gcagtatttt ttattgacga aaatacggga tggattgccg
    11581 gaaccgggca gaaaattttg agaacaacga acggctgtga ttctataatt acagattatc
    11641 tctttacagg taatcttcat gatatgtatt tcaaagatgc attaactgga attgtcacag
    11701 gttcttcatt aggaagcact ttcaaaacca caaatggagg tttgaattgg tttgaattaa
    11761 atattcagtt tggttttaca gtatctacgt tttataaatt atcatttata aatgatcaga
    11821 caggctggat tgccggaagt gataaaagag tatataagac aacagatttt ggtgatacat
    11881 gggatacaat atcaagaatt ccaattagtc agaattcaat atcaatgaga tttgcagagt
    11941 ttattgatga gaataccgga tttgtcggag gtgaacaggg atatcttttc aaatcaacta
    12001 atggaggata taattttcaa agacaaaaca cctcatcttt tccaccaaat gaaattattt
    12061 ccatgcattt atacagtgat tcaattggat ggatggtagg tggtgaagct aaaatttttt
    12121 caacaacaaa tggaggacaa attttagtaa acataaataa ctcaaattta aatgaattaa
    12181 ataaacttgc attattccaa aattatccaa atccgtttaa ctcctcaaca aaaatagaat
    12241 tctcattagc taaaaacagc ttggcagaaa ttaagattta tgatattcag ggcaaagaaa
    12301 taaaatcact tcttaaaaaa acacttactg caggaaatca caaaattaac tttgatgctt
    12361 ccggattgta cagtggtgta tatttttatt ctttaattat aaatggtgta aataccgaca
    12421 tgaagaaaat gatatatttg aaatgaaatg actgaatact gatcttaaat aattcttagt
    12481 gtcttagtgt cttggtgtct tggtggtgag taggcatgac taatttatta ataaattgta
    12541 tttgaaacct cataatggta tttttgaaaa actaatttta agcaaatgaa atcaaataca
    12601 tacagatctt tagtaataat gttcgttgtt ttattttcga catcttttta cggatgcgga
    12661 tataatacat tggttacaat ggaggaaggc gttaattcag cctggtcaca ggttgaaaat
    12721 caatatcaga gaagagcaga tctgattcca aatcttgtaa gcactgtaca gggttcagcg
    12781 gattttgaaa aaagtgtgct tactgaagta acagatgccc gcagcagggt aggtcaggtg
    12841 caactgaatg ctgacgatct gagcgaccct caaaaatttc aaatgtttca gcaggctcag
    12901 gatcagcttt cgagcgcatt atccagactg cttgtagtgg tagaaagtta tccggtgatt
    12961 aaatcgaatg aaaacttttt gcaattacag tcacagcttg aaggcacgga aaacagaatt
    13021 tctgttgaaa gaaaaagata caatgaagcg gtacaggcat acaatactga gacaagaagt
    13081 tttccgacca tgataatggc aaagattttt ggatttaaag aaaaagaata ttttaaatct
    13141 actgcgggaa gcgagaattc gcctgacgtg aattttgatt tcaagaaaaa ggaaaaataa
    13201 gcaggtaata tattttagaa atttaacatc tgaataagtg caggcagtaa taatggccgg
    13261 aggatttggt accaggctca gacctcttac taataatatc ccaaagccaa tggttccgat
    13321 tgcaaacaaa ccgatacttg aacacattat caatttattg aaaacacatt cagtaaagga
    13381 ttttgtagtg ctcctttttt ttatgcctga agtgatccgg gaagaacttg gtgacggcag
    13441 taaatatggt gtaaggatca ggtatgtagt gcctgatcag gatttcggaa cagcaggagc
    13501 tgtgaagctg gctgagcagt atattaaagg taaatttatc gttatcagcg gtgatgtgct
    13561 cactgatttt gatcttactg caatttcgga ttttcataaa aagaaaaaaa ccattgccac
    13621 tcttgcatta tacagatcaa agaatcctct gcagtttggt atagtcctga cagataagaa
    13681 tgataagata gtcaggttct tagaaaagcc ttcttcatct gaagtatttt ctgatacaat
    13741 taatacaggt atatattttt ttaataagga aatttttaaa cacattccgc ccggtgaaaa
    13801 ctttgatttt tccaaagatc tttttccttc actccttgat aaaaacattc cgctgtacgg
    13861 tcataagaca gacggttatt ggcgtgacgt aggaaatctg gaggaatata tggatgcaaa
    13921 ccttgatgta ctgagcggta aactcagtta tatcaaagct gcaggtaagg acggaaactg
    13981 tatcagtaaa acttctaaaa ttgacaaaga tgcagttata gaaaacagca taatcggaga
    14041 aaatgtaatt attgaaaaag atgtgatcat cagaaatact gtgctttgga aaaatatcag
    14101 aatatgttca gattcgagat tactttttga tgtggtagga agtaactgtt acattggaag
    14161 agggacacga ataaacgatt acgttttcat tggagaaaac tgcgttattg gcaataatgt
    14221 ttttataagt tcgagtctga aaatatggga caataaacag atagaaaata atcagaaagt
    14281 cacacgaagt cttatttacg atgataagtt ttttgcagac cttttcacgg attcgaggat
    14341 aacgggactt tcaaatctgc agataaatcc ggagtttggt gcaaagcttg gttcagtcta
    14401 tggagcattt atcgggcagg gaaagagtat tataatagca cgtgacagtg attacatttc
    14461 caatatgata aaacgcgcga tcacaagcgg acttatgtca tcaggcgtaa atgtgatcga
    14521 ctcacaggta gtgccgatcc ctatgcttag acaggaactt aagagcggtg aaggttcggg
    14581 agggatcttt gtaaggaaat ctccatttga cggaagatcc tccgatataa tattttttga
    14641 taaagacggg aaagaccttt cgggtaacaa aacgaaatcg atagaaagat tattttttag
    14701 tgaagagtat aaacgtgcag attttgaaaa agtaggctcg ataaaatttc aggagcgttc
    14761 aaacgaaaaa tatacaaagc actttctgga atgtctcgac cttcccgaaa taagaaaaaa
    14821 gaaatttaaa atagtaatag actattcata cggaattgca tctactattt ttccgaatat
    14881 actgggagag tttgactgtg atgtagtttc attatcagct catcttgatt ccaaaaaagt
    14941 aacaagaccg ctggaagaat atcacagttc acaggaacat tttacatatg tagtaaaatc
    15001 actgaaatat gattttggat ttatgataga tgccgggggt gaaaagatct ggatgtcaac
    15061 tgctgaagga aaacttctcg aaggcgacag atttcttgtg atagtattaa agatgtttct
    15121 gatggtgaca aaaaacgtta agaagatagc agttccggta caggctacgg gagagatcga
    15181 tctggttgca aaagaatttg gagtggaagt aatacgggtt aaaaatactc attattctat
    15241 gatgatggca tgtgatgatt cggaggttaa atttgtcgga ggtacgagag gcggattttt
    15301 attccctgaa tttctttatg caactgacgg tatattttct gttgcaaaga taattgagct
    15361 tatcgcaaga agcggggtat caattgatga tctgcacaga actacagcag gacttcattt
    15421 acttaaagaa aacattgaat gtgccaggga agacaaagga agtataatga gaaaatttat
    15481 ggaggataat actaaataca gacaagagct tatagacggt gtaaagatct tcattgataa
    15541 aaatactact gtcttgtgta tccctgattc tgatagaaat ctgatccacc tgaatgtaga
    15601 atcaaataaa ctttctgttg caaaaaaata tcataaagaa tataaaaaga agatagaaaa
    15661 atatttaagt aaaaaataaa aaacttaaaa cagtaagaaa aatttattat gaatataagt
    15721 gaaatattag acgaaaaagt tattagagtg gattttgaat gtaaagataa agaagatgct
    15781 ataaataaaa tgatcgaact tgcctcacat tccggattaa tgattgataa agaaaaagtc
    15841 agagagtgtg tatttcagag agaagagctt gtctcaaccg gtgtgggcaa aggttttgct
    15901 attcctcacg ggaaaacaga tgagataaaa gatatagtag ctgcttttgc agttttaaaa
    15961 gatcccatag attttgactc cattgatctc gaacctgtca gattcatttt tcttattatt
    16021 ggaaaagaaa ctttactgaa tgcacatatt aaactgctga gcagaatttc aagacttatg
    16081 aataaagatg aattcagaga cagacttgaa gaaacaaaat ccagtgaaga ggtattaaaa
    16141 ttattccgag aagaagagca aagtgtattt gacatctgat gttatttaat attcaatcac
    16201 aattttttag atcacaggta atttctatat ccgcaactct cgattcaata aaataaatct
    16261 taaatatttt gtataaagaa attttatata atatttcttt tcctgacaga caagtacatt
    16321 attgtgaagt cgggataatt ttgactgacc ccaaagagag tgaatcatta ttctacatgc
    16381 ctgtatggac accgggatca tatctgatca gagagttttc aaaaaatact gaagatataa
    16441 aagctgtttc atctgatggt gaagaattgt ttactgagaa gataaataaa aattcatgga
    16501 gagtatttaa ccctgataaa aaaaatataa atttcagtta cagagtttac tgcaacgaac
    16561 ttacagtaag gacgagcaga ataaacagtg accatgcatt cttaagtccg gcgggaattt
    16621 tcatgactgt agaaggaatg gaatccgtaa aatgtattgt gaagttagat ctgccggatg
    16681 aatggaagga aatatcaact ggtttaaaaa atatttcgga aaatatttat tacgcagata
    16741 actatgatat tctgatcgat tcaccgctgg agatcggaaa tcacaatata ctggaattcg
    16801 aaatacaggg agtaaagcat tttataagcc ttaccggttc tggaaattat gacggcgtaa
    16861 aattgattca tgattttaaa aaaatagctg aagagcagat aaattttttt ggcggggaaa
    16921 taccatatga gcattacact tttataattc accttgtaga aaatggcggt ggaggtcttg
    16981 agcatcggaa ttcttttgtc gtgcagatgg taagacttaa tttttgtgat aaaaaacttt
    17041 acaataaatt tctcggactg gtctcacatg aatttttcca tctatggaat gtaaaaagga
    17101 taagacccgc cgagctcgga ccttttaatt acaatgaaga gaattataca aaaggattgt
    17161 gggtggcgga aggctggaca agtttttatg ataatgtatt tctgagaaga tgcggactta
    17221 taaatgacga ggaatatttt gaatttctcg ggcatgagta taatgacata atgagataca
    17281 gcgggagaca tcatcagtca ttaacggatt cgagttttga tacatggata aaattttacc
    17341 gaaaggatga aaattataat aactctcaga tctcatatta cacaaaggga gcattggttg
    17401 caatgatgct ggatctggaa ataatcagaa atactaatgc tgaaagatct ctcgatgatg
    17461 tgatgagaat attatttgat gattataaaa aagataatac aaaaggtttt acacaggaaa
    17521 gagtaaaaga aatttgtgag tttgtatccg gaataaactt tgatgacttc tggaaaaaat
    17581 atgtaaatgg taaagatgaa ataccgatgg aagaatacat aagtctggca ggtcttgaag
    17641 ttgaagatat tgctgacact gcttcgagtt ttctcggtat agaatccctg acacaggagg
    17701 gtagattgat aatctcaaaa gtatttaccg gcgggtcagc atatatctcc ggacttaatt
    17761 ttaaagatga gatcatttcg atcgacagct acagagctga tgagaaaatt gcaaagaata
    17821 ttctctcggg taaaaaaccc ggtgatgtta taaaagttat gataagccgt gacggtattg
    17881 taaaagaaat aaatgtaatg cttgcaaaac catttcagct ttttaagatt aaaagaatgg
    17941 aagcgatatc atctgatcag gaaagaattt tttataaatg gataaatggc tgagtttaat
    18001 tttaaatatt aaattttaaa taaaaaataa gcttacaatt caagaactaa aattttatat
    18061 gttatgaaaa caaaatatat atttactata attatgatga tatttacggc agggtttaca
    18121 tctgcacaat atcagaatat attcatatca agtacaggtt caccaaatga gcctgcaatt
    18181 tgtatgaatc caaaaaacac agatcagata gtggcgggat caaacctgaa cttctattac
    18241 tactctacaa acggaggata taactggacg cgtgctaccc tgacatcaac attgagtgtc
    18301 tggggtgatc cttctatcgc agtagataca gccggccatt tttattatgg tcatcttaca
    18361 aatccacagg gaagttattt catagacagg atcgtagttc agaaatccac gaacggcggt
    18421 atgaactgga acaacggagc attcaccgga tatgtcccgc caaagcagca ggataaagaa
    18481 tggatatgtg tggatccgcg gaacaataat ctttatatga cctggacaca gttcgacgct
    18541 tatggaagtt catcaccttc tgacagctcg gcaattttat tttcaagatc gactaatggt
    18601 ggtgaaagct ggagtgatgc tgtcaggata aataaaattg cgggaaattg tatcgataac
    18661 ggaaatacag tcgaaggtgc agtgccgact gttggtccaa atggtgaaat ttatgttgca
    18721 tggtccggac ctttgggtat tgtaatggac agatctactg atgaaggact tacctggctc
    18781 gacgaagaaa tttttgttac aacacagccg ggtggatggg attatgctat acctggtatc
    18841 agcagagcaa atggattgcc ggttacgatc tgtgatctat caaatggtcc tgacagagga
    18901 acaatttata ttaactggtc tgaccagaga aacggtacta ctgacacgga tgtatttcta
    18961 ataaaatcta ctgacggtgg aaatacatgg ggacaggtga aaagagttaa tgacgatccg
    19021 ccgggcaagc agcagttctt tacgtggctg acaatcgatc agaaaaacgg aaatctgtat
    19081 tttgtatttt atgacagaag aaattatgca aataatcaga ccgatgtata tatgggaaga
    19141 tctactgacg gcggtgaaac tattagcaat tttaaagtaa gtgaaactcc gttcaatcca
    19201 agttcttcag tattcttcgg tgactataca aatattacag cttatgacgg aaaggtcaga
    19261 ccgatttggg caagagctga tggtacttcg atgagcattt atacagctat tgtagatttt
    19321 attacaaatg tcgaacagat aaatacgatc aaaccggata catttaaact ttatgatagt
    19381 tacccgaatc catttaatcc tactacaaag atcaaatttg atatttcaga taattcaaga
    19441 tcggatgtta ctcttacagt ttatgatacg aatggtaaag aagtagcaaa actgctgaat
    19501 ggaaatttac agccgggcac ttatgaagta gaatggaatg catccgattt tgcttcaggt
    19561 acttattatg caaagttaaa agcgcataat tttattgata ctaaagttat gatgcttgta
    19621 aaatagttag tagttaatag ttaatagtta atagttaata gttagttgtt aataaacaga
    19681 gttacgaatt taaaattata aattaccggt tttccaaaat ttacagattt ttcaaaaatg
    19741 aatttttagt ttgcctcaaa gtcattaagt tcaaagaatt ttattttact aaccgcaaac
    19801 tattaactat taactattaa ctattaacta ttaactatta actattaact a
//
LOCUS       DAORVQ010000041        19055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1179_length_19055_cov_6.139789, whole genome shotgun sequence.
ACCESSION   DAORVQ010000041 DAORVQ010000000
VERSION     DAORVQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 19055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19055
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1179_length_19055_cov_6.139789"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLX80_05180"
     CDS             <1..227
                     /locus_tag="PLX80_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase"
                     /protein_id="HQY20221.1"
                     /translation="SKKSSEIMKLIKSDSTKKFDRVYDFVIEFGGIDYTIAKAKEHSK
                     TAVDALDTFDDCEAKNSLTEFAEIISGREF"
     gene            complement(238..2022)
                     /locus_tag="PLX80_05185"
     CDS             complement(238..2022)
                     /locus_tag="PLX80_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQY20222.1"
                     /translation="MKSTLYEMFFDKASKLNPDKNIFYTKIDDEYIPKTFGEFLNDIY
                     KVTLYFRSEGISKGDRIAIISENRTEWAAVDFACIFMNVISVPVYTSISGSQMEYILK
                     DSFAKICFVSNSHLLEKIIAQKNNLPSLEKIIVFNETDTNKYSSEPVKNFSEIIDANV
                     NTSTKEIIDFLRSVSAHIKLHDLVTIIYTSGTTGIPKGVMLSNKNLSSNIQACMEVLT
                     INESDRFLSYLPYSHGYERVAGYYLAFFSGAEIYFAQNIDTLSIQLKEVKPTIVITVP
                     RLLDKIYNRLMKSPESMKSDFQKKIFLWAVEIAKDKSISKKSIKWKIADRLVYKKIRD
                     KTGNYIRFFVSGGGALNKNIGEFFDRIGLMVLEGYGLTETSPVIAVNHPDDNRYGTVG
                     KILNGVQLRFTDENEITVKGDLVMEGYYKDENSTAETIKDGWLYTGDIGELDSEGFLK
                     ITDRKKSLFKSSGGKYISPTQIEEIVSHLDFIENILVIGNERMYVSALIVPDKNELIS
                     FAKKNEISNNKYSELLKDTKLIKLIQKEIDTVQKDLANYERIRKITLLEKPFTIESGE
                     LTPTMKVKRNVVGKLYEKEIEEMYKNAQ"
     gene            2282..3001
                     /locus_tag="PLX80_05190"
     CDS             2282..3001
                     /locus_tag="PLX80_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20223.1"
                     /translation="MNAQTSKTYYTYSKPSWTVGFGPAWNLATNDAYGRANYSTNAQV
                     FKDNYGMRWGWGGYIFGKYSPGKTKADRIFLGFDYKGMTNTDFESGANKTSFDIITFD
                     AGYEYVFRPGSNSRFWSYYGLGLTGNLISGEFLPQTATATSIKRNLESTFRVGMEIKA
                     GLEFVLNNSKRNLGINIGGRYNLTNLFNADNALPTAGQTDNFNLNDGGEAGGPGFKRY
                     IGIFSLDVGLNIYPDVKKTKR"
     gene            3319..3897
                     /locus_tag="PLX80_05195"
     CDS             3319..3897
                     /locus_tag="PLX80_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20224.1"
                     /translation="MNCSVKDPSGNIIATIAMSDNGVLPDTTAGDGKYSAAVNVNNIS
                     CLLVGNYAIDYLALNTSGLYSNQIVSDIIVVNSANMPPVITGTNLPDSVVRPLPGDSL
                     LLTISVNVNDPDGICDLKDVTFVTVRPNGVTFPPIPMSDNGNGEFLFSNYVSYSTDPT
                     SYGYFKYTFTAKDRSNIESIPVTDSIKFVLPQ"
     gene            3894..5519
                     /locus_tag="PLX80_05200"
     CDS             3894..5519
                     /locus_tag="PLX80_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20225.1"
                     /translation="MKTIILILLLFVNISFAQNIHKAEDFELIPFGKDFSSQLRSNPL
                     FLNQIVVPNNSTTPISNFITDILLNGDTVWFGTGSGLMRTIDNFKSFQNYYGLAPFGT
                     DDIAGFNLNRNVLAASTAVSQEINGESVPTGTGIKVSTDYGLNWSAYPQPTDSQSDST
                     IVYGSNTIYALPVTVTQQNLTYDIAITKTQNDPSNYTIWICSFAGGLRKSTDYGATWI
                     RVLLPPDDLDSINVNDTGYTFTLNPRDNLNQRVFTITAVDDSTLYVGTAGGINVSNSW
                     GVNWRNYKFQNTNPNANNNGISGNFVVSLDVQKYDGNTIVWGATLKAEFANEFDALSY
                     TSNGGANWNNTLSGNKPNGISFYRNIVYGYTNSGLWRSEFGNFNWAKAGVIYDERTKD
                     QLRTSQFYAGNHIGDTLFIGSADGLLQTQELGQPWIGQWTIYRSVTNIDLSSELLTYA
                     APNPFSPDDEVTRIYYKSPSTTSKVTIKVFDFGMNPVRTIIQNATRTGTEVLYTSWNG
                     RNDNNQRIANGVYFYRVEINDDDPVWGKVILLE"
     gene            5544..6566
                     /locus_tag="PLX80_05205"
     CDS             5544..6566
                     /locus_tag="PLX80_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20226.1"
                     /translation="MKKYILILVFTAIGFQHADAQLGSYAGAFSRLGFGARGMAMGNA
                     VSSDIFGDISGIYNPALATFQNDGKVDLGYTFLSFDRKLNFVGYTQKFKLPNQKEGGA
                     GITVAWINAGVNDIDGRDNDTRQIGMFSTFENEFYLGTSFILSDQLSLGIGFKLYYAK
                     LFDDITTSSFAIDFGGIYKAGNNLVFGLTIRDLGAKYEWQTTSIYGANGNSTEDKFPK
                     QVELSGTYLLPKDFGIVSLGFVQYLNPKPETNLDETSGSSSDNTVLRFGIETNIIKQV
                     KLRAGLDRIDFSTSDFSGNLEPTFGFGLDKNFSKTINLGVDYSVQLEPFSHDLIQNIG
                     LVFKFK"
     gene            6569..7480
                     /locus_tag="PLX80_05210"
     CDS             6569..7480
                     /locus_tag="PLX80_05210"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQY20227.1"
                     /translation="MKYLKLIISVFIITPCFFSDSYSQNDGTANTGLSFLKTGISARA
                     LAMGEAYSSVTDDASAYFYNPARLQFGEKNNILLMHNSLGQDQLSEYIAVKFPLSEKF
                     FMGVGFYTTSVSDIEVRNIPGAVLETFDSRNLSTGVSFSYSVNKEISIGLTTKLIYEK
                     IYVDEASGVGFDIGTNYTKNNLNFAFVISNLGSVNELQTQSTKLPSSFRIGGSYKFKQ
                     KDFDFDLALEGFKIMDGGLFHILTGGEAGYKDMVFLRLGYQSNYENKGLTTGLGFKYK
                     ALNVDYAFIPSVDGFGTGNSFSLGINF"
     gene            7500..9179
                     /locus_tag="PLX80_05215"
     CDS             7500..9179
                     /locus_tag="PLX80_05215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY20228.1"
                     /translation="MSSLYFKLNKFLENGGNLYKVFFVLSILLIIFKLHSLLTTDIQP
                     WDEGMYATRLLSIHVNGDFFDQYSHSVGKFYSASHPPLLIWAGYFATLIFGVNAVVLK
                     SLIFILSLLSILLILLIGKELFSLKIAFYSAIIFCSNIIFSVFSQRFQFDISYTFLIL
                     LSFYFLLLFNKTEKYKYIIYLGITFGACLMVKILVGFYIPIVIGISYYFIKDKTNFKI
                     KDLFVLTLIGILIAMPWHIYMLSTYGGEFTDYFFKFHVFDRAFIGVENSVKPSGIFYH
                     VNYMLTIIPYSILIVFSFVKDLIDRKNLDWKKIFIWIWFLSGMLILALFKTKLEVYIL
                     LILPPGCYIISLFINSIETEKFYIKALSVTFVLLNFLWFATEDIRPFLKDYFLRQDLL
                     MTGLIFILLLIFLYYSGRFIARRFDLKNTYCIFVLIFFLSINIFYLFKIPEWENKFQL
                     TEIKETIEKSGKKNIVYVATNFRHNPQFSFYFNGLDLNWDNPEYKFELIDNKDGLGMV
                     KDKLSSLKRNEYMIIVEKDYINRADYPDSQLFVPGDHKLLLKRNGYELYEN"
     gene            9183..10166
                     /locus_tag="PLX80_05220"
     CDS             9183..10166
                     /locus_tag="PLX80_05220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY20229.1"
                     /translation="MIETIRVNNIENNNEWSEFLRNQYNLFFDHRFNSYNDVFKKNIK
                     WHHLMFRIKGTNRVVAIMTGCEKIKEDGSKTFVSCDGVSYGGFLWKLKSDLITYFDVL
                     KSFKVYMAENEFRECVIRNPPFRYNNIPNEETDYALIKEGFKVSNISLTNIIDINEFD
                     FKKISGPKKRSIKKSSSVLEFDILNDVSDENKLKEFYDILQKNRELKNVKPTHSLKEL
                     FYLKNKLSDELIFFTAKIDSEIAAVCVLFRINSDMILNFYLAGDEKFKMNRVSEYLLY
                     KSIEWSKENGCKFYDIGTSDMDGKLLEGLYAFKKKFLAHGFLRKTYELKIK"
     gene            10172..11134
                     /locus_tag="PLX80_05225"
     CDS             10172..11134
                     /locus_tag="PLX80_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY20230.1"
                     /translation="MNIKNKKILLTGGAGFIGSTLVSKLVKENEITILDNFSRDSIKY
                     KNITSKNLTVIEEDVLNFEALKKTCENFKPQIVIHLAAVAGIDTVIIDPVKTLDVNIN
                     GTFNLMKALEKYSNRLERILDFSTSEVLGAYAYKSTEKSHTNFAPVGEARWTYSISKV
                     AGEHIVHSFFKKFGYPVVTIRPFNIYGPGQVGEGAIQIFIKNVVNNKDIEIHGDGDQI
                     RSWCYIDDMVNGIELCITNKKAIGEVFNIGNPKGTITISSLAEKIVSICKSKSKIIYT
                     PKNYVDVELRIPSIEKAKDLLGFNPVVDLNEGIKRTFNWYKKNI"
     gene            11134..12042
                     /locus_tag="PLX80_05230"
     CDS             11134..12042
                     /locus_tag="PLX80_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HQY20231.1"
                     /translation="MNYFYLAHSVMSLDCLKDLVSKNYSPALVIIHKSRDRKKLSESF
                     YSPMIEFCRSKKIELIETEDINNLKKRIENFKTGICVGFMDILNKDFFEIPENGILNL
                     HSGKLPEYRGRAPISRSIMNGEKYVTVSVHKIDEGVDSGDICKELKIEIAEEDDVNTI
                     YKKCSESASVILVDCLNELKGGKLEFHKQESDEKANKIINNGERKINWENNAVINYNK
                     IRALTAPYPCAYSIFKGSEYLFLKAKTSDEFNDKNLQSGTVVKVSDNEIVIKSGNGFL
                     RIFKVTDENLNEVNFKELFNEGDIFE"
     gene            12039..12767
                     /locus_tag="PLX80_05235"
     CDS             12039..12767
                     /locus_tag="PLX80_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY20232.1"
                     /translation="MKIIDAHIHIGKWSDIFFNYTSTIEQAVDVMKKSGVEAALCMPA
                     DSTSNDELFNMIKDSDFKFYYNAWIDPSDKELDNFLDKNLNEISAFKIHPSIQRLKIT
                     DLSFKKYLQMASDAGKPVIVHCGRWQEMAGFKIALEAAVKFPELKLILAHMGGDQPSL
                     YLECAEVINDSKYKNVYLGTESVREFYFMDLAVKKAGAEKVIFGSDYNLGLPLTYIPI
                     VESLNISHEEKEMIFNGNILKLLS"
     gene            complement(12785..13126)
                     /locus_tag="PLX80_05240"
     CDS             complement(12785..13126)
                     /locus_tag="PLX80_05240"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HQY20233.1"
                     /translation="MELIYHITTIDHWDKHKKNKTYKTLSLEDEGFIHCSPKDKVRSS
                     TLKFFSKEKIVLLLFIDQSKVLSEIIWEDSENNNYLFPHIYGELNIDAVTEIIEIISD
                     GDGLFIFPEGF"
     gene            complement(13143..13727)
                     /locus_tag="PLX80_05245"
     CDS             complement(13143..13727)
                     /locus_tag="PLX80_05245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20234.1"
                     /translation="MKKKYDWDVYYEHHKERPVKESLYRTLDLFKKDKIYSGHAFDLG
                     CGNGHETRELLRKGWSVTATDKNKKVKIMLDDLKTKHKNNLDIQAVSFEKITWKKTTF
                     IYANYSLPFCPANHFDKVWINIVDSLRRNGRFSGQFFGDRDEWDLVRHTKDQALERLE
                     GMEIELFEEIEKDDKTTTGEMKHWHVFDIIAKKL"
     gene            complement(13737..14552)
                     /locus_tag="PLX80_05250"
     CDS             complement(13737..14552)
                     /locus_tag="PLX80_05250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20235.1"
                     /translation="MSGKNTDYILGINQTELERLEFQHGVWKEVTDKFLEKISIQKGW
                     KCLDVGAGPGFVSQQLIELTGGAGEVTALEPGEFFLEHFRNVCERNNFSNAKFINSDI
                     ENADLEENYYNLIYMRWVIDFVPDAGNILLKLFRSLKKGGVIAIQDYAYEGIALFPKG
                     GPFDNIAEYVKSYWVAGGGDPYFTVKVPKYFRENNISLNEYSPVNLAGGCDSDIFKWA
                     YIFFKGHLKSLTELNIITSTLEKELWNDLKSHAENPDMIFFSPVIVNIIGQKN"
     gene            15301..16665
                     /locus_tag="PLX80_05255"
     CDS             15301..16665
                     /locus_tag="PLX80_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20236.1"
                     /translation="MKNSTPLNFSFKSINVFVKFCMILSLTFFSMKTDQIKADVVAVN
                     PGSPTIEVFNEATWASISSAPLTMGGFTITGAQAVTWNSDNLLFYAIVTVTPTARHLV
                     TVDPVTGICTNVGVLASTAFSTLTYNSTSGILYTMGGSGGGPVAERLYSVDINTAATT
                     FLAGPYTVGGFGEVIAYNYTDNMIYHWSGTGMEMIDPVTFIATPVVRSGAPVDEVVGA
                     VYVGGGIFQITDLVFTVSTSAFTMTSAGVATLIGPTSYRARGLGYVDPLLPVELSSFS
                     SSISGSDVTLNWSTSSETNNSGFDIERSSVSGTWSKVGNVTGNGTSITTNNYSYTDKN
                     LTSGNYSYRLKQIDFNGNFEYFNLSNEVIVGIPVKYDLSQNYPNPFNPSTKINYTLPT
                     EGLVSIKIFDMSGKEVMSLVNEVKTAGYYSVNFNASNLSSGIYFYSLSAADFTDTKKM
                     MLVK"
     gene            complement(16865..17182)
                     /locus_tag="PLX80_05260"
     CDS             complement(16865..17182)
                     /locus_tag="PLX80_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HQY20237.1"
                     /translation="MSWIYLLIAGIFEIGWPLGMKLSQTMSSKILWIILAVISMAISG
                     LFLWLAQRDIPIGTAYAVWTGIGAVGTLIVGIIYFGDSASIWRLLSASLIVIGIVGLK
                     IAN"
     gene            complement(17310..17870)
                     /locus_tag="PLX80_05265"
     CDS             complement(17310..17870)
                     /locus_tag="PLX80_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY20238.1"
                     /translation="MNTISKFRFELIDEFKLDLNTKIQISALLKISFPEEEFHGRTYF
                     KQLPHYRLLMWKDDILIGQLGLDYRVMTLNKDPVRVMGIIDLMILPEFQHQGNGTHLL
                     KELENIIDKHIHNIDFLFLVADYHKFYERCSYRLTKQKVKWLTIEEHINYGIQEKEFD
                     NCLMIKQLGDKEWKDDGELDMMGYWY"
     gene            18468..18926
                     /locus_tag="PLX80_05270"
     CDS             18468..18926
                     /locus_tag="PLX80_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HQY20239.1"
                     /translation="MNNEKAIEVLNSLVEINNDRIEGYKSASEETDSNDLKGLFMQFK
                     NTSERCITELTKEIIKCGGIPEEGTKTSGKLYRAYMDLKAALTGNDQQKILDSCEYGE
                     DKAVETYKDVFNNNSENLTAEQISLVRSQYDLIKNDHDTVKSMRDSEVTL"
BASE COUNT         6479 a         2954 c         3200 g         6422 t
ORIGIN      
        1 cttcaaaaaa gtccagtgaa attatgaaat taattaaaag tgattcgaca aaaaaatttg
       61 acagagtata tgattttgta attgaatttg gaggaataga ttatacaatt gcaaaagcaa
      121 aagaacattc aaaaactgct gttgatgctc ttgatacttt tgatgactgt gaagcaaaaa
      181 attctctgac tgaatttgct gaaataatca gtggcaggga attctaaata gcaaatttta
      241 ctgagcgttt ttatacattt cttcaatttc cttttcatat aattttccga caacattcct
      301 ttttactttc atagtaggtg taagctcgcc actttcgatg gtgaagggtt tttccaataa
      361 agtgattttt ctgatacgtt cgtaatttgc cagatctttc tgtactgtat caatttcttt
      421 ttgaattaat tttatcagtt ttgtatcctt taaaagttca gaatatttat tattagaaat
      481 ttcgttcttt tttgcaaagg aaatcagttc atttttatca ggtacgatca gtgctgatac
      541 atacattctt tcatttccaa tgactaaaat attttcgatg aaatccagat gcgatacaat
      601 ttcttcaatc tgtgtaggag aaatatattt ccctcccgag gatttgaaca gtgatttttt
      661 tctgtcagtg atctttagaa aaccttcgct gtcaagctct ccaatatcac cggtataaag
      721 ccagccgtct tttattgttt ctgctgtaga gttttcatcc ttataataac cttccattac
      781 aagatctcct ttgactgtaa tttcattttc atctgtaaaa cgtaattgca caccgttcaa
      841 aattttacca acagtgccgt atcgattgtc atcaggatga tttacagcga tgacgggtga
      901 agtctctgta agtccatatc cttctaaaac catcagaccg atccggtcaa agaattcgcc
      961 aatattttta ttcaaagctc caccgccgga tacaaaaaat ctaatataat taccggtttt
     1021 atctctgatc tttttgtaaa ctaatctgtc agcgattttc catttaatac tttttttaga
     1081 aatggattta tcttttgcaa tttctactgc ccagagaaat atctttttct gaaaatcact
     1141 tttcattgac tccggacttt tcataagcct gttatagatc ttatcaagaa ggcgcggtac
     1201 tgtaattaca attgtcggct ttacttcttt gagctgaata gaaagcgtat caatattctg
     1261 tgcaaaataa atttccgctc ccgaaaaaaa agcaagatag taaccggcta ctctttcata
     1321 gccgtgtgaa tatggaaggt aagaaagaaa tctgtctgat tcattaatcg tcaatacttc
     1381 catgcatgcc tgaatattac tgcttagatt tttatttgaa agcataactc cttttggaat
     1441 tccagttgta cctgaagtgt aaattattgt aacgagatca tgaagtttta tatgagcaga
     1501 aacagatctt agaaaatcaa ttatttcttt tgtagaagta tttacatttg catcaataat
     1561 ttcggaaaaa ttttttacag gttcagaaga atatttattt gtatctgttt cattaaagac
     1621 aattattttt tctaaagagg gcaggttatt tttctgagca attatttttt ccagcaaatg
     1681 agaatttgaa acaaaacata tctttgcgaa actgtctttt aaaatatact ccatttgact
     1741 gcctgatatt gaagtataga ccggtacaga aatgacattc atgaatatac atgcaaaatc
     1801 tacagcagcc cattctgtac ggttttctga aataattgca atcctgtctc ctttagaaat
     1861 tccttctgat ctgaagtata aagttacttt gtaaatatca tttaaaaact ctccaaaggt
     1921 ttttgggata tattcatcat ctatttttgt atagaaaatg tttttatcag gatttagttt
     1981 gctagcctta tcaaaaaaca tctcataaag agtacttttc atttatttat tctatgtgat
     2041 ttttctgaga aaattccact ttttattttt ttatttttat ctgtgacgta atgaccattg
     2101 aatatttaaa tataatacaa aaacaaagaa accgagttaa tatatacttg attgttttaa
     2161 atcaaaatac taatttgaat ctaaaatatt ccgaaataaa attatattta cgaaaggaaa
     2221 atccatgaaa agaaccatac tttttttact cattatagta tcgttattca tcttaaatga
     2281 tttgaacgca caaacaagta aaacttatta tacttattcc aaaccttcct ggacggttgg
     2341 ttttggtcct gcctggaatc ttgcaacaaa tgatgcctac ggcagagcaa attacagtac
     2401 caatgctcag gtatttaagg acaattacgg aatgagatgg ggatggggcg gatatatttt
     2461 tgggaaatat tctcccggta aaactaaagc agacagaata ttccttggtt ttgattataa
     2521 aggaatgacc aatacagatt ttgaatccgg tgcaaataaa acaagttttg atattattac
     2581 ttttgatgct ggttatgaat atgtttttcg tccaggcagt aacagcagat tctggagtta
     2641 ttatggtttg ggtttaacag gtaaccttat ttcaggtgag tttcttccac aaactgcaac
     2701 agcaacaagt ataaaaagga atttagagtc aactttcaga gtcggtatgg aaattaaagc
     2761 aggtctggaa tttgttctga acaactcaaa aagaaatctt ggtattaata ttggcggcag
     2821 atataatctt acaaacctat ttaatgcaga taatgcattg ccgacagcag gtcagacaga
     2881 taattttaat cttaatgacg gcggagaagc cggcggtcca ggatttaaaa gatatatcgg
     2941 aatttttagt ttggatgtag ggttgaacat ttaccctgac gtaaaaaaaa ccaaaagata
     3001 aatttaaatt attattaatc aaccaaatct caaacacata aagatatttt acttattagg
     3061 ttaatttaat tttattccat taagatcatc aaaaataata agtatatatt cgcagtaata
     3121 ttaatcagta ttactgcgat ttttttctat tcatgcgaaa gcgatgataa cagcgttata
     3181 gacccatcta tttccgctcc tttattatca aatgtttata tttcaaaaga ttcagttttt
     3241 acaacttcag taaatccgga tatttcattt ttcacatcta tacaggtaaa tcaaaatgga
     3301 ggtcctgcac ttgattatat gaactgtagt gtcaaagatc catcaggaaa tataattgca
     3361 acaattgcaa tgtcagataa tggagtgtta cctgacacaa ctgccggtga tggtaaatac
     3421 agtgctgctg tgaatgtcaa taatatcagt tgtctgttag ttggaaatta cgcaatagat
     3481 tatcttgctt taaatacttc cgggctttac agtaatcaga tcgtttcaga tataattgtt
     3541 gtaaattcag caaacatgcc gcccgtaatt accggtacaa atctaccgga tagtgtagtc
     3601 aggcctttgc ccggagattc tcttttactt acaatatctg taaacgtaaa tgaccctgac
     3661 gggatttgcg atctgaaaga tgttactttt gtaactgtca gacctaacgg agttactttc
     3721 ccaccaattc caatgtcaga taatggtaat ggtgaattcc ttttttcaaa ctatgtttcc
     3781 tacagcaccg acccgacatc ttacgggtat tttaaatata catttacagc aaaggacaga
     3841 tctaatatag aaagcattcc tgtcacagac agtattaaat ttgtattgcc acaatgaaga
     3901 caattatttt aatattattg ttatttgtaa atatttcttt cgcacagaat atacacaaag
     3961 ctgaagattt tgaactaatt ccgtttggaa aagatttttc atcacagtta agaagtaatc
     4021 ctctatttct taatcagata gttgtaccaa ataattctac tactccaatt agtaatttta
     4081 taacagatat tttattaaat ggtgatactg tctggtttgg aacaggcagc ggtttaatga
     4141 gaactatcga taacttcaaa agctttcaga attattacgg actggctccg tttggaacag
     4201 acgatattgc aggttttaat cttaacagaa atgtgctcgc agcatccact gccgtgagtc
     4261 aggagatcaa cggtgagtcc gttccgacag gtacaggaat taaagtctcc acagattacg
     4321 gacttaactg gtctgcatat ccgcagccta ctgattcaca aagcgattcg acgattgttt
     4381 atggctcaaa tacaatttat gctttaccgg tgacagtaac tcaacaaaat cttacttacg
     4441 atattgccat cacaaaaact caaaacgacc cttcgaatta tacgatctgg atttgttcat
     4501 ttgcaggcgg acttagaaaa agtacagatt acggagctac atggatcaga gtacttctgc
     4561 ctcctgatga tcttgacagc attaatgtaa atgatacagg atatactttt actttaaatc
     4621 caagagataa tttaaatcag agagtattta ctattacagc agttgatgat tctactttat
     4681 atgtaggtac cgcaggcgga ataaatgtgt caaatagctg gggagtgaac tggagaaact
     4741 ataaatttca aaatacaaac cctaatgcaa acaataacgg aatttcagga aattttgtag
     4801 tgagtctaga tgtacagaaa tatgacggta atacaatagt ctggggagct acacttaagg
     4861 cggaatttgc aaatgaattt gatgcactta gttatactag caacggaggt gcaaactgga
     4921 ataatacttt atccggaaat aaaccaaacg gaatatcctt ttacagaaat attgtttacg
     4981 gatatacgaa tagcggatta tggagaagtg aattcggaaa tttcaactgg gcaaaggccg
     5041 gtgtcattta tgacgaaaga actaaagacc agttgaggac ttcacagttt tatgctggta
     5101 atcatattgg tgatacactt tttatcggaa gtgcagacgg attactacag acacaggagc
     5161 ttggacagcc gtggatcggg cagtggacaa tttacagatc agttacaaac attgatctgt
     5221 cttcagaact gctgacttat gcagctccaa atcctttttc accggatgat gaagtaacaa
     5281 gaatttatta caaaagtcct tcaaccactt caaaagtcac tattaaagtt tttgatttcg
     5341 gaatgaatcc tgtaaggaca ataattcaga atgcaacaag aacaggaacg gaagttcttt
     5401 atacatcatg gaatggaaga aatgataata atcagagaat tgcaaacggt gtatattttt
     5461 atagagtaga gataaatgat gatgatcctg tatggggaaa agtaatttta cttgaataaa
     5521 aaacagagta gaaaatattt tatttgaaaa aatatatttt aatattggtt tttacggcaa
     5581 ttggatttca gcacgctgac gcgcagcttg gaagttatgc cggtgctttt tccagattag
     5641 gatttggagc acggggtatg gctatgggaa atgcagtttc ttcggatata ttcggagata
     5701 taagcggtat ctacaatccg gcactggcta cttttcagaa tgacggtaaa gttgatcttg
     5761 gttatacttt tcttagcttt gacaggaagc tgaactttgt cggttacaca cagaaattta
     5821 aattgccaaa tcaaaaagag ggtggagcgg gaattactgt tgcatggata aatgcaggtg
     5881 tgaatgatat tgacggaaga gataatgata cgagacagat aggaatgttc tcaacttttg
     5941 aaaatgaatt ctatttaggt acttcattta ttttatcaga tcagttatca cttggtattg
     6001 gatttaaatt atactatgca aaactttttg atgatattac cacatcttca tttgcaatag
     6061 attttggagg tatttataaa gcgggtaata atctggtatt tggtctgaca atcagagatc
     6121 ttggtgcaaa atatgaatgg cagacgactt ctatttatgg agctaacgga aattcaaccg
     6181 aggataaatt tcctaaacaa gtagagctaa gcggaaccta tcttcttcca aaagatttcg
     6241 gaatcgtttc tcttgggttt gttcaatatc ttaatcctaa acccgaaaca aatttagatg
     6301 aaacttcagg ttccagttca gataataccg ttttacgatt tggaattgaa acaaacatta
     6361 taaaacaagt taagcttaga gccggtcttg acagaataga tttttccact tctgattttt
     6421 caggaaatct ggaaccaaca ttcggttttg ggcttgataa aaatttttca aaaaccatta
     6481 atctcggtgt agattattca gttcagcttg agccattttc tcacgacctg atacaaaaca
     6541 tcggactagt ttttaaattt aagtaaacat gaaatatcta aaattaataa tatcagtttt
     6601 cataatcaca ccgtgttttt tttctgattc atattctcaa aatgacggta cagcaaatac
     6661 cggtttgtca tttttgaaaa caggtatcag cgcaagagct cttgctatgg gtgaagcata
     6721 ttcttcagta actgatgacg catcggctta tttttataat ccggcacggc tgcagtttgg
     6781 tgaaaagaat aatattcttc tgatgcataa ttctttagga caggaccagc tttcagaata
     6841 tattgcagtt aaatttccat taagtgaaaa gttctttatg ggagttggat tttatactac
     6901 cagtgtaagt gatattgaag tcaggaatat acccggagct gtacttgaaa catttgattc
     6961 gagaaatctt tccactggag tttcattcag ttattctgtg aataaagaaa tttcgatcgg
     7021 acttactact aaactaatct atgaaaaaat ttatgtggat gaagcatcag gagttggttt
     7081 tgatattggt actaactata ctaaaaacaa tctgaacttt gcctttgtaa tttcaaatct
     7141 cggatctgta aatgaacttc agacacaaag cactaagctg ccttcatcat ttcgtatcgg
     7201 aggaagttac aaatttaagc aaaaggattt tgactttgat cttgctctcg aaggatttaa
     7261 aattatggat ggtggtttgt ttcatatcct tacaggcgga gaagcaggtt acaaggacat
     7321 ggtcttttta agattgggct atcagtctaa ttatgaaaat aaaggtctta caacaggttt
     7381 aggctttaaa tacaaagctt tgaatgttga ttatgctttt attccgtccg ttgatggatt
     7441 cggtactgga aattcttttt cactggggat aaatttctga tcttctctaa agtgaattca
     7501 tgtcttcttt atatttcaaa ttaaataagt ttttagaaaa cgggggtaat ctttacaaag
     7561 tttttttcgt cctctccatt cttttaataa tcttcaaatt acattcactt ttaactaccg
     7621 atattcagcc gtgggatgaa ggcatgtacg caacccggct gctgtcaatt catgtcaacg
     7681 gcgatttctt tgatcagtat tctcattcag ttggtaaatt ttattccgct tcacacccgc
     7741 ctctgctcat ctgggcagga tattttgcta ctcttatttt cggtgtaaat gctgtagtac
     7801 tgaaatcact gatcttcatt ctttctcttc tgagtattct tctgattctt ttgataggaa
     7861 aagaattgtt ctctctaaag attgcttttt attcagcaat aatattctgc agcaatatta
     7921 tattttctgt attctcccaa aggtttcagt ttgatatttc atatactttt ttaattctgc
     7981 tgtcatttta ttttctgctt ctttttaata aaactgaaaa gtataaatat ataatctatc
     8041 tgggaattac tttcggtgca tgtctgatgg taaagatact ggttggattt tatattccga
     8101 ttgtaattgg tatatcatat tattttatca aagataaaac aaattttaaa attaaagatc
     8161 tatttgtcct cactctgatt gggattttga ttgcgatgcc ctggcatatt tatatgcttt
     8221 caacatacgg cggtgaattc acagactatt tttttaaatt tcatgttttt gacagagcat
     8281 ttataggtgt tgagaacagc gttaaacctt cagggatatt ctatcatgtg aattatatgt
     8341 tgactataat tccatacagc atcctgatcg tatttagttt tgtaaaggat ctgattgaca
     8401 gaaaaaatct tgactggaaa aaaatattta tatggatctg gtttctttcc ggaatgctta
     8461 tacttgcttt atttaaaact aaactcgaag tatatatcct gctgatactt cctccgggct
     8521 gttatattat ttcgcttttt ataaattcta ttgagacaga aaagttttat ataaaagctt
     8581 tgtctgtaac atttgtactt ttaaattttt tatggtttgc gacagaagat atcagaccat
     8641 ttttgaaaga ttacttttta cggcaggact tgcttatgac tggcttgatt tttattctct
     8701 tacttatttt tttatattac tcaggaagat tcatcgcacg tagatttgat cttaaaaata
     8761 cgtattgtat ttttgtactt atattctttt tatctataaa tatattttat ttattcaaga
     8821 tacctgagtg ggaaaataaa tttcagttaa cagagataaa ggaaacaatt gaaaaaagcg
     8881 gtaaaaagaa tattgtgtat gttgcaacaa atttcagaca taacccgcag ttcagttttt
     8941 actttaacgg tctggatttg aactgggata atcctgagta taaatttgag cttattgata
     9001 ataaagacgg attgggaatg gtcaaagata aattaagttc tttaaaaaga aatgagtata
     9061 tgatcatagt agaaaaagat tatattaaca gagcagatta tccggactca cagctttttg
     9121 tacccggtga tcataaatta ttgttaaaaa gaaacggata tgaactttat gaaaactgat
     9181 caatgataga aaccattagg gtaaataaca ttgaaaacaa taatgaatgg tctgagtttc
     9241 taaggaatca gtacaatctt ttttttgatc acagatttaa ttcatacaat gatgttttta
     9301 agaaaaatat taaatggcat cacctgatgt ttagaattaa aggaacaaac agggttgtgg
     9361 cgataatgac aggttgtgaa aaaattaaag aagatggaag taaaactttt gtgtcttgtg
     9421 acggcgtaag ttatggtgga tttctctgga aattaaaatc agatctgatc acttattttg
     9481 atgtattaaa aagttttaaa gtgtatatgg ctgaaaatga atttagagaa tgcgtaataa
     9541 gaaacccgcc gtttagatat aataacattc cgaatgaaga aactgattat gcattgataa
     9601 aagaaggatt caaagtatct aatatttcac ttactaatat tattgatata aacgaatttg
     9661 attttaagaa aatatccggt cccaaaaaaa gatcaataaa aaaatcatct tctgtgctgg
     9721 agtttgatat tttaaacgat gtatctgacg aaaataagct gaaagaattt tatgatatac
     9781 tccagaagaa cagggagctt aaaaatgtta agccgactca ttcgttaaaa gaattattct
     9841 atcttaaaaa taaattatca gatgaactca ttttttttac agcaaaaatt gattcagaaa
     9901 tagctgctgt gtgtgtgctt ttcagaataa acagtgacat gatcctgaat ttttatctgg
     9961 cgggagatga gaagttcaag atgaacaggg tatctgaata ccttttgtat aaatcaatag
    10021 aatggtcaaa agaaaacgga tgtaaatttt atgatattgg aacttccgat atggacggaa
    10081 aacttctcga aggactttat gcatttaaga aaaaatttct ggcgcacggc tttttaagaa
    10141 aaacctatga attaaaaatc aaataactaa attgaatata aaaaataaaa aaattctgtt
    10201 gacaggcgga gccggattta ttggcagcac acttgtgtca aaacttgtaa aggaaaatga
    10261 aataactatc cttgataatt tttcaaggga ttcaatcaaa tacaaaaaca taacttcaaa
    10321 aaatttaaca gtaatagaag aagacgtatt gaattttgaa gctttaaaaa agacctgtga
    10381 aaatttcaaa cctcagattg tcatacatct tgctgctgtc gcagggattg atactgtgat
    10441 cattgatcct gtgaaaacac tcgatgtaaa tattaacggt acttttaatc tgatgaaagc
    10501 attggagaaa tattcgaacc ggttggaaag aattcttgac ttttctacaa gtgaagtgct
    10561 tggagcctat gcatataaat ccaccgagaa atcccataca aactttgcac ctgtcggaga
    10621 agcccgttgg acatactcta tcagtaaagt agcaggtgaa catattgtcc acagcttttt
    10681 taaaaaattc ggttatccgg ttgtgacaat tcgtccattt aacatttacg gaccgggtca
    10741 ggttggtgaa ggtgccatac agatatttat aaagaatgtt gttaataaca aagacattga
    10801 aattcacggg gacggagatc agatcaggtc ttggtgctat atcgatgata tggtaaatgg
    10861 aatcgaactt tgtattacta ataaaaaagc aatcggagaa gtatttaaca ttggtaatcc
    10921 taaaggtaca atcacaatat cttctttagc cgagaaaata gtttcaatat gtaaatcaaa
    10981 atcgaaaata atttataccc ccaaaaatta tgtagatgtc gaactgagaa tcccaagcat
    11041 tgaaaaagca aaagatcttc ttggctttaa tcccgtagtc gatctgaatg aaggaataaa
    11101 aagaactttc aactggtata aaaaaaatat ctgatgaatt atttttatct ggcacattcc
    11161 gtgatgagtc ttgactgtct taaggatctt gtgtcaaaaa attattcacc cgcacttgtt
    11221 atcattcata aaagccgtga caggaaaaag ctctctgaaa gtttttatag tcccatgata
    11281 gaattctgca gatcaaaaaa aatagaactt atcgaaacag aggatataaa taatttaaaa
    11341 aagagaattg aaaattttaa aacaggtatc tgtgtaggat ttatggatat tctgaataaa
    11401 gattttttcg aaattcctga aaacggtata ttgaatttgc acagcggtaa gctgcccgaa
    11461 tacagaggca gagcacctat aagcagaagt ataatgaatg gagaaaaata cgttacagtt
    11521 tccgttcata aaatagatga aggagttgac tctggtgata tctgcaaaga attaaaaatt
    11581 gagattgctg aagaagatga tgttaatact atttataaaa aatgttcaga atctgcatct
    11641 gttattcttg tagattgtct gaatgaatta aaagggggta aactcgaatt tcataaacag
    11701 gaatctgatg agaaggcgaa caaaattata aataatggag aaagaaaaat taactgggaa
    11761 aataatgcag ttataaatta caataagatc agagctttga cagcaccata tccatgtgca
    11821 tattctattt ttaaaggaag tgaatattta tttttaaaag caaaaacttc tgatgaattt
    11881 aatgataaga atttacagtc tggaacagtt gtaaaggttt ctgataatga aatagttatc
    11941 aaaagcggaa acgggtttct caggatattt aaagtaacag acgagaatct taatgaagta
    12001 aattttaaag aactatttaa tgaaggagat atattcgaat gaaaattata gatgcacata
    12061 ttcatatagg aaagtggagt gacatatttt tcaactatac ttctaccatt gagcaggcag
    12121 ttgacgtaat gaaaaagtca ggtgtcgaag ctgctctttg tatgcctgct gattctactt
    12181 ccaatgatga gcttttcaat atgattaaag attcagattt taaattttat tataatgcat
    12241 ggatagatcc gtctgacaaa gagcttgaca atttccttga taaaaatctg aatgaaattt
    12301 ctgcattcaa aattcatcct tccattcaaa gattaaaaat aactgatctt tcatttaaga
    12361 aatatcttca aatggcatca gatgccggta aaccggtaat tgttcactgc ggaaggtggc
    12421 aggaaatggc aggttttaaa attgctcttg aggctgctgt gaaatttcct gagctgaaac
    12481 tgatactggc ccatatgggc ggtgatcagc cgtctttata tctcgaatgt gctgaagtta
    12541 taaatgactc caaatataaa aatgtatatc tcggtacgga atctgtgcgt gaattttatt
    12601 tcatggatct tgcggtaaaa aaagcaggag cggaaaaagt gatctttgga agtgattaca
    12661 atctgggtct tcctcttact tatattccaa tagtcgaaag tctgaatatt tctcatgaag
    12721 aaaaagagat gatctttaac ggaaatattc taaaactgtt aagttaattt tcttttttaa
    12781 aaaattaaaa accttcagga aaaataaaca agccgtcccc gtcactaatt atttcgatga
    12841 tctctgttac agcgtctata tttagttcac cgtaaatatg cggaaataaa taattattat
    12901 tttctgaatc ttcccatatg atctcactta atactttact ctgatctata aaaagcaaaa
    12961 gtacaatttt ttctttactg aaaaatttaa gtgtagatga ccttacttta tctttaggtg
    13021 aacaatgaat aaatccttca tcttcaagtg aaagagtttt ataagtttta ttcttcttat
    13081 gtttatccca gtgatctatt gtagttatat gataaattaa ttccattaat cttttattat
    13141 ttttataatt tttttgcaat gatatcaaaa acgtgccagt gtttcatttc gccggtagtt
    13201 gtcttgtcat ctttttcaat ctcttcaaag agttcaattt ccattccttc aagtctttct
    13261 aatgcctgat ctttagtatg tctcaccaga tcccattcat cacggtctcc gaagaactga
    13321 ccagagaatc ttccatttct ccttagtgaa tcaacaatgt ttatccatac tttatcgaaa
    13381 tgatttgccg ggcaaaaggg taatgaatag ttagcataga taaaggtggt ctttttccag
    13441 gtaattttct caaaagatac tgcctgaata tctaagttat ttttatgttt ggtcttaaga
    13501 tcatctaaca ttattttaac ttttttattc ttgtctgtgg cagttacact ccatcctttt
    13561 ctgagtaact ctctggtctc atgaccgtta ccacatccga gatcaaatgc atgaccggaa
    13621 tatattttat cttttttaaa aagatcaagt gttctatata aggattcctt tacaggtctt
    13681 tctttatgat gttcataata aacatcccag tcgtattttt ttttcaagta ttaagtttaa
    13741 tttttttgtc caattatatt tactattaca ggtgaaaaaa atatcatgtc aggattttca
    13801 gcatgagatt taagatcatt ccataattct ttttcaagag ttgacgtaat tatattcaat
    13861 tctgttagag attttaaatg acctttgaaa aatatgtatg cccatttaaa aatatcactg
    13921 tcgcatccac ctgcaagatt aaccggtgaa tattcattaa gagaaatatt attttccctg
    13981 aaatattttg gaacctttac agtaaagtaa gggtctcctc caccggcaac ccaatagctt
    14041 tttacatatt cagcaatatt atcaaaaggc cctccctttg gaaaaagagc tataccttca
    14101 taagcataat cctgaattgc aatgactccg ccctttttta atgaacgaaa taatttcaaa
    14161 agtatgtttc ctgcatcagg tacaaaatcg attacccacc tcatgtatat caggttgtaa
    14221 taattctctt caagatccgc attttcaatg tcagagttta taaattttgc gttagagaaa
    14281 ttgtttcttt cacagacatt tctgaaatgt tcaagaaaaa attcacccgg ctctaatgct
    14341 gtaacttcac ctgccccgcc tgtcagttca atcagctgct gtgatacaaa tccgggtccg
    14401 gcaccgacat ctagacattt ccatcctttt tgaatgctga tcttttcaag aaatttatct
    14461 gtaacttctt tccatacacc atgctgaaat tccagtctct caagctctgt ctgattgatc
    14521 ccaagaatgt agtcagtatt ttttccggac attaaaaatt tttgtaaaaa ataacattat
    14581 cagttaaata aaataatgaa ataatttgga atagttttgt gtgaataata aatagtattt
    14641 atagaatgat cccatacaca taaagaaaat gtggatttaa aatcacatcc atccaaatcg
    14701 tttaattgca aaattcaaaa ttctttattt tctacttact ttttttaaac gacaaaaaaa
    14761 gtaacaaaaa aagccacccg ctggtgataa ctctccggaa attctttcgc ttggctacaa
    14821 aaatataaat tccccattcg ttagaaaaac attaacggat tcgtcaaaca atatattttt
    14881 gctaacgcca ttgctcaaaa tttttttccg gcaatttatc acaggcggga tatttagtat
    14941 aagtaaatac tcaaaaaaat attggagggt tagctttttt ttataaatat tagacagcat
    15001 tatttaatat ttaataaact atttttttta acatagttaa ttctttagaa atcaaaagga
    15061 gccatgcatc ggctatgaga atgactctga tcaaataact aaaagttttt gtgatgtcta
    15121 aagtaacaga ttgtaattta atttatgaaa tcctattttg aattcattat tttgacataa
    15181 atacattaat tttagaaaat aaaaatttta acaaaaattt tgttaggatt ttgttagaaa
    15241 gcattttaaa tttgaaatat atttaaattc acataatcat taaaaatcaa actaatcaaa
    15301 atgaaaaatt ctacacctct aaattttagt tttaaaagca tcaatgtttt tgttaaattc
    15361 tgcatgattt tatctttgac tttcttttca atgaaaactg atcagatcaa ggctgatgtc
    15421 gtagctgtca atccgggtag tccaactatt gaagttttta atgaagctac atgggcttca
    15481 atatcatctg caccattaac tatgggcgga tttacaatca caggtgctca ggcagtaaca
    15541 tggaattcag ataatttatt attttatgct attgtaacag ttactccaac agcaagacat
    15601 ttagttacag tagatccggt aaccggaatc tgcacaaatg taggagtatt ggcaagtaca
    15661 gctttttcta cactaacgta taactcaact tcaggaatac tatatacaat gggtggaagc
    15721 ggaggaggac cagttgctga aagattatac agtgtagata taaatactgc agctactact
    15781 ttcctggcag gtccttatac cgttggtggg tttggagaag tcattgcata taactatact
    15841 gacaatatga tctatcactg gtcaggtacc ggtatggaaa tgatcgaccc tgtaactttt
    15901 attgctacac ctgttgtaag gtcaggtgca ccggtagatg aagtcgtagg tgctgtatat
    15961 gtaggcgggg gtatattcca aataactgat cttgtattta cagttagtac aagtgcattc
    16021 acgatgacat ctgcaggtgt tgctacttta atcggaccga catcatacag agcaagaggt
    16081 ctgggatatg tcgatccatt actaccggtt gaattgtctt cttttagttc ttcaataagc
    16141 ggaagtgatg ttacattaaa ctggtcaact tcttcagaga caaataactc aggttttgat
    16201 atcgaaagat catctgtttc cggtacctgg tcaaaagttg gaaacgtaac cggtaacggt
    16261 acttccatta ctacaaataa ttattcttac actgacaaaa atttaacatc aggaaattac
    16321 agctacagac tgaaacagat agacttcaac ggtaacttcg aatatttcaa cttaagtaat
    16381 gaagttattg tcggaattcc tgtgaagtat gacctttcac aaaactatcc aaatcctttc
    16441 aatccatcta cgaagataaa ttatactctt ccgacagagg gattagtaag tattaagatt
    16501 tttgatatgt ctggaaaaga agtgatgtca cttgtaaatg aagtaaagac tgctggttat
    16561 tattcagtta actttaatgc ttctaactta tcaagtggaa tttattttta tagtttaagt
    16621 gcagcagact ttacagatac aaagaaaatg atgcttgtaa aataagttaa tagttaatag
    16681 ttagtaattt tttactattt gctatttaat atttaatatt taagatttaa aattaaaaag
    16741 ccgttggatg tagaatccgg cggctttttt ttatcatcac tttgcagaaa actatttttg
    16801 atagggtgaa ttgaaattac taaacaacta attttaaatt aataaaaata tattgtaagg
    16861 aattttaatt agcaattttc aatccgacaa tgccgatcac aatcagggaa gctgagagta
    16921 atctccagat actggctgag tcaccgaagt aaattatccc aacgataagt gtaccaacag
    16981 ctccgatacc tgtccagact gcgtatgctg tgccaatagg aatatccctt tgagccaacc
    17041 acagaaataa cccgcttatt gccatggaaa taactgcaag aattatccat aatattttgg
    17101 aagacattgt ttgagataat ttcataccga gtggccagcc gatctcaaaa ataccggcaa
    17161 ttaataaata gatccagctc ataaattaat ttaatttaat ttttttaaaa aagcatctga
    17221 tttgatttta aaaactgttc atgttttttt atatatttaa tttgatttaa tattgattta
    17281 atattgattt gatcaaagta aataatttat caataccagt aacccatcat atctaattcc
    17341 ccatcgtcct tccattcttt atctcctaac tgctttatca tcaggcaatt gtcaaattcc
    17401 ttttcctgta ttccataatt aatatgctct tcaatagtca gccatttcac tttttgtttt
    17461 gtaagtctgt aactgcatct ttcataaaat ttatgatagt cagcgacaag gaataaaaaa
    17521 tcaatattat gtatatgctt gtcgattata ttttcaagtt ctttcagaag atgagtacca
    17581 ttaccctgat gctgaaactc aggtaatatc ataagatcaa ttattcccat tactctaaca
    17641 ggatctttat tcaatgtcat tactctgtaa tcaagtccaa gctgtccaat gagaatatcg
    17701 tctttccaca tcaggagacg atagtgagga agctgtttga aataagtcct tccatgaaat
    17761 tcttcttcag gaaaactaat cttcagaagg gcggatatct gaattttagt atttagatcc
    17821 agtttgaatt catcaattaa ttcaaatcga aattttgaaa tcgtattcaa aatataatat
    17881 tttaatttct aaataataaa aataccagta ctacaagaga gattatcacc cgatcgaaag
    17941 aatataaatt atgtatttaa atctgttttt attcggatca gatctttttc catttttcca
    18001 aaataccata agacaagact cattattcca aatgtaaaag aatgaaataa ttagtaaaca
    18061 tcattcttaa taatcttaat aattctaaaa tgaaaatttt gaaaaattta ctatgtacat
    18121 ttttaaaata gcaaatatat ctatctgaat aagtaaagta aattttaatt tatgggaaat
    18181 tatttttcct cccgtgaaat ttttaaatta actactgaaa tttacttatg aataaattaa
    18241 aatatgacac aggtatgaca attatgtctt agataaatga agtcaggatt ttaaagtgct
    18301 tttaatggaa ccaaagggga aattattatt ggcacaatga ttgcattata tgatattaaa
    18361 caaagctata agataacatt gaaaatatta aatctttagt ttcattgaga aagatgaaaa
    18421 taaaagtaat aaataaatag cattttatta aataaaaaaa aataaaaatg aataacgaaa
    18481 aagcaattga agtactgaat tctctcgttg aaattaataa tgacaggatt gaaggttaca
    18541 aaagtgcaag tgaagaaaca gattcaaatg atttaaaggg attatttatg cagttcaaga
    18601 atacaagtga aagatgtatt acagagctga caaaagaaat aatcaagtgc ggcggtattc
    18661 ctgaagaagg tactaaaaca tccggaaaac tttatcgtgc atacatggat ctaaaagcag
    18721 ccttgacagg aaatgatcag cagaaaatac tggattcatg tgaatatggt gaagacaaag
    18781 ctgtagaaac ttataaagat gtatttaata ataattcaga aaatctgaca gctgaacaaa
    18841 tttcattggt aagatcacaa tatgatctta ttaaaaacga tcatgacaca gtaaaatcaa
    18901 tgcgtgacag tgaagtaaca ttgtaatcaa tttgatcaat tagactaaat agtgtgttga
    18961 ttgatcgatg atccttaagg cgatctgctt aattattatt gtaaattaat atgaagatta
    19021 agatcttgat tcttacaatt tcataggttt caata
//
LOCUS       DAORVQ010000042        18750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1207_length_18750_cov_5.635518, whole genome shotgun sequence.
ACCESSION   DAORVQ010000042 DAORVQ010000000
VERSION     DAORVQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 18750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18750
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1207_length_18750_cov_5.635518"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..2259
                     /gene="typA"
                     /locus_tag="PLX80_05275"
     CDS             391..2259
                     /gene="typA"
                     /locus_tag="PLX80_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQY20240.1"
                     /translation="MKNLLFNNKLRNIAIIAHVDHGKTTLVDHMFRQSGMFRENQEIA
                     ERLMDSMDLERERGITIAAKNCSVTWGDTKINILDTPGHADFGGEVERALMMVDGVVL
                     LVDASEGPLPQTRFVLKKALESNLKIIVIVNKIDRKDARPEEVLNEVYDLFIDLDASE
                     EQIEFPVLYAIGRDGIAQKNLSDNGKDLSILFETIIEEITPPSYDPDEPFQMLVADLD
                     YSDYVGRLAIGKIANGEIKKNDELVCINEAGKALPLKISKLQIYDGVNFKESDIVEAG
                     DIAILAGIEDVHIGDTICNKEFPKALRRIAVDEPTISMMFSINTSPLSGREGKIVQSN
                     KIRERLMKETLSNVALKVEDTKEGDSFIVKGRGEFQMVILIETMRREGYELSVGRPQV
                     IFRRIDGKLHEPIEHLFIDCDENFVGVVSEKLSKRKGRMINLVNHGTGRVRVEFSIPS
                     RSLIGYRNEFLTDTRGTGIMNSYLQGYEEHRGEFPARLTGSLVSDRMGEALSYSLYNL
                     EPRGTLFSIPNDQVYEGMIVGEHNRDNDLDVNPTKGKKLSNMRASGKDESMILTPVTP
                     MTLEKAIDFIKDDELVEVTPKNIRLRKSILSSQTRHTNRGKAFQKLGEVIQELNGI"
     gene            2249..2599
                     /locus_tag="PLX80_05280"
     CDS             2249..2599
                     /locus_tag="PLX80_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018300344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQY20241.1"
                     /translation="MGSKSLDEKVEKLGIGKTKRHIFLCCDQGKAKCCDYKDGMKSWE
                     YLKDRLKELGLVNEGGINRTKANCLRICLDGPLAVVYPEGVWYRNCTPEVLERIIKEH
                     LINGNIVEEYRIKI"
     gene            2643..4343
                     /locus_tag="PLX80_05285"
     CDS             2643..4343
                     /locus_tag="PLX80_05285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HQY20242.1"
                     /translation="MKFNEFEYKRPDIESVKKDFNEKLDKFRNSGSAEEQYSVMKEIT
                     EIRNDYDSMGRIANIRYTIDISDKFYEEEHDYFDDIGPGYSGLVKEFYFELINSPFRK
                     ELEEICGMQLFNVAEVSVKTFKPEIIEDMKKENHLSSKYVRLIASAKIMFEGEERNIA
                     GMASFMNSDNREMRKRAYDAKWKFFEDNEKEIDDIFDELVKVRTKIAHALGYENFIQL
                     GYDRLKRTGYSSTEVAGFRDEILKFLVPLTTELKELQLQRTGLDKLYYYDSEYNFKNG
                     NPTPKGSPDWIVEKAKIMYDELSPETSEFFDFMIDHDVMDLYNRKNKSAGGYCSYIQK
                     YQSPYIFANMNGTDHDIKVLTHEAGHAFQGYESRKFEFPEYQHPTLEACEIHSMSMEF
                     ITWPWMNLFFEVDTDKFLFSHLNRALMFIPYGVTVDEFQHFVYSNPDATPQERKIKWR
                     EIEKKYMPHKNYDGNDFLSRGGYWFMQGHIFKMPFYYIDYCLAEICALQFWRKCNHDK
                     DIAWKDYLGLCREGGSKPFLELVEIANIESPFESSVVESIVNYSEEWLENVSKEYDLV
                     "
     gene            complement(4534..6075)
                     /locus_tag="PLX80_05290"
     CDS             complement(4534..6075)
                     /locus_tag="PLX80_05290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY20243.1"
                     /translation="MTHKIFKDRSQLFLVLILSSIPFLIHLYVNIFEGYGYFRDELYY
                     IVSTEHLSFGYVDHPPLSIFILYISKTIFGDSLFAIRLLPALLSGLTVFFTCLMALRL
                     GGKTFSLTLSAAAVILAPVYLAMNSFYSMNSIDIFLWSVSFYLVMLIIKESKTSYWIY
                     LGLFIGLGLMNKIGFLWFEFGFFTGILLTHKRKEFLTVKPYLTLIISFVIFLPYIIWN
                     FQNDFAHLEFIRNASSEKYSGLNITDFIKGQFMNMNPFSVVIWLCGLYYFIFNEKGKE
                     FRIIGIIYLTAFIILIINVHSKSEYLAPAYTVLFAGGSVFIELKSQMKFKWLRFAVLI
                     PLLITGVVIAPTVMPLLKVEDHISYTKNLGLAPSSSEGKKLSELKQFFADMHGWEEMA
                     ANVSKVYESLSAEEKQNTLFFAWNYGEASAINFFRNKYPLPEAISSHNAFWMWGYGDK
                     EFPTLIIIGGEKEDYTDEFGSVVEVGIHTSKYAMPYETDLMIFIAKDPKNQLSEVWQR
                     IKHYD"
     gene            6189..7088
                     /locus_tag="PLX80_05295"
     CDS             6189..7088
                     /locus_tag="PLX80_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQY20244.1"
                     /translation="MKTISPYRVLLYYKYVKIVDHETYSKLHLKFCKSLGVKGRILIA
                     EEGINGTLSGTPEQTDAYIYAMRMDPRFTDMTFKIDDVEEHAFKKLFVRSRSEIVTMD
                     LENDLDPNQITGKRLKPKQFLEAMNDENVLLIDVRNDYEYDIGHFKNAVRPDVKNFKQ
                     FPEWAKKNKDLLKDKKILAYCTGGVRCEKFSGFLVREGFTNVNQLKGGIIAYAKHPDT
                     RGSNFEGKCYVFDERIAVDVNFAEGTSIVGKCLRCENPSDRYVNCANFKCHVHFICCE
                     ECDEKFHKTCSEKCDKEQLSIKN"
     gene            complement(7178..8362)
                     /locus_tag="PLX80_05300"
     CDS             complement(7178..8362)
                     /locus_tag="PLX80_05300"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY20245.1"
                     /translation="MQKRDVIIIGAGAAGLMCAAEAGKRGRMVTILESSEKPGKKILI
                     SGGGRCNFTNRIVTSENFISNNPHFSRSALAGFTPDDFIFMVESYNISYHEKKLGQLF
                     CDGSSREILYMLTDECKKYGVEIITDCKISEVIKIESNSFEIRSSYKNFVCDSIVIAT
                     GGLSIPKMGATDFGYRIAKKFEINIINTFPALVPLKFNVNEWKEFSDISGVSVDSIVS
                     VKSKGKIKFRENVLFTHKGLSGPAILQISSYINNTESFEIDLLPDRDMFKIFIENSGS
                     KILLNNFLSAYFPKRFSEAFVGKYILSKPVYQYTQKDLKNISDKIHNWELQPSGTEGY
                     EKAEVTAGGIDTRELSSKTMESKKVSGLYFIGEVVDVTGWLGGYNFQWAWSSGAAAGR
                     SC"
     gene            8446..10260
                     /locus_tag="PLX80_05305"
     CDS             8446..10260
                     /locus_tag="PLX80_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20246.1"
                     /translation="MNLYESRLDYLQKLSVKLNDRIEYLKKKSNMFSFNRLWIFLSGL
                     VLSVTAFNIDQTIGCIVSAISILTFGITVHLHNRLLHTVKRISYFRILQNENISRMKL
                     DWKGIPGNVSGIIPDESSTFKDLDITGKRSLHQLLDNSVSFEGSSLLSKKLSQFSPDI
                     KKISRNQKIIKELSGMKRFREKFILKARLISLKPLNGTAILNWLKKSEHSTIPDFVIP
                     VSIIFILSYLILFGLYIAGILGSIWFAVFLMYLIFYGKYQKPASSVFEESALLCDHIR
                     KFSVLMQLAGKYNMKNFPETEKFLDIFKAEKDGASDELIKLDKLLMLIRLRDNPIYRV
                     IFNAFIPYDFIVCKKLLTLISDIEHKLPEWLDKFYELECLISVSNFSYLNPDYNYPEF
                     ETEREFTFEIKECGHPLLQRDQKICNDFNFSKESETVIITGSNMSGKSTFLKTVGINQ
                     CLANAGAPVNAISFKTSVYDLFTCIKVNDSVADGISYFYAEVRRLKELIDEIKISKNK
                     IKFFLIDEIFKGTNNKERLHGSREFIRYLSKQNCAGFITTHDLELVNISDEIDTVLNF
                     HFREEILNNEMIFDYKIHNGPCPTTNALKIMEIAGLPV"
     gene            10473..10961
                     /locus_tag="PLX80_05310"
     CDS             10473..10961
                     /locus_tag="PLX80_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20247.1"
                     /translation="MNPEIKEEIKKITDKIVEIKKNKGDSNSELIATENSTKFKELIT
                     NHIIPLVKESNMIIGRSGCSMNYYSNELAVIRDPLKRIFIQILFYPYGHSKITLGVNA
                     PFLQFAYSPLKENIKITQKLSIKSDEPRIKVDEIEINDIAMDKINQYFLDFLNETIVY
                     NK"
     gene            11097..13331
                     /locus_tag="PLX80_05315"
     CDS             11097..13331
                     /locus_tag="PLX80_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQY20248.1"
                     /translation="MHSIFANGKYIPTSKNSDVKNEVRLISSDNNNTVISVRVAGYSI
                     VERSVTGENFDAINIEGYTSLGNTGQASLPVMTKMISIPNFKNVSIKILNSSEKNYSV
                     NKIYPSQEFPLRNSNAEVKDFSYDKNYYTSDKNYPSEIVSIKEIAVMRDHRVAVISIN
                     PVRYNPAKNEMTVYDEIEFELVYDGYSNVNNVTNSVYSRSKSFENIYKNTIFNYGSIN
                     NDNTFLIAPKMLILTADSLYNSILPFAEWKNQKGIKTTVTLRSEINANGNPTSTEIKN
                     YLTANYNSADRTEYVLIVGDARGRNTLTWFNATGGKSDHPYQCLDGTDILPDIVVGRI
                     SVQSVSELDSALASLMQYEKFPEMDDTDWYKRALVLHSNDGIDPINGQVAKSVFLNEG
                     GFTNVDIAMESTSQNQITSFINGGVSWTWFIGHGNETSWASPVWNMSNMVNLNYENRK
                     PTIISIACSNADLDYSETNDCFGEAWIERSTKNTAINIAASTELCSFYTSDTIGREML
                     YGYFRHGIYDFGSMLNFGKIQAYNYFNGSGSVIATINQFMVLGDPSQEAFSDVPKNIS
                     LLRDYSGGQHKFNIKTGSENYNGALIAISQNSELKSSGYTDSLGNYSFNSDLINNNLP
                     VNIVVTGKNLNPYFGNMILTSISSGVENPNKFSLNQNYPNPFNPYTIISFSIGNNLNL
                     SENVILKVYDILGNETATLVNERLTSGSYSIEWNAGNFSSGVYFYKLSAGNFSEVKKM
                     TLLK"
     gene            complement(13455..13880)
                     /locus_tag="PLX80_05320"
     CDS             complement(13455..13880)
                     /locus_tag="PLX80_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20249.1"
                     /translation="MNSLNNFIRKTSKPLMIIFGLATCLPLVFAFNINLLPDFAEGIE
                     FTASSSPALRHWGFMVFGIGALLIAAAFYPWLRFYTMLFSILEKSFMVFLFISNINEP
                     WGSAYTGMGIMDSIIVFYSLLYFISSEGRPGKWVLADKK"
     gene            14168..15586
                     /gene="atpD"
                     /locus_tag="PLX80_05325"
     CDS             14168..15586
                     /gene="atpD"
                     /locus_tag="PLX80_05325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQY20250.1"
                     /translation="MSNNTNNKGKILQIIGPVLDIDFSGGSLPAINNAVEIKRKNTEG
                     VDEVLITEVQQHLGESRVRTVAMDSTDGLVRGMEAIDTGIPISIPVGEEVLGRLINVT
                     GGTIDGKGPINTDLKYPIHRPAPKFSELSTKKEMFETGIKVIDLLEPYTKGGKTGLFG
                     GAGVGKTVIIQELIHNIAKHHGGYSVFAGVGERTREGNDLYLEMEESGVLKQTALVFG
                     QMNEPPGARQRVALTGLAIAEYFRDQGKDVLLFVDNIFRFTQAGSEVSALLGRMPSAV
                     GYQPTLASEMGALQERITSTKTGSITSIQAIYVPADDYTDPAPATTFAHLDATTELSR
                     KISELGIYPAVDPLTSTSRILDPLVVGEDHYNTAQGVKKILQTYKDLQDIINILGIDE
                     LSDEDKLTVARARKIQKFLSQPFFVAEQFTNLPGKYVKLEDTIKGFKGIINGEYDDLP
                     ENAFYLVGTIEEAVEKGKKLSE"
     gene            15645..16055
                     /locus_tag="PLX80_05330"
     CDS             15645..16055
                     /locus_tag="PLX80_05330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQY20251.1"
                     /translation="MDQNLYLEIVSPVKMVYSEEVDSVTIPGTDGSFQVLKNHAALIA
                     TFSLGKIKIKKGQETIIYSTSGGIFEVKNNKAIVLAESIETVEEIDIDRAKLAKTRAE
                     EILAMAEANEDEVRDAKDALQRAINRLKIAEKGK"
     gene            16074..16145
                     /locus_tag="PLX80_05335"
     tRNA            16074..16145
                     /locus_tag="PLX80_05335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16106..16108,aa:Lys,seq:ttt)
     gene            16310..18028
                     /locus_tag="PLX80_05340"
     CDS             16310..18028
                     /locus_tag="PLX80_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20252.1"
                     /translation="MKKFFLILCFVYTAFQSSANASPRISFEDLLIQSELNPQNVLQA
                     RETAIRQGIPVSIMTSDRIMIDAKYVEDGRPVYAVYTNFADIYNGGYAAYYEDVVSKI
                     NFANARIDYGNRNIVDNTNGFFTPVLSGRNAASSFIMITESTSDRVSIFNAETGDLID
                     TAFIPSTRPQLGTPKVALKHYANSNVIICDQITDVVQDFNLNGTYKGFYAPSTGPNTS
                     IVDNMRGMRYRSNNNIVVSVGSGVNQNTIQQFDPSGNHIGTYITGNLNSPFDILIRAN
                     DVLVSNFSGTNRVSRYDSNGVFLNSFYTGSNFGISQQMYEVAANGNVAVSSFQTPSGL
                     AILNSAGAFVKLLNGVTGNRGVHLLGNGNFLVTNSTGAHEIDSASGSLVRTIHLSPNF
                     QYATKYEIPNPYLRLRINLEACAILDTVNVDIRNSSSPFSIIESKKVVAGLGNSVDVQ
                     FNTPVNGTPYYIQVSHRNSIATWSGSTPSFNSDYINYTFISASSQAFGSNMVNVGGKW
                     SFYTGDVDQDGVVDVTDVGLIDNDSFNFASGYLSTDLDCNDIIDITDLAYADNNAFNF
                     VSEVAP"
     gene            complement(18285..>18750)
                     /locus_tag="PLX80_05345"
     CDS             complement(18285..>18750)
                     /locus_tag="PLX80_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20253.1"
                     /translation="LKVGQFDWAYDFILNCKNNPDQTDSADLSRYGYLNFLFYKGEFK
                     ECLNNCSLASLKNITLKIGVSKITLKVMYELGYFDSARSFLSNYKHYLNNSSNLSINI
                     GSREIQFTNYYQILLNNTVSSKKTYLKNIIMDLKSETNIADKDWLMSKFQNV"
BASE COUNT         6632 a         2883 c         3431 g         5804 t
ORIGIN      
        1 attcttgaga aattgaaaaa ataaaatatc cttgtctgag aaatttgcga agcaaatttt
       61 gagttggata ttttatttca atttcgaaaa gaatttaggc agtatattac cagcgggcgt
      121 ctttttttgt tacttttgga gcctgctctc gagtgttctt atcgagggtc tttataaaaa
      181 agttagtaga aaataatgaa acttgagttt cacaatgaaa cgtgttggac ggatgtgata
      241 tgaaatataa atcatttaag gtgctaattc tcacttgtaa cactcactta ataaagctat
      301 tttacatact ttgaaatcag taataccttc cgacataaat ttccaaaaaa ctgaattaac
      361 tatttataaa accccaataa aaaattataa ttgaaaaatc ttttgtttaa caataagtta
      421 agaaatatag ctattatagc tcacgttgat catggtaaga ctacacttgt agatcatatg
      481 ttcagacaaa gcggaatgtt ccgtgaaaat caggagatcg ccgaaagact tatggacagt
      541 atggaccttg aaagagaaag agggatcacc attgccgcta agaattgctc cgttacatgg
      601 ggcgatacaa agataaacat tcttgatact cccggtcacg ctgatttcgg cggcgaggtc
      661 gaaagagctc tgatgatggt ggacggcgta gtgcttttag tggatgcatc tgaaggtcca
      721 cttcctcaga caagatttgt attgaaaaaa gctctggagt caaatttaaa aataatcgta
      781 atcgtcaata agatcgacag aaaggatgcc agaccggagg aagttttgaa tgaagtctat
      841 gatcttttca tcgaccttga tgccagtgaa gaacagattg aatttcccgt gctttatgct
      901 attggaagag atggtattgc tcaaaaaaat ctttcagaca atggaaagga tctttctata
      961 ttatttgaaa caataataga agaaattact cctccatctt atgatccgga tgaaccgttt
     1021 cagatgcttg ttgctgatct tgattattca gattatgtag gcagacttgc cattggtaag
     1081 attgcaaacg gagaaataaa aaagaatgat gaacttgttt gcatcaatga agccggtaaa
     1141 gcacttccat taaaaatatc aaaacttcag atctatgacg gagttaattt taaagaatct
     1201 gacatagtag aagcaggcga cattgcaata cttgcaggta ttgaagatgt tcatatcggt
     1261 gatactattt gtaataaaga atttcctaaa gctctaagga gaattgctgt agatgagccg
     1321 actatttcaa tgatgttttc tataaatact tcgccgcttt caggcaggga aggaaagatc
     1381 gttcagtcaa ataagatacg tgaacgtctt atgaaagaaa ctctcagtaa tgttgcttta
     1441 aaagtagaag atacaaaaga aggtgatagt tttattgtaa aaggtcgcgg tgaatttcag
     1501 atggtaatac ttatagaaac aatgagacgc gaaggatatg aactgagcgt aggcagaccg
     1561 caggtgatct tcagaagaat tgatggaaag ctacatgagc cgatcgaaca tttgtttata
     1621 gactgtgatg aaaattttgt aggggttgtt tcagaaaagc tctcaaagag aaaaggaaga
     1681 atgatcaatc ttgtaaatca tggtaccggt agagtaaggg tcgaattttc aattccatcc
     1741 agatctctga tcggatacag aaatgaattt cttacggaca cacgcggaac aggaataatg
     1801 aattcttatc ttcagggtta tgaagagcat cgcggagaat ttcctgccag acttacagga
     1861 tctcttgttt ctgacagaat gggagaagcg ctatcttatt cactttataa tctcgagcct
     1921 agaggaacat tattcagtat tccaaatgat caggtctatg aaggaatgat agtaggggag
     1981 cataaccgtg acaatgatct tgatgtaaat cctacaaaag gtaaaaaact ttcgaatatg
     2041 agagcatcag gaaaagatga aagtatgata cttacacctg taactccgat gacacttgag
     2101 aaggcaattg atttcataaa agatgatgag ttggtggaag ttaccccaaa aaatataaga
     2161 ttaagaaaat caatcctttc ctcacagaca aggcatacca acagaggaaa ggcatttcaa
     2221 aaattaggag aggtaataca ggaattaaat gggatctaaa tcattagacg aaaaagtaga
     2281 aaagcttgga ataggaaaaa ccaaaagaca tattttcctt tgctgtgatc agggaaaagc
     2341 aaaatgttgt gattacaaag acggaatgaa gtcatgggaa tatctaaaag acaggctaaa
     2401 agaacttgga cttgtaaatg aaggcggcat aaacagaaca aaggcgaatt gtctcagaat
     2461 atgtctggac ggacctttag cagtagtgta tcctgaaggc gtctggtaca gaaactgtac
     2521 ccctgaagta cttgaaagaa taataaaaga acatttgata aatggtaata ttgtagaaga
     2581 atacagaatt aaaatttaaa aataacttat aacatcttta cagaaaggaa attattttgg
     2641 caataaaatt taatgagttt gaatataagc gtcctgacat tgaatcagta aaaaaagatt
     2701 ttaatgagaa gctggataag tttagaaatt caggtagtgc tgaagagcag tattctgtaa
     2761 tgaaagaaat tacagagatc agaaatgatt atgactcaat gggacgaatt gcaaatatca
     2821 gatatacaat tgatatttcc gataaatttt atgaagaaga gcatgattat tttgatgata
     2881 tcggacctgg ctattcaggg cttgtcaaag aattttattt cgaattaata aactcacctt
     2941 tccgaaaaga gcttgaagag atctgcggta tgcagctttt caatgtagct gaagtttctg
     3001 taaagacctt taagcctgag atcattgaag atatgaaaaa agaaaatcat ctttcatcaa
     3061 aatatgtaag acttattgct tctgcgaaaa ttatgtttga aggtgaagaa agaaatattg
     3121 ccggcatggc ttcttttatg aattccgata acagagaaat gagaaaaaga gcttatgatg
     3181 ctaaatggaa attctttgaa gataatgaaa aagaaattga tgatattttt gatgaacttg
     3241 taaaagtcag aacaaagata gcacatgctc ttggttatga aaattttata cagcttggat
     3301 atgatagatt aaaaagaaca ggatattcta gtactgaagt agcaggattt agagatgaaa
     3361 ttttaaaatt ccttgtccct ttgactacag aacttaaaga acttcagctt cagagaaccg
     3421 gtctggacaa actttattat tatgacagtg aatataattt taaaaatgga aatccaactc
     3481 caaaaggttc acctgactgg atagttgaaa aagcaaagat aatgtatgat gaactttctc
     3541 ctgagacttc cgaatttttt gatttcatga tcgatcatga tgtgatggat ctttataaca
     3601 gaaaaaataa atcagccggc ggatattgtt cttatattca gaaatatcaa agcccgtata
     3661 tctttgcaaa tatgaatggt acagatcatg atataaaagt tcttactcat gaagcaggtc
     3721 atgcttttca gggatatgaa agccgcaagt ttgaattccc tgaatatcag catccgacat
     3781 tagaagcttg tgagatacat tcaatgagta tggaatttat tacctggcca tggatgaatt
     3841 tattctttga agtggataca gataaattct tattctccca tcttaacaga gcactgatgt
     3901 ttattcctta tggtgtaact gtagatgaat ttcagcattt cgtttattca aatcctgatg
     3961 caactcctca agaaagaaaa ataaagtgga gagagatcga gaaaaaatac atgcctcata
     4021 aaaattacga tggaaatgat tttctatcaa gaggcggtta ttggtttatg cagggacata
     4081 tttttaaaat gccgttttat tatatagatt attgtctggc agagatctgc gcgttgcaat
     4141 tctggagaaa atgtaatcat gacaaagaca ttgcatggaa agattatcta ggtctttgca
     4201 gagaaggcgg cagtaaacct tttcttgagt tagtggaaat tgcaaatatt gagtcaccgt
     4261 ttgaatccag tgtagtggaa tcgattgtca attattctga agaatggtta gaaaatgtaa
     4321 gtaaagaata cgatcttgtc tagataaatt ttacaaaatt aatttatatt aaataagacc
     4381 attctgttaa ttttagaatg gtcttattta ttttaataag tactacactt attgattcac
     4441 acttaaattc gctccgaaaa gcacgtgatt ctgcgggaac agttgtaatt gattccgggg
     4501 gactgcatta taatttataa atcgaaattg agattaatca taatgtttta tcctttgcca
     4561 gacttcactc agctgatttt taggatcttt tgcaataaag atcattaggt cagtttcata
     4621 aggcattgca tatttggatg tatgaatccc aacttctaca acggatccaa actcatcagt
     4681 ataatcttct ttttcgcctc cgattatgat aagtgtggga aattctttgt caccataacc
     4741 ccacatccag aaagcattat gagatgagat cgcttcagga agcggatact tatttctgaa
     4801 gaaattaatt gccgatgctt ctccataatt ccatgcaaag aaaagtgtat tctgtttttc
     4861 ttccgctgaa agtgattcat aaactttcga tacatttgct gccatctctt cccagccatg
     4921 catatctgca aaaaattgtt taagctctga aagttttttc ccttccgaag aagaaggagc
     4981 taatcctaaa ttttttgtat aagaaatatg atcttctact ttcagcaaag gcattacagt
     5041 cggagcaatt acaactcctg ttatcagtaa cggaattagt acggcaaatc tcagccattt
     5101 gaatttcatc tgtgatttta attctatgaa tactgaaccg cctgcaaaca gaactgtata
     5161 tgcaggtgca agatactcag atttactgtg aacattaatt atcaatatta taaatgcagt
     5221 taaataaata ataccaatga tcctgaattc tttacctttc tcattaaata taaaataata
     5281 aagtccgcac aaccagataa caacagagaa aggattcata ttcataaact gacctttaat
     5341 aaaatctgta atatttagcc ctgaatattt ttctgagctc gcgttcctta taaattctaa
     5401 gtgcgcaaag tcattttgaa aattccatat tatataagga aggaaaatta caaaagatat
     5461 aattaaagtc aggtaaggtt ttacagttaa aaattctttc cgcttatgag ttagcagtat
     5521 tcctgtgaaa aatccaaact caaaccagag gaaaccgatc ttgttcatca gtccaagtcc
     5581 gataaacaaa cccagataaa tccagtaaga agttttactt tcttttataa taagcattac
     5641 caaataaaaa gaaacactcc agaggaatat gtctatagag ttcattgagt aaaaggaatt
     5701 cattgctaaa tatacaggtg caagtataac tgcagcggct gacagtgtta gtgaaaaagt
     5761 tttacctcca agtcttaatg ccataaggca tgtaaaaaat acagtgagac ctgacagcaa
     5821 agcaggtaat agccttattg caaataatga atcgccgaaa atagttttgc ttatgtacag
     5881 aataaaaata gacaatggag gatgatcgac atatccaaaa gagagatgtt cggtactgac
     5941 aatgtaataa agttcatccc ggaaatatcc gtatccttca aaaatattta catataaatg
     6001 tattaaaaac gggatagatg aaagtataag aacaagaaac agttgtgaac ggtctttgaa
     6061 aattttatga gtcaaggata aataaatatt aaaaaatttt taattaaaat aatgaaatca
     6121 taaattaaaa aaacggtatg acgatattta taaattgaat aataaacctg atagaattaa
     6181 ttttacatat gaaaacaatt tcaccttaca gagttttact ctattataaa tatgtcaaaa
     6241 ttgtagatca cgaaacctac tcaaaacttc atttgaaatt ctgcaaatca ctcggtgtaa
     6301 aaggcagaat tctgatagca gaagagggga tcaacggaac gctttccggt acgcctgagc
     6361 agactgacgc ttacatttat gcaatgagaa tggatccgag atttactgac atgacattca
     6421 agatcgatga tgtagaagag cacgctttca aaaaattatt tgtcagatca agaagtgaga
     6481 tcgttactat ggatctggaa aatgatctcg atccaaatca gatcaccggt aaaagactta
     6541 aaccgaagca atttctcgaa gcaatgaacg atgaaaatgt attattgata gatgtaagaa
     6601 atgattatga atatgacatt ggacatttta aaaatgctgt cagacctgat gtgaaaaatt
     6661 tcaaacagtt tcctgaatgg gcaaaaaaga ataaagatct attaaaggat aaaaaaattc
     6721 tcgcatactg tacaggcgga gttcgatgtg aaaaattttc aggctttctt gtgcgggagg
     6781 gttttacaaa tgttaatcag ctaaagggtg gaatcattgc ttatgcaaaa catccggaca
     6841 cccgcggtag taattttgaa ggaaaatgtt atgtgtttga tgaaaggatt gcagtggatg
     6901 tgaattttgc tgaaggtact tcaatagtgg gaaaatgttt acgctgtgaa aatccttcag
     6961 acagatatgt aaactgtgca aactttaaat gccatgttca ttttatttgt tgtgaagaat
     7021 gtgatgagaa atttcataaa acatgcagcg aaaaatgtga caaagagcaa ttaagtatta
     7081 aaaattaaga attaagaatt ttgttaatgt gatttataga ttagcaattc gtaacttgaa
     7141 actcgtaact cgtaatttct aatttctaat tcctaattca acaactcctt cctgcagcag
     7201 ctcctgacga ccaagcccat tgaaaattat atccgcctag ccaacctgtg acatctacta
     7261 cttctccgat gaaataaagt ccgcttactt tttttgattc cattgtcttg gaggaaagtt
     7321 ctctggtgtc aattccgccg gcagtgactt cagctttttc atatccttca gttcccgacg
     7381 gttgtaattc ccaattgtgg atcttatctg aaatgttctt caaatctttt tgggtatatt
     7441 ggtaaactgg ttttgataaa atatatttcc ctacaaatgc ttctgaaaat cttttaggga
     7501 aatatgcaga aagaaaatta ttaagcagta ttttacttcc ggaattttca ataaaaattt
     7561 taaacatatc cctatcaggt aaaagatcaa tttcaaagct ttcagtattg tttatgtatg
     7621 aagagatctg aagaatcgcc ggaccactga gacctttgtg tgtgaagagt acattttccc
     7681 ggaattttat cttacctttg gatttgactg aaactattga atcaactgat actccactga
     7741 tatccgaaaa ttctttccat tcattaacat taaatttaag cggcaccagt gcaggaaaag
     7801 tattaataat atttatctca aactttttag caattcgata tccaaaatct gtagctccca
     7861 ttttaggaat tgaaagtccg cctgtagcaa ttacaataga atcacaaaca aaatttttat
     7921 aagatgatct tatttcaaaa gaattacttt caatttttat aacttctgaa attttacagt
     7981 cagtaataat ttccacacca tattttttac attcatcagt aagcatgtaa agaatttctc
     8041 tggaacttcc gtcacaaaaa agctgaccga gttttttttc atgatagctg atattatatg
     8101 attcgaccat aaaaataaaa tcatccggtg taaatcctgc cagtgctgat ctggagaaat
     8161 gaggattgtt cgaaataaaa ttttcagaag tgactattct gtttgtaaag ttacaccgtc
     8221 caccgcctga aataaggatc ttctttccgg gtttctctga actttcgaga attgtaacca
     8281 tccttcctct tttgcctgct tcggctgcac acatcagtcc tgctgcaccg gctccaatta
     8341 ttatgacatc cctcttttgc aataagtaat tattaaaatt tataatttgt catttcgtaa
     8401 aaattaagaa atgtaattta agcaatataa attttatatt taattatgaa tttatatgaa
     8461 agcagattag attacctgca gaaattatca gtaaagctaa atgatagaat agaatatctc
     8521 aaaaagaaaa gtaatatgtt ctcttttaac agattatgga tatttcttag cgggttagtt
     8581 ttgtcagtta ctgcgtttaa tattgaccag accatcggat gcattgtatc agcaatttct
     8641 atactgactt tcgggatcac agtacatctt cataacagac tattgcatac cgtaaaaaga
     8701 atatcgtact ttagaatttt acagaatgaa aatatttccc gaatgaaact tgactggaaa
     8761 ggtattcccg gtaatgtcag cggaataatt cctgacgaat catctacatt taaagatctt
     8821 gatatcacag gaaagagatc tcttcatcag ctattagata attcagtttc tttcgaagga
     8881 agttcattac tatcaaagaa gctgtcacaa ttttcacctg acattaaaaa gataagcaga
     8941 aatcagaaaa ttattaaaga actttcagga atgaaaagat tcagagagaa atttattttg
     9001 aaagccagac ttatatcttt aaaacctctt aacggaacag caatactaaa ctggctaaaa
     9061 aaatccgaac attctactat tccggatttc gtaataccgg tatcgattat ttttatttta
     9121 tcttatttaa ttctttttgg tttatatatt gcaggtatac ttggttcgat ttggtttgca
     9181 gtttttctaa tgtacctgat cttttatgga aaatatcaaa agcctgcatc ttctgtgttt
     9241 gaagagtcag ctcttctctg cgatcatata agaaaattct cagtgctgat gcagcttgca
     9301 ggaaagtata atatgaaaaa tttcccggag acagaaaagt ttttagatat attcaaagcg
     9361 gaaaaagatg gtgctagtga tgagctgata aaacttgata aacttcttat gctaatacgt
     9421 ctaagggata atccgattta cagagtgatt ttcaatgcat ttattcctta tgattttatt
     9481 gtctgcaaaa aacttctgac acttatctca gatatagaac ataagcttcc ggagtggctg
     9541 gacaagtttt atgaacttga gtgtctgatc tccgtatcta atttttcata tttaaatcct
     9601 gattataatt atcctgagtt tgaaaccgaa agagagttta cttttgagat taaagaatgc
     9661 ggacatccgc tgctgcagag ggatcagaaa atttgcaatg attttaattt ttcaaaggaa
     9721 agtgaaacag taattattac tggatcgaat atgtccggta aaagtacttt tctcaaaact
     9781 gtaggcataa atcaatgtct ggcaaatgca ggtgctccgg taaatgcaat tagttttaaa
     9841 acttcagttt atgatctatt tacgtgcata aaagtcaatg attctgtggc tgatggtatc
     9901 tcatattttt atgctgaagt cagaagactt aaagaattaa ttgatgaaat aaagatttca
     9961 aaaaataaaa taaaattttt tctgatagat gaaattttta aagggacaaa taataaagaa
    10021 agactacacg ggagcaggga attcataaga tatttgtcaa aacaaaactg tgcaggcttt
    10081 ataactactc acgatctgga gcttgtaaac atttcagacg agattgatac tgttttaaat
    10141 tttcatttca gggaagaaat ccttaacaat gaaatgatct ttgattataa aattcataat
    10201 ggaccatgcc cgactacaaa tgctcttaag atcatggaaa ttgccggact gcctgtgtga
    10261 tcaggacaga tacaaaaatc atatttaata ttaaaggaat aaatactaaa ttgcattaag
    10321 ttcaaaaaat aaaaacttaa aaaaattatt cagataatta ctattaataa ttatattgtt
    10381 taatataaaa aaaaaattaa aatctaatgt ccggtgtaca ctaccggaaa attatatcaa
    10441 aaacaaatca ctaaagttaa ccttctatga ttatgaaccc ggaaataaaa gaagaaataa
    10501 agaaaataac agataagata gtagagataa aaaaaaataa aggtgacagt aattctgaac
    10561 ttattgccac agagaattct acaaaattta aggaacttat cactaatcat attatccctc
    10621 ttgttaaaga aagtaatatg ataataggca gatcaggttg ctcaatgaat tattattcaa
    10681 atgaactcgc tgttattaga gatccattaa aaagaatttt tattcagata ttattttatc
    10741 catatggtca cagtaagatc acacttggcg tgaatgctcc cttccttcag tttgcatata
    10801 gtccattaaa agaaaatata aagataacgc aaaagctttc tataaaatct gacgagccga
    10861 gaattaaagt tgatgagatc gagatcaatg atatagcaat ggataagatc aatcagtact
    10921 ttctggattt tctaaatgag actatagttt ataataaata acttttatta atcggtgaaa
    10981 ttttttatcc gatgattaca gttatgctat gtactaattt ttataacaat caaaactttt
    11041 caatgaaaat tttcaatcac actttttttt atttcctaat tttactttta ttacctttgc
    11101 acagtatatt tgcaaacggt aaatacattc ccacttcaaa aaattctgat gtaaaaaatg
    11161 aagtcaggct tatcagttca gataataata atactgtaat ttctgtaaga gttgcaggat
    11221 attctatagt ggaaagatca gtgaccggtg aaaattttga tgctataaac attgaaggtt
    11281 atacctcact tggcaatacc ggtcaggctt ctcttcctgt catgacaaag atgataagca
    11341 ttccaaattt taaaaatgta agtataaaaa tattaaattc atctgaaaag aattacagtg
    11401 taaataaaat ttacccaagt caggaatttc cgcttcgaaa ttcaaatgca gaagtaaaag
    11461 atttttcata tgataaaaat tattatacat ccgacaaaaa ttacccatct gaaattgtca
    11521 gcataaaaga gattgcagta atgcgtgatc acagagtcgc ggtcatttca ataaatcctg
    11581 taagatataa tcctgctaaa aatgaaatga cagtttatga tgaaattgaa tttgaattag
    11641 tttatgacgg atattctaat gtgaacaatg tcactaactc tgtttacagc agatcaaaga
    11701 gttttgaaaa tatttataag aatacgatct ttaattacgg tagtataaat aatgataaca
    11761 cttttcttat agctcctaaa atgcttattc tgactgctga ttcgttgtat aacagcattt
    11821 taccgtttgc tgaatggaaa aatcaaaagg gaattaaaac aacagttaca ttacgttcgg
    11881 aaattaacgc aaacggaaat ccaacttcga ctgaaattaa aaattatctg actgcaaatt
    11941 ataattctgc agacagaact gaatatgtat taattgtcgg agatgcaaga ggacgaaata
    12001 cactcacttg gtttaatgcg accggtggaa aatctgatca tccatatcaa tgtcttgacg
    12061 gaacagacat tctcccggac atagtcgtag gcaggatctc tgtacaatct gtttcagagc
    12121 ttgactcagc attggcaagt ttaatgcagt atgaaaaatt tcctgaaatg gatgatacag
    12181 actggtataa aagagctttg gtacttcata gtaatgacgg tatagatccg ataaatggtc
    12241 aggttgccaa gagtgttttt ctgaatgaag gtggatttac aaatgttgat attgcaatgg
    12301 aaagcacttc acaaaatcag atcacaagtt ttattaacgg tggagtgagc tggacctggt
    12361 tcattggtca tgggaatgaa acttcttggg caagtcctgt ttggaatatg tctaatatgg
    12421 taaacctaaa ttacgaaaat agaaaaccta caataatttc aattgcctgt tcaaatgcag
    12481 atctggatta ctcagaaacg aatgattgct ttggtgaagc ctggatagaa aggagtacga
    12541 aaaatactgc tataaacatt gcggcttcaa ctgagctatg ttccttttat acttcagata
    12601 ctattggaag agaaatgctt tacgggtatt tcagacacgg catctatgat tttggttcga
    12661 tgctgaactt tggaaaaatt caggcatata attatttcaa tggaagcggt tcagtaatag
    12721 ctactataaa tcaattcatg gttctcggag atccttcaca ggaagcattt tctgacgttc
    12781 caaaaaatat ttcccttctt cgtgattaca gcgggggtca acataaattt aatataaaga
    12841 ccggcagtga aaattataat ggtgcgttga ttgcaatcag tcaaaatagt gaactcaaat
    12901 cttccggtta cactgacagt ctcggaaatt attcttttaa ttccgatttg ataaataaca
    12961 acttgcctgt gaatatagta gtcacaggaa aaaatctgaa tccgtatttt ggaaatatga
    13021 tacttacttc aatttcttca ggtgtggaaa atccaaataa attttctctt aatcaaaatt
    13081 atcctaatcc gttcaaccca tatacaataa tttcatttag tattggtaac aatttaaatc
    13141 tttcggaaaa tgttatatta aaggtttatg atatactggg taatgaaact gcaacacttg
    13201 taaatgaaag gcttacttcg ggcagttatt ctatagaatg gaatgcaggt aatttttcaa
    13261 gtggtgttta tttctataaa ttgagtgcag gtaatttttc tgaggtgaag aaaatgacac
    13321 tgctgaaata atttaaataa tatatttcta atattaaata gctgatagtt tagaaaaatc
    13381 tgatttaaat aaagtttatt gaaaagctgt gagagatttt ctccacagct ttatttttta
    13441 taaggatatc gtttttattt tttatccgct aaaacccatt tcccgggacg tccttcactg
    13501 ctgataaaat acagcagact gtaaaaaaca attatgctgt ccattattcc catgcctgtg
    13561 taagcacttc cccagggttc atttatgttt gagataaata aaaataccat aaaagatttt
    13621 tcaagaattg aaaaaagcat tgtgtaaaat ctgagccatg gataaaaagc agcagcaatt
    13681 aataatgcgc cgattccgaa taccataaat ccccagtgtc ttaaagccgg tgaacttgaa
    13741 gctgtgaatt caattccttc tgcaaagtcg ggaagtaaat ttatattgaa agcaaatacc
    13801 agaggcagac aggtggcaag tccgaaaatt atcattaacg gtttggaggt ttttctgatg
    13861 aagttgttaa gagaattcat ttgaaaattt aattttacaa aactttaaaa tttaatttag
    13921 aagatcattc aattacagat taactaaaat ttattttcag ttaatataaa aaatattgaa
    13981 cacagacata agtcgaatga atattatcaa aatttaaata tttgttacaa gtatttattt
    14041 atgcataata aggtgaggag aaaaataatt gaaaaaagtt acgaaagaat taaaaactac
    14101 gtttgaacta aatatcataa tccttatatt tgtagaataa ccaacgaaat tttaatacaa
    14161 ctttttaatg tcaaataata caaataacaa aggtaagata ttacaaatta tcggacctgt
    14221 gcttgatatt gattttagcg gcggatcttt gccggctata aataatgcag ttgagataaa
    14281 aagaaaaaat actgaaggag ttgatgaagt tcttattact gaagtacagc agcatcttgg
    14341 tgaatcaaga gtaagaactg tagcgatgga ttccactgat ggacttgtaa gaggaatgga
    14401 agcaattgat acaggaatcc caatctcaat accagtaggt gaagaagtac ttggaagact
    14461 tataaatgta accggcggaa ctatcgacgg aaagggacct ataaatacag atcttaaata
    14521 tcccattcac agaccagcgc caaagttcag cgagctttct acaaagaaag aaatgtttga
    14581 aactggtata aaagttatcg atcttctgga gccatataca aaaggcggaa agaccggatt
    14641 atttggtggc gcgggagtag gaaagacagt tattattcag gagcttattc ataatatcgc
    14701 aaagcatcat ggcggatatt ctgtgtttgc gggagtcggt gagagaacaa gagaaggtaa
    14761 cgatctttat cttgagatgg aagagtcagg ggtattgaaa cagactgcac ttgtattcgg
    14821 gcagatgaac gaacctccgg gagcaagaca gagagtggca ttgacaggat tagctattgc
    14881 agaatatttc agagatcagg gaaaagacgt tttattattt gttgataata ttttcagatt
    14941 tacacaggcc ggatcggaag tatcagctct tcttggaagg atgccttcgg cagtgggata
    15001 tcagccgaca cttgcatctg agatgggtgc cttacaggaa aggatcactt ctacaaagac
    15061 aggatccatt acttctattc aggcgatata tgtaccggca gacgattata cagatcctgc
    15121 accggctaca acatttgctc accttgatgc tacaactgag ctttcaagga aaatttcaga
    15181 acttggaatt taccctgcag tagatccgct tacttcgact tcaagaattc ttgatccatt
    15241 ggttgtaggt gaagatcatt ataatactgc acagggtgtg aaaaaaatat tacagactta
    15301 taaagatctg caggacatta ttaatatttt aggtattgat gaattatcag atgaagataa
    15361 actgacagta gcaagagcaa gaaagataca gaagttttta tcacagcctt tctttgtggc
    15421 agagcagttc acaaaccttc ccggaaagta tgtaaagctt gaagatacaa taaaaggttt
    15481 taaaggaatc atcaacggtg aatatgatga tcttcctgag aatgcatttt atctggttgg
    15541 aacgattgaa gaagctgtag aaaaaggtaa aaaactttcc gagtaatatc aggaagtaaa
    15601 agtaataaaa ttaaatttta gaaaaagaaa ttattttata gaaattggat cagaatttat
    15661 atctggaaat agtgagtccc gtgaagatgg tttacagtga agaagtggat tcggtgacta
    15721 taccggggac agacggaagt tttcaggtgc tgaaaaatca cgcagctttg atcgcaacat
    15781 tttcattagg taagatcaag ataaaaaaag gtcaggagac aattatttat tcgacaagcg
    15841 gcggaatatt tgaagtaaag aataacaaag caatagttct tgctgaatcc attgaaactg
    15901 ttgaagagat tgacatagac agagcaaaac ttgcaaagac gcgtgctgaa gaaattttag
    15961 ctatggcaga agcaaatgaa gatgaagtaa gagatgctaa agatgcatta caaagagcga
    16021 tcaacagatt aaagatagca gaaaaaggaa aataagttta agtaatacca ggagggaccg
    16081 tagctcagtg gtagagcatc tgccttttaa gcagagggtc ggtggttcga gaccacccgg
    16141 tctcacaatg taaaacagaa accgcagttg attattcgac tgcggttttt ttgttttgaa
    16201 ttcaaataca gagtaattca ttttctgtaa aatataaaca tactattatt aataaataat
    16261 atttatcttt ctacattcaa aaataaaaac acaagaagga gtaaaaggaa tgaaaaaatt
    16321 ttttttaatt ttgtgctttg tttatactgc atttcaaagt tcggcaaatg catctccacg
    16381 aatcagcttt gaagatcttt taattcagag tgaattaaac ccccaaaatg tattacaagc
    16441 aagagaaacc gccatcagac aaggtattcc tgtttctatt atgacgtccg acaggataat
    16501 gatcgatgca aaatatgtag aggatggaag accggtatat gccgtgtata caaatttcgc
    16561 tgatatatat aacggtggat atgccgctta ttatgaagat gtggtgtcaa aaattaattt
    16621 tgcaaatgca agaattgatt acggaaacag aaatattgtc gataatacaa acggtttttt
    16681 tactcccgta ttatccggca ggaatgcggc ttcatcattt attatgatta ctgaatccac
    16741 tagtgacaga gtatcaatat tcaatgccga aaccggcgat ctgatagata cagcttttat
    16801 tccttcgacc aggccgcaac ttggaacacc taaggtagct ctaaagcact atgcaaattc
    16861 aaatgttata atttgtgatc agataactga cgtcgttcag gattttaatc ttaacggtac
    16921 ttacaaaggt ttctatgcac catccaccgg ccctaatact tcaattgtcg ataatatgag
    16981 aggcatgcgg taccggtcaa ataacaatat agttgtttct gttggcagcg gtgtgaatca
    17041 aaatacaatt cagcaattcg atccatctgg aaatcacata ggaacatata ttacaggtaa
    17101 tttaaacagc ccgtttgata tattgataag agctaatgat gtcctggttt caaatttttc
    17161 cggaactaac agagtaagca gatatgattc aaacggagtt tttttaaatt cattttatac
    17221 aggttcgaat tttggaattt cacagcagat gtatgaagta gctgcgaatg gaaacgtcgc
    17281 tgtctcatca tttcaaacac cttccggatt agcaattctt aactctgccg gtgcttttgt
    17341 aaaacttctc aacggtgtaa caggaaacag aggagtacat ttattaggaa acggaaattt
    17401 cttagttaca aatagtacag gcgcacatga aatagattca gcttcaggca gtcttgtcag
    17461 aacaattcat cttagcccga atttccagta cgccacaaaa tacgaaatac cgaatccata
    17521 tcttagacta agaataaatc tcgaagcatg tgctattctt gatactgtca atgttgacat
    17581 acgaaattct tcttctcctt tcagtattat tgagtcaaaa aaagtggttg ctggattagg
    17641 aaattctgtt gatgttcagt ttaatacacc ggtaaacggt actccttatt atatacaggt
    17701 aagtcataga aattcgattg ctacctggag cggaagcact ccttctttta attccgatta
    17761 tattaattat acattcattt cggcaagctc gcaggcattt ggcagtaata tggtaaacgt
    17821 cggaggaaaa tggtctttct ataccggtga tgtagatcag gatggcgtag tggacgttac
    17881 cgatgtagga ctgattgaca atgattcatt caattttgcc agcggatatc tttcaacgga
    17941 tctggattgt aatgatataa ttgacattac tgaccttgct tatgctgata ataatgcttt
    18001 taattttgtg agtgaggtgg ctccctaatc catataaatt tttcttaaaa taaaagggat
    18061 cgtaaattta cgatcctttt tttttagaat attatttttt tttcaggaca atgtcacgtc
    18121 ctgaaaaaat taaaaaaatt aattgattgt aatgataagc atttccccta tacttggtgt
    18181 gtattagtgg gattaagcat cggtgaaaca atcaagatta aattaaaaaa catgaaagaa
    18241 aacaaaagag tattttcaga tgggcttttg ataggaagaa taatctacac attttgaaat
    18301 ttactcatta gccagtcctt atcggcaata tttgtctccg atttcagatc cattattata
    18361 ttctttaagt atgtcttttt agatgatact gtattattta aaaggatctg ataataattt
    18421 gtaaactgaa tttctcttga tccaatattt attgaaaggt tactactgtt attcaggtag
    18481 tgtttgtaat tagacagaaa agatctggct gagtcaaagt atccgagttc atacatgact
    18541 ttaagtgtga ttttgctaac cccgattttc aatgtaatat ttttcagact tgccagactg
    18601 cagttattaa gacactcttt aaattctcct ttatagaata aaaaatttaa ataaccgtat
    18661 cgtgagaggt ctgctgagtc agtctgatca gggttatttt tacaatttaa aataaaatca
    18721 taagcccagt caaactgtcc cactttcaaa
//
LOCUS       DAORVQ010000043        18685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1211_length_18685_cov_5.768384, whole genome shotgun sequence.
ACCESSION   DAORVQ010000043 DAORVQ010000000
VERSION     DAORVQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 18685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18685
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_18685_cov_5.768384"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..836)
                     /locus_tag="PLX80_05350"
     CDS             complement(102..836)
                     /locus_tag="PLX80_05350"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HQY20254.1"
                     /translation="MECKNCGNDFTGKFCNECGQKNVDGRITIREILHNFIHSFTHID
                     SGIFFLIKELFIRPGAVVREYLSGKRKKYFNPFQFLIIAIAIAAFLAVKFTLFGPNID
                     PDTIEALNSQQRFWLLFNKFIYRNFNLILFLSVPVAAVYSRLIFRKSGYNYAEHLVFN
                     TFLAAERTVLYIILTPLIYFTKSNWTIGVGIYYILWVIYYGYSYVSFFGGNKFITILK
                     YICVLLLLMLTSQTISMSVFYFFFYK"
     gene            complement(912..1613)
                     /locus_tag="PLX80_05355"
     CDS             complement(912..1613)
                     /locus_tag="PLX80_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HQY20255.1"
                     /translation="MKINTKKSLKSTLMMIICLVSFIKFSGCSEDDILIPVVTYSNLS
                     VYHVDMITGNLDIQINNVVAASNVVYLERLNYQQLLPGNNNLKIQNASGTTLIDTTFF
                     LSENNYYSVFIYDVGGVAKPLIVTDNLTNPGTTNSSVRFINFAPGAGSLALGGTSKPS
                     PWFPNEAYGVPASFRPITGGVYNLYMNEALTPNTLATLIDQTLAAGRIYTIIGKGIAG
                     GTGNQNIGIVVYNNQ"
     gene            complement(1648..2697)
                     /locus_tag="PLX80_05360"
     CDS             complement(1648..2697)
                     /locus_tag="PLX80_05360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20256.1"
                     /translation="MQKRILIVRMDKVGDVVMITPMIRELKKKFPDSFVGTLTSENTA
                     DILMNNPYLDVSITDDMKKGSFGKVVKEIRSHKFTDGLLVMPTERAAYQMFLAGIKNR
                     IGVGKKLYEVITFMKSVSRNNYIPLRHEADYCMDLARALGVVTDDLIPEIFLTEIELK
                     EGGEILSGLGCNMDSEKIIVHTGSGNSSRNWSEDKYMNLIKEIIKIKPNAYIILTAKE
                     MSGDFQDSIRKLNSEKIINAAVSIKRLRDLIKIIGNADVLIASSTGPLHIASALKVKT
                     IGLYCHRAMNCAKHWGALGNKAVNIEVRRENCDLYCSSDKEICEFENGITIKEVLKIM
                     AYNKIIYLIFTIYLY"
     gene            2973..5186
                     /locus_tag="PLX80_05365"
     CDS             2973..5186
                     /locus_tag="PLX80_05365"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20257.1"
                     /translation="MKKLFDLKLFELSNAVKISIMLQCIFFQSIQIYSQSQWQWQHPL
                     PTGNTLNSIYFVNDNTGFAAGSMGNIIKTTDKGINWISLNSGTQKEIFKMEFWNLDLG
                     FCVCKDGLILITNNGGVNWKQISNYSSFDLYDICIKENKIYVCGLGGKILMSEDHGNT
                     WVNLNSQINIPLFCVNFLNGDTGICGGYNEIIRTTNAGISWEFLKADIPFSSQIKEIE
                     YLSPNKIFAAGNSPQGDFYISNNGGVNWERNSLGLPYLFGGSVDLVRSMDFADPNHGC
                     IVTDLGTVLITSTGGKQWLKDTSQRSGFLKEGIFKNVFFKNSSELFLCGNGGRVLKFS
                     DKGNNWNVLSGNNLNLYDSYVTDNGTVFCAGDNGILFRKKISNSVSNLKSEWEKILIR
                     DNLILSSVCFVNDFTGFVCSYNGLIFKTTDGGDNWKEVITFVKQNPDFHLNKIFFKDK
                     YNGFICGGNEYNSTGTILKTTNSGDSWKSLFSNFTIGTFKDIHFTDRENGFVVGDNGN
                     LTVTSDGGNTWSTQNLIPYDLNSIDFNGGISGLIVGENGTVFRTSDGGAKWDYISHIQ
                     YKDLFSVKFTDSFNVTAVGKNGTVIHSSDAGISWVKMNSLTDNKLLTLNCNSAGVFAF
                     GENGTIIYSESEVKVFSTLSNAAPKKNISSLSNYPNPFNPHTNINYIVGYTNNVSIKV
                     YDILGNELALLVNEMQVSGNYDVKFDGSNYASGIYFYAISFNGKLHETKRMVLKK"
     gene            complement(5226..6602)
                     /locus_tag="PLX80_05370"
     CDS             complement(5226..6602)
                     /locus_tag="PLX80_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20258.1"
                     /translation="MIIITCISITGIKAQSDSALKSADKISYDLFNKAEWNSLISFSE
                     NALKKNIDFIFLRVRLGIAYYETGKYFDAVKNLQEAFDKGYETPLLIEYLYFSYLEAG
                     RKEDADYYFYKLPENKRKRLKPQDNSFMYEIHVDAGYGLSNDENKNENIDLDGSFNAY
                     GEQTLFGNYFYLNAGFKQLPLRWLNVIYSYNYLSLKKTKQIMYNNVKTSDPYTQQQQQ
                     FYIKYDIRAGDGFVISPSGHYIYTSDNTIYSKFDSVNYSYNMQTMTYDSLNYFYTINN
                     KPAIQDNFVLSLSLSKQMYIFKAALHGSFSYLNGNHQSQIGANFKAYPKKKNTLYTFT
                     DLTLQNQNDVTNLIVYQSLGGKLHKNLWGEAFVTIGKMNNYNEQNGAVVYNNPDVINL
                     KFGGEMKYYFNENLSASLIYVNEQREKNYLTYTLTKNGNNSPIANPEYLSLDYSVNTI
                     LAGLKLYF"
     gene            6837..7589
                     /locus_tag="PLX80_05375"
     CDS             6837..7589
                     /locus_tag="PLX80_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HQY20259.1"
                     /translation="MDYPKRVVVKGEKDKKIVKEVQKKLNENGCGPIDVDGDFGPKTF
                     NAVKLFQSRFTDQEGRPLIIDGKIGPLSWITLFKLKAIGPEPGPLSDFTKKVLSIAKG
                     QIGVIEKPRGSNSGPEVDAYLKSVGLGPGYAWCAAFTYWCYNEAYKNSGKSNPAYKTA
                     GVLSHWTNTKGKKITSSAAFANPSLVKPGQVFIMSYGGGLGHTGIVESVNGGYLTTIE
                     GNTNDGGSREGIGVFKRTSRKINSINKGFIQY"
     gene            complement(7705..8199)
                     /locus_tag="PLX80_05380"
     CDS             complement(7705..8199)
                     /locus_tag="PLX80_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20260.1"
                     /translation="MKKFSFILNNFYSIFNPMPQGNNKNIDSYLDDSIRHSLRSETSE
                     DFMYEMMKRVEIEKEFAREDRKTDKTVKFVIGGLVAVMILIAFGLPFFFGTSDADSSY
                     GYYNIMIDQIAGSIEYFSIFMTDNLGIAVNSKTVLVLLISGLCIFLFSKADKLFLKKS
                     LNQN"
     gene            complement(8211..8858)
                     /locus_tag="PLX80_05385"
     CDS             complement(8211..8858)
                     /locus_tag="PLX80_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY20261.1"
                     /translation="MKLSDSEIIYNIKKGDTSSFRIIAEELKDKAFSLTMKILRNKEE
                     AEDCLQESFMKLFKSITGSKFEERSKLSTYFYTIVYNTAVDNYKKLKSRRFSILSIDV
                     EGSSFEEGDDLVKNYYDTKINKSEFDDKYDLSTESKISETEIKKIINDYINSIPEQYS
                     VILTMFYINDLSHDEIAGILKLPLGTVKNRIFRAKNKLKEIILKKYSEEEILQYV"
     gene            complement(8925..9725)
                     /locus_tag="PLX80_05390"
     CDS             complement(8925..9725)
                     /locus_tag="PLX80_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20262.1"
                     /translation="MKTSIIVIAVIFGFLFNSCGLLSKKYTKSAKEIHAVLSEGKTKL
                     VLNNINGNVNIKQSDDSGFVKIKAEKEIKVKKKHLDTPFDEITIEIDTIGSVINIETV
                     IGKRGEDDGFNLNFDRRQKVDYEIFVPAGISIIIENVNGTVTAEKISNDLVIHQINGD
                     VEIDKYSGQLECEITNGSLSGKIDSTKGMNINIINGKVSLGLSNYMNAKVNAESLNSK
                     VTTENLSFTVMDQENKSFKGSLGSGEEKSIISIETVNGKIFLFGEQDI"
     gene            complement(9751..10134)
                     /locus_tag="PLX80_05395"
     CDS             complement(9751..10134)
                     /locus_tag="PLX80_05395"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HQY20263.1"
                     /translation="MNNALEAFIPVVAIVFTFGIPGVLLFWWIYTKHREKMRLIEKGL
                     SPEEVKAYFADNTKPKNPYASLKFGLVLLFLGFGIFLANVAESIYDVEEGVMMGIIVT
                     FIGLGFLIYFLVVNSKNKNETTLNQ"
     gene            complement(10521..11048)
                     /locus_tag="PLX80_05400"
     CDS             complement(10521..11048)
                     /locus_tag="PLX80_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HQY20264.1"
                     /translation="MLEAVQKKWWVLLIRGILLLIFGILALATPGMILATMLFYFGFI
                     AIFSGIFVIIEGFMSSEDKGTIILEGIFYILFGLLFLIMPGFVISFTLYFIAFWALIA
                     GIFMIVKAIKLRKVIQNEWLIIFNGIITVILGILIFANVFAGAEILVMVFGFYAILSG
                     IMMMMISFKVKGLKK"
     gene            complement(11109..11432)
                     /locus_tag="PLX80_05405"
     CDS             complement(11109..11432)
                     /locus_tag="PLX80_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HQY20265.1"
                     /translation="MGIIVIACFKPKEGKEKELLEVLKDHMPVLRKEGLITDKECIVM
                     KSENGSILEVFEWKSQDAINHAHENSNVLDLWKRFEDACTYETLSTLDESAKMFPGFD
                     PVKFN"
     gene            complement(11475..11894)
                     /locus_tag="PLX80_05410"
     CDS             complement(11475..11894)
                     /locus_tag="PLX80_05410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY20266.1"
                     /translation="MNSTDLLNSSKAPEPVGLYPHARRFGDLLFLSGVGPRERGTKKI
                     PGVELDNSGNIISYDIETQCRSVFQNVKYILEDAGSDWSKLIDVTVFLTNMKDDFATY
                     NRIYAEYFKVNQPCRTTVEINSLPTPIAIELKCIAYI"
     gene            12044..12679
                     /locus_tag="PLX80_05415"
     CDS             12044..12679
                     /locus_tag="PLX80_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQY20267.1"
                     /translation="MKILSVNTGGPQKFMWEGKEITTSIFKSPAEGKFETTFLNINGD
                     IQSDLKNHGGLDKAVYSYDHSYYETWKKEMDRESWEYGLFGENLTTSGLTDDSVLIGN
                     VYQIGSVKLRAVQPRFPCFKLNIRFGEDHILNRFYDLGFFGTYFRVVEEGYLNTGDEI
                     ILIHKSDINLSIADIVFCKKSKGEDQYKLNLILENQYIPDSLKDSLRIYLK"
     gene            complement(12680..14023)
                     /locus_tag="PLX80_05420"
     CDS             complement(12680..14023)
                     /locus_tag="PLX80_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY20268.1"
                     /translation="MRKYIIVKIIILLTISALVQVRGISQTSQSNDVVLSNEMKMNTV
                     NKVSQMLNDNYIFQDKASEMGTLIVNNFESGKYSDLNDPAKFALQLTEDLQSVSKDKH
                     IRVRFSPDQIKMMKQSEGQDENSDQTYLEEMKFDNFGFRKVERISGNIGYIDFRQFGS
                     GDQIKDKVASVMAFVENCDALIFDMRNNGGGDPTGIQVITSYLFGEEPVHLNDLYYRP
                     KDTTEEFWTLKNIEGKRKPEVPVYVLTSSYTFSGAEEFSYNLQNLKRATIVGETTGGG
                     AHPGGMNIIDDNFAIFIPTGRAINPITKTNWEGTGVKPDVEINSINALTKAHILALEK
                     ISADLTDENMKRKYTWMVESLTTTLNDVEIDAALLKKYEGEYGDRKIYLENGKLFYQR
                     SGRQKFELTPMSENTFMLSEIDYFRVRFESDGNGNITSVTGLYDDGNTDSSPKSN"
     gene            complement(14156..15133)
                     /locus_tag="PLX80_05425"
     CDS             complement(14156..15133)
                     /locus_tag="PLX80_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HQY20269.1"
                     /translation="MNTIKQLGYIAIGTRLRMLTDKFISDADKIYKDTGLDFEPRWFT
                     VFYLINLKKSITVTEIADQLGYTQPAVTQIANSLVKKKLISAVKEKKDSRKKILTITA
                     KGEDLLLELIPVWQDIENSVKELFSDTGYDVLFVISKLESELEKNDIYSRVMEKSKKR
                     QLEEIKILEYDNSKKNEHKKYFKELNYEWLNKYFKVEKEDEKQLENPEKEIINKGGYI
                     FFAEYKDEIAGTAAVLKREDKTYELAKMAVKNEYQGKQIGKKLGNAVIEKVKQLKCDN
                     LYLDTSSLLTPAINLYKNLGFEEVEMKDPSKYERDTFRMSYNFKKINQK"
     gene            15307..16761
                     /locus_tag="PLX80_05430"
     CDS             15307..16761
                     /locus_tag="PLX80_05430"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HQY20270.1"
                     /translation="MNLRSKGVRSFQLYQNKLFKTTSILFLLLTIIPFLIISFFTNPT
                     WDDFDFTVNALKFGFLRSQYNWYIGWSGRFFSTAVMSSVNPLMFHSIIGYKLASFSVI
                     ILFIISVFYFIKYVTGNTLNLTQYSIAALSICSLYFYKMANPALGFYWLSSAVIYQLA
                     NILFILLIILLIKYSGQNSKLIRSVYLIIISFVIIAIQGSNEISMLLTMEFLGFLFIY
                     HLFKFKKINYSLLFFIVISIIGFYLVYTAPGNGVRESKNPQSHMLFSSLSLSLSDTAE
                     LILYRVTDTPLILFSILVLPFILKISENHSDTKRYFDCNPVISVFSVFVILLSINFVT
                     YWTINKGMLDRTRNVFYFFFLIGWFYNLLVFCNYFIKYRSVKNFKLPALAVIIISLLI
                     VISYGRKNNVLLAYKELSNGSLINFDHELSDRYDYIRKENSDTCILKPLSNPIPGLMF
                     FYDVGADPNAKHNKYQAYYYHKKFMLVNDTSKKE"
     gene            16825..>18685
                     /locus_tag="PLX80_05435"
     CDS             16825..>18685
                     /locus_tag="PLX80_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY20271.1"
                     /translation="MLRSYLIILSIFLYVSVCYSQNEIYILPGENLITDGLPDIPVSL
                     SVELEKYTESKSAVLSDWHPVNKEMIMSTRIGNTSQLKYLSSPLDTGMQLTFFDEPVR
                     SASFDPVNGDFFLYYKDTGGDEFRQIYRYDLKDGNTTMITKGGREQNSGIEWLGSGNH
                     VAYTSTRRNGADRDIYIMDPKNPDSDSLFLELKGGGWGILDCSEDGKSLLLMEYISNS
                     ISYYWLADLTTGKLSELTGKSEEDIYYSQAMFSKDGNGLYFITDKDHEFKTLAFMDLK
                     NKSAKFISDNQTGDVESFNLSDDGQSLAYIVNEYGESKLYVYNTVTGISSQINNLPVG
                     VYGSLLFHNNSEDLALTISSARSSRDIYVYNLKSDALTAWTESDMGGIIPEELSIPEL
                     IKWNSFDGLEISGFYYKPHPKFSGKRPVIIDIHGGPASQSRPSYLGSYNYYLNELGIA
                     IILPNVRGSAGFGKTFLKLDNGFDRKNSVKDIGALFDKIELIPGLDADRVMVMGGSYG
                     GYMSLAISVDYADKIRGAVDVVGISNFNTFLKNTESYRTDLRRVEYGDERDPAMYEFL
                     EKISPLNNAEKITKPLLIVQGGNDPRVPRTEAEQMMKTIKDLNGTVWYLEAKDE"
BASE COUNT         6006 a         3162 c         2928 g         6589 t
ORIGIN      
        1 atcattatta attttgaaga aatagaggta tgccaaatta attaaatatg aagtttaaat
       61 caattaataa gaagataaat tttgtaaaaa actaaatccg attacttgta aaagaaaaaa
      121 taaaacaccg acattgaaat ggtctgactt gtcagcatca gcaataaaag cacacagata
      181 tattttaaaa tagttataaa tttattcccg ccaaaaaaac tgacatatga atatccgtaa
      241 taaattaccc ataatatata ataaattcct acacctatag tccagttcga tttcgtgaag
      301 taaatcagcg gagttaagat tatatacaaa actgttcttt cagccgctaa aaaagtattg
      361 aatacaagat gctctgcata gttataacct gacttcctga atattaatct ggaataaact
      421 gcagctaccg gcacgctgag aaacaatatt aagttaaaat tcctgtaaat aaatttattg
      481 aacagtaacc agaatctctg ctgggaattt aacgcttcta ttgtatcagg atctatgttt
      541 ggaccgaata aagtgaattt tactgcaaga aaagcagcga ttgcaattgc gatgatcaga
      601 aactggaatg ggttgaaata ttttttcctt ttaccgctga gatattctct gactactgcc
      661 ccggggcgga taaataattc ttttatcaga aaaaaaatac cggagtcaat atgagtaaaa
      721 gaatgtataa aattatgaag tatctcccgg atcgtaattc tgccgtcaac attcttctga
      781 ccgcattcat tacagaattt accggtaaaa tcattgccgc agtttttgca ttccattttc
      841 tttcacaaag aaattacatt gtaaaaaaaa agcccgcatt tagcggactt ttttttatga
      901 tttattttat attattgatt gttgtaaacg actattccta tattctgatt acctgtacca
      961 cctgcaatac ctttacctat aatagtatag atccttcccg ctgccaaggt ctgatcgata
     1021 agagtagcta atgtattcgg tgtaagagct tcattcatat atagattata aacaccgcca
     1081 gtgatgggtc tgaaacttgc aggaactcca taagcttcat ttggaaacca tggagaaggt
     1141 ttacttgtac cacctaatgc aagagatccg gcgccgggtg caaaatttat aaatctgaca
     1201 gatgaatttg tagtacccgg attagtaaga ttgtcagtta caataagagg ttttgcaaca
     1261 cctccgacat cataaataaa aacagaatag taattatttt cagaaaggaa aaatgttgta
     1321 tctattaatg ttgtaccgga agcgttttga attttcagat tgttatttcc cggtaagagt
     1381 tgctgataat ttagtctttc aagatagact acatttgatg ctgccactac attgtttatc
     1441 tggatatcta aatttccagt aatcatatcc acatgataaa cgctcaaatt tgaatatgta
     1501 actacaggaa taagtatatc gtcctccgaa catccggaaa acttaataaa tgataccaaa
     1561 caaatgatca tcattaaagt tgattttaga gatttttttg tatttatttt cattattttt
     1621 ttttaatttg aaaaaaattg tttacagtta atataaatat atcgtaaata taagataaat
     1681 aattttatta tatgccatta tttttaatac ttctttaatt gtaattccgt tctcaaattc
     1741 acaaatttct ttatcgctgc tacagtacaa atcacaattc tcacgtctta cttcaatatt
     1801 cactgctttg ttccctaaag ctccccagtg ttttgcacaa ttcatcgctc tgtgacaata
     1861 taaccctatc gtttttactt taagagcaga tgcaatatgc agcggacctg tggaagaagc
     1921 gattaaaaca tctgcattac caattatttt aataagatcc ctgagccgtt taatagacac
     1981 agcagcattt attattttct cactatttaa ttttctaata ctgtcttgaa aatcaccgga
     2041 catttctttt gctgtaagaa taatatatgc gtttggttta attttaataa tttcctttat
     2101 caaattcatg tatttatctt cactccagtt acgtgaggaa tttcctgagc ctgtatgaac
     2161 aatgatcttt tcagaatcca tattgcaccc cagtccggaa agtatttcgc ctccctcttt
     2221 caattctatt tcagttagaa aaatctcggg aatcaggtca tcagtgacaa ctccaagcgc
     2281 acgtgcaaga tccatacaat aatccgcttc atgtctgagc ggaatgtaat tattgcggga
     2341 gacacttttc ataaaagtaa tgacctcgta gagtttttta ccgactccga tgcggttttt
     2401 tattcccgcc agaaacattt gatatgcagc tctttcggtt ggcatgacaa gtaaaccgtc
     2461 tgtgaacttg tgagatctta tctccttcac gacttttcca aatgatcctt tcttcatgtc
     2521 atcagtaatg cttacatcga gatagggatt attcattaat atatctgctg tgttttcaga
     2581 tgtaagagtt cctacaaatg aatccggaaa cttttttttc agctccctga tcattggtgt
     2641 tatcatcacc acatcaccga ccttatccat ccgcacaatg agaattcttt tttgcattcg
     2701 ataaattaat caatcttaca tggaatatat aaataaaaat tcagtaggtc ttttaataat
     2761 tttaaatcta attatgaaat tataaattta taacacctgc cgattttaaa tcaaagttta
     2821 ctaatctaaa tcgttcacgc aaaatcactt gcttatcgga ttcttcggat catgaattaa
     2881 attcattttc acaaacacag aatattacca attgactttt ctgtactgtc tgaatatttt
     2941 acacaggaat taattaaaag gagaagatca cgatgaaaaa attatttgat ttaaagttgt
     3001 ttgagttatc taatgctgta aaaatttcca ttatgctgca atgtattttt tttcaatcaa
     3061 tacaaattta ttcccagtct caatggcagt ggcagcaccc tcttccgaca ggcaacactc
     3121 ttaacagtat ttattttgtt aatgacaata caggatttgc agccggctct atgggaaata
     3181 taattaaaac taccgacaaa ggcataaact ggattagtct gaattcgggg acacaaaagg
     3241 aaatttttaa aatggaattc tggaatttag atttaggctt ttgtgtctgc aaagatgggc
     3301 ttatactaat tacaaataac ggcggggtca actggaagca gatcagcaat tattcctcat
     3361 ttgatctgta cgacatttgt ataaaagaaa acaagatata tgtttgcgga ctcggaggaa
     3421 aaattttaat gagtgaagat catggcaata catgggtaaa tttaaattca caaatcaaca
     3481 ttcctctgtt ttgtgtaaat tttctgaacg gggacaccgg tatttgcgga ggttacaatg
     3541 aaattatccg gactaccaat gcaggaatca gttgggaatt tctcaaagct gacattccgt
     3601 tttcttcgca gatcaaggaa attgaatatt tatcccccaa taaaatattt gctgctggta
     3661 attcaccaca aggcgacttt tatatttcaa ataacggagg tgtcaattgg gaaagaaatt
     3721 ctctcggact tccctacctt tttggtggaa gtgtcgatct tgtcaggtca atggattttg
     3781 ctgacccaaa ccacggctgt atcgttaccg atttgggtac tgtactcatc acatcaaccg
     3841 gtggaaagca atggctgaaa gatacctcac agagatccgg atttctgaaa gaagggattt
     3901 ttaaaaacgt attttttaaa aacagttctg aattattttt atgtggaaat ggaggaaggg
     3961 ttttgaaatt ttcggataaa ggaaataact ggaatgtact ttccggtaat aacttgaatc
     4021 tctacgacag ctatgtcact gataacggaa ctgtattttg tgcaggagat aacggaattt
     4081 tattcagaaa aaagatctca aattctgttt ctaatttaaa gtccgaatgg gaaaaaattt
     4141 tgatcagaga taacttaata ctcagctcag tttgttttgt aaatgatttt acaggatttg
     4201 tttgcagcta caacggcttg atcttcaaaa caacagacgg aggtgataac tggaaagaag
     4261 tgatcacctt tgttaaacaa aatcctgatt tccatttgaa taaaattttt tttaaagata
     4321 aatataatgg attcatctgc ggtggtaatg aatataattc aacgggtact attttaaaaa
     4381 ctactaacag tggtgattca tggaaaagtc ttttcagtaa ttttactatc ggtactttca
     4441 aggatataca ttttactgac cgtgaaaatg gatttgtagt tggcgacaat ggaaatttaa
     4501 ctgttacatc tgatggagga aatacatgga gcacacaaaa tttaattcca tatgatctta
     4561 actcgataga ttttaacgga ggtatatcag gattgatagt tggtgaaaac ggaactgttt
     4621 tcagaacttc agacggaggt gcaaaatggg attatatatc tcacatccaa tataaagatc
     4681 ttttttccgt aaagttcact gatagtttta atgtaactgc tgttggtaag aatggaacgg
     4741 tcattcattc ttcagatgca ggaattagct gggtgaaaat gaattcatta acagataaca
     4801 aattacttac cctgaattgt aatagtgcag gtgtatttgc tttcggtgaa aacggtacta
     4861 ttatttattc tgaatctgaa gttaaagtct tcagcacttt atcaaatgca gctccgaaaa
     4921 aaaatatttc atcactttca aattatccaa atccatttaa cccgcataca aatataaatt
     4981 acatagtagg gtacaccaat aatgtttcaa taaaagtgta tgatattttg ggtaatgaac
     5041 ttgcattatt agtgaatgaa atgcaggtat caggaaatta tgatgttaaa tttgacggca
     5101 gtaattatgc gagtggaata tatttctatg cgatttcttt caatggaaag ctgcatgaaa
     5161 ccaaacgtat ggttctgaaa aaatgaaatg agctgaaagt aaattaactt tctaaaattc
     5221 aatttttaaa aatacaactt cagtcctgcg agaattgtgt taactgaata atccagactt
     5281 aaatattcag gatttgcaat aggactattg tttccatttt tggttaaagt atatgtcaga
     5341 taattttttt ctctctgctc atttacatag atcaatgatg cagaaagatt ttcattaaaa
     5401 taatatttca tttcaccacc gaatttcaaa ttgatcacat cgggattatt ataaactaca
     5461 gcaccgttct gctcgttgta gttattcatt ttcccgatag ttacaaatgc ttcaccccag
     5521 aggttcttat gaagttttcc gccaagtgac tgataaacta ttaaattagt aacatcattc
     5581 tgattttgca aagtcagatc agtaaaggta taaagagtat ttttcttttt aggataagct
     5641 ttgaagtttg caccgatctg actttgatga ttaccattca gatatgagaa actgccatgc
     5701 agagctgctt tgaaaatgta catttgtttt gaaagagaaa gtgacaacac aaaattatcc
     5761 tgaattgctg gtttattatt tattgtataa aaataattaa gtgaatcata tgtcattgtc
     5821 tgcatattat aggaataatt cacgctgtca aattttgaat atatcgtatt atcacttgtg
     5881 tagatataat gtcctgacgg actgatcaca aatccatcac ccgctctaat atcatacttt
     5941 atataaaatt gctgctgctg ctgagtatat ggatctgatg ttttaacatt gttatacatt
     6001 atctgttttg tttttttcag gctgagataa ttataactat aaataacatt aagccatctc
     6061 agaggtaatt gtttaaatcc ggcattcaga taaaaataat taccgaaaag tgtctgttca
     6121 ccataggcat taaatgatcc gtccagatct atattttcat ttttattctc atcattacta
     6181 agtccgtatc cggcatccac atgaatttca tacatgaaag aattgtcctg aggtttcaat
     6241 ctctttcttt tattttcagg aagcttatag aaataataat ctgcgtcttc ttttctgcct
     6301 gcttccaaat atgaaaaata aagatattcg ataagcagag gagtctcata acccttgtca
     6361 aatgcttctt gaagattttt tacagcatca aaatatttac cggtttcata ataggcaatc
     6421 ccaagtctca ctcttaaaaa aatgaaatca atatttttct ttagagcatt ttctgaaaaa
     6481 gaaataagac tattccattc agctttattg aacagatcat aagaaatttt atctgcgctt
     6541 tttaaagctg aatcactctg agctttaata cctgtaatag aaatacaggt aataataatc
     6601 atcaggaaaa agattctgct gatatttgtt ttttgtagtc tgatataaaa aatttgtttg
     6661 taatttgtca ttatttagaa ctaattcaaa gtctaaatta ctaatcactt ttattttgaa
     6721 aattacacat tgtacatcat tcgtttataa tggaaaatta agtatgtccg cttattgaaa
     6781 tttctataca gaatatatat tttgatttaa tattttttaa aactaaataa ataattatgg
     6841 attatccaaa aagagtggtc gtcaaaggag agaaagataa aaaaattgta aaagaggtac
     6901 agaaaaaact taatgaaaac ggctgcggtc ctattgacgt ggatggagat tttggtccga
     6961 aaacttttaa tgccgttaag ctttttcaaa gtagatttac cgatcaggaa ggaagaccat
     7021 tgataataga cggaaagatt ggtcctcttt catggatcac tctttttaaa cttaaagcta
     7081 tcggacctga gccgggtccg ttgtcagact ttaccaaaaa agttctgtct attgcaaaag
     7141 gtcagatcgg agttatagaa aaaccaagag gaagtaacag cggaccggaa gtagatgcat
     7201 atctaaagag tgtcggctta ggtccgggtt atgcatggtg tgctgcattt acatattggt
     7261 gctataatga agcttataaa aattcaggta aaagtaatcc tgcttacaaa accgccgggg
     7321 tattaagtca ctggactaat actaaaggaa aaaaaataac ttcaagtgct gcatttgcaa
     7381 atccctcact cgtaaaacct ggtcaggttt tcataatgtc ttatggcggt ggattaggtc
     7441 atacgggaat agtcgaatct gtgaacggag gctatcttac tacgatcgaa ggtaacacaa
     7501 atgacggagg cagcagggaa ggaattggtg tattcaaaag aacttcaaga aagatcaaca
     7561 gcattaataa agggtttatt caatattaaa tattaaattt taaatattaa attttaaatg
     7621 gttagtagtt tgcagttaat aattgaatgt tgattttaag attcaaattg aaataatata
     7681 tattaaatat ttaaaattta ttattcaatt ttggttcagg ctttttttga gaaaaagttt
     7741 gtctgctttt gaaaaaagaa atatgcataa acctgatatc agaagtacca gcactgtctt
     7801 ggaattaact gctatgccta aattatcagt catgaatata ctgaagtatt ctatagaacc
     7861 tgctatctga tctatcatta tattgtaata tccataactg ctatcagcat cggaagtacc
     7921 aaaaaagaac ggaagtccga atgcgatcaa tatcatcaca gctactaatc cgccaattac
     7981 aaactttact gtcttgtcag ttttcctgtc ttctctggca aattcttttt cgatctctac
     8041 cctcttcatc atttcataca tgaaatcttc agaagtttca gatcttaagg aatgacgaat
     8101 actgtcgtcc agataagagt ctatattttt attattacct tgaggcattg ggttaaagat
     8161 actgtaaaaa ttatttagaa taaaactaaa ttttttcata tttttcgaat ttaaacatac
     8221 tgaagaatct cttcttcaga atactttttt aatattattt ctttgagttt atttttagct
     8281 cggaatattc tgttcttaac tgttcccagc gggagcttta gaatacctgc tatttcatca
     8341 tgtgaaagat cgtttatata aaacatcgta agaattacag agtattgctc gggtattgaa
     8401 tttatataat catttataat ttttttgatc tcagtttcag aaattttact ttcagtactg
     8461 agatcatatt tatcatcaaa ctcacttttg ttgatctttg tatcataata atttttcaca
     8521 aggtcatcac cttcctcaaa actagaacct tcaacatcaa ttgataaaat actgaatctg
     8581 cgtgacttta actttttata attatcaaca gcagtattat aaactatagt atagaaatat
     8641 gtcgagagct ttgatctttc ttcaaactta ctgccggtta tcgatttaaa taatttcata
     8701 aatgattcct gcagacaatc ttcagcttct tctttatttc taaggatctt catggtcagt
     8761 gaaaatgctt tgtcctttag ctcttcggca atgatcctga aggatgatgt atcccctttt
     8821 ttaatattat atattatttc agaatctgag agcttcattg tttttattac ttacagatat
     8881 ttttaaataa attgcataaa tcaattttca aaatcaaatc tttcttatat gtcctgttca
     8941 ccaaataaaa atatcttacc atttacagtc tcaattgaga taattgattt ctcttctccg
     9001 gatcccagac tacctttaaa tgatttattt tcctgatcca ttacagtgaa agaaaggttc
     9061 tccgttgtaa ccttgctgtt aagggattcc gcattcactt ttgcattcat ataattactt
     9121 aatccaagag acacttttcc gttaattata ttaatgttca tcccttttgt ggaatcgatc
     9181 tttccggaaa gagaaccatt tgtgatttcg cattccagtt ggcctgaata tttatcaatt
     9241 tcaacatcac cgttaatctg atggatcact aaatcatttg aaattttttc agcggtcaca
     9301 gttccgttta cattttcaat tattatagaa atacctgcgg gtacgaatat ttcataatcc
     9361 actttttgtc ttctgtcaaa gttcaaattg aaaccatcat cttcacctct ttttccaatt
     9421 actgtttcta tattaattac acttccaatg gtatcaattt ctatagtgat ttcatcaaac
     9481 ggagtatcta aatgtttttt cttaacttta atttcttttt cagcttttat cttcacaaac
     9541 cctgaatcat ccgactgctt aatatttaca tttccgttaa tattattcaa tacaagtttt
     9601 gttttacctt cagacaatac tgcatgtatt tcttttgcag attttgtata ttttttagaa
     9661 agtaatccgc agctgttgaa taaaaatccg aagattactg ctattactat aattgaagtt
     9721 ttcataattt ttaaaattca gatttataaa tcattgattt aaagtagttt catttttgtt
     9781 tttagaattg accacaagaa aataaataag gaatcccaat ccaataaatg taactattat
     9841 tcccatcatt acaccttctt ctacatcgta aatactttcg gctacgtttg caagaaaaat
     9901 accgaagccc aagaataaaa gtacaagtcc gaacttaaga gatgcatatg gattttttgg
     9961 cttggtatta tctgcgaaat aagctttcac ttcctcaggt gacagacctt tttcaatgag
    10021 tctcattttc tctctatgct ttgtgtagat ccaccagaac agtaaaaccc ctgggattcc
    10081 gaatgtaaaa actattgcaa ctaccggtat aaatgcttct aatgcattgt tcatttttta
    10141 taactccttt ccgctgattt agcgaatttg tttaggtttt aaaattttga ttttaacaat
    10201 gacaagatga aaacaatcac aaaagttacc gaaataataa tgacctcatt cattaaagta
    10261 ttgttaattt taatttctaa tagttatgac ttttatatct tcataaggtt tcacgtaata
    10321 ataatttttt tcaaaataaa accccgcagt caacagactg tccgaaaact ttggatttac
    10381 ctcccagact caaagtctca aagtttttta aattgataat aaattcctta aagccttaga
    10441 ggctttgagg taattttata aattttgttt tcgaacaagc tgtgaattgc ggggtttgaa
    10501 cttaaaaatt taaataaact ttacttcttc agacctttta ctttaaatga gatcatcatc
    10561 atcattattc cagatagtat tgcataaaat ccgaatacca tcacgagaat ctctgcacct
    10621 gcaaagacat tagcaaaaat caggatacct aatataactg tgattattcc gttaaaaata
    10681 atcagccatt cgttttgaat aacttttctt aatttgattg ctttgacgat cataaatatt
    10741 ccggctatga gtgcccagaa tgcaataaag taaagtgtaa atgagattac aaatcccggc
    10801 attattaaaa acagcagacc aaacaggata taaaatattc cttctaaaat tattgtacct
    10861 ttatcttcag aagacataaa accttcaatt attacaaaga tcccgctgaa tattgcaatg
    10921 aatccgaaat agaataacat tgttgcaagt atcattccag gagtagccaa tgccagtatt
    10981 ccgaagatca atagaagtat tcctctaata agtaataccc accatttttt ttgtaccgct
    11041 tcaagcatag ttattttttt ttgttttata aattatgtga attgagatat ttttaatttt
    11101 tttaaaaatt aattaaattt aaccggatca aaccccggaa acatcttagc tgattcatcc
    11161 aaagttgata acgtttcata tgtacaggca tcttcaaatc ttttccaaag atccagtaca
    11221 ttcgaatttt catgagcatg atttattgca tcctgtgact tccattcaaa aacttcaagt
    11281 atcgaaccat tttctgattt cattactata cattctttgt ctgtaataag tccttctttt
    11341 ctcaataccg gcatgtgatc ttttaatact tcgaggagtt ctttttcttt tccttctttt
    11401 ggtttaaaac atgctatcac tatgatcccc atattaaaat tcttaattct tagattttaa
    11461 tttataactt ttaattaaat atatgctata catttaagtt ctatagctat tggtgtagga
    11521 agtgaattaa tttctactgt agttctgcac ggctgattaa ctttaaaata ttcagcataa
    11581 attctgttat aggttgcgaa gtcatctttc atatttgtaa gaaatactgt aacatcaatt
    11641 agtttactcc agtcagagcc tgcatcttca agaatatact ttacattttg aaatactgat
    11701 cggcattggg tttcgatatc atatgaaata atattaccgc tgttgtcgag ttcgacgccg
    11761 gggattttct tagtgcctct ttcccttggt ccgactcctg aaagaaacag cagatctcca
    11821 aaccgtctgg catgtggata aagtccgacc ggctcaggag cttttgagga atttaacaga
    11881 tctgttgaat tcattttaat tgatttttgt aaattaaaat taatgaacgg taaattcaag
    11941 attgaattta taattgaaaa gtattttgtc gtatgttaaa ttataacctt ttaaagagag
    12001 ttattccgga atcagatttc taaataaaat ttttataaaa aaaatgaaaa tcttatctgt
    12061 aaatacaggc ggtccgcaga aattcatgtg ggaaggaaaa gagataacaa caagtatttt
    12121 taaatctcct gcagaaggaa aattcgaaac aactttcctt aatataaacg gtgacataca
    12181 atccgatctt aaaaatcatg gtgggttaga taaagctgtt tattcttatg accattctta
    12241 ttatgaaaca tggaaaaaag aaatggacag ggaatcctgg gagtatggtc tattcggtga
    12301 aaacttaact acttcaggtc ttacagatga ttcagtcctt attggaaatg tttatcagat
    12361 tggctctgta aaactcaggg ctgttcagcc aagatttccc tgttttaaac tgaatataag
    12421 attcggagag gatcacattc ttaacagatt ttatgatctt ggatttttcg gtacttactt
    12481 ccgagtcgta gaagaaggtt atctcaacac cggagatgaa attattctta ttcataagtc
    12541 tgatattaat ttgtcaattg ctgatatagt attttgtaaa aaatcaaaag gtgaagatca
    12601 gtataaacta aatctgatac ttgaaaatca atacattcca gacagcctga aagactccct
    12661 gagaatttat ttaaaatgat cagttgcttt tcggagatga atccgtattc ccgtcgtcgt
    12721 ataatccggt gactgaagta atatttccat taccgtcact ttcaaatctc actctgaaat
    12781 aatcaatttc actcagcata aaagtattct cagacattgg tgttaattca aatttctgac
    12841 gtccgcttct ttgataaaac aatttgccgt tttcaagata tatttttcta tcaccgtatt
    12901 cgccttcgta ttttttcaaa agagcagcat caatttccac atcattcaaa gtcgttgtca
    12961 gagactctac catccaggtg tatttcctct tcatattttc atcagtcaaa tccgctgata
    13021 ttttttctaa agcaagtata tgtgcctttg tcagagcatt aatgctgttg atctctacgt
    13081 caggttttac acctgttcct tcccagttgg tttttgtaat tggattgatc gcccttccgg
    13141 taggaatgaa aatagcaaaa ttgtcatcaa taatattcat accaccagga tgcgctccgc
    13201 cgccggtagt ttcaccaact atagttgctc ttttaagatt ttgtagattg taagaaaatt
    13261 cttccgcacc tgaaaaggtg taactgctcg ttagtacata gaccggaact tcaggctttc
    13321 tttttccttc aatatttttt aatgtccaga attcttcagt cgtgtctttt ggtctgtaat
    13381 acagatcatt tagatgaact ggttcctcac cgaataaata actcgtgatc acctgtatcc
    13441 ctgtcggatc tccgccacca ttatttctca tatcaaaaat cagtgcatca cagttctcaa
    13501 caaatgccat aactgaagct accttatctt taatttgatc tcctgatcca aattgtctga
    13561 aatcaatata tccgatattc ccgctgatcc tttctacttt cctgaatccg aaattatcaa
    13621 atttcatttc ttccagatat gtctgatcag aattttcatc ctgaccctct gactgcttca
    13681 tcatttttat ctgatcagga ctgaatctga ctctgatatg cttatctttg ctgacagatt
    13741 gcagatcttc tgtgagctga agagcaaatt tcgccgggtc gttcagatct gaatactttc
    13801 cgctttcaaa attatttaca atcaatgtcc ccatttcgct tgctttatcc tgaaagatat
    13861 aattatcatt cagcatttgg gatactttat ttactgtatt cattttcatt tcatttgata
    13921 aaactacatc attactttgt gaagtctggc ttatacctct tacctggacc aaagctgata
    13981 ttgttaataa aattattatt tttactatga tatattttct catgataaat tgttttgttt
    14041 aaaaatctta aaagttaaat attactttta tttaatgaaa ataccattgc tgcaacgaaa
    14101 gccgctttaa gcaacagcat taaaaaatta tattttcttt catttatggt atccattatt
    14161 tttgattaat ttttttaaaa ttatagctca tgcgaaaagt atctctttcg tattttgaag
    14221 gatctttcat ttccacttct tcgaatccga gatttttata taaattaatt gcaggtgtca
    14281 gcagagaact tgtatccaaa tagagattat cacactttag ctgctttacc ttttcaatta
    14341 ctgcattacc aagtttcttt ccgatctgtt ttccctgata ctcatttttc actgccattt
    14401 ttgcaagctc ataagtttta tcttctcttt taagaactgc agcagtaccg gctatttcat
    14461 ctttatactc agcaaagaaa atgtatccgc ctttattaat tatttctttt tcaggattct
    14521 caagctgttt ctcatcttcc ttttccactt taaaatattt gttgagccat tcataattaa
    14581 gttctttgaa atatttttta tgctcatttt tttttgagtt atcatattcc aggatcttta
    14641 tttcttcaag ctgtctcttt ttagactttt ccattactct tgaataaata tcattcttct
    14701 caagttcaga ctctaattta cttatgacaa ataaaacatc ataacctgtg tcactgaata
    14761 attccttaac tgaattctca atgtcctgcc atacaggaat tagctcaagc agcagatctt
    14821 caccttttgc agtaattgtc agaatctttt ttctgctgtc ctttttttcc tttactgcgc
    14881 ttatcagttt tttcttgacg agagaatttg cgatctgagt tacagccggc tgagtgtatc
    14941 caagctgatc agcaatttct gttactgtaa tagatttttt taaatttatc agataaaata
    15001 cagtgaacca ccttggttca aaatcaagtc cggtatcttt ataaattttg tcagcatcgg
    15061 aaatgaactt atccgtaagc attcttagtc ttgttccgat tgctatgtat ccaagctgtt
    15121 ttattgtatt catgagggct ttatattaaa aaattctgtt atgtatatgt ttatataagt
    15181 gcttatgcaa tttaataaat ttcattttct ttgtcaagta atgaatcagc atctattcaa
    15241 tctccttctt aacctttttt aaagactgaa ttttttataa tttgaatatc tgaaaaaatc
    15301 aaaaaagtga atttgagatc taaaggtgtt cgatcatttc aattatatca gaataaatta
    15361 ttcaaaacca cgtcaatctt atttttactg ttgacgatca ttcctttttt aattatttca
    15421 tttttcacaa atccaacctg ggatgatttt gattttactg ttaatgctct gaagtttggg
    15481 tttttgagat ctcaatataa ctggtatata ggttggtcgg gaagattttt ctcaactgct
    15541 gtcatgagca gtgtcaatcc attgatgttt cattcgatca tcggttataa acttgcatcc
    15601 ttttccgtta tcattctttt tattatttca gtcttttact ttataaaata tgttacaggg
    15661 aatactctta acttaactca atactcaatt gcagcactat ctatatgctc gctctatttt
    15721 tataaaatgg caaatcctgc attaggattt tactggctgt catcagcggt catttatcaa
    15781 cttgcaaata ttctgttcat cttactgatc atacttttaa taaaatacag cggacagaat
    15841 tcaaaattaa tcaggtcagt ttatttgatc ataatttcat ttgttataat tgcaattcag
    15901 ggatcgaatg aaatttcaat gctcctgaca atggaatttc tcggattcct ttttatttat
    15961 catctcttta aattcaaaaa aattaattat tcacttctgt tttttattgt aatttctata
    16021 attggatttt atctggtata tactgcacca gggaacggag tgagggaaag taaaaatcca
    16081 cagagtcaca tgctgttttc atcactaagc ttgtcgcttt ctgacacggc agaattaatt
    16141 ctatacagag taacagacac accgcttatt ctgttttcta tacttgttct tccatttatt
    16201 ttaaaaattt ctgaaaatca ctcagataca aaaagatatt ttgattgcaa tcctgtcatt
    16261 tcagtatttt cagtttttgt tattttgctt tcgattaatt ttgtaactta ctggactata
    16321 aataaaggta tgcttgacag aacccgaaat gtattttact ttttcttttt gatcggatgg
    16381 ttttataatt tacttgtatt ctgtaattat tttataaaat atagatctgt aaagaatttt
    16441 aaattacctg ctcttgctgt tataattatc agtttattaa ttgtaatttc ctatggcaga
    16501 aaaaacaatg tcctgcttgc ttataaagaa ttgagtaatg gcagtctgat taattttgat
    16561 catgaactat cagatcggta tgattatatc agaaaagaaa attccgatac ctgtatttta
    16621 aaacctctgt ctaatcccat acccggcctt atgttttttt atgacgttgg agcagatcca
    16681 aatgctaaac ataacaaata tcaggcatac tattatcata aaaaatttat gctcgttaac
    16741 gacaccagta aaaaagagta atttgtttaa tgtaattgtt ttcaaatctt tttactttat
    16801 ttaaattaat ttatataaat aattatgcta agatcttact taataatact ttccatattt
    16861 ctatacgtta gtgtttgtta ttcacaaaat gaaatttata tattacctgg agaaaattta
    16921 attaccgacg gacttcctga cattcctgtt tctttatcag ttgaacttga aaaatataca
    16981 gaatcaaaaa gtgctgtact ttcagactgg catcctgtca acaaggaaat gatcatgtca
    17041 actcgaatcg gtaatacatc acagcttaaa tatctttcat ctcctctgga tactggaatg
    17101 caacttactt tttttgacga acctgtccgt tcagcttcat ttgatcctgt taatggagat
    17161 ttttttcttt attacaagga caccggtgga gatgaattca gacagatcta cagatatgat
    17221 cttaaagatg gtaatacaac tatgataaca aaaggcggac gtgaacagaa tagcggtatt
    17281 gaatggctcg gcagcggaaa tcatgttgcc tatacctcta cacgcagaaa cggagctgac
    17341 cgcgatattt atattatgga tccaaagaac cctgactcag attcactttt ccttgaatta
    17401 aaaggaggcg gctggggaat actcgattgc tcagaagacg gaaagtcgct gcttctaatg
    17461 gagtatattt caaactcaat ctcttattac tggcttgctg atcttactac cggaaagctg
    17521 tccgaactta caggaaaaag tgaagaagat atttattact cgcaggcgat gtttagtaag
    17581 gatggtaatg gtctttattt tattaccgac aaagatcatg agttcaaaac acttgcattc
    17641 atggatctta aaaacaaatc agcaaaattc atttcagata atcagacagg cgatgttgaa
    17701 agttttaatc tttctgatga tggacagtcg cttgcttata ttgtaaatga atatggtgaa
    17761 tctaaacttt acgtttataa tacggtcaca ggaatttctt ctcagataaa taatttaccg
    17821 gtcggagttt acggaagttt attgtttcat aataattctg aggatctggc tctgacgata
    17881 agttcggcaa gatcttcacg tgatatctat gtatataatt taaaatcaga tgcccttacc
    17941 gcatggactg aaagcgatat gggcggaatt atccctgaag agctcagcat tcctgaactt
    18001 ataaaatgga atagctttga cggtcttgag atcagtggat tttattataa acctcatcct
    18061 aaattttcag gtaagagacc cgtgataatt gatatacacg gaggtcctgc aagtcagtcc
    18121 agaccaagct atctcggttc ttataattat tatcttaatg aactcggcat tgcaataatt
    18181 ttaccaaatg taagaggatc tgcgggattt ggaaaaacat ttctcaaact tgataacggt
    18241 tttgatagaa aaaattctgt aaaagatatt ggtgcgctgt ttgataaaat tgaattgata
    18301 cctgggcttg atgctgacag ggttatggtc atgggcggaa gttatggagg atatatgtct
    18361 ctggcaattt ccgttgacta tgctgataaa ataagaggtg cagttgatgt tgttggaatt
    18421 tcaaatttta atacgtttct taaaaacact gaaagttaca ggacagatct tcgaagagtg
    18481 gaatatggag atgagagaga tccggcgatg tatgaatttc ttgaaaagat ttcaccactg
    18541 aataatgctg aaaagatcac gaagccgctg cttattgttc agggcggaaa tgatcccaga
    18601 gtaccaagga cagaagctga gcaaatgatg aaaactataa aagatctaaa tggaactgtc
    18661 tggtatctcg aagcaaaaga tgaag
//
LOCUS       DAORVQ010000044        18599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1223_length_18599_cov_6.008682, whole genome shotgun sequence.
ACCESSION   DAORVQ010000044 DAORVQ010000000
VERSION     DAORVQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 18599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18599
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_18599_cov_6.008682"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..917)
                     /locus_tag="PLX80_05440"
     CDS             complement(330..917)
                     /locus_tag="PLX80_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQY20272.1"
                     /translation="MSKQNKPKTPEKKTGSTGSSKYILIAVLILIAGYFFYDNVLREE
                     PVKPPVFVDPKEKVKSTTEPQFKREGELEFIRSAGKKDSAVKKIDIELALDDAEREQG
                     LMYRKSMDDNKGMLFIFDTDELQSFWMKNTIMPLDIMFVNSDFKIVKIHKNTTPFSEN
                     SLPSDKPSKYVVEVAAGFSDRFGIKEGDKILFKKD"
     gene            complement(957..2027)
                     /locus_tag="PLX80_05445"
     CDS             complement(957..2027)
                     /locus_tag="PLX80_05445"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQY20273.1"
                     /translation="MKDLKYKTLQYLDRNESQYGPSPKCYEYLKTVGIEELAWYSRDF
                     SRGVKSKLSERIAKDFGFRENQIIVSYGSEDILKQLIHRYLSRGEKVLIPREAWWYYK
                     KVAYEVGGFNVEYPMKKGKIDGVDSFLYDLDQMIKIYERERPRVVIIASPNNPTGNMI
                     SKKDLGKFLDVCRGAIVMIDEAYWGFGSYDNSYVKPYLDEFPNLIICRTFSKYFALAG
                     TRMGFSFAGNNLEELKGYTTRYLGYNLVSEGLCLTAYDDMKYYEKVTKLMQEDKEYFY
                     KEFKKLKGFTPYKSFANFMLVDMPAGIRKDLDKFLKDRNLLIKFLDEEAFKTESRITL
                     GTREQNYYLMESIKEFIKKTKY"
     gene            complement(2064..2843)
                     /locus_tag="PLX80_05450"
     CDS             complement(2064..2843)
                     /locus_tag="PLX80_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY20274.1"
                     /translation="MKLNTIIIEETGENNQTKKIILNRPEKRNSLDEEMIAELTQTFT
                     EISSDKETKSVILTGAAGNFCSGLYLDYLQKISEYDILENKADSQKFRDLLFTIYNCK
                     KPVIAMVEGYALAGGCGIASACDIIVAAEDSQFGYTEVKIGFIPAIVMIFLLKRVSES
                     HARDLLLTSKFISGEEAKRIGFANYTSTAAELENTTMEICNGLNKLPLSSISLTKEMF
                     RNVSSMSLESSLDYAVDMNAITRMTDECKNGVKNFLMKIKK"
     gene            complement(2850..3671)
                     /locus_tag="PLX80_05455"
     CDS             complement(2850..3671)
                     /locus_tag="PLX80_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY20275.1"
                     /translation="MNEILKVLDLNFLYSEETKNSEYTFGLKDINIKVIEKDLISVIG
                     KNGSGKSTLIKLFSRINNPDSGKVFFRDAEILKYDKKDLSKFISYLPQMNDLTHFDSD
                     VIDFLMLGRYPYKNFLDFGYTKNDTDEVLICSKITGIENLLAKKINTLSGGERQKVLL
                     TLSLVQLGTADDLTGKLLIADEPLTHLDINHQIEIMNILKTLNEKGLTIIIVLHDLNL
                     ALKYTNKAMLISNGRVVKFSETDKVITEEMLKEHFFIDSKILNYEKNFFINYLPN"
     gene            complement(3675..4160)
                     /locus_tag="PLX80_05460"
     CDS             complement(3675..4160)
                     /locus_tag="PLX80_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY20276.1"
                     /translation="MKLFLARHADAIQYTTDTVTNDELRFLTSGGRIKENETADFLKE
                     YLTDISIILTSPLIRTVQTAEIIASKINYTGEISLVNELKFETPLSPLIDLIKTMSNY
                     SSLLMVGHEPKLGNLVKALTSISLPSFSKSGVCYIDYNTDKDIFHFGWYFDPKKMMII
                     D"
     gene            complement(4173..5459)
                     /gene="mtaB"
                     /locus_tag="PLX80_05465"
     CDS             complement(4173..5459)
                     /gene="mtaB"
                     /locus_tag="PLX80_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQY20277.1"
                     /translation="MKSVSLHTLGCKLNYSETSTIGKGFSQKGFSLKEFGEPADVFVL
                     NTCSVTENADRECRQIVRSVLNKNPQTYVIVTGCYAQLQPNEIASIEGVDLVLGISEK
                     MKLMDYIDNIEKKELSCIHRDSVEKANDFFEAFSSDTDSRTRAFLKIQDGCNYKCSFC
                     TIPLARGASRSLAPDKVLDNAKKIIDAGYREIVLTGVNTGDYDFENYRLIDILNILEG
                     TEIDRIRLSSIEPNLITDEIISFAKNSEKFCNHMHIPMQSGDNEILRSMRRRYNREYY
                     RDTVLKLKDEVKDICIGADVIVGYPGETEEHFLNTEKFIRELPVNYLHVFSYSERRDT
                     DAVLLPGRVEIADRKKRSSILRRLSDSKKYEHYNKFIGTEQTVLFESDKDDYAEGLSE
                     NYIRVKVNKSNLENKIRKVKLTETDGIKPVTAELLS"
     gene            complement(5574..5996)
                     /locus_tag="PLX80_05470"
     CDS             complement(5574..5996)
                     /locus_tag="PLX80_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQY20278.1"
                     /translation="MKIIQKEIRLKQKARGFHIITNEILDAVPEIKEMSSGMMNVFIK
                     HTSASLSINENADPTVRIDFETHFNKMVPEDGEDYLHTAEGPDDMTSHIKSSLLGCSL
                     NIPVTNGELNIGIWQGIYLCEHRNHGGQRDIVITLTGI"
     gene            6051..6731
                     /locus_tag="PLX80_05475"
     CDS             6051..6731
                     /locus_tag="PLX80_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY20279.1"
                     /translation="MKMLIKEIVLCTSIDLDELKNFYSGLLGLKVLKNEDQTISFTAG
                     SSILTFENKSDYPDPKYHFAFNIPSNKFKEAKEYLTASGIGLVSLDGNDEFDFKSWNA
                     HSVYFYDPAGNILEFISRHNLKNDSDHKFSGDSILCISEIGLPVKNVNEFFGEVQNTF
                     EIPQFSGDKSTFAALGDDNGLFIIVNTGRIWYPDCGPAKEYPLKVLINSDQFKEIKYS
                     EYQIFSET"
     gene            6884..8167
                     /locus_tag="PLX80_05480"
     CDS             6884..8167
                     /locus_tag="PLX80_05480"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQY20280.1"
                     /translation="MPEIYKEPAPYRRGEVNPFESMMVRFDKACELYNLDKGLYNYLA
                     TPVKQVIVSIPVTMDDGTLQVFEGYRVIHDNILGPSKGGIRYSPDVDLDEVKALASWM
                     TWKCAIANIPFGGAKGAVKCDPKNLSQRELELITRRYTVNMLEVFGPDSDIPAPDMNT
                     DEQIMAWIMDTYSMHKKETVTGVVTGKPIIIGGSKGRREATGRGVMIATMAALKTLEI
                     DPTSTTCVIQGFGNVGSVSAQLLNEKGIKIVAISDVSGGYHDENGIDVNKAIEYVTLN
                     KTLENGNFGRSVTNEELLELQCDILVPAAKEDQISAHNANNIKAKLIVEGANGPISAD
                     ADEIIAGKGIRVIPDILANSGGVIVSYLEWVQDRIGYFWDEDDVNNRLNRMMTEAFDV
                     VYESSIKYNTTLRLGAYIHAIDKVSRVLKLRGIYG"
     gene            8171..8791
                     /locus_tag="PLX80_05485"
     CDS             8171..8791
                     /locus_tag="PLX80_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20281.1"
                     /translation="MKFKNLLIILFFFISGGVCFCQDTPGNLYLIDKLMDESFQSLEN
                     KLVILGNDKFYEIEIDQNKPDEIYFLYKLRDKFRNYKFIINENFDSTNYIIKISNISI
                     KPVYSESQSTDITGNKLTKREIRYSYNYEILDQNSIRVLYNDKIVKKSTGNFDSNNKD
                     QIEDSRYTFTQGDLPAESDVNSYVFPAIIVAVSAAAIILFFTIRSN"
     gene            8853..9611
                     /gene="bamD"
                     /locus_tag="PLX80_05490"
     CDS             8853..9611
                     /gene="bamD"
                     /locus_tag="PLX80_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQY20282.1"
                     /translation="MNKRIFSVLIIFVSSILYSCSSSTNEGNINSDVPESAYFAAKSN
                     YDQEDYIDAIDDFNLIKLKFSGTSVIDKSIYYLGMSYYKREEYILAIYEFEVLIKNYP
                     TSKFSETGRYRLAMSYYKLSPSYSLDQTYTRYAITEFQTFLDLYPNSKHASSVNKRIA
                     EMKDKLAYKILKSAELYFTMDSYKSSLVYYSEILDDYFDSKYADDALYGKIQVQIIKK
                     NYDEAKKDIKRFEDIFFASEYINKVRSLKSKIPA"
     gene            9620..10096
                     /locus_tag="PLX80_05495"
     CDS             9620..10096
                     /locus_tag="PLX80_05495"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQY20283.1"
                     /translation="MKTVKSSQVEMIELVLPNDTNVLGNLLGGRLMHWIDIAAALAAA
                     RHCNSVAVTAAVDNLVFHHPIKLGNIIRLKASVNRAFNTSMEVGVRVEIEEIKSGEIS
                     HSNSAYLTFVNIDAVTNKPVIVGEIIPESEIEKRRYDQALRRRNQRLSEKHGILNE"
     gene            10120..10755
                     /locus_tag="PLX80_05500"
     CDS             10120..10755
                     /locus_tag="PLX80_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY20284.1"
                     /translation="MKSIELSAENISKSYSGKIIFKNFSINLKSGDSVSILGRNGSGK
                     STLIKLLTGLIASNKGKVILKVNGSTVPQNLWYSQIGLISPYLNLYDELTGIENLEFF
                     CSLRNNSRDDMKEKIDFYLNKVSLFDKRNEPLKNYSSGMKQKLKLIFAIINEPGILYM
                     DEPRSNLDTEGIDIICQTAEEQKKNGILIVATNDTDDIQLCEKSINIEDYK"
     gene            10760..11425
                     /locus_tag="PLX80_05505"
     CDS             10760..11425
                     /locus_tag="PLX80_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQY20285.1"
                     /translation="MLLKNSFLVFKKDLSSELRTRYVINSLLMFVLVTISIIKFALGD
                     EKVDNEILTGLLWIAIFFTSVSGLSRTFVKEEEKETSTALKLSTGTDSVIIGKLFFNL
                     ILTFALNLLILILFILITEYQIKNLSGFLVVIVLGNLGLVSASTIIAAIISKANSKGT
                     LYPVLSFPILLPLLITVINATKLASLGVETDKLFGEIQILVSYFVVVTVTSLMLFKFI
                     WED"
     gene            11519..12181
                     /locus_tag="PLX80_05510"
     CDS             11519..12181
                     /locus_tag="PLX80_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HQY20286.1"
                     /translation="MNKFITYLIFIIISAVIILGLALPIPDIPALGDKARVLYFHVPM
                     SWISVLAFFMSMWYSIRYLKTRDINYDIKSYSSAQLGFLFCILATATGSLWAKFSWGS
                     FWNWDPRQTSIFILLLIYGAYFALRSAIESEDQRAKLSSVYAIIAAVTVPFFVFIMPR
                     IVESLHPDPIINTQGKINMDSSMLTIFLSSLVGFTMFYLWMLNLKIRIEKLIYKKLSL
                     IE"
     gene            12186..12323
                     /locus_tag="PLX80_05515"
     CDS             12186..12323
                     /locus_tag="PLX80_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HQY20287.1"
                     /translation="MTSLYDFLETNSLYIVMLIVLIIWIGIFGYMRSLDKKVKKLENN
                     E"
     gene            12421..12591
                     /locus_tag="PLX80_05520"
     CDS             12421..12591
                     /locus_tag="PLX80_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20288.1"
                     /translation="MKTFSPPTCDLKHVGIVVVDQKDYENDTINSSAALKLIGSTSTE
                     KINSQTKNSIRL"
     gene            complement(12622..14148)
                     /locus_tag="PLX80_05525"
     CDS             complement(12622..14148)
                     /locus_tag="PLX80_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQY20289.1"
                     /translation="MNQKEIKTFGQLKKSGYKVLTVKDEIRKNLIKKIETKGTFFKGI
                     IGYENSVIPSLMNSLLSKHDIILLGLRGQAKTKIARMIPELLDEYIPIVKGSEINDNP
                     FQPISKYSVDMINSQGDETEIEWISREQRYSEKLATPDVNIADLIGDIDPIKAANQRL
                     HYSHEGAIHFGIIPRTNRGIFVINELPDLQPRIQVGLLNILQEKDIQIRGFNIRIPLD
                     ILMIFTANPEDYTNRGNIITPLKDRIDSQIITHYPKVLEDGIKITESNSWTDRNDKKN
                     IHIPYYFREIIEMTAMQARASEFVDQKSGVSARLTISAMENLISNAERRSIINSEDII
                     MPRMCDVLSILPGITGKVELVFEGEQEGSLKVAKALISRSVREIFKKYFPDPSLRLKK
                     NAQEKPQRDPYIEITEWFQFGGSVNIKDDMSFKNYFAELKKVDGLEIFVRKYPQFFEN
                     DQELASLMEFVLDGLHQNSKIAKDEIDSVIKYKDMVGSMFSGSKDASEFGGFEDDYYN
                     "
     gene            complement(14211..14648)
                     /locus_tag="PLX80_05530"
     CDS             complement(14211..14648)
                     /locus_tag="PLX80_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQY20290.1"
                     /translation="MKTLKDLIKDRPLHFVKSGMKIIDVAKFMGLHNIGAVPVLEQSE
                     KLKLIGIFSERDLLRKCIAKEIDLFNTSVDDVMTKKVIVIESHDTPEYCMQIMKQEKI
                     RHMPVIDGQDLKGMISIRDLMTYDMSQKEEKIEMLNSYIQFSG"
     gene            complement(14885..18424)
                     /locus_tag="PLX80_05535"
     CDS             complement(14885..18424)
                     /locus_tag="PLX80_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HQY20291.1"
                     /translation="MQNSLSTISIYNFFEKKLSAVNKKELLFSFLRNTITSLIIFLLL
                     GFLITVLESVFRFGIFTRTFLYWGFLSVSITTITYFLLNFLLKSSGIIGNFNLISYSR
                     KVGDHFEDIKDKLSNSLSLVMPGNSDRKNSAVSDELIFEDIKSINNIAGNKDLSSFIK
                     FSSLKKLILILLSIIVFITASFLIFPKNLNGAVKRLVNHNYFFLDNDLGITFEIIPGD
                     IEIAAGEDVIIGVTINSTSNDLNIKEINLYTKQVSADGYEMLSDPVKLISESGNNFTG
                     TIKNTINELIYFAEYKGIRSEEYTINVSEYPVIKNFTVTVTPPEFTGIPSKTLPDNEG
                     NIFCPEGSTIEFNINASTELSYAGIDLNGNPVSFEFNGTSAKGSIKADQSSSYRFILK
                     DLNGGVNKNFKQYTIKVMPDEPPKITIIEPSESNYELKGERELIVRSRISDDYGFSKL
                     VLGYRKLKLLSGSGSAPEFNYINIEIKNPNATSLEVPYVWNVSGINLRSGESAEYFTE
                     VTDNTGKSTRSEIKTIQYRSQAEVLKETETMTKELKTEMQSAYDQMEELSKNIEDIKK
                     EFQKNEELGLNEDSKKQMEQKLDNFQKNMNSAQNKLDQNMEEMKKNNMLGEKTLEQFM
                     ELQKMFNKINSPELQKMLEKLKEALKKNNQEELKDAMKNFKFDEEAFKKYMEKAMELM
                     KKIENMQKFGELTQKLEDIAKQQEQLKKETENSQNSDKNKMDELSQKQKDIKEQTKDF
                     NSELQKLIDEMNKMKDQLSPEELEKIQKEMNKKDTENKMQKSSDELKSSQKSNSEKTQ
                     EEIMDELNNMDKKMKAALNEMMDSQDMNNKLKEKLEDIKKKLEEMSRRQQELKDKTDE
                     LDKDEKEEFEKNQKDQNDLQKQLSESIDDLMNASKMGMKMSPELGKELGDAYNKMEKA
                     GKELGEMKKQDASSDQKKAKESLDNAAKMLGNMLGKMGKDGKGGEDGKDGKDGKGNKP
                     GSGNMGQMMDRLGEIIAQQMGMNGKSGKMGMNGESGENGKSGKDGKGNKPDNMSEEQR
                     QEMQRLSMEQQQIQKSLEMLNEELKREQENSGEKVLGDLDQIQKEMQEIVKDLSEYNV
                     DDKLIEKQNRILSRLLDARLSQREKDFEPKRESRPGENVSRNSPPEIILSGPNSYNAL
                     KEDFLRLDKEGFNEDYENLISKYLMELKKNDLK"
BASE COUNT         5754 a         3273 c         2967 g         6605 t
ORIGIN      
        1 gaaaaaccga cataaaatta attatttaac ctctaagact ctaagactct aagtctctaa
       61 ggctctaaga ctctaaggct ctaaggctct aaggctctaa gtttctgaga ctctgagact
      121 ctgagactct gaggcttgaa ggttcaatga aaattaaatt taattatttt tattgttaga
      181 gccttaagat aattggaatg tattcaaaaa cactgagaat ttggaaaagg gcttctttca
      241 gtttgtaatg tatttagtaa ttgtgtaatt tcagttatta actattaact attaactatt
      301 aactattaac tattaactat taactattcc tagtcttttt taaaaagtat cttatcgcct
      361 tccttaattc cgaacctatc tgaaaatcca gcagccacct cgactacata ctttgatggt
      421 ttatcagaag gcagagaatt ttctgaaaac ggcgtagtgt ttttgtggat ctttacaatt
      481 ttaaaatcag aatttacaaa cataatatcc agcggcatga tagtattctt catccagaat
      541 gactgcagtt catcagtatc aaaaataaat aacatccctt tattatcatc catggatttt
      601 ctgtacataa gtccctgctc tctttctgca tcatccaaag ccagctcgat atcaattttc
      661 tttacagcac tgtccttttt tccagctgat ctgataaact caagttcacc ttctctttta
      721 aactgaggct ctgttgtact tttaactttc tctttcggat ctacaaagac aggcggtttg
      781 accggctctt cacgtaatac attatcataa aaaaaataac ctgcgatcaa aattaacacg
      841 gcaattaaaa tatatttgga agatccggta gaaccggttt tcttttcagg agtcttaggt
      901 ttattctgtt ttgacaaatt aaaaagatct gatttaaatt attaaaatta atattattaa
      961 tatttagttt tcttaataaa ttcttttatt gattccataa ggtaataatt ttgctcgcgt
     1021 gttccgagtg taattctcga ttcagttttg aatgcttcct catcaagaaa ctttatcagc
     1081 agatttctat ctttcaaaaa tttatcaaga tcttttctga taccggcagg catatctacc
     1141 agcataaagt ttgcaaatga tttgtaaggt gtaaatcctt ttaacttttt gaattcttta
     1201 taaaaatatt ctttatcctc ctgcataagc tttgttactt tttcataata tttcatatcg
     1261 tcataagctg tcagacaaag accctctgac accagattat atccgaggta tcttgtagta
     1321 taacctttca gctcttcaag attattaccg gcaaagctga atcccatacg tgtcccggca
     1381 agtgcaaaat atttcgaaaa cgttctgcag ataatcagat tcggaaattc atcaagataa
     1441 ggttttacgt aactgttatc ataagaaccg aatccccagt acgcttcatc tatcataacg
     1501 atcgcgcctc tgcaaacgtc gaggaatttt ccgagatcct ttttagagat catatttcct
     1561 gttggattat tcggtgacgc gattatcaca actcttggtc tttctctttc atagatcttt
     1621 atcatttgat caaggtcata aagaaatgaa tccacgccgt ctatctttcc ttttttcatc
     1681 ggatattcta cgttaaatcc gccaacttca taagctactt ttttgtaata ccaccatgcc
     1741 tctctgggaa ttaaaacttt ttctccgcgt gaaagatacc tgtgaataag ttgcttcaga
     1801 atatcttcac ttccgtaaga aacgattatc tgattttccc tgaatccaaa atctttcgcg
     1861 atcctctcgg ataatttcga ttttactcct ctcgagaaat ctcttgaata ccaggcaagt
     1921 tcttcaatgc caactgtctt tagatattca taacactttg gtgacggtcc gtactgcgat
     1981 tcatttctgt cgaggtattg cagtgtttta tattttaagt ctttcatttc agatataatt
     2041 atttattaaa attagtatta aaattatttt ttaattttca tcagaaaatt ctttacaccg
     2101 tttttgcatt cgtctgtcat tcttgtaata gcattcatat cgactgcata atcaagtgaa
     2161 gattctaaac tcattgaaga tacatttctg aacatttctt tagtgagact gatcgaacta
     2221 agcggaagtt tattcagacc attacaaatt tccatcgttg tattttccag ttcagcagca
     2281 gtcgaagtat aatttgcaaa tccaattctc ttagcttctt ctcccgaaat aaattttgaa
     2341 gtaagcagca gatctcttgc gtgggattct gaaactcttt tcagaagaaa tatcataact
     2401 attgcaggta tgaatccgat ctttacttca gtatatccaa actgtgaatc ttcagcagcg
     2461 acaataatat cacaggcact tgcaataccg cagccgcctg caagagcgta tccttccacc
     2521 attgcgatca caggtttttt acaattgtaa attgtgaaca gcagatcacg gaatttctgt
     2581 gagtcagctt tattttcaag aatgtcatat tcggatatct tctgcagata atcaagataa
     2641 agacctgaac agaaatttcc tgcagcacct gtaagtataa cactttttgt ttctttgtca
     2701 gaagagatct cagtgaaagt ttgcgtaagc tcggctatca tttcttcatc aagcgaattt
     2761 ctcttttcgg gacggtttag tataattttt ttagtctgat tattttcacc cgtctcttca
     2821 ataatgatag tatttaattt catgtaactt tagtttggca gatagtttat aaaaaaattc
     2881 ttttcgtaat ttaatatctt tgaatctata aaaaaatgtt ctttcagcat ctcttctgta
     2941 attactttat cagtctcaga aaatttcact actctaccgt tactgatcag cattgcttta
     3001 tttgtatatt ttaaagccag gttgagatca tgcagaacaa taataatagt gagtcccttt
     3061 tcatttagag ttttcagaat attcataatt tcgatctgat gatttatgtc aagatgtgta
     3121 agcggttcat cagcaattaa aagcttgcct gtcaggtcat ctgcagtacc aagctgaacg
     3181 agtgaaagtg tcagaaggac cttttgtctc tcaccaccgg aaagagtatt tattttctta
     3241 gcaagtaaat tttcaatacc ggtgatcttc gaacagatca aaacctcatc agtgtcattt
     3301 ttagtataac caaaatccaa aaaattttta tacggatacc tgccgagcat taaaaaatct
     3361 ataacatcag aatcaaaatg tgtaagatca ttcatctgcg gaagatagga aataaatttt
     3421 gaaagatcct ttttgtcata tttcaaaatc tctgcatctc tgaaaaatac tttacctgaa
     3481 tcaggattat taattcttga aaaaagtttt atcaaagtgg attttcctga accatttttt
     3541 ccgatcacgg agatcagatc tttttctatt acttttatat taatgtcttt taatccgaaa
     3601 gtatattcgg aattttttgt ttcctcagaa tatagaaaat tgagatccag tacttttaaa
     3661 atttcattca tatttcaatc aattatcatc attttttttg gatcaaaata ccatccgaaa
     3721 tgaaaaatat ctttatctgt attataatca atataacaaa cacctgactt cgaaaatgaa
     3781 gggagactga tgcttgtgag agcttttacc aaatttccaa gcttgggctc atgaccgacc
     3841 attaataatg aagaatagtt cgacatggtt tttatcagat cgataagcgg cgataaagga
     3901 gtttcaaatt tcagttcatt tacaagagaa atttcaccgg tgtaattaat ttttgaagca
     3961 attatttctg cagtctgaac agtgcgtatc agaggactgg taagtataat actaatatca
     4021 gtcagatact cttttaaaaa atcagctgtt tcattttcct tgattctccc tccggaagtt
     4081 aaaaatctga gctcatcatt tgtgacagtg tcagttgtat attgaattgc atcggcatgt
     4141 cttgcgagaa ataatttcaa ttgtttgaat ttttatgaaa gcagttcagc agtgacaggt
     4201 ttgatcccgt cagtttcagt aagttttact tttcttattt tattttcgag attactttta
     4261 tttactttga ccctgatata attttctgac agaccttcgg cataatcatc tttatctgat
     4321 tcaaacaaaa ctgtctgttc tgttccgatg aatttattgt agtgttcata ttttttactg
     4381 tcggaaagtc ttctcagaat actgcttctt ttttttctgt cagcaatctc aactctgccg
     4441 ggtaacagaa ctgcgtcagt atctctcctt tcggaataac taaaaacgtg aagataattt
     4501 accggaagct cacggataaa tttttcagta tttaaaaaat gttcttctgt ctcacccgga
     4561 tatccgacta ttacatctgc gccgatgcat atatctttta cttcgtcttt taattttaaa
     4621 acagtatctc tgtaatactc acggttatat cttcttctca tggatcttaa tatttcattg
     4681 tcaccgcttt gcatggggat gtgcatatga ttgcaaaact tttctgaatt ttttgcaaat
     4741 gaaattattt catcagtgat aagattcggc tcaatcgagc tgagccttat gcgatcaatt
     4801 tcagtccctt caagtatatt caaaatgtca atgagccgat aattttcaaa atcataatcg
     4861 cctgtattta cacctgtgag gacgatctct ctgtatccgg catcaataat ttttttcgca
     4921 ttatctaaaa ctttatccgg agcaagactt ctcgatgcac ctcttgcaag aggtatggta
     4981 caaaatgaac atttataatt acacccatcc tgaattttca gaaaagctct ggtcctcgaa
     5041 tctgtatcag atgaaaatgc ttcgaaaaaa tcatttgctt tttcaacact gtctctgtgg
     5101 atgcatgaca gttccttctt ttcgatatta tcaatataat ccatcagttt cattttctca
     5161 ctgatgccaa gaacgaggtc gacaccttcg atggatgcaa tctcattcgg ctgaagctgt
     5221 gcatagcaac ctgtcacgat cacataggtc tgaggatttt tattcaggac acttctgacg
     5281 atctgtctgc attccctgtc tgcattttca gttacagaac aggtattcaa aacaaagacg
     5341 tcagcgggct cgccgaattc tttcagactg aatccttttt gtgagaatcc ttttccgatg
     5401 gttgatgtct cgctgtaatt taatttacag ccaagtgtat gtagagaaac gcttttcatt
     5461 tggtaaattt acaaaaattg aagtgtaatt agaatataga aaaattactt tatgggaaaa
     5521 tttttttacg cgttcagatt ttcttttaac ttaataatga aattcagata aaattaaatc
     5581 ccggtaagcg taatcacaat atccctttga ccaccgtgat ttctatgctc acaaagataa
     5641 attccctgcc aaataccaat attgagttca ccatttgtga caggaatatt caaactgcat
     5701 ccgagtaaag aagattttat atgtgaagtc atgtcgtccg gaccttcggc agtatgaaga
     5761 taatcttcac cgtcttcagg gaccatttta ttaaagtgag tttcaaaatc aatccgcacg
     5821 gtcggatctg cattttcatt tattgaaagg gaggctgaag tatgtttaat aaaaacattc
     5881 atcatacctg agctcatttc tttaatctca ggaacggcat ccagtatttc atttgtaatg
     5941 atgtgaaatc cccgtgcttt ttgtttaagt ctgatctctt tctgaataat tttcaattaa
     6001 atgggagttt agtttgtgat ataatttcta tatttatcaa aataaatttt ttgaagatgc
     6061 taataaagga aatagtttta tgtacatcaa tagatttaga tgaattgaaa aatttttact
     6121 caggcttact cggactaaaa gtgttgaaaa atgaggatca gacaatctca ttcactgccg
     6181 gcagttcaat acttacattt gaaaataaat ctgattatcc ggatccgaaa tatcatttcg
     6241 cctttaacat cccatcaaat aaatttaaag aagcaaaaga atatttaact gcttcaggaa
     6301 tcggactagt gtcacttgat ggaaatgatg agtttgattt caaaagctgg aatgcacatt
     6361 cagtttactt ctatgatccg gcaggcaata tactggaatt tatttcgaga cataatttaa
     6421 aaaatgattc tgatcataaa ttctcaggtg attcaattct ttgtataagt gagattggac
     6481 tgcctgtaaa aaacgtaaat gaattttttg gagaagttca gaacacattt gaaattcccc
     6541 aattcagcgg ggataaaagt acttttgcag ctttgggaga tgacaacgga ttatttataa
     6601 tagtaaacac cggaaggata tggtacccgg attgcggtcc ggctaaagaa tacccgttaa
     6661 aagttttaat aaattcagat cagtttaaag aaattaaata ttctgaatac cagatattct
     6721 ctgaaactta aacatttgat tcaaagtatc agaaaaattc acactctgaa gcttcaattt
     6781 attgaaaaaa attaccgaat agctaaatat actaacttga tataaaaatt gcattatgtt
     6841 aattttatgc ttaatatttt ttataattct aatttcaaac taaatgccag aaatatataa
     6901 agagcctgct ccatacagaa gaggggaagt aaatcctttt gagtcaatga tggtaagatt
     6961 tgacaaagcc tgcgaacttt acaatttgga caaaggtctt tacaattact tagccacacc
     7021 ggttaagcag gttattgttt ctatacctgt tactatggat gatggaacac ttcaagtctt
     7081 cgaaggttac agagtaattc acgataatat tctgggacca tccaaaggtg gaataagata
     7141 ttcacctgat gtggatctgg atgaagtaaa agctcttgct tcatggatga catggaaatg
     7201 tgcgatagca aatattccat tcggtggtgc aaaaggtgct gtgaaatgtg atccgaaaaa
     7261 tttatcacag agagaactgg aactgatcac aagaagatat acagtaaata tgctggaagt
     7321 atttggtccc gactctgaca ttccggctcc ggatatgaat actgatgaac aaataatggc
     7381 gtggataatg gatacataca gtatgcataa aaaagaaact gtcactggag ttgttaccgg
     7441 taaaccaatt attatcggcg gatcaaaagg aagaagagaa gcaacaggca ggggagttat
     7501 gattgcaaca atggcagcct tgaaaacact ggagatcgat cctacttcta ctacctgcgt
     7561 aattcaggga tttggaaatg taggctctgt aagcgcacag cttttgaatg aaaaaggaat
     7621 aaaaatagtg gcaatcagtg atgtctccgg aggttatcat gatgaaaacg gaattgatgt
     7681 aaacaaagca atagaatatg taacgttaaa taaaactctt gagaatggaa atttcggaag
     7741 atctgttaca aatgaagaac ttctcgaact tcagtgtgat attcttgttc ctgctgcaaa
     7801 agaagatcag atctctgcac ataatgcaaa taacatcaaa gctaaattaa ttgttgaagg
     7861 cgcgaatgga ccaatttctg ctgatgctga tgagatcatt gcaggaaaag ggatcagagt
     7921 gatcccggat attcttgcaa actcaggcgg agtaatagtt tcttatcttg aatgggtaca
     7981 ggacaggatc ggatatttct gggatgaaga tgatgttaat aacagactta acagaatgat
     8041 gacggaagct ttcgatgttg tatatgagtc atctattaaa tataatacaa ctttacggtt
     8101 gggagcttat atacatgcaa ttgataaagt ttccagagtt ttaaaattaa gaggaattta
     8161 cggttagaaa atgaaattta aaaacctcct gattatattg ttctttttta tttcaggagg
     8221 agtttgtttc tgtcaggata ctccgggcaa tctttatctc atcgataaat tgatggatga
     8281 gagttttcag tcattagaaa ataagcttgt tattttaggc aatgataagt tttatgaaat
     8341 tgaaattgat cagaataaac ctgatgaaat ttatttttta tataaactca gagataagtt
     8401 cagaaattat aaatttataa taaacgagaa ttttgattcc acaaattaca ttataaagat
     8461 cagtaatatt tctattaaac ctgtttattc tgaatcacaa tctacagata tcaccggtaa
     8521 taaattaaca aaaagagaga tcagatattc ttataattat gaaattcttg atcaaaatag
     8581 tatccgggta ttatataatg ataagatagt aaaaaaatca acaggtaatt ttgattcaaa
     8641 taataaagat cagatagaag atagtcgata tacttttact cagggggatc tgccggctga
     8701 gagtgatgta aacagttatg tttttccggc gatcatagtc gcagtttctg cagctgctat
     8761 aattttattt tttacgatca gaagtaatta aaatatttaa aactgcaatt ttgttctgaa
     8821 aattattttg cagtaattat tataaaaatt tattgaataa aagaattttt tcagtactga
     8881 taatatttgt ttcatcaata ttatattcgt gtagttcatc tacaaatgaa ggaaatatta
     8941 acagtgacgt tcccgaatca gcatattttg cagctaagtc aaattatgat caggaagatt
     9001 atattgatgc gattgatgat ttcaatttaa ttaaacttaa attttcgggc acaagtgtta
     9061 ttgataagag tatatattat cttggtatga gttattataa aagagaagaa tacatacttg
     9121 cgatttatga gtttgaagta cttataaaaa attatccaac cagtaaattc tctgaaacag
     9181 gaagatacag actagccatg agttattaca aactttcacc ttcgtatagt ctagatcaga
     9241 catatacaag atatgcaatt actgaatttc aaacttttct tgatctgtat cccaacagta
     9301 aacatgcgtc atcagtaaat aaaaggattg ccgaaatgaa agataagctt gcttataaaa
     9361 ttttgaaaag cgcagagttg tattttacta tggacagtta taaatcttca ctggtatatt
     9421 acagtgagat acttgacgat tattttgatt ccaaatatgc tgatgatgct ctttacggaa
     9481 agatacaggt tcagatcatt aaaaaaaatt atgatgaagc taagaaagat ataaaaagat
     9541 ttgaggatat tttctttgca agtgaatata taaataaagt cagatcatta aaaagtaaaa
     9601 tcccggcatg acaattatct tgaagaccgt taaaagttct caggttgaaa tgatagaact
     9661 tgtccttcca aatgacacta atgttctggg gaatttatta ggcggaagac taatgcactg
     9721 gattgatatt gctgctgcat tagctgccgc cagacattgt aattctgttg ctgttactgc
     9781 tgctgtagat aatctggtat ttcatcaccc tattaaactt ggaaatatta ttcgtctaaa
     9841 ggcttctgtt aacagagctt ttaatacatc aatggaagtc ggtgtcagag tagaaattga
     9901 agaaataaaa tcaggcgaaa tttcacattc aaacagtgca tatctgacat ttgttaatat
     9961 tgatgcagtt acaaataaac cggtgattgt aggcgagata attcctgaat ctgaaataga
    10021 aaaaagaaga tatgatcaag cactcagaag aagaaatcag agactgtctg agaagcatgg
    10081 tattctaaat gaataattta tcacttccat atcctgaata tgaaaagtat tgagctctct
    10141 gctgaaaaca tttcaaaatc ttattcagga aaaataatat tcaaaaattt ttcgatcaat
    10201 ctgaaatcag gtgattcagt ttctatactc ggcaggaatg gttcgggtaa atccactctc
    10261 ataaaacttc tcacaggtct catagcttct aacaaaggta aagtaattct taaagtaaat
    10321 ggatctactg taccccaaaa tttatggtat tcacaaattg gtctgatctc tccgtatctg
    10381 aatctctatg atgagcttac aggtattgag aatcttgaat tcttttgcag cttaagaaat
    10441 aattcacgtg atgatatgaa agaaaaaatt gatttttatc ttaataaagt cagtctcttt
    10501 gataaaagaa acgaaccatt gaaaaattat tcctcaggta tgaagcaaaa gctcaaactt
    10561 atttttgcaa taattaatga acccggaatt ttatatatgg atgagccaag atcaaatctg
    10621 gatactgaag gaattgatat catctgtcag actgcagaag agcaaaagaa aaatggaatt
    10681 ttaattgttg caacaaatga tactgacgat attcagcttt gtgaaaaaag cattaacata
    10741 gaagactata agtagaatta tgcttttgaa aaattcattt ctcgtattca aaaaagatct
    10801 cagttcagag cttaggacga gatatgtaat aaattctctg ctgatgtttg tacttgttac
    10861 catctctatc attaagtttg cattaggaga tgagaaagta gataatgaga tccttacagg
    10921 tttactctgg attgcgattt tttttacatc tgtaagtgga ttatcgagga cctttgtaaa
    10981 ggaagaagag aaagaaactt cgactgcatt gaaattatct accggtacag acagtgtgat
    11041 catcggtaaa ttatttttta atctgatact cacatttgct ctcaacctgc ttatacttat
    11101 tctttttatt ttaattacgg aatatcagat aaaaaatctg tcgggatttt tagttgtaat
    11161 tgtgttagga aatctgggtc ttgtatcagc atctacaatt atagcagcga taatttccaa
    11221 agcaaattca aagggcactt tatatcccgt actgtctttt ccaattttgt tgccgctatt
    11281 gatcactgtt ataaatgcaa ccaaactggc aagtcttgga gtcgaaactg ataaattatt
    11341 cggagaaatt cagatactgg tgtcttattt tgtcgttgta actgttactt cacttatgct
    11401 ttttaaattt atttgggaag attagttaat tgaatggaaa ttattataaa agtaaattat
    11461 taaaatcttt aacagacaac tatttttttc aaaaagatct tcatccctaa aataaaatat
    11521 gaataaattt attacatacc tgatatttat aataatcagc gcagtgataa tactcggact
    11581 ggcattacct attcctgaca taccggctct cggtgataaa gcaagagtac tttatttcca
    11641 tgtgccaatg tcatggatat cagtacttgc tttttttatg tcaatgtggt attcaatcag
    11701 atatctgaaa acacgcgata ttaattatga tataaaatct tattcaagtg cgcagcttgg
    11761 atttttattt tgtatactag cgactgctac aggttcactt tgggcaaagt tcagctgggg
    11821 ttctttctgg aactgggatc cgaggcagac ttcaatattt attttattgc tgatatacgg
    11881 agcatatttt gctttaagat cagccattga atctgaagat cagcgcgcaa aactttcatc
    11941 tgtctatgcg atcatcgcag ccgtaacagt cccgttcttt gtatttatta tgccgaggat
    12001 agttgagagt cttcatcctg atcctatcat taatacgcag ggaaaaatta atatggacag
    12061 ttctatgctg acaatttttc tttcatcttt ggttggattt acaatgttct atctgtggat
    12121 gctgaatctg aagattcgaa tagaaaaatt gatttataaa aaattaagct taattgaata
    12181 aatttatgac atcactgtac gattttctgg aaacaaattc tttatatata gtaatgctga
    12241 tcgtccttat aatatggatc ggcatttttg gatatatgag atcgctggat aaaaaggtaa
    12301 agaaacttga gaataatgaa taagatctag aaagttttaa aagattttaa tttggaattt
    12361 gagccgatgt gggtgtttga aaaagtttgc gaaaattaaa tgtattttaa tattataact
    12421 ttgaagactt tttcaccacc aacttgtgat ctgaagcacg tcggcattgt agttgttgac
    12481 caaaaagatt atgagaatga tacaataaat tcaagtgcag cacttaaact tattggatca
    12541 acatcaacgg agaaaataaa ttctcaaact aaaaattcta taagactttg aagaaactgt
    12601 tagtatttaa aaaattaaga atcaattata ataatcatct tcaaagccgc caaattctga
    12661 tgcatcttta gatcctgaaa acatacttcc taccatatct ttatatttta tcactgagtc
    12721 aatttcatcc tttgcaattt tggaattctg atgaagtccg tcaagtacaa attccataag
    12781 cgatgcaagc tcctgatcat tttcaaaaaa ctgaggatat ttccttacaa aaatttcaag
    12841 accatctact ttttttaatt cagcaaagta atttttaaaa ctcatatcat ctttaatatt
    12901 tacacttccg ccgaactgaa accattccgt aatttcaata taagggtctc tttgcggttt
    12961 ctcctgtgca tttttcttta atctcaatga cggatccgga aaatactttt tgaatatttc
    13021 tcttacagat ctcgagataa gtgcctttgc gactttaagt gaaccttcct gttcaccttc
    13081 aaagaccagt tccactttac cggtaatacc cggtaatata gaaagtacat cacacattct
    13141 cggcattata atatcttcag aattaataat ggatctcctc tcagcattgc tgatcagatt
    13201 ttccatagct gaaattgtga gtctggcact tactccggat ttctgatcta cgaactcgct
    13261 ggctcttgcc tgcatcgcag tcatttctat gatctctctg aagtagtaag gaatgtgaat
    13321 attcttttta tcatttctat ctgtccatga gtttgactcg gtaattttaa ttccgtcttc
    13381 cagaactttc ggataatgtg ttatgatctg agaatctatt ctgtctttaa gaggtgtgat
    13441 tatatttcct ctgttagtat aatcttcagg atttgcagta aagatcataa gtatatccag
    13501 cggaattctg atattaaatc cacgtatctg aatatctttt tcctgaagaa tattaagaag
    13561 accgacttgt atcctcggct gaagatccgg cagttcatta attacaaaaa tacctctgtt
    13621 tgtccggggg ataataccaa agtgaattgc accttcatgt gaataatgga gtctctgatt
    13681 cgctgctttt attggatcaa tgtcaccgat cagatcagca atgtttacat caggtgttgc
    13741 aagtttttca ctgtagcgct gctcacggga tatccattca atctccgttt catcaccctg
    13801 ggaatttatc atatcgacag agtattttga aattggctga aatggattat catttatttc
    13861 cgatcctttt actatcggaa tatattcatc aagcagttcc gggatcatcc tcgcaatttt
    13921 tgtctttgcc tgccctctga gtccaagtaa tattatgtcg tgttttgata acaacgaatt
    13981 catcagagaa gggatcacgc tgttttcata tccaattatt cctttgaaaa atgttccttt
    14041 ggtttcaatc tttttgatca gattttttct gatctcatct tttacagtta atactttata
    14101 accggatttt tttaattgtc cgaaagtttt tatttctttt tgattcatta aattttaaat
    14161 gggtagttat taattaattg aagcaggtca gaattttttg gaactgaata ttatccgctg
    14221 aattgaatat atgaattcag catttcaatt ttttcttcct tctgactcat gtcataagtc
    14281 atcagatccc gaattgaaat cattcctttt aaatcctgcc cgtctataac aggcatgtga
    14341 cggatctttt cctgtttcat tatctgcata cagtattccg gagtatcgtg tgattctatg
    14401 acaattactt tttttgtcat tacatcatct acagaagtat taaatagatc aatttctttt
    14461 gcaatacatt ttctcaaaag atctctctct gagaaaatac caatcagttt aagcttttca
    14521 gactgctcga gtaccggcac tgcgccgatg ttgtgaagcc ccatgaattt ggctacatca
    14581 attatcttca ttcctgattt tacaaaatgc aaaggtctgt ccttgattag atcttttaaa
    14641 gttttcattt tttacccccg gttagttttt ttgaaaaata tgcaataaat tttcaataag
    14701 caatattatc attttattat ttatttaact caaagcaatt attttcaatc ttaatcaaag
    14761 ttgataaccg gacttaaagt aaattttcaa tctgtaaata aatacagatc taattttatt
    14821 ttaagaatta aaatgtgaaa ctgaattgta ctacaggtta gctgaataga ttacttgatt
    14881 ttgattactt cagatcattc ttcttcaatt ccatcagata cttcgaaatc aaattttcat
    14941 aatcttcatt aaacccttct ttatccaatc ttaaaaaatc ttccttaaga gcattatagg
    15001 aatttggtcc gcttagaatt atttcaggcg gggaatttct gctgacattt tcacccggac
    15061 gggattctct ttttggttca aaatcttttt cgcgttgaga cagacgtgca tccagaagtc
    15121 tggacaatat cctgttttgc ttttctatta atttgtcatc tacgttatat tcactcaggt
    15181 cttttacaat ctcctgcatt tctttttgta tctgatctaa atctcctaaa actttttcac
    15241 ctgaattctc ctgttctctt tttaactctt cattaagcat ctctaatgat ttctgaatct
    15301 gctgctgttc catgctgaga cgttgcattt cctgtctctg ctcttctgac atattatccg
    15361 gtttattccc cttgccgtct tttcctgatt taccgttttc acctgattca ccattcattc
    15421 ccatctttcc cgatttacca ttcatcccca tttgctgagc gattatttca ccgagtctgt
    15481 ccatcatctg tcccatattc ccggagccgg gtttgtttcc ctttccatcc ttaccatctt
    15541 tgccatcctc accgccttta ccatctttac ccattttccc aagcatgttt ccaagcatct
    15601 tggctgcgtt atcaagggac tcttttgctt tcttctggtc agatgaagca tcttgtttct
    15661 tcatttctcc aagttcttta ccggctttct ccattttgtt gtatgcgtct ccaagttctt
    15721 tcccaagttc aggcgacatt ttcattccca tttttgaagc attcataaga tcatcaatac
    15781 tttctgataa ctgtttctga agatcatttt ggtctttctg attcttttca aattcttcct
    15841 tctcatcttt atcaagttca tctgtcttat cttttaactc ctgctgtctc ctgctcatct
    15901 cttcaagttt ctttttaata tcttccaatt tctctttgag cttgttattc atatcctgag
    15961 aatccatcat ttcatttaat gcagctttca tttttttatc catattattc agctcatcca
    16021 tgatctcttc ctgtgttttc tctgaattac tcttctgaga agattttaat tcatcactgg
    16081 atttctgcat cttattctca gtgtccttct tattcatttc cttctgaatt ttctcaagct
    16141 cttcaggact tagctgatct ttcatcttat tcatttcatc tataagcttc tgaagctcac
    16201 tattgaaatc ttttgtctgc tcttttatgt ctttttgttt ttgagaaagt tcgtccatct
    16261 tattcttatc actattctgc gaattttcag tttccttttt taactgctcc tgctgctttg
    16321 caatatcttc aagcttttga gtaagctcac caaatttctg catgttctct atctttttca
    16381 ttagctccat tgccttttcc atatatttct taaatgcctc ttcatcaaat ttgaaattct
    16441 tcattgcgtc tttaagctct tcctggttat tcttcttcaa agcttctttt aacttttcaa
    16501 gcatcttctg aagttcagga gaatttattt tgttaaacat tttctgcagc tccataaact
    16561 gttccagagt cttctcaccc agcatattat tctttttcat ctcttccatg ttctgatcaa
    16621 gcttgttctg agcagagttc atattcttct gaaagttatc aagcttttgt tccatctgtt
    16681 tcttgctgtc ttcattcaga ccaagctctt cattcttctg aaattccttt ttgatgtctt
    16741 caatgttttt actcaattct tccatttgat cataagctga ctgcatttca gttttcagtt
    16801 cctttgtcat cgtctcagtc tcttttaaaa cttctgcctg agatctgtat tgaatggtct
    16861 ttatttccga cctggttgat ttaccggtat tgtcagtgac ttctgtaaaa tattctgcac
    16921 tctctcctga gcgtaaatta attcctgata cattccacac ataaggaacc tcaagtgaag
    16981 ttgcatttgg atttttgatc tcaatattta tataattaaa ttcaggggca gatccgctgc
    17041 ctgaaagaag ctttaatttt ctgtaaccca ggactaattt agaaaatccg taatcatctg
    17101 aaattctcga tcttactatt aattctctct cgccttttaa ttcgtaatta gattcagacg
    17161 gttcaataat agtaattttt ggcggctcat caggcattac ctttattgta tattgcttaa
    17221 agtttttatt cactccgccg ttcaggtctt taagaataaa tctgtaactg ctgctctgat
    17281 ctgctttgat actgcccttt gctgaagttc cgttaaattc aaatgaaacc ggatttccgt
    17341 tcagatcaat accagcatat gaaagttcag tgcttgcatt tatattaaac tctattgtac
    17401 ttccctccgg acagaatata tttccttcgt tatcaggtaa tgttttggat ggtattccgg
    17461 taaactcagg aggagtaact gtgacagtga aattttttat cacaggatat tccgaaacat
    17521 tgatagtgta ttcctcagat ctaattcctt tatattccgc aaaatatata agttcattga
    17581 tagtgttttt tattgtgcct gtaaaattat ttcccgactc tgaaattaat ttcaccggat
    17641 ctgaaagcat ctcatatccg tcagcagaaa cctgtttagt gtaaaggtta atttctttta
    17701 tattcagatc atttgaggtg gaattaatgg taactcctat tatcacgtct tcacctgctg
    17761 caatctcaat atcgccgggg atgatttcaa aagtaatgcc cagatcatta tccagaaaaa
    17821 agtaattatg attgaccagt ctttttactg caccattaag attcttaggg aagattaaaa
    17881 atgaagcggt gataaataca attattgaaa gtaaaattaa gattaatttc tttagcgatg
    17941 aaaattttat aaatgaagac aggtctttat taccggctat attgttaata ctttttatgt
    18001 cttcaaagat gagctcatct gatactgcac tgttttttct gtcggaattc ccgggcatta
    18061 ctaaagataa agaattcgaa agcttatcct ttatatcctc aaaatgatcc ccgacttttc
    18121 ttgaatatga aatgagatta aagtttccga ttattcctga actctttaaa aggaaattta
    18181 ataaaaaata agttattgtt gtgatcgaaa cagaaagaaa tccccagtat aaaaatgtcc
    18241 gtgtaaatat cccgaatctg aatacagatt ccagtacagt tattaaaaaa cctaataaca
    18301 aaaatattat gagagatgta attgtattcc ttagaaatga aaaaagcagc tctttcttat
    18361 ttacagcaga aagctttttt tcaaaaaagt tatatattga aattgtactt aaggagtttt
    18421 gcattagaat tttttatagg tctttataac cctgttaaag taataatgtt tcaggtaaat
    18481 ctcaattgat gaaaaaatca aattcagatg agcttttata taactcataa cttataaccc
    18541 atttcaacaa tctcatttcc taaagtttct atgactctgt tttgatctga tatagacat
//
LOCUS       DAORVQ010000045        18104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1279_length_18104_cov_5.557095, whole genome shotgun sequence.
ACCESSION   DAORVQ010000045 DAORVQ010000000
VERSION     DAORVQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 18104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18104
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_18104_cov_5.557095"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="PLX80_05540"
     CDS             complement(<1..1574)
                     /locus_tag="PLX80_05540"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQY20292.1"
                     /translation="MSNKYVYYFGNGKADGKADMKSLLGGKGANLAEMTNIKLPVPPG
                     FTVTTEVCNYYYKNKKKYPKELKKQVELALKKIEKDMNCKFGDNKNPLLLSVRSGARA
                     SMPGMMDTVLNLGLNDASVKGLIEKTGNERFVYDSYRRFVQMYGDVVLDLKPNDKNQI
                     DPFEVIIEKKKEQRKIKNDIDFTVDDLKELVDEFKKAIKTETGHNFPDDPMVQLWGAL
                     GAVFRSWMNDRAIVYRKLNSIPVEWGTAVNIQAMVFGNLGDTSGTGVAFTRNPSNGED
                     EFYGEYLMNAQGEDVVAGIRTPLPISDLKKAKPKIYAELEKVRKSLEKHYKDMMDIEF
                     TIQDEVLYMLQCRAGKRTGYASVKIAVDMVKEKLISDKTAVMRVDPEQLNQLLRPIFS
                     TSEKQEAIKDGKLLAIGLNAGPGAACGKVYFNSFDAVNAKARGEDVILVRIETSPEDI
                     RGMNAAEGILTSRGGMTSHAALVARQMGKVCVAGCGTLEIDYLLKTIRVFGKQHLVHE
                     GDFISIDGSTGEVIIG"
     gene            1833..3080
                     /locus_tag="PLX80_05545"
     CDS             1833..3080
                     /locus_tag="PLX80_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY20293.1"
                     /translation="MNNNISVKKHDPYEALRFPEFRNFNIARLCITIASQMQAVIVGW
                     QIYEITKDPLSLGLIGLAEAIPSICTLIFAGHITDTNDRKKIVMTSVILMTICSILLL
                     IVSTDGIEVLYSNKVLAIYSIIFLSGIGRGFSSPSFFAFVAQLVPKESLPNAITWNTS
                     TWQTGAVTGPALGGLLYGFIGAVQTYSIIVLFWIIAIILISTIKKKPIPEFVALLSLK
                     EKLTSGLKFILEKKIILGAISLDLFAVLFGGAVALLPIFAGEILFVGPEGLGMLRAAP
                     SVGAVTMALLMTRRPITGNAGRNLLIGVFGFGICIVIFGLSKSFYLSLVILCFSGAFD
                     SMSVVIRSTVIQLLTPDNMKGKVSAVNSMFIGSSNEIGAFESGVAAKLMGTVPSVIFG
                     GLMTLIIVTCVTIFSPRLRNLKL"
     gene            complement(3075..3755)
                     /locus_tag="PLX80_05550"
     CDS             complement(3075..3755)
                     /locus_tag="PLX80_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HQY20294.1"
                     /translation="MCRQFITAGKLSNLRKLIEKTYNEDIRIKLLGEIAEEEENIQEE
                     IEKELKETIKRNKDFDAILTHNVPVIAYENGEKILTKMKWGIRFSPEKKSPLIFNSRD
                     DTIQTKPFWTKTFDKNRILIPMFGFYEWQDKGGKKKVKTKITLPGRNLFMVPGLYWKN
                     KEGINEFTLITTSPSSFMKKIHSRMPVLLDDKNVFNYFTDSFEENAEKLKPSEGNMNL
                     EEDNPDNL"
     gene            3969..4802
                     /locus_tag="PLX80_05555"
     CDS             3969..4802
                     /locus_tag="PLX80_05555"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQY20295.1"
                     /translation="MIKDKILVIRFSINGNEYKSDLSVPMEISIPLDFYGDQPNTYDV
                     ERAAAKAFRSGEFIGDTNEGGGCNFEEYKIIPHCNGTHTECVGHISFERISIESVLKD
                     IFIPSILITVIPENSSDTDDTYNPQKINEDYLITEKSLREKLENADADFCKGLIIRTL
                     PNSEEKKRRRYMNNLPPFFSIEAMRYISGLEVKHLILDLPSVDRTFDEGKLSAHHIFW
                     NVKEGSHEVNVNDHSFKTITEMAFMQDKIKDGKYLLNIQIPGFKADAAPSRLFLFEIK
                     N"
     gene            4837..6096
                     /gene="kynU"
                     /locus_tag="PLX80_05560"
     CDS             4837..6096
                     /gene="kynU"
                     /locus_tag="PLX80_05560"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HQY20296.1"
                     /translation="MNFGCDIAFAKETDEKDPLRHFRDRFFIPKDSNGNDAIYLAGNS
                     LGLQPKSLRNYIEQELKDWETFAVEGHTKAKNPWLPYHEFLTRQTANLTGALESEVVN
                     MNSLTANLHLLFISFYRPEKNKHKILIESNSFPSDHYAVQSQINFHGYDVSDSLIEMK
                     PREGEDKIRTEDIEELIEKEGDEIALIWFAGVNYYSGQAFEMERIVKAGQAKNCKVGF
                     DLAHATGNLELKLHDCNADFAVWCNYKYMNGGPGAIGGAFVHERHLKNDELKKFLGWW
                     GHDKETRFKMDHKYIPIPTVESWQLSNPPILQLASLKASMDIFEEAGMSVLRKKSEHM
                     TGYMEYLLKRSENSGIQIITPAEINERGCQLSLRVKENGKELFNRLSDKGVICDWREP
                     DVIRVAPVPLYNSYVDVWKFVQILSGG"
     gene            6269..7639
                     /locus_tag="PLX80_05565"
     CDS             6269..7639
                     /locus_tag="PLX80_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY20297.1"
                     /translation="MKNITIVGAGLAGSLLSVYLAKKNLNVNVYERRPDMRLSETDGG
                     KSINLALSTRGIHALKEVGIYEDIKKISIPMYGRMIHSVDSELMFQRYGKDDSEYINA
                     VSRAELNKKLLDLADKYDNVNILFNEKCIGADFKKTEVSFYNEVTKNESTVTSDAVIA
                     ADGATSAVRLEMQKIPRFNFSQEYENYGYKELIIPADKNGGFKMEKNALHIWPRGSFM
                     LIALPNLDGSYTCTLFMAYDRSLGGKCSFEYIDSEEKLTEFFETQFKDAYDLIPDLNN
                     EFFTNPTGTLMTIKCYPWTAGENSVILGDSCHAIVPFFGQGMNAAFEDCVYMNECFEK
                     YGDDINKIFTEFQELRKENSDAIADLARENFIEMRDLVADKTFQFKKKIESELFKIYP
                     DTFIPKYSMVTFMRIPYSVALKRGKIQEEILSILSKNINDIKDLDLKKADEIIKDKLQ
                     KLDLKN"
     gene            8227..9648
                     /locus_tag="PLX80_05570"
     CDS             8227..9648
                     /locus_tag="PLX80_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20298.1"
                     /translation="MFREFVDNKIKKETEQFADAKSITKDLINKSEVSDFTINYLLFY
                     LTDDIKAEELPAMINKANKLYFNYALRPKWTLINFLFNNFESRSPAEILKKLMLFPFY
                     KYYTDAVSDFINDNSMIFITKGEIENIIDRTNQAIHSKLTVEITGQKIKNVFLQLFIL
                     NDFYEEKYNLESAIPFSFIKIFLSDKNYSDILSKFDVLGNKQDHENIQLKDIIKVLTG
                     KFAENEKTDSQKTESEKPIPITVEAVPSGISITEKNIDSQNSSQQEFINIKDKDVDVN
                     KPGSITVKNINEIADKNSDKNINKNTKVNESDFSIKKITQEPTETSLKSSEEKQNDND
                     EGFVIKEKTAPPKPVLSDDDIESTESNNDTKGKPIEIPLVEMNYVSTDYNNESEKVIF
                     KHKNIDFKELFSEKELDSILNRVYDSNLMARQRSFEKLNNFKTWFEAFNHLKEIFKNN
                     KVDIYHKDVIRYVDALNEHFQNS"
     gene            9675..10706
                     /gene="obgE"
                     /locus_tag="PLX80_05575"
     CDS             9675..10706
                     /gene="obgE"
                     /locus_tag="PLX80_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQY20299.1"
                     /translation="MFLDSAKIFIKSGNGGNGCVSFRREKYVPKGGPNGGDGGKGGDI
                     IFKASSQLTTLIDFRYQAHHRAKRGDHGQGGLKTGKNGESEIILVPCGSIVKDAVTGE
                     VLAELLEDDEEVILLKGGKGGKGNNHFKTSTNRAPRIAQDGEKGEEKDIVIELKLIAD
                     VGLVGFPNAGKSTLISTISAARPKIADYPFTTLVPNLGIVRYKEYDSFVVADIPGIIE
                     GASDGKGLGIQFLKHIERTGILLILLDSTKLEKAKTKKDLLVDYTILMKELKNFEADL
                     TAKPRIICFTKIDSITDELKAKLVKLKTKEPKILISSFTDENIDELKDLMWDMISKLK
                     EEEEEVKSE"
     gene            10750..11520
                     /gene="surE"
                     /locus_tag="PLX80_05580"
     CDS             10750..11520
                     /gene="surE"
                     /locus_tag="PLX80_05580"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQY20300.1"
                     /translation="MKKPTILISNDDGIESEGIRALWREIRKFAEVIVVAPHTQQSGV
                     GHFITFSSPIRARKEIIDKNFYGYAVEGSPADSVKLAVRNLLKGKKIDLLLSGINNGA
                     NTAINIIYSGTVSAATEGTILGIPSIALSQASFTSNDFSVAAKFASHISKIVLKKGLP
                     HGTLLNVNIPAVPKSEIKGIIITRQGKSYWDDKYEARTDPNNREYYWLTGKMASLDKT
                     IEFDQKAIEENYISITPIQYDLTDYKMLEELEDWKLKL"
     gene            complement(11607..12890)
                     /locus_tag="PLX80_05585"
     CDS             complement(11607..12890)
                     /locus_tag="PLX80_05585"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HQY20301.1"
                     /translation="MSKLMKERLASQLPALREEAKSIAKNHGSEVIGQVAIEQLYGGM
                     RGIKGLICDTSEVGLQTGLVIRGIPILELTDRSPEEIFYLLVIGKLPNETELKDLQDD
                     LHERSDVPSYVWDVLNSMPEDSHPMVMFSTAINVMEKESEFRNEYNNGLGKDKLWEPM
                     YEDCLNIIARVPQIAAFIYRKKFKKGDMIPPDKNLDWAENFAKMLGVDDKEGTFAKLL
                     RLYLVLHSDHEGGNVSAFTCLVISSALSDAYYSITGALNGLAGPLHGLANQNCLKFIL
                     KLKEDIGHSPTDDEVRKYCQDLLDRGGLVIGYGHAVLRITDPRFTAFHDFGKKHLPDD
                     EVFKIVAQLFEIVPDLLKEQGKAKDPWPNVDAASGSMLYHYGMKEYDFYTVLFGVSRI
                     LGFSSQMIIARAMGSSLIRPKSVTTDWIKKEIASK"
     gene            13209..13886
                     /locus_tag="PLX80_05590"
     CDS             13209..13886
                     /locus_tag="PLX80_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQY20302.1"
                     /translation="MSEIIYHGHSFLEIKTTDLTIYIDPFITGNTLCDIKTEDAKCDY
                     IFLTHGHNDHVGDTIEIARGKNITVIGVVELTDYLESKGISVHGMNIGGAYTFPFGKV
                     KLTIAHHSSSIMNGPYAGEPAGLILNIEGKTIYHSGDTALFYDMKLIGEMNNIDYAFL
                     PIGDNFTMGIDDAVKAAGFLNAKNIIPIHYNTFDLIKANEKEFKRKIESIGKICTVMN
                     PGDSVNF"
     gene            13938..14789
                     /locus_tag="PLX80_05595"
     CDS             13938..14789
                     /locus_tag="PLX80_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY20303.1"
                     /translation="MTKIISISNQKGGVGKTTTAINLSSAIAAYGKKVLMIDTDPQAN
                     ATSGLGLDNSIEGNSTYEIFISDKKVKDCIKDTQVQNLKIISANINLVAAELELVDLP
                     HRESILKNKLKEFLAENIEDFDFILVDCPPSLGLITVNALTAADSLLIPVQCEYYALE
                     GLSQLLNTIKNVKNIFNPDLNIEGYLLTMFDSRLKLSNQIESELRKFFGEKVFTTKIF
                     RNVKIGEAPSYGKPIIVYDPASTGALNYMDLGKEFLLRNGFETESEKNSDNSNNSEIN
                     EIKVNET"
     gene            14801..15685
                     /locus_tag="PLX80_05600"
     CDS             14801..15685
                     /locus_tag="PLX80_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQY20304.1"
                     /translation="MENKTSALGKGLSAIFDEKKIDVSFVEESAKKNSTVLNVSEIRV
                     NPAQPREDFDEEKLKELSDSIRQKGLIQPVTVKKTENGYVLVSGERRLRAVKLIGMEK
                     IPVYFMENNNGSDEDMLELALIENLQREDLNPMELSDSYFKLIELHNLTQEKIAEKVS
                     KNRSTVANYLRLQRLPIEIKISLRKNEITEAHARLLLRVDNEDDQITLWKRIVNENIT
                     VKNLQEITKQDVKKPKAKKPKLGEIYDPYFQSVEDKLRNFLGTKVSVKRKSKYSGEII
                     IEYFSNDDLERIIEKCSD"
     gene            15720..16100
                     /locus_tag="PLX80_05605"
     CDS             15720..16100
                     /locus_tag="PLX80_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009991672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY20305.1"
                     /translation="MKSILISDKAAPPVGPYSQAVGIGDLIYTSGQIPFDVDGNLVSD
                     NIEGQTEKVLENLKSILEDNGSSLDNAVKTMVFLNDMNNFAKMNEVYSKYFVNSPPAR
                     STVEVSRLPKDVLVEIEIIAFKNK"
     gene            16110..16559
                     /locus_tag="PLX80_05610"
     CDS             16110..16559
                     /locus_tag="PLX80_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQY20306.1"
                     /translation="MNPLHDRLCPGKLKFTVGIVIKSDLRTVWNNVCKAEQVRKYFTT
                     DARRDLDSSGDVLWIWGNEAALIKVVEILPEDKLVFEWNGSNVDYRIRTEFIFKKEND
                     KVTLKIKEKGWEENAKGMKSAFLNCSGWTEFLSALKVFTEYQISYLK"
     gene            complement(16608..16868)
                     /gene="rpsT"
                     /locus_tag="PLX80_05615"
     CDS             complement(16608..16868)
                     /gene="rpsT"
                     /locus_tag="PLX80_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQY20307.1"
                     /translation="MPLRHKSAQKRARQTVKKTERNKKYKALIKGSVKRVMSKEEKSE
                     AAAEFKKTSQVLDRAAVKGIIHKNKAANQKSKIAKHINKLEK"
     gene            complement(16978..17631)
                     /locus_tag="PLX80_05620"
     CDS             complement(16978..17631)
                     /locus_tag="PLX80_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HQY20308.1"
                     /translation="MGLFRKNNLDINDESLLPKHRVEALCDGIFAIVMTIIILDLKTP
                     ENIPKDLIHFELPDMFFNLLPAIEAYVLSFLVLGVFWLRHQIQFKFITTVDRSIIVLN
                     ILFLMLTGFIPFTVGVLMKYPRSEFTFLIYALNLILISIIMIIHLNYIFSKSNVFTDS
                     ISEEDYTKTKILSYIPVLIFVISIFVSFYSVRMALFIIYLDPLFFTIYRSINYFKRE"
     gene            17703..>18104
                     /locus_tag="PLX80_05625"
     CDS             17703..>18104
                     /locus_tag="PLX80_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQY20309.1"
                     /translation="MGNKITLNTLNSNSNNNSNINKNILNVPDDPIIPYIDGDGIGPE
                     IWRTARHVFDSAVEKAYNGKRKVEWMEVLAGGKSFEKTKSWLPDETLDAFREYLVGIK
                     GPLTTPIGKGIRSINVALRQKLDLYVCLRPVK"
BASE COUNT         6274 a         2871 c         3167 g         5792 t
ORIGIN      
        1 ttacctataa ttacttcacc ggtagaaccg tcaatagaaa taaaatcacc ttcatgtact
       61 aaatgctgtt taccgaaaac acgaattgtc ttcaataaat aatcaatctc aagcgtacca
      121 catcctgcca cacagacttt tcccatttgc ctggctacaa gagcagcatg tgaagtcatt
      181 cctcctcttg aagttaaaat tccttctgca gcattcattc ctcttatatc ttcgggagaa
      241 gtttcaattc tcactaaaat tacatcttca cctcttgctt ttgcatttac ggcatcaaag
      301 gaattaaaat aaacttttcc gcaagcagca ccgggtcctg catttaatcc aatagcaagg
      361 agtttaccat ctttgattgc ttcctgtttt tcagatgtac taaagatagg tctgagtaat
      421 tggttaagct gttcggggtc aacacgcatc acagcagttt tatcagaaat aagtttttct
      481 tttaccatat ctactgcgat cttaactgaa gcataacctg ttctttttcc agcacggcat
      541 tgaagcatat acaaaacttc atcctgaatt gtaaactcga tgtccatcat atctttgtaa
      601 tgcttttcca gactctttct tactttttca agctctgcat aaatttttgg ttttgctttt
      661 tttagatcgg aaataggcag cggagttctg atacctgcta caacatcttc accctgtgcg
      721 ttcataagat attcaccata aaattcatct tcgccatttg aaggatttct cgtaaatgct
      781 acaccggttc cggatgtatc accaagattt ccgaatacca tagcctgaat attcacagca
      841 gttccccatt cgacaggaat gctgtttaat tttctgtaaa ctatagccct gtcattcatc
      901 catgatctga atacagctcc aagtgcaccc caaagctgaa ccatcggatc atcaggaaaa
      961 ttatgtcctg tctcggtttt tattgctttc ttaaattcgt ctacaagttc tttcaggtca
     1021 tcaactgtaa aatcaatatc attttttatt tttctctgct ctttcttttt ttcaataatg
     1081 acttcaaacg ggtcaatctg atttttgtca tttggtttaa gatcaagtac aacgtcccca
     1141 tacatctgaa caaatcttct gtatgaatca taaacaaatc tttcattgcc tgttttttct
     1201 ataagacctt ttactgatgc atcattgagt ccaagattta atacagtgtc catcatacca
     1261 ggcatagaag ctctggcacc agatctgaca cttaaaagta aaggattttt gttatcccca
     1321 aatttacagt tcatgtcttt ttcaatcttc tttaaagcga gctcaacctg tttctttaat
     1381 tcctttggat attttttttt gttcttataa taataattac aaacttctgt agtgacagta
     1441 aagcccgggg ggactggcag tttaatgttt gtcatctccg caaggttagc gccttttccg
     1501 cctaacaaag atttcatatc cgcttttcca tctgctttac catttccaaa atagtaaacg
     1561 tatttgtttg acatgattaa aaaatttaag tgagaaatgt gttaaatatc tagcaatata
     1621 atcatttata aatttttatt ttattctcaa ttaaatacat tttcaataat tcagtttgtt
     1681 acaatcttcg gatatttaca aacctaaata tcaataacat tattttaaag cattctaaaa
     1741 agtattatat tgactaatta aaaatttgtt taagaactga atcacatgat taatattcat
     1801 ttttgtttaa attatttaca aagtgaaatt ttttgaataa taatatatct gttaaaaaac
     1861 atgatcctta tgaggcatta agatttccgg agttcagaaa ttttaacatt gccagactat
     1921 gtattactat tgcgtctcag atgcaggcag taatagtggg ctggcagata tatgagatca
     1981 ctaaagatcc tttgtcttta ggactgatcg gtcttgcaga agcaatccct tctatctgta
     2041 ctttaatatt tgcaggtcat ataactgata ctaatgatag aaaaaaaatc gtaatgactt
     2101 cagtaattct tatgacaatt tgctcaatcc tccttcttat tgtatcaaca gacggaattg
     2161 aagtattata ctcaaacaaa gtattagcaa tttacagcat aatattttta agcggaattg
     2221 gcagaggatt ttcatcacct tcattttttg catttgttgc tcagcttgta cccaaagaaa
     2281 gtttaccaaa tgcaataaca tggaacacat cgacctggca gacgggagca gtcaccggtc
     2341 ctgcacttgg aggattgctg tacggattta ttggtgctgt gcagacttat tcgataatcg
     2401 tcttattctg gatcattgca atcatactta tctcaacaat taaaaagaaa cctatacctg
     2461 agtttgttgc tcttttatct ctgaaggaaa aacttacatc cggtttgaaa tttattttag
     2521 aaaagaaaat cattttaggt gcaatatcat tagatctgtt tgcggtatta tttggaggag
     2581 cggtggcatt gcttccgata tttgcgggtg aaattttatt tgtcggacct gaaggacttg
     2641 gaatgctgag agcagcaccg tcagtaggag cagttactat ggctcttctt atgacaagga
     2701 gaccgatcac aggtaatgcc ggcaggaatt tactgatcgg agtttttgga tttggaattt
     2761 gtattgtaat tttcggatta tcaaaaagtt tctatctgtc gctggtaatc ctttgcttca
     2821 gcggtgcatt tgacagtatg agtgttgtta tcagaagcac tgtaattcaa ctgctaacgc
     2881 ctgataatat gaaaggtaaa gtatctgctg tcaatagtat gtttattggt tcatcaaatg
     2941 agattggagc atttgaatca ggagtagctg cgaaacttat gggaactgtc ccgtctgtaa
     3001 tttttggagg actgatgaca ctgatcattg ttacatgtgt gacaatattt tcaccacggc
     3061 tcagaaatct caaactataa attgtcagga ttgtcttctt caagattcat attgccttca
     3121 gatggtttca gcttctcagc attttcttca aaagaatcag taaaataatt aaatacattt
     3181 ttatcatcga gtaatactgg catgcggctg tgaatttttt tcatgaaact gcttggagaa
     3241 gtggtgatca atgtgaattc atttatacct tctttatttt tccaatacaa accgggtacc
     3301 ataaataaat ttctgcctgg caaagtaatt ttagttttca cttttttctt tccgccttta
     3361 tcctgccatt catagaatcc aaacattggt atcagaattc ggttcttgtc aaaagtcttt
     3421 gtccagaaag gttttgtctg aatagtatca tctcttgaat tgaagatcag cggggatttt
     3481 ttttcgggac tgaaacgaat tccccatttc atttttgtaa gaattttctc accattttca
     3541 taagcaataa caggtacatt gtgagtcagt attgcatcaa aatctttatt tcgtttaatt
     3601 gtctctttca gttccttttc aatttcttcc tgaatatttt cttcttcttc agctatttca
     3661 cctaaaagtt ttatccttat atcttcattg taagtcttct caattaattt tctgaggtta
     3721 gaaagtttac ctgctgtaat gaattgtctg cacataaaaa atttattttt tgtaaaataa
     3781 aatttcttat tatttattga aatgagaaaa ttgctttcat ttaaagcagt tgcatttgaa
     3841 acgagtttca atatcaattg catatccaaa caggagtttg gatacgagcg gtttattaaa
     3901 attacgataa tattaatctg aattcagcgg atgaatgtga attgaattat ttaccaaata
     3961 aaaatacttt gataaaagat aaaattttgg tgattcgatt ttcaataaat ggtaatgagt
     4021 ataaaagtga tctgtcagtt ccgatggaga tttccattcc attggatttt tatggagatc
     4081 agccaaatac atatgatgta gaaagagctg cagcaaaagc ttttaggtct ggagaattta
     4141 ttggagatac caatgaaggt ggcggatgta attttgaaga atataaaatt atcccgcatt
     4201 gtaacggaac tcatactgaa tgtgtaggtc atatttcgtt tgaaagaatt tcaattgaaa
     4261 gtgtattaaa agatattttc ataccgtcaa ttttaatcac tgttatacct gaaaattcat
     4321 cagatactga tgatacttac aacccgcaaa aaattaatga agattatctg ataactgaaa
     4381 aaagtctgag agaaaaactt gaaaatgctg atgcagattt ttgtaaaggg ttgattatca
     4441 gaacgcttcc aaacagtgaa gaaaaaaaga gaaggagata catgaataat cttcctccat
     4501 ttttttccat tgaagctatg agatatatat ccggattaga agtaaagcat ttgatacttg
     4561 atctgccttc tgttgacagg acctttgatg aggggaaatt gtcagctcat catatttttt
     4621 ggaatgtaaa ggaaggaagt catgaagtta atgtaaatga tcattcattt aaaacaatta
     4681 cagaaatggc atttatgcag gataagataa aagacggaaa atatttacta aatatacaga
     4741 tacccggatt caaagctgat gctgcaccca gcagattgtt tttatttgaa ataaaaaatt
     4801 aactgtgaaa aaattattaa ttcaaattat agttaaatga attttggctg tgatatagca
     4861 tttgcaaaag aaacagatga aaaagatcct ctcaggcatt tcagggatag attttttatt
     4921 ccaaaagatt cgaatggaaa cgatgcaatt tatcttgcag gaaattcact tggactgcag
     4981 ccaaagtctt taagaaatta tattgagcag gaactgaaag actgggaaac ttttgctgtc
     5041 gaaggtcata ccaaagcaaa aaatccgtgg ctcccatatc atgaatttct gaccagacaa
     5101 actgcaaatc tcaccggagc tttagagtct gaagttgtga atatgaattc tcttactgcg
     5161 aatcttcatt tactttttat ttcattttac agacctgaaa aaaataaaca taaaattctt
     5221 attgaatcaa attcatttcc gtcagatcat tatgcagttc aatcacagat aaattttcat
     5281 ggatatgatg tttcggattc acttatcgaa atgaagccaa gggaaggtga agataaaatc
     5341 aggacagaag acattgaaga gttgatagaa aaagaaggtg atgagatcgc gctgatatgg
     5401 tttgccggtg tgaattatta ttcagggcag gcatttgaaa tggaaagaat tgtcaaagcc
     5461 ggacaagcaa agaattgcaa agtcggattt gatctcgcac atgcaacagg aaatctggaa
     5521 ctgaaactac acgactgtaa tgcagacttt gcggtatggt gtaattataa atacatgaac
     5581 ggaggaccgg gtgcaattgg tggagcattt gtacatgaga gacatctgaa aaatgatgag
     5641 cttaaaaaat ttctgggctg gtggggtcat gataaagaaa caagatttaa gatggatcat
     5701 aaatatattc caattccaac tgtcgaaagc tggcagctca gcaatcctcc tatacttcag
     5761 ctcgcatctc tgaaagcttc gatggatata tttgaagaag cgggaatgag tgtactcaga
     5821 aaaaaaagtg aacatatgac aggctatatg gaatatctat taaagagaag tgaaaattca
     5881 ggcatacaaa taattacacc agcagaaatt aatgagagag gctgccagct ttcgctgcga
     5941 gttaaagaaa atggaaaaga attgtttaac agactttcgg ataaaggagt gatatgtgac
     6001 tggcgagagc ctgatgtgat cagagtagca cctgtacctt tgtataacag ttatgtggat
     6061 gtgtggaagt ttgtgcagat actgagcggt ggttgatgta acgtgatttt tatttttggt
     6121 aattcagata aaacctgact ggtttttttt ggaaaaaatt ttgtaaatta aggtgggagg
     6181 aaaaccagtc aggtttctca atcttcaaaa ccaaactcaa attaaaaata aaacctgaac
     6241 ggtttcaaaa accaaacaat tacaatcaat gaaaaatatt accattgtcg gtgcaggtct
     6301 cgcaggatct ctcctttctg tctatctcgc aaaaaaaaat ctgaacgtta acgtctacga
     6361 aagaagaccc gatatgcggc tctctgaaac tgacggagga aagtccatta acctcgctct
     6421 gtccacacgc ggaattcacg ctctgaagga agtcggcata tatgaagaca ttaaaaaaat
     6481 ttcaattcca atgtatggaa gaatgatcca ttcagtcgac agcgaactta tgttccagag
     6541 atacggcaaa gacgattccg aatatattaa cgcagtctca agagccgaac tcaataaaaa
     6601 acttctcgac cttgctgata aatatgataa tgtaaatatc ttatttaatg aaaaatgtat
     6661 tggtgctgat tttaaaaaga ccgaagtatc attttataat gaagttacaa aaaatgaatc
     6721 cactgtaacc tctgatgcag taatagctgc tgacggtgca acttctgccg tgcgtttgga
     6781 aatgcagaag ataccaagat ttaatttttc acaggaatat gaaaattacg gatataagga
     6841 gctcatcatt cctgctgata aaaacggggg attcaaaatg gaaaagaatg ctctgcacat
     6901 ctggcctaga ggttccttta tgctgatagc actgccgaat cttgacggaa gttatacctg
     6961 cacactgttc atggcttatg atcggtccct cggcggaaag tgcagttttg aatatatcga
     7021 ctctgaagaa aagcttacag aattttttga aactcaattc aaagatgcat acgatctgat
     7081 ccccgatctc aacaatgaat tttttactaa tcccacaggc acactcatga caataaaatg
     7141 ctatccatgg actgcgggtg aaaattccgt aatacttgga gattcatgtc atgcaattgt
     7201 tccattcttt ggacagggca tgaatgctgc atttgaagac tgtgtgtata tgaatgaatg
     7261 ttttgaaaaa tacggtgatg atataaataa aatttttact gaatttcaag agctccgtaa
     7321 agaaaactct gatgcaattg cagatcttgc aagggaaaat tttattgaaa tgcgtgacct
     7381 cgtagctgac aaaacctttc agtttaaaaa gaagatcgaa tcagaattat ttaagatata
     7441 tcccgatact tttattccga aatattctat ggtaactttt atgaggatac cttattccgt
     7501 agctcttaaa agagggaaaa ttcaggaaga gatactttcc attctaagta aaaatatcaa
     7561 cgacattaaa gatcttgatc taaagaaagc agatgaaatt atcaaagata aacttcaaaa
     7621 gcttgatctg aaaaattaag attttttgat ttcaatctat aacctggttt taaacagatt
     7681 atttgcttag atgcttttgt gatttttatt cctgatttct aacattttac atttcaaagt
     7741 ctgtccgact ctttgtcaat taattgaaca caaagattat aacctatatt tttattttcg
     7801 taatattctc actgaatata tttgggttgt tacgaacccg tttaattgtt tatagacgcg
     7861 cagcggaaga cagacgttaa aaaaacgaag aatcatttag agattaaact ttttttagga
     7921 atgattcgat agcatatgta ggtttttggg aactggagtt gaaaccgttt taacggttaa
     7981 tagcagccgc ccgatttgtt ataaatcaat attaagtagt agatcgggcg gtattatttc
     8041 atatataact gatcaaaaat acaggtgttt attttcgaaa ttgaatttta gtctgtaagg
     8101 ttaattgatc tcgacagtta ttctgtaaat attttaaatt taaaatttat aatataaaat
     8161 taatcattac ctttaaataa aaatctaaat aaactaaatt tgatgaaaca ataataaaaa
     8221 tacttaatgt ttcgggagtt tgtagataat aaaataaaaa aagagactga gcagtttgca
     8281 gatgctaaat caattacaaa agatctgatc aataaaagtg aagtttccga ttttacaatt
     8341 aattatttac tattttattt aacagacgat atcaaagccg aggaattacc ggcaatgatc
     8401 aataaagcaa ataagctcta ttttaattat gctctccgtc caaagtggac attgataaat
     8461 ttccttttca ataattttga atccagatct ccggcagaaa ttttaaaaaa acttatgctt
     8521 tttccttttt ataaatatta tacagatgct gtttctgatt ttataaatga caactcaatg
     8581 atattcatta caaaaggtga aattgaaaat attattgaca ggacaaatca ggcgattcat
     8641 agtaaactaa ctgttgaaat taccggtcag aagataaaga atgtattcct tcagcttttt
     8701 atacttaatg atttttatga agaaaagtat aatcttgaat ctgctatacc attttcattt
     8761 attaaaatat ttctttcaga taaaaattat tccgacatac tctcaaaatt tgatgtcctt
     8821 ggaaataaac aggatcacga aaatattcag ctcaaagaca ttataaaagt cttaactgga
     8881 aagtttgcgg aaaatgaaaa aaccgattct caaaaaactg aatcggaaaa acctattcca
     8941 ataacagttg aggcagtacc ttcaggcatt tcaatcactg aaaaaaatat tgattcccaa
     9001 aatagttcac aacaggaatt tataaacatt aaagataaag atgttgatgt aaataaaccc
     9061 ggatctatta ctgttaaaaa tataaatgag attgctgata aaaattcaga taagaacata
     9121 aataaaaata ctaaagtaaa tgaatcagat tttagtatta aaaaaatcac ccaagaaccg
     9181 actgagactt ccttaaaaag ctcagaagaa aaacaaaatg ataatgatga aggttttgta
     9241 ataaaagaaa aaactgcacc tccaaaacct gtgctgtcag atgatgatat agaatctacg
     9301 gaatctaata atgatacaaa aggaaagcca attgagatac ctttagtgga aatgaattat
     9361 gtcagtactg attataataa tgaatcagaa aaagtaattt ttaaacataa gaatattgac
     9421 ttcaaagaat tattcagtga gaaagagctc gattcaattc tcaacagagt ttacgattcc
     9481 aatcttatgg caagacaaag atcatttgaa aaactaaata attttaaaac ttggtttgaa
     9541 gcatttaacc atctgaaaga aatttttaaa aataataaag tggatattta ccacaaagac
     9601 gtgataagat atgtcgatgc attgaatgaa cactttcaga attcataaat atatcaaaag
     9661 aataaggagt aaatttgttt ttagacagtg caaaaatttt tataaaatca ggaaatggcg
     9721 gtaacggatg tgtaagcttc agacgtgaaa aatatgttcc aaaaggcggt ccaaacggag
     9781 gcgacggtgg caaaggcggt gacattattt ttaaagcttc ctctcagctt acgactttaa
     9841 ttgacttcag ataccaggct catcacagag caaagcgggg agatcacgga caaggaggac
     9901 tcaagacagg aaagaatggt gaaagtgaga ttattcttgt accttgcggc agcattgtaa
     9961 aagatgctgt gaccggtgaa gtacttgcgg aattattaga agacgatgaa gaagtaattt
    10021 tactaaaagg cggcaaagga ggtaaaggaa ataatcattt taaaacttct accaaccgcg
    10081 cgccgaggat cgctcaggat ggcgagaaag gtgaagagaa agatattgtc atcgaactaa
    10141 agctgatcgc tgatgtaggt cttgtcggat ttccgaatgc aggcaagtca actctgatat
    10201 cgactatctc agcagctcgt ccgaagatag ctgattatcc gttcactact ctcgttccaa
    10261 atcttggaat tgtaagatat aaagaatatg attcgtttgt tgtagctgac atacccggta
    10321 taattgaagg agcttcagat ggcaaaggtc ttggcattca atttctaaag catatcgaaa
    10381 ggacagggat tttactgatt cttctcgatt ccactaaact cgaaaaagca aaaacgaaaa
    10441 aagacctttt ggtagactac acaattctta tgaaagagtt aaagaatttc gaagctgatc
    10501 ttactgcaaa gccgaggata atctgtttta caaaaattga ttcgattaca gatgagttaa
    10561 aagctaaact tgtaaagtta aaaacaaaag aaccgaaaat tctgatctct tcttttacag
    10621 atgaaaatat tgatgagctg aaagatttaa tgtgggatat gatcagtaag cttaaagagg
    10681 aagaagaaga agtaaaatcg gaataaacaa tttatcaatt cgaaattaat ttcagtaaat
    10741 ttaatattaa tgaaaaaacc aactatactt atttcaaatg acgacggaat tgagtcagag
    10801 ggaataagag cgctgtggcg tgaaattaga aaattcgctg aagtaatagt agtagctccg
    10861 catacccagc aaagtggcgt cggacatttt ataacttttt caagtccgat aagagcccgt
    10921 aaggaaatta ttgataaaaa tttttacgga tatgctgtgg aaggttcgcc tgcggattca
    10981 gtaaaacttg ctgtgagaaa tttactcaaa ggtaaaaaga tcgacctgct tttatctgga
    11041 attaataacg gagcaaatac tgctatcaat ataatttatt ccggtactgt atcagccgct
    11101 actgaaggaa cgatactggg tattccatct attgccttat ctcaggcaag ttttaccagt
    11161 aatgattttt ccgtagcagc taaatttgca tctcacattt ctaagattgt attgaaaaaa
    11221 ggtctacctc acggcacact attaaatgta aatattcctg cagttcctaa aagtgaaatc
    11281 aaaggtatca tcattacaag acagggaaag tcatactggg atgacaaata tgaggcgaga
    11341 actgacccaa ataatcggga atactattgg cttacaggaa aaatggctag tctagataag
    11401 acaattgaat tcgatcagaa agcaatagaa gaaaattaca tttcaataac accaatacag
    11461 tatgacctta ctgattataa aatgcttgaa gaactagaag actggaaatt gaaattgtaa
    11521 taataatatt caacgaattt tgatataaaa aaaaggggtc gatcataaat caaccccttt
    11581 ttctattttc taaatatcct taattactat tttgaagcaa tttctttttt aatccaatcg
    11641 gtagttacag atttcggtct tattaatgat gatcccatag ctcttgcgat tatcatttgt
    11701 gatgagaatc cgagaattct cgaaactcca aaaagcactg tatagaaatc atattctttc
    11761 atcccataat gatacagcat agatccgctt gcggcatcta cattcggcca tggatctttt
    11821 gctttcccct gttctttcag aaggtcaggt acaatttcaa ataactgtgc aacgatcttg
    11881 aatacttcgt catcgggtaa gtgcttctta ccgaaatcat gaaatgcagt aaatcttgga
    11941 tcagtaattc taagaactgc atgaccatac ccgataacga gtccgcctct gtcaagcaga
    12001 tcctgacaat attttctgac ttcatcatct gtaggtgagt gtccgatatc ttctttcaat
    12061 ttcaaaatga atttaagaca gttctgattt gcaagaccgt gaagtggacc ggcaagtcca
    12121 ttcaatgctc ctgtaatgga ataatatgca tcagaaagtg cagatgaaat taccagacaa
    12181 gtgaatgcgc tgacatttcc gccttcgtga tcactatgca atacaagata aagtctgagt
    12241 aattttgcaa atgtaccttc tttgtcatct actccaagca tctttgcaaa attttctgcc
    12301 cagtctaaat ttttatccgg cggtatcata tcgccttttt taaatttctt tctgtaaata
    12361 aatgctgcga tctgcggaac tctggctatt atatttaaac aatcttcata catcggttcc
    12421 caaagtttat ctttacccaa tccgttatta tattcatttc tgaattcaga ttctttttcc
    12481 attacattaa ttgcagtact gaacataacc atagggtgtg aatcctcagg cattgaattt
    12541 aatacatccc atacatatga aggaacgtca cttctctcat gaaggtcatc ctgaagatct
    12601 ttcagttcag tttcatttgg aagctttccg attaccagga gataaaaaat ttcttcaggt
    12661 gatctatcag ttaattccag gattggaatt ccccgtatta caagtcctgt ctgcagacca
    12721 acttcagaag tgtcgcagat cagacccttt atacctctca ttccgccata aagctgttca
    12781 attgcaacct gacctattac ttcagagcca tgattttttg caattgattt tgcctcttct
    12841 cttaaagcgg gtaactgaga agctaatctt tctttcatta attttgacat gtgtgataaa
    12901 ttttaatatt cgaattccaa tataattctt ttgattttaa gtataaagat aagaatagag
    12961 tactctatag gttttttaaa tttttataaa aaagacttaa actcttcaaa atttaatgat
    13021 ttcatttgtg tgaaatttta atatattttc aaatccatag ttaattaaaa agttactttt
    13081 aatatcttta aatttgggaa tataaaattg ttcagtgttt tcgcactatt accaaagtca
    13141 ttttatttca aattgaatta gtttttatta aatggtaatt taatccatat ttaaaaatta
    13201 acgaaattat gtctgaaatt atttatcatg gtcattcctt tttagaaatt aaaactactg
    13261 atcttaccat atacattgat cctttcatca ccgggaatac tttatgcgat ataaaaactg
    13321 aagatgcaaa atgtgattat atttttttga cacatggtca taatgatcat gtaggagaca
    13381 ctattgaaat tgccagaggt aaaaacatta ccgtaatagg tgttgtcgaa ctcactgact
    13441 atctcgaaag taaaggaatc agtgtgcacg gtatgaatat aggaggagca tacacttttc
    13501 catttgggaa agttaaacta acaattgctc atcattcttc ttcaattatg aatggtccgt
    13561 atgcaggaga acctgccggt ctaatcctga acatagaagg taaaacgatt tatcactcag
    13621 gtgatacagc tttattttac gatatgaagc ttattggtga aatgaataat attgattatg
    13681 cttttcttcc gattggtgat aattttacta tgggaattga tgacgcagta aaagctgccg
    13741 gttttttaaa tgcaaaaaat attataccaa ttcattataa tacttttgat ttaataaaag
    13801 ctaatgaaaa agaatttaag agaaagatag aatcaatagg aaagatttgt actgtcatga
    13861 acccaggtga ttctgtgaat ttttaatatg ccttttgatt atgggaaaat aatctgaatg
    13921 aaaacaatta attatatttg acaaaaatta tatcaatatc aaatcagaaa ggcggagtcg
    13981 gtaaaactac tactgcaatt aatctatctt ctgcaattgc tgcttacggt aaaaaagtac
    14041 tgatgataga tactgaccca caggcaaatg caacatccgg gcttggtctt gataattcta
    14101 ttgaaggaaa ttcaacatat gaaattttta tatctgataa aaaagtaaaa gattgtatta
    14161 aagatactca ggttcaaaat ctgaaaataa tctctgcaaa tataaatctg gtggcagcgg
    14221 agcttgaact agtagaccta cctcacagag aaagtattct gaaaaataag ttaaaagaat
    14281 ttcttgcaga aaacatcgaa gattttgatt ttatattagt tgattgcccg ccatcgcttg
    14341 gattaataac tgtcaatgca ttgactgcag cagattctct actcattcca gtacagtgtg
    14401 aatattatgc tctcgaagga ttgtcacagc ttctcaatac aattaaaaat gtaaaaaata
    14461 tttttaatcc ggatttaaat atcgaaggct atcttcttac aatgtttgat tcccggctta
    14521 agctttccaa tcagatagaa tctgagctta ggaaattttt tggtgaaaaa gttttcacta
    14581 caaaaatatt cagaaacgta aaaataggag aggcaccaag ttacggaaag ccaataattg
    14641 tttacgatcc ggcttctacc ggggcattga actatatgga tctcggcaaa gaatttctct
    14701 taagaaacgg ttttgaaaca gagtctgaga agaattctga taattcaaat aattccgaaa
    14761 taaatgaaat taaagtaaat gaaacataaa tttttttaat atggaaaata aaacatcagc
    14821 attaggaaag ggattgtcgg cgatttttga tgaaaagaaa attgatgttt cctttgtgga
    14881 agaaagtgca aaaaagaatt caactgtttt aaacgtatct gagattcggg taaatcctgc
    14941 ccagccgcgt gaagattttg atgaggaaaa gctaaaggaa ctttcagatt cgatcagaca
    15001 gaaagggctc attcagcctg taactgttaa gaaaacggaa aacggatatg tccttgtatc
    15061 cggtgaaaga agacttcgag ctgtaaaatt gattggaatg gaaaagatcc ctgtctattt
    15121 catggaaaat aataatgggt ctgatgaaga tatgcttgag cttgctctga tagaaaattt
    15181 gcaaagagaa gatctgaatc ctatggaatt atctgattca tattttaaac taatcgaatt
    15241 acataatctt actcaggaaa aaattgcaga aaaagtttca aagaacagaa gtacagttgc
    15301 taattatctc agactgcaga gacttccaat tgagattaaa atttccctga gaaaaaacga
    15361 gatcacagaa gctcatgcaa gacttttgct cagagtagat aatgaagatg atcagatcac
    15421 attatggaag agaattgtga atgaaaatat tacagttaag aatttacagg aaattacaaa
    15481 acaggatgtt aaaaaaccaa aagctaagaa gcctaaactc ggtgaaattt atgatccgta
    15541 ttttcaaagt gtagaggata aactcagaaa ttttttaggt acaaaagtat cagtaaaaag
    15601 aaaatcaaaa tattcgggtg aaattattat tgaatatttt tcaaatgatg atcttgaaag
    15661 gatcatcgaa aagtgttcag actaatattt tttaaaaaga atttatataa accaatttta
    15721 tgaaaagcat tttaatctcc gataaagcag cacctccggt tggaccatat tctcaggcag
    15781 tcggcatagg tgatctgatc tatacctcag gtcagattcc gtttgatgtg gatggaaatt
    15841 tagtgtcgga taatattgaa ggtcaaactg aaaaggtact tgaaaatcta aagtcaatat
    15901 tagaagacaa cgggtcttct cttgataatg ctgtaaagac aatggtgttt ttaaatgata
    15961 tgaataattt tgcaaagatg aatgaggttt attcgaaata ctttgtaaat tctccaccgg
    16021 ccagaagtac agttgaggtg tcaagacttc ctaaagatgt attagttgaa atagaaataa
    16081 ttgcatttaa aaacaaataa attttaacag tgaatccatt acatgacaga ttgtgtccgg
    16141 gtaaattaaa gtttactgtc ggcattgtaa taaaatccga tttaagaact gtctggaata
    16201 atgtatgcaa agctgaacag gtcaggaaat attttactac tgatgcaaga agagatctgg
    16261 acagcagcgg agatgtactt tggatctggg gaaatgaagc agctttaata aaagtggtcg
    16321 aaatactccc cgaggataag cttgtgtttg aatggaacgg ttcaaatgta gattacagga
    16381 ttcgtacgga atttatcttt aaaaaagaaa atgataaagt aactttaaaa atcaaagaaa
    16441 agggttggga agaaaatgca aaagggatga agagtgcatt tttaaactgt agtggctgga
    16501 cagaatttct tagtgcatta aaagttttca cagaatatca aatttcctat ctgaaataaa
    16561 aaaagtccgc attcagcgga ctttaataat acttatttta ccgaaatcta tttttcgagt
    16621 ttgtttatat gcttagcaat ctttgacttt tgatttgcag ctttgttttt atgtatgatt
    16681 ccttttactg cagctctgtc aaggacctgt gaggtctttt taaattctgc tgctgcttct
    16741 gatttctctt cttttgacat gactcttttc actgaacctt tgattaaagc tttatacttt
    16801 ttgtttcttt cggttttttt gacagtttgt cttgctcttt tttgtgctga tttatgtctt
    16861 aatggcattt gtaaaattta aattttatca aaatacttat ttcaaagcga aaaatcaaag
    16921 cataagaaaa gtactgagta aattttttgt atcactaaca tatttataat aaaatattca
    16981 ctccctttta aaataattaa tactcctgta tattgtaaaa aacaaaggat caagataaat
    17041 tataaataat gccattctta cactataaaa tgatacaaaa atcgaaataa caaaaattaa
    17101 tacgggtata taagacaaga tcttagtttt agtataatct tcttcactta tgctatctgt
    17161 aaagacatta ctcttagaaa agatataatt taggtgtatg atcataatta tactgatcag
    17221 aattagattt aaagcataaa taagaaatgt aaattctgat ctggggtatt tcattaatac
    17281 accaactgta aaaggaataa aacctgtcag catcagaaat aggatattta aaactataat
    17341 acttcggtct acagtagtaa taaatttaaa ctgtatctga tgtctgagcc aaaaaactcc
    17401 aagtacaaga aaactaagca catatgcttc aatagccgga agtaaattga aaaacatatc
    17461 tggaagttcg aaatgaatca gatctttcgg gatattttca ggggttttta aatcaagaat
    17521 gatgattgtc attacgattg caaaaatacc gtcacaaagt gcttctactc tgtgttttgg
    17581 tagaagactt tcatcattaa tatccagatt attttttcta aaaagtccca cttaggaaaa
    17641 tatattgtat atatataaaa atctcaaatg attaaattga gcattgtaaa ataaataata
    17701 acatgggaaa taaaataact ttaaatactt taaattctaa ttcaaataat aattctaata
    17761 taaataaaaa tattcttaat gttccggatg atccgatcat tccatatata gatggtgatg
    17821 gaattggacc tgaaatctgg agaactgcaa gacatgtttt tgacagtgct gtagaaaaag
    17881 cttataatgg taaaaggaaa gtagagtgga tggaagttct ggcaggtggg aaatcatttg
    17941 aaaaaacaaa aagctggctt cctgatgaaa cacttgatgc ttttcgggaa tatctagttg
    18001 gaataaaagg tccattgaca actccaattg gaaagggaat ccgatccatc aatgtagctt
    18061 tacgtcagaa actcgatcta tatgtttgtc taagacctgt taaa
//
LOCUS       DAORVQ010000046        18032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1288_length_18032_cov_5.018969, whole genome shotgun sequence.
ACCESSION   DAORVQ010000046 DAORVQ010000000
VERSION     DAORVQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 18032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18032
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_18032_cov_5.018969"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..611
                     /locus_tag="PLX80_05630"
     CDS             240..611
                     /locus_tag="PLX80_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20310.1"
                     /translation="MISKVQKIFIFTLLVIIAGCSSSQSSMYKPGDGGDGWKVDVTKK
                     DGLTEEFICKINGTTVMSDSFPLIGDNFEKTGTYKGKKVVMNGFRNTAATVGADGKVQ
                     NKDTFQIRVFIDDQLVDKFDF"
     gene            776..1891
                     /locus_tag="PLX80_05635"
     CDS             776..1891
                     /locus_tag="PLX80_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY20311.1"
                     /translation="MVKIFAEDLKAIREEKNISLKSISQQTRINLSALENLENGDYSF
                     QPQAYIRAFLKQYIACLDLDVEEVLFDYDLARSGKYKSNRPEKEIAEEKIIEKVSDKS
                     DDKIVLEIDNETDSSKEEKISSGDEFSEVDLNVENTDEDEKTFKRAETPSTPKLKRIR
                     TEPESNIGTSGIRKEKPTLKSVEDRSVSQYSRMPQKKSFSFAFLKSRIFRNILMTLFI
                     LLILAGIYSLINILFLEGKSDNPEIIRQNFDEVIQEQEKKLLGKKTEEEIQDSIKKAN
                     EESSAPGDSITLKVTALSESVFYIVTDSSNYSKPEKISLDPKDVKVFKAAGSFHISTS
                     NTNSLNISVDENPLEFEKTAVSKVKITKDGFVKPSSK"
     gene            1916..3931
                     /gene="ligA"
                     /locus_tag="PLX80_05640"
     CDS             1916..3931
                     /gene="ligA"
                     /locus_tag="PLX80_05640"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQY20312.1"
                     /translation="MSPSPEIIKKINSLREKIEEADHKYYTLADPDIDDFAYDKMYKE
                     LSELETEYPDLISSDSPTQRVSGKPAANFKTVVHRIPMLSLSNSYNFEELLEFDKRIQ
                     NLLETKNYEYICELKFDGIAISLIYENGFLTTAATRGDGIKGDDATQNIKTIRSIPLS
                     VRNDSIKNFEVRGEVFITKDDFIKINEEQEFKNEKLFANARNTAAGTLKLKNTKIVAT
                     RPLNFYSYYFYGENVKVDSHSAGLDILRDLKFPVNNKTKTAKNIAEVKEFCDNIELIR
                     DELDYEIDGVVVKVNKLSQQEILGSVSRSPRWAVAYKFKAKEAVTKINNIYCQVGRIG
                     TITPVADLQPVFLAGSTISRATLHNFDEIKRKDIREGDYVKIEKGGDVIPKVLEVIID
                     KRKKDSKPYEIPENCPVCKTKLEKPEDEVYYYCPNYTCEAQILGRIEHFVHRNAMEIE
                     GLGTSIIEIFNQNGLLNNYADIYSLYKHKKNILELERFGEKSVENILNAVEQSKDKPF
                     DKVLFGLGIRHIGERTSKILAKNFQNIDNLIKATGEEITHVHEVGPKIAESVIKFFKD
                     KKNLKNIENLRKAGLKFETDKKPGNEVTNENFNNKTFVLTGTLEKYKRDEAQKIIEDL
                     GGRVSSSVSKKTDYLLAGSEAGSKLEKARSLGVKVLEEKEFEKLIKQ"
     gene            3950..4453
                     /locus_tag="PLX80_05645"
     CDS             3950..4453
                     /locus_tag="PLX80_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQY20313.1"
                     /translation="MAFTIDTSAASDKSEIYKTLIPQIKSLIEGENNLTANLGNICAA
                     LKYSFYSFIWVGFYLKSINNKNELILGPFQGRVACTRITFGKGVCGKAAENKETVIVD
                     NVNEFPGHIVCDPLSKSEIVIPVIINKETVAVLDIDSGEYSNFDETDKEYLEKMIDNI
                     KHIFKNE"
     gene            4446..4691
                     /locus_tag="PLX80_05650"
     CDS             4446..4691
                     /locus_tag="PLX80_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY20314.1"
                     /translation="MNRKELIKIVSEETGLKKKKCERAVESAFELISQALIKGSKVSI
                     TNFGSFRTQKIGSEIYCDGSFVNIIPPSVMINFEKNI"
     gene            4709..5191
                     /locus_tag="PLX80_05655"
     CDS             4709..5191
                     /locus_tag="PLX80_05655"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY20315.1"
                     /translation="MNRTELISGVSIQCGISRQEAESILNSIFKNISDSLKEGEDVKI
                     KSFGEFKPRASLNGKEKTDRTEFKPAKKFYSKINKLYEYLTPSRKIYDKTRLENIYNE
                     FGIKDFIPADIHSEKINENKLTENNELPEKRLISDKLINLHNEIVNPEKKSVKKNLWG
                     "
     gene            complement(5192..6865)
                     /gene="lnt"
                     /locus_tag="PLX80_05660"
     CDS             complement(5192..6865)
                     /gene="lnt"
                     /locus_tag="PLX80_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQY20316.1"
                     /translation="MLVRNKDIPDSGWLFKILIPLAGVLFGLSFPPVDLSFLIFFSFA
                     IQIFINLRSKSFKSVFLRSYLIYFIASLFAVSWVMFSGMRENADRFLIIGGFFILLTY
                     PLFFVMPMLVYYKIKSSFTFRSGFLISLLLFPVIWTGFEYISTLGQINFPWLFAGNTM
                     TYQPSKIQYAEITGIFGVSFWVCCISVLLYLLFHNIYEKKFRPFTAGNISTALILLLI
                     YFFPDFYNSNLSDYKKFHSQSDEKIKIGIIQPNVDPWKKWGGKQSDLITGYVSQIKEL
                     NDSSSDIDLFILPETALPYYYRERYFEDNLQIIKTFCDSINIPILIGTPDLEYYEDQE
                     AAPVDAKIMRSTGNKYDTYNTAVLFEPDREVNDFQKHRKIKLVIGSERMAYQEYLPFT
                     KSLIEWGVGLGSWQIGNDSNIFVLNNLNSESKFNTAVCYESVYPEFFAEFVNKGADFC
                     VIITNDGWWGKFFGTYQHNQFAVFRAIENRRWIARCANTGISDFISPYGELFQETAIN
                     EKAKIVREISLIKEKTFYTQHGDFLARYFLYISLFVFALSFFIKKKKNE"
     gene            complement(6871..7443)
                     /locus_tag="PLX80_05665"
     CDS             complement(6871..7443)
                     /locus_tag="PLX80_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQY20317.1"
                     /translation="MLEILQNIDAALFYFINNILANPFTDRLMPFITERDHWFIFYVL
                     IWLYLFFKGGRKGKVSAILILLLILLADQFSNNILKSYFQRTRPCHVLTDVNLLINCS
                     ESFSFPSNHAVNNFAAAYLFSNFYPRMKGFLFTGAALVALSRVMCGVHYPFDIMAGAV
                     IGILFAMLIIYLWNLFNKKFGIIKDPEALT"
     gene            complement(7450..7920)
                     /gene="ispF"
                     /locus_tag="PLX80_05670"
     CDS             complement(7450..7920)
                     /gene="ispF"
                     /locus_tag="PLX80_05670"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQY20318.1"
                     /translation="MNFRTGFGYDVHRFSNERKLILGGVEIPFDKGLAGHSDADVLLH
                     AVCDSLLGAAGFKDIGHQFPDTDPEYKNISSIVLLKRSFELIKSKGWEIANIDCTLVL
                     EKPKIYKYTDQMKKNISDIILTNEISIKATTSEGIGFVGREEGCVAYATVLLYK"
     gene            8119..8204
                     /locus_tag="PLX80_05675"
     tRNA            8119..8204
                     /locus_tag="PLX80_05675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8153..8155,aa:Leu,seq:taa)
     gene            complement(8375..11233)
                     /locus_tag="PLX80_05680"
     CDS             complement(8375..11233)
                     /locus_tag="PLX80_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY20319.1"
                     /translation="MDKIPSGTVTFLFTDIQGSTKLAQENHDKYIASLEKHHEILYEV
                     IDSKDGFVFKIVGDSFCCAFESPVNAVRAAIKTQIKLKENEWDGSEIKVRMGIHIGEA
                     EFIKDDYAGYVTLSRSQRIMSIAYGGQIIVSQIVFDTLKDLNLKDFSFKDFGIRKLKD
                     IIVPEHLYQIVAEGLEDDFPPLKTEDARRNNLPVSVSKFIGRKKEIEEIKELFANIRM
                     LSLIGSGGTGKTRLAVEILGGVLDEFENGVWLIEFSPVTDPELIVSEITTVLNVKEEP
                     GKKLLETLKEYLKGKHIILLFDNCEHLLDKCSSIAMELLSSCPKLKIISTSRMSFNIP
                     GETIYRIPPLSIPENIKNKTFKSLSEYESVKFFLEIANSINSNFNLTEDNIYYVAELC
                     KRLDGIPLAIELAAKRINVLTVDKILDRLDNRFKLLTGGGSTALPRQKTLKAMIDWSY
                     DLLEADEQELLQRLSIFMGGWTLEAAEEICSDDKIDMYEVLDLMTSLYDKSLITFKEV
                     KGIGRYGILESIKYYALEKLDGRKEEYQKHLNYFIDFSSLEKHKAMGQFEWLNVMETE
                     IDNIRRNIQWAIENNSVDAVKLVLNVYDFWLNKGYLQEGYETLMKIHNTINLNDKKLK
                     ADLINRISRLCYELGKFDDLLKFSNEALELYREINDKEGILKSLNILALKSYTELDNA
                     SAVQYNEEALALSNEISSMESKADSLYNLSFPVGNLGDFERSILLKEEALEIAREINS
                     DHLKAQALLSLSVTHSRRTGDIKKASVCSDESLIISRRIDDRYLISVNLVHLAALKLY
                     YENKNFEEAEYLLLEAYDMSIEWGYKMNLFPIRIHLGFLYIETERYQQAIDIYSEYIS
                     EREKPGGEFFMLDLLAGFGRLYFKKNEFERSFKLFGFIEKLSKDDKYKAIIKALALND
                     EDREIILKNLGEEKFNLCRKEGMEMTIDEALELCLKIE"
     gene            11502..12683
                     /locus_tag="PLX80_05685"
     CDS             11502..12683
                     /locus_tag="PLX80_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20320.1"
                     /translation="MKYFLKFALLVILFLSFNNITISEVISENGKKSSSQDSRLVNPL
                     YELRAKDLISSSNPLIDPNIYYFDLVISHTNLLESGPFEYAAGQYTLSFNWLIRLFGP
                     LTYEIVPNTSDFTNPDALPVNPRVVGNKLILDRNPNLIAGQGPIISPNIEGTRIVRMK
                     VTTSVPDFLNIPLKLKWISNPTDSNTTAVYAFIEDSVTNISSGGTLLIDSGSVAALNL
                     KAAISGLYNQTSNQLRARDTLRVFQNSITSPYNIVDSTRTIIDSISFTGVCNFMNATS
                     GKYYTVIKYKNGLETWSRSGGDSLKRGSQVSYDFTDDSTKAFGNNLEKKGILYCIYSG
                     DVNQNGIIDIADLTLVDNGIFNFISGYSILDINGDNFVDLTDSQLTDNNAYNFVTVIK
                     P"
     gene            complement(12784..14361)
                     /locus_tag="PLX80_05690"
     CDS             complement(12784..14361)
                     /locus_tag="PLX80_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HQY20321.1"
                     /translation="MNIDFNLNRKLFENSDFGPKPGTVEEQILFEKIQEGLAYQYENI
                     FPDKYAPRTVVIIPSLSLDMEILSKIKGSIHYEERLLCLLMLLRMPMTKIIYVSSMPL
                     SEVIIDYYLHLLQGITGSHARKRLTMFSCYDLSRKPLTEKILERPRLIERIKSLLTDK
                     ASTHLTCYNITPLEKSLAVKLEIPLFGTDPSMFYEGSKSGARKTFRLCGIEMPDGDED
                     LKSKEEIVRSLARLKRKDPGLRKAVIKMNDGFSGDGNAIYRYPDLEIDEFLETKIFDT
                     LYQYTRPVSHDVSLDLFFEKFIEMEGIVEEFLEGEIKVSPSVQCVIGASDKIRIDSTH
                     DQLLGGDDGQIFLGAIFPADKEYASELGVIGKKVADLLSKKGVLGRFAVDFISVKQDD
                     GKWIHYAIEINLRKGGTTHPFMMIEYLTYGKYNSEEGVYITASGNPRYYFASDNVQSD
                     NYIGITPSELIDIAMFHGLMYDGATQEGVVFHLIGALSEFGKLGVVCIGSSPERANEF
                     YDKTISILDLECSDKNE"
     gene            complement(14450..15712)
                     /locus_tag="PLX80_05695"
     CDS             complement(14450..15712)
                     /locus_tag="PLX80_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQY20322.1"
                     /translation="MRLVNNKDLKKRSMILISKLKKILLFFFISVNILYAQNEFEIVE
                     STPVETVYENSTLAKTPDVWKKMIDDASVSVDIETFYFADETNEPLEIIMGSLKNAAE
                     RGVRIRIIVDSSFYSRNDKSVDKLEGINNITIKKIPFGNLGGGVMHAKFFIVDNENIF
                     LGSQNFDWRALKHIHEIGLRVKDKNLAQTFTELFETDWNLCDGNISVLVNQRPVAIVN
                     SEKPVTIKSEKYGEIILYPAFSPPDYNISGVSNEEDELINIISAAKENLLIQIYSFSD
                     KDRYDTVQFDRIANSLKEAAGRNVQIKMILPDWEIGGSSQDFIKEFSQIKNIEVKIIS
                     IPLYSEGFIPFARVDHSKYMISDNDVSWISTSNWESGYFRHSRNVSIIIKNKLVNNDL
                     RNVFEMGWRSPYANEIEINKNYEPVKRN"
     gene            complement(15714..16604)
                     /locus_tag="PLX80_05700"
     CDS             complement(15714..16604)
                     /locus_tag="PLX80_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQY20323.1"
                     /translation="MTTDSDVTITPNKEFDHLIKLMDDEDVNIYKSVKDRFLSYGILS
                     EDFLKGYLNDENLLIKKRANEIISILNFTNLEYQFNKLKHSQSKQYLEKSIFLIASYG
                     YPLVDMKKYEKITDEYVSEIKQRVINKFRKVEPDYPLDLLETINEYLFGELKFKGNKE
                     NFYDPDNSFINKVIDNKTGIPITLSVIYLIVAKKLNLPIYGINMPGHFLLKYENKNAE
                     YYIDPFNEGIIISGSEAERFAKNIGIQKSDFDQIPYLNKSTDNEILLRILRNLLEIYR
                     ENKEELKVSQIEKLMNILVI"
     gene            complement(16601..17812)
                     /locus_tag="PLX80_05705"
     CDS             complement(16601..17812)
                     /locus_tag="PLX80_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY20324.1"
                     /translation="MKNKSQVLVWSLFDFGNSSYAVIIVAFVFAVFFKEVVVSGESIG
                     DLYWSIGINISMIISAVLNPVFGAIADYSSNKKKFLFAFTILCVISTSLMYFTGEGTI
                     FFALVLFILSNIGFQTGLTFYDAFISEISEPKDYNKVSGYGYATGYVGSVISVLLVFP
                     FKDDPNILFVICAGFFLIFSMPLFLFLKEEKKSNGPVKINYIAYGFNKVFNTLKNLNN
                     FKDLKNFLISFFFYIDAVNTIIFFSGIYASSTLGFSITELAIFFLIVQITAMIGSYLF
                     VLIGDKMGIIKSLNINLIFWTLLTIAVFVTNDKNSFFIIGGFAGLFLGSTQSLSRALM
                     TYLTPLDMKTEFFGFYALMDKTSTLIGPLTFGLVSWISGSQKLAILSVGLFIIIGMFL
                     LKKVKVKEILK"
BASE COUNT         6256 a         3064 c         2830 g         5882 t
ORIGIN      
        1 ttgattcaag ttgaaatcat ataagtcata taaatagatt atgctggagt ttgttgatga
       61 tggttaaaaa gtttttgtga aaaaaatcgc agaattcaaa ttaggttata aaacttttta
      121 gaactccaac agcagcttaa cgctcagcca ttattttatc tctaaatttg gaatcatttg
      181 ttaataaatt aatagttaaa ttttcaaaat tttttataat ttattaaact attaatgaca
      241 tgatttcaaa agtacaaaag attttcatat ttactttact tgtaattatt gccggctgct
      301 catcttctca gtcctcaatg tataaacccg gtgacggagg agacggatgg aaagttgatg
      361 ttacaaaaaa agacggttta acagaagaat tcatatgcaa aataaacggt actacagtaa
      421 tgtcagactc ctttcctttg ataggtgata attttgaaaa gaccggtaca tacaaaggta
      481 agaaggttgt tatgaatggt tttagaaata ctgctgctac agttggcgca gacggtaaag
      541 ttcagaataa agatactttt cagataagag tatttataga cgatcagctt gtagataaat
      601 ttgattttta aaattcaggt tttaataagt agatttttct actaagaaat atctctctct
      661 attatttata atttactttg aaaattgttc attgcacatt atacattgaa cattgtgttt
      721 attatgtact ttaaattttt cttaatttca gataacttgc ttaaaaaact tattgatggt
      781 aaaaatattt gcagaagatt taaaagcaat tcgagaagag aaaaatattt cattaaaatc
      841 catatctcaa caaacacgca taaacctttc agctcttgaa aatcttgaaa atggtgatta
      901 cagttttcag cctcaggctt atataagagc ttttttaaaa caatacatag catgtcttga
      961 tcttgatgtt gaagaagttt tatttgatta tgaccttgcg agaagcggta agtataaatc
     1021 caatcgtccg gaaaaagaaa ttgcagaaga aaagattatt gaaaaagtat cagacaaatc
     1081 tgatgataaa atagtattag agattgacaa tgaaacagac agttcaaagg aagaaaaaat
     1141 ttcatccgga gatgaatttt ctgaagttga tcttaatgtt gaaaatactg acgaagatga
     1201 aaaaactttt aaaagagcag aaactccttc aacaccgaaa ttaaaaagga taaggacaga
     1261 acctgaatca aacataggta cctcaggcat aagaaaggaa aagccaactt taaaatcagt
     1321 agaagacagg tcagtttctc agtattcaag aatgcctcaa aaaaaatcgt tttcattcgc
     1381 atttctgaaa tcaagaatat tccggaacat attgatgact ctgtttattt tgctgatact
     1441 tgcaggtatt tattctctta ttaatatttt attccttgaa ggaaaaagtg acaaccctga
     1501 gatcataaga caaaattttg atgaagtaat tcaggaacag gaaaagaaac ttcttgggaa
     1561 aaaaactgaa gaagaaattc aggattcgat aaaaaaagca aatgaagaat catcagctcc
     1621 aggagattcc atcacattaa aagtaaccgc attaagtgag agcgttttct atattgtaac
     1681 agattccagt aattattcaa aacccgaaaa aatttctctt gatccaaaag atgtaaaagt
     1741 ttttaaagct gcaggatcat ttcatatttc gacttctaat acaaacagtt taaatatatc
     1801 agtggatgag aatcctctag agtttgaaaa aactgcagta agcaaagtca agataacaaa
     1861 agacggattt gtaaaacctt catctaaata aaaagaatta acgaaaatta tttatatgtc
     1921 tccatcacct gaaattataa aaaaaattaa ttctctcagg gaaaaaatag aggaagcaga
     1981 tcataaatat tatactcttg cagatccgga tattgatgat tttgcatatg ataaaatgta
     2041 caaggagttg tctgaactgg aaacagaata tcccgacctt atttcttctg actcacctac
     2101 tcaaagagtt tccggaaaac ccgctgcaaa ttttaaaact gtagtacata gaatcccaat
     2161 gctctctctt tcaaattcat ataattttga agagctcttg gaatttgaca aaagaattca
     2221 gaacttatta gaaactaaaa attatgaata tatttgtgaa ctaaaatttg acggaattgc
     2281 tatttcacta atttacgaaa atggcttttt aacaactgca gcaacacgcg gtgacggaat
     2341 caaaggagat gatgcaactc agaatattaa gactatcagg tcaattccac tttctgtcag
     2401 aaacgattca ataaaaaatt ttgaagttcg cggtgaagtt tttattacca aagatgattt
     2461 tataaaaata aatgaagaac aggaattcaa aaatgagaaa ttatttgcta acgcccgtaa
     2521 tacagcagcc ggcacattaa agttaaaaaa tacaaaaata gttgctacaa gacctttgaa
     2581 tttttattca tattatttct atggtgaaaa tgtaaaagtg gattctcatt cagcaggtct
     2641 tgatatttta agagatctta aatttcctgt aaataataaa accaaaactg caaagaatat
     2701 tgcagaggta aaagaattct gtgacaatat agagctgatc cgggatgaac tggattacga
     2761 aattgacggt gtagtggtaa aagtaaataa actttcacag caggagatac tcggcagtgt
     2821 ttcccgttct ccgaggtggg cagtcgcata taaatttaaa gccaaagaag ctgtcacaaa
     2881 aattaataat atctattgcc aggtcggaag gataggaaca atcacgcctg ttgcagattt
     2941 gcagcctgtt ttccttgcag gttctactat ttcaagagcg acacttcata attttgatga
     3001 gataaaaaga aaagacatcc gcgaaggtga ttatgtaaaa attgaaaaag gcggcgacgt
     3061 tattccaaaa gttcttgaag ttatcattga taaaagaaaa aaagattcaa agccgtatga
     3121 gatacctgaa aattgcccgg tctgtaaaac caaattagag aagccggagg atgaagttta
     3181 ttactactgt ccgaattata cctgcgaagc acagatacta ggcaggatag aacatttcgt
     3241 tcacagaaat gctatggaaa tagaaggtct cggaactagc attatagaaa tatttaatca
     3301 aaacggatta cttaataatt atgctgatat ttattctctg tataagcata aaaagaatat
     3361 acttgaactt gaaagatttg gagaaaagag cgttgaaaat attctgaatg ctgttgagca
     3421 gtcaaaagat aaaccatttg ataaagttct tttcggatta ggcatcaggc acattggtga
     3481 aaggacttca aagatcttag caaagaattt tcaaaatatt gataatctta tcaaagctac
     3541 aggggaagag atcacacatg ttcatgaggt gggtccaaag attgcagaga gcgtgataaa
     3601 attttttaag gataaaaaaa atcttaagaa cattgagaat ctgcgaaaag ccggattaaa
     3661 atttgagact gataaaaaac cgggtaatga agtgaccaat gaaaatttta acaataaaac
     3721 ttttgtgctg acaggtacac ttgaaaaata taaacgcgat gaagctcaga agataataga
     3781 agatctcgga ggcagagtga gttcatcagt aagtaaaaaa actgattact tgctggctgg
     3841 ttctgaagcc ggcagcaaac tagagaaagc aagatcgctt ggagtaaaag tcttagaaga
     3901 aaaagaattt gagaaactga tcaaacaata ataattttta aaaagtaata tggcatttac
     3961 aattgataca tccgcagcat ctgacaaatc agaaatttat aagacattaa ttcctcagat
     4021 aaaatctctt atagaaggtg aaaataatct cacagcaaat cttgggaata tctgtgcggc
     4081 tttaaaatat tcattctata gttttatctg ggtcggattt tacctaaaaa gtatcaacaa
     4141 taaaaatgaa cttattcttg gtcctttcca gggaagggtt gcctgtacac gaatcacttt
     4201 tggaaaagga gtatgcggga aagcagcaga gaataaagaa actgtcatag tagataatgt
     4261 aaatgaattc cccggtcaca tagtctgtga cccactttct aaatccgaga ttgtaatacc
     4321 tgtaataata aataaagaga cagtcgctgt tcttgatatt gacagcggtg aatattcaaa
     4381 ctttgatgaa actgataaag aatatcttga aaaaatgatc gacaacatta aacatatttt
     4441 taaaaatgaa tagaaaagaa cttattaaaa ttgtcagtga agagacagga cttaaaaaga
     4501 aaaaatgtga acgggcagtc gagtcagcat ttgagcttat atcacaggca ctgataaaag
     4561 ggagtaaggt ttcaattaca aatttcggca gcttcagaac tcaaaaaatc ggatctgaaa
     4621 tttattgtga cggatctttt gtaaatataa ttccgccttc agttatgatc aactttgaaa
     4681 aaaatatctg agacttatga acagtgaaat gaacagaact gaattgatct caggtgtatc
     4741 gatccaatgc ggaataagca ggcaggaagc agaatctatt ttgaattcaa tttttaaaaa
     4801 tatatcggat tctttaaaag aaggagaaga tgtgaaaata aaatcatttg gtgaattcaa
     4861 accgagagca tctttaaatg gaaaagaaaa gacagacaga acagaattca aacctgcaaa
     4921 aaaattttat tcaaaaataa ataagttata cgaatacctt acaccttcca gaaagatcta
     4981 tgacaagaca agattagaaa atatttacaa tgaatttgga ataaaagatt ttataccggc
     5041 tgatattcat tcagaaaaaa ttaatgaaaa caaattgact gagaataatg aattacccga
     5101 aaagagactt atctcagata agctgataaa tctgcataac gaaattgtga accccgaaaa
     5161 aaaatccgtc aaaaaaaatc tatggggatg atcattcatt ttttttcttt tttataaaaa
     5221 agcttagtgc aaaaacaaat aatgaaatat aaagaaaata ccttgcaaga aaatctccgt
     5281 gctgagtata aaatgttttt tctttaatta aacttatctc tcttacgatc ttagcttttt
     5341 catttatagc agtctcctga aacagttcac cataaggact tatgaaatca gatatgcctg
     5401 tattggcaca tcttgcgatc catcttctgt tttcaattgc tctgaacact gcaaactgat
     5461 tatgctgata tgttccaaaa aattttcccc accagccgtc atttgtgatg attacacaaa
     5521 agtctgcacc tttgttcaca aattcagcaa agaattcagg atatacagac tcgtagcaga
     5581 cagcagtatt gaatttggat tcagagttaa gattattcag aacaaaaata ttcgaatcat
     5641 ttcctatctg ccagcttccc agtccgactc cccattcgat cagacttttt gtaaatggaa
     5701 gatattcctg atatgccatt ctttcacttc ctattaccag cttgatcttc ctgtgtttct
     5761 gaaaatcatt tacttccctg tccggctcga ataaaactgc ggtattatat gtatcatatt
     5821 tattacccgt actacgcatg atctttgcat ctacaggcgc ggcttcctga tcttcgtaat
     5881 attcaaggtc cggtgttcca atcaggatcg gaatatttat actgtcacaa aatgtcttta
     5941 tgatctgcag attatcttca aaatatcttt cacgataata ataaggaagt gctgtttcag
     6001 gtagtataaa cagatctata tcagatgaag aatcattcaa ttcctttatc tgcgagacat
     6061 atccggttat gagatcgctt tgtttacctc cccacttttt ccagggatct acattcggct
     6121 ggatgatacc tatcttaatt ttttcatccg attgcgaatg aaatttttta taatccgata
     6181 agtttgaatt ataaaaatcc ggaaagaaat aaataagtaa tagaattaat gcggttgaaa
     6241 tattaccggc tgtgaaagga cggaactttt tttcgtaaat gttatggaat aaaagatata
     6301 gaagtacaga tatacaacag acccagaagg agacgccaaa tatacctgtg atctcggcat
     6361 attgaatttt cgacggctga taggtcattg tatttccggc aaaaagccac gggaaattta
     6421 tctgaccgag agttgagata tattcaaagc cggtccatat aactggaaaa agtaaaagag
     6481 aaattaaaaa tccggatctg aaagtaaagc tactttttat tttataatat accagcatcg
     6541 gcattacaaa aaacaaagga tacgtcaata aaataaaaaa tccaccgatt atcaaaaacc
     6601 tgtcagcatt ctccctcata ccgctaaaca ttacccagga aactgcgaac agagaagcta
     6661 taaaatatat caaatatgat cttaagaaaa ctgatttaaa acttttggat cggaggttaa
     6721 taaatatctg aattgcaaaa gaaaaaaata taagaaagct aagatcaacg ggagggaaag
     6781 atagtccgaa taaaactcct gcaagcggta taagtatttt aaacaaccat ccggaatcag
     6841 gtatatcttt atttctgacc aacatgatta ttaagttaat gcttcaggat cttttattat
     6901 cccgaatttt ttattaaata aattccacaa ataaattata agcattgcaa acaaaattcc
     6961 aatcactgcg cctgccataa tatcaaaagg ataatgcact ccgcacatta ctcttgataa
     7021 agcaaccaaa gccgcgccgg tgaagagaaa gcctttcatc cgtggataaa aatttgagaa
     7081 tagataagca gctgcaaaat tatttactgc atgatttgaa gggaatgaaa atgactcgga
     7141 acagttgatc agcaaattca catctgtcag gacatggcag ggtcttgttc tctgaaaata
     7201 tgatttcaat atattattcg aaaactggtc tgcaagtaat atcagcagca gtattaaaat
     7261 tgccgatact tttccttttc ttcctccttt aaaaaacaag taaagccaga tcaggacata
     7321 aaatataaac cagtgatctc tttcggtaat gaacggcatt aacctgtcag tgaagggatt
     7381 tgccagtatg ttatttataa aataaaataa tgcagcatct atattttgta aaatctccaa
     7441 caataatatt tatttatata aaagaactgt tgcataagcc acgcagcctt cttccctgcc
     7501 gacaaatccg attccttcag atgtagttgc tttaattgag atctcatttg tcaagattat
     7561 atctgaaatg ttctttttca tctgatcggt gtatttataa atctttggtt tttcgagtac
     7621 aagcgtgcag tcaatatttg cgatctccca tcctttactt ttgataagtt caaatgatct
     7681 cttcagcaga acaatactgg agatgttttt atattcagga tcagtatccg ggaactgatg
     7741 tccaatgtct ttgaatccag ccgcgccaag tagagaatcg caaacagcat gcaggagaac
     7801 gtcagcatca gaatgacctg caagaccttt atcaaaagga atttcaactc caccgagaat
     7861 cagctttctt tcatttgaaa atctgtgaac atcataacca aagccagttc tgaagttaat
     7921 tttattcaaa aataaattaa tatatgataa tgaaaattgg taataaactt atcggttttt
     7981 aatatttaaa acaatttaac aatatttact tttccatttg atataaatca ttttatccaa
     8041 aagaattgtt caatgatatt catttttaag tggaaaatga aatctaaaga tagtattttg
     8101 aaagtcttaa aatttaccgc ccgggtggcg gaactggtag acgcgcagga cttaaaatcc
     8161 tgttgactgc aaggtcagta tcggttcgat tccgatcccg ggcacatggg ctaaacctgc
     8221 ttagtaataa gcaggttttt ttttggaatc aaatatttat aatttatccg gaattgaccg
     8281 aaacacaaga tttacagact gccggtattt tgtgattagg aataatgtat aaaaaatctc
     8341 aagacttcag acatgaaatt gaaataaaaa aatattattc gatctttaag caaagttcta
     8401 atgcttcatc aatggtcatt tccattcctt cttttctgca tagattgaat ttttcttctc
     8461 caagattttt caatattatt tctctgtcct catcgttcaa tgccagtgct ttgataatag
     8521 ctttatactt gtcgtctttt gaaagctttt ctataaagcc aaaaagttta aatgatcttt
     8581 cgaattcatt tttcttaaag tacaatcttc caaaacctgc aagcagatcc agcataaaaa
     8641 actctcctcc gggtttttct ctttcactaa tatattcact ataaatatct attgcctgtt
     8701 gatacctttc cgtttctata tataaaaatc ctaggtggat cctgatcgga aagagattca
     8761 ttttatatcc ccattcaatt gacatatcat aagcctccag aagaagatat tccgcttctt
     8821 caaaattttt attttcataa tataatttta aagcagcgag gtgtacaaga tttactgaaa
     8881 taagataacg gtcatcaatc cttctggaaa tgattagact ttcatctgaa catactgatg
     8941 ccttttttat gtcacctgtt ctcctagaat gagtaacact taaactcaaa agagcctgag
     9001 cttttaaatg atcactgtta atttcacgag caatttcaag agcttcctct ttcaataaaa
     9061 tagatctttc aaagtcgccg agatttccta caggaaaaga taagttatac aacgaatctg
     9121 ctttactttc cattgagctt atctcattac ttaatgcaag tgcttcttca ttatattgga
     9181 cagcacttgc attatcaagc tcagtgtagg atttcaaagc aagaatattt aaagatttta
     9241 atattccttc tttatcattt atttcgcggt acagctcaag tgcttcattt gaaaatttaa
     9301 gaaggtcatc aaattttccc agttcataac acaatcttga gattctgttt atcagatctg
     9361 cctttaattt tttatcattt aaatttatag tgttgtggat cttcattaat gtctcatatc
     9421 cttcctgcag ataaccttta ttcagccaga agtcataaac atttaatacc agttttactg
     9481 catcgacaga attattttca attgcccact gaatatttct tcttatatta tcgatctcag
     9541 tttccatcac attcagccat tcaaactgac ccatagcttt atgtttttct aacgatgaaa
     9601 aatcgataaa ataattcagg tgtttttgat attcttcttt tctgccgtca agtttttcca
     9661 gggcatagta tttaattgac tcaagaatac cgtatcttcc aattcctttt acttctttaa
     9721 aagtgattag tgatttatca tatagacttg tcatcagatc taatacttca tacatatcaa
     9781 ttttgtcatc actgcagatc tcttcagccg cttcaagtgt ccagccaccc ataaatatag
     9841 aaagtctttg cagcagttcc tgctcatcag cttccagaag atcatagctc cagtcaatca
     9901 ttgcttttag tgtcttttgt ctgggaagtg ctgtagatcc acctcctgtt aacagtttaa
     9961 atctgttgtc aagacggtca agaattttat ccacagttaa tacattgatc cttttagctg
    10021 caagctcaat agcaagtgga attccgtcga gtcttttaca aagctccgca acgtaataaa
    10081 tattatcttc tgttaaattg aaatttgaat taatagaatt tgcaatttca agaaagaatt
    10141 ttaccgattc atattcagaa agagatttaa aagttttgtt tttaatgttt tcaggaattg
    10201 acagcggagg aattctgtaa atggtctctc cgggtatatt gaatgacatt ctgcttgtag
    10261 aaataatctt tagcttggga catgaagaaa gcaattccat agcgatacta ctgcatttat
    10321 caagaaggtg ttcacaattg tcaaataaga gaataatgtg ttttcccttt aaatattctt
    10381 tcaaagtttc caataatttt tttcccggtt cttcctttac attcagcact gtagtaattt
    10441 cactgacaat aagttcggga tctgtaaccg gcgaaaattc aatcagccaa actccgtttt
    10501 caaactcgtc aaggacaccc cctaaaattt ctacagctaa tctggtctta cctgttcctc
    10561 ccgatccaat aagagaaagc attctgatat ttgcaaataa ctctttaatt tcttcaatct
    10621 cttttttacg gccaatgaat tttgaaacag aaacaggtaa attgttacgt ctggcatctt
    10681 ctgtcttgag aggaggaaaa tcatcttcga gaccttctgc aacgatctga tataaatgct
    10741 caggtacaat aatatctttt agttttctta taccaaaatc cttaaacgaa aagtctttta
    10801 aattcaggtc tttcaatgta tcaaagacta tctgtgatac aataatctga cctccgtaag
    10861 caattgacat tattctctga gatctgctga gggtaacata acctgcgtag tcatctttaa
    10921 taaattcagc ctctccgatg tgaataccca ttcttacttt aatttcagaa ccgtcccatt
    10981 cattttcttt taatttgatc tgtgttttta ttgctgccct gactgcattt acaggacttt
    11041 caaaagcaca gcaaaatgag tcaccaacta tcttaaaaac aaatccatct tttgaatcaa
    11101 ttacttcata caaaatttca tgatgctttt caagagatgc aatgtatttg tcgtgattct
    11161 cctgagccag cttggtactg ccctgaatat cagtgaaaag aaaagtaacc gtacctgacg
    11221 gtattttatc catttaaata agtaaaaaat taaaattaaa tctgaccctc aatttcaggt
    11281 tactatcaag taaaatgcaa agataatgta aatgtgtaaa ttaattataa aatttcatta
    11341 aataacttaa tatatttagc ttaatttgat ttcccgaaaa gatgaatcag atataataaa
    11401 tttattgcat atagaattta ttaaacatat tttgctaata ttattttttt ataacagaat
    11461 ataaatctta accccctttt aaaaatttca attaaatgac aatgaaatat tttttaaaat
    11521 tcgctttgtt ggtgatatta tttttaagtt ttaataatat tacaatttct gaagtgatat
    11581 ctgaaaatgg gaaaaagagt tctagtcagg attccagatt agtaaatcct ctttacgaac
    11641 tcagagcaaa agacctgatc agcagttcca acccattaat agatccaaat atttattatt
    11701 ttgatcttgt tatatcacac acaaatttac ttgaatcagg tccttttgaa tatgctgcag
    11761 gacagtatac tctttcattt aactggctca tcagattgtt cggaccactt acttatgaga
    11821 tcgttccaaa cacttcagat ttcacaaatc cggatgcact tcccgtaaat ccaagagtgg
    11881 tagggaacaa actaattctg gacagaaatc ctaatttaat tgcaggacaa ggtcctataa
    11941 tttcacccaa tatagaagga acccgaattg taagaatgaa agtaacaaca tctgtacctg
    12001 attttttgaa tattcctttg aaacttaagt ggataagtaa tccaacggat tctaatacta
    12061 ctgctgttta tgcttttatt gaagattcag taaccaacat ctcatccggc ggaacattgc
    12121 ttattgactc aggttcagtt gcagcattaa atctcaaagc tgcaattagc ggactttata
    12181 atcaaacgag taatcaatta agagcccgag atactctcag ggtatttcaa aattccatta
    12241 cttctccata caatattgta gattccacca gaactataat agattcgatt tcttttacgg
    12301 gagtttgtaa ttttatgaat gcaacttcag gcaaatatta tacagtaata aaatataaaa
    12361 atggtcttga gacctggagc agatcaggtg gtgactcttt aaaaagagga agtcaggtaa
    12421 gttatgattt taccgatgac agtacaaaag cttttggaaa taatctcgag aaaaagggga
    12481 tcttgtattg tatttacagc ggcgatgtta atcagaatgg aatcatagat attgccgatc
    12541 tgactctggt agataatggt atatttaatt tcatttcagg atactctata ctggatatta
    12601 atggtgataa ctttgttgat ctgacagatt cacagcttac tgataataat gcttataatt
    12661 ttgtaactgt tataaagccc tgatctgttt gattttaaaa tttaaaaaag ccggatatat
    12721 ttttattccg gcttttttta ttaaatcaga taaaagctga tgtataattt ttaggaatta
    12781 tatttattca tttttatcac tgcattccag atccaatata ctgatcgttt tatcataaaa
    12841 ttcatttgcc ctttccggag agctgccaat acaaacaact ccaagttttc caaattcaga
    12901 taatgccccg atcagatgaa atacaacgcc ttcctgagta gctccgtcat acattagtcc
    12961 gtgaaacatc gctatatcaa tgagttcact tggtgtaatg ccaatataat tatcactctg
    13021 tacattgtct gaagcaaaat agtaacgcgg gtttccgctt gcagtaatat aaactccttc
    13081 ttcagaatta tattttccat atgtcagata ttcgatcatc atgaaaggat gtgtagtacc
    13141 gccttttcgc aggtttattt caattgcata atgtatccac tttccatcat cctgttttac
    13201 tgaaataaag tcaactgcaa accttccaag aacacctttt ttcgaaagca gatccgctac
    13261 ctttttccct atcactccca gctctgatgc atattccttg tcagcaggaa aaattgctcc
    13321 aagaaaaatc tgaccatcat caccaccaag aagctggtcg tgagtactgt caattctgat
    13381 tttatctgat gcaccaatta cacattgcac agaaggagaa actttaattt caccttccaa
    13441 aaattcttca acaatacctt ccatttcaat aaacttctca aaaaacagat caaggcttac
    13501 atcatgtgaa acaggtcttg tatattgata aagtgtatca aatatttttg tttcaagaaa
    13561 ctcatctatt tcgagatcag gatatctgta aattgcattt ccatctccgc tgaatccgtc
    13621 attcatttta ataactgctt ttctcagacc cggatctttt cttttaagac gggcaagtga
    13681 acggacaatt tcctctttcg attttagatc ctcatctcca tccggcattt ctataccgca
    13741 taatctaaaa gtttttcgtg caccggattt tgaaccttca taaaacattg aaggatcagt
    13801 accaaataat ggtatctcca gttttacagc aagagacttt tccaatggtg ttatattata
    13861 acatgtcaga tgagtagaag ctttatcagt taaaagactt tttattcttt caattaatct
    13921 tggacgctcc aggatctttt cagtaagtgg ttttcgggaa agatcataac aactgaacat
    13981 tgtaagacgc tttctggcat gactgcctgt gatcccctga agcaaatgaa gataataatc
    14041 tattataact tccgatagtg gcatacttga tacatagatt atttttgtca tcggcatacg
    14101 taaaagcatc agaagacaaa gcaaacgctc ttcataatgt atcgatcctt ttatctttga
    14161 aagtatctcc atgtccagag ataatgaagg tataattaca actgttcttg gagcgtactt
    14221 atcagggaat atattttcat actgatatgc aagtccttcc tgtatctttt caaacaggat
    14281 ctgttcttct actgtaccag gcttcggacc gaaatctgaa ttttcaaaca actttcggtt
    14341 taagttaaaa tcaatattca tttaaaaatt aattttttga ttttaataaa aaacaaataa
    14401 ttcaaaatgg aaaatttttc agtaaattta acaaaataaa ttaataaact taattccttt
    14461 ttacgggttc gtaattttta tttatctcaa tttcatttgc ataaggactt ctccaaccca
    14521 tttcaaatac atttctcaga tcattattta caagtttgtt ttttataata atggacacat
    14581 ttcttgagtg acgaaaatat cccgattccc agttagaagt actgatccag gaaacatcat
    14641 tatctgagat catatatttg gaatgatcga ctcttgcaaa cggaataaat ccttcactgt
    14701 aaagaggaat tgaaattatt tttacttcaa tatttttaat ctgtgaaaat tcttttataa
    14761 aatcctgaga actgcctccg atctcccagt cgggtaatat catttttatc tgtacatttc
    14821 tgccggcagc ttcttttaat gaatttgcaa ttctatcaaa ttggactgta tcatatctgt
    14881 ctttatctga gaaagaatat atctgaatca aaagattttc ctttgcagcc gaaataatat
    14941 taataagttc atcttcttca ttacttactc ctgatatatt ataatcaggc gggctgaaag
    15001 ccggatacaa aattatttca ccatattttt cagattttat tgtcacaggt ttttcagaat
    15061 tcacgatagc aactggtctt tgatttacaa gtacggaaat atttccatca cagagattcc
    15121 agtctgtttc aaacaattcc gtaaaagtct gagcaagatt tttatccttt acacgcagac
    15181 caatctcatg aatatgttta agagctctcc agtcaaaatt ctgacttcca agaaaaatgt
    15241 tttcgttatc tacaataaaa aactttgcat gcataacacc tccgccaaga tttccaaacg
    15301 ggatcttctt aattgtgatg ttattaattc cttcaagctt atctactgat ttatcatttc
    15361 gtgaataaaa ggaagaatca acaatgatcc tgatacggac tcctctctct gcagcatttt
    15421 tgagagatcc cattattatt tccagcggtt catttgtttc atcagcaaaa taaaaagttt
    15481 caatatcgac agacactgat gcatcatcga tcattttttt ccagacatcc ggagttttag
    15541 caagagttga attttcatat actgtttcga cgggtgtact ttcgacaatc tcaaattcat
    15601 tttgagcata tagaatattg acagaaataa aaaagaaaag taaaatcttt tttaatttac
    15661 taatcaggat catacttctc tttttaagat ctttattatt tactaagcgc atatcagatt
    15721 actaaaatat tcataagttt ttcaatttga gaaactttaa gctcttcttt attttctctg
    15781 taaatttcca gtaagtttct aagaattctt aagaggatct cattgtcggt agatttattt
    15841 aaatacggga tctgatcaaa atctgatttc tgtattccaa tattttttgc aaacctttct
    15901 gcttcagatc ctgaaataat tattccttca ttaaaaggat caatataata ttcagcattt
    15961 ttattttcat acttcagaag aaagtgaccc ggcatattaa ttccataaat gggtaagttc
    16021 agctttttgg caacgatgag ataaattaca gatagagtta tcggtattcc tgttttatta
    16081 tcaatcactt tatttataaa actgttgtca ggatcgtaaa aattttcttt gttaccttta
    16141 aattttaatt caccaaacaa atactcatta attgtttcaa gaaggtcaag aggataatca
    16201 ggttcaactt ttcgaaattt attgatcact cgttgtttga tttctgagac atattcatcc
    16261 gtaatttttt catatttctt catatccact aaaggatatc cataagaagc aattaagaaa
    16321 atacttttct cgagatactg cttactctga gaatgcttaa gtttgttgaa ctgatattca
    16381 aggttggtaa aattcagaat tgagattatt tcgtttgctc ttttctttat aagtaaattt
    16441 tcatcgttta aatatccttt cagaaaatct tcagacaaaa tcccataaga aaggaacctg
    16501 tcttttacac tcttataaat attgacatct tcatcatcca ttaattttat tagatgatca
    16561 aattctttgt ttggggtaat tgttacatca ctatctgttg tcattttaaa atctccttta
    16621 ctttaacttt ttttaagagg aacattccta ttatgataaa aagtccgacc gaaagaattg
    16681 caagtttttg tgaaccggat atccatgaaa cgagtccgaa agtcagagga ccaatcaagg
    16741 tagatgtttt atccatcaaa gcataaaacc cgaaaaactc agttttcata tccagtggtg
    16801 taagatatgt catcaatgcc ctggatagac tttgagtaga accaagaaat aatccggcaa
    16861 atcctccaat aataaaaaat gaattcttat catttgtaac aaatacagcg attgtcagga
    16921 gagtccagaa tatcagattt atatttaatg attttattat tcccattttg tcacctatca
    16981 gtacaaacag atatgaaccg atcatagccg ttatctgaac gatcagaaaa aagattgcaa
    17041 gttcggtaat cgaaaatccc agtgtagaac ttgcatagat tcctgaaaaa aatataatcg
    17101 tatttacagc atcaatatag aaaaaaaatg aaatgagaaa gtttttaaga tctttgaaat
    17161 tatttagatt cttaagtgta ttaaaaactt tattaaatcc ataagctata taatttattt
    17221 ttaccgggcc attacttttt ttctcttctt ttaggaaaag gaataacggc attgaaaaga
    17281 tcagaaagaa tcctgcgcag ataacgaata gaatatttgg gtcgtcttta aaagggaata
    17341 caagcagtac agagattaca gagcctacat aacctgtagc ataaccgtaa ccggatactt
    17401 tgttgtaatc ttttggttcg gagatctcgg aaataaatgc atcgtaaaac gtaagtcctg
    17461 tttgaaaacc tatattggaa agaataaata acacgagtgc aaagaagatt gtaccttcac
    17521 cggtaaaata cattaaagaa gtagaaatga cacagagtat ggtaaatgcg aaaaggaatt
    17581 tctttttgtt gctggagtaa tcggcaatag caccgaatac cggatttaaa actgcagaga
    17641 tgatcattga gatattaata cctattgacc agtaaagatc accgatactt tcaccggaaa
    17701 caacgacctc tttaaagaat acagcaaata caaaagctac tattattaca gcataagaag
    17761 aattaccaaa atcaaaaagt gaccagacta agacctgaga tttattcttc aaaaataatt
    17821 tattttgaaa ttaacaacta aacttaaatt aatttgtatt tttaatgtgt ggcttagcgt
    17881 ttttcttttt catatagtcc ttcttttccg gatctatctt ttctcttttg ctatctgatt
    17941 ttttattgtt ttttttgttt ttaccttcag ttgtaaccaa acctctaccg gtctttacaa
    18001 tatgttcatc ccatcttatg atggattctc ac
//
LOCUS       DAORVQ010000047        17748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1326_length_17748_cov_6.801616, whole genome shotgun sequence.
ACCESSION   DAORVQ010000047 DAORVQ010000000
VERSION     DAORVQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 17748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17748
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_17748_cov_6.801616"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLX80_05710"
     CDS             <1..240
                     /locus_tag="PLX80_05710"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20325.1"
                     /translation="NPFNPKTIINYKCSMYNDVSLKVFDVLGNEVDELVNEKQNAGSY
                     SVIFDGSNFPSGVYFYKLDLGEGEFVETRRMVLLK"
     gene            531..2549
                     /locus_tag="PLX80_05715"
     CDS             531..2549
                     /locus_tag="PLX80_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HQY20326.1"
                     /translation="MESQVTAEIKRRVMNFDRKEYDDETLLSLYKAILLPRLIEEKML
                     LLLRKGKISKWFSGIGQEAISVGVGMALDKDEYILPLHRNLGVFTTKGIPLAKLFKQL
                     QGKYNGFSKGRERSFHFGTNEYHIIGMISHLGPQLAIADGIGLAALLKNEKKVTAVFS
                     GDGGTSEGDFHESLNVAAVWNLPVIFVIENNGYGLSTPLNEQYKCGDLSDKGVGYGME
                     ALQIDGNNILAVYDTIKTYAESMKNDPRPLLLECKTFRMRGHEEASGIKYVPKELLEE
                     WAKKDPVSNYESYLIEEGILDDEKIEALRNEMKLNINTALDEAMDEPFVTPDTESELK
                     DIYAPFEQKVILPDSDKTSPKRLVDAVSDGLKLSLRKYSDLVIMGQDIAEYGGVFKVT
                     ENFYKEFGKERIRNTPICESAIIGAALGLSVKGIKSVVEMQFADFITSGFNPVVNNLA
                     KIHYRWGQNADVVIRMPCGAGVGAGPFHSQSNEAWFFHTPGLKILYPSNPYDAKGMIA
                     AAIEDPNPYLFFEHKAMYRSIAMDIPDDYYTVEVGKAKLVREGDDVTIVTYGMGVHWA
                     ENLLKANEDISADLIDLRTLVPWDKATVEESVKKTGKVIVMHEDTLTGGIGAEIAAYI
                     SEHLFKYLDAPVMRSGSLDTPVPFSNSLEENFFQKDVFEKKLKDLVDY"
     gene            2610..3077
                     /locus_tag="PLX80_05720"
     CDS             2610..3077
                     /locus_tag="PLX80_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQY20327.1"
                     /translation="MSKILLILSAIFILSNISGKAYSQNTEYDSLRAAELGADDYGMK
                     QYVIAFLKAGKVKSESQEKAQELQLEHLRNITKMAEEGVLVLAGPFLDDTEIRGIYIF
                     DVKTVGEAQRLTETDPAIKAGVLEMELHPWYGSAALMELTKIHGTIQKKSITD"
     assembly_gap    3118..3217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3230..4654)
                     /locus_tag="PLX80_05725"
     CDS             complement(3230..4654)
                     /locus_tag="PLX80_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQY20328.1"
                     /translation="MSDKNPSEVHSSGLLTKLGLMTAILIVISSMIGSGIFKKAAPMA
                     MDLQSGGLLLACWIIAGLITLIGAVTNAEIAGLIAAPGGQYVYFKEMYGKAFAFIYGW
                     SCFSVIQSASIASIAYVFAESVNAVLPLPHLSASMESFNILGIFYPFASFGVKALTIA
                     TIIFLTTANYLGVIFGGYINNIFTILKVIGILTIIFLGLTLSGGSVDNVAPLGVNPSA
                     EYGTSLGLFGAMFAAMLGAFWAYDGWNNIGYLGGEVKNPKKNIPIALCTGVLIVIGVY
                     LLTNFVFLYVIPVSEIIEIAQKENTIIAVEVMRKFLGFGGAFFISILIMVSTFGTTNG
                     TILASSRIYFAMSRDKLFFKPAGNVHPKFKTPYTSLIIQGVWACLLVLSGTFDQLTDM
                     VIFASFIFYGAGAFGVFVLRRKMKDHHRPYKTLGYPILPLIFVLFCITLVVVTIIQNP
                     RDAGLGLLLVLSGIPFYIFWNRKK"
     gene            complement(4679..5710)
                     /locus_tag="PLX80_05730"
     CDS             complement(4679..5710)
                     /locus_tag="PLX80_05730"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQY20329.1"
                     /translation="MKIGILTSGGDCPGLNAVIRAIVRKSDQLGFETIGIIDGWKGIF
                     DENYIQLDLNSVAGIINKGGTILGTSRFSPFEKKNGMDKLLNKISRKKIDAIIAIGGE
                     GSMHIAQMAYEAGINIVGVPKTIDNDISGTDYTFGFDTAVGIATEAIDRLHTTAESHH
                     RIMILEVMGRHAGWIALYSGLAGGADVVVIPEVTTKISQVIKVIKSRYNRGKRFSIIV
                     IAEGAKFDEKSMKLKKENKKLDDFGRERLGGICTFLADEIERRTGFECRATILGHVQR
                     GGMPSAFDRVLGTRLGIYAVEMAYEKKFGRMAALQGNEIVDIPITEAIGKLKTVDKDL
                     YEVAQVFFK"
     gene            complement(5820..8273)
                     /locus_tag="PLX80_05735"
     CDS             complement(5820..8273)
                     /locus_tag="PLX80_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20330.1"
                     /translation="MAKKNILNRTNHGTTKPAYYAGIFIVAMSTLLLEFTLTRVLSVS
                     LWYHFAFMIISVALLGFGISGVVLALSSRIEKIESDKLLSILSILYGLSVLFSFISMN
                     KIPFDPFSLLSESIQLLYLPVYYLLITLPFFFAGLVISVLLVRYKKDVSKLYFYDLTG
                     AGISCILFIILIPLTGGSGAVAVVAAMGFLAAVLFSYEKYKTISLISVILMALSLTFL
                     INSEERLPISITENKIYGNYVKDRPDLKVRTDWNIFSKIDVMKDEDKSEDGYDIYLAI
                     IDAGNATTNIPNVTSLPPKTKPADASNLAYVLKDSAEKVFIIGSSGGGEILAGLYHNA
                     KDIKAVEINGILNDYLESSLQYWTGPLVKNNKSVNLITDDARNILNSKRIVYDVIISA
                     HTISASAMSSGAMSMVENYILTKEAVKEYLHHLNNKGGMLYISRPETQIPKLITTLKE
                     ARLENSRGLDDSKNCFIIFRRPPNDFEGEKSFLAGVLYKKDGFSAEEILKIREESSSL
                     SLDLMYDPLSERESFYKTLIESDNFRSLAGSEEGITESTLEPATDDKPFFDQNIGFGN
                     LTMKGISETFSQDDKAILALKDKPVAETSLIVILVQTLLTAFAFLILPLILAKKKLES
                     KATGKTFLIYFAAIGLGYIMIQISLIQKFTLLLGQPVYTILTVISSMLIFSGIGSRFS
                     DSFLKNNKYKIQIIFFLIALIAILIGVFNSYLFSAMARFDLIYRVLISILIIAPLGFL
                     MGMPFPMGMEKIDNSEKGLAAFSWAINGFFSVIGSVMAIIFAMTMGFRFVFIFVSVCY
                     LIAMLMIIFRNKAMKLKTV"
     gene            complement(8281..9105)
                     /gene="kdsA"
                     /locus_tag="PLX80_05740"
     CDS             complement(8281..9105)
                     /gene="kdsA"
                     /locus_tag="PLX80_05740"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQY20331.1"
                     /translation="MLPSYFKKELNIKNPENFLLISGPCVVESKQVIFKTCEALKKLS
                     EKYKLPFIFKSSFIKANRTSTSSFRTLGIDESLNILAMAKKEFDVPILTDIHSELDAE
                     LASEVADVLQIPAFLCRQTELLEAAGETGKIVNIKKGQFMAPADMIFQAKKVEATGNK
                     KILLTERGTTFGYNNLVVDMRSLVIMKKSGYPVIFDATHSVQMPSGNKGVSGGNPEFI
                     APLSRAAAAVGVDGFFIETHPDPKKALSDADSMLKLDKMESLLKELISVHKLNFKS"
     gene            complement(9111..9698)
                     /locus_tag="PLX80_05745"
     CDS             complement(9111..9698)
                     /locus_tag="PLX80_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQY20332.1"
                     /translation="MTPKRFNKFSHVISKRQKFLTLVLENIHDQHNVSAIFRSAEAAG
                     IDKIYLIYNTNKFPKIGKTSSASAKKWITTEKFESVKDCFEELKKNKFRIYSTHMNEE
                     KKKNLNLYDLDLTKKVAIVIGNEHTGVSDEVKEMSDKNFLIPMFGLIQSLNVSVAAAV
                     CVYEALRQRLAKGMYERSGYTKAELKEKLTDYLNR"
     gene            9769..10407
                     /locus_tag="PLX80_05750"
     CDS             9769..10407
                     /locus_tag="PLX80_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20333.1"
                     /translation="MKKIYVPLIALFLLTFILTSVSYSQIKYTKPEPRNIWGFGFSYS
                     ERGFGPNASLYVPIGKTTDVFFNVLFSNVTDSRETERTDPFGNTFVINKINRVFMMPL
                     SIGLRKELFTDDIEGEFVPIFNVGISPTLIFLNPYDRDFFSALGYTTTQFALGGFTGL
                     GVSFRQSKNMSLNFNLSYYYISTLGDPVQSLNGSDINNVGGLQLGFAINFMN"
     gene            10616..11062
                     /locus_tag="PLX80_05755"
     CDS             10616..11062
                     /locus_tag="PLX80_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HQY20334.1"
                     /translation="MLILFGVITVKGIAIAKNIHKFADGPEGFIIDKISEKLDLNASQ
                     KTQVERIKAQIKEKMESQKPDRESMMKEFSEEFTKSTLDKNSILDQMKKNESKREEMK
                     TFMIDKLVEFHAILTPEQRVKAVELMFEFKEMKDKHQPRGPGNFMK"
     gene            11126..11812
                     /locus_tag="PLX80_05760"
     CDS             11126..11812
                     /locus_tag="PLX80_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY20335.1"
                     /translation="MKKILIIDDDKKLSDLLIDYLKKYSFEPVSVENPLDGLSELEKI
                     NYDFVILDIMMPDMDGFETLKEIRKTKDIPVIMLTARGETTDKIVGLELGADDYLSKP
                     FEPRELLARIQSVFRRTKNKRNGNSIFDFKELFIDPSSRKTLLQGKTLDLTSMEFDIL
                     LLFAENNGKVLSRDYLMQKVKGTGWNYNDRSIDVMVSRLRGKLHKKKLEFIKTVQSVG
                     YIFFAEDEED"
     gene            11799..13088
                     /locus_tag="PLX80_05765"
     CDS             11799..13088
                     /locus_tag="PLX80_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQY20336.1"
                     /translation="MKKIKFPIFLKLLLLFTVFIIIVNLTAGFIAKLSLGVDPAIMIN
                     KYIMSLNEYIIKDIGNPPDIKTAEEIAKKKNLDIRIETDELIWSNSERIPDLKELRQF
                     PEFREGAPSFPMRFRGGPLFFTKINNGYIIISPAKHIEFIEKEKAVLLIIVAISILTL
                     ALYFALRWIFGPIKSISKDIEEISRGNLNTRIIINRRDELGRLAESINEMKEKISNMI
                     NSKESLLIDVSHELRSPLTRIKLACEFIDDEKLRIKINEDVNELESMISGLLDTYRNE
                     NTGVSLQKENIDLTGLVINVVTKFNNDRVNYISEMNECFINADKIGIETVLRNVIDNS
                     LKYSEDKPVTVKLSEHKDSAKITVKDEGKGIDKSELEKIFEPFYRTDKSRNKKIQGYG
                     LGLSLVKKILDGHGFQFEIKSEPGAGSEFVIHFHVVR"
     gene            13414..16308
                     /locus_tag="PLX80_05770"
     CDS             13414..16308
                     /locus_tag="PLX80_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQY20337.1"
                     /translation="MRSYLKIAIGIFIIFISSIDSYSQFVNFGRNKVQYADFEWSVLS
                     TEHFKIYYYQDAKELAEIGANYAEESYREHQEKFKHSLVDTVPMIFYTSPLHFKQTNT
                     TPGLIPDGVGGFFEFIKGRVVIPFEGSLSQFRHVIKHELVHVFMMSKIGNVQKTHRKS
                     IGAAPPLWFTEGLAEYWSTQWDTQADMVLKDAVLTGYLPGLSNWERVYGTYLMYKLGQ
                     KVLEYIALNFGEDKILQLIDNFWVDENFSNVMQYTLGVDYAQFDKNFLYWLEKQYYPE
                     YKDLDDPSAVSENIYTESFAHKPTYSNANGNQEVFFIGNKTGFTSIFKLNLRKLSDGA
                     DLVIGGESSDEFEQFHFFRTGIDVSSKGNLAFITQKGNADALHILNTRTNEVIADYSF
                     PDIVSIGSPSWSHDGNKITFPATDFGGINDLYILDLLTNELTRLTNDYYDDRDPDFSP
                     DGDHIVFSSDRTTFGKKKAYNLFIYEIKTGRIDYLTIGDQIDYAPNFSEDGTKIIFTS
                     DINGPQNIWMIDLKKAITSEKSESGTNALEDKSGINYDDLEMRQITYFISAAYDPKWS
                     GKDRIVFSSFEKGSISIKMLRSIQERFDSSKNVVKTNIKEKGENWTADKIKSYTNKNN
                     VPYKKDFSLDIATTALTVDPVFGANTGGIISLSDLLGNEKYYFLIFNNSNGSEDFWNS
                     FNIAISKISIENRLNYAYGIYHLSGRRYDLGEFNSSFYERQYGGYLSLAYPLSFFRRL
                     EMTTSLSQSTRSYDLFDNTRSLLLSNFISYVKDNTLWNYTGPMDGERLNLTLGYTTDI
                     ANSNINYYSLFFDFRKYFRLGGPTALALRTQFFMNEGKEERRFFMGGSWSLRGWPLNS
                     IRGSKLWQTNLELRFPLLNVIALRFPLGIGFDFPGIQGAVYFDAGNAWDRNYTNVYTN
                     TYTRGSIGAGIRLNALGLVVLRYDFGKRIEENFTKLQGDFYQNFYFGYNF"
     gene            complement(16326..16976)
                     /locus_tag="PLX80_05775"
     CDS             complement(16326..16976)
                     /locus_tag="PLX80_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQY20338.1"
                     /translation="MADEKISGVIQTETSSLIKTYDGEGKNIILAITGTSGAIYGLRI
                     LRALLINDFNVDLILSEYASYTMYKECGIDLKPNVIQTLFPEILILKSTVSFHNNLDL
                     KSEIFTNKFNCHGMVIAPCSMGILAGIANGECKTLMEKSADYGLSYSKPLVMVPRETP
                     LNKIHLSNMIKVIEAGGKIVPAMPSFEYNPGDFNDLADHIAGKVMDILNMNNHPPA"
     gene            complement(16985..>17748)
                     /locus_tag="PLX80_05780"
     CDS             complement(16985..>17748)
                     /locus_tag="PLX80_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20339.1"
                     /translation="SLKRNKTSKFKFPEFKKASELFTRLLPGSNIELASFRLIRDHIN
                     NSRYLRLKYIPLLIIPVIVVIIGFFSNSAEYLFFESKSGFLRTAFLVISPSVSLTVLL
                     CARMLISNTKILDDSSSDTKWIFDSLPSGNISSYIKGVNSFIYFVMILPMLIFIFLML
                     LFKADTLTIFLNFIYISSSVYFVISIASLFDKTLPFTLESSKYNSATRFLEVLISMVI
                     GVAVFLIQIFIFQNIIFVITATVLFITVSYLLNRN"
BASE COUNT         5832 a         3084 c         3278 g         5454 t
ORIGIN      
        1 aatccattta atccgaagac aattattaat tataaatgtt caatgtacaa tgatgtatca
       61 ttgaaagtat ttgatgtgct tgggaatgaa gttgatgagc ttgtcaatga gaagcagaat
      121 gccggaagct attcggtcat ctttgacgga agtaatttcc cgagcggggt ttatttttat
      181 aaattggatt tgggtgaggg tgagtttgtt gagacaaggc ggatggtgct tttgaaataa
      241 tttcaaatta ctaatttcaa attacaaata aaaaaacaca tagatagaat gactttgcga
      301 acgaagcgaa gcaatctccg gattcacgac aaaatattct tagcatcact cacatcaaat
      361 ctgtgactgc ttcacccaac tatcacgtgt ccgcaaagac ataataagca acagcatttt
      421 caatacttca ataaaatttt acttaaacaa acttcaacac aattttcatt tataatcaaa
      481 ttctcaattg ctattttact cacatcaatt ttaacaacta attcaaatca atggaatcac
      541 aggtcactgc tgaaataaaa agacgcgtta tgaattttga cagaaaagaa tatgacgatg
      601 aaacactcct ttcattatac aaagcaattc tcctcccaag gctgatcgaa gaaaaaatgc
      661 tgctgctgct gagaaaagga aagatatcaa aatggttttc ggggatcgga caggaagcaa
      721 tttcagtagg agtgggcatg gctttggaca aagatgaata catacttccg ctgcacagaa
      781 atctcggtgt ctttacaacc aagggaattc cgcttgcaaa attattcaaa cagctgcagg
      841 gaaagtataa cggattctct aaaggaaggg aaaggtcttt tcacttcggt acaaatgaat
      901 atcacatcat cggaatgatc tctcatctgg gtccgcagct tgcgatcgca gacggtatcg
      961 gacttgctgc tttattgaaa aacgagaaaa aggtaactgc agttttttca ggtgacggcg
     1021 gtacgagcga aggtgacttt catgaatcgc tgaatgtcgc tgctgtatgg aatctgccgg
     1081 taatattcgt gatcgaaaat aacggttacg gactttcaac tccgctcaat gagcagtaca
     1141 aatgcggcga cctttctgac aaaggagtcg gctacggaat ggaagcttta cagatcgacg
     1201 gaaataatat tctcgcagtc tatgatacta taaaaactta tgctgaatcc atgaagaacg
     1261 atccgcgtcc gctgcttctc gaatgtaaga ctttcagaat gagaggacat gaagaagcat
     1321 caggcattaa gtatgtacca aaagaattat tagaagagtg ggcgaagaaa gatcccgtct
     1381 cgaattatga aagttattta atcgaagaag gtattttaga tgatgagaag atcgaagcat
     1441 tgagaaatga aatgaaactg aatattaaca ccgcactcga tgaagcaatg gatgaacctt
     1501 ttgtaactcc tgacacagag tcagagctga aagatattta tgctccattt gagcaaaaag
     1561 taattttacc tgattcagac aagacctctc caaaaagact tgtcgatgct gtgtcagacg
     1621 gattgaaatt gtctctgaga aaatacagcg accttgtgat aatgggtcag gacattgccg
     1681 agtatggcgg tgtattcaaa gtcacagaaa atttttataa agaattcgga aaggaaagga
     1741 tacgcaatac tcccatttgt gaatcggcaa ttatcggcgc agcacttgga ctttctgtaa
     1801 agggaataaa gtcagtagtc gaaatgcagt tcgcagattt tataacaagc ggatttaatc
     1861 ctgtggtaaa taatcttgca aagatacatt accgctgggg acagaatgct gatgtcgtaa
     1921 taagaatgcc gtgcggcgca ggagtgggag cgggaccttt tcattctcag tcaaacgaag
     1981 catggttttt tcatacaccc ggactgaaaa ttctgtatcc gtcaaatccg tatgatgcaa
     2041 aaggaatgat agctgctgcg attgaagatc ctaatcctta tttatttttt gagcataagg
     2101 caatgtacag aagtattgca atggatatcc ctgatgatta ttatacagta gaggtcggga
     2161 aggcaaaact ggtaagggaa ggcgatgatg taactatcgt gacttatgga atgggcgttc
     2221 actgggctga gaatttactg aaagcaaatg aagatatatc ggcggatctg attgatctca
     2281 ggacgttagt tccgtgggac aaagccaccg tcgaagaaag cgtaaagaaa acaggtaagg
     2341 tgatcgtcat gcatgaagac acccttacag gtgggatcgg tgctgagata gccgcttaca
     2401 tttccgaaca tcttttcaaa tatttagacg cacctgtaat gcgcagcgga agtcttgata
     2461 cacctgtgcc gttttcgaat tcacttgaag aaaacttttt tcagaaagat gtttttgaaa
     2521 aaaagctgaa ggatcttgtt gattattaat tgcctgaaat taattttaaa taaaaaattt
     2581 ttaagactaa attaaaacta atttttaata tgagtaaaat tttattaatc ttatcagcaa
     2641 tatttatctt gtcgaatatt tcaggcaaag catattctca aaacactgag tatgattcac
     2701 ttcgtgcggc agagcttggt gcggatgatt acggaatgaa gcaatatgta attgcatttc
     2761 taaaagccgg aaaagtaaaa tctgaaagtc aggaaaaagc tcaggagctg cagcttgaac
     2821 atctgaggaa tattacaaag atggcggaag agggagtgct tgttctggca ggtccgttct
     2881 tagatgatac agagatcaga ggaatttata tttttgatgt gaagacagtc ggggaagcgc
     2941 aaagactcac tgagacagat cctgctataa aagccggcgt tctcgaaatg gaactgcatc
     3001 cgtggtatgg ctcggctgca ctgatggagc tgaccaaaat tcatgggaca attcagaaga
     3061 agagtattac tgactgatct tttatacttc gcttgttccc aaactcatga ttgggaannn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa tgtttccggt tatttcttcc
     3241 tgttccagaa aatataaaaa ggtattccgc tcagcacaag taaaagtccg agacctgcat
     3301 cacgcggatt ctgaatgatc gttacgacca ctaaagtgat acagaataaa acaaatatta
     3361 atggaagaat cggatatcct aaagttttat aaggtctgtg atgatctttc attttcctcc
     3421 taagtacaaa cacaccgaaa gctcccgcac cgtaaaaaat aaacgatgca aaaattacca
     3481 tgtccgtgag ctgatcgaat gttccggaca gtaccagtaa gcaagcccag acgccttgaa
     3541 ttatgagtga agtgtatgga gtcttaaatt ttggatgaac gttccctgcc ggtttaaaaa
     3601 ataatttatc acgggacatt gcaaagtaaa ttctggatga tgcaaggata gtgccgttgg
     3661 ttgttccgaa cgtggagacc attatcaaaa ttgaaataaa aaaagcaccg ccgaatccga
     3721 gaaatttcct catgacctct acagcaatga tcgtattttc cttttgcgca atttcaataa
     3781 tttcacttac aggaatgaca taaagaaaga caaaatttgt aagcagataa actcctatga
     3841 caattaatac tcctgtacaa agtgcgatgg gaatattctt tttaggattc ttaacttcac
     3901 cgccgagata cccgatatta ttccatccgt cgtaagccca gaaggcaccg agcattgcgg
     3961 caaacattgc gccgaagaga ccaagcgaag tgccgtattc cgctgaagga tttactccga
     4021 gtggagcgac attatccact gagcctccac ttaaagtcag tcctaaaaaa attatggtaa
     4081 gaataccgat gactttcaga attgtaaaaa tattatttat atatcctccg aaaataactc
     4141 cgagataatt tgctgtagta aggaatataa ttgtagcaat ggtgagagct tttacaccaa
     4201 agctggcaaa gggatagaag attccgagta tgttaaaact ttccatcgaa gcgctgagat
     4261 gcggaagcgg cagaacggca ttcacggatt cggcaaatac ataagcaata gaagctatgg
     4321 atgcagactg aattacagag aagcatgacc atccgtaaat gaatgcaaat gctttaccgt
     4381 acatctcctt gaaataaaca tactgtccgc ccggagcagc gataagtccg gcgatctctg
     4441 catttgtgac agcacctatt aaagttataa gtccggctat gatccagcat gccagcagca
     4501 aaccgcccga ctgcagatcc attgccattg gcgcggcttt tttgaaaatt cctgagccga
     4561 tcatggagct gataacgatc agaattgcag tcatcagacc gagctttgta agcaatccgc
     4621 ttgaatgaac ttcggatgga tttttatcag acatttttga ttttaaaaaa ataatttttt
     4681 atttaaagaa aacctgtgct acttcgtaaa gatctttatc aacagttttc aattttccga
     4741 tagcttctgt gataggaata tccacaatct catttccctg aagcgcagcc atccttccga
     4801 attttttttc gtaagccatt tcaacggcat agatcccaag ccttgtacca agcactctgt
     4861 caaaagctga cggcataccg cctctctgta catgcccgag gatagtagct ctgcattcaa
     4921 aaccggttct tctttctatt tcgtctgcaa ggaaagtaca gatgcctcca agtctttcac
     4981 ggccaaagtc gtcaagtttt ttattttctt tttttaattt catacttttc tcatcaaatt
     5041 ttgctccttc tgcgatcaca attattgaaa aacgttttcc tctgttatat ctgcttttga
     5101 taacttttat cacctgggaa attttagtgg taacttcagg gataacgact acatcagcac
     5161 caccggctaa tccgctgtat aaagctatcc atccagcatg tcttcccatg acttcaagga
     5221 tcatgatcct gtgatgagat tctgcagtag tatgaagtct gtcgattgct tctgttgcta
     5281 ttccaactgc tgtatcaaaa ccaaatgtat aatctgtacc gctgatatcg ttgtcaattg
     5341 tctttggcac gccgactatg tttatacctg cttcatatgc catctgagca atatgcattg
     5401 aaccttcacc tccgattgca atgattgcat caattttttt tcgtgaaatt ttatttaaca
     5461 gtttatccat gccgttcttt ttttcaaacg gactgaatct tgatgttcca agaattgtac
     5521 cgcctttatt gataatccct gctacactgt tcagatcaag ttgaatataa ttttcatcaa
     5581 agatcccctt ccacccatca ataataccta ttgtttcaaa tccaagctga tcggattttc
     5641 tgacaatagc tcttatgact gcattcagac ccgggcaatc accgccgctt gtaagaattc
     5701 ctattttcaa ttaattattt tttaatttgt ctgatatatt ttcaccgaaa gatcttttac
     5761 agaacactgt taccgcagag tcacctgcac ttcggactct gaggaatgtt gaattaaatt
     5821 tacacggttt ttaatttcat cgctttgttt ctgaaaatta tcataagcat tgcaataaga
     5881 taacacacag atacgaatat aaaaacaaat ctgaacccca ttgtcatagc aaaaattatt
     5941 gccatgaccg aaccaatgac agagaaaaat ccgttgatcg cccatgaaaa tgccgcaaga
     6001 cctttttctg aattatcgat cttttccatt cccatcggaa acggcattcc cattaaaaat
     6061 cctaacggag caattatcag aattgaaatt aaaactctgt atataagatc aaaccttgcc
     6121 attgcactaa acagatatga attaaaaact ccgatcagta tcgcaattaa tgcaataaga
     6181 aaaaatatta tctgaatttt atatttgtta ttctttagaa atgaatccga aaatctgctg
     6241 ccgatccctg agaatataag cattgatgag atcactgtca gtattgtata tacaggctgt
     6301 ccgagcagca aagtaaattt ttgtatcaat gaaatttgaa tcataatata accaagtccg
     6361 atcgctgcaa aatatatcag aaaagttttt cctgtggctt tgctttctaa tttctttttt
     6421 gcgagaatga gagggagaat taaaaatgca aatgcagtaa gaagagtttg aaccaaaatt
     6481 actattaatg aagtctcagc cacaggcttg tctttcagtg ctagtattgc tttatcatcc
     6541 tgtgaaaacg tttccgaaat tcctttcata gtaagatttc caaatccgat attctgatcg
     6601 aagaatggtt tgtcgtcagt tgcaggttca agtgtagatt ccgtaattcc ttcttcactt
     6661 cctgcgagac ttctgaaatt atctgattcg atcagagttt tataaaaact ttcccgttca
     6721 gaaagcggat catacataag atccagacta agcgaactcg actcttccct gatctttaaa
     6781 atttcttcgg cactaaatcc gtccttttta tacaatacac ctgcaagaaa acttttctca
     6841 ccttcgaaat catttggagg tcttctgaat attataaaac aattcttact gtcatcaagt
     6901 ccgcggctgt tttcaagtct tgcttctttt aaagtagtaa ttaatttggg aatctgtgtc
     6961 tcaggtcggg agatgtaaag catacctcct ttattattca gatgatgcag atattctttg
     7021 actgcctctt tcgtgagaat gtaattttct accatactca ttgctccgct ggacattgca
     7081 gaagctgaaa tagtgtgagc tgaaattatc acatcataaa ctatcctttt ggaatttaaa
     7141 atattcctcg catcatcggt tatgagattt acacttttat tattttttac aagcggacct
     7201 gtccagtact gaagtgagct ttctaaatag tcatttaaga ttccgttgat ctctacagct
     7261 ttaatatctt ttgcattatg ataaagtccg gcgagtatct ctcctcctcc tgatgaacct
     7321 attatgaaaa ctttttctgc tgaatctttg agaacgtatg caagattaga tgcatctgcc
     7381 ggttttgtct taggcggaag cgaagttaca ttaggaatat ttgttgtagc atttcccgcg
     7441 tcgataattg caagataaat atcataacca tcctctgatt tatcttcatc cttcattacg
     7501 tcaatctttg aaaaaatatt ccagtcggtt cttactttca gatcgggtct gtctttaaca
     7561 taattaccat aaattttatt ttccgtaata ctgatcggaa gtctctcttc ggaatttatc
     7621 agaaatgtca gagacaatgc cattaagata actgaaataa gtgaaatggt tttatatttt
     7681 tcatatgaaa ataaaactgc agcaagaaat cccatcgctg caactacagc aactgcaccg
     7741 cttccgccgg tcaatggtat cagaataata aatagaatac atgaaattcc tgcacctgtc
     7801 agatcataaa aatataattt agaaacatct tttttatatc tgaccagaag aaccgaaatc
     7861 acaagtcctg caaaaaagaa cggcagtgtg ataagaagat aatacaccgg aagatataaa
     7921 agctgaatag attccgaaag cagactaaaa ggatcgaatg gaattttatt catcgagata
     7981 aaagaaaaca aaacactaag gccatacaat atggataata tgctgagcag cttatcggat
     8041 tctatctttt ctatcctgct gcttaaagca agcactacac cgctaatccc gaatccaagc
     8101 aatgcgacac tgatgatcat aaatgcaaag tgataccata gtgaaactga caatacacgg
     8161 gtcagagtga attcgagaag taatgttgac attgcaacta tgaatattcc tgcatagtaa
     8221 gccggttttg tagtgccgtg attagtacgg tttaaaatat ttttttttgc caaacttatt
     8281 ttaagattta aaattcagtt tatgaacaga gattagttct tttaaaagac tttccatttt
     8341 atctaatttc aacatgctgt cagcatcact cagagctttc tttggatcag gatgtgtctc
     8401 aataaaaaat ccgtcaactc caaccgctgc cgcagctctt gaaagaggtg cgatgaattc
     8461 aggattaccg ccgcttactc ctttattacc tgaaggcatc tgaacagaat gtgtcgcatc
     8521 aaagatcaca ggataaccgg atttcttcat aatgacaaga cttctcatat ccaccactaa
     8581 attattgtat ccaaatgtag tacctctttc tgtgagtaat atttttttgt tgcctgttgc
     8641 ttcaactttt tttgcctgaa agatcatgtc agccggtgcc atgaactgac ctttcttaat
     8701 gttcacaatc tttcctgttt cgcctgctgc ttcgagtagc tcggtctgcc tgcagagaaa
     8761 tgccggtatc tgtaatacat ctgcaacttc agaagccagc tctgcatcga gttcggaatg
     8821 tatgtcagtc aatattggca catcaaattc cttttttgcc attgccagaa tattcagaga
     8881 ctcatcaata ccaagtgtcc tgaaagaaga tgtagaagtt ctgttggctt ttataaaaga
     8941 agatttgaaa ataaagggaa gtttgtattt ttctgaaagc ttttttaatg cttcacatgt
     9001 tttgaatata acctgttttg attctactac acatggacct gatataagca gaaaattctc
     9061 agggtttttt atatttaatt cttttttaaa atatgatggg agcatttaat ttatctgttt
     9121 aaataatctg ttaatttttc ttttaactca gcttttgtat atcctgatct ttcatacatt
     9181 ccttttgcaa gtctttgtct tagtgcttca tatacacaga ctgctgctgc aactgacaca
     9241 ttcagcgact gtattagtcc aaacatcgga attaaaaaat ttttgtccga catttctttt
     9301 acttcatcag aaacacctgt atgttcatta ccaattacga tagcaacttt ttttgtcagg
     9361 tcaagatcat aaagattcag attctttttt ttctcttcat tcatatgagt ggaataaatc
     9421 ctgaacttat ttttcttcag ctcttcaaaa caatctttca cactttcaaa tttttctgta
     9481 gttatccatt ttttagcact tgctgaagaa gtctttccga tctttggaaa tttatttgta
     9541 ttatagatca ggtaaatttt gtcaataccc gctgcttccg cacttctgaa aatggcactc
     9601 acattgtgtt gatcatgtat attctcaagt acaagtgtta aaaacttctg ccgtttactg
     9661 atcacatgac tgaacttatt aaatcttttt ggagtcaaac gtgtttggaa ttttaaatca
     9721 aaaattaatt aattaacttt ctttaaacaa ttaacaaatc atgttattat gaaaaaaata
     9781 tatgttccat taattgcatt atttttgctt acatttattc tcacatctgt ttcatattct
     9841 cagatcaaat ataccaaacc cgaaccacga aatatctggg gctttggatt ttcatattcg
     9901 gaaagaggtt tcggaccgaa tgcaagttta tatgttccaa ttggaaaaac aactgatgta
     9961 ttttttaatg tgctgttttc taatgttacc gattcacgtg agactgaaag gacagatcca
    10021 ttcggaaata cgtttgtaat caataaaata aacagagtat tcatgatgcc gctcagtata
    10081 ggattaagaa aggaattgtt tacagatgat atagaggggg aatttgttcc tatatttaat
    10141 gtaggtattt cgccgacttt aattttcctc aatccatatg acagagattt cttttcagca
    10201 cttggttata ccaccacaca atttgcactg ggaggattta caggactggg tgtaagcttc
    10261 aggcaaagca agaacatgtc attaaatttc aatctaagtt attattatat ctctacatta
    10321 ggagatccgg tccagagtct gaatggaagt gatataaata atgtcggcgg tcttcagctt
    10381 ggatttgcaa ttaattttat gaattgatcc ggataatttt ttttatctgt gttacaataa
    10441 tttacatttc gaaaacatta ctgtaaaata tactttgtat ttttgtaatg aattcattta
    10501 ctaaattata aaaataagga gataagaaat ctcagatcaa attaataatc agccagcagc
    10561 agaggtaaag aaaccttttc taaaaagaac cggagtaagg attacacttt taataatgct
    10621 gatattattt ggagtgatca cagtaaaagg aatagcaatt gcaaaaaaca ttcacaaatt
    10681 cgcagacggt cctgaaggat tcataattga caagatctca gaaaagctcg atctcaatgc
    10741 ttcacaaaaa actcaggtgg aaagaataaa agctcagata aaagaaaaaa tggaatctca
    10801 aaaacctgat cgtgaaagta tgatgaaaga attttctgaa gaatttacaa agagtactct
    10861 cgataaaaac agcatactgg atcaaatgaa aaaaaatgaa agtaagagag aagaaatgaa
    10921 aacttttatg atcgataagc ttgtggagtt ccatgcaata ctaactcctg aacagagagt
    10981 aaaagccgtt gagctgatgt ttgaatttaa agaaatgaaa gataaacatc agccacgagg
    11041 acccggaaat ttcatgaaat aggaaactga aattaatcta atttaatcta aatgaagagg
    11101 gggcattgcc ccttcttttt acattatgaa aaaaattctt ataattgacg atgacaaaaa
    11161 actgtcagat cttcttatag attatctgaa aaaatacagt tttgaacctg tttcagttga
    11221 gaatccttta gacggattat cagaacttga aaaaatcaat tatgattttg taatactcga
    11281 tattatgatg cctgatatgg atggatttga aacgttaaaa gagatacgaa aaaccaaaga
    11341 tattcctgtg ataatgctta ctgcgcgagg tgaaactaca gataagattg tcggactcga
    11401 acttggtgct gacgattatt tatccaaacc atttgagccg cgtgaactgc ttgcaaggat
    11461 tcagtctgta tttcgaagaa cgaaaaataa aagaaacggt aattccattt ttgattttaa
    11521 agaactattc attgaccctt cttcaagaaa aactttgctt cagggaaaga cattagattt
    11581 gacttcaatg gaatttgata ttttattgtt gtttgcagag aataacggga aagtcctttc
    11641 aagagattat ctaatgcaaa aagtaaaagg tactggctgg aattacaatg acagaagcat
    11701 agatgtaatg gtctcaagac tccgcggcaa gcttcataag aaaaaacttg aattcattaa
    11761 gacagttcag tcggtcggtt atatattttt tgctgaagat gaagaagatt aaatttccga
    11821 tattcctgaa attgctgctg ctgtttacag tgttcattat aatagttaat ctgacagccg
    11881 gatttattgc aaagctgagt ctgggagtcg atccggctat tatgataaac aaatatataa
    11941 tgtcattgaa tgaatatatt attaaagaca ttggtaaccc gcctgatatt aaaacagctg
    12001 aagagattgc aaagaaaaaa aatcttgaca tcagaattga aactgacgaa ctgatctggt
    12061 caaattcaga aagaataccg gatctgaagg agctcagaca attccctgaa tttcgtgaag
    12121 gtgctccaag ttttccgatg agattcagag gcggtccgct tttttttaca aagatcaata
    12181 atggatatat tataatctct cctgccaaac atatagaatt catcgaaaaa gaaaaagctg
    12241 tattactaat tatagtagct atatctattc tcacacttgc tttatacttt gctctgcgct
    12301 ggatattcgg accaataaaa tccatttcaa aagacattga agaaatttcc aggggaaatc
    12361 ttaacactag aataattatc aaccgaagag acgagcttgg aagacttgct gaatctatca
    12421 atgagatgaa ggaaaaaatt tccaatatga taaactcaaa agagtcactg ctcattgacg
    12481 tttcacatga actcagaagt ccgcttacga ggatcaaact tgcatgtgaa tttatagatg
    12541 atgaaaagct caggatcaag atcaatgaag atgtgaatga acttgaatct atgatcagtg
    12601 gattactgga cacttacaga aatgaaaata caggtgtcag tttgcaaaag gaaaatattg
    12661 atttaaccgg acttgtgatt aacgtagtca caaaattcaa taatgacaga gttaattata
    12721 taagcgaaat gaacgaatgt tttataaatg cagataagat cggaattgaa actgttttga
    12781 gaaatgtaat tgataactca ctgaaatatt ctgaagataa acctgtgaca gtgaaattat
    12841 cagaacacaa agattcagca aaaattactg taaaggatga aggtaaaggt attgataaaa
    12901 gtgagcttga aaaaatattc gaaccttttt acagaacgga caaatcaaga aataaaaaaa
    12961 ttcagggtta cggattaggt ctcagcctcg tcaaaaaaat actcgacggt catggatttc
    13021 aatttgaaat taaaagtgaa cctggtgctg gctctgagtt tgtgatccat tttcatgtag
    13081 tcagataagt taaaatcaac tttaattctt tttccgcaga gtccgaagag cacgtgactc
    13141 tgcggaaacg gcaactctgt ggaaatggca atcactgaca caccaccttc ctaaaagtca
    13201 tttttctctt tttaacgtac tatagaaaat aaaaaccact attttgataa atataaaaac
    13261 ataggtggaa atttattgat tagtttttta ttttaaccta cagattataa accctcgcaa
    13321 ataaattttc ttaatgtaat ttaaattaat atcatcgtgt taagaaaatg ttagtttttc
    13381 tgaaatcata tcccgcttaa agcagagctg taaatgagaa gttatttaaa aattgcgatt
    13441 ggtattttta tcatctttat ttcatccata gattcatact ctcagtttgt gaattttgga
    13501 aggaataaag ttcagtacgc agattttgaa tggagtgttc tttcaaccga acattttaag
    13561 atctactatt atcaggatgc aaaagagctc gctgagatcg gagctaatta tgctgaagaa
    13621 agttacagag agcatcagga aaaatttaag cactcactcg tcgatacagt accaatgatt
    13681 ttctatacat cccctctgca tttcaaacag acaaatacaa caccgggatt aattcctgat
    13741 ggggtaggag gattttttga atttattaaa ggaagagttg taattccttt tgagggatca
    13801 ctttcacaat tcaggcatgt cattaaacat gaacttgtcc atgtatttat gatgagtaaa
    13861 attggtaatg tgcagaagac tcacaggaaa tctatcggtg ctgcaccgcc actctggttt
    13921 actgaaggac ttgcagaata ctggtccaca caatgggata cacaggctga catggtttta
    13981 aaagatgcag tgcttacagg atatcttccg ggactcagta actgggaaag agtttacggg
    14041 acatatctga tgtataagct tggtcagaaa gtacttgaat acattgcttt gaattttggt
    14101 gaagataaga tacttcagct catcgataat ttctgggtcg atgagaattt ttcaaatgta
    14161 atgcagtata ctcttggggt tgattacgca cagtttgata aaaattttct ttactggctg
    14221 gaaaagcaat attaccctga gtataaggat ctggatgatc cttcagcggt gtcagagaat
    14281 atttacacag aatcttttgc gcataagccg acatattcaa atgctaacgg caatcaggaa
    14341 gtttttttta ttggaaataa gacgggcttt acaagtatct ttaaacttaa tttaagaaag
    14401 ctcagtgatg gagcggatct tgtgatcgga ggagagtcaa gtgatgaatt cgaacagttc
    14461 cattttttca ggacggggat tgatgtttct tctaaaggaa atcttgcatt cattactcaa
    14521 aagggaaatg cagatgcact tcacattcta aacactagga caaatgaagt aatagccgac
    14581 tattcatttc cggatattgt aagtattggt tcaccgtcat ggagtcatga cggaaacaaa
    14641 atcacatttc ctgcaacgga ttttggtggg atcaatgatc tctacattct ggatcttttg
    14701 accaacgaac tcacaagact taccaatgat tattatgatg acagagatcc ggatttttca
    14761 cccgatggag atcatattgt attttcatca gacagaacta cctttggcaa aaagaaagca
    14821 tataatttat ttatatatga aataaaaaca ggcaggatag attatctgac tatcggtgac
    14881 caaattgatt acgcaccgaa tttttcagag gacggtacta agatcatttt tacttctgac
    14941 attaacggac cgcagaatat atggatgata gatctgaaaa aagctattac ttcagaaaaa
    15001 tctgaatccg gaactaatgc tcttgaagac aaaagcggta taaattatga tgatctggaa
    15061 atgagacaga tcacatattt tatttctgca gcttatgatc ctaaatggag cggtaaggac
    15121 agaattgtat tttcttcatt tgaaaaagga tctatcagta taaaaatgtt aaggagcatt
    15181 caggaaaggt ttgacagtag taagaatgtt gtaaaaacta atattaagga gaaaggtgaa
    15241 aactggacag cggataaaat taagagctac acaaataaaa ataatgttcc atataaaaaa
    15301 gatttttctt tagatattgc aactacagct ttgactgtgg atcctgtatt tggagcaaac
    15361 accggcggta ttatttcact aagcgacctg ctgggaaatg aaaaatatta ttttttgata
    15421 tttaataatt caaacggaag tgaagatttc tggaacagtt ttaatattgc aatatcaaaa
    15481 atttctattg agaacagatt aaattatgct tacggaattt atcatctgtc aggaagaaga
    15541 tatgatctgg gagaatttaa ttcgtcgttt tatgaaagac aatatggcgg ttatcttagt
    15601 cttgcatatc cgttatcatt tttcaggaga ctggaaatga ctacaagtct ttcgcaatct
    15661 acaaggagtt acgatctttt tgataatacg cggtcacttc ttctttcaaa ttttatcagc
    15721 tatgttaaag acaatactct ctggaattat accggtccga tggacggcga aagacttaat
    15781 ctcactcttg gatatacgac agatattgca aattcaaaca taaactatta ttcattattt
    15841 tttgatttca gaaaatattt cagattggga ggacctacag ctctggcgtt aaggacacaa
    15901 ttttttatga atgaaggtaa ggaagaaaga agatttttta tgggtggcag ctggtctctc
    15961 agaggatggc cgttaaattc tataagagga agcaaactgt ggcagacaaa tcttgaatta
    16021 agatttccac tgctaaatgt tattgctctg cgtttccctc tcggaatagg atttgatttt
    16081 ccgggtattc agggcgcagt gtattttgat gcaggaaatg catgggatcg aaattataca
    16141 aatgtttata caaacactta cacccgcgga agtataggag ctggtatcag attaaatgca
    16201 ctcgggcttg tcgtgctcag atatgatttt ggaaaaagga ttgaagaaaa ttttacaaaa
    16261 ctgcagggtg atttttatca gaatttttat ttcggatata atttttaatt caattcattt
    16321 ctattttatg caggagggtg attgttcata tttaaaatgt ccattacttt tccagcgatg
    16381 tgatcagcca gatcattaaa atccccggga ttgtattcaa aacttggcat agccggtaca
    16441 atctttccgc ccgcttcaat aacctttatc atattgctga gatggatctt attcaacgga
    16501 gtttctctcg gaaccatcac aagaggtttg gaatatgaca aaccataatc tgccgacttt
    16561 tccataaggg ttttacattc cccgtttgca ataccggcaa gtattcccat tgagcagggg
    16621 gcaatgacca ttccgtgaca attaaattta tttgtaaata tttctgattt gaggtctagg
    16681 ttattatgaa atgaaactgt ggattttaaa atcaggatct ccggaaataa agtctgaatt
    16741 acattcggct tgagatcgat cccgcattct ttgtacattg tgtaagaagc atattccgat
    16801 aaaataagat ctacattaaa atcattgata agaagtgctc ttaaaattct gagtccgtaa
    16861 attgcaccac ttgtacctgt gattgcaaga atgatgttct ttccttctcc gtcatatgtc
    16921 tttattaaag atgatgtttc tgtctgtata actccggaga ttttttcgtc tgccatagtt
    16981 gaatttaatt tcggtttaat aaatatgaaa ctgtgataaa taatactgtc gcagttatta
    17041 caaatataat attttgaaat ataaaaatct gaattaaaaa cactgctaca cctatgacca
    17101 tcgatattag tacttcaaga aaccgtgttg cagaattata ttttgaactc tccagtgtga
    17161 atggtaatgt tttgtcaaat aatgaagcta ttgaaatcac aaaataaact gagcttgata
    17221 tgtaaataaa attaaggaat attgtcagcg tatctgcttt aaataaaagc attaaaaata
    17281 taaaaattag catcggaaga atcatcacaa aataaataaa cgaatttacg cctttaatat
    17341 aagatgaaat atttccggat ggaagagaat caaatatcca ctttgtatct gatgagctgt
    17401 catcgagaat ttttgtattt gaaatcagca tccttgcaca caaaagtaca gtgagcgata
    17461 ccgaaggact gatcactaaa aatgctgttc ttaaaaatcc tgatttgctt tcaaaaaata
    17521 aatattcagc agagtttgaa aaaaatccaa tgatcactac aataacaggg attatcaata
    17581 gcggaatata ttttagtctc agataacggg aattatttat gtgatctctt atcagacgga
    17641 atgaagctaa ttcaatatta ctgccgggaa gaagccttgt aaataattca gatgcttttt
    17701 taaattcagg gaatttgaat tttgatgttt tatttctttt aagagata
//
LOCUS       DAORVQ010000048        17741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1329_length_17741_cov_5.232840, whole genome shotgun sequence.
ACCESSION   DAORVQ010000048 DAORVQ010000000
VERSION     DAORVQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 17741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17741
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_17741_cov_5.232840"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3820
                     /locus_tag="PLX80_05785"
     CDS             <1..3820
                     /locus_tag="PLX80_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HQY20340.1"
                     /translation="VYSNWMPIEKNNNTFIETYKSQFQNTSSNITEEQDRGYLDENGV
                     PLLSQNDMAKNTDDSLQSDAISERILNGLTAGDYLGSSVSGAGDVNGDGYEDVIVGAP
                     LSDYTGANSGRAYIYFGGPVFNSVPDKILSGIAGELLGTSVSSAGDVNGDGYDDVIVS
                     LPGYLSNTGRAYIYYGGADMNTTRDITLTGEATGDYFGNSVAGAGDVNGDGFADVIIG
                     APYKATNTGKAYIYFGGSAMNNVVDVTLIQNIASEFFGFSVSSAGDFNGDGFSDVIVG
                     AYGYSNSAGRTYMYLGGNTVNNVADMTLLGFLIFESFGYSVSSAGDFNGDGYSDIVVG
                     GYGYSNSLGRVYLYFGGTVLNSNPGVIITGQNTSDQFGKSVSGAGDVNGDGFDDIIIG
                     AENFSSGTGKAYLFNGGTSSDNNADKILSAENTGDRFGLFVSGIGDVNGDGNSDFIIG
                     APNNDQNGTSSGRAYVYFNSMSGTDIEDLTFIGTADFDDFGMSVSNAGDVNADGFDDI
                     IIGQGKYNSQQGRAYIYFGGSIMDNVADVTLTGVSAGSLFGHSVSAAGDVNGDGFSDV
                     IIGADRYNSNQGRAYIYYGGSSMNNVADVIFTGAASSEFFGASVSSAGDVNSDGFSDV
                     IIGAYGYNAGTNSGRAYIYFGGSVMNNVADVTLTGVTNEDFFGYAVSGAEDVNGDGYS
                     DVIVGAFGYNAGTDQGRAYIYFGGGSMDNNKDITMTGVSASDYFGISVSKAGDVNGDG
                     YSDVIVGASGYNSNQGRSYIYFGGNSMNSTADVIFTGLEQGDEFGCSVSEAGDVNGDG
                     YHDVIIGASAINGSGEGNTYIFWGGSNMNNVADITMAGENNGDQFGKVVSSAGDVNND
                     GLSDVITNADGNDEGGYFAGKVYLIFSTAPVVNPRLVYVKDVPNDQGGKVNLNWVRSG
                     YDVQNISTITDYVVQKSMPPANGNFAWENTAYIPATNEPFYSYTADTPFDSASNNSGV
                     FYFRITARTSITNQYWRSGILYGSSIDNIAPLMVSPFTASQIISNVSLNWNRSTATDL
                     LNYIIYRSTSPTIDPETEPVFASTIDSTYIDTSPLSGNYYYFIVAQDIHNNKSPVAVV
                     ESPNMTLNLTMFIEGFYNTGSNSQVSDSLTVELRNAASPFAVADISKVVVSSDGTATL
                     KFSAAANGNYYTVIKHRNSIETWSNTAIALSRIIASNYNFTTSSSQAYGNNMIQIDAT
                     PVRFGIYSGDVNQDGTIDLSDGSLIDNDAFNFANGYLPTDVNGDEITDVADAVFADNN
                     GFNFVGKITP"
     gene            complement(4535..4978)
                     /locus_tag="PLX80_05790"
     CDS             complement(4535..4978)
                     /locus_tag="PLX80_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY20341.1"
                     /translation="MDKIIWIVIVLLAGAFLPLQAGLNNKLGKAIDSPVYASMFSFIV
                     GAVAMVLYVIFTRQTADLKGISQVPYYVWIAGLLGAFYVTVIILAFPKLGPGLTFGLV
                     VAGQMTASILFEHNNILVAQQSPISFMKIFGVVLIVAGVIIIRKF"
     gene            complement(5181..6683)
                     /locus_tag="PLX80_05795"
     CDS             complement(5181..6683)
                     /locus_tag="PLX80_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20342.1"
                     /translation="MNKIFLIVLALGIYCHNAELYSQVSTNYLFSQSQGTYQEITGDT
                     IVASATHTSIDPFQLNDVTYNTNHLPFIFKFNGINYTDFKINSNGFITFGSTPPVQTN
                     YGPISSSESYERVVSAFGQNLIGVFGTTADFVSTTFILTNVANFKGVVAGRHITAATG
                     IKADTYIVSFDTTLKTITLSKPTDDIIVTGLVIQIAAGSIVRSTEGTAPYRVHTIQFK
                     NFRQFLIIGTDDNFNFQIKLYETSGVIKVVYGNMNEITSSGIFGQVGLRGNVNTDFNN
                     RTNSVSLDWAASTAGTANAATSKLSSSIFPVSGQTYIWSPVPVSLNISFIQEAFYNPV
                     SEKLNLTDTVNIYLRNNTSPYNLVDSAEGLLDSSTLTANVIFNNANSGTYYIQVNHRN
                     CIETWSKSGGEIITAGITNYNFTSLQTRAYGSNEIQVDASPVRFAIYSGDVNQDGTID
                     LTDGSLIDNDASNFATGYLPTDVNGDWIVDVADAVFADNNAFNFISKITP"
     gene            7279..7623
                     /locus_tag="PLX80_05800"
     CDS             7279..7623
                     /locus_tag="PLX80_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20343.1"
                     /translation="MLTILVVFYTIVGIMGIYLFTRLFSLEKTNMLVGIIHGSLGLLG
                     IGILIFYISFVEGESPFMSILLFFIAMCIGGGMLAAKLTGKKFPMWLALIHAIVAISG
                     IYLLYEFWVRVM"
     gene            7804..10695
                     /gene="gcvP"
                     /locus_tag="PLX80_05805"
     CDS             7804..10695
                     /gene="gcvP"
                     /locus_tag="PLX80_05805"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQY20344.1"
                     /translation="MVNYQNPDRFVNRHIGPDETEIKEMLDIIGVSSLDELIDQTIPP
                     HIRSEKELDLDEPLSEYDFIKNLKSIASNNKILKSYIGMGYNPSVTPPVIQRNILENP
                     GWYTQYTPYQAEISQGRMEALINFQTMVSDLTAMELANASLLDEGTAAAEAMLMFNHH
                     KTSGKKEANTILVSKDCFPQTIDVIKTRAVPVGIEVRLADITESELTEDVFAIMVQYP
                     DSNGDVKDYKKLFEVAASKDIFIIAAADIMSLTLITPPGEIGADAVVGSTQRFGIPMG
                     YGGPHAAYFATKEKFKRNIPGRLIGISIDADGSKAYRMALQTREQHIKREKATSNICT
                     AQVLLAVMAGMYAVYHGPKLLKKIAERIHALTVLLDHSLKNLGLNQLNKIYFDTLKID
                     LSENKERINDLKNIAESHNINFRYFDDAVGISINELSELNDIQEIVNVFSEFLKVNSI
                     DINNVNIDPEISFPEGIKRSSEYLTHPVFNSYHTETELLRYMKGMENKDLSLTTCMIP
                     LGSCTMKLNATAEMVGLSFPGFSDIHPFAPLDQTKGYAEIIDELTKDLKEITGFHSIS
                     MQPNSGAQGEYAGLMVIRQFHLKNGDHHRNICLIPSSAHGTNPASAVMAGMKVVVVSS
                     DANGNINVEDLKTKAELHKDNLAALMVTYPSTHGIFEETIKDICKIIHDNGGQVYMDG
                     ANMNAQVGLTSPAIIGADVCHLNLHKTFCIPHGGGGPGMGPIGVAEHLAPYLPDHPVI
                     KIGSDESISAVSSAPWGSASILIISYAYIKMMGAKGLTDATKIAILNANYIKAKLSSQ
                     FKTLYSGKDGRVAHELIFDMREFKASAKIEVEDIAKRLMDYNFHAPTVSFPVPGTMMV
                     EPTESENKTEIDRFCDTMTSIREEIREIENGESDIIDNVLKNAPHTSARIADDNWQHT
                     YSRNKAAFPIKFADSYKFWPSVARVDNAYGDRNLVCSCNPISDYAEESV"
     gene            10804..11493
                     /locus_tag="PLX80_05810"
     CDS             10804..11493
                     /locus_tag="PLX80_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HQY20345.1"
                     /translation="MLSKEKFVEEVDSTIKKRSLLDHPFYQKWNEGKLTLNELQEYAK
                     QYYHFVKHFPRFVSCVHSNCDDVKTRQILLENLADEEGYKTGISDHPKLWLNFTESLG
                     LSEDEVKNAEPIREVEDLVDGMYELTRSPEFTLGLSALYAYESQVPEISRTKIDGLKK
                     FYNIDSEKAVEFFKVHEEADVYHSRDEMEIMNNNNKSLEDQKRLINTVDESSMLMWNF
                     LDGVYNNYCKN"
     gene            11573..11986
                     /locus_tag="PLX80_05815"
     CDS             11573..11986
                     /locus_tag="PLX80_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQY20346.1"
                     /translation="MNTLKEILNQYNNIAVLGFSLKQDRASNRIGRYLSMNGFNVFGV
                     NPNLDISESNGIPVHGDLKSLPKEIEVVNIFRRSEYLYDHVKEILKMDPLPKVIWTQL
                     GVIDPEAKKLAEENGIQYIENKCIMVEHNKIFYNT"
     gene            11987..13924
                     /locus_tag="PLX80_05820"
     CDS             11987..13924
                     /locus_tag="PLX80_05820"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQY20347.1"
                     /translation="MISKKTIYILLSFFIPFVVYLITLAPDVTFIDSGELTAAASALG
                     VAHPTGYPMFTVIGKIFTLLPFGSQVYMMNIMSAVISSLGLVIFFNLMTFMLSGFKLN
                     ADDTVTDKKKFSDDVIYNISLSSVLVLAFSRTFWETANAVEVYSLHSFFLILNIFLLL
                     KGCSYYSGNKISKEDSIKGERYLLLFAFVLGLSFTNHLSTIFLSVGCIYLYFSVFGFN
                     KISFRRILILGIPFLIGYSFYIYLIVRAENPVISWGYPHNFENFWRHFTGKQFSIWMF
                     SSFENAGKQFTYFTKNFPLEFFYIPVLIAIPGLFEIFKRSKRLFYFTLLLFGFCVVYA
                     INYDIYDIDSYFLLAFIVTGIWFGFGLMFILSKIEKSKMQISYALILMCIIPLNLNYS
                     TADESKNYFVKDYTNNIFNSADQNSIIFSTQWDFWISSSLYYQLIKNVRPDIIVIDKE
                     LLRKSWYMHYLETHYPDFYNRSRAEFDAYLAELLKFEKNTDRYTQPKNETDRQDLQKI
                     QNTFMNLQNSFVDKNIEDHSFYTTYEIEQATSEKFGKDYKRVPQGVLIRYSKDKEFVK
                     LREPEITFTVSERPDYHYTFLMNAYFSSFLNNANYFMNYAKYDEAEIMINRALEVKPQ
                     QKDALQLLAKLNQLKTLQNQE"
     gene            13931..15421
                     /locus_tag="PLX80_05825"
     CDS             13931..15421
                     /locus_tag="PLX80_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQY20348.1"
                     /translation="MKFLFSVILIITVFYCDQFSFGKNSKVFQINSDSTESEIIQNKI
                     DSSEILIPDSDTSKILTTTLPEIRIGKILLVGNDVTDDDIITREISIKENTILDLKQL
                     EEDIGNLYKLGLFNKIDVLPVPTDSVNKFDLMIMVEEKFYILPIPQGGFRDGQFTKFW
                     GGVNLIWYNFRGRNENLNLNFGIGYEPFVNLSYSVPWIGENSRFFSSGSIGYSKNYNR
                     SLLTLNDTTSNTIPSSEDNYLNYNFSASYKFGKYFSKYFSVSTNLRYNSVESDPYQPG
                     RTISTDGIDRFFTFGTFSRYDTRDSYEYTLDGSYYSLEYEKVGFGKTIDFNRINFEAK
                     NFLPIEMNVGKYITFANRTFGSISFGGTIPSYQKEYFGYNNIIRGYKKIVFEGENKLG
                     IFSEFRIPVINPDFIKGRDLPLVKSISALKNLNYKFGLYATIFYDVGGVWYKDDNFFD
                     TQFHSGYGVGLNFILPFGFVGRTDFSLRQQNNELYTTVLFDLDASF"
     gene            15438..16163
                     /locus_tag="PLX80_05830"
     CDS             15438..16163
                     /locus_tag="PLX80_05830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQY20349.1"
                     /translation="MEYYYTTSPNINETEGRLIIDEYEFRHLVKVLRKKTGDKITVTD
                     GLRNIYNCEIRKIDRDKMHCRILSKDFNLFEPDIDLTLCIAPLRNMSRYEFAMEKAVE
                     LGVNTIVPVITDFTILKNEFSRNKRDRIEKIIIAAMGQSQRCFKPLFEKCVKLNEFIK
                     KFSDEKNKVVMYEYSEDMKPYSYDSSSDRVILLIGPEGGFSKEEIRDLRNSGWQIKSL
                     GSRKVRAETAAIVSINQIISKLN"
     gene            16171..17088
                     /locus_tag="PLX80_05835"
     CDS             16171..17088
                     /locus_tag="PLX80_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20350.1"
                     /translation="MNKLIFTFLFIFLVFFIQKSSVSQFDKPVVQLSFGMSNPYDELR
                     GDSYVSFDTLGFIFIDSSLFANHLGGQIGIQLAGAVKINFDKYSISRGVFSLAFNNFN
                     TFQSRQSGTTLVKFINNTFQQRPVEYNYDFNSFTMGFGLEIAPTSFTNIVSPFFNSNL
                     TLNFLSAELTRVTSFNDSNKVSLSSFRMGLNFNAGIEVKVSSNVGFVAGIKYDLANLI
                     YKETLRDGFIEWGSSSGNINDAQGRYISNIYYPIGEAYNYFESKEKEINWGTAYIGIN
                     INLFSDSPKKKTKKTSGGIRSINFYNNEF"
     gene            17155..>17741
                     /locus_tag="PLX80_05840"
     CDS             17155..>17741
                     /locus_tag="PLX80_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20351.1"
                     /translation="MQNNLYSQKIPSLQISIGITSPGGDLGGEFISVNDSGYYNISED
                     FVKNNYGISTGITVAGSIVFPFDEKNLLNFIFSGEYTNMNSFKRSIFGITKVNGIEVP
                     ITYDNAFSGSMFGLGLSTNPAVGKKLSLYVNSIMTMNFLSLSFTQNDVFKTYFSDSFR
                     MGIKSGAGINYKLTNEYSLLLGGSYHFSNLFFKSV"
BASE COUNT         6016 a         2630 c         3301 g         5794 t
ORIGIN      
        1 agtttattca aactggatgc cgatagaaaa aaataataat acttttattg aaacttataa
       61 atctcaattt caaaatacca gtagtaatat tactgaagag caggatcggg gttatcttga
      121 tgaaaacgga gtacccctgc tttctcaaaa tgatatggca aaaaatacag atgattccct
      181 tcagtcagat gcaataagtg aaagaattct gaacggactt actgcaggag attatttggg
      241 ctcgagtgtt tcaggagcag gtgatgtcaa tggagacgga tatgaagatg taatagttgg
      301 tgcaccattg agtgactata ccggtgcaaa ctcaggcaga gcttacattt attttggagg
      361 tccggtcttt aattctgtac cggataagat tctgagcggg attgcaggag agttgttagg
      421 aacttctgtt tcatctgcag gtgatgtaaa cggtgacgga tatgatgatg tgattgtatc
      481 actacccggc tatttatcaa atacaggcag agcttatatt tactatggag gtgctgatat
      541 gaatactaca agagatataa ctttgacagg tgaagcaacg ggagattatt ttggtaacag
      601 tgttgcggga gcgggtgatg taaacggtga cgggtttgcg gatgtaataa tcggtgctcc
      661 ttataaagca accaatacag gaaaagctta tatttatttt ggaggaagtg ccatgaacaa
      721 tgtagtggat gtgactttaa ttcaaaatat tgcttcagaa ttcttcggat tttcagtttc
      781 atcagccggg gattttaacg gagatggatt cagtgatgta attgtaggag cttatggcta
      841 tagtaattcg gcaggaagaa catacatgta tctcggagga aacacagtta acaatgtagc
      901 tgatatgaca cttctgggat ttttaatttt tgaaagtttt gggtattcag tttcttcggc
      961 aggagatttt aacggtgacg gatatagtga tattgtagta ggaggttatg gatattcaaa
     1021 ttcacttggg agagtatatt tatattttgg cggaacagta ttaaattcaa atccaggtgt
     1081 tattattaca ggtcagaata cttcagatca gtttggaaaa agtgtctccg gtgcaggtga
     1141 tgtgaatggt gacggatttg atgatataat aatcggtgca gaaaattttt catcaggaac
     1201 cggtaaagca tatttattta atggcggaac ttcatcagac aataatgctg ataaaattct
     1261 atccgctgaa aatacaggag atagatttgg cttatttgtc agcggtatcg gtgatgtgaa
     1321 cggtgatggc aattcagatt ttataatcgg cgctccaaac aatgatcaaa acggtacaag
     1381 ctccggcaga gcatatgtat attttaactc aatgtcggga acggatatag aagatctgac
     1441 ttttatcggt actgctgact ttgatgattt tggaatgtca gtttcaaatg caggagatgt
     1501 aaatgcagat ggatttgatg atataattat tggacaaggt aaatataatt cacaacaggg
     1561 aagggcatat atttattttg gaggtagtat tatggacaat gttgccgatg taactttaac
     1621 tggtgtttca gcagggagtt tatttggtca ctcagtttct gctgcaggag atgtaaatgg
     1681 cgatggattt tctgatgtaa ttattggtgc agacagatat aattcaaatc aaggaagggc
     1741 atatatttat tatggcggaa gctcaatgaa taatgttgca gatgtgattt ttacaggtgc
     1801 tgcttcgtct gagttttttg gtgcttcagt gtcttcagca ggagatgtaa acagcgatgg
     1861 attcagtgat gtaattattg gtgcgtacgg atataacgct ggaacaaata gtggaagagc
     1921 ttatatatat tttggtggaa gtgtcatgaa taatgttgct gatgtaactt tgaccggagt
     1981 taccaatgag gatttttttg gctatgctgt ttccggtgca gaagatgtaa atggagatgg
     2041 atatagtgat gtaatagttg gtgcatttgg atataatgca ggaacggatc agggcagagc
     2101 ctatatatat ttcggtggag gttctatgga caataataaa gatataacta tgacaggagt
     2161 tagtgcatca gattattttg gaatttcagt ttccaaagcc ggagatgtaa atggagatgg
     2221 atatagtgat gttattgttg gtgcttcagg gtataattca aatcagggaa gatcatacat
     2281 ttattttggt ggaaattcta tgaatagtac agcagatgta atatttaccg gacttgagca
     2341 aggtgacgaa tttggatgtt cagtatctga agccggagat gtgaacggag atggttatca
     2401 tgatgtaatt attggtgctt cagcaattaa tggttcaggt gaaggtaata cttatatttt
     2461 ttggggagga agtaacatga ataacgtagc ggatataact atggccggtg aaaataacgg
     2521 agatcaattt gggaaagtcg tatcttcagc cggagatgta aataatgatg gtttaagtga
     2581 tgtgattaca aatgctgatg gtaatgatga aggcggatat tttgccggta aagtttattt
     2641 aattttctca actgctcctg tagtcaatcc aagattggta tatgtcaaag atgttccaaa
     2701 tgatcagggc ggaaaagtaa atcttaattg ggtcaggagc ggatatgatg ttcaaaatat
     2761 aagtactatt acagattatg tggtacagaa aagtatgccg cctgcaaacg ggaattttgc
     2821 atgggaaaat actgcatata tccctgcgac caatgaacct ttctattcct atactgcaga
     2881 tacacctttt gattctgctt ctaataactc aggtgttttt tatttcagaa ttactgcaag
     2941 gacatctatt actaatcaat actggcgatc aggaattctg tacggaagta gtattgacaa
     3001 tattgcacca ctgatggtat ctccttttac tgcttcacaa attatatcta atgtcagctt
     3061 aaactggaat cgcagcactg ctacggatct gcttaattat attatctaca ggagtacttc
     3121 tccgaccatt gatccagaga ctgaacctgt atttgcatct actatagatt caacatacat
     3181 tgatacttct ccgctcagcg gaaattatta ttatttcatt gttgcacaag acattcacaa
     3241 taataaaagt cctgttgctg ttgtcgaaag tccgaacatg actttgaatc ttacaatgtt
     3301 tattgaagga ttttataata ccggaagtaa ttcacaggtg agtgattctt taacagttga
     3361 attaagaaat gcagcttcgc cgtttgctgt tgctgatatt tcgaaagtag ttgtatcttc
     3421 tgacggaacg gcaactttaa aattcagcgc tgctgcaaat ggaaattatt atacggtaat
     3481 taaacacaga aactcgattg agacatggag taatactgcg atagcattat caagaattat
     3541 agcgtcgaat tataatttta ctacttcttc atcacaggca tatggaaata atatgataca
     3601 gattgatgcc acacctgttc gttttggaat ctactccggt gatgtaaatc aggacggaac
     3661 aattgatttg tccgacggaa gtctgataga taatgatgca tttaattttg caaatggata
     3721 tttgccgact gatgtgaatg gagatgaaat aacagatgtg gctgatgcag tgtttgcgga
     3781 taataatggg tttaattttg tggggaagat aacgccatag tagcatttaa aaatttcgaa
     3841 ttaaaaatta agaattgatt tattgaaacc tatattaaaa tataaaagaa agaaaatttt
     3901 caacagatgt tgctgagata aatatcaaaa ttttaaaaga aagtttcaga ataaatcaaa
     3961 aacgattaac ccaaaaatgg agcgaagagt gttgttgcca aaagaaacag caaacagcaa
     4021 attgcaaaaa tttaaaacca taaataatgg agtcaaatta aactcatttc tacatcaaca
     4081 agtctcccct ttgaaataag gtaaggagca gtaacagttt aataatgctt tttggttctg
     4141 gctatgccag tgtatgagta tgtagaaaat agttaatagt taataaaaga tatgaaaatt
     4201 tgatatttaa aaatttatat ccggagtttc agttatcaag actccggatt ttttattaaa
     4261 aatctgtaaa tcctatttgt aaattttaat cttttaagtg catgttagaa tgagaaaatt
     4321 tgtgaaaaaa aatatttatt aaaatatatt ccactataag tttatatagc accagaatta
     4381 tattcaaaaa acatttatga aaaaaccttt gagacatgga gatatgtcaa aatttagtat
     4441 tggaaagact ttccagcttg agaatgatat aatgatttga gtaagggatt cctgaaggtg
     4501 aattattatt ctttaaaaaa ggataaacaa aaacttaaaa cttccttata ataattactc
     4561 ctgcaacaat taatacaact ccaaatattt tcataaaact gatcggagat tgctgagcaa
     4621 ccagaatatt attatgttca aataatatag aagctgtcat ctgtccagcg actaccaacc
     4681 caaaagtaag accgggtcct aattttggaa atgcaagaat aattacggtg acataaaatg
     4741 ctcccagtaa tcctgctatc cagacatagt aagggacctg actgatgcct tttaaatcag
     4801 cagtttgtct ggtaaaaatc acataaagca ccattgcaac agcgccgact atgaaagaaa
     4861 acattgaagc atatacagga ctgtcaattg cttttcctaa tttattgttt aatccagcct
     4921 gcaatggaag gaatgctcct gcgagtaata ctattacgat ccatattatt ttatccatat
     4981 ccgatattaa aataaattcg aaagtctgat aatacataaa ctttattgaa taataaataa
     5041 gtaatcttct gcagtaaagt ttttagaaac ataaagctcc cagtttatca tcttaaaaca
     5101 ttttaaaaaa aatcccggtc tattgaccgg gataaaattt aaatttgaat tattttaatc
     5161 aaacattgct gttgcatatt ttatggagtg atcttactga taaaattaaa tgcgttatta
     5221 tcagcaaaca cagcatcagc tacatctact atccagtcgc cgttaacatc tgtcggcaaa
     5281 tatcctgtag caaaatttga tgcatcgtta tctataagac ttccgtcagt cagatcaatt
     5341 gttccgtcct gattcacatc tccactgtaa attgcaaagc ggacaggtga ggcatctacc
     5401 tggatctcat tgcttccata tgcacgtgtc tgtaaactgg taaaattata attggttata
     5461 cccgctgtaa taatttcgcc gcctgattta ctccaggtct cgatacagtt tctgtgattc
     5521 acctgaatgt aataagtacc ggagtttgca ttattgaaaa ttacatttgc tgtcagagtt
     5581 gaagagtcta ataatccttc agcagagtca actaaattat aaggagaagt attatttcga
     5641 agatatatat ttacagtatc agttaaattt aatttttctg aaactggatt gtaaaatgct
     5701 tcctgtataa atgaaatatt taaacttact ggaaccggag accaaatata tgtctgtccg
     5761 gaaacaggaa atatactgct gctaagcttc gatgtggcag catttgcagt tccggctgtt
     5821 gatgcagccc agtccagact aaccgaattt gttctgttat tgaaatcagt atttacattt
     5881 cctcttagtc caacttgtcc gaatatacca cttgatgtaa tttcattcat atttccatag
     5941 accactttaa ttacacctga agtttcgtaa agctttatct gaaagttgaa attgtcatcg
     6001 gtacctatta tcaggaattg tctgaaattt ttaaattgaa tagtatgaac cctgtaagga
     6061 gcggttcctt cagttgatct gacaatactt ccggcagcta tctgaattac cagaccggta
     6121 actataatat cgtccgtagg cttagataat gttattgttt ttaaagtggt atcaaatgag
     6181 acaatataag tatcagcttt gatacctgta gcagctgtta tatgtctgcc cgcaactaca
     6241 cctttaaaat ttgcaacatt tgttagaata aatgtagtgg aaacaaaatc agcagtggtt
     6301 ccaaacacac ctatcagatt ctgtccaaat gcgctgacaa ctctttcata actttctgat
     6361 gaagatatag gtccgtaatt tgtctgtacc ggaggagtag atccgaaagt aataaatccg
     6421 tttgaattta tcttaaaatc tgtataattg attccgttga atttaaaaat aaaaggtaaa
     6481 tgatttgtat tgtaagttac atcattcagc tgaaaaggat caatgcttgt gtgtgttgcg
     6541 cttgctacta ttgtgtctcc tgtaatttcc tggtatgtac cttgtgattg agaaaataaa
     6601 taattcgtac ttacctgtga atataattct gcattgtgac aataaatgcc taatgctaat
     6661 actattaaga atattttgtt cataataatc atttttaaat ttaagaaaat ttttgatttt
     6721 ttgaaaacaa tattctcctt tcataaagcc ggtattcctt acttgtaaag attactattt
     6781 ttagggtaaa taatataagt ccgggaagac cttaagtttg aagttgtata ctgaattgtt
     6841 gcctcctttc ggtgtattgg atttagttag aaatattatt ttaaagaaca ataaagaaat
     6901 ttatttcggc taatttaatt taaagacatt atcattttta taatccgtag atcaggtgaa
     6961 ataaaataag atttgaggct actgagattt aaaattcagg ttagaattat gacgggtctc
     7021 aagagttatt cttctttaag ttttcaccaa atatattgaa ttgatgttcc gttaaaaaat
     7081 ctttgaacaa ataattaact tattatatcg taccttaatc tactgttaat tacaagtgaa
     7141 tactatttac tgcctgataa ttttcccgca agcattaaga gatcatttta ctaaaataca
     7201 tcaatattat aataacagcg aaaatgtttg ttcatataat cttgaaatta ttataattaa
     7261 gtttattcaa aaaaatttat gctcactata ttagtagtat tttatacaat agtcggaatt
     7321 atgggaatat atctttttac aagattattc agtttagaaa aaactaatat gctggtcggc
     7381 atcatccacg gatcactggg tttactcgga ataggaatac tgatctttta tatatctttt
     7441 gtagaagggg agtcaccttt tatgagcatt cttttatttt ttattgcaat gtgtatcggt
     7501 ggcggaatgc ttgcagcgaa gcttaccgga aaaaaatttc cgatgtggct agcgttgatt
     7561 catgcaattg tcgcgatctc agggatttat ttgctttatg agttttgggt gagggtgatg
     7621 taaaaaatat aaggttaaat tagaaagtat ggcatattac cggagattgc ttcgctccgc
     7681 tcgtaaaatc atattttaaa aagtaaaatt ttcaaaaatc ccccgatttt agaatttagt
     7741 ttgcagtaaa taaattttat attacttcct tttacaactc aaaatattat acataatata
     7801 tatatggtaa actatcagaa tccagacaga tttgtgaaca gacatatcgg tcctgatgaa
     7861 acagaaatta aagaaatgct tgatatcata ggcgtttctt ctttagatga attaatagat
     7921 cagactattc cgcctcacat cagaagtgaa aaagagcttg atcttgatga accattaagc
     7981 gaatatgatt tcataaaaaa tctgaagtct attgcatcaa ataataaaat tttaaaatcc
     8041 tatatcggaa tgggttacaa tccttccgta acacctcccg tgattcaaag aaacattctg
     8101 gaaaatccgg gctggtatac acaatacaca ccttatcagg cagaaatttc acagggaaga
     8161 atggaagctc ttattaattt tcagactatg gtatcggatc tgactgctat ggaacttgca
     8221 aatgcctcac tcttagatga aggaactgca gctgctgaag ctatgcttat gtttaatcat
     8281 cataaaactt ccggcaaaaa agaagctaat acaattttag tttcaaaaga ctgttttcct
     8341 cagacaattg atgtcattaa aacgagagct gtacctgtcg gcattgaagt aagacttgct
     8401 gatattactg aatctgaact gaccgaagat gtttttgcaa taatggtaca gtatcctgac
     8461 agcaacggtg atgttaagga ttataaaaaa ttatttgaag tagctgcgtc aaaagatatt
     8521 tttataattg cagctgcaga tattatgagc ctgactctga tcactcctcc gggtgagatc
     8581 ggtgcagatg cagtagtcgg ctctacacaa agatttggaa ttccaatggg atacggcgga
     8641 ccgcatgctg catattttgc tacaaaggaa aaatttaaaa gaaatatacc cggaagactt
     8701 ataggaattt caattgacgc tgacggaagc aaagcataca gaatggcttt gcagacacgt
     8761 gagcagcata ttaaaagaga aaaagctaca agtaatattt gtactgcaca ggtgctgctt
     8821 gctgtcatgg caggtatgta cgcagtttat cacggaccca aacttcttaa aaaaatcgct
     8881 gagagaattc atgcgctgac agttcttctt gatcattctt taaaaaatct gggactgaat
     8941 caattaaata aaatttattt cgatactctg aaaatagatc tgtctgaaaa taaagaaaga
     9001 ataaacgacc tgaaaaatat tgcagagtca cataatatta atttcagata cttcgatgac
     9061 gcagttggaa tttctataaa tgaattatca gaattaaatg acatacagga aatagttaat
     9121 gtattttcag aatttctgaa agtaaattct attgatatta ataatgtcaa tatagatcct
     9181 gaaatttctt ttcctgaagg aataaaaaga agcagtgaat atcttacgca ccctgttttt
     9241 aattcttatc ataccgaaac agaattgctc aggtatatga aaggaatgga gaataaagat
     9301 ctttctctga ctacatgtat gataccactg ggttcatgta ctatgaagct gaatgcaact
     9361 gctgagatgg tcggactttc atttccggga tttagtgata ttcatccatt tgctccgctg
     9421 gatcagacga aaggttatgc agaaattatt gatgaactta cgaaagatct caaagagatc
     9481 acaggttttc attcaatttc catgcagcca aattcaggtg cgcagggaga atatgcagga
     9541 cttatggtca tcagacaatt tcatttaaag aatggtgatc atcacagaaa tatttgtctc
     9601 ataccatctt cagctcacgg cactaatcca gccagcgctg taatggcagg tatgaaagta
     9661 gtagttgtca gcagtgatgc aaatggaaat ataaatgtag aagatctcaa aacaaaagct
     9721 gaattacata aagataatct tgcggcactt atggtaactt atccttccac acatggaatt
     9781 tttgaagaga cgataaaaga tatttgcaaa ataattcatg ataatggcgg acaggtttat
     9841 atggatggag cgaacatgaa tgctcaggtc ggacttacaa gccctgctat tatcggcgca
     9901 gatgtctgtc atcttaatct tcataaaaca ttctgcatcc ctcacggagg cggcggtccg
     9961 ggaatgggac caatcggtgt ggcagaacat ttagctccat atcttcctga tcatcctgtc
    10021 ataaaaattg gcagtgatga atcgatatct gctgtttcgt cagcaccatg gggaagtgcc
    10081 agtattctca ttatctctta tgcatatata aaaatgatgg gagctaaagg actgaccgat
    10141 gcaactaaga tcgcaattct taatgcaaat tatattaagg caaaactttc atcacaattt
    10201 aaaactcttt attcaggtaa agatgggaga gtcgctcacg aacttatttt tgacatgcgt
    10261 gaatttaaag cctcagcgaa aatcgaagtt gaggatattg ctaaacgttt gatggattat
    10321 aattttcatg caccgacagt ttcatttcct gtgcctggta ctatgatggt agagccgact
    10381 gaaagtgaga acaaaactga gatcgacaga ttttgtgata caatgacttc tatcagagaa
    10441 gagatcaggg aaattgaaaa tggtgaatct gatataattg ataatgtctt aaaaaatgcg
    10501 cctcatacat cagcgagaat tgcagatgat aactggcagc atacatattc aagaaataaa
    10561 gcagcgttcc cgataaagtt tgctgacagt tataaattct ggccttcagt tgcaagggtt
    10621 gataatgctt atggtgacag aaatcttgtc tgcagttgta atcctatcag tgattatgct
    10681 gaagagtctg tataaacaac agaataaaaa acttaagtaa tctatttata attgtttata
    10741 tattgcttaa ttttagggca ataattttta aaaacttaaa taaatctaaa taaaaataaa
    10801 aatatgttat caaaagaaaa attcgtagaa gaggtagatt ctactatcaa aaaaagaagt
    10861 cttcttgatc atccttttta tcagaagtgg aatgaaggta aattaacttt aaatgaactt
    10921 caggaatatg ccaaacaata ttatcatttt gtaaagcatt ttccaagatt tgtaagctgc
    10981 gttcattcaa attgtgatga tgtaaaaaca agacagatac ttcttgaaaa cttagcagat
    11041 gaagaaggtt acaaaaccgg tatttcagat catccaaaac tatggcttaa ctttactgaa
    11101 agtcttggac tttcggaaga tgaagtaaaa aatgctgagc cgatcagaga agtcgaagat
    11161 ctggttgatg gaatgtatga acttacacgt tcgcctgaat ttacacttgg gctttctgca
    11221 ctttatgctt atgaatcaca agtccctgaa atttcaagaa caaagatcga tggacttaag
    11281 aaattttata atatcgattc tgaaaaagca gttgaatttt ttaaagttca tgaagaagca
    11341 gacgtgtacc actcaagaga tgaaatggaa attatgaata acaataataa atctcttgaa
    11401 gatcagaaaa gattaataaa tacagttgat gaatcttcaa tgcttatgtg gaatttttta
    11461 gatggagtat ataataatta ctgcaaaaac taaattttaa actgaactta ctaaagggaa
    11521 tgattcctct gggatcgttc cctttattgt ttataaatgt ttataaataa caatgaacac
    11581 tttaaaagaa attttaaatc agtataataa tatagcagtt ctcggttttt ccctaaagca
    11641 ggacagagct agtaacagaa taggacggta tctttctatg aatggattta atgtgtttgg
    11701 tgtaaatcca aatctagata tctctgaaag caacggaatt cctgtacatg gcgatctcaa
    11761 aagtttgccc aaagaaattg aagttgtaaa cattttcaga cgatccgaat atttatatga
    11821 ccatgttaaa gagattttaa aaatggatcc acttcctaaa gtaatatgga ctcagcttgg
    11881 tgtcatcgat ccggaagcta aaaaactcgc tgaagaaaac ggaattcaat atatcgaaaa
    11941 caagtgtatc atggtcgaac ataataaaat tttctacaat acataaatga tcagcaaaaa
    12001 aactatttat attttattgt ccttctttat tccattcgtt gtttatttaa ttacattagc
    12061 acctgatgtt actttcattg attcgggaga acttaccgca gctgcatctg ctttaggcgt
    12121 tgcgcatccg accggttatc cgatgtttac tgtgatcggg aaaatattta cgttattgcc
    12181 ttttggttcc caggtttata tgatgaatat aatgagcgca gtgatctctt cattaggact
    12241 tgttatcttc tttaatctta tgacatttat gctttcagga ttcaagctga acgctgacga
    12301 tactgtcaca gataaaaaga aattttcaga tgacgtaatt tataatattt ctctttcctc
    12361 tgtattagta cttgcattct caagaacttt ctgggaaacg gcaaatgcag ttgaagtata
    12421 ttctcttcat tcattttttc tcattttaaa tatttttctc ctgttaaaag ggtgcagtta
    12481 ttattcagga aataaaattt caaaagaaga cagtataaaa ggggagagat atcttttact
    12541 gttcgcattc gtcctcggtc tgagttttac aaatcatctt tcgacaatat ttttatcagt
    12601 cggatgcata tatctttatt tttctgtctt tggatttaat aaaatttcat tccgcagaat
    12661 acttatacta ggtattccat ttctgatcgg atattcattt tatatttatt taatagtaag
    12721 agccgagaat ccggtgataa gctggggtta cccgcataat tttgaaaact tctggagaca
    12781 ctttacagga aagcaattca gtatctggat gttctcatca tttgaaaatg caggaaagca
    12841 gttcacatat tttacaaaaa acttcccgct tgaatttttc tatataccag tattaattgc
    12901 catacccgga ttattcgaaa tattcaaaag aagtaaaaga ttattctact ttactttatt
    12961 actctttgga ttttgcgtgg tatatgcaat caactatgat atttatgata tagattcata
    13021 ctttcttctt gcatttatag ttaccggtat ctggtttgga tttgggttga tgttcatcct
    13081 tagtaaaatt gaaaaaagta aaatgcagat aagttatgct ttgattctga tgtgtatcat
    13141 acctttgaat ttaaattata gtaccgcaga cgaaagtaaa aattattttg taaaagatta
    13201 tacaaataat atatttaatt cagccgatca aaattctata atattttcta cacagtggga
    13261 cttctggatc tcatcgtcat tgtattatca gcttataaaa aatgtaagac cggacattat
    13321 tgtaatagac aaagagcttt tgagaaagtc atggtatatg cattatctgg agacacatta
    13381 cccggatttt tataacagga gcagggcaga atttgatgca tatcttgctg agcttttaaa
    13441 gtttgaaaag aataccgacc ggtatacaca gccaaaaaat gaaactgaca ggcaggatct
    13501 gcaaaagatt caaaatactt ttatgaatct acagaacagt tttgtagata aaaatattga
    13561 ggatcacagt ttctatacta cttatgaaat agagcaggcg acttctgaaa aattcggaaa
    13621 agattataaa agagtaccgc agggtgtttt gatcaggtat tcaaaagata aagaatttgt
    13681 aaagctcagg gaacctgaga tcactttcac agtttcagag agacctgatt atcactatac
    13741 ttttttaatg aatgcttact tttcttcttt tctaaataat gctaattatt ttatgaacta
    13801 tgcaaaatat gatgaagccg agattatgat aaacagggca ttagaggtca aaccacagca
    13861 aaaagatgca ttgcaactat tggcaaaact taatcagcta aagacacttc agaatcagga
    13921 atagtaaaaa atgaaattct tattttcggt aatattaatt ataaccgtat tttattgtga
    13981 tcaattttct tttggaaaaa atagtaaagt ttttcaaata aattcagact cgaccgaatc
    14041 tgaaataatt caaaataaaa ttgattcttc tgaaatctta attccagatt cagatacttc
    14101 caaaatttta acaactacac ttcctgagat ccggatcggt aaaattctac tagttggaaa
    14161 tgatgtaact gatgatgata ttattacaag ggagatcagt attaaagaaa atacaattct
    14221 cgatttaaaa cagcttgaag aagatatcgg aaatttatac aagctcggat tatttaataa
    14281 aatagatgta ctgcctgttc ctacagattc cgtaaataag tttgatctga tgataatggt
    14341 tgaagaaaaa ttttatattc ttccaatacc gcaaggcggc tttagagatg gacagtttac
    14401 aaagttttgg ggtggagtga atctgatctg gtataacttt aggggcagaa atgaaaatct
    14461 aaatttgaat tttggaatcg gatatgaacc ttttgttaat ctgagttatt cggttccatg
    14521 gatcggggaa aactcacgat ttttttcatc ggggtctatc ggttattcca aaaattataa
    14581 ccgcagcttg ttgactctca atgatacaac ttcaaatacc attccgtctt cagaggataa
    14641 ttatttaaat tataatttta gtgcttcata taaatttgga aaatattttt cgaaatattt
    14701 ttctgtttct actaatttaa gatataatag tgtagagtca gacccatatc agccgggccg
    14761 aacaatttca actgacggaa ttgacagatt ctttacattt ggtactttca gcagatacga
    14821 tactagagac tcttatgaat atactctcga tggttcatat tattctttag aatatgagaa
    14881 agttggtttt ggtaaaacaa ttgattttaa cagaataaat tttgaagcta agaattttct
    14941 gccgatagaa atgaatgtcg gtaaatatat aacatttgca aacagaacat tcggatcaat
    15001 ttcttttggt ggtactatac catcatatca aaaagaatat tttggataca ataatataat
    15061 tagaggatat aaaaaaattg tattcgaggg cgaaaataaa cttggaatat tcagcgaatt
    15121 cagaatccca gttataaatc cggattttat aaaaggaaga gatttgcctt tggtaaaatc
    15181 catatcagct ctcaaaaatt tgaattataa gttcggtctg tatgcaacaa ttttttatga
    15241 tgtaggcgga gtctggtata aagatgataa tttttttgac acacagtttc acagcggata
    15301 tggagtaggt ctgaatttca tacttccgtt tgggtttgtc ggcaggacag attttagtct
    15361 gaggcagcag aacaatgaac tatatacaac tgtacttttt gatcttgatg catcattcta
    15421 attaaaacaa ttaaattatg gaatattatt ataccacttc tccaaatata aatgaaacag
    15481 aaggcagact tattattgat gaatatgaat tcagacatct tgtaaaagta ttacgcaaaa
    15541 agaccggtga taaaattact gttacagacg gattaagaaa tatttataac tgtgagatta
    15601 gaaaaattga cagggataaa atgcattgca gaattttaag caaagatttt aatttgtttg
    15661 agcctgatat agaccttacg ctttgtatag ccccacttag aaatatgtcc cgctatgaat
    15721 ttgcaatgga gaaagcagtg gaactaggag ttaatactat tgttcctgtc ataactgatt
    15781 ttacaattct gaaaaatgag ttcagcagaa ataaaagaga caggattgaa aaaataatta
    15841 ttgcagcaat gggacaatcc caaagatgct ttaaaccatt attcgaaaaa tgtgtcaaac
    15901 taaatgaatt tataaaaaag ttttcagatg aaaaaaacaa agttgtaatg tatgaatatt
    15961 cagaagatat gaaaccatat agttatgaca gttcttcaga cagagttata cttttaattg
    16021 gtcctgaagg tggatttagt aaggaagaga taagagatct tagaaattca ggctggcaaa
    16081 taaaatctct tggaagcagg aaagttcgtg cagaaactgc agcaattgtt tctataaatc
    16141 aaatcatttc aaaattaaac tgaaagagaa atgaataaat taatatttac ttttcttttt
    16201 atatttttag ttttttttat tcaaaaatct tcagtttctc aatttgataa acccgtagtt
    16261 cagctaagtt ttggtatgtc taatccttat gatgagctga gaggtgacag ctatgtttca
    16321 tttgatacac ttggctttat atttatagat tccagtttat tcgcaaatca tcttggtggt
    16381 cagataggta tacagttagc aggtgcagta aaaattaatt ttgataaata ttccatcagc
    16441 aggggagtgt tctctttggc tttcaataat tttaatacat ttcaatccag acaatccggg
    16501 actactttag taaaatttat aaataatact tttcagcaac gtccggttga atacaattat
    16561 gacttcaata gttttactat gggatttgga ctcgaaatag caccgacttc atttactaac
    16621 attgtctctc ctttttttaa ttctaactta actttgaatt ttctatctgc cgaacttacc
    16681 cgggtcacca gctttaatga ttctaacaaa gtaagtctca gtagttttag aatgggtctg
    16741 aatttcaatg caggtatcga agtaaaggtc agcagcaatg ttggctttgt agccggtata
    16801 aaatatgatc ttgcaaatct gatttataaa gaaactctta gagacgggtt tatagaatgg
    16861 ggttcgtcaa gcggaaacat aaatgatgca cagggcagat atatttcgaa tatttattat
    16921 ccaattggcg aggcatataa ttattttgaa tctaaagaaa aagaaataaa ctggggtacg
    16981 gcatatattg gtataaatat aaatttattt tcagacagtc cgaaaaagaa aactaaaaaa
    17041 acttcaggtg gaataaggtc aattaatttt tacaataatg aattttaaaa aatttctaac
    17101 gatttgagaa tatttcttct taaagtattt actgtattaa tcatttcatt ttttttgcag
    17161 aataatttgt actctcaaaa gataccttca cttcaaatta gtattggtat tacctcaccc
    17221 ggtggtgatc tcggaggtga atttatatct gtaaatgatt caggatatta taatatcagt
    17281 gaggattttg ttaaaaataa ttatgggatt tccacaggga ttactgttgc aggaagcatt
    17341 gtttttccat ttgatgaaaa aaatttattg aattttatat tttcaggtga atatacaaat
    17401 atgaattcat ttaaacgaag tatatttgga attacaaaag ttaacggaat tgaagttcca
    17461 ataacttatg ataatgcttt ttcgggttca atgtttggtc ttggtctaag tacaaatccg
    17521 gcagttggaa aaaaattatc tttatatgtt aattctataa tgacgatgaa ttttctaagt
    17581 ttatctttta cacagaatga tgttttcaaa acttactttt ctgattcttt cagaatgggt
    17641 ataaaatcag gtgcaggaat aaattataaa ttaactaatg aatattcttt attacttgga
    17701 ggaagttacc actttagtaa tttatttttt aaaagtgtac a
//
LOCUS       DAORVQ010000049        17152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1411_length_17152_cov_5.333684, whole genome shotgun sequence.
ACCESSION   DAORVQ010000049 DAORVQ010000000
VERSION     DAORVQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_17152_cov_5.333684"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLX80_05845"
     CDS             <1..327
                     /locus_tag="PLX80_05845"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20352.1"
                     /translation="LHVSTKWAFENLKFPIDKIRQKKFDNIKVFENKILTELDNDFEP
                     IVFSKFPLLKEVKNFMLNAGAVFSSMSGTGATIYGIFDTNKNGTIYKCREEFIKKKYL
                     TFISKA"
     gene            complement(333..1322)
                     /locus_tag="PLX80_05850"
     CDS             complement(333..1322)
                     /locus_tag="PLX80_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20353.1"
                     /translation="MKKIKEFISVLILLVFTGLTYSQSSELSIDKEIELKKEFPAELH
                     ADLNPDHADNNFLKSELTDKLLNQDSKNYSDLKNFKNFERNLNLESATKSDEKSPYLG
                     ALFSGVIPGSGEFYAKSYIKAAIFFAIEIGLWTAYGVYEGNGNEQTDYYKGVADKNWS
                     VNKYAQWLVDQQFTGFGSITDPQSQDLNTLRRQINIVEAQNFSHQLPPYGDQQYYELI
                     GKYQNFVVGWADADPNISKNSASPNYYGTYKTEMFNTYAVDRQTANDYYNKANTSLAL
                     VLLNHLVSAADAAWSVTMFNKDLKVNTNMKFKNMYGYYGESKLVPFANLNVTF"
     gene            complement(1330..2064)
                     /gene="truA"
                     /locus_tag="PLX80_05855"
     CDS             complement(1330..2064)
                     /gene="truA"
                     /locus_tag="PLX80_05855"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQY20354.1"
                     /translation="MFNYRLTIEYDGRNFKGWQKQKITKETIQQYLEDAIEIILKEKV
                     ILYGAGRTDAGVSAFQQTANFKSEKEFDLKKFLYSLNSILPLSITVKNIRKVKADFHS
                     RYSAKKREYLYFISKDKKSIGGEFYYLINYEPDYKKVDEFIKFIKELEYFKSFCKNRE
                     DKHNFASIIYDFDYKIKNKSKEIVFRISAKRFLHSMVRSILGCTMEIASSKKDIEETK
                     LSILKGDKIRIHYLPSNALFLNKILY"
     gene            complement(2072..2827)
                     /locus_tag="PLX80_05860"
     CDS             complement(2072..2827)
                     /locus_tag="PLX80_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20355.1"
                     /translation="MRKKLFSILIVTALSCNYSYSQDKESNFNNFSNLDLKEKMLTSE
                     TAENKPEINTSITASKKSPAIALLLSLVLPGSGHYYLDRMDVGKYFLGADAVGWIGLV
                     TMNVYGNSVQDDARSYSAIHADITNPGSQDDDFYTNIGSYNSVYEYNNAKLSTGEYNS
                     LYSTSANYWDWDNIDNRNFYNSQRKQSERIYNNRIVFSSLLIVNRIVAGISAYLIGTN
                     IGKKSTSMNISPELLYKKDLSIDGFLLSLNKNF"
     gene            complement(2845..4674)
                     /locus_tag="PLX80_05865"
     CDS             complement(2845..4674)
                     /locus_tag="PLX80_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQY20356.1"
                     /translation="MLYKKAFFFIYILSVLTFGQKLYSQKSDYLEFSVSADREFFSDK
                     DTIRLALNIKIINPYHINSYKVEDPSLINTTVSVNDDNFNFINSFFPESKRLKFEFSE
                     NEIDVYEGIITVGVALTAKENIAEGSYKLPVELSFQACDDRVCYSPKTLTDTIIINLN
                     NNKRSESVQNTELFSKIDFNKPTDENSNNQSGSEKLKENERTDSPVSQEDEVSNWIEE
                     KGLFVSLILIFLGGLALNLTPCVYPLIPITVSFFGAQSSGSKSQSILMGVFYALGMSI
                     TYSALGVFAALTGSLLGTALQNPIVIIFIALIMLALGLSMFGLFEIKVPQGLAQMGNK
                     NRSGYLGSVLMGLTVGFIAAPCIGPFVLSLLVFVGKTGNPFTGFLLFFILSMGLGLPY
                     IFLAASSSSISKLPRSGEWMEGIKVIFGFILFGMAFNTLEPLIPKEIFKVTFPLYIIL
                     SGVYLILFDNKGKTSKVYTNIKYFIAILAVIYGTWNLKPEGAVEEVKWQLHTSLQSIE
                     SSINSGNKPVMIDFYADWCAQCKELDEYTYTDPEVAELSKNFNNIKVDLTKENTEISD
                     KFDIKGLPVVIFMNADGEEIKELRVTGFENPEEFKKKINSILK"
     gene            4934..5182
                     /locus_tag="PLX80_05870"
     CDS             4934..5182
                     /locus_tag="PLX80_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20357.1"
                     /translation="MKPAVKSKSDNPLSELIDDNVYNTLKKYKLLDEKAIRDYKIRQM
                     YKEMRREMSAGEAIDKIQELFPYLQFDTIRKIVYQVSK"
     gene            5250..6068
                     /locus_tag="PLX80_05875"
     CDS             5250..6068
                     /locus_tag="PLX80_05875"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQY20358.1"
                     /translation="MNLNYDHTEKILTGFLKNETHRIGIKKAVIGLSGGIDSAVSAFL
                     SVKALGNKNVTCILMPYKTSSKDSLTDASNVAKILNVESKVIDISETVDTLIKNIGDK
                     KISNVRKGNIMARVRMIILYDESAASNSLVIGTSNKTEILLGYSTIFGDSASAINPIG
                     DLYKTHIRGLARHLNVPENIIIKKPSADLWKGQTDENELGFSYEEIDKYLFDKVDERR
                     SAEYLENAGYEDNFMKRINSLIIRSQFKRLPPLIAKVSNRTVNIDFRYNRDWNT"
     gene            6078..7748
                     /gene="ggt"
                     /locus_tag="PLX80_05880"
     CDS             6078..7748
                     /gene="ggt"
                     /locus_tag="PLX80_05880"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQY20359.1"
                     /translation="MFIKNLLFIFSFLFLSCSSYSNDKGGMVSSASDLATQVGIEIMK
                     NGGNAVDAAVGVGFTLAVVYPQAGNLGGGGFMIIHLNDNGKLLNTSIDYREKAPLAAY
                     KDMYLDKDGNVIDDLSTTGSLAAGVPGSVAGMLYALDKYGTKSRSEILSYAISIADTG
                     FFIEEGLAEALNSYQSEFSQFAGTMNIFGGKFITGERLIQKDLANTLKAVSQNGRDGF
                     YKGEVAEKIVQLMINSNGMISYEDLEKYQPAERDVVKGTYRGYDILSMGPPSSGGISL
                     IYLLNILENYDLAKTGYGSSENIQLMTEAMRRVYSDRSEFMGDADFVNVPIDVMTSKE
                     YAKDRMKDFVTDQAGRSKVIKAGDPFGNESKETTHYSVADGNGNLVSVTTTINDVFGN
                     KQVVVGAGFFLNNEMDDFVSKPGVPNIYGLVGNDANAIQPEKRMLSSMTPTIVFKNDK
                     PYLVVGSPGGGRIITSVLQTIINLIDHNMSLEDAIDSPRFHHQWLPDEVQIENGMLNN
                     EVKNFLQSIGYTFQSISDFGRVNAILISEDGTMTGHSDRRGYGSSVGF"
     gene            complement(7904..8863)
                     /locus_tag="PLX80_05885"
     CDS             complement(7904..8863)
                     /locus_tag="PLX80_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20360.1"
                     /translation="MKFDSDIEKFNNSGSSKPGEFEMWYFDAIDNDDDYYFTARIYYG
                     NPEKVSSNGNPNSDNTNNYCRFRFFLYYQNRLIYDAIYDYKKEEFRIEESDGLVKMIL
                     DKNSFCHDKENDKYLLNINYSSAELESKFKAEFEFKPLLKKSSINKKTDHNLEKEVCW
                     MPSPLCHVSGKIKVYRDYKRVKTIFDGKGYIDKTWGSTNSFNKISYSNWGKFIRDDYS
                     FIFFVSESKGENKFMKLQIFKDNLLLLEKEDFKLLTKKTFFTNKIKSIEIESNDLKVI
                     LKNEINIESGEFGDKYISKFEMYHDEKKVIDNVYGFFQNVVNN"
     gene            9140..10756
                     /locus_tag="PLX80_05890"
     CDS             9140..10756
                     /locus_tag="PLX80_05890"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQY20361.1"
                     /translation="MHQLKILTILFFFISVNSNAQIDTNINVIKYDLEFDLINTFQEP
                     YPKTYSAVNVVSLMVNSTAGQITLNAENYSLIIDSVSGSGQTFVHENDLLKINLDKFY
                     NSGDMFDVKIYFRHKEIFDTAYFAFKGLMYTDSETAGARRWFPCKDIPNDKVMLRLSA
                     RVPEKVLFVSNGLLVDSVYKNNSLHYTWETNIPISTYLIAMAGSKNYKLEVFNWKKLK
                     PPYDNVEIRLYSQPGETKFNYDNIKNKLPEMLEVFSKLYGDYPFEKIAFVTTDRNFPW
                     GGMENQTLVTLCPDCWYESLIVHELVHQWFGDMITPNTWADIWLNEGFATFNEAIWDE
                     YNKGPKEYKKNIQNEAMKYLSGDPGWELYNKDWAVSKPNDDVVFNSNVTYSKAASIVY
                     MLRYVLGDSVFFDALQAYTDNPQFRFGNVTTSEFQAAVEQVSALDLDWFFSQWLMKPG
                     FPEYEFNFTTEKSKNGKWKTDYTINQIQSNKIFYQMPVELKVKFTDGSEQLLKERNSY
                     NFQTFNFEFDKEPERIIFDPNNWIVLKKVK"
     gene            complement(10758..12074)
                     /locus_tag="PLX80_05895"
     CDS             complement(10758..12074)
                     /locus_tag="PLX80_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQY20362.1"
                     /translation="MDLFGNVSDKETEYNAPLAERMRPDSIENFAGQEHLISAGKPVR
                     TMIEKNEPSSMIFWGPPGVGKTTLALLIARSVNSEFIQLSAISSGVKDVRAAIEKAEF
                     NLRQKKRKTILFIDEIHRFSKSQQDSLLQSSEKGTVILIGATTENPSFEVISPLLSRS
                     RVFILEELSKNDFLKIINNALTNDIVLKEYDIKIEDKDFLIGLSSGDGRKLLNGLELA
                     VKLTSKNDEGVIELTNEIFKEAFQANYIKYDKNQEEHYNIISAFIKSVRGSDPDAALY
                     WMARMLKGGEDPKFIARRMIVLASEDIGNADPEALVIAVSAFTAVDFIGMPEATLVLS
                     QAAAYLASAPKSNACYLAISKAKEDVENFPAFGVPMHLRNAPTKLMKDLNYGSGYKYS
                     HDYENNFSEQDYLPEELKEKIYYLPSENGKEAEFKKRLTALWKKRK"
     gene            complement(12080..12994)
                     /locus_tag="PLX80_05900"
     CDS             complement(12080..12994)
                     /locus_tag="PLX80_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY20363.1"
                     /translation="MISRRFSKSHDFLAWSLLIILSVIWGSSYILIKKGLLVYTPLQV
                     GTMRITFGFLVLLPAAVLKIFRLPGRKLFVLLIIGIIGNILPYILFAYSQTVLPSSVT
                     GILNALTPIFTMLIAVFIFKYKIKNMQIIGMLLGFLGVMSLAFVDESGVFGSFNIYVM
                     LVVLATLCYSISLNLIKVYLSDINSVIVTGVSLLTVGPVCLIYLLSTDLIHVFNTVPG
                     SGLAMFYIAILGIVGTAFAMILYTKLIQITDALFATTVTYLLPIVSIIWGILDGEVLY
                     HIHFVIMLIILIGVYLVNKNFKKKEKLI"
     gene            13351..14535
                     /gene="dinB"
                     /locus_tag="PLX80_05905"
     CDS             13351..14535
                     /gene="dinB"
                     /locus_tag="PLX80_05905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQY20364.1"
                     /translation="MSGLKHKIIFHIDMDAFFASCEESINPSLRSKALVVGGTKDDKR
                     SIVACPNYLARAKGVRTAMPLSRAILLVPDGNFIRSTRGLYSDYSKRVREIFFKYTPA
                     VQPVSVDEAYLDVTGVLHLHKNDHILLAERIKDDIKETLDITCSIGIAVNKICAKIAS
                     KINKPDGVTVVPFGNEKEFLSKMTIDKIPGVGKATQERLRKYGINLIGDILKFDQTFY
                     DKEIGFHTAFLINVANGRGSDNVKENSEERKSLSKENTFYEDVSDREFLQSELYSLME
                     RCCQKLRKVKLKGRTVTVKVKYFDFTVNQRSYTTSKYSNLETDFYEDAVSLLDKMLMN
                     KKMVRLLGVKFSEFVAGDDAVQENLFDDVDKKENLLNKLDTIRKKYDFEIVKFGRTMK
                     QK"
     gene            complement(14601..16289)
                     /locus_tag="PLX80_05910"
     CDS             complement(14601..16289)
                     /locus_tag="PLX80_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY20365.1"
                     /translation="MKKFSLYIFFFLSLFLYSCNEKTKTESNVFNINLNQNIETLEPV
                     MSNSIQSIWGLSVMMEGLVQFDKESNILPSIASRWEISNDALTYTFHLKDDVYFHDNE
                     CFKESGGKGRKVTAQDFKYCIERVNNPKTKSRGQWVYRDRIKGAQEYIDFMSGTADKK
                     VDGISGLKAPDDSTLIIELTKPFSPFLSILTMTYGYVYPKEAVEFYGDRFGQNPVGTG
                     PFKFASWNLDQDLNFVKNETYREKDKEGKSIPYLDGIKFTFTQSSETEFLDFQNGKYD
                     YHDPSSETYDQITDENGNLIDSQRDKYKLNRQPWLQTVFFGMMQSPDLPGGKEGPFTN
                     NLKLRKALNYAVDRDRILKFVLKNRGTSAENGPIPPGMPGFDASIKGYKFDKDKAMLL
                     LSEAGYPGGKGLDLTLVTGNEEIQKTLAIAVQQQLKEIGIDLKLDQMIQSTLISKQED
                     GVFPFWRASWGADYYDPENFMALFYSKNITPNGPNRVGYSNPEVDKLYEDALKITDFA
                     ERKKIYDKMQKIVIDDAAWLYLYYNQKIYLLANNVEGFYVDGLNIINIKYTKKN"
     gene            16386..>17152
                     /locus_tag="PLX80_05915"
     CDS             16386..>17152
                     /locus_tag="PLX80_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQY20366.1"
                     /translation="MDKITHDNILKAHCFNELSATDYLVSYRNIGEILKERLSEKPDK
                     IYITFYDDNGKMTSLTYRDFVQRAYQLADFLLKEGVTKGSRVATFSHNHFKTVILYFA
                     SWTIGAVVVPVNVSEESDRVDYILKNSESKIVFTREEYISKLNNSDAVKNIKVIPLDS
                     FSFDLYSDEPDPADLNFDKEAECLIVYTSGTTGNPKGVVLTQYNLMADAFAISEWHGL
                     HNDDVMMCVLPIHHVNGTVVTVMTTMFYGGKLILNQK"
BASE COUNT         5760 a         2851 c         2824 g         5717 t
ORIGIN      
        1 cttcatgttt ctaccaaatg ggcttttgag aatcttaaat ttcctattga taaaatcaga
       61 caaaaaaagt ttgataatat caaagtattt gagaataaaa ttttgactga attagataat
      121 gattttgaac caatagtatt ttcaaaattt cctttactaa aagaagttaa aaatttcatg
      181 ttaaatgcag gagctgtatt ttcttctatg agcggaaccg gagcgactat ttatggaatt
      241 tttgatacaa ataaaaacgg caccatatat aagtgccgag aagaatttat caaaaaaaaa
      301 tatctgactt ttatttctaa agcttaaaga aattaaaaag ttacatttaa atttgcaaat
      361 ggaactaact ttgactcacc ataatatcca tacatattct taaacttcat attagtattt
      421 acttttaaat ccttattaaa cattgtaaca ctccatgctg catcggctgc acttacaaga
      481 tggttcagta atacaagtgc aagtgatgtg ttagccttgt tataataatc atttgctgtc
      541 tgcctgtcga cagcgtaagt attaaacatt tctgttttgt atgttccata ataattcgga
      601 cttgcagaat ttttacttat gtttggatct gcatctgccc atccgactac aaaattctga
      661 tatttaccaa tcaattcata atactgttgg tcaccgtaag gaggaagctg atgagaaaaa
      721 ttctgtgctt ctactatatt tatctgtctt cttaaagtat tcagatcctg tgattgtgga
      781 tctgtaattg atccaaaccc tgtgaattgc tgatctacaa gccattgtgc atatttattt
      841 acactccagt ttttatctgc tactccttta taataatctg tctgttcatt tccatttcct
      901 tcatagactc cataagctgt ccataatcca atttctattg caaaaaatat agcagctttg
      961 atataactct tggcataaaa ttctccagaa cccggaatta caccggagaa aagtgctcca
     1021 aggtaaggtg acttttcatc tgatttagtc gcactttcaa gattaagatt tctctcaaaa
     1081 tttttaaagt tctttaaatc agaataattt ttcgaatcct gatttaataa tttatctgtt
     1141 aattctgact taagaaaatt attatctgca tgatccggat tcagatctgc atgaagttct
     1201 gcaggaaatt cttttttaag ctcaatttcc ttgtcaatgc ttaattcact gctttgagaa
     1261 tatgtcaatc cggtaaaaac cagtaatatt aataccgata taaattcttt tatctttttc
     1321 atttaaagtt tagtaaagga ttttgtttaa aaataatgca ttagatggta aataatgaat
     1381 ccttatttta tctcctttta agattgataa tttagtttct tcaatatcct tttttgatga
     1441 tgctatttcc atagtacaac ctaatattga tcttaccata gaatgtaaaa atctctttgc
     1501 tgaaattctg aaaacaattt ccttactctt gttctttatt ttataatcaa agtcataaat
     1561 tatacttgca aagttatgct tatcttccct gtttttacaa aatgacttaa agtattcaag
     1621 ctctttaata aatttaataa actcatcgac ttttttgtaa tcgggttcat aatttataag
     1681 ataataaaac tctccaccta tagatttttt atcttttgat ataaaataaa gatattctct
     1741 tttctttgct gaatatctgg aatgaaaatc agcttttact tttctgatat ttttcacagt
     1801 tatacttaga gggagaatag agttaagtga atataaaaat ttttttaaat caaattcttt
     1861 ctctgactta aaattagctg tctgttgaaa agcactgaca cctgcatcag tccgacctgc
     1921 tccatacaaa ataacttttt cctttaaaat tatttctatt gcatcttcta aatattgctg
     1981 tattgtttct ttagttatct tttgtttctg ccagcctttg aaattccttc catcatactc
     2041 gattgtaagt ctgtaattga acaaaacttt tttaaaaatt cttatttaaa ctcaataaaa
     2101 atccgtcaat cgacaggtct ttcttataaa gtaattcagg actgatattc atcgaagttg
     2161 actttttacc gatgttggta cctataagat aagcactgat tccggctaca atcctgttta
     2221 caattaaaag acttgagaag acaattctgt tattataaat acgttcactt tgttttctct
     2281 gtgaattata aaaatttctg ttatctatat tatcccagtc ccagtagttt gctgatgttg
     2341 aatacaatga attatactcg cctgtagata acttagcgtt attgtattca taaacactgt
     2401 tataacttcc aatatttgta taaaaatcat catcctgact acccggattg gtaatgtcag
     2461 catgtattgc ggaataagat ctggcatcat cctgaaccga atttccataa acattcatcg
     2521 ttactaaccc aatccagcct acagcatctg caccgaggaa atatttccca acgtccattc
     2581 tatccagata ataatgtccc gagcccggca atactaatga aagtaataaa gcaattgcag
     2641 gagatttttt tgaagctgtt atactggtat ttatttcggg tttattttct gctgtttcag
     2701 aagtcagcat tttttcctta agatctaaat tactgaaatt attgaaattg ctttctttat
     2761 cttgtgagta ggaatagttg caggaaagtg ctgtgactat aagtattgaa aaaagttttt
     2821 ttcgcattaa aattctattg gtttttattt taaaattgaa ttaatttttt tcttaaattc
     2881 ttcaggattt tcaaatcctg tgactctgag ttccttaatt tcttctccgt ctgcgttcat
     2941 gaatattact acgggaagtc cttttatatc aaatttatct gagatctctg tgttttcttt
     3001 tgtcagatca actttaatat tattgaaatt ttttgaaagc tctgcaactt caggatcggt
     3061 ataggtatat tcatcaagct ctttacattg tgcacaccag tctgcataaa aatcaatcat
     3121 cacaggtttg tttcctgaat taatactgct ttcaattgac tgaagagatg tatgcaactg
     3181 ccattttact tcttcgacag caccttcagg tttcaagttc caggttccgt aaatcacagc
     3241 caatatcgca ataaaatatt taatatttgt atatacttta gaagtctttc ctttattatc
     3301 aaaaagtatt agatatactc cggaaagaat tatgtacaac gggaatgtaa ctttaaaaat
     3361 ttcctttgga ataagtggtt caagagtatt aaacgccatt ccaaaaagta tgaatccgaa
     3421 gatcactttt ataccttcca tccattcacc tgatcttgga agcttgctaa tagaactgga
     3481 agaagcggcg agaaaaatat acggtaatcc taatcccatt gataaaataa agaatagcaa
     3541 aaacccagta aaggggttac cggtctttcc cacaaacaca agaagactta aaacaaacgg
     3601 tccgatacac ggagctgcaa tgaaacctac agtaagtccc attaataccg atccgagata
     3661 tcctgaacgg tttttatttc ccatctgagc aagcccctgt ggtactttga tctcaaacaa
     3721 tccaaacatg ctcagtccta aagccagcat aatcagggca ataaaaataa ttacgattgg
     3781 attttgtaat gctgttccaa gcagacttcc ggtcagagct gcaaatactc cgagcgctga
     3841 ataagtaatt gacattccga gtgcataaaa tactcccata agaatacttt gcgatttact
     3901 cccgcttgac tgcgcaccga aaaagctgac tgttatcgga attaacggat atacacaagg
     3961 tgtaagattg agagctaatc cgcctaaaaa gatcagaatc aacgaaacaa ataaaccttt
     4021 ctcttctatc cagtttgaaa cttcatcttc ctgtgataca ggtgaatctg tcctttcatt
     4081 ttcttttaat ttttctgaac ctgattgatt atttgaattt tcatctgtcg gcttgttaaa
     4141 atctatcttt gaaaataatt ctgtattttg tacgctttct gatcttttgt tattgtttaa
     4201 attaataatg atagtatcag tcagtgtttt aggagagtag caaactctgt catcacatgc
     4261 ctgaaatgaa agctccaccg gtaatttata actaccttca gctatatttt ctttagcagt
     4321 taaggctacg ccgactgtta taatcccttc atatacatca atctcattct ctgaaaattc
     4381 aaactttaac ctctttgatt ctgggaaaaa tgaattaatg aaattgaaat tatcgtcatt
     4441 gacagagact gttgtattga tcaatgatgg gtcttctacc ttataagaat ttatatgata
     4501 aggattaata atttttatat ttaacgcaag tcttatagtg tctttatcag aaaaaaattc
     4561 cctgtctgct gagactgaaa attccagata atctgatttc tgtgaatata atttttgacc
     4621 gaaagttaaa acagacagga tatatataaa gaaaaatgct tttttgtata acatcaatta
     4681 atttaattct actgaaagga acttcttttg tatgtataaa agtttaaaat tatcatacaa
     4741 tatactataa aaaataaaaa caagatatta aaattaaggt ctttaaatat tttttaaatg
     4801 caatattgac tttactattg tttttactgt atatttgtat aggaaaattt catactaaat
     4861 tcgatttatt aaagaaatcc gaattttaat gaaaaaactt taaaaactta acttagataa
     4921 ggagaattac aatttgaaac cagcagtaaa atcaaaaagc gacaaccctt taagtgagct
     4981 tatagatgat aatgtataca ataccttgaa aaagtataaa ctccttgatg aaaaagctat
     5041 aagagattac aaaataaggc aaatgtataa agaaatgcgc agagaaatga gcgctggtga
     5101 agcaatagat aaaatccagg aattatttcc atatttacag tttgatacaa tcagaaagat
     5161 cgtttaccag gtatcaaaat aaattagttt tttttttaaa acagccggcc ataatatttg
     5221 gtcggctttt tttttgaatt ttaaaaatca tgaacttaaa ttacgatcat acagaaaaaa
     5281 ttctaaccgg ttttttaaaa aatgaaactc acagaattgg gatcaaaaaa gctgttatag
     5341 gtttatcagg tggtattgat tctgcagtat ctgcattttt atctgtaaaa gctttaggaa
     5401 ataaaaatgt cacctgtatt ttgatgccat ataaaacaag cagtaaagac agtttgacag
     5461 atgcttcgaa tgtagctaag atcctgaacg tagaatcaaa agtcattgat ataagcgaaa
     5521 ccgtagatac cttgattaaa aatattggtg ataaaaaaat atcaaatgta agaaaaggaa
     5581 atattatggc ccgcgtaaga atgatcattc tatacgatga gtcagctgca agtaattcac
     5641 ttgtcatagg tacaagtaat aaaacagaaa tactgctcgg atactctacc atttttggtg
     5701 attcagcatc agcaataaat ccaattggtg acctttacaa aactcatata agaggacttg
     5761 cccgtcattt aaatgtccct gaaaatatta ttattaaaaa accatctgcc gatctgtgga
     5821 aaggtcagac tgatgagaat gaactgggat tttcatatga agaaattgat aaatatttat
     5881 ttgacaaggt tgacgaaaga aggtcagcag aatatttaga aaatgctggc tatgaagata
     5941 attttatgaa aagaattaac tctcttatta tcagaagtca gttcaaacga cttccgcctc
     6001 tgattgcaaa agtttcaaac agaaccgtta acattgattt cagatacaac agagactgga
     6061 atacttaaat tttaattatg tttataaaaa acttactatt catattttca tttcttttcc
     6121 tttcatgcag ttcatactca aatgataaag gcggaatggt tagttctgca agtgacttag
     6181 ctacacaggt cggaattgag attatgaaaa atggcggcaa tgcagtagat gcagccgttg
     6241 gagtaggatt tacacttgct gtcgtttatc cacaggcagg aaatcttggt ggaggcggct
     6301 ttatgatcat tcatttaaat gataacggaa aattattaaa tacctctatt gattacaggg
     6361 aaaaagctcc gcttgctgca tataaggata tgtatcttga taaggacgga aatgtaatag
     6421 atgacctaag tactacagga agtcttgctg caggtgtgcc cggctcggtg gcgggaatgc
     6481 tttatgcttt agataaatat ggtacaaaaa gcagatcaga aattttgagt tacgcaatca
     6541 gtattgctga tacaggtttt tttatagaag aaggattggc agaagcttta aactcttatc
     6601 agtcagagtt ttcacagttt gcaggaacta tgaatatatt tggaggtaaa tttataaccg
     6661 gtgaaagatt aatccaaaaa gatcttgcaa atactctcaa agcagttagt caaaatggaa
     6721 gagacggatt ttataaaggt gaagttgctg aaaaaatagt tcagcttatg ataaattcaa
     6781 atgggatgat ctcttatgag gatcttgaaa agtatcagcc cgcagaaaga gatgtggtaa
     6841 aaggaactta cagaggatac gacatacttt ctatgggacc tccaagcagc ggtggaataa
     6901 gtttgatcta tcttttaaat attctagaaa attatgactt agcaaaaacc ggatatggaa
     6961 gttccgaaaa tattcagtta atgactgaag caatgcgtcg tgtatattca gatagaagcg
     7021 agtttatggg tgatgctgat ttcgtcaatg ttccgataga tgtcatgaca tcaaaggaat
     7081 atgcaaaaga cagaatgaag gattttgtta cagatcaggc aggaagaagt aaagtaatta
     7141 aagcgggcga tccctttgga aatgaaagta aagaaaccac tcattacagt gtcgctgacg
     7201 gaaacgggaa tcttgtttca gtaactacaa ctataaatga tgtatttgga aataaacagg
     7261 tagttgtcgg tgcaggattt tttctgaata atgaaatgga tgattttgta tcaaagccgg
     7321 gtgtccccaa tatttacggt ctcgtcggta atgatgcaaa tgcaattcag cccgagaaaa
     7381 gaatgctcag ctcaatgact ccgactattg tatttaagaa tgataagcca tatctggtag
     7441 ttggatctcc gggtggcgga aggatcatta cttctgtact tcaaactatt ataaatctga
     7501 tcgatcataa tatgagtctt gaagatgcaa ttgactctcc ccgatttcat catcagtggt
     7561 tgccggatga agttcagata gaaaacggaa tgttaaataa tgaagtcaaa aattttcttc
     7621 aaagcattgg ttatactttt caaagcataa gtgattttgg aagagtgaat gcaattttga
     7681 taagcgaaga cggaactatg acaggtcatt cagacagaag aggttacggc agctcagtag
     7741 gtttttaatt ttccaaaaaa gatttgagtg agatcaaaga tttaagactt aggggaaaaa
     7801 agaatatgat tcttgagctt tttttcaagt tctattttaa acactcattg aaaaggaatt
     7861 ttataatagt aaattttaaa ggaaaaaaat tgctgattct aaattaatta ttaacgacat
     7921 tctgaaaaaa accataaaca ttatcaataa cttttttttc atcatgatac atctcaaact
     7981 ttgaaatata tttatcacca aactctcccg attcaatatt tatctcattc tttaaaatga
     8041 ccttaaggtc attactttct atttctattg atttaatttt gtttgtgaaa aacgttttct
     8101 ttgttaataa tttaaaatct tccttttcta gcagaagtaa attgtctttg aagatttgca
     8161 atttcataaa cttattttcg ccttttgact ctgagacaaa aaatataaaa gaataatcat
     8221 ctctgataaa tttcccccag tttgaatatg aaattttgtt aaaagaattt gtgcttcccc
     8281 aggttttatc aatatatcct tttccatcaa aaatagtttt tactctttta taatctctgt
     8341 aaactttgat cttaccggat acatggcata aaggagaagg catccagcaa acttcttttt
     8401 ctaaattatg atcagttttc ttatttatag atgacttttt caataaaggt ttaaattcaa
     8461 attcggcttt aaatttgctt tcaagctctg ccgaagagta atttatattt aaaagatatt
     8521 tatcattctc cttatcatga caaaaactgt ttttatctaa aatcatttta actaagccat
     8581 cggattcctc tattctgaat tcttcttttt tataatcata aattgcatca tatatcaatc
     8641 tgttctgata atataaaaaa aatctgaacc tgcagtagtt attggtatta tctgaattgg
     8701 ggttaccatt tgaactgact ttttccggat tgccgtaata aattcttgcg gtaaaataat
     8761 aatcatcgtc attatctata gcatcaaaat accacatttc aaattctccg ggcttagaac
     8821 ttccagaatt gttaaatttt tctatatcag aatcgaattt caatatataa gatttttagt
     8881 tgacaatatt ttgattttta caatcaaaac aaatataaaa agaaattata ataaaatcag
     8941 aaatttaggg tttcaaaact taatttatat tttacaatag ttacaataat taaatttact
     9001 atattgctta acaatttgtg gaataggaat tttacagaat attaaacctt cagtttgaga
     9061 aaagtttcta agtttacgaa cattgaatat taaacttagt ataagtaatt tagcattcca
     9121 aatttattaa acattctata tgcatcaatt aaaaatttta acaattctct ttttttttat
     9181 aagcgttaat tccaatgctc aaatcgatac gaatattaat gtaataaaat atgatcttga
     9241 gtttgatctc attaatactt ttcaggaacc ttatccgaaa acttacagtg ctgtaaatgt
     9301 agtaagttta atggtcaact ctacagcagg acagataaca ttaaacgcag aaaattattc
     9361 acttattata gattcagttt cgggctcggg tcagactttc gttcacgaga atgatctttt
     9421 gaaaataaat ttagataaat tttataacag cggagatatg tttgatgtga aaatttattt
     9481 cagacataaa gaaatttttg acacggcata ttttgcattc aaaggtctta tgtatactga
     9541 ttctgagaca gcaggtgcca gaaggtggtt tccgtgtaaa gacatcccaa atgataaagt
     9601 gatgctcagg cttagtgcta gagttccgga aaaagttctt tttgtttcaa acggactgct
     9661 ggtagattcg gtttataaaa ataattctct tcattataca tgggagacaa atattcctat
     9721 ctcaacttat ctgatcgcaa tggctggaag taagaattac aaacttgaag tattcaactg
     9781 gaaaaaattg aaaccgcctt acgataatgt agaaataaga ctttactcac agccgggtga
     9841 aacgaaattt aactatgata acattaaaaa caaacttccg gaaatgcttg aagtattttc
     9901 aaaactttat ggcgattatc cttttgaaaa aattgctttt gttactacag acagaaattt
     9961 tccatggggt ggaatggaaa atcagacttt ggttacatta tgtccagact gctggtatga
    10021 aagtctgata gtccatgaat tagttcacca gtggtttgga gatatgatca caccgaatac
    10081 ctgggctgac atctggctga atgaaggctt tgctactttt aacgaagcca tctgggatga
    10141 atataataaa ggtcccaaag aatataaaaa gaatattcag aatgaagcta tgaaatatct
    10201 aagtggtgat ccgggctggg aattatacaa taaagactgg gctgtttcaa aaccaaatga
    10261 tgatgttgtg tttaattcaa atgttactta ttcaaaagca gcatctatag tttatatgct
    10321 aagatatgtt ttgggagatt cggttttctt tgatgcacta caggcttaca ccgataatcc
    10381 tcagttcaga tttgggaatg taacgaccag tgaatttcag gcagcagttg agcaggtcag
    10441 cgctcttgac ctggactggt tttttagtca atggcttatg aagccgggct ttccggaata
    10501 tgaatttaat tttactacag aaaaaagtaa aaatggaaaa tggaaaactg attatacaat
    10561 aaatcagatc cagtcaaata aaatttttta tcaaatgcct gtagaactta aagtgaaatt
    10621 tactgatgga agtgaacagc ttttaaagga aaggaattct tataattttc aaacctttaa
    10681 ttttgaattt gataaagagc cggaaagaat aatcttcgat ccaaataact ggattgtctt
    10741 aaaaaaagtt aaatagatca ttttcgcttt ttccataaag cagtaagccg ttttttaaac
    10801 tcggcttctt tgccgttttc agaaggcaga taataaatct tttcttttaa ctcttcaggc
    10861 agataatcct gttctgaaaa gttattttca taatcatgag aatatttata cccgcttccg
    10921 taattcagat ctttcattaa ttttgtcggc gcatttctaa gatgcattgg tacaccaaat
    10981 gccggaaaat tttcaacgtc ttcttttgct ttactgatcg ccaggtagca agcattactt
    11041 tttggagcac ttgccagata agcagcagcc tgagataaga caagagttgc ttcaggcatt
    11101 ccaataaaat cgacagcagt aaatgcactg acagcaatta caagagcctc cgggtctgca
    11161 ttcccgatat cttctgacgc gagcactatc atccttcttg caatgaattt cggatcttca
    11221 ccacctttca gcattctcgc catccagtat agcgcagcat ccggatcact tcctctgaca
    11281 ctttttatga atgctgaaat tatattataa tgctcttcct gatttttgtc atacttaata
    11341 taattagcct gaaaagcttc tttgaaaatt tcattagtta gttcaattac gccttcatca
    11401 tttttcgaag ttaattttac tgcaagctca agaccgttaa gaagttttct accatcacct
    11461 gaagacagac cgattagaaa atctttgtct tcaattttaa tatcatattc ttttaacaca
    11521 atatcatttg tcagagcatt attaattatt ttcaagaaat cattttttga taattcctcg
    11581 agaataaaca cacggctccg cgagagcaga ggagatatca cttcgaaaga aggattttca
    11641 gtggttgcac cgataagaat cacagtacct ttttcagaac tctgaagaag tgagtcctgc
    11701 tgggatttac tgaacctgtg aatttcatct atgaacaata ttgtttttct tttcttctgt
    11761 ctgagattaa attcagcttt ctctatcgcc gctcttacat cttttacacc tgaggaaatt
    11821 gctgacaatt gaataaattc tgaatttaca gatcttgcta tcagcaatgc aagtgttgtc
    11881 tttcccacac ccggcggtcc ccagaatatc attgaggatg gttcgttttt ttcgatcatt
    11941 gttctgaccg gtttacctgc agaaatgaga tgctcctgtc ctgcaaaatt ctcaattgaa
    12001 tcaggtctca ttctttcagc aagcggagcg ttatattctg tttccttatc agaaacattc
    12061 ccaaaaagat ccatagaatt caaataagtt tttctttctt tttaaaattt ttatttacta
    12121 aataaacacc gatcagaata attaacataa ttacaaaatg aatatgatat aaaacttccc
    12181 cgtccagtat tccccagatt attgatacga tcggaaggag atatgtcact gtcgttgcaa
    12241 agagagcatc agtgatctgg ataagtttag tataaagaat catggcaaaa gctgtgccga
    12301 ctattccgag aattgcgata taaaacattg ccagaccgct gcccggcaca gtattaaaaa
    12361 catggatgag atcagttgac aataaatata taagacaaac cggaccgact gttaataatg
    12421 atactcctgt tacaataact gaatttatat cagacagata taccttaatg agattaagac
    12481 tgattgaata acataaagta gccaggacga caagcatcac ataaatatta aaactgccaa
    12541 atacacctga ctcatctaca aatgcaagac tcatcactcc cagaaagccc agtaacatcc
    12601 caatgatctg catattcttt attttatatt taaaaataaa aacagctatc aacattgtaa
    12661 atattggagt aagtgcatta agtattccgg tgacagagct gggaagtaca gtttgagaat
    12721 aagcaaaaag tatgtaaggc aaaatattgc ctataattcc tattattaaa agaacaaaaa
    12781 gttttctccc cggtaatcga aatattttta gtactgctgc aggaagtaat actaaaaatc
    12841 caaaagtgat ccgcattgtt cctacctgca atggtgtata taccagtaaa ccttttttta
    12901 ttaatatgta agagcttccc catattacag acagtattat taaaagagac catgcaagga
    12961 aatcatgact tttactgaag cgacggctta tcaattataa ataattttat ttcattcaaa
    13021 ataatattaa tttagcaaaa caataatgaa tatcaaatta tgaaatttat tgtttaacaa
    13081 atgtttccaa atttttttat ataaagccag actctccttt gaaaaaaagg gaagagcaat
    13141 aactgtttta aaaatctttt tggttttggc tatgccgggg tatgttttac agcttgtctt
    13201 aatcctcatt agaagaacgg aaaaaataat tacactaaca taattttaaa acctaaagaa
    13261 tatttaatat tttttattta aaatttaata tttaatattc atctgcctat tgtattaaag
    13321 attcatttga attatgtttg aataataaaa atgagcggac tcaaacacaa aataattttt
    13381 catattgaca tggatgcttt ttttgcttcc tgcgaagagt caataaatcc gtctctccgc
    13441 agcaaggcac ttgtagtcgg cgggacgaaa gatgacaaga gaagcattgt cgcctgtccg
    13501 aattatcttg cccgcgcaaa aggtgtcagg actgcaatgc ctctttcgcg tgcaatactg
    13561 ctcgtgcccg atggaaattt cataagaagt acacgcggtc tttattctga ttattcaaaa
    13621 agggtccgtg agatattttt caaatacact cctgccgttc agccggtcag tgtggacgaa
    13681 gcttatcttg atgtaacggg agtattacat cttcataaga atgatcatat tttacttgct
    13741 gaaagaatta aagatgatat aaaggaaacg cttgatatta catgttctat tggaattgcg
    13801 gtgaacaaga tctgtgcaaa gatcgcctcg aagataaata aacctgacgg agtgactgtt
    13861 gttccttttg gaaatgaaaa agaattcctg agtaagatga caattgacaa gatacccggt
    13921 gttggaaaag caactcagga aagactgagg aaatatggaa taaatcttat aggtgatata
    13981 ttgaaattcg atcaaacctt ttatgataaa gagattggtt ttcatactgc attccttatc
    14041 aatgtagcaa acggcagagg cagtgataat gtaaaagaaa acagtgagga aagaaaatca
    14101 ctctctaagg aaaatacatt ttatgaggat gtgtctgaca gggagtttct gcagtctgag
    14161 ttatattctc tcatggaaag atgctgtcag aaactgagaa aagtaaaact gaaaggaaga
    14221 actgtcactg tgaaagttaa atattttgac ttcacagtaa atcaaagatc ttatactact
    14281 tccaaatatt caaatctcga gactgatttt tatgaagatg ctgtgagtct tcttgacaaa
    14341 atgctgatga ataaaaaaat ggtaagactt ctcggagtga agttttcaga gtttgtagcc
    14401 ggtgatgacg cagttcagga aaatcttttt gatgatgttg ataaaaaaga aaatcttctg
    14461 aacaagctcg atacgatcag gaagaaatat gattttgaaa ttgtaaaatt cggcaggaca
    14521 atgaaacaga aataagtttt attttttata taacatcata aaatcatcca tcacataacc
    14581 gctgccaata tccatatcaa ttaatttttc tttgtgtatt ttatatttat aatgttcaga
    14641 ccgtcgacat aaaatccttc gacattattt gcaagcagat atatcttttg attataataa
    14701 agatacagcc aagccgcatc gtctatcact atcttctgca ttttgtcata gatctttttt
    14761 ctttctgcaa agtcagttat cttcaatgca tcttcataaa gtttatctac ttcgggattt
    14821 gaatagccta cacggtttgg tccgtttggt gtaatatttt tgctgtaaaa aagtgccatg
    14881 aaattctcag gatcataata atccgcaccc cagcttgctc tccagaaagg aaatactccg
    14941 tcttcctgtt tggaaattaa tgtggactgt atcatctgat cgagtttaag gtcaatgccg
    15001 atctccttca gctgctgctg tactgctatt gcaagagtct tctgaatttc ttcattacct
    15061 gtgacaagtg tcagatcaag tccttttcct ccgggatatc cggcttcact taaaagcagc
    15121 atagctttat ctttatcaaa tttatatccc ttgatcgaag catcaaagcc cggcataccc
    15181 ggaggtatgg gaccattctc tgctgaggta cctctgtttt ttaatacaaa cttcaggatc
    15241 ctgtctctgt ccactgcata attcaaagcc ttacgcagct taagattatt tgtaaaaggt
    15301 ccttccttcc cgccgggaag atcaggcgac tgcatcattc caaaaaaaac tgtctgaagc
    15361 cagggctgtc gattcaattt atatttatct ctttgtgaat ctataagatt tccgttctca
    15421 tctgtgatct gatcataagt ttcggaagac ggatcgtgat agtcatactt accattttga
    15481 aaatcgagaa attcagtttc agatgactga gtaaatgtaa atttaattcc gtcaagataa
    15541 ggtattgatt tcccttcttt atctttttca cggtaagttt catttttcac aaaattcaga
    15601 tcctgatcga gattccagct tgcgaatttg aacggacctg ttccgaccgg attctgaccg
    15661 aatctgtcac cgtaaaattc aactgcttcc tttggataca catacccgta agtcatagta
    15721 agaatgctga gaaaaggtga aaagggtttt gtgagctcaa tgatcagagt agaatcatcg
    15781 ggagctttta atcctgatat accatctact ttcttatcgg cagtaccgga cataaaatct
    15841 atatattcct gcgcaccttt tatcctgtct ctgtaaaccc attgcccgcg tgactttgtc
    15901 tttggattgt taactctttc gatgcaatat ttaaaatcct gcgcagttac ttttctccct
    15961 tttccgcctg attctttaaa acattcattg tcatgaaaat acacatcgtc ttttaaatga
    16021 aaagtatatg tcagagcatc attggaaatt tcccagcgtg atgctattga cggcagaata
    16081 ttactttcct tatcaaactg tacaagtcct tccatcatca cagacaaacc ccatatcgac
    16141 tgtatggaat ttgacatgac aggttcaagt gtttctatat tctgatttaa attaatattg
    16201 aatacattac tttcggtttt agtcttttcg ttacatgaat agagaaatag tgaaaggaaa
    16261 aaaaatatat acagtgaaaa ttttttcatt taaagataca ttgtaattta taatgttaca
    16321 aaataaagga tttaagattt aaaaaataat gtaagaaaaa ataatttgta aactgtaaac
    16381 ataacatgga taaaatcaca catgataaca ttctgaaagc tcactgcttt aatgagcttt
    16441 ccgcaactga ctatttagta agttacagaa acattggaga gatattaaag gaaagacttt
    16501 ccgaaaagcc ggataaaatc tatatcactt tttatgatga taatggaaag atgacaagtc
    16561 tgacttacag agactttgta cagagagcat atcagcttgc cgatttcctt ttaaaagaag
    16621 gagttacaaa aggcagcaga gtcgcgacat tctcacataa tcatttcaaa acagttatac
    16681 tttattttgc aagctggaca atcggcgcag tagtagtacc ggtaaatgtg agcgaagaat
    16741 ccgacagagt agattacatt ctgaagaatt ccgaatcgaa aattgtgttt acacgtgaag
    16801 aatatatttc gaaattgaat aattcagatg ctgtaaaaaa tattaaagtt attccactcg
    16861 attctttttc atttgatctt tattcagatg aaccggatcc cgctgattta aattttgata
    16921 aggaagctga atgcttaata gtttatacat caggcacgac aggaaatcca aaaggagtag
    16981 tgctcacaca atataatctg atggctgatg catttgcaat ttcagaatgg cacggacttc
    17041 ataatgacga tgtgatgatg tgcgtacttc cgatccatca tgtaaacggc acggtagtta
    17101 ctgtcatgac gacaatgttt tacggaggga aattaatttt gaatcaaaaa tt
//
LOCUS       DAORVQ010000050        16904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1448_length_16904_cov_6.692089, whole genome shotgun sequence.
ACCESSION   DAORVQ010000050 DAORVQ010000000
VERSION     DAORVQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1448_length_16904_cov_6.692089"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PLX80_05920"
     CDS             <1..1304
                     /locus_tag="PLX80_05920"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQY20367.1"
                     /translation="VFTDDEKFSESQLPVIKVDDVRKFMAMVSGIFYGDPSSKLKLIG
                     VTGTNGKTTSAYLIHVMLSSAGYKTGLIGTIECMIGNEKIESKLTTPDSVELNMILNK
                     MVNEKTEFCVMEVSSIALSMERVYGLKFDTAVFTNLTSEHLDFHKDMENYFEAKKILF
                     DYLGEDSFAVSNKDDEFGQKILSDTKAEKVFYSVDSPIESDEDLKADDVKLGMDGINF
                     NMIYREKNVNISSDLAGRFNVYNILASAGTVLNYNVSLKSISDVLDNFNGVTGRFNRI
                     PLPNGAVAVIDYSHTSDSLKNAIEAAREIIKDENKNGSLITIFGCGGNKDRLKRPVMG
                     KYATELSDFTIITSDNPRFEEPFEIIEEIKKGISEEAEYKVIENREEAIKAGIGMSVT
                     GDIILICGKGHETYQEIKGVRSHFDDKETVLKYQNLSLKNA"
     gene            1297..2688
                     /gene="murF"
                     /locus_tag="PLX80_05925"
     CDS             1297..2688
                     /gene="murF"
                     /locus_tag="PLX80_05925"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQY20368.1"
                     /translation="MLELKDLLSIDSVRDYQEGKEIKNRFSGVSIDSRTVGKNEIYIA
                     IKGENLDAHKFLPAVFLKGIKLFVVNEKWFIKNKNKYKGNSIVAVNDTTEALGQFAAV
                     HRSRLNIPVFCIGGSNGKTTTKDLVSTVLDKKFKVLKTEGNFNNHIGLPLTLLRLNKS
                     HEIAVLEVGCNHFKEVEYLMQIAKPSFGMVTNIGREHLEFFKDLKGVAKAEFELYDYI
                     EKTSGTAFMNFDDKFIRSRSKEMSNAKVISYSYSSKSDVTGEFEGYNKMYQPVIKVTK
                     GAKSFNVTISTFGKHSVFNGLAAAAAGLSFGVSASSVKSALANFKESSSKRMEITDHN
                     GIKFINDTYNSNPESVRMGIETMMGFENKGEKYAVLSDMLELGKGSVSEHKEIGKFIK
                     RSGIKNLYTFGKYSIHTFKSAGKMENNLYFENKTDLAELLKRNLKAGDIVYVKGSRGM
                     KMEEVINHITEKK"
     gene            2692..3801
                     /gene="mraY"
                     /locus_tag="PLX80_05930"
     CDS             2692..3801
                     /gene="mraY"
                     /locus_tag="PLX80_05930"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQY20369.1"
                     /translation="MLYKLAEYINANFNPPGFDLFRFITFRSALSAITALLISFVLGP
                     RIIKFLKKKQIGEARKEDGPQFHWSKAGTPTMGGFIILLSVIIPVLLWGDLGNIYVII
                     ILFVTIALGIVGFTDDYLKVVKKYKKGLAERYKMLGQITVGLIVGLAIYFLPQFDGIH
                     SITTIPFLKGYEFDFSYAYIPIVVFIITATSNAVNFTDGLDGLAVGTVGIVALTLGII
                     AYVSGNVETSKYLNIIYLRGNGELAIYCTALVGAALGFLWYNFYPAQVFMGDTGSLAL
                     GGAVGTLCVLVKKEFMLPILGGIFFMEAISVILQRYYFKYTRKKYGEGKRIFKMAPIH
                     HHYEMKGIPEPKIVIRFYIIAVILAIITLASLKIR"
     gene            3808..5172
                     /gene="murD"
                     /locus_tag="PLX80_05935"
     CDS             3808..5172
                     /gene="murD"
                     /locus_tag="PLX80_05935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQY20370.1"
                     /translation="MTDKRSFTILGAGRSGIAAAGLLKKNGYEVFLSEENQEDSLKYF
                     NKEFLLKEKIEFETGGHSERIYDNDVFIVSPGIPPESEVILKAVRLGKKILSEIEVAS
                     EFCKCPIIAITGTNGKTTTAVLTGEIFRNAGYDVKVCGNVGLAFSEIVSDLKENSIVI
                     LEVSSFQLEYTENFRPDVSVILNITPDHIDWHKSFENYLYAKEKIRRNQTVENLSVLN
                     FDDKILRDELQNSNAAEAFFSIKNDIQESEIKRGCMIKNNRVIYFDKSKNINEIIMET
                     HEINIRGNHNLYNSLAALISARAFDIKKEIIKDTLIRFKGVEHRIEFVRELDGVKYYN
                     DSKATNIDSVIVALESFEKDLILILGGKESGNDYSVIDKLVSERVKIIVAIGESKNNI
                     IEHFSKIVKCESTESLDEAVKYCKVNSSQGDSVLLSPACKSFDMFDSYEHRGKEFKKA
                     VNNL"
     gene            5191..6303
                     /gene="ftsW"
                     /locus_tag="PLX80_05940"
     CDS             5191..6303
                     /gene="ftsW"
                     /locus_tag="PLX80_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQY20371.1"
                     /translation="MSHKYKIDVWILLPVLVLMGISVVAVYSASSTFAMDRFQDSNYL
                     IKQHMIKVGISIILIFLIAKFDYKNYRQFAKYLLWGTIILLILTLFIGNELKGASRWI
                     YLGPFSFQPSELARYFIIMYIALLLAKKKDYLDLLYRGYLPIIFYVLLVSGLILIQPN
                     LSVSLIIFATCMSMLFLSNVKFKHLVITLLSLVPLVVIFILSKDYIIKRIVSYSEHST
                     GGEGSYQLQQALIGFGNGGLLGIGPGNSNQREYFLPEAHGDFIFSVIGEEYGFIGTFL
                     IIALFFIILYRGYKIAKDTKEDFGKYMAFGITTMIVLNAVVNMAVASGVFPTTGVTLP
                     FVSYGGTSILFSAIAVGILLNISSFRDEINEEITLE"
     gene            6281..7399
                     /gene="murG"
                     /locus_tag="PLX80_05945"
     CDS             6281..7399
                     /gene="murG"
                     /locus_tag="PLX80_05945"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQY20372.1"
                     /translation="MKKSLWSRVMKYLFAGGGTGGHIFPAIAIADEIRKLDHEAEILF
                     VGAKGRIEEKIVPQNNYKLEVISISGFDRQNFFKNILLPVKVMTSITQSRKIIRDFSP
                     DVVIGTGGFVCGPVIYTAKRMGIPSLIQEGNSFAGKTIKILSEKSDKVVINFEETKNF
                     LKRKDNIIKISHPVRNSLSIRNREDSLLKFGLETKKKSLFIFGGSQGATAVNSALIKI
                     IPDLNKEDINIIWQTGENDFDKISEMLKELDHKIRILKFVNNMDEAYSAADLIICRAG
                     ITSIMEIALLKKAAILIPFPGAAEDHQVKNAMSLKKMNAAEVILQENIEKELLPGILR
                     LIRNDNALNELRNNVSEFSDRDAARKIAEEVLKLANKN"
     gene            7412..7879
                     /locus_tag="PLX80_05950"
     CDS             7412..7879
                     /locus_tag="PLX80_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20373.1"
                     /translation="MNKRAYKYKLDFYYKSLALYLITLVIYILIKGKFFQARFEVVVK
                     DPIIYIISIFIFFYLILLVLNALRGREIIFETDSIIFKNRFGQKVINLNEIIQIRFSR
                     EKKRMREERSDSRIIKLKLNHRRRMIRVKLSDFNDEKELMNEFKNISKNLTQK"
     gene            7879..9273
                     /gene="murC"
                     /locus_tag="PLX80_05955"
     CDS             7879..9273
                     /gene="murC"
                     /locus_tag="PLX80_05955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQY20374.1"
                     /translation="MDASENIFNNVKKVHLIGIGGIGMSGIAEYLVRKGFKVSGSDLN
                     PGSSGRKLEILGVKIFKRHDAENLPPDTELVIYTSAVNESNAEMKKATELNIKTIKRA
                     AALGNIVNEMFMIAVCGTHGKTTTSAMIAKILIDNKLDPTVFVGGSMEFLDGSSSKIG
                     KSNIAIVEADEYDRSFLTLKPDVVVITNIEADHLDIYKDIDDIKDTFKKFLDNGKDDM
                     KIIACGDDKIVSELIADYPKKITYGFDKSNDIVIEHMGYEKSSIVFHVDNENLRIKVF
                     GNHNVLNSTAAYAAANSFGIKSEGFNDSMKTFTGVKRRMELKYNNGIKVYDDYAHHPT
                     EVKVTLEAIRKMYEGRIITVFQPHLYSRTRDFYKEFAESFKDTDILFLAKIYPAREES
                     IPGVTSELILNEYTVLGKEGYYIDDNDKLLSKLESISKDGDVFIFQGAGDITELRSRF
                     VKEMKKNSNWTVPL"
     gene            9270..10043
                     /locus_tag="PLX80_05960"
     CDS             9270..10043
                     /locus_tag="PLX80_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQY20375.1"
                     /translation="MNIKKAILVIIFTALGTLAVLSLSWRSENPVEVISVSGNYSISR
                     EEILNYAHLNDTLINPESIGIDLIQDRISKHPEIKSVYVSLVQPSELKIEVVEKRPVA
                     ILNGESEMYLIDDGLDVFPFRNYSKLYDLPVISGVRIDMNPKSKNKFNTEDLRTALFI
                     ILNSYKDSKAMYNNISEIKLSDTNKLIIYLTEDSTPFYFPRYSDKKIADKSYQKELMD
                     KLKIFKNYLNQSLADQLKEKVNYIDLRFKNQIIVNSNNQ"
     gene            10043..11323
                     /gene="ftsA"
                     /locus_tag="PLX80_05965"
     CDS             10043..11323
                     /gene="ftsA"
                     /locus_tag="PLX80_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQY20376.1"
                     /translation="MAAKKKKKKKSDEDIIVGLDVGTTKICVIVAKVKDDKNIDILGI
                     GKAPSHGLHRGIVVNPTKTSESIKAAIEEAEMNSGIEITSASVGIAGHYIRCIQSNSA
                     IGINNPDRVIQEEDVIRLIEQARIIKLPSDTMIIDVIPQEYIIDGKDGIFENPVGMFG
                     TRLESKVNIITGLVSSMDDLSKCVESCGIEIDDIVLEPIASSHSVLDETEKKVGVAMI
                     DIGGGTSDIAVFKKGVLKYTAGIGIAGNLVTNDIVEALGITDEEAERIKREYGYATLS
                     EILNDDEIVVQSIRDIPKKTSKSILARIIQARMQDIFELAAIEIQNSQISKELHAGVV
                     ITGGGSLVKGTKELAEGILGMEVNLGIPTGLTGGLIKEVESPIFATGVGLVLHRIKTM
                     NEDSKINDFKKKRKGGSLTMNGFLKKVKTWFDEL"
     gene            11400..12662
                     /gene="ftsZ"
                     /locus_tag="PLX80_05970"
     CDS             11400..12662
                     /gene="ftsZ"
                     /locus_tag="PLX80_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQY20377.1"
                     /translation="MAIRIADSLNSGAKIRVVGVGGGGGNALNSMVNKGIEGVEFIAI
                     NTDAQVLEKNKAETKIQIGRNLTKGLGAGMVEEIGYKAVEESREEIEAALKGSDMVFV
                     TAGMGGGTGTGGAPAVARIAKGLGSLVVTIVTKPFKFEGKQKMLLAERGIEKLKNEID
                     SLIVIPNENILKLVSKEATSKEAFEVADRVLHNAVSGISKIITETGEINVDFADVKTI
                     MKDMGDAMIGTGIASGKDRAIQAAEDALLNPLLDEIKIAGSKCVLTNICANGLKVGEI
                     EIINEIIQKAAGEDAKFIMGWVEDENMGDDIMVTLIATGFNQNAKVNIPLDEVGAIAD
                     SRVNVNKLENSYAGYSNKGRISTLPNHEELPIYDEPAYTRRSVDLTDDLSEKRIKPPA
                     EIDEDLDFNDIELTDDFEKPAFLRRSAD"
     gene            complement(12758..14770)
                     /locus_tag="PLX80_05975"
     CDS             complement(12758..14770)
                     /locus_tag="PLX80_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20378.1"
                     /translation="MKIPNKLILIFIFTFAFSISLKSNPINQFINTVSPPMNANSVVK
                     SSDIVITFAQLMNGSLMTSDNIKIFGYQTGLMTASLDYNSVSNTLTINPVNEFKNGEN
                     ISLTLTSGLKTILNESISPFVYSFSVKALGGTGIFTKTSEINSNSDYVRSGDIDSDGD
                     IDLLINEKVYKNDGSANFSYYSSIPISGYTLLADFDNDGDLDILVTVNNVNYFFRNNG
                     LGDFTQTSTFPGSLHSYGDLDGNGFFDVTYFSPQSAKEINIAKNILGNFLFDTTYNVD
                     FNLPCNPHPDFDKRILIEDMTNNGMLDLVLINGNAGGGILNGYDFCQIYSLLKNNGSG
                     KFLPELLFTHSENGLPYWVTNIGDPHLFDKNNDGLIDIISPGLIIENNGNGLFTIAGM
                     LNAFSTTIDGDFNGDTKIDLLAQIFGVSPLLTHLNDGTGNFQDYFIGSNSFYGKGHTT
                     ADLDNDGDLDIVFETSDSKAAIHLNGDSPLPVELASFTSSVNLNSVKLNWSTSLEQNN
                     SGFEIQRSDNDAAGPEAWKKAGFVNGAGNSGTQINYTYEDKNLSSGKYKYRLKQIDFN
                     GGYEYFMLTSEAVIGIPSSTELQQNYPNPFNPVTNISYRLSESGFVTLKVFDNSGREV
                     KTLVNEYREAGYYKAVFDGSGLASGIYFYKLSADNFNQTKKLSLVK"
     gene            complement(14820..16496)
                     /locus_tag="PLX80_05980"
     CDS             complement(14820..16496)
                     /locus_tag="PLX80_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20379.1"
                     /translation="MKNHITAFKNACLLACLLACLSFIFPTNSFSQLTYPVRYPTAVI
                     PHNSDGNRLFFLYMPMIYEDNAGQDTFKMYYLNGNSWDKRYLARSTDYGITWTDTQRV
                     FTDSMYESIVLNPDSMKMFMGYNDWRIVSGTNRSFFYSMVSTDGGFNFSNKTKVMELG
                     EDKSFIWNEDTNEYWGYVRPYNIEPSCCFTSGCVSFGDGVRKIALMKNSIYFPNTLNW
                     SNPNVIVEIDTLEYINPASPDYRTQTYYMQVFRNGEDWWGLVGMYRVGNNGGENNNWP
                     HTHPEYTSDVELMWSDNGEDWHRTNNRQPFMALHDSINTIYSVGTIVGDSVYFYSAES
                     TILHSFYFTQNCVYSTRQFSEINGKYYSIYLSKMSIDKLNEWRPPSVVNINCAVEGFL
                     NTGTGKHNLRDTLSAQLRNSASPYGIASEVKSVIDSVTFLGNFIFPHVSPGNYYVVVF
                     GRNSLETWSANTISISNGVDANYDFTTGYSAAYGGNLVNVGSEYCIFSGDLDNDNLID
                     FSDEVIVDNDIFFSVTGYVNSDVNGDFTVDLSDAAIVENNSSNLVMTISP"
BASE COUNT         6064 a         2474 c         3401 g         4965 t
ORIGIN      
        1 tggtttttac tgatgatgaa aaattttctg aatcacaatt gcctgtaata aaggtagatg
       61 atgtaagaaa attcatggca atggtgagtg gcatatttta tggagaccct tcatcaaaat
      121 taaaactaat cggagtgaca ggaacaaacg gaaaaactac ttcggcttat ctaatacatg
      181 tgatgcttag tagtgcaggt tacaaaacag gactcattgg aacgatagaa tgtatgatag
      241 gaaatgaaaa aatagaatca aagttgacta cacctgactc agttgaactt aatatgatac
      301 tgaataaaat ggtaaatgaa aaaacagaat tttgtgtaat ggaagtttct tcgattgctt
      361 tgtcgatgga aagggtatat ggtttgaaat ttgatacggc agtatttaca aatctgacaa
      421 gtgagcatct ggattttcat aaggatatgg aaaattattt tgaagcaaag aaaatattgt
      481 ttgattattt aggtgaagac agctttgcag tttcaaataa agatgatgaa tttggtcaaa
      541 aaattttaag tgatacgaag gcagagaaag ttttttattc agttgatagt cctatcgaaa
      601 gtgatgaaga tttgaaagct gatgatgtaa aattaggaat ggatggaatt aattttaata
      661 tgatctacag agaaaaaaat gtaaatataa gttctgatct ggcaggcaga ttcaatgtct
      721 ataatatact tgcttcggca gggacggttt taaattataa tgtctctctt aaaagtatat
      781 cagatgtgct ggataatttc aacggagtaa ctggaagatt taacagaatt ccacttccaa
      841 acggagctgt agctgtaatt gattattctc atacatcgga ttcattaaag aatgctattg
      901 aagcagcgag agaaataatt aaagatgaaa ataaaaacgg atcactgatc acgattttcg
      961 gatgcggcgg aaataaagac agactgaaaa gacctgtgat gggaaagtat gcgactgagc
     1021 tttcggattt tacaattatc acttccgaca atccgagatt tgaagaacca tttgaaatta
     1081 ttgaagagat taaaaaagga atttcagaag aagcagaata caaagtgatc gaaaacagag
     1141 aagaagcgat aaaggcaggg ataggaatgt cagtaacggg agatattatt ttgatttgcg
     1201 gaaaaggaca tgagacatat caggagatca aaggagtaag aagtcatttt gatgataaag
     1261 aaacagtatt aaaatatcag aatctttctt tgaaaaatgc ttgaactaaa agatctgcta
     1321 agcatagatt ctgtaagaga ttatcaggaa ggtaaagaaa taaaaaatag attttcagga
     1381 gtttcaattg attcaagaac tgtcggtaaa aatgaaatat acattgcaat caaaggggaa
     1441 aatttggatg cgcacaaatt tttaccggca gtatttttaa aaggaattaa gctatttgtt
     1501 gtaaatgaaa aatggttcat caaaaacaaa aataagtata aaggaaattc gatagttgca
     1561 gtaaatgata caacagaagc gcttggtcag tttgcagcgg tgcacagaag cagattaaat
     1621 attcctgtct tttgtatcgg cggcagcaac ggcaagacga ctacaaagga tctggtgtca
     1681 acagttttag ataaaaagtt taaagtactg aagacagaag gtaatttcaa taatcacatc
     1741 ggacttccgc tgacattatt gagattaaat aagtcacatg aaatagcagt attagaagta
     1801 ggctgtaatc attttaaaga agtggaatat ctaatgcaga ttgcaaaacc aagttttgga
     1861 atggtgacaa acataggcag ggaacatctt gaatttttta aagatctcaa aggtgtggca
     1921 aaagctgagt ttgaattgta tgactatata gaaaaaactt cgggtacggc ttttatgaat
     1981 tttgatgata aatttatcag gagtagatca aaagagatga gtaatgcgaa agtgatttca
     2041 tattcttaca gttctaaatc tgatgttaca ggtgaatttg aaggttacaa taaaatgtat
     2101 cagccggtaa taaaagttac caaaggagca aagtcattta atgtcacgat atctacattt
     2161 ggaaagcatt cggtattcaa cggactagca gcagcggcag cgggattaag tttcggggta
     2221 agtgcaagtt ctgtcaaaag tgcattagct aatttcaaag agtcatcttc aaagaggatg
     2281 gaaattacag atcataacgg aattaaattt atcaatgaca catataacag caatccggaa
     2341 tcagtgagaa tgggaattga gacaatgatg ggatttgaaa acaaaggtga aaaatatgca
     2401 gtgttatcgg atatgcttga gcttggaaaa ggcagtgttt ctgaacataa agaaattggt
     2461 aaatttataa aaaggtcggg aataaaaaat ctctacactt ttggaaaata ttcaattcac
     2521 actttcaaaa gtgcaggcaa aatggaaaat aatttatact ttgaaaataa aactgatctt
     2581 gcagaattac ttaagagaaa tttgaaagcg ggagatatag tgtatgtaaa aggttcgcgc
     2641 ggcatgaaga tggaagaagt aattaatcat attactgaaa aaaaataaag tatgctttac
     2701 aaattagcag aatacatcaa cgcgaatttt aatcctccgg gttttgatct gttcagattc
     2761 attacattca gatcagcgct ttctgctatt acagcattac ttataagttt cgtacttggt
     2821 ccgaggataa taaaatttct gaaaaagaaa cagatcggtg aagcacgaaa ggaagacgga
     2881 ccacagttcc actggtcaaa agccggtacg ccgacaatgg gcggttttat aattttactt
     2941 tcggtgatca taccggtttt attatgggga gatcttggaa atatatatgt gataataatt
     3001 ttgtttgtga caattgcact tggcatagtt ggatttactg atgattattt aaaagtagta
     3061 aagaaatata aaaagggact tgcagagaga tataaaatgc tgggtcagat cacagtcgga
     3121 cttatagttg gactggcaat ttattttctg ccgcagtttg acgggattca ttcgatcacg
     3181 accattcctt ttttaaaagg gtatgagttt gatttttctt atgcatatat accgatcgtt
     3241 gtatttatta ttactgctac atcgaatgca gtaaacttta ctgacggatt ggacggactg
     3301 gcagtcggta cagtaggaat agtagctctg acacttggta tcattgcata tgtttcggga
     3361 aatgtcgaga catcaaaata tctgaatata atttatctca gaggtaacgg cgagcttgca
     3421 atctactgta cagcactagt cggagcggca cttggatttc tctggtataa tttttatccg
     3481 gcgcaggtat ttatgggaga cacaggttca ctggcacttg gaggagcagt cggtacattg
     3541 tgtgtgctgg taaaaaaaga attcatgctg ccgatacttg gcggaatatt ttttatggaa
     3601 gcaatatcag taatactgca gagatattat tttaaataca cgagaaaaaa atacggtgaa
     3661 ggaaaaagaa tttttaaaat ggcgccaata catcatcact atgaaatgaa aggaatacct
     3721 gagccaaaga tagtgatcag attttatata attgcagtga tacttgcaat aattacttta
     3781 gcgtcattaa aaatcagata aaatgtaatg acggataaac gttcttttac aatactcggt
     3841 gcaggcagaa gcggaatagc agcggcagga cttttaaaaa aaaacggtta tgaagtattt
     3901 ctgagtgagg aaaatcaaga agattctctg aaatatttta ataaagaatt tcttttaaaa
     3961 gaaaaaatcg aatttgaaac cggcggacat tcggaaagga tctatgacaa tgatgtattc
     4021 atagtcagtc cggggatacc acctgaaagt gaagtgatat taaaagctgt cagacttggg
     4081 aagaagattc tgtctgaaat tgaagttgct tcggaatttt gtaaatgtcc gataatagca
     4141 attaccggta caaatggtaa aacaacaacg gcagtactta caggtgaaat attcagaaat
     4201 gccggctatg atgtaaaggt ctgcgggaat gtcggtttag cattttctga aatagtgagt
     4261 gatctgaaag aaaattctat agtgatactt gaggtgagca gttttcaatt agagtacacg
     4321 gaaaatttca gacctgatgt ttcggtgatt cttaacatta ctcctgatca tattgactgg
     4381 cataagagct ttgaaaatta tttatatgca aaggaaaaga tcagaagaaa tcagacagtg
     4441 gaaaatcttt ctgttttaaa ttttgatgat aagatactaa gagatgaact tcaaaattca
     4501 aatgcagcgg aagcattttt cagtataaaa aatgatattc aggagtcaga aataaaaagg
     4561 ggatgcatga taaaaaacaa cagagtaatt tattttgaca aaagtaaaaa tataaatgaa
     4621 attataatgg aaactcacga gatcaacata agaggtaatc acaatcttta caactcactt
     4681 gctgcattaa tttctgcaag ggcttttgat ataaaaaagg aaatcatcaa agataccctg
     4741 ataagattca aaggtgtcga gcaccgaatt gaatttgtaa gggagctcga cggtgtgaaa
     4801 tattataacg attccaaagc taccaatatc gattcggtca tagtagcgct tgagtcattt
     4861 gaaaaagatc tgattctgat acttggcgga aaggaaagtg gaaatgatta ttctgtgatc
     4921 gataagctgg tttcagagag agtgaagatt attgtagcaa taggtgaatc gaaaaataat
     4981 attattgagc atttttcaaa aatagtaaaa tgtgaatcca cagaaagttt agatgaagct
     5041 gtaaaatatt gcaaagtaaa ttcatcacag ggagattcag tactgctgtc gccggcttgt
     5101 aaaagttttg atatgtttga cagctatgag cacaggggaa aagagtttaa gaaagctgta
     5161 aataatttat aaaaagttaa gtaataataa ttgtcacata agtataaaat agatgtctgg
     5221 atattactgc ctgttctggt actgatggga atcagtgtgg tagcggttta cagcgcgagt
     5281 tctacatttg caatggacag atttcaggac agcaattatc ttattaagca gcatatgata
     5341 aaagttggga tttcaatcat acttattttt cttatagcaa aatttgatta taaaaattac
     5401 agacagtttg cgaaatattt attgtggggt acaataatac ttttgatttt aactttattc
     5461 attggcaacg aattaaaagg cgccagtcgc tggatatatc tgggaccatt tagttttcaa
     5521 ccgtctgagc tggcaagata ttttattata atgtacatcg cacttttgct ggcaaagaaa
     5581 aaagattatc tcgatctatt atacagggga tatctgccaa taatttttta tgtactgctt
     5641 gtatcaggac tgatcctcat acagccgaat ctttcagttt cgctgatcat atttgcaaca
     5701 tgcatgtcga tgcttttttt atcgaatgta aaattcaaac acttagttat tacattactt
     5761 tctcttgtac cgctggtggt aattttcatt ctttcaaaag attatataat aaaaagaatt
     5821 gtgtcatatt cagaacatag tacaggcgga gaaggaagtt atcagcttca gcaggcactc
     5881 attggatttg gaaacggagg actgcttggc ataggtcccg gaaattccaa tcagagggaa
     5941 tattttttac cggaggcaca tggtgatttt attttttcgg tgatcggtga agagtatgga
     6001 ttcatcggca catttctgat catagcttta ttttttataa ttctttatcg aggatacaaa
     6061 atagcgaaag atacaaaaga ggattttggg aaatatatgg catttggtat tacgacaatg
     6121 atcgtactga atgcagtagt aaatatggca gtggcaagcg gagtatttcc gactacaggt
     6181 gtgactcttc catttgtaag ttacggcgga acttcgatct tattcagcgc gatagcagtc
     6241 gggattttac tgaacatatc ttcattcagg gacgagataa atgaagaaat cactctggag
     6301 tagagtaatg aaatatttat ttgcaggggg cggtacaggc ggtcacatat ttccggcgat
     6361 agcaattgcc gatgagatca gaaaacttga tcatgaagct gagatacttt ttgtaggagc
     6421 taaaggcagg atcgaggaaa agattgtacc acagaataat tataagcttg aagtaatttc
     6481 aatatcagga tttgacagac aaaatttttt taaaaatatt ttattaccgg taaaagtaat
     6541 gacatcgatc acacaaagca gaaagataat aagagacttc agccctgatg ttgtcatagg
     6601 cacaggcgga tttgtatgtg gaccggtcat ttatacagct aagagaatgg gaattccctc
     6661 attgatacag gaaggaaatt catttgcagg taaaacgata aagatacttt ctgaaaagtc
     6721 tgataaagtt gtgatcaatt ttgaggaaac aaaaaatttt ctaaagagaa aagataacat
     6781 aataaagatc tcgcatccgg taagaaattc tctgagcata aggaacaggg aagattccct
     6841 tcttaaattc ggattagaga caaagaagaa atctcttttt atttttggcg gtagtcaggg
     6901 tgcaacagca gtaaattctg cattaataaa aataatacct gatctgaata aagaagatat
     6961 caatatcatt tggcagacgg gtgaaaatga ttttgataag ataagtgaga tgcttaaaga
     7021 gttagatcat aagatccgaa tattgaaatt tgttaacaac atggatgaag catattctgc
     7081 agcagacctg ataatctgcc gtgcagggat tacaagtata atggagatag ctttattaaa
     7141 aaaagcagct atacttatac cattccctgg agcagcagaa gatcatcagg taaaaaatgc
     7201 aatgagtctg aaaaaaatga atgcggcaga agtgatactt caggaaaaca ttgaaaaaga
     7261 attgcttccc ggtatcctta gacttataag aaatgacaac gctttgaatg aactaagaaa
     7321 taatgtaagt gaattttctg atagagatgc tgccagaaaa atagccgaag aagttttaaa
     7381 acttgcaaac aaaaattgaa taacttaata aatgaataag agagcatata aatataaact
     7441 ggatttctat tacaagtcat tagcgctgta tctaatcacg ctggttattt atatacttat
     7501 caaaggaaaa ttttttcagg caagatttga agtggttgta aaagatccga taatttatat
     7561 aatttcaata ttcattttct tttatttgat tctgcttgtt cttaatgcgc tcagaggcag
     7621 agagattatt tttgaaacag acagtattat atttaaaaac aggtttggtc aaaaagttat
     7681 aaatctaaat gaaattattc agatcagatt ttcaagagag aaaaaacgga tgagagaaga
     7741 aagaagcgat tcaagaataa ttaaattaaa actgaaccac agacggagaa tgataagagt
     7801 aaagcttagt gattttaatg atgagaagga attgatgaac gaatttaaaa atatttcaaa
     7861 aaatttaaca caaaaataat ggatgcatct gaaaacattt ttaataatgt aaagaaagtt
     7921 catctgatag gtattggcgg gataggaatg agcggaatag ctgaatatct tgtacgtaaa
     7981 ggatttaaag tttcgggtag tgaccttaac ccgggttcgt ccggaagaaa gttagagatt
     8041 ctgggtgtga aaattttcaa acggcatgat gcagaaaatc tgccgccgga tactgaactt
     8101 gtaatttaca catcagcagt aaatgaaagt aatgctgaaa tgaaaaaggc aactgagctg
     8161 aatatcaaaa ccattaaaag agcagcagct cttggaaata ttgtaaatga aatgtttatg
     8221 atagcagtct gcggaactca cggaaagact acgacttcgg ctatgattgc aaaaattttg
     8281 atagacaata aacttgatcc gacagtcttt gtcggaggga gtatggaatt tcttgacgga
     8341 agctcatcta agatcggcaa aagtaatatt gcaattgtag aggcagatga atatgacaga
     8401 agttttttga ctctgaagcc ggatgttgta gtaataacaa atattgaagc agatcattta
     8461 gatatatata aagacattga tgatataaaa gatactttca aaaaatttct tgataatgga
     8521 aaagatgata tgaagatcat tgcctgtggt gatgataaaa tagtatcgga gcttattgct
     8581 gattatccta aaaaaattac ttatggattt gataaatcga atgatatagt gattgaacat
     8641 atgggttatg aaaagtcaag tatagttttt catgtagata atgaaaacct gaggattaag
     8701 gttttcggaa atcataatgt tttaaattca actgcagctt atgcagcagc aaatagtttt
     8761 ggaatcaagt ctgaaggatt taatgacagc atgaaaacat ttaccggtgt taagagaaga
     8821 atggaattga aatacaataa cggaataaaa gtatatgatg actatgcgca tcatccgact
     8881 gaagtaaaag ttacactgga ggcaatcaga aaaatgtatg aaggcaggat cattacagta
     8941 tttcagcctc atctttattc gaggacaaga gatttttata aagagtttgc tgaatcattc
     9001 aaagatacgg atattctgtt tctggcaaaa atatatcctg cacgggagga aagtattccg
     9061 ggagttacaa gtgagctgat cttaaatgaa tatacagtgc ttggaaaaga gggatattat
     9121 attgatgata atgataaact actttcaaaa ctagagagca tcagtaaaga cggtgatgtt
     9181 tttatatttc agggtgcagg cgatatcaca gagttaagaa gccggtttgt aaaggagatg
     9241 aaaaaaaatt ctaactggac agtaccgttg tgaatattaa aaaagcaata ttagtaatca
     9301 tatttactgc acttggaacg ttagccgttt tatcgttaag ctggagaagt gagaatcctg
     9361 tggaagtaat ttctgtatct ggaaattatt caatctccag ggaagagata ttgaactatg
     9421 ctcatctgaa tgatactttg ataaatcctg aatcaatcgg gatagatctg atacaggaca
     9481 ggatctccaa acatcctgag atcaagagtg tttatgttag tttggtacaa ccatcagagc
     9541 tgaagatcga agtagttgag aaaagacctg ttgcaattct taatggagaa agtgaaatgt
     9601 atctcattga tgacggactt gatgtgtttc ctttcagaaa ttattcgaag ctgtatgatc
     9661 ttcctgtgat cagtggagtc aggattgata tgaatccgaa aagtaaaaat aaatttaata
     9721 cagaagatct gaggacagct ttatttatta tattgaattc atataaagat agtaaagcga
     9781 tgtacaataa tatttcggag ataaaacttt ctgatacaaa taaattaata atttatctga
     9841 cggaagattc tactccgttt tattttccaa gatatagtga taaaaaaatt gctgataaaa
     9901 gttatcagaa agagcttatg gataaattga aaatttttaa aaattattta aatcagtcac
     9961 ttgcagatca actgaaagaa aaagtaaact atatagatct tcggtttaag aatcagataa
    10021 ttgtaaattc aaataaccaa taatggcagc taaaaaaaag aaaaagaaaa aatccgatga
    10081 agatataata gtgggtcttg atgtaggcac tacaaagatc tgcgtaatag tagccaaagt
    10141 aaaagatgat aaaaacatcg acatactcgg gattggcaaa gctccgtctc acggacttca
    10201 cagaggtata gtagtaaatc ctacaaagac ttcagagtca attaaggcag ccattgaaga
    10261 agcggaaatg aattcgggaa ttgaaattac atcagcttcg gtgggaattg caggtcatta
    10321 cataagatgt attcagtcaa acagcgcgat aggaatcaat aatcctgaca gagtgataca
    10381 ggaagaagat gtgataagac taatagaaca agcaaggatc attaaacttc caagcgacac
    10441 aatgatcatt gatgtgatcc cgcaggaata tatcatagac ggaaaagacg gaatttttga
    10501 aaatcccgtt ggaatgttcg ggacaagatt agagtctaaa gtaaatatta taacaggact
    10561 tgtatcttca atggacgatc tttccaagtg tgtagagagc tgcggaattg aaatcgatga
    10621 cattgtactg gagccgatcg cttcttcaca ttcagtgctt gatgaaaccg aaaagaaagt
    10681 cggtgtagca atgatagata ttggtggtgg aacttctgat atagcagttt ttaaaaaagg
    10741 agttctgaaa tatacagccg gtataggaat cgcaggaaat ttagtaacga atgatatagt
    10801 ggaagcactg ggaattactg atgaggaagc cgaaagaata aaacgtgaat atggatatgc
    10861 gactttatct gaaattttaa atgatgatga aatagtagtt cagtccataa gggacattcc
    10921 aaagaagaca agcaaaagta ttcttgcacg gatcatacag gcgcggatgc aggatatatt
    10981 tgaacttgca gcaattgaaa ttcagaattc acaaatatca aaagagcttc atgcaggagt
    11041 tgtaattacg ggtggaggtt cgctggtaaa aggaactaaa gaacttgcag aaggaatact
    11101 cggaatggaa gtgaatctcg gaattccaac cggtctgaca ggcggattaa taaaggaagt
    11161 ggagagtccg atatttgcga caggagttgg acttgtgctg catagaataa agaccatgaa
    11221 tgaggacagt aagataaatg atttcaaaaa gaaaagaaaa ggtggatctc ttactatgaa
    11281 cggattttta aaaaaagtaa aaacatggtt tgatgagctg taaaaatttg taaaagagaa
    11341 ttaaaaattt tatataaagt aaaaatctta ttaattaaaa taagaaaggg taaattaaaa
    11401 tggcaataag aatagctgat tcactgaaca gcggcgcaaa gatcagggtc gtaggagtgg
    11461 gaggcggagg cggaaacgct ttgaattcca tggtaaataa aggtattgaa ggagtagagt
    11521 tcatcgcaat aaatactgat gctcaggtcc ttgaaaaaaa caaagctgaa accaaaatac
    11581 aaatcggcag aaatcttaca aaaggtctcg gcgcaggtat ggtggaagag attggttaca
    11641 aagcagtgga agaaagcaga gaagaaattg aagctgcatt aaaaggcagc gacatggtat
    11701 ttgtaacagc aggaatgggc ggcggaacag gtactggcgg tgctcctgca gtagcaagga
    11761 tcgcaaaagg tctgggttca cttgtcgtga caatcgttac caaaccattt aaattcgaag
    11821 gcaagcaaaa gatgttactt gccgaaagag gaattgaaaa actgaaaaat gagatcgaca
    11881 gtctgatcgt gattccgaat gaaaatatac tgaagcttgt aagtaaagaa gcaaccagta
    11941 aggaagcatt tgaagtcgca gacagagtac tgcataatgc tgtcagcgga atttcaaaga
    12001 tcatcacaga gacaggagag atcaatgttg actttgcaga tgtaaagaca atcatgaaag
    12061 acatgggaga tgcaatgatc ggaacaggaa ttgcgagcgg aaaagacaga gcaatacagg
    12121 cagcagaaga tgcgctgctg aatccgcttc ttgacgagat aaagatcgcc ggttcgaaat
    12181 gtgtccttac aaatatatgc gccaacggat taaaggtagg cgagatagaa atcattaatg
    12241 agatcataca aaaagcagca ggtgaagatg caaaatttat tatgggatgg gtagaagatg
    12301 aaaacatggg agacgatata atggtgactc ttattgcaac aggtttcaat caaaatgcca
    12361 aagtaaatat tccgcttgat gaggtgggag caatagcaga tagcagagta aacgtcaata
    12421 agcttgaaaa cagctatgca ggttacagta ataaaggcag gatatctaca ttaccaaatc
    12481 acgaagaact tccgatatat gatgaaccgg catataccag aaggtctgtc gatctgactg
    12541 atgatctgag tgaaaaaaga attaagccgc cagcagaaat tgatgaagac ttagatttta
    12601 atgatatcga acttactgat gattttgaaa agcccgcatt tttgagaagg tcagctgatt
    12661 aaatctttca aaaatacaat ttaattatcc cttatattaa aacagcagtc tgaaagtgac
    12721 tgctgttctt aaaattttaa attatgaatt cttaaattta tttaaccagc gacagctttt
    12781 ttgtctggtt aaaattatcc gccgagagct tgtaaaagta aatcccgctt gcaagtccgc
    12841 tgccatcaaa tactgcttta taatatccgg cttccctgta ttcatttaca agcgttttta
    12901 cttctcttcc cgaattgtca aatactttta atgtaacaaa accgctttct gaaagtctgt
    12961 aggaaatatt cgtaacggga ttgaaaggat tcggatagtt ctgctgaagc tccgttgatg
    13021 aaggaatgcc tatcactgct tcgcttgtca gcatgaaata ttcatatccg ccgttgaagt
    13081 cgatctgctt cagacggtat ttgtattttc ctgatgaaag atttttgtct tcgtatgtat
    13141 aattaatttg cgtgccggaa tttcccgcgc cgtttacaaa gccggctttc ttccacgctt
    13201 ccggaccggc ggcgtcatta tccgatcttt gaatttcaaa tccggaatta ttttgttcca
    13261 gagaagtgga ccagttaagt tttacggagt ttaaatttac gctcgaagta aatgatgcaa
    13321 gctccacagg caaaggactg tctccgttga ggtggattgc ggcttttgaa tcacttgttt
    13381 caaatacaat atctaaatct ccgtcattat ccaaatcagc agtagtgtgt ccttttccat
    13441 aaaaagaatt acttcctata aaataatctt ggaaatttcc ggttccatca tttaaatgcg
    13501 taagtagtgg agatactcca aatatttgag ctaataaatc aattttagtg tcaccgttaa
    13561 aatctccatc aattgtagta ctaaacgcat ttaacattcc agctatagta aataatccat
    13621 taccattatt ttcaattatt agtccgggag aaattatgtc aatcaacccg tcattattct
    13681 tatcaaataa gtgtggatct ccaatattag ttacccaata aggtaaacca ttctcactgt
    13741 gagtaaacaa tagttcaggc aaaaatttac cagatccatt atttttaagt aaagagtata
    13801 tttgacagaa atcataacca tttaaaatac cgcctccggc attcccattt attaaaacca
    13861 aatctaacat tccgttgttc gtcatatctt cgattaaaat tcttttatca aaatcaggat
    13921 gagggttgca tggtaaatta aaatcaacat tataagttgt gtcaaataaa aaattaccga
    13981 gtatgttttt ggctatatta atttcctttg cgctttgagg tgaaaagtaa gtaacatcaa
    14041 aaaacccatt cccgtcaaga tcaccataac tgtgaaggct acccggaaaa gttgaagtct
    14101 gagtaaaatc tcccagaccg ttattgcgaa aaaaataatt tacgttatta acagttacta
    14161 atatgtctaa gtctccgtca ttgtcaaaat ccgccagcaa agtatatccc gaaattggta
    14221 ttgaagaata atatgaaaaa tttgcagacc cgtcattttt ataaactttt tcattgatga
    14281 ggagatcaat atctccgtct gaatcaatat ctccggatcg gacataatca ctattggagt
    14341 ttatctctga cgttttagta aaaatccccg tcccgccaag agcttttaca ctgaacgaat
    14401 acacgaaagg tgaaatactt tcatttaaaa ttgtttttaa tcctgaagta agagtaaggc
    14461 ttatattctc accgttctta aattcattta caggatttat tgtcagtgta tttgaaacgg
    14521 aattataatc caatgatgct gtcataagtc ccgtctgata tccgaatatt tttatattat
    14581 cggaagtcat tagtgaaccg ttcataagct gtgcgaaagt aatgacaatg tccgatgact
    14641 ttacaacact gttagcgttc attggaggag atactgtatt gataaattga ttaattggat
    14701 ttgactttag ggaaatcgaa aaggcgaaag taaaaataaa gattaaaatt aatttgtttg
    14761 ggattttcat atatactccg ttattttttt agtttaaaat taaaaagtta aattattaat
    14821 taaggactta ttgtcattac taaatttgaa gaattatttt caacaatagc tgcatcagac
    14881 agatcgacgg taaaatctcc gttaacatcc gagttaacat agccagtaac ggagaaaaaa
    14941 atgtcattat caactattac ttcatctgaa aaatctatta aattatcatt atccaaatct
    15001 cctgaaaaaa tacaatattc gctccccaca tttaccagat ttcctccata agcagcggaa
    15061 tatccggtag taaaatcata attagcatca acgccgttag aaattgaaat tgtatttgca
    15121 ctccatgttt caagagaatt tcttccaaat acgacaacat aataattccc cggcgataca
    15181 tgcggaaaaa taaaattacc cagaaatgtt acagagtcaa ttacggattt tacttcggaa
    15241 gctattccat aaggagaagc cgaatttctt aactgcgcgg ataaagtgtc gcgtaaatta
    15301 tgctttccgg ttccggtatt taaaaagcct tctaccgcac aatttatatt tacaactgaa
    15361 ggaggtctcc attcatttaa tttatcaatg ctcattttag aaagataaat actatagtat
    15421 ttaccattaa tttcagaaaa ttgtctagtg ctatatacgc agttttgggt aaaataaaag
    15481 gaatgaagaa ttgtactttc tgctgaataa aaataaacag aatctcctac tatcgtacca
    15541 acagaataaa ttgtattaat agaatcgtgt aaagccataa aaggttgtct gttatttgtt
    15601 ctgtgccaat cttcaccatt atcagaccac attaattcaa catctgatgt atattcagga
    15661 tgagtatgag gccaattatt attttccccg ccattatttc caactctata cattccaact
    15721 aagccccacc agtcttcccc gtttctgaaa acctgcatgt aatatgtttg agtacgataa
    15781 tcaggtgagg ctggattgat atattctaga gtgtcaattt caactattac atttggatta
    15841 ctccaattta aagtatttgg gaaataaata ctgtttttca ttaaagctat ttttcgaact
    15901 ccatcaccaa acgaaacaca ccctgatgtg aaacaacatg aaggttcaat gttataaggt
    15961 ctcacatatc cccagtattc attagtgtct tcattccaaa taaaagattt atcttctcca
    16021 agttccatta cttttgtttt atttgaaaaa ttaaatcccc catcagttga aaccatagaa
    16081 taaaaaaatg atctatttgt accacttaca attctccagt cattatatcc cataaacatt
    16141 ttcatagaat ctggatttaa tactatcgat tcatacatac tgtccgtaaa tactctttgc
    16201 gtatcagtcc atgtaatacc gtaatcagtt gatcgagcaa gatatctttt atcccaggaa
    16261 ttgccattga gataatacat tttaaaagta tcttgccctg cattatcctc atatatcata
    16321 ggcatataaa gaaaaaataa tctgttacca tcgctgttat gcggaattac ggctgttgga
    16381 tatctgacgg gataagtaag ctgactgaaa gaatttgtag ggaagatgaa acttaagcaa
    16441 gcaagcaagc aagcaagcaa gcaagcattt ttaaaggcag taatatgatt cttcataact
    16501 tctcctttta ttatttattg gaacttatgt ataaggatta ttgaaaacaa gacttatttt
    16561 tttgctgacg gaatttttac aaaccgaacc atgtaagtta ctaagcaaaa aaaaaaatta
    16621 ttttttgtcg tggggaaagt aaattcttga cttatttgtt tatagacgct tctctcactt
    16681 agagcaacaa cggcaaacaa agataaattt tttccccggt ttttggagat aaatgtcata
    16741 gtgttttccg tttgcggaca ttcatttatt ttcaaaaccg gatacataat actaaattgt
    16801 attttcataa tacaagaatt atattgacag aacgaaattg ttcttagtaa tctgacttat
    16861 ggagaaaggt ggagggatta ggccctatga taccttggca accg
//
LOCUS       DAORVQ010000051        16757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1465_length_16757_cov_4.961083, whole genome shotgun sequence.
ACCESSION   DAORVQ010000051 DAORVQ010000000
VERSION     DAORVQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16757
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_16757_cov_4.961083"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLX80_05985"
     CDS             <1..314
                     /locus_tag="PLX80_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQY20380.1"
                     /translation="DDAIIKKDFYCLHHPEGITEGYSYNCECRKPKTFFVDKAVGNYN
                     IDRNASWFIGDRETDIECGINAGLKTVLIKNKYNNYTDLPKPDFISVDLKSAVKIILN
                     K"
     gene            322..1038
                     /locus_tag="PLX80_05990"
     CDS             322..1038
                     /locus_tag="PLX80_05990"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HQY20381.1"
                     /translation="MNSSKDLKIKIFSDGANIDEMKRVYSEGIVSGFTTNPTLMKKDG
                     VTDYVAFAKNVLAEIKDMPISFEVFTDDFESMERQANEIASWGENVYIKIPVTNTKDK
                     PSYELINKLSSKGFKLNVTAILTIDQVRKVYESLDKNTPSIISVFAGRIADTGRDPIP
                     YMKESYEIVKSSEKVELLWASSRELLNIIQADECGCHIITVTNDLLKKLKMVGKDLKE
                     LSLDTVKMFYNDAVSAGYDF"
     gene            1074..2459
                     /locus_tag="PLX80_05995"
     CDS             1074..2459
                     /locus_tag="PLX80_05995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQY20382.1"
                     /translation="MTKRKEKIFLFLSDLIFISLAWTVYYYIRVDTGWINYTNPPAFI
                     APLIAIYFYWLIIFSFAGLYQHWFVRSRFDEFSSVFKTVSVGCFILFFLIYLDDMMND
                     APIISRFLILIYWILTVVFISTGRIIIRSIQRNLLEKGIGLRETLIVGRGEKASELKN
                     MIIKYPQLGYRFAGYISIEDPDTGNENSGIAGLNEIKNIIAEKEISEILIALELNEKD
                     KLIDVIRYCSEEKVNLKILPDMYEIVSGMARTNQIYGVPLIEVMPDIMSPAGLLTKRI
                     IDVIISGITLIFLSPLLLIVGIFVKLTSDGPVFYKQERVGKKGLIFVMYKFRSMVVNS
                     EEYGPEWAGDNDPRITNFGKLLRKLYIDEIPQMFNVLKNEMSLIGPRPERPFFVEQLK
                     KEIPYYYKRLSVKPGITGWAQIKHKYDTSVDDVKSKIQFDFYYIENMSLKLDFKIMIN
                     TIIVILLMKGH"
     gene            2462..3184
                     /locus_tag="PLX80_06000"
     CDS             2462..3184
                     /locus_tag="PLX80_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQY20383.1"
                     /translation="MKRILVIIPTYNEADNILKIIPLILENSDQDNEYNILVVDDNSP
                     DGTAKLVEDLNNNHVNILKREKKLGLGTAYVAGFRYAIKEKYDCVFEMDADFSHDPKY
                     LKGFIEKIDEGYDLVIGSRYIDGISVLNWPLRRLILSYLASVYTRAVTGLKVMDTTAG
                     FMCYKVDSLKQIDLDNVRSNGYSFQIEMKFKMYKKGFKIIEVPILFIDRREGESKMSR
                     KVVYEAYFMVWKLKFKSVFGKL"
     gene            3246..5198
                     /locus_tag="PLX80_06005"
     CDS             3246..5198
                     /locus_tag="PLX80_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20384.1"
                     /translation="MNSKAFDISVIIVNYNVKELVDNCIASIYTANNNEYSLEIFLVD
                     NNSIDESPEHIEKKYPDVNVIRNSKNIGFSKANNIAIKKALGKYILILNPDTLLEENT
                     FNKLIKFCDEHKHTGAVTSKLILGNGKLDSACKRSFPIPSVAIPRILGLSSLFPQSKI
                     FGKYNLTYLDEDKTWEVDAICGAFMFIPKEVLEKTGYFDEDYFMYGEDIDLCFRIKKH
                     GFKIYYFPEVTTIHFKGESTRKTNLSYVNNFYGAMIIFVKKNFTGFSRSLSPLLQFGI
                     FWRSLFSYLQRIIRLFIFPISDILLLFLSLIISVKIRFDIFPNKDYMFIISIYVLIWL
                     VTLAIFGSYSKKHFLSLRNTFNAVIVGFFINSSITYFFKEYAFSRSVILASTVLSIFF
                     LLIFRGVYKLYLFFVSKNILLNKVNLLQVGSEKLSQNVEDKLNSKYNIFHYSDVSKKN
                     NISELEEIITINKIDEVVFAGNLFSNQDMLNLMWDMRNRNVRFRIFPSGKELMLSKLN
                     TSSIDEINLIEIEYNINNKLNIFLKRSFDIIFSVILLISVYPVLIILMKLFRYKPSKH
                     FSKLLLLPDVFYGNTSFVGYPVWLDNNKKQYIGKKGLTGLVQLNYYKGVSSDEILNMN
                     LFYAKNQSLILDIEILLKTFILTFKK"
     gene            5201..6154
                     /locus_tag="PLX80_06010"
     CDS             5201..6154
                     /locus_tag="PLX80_06010"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQY20385.1"
                     /translation="MAVQNYLEIEKPIITLENKIEEMRKMSDQLDISHEISKLEERIN
                     LLRKEIFSNLTPWQIVQLARHPERPFALDYIYLMTDNFVELHGDRYFGDDKAIVGGFA
                     KLDDRTVMIIGQQKGRDTKSNLYRNFGMPNPEGYRKALRLMKLAEKFNKPVITLIDTP
                     GAYPGIEAEERGQAEAIARNLYEMSGLKVPIIVAIIGEGASGGALGIGIGDRILMLKY
                     CWYSVIAPESCSQILWRSWDFKEQAAESLKLTAKDMLELKIIDRIVEEPEGGAHRDHV
                     QAAEFLKKALIEELDVVSKIKKDKFLDNRIKKFGEMGFFKD"
     gene            6203..7519
                     /gene="murA"
                     /locus_tag="PLX80_06015"
     CDS             6203..7519
                     /gene="murA"
                     /locus_tag="PLX80_06015"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQY20386.1"
                     /translation="MSGINTKFLIKGGRPLHGKVKVSGAKNSATKLLVASLLTEEECI
                     LHNSPNKISDLTITTEICSSLGSKIEFEDDILRSKSEKIETSHISEELGNKNRIAILL
                     AGPLLLRTGKAEIPIPGGCKIGSRPVNFHIDSLEKLGCNVTVKDNYFILQADYLKGNN
                     IRLDYSSVGATENIIIASTLAKGRTVITNAAVEPEIIDLIKFLQKMGAIIEINTDRKI
                     TIEGVDKLTGAEHYVIPDRNQSASFACAGLASKGDIFIENAVQENLITFLNTIRRVGG
                     KFEVKEKGIRFYYDGEMNSIPIETNVHPGFMTDWQQPFVILMTQAKGISIVHETVYEE
                     RFGYVSELQKMGAEIELYDTCLGGLQCRFANTHYFHSAVIKGPTPLHSAEINVPDLRA
                     GFSYLIAGVIAKGESTVNGAVYIDRGYEEIDKKLSDLGADIKRIED"
     gene            7582..9123
                     /locus_tag="PLX80_06020"
     CDS             7582..9123
                     /locus_tag="PLX80_06020"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQY20387.1"
                     /translation="MKNKIDILKEKRNEALAGGGQSRIDSQHKKGKLTARERIDLLLD
                     EGSFVETDIFVRHQSNDFDLAKQRFYTDGVVTGTGKIEGRTICVFSQDFTIHGGSLAE
                     YHAKKICKIMDLAMKIGVPVIGINDSGGARIQEGVVSLGGYADIFLRNTLASGVVPQI
                     SAIVGPCAGGAVYSPAITDFIFMVDKVSYMFVTGPNVVKTVTHEEVSFDDLGGAHTHA
                     VKSGVSHFTAENEIECFQNIRKLVSYVPLNNMDPVPLREYNGGEKNLEFLNTFMPDNP
                     NKPYDVKDVIKALIDEESFFEVHAEYAENIVVGFGRINGRSIGIVANQPLVLAGVLDL
                     NSSIKAARFVRFCDAFNIPLLVLEDVPGFLPGTDQEWRGVIRHGAKLLYAFCEATVPK
                     ITVILRKAYGGAYDVMNSKHIRGDFNLAWPTAEIAVMGAQGAAEIIFKKEIDAAEDKV
                     AALKEKVDEFTEKFANPYLAAERGFVDDVIEPKDTREKLIYYFEILENKVDSNPKKKH
                     GNIPL"
     gene            complement(9203..10582)
                     /locus_tag="PLX80_06025"
     CDS             complement(9203..10582)
                     /locus_tag="PLX80_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQY20388.1"
                     /translation="MKFKTIFEPFKIKSVEPIKLTSEEERNRLLEEADYNPFQLLSKN
                     VLIDLLTDSGTSAMSSKQWSAIMDGDEAYAGSKSWIKMEKRIKDLTGFEYILPTHQGR
                     AGERILYGYLGGKGKIFISNTHFDTTRANIEYSGAAAIDIPIAESNEPMLSHPFKGNM
                     DTEKLKNLLKEYGSENIGGVILTVTNNSGGGQPVSMKNANEVSNLCRKFNVKFFLDCC
                     RIAENSYFIRSREPGYNRISYKNIAKEMFALSDGSIMSAKKDGLVNMGGFLALKDKEL
                     YEACTNLLIITEGFVTYGGLTGRDMEAIAIGLKEVFDKDYLKYRIRSTEYLGEMLYKM
                     GVPLIWPIGGHAVYIDAKSFYPHIPVEQYPGQALVCELYLKGGIRSVEIGSVMFGKYD
                     KKGRLIPAPNELVRLAIPRRVYTQSHIEYVIEVFKMLIENKDSVKGMVITKEPEFLRH
                     FTAKFKYAE"
     gene            10682..11791
                     /gene="egtD"
                     /locus_tag="PLX80_06030"
     CDS             10682..11791
                     /gene="egtD"
                     /locus_tag="PLX80_06030"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HQY20389.1"
                     /translation="MKAQIPKPLSTIYMPNYLYLKIELNPIDIKKSENILKKFNYHKY
                     NERLEIFTSLKLKKGKTFAEEVKNGLSAKNKSLPPKYFYDKTGSLLFEKICKTKEYYP
                     TVTEKKILIAKAEEISKKNMKINLVTELGSGSSEKSAYLIKAFLNERNSLHYIPIDVS
                     EILYESSLNLVKKYKKLSVSGIISFYENGIKLIKSIDDSPKLILFLGSSIGNFTRKES
                     VKFLTNVSRFMNPDDRLLVGFDLIKDENVLNNAYNDKDGYTKKFNLNILKRINRELHA
                     NFPLSKFSHKAFYNSNKKRIEMHLIANDDLTVEIGDDKYKINFTKGESIHTENSCKFN
                     FDMIHVLADQSGMKISDQYTDNKNFFTITSLTLKT"
     gene            11973..13034
                     /gene="pstS"
                     /locus_tag="PLX80_06035"
     CDS             11973..13034
                     /gene="pstS"
                     /locus_tag="PLX80_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HQY20390.1"
                     /translation="MYKVISIFILTLVLFSCGKKDVSMDKPGDKSGGQINGAGATFPY
                     PIYSKWFNEYAKIDKDAKINYQSIGSGGGIKQITEGTVDFGATDSPMSDEEIEKANIK
                     NNNKIIHIPTVIGSVVITYNLPGVENNLKLTGEIISGIYMGKIKKWNDAKIKVENPGV
                     NLPDKEIIVCSRTDGSGTTFVFTDYLGKVDPDWKTNIGVAKDVKFPVGQGGKGNEGVT
                     GLVKQLEYSIGYVELVYALQNNLKYAEIKNADGEFILPTLETLTNAADNSISDMPDDL
                     RISITNAKGKNSYPISSFTYILIFDTQKNAFNGKTMKAFLEWALTTGESYATELGYAP
                     LPNSVTEKALTKIKGIKVQ"
     gene            13112..13996
                     /gene="pstC"
                     /locus_tag="PLX80_06040"
     CDS             13112..13996
                     /gene="pstC"
                     /locus_tag="PLX80_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQY20391.1"
                     /translation="MFFENLTLIFALSILGLVIYMIYEMFIDSSESIEKFGFGFIFSE
                     NWDPVKEEFGALTFIYGTIVSSFLALLISLPISIGVAIFLSEIVHKSIKTSLSFLIEI
                     LAAIPSIIYGFWGIFVLAPYMRTTIQPFLQDVFGFLPFFQGTMYGTGLLTASIILAIM
                     ITPIITAITRDVLKAIPVSQKEAAYALGATRWEGIKMALLNARSGILGGAVLGLGRAI
                     GETMAVTMVIGNKAQIKASLFEPAYTMASVIANEFAEASGGMQVSALIEVGLILFGVT
                     FIVNSMARLLIYSVTRQS"
     gene            14016..14879
                     /gene="pstA"
                     /locus_tag="PLX80_06045"
     CDS             14016..14879
                     /gene="pstA"
                     /locus_tag="PLX80_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQY20392.1"
                     /translation="MTADKIFKNPKNLKRRKFVNSLMIFLCVVASVLTILPLLYIFIY
                     TTQSGFSSLNIDFFTQLPKPVGEEGGGMANAIVGSGILLGIGGLIGIPIGILSGIYIA
                     EYSKSFLASAVKFTTDVLSGVPSIVVGIFAYGVIVLTMQSFSAIAGGFALGILMIPTI
                     TKITEEMLKLVPQSLREASLALGVSRWKTTLKVVLKTASSGIVTGVLLAIARAAGETA
                     PLLFTAFGNAFWQTNIDQPIAALPLQIFVYAISPYEDWHRQAWAGAFVLIFLVFIVNF
                     LVRFVTREKYS"
     gene            14894..15751
                     /gene="pstB"
                     /locus_tag="PLX80_06050"
     CDS             14894..15751
                     /gene="pstB"
                     /locus_tag="PLX80_06050"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQY20393.1"
                     /translation="MDIIPKKDEDFKEAKVLVSELEKEEDGDDGTGNIKTKSAVLTTD
                     LSVYFNKRPAIENINIKIKKNSVTAIIGPSGCGKSTFLRSLNMMHDMTPKAQVFGEIY
                     IHGENIVNFNTVELRKRVGMVFQKPNPFPTMTIYDNVAAGFKLNGIGKKSELDEIVEK
                     NLVNAGLWEEVKDRLKSPALSLSGGQQQRLCIARTIAVNPEIILMDEPTSALDPVSSS
                     KIEELIYNLKKEFTIVIVTHNMQQAARVSDQTAFFYLGKLVEISKTRKMFTNPKEKLT
                     EEYVTGKFG"
     gene            15868..>16757
                     /locus_tag="PLX80_06055"
     CDS             15868..>16757
                     /locus_tag="PLX80_06055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQY20394.1"
                     /translation="MNHYTKIYSVVLPAAILFFSVSGFIGSKSGKNNSFIKTDFITES
                     GGLKIYNAGNVNILSPDDLSSGLKDTVYTDKECWLELKIEKQMLYQHWRNGTVTSYPV
                     STGNNKGGDPEALESRPGLFAIFLKVEHHQSSQFNSSNMYNFMPFNQGIGFHSIDGTS
                     YYAHLGVRPSSHGCIRMKHDDVKKLFKECPLGTLVLASKGYTARTVAFAPEGFVNKKE
                     YSKDDYKLMLAENLYNIINGKYYMQEREMFVVNPKVIPVSGVYTSYDVKIPQKQIIPR
                     THLTVTELKDNLNIIESKSI"
BASE COUNT         5826 a         2454 c         3101 g         5376 t
ORIGIN      
        1 aagatgatgc tattataaaa aaagacttct attgtttaca tcatccggaa ggaattactg
       61 aaggttattc ttataattgt gaatgcagaa agcctaaaac attctttgta gataaggcag
      121 tcgggaatta taatattgac agaaatgcat catggtttat cggtgacaga gagacagaca
      181 tagaatgtgg tattaatgct gggttaaaga cggttctaat aaaaaataaa tataataatt
      241 atacagattt accaaagccg gattttattt cggttgatct gaaatctgca gtaaaaataa
      301 ttttgaataa ataaaaaata aatgaacagt tcaaaagatc ttaaaataaa gatattttct
      361 gacggagcaa atatagatga aatgaaaaga gtttacagtg aaggtatagt ttccggattt
      421 accactaatc ctaccttgat gaaaaaagat ggagttactg attatgtggc ttttgcaaaa
      481 aatgtattgg cagagataaa agatatgcca atatcatttg aagtgtttac agatgatttt
      541 gaatctatgg aaagacaggc aaatgaaata gcaagctggg gagagaacgt gtatataaaa
      601 attcctgtaa caaatacgaa agataaacca tcatatgagc tgataaataa attatcatca
      661 aaaggtttta agctgaatgt aactgctata cttacaatag atcaggtgag aaaagtatat
      721 gagagtcttg ataaaaatac accttctatt atttcagtct ttgcaggaag gatcgcagat
      781 acaggcagag atccgatccc ttatatgaaa gagagttatg agattgtaaa aagttctgag
      841 aaagttgaat tactttgggc gagttcaaga gagttactga atataataca ggcagatgaa
      901 tgtggatgtc atattattac agttacaaat gatcttttga aaaaactgaa aatggttggt
      961 aaagatctga aagagctttc gcttgatacg gtaaaaatgt tttacaatga tgcagtatct
     1021 gcaggttatg atttttaatc atagaaaata atttagaata ttagtcagaa aatttgacca
     1081 agaggaagga aaaaatattt ttatttctgt ccgatctgat ctttatttca ttggcatgga
     1141 cagtttatta ttatatcagg gttgataccg gttggatcaa ttatacaaac ccgccggcat
     1201 ttatcgcgcc gcttattgca atttattttt actggttgat aattttttca tttgctggat
     1261 tatatcagca ctggtttgta agatcaagat ttgatgaatt ttcttctgta tttaaaacag
     1321 tttcagtagg ttgtttcata ttgttctttc ttatatatct ggatgacatg atgaatgatg
     1381 caccgatcat ttccagattt cttattctga tatactggat tcttacggtt gtcttcatta
     1441 gtacaggcag gatcattatc agaagtattc aaaggaattt acttgaaaaa ggaattggtc
     1501 tcagggagac attaattgtc ggcagaggtg aaaaagcttc tgagctgaaa aacatgatca
     1561 taaaatatcc tcagcttgga tacagatttg ccgggtatat tagtatagaa gatccagaca
     1621 caggtaatga aaacagcggt attgcaggac ttaatgaaat taaaaatata attgctgaaa
     1681 aggaaatatc tgaaattctt attgctcttg aattgaatga aaaggataaa cttattgatg
     1741 taataagata ctgcagtgaa gaaaaagtta atttaaaaat tttacctgac atgtatgaga
     1801 tagtcagcgg aatggcaaga acaaatcaga tttatggagt accgctgatt gaagtaatgc
     1861 ctgatattat gtctccggcc ggattactta caaaaagaat tattgatgta ataatttcag
     1921 gtataacttt gatatttctt tcaccgttac ttttgatcgt aggaattttt gtaaaactaa
     1981 cttctgacgg accggtgttt tataaacaag aaagagtcgg aaaaaaggga ttgatatttg
     2041 ttatgtataa gttcagatca atggttgtaa actccgaaga atatggtcct gaatgggcag
     2101 gtgataatga tccgaggatt acaaattttg gaaaactgtt gaggaaatta tatatagatg
     2161 aaatacctca gatgtttaat gttttgaaaa acgaaatgag tcttattggt ccaagacctg
     2221 agagaccttt ttttgtagag caattgaaaa aagaaatacc ttattattat aaaagattat
     2281 ctgtaaaacc cggtattacc ggctgggcac agataaaaca taaatatgac acttctgtgg
     2341 atgatgtaaa atcgaaaatt caatttgatt tttattacat agaaaacatg agtttaaaat
     2401 tagatttcaa aataatgata aatactatta ttgtaatttt attaatgaaa ggtcactgaa
     2461 tttgaaaaga atattagtga tcattcctac atataatgaa gctgataata tattaaagat
     2521 cattccgttg attcttgaaa acagtgatca ggataatgaa tacaatatac ttgtagtcga
     2581 tgataattca ccggacggaa ctgcaaaact tgttgaagat cttaacaaca atcatgtaaa
     2641 tattttaaag cgtgaaaaaa aacttggtct tggaactgct tatgtcgcag gattcagata
     2701 tgcaataaaa gaaaagtatg actgcgtctt tgaaatggat gctgattttt ctcatgatcc
     2761 aaaatatctt aaaggtttta ttgagaagat cgatgaaggt tatgatcttg tgatcggatc
     2821 aagatacatt gacggaattt ctgttctgaa ctggcctctt agaagactta tccttagcta
     2881 tctcgcatca gtttatacaa gagcagttac aggtctgaaa gtaatggata caacggcagg
     2941 atttatgtgc tacaaagttg attcgctgaa gcagatagat cttgataatg taagatcaaa
     3001 cggatattca tttcagatcg agatgaaatt taagatgtat aaaaaaggat ttaagataat
     3061 cgaagttcct attctattta ttgacagaag ggaaggtgaa tccaagatgt cccgcaaagt
     3121 agtatatgaa gcatatttca tggtctggaa attaaaattc aaatcggtat tcgggaagtt
     3181 ataatttttt tatgtaaatg aatttcagaa ttttatgaaa tttcctgtat atcaaaaatc
     3241 attaaatgaa ctccaaagca ttcgacattt cggttataat tgtaaactac aatgtaaagg
     3301 aacttgtcga taactgcata gcctcaattt acaccgcaaa caataacgaa tacagtctcg
     3361 aaattttttt agttgataat aattcgatag atgaaagtcc ggagcatata gaaaaaaaat
     3421 atcctgatgt taatgtgatc aggaattcaa agaacattgg tttttccaaa gccaacaata
     3481 ttgcaataaa gaaagctttg ggaaaatata ttttaattct aaatcctgat acactccttg
     3541 aagaaaatac atttaataag cttataaaat tttgtgatga acataagcat accggtgctg
     3601 tcacatcaaa gctgatcctc ggtaacggta aacttgactc agcatgtaaa agaagttttc
     3661 cgataccatc tgttgcaata ccaaggatac ttggtctatc atcattattt cctcaaagca
     3721 aaatattcgg aaaatataat ctcacatatc tcgacgaaga taaaacatgg gaagtagatg
     3781 ctatctgcgg tgcgtttatg tttattccaa aagaggtttt ggaaaaaaca ggatactttg
     3841 atgaagatta ttttatgtac ggtgaagata ttgatctttg cttcagaata aagaaacacg
     3901 gatttaaaat ttattatttt cctgaagtta cgacaataca ttttaaagga gagagtacac
     3961 gcaagactaa tttatcttat gtgaataatt tctatggtgc catgataatt tttgtaaaaa
     4021 aaaatttcac aggattttca agatcattgt caccgcttct tcagtttgga atcttctgga
     4081 gatctttgtt ttcatatctg cagaggatca tcagattatt tatattcccg atctcagata
     4141 ttcttctttt atttctatcg ctgataattt ctgtaaagat cagatttgat atttttccga
     4201 ataaagatta catgttcatc atttcaatat acgtattgat atggcttgtg actcttgcaa
     4261 tattcggatc atattccaaa aagcatttcc tttcacttcg aaataccttc aacgcagtga
     4321 tcgtcggatt ttttataaac tcttcaataa catatttctt taaagaatat gctttttcaa
     4381 gaagtgtgat acttgcatca actgtattgt caattttttt cctgctgata ttccggggcg
     4441 tatataaact gtacttattt tttgtatcga aaaacattct cctaaataaa gtaaatcttc
     4501 tgcaggtagg cagcgaaaag cttagtcaga atgtagaaga taaattgaat tcaaaatata
     4561 atatatttca ttattcagat gtttccaaaa aaaataatat ttcagaactg gaagaaatta
     4621 tcactataaa taaaattgac gaagtagtat ttgcaggtaa tcttttttct aatcaggaca
     4681 tgctcaatct tatgtgggat atgcggaaca gaaatgtcag gttcaggatt ttcccctcag
     4741 gcaaagagct tatgctgtca aaattaaata ccagcagtat tgatgagatt aacctaatag
     4801 aaattgaata taatattaat aataaattaa atatattttt aaaaagatct tttgacataa
     4861 tattctcagt aattcttctc atttcggttt accccgtttt gataatcctg atgaagttat
     4921 tcagatataa accttcgaaa catttctcaa agcttttatt gcttccggat gttttttatg
     4981 gaaataccag ttttgtaggt tatccggtat ggctggacaa taacaagaaa caatatatag
     5041 gtaagaaagg acttaccgga cttgttcagt tgaattatta taaaggagtt tcctcagatg
     5101 aaatactcaa catgaattta ttttatgcaa aaaatcagtc tttgatactt gatattgaga
     5161 tactactaaa aacttttata ttaacattta aaaaatagtt atggcagttc aaaattattt
     5221 agaaattgag aaaccaataa ttacacttga aaataagatc gaggagatga gaaagatgtc
     5281 agatcaactt gatatttcac atgaaatcag taaactggaa gagagaataa atttattaag
     5341 aaaagagata ttcagtaatc tgactccatg gcagatcgtt cagcttgcaa gacatcctga
     5401 aagaccattt gctttggatt atatttattt gatgactgat aattttgttg agctccacgg
     5461 tgacagatat tttggtgatg ataaagctat agttggtgga tttgcgaaac ttgatgacag
     5521 aactgtaatg ataattggtc agcaaaaagg acgtgataca aaatcaaatc tttacagaaa
     5581 ttttggcatg cccaatccag aaggttacag aaaagcttta agattaatga agcttgctga
     5641 aaaatttaat aaacctgtaa ttactttgat cgatactccg ggtgcatatc cgggtatcga
     5701 agctgaggaa aggggacagg cagaagctat tgcaagaaat ctttatgaga tgtcaggtct
     5761 gaaggttccg ataattgttg caattatcgg agaaggtgcc tcaggcggag cattaggaat
     5821 aggtattggt gacaggattt taatgttaaa atactgctgg tattccgtaa ttgctcctga
     5881 gtcatgttct caaattttat ggaggagctg ggattttaaa gaacaggctg ctgaatcatt
     5941 aaagctcact gcaaaagata tgctcgagct aaaaataatt gacagaatag tagaagagcc
     6001 tgaaggcgga gctcaccgag atcatgtgca ggctgctgaa tttttaaaaa aagcattgat
     6061 tgaagaatta gatgtagtaa gtaagatcaa aaaagataaa tttcttgata acaggataaa
     6121 gaaattcggt gagatgggat ttttcaaaga ctgatcaaat tcagcaaatt atattcaatt
     6181 gaaataaatt taatagaaaa aattgtccgg aataaataca aaatttttaa taaaaggcgg
     6241 aagaccatta catggaaaag tgaaagtcag cggagcaaaa aattcagcta caaaattatt
     6301 agttgcatca ctccttactg aagaagaatg tatccttcac aattcaccaa ataaaataag
     6361 tgaccttacc attacaacgg aaatatgttc ttcacttgga agtaaaatag aattcgaaga
     6421 tgatatttta agatcaaaat cggaaaaaat tgaaacttca catatttctg aagagctcgg
     6481 aaataaaaac agaatagcaa ttttacttgc aggaccttta ttgctcagaa caggaaaagc
     6541 tgagatacct atcccaggtg gatgcaagat cggttcacga cctgtaaatt tccatataga
     6601 ttcacttgag aaacttggct gtaatgtaac tgtaaaagat aattatttta tactgcaggc
     6661 tgattatctc aaaggtaaca acatcaggct ggattattca agtgtaggtg caacagaaaa
     6721 tataataatt gccagcactc ttgcaaaggg aagaactgta attactaatg cagcagtaga
     6781 gcctgagatt attgatctga taaaatttct tcaaaaaatg ggagcaatta ttgagattaa
     6841 tactgacaga aaaattacta tagaaggtgt agataaatta accggtgccg aacattatgt
     6901 tattcctgac agaaatcaat cagcttcttt tgcatgtgca ggactcgctt caaaaggtga
     6961 tatttttatt gagaatgcag tgcaggaaaa tctgatcact tttcttaata ctataagaag
     7021 ggttggcgga aaatttgaag taaaagaaaa aggaatcaga ttttattatg acggggaaat
     7081 gaattcaata cctattgaaa caaatgtgca tccgggattt atgactgact ggcagcagcc
     7141 gtttgtaatt ttgatgactc aggcaaaagg tatttcaata gttcatgaaa ctgtatatga
     7201 agaaagattt ggttatgtca gcgaactgca gaaaatgggt gcagaaatag aattatacga
     7261 tacttgtctt ggcggacttc aatgcagatt tgcgaatact cattattttc acagtgcagt
     7321 aattaaagga cctactccgc ttcactcggc tgaaattaat gtacctgatc ttagggccgg
     7381 attttcttat ttaattgccg gagtaattgc aaaaggggaa tcgacagtaa atggtgctgt
     7441 ctatattgac agaggctatg aagaaattga taaaaaactc agcgatcttg gagcagatat
     7501 taagagaatt gaagattaac atataatttt tttaaaaaaa taattttgat taaatacttt
     7561 taaactgatt tgatttaacc aatgaaaaat aaaattgata tattaaaaga aaaaaggaat
     7621 gaggcattag caggcggtgg tcaatcgaga attgactcac agcataagaa ggggaagctg
     7681 actgcaagag aaagaattga tctcttattg gatgaaggta gttttgtaga aacagacatt
     7741 tttgtaagac atcagtcaaa tgattttgat ctcgctaaac aaagatttta tactgacgga
     7801 gtcgtgacag gaacaggtaa aattgaaggc agaactatat gtgtcttctc acaggatttt
     7861 acaattcacg gaggatcact tgccgaatat catgcaaaaa aaatatgtaa gatcatggat
     7921 cttgcaatga agattggcgt gccggtaatc ggaattaatg acagcggagg tgcgagaatt
     7981 caggaaggtg tagtttctct tggcggctat gcggatattt ttttaaggaa tacacttgca
     8041 agcggagtag tacctcagat atctgcaatt gtcggtcctt gtgcaggcgg tgcagtttat
     8101 tcgcctgcaa ttacagattt tatttttatg gttgacaaag tttcatatat gtttgtcaca
     8161 ggaccaaacg tagtaaaaac agtaacgcat gaagaagtct cttttgatga tctgggaggt
     8221 gctcatactc atgctgtgaa aagcggagtc agtcacttta ctgctgaaaa tgaaattgag
     8281 tgttttcaga atatcaggaa attagtttca tatgttcctc tgaataatat ggatcctgta
     8341 cctttaagag aatataacgg tggagagaaa aacctggaat ttttgaatac ctttatgccc
     8401 gataatccca ataaacctta tgatgtcaaa gatgtaatca aggctttgat agatgaagaa
     8461 tcattttttg aagttcatgc agaatatgct gaaaatattg tagtgggatt tggaaggata
     8521 aacggaagat caataggcat tgtagcaaat cagccgctcg tactcgcagg tgtacttgat
     8581 cttaattcat ctattaaagc agcaaggttt gtaagattct gcgatgcatt taatattcct
     8641 ttacttgtac ttgaagacgt gccgggattt ttgccgggga ctgatcagga atggcgtgga
     8701 gtgatcagac acggtgcaaa acttttatat gcattctgcg aagctactgt tcccaaaatt
     8761 actgtgatac ttagaaaagc ttacggtgga gcttatgatg ttatgaattc caaacatatc
     8821 agaggcgatt ttaatcttgc ttggccgact gcagagatag cagtaatggg tgctcagggt
     8881 gctgctgaaa ttattttcaa aaaagaaatt gatgctgctg aagataaagt agcagcatta
     8941 aaagaaaagg tagacgagtt tacagaaaag tttgccaatc catatcttgc tgctgaaaga
     9001 ggtttcgtcg atgatgtgat tgaacctaaa gatacacgtg aaaaattgat ttactatttc
     9061 gaaattcttg aaaataaagt cgattcaaat cctaagaaaa agcacggaaa tattccttta
     9121 taaattgtaa aaactaaagt tcccctttga tattaaagca tccggaagtt aaatatcgga
     9181 tgattttgtt ttaagttcat attcattctg catacttaaa ctttgcagta aaatgtctga
     9241 ggaattcagg ctctttggta atgaccattc cttttactga atccttattt tcaatcagca
     9301 ttttaaaaac ttctataaca tattcgatat gactttgagt atatactctt cggggtattg
     9361 ctaaccttac aagttcattc ggtgcaggaa ttagtctccc ttttttatca tatttaccaa
     9421 acataacaga acctatctcc accgaccgga tacctccttt taaatataat tcacaaacca
     9481 aagcctgccc cggatattgc tcaacgggaa tatgtggata aaaagatttt gcatcgatat
     9541 aaaccgcatg tcctccaatt ggccagatca aaggcactcc cattttgtaa agcatttcac
     9601 caagatattc agtacttctg atcctgtatt tcagataatc tttgtcaaaa acttctttaa
     9661 gaccaatcgc tatcgcctcc atgtctcttc ctgtcagtcc gccatatgtt acaaatcctt
     9721 cggttataat taacaggttt gtacacgctt catataattc tttatctttt aatgccagaa
     9781 acccacccat atttacaagt ccgtctttct tggcactcat gatactaccg tcagaaagtg
     9841 cgaacatttc tttagctata tttttgtatg aaatcctgtt ataaccgggc tcacgggatc
     9901 tgatgaagta agaattttca gctatcctgc agcagtcgag aaaaaatttc acattaaatt
     9961 ttctacaaag attacttact tcatttgcat ttttcatgct gaccggctga ccgccgccgc
    10021 tgttgtttgt gacagttaaa attaccccgc ctatattttc gctgccgtat tccttcaata
    10081 aattttttag tttttctgta tccatattac ctttaaaagg gtgtgacagc attggttcat
    10141 tggattccgc tatcggaatg tcaatcgctg cagctccgga atattcaatg ttcgctcttg
    10201 tagtatcgaa atgtgtatta cttataaata tttttccttt accaccaaga tatccgtaaa
    10261 gaattctttc accggctctg ccctgatgtg tcggcagaat gtattcaaat ccggtcagat
    10321 ctttaattct cttttccatt tttatccagc tttttgatcc ggcataggct tcatcaccgt
    10381 ccattattgc agaccattgt tttgaactca ttgcagaagt tccgctgtca gtaagaagat
    10441 ctataagcac attttttgaa agaagctgga atggattgta gtctgcttct tcaagtaatc
    10501 tattcctctc ctcctccgag gtcaacttta ttggctctac agatttaatt ttgaatggtt
    10561 caaatattgt tttaaatttc atttaaatat ctccgttaaa atattaataa atttttattt
    10621 ttcttttcta atttaaatat actgcaaaat tttagttatg atttatgtca tataaaattt
    10681 tatgaaagct caaattccaa aacctttatc tactatttac atgcctaatt atttatattt
    10741 gaaaattgaa ttaaacccaa tagatatcaa aaaatcagaa aatatactta agaaattcaa
    10801 ttaccataaa tacaatgaaa gattagaaat atttacttca ttgaaactta agaagggaaa
    10861 aacttttgct gaagaagtga agaatggtct ttctgcaaaa aataaatctt taccgccaaa
    10921 atatttttat gataaaacag gctcacttct ttttgaaaaa atatgtaaga caaaagagta
    10981 ttacccaaca gttacggaaa agaaaattct tattgcaaaa gctgaagaga tctcgaaaaa
    11041 gaatatgaaa ataaatcttg ttacagaact tggcagcgga agttcagaaa aatcagctta
    11101 cttgataaag gcatttctaa atgaaagaaa tagtctgcat tatattccta ttgatgtttc
    11161 tgaaatactt tatgagagtt ctttaaattt ggttaaaaaa tataaaaaac tttctgtatc
    11221 tggaataatt tcgttttatg aaaatggtat taaacttatt aaaagtattg atgattctcc
    11281 aaagttaatt ttatttcttg gatcaagcat tggaaatttc acccgaaaag aatcagtaaa
    11341 attcttaact aatgtcagca gattcatgaa tcctgatgac agattgttgg tgggttttga
    11401 tctgataaaa gatgaaaatg ttttgaacaa tgcatacaac gataaagacg gttatacaaa
    11461 aaaattcaat ctgaatatac ttaagagaat aaacagagaa ctgcatgcta attttcctct
    11521 ttcaaaattt agtcataaag cattttataa ttcaaataaa aaaagaatcg aaatgcatct
    11581 cattgcaaat gatgatctga ctgtagaaat cggtgatgat aaatataaaa ttaatttcac
    11641 aaaaggtgag tccattcata ctgagaattc atgtaaattt aattttgata tgattcatgt
    11701 tctggctgac caatcaggaa tgaaaatttc agatcaatat acggacaata aaaatttttt
    11761 tacaattact tctttgacat taaaaactta atgtaattct gatatcatta atacaaaatt
    11821 atgcaataaa atttaggaaa acttttatca gtaatatttg aaatttgtca tttaaaatat
    11881 gaaaatgttt ttaatctgtt tctttacaat ttcttaacat taagaatatt ctattatgtc
    11941 tatttttgta tattaaaaat ttaaataata tcatgtataa agtaatttcg atttttattc
    12001 tcaccttagt tttgttttca tgcggtaaaa aagatgtttc aatggataaa cccggagata
    12061 aatccggtgg tcagattaac ggtgcaggag ctactttccc ttatccaata tattcaaaat
    12121 ggtttaatga atatgcaaaa attgataaag atgcaaagat caattaccag tcaataggaa
    12181 gcggtggtgg aatcaagcag ataactgaag gaacagttga cttcggtgca actgatagtc
    12241 caatgagtga cgaggaaatt gaaaaagcaa acataaaaaa taacaataag ataattcata
    12301 ttcctactgt tatcggatct gttgtaataa cttataatct gcctggagtt gaaaataatc
    12361 ttaagctaac aggagaaatc atttctggca tttacatggg gaagatcaaa aaatggaatg
    12421 acgcaaagat caaagtggaa aatccggggg tgaatttacc ggataaggaa attattgtct
    12481 gttccaggac agacggaagt ggtactactt ttgtatttac agattatctt ggtaaagtag
    12541 atcctgactg gaaaacaaat atcggcgtag caaaggatgt taagtttcct gtcggtcagg
    12601 gcggaaaggg taatgaagga gtcacaggac ttgtgaagca acttgaatat tcaataggtt
    12661 atgttgagct agtctatgcg cttcagaata atttaaaata tgccgagatc aaaaatgcag
    12721 atggtgaatt tattttaccg acacttgaaa cacttaccaa tgcagcagat aattcgatct
    12781 cagatatgcc tgatgatctt agaatatcta tcacaaatgc aaaaggaaaa aattcttatc
    12841 caatctcaag ttttacttat attcttattt ttgacacaca gaaaaatgca tttaatggta
    12901 aaacaatgaa agcatttctt gaatgggcgc taacaacggg tgaatcttat gcaacagaat
    12961 tgggatatgc tcctcttcca aacagtgtta ctgaaaaggc attgaccaaa ataaaaggaa
    13021 ttaaagttca gtaaatatta aaattaaatt aatataatca gctacggata attctaaaaa
    13081 tgaaataaag ggtaaaaaga aacttgggga tattttcttt gagaatctta cactcatatt
    13141 tgctctgagc atcctgggtt tagtaatata catgatatat gagatgttta ttgactcatc
    13201 cgaaagcata gaaaaatttg gtttcggatt tattttttca gagaactggg atcctgtgaa
    13261 ggaagaattc ggagcactga ctttcattta cggaacaata gtatcgtcat ttcttgcact
    13321 ccttatttca ctaccgatca gtattggagt cgcaatcttt ttatcagaga ttgtacataa
    13381 gtccataaaa acttcactga gttttttgat tgagattctt gcagctattc cgagcattat
    13441 ttacggcttt tggggaatat tcgtactggc tccatatatg cgaacgacaa ttcagccatt
    13501 ccttcaggat gtatttggat tccttccatt ttttcagggg acaatgtatg gtactggatt
    13561 attgacagct tcaataattt tagcaataat gatcacacca attattactg cgatcacaag
    13621 agatgtatta aaggcgattc ctgtttctca aaaagaagct gcatatgcat taggtgcgac
    13681 ccgatgggaa ggaataaaaa tggcattatt aaatgcacga agcggaatct taggtggtgc
    13741 tgtgcttgga ctaggtagag caatagggga gacaatggct gttacaatgg taattggaaa
    13801 taaagctcag atcaaagctt ctttatttga accggcttat acaatggcat ctgtcattgc
    13861 aaatgaattt gcagaagctt caggaggaat gcaggtcagt gctttgattg aagttgggct
    13921 gatcttattt ggcgtaacat ttatagtaaa ttcaatggca agattattaa tttacagtgt
    13981 tacaagacaa tcatgataaa cagatttata tataaatgac agcagataaa atatttaaaa
    14041 atccgaagaa tcttaaacgc aggaaatttg tcaattcact gatgatattt ctttgtgtgg
    14101 ttgcatctgt attaacgatt ctaccgcttc tttatatatt tatttacact actcaatccg
    14161 gcttttcatc tctgaatatt gattttttta ctcagcttcc aaaaccggta ggtgaagaag
    14221 gtggtggaat ggcaaatgca attgtcggca gcggaatact tttaggtatt ggcgggctta
    14281 tcggaattcc tatcggtatt cttagtggaa tttatatagc agaatattcc aaaagttttc
    14341 ttgccagtgc cgttaaattt acaaccgatg tattaagcgg tgtaccatct atagttgttg
    14401 gaatttttgc ttacggtgtg atagtactta caatgcagag ctttagtgca atagccggtg
    14461 gctttgcatt aggaatactt atgatcccga ctattacaaa gataactgaa gaaatgctta
    14521 aactcgtacc gcagtcattg cgtgaagcct cacttgcctt aggagtttcg agatggaaaa
    14581 caactttgaa ggtagtattg aaaactgctt catcaggtat tgtaacaggt gttttacttg
    14641 caatagccag agcagcaggt gagacagcgc ctttactttt tacagctttt ggaaatgctt
    14701 tctggcagac aaatatagat cagccgattg cagcactgcc gcttcagata tttgtttatg
    14761 ctatctctcc ttatgaagac tggcacagac aggcatgggc tggggcattt gtacttatat
    14821 ttcttgtatt cattgtcaac tttcttgtga gatttgtaac gcgggaaaaa tacagttaaa
    14881 ttataatatt taaatggaca taattccaaa aaaagatgaa gattttaaag aagctaaagt
    14941 gttagtttca gaattggaaa aggaagaaga tggagatgac gggactggaa atataaagac
    15001 taagtcagct gtgttgacaa ccgatttatc ggtttatttt aataagagac cggcgattga
    15061 aaatataaat atcaaaataa aaaagaattc agttactgca attattggac cttcgggctg
    15121 cggaaaatct actttcctca gatctctgaa tatgatgcat gatatgactc caaaagctca
    15181 ggtgtttgga gagatttata ttcatggaga aaatattgtt aactttaata ctgttgaatt
    15241 aagaaaaaga gtcggtatgg tttttcaaaa accaaatcct tttcctacaa tgacaattta
    15301 tgataatgta gctgcaggtt ttaaattaaa tggaattggt aagaaatctg aacttgatga
    15361 aatagtagaa aaaaatcttg taaatgcagg tctttgggaa gaagtgaagg atagactgaa
    15421 atctccggct ttaagtcttt caggtggtca gcaacagaga ttatgtatag ccagaacaat
    15481 tgctgttaat ccggagatca tacttatgga tgaaccaacg agtgcacttg atcctgtatc
    15541 atcttcgaaa attgaagaac tgatttataa tctgaaaaaa gaatttacaa ttgtaattgt
    15601 aactcataat atgcagcaag ctgctagagt aagcgaccaa actgcattct tttatctggg
    15661 aaaactggtt gagatttcca aaacccggaa aatgttcaca aacccaaaag aaaaacttac
    15721 agaagagtat gtaaccggaa aatttgggta attattaatt tgtttaaatt taatattatt
    15781 tttgatattt ttgatatcgt aatattaaat ctaacttatt taaactaact aaagatattc
    15841 cggacagatt tttttcgggt aaaaattttg aatcattata ctaaaatata ttctgtggtt
    15901 ttacctgcag ctattctatt tttttctgta agtggtttta ttggtagtaa atcaggaaaa
    15961 aataattcat ttattaaaac tgattttatt acagaatcag gcggtttaaa aatttataat
    16021 gcaggtaatg taaatatttt atcacccgat gatctttctt cgggtttaaa agatactgtt
    16081 tatactgaca aagagtgctg gttagaattg aaaattgaaa agcaaatgct ctatcagcac
    16141 tggagaaacg gtacagttac ttcttatcct gtttctactg gaaataacaa aggtggtgat
    16201 cctgaagcac ttgaatcaag acctgggttg tttgcgatat ttctaaaagt agaacatcat
    16261 cagtcttcac aattcaattc ttcaaatatg tataatttca tgccattcaa tcagggaatt
    16321 ggattccatt ctattgatgg tacaagttat tatgcgcatc ttggtgtaag accttcttct
    16381 cacggttgta tcagaatgaa acatgatgat gtgaaaaaac tatttaaaga atgtccttta
    16441 ggtactttgg tattagcaag taaaggttat actgcccgaa ctgttgcttt tgctccggaa
    16501 ggatttgtaa ataaaaaaga atattcaaaa gatgattata aattgatgct tgctgaaaat
    16561 ctgtataata ttattaatgg aaaatactat atgcaggaaa gagaaatgtt tgttgtaaac
    16621 cccaaagtaa ttcctgtttc aggtgtttat actagttatg atgtgaagat tcctcaaaag
    16681 caaattattc caagaacaca tttgactgta acagaattaa aagataacct caatataatt
    16741 gagagcaaaa gtatttt
//
LOCUS       DAORVQ010000052        16718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1473_length_16718_cov_6.042729, whole genome shotgun sequence.
ACCESSION   DAORVQ010000052 DAORVQ010000000
VERSION     DAORVQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_16718_cov_6.042729"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1813
                     /locus_tag="PLX80_06060"
     CDS             134..1813
                     /locus_tag="PLX80_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQY20395.1"
                     /translation="MPIKKFTLFVVLVLLFAEINFSNAQETIDNKKLSLKKLSEKISE
                     ADDADKIFLKLNSGKSKEFSSSSFSKITRSNGFSLSKEFDNTTPGKNDFKSLSDVSTG
                     LSKGKVDISKYFPPDYKPWESEKHFWWGAGEGVLLNIFIPWAMARYGRTWDSTSEERW
                     PFIGFQSIWNNLNNGWNYDGDNFLTNMFSHPFGGNLFYNSGRTNGMNFWESSAFALGG
                     SFIWETFMETNQPAINDWVITGLGGTAFGEVLYRLSTLATDNTARGMKRVWTEFAGGI
                     FNPARFLTRLVNGEISRVFPNPTWRTPKDLNLNLDAGTRVLINDSTDIKELEGIFELN
                     VEYGDPYRTQHSTPFSHFKYNVQIASSSPNLTGMTVMGSLFAIELDENENTNHTMETS
                     LNYNYLNNPGFLYGNVAITEQLNSQFKLNDHGWHVRSKLGVRLIPMGGTPDDYFLDST
                     DGRNYDMGQGLGAVGKVSLFSEGWEVFTAQYTMDYLWTQSEPSFSKHFLQGGEFSFQL
                     PVKEYFVIGFAGGYYKRKSYYHYPSGFFSYPTPDVSFQTPMIRVFFKTRLI"
     gene            1829..2476
                     /locus_tag="PLX80_06065"
     CDS             1829..2476
                     /locus_tag="PLX80_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20396.1"
                     /translation="MKTILLFLILSISGLTYSQQSNTPKKLSDISKLNTDKKGGYTQL
                     NDQVFVSYSYIRNLGKFGDIYESSNGITLNYGKYFPNNWLVVLRTGYISEKLREGIDS
                     GGYSNFNVYPLHIGGRYYVYKNTIMPYFSFMNGLNFISTSNYLGDPEIEDQFLLRYAF
                     QVGFGIDVKFTKNMGMNLNINFNNSFYKDEDIFDQTNSQMMTGFEYNFGFMYDFK"
     gene            2695..3720
                     /locus_tag="PLX80_06070"
     CDS             2695..3720
                     /locus_tag="PLX80_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20397.1"
                     /translation="MKKILYICGSRNQTTMLHQISKNLEGYDHHFSAFYSDGKEKFFA
                     KFGVLDFSVLGGRFRRQTEQYLQENDLQMDYEGKKYDYDLVVICSDLIVAKNILNKKV
                     IMIQEGMTDPENIIYHMTKKLGSVRYFGGTAVTGLSDVYKYFCVASEGYKDHFIKKGI
                     KPEKILVTGIPNFDNCAASLNNDFPHKKYLLACSSDMRETHKYENRKKFIQKAVMLAG
                     GRQIIFKLHPNEYVPRASREINKWAPGSLIFSTGNTNEMIANCDILLTSYSTVVYVGL
                     ALGKEVHSDFDLEELKRLLPIQNGNTSARKIANICQSVIKEIPLPEIENSIYKEFNIP
                     KIAFDKL"
     gene            3720..4532
                     /locus_tag="PLX80_06075"
     CDS             3720..4532
                     /locus_tag="PLX80_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQY20398.1"
                     /translation="MTNSPRIAIVIQARTGSSRLPEKIMLPLAGKPMLVRMYERVAAS
                     KTPKEIIVATTTDVEDDVVVELCETNGIKYFRGHPTDLLDRHYKTGIEFGAEVVVKIP
                     SDCPLICPDVIDNVLNLYLEDPDRFDFVSNLHPATHPDGNDVEVIPMKILEQVLNEAD
                     RDFEREHTTPYIWERPERFRIGNLEWDTGLDFSMIHRFVLDYEEDYEFIKKIYDELYE
                     NKNDFKLCDIMMLLDRKPELKEINSIRAGINWYRNYFDELKTISKDQFKKSN"
     gene            4720..>5565
                     /locus_tag="PLX80_06080"
     CDS             4720..>5565
                     /locus_tag="PLX80_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQY20399.1"
                     /translation="MEKETKNQLENTAYKVREHIVRMSGDGGCFIGASLSCADLIVYL
                     YKEFLNINKDNLNDPERDYLFLSKGHDVPALYGTFAEIGFIEKDRLKNHLKTNDFIYW
                     HPNTNIPGIEFHSGSLGHLLSVAIGVAIDCKIRGQKNRIVVILGDGELDEGSIWEAAL
                     VAGAKKLDNLIAVVDRNKFQANIETEKLIPLHPIADKFDAFGWKTRSCDGHDFADMEK
                     AFSEFPIENGSPSLVIAETVRGKGLPSIEARADRWFVNFKSEEVEMLLKELHGEGKAK
                     LTSDTI"
     assembly_gap    5566..5665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5771..6673
                     /locus_tag="PLX80_06085"
     CDS             5771..6673
                     /locus_tag="PLX80_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQY20400.1"
                     /translation="MTYEDILLDLINEDDRFMVLTAENRAAIRNLTDKIGDKFLDTGI
                     TEQTLVGAACGLALRGRIPVAHALATFLTMRAFEFIRSDVGISGLPVKLVGGFSGFLS
                     EANGPTHQAIEDVSIMRGIPNVNVFCPADEEDMLIGLTEVLKSNSPFYIRYNNLKPAV
                     RHSKDFEIGKAEIISEGKDVTILVYGILLKQALEAKKILGAKGISTGIINMRCLKPID
                     EDAILNAAKNSDILVTLEDHFLTGGLYSILAEIFLKNEITKKVIPFALDNKWFKPALL
                     NDVLEYEGFTGEKIADRILKKISV"
     gene            6817..8148
                     /locus_tag="PLX80_06090"
     CDS             6817..8148
                     /locus_tag="PLX80_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY20401.1"
                     /translation="MPNTIKFNSEFPDISESDKLFKRSEGLIPAYSQTLAKGPTQWIK
                     GIAPKFLKKGKGSHVWDADGNEFIDFNMGIGPIVLGYCYDKVDDAIRRQLEDGITFSM
                     THPLEVKLSEKIRDVIPNAESVRFSKTGCDVTTAAVRVARAFTKREKVLCCGYHGWHD
                     WYIGVTDRDAGIPAASKDLTFTFNYNDIESVIKSIDNETACVILEPFVFEEEKNGFLK
                     QLKEVCQKNGTLLIFDEMWTGFRIATGGAQEYFGVTPDLACYSKAIANGMPISALTGR
                     ADVMRLFDKDVFFFTTFGGEALSLAAAMATIDEIKEKNVTAVLSDQGKKLKDGYNKIV
                     SDLGMDFTKCIGYNCRTIMTFDAKAGNPLEMKSLVQQEMIKRGVLWCGFHNMCYSHSD
                     EDVSYTLKAYEDVLPILKKAVGENKVKDFLKGEPVEPVFRKTSNFNMKPLK"
     gene            8257..9132
                     /locus_tag="PLX80_06095"
     CDS             8257..9132
                     /locus_tag="PLX80_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQY20402.1"
                     /translation="MSKIKIGNRYIGDNEKVFIIAEIGINHNGSLRLAKRLIKEAFQA
                     GAEAVKFQKRTPELCVPVDQWYIERDTPWGRMTYIDYRFKVELSLQDYSEIDRYCKKL
                     GIIWSASCWDEDSVDFIEQFDVPFYKAASASLTDQSLLNKKRSTGKPLMISTGMSTLK
                     EIKDAVKFLGKENLMIAHSTSSYPCKLEELNLKMITTLQKMYIDIPIGYSGHETGLSP
                     TWAAVALGANFVERHITLDRAMWGSDQAASVEPGGFKRLVENIRDIEKALGDGIKKIY
                     DSEVGQRKKLRRAIS"
     gene            9378..10214
                     /locus_tag="PLX80_06100"
     CDS             9378..10214
                     /locus_tag="PLX80_06100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY20403.1"
                     /translation="MSLQIWSLIIISTAAVMMVVFEKLYPYTKGQKIFREGFFNDIIL
                     YSIVQSYLLGLIIFGFLNWIKDNTDLYKYSLIGDWPVWLQVMFFVITHDFYIYWFHRW
                     QHNNKFLWRTHEAHHSPKTVDWLSGARSHSLEILINQSVEFAPIILLGAAPEVAIYKG
                     MISAIWGMFIHSNIDIRLGKLQRFINGPEMHRWHHSDEVGREFQNNYGTKLAVWDWIF
                     GTAFLPDPKVRKPKQFGLSDTPDYPLSIDENKISNIHKVIWADIVSYIKQHSYAFRKF
                     KK"
     gene            10508..11482
                     /gene="moaA"
                     /locus_tag="PLX80_06105"
     CDS             10508..11482
                     /gene="moaA"
                     /locus_tag="PLX80_06105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQY20404.1"
                     /translation="MLTDSFGRTHNYLRISVTDRCNLKCVYCMPELGQLHDMKNKVMS
                     ADEIFEIASVFSELGINKIRITGGEPFVRKDIDDIFCKLSKLPVELAVSTNAVLIDRH
                     IDVLKSCNVNNINISLDTLDPDEFLAITKSNDLEKILININLLLENDFNVKINFVVMK
                     GVNEDAVNEIAGLTKDYKAEVRFIEFMPFSGNNWTREKVFSHDEILNKIINEYEIFKL
                     PDEIHDTSKKYGITGHAGVIGIISTITKPFCDGCNRLRLTADGKIKNCLFSKGETDIL
                     STFRKGDDIIPMIISDLKLKFAERGGQFDGEEFDAKDFENRSMISIGG"
     gene            11482..12219
                     /locus_tag="PLX80_06110"
     CDS             11482..12219
                     /locus_tag="PLX80_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY20405.1"
                     /translation="MEPSYFLLIPLLFIVAVMYSSVGHGGASGYLAVMSILSIAPETM
                     RTSALILNLFVSGIAFYGFRRSGHFRKELFIPFAVTSVPFAFIGAGFPADTDLYKIIL
                     GIILLFAALKISGMVDKFKPDIKINYSLSLALMIGAILGFVSGMIGIGGGIILSPLIL
                     LFRWADVKETAAISALFIFLNSGAGLFRISLSGLNVGSELIYLIIAVIAGGFIGSYWG
                     SFVAENKTLRYVLSVVIFAASVKLIFI"
     gene            12228..12461
                     /locus_tag="PLX80_06115"
     CDS             12228..12461
                     /locus_tag="PLX80_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQY20406.1"
                     /translation="MKLKILYFGVTADITCKDSESVSDIPDTNSLNDMLIGLYPELKN
                     ANYRIAVNLEMIKENTVMKDGDEVALLPPFAGG"
     gene            complement(12751..14229)
                     /locus_tag="PLX80_06120"
     CDS             complement(12751..14229)
                     /locus_tag="PLX80_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY20407.1"
                     /translation="MENNSHIKKLFSHTVIYGLGIVLNKSVNFILLPVYTRYYTPQEI
                     GMLTLLQSISFFLMVIYALGMETSFMKFFIEEKDVKKRSEIYSATLIFLLIFTAIISS
                     VIFFFSESIVTVFDFTDIDTSILLLKILCVLMVADTIYRFPLLLFRAELKAKTYAYIN
                     LLTFVVNIIANIVLIVFLKIGIEAILYSYLLSVIVTLVVSIIVTGKYLTMSISLKRIK
                     EMLKFGNKFIYIGLCLILIDMSDRFFLKYFFNEKVVGIYWANYRLATVMSLVIAAFKF
                     SWTPYFLNLSDHPENKKIISNIFTYFCFAGLSMFLIFSLLIDQFVQINILGFQFLDTN
                     YMYGLTIVPLVLLAYFFSGAYSTMNAAPFFTDKTFLILFISITGLLINTALNFLLIPI
                     LEMNGAALSTLITYLAMFLIMYYYSQKIYKIDYEWKKIFRIGLLTAVFFLISYFAVNN
                     ITDSNSIKIILNFLLISIFFFVINSTGIIKLRSVMNVIFKKK"
     gene            complement(14219..15295)
                     /locus_tag="PLX80_06125"
     CDS             complement(14219..15295)
                     /locus_tag="PLX80_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20408.1"
                     /translation="MSLKILHIAPQNFAGMPFEFVKMHRKYGHESSLVTLYKNTLNFE
                     EDICLNLPLPSGKGAKAWRDSKAEEAVENKLKYAGPKNLAEKIYFNLRDQKNKNVINE
                     TIEKHSLYDFDVYHFDGGMDLFRDIRFAKELEKRNKKIVCCYFGSDLRSRGVFREMDE
                     MSDLNLTVEFDHLALHPNINFTFFPFDTDAFVIKEKSNKKIKIIHSPTNRKFKGTDKI
                     LEIINEVEKEREIEFILLENTDREKVLEIKKECDLAVDQVGGELGGSGYGKNSIENLS
                     MGIPTFTEFTNDYLLFLERNPFIHSTVDTLKDNILKLIDNKDLRKDVAVKGREWVEDF
                     HSFNAVNKKLNEYYSKFNITGGGK"
     gene            complement(15467..15991)
                     /locus_tag="PLX80_06130"
     CDS             complement(15467..15991)
                     /locus_tag="PLX80_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQY20409.1"
                     /translation="MKKIKSEKKLKRKKPDLSKIKFILMDLDGCLTTGHILYTSSGDS
                     MKMFHTHDGFGIVRARELGLKFAVISGMSAKENKMRVDKLKIHHLYENIDDKKIPFEE
                     LKKLYDMKNEEFAYIGDDEFDLPLLMEVGFSCCPNSAVDEVKKKVDYICKKNGGEGAV
                     REMVDMILKKKKLL"
     gene            complement(16016..16663)
                     /gene="nth"
                     /locus_tag="PLX80_06135"
     CDS             complement(16016..16663)
                     /gene="nth"
                     /locus_tag="PLX80_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQY20410.1"
                     /translation="MKKPALKKLSSEIVSRLAKEYPDVKCHLDFNSPFELLVSTVLAA
                     QCTDIRVNMVMVPLYKSKYNKPADIIKDGIDNFRENIKSINFFNNKAKAVLSICNSLV
                     NDFGGKVPDNMEDLTSLAGVGRKSASVILGNCFGKNDVIIVDTHLKRVAARLGLIEND
                     NPEKIEAELKEIIPNDEQFYFSMRIGEHGRQVCKAKKPDCENCVMNDICVSAFKV"
BASE COUNT         5525 a         2712 c         3164 g         5217 t
ORIGIN      
        1 aaattttact gataaacgtt atggacggct gtgggtaaga taacagaagg aaatgcttta
       61 taagttttgc attaatattg attttattat atttgctcaa tattttataa ccccctaatc
      121 tctaaaatca gatatgccta ttaaaaaatt tacacttttt gttgtgctag ttttgttatt
      181 tgcggagata aatttttcaa atgctcagga gacaattgac aataaaaaat tgtctttgaa
      241 aaagctttcc gaaaagatct ctgaggcaga tgacgcagat aaaatttttt taaaactgaa
      301 ttcgggaaag tcaaaagaat ttagttcttc ttcattttca aagatcaccc gttcaaacgg
      361 attttcactt tccaaagaat ttgacaatac cacgccgggg aaaaatgatt tcaaatcact
      421 ttctgatgta tcgacaggtt tgtcaaaagg taaagttgat atttcaaaat attttccgcc
      481 tgattacaaa ccatgggaat ctgaaaagca tttctggtgg ggagccggcg agggtgtatt
      541 gttaaatatc ttcattccgt gggctatggc aagatacgga aggacatggg attcgacatc
      601 agaggaaaga tggcctttca taggatttca gtctatttgg aataatttaa ataacggatg
      661 gaattatgac ggagacaatt ttctcacaaa tatgttctcc catccattcg gtggtaattt
      721 attttataac agcggcagga cgaacggaat gaatttctgg gaatcatctg ctttcgctct
      781 cggcggaagt tttatctggg agacatttat ggaaacaaac cagcctgcta tcaatgactg
      841 ggtaattaca ggattgggag gtacggcatt tggtgaagta ctttacagac tttcgacttt
      901 agctactgat aatactgcga gaggaatgaa gagggtatgg acggaatttg caggcggtat
      961 ttttaatccg gcaagatttc tgacaagact agtgaacggg gaaatttcaa gagtctttcc
     1021 gaatcctaca tggagaactc cgaaagatct taatctgaat ctcgatgcag gtacaagggt
     1081 gcttatcaat gattcaactg acattaaaga acttgaaggt atatttgaat taaatgtcga
     1141 atatggtgat ccttacagaa ctcaacattc tacgccattt tcacatttca aatataatgt
     1201 tcagatcgca tcttcttctc cgaatcttac agggatgaca gtaatgggta gtttgtttgc
     1261 aatcgaatta gacgagaatg aaaacactaa tcatacgatg gaaacttctt tgaattacaa
     1321 ttatttaaat aatcccggat ttttatatgg taatgttgca atcacggaac agttaaattc
     1381 tcaatttaag ctgaatgatc atggctggca tgtaagatct aaacttggag taagattaat
     1441 tccaatgggc ggaacgccgg atgattattt ccttgattct acagacggca ggaattatga
     1501 catgggtcag ggacttggag ctgttggtaa agtatctctg ttctcagaag gctgggaagt
     1561 atttactgct cagtatacta tggactactt atggacacag agtgaacctt ctttttcaaa
     1621 acatttttta cagggcggag agttttcatt ccagctgcct gtaaaagaat attttgttat
     1681 aggatttgcg ggcggttatt acaagagaaa aagttactac cattatccat caggattttt
     1741 ctcttatcct acacctgacg taagtttcca aacaccaatg ataagagttt tctttaaaac
     1801 aagattaata taaactgcaa ataattatat gaaaacaatt ttattatttt taattttatc
     1861 aatttccgga ctgacatatt cacagcagtc caatacacct aaaaaattat ctgacatcag
     1921 taaattaaat acagataaaa aaggtggata cactcaactt aatgatcagg tatttgtcag
     1981 ttattcctat ataagaaatc tcggaaaatt tggggatatt tatgaatcca gcaacggtat
     2041 cactttaaac tacggaaagt attttcccaa taactggctg gttgtacttc gtacaggtta
     2101 tatcagcgaa aagctgagag aaggtataga ctcaggagga tacagtaatt ttaatgttta
     2161 tcctttgcat atcggaggca gatattatgt ttacaagaat actattatgc catatttttc
     2221 ttttatgaat ggtctgaatt ttatttcgac aagtaattat ctcggcgatc cagaaatcga
     2281 agaccagttt ctcttaaggt atgcatttca ggtcggattt ggtattgacg tgaaatttac
     2341 aaagaatatg ggaatgaatc ttaatataaa ctttaataac agtttctata aggatgagga
     2401 tatttttgat cagactaatt cccagatgat gaccggattt gaatataact tcggctttat
     2461 gtatgatttt aaataagatc atactctaac ttatttaagt aatttttaaa agatttttgc
     2521 tttctgtttt tcagcgtgtt tattaattta aactctgtag gcagaaagca tttttattct
     2581 ggacataaag tttatctcag atgaagaaaa tcaaatctgt ataaaagagg gtaagaagtt
     2641 tcaaaaattg aatgtaataa aacgtatgat ttttgtagtt tcaccgaact gaatatgaaa
     2701 aaaattctat acatctgcgg ttcaaggaat caaactacta tgctccacca gatctctaaa
     2761 aatctggaag gatacgatca tcatttttct gcattttatt ctgacggtaa ggaaaaattt
     2821 tttgcaaaat tcggagtact tgatttttct gtactcggcg gcagattcag acggcagact
     2881 gagcagtatc ttcaggagaa tgatctgcag atggattatg aaggaaaaaa atatgactat
     2941 gaccttgtag tgatatgcag tgatctgatt gttgccaaaa atattctgaa taaaaaagtt
     3001 ataatgatac aggagggaat gaccgatccg gaaaatatca tttatcatat gactaaaaag
     3061 ttaggttctg taagatattt cggaggaaca gcagttacgg gactttcaga tgtttataaa
     3121 tatttttgtg tagcttcaga aggatacaaa gatcatttca taaaaaaagg aataaaacct
     3181 gaaaaaatat tagtaaccgg aattccaaat tttgataact gtgcagcttc tctcaataat
     3241 gactttcctc ataaaaaata tcttctggct tgctcctcag atatgagaga gacacataaa
     3301 tatgagaaca ggaaaaaatt tatacaaaaa gctgtaatgc ttgccggcgg cagacagatc
     3361 attttcaaac tgcacccaaa tgaatatgta ccaagagcaa gcagagaaat aaacaaatgg
     3421 gctccgggtt cgttgatttt ttcaacagga aatacaaatg aaatgattgc taactgtgat
     3481 atactgctga ctagttattc tacagttgtt tatgtcggac ttgctttggg aaaagaagtt
     3541 cactcggatt ttgatctcga agagcttaaa agacttctcc cgatccaaaa cggaaataca
     3601 tctgcccgta agatcgcaaa tatttgtcaa tctgtaatta aagaaattcc tctgcctgaa
     3661 atagagaatt ctatttataa agaattcaac attccaaaaa tagcatttga taaattataa
     3721 tgacaaattc tccgaggata gcaatagtaa tacaggcgag aacaggttcg agcaggcttc
     3781 cggaaaagat aatgcttcca ctcgcaggca aaccaatgct tgtccggatg tatgaaagag
     3841 tagcggcttc aaaaactcca aaggaaatta tagtcgcaac tacaaccgat gtggaagatg
     3901 atgttgtagt agaactttgt gaaacaaatg gtataaaata tttccgtggt catccgactg
     3961 accttcttga cagacattat aaaacaggaa tagaatttgg agctgaagtt gttgttaaaa
     4021 ttccttcaga ctgtccgctg atttgtccgg atgttataga taacgttctg aatctgtatc
     4081 ttgaagatcc ggatcgattt gattttgtaa gcaacctgca tccggctact caccctgatg
     4141 gtaatgatgt ggaagtcatt cctatgaaaa tacttgaaca ggtattgaat gaggcagaca
     4201 gggatttcga aagagagcat accaccccat atatctggga aagacctgaa agattcagaa
     4261 ttggaaatct tgaatgggat accggtttag atttttcaat gattcataga tttgttctgg
     4321 attatgaaga agattatgaa ttcataaaaa agatctatga cgagctttat gaaaataaaa
     4381 atgattttaa actttgtgac attatgatgt tgcttgacag aaagcctgaa cttaaagaga
     4441 tcaattcaat acgtgcagga ataaactggt acaggaatta ttttgatgaa ttgaaaacga
     4501 ttagtaaaga tcaatttaaa aagagcaatt aaaaattaag agttacgatt taaaaattac
     4561 gagttgcgag ttaagagtta cgaaattaat ttttaaaaac ctcgatggtt gaaagtatta
     4621 tagattataa aattaatttt taaaaacccc cgaaggggtg acactattat agattaaaaa
     4681 aagtattttt aaaaatccca aaggggtgac attattatta tggaaaaaga aactaaaaat
     4741 caattagaga ataccgcata taaagtaaga gagcatattg tcagaatgtc gggagacggc
     4801 gggtgtttta tcggagcatc actttcatgt gccgatctga tagtttattt atataaagaa
     4861 tttttgaata taaataaaga taatctgaat gatcctgaaa gggattattt atttctttca
     4921 aaaggccatg acgtgcctgc tctatacggg acattcgccg aaattggatt tatagaaaaa
     4981 gacagattaa aaaatcatct aaagacgaat gattttattt actggcatcc gaacacaaac
     5041 attcccggaa ttgaatttca ctcaggatcg ctcggtcatc tgctgagcgt agccattgga
     5101 gttgcgatag attgtaaaat acgcggacag aaaaaccgta ttgttgtaat tctcggagac
     5161 ggtgaattgg atgaaggttc tatatgggaa gctgcactgg tagcaggtgc aaagaaactt
     5221 gataatttaa ttgcagtagt ggacaggaat aaatttcagg caaatatcga aacggaaaag
     5281 ctgataccgc tgcatccaat tgcggataag ttcgatgctt tcggctggaa aacccgctca
     5341 tgtgatggtc atgattttgc agatatggaa aaagcttttt cggaatttcc gatagagaac
     5401 ggcagcccgt cacttgtaat agctgaaaca gtcagaggca aaggattgcc gagtatagaa
     5461 gcgcgtgctg acagatggtt tgtgaatttc aaatctgaag aggtcgaaat gcttttaaaa
     5521 gaactgcatg gtgaggggaa agcaaaactt acttccgata ctattnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnntttca aatattttat tgtaaaaagt aatatttcaa
     5701 catcagaatt ttgaaattgt atagttaaga cattattaat agtttagtaa aattaaaata
     5761 cacatcatta atgacatacg aagatattct tctagatctg ataaatgaag acgacaggtt
     5821 tatggtgctg accgctgaga acagggcagc gataagaaat ctcacggata aaatcggaga
     5881 taaatttctc gatacaggaa ttacagagca gacacttgtc ggtgctgcat gcggactagc
     5941 gcttagagga agaattccgg ttgcacatgc acttgcgaca tttcttacga tgagagcttt
     6001 tgaatttatc cgttcggatg tcgggatcag cggactacct gtaaaacttg taggcggatt
     6061 ttcgggattc ttatctgaag caaacggacc gactcatcag gcaatcgaag atgtttctat
     6121 catgagaggc attccgaatg tgaatgtgtt ttgtccggca gatgaagagg acatgctgat
     6181 tggacttacc gaagtattaa aaagtaattc acctttttat ataagatata ataatctgaa
     6241 acctgctgtc aggcattcaa aagattttga gattggaaaa gctgaaatta tttcagaagg
     6301 aaaagatgtg acaatacttg tatatggaat ccttttgaag caggcacttg aggcaaaaaa
     6361 aattctcggg gcaaaaggaa tttccacagg cataataaac atgcgctgcc ttaagccaat
     6421 tgatgaagat gcaatattaa atgctgcaaa aaattcggat atacttgtaa ctcttgaaga
     6481 tcattttctg acaggcggac tttattctat actggctgag atttttttaa aaaatgagat
     6541 taccaaaaaa gtaattccgt ttgcgcttga taataaatgg ttcaagcctg cattgctgaa
     6601 tgacgtgctg gagtatgaag gatttaccgg agaaaagatc gctgacagaa tcctgaaaaa
     6661 aatatcggtt taaaaattta atttgaattc agtgtccttt tttccgcaaa gtccgaagag
     6721 tacgtgactc tgcgggaaca aatttataat actaaataac attaactgat gaaaaattta
     6781 ttttttatct aaacaatttt aactgtaaat atttatatgc caaatactat aaagtttaat
     6841 tctgaatttc ccgacatttc cgaatcagat aaattattca aacgctcaga aggtctaata
     6901 cctgcttatt cacagactct tgcaaaaggt ccgacacaat ggataaaagg tatcgcaccg
     6961 aaatttctca aaaaaggaaa aggctcgcat gtctgggatg ctgacggaaa tgaattcata
     7021 gattttaata tgggcatagg tccgatagta cttggatact gctatgataa agtagatgat
     7081 gcaatacgca gacagctcga agacggaatt actttttcca tgacacatcc gctcgaagtg
     7141 aaactgtcag aaaagataag agacgttatt ccaaatgcag agtcagtcag attttccaag
     7201 accggctgcg atgtgactac tgctgcagtt cgtgttgcaa gagcatttac caaaagagaa
     7261 aaagtcctgt gctgcggata ccacggctgg catgactggt atattggtgt gacagacaga
     7321 gatgcgggaa ttcctgctgc ttcaaaggat cttacattca cttttaatta caatgacata
     7381 gagtctgtga taaagtctat cgacaatgaa actgcttgtg taattcttga gccgtttgtt
     7441 tttgaagaag aaaaaaatgg ttttctgaaa cagctaaaag aagtttgtca aaaaaatggt
     7501 acacttctga tctttgatga aatgtggaca ggatttagaa tagcaacagg aggagcacag
     7561 gaatatttcg gggtcacgcc tgatcttgca tgttactcaa aagccattgc caatggaatg
     7621 ccaatctctg cattaacggg aagagccgat gtgatgagat tgtttgacaa ggatgttttc
     7681 ttttttacaa cttttggtgg agaagctttg tcactggctg cagctatggc aactattgat
     7741 gagatcaaag aaaaaaatgt aacagctgtt ttatcagatc agggaaaaaa acttaaagac
     7801 ggttataata aaattgtgtc tgatctggga atggatttta caaaatgtat tggatataac
     7861 tgcaggacga tcatgacatt tgatgctaag gcaggaaatc cgctggagat gaagtcactc
     7921 gttcagcagg aaatgataaa gcgcggagta ttatggtgcg gatttcataa tatgtgttac
     7981 agtcattctg atgaagatgt gagttatact ctgaaagcat atgaagatgt gttgccgatt
     8041 ttaaaaaaag ctgttggaga aaataaagta aaagattttt taaaaggtga accagttgag
     8101 ccggtgttta gaaagacaag taattttaat atgaaaccgt tgaaataatt aggaattaaa
     8161 aattaagaat taaaaatttt taattagtta ataaattcat gatgtttctt tattaaattt
     8221 ttagtaagtt tataaaaata attaaacctt aaatttatgt caaaaataaa aatcggaaat
     8281 agatatattg gagataatga aaaggttttt ataattgccg agatcggaat aaaccataac
     8341 ggttcgctca gacttgctaa aagacttatc aaagaagcat ttcaggcagg tgcagaggcg
     8401 gtaaaatttc agaagagaac acctgaactt tgcgttccag tagatcaatg gtatattgaa
     8461 cgcgacacac catggggaag aatgacttat attgattaca gattcaaagt agaattgagt
     8521 cttcaggatt attctgagat tgacagatat tgtaaaaagc tcggaatcat ctggtcagct
     8581 tcgtgctggg atgaagattc agttgatttc attgaacagt ttgatgtgcc tttctataaa
     8641 gctgcttcag cttcattaac tgatcagagt cttttaaata aaaaaagatc aacaggtaaa
     8701 cctctgatga tctcaaccgg tatgtcaact ttaaaggaga ttaaagatgc agtaaaattt
     8761 ttagggaaag agaatttaat gatcgcacat tccacttctt cctatccgtg taaattagaa
     8821 gagcttaatt tgaaaatgat tacaactctt caaaaaatgt acatcgatat tccgattgga
     8881 tattccggac atgagacagg attgtcaccg acctgggcag cagttgcact cggagcaaat
     8941 tttgtcgaaa ggcatattac tcttgacagg gcaatgtggg gcagtgacca ggctgcatcc
     9001 gtcgagccgg gcggattcaa aagacttgta gaaaatatcc gtgacattga aaaagctctg
     9061 ggtgatggaa taaagaaaat atatgacagt gaagttggac agaggaaaaa actgcgaaga
     9121 gcaatatctt aatttcaaat tgcaaatatc aaattacaaa taaaaagcgg ctaatttttt
     9181 aaattgtttt aactatagat cttaacagac gcagctataa tagctgcgtt tttattttaa
     9241 ggttaatcaa attttatttt aaaaagaatt tattaaaaac tatttgtaat ttgacatttg
     9301 caatttgtaa tttgaatttt tttaaagtct tgaattaatc agtttaatta aaaaatctgc
     9361 ttcgttattt ttgcactatg tcacttcaga tctggtcatt aataataatt tcaactgctg
     9421 cagttatgat ggtagtcttt gaaaaacttt acccttatac aaaaggtcaa aagatattcc
     9481 gagaaggttt ctttaatgat attatcctgt actcaatcgt gcagagctat cttctcggac
     9541 ttatcatatt cggatttctg aactggataa aagataacac agatctatac aaatacagtc
     9601 tgataggcga ctggcccgtg tggctgcagg tgatgttctt tgtgattaca catgattttt
     9661 atatttactg gtttcaccgg tggcagcata ataataaatt cctatggcgt acacatgagg
     9721 cacaccactc tcctaagaca gttgactggc tttcgggagc gagatcacat tctcttgaaa
     9781 ttcttataaa tcagtcggta gaattcgcgc ctatcattct tctcggcgca gcacctgagg
     9841 tcgcaattta taaaggaatg atcagcgcaa tttggggaat gttcatacat tcgaatattg
     9901 atataagact cggaaaactt cagcgtttta taaacggtcc tgaaatgcac cgctggcacc
     9961 actctgatga agtcggcagg gagtttcaaa ataattacgg aactaagctt gcagtctggg
    10021 actggatatt cggtactgca tttcttcctg atccgaaagt cagaaagcca aaacagtttg
    10081 gattaagcga cacaccggat tatcctctgt caattgatga aaataaaatt tcaaatatcc
    10141 acaaagtaat ctgggctgac atagtaagct atattaaaca gcattcatac gctttccgta
    10201 aattcaaaaa ataaattttt tacttctaag tctcaaaggc tctaagtttt ttaatttaac
    10261 tcttaagatt ttttagaata aaaaataatg attttaaaaa aacggttttt ttggagccgc
    10321 agagtcactt gctcttcgga ctttatggat attgaagata gagttttcaa gttttaaatc
    10381 gtttaagttt taaagcctta gagccttaga gtcttagaga ctttgaggca aatcaatata
    10441 tttccaaaat atctgaaact gattttcatt ttcaaaagag ttatcttaat atctaaaaaa
    10501 atcatatatg ctgaccgatt ctttcgggcg aacacataat tatctgagaa tttccgttac
    10561 tgaccgctgc aatctgaagt gtgtctattg catgcctgag ctaggtcaat tgcatgatat
    10621 gaaaaataaa gtaatgtccg ctgatgagat atttgaaatt gcatctgtgt tcagtgagct
    10681 tggcataaat aagatacgca ttaccggcgg tgaacctttt gtaagaaaag acatagatga
    10741 tattttttgt aaactttcta aactgcctgt tgagttagct gtttcgacca atgcagtttt
    10801 gatcgacaga cacattgatg ttttaaaaag ctgtaatgta aataatatta acatcagcct
    10861 cgatactctt gaccctgacg aatttttagc aattacaaaa agtaacgacc ttgagaaaat
    10921 tcttatcaat ataaatcttt tgcttgaaaa tgattttaat gttaagatta atttcgtcgt
    10981 gatgaaaggt gtaaatgaag atgccgtgaa tgaaattgcc ggattgacaa aagactataa
    11041 agcggaagta agattcattg agttcatgcc attcagcgga aataactgga cacgtgaaaa
    11101 agtattttca catgatgaga tattaaacaa gatcatcaat gaatatgaaa tttttaaact
    11161 acccgatgaa attcacgata catcaaaaaa gtacggtatt acgggacatg caggagttat
    11221 aggaattatc agcacaatta ctaaaccatt ctgcgatgga tgcaacaggc tgcggctaac
    11281 tgctgacgga aagatcaaga actgtctttt ttcaaaaggt gagacagata ttctttcgac
    11341 tttcagaaaa ggagatgata taattccaat gataatttct gatctgaaat taaaatttgc
    11401 cgaacgggga ggacagttcg atggtgaaga atttgatgca aaagattttg aaaacagatc
    11461 aatgataagc atcggtggct gatggaacct tcttactttt tattaattcc acttcttttt
    11521 atagtagcag taatgtactc ctcagtcggt cacggcggcg cgagcggcta tcttgctgtg
    11581 atgagcattc tcagtattgc tcccgagact atgcgaactt cagcattgat cctgaatcta
    11641 tttgtttcag gaattgcatt ttacggattc agaagatccg gacatttcag gaaagaactt
    11701 ttcattccgt ttgcagtgac atcagttccg tttgcattta tcggggcggg atttcctgct
    11761 gatacagatc tttataaaat tatacttggc ataatattat tatttgccgc attaaaaatt
    11821 tcaggaatgg ttgacaaatt taaacctgac attaaaataa attacagttt atcattggca
    11881 ctaatgatag gtgcaatact cggatttgta tcaggcatga tcgggatcgg aggaggcatt
    11941 atcctgagtc cgttgatact tttattcaga tgggctgatg taaaagagac tgcggcaatt
    12001 tcagcgttgt ttatatttct gaattcaggt gcaggactat ttagaatttc gcttagtggt
    12061 ttgaatgtcg gttcagagtt gatctatctg attatagctg tgatagcagg cggttttatc
    12121 ggttcatact ggggaagttt tgttgcggaa aataaaacac tcagatatgt tttgtcggta
    12181 gttatctttg ctgcttctgt aaaattaatt tttatataaa atttaaaatg aaattaaaaa
    12241 tattatactt cggggtgacc gctgacatta catgtaagga ttcggaatct gtttcggata
    12301 ttcctgacac aaattctctc aacgatatgc ttatcggttt atatcccgaa cttaaaaatg
    12361 caaactaccg cattgcagtc aatctcgaaa tgataaaaga aaatacagtt atgaaagatg
    12421 gagatgaagt tgctctgctg ccgccatttg cgggaggtta attaaatttt taactgaata
    12481 caacctcagt gtaaattata atgcctgaaa ctcctgtact tacttcaaag gatgttgtta
    12541 aaattattga aaaaacggat tcaaattagt ctgaactaaa aggagtcatt atatatttag
    12601 aaacgagcaa tcgaaaaaga tgactgttgt tccatttcat taaaagaatt taccaaaagg
    12661 aactcttctc gagattttaa agcaagcagg aattgataaa agtgaaataa taaaatagta
    12721 tttcaactta gatatattat cagatattaa tcattttttt ttaaagatta cgttcataac
    12781 tgacctgagc tttatgattc ccgtagaatt tatcacaaaa aagaagatcg atattaaaag
    12841 aaaattgagt attattttaa tgctattact atcagtaata ttatttacag caaaataact
    12901 tatcagaaaa aatacagcag tcagtaatcc tatcctgaat atttttttcc attcataatc
    12961 aattttatag atcttctgag aataataata cattatcagg aacattgcaa gatatgtgat
    13021 cagagttgac aacgcagctc cgttcatttc taagatcggt ataagcaaaa agttcagcgc
    13081 tgtattgatc aagagtccag tgatcgagat aaataaaatg agaaatgtct tgtcggtaaa
    13141 gaacggcgca gcattcatag tagaataagc accgctgaaa aaatatgcaa gcagtaccag
    13201 cggaacgata gtaagtccat acatataatt cgtatcgaga aactgaaacc ccagaatatt
    13261 gatctggaca aactgatcta tcagcaatga aaaaatcaga aacatcgaaa gtcccgcaaa
    13321 acaaaagtat gtaaatatat ttgagatgat ctttttgttt tccggatgat cactgagatt
    13381 tagaaaataa ggcgtccatg aaaatttaaa tgcagcgata acaagactca tgacagtagc
    13441 caatctgtaa ttcgcccagt aaataccgac aactttttca ttgaaaaaat atttcagaaa
    13501 aaatctgtct gacatatcta tgagtataag acacaatcct atataaataa atttatttcc
    13561 aaacttcagc atctccttta tccttttcag cgagatgctc attgtaagat actttcctgt
    13621 tacaattatg ctgaccacta atgtaacgat cactgataaa aggtaactgt aaagaatcgc
    13681 ttcgattcct atttttaaaa atactatcag aacgatgttt gcaatgatgt tcacgacaaa
    13741 tgtgagaaga ttgatatatg cgtaagtctt tgccttcagt tcggcacgga agagcagcag
    13801 tggaaatctg taaatcgtat cggcaaccat cagcacgcac aatattttca aaagaagtat
    13861 gctcgtgtca atgtcagtaa aatcgaaaac tgtaactatg ctttctgaaa agaaaaatat
    13921 cacagaagaa attattgcag taaagattaa aagaaatatc agagtggccg aataaatttc
    13981 cgatctcttt tttacatcct tctcttcaat gaaaaatttc ataaaggaag tttccatgcc
    14041 aagtgcatag atcaccataa gaaaaaatga aatagactgc agtagcgtca gcattccgat
    14101 ttcctgcggt gtataatatc ttgtataaac aggaagcagg ataaagttca ctgacttgtt
    14161 cagtactatg ccaagtccgt aaatgacggt atgagaaaat aattttttta tatgagaatt
    14221 attttccacc gccggttata ttgaatttac tgtaatactc attaagtttt ttattgactg
    14281 cattaaagga atgaaagtcc tcaacccatt ctctgccttt gacagcgaca tcttttctca
    14341 gatctttatt atcaatcaat ttcaaaatgt tatctttaag tgtatccact gtggaatgta
    14401 taaaaggatt tctttccagg aaaagcagat agtcatttgt aaattctgtg aatgtgggaa
    14461 ttcccattga cagattctca atggaattct ttccgtagcc gctgccgcca agttcaccgc
    14521 ctacctgatc taccgcaagg tcacattctt ttttaatctc aagtactttt tctctgtccg
    14581 tattttcaag aagtatgaat tcaatttctc tttccttttc gacttcattt atgatctcta
    14641 atattttatc tgtgcctttg aattttctgt tggtcggcga atgaatgatc ttaatttttt
    14701 tattgctttt ttctttaatg acgaatgcat ctgtatcaaa aggaaaaaat gtgaagttaa
    14761 tatttggatg taatgcaagg tgatcaaact ctactgttaa attcagatcg ctcatttcat
    14821 ccatttctct gaatacacca cggcttctga gatcacttcc aaaatagcag catacaattt
    14881 tcttattacg cttctctaat tctttggcaa atcttatgtc acggaacaga tccatcccac
    14941 cgtcgaagtg atagacatca aagtcataaa gcgaatgttt ttctatagtt tcatttatca
    15001 cgtttttatt tttctgatct ctgagattga aataaatttt ttcggcaaga tttttcggac
    15061 cggcatattt taatttattt tcgactgcct cttctgcctt actgtcacgc caggctttcg
    15121 cacctttacc ggaaggcagg ggcagattca gacaaatgtc ttcttcaaag ttaagggtgt
    15181 ttttataaag tgtgacgaga gaagattcat gtccatactt cctgtgcatt ttcacaaatt
    15241 cgaaaggcat ccctgcaaaa ttctgcggcg cgatatgtaa gatctttaat gacaataatt
    15301 ctaaattgaa tttcttggtt ttgcctcaaa ggctctaaaa ctcaaagtta acagttagta
    15361 gttaaaagtt agaaaatttt tctgtttcct cagagtcacg ggcttttcgg actctgaggg
    15421 aagttaaatg aaactttgag tcttagagac ttcgaggcga aataatctaa agcaatttct
    15481 ttttcttaag tatcatatcc accatctctc tcactgcgcc ttcaccaccg ttttttttgc
    15541 aaatgtaatc aacttttttt ttcacctcat ccaccgcact atttggacag caggaaaacc
    15601 caacttccat caacagcggc agatcaaatt catcatcacc gatatatgca aattcttcat
    15661 tcttcatgtc atataatttt ttcaattctt cgaaaggaat tttcttatcg tcaatattct
    15721 cataaaggtg atgtatcttc agtttgtcca ctctcatctt attttctttt gcgctcatgc
    15781 cgctgatgac tgcgaactta agcccgagtt ctctcgctct cacaattcca aagccgtcat
    15841 gagtgtgaaa cattttcatg ctgtcacctg agctggtgta aagaatatga ccagtagtca
    15901 gacatccgtc gagatccatc aaaatgaatt tgatctttga cagatcaggt ttctttctct
    15961 ttaatttttt ttcagatttt atttttttca aacttttttt attattgaaa ttaatttaaa
    16021 ctttaaaagc gctgacacag atatcattca taacacagtt ttcacaatcc ggctttttgg
    16081 ctttgcagac ctgtcttccg tgttcaccga tcctcattga aaaataaaac tgctcatcat
    16141 tcggaattat ctctttcagc tcagcttcaa tcttttcggg attatcattt tcgataagtc
    16201 cgagacgcgc agcaactctc ttaagatgag tatctacaat gatcacgtca tttttcccga
    16261 agcagtttcc tagtatcaca cttgctgact ttctgccgac acctgcgagt gaagtgagat
    16321 cttccatatt gtccggcact ttccctccga agtcatttac aagactgttg cagatcgaaa
    16381 gtacagcttt tgctttgttg ttaaaaaaat ttatcgattt tatattttct ctgaaattat
    16441 caataccatc cttaataatg tcagcgggtt tattgtattt agatttgtat aacggaacca
    16501 ttaccatgtt gacacggata tcagtacact gggcagcgag gacggtggat actaaaagtt
    16561 cgaacggtga attaaaatca agatgacatt ttacatctgg atattctttg gcaagacggc
    16621 tgacaatttc tgatgaaagc ttttttaacg caggtttttt cataaaattt attgtgtaaa
    16681 atatttaaat aaatttacat ttgaaagtta agaggtaa
//
LOCUS       DAORVQ010000053        16569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1493_length_16569_cov_4.731803, whole genome shotgun sequence.
ACCESSION   DAORVQ010000053 DAORVQ010000000
VERSION     DAORVQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_16569_cov_4.731803"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..753
                     /locus_tag="PLX80_06140"
     CDS             28..753
                     /locus_tag="PLX80_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY20411.1"
                     /translation="MINSNDFRSDKVKEKLGFSPNELNNVILSRKNDFIKPRSSNQLE
                     YFNKVKDNDIVFAIGPAGTGKTYLAVAFAVSALKNKEINKIVLARPAVEAGESLGFLP
                     GDLSEKVDPYLRPLYDAIEDMLPYEKLENYIEKNIIEVIPLAFMRGRTLSNSFVILDE
                     AQNSTTMQMKMFLTRLGPNSKAIITGDVTQIDLPRKEQSGLIQVEEILKNIDDISFVY
                     FEKSDVVRHKLVKDIINAYEKLQ"
     gene            complement(755..2191)
                     /gene="rsmB"
                     /locus_tag="PLX80_06145"
     CDS             complement(755..2191)
                     /gene="rsmB"
                     /locus_tag="PLX80_06145"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQY20412.1"
                     /translation="MENQKLKSLTPKDQQNGSSSLEKIYNKNLFEDARSCSVKILCRC
                     ERTDSYLEKLIDAELRNETLNDFDKALLNEISHGVIRWMRRLDWFLNGFYRGNYEKCL
                     PEVRNALRVALYQILFLNKIPYSAAVNEAVEFVKRIHGDKHAGVVNGLLRTIIRTLEN
                     LVWPTREIDEVNYLGIIQSHPNWMVRRWINRFGFDDAVKLCEENNRRPVMALRVNTLK
                     ISVSDFENYLKEKNLFYKKGNYLDNFFSTKTMSKLFTDEYFKQGFFSIQDESAGLVSK
                     LLDPKKEELILDLCSAPGGKTSHISELMGNEGKIIAVEKYLSRLEIMRTNLNRLGVKN
                     VETFHDNVCEPETTELKESLIGKADKILIDAPCSGLGVLSKKPDIKWKREPEDIINLQ
                     KIQLEILESSVKYLKDSGVILYSTCTTEKEENIDVVNLFLKDNPEFKIENASAFVDNS
                     VVNNDGCIELFPHKNKTDGAFSVRLKKN"
     gene            complement(2351..4354)
                     /locus_tag="PLX80_06150"
     CDS             complement(2351..4354)
                     /locus_tag="PLX80_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HQY20413.1"
                     /translation="MTETKDLIKGINDFFSETGILSEHFENYEYRDSQETMADEILKT
                     LEDKKHLFIEAPTGVGKSFAYLVPAIYFAKKNEKKVIVSTYTINLQEQLLHKDIPFLQ
                     KVLPLEFKAGILKGRNNYLCPRRLAKAMETSNTLFETEEQLNLERLYRWSKETIDGTR
                     SDINFPLNDNVWSSVCSERGICTIKTCGSENTKCFYQRAKKELADSDVIIVNHHLFFT
                     LFDGVSEEREGYLYNNDFVIFDEAHTVEQVATDHISPSLSREQMKYHLLRLYNQNKKK
                     GFLLTLPSLHIQMKIQNLLDLNQQFFYSLRKNLFDNDKSEYKKLTSRIYEKNVAENIL
                     KPELDNLLNDLRALRPACKDDSQENELNEYIIRLSEFSYVLEDFIGQKKNEKEKDFVY
                     WVELSSMKPDSNVSICSSPVDVSEYFRKNIFRENNSTILTSATLTINSNFEYFRKRLG
                     ADNLNEVKLNSPFDFYKQVRILIPKEIPEPGKNNSGVYAELLKNWICHFTLMSKGKAL
                     VLFTNGTLLRKIGNEISEELNSHDITLLMQVPGVSRNSLLSKFKSDINSVLFGLDSFW
                     MGVDVPGDALSNLIITRLPFQVPDHPVVQAKMEFIEKHGGNSFFEYSLPEAILKFRQG
                     VGRLIRHRSDKGIIVILDSRILTKSYGKYFINSLDECEIEIVE"
     gene            4425..4967
                     /locus_tag="PLX80_06155"
     CDS             4425..4967
                     /locus_tag="PLX80_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQY20414.1"
                     /translation="MRKLTHSEIEKSRKKPEEILNYKRNPVYVLCDNIRSIFNVGAIF
                     RTSDAAFVEKIYLTGYTPYPPRKEIEKVALGATETVPWEYYKDPVEIVLNLKKENIKI
                     AVLEITDEQNLIWNVKPADFPLCLVLGNEITGVSKEIIDLADMSFEIPMLGMKQSLNV
                     SVAYGVAVFEMVKKIQFNKE"
     gene            5058..5561
                     /locus_tag="PLX80_06160"
     CDS             5058..5561
                     /locus_tag="PLX80_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20415.1"
                     /translation="MNKIIAVLTIIITFTGTLSAQYKGYDDGKTKYEPSSNNMILGFI
                     NPNNFTMNHSFNVSMVNTGTGNVSLTSYINSMNYRISDKLNISADVRFQYSPYASSIY
                     GPDAATNLQNDLSGIALSRASLNYRISDNSYINVEFRQIDQSDYLNNYNNNYYNPFIT
                     NSRYAGY"
     gene            5647..6117
                     /locus_tag="PLX80_06165"
     CDS             5647..6117
                     /locus_tag="PLX80_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HQY20416.1"
                     /translation="MNKSFLNYILNGVIIIFLIFVGYLGFSLINNSVKTEKKIDVISD
                     TSKNLTNQPNLTIQLDVQNGTEENGIAGLFTEYLRTNGVDVVESGNFKANDQEKTIVI
                     DRSGDGRKANKVASILGINSRNIIQQINNSLYLDATVVIGKDFKELKPFSEQRK"
     gene            6121..6486
                     /gene="rsfS"
                     /locus_tag="PLX80_06170"
     CDS             6121..6486
                     /gene="rsfS"
                     /locus_tag="PLX80_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQY20417.1"
                     /translation="MESKKLAEKLGDLMLQKNGKDILIMDLRSLTTVTDFFVICSASS
                     DVQVKAIAEYIKDETGKLDEGPWHKEGFTNFKWVLLDFVNVVVHIFLEDSRKFYDLEG
                     LWADAPVTIVSDEELNEKT"
     gene            6473..7447
                     /gene="pdxA"
                     /locus_tag="PLX80_06175"
     CDS             6473..7447
                     /gene="pdxA"
                     /locus_tag="PLX80_06175"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQY20418.1"
                     /translation="MKKRKQKILLTTGDPNGIGPEIILKIFSDNKFTSMFDLFISGNK
                     KIFDIYSDILNIKTISPDRLINLKLPKGFKLTPGKIQKSAGEFSGDSINQSVRLCMEK
                     KFDAVVTMPISKESLNLGNYKYPGHTEMLKDITKSEDAVMILYSKKFSVAMITGHIPV
                     SGISKLINEKNSEQSISEKIITVNNSLVADLGKKSPRIGILALNPHAGDGGLLGTEEY
                     EILSPVIEELNSVGFNARGPFAADAYFANRIYKKFDITIAMYHDQGLIPFKMISFGMG
                     VNYTAGLNIVRTSPDHGTAFDIAGLGRAEVESTKQAILLAAEISKRRN"
     gene            complement(7452..8867)
                     /locus_tag="PLX80_06180"
     CDS             complement(7452..8867)
                     /locus_tag="PLX80_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY20419.1"
                     /translation="MKDYDDLDFFGEEDSENEKNRNRKNEELEKILKLEEEAKYNFDA
                     ENLEEIINFYFDAFEFEKALSYVNVLLDIYPYSTEAWHKKALILDSLGKFEEAIEYFE
                     KAILLDPTDIEIYINKGITLDNLDKYDEALNCFMRAIEMDPYNSDALFNMGLTYEKLE
                     KFTEAAECFENVLKINNDHKDAYYELGYCFDFLDRLSDSISSYDKHLKHSPLNYTAWY
                     NRGIVLNRTGKFLKAIESYEMCLALKNNFPSAWYNCGNAYASVGRLYKAIDCYNSALN
                     LKKDDPFTLHNLGNAYEELGDFVTAIHYFTEAINADNYNYESYYGRGNCYFNFGNYEK
                     AIEDFNSAINLCKDYSEIWYLKADSEHALGRIEDAVKSYSVVVKLDDKNYDAWYDYGC
                     ALFDLKNLNLSLNVFDIAIKLKPDWHDPYYEKAKIYFMKEDPASGIEMIEKAFELNPL
                     DRFEYNIEKDWKKLLEFLVNR"
     gene            9126..9977
                     /locus_tag="PLX80_06185"
     CDS             9126..9977
                     /locus_tag="PLX80_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY20420.1"
                     /translation="MTDKFQSYLPKNHKFKNILYKKDGYKSTITINREKKLNCLDLLT
                     INELTLAFKDSSWDDQIAVIVLTGTGKRAFSTGADLDEQEKYFLKNPDDYYKWMYEFI
                     QLHEVMRNVGKPTIARLNGMVVGGGNELNMSCDLAIASDHIIIKQVGAARGSVAAAGA
                     TQFLPLIVGDRRAREILFLCEDIPVMKALDWGLVNQVVPFEELDKAVDILAEKLFNKL
                     PECLRFTKQQINFWRDLSWHTTIGSARDWLSIHNLSEEVTEGISAFNEKRKINYKKVR
                     TKGKKKQ"
     gene            complement(10002..11555)
                     /locus_tag="PLX80_06190"
     CDS             complement(10002..11555)
                     /locus_tag="PLX80_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HQY20421.1"
                     /translation="MNKTIAAIDIGTNSFHLVIAEIDSKNRFKILTKSKEVVRLGNSS
                     NDMKYISEESILRGVEVLNRFKLICESYKAEIIAVATSATREALNQNEFKLRVFVKTG
                     IEINIVSGYEEARLIYLGIMQSLEIFDKKILMVDIGGGSTEFLVGHSGEVKYANSIKI
                     GAVRLTKKFSLDKKISKNDLKDSKLFTRNILNQVVRSLENENYEMVVGSSGTIINIGN
                     IIRSDNQNISEAEVSLNGYIIKKKPLSSAFEKVYAAETYSDRSKIKGLDPKRIDIITA
                     GCIILEQIFSEIKIDKMTISSYALREGIIINYIQQYRSGKDFVSFKNVRYNSVIHLCE
                     LTNFDREHSFKVMENAAIIFDSVAEKFGLDGNDKEYLESACLLHDIGYHISAASHHKH
                     SYYIIRNADLMGFNDKEIEIIANTARYHRKSHPKPKHESFSKLDLESQDKVKKLSGIL
                     RIADAFDRSHNSVIKKFDSLLENKTLNLNIISCKSDPSLEIWGAGQRKDLFENSFDLK
                     VKINLKTTS"
     gene            complement(11580..12701)
                     /locus_tag="PLX80_06195"
     CDS             complement(11580..12701)
                     /locus_tag="PLX80_06195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20422.1"
                     /translation="MLKFFLKSSGKFDPKILSPEKVKNILIVRQHNQLGDMLCSVPLF
                     AAIRKKYPEAHITLVASPINYEILFSDINPYINKVITYRKAPLKKLIEFYKELKNHDY
                     QIGIVPSTVSLSRTSHLINYFSGAKVRVGVKSIDGKLNDMEYLLNVKSDFEWDTKKLH
                     QVDRNLDVGKQIGCVLSESEKSEVMIHLSNDEISFADEYVKNNFPDVNKIIIGFHPGA
                     GKTQNRWDKENFIALIKLLHHKYKCYLMMTSGTIDKEITHHIKSELNKSGIEIIILEN
                     TPIRKLSAVLKKLDLYITNDTGTLHVAAGVDANIISLFGPTHGYEWAPIGENKIYIQS
                     PTNEINDITVENVFEKAENLIESISYNSEKKNLISQDHD"
     gene            complement(12709..12891)
                     /locus_tag="PLX80_06200"
     CDS             complement(12709..12891)
                     /locus_tag="PLX80_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20423.1"
                     /translation="MIKTIIIFIIIFILLSVVKTFFKMSRLKSGSNKNVNTGNKKSDS
                     KKDDGNIVDAKYEEIN"
     gene            complement(12894..13556)
                     /locus_tag="PLX80_06205"
     CDS             complement(12894..13556)
                     /locus_tag="PLX80_06205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQY20424.1"
                     /translation="MSSEQKIRYIAIEGVIGAGKTSLAKKLSERLNAKLILENFEDNP
                     FLEKFYHDPSGYAFHTQMYFLMSRYKQLQEVKQTDMFHDFFIADYIFEKDKIFAYLNL
                     QDDELKLYEKIVTLIEKNIVVPDLIIYLQSTLERLMKNIKHRDRDIEKEIKEDYIKDL
                     NEGYNHFFFRYKSTKVLIVNSAEIDFVHNEKDFENLISEILKPEHSAIEFYNPGSANI
                     AH"
     gene            complement(13562..14074)
                     /gene="folK"
                     /locus_tag="PLX80_06210"
     CDS             complement(13562..14074)
                     /gene="folK"
                     /locus_tag="PLX80_06210"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQY20425.1"
                     /translation="MKTKNIAYLGLGSNIGERISYIQRVITELNDSRNINILKESSIF
                     ETEPWGNIDQDNYLNSVLKIETDLGPMELLLELKSLEKKIGRSESKKWSEREIDIDIL
                     FYGDLVIQNENIKIPHSLIEERKFVLVPLAEIAPEFIHPVLNKSAVQLLSDSKDNLKV
                     SPYCILKSEA"
     gene            complement(14078..14440)
                     /gene="folB"
                     /locus_tag="PLX80_06215"
     CDS             complement(14078..14440)
                     /gene="folB"
                     /locus_tag="PLX80_06215"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQY20426.1"
                     /translation="MTKIRINNAKFFSYHGVLDYEKEYGNEFEVDIEMECELPDLSLS
                     DDLSLTVNYLEVYNMVRVIFMENKYDLIETLNIQICKMILKNFPLVSSVEVKIRKPNA
                     PLGIIDSVEIVNNLKRSV"
     gene            complement(14451..15485)
                     /gene="sucD"
                     /locus_tag="PLX80_06220"
     CDS             complement(14451..15485)
                     /gene="sucD"
                     /locus_tag="PLX80_06220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQY20427.1"
                     /translation="MSILVNSNTKLVVQGITGSEGTFHTNQMMEYGTNVVAGVTPGKG
                     GTSFKSDKNKSVPIFNSVKEAVKKTKADASVIFVPAPFAADAIMEAADAGINVIVCIT
                     EGIPTNDMIKAYDYVKNKNTDSHSVRFIGPNCPGIITPDECKIGIMPGFIHKKGKVGL
                     ISRSGTLTYEAVWQLTNLGIGQSTCIGIGGDPVIGTRFLDAVKLFNEDKNTEAILMIG
                     EIGGNAEEEAAYYIKANVKKPVIGFIAGRTAPPGRRMGHAGAIILGGKGTADEKIKIM
                     KSCGISIADSPADIGVTVKKAIEKSKKSKSAKSVSEKKPLVKKNNSAKTAVKKQTVKK
                     STSKKKVSLK"
     gene            complement(15494..16120)
                     /locus_tag="PLX80_06225"
     CDS             complement(15494..16120)
                     /locus_tag="PLX80_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20428.1"
                     /translation="MTVFLSCDKNEENQYTPPEDEISVDKEKELREREEFERLKRLQD
                     GISDSSGLSSDSLLNAVDSAKIVSDSLAKIRTQEEKKRMVQKEKELNKRLDNPRIAVT
                     DYLEFLKRGTSEGGDFENNMKKADAQWQNGNLKRFTANYKNTKKIVTLDEPKVVSQDG
                     NNAEVEVRIKKTDIVNDKEVETEMTVKYFLIADSNGKWKIKNNTVIKK"
BASE COUNT         5399 a         2725 c         2582 g         5863 t
ORIGIN      
        1 cttaaggatg ttaatcttgt attggatctg atcaattcaa atgatttcag atctgataaa
       61 gttaaagaaa aattaggatt ttcaccaaat gaattaaata atgtaatttt atcaaggaaa
      121 aatgatttca taaagccaag atcttccaat cagcttgaat attttaataa agtaaaagat
      181 aatgatattg tctttgcaat tggacctgcc ggtaccggaa aaacttatct tgcagttgct
      241 tttgctgtat cagcattgaa aaataaagag atcaataaaa ttgtacttgc cagacctgct
      301 gtcgaagctg gtgaaagtct tggattttta ccgggtgatc tgtctgagaa agttgatcct
      361 tacttaagac ctttgtacga tgcaatagaa gatatgctgc cttatgaaaa acttgaaaat
      421 tatattgaaa aaaatattat tgaagtaatt ccactggcat ttatgagagg taggacttta
      481 agtaattcat ttgtgattct tgatgaagca cagaattcga ctacaatgca gatgaagatg
      541 tttcttacaa gattaggacc caattctaaa gcaattatta caggagatgt aactcagata
      601 gacctgcccc ggaaagaaca atcgggattg atccaggtgg aagagatctt aaaaaatatc
      661 gatgatatct ctttcgttta ttttgaaaaa tctgatgttg taagacataa acttgtgaag
      721 gatattatta acgcttatga aaagcttcag taacttaatt cttttttaat cttacggaaa
      781 acgctccatc agttttattt ttatgtggaa acaattctat acagccatca ttattaacaa
      841 ctgagttatc aacaaaagca gatgcatttt cgattttgaa ttcaggatta tccttgagaa
      901 acagatttac aacatcaatg ttttcttctt tttcagtagt acaggtgctg tataatatta
      961 ctccactgtc cttaagatat ttgactgagc tttcaagaat ttcaagctga attttttgca
     1021 gattaataat gtcttcaggt tcgcgtttcc attttatatc aggctttttg gaaagtacac
     1081 ctaatccaga acaaggtgca tctataagaa tcttatcagc ttttccaata agagattctt
     1141 tcagttcagt agtttcaggt tcacaaacat tatcatgaaa tgtctctaca ttttttacac
     1201 caagtctgtt aaggttggtt ctcattattt caagccgcga taaatatttt tccactgcga
     1261 tgatcttacc ttcatttccc attagttcgg atatgtggga agtctttccg cccggtgcag
     1321 agcaaagatc aagtatcaac tcttcttttt tcggatccaa taattttgaa actaatccag
     1381 cgctttcatc ctgaattgag aaaaaccctt gcttaaaata ttcatctgtg aaaagttttg
     1441 acattgtctt ggtagaaaaa aagttatcaa gataattacc ttttttataa aaaaggtttt
     1501 tctcttttag ataattttca aaatcagata ctgatatttt tagagtattt actctcaaag
     1561 ccattacagg tcttctgttg ttttcttcgc ataattttac agcatcatca aatccaaatc
     1621 tgtttatcca tcttcttacc atccagtttg gatgagattg aattattccg agatagttca
     1681 cttcatcaat ttctctcgta ggccagacaa gattttccag agttcttata attgtcctta
     1741 ataaaccatt tactacgcct gcatgcttat caccatgtat tctttttaca aattccacgg
     1801 cttcatttac tgctgcagaa tatggaattt tattaagaaa taatatctga taaagagcta
     1861 ctctgagtgc atttctgact tcaggaagac atttctcata attacctctg tagaagccat
     1921 ttaaaaacca gtccagtcgt ctcatccatc taataacgcc atgtgaaatt tcattaagca
     1981 gtgctttatc aaaatcatta agagtctcat tccttaattc tgcgtcaatt aatttttcaa
     2041 gatatgagtc agttctttca catctgcaaa gtatttttac agagcaggag cgggcgtctt
     2101 caaacagatt cttattgtaa attttttcaa gagatgatga accgttttgc tggtcctttg
     2161 gtgtaaggga ttttagtttt tgattttcca tttgttaaat cttataatat ccaaggtgct
     2221 ttggtttcta aaggtaacaa aataaatatt agaatagata tttgaaatgt attaatgaaa
     2281 taaactcaga gatgtatttg agatggggtt gagatgttaa agaaaggttt atatttaaaa
     2341 atagataata ttattcaacg atctcaattt cacattcatc aagtgaattg ataaaatatt
     2401 taccataaga ttttgttaaa attctgctgt ctaaaatcac aattattcct ttatcacttc
     2461 tgtgtcttat aagccttcct actccttgtc ggaatttcaa aatagcttca ggaagtgagt
     2521 attcaaaaaa gctgttacct ccatgttttt caatgaattc catttttgcc tgtacaaccg
     2581 gatgatcggg tacctgaaaa ggcagccgtg ttataatcag attacttaaa gcgtcacccg
     2641 gtacatccac tcccatccag aaactatcca aaccaaacaa cactgaatta atatcagatt
     2701 taaatttact taacaaggaa tttcttgaaa ctccgggtac ttgcataaga agtgtaatat
     2761 catgtgagtt tagctcttcg gaaatttcat taccgatctt tcttaataat gtaccgttcg
     2821 taaaaagtac aagcgctttc cctttcgaca ttaaagtaaa atgacatatc cagttcttta
     2881 aaagttctgc ataaacaccg ctgttatttt tcccgggttc aggaatttct ttcggtatca
     2941 gaattctaac ctgtttataa aaatcaaacg gtgaattgag ttttacttca ttcagattat
     3001 ccgcacccaa tcgctttctg aaatattcaa aattactgtt tattgttaaa gtagcacttg
     3061 taagaatagt agagttattt tcacgaaaaa tattttttct gaaatattcg gatacgtcaa
     3121 caggagaaga gcagatggat acatttgaat caggtttcat agaagataat tcaacccagt
     3181 aaacaaagtc tttttccttt tcattttttt tctgtccgat aaaatcttcc agtacataac
     3241 tgaattccga taatcttata atatattcat tcagttcatt ttcctgagaa tcatctttgc
     3301 atgcaggtct tagagctctg agatcattaa gtaaattatc aagttcaggt tttaagatat
     3361 tctcagcaac atttttttca tatatccggg aagtaagttt tttatattcg cttttatcat
     3421 tatcaaataa attttttctt aatgaataaa aaaactgctg attcagatca agaagatttt
     3481 gaattttcat ttgaatatga agggagggta atgtcagcag gaaacccttt tttttattct
     3541 gattatataa tcttagcaga tggtatttca tttgttccct ggacagggat ggagagatat
     3601 gatctgttgc aacctgttcg acagtatgag cttcatcaaa tatcacaaaa tcattattat
     3661 aaagataacc ttcacgctct tcagatacgc cgtcaaacaa tgtaaagaac aaatgatgat
     3721 tgacaattat cacatcagaa tctgctaatt cttttttagc acgctgataa aagcatttag
     3781 tattttcact gccgcaagtt ttaattgtac agatacctct ttcggaacaa actgaactcc
     3841 atacattatc atttaaagga aaattaatat cagagcgggt tccgtctatg gtttctttgg
     3901 accatctgta aagtctttca agattaagct gctcttcagt ttcgaacaaa gtattggaag
     3961 tttccatagc tttggcaagt cttctcgggc agagataatt atttcttccc tttagaattc
     4021 ctgctttaaa ctcaagcggt aatactttct gaagaaatgg aatgtctttg tgaaggagtt
     4081 gctcctgcag attaatagta taggtcgaaa caattacttt cttttcattt ttctttgcaa
     4141 aataaattgc aggtacaagg tatgcaaaac tttttccaac tcctgtagga gcttcaatga
     4201 acagatgttt tttgtcttca agtgttttta gaatttcatc agccatagtc tcctgagaat
     4261 ctctgtactc gtaattttca aaatgctctg agagaattcc tgtttcagag aaaaaatcat
     4321 ttatgccttt tattaagtct ttggtttccg tcaattataa tgaattgttt attaaacaaa
     4381 actttgaaat ctttacaagt tatttattga aatactaata taaaatgcga aaacttactc
     4441 attccgaaat tgagaaatcc agaaaaaagc ctgaagaaat tttaaattac aaaaggaatc
     4501 cagtatatgt actttgcgat aatatcagaa gtatatttaa tgtgggggca attttcagaa
     4561 cttcagatgc tgcatttgtt gaaaaaattt atttaacagg atatacgcct taccctccca
     4621 gaaaagaaat agaaaaagta gctttgggtg ctactgaaac agttccctgg gaatattata
     4681 aagatccggt ggaaattgtt ttaaatctca aaaaggaaaa tataaaaatt gcagtattag
     4741 aaataacaga tgaacagaat ctgatttgga atgttaaacc tgcagatttc ccgctttgtc
     4801 ttgtacttgg taatgagatc acaggagtgt ctaaagaaat tattgatctt gcagatatgt
     4861 catttgaaat tccaatgctg ggaatgaaac aatctttgaa tgtatctgta gcatacgggg
     4921 ttgccgtgtt tgaaatggtc aaaaagattc aattcaacaa agaataacta atttatcccg
     4981 aaaatattta cagatattgc atttgaattg tcattgacat ttttatctat aaacaggaat
     5041 tttaaaaaaa aaatattatg aataaaatca tagcagttct aactataata ataacattta
     5101 ccggtacatt atctgctcaa tataaagggt atgatgacgg aaaaacgaaa tacgaaccaa
     5161 gttcaaataa tatgatattg ggatttatta atcctaataa ttttacaatg aatcattctt
     5221 ttaatgtttc gatggtaaat acaggcacag ggaatgtttc acttacttca tatataaatt
     5281 ctatgaatta caggatctcc gacaaactga atatcagcgc agatgtgaga tttcaatatt
     5341 caccttacgc ttcttcaatt tacggacctg atgccgccac gaatcttcag aatgatcttt
     5401 caggaatagc attatccaga gcttcactca attacagaat ttctgataat tcatatatta
     5461 atgttgaatt cagacaaatt gatcaaagtg attatctgaa taattataat aataattatt
     5521 ataatccatt tattacaaat tcaagatatg cgggttattg atactggagt atcattacag
     5581 atattaattt ttattatttc aaaccgaaag taattaattg ctttcggttt tataatttat
     5641 atttaattga ataaaagttt tttaaattat attctaaatg gtgttataat catttttctt
     5701 atttttgtgg ggtatctggg attttcactc ataaataatt ctgtcaaaac agaaaaaaaa
     5761 attgatgtaa tttctgatac ttcaaaaaat ctgacaaatc aacctaatct cacaattcag
     5821 cttgatgtac agaacggaac tgaagaaaat ggcatcgcag gattatttac tgaatacctc
     5881 agaactaacg gagtggatgt tgtagagtca ggtaatttca aagctaatga tcaggagaaa
     5941 acaattgtga tagacagaag cggtgatgga agaaaggcaa ataaggtagc atctatactt
     6001 ggaataaaca gcaggaatat tattcagcaa attaacaata gtctgtatct ggatgctact
     6061 gtagtaatag gaaaagattt taaagaactc aaaccattta gtgaacaaag aaaataaaat
     6121 atggaatcaa aaaaattagc agaaaaactt ggtgatctga tgcttcagaa aaacggaaaa
     6181 gatattttaa taatggatct tcgaagtctg acaacggtta cagatttttt tgtgatctgt
     6241 tcggcatcat cagatgttca ggtaaaagca attgctgaat atataaaaga tgagacagga
     6301 aagttagatg aaggaccatg gcacaaagaa ggttttacaa atttcaaatg ggttttactg
     6361 gattttgtga atgtagtagt acacatattt ctcgaagatt cccgtaaatt ttatgatctg
     6421 gaggggcttt gggcagatgc accggtaaca attgtcagtg atgaagaatt aaatgaaaaa
     6481 acgtaaacag aaaatattac ttactaccgg agatccaaac ggaataggtc cggagatcat
     6541 actgaaaatt tttagtgata ataagtttac ttcaatgttt gatcttttta tctcaggaaa
     6601 taaaaaaatt ttcgacatct actcggatat tcttaacata aaaaccattt cacccgaccg
     6661 cttaattaat ctaaaattac caaaaggatt taaactgacg cctggtaaga tacagaaatc
     6721 tgcaggtgaa ttttcaggtg attcgataaa tcaatcagtc agactgtgta tggagaaaaa
     6781 gtttgatgca gttgtaacta tgccaatctc taaggaatct ctgaatctcg gaaattataa
     6841 atatccgggt catacagaaa tgctgaagga tataacaaaa tctgaagatg cagtaatgat
     6901 cttatattct aaaaaatttt cagttgcaat gatcactggt catattcctg taagtggaat
     6961 ttccaaatta attaacgaaa aaaattcaga acaatctatt tcagaaaaaa tcattactgt
     7021 taacaattct cttgttgcag accttggcaa aaaatcgcct cgaattggaa tcctcgcttt
     7081 aaatcctcat gcaggagacg gtggtttgtt aggtactgaa gaatatgaga ttttatcgcc
     7141 tgtcattgag gagctgaatt ctgtgggttt taatgctcgt ggaccttttg cagcggatgc
     7201 atattttgca aaccggattt ataaaaaatt cgatattaca attgcaatgt atcatgatca
     7261 gggattgata ccttttaaaa tgatctcttt tggaatggga gtaaattata cagcaggatt
     7321 aaatattgta aggacttcac ctgaccatgg taccgcattt gatattgcgg gacttggtag
     7381 agcagaagta gaaagtacaa agcaggcaat tttattggct gctgaaatat caaagagaag
     7441 aaattaagag gtcatctgtt tactaaaaac tccagaagtt ttttccagtc tttttctatg
     7501 ttatattcaa atctgtcaag tggatttaat tcaaatgctt tctctatcat ttctataccg
     7561 gaagcaggat cttctttcat aaaataaatt ttagcctttt cataataagg atcatgccag
     7621 tcaggcttta gttttatagc tatgtcaaat acattaagag acagattgag attttttaaa
     7681 tcaaataagg cgcatccgta atcataccag gcatcataat ttttatcatc aagttttaca
     7741 accacagaat aacttttgac tgcatcctct atcctgccca gtgcatgttc tgaatcagct
     7801 ttaagatacc atatttcaga ataatcctta cagagattta ttgcagaatt aaaatcttca
     7861 attgcttttt cataatttcc gaaattgaaa taacagttgc ctcttccgta gtaagattcg
     7921 taattataat tatcagcatt tatagcttca gtaaagtaat gtattgcagt aacaaaatca
     7981 ccgagttctt cataagcatt tcccagatta tgcagtgtaa aaggatcatc tttttttaaa
     8041 ttcaaagcac tgttataaca atcaatcgct ttgtataatc tgccaactga agcatatgca
     8101 tttccgcagt tataccatgc agaaggaaaa ttatttttta aagcaagaca catttcataa
     8161 ctttcaatgg ctttcaaaaa tttaccggtt ctgtttaaaa caatacccct gttataccat
     8221 gcagtataat ttagaggaga atgtttaaga tgcttatcat atgaacttat ggaatcactt
     8281 agtctgtcaa gaaagtcaaa acaataaccg agttcataat atgcatcttt atgatcatta
     8341 tttattttaa gaacattctc aaaacattca gcagcttcag taaacttttc aagtttctca
     8401 tatgtaagac ccatgttaaa caaagcatca gaattataag gatccatttc aatcgctctc
     8461 ataaaacagt tcagagcttc atcatattta tcaagattat ccagtgttat tcccttattt
     8521 atataaattt caatgtctgt aggatccagc aggattgctt tttcaaaata ttcaattgct
     8581 tcttcgaatt ttccgagact atccaaaatc agagcttttt tatgccaggc ttctgttgaa
     8641 tagggataga tatcgagaag tacattaacg tagctcaaag ctttttcaaa ttcaaaagca
     8701 tcaaaataaa aattaatgat ttcttctaga ttttctgcgt caaaattata tttagcttct
     8761 tcctcaagtt ttaatatttt ttctaactct tcattttttc tgttcctgtt tttctcattt
     8821 tcagagtctt cttcaccgaa aaaatccaaa tcatcataat ctttcaactt aacctcctgg
     8881 ttaaaattaa ttcaagaaaa aactttttat gaaattatat aaaagtcatt attaagtcaa
     8941 gtaattatta gcagagggag aaatttttgg ataaaatttt ttaaattttt ttgaagttaa
     9001 actttgaaaa tatttttgta tgaattttaa atatcaggtc attaatttaa atttttggat
     9061 aaattcaatt tgctattatt tatatattga taaaaatttt tttaaaaaat taaataaacg
     9121 taaaattgac tgataaattt caatcgtatt tacccaaaaa tcacaagttc aaaaacatcc
     9181 tttataaaaa ggacggatat aaatccacta ttactattaa cagagaaaaa aaattaaact
     9241 gtctggatct gcttacaatt aatgagctga cactggcatt caaggattct tcatgggatg
     9301 atcagatcgc tgttatagtt cttacaggca caggaaaaag agctttctca accggagcag
     9361 atcttgatga acaggaaaaa tattttttga aaaatccgga tgactattac aaatggatgt
     9421 atgaatttat acagcttcat gaagtaatga gaaatgtagg aaagcccact atagcaaggc
     9481 tgaacggaat ggttgtcggt ggcggcaatg aacttaatat gagttgtgat ctggcaattg
     9541 cttcagatca tataattata aaacaggtag gggcagccag aggcagtgtt gcggctgcag
     9601 gggcaacaca atttctgccg ttgattgtag gtgacagaag agcacgggag atactttttc
     9661 tttgtgaaga tataccggtg atgaaggcac ttgactgggg gcttgtaaat caggttgttc
     9721 cgtttgaaga attagataaa gctgtagata ttttagcaga gaaattattt aacaaacttc
     9781 ccgaatgcct cagatttaca aagcagcaga ttaatttctg gagagacctt tcatggcata
     9841 ctactattgg aagtgcaaga gactggcttt ctattcataa tttatctgaa gaagtaactg
     9901 aaggaatttc agcttttaac gaaaagagaa agataaatta caaaaaggta agaactaaag
     9961 gaaagaagaa acagtaacct gagatttttg ttggagacag gtcaggaagt ggtttttaaa
    10021 ttgatcttca ctttcagatc aaaactattt tcgaacagat cttttcgctg cccggctccc
    10081 catatctcaa gtgaaggatc tgatttgcag gatatgatat tgaggttaag agttttgttt
    10141 tcaagaagtg aatcaaactt ttttattaca ctattatgac ttctgtcaaa tgcatcagct
    10201 atccgaagta tcccggaaag tttttttact ttatcctggc tttctaaatc aagcttggaa
    10261 aatgattcat gcttgggttt tggatgagac tttcggtgat acctggcagt gttagcgatt
    10321 atctcaatct ctttgtcatt aaatcccatt agatctgcat tccttataat gtagtaggaa
    10381 tgtttgtgat ggctcgctgc tgagatatga taaccaatgt catgaagcag acatgcagat
    10441 tcaagatatt ctttgtcatt tccatcaaga ccaaattttt cagctactga atcaaaaatt
    10501 attgcagcat tttccatgac tttaaatgaa tgctccctgt cgaaatttgt taattcacat
    10561 aaatgaataa cgctgttata cctgacattt ttaaaactta caaaatcttt tccgcttctg
    10621 tattgctgaa tataatttat tataatacct tctctgagag catagcttga gatggtcatt
    10681 ttatcgatct taatttcaga gaagatctgc tcaagtataa tacagcctgc ggtaataata
    10741 tcaattctct ttggatccag tccttttatc tttgatctgt ctgaatatgt ttctgcagca
    10801 tatacttttt caaaagcact gcttaaaggt tttttcttta taatgtatcc gttcaagctt
    10861 acttctgctt cagatatatt ttgattatct gatctgataa tattgccaat gtttataatt
    10921 gtaccggaag atccaacgac catttcgtaa ttctcatttt ccagagacct gacaacctga
    10981 ttaagaatat tccgggtaaa taatttagaa tccttcagat cattcttact gatcttttta
    11041 tcgagtgaaa atttctttgt aagtctgaca gctcctatct ttatactgtt tgcatatttt
    11101 acctctcctg aatgacctac aagaaattcc gtactgccgc cgccgatgtc taccattaat
    11161 atttttttat caaagatctc caatgactgc attataccaa gatagatcag tcttgcttct
    11221 tcatatccgg atacaatatt gatctcaatt ccggttttga caaagaccct gagtttaaat
    11281 tcattttgat tcaatgcttc acgggtagca ctcgtagcaa cagcaatgat ctctgcttta
    11341 tatgattcac aaataagttt aaacctgttt aaaacttcaa cacctctgag tatggattct
    11401 tcagagatat atttcatatc gtttgaactg ttaccaaggc gtactacttc ttttgacttt
    11461 gtgagaattt tgaatctgtt tttgctgtct atctcagcaa taacaagatg aaatgaattt
    11521 gtaccaatat ctattgcggc aatagttttg ttcaaatttt ataaacgaat aataaaattt
    11581 caatcatgat cctgtgaaat cagatttttt ttctctgaat tataacttat ggactcaatt
    11641 aagttctcag ctttttcaaa aacattctca acggtaatat cattgatctc gtttgtagga
    11701 gactgaatat aaattttatt ttctccaatt ggagcccatt cataaccatg tgtcggacca
    11761 aataaagaaa ttatatttgc atctactccg gctgcaacat ggagagttcc cgtatcattt
    11821 gtaatataca gatcaagctt ttttaataca gcactgagtt ttcttatagg agtattttca
    11881 agaattataa tttcaatgcc tgatttattc agctcggatt taatatgatg agtgatctct
    11941 ttatctatag ttccggatgt catcattaaa tagcatttgt atttatgatg taacaattta
    12001 attaaagcaa tgaaattttc tttgtcccag cggttttgag tcttacctgc ccccggatga
    12061 aatccaatga tgatcttatt tacatcagga aaattatttt taacatattc atcagcaaat
    12121 gaaatctcat cattactaag atgtatcatg acctcacttt tttcagattc agaaagaacg
    12181 catccgatct gtttaccgac atcaagattt ctgtcaacct gatgcagctt tttagtatcc
    12241 cattcaaaat cagattttac attcagaaga tattccatat catttaattt accgtcaatg
    12301 cttttcacgc cgactcttac ttttgctcct gagaaataat taattaaatg agacgtacgt
    12361 gaaagtgaaa cagtagaagg gacgatccca atctgataat catgattctt taattcttta
    12421 taaaactcga tcagtttttt aagaggagct tttcggtatg ttataacttt gtttatataa
    12481 ggattaatat cactgaaaag tatttcataa ttaatagggg aagcaactaa tgtaatatga
    12541 gcttcaggat atttttttcg aatggctgca aacagtggta cagaacaaag catgtctcca
    12601 agctgattgt gttgtctgac tattaaaata ttttttactt tttccggaga gagtattttc
    12661 ggatcaaact tccctgatga tttcagaaaa aattttaaca tatcagattt aatttatttc
    12721 ttcatatttt gcatctacaa tattaccgtc atctttttta ctgtcagatt ttttattacc
    12781 ggtattaaca tttttgttgc ttccggattt aagacgcgac attttaaaaa aggtttttac
    12841 tactgaaagt aaaataaata ttataatgaa tattattatt gttttgatca tttttaatgc
    12901 gctatgtttg cagatcccgg attataaaat tctatagcag aatgttcagg ttttaaaatt
    12961 tcagatatca gattttcaaa atctttttca ttatgaacaa aatcaatttc ggcagaattt
    13021 actatcagaa ctttagtaga tttatatctg aagaaaaagt gattgtatcc ttcattaaga
    13081 tctttaatat aatcttcttt gatctctttc tctatatctc tgtccctgtg tttaatattt
    13141 ttcataagtc tttcaagagt agattgtaga taaatgataa gatcaggtac aacaatattt
    13201 ttttctatga gagttacgat cttttcgtat aatttcagtt catcatcctg aaggttgaga
    13261 taagcaaata tcttatcttt ctcaaaaata taatcagcaa taaaaaaatc atgaaacata
    13321 tcagtctgtt ttacttcctg aagctgtttg tatcggctca ttaagaaata catctgggtg
    13381 tgaaatgcat aacctgaagg atcatgataa aatttttcaa gaaacgggtt gtcttcgaaa
    13441 ttttcaagaa tcaattttgc atttagtctt tcagaaagtt tcttagccag ggaagttttt
    13501 cccgctccta tgacaccttc aattgcaata tatctgatct tctgttcaga tgacatattt
    13561 gtcaggcttc agattttaaa atacagtacg ggctgacctt gagattgtct tttgaatcgg
    13621 atagaagctg tacagcagat ttatttaaaa caggatgaat aaattccggc gctatttccg
    13681 caagaggtac taatacaaat tttctttctt caatgagcga atgaggaatc tttatatttt
    13741 cattctgaat gacaagatct ccgtaaaaaa gaatatcaat atcaatttct ctttcggacc
    13801 attttttgct ttcggatctg ccaatctttt tttcaagaga ctttaattcc aaaagtaatt
    13861 ccattggacc aagatcagtt tcaatcttca gaacggaatt aagataattg tcctgatcaa
    13921 tattacccca gggttcagtt tcaaagattg aagattcttt taaaatatta atgtttcgtg
    13981 aatcattcag ttctgtaata actctttgaa tatatgatat cctttcgccg atgtttgatc
    14041 ccagaccaag ataagcaata tttttagttt tcataaatta gactgatctt tttaaattat
    14101 taactatctc aacagaatca attatgccta acggggcatt tggttttctt atttttactt
    14161 caactgagct aaccagcgga aaatttttta atatcatttt acaaatttga atatttaaag
    14221 tctctatgag atcgtattta ttttccatga atattactct gaccatgttg taaacctcca
    14281 gatagttaac tgtcagactc agatcatcgc ttaaagaaag atcaggaagt tcacattcca
    14341 tttcgatgtc aacttcaaat tcatttccat attctttttc ataatctaaa actccgtgat
    14401 aagaaaaaaa ctttgcatta tttattctga tctttgtcaa tttacagaaa ttattttaaa
    14461 gatactttct tcttagatgt agattttttc acagtctgtt tttttactgc tgttttagct
    14521 gaattatttt ttttaaccaa aggttttttc tcagaaactg attttgcaga tttagatttt
    14581 ttagatttct caatagcttt ttttacagta actccgatat cagcaggtga gtctgcaatg
    14641 gaaattccgc agcttttcat aattttaatt ttttcatctg cagttccttt accgcctaag
    14701 attatagcac ctgcatgccc cattcttctt cccggaggag cagttcttcc ggcaataaat
    14761 ccaatcaccg gtttctttac atttgcttta atataatatg cagcttcttc ttctgcatta
    14821 ccgcctattt caccgatcat taaaattgct tctgtgtttt tatcttcgtt aaataattta
    14881 actgcatcaa ggaatctcgt gccaattaca ggatctccgc caatacctat acatgtagat
    14941 tgacctatac caagatttgt caactgccac acagcttcat aagttaatgt tccgcttctt
    15001 gaaataagtc cgacctttcc ttttttatga ataaatcccg gcatgatacc gatcttacat
    15061 tcgtcaggtg taatgatccc cggacagttt ggaccgataa atcttacact gtgtgaatca
    15121 gtattcttat tttttacata atcgtatgct tttatcatat catttgtagg aatgccttca
    15181 gtaatacaga caataacatt tatgccggca tcggctgctt ccattattgc atcggctgca
    15241 aaaggtgccg gtacaaaaat tacagatgcg tcagcttttg tttttttaac agcttctttt
    15301 actgaattaa aaataggtac ggatttgttt ttatcggact taaaactggt tcctcctttt
    15361 ccgggtgtta cacctgctac tacatttgta ccgtattcca tcatttgatt tgtgtggaat
    15421 gttccttcag aacctgtaat tccctgtact actaatttag tgttactgtt tactaatatg
    15481 ctcatttatt attttatttt ttaattactg tattattttt gattttccat tttccattgc
    15541 tgtcagcaat gaggaaatat tttactgtca tttcagtttc gacttcctta tcatttacaa
    15601 tatctgtttt tttaattctt acttcgacct cagcattgtt accatcttgt gaaactactt
    15661 taggttcatc aagtgtaaca attttttttg tgtttttata atttgctgtg aatcttttca
    15721 ggtttccgtt ctgccattga gcatctgcct ttttcatatt attttcaaaa tctccacctt
    15781 cgctggtgcc tcttttaaga aattccagat aatctgtaac agcaatccga ggattatcaa
    15841 gcctcttgtt caattctttt tctttttgaa ccattctttt tttttcttcc tgtgtcctga
    15901 tttttgcaag ggaatctgaa acaatttttg ctgaatctac tgcattcaat aatgaatcag
    15961 aacttaatcc tgaggaatcg gaaattccat cctgaagtct tttaagtctt tcaaactctt
    16021 ctctctcacg gagttccttt tctttatcta cagatatttc gtcttcggga ggagtatact
    16081 gattctcttc atttttatcg caggataaaa aaaccgtcat taatggaaat acaataatga
    16141 cagcgtaaaa aaaacactta actattttca tatttttgat tattatcaaa atagattttt
    16201 agagttttat attcaattta caaatgatac aaatcttaat aaatattaat taaattaatt
    16261 ttattcaaac aattatatat aaatctgatt aaccacaatt tataaattga caatggaaaa
    16321 aataataaat aactttataa aaatttaaag taaattaata caattaaata ctgctgaaaa
    16381 agagaaatct ttgtagtatt ttatttataa gattttataa aaaagtattt ttttttaaca
    16441 aattaaacaa ataggagaaa aaaatgagat caaaattatt gatttacatt ctaccggttt
    16501 tactagcaac attcctgatc tatggatatt ctactcagga tgatccaaga acagatgcat
    16561 caccgaagt
//
LOCUS       DAORVQ010000054        16385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1521_length_16385_cov_5.923025, whole genome shotgun sequence.
ACCESSION   DAORVQ010000054 DAORVQ010000000
VERSION     DAORVQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16385
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_16385_cov_5.923025"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /gene="lpxC"
                     /locus_tag="PLX80_06230"
     CDS             complement(<1..894)
                     /gene="lpxC"
                     /locus_tag="PLX80_06230"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQY20429.1"
                     /translation="MLEKQRTIKNEITLSGVGLHTGNKSNMTFKPAPENYGVKFKRTD
                     LPDSPEIPADIDHVIDISRGTTIAKDGAEVHTVEHVLASIMGCEIDNIIIELDSNEPP
                     IMDGSAKDYVETLKKADIVEQEAKRDYLVIEDTVHYHDDKTNVDIVALPLKDDFRISV
                     MIDYNNPALGVQHTGLFNLHKEFEKEFAPSRTFCFLSEIEYLQEQNLIKGGDLNNAIV
                     IVDKDASDEELERMKKKIGIEESIILGSNGILNNRELRFRNEPARHKLLDLIGDLALI
                     GAPVKGQILAARPGHKSNVEFT"
     gene            complement(914..1939)
                     /gene="lpxD"
                     /locus_tag="PLX80_06235"
     CDS             complement(914..1939)
                     /gene="lpxD"
                     /locus_tag="PLX80_06235"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQY20430.1"
                     /translation="MKLSEAANIINGKLEGDPEAEVTGIGKIETAEDHEITFISNPKY
                     EKYFDSTKAGCVIVSDGFEVTDNNAGKNISLIRVKDPYNSFLKLLEKFEIKEDITDIG
                     ISERCSIGTNVKTGEKVYAGINVVIENDCKIGDGTRIYANTYIGKNSILGKNCLIYPN
                     VTIYKDTVIGDNVIIHSGTVIGSDGFGFARQEDYTYKKIPQTGNVVIEDNVEIGSNTS
                     IDRATIGETRICKGVKIDNQIQIAHNVTIGENTVIAAQVGIAGSAKIGKRCMIGGQAG
                     IVGHIEICDDVIIGSSVGVSKSITVPGIYTGYRFRPYREDLKIEARIRNLDKLEERIK
                     KLESPGK"
     gene            complement(1940..2467)
                     /locus_tag="PLX80_06240"
     CDS             complement(1940..2467)
                     /locus_tag="PLX80_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQY20431.1"
                     /translation="MKIIRNITLAFTAVVFLMSAGLSAQTKVGYIDSKKIVESMQDSR
                     DAQQRLDNLVTQWQTELTQLQDSLKIIKDDYEKKKLILTDQLKQQTETKITELETKVS
                     NFKIEKFGEGGEYFLKQTEFMKPVQDRIFKAIETVAKDGSYDYVFDRSSEILLLYVNE
                     NYDLTSKVINEIEGK"
     gene            complement(2527..3093)
                     /locus_tag="PLX80_06245"
     CDS             complement(2527..3093)
                     /locus_tag="PLX80_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQY20432.1"
                     /translation="MKTSNLKLLIVIFSAIGFFISGNVQSQVKIGYVDSEVILKQLPE
                     AQEVQKELEDLQKLYLDTIQAKETDLKSKAETFKTRYEDAQKSVESGTVTSETDLKAL
                     NTEISGLQNELQTLSESLDNYKQSIQNSLLQKQAELFKPVKEKITKTIEDVSKSMKIN
                     FVFDKADGTLLYGDKDFDITFKVLDKLK"
     gene            complement(3143..5503)
                     /gene="bamA"
                     /locus_tag="PLX80_06250"
     CDS             complement(3143..5503)
                     /gene="bamA"
                     /locus_tag="PLX80_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQY20433.1"
                     /translation="MSPKLFIIIFLTFISLTSNLFSQETGPQKYRIASIDTKGNKNYE
                     KGTIISYSGLRQGAEISLPSDETRDAISRLWNIGIFSDVKIFVEKKFGDEVYLVIEVE
                     ELPRVESLEITGNDELSEDELKEKINIVPGEVISEQKLKDIEYNLEKAYAEEGFGMAE
                     VSVDKLLSANNEARIRIKIKEGNELTVNRIIVKGNKNVSKEDLIDAFENTSEGTWWKF
                     WEGADFDKAKYEEDKKLLIEFYRELGYKDASIVSEELDILPSKTDVNIIITVFEGSRY
                     FINDINITGNTVYGEDELLSRLKLKKGSVYNTKILQQNIYGNEEESDISSLYLDNGYL
                     SFNALINEEPAEKNDEVNLKIQISEGNPFRFGMVGFEGNDKTKDKVMRRELYTLPGNF
                     FTKSDIKRSMQQLAALNYFNPEKLSQDISLANDSTVQINYIVEEKSSDQFNASIGYSG
                     SFGITGALGLTFNNFDITKPLSGGAGQLMSFNWSFGTGGTYRTFDIGFQEPWLFDTPT
                     LFGFNIFDTRSNYSNLDQKETGASVNIGRRFKFPDDYFRGDWTLKYQNTNVINGDGVY
                     EEGIRNQFSIRQIISRSSVFEPVFPTTGTRVSNSTELSGGPFLPGTVEFVKNIFLTEA
                     FTPLTVNRKLVLFSTFQFSFINSFSKDKYLPPNETFYMGGNGLTYNTIALRGYDDRTV
                     GPQNQFGDPIGGRVTTKYGLEFRYSISQDPLPIFALIFAEAGNVWADINKTDPFDLRR
                     SVGFGTRLILPAVGLVGFDFGYGFDREAVDGIPPKWLFHFQFGRGF"
     gene            complement(5513..6295)
                     /locus_tag="PLX80_06255"
     CDS             complement(5513..6295)
                     /locus_tag="PLX80_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQY20434.1"
                     /translation="MTEPKAKDKIKQDELKAHGVIPAHIAIIMDGNGRWAKSKGLSRM
                     FGHNEGVNSARDIVEVSGQIGIKYLTLYTFSTENWKRPKTEVTMLMKLLIRSIRDETD
                     RLNQNNVKLIATGDLDNLPDKVRKELDDSMYTTKDNDLMTLNLALSYSGRWDILNAAK
                     KIAEEHKKNSFDLDSITEETFSEYLATKSLPDPDLLIRTGGEFRISNFLLWQLAYSEI
                     YIDNCYWPDFKRDNLYKAIYDFQKRERRFGMTSEQLIKNQKN"
     gene            complement(6353..7351)
                     /gene="trpS"
                     /locus_tag="PLX80_06260"
     CDS             complement(6353..7351)
                     /gene="trpS"
                     /locus_tag="PLX80_06260"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQY20435.1"
                     /translation="MTAVKKRIVSGIKPSGDLTIGNYLGAMKGWAADQHNDGYENFFF
                     VANLHAITVKQDPDLLKKRTLDIVAWLLTVGVDPLRSVIFVQSMIPAHSEICWILNNY
                     VTMGELGKMTQYKDKSQKSGKDGQLTGLFDYPVLMAGDVLLYDADEIPVGIDQVQHVE
                     LCRTIANRFNNQHGDTFKVPKATLSITSAKIMSLNNASEKMSKSESQDSYVLLEDSAD
                     KVIKKFKKAVTDSENKVAYGNGKPELSNLLTIFSEFSGKPVSDIENEYGDQGYGKFKT
                     DLGELVADKLTLLQKIYNEIRIDEERVMNVINDGNNKAKAIADSKMSEVKEKIGLL"
     gene            complement(7487..8500)
                     /locus_tag="PLX80_06265"
     CDS             complement(7487..8500)
                     /locus_tag="PLX80_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HQY20436.1"
                     /translation="MDNVTSTLESLLKIHSPTGFTNDVIKYISSLFIDNKDIKKTLTN
                     KGSLLISFDDDPELLLTAHIDTLGAMVKEVKGNGTLELTQLGSYPWNSFEGEYVTIRN
                     SKGKLYRGTFLMNNPADHVNKNLRTEKREYSNMSVRIDEPVQSESDVKKLDIETGNFV
                     FYDNRFEYTKSGFVKSRFLDDKACAAVLIELIKDLSKVKNKKKVGFYFSNYEEVGHGS
                     AIGIPDSVKELLVLDMAVVGDGCSGSEDSVSICVKDSSGPYDFDVSQKLKDLAVKNKI
                     KFNIDIYPYYGSDGSAALLAGYDVRVGLIGPGVSASHGVERTHRKGIAETYKLAKAYI
                     NNF"
     gene            complement(8500..8832)
                     /locus_tag="PLX80_06270"
     CDS             complement(8500..8832)
                     /locus_tag="PLX80_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQY20437.1"
                     /translation="MNSEKDGIFTSIVRFIKYNVNLVLIIVFFIGLISMTIFGNKGVL
                     QKMKLEEETREIENLIKLELVKTENLQKEIDALSSSDKRLEEVAREKFGMTKDGEKIY
                     KVIIDSTE"
     gene            complement(8873..9940)
                     /locus_tag="PLX80_06275"
     CDS             complement(8873..9940)
                     /locus_tag="PLX80_06275"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQY20438.1"
                     /translation="MNKIFALFFSVLNIVLITGGPSSEREVSVSSSKSILKALKESGH
                     NVTLLDPVNGAVLVSEEEIFKNAVKKESPGFKELDELRAASNRRILDCINSDLFDNTD
                     IVVLGVHGKFGEDGKLQNLLELRGVKYTGSGVFASAIAMDKDISKVIMKQAGILTPDW
                     FAVTGNNNSDYEELNKKINSTVGYPCVIKPNDEGSTVGLSIVQPDVEDIQLKDSIEMA
                     FHYSDKVIIEKYIKGRELTVPVIGDEAFPVIEIRPRDGFYDYEHKYTKGMTEYFCPAD
                     IPADIEKLAKENALKAHKALGCKVYSRVDFMLDDKNDLYCLEVNTLPGMTETSLVPKS
                     ALVNGMNFNQLIEKIIQLSLN"
     gene            10703..11890
                     /locus_tag="PLX80_06280"
     CDS             10703..11890
                     /locus_tag="PLX80_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY20439.1"
                     /translation="MPSYNVGSKDEVKGQKGIAHLFEHMMFQGSENVSRTGHFDMVMN
                     AGGVCNAFTGFDSTVYFNLLPSHNLELALWLESDRMNSLNVSESNLNNQISVVLEEKK
                     QVVDNVPYGTMIENYFKNVLYGSEYEVSVIGLEKDISSISVKDASNFHKNFYSPDNCI
                     LMISGSIDTDKTKDLVFKYFGDIHKKNLIKRNKNVIKVLTKNEKQTVPDNVQLPVVSV
                     CYQIPKSGSRAEYDLEYFTEILANNESSRLYRKYVYQKNLFKSVHANKLILKDAGILI
                     LTAMLNPSTDPALAAEIIKDEINLFYKSEISDKEFGKIRNQIEFRNVVKNLKMTNISI
                     ETVKNYHFFKDTNLINTSFEKYNSVTKEDVKETVKNLLIDKKNFTLTYLPAEKSLRKK
                     NKK"
     gene            11894..13183
                     /locus_tag="PLX80_06285"
     CDS             11894..13183
                     /locus_tag="PLX80_06285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY20440.1"
                     /translation="MRKFSAAEKIKTDVPSIDYREYTLDNGLRIILSRSKDIPMTAVN
                     TTFHVGSKDEDQNMTGMAHLLEHLMFEDSPNVPHGKFDEILNRNGGDSNAYTSWDCTG
                     YYIVLPSNKLETALWLESDRLAGFSISEESLRIQKDVVYEEKLLYVDNSPYGTVEEES
                     SKRLFVNSGYKSPVIGNMDQLMKVELDDVRKFYEKYYSPENAVLSVVGDINYDKTFKM
                     IEKYYGGIVSGGTPDRRDHSDEYLSSEIRVDIKDNVHLEGKFIFYRLPEMGTKDYYAM
                     SVLNGILSDGDSSRLYKELEYNNELVNEFDSNLYGMESTSIFAISALMLKGKSPDLAE
                     EKIDNVLEDIKNGNISDREIQKIKNRIETYFSAKKQTVVSLSEKFSFLKTFYNDINKI
                     NFEVLDYLSITKDDIVNTAVKYLDRNKRLVLNYKPVK"
     gene            13324..14706
                     /locus_tag="PLX80_06290"
     CDS             13324..14706
                     /locus_tag="PLX80_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY20441.1"
                     /translation="MNIEKINTGILDRSKPPKSGRPKDVNFPGFFETTLDNGISLVVI
                     EDRKLPLVTSRFIFKTGSYADHLWGKKYEGLSSITSELLTKGTGKMTASEIADETDYF
                     GASLSSGCDYDATYISTYSLKKYFDKVFEIAADVILNPSFPESELKRIKEQRINSLLS
                     MSDDGEYLADKIFRAEVYKNSPYALQSEGSKESVKIITTDLTKDFYKKYFRPDNLIIA
                     MVGDISPDEAMLKISSELKFDVKGSTKNPKIINPKINKGSKVFLYDKKGAVQSSIKMG
                     HLGIQRSNPDFITLNVMNTLLGGYFTSRINKNLREEKGYTYGSRSSFGCQKYSGDFSV
                     STEVKNEVTADTINEVLKEIKILKKEPVGKTELKNVKNYITGIFPLQLETPNAIAQKV
                     LSLKLNDMPSDYYNKFIKEINAVTSEQIMEAANKYLFEEDMIISVSGNVNDIRDSMKQ
                     FGKTVVIEEQ"
     gene            14731..15723
                     /locus_tag="PLX80_06295"
     CDS             14731..15723
                     /locus_tag="PLX80_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY20442.1"
                     /translation="MTDIKTQNVSDAESVKRLNQKYSELKNEISKVIVGQEYIIEQII
                     ISVLAKGHCLLVGVPGLAKTLLVKTLSEALDLKFSRVQFTPDLMPSDITGTEILEDDL
                     SGKKAFKFIKGPVFANIVLADEINRTPPKTQSALLEAMQEHRVTAAGNSYVLDEPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMFNLWLEYTSFEDELEIVRRTTSMYRPKVGIVFSK
                     DDIIEFQDLVRKVPVADEVIKYAVTLVAKTRPGNPDAPKIVNDYISWGAGPRASQYLI
                     LGAKTNCILNGRYSPDIDDVKKVALPVLRHRLITNFNAEADGLSIIKIIEELLK"
     gene            complement(15821..15964)
                     /locus_tag="PLX80_06300"
     CDS             complement(15821..15964)
                     /locus_tag="PLX80_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20443.1"
                     /translation="MPRVNPPRWLGGQGGVALKMQELFRKKIKIENYFEDNYSVIKKR
                     FRN"
     gene            16154..>16385
                     /locus_tag="PLX80_06305"
     CDS             16154..>16385
                     /locus_tag="PLX80_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20444.1"
                     /translation="MKRNLLITIFICFIFNVSPLRSQTPQYTLEVKNLTWSPGVNQFG
                     GSTLQFDLVFTHTDATVMQLAGWQFFFKLPQTA"
BASE COUNT         5133 a         3046 c         2729 g         5477 t
ORIGIN      
        1 tgtaaattca acattggatt tatgtccggg tctggcggca agtatctgac ctttgacagg
       61 cgcaccgata agcgcaagat cgccgataag gtcgagaagc ttatgtctgg caggttcgtt
      121 gcgaaatctc agctcacggt tatttaaaat tccattggaa ccgagtatta tactttcttc
      181 aattccaatt ttctttttca ttctctccag ttcttcatca gaggcatctt tatctacaat
      241 gacaattgca ttattgagat caccgccttt tattaaattc tgttcctgga gatattctat
      301 ttcacttaag aaacaaaaag ttctcgatgg tgcaaattct ttttcaaatt ctttatgcag
      361 attaaaaagt cctgtatgct gaacaccgag cgcgggatta ttataatcta tcattacgga
      421 aattctgaaa tcatctttga gaggaagagc tacaatatcg acattcgttt tatcatcatg
      481 ataatgaaca gtatcttcga tcacaagata atcacgcttt gcttcctgct ctacaatgtc
      541 agcttttttc aaagtctcta cataatcttt cgcactgccg tccatgatag gcggttcatt
      601 tgagtcaagc tcaataataa tattatcgat ctcacagccc ataatacttg caagcacatg
      661 ctctacagtg tgaacttctg caccatcctt tgcaattgta gtacctctcg aaatatcaat
      721 aacgtgatca atgtcagcag gtatttcagg agagtcaggg agatcagttc ttttaaactt
      781 aactccataa ttttcaggag caggtttaaa tgtcatgttt gatttatttc cggtatgaag
      841 tccaacgccg gataaggtga tctcattttt gattgtgcgc tgtttttcta acatataaag
      901 aattaaattt attttacttg ccgggggatt ctaacttttt tattctttct tcaagcttgt
      961 caagatttcg tattcttgct tctattttca gatcttctct gtaaggtctg aatctgtaac
     1021 cggtataaat acccggtaca gtaatagatt ttgaaacacc aacagatgag ccgataatta
     1081 catcatcaca tatttctata tgtccgacaa ttcctgcctg tcctcctatc atacatctct
     1141 tacctatctt agcagaacct gcaatgccga cctgtgcagc gatgacagta ttttcaccga
     1201 tggttacatt atgagcgatc tgaatctgat tatctatttt aactcctttg caaattcttg
     1261 tctcgccgat agtcgctctg tctattgaag tattagaacc gatctctaca ttgtcttcta
     1321 ttacaacatt accggtctgc ggtatttttt tataagtgta atcttcctgc ctggcaaatc
     1381 caaatccatc agatccaatg acagtaccgg aatggatgat cacattatct ccgatcactg
     1441 tatctttata aattgttaca tttggataga taagacaatt ctttcctaag attgaatttt
     1501 ttccaatata agtatttgca taaattcttg taccgtctcc gatcttacag tcattttcta
     1561 ttaccacatt aattcctgca taaacttttt caccggtctt tacattagtc ccaatactgc
     1621 acctttccga aataccaata tcagtaatgt cttcctttat ctcaaatttc tctaacagtt
     1681 ttaaaaaaga attgtacggg tcttttacac gtatcagtga aatatttttt cctgcattat
     1741 tatctgtaac ttcaaacccg tcagaaacta taacacatcc ggctttagtt gagtcaaaat
     1801 atttttcata tttcggattt gaaatgaaag tgatctcatg atcttcagca gtctcaattt
     1861 ttccaatacc tgtaacttca gcttcagggt cgccttcaag ttttccatta ataatatttg
     1921 cggcctcgct cagcttcatt tattttcctt caatttcatt aataacttta ctggtcagat
     1981 cgtaattttc attcacataa agaagtaaaa tttcactgct tctgtcaaac acatagtcat
     2041 agcttccatc ttttgcaact gtttcaatcg ctttgaatat tctgtcctgt acaggtttca
     2101 tgaattccgt ctgcttcaga aaatattcgc cgccttcacc aaatttttct atcttaaaat
     2161 tgctgacctt tgtctcaagt tcagtgatct ttgtttcagt ctgctgtttc agctgatctg
     2221 taagaatgag tttctttttt tcataatcat ccttaattat tttcagactg tcctggagct
     2281 gtgtgagttc agtctgccat tgagtgacaa gattatccag acgctgctgg gcatcacgtg
     2341 aatcctgcat tgattccaca attttttttg aatcaatata acctactttt gtctgagctg
     2401 aaagtccggc agacattaag aagactactg cagtaaaagc taatgtaata tttctgatga
     2461 ttttcattat cgctttttta taaaaagaat gttctgaata tttatcatcc caatatggaa
     2521 tggattttat ttcagcttat caagtacttt aaatgtaatg tcaaaatctt tatcaccata
     2581 aagcaaagta ccgtcagctt tgtcgaaaac gaaattgatc ttcatggatt ttgaaacatc
     2641 ctcaattgtc tttgtgatct tttccttcac aggtttgaat aattcagcct gtttctgaag
     2701 taacgaattt tggattgact gtttgtaatt atccagggat tcactaagtg tctgaagttc
     2761 attctgcaat ccggagatct cggtatttaa agctttcaga tcagtttctg atgttacagt
     2821 tccggactcg acactttttt gagcatcttc gtatctggtt ttaaaagtct cagcttttga
     2881 ttttaaatca gtctctttag cctgtattgt atcaaggtat aatttctgca agtcctcaag
     2941 ctctttctga acttcctgag cttcaggtaa ttgttttaaa attacttcac tgtctacata
     3001 tcctattttg acctgtgact gcacgtttcc tgaaatgaaa aatcctattg cagaaaaaat
     3061 tactattaat aatttcaagt tacttgtttt caaagaatat ctccttcttg tttttattta
     3121 agttaaattt atgtttttaa aattaaaagc ctcttccaaa ctgaaaatga aacagccatt
     3181 tcggaggaat accgtccact gcttctctgt caaatccata tccgaaatca aatcctacaa
     3241 gtcctactgc aggaagtatc agtctggttc caaaaccaac tgatcttctg agatcaaacg
     3301 gatcagtttt attaatgtca gcccacacat ttcctgcttc tgcaaaaata agtgcgaaga
     3361 tcggaagcgg atcctgagaa attgaatatc ggaattccag tccatacttg gtagttactc
     3421 ttccaccgat tggatcacca aactgattct gcggtccgac agtcctgtcg tcatatccac
     3481 gcaatgctat tgtgttataa gttaatccat ttccacccat gtaaaaagtt tcgtttggcg
     3541 gtaagtattt atcttttgaa aatgaattaa taaatgaaaa ctggaaagta ctgaataaaa
     3601 ctaatttcct gtttacagtt aaaggtgtga atgcttcagt cagaaaaata ttttttacaa
     3661 actcaactgt tccgggcaga aatggtccgc ccgataattc tgttgaattt gaaactcttg
     3721 tacctgttgt aggaaatacc ggttcaaaaa ccgatgatct cgaaatgatc tgccttatac
     3781 taaactgatt tcttatccct tcttcatata ctccgtctcc gttgataaca tttgtgttct
     3841 gatattttaa tgtccagtca cctctgaaat aatcatccgg aaatttaaat cttcttccga
     3901 tatttacaga tgcaccggtc tctttctgat cgaggttaga ataatttgac cttgtatcaa
     3961 aaatattaaa accaaacaaa gtcggtgtat caaaaagcca cggctcctga aaaccaatat
     4021 caaaagttct gtatgttcca cccgttccaa aagaccagtt gaaggacatg agctgtcccg
     4081 ctccgccgga aagtggtttt gtaatatcaa aattgttgaa tgtaagtcca agcgctccgg
     4141 tgataccgaa acttccgctg tacccgatcg atgcattgaa ctggtcagat gatttttctt
     4201 ctactatata attaatctgc acagtgctgt cgttagcaag tgagatgtcc tgacttaatt
     4261 tttcgggatt aaaataattt aaagccgcaa gctgctgcat acttcttttt atatcactct
     4321 ttgtaaaaaa attacccggt aaagtataaa gctctctgcg cattactttg tcttttgtct
     4381 tgtcatttcc ttcaaatcct accattccaa atctgaatgg atttccctca gatatctgga
     4441 tcttcagatt tacttcatca tttttttcag caggttcttc atttatcaga gcattaaatg
     4501 acagatagcc gttgtcaaga tataatgaac taatatcgga ttcttcttca ttaccataaa
     4561 tattctgttg aagtattttt gtattataaa cacttccttt tttaagctta agccgactta
     4621 ataattcatc ttcaccataa actgtattac ctgttatatt aatgtcattt ataaagtatc
     4681 tggaaccctc aaagacggta attataatat tcacatctgt tttggatgga agaatatcca
     4741 gttcttccga aacaatgctg gcatctttat aacccaactc tctgtaaaat tctataagta
     4801 attttttatc ctcttcatat tttgctttgt caaaatccgc tccttcccag aatttccacc
     4861 aggtaccttc actcgtattt tcaaaagcat ctataagatc ctctttggaa acattcttgt
     4921 ttccttttac aattattctg tttacggtca gctcattacc ttctttaatt ttgatcctga
     4981 tccttgcttc gttatttgct gacagcagtt tgtcaactga tacttcagcc attccaaaac
     5041 cttcttcagc gtatgctttt tccagattat attcaatgtc tttaagcttc tgctcactga
     5101 tcacttcacc cggaacaatg tttatttttt cttttaactc atcttcggaa agctcatcat
     5161 ttcctgttat ctcaagactt tcaaccctgg gaagctcttc tacttcaatt accagataaa
     5221 cttcgtcccc gaattttttt tcaacaaata tcttcacatc tgagaatatt cctatattcc
     5281 ataatctgct gattgcatct cttgtctcat ccgagggcag agagatctca gctccctgtc
     5341 tgagtcctga atatgatatg atcgttccct tttcataatt tttatttcct tttgtatcaa
     5401 tactcgcgat cctgtatttt tgaggtccgg tttcctggga aaataaattt gatgtgaggg
     5461 aaatgaatgt taaaaatatt attataaata atttgggtga cattttttga gtttaattct
     5521 tttggttttt aattaactgt tcgctggtca ttccgaatct tctttctctt ttctgaaaat
     5581 catatatcgc tttatataaa ttatctcttt taaaatcagg ccagtaacag ttatcaatat
     5641 atatttcgct gtacgccagc tgccataaaa gaaagttgct gatcctgaat tcaccgccgg
     5701 tcctgattaa aagatcagga tcaggtaggc tttttgttgc aagatattca gagaaagttt
     5761 cttctgtgat tgaatctaaa tcaaaactat tctttttatg ttcctctgct attttttttg
     5821 ctgcattgag aatatcccat ctcccgctgt aactgagtgc aagattcaaa gtcattagat
     5881 cattatcttt tgttgtgtac attgaatcat ccaattcttt tcttactttg tcaggaagat
     5941 tatccagatc ccccgtagca ataagtttta cattattttg atttaatctg tctgtctcat
     6001 cacgaataga ccttataagt aatttcatca gcatagttac ttctgtcttg ggtcttttcc
     6061 agttctctgt tgaaaaagta taaagcgtca gatattttat tccgatctgt cctgatactt
     6121 ccactatatc tcttgctgaa tttacacctt cattatgacc aaacattctc gaaagacctt
     6181 tggactttgc ccaccttccg ttaccatcca ttattattgc tatatgagca ggaatgacac
     6241 cgtgtgcttt taattcatcc tgttttattt tgtcttttgc ttttggctct gtcaaatgag
     6301 atgtttctac ttgtttaaaa ttatttttgt tcctttgtaa aaaaatgaat atttacaaaa
     6361 gtccgatctt ttcttttact tcagacattt tactatcagc aatagcttta gctttgttgt
     6421 ttccatcatt gatcacattc ataactctct cttcatcaat tctgatctca ttgtaaattt
     6481 tttgaagtaa ggtcagctta tcagctacga gttcaccgag atccgtcttg aatttcccat
     6541 atccctgatc accatattca ttctcaatat cacttacagg ctttcctgaa aattcagaaa
     6601 atatagttaa aagatttgaa agttcaggtt taccgtttcc ataagcaact ttattttctg
     6661 aatctgtaac ggcttttttg aatttcttaa ttaccttatc agcactgtcc tcaagtaaaa
     6721 cataactgtc ctgtgattcg ctcttgctca tcttttcgga tgcattatta agtgacatga
     6781 tctttgcaga tgttatagac aaagtagctt tcggaacttt aaaagtatct ccgtgctgat
     6841 tattaaatct atttgcaata gtcctgcata gctctacatg ctgtacctga tctatgccga
     6901 cagggatttc atctgcatca tataaaagta catcacctgc catcagcacc ggataatcaa
     6961 acaatcctgt cagttgtcca tctttgccgg acttctggga tttgtcctta tattgtgtca
     7021 tctttccaag ctcgcccata gtcacataat tattcagtat ccagcaaatc tcactgtgtg
     7081 ccggtatcat agactgaacg aatatcacag atctgagcgg atcgactccg actgtaagaa
     7141 gccaggccac tatatcaaga gttctttttt ttaataaatc aggatcctgt tttacagtaa
     7201 ttgcatgaag attagctacg aagaaaaaat tttcataacc atcattatgc tgatcagctg
     7261 cccagccttt catcgcgcca agataatttc caatagtaag atcacctgac ggctttatac
     7321 ccgagactat tctttttttt actgcagtca aagcttctat ttattattaa aaattttcaa
     7381 aaaatataaa aacataacag attaaaatat actcttctta attatttaaa atggaatcag
     7441 ttttacaatt atgtaaaata tggtatttaa ttatcacact aaaatttcaa aaattattta
     7501 tgtaagcttt ggcaagttta tatgtttcgg caattccttt ccggtgagtt ctttcgactc
     7561 cgtgtgaagc ggaaactccg ggtcctataa gaccgactct tacatcatac ccggcaagca
     7621 gcgcagcgct tccgtcagaa ccgtaataag ggtatatatc tatattgaat ttgatcttat
     7681 tctttacagc aagatctttc agcttttgcg aaacatcaaa atcataagga cctgaagaat
     7741 ctttaacgca aattgaaact gaatcttcag aacctgaaca gccgtcacct actactgcca
     7801 tatccagcac cagaagctct ttcacagaat caggaattcc aattgctgat ccgtgaccga
     7861 cctcttcata atttgaaaaa taaaatccaa ccttcttttt atttttaact tttgacaaat
     7921 cttttatcaa ctctattaat accgcagcgc atgccttatc gtcaagaaat ctggatttca
     7981 caaatcctga tttagtatat tcaaatctgt tatcatagaa tacaaaatta cctgtttcaa
     8041 tatccagctt ttttacatcg gattcacttt gaaccggttc atcaattctt acagacatat
     8101 tggagtactc acgcttttca gttctgagat ttttgtttac atgatctgca ggattattca
     8161 taagaaaagt cccgcggtaa agtttgcctt tagaatttcg gatagtgacg tattcacctt
     8221 caaaagaatt ccatggatag gatccaagct gagtaagctc cagagtcccg ttgcctttta
     8281 cttcttttac catagcaccg agcgtatcaa tgtgcgctgt caaaagcagt tcagggtcat
     8341 catcaaaaga tatcagaaga gaacctttgt ttgtcagagt cttttttata tctttattat
     8401 caataaataa agatgaaata tattttatca catcatttgt aaatccggtg ggcgagtgga
     8461 tctttagaag tgattcaagt gtcgaagtta cgttatccat tattctgtgc tgtctattat
     8521 tactttataa attttttctc cgtcttttgt catgccaaat ttttctctgg cgacctcttc
     8581 aagtcttttg tcagatgaac ttaaagcgtc tatttctttc tgaagatttt ccgttttaac
     8641 aagttcaagt tttatcagat tctcaatttc cctggtctct tcttcaagtt tcatcttttg
     8701 caatactcct ttgtttccaa aaattgtcat ggaaataagt ccaataaaaa atacgataat
     8761 aagtacaagg tttacattgt attttataaa ccggactata cttgtaaata ttccgtcttt
     8821 ttcgctattc ataaaaatta tatatcaaaa attatttttt aacaaatcca tttcagttta
     8881 atgaaagttg aattattttt tcaatcagct gattaaaatt cattccgttc accagtgccg
     8941 attttggaac gagtgaagtc tccgtcattc ccggcagcgt gttcacctca agacaataaa
     9001 gatcattttt atcatcaagc ataaaatcaa ctcttgaata aactttacat ccaagtgctt
     9061 tatgagcctt caaagcattt tcctttgcca gcttttcaat atctgcaggt atatctgcag
     9121 gacaaaaata ttcagtcatg ccctttgtat atttatgctc ataatcataa aatccgtctc
     9181 tgggtctgat ctcgatgaca ggaaaagctt catctccgat cacaggaaca gtaagttctc
     9241 ttccttttat atacttttca ataattactt tatcagaata atgaaaagcc atttcgatag
     9301 aatcttttaa ctgtatatcc tccacatccg gctgcacaat gctgagtccg actgtacttc
     9361 cctcgtcatt tggctttata acgcagggat atccgacagt ggaattaatt tttttattaa
     9421 gctcctcata gtcagaatta ttgttaccgg taacagcaaa ccagtccgga gtaagaatac
     9481 ctgcctgttt cattattact ttggaaatgt ctttatccat tgcaatagca gaagcaaaca
     9541 ctcccgaacc tgtatatttt actccgcgga gttcgagcag attctgcagt ttgccgtctt
     9601 cgccgaattt cccatgaacg ccgagtacta ctatatctgt gttatcaaac agatcagaat
     9661 ttatgcagtc aagtattctt ctattcgaag ccgcccgtaa ttcatcaagt tctttaaatc
     9721 cgggtgattc ttttttaacg gcatttttga aaatttcttc ttcgcttaca agcactgctc
     9781 cgtttacagg gtcaagtaaa gtcacattat gacctgattc ttttaaagct tttaatatac
     9841 ttttagaaga tgaaacagaa acttctcttt ctgacgaagg tccgcctgtg atcaatacta
     9901 tattcaatac actaaaaaat agagcaaaaa ttttgttcaa tatacttatt tcaaattagt
     9961 ataaaaatgt taaattcatg ataatattca ggatgaattc aggtaacctc taaaacattt
    10021 tatctcaaaa ttgaagatcc attattttct gctaactttt ttgaataacc aaaaaaagta
    10081 acaaaaaaag tagtcccggg tacccgggat gataaactgc ctgatatttg tttgaaacgg
    10141 agagtctttg ctccaattgt aatattttca gtcaaccaac cggggcaatg ccattaagta
    10201 aagggatttt tatttaataa ttagattaat atgaatttat tattcaaaaa tccaagctcc
    10261 tcaccttttt ttcaaagcga ggccgggagg ggtttgctga cagcggcaat acttcgcctt
    10321 ttatataatg gctttggctc tccggatgtt aagagttttt caaatgcaaa atgatttgga
    10381 aagatgtaat tgatattcga aattcaattt tccactgctt ttaattttca aataagttag
    10441 ttttgtaagt ttaaaatctg caaatagatc tgatcttaat tttatttcgt atataaaatt
    10501 agtacagatc agatagatct ggaaatttcc ggtcatattt taattatctt ataattaatt
    10561 caatattaaa acttctaaag taaattacaa tattttatca ggcacagaaa attctttaaa
    10621 tcttgagttt gaagaatttg atctgccaaa cggactacac tgtatccttt ataaggatat
    10681 ctccaagcca aatgtctgtg taatgccttc ctataatgtc ggctcaaagg acgaagtaaa
    10741 aggtcagaaa ggaatagcac atttgtttga acacatgatg tttcagggat ccgaaaatgt
    10801 cagcaggacc ggtcattttg atatggtaat gaatgccggc ggagtttgta atgcgtttac
    10861 aggatttgac tcgactgttt attttaatct gctcccttct cataatcttg agctggctct
    10921 atggcttgag tctgacagga tgaactcatt aaacgtttca gagagtaatc tgaataatca
    10981 gatcagtgtc gtacttgaag aaaaaaaaca ggttgtcgat aatgtgcctt atggtaccat
    11041 gatcgagaat tattttaaga atgtacttta tggatcagaa tatgaagtgt cagtgatcgg
    11101 acttgaaaaa gatatcagtt cgatttcagt taaagatgca tcgaatttcc ataagaattt
    11161 ttattcgccg gataattgta tattaatgat atccggttcg atagatacag ataagacaaa
    11221 ggatcttgtt tttaaatact ttggagacat tcataaaaaa aatcttataa aaagaaataa
    11281 gaatgtcata aaggtactga ctaaaaatga aaagcagaca gttccggata atgttcagct
    11341 tcccgttgta agtgtctgtt atcagatccc gaaatccggc agcagagcag aatatgatct
    11401 ggaatatttc acagagatac ttgccaataa tgagagcagc cgtctttaca gaaaatatgt
    11461 ctatcagaaa aatttattta agtcagttca tgccaataaa ttaattctaa aagacgcggg
    11521 gatcctgatt ttaactgcaa tgctgaatcc gtcaaccgat cctgcgcttg cagcagagat
    11581 cattaaagat gaaattaatt tattttataa aagtgaaata agtgataagg aatttggaaa
    11641 gatcagaaat cagatcgaat tcagaaatgt tgtcaaaaat cttaagatga caaatatttc
    11701 aatcgagaca gtaaagaatt atcatttttt taaagatacg aatcttatta atacttcttt
    11761 tgaaaagtat aattctgtca ctaaggaaga tgtaaaggaa actgtaaaaa atttattgat
    11821 cgataagaaa aattttacac tgacttatct gcctgcagaa aagtctttaa gaaagaaaaa
    11881 taaaaaataa tacttgagaa agtttagtgc cgcagaaaaa ataaaaaccg atgtcccttc
    11941 aatagactac agggaatata cgcttgataa cggattacgg attatactca gccgctcaaa
    12001 ggatattcct atgacggcag tgaatactac attccatgtc ggatcaaagg atgaagatca
    12061 gaatatgaca gggatggcgc atttactgga acatctgatg ttcgaagatt ctccgaatgt
    12121 accacatgga aaatttgacg agatcctgaa caggaacggc ggcgacagca atgcatatac
    12181 aagctgggac tgcacgggat attatattgt acttccatcc aataaattag aaacagcgct
    12241 ctggctggaa tcggacagac ttgcaggatt tagtatttca gaagaaagcc ttcgcataca
    12301 gaaggatgtc gtgtatgaag aaaagctttt gtatgttgat aattctcctt acggcactgt
    12361 cgaagaggaa agctctaaac gtcttttcgt aaacagcggc tataaaagtc ctgtgatcgg
    12421 aaatatggat cagcttatga aagtggagct ggatgacgtg agaaaatttt atgaaaaata
    12481 ttacagccct gaaaatgcag tcctgtcagt agtaggcgat ataaattatg ataagacatt
    12541 taagatgatc gaaaaatatt acggtggaat agtgtccgga ggaacacccg acagaagaga
    12601 tcattcggat gaatatcttt cttctgaaat aagagtcgat ataaaagaca atgttcatct
    12661 tgaaggaaaa tttatttttt acagactgcc tgaaatggga acgaaagatt attatgcaat
    12721 gagcgttcta aacgggatac tttctgacgg tgacagctca agactttaca aagaacttga
    12781 atataataat gaacttgtaa atgaatttga ttcgaatctt tatggaatgg aaagtaccag
    12841 tatctttgca ataagtgctt tgatgctgaa aggcaaaagt cctgatctgg ctgaagaaaa
    12901 aattgacaat gtattagaag atattaaaaa tggaaatatt tcagacaggg aaattcaaaa
    12961 aattaaaaac agaattgaga catatttcag cgctaagaag cagactgtag tttcattgtc
    13021 agaaaaattt tctttcctga aaacctttta taatgatatc aataaaataa attttgaagt
    13081 gctggattat ttgtcgataa caaaagacga tattgtaaat actgctgtaa aatatcttga
    13141 caggaataag agactggtac tgaattataa gccggttaag taaagacttt ttaattcatt
    13201 atccgcagag tccgcagaat aagtgactct gccggaacaa aattgggaat aaaaggagta
    13261 acataaaatc aatcttcttt gagccttcga gtctttgagg ttaaattaaa attttaaaat
    13321 aaattgaaca tagaaaaaat aaatacagga attcttgaca ggtcaaagcc acctaaatcc
    13381 ggcagaccca aagatgtaaa cttccccgga ttttttgaaa cgacacttga taacggaata
    13441 agtctggtgg ttattgaaga caggaagctt ccgttagtga cgtcaagatt catctttaag
    13501 acaggctcgt atgcagatca tttgtggggt aaaaaatatg aaggactttc ttccattact
    13561 tcagaattgc ttactaaagg aacaggtaaa atgactgctt ccgaaattgc tgatgaaacg
    13621 gattacttcg gagcatcact ttccagcgga tgtgactatg atgctactta catcagcact
    13681 tacagtctga aaaaatattt tgacaaagtt tttgaaattg cagcagatgt aatactgaat
    13741 ccttcatttc ccgaatctga attaaaaaga attaaggagc agagaattaa ttcgcttctt
    13801 tcaatgtcag atgatggaga atatcttgcg gataaaatat ttcgtgcaga ggtttataaa
    13861 aattcacctt atgcattaca gtctgaaggc agtaaggaat cagtaaaaat tattacaact
    13921 gatctaacaa aggattttta taaaaaatat ttccgtccgg ataaccttat cattgcaatg
    13981 gttggtgaca tatctcccga tgaagcaatg cttaagatca gcagtgaatt gaaattcgat
    14041 gtaaaaggca gtacaaagaa tccaaaaatt atcaatccga agatcaataa aggcagtaaa
    14101 gtatttctct atgataaaaa aggtgcggtt caatcttcaa taaaaatggg acatcttgga
    14161 attcaaagaa gcaaccctga ctttataacg ctgaatgtga tgaatactct gcttggcgga
    14221 tattttacat caaggataaa taaaaatctg agggaggaaa aaggttatac atacggatcg
    14281 aggtcttctt ttggttgtca gaaatacagc ggagattttt ctgtatcgac tgaagtgaaa
    14341 aatgaagtta cggctgatac tataaatgag gtgttaaagg aaattaaaat tctgaaaaaa
    14401 gaaccagtcg gtaaaactga acttaagaat gtaaaaaatt atatcacggg aatattcccg
    14461 ctgcagcttg aaactccgaa tgcaattgca cagaaagtac ttagtcttaa actcaatgac
    14521 atgccttctg attattacaa taaattcata aaggaaataa atgcagttac ttcagaacag
    14581 atcatggaag ctgcaaataa atatcttttt gaggaagata tgataatttc tgtttcagga
    14641 aatgtaaatg atatcaggga tagcatgaaa caattcggaa agacggttgt aatagaggag
    14701 caataaaaaa atatttaaat tttaaattga atgactgata taaaaactca aaacgtgtct
    14761 gatgcagaat cagtaaaaag actgaatcag aaatactctg aacttaaaaa tgaaatctcc
    14821 aaagtaatag taggtcagga atatataata gagcagataa ttatttctgt tctggctaag
    14881 ggacattgtc ttttagtcgg tgtgcccgga cttgcaaaga cactactggt aaagacttta
    14941 tccgaagcgc ttgatctgaa attcagccga gtacagttca cgcctgatct gatgccttct
    15001 gatattacag gtacggaaat actagaggat gatctttcag gaaagaaagc attcaaattt
    15061 atcaaaggac cggtgtttgc aaatatcgtt cttgctgatg aaataaacag aacacctccg
    15121 aagacacaat cagctttact cgaagcgatg caggagcaca gagtaactgc agctggaaat
    15181 tcttatgttt tagacgagcc gttcttcgta cttgccactc agaatcctat cgagcaggaa
    15241 ggtacgtatc cgctgcctga agcacagctt gacagattta tgttcaatct atggctggaa
    15301 tatacaagct ttgaagatga gctggaaatt gtaagaagga cgacgagtat gtacagacca
    15361 aaggttggaa ttgtattttc aaaagatgat ataatagaat ttcaggatct ggtgaggaaa
    15421 gttccagtag cagacgaagt aataaaatac gctgtaacac ttgtcgcaaa gacccgtcca
    15481 ggaaatcctg acgctccgaa aattgtaaat gactacataa gctggggtgc aggtccgaga
    15541 gcatcgcagt atctgatact tggcgcaaag acaaactgta tcctgaacgg gagatattct
    15601 ccggatatag atgatgtaaa aaaagtcgcg ctgcctgtcc tgagacacag actcattaca
    15661 aactttaatg cggaagcaga cggtctcagc ataataaaaa ttattgaaga gcttctgaag
    15721 tagtgaatgg atttttgaaa tccattcttt ctttatattc attagtttta ccgtgacaca
    15781 gattagtaat ctgtgttcaa ctccctttca aaacccacct ttaatttcta aatctttttt
    15841 ttataacgct gtaattgtct tcaaaataat tttcaatttt tattttcttt cggaaaagtt
    15901 cttgcatttt aagagcaaca ccaccctgcc ctcccagcca gcggggaggg tttacccgtg
    15961 gcatagatta gtaatctaaa aggtttgatg tattttttaa tttattcatc acaataaagt
    16021 tataatactt ttttggaact ccaaaaacgg cgggaaaata ataataaaag tacatgcact
    16081 tttaaaactt gaataaggta ttttaaaaag ctaatttgat gaagttttaa aatatcaatt
    16141 aattaagaat gctatgaaaa gaaatttact aattacaatt ttcatttgtt ttatttttaa
    16201 tgtaagtcct cttagatcac aaacacctca atacacatta gaagtaaaaa atttaacctg
    16261 gtcaccagga gttaaccaat ttggtggttc tacgcttcag tttgatctgg tattcacaca
    16321 tactgatgct acagtaatgc aattagcagg atggcagttc ttctttaaat tgccgcaaac
    16381 agcag
//
LOCUS       DAORVQ010000055        16311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1530_length_16311_cov_6.964690, whole genome shotgun sequence.
ACCESSION   DAORVQ010000055 DAORVQ010000000
VERSION     DAORVQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16311
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_16311_cov_6.964690"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PLX80_06310"
     CDS             <1..1348
                     /locus_tag="PLX80_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20445.1"
                     /translation="NCKNYRLSKMNLFLRLNICLIFIFLITFFQISETISQEYWSRID
                     SPVNVELRNCFFINAETGWIAGESGTVIRTDNGGNSWAVQNTNVSGAIEDIFFINSLT
                     GWAVSLEIFPDSNSFLGTIILSTTDGGNNWNRSMFPDTNIFMKTVYFQNANTGFLGGI
                     PSTIQRTTNGGLTWTPTQTDSNLIFLLPVFKIKFYDQNTGYACGGFRDIAGLVWVTTN
                     AGLVWRGTELAPEPFFDITMTSSNNSVISGGDLEFGSSVARTSNTGTNWYYDTLGVFG
                     LATGVSFRTPYECWMTGSYSEKFLLSKDSGITWTSLYTLDSTALFDVTFPDTTYGFAC
                     GVQGTIYKYDRTSTSIFNETNTSNDLSFNLYQNFPNPFNPITKISYELPVKSYQFVSL
                     KVFDVLGNEVAELVNEQQSPGSYSVDFDGSNFSSGIYFYELNIGEYSEVRKMVLLK"
     gene            complement(1645..4983)
                     /locus_tag="PLX80_06315"
     CDS             complement(1645..4983)
                     /locus_tag="PLX80_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQY20446.1"
                     /translation="MRPIRKLIFMGILVFSLFNLSISENGISTDTKSVFAQQDNSVSN
                     SVWTDKADIVINDITKVIPQKYRYLNLNLNSFRNIMTAAPFEFTDRAVASPLIIELPH
                     PDGGMSKFYVTEYSMMEQGLADQYPDMKTYNVKGIDDPYALGKIDFTAFGFHAMVLTP
                     NGDYFIDPLSMDNTENYISYFKSDYFSNQTFECLVTEVTENTELINSHNSDMTGQQLR
                     TYRLACAATGEYTAFHGGTVALGLAGIVTAINRVNQVYERDLSVRMTLVANNNLIVYT
                     NSGTDPYTNNNGVTMLGQNQTNCNAVIGSANYDIGHVFSTGGGGVAGLSVVCVNASKA
                     RGVTGLPSPIGDPFYIDYVAHEMGHQFGGNHTFNGSSGNCAGGNRNGSTAWEPGSGST
                     IQAYAGICSPQDLQSNSDDYFHTGSVSEMTAFTQTGNGNSCPVTTNTGNTPPTVTVPS
                     GGFTIPISTPFELTGSATDTETPGSLTYCWEEFDLGAAGAPNSPVGNAPIFRSFNPVV
                     SPTRTFPKLSNLLNNTSTIGEILPTYTRSLTFRLTARDNSAGGGGINFSTVAFSVNNS
                     SGPFLVTSPNTGVSWNAGAQTVTWNVANTTAAPVNCANVDILLSTDGGNTFPTTILSN
                     TPNDGSQSVTIPNTATATARIKVKANGNIFFDISNTNFTIVGGPSAPPCEDFTSGTFP
                     PPPLTIDFTGTNRWSRNTVSAYGIGTGSAKFDFYNAQFNENQSIVSDNFVATLTGDYL
                     TFDEAYAPYTDVNFGPDTLLVEASSNSGGSYTTLATLIGRQDGTGELNTAPPNVNAYT
                     PTNSEWASKIYALPAGTNKIRLKAKSGYGNNLYIDNLCVKTLPNATIAGIGNTPQGFY
                     RGTPGIYSGIPDSVNIYLHRTDFPNIKVDSAFITLSNSCETFPTFSKALTGNYYIVVK
                     HRNSIETWSRSGGEAYTRGGFFYYDFLEENYAYNNNQFQINSTNNLWGMYGGDCNQDG
                     VIEIADLSIIDNDAFNFAGGYIVSDVNGDNFADINDYAIADNNAFNIVTIQAPPGSAP
                     PPAEDKNINPVFKSDAERNKYEMSLRISKEINVKTEVKQVSTSELNEVLKQRSERDKL
                     RTTPLSKNSEVKINSVNRTQVRRK"
     gene            complement(5260..8346)
                     /locus_tag="PLX80_06320"
     CDS             complement(5260..8346)
                     /locus_tag="PLX80_06320"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQY20447.1"
                     /translation="MKKLTFLRTWITSAIVAVFILVAGTQVFQGNEKTDFQKHTEQFY
                     DYDETDPYIDRPTSESRMHMTENDDVLDVITDGTGFDNFEIGTDNAEQMLVSNPRNPM
                     NMQFGVNGGGGQNAYYTTNGHDWTSSNPAYHASTCCDPWSAFDSLGNLYYGSGVANQY
                     VYKSTNGGQTYGSPVLSVNGNDRNTLAADQTNGPYKNYLYAAITPGNFSRSINGNATW
                     TTTYSPSNTIPGVMIAVGPNGTTQGGCVMYVTNTGTSSNVTYTFHRSLDGGATFAVRS
                     SLTVAGYVGTLNTAGRLVINNGRTRPYPMIAMDNSYGPNRGRLYLVYASNVPAGNGNK
                     PDIILQYSDNQGLTWSSRITVNDNANPQLSDQWFPAIWCEKETGRLYIKWYDTRENPA
                     AYAVNVYGTYTDNGGTTFAPNQKLTTASWTYPSPACAPNTNCYRGDYDGMTANPKTSF
                     SVWYDGRAGTYKNVGAYFPDYAMTVSPTETTIYTTNDFRYIDVNVPAVKLYTDIVVFQ
                     TSITPTPGSGSLIADFPEGNTLSSPYPKTADMRIRTIGTVTPGVYTVTVTGSGTNGTP
                     VHKRTVSVTVNSTLSAVPCEDFSGAMFPPDDIDIDFAGTLRWSRNNVSAYGSGNGSAK
                     FDFYNAGVGPVQSLLSKSFAPVAAGSYLTFDEAYAAYSPAFGPDTLVVESSTNFGSSY
                     TVLATLTGNGDGTGELTTAPPTTSAFTPTSSQWASKIYALPVGTNKIRLRAKSGFGNN
                     LYVDNICVQSLPTAVINSVGLVNEAMFIPNTPFWRLQDTVRVYLHRTDFPNIAVDSAL
                     AVTNSSAVLNLLFEKALNGNYYKVVKHRNSLETWSNTGTGYSRGSNTHFNFVTPDGQA
                     YGNNQAIVSLLPFYRGVFSGDCNYDGIIDIADLAIIDNQVSNFTVGYVIGDLNGDLFV
                     DITDYAYADNNANNFITVSKPPGAEPLPPVEESAPVFENEEAKAKYELHLKSGKFNNN
                     SVTENKKNSTELNEVLKKRQSEIQNNKDKVKPKINNQTPPINSAPQYNSGKGPGEN"
     gene            complement(8681..11563)
                     /locus_tag="PLX80_06325"
     CDS             complement(8681..11563)
                     /locus_tag="PLX80_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20448.1"
                     /translation="MKKLFLTIPLIVLGFYFFTGQSQDSKPEKWSQDTRMTGALDPGN
                     RISAPLPMEYNDFVPNNQPMYNNTPNGVLVVNPNFRVLPRTSPFSYQTEVIITRHPGN
                     PLIMYGSSNAISNAGGPLFISEGAYVTTDGGTTWFGSDTMKNTVGTILSGHGGDPGIT
                     IDKDGRFIISHLGGGIKANFSTNNGLNWSNDVTITTGSQDKNLTTTDDSPTSPYYGRS
                     YTAWSRFNAASPPIAFSYTTNGGVSWSAATDINVPPAGHYSQGVDLRTGPNGEVYVCW
                     AQPTTSGSFTEDYAGFAKSTNGGVNWTIVNNNIFDMNGIRGNLLSTNIRVNSFPRIDV
                     DRTCGPRAGWIYIVVSQKNLAPAGTDADIVIHRSSDGGTTWSAGIKVNQDALNNGKSQ
                     WFPVVRVDESGGVNVVYYDNRGTTNTDTAEVFMSRSMDGGSTWSDLKVSDARFRPTSL
                     ALSGVAGGYQGDYIGLTSSLVEGNSVNGNQRIWPLWMDNRLGGFQYQAYTAKIELLPK
                     NPCTGCEDFTSSAFTPKYFHLNFSGTQYWTRSAASAYGVGSGSAKYDFFSDNVRTPQS
                     LITDFEAVPTGYYLTFDEAYAPYSAGFPGPDTLVVESSVNGGSSYTTLAVLQGLFPSG
                     GELNTAPATTNSFVPTASQWASKIYSLPAGTNRVRLRAESGFGNNLYLDNICVQSFPA
                     PSTTSNIGLVPEGFYRALPFPQTIPDTVRAYLHRTDFPNIIVDSATSYLNTNAVALSP
                     FTRALTGTYYLEIKHRNSLETWSKAGGVSYTRGSSINFNFINPVNQAYNNNQAFIDPA
                     PYYGLYGGDVNKDGVIDLSDLATIDNDAFNFAAGYVISDLNGDNFADLTDYALADNNA
                     FNIVTKQTPPGASPAPETVNNDNPVFTTEAEKTKYEMHLKIMREKGLSEEVNKNQKSE
                     YELNEVLRLRKERDQNRTAPVRKTNEVKSNVRENSVKSGSSFGQSK"
     gene            11829..13142
                     /locus_tag="PLX80_06330"
     CDS             11829..13142
                     /locus_tag="PLX80_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20449.1"
                     /translation="MPRKFILSSILIFCSVFLISELSVSQDYWIRQQTPTTKRLTNCF
                     FINNNTGWVTGDSGLVMKSTDSGVSWQLQNSNTFHDIFSVHFINDRLGWATAWVFNPL
                     PDEFPGTLLLKTINGGINWTSQMYADTNSYYGSLFYLDSLNGFLGGIPQFIVYTTDNG
                     VSWNDAVSDSIGFNGFPVTFITFLNADTGYASGGVQDIAGLVWKTVNNGRNWSPTAVG
                     PDPINDIYFFSADTSIGLSGDFKFGASFYRTNNDWLNSFNVNLGYIGMAQSIDFRIRN
                     DGWITIGYLSKLFRSTNAGDNWVKMDIPDSSAIFDIQFIDSAYGWCVGDHGAVYKYDI
                     MTSVNSNEISNEINSFNLFQNFPNPFNPVTKISYELQNTDNGIVTLKVFDVLGNEIAT
                     LVNEKQTPGRYNVTFDGTGLSSGVYFYRLSLNGNFISGKTMMMLK"
     gene            complement(13202..14590)
                     /locus_tag="PLX80_06335"
     CDS             complement(13202..14590)
                     /locus_tag="PLX80_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HQY20450.1"
                     /translation="MKKIGIIGGGFSGTMSAVQLIEKSNAPVEIILINESETLNKGIA
                     YRPYSGKHILNVITGKMSAYPDKPDHFLDWVTSRKDYADKDRNLIANSFLPREHYGEY
                     LLEIWKNAVESAESKHIKITVINKFVVDLDVTDNSVSITLDDNSILLLDYCVITTGNN
                     IPRNPAIKNKEFYQSKNYFRNPWKIDSVKELNNDLPVLIIGNGLTMVDTVLGLLEQGF
                     KGEIFSISPNGFNILPHRHNGLKYTKLTEELDENAGLYELLKLVNKHIKAVREYGVSA
                     EPVIDSLRPFTQKIWKSFSDADKKLFMSRLRHLWGVARHRIPLHTHDKIQKLRIDGKL
                     HINSGKIIDFTENENYITVEYFDKKENEVQSINVSRVINCTGPETDLMNLEKSFLKNC
                     LVKGIISQDNLKLGIKTETDTFQIINSEGNSHKNLFTIGSNLKGEIWETTAVNELRGQ
                     AEKLAEKLKVSQ"
     gene            15082..16041
                     /locus_tag="PLX80_06340"
     CDS             15082..16041
                     /locus_tag="PLX80_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20451.1"
                     /translation="MKKLILLFLFILISTYASSQTLEENLQQVGPNYGKLYLQPLVDA
                     MGTDVNSNFFYTAYVPYDSKKPVQFNIGLRFRAMNTFLSSVDQVFDYTYSDTLTVNGV
                     RYSGAYSVENAPTVIGNKTEAVAKFTSNGVYYPEFDLELIGGIINTTLVPLVIPEITF
                     GTVYGTDASIILLPTIDLNDLGKFRMFGFTLRHNFSHYVKDSPVDYSIMVGYQRMSLT
                     ENNNNDLWKSKSFFINGQLSKTFGGIFTPYMAIQYEQFKADISYIFNNFGQVFPVSFS
                     VDGATKFRGVLGATVKAGFFAFNVDANLGEKFALSCAFNFIIM"
BASE COUNT         4813 a         3050 c         2891 g         5557 t
ORIGIN      
        1 gaattgtaaa aattatagac tatccaaaat gaatttattt ctccggctaa atatctgtct
       61 gatctttata ttcttaataa ccttttttca aataagcgaa actatttccc aggaatactg
      121 gagccgtata gatagtccgg ttaacgttga actaagaaac tgttttttta taaatgctga
      181 aacaggatgg atagccggag aatccggaac agttatcaga actgacaacg gaggcaacag
      241 ctgggctgtc caaaacacaa atgtatccgg agctatagaa gatatatttt ttataaatag
      301 tcttaccggc tgggctgtgt cacttgaaat ttttcctgac agcaattcat tccttggcac
      361 aattatatta agtacaacgg acggtggaaa taactggaac agatcaatgt ttcctgatac
      421 aaatattttt atgaagacag tttattttca gaatgcaaac acaggatttc ttggtggtat
      481 cccttctacc attcaacgga ctacaaacgg aggactcaca tggactccaa cccagactga
      541 cagtaatctc atattccttc tcccggtatt taaaataaaa ttttatgacc agaatacagg
      601 gtatgcctgc ggtggattca gagatattgc agggttggta tgggtcacaa caaatgcagg
      661 tctggtctgg agaggtactg aattagcacc cgaacctttt tttgatatta caatgacttc
      721 atccaataac tccgtaatct caggaggtga tctggagttt ggttccagtg tggctaggac
      781 atcaaacact ggcactaact ggtactatga tactctcgga gtatttggtc ttgctacagg
      841 tgtaagcttc agaacacctt acgaatgctg gatgacagga agctattcag aaaaatttct
      901 cttatcaaaa gattcaggga ttacctggac ttcactttat actttagaca gtacagcttt
      961 atttgatgtt acttttcccg atactactta tggatttgcc tgcggagttc agggtacgat
     1021 ttataaatat gacagaactt caacttccat ttttaatgaa accaatactt caaatgatct
     1081 ttcatttaat ctttatcaga attttccaaa cccattcaac ccaattacaa aaataagtta
     1141 cgagttaccg gttaaaagtt accagtttgt aagtctgaaa gtatttgatg ttttgggaaa
     1201 tgaagttgct gagttagtga atgaacagca aagtccggga agctattcag ttgatttcga
     1261 tggaagtaat ttttcaagcg ggatttattt ttatgaactg aacattggtg aatattctga
     1321 agtaaggaaa atggttcttc ttaaataatt ttggttaact attagttaat agttaatagt
     1381 taatagttaa taataaatag ttaatattca ataaatgaag ttaatcaaag acctatattt
     1441 actacaaacc ggaagatgca tgttttcaaa ctctttttgg aaatgcaatt ttcaagcgaa
     1501 actccgtttc gcagaagtac tttcaaaagt aatcagcaaa aaaattcaaa atgcgtaact
     1561 tcaaataaga attaaaaaag ccgtgaagaa tttctccacg gctttctttt tttattaatc
     1621 tttaattccg gaatattaaa aagtttattt tcttcttact tgagtcctgt taacactgtt
     1681 aattttaact tcagaatttt tacttaaagg tgtagtcctc agcttatctc tttcacttct
     1741 ttgtttaagg acttcattga gttcagatgt tgaaacctgt ttgacctctg ttttaacatt
     1801 aatttcttta ctgattctga gagacatttc atatttattt ctctctgcat ctgatttgaa
     1861 aacaggatta atatttttat cctcagcagg tggcggtgct gatcccggag gtgcctgaat
     1921 agttacaatg ttaaatgcat tattgtcagc aattgcataa tcattgatat cggcaaaatt
     1981 atctccgttc acatctgaga ctatatatcc tccggcaaaa ttaaaagcgt cattgtcgat
     2041 tatcgagaga tcggcaattt caattacacc gtcctgatta cagtctccgc catacattcc
     2101 ccagagatta tttgttgagt tgatctgaaa ctggttatta ttgtaagcat aattttcttc
     2161 aagaaaatca taataaaaaa atccgcctct ggtatatgct tcaccgcctg atctgctcca
     2221 tgtctcaatg ctgtttctgt gctttaccac tatataataa tttcctgtaa gtgcttttga
     2281 gaaagtagga aaagtctcac aactatttga taaagtgata aaagcagaat caactttgat
     2341 attcgggaag tcagttctgt gtaaatatat atttacggaa tccggaattc ctgaatatat
     2401 acccggagta cctctgtaaa atccctgtgg tgtatttccg attccggcga tagttgcatt
     2461 tggcaatgtc ttcacacaca gattatctat atataaatta tttccatagc cgctctttgc
     2521 tttcagtcgg atcttatttg tacctgcagg taatgcataa atttttgaag cccattctga
     2581 gtttgtagga gtatatgcat ttacattcgg cggtgcagta tttagctcac ctgttccgtc
     2641 ttgtcttccg atcaatgtag ctaaagttgt atatgaacct cctgaattac ttgatgcttc
     2701 gacaagtaaa gtatccgggc caaagtttac atcggtataa ggtgcataag cttcatcaaa
     2761 tgtcagataa tcacctgtta aagttgcaac aaaattatct gatactattg attgattttc
     2821 attaaactga gcattgtaga aatcaaattt tgctgacccc gttccgatgc cgtatgccga
     2881 gacagtattt cttgaccagc ggtttgtacc ggtaaaatca attgtgagcg gcggtggcgg
     2941 gaaagtacct gatgtaaaat cttcacacgg tggtgcagac ggtccaccca cgatcgtaaa
     3001 gtttgtattc gaaatatcaa agaaaatatt tccgttagct tttactttta ttcttgcagt
     3061 ggctgtagct gtgtttggaa ttgtcactga ctgcgatccg tcatttggag tgtttgataa
     3121 aattgtagtc ggaaaagtat ttcctccgtc tgtggataat agaatatcaa cattcgcgca
     3181 attcaccggt gctgcagttg tatttgcaac attccatgta acagtctgag ctccggcatt
     3241 ccagctgaca ccggtattcg gtgaagtgac aaggaatggt cctgaactgt tgttcacgct
     3301 aaatgctaca gtgctgaaat ttattcctcc gccgcccgca ctgttatctc ttgcagttaa
     3361 tctgaaagtt aaacttcttg tatatgttgg taaaatttcg ccgatagttg aagtgttgtt
     3421 taacagatta cttaatttag gaaatgtcct ggtcggggaa acaaccggat taaatgatct
     3481 gaagatcggt gcatttccta caggagaatt aggtgcacct gcagcaccaa gatcaaattc
     3541 ttcccagcag tatgtcagtg aacctggagt ttcagtatca gtagctgatc ctgtaagttc
     3601 aaatggagta cttatcggaa ttgtaaatcc acctgaaggt acagtaactg ttggtggtgt
     3661 attacctgtg ttagtggtta caggacagga attcccgtta ccagtctgag taaaagctgt
     3721 catctcactt acacttcctg tatgaaaata atcatcactg tttgactgca gatcctgtgg
     3781 gctgcagata cctgcataag cctggatagt acttccgctc ccgggctccc aggctgtgga
     3841 accgtttctg ttaccacctg cgcagtttcc tgaactgcca ttgaatgtat gatttccacc
     3901 gaactgatgt cccatctcat gtgcgacata atctatataa aacgggtcac ctattggtga
     3961 tggcaatcct gttacaccgc gggctttact ggcattgaca caaacaactg aaagtcctgc
     4021 aactccgccg ccgccggtac tgaatacatg accgatatca taattagctg aaccgataac
     4081 agcattacaa tttgtctgat tttgtccgag cattgtcacg ccattattat ttgtgtatgg
     4141 atcggttcca ctgtttgtat aaactattaa attattattt gcaaccaatg tcattctcac
     4201 tgacaggtca cgctcataca cctgattcac tctgttaatt gcagttacaa ttcctgccag
     4261 tccaagcgct acagtaccac catgaaacgc cgtgtattca cctgtcgctg cacatgcaag
     4321 acgataagtc cttaactgtt gtccggtcat atcagaattg tgactgttta taagctcagt
     4381 gttttctgtt acttcagtca ccaaacattc aaatgtttga ttactaaagt aatccgattt
     4441 aaagtagctg atgtaatttt cagtattatc catactcaaa ggatcgataa aatagtctcc
     4501 gtttggtgtc agtaccattg catgaaaacc aaatgcagtg aaatcaattt tacctagtgc
     4561 ataaggatca tcaattcctt ttacattata agtcttcata tccggatact gatctgcaag
     4621 tccctgctcc atcatggaat attccgttac atagaatttt gacattccac catcaggatg
     4681 cggtaactcg attatcaatg gagatgcaac tgctctgtcc gtaaattcaa acggagctgc
     4741 tgtcattata tttctgaatg aattaagatt taaattcagg tatctgtatt tctgaggtat
     4801 tacttttgta atgtcattaa ttactatgtc cgctttgtct gtccagactg aattcgatac
     4861 agaattgtcc tgctgggcaa agacagattt tgtgtctgta gatattccat tttcacttat
     4921 acttaaattg aataagctga aaacgagaat tcccataaaa ataagttttc taattggtct
     4981 cattttttga ttagttaatt ggtttatgaa aaaacatttt tgaaattttt tttagataag
     5041 tataagtatg aaaaattaat attgggtctt tgaagtgtat actattaaaa acttttgatt
     5101 acattatagt aatttaattt ttaaaaaaaa atttaatttg ttatgtgtta taattttaat
     5161 cttgttctaa aagaatttta gatttattta ctattagttt gagaaataaa aaaagccgtg
     5221 aagaaaattc cccacggctt tttgattttt tatttttact taattttctc cgggaccttt
     5281 tccgctatta tattgaggag cggagtttat cggtggagtt tgattattta ttttaggttt
     5341 tactttatct ttgttatttt gtatttctga ttgtcttttt ttcaatactt cattcagctc
     5401 agttgaattc tttttatttt ctgtaactga attattatta aatttgcctg actttaaatg
     5461 aagttcatat ttagctttgg cttcctcatt ttcaaataca ggtgccgatt cttctaccgg
     5521 tggcaatggc tctgctcccg gtggctttga gactgtaata aaattattag cattgttatc
     5581 agcatatgca taatcagtaa tgtcaacaaa cagatctcca tttaaatctc cgattacata
     5641 accaacagta aaattagaaa cctgattgtc tataattgcc agatcagcta tatctataat
     5701 accgtcataa ttacaatcac cgctgaatac acctctataa aatggcaaca gacttacaat
     5761 tgcctgattg ttaccatatg cctgtccatc aggagtaaca aaattaaaat gagtatttga
     5821 tcctcttgaa taacctgtac cggtattact ccatgtctca agactgtttc tgtgttttac
     5881 tactttgtaa taattaccat ttaatgcttt ttcgaataac agatttaaaa cagcagagct
     5941 atttgtaaca gctaatgcag aatctactgc aatgttagga aaatcagttc tgtgaagata
     6001 tactcttact gtatcctgca atctccagaa aggagtattc ggaatgaaca tagcttcatt
     6061 taccagacca acggaattaa ttactgcagt aggcagagat tgaacacaaa tattatctac
     6121 atacaaatta ttaccaaaac cgctctttgc tcttagtctg atcttatttg ttccgacagg
     6181 aagtgcatat atctttgacg cccactgtga agatgttggt gtaaaagctg atgtggtagg
     6241 cggagctgta gttaactctc cggtgccgtc accatttcct gtcaaagttg ccagtactgt
     6301 ataactactg ccaaaattag ttgatgattc taccactaaa gtatcaggac caaatgccgg
     6361 agaatatgca gcatatgctt catcaaatgt cagataactt cctgccgcaa caggagcaaa
     6421 actttttgat aaaagtgact gaaccggtcc tacacctgca ttataaaagt caaactttgc
     6481 tgacccgttt ccgcttccgt atgctgatac attatttctt gaccatcgta aagttccggc
     6541 aaagtctata tcaatatcat caggcgggaa catagcacca gaaaaatctt cacacggaac
     6601 agctgataaa gttgagttaa cagttaccga tacagttctc ttatgtaccg gagttccatt
     6661 tgttcctgat cctgtgacag ttaccgtata tactccggga gttacagttc cgatagtcct
     6721 gatccgcata tctgcagttt ttggataagg agagcttaaa gtatttcctt ccggaaaatc
     6781 cgcgattaaa cttccggaac ccggtgttgg agtaattgaa gtctgaaata caacaatatc
     6841 tgtatataat tttactgcgg gtacattaac atcaatatat ctgaagtcat tggttgtgta
     6901 gattgttgtt tctgttggtg acaccgtcat agcataatca ggaaaataag caccgacatt
     6961 tttataagta ccggctcttc catcatacca tactgaaaat gatgtctttg gatttgcagt
     7021 cattccatca tagtctcctc tgtaacagtt tgtattcggt gcacaggctg gtgatggata
     7081 agtccatgat gcagttgtta atttttgatt tggcgcaaag gtagttccgc cgttatctgt
     7141 atacgttcca tatacattta ctgcatatgc agcaggattt tctctggtat cataccattt
     7201 tatataaagt cttccggttt ctttttcaca ccatatcgca ggaaaccact gatcgcttaa
     7261 ctgcggattt gcattatcat ttacagtgat cctgcttgac caggttaaac cctgattatc
     7321 tgaatactgt aaaataatat caggtttgtt tccgttaccg gcaggtacgt ttgatgcata
     7381 tactaaataa agtctgcctc tgtttggacc atagctatta tccatagcta tcattggata
     7441 aggtctggtt cttccgttat ttataacaag ccttccggca gtatttaaag taccaacata
     7501 accggctact gtcaggcttg accttactgc aaaagtagct ccgccgtcaa gagatctgtg
     7561 aaaagtatat gttacgttgg aagatgtacc tgtattagta acatacatta cacatccacc
     7621 ctgagtcgta ccgtttggac cgactgctat catgacacca ggtattgtat tgcttggtga
     7681 atatgtagtt gtccatgttg cattaccatt aatacttctg gaaaaattac cgggagtaat
     7741 agctgcatat aaataattct tgtaaggacc atttgtctga tctgctgcca gtgtgtttct
     7801 gtcatttccg tttacagaaa gtacaggtga accatatgtc tgaccgccgt ttgtagattt
     7861 ataaacatac tgatttgcca cacctgatcc gtaatacaaa ttacccagac tgtcaaaagc
     7921 tgaccagggg tcacagcatg tagacgcatg ataagcagga ttactagatg tccagtcatg
     7981 tccgttggta gtataataag cattctgacc gccgccgcca tttactccaa actgcatatt
     8041 catcggattt cttggatttg aaacaagcat ctgttcggca ttgtcagtac cgatctcaaa
     8101 attgtcaaaa cctgttccgt ctgtaattac atccagcaca tcatcatttt ccgtcatatg
     8161 cattctggat tcagaagttg gtctgtcaat gtacggatca gtttcatcat aatcataaaa
     8221 ttgttcggtg tgtttttgga aatcagtttt ttcatttccc tgaaatacct gcgtacctgc
     8281 taccaaaata aatacggcaa ctattgcaga ggtaatccat gttcgaagaa aagtcaactt
     8341 tttcatgtaa gttagtttag tttaaaaaaa ataatttttt tatttatgaa atagcaattt
     8401 aaaatatttc tcaaagcttc aaataattaa aacaaaagtt cttcaggata ttaaaagaat
     8461 acccaattat ctactgggaa attaatttcg gtgctttaat tatgtgggat tagatatgat
     8521 gtttaaaaaa gtattttttt actttttttt caagtcagtt ttataaaccc attcaataca
     8581 ttttcaaaga aaaattcaga cttgtatctc tcaatatatt atcaaaaaaa aagccgtgaa
     8641 gaaaattctc cacggctttt tgtattaata ttttaaatgt ttatttactt tgtccgaaag
     8701 atgatcctga tttaacagaa ttttctctga cattcgattt tacttcattt gtttttctga
     8761 caggtgctgt tctgttctga tctctttctt ttcttaatct cagaacttca ttcagctcat
     8821 attcggattt ctgattttta ttaacttctt cagaaagtcc tttttctctc attattttaa
     8881 gatgcatttc gtatttggtt ttttcagctt ctgttgtaaa tacaggatta tcattattta
     8941 cagtttcagg tgccggtgat gctcccggag gtgtttgctt tgtgacaata ttaaatgcat
     9001 tgttatcagc aagtgcatag tcagtcagat ctgcaaaatt atctccgtta agatctgaga
     9061 tcacatatcc tgctgcaaaa ttaaatgcat cattatcaat tgtcgcaaga tcacttaaat
     9121 ctataacacc atctttgttt acatcacctc catacaatcc ataatatggt gcaggatcta
     9181 taaatgcctg attattatta tatgcctgat ttaccgggtt aatgaaattg aaatttatag
     9241 aagatccacg ggtatatgat actccgcctg ctttactcca tgtctcgaga ctgttcctgt
     9301 gttttatttc taaataataa gttcctgtaa gtgctcttgt aaacggtgat aaagctacag
     9361 catttgtatt aagataggaa gttgcagaat ctactattat atttggaaaa tcagttctgt
     9421 gaagatatgc tctcacagtg tcagggattg tctgtggaaa tggtaatgct ctgtaaaatc
     9481 cttcaggtac taaaccaata tttgaagtag tggatggtgc cggaaagctt tgcacacaga
     9541 tgttatccag ataaagatta tttccaaatc cgctttctgc tctcagtctg actctgttgg
     9601 ttcctgcagg caatgaataa atttttgatg cccattgtga agcagtagga acaaaactgt
     9661 ttgtagttgc aggagctgta ttcagctcac cgcccgaagg aaatagtccc tgcaaaactg
     9721 ctagagttgt ataagaagaa ccgccgttta ctgatgattc tacgactaat gtatctggac
     9781 ctgggaaacc tgcgctgtat ggagcataag cttcatcaaa tgtcagataa tatcctgtag
     9841 gtacagcttc aaaatctgta ataagtgact gtggtgttct tacgttatca ctaaagaaat
     9901 cgtactttgc agaaccgctt cccactccgt aagcacttgc agcacttctc gtccagtatt
     9961 gagtacctga aaaattcaga tgaaaatatt ttggggtaaa tgccgaactt gtaaaatctt
    10021 cacagcctgt gcatggattc tttggcaata attcgatctt tgcagtataa gcctgatatt
    10081 ggaatccgcc aagtctgtta tccatccata gcggccatat cctctgatta ccatttacag
    10141 aatttccttc taccaaagaa gatgtcagtc cgatataatc accctgatat ccgccggcta
    10201 ctcccgataa agcaagggat gttggtctga atcttgcatc agatactttt aaatctgacc
    10261 atgtagaccc tccgtccata gatctggaca taaatacttc agcagtgtca gtatttgtag
    10321 ttccgcggtt gtcataatat actacgttca caccgcctga ttcatcgact cttacgacag
    10381 gaaaccactg tgactttccg ttgttcaaag catcctgatt tactttgatt ccggctgacc
    10441 atgtagttcc gccgtctgaa gatctgtgaa tcactatgtc tgcatcagtt cctgccggtg
    10501 ctaaattttt ctgtgaaaca acaatgtaga tccatcctgc tcttggtccg caggttctgt
    10561 ccacatcaat tctcgggaaa ctgtttactc tgatattagt ggaaagtaaa tttcctctga
    10621 taccattcat atcaaaaata ttgttattaa ctattgtcca gttgactccg ccgttagttg
    10681 attttgcaaa tcctgcatag tcttctgtaa atgaaccact ggtagtaggc tgtgcccagc
    10741 aaacgtaaac ttcaccattt ggtccggttc ggagatccac tccctgtgaa taatgacctg
    10801 ctggaggaac gttaatgtct gttgcagctg accagctgac tccgccgttt gttgtatatg
    10861 agaatgctat tggtggtgat gcagcattga atcttgacca ggcagtataa gatcttccgt
    10921 aatacggact cgtcggagaa tcatcggttg tagtaagatt tttatcctgt gaacctgttg
    10981 tgatagttac atcattagac cagttcagtc cgttgtttgt tgaaaaattt gctttgatac
    11041 cgccgccaag atgggaaatg ataaatcttc cgtctttgtc gatcgtaatt ccgggatcac
    11101 ctccgtgacc ggataaaatt gttccgacag tatttttcat tgtatcactt ccaaaccatg
    11161 tagtaccacc atcagtagta acataagctc cttcggaaat gaataacgga cctccagcat
    11221 tactgatagc attggatgaa ccgtacatta tcagcggatt accgggatgt cttgttatta
    11281 tcacttcagt ctgataactg aaaggtgacg ttcttggcaa aactctgaag ttcggattta
    11341 caacaagtac accgtttggt gtgttattgt acatcggttg attgtttgga acaaaatcat
    11401 tgtattccat tggtaaaggt gcagatattc tgttacccgg gtctaatgca ccggtcattc
    11461 ttgtatcctg actccatttt tcaggtttgc tgtcctgtga ttgtccggta aaaaaataga
    11521 aacctaaaac gatcagggga attgtgagga acaatttttt catgaatttt cctttcggga
    11581 aagtttaatt aaattattta tattacttag gtttaggaaa aataaaaaag caaaatcgct
    11641 tttttaatgc aagtcaaatt tattgattca caggtcagtt aacgtttctt accgacacaa
    11701 aattttacgt caaaaaattt tattccatat tttttctgat taaattttca aaattaaaat
    11761 ttaaaaattt aaaatttaaa attgaattaa tgatactgtt tcaataaatt gattttcata
    11821 aaaaacttat gccgcgaaaa tttattctgt catccatttt aattttttgt tctgtattct
    11881 taatttcaga attatctgta tcacaggatt actggatcag acagcagaca cctactacca
    11941 aacgtctcac aaattgtttt ttcataaata ataatacagg ctgggtaacc ggtgattcag
    12001 gtctcgttat gaaaagtact gactcaggtg taagctggca gcttcaaaac tcaaatactt
    12061 ttcacgatat tttttcagtt cattttataa atgacagact cggatgggct actgcctggg
    12121 ttttcaatcc cctgcctgat gagtttcccg gtactttact tcttaagact attaacggag
    12181 gaattaactg gacttcgcaa atgtatgctg acacgaattc ttattacgga tctttatttt
    12241 acctcgactc tctgaatgga tttctcggag gtatcccgca gtttattgtt tacactactg
    12301 ataatggagt ctcctggaat gacgctgttt cagattccat cggctttaac ggcttcccgg
    12361 taactttcat tacctttctg aatgcagata ccggatatgc atccggcggt gtgcaggaca
    12421 ttgccggtct cgtctggaag actgtgaata atgggaggaa ctggtcccct actgctgtcg
    12481 gaccggatcc aattaatgat atttattttt tttcggctga tacatctatc ggactttcag
    12541 gtgatttcaa attcggtgca agcttttacc ggacaaataa tgactggctg aattcattta
    12601 atgtgaatct aggctacatc ggaatggcgc agtctatcga tttcaggatt agaaatgacg
    12661 gatggataac tatcgggtat ttgagtaaac ttttcagatc tacaaacgcc ggagataact
    12721 gggtgaaaat ggatattcct gattcatctg cgatctttga tattcagttt attgactctg
    12781 cttacggatg gtgtgtcggt gatcatggcg cagtttataa atatgatatt atgacctctg
    12841 taaattcgaa tgaaatttcg aatgaaatta attcatttaa tttatttcaa aattttccaa
    12901 atccatttaa tcctgtcaca aagatcagtt atgaattaca aaatactgat aacggaatag
    12961 ttactctaaa ggtctttgat gttttgggaa atgaaattgc gactttggta aatgaaaaac
    13021 aaactccagg caggtacaat gtgactttcg acggcacagg actttcaagc ggtgtttact
    13081 tttacagact ttctttaaat ggaaatttca tttcgggaaa gactatgatg atgcttaaat
    13141 aaatttccgg cccgctcagg ttttcaaagt ttagagttga agtgaatcag gatgaaataa
    13201 tttactgcga tactttcagc ttctctgcta atttctcagc ctgccccctc agctcattta
    13261 ccgcagtagt ttcccaaatt tcacctttca gatttgatcc tattgtgaat aaatttttat
    13321 gagaattacc ttcggaattt atgatctgaa atgtatctgt ttcagtttta ataccaagct
    13381 ttaaattgtc ctgagatata attcctttta ctagacagtt ctttaaaaaa ctcttctcaa
    13441 gattcatcag atcagtttcg ggtccggtac agtttattac ccgggataca tttattgatt
    13501 ggacttcatt ttcttttttg tcaaaatatt ctacagttat ataattttca ttttctgtga
    13561 aatcaattat tttacctgaa ttaatatgca gctttccgtc aatccgtaat ttctgaattt
    13621 tatcgtgggt atgtaatggg attctgtgtc tcgcaacacc ccataaatga cgtaatcttg
    13681 acatgaataa ttttttatcc gcgtcagaaa aagatttcca gatcttttga gtgaaaggtc
    13741 tcagtgaatc gattaccggt tctgcagata caccgtattc ccttactgct tttatatgtt
    13801 tgttcacaag ttttagcaat tcataaagtc cggcattctc atctaattct tcagttaatt
    13861 ttgtgtattt caaaccatta tgtctgtgcg gcagaatgtt aaatccattt ggcgagatgg
    13921 aaaatatctc acctttaaat ccttgttcta acaaaccaag cacagtgtcc accatagtca
    13981 gtccattccc gattattaat acaggcagat cattatttaa ttcttttaca gaatcaattt
    14041 tccagggatt tcgaaaataa tttttactct gataaaattc cttattttta attgccggat
    14101 ttctcggaat attattaccc gtagtgatca cacaataatc caacagaaga attgaattgt
    14161 catctaaagt tattgaaaca gaattgtctg taacgtcaag atctactaca aatttattta
    14221 taactgtaat ttttatgtgt tttgattcgg ctgactctac tgcatttttc catatctcca
    14281 gaagatattc tccataatgc tctctcggta aaaatgaatt tgcaattaga tttctatctt
    14341 tatctgcata atcctttctt gaagtgaccc agtcaagaaa gtgatccggc ttatcaggat
    14401 aagcgctcat ttttcctgtt atgacattga gaatgtgctt acctgaatat gggcggtatg
    14461 caattccttt atttaaagtt tcactttcgt ttataagtat aatttctacc ggagcatttg
    14521 atttttcgat gagttgaacg gctgacattg tacctgaaaa cccgccgcca ataattccaa
    14581 tttttttcat ttaaaaaaag tataagtatt ttttttgact gcaaagctaa taaatataga
    14641 gataagtttc ccctcttata atttttgtct atccttttta aaatttattc tatatgaatt
    14701 tttcaaaatt ctcctttcat atccacatag ttattacctt ctccttctga cgccattgcc
    14761 cggtaagcag taagacgggg ttttcaaaat tataatacgt ttatgataat tcattgataa
    14821 aaatttgata aatatctaaa ttaataaaaa ttttatataa taaaatttac ccccaattaa
    14881 aattattgta ctaaatatga agacgtaacc ttccggtttt taatcttctt cagtaaataa
    14941 ttatattcac aataataata cgggttttat ttttacatat tcttttttta cccccggtat
    15001 aaaacgaatt attgattcat cagtataaat ctaattttaa aaaatttaaa tattttaaac
    15061 aataatcaga aaggatttta aatgaaaaaa cttattttac ttttcttatt tattttaatt
    15121 tcaacatatg catcctccca gactctggag gaaaaccttc agcaggttgg tccgaattat
    15181 ggtaagcttt atttacagcc gcttgtagat gcaatgggaa cagatgtaaa ttcaaatttc
    15241 ttttacacag catatgtacc ttacgacagt aaaaaacctg tgcaatttaa tattggttta
    15301 aggttcagag ctatgaatac ttttctctcc agtgtagatc aggtgtttga ttatacttat
    15361 tcggatacgt taactgtaaa cggagtacgt tattcaggag cttattcagt agaaaacgct
    15421 ccgactgtga tcggtaataa aacagaagct gtcgcaaagt tcacttccaa tggagtttat
    15481 tatccggaat ttgaccttga gctgataggt gggataataa atactacttt ggtaccattg
    15541 gtcattccgg aaattacatt tggaactgtt tatggaactg atgcttcaat tatacttctt
    15601 ccgactattg atttaaatga tctcggtaag ttcagaatgt tcggatttac actcagacat
    15661 aattttagtc attatgtcaa agattctcct gtagattatt caatcatggt cggatatcag
    15721 agaatgtcgc tgacagaaaa taataacaat gatctgtgga agtccaaaag cttttttata
    15781 aacggtcagt taagcaagac atttggcgga atatttacac catatatggc tatacagtat
    15841 gaacagttta aggcggatat ttcttatatt ttcaataact ttggccaggt ttttccggta
    15901 tctttcagcg tggacggagc aactaaattc aggggggtat tgggtgcaac agtaaaggca
    15961 ggattttttg cattcaatgt agatgctaac ctcggagaaa agttcgcact atcatgtgcc
    16021 tttaatttta taataatgta agagctggca ttgtcgccat tttttgacac catagagtgt
    16081 tcttcaccat tcgcttcaaa gctcactctt ttgaaagaac agagttagtg gcacagatta
    16141 ttaatctgtg taaaactcca aattaaatat tttctgaatt tttaaaacat gatctcttta
    16201 cataagaatt aaattgttta caaatcagca aataaaacaa ctacgaaatc ccatcaatat
    16261 cccaaaattc ataaaattct tacaattttc gacggctata ttttttaccc t
//
LOCUS       DAORVQ010000056        16292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1532_length_16292_cov_7.375008, whole genome shotgun sequence.
ACCESSION   DAORVQ010000056 DAORVQ010000000
VERSION     DAORVQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_16292_cov_7.375008"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLX80_06345"
     CDS             complement(<1..605)
                     /locus_tag="PLX80_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY20452.1"
                     /translation="MINLKSHNKGTTLFIIFLTIFIDLLGFGIILPLLPTFSVSVLHI
                     NEFTIGLMVGIFSLTQFLFTPVWGTLSDKYGRKRVLIISLAGSVVSNLLMALVFSGAL
                     MSVFLFIFARGFAGAFAGNISAAQAVISDVTPPEERSKGMGLISAAFSLGFVFGPTVG
                     GVLSESFGYSFPVFISAGLSLIALLLAIFVFKETLSIEIQM"
     gene            complement(602..1627)
                     /locus_tag="PLX80_06350"
     CDS             complement(602..1627)
                     /locus_tag="PLX80_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQY20453.1"
                     /translation="MKPYEQSEPVEPYREDIQNEVYSEYQEFNEEKKETGVWLNILLF
                     ILTFISTTLAGVYWLNLDPYRLEYFPLGLTYAFLILLVITTHEFGHYFAAKYHNVPVT
                     LPYYIPFPFIFLNPFGTMGAVIRMKARNYSRKALFDIGAAGPIAGWVTTLIILLIGFL
                     TLPSINYMYEIHPEYRATGIPVGGFSFGYNIIFWSFEKIFASSPDVFMPPMNEFYHYP
                     FLCVGWFGLLITSLNMMPVGQLDGGHISYAMFGDKHKYIAYATFALLLFFGLAGLLPL
                     MEININWGSINWLVWALLILFVIKIKHPPVFPEYDQPLSSGRMMVGWFTFFIFIVSFC
                     PVPVYEL"
     gene            1726..2706
                     /locus_tag="PLX80_06355"
     CDS             1726..2706
                     /locus_tag="PLX80_06355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQY20454.1"
                     /translation="MSQHLDNNSPEITDPAPQHKKGPLSKIPPLYFIFLSLALVFFLY
                     QIVGGLISYFMLGENMEINSDNLNVTRIVLTFAQFMFILAPAIVLEMLQDNNIKETFR
                     LKLPKTNLMVYSIIGLIFVQPFLQVFLYFQNELIFSLPFGTEFLNQLKEMFDMLESTT
                     VKLVTADSIPEFLLVILVIAITPAICEEFLFRGLVFKNFEKILPASKAIFFSGLLFAL
                     FHFHPFNLIPLIILGVYLTFIVYHSGSILTAVICHFLNNFISAMAVYIYGSETFSSLG
                     TEKSFSTDEKLQFLLLGIISLAAFLFVCKMIKRNSTAFSKELFSKDIINE"
     gene            2699..3499
                     /locus_tag="PLX80_06360"
     CDS             2699..3499
                     /locus_tag="PLX80_06360"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQY20455.1"
                     /translation="MSNLIKRIIAGIFGIPLLLYISYEGGYLLLGLTLILNTIAMWEF
                     CSMFEKKEIYTLKILTVAAADTLLVFSVNSSGISEQLILNILFCGILFITFEIFRKEK
                     RSPINPAISAFGLLYITVPFILMNFLQSQSVKLIIVFIFILIWTCDSMAYFGGRMFGK
                     RKLSDISPNKTWEGSVTGFIFTVIISVLLHFIFPNEITIYKAVSIGFLIGIFSQAGDL
                     FESMIKRYCGVKDSSNLIPGHGGILDRFDSLIFVVPVIFILLSVLKGF"
     gene            3658..4191
                     /locus_tag="PLX80_06365"
     CDS             3658..4191
                     /locus_tag="PLX80_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20456.1"
                     /translation="MTLKKSQNKDPKTSKKTSKTSNKVIEKLSEEDDDSLLNSDIIGD
                     KVIDDMLNNESLGELDVEDDEEFIHQVEEFKKEHSKSKLAKVYEYIGSPKFIELKKLS
                     KNDIKDELRKLIVALDKKNIIVHSHGDPDDREKYRFITEEIFNEDIEVKKNQNITFVY
                     EDYHPEMDDSEDDEEFF"
     gene            4207..4758
                     /locus_tag="PLX80_06370"
     CDS             4207..4758
                     /locus_tag="PLX80_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQY20457.1"
                     /translation="MAVQNNLSNLDTLIIPFSLEGTDGKFYSPSSFDDKKILIIIFIC
                     NHCPYVKAIMDRLVSFQNKYNDDGVRLVGINSNDPESYPEDSFDNMKLFYSEYKMNFP
                     YLCDETQDTARKYGAVCTPDFYLYDENRILKYRGRLDDNWKDETNITSKELEKATELL
                     LNDMKIDFQQIPSMGCSIKWKAD"
     gene            4885..5490
                     /locus_tag="PLX80_06375"
     CDS             4885..5490
                     /locus_tag="PLX80_06375"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HQY20458.1"
                     /translation="MPKLVIVRHGQSQWNLENRFTGWIDVDLSPKGIEEAERAAEILK
                     DHKFDKAYTSDLLRAQRTLDIILENTGMKNIPVEKDKALNERMYGDLQGLNKDECREK
                     FGEDQVKIWRRSFDTPPPNGESLKDTADRVLPYYKSKIEPELKAGKDIIISAHGNSLR
                     ALIMYLEGLSKEEILKTEIPTGSPKEYIFDDSLNVIETKYL"
     gene            5501..6136
                     /locus_tag="PLX80_06380"
     CDS             5501..6136
                     /locus_tag="PLX80_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQY20459.1"
                     /translation="MKQSIPRHKIKNIIFDLGNTLVFFDYDRFFDGLSALEENADKDK
                     LRKFFSDNDIDLKIGSCKLTIKDAYELLKKKFNLKTGYRKFLFLYCDIFNENTKMKDF
                     LENEMLNSNYNLYMLSNVDASHINFVNKNYPYVNHIKKRILSYKIKAVKPDKKIYKHL
                     IKKYKLEPKESLFIDDLKANIEESRSHGLNAIHYTSHDKFLKDFKKYTDEK"
     gene            6217..8502
                     /locus_tag="PLX80_06385"
     CDS             6217..8502
                     /locus_tag="PLX80_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQY20460.1"
                     /translation="MKLLTIIFTSAIILFGGISVCFDQSGNNLYAKTGSILWNDINES
                     NIDLRGERRIIPQVYRTVSTNFTELSTVLRSAPFEFTDRAKNDPMIIELPMPDGSMSK
                     FSITEYSMMEEGLAVKFPEIKTFNVKGIDDPYATGKLDITFHGFHAMILTPRGDYYID
                     PYSTNEKNIYISYFKKDFVSDQSYECMTEDDTDSFLKAKDHMQGGILSGSELRTYRLA
                     MAATGEYTTFHGGTVQSGLAAIVTAVNRVSGVYELEFAVRTILVANNNLLVYTNATTD
                     PYTNNNGFTMLGQNQSTIDQIIGPANYDVGHVFSTGGGGVAGLRVICINGVKANGVTG
                     LPSPIGDPFYIDYVAHEMGHQYGGNHSFNSQSSGCGGGNRNGNTAYEPGSGSTIMAYA
                     GICGSDNLQNNSDPYFHTISFLEIASYTQNSGGNTCAVITATGNTPPVITMPASGLII
                     PVSTPLQITGSATDAENQNSLTYCWEQYDLGPAGTLNNPSGNAPIIRSFRADTSSTRI
                     IPRISNLVNNTFATGEKLPTYARSLSFRLTVRDNNTAGGGVNYDYVSYTVTDAAGPFV
                     VSQPNTNVTWNANAAQTVTWDVANTNTAPVSVSNVNIKLSTDGGLTFPTMLLANTPND
                     GSQSVTLPFIDVTNARIKVESVGNIFFDISNTNFIISTQVSIVNNNSGIPSEYKLSQN
                     FPNPFNPTTKINFDIPENGSVSLKLMDISGKEVATLVKDNLNAGSYSFNFDGRGLASG
                     VYFYRLTTNNYSMTKRMMLVK"
     gene            8733..9584
                     /locus_tag="PLX80_06390"
     CDS             8733..9584
                     /locus_tag="PLX80_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQY20461.1"
                     /translation="MEWKGRDQSSNVDDRRRSTGKTVGGLGCLGTIIMVLLVWMLGGD
                     PLQFLQQMDIQPQQTQQQHIPSEAEDELAQFVSVVLKDTEDVWSKIFREKYGREYVKP
                     TLVMFTGSTQSECGFANSQTGPFYCPADQKLYIDLSFYQELKNKFKAPGDFAMAYVIA
                     HEVGHHVQNLLGIHNQVQNQRSRLSESEYNKLSVRVELQADFYAGVWANHAEEMKGIL
                     ETGDIDEALNAAAAIGDDRLQKQSQGHVVPDAFTHGTSEQRVRWFRKGFDTGDVTQGN
                     TFNTNNL"
     gene            9636..10379
                     /locus_tag="PLX80_06395"
     CDS             9636..10379
                     /locus_tag="PLX80_06395"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HQY20462.1"
                     /translation="MRSIILTLIFFAVSISASAQQMNITYKNEIDKNEKLNYMTDVTY
                     PQVDFGPEALMGVRGIAQDINTAINESVQNYIESFKTQLEEIKGDSKEGESNSFKITS
                     EAEITGGTLLSVKFTMFSNISGMAHPMTNDSSFNYSTISTGQLELKDLFREESEYLKF
                     ISAYSQKELEAYAYSQGNENIFDMIKLGTSPDANNFKVWNIKNDSLSITFNPYQAAPY
                     VFGIQEVKISLSEMLSMFDKTGPLEFLFR"
     gene            complement(10419..11264)
                     /locus_tag="PLX80_06400"
     CDS             complement(10419..11264)
                     /locus_tag="PLX80_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY20463.1"
                     /translation="MNHKAILPALGGFFFLALTSVFVKLISDFGTNTEWIVFIQYGTS
                     LLIITVITSFKKFKPIKTGQIKLHLVRGIAGMLGFTFFVICVRMTNIVDGTLLLNSAP
                     LFIPIIALIWLRKKMDPRIWFGIFTGFIGIIFIIHPVKGDLMQMGEIYGVASGIFLAV
                     ALIAMKELTRTETFEAVIFYFSLIAAICTLPFAIMNWVTPPAIVWVYGVSTGVAFMLY
                     LFLLQHAYKYEEAGKLAPFNYSAVVFTVIFNIVIFNSFPDLISIAGIVLVTAGGIMAI
                     IYHAK"
     gene            complement(11278..11832)
                     /locus_tag="PLX80_06405"
     CDS             complement(11278..11832)
                     /locus_tag="PLX80_06405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQY20464.1"
                     /translation="MSRIKEFNFTDRQGRDIHIRNAVPDDAEQINKIKRDIIDERYFM
                     LREPFEAKYTKEKTAEEIIYNTENEGALYIVAEHKSKVVGELDFINGSLFRTKHCGSF
                     TVFISKDFRDSGLGIQLIQALTAWAEKDPLIEKITLNAFSINIRALKLYEKCGFITEG
                     YCPKDMKLDDGTYIDSVLMYKFVK"
     gene            complement(11841..12515)
                     /locus_tag="PLX80_06410"
     CDS             complement(11841..12515)
                     /locus_tag="PLX80_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQY20465.1"
                     /translation="MKTGNFKVIIFDMDGVLIDSEPAYKTMNMAHFSSLGFKMNEEEY
                     NGYVGMSSLKMWSKIKSNYGLNENVEDLMASEKERMYKVLNSDLISEPIEGIKELIDS
                     FLKRNYRLCVASSSPKDNIKLVLKRHNLEMYFDFIVSGEEVTNGKPMPDIFLKAADHF
                     QSSPDNCFVIEDSSNGVTAAKAAGMKCIGFRNVNSGNQDISKADLIINSFSKTEINNV
                     LKFLYK"
     gene            complement(12662..14419)
                     /locus_tag="PLX80_06415"
     CDS             complement(12662..14419)
                     /locus_tag="PLX80_06415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY20466.1"
                     /translation="MHTAKFEDLNLSIEIQKAVKDMGFEEATPIQTQSIPVIMEGRDV
                     LGQAQTGTGKTASFAIPIIEKIRSDSKKIQAAVLCPTRELAIQVAEEFSELLKYKKNV
                     KVIPVYGGQSIDRQIKALRSGVQIIIATPGRLLDHINRKTVDLADVNFIVLDEADEML
                     DMGFRDDIETIIKSSSEDRQTVFFSATIPPEIAKLSKKYLVDPVHIKVIHKELTVPKV
                     TQYYLDIKTTGKLEVLTRIVDMYDPELTLVFCNTKRMVDDLVSHLQARGYLAEGLHGD
                     MKQMSRDRVMAKFRSRTLDILVATDVAARGIDVDEIDAVINYDMPQDEEYYVHRIGRT
                     ARAGREGHAFTFVRGKEFRKLKDIEKYTKTPINKLPIPTVKDVEEVRAKYTIEEISTV
                     IKTDKDFHQHDFIIENLLSENFTYEDIASALLKLHLGKNNEAEYVAIPEPSYKKYDDD
                     RSDRNKSRGKERRGERGKDRDRGRDRGDSKRIDRNDRNDRSDRRGDGGRKSYTDPDLK
                     RLFINIGKNAGIMPKDVLGAIAGEAGIPGSAVGEISIYDRYCFVNVDKKYSDKVVKIM
                     NNSQIKGNKIFVEYAADKK"
     gene            complement(14549..15886)
                     /locus_tag="PLX80_06420"
     CDS             complement(14549..15886)
                     /locus_tag="PLX80_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY20467.1"
                     /translation="MAKKSTKIFILDTNVILHDSSCILQFKENDIIIPLTVIEELDHF
                     KRGSQVINLNAREFARTLDSMTGSALFNGGVSLGKGRGKVRIAITKGLLNEIREMFID
                     DTPDHRILSTALEIHNKNKNRSVILVSKDVNLRMKAKAIGIPAEDYTTDMIGSVEELY
                     SGKEIIDDFDDEILMKLYQSPFEVPIEIIKKELNGELLPNKFYILRNNNRSVLAHYDP
                     IKEVMRKVDKIDIYGIKSRNAEQTFATDALINTEIPLVSMTGKAGTGKTLLAIASALQ
                     VRKNYRQIFIARPVVPLSNKDIGYLPGDIEDKLAPYMQPLWDNIKVIQDQYSETDKQH
                     QTISQLIKDQKLVIEPLSYIRGRSLQRIFFIVDEAQNLTPHEIKTIITRAGEGSKIVF
                     TGDVYQIDHPYLDSQSNGLSYLIEHFKGQKLYAHVNLLKGERSELAELASNLL"
BASE COUNT         5379 a         2837 c         2805 g         5271 t
ORIGIN      
        1 ttcatttgaa tttctatcga tagagtttct ttaaaaacaa aaattgccag tagtaaagct
       61 attaaggaaa gtcctgcgga aataaataca ggaaaactat atccaaaact ttctgaaagt
      121 actccgccta ctgttggccc aaatacaaat cccaaagaaa atgcagcact gatcagaccc
      181 attccttttg atctttcctc aggcggtgtt acatctgaaa tgactgcctg agctgctgag
      241 atatttccag cgaatgctcc tgcaaatcct cttgcaaaaa taaatagaaa tactgacatc
      301 aaagcaccgg aaaatacaag tgccatcaaa agatttgaaa cgacgctgcc tgctaaactt
      361 atgatcagta cacgcttcct gccatattta tctgaaagag tcccccaaac aggtgtaaat
      421 aaaaactgag taagcgaaaa tattccaacc atcagtccaa tcgtaaattc attaatgtga
      481 agtacgctta cagagaaagt cggcaataat ggaagaatta ttccgaagcc aagcagatca
      541 ataaatattg taaggaaaat tataaaaagg gtggttccct tgttgtgaga tttaagattg
      601 atcataactc ataaaccggt accggacaaa acgaaacaat gaatataaaa aatgtaaacc
      661 agccgaccat cattctgccg ctgctgagcg gctgatcata ttcaggaaaa acgggagggt
      721 gtttaatttt aattacaaac aatataagca aagcccagac aagccagttg attgaacccc
      781 agtttatatt gatctccata agcggaagta gccctgcaag tccgaagaaa agtaataatg
      841 caaaagtagc ataagcaatg tatttatgtt tgtcgccaaa catcgcatag gaaatatgtc
      901 cgccatcaag ctgtccgacc ggcatcatat ttagagaagt tatcagaagc ccaaaccatc
      961 cgacgcacag gaatgggtaa tgataaaatt cattcattgg aggcatgaaa acatcaggtg
     1021 acgatgcaaa tattttttca aaagaccaaa atattatatt atatccgaaa gaaaatccgc
     1081 cgactggaat tcctgttgct ctgtattcag gatgaatttc gtacatataa tttatcgacg
     1141 gcagtgtaag aaatccgata agtaaaatta ttaaagtcgt gacccaaccg gcgattggtc
     1201 cggcagctcc aatatcaaat aaagcttttc gactataatt ccttgccttc atccgaatga
     1261 ccgctcccat tgttccaaat ggatttaaaa aaataaaagg aaaaggaata taataaggta
     1321 aagtaacagg aacgttatgg tacttcgctg caaagtagtg accgaattca tgtgttgtga
     1381 tcacaagtaa gatcagaaat gcatatgtaa gacccagtgg aaaatattca agtcgatacg
     1441 gatccagatt cagccagtat acaccggcta atgtagtcga gataaaagta agtatgaata
     1501 aaagaatatt taaccataca cctgtttcct ttttttcttc gttgaattcc tgatattctg
     1561 aataaacttc attctgtatg tcttcccggt atggctcgac aggttctgat tgttcgtaag
     1621 gtttcattga tgcaaaataa ttttaatgaa tattaaattc aatctatctt tactctttaa
     1681 ttaaatatct gtttttatga tattgcaaaa tatcaaatca taaatttgtc acagcacttg
     1741 gataataatt ctcctgaaat tacagacccg gcacctcagc ataaaaaagg accgttgtca
     1801 aagatccctc cactatattt tatattttta tctctcgcac tggtattttt tctttatcag
     1861 atcgtagggg gattgatctc ctatttcatg ctcggtgaaa atatggagat caatagtgat
     1921 aatctcaatg taacaaggat cgtacttaca tttgctcagt tcatgtttat acttgcacct
     1981 gccatagtgc ttgagatgct gcaggataat aatattaagg aaaccttcag actgaaatta
     2041 ccgaaaacaa atttaatggt ttattctatt ataggtttga tatttgtaca gccgtttctt
     2101 caggtgtttt tatattttca gaatgaactg atattctcac ttccatttgg aactgagttt
     2161 ttaaatcagc tgaaagaaat gtttgatatg cttgaatcca ctacggtaaa gcttgtcact
     2221 gcagattcaa ttccggaatt tctgttagta attttggtaa ttgcaattac acccgctatc
     2281 tgtgaggagt ttcttttcag aggtctggta tttaaaaatt ttgaaaagat actgccggct
     2341 tcaaaagcca tatttttttc aggactgctt ttcgctttgt ttcattttca tccttttaat
     2401 ctgatcccgc ttataatttt aggtgtatat cttactttta tagtttatca ttcgggaagt
     2461 attctgacag cggtgatctg tcattttctg aataatttca tttcagcaat ggcagtttat
     2521 atatacggat cagagacatt ctcatctttg ggaacggaaa aatctttcag cacagatgag
     2581 aagctgcagt ttcttttatt gggaattatc tctcttgctg cattcttatt tgtttgtaaa
     2641 atgatcaaaa gaaattcaac agcattttcg aaagaacttt tttcaaaaga tataattaat
     2701 gagtaatctc ataaaaagaa ttatcgcagg aatttttgga atacctttgc tactttatat
     2761 ttcatatgaa ggaggatatt tacttcttgg acttactttg attcttaata caattgctat
     2821 gtgggaattc tgttcaatgt ttgaaaaaaa agagatttac acattaaaaa tattaacagt
     2881 ggcagccgca gatacactgc ttgtattctc tgtaaactca tccggaattt cagaacaatt
     2941 aattttaaac attttatttt gcggaatact ttttattact tttgaaattt tcagaaaaga
     3001 aaaacggagt ccaataaatc cagccatatc agcttttggt ctgttgtaca ttacagtgcc
     3061 gtttatactt atgaattttc ttcagtcaca atcagtaaag ctgatcattg tatttatttt
     3121 tattttaata tggacttgtg attcgatggc atatttcgga gggagaatgt ttggtaagcg
     3181 taagctcagc gatatcagcc caaataaaac atgggaagga tctgtaaccg gatttatttt
     3241 tactgttata atttcagtgc tgcttcattt tatttttcca aatgaaataa ctatttataa
     3301 agctgtgtct attggatttc tgataggaat attctctcag gcaggagatc tttttgaatc
     3361 aatgataaaa agatattgtg gagtaaaaga ttcttcaaac ctgatccccg gacatggcgg
     3421 tatattagac agattcgaca gcctgatctt tgtggttcct gtaattttta ttttactttc
     3481 ggttttaaaa gggttctgaa aatataaatt tcaaataaat ttttgtatct tgaaaatgta
     3541 aagaagataa aatccattta aatattttat aattaattta ttattgataa attgaattat
     3601 tattataaaa ttaattaaac aaaaaaatca ataaaccaac ttagcgaaac aagaataatg
     3661 actttaaaga aatctcaaaa taaagatccg aagacttcaa aaaaaacttc caaaacttct
     3721 aacaaggtga tagagaaatt atctgaagaa gatgacgata gccttttaaa ctcagacatt
     3781 atcggagata aagtaattga cgatatgctg aataacgaat cgcttggtga acttgatgtt
     3841 gaggacgatg aagaatttat acatcaggta gaagagttta aaaaagaaca ttccaaaagt
     3901 aaattagcta aagtctatga atacatcggt tcaccaaaat tcattgaact aaaaaaactc
     3961 agcaagaatg atatcaaaga tgaattgaga aagttaatag tagctttaga taaaaaaaat
     4021 attatcgttc attcacacgg agatcctgac gacagagaaa aatacagatt cataaccgag
     4081 gaaattttta atgaagatat agaagtaaag aaaaatcaga acataacttt tgtctatgaa
     4141 gattatcatc cggagatgga tgacagcgaa gacgatgaag aattttttta atatctaata
     4201 tataatttgg cagttcaaaa caatcttagc aatcttgaca ctctaattat tccgttttcg
     4261 cttgaaggaa ctgacggaaa attttattcc ccttcttctt ttgacgataa aaaaattctc
     4321 ataattattt ttatttgtaa tcattgtccg tatgtaaaag ctattatgga cagacttgtt
     4381 tcttttcaga ataagtataa tgatgatggt gttcggcttg ttggaataaa ttccaatgat
     4441 ccggaatcat atccggaaga ttcttttgat aatatgaaat tgttttattc tgaatataaa
     4501 atgaatttcc cgtatctgtg tgatgaaact caggatactg caagaaaata tggagcagtc
     4561 tgcacacctg atttttattt gtatgatgaa aatagaattt taaaatacag aggacggctt
     4621 gatgacaact ggaaagatga aactaatatc acttcaaaag aacttgaaaa agctacagaa
     4681 ttattactca atgatatgaa aatagatttt cagcagatac cttctatggg ctgttcgata
     4741 aagtggaaag ctgactgagt atataacaat tgtttaaacc tccttattca aatcacaaat
     4801 ccaatattca attctgtttt aaattgtaag aaaaatttat ttaaactaat ttgttagatt
     4861 cttaattaat aaaaattact ttcaatgccc aagttagtta tagtcagaca cggacagtca
     4921 caatggaatc ttgaaaaccg attcactggg tggatagatg ttgatctttc accaaaagga
     4981 attgaggaag cagaaagagc tgctgaaatt ttaaaagatc ataaatttga taaagcatat
     5041 acatcagatc tgcttagagc acagaggaca cttgatatta ttttagagaa taccggtatg
     5101 aaaaatattc ctgttgaaaa agataaagct cttaacgaga gaatgtacgg ggatctccag
     5161 ggactgaaca aagatgaatg cagggaaaag tttggagaag atcaggtaaa gatctggaga
     5221 agaagttttg atacaccacc tcccaacggt gaaagtctca aagatactgc tgacagagta
     5281 ctgccttatt ataaatcaaa gatagaaccc gaattaaaag caggaaaaga tataattatt
     5341 tctgcgcatg gaaatagtct gagagcactc attatgtatc tggaaggtct tagcaaagaa
     5401 gagatcctaa agacagaaat tccaacaggc tcaccaaaag aatatatttt tgatgattcg
     5461 ttgaacgtta ttgaaacaaa atatctttaa ctaatattta ttgaaacagt ctatcccccg
     5521 acataaaatt aaaaatatta tattcgacct gggaaacacg cttgtgtttt ttgattatga
     5581 ccgattcttt gatggattat cagctcttga ggagaatgca gataaagata aactcaggaa
     5641 gtttttttct gataatgata tagatctgaa aatcggaagc tgtaaactta caattaaaga
     5701 tgcttatgaa ttacttaaga aaaaattcaa tcttaaaacc ggttacagaa aatttctttt
     5761 tttatattgc gatattttta atgagaatac aaaaatgaaa gactttcttg aaaatgaaat
     5821 gttaaacagt aattataatt tatacatgct ttcaaatgta gatgcttcac acataaattt
     5881 tgtaaacaag aattatcctt atgtaaatca cataaagaaa agaattcttt catataaaat
     5941 aaaagcagtt aaacccgata agaaaattta taagcattta attaaaaaat acaaactcga
     6001 acccaaagaa tctttattta tagatgatct gaaagcaaat atagaggaat caagatctca
     6061 cggactgaat gcaattcatt acacatctca tgataaattt ctaaaggatt ttaaaaaata
     6121 cactgacgaa aaataactgt gtgtaaaaat attctcataa tatcaaaaca tctaattaca
     6181 aataacaaac atctttttaa acataaacca attaacatga aacttctgac aattatcttt
     6241 acttcagcta ttattctctt tgggggaatt agcgtttgtt ttgatcagtc cggaaacaat
     6301 ctgtatgcaa agacaggttc aatactctgg aatgatataa atgaaagcaa cattgacctg
     6361 agaggagaga gacgaataat tccacaggtt tacagaacag tgagtacaaa ttttactgag
     6421 ctaagtacag tgctcaggtc agcgccattt gaatttacag acagagcaaa gaatgatccg
     6481 atgatcattg agcttccaat gcctgacggc agcatgtcaa aattttccat cactgaatat
     6541 tctatgatgg aagaaggtct tgcagtgaaa tttcctgaga taaaaacatt taatgtaaaa
     6601 ggaattgatg acccgtatgc taccggaaaa cttgatatta cttttcatgg atttcatgca
     6661 atgatattaa ctccaagagg tgattattat attgatccat acagtactaa tgaaaaaaat
     6721 atttacataa gttatttcaa aaaagatttt gtttctgatc agtcttacga gtgtatgacg
     6781 gaagatgata ctgacagttt tctgaaagca aaagatcata tgcagggtgg aattctttca
     6841 ggttcggaat taagaacata cagactggca atggcagcga ccggtgaata caccacattt
     6901 catggaggaa ctgtccagtc gggactggct gcgatcgtaa ctgcagtgaa cagagtatcg
     6961 ggtgtttacg aacttgagtt tgcagtaagg acaattttag ttgcaaacaa taatctgctt
     7021 gtatatacaa atgcaacaac agatccatat acaaataata atggctttac aatgcttggg
     7081 cagaatcagt caactattga tcaaataata ggaccagcaa attatgacgt cggtcatgta
     7141 ttcagtactg gcggcggcgg agttgccgga ttaagagtga tctgtattaa cggagttaaa
     7201 gcaaatggtg tcaccggtct gccttcacct atcggagatc cgttttatat tgattatgta
     7261 gcacatgaaa tgggacatca gtatggtggt aatcattcat ttaacagtca gtcaagcgga
     7321 tgcggcggcg gtaacaggaa cggcaatacg gcatacgaac ccggcagtgg aagcactatc
     7381 atggcatatg caggaatttg cggttctgat aatttacaaa ataacagtga tccgtatttt
     7441 cacacaataa gttttttaga aatagcatcg tacactcaaa acagcggtgg taatacatgt
     7501 gctgtaatca ctgctacagg aaatacacct cctgtaatta ctatgccggc aagtggtctg
     7561 ataattccgg tcagtactcc gcttcagatc acaggaagtg caactgatgc agaaaatcag
     7621 aattcactta cttactgctg ggaacaatac gatctcggac ctgctggtac actgaataat
     7681 ccatctggta atgcaccgat cataagatca ttcagagctg atacatcttc tacaagaatt
     7741 attccaagaa taagtaattt agtaaacaac acttttgcaa ccggagaaaa gcttccgacc
     7801 tatgcaagat ctctgagttt cagattaaca gtgagagata ataatacagc aggcggcggt
     7861 gtaaattatg attatgtcag ctatacagta actgatgctg cgggaccgtt tgtcgtttca
     7921 cagccaaata caaatgtcac ttggaatgca aacgcagcac agacagtaac ttgggatgta
     7981 gcaaatacaa atacagcacc ggtcagtgta tctaatgtta atataaaatt atctactgat
     8041 ggcggattga ctttcccgac aatgcttctt gcaaacacac caaatgacgg aagtcagtct
     8101 gtaactcttc ctttcattga tgttaccaat gcaaggatca aagtagaatc agtcggaaat
     8161 attttctttg atatctcaaa tacaaatttt ataatctcta ctcaggtatc aatagtaaac
     8221 aacaattcag gaattccttc tgaatataag ctttcacaga attttccaaa tccgtttaac
     8281 ccaacaacaa aaattaattt cgacatccct gaaaatggta gtgtttcttt aaaacttatg
     8341 gacatttccg gaaaagaagt tgcaacactt gttaaagata atctcaatgc agggtcatac
     8401 agttttaatt ttgacggcag aggactggcg agtggagttt atttttacag acttaccaca
     8461 aataattatt ctatgacaaa gagaatgatg cttgtaaaat aatgttcaat gtgtaatgtg
     8521 taatgtgtaa tgtacaatgt atagaattta ttatttaaaa tttaatattt aatattaaaa
     8581 aggcagtttg caaatgcaga ctgtcttttt tttattgtga caaatatttt gaatggtctt
     8641 tgaatttttt gcatctaaat taacattttg aataaaaatt atttaaacta attttagcaa
     8701 atcaataacc ataaaaattt aaattttata ttatggaatg gaaaggccgg gatcaaagtt
     8761 cgaatgtaga tgaccgcaga aggagcaccg gaaaaacagt cggcggttta gggtgtcttg
     8821 gaacaataat aatggtactg ttagtatgga tgcttggcgg agatcctctg cagtttttac
     8881 agcaaatgga tatacagcct cagcagacac agcagcagca tataccttct gaagcagaag
     8941 atgaactggc gcagtttgtt tctgtagttc ttaaagatac tgaagatgta tggagtaaaa
     9001 tattcagaga gaaatacggc agagaatatg taaaaccgac ccttgtgatg tttacaggtt
     9061 cgacacagtc cgaatgtggg tttgcaaatt cacagaccgg acctttctat tgtccggcag
     9121 atcagaagtt atatattgat ctgagtttct atcaggagct aaaaaataag ttcaaagcac
     9181 ccggtgattt tgcaatggct tatgtaatag cacatgaagt cggacatcat gtacagaatc
     9241 ttctcggtat tcataatcag gtacaaaacc aacgaagcag attatctgaa tctgaatata
     9301 ataaattgag tgtgagagta gaattacagg cggattttta tgcaggtgta tgggcaaatc
     9361 atgctgaaga aatgaaagga attctcgaga ctggagatat agatgaagca ttaaatgcag
     9421 cagcagctat cggcgatgac cgtttacaaa agcagtctca gggacatgta gtgccggatg
     9481 catttactca cggtacttcc gagcagagag ttagatggtt tagaaaagga tttgatactg
     9541 gtgatgtcac tcagggaaat acatttaaca ccaataatct ataagcaaaa aacagacaga
     9601 atttaattaa cacataattt tttaaaatca agactttgag atcaataata cttacattaa
     9661 tttttttcgc tgttagtata tctgcttcag ctcagcaaat gaatataaca tacaaaaatg
     9721 aaattgataa gaatgagaaa ttaaattaca tgacggacgt gacgtatcct caggtagatt
     9781 ttggtccgga agctttgatg ggagtgagag gaattgcaca ggatattaat actgcaatta
     9841 atgagtctgt acagaattat atagaaagtt ttaaaacaca gttagaggaa attaaaggtg
     9901 attcaaaaga aggtgaatca aattctttta agattacatc agaagctgaa attacaggtg
     9961 gtactttatt atcggttaag ttcacaatgt tcagtaatat ctcaggtatg gcgcatccta
    10021 tgacaaatga ttcttcattt aattacagta cgatctcaac gggtcagcta gagttaaaag
    10081 atctgttcag agaagaatca gaatatctga aattcatttc ggcttattct cagaaagagc
    10141 ttgaagcata tgcttattca caggggaatg aaaatatttt tgatatgata aaacttggta
    10201 cttcacccga tgcaaataat ttcaaagtct ggaatataaa aaacgatagt ctgagtatta
    10261 catttaatcc ttatcaggca gcgccgtatg tgtttggtat tcaggaagta aagatttcct
    10321 taagtgaaat gttgagtatg tttgataaga caggaccatt agaatttttg ttcagataaa
    10381 atcttttcca aaaataaaaa tcaatttaaa atattagatt attttgcatg ataaatgatc
    10441 gccattatcc cgcctgcagt aacaaggaca attccagcta ttgagatcag atcaggaaaa
    10501 ctgttaaaaa ttacaatatt aaatatcact gtaaatacta cagctgaata attaaatggt
    10561 gcaagcttac ctgcttcttc atatttgtaa gcatgctgca gtaaaaacag atataacata
    10621 aatgctactc ctgttgaaac tccgtaaacc cagactatag ccggtggtgt gacccagttc
    10681 attattgcaa atggtagtgt acatattgca gcaatcaatg aaaaatagaa tataactgct
    10741 tcaaaagttt ctgttcttgt taattctttc atagctatca gagctacagc aaggaaaatt
    10801 cctgaagcta ctccgtatat ttctcccatc tgcatgagat caccctttac aggatgtata
    10861 ataaatatta ttcctataaa tcctgtaaaa attccaaacc agattctcgg atccattttt
    10921 tttcttaacc atatcaatgc gattatggga atgaaaagag gtgcagaatt cagaagcaac
    10981 gttccatcca caatgtttgt cattctcaca cagatcacaa agaaagtaaa accaagcatt
    11041 cccgctattc ccctgacaag atgaagtttt atctgaccgg ttttaatggg tttgaatttt
    11101 ttaaaagatg tgatcacagt aattatcaaa agtgaagtgc catactgaat aaaaactatc
    11161 cattcagtat ttgtcccgaa atccgatatc agtttaacaa acacactggt aagagcaaga
    11221 aagaaaaagc ctccaagcgc gggcaggatt gctttatgat tcattaaatt ttgataatta
    11281 ttttacaaat ttatacataa gtacgctgtc aatataagtg ccgtcatcaa gcttcatatc
    11341 ttttggacaa taaccctctg taataaatcc gcatttctca tataatttta aagcgcgtat
    11401 atttatggaa aatgcattca aagtaatttt ttctattagt ggatcttttt ccgcccaggc
    11461 tgtcagcgct tgtattaatt gaatacctaa acctgaatcc ctgaagtctt tcgaaatgaa
    11521 gacagtaaaa gatccgcagt gctttgtacg gaataatgaa ccgttaatga aatcaagttc
    11581 acctacgacc tttgatttat gttcagctac aatgtaaagc gctccttcat tttcagtatt
    11641 ataaattatt tcttcagcag ttttttcttt tgtatatttt gcctcaaaag gttcacgcag
    11701 catgaagtat ctttcatcaa ttatatctct tttaatttta ttgatctgct cagcatcatc
    11761 cggcactgca tttctgatat gtatgtctct gccttgtctg tctgtaaaat taaattcttt
    11821 gatccgtgac attataaaaa ttatttatat aaaaatttca aaacattatt aatttcagtt
    11881 ttagagaaac tgttaattat cagatcagct ttcgaaatat cctgattacc cgaatttaca
    11941 tttctgaaac caatacattt cataccggca gcttttgcag cagttactcc gtttgaggaa
    12001 tcttctatta caaaacaatt atccggtgat gattgaaaat ggtcagcagc tttgagaaaa
    12061 atatcgggca tgggttttcc gttagttact tcttctccgc taacaataaa atcaaaatac
    12121 atttctaaat tatgtctctt caaaactaat tttatattat cttttggtga agatgaagct
    12181 acacaaagtc tgtaatttct tttaagaaaa gaatctataa gttctttaat accttcaatg
    12241 ggttcagaga taagatccga attcaacact ttatacatcc tctctttttc tgaagccatt
    12301 aagtcctcca cattttcatt caatccataa ttgcttttta tcttactcca catttttaat
    12361 gaagacatcc caacataacc attgtattct tcctcattca ttttaaaacc aagtgaagaa
    12421 aaatgcgcca tgttcattgt cttatatgct ggctcggagt caattaagac tccgtccatg
    12481 tcaaatatta ttactttgaa attaccggtc ttcaactaat tactcctaag taaaagcaga
    12541 gtcacgtgtt tttcggactc tgcgggatgt tgataataaa aattgattac agttattgta
    12601 acggcagagt cacgtgtact tcggactctg cgggatgttg ataataaaac tgattgcagc
    12661 tttatttttt atcagcagcg tattctacaa atattttatt tcctttaatc tgactgttgt
    12721 tcatgatctt gacgaccttg tcagaatatt ttttatctac atttacaaaa cagtatctgt
    12781 catatattga gatctcacca accgctgatc cgggaatgcc tgcctctcct gctattgcac
    12841 ccaatacatc tttaggcatt ataccggcat tttttccaat gtttatgaaa agtcttttaa
    12901 gatcgggatc agtataactt tttctgcctc cgtctcctct tctatcactt ctatcatttc
    12961 tgtcatttct gtcaattctt ttgctgtcac cccggtctct gcctctgtct ctgtccttac
    13021 cgcgttctcc tcttctttct ttaccgcgtg atttgtttct atcggaacga tcatcatcat
    13081 atttcttata agaaggttca ggaattgcaa catattcagc ttcattattt ttacctaaat
    13141 gtaatttcaa caaagctgat gcaatgtcct catatgtaaa attctcactt aaaagatttt
    13201 ctataataaa gtcatgctga tgaaaatctt tgtctgtttt tatgactgtc gaaatttctt
    13261 cgatcgtata ttttgctctt acctcttcta catcttttac tgtaggtatc ggtaatttat
    13321 ttatcggtgt ctttgtatat ttttcaatat cttttagttt tctgaattcc tttcctctta
    13381 caaaagtaaa tgcatgtcct tctctgccgg ctcttgcagt tcttcctatc ctgtgtacat
    13441 aatattcttc atcctgaggc atatcataat ttataactgc atctatttca tctacatcaa
    13501 ttccccttgc tgctacatct gtagctacaa gaatgtccaa cgttctgctt ctgaattttg
    13561 ccattaccct gtcacggctc atctgcttca tgtccccgtg aagaccttct gccagatatc
    13621 ctctcgcctg aagatgtgat acaagatcat ccaccatccg cttagtatta caaaatacca
    13681 gcgtcagctc aggatcatac atgtcaacaa ttcttgtaag cacttcaagc tttcctgtcg
    13741 tcttaatatc taaataatac tgtgtcactt ttggaactgt cagctcttta tgaatcactt
    13801 ttatatgtac aggatcgact agatattttt tggaaagctt tgcaatttca ggtggaatgg
    13861 tcgcagagaa aaatactgtc tgcctgtctt ctgaagatga tttaattata gtttcaatat
    13921 catcacgaaa tcccatgtca agcatttcat cagcttcatc aagtacaatg aaattaacat
    13981 cagcaagatc aacagtcttt ctgtttatat gatcaagaag tctgcccggt gtagcgatga
    14041 tgatctgaac tcctgatctc aaagctttga tctgtctgtc aattgactga cctccataaa
    14101 caggaatgac ctttacattt tttttgtatt taagtaattc actgaattct tcagcaacct
    14161 gaatggcaag ttcccttgta ggacaaagta ctgcagcctg tatttttttg gaatccgatc
    14221 ttattttttc tataattgga attgcaaagg aagctgtctt tcctgtgcct gtctgcgcct
    14281 gtcccagtac gtcccttcct tccataatta caggaatgga ttgtgtctga ataggagtcg
    14341 cttcttcgaa ccccatgtct tttacggctt tctgaatttc tatagataaa ttcagatctt
    14401 caaattttgc ggtatgcatt tattgtgttt gattttttaa aattatgtag atgtataata
    14461 agttttatat tgtaatgttg tgggttcatt agttggtaac acgaaatttc ccggatcata
    14521 acaacactat aatagacaaa atttttattt aaagcaaatt gctggccagc tctgcaagtt
    14581 cagatctttc gccttttaat aaatttacat gagcgtaaag tttttgacct ttaaaatgtt
    14641 ctatcaaata cgaaagtccg ttggactgtg aatcaagata cggatggtcg atctgatata
    14701 catcaccggt aaatacaatc tttgaacctt caccagccct tgtaataatt gttttgatct
    14761 catgcggagt aaggttttgc gcttcatcca ctatgaaaaa aattctctga agacttcttc
    14821 cccgaatgta actaagcggt tctatcacaa gcttctgatc ttttataagt tgtgatattg
    14881 tctgatgctg tttatccgtt tcagaatact gatcctggat cactttgata ttgtcccaaa
    14941 gcggctgcat gtatggtgca agtttgtctt caatgtcgcc cggcagataa cctatatctt
    15001 tgttgctcaa tggcacaaca ggtctagcaa taaaaatctg acggtaattt tttctcacct
    15061 gcaatgcaga tgcaatcgcc agtaaagtct ttcctgtacc ggcttttcct gtcattgaga
    15121 ccagtggtat ttcagtattg atcaatgcat ctgttgcaaa tgtttgttca gcgtttcgag
    15181 attttattcc ataaatatcg atcttatcaa ctttcctcat tacttctttt atcggatcat
    15241 aatgcgcaag tactgatctg ttgttgtttc taagtatata aaatttattc ggcagtagtt
    15301 caccgttgag ttctttttta ataatttcaa ttggcacttc aaacggcgac tgataaagct
    15361 tcatcaagat ctcatcatca aaatcatcta tgatctcctt accgctgtaa agctcttcta
    15421 cactgccgat catgtcagtc gtataatctt cagccggtat gccgattgct tttgccttca
    15481 tccggagatt cacatcttta ctgacaagaa tcacagagcg gttcttgttc ttattgtgaa
    15541 tttcaagagc cgtgcttaat atcctgtgat cgggagtatc gtctataaac atttccctga
    15601 tctcattcag tagaccttta gtaattgcga tacggacttt tcctctgcct ttaccgagcg
    15661 atacaccccc gttgaataaa gccgatccgg tcatcgaatc cagtgtccgt gcaaactcac
    15721 gagcattcag attaattacc tgactgcctc gcttgaaatg atcaagctcc tctatgactg
    15781 tgagaggaat gataatgtcg ttttccttaa attgtaaaat gcatgaagag tcatgcagga
    15841 ttacgtttgt atccagtatg aaaattttag tagacttttt agccattcaa ttttgggatt
    15901 cagttagtaa ctgattaact tagtaacaat tttcagattg taaatatacc ttatccaaga
    15961 gatatatgaa atgtaggttt atttcaacaa atcaatccaa ttaaaacttc tttttgctgt
    16021 tgaatcttcc cgtttgtaac caaactcctg ttactgctcg tttccacaat tatttagtat
    16081 gcctcaaaga ctcgaaggct caaagaaaga tttttatagt aatgttttaa ctttgagttt
    16141 tcgagtcttt aaacttagag tattggagtc tttgagtctt tgagtctttg aggtgattaa
    16201 aacgttttgg acgaataccc caacccagtt atttcttctt cttgcaattg tgtattatta
    16261 taaataattt tactataatt aaaacctata at
//
LOCUS       DAORVQ010000057        16241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1535_length_16241_cov_5.928951, whole genome shotgun sequence.
ACCESSION   DAORVQ010000057 DAORVQ010000000
VERSION     DAORVQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16241
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1535_length_16241_cov_5.928951"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1521
                     /locus_tag="PLX80_06425"
     CDS             40..1521
                     /locus_tag="PLX80_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY20468.1"
                     /translation="MAKVNSNDKKPNILIIWGDDIGQFNISVYNNGMMGYRTPNIDSI
                     GKEGAVFTDWYGQQSCTAGRAAFITGQSPIRTGLTKVGLPGAPEGMKKEDPTIATLLK
                     AQGYATGQFGKNHLGDSDETLPSNHGFDEFFGNLYHLNAEEEPEHTDYPKDPAFKKQF
                     GPRGVIHSYADGRIEDTGPLTKKRMETIDEEVNEKAIAFMEKTVKEDKPFFIWWNSTK
                     MHIFTHLKEGVVGKTGLGIYADGMVEHDRQVGEILDKLKELGVDDNTIVMYSTDNGAE
                     TCTWPDGGTTMFKGEKNTQWEGGYRVPALIKWPGTIKPGAVVNDICAHEDMLLSLVSA
                     AGNTTAKEDLLKGCEVNGTKYKVHLDGYDLGPALRGEEAWPRKEFIYWTDDGSVAALR
                     YGNWKVTFLEQPAEGLKVWIQPFTELRAPKITNLLMDPFERAEQEDAMGYQRWFIEHM
                     FMIAPAGAFVGKWLQSFKEFPPRQKPGSFNLDRVMESVMSSKG"
     gene            1795..3513
                     /locus_tag="PLX80_06430"
     CDS             1795..3513
                     /locus_tag="PLX80_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQY20469.1"
                     /translation="MKLKKLLLFSIITIIFASCNQSNNTATKDAADAIYFGGDIITME
                     GDNPAYAEAVAVKNGKIVFVGKKADAEKLKGDSTQMNDLKGKTLLPAFMDAHSHYINS
                     LFVANQCILYAPPSGPGKDVESIVDALQKFADERKIPKGEMIMGYGYDENVMPDGKLL
                     NRDDLDKAFPDNPVRVDHVSMHGCVLNSLAMKFYGISAETETPPGGVIVRKPGTDEPY
                     GLIMETAFLPIFEKSEPLTKEQEINWTKAGQMLYAESGITTAQEGATHLPQMETIKRA
                     SDAGVNIIDVVAFPFITDVDKILVKFPKSGWGKYNNGFKVGGVKITIDGSPQGKTAFF
                     SKPYLTGGPGGEESWYGELTFPQEMVNEMVKKVYEMNVPLLLHCNGDASIDAFLTAYE
                     NARSGDYSRPWNVTTIHSQFMRKDQMPKFVKYNVRPSFYTLHTYYFYEAHLNNRGMEE
                     AQYISPMHDAINMSMKPTNHTDFVVAPLDQMMMLWSAVNRISRAGALVGADQRVSPFE
                     GLQAMTINVANQYEEGDSKGSLKEGKRADLVILDKNPLKVELMMIKDIKVEETIKDGK
                     MIYKRK"
     gene            3652..3870
                     /locus_tag="PLX80_06435"
     CDS             3652..3870
                     /locus_tag="PLX80_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20470.1"
                     /translation="MNFLTERTPYEKISKNEIYKHNIALDWPKDNFALASIRDNGTKY
                     KVVEHPPVLKILKDCLKLLHGKEILTIE"
     gene            4367..4516
                     /locus_tag="PLX80_06440"
                     /pseudo
     CDS             4367..4516
                     /locus_tag="PLX80_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
     gene            4660..5697
                     /locus_tag="PLX80_06445"
     CDS             4660..5697
                     /locus_tag="PLX80_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQY20471.1"
                     /translation="MLLILFSIIICSCKDYTETVILKDRTSDSNSIPNDIYSPLFFEG
                     QLSHYVRKIYQDSRGNLWLGTNHYGVIRYNRDTLEYLSENQVIGGGRITGIIEDKEGN
                     VWFGTYTGLVRYDGKSFVNFSENDSQINKEIWSITLDKNGIFWIGTMEGVMKFDGEKF
                     TSFTLPESKIENENFILSPNRISSIMEDKKGNFWFGTDGAGIYKYDGELFTQFTMQEG
                     LCDNNVADILEDKDGNIWIGTMFGGVSRFDGKSFNNFTKSGAVSGEEVYGLYEDKKGN
                     IWFAAENFGVYRFDGNTFANFYTEDGLSSNGVQSIYEDKVGRFWFGGWKGLFRFDGEK
                     FFPVTKKGPWH"
     gene            5839..6261
                     /locus_tag="PLX80_06450"
     CDS             5839..6261
                     /locus_tag="PLX80_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HQY20472.1"
                     /translation="MNYSYKKNKLQEFGKKYTEAWCSQKLESVAEFFAVDGSLKVNNN
                     EPAAGRNAVTKVAESFMTAFPDMVISMDELITTDEDTEYHWTLTGTNTGLKGTGKSVR
                     VSGFELWQFNDEELIGNSIGTFDTDEYNRLLKFVVNNH"
     gene            6367..7086
                     /locus_tag="PLX80_06455"
                     /pseudo
     CDS             6367..7086
                     /locus_tag="PLX80_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
     gene            complement(7287..8975)
                     /locus_tag="PLX80_06460"
     CDS             complement(7287..8975)
                     /locus_tag="PLX80_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20473.1"
                     /translation="MKMFKTLIILLTLSNGSLTAQWQSDVRLTNNPAYSSTSENNSGC
                     IAASGNVVHVVWYDYREGNYEIYYKRSIDGGVSFQADTRLTNNTASSFSPSVSVSGLV
                     VHIVWNDRRDGNYEIYYKRSTDAGISWGADTRLTNNSAHSQFSSVAVSGSVVHVVWRE
                     MDDIYYKLSIDGGVSFGTDTRLTNNLAGSNGPSVSVSGSAVHVVWYDSRDGNFEIYYK
                     RSIDGGTSWGADTRLTNNSFLSTKVSVSVSGSVLNVTWVDERDSYKEIYYKRSTDGGT
                     SWEADTRLTIHTGTSSASDPSVSVSGSVVHVVWHDDRDGNTEIYYKSSSDGGVSFGAD
                     TRLTNNSAGSYYSFVSVSGSVVHVVWQEQRDGNEEIYYKRNPTGSQIPLTLNFTAFIE
                     GFYNPVTNSMISDTVRVYLKNVSFPFNNIDSSKGLLSSSGTGNFLFTNAMNGVNYYLA
                     VKHRNSIETWSSDPIIFVSGNSSYTFIALQSMAYGNNQIQIDSSPLRFGVYSGDVNQD
                     GAIDLTDAGMINNDVFLFASGYLNTDLNSDGVVDLDDGVFTDNNSYNFVTRITP"
     gene            9370..10827
                     /locus_tag="PLX80_06465"
     CDS             9370..10827
                     /locus_tag="PLX80_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20474.1"
                     /translation="MHNTALIDTLRTFSKQDLKEFGLFINSPFYNTNQSVIKMYNQIK
                     ILYPDFDEKYLDKKTLYNKSFGKMVYDDSFMRMTVFRLMELAKEFLVHVNLKRNNLLK
                     ETILLDELNNRELNKLLIKNIQELDRKIDKQKVKEADTYFVKYKMEYFRNDIKSRDTK
                     TITYKDKLNKDLLLEQKNLNIFFFLSSLKFFQYFLNQKNFVVNTEGYPDFVNSILEYL
                     KNNKDYMKVPAIKLYYIMTLLLLTEDDKYFFKLKKILFEDNDNLSYFEKFNLIANLRN
                     YAQKKFNEGQSEFKTSVIEIIKFSIKKNILTTAEGGKYVSELRFMTIVWVGIVSNELN
                     WIEKFIKEFINKIEPNKRQYVFAYNISILEFTKKNYTEALEILGKSGSIKNVFYKTAV
                     KQLTLMIYYELKWYTPAFDLLDSYKHFIKTDKLLPEIYKSKSSIFIKYYSKLLKIIES
                     TGDEKFVITKLISELKLTSQQWLLEKAEEFQKRSW"
     gene            11512..12489
                     /locus_tag="PLX80_06470"
     CDS             11512..12489
                     /locus_tag="PLX80_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY20475.1"
                     /translation="MTNKTDIIRSAALSGGGDKGAYTVGVLKKLYESGYRYDHIAGTS
                     TGALIAPLLATGQLGLLEHIYTSVEFKDILSKHDWALRILSEGAYYDVTPLRNLVRKH
                     ITNEVYDQLISPGTPDIYLAAVCMNNKALVYFTTSKNLKSSTKYDVIRIRDKSELIKG
                     IMASSVQPMIMPAEHHNGQAYADGGIKEFTPIDVLIDQSSDHITAIVTTPENSRYYQA
                     GNFKDPVDVAKKTIEILTEEIAINDLKGAETVNKGIKYSISIKERLERAGVANEIILS
                     ALTSEHDPFWGKRLIDFKIIRPLEWLSDGLTFDNKQMKRMFEIGYATKV"
     gene            complement(12867..13625)
                     /locus_tag="PLX80_06475"
     CDS             complement(12867..13625)
                     /locus_tag="PLX80_06475"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY20476.1"
                     /translation="MKRVENKVVIVTGGALGIGRETCILLAKEGAKVAVTDLLDKEGQ
                     ELADEITQSGGSASFWHLNVADEKEVEKVYSEVVKKFGKLDATVNNAGIAGADKPTHE
                     LTENEWDAVMNVNVKGVFFCTKHAIPLMKKSGSGSIVNLSSIYGIIGAGDIPTYHASK
                     GAVRLMSKNDALIYAKDNIRVNSVHPGFIWTPLVEKLGENVPGFRKDLDSLHPIGHVG
                     ESIDIAFGILYLVSDESKFVTGSELVIDGGYTCK"
     gene            complement(13922..14914)
                     /locus_tag="PLX80_06480"
     CDS             complement(13922..14914)
                     /locus_tag="PLX80_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HQY20477.1"
                     /translation="MDLSTIYMGMKLKNPLVASASPLSKTLDSAKNCEDAGLSAIVCH
                     SLFEEQLTHDAGELDHYLTTNAESYAEALTYFPEITEFNLGPSEYLEHINRLKNSLEI
                     PVIGSLNGVSNGGWINYAKQIEQAGADAIELNIFYIPTDFEKDSTVVEDMYVEIVTAI
                     KTVVKIPVAVKLSPYFSAMANMAKKIDNAGADALVLFNRFYQPDIDLETLDVFPNIEL
                     SNQWELRLPLRWIAVLHGNIKASLAATSGIHTHTDVLKVMMVGGDVAMVCSELLKNGI
                     HRATEILDDLKIWMEDKEYESIEQMQGSMDYRSVSQPAAFERANYMKVLNSYKI"
     gene            complement(14957..>16241)
                     /locus_tag="PLX80_06485"
     CDS             complement(14957..>16241)
                     /locus_tag="PLX80_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQY20478.1"
                     /translation="MEPQLPLKEQESKNWEHFLSIPEFDRTVVNTGLVKSSQLFQPLF
                     EFSGACTGCGETPYVKLVSQLYGDRAVIANATGCSSIYGGNLPTTPWTKNSDGRGPAW
                     SNSLFEDNAEFGLGMRISADKHNEYAGELLLRLRENIGDNLVNEILNADQSDEPGIYE
                     QRKRVEELNNKLKVMNTPDAEELLSIADYLVKRSVWIIGGDGWAYDIGFGGLDHVIAS
                     GKNVNILVLDTEVYSNTGGQMSKATGLGAVAKFATSGKLTPKKDLAMMAINYGSVYVA
                     KVAMGANDTHTLRAFLEAESYDGPSLIIAYSHCIAHGINMEKAIDHQKNAVKSGYWPL
                     FRYDPRLIDENKNPFRLDSGEPVIKLEEYAYAQARYKMLTKSDPLQAKKLMKLAQEDV
                     KKRFDIYKRLAESKPNSEPETKSNSESKESNSITN"
BASE COUNT         5472 a         2712 c         2998 g         5059 t
ORIGIN      
        1 aaacaaataa ttttatttca aaaacataaa caaaataaaa tggctaaagt aaatagtaat
       61 gacaaaaaac ctaatatcct tattatctgg ggagatgaca tcggacaatt taatatcagc
      121 gtttacaaca atggaatgat ggggtacaga actcctaaca ttgacagcat cggaaaagaa
      181 ggcgctgtat ttacagactg gtatggtcag caaagctgca ctgcaggaag agcggctttt
      241 attacaggtc aatcaccaat aagaacaggt cttactaaag taggacttcc cggagctccg
      301 gaaggcatga agaaagaaga ccctacaatt gctacattat taaaagctca gggctacgca
      361 accggtcagt ttggtaagaa tcatcttggt gacagtgacg aaacgctccc gagtaatcat
      421 ggctttgatg aattttttgg taatctttat caccttaatg cggaagaaga acctgagcat
      481 actgattatc ccaaagatcc cgcattcaaa aaacaattcg gtccccgcgg tgtgattcat
      541 agttatgccg acggacgaat tgaagatacc ggtcctctta caaaaaaaag aatggaaaca
      601 attgatgaag aggtaaatga aaaagcgatt gcttttatgg agaaaactgt taaagaagac
      661 aaaccattct ttatctggtg gaactctacc aagatgcata tcttcactca cctgaaagaa
      721 ggagttgtag gaaaaacagg gctgggaata tatgctgatg gaatggtgga acatgacaga
      781 caggtcggcg agattctgga taaattaaag gaattgggcg ttgatgataa tacaattgta
      841 atgtattcaa ctgataacgg cgctgaaacc tgtacctggc ctgacggcgg aactacaatg
      901 ttcaaaggtg aaaaaaatac acaatgggaa ggcggctatc gtgtgcctgc acttattaag
      961 tggcccggca caataaaacc aggtgcagtt gtaaatgata tctgcgctca cgaagatatg
     1021 cttctcagtt tagtttcagc cgcaggaaac acaactgcga aagaagattt actaaaaggt
     1081 tgtgaagtta atggaacaaa gtataaagtc catctcgacg gatatgatct cggtccggca
     1141 ttaagaggag aggaagcctg gccacgcaaa gagtttatct actggactga tgacggcagt
     1201 gtagctgcat taagatacgg caactggaaa gtaacatttc tggaacaacc tgctgaggga
     1261 ttgaaagtat ggattcaacc gtttacagaa ctccgtgctc ctaaaattac aaaccttctt
     1321 atggatccat ttgagcgtgc tgaacaagag gatgcaatgg gttatcagcg ctggtttatt
     1381 gaacatatgt ttatgatcgc tcctgccggc gcttttgtcg gaaagtggct tcagagcttt
     1441 aaggaattcc cgccgcgtca gaaaccggga agttttaacc tggacagagt gatggaaagt
     1501 gtaatgagct cgaaggggta attgatttgg gatttcggat ttggaatttt tagatttcag
     1561 atgttagatt ttagatttga atattaagcc gtcatgtagt ttttcgtgac ggctttagtt
     1621 taatcatatt gaatttgcag atgttaaaaa ttaaattgat catcttcttt aacaaaggaa
     1681 acctatttga acaacttttg cagtcagttg tcacagtgaa aagattgccg gaggtaaagt
     1741 tttgagaatt aaaacgtgag caccacatct atattttatt ttaattaaaa aaacatgaaa
     1801 ctaaaaaagc ttttactctt cagtattatt acaataattt ttgcaagctg caatcaatca
     1861 aataatactg caactaaaga tgcagcagat gcgatctatt tcggcggtga cattataaca
     1921 atggaaggcg acaacccagc atatgctgaa gcagttgcag tgaagaacgg caagattgtt
     1981 tttgtcggaa agaaagccga tgctgaaaaa cttaaaggcg acagcactca aatgaatgat
     2041 ctgaaaggaa aaactttgct tccggcattt atggatgcac atagtcatta tataaattca
     2101 ttgtttgttg ctaatcagtg tatattatat gcgcctcctt ccggacccgg taaagatgtg
     2161 gaaagtattg ttgacgcttt gcagaaattt gcagatgaac ggaaaattcc aaaaggtgaa
     2221 atgatcatgg gttacggata tgatgagaat gttatgcctg atggaaaatt actgaaccgt
     2281 gatgatcttg acaaagcatt tcctgataat cctgtaagag ttgaccatgt ttcaatgcat
     2341 ggatgtgttc ttaacagtct tgctatgaaa ttttacggga tcagtgcaga aactgaaact
     2401 cctcccggtg gtgtgatcgt tcgtaaaccc ggaacagatg aaccgtacgg tctgattatg
     2461 gaaactgcct tccttcctat ttttgaaaaa tccgaaccat tgacaaaaga acaggaaatt
     2521 aactggacaa aagcaggtca gatgctttat gccgaatcag gaattacaac tgcgcaggaa
     2581 ggtgcaaccc atcttccgca aatggaaact attaagagag catctgatgc aggagtaaat
     2641 attatagatg ttgttgcttt tccattcata actgatgttg ataaaatact tgttaagttt
     2701 ccaaaatcag gatggggtaa atacaataat ggtttcaaag ttggcggagt gaaaattact
     2761 atagatggtt ctcctcaggg gaaaacagca ttcttttcaa agccatattt aacaggcgga
     2821 ccgggcggag aggaaagctg gtacggcgaa cttacatttc cacaggaaat ggtgaatgaa
     2881 atggtaaaaa aagtttatga aatgaatgtt ccactgttac ttcattgtaa cggagatgct
     2941 tcgatcgatg ctttcctgac agcttatgaa aatgcaagat caggtgatta cagcagaccc
     3001 tggaatgtga caactatcca ttctcagttt atgcgaaaag atcagatgcc gaagtttgta
     3061 aagtataatg ttcgtccgtc tttttacaca ttgcatacat attatttcta tgaagctcat
     3121 ttaaataacc gcggaatgga agaagctcaa tatataagtc cgatgcatga tgctattaac
     3181 atgagcatga aaccgacaaa tcatacggat tttgtagtag ctccattaga tcagatgatg
     3241 atgctctggt ctgccgtgaa ccgtatttcc agagccggtg cattagtcgg agctgatcag
     3301 cgcgtatctc cgtttgaagg tttacaggca atgactatta acgttgcaaa tcaatatgaa
     3361 gaaggtgatt caaaaggaag tctgaaagag ggaaaacgtg ctgatctggt gatactggac
     3421 aagaatccgc tgaaagttga actgatgatg atcaaagata ttaaagttga agagacaatt
     3481 aaagacggta aaatgattta taaaaggaaa taatttataa tttaaaacca tctttctaat
     3541 caggagagac tatcctaaaa caaaatccgc cgtcggtaaa ttaacaatgg gctctctttc
     3601 aatttgagtt agtacgaatt attttcgtag taatacgatt ttttgtagaa aatgaacttc
     3661 ttaactgaaa ggacccctta cgaaaaaatt tcaaaaaatg aaatttataa acacaacatt
     3721 gctttagact ggccaaagga taattttgca ctggcatcga tccgtgacaa cggtactaag
     3781 tacaaagtag ttgaacatcc tccggtctta aagattttga aagattgtct aaagctgctt
     3841 cacggaaaag agattttaac aattgaataa actactgcag cgcaacgtct ttatgttaaa
     3901 cttaaagatt atgtggatat aatattgata tgcgaaccct ttcacaacag ttaaatgtaa
     3961 ttttccaaat ttttttatcc atttctgtat agtttgacct ccgccgatac cgtttaattt
     4021 tcttgctccc tcttgagata acttcccgct ctctgtttaa acttggacgt acataaaact
     4081 ttttgttttc cagcaacaaa gctacttaac gttcgtgctg ttgatccatg ctagttaaca
     4141 gctcgaaagt tttccctcaa tgaaatatta aactgttcag aagtattagt aaatttttga
     4201 gaacatatac atgggcttaa aatactttct gtcagattaa aggaagagaa tataaatttt
     4261 attgcaagaa aataggaagt atattataag ttttaaaatt agaatttttt atattttaca
     4321 aaaaaagtaa acttaagata tggagcagtt taaaaatgac aattaaacgt caagctggcc
     4381 gaagaaacca tccgagatca gaaaactcgg cggtgctata tttggtgact ttcgtttcga
     4441 tactgtcttc gtttatcaca acagcgcgga ttcttattat ggaggcaggg gatttcgcgg
     4501 atcacttaga gtctgatttt aaaaatgaag cagaaatttt aaaaagttaa taatatttta
     4561 gagtcagtct gaaactgaaa tgaaaatcaa aacagatttg aatattaaaa taagaatata
     4621 ttgattgtca taaaaaggta caaaaagttc agatcaactt tgctcttgat tttattttct
     4681 ataattatct gctcttgtaa agattacacc gaaacagtta tactaaaaga cagaacttct
     4741 gattcaaatt ctattccaaa tgatatttac tcccctttgt tttttgaagg acaattaagc
     4801 cattatgtaa ggaagatcta tcaggatagc agaggtaatc tttggctagg cactaatcac
     4861 tatggtgtca tccgttacaa tagagataca ctggaatatt tatctgagaa ccaggttatt
     4921 ggtggcggtc ggataacagg aattatagaa gataaggaag gtaatgtttg gtttggaacg
     4981 tataccggat tggtcagata cgacggaaaa tcatttgtaa atttcagtga gaatgacagt
     5041 cagataaaca aagaaatctg gagtataaca ttagataaaa atggaatatt ctggatcggt
     5101 accatggaag gggtaatgaa atttgatggt gaaaagttta catctttcac tcttccggaa
     5161 tctaaaattg aaaacgaaaa tttcatctta tctcccaatc ggatttcgag tattatggaa
     5221 gataaaaaag gaaacttttg gtttggtact gatggtgcag gtatttataa atacgatgga
     5281 gaattgttta cacaatttac tatgcaggag ggactttgcg ataataatgt tgcagatatt
     5341 ctggaagata aagatggtaa tatttggatc ggtactatgt ttggaggagt aagtcgtttt
     5401 gacggaaagt catttaataa ttttacaaaa agcggtgcag tcagcggtga agaagtatat
     5461 ggcttatatg aagacaaaaa gggtaatata tggttcgcag ctgaaaactt tggtgtgtac
     5521 cgatttgatg gaaatacatt cgcaaatttt tacactgagg acggtctcag ttcaaatggg
     5581 gtacagtcta tttatgaaga taaagtagga aggttctggt ttggcggctg gaaaggactc
     5641 ttccgttttg atggagaaaa atttttccct gtcaccaaaa aaggtccctg gcattgatct
     5701 taattaaatt agatgctttc cggaatagaa atgtttgaga cggatttaat ataattggaa
     5761 atactcagag ttaagagagc acgagatttt ggaaatgaaa gagtttattt aatttcacta
     5821 atcaaaaaat attgaataat aaattacagt tataaaaaaa ataaattaca ggaattcgga
     5881 aagaaataca cagaggcatg gtgcagtcag aaactggaaa gtgttgctga attttttgca
     5941 gtagacggtt cattgaaagt aaacaataat gaacctgcag ccggaagaaa tgccgtcaca
     6001 aaggttgcgg aaagttttat gacggctttc ccggatatgg ttatttcaat ggatgaactt
     6061 ataacaactg acgaagatac agaatatcac tggacactta ccggtactaa taccggacta
     6121 aaaggcacag gaaaaagtgt tcgcgtcagt gggttcgaac tttggcagtt taatgatgag
     6181 gaactgattg gaaattccat aggaactttt gatactgatg aatataaccg actgctaaaa
     6241 tttgttgtaa ataatcatta gaaattttta taattcttaa tttttttata gatatgaata
     6301 atttcacaga aatgttcaac ataaaatatc cgatagttca ggcacctaca gacattagaa
     6361 tcagggttaa aaataattca attttcctgg ggaatgccaa atgatgaaat gttagaattg
     6421 atcaaattct ataaagcaaa aataggaatt caggtaactg acacagaaag cgcggttaag
     6481 gcagtttcac ttggagcgga ttttttagta tgtcagggga cacaggcagg cgggcattta
     6541 caggcttcaa agtcacttga aaaagcttta aaggaagttc ttgaagttgc agaaaatgat
     6601 ataccagtac ttgcttccgg aggtatatca tccggaaagg atatgaagca ttatttggat
     6661 ttaggagcat ccggtgtagt aatgggatca cgttttatag cttcagaaga aagcattgcg
     6721 cattctgtat ataaagagtc acttgtgagt tctaagagtg atgatacagt attaacagtt
     6781 tgcatgaata aaggctggga taattctata tcgagaataa ttcgaaactc aaccttcagg
     6841 atgtgggagt ctgcaggttg tccggttata agtaacagac ccggtgaatt tgatgtaatc
     6901 ggtaaaaatc tgaaaggtga tgatattgag cgatatagta tcagcgcacc tgcagcagga
     6961 gttacaggtg acatagaagc tatgactttt tatgcaggta aaagtttaaa taatattaac
     7021 ggagtcaaat ctgtaaagaa tattattgaa gatgtatgga atgaatattt aagctgtaat
     7081 aaataatatg aaaaatcaag aattttaaat tattgtataa gaatttatca gaattttaga
     7141 aatataaaat tatgaacggt gaaattcatc acatagaaaa atatgcctcg gatcttaaca
     7201 gatcaattat tttctgggta tgtttctgac cgagaaattt aattatacaa aagaaaaatt
     7261 ttaaaaatat aaattgatat atattctcaa ggagtaattc gggttacgaa attgtatgaa
     7321 ttattatcag taaaaactcc atcatctaaa tccactacgc cgtcactgtt aagatctgta
     7381 ttcagatatc cactggcaaa aaggaacaca tcattattaa tcataccggc atcagtaaga
     7441 tctattgctc cgtcctgatt tacgtcgccg ctatagactc caaatctcaa aggagatgaa
     7501 tctatctgta tctgattatt cccatacgcc atagattgca aagcaataaa tgtataggat
     7561 gagtttcccg aaacaaaaat tataggatca ctgctccagg tttcaattga gtttctgtgc
     7621 tttacggcta aataataatt tacaccgttc attgcatttg taaaaagaaa atttcccgta
     7681 cctgaagaac ttaataatcc ttttgaagaa tcaatattat taaatgggaa agatacattt
     7741 tttaagtata ctctgacagt atcgctaatc atactatttg taacaggatt ataaaatcct
     7801 tcaataaatg cagtgaaatt taaggtcaga ggaatttgtg aaccggtcgg attgcgttta
     7861 taatatatct cctcgttccc atcacgctgt tcctgccata cgacatgaac aaccgagccg
     7921 gatactgata cgaaagaata gtatgaccct gcagagttat tcgtcaaccg tgtatctgct
     7981 ccaaagctaa ctcctccatc tgatgagctc ttataatata tttcagtgtt cccgtcacga
     8041 tcatcatgcc atacaacatg cacaacagaa ccggatactg atacggaagg atcggatgca
     8101 gatgaagtac cagtgtgaat tgttaatctt gtatctgctt cccagcttgt gcctccatca
     8161 gtcgagcgtt tgtaatatat ctctttgtac gaatcacgtt cgtccaccca tgtaacattc
     8221 aaaaccgagc cggatactga aacagaaact ttggttgaaa gaaaagagtt attggtcagc
     8281 cgcgtatcag ctccccagct tgtacctcca tctatcgagc gtttgtaata tatctcaaag
     8341 tttccatcac gagagtcata ccatacaaca tgcacagccg agccggacac tgatacggaa
     8401 ggtccatttg aaccggctaa gttgttcgtc agccgtgtat ctgttccaaa gcttacgcct
     8461 ccgtctattg agagtttgta gtaaatatca tccatctcac gccacacaac atgcacaacc
     8521 gagccggata ctgcaacaga agaaaattgt gaatgagcag agttattcgt cagacgcgta
     8581 tctgctcccc agcttatgcc ggcatctgtt gagcgtttgt agtatatctc atagtttcca
     8641 tcacgtctgt cattccatac gatatggaca accagaccgg atactgatac ggaaggagaa
     8701 aatgaagaag cagtgttatt cgtcagtcgc gtatctgctt gaaaacttac acctccgtct
     8761 atagagcgtt tgtaatatat ctcatagttc ccttcacgat aatcatacca tacaacatgt
     8821 acaacatttc cgcttgctgc aatgcaccct gaattattct cagatgtact ggaataagca
     8881 ggattatttg tcagacgcac gtcagactgc cactgagcag ttaagctgcc attacttagg
     8941 gtaagaagaa taattaaggt tttgaacatt ttcatatata taatttttag ttttataatt
     9001 tttttaaatt ttattttagc ataaaatgat taatcgcttc aaacattgct cgattcagat
     9061 ttataagata tttcaatttt taggtctgaa attgcaattt caataatata agaaacagct
     9121 ttctgaacag attcttattg caggataatc tctaattttg aatctctttt gccgaagaca
     9181 aattattcat tattgtcaat ttaaatttac tcgttcaaaa atagtgatgc ttaaatgata
     9241 attcaaagaa agttattttg aattgttttc gggtttatat tggcaacatt tctaatttag
     9301 ttgtttttta aagaatttta gacaatttta tgatttcacc gtaaccataa atttataata
     9361 tatttaagaa tgcataacac ggctttaatt gatacattaa gaacatttag taaacaggat
     9421 cttaaagaat ttggattgtt catcaattca ccattttaca acacaaatca aagtgttata
     9481 aaaatgtata atcagatcaa aattctttat cctgatttcg atgaaaagta tctggataaa
     9541 aaaacattat acaataagtc attcggaaag atggtatatg atgacagttt tatgagaatg
     9601 acagtgtttc gtttgatgga gttagcaaag gaatttcttg tccatgtgaa tttgaaacgt
     9661 aacaatttgc tcaaagaaac cattctgctg gatgaactta ataaccgcga attaaataaa
     9721 ctgctgatta aaaacataca agagcttgat agaaaaattg ataagcaaaa agtaaaagag
     9781 gcagatacat attttgttaa gtataaaatg gagtatttca ggaatgatat taaatcaaga
     9841 gatacaaaaa caattaccta caaagataaa ctgaataagg atcttttgct tgagcagaaa
     9901 aacctaaata tatttttctt tttaagctca ctaaaatttt ttcagtattt tttgaatcaa
     9961 aaaaattttg ttgttaatac agaagggtat cctgattttg ttaacagtat tctggaatat
    10021 ctaaaaaaca ataaagacta tatgaaagta cctgcaataa agttatacta cattatgaca
    10081 ttactgctgc tgactgaaga tgataaatat ttttttaagc ttaaaaaaat attatttgaa
    10141 gacaatgata atttgagtta tttcgaaaag tttaatttga ttgcaaattt acgaaactat
    10201 gctcagaaaa aatttaatga aggacaaagt gaatttaaaa catctgtaat tgaaataata
    10261 aagttttcta taaaaaagaa tattcttacc accgcagagg gagggaagta tgttagtgaa
    10321 ttgagattta tgacaatagt atgggtcgga attgtgtcta atgaattaaa ctggattgaa
    10381 aaatttatta aagaatttat caataaaata gaacccaata aaagacaata tgtatttgca
    10441 tacaatatat ctattcttga attcacaaag aaaaattata cagaagcttt ggaaattctt
    10501 ggtaaaagcg gatctatcaa gaatgttttt tataaaactg cagttaaaca attaacactg
    10561 atgatatatt acgaacttaa atggtatact cctgcatttg atctgcttga ttcatataaa
    10621 cattttatta aaactgataa acttcttcct gaaatatata aatctaagtc cagtatattc
    10681 attaaatatt attcgaaatt attgaaaatc attgaaagta caggtgatga aaaatttgtt
    10741 ataacaaaac tcatctcaga attgaaatta acttctcagc aatggctttt ggaaaaagct
    10801 gaagagtttc aaaaaagaag ctggtaattt ttaaaatccg gaatcaataa tttacccgtg
    10861 ctttaagtga taaatattat ctgctttttt aatttaaaga ctttaacaaa ttcacctgtt
    10921 caaacaagat agaaaatgtg attagatttt ttctttcgat aaaaatattt tctgtttttt
    10981 aaattgtgaa tcattgaaag ggaaatatcc gaagatttta ttttcctgaa aaaagtataa
    11041 atagtctaat aatagactaa tacaggttcc gttacatcac atagaaatat gtgtttcgga
    11101 tcataaaaag accaattaat atcagggtat ggtttctaac cgaaaaattt agttatacag
    11161 tttttcaaaa atgggattcc ggaataagat ttacatttaa tgaaacatac attgtattgg
    11221 ctcatactga aaaaaaatat ctggacaatt cctttaacag aaaaaacaca ggattgaatc
    11281 atcttacttt tactgtgcat cgcgagagtt tgtagaaact ctgacggaaa agctaaaaga
    11341 aatgaatata tacattcttt attcagacag acattatttt gtccctgtca gattaaagga
    11401 agtgaatata aattttattg caagaaaata ggaaatatat tgtaagtttt aaaattagaa
    11461 ttttttatat tttacaaaaa aagtaaactt aagatatgga gcagtttaaa aatgacaaat
    11521 aaaacagata taatcagatc agcagcatta agcggcggtg gtgacaaagg tgcttataca
    11581 gtaggtgtat taaagaaatt atatgaatca ggttatagat atgatcatat tgccgggact
    11641 tcaaccggtg cattaattgc gcctctgctg gcaacaggtc aattaggact tcttgaacat
    11701 atttatacat cggttgaatt taaagatatt ttaagtaagc atgactgggc attaagaata
    11761 ctttccgaag gtgcttatta tgatgtaact ccactaagaa accttgtcag aaaacatatt
    11821 acaaatgaag tttatgatca acttataagc ccaggcactc ctgatattta tctggcggca
    11881 gtttgtatga ataataaagc tctggtatat tttactacat cgaaaaattt gaagagctct
    11941 acgaagtacg atgtaataag aataagagat aagtcagaac taataaaagg tataatggca
    12001 agttctgttc aaccaatgat aatgccggct gaacatcata acggtcaagc ttatgctgac
    12061 ggtggaatta aagaattcac tcccattgat gtattaatag atcagagctc tgaccacata
    12121 acagcaattg ttacaactcc cgaaaatagc agatattatc aagccggtaa ctttaaagat
    12181 cctgttgatg tagcgaaaaa aactattgaa atactgacag aggaaattgc aatcaacgat
    12241 ttgaaaggtg ctgagacagt taacaaagga attaaatata gtatcagcat taaagaaaga
    12301 cttgaaagag ccggagtggc taacgaaata attctttcgg cacttacgtc tgaacatgac
    12361 ccgttctggg ggaaaagact tatagacttc aagattatcc gacctttgga atggttaagt
    12421 gatggattaa cattcgataa taaacaaatg aaaagaatgt ttgaaattgg atatgcaact
    12481 aaagtataaa taccaaatag cagttgcata tatttgagcc tatattagca tctcattaag
    12541 tttgagtcat tgttggagtt ccaataagat tgatgaattc agactgcagt ataaatttga
    12601 aattacataa aacttttttg gtctctaaaa acatagccat tcaccgtttg tactattgat
    12661 ctcagcttgt tggtggcttg aaagtttgcg tcattgattt attaaactgt tcagtacatt
    12721 atgcaaactt taaagaacgc ctataagggc attaatcccc gtattagtca aaagcttaaa
    12781 taagatacaa ttttaattta aagtttaatc aaacatactt ttagaaaacc aacaacggtt
    12841 caatgctatt gtaaatttaa gactttctat ttacacgtat agccaccatc aataaccaat
    12901 tcacttccgg taacaaattt tgattcatct gacacaagat aaagtattcc aaaagctata
    12961 tctattgatt caccaacatg tcctatcgga tgaaggctat caagatcttt tcggaatccc
    13021 ggaacatttt cacctaattt ttcaaccaac ggtgtccaga taaagccagg atgtacagaa
    13081 ttcactctta tattatcttt tgcataaatt aatgcatcat tttttgacat taatctgact
    13141 gcaccttttg aagcatggta tgttggaata tctccggcac cgattattcc atatattgaa
    13201 gaaagattta caatgcttcc gcttccggat tttttcatga ggggaattgc atgtttcgta
    13261 caaaagaata ctccttttac attcacattc ataactgcat cccattcatt ttctgttaac
    13321 tcatgggttg gtttatcggc tcctgctatt cctgcattat ttacagttgc atcaagttta
    13381 ccaaattttt tgactacttc tgaataaact ttttcaacct ctttttcatc tgctacattc
    13441 agatgccaga aagaagctga tccgcctgac tgtgtaattt catcagctaa ctcctgacct
    13501 tctttatcaa gtaaatctgt tactgcaact ttcgcacctt cttttgcaag caaaatgcaa
    13561 gtttcccgtc ctatacctaa tgcaccaccg gttacgataa caactttgtt ttcaactctt
    13621 ttcatgattt taatattttt ttagttaatt aaatttatgt tttatatttg tataaaaata
    13681 tccaacagtt tggaaattac cactattgct ttcaggtcaa ttttgtttaa ttttagtttg
    13741 ccaattccaa caataaattt acttgccggt cgtaatattg atctcagctt gttggatgtc
    13801 caatagaaca ctctgtcatg acagatcgga ttctgagatt gctactttgc ttaatacttc
    13861 aaaagtcatt tccgccaagt gattaaaatt ttatatttgt tatttaaaat ttattattgt
    13921 ttcaaatctt ataactattc aacaccttca tatagtttgc tctttcaaaa gctgccggct
    13981 ggctcacaga tctgtaatcc atgctgccct gcatctgctc aatggattca tattctttat
    14041 cttccatcca tatcttcaga tcatcgagaa tttcagttgc tctgtgaata ccattcttta
    14101 ataattcaga acagaccatt gcaacatcac ctccgaccat catgactttc aaaacatctg
    14161 tgtgagtgtg aataccgctc gttgctgcaa gggaagcttt gatatttcca tgaagaacag
    14221 ctatccatct cagaggcaat cttaactccc attgattact cagctcaata ttcggaaata
    14281 catcaagagt ttccagatcg atatccggct gataaaatct gttgaaaagt accaatgcat
    14341 cagcacctgc attatctatt ttctttgcca tgtttgccat tgcagagaaa taaggactga
    14401 gctttacggc cacaggaatt tttactactg tctttatagc tgtaacaatt tcgacataca
    14461 tatcttccac aactgtacta tccttttcaa aatctgtcgg aatgtaaaaa atatttaatt
    14521 ctatcgcatc cgcacctgcc tgttcgatct gctttgcata atttatccag ccgccgttac
    14581 tgactccgtt cagacttccg atcacaggaa tttccagaga gttttttaat ctgtttatat
    14641 gctcaagata ttcagaaggt ccaagattga attcagtgat ctcgggaaaa tatgtaagtg
    14701 cttcagcata actttcagca ttggtagtaa ggtaatgatc aagttcaccc gcatcatgtg
    14761 taagctgctc ttcaaataaa gagtggcaga ctattgcaga cagtccggca tcttcacaat
    14821 tctttgcact gtcaagtgtt ttggacagcg gagatgctga tgcgactagc gggtttttta
    14881 atttcatgcc catgtatatt gttgaaagat ccattttata agaattaaaa attaaaaatt
    14941 aaaaattaaa attattttaa ttagttattg aattactttc ttttgattca gaatttgatt
    15001 ttgtttcagg ttctgaattt ggttttgatt ctgcaagtct tttgtaaata tcaaatctct
    15061 tttttacatc ttcctgtgca agtttcatta atttctttgc ctgcaaggga tcagactttg
    15121 taagcatttt atatcgtgcc tgtgcataag catattcttc aagcttgata acaggttcac
    15181 cggaatccag tctgaatgga tttttatttt catcaataag acgcggatca tatctgaaca
    15241 gcggccagta accgcttttg acagcattct tctgatgatc aatagctttt tccatattta
    15301 ttccatgtgc tatacaatgt gaatatgcaa ttatcagaga aggtccgtcg taagattctg
    15361 cttccagaaa tgctcttaaa gtgtgagtat catttgcacc cattgcaacc tttgcaacat
    15421 agacgcttcc gtaatttatt gccatcattg caagatcttt tttcggagtc agcttgccgg
    15481 atgtagcaaa ctttgcaact gctccaagcc ctgtagcttt tgacatctgt ccaccggtgt
    15541 ttgagtaaac ttcagtatca agtacaagta tatttacatt ttttcccgat gcaataacat
    15601 ggtccagtcc gccaaatcca atatcatatg cccagccgtc accgccgatt atccagacag
    15661 atctttttac aagatagtct gcaattgaca gaagttcttc agcatcagga gtattcatca
    15721 ctttcaattt attattcaat tcttcaactc tttttctctg ttcataaata cccggctcgt
    15781 ctgattgatc tgcatttaaa atttcgttaa ctaaattatc accaatattt tctctaagtc
    15841 ttaaaagtaa ttccccggcg tattcattat gtttatcagc agagattctc atacccagac
    15901 caaactctgc attatcctca aaaagagaat ttgaccatgc aggtcctctg ccatcactat
    15961 tcttagtcca tggtgtagtt ggaagattac ctccatagat cgatgaacag ccggtagcat
    16021 ttgcaatcac tgctctgtct ccataaagct gtgaaacgag tttaacgtaa ggtgtctcac
    16081 cgcagccggt acatgcgccg gagaattcaa acagcggttg aaagagctga ctgcttttta
    16141 caagtcctgt gttgacaaca gttctgtcaa attccggaat tgacagaaag tgttcccagt
    16201 ttttggattc ctgttctttt aatggaagct gcggctccat g
//
LOCUS       DAORVQ010000058        16145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1554_length_16145_cov_5.935426, whole genome shotgun sequence.
ACCESSION   DAORVQ010000058 DAORVQ010000000
VERSION     DAORVQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16145
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1554_length_16145_cov_5.935426"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..598
                     /locus_tag="PLX80_06490"
                     /pseudo
     CDS             44..598
                     /locus_tag="PLX80_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679894.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            620..1708
                     /gene="nuoH"
                     /locus_tag="PLX80_06495"
     CDS             620..1708
                     /gene="nuoH"
                     /locus_tag="PLX80_06495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQY20479.1"
                     /translation="MNFSIYDFTGFVSSVDLWLRSNFPPLVADLTGFVIIAICFLTFV
                     TLLGLSLVYIERKVCAFFQQRLGPMRVGFWGTAQTIADIIKLIFKEPLITKNSDKFLF
                     NLAAFIVIVAPIMAIAAVPFAKGLHVLDFDIGLFFITAISSIGVTGILLAGWSSNNKY
                     SLIGAMRSGAQIVSYELSVVLSLLTIIVLTGSLQLSVIIESQRDGWWIFKGNIPAVIA
                     FFIFIIASTAESNRGPFDMAEAESELTAGFHTEYSGIKFAFFFLAEYINLFIIAAVGA
                     TVFFGGWMPLHIGNFEFFNNVMDFIPPIVWFFGKISMLIFLIMWFKWTFPRLRIDQLL
                     TLEWKYLLPINLINLLLMSLVALNGWHF"
     gene            1811..2230
                     /locus_tag="PLX80_06500"
     CDS             1811..2230
                     /locus_tag="PLX80_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQY20480.1"
                     /translation="MKVTGHYFIHPKEIITQQYPENKDTLKMFDRFRGEVVLIHDENN
                     LHKCTGCSACELACPNGSIEIISDKEIDPETGKSTKIIDTFVYHLSMCTFCNLCILSC
                     PTDAIKMAQNFEHSVFDRSELTKVLNKPGSKIMPGVK"
     gene            2246..2755
                     /locus_tag="PLX80_06505"
     CDS             2246..2755
                     /locus_tag="PLX80_06505"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQY20481.1"
                     /translation="MSETVFYILASFILIFSVLTVTSRKILRTAVYLLFVLLGTCGFY
                     FAMEFNFLAAVQLTVYAGGIVVIIIFSIMLTSDLQDKLPAVKFKKIFFSLIAVLFGAA
                     VCITTILSYEFPAMTTKVLDSSMRTIGLRLLGYQENGFVLPFEVISILLLAAMIGSII
                     IAKKADTEN"
     gene            2774..3091
                     /gene="nuoK"
                     /locus_tag="PLX80_06510"
     CDS             2774..3091
                     /gene="nuoK"
                     /locus_tag="PLX80_06510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQY20482.1"
                     /translation="MTEVVGIQHFMVLSTLMFFIGVYGFLTRKNLITILLSIELILNS
                     VNINFVAFNKYLYPENLGGHFFAMFVIAVAATEVAVAIAIIISLYRKLKMIDVENVNE
                     MKY"
     gene            3115..5037
                     /gene="nuoL"
                     /locus_tag="PLX80_06515"
     CDS             3115..5037
                     /gene="nuoL"
                     /locus_tag="PLX80_06515"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQY20483.1"
                     /translation="MENFYYTLIIPVILFFTFVFTGLLGRKFKPLFSGMVCTASIFIS
                     TALSWYTAYQYFFVQGKVDGAYKLINVFNLEWLRFNETLSIDIGILLDPISVMMLVVI
                     TTVSLMVHLYSLGYMKGDPGFSRYYAFLSLFTFSMLGLVLSVNIFMTYMFWELVGVSS
                     YLLIGHYYDKPSAVSASKKAFIVTRFADLGFLIGILLLSAYAGSFDFNELTNPQGSAI
                     LQSASVTFMGLSAVTWALILIFIGCAGKSAMFPLHIWLPDAMEGPTPVSALIHAATMV
                     VAGVYLIARLFPLYIFTSPQALEVVAYVGAFTSLFAAVIACTQTDIKRVLAFSTISQI
                     GYMMVALGVSGYGGEEGLGYMASMFHLFTHAMFKALLFLGAGSIIHAIHTNEICDMGN
                     LRKYMPVTHITFLIACLAIAGIPPLSGFFSKDEILVAVIEKNFFLFIVQYIVAGLTAF
                     YMFRLYFIVFWNKEKKYEHEPHESPSIMLITLVFLAVCSAVAGFIPFSEFVTSDGLPF
                     VTHIHMKVAVPTVTLAVIGILTAFFLYKKESTSPDKIKISLGLIYRSAYRKFYIDEVY
                     MFVTKKIIFNMISVPIAWFDRNVIDSSMNGIAAATNFISEKIKYLQSGQLQQYAFVMV
                     AGMILIFLLTVYLITL"
     gene            5045..6544
                     /locus_tag="PLX80_06520"
     CDS             5045..6544
                     /locus_tag="PLX80_06520"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQY20484.1"
                     /translation="MDLIFLILIPVLTVFAIILTDSKAQARIAAVAGTGIQLMLSIYL
                     VILYLMERSSGNIEQMLFSNKYVWFTSLNIYFSTGVDGISVSLILLTGIITFTGVLAS
                     WDVDYLYKEFFISLIVLATGVFGFFISLDLFTMFLFYELAVIPMYLLIGIWGSGKKEY
                     SAMKLTLMLMGASAILLVGLLGIYSYSSPTGLTPLTFNIIEIAQIDIPIEAQRYFFPM
                     IFIGFGVLGALFPFHTWSPDGHASAPTAVSMLHAGVLMKMGGYGCLRVAMYLLPEAAN
                     EMAWIFIILTSISIVYGAFGAIVQKDLKYINAYSSVSHCGLVLFAVLMSNEIAMNGAV
                     LQMISHGLMTALFFALIGMIYGRTHTRDIRNMSGLMKMMPFLAVCYMIAGFASLGLPG
                     LSGFVAEMTIFVGAFQNADLFHRGVTVLACSSIVITAVYILRVVAKILLGPADKSMPA
                     GMTDAVWNEKAVTVILIIAIAGMGLAPLWLSDMILNSLEPVVQKLTLLK"
     gene            6566..7990
                     /locus_tag="PLX80_06525"
     CDS             6566..7990
                     /locus_tag="PLX80_06525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQY20485.1"
                     /translation="MTFEQLLLMRHELLLIIVAVIILIIELSTDNKNKQIILPAVIFL
                     FSIVTAVGFLPIETGVIFGGAYNTSDMIILMKNSLNIGVLIVLIQSAGWLKHSFGNFD
                     NAGGYFFIIVASLIGMDYMISAGDFLMFYIGLELATIPIAALAASEKMKRRSSEAGIK
                     LLLSAAMSSGILLFGISILYGTNGSLNFSSLSADDLNSNLQILAFIFIFTGVAFKISL
                     VPFHLWTADVYEGAPINISSYLSSISKASAVFILLILLFTVFKSILPVWNSVLYIVAV
                     LTMTIGNLFAIRQQNLKRFLAFSSIAQAGFIPLGIIGGTQLGITSVIYFLLIYIFSNL
                     GAFGVVSAISNATGKEKMDDYDGLYQTNPRLSLVMMISLFSLAGIPPVAGFFGKFFLF
                     TAAAVQGYYVLVFIAVMNATISLYYYLLVVKAMFINKNDSPIETIVNDNYTKAGLVIC
                     VLGILLIGFSGAIFEFIESVSKGI"
     gene            8000..8320
                     /locus_tag="PLX80_06530"
     CDS             8000..8320
                     /locus_tag="PLX80_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20486.1"
                     /translation="MALDKGKHNVKEIDGVRCTVIETGVSDERMTFLKNLLELNGLTV
                     KTESESKKIESDPDTFIIGVTDIVFNPVIAVYQRKLRTHEGKIVTAPYWKQLTEESKG
                     WYWK"
     gene            8387..8758
                     /locus_tag="PLX80_06535"
     CDS             8387..8758
                     /locus_tag="PLX80_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY20487.1"
                     /translation="MKLGAFSVSLNVKDINVSKEFYEKLGFNVFGGDINHNYLIMKNG
                     NALIGLFQGMFENNILTFNPGWDENANTVENFDDVRSIQKNFKENGIELQTEADEKTK
                     GPASIVLTDPDGNSILIDQHV"
     gene            complement(8969..9168)
                     /locus_tag="PLX80_06540"
                     /pseudo
     CDS             complement(8969..9168)
                     /locus_tag="PLX80_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            9362..9709
                     /locus_tag="PLX80_06545"
     CDS             9362..9709
                     /locus_tag="PLX80_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY20488.1"
                     /translation="MRRDIFQGIADPVRREIILMIANKNSLNVNSVAERFDISRPAVS
                     KHLKILGECGLIKFTQSGRERFCELELVKMKEVSDWIDPFMILLNERFNNLDNVLSDL
                     NKKSKNINSKTKK"
     gene            9714..10217
                     /locus_tag="PLX80_06550"
     CDS             9714..10217
                     /locus_tag="PLX80_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQY20489.1"
                     /translation="MEKTIYTKDIPNKKIFVKREFNSSPEIVWKAWTESELLDKWWAP
                     KPWTAKTKSMDFTEGGTWMYCMCGPDDEKVWCKVVYKKIDEIKSFEGTDAFCDENGIE
                     STEMPGMYWIVSFKPSENGTTVDVEITFDSEEDLKKIVEMGFQDGFASGHYNLDELLR
                     DLTDIKN"
     gene            complement(10686..11741)
                     /locus_tag="PLX80_06555"
     CDS             complement(10686..11741)
                     /locus_tag="PLX80_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQY20490.1"
                     /translation="MSEIIDQPLIDLPIVEKLTYKDFAPDQDVKWCPGCGDYSILKQV
                     QGLFPELGKKKEEFVFISGIGCSSRFPYYMDTYGMHSIHGRATAIAAGLKASRPELDV
                     WIVTGDGDAMSIGGNHLIHMLRKNFDVNLLMFNNEIYGLTKGQYSPTSKVGLVTKSTP
                     FGSIDQPFNPAELALGAKSTFFARTLDRDPKHMQGVLKKAHAHKGTSFVEIYQNCPVF
                     NDGVFFIYSDKGTKKEESLILEEGKPLVFGENNDKGIKFDGFRPVIVDLNDSNVSVND
                     LWIHDDKDRIKATILASFANENDGVQLPRPFGVFYSIEKPVYEDTMLQQIEDVTKKKG
                     KPSLDEILSGDKTWVIA"
     gene            complement(11746..13602)
                     /locus_tag="PLX80_06560"
     CDS             complement(11746..13602)
                     /locus_tag="PLX80_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQY20491.1"
                     /translation="MKTEEAILERESVVVRFSGDSGDGMQLTGTQFTDTTAFLGRDIS
                     TFPEFPAEIRAPAGTLAGVSGFQVHFGSKEIFTAGDEYDVLVAMNAAALKSDLGNLKK
                     GGIIITNEAGFDAKNLRLAEYSDGVNPLEDGSLSNYEVYTVNVTSLTKEALKDTGLGN
                     KELERSKNMFVLGMLYWIFDQSPDYTINFIKEKFSKKPEIAEANITAIRAGINFGETT
                     EIFNTRFKINPSHLPPGTYRSITGNSALAIGLVIASEKSGLPLFLGSYPITPASDILH
                     ELAKYKYTGVRTFQAEDEISAICSAIGASYGGSLSVTTTSGPGMSLKTEALGLATILE
                     LPLLVINIQRGGPSTGLPTKTEQSDLLQAMYGRHGECPLPILAPCSPSDCFNTIYEAV
                     KLTVEHMTPVIVLSDGYIANGSEPWKFPNSDELENINSVFIDKTNSENGVFLPYKRDE
                     NFVRPRVKPGTKGLEHRIGGLEKKNETGDISYDPLNHELMVNIRQAKVDKIADFIPEI
                     EICTGKEDADILVLGWGSTYGSIKSAVSELDKEGYSVAQAHLRHLNPFPKNLGTLLMN
                     YKNVLIPEINNGQLIRMIRDRFLIPAVGFNKIQGLPFTTTEIKNKIIEILNK"
     gene            13799..>16145
                     /gene="nifJ"
                     /locus_tag="PLX80_06565"
     CDS             13799..>16145
                     /gene="nifJ"
                     /locus_tag="PLX80_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQY20492.1"
                     /translation="MKRKKVTIDGNDAAAYVAHKVNEVIAIYPITPSSPMGELSDAWS
                     AAGQKNIWGTVPMVIEMQSEGGAAGTVHGALQTGSLTTTFTASQGLLLMIPNMYKIAG
                     ELTPTVFHVTARSLSAQALSIFGDHSDVMAVRQTGFAMLCSNNVQEVMDFALISQKAT
                     LESGIPIIHFFDGFRTSHEVMKIEQLNVEDIREMIDDKFVKLHRSRALNPNNPFIRGT
                     AQNPDVYFQGRETVNKYYNEFPAVMKNAMDKFFEITGRQYDLFEYYGSPEADRIIIMM
                     GSGCGTVEETIDELSKNPDEKIGLLKVRLYRPFALNDFVKNIPHTVKYISVLDRTKEP
                     GSIGEPLYLDVIAALNESMNNKNNPFEKMPYVTGGRYGLSSKEFTPAMIKAVFDEMKK
                     DDPKNHFTVGINDDVTFTSLDFDKNFKTESDKQFGAMFFGLGSDGTVGANKNSIKIIG
                     EETDNFAQGYFVYDSKKSGSTTVSHLRFGKEQIKSAYLIESPDFIGCHLFSLMEKFDM
                     LKDIKPGGTFLLNTVYNKDEIFDKLPAKIGQQIIDKKLNFYIIDAFKVAGDTGMGLRI
                     NTIMQTCFFAISGVLPKDEAIQHIKNSIKKTYLSKGDEIINKNFEAVDKTLENLFKVD
                     ITDKKPGEIQIKDTVSSKAPDYVRNVLGKIIEGMGDDIKVSEMPVDGTFPTGTAQWEK
                     RNIAHQIPVWDESICIQCGKCAMICPHACIRIKSYDEKYLKDAPPTFKHMPAKGKEFG
                     VDKVYTIQVAPEDCTGCTLCFEICPVKNKTQVRLKALNMEPQ"
BASE COUNT         5026 a         2815 c         2885 g         5419 t
ORIGIN      
        1 ttcaaaaaag aactaaagat tttattcata tttcagaaaa aaacttccgg aatatgacga
       61 cctgctttcg aataatatta tttttactga gagaactaaa ggcatcggat atctttcaaa
      121 agaagatgca atttcttttg gcgtcactgg tcccacaggc agagcttcag gatttgcttg
      181 tgatgtcaga aagcggttcc cctattcagc ttatgacaga gtagatttta aagaaattat
      241 ttatactgaa ggtgactgct ttgcaagata caaagcaagg atagatgaga tgtgggaatc
      301 gatgaagatc attgagcagc tcattgacaa cattcccgaa ggtgaatata aagaaaaaac
      361 aaaagctgtt attaaattac ccgaaggcga atattatcag agagttgaga cagcccgcgg
      421 agaacttggg gtctatatag taagtgataa taaaaaaagt ccgtacagag tgaaattccg
      481 gtcacccgga ttttcaaatc tttcagcact ggatacaatg gcaagaggaa ataaaattgc
      541 agatctcgtt gcgactatgt caactcttga tctggtgatt ccggatatag acagatagac
      601 agataaactt ttaatttaaa tgaatttcag catatacgat tttacaggtt ttgtcagctc
      661 agtagatcta tggctcagat caaactttcc cccgctggtt gcggacctga ccggatttgt
      721 gatcatcgca atatgctttc ttacttttgt tacacttctc ggtttatcgc ttgtatatat
      781 tgaaagaaaa gtctgcgcat tttttcagca gcgtcttggc cctatgagag tgggattctg
      841 gggcactgca cagactatag ccgacattat aaaactaatt ttcaaagaac cgctcatcac
      901 aaaaaattcc gataaatttc tttttaatct cgctgctttt attgtcatcg ttgcacctat
      961 catggcaata gctgctgtac cttttgctaa aggtcttcat gtacttgatt tcgatatcgg
     1021 actgtttttc attactgcaa tttcatctat cggtgttaca ggtattctac ttgcaggctg
     1081 gtccagtaat aataaatatt ctctcatcgg tgcaatgcgc agtggtgctc agatcgtcag
     1141 ctatgaatta tctgttgtac tttctttatt gacaattatt gtacttacag gaagtcttca
     1201 gctttctgtc atcatagaaa gtcagcgtga cggctggtgg atctttaaag gaaacattcc
     1261 tgcggtcatt gcctttttta ttttcataat tgcaagcact gccgagtcaa accggggacc
     1321 ttttgatatg gctgaagctg aatctgaact tactgcggga tttcatactg aatattcggg
     1381 gatcaaattt gcattcttct ttcttgccga atatataaat ctttttatta tagctgctgt
     1441 cggagctact gttttcttcg gaggatggat gccgctgcat atcggtaatt tcgaattctt
     1501 taataatgta atggacttca ttcctccgat cgtatggttc tttggtaaaa tatcaatgct
     1561 gatatttctt atcatgtggt ttaaatggac tttcccgaga ctgagaatag atcagctcct
     1621 tacactcgaa tggaaatatt tattaccaat aaatttaatt aatctgttac tcatgtcatt
     1681 agtcgcattg aacggatggc acttttgatt tttttaaaaa ttcttaattc ttaattctta
     1741 attttctata ctgggtctaa aaaattacat atcagatatt tatcacggag taaaatctct
     1801 tgtcattgga atgaaagtta ccggacatta ttttatacat ccaaaggaaa ttatcactca
     1861 gcagtatcct gagaataaag atactctgaa aatgtttgac cgcttcagag gtgaagtggt
     1921 tttaatacat gatgaaaata atctgcataa atgtaccggg tgctctgcct gtgaactcgc
     1981 ctgtcctaac ggctcgatag aaattatcag tgacaaagaa atcgatcctg aaacaggtaa
     2041 gtcaacaaaa ataattgata cattcgttta tcatctttca atgtgtactt tttgtaacct
     2101 gtgtatttta tcctgtccga ctgatgcgat caaaatggct cagaattttg aacactcggt
     2161 ctttgacaga tctgaattaa caaaagtttt aaataaaccc ggctctaaaa ttatgcctgg
     2221 tgtgaaatga tctatttaat ttaaaatgag cgaaactgta ttttacatac ttgcatcttt
     2281 tattctgatc ttctcagtgc taactgttac cagcagaaag atactaagga cagcagttta
     2341 tcttttgttt gttttactcg gcacatgcgg attttatttt gcaatggaat tcaacttcct
     2401 agcagccgtc cagcttactg tttacgccgg cggaatcgta gtcataatta ttttttcaat
     2461 tatgctcacc agtgatcttc aggataagct tcctgcggta aagtttaaaa aaatattttt
     2521 ctctctgatt gctgttttat tcggagcagc agtttgtatc actactatat taagttatga
     2581 atttcctgcg atgactacta aagttctcga cagtagtatg cggactatcg gattgagatt
     2641 attaggttat caggaaaacg gatttgtact gccgtttgaa gtaataagca ttttactact
     2701 cgctgcaatg attggttcta ttattattgc aaaaaaagca gacacagaaa attaatttta
     2761 taaataagaa gatatgaccg aagttgtcgg tattcagcat ttcatggttt taagtactct
     2821 gatgtttttt atcggagtct atggattcct tacgagaaag aatcttatca ctattctttt
     2881 atccattgag ctgatactga attccgtcaa cataaatttt gtagcattta ataaatacct
     2941 ttatcccgaa aatctcggcg gtcacttttt tgcaatgttt gtgatcgcag tggcagcaac
     3001 tgaagttgct gtcgcaatag caattattat aagtctttac agaaaattaa aaatgataga
     3061 tgttgaaaat gtgaatgaga tgaagtattg atcattcata ttaatttcaa ttaaatggaa
     3121 aatttttatt acacattaat aatacctgtt atacttttct ttaccttcgt attcacaggt
     3181 ctgcttggca gaaaattcaa acctcttttt tcaggaatgg tgtgtactgc ttctattttt
     3241 atttccacgg cattatcatg gtacacagct taccaatatt tctttgttca aggaaaagtt
     3301 gacggagctt acaaattaat aaatgtcttc aatcttgaat ggctcagatt taatgaaacc
     3361 ctttccatag atataggaat tttacttgat ccgatctctg tgatgatgct cgttgtcatt
     3421 acaactgttt ctctgatggt gcatttatat agtctcggtt atatgaaagg tgatccggga
     3481 ttttcaagat actatgcatt tctttcactg ttcacatttt caatgctcgg actggttttg
     3541 tctgtaaata tttttatgac atatatgttc tgggaactgg tcggagtttc atcttatctt
     3601 ctcattggac attattatga taaaccatca gcagtatcag cttcaaaaaa agccttcatt
     3661 gtcacaagat ttgctgatct cggatttctg attgggattt tacttctctc agcatacgct
     3721 ggttcatttg attttaatga actcacaaat ccacagggaa gtgcaattct tcaaagtgca
     3781 tctgttactt ttatgggact gtcagctgtc acatgggcac ttattttaat ttttatcgga
     3841 tgcgccggaa agtctgcaat gttcccattg cacatctggc tgcctgatgc tatggaaggt
     3901 ccgacccctg tgtctgcact gatacacgct gcgactatgg ttgttgccgg tgtatatcta
     3961 attgccagat tatttccatt atatattttc acttcacctc aggcacttga agtcgttgcc
     4021 tatgttggtg cattcacatc actttttgct gctgtaattg cctgcactca gaccgacata
     4081 aagagagtac ttgcattttc aacaatttcg cagatcggtt atatgatggt tgctcttgga
     4141 gtttcgggat acggcggaga agaaggtctt ggatatatgg cttccatgtt tcatttgttt
     4201 actcatgcaa tgttcaaagc attactcttc ctcggtgccg ggtctatcat tcatgccatt
     4261 catacaaatg aaatctgtga catgggaaat ttgcgaaagt atatgcctgt tactcatatt
     4321 acttttttga ttgcatgctt agcgattgca ggtattcctc ccctctcagg tttttttagt
     4381 aaggatgaaa ttttagttgc agttatagaa aagaatttct ttctctttat tgttcagtat
     4441 attgtagcag ggctgacagc tttttatatg ttcagattat atttcattgt cttctggaat
     4501 aaagaaaaaa aatacgaaca cgaacctcac gaatcaccaa gtataatgct tattactctt
     4561 gtcttcctcg ctgtatgttc agcagtagca ggctttattc cattcagtga atttgtaact
     4621 tcagacggtc ttccgtttgt cactcacatt cacatgaaag ttgctgtacc aactgtaact
     4681 cttgcagtca ttggaatcct cactgcattt tttctttaca aaaaagaaag tacttctcct
     4741 gataaaataa aaatttcact cggattaatt tacagatcgg cttacagaaa attttatata
     4801 gatgaagtat atatgtttgt tacaaagaaa attattttta atatgatctc tgtgccgata
     4861 gcctggtttg acagaaatgt tatcgatagt tccatgaacg gaattgcagc agctacaaat
     4921 ttcatttcag aaaaaataaa atatctgcag tcaggtcagc ttcagcagta tgctttcgtg
     4981 atggttgccg gaatgatctt aatatttctt ttaacagttt atttaataac attataaaaa
     5041 gtaattggat ctgattttct taatattgat tcctgtttta actgtatttg caataatact
     5101 taccgacagt aaagctcagg cacgtattgc agctgtagcc ggaacaggta ttcagttgat
     5161 gctttcaatt tatcttgtga tcctttattt gatggaaaga agctcaggca atattgagca
     5221 gatgcttttc tcgaataaat atgtctggtt cacttcacta aatatttatt ttagtacagg
     5281 tgttgacggg atttctgttt cccttatttt actcaccggt attattactt ttactggtgt
     5341 acttgcttct tgggatgtag attatttata taaagaattt tttatttcct taattgtact
     5401 tgcaacaggt gtattcggat ttttcatttc gcttgacctt tttacaatgt tcttattcta
     5461 tgaacttgca gtaattccga tgtatctgct cataggaatt tggggaagcg gcaagaaaga
     5521 atattcagca atgaaactta cattgatgct gatgggagct tcagcaattt tactcgttgg
     5581 tctgcttgga atttattcat actcatcacc tacaggattg actccgctga catttaacat
     5641 aattgaaatt gctcaaatag atattcctat cgaagcacag agatatttct tcccaatgat
     5701 cttcatagga ttcggagtgc taggtgcttt atttccattt cacacatggt cacctgatgg
     5761 tcatgcttca gcaccgactg cagtatcaat gctgcatgcc ggtgtactta tgaaaatggg
     5821 aggctacgga tgcttaagag ttgcgatgta tttacttcct gaagctgcaa atgaaatggc
     5881 atggatattt attattctga cttcgatcag tattgtttat ggagcattcg gtgcgatagt
     5941 tcagaaagat ctgaaatata ttaatgctta ttcttctgtc agccactgcg gacttgtact
     6001 ttttgctgtt cttatgtcaa atgaaattgc aatgaacggt gctgtgctgc agatgatctc
     6061 tcacggactt atgacagcat tgttttttgc tttgatagga atgatctatg gcagaactca
     6121 tacaagagac attagaaata tgagcggact tatgaagatg atgccttttc ttgcggtgtg
     6181 ttatatgatc gccggctttg catctctcgg acttcccggg cttagcggat tcgtagctga
     6241 gatgacaatt tttgtcggag catttcagaa tgcagatctc tttcacagag gtgttactgt
     6301 tcttgcatgt tcatcgatcg ttatcactgc agtttatatt ttaagagtag ttgcaaaaat
     6361 tttgctcgga cctgctgata aaagtatgcc tgctggaatg accgatgctg tatggaatga
     6421 aaaagctgtg acagtaatac ttatcattgc aatagccgga atgggactgg ctcccctatg
     6481 gctgtctgat atgatattga atagtcttga accggtagta cagaaattaa ctttgctaaa
     6541 atagatttat tataaaatat ttaaaatgac attcgaacaa ttacttttaa tgagacatga
     6601 attactgctg atcatcgttg cagttattat tcttattata gaattgtcaa ccgataataa
     6661 aaataagcag atcattttac ctgccgttat atttttattt tcaatagtca ctgctgttgg
     6721 atttttaccg attgaaactg gcgttatttt cggaggggct tataatactt ctgatatgat
     6781 catactcatg aagaatagtt tgaacattgg tgtattaatt gttctcattc aatctgcagg
     6841 ctggctgaaa cattcattcg ggaattttga taatgccggc ggatattttt ttattattgt
     6901 agcttcactt atcggaatgg actacatgat ctcagcaggt gattttctga tgttctatat
     6961 aggacttgaa cttgctacaa ttccaattgc cgcacttgct gcttcagaaa aaatgaaaag
     7021 aagatcttct gaggcaggca taaaattact tctctcggca gcaatgtcat caggtatttt
     7081 actttttgga atttcaattt tatacggtac gaacggatct ctcaacttca gttctctatc
     7141 tgctgatgat ctcaattcaa atcttcagat acttgcattc atatttattt tcacaggagt
     7201 tgcatttaaa atttcgttag tgcctttcca tctttggaca gcagatgttt atgaaggtgc
     7261 tcctataaat atttcctcat acctttcttc tatttcaaaa gcatcagccg tatttatttt
     7321 actcatactt ttattcacag tgtttaaatc aatactgccg gtatggaatt ctgtattata
     7381 catagtagct gtgctgacaa tgacgattgg aaatttattt gctatcagac agcaaaactt
     7441 aaaaagattt ctcgcatttt cttcgatcgc tcaggcaggg tttattccac ttggaataat
     7501 cggcggtact cagcttggaa tcacttccgt tatttatttt ctgctgatct atattttctc
     7561 aaacctcggt gcgtttggag tagtatctgc aatttctaat gcaacgggca aagaaaaaat
     7621 ggatgactat gacggacttt atcagacaaa tcccagactg agccttgtaa tgatgatctc
     7681 acttttttct cttgcaggta ttccgccggt cgcaggattt tttgggaagt tctttttatt
     7741 tacagccgca gcagtgcagg gatattacgt acttgttttc atcgcagtaa tgaatgctac
     7801 tatatctctt tattattact tactggttgt caaagctatg tttataaata agaatgactc
     7861 acccattgaa actattgtta atgataatta taccaaagca ggtcttgtga tctgcgtact
     7921 cggaattctg ctgatcggat tttcaggagc aatatttgaa ttcatagaat ctgtcagcaa
     7981 aggtatttaa aataatttta tggctttaga caaaggtaaa cataacgtaa aagaaattga
     8041 cggagtccgc tgcacagtaa tcgaaacagg tgtatctgat gagcgtatga ctttccttaa
     8101 aaatcttctc gagctaaacg gactaacagt aaagactgaa tccgaatcta aaaaaataga
     8161 atcagatcct gatactttta ttattggtgt tactgatatt gtttttaatc cggtcattgc
     8221 agtttatcag cgaaagctcc gtacacatga aggaaaaatt gttacagcac cttactggaa
     8281 gcagttaact gaagagagca aaggctggta ctggaaataa attaattttt aattattaag
     8341 aaaattttaa caataaacta ttttacatta atcaaaaaaa atcaaaatga aattaggagc
     8401 attttcagtt agtcttaatg taaaagacat taacgtttca aaagagtttt atgaaaaact
     8461 cggcttcaat gtttttggag gagatataaa tcacaattat ctcataatga aaaatggtaa
     8521 tgcactcatc ggcttatttc agggaatgtt tgagaataat attctgacct ttaatccggg
     8581 ctgggatgaa aatgcaaaca ctgttgagaa ttttgatgac gtaagatcta ttcagaaaaa
     8641 ttttaaagag aatggtattg aacttcagac tgaagcagat gaaaaaacta aaggacctgc
     8701 aagcattgtc ctgactgatc ccgatggcaa ttcaattttg atcgatcagc atgtttagat
     8761 taaaaatact actttaaatt ttaattttaa aattgaattt gtatagattt taagcagata
     8821 taaaagtcat actcattttt ttttatttaa tatgggtaat ctctaataaa gagaaaggtg
     8881 agtttacaaa tttactatta atcatttttt taactgagaa taatcttatt caatttgatt
     8941 cttccttaat ataataactt ggtataaatt aatttatttt atttaagtaa attgcccgct
     9001 ttaattcttt tgctgtgaca atccattctc cgataatcct ttctacattt tttaaagctc
     9061 attataagtt tgtccgtctg ccatacatcc tgacagttcc ggtacctctg caataaatgc
     9121 attatcatct ttactccaat aaatgactgt ttcatattta tatttcattt tatgagttgt
     9181 aataattttt caaatataga atctcatttt aattcttaac tgcatgcaat ctgttttgaa
     9241 aaaataataa tgtttttcta tgcatttctt ctttgtaaaa ttactttaaa aaaagttatt
     9301 gaatatttaa atccttattt ctatatttgt aaccgtttgg ttacatataa aataatttaa
     9361 tatgagaaga gatatatttc agggaattgc agatccggta agaagggaaa ttattctaat
     9421 gatagcaaat aaaaacagct taaatgtaaa ctctgtggct gaaagatttg acatcagcag
     9481 acctgcggtt tcaaaacatt taaagatctt aggtgaatgc ggattgataa aatttacaca
     9541 atccggtaga gagagattct gtgaattaga actcgtaaaa atgaaggaag tgtcagactg
     9601 gatcgatccg tttatgatac ttttgaatga acggtttaat aatcttgata atgtactttc
     9661 agatttaaat aaaaaatcaa aaaacattaa ttcaaaaacc aaaaaataag atcatggaaa
     9721 agacaattta cacaaaagat attccgaata aaaaaatatt cgtgaaaaga gaattcaatt
     9781 cttctccgga aatagtatgg aaagcctgga ctgaaagtga attactcgac aagtggtggg
     9841 ctccgaaacc ctggactgct aaaaccaaat caatggattt taccgaaggc ggaacttgga
     9901 tgtattgtat gtgtggacct gatgatgaaa aagtatggtg caaagtagta tataaaaaaa
     9961 tagatgagat taaaagtttt gaaggcacag acgcattttg tgatgagaat ggaatagaaa
    10021 gtaccgaaat gccgggtatg tattggatag tttcattcaa accttcggaa aacggaacta
    10081 ctgtagatgt ggaaatcaca tttgattcag aagaagatct gaagaaaatt gtagaaatgg
    10141 gatttcagga tggctttgct tccggacatt acaatcttga tgaattgctg agagatttga
    10201 ctgatatcaa aaattgatat ttagtctaaa ttaagaaatt aaaatacggg atgacaaaaa
    10261 attatcatct cgtattttta aaattacatg tttttaatgt atcggaaaat aagagattta
    10321 taagagttat ttaagtataa cttttgtatc attgaaggga aacaaattaa attggaaaaa
    10381 tggataaata caaattatta ttcaagaagc taataattcc taaaaatact tactgaaata
    10441 tttttagttt aatcttatta taattgaaaa ataaaattat gaaaaaattt ataatacaag
    10501 catttacctt taaacatatt aaaggtgaat tcatcggata aaacgggact aaaaaacgaa
    10561 ctctcttcgg aaatagaata caaaaggaat tacttaaagt aaaaagtata ataaattcca
    10621 aaaaaaaatc cccgccaaaa aatgacaggg aaaatatttt gtgttaaata atttaatata
    10681 attactcagg caatcaccca cgtcttatct ccagacagta tttcatccag tgaaggcttg
    10741 cctttcttct tagtaacatc ttcaatttgc tgcagcatgg tatcttcata aaccggtttc
    10801 tcaatagaat aaaatactcc aaaaggtctt ggcaattgaa ctccatcatt ttcgttagca
    10861 aaactagcaa gtatagttgc tttgatcctg tctttgtcat catgtatcca aagatcattt
    10921 acagaaacat tactgtcatt aagatctaca ataactggtc taaatccgtc aaactttatt
    10981 cctttatcat tattttctcc aaacaccagc ggctttcctt cttcaaggat cagtgactct
    11041 tctttctttg ttcctttatc agaataaata aaaaatactc cgtcattaaa gaccggacaa
    11101 ttctgataaa tttctacaaa ggaagttcct ttatgtgcat gagctttttt caatactccc
    11161 tgcatgtgtt tcggatctct gtcaagtgtc cttgcgaaga atgtactttt agctccaagt
    11221 gcaagctcgg caggattgaa aggctgatct atagaaccga atggagttga cttggtaacc
    11281 agtccaactt ttgaagtcgg cgaatactga cctttcgtta atccatatat ttcattgtta
    11341 aacataagca gattcacatc gaaatttttt ctcagcatat gtatcagatg atttcctccg
    11401 atcgacattg catcaccgtc acctgttacg atccatacat ccagttcagg tcttgaagct
    11461 tttagtccgg ctgcgattgc agttgccctt ccgtgaattg aatgcatccc gtatgtgtcc
    11521 atgtaatatg gaaatctcga agagcatccg atcccagata taaatacaaa ctcctctttc
    11581 ttttttccga gctctggaaa taatccctgc acttgtttta atatagaata atctccgcaa
    11641 cccggacacc atttcacatc ctgatccggt gcgaaatctt tataggttaa tttctctaca
    11701 ataggtaaat caattaaagg ctgatcaata atctcactca tattattatt tatttaaaat
    11761 ttcaataatt ttatttttaa tttctgtagt tgtaaatgga agtccctgaa ttttattaaa
    11821 tccgaccgca ggaatcagaa atctatccct gatcattcgt atcaactgac cattattaat
    11881 ttcaggtatc aaaacatttt tataattcat taaaagtgtt ccaagatttt ttgggaatgg
    11941 atttaaatgt ctcagatgag cttgagctac actgtatcct tctttatcca attctgaaac
    12001 tgcactctta atcgaaccat aagtcgaacc ccagccaagc accagtatat cagcatcttc
    12061 tttacctgta caaatttcga tctcaggaat aaagtctgca atcttatcaa cctttgcctg
    12121 acggatattt accatcaatt catgattcag tggatcataa gaaatatccc ctgtctcatt
    12181 ctttttttca agcccgccaa ttctgtgctc cagacctttc gtaccgggct ttactctcgg
    12241 ccttacaaaa ttttcatccc ttttataagg aaggaaaact ccgttttctg aatttgtctt
    12301 gtctataaaa acagaattta tattttcaag ctcatccgaa tttggaaatt tccatggctc
    12361 tgatccgttt gcaatatacc cgtcacttaa aacaatcaca ggtgtcatat gttcaaccgt
    12421 taattttaca gcttcataaa ttgtattaaa acaatccgat ggagaacaag gcgctaagat
    12481 aggaagcgga cattcaccgt gtcttccata cattgcctga agcagatctg attgttcagt
    12541 cttagttggc agacctgttg acggaccgcc tctttgaata tttatgacaa gtaatggtaa
    12601 ttcaagaata gtcgcaagtc ctaatgcttc agtcttcaat gacatacccg gaccggatgt
    12661 agtagttaca cttaagctgc cgccgtaaga tgcaccgatc gctgaacaaa ttgctgagat
    12721 ctcatcttca gcctggaaag ttctgactcc tgtatattta tattttgcaa gctcatgtaa
    12781 aatgtctgat gcaggtgtaa ttggatatga accgagaaat aatggtaatc ctgatttttc
    12841 ggaggcaatc acaagtccaa ttgctaatgc actgttgcct gtaatacttc tgtatgttcc
    12901 gggaggtaaa tgggatggat tgatcttaaa tcttgtatta aaaatttctg ttgtctcacc
    12961 aaaatttata cctgccctga tcgccgtaat atttgcttca gcgatctcag gttttttaga
    13021 aaatttttct tttataaaat taattgtata atcaggcgac tggtcaaaga tccagtatag
    13081 cattccgagt acaaacatat tcttggatct ctcaagttct ttattgccga gtccggtatc
    13141 tttcaatgct tctttagtaa gacttgttac attgactgta tatacttcat aatttgaaag
    13201 tgatccatct tcaagaggat ttactccgtc agaatattca gcaagtctta aattctttgc
    13261 atcaaaacct gcctcattgg ttattattat tccgcctttt tttaggttac ccagatcaga
    13321 ttttaaagct gcagcattca ttgcaacgag cacatcatat tcatctcccg ctgtaaaaat
    13381 ttccttactt ccgaaatgca cctgaaatcc tgaaactccg gcaagcgtac cagccggtgc
    13441 tcttatctcc gccggaaatt ccgggaatgt acttatgtct cttcctagaa atgcagtagt
    13501 atctgtgaac tgtgtccctg taagctgcat accgtctccc gaatctccgg aaaatcttac
    13561 aacaacactc tccctttcta aaatcgcttc ttctgttttc aatttaaata ttcgtaaatg
    13621 atttgtaaat ctgttttgat tttttataaa attaacattt ttttgaatta cattcaccgc
    13681 attaatcaat tcagatttaa tgatattaat catactaatc tatatttcat aattatatat
    13741 tactttgaaa aattttccaa atgaacagaa ttcacttcag aaatttttaa aaatcttaat
    13801 gaaaagaaaa aaagtaacaa ttgacggaaa cgatgcagct gcatatgttg cacacaaagt
    13861 aaatgaagta attgctattt atcctatcac accttcatcg ccaatgggag aattatcaga
    13921 cgcctggtca gccgccggac aaaaaaatat ctggggaact gtcccaatgg taatcgaaat
    13981 gcaaagtgaa ggcggtgctg caggaacagt ccatggagca cttcagaccg gatcgctgac
    14041 aactactttc acagcttcac aaggtctact gcttatgatc cctaatatgt ataagatcgc
    14101 aggtgaatta acaccgactg tttttcatgt tactgcacgg tctctttctg ctcaggcact
    14161 ttcaattttt ggagatcact ctgatgtgat ggctgtcaga caaacaggat ttgctatgct
    14221 ttgctccaat aatgttcagg aagtaatgga ctttgcattg atctcacaaa aagcaactct
    14281 cgaatcagga attccgatca ttcatttttt tgacggattc agaacctcgc atgaagtcat
    14341 gaaaattgaa cagcttaacg ttgaagatat ccgagaaatg attgatgata agtttgtcaa
    14401 actgcataga agcagagcac tcaatccaaa caatcctttt atcagaggca ctgctcaaaa
    14461 tcctgatgta tattttcagg gaagggaaac tgtaaataaa tattataatg aatttcccgc
    14521 tgtaatgaaa aatgccatgg ataaattttt tgagatcaca ggaagacagt atgatctatt
    14581 cgaatattac ggatcacctg aagcagacag aataattatt atgatgggat cgggctgcgg
    14641 gactgtagaa gaaactatag atgagctttc taaaaatcct gacgaaaaga tcggtctact
    14701 taaagtaaga ctatatcgtc cttttgctct gaatgatttt gtaaaaaata ttccgcatac
    14761 agtaaaatat atttcagttc tcgacagaac taaagagccg ggcagtattg gagagccatt
    14821 gtatttagat gtgatagcag ctttgaatga atcaatgaac aataaaaata atccatttga
    14881 gaaaatgcct tatgtgaccg gaggcagata cggattgtct tcaaaagaat ttacaccggc
    14941 tatgataaaa gcagtttttg atgaaatgaa aaaagatgat cctaaaaatc atttcacagt
    15001 aggtattaat gacgatgtta cttttacaag tctggacttt gataaaaatt ttaaaactga
    15061 gagtgataaa cagtttggtg caatgttttt tggactcggg tcagacggaa ctgtcggtgc
    15121 aaataaaaat tccataaaaa taatcggaga agagactgat aattttgcac aggggtattt
    15181 tgtttatgat tcaaaaaaat caggttcgac tacagtctca catctgagat ttggtaaaga
    15241 gcagatcaaa tctgcatatc tgatagagtc acctgacttt atcggctgtc atcttttttc
    15301 attaatggaa aaatttgata tgctaaaaga tataaaacca ggcggtacat ttctacttaa
    15361 tacagtttat aacaaagatg aaatttttga taaactccct gcaaagattg gacagcagat
    15421 cattgataaa aaactaaatt tttatattat tgatgctttc aaagttgcag gtgatacagg
    15481 catgggtttg agaattaata cgatcatgca aacatgtttc tttgctatct caggagtact
    15541 tccaaaagat gaagctattc agcatataaa aaattctata aagaaaactt atttatccaa
    15601 aggtgatgag atcattaata aaaattttga agcagtagat aagactctgg aaaatctttt
    15661 taaagttgat atcacagata aaaagccggg agaaattcag atcaaggata ctgtatcctc
    15721 aaaagcacct gattatgtaa gaaatgtttt agggaaaatt attgaaggaa tgggtgatga
    15781 tataaaagta agcgagatgc ctgttgacgg gaccttccct accggcactg ctcagtggga
    15841 aaaaagaaat attgctcatc agatccccgt ctgggatgaa agtatttgta ttcagtgcgg
    15901 caagtgtgca atgatctgcc ctcacgcttg tatcagaatt aaaagttatg atgaaaaata
    15961 tttaaaagat gctccgccta cttttaaaca catgcctgca aaaggaaaag aatttggtgt
    16021 ggacaaagtt tatacaatac aggtagctcc cgaagactgt accggatgta cattatgttt
    16081 tgaaatttgt ccggtgaaaa ataaaacaca ggttcgactc aaagcactga acatggagcc
    16141 gcagc
//
LOCUS       DAORVQ010000059        16103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1562_length_16103_cov_4.768445, whole genome shotgun sequence.
ACCESSION   DAORVQ010000059 DAORVQ010000000
VERSION     DAORVQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 16103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16103
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_16103_cov_4.768445"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PLX80_06570"
     CDS             <1..1339
                     /locus_tag="PLX80_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20493.1"
                     /translation="SLLFLAVLLFIISDFKYFNRKNSKILYFIPLLFLLWANIHMGVL
                     AGALLLFIFLLSEIIIYIKPVKFSSKDIPALSLSEIKLLGIIFIISMIVMLINPNGIA
                     TYFYAYGHTKMKLLETINEWRSPFDEMFSSGFVTMIYKVFLFGGLLVLYYSFKKKDLF
                     TGMLFIVFAVYSVRAVRFTVDYIIIITVFIGLALFFILNNLKSEGIKNFINESPLPKV
                     ILEVILLFFIFNIPNDKLYLEHLKYYRVSGFGINSDFIPVQMFDFMKENKIPEIGKRP
                     LNHFGSGGYLVWNFPESKNFIDSRNLNDEIFNEYNTLIGKGPGFEKKLNEFDFDYSLY
                     LAPDLVRAPNEMESTIISYYSKNPDQWKLVFWDDKSFLYLKNDPKFQDIIDKYEYKYI
                     TPYNYMYQKKSIENAVINDPDRVKNEIDRKLKEEQNGLIINTINNTFSKSLNK"
     gene            1371..3722
                     /locus_tag="PLX80_06575"
     CDS             1371..3722
                     /locus_tag="PLX80_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQY20494.1"
                     /translation="MKTLLTLLLTILSFSISESQIVPISDLKMNDANGLPLLNGQFKT
                     VTGIVTVANEFGGPSYIQDVTGGLGVFYTEFSSAISIGDSIIVTAKMGHFSGLSELVY
                     STDGGTPSFSVISNGNAVPEPVVATISQFNNQSFASVEEYEGKLVRINGITITGSGTF
                     AANTNYTITDATGTGQLRIDNNSNLVGTIIPTGEVDCIGACGQFDSSPPYSSGYQLLP
                     RFTADILQSGGPIILTLPAETNITPNSVTLSWTTQTAGDSKLKYFRSDSINQPMVFTD
                     SMYSATLSTNHSFNLSNLSAGKIYCAVVTSTNGSGTSVSSTKYFSTASNIASTGKWEI
                     YFNKAVDNSYAMPNNVAVGNADLKIRLGQRIDSAVYSIDFAMYSFNEVTQVKDKLINA
                     LIRGVKIRMVYDHRDGNTQALVQDLINAGISVSQRPQSTNIMHNKFFIFDARNDNSFS
                     DDWLWTGSANITNAQFYDDAQNVIFIQDQALCNTYTREFEEMFGSHGNTANPSQAKFG
                     VEKNDNTPHLFIINGKNVECYFSPSDNTANVFDNLIGNNTDNSIFFCAFAFTRFQIAN
                     KMKTQFNPPNKMVRGVFDDGNGTDPSSVYPEMKGIGGNFPWNPPAPVYLDNQPGTMHS
                     KYMLIDADMPSSDPIVETGSYNYSTAATTGNDENFLLLFDSLIANQYLQDFTKRYSLA
                     GGTISVEQISSVIPGGFTLSQNYPNPFNPKTVIEYSIPASDFINLKIYNILGHEIKTL
                     VNENQTPGSYKVTFDGSNLPSGVYYYRLETKGNISTKSMLLIK"
     gene            3819..7157
                     /locus_tag="PLX80_06580"
     CDS             3819..7157
                     /locus_tag="PLX80_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HQY20495.1"
                     /translation="MKKIKIPVCVFIFLGLIFLITGKTFSQANAVFGQNKVQYKTFDW
                     SYIQTKHFDIYFSKGGFMLAEFTAHTIEEALRNMTDNIDYNISNRIPVIVYNSHIDFQ
                     QNNVVDEFLSEGIGGVTELFKNRINIPFEGDYEKFRHVIHHELLHAFMNDMYYGGSIQ
                     NIISKNISLQFPLWFSEGMAEVQSRYGLDLETDMFMRDATINNYVPELAYLGGYFAYR
                     GGQSFFAFLADTYGEDKLGVLMNNIKAYGDVDKGFLKTYQVPLPELGEKWHKYLKKMY
                     WTELNIREDVSDFAKQLTDHERDGGNYNISPVISPKGDKFAFISNKDDHFDVYIASTK
                     SGKILKKLIEGNLSTEFEELHILTPGLAWSPDGRKIAISVKSGGSDVIYVIDIFSDDK
                     VEYPIDFDGINYIGWSPKGDKVAFIGIKEAYADLYVYDIKTKKLEALTSDIFTDANPT
                     WAHDGKSLFFTSDRGSYTNPSEIPHDFKMWEYDYSRSDYYSIDISTKEIKRLSHSKYA
                     KESYAQVNQDGNKILYVSDKNGIKNLYVRERDSSGNVTDRPVTNSLNPIDQLSLSKDG
                     KKALFVSLYNGGYNVFSLDNPFDIDLNLDELKPTDYSLRLEEFDTRFGMRQEFKFIDS
                     ADLAVNDTIDAFDTLFIARDSADFQNDTMAVGEIVEDSLSLDRVDSLKNEIEKVIQKD
                     TSFLSLNEKEKNENDSIKTYGDDVKVSFETPKNMSSSKKDSSYFGNSNFKISNNVNED
                     GSYKIKNYKIKFTPDLVYGNADYTTFYGLRGVAQIALSDVLGNHRIYIQTSLIIDLKN
                     SDYAVAYLYLPKRIDYGFQLYHTARFVEFGNQFVNFLYRYRTVGGTVSASLPISRFKR
                     IEGNLGLQYITRENLDLTIEPVETQTLLLPYFSFIHDNTTFGYTAPVSGTRYNITFGG
                     SPNLGEDGIGFASTLLDFRHYLKLNNDYSFAMRLSGGVSYGKNPQRFFIGGTENWIDP
                     TYENYYLPVSDIQDFAFSSPGLPLRGFNYDRLSGSKYSILNLELRFPLFRYLIFGALP
                     LGFANIEGVTFIDAGTAWSENEALKLTTTENGKTVTNDLLLGTGFGARANIFGFPLKF
                     DLAWNYNFDKFSSPKYYISLGYNF"
     gene            complement(7234..7854)
                     /gene="rplI"
                     /locus_tag="PLX80_06585"
     CDS             complement(7234..7854)
                     /gene="rplI"
                     /locus_tag="PLX80_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQY20496.1"
                     /translation="MKVILKQDHDLLGDDGQIIDVKNGYARNYLIPNGIAIMANKSNL
                     KSHEEITRQKSRKTDKVAADSNKLADVLSTHSFEFKVKAGEDDRLFGSITSQTIHELL
                     EEKGFVTVERKKILLKEPIKSLGEHEVEIKLPQSVIAKIKINVVKEGSDQEEKVSEEI
                     ITETEKNQEVNGEIQTENNSEVKAEEVNIPDENQDEINEETKQETE"
     gene            complement(7926..8399)
                     /locus_tag="PLX80_06590"
     CDS             complement(7926..8399)
                     /locus_tag="PLX80_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQY20497.1"
                     /translation="MADYKMPELNAVVIAGNLTKDPIFRQTNSGTPVVNFSIASNRRF
                     KDKNDEWKEDVCYVGIVAWNRLAESCKDRLRKGNAVLVDGELQSRTFKTDDGSNRTIV
                     EIKARRIQFLNKKGSESDYQDSEVHTEREEDVTSFTDDSFDSLLSPEDSDLLKDK"
     gene            complement(8441..8905)
                     /gene="rpsF"
                     /locus_tag="PLX80_06595"
     CDS             complement(8441..8905)
                     /gene="rpsF"
                     /locus_tag="PLX80_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQY20498.1"
                     /translation="MSKKFYESYVIFDGNLDDSAIEDLIKKYESLFAKNEIEIHNVVR
                     IGRKRLAYEIEKKQNGFYVCFEIYSPSDLITKFERAYVLDESILRYLTIFVSDKTRKE
                     KDEHFKFKAIMQQKFEDTKAKELKEEEEKASEKSESKKEEVQEVNKKVLENN"
     gene            complement(9020..9102)
                     /locus_tag="PLX80_06600"
     tRNA            complement(9020..9102)
                     /locus_tag="PLX80_06600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9066..9068),aa:Leu,seq:gag)
     gene            complement(9198..9767)
                     /locus_tag="PLX80_06605"
     CDS             complement(9198..9767)
                     /locus_tag="PLX80_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HQY20499.1"
                     /translation="MENLLSIFLGIGLSAACGFRIFIPFLILGITSSMGLLDLDPNFE
                     WISSTLFIIIFAAFSLAEIIGYFNPWIDNMLDTVATPLSIIAGIILSLSVITGFDPII
                     HWAIAIIIGGGITANFQFLSVKARSVSSVFSSGFGNPVISGLELLISIMLSILSILFP
                     LIAFLFIIVLIISLFAILRNAKARLNEKF"
     gene            complement(10788..12155)
                     /locus_tag="PLX80_06610"
     CDS             complement(10788..12155)
                     /locus_tag="PLX80_06610"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY20500.1"
                     /translation="MRKFIMDINPSTGELIEKIKCSSPGEIASGVKLARKAFKVWSKT
                     PLKKRILLFKKIARDLEKEKKHIGELITKEMGKVYSSAVGEVAGTAYAIEENIKLVEK
                     AAVTEIHKEGLLITEVHRMPLGVVAVITPWNFPVGMPESLLTPAILMGNTVVFKPSEN
                     TPLTGKAIYNIFNRYLPKGVINLLQGAEEVGEALLSTDIDMVSFVGSQAVGKKIMEAC
                     SKKLNRLVLELGGKDPMIVMKDADLEKAVNYAVNGSLRNSGQVCVSVERIYVDQSIFG
                     KFEKLVLEKVKTYKVGDGFDEVDMGPMVSEAQRSHVIGQIEEAKKKGAKILFGGSIID
                     GDGFFIEPTVISNLNHKHDIMMNETFGPVVCLQKVKNVEEAVEKANDSVFGLGATVWT
                     KSTDKGRKIAEQIESGMIGVNQGIGGVSGTPWVGIKQSGFGFLGSVEGVKQFTVPKKI
                     SYKKK"
     gene            complement(12178..13221)
                     /locus_tag="PLX80_06615"
     CDS             complement(12178..13221)
                     /locus_tag="PLX80_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY20501.1"
                     /translation="MKIVVTGGAGFLGYHISQKIKRITDFKDCTFFDIAEFEKDEYDP
                     AIKCIYGDVRDKAQMDEMMKNVDVIIHCAAALPLWKKKDIFETNVDGTRNVLESALKN
                     KVNRVIHISSTAVYGVPDVHPLFEDHQREGVGPYGTSKVLAEEVCEEIRKKGQPICIL
                     RPKSFIGTARLGVFQILYDWVESGVKIPIIGNGTNRYQLFEVEDLVDAILQAASLPLD
                     KINDTFNVGAKEFKTVNEDVGAMCVYAGTGSKVMKTPASLIKPLLRFFEILNVSPLYK
                     WIYGTADTDSFVSIDKAMKQLEWNPKYSNSEALIRSYQWYLDNKHKIQQGTGVTHRIA
                     WKQGVLSFFKKFL"
     gene            complement(13244..13972)
                     /locus_tag="PLX80_06620"
     CDS             complement(13244..13972)
                     /locus_tag="PLX80_06620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY20502.1"
                     /translation="MTDKVAIVTGGTGALGRVVSEKLAHLGIKIYIPSRSLDTFNKVF
                     DNSQNENSVDFNLKKIFSFECDITNEASVIEFVKNVAVQEKGKIDFLINTAGGIDSPV
                     NTGELSTPAFMKMFDLNFMSAFYFSREVLNVMLKNNFGRIISFGSKSATELTPKKFAY
                     SYSKLGVIQLMNILGEEYKKNNIRCNTIIPGIIDTPSNREWGTAEDVKSWTKPGDIAE
                     IISDVISDKYISLRDSELKLYNSF"
     gene            complement(13978..15144)
                     /locus_tag="PLX80_06625"
     CDS             complement(13978..15144)
                     /locus_tag="PLX80_06625"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQY20503.1"
                     /translation="MNILKGIVNKSRRKVIGILSGTSIDAVDVVLTEIYGSGIKSEIR
                     ILEFDSFPIDKKVRNLILRCSSEKTGNTADICKLNFIIGRLFGIKINEFLKKNKISPN
                     EIDLTGSHGQTIFHYPVKNNQYGYDSKSTMQIGDLSVIANITGVTTIGDFRTADVSVN
                     GDGAPLVPYLDYILSRKINCSSVFLNIGGISNITYIKKNCKLNEVIAFDTGPGNLLID
                     LISKKYFNKKFDKDGKIAGKGNINSNLLEKIVTKDKFVKKPSPKSTGREYYNEEFIES
                     IVKKNSYDPYDLMATFNFFTAYAVNYNIKKFKTDKIFVSGGGSNNLTLMKNLRNLFEG
                     TDLEILNLNGINSSNKEAVLFAVLANELISGNKTNIISVTGADKNVFLGKICIA"
     gene            15391..15735
                     /locus_tag="PLX80_06630"
     CDS             15391..15735
                     /locus_tag="PLX80_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY20504.1"
                     /translation="MEEFSIKTKQHDNVSILDLEGYLDAHTSVELEKCLEKIISENKF
                     KIIVNFEKLSYISSAGLGVFMAFIETARNNAGDIKLCAMSDKIYNIFDMLGFPILFEI
                     YKEENSALEKFS"
     gene            15766..>16103
                     /locus_tag="PLX80_06635"
     CDS             15766..>16103
                     /locus_tag="PLX80_06635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY20505.1"
                     /translation="MFKSSTKNLSLLRKFIEDKALAFGFDESSINQIILSVDEACTNI
                     IKHAHKYNEKEIIEVDLDSNENQFIIKLNYKGKAFDPNNISNPDMNDYFNKFKVGGLG
                     VPIMKKFMNK"
BASE COUNT         5403 a         2658 c         2377 g         5665 t
ORIGIN      
        1 gtctttactg tttctcgctg ttcttttatt tatcatatcc gattttaaat atttcaacag
       61 aaagaattca aaaatactgt acttcattcc tcttcttttt ttattatggg caaatattca
      121 tatgggagtt ttggcaggtg cattactgct gttcattttt ctgttaagtg agataatcat
      181 ttacataaag cccgtaaaat tttcatcaaa agatatacct gcattatcac tttctgaaat
      241 aaaattactt ggcataattt ttattatctc aatgatcgtg atgctgataa atccaaacgg
      301 cattgcaaca tatttttatg cttacggaca taccaagatg aaactgcttg agacgataaa
      361 tgaatggaga tctccgtttg atgaaatgtt cagctcggga tttgtgacaa tgatctacaa
      421 agtatttctc ttcggaggat tactggttct atattattcc tttaagaaaa aagatctttt
      481 tacgggaatg ctctttatcg tttttgcagt atattctgtc agagctgtaa ggtttactgt
      541 tgattatata attattataa ctgtctttat cggactggca ttatttttta ttttaaataa
      601 tttaaagagc gaaggtataa aaaatttcat caatgaaagt cctttgccaa aagtaatact
      661 ggaagttatt ttactgttct ttatttttaa tatcccaaat gataaacttt atctggagca
      721 tctaaaatat tacagagtat caggatttgg aattaattca gatttcattc ccgttcagat
      781 gtttgatttt atgaaggaga ataaaattcc tgaaattggt aaacggccat tgaatcattt
      841 cggcagtggt ggttatctgg tatggaattt tcctgaaagt aaaaatttta tcgacagcag
      901 aaatctgaac gacgagatct tcaatgagta caatacgctg ataggaaaag gacccggctt
      961 tgaaaagaaa cttaatgaat ttgattttga ttactcactg tatcttgcac ctgacctggt
     1021 aagagctcca aatgaaatgg aatctacaat tatttcttat tacagtaaaa atccggatca
     1081 gtggaaactt gtattctggg atgataagtc ttttttatat ctgaaaaatg atccgaagtt
     1141 tcaggatatt atagataaat atgaatataa atatattaca ccttataatt acatgtatca
     1201 gaagaaatca attgaaaatg cagtgataaa tgatcctgac agagtaaaaa atgagataga
     1261 caggaaatta aaagaagagc aaaatggatt gataattaat actataaata acactttttc
     1321 aaagagcctg aataaataat aatataaaaa ttaacttaaa aaatatataa atgaaaacat
     1381 tattaacttt gttattaacg attctgtcat tttcaatatc agaatcacag atagtaccta
     1441 tttcagatct aaaaatgaac gatgccaatg gattgccatt acttaacgga caatttaaaa
     1501 ctgtaaccgg aattgtaaca gtagcaaatg aatttggagg tccttcttat atacaggatg
     1561 tcacaggtgg tctaggagtt ttttatactg aattttcttc cgcaatttca attggtgaca
     1621 gtataatagt aactgccaaa atgggacatt tcagcggact ttcggaactt gtctattcta
     1681 ctgacggagg aacaccttca ttctcggtaa taagtaacgg aaatgcagtt cctgagccgg
     1741 ttgtagcgac aatttcacag ttcaataatc agtcatttgc aagtgttgaa gaatatgaag
     1801 gaaaactggt cagaataaac ggaattacaa ttacaggatc cggtacattt gcggcaaata
     1861 ctaattatac tattacagac gcaacgggaa ccggacaact tagaattgat aataattcaa
     1921 acttagttgg cacgataatc ccaactggtg aagttgactg tattggagct tgcggacagt
     1981 ttgattcatc tcctccatac agttcaggat atcaactgct gccgagattt actgctgata
     2041 tacttcagtc cggcggacct attattctca cacttcctgc agagacgaat ataactccaa
     2101 acagtgtaac actttcatgg actactcaga ccgcaggcga cagtaaatta aaatatttcc
     2161 gatctgattc tattaatcaa ccaatggttt ttacagattc aatgtacagt gcaacacttt
     2221 ctacaaatca cagctttaat ctgagcaatc tttcagcagg aaagatctat tgcgcagttg
     2281 ttacttctac aaatggttca ggtacaagtg tcagttcaac aaaatatttt tcaactgctt
     2341 ctaatattgc ttcaacagga aaatgggaaa tttattttaa taaggcagtt gataattctt
     2401 atgctatgcc aaacaatgtt gccgtcggta atgcagatct aaaaatcaga cttggtcaga
     2461 gaatagactc agcagtatat tctattgatt ttgcaatgta ttcttttaat gaagtgactc
     2521 aggtaaaaga caaactgatc aacgccttaa taagaggtgt aaagatcaga atggtttatg
     2581 atcacagaga tggaaataca caggcacttg ttcaggatct tatcaatgca ggaataagcg
     2641 tttcccagag accccagagt acaaatatta tgcacaataa attttttata tttgacgcac
     2701 gaaacgacaa ttcattttcc gacgactggc tttggacagg ttcagcaaat ataactaatg
     2761 cgcagttcta tgatgacgct cagaatgtga tcttcattca ggatcaggca ttatgcaata
     2821 catacacaag agagtttgaa gaaatgttcg gttcacatgg aaatacagcc aatccttcac
     2881 aagcaaaatt cggagttgag aaaaatgata atacacctca tttatttatc attaatggta
     2941 aaaatgtaga atgttatttt tcaccttctg ataatactgc aaatgtattt gataacttga
     3001 ttggaaataa cacagataat tctattttct tttgtgcatt tgcatttaca agatttcaga
     3061 ttgcaaataa aatgaaaact caattcaatc caccgaacaa aatggtaaga ggagtttttg
     3121 atgatggtaa cggaaccgat ccatccagtg tgtatcctga gatgaaaggt attggcggta
     3181 atttcccatg gaatcctcct gctccggtat atttggataa tcaacccggt acaatgcata
     3241 gtaaatatat gcttattgat gctgatatgc cttcaagtga tccaattgtt gaaacgggat
     3301 catataatta ttcaacagca gcaactaccg gcaacgatga gaacttcctg ctactttttg
     3361 attcattaat tgcaaatcaa tatctgcagg attttacaaa gagatattca ctggcaggtg
     3421 gaacgattag tgtagaacag atctcttcag taataccagg tggatttaca ttaagtcaga
     3481 attatccgaa cccattcaat ccaaagaccg taatcgaata ctctattccg gcatcggatt
     3541 tcataaatct gaaaatttac aatattcttg gacacgagat aaaaacatta gtaaacgaga
     3601 atcagactcc cgggtcttac aaggtaacat ttgatggaag taatcttcca agtggagttt
     3661 attattacag attagaaaca aaaggtaata tttctacaaa gagtatgctt ttgataaagt
     3721 aataataaat tatattaatt tattaaagtg tgaaactatt taaatttgat gatgttcaaa
     3781 aaacagacac atttcaagtt taaataaatc aggagattat gaagaaaatt aaaattcctg
     3841 tatgtgtatt tattttttta ggtttgattt ttcttataac cggaaaaaca ttttcacagg
     3901 caaatgcagt gtttggtcag aataaagttc aatacaaaac tttcgactgg agctatattc
     3961 agaccaagca ttttgatata tatttcagca aaggtggatt catgcttgct gaatttactg
     4021 cacatactat agaagaagca ctaagaaaca tgacggataa tatagattat aatatttcca
     4081 acagaatacc tgtaatagtt tacaattcac atattgactt tcagcaaaac aatgtagttg
     4141 acgaatttct ttccgaagga attggcggtg ttactgaatt gtttaaaaac aggatcaaca
     4201 ttccatttga aggtgattac gaaaagttca gacatgttat tcaccatgaa cttttgcatg
     4261 catttatgaa tgatatgtat tacggaggtt ctattcagaa tattatttcg aaaaatattt
     4321 cccttcagtt tccattgtgg tttagtgaag gtatggctga agttcagtca agatacggac
     4381 ttgaccttga gactgatatg tttatgaggg atgctacaat aaataattat gttccagaac
     4441 tagcatatct tggcggatat tttgcttaca gaggcgggca aagttttttt gcttttctag
     4501 cagatactta tggcgaagat aagttaggtg tgttaatgaa taatataaaa gcttatggtg
     4561 atgtagataa aggcttttta aagacttatc aggtaccatt accagaactt ggtgaaaaat
     4621 ggcataagta tctaaaaaaa atgtattgga ctgaattaaa cataagagaa gatgtatctg
     4681 actttgcaaa acaacttacc gatcatgaaa gagatggtgg aaattataat atttcacctg
     4741 ttatttctcc taaaggagat aagtttgctt tcatttcaaa caaggatgat cattttgatg
     4801 tttatatagc aagtacaaaa tccggtaaaa ttcttaaaaa attgattgaa ggaaatttat
     4861 caactgaatt tgaagagctg catattttaa cacccggact tgcctggtca cctgacggca
     4921 gaaaaattgc tatcagcgta aaatcaggtg gttcagatgt aatttatgta attgatattt
     4981 tcagtgatga taaagtagaa tatccaatag attttgacgg aataaattat attggctggt
     5041 cacctaaagg tgacaaagtt gctttcattg gtataaaaga agcatatgct gatctttatg
     5101 tgtacgatat aaaaacaaaa aaacttgaag cactaaccag tgatatattt actgatgcta
     5161 atccaacctg ggcacacgac ggaaaatctc tattttttac ctcagacaga ggtagttata
     5221 caaatccttc tgaaatacct catgacttta aaatgtggga atatgattat tccagaagtg
     5281 attattatag tatagatatt tcaactaaag aaatcaaaag attaagtcac tcaaaatatg
     5341 ctaaggaaag ttatgctcag gtaaatcaag acggaaataa gatcctttat gtctctgata
     5401 agaatggtat taaaaattta tatgtaagag aaagagattc ttcaggaaat gtaacagaca
     5461 gacctgtaac aaattctctt aatccgattg atcagcttag tttatctaaa gatggtaaga
     5521 aagcattatt tgtatcttta tataatggag gttataatgt tttctcttta gataatccat
     5581 ttgatattga cctgaattta gatgaactaa agccaaccga ttattcatta agacttgaag
     5641 aatttgatac acgttttgga atgagacagg aattcaaatt tatcgacagt gcagatcttg
     5701 ctgtgaatga tactattgat gcttttgata cattatttat agcccgggat tctgcagact
     5761 ttcaaaatga tacaatggca gttggagaaa tagttgaaga ttctcttagt ttagatagag
     5821 tagattcatt aaagaatgaa attgaaaaag ttatacaaaa agacacctca tttctttctt
     5881 taaatgaaaa agaaaaaaac gaaaacgata gcattaagac ttatggagac gatgtaaaag
     5941 ttagttttga aactccaaag aatatgtctt caagcaaaaa agattcttct tattttggaa
     6001 actcaaattt caaaatatcc aataatgtaa atgaagatgg atcatacaag ataaaaaact
     6061 acaagatcaa attcacacct gacctggtat atggtaatgc cgattacacg acattttatg
     6121 gtctaagagg agtggctcag atcgctctta gtgatgtatt gggaaaccat agaatttata
     6181 ttcaaacttc acttattata gatcttaaaa acagtgatta tgctgttgct tatctatatt
     6241 taccaaaacg aattgattat ggattccagt tatatcatac tgcaagattt gttgaatttg
     6301 gaaatcagtt tgtgaatttc ttgtatagat atcgtacagt tggtggaacg gtttcagcat
     6361 cattaccaat ttcaagattc aaaagaattg aaggtaattt aggtttgcag tacataaccc
     6421 gagaaaatct ggatttaaca atagagccgg tagaaacaca aacactttta cttccctatt
     6481 tcagttttat tcacgacaat acaacttttg gttatacagc tcctgtttct ggaacaagat
     6541 acaatattac ttttggaggt tcaccaaatc taggtgaaga tggaattgga tttgccagta
     6601 cattacttga tttcagacat tacctaaaat tgaataatga ttattcattt gcaatgagac
     6661 tttcgggtgg agtaagttat ggaaaaaatc ctcagcgttt tttcattgga ggaacagaga
     6721 actggataga tccgacttat gaaaattatt atctgcctgt ttccgatata caggattttg
     6781 ctttctcttc acccggttta ccattaagag gatttaatta tgacagatta tcaggatcta
     6841 aatattctat tctaaacctt gaacttagat ttccattatt cagatatttg atttttggtg
     6901 ctttaccact tgggtttgca aatatagaag gggtaacttt catagatgca ggcactgcct
     6961 ggtcggaaaa tgaagctctg aaattgacaa ctacagaaaa tgggaaaacc gttactaatg
     7021 atctcttact tggaactgga tttggagctc gggcaaatat tttcggattc cctcttaaat
     7081 ttgaccttgc ctggaattat aattttgata aattttcaag tcctaaatat tatatctctt
     7141 taggttataa tttttaaaga atgaaatatt caatttaaac acaaaaaacc cggcaagtac
     7201 cgggtttttt tttataaaat atttaattat tttttattca gtttcttgtt ttgtttcttc
     7261 attgatttca tcttgatttt catcaggtat attcacttct tcagctttta cttctgagtt
     7321 attttcagtt tggatttctc catttacttc ttgattcttt tcagtctcag taatgatttc
     7381 ttcagaaact ttctcttcct gatctgaacc ttcttttaca acattaattt ttatttttgc
     7441 aattacagac tgtggaagtt taatctcgac ttcatgttca cctaaagatt ttataggttc
     7501 ttttaaaaga attttttttc tttctacagt aacaaaacct ttctcttcaa gaagttcatg
     7561 aattgtttgt gaagtgattg atccaaagag tctatcatct tctccggctt taactttaaa
     7621 ttcaaatgaa tgagtactta atacatcagc cagtttgttt gaatcagctg caactttgtc
     7681 agttttccta cttttctgtc ttgttatttc ttcgtgactt ttcagatttg atttatttgc
     7741 cattatagca attccatttg ggatcaaata atttctcgca tatccgtttt ttacatcaat
     7801 tatctgaccg tcatcaccta aaagatcatg atcttgtttt aatataactt tcattttatt
     7861 atttatttat aaagaaaagg gtgaaaaatt ttctcacccc aatattttat tacaaaaaac
     7921 ttattttatt tatctttaag taaatcagaa tcttcaggag agagtaatga atcaaaagaa
     7981 tcatcagtaa aagatgtaac atcttcttct ctttctgtat gaacttctga atcttggtaa
     8041 tcagattcag atcccttttt atttaaaaat tgaattcttc tggctttgat ctcaactatt
     8101 gttctgttag atccgtcatc tgttttaaaa gttctgctct gtaattctcc atcaactaaa
     8161 actgcattac ctttccttag tctgtcttta caactttcag ctaaacggtt ccacgctaca
     8221 atcccaacat agcaaacatc ttctttccac tcatcatttt tgtctttaaa tcttctgtta
     8281 ctcgcaattg aaaaatttac aacaggagta cctgagtttg tttgtctgaa aatagggtct
     8341 ttcgttaagt ttcctgcaat cactactgca ttcagttccg gcatcttata gtcagccata
     8401 atttccctct actgttaatt agattttaaa atctttatag ttaattgttt tctaacactt
     8461 ttttatttac ttcctgaact tcttcttttt ttgattcgga tttttcagaa gctttctcct
     8521 cttcctcttt taactcttta gctttagtat cttcaaattt ttgctgcata atagctttaa
     8581 acttaaaatg ttcatccttt tcttttcttg tcttatcaga tacaaaaatt gtgagatatc
     8641 tgagaatact ttcatccagg acatatgctc tttcaaactt ggtaattaga tcggaaggag
     8701 aataaatttc aaaacaaaca taaaatccat tttgcttttt ttcaatttca tatgctaatc
     8761 tttttctgcc gattctcaca acattatgaa tctcaatttc atttttggca aatagtgatt
     8821 catatttttt aattaaatct tcaattgcag aatcatctaa attaccatca aagataacat
     8881 aggattcata aaactttttt gacaaaattt cctaagtatt aatttataaa gctacaaata
     8941 tatcacgatt aaagctaata atcaagatat ttaaaatagc tttaatccta aaaaatgtag
     9001 ttacaataat tttaaaaagt gcggaaggcg ggacttgaac ccgcatgcct tgtgagcgcc
     9061 accccctcaa gatggtgtgt ctgccagttc caccacttcc gctaagaagt gcaaaattaa
     9121 atgatattga gataaattta aatactaatt ttttactttt tgaaaataat ttgcaatttc
     9181 aaaaaaaatc agatcagtta aaacttctca tttaatctcg cttttgcatt tcttaatatt
     9241 gcaaaaagtg aaataatcaa aactataata aataaaaaag caattagagg gaaaagaata
     9301 gataaaatag atagcataat tgatattaat aattctaatc cggatatgac gggatttcca
     9361 aatccacttg aaaaaaccga agaaacagac ctggctttta cagaaagaaa ttgaaaattc
     9421 gctgtaatac caccacctat tatgattgcg attgcccaat gaattattgg atcaaatcct
     9481 gtaataactg ataatgataa aattatacct gcaataattg atagcggagt agctacagta
     9541 tcaagcatat tatcaatcca aggattaaaa tatccgataa tttctgcaag agaaaatgct
     9601 gcaaatatta ttataaacaa tgttgaagat atccattcga aattaggatc taaatccagt
     9661 aagcccatag aacttgtaat tccaagtatc agaaacggaa taaagattct aaagccacag
     9721 gcagcactta aaccaattcc taaaaatata cttagtaaat tttccaattt taaatcgtat
     9781 ccgtttttta atttttaata ttaaaaattt cggtcaaaca ttcagttgat aaaataatat
     9841 tacataagaa atttttatga aatttcaaaa ataatgataa gcagataaaa ctttatttat
     9901 tgtattgaga ttattttttt atttgaaatt tttcaggggt aaaaccaata tgattttttt
     9961 aattaacaaa ctaaaaatgt tcggttcaat tcaatttatt taaatacaat caattctgta
    10021 aaccatttgc gtttacatgt cattattatc aacttttcaa gcaagttata aaaatcaata
    10081 ctagtttagg tatattcata gatcatctgg agaatttaat gaattgcaac ttttcgcaat
    10141 tagttataac ttgcaaatca tttgcttttt atgttaaaaa atattaatca caaattatcg
    10201 ttaagacttg acctgattgg cactattgct tcggctgcct gtgccataca ttgtttcctg
    10261 atgccattta ttatcatcac actcacttat tatggaatga cattttttaa tgaaccatta
    10321 gtagaaatta tttttatttc agtaagtctg ctaattggaa tatttacttt tcgtcatgga
    10381 tatgtaaatc atcatcgaag tcttttgcct gcatgtttat ttatttccgg attaattatt
    10441 attgtaataa gtcattttct tttacacgaa catcaagaat ttcattatga tatggagagt
    10501 atattactct ttatttttac acctataggt gctgttatta tcgcttttag ccattactta
    10561 aacaggaaat tatccaaaaa caagcatgta ccttcatgcg attgctgaaa cattatatta
    10621 ataaaaaagt ggcttaaaat agttaagcca cttttaaatt tcaaaaacta attcatttaa
    10681 tttctttaaa ttgaattttt taaagcaaaa tatattctaa caaattttaa acggggatga
    10741 ttcaattttt taaattggta aatataatca tacttactta aatcttgtta ctttttctta
    10801 tatgaaattt tctttggcac agtaaactgt tttactcctt caacagaacc aagaaaccca
    10861 aaaccacttt gcttaattcc aacccacgga gtacctgaaa cacctcctat tccctgattt
    10921 acccctatca ttccggattc gatttgttct gctatctttc tacctttgtc tgtactttta
    10981 gtccaaacag ttgcgcctaa accaaataca gaatcatttg ctttttcgac agcttcttct
    11041 acatttttaa ctttctgcag acataccacc ggtccaaaag tctcattcat cattatatca
    11101 tgtttgtgat ttaaattgct gatcacagta ggttcaataa aaaatccgtc tccatctata
    11161 atacttccac caaataatat tttcgcacct tttttctttg cttcttcgat ctgaccaatt
    11221 acatgacttc tttgagcttc gctaaccata ggtcccatat cgacttcatc aaaaccatca
    11281 cctactttat atgttttaac tttttccagt acaagctttt caaattttcc aaatatactt
    11341 tgatcaacat aaattctttc tactgacaca caaacctgac ctgaatttct aagagatcca
    11401 tttactgcat aatttaccgc cttctctaaa tctgcatctt tcattactat catagggtct
    11461 tttccaccta attcaagcac aagtctgttt aacttttttg aacaagcttc cataattttc
    11521 ttaccaacag cctgcgatcc tacaaatgaa accatatcaa tgtctgttga caacagagct
    11581 tccccaactt cttcagctcc ctgcaggaga ttgatcactc ctttaggtaa gtatctgttg
    11641 aaaatattat aaattgcttt acctgttagt ggtgtatttt cagatggctt aaatacaact
    11701 gtatttccca tcaatatagc cggtgtaagt aatgattcag gcattccaac cggaaaattc
    11761 catggagtga ttactgcaac aacacctaat ggcattctat gaacttcagt aatgagcaga
    11821 ccttctttat gaatttctgt tactgcagct ttttctacta attttatatt ttcttcaatt
    11881 gcataggctg ttcctgctac ttcaccaact gccgatgaat aaacttttcc catctctttg
    11941 gtaataagtt ctccgatatg ttttttttct ttttcaagat cccttgctat ttttttaaat
    12001 aacaaaattc tttttttcaa tggagtttta ctccagactt taaaagcttt tctagctaac
    12061 tttactcctg atgcaatttc acccggcgaa gagcatttaa tcttttcaat taactcaccg
    12121 gttgaaggat taatatccat tataaatttt ctcatgaaat taaatatata tatttattta
    12181 taaaaacttt ttaaaaaaag ataaaacacc ttgcttccat gcaattctat gagtgactcc
    12241 agtgccttgc tgtatcttgt gtttattgtc cagataccac tgataagatc ttattaaagc
    12301 ttctgaattt gaatatttcg gattccattc caattgtttc atggctttat caattgagac
    12361 aaatgaatca gtatcagcag ttccatatat ccatttatat aatggggata catttaagat
    12421 ttcaaaaaat ctcaaaagag gtttaataag tgaagccgga gttttcataa ctttcgaacc
    12481 tgtgcctgca tagacacaca ttgcccctac atcttcattt acagttttaa attctttcgc
    12541 acctacatta aatgtatcat tgatcttgtc cagcggtaat gaagctgcct gtaagatagc
    12601 atcgacaagg tcttccactt caaaaagctg atatcggttt gtaccgtttc caataatagg
    12661 aatttttacg cctgattcga cccagtcata aagaatctga aacacgccta atcttgcagt
    12721 tccaataaaa cttttaggtc tcaagataca aatcggttga cctttttttc taatctcttc
    12781 acatacttct tctgcaagta ctttacttgt accatagggt ccgactcctt ctctctgatg
    12841 atcttcaaac aaaggatgta catcaggtac gccgtaaact gcagttgatg aaatatgtat
    12901 aactctgttc actttatttt tgagtgcact ttccaataca ttccttgtac catctacgtt
    12961 tgtctcaaaa atatctttct ttttccaaag tggtaatgcg gcagcacaat gaattataac
    13021 atcgacattt ttcatcattt catccatctg cgccttgtct cttacatctc cataaataca
    13081 tttaatcgcc ggatcatatt catctttctc aaactcagca atatcaaaaa atgtacagtc
    13141 tttaaaatca gttatccttt ttattttttg agaaatatga tatccaagaa atcctgcacc
    13201 tcctgttaca actattttca tttactttaa attgattaat taattaaaac gaattatata
    13261 gtttcaattc gctgtctctt agtgaaatat atttatcaga tataacatct gaaattatct
    13321 cggcaatgtc tccgggtttt gtccagcttt ttacatcttc agccgtcccc cattctctgt
    13381 ttgaaggtgt atcaatgatt ccggggatta tcgtattgca ccttatatta ttttttttat
    13441 actcttcacc taaaatattc atcaactgaa taaccccaag tttggaataa gaatatgcaa
    13501 atttttttgg agttaattca gtagcagatt tacttccaaa tgatattatt cttccgaaat
    13561 tattttttaa cataacattt aaaacttctc ttgagaaata aaatgcagac ataaaattca
    13621 gatcaaacat tttcataaaa gctggagttg aaagttcacc cgtattcaca ggactgtcaa
    13681 ttccgcctgc tgtatttata agaaaatcta ttttcccttt ttcctgaaca gcaacatttt
    13741 ttacaaactc aataacagaa gcttcatttg ttatatcaca ttcaaatgaa aatatttttt
    13801 ttaaattaaa atctactgag ttttcgtttt gagaattatc gaatactttg ttaaaagtgt
    13861 caagacttct ggaaggtata taaatcttaa tccctaaatg agctaatttt tctgagacaa
    13921 ctctccctaa tgcacctgta ccacccgtca ctattgctac tttgtcagtc atttaattta
    13981 agctatacaa attttcccta aaaaaacatt tttatctgcc cctgtcaccg aaattatatt
    14041 tgtcttattt ccggaaatta attcatttgc aagtactgca aaaagtacag cttctttatt
    14101 agatgaatta ataccgttta gattcaggat ctctaaatca gttccttcaa ataaatttct
    14161 gagattcttc atcagagtta gattgtttga tcctccgcca ctcacaaata ttttatcagt
    14221 tttaaatttc ttaatattat aattgactgc atatgctgta aaaaaattaa atgttgccat
    14281 cagatcataa gggtcatacg aattcttttt tacaatactt tcaataaatt cttcattata
    14341 gtattccctt cctgttgatt ttggagatgg ttttttaaca aatttatcct ttgtaacaat
    14401 cttttctaac aagtttgaat taatatttcc tttgccggct attttcccgt ctttatcaaa
    14461 ttttttattg aaatattttt tagagatcag atcaataagt aaatttccag gacctgtatc
    14521 aaatgctatc acttcattta atttacagtt cttttttata tatgtaatat ttgaaatgcc
    14581 gcctatattc agaaatactg aactgcagtt tatttttctg cttagaatgt aatccagata
    14641 aggaacaagc ggcgcaccat ctccatttac agaaacatct gctgttctga aatcaccaat
    14701 agttgtaacg cctgtaatat tggcaatcac agaaagatct cctatctgca ttgttgattt
    14761 ggaatcgtat ccgtattgat tattttttac cggataatga aatattgttt gcccgtggga
    14821 gcctgtcaaa tctatttcgt taggtgagat tttatttttt tttaaaaatt cattaatttt
    14881 aattccgaat aacctgccaa ttataaaatt taatttacaa atatcagcag tgtttccagt
    14941 cttttcggaa gaacaacgta atattaaatt ccttactttt ttatctatag gaaaagaatc
    15001 aaactccaat attctgattt ctgatttaat tcccgacccg taaatttcag ttaataccac
    15061 atcaactgca tcaattgaag tacctgataa aatcccgatt acttttctgc gggacttatt
    15121 tacaattcct tttaaaatgt tcattaatgt aacaaatata atttaattac gaattataat
    15181 ctatgttatt tggtatacaa aattaatatt taaaactaat tttcatattt aagacttaaa
    15241 gcaattttta gaaatactga acatattaaa taattcaaac aacataatta attatatctt
    15301 taataaatct gatggaatat aaattaaaat aataccattt attttaatta taattttatt
    15361 gattttggct caaattaaac tatatatata atggaagagt tttcaataaa aacaaagcag
    15421 catgataacg tttcaatttt agatcttgaa ggttatctcg atgctcacac ttctgttgaa
    15481 ttagagaagt gtttagagaa aataatttcg gaaaataaat tcaaaattat tgtaaatttt
    15541 gaaaaattat cctatatcag cagtgcagga ttgggagttt ttatggcatt tattgaaact
    15601 gcaagaaata atgccgggga tataaaactt tgtgctatga gtgacaaaat ttacaacata
    15661 tttgatatgt taggttttcc gatattgttt gaaatttata aagaagaaaa tagtgctctt
    15721 gaaaaattca gctgattgat ataaatggac aacacaaata aaatattgtt caaaagttca
    15781 acaaagaatt tatccttact aaggaaattt atagaggata aagcacttgc atttggattt
    15841 gatgaaagtt caataaatca gattatttta tcagttgatg aagcatgcac taacattata
    15901 aaacatgcac ataaatataa tgaaaaagag attattgaag tagatctaga ttcaaatgaa
    15961 aatcagttta taataaaact taattataaa ggaaaagcat tcgatccaaa taatatcagt
    16021 aacccggaca tgaatgatta ctttaataaa tttaaagtag gtggtctcgg agtacctatc
    16081 atgaaaaaat ttatgaataa aat
//
LOCUS       DAORVQ010000060        15936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1601_length_15936_cov_5.803224, whole genome shotgun sequence.
ACCESSION   DAORVQ010000060 DAORVQ010000000
VERSION     DAORVQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_15936_cov_5.803224"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLX80_06640"
     CDS             <1..385
                     /locus_tag="PLX80_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQY20506.1"
                     /translation="KKNLMLICGHYKGVDERVVKKLVTREISIGNYVLSCGDIAAFVF
                     IDAVARLIPGVLGDGESAITDSFQVETGFDHPQYTRPEMFNKMKVPEVLLSGDHKKIN
                     IWREKKANAKYNRINKLNKKNNRNG"
     gene            400..747
                     /gene="rplS"
                     /locus_tag="PLX80_06645"
                     /pseudo
     CDS             400..747
                     /gene="rplS"
                     /locus_tag="PLX80_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            910..1530
                     /locus_tag="PLX80_06650"
     CDS             910..1530
                     /locus_tag="PLX80_06650"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQY20507.1"
                     /translation="MNPKKKSFVSVAGNIGSGKSSLTSLIAGEFDWKPFYEKVNNNPY
                     LKDFYSDMNRWSFNLQVYFLSHRFKTHVEILKSRKSVIQDRSIYEDVEIFALNLYKMG
                     KMSKRDHDNYRQLFKEMTSFLKAPDLLLYLQSDLPKLQKQIHLRGRDFEKNIDPEYLK
                     KLNESYNEWISNYKSGRCVIVDSNDMDFVNRKGDLKKITELIKKSI"
     gene            1818..2903
                     /locus_tag="PLX80_06655"
     CDS             1818..2903
                     /locus_tag="PLX80_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20508.1"
                     /translation="MELDGEKTHLTGKKILIISLDALGNVLLNTSILRSLKRKYPVST
                     VHWITMKSALPILANNEFIDKVYVWDETDKMIISEMKYDVLLNGDKSDYACAFAGIVK
                     ADEKYGFVLNENGKIIPASESAMYNYSMGVNDELKFRKNLKSGSQIIHETFDLEYKEY
                     EYVFNFSKSETEFIEKYKEQIGYDANNLYIGFNTGCSNLFPNKKMTVEQHTSLINDLA
                     QTDGFKILLLGGKEDTERNLKIHESLDKEIQSKVIYTPTNLGIRNGACFMEVCDVVIT
                     GDSFGMHLAIALKKYVIAWFGLSCWNEIELFNRGEKLIPTGLECAPCWNKVCPYNLEC
                     IEMIDLQKIAGYVRKYALVKPSFTNKN"
     gene            complement(2959..3186)
                     /locus_tag="PLX80_06660"
     CDS             complement(2959..3186)
                     /locus_tag="PLX80_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20509.1"
                     /translation="METAIWVIGLLAAVIIIVLYIITSVNKYSNSFVEKYFDDDDKGK
                     ASGNDLTKAKDQNFPDEKVSLKDTDSKSGQN"
     gene            complement(3206..4327)
                     /locus_tag="PLX80_06665"
     CDS             complement(3206..4327)
                     /locus_tag="PLX80_06665"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016510495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQY20510.1"
                     /translation="MSNNKFPSFDLIESFGHEQVVFCSNKELGLKAIIGIHNTTLGPA
                     LGGTRMWNYASDEEALVDVLRLSRGMTYKAAVAGLNLGGGKAVIIGDSRTQKTEGLFR
                     AFGRYIEGLSGRYITAEDVGTSIKDMEYIMMETRHVTGIPIEVGGSGDPSPVTAYGTY
                     VGIKACINDIYGSDSLEGKKVIVQGAGGNVGKHLCKYLHEDGAVLYVNDIYEDKLKTL
                     VENYKATILNDNEIYSTEADVFSPAALGGIINDETIPLLKVKIVAGAANNQLGDEEKH
                     GKMLKDKGILYAPDYVINAGGLINVYNELEGYNRERALKQASGIYDILKSIIDISKKE
                     DVSTEVASRKIAEERMEAVSHAKRIRIYRDKRLFNQRNT"
     gene            complement(4359..5537)
                     /locus_tag="PLX80_06670"
     CDS             complement(4359..5537)
                     /locus_tag="PLX80_06670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20511.1"
                     /translation="MKRRAIFRIFDKYIGIPVIILLAILTKRKRRIPIENIKKILIIK
                     LAAIGDSILLIPTLRVLKKNFPDAEITFICSQINYSVIKKIPYVNKIIDCNVHSFLRK
                     PFSFFRFMKDLRKEKYDIIIDVGQWERINAIMTMLAKADYTIGFKTESQNKHFGYDSV
                     VAHSRTKHELENFLDLVIPLGINISEEDKKLEYFLNSEDYKFADEFWDKHELNGRTVI
                     CLHPGCGENGRPREWEEQNYIDLGRRLVAYDDNIRILITGAAHEEERCREIAAGINRN
                     VINTAGMYPLDNVVALVKRVKLIVCSNTGMLHIASCVGTKTMGLHGPTNPLKWGSYTK
                     NAVLIQSDKFCSPCLYLGHDYGCQVPQCMAHISVDDVFIYIRKALNPELFQNFQQHLN
                     "
     gene            5584..6030
                     /gene="cdd"
                     /locus_tag="PLX80_06675"
     CDS             5584..6030
                     /gene="cdd"
                     /locus_tag="PLX80_06675"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQY20512.1"
                     /translation="MNILTINKNTTILLTSYNKLIEYSINARKFSYSPYSKFSVGAAV
                     LTAEGKIFTGTNVECASYSLSICAERTAYSKAVSEGYKIFKAVAISSSAKDYTYPCGA
                     CRQFMAEFGTDVDIIIVKSKTKIKTYKLIDLLPGIFDKNFLLNQKK"
     gene            6027..6620
                     /locus_tag="PLX80_06680"
     CDS             6027..6620
                     /locus_tag="PLX80_06680"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQY20513.1"
                     /translation="MNENLQIRTFKKTGHIEVICGSMFSGKTEELIRRIRRAEIAKQR
                     VKVFKPKIDNRYSEFEIVSHNDQSYPSEVVENAEEILDKTFDVEVIGIDEAQFFENDL
                     IEICQNLADAGKRVIVAGLDQDYRAMPFEPMPQLLAIAEYISKTHAVCVICGAPANRT
                     HRVTQSDDQILVGASNHYEARCRIHHVFKTEPHKKAI"
     gene            6666..7691
                     /locus_tag="PLX80_06685"
     CDS             6666..7691
                     /locus_tag="PLX80_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY20514.1"
                     /translation="MNKFFTVICIVLFCLIMISCGKKESTSEKEIPLTPMKVGLVFDI
                     GGRGDKSFNDAAYKGLEEAKEKLGIEFEVIDPGDGSDRESALRKLASKKDIGLVFGVG
                     FIFTDDINNIALEFPDKKFACVDYSIDPDKKIPANVEAIEFKEEEGSFLVGVIAALNS
                     KTNKVGFIGGMESSLIRKFEEGYKQGVQYASPDCQVLTGYVSVSPEGFKNPGKAKEIA
                     LSQYNSGADIIYHASGLSGLGLFEAARDKNKLAIGVDMDQQKEAPGFILTSMVKQVDE
                     AVFNTIKNFKENKFIGGIKTFGLEDKGVSYVYDENNKSLIDTSVIAKVEDAKKKLANG
                     EITIILK"
     gene            complement(7702..14313)
                     /gene="sprA"
                     /locus_tag="PLX80_06690"
     CDS             complement(7702..14313)
                     /gene="sprA"
                     /locus_tag="PLX80_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQY20515.1"
                     /translation="MNFNSQKIIDSLKQPQKLVLIIIILFGISNVTFSQGRRDRTGVR
                     NNYVKVNPNDTSTVYSTEDSLSLIGVNQEPVDSTARIKYFQYEPVYKFGTKIQGRSSP
                     LLLGNSTLLKPEIKFDTSGNVILTENFNGEPIRAPLVIPLEEYLQALSANNIENLFSI
                     IVGENFKGGTQDDLGKLFEKFTDITIPLPFKSESIFGPPTFNLKLNGAVDITASYQSA
                     KSDQTVITGLSNSNNSINFKQEVQLTAKGVIGDKLSIDADYNTQRLFDFENQLKLKYE
                     GYSDEVIKKIEGGNVSLETRSGLIGSSQSLFGVKGEFQLGSLSLSSVVSQKTSKSETK
                     DYSGGVQESRYDINLWDYADNHFFLDTVYKTSFVDFYTTGSTISQRTDSLAVDEQSFE
                     VWVQTDQQTENYRRAAMYVDLGPIPSSGQYNDTLKSLRFNQQGVNASGIVRKLEESEY
                     ELHRLAGFVSLNVSLPDNYFVGVAYKRTKTREQFGTISTDSINAEDTLVLKMVKPENL
                     VPANTLAWEHKLKNIYKLPVSRVVKEGFEFEIKYLDNGNYIPTLPPSQNISRDLIFIV
                     GLDRYTNGRSGDPDNKFDFIPGTTINVESGYVIFPTLKPFLSNFEAYEENGVRIDSSF
                     WYPQIYTLSKNDSRLQPNANNFKITGKVRGEAGISNTISLGFNLVQGSVTIKVGELNL
                     VPNVDYTVDYSTGTVVIRNSSALISKDLKISYETNDLFTLASKTFLGLRGDYRISEKS
                     SFGFTFVNLKQQTLNDKVRIGEEPTNNSMFGIDFTTELPMQSFTNLVNLLPGFNTKEP
                     SSFSIRGEIASIIPDPNTKKSLIPGDNNEAVAYIDDMEGAKKIISLGTTYNTWTIGSV
                     PLSNYIGPADSIQYKQGKRGKLQWYNLPNSELVTNVYPYKDVQANQNTITPFYMTYVP
                     SQRGTYNYNGAYETTQDLDSTWNGVMKFLNTTSNDLINDNINFIEFSMRVVNLNAQTL
                     PGKLIIDLGNISQDAIPNNVLNTEDTLGNGELRSQADDKGLDFKTDDEELIEYNSKNG
                     TNLTLANFENGDPAGDNNNTNPVFNPNLINGTQGNLNFDNGRRPDTEDLNKTVRVNNG
                     NDYFQFEIDLDTSLTNKYIVGSGLNGWHQYKIPLSEFTTSYGNASFNNIQFVRMWVTG
                     IRDSVRLVMYEINLTGNQWVKPNKIDTTYNISTVSIEENPNFYQSPVPGNVLRQNIRN
                     QTGEETQSNEQSLSLEITNLTSGVKKVARKDYSTLPLDLFNYRTMKMFVNGDPSFNYT
                     NSDIYDATMVIRFGNDSNNYYEYRAPIHPDNRNPNRPEQPWDSLNNVTVNFNELTRLK
                     LLRDSANVPVTLPVPSIPGAEYRIFGNPDLKSIREITLGVEKNRNGLNSSISGSVWFN
                     ELRLVNVIDNDGVAFNVNANLKFADLMDFNFAISKTDPYFHSIDTRVGTRNTGLSWDF
                     GTTINVHKFINNALASMISEDWKDFITLPLTFRHSENTNNPQYFPGTDIEIDKAAEQQ
                     YLRVLSQTGDENQAQTARNNVYEEAQSLLVRNTLSINSMFFTLPGGNFFVKDFFNQLR
                     FNFNADFGNSRDITYENKNDFRYNGGVSFATDFKLSEKLNLNIGQLIPLGEKYANAKM
                     YFFLPFIPLVPLFSSNFSASTDFNRSRFESQQRKFSNPDPTSRDFTANRGFRLDWKFI
                     ENWIVDLNGNYDFRAGSDLTDYETGDTNGIQRPDSEIFNDIFFNGGVVNFGTDLDYVQ
                     TITLNPKLSLPFIDRFVTLTGGYNVRYGWVNPNQIVNVGFNTGYSNTFNSTANLKLAE
                     IFTLFGGSDNANLRANGMGRDTVEKSSQSIEDILNIFKTFVPMDISITYSQNSSVANG
                     GVTGNPGFGNFWFYPSTKDEYGPSRMYQLGFSLYPGTRAPNINSLTDTYLQGNELSLS
                     TSINPFIPENISMTLNFKTNWGFTNNLVYDSDANGNIGSPTSKSSSSLSGNSIFFAGN
                     MENFEFQGDGSNSTQSRLNLTNAFEEQVSSFPFPSWTLSISGLEKFPLFSDFASQVSL
                     ENNFLSEFRESRLVDINSLDIPNLQAVTQAFSPLIGLNIVFKELMGGNMSSNFRINSS
                     TSNILNPTGANVQSISTSEWSVNANFTKSGFSIPLFGLSLQNDISFALTLSNSKNEPT
                     NYEFGTGVKVPVIGNGSSITTINPSIQYSLSSKVSMQLFYKYISTNPTGQSVTTVPST
                     SNEGGLNIRITIQ"
     gene            14494..>15936
                     /locus_tag="PLX80_06695"
     CDS             14494..>15936
                     /locus_tag="PLX80_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HQY20516.1"
                     /translation="MFNIKVLNFSLFTVILLFLNSSFLYSQFDPHPELEWHTIETEHF
                     FVHYHDGTERTANTIAKIAEEIYGPITTLYKYTTPDKTHFIVNDVSDIANGATDYFGN
                     RIEIYATALDYDLRGTHNWLRNVVTHEYTHAIQVQASLKYGTKLPTFYLQWLGYENEV
                     RPDVLYGYPNAIVSYPISGVGVPAWFAEGTAQYQRQQLNYEYWDSHRDMILRSYIIGG
                     NLLTWGEMGQFSSITSLKAESIYNLGYSLTRYISEEYGEEKLREITDNLGDFTNFSMD
                     RAMKKALGTDGKKVYYQWKAFLENDYAKRLADVKADEVEGVVIENKGFANYFPQLSPD
                     GKKVAYLSNQEFDYGATSLMVYDIATRKSEIVNAPVSTNFSWSPDSKKILFAKRNYPR
                     TILGTTVYDIYELNVKSGDEKKLTENLRATSPAYAPDGKTMIFVTNRDGTLNLNLANT
                     DGKNIRPLTTFTNGEQVYNPKFSPDGKKIYF"
BASE COUNT         5187 a         2823 c         2775 g         5151 t
ORIGIN      
        1 aaaaaagaat ctaatgctga tatgcggtca ttataaagga gtcgatgaaa gggtcgtgaa
       61 aaagcttgta accagagaga tctcgatagg taattatgta ctgtcctgcg gtgatattgc
      121 tgcatttgtt tttatagatg ccgttgcaag attgataccc ggagttctcg gagacggtga
      181 atctgcaatt acagattcat ttcaggtaga gacaggattt gatcatccgc aatatacaag
      241 acctgagatg ttcaataaaa tgaaagtacc tgaagtatta ctttcaggag accataaaaa
      301 aataaatatc tggcgtgaga aaaaagccaa tgcaaagtat aacagaataa ataaacttaa
      361 taagaaaaat aacagaaacg gataaggaga attattatca tgaataaaaa aattaatcta
      421 gtaacagcag gacagatccg gaatgattta ccggagtttc acgtaggaga tacaattact
      481 gtatcagtaa aagtatttga aggtgaaaag gaaaggattc agctattcaa aggaatagta
      541 atgggaataa gaggcggtgg tgtcagcaag aatttccgcg tcaggaaaat ttccaatgga
      601 gtaggagtgg aaagaatttt cccaatcaac tcaccaagaa tagctaagat cgaaatttcc
      661 aaatacggaa gtgtaagaag agctaaactt tattatttga gaaatctgac aggtaaggct
      721 gctacaaaaa taaaagaaaa aaagaaagct ccaaaagttt ctgagcagat aattcctgaa
      781 gcaccggtat tatctaaaaa agctgaaacg gttgaagtaa tagaagaagc agtagtttct
      841 tagaatttaa ttcttttaaa ataaaatatt ttttaaccgt caattttaat ttgacggttt
      901 ttttatttaa tgaatccgaa aaaaaaatca tttgtatccg tagccggtaa tataggttcg
      961 ggtaagtcat ctcttacaag tctgatagcc ggagaatttg actggaaacc tttttatgaa
     1021 aaggttaaca acaatccata tcttaaagac ttctattcgg atatgaacag gtggtcattc
     1081 aatctgcagg tatatttcct ttctcacaga tttaagacac atgtcgaaat tctaaaatcc
     1141 agaaagtctg taatacagga caggagtatc tatgaggatg ttgaaatttt tgcacttaat
     1201 ctgtataaga tgggaaaaat gagtaaacga gatcatgata attacagaca gctctttaaa
     1261 gaaatgactt ctttccttaa agcacctgac ctccttttat atctccagtc cgatctgccg
     1321 aagttacaaa aacagattca cctaagggga agggattttg aaaaaaacat cgaccctgaa
     1381 tatctgaaaa agcttaatga aagctataat gaatggatca gtaattacaa atcaggcaga
     1441 tgtgtaattg ttgattcaaa tgatatggat tttgtaaaca gaaaagggga tctgaaaaag
     1501 atcactgaac ttataaaaaa atcgatttaa atttttttaa cgcagtatcc tcagagtccg
     1561 aaaaacacgt gactcttagg gaacaaaaga tcagcaatat tattttcaag acttatttga
     1621 aacggagttt caagttcaag tgcgttacca atccggagat taggaacggg cactaaaaaa
     1681 tcagaattaa atttatttat tgaattaatt tgtatttata attggacttt aaaaatctaa
     1741 aattaccgga ctgtaatcat ttctcaggct ataagccatg cttccccggc catgactgtc
     1801 tgacacaagg ctgtaaaatg gaacttgacg gagaaaaaac acatctcacc ggaaaaaaaa
     1861 ttcttatcat atcacttgat gctctcggaa atgtgctatt gaatacatcc attcttagat
     1921 ctttaaaaag gaaatatcct gttagcactg tccactggat cactatgaag agtgcattac
     1981 ctatacttgc aaataatgaa tttatagata aagtatatgt atgggacgaa actgataaaa
     2041 tgataatttc tgaaatgaag tatgatgttt tactgaacgg agataaatcg gattacgcat
     2101 gtgccttcgc cggcattgta aaagctgatg agaaatacgg attcgtttta aatgagaacg
     2161 gaaagatcat tcccgcaagt gaatccgcaa tgtataatta cagcatgggg gtcaatgatg
     2221 agttaaaatt cagaaagaat ctgaaatcag gaagtcagat aatacatgaa acttttgatc
     2281 tggaatataa agaatatgag tatgtattta atttttcaaa gagtgaaact gagtttatag
     2341 aaaaatataa agaacagatc ggatatgacg ctaacaattt gtatattgga tttaataccg
     2401 gctgttcaaa tctttttcca aataagaaaa tgactgtaga gcagcatact tctttaatta
     2461 atgatcttgc tcagacagat ggatttaaga tattgctgct tggcggaaaa gaggataccg
     2521 aaaggaatct gaaaattcat gaaagtctcg acaaggaaat tcagagcaaa gtaatttata
     2581 cacctaccaa tcttggcatc agaaatggcg cgtgttttat ggaagtctgt gatgtagtaa
     2641 ttacaggcga tagttttgga atgcatcttg caattgcatt gaaaaaatat gtgatagcat
     2701 ggtttgggct ttcctgctgg aatgagatcg aactgtttaa ccgcggggag aaattaattc
     2761 caaccggtct ggagtgtgct ccatgctgga ataaagtttg tccttacaat ttggaatgta
     2821 ttgaaatgat tgaccttcaa aaaattgccg gatatgttag aaaatatgca ctggtgaagc
     2881 cgtcatttac aaataaaaat taagtccgct tactcccctg cttaaaataa tattaaaatt
     2941 ctttaagata aattgatctc aattttgacc ggactttgaa tcagtatctt ttaaactaac
     3001 tttctcatcg ggaaagtttt gatctttagc ttttgttaaa tcatttcccg aagcttttcc
     3061 tttgtcatca tcatcaaaat atttttcgac aaatgagtta ctatatttat ttacagaagt
     3121 aattatatat aaaacaataa ttattactgc tgcgagaagg cctattaccc agatagcagt
     3181 ttccaaattt tattccaatt tattattatg tattacgctg gttgaaaagt cttttatctc
     3241 tgtaaatcct gattctcttg gcatgagaaa cagcttccat tctttcttca gcaatttttc
     3301 tcgaagccac ttctgttgat acgtcttctt ttttagagat atcaattata cttttaagaa
     3361 tatcgtaaat tcctgaagcc tgctttaaag ctctttctct gttatatcct tcaagttcat
     3421 tataaacatt tataagtccg cctgcattta tcacataatc gggtgcataa agaatacctt
     3481 tatccttgag cattttgccg tgtttttctt catcaccgag ctgattattt gcagcacctg
     3541 ctacgatttt gactttcagc aatggaattg tttcatcatt aataatacct ccgagagcag
     3601 cgggagaaaa gacatcagct tcagtagaat aaatttcatt atcatttaaa atagtggctt
     3661 tataattttc tacaagtgtt ttcagcttgt cctcatagat atcattaaca taaagaactg
     3721 ctccgtcttc atgcagatat ttacagagat gtttaccaac gttaccgccg gcaccctgta
     3781 caattacttt tttcccttca agagaatcac ttccatatat atcattaata catgctttta
     3841 taccaacgta tgttccataa gcagtaacag gagacgggtc tccactaccg cctacttcaa
     3901 taggaatacc tgtaacatgc cgtgtttcca tcataatata ttccatgtct ttaatgctcg
     3961 ttcccacatc ttcagcagtt atatatcttc cggaaagtcc ttcaatatat ctgccgaaag
     4021 ctctgaagag accctcagtt ttttgagttc ttgaatcacc aataatcaca gcttttccgc
     4081 cgccgagatt taatccggct acagcagctt tataggtcat tcctctggat aatcttaata
     4141 catctaccaa agcttcttca tccgaagcgt aattccacat cctcgttcca ccaagggctg
     4201 gtcctaaagt tgtattatga attccgatta ttgctttcaa accaagctct ttattggaac
     4261 agaaaactac ttgctcatga ccaaatgatt caatcagatc aaatgaagga aatttattat
     4321 ttgacatatt tttagtttaa taattaataa tttttttgtc aatttaagtg ttgctgaaaa
     4381 ttttggaaca attcaggatt caaagctttc ctgatataaa taaaaacatc atctaccgaa
     4441 atatgcgcca tgcactgcgg aacctgacat ccatagtcat gaccaagata aagacagggg
     4501 ctgcaaaatt tatcactctg gatcagaact gcattcttgg tgtaagatcc ccattttaaa
     4561 ggatttgtcg gaccgtgcag tcccatagtt ttagttccga cacaggaggc aatatgaagc
     4621 atacctgtat tactgcatac aattaattta actcttttga caagtgcaac tacgttatca
     4681 agcggataca ttcctgcagt gttaataaca tttctattta taccggcagc aatttctctg
     4741 catctttctt cttcgtgagc agcaccggta ataagtatcc ttatattatc atcataagct
     4801 accagccttc ttcccaggtc aatataattc tgttcctccc attcccgggg tctgccattt
     4861 tctccgcaac caggatgaag acaaataaca gttcttccgt tcagctcatg cttatcccaa
     4921 aattcatctg caaatttata atcttctgaa tttaaaaaat attctaattt tttatcctct
     4981 tcagaaatat taataccaag aggaatgact aagtcaagga aattctcaag ttcatgcttt
     5041 gtcctggaat gagctactac actgtcatat ccaaaatgct tattctgtga ttcagtctta
     5101 aatccaattg tgtaatctgc tttggcaagc attgtcatga ttgcattaat tctttcccat
     5161 tgacctacat caataataat gtcgtacttt tctttcctca gatctttcat gaacctgaaa
     5221 aatgaaaatg gttttcttaa aaatgaatgt acattgcagt caataatttt attaacatat
     5281 ggaatttttt ttataactga ataattgatc tgagaacaga taaatgtaat ttcagcatca
     5341 gggaaattct tttttaatac tcttagagtt gggattaaca gtatagaatc accaatggca
     5401 gcaagcttaa ttatcaatat ttttttaata ttttcaattg gaatgcgcct cttccttttt
     5461 gtaagtattg ccagcaaaat aataacagga ataccaatgt atttatcgaa tatcctgaat
     5521 atggctcttc gtttcacagt gtaagtatct gaagaataaa attatttgat aatatactta
     5581 gaaatgaata tattaacaat aaataagaat accactatat tgctgacatc atataataag
     5641 ttaatagaat attccattaa tgcaagaaaa ttttcatatt caccctattc caaattttcg
     5701 gtaggtgcag cagtactaac cgctgaaggc aagattttta cagggacaaa tgtagaatgt
     5761 gcatcttact ctttgagtat ctgtgccgaa cgaacagctt actcgaaagc tgtatccgaa
     5821 ggatataaaa tttttaaggc tgttgctatt tcatcaagtg caaaagacta tacatatccg
     5881 tgcggagcct gccggcaatt catggcagaa ttcggtactg atgtggatat tataattgtg
     5941 aaatccaaaa ccaagataaa aacttataag ctgattgatc tgctgcccgg tatctttgat
     6001 aaaaattttt tactgaatca aaaaaaatga acgaaaattt acaaatcaga actttcaaaa
     6061 aaacaggaca tattgaagta atttgcggca gtatgttttc aggtaagaca gaagagctca
     6121 taagaaggat aagaagagct gagatcgcta agcagagggt caaagttttc aagccaaaaa
     6181 ttgacaaccg atacagtgaa tttgagatcg tgtctcataa tgatcagtct tatccgtcgg
     6241 aagtagtgga aaatgctgaa gagattcttg acaaaacttt tgatgtggaa gtcattggaa
     6301 tagacgaagc tcagtttttt gaaaatgact taattgagat ctgtcagaat cttgcagatg
     6361 cgggaaaaag ggtcattgta gccggtctgg atcaggatta cagggcaatg ccttttgaac
     6421 caatgcctca gcttctcgcc attgcggaat atatttcaaa aacacatgct gtctgtgtaa
     6481 tttgcggagc acctgcaaac agaactcaca gagttactca aagtgatgat cagatactgg
     6541 tcggcgcttc aaatcattac gaagccagat gccgtattca tcatgttttt aaaactgaac
     6601 ctcataaaaa agcaatctga atatattcac acttcatact aaccttttat aataaaaaaa
     6661 tataaatgaa taaatttttc acagtaatat gtattgttct gttttgtctg attatgattt
     6721 cctgtggtaa aaaggaaagt acttcggaaa aagaaattcc gcttactcct atgaaagtcg
     6781 gattagtatt tgatattggc ggcagggggg ataaatcttt taatgacgcc gcttataaag
     6841 gtcttgagga agcaaaagaa aaactcggga ttgaatttga agtgatagat ccgggagacg
     6901 gctctgacag agaatctgct ttaagaaaac tcgccagcaa aaaagatatt ggactggttt
     6961 ttggggtagg gtttattttt acagatgaca taaataatat tgcattggaa ttccctgata
     7021 aaaaatttgc atgtgtagac tattcaattg atccggataa aaagatcccc gcaaatgtag
     7081 aagctattga atttaaagaa gaggaaggtt cttttctggt tggtgtaata gcagctttaa
     7141 attccaaaac aaataaagtc ggatttatcg gaggcatgga atcaagtctc ataagaaaat
     7201 ttgaagaagg atataaacaa ggagtacaat atgcctctcc tgattgtcag gttttaaccg
     7261 gctatgtaag cgtttccccg gaaggattta aaaaccccgg gaaagcaaaa gagatcgctt
     7321 tgagtcagta taatagtggt gctgatatta tttatcacgc atcgggattg tcggggctcg
     7381 gactttttga agctgccaga gataaaaata aactagctat aggcgtagat atggatcagc
     7441 aaaaagaagc gccgggattt attcttacaa gcatggtaaa acaagtggac gaagcagttt
     7501 tcaatactat aaagaatttc aaagaaaata aatttatcgg cggcataaaa acttttggtc
     7561 tcgaagacaa aggtgtaagt tatgtttatg atgaaaataa caaatctctg atcgatactt
     7621 ctgtgatagc aaaagtggaa gatgcaaaaa agaaattagc taatggtgaa attacaatta
     7681 tattgaaata aagaaaattt cttactggat agtgatcctg atattaagac cgccttcatt
     7741 tgaagtactt ggaacagtag ttacagactg accggtagga ttagttgata tatatttata
     7801 gaagagctgc atagaaacct ttgagctgag ggaatactga attgaaggat ttatagttgt
     7861 gatggatgaa ccgtttccta tgacaggtac ttttactccc gttccaaatt cataatttgt
     7921 aggctcattc tttgaattcg aaagtgtcag tgcaaatgaa atatcattct gaagtgacaa
     7981 tccaaagagc gggatactga acccgctttt tgtaaagttt gcatttacac tccattcact
     8041 tgtacttatt gactgaacgt ttgcaccggt aggattcaga atatttgacg tactggaatt
     8101 tatcctgaaa tttgaggaca tatttccacc cattaattct ttgaaaacaa tattcagccc
     8161 tatcagcgga ctaaatgcct gagttaccgc ctgtaaattt ggaatatcaa gactattaat
     8221 atctactaat ctgctttccc tgaattccga aagaaaatta ttttccaatg aaacctgtga
     8281 agcaaaatct gaaaacaaag ggaatttttc aagacctgaa atactcagag tccagcttgg
     8341 gaaaggaaat gaagatacct gttcttcaaa agcatttgtc aaattcaatc tgctttgagt
     8401 gctgttcgaa ccatctccct gaaattcaaa attttccata tttcctgcaa agaatataga
     8461 attgcctgaa agacttgagc tcgacttact tgtaggagaa ccaatattac cgttggcatc
     8521 agaatcatac acaagattat tagtaaatcc ccagttcgtt ttaaaattca gcgtcatact
     8581 gatattttca ggaataaatg gatttatcga agtagataat gaaagttcat ttccctgcag
     8641 ataagtatca gttaacgagt taatattagg agctctggtt ccaggataca gactgaatcc
     8701 aagctgatac attcttgacg gaccatattc atccttggta gatggataaa accagaagtt
     8761 tccaaatccc ggatttccag ttacgcctcc gtttgccact gaactattct gagaatatgt
     8821 tatactaata tccattggta caaaagtttt aaaaatattt aaaatatctt caatgctttg
     8881 cgaacttttt tcaacagtat ctcttcccat tccatttgct ctgagattag cattatctga
     8941 cccgccaaac agagtaaata tttctgctaa ttttagattt gctgtagagt tgaaagtatt
     9001 tgaatatccc gtattaaaac ctacatttac tatctgattt ggatttaccc agccatatcg
     9061 gacattatat ccgcctgtca aggtaacaaa tctgtcaatg aaaggcaggc tcagtttggg
     9121 atttaaagta attgtctgta cataatccag atcagttcca aaattaacaa cacctccatt
     9181 aaaaaatata tcattaaata tttcgctgtc gggtctctga ataccgttag tgtcgccagt
     9241 ttcgtaatcg gtaagatcac ttccagcccg gaaatcgtaa tttccattca ggtcaacgat
     9301 ccagttttca atgaatttcc agtcaagccg gaatcctctg tttgctgtaa aatctctgga
     9361 tgtcggatca ggatttgaaa attttctttg ctgagattca aatctcgatc tgttaaaatc
     9421 tgttgatgca gaaaaatttg aactaaaaag gggaaccagc gggatgaacg gcagaaagaa
     9481 atacatcttc gcatttgcat acttctcacc taaggggatc agttgaccta tattcagatt
     9541 taatttttct gaaagtttaa aatcagttgc aaagctgact ccaccgttat atctgaaatc
     9601 atttttattt tcataagtaa tatctcttga gtttccaaaa tcagcattaa aattaaatcg
     9661 aagctgatta aagaaatcct tcacaaaaaa attaccaccc ggtaatgtaa agaacatact
     9721 attgattgaa agtgtatttc taactaataa actctgtgct tcttcatata cattatttct
     9781 tgcagtttgt gcctgatttt catcccctgt ctgagataag acccgcaaat actgctgttc
     9841 tgctgcttta tcaatttcaa tatccgtacc cggaaaatac tgagggttat ttgtattttc
     9901 agaatgtctg aaagtaagcg gaagagttat aaaatccttc cagtcttcgg agatcataga
     9961 cgccaaagcg ttattaatga atttatgtac attgatcgta gttccaaaat cccatgataa
    10021 acccgtattt cgggtcccca cccttgtatc aatagaatga aagtatggat cggtctttga
    10081 tattgcaaaa ttgaaatcca tcaggtcggc aaatttcaga tttgcattta cattaaaagc
    10141 tactccgtca ttatcgatta cgtttacaag ccgcagttca ttaaaccata cacttcccga
    10201 aatggaagaa ttcagtccat tcctgttttt ttccacacca agtgtaattt ccctgataga
    10261 tttcaaatca ggattaccaa agattctgta ttctgcacca ggtatactcg gtaccggcaa
    10321 cgtaacaggt acattagcgc tgtctctgag caatttcagt ctggtcagtt cgttaaaatt
    10381 aactgtaaca ttgttcaaag aatcccacgg ctgctcagga cggtttggat ttctgttatc
    10441 aggatgtata ggtgctctgt attcgtaata attatttgaa tcatttccaa atcgtattac
    10501 cattgttgca tcatagatat ctgaatttgt ataattaaat gacgggtctc cgtttacaaa
    10561 cattttcatt gttctgtaat taaaaagatc cagtggtaat gtactgtaat cttttcttgc
    10621 aacttttttt acaccgcttg taaggtttgt aatttcaagt gataaagact gttcgtttga
    10681 ctgtgtttct tcaccggtct gatttctgat attctgtctc agcacattac ccggaacagg
    10741 actctgataa aaatttggat tttcttcaat gctgactgtg gagatattat aagttgtatc
    10801 tattttattt ggttttaccc actgatttcc tgtcaggtta atttcataca tcacaagtct
    10861 gactgaatct ctgataccgg taacccacat tctgacaaac tgaatattat taaaagaagc
    10921 atttccgtat gatgttgtaa actcagataa tggtatttta tactgatgcc atccattcaa
    10981 tcctgaacct actatatatt tattggtaag tgaagtatcc agatctattt caaactggaa
    11041 ataatcatta ccgttattta cccggacggt tttattcaaa tcctccgtat ccggtcttcg
    11101 tccgttatca aaattcagat ttccctgtgt accgttaatt aagtttggat tgaatactgg
    11161 attcgtatta ttattatcgc cggcaggatc tccattttca aaatttgcca gagttaagtt
    11221 agtaccattt ttactattat attcgattaa ttcttcatca tcagttttaa agtcaagacc
    11281 cttatcatcc gcctgagatc tcagttcacc attaccaagt gtatcttcag tatttagaac
    11341 gttgttagga atagcatcct gacttatatt tcccagatca ataattaatt tcccgggcag
    11401 tgtctgagca ttcagattca cgacacgcat actgaattca atgaaattta tattatcatt
    11461 gataagatca ttacttgttg tattcaaaaa tttcattact ccgttccatg tagaatcaag
    11521 atcctgagtt gtttcataag cgccattata attatatgtt cctctttgac ttggaacata
    11581 agtcatataa aaaggcgtga tcgtattttg atttgcctga acatctttat atggatatac
    11641 atttgtaaca agctcactgt ttggaaggtt gtaccattgc agttttcctc ttttaccctg
    11701 cttatactga atactgtctg caggtccgat ataatttgaa agaggtacag atccaattgt
    11761 ccaggtattg taagtagtac caagtgaaat tatttttttt gcaccttcca tatcatcaat
    11821 ataagcaact gcttcattgt tatcgcccgg tataagactt ttctttgtat ttggatcagg
    11881 gataatggat gcaatttcac ctcttattga aaaactggat ggctcttttg tattaaatcc
    11941 gggaagcaga tttacaaggt ttgtaaagga ctgcattggc agctctgtag taaaatcaat
    12001 tccgaacatt gaattatttg tcggctcttc acctatcctt actttgtcgt tcaatgtctg
    12061 ctgcttcaga tttacaaatg taaaaccgaa tgaacttttt tcggagatcc tgtaatcacc
    12121 tcttaatcca agaaatgttt ttgaagccag cgtaaaaagg tcatttgttt catatgagat
    12181 cttaaggtct tttgatatta atgcggatga atttctgatc acaactgtac cagtagaata
    12241 atcaactgta taatccacat taggtacaag attaagttca ccgactttta ttgtaacact
    12301 tccctgtacc agattaaatc cgagactgat tgtgttactg ataccggctt ctcctctgac
    12361 tttaccagtt atcttaaaat tgtttgcatt tggctgtagt ctggaatcat tctttgataa
    12421 agtatagatc tgtggatacc aaaagctgga atcaatcctg actccgtttt cttcataagc
    12481 ctcaaagttt gataagaaag gttttaaagt cggaaaaata acataaccac tttctacatt
    12541 aatggtagta ccgggtataa agtcaaattt attgtcggga tcacctgacc ttccgtttgt
    12601 atatctgtca agtcctacta taaagatcaa atctcttgaa atattctgag acggaggaag
    12661 agtcggtata taatttccat tatcaagata ttttatttca aattcaaatc cttcttttac
    12721 aactctcgat acaggcagtt tatatatatt ttttaatttg tgttcccacg caagagtatt
    12781 tgccggaaca aggttttcgg gctttaccat tttcaggacg agtgtatctt cagcatttat
    12841 ggaatctgta ctaatagttc caaactgttc acgggttttg gttcttttat atgctacacc
    12901 tacaaaataa ttgtctggta aacttacatt caaagaaaca aaacctgcta atctgtgaag
    12961 ttcatattca ctttcctcta actttctgac aatacctgac gcattaactc cctgctgatt
    13021 gaaccttagg gattttaaag tatcattgta ctgaccgctg gaaggaattg gtccgagatc
    13081 cacatacatt gctgctcttc tgtagttttc tgtctgctga tccgtctgta cccagacctc
    13141 aaaagactgt tcatccacgg caaggctgtc agttctttga cttatagtac tgcctgttgt
    13201 ataaaaatct acaaaactgg ttttataaac tgtatctaag aaaaaatgat tgtcagcata
    13261 atcccataaa ttaatatcat atctcgactc ctgcacacct ccgctgtaat cctttgtttc
    13321 tgatttactg gttttctgtg aaactacgct agacaaagat aatgacccga gctgaaattc
    13381 acctttgaca ccaaacaagg actgagatga accgatcagg ccggatctcg tctcaagaga
    13441 aacatttcct ccctctatct ttttaataac ttcatcagaa tatccttcat atttcagctt
    13501 gagctgattt tcaaaatcaa ataatctctg tgtattataa tctgcatcaa tgcttagttt
    13561 atctccgatg acaccttttg cagtaagctg aacttcctgt ttaaagttaa tactgttatt
    13621 tgaattactc aaaccggtta ttacagtctg atcacttttt gcactttgat aagaagctgt
    13681 aatatctaca gcaccgttta gttttaaatt aaatgtaggg ggaccgaata tactttctga
    13741 tttaaatgga agtgggatag ttatatctgt aaatttttca aagagttttc caagatcatc
    13801 ctgagtaccg cctttgaaat tttcgccaac aataattgag aataaatttt caatattatt
    13861 tgccgacagc gcttgtaaat attcctcaag gggaattacc aatggagctc ttattggttc
    13921 gccattaaaa ttttcagtaa gtattacatt tcctgatgtg tcaaatttta tttcgggttt
    13981 taacaatgta gaattaccta ataataatgg tgaagatctt ccctgaattt ttgtcccgaa
    14041 tttataaaca ggttcgtatt ggaaatattt gattcgggct gtagaatcca caggctcctg
    14101 gtttacaccg atcagactaa gtgaatcttc tgtcgaataa acagtgctgg tatcatttgg
    14161 attgaccttt acataattgt ttcttacgcc tgtcctgtct cttcttcctt gagaaaaagt
    14221 aacattgctt ataccaaata aaattattat tattaaaact aatttttgag gttgttttag
    14281 cgaatcaatt attttctgtg aattaaaatt caactacaag agttatttat tacaaaattg
    14341 taaaattctt ctataagctt tattttaacc ttttaaaaat ttattattta tttgataatt
    14401 tcttaccgat tctagaattc tttgcagttt caaatcttta tttaaaatca aatttatttt
    14461 taacaattat aattattaac aaattaacat tcaatgttta acattaaagt cctgaatttt
    14521 tctctattta cagttatcct tttatttcta aactcatctt ttctgtattc gcagtttgat
    14581 ccgcatcccg aactcgaatg gcatactatc gagaccgaac atttttttgt tcactatcat
    14641 gatggtacgg aaagaactgc aaatacaatt gcaaaaatcg ctgaagaaat ttatggtccg
    14701 atcactacac tgtataaata taccacacct gataaaactc atttcattgt taatgacgta
    14761 tccgatattg caaacggagc tacagattat tttggcaaca ggatagaaat ttatgcaact
    14821 gctcttgact acgatctcag aggtacacac aactggctga gaaatgttgt cactcacgaa
    14881 tatactcatg ctattcaggt tcaggcatct ttaaaatatg gaactaaact tccaacattt
    14941 tatctgcagt ggctcggtta tgaaaatgag gtcagacccg atgttttata tggttatcca
    15001 aatgccatcg tctcttaccc gatttcgggg gtcggagttc ccgcatggtt tgcagaaggt
    15061 actgcgcagt atcagagaca acaattaaat tatgaatact gggattcaca cagagatatg
    15121 attttaagaa gctatattat cggaggtaat cttcttacct ggggtgagat gggacaattt
    15181 tcttccatca ccagtcttaa agcagaatcg atatataatc tcggatattc tctcacaaga
    15241 tatatttcag aagagtacgg tgaagaaaag ctcagagaga tcacagataa cttaggagat
    15301 tttacaaatt tcagtatgga cagagctatg aaaaaagctc tgggcactga tgggaaaaaa
    15361 gtatattatc agtggaaagc atttctcgaa aatgattatg caaaaagact tgcagatgta
    15421 aaagctgatg aagtcgaagg tgtcgttatt gaaaataaag gttttgcaaa ttattttccg
    15481 cagctctcac ctgacggaaa aaaagtagct tatttatcaa atcaggaatt tgactatgga
    15541 gcaacaagtc tgatggtgta tgacattgca acaagaaaat ctgaaatagt aaatgcacca
    15601 gtctcaacca atttctcctg gtcacctgat tctaagaaaa ttcttttcgc aaaaagaaat
    15661 tacccgagga cgattctcgg gaccactgtt tatgacatat atgagcttaa tgtaaaaagt
    15721 ggtgatgaaa aaaaacttac tgaaaatcta agagctacat ctcctgcata tgctcctgac
    15781 ggtaagacaa tgatttttgt tactaacaga gacggtactt tgaatcttaa tttagcaaat
    15841 acagacggga aaaatatcag acctctgact acctttacaa acggcgaaca ggtctataac
    15901 cctaaatttt ctcctgacgg aaaaaaaata tatttt
//
LOCUS       DAORVQ010000061        15835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1613_length_15835_cov_5.001965, whole genome shotgun sequence.
ACCESSION   DAORVQ010000061 DAORVQ010000000
VERSION     DAORVQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15835
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1613_length_15835_cov_5.001965"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..551
                     /locus_tag="PLX80_06700"
     CDS             393..551
                     /locus_tag="PLX80_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20517.1"
                     /translation="MKTNSITAFIWDIKNINNELKGLFDSAKDPFDPMLNDLVNRKVH
                     LLFFGFKF"
     gene            824..1489
                     /locus_tag="PLX80_06705"
     CDS             824..1489
                     /locus_tag="PLX80_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQY20518.1"
                     /translation="MKSHRSVISVFIIFFIAAFTLQKESTAQTKLGITTGFTLSTISG
                     DAPDNSEYSSSSGLVLGLTGEFNITKDLKINLQPQYFSNNSIISYDLNRKEPIDSLKL
                     NFQYISLPIILKVPALSSITYFNSGFSISYLMNSKITDIDGADYNVDLNDLVNKFNLA
                     ILFGVGLNFPVYKQFELEFEARYTQSILNLSNGSTSFVGTLPDRYRSSGFMILADIIY
                     SFK"
     gene            1555..2487
                     /locus_tag="PLX80_06710"
     CDS             1555..2487
                     /locus_tag="PLX80_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20519.1"
                     /translation="MKAQNNSDVKISDRDLPEITKPNLNGHNFIITSSFKGPFISTSI
                     KTLLGAGQTLGLELPAVRLDTFTLNRDIGSILITSLDVEYEAQIREWLSFYLSYQIYG
                     RLGTKTTTLVAQGVNLASGYQIGMMFKLWEKKNMLLSGTVNVSNKSYTIIDLENYISG
                     IIENGFDENDLLVYSIPSLSVLGGANYAWAIDRSWGLVATLELGYGESINKSSNDNFL
                     INTGVSVDFDLLPRSKVPIGFNLGYYNNSFPALSGDRIGNPQNFFGQIAYTGRNDLAA
                     GFELSYQFSKPESYNTDGKVTKFLTIYGNLKYYF"
     gene            2637..2710
                     /locus_tag="PLX80_06715"
     tRNA            2637..2710
                     /locus_tag="PLX80_06715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2671..2673,aa:Arg,seq:cct)
     gene            2750..3484
                     /locus_tag="PLX80_06720"
     CDS             2750..3484
                     /locus_tag="PLX80_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY20520.1"
                     /translation="MNSSNKVALVTGGARRLGKHISLSLAESGYDIVLNYNESGKLIL
                     EKILKNLSEFNVNVTAVKCDLTKVKEIKKMFSVVSLKYGKLDLLVNNAAVFKKSDFFE
                     TTEKMYDNFLDTNLKGMFFCCQEAAKIMLKSENKISRIINIASLGAIENWTGYIPYSL
                     AKTGVIKLTSQLGKKLAPEIIVNAIAPGTIEMEDEVNADKENINKYPMKKFADTGDIL
                     SLVKYLALENKYITGQTFIVDGGKSL"
     gene            3536..4375
                     /locus_tag="PLX80_06725"
     CDS             3536..4375
                     /locus_tag="PLX80_06725"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HQY20521.1"
                     /translation="MKFLNKSPKAYHDLDFLNSPQARDIRILTEYKEPLERFKKHKIY
                     NTVVFFGSARTLPRKTALKNLKEINNIINGNENVSLTLQKQLKSAETDLLMSRYYEET
                     VELAEMMTKWAMSLGKPHKYVVCSGGGPGIMEAANKGATKAKGLSIGLNISLPFEQYP
                     NPYITPDLNFEFHYFFMRKFWFAYLAKALVIMPGGFGTLDELMEILTLIQTEKLRKKF
                     TVVVYGTEFWNKIINMPMMAELNVISPEDLKLFKFVDSPQEAFEFLTKELLKNYINKK
                     FKK"
     gene            4372..5253
                     /locus_tag="PLX80_06730"
     CDS             4372..5253
                     /locus_tag="PLX80_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQY20522.1"
                     /translation="MIISMTGFGKSEGSYKGRKYYVEIRSVNNRYSDLSLRYPRQFAS
                     YDFELKEIVKKKISRGKISVNITAESQETAIGNLNLDSVLIKKYAEELNKINSLIGSD
                     EKVRLEHILRLAEMSSPEVSTESDESEKIFLFKLIDKAVEDLNNMKKKEGDALKKDII
                     ERINFIKKDSEIITKLSSSKTSVLRKKLKDKINNLLSDNKLIDESRLETEIILYAERI
                     DITEELIRLKSHIKYFTDNIKSSELSGRRLNFLTQEINREVNTISSKSMDAEISQRGA
                     VMKEELEKIREQLQNVE"
     gene            5263..5820
                     /gene="gmk"
                     /locus_tag="PLX80_06735"
     CDS             5263..5820
                     /gene="gmk"
                     /locus_tag="PLX80_06735"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQY20523.1"
                     /translation="MLFVIAAPSGAGKTTIVREILNKIPGLIFSVSATTRKKREKEEN
                     GIDYFFYSKDDFRKMIDKDELIEYEIIFKGDYYGTLKKPVEECLENDKSMVFDVDVNG
                     ALAIKKLFKEKAVLIFIKPPDNETLKERLKKRATESTEQIDERIKRVDLEIGKAKEFD
                     YIVLNDNLEKAVAEVEEIILSNKNK"
     gene            5827..6126
                     /locus_tag="PLX80_06740"
     CDS             5827..6126
                     /locus_tag="PLX80_06740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQY20524.1"
                     /translation="MPIETLDLEKFESKTANLYESIVVASLRSRQINDSMKLEYNQKL
                     EPYIAKEIEDDTIMNQDKLNISLEFEVREKPTIKSVHELMDDKLNFRFKNDNDNK"
     gene            6201..7415
                     /gene="coaBC"
                     /locus_tag="PLX80_06745"
     CDS             6201..7415
                     /gene="coaBC"
                     /locus_tag="PLX80_06745"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQY20525.1"
                     /translation="MLKGKKILIGISGGIAAYKICFLVRYFIKSGAEVRIIMTPSAVN
                     FVSPVTLSALSKNEVLINMFPESADLRKAEKVETKTWHVNLGLWADVFLIAPATANTL
                     AKINFGISDNFLLASILAARCPVFLAPAMDDDMVKNPVTKRNLESLKRFGYNIIDPEF
                     GELASGLTGEGRMAEPETILEIIKNFLLREKDLKNKKILITAGPTVEFLDRVRFITNG
                     STGKMGFEIARAARDRGADVTLISGPVNLNDIEGVKRINVKTSDEMFNAVRSRLKNKD
                     LVIMSAAVEDFVPLKTGKGKIKKEDSEKFIFEFKKAVDILEYAGSKKKEFKLIGFALE
                     TENEIENARIKLKKKNLDMIVSNNPDTKGSGFGTDTNKVNFITNKNIEDLPLMSKYEV
                     GNAIFDRYLKMK"
     gene            7424..8293
                     /locus_tag="PLX80_06750"
     CDS             7424..8293
                     /locus_tag="PLX80_06750"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQY20526.1"
                     /translation="MKDRKNNIVSETIEYLKFVKRYNSFPLFNDSLLVEDFLKKLDEI
                     KRNDCSEVKINLKETLKSNIQKVREEKVYVEVQTEDIEKVVKGVVSTGPELVRDSGVF
                     SIYKNLEELNNIVCECTKCSVLSGGRNSVVFGSGNINADLVVVGEAPGAEEDRLGLPF
                     VGRAGNLLTDILKAVNFSREEVYICNILKCRPPENRNPMADEISNCEPYLFAQLKLIK
                     PKLILALGTFASQTLLRSKEPLGKLRGRFHIYEGIKMMVTYHPAALLRNPNWKKPTWE
                     DVKMLRAEYDKMK"
     gene            8340..9803
                     /gene="dnaB"
                     /locus_tag="PLX80_06755"
     CDS             8340..9803
                     /gene="dnaB"
                     /locus_tag="PLX80_06755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQY20527.1"
                     /translation="MKKNSKYNKKKSNPDKPVNEDMEVEFTGKIPPNAQDLETKILGS
                     ILIDNNVINEILEFLDSKHFYRKKNEYVFDAIKNLDHKKEPIDPNTLKEELQNTGNLE
                     KIGGVEYIIDLTSSVSTSANATYYSRIVLEKYLLRNLISISSEIADKCFNPTSNTFSI
                     LDEAEQKILDVSESLSKKKIISVKDEIDNLMLMLAEQKENKSGLVGIPTGFTRLDDLT
                     AGFQNSELIIIAGRPSHGKTALSMNIARNATVDHGKTVAIFSLEMSFRELILRMLSGE
                     ARVNGKNLKTGKTTTDEWNRVMKSFHKLKTNLYIDDTSELSILEIRAKARKMKKEFGI
                     DMIIVDYLQLVKGPESAERRDLEVAYVSRGLKALAKELEIPVVACAQLNRSIEQRGSK
                     EKRPQLADLRESGSIEQDADVVIFVHRPAMSKSLNKDDPDYLSELRKAEIIIGKQRNG
                     PIDDFDLVFLNEYAKFENKEIHPVIEIPDYIKTDDPF"
     gene            complement(9885..10367)
                     /locus_tag="PLX80_06760"
     CDS             complement(9885..10367)
                     /locus_tag="PLX80_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20528.1"
                     /translation="MKKKLFLIASLVVIVALSIFYVQNNNLIADDKEGKDCSSTCTKT
                     AGTSESKSDCSSKTSSSGVSDDDNAGYAVYEFNTDKITCDACKTGMTSDLKQISGVKN
                     IVYGETCNVSKTTGVKVYFSAEETNPEIISASVKEKGLSGNCEDGSKCTSKKKSDVKS
                     "
     gene            complement(10406..11284)
                     /locus_tag="PLX80_06765"
     CDS             complement(10406..11284)
                     /locus_tag="PLX80_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20529.1"
                     /translation="MKYKKNYLSSVIVSLLAGFIYYYFAVDLQKSVNDVFSIQKMEIN
                     VPDNSEDNLANFYIPQDKSKLNNFFRKVKNKIKRKNAFIESGITKAGLESQAKYQKPI
                     PDKNIDFTFELNKLMKNKKSSTDLNGSNSAKDRNNESAEINSSLKIQRTIINKECYSA
                     TVNKDFKNGNGFEYNYVYQARVNNGKTSGDDVNFKLNNGTISVEENDGEATIELNEDV
                     DESYSEECDNSHYCEYSDSETKVNYTNKWKVNVKSYISNKNKNKDRVIIRNKNECNSD
                     ENEIFIEIHSLDDETL"
     gene            complement(11489..12742)
                     /locus_tag="PLX80_06770"
     CDS             complement(11489..12742)
                     /locus_tag="PLX80_06770"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQY20530.1"
                     /translation="MEQKKLFRTIENIIKEAPKTENDEQLLTYVLEQIIKNEEIKIIG
                     GRLWKLNSEKNSYFLFEQMGDVDIIEKNYELSIDQYPMFKNVGNYRSVIAKETDDYLI
                     DKGIYHYSATGVGERYKFKDNAGEIYFLYEYLIALNATTFIEDDLLNTLNIISMTLSS
                     IIRSKDIESTAKENIQELNKAREIQKSILPEHEMKFGNFDMFGLSIPDKIVGGDFFDY
                     IESEDKERVGIAIGDAASKGISAAAQALYVSGALRMGVEFQIKLTTMVKKINNLVYET
                     FPYERFVTLFYCELINDKKGLCQYVNAGQNPPLFYNSQSGETEMLSATGPVLGPAPYQ
                     KYYSDSINFQLNDILVLYTDGIVEATNENFEFYGEDKLKEVILKYKDLNTREICGKIM
                     QDVNTFSSKGIYSDDKTIVAIKRIK"
     gene            complement(12763..13671)
                     /locus_tag="PLX80_06775"
     CDS             complement(12763..13671)
                     /locus_tag="PLX80_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQY20531.1"
                     /translation="MNRNFSGFSENKFVERIIYYSKKLLDKFDNDHVWIMSSGISFNI
                     LICLLPFSLMLLTILGVYLDSETVQIKLIEYINSIVPLPGQYKERIIFELIDKTKELT
                     NNTFLTGIIGTAGLFWTASGVFSAMREVLKKIFKIDAEQSFIKEKIRDFFLVIISLIL
                     LMLSLAATSIVPIVETYSQGLFGETIILNFFQKLIPIIGGYLLSFCLFYIMYIFIPYW
                     KMNKKVILFTSVCTAFFFELLKYLFGFYILKVANYSRIYGAFATIVISIFFVYYLSLI
                     FVVGAEFGEIYKERNKLKIKVKSKSK"
     gene            complement(13673..15208)
                     /gene="hutH"
                     /locus_tag="PLX80_06780"
     CDS             complement(13673..15208)
                     /gene="hutH"
                     /locus_tag="PLX80_06780"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQY20532.1"
                     /translation="MQKNVFLINGNNLTVQNSIGIVTSGQKISLHSSSEKNIRASRDM
                     VEDWIKNDEVIYGVTTGFGEFKDVKISQKDLKTLQRNLILSHSAGVGKYVPDVIARLM
                     ILFRINSLSKGFSGVRIELINTLIKIFNSDIIPLIPSKGSVGSSGDLSPLSHLALLLI
                     GEGSCKLNGNITESRTALRSKKIKPYELSAKEGLAMINGTQMMSAYICKSVYDAEILS
                     KLSDISGSMSLEALRGTSKAFSEKLQSVRPHPGQKKSASNLRKLLKGSEIMRSHKDCG
                     KVQDAYSLRCMPQVHGAVKDTIAHCKKVLEIEINSATDNPLLFTASGEHIEGGNFHGE
                     PLAFIADFLAIAVSELGNISERRTARLVDGSLSGLPRFLISEGGLNSGLMIAQYTSAS
                     LVSENKVLCHPASVDSIPTSANQEDHNSMGSVSSIKCYDVINNVKNILGIEFLCAAQG
                     IDFLRPLKSGAGTEAAYLYLRKYTKHIYKDLFISEQILNTCKIIYNMDLIKEVEKITG
                     KLN"
BASE COUNT         5690 a         2288 c         2606 g         5251 t
ORIGIN      
        1 attattgcaa gaccctaaaa taatggtcca gattagaaaa atagaatctc ctgatatttt
       61 gttgttttcc cgaattttta tttaatttaa tataaactga tcttaaaaat gattaatctg
      121 atatgaaaat gaaaatattt ctttcacaaa atattattaa aactaaatta ttaaaagtta
      181 acttaccgta aaataacatt atttagtatt gaatattaat attcaggtat tttatgtaat
      241 ttctcaaaat ttttttgtta attttttaaa aattgagtta atttaactat aagtttttga
      301 gaaattcaat ttccagtaaa tgaaatttgt tattttgatt ttaactttga ttctggtttg
      361 tggaaatatt aatgcaaagg aaaagaaatt tattgaaaac gaattcaata acagcattta
      421 tttgggacat caaaaatatt aataatgaat taaaaggatt atttgattct gcaaaagatc
      481 cttttgatcc aatgctgaat gatctggtaa acaggaaagt tcatttactt tttttcgggt
      541 ttaagtttta atccagctat atcagttgta atcgtattta aagctgcaaa atccctgttt
      601 atgggtgagc tttatgaaat aaataaatca tgatctaatc aatctgtttt catatactgc
      661 gaaaggtcag tttaaatttt cttttcagta aaatattatt actgacataa atgactgaaa
      721 tattaattgg taattataga tctagcgaat taactttcag cattggcgaa atttttttaa
      781 aaattatttc ttaaaaacta tattattcat ttataaaaac taattgaaat ctcacagatc
      841 ggtaatttca gttttcatta ttttctttat tgctgctttt acattgcaaa aagaaagtac
      901 agcacaaact aagttaggca taacaaccgg attcacatta tctacaataa gcggtgatgc
      961 gccagataat tctgaatatt cttcaagttc aggattagtt ttgggactta caggtgaatt
     1021 taacattaca aaagatttaa aaataaatct acagccacaa tattttagta ataattcgat
     1081 aatttcttat gatttaaaca gaaaagaacc tattgatagt ctaaaattaa atttccagta
     1141 tattagtctc ccgataattt tgaaagtccc ggcattaagc agcattacat attttaatag
     1201 cggatttagc atttcatatt tgatgaattc aaagatcacg gatattgatg gtgcagatta
     1261 taatgtcgat cttaatgatc ttgtaaataa atttaaccta gctattttat tcggagttgg
     1321 attaaatttt ccggtttata aacaatttga acttgagttt gaagcaagat acactcagag
     1381 tattttgaat ctgagcaacg gatcgacttc atttgtagga acactaccag acagatatag
     1441 atccagcgga tttatgatac tggctgatat aatctattca tttaaataaa aaattgatcg
     1501 atgtttatta aaattattta tacagtttta ttcatatgtg tatttactat taatttgaaa
     1561 gcacaaaaca atagtgatgt taaaatttca gatcgggatt tacctgaaat tactaagcca
     1621 aatttaaacg gacataactt tataattact tcatcattta aaggtccatt cataagtact
     1681 tcaatcaaaa cattattagg tgcagggcag acacttggtc ttgaattacc ggctgtcaga
     1741 ctggatactt ttacattgaa ccgggacatt ggatctattc ttataaccag tcttgatgtg
     1801 gaatatgaag ctcagataag agaatggctg tcattttatt taagttatca gatctatgga
     1861 agattaggaa cgaagacaac cactttggta gcgcagggag tcaatcttgc ttcgggatat
     1921 cagataggta tgatgtttaa gttatgggaa aagaagaata tgcttttatc aggcactgta
     1981 aatgtaagta ataaaagtta tacaattata gatcttgaaa attatataag cggaattata
     2041 gaaaacggat tcgatgaaaa tgatttactt gtttattcca tcccttcatt aagtgtcctg
     2101 ggaggagcaa attatgcctg ggcgatcgac agatcctggg gattagttgc aacattggaa
     2161 ttgggatatg gtgaatcgat caataaatca tcaaatgata attttttaat aaatacaggt
     2221 gtttcagttg attttgatct tcttccaaga agcaaagttc ctattggatt taatcttgga
     2281 tattacaata actcttttcc tgctctatca ggcgatagaa taggaaatcc tcaaaatttc
     2341 tttggacaaa ttgcttatac aggtagaaat gatcttgcgg caggctttga attatcgtat
     2401 caattttcaa aacctgaaag ttacaatact gacggaaaag taacaaaatt tttaacaatt
     2461 tacggcaatt taaaatacta tttttaaaga actggagatg tatatatttc ctacagatat
     2521 tgaattgata taatattgac catagtgtac ttattcagat aaaattcatt ttaacagaca
     2581 aactaatctc ctgcttatgg ttattaattt ttgtattttt cactttatat aaaactgccc
     2641 ttgtagctca ggtggataga gcagcagttt cctaaactgc gggccggatg ttcgagtcat
     2701 cccaagggca caaaattcat agcaaaccta attttcaccc ttaataaata tgaacagcag
     2761 taacaaagtc gcattagtaa ccggcggtgc cagaaggctc ggtaaacata tttcgctttc
     2821 tctggctgaa tccggatatg atattgtcct aaattataac gaaagtggaa aattaatatt
     2881 agagaagatt ttaaagaact tgtcagaatt taatgtaaat gttacagccg taaaatgtga
     2941 ccttactaaa gtgaaagaaa taaaaaaaat gttttcagta gtaagtctga aatacggaaa
     3001 acttgatctg ctggtaaata atgctgcagt ctttaaaaag tctgattttt ttgaaactac
     3061 tgaaaaaatg tatgacaatt ttcttgatac aaatctgaaa ggtatgttct tttgttgtca
     3121 ggaagctgct aagataatgt tgaaaagtga aaataaaatt agtagaataa taaatattgc
     3181 ttcactcgga gcgattgaaa actggacagg gtatatccca tattcattgg ctaaaacagg
     3241 agtaataaaa cttacatcac agcttggaaa aaaacttgct cctgaaatta ttgtaaatgc
     3301 aattgcgccc ggcacaattg aaatggaaga tgaagtaaat gctgataaag aaaatattaa
     3361 caaataccca atgaaaaaat ttgcagatac cggagatatt ctttcattgg taaaatatct
     3421 tgctcttgaa aataaatata ttacaggtca gacctttatt gtagatggag ggaaaagtct
     3481 ctgaaaataa aacaggtaaa tacacaagaa taaattttta aaataaaatt aattaatgaa
     3541 atttttaaac aagtctccga aagcctatca tgatctggat tttttaaatt cacctcaggc
     3601 aagagatatc agaattttaa cagaatataa agagcctctc gaaagattca aaaaacataa
     3661 aatatacaac acagttgttt tttttggatc ggcaagaaca ttaccccgca agactgcttt
     3721 aaaaaatctg aaagaaataa ataatataat aaatggaaat gaaaatgtca gtttaacgtt
     3781 gcaaaaacaa ttaaaaagtg cagagacaga tcttttgatg tcaagatact atgaagagac
     3841 agttgaactt gctgagatga tgactaaatg ggcaatgagt cttggaaagc cgcataagta
     3901 tgttgtttgt tcaggtggtg gtccgggtat catggaagct gcaaacaaag gtgctacaaa
     3961 agcaaaaggt ctttcaattg gtctcaatat atcacttcca tttgagcaat atccgaatcc
     4021 ttacatcact cctgatctta attttgaatt tcattatttc tttatgagaa agttttggtt
     4081 tgcctatctt gcaaaagctc ttgtaataat gccgggtgga tttggaactc tggacgaatt
     4141 gatggaaatt ctgacactta tacagactga aaaactcaga aaaaaattca cagttgtagt
     4201 ctatggtaca gaattctgga ataagattat aaatatgcca atgatggctg aactaaatgt
     4261 gatctcacct gaagatctga aactttttaa atttgtcgat agtcctcagg aagcttttga
     4321 atttcttact aaagaattac ttaaaaatta cattaataaa aagtttaaga aatgattatt
     4381 agcatgactg ggtttgggaa atccgaaggt tcttataaag gtagaaaata ttatgttgag
     4441 atcagatctg ttaataacag atacagtgac ctgagtttaa ggtatccaag acagtttgca
     4501 tcttatgatt ttgaacttaa agaaatcgtt aaaaaaaaaa tttccagagg taagataagc
     4561 gtaaacatca ctgctgaaag tcaggagaca gctatcggaa atctaaattt ggattctgta
     4621 cttataaaaa aatatgcaga agaattaaat aaaattaatt cgcttattgg atctgatgaa
     4681 aaggtcagac ttgagcatat tcttagactt gctgaaatgt cgagtcctga agtttcaaca
     4741 gaatctgatg aatccgaaaa aatttttctg tttaaactta ttgataaggc tgtcgaggat
     4801 cttaacaata tgaaaaagaa agaaggagac gctttaaaaa aggatattat agaaagaatt
     4861 aattttataa aaaaagattc tgaaattatc acaaaacttt catctagtaa gaccagtgtt
     4921 ttaagaaaaa aattaaaaga taaaataaat aatctgcttt ctgacaataa actaatagat
     4981 gaaagcaggc ttgaaacaga gataatttta tatgctgaaa gaattgatat taccgaagag
     5041 ctgatcagat taaagagtca tattaaatat ttcacagaca atattaagtc aagcgaactt
     5101 tccggcaggc gattgaattt tctcacacaa gagataaaca gggaagtaaa tacaatttct
     5161 tcaaaatcta tggacgctga aatttctcaa agaggagctg taatgaaaga agagcttgag
     5221 aagatccgtg aacaattaca aaacgtagaa taaatatatc aaatgctttt tgttatagca
     5281 gctccctcag gagcaggaaa gacgactatt gtaagagaga tattaaataa gatccctggt
     5341 ctgatttttt ctgtttcagc gactacacga aaaaagagag aaaaagaaga aaatggaatt
     5401 gattattttt tttacagtaa ggatgatttt agaaagatga tagataaaga tgaattaata
     5461 gagtatgaga tcatattcaa aggagattat tacggtacat taaaaaaacc tgtcgaggaa
     5521 tgtttggaaa acgataaaag tatggttttc gatgtggatg taaacggtgc ccttgccatc
     5581 aaaaaattat ttaaggaaaa agcagtcctg atcttcataa aacctccgga taatgaaact
     5641 ttaaaagaaa gattaaagaa aagagctact gaaagtactg agcagataga tgagagaata
     5701 aaaagagtag atttagaaat cggaaaagca aaagaatttg attatattgt attaaatgat
     5761 aatcttgaaa aagcagtagc tgaagtagag gaaataattt taagtaataa aaataaataa
     5821 ttaaatatgc caatagaaac cttagatttg gagaaattcg aatcaaaaac agcaaatctt
     5881 tatgaatcaa tagttgtagc ttctttaaga tccagacaga tcaacgattc tatgaaatta
     5941 gaatataacc aaaaacttga gccatatata gcaaaagaaa tagaagacga cactattatg
     6001 aatcaggata aacttaatat atctctggaa tttgaagtaa gagaaaagcc aacaattaag
     6061 tcagttcatg agctgatgga tgataagttg aatttcagat ttaaaaacga caacgataat
     6121 aagtaataca gatctcttta aattttattt cacattcaaa atatcccttt aataacggga
     6181 tatttttttt acatctaaat atgctaaaag gaaaaaaaat actgattgga atatcaggag
     6241 gtattgcggc atacaaaatc tgttttcttg taagatactt tatcaaatca ggagcagaag
     6301 tcagaattat tatgactcct tctgctgtaa attttgtttc tcctgttaca ttatcggcat
     6361 tatctaaaaa tgaagtttta ataaacatgt ttcccgaatc agcagattta agaaaagcag
     6421 aaaaggtcga aacgaagacc tggcatgtaa atcttggttt atgggcagat gtttttttaa
     6481 tagctccggc tacagctaac acacttgcca aaattaattt cggaattagc gataattttt
     6541 tactggcatc aattctagca gcaagatgtc ctgtatttct tgctccggct atggatgatg
     6601 atatggtcaa aaaccctgtt acaaaaagaa atcttgaatc tttaaaaaga tttggataca
     6661 atatcataga tccggaattc ggtgaacttg caagcggact taccggtgaa ggaagaatgg
     6721 ctgaacctga gactatttta gaaattataa agaattttct tttaagagaa aaagatctta
     6781 aaaataaaaa aattctcata actgccggac ctacagttga atttcttgac agggtgagat
     6841 ttattacaaa tggctctact ggaaaaatgg ggtttgaaat cgcaagagca gcaagagaca
     6901 ggggagcaga tgtaacattg atctctggac cggtgaatct gaatgatatt gaaggtgtga
     6961 aaagaataaa tgttaagact tccgatgaaa tgtttaatgc tgtaagatcc agattaaaaa
     7021 ataaagacct tgttattatg tctgctgcag tcgaagattt cgtacctttg aaaactggaa
     7081 aaggtaagat caaaaaagaa gatagtgaaa agtttatttt tgaatttaaa aaagctgtag
     7141 atattctgga atatgcaggg agtaaaaaga aggaatttaa gctgatcgga tttgcgcttg
     7201 aaacggaaaa tgaaattgaa aatgcacgta ttaaattaaa gaagaaaaat cttgatatga
     7261 tagtatctaa taatccggat acaaagggtt ccggattcgg tactgacaca aataaagtaa
     7321 attttattac aaacaaaaat attgaagacc ttcctcttat gagtaaatac gaagttggaa
     7381 atgcaatttt tgacagatat ctgaagatga aatgaaagat gaaatgaaag atcgaaaaaa
     7441 taatattgta agtgaaacaa ttgaatatct gaaatttgta aaaaggtata attcatttcc
     7501 attattcaat gacagcttat tagtagagga ttttttgaag aagctggatg aaattaagag
     7561 aaatgattgt tctgaagtta agattaattt aaaggaaact ttgaaatcta acattcaaaa
     7621 agttagagaa gaaaaagtgt atgttgaagt tcaaactgag gatattgaga aagtagttaa
     7681 aggagttgtt tcaactggac ctgaattagt tagagattcc ggagtattta gtatttataa
     7741 aaatcttgag gaattaaata atattgtctg cgaatgtaca aaatgttctg tactttcagg
     7801 cggaagaaac agcgtagttt ttggaagcgg taatataaat gctgatctgg tagtagtggg
     7861 ggaagcgccg ggagctgagg aggacaggct gggacttcca ttcgtgggta gagccgggaa
     7921 tttattaact gatattttaa aagctgttaa cttttcaaga gaagaagttt atatttgtaa
     7981 cattttgaaa tgcagacccc cagaaaacag gaatccaatg gctgatgaaa tatccaattg
     8041 tgaaccttat ctttttgctc agttaaaact aataaaacca aagctgattc tggcacttgg
     8101 gacatttgct tctcaaacac ttctcagatc aaaagaacct ttgggaaaat taagaggcag
     8161 atttcatata tatgaaggta taaaaatgat ggttacatat catcctgcag cgttactcag
     8221 aaatccaaac tggaaaaaac cgacatggga agatgtaaaa atgttaagag cagaatatga
     8281 taaaatgaaa tgagattatt aaaaaaattt gtacagaaaa aattaaaatt ttataaaaat
     8341 tgaaaaagaa ttcaaaatat aataaaaaga aatcaaaccc tgataaacca gtaaatgaag
     8401 acatggaagt tgagtttaca ggtaaaattc caccaaatgc acaggatctt gagacaaaaa
     8461 tattaggttc gatattaatt gataataatg taataaatga gatcttagaa tttctggatt
     8521 caaaacattt ttacagaaaa aaaaatgaat atgtatttga tgctataaaa aatcttgatc
     8581 ataagaagga acctatcgat ccaaatactt taaaagaaga acttcagaat actggtaatt
     8641 tagaaaaaat tggaggagta gaatatataa ttgatcttac atcctcagtt tcgacatcag
     8701 caaatgcaac ttattattca agaatagttc ttgagaaata tttacttaga aatcttattt
     8761 caatttcttc agagatcgca gacaagtgtt ttaatccgac atcaaataca ttttcaatac
     8821 ttgatgaagc ggaacagaag atacttgatg tttcagaatc tctttcaaaa aagaaaatca
     8881 tatcagtaaa agatgagata gataatctaa tgttgatgct agctgagcaa aaggaaaata
     8941 aaagtggtct tgttggaatt ccgactggat ttaccagact ggatgatctg acagcaggat
     9001 ttcagaattc agaattaata attattgcag gtagaccttc gcatggcaaa acagcgttgt
     9061 caatgaatat agctcgtaat gctacagttg accacggaaa aacagtagca atattcagtc
     9121 ttgaaatgtc attcagagag ttgattcttc gtatgttatc cggagaagcc cgtgtcaatg
     9181 gaaagaatct gaaaacagga aagacaacaa cagatgaatg gaacagagtt atgaaaagtt
     9241 tccataaact aaaaacaaat ctttacattg acgatacaag tgaattatca attcttgaga
     9301 tcagagcaaa agcccgtaaa atgaaaaagg aatttgggat agatatgata attgtagatt
     9361 atcttcagct agtaaaggga ccggagtctg ctgaaagaag agatcttgaa gttgcatatg
     9421 tttcacgggg attgaaagca ttggcaaagg agttagaaat acctgtggtt gcatgcgctc
     9481 agttaaacag atcaattgag caaagaggaa gtaaagagaa aagaccgcag ttagctgatc
     9541 ttagagaatc cggttctata gagcaggatg cagacgttgt aatatttgtt cacagaccgg
     9601 caatgagtaa aagtcttaac aaagatgacc cggattattt gtcagaattg agaaaagctg
     9661 aaatcataat tggcaaacaa aggaatggac cgattgatga ttttgatctt gtatttctga
     9721 acgaatatgc aaagtttgag aataaagaaa ttcatccggt aattgagata cctgattata
     9781 ttaaaactga tgatcctttt taaaatcaac tatttaaaaa taaaaaaaac ccggtcatta
     9841 aagatccggg ttttttaata cctaaattaa aaagaaaaaa tctgttacga ttttacatct
     9901 gactttttct ttgaagtaca ttttgaaccg tcttcacaat ttcctgaaag acctttttct
     9961 tttactgaag cagaaattat ctcaggattt gtttcctcag cagaaaaata tactttaact
    10021 cctgtagttt tggaaacatt acaagtttca ccgtatacta tgttttttac tccgctgatc
    10081 tgttttagat ctgatgtcat tcctgtttta caagcatcgc aagttatttt atcagtattg
    10141 aattcatata ctgcatatcc tgcattgtca tcatcagaaa caccactgct tgaggtctta
    10201 ctggaacagt cacttttact ttctgaagtt ccagcagttt tagtacatgt agatgaacag
    10261 tcttttcctt ctttgtcatc tgcgatcaaa ttattgtttt gaacataaaa aatactcaaa
    10321 gcaacaatta caactagtga agcaattaaa aaaagttttt tcttcatttt tttttttgtt
    10381 taattaataa ataatatttg aaaatttaca atgtttcatc gtctaacgaa tgtatctcaa
    10441 taaaaatttc attttcatct gagttacatt catttttatt tcttattatt actctgtcct
    10501 tatttttgtt tttattacta atataacttt taacatttac tttccattta ttcgtataat
    10561 tcacttttgt ttctgaatct gaatattcac agtaatgaga attatcacat tcttcagaat
    10621 aagattcatc aacatcttca tttaattcaa ttgtagcttc accatcattt tcttctaccg
    10681 atatagttcc attatttaat ttaaaattaa catcatcacc tgaggtttta ccgttattta
    10741 cccgagcttg atatacataa ttatattcaa agccgtttcc attcttaaaa tctttattta
    10801 cagttgcaga ataacattcc ttattaatta ttgtcctttg aattttaagg ctggaattaa
    10861 tttcagcaga ttcattattt ctatcctttg cactattact tccatttaga tctgttgatg
    10921 attttttatt tttcatcagt ttattaagct caaaagtaaa atcaatattt ttatcaggta
    10981 tcggcttttg atattttgct tgtgattcta aaccggcttt tgtgattcca ctttcaataa
    11041 acgcattctt tcttttaatt ttatttttaa ctttacgaaa aaaattgttt agctttgatt
    11101 tatcttgagg gatgtaaaaa ttagcgagat tatcctcgct attatccggt acatttatct
    11161 ccattttctg aattgagaaa acatcattta ctgacttttg taaatcaaca gcaaaataat
    11221 aataaatgaa tccggctaac agggaaacaa ttactgaaga cagatagttt tttttatact
    11281 tcatgataat tgtagttaat taataagtcc tgtttactat tatgaatata ctatttatgg
    11341 agaaaaataa aaacaatttt tttgaacttt tttttaatac attcactgat acatttgaaa
    11401 gaaatcagcg taaatttaaa gttttatatt aattaatctt tcttgtttga taaatctaat
    11461 ctgctgatta tatcaactaa ctgcggtatt acttgatcct cttaattgcg acaatagttt
    11521 tgtcatctga atatatacct tttgaagaaa aagtgttaac atcctgcata atttttccgc
    11581 agatttctct ggtgttaagg tctttatatt ttaaaattac ttctttcaat ttgtcttcac
    11641 cataaaattc aaaattttca tttgtagctt ctacaatccc gtcagtatac aatacaagta
    11701 tgtcattcag ttgaaagttt atgctgtcag aataatactt ctgataagga gcaggtccga
    11761 gtaccggacc ggtagcagac agcatttccg tttcaccgct ttgagaatta taaaataggg
    11821 gaggattctg accggcatta acatactgac aaagtccttt tttatcattg ataagttcac
    11881 agtaaaataa agtaacaaat ctttcatatg ggaatgtttc atatacaaga ttatttatct
    11941 tctttaccat cgtagtcagt ttgatctgaa attcaactcc cattcgaaga gctccggaaa
    12001 cataaagagc ctgagctgca gccgatatac ctttggaagc tgcatctcca atggcaatac
    12061 caactctttc cttgtcttca ctttcaatgt aatcgaaaaa atcaccgcct actattttat
    12121 cagggattga aaggccaaac atatcgaagt ttccaaattt catctcatgt tctggaagta
    12181 tgctcttctg tatctcccgg gctttgttta attcctgaat attttcttta gctgtagatt
    12241 caatatcctt acttctgatg attgagctta atgtcatact gatgatatta agtgtattta
    12301 gcagatcatc ttcaatgaat gtcgttgcat tgagtgcaat caaatattca taaagaaaat
    12361 aaatctctcc tgcattatct ttaaatttat aacgctcacc tacaccagtt gcagagtaat
    12421 gataaatccc tttgtcaatc aggtaatcgt cagtttcttt tgcaattaca gatctgtagt
    12481 ttccgacatt tttaaacatt ggatattgat caatgcttaa ctcataattt ttttcaataa
    12541 tatccacgtc tcccatttgt tcgaaaagaa aataagaatt tttttcggaa tttaatttcc
    12601 aaagtcttcc accgataatt tttatttctt cgtttttaat gatctgttcg aggacataag
    12661 taagaagctg ttcgtcattt tcagtttttg gagcttcttt aataatattt tcaatagtcc
    12721 tgaataattt tttctgttcc atataattaa tttaaataaa ttctattttg attttgattt
    12781 aaccttaatt ttaagtttat ttctttcttt gtaaatctca ccgaattctg cccctactac
    12841 aaaaattaat gataaataat aaacaaagaa tatgctaatg actatagtag caaaagcccc
    12901 ataaatcctg ctgtaattag ctacttttaa aatgtaaaat ccaaacaaat attttagtag
    12961 ttcgaaaaaa aatgctgtac atacagaagt aaatagtatt actttcttat tcattttcca
    13021 gtaaggaata aaaatataca taatataaaa aagacaaaat gaaagtaaat atcctccaat
    13081 tattggtata agcttttgga aaaagttcaa tataattgtt tcaccgaata aaccctgtga
    13141 ataagtttct acaataggta caattgaggt agctgctaat gataacatta gcagaattaa
    13201 agaaattata accagaaaaa aatctctgat cttctctttg atgaaacttt gttcagcgtc
    13261 aattttgaat atttttttca gaacttctct cattgcactg aatacaccgc ttgctgtcca
    13321 gaatagtcct gcagttccta taatacctgt tagaaaagta ttgtttgtta attctttggt
    13381 cttatcaata agttcaaaaa ttattctttc cttatattgc ccgggaaggg ggactatgct
    13441 gtttatatat tctataagtt tgatctgaac agtttcactg tcaagatata cacccaatat
    13501 tgttaaaagc attagtgaaa acggtaaaag acagataagg atgttaaatg aaatacctga
    13561 tgacattatc cagacatggt cattatcaaa cttatcaagg agttttttag aataataaat
    13621 gatccgttcg acaaacttat tttcactaaa gccggaaaag tttctattca ttctaattaa
    13681 gttttcctgt aattttttct acttccttaa ttagatccat attataaata attttgcagg
    13741 tatttaaaat ctgttcactg ataaataaat ctttatatat atgtttagta tattttctta
    13801 aatacagata agcagcttct gtaccggctc cactttttaa aggtctgaga aaatcaattc
    13861 cctgtgcggc acaaagaaat tcaattccta atatattttt aacattatta attacatcat
    13921 agcattttat agaagagaca gaacccatag aattatgatc ttcctgattt gcgcttgttg
    13981 gaattgaatc tacagaagcc ggatgacata atactttatt ctcacttaca agtgatgcgg
    14041 atgtatattg agcgatcatt aatccggaat ttaaaccacc ttcactgata agaaatcttg
    14101 gtagaccgct aagacttcca tcaacaagcc gggcagttct tctttcgctg atattaccga
    14161 gttcactgac agcaattgca agaaagtctg caatgaatgc aagcggttca ccatgaaagt
    14221 ttcctccttc tatatgttcc cccgaagcag taaataataa gggattatca gtagccgaat
    14281 ttatctcgat ctccagaact tttttacagt gagcgattgt atctttcact gcaccatgca
    14341 cctgcggcat acatctgagt gaatatgcgt cctgaacttt accgcagtct ttatgtgagc
    14401 gcatgatctc acttccttta agaagttttc tcagatttga agctgatttt ttttgacccg
    14461 gatgtggtct gacagattgt aatttttctg agaaagcttt acttgttccg cgaagtgctt
    14521 caagtgacat gctgccggat atatcactca gcttagataa aatttctgca tcataaacag
    14581 atttgcaaat ataagctgac atcatctggg taccgtttat cattgcaagc ccttcctttg
    14641 ctgacagttc atatggtttg atcttttttg atcgaagagc agtgcgggat tcagttatat
    14701 tcccgttaag tttacaggat ccttcgccga tcaaaagaag tgctaaatga gataatggag
    14761 aaaggtcacc tgaacttccg acagatcctt ttgaaggtat aagcggaatt atgtcagaat
    14821 taaaaatttt aatcagcgta ttgataagtt ctattctaac tcctgaaaat cctttagaaa
    14881 gtgaattgat tcggaaaaga atcatgagtc tggcaataac gtccggtaca tacttaccaa
    14941 cgcctgctga atgagacagt ataagatttc tttgaagagt tttaagatct ttttgagaaa
    15001 tttttacatc tttgaattct ccgaaacctg tcgttacacc atagattact tcatcatttt
    15061 ttatccaatc ttcaaccata tctcttgacg ccctgatatt cttttctgaa gagctgtgaa
    15121 gagaaatctt ttgaccgctg gttacaatcc cgatcgaatt ctgaacggtt aaattatttc
    15181 catttataag gaaaacgttt ttttgcattt gttaatatag taatgtagta aagaagttta
    15241 aagataattt aggattgata ttacttatgc ttatagaaat gaatttgaaa atgaaaaatt
    15301 atgaacctga tcaattatta gtgagtttgt taaaaaatat ttcagcctca tctttgtcat
    15361 taaaccaata tgaattttcc tttttctcaa aaattaagaa ttttttataa acattaacta
    15421 tccacattgc ggggtctgaa attctgttta ttaattcaaa ggtctttccg ttaacggaag
    15481 ttttttgcct gaaatctttc ataatgattt ttaataattt aaataaatat attcactata
    15541 taaacgttaa tgtttttacg ttttggatta tatattaaag ttgttagtga atatattaat
    15601 aactgtctgc aaataaaaat attaaaaaat atgtatattt ataatcatca tttaaaaata
    15661 aattgaacac atgcatattt tttccgtaac aaaacatttt aaaaatattg aaaaaagata
    15721 atttgtttaa aaagatctat gacttttatt caggcgattt aacttccaaa gaattagaga
    15781 acactttttt taaggaaacg cctgtcagat ataaatatta tctgaaagaa ctgga
//
LOCUS       DAORVQ010000062        15765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1622_length_15765_cov_6.325143, whole genome shotgun sequence.
ACCESSION   DAORVQ010000062 DAORVQ010000000
VERSION     DAORVQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_15765_cov_6.325143"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLX80_06785"
     CDS             complement(<1..937)
                     /locus_tag="PLX80_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQY20533.1"
                     /translation="MGNLFSPTYIICDIETTGLSPFNNRITEIAMIRLRDGEITDKFV
                     RLINPGQHIPYHITKLTGITDPDVFNEPSFEMLARDIMNFLTPESETDNEVFFVGHNV
                     GFDYKFIQHSFQRAGMNFEMKTLCTCKLSNRLLKRLQSKSLSNVAAHLEIKIKKNHRA
                     YDDTLATAKIFQYFLSQLSDKYEYEDVNEVVKFQNSKIYGSENLSPALKRTKISLKEL
                     PNKPGVYFMKSKDGEILYIGKAKDIRERVSGYFRHNSEFPVKLRKLLRNIASLEYEIT
                     NSELSALILESKMIKYHKPRFNSALKRYRHHPFLKI"
     gene            1111..2085
                     /locus_tag="PLX80_06790"
     CDS             1111..2085
                     /locus_tag="PLX80_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQY20534.1"
                     /translation="MRNLWRNILIEFIRKHRLKSNSENTLSVLEIARKLRKLRKSLIA
                     NLKNFIFAIIGISSAAFGLKSFLLPNNFLDGGAMGIAILISELSKISLSILIILINFP
                     FIILGLNVIGKQFVIKTALVIGGLALVMAVFSFPQITNDKLLVAVFGGFFVGAGIGLT
                     IRSGAVLDGTEVLAIYISRKFGTSIGDIIFVINLLVFSSAAYFFSIETALYSIITYLS
                     ASKTVDFIIEGIEQYMGITIISHHSEKVRIMIINVLNRGVTIYTGKNGYGKSGEFKNI
                     DVIYTVITRLEVSKLYSEIRKIDPKAFIVNSSVNDIKGGIIKKRPFNH"
     gene            complement(2230..2796)
                     /locus_tag="PLX80_06795"
     CDS             complement(2230..2796)
                     /locus_tag="PLX80_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQY20535.1"
                     /translation="MRKFTNAILFLFIFTACNIFAQSAEEQKAWMDYMTPGSVHEMLA
                     QSNGTWIGENTFWMAPGAPPITTTSEASNSMIMGGRYQQGLHVGDMMGMPFEGMSLIG
                     YDNAKKQIQSIWIDNFGTGMMYLTGTWDESSQTVTMTGTSVDPSTGKDVKVREIFSIL
                     SPTSQLLEMFMEKDGTEFKTMEIKYTKK"
     gene            3023..5530
                     /locus_tag="PLX80_06800"
     CDS             3023..5530
                     /locus_tag="PLX80_06800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQY20536.1"
                     /translation="MSSISYKGLTQKEVIESAKKFGKNIPETQSKNILLQTIKDIVFE
                     PMFILLVAAALIYFLLGETSEGIIMLAALTIVAGISLFQENRSRNAIDTLKKISESSA
                     KVIRDNNIIEIPSEDIVIGDLIITEEGSKVPADARIIEVHDYSVSESMITGESLPVFK
                     DLTKKGNRIYKGTMVMSGSSIAEVTEIGSNTVYGKIGTSLSDITTEKTPLQIQINKFV
                     KIMVILGSIAFIIVWGINFILTGDIMQSLLQGLTLAMSVLPEEIPVAFSTFMALGAYK
                     LYRERVITKFPQTVETLGTATVICIDKTGTITENKMKLVAVYDYSKDISYDLQNDQMP
                     VNEVLEYAMWSSEPQPFDPMEISIHQYYDKLAANNKKADHKFIHEYPLSGSPPMMTHI
                     FQGKDKEIIVAAKGGAESILANCNLTDIEKNKIISKSDEFAKKGYRVLAVAKSNYQDL
                     NFPESQSDFEFEFLGLTAFYDPPKSNIKNILNDFYKAGIKVKMITGDYSETAMAIAKQ
                     IGFKGYEDIITGKEILELSEDELKVISEKKNIFARMFPEAKLKVVNALKANGEIVAMT
                     GDGVNDAPAIKAAHIGIAMGDRGSEITKKTASMILIDDDLSSMVTAVAIGRRIYENFK
                     KAVQYIISIHIPIILIVTLPLIFFWDFRNIFSPVHVIFLELIMGPTCSIIYENEPIEA
                     NSMKRPPRKMSGNFLSKKELLISTIQGIVITAGCLAAGKYFFDISGDEDLGRTIVFST
                     LIFSNLFLTLVNRSFYYSVFTTLKYKNNLIFLIIGISLIVLTGALFFPPAVALFQLTE
                     LSLMQIIISFLTAMICTGWIEFYKVKVRRGFDIVFMI"
     gene            complement(5844..8273)
                     /locus_tag="PLX80_06805"
     CDS             complement(5844..8273)
                     /locus_tag="PLX80_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY20537.1"
                     /translation="MKKFIILANVVGVIIFGSTYLSAQIYWNQNALFSGSSTSYMSVP
                     NSSSLDITGSITIEAWINPGSLSGGSKGIISKGGALGTSLRYGIRLINAGRIGFYTNG
                     AHRITSSSSSVLQVGKWTHVACSFSSVSDSFKIYINGVLDTAAIIVGAEPQTNPDSVF
                     IGISGNSTPFAGRLDEVRIWNKEVSAEQISKNMRSSLGTSSGIYLGLVLSSAFQREFP
                     SALSARDLSDFGNHGQFRNVSSSPAPESDAAMLPFSPDQNLTLNNCIFFNGDDTYLAG
                     TDTPSLNPVNAVTLECWFYPIENNQGRIITKGNSYAFIYTSGSINALINGALITSGHS
                     IPLKKWSHIAFTYTSTGQYKFVFNGVQVTAGFNNLGPVTVNSDSLFIGGGLGTILDID
                     GYIDELRITNKSKTISEIQNSIYASFNKSSDPNILDNNVNYGFDGNTFDNNDNGGPKL
                     LFRNNTTFSNPGTVESQPVSPVNQDNINIFHRGYYIQQTRKLIPQSGTLRDSQTVNKS
                     VNLQDINLFVSLNHTKLSDLEISLISPHGDSIVVFNNRSTNCSDNNLITIFDDDADSS
                     LSSNSFSSFHAKIKPENNLGNVLMNKNIQGSWKLVIRDEVSLDSGFLYAWGLNFNEEG
                     LRGYDLQVYSLIQGFYDPAANLQQPDTITAILRNSSFPYTAVQSVKTILKTDGEGLFN
                     FTPATAIQNGISFILELSHRNSINTISTGMTFNFNDLKLNIRDSGTLVVGSNVIQVDT
                     SPVRFAIYGGDVNKDGIVDVTDALIVDNNSFNFATGYLASDVTGDNFTDASDASIVEN
                     NAADFVSVIFP"
     gene            complement(8850..10337)
                     /locus_tag="PLX80_06810"
     CDS             complement(8850..10337)
                     /locus_tag="PLX80_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20538.1"
                     /translation="MTKLISIIFVIQILCFNASVFAQVSLYSFTQNNGSYNEITGDTT
                     VAFSTSTITNPMNLDDVTYPSNSIPFSFRFNGAAYTTFAINSNGFITFGTTPPVAANY
                     GPIAATETYDGSVSSFGANLIGIFGTNADRVAGSPVLSNVVSFAGIVAGRLITGTGIA
                     ASATIISFNQSLGTILLSAAATSTGSGEIIQTASASIVRGTTGAAGNRVHTIQFKNFK
                     RNITTGTVDNLNFQIKLYEAAGKIEVVYGNIDQGPISVSGQVGLRGILASDFNNRTTS
                     SNWLTTTAGATNDVQCLISSTVFPSSGLTLIWGPPTANANIKVIPEGFYNPGSEKLNM
                     RDTARVYLHNITSPYAIIDSAKSIIDSVTFSGSYIFSNVSNGTYYLAVKHRNTIETWS
                     KAGGESLTNGGTLNYDFTNLITKAFGNNLILVDPSPVRFAIYSGDVNQDGTVDLTDGS
                     MIDNDAFNFASGYLPTDVNGDGIIDLADAVFADNNGFNFVTKITP"
     gene            11054..12247
                     /gene="kbl"
                     /locus_tag="PLX80_06815"
     CDS             11054..12247
                     /gene="kbl"
                     /locus_tag="PLX80_06815"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQY20539.1"
                     /translation="MFKQFQPELQKELDSIKEQGLFKKERVIETPQGAEINANGKEVL
                     NFCANNYLGLSSHPEVIKAAHETLDKYGYGMSSVRFICGTQTIHKELEKKISEFLGTE
                     DTILYAACFDANGGVFEPLLGDADAIISDELNHASIIDGVRLCKAMRFRYKNNDMADL
                     EEKLKDAKEKGAKQILICTDGAFSMDGVIANLKGICDLADKYEAMVMTDECHCTGFLG
                     KTGRGSIEVNDVMGRIDIITGTLGKALGGAMGGFTSGRKEIIEMLRQRSRPYLFSNSL
                     APAIVGASLKVLELLSETTELRDKLEENTNYFREKMTEAGFDIKPGIHPIVPIMLYDA
                     KLSQDMAAKLLDEGIYVIGFFYPVVAKDKARIRVQLSAAHSREDLDKCITAFVKVGKE
                     LNVIK"
     gene            12266..12721
                     /locus_tag="PLX80_06820"
     CDS             12266..12721
                     /locus_tag="PLX80_06820"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HQY20540.1"
                     /translation="MENLFNTKDRELILERISKLNENSKAVWGKMNVNEMMVHLTDPL
                     RVAIGEKQAEFFKTEYSEPGLRHKIIYELEWPKDSPTSPEFNREFKGTEPMDFKSDLD
                     TLHEVVKNFISKSDEVSFTIHPTFGDISKEDWGRLMWKHMDHHLKQFGS"
     gene            12730..13227
                     /locus_tag="PLX80_06825"
     CDS             12730..13227
                     /locus_tag="PLX80_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20541.1"
                     /translation="MTDRPKYSLIEFERRWLVKKDMIPDISVLEKILITDKYFPDTRM
                     RLREMTNTQEGETVYKLTKKYGKITDSSEPLTTIYLSQAEFDLFNRLDGYGLKKTLYR
                     FPYSGNIFLIEFFSFPITDLILAEAESQSEKELSQLILPDFISKEITTDKNYEGYSIA
                     SGLRF"
     gene            13280..13747
                     /locus_tag="PLX80_06830"
     CDS             13280..13747
                     /locus_tag="PLX80_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20542.1"
                     /translation="MNIDSDEIDRSISGFMNRHGVQALQISLAIIFIWFGILKPLGIS
                     AAAPLVRSTVDWMPFFDADGWVNIIGWWEVTIGVCFLFKKTIRIAIALLAMQMAGTFM
                     PLILLPEVTFQTGKIPYAPTIEGQYIIKNLLIISAALVIGGTVRNRDNKLLKI"
     gene            13747..14211
                     /locus_tag="PLX80_06835"
     CDS             13747..14211
                     /locus_tag="PLX80_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY20543.1"
                     /translation="MIIKIRKAEIKDLTELNELQQSLVNFERPFDDGIPAKGDVNYYD
                     INRLLIDDFTYFVVAETEGKIIGCGFAQIRDNLNWAIEKKLGYIGLMVVREDYRRNNT
                     GKNIIENLTGWLKEKNILHIILETYSCNETAINAYKKYGFKEFVLQMKFDTN"
     gene            14307..15665
                     /locus_tag="PLX80_06840"
     CDS             14307..15665
                     /locus_tag="PLX80_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQY20544.1"
                     /translation="MNLIDRYTLKKHIGPFFFGLFTVIFIFLFQFFIKSLDQFLGKGL
                     SMWIIIQLISLNLAWMLTLAVPMAMLVASLMAFGSMSSENEITIIKASGISLQRLMLP
                     VILISFVMFYLMIKFNNDILPEANHKARILLYDISKTKPTFLLEAGKFSDDLQGIRIL
                     VKKTFPNSNNIEGVFIYDYSDPQYKNLLTAKSGDIGFSSDFKNIIMNLNDGEIHQLSQ
                     KDPAQDYRKIKFEKHRLMFKAEGFGFQNSNENTFSRGDRELSANAMNIVVDSLIVLKN
                     GLDDKFITALTKDIRRLAELDYKDTVFTKPVSEPIQNSNTINSNTPKEIVLSKYDSLK
                     NLQRLITEKSSEYKNTIAFSKQIDKQIDSYEVEIYKKYSIPFACVVFILVGAPLGYRV
                     KKGGFGIAAGLSLLFFLLYWACLIGGEKLADRALVTPFMGMWIANIIIGCLGLYLMFK
                     SS"
BASE COUNT         5166 a         2611 c         2851 g         5137 t
ORIGIN      
        1 caatttttaa gaacggatga tgtctgtatc tttttaatgc tgaattaaat ctcggcttat
       61 gatactttat catttttgat tcaagtatca gagcagataa ttcggaattc gtaatttcat
      121 attctaatga agcaatattt cttaacagct ttctcagctt tacaggaaat tccgagttgt
      181 gtctgaaata tcccgaaacc ctttctctta tatctttagc cttaccaata tataaaatct
      241 caccatcctt acttttcatg aaatatacgc cgggtttatt tggtaattcc ttcagcgaga
      301 tctttgtcct ttttaaagct ggtgaaagat tttcacttcc gtatattttt gaattctgaa
      361 attttactac ttcattaaca tcttcatatt catatttatc cgaaagctga ctcagaaaat
      421 actgaaagat ctttgcagtt gcaagagtat catcatatgc gcggtgattt tttttgatct
      481 taatttctaa atgtgcagct acattactga gagatttgct ttgaagtctt ttgagaagtc
      541 tgttggaaag tttgcaggta catagagttt tcatttcaaa attcataccc gctctctgaa
      601 aagaatgctg aataaattta taatcaaatc ctacattatg acctacaaaa aagacttcat
      661 tgtcagtctc actttccgga gtaagaaaat tcataatgtc acgtgcgagc atttcaaatg
      721 aaggctcgtt aaaaacatcc ggatccgtaa tgcctgttaa ctttgtgatg tgatacggaa
      781 tgtgctgacc cggattaatt aatcttacga atttatccgt gatctcaccg tctctgagcc
      841 tgatcatagc gatctccgtg attctgttgt taaaaggaga aagacctgtg gtctctatgt
      901 cacaaattat atatgtagga ctgaataagt ttcccaaaag aacttttcga tttaggtata
      961 aatataaaaa tatgaataga ttgttactat tgaaaaattt aaaatattta ttaattgaaa
     1021 tattattgaa gctatgaatt attttatttt tatttgaagg ataaaatcat taagttgtaa
     1081 ttcagtttaa aacaaaccaa taaaatttat ttgagaaatc tctggagaaa tatattaata
     1141 gaattcattc gtaaacaccg gctgaaatca aattcagaaa atacattaag tgttttggag
     1201 attgccagaa aactcaggaa attaagaaaa tctttaattg caaatttaaa aaattttata
     1261 ttcgcaatta ttggtatttc aagtgctgct tttggattga aaagtttttt acttccgaat
     1321 aattttcttg acggaggggc aatgggaatt gcaattctta tttccgaatt gagtaaaata
     1381 tctctttcca tactgattat tctcattaac tttccattta ttatactggg cttaaatgtc
     1441 ataggaaagc aatttgtcat caagacggca cttgtcattg gcggactggc acttgttatg
     1501 gcagtgtttt ctttcccaca gatcaccaat gataaattac tggtcgctgt cttcggagga
     1561 ttttttgtag gagcagggat cggcttaaca ataagaagcg gagcagtgct cgacggtaca
     1621 gaagttctgg caatttatat tagcagaaaa ttcggaacca gtataggtga tattattttt
     1681 gtaattaact tactggtatt ttcatccgct gcgtattttt tctcaattga gactgcactt
     1741 tattctatca ttacttatct gtctgcctct aaaactgttg attttattat agaaggtatt
     1801 gagcagtata tgggcataac aattatttcc caccattctg aaaaagtaag aattatgata
     1861 ataaatgttc tcaacagagg ggtcacaatc tatacaggta aaaatggtta cggaaagagc
     1921 ggggagttta aaaatattga tgttatttat actgttatta caagactgga ggtaagtaaa
     1981 ctctattcag aaattagaaa gattgatcct aaagctttta ttgtgaacag cagtgtaaat
     2041 gatattaaag gaggcattat taaaaagaga ccgttcaatc attagtagtt aacagatagc
     2101 agttaagagt taatattaat ttttgaataa atattggatt aaaaatttaa gttcatggat
     2161 tttgcggaaa ttaaaaatcc cctgtaaaat aatttacaga ggattaattc taaataaaaa
     2221 aaaagcttac tatttcttgg tatattttat ttccattgtc ttaaactcag taccgtcttt
     2281 ttccataaac atttccagca gctggcttgt cggacttaat attgaaaaaa tctctctgac
     2341 ctttacatct ttacctgttg aaggatctac tgaagttcct gtcattgtta ctgtctgtga
     2401 actttcatcc caggttccgg tcagatacat cattccggta ccaaaattgt ctatccagat
     2461 ggactgaatt tgtttttttg cattgtcata tccgatcaga ctcattcctt caaaaggcat
     2521 tcccatcata tctccgacat gcagaccctg ctgatatctt ccgcccatga tcatgctgtt
     2581 tgatgcttca ctagtagtgg tgataggtgg cgctccaggt gccatccaga aagtattctc
     2641 tccgatccat gttccattac tttgtgcaag catttcgtgg acacttcccg gtgtcatata
     2701 atccatccat gctttttgct cttcagctga ctgagcaaat atattacaag ccgtaaaaat
     2761 aaataaaaat aaaattgcat ttgtaaattt tctcatttct gatcctttcg tttattagtt
     2821 tgtataaatt aaaataatac aaaataaatt aaaatgaatt tttaaaaatt tttttatttt
     2881 attgtataca aaatatttat tttcttctga atgcatcata tttctttata gcttactata
     2941 gcatttgtat ttgtttaatt tatctgagtc taatttccat tattaaaatc agtctttcct
     3001 gtttaaatat ttaaattttc taatgtcatc tatttcctac aaaggtctca cacaaaaaga
     3061 agttatagaa tctgcaaaga agttcggtaa aaacattccg gaaactcaaa gcaaaaatat
     3121 attactgcaa acaattaaag atattgtttt tgagccaatg tttatattgc ttgtagctgc
     3181 tgctttaatt tatttcttac tcggggaaac aagtgaaggt attattatgc tggctgcatt
     3241 aactattgta gcgggaatct ctctctttca ggaaaacaga agccggaatg ctattgatac
     3301 cttgaaaaaa atatctgagt cctccgcaaa agttatccgg gataataata ttattgaaat
     3361 cccctcagaa gatattgtca ttggagatct cattattacg gaagagggaa gtaaggttcc
     3421 ggctgatgcg aggatcattg aagttcatga ctattctgtc agcgaaagca tgataacagg
     3481 ggagtcactt ccggtattta aagatcttac caaaaaagga aacagaattt ataaaggtac
     3541 aatggtaatg tcaggttcct cgatcgctga agtcacagag atcggatcga atacagttta
     3601 cggaaagatc ggaacatcat tatcagatat aaccaccgaa aaaactcctc ttcagataca
     3661 gataaataag tttgtaaaga taatggtgat tttgggatct atagctttta ttattgtctg
     3721 gggaataaat tttattctca caggagatat aatgcaaagt ctgcttcagg gtcttacact
     3781 tgcaatgtct gttctcccgg aagagatacc tgttgcattc agtacgttca tggctctcgg
     3841 cgcttataag ctttacaggg aaagagtgat cacaaaattt ccacagactg ttgaaaccct
     3901 tggtactgct actgttatat gcattgataa aaccggtacc atcacagaaa ataaaatgaa
     3961 actcgtcgct gtctatgatt attcaaaaga catttcatat gatctgcaga atgatcaaat
     4021 gcctgtgaac gaagtattgg aatatgcaat gtggtcttca gagccgcagc cgtttgatcc
     4081 gatggaaatt tcgatacatc agtattatga taaattagca gctaataata aaaaggcaga
     4141 tcataaattt attcacgaat atcccttgtc aggtagtccg ccaatgatga ctcatatttt
     4201 tcagggtaag gataaagaaa ttatcgtagc agcaaagggt ggagctgagt cgatactagc
     4261 taattgtaat ttaaccgaca ttgaaaaaaa taaaatcatt tctaaatcag atgaatttgc
     4321 aaagaaaggt tacagagttt tagcagtcgc aaaatctaat tatcaggatc tgaattttcc
     4381 tgaatctcaa agtgattttg aatttgaatt tttaggattg acagcatttt atgacccgcc
     4441 taaaagtaac attaaaaata ttcttaatga tttttataag gctgggatta aagtaaaaat
     4501 gatcacgggt gattattctg agacagcaat ggcaatagct aaacaaatag gattcaaagg
     4561 ttatgaagat ataattacgg gaaaggaaat acttgaacta agtgaagatg agcttaaagt
     4621 tatttccgaa aagaaaaata tctttgcaag aatgtttccc gaagctaaat tgaaagttgt
     4681 aaatgcgctt aaggcaaatg gtgaaattgt tgcaatgacc ggtgacggtg tgaatgatgc
     4741 tcctgccatt aaagctgcgc atatcggtat tgctatggga gacaggggaa gtgagataac
     4801 aaaaaaaact gcatcaatga tcctgatcga tgatgatctg agcagcatgg ttacagctgt
     4861 tgcaattggc agaaggatat atgaaaattt taaaaaagca gttcagtata ttatttctat
     4921 acatatccct atcattctta ttgtgacttt accgctgata tttttttggg attttagaaa
     4981 tattttttca ccggttcatg tgatctttct tgagcttatc atgggaccga catgctcgat
     5041 catttatgag aatgaaccga tagaagcaaa ttcaatgaag cggccgccaa ggaaaatgtc
     5101 cggcaatttc ctttccaaaa aagaattact tatcagcaca atacagggaa tagtgatcac
     5161 tgcaggttgt cttgcagcag gtaaatattt ttttgacata agcggtgatg aggatcttgg
     5221 aaggacgata gtattttcga cactgatatt cagtaacctt tttctcacat tggtgaacag
     5281 atcattttac tattctgtat ttacaacttt aaagtataaa aacaatctga tatttctgat
     5341 aataggaatc tcacttattg tacttacagg agctcttttt ttcccgccgg cagttgcctt
     5401 atttcaattg actgaattaa gtttaatgca gataattata tctttcttaa ctgcaatgat
     5461 ctgcaccggt tggattgaat tttataaagt taaagtgaga cggggatttg atattgtatt
     5521 tatgatttag tgtttaatcc aggcttttgt tgctgttcct atatgttcca aagtttctcc
     5581 tgaatttatt atgtcatttg attaaacttt ttagccaaca agaaaaataa cttccggtca
     5641 gaaattctga tctttaataa taattaaatt gattaaagta tgaccactga taagtttatt
     5701 cgaattcaaa tattatattc gcaaaccttt ggaatacaaa cagttgctca aagcaataaa
     5761 atgcttttta gaagaatatt caaactaaca gttttatatc attaatattt tgaacgctgc
     5821 taaaataaaa attgattttg tctctacgga aaaattactg aaacaaaatc agccgcattg
     5881 ttttctacaa ttgatgcatc gctcgcatcg gtaaagttat cgcctgttac gtctgatgcc
     5941 agataacctg ttgcaaaatt aaaagaatta ttatctacta tcagggcatc ggtcacatct
     6001 acaatgccgt ccttatttac gtctccaccg taaattgcaa atctaacagg tgaagtatcc
     6061 acctgaatta catttgatcc gactacaaga gttcctgaat ccctgatgtt tagtttcaga
     6121 tcattaaaat tgaatgtcat acctgtgctg attgtgttta tcgaatttct atgtgacaat
     6181 tcaagtataa agcttattcc gttttgaatt gcagtagcag gagtaaaatt aaacagacct
     6241 tcgccgtcag ttttcaaaat tgtctttacc gattgaacag cagtatacgg aaaagaagaa
     6301 tttcttaata tggcagttat tgtatcgggc tgctgcaaat tagcagcagg atcataaaat
     6361 ccctgaatga gactatatac ctgcagatcg taacctctta gaccttcttc attaaaattt
     6421 aatccccagg catataaaaa acctgagtca agagacactt catcccggat aacaagcttc
     6481 cagctgcctt gtatattttt attcatcaat acatttccca aattgttttc gggtttaatt
     6541 tttgcatgga aggaagaaaa gctgttgctt gataaagagc tgtcggcatc atcatcaaag
     6601 attgtaatga gattattatc agaacaattt gtagatctgt tattaaatac cacaatcgaa
     6661 tctccatgcg gggagataag ggaaatttca agatcggaaa gttttgtgtg atttaacgat
     6721 acaaataaat ttatatcctg aaggttgacg cttttattta cggtttgcga atctcttaat
     6781 gttccggatt gaggtatcaa ctttcttgtc tgttgtatat aatatcctct gtgaaaaata
     6841 tttatgttat cctgatttac tggtgatacc ggctgacttt ccacagtgcc cggattgctg
     6901 aatgttgtat tatttctgaa tagtaatttt ggtccgccgt tatcattatt atcaaaggta
     6961 ttaccgtcaa atccgtaatt aacgttattg tctaaaatat taggatctga gcttttattg
     7021 aatgatgcat aaattgagtt ttgaatttcc gaaattgttt tgcttttgtt tgtgattctt
     7081 aattcatcta tatatccgtc aatgtccagt attgtaccca gaccccctcc tataaaaagt
     7141 gaatccgaat ttacagttac cggacccaga ttattaaatc ctgctgtcac ctgaactccg
     7201 ttaaatacaa atttatactg acctgtcgaa gtatatgtaa atgcaatgtg agaccacttc
     7261 ttcagcggaa ttgaatgacc tgatgttatc aatgctccgt ttattaaagc attgatactg
     7321 cctgaggtat aaataaaagc gtaagaattt ccttttgtaa taattctacc ctgattattt
     7381 tcgattggat aaaaccagca ttcaagcgta actgcgttta ccggattcag agatggagta
     7441 tctgtacctg ccagataagt atcatctccg ttaaagaata tacaattatt tagtgtaaga
     7501 ttttgatcgg gactgaaagg aagcatagca gcatcggatt cgggagcagg tgagctgcta
     7561 acgtttctaa attgtccatg atttccgaaa tccgaaagat ctcttgccga aagagcagaa
     7621 ggaaattctc tctgaaatgc tgacgataaa accaaaccca aataaattcc gcttgaagtg
     7681 ccgagtgacg atctcatatt ttttgaaatt tgttctgccg aaacttcttt gttccagatt
     7741 cttacttcat ccaatcttcc cgcaaaagga gttgagtttc cgctgatacc tatgaataca
     7801 gaatccggat tggtctgcgg ctctgcaccg actataattg ccgcagtatc caatactccg
     7861 ttaatataaa ttttaaatga atccgaaaca gaactaaaac tacatgccac atgcgtccac
     7921 ttgccgacct gaagcacgga agatgagctg ctcgtaattc tgtgtgctcc gttagtatag
     7981 aatccgattc tgcctgcgtt gattaatctt attccatatc ttaatgaagt gccaagagca
     8041 ccaccttttg atatgatacc ttttgatccg ccggatagcg aacccggatt tatccatgct
     8101 tctatagtaa tactgcctgt tatgtcaagc gatgatgaat ttggtacaga catgtagctt
     8161 gtagaactgc cgctgaagag agcattctga ttccagtaaa tttgtgcgga taaatacgtg
     8221 ctgccaaata ttataactcc gacaacattt gcaagaatga taaatttttt catggtttga
     8281 tttattttgt tgaaatatta aaaaaatctt tatataaaag aatcaccggt cttagcggct
     8341 tcagacaatc gaaaccgcag tatcctgacg tcatccctgt attgatgcag tggagtgtat
     8401 gaaatgaaag aagtctgact tagggatgat gtaattcaat tgatacaaaa ttaattgaga
     8461 ttttaaatta ttcaaaatga aaatattccc attcttaaaa attttcttag ttcatttcaa
     8521 ataaatactt tattttttgt ctgaaattca gatttttgtc tgaaatattc tgtttatcat
     8581 taataatttt ttactgtatc atatgaactt ttgagcatgt aatatctcat tcattaacag
     8641 ctgtgtcttc cgatttattt attctctgaa caaacgattt attttgcgag ggatatcagc
     8701 gagtctaaac aataaataaa aaagccgcag gatccttgat cccgcggctt tactgttttc
     8761 aattttgtaa ttgaattttt ttaattttga aaattcattt caaattcatt tcaaattcga
     8821 aatttgaaat ttgtaatttg taattgctct taaggagtta ttttagttac aaaattaaat
     8881 ccgttattat cagcaaacac cgcatcagcc aagtcaatta ttccatcgcc gttaacatct
     8941 gtcggcagat atcccgatgc aaagttgaac gcatcgttat ctatcatgct tccgtccgta
     9001 aggtcaaccg ttccgtcctg atttacatca ccgctgtata tggcaaatct gaccggtgac
     9061 gggtcaacca gtatcaaatt atttccaaac gcttttgtaa ttaaattcgt gaaatcataa
     9121 ttcaaagtac ctccgttggt taaactttct ccaccggctt tactccaggt ttcgatcgtg
     9181 ttcctgtgct ttaccgcaag atagtatgtg ccgtttgaca cattggaaaa aatatatgat
     9241 cctgaaaatg taacagagtc aattattgat tttgcggaat ctataattgc ataaggcgaa
     9301 gtaatgttat gcagataaac tcttgctgta tctctcatat ttaatttttc ggaacccgga
     9361 ttataaaatc cttccgggat tacttttatg tttgcatttg ctgtgggagg accccagatt
     9421 aaagtcagtc ctgacgaggg aaatactgta ctgcttatca ggcactgcac atcattggtt
     9481 gctccggctg ttgtagttaa ccagtttgaa gaagtggttc tgttattaaa atcagaagca
     9541 agaattcctc ttaatccaac ctgtcctgaa actgagatcg gaccctgatc tatatttcca
     9601 tatacaactt caatttttcc ggcagcttcg taaagtttga tctgaaaatt cagattgtcc
     9661 acagttcctg tcgttatgtt tcttttgaaa tttttgaatt gaatagtatg aactctgtta
     9721 cctgctgcgc ctgtagttcc acgaactata cttgctgacg ctgtctgaat aatttcacca
     9781 gagccggtgg atgtagcagc ggctgacaat aaaattgtac caagactttg attaaaagaa
     9841 atgatagttg ccgatgcggc aatcccagtt cccgttataa gtctgccggc tacaatgcct
     9901 gcaaaactta caacatttga aagtaccgga ctacctgcta ctctgtctgc atttgttccg
     9961 aatattccga tcagatttgc tccgaatgag ctgacagatc cgtcataagt ttcagttgct
    10021 gctattggtc cgtaattggc tgctacaggg ggagttgttc cgaaagttat aaagccgttt
    10081 gagtttattg caaaagtagt gtaagctgct ccgttgaatc tgaaagagaa aggaatgctg
    10141 tttgaaggat atgttacatc atcaagattc attggatttg tgatcgtcga agtagaaaat
    10201 gcaactgttg tatctccggt aatctcatta taagacccgt tattttgtgt aaatgaataa
    10261 agagaaacct gtgcaaaaac actagcattg aaacacagaa tttgaattac aaaaattatt
    10321 gaaattaatt ttgtcatgaa attttagatt gaaatttcag aagacaaatt tttactttga
    10381 ttaaattgca tcaaaaaaaa attaatttaa ggatatggca gtcttctaaa ataatttaga
    10441 aagatttttt aagatatgtt tgtttaagta tcttccttta ggattttaac ctaatgaata
    10501 agctcttttt tattaagcag tgatttgcca atctcagcgc ttatttactt taccggaaat
    10561 acttttttgg ggaagagtat taatcttaat tagaagcaaa acatttgatg acaagaaagc
    10621 taaatcagtt gtataaatta ttggacttgt ctctttttga ttttcttcta ctaaaagaaa
    10681 tttagtgaat gatcggattt tttaattcac aactatcact ttaacaagct atgcttttca
    10741 atctctaaag tcaagcgtta tagttactga ggaagaggtt cgcattgagt catgattaat
    10801 gtaacagtgt tatagcagca aaaacgggat aattcgaacc gtagttggta ttccaaaaag
    10861 ttttgcaatc tcattagaat tttgccattc ctttcgctga aactttttgg tcactaacgc
    10921 caaaactacc agtcagaaac ttctgactat tttaaaactc aactctaatt attgaatcaa
    10981 actctttcta aaaaagtctt attttataga aatcaaagat ttaaaaatat tttactcaaa
    11041 ataatctact tttatgttca aacaatttca acccgaactt cagaaagaat tagattcaat
    11101 taaggaacag ggacttttta aaaaggaaag agttatagaa acgccgcagg gagctgagat
    11161 caatgcaaac ggcaaagaag ttctgaactt ctgcgcaaat aattatctcg gactttcatc
    11221 acatcccgaa gtcatcaaag ctgcacacga aactctcgat aaatatggtt atggaatgtc
    11281 gtcagtaaga tttatctgcg gtacacagac cattcataaa gaactcgaaa aaaagataag
    11341 tgaatttctc ggaacagaag atactattct ttatgcagcg tgcttcgatg caaacggcgg
    11401 tgtatttgag ccgttgctcg gtgatgctga tgcaattatt tctgatgaat taaatcatgc
    11461 aagcattatc gacggagttc gattatgcaa ggcaatgaga ttccgttata aaaataatga
    11521 catggctgac cttgaggaaa aattaaaaga tgcaaaggaa aaaggtgcaa aacaaattct
    11581 gatatgtact gacggtgcat tttctatgga cggcgttatc gcaaacctga aaggaatatg
    11641 tgaccttgct gataaatatg aagcaatggt aatgacggat gaatgtcact gtacgggttt
    11701 tctcggaaag acaggcagag gatccattga agtaaacgac gtgatgggaa gaattgatat
    11761 aattacaggt acactcggca aagcacttgg cggagcaatg ggaggattta caagcggcag
    11821 aaaagagatc attgaaatgc tcagacaaag aagccgtcct tatttgtttt caaattcact
    11881 tgctcctgct attgtcggtg cttcgctgaa agtgctcgaa ctccttagtg agactacaga
    11941 attaagagat aagctcgaag agaatacaaa ttatttccga gaaaaaatga cagaagccgg
    12001 atttgatatt aagccgggta ttcatccgat agttccgatc atgctgtatg acgcaaagct
    12061 ctcgcaggat atggcagcaa aattacttga tgaaggaatt tacgtcatcg gatttttcta
    12121 ccctgtagtt gcaaaagata aagcgaggat cagagttcag ctttcagctg cccattcaag
    12181 agaggatctg gataaatgta taacagcgtt tgtcaaagtg ggtaaagaac ttaatgtaat
    12241 aaaataattt aaaaagaata aagtattgga aaatttattt aatacaaaag acagagaatt
    12301 aatactcgaa agaatttcta agttaaatga aaattcaaaa gctgtctggg gtaaaatgaa
    12361 tgtcaacgaa atgatggttc atctcactga cccgctgagg gttgcgatag gtgaaaagca
    12421 ggcggaattt tttaagacag aatattctga acccggactg cgtcataaga taatttatga
    12481 actggaatgg ccgaaagatt ctccaacttc tcctgagttt aacagggaat ttaaaggtac
    12541 agaaccgatg gattttaaaa gtgatcttga tacacttcat gaagtagtaa aaaattttat
    12601 tagtaaatcc gacgaagtaa gttttacaat tcatcctact tttggtgata tttcaaaaga
    12661 agattgggga cggcttatgt ggaaacatat ggatcatcat ttaaaacaat tcggatcata
    12721 attataaaat tgactgacag accaaaatat tctctgatag aatttgaaag aaggtggctc
    12781 gtaaaaaaag atatgatccc tgacatttca gttctggaaa aaattctgat aacagataag
    12841 tattttcctg atacaagaat gagacttcgt gaaatgacaa atactcagga aggtgaaaca
    12901 gtttataagc tgacaaaaaa gtatgggaag ataacagata gttccgaacc tcttactacc
    12961 atttatttgt ctcaggctga atttgatttg tttaacagac ttgatggata tggactgaaa
    13021 aaaactttat accgatttcc ttattcaggc aatatttttc tgatagagtt tttttcattt
    13081 cccattacag atcttatatt agcagaagcg gaatcacagt ctgaaaaaga actcagtcag
    13141 ttaatactgc ctgattttat ttctaaggaa atcacaacag ataaaaatta tgaagggtat
    13201 tcaattgcat caggattaag attttaataa aataaaacaa tttaatcccc gttcattatc
    13261 atctattaat taaaaaaaaa tgaatataga ctctgacgaa atagacagga gtatttccgg
    13321 atttatgaac cgtcacggag tacaggcatt gcaaatatcc cttgctatta tttttatctg
    13381 gtttggaata ttaaaacctc ttggcatttc tgctgctgca ccgcttgtca gatctacagt
    13441 ggactggatg ccattctttg atgcggacgg ctgggtgaat ataatcggtt ggtgggaagt
    13501 gactatcgga gtatgttttc tttttaaaaa aacgatccgg atcgcaattg ctttactggc
    13561 aatgcaaatg gcagggacgt tcatgccgct tatacttctt cccgaagtaa cttttcaaac
    13621 tggaaagata ccttatgctc cgaccattga aggtcagtac attattaaaa atcttcttat
    13681 catatctgca gcacttgtga tcggcggaac ggtaagaaac agagataata aattattgaa
    13741 aatttaatga ttataaaaat ccgtaaagct gaaataaagg atctgactga actaaatgaa
    13801 cttcagcaga gtcttgtaaa ttttgaaaga ccttttgatg acggtatacc tgcgaaaggt
    13861 gatgtaaatt attatgacat taatagatta ttaattgatg actttactta ttttgtagtt
    13921 gctgaaactg aagggaaaat tatcggctgc ggattcgctc agatcaggga caatctaaac
    13981 tgggcaattg aaaaaaaatt gggatatata ggactgatgg tggtcaggga agattacaga
    14041 agaaacaaca caggtaaaaa tatcattgaa aatctaaccg gatggctaaa agaaaaaaat
    14101 attttgcata tcattctcga aacttactca tgtaatgaaa ctgcgatcaa tgcatataaa
    14161 aaatacggat tcaaagaatt tgttttacaa atgaaatttg ataccaattg aatgttatgt
    14221 ccttttgatc tattttatta gccaatgaat ttttttaatc atttgaatca atttaatcat
    14281 ttgaatcaat tgaaatattt gaaataatga atttaataga caggtacaca ttaaaaaaac
    14341 atataggtcc gttcttcttc ggattgttta ctgttatatt catttttcta tttcagtttt
    14401 ttataaagtc acttgatcag ttccttggta aaggtttgag catgtggatc ataattcagc
    14461 tcatctcact gaatctcgct tggatgctga cactcgccgt accaatggca atgcttgttg
    14521 catctcttat ggcattcgga tcgatgtcat ccgagaatga gatcactatc atcaaagctt
    14581 caggtataag tctgcagaga ttaatgctgc ctgtaattct aatatcattt gtcatgtttt
    14641 atctaatgat aaaattcaac aacgacatac tgcccgaagc taatcataaa gcaaggattc
    14701 ttttgtatga catttcaaaa accaagccga cctttcttct cgaagcaggt aagttttccg
    14761 atgatctgca gggtatcaga atattagtta agaagacatt cccaaatagt aataatattg
    14821 aaggtgtttt tatttatgac tattcagatc cgcagtataa aaatttactt actgcaaaat
    14881 cgggagacat tggattctca agtgatttta aaaatataat aatgaatctg aatgacggag
    14941 agatacatca actcagtcag aaagatcctg cacaggatta caggaaaatt aaatttgaga
    15001 agcaccggct aatgttcaaa gctgaaggat ttggatttca gaattcaaat gaaaatactt
    15061 tttcaagagg ggacagagag ttatctgcaa atgcaatgaa tattgttgtt gatagtttga
    15121 tagtattaaa gaacggtctt gatgataagt ttattacagc actaacgaaa gatattagaa
    15181 ggctggcaga attagattac aaagatactg ttttcacaaa gcccgtaagt gaaccaattc
    15241 aaaattcaaa tacaataaat tcaaatactc cgaaagaaat tgtattgtca aaatacgaca
    15301 gtcttaaaaa tctgcaaaga ctaataacag agaaatcttc tgaatataaa aatacaattg
    15361 cattttcaaa gcagatagat aagcagatag actcatacga agtagagatt tataaaaaat
    15421 attcgatccc atttgcctgc gtagtattca tacttgtagg tgctcctctc ggatacaggg
    15481 taaaaaaggg aggattcggt attgcagcgg gattatcttt attatttttt cttctctact
    15541 gggcatgcct gataggaggt gaaaagcttg ctgacagggc attagtgaca cctttcatgg
    15601 gaatgtggat tgcaaatatt attataggat gcttaggttt gtatttaatg tttaaatcat
    15661 cttaattata ttacaaaagt ttacaatcaa gtttaattgt caagccccga acaatataaa
    15721 attacccaac tccgccaggt tcggaagaaa gcaacggtaa gtaac
//
LOCUS       DAORVQ010000063        15700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1633_length_15700_cov_6.375967, whole genome shotgun sequence.
ACCESSION   DAORVQ010000063 DAORVQ010000000
VERSION     DAORVQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15700
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1633_length_15700_cov_6.375967"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLX80_06845"
     CDS             <1..711
                     /locus_tag="PLX80_06845"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HQY20545.1"
                     /translation="YKPEITDSEILLRNRDLILKSLEKISVILQRNNADTENINVIIF
                     AGQSGANGHAFIYDNKSYAWIAAECYTSELYCDVMCSHEIGHALHYSTVPELSFRNMY
                     EKELVKRQLITEGLASYFTKQIFGTNDITALWADHCDKTTGEKWMNNFETKSSEIIKF
                     VKENFYRSDSSGKLFSMIEDINISNSDPFMSRGGYLLGLKLTEYIADEYNENLFSLLK
                     MPAKEIEGKFYEALKNFN"
     gene            858..3002
                     /locus_tag="PLX80_06850"
     CDS             858..3002
                     /locus_tag="PLX80_06850"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20546.1"
                     /translation="MKIIIIFLTAVLFISTDLSFAQNYWTGLNGPFGGNVLEVNKGNN
                     GNMVILRGEGVYNSADNGETWSAIPQGMVNTNLSMTVSPSGIIYTGKSTGGLWWTGNN
                     GQSWSFNPISVAPHSGLWASIYLVKVSPAGQVYINNHVSFNGGNNFTAFSYNGTNLLT
                     SDYAFNSSGHVYSATTNGIYYSTNNASSWTNINGNLPSLSSSGILTDNNTLITAIHGS
                     GIFKTSDNGNVWTEINNGINDLNIIKIYKDNSGNYFAGTSTGKVFRSSDQGNSWNHIF
                     ISSSANRINSIYSEGNNVVVTSSFGIFFSDNSGITWSERNNNLNIPIINTIIFPGENQ
                     IMAASGTGVQFSDDNGQSWENRSTDLPGMYVNSLLKTDNNEVLASVRNSGIYRTSDNG
                     ITWNNSGTGIPEAYQSADILISPEGYIFAGVNTIEFPDTLKIFHSSDNGNTWSKIYQP
                     ETNDFNTLEIDASGNLYLFKLQSFKTALLRSSDNGNTWTEIDLSEFFFVSEFTSSQNN
                     LYISVSGNIYKSIDMGINWTEIPRGGWQTSSLNALSVNKRGDIFVSAGQDIYISLNDG
                     VSWIDAGSGITNNAYINNFVFDNLNYIYGLNYYYGIFKSAQPTITGISTNLSNSEIPD
                     SYRLSQNFPNPFNPSTVINYHIPVSGKVKLKVTDISGKEVAMLVNEFQTKGDYKAEFN
                     SDNNNISLSSGIYFYTLETAEFISTRKMILLK"
     gene            3289..3597
                     /locus_tag="PLX80_06855"
     CDS             3289..3597
                     /locus_tag="PLX80_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20547.1"
                     /translation="MKNYFSKIFAVYILILAIIPCTDFISHGLCEDTDNIHIENSTES
                     EHEDLCTPMCTCNCCNSIVTLTEQFSLKNYIPHSEILLTEVSQYDDSFSMNTSPPPKS
                     "
     gene            3675..6821
                     /locus_tag="PLX80_06860"
     CDS             3675..6821
                     /locus_tag="PLX80_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQY20548.1"
                     /translation="MINKIIAFSIKQKLVIGFLILVLILLGIYSSVKLPIDAVPDITN
                     NQIQIITSSPTLSATEVERFITYPVEIAMKSLPDIIEMRSISKFGISVVTVVFEDNID
                     TYFARNLVFQKLVEAEENMPEGLGKPEMAPVSTGLGEIYQYIVRPENLNDTTYSDMEL
                     RTIQDWVVKRQILGTEGVAEVNSFGGFEKQYHILVNPDKLKSFDLTLREVFDAVLNNN
                     SNVGGGYIEKNSDQYSIRGIGLIEKTEDLDNIVIKTIHGIPVYLNQIAEIEFGKGLRV
                     GGVTQDGNGEVVAGIVMMLKGANSREVAQRVHEKISEIKTTLPDGVTIDEFYNRENLI
                     DSTIDTVKTNLIEGGVIVIFVLVLLLGNLRAGFIVASVIPLSMLFAIIMMNIFNVSGN
                     LMSLGAIDFGLIVDGAVIIVESVLVALSAKVYLNNDPKSNIEFNDTIYSSSTGIIKSA
                     IFGVLIIIVVYLPIFALGGIEGKMFKPMAFTVGFALVGALILSITYVPMMCSLILKKN
                     MSEKETIADRIMKGLKKLYTPSLEFALRNKILTIAAALIFLIISLISFTNLGGEFIPK
                     LDEGDLAYQIVRIPGVSLTESINIGTQIEKILMEKFPEIKTVVTKTGAPELATDPMGP
                     EFSDVIVMLKPKDEWSSAETKEELIEKIQKSLSVIPGVGLSFTQPIELRFNELIAGAR
                     GDIAVKIFGDDLTVLSEEGNKVASIMGSVKGSEDISVQQVEGLPQLQIKIRRDRISVY
                     GINVDEVNNVIETALAGKTSGVVFEGDKKFDIVLKYDPSYTSNIESIRNILVSSPDNV
                     MIPLSELADIDIKEGPAEITRDNGKRRIVIQGNVRGRDIESFVNELKSDIDMEMKLPP
                     GYVIEYGGQFKNLESARERLMIAVPVSLFLIFALLFLTFNSVKQGLMVFSGIPFAVVG
                     GIFALVIRDLPFSISAGIGFIALFGVAVLNGIVMIAYFNRLEDEGISNVHDRIVSGSA
                     SRLRPILMTALVASLGFIPMALSTGSGAEVQRPLATVVIGGLISSTLLTLIVLPVIYG
                     IFNKHKKHQNPELIKSLN"
     gene            6915..8135
                     /locus_tag="PLX80_06865"
     CDS             6915..8135
                     /locus_tag="PLX80_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY20549.1"
                     /translation="MIKIILFTYFTLFFSQGIISQTKMDMDNIIKVAVENNQSLKTAR
                     LNIDKENATGLRSFNIPKPEIFVEYEGVQGSLSNAESRKIGFLQQFEFPTNYFLRSDM
                     QNSQISVAEQELNAKLNELKAELKMNYNRLQLLYELIRSSEENLNIYSEFLNTAEKKY
                     EAGETSNLEVLGAKVNKIKFQNEITNYKTEIISVQSELRNLMNVNYDVVPTDEMNFKE
                     LNLNRASILNNAETNNPEIKVAGFQKEKFSNKISLSKNELLPDISFKYFRQKIGGQND
                     YWGMEIGLGVPLWFWWEQSGNIKESNYEYQIATSEELSLKKSVENQTDIAFSEYENSL
                     RQVKFFKEEALSESEEILRQSKISYVEGEIGYVEYLQALNLAYETMTQYLNAVYTYNQ
                     SIIKLEKLTASELK"
     gene            8195..9487
                     /locus_tag="PLX80_06870"
     CDS             8195..9487
                     /locus_tag="PLX80_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY20550.1"
                     /translation="MILILAMQGCNSNENNSSEKVDDHENHEDENAGHKDEVSLTEDQ
                     IRVMDIKFSKFEPRNINGYIKVNGEIMMDQDNESRVGSLISGRVKKIFVKEGSYVRAG
                     QTLAVIENPDLINVQVDYINAKNEYEYSKQEYDRQRKLSSDNIGSKKNFAEIETNYKR
                     ALANYRSLEEKLSGYKISKNRFDNIYTDTTADLQRNYSVTAPISGNIVARMVTIGQYI
                     EPSVDMFQIVNTSTVYVDLSIFEKDLGNVKVGQKVVIETGSKPDENYEGKVSFINKIF
                     NDRDRTVKVRVVINNRSQELYPYMFVSAKIYVTGGEALSVPVSSIETEGEINYIFLKT
                     GERKVIDEHDEHEKDNEHEHSDGEKEHTESDHSNEGHTEESGIVFKKVIVNTGITDGA
                     FVEIIPVSEMNLGDEVVSKGTFYLKSELSKGELGEHNH"
     gene            9865..12384
                     /locus_tag="PLX80_06875"
     CDS             9865..12384
                     /locus_tag="PLX80_06875"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQY20551.1"
                     /translation="MNPILRNILGFIILLIIVLVSIGILFNNLTKKSYYDESGTFKVK
                     GITGNVNVYTTELGVPHIYAQNEEDMYFTLGVMHARDRLWQMDLYRRVAEGRLSEIMG
                     KNVLEFDILFRTLAIDKNSRYQYDKLSFKSKSILNSYSAGVNYFIDENSSRLPLEFDI
                     LNYKPDQWKPEHSLMLVKLMAWELNLSWYTDVMFGEIVKKFGVENAKYFFPDYPEDAP
                     FIVKDDQSKIKTDSVKKPTVSPVSDLFKSKNYGDISALGKNYFQSSLNFRKFFGTEGT
                     HIGSNSWVISGSKTETGKPILANDPHLALQVPSKWYEVSLFNEQNKNSICGFSIPGVP
                     GIAIGHNDMISWGLTNLMCDDSDFYILKRDSSVSSKYIYRNTSFGLDSTVESIKIKDE
                     KDEHVITVYSTVLGPVVSKLGTTGFLNRQNFKTENENEFLTFKWTGYEYSDEIETFYK
                     LNNAGNWNEFRDALKTFGAPALNFVYADTSGNIGYRSAGLIPVRNFTESEYPPYTPQN
                     SGALWTGFVPFEEMPESVNPEAGYIVTANNRPKKDYKYYISNLYEPPYRAERIEELIK
                     SIPLVNEDETKKIQRDVYSIQAKEFCEFLFEAFGDSSDITPDIKSYLDILKQWDYEFR
                     LSSTGASIFAMFEVQLYKNLYQNELGSDLFENYLYLKNVPVRNTSKLLKLTSSFLFEN
                     NVIKRQIIRRSFYEAVSALIAKYGNDPAGWRWELMHDVYMKHPLGIVPEFSSMLNIGP
                     FSIGGNGTTINNLEYSFSKALADQRFESYLGPSMRMISSLNSIKNYLSILPTGQSGQP
                     INRNYNDQARLWLNGDYKKVTTDFAELKKDNLKMLSFEPGE"
     gene            12461..13300
                     /gene="rsmA"
                     /locus_tag="PLX80_06880"
     CDS             12461..13300
                     /gene="rsmA"
                     /locus_tag="PLX80_06880"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQY20552.1"
                     /translation="MISNQSKYEKYRPKKFLGQNFLTDENLAKKIVNSLDIKPDDTVV
                     EIGPGQGVLTKYLANLTDDFFAVELDRQIAGELSEKFENKMTVIHKDFLKVDITEEIF
                     SKRKNKGRLKIIGNIPYNITTEIIFLLTENRNLISTAVLMMQREVAKRLTSPHGNKDY
                     GILAVLIQLYSIPKILFNVPPEVFFPKPRVTSSVVKFDFPENIHSCINYALLSKVVKK
                     SFGKRRKTMRNSLKDFFMEKEIAQENIDFDFTRRPESLSIDEFLKLTEDIEKEIFDNE
                     SRK"
     gene            complement(13721..14170)
                     /gene="tadA"
                     /locus_tag="PLX80_06885"
     CDS             complement(13721..14170)
                     /gene="tadA"
                     /locus_tag="PLX80_06885"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQY20553.1"
                     /translation="MIGHDTFMNYAYREAETAYEEQEIPVGCVIVFQNTIIAKAHNQI
                     ETLKDPTAHAEIIAITSAAEYLQSKQLVGCSMYVTLEPCAMCAGAIVLSKIENLYFGA
                     FDNKSGACGSVLNITNNKQLNHNCNVTGGILDDKCREILRSFFDVKR"
     gene            complement(14183..15283)
                     /locus_tag="PLX80_06890"
     CDS             complement(14183..15283)
                     /locus_tag="PLX80_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20554.1"
                     /translation="MANTEKYKVILITGLGVILLILTFIFINYDPDKVEPENSLENKA
                     AITPEKFKKDIDSIIYSFGIKEEWKIKVPENKVSISGDKGLILAQSIRIPVDIQTINL
                     NLDITNYLEENGFISKTSEDPRSKDILIEILQVKDSLQTGYAKLNFIYSKDISRNVSE
                     TAIVLDSIEYIDFQNAENIIKSVEKFSVFLPIGNDKADYQSMIIDNRKNYLLKFYIGS
                     NDDITSDFRTEMKENVIRQKVRTAAVNFPGVSGVVLYYPKGMKEFAEKISSEFKKNNF
                     TVFSDTLFKAIKTEENKVSKLFEEIKKNSEKGIKVQFYTVSFSEKDFREFENRTEELK
                     KPGYKFISINEAVKMISDQNPALDKDTLSVKK"
BASE COUNT         5310 a         2551 c         2890 g         4949 t
ORIGIN      
        1 tacaaaccgg aaataaccga ttccgaaatt cttcttcgga acagggacct gatattaaaa
       61 agtttagaaa aaatctctgt gatacttcag agaaataatg cagacactga aaatattaat
      121 gtcattatat ttgcaggtca gtcaggggct aatggtcatg cgtttatcta tgacaacaaa
      181 agctatgcat ggattgctgc ggaatgttac acttcggaat tgtattgtga tgtaatgtgc
      241 tcacacgaaa taggacatgc gcttcattat tctaccgtac ctgagttgtc tttcagaaat
      301 atgtatgaaa aagaacttgt caaaagacaa ttgataaccg aaggtttagc atcttatttt
      361 acaaaacaga tatttggtac aaatgatatc acagctttat gggcagatca ttgtgataag
      421 actacgggtg aaaaatggat gaataatttt gaaaccaaaa gttctgagat cataaagttt
      481 gtaaaagaaa atttctaccg gtcagacagc tccgggaaat tattttcaat gatagaagat
      541 attaacatca gtaactcaga tccctttatg agcagaggag gttacctgct tggtctaaaa
      601 ttaacggaat atattgcaga tgaatacaat gagaatttat tttcactttt gaaaatgccg
      661 gcaaaggaaa ttgaaggaaa attttatgaa gctttgaaaa attttaattg aattactttt
      721 attaaatgaa cataaaatta gtctgcagtc atttgtattt ttgaactcta catttatacg
      781 aatatttgtt tgtataataa gatcagataa tttaattttg aataaaagat tttaacaatt
      841 aatcaaattt aattttcatg aaaataatta taatattttt aacagcagta ctttttataa
      901 gtacagatct ttcgtttgca cagaattact ggacaggact caatggtcct tttggtggaa
      961 atgttcttga agttaataaa ggcaataatg gtaacatggt aattctcaga ggtgagggag
     1021 tttataattc tgcggataac ggtgagacat ggagtgcaat tccgcaggga atggtaaata
     1081 caaatctcag tatgactgta agtccttcag gaattattta taccgggaaa agcaccggcg
     1141 gtctctggtg gacgggcaat aacggacaaa gctggagttt taatcccatc agcgttgcgc
     1201 ctcacagcgg actttgggca agtatctatc ttgtaaaagt cagtccggct ggacaggttt
     1261 atataaacaa tcatgtttcc tttaacggag gaaataattt tacggcattt tcttataacg
     1321 gaacaaatct cttaacaagt gactatgcat ttaattcttc aggtcatgtt tactctgcca
     1381 caactaacgg aatttattat tcgacaaaca atgcttcttc atggacaaat ataaacggta
     1441 atcttccttc attatcttcc tccggaattt taaccgataa caatacttta ataactgcaa
     1501 ttcatggttc cggaatattc aaaacttcag ataatggaaa tgtgtggact gaaattaata
     1561 acggaataaa tgatctgaat attataaaaa tttataaaga caattccggt aattattttg
     1621 caggtacatc tacaggtaaa gtattcagat cttccgatca gggaaacagc tggaatcata
     1681 tatttatcag ttcttcagct aacagaatca attcaattta ttcagaagga aataatgttg
     1741 ttgttacatc atcatttgga attttctttt ctgataacag cggtattaca tggagtgaaa
     1801 gaaataataa tctgaacatt cccattataa acactatcat ctttccggga gagaatcaga
     1861 tcatggcagc ttcaggaaca ggtgtacaat tttcagatga taatggtcag agctgggaaa
     1921 acaggtcaac ggatcttccc gggatgtatg taaattcact tttaaaaact gataataatg
     1981 aagtacttgc aagtgtaaga aattcgggaa tttacagaac ttccgataat ggtatcacgt
     2041 ggaataattc aggtaccgga attccggaag catatcaatc agccgatatc ctgatctctc
     2101 cggaaggtta tatttttgca ggtgtgaata caattgaatt tccggatact ttaaaaattt
     2161 tccactcgtc cgacaatgga aatacctggt caaagatcta tcagccggaa acgaatgatt
     2221 tcaatacatt agaaattgat gcatccggta atttatattt atttaaactt cagtcattta
     2281 aaacagcatt gctgagaagt agtgataatg gaaacacatg gactgaaata gatttgtcag
     2341 agttcttttt tgtatctgaa tttacttcat cacaaaacaa tctttacata tcggtttcgg
     2401 gaaatattta caagtctatt gatatgggaa ttaactggac agaaattccg agaggcggtt
     2461 ggcagacttc atctttgaat gcgctttctg tgaacaagcg aggtgatatt tttgtatcag
     2521 ccggacagga tatttatatt agtttgaatg atggtgtgtc atggatcgat gccggatcag
     2581 gtattactaa taatgcttac ataaacaatt ttgtatttga taatttgaat tacatttacg
     2641 gattaaacta ttattatgga attttcaaaa gtgcacagcc gacgatcaca ggtatttcca
     2701 ccaatttaag taattcagaa attccggatt cctatagact ttcacaaaac tttccgaatc
     2761 cgtttaatcc atcaacagta attaattatc acattcctgt ttctggaaaa gtaaaattaa
     2821 aagtaactga catttccgga aaagaagtgg ctatgctggt aaatgaattt cagacaaaag
     2881 gagattataa agctgagttc aattcagaca ataataacat cagtctttca agcggcatct
     2941 atttttacac acttgaaacg gcggagttta tcagtacgcg taaaatgatt ttactgaaat
     3001 aatgaaataa taatataagt tttttcattt ctttacctga ctcccgtttg atatttcaga
     3061 acgggagttt ttattaatta ctatgctcca agactgttct aaaaaacaaa attacaaaga
     3121 ttgcctccaa atctcgatga ctcaaaggag ttaattttta actttttaat ctttgagaca
     3181 tggagtcttt gagacaattc caacaccccg gatggatata aaattttcaa aacaataatc
     3241 ataaaaattc aattgtaatt caaaattcag agcaatatat taacattcgt gaaaaattac
     3301 ttttcaaaaa tattcgctgt atatatttta atacttgcca ttataccatg cactgatttt
     3361 atatctcatg gtctttgtga agatactgac aatatacaca ttgagaattc tacagaatct
     3421 gaacatgaag atctgtgcac tccaatgtgc acctgtaatt gctgcaacag cattgttact
     3481 ttgactgaac aattttcact taaaaattac attccgcatt cagaaatact tttgaccgaa
     3541 gtatcacaat acgatgattc attttcgatg aatacatctc cgcctccgaa atcttaattt
     3601 aattttaagt tccacaaaac attattcaac tgacattcaa gatgcagttg ttgttcattt
     3661 tttaaaatta aattatgata aacaaaataa ttgctttttc tataaagcaa aaattagtga
     3721 tcggattttt aatattagta ttaattctgc ttggaatata ttcttccgtg aagcttccca
     3781 tagatgcagt tcctgatatt accaataatc agatccagat cattacatcc agtcctacac
     3841 tgtcagctac agaagtagaa agattcataa catacccggt agaaatagca atgaaaagtc
     3901 ttcctgatat aattgaaatg cgttcgatct caaagtttgg aatatcagta gtgacagtag
     3961 tatttgaaga taatattgat acatattttg cgagaaattt agtttttcag aagcttgtag
     4021 aagctgaaga aaatatgcct gaaggacttg gtaaacctga aatggctccg gtaagtacgg
     4081 gactcggtga gatataccag tatatcgtga gacctgaaaa tcttaatgac accacataca
     4141 gcgacatgga gctgagaacc attcaggact gggtagtaaa aagacaaata ctcgggacag
     4201 aaggtgtagc tgaagtaaac agtttcggcg gctttgagaa acaatatcac atacttgtaa
     4261 atccggataa attaaaaagt tttgatctca ctttacggga agtttttgat gctgtattaa
     4321 ataacaacag taacgtaggc ggcggataca ttgagaaaaa ttctgatcag tattcaattc
     4381 gcggaattgg attaattgaa aaaacagaag atcttgataa tatagttata aaaactattc
     4441 acggaattcc tgtatatctg aatcagatcg cagagataga atttggaaaa ggtcttcggg
     4501 taggtggcgt gacacaggat ggtaacggag aagtagttgc cggaattgtc atgatgctta
     4561 aaggtgcgaa ttcgagggaa gtagcacaga gagttcatga aaaaatttct gaaataaaaa
     4621 caacattgcc agatggtgta acaattgatg aattttataa cagggagaat ttaattgaca
     4681 gtactattga tactgtgaag accaatctta tcgaaggcgg tgtgatcgtc atctttgtac
     4741 tggtgctgct gcttggaaat cttagggcgg gatttattgt agcatctgtg atccctcttt
     4801 caatgctttt tgccatcatc atgatgaata tttttaatgt gtccggaaat ctgatgtctt
     4861 tgggagcaat tgattttgga ctgatcgttg atggtgctgt tattatagtt gaatctgttc
     4921 ttgttgcttt atcagcaaag gtatatttaa ataatgatcc gaaatcaaat atagaattca
     4981 atgatacaat ttattcttca tctaccggaa taataaagtc tgccatcttt ggagtgctga
     5041 ttattatagt agtatatttg ccgatctttg ctttaggcgg tattgaagga aaaatgttta
     5101 agccgatggc atttactgtt ggatttgcac ttgtcggtgc tctcatactt tcgatcacat
     5161 atgtacctat gatgtgttcg cttattctta agaaaaatat gagtgaaaag gaaactatag
     5221 cagacagaat tatgaaaggt ctgaaaaaat tatatacacc atcactggaa tttgctctta
     5281 gaaataaaat cctgactatt gcagcagctt taatatttct gattatcagt ctgatctcat
     5341 ttaccaatct gggtggagaa tttattccaa aacttgatga aggtgacctg gcttatcaga
     5401 tagttcgtat acccggtgta tctttaactg aatctatcaa catcggaaca cagatcgaga
     5461 aaatcctgat ggaaaaattt cctgaaataa aaactgtagt taccaagaca ggtgcgcctg
     5521 agcttgcaac agatccaatg ggacctgaat tcagtgatgt gatagtgatg ctaaagccta
     5581 aagatgaatg gtcttctgct gaaactaaag aagagcttat tgaaaaaata caaaagtcac
     5641 tctcagtaat tccgggtgta ggcttgtctt ttactcagcc gattgaattg agatttaatg
     5701 aactgatagc cggtgccagg ggagacatag cagtcaagat atttggagat gatcttactg
     5761 tactatcaga agaagggaat aaagttgcgt ctattatggg atcagtaaaa ggttcggaag
     5821 atatttcagt tcagcaggta gaaggacttc cgcagcttca gattaagatc aggagagaca
     5881 ggatttcggt ttacggaata aatgtagatg aagtaaataa tgtaattgag acagcactcg
     5941 caggtaagac ttcgggagta gtattcgaag gcgataaaaa gtttgacata gtgctgaagt
     6001 atgatccttc ttatacatcc aatattgaat ccatcagaaa tattttagta tcatcaccgg
     6061 ataatgtgat gatccctctt tctgaacttg ctgacataga tataaaagaa ggaccggcag
     6121 agatcacccg ggacaatggc aaaaggagga tcgtgattca gggaaatgtc agaggcaggg
     6181 atattgaaag ttttgtgaat gaacttaaat ctgatataga tatggaaatg aaattacctc
     6241 ccggatatgt gatcgaatac ggaggacagt ttaaaaatct cgaaagtgca agagagcgtt
     6301 tgatgatcgc tgttcctgtt tctttatttc tgatatttgc tttgctgttt ttaactttta
     6361 attcggtcaa gcagggactt atggtttttt ccggaattcc tttcgctgtc gtaggtggaa
     6421 tatttgcatt ggtcatcaga gatttgcctt tcagtatttc cgcaggcatc ggattcattg
     6481 cgctgtttgg agtagcagtt ctgaatggaa ttgtaatgat cgcatacttt aacaggcttg
     6541 aggatgaagg aatatccaat gttcatgata ggatcgttag cggatccgct tccaggctgc
     6601 gtcctattct gatgacggca ttggttgcat cgctcggatt tattccaatg gctttatcaa
     6661 cgggatctgg tgcagaagtt cagagacctc tcgctacagt tgtcattggt ggtttgatat
     6721 cttctacttt gttgactctg attgtacttc cggtaattta tggaattttc aataaacata
     6781 aaaaacatca aaatcctgaa ttgataaagt ctttgaacta aataagtatc aattttaata
     6841 tgataaaaaa aatcaaagca tataaaatca aatttataga tatgagaaat tttaaattaa
     6901 tcagtataaa caaaatgata aaaattattt tatttacata tttcacatta tttttcagtc
     6961 agggaataat ttcccagact aaaatggata tggataatat aataaaagtt gctgttgaaa
     7021 ataatcaatc tcttaaaact gcccggctga atattgataa agaaaatgca accggcttga
     7081 gatcgttcaa tattcccaaa cctgaaatat ttgtggagta tgaaggcgtt cagggaagtc
     7141 tgagcaatgc tgaaagcaga aagatcggct tcctgcagca gttcgaattt ccaacaaatt
     7201 attttctcag atctgatatg cagaattcac agattagtgt agctgaacag gaattaaatg
     7261 ctaagctaaa tgaacttaaa gccgaattaa aaatgaacta taacaggctg cagttactgt
     7321 atgagctgat caggtcttct gaagaaaatt taaatattta tagtgaattt cttaatacag
     7381 ccgaaaaaaa atatgaagca ggtgaaactt ctaatcttga agtgctgggt gccaaagtaa
     7441 ataagattaa atttcagaat gagattacaa attataaaac ggaaattatt agtgtgcagt
     7501 ctgaactcag aaatctgatg aacgtgaatt atgatgttgt acctacggat gaaatgaatt
     7561 ttaaagaact gaatctaaac agagcatcca ttctaaataa tgccgaaacc aataatccgg
     7621 aaataaaagt agcgggtttt cagaaagaaa aattttcaaa taaaatatca ttatctaaaa
     7681 acgaactcct gcctgacata agttttaaat actttcgtca gaagatcggc gggcaaaatg
     7741 attactgggg catggagatc ggacttggag taccgctatg gttttggtgg gagcaatcgg
     7801 gaaatataaa agaatctaat tatgaatatc agatagctac cagtgaagag ctgagtttaa
     7861 aaaaatcagt ggaaaaccaa actgacatag ctttttcaga atatgaaaac agtttaaggc
     7921 aggtaaagtt ttttaaggaa gaagctttaa gtgaatcaga agaaatactc agacagtcaa
     7981 aaataagtta tgtagaagga gagattggat atgtggaata tctgcaggct ctgaatttag
     8041 catatgaaac catgacacag taccttaatg cagtctatac atacaatcaa tcaattataa
     8101 aattagaaaa attaacagca tcagaattaa aatgaatatt ataaaaaata aattatcgaa
     8161 agtcttaagt atctctatct tgatttcatt taatatgatc ttgatcttag ctatgcaggg
     8221 atgcaacagt aatgaaaata attcatctga aaaagtggac gatcatgaaa atcatgaaga
     8281 tgaaaatgcc ggacataaag atgaggtgtc attaactgag gatcagatca gagtgatgga
     8341 tataaaattt tcaaaattcg aacccagaaa tataaacgga tatataaaag taaacggtga
     8401 gatcatgatg gatcaggata atgaatccag ggtcggaagt ttaatatccg gaagggtaaa
     8461 gaaaattttt gtaaaggaag gttcttatgt tcgtgcaggt cagactcttg ctgtaattga
     8521 aaatcctgat ctgattaatg ttcaggttga ctatattaat gcaaaaaatg aatacgaata
     8581 ttctaagcag gaatatgaca gacaaagaaa attaagcagt gataatatcg gatcaaaaaa
     8641 gaatttcgca gagattgaaa caaattacaa aagagctctt gcaaattaca gatcacttga
     8701 agaaaaatta tcaggttata aaatttccaa aaacagattc gacaatatat atacagacac
     8761 caccgctgac ctgcagagaa attattctgt tacagccccg atctcaggga atattgttgc
     8821 gcggatggta actataggtc agtacattga accttctgtt gatatgttcc aaattgtaaa
     8881 tacttcaaca gtctatgtag atctgagtat attcgaaaaa gatcttggta atgtgaaggt
     8941 tggtcagaaa gttgtaatcg aaaccggttc aaagcctgat gagaattatg aagggaaggt
     9001 ctcatttata aataaaattt ttaatgacag agacaggact gtaaaggtgc gggttgtaat
     9061 aaataacaga tcacaggagc tctatcctta tatgtttgtt tctgcaaaga tctatgtaac
     9121 cggaggagaa gcattgtctg taccggtctc ttccatcgaa accgaaggag agatcaatta
     9181 tatatttctg aaaactggtg aaagaaaagt aattgatgaa catgatgaac acgaaaaaga
     9241 taatgaacat gaacattcag acggtgaaaa agaacataca gaaagtgatc atagtaacga
     9301 aggtcacact gaggaatcag ggattgtatt taaaaaagtc attgtaaata caggaataac
     9361 cgatggggca tttgttgaaa taattcctgt aagtgaaatg aatttaggtg atgaggtggt
     9421 ttccaaaggt acattttatc tgaagtctga gctgtcaaaa ggtgaactcg gtgagcataa
     9481 tcattgaaca cgatctgtgt tcataattct taatggattt ttttatcaat ataatattcg
     9541 attcaatagg aaaaggtttt tttaagttaa ttacctttgg aaaatttccg aataaaaaaa
     9601 cgcctgttat tatagatgtt ttataatgta ttacataggt ggattattat ttttttctat
     9661 agccgtacaa ttaaattaaa catagcttta ctaactcctc ccaacctccc ctttgaaaaa
     9721 aggggaggag ctttaattgt taatttacca gcggctcaac atttacaatt tacgaactcc
     9781 attcccaaat atttttatct ataataaata ttcctatttg agtaatttgt cctttcacta
     9841 aatcaaaatt ttttatccgg ttcattgaat ccgatcttaa gaaatattct cggttttatc
     9901 attctcctga taattgtact tgtctcaatt ggaatactgt ttaataatct tacaaaaaaa
     9961 tcctactatg atgagtcagg gacttttaaa gtaaaaggca ttacaggaaa tgtaaatgtc
    10021 tatacgaccg aactcggcgt gccgcacata tacgcacaga atgaagagga catgtatttt
    10081 acactcggtg tcatgcatgc ccgtgaccgg ctctggcaga tggatcttta cagaagagta
    10141 gccgaaggaa gactttcgga aattatggga aaaaatgttc tcgaatttga tattcttttc
    10201 cgcactttag ccatagataa aaattcacgc tatcaatatg acaaactttc tttcaaatct
    10261 aaatcaattc taaattcata ttccgccggc gtaaattatt ttattgatga aaattcctcg
    10321 cggcttccgc ttgagtttga tattctgaat tataaacccg atcagtggaa acctgaacat
    10381 tccctgatgc ttgtaaaact catggcatgg gagcttaacc tatcatggta caccgacgtt
    10441 atgttcggag agatcgtcaa aaaattcggt gtcgaaaacg caaaatattt ttttcctgat
    10501 tatcctgaag atgcaccgtt catagtaaaa gatgaccagt caaagatcaa aactgattca
    10561 gttaaaaaac caacagtctc tcctgtttct gatttgttta aatcaaaaaa ttatggtgac
    10621 atctctgcac tgggaaaaaa ttatttccaa tcttcattaa atttcagaaa atttttcgga
    10681 acggaaggta ctcatatcgg cagcaactca tgggtcattt cgggaagtaa aacagaaaca
    10741 ggaaaaccta tcctagctaa tgatcctcat ctcgctttgc aggtaccgtc gaaatggtat
    10801 gaggtgagtt tatttaatga acaaaataaa aacagcatct gcggattttc tataccggga
    10861 gtaccgggta ttgctatcgg acataatgac atgatctcat ggggactgac aaatctgatg
    10921 tgcgatgatt ctgattttta tattctcaaa agagattcat ccgtcagcag taaatacatc
    10981 tatagaaata cttccttcgg actcgacagt actgttgaaa gtataaagat caaagatgaa
    11041 aaagatgaac atgtaattac tgtttactca actgtactgg gacctgtagt ttcgaaactt
    11101 gggacaacag gctttttaaa cagacaaaat tttaaaacag aaaatgaaaa cgaattttta
    11161 acttttaaat ggaccggtta tgaatacagt gatgagatcg aaacttttta taaattaaac
    11221 aatgcaggca actggaatga attcagagat gcattaaaaa cttttggtgc acctgcattg
    11281 aattttgtat atgccgacac ttcaggaaat atcggctaca gatctgcagg tcttataccc
    11341 gtaagaaatt ttaccgaaag tgaatatccg ccttacactc ctcaaaattc cggagcactc
    11401 tggaccggat ttgtgccgtt tgaggaaatg cctgaatctg taaatcctga agccggatat
    11461 attgtaactg caaataatag acctaaaaaa gattacaaat attatatctc taatctctat
    11521 gaaccgccat acagagctga acgcatagaa gaacttatca aaagtattcc cctggtcaat
    11581 gaagatgaaa caaaaaaaat tcagcgggat gtgtacagca ttcaggcaaa agaattttgc
    11641 gaatttcttt ttgaagcttt cggtgattca tcagacatta cacctgacat caaatcttat
    11701 ctggatattt taaaacagtg ggattatgaa ttcagacttt ccagtacagg cgcaagtatc
    11761 tttgcaatgt tcgaagtgca gctctataaa aatctttatc agaatgaact tggcagtgat
    11821 ctgtttgaaa attatctata tctgaaaaat gtccccgtca ggaatacttc aaaacttctt
    11881 aagcttacat cttcatttct ttttgaaaat aatgtcatca aaagacagat tatcagaaga
    11941 agtttctatg aagctgtatc tgcactcatt gcaaaatacg gtaatgatcc tgcgggctgg
    12001 agatgggaat tgatgcatga cgtgtatatg aaacatccgc ttgggattgt tcctgaattc
    12061 agctccatgc taaacatagg tccgttcagt atcggcggta acggtacgac cataaataat
    12121 ctcgaataca gtttttcaaa agctcttgct gatcagagat tcgaaagcta tctcggtcct
    12181 tcgatgagaa tgatctccag tcttaattct ataaaaaatt atctgtctat attaccgaca
    12241 ggtcagtccg gtcagccgat caacaggaac tacaacgatc aggcaagact ctggcttaac
    12301 ggagattata aaaaagtaac gacagatttt gctgaactga aaaaagataa tctgaagatg
    12361 ctgagctttg aacccggaga atgatcttat tttatttgaa attatctgaa tatatttgtg
    12421 aaaaattttt catcataata aactgacact ttgaattcca atgatttcca accaatctaa
    12481 atatgagaaa tacagaccaa agaaattcct tggtcagaat tttctaactg atgagaatct
    12541 tgctaagaaa attgtaaact ctcttgatat aaaacctgat gatacagtag tcgagatcgg
    12601 tccgggacaa ggtgtgctga cgaaatatct ggcaaatctg acagatgatt tttttgctgt
    12661 cgagctggac agacaaattg ccggtgaact ttctgaaaag tttgaaaata agatgactgt
    12721 cattcataaa gattttctga aagttgatat tacagaagaa attttttcca aaagaaaaaa
    12781 taaaggcagg ctaaagatca ttggtaacat tccatacaac atcacaacgg aaataatttt
    12841 tcttctaact gaaaacagaa atttgatctc gacagcggtt ttaatgatgc agagagaagt
    12901 ggcgaagcgg ctgacttctc cgcacgggaa taaagattac ggtatacttg ccgtactgat
    12961 ccagttatat tccattccaa aaattttatt taatgtcccg ccggaagtat tttttccaaa
    13021 accccgcgta acatcttcag ttgtaaaatt tgacttccct gagaatattc actcctgtat
    13081 taattatgct ttgcttagta aagtagttaa aaaaagcttt ggtaaaagac ggaagacaat
    13141 gagaaattct cttaaggatt ttttcatgga aaaggaaatt gcgcaggaga atattgattt
    13201 tgattttacc agaagaccgg aaagtctttc gatagatgaa tttctgaaac tgactgaaga
    13261 tattgaaaaa gagatatttg acaacgaaag cagaaagtaa ctctaaggct ccaaaactcg
    13321 aagagaattt tttttaccgt cggaatctcc tgcgactttc cgaaaccccg gcaaatagta
    13381 cggcatgggc agaatacaca aaaaacagtg agactctgct tgaatttaga caaatcgctc
    13441 gcgggcgatt ttttttttta cttgttttag cttactctcg aatgctccag cctgtccgaa
    13501 aacgtaatag tttaaggcaa ctctaagtat cccgattgga ctagtccaat attttgaaag
    13561 aaataagtca ttttgaatca attttactgt ttttaaaata acttatatag gagactcgaa
    13621 tgctcaaagg agattctttt gtaggtttgt ctcttcgagt cttcgagcct tagaggttgt
    13681 tttactgttt tcggacaggc tgtctgccgg gaataactgt tcaccttttc acatcaaaaa
    13741 aactcctcaa tatctcccga cacttatcat caagaatccc gcctgtcaca ttacaattat
    13801 gattaagctg tttattattc gtaatgttca acacgcttcc gcaggctccg cttttattat
    13861 caaatgcacc gaagtaaaga ttctcaatct ttgatagtac tatcgctccg gcgcacatgg
    13921 cacaaggttc aagagtcaca tacatactgc atcccaccag ctgctttgac tgcagatatt
    13981 cagctgcgga agtgatcgca attatctccg catgtgcagt aggatctttc agcgtctcga
    14041 tctgattatg tgcttttgca attatcgtat tttgaaaaac tatcacgcag ccgacaggaa
    14101 tctcctgttc ctcatatgcc gtctccgctt cccggtatgc gtagttcata aatgtgtcgt
    14161 gtccgatcat tttaatatta tattattttt taacagataa tgtgtctttg tctaatgccg
    14221 gattctgatc gctgatcatc tttacagctt cattgatgct gataaattta tagcccggct
    14281 tttttaattc ttctgtcctg ttttcaaatt ccctgaaatc cttttcagaa aaacttacag
    14341 tatagaactg gacttttatt cctttttctg aattcttttt tatctcttca aaaagtttgc
    14401 tgaccttatt ctcttctgtt tttatagctt taaataatgt atcgctgaag actgtgaaat
    14461 tatttttttt aaattccgaa cttatttttt cagcaaactc tttcattcct tttggataat
    14521 aaagtactac acctgagact cccgggaaat tcaccgctgc agttctgact ttttgtctaa
    14581 taacattttc tttcatctca gttctgaaat ctgaagtaat gtcatcattg cttccgatat
    14641 aaaatttcaa gagataattt tttctgttat ctattatcat agactgataa tctgccttat
    14701 catttccaat tggtaaaaaa acagaaaatt tttcaactga ctttataata ttttctgcat
    14761 tctgaaaatc aatatattca atactgtcca gtactatagc cgtttcagaa acattccggc
    14821 taatgtcctt tgagtaaata aaattgagtt tggcatatcc tgtctgcaaa ctatccttca
    14881 cttgaagtat ttctattaaa atatcttttg aacgcgggtc ttccgatgtc tttgaaatga
    14941 atccgttctc ttcgagatag tttgttatat ccagattaag attaatggtc tgaatatcaa
    15001 caggaatacg aattgactga gcaaggataa gacctttatc tccggagata ctaactttat
    15061 tttcaggtac tttgatcttc cattcttctt ttattccaaa tgaataaata attgaatcta
    15121 tatccttttt gaatttttca ggagtaatag ctgctttgtt ctctaatgaa ttttcaggtt
    15181 caactttatc cggatcgtaa tttataaaaa taaaagtaag tattaataat ataactccaa
    15241 gcccagtgat caatattact ttatactttt cggtatttgc catttattga aaatttttat
    15301 cggtacttat tatatagatt gcctcaaaga ctctaagtct ctaagtctct aagtctcaat
    15361 gaagtttaaa tttaattttt taaattcaga gttttgtaga cttttaggta aacccatatt
    15421 catttttata gaatagaatt tctataaata ttgggaaagt ttaaagtata ttataatagg
    15481 ctttgaaata aattaacgat ttatgaaata agcttatcat aaattgtgat aagataaaat
    15541 tcttcaaagt tccctttcta attttcgtca ccaataataa taaaattttt tgcttttaaa
    15601 aatcccgcct ttaatacatt gccgttcaga attcttgaga aattgaaaaa taaaatatcc
    15661 ttgtctgaga aatttgcgaa gcaagttttg agttggatat
//
LOCUS       DAORVQ010000064        15553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1662_length_15553_cov_5.736160, whole genome shotgun sequence.
ACCESSION   DAORVQ010000064 DAORVQ010000000
VERSION     DAORVQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_15553_cov_5.736160"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1357)
                     /locus_tag="PLX80_06895"
     CDS             complement(17..1357)
                     /locus_tag="PLX80_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY20555.1"
                     /translation="MSESTTPIINPPFPVRKKKKKSRKKMFWIISILLIVLIVVAVIM
                     SGKKEELITVQTEKISKRNITQLVTATGKIQSETEVDISAEVSGEIVSLPFKEGEQVK
                     KGALLVKIKQEAFAPQLQQQNAAINVAESNLKIQEVSLRKFELELDRIKQLYDKGLAT
                     SSDMDNAQINVDQIIAQINSNISVINQQRTGLSSIKYDISKTTIYAPIDGTVTQLNNE
                     SGEKVLGTISNQGSNIMTISDLSKMECQVDVGETDVSLINIGDTALIEIDAFPDRIFK
                     GYVYEIANSAQASQLGTQDEVVNFIVKVRIIDNDVELRPGMSCTVDIEVEKKTDVLAV
                     PIQSVTVREEKDFGMGEDNENENLTRESENRQLKKERPDEIIFFVDNSVAKTEKVKTG
                     ISDDSYIEILEGVTDGQEVIKGSFKAISKELEDGKKVKVDNEMKKNNFGSSEEE"
     gene            complement(1395..2744)
                     /locus_tag="PLX80_06900"
     CDS             complement(1395..2744)
                     /locus_tag="PLX80_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY20556.1"
                     /translation="MKKFTFPLFIFLLFTFAGNIYSQEVLNLRDAINKALKNSPTVSN
                     LEGSVEIQKLNTNTAVGNLFPDLNLSANWNRNNTFSEGTVRFENGVPIIIPKQDTWIN
                     SFGIGVNSNITLFDGFSNYAQVDLQEENEKSVKINLDKEKYDIAFRVNSAYFNVLKRE
                     KIIIINEENLTDSRSQLESVKEFMNVGKRTIADVYRQDVQVAQNELALERALNEFDKS
                     QIDLLLAMNSDFTESYKISDPNIVGELTESELQSIMSKYTDLGTLTKRSLSNRYDYKS
                     TLQQIQVSKTQFSIDQKNLYFPTIIGGISYNLNAANIGGLLDTRSFSFGLSLSYPIFQ
                     GNSIKNRSQISEIAIKQNEDNLRIIEKQIQGEVRKAYIDLETQYKQIEILKRNITSAE
                     QDKLLSEESYRVGTGILLDVQTATIRLNNLRIDLINSYYDFLLSEKNLQYFLGELRY"
     gene            2984..3391
                     /locus_tag="PLX80_06905"
     CDS             2984..3391
                     /locus_tag="PLX80_06905"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQY20557.1"
                     /translation="MIYITRREHFSASHILENITLSKEKNTEIFGKCNNFHGHNYYVE
                     VTLAGTPDKDSSYVMDLKKLKKIITEEILDKVDHKFLNELEMFKNIIPTTENMAVIFW
                     EILSKKTDSDNARLYSIRLYETEKNFVEYRGEQ"
     gene            3388..4182
                     /locus_tag="PLX80_06910"
     CDS             3388..4182
                     /locus_tag="PLX80_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQY20558.1"
                     /translation="MSIFSYKYYEKLKQKKYRREIKKFLIEGDNLVFECLESERYRDS
                     IEAVFVRRDYENGTLLKKIYTEIPNVRVEDLTESVFNKLSETVSPQGIIACVRNIETP
                     EINGSFNSDLIIALDNINDPGNLGTIIRTCYWFGVKEILLGNDTVDHLNPKVIRASQG
                     AVFHTDLYRTEDLKESLLEIESKDRHVYLMDVDSEMNISDIGKDTLNKFVLVFGNESN
                     GISREISSENKFKSMKIKGYSECESLNVAVSAGIAIYEFRNALKIS"
     gene            complement(4293..5228)
                     /locus_tag="PLX80_06915"
     CDS             complement(4293..5228)
                     /locus_tag="PLX80_06915"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20559.1"
                     /translation="MKNLFKLLLPLIVIFTSLNFAKAGGDSTYILPTYQLRVTNYTYT
                     PNTFEFDIYIKHTNPNETRFELAGHQYFFEINPALAPNGTTFTYNYVVNQNGDTLSDL
                     PFRFRPRNPSTAGNLLRLATNSLPGATNGLIIDTAGLGMRVLRMRVGLSDTTQNFNNE
                     SFHFFLRDTITTGSFFTKINAYTGLNGTFNTQISYRMFNIVDSSLTNINNSNGNIVNS
                     ELPQTFDLSQNFPNPFNPATKINFALPVQGNVQIRVYDITGREIAKLVDGIKPAGNYS
                     AEFNGANFASGVYFYRIDVTGEKEFKSVKRMVLIK"
     gene            complement(5340..7040)
                     /locus_tag="PLX80_06920"
     CDS             complement(5340..7040)
                     /locus_tag="PLX80_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQY20560.1"
                     /translation="MKKTFRIIVLCIITFFYTNSQSQYSLKIAFPNLAAFDNPIELVH
                     AYDGTNRLFVAQQRGLIYVFNNDPVVSSKKLFINLTGKVSQSGSEKGLLGLAFHPQYE
                     TNRFFYVNYTFDSASQTWTRISRFTASLSNPDTAITSTEYILLTLFQPFTNHNAGKVA
                     FGPDGYFYFGLGDGGSGGDPNGNGQNRATLLGSMIRINVDSAGGGKAYSIPTSNPYYG
                     NSSGYKEEIFAYGIRNPWKFSFDELNGQLWLGDVGQGAREEIDIIESGKNYGWNKMEG
                     FLCYGTCDTTGKGFTRPVWDYPRTVGTTVTGGYVYRGTKLPGLYGKYICADYGNGKLF
                     SLTLNVDTATSQIIHDASFTIPSLGVDQDNEMYVTRLSGTSGFIYKLYNTQAINLNLT
                     AAIEAFYDDNSNSMNISDTVKVYLHQIVSPYAVVDSSTTVLNSSSLSGWNIFNNTPNG
                     TYYIRISHRNGLETWSKTGGIALTIGNDFEYDFTGSAAQSYGSNEILVGSKYCIYSGD
                     VNQDGSIDLSDNGIIENDANLFITGYVPTDLDADGLVDIADLTFADNNSFNFVTKVTP
                     "
     gene            complement(7179..9998)
                     /locus_tag="PLX80_06925"
     CDS             complement(7179..9998)
                     /locus_tag="PLX80_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQY20561.1"
                     /translation="MNRFFIPVFLLSYVIFFTPKVYSQAGSYYNSISTSSASFVTDLE
                     SRIRSPYTRIAYDNYISTNINNFAAIDNGNGTKSVFCVYTGYEHIYSGSFSFGTMSRE
                     HVFAYSWMPTHPSTSNDQYSDQHHLFPTHQNNANGRRSNHPLGIVVNVTYQFLNGKVG
                     TNNLGQTVYEPMNSFKGDVARAMLFMTVRYDGLSGNDWDFNWLNGTRLPSLSEAPQDL
                     NLLLDWCRQDPPDKWEVDRNNYIQSIQQNRNPFTDHPEYTAYINFNDLTKLNPVYATE
                     ATNHFSSFSSVPTSNSINLSWTDASGAQLPDGYLILAYDMNNYFVPVDGSEYVTDTIL
                     SDGTAILNTPHTDPNSHTFNNLISTKTYYFTSYSYIGSGASRNYKINGTVPQTNATVS
                     NPLVAEPTNHITNFAAGTVTQSTAELTWTDAIPGSQAPSGYLLIANNSNTFTNPSDGV
                     TYTDDMILSDGSAVMNLTYAGANNHSFNGLNSNTGYYFRMYPYNGTGTQRNYKTNGSI
                     PQVNAATQPGSQNYSSVLLDNFSKANNNSLGNSLSPFSAVWQETETNNPTSISVSNGY
                     NKSASTTAGRDFAHVDLSSVSGYPSNFTNAGNLLEWAINMRQTRTDPSGFDNNNYGTA
                     FILGKTTSDVTTGEGYAVILGQSGSTDALRLAKFSNGINGNSRFTNVISSGDYANQYL
                     SIRVTFDPSNKVWTLYADNAAGNFPQSDPRNAATLMGSTADSSYTDQNLFFTGTLWNH
                     VTGANDSCIYDEIYIPYNSNTTLDLTLVTEGLYNTMINRLNKSDTMKVYLRSSSAPYE
                     IVDSSKAVIDSVDFTGTFLFSNITTGNYYIAVTHRNSVETWSNQPLNFTQGNPSLYDF
                     TNSASKAFGNNLIQKGSKYCIFSGDVNQDGTVDISDLSQIDNDAYNFTSGYVSTDING
                     DDIVDLTDAAATDNNANNFISKITP"
     gene            complement(10283..13606)
                     /locus_tag="PLX80_06930"
     CDS             complement(10283..13606)
                     /locus_tag="PLX80_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY20562.1"
                     /translation="MKLKLLLLMLSITLTQTAFSTNYYFYKGNKIQLSQRHDKIAVVF
                     NNSISDAMAQNFILPIISPGDSVKRSFSDLYLIKFKERKTDGELKIFTDRLSENSSVK
                     FVTNTYYGTSNSVTQIPYDRIIVKLNISLNKEKLDIMNVQNGCVISGEFEKGVGFILK
                     TVSGNPKNALEMSEIYFKSGIFEYCEPDFIYPEYCLLQSVPNDPFYQQQWNLNNTGQT
                     IQTGSTFFFYGDRPTVNGIPGSSMHVEEAWDFTTGSPSVKMCVIDSGIDSAHIDFQAP
                     GHLLSGYDAFDNTEGSSVDVFNHGTSTAGIMGAVRNNGIGIAGIAPDCKLMSIKIYDI
                     NGNTSSSIIARAFDTARVRGIDVINNSWGGGTPKSIVTDAINDAALNGRGGKGCIIIF
                     ASGNDGHDPPLYPSVLPNVLSVGGSTTHDQMKAPGNGNQFYWGGNYGENEMGDLDIVA
                     PTNVYALMMGNGYEQNFWGTSATAPNAAGVAALVLSVNTGQTRLQVYENLLRGCDKPD
                     NVSYDTTKNYGKWNEYYGYGRVNALNSVRLAAGIDVTPPTIVHKNVPSKDNTYPTLIS
                     TEILDQDGSSVPVSGSNRPVLFFKKKKNGGAWSSYDSVNHITNSGNNYSFQIPSQGWE
                     TEIKYYIRARDNSGNESVFPLHAPNEFWLCYFSICDILSDTKKINGFSGVDYGATVSP
                     AVTFGTFNVLEARVQVYLRHTYLNEEAFSLFSPLLNENNSRKCLFSSNGGDMDDINGA
                     TIFDNAPDKWVNDNPPYLGGSYKPDYTMIGLNGVNAAGNWRILHFDRAISDYAFFDSV
                     FITLYKNSGVKSPSVGLDKSSDSIISFGTVNYPQIYEKQIYIKNTGTSNLTLSSPVFT
                     GSFSTLFTLINTLPTPITPDDSGLITVRLNTAGLSSVSESDAYENAMLNIQTNDPSKG
                     DFNISLQSDNELMTGLKNLNLTLLIEGFYNSFSNTMISDSVLVQLRNINSPYNVIGSE
                     KAVLNDSGKGNFNFSSASNSTPYFIAVKHRNSIETWSAGGVSFISSLAAYNFTNASSK
                     AYGNNLTLINSRYTIYSGDVTQDGIVDLADLSPIDNDAFNFIIGYVPSDINGDTLVDL
                     NDLSITDNNAFNIVTIMRP"
     gene            complement(14010..14996)
                     /locus_tag="PLX80_06935"
     CDS             complement(14010..14996)
                     /locus_tag="PLX80_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HQY20563.1"
                     /translation="MQLSFKLFRTALILFLLVAVNSISKATLYNINVPVNAAQEVPTN
                     TSVGTATLTGTYDDVTNVLNFTFVFSGLTGNTTASHFHGPAAVGVNAGVRIGNTGFQT
                     GVTSGSYSNTFVLTASNETELLTNLWYLNIHTSSFGGGEIRGQVYAVPVTVLKLTTLL
                     EGFYDDGADLSIRDTITVNLREVSSPYNLVESKKVYMKTNGDDTVKYLNATNGTNYYL
                     QLEHRNALETWSGGTIMFAGYSGGYDFSTNDAQAFGSNQVLKGSRYCLYSGDANSDGI
                     VDINDLSDIDNDAFNFAGGYVVTDINGDNFIDLNDLSICDNNAANFVSVIAP"
     gene            complement(15188..>15553)
                     /locus_tag="PLX80_06940"
     CDS             complement(15188..>15553)
                     /locus_tag="PLX80_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20564.1"
                     /translation="DIADLSQIDNDAFNFNSGYLSTDVNGDAFIDLADYALTDNNASN
                     FVGAIVPPAPPPSVDLNEFNDIAPSTIEDPNTGISNNRIERNDDTGDDSKKDENRLDE
                     NYKTGRKISQEEIDKADGK"
BASE COUNT         4929 a         2719 c         2491 g         5414 t
ORIGIN      
        1 aatttattct attaatttat tcttcttctg aacttccgaa attatttttt ttcatttcat
       61 tatccacttt cactttttta ccgtcttcaa gttctttact gatcgccttg aaactacctt
      121 tgatgacttc ctgtccatca gtaactcctt ctaaaatttc tatatatgaa tcatcactga
      181 taccggtttt gactttctcc gtttttgcga cactattatc aacgaaaaat attatttcat
      241 ccggtctttc ttttttaagc tgtctgtttt cggattctct tgtaagattt tcattttcat
      301 tatcctcacc cattccaaaa tctttttctt ctcttacagt aactgactgg atcggaactg
      361 caagaacatc tgttttcttt tcaacttcaa tatcaactgt acagctcata ccaggtctca
      421 gctccacatc attgtcaatt atcctgacct tcactataaa gtttacaact tcatcctgcg
      481 ttccaagctg tgaagcctgt gcagagtttg cgatctcata aacatatcct ttgaatattc
      541 tgtcaggaaa agcatcaatt tctattaaag cagtatcacc aatatttatc agagagacat
      601 ctgtttcacc aacatccacc tgacattcca tttttgaaag atcagaaatg gtcataatat
      661 ttgaaccctg attcgaaata gtacctaaaa ctttttcacc tgattcgtta ttaagctgag
      721 ttacagtccc gtcgatagga gcataaatag tcgtctttga aatatcatac ttgatcgaag
      781 aaagaccagt acgttgctga ttgatcactg aaatatttga atttatctga gcaataatct
      841 gatcgacatt gatctgagca ttatccatat cacttgatgt agccagacct ttgtcatata
      901 attgttttat cctgtcaagc tcaagttcaa atttcctaag tgaaacttcc tgaattttca
      961 aattactttc agcaacattt atagctgcat tttgctgctg aagctgtggt gcaaatgcct
     1021 cctgttttat ctttacaagc aatgctcctt tttttacctg ctcgccttct ttaaaaggaa
     1081 gggaaacaat ttcaccgctg acttcagcgc tgatatcaac ttcagtctcc gattgtatct
     1141 taccagtagc cgtaactaac tgagtaatgt ttctctttga aattttttcc gtctgcactg
     1201 tgataagttc ttcttttttt ccggacatta taacagcaac tacgatcagc acaataagta
     1261 aaatggaaat tatccagaac attttcttcc ggcttttctt tttctttttt cttaccggga
     1321 atggcggatt gataataggg gttgtagatt cagacatatt ttaaagtatt aaatttattt
     1381 attaaaattg attattaata tcttagctca cctaagaaat attgtaaatt cttttctgaa
     1441 agaaggaagt cataatatga atttattaaa tcgatcctca aattattcag tcttattgta
     1501 gcagtctgga catcaagtaa tataccggtt ccgactctgt aactttcctc agaaagaagt
     1561 ttatcctgtt cggctgatgt gatgttcctt ttaagaattt ctatctgttt gtattgtgtt
     1621 tccaggtcaa tgtaggcttt tcttacttca ccctggatct gcttttcaat tattctaagg
     1681 ttgtcttcgt tttgttttat ggcaatttct gagatctgag atctgttttt gatgctgttt
     1741 ccctgaaaga tcgggtagct taaagacaat ccaaaactaa aagatctggt atccagcaga
     1801 cctccaatat tcgctgcatt aagattataa cttatacctc caataattgt agggaaataa
     1861 agatttttct gatctatgga aaattgagtc ttacttacct gaatttgctg caatgtagat
     1921 ttataatcat acctgttact caatgacctt ttagttagtg tacccagatc tgtatatttg
     1981 ctcataattg actgaagttc actttccgta agttcaccca caatgttcgg atctgatatt
     2041 ttatatgatt cagtaaaatc ggaattcatt gcaagaagaa gatctatctg acttttatca
     2101 aactcattca atgctctttc cagtgcaagt tcattctgag ctacctgaac atcctgtctg
     2161 taaacatctg ctatagttct tttaccgaca ttcataaatt cttttacact ttcaagttgt
     2221 gatcgggaat ctgtaagatt ctcttcgtta attataatga tcttttccct tttcagaaca
     2281 ttgaaatagg cactgttcac tctgaatgca atatcatatt tctctttatc aagattaatt
     2341 tttacagatt tttcattttc ctcctgcaga tcgacctgag catagttaga aaatccgtca
     2401 aacaaagtaa tattggagtt aactcctatt ccaaaactgt ttatccatgt atcctgctta
     2461 ggaataatga tcggtacacc attttcaaat ctgactgtac cttcagaaaa agtattgttt
     2521 ctgttccagt ttgctgaaag attcagatca ggaaagagat taccgactgc tgtatttgta
     2581 tttagctttt gaatttcaac gctgccttcg aggttggaaa ctgtcggact attctttaat
     2641 gctttattta ttgcatctct gagatttagg acttcctgcg agtatatatt tcctgcaaat
     2701 gtaaaaagca aaaagataaa taaagggaaa gtaaattttt tcattaatta atttttattc
     2761 tggatttgct tcaaaaataa cgaatggtaa aataataagt ttcatttata cataatatat
     2821 aaaaataatt gtttctacta agctttgact ttaaatttat atcaaaggtt tcattaatta
     2881 agccggcatc aattctttta ttgatttaat taccattggt acttttataa ataagtttca
     2941 ttaaaatatt tataacagta atttataaaa attaaatttt aaaatgatat atattacacg
     3001 cagagaacat ttttctgcat cacacattct tgaaaatatt acattatcta aagaaaagaa
     3061 tacagagata tttggtaaat gtaataattt tcacggacat aattattacg ttgaagtaac
     3121 actggcaggc actcctgata aagacagcag ttacgtaatg gatcttaaaa aactaaaaaa
     3181 aattataact gaagaaattt tagataaagt agatcacaaa tttctaaatg aacttgaaat
     3241 gtttaagaat attataccta ccactgaaaa catggctgta attttctggg aaatactttc
     3301 aaaaaaaaca gactcagata atgcacggtt gtattcaatc cggctttatg aaactgaaaa
     3361 gaattttgtt gaatatcgcg gagagcaatg agcatatttt cttataaata ttatgaaaag
     3421 cttaagcaga aaaagtacag acgcgaaata aaaaaatttc ttattgaagg agacaattta
     3481 gtttttgaat gtcttgaatc tgaacgatac agagattcta tagaagctgt atttgtaaga
     3541 agagattatg aaaatgggac acttcttaag aaaatttata ccgagattcc gaatgtcagg
     3601 gttgaagatc tgacagaaag tgtatttaac aagctttcgg aaactgttag tcctcaggga
     3661 ataatagcat gtgtcagaaa tatagaaaca cctgaaatta atggatcatt taactcagat
     3721 ctgattatcg cacttgataa tattaatgac ccgggaaatt taggaactat tatcagaaca
     3781 tgttactggt ttggtgtaaa agagatcttg cttggaaatg acacggtaga ccatctcaat
     3841 ccaaaagtca taagagcgtc acagggagct gtgttccata ctgatcttta caggacagaa
     3901 gacctcaaag aaagtctgtt ggaaattgaa tcaaaagaca gacatgttta tcttatggat
     3961 gtagattcag aaatgaatat ttcagatatt ggaaaagata cgttaaataa atttgtactg
     4021 gtatttggaa atgaatcaaa tggtatttct agggaaattt catctgagaa taaatttaaa
     4081 agtatgaaga tcaaaggata ttcggaatgt gaatctttaa atgttgcagt ctcagccggt
     4141 attgcaattt atgaatttcg aaatgcttta aaaataagtt gatcatgaaa caagttaaat
     4201 tttaaaaatt acaatatttt tttaaaaaaa tttcacaaaa aaaaagctgt ctctaaaatt
     4261 tcagagacag ctttaaattt tttactgaat atttatttta taagaaccat tctttttaca
     4321 gatttgaatt ctttctcacc tgttacatct attctataga aataaactcc acttgcaaaa
     4381 tttgcaccgt taaattctgc tgagtaattt ccggcaggtt taattccgtc gactaacttt
     4441 gcaatttctc tacctgtgat atcataaact ctgatctgaa catttccctg tacaggcaaa
     4501 gcaaaattaa tctttgttgc agggttgaat ggatttggaa aattctgtga aagatcaaag
     4561 gtttgaggaa gttcactgtt aacaatatta ccgttactgt tatttatatt ggtaagactt
     4621 gaatcaacaa tattaaacat tctgtaactg atctgtgtgt taaatgtacc atttagacct
     4681 gtatatgcat tgatctttgt aaagaatgaa cctgtagtaa ttgtatccct gaggaagaaa
     4741 tgaaaacttt cattgttaaa attttgagtt gtatcagaaa gacccactct cattcttaaa
     4801 actctcattc caagtccggc agtatcgatt attaaaccat tagtagcacc tggtagtgaa
     4861 tttgtagcaa gtcttaaaag atttccagct gtagatggat tcctgggtct gaacctgaaa
     4921 gggagatcac ttaaagtatc tccattttgg ttgactacat aattatatgt gaatgtagta
     4981 ccgtttggtg cgagtgcagg atttatttca aaaaaatact gatgtcccgc taattcaaat
     5041 cttgtttcat tcggattagt atgtttaatg taaatatcaa attcaaaagt attaggagtg
     5101 taagtgtaat ttgttactct taattgataa gttggtaaaa tgtatgtact gtctccaccg
     5161 gctttcgcaa aatttaatga tgtaaaaatt acgattagcg gtagaagtag tttaaagaga
     5221 tttttcattt ttttattaaa tgttattttt taaaaatagt ttatgcaatc taacgtataa
     5281 atttttatta atccacataa atttttagta ttgatttgga ttagaattac cattcaaatt
     5341 tatggtgtta cttttgttac aaaattaaat gaattgttat ctgcaaatgt aagatcagca
     5401 atgtcaacca gtccatctgc atcgagatct gttggaacat atcctgtaat aaataaattt
     5461 gcgtcatttt caataatccc gttatctgat agatcaatcg atccgtcttg atttacatct
     5521 cctgaataaa tacaatattt actgccgaca agtatttcat tacttccata agactgcgca
     5581 gcagaaccgg taaaatcata ttcaaaatca tttccgattg ttaatgcaat tccaccggtt
     5641 ttactccatg tctcaagtcc gtttctgtgt gatattctta tatagtaagt tccgtttgga
     5701 gtattattga aaatgttcca gccacttaaa cttgaagaat tcagtactgt tgtacttgaa
     5761 tccactacag cataaggtga aacaatctga tgtagataga ctttcacagt atcactgata
     5821 ttcattgaat ttgaattatc atcataaaat gcctctattg ctgcagttaa attcagattt
     5881 attgcctgag tattataaag tttatatata aaaccgcttg ttcccgaaag tctggttaca
     5941 tacatttcat tatcctgatc gaccccaaga gaagggatag taaaacttgc atcatgaata
     6001 atttgacttg ttgctgtatc tacatttaat gtcagtgaaa ataatttacc atttccataa
     6061 tccgcgcaaa tatattttcc gtaaagtccc ggaagtttag tgcctctgta aacatatcct
     6121 cctgtaactg ttgtacctac tgttctggga taatcccata ccggtctggt aaaccctttg
     6181 cctgtagtat cacatgtacc atagcataaa aatccttcca tcttattcca tccgtaattt
     6241 tttccgctct ctataatatc aatttcttct ctggcaccct gtcctacatc ccccagccaa
     6301 agctgtccat tgagttcgtc aaaagaaaac ttccatggat tccgaattcc ataagcaaaa
     6361 atttcttctt tatatccgga actgtttcca taatatggat ttgaagttgg aattgaatat
     6421 gcttttccgc cgccggcaga atcaacatta atcctgatca tagatcccag tagagttgct
     6481 ctgttctgtc catttccatt tggatcaccg ccgctgccac cgtcaccaag tccaaaataa
     6541 aaatatccgt caggtccaaa agcaacttta cctgcattgt gatttgtaaa aggctgaaat
     6601 aatgtaagca aaatatactc agtagaagtt attgcagtat caggattgga taaacttgca
     6661 gtaaatcttg atatcctggt ccatgtctga gaggcactgt caaatgtata atttacataa
     6721 aaaaatctgt tggtctcata ctgtggatga aaggccagtc cgagtaatcc cttttcactt
     6781 cctgactgtg aaacttttcc tgtcaggttt atgaaaagct tttttgaact gactaccgga
     6841 tcattattaa aaacatagat cagtcctctc tgctgtgcta caaacaatct gttggtgccg
     6901 tcataagcat gcacaagttc aattggattg tcaaatgctg ctaaatttgg aaatgcaatt
     6961 ttcaaactgt attgcgattg ggaatttgtg taaaaaaatg taatgatgca taatacaatt
     7021 atgcggaaag tttttttcat ggtgtttttt tttaaatgaa taaataataa ttaatttttt
     7081 aaaaaatata ataatttata taatttactt atatatattt tttctttaat tgttgctgat
     7141 ctgtaattac agatattcta actcttttgg gggtttgttt aaggtgtgat cttacttata
     7201 aaattgtttg cattattatc cgttgccgct gcatccgtca gatccacaat atcatcaccg
     7261 ttaatgtcag tacttacata tcctgaagta aaattataag catcattatc tatctgacta
     7321 agatctgaaa tatcaactgt gccgtcctga ttcacatcac cactgaaaat gcaatacttt
     7381 gaaccttttt gtatgagatt atttccgaat gcttttgatg cagaatttgt aaaatcatac
     7441 aatgaaggat taccctgcgt aaagttcaac ggctgattac tccaggtttc tactgaattt
     7501 ctgtgagtga ctgctatata ataatttccg gttgttatat ttgaaaaaag aaatgtacca
     7561 gtaaagtcaa ctgaatctat gactgctttt gatgaatcaa caatttcata aggagctgat
     7621 gaacttctta aatacacttt catagtatca cttttattta atctgttgat cattgtatta
     7681 taaagtcctt ctgttaccaa tgtcagatca agcgtagtat tggaattata cggtatataa
     7741 atttcatcat atatgcatga atcatttgca cctgttacat gattccataa tgttcctgta
     7801 aaaaaaagat tctgatctgt ataactacta tcagctgttg aacccataag agttgcagca
     7861 tttctcggat cagattgtgg gaaatttcct gctgcattat ctgcataaag tgtccatact
     7921 ttatttgaag gatcaaaagt aactctgata ctcagatatt gattcgcata gtcacctgat
     7981 gaaatcacat ttgtaaatct ggaatttccg tttattccat tagaaaattt agcaagtctt
     8041 aaagcatctg tacttccgct ttgaccaagg attacagcat aaccttcacc tgtagttaca
     8101 tctgaggtag ttttcccaag tatgaatgct gttccataat tattattatc aaaacctgac
     8161 ggatcagttc tagtctgacg catatttatc gcccattcta ataagttacc tgcattagta
     8221 aaattcgatg gatatcctga aactgaagaa agatctacat gtgcaaaatc tctgcctgct
     8281 gtagtacttg ctgatttatt atatccatta ctcactgata ttgaagtagg attattagtt
     8341 tctgtttcct gccagacagc agagaaaggt gaaagagaat ttccaagaga attattatta
     8401 gctttactaa aattatctaa aagtacagat gaataatttt gagatccagg ttgtgtcgca
     8461 gcatttacct gcgggatgga tccgttggtt ttataatttc tctgagtacc cgtgccgttg
     8521 tatggataca ttctgaaata ataaccggta ttcgaattca atccgttaaa tgaatgatta
     8581 ttagctcctg cataagttag attcattaca gcacttccgt cagataaaat catatcatct
     8641 gtataagtaa ctccgtctga aggatttgta aatgtattag aattatttgc aatcaacaaa
     8701 tatcctgaag gtgcctgtga tccgggtatt gcgtctgtcc atgtaagctc agcagtagat
     8761 tgcgtcacag tcccggcagc aaagtttgta atatgatttg taggttctgc aaccagtgga
     8821 tttgaaactg tagcatttgt ctgtggtaca gttccgttta ttttgtaatt tctgctagct
     8881 ccgctgccga tataagaata agatgtaaag taataagttt ttgtagaaat cagattatta
     8941 aaagtatgtg aattcggatc agtatgcgga gtatttaaaa tggcagttcc gtctgaaagt
     9001 attgtgtctg taacatattc cgatccgtca accggcacaa aataattatt catgtcatac
     9061 gccaatataa gataaccatc tggaagctgt gcgccggatg catctgtcca gcttagattt
     9121 attgaattac ttgttggcac agatgaaaat gaactgaaat gattagttgc ttctgtagca
     9181 taaacaggat ttaattttgt cagatcatta aaattaatat aagctgtata ttcaggatga
     9241 tcagtaaatg gatttctgtt ttgctgaatg gactgaatat aattatttct gtccacttcc
     9301 catttatcag gtggatcctg tctgcaccag tccagtaaca gattgagatc ctgaggtgct
     9361 tcactgagcg aaggcaatct ggttccgttg agccagttaa aatcccagtc atttccagaa
     9421 agtccgtcat atcttacagt cataaaaagc atggctcttg ctacatctcc tttaaaactg
     9481 ttcataggtt catatactgt ctgaccaaga ttatttgtgc cgacttttcc gttcaggaac
     9541 tgataagtta catttactac tattccaaga ggatgattac ttcttctgcc atttgcatta
     9601 ttctgatggg tcgggaataa atgatgctga tcagaatact gatcgtttga agtagaagga
     9661 tgggtaggca tccagctgta agcaaataca tgctctctgc tcatagttcc gaaagaaaaa
     9721 cttccgctgt aaatatgttc ataacctgtg taaacacaaa atactgactt cgtcccgttt
     9781 ccgttatcta ttgcagcaaa gttgttaata tttgtagaga tataattatc gtaagcgatc
     9841 cttgtataag gtgatcttat tctggattca aggtcagtaa caaaagaagc tgaagatgtg
     9901 gaaattgaat tgtaataaga gcccgcctga gaatatactt tcggtgtaaa aaaaattaca
     9961 taagaaagaa gaaataccgg aatgaaaaat cggttcaaaa atattttcat aaatattaac
    10021 cggatcagaa ttgatgactt agccggagat aaattttgtg tttacaaaga tatactaatg
    10081 caatttttat atcaattgta ttaatgaaca ataaaatacg ggataagaaa aatcctatcc
    10141 cgtatatagt gacagtttga aaactttccc tgatcagact gcctgattta gccccactct
    10201 gaattttaaa attttaaatt ttaaatttta aaatataaaa gtttcgaaat tcctactttt
    10261 gattatcaga tcataatttt aattaaggtc tcattattgt tacaatatta aaagcattgt
    10321 tgtctgtaat tgataagtca tttagatcta ccagtgtgtc tccgtttata tccgaaggta
    10381 catatccgat tataaaatta aatgcatcat tatctatagg agaaagatct gcaagatcta
    10441 ctattccatc ctgcgttaca tctccactgt aaattgtata tctggaattt attaatgtta
    10501 aattgtttcc gtatgccttt gaagatgcat ttgtaaaatt atatgctgcc agtgatgaaa
    10561 taaaacttac tccgcctgcg ctccatgttt caatgctgtt cctgtgtttt actgcaatga
    10621 aataaggagt actgtttgaa gctgatgaaa aattaaagtt accttttcct gaatcattca
    10681 aaacagcttt ttctgaacca attacattgt aaggtgaatt gatatttctc aattgcacta
    10741 atacagaatc agagatcata gtattgctaa agctgttata aaaaccttct attaacaaag
    10801 taagatttaa attttttaaa cctgtcatta attcattatc agactgcaat gaaatattaa
    10861 aatctccttt ggaagggtca tttgtctgaa tattcaacat tgcattttca tatgcatcag
    10921 attctgagac agaacttaat ccggcagtat tcagtctgac agttataagc ccgctgtcat
    10981 caggagtaat tggagtcggc aaagtattta taagtgtaaa caatgttgaa aaagatccgg
    11041 taaatacagg actgcttaat gtaaggttgc ttgtacctgt attttttata taaatttgct
    11101 tttcatatat ctgtggataa ttaacagtac caaatgaaat tatcgaatct gaagatttat
    11161 ctaatccgac tgaaggactt ttcacacctg agtttttata caatgtaata aatacactgt
    11221 caaaaaaagc atagtcactt atcgcccggt caaagtgaag tattctccag ttacctgcag
    11281 cattcactcc gttcaatccg atcattgtat agtcaggttt ataggaaccg cccagatatg
    11341 gaggattatc gtttacccat ttatctggag cattatcaaa aatcgtcgca ccatttatgt
    11401 catccatgtc tccgccgttt gaagagaaca gacatttcct gctgttattt tcattaagca
    11461 atggagagaa taatgaaaat gcttcttcat tcaaataagt atgacgtaaa tatacctgta
    11521 ccctagcttc gagtacattg aatgttccaa atgttaccgc aggcgaaact gttgcaccat
    11581 aatccactcc tgaaaatcca ttgatttttt ttgtatcaga taaaatatca caaatactaa
    11641 aatagcaaag ccagaattca ttcggtgcat gaagcgggaa aacagattca ttccctgaat
    11701 tatcgcgtgc tctaatgtaa tatttaattt ctgtttccca gccttgtgaa ggtatttgaa
    11761 atgaataatt atttcctgaa ttggtaatgt gatttacaga atcataagaa gaccatgcac
    11821 cgccgttttt cttctttttg aaaaagagta caggtctgtt tgatcctgac acaggaactg
    11881 aagatccgtc ctgatctaaa atttcagttg atattaatgt aggatatgta ttatcttttg
    11941 acggaacatt tttatgaaca atagtcggag gagtaacgtc tattcctgct gcaagtctga
    12001 cagaattcaa agcgtttacc cttccatatc cataatactc attccattta ccataatttt
    12061 tagttgtatc ataagaaaca ttatcaggtt tatcacatcc tcttaaaagg ttttcataaa
    12121 cctgcagtct tgtctgacct gtattgacag acaataccaa tgctgcaaca ccggcagcgt
    12181 ttggagctgt ggcagatgtt ccccagaaat tctgttcata tccatttccc atcatcaatg
    12241 catatacatt tgtcggagct acaatatcaa gatcacccat ttcgttttct ccgtaatttc
    12301 cgccccagta aaattgattt ccattgccgg gtgctttcat ctgatcgtga gtagtggaac
    12361 cgcctacgct cagaacattc ggaagtacag acggataaag cggaggatca tgtccgtcat
    12421 ttccggaagc aaatattatt atacatcctt taccacctct gccgtttaat gccgcatcat
    12481 ttatcgcatc tgtaactatg gacttaggag tgccgcctcc ccaactgtta ttgatcacat
    12541 ctattcctct tactctggct gtatcaaatg ctcttgcaat tatacttgac gatgtatttc
    12601 catttatatc atatatttta attgacataa gtttacaatc aggtgcaatt cctgcaattc
    12661 caattccatt attcctgaca gctcccataa ttcctgctgt ggatgtacca tgattgaaaa
    12721 cgtccactga agaaccttct gtattatcaa atgcatcata tcctgaaagt aaatgtccgg
    12781 gtgcctgaaa atctatatgc gcagaatcta tccctgaatc aattacacac atttttactg
    12841 aaggcgatcc ggttgtgaaa tcccaagctt cctctacatg catactggaa ccgggtatcc
    12901 cattcactgt tggtctgtct ccataaaaaa agaatgtact accggtttga attgtctgcc
    12961 cggtattgtt taaattccat tgctgttgat aaaatggatc atttggtaca gattgtaaaa
    13021 gacaatattc aggatatatg aaatcaggtt cacagtattc aaagattcct gatttaaaat
    13081 atatctctga catttctaat gcattttttg gattccctga aactgttttc aaaataaatc
    13141 caacaccttt ttcaaattca cctgatatta cacatccatt ctggacattc attatatcaa
    13201 gcttttcttt atttagtgaa atgttaagtt ttactattat cctgtcataa ggtatttgag
    13261 tgacagaatt agaagtgccg taatatgtat ttgtaacaaa cttcacagat gagttttctg
    13321 ataatctgtc ggtaaatatt tttaattcac catcagtttt tctttctttg aatttgatca
    13381 gataaagatc tgaaaatgat cttttgaccg aatcgcctgg gcttattatt ggcaaaataa
    13441 aattttgagc cattgcatca gatatactgt tgttaaaaac tacagcaatc ttatcatgcc
    13501 tttgagaaag ctgaattttg tttcctttat aaaaataata atttgtggaa aaagctgttt
    13561 gggtgagagt aatcgagagc attaaaagca ataactttaa cttcataata attttttaat
    13621 tataaataaa ttttatattt gcaaattttc aatatgaaat aattcatatt tgaataaatt
    13681 tgctttccaa aagttattgg ggatgtgaca atatacattt tttattaaat ataaataata
    13741 taataaagtt agatattttt tttaatgttt tgtattagat aatctctgta aagatctata
    13801 ctttaataaa tttcactata atatctaaaa tttatattta gtgaattgtt taactatgtt
    13861 tagtttaata attatcaatg aattatattg ataaatcatt taataaaaaa agccggaaaa
    13921 cttaagtctt ccggctttaa caaagaattt tttttaagca ctcagggtca aacgctttac
    13981 gaattctgag aatactgaaa ttagatatac tatggagcta taacagatac aaaatttgca
    14041 gcattgttat cacagatgct cagatcattc agatcaataa aattatctcc gttaatatct
    14101 gtgactacat atccacctgc aaaattaaaa gcatcattat caatatctga aagatcattt
    14161 atatctacta ttccatcgct gtttgcatct ccgctgtaaa gacaatatcg ggatccttta
    14221 agtacctgat tactgccgaa tgcctgtgcg tcattggtcg aaaagtcata gccgcctgaa
    14281 tatcctgcaa acattattgt acctccgctc catgtctcca atgcattcct gtgttctaat
    14341 tgtaaataat aattagtacc atttgttgca ttcaggtatt tgactgtatc atcaccatta
    14401 gttttcatat atactttttt agattcgacc agattgtatg gagaactgac ttctctaaga
    14461 tttacagtta ttgtatctct aatggaaaga tcggcaccgt catcataaaa gccttcaagc
    14521 aaagtagtaa gttttaaaac agtaaccggt acagcataca cctgacctct tatttcaccg
    14581 ccgccgaatg aacttgtatg aatgttcaga taccagagat ttgtcagaag ttctgtttcg
    14641 tttgatgctg tcagtacaaa tgtgttagag taactacccg aagtaacacc agtttgaaat
    14701 ccggtatttc caattcttac cccggcattt actcctacag cagctggtcc atgaaaatgg
    14761 gatgctgttg tatttcctgt aagtcctgaa aatacaaatg tgaaatttaa tacatttgta
    14821 acatcatcgt atgttccggt cagagttgca gttcctacag aagtatttgt cggaacttcc
    14881 tgtgccgcat tgacaggaac atttatgttg tataatgttg cttttgaaat actgtttact
    14941 gctaccagta aaaacaaaat taaagccgtt ctgaataatt taaaagagag ttgcatttta
    15001 acctccttgg ttttttaatt agtaatagtt agggaaaaaa tttattcaaa tatacaattg
    15061 taaatcttcc tttactaatt caaaattaat agatatttag aattcaaaat tcttacattt
    15121 ttttaaataa aaatagaacg gcataaaaat taccgttcta taatttattt aatataaaaa
    15181 ctaaatccta tttcccgtca gctttgtcta tttcttcctg agagatcttt cttccggtct
    15241 tataattttc atccaatcta ttttcgtctt ttttagaatc atcacctgtg tcatcatttc
    15301 tctcaattct gttattacta attccggtat ttggatcttc gattgtcgaa ggcgcaatat
    15361 cattaaattc attcaagtct actgatggcg gcggtgcagg aggaactatt gctccaacaa
    15421 agttagatgc attattatcg gtaagtgcat agtctgcaag atcaataaat gcatctccgt
    15481 ttacgtcagt actgagatat cccgaattaa aattaaatgc atcattatca atttgagaaa
    15541 gatctgctat gtc
//
LOCUS       DAORVQ010000065        15329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1700_length_15329_cov_5.487037, whole genome shotgun sequence.
ACCESSION   DAORVQ010000065 DAORVQ010000000
VERSION     DAORVQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_15329_cov_5.487037"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLX80_06945"
     CDS             <1..308
                     /locus_tag="PLX80_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY20565.1"
                     /translation="FGEFDLAILELGQYDKKWRYIHLLPEEFLTAALELRAKRILPVH
                     SSKFKLANHPWDEPLKLISEYNSNTEKSLNVITPMIGELVNLKDSSQNFSKWWETIK"
     gene            complement(575..1804)
                     /locus_tag="PLX80_06950"
     CDS             complement(575..1804)
                     /locus_tag="PLX80_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20566.1"
                     /translation="MKKNKFLSLTIFISIFTISFFHLSCDNSSGPESVVSGIIHNEFH
                     VPLSNVKVSTQGKTVLTSEDGSFNLSGISIPYDLTVSDSAFNLNTDIYKGLSASYLEL
                     GFNKYPDINYYAYINAELPVEIFQQDIICKVIFSDKEFTNIYSTLLSPNQNAILQVPV
                     FNNVINGKVHVLTYKIDSSHRILSYENYGTSPEIVLNRGNVVNYIFTNDQLSLNPGEK
                     DVECNIEPNNFSSLNSFYLTFSPKNYLYLNSTFSQMTGNNFQVKIPTGIPDAFYSVVN
                     YNVGSTSGNISLVPNTNYIKVNSPCELISPPNNAADVNNSTPFSFSAGEGSGIYEITI
                     FNNVSGANYKIFTTENNFTLNGLEETGNENFSGDNFTWFVSKKGTFSTVNDYAVNKFK
                     IQNPFVSQSQNGFFTFK"
     gene            2163..2984
                     /gene="rplB"
                     /locus_tag="PLX80_06955"
     CDS             2163..2984
                     /gene="rplB"
                     /locus_tag="PLX80_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQY20567.1"
                     /translation="MALRKLKPMTPATRYYSISSFDEITKSTPEKSLLRPLKKSGGRN
                     NLGRITSRHRGGGHKRRYRLIDFKRNGTGDFEILAIEYDPNRTARIALVADEKGVKSY
                     IIAPDGIKVGEKIVNSNEAEVKSGNALILKVIPVGTFIHNIEMKPGKGAQIARSAGTS
                     AQIIAKDEKYSQVKLPSGEVRLINNDCKATIGIVSNTDHENISIGKAGRSRWMGRRPH
                     VRGVAMNPVDHPMGGGEGKTSGGGHPVSPWGLPAKGYKTRKKKNISNKFIVKKRK"
     gene            2988..3275
                     /gene="rpsS"
                     /locus_tag="PLX80_06960"
     CDS             2988..3275
                     /gene="rpsS"
                     /locus_tag="PLX80_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQY20568.1"
                     /translation="MSRSLKKGPNLDYKLLDKVDKLNSQNQKKVIKTWSRACTITPDF
                     VGHTFAVHNGNKFIPVYVSENMVGHKLGEFSHTRTFRAHSGQRKDEKAGGR"
     gene            3276..3626
                     /gene="rplV"
                     /locus_tag="PLX80_06965"
     CDS             3276..3626
                     /gene="rplV"
                     /locus_tag="PLX80_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQY20569.1"
                     /translation="MEARALKRHIGTSPRKMRLVVDLIRNKRVDDAVNLLMFTNKSAA
                     KVVEMTVRSAYSNLVNKLDSGRLSQGDVIIKEAYVNEGPSMKRILPAPQGRAFRIQKR
                     SAHLTIVVEQAEEK"
     gene            3691..4305
                     /gene="rpsC"
                     /locus_tag="PLX80_06970"
                     /pseudo
     CDS             3691..4305
                     /gene="rpsC"
                     /locus_tag="PLX80_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            4369..4779
                     /gene="rplP"
                     /locus_tag="PLX80_06975"
     CDS             4369..4779
                     /gene="rplP"
                     /locus_tag="PLX80_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQY20570.1"
                     /translation="MLMPKRVKFRKAQRGHLRGNAGRGFTMNFGTYGLKSMEPGWITD
                     RQIEAARIALTRYMKRDGRVYIRIFPDKPVSKKPAETRMGKGKGAPEFWVAVIRPGRI
                     MFEIDGVSRAIAEEAFRLASHKLPLKTKFIARID"
     gene            4831..5031
                     /gene="rpmC"
                     /locus_tag="PLX80_06980"
     CDS             4831..5031
                     /gene="rpmC"
                     /locus_tag="PLX80_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQY20571.1"
                     /translation="MKINQVREMTDEDLKIALKDNHEALQNYRFQLATGQLENYKSLS
                     ATKKDIARIHTIIKEKQIKAKS"
     gene            5303..5545
                     /gene="rpsQ"
                     /locus_tag="PLX80_06985"
                     /pseudo
     CDS             5303..5545
                     /gene="rpsQ"
                     /locus_tag="PLX80_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            5576..5944
                     /gene="rplN"
                     /locus_tag="PLX80_06990"
     CDS             5576..5944
                     /gene="rplN"
                     /locus_tag="PLX80_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQY20572.1"
                     /translation="MIQEETNLNVADNSGAKSVRCIRVLGGSDRRYAGVGDVIVVTVK
                     SAIPGGTVKKGSVSRAVIVRTAKETRRNDGTYIKFDENAAVLINNNNEPMGTRIFGPV
                     ARELRDKQFMKIISLAPEVI"
     gene            5947..6306
                     /gene="rplX"
                     /locus_tag="PLX80_06995"
     CDS             5947..6306
                     /gene="rplX"
                     /locus_tag="PLX80_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQY20573.1"
                     /translation="MADKRKTSKTRLKKNDTVKVISGNSRGAVGKVLFINSEKGSLIV
                     EGVNIVHRHTKPNQKNPQGGIVKREAPINLSNVMLMCPKTNQPTRIGMQLINEDKKSS
                     KKTKMRFSKKAQEIIIS"
     gene            6314..6967
                     /gene="rplE"
                     /locus_tag="PLX80_07000"
     CDS             6314..6967
                     /gene="rplE"
                     /locus_tag="PLX80_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQY20574.1"
                     /translation="MAKTKQDRKKETDGAETEGKDSKSKEGFKEEKVDINLLHTYRDN
                     IVPELKKKFNYKNVMQVPKLEKININVGVGAATTDAKLIETTVKELEAITGQKPTVVK
                     AKKSVSNFKLREGVDIGVKLTLRNARMYEFLERFIRISIPRVRDFRGLSDKSFDGRGN
                     YSLGIKEQIIFPEINVDNVNRISGMDITFVTSANTDEESLALLKAFGLPFIKRQTTN"
     gene            6984..7253
                     /gene="rpsN"
                     /locus_tag="PLX80_07005"
     CDS             6984..7253
                     /gene="rpsN"
                     /locus_tag="PLX80_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQY20575.1"
                     /translation="MARKALIAKQKRREYLVEKYAKIRKELKDNGDYEGLQKLPRNSS
                     PSRLNNRCNVTGRVRGYYRKFGMSRLVFRELALQGKIPGVIKSSW"
     gene            7280..7678
                     /gene="rpsH"
                     /locus_tag="PLX80_07010"
     CDS             7280..7678
                     /gene="rpsH"
                     /locus_tag="PLX80_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006729423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQY20576.1"
                     /translation="MSMTDPIADYLTRVRNAIKAGKKTVDMPASKFREAISVILKKSS
                     LIGDFTTVETDDKFKMLTIKLKYNDGESVILGLKRISRPGIRRYVGHEKLPRVRNGLG
                     IAIISTSKGLLTDKEARSQKVGGEVVCHIW"
     gene            7707..8245
                     /gene="rplF"
                     /locus_tag="PLX80_07015"
                     /pseudo
     CDS             7707..8245
                     /gene="rplF"
                     /locus_tag="PLX80_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
     gene            8268..8639
                     /gene="rplR"
                     /locus_tag="PLX80_07020"
     CDS             8268..8639
                     /gene="rplR"
                     /locus_tag="PLX80_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQY20577.1"
                     /translation="MNQIEKSRKRKERIRFQIRKKLRGTSEVPRLVVYRSLDHIYAQL
                     IDDVNSKTILTVSTKSKDILEKLSGVKGKVEKSKLIGKLTAEKAVSNSISSVVFDRNG
                     YLYHGRVKALAEGAREGGLKF"
     gene            8696..8766
                     /locus_tag="PLX80_07025"
     tRNA            8696..8766
                     /locus_tag="PLX80_07025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8728..8730,aa:Gln,seq:ttg)
     gene            8808..9320
                     /gene="rpsE"
                     /locus_tag="PLX80_07030"
     CDS             8808..9320
                     /gene="rpsE"
                     /locus_tag="PLX80_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQY20578.1"
                     /translation="MKKKKNYKAGELELKDKMVHIGRVAKVVKGGRRFSFSSVSVVGD
                     GNGHVGIGLGKANEVTDSIRKSVEDAKKNVIEVPIIKGTIPHEIIGKYGAAKVMLKPA
                     SPGTGIIAGGGVRAVLESAGVQDVLTKLQGSSNPHNVVKATIRALLLLNDAKKVADRR
                     GITVQELFAN"
     gene            9336..9518
                     /gene="rpmD"
                     /locus_tag="PLX80_07035"
     CDS             9336..9518
                     /gene="rpmD"
                     /locus_tag="PLX80_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQY20579.1"
                     /translation="MLKITQTKSIIGRLKKQKLTIEALGLGRPNYFTLKKDTPQIRGM
                     INVVKHLVKVEENYKQ"
     gene            9614..10072
                     /gene="rplO"
                     /locus_tag="PLX80_07040"
     CDS             9614..10072
                     /gene="rplO"
                     /locus_tag="PLX80_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQY20580.1"
                     /translation="MDILSNLKYAEGSRKKRKRVGRGQGSGHGGTSTRGHKGQRSRSG
                     AKFRAWFEGGQMPLQRRVPKFGFKNINRVEYSVMNLDDIQKLIDDKKILETTIDKEYL
                     LKNKFLKGKNKPLKILGNGEITTKLDITADAFSKTAIEKIEKAGGKAVKL"
     gene            10069..11403
                     /gene="secY"
                     /locus_tag="PLX80_07045"
     CDS             10069..11403
                     /gene="secY"
                     /locus_tag="PLX80_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQY20581.1"
                     /translation="MSGFFESFRNIFKIEELRNRLLFTLAILVVVRIGAHITLPGIDA
                     SLLAEASKNQDSNTLFGLYDMFVGGAFQNAAIFALGIMPYISASIIIQLLGAVFPYFQ
                     KLQKEGEDGRKKINQLTRFGTIPVAALQAWGVSVQLGSRTVGDLSVISPEISSAFFTI
                     STVILLTGGTVFMMWLGEQITERGIGNGISLIIFIGIIDKFPYSVFDEYQIVSSGARN
                     ILVEIIFIIGFLLIIAGVIAVTVATRKIPVQYAKRVVGRKVFGGVTQYIPMKVNSAGV
                     MPLIFAQSIMFVPSTVLSFFPNSEFMQGVAKYFDYTSWFYSLVYGLLIVFFTYFYTAI
                     AFNPKDVSDNMKKQGGFIPGVRPGKPTSDYIDNILTKITLPGAIFLAIIAIIPTFLMK
                     LGVTTGLSQFFGGTSLLIIVGVALDTLQQIESHLLMRHYDGFMKSGKLKSRR"
     gene            11413..12171
                     /gene="map"
                     /locus_tag="PLX80_07050"
     CDS             11413..12171
                     /gene="map"
                     /locus_tag="PLX80_07050"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQY20582.1"
                     /translation="MSLIKTEKEIDLIRESSKIVAGVFEHLKDHIKEGITTAEIDNLV
                     EDFILSKDALPAFKGYRVGKRVFPASSCISVNDEVVHGIPGNRKLESGDVVSVDVGVK
                     KNGYFGDSAYTYKVGETSAKINKLLKITEESLYKGIEMAIDGNEVNDIAVAIQNYVEG
                     SGYGVVRELVGHGIGKNLHEEPSVPNFYSPGSKQRLFEGMVLAIEPMVNYGTYGVKQD
                     KDGWTIRTQDGEPSAHFEHTVLVKKGKAEILTKC"
     gene            12187..12405
                     /gene="infA"
                     /locus_tag="PLX80_07055"
     CDS             12187..12405
                     /gene="infA"
                     /locus_tag="PLX80_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQY20583.1"
                     /translation="MAKQDAIKVDGIISEILPNTNFKVKLENGHEILAHISGKMRMNY
                     IRILQGDKVTVELSPYDLTKGRITYRYK"
     gene            12457..12570
                     /gene="rpmJ"
                     /locus_tag="PLX80_07060"
     CDS             12457..12570
                     /gene="rpmJ"
                     /locus_tag="PLX80_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQY20584.1"
                     /translation="MKVRSSVKKMCSKCKVIRRKGVVRVICTNPKHKQRQG"
     gene            12607..12990
                     /gene="rpsM"
                     /locus_tag="PLX80_07065"
     CDS             12607..12990
                     /gene="rpsM"
                     /locus_tag="PLX80_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQY20585.1"
                     /translation="MARIAGVDLPKNKRVVIGLTSIFGIGKATAQKILAKTGIDEDRK
                     VSALSDDEVNQIRAEIGTETKVEGSLKSEIAMNIKRLTEIGSYRGRRHRRGLPVRGQR
                     TKTNARTRKGKRKTVAGKKKAAAKK"
     gene            13056..13442
                     /gene="rpsK"
                     /locus_tag="PLX80_07070"
     CDS             13056..13442
                     /gene="rpsK"
                     /locus_tag="PLX80_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQY20586.1"
                     /translation="MAKNFKKTKKKANVEANGVAHIKATFNNVIVTLTDLYGNTVSWA
                     SSGKMGFKGSKKNTPFAAQLTAESAAKGAMDLGMKKVEVLIKGVGSGRDAAVRSLNTA
                     GLEVTSIKDITPLPHNGCRPPKKRRM"
     gene            13491..14120
                     /gene="rpsD"
                     /locus_tag="PLX80_07075"
     CDS             13491..14120
                     /gene="rpsD"
                     /locus_tag="PLX80_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQY20587.1"
                     /translation="MARYTGANCKLCRREKQKLFLKGTKCFSDKCPIESRNYPPGQHG
                     NSRRTRMTDYAVQLREKQKVRKTYGVLENQFRRYFEAANKKTGVTGDNLVKLLERRLD
                     NTVYRLGLAPSRKSARQLILHRHFTINGKVVNIPSFLLKSGDLIQVREKSKKLDIIHD
                     SMKRVKDNMLPSWLNLDKANLQGVFMKEPERSDVQFIGNEQLIVELYSK"
     gene            14151..15152
                     /locus_tag="PLX80_07080"
     CDS             14151..15152
                     /locus_tag="PLX80_07080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQY20588.1"
                     /translation="MKINNFQMPESIIKEESTYTDTFGRFVMQPLERGYGVTLGNSLR
                     RILISSLPGSAIIAIRVNDAPHEFTTLKGVVEDLSEIILNLKEVRFKDITNKSSKIEF
                     NLKGQQIFTAQNIQDSTADFEVLNPEKHIATLNKDANLNIEIRMGSGFGYVPSDDNKQ
                     ANLPLEFIYVDSIFSPVVKANYVMENTRVGKRTDYEKLTLEITTDGSINPEDALVNSA
                     RLMQEHIDMFLNLNPENVKKMEEPVRTEKDEEHEMIKKILLMPVDELDLSVRSQNCLR
                     SANIKTISDLVSKNEGEMLHFRNFGRKSLAELGELIENFGLTFGMDVDKYLKEETPV"
     gene            15187..>15329
                     /locus_tag="PLX80_07085"
     CDS             15187..>15329
                     /locus_tag="PLX80_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HQY20589.1"
                     /translation="MVHRRKGRKFNRTASHRRALLANLSIALILNKRIKTTEAKAKEL
                     RRF"
BASE COUNT         5952 a         2171 c         3157 g         4049 t
ORIGIN      
        1 cttttggaga atttgatctg gcaatacttg agcttggcca gtatgataaa aaatggagat
       61 atattcattt gcttcctgaa gaatttctta ctgcagcttt agagctaagg gctaaacgaa
      121 tactacctgt acattcttca aaattcaaac tggctaatca tccctgggac gaacctttaa
      181 aattaatttc tgaatataat tcaaatacag agaaaagttt aaatgttatt actccaatga
      241 taggcgaact ggtaaattta aaagacagca gtcagaattt ttcaaagtgg tgggaaacaa
      301 ttaaatagat ttactgccgt cagtaaacgg atatcggaaa tcagaagtat caactacgga
      361 gacggggtca tctgaacaat ttactattga taaagatatt attataaaaa gaaaaatata
      421 tattgttttg aacttcactt tatacataag gatttttttt aaaattgaaa aaaatgaact
      481 caatattttt aaaaattaaa caaatcaaat tcattattta ttttgaatct ttaatttttc
      541 aaacttttta aagctccgta cacggctcag ataactattt aaaagtaaaa aacccatttt
      601 gactttgtga aacaaaggga ttttgaattt tgaatttgtt tactgcataa tcattaacag
      661 tactgaatgt tcctttcttt gatacaaacc aggtgaagtt atcaccactg aagttttcat
      721 ttcctgtttc ctcaagccca tttagtgtaa aattattttc agtagtgaat attttgtaat
      781 ttgctccgct gacattatta aatatagtaa tttcatatat cccgctgcct tctcccgcgc
      841 tgaatgaaaa cggtgtagaa ttatttacat ccgcagcatt atttggcgga gatataagtt
      901 cacacggact gtttactttt atataatttg tatttgggac taaagaaatg ttaccactgg
      961 tactgccaac attataattg acaacagaat aaaaagcatc aggaattccg gttggaattt
     1021 taacctgaaa attattacct gtcatttgtg aaaaagttga gttaagataa agataatttt
     1081 taggtgaaaa agttaaataa aaactattca atgaactgaa attattaggt tctatattac
     1141 attcaacatc cttttcaccc gggttcaaag ataactgatc atttgtaaat atataattta
     1201 caacattacc tctgttaagt actatttcag gactggtacc ataattttca taagaaagaa
     1261 ttctgtgact ggaatcaatt ttataagtca aaacatgtac tttaccatta atgacattat
     1321 taaaaacagg tacttgtaaa atggcattct gattaggaga aagcagagtg ctgtaaatgt
     1381 ttgtaaattc tttatcggaa aaaataactt tgcagattat atcctgctga aatatttcaa
     1441 ctggtaattc agcatttata taagcataat aattaatgtc cggatattta ttaaatccaa
     1501 gttctaaata tgatgcactt aaacctttat aaatatctgt attgagatta aaagcagaat
     1561 cagagactgt cagatcatac ggtattgata ttccactaag attaaaactt ccgtcttccg
     1621 aagtcaggac agtcttaccc tgcgtggaga cttttacatt tgataaaggt acatgaaatt
     1681 cattatgaat tattcctgag acgacggatt ccggaccgga tgaattatca cagctaaggt
     1741 gaaagaaaga aattgtgaaa attgaaatga atattgtgag tgataaaaat ttgttctttt
     1801 tcataaaata taaaattaat tattttaata aaaattaatg ttggattttt taaaaaaaat
     1861 ataaaatcaa tgttaggaat aatttttttt taaaaaactc aacagacaat taataaagca
     1921 aagttattta atcttaaact gaaatacaac attaaatcag attaacactg aatccaaatt
     1981 tcttttatca aaagcaatgt gtaaaatctt atcttaaata catcccggga taaagaaaaa
     2041 gtgagggtgt ccataatttc ctgtattgac ttttctaacc attttccgta atttgaagtt
     2101 ttccccaaaa aggaacttta ttgaattttg tctaaaattc agaattttta tataatatta
     2161 agatggcact tagaaaacta aaaccaatga cgcccgcgac gagatactat agtatctcat
     2221 catttgatga aattacaaaa tccacaccgg agaaatctct gctaaggcct ttgaaaaaat
     2281 caggcggcag aaacaatctt ggaaggatca catcaagaca cagaggcggc ggtcacaaga
     2341 gaagatacag actaattgat tttaaaagaa acggaaccgg tgattttgaa atactggcta
     2401 tagaatacga tccgaacaga actgcaagga tcgcacttgt cgcagatgaa aagggagtaa
     2461 agagttatat catagcacct gacggaatta aggtcgggga aaagattgta aattcaaatg
     2521 aagcagaagt caaatccgga aacgcactta tcttaaaagt aataccggta ggaacattca
     2581 tacataatat agagatgaaa cccggcaaag gtgctcagat cgcaaggagc gcagggacat
     2641 cagcgcagat aattgctaag gatgaaaaat attcacaagt gaaacttcct tcgggagaag
     2701 taagacttat caataatgac tgcaaggcaa caataggaat tgtaagtaat acagatcacg
     2761 agaatataag tataggaaaa gcaggaagat caagatggat gggaagaaga ccacatgtaa
     2821 gaggagtagc tatgaatcct gtagatcatc cgatgggcgg cggtgaaggt aagacttcag
     2881 gcggcggaca tccggtaagt ccatggggat tacctgcaaa aggttataag acgagaaaaa
     2941 agaaaaatat ttcaaataag tttatagtta agaaaaggaa ataattaatg tcaagatcgc
     3001 tcaaaaaagg tccaaatctg gactacaagc ttctggataa agtggataag ttaaattcac
     3061 aaaatcagaa aaaagtaata aagacctggt caagagcatg taccattact cctgattttg
     3121 taggtcatac ttttgcagta cataatggaa ataaattcat acctgtatat gtgtcagaaa
     3181 atatggtagg tcataaactc ggagagtttt cacataccag gacattcaga gctcattcag
     3241 gacaaagaaa agatgaaaag gcagggggta gataaatgga agccagagca ttaaaaagac
     3301 atataggaac atcaccgaga aagatgagac ttgtagtaga tcttatcaga aataaaagag
     3361 ttgacgatgc agtgaattta cttatgttca ctaataaaag cgcagcgaaa gttgtggaaa
     3421 tgactgtaag gtcagcatac agcaatcttg ttaacaagct tgattcaggc agactgagtc
     3481 agggcgatgt gataattaaa gaagcttatg taaatgaagg tccgtcaatg aagagaatat
     3541 tgccggctcc tcagggaaga gcttttagaa ttcagaaaag atctgcacac ttgactatag
     3601 tagtcgagca ggcagaagag aaataatatc tattataaaa aattataaac aactataaat
     3661 tataatcttc aggattatta aggagtattt ttgggacaaa agacacaccc cgtcggattt
     3721 agattaggag ttatcaatac ctgggattca aactggtatg atgacaaaag ttttgcaaag
     3781 aaactcatgg aagattccgt gatcaggtca tatataaaga agagacttga gaaagccgga
     3841 attgcgaaaa ttgccattga gagaactttg aaaaagataa ctctgacgat acatacgtcc
     3901 cgtcccggat ttgttatcgg aaaaagcggt aaagagatca cacagcttga aagtgagatc
     3961 aagaagatca caggaaaaga agttaagatc aatgtagctg agatcaagaa acctgaactg
     4021 aatgctacat tagtagcgga aaacattgca ggtcagcttg caaacagagt atcattccga
     4081 agagcaatga aatcagcgat tacaaatact atgagaatgg gtgcagaggg aattaagata
     4141 atgtgcggcg gaagattagg cggagcggag atcgcgagat ctgagcagta taaagaagga
     4201 aggattccat tgcagacaat tagagcggac attgattatg catcagtaac atcacatacg
     4261 atttacggag ctattggagt gaaagtatgg atatgcagag gtgaaaaatt gtcaagaaat
     4321 taattaagaa actaaatcag aaattaaaaa tataccggag attttattat gttaatgccc
     4381 aaaagggtta aattcagaaa agcgcagagg ggacatttaa gaggaaatgc aggcagagga
     4441 tttactatga atttcggaac atacggatta aagtcaatgg agccgggctg gatcacagac
     4501 agacagatcg aggctgcaag gatcgctttg acaagataca tgaaaagaga cggaagagtt
     4561 tacatcagaa tattcccgga taagccagtt tcaaagaagc cggcggaaac aaggatgggt
     4621 aaaggaaaag gcgcaccgga attttgggtt gcagtaatca gaccgggcag aataatgttt
     4681 gaaattgatg gtgtatccag agctattgca gaagaggcat tcagattagc ttctcacaag
     4741 cttccgctta agacgaaatt tatagcaagg atagattaag taagtttgga agtaaaataa
     4801 attattaaga ttaagttcta aaaaaataaa atgaaaatca atcaagtaag agaaatgaca
     4861 gatgaagatc ttaagattgc gcttaaagac aatcacgaag cacttcagaa ttacagattt
     4921 cagttagcta ccggacaact ggaaaattac aaatcattgt cagcaacgaa aaaagacatt
     4981 gcaaggatcc atacaattat caaagaaaaa cagatcaaag caaaatctta aatcaattat
     5041 aaagcttaaa taacaataac agatgtctga agatacaaaa gaagaagtca acgaaacacc
     5101 ggaaaacaca cctgaagaaa ctacatcaga tgctcctgca gaaaattcat ctgaggatgc
     5161 agtaaatact ccggcagaaa ctcctgcagt aaactcaact gaagatgcag taaatactcc
     5221 ggcagaaact cctgtggaga cagctgaagt aaaatcagaa gttaaaactg aagaagtaaa
     5281 aaagaagact tcccattcag gattaagaaa aacaagagta tgcttagtcc ttagtaataa
     5341 aatggataag acaataatcg tcagtgatga gagaaggatc cagcatccga tatataagaa
     5401 gttttataaa aagactacga aatttgcagc gcatgatccg gaaaataaat gtgatatcgg
     5461 agataaagta aaaataatgg aaaccagacc tttgagtaaa acaaagagat ggagacttgt
     5521 agaaatagta gaaaaagtta aataacacaa ttttatataa aatagattat caataatgat
     5581 acaggaagaa accaatttaa acgtcgctga taactccgga gccaaatcag taagatgcat
     5641 tagagttctc ggaggatctg acagaagata cgcaggtgtc ggtgatgtaa tagtggttac
     5701 ggtaaagtca gcaataccgg gtggaacagt aaagaaaggg tcagtttcaa gagcagtaat
     5761 agtaagaact gcaaaagaga ccagaagaaa cgacggaaca tatattaagt ttgatgaaaa
     5821 tgcagcagta ctgatcaata ataacaatga accgatggga acaaggatct ttggaccagt
     5881 agcaagagag ttaagagata aacaattcat gaaaattata tcattagcac cggaggttat
     5941 ttaaaaatgg cagataaaag aaaaacatca aaaacaagat tgaaaaagaa tgacactgta
     6001 aaggtcattt ccggcaactc aagaggtgca gtcggtaaag ttctatttat aaatagtgaa
     6061 aaaggatctt tgatagtgga aggtgtgaac attgttcaca gacatacaaa accaaatcaa
     6121 aaaaatccac agggcggcat agtaaaaaga gaagctccga ttaatttatc aaatgtaatg
     6181 ctgatgtgtc caaagacaaa ccagcctacg agaattggaa tgcagttaat aaatgaagat
     6241 aaaaaatctt caaaaaagac taagatgaga ttcagcaaaa aagctcagga aataattatc
     6301 agttaaatca taaatggcta aaactaaaca agacagaaaa aaagaaaccg acggagcaga
     6361 gacagaagga aaagactcta aatccaaaga aggttttaaa gaagaaaaag ttgatattaa
     6421 tcttcttcat acatacaggg ataatattgt acctgaattg aaaaagaagt ttaattataa
     6481 aaatgtaatg caggtgccaa agctcgaaaa gatcaatata aatgtcgggg taggtgcagc
     6541 aacaacagac gctaaactta tagaaacaac tgtaaaagag cttgaagcaa tcacgggtca
     6601 aaagcctaca gtagtaaaag caaaaaagtc tgtatcgaat tttaagctca gagaaggcgt
     6661 ggatattgga gtaaagctaa cgctgagaaa tgcaagaatg tatgaattcc ttgaaaggtt
     6721 catcagaatt tctattccaa gggtaagaga tttcagaggt ctatcggata agagttttga
     6781 cggcagagga aattacagtt tgggtattaa agaacagatc atatttcctg aaataaatgt
     6841 tgacaatgtg aacaggatct caggaatgga tattactttt gttacttctg caaatacaga
     6901 tgaagaatca ttagcgttgc ttaaagcatt tggacttcca tttataaaaa ggcaaacaac
     6961 taattaatta agattataga ataatggcta gaaaagcatt aatagcaaaa caaaaaagaa
     7021 gagagtatct ggtagaaaaa tatgctaaga taagaaagga actgaaagac aacggtgatt
     7081 atgaaggtct tcagaaactt ccgagaaaca gcagtccgag cagacttaac aacaggtgta
     7141 atgtaaccgg cagagtaaga ggttattaca gaaaattcgg aatgtcaaga ttagtattca
     7201 gagagttagc attacaggga aagattcccg gtgtaattaa atcaagttgg taaattaaaa
     7261 gattcaggag aattataaaa tgtcaatgac agatcccatt gcggattatt taacgagagt
     7321 aagaaacgca attaaagccg gtaagaaaac ggtagatatg ccagcttcga aattcagaga
     7381 agcgatcagt gtaattttga agaaatcatc acttattggt gattttacta ctgtcgaaac
     7441 tgatgataaa tttaaaatgc tgacgatcaa actgaaatat aatgacggtg aaagtgtgat
     7501 acttggatta aagaggatca gcagacccgg tataagaaga tatgtcggac atgaaaaatt
     7561 gccgagagta agaaacggat tgggtatagc tataatttca acatcaaaag gattattaac
     7621 agataaggaa gccagaagtc aaaaagttgg cggagaagtt gtctgtcata tttggtaaat
     7681 aatttaaaag tataactgta aaagaaatga gcagaatagg taaaaaaccg attgaacttt
     7741 cagataaagt aaaagtcaca caaaaaggtc agctaataga agttaccgga cctaacggaa
     7801 ctcttaacct tgaattgaca ggcggagtaa gtttagacat taaagataaa gaagttacac
     7861 ttcaaagagc agatgacagt aagaaaaaca ggtcattaca cggtttatac agatctttga
     7921 taaataatat ggtgattggt gtagacacag gtttttccag aaagcttgaa ctgatgggaa
     7981 ttgatacaga gcagagctta aaggtgaaaa tttagtacta atgctcggat attcacatcc
     8041 gacagtattt tgcccaccgc cggaaattac aattgaagta ccgacaccaa actctgtttt
     8101 ggtaaaagga atcagtaaag aacttgtagg acttgtagca gcaaagatca gatcattcag
     8161 aaagccagag ccttacaaag gaaaaggaat aaagtttgag ggagaagtag taagaagaaa
     8221 agcaggaaag acagcagcaa aataattata aagagattta aacaacaatg aatcaaatag
     8281 aaaaaagcag aaagagaaaa gagaggataa gatttcagat caggaaaaag ctacgcggta
     8341 cttctgaagt tccgagatta gtagtttaca gaagtcttga tcatatttat gctcagctaa
     8401 tagatgatgt aaattcaaaa acaattttga cagtatcaac aaaatcaaag gatatattag
     8461 aaaagctgtc aggagtaaaa ggtaaggttg aaaaaagcaa gcttatagga aaattaacag
     8521 cagaaaaagc tgtatcaaac agtataagtt ctgttgtttt tgacagaaac ggatatttgt
     8581 accacggaag agtgaaagct cttgcggaag gagcacgaga aggtggttta aaattctgat
     8641 taaaattagt atattgcacg ttccttgctt actgatttca gttaaatttt acaactctcg
     8701 ggtcgtctaa tggcaggaca ccggcttttg gagccgctcg tggaggttcg actcctcccc
     8761 tgagaacaaa aaaattacaa agcggatttt aaagaaattt aaatttaatg aaaaaaaaga
     8821 aaaattataa agccggagag ttagaactta aagacaagat ggttcatatc ggcagggtag
     8881 ctaaagtggt aaaaggcggt agaaggttta gcttttcatc agtgtctgtg gtcggagacg
     8941 gaaacggaca tgtaggaatt ggattaggaa aagcgaatga ggtaacagat tctatcagga
     9001 aaagtgttga agatgccaag aagaatgtga ttgaagttcc gatcataaaa ggaacaatcc
     9061 cacatgagat aattggaaaa tacggtgcag caaaagtaat gctgaagccg gcttcacccg
     9121 gaaccggaat aattgccggc ggtggagtaa gagctgtact tgagtccgca ggagtacagg
     9181 atgtactgac aaaattacag ggttcatcaa atccgcataa cgttgtgaaa gcaacaatta
     9241 gagctttatt attattgaac gatgcaaaga aagtcgctga cagaagagga attacagtac
     9301 aggaattatt tgcaaactaa ttacaaataa gtataatgtt aaagataaca caaacaaaga
     9361 gtataatagg aaggcttaaa aaacagaagc tgacaattga agctttagga ttaggcagac
     9421 caaattattt taccttaaaa aaagacacac cgcagatcag aggaatgatc aacgtggtca
     9481 agcatctggt aaaagtagaa gaaaattata aacagtaatt ctgtttcaga aaacaaagtt
     9541 cttaatagtc ctttgtcctg atgattaggt caaaggaatt ttaattaaat tcgaaagtaa
     9601 aagaaaattt tcaatggaca ttttaagtaa tttaaaatac gctgaaggct caagaaagaa
     9661 aagaaaaaga gtcggaagag gtcagggatc aggacatggc ggcacatcaa caagaggtca
     9721 caagggtcag cgttccagat ccggagcaaa gttcagagcc tggtttgaag gtggtcaaat
     9781 gccacttcag agaagagttc caaagtttgg atttaaaaat atcaacagag ttgaatattc
     9841 tgtgatgaat ttagatgata ttcagaagct tattgacgat aaaaaaattc ttgagacaac
     9901 tatagataaa gaatatttat tgaaaaataa atttttaaaa ggaaagaata agcctttaaa
     9961 gatattgggt aacggtgaaa taacaactaa attagatatt actgctgatg ctttcagtaa
    10021 aactgcgatc gaaaagatag aaaaagcagg aggaaaagca gttaaattat gagtggattt
    10081 tttgaaagtt tcagaaatat atttaaaatt gaagagctca gaaacaggtt attatttacg
    10141 cttgcaatcc ttgtagtagt aaggatcgga gctcatataa cccttccggg aatagacgct
    10201 tcattactgg cagaagcttc aaaaaatcag gattctaata cattattcgg attatatgat
    10261 atgtttgtcg gtggtgcttt ccagaatgca gcaatttttg cactgggaat tatgccgtat
    10321 atcagtgcat ctattattat tcagcttctt ggagctgtat ttccttattt tcaaaaactt
    10381 cagaaagaag gagaagacgg aagaaagaag atcaatcagc ttaccagatt tggaacaata
    10441 cctgttgcag cattacaggc ttggggtgta agtgtacagc ttggatcaag aacagttgga
    10501 gatctcagcg ttatcagtcc ggaaatttca agtgcgttct ttactatatc aacagtaatt
    10561 ttattaacag gtggtacggt ttttatgatg tggcttggag agcagatcac tgaacgcggt
    10621 attggaaacg gaatttcgct tattatattt atcggaatta ttgataaatt tccgtactcg
    10681 gtatttgatg aatatcagat agtttcatcc ggtgcaagaa atattcttgt tgagataata
    10741 tttattatcg gattcttact gataattgcc ggtgtaattg cagttacagt agctacaaga
    10801 aaaattccag tacagtatgc taaaagagta gttggaagaa aagtatttgg tggagtcaca
    10861 cagtatattc ctatgaaagt gaattctgca ggtgttatgc cgcttatatt tgctcagtct
    10921 ataatgtttg ttccaagtac agtactgtca tttttcccga acagtgaatt tatgcaggga
    10981 gtggcaaaat attttgatta tacaagttgg ttctattcgt tagtctatgg gttattgatt
    11041 gtctttttta catattttta tacagcaatt gcatttaatc caaaagatgt atctgacaat
    11101 atgaaaaagc aaggcggatt tataccgggt gtcagaccag gtaagcctac ttcggattac
    11161 attgataata ttcttacaaa gatcacatta cccggtgcaa tatttttagc gatcattgct
    11221 atcattccga catttttgat gaagcttgga gtcacaacag gattgtcaca gttcttcggc
    11281 ggtacaagtt tgcttataat agttggtgtt gcattggata ctttacaaca gattgagtca
    11341 catttattga tgagacatta tgacggattt atgaaatcag gtaagttaaa atcaagaagg
    11401 taagtttctt aaatgtcatt gataaagact gaaaaggaaa tagatctgat aagagaaagc
    11461 agtaagattg tagcaggtgt ttttgaacat ctcaaagatc atataaagga aggaataacg
    11521 actgcggaaa ttgataattt agtagaagat tttattttat caaaagatgc tttaccggca
    11581 ttcaaaggat acagagttgg taaaagagtt tttcctgcaa gcagttgtat atcagtaaac
    11641 gatgaagtag ttcatggaat acccggtaac agaaaactcg aatcaggtga tgttgtatca
    11701 gtagatgtag gagttaagaa aaacggttat tttggagaca gtgcatatac atataaagtg
    11761 ggtgagactt cagcaaagat aaacaaactg ttgaaaataa cagaagagtc attatataaa
    11821 ggaattgaga tggcaataga cggaaatgaa gtaaatgata ttgcagtagc aatacaaaat
    11881 tatgtggaag gttcgggata cggagtagta agagagcttg taggtcatgg gatcggtaaa
    11941 aatcttcatg aagagccatc agttccgaat ttttattcac caggttcaaa acagagatta
    12001 tttgaaggta tggttttggc aattgagccg atggtaaatt atggtacata tggggtaaag
    12061 caggacaagg acggctggac aataagaaca caggacggag aaccgtcagc tcattttgag
    12121 catacagttc ttgttaaaaa aggaaaagca gaaattttaa caaagtgtta ataattgaaa
    12181 ttataaatgg ctaagcagga cgcaataaaa gtggacggaa ttatttcaga gatactgcca
    12241 aacaccaatt tcaaagtaaa attggaaaac ggccacgaga tacttgcgca tatttcagga
    12301 aagatgagaa tgaattatat aaggatatta cagggtgata aagtaacagt tgaattgtcg
    12361 ccatatgatc ttaccaaagg caggatcact tatagataca aatagataca aatagataca
    12421 aataaaataa aatttaatta actacatttt atagtcatga aagtacgaag ttcagtaaag
    12481 aaaatgtgtt caaagtgcaa agtcatacgc agaaaaggtg tagtgagagt aatttgcact
    12541 aacccgaaac acaaacaaag acagggataa tttttttaat aattataatt tttcaggagt
    12601 aatagtttgg caagaatagc aggagtagat cttccaaaga acaagagagt ggtaatagga
    12661 ttaacgtcta tatttggaat aggcaaagcc actgctcaga aaattctggc aaaaaccgga
    12721 attgatgaag acagaaaagt atcagcactt tctgatgatg aagtaaatca gatcagagca
    12781 gagattggta ctgagacgaa agtcgaaggt tcattaaagt cagagatcgc gatgaacata
    12841 aagagactga cggagatcgg atcatacaga ggcagaaggc acagaagagg attaccggtc
    12901 agaggccaga ggacgaagac aaatgccagg acgagaaaag gaaagagaaa aactgttgca
    12961 ggtaagaaaa aagcagcagc taagaagtaa gaattaatca ctgcatttaa cttttaaaaa
    13021 gatttataaa agtaatacaa aataaataga ctaaattggc aaagaatttt aaaaagacca
    13081 agaaaaaagc aaatgtagaa gcaaacggtg tagctcatat taaagctaca tttaacaatg
    13141 taattgttac attgaccgat ctttatggaa atactgtatc gtgggcttct tcaggaaaaa
    13201 tgggtttcaa aggctcaaag aaaaatacac cgtttgcagc acagcttaca gcagaaagtg
    13261 cagctaaggg tgcaatggat cttggaatga aaaaagtaga agtacttata aaaggagtcg
    13321 gatccggcag agatgctgcg gtcaggtcac ttaatactgc aggtctggaa gttacatcta
    13381 taaaggatat aacacctttg ccgcataacg gatgcagacc tcctaaaaag agaagaatgt
    13441 aataaattta gaaacagaat ttaatatttt aataaagaaa aataatataa atggcaagat
    13501 atacaggcgc taattgtaaa ctgtgtcgca gagaaaagca aaaattattc ctaaaaggaa
    13561 caaaatgttt ttcagataaa tgtcctatcg agagtagaaa ttatcctccc ggtcagcatg
    13621 gaaattcaag gcgaacaaga atgacagact atgcagtaca gctgcgtgaa aagcaaaaag
    13681 tcagaaagac ttacggagta cttgaaaatc aattcagaag atattttgag gctgcaaata
    13741 agaaaaccgg tgtaaccggt gataatcttg ttaaacttct tgaaagaaga cttgacaata
    13801 ctgtttacag attaggactt gctccttcaa gaaaatctgc cagacaatta atcctgcaca
    13861 gacattttac aataaacgga aaagttgtaa acataccgtc gtttcttttg aaatcaggtg
    13921 atttgataca ggtaagagaa aaaagtaaaa aacttgatat tattcatgat tcaatgaaaa
    13981 gagtaaaaga taatatgctg ccttcctggc ttaatcttga caaagcaaat ttacagggag
    14041 tattcatgaa agagcctgaa agatcagacg tacagtttat tggaaatgag cagctaattg
    14101 tagagctgta ttcaaaataa tttttttaac aaaataaata agagtatata atgaaaatta
    14161 ataattttca aatgccggaa tcaattatca aagaagagtc aacttatact gatactttcg
    14221 gaaggtttgt aatgcagcct cttgaaagag gatacggagt tacattgggt aattcactga
    14281 gaagaatatt gatctcttct ctgcccggtt cagcaattat agcaataaga gtaaacgatg
    14341 cgccgcatga atttactact ttaaaaggag tggtagaaga tctttctgaa ataattctga
    14401 atctaaaaga agtaagattt aaagatatta ctaataaatc ttcaaaaatc gaatttaact
    14461 taaaaggtca gcagatattt acagctcaga atattcagga tagtactgca gattttgaag
    14521 tgttaaatcc tgaaaagcat attgctacat tgaataaaga tgcaaatctg aacatagaaa
    14581 tcagaatggg atccggattc ggttatgtac cttcagatga taataagcag gcaaatcttc
    14641 cactggaatt tatttatgtg gattcaattt tttcacctgt tgtaaaggca aattatgtaa
    14701 tggaaaatac gagagttgga aagagaacag attatgaaaa acttacactt gagataacta
    14761 ctgacggttc gatcaatcct gaagatgcat tggtaaattc tgccagatta atgcaggagc
    14821 atattgacat gttcctgaat ctgaacccag aaaatgtgaa aaaaatggaa gagccggtca
    14881 gaacagaaaa agatgaagag catgaaatga tcaaaaaaat acttttaatg cctgtagatg
    14941 agttagatct ttcagtcaga tcacaaaatt gtttgagatc agcgaatatt aaaacgattt
    15001 cagatcttgt aagtaagaac gaaggtgaaa tgcttcattt cagaaacttt ggaagaaaat
    15061 cacttgcaga acttggagag ctgatcgaga atttcggttt gacatttgga atggatgttg
    15121 ataaatacct taaagaagaa acacctgtat aataaataat ttaaaataaa gataatttag
    15181 atatatatgg tacacagaag aaaaggcaga aagtttaaca ggacagccag tcacagaaga
    15241 gcgcttcttg caaatttatc cattgctctt attttaaata aaaggataaa gacaacagaa
    15301 gctaaagcaa aagagctcag aagatttat
//
LOCUS       DAORVQ010000066        15325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1701_length_15325_cov_5.021218, whole genome shotgun sequence.
ACCESSION   DAORVQ010000066 DAORVQ010000000
VERSION     DAORVQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15325
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1701_length_15325_cov_5.021218"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLX80_07090"
     CDS             complement(<1..422)
                     /locus_tag="PLX80_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20590.1"
                     /translation="MDDKKIEIIKREKEDYIDLSRSEAENHYKAEASKEVIKTERPKN
                     VAINKVTDFFESKFFSWIFHYIKSRFGLRHKFQSYELESDKGIYKMEKSNNDGKDEIT
                     IALAADWATDTAESDIIGDLIEDKDPDYTIHLGDTYFVG"
     gene            complement(452..4009)
                     /locus_tag="PLX80_07095"
     CDS             complement(452..4009)
                     /locus_tag="PLX80_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQY20591.1"
                     /translation="MPSIFNFSGYYKPDPLDKAPAAAQYIINIIVTSVITAVMVYCIT
                     DVFGLLEFESMGVKIMEVIVFGLIFAPMIVFFRKSSSLFLILIIFIPLFIFDIYLQAN
                     VRDANESALWIYLPGTFIDDVKILPLRFLMTLSFDGLIAGPLCIWISRLIAKLIYGKR
                     KLPGADYEDRVKLFNEEWSKETIEKPKRDFGFYVLRILGFAYIAYLFILIIGMLGHTP
                     WPEQIGNLISMTYVNPALAVNTFSKIGIMILFTFIGAYNRKARYYCCWGLIVGHAVST
                     VSSLGFYFYAFNAPQISEFLLTSAIVDGVMIVLFFIILFQCRSESFWIRDEKDFPKNF
                     SVPSQIMKISLYIVSAVSFGMVLLALYFRLFTDGSSGLGAVYGYPDPSLGNTLTLYFT
                     ISFLAFILAKSLVLRKYLYGILLFPFFIGSAAAVIIYFVMEYLNELTITTRGGSFIRP
                     DWYFMMFIMFSSLIFIFLTAVRKLYYEVDFMISSLNPSSAKNVIALSEAFFGKENGMD
                     NKNDNGMDKRKNSGILKAIDNYISGISGRKRGLLNFPFWLIENLFNLIFGLHPGLSVM
                     SREEQRWFLKKYMLRDPVECSKSFVPMFADMGFQIGLAANAMIMFANYSDINVRKKIG
                     YVPFYARDRMQKEIPTDGAPYENIFPLPENPEHENNFRKPTDEYDKKFVTPRILTSVE
                     EKEIPDEADYVIIGSGAGGAVMAYRLAQKVSDPSKILLIERGNRYQPLQDFSDNEMEM
                     MRKLYKEGGLQQTKKFTMSVLQGECLGGTTVVNNAICIEMDDEIKYKWQNEYDIDLSS
                     LSEEYERIAKEIDIQELDEKGINKNVSAKFKKGVKGFNENSDEKLKTLFPLKANHRNL
                     IGDENWNLGNKRQTKRTMLETYIPWSEARGVKVLSNLSALKFTHSGRKADSVIVRTED
                     GEIKKVKVNKAVIAAGGVIASSHFFMRSGIKVNVGKRMSCNFAFPVTFQFEDILNAFD
                     GNQITLGALDEKNRAVFETYFNPPASFALSSIPFYFGRRNDMMKNYTRSANFGALVGS
                     EPNGEIQTKADLLNGQPFEWELGNMDRENIRYAFNTILKLGYYAGAKKAILPMKPGIE
                     IDLSIQNLGKFEKLFNEYPLTISDLLIGTAHPQGGNIMTGDKSIHKSKRVLDQNYRLD
                     GFENVFAADASVFPESMRVNPQWTIMAMSAMASEHVLKI"
     gene            complement(4239..5198)
                     /locus_tag="PLX80_07100"
     CDS             complement(4239..5198)
                     /locus_tag="PLX80_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS cGAMP-activated phospholipase"
                     /protein_id="HQY20592.1"
                     /translation="MKKILTIDGGGIRGVLPARILHEIEKRTKKPISEIFDLIAGTST
                     GGILALCLTKPKENGKPRYKALDILKLYEDEGKKIFKKSPWKSTMSMGNLVDEKFSIA
                     GIEEVLEVYLGETKLSESLTNLIIPSYELEQRIPFFFKSRKAKESEDYDFKMRDVARA
                     TSAAPTYFESLKLRASPPVDYFALIDGGVFANNPSMCAYAEAIKEFGSDEEILLVSLG
                     TGELTRRISYEESKNWGLIGWARPLLDVVFDGVSRTVEYQLCQILNSDPNKKRFYRFQ
                     TKLDIGNDNMDDASATNIRVLKLKAEEMINSNKDEFESLCKLL"
     gene            complement(5229..5900)
                     /locus_tag="PLX80_07105"
     CDS             complement(5229..5900)
                     /locus_tag="PLX80_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HQY20593.1"
                     /translation="MVDKENFFKKYPYRPLKQMGVVTIENIIDHYDEDKGYRNLRELA
                     YVLATAYHESAHTWNPSITEYGKGKKRKYGMPHAKTGQIYYGRGLCQLTWHYNYESFT
                     KILGVDLVNEPDLALEPRNSVDILMIGMRDGIFTRHKLNMYFDKDTTDWVGARRIVNG
                     LDKTNLIASYAKAFYRGLDYKKEEEKSAEESAIVNIQNPKKSLAETEAAAVHRVELEG
                     RIVKP"
     assembly_gap    6745..6844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7186..9558)
                     /locus_tag="PLX80_07110"
     CDS             complement(7186..9558)
                     /locus_tag="PLX80_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20594.1"
                     /translation="MKLISKYKFFKIFTNSIFISKILFVTLLILIGKNISAKENTSVI
                     KGKVIDKETSSILIEVNISLENIDNGEKFNTRSEISGEYSFSKIKSGSYNLKASHIGY
                     SDYEEVVIINSSEVLKKNIYMEQAEIEIDKINVTSSKTEVTLQQTPSSISVVGSEEIN
                     NKNILTFDKVLEEVQGVTINRSSGINVNSLSIRASSDVAGGGIGNRVLLLLDGRPSLT
                     GDSKGALWSLIPVSIIERTEVVKGAFSSLYGSSAIGGVVNVITKKPTYKSYTAVNMNY
                     GFYEKLPDSLSFSNKLQSISGANIIHSNTIKKFSYLANLNYSYNDGYSQQSGYDFYSL
                     TGKVTYDLFSNRDLEATIQYTSSESDFPRYWKKDAGQTAEPFKVADFYIGDYINKKSQ
                     SYDLFYRAIPTSNSKYTTRFYYYYLNSNSYYNPNNPVSIQFADTGLGLNTFIDSYNIG
                     NITQMDFQLGRSNYLLTGLDVQINIVKSDPEEILYGDQQMNNFGLFAQDQWKMIQDIN
                     GNDILTSTIGLRADFNQVVSGINSFQLSPKLSFVYSPVSSNLVFSNTAFRLLGGRAFR
                     APSIAELYFKKELFGGFDFLYNPNLKPEEMLSFEVGMRKQYLNRFNIDMAFFYNYYTN
                     LIQYVNVGGGSLYGPFQVQNVANTQVKGFEFYIDYNSDFKLFSSPVNYYFNIGYTYLQ
                     ARDLSENRENDFLPYKPENNFNFTANMNYYDFNLNVNGRYLSEIGEVLFYDFEEPDSY
                     FLLNLKLSKQITSKLSFFISVDNLFNESYQELERIQAPNRNYNSGLFFEF"
     gene            complement(9567..10088)
                     /locus_tag="PLX80_07115"
     CDS             complement(9567..10088)
                     /locus_tag="PLX80_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20595.1"
                     /translation="MNKIFNNPLLSAVFVLLITITIYSCSDDENNVIVPVGPAANTVN
                     GVVTFVDTNFILTGGTYLISAYPSTGWPPMSGPTAFDTISIKRTNNVLDLQYSYTLKD
                     LNPGSYVISVGFRKATGGQSPIMSVYGCDTARAQYSPCFLSPPKNATIGTENQGTEGI
                     SMLSWSDTTNKVY"
     gene            complement(10372..10710)
                     /locus_tag="PLX80_07120"
     CDS             complement(10372..10710)
                     /locus_tag="PLX80_07120"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HQY20596.1"
                     /translation="MQKEEKKFVAINYIDCKDHYRERFEELFGSRAKAIDRMPGFHNM
                     EVLKPMDGGNIYLIVSHWDTEDAFKGWTKSPEFLEGHKRGFEDIKIAKEKGEELPMTS
                     DFKTYEVIAR"
     gene            complement(11074..12258)
                     /locus_tag="PLX80_07125"
     CDS             complement(11074..12258)
                     /locus_tag="PLX80_07125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQY20597.1"
                     /translation="MKIVLKKNEDRRLLSGHQWIFSNEIDSIIDFEKNGQITDLYSAS
                     GSFLGKGFYNKNSLIAYRHLTSKPEEEIDKNFIFKRISMANSLRKKKVNSGEVYRLVN
                     SESDFLPGLIIDKFGDKYSIQIFSAGMELFKKEITEVLDTNFKADLVVEKNDNEIRTL
                     EGLEKTEGILFDRSGSEAGKFEVEIDGIKINIDLLKGQKTGYYLDQQENRTLLRKYIK
                     EGDKVLDLFCNEGGFALNASLAGAEIITAVDSSEHSINMAEGNAMLNEIKNIDFKKAD
                     VFELVNEYFQKTDRFDFVILDPPSFTKSKKNIYTAIKGYTELNYKAMKLLKPNSFLLT
                     FSCSHHISEKAFEEMLVQSAEKAKRKIQIIEFRNSSYDHPVLPQMPETKYLKGYLLRV
                     TV"
     gene            complement(12280..13236)
                     /locus_tag="PLX80_07130"
     CDS             complement(12280..13236)
                     /locus_tag="PLX80_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20598.1"
                     /translation="MPEYKKILVVRLSSLGDIILSFPLLKILRKKYPQAGLHFLTRKK
                     FSEVITLNPNVDKIIETDSDLSITKNKIHDEKYDLILDIHKNFKSIYLTRGSGAEVRR
                     IVKNNFMKMILVKFKINLFKEIIPVYKKYLHTLSKNLTEDELKFTTGDLKISVEKKFD
                     FPYAVISPSARHFTKTYPAAKFIEFIRFRENETFILTGSKDDIDMEICSKINSECSNT
                     KNLCGELSISELASVIRGSEYVICNDSAALHLSEALNKKTYAIFGSTVKEFGFFPQIE
                     TSEVLEVNGLNCRPCSHIGLPKCPENHFKCMMDIDLKKFNIK"
     gene            complement(13252..14907)
                     /gene="bshC"
                     /locus_tag="PLX80_07135"
     CDS             complement(13252..14907)
                     /gene="bshC"
                     /locus_tag="PLX80_07135"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HQY20599.1"
                     /translation="MSIDYTSIPSFSELFIKYINDFSSISKFYEYDFRSDSDFRECIE
                     IKKESYANNKNFFRNELVEVLKEQNKIFGSGDETFENIQILNEKDSFAVVTGQQLGFL
                     TGPYYTILKTLNTIQLCEKLREKFPEYKFVPVFWLEADDHDFLEINNVNIISKENELK
                     NVSYYVGGEEKEKYYEPTGNIKLDGRISEYIDEVENSMNGTDFSKDLFDNIRKAYTEG
                     ADLKTAFAKLLNNLIGDKGLIFIDATDERLKKMLKPVFHKELSTFPAVCEKVIDTSVE
                     LEENFDVQVKPKAINLFYIHEGNRYLIEPRDGEIFALRHSRTKFSKEELFELLESNPE
                     RFSWNVVTRPICQDYLVPTIAYVGGPSEISYFGQFREVYKWFNIPMPVIYPRTSITIL
                     EKKVIVFLEKYGLKFEELFDEKELSKKLIGKTNEYNADEIFSEFKDELRALFYTYEKT
                     LENIDANQTKALSKRNEQFLESMNVAKDRFSDAQSKSSEVISNQLQKILINVYPDNTL
                     QERVLNITYFLNKYGPAFVDHLFLHTDISDFSHQLVDTNLSLK"
BASE COUNT         4728 a         2831 c         2235 g         5431 t
ORIGIN      
        1 cctacaaaat aagtatctcc tagatgaatg gtataatcag gatctttatc ttctatcagg
       61 tctccgataa tatcagactc agcagtatcc gtcgcccagt cagcagcgag tgcaattgta
      121 atttcatctt taccgtcatt atttgatttc tccattttgt aaattccttt gtcactctct
      181 aattcataag actggaattt atgccgaaga ccgaacctgc tttttatgta atggaatatc
      241 caactaaaaa attttgattc gaaaaaatca gtgactttat ttattgcaac attttttggt
      301 ctttcagttt ttattacttc cttgctggct tctgctttat aatgattttc tgcctcgctt
      361 ctggaaagat ctatgtaatc ttctttttct ctttttatta tctcaatttt tttgtcatcc
      421 atttataaga tctgtattta ttttcaggtt tttaaatttt aagtacgtgc tctgaagcca
      481 ttgcgctcat tgccattatc gtccactgag gattgactct catactttca ggaaagacag
      541 atgcatctgc agcaaataca ttttcaaatc catccagccg gtaattctga tcaagcactc
      601 ttttagattt gtgaattgat ttatctcctg tcataatatt cccgccctgc ggatgtgccg
      661 tacctataag cagatcgctg atggtgagtg gatattcatt aaaaagtttt tcaaactttc
      721 ctaaattctg tatagataga tcaatttcaa ttccgggttt cattggtaaa attgcttttt
      781 tagctcctgc ataatatcca agttttaaaa ttgtattaaa tgcataacgt atattttctc
      841 tgtccatatt accaagctcc cattcaaaag gctgcccgtt aagtagatcc gctttggtct
      901 gaatttcacc attcggttcc gagccgacaa gtgctccgaa atttgctgac ctggtataat
      961 ttttcatcat atcatttctt cttccaaaat aaaaaggaat cgaagacaat gcaaatgatg
     1021 caggcgggtt gaaatatgtt tcaaatactg cgcgattctt ttcatccagt gcaccaagtg
     1081 tgatctgatt tccatcgaat gcatttaaaa tatcttcaaa ctgaaatgtc acagggaatg
     1141 caaaattaca gctcattctt tttccgacat taacttttat accactgcgc ataaagaaat
     1201 gactgcttgc aattactccg cctgcagcga tcacagcttt atttactttt acttttttaa
     1261 tttctccgtc ttcagttctc actattactg aatccgcttt cctgcctgaa tgagtaaatt
     1321 ttaatgctga caaatttgat aaaaccttta cacctcttgc ttcactccat ggaatgtatg
     1381 tctcgagcat agttcttttt gtctgtctct tattccccag attccagttc tcatctccaa
     1441 tcagatttct gtgatttgct ttaagcggaa ataatgtctt tagtttttca tcagaatttt
     1501 cattaaaccc tttgacacct tttttaaatt ttgctgatac atttttatta attccttttt
     1561 catcaagctc ttgtatatca atttccttag cgatcctttc atattcttca cttaagctac
     1621 tgagatcaat gtcatattca ttctgccatt tatattttat ttcatcatcc atttcgatac
     1681 agattgcatt attaacgaca gtagtcccgc caagacattc tccctgaagt actgacattg
     1741 taaatttttt agtctgctga agtcctcctt ctttataaag ctttctcatc atttccattt
     1801 cattatcgct gaagtcctgc aatggctggt atctgttgcc gcgttcaata agaagtatct
     1861 ttgacggatc ggaaactttt tgggcgagcc ggtaagccat tacagctcca cctgctcctg
     1921 acccaatgat tacataatct gcttcgtcgg gaatttcctt ctcttctaca gaagtcaaaa
     1981 tccggggagt tacaaatttt ttatcatatt catcagttgg ctttcgaaaa ttattctcat
     2041 gttcaggatt ttccggaaga ggaaaaatat tttcataagg tgctccatca gtcgggatct
     2101 ctttctgcat tctgtctctg gcataaaatg gtacatagcc gatctttttt cttacattta
     2161 tatcagaata atttgcaaac ataatcattg cattggctgc aagtccgatt tgaaaaccca
     2221 tgtcggcaaa catcggcaca aatgattttg aacattcaac aggatctctc agcatatatt
     2281 ttttcagaaa ccatctctgc tcctccctgc tcatcaccga caaacccggg tgcagtccga
     2341 atataagatt aaataaattc tctatcagcc agaatggaaa attaagaagt cctcttttcc
     2401 ttccgcttat tccgctgata taattatcaa tagcttttaa aattccggag ttctttcttt
     2461 tgtccattcc attatcattt ttattatcca ttccattttc ctttccaaaa aaagcttcgg
     2521 aaagtgcaat aacattctta gcactggagg gattcaatga tgatatcatg aagtcaacct
     2581 cgtagtataa ttttcggacg gctgtaagaa aaataaatat cagagaactg aacattataa
     2641 acatcataaa ataccagtct ggccttataa aactgccgcc tcttgttgtg atagtaagtt
     2701 cattcaaata ttccattaca aaataaataa tgaccgcagc tgcagatcca ataaaaaatg
     2761 gaaataaaag aatgccgtat aaatattttc ttaatactaa actctttgct aatataaatg
     2821 cgagaaatga tattgtgaaa tataatgtga gtgtattgcc taatgaagga tccggatatc
     2881 cgtaaacagc tccgagtcca ctgctgccgt ctgtaaataa tctgaaatat aatgcaagta
     2941 aaaccattcc aaatgaaact gctgatacta tgtataaact tattttcatt atttgtgaag
     3001 ggactgaaaa atttttcgga aagtctttct catctctgat ccagaaactc tcggatctgc
     3061 actggaaaag aattataaaa aaaagtacga tcattacgcc atcgactatt gctgatgtca
     3121 aaaggaattc cgaaatctgc ggagcattaa atgcataaaa ataaaatccg agtgagctga
     3181 ctgtagagac tgcatgtccg actatcagtc cccagcagca ataatatctt gcctttcggt
     3241 tataagctcc gataaatgta aacaggatca tgatacctat tttcgaaaat gtatttactg
     3301 ccagtgcggg atttacatag gtcatcgaaa taagatttcc aatctgctca ggccagggag
     3361 tatgtcccag cattccgatg atcagtataa ataaatatgc aatatatgca aagccgagta
     3421 ttctcaatac ataaaatcca aaatcgcgct taggcttttc tatagtttcc tttgaccact
     3481 cttcattaaa aagttttact ctgtcttcat aatcagctcc gggtaatttt cttttgccgt
     3541 atattaattt cgcgatcaac ctgctgatcc agatacacag cggtcctgcg atcagaccgt
     3601 caaatgaaag tgtcatcaga aatcttaatg gcaagatctt tacatcatct atgaaagtcc
     3661 cgggaagata gatccaaagt gctgattcat ttgcatccct cacatttgcc tgcagataaa
     3721 tgtcaaatat aaatagtggt ataaaaataa ttaatatcag gaaaagagaa ctcgactttc
     3781 ggaaaaagac tatcattggc gcaaatatca aaccgaatac aatcacttcc ataattttaa
     3841 cgcccatact ttcaaattcc agcagaccga aaacatcggt tatgcagtaa accattactg
     3901 cagttatcac ggaagtaaca attatattaa ttatatattg tgcagccgca ggagctttgt
     3961 caagcggatc aggtttgtaa tatccactga agttaaaaat agatggcatg atcataattt
     4021 aaattaatga ataactgaag ttacgcaatt taccgatatt tactgcaaac agaaagatgc
     4081 tagtttccaa actctcagac tttccaaaac gcctctaagg ctctaagact caaagtttaa
     4141 gaactttaaa aacaacattc ttagagcctt cgagtcttag aggtaacctt aattgttccc
     4201 gcagagtcaa gtgcttttcg gactctgcgg aaactgaatt acagcagttt acaaagcgat
     4261 tcaaattcat ctttattact gttgatcatt tcttcagctt tcagttttag tacccggata
     4321 ttagttgctg aagcatcgtc catattatca tttccgatat caagttttgt ttgaaatctg
     4381 tagaatcttt ttttattcgg atctgaattc agtatctggc aaagctgata ttcgactgtc
     4441 ctgcttactc cgtcaaatac tacatccagt aaaggccttg cccagccgat cagtccccag
     4501 ttctttgatt cttcataaga aattctccta gtgagctctc cagtaccaag tgataccaaa
     4561 aggatttcct catcactgcc aaactctttt attgcttcag cataagcgca cattgacgga
     4621 ttgtttgcaa atactccgcc gtctatcagt gcaaaataat ctaccggggg cgaagctctt
     4681 aatttaagac tttcaaaata agtcggcgct gcgcttgttg ctctggctac atctctcatt
     4741 ttaaaatcat aatcttcaga ttcttttgct tttcgggatt taaagaaaaa tgggattctt
     4801 tgctcgagtt cataacttgg gataataaga ttcgtgagtg attccgaaag ttttgtttca
     4861 ccgagatata cttctaatac ttcttcaatg ccagctattg aaaatttttc atcaaccaga
     4921 tttcccattg acatcgtgga tttccatgga gattttttaa agatcttctt accttcatct
     4981 tcatacaatt tcagtatgtc aagagcttta tatcttggct taccgttttc ttttggcttt
     5041 gtaaggcaaa gtgcaagtat tccacccgtc gatgtccccg caatgagatc gaatatttcc
     5101 gaaattggtt tcttcgtacg tttttcgatc tcatgcagta ttcttgcagg aagtacacct
     5161 ctgatcccgc ctccgtcaat ggtcagtatt tttttcataa taatttttat attcagatta
     5221 aattttaatc agggctttac aattcttccc tcgagttcta ctctatgtac agccgctgct
     5281 tctgtctcag ctaaagattt tttcggattc tgaatattaa cgatcgccga ttcttctgca
     5341 gatttttctt cttctttttt ataatcaagt cctctgtaaa aagcttttgc ataagatgca
     5401 atcagattcg ttttgtccag tccgtttaca attctgcgcg caccaaccca gtcagtagtg
     5461 tctttgtcaa aatacatatt cagtttatgt cttgtgaaaa ttccgtcacg cattccgatc
     5521 atcagaatat ccacactgtt cctgggttca agagcaaggt caggttcatt aacaagatca
     5581 actcctaaga tctttgtaaa actttcataa ttgtaatgcc atgtaagctg acataatcct
     5641 cttccataat agatctgacc ggtctttgca tgcggcattc cgtactttct tttctttcct
     5701 tttccgtatt ccgttattga aggattccat gtatgcgctg attcgtgata agctgtcgcc
     5761 agtacatatg ccagttctct cagatttcta taacccttgt cttcatcata gtgatctata
     5821 atattttcaa tagtaactac tcccatttgc tttaatggtc tgtatggata tttcttaaaa
     5881 aaattttctt tgtctaccat gatatgctgt ttttaattaa taaaaaatta ttcagtgttt
     5941 ttaattttta aaaatttcaa aaattggtta agtattaaat tataaacaaa aagtatattc
     6001 actttgaaac ttcaaaaaaa tttcagcttt atatctattg ctttaatatg aaaatttata
     6061 cttaatgaat ttattaatga aaaatcatta cagtctttta tatgaaattt ttcggaaggc
     6121 cagaatttat ctgaaatggt agtaatgaat ttggtattaa tattcatcag ggagtaagtt
     6181 taatttaata tataataatt tttttttgag gaaaaataaa taaccattta tatcttttat
     6241 attttcaaaa attttctaaa aaagaaatgc agctccggat tccagaccgg agcttttatt
     6301 gaattgtaat aatgcgggta agtcctgaaa tgaaattacg aagtgtcata gatttgtaat
     6361 gtttaatttt aacaaccgat acaaatataa tgacaactca cagtaataat aataaaggca
     6421 cttaccgaaa atttgtaagt gttctcactt aactttgaat gagtaaaata ctgaatattt
     6481 taattgtgat aatttaaaaa ggaacaagcc ctgtatcaaa ttacagggct tgcaaaaaaa
     6541 ggaattactt aaatcggcaa atatttgcag ttttgtagca agttactttg aagaattctt
     6601 tcaatattga tttattaatt taacaatatt gtagtttaca tgaattaagt atatatacac
     6661 aattcgtctg agtagacata ctcaatttca ataagtaaac atacttaaat gttgatttaa
     6721 agcaaaaaaa cgatgttttt aattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnaataag taaacatact taaatgttga tttaaagcaa aaaaacgatg tttttaattt
     6901 attaataatt tagggtgcca agaggtgttg aagactcaaa gtatttattt tttttaaaac
     6961 ttttcgactt tacgactttg agactttgag actttgagac tttgagactt tgaggctttg
     7021 aggctttgag gctttgaggc tttgaggctt tgagactttg aggctttgag gttttgaggc
     7081 tttgagactt taaggttttg agactttgag actttgagac tttgaggctt tgaggctttg
     7141 tggctttaag gcaactgaaa aatgaaagaa aagaaataaa tttttttaaa actcgaagaa
     7201 taaccctgaa ttataatttc tgtttggagc ttggatccgt tccagctcct gataagattc
     7261 gttgaaaaga ttgtcaactg aaataaaaaa tgaaagctta cttgttatct gtttgctcaa
     7321 ttttaaattc aacagaaaat aagagtccgg ctcttcaaaa tcataaaata aaacttctcc
     7381 aatttcactg agatatctgc cgttaacatt cagattgaaa tcataataat tcatgttcgc
     7441 agtaaagttg aaattatttt caggtttgta tggaaggaaa tcattttccc tgttctcaga
     7501 gagatctctt gcctgcagat aagtgtatcc tatattaaaa taataattaa ccggtgaact
     7561 gaataattta aaatcagaat tataatctat ataaaattca aaacctttga cctgcgtatt
     7621 ggcaacattc tgtacctgaa aaggaccgta aagagaacct ccgcctacat ttacatactg
     7681 gatcaggtta gtataataat tatagaaaaa agccatgtca atattaaatc tgttcagata
     7741 ctgctttctc atccccactt caaatgaaag catctcctca ggtttcagat ttggattata
     7801 cagaaaatca aatccaccaa ataattcttt tttaaaatat aactctgcta tcgatggagc
     7861 tctgaaagct ctgccgccaa gtaaccgaaa tgctgtattt gaaaatacta aatttgatga
     7921 aaccggactg tacacaaatg aaagttttgg actaagttga aaagaattta ttcctgatac
     7981 aacctgatta aaatctgccc ttaatcctat agtagaagtc aaaatgtcat ttccatttat
     8041 atcttgaatc atcttccatt gatcctgagc gaataatcca aaattattca tttgctgatc
     8101 tccgtacaga atttcttccg gatcagattt tacaatattg atctgaacat caagtcctgt
     8161 caaaagataa tttgacctgc ccagctgaaa atccatctga gtaatattcc cgatattata
     8221 agaatcaata aatgtattta gacctaaacc tgtatcagca aactgtatgg aaacgggatt
     8281 gtttggattg tagtaagaat tgctgtttaa ataataataa taaaatcttg tagtatattt
     8341 tgaattactg gttggtattg ctctgtaaaa aagatcataa ctctgagact ttttattaat
     8401 gtaatctccg atataaaaat cggctacttt aaatggctcg gctgtctgtc cggcatcctt
     8461 tttccagtat cttggaaaat cactttccga actcgtgtac tgaatcgtag cttcaagatc
     8521 cctgttactg aaaagatcat aagtaacttt tcctgtcaga ctgtaaaaat catagcctga
     8581 ctgctgtgaa tatccgtcgt tataactgta atttaaattt gccagatatg aaaacttttt
     8641 tatagtgttg ctgtgaatta tatttgctcc tgatattgac tgtaatttat ttgaaaaact
     8701 caaactgtca ggaagctttt cataaaaacc ataattcata ttcactgcag tatatgattt
     8761 ataagtcggc tttttagtta tcacatttac tactccgcca atcgcactcg atccgtacag
     8821 tgaagaaaat gcgcctttca caacttctgt tctttcaatt atagatacag gaataagtga
     8881 ccataaagct cctttggaat cacctgtcag cgaaggtcta ccgtcaagta aaagcaatac
     8941 tctgttacca ataccaccac cggctacatc tgaagatgca cggattgaaa gtgaattgac
     9001 atttattccg ctgctcctgt taattgtaac tccctgcact tcttcaagga ctttatcaaa
     9061 agtcagaata tttttattat taatttcctc cgaacctacc acggaaattg atgatggcgt
     9121 ttgctgcaaa gtaacttctg tcttggatga agttacattg atcttatcaa tctcaatctc
     9181 tgcctgctcc atgtaaatat tttttttaag tacttcactt gaatttatta ttactacttc
     9241 ttcataatcc gaatatccta tatgagaagc tttcagattg taagagccac tttttatttt
     9301 actgaaactg tattcgccgg atatttcaga acgtgtatta aatttttctc cattatcaat
     9361 attttccaga cttatattga cttcgattaa gatcgaactt gtttccttat ctatcacctt
     9421 ccctttgatt actgatgtgt tttcttttgc tgaaatattt ttcccaatta agattaataa
     9481 agtaacgaac agtattttag aaataaaaat actgttcgta aaaattttaa aaaatttgta
     9541 tttggaaatc aatttcattt taaaaattag taaactttgt tagttgtatc cgaccatgaa
     9601 agcatgctta ttccttctgt accctgattt tcagttccga tagttgcatt ttttggagga
     9661 cttaaaaaac aaggagagta ctgagctctt gctgtatcac atccataaac agacattatt
     9721 ggactttgtc ctccggttgc ttttctgaaa ccgactgaaa tcacatagct acccggatta
     9781 agatctttca gagtatatga atactgaaga tccaatacat tgttagttct ttttattgaa
     9841 attgtatcaa aagcggtagg accggacatt ggaggccagc ctgttgacgg atatgcagaa
     9901 atcagatatg tgccgccggt taaaataaaa tttgtatcta caaatgtaac tactccatta
     9961 actgtattag ctgccggtcc gacaggaact attacattgt tttcatcatc agagcaactg
    10021 taaattgtaa ttgttattaa taaaacaaat actgctgata ataacgggtt gttaaatatt
    10081 ttattcaatt tagttttaat ttttaatttt taatttataa tttatttcct gaatcaaatt
    10141 taataatgta tttctttaac aaatatgatt ttaatcataa ttgataaatt gttttattga
    10201 tatttataac gtaataattc aataggtaaa tatgatttta ttacaaataa atctgtttta
    10261 aaacagcttt aaaataatac aaggtataag ataccacatt agtcctttta caagaaaaaa
    10321 taataatcac cagaaaccga gtttccttac agctgatttt acttttaaga tttaccttgc
    10381 aatgacttcg taagttttaa agtccgaggt catgggaagt tcttcacctt tttcttttgc
    10441 aatttttatg tcttcaaatc cccttttatg tccttccaga aattccggag attttgtcca
    10501 tcctttaaaa gcatcttcag tatcccagtg actcacgatt aaataaatat taccgccgtc
    10561 cataggtttt aatacttcca tattatgaaa ccccggcatt ctgtctattg cttttgctct
    10621 ggaaccaaac agttcctcaa atctctctct atagtgatcc ttgcagtcaa tataatttat
    10681 tgctacaaat ttcttttctt ctttttgcat tattattttt tatttgataa aaatttcatc
    10741 tgcaaatata atgcttatta agactaagtt caaataagaa aatttactct tttcaaaatt
    10801 cagagaatat tgctcgtaat aaaactcccg gactttctga aaatgtttga attgccgcta
    10861 tggctctaag acaagaagtt aaaaatcatt ctaaaaatat ttcttaaaat ctttgagaat
    10921 tggacacaaa ttttacaaat ttagaattgg acaaactttg gacagcttgt gcaaaataga
    10981 tttaattgcc ttaaagactc ccggacacaa attcaaatgt cgaaaaattt atttacttat
    11041 agccttcggg acctggaggg aaattaagaa attttatact gtaaccctaa gcagatatcc
    11101 cttcagatac ttagtttcag gcatttgcgg taagaccgga tggtcataag aagaatttct
    11161 gaattcaatg atctgtatct ttctttttgc tttttcagca ctttgaacaa gcatttcttc
    11221 aaatgctttt tcactaatat gatgggaaca tgaaaatgtt aataaaaatg aattaggctt
    11281 tagcaatttc atcgccttat aatttaattc tgtataacct tttatagctg tatatatatt
    11341 ttttttagac tttgtaaatg aaggcggatc taatatcaca aaatcaaatc tgtctgtctt
    11401 ttgaaaatat tcattcacca gttcaaatac atctgctttt ttaaaatcaa tatttttaat
    11461 ttcattaagc attgcattac cttcagccat atttatggaa tgttcagaag aatccactgc
    11521 agtaataatt tcagcacccg ctaaagaagc attcagcgca aagcctcctt cattacaaaa
    11581 tagatcaagt actttatcac cttcctttat atattttctc agtaaagttc tgttttcctg
    11641 ctgatctaaa taatagcctg tcttctgacc tttcaaaaga tctatattaa tttttattcc
    11701 atcaatttca acttcaaatt ttccagcttc acttcccgat ctgtcaaata aaatgccttc
    11761 agttttttcc aatccttcaa gcgtccttat ctcattatca tttttctcaa cgactaaatc
    11821 agctttgaaa tttgtatcaa gtacttctgt aatttctttc ttaaacaatt ccatccctgc
    11881 agaaaatatc tgtattgaat acttatctcc gaatttatct atgataagcc ccggcaaaaa
    11941 atcactctcg ctgtttacca gtctgtaaac ctcacccgaa tttactttct tttttcgaag
    12001 cgaatttgcc atactgatgc gcttaaatat aaaatttttg tcaatctctt cttccggctt
    12061 tgaagtcaaa tgtctgtaag caataagaga atttttatta taaaatccct ttcctaaaaa
    12121 acttccggat gctgaataaa gatctgtgat ctgaccattc ttttcaaaat caattatact
    12181 gtcaatctca ttactgaata tccactgatg accactgagc agccttctgt cttcattttt
    12241 ctttaatact attttcatct ttgtctgtaa tttataattt tatttaatat taaatttttt
    12301 cagatcaata tccatcatac atttaaaatg attctccgga catttgggaa gaccgatatg
    12361 tgaacatggt ctgcagttta aaccgtttac ttctaatact tctgatgttt caatctgagg
    12421 aaaaaatcca aactccttta ctgtcgatcc gaatattgca tatgtctttt tatttaacgc
    12481 ctcgctcaga tgcagggctg cgctgtcatt gcaaatcaca tactcacttc ccctgatcac
    12541 tgatgctaat tcacttatcg ataattcccc gcaaagattt ttagtattgc tgcattctga
    12601 atttatttta ctgcagatct ccatatcgat atcatctttg ctgcctgtta atataaaggt
    12661 ttcattttct ctgaaacgaa taaactcaat gaacttcgca gccggatatg tctttgtgaa
    12721 atgcctggcc gaaggtgaaa ttacagcata aggaaaatca aacttttttt ccacagatat
    12781 tttcagatcg ccggttgtaa actttaactc atcttcagtt aaatttttac ttaatgtatg
    12841 aagatatttt ttatagacag gaataatttc tttaaataaa tttatcttaa attttactag
    12901 aatcattttc ataaaattat ttttgacaat ccttctcact tctgcaccgc tgcctcttgt
    12961 gagataaatg cttttgaaat ttttatgaat gtcgaggatc agatcatatt tctcatcatg
    13021 aattttattt tttgtaatgc tgagatcgct gtctgtttca attatcttat caacattcgg
    13081 attaagcgta atcacttcag aaaacttctt tcgtgtgaga aaatgtaacc cggcttgcgg
    13141 atatttttta cgcagaattt taagcaacgg aaatgagagt ataatatcac caagagatga
    13201 aagtctgaca acaagtattt ttttatattc aggcaaatat atttgagaat gttatttcaa
    13261 acttaaattt gtatccacaa gctgatgtga aaaatctgaa atatcagtat gtaaaaataa
    13321 atggtcgacg aaagccgggc cgtatttatt caaaaaatag gtgatgttta aaaccctctc
    13381 ctgcagcgta ttatcaggat atacatttat cagaattttt tgaagctgat ttgaaattac
    13441 ttcactactc tttgactgtg catccgaaaa tctatccttt gctacattca ttgattcgag
    13501 gaactgctca ttccttttcg aaagtgcctt cgtctgattt gcatcaatat tctcaagtgt
    13561 cttttcataa gtataaaata atgcccgcag ttcatctttg aattctgaaa aaatttcatc
    13621 tgcattatat tcatttgtct tccctataag ttttttactt aactcttttt catcaaaaag
    13681 ctcttcgaat ttcaatccgt acttttccag aaatactatt actttttttt ctaatattgt
    13741 aatcgatgtc cttggataaa ttacaggcat tggaatatta aaccatttat atacttccct
    13801 gaactgcccg aaatatgaga tctccgaagg tccgcctaca tacgcaattg tcggcacaag
    13861 ataatcctga catatcggtc ttgttactac attccagcta aatctttcgg gattactttc
    13921 caataattca aataattctt cttttgaaaa ttttgtacgt gaatgtctga gcgcaaatat
    13981 ttctccatct ctcggctcga tcagatatct gtttccttca tgaatataaa aaaggtttat
    14041 agcttttggc tttacctgaa catcaaaatt ttcttcaagt tcgactgatg tatctattac
    14101 tttttcacat actgcaggaa aggtactaag ctccttgtga aataccggct tcagcatttt
    14161 ctttagtctt tcatctgtag catcaataaa aattaatcct ttatctccaa tcagattatt
    14221 cagtaatttt gcaaatgctg ttttaagatc agcaccttca gtatatgctt ttctgatatt
    14281 atcaaataaa tctttactga aatctgtacc gttcattgaa ttttcgactt catcaatata
    14341 ctcactgatc cgtccgtcaa gttttatatt tcctgtcggc tcataatatt tttccttctc
    14401 ttctcccccg acataataac ttacattttt taattcattt tctttgctga tgatattcac
    14461 attattgatc tcaaggaaat catgatcatc tgcttccagc cagaatacag gcacaaattt
    14521 atattctgga aatttctcac gtaatttttc acaaagctgt atcgtattga gtgttttaag
    14581 aattgtatag taaggacctg ttaaaaaacc aagctgctgt cctgtcacaa ctgcaaatga
    14641 atccttttca ttaagtatct gaatattctc aaatgtctca tcacccgatc caaagatttt
    14701 attctgctct tttaaaactt cgaccagctc attccgaaag aaatttttat tattagcata
    14761 tgattctttt ttgatctcta tacattctct gaaatctgaa tcacttctga aatcatattc
    14821 atagaatttt gatatgctgc tgaaatcgtt tatgtatttt atgaaaagtt cgctgaatga
    14881 cgggatagaa gtgtagtcta tagacattta ctaaatttat ttatttaaga aaaattaatc
    14941 attaatattc taatatgaaa tttattaatt ttaacgaaac ttaatttaaa taaaatttaa
    15001 gatcctgctc gttcccaaac tccagttttg gaacgcactt gaaattgata ctctgtttca
    15061 atttcaatgt attcagccgg ggatcgttaa aaaaagatca gtaaaagttt taaatattta
    15121 attttatatt aagacacttt tgaaaaaagt tttaaatcaa atttaaaata ttttatttta
    15181 ataactttta taaattacat ttatgaattt taagtaccta ctatttacaa cacctcccaa
    15241 acctccccct atctaagaga agggcaagtg tgggtattta gaaggaatat ttacatacaa
    15301 agatttatgt gaatcgagcc gttgt
//
LOCUS       DAORVQ010000067        15322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1702_length_15322_cov_6.584463, whole genome shotgun sequence.
ACCESSION   DAORVQ010000067 DAORVQ010000000
VERSION     DAORVQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_15322_cov_6.584463"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1258)
                     /locus_tag="PLX80_07140"
     CDS             complement(38..1258)
                     /locus_tag="PLX80_07140"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQY20600.1"
                     /translation="MSKKGYNILYKLLISVAAVSVVIYLNRGNFSGSSTPELNSGLTQ
                     AGMNLSGTDIKYDNSEINKSDKTNKNNMADSTIDNDPVKLHFDALLADTHNDLIWQVY
                     ERGADFNVNNTSTQSDIFKFREGGLDIQVFAVWIPMGKVKSSYSFVMSQIDRLQNFQN
                     ESNGGFEFAKTYEDIIRITNAGNICGLIGIEGGTAVESDPENVQRFFDAGVRYIGLTW
                     NNSNLIATSAKDAVTRGKSGGLTEFGKEVITKMNETGMMVDVSHLSEAAFWDVIEIST
                     APVIASHSNCYSLNPHYRNLTDEQIKAIAESKGYIGINFYDEFLVQGGKKYGAGIEDI
                     IAHMDHIKNLVGVDYIGIGSDFDGGISAPGDLRDATQYPELTKRLFEKGYTPDEIRKI
                     LGLNFLRVFKQVCG"
     gene            complement(1278..1544)
                     /locus_tag="PLX80_07145"
     CDS             complement(1278..1544)
                     /locus_tag="PLX80_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20601.1"
                     /translation="MEDFIEVHVTYDPMEANLIKAKLTDEEIPFKVTGDFNIAISMET
                     FNTELSRMALKRPIKFFVPEKDFEMAKVAINTDKSSFFEDDLEY"
     gene            complement(1548..2486)
                     /locus_tag="PLX80_07150"
     CDS             complement(1548..2486)
                     /locus_tag="PLX80_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQY20602.1"
                     /translation="MISFLIFLIVLIVLITVLKGQFKENLQVEKILNTLRVAVGVGIL
                     LAILFASIIQIGPGEAGVQILFGNVQDKVLRSGLNFINPLIEVQKLDIKTQAYTMSGV
                     NDQDDSEIRVKKSDPIQTLSSDGLTLILDVTVWFRLSADDTPELIRNIGVDYEAKIIQ
                     PAIRTAIRDIAVNFVATDIYSSKRDDYVNDVARKLENSFEGRGIILEKVLLRNVELPQ
                     KVREAIDEKISAEQRAQQMVYVLQKERQEAERKTVEATGVAEAQRIINSTISNSYLQW
                     KYIETLKELVNSPNNSFVIAPYDQKLMPMLNFNQGK"
     gene            complement(2510..3631)
                     /locus_tag="PLX80_07155"
     CDS             complement(2510..3631)
                     /locus_tag="PLX80_07155"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQY20603.1"
                     /translation="MKIGILGGGQLARMIIEECNKYAFEFHVLSNEKNSPAGQITKFE
                     TEGDWNDEKTLIEFAKNCDIITLENEFIDHKKLKAIEDTGKKVFPCSENIKLIQDKFI
                     QKKTLQANDIPVAEFTEVKDLSDVKKFAEEFGYPLILKSRTMGYDGKGNFTVNSEDDI
                     SKGINELKERGELMCERFVNFEKEIATQVVRNTSGEIKVYPVVETIQKDHICELVISS
                     GQFEGQIESKVNDIAKKIVTELNYTGVMGIEMFLNGNEFLVNELAPRVHNSGHYTIEG
                     TYTSQFENHIRAVMDLPLGNTNRSSANSIMINLLGKREGESRLKRAGVVLANENTYLH
                     MYGKKQTRHGRKMGHITVLNNEIQKGIDTAYTAAAELDF"
     gene            complement(3670..5337)
                     /locus_tag="PLX80_07160"
     CDS             complement(3670..5337)
                     /locus_tag="PLX80_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY20604.1"
                     /translation="MNNIKRNLIKIYPAILLAGIFLFVGFNLKNDDDIYKKINRNMEL
                     FGQVYREIALNYVDEIDADKFMTAGIEGMLATLDPYTNYVDEDSRDQLDLITTGKYGG
                     IGITVNIRDSMVVISEVMNGYEAQKKGLRVGDIFLEIDGVRLEKDEVKNMKSFVRGPI
                     GSEVNIKIKRDDDIIDFTLLRQEIILKNVSYFGYLGPESDGIGYIKLDRFTNISESEV
                     ENAIKTLKSERELKGLVIDLRDNLGGLLDAAIGILNKIVEKNSLLVITKGKREDSEKK
                     YFSKYEPMISAEIPVAVIINSKSASASEIVAGGIQDLDRGVIVGNKSFGKGLVQQIRD
                     LDKNAQMKITTSRYYTPSGRWIQQKNYFKENKYGVFIDDAKYNQTDFKTLGGRTVTAY
                     GGISPDVEVTVEPESEIHFALMAKDMYFKFADDYLKKNPGLTTIYPDEAFFLQFRDFL
                     TEQEFSYTSGIEKKIKEIEELASEKKYGTEMTDLLDKVKSEAEKEEETELELAKNEII
                     LKITDEINKRIITEEEQIKAALTYDKQVIEAMKILEDPVLYRSLLVK"
     gene            complement(5356..5970)
                     /gene="tmk"
                     /locus_tag="PLX80_07165"
     CDS             complement(5356..5970)
                     /gene="tmk"
                     /locus_tag="PLX80_07165"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQY20605.1"
                     /translation="MFITFEGIDLSGKSTQIKLLEEFLISKKKKVISVREPGGTGISE
                     KIRDILLDKKNLNMEYVTEFLLFSASRYQLTQEVIIPKLKKNYYVLCDRYFDSSTAYQ
                     GFGGKIDLKTIYSVNKIASGNLNPGITFLINISAEEFVKRKKAINKTEDRIEQKNLSY
                     YKKVIDGYLKISGDNKKRFVVIDGKKSIPEIHNIVKDHVLKFKK"
     gene            complement(6521..7291)
                     /locus_tag="PLX80_07170"
     CDS             complement(6521..7291)
                     /locus_tag="PLX80_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HQY20606.1"
                     /translation="MQNNFAKLLAEKTKDNPAFIHLDFIGLIRLGKTNEIRIDKFCEM
                     LKEIENEGGNICIPAFTLSYTRNEIFNMPESPAVNIGMLPEVIRQKFPDKRTSDGLFS
                     FTAIGNNFSKDNFIPKDTASFGDSLMAEVFEKDGYLASIGGVFRNSTEIHYLEKLLEV
                     DYRSDKIFEGDLIDTENNLHRQKVTYFCKNFDYNLWYDFSELEKELKNEGLMEIIKSD
                     DNSIYIEMIKFKTVFEFLKDKIKKDKNYLIRELKDKRT"
     gene            complement(7281..8657)
                     /locus_tag="PLX80_07175"
     CDS             complement(7281..8657)
                     /locus_tag="PLX80_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HQY20607.1"
                     /translation="MFLDFLTEEFKNNSQKTAIVWNDQPYSYQTLLELFEKRKAEISQ
                     ISDIKGKVVALKSDFSLESTSMFLALAENECIIVPVNSKTKEDVKRIYDISMVELLIE
                     IDNNNITFTETGKSSDHEYYNVLRERKHPGIVLFSSGTSGEPKAAVNDFTALLEKFKT
                     RRYALTTLNFLLFDHIGGVNTMLHTLSNGGKLVFTKERTPEFICRQIEKHKVELLPVS
                     PTFLNLLLISEAYKDFDLSSLKKITYGTEPMPEGTLKRLNIIFPDIKFQQTYGLMEVG
                     ILRTASQSNNSLWVKLGGEGYELRIVDDILHIKTPSGILGYLNYPTPYSDDGWFITGD
                     HVETQGEFFRILGRRSELINIGGEKVYPVEIETVLKEIDNVGEAIIYSEPNSITGNII
                     CAEVTMIRDEDKGEFRSKIKKYCRENLQSFKVPAKIIIADKIEYTDRFKKKQIKRILP
                     NSNSTDAK"
     gene            complement(8657..9355)
                     /locus_tag="PLX80_07180"
     CDS             complement(8657..9355)
                     /locus_tag="PLX80_07180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY20608.1"
                     /translation="MKNKVILITGTSKGIGKYLAEYYLNKNCIVCGCSRNKSDIKNKN
                     YRHFQLDVADETAVSETLRQIRKEFNRLDVLINNAGIASMNHFLLTPLKTIIDIYKTN
                     VTGSFLFSRESVKLMKKNKTGRIINMSTVAVRLNIEGESVYASSKAALNSFTKILAKE
                     ISPYNITVNAVGLAPMKTDLIKNVPDEKIEKLLNQMTVKRLMEFEDITNMTDFLINDN
                     SSFITGQVIYMGGV"
     gene            complement(9399..9710)
                     /locus_tag="PLX80_07185"
     CDS             complement(9399..9710)
                     /locus_tag="PLX80_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20609.1"
                     /translation="MTEQNKINELIFEVIKEINESLPSDKKIKVAADTVLYGSKSKLD
                     SFGLVNLIVGVEEKIQDETGISITIADEKALSRKNSPFLTVKTLSDYVSELLNNVQKE
                     E"
     gene            9898..10569
                     /locus_tag="PLX80_07190"
     CDS             9898..10569
                     /locus_tag="PLX80_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20610.1"
                     /translation="MDHNKISSIALFEKRWPVALTIIIVTFLLWMIPDRVSIVPNWTL
                     YVICVFMLIPIAAIGISKANPLWIKSEKYVLLFFLIIIGIGNLLNLSNLVQDMLYKSG
                     EISGLQLLTSSISVWINNILMFSLLYWQIDRGGPEARLNSSYKKADWYFSEESAPETF
                     TPKNWNPVFIDYLFLAFSTSTAFSATDSLPLTPKAKLLMMLQSIVSLTIIVVVGARAI
                     NILGS"
     gene            10980..13355
                     /locus_tag="PLX80_07195"
     CDS             10980..13355
                     /locus_tag="PLX80_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY20611.1"
                     /translation="MSTKNKKHRSHLPIPDQAYRGFIAYDGKDPDSKFPPIQTLLPPE
                     GAPNVLLILIDDAGFGSASAFGGPCNTPNAEKLAAGGLKLNRFHTTALCSPTRQAILT
                     GRNHHSAAMGSITETATGAPGYTSVRPNTMAPLAKTLKLNGYSTAQFGKCHEVPVWET
                     SPVGPFDSWPSGGGGFEYFYGFIGGEANQWYPTLYEGTTPVEPDKTPEEGYHLVDDMT
                     DKAIAWAGQQKALASEKPFFMYFAPGATHAPHHVPKEWADKYKGKFDEGWDKIREETF
                     ARQLKLGVIPPDSKLTERPKQIPSWDEMPEDLKPVLKREMEVYAGFMEYTDHHVGRLL
                     DSLKKLNILDDTIVYYIIGDNGASAEGTLNGTYNEMLNFNGLSALETPEFLNAKIDKF
                     GDPDSYNHYAVGWAHAMNTPYQWTKQVASHFGGTRNGTIVHWPKGIKGKGEMRNQFCH
                     VIDFAPTILEAAGIPEPISVDGVQQHPYEGVSMLYSFNDGSAPEQHETQYFEMFGNRG
                     IYHKGWTAVTRHKTPWLLMEEKTLPFDDDVWELYDTTKDWTQANDLSKEMPEKLHELQ
                     RLWLMEAVRYNVLPLDDDIAKKMNPDTAGRPILIKGDTQILFGGMGRLLENCVLNIKN
                     KSHAVTAEIEVPEAGAEGVIIAQGANIGGWSLYAKDGKLKYCYNLGGINYYYVESENK
                     IPAGQHQVRMEFAYDGGGLAKGGVVSLFVDGKKDGEGKVDMTLPMVYSADDGCDVGVD
                     SGAPVSEDYGPLGNRFNGKVRGVQLSITDDGKNSDHMVDPEEAVRIALSRQ"
     gene            13629..14372
                     /locus_tag="PLX80_07200"
     CDS             13629..14372
                     /locus_tag="PLX80_07200"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HQY20612.1"
                     /translation="MNRKKLLFFKSIFILGILFQSTSSFSQFDSMAVVTLDKMSDKIH
                     STNSVSFTSYVEYDKQSPNSGLITHSDMAKVYLKGPNKILISYSGDNGQKSYYYNGKT
                     FTYFSLTNNQYSIIDAPATNIETVDLLHNDYGMEFPAADFLYPNFVDDLIKHSKNLIY
                     LGVTDVNGKEVSHIAGAGIADKMTYQFWIYNDTFLPAMIKIVYIDQPGNPQYQNTFSN
                     WDIGPVLEDSLFEFRIPTDSKKVELKKSN"
     gene            14422..15237
                     /locus_tag="PLX80_07205"
     CDS             14422..15237
                     /locus_tag="PLX80_07205"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HQY20613.1"
                     /translation="MKTRSYIFILIISLMIIGIISETSYAQRFGRGGFSRGGGGFDRG
                     GGFGGNSMSGFDRGGMNGSRDGNFSNGNFNKGDFNNSDINRDNFNNTDINRNVQNNFY
                     GNNNIYHNGAYGYGGYNAYAYHPYHPYAYGSSWYAPGAMLTTMYASAVMLNVDNQSYY
                     YNEGVFYQPTDGGYTVVNAPSGAEVTLLPSGYEATNVGSETYYYYGGTFYQEEGAGYY
                     VVGAPVGAVIYNLPEGATSVTNSSSQQLLQYGGAYFQPITKDGQDAYEVVTMS"
BASE COUNT         4592 a         2847 c         2511 g         5372 t
ORIGIN      
        1 aattcgtaat tcgtaattat taattcctga ttgcttttta accgcatacc tgtttgaaca
       61 ctctcaaaaa attcagccct aaaatttttc tgatctcatc cggtgtatag cctttttcaa
      121 ataatctttt tgtaagttcg ggatactggg ttgcgtccct cagatcacct ggcgctgaga
      181 tccctccgtc gaaatcagaa ccaattccga tgtaatcaac tccgacaaga ttttttatat
      241 gatccatatg agcaattata tcttcaatac ctgcgccgta tttttttcct ccctgtacaa
      301 gaaattcatc ataaaaatta attccgatat atccttttga ttcagcaatt gctttgatct
      361 gttcgtctgt gagatttctg taatggggat ttaaagaata gcagttggaa tgtgaagcaa
      421 taaccggtgc agtggaaatt tcaatcacgt cccagaatgc agcttcgctt aaatgcgaaa
      481 catcgaccat cattcctgtt tcattcattt ttgtaattac ctcttttcca aattcggtca
      541 gaccaccgct ctttccccgt gtgacagcat ctttagctga ggtagcaatg agattggaat
      601 tattccatgt aagtccgatg taccttactc cggcatcaaa aaatctctgt acattttcgg
      661 gatcactttc gactgcagtg ccgccttcta ttccgatcag tccgcagatg tttcccgcat
      721 ttgtaatgcg gattatgtct tcatacgttt ttgcaaattc aaatccgccg ttactttcgt
      781 tttgaaaatt ctgcagtctg tcgatctgtg acattacaaa gctgtatgaa ctcttcacct
      841 tacccatcgg tatccagacc gcaaagacct gaatatccag accgccttct ctgaatttaa
      901 aaatatccga ttgtgtagat gtgttattta cattaaagtc agcgcctctt tcatagacct
      961 gccatatcag atcattgtgt gtgtccgcta acagcgcatc aaagtgcagc ttcacgggat
     1021 cattatcaat cgtactatcg gccatattat ttttatttgt tttatcagat ttgttaattt
     1081 cggaattatc atatttaata tcagtcccgc tcaaattcat acctgcctgg gttaaaccgg
     1141 aatttaattc cggcgtactg cttccgctaa aatttcccct gttcaaataa atgaccaccg
     1201 atacagccgc aactgagatc agtaacttat ataaaatatt atatcctttt ttactcattg
     1261 ctgaaaaaat aaaattctta atactcaaga tcatcttcaa agaaagatga cttgtctgta
     1321 tttatagcaa ctttagccat ttcaaaatct ttctccggta caaaaaattt tattggcctc
     1381 ttcaaagcca ttctgcttag ctctgtatta aaagtctcca ttgaaatcgc aatattaaaa
     1441 tctccggtta ctttaaaagg gatctcttca tctgtaagtt ttgccttgat aagatttgct
     1501 tccatagggt cataagttac atgaacttcg ataaagtctt ccatgaatta tttcccctga
     1561 ttaaaattca gcattggcat aagtttctga tcatatggtg cgatcacaaa tgaattgttt
     1621 ggtgagttta caagctcttt caatgtctca atatatttcc actgaagata tgaattactg
     1681 atagtactgt tgatgatcct ttgcgcttca gctacaccgg ttgcttctac agtttttctt
     1741 tcagcttcct gtctttcttt ctgaagtaca tacaccatct gctgagcacg ctgctcggct
     1801 gagatttttt catcgatagc ttcacgtact ttttgaggaa gctctacatt ccttaaaaga
     1861 actttctcta atataatacc tcttccttcg aaagaatttt caagttttct tgcaacgtca
     1921 tttacatagt catctctttt tgatgaataa atatcagtcg ctacaaaatt tacagctata
     1981 tctctgatcg cagttcttat tgccggctga attatctttg cttcataatc cacaccaata
     2041 ttcctgatca gttcaggcgt atcatccgca cttaatctga accagacagt tacatcgaga
     2101 attaatgtca gaccgtctga agaaagcgtc tggatgggat cacttttttt tactctgatc
     2161 tcactgtcgt cctgatcatt tactccgctc attgtataag cctgtgtctt tatgtcaagc
     2221 ttctgaactt caatcaaagg gtttataaaa tttaaaccgc ttctcaatac tttgtcctgt
     2281 acatttccaa aaaggatctg tactccggct tctccgggac cgatctgaat tatggaagca
     2341 aatagaattg caaggagaat tcctactccc actgctacgc gtaatgtgtt tagaattttt
     2401 tcaacctgaa gattttcttt gaattgtccc ttaagtactg taatgagaac aattaaaact
     2461 atcagaaata ttaaaaatga aatcatgata aattagaatt ttgattaaat taaaaatcca
     2521 gttcagcagc agcggtatag gcagtatcaa ttcctttttg aatttcatta ttcaaaacgg
     2581 tgatatgtcc cattttcctg ccgtgtctgg tttgcttttt tccatacata tgtaaataag
     2641 tattttcatt tgccagcacc acacctgctc ttttaagtct gctctctcct tctcttttcc
     2701 caagcagatt tatcataata gaatttgcag aagacctgtt ggtatttccc aatggaagat
     2761 ccattacagc tctgatatga ttttcaaatt gtgaagtata agtgccttca atagtatagt
     2821 gtcccgaatt atgaactcgt ggtgcaagtt cgtttacaag aaattcattg ccattcagaa
     2881 acatttcaat tcccattacc cctgtataat tcagctcagt tactattttt ttagcaatgt
     2941 catttacttt agattctatc tgaccttcaa actgtccgga cgaaattaca agctcgcaga
     3001 tgtgatcttt ctgaattgtc tccaccaccg gataaacttt tatttcacct gatgtatttc
     3061 tgacaacctg agttgcgatt tctttttcaa aatttacaaa tctttcacac atcagctcac
     3121 cgcgttcttt tagctcatta atgcctttcg aaatatcatc ttcagaattc acagtaaaat
     3181 ttcccttgcc gtcataaccc atcgttctcg atttcaatat taaaggatag ccaaattctt
     3241 ctgcgaattt ttttacatca gaaagatctt ttacctctgt aaattctgca acaggtatat
     3301 catttgcctg cagggttttt ttttgaatga atttatcctg tatcagttta atattttctg
     3361 aacacggaaa tactttttta ccggtatctt ctattgcttt taattttttg tgatctatga
     3421 attcattttc taaagttatg atatcacagt tctttgcaaa ttcaatcaac gtcttttcat
     3481 cattccagtc gccttcagtt tcaaattttg tgatctgacc tgcgggtgaa tttttttcat
     3541 ttgagagcac atgaaattca aatgcatatt tattgcactc ttctattatc attcttgcta
     3601 attgtccgcc gcctaatatt ccaatcttca cctgagtata tctgatttat tttttaattt
     3661 acaaataaat tatttgacca ataatgatct gtacagaacc ggatcttcaa ggatcttcat
     3721 agcttcaatt acctgcttat cataagtaag tgcagctttg atctgttctt cctcagtgat
     3781 gattctttta ttgatctcgt ctgtgatctt aagaattatt tcattctttg caagttcaag
     3841 ttctgtttct tcttcttttt ctgcctctga ttttacttta tcaagaagat ctgtcatttc
     3901 cgttccgtac tttttttctg aggcaagctc ttcaatttcc tttatcttct tttcaattcc
     3961 tgatgtatag ctgaattcct gctcagttaa aaaatccctg aattgtaaaa agaaagcttc
     4021 gtccggatat atagtggtga gtcccggatt ttttttaagg taatcatctg caaatttaaa
     4081 atacatatcc tttgccataa gtgcaaaatg gatctcgctt tcaggctcta ctgttacttc
     4141 aacatccggt gaaataccgc cgtatgcagt gacagtcctg ccgccaagtg ttttaaaatc
     4201 tgtctgatta tatttcgcgt catctataaa tacgccgtat ttattttctt tgaaataatt
     4261 tttttgctgg atccatctcc ccgaaggtgt ataatatctt gaagtagtga tcttcatctg
     4321 agcattttta tcaaggtccc gtatctgctg aaccagtcct tttccaaagg atttatttcc
     4381 tacgatcact cctctgtcca ggtcctgaat gccgccggct actatctctg atgctgatgc
     4441 agatttactg tttataataa ctgccactgg aatttcagct gatatcattg gttcatattt
     4501 tgaaaaatat tttttctcgg aatcttctct ctttcctttc gtaattacaa gcagactatt
     4561 cttctcaact atcttattca atattccgat tgctgcatct aaaagaccgc cgagattatc
     4621 tctcagatct ataaccagtc ctttaagttc tctttcactt ttaagggttt tgatcgcatt
     4681 ctctacttca ctttctgaaa tgtttgtaaa tctgtcaagc tttatataac ctatgccgtc
     4741 actctccggc ccgagatatc caaagtaaga tacatttttt aatatgatct cctgtcttaa
     4801 taatgtaaaa tcaataatgt cgtcatctct ttttattttt atatttacct ccgatccgat
     4861 cggacccctg acaaaacttt tcatattctt tacttcatct ttctcaagtc tcacaccgtc
     4921 aatttcaaga aaaatatcgc ctactctgag accttttttc tgagcttcat atccgttcat
     4981 aacttcagag atcaccacca tgctgtctcg aatgttcact gtaattccta ttcctccgta
     5041 tttaccggtt gtgatcagat caagctggtc acggctgtcc tcgtctacgt aattagtata
     5101 tggatccaga gtcgccagca ttccttctat acctgctgtc atgaatttat ccgcatcgat
     5161 ttcatccaca tagttaagtg caatttctct gtacacctga ccaaacaatt ccatgtttct
     5221 gttgatcttt ttataaatgt catcatcatt ttttagattg aatccgacaa ataaaaatat
     5281 acctgcgaga agtatcgccg gataaatttt tataagattt ctttttatgt tattcatttt
     5341 catttagtat tatacttatt ttttaaactt taaaacgtga tcctttacaa tattatgaat
     5401 ctccggtatt gatttttttc cgtctataac tacaaatctt tttttattgt ctcctgagat
     5461 tttcagatat ccatcaataa cctttttata ataagacaga tttttttgtt caattctgtc
     5521 ctcagtttta tttatagctt tttttctttt gacaaattcc tctgcactga tatttatcag
     5581 aaacgtaata cccgggttca ggtttcctga tgcaatttta tttactgaat aaatagtttt
     5641 cagatcgatc tttcctccga atccctgata agccgtggaa gaatcaaaat atctgtcaca
     5701 caatacataa taattctttt ttaatttagg aattataact tcctgtgtaa gctgatatct
     5761 cgatgcagaa aacaacagaa actcggttac atattccata ttaagatttt tcttgtcgag
     5821 aagtatgtct ctgatctttt ctgagatccc ggtcccgccc ggctctcgga cagaaattac
     5881 tttctttttc ttacttatta aaaattcttc aagcagtttt atctgagttg acttaccgct
     5941 gagatctata ccttcgaatg ttatgaacat taattaatta taataacagg tttgagctga
     6001 tttctgataa tgtatctaca ctttttaata caattctatt gtccataaaa ttcaatacgc
     6061 aatgatttat atttagcgca gacaatatat aggactgtat tttagtttta aattataaaa
     6121 ttgatgtact caatacaatt tcaaaattag tattattaaa tgttttacat aaatatccta
     6181 agtgtcctta aagttcgtga ctcagcgtta atgaaaagaa tgaaatatga ttttgcaagc
     6241 gaagcgaagc aatctctgca cttaatgcca aaaatttcaa cagagaataa tcattttatt
     6301 ttggaatcag aattctttta aattatagaa ctgtataaaa atcggatagc ttcactcgaa
     6361 tgaaatcggg ttcgctaagg caaatattta caaaaaaatc ccgtaaagaa attatcattc
     6421 tcagcggatt tttgttattc aaaatttcgt atttgtaatt tgaaatttgt aatttgaaat
     6481 tgtttaagga atattcagag agaattagac tgctctatct ttaagtacgc ttatctttca
     6541 attctctgat cagatagttt ttgtcttttt taattttgtc ttttaaaaat tcgaatacgg
     6601 ttttaaattt tatcatctct atataaattg aattgtcatc tgatttgatt atttccatta
     6661 gtccttcatt cttcaattct ttttctaatt ctgaaaaatc ataccagaga ttataatcaa
     6721 aatttttaca gaagtatgtt actttttgtc tgtgtaagtt attttccgta tcgatcagat
     6781 caccttcaaa aattttatca gatctgtaat cgacttcaag tagtttttcc aaataatgta
     6841 tttcagttga atttctgaat acaccgccga tggatgcaag ataaccgtcc ttctcaaaaa
     6901 cttcagccat aagtgaatct ccgaaggatg ctgtatcttt tggaatgaaa ttatcttttg
     6961 aaaaattatt tccaatcgca gtgaatgaga acagaccgtc ggaagttctt ttgtccggga
     7021 atttttgccg gataacttcg ggcagcatgc caatatttac agcaggactt tccggcatat
     7081 tgaaaatttc attccgtgta taacttaatg taaatgcggg aatacagata tttccacctt
     7141 cattctcaat ctctttaagc atctcacaga atttatcgat cctgatttca ttggttttac
     7201 caagtctgat aagtccgata aaatcaagat gtatgaatgc cggattgtct tttgtttttt
     7261 ctgctaaaag ttttgcgaaa ttattttgca tcagtggaat tgctgttcgg aaggattctc
     7321 tttatttgct tctttttaaa tctgtctgtg tattctattt tatcagctat aattattttt
     7381 gcaggaactt tgaaactttg caggttctca cggcagtatt tttttatctt agatctgaat
     7441 tcacctttat cctcatctct gatcattgta acctcagcgc agataatatt gccggttatg
     7501 ctgttcggct ctgaataaat aattgcttcc ccgacattgt caatttcttt cagcacggtt
     7561 tcaatctcta ccggataaac tttttcccct ccaatattta ttaactcaga tcttctgcct
     7621 aatattctga aaaattctcc ctgagtttcg acatgatcac cggttataaa ccagccgtcg
     7681 tctgaataag gtgtaggata attcagatat ccgagaattc ctgacggcgt tttgatatga
     7741 aggatatcat ctactattct taactcatat ccctctccgc caagttttac ccagagagaa
     7801 ttattgcttt gagacgctgt ccttaatatc ccgacttcca ttagtccata ggtttgctga
     7861 aattttatat cagggaaaat tatattaagt cttttcaaag ttccttccgg cataggctcg
     7921 gtaccgtaag tgattttctt cagactgctc agatcaaaat ctttatatgc ctcgctgata
     7981 agcagaagat tcagaaaagt aggtgataca ggcagaagtt ctactttatg tttttcaatc
     8041 tgacgacaaa taaattccgg agttctttct tttgtaaaaa caagcttacc gccatttgac
     8101 aaagtatgca gcattgtatt tacacctcca atgtgatcaa acaacagaaa attcagggta
     8161 gtcaaagcat aacgcctggt cttgaacttt tccagtaatg cagtaaagtc attgacagca
     8221 gcttttggtt cgcctgaagt accggacgag aaaagcacta ttcccggatg ttttctttct
     8281 cttaaaacat tataatattc atgatctgaa gattttcctg tttccgtaaa agtaatatta
     8341 ttattatcga tctctatcaa aagctcaacc attgagatat cataaattct ttttacatct
     8401 tccttggtct tggaatttac agggacaatt atacattcat tttctgctaa agctaaaaac
     8461 atcgatgttg attctaaaga aaaatcactt ttcaaagcaa ctacttttcc tttaatatca
     8521 gatatctgac ttatctcagc ttttcttttt tcaaaaagct ccagtaaagt ttgataactg
     8581 taaggctgat cattccagac aattgcagtc ttttgagaat tattcttgaa ttcttctgta
     8641 agaaaatcca gaaacatcag actcctccca tataaattac ctgaccggtt ataaaactgc
     8701 tgttatcatt tatcaaaaaa tctgtcatgt ttgtaatatc ttcaaattcc ataagacgct
     8761 ttactgtcat ttggtttaat aatttttcaa tcttttcatc aggcacattt tttatcagat
     8821 cggttttcat tggtgccaga cctacagcat ttacggttat attgtatgga gaaatttctt
     8881 ttgcaaggat ttttgtaaat gaatttaatg cagcttttga agaagcatat acagactctc
     8941 cttctatgtt aagccgtacg gcgactgtgg acatattaat tattctgccg gttttatttt
     9001 tcttcataag ttttaccgat tctctggaaa acaaaaatga acctgttaca tttgttttgt
     9061 aaatatcaat tattgttttt aggggagtaa gaaggaagtg attcattgaa gcaattccgg
     9121 cattgtttat taatacatct aatctgttaa attcttttct tatctgacga agagtttctg
     9181 agacagcagt ttcatctgcg acatctaatt gaaaatgtct gtaattttta ttttttatat
     9241 ctgatttatt tcggctgcaa ccgcagacta tacagttttt atttaaataa tattcagcta
     9301 aatattttcc aatccctttg cttgtaccgg ttataagtat aactttattt tttatatcca
     9361 atattacttt acgtttagct taatgtttaa aataatttct attcttcttt ctgaacatta
     9421 tttaaaagct cagatacata atctgacaat gtctttacag tcaggaaagg actgtttttt
     9481 cttgatagtg ctttctcatc tgctatcgta atactgatcc cggtttcgtc ctgaattttc
     9541 tcctcgacac ctacaattaa attaactaac ccaaaagaat caagtttact tttactgcca
     9601 taaagaacag tatctgcagc tactttaatt tttttgtcag aaggaagact ttcattaatt
     9661 tcttttatga cctcaaagat gagttcgttt attttgtttt gttctgtcat attttgtgat
     9721 ttttttacaa aatatatttt aatcaattat ttttcaatct actaatttat ttcatttaat
     9781 gaattttttc acctgcttaa aattcagact tgaaaataga ttttaaaaac ctttaaatta
     9841 atttcttaaa ctcccaatta agatcaatct taaagaattg taaataaata tttggttttg
     9901 gatcataata aaatatccag tatagcttta ttcgaaaaac gctggccggt cgcccttaca
     9961 attatcatag tgacttttct tctctggatg atccctgata gagtaagtat tgttccaaac
    10021 tggactttat atgtaatatg cgtcttcatg ctgataccta tagcagcaat tggtatatca
    10081 aaagcaaatc ccttatggat taaatcggag aaatatgtac ttctattttt ccttatcata
    10141 attggaatag gaaatttgct gaacctaagt aatctggtac aggatatgct ttataaatcc
    10201 ggtgaaataa gtggtctgca gcttcttact tccagtatca gtgtctggat taataacata
    10261 ttgatgtttt ctttgttata ctggcagatc gacagaggtg gtccggaagc tcgtttaaac
    10321 agcagctata aaaaagctga ctggtacttt tcagaagagt cagctcctga aacttttaca
    10381 cctaaaaact ggaaccctgt attcattgat tatttatttc ttgcattttc tacatccaca
    10441 gctttcagtg ccaccgattc tttacctttg actccaaaag caaaattact tatgatgtta
    10501 cagagtatag tatctctgac tataattgtt gttgtaggag cacgagcaat taatatactt
    10561 gggagttaat gttatgaaag tgtaataata aaaaatcaga aatacatttt tataattaat
    10621 tataataatt actttgactt acttattaaa atactaagaa attagaattt taaattttcc
    10681 cttaacatca aaaaatactt tctgcttctg aaataattta taccggataa aaatcatttt
    10741 aaataatttc cccgaaaagt ttttcgaatt aaactttgta aaattaattt ttttacggtt
    10801 tatttttcga atcaattttc agctgtttaa aaatttggta taacttaagt tcattcatat
    10861 ttattattat aaatatcctt ttaaaatttc cccttaattg aactttaatg taaaaactgt
    10921 atttattaaa atttttcttt taacaataaa ctatttcata aaccaaaaaa ataaatctat
    10981 tgagtacaaa aaataaaaaa caccggtcac atcttccgat tcccgaccag gcatacagag
    11041 gtttcattgc ctatgatggt aaggatcctg attcaaaatt tccacccatc cagactttac
    11101 tgccgcctga aggagcacca aatgttctgc taatattaat tgacgatgct ggattcggtt
    11161 cagcaagtgc ttttggcgga ccatgtaaca ctccaaatgc agaaaagctt gctgccggcg
    11221 gtttgaaact gaaccgcttt catacaacag ctttatgttc acctacaaga caggcaattc
    11281 ttacaggacg taatcatcat tcggcagcta tgggaagcat cactgaaact gcaaccggcg
    11341 cgccgggata tacatctgtt cgtccaaata caatggctcc acttgcaaag actcttaaac
    11401 ttaacggata ttctacagcg cagtttggta aatgtcatga agtaccggta tgggaaacga
    11461 gtccagtagg tccgttcgat tcatggccga gcggcggagg tggttttgaa tatttttacg
    11521 gattcatagg cggagaagca aatcaatggt atccgacatt gtatgaagga actactccgg
    11581 tagaacctga taaaactcct gaagaaggtt accatcttgt agatgacatg accgataaag
    11641 caattgcatg ggcaggacag caaaaagctc ttgcaagcga aaaacctttc tttatgtatt
    11701 ttgcacccgg agcgactcac gctccccatc atgtcccaaa agaatgggct gataagtata
    11761 aaggaaaatt tgacgaaggc tgggataaaa ttcgtgaaga aacttttgcc agacagttaa
    11821 aattaggagt aattcctcct gacagtaaac ttacagagcg gccgaaacaa attccttcat
    11881 gggatgaaat gccggaagat ctgaaacctg tgttgaaaag agagatggaa gtgtatgcag
    11941 gatttatgga atatacagat catcatgtag gcagacttct ggattcactt aaaaaactta
    12001 atatactgga tgatactatt gtctattata ttatcggtga taacggtgca tctgccgaag
    12061 gtacactaaa cggaacttac aatgagatgc tgaattttaa cggactatcc gctcttgaga
    12121 ccccggaatt cctaaatgca aaaatagata aatttggcga tcctgattct tataatcatt
    12181 atgcagtcgg ctgggcgcat gcaatgaaca caccatatca gtggacaaaa caggttgctt
    12241 cacatttcgg cggaaccagg aacgggacta tcgttcactg gcctaaaggt ataaaaggaa
    12301 aaggtgaaat gagaaatcag ttctgtcacg tgattgactt cgcacctaca attttagaag
    12361 ctgccggaat tcctgaaccg atctcagtgg acggtgtaca gcagcatcca tacgaaggtg
    12421 taagtatgct ttattcattt aatgatggca gcgcacctga gcagcatgag actcagtatt
    12481 ttgaaatgtt tggtaaccgc ggtatttatc ataaaggatg gacagcagtt acccggcata
    12541 aaactccatg gctattaatg gaagaaaaaa ctctgccttt tgacgatgat gtatgggaat
    12601 tatatgatac cacaaaagac tggactcagg caaatgatct ttctaaagaa atgcctgaaa
    12661 aacttcatga acttcagaga ctttggctga tggaagcagt gaggtataat gtactgccgc
    12721 ttgatgatga tattgcaaag aaaatgaatc ctgatactgc cggaagacct atacttatca
    12781 aaggtgatac acagatactt tttggtggaa tgggaagact gttagagaat tgtgttttga
    12841 atattaagaa taaatctcat gctgtaaccg ctgaaataga agtacctgaa gcaggagcag
    12901 aaggtgtcat tattgcacag ggtgcaaata taggaggctg gagtctttat gcaaaagacg
    12961 gcaaactgaa atattgttac aatctcggcg gaattaatta ttattatgtc gaatctgaaa
    13021 acaaaatacc tgccgggcaa catcaggtac gtatggaatt tgcttacgat ggaggcggtc
    13081 ttgctaaggg aggtgtggtt tctttatttg ttgatggaaa aaaagacggt gaaggtaaag
    13141 tcgacatgac tctgccaatg gtatattctg ccgatgacgg atgtgatgta ggtgtggatt
    13201 caggtgcacc ggtgtctgag gattatggtc ctcttggaaa ccgatttaac ggaaaggtca
    13261 gaggagttca actgtctata acagacgacg gaaaaaattc tgatcatatg gtagatcctg
    13321 aagaagcggt gaggattgct ctgagcagac aatagttgaa caattttaaa tattaaatat
    13381 taaattttaa atgttcggtg atttaaggtc attttaaaaa ttaacttcca gaatctgttt
    13441 tttgattttg gaagtttttt aatatggagc tcgtaaattt tctaaagtaa tattgcatct
    13501 aatcattaaa gggttttaat tagaaataaa attataaaga atttttcatt tcttatcatt
    13561 attttataaa tgcatttttc aaaattaaaa ttaattactt aacctgtttt aactaatcta
    13621 atccaatcat gaacagaaaa aaattacttt tctttaaatc gatctttatt ttgggaatct
    13681 tatttcagag tacatcttca ttttctcagt ttgactcgat ggcagttgta acattagata
    13741 aaatgagtga taagattcac agcactaatt cggtaagctt taccagctat gttgagtatg
    13801 acaaacagag tccaaattca ggactgataa ctcattctga tatggcaaag gtatatttaa
    13861 aaggtcctaa taaaatttta atttcttact caggagataa cgggcaaaag agttattatt
    13921 ataacggaaa gactttcact tatttttcct taacgaataa tcagtattct ataattgatg
    13981 cgccggccac caatattgaa acagtagatc ttttacacaa tgactacgga atggaatttc
    14041 cagctgctga ttttttatat ccgaattttg tagatgatct tatcaagcat tctaaaaatc
    14101 tgatctatct tggtgtcaca gatgtgaatg gaaaagaagt atcgcatatt gcaggtgcag
    14161 gtattgcaga taaaatgact tatcaattct ggatttataa cgatacgttc ttaccggcaa
    14221 tgataaaaat agtatatatt gatcagccgg gtaatcctca gtatcaaaac acatttagta
    14281 actgggatat tggtcctgta ttagaggatt ctttgtttga attccggatt ccgactgatt
    14341 caaaaaaagt tgaattaaaa aagtccaact aatttataaa tatattaatt aaaataattt
    14401 taaacttata ataatattat tatgaaaacg agatcatata tttttatact gataatttct
    14461 ctgatgatta tcggcattat ttcagaaaca tcttatgcac aaagattcgg acgcggagga
    14521 ttcagcagag gcggtggagg atttgacaga ggcggcggat ttggaggaaa tagtatgagc
    14581 ggctttgacc gcggaggaat gaatggttca cgggacggaa atttcagtaa tggaaatttc
    14641 aacaaaggtg actttaataa ttcagatatt aacagagata attttaataa tactgatata
    14701 aaccgaaacg tgcagaataa tttttacggt aataacaaca tttatcacaa cggtgcttac
    14761 ggttatggag gatataatgc ttacgcttat catccgtatc atccttatgc ttacggttca
    14821 tcatggtatg ctccgggtgc aatgttaact acaatgtatg catctgcagt aatgctgaat
    14881 gtcgataatc aaagttatta ttataatgaa ggtgtatttt atcagcctac tgacggcgga
    14941 tatacagttg tgaatgctcc ttcaggcgca gaagttactc ttctgccttc cggctacgag
    15001 gcgactaatg ttggttcaga aacatattat tattacggcg gcacgtttta tcaggaagaa
    15061 ggtgcgggat attatgtagt cggagctcct gtcggcgcag tcatatataa tcttccggaa
    15121 ggagcaacat cggtaacaaa cagcagcagc cagcagctcc ttcagtatgg aggtgcttac
    15181 tttcagccta tcactaaaga tggtcaggat gcttacgaag tcgtgactat gagttaaaaa
    15241 aaatgcaacc gggtttttag aaaagattca ggatatttca tttggattca tatgaatttt
    15301 acatgaaaat ttctgagtct tt
//
LOCUS       DAORVQ010000068        15310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1706_length_15310_cov_7.209046, whole genome shotgun sequence.
ACCESSION   DAORVQ010000068 DAORVQ010000000
VERSION     DAORVQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15310
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_15310_cov_7.209046"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..728)
                     /locus_tag="PLX80_07210"
     CDS             complement(135..728)
                     /locus_tag="PLX80_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HQY20614.1"
                     /translation="MKKNSKEALIIFAKNPVPGKVKTRLAADLGDDRAFEIYLKLLYR
                     AYANTKNLSCDKFLFLSDSKDDDLFDENFIQYLQKGNDLGEKMKNAFDEILNLGYEKI
                     LITGTDCPDLDENILTDAFEKLIEFDFILGPANDGGYYLLGMKSSADELFEDIEWSTE
                     KVLSETIKKIITDNKSYFLLRELIDVDNISDLDRSDL"
     gene            complement(725..1999)
                     /locus_tag="PLX80_07215"
     CDS             complement(725..1999)
                     /locus_tag="PLX80_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20615.1"
                     /translation="MSFYFFDISKEHKVILYIIVFGVNFVMYVILYLRNREIEFGKDD
                     VKWVLFAAIVLKLVLIMHEPVTSDDYYRYLWDGKVQSEGTDPFLYSPLELAEIHDEVI
                     YPNVSFPEIKTIYPPVSQIIFYAGYLISPHSTAGLKIIYLIFDIGIMLFLLDCLKKLH
                     LRSSAIILYAFSPLIILEFFLNAHIDIVLIFFLSGCIYFTINKNIPLSLLFLGLSILS
                     KTYSLMFLPILLLYLYRSEISLIRILKNILFFFLPFSIVYIYRDGIMNIFQVMGNYMT
                     HWYSNNLIYKIILFLSELTGFNDHAVTRKILLILSVISYFIIIFSKFEFVKKITFILI
                     FYFLFTHTVHPWYLCVLVMLLPLYLSYTSFYWTGIIALTNLTVFYYLSTGVWEDVFQV
                     MLIEYIGVMIFIFIDFKKIAKDRLLTQNEIII"
     gene            complement(2313..3008)
                     /locus_tag="PLX80_07220"
     CDS             complement(2313..3008)
                     /locus_tag="PLX80_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY20616.1"
                     /translation="MSEIYVIIPAINEEASIAKVINDIPENFVKEVVVIDNGSVDNTI
                     ETASNAGATVLTEPIKGYGAACLKGMDHIFAKCKDDDVIVFIDGDYSDYPEEMKKIAA
                     PVANENYDMVIGSRILGKKLGMTEEDSLLPQAEFGNWLSTALIKLFWKYEFTDLGPFR
                     AVKVSSLKKMKMADMNYGWTVEMQIKAAKLKMKCTEVPVRYRKRIGKSKVTGTVSGSV
                     KAGVKILYTIFKS"
     gene            complement(3001..4563)
                     /locus_tag="PLX80_07225"
     CDS             complement(3001..4563)
                     /locus_tag="PLX80_07225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY20617.1"
                     /translation="MQKKITDPFVINGLKILLGIIVALKFSISFNTGLFEDEAIYWNW
                     SLNPDPSYSFTTLTGIKIFTMMPGLLNEFTVRMAALLTNFVIIFFFYKTGMLLGVSDK
                     KILISMVLLFSIPFVTIYTSFISPDSMLLMFSVISIYFALRVIKSGSMTDWILCGITF
                     GFLILSKYTGAVFVPAFAVFLFLKRNEIPGITFSKIIAAVTSHLIIISPLIIWNIINE
                     PVWLNYYVNTGADAVSSGIGENLWKFILSQAAILLPVVFLFIIYITLKQISKKDKSVE
                     EKYLLFTGIFIFSVFAVLAMTGKLKGNWAFTAYIPLAISFLFINFTNFKKRNKFITAV
                     FTLMIIMNLFLLILLNLNSDSIKNIADNRFGNYINDTFHDYWPGHNDLAGNDKNWEDR
                     ILKMKQWQKNISEISNEINLIDIEYDFIASDNFNLSPLLKFYMKKDNVYLIGDLRFKY
                     INSAEVFENLSGKDAVIVTYKDSDVNYIYNRFEDVKEVKDIKFSISKDLIQEFRILQC
                     RNFKPEFVSAHE"
     gene            complement(4544..5341)
                     /locus_tag="PLX80_07230"
     CDS             complement(4544..5341)
                     /locus_tag="PLX80_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HQY20618.1"
                     /translation="MNKIFITLIFLLLLLISSCSEKETVPRVEPVEIDFDFVPVNNFF
                     KKYVKDGKVDYQSVKDDDALREFINQVKNTEPYNIPEGNERFAFWINAYNAFTIKLIT
                     DHYPLNSIVDIEEETGVNPWSINFIEMAGGRKFSLDEIEKEIIIPDYKDPRIHYALVC
                     AAESCPEIIPEAYLPEKLDAQLDLQAAIFLDDQDKNYLDKKENALYLSTIYKWYASDF
                     IKKDTTVTKHILKYINEGDKNFILENNTDDINYIDYSWKLNDAKENN"
     gene            complement(5357..6670)
                     /locus_tag="PLX80_07235"
     CDS             complement(5357..6670)
                     /locus_tag="PLX80_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQY20619.1"
                     /translation="MNEQLINIESPPEADHSVLKKTGLTLFSIGMLFFLISFSSVTDF
                     SPVLFFILAILLPCVGALIFFFAQLKGTLPGVRKNGIQFLSATNRGMLGWVTAVFLTG
                     MYILIYWFPELLENLIRIFDPLSYTLNGNPANQWFLYGTLYTLAVLVMGVRMIIQNRH
                     NKYQIIRTFSVMFFQLILAYLIPMFIQNGFYFTYFWPLKPEYLFPSSYSYLVSSGGLG
                     MFMFFWAAMMIFIATPVLTYFYGKRWYCSWVCGCGAMAETLGDPYRQNSDKSLTSWKI
                     ERWMVHSVLVIITVITAMLWIDNVNENFILGEFVHSANKWYAFFIGAMFAGVIGVGFY
                     PLMGSRVWCRFGCPMAAILGIFQKYFSRFRITVNGGQCISCGNCSTFCEMGIDVKSYA
                     QRGQNVIRSSCVGCGVCSEVCPRGVLRLENGPVKGRYELNKEYLR"
     gene            complement(6682..8022)
                     /locus_tag="PLX80_07240"
     CDS             complement(6682..8022)
                     /locus_tag="PLX80_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY20620.1"
                     /translation="MPENIVIIGNGITGITAARHIRKRSNSNIFVISSESKFFYSRTA
                     LMYVYMGHEKFEDIKPYEDHFWEKNRIELLFENANGIDTIKKQVNFASGNILNYDKLI
                     IAAGSISNKFGWKGQDLHGVQGLYSLQDLDLLEKNSRNIKRAVIIGGGLIGIELAEML
                     KSRNIEVTMFVREKSYWNSILPAEESEMINRHIREHHIDLRLDTELDQIKGDESQRVK
                     SIITKTGEEIECQLVGLTVGVRPNIDIIRNTDIETNRGILVNEFLETNIENVYAAGDC
                     AEFRTAFPNRKNIEQVWYTGRMHAEAIAETVCGIRTAYDPGIWFNSAKFLDIEYQVYG
                     LVNMKVEGEKNLYWECHKGNKSIRIVYTDECVIGFNLMGIRYRQEVCEKWIREKFRIE
                     KVLENLGEANFDPEFFSQYENELVQKFNSQHPGKNILMKRKRKLFNSLFNNSVQ"
     gene            complement(8041..8667)
                     /locus_tag="PLX80_07245"
     CDS             complement(8041..8667)
                     /locus_tag="PLX80_07245"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQY20621.1"
                     /translation="MLKKNKFQIIIVAIIIMLVVLLMSGKIVYPVKSNTLPLEVNDLH
                     EYMTPYFLNTSFSDEPAPEISNKGKWINTDKDITMNDLRGKVVLIEFWTFGCYNCTNT
                     LPYLKEWYDKYRSEDFEMIGIHCPEFDNERNYDNVKSSVEKLGIEYPVLTDNDFSVWK
                     KFDVHAWPTIFLIDKYGEIRYKKVGEGKYDKTERIIQELLAEKYSSAK"
     gene            complement(8701..9741)
                     /gene="arsS"
                     /locus_tag="PLX80_07250"
     CDS             complement(8701..9741)
                     /gene="arsS"
                     /locus_tag="PLX80_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183683.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HQY20622.1"
                     /translation="MLVKSLKFRDSELSIAGEQLNILNGYSDKAFSSYLKKSGIEKLT
                     SRKVEIFQINIGKMCNQTCKHCHVDAGPDRKEIMTRKTMQIIIDKLKDTGIKTADITG
                     GAPEMNPDFRWFVTELRKIDINTIVRCNLTIILANPKYNDLPDFFKENNIEVVSSLPY
                     FQKSNTDRQRGDGVFDASVKALQMLNEKGYGKEGSDLILNLVYNPTGAFLPGSQKSLE
                     NDFKKELLNKHGIVFNNLFTITNMPISRFLEYLIDSGNYEYYMDKLIDSYNPSAAENV
                     MCTNTISVGWDGYLYDCDFNQMLELKVENENKEHIRDFDLNYLDNREIITGQHCFGCT
                     AGAGSSCGGAVA"
     gene            complement(9757..10089)
                     /locus_tag="PLX80_07255"
     CDS             complement(9757..10089)
                     /locus_tag="PLX80_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HQY20623.1"
                     /translation="MENYYQSKDLKKFPDVSEFDEELAKKFFDYYGAVFAEGALTERE
                     KSLIALAVSHAVQCPYCIDAYSHDALEKGCNEKQMMEAVHVATAIRGGASLVHSVQMM
                     NHVKKVSM"
     gene            10374..12302
                     /locus_tag="PLX80_07260"
     CDS             10374..12302
                     /locus_tag="PLX80_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20624.1"
                     /translation="MSDDLNKPDSETPQDDESSKNSNDKDLNKKDTKDSEGSKIKNPD
                     EEPQGSIFSDGKEESISSDKTEKSSKDIEKDIEKDIEKSASGRDIRPFQPSTPSGKPK
                     ISNYQISLIDENYLKVSVATKKGIKINSSLIENTKYAEIQNNEIYLNPRIDLAQQEVE
                     IHITIDENSDKEIPDNEDKEITGSGELDDNILKDEDKESVESKFKVSPINENLLKVKI
                     ETDKSVKIDSVTSDKKTSLQTQSNRVLLLPDRNNKLHFIEITIELDSEIRDINEVISG
                     SSEDGKIKLIPNPEVVDLIQPPDYSEFPEDQGFLNPEGGFINPFDKLRHLIRRNLTIG
                     LVIAVMLHLVAAGIAFYTIGKKSDEQLPEEKPRLIVLQDLPDPKIKLQDVEDPNKPKI
                     EDIPADENPDNPVKREITPRKIVQPPRVKRPTDNSENDTLPASELAKELDSLRKLADA
                     LEGDTTDTLLDSLQSNYVIPDSLRNNFSEQDIGLAMYFPSNWKLTDQRDINKNEKEFK
                     GVVLTDTTAEQSGTMTMFIFLDSELKDFNAEDFKTEFAMIDTTLTGFVNEPKTIAGFT
                     DYRFYIFNKIGTEKLTLRASVRKQFFEQYKNEIESVVRSINIRKNINKTDSIKQDPPA
                     IPDESEEEVEGVEEDGTE"
     gene            complement(12504..13913)
                     /locus_tag="PLX80_07265"
     CDS             complement(12504..13913)
                     /locus_tag="PLX80_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20625.1"
                     /translation="MKKLILLSVFFFTFASGTNANIINVPSQHPTVQAGINASSNGDT
                     VLVQPGVYMENINFRGKKIVLTSLYYQTGNLSFIQSTVINGSNPQNPDTASCVIINNH
                     EDSTTVLQGFTLTGGTGTKWTDEHGAGLFREGGGLLIQYSNPVIQNNIITGNSAVTGG
                     VFSTGGGGVRIGDSYPRFLNNVVTNNTARYGAGVVLNYTGGEYKNNLIFKNYGSLDFG
                     AGTGVWVNGFFTRIISLINNTVAGNSAVSGNPGIYGNGGVVAVIRNNIVWGNTSPNNV
                     QIASGNFTVRYCNVQGGYNGAGNINVPPDFDTTNYYLKSTSACIDKGDSSIIYNDPAD
                     LINPSNAMWPARGTLRNDIGAYGGPGSNRIANTVVGINNLNSATGPLGFQLNQNYPNP
                     FNPKTKIIYELQVSNDELVSLKIFDVLGNEVAELVNEKQNAGNYSVIFDGSNFPSGVY
                     FYKLKTGQQSEVKRMMLIK"
     gene            14204..>15310
                     /gene="radA"
                     /locus_tag="PLX80_07270"
     CDS             14204..>15310
                     /gene="radA"
                     /locus_tag="PLX80_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQY20626.1"
                     /translation="MAKIKTKYVCQTCGYSSVRWSGKCPDCDNWNTLVEEIQSTDSRS
                     RTPLKNVKVSRGSYRLISEIDSVNESRTLTHISELDRVLGGGIVESSVILIGGDPGIG
                     KSTLMLQLAEKINDKKILYVSGEESAAQIKLRSDRLNYDLGNFYILSETNLDIINAVI
                     EIEIPDIVIVDSIQTVFRPEIESAPGTVSQLRESTALLMHLAKKNNFSIFIVGHITKE
                     GMIAGPKVLEHMVDVVLQFEGERTHSFRILRGIKNRFGSTNEIGIFEMTDMGLKEVLN
                     PSEVFLSQRNYGASGCVISASVEGTRPILIEVQALVTSSNYGIPQRTSMGFDYKKLNI
                     IVAVLEKKLGIFLNKSDIFLNIAGGIKIEEPATDL"
BASE COUNT         5060 a         2824 c         2581 g         4845 t
ORIGIN      
        1 ataacagtta aataatttca ccacaaagac actaagtcac aaagattaat attttttaac
       61 ttttttcctt tgtgtcttgg tgtctcagtg gtaagaacag atgttttgag acagtctcga
      121 gtgaggatgg tttactataa atcagatctg tcaagatcag aaatattatc tacatcaatt
      181 aattctctca aaagaaaata acttttgtta tctgtgataa tttttttaat ggtctctgac
      241 aaaacttttt cagtactcca ttcaatatct tcaaacaatt catctgcaga acttttcatt
      301 ccgagaaggt agtaaccacc gtcatttgca ggtcccagta taaaatcaaa ttcgataagt
      361 ttttcaaaag catctgtcaa aatattttca tcaaggtcag gacagtcagt tccagttatt
      421 aaaatctttt cataacccaa attcagaatt tcatcaaaag catttttcat tttttcacca
      481 aggtcatttc ctttttgtaa atactgtata aaattttcat caaagagatc atcgtcttta
      541 ctatcagaaa gaaacaaaaa tttatcacaa cttaaatttt ttgtattggc atatgcacgg
      601 taaagaagtt tgagataaat ttcaaaagct ctgtcgtctc caaggtctgc agcgagtctt
      661 gttttcactt tacccggtac gggatttttt gcaaaaatta taagagcttc tttagaattt
      721 tttttcatat aataatttca ttttgagtta ataatctatc ctttgcaatt tttttaaaat
      781 caataaatat aaagatcatt actccgatat attcaatcaa cattacctga aaaacatctt
      841 cccatacacc tgtactcaga taatagaaaa cagtgagatt tgtcagcgcg ataattcctg
      901 tccagtaaaa ggaagtataa cttaaataca gtggcagcag cattacaagt acacacagat
      961 accacgggtg cacagtatgt gtaaaaagaa aatagaatat taaaatgaat gtgatctttt
     1021 ttacgaattc gaacttcgag aaaattataa taaagtatga tatgacagaa agtataagaa
     1081 ggatctttct tgtgacagcg tgatcgttaa agcctgtgag ttcggacaaa aataaaatta
     1141 ttttataaat gagattattt gaataccagt gagtcatgta atttcccatc acctgaaaaa
     1201 tattcataat gccatctctg taaatgtaaa caatagaaaa cggcagaaaa aagaataaga
     1261 tatttttcaa tatcctgatc aaagaaattt ctgatctgta aagataaaga agcaatattg
     1321 gaagaaacat gagtgaatac gttttgctca aaatcgataa accgaggaat agtagagata
     1381 gcgggatgtt tttattaatt gtaaaataaa tacatcctga cagaaagaaa atcaatacaa
     1441 tatcgatgtg tgcattgaga aaaaactcaa gaattatgag aggtgagaaa gcataaagaa
     1501 taatcgcaga tgatcttaga tgtaattttt tcagacagtc taaaaggaaa agcattatac
     1561 caatatcaaa tatcagatat ataattttta aacctgccgt gctgtgaggt gaaatgagat
     1621 agccagcata aaatattatc tgagacacag gaggatagat ggttttaatt tcggggaaag
     1681 agacgtttgg ataaatgact tcatcgtgta tttcagcaag ttcaagcggc gaatacaaaa
     1741 acggatccgt cccttcactc tgcacttttc cgtcccagag gtaacggtag taatcatctg
     1801 atgtcaccgg ctcatgcatt atcagcacca gcttcaatac aattgcagca aacaaaaccc
     1861 actttacgtc atccttccca aattcaattt ccctgttcct cagatacaag atcacgtaca
     1921 tcacaaagtt tactccaaag acaattatat ataagatcac tttgtgctct tttgaaatat
     1981 caaagaagta aaatgacaac agcgatatta aatataaaaa caggcagagg acattgtatt
     2041 tgtctttgag aaaaatcaag tgtgtttata tttgttttac aaagttagga gtagagggaa
     2101 tcaatttcaa attacaaatt acgaattaca gatattaata aatggaaatc ctttaattct
     2161 gaaaatcccg gttcagacat tacggataaa tttcgggtgt attctcgcta aaaacatgcg
     2221 ggaatgaaat gtgggggagg acgagggaat gagaaatttt agggaacttg agttaatgac
     2281 ggaatgagtt ggtcatttac gaattacgaa tttcaagact taaagatagt atatagaatt
     2341 ttcactcctg ctttgacact gcctgagact gtgccagtga cctttgattt tccgattcgc
     2401 ttgcggtatc tgacaggaac ttcagtacat ttcattttga gttttgctgc tttgatctgc
     2461 atctcgaccg tccagccgta gttcatatct gccattttca ttttttttaa agaacttact
     2521 ttcacagctc tgaagggacc gaggtctgtg aattcatatt tccagaataa ttttatcaac
     2581 gctgtagaaa gccagttccc aaattccgcc tgcgggagaa gtgaatcttc ttcggtcatg
     2641 ccgagctttt taccgagtat gcgcgaaccg ataaccatgt cataattttc atttgcaaca
     2701 ggagctgcaa tttttttcat ctcttccgga taatcagaat aatctccgtc gataaaaact
     2761 attacatcat catctttaca ttttgcaaaa atatgatcca ttcctttcag acatgcagca
     2821 ccgtaaccct taatcggttc ggtcaataca gtagcaccgg cattgctggc agtttcgatt
     2881 gtattatcca cagagccgtt gtctattact actacttctt ttacaaaatt ttcgggaatg
     2941 tcatttataa cttttgctat ggaggcttct tcatttattg cgggaatgat aacatatatt
     3001 tcactcatgc gcagatacaa actcgggctt gaaatttctg cactgtaata ttctgaactc
     3061 ctgtatcaaa tcttttgaaa tggaaaactt tatgtccttc acttccttta catcctcaaa
     3121 cctgttgtat atataattca catcagaatc tttgtatgta actatcacag catctttgcc
     3181 acttaggttt tcaaaaactt cggcagaatt aatatatttg aaacgcaggt cgccgatgag
     3241 atacacgtta tcttttttca tataaaattt caaaagcgga ctgagattga aattatcgct
     3301 ggctatgaaa tcatattcga tatcaatcaa atttatttca ttgctgatct cggagatatt
     3361 tttttgccat tgcttcattt tcagaatgcg gtcttcccag tttttatcgt tgcctgcaag
     3421 atcattatgt ccgggccagt agtcatgaaa tgtgtcattt atatagtttc cgaatctatt
     3481 atcagcaatg ttttttatgg agtctgaatt taaattcaaa aggataagta aaaacaaatt
     3541 cataattatc atcaaagtaa agacggctgt gatgaattta tttctttttt taaaatttgt
     3601 aaaatttata aaaagaaaag atattgccaa aggaatgtat gcagtaaacg cccagttgcc
     3661 tttcagctta ccggtcattg caagaactgc aaatactgaa aagataaaaa ttccggtaaa
     3721 gagtaaatat ttttcttcaa cagatttatc ttttttactg atctgtttta aagtgatata
     3781 aattataaaa agaaaaacta caggcagtaa aattgcagcc tgtgaaagaa tgaatttcca
     3841 taaattttct cctatgcctg aagacacagc gtctgcaccg gtattcacat aatagttaag
     3901 ccagactggt tcgttaataa tattccagat tatcaatggc gagataatta tcagatgtga
     3961 agtgactgca gcaatgatct tagaaaaagt aattccggga atttcatttc tctttaaaaa
     4021 aagaaaaact gcaaatgcag gaacgaagac agctcccgta tatttactga gtattagaaa
     4081 tccaaacgta ataccgcaga gtatccagtc tgtcatactt ccggatttaa taactcttaa
     4141 tgcaaaataa attgaaatta ctgaaaacat taaaagcatc gaatctggtg aaatgaaaga
     4201 tgtgtaaatt gtcacgaaag gtatgctgaa taacaggacc atcgaaatca gaattttctt
     4261 atctgaaact ccgagcagca taccggtttt gtaaaaaaaa aatatgatca caaagttggt
     4321 aagcagcgca gccattctga ctgtgaattc attcaataat ccgggcatca ttgtaaagat
     4381 ctttatacct gtaagagtag tgaatgaata tgacggatcg ggattcagag accaattcca
     4441 gtagatcgcc tcatcttcaa agagaccggt attaaaagag atcgaaaact tcagggcgac
     4501 aattatgccc agtaaaattt ttaatccgtt tataacaaat ggatcagtta ttttcttttg
     4561 catcattaag tttccatgag taatcaatat aatttatgtc gtctgtatta ttttctaaaa
     4621 taaaattttt gtcaccttcg ttaatatatt tcagaatgtg ttttgttact gttgtatctt
     4681 ttttaataaa atcacttgcg taccatttat atatagtgga aagatataaa gcattttctt
     4741 ttttatcgag ataattttta tcctgatcat cgagaaatat tgctgcctga aggtcaagct
     4801 gagcatcaag tttttccgga aggtaagctt cgggaattat ttcagggcag ctctccgcag
     4861 cgcagacaag tgcatagtga attctaggat ctttgtaatc aggaataatg atctcctttt
     4921 caatctcatc gaggctgaac tttctaccgc ccgccatttc aataaaattt attgaccagg
     4981 gatttacacc tgtctcttct tctatatcaa caattgaatt cagaggataa tgatcggtaa
     5041 tcagctttat agtaaaagca ttataggcgt tgatccaaaa tgcaaatctt tcatttcctt
     5101 cgggaatatt atacggttca gtatttttga cctgattaat aaactccctt aaagcatcat
     5161 catctttaac actttgataa tcaacttttc catccttcac atatttttta aaaaagttat
     5221 ttacaggtac aaagtcaaaa tcaatttcaa ccggctcaac gcgcggcact gtttcttttt
     5281 ccgaacatga agaaattaaa agcaaaagta aaaatattaa tgttataaaa attttattca
     5341 tatataattt tgagagttat cttaaatatt ctttgttaag ttcatacctt cctttgacgg
     5401 gaccattctc taacctcaga acgcctctcg ggcagacttc cgaacagaca ccgcatccga
     5461 cgcatgaaga cctgataaca ttctgaccgc gctgtgcata agactttacg tcaattccca
     5521 tttcacagaa agtggaacag ttgccgcagg agatgcactg accgccgttg acagtaattc
     5581 tgaatcttga aaaatatttc tgaaagatac ctaatatagc cgccatcgga catccgaatc
     5641 tgcaccatac acggcttccc atcagcggat agaatccgac tccgatcact cctgcaaaca
     5701 tagcaccgat aaaaaatgca taccacttat tcgcgctgtg cacaaattca ccgagtataa
     5761 aattttcatt tacgttatct atccagagca tagccgtaat cacagtaata attaccaaaa
     5821 cagaatgcac catccacctt tcgattttcc agcttgtgag agatttatcg gaattctgtc
     5881 tgtaaggatc gccgagtgtt tcagccatcg caccgcagcc gcaaacccaa gagcagtacc
     5941 atctttttcc ataaaaataa gtcagtacag gtgtagctat aaagatcatc attgctgccc
     6001 agaaaaacat gaacatcccc agtccgcctg aacttacaag atagctgtaa cttgacggaa
     6061 agaggtattc cggtttaaga ggccagaagt aagtaaaata aaatccattt tgaataaaca
     6121 ttggaatcag atatgccaat atcagttgaa aaaacattac tgaaaaagtt ctgatgattt
     6181 gatatttatt atgtctgttt tgaatgatca tcctgactcc catcacgaga actgccagcg
     6241 tataaagagt cccgtaaagg aaccattgat tggcaggatt accgttaaga gtgtagctca
     6301 gcggatcaaa aattctgatg aggttttcta atagttcagg gaaccagtaa ataagaatgt
     6361 acatgccggt aagaaaaact gcagtcaccc atcctagcat tccacggtta gtagctgaca
     6421 aaaactgaat gccgtttttt cttacaccgg gaagagttcc ttttagctgc gcaaaaaaga
     6481 aaattaaagc acccacacat ggaagtaaaa ttgccagtat aaaaaaaagt acgggactga
     6541 aatcagtaac gcttgaaaaa cttatcagga aaaaaagcat accgatcgag aacaaagtaa
     6601 gtccggtttt tttgagaacc gaatggtctg cttcaggagg actttcgata tttattaatt
     6661 gctcattcat gtattctcaa tttattgtac agaattattg aacagtgaat taaaaagttt
     6721 tcgttttctt ttcatcagaa tatttttccc cgggtgctga gaattaaatt tctgaaccag
     6781 ttcattttca tactgactga aaaattccgg atcgaaatta gcttcaccaa gattttctaa
     6841 aactttttca attctaaatt tttctctaat ccatttttca caaacctctt gtctgtatct
     6901 gattcccatt aaattaaatc ctattacaca ttcatctgta tagacaattc tgattgactt
     6961 atttccttta tgacattccc agtataaatt tttttcacct tcgaccttca tattcacaag
     7021 accatagacc tgatattcaa tatcgagaaa cttggcagag ttaaaccaga tgcctggatc
     7081 gtaagcagtt ctaatcccgc agacagtctc agcaatcgct tctgcatgca ttcttccggt
     7141 ataccagacc tgttcgatat tttttctgtt gggaaaagcg gtacggaatt cagcacagtc
     7201 acctgcagca taaacatttt caatattcgt ttcgagaaat tcatttacta gaattccgcg
     7261 attagtttct atatcagtat ttcttattat atcaatattc ggtctaactc ctacagtaag
     7321 tcctacaagc tgacattcga tctcttcgcc tgtttttgtg ataatgcttt tgaccctctg
     7381 actttcatct ccttttatct gatcaagttc ggtatcgagt ctcagatcaa tgtgatgttc
     7441 acgaatatgt ctgttgatca tttcagattc ttcggcaggc agaatgctgt tccaataact
     7501 tttttccctg acgaacatag taacttcaat atttctggac ttaagcattt ctgccagttc
     7561 aattccgata agaccaccgc cgataatgac agctcttttg atattgcgtg aatttttttc
     7621 aagaaggtca agatcctgaa gagaatacaa accctgcaca ccgtgaagat cttgtccttt
     7681 ccatccgaat ttatttgaaa ttgaacctgc agcaattatt aatttatcgt aattgagaat
     7741 atttccgctg gcaaaattta cttgtttttt aattgtatca attccgttcg cattttcaaa
     7801 gagaagttca attctgttct tttcccagaa gtgatcttca tacggtttaa tatcttcaaa
     7861 tttttcatgt cccatataga catacatcag tgcagtcctt gagtaaaaaa attttgattc
     7921 ggaggagatc acaaaaatat tgctgttgct tctttttctt atgtgtcggg ctgctgtaat
     7981 acctgtaatg ccgtttccga ttataactat attttcaggc atatatagaa atttttaaaa
     8041 ttatttagcg gaagaatatt tttccgcaag cagttcctgt attattcttt ctgtcttatc
     8101 atactttcct tctccgactt ttttatatct gatctcaccg tatttatcaa taagaaatat
     8161 cgtcggccag gcatgcacat caaatttttt ccaaacagaa aaatcattgt cggtgagtac
     8221 gggatattca atgccgagct tttcaacact gctttttaca ttatcataat ttctttcgtt
     8281 gtcaaactca gggcaatgaa ttccaatcat ttcaaaatct tctgatctgt atttatcata
     8341 ccattctttg agatagggta aagtatttgt acagttataa catccgaaag tccaaaactc
     8401 tatcaacaca acttttcccc gcagatcatt cattgtgata tctttgtcag tatttatcca
     8461 tttacctttg ttggagatct ctggtgcagg ttcatcagag aaagaagtat tcaaaaaata
     8521 aggcgtcata tattcatgca gatcattcac ttccagcggc agtgtatttg acttcacggg
     8581 atatactatt tttccagaca taagcaaaac caccagcatt ataattattg caactataat
     8641 tatttggaat ttattttttt tcagcatatc aaaatattat ttaaagaaat aatagtaaaa
     8701 ttatgcaaca gcacctccac agctcgatcc tgcaccggca gtgcatccga agcaatgttg
     8761 accagtgatg atttctctgt tgtccagata attcaggtca aaatctctga tatgttcttt
     8821 gttttcattt tcaactttaa gctcgagcat ctgattaaaa tcacagtcat acagatatcc
     8881 atcccatcct actgagatag tatttgtaca cattacattc tcagcggctg aaggattata
     8941 agagtctatt agcttatcca tataatattc gtaatttccg gagtcgatca gatattcaag
     9001 aaatctgctg ataggcatat ttgtaatggt gaacaggtta ttaaatacta taccatgttt
     9061 attcaaaagt tcttttttaa aatcattttc gagagacttt tgtgaaccgg gcaggaatgc
     9121 acctgtcgga ttatatacga gattcagaat aagatctgag ccttcttttc cataaccttt
     9181 ttcattcagc atctgcaaag ctttgacaga agcgtcaaaa actccgtcgc ctctttgtct
     9241 gtctgtgttg cttttctgaa agtaaggaag ggaggataca acttctatat tattttcttt
     9301 aaaaaaatca ggaaggtcgt tgtatttagg attagccaat ataatagtga gattacatct
     9361 aactatggta ttaatatcaa tttttctaag ctcggtaaca aaccatctga agtcaggatt
     9421 catctcggga gctccgcctg taatgtcagc agtttttata ccggtgtctt ttaatttatc
     9481 aataatgatc tgcatagtct ttctagtcat aatttctttt ctgtcgggac cggcatctac
     9541 gtgacaatgt ttgcaggttt gattacacat ttttccgata tttatctgga atatttcaac
     9601 ctttctggaa gtgagttttt caatccctga tttttttaaa taagaagaga aagctttatc
     9661 ggaatatcca tttagaatat tgagttgctc accggcaatg gacagttccg aatctctgaa
     9721 tttcagactt tttacaagca tatcattaaa attaaattac atcgaaactt ttttcacatg
     9781 attcatcatc tgtacactat gaacaagcga agctcctcca cgaattgcag ttgcgacatg
     9841 aacagcttcc atcatttgtt tttcattgca gcctttttcg agtgcatcat gagaataagc
     9901 gtcaatgcaa tacgggcact gaacagcatg agaaacggca agcgcaataa gagatttttc
     9961 tctttcagta agagcacctt cagcgaatac agccccgtaa tagtcaaaga attttttggc
    10021 aagctcttca tcgaattcgg acacatcggg aaatttcttc aggtctttgg actggtaata
    10081 gttttccatt tataaaaaat ttttaagatt attaaaaaac agattttata ggtcttttaa
    10141 attaatttaa acagcatttc agacaacttt tataaaataa tataagttca tatattttct
    10201 cttaaattat attaaaataa atttttaata ttggaacaaa tttttaataa tagtataaaa
    10261 gggagtacaa tttaataagc aataaatata aaaataaaat accttctttt atagctattt
    10321 gaatttattg ttatgcttaa gttttctaaa ttacaccgat tttttaaaaa ttaatgtcag
    10381 acgatttaaa taagccggat tcagaaactc ctcaggacga tgaatcttcc aaaaacagta
    10441 acgataaaga tttaaacaaa aaagatacaa aggactcaga aggatcaaag ataaaaaatc
    10501 cggatgaaga gcctcaggga agtatatttt ctgatgggaa ggaagagagc atatcatcag
    10561 ataaaactga aaaatcttct aaagatattg aaaaagatat tgagaaagat attgagaaat
    10621 ctgcatcagg cagagacata agaccttttc agccatcaac accttccggt aaaccaaaaa
    10681 taagtaatta tcagataagt ctaatagatg aaaattatct gaaagtatct gtcgctacca
    10741 agaagggaat caaaataaat tcctctttaa ttgagaatac aaagtatgct gaaattcaga
    10801 ataatgaaat ttatctcaat cccagaatag atctcgctca gcaggaggta gagatacata
    10861 ttacaattga tgaaaattct gataaggaaa ttcccgataa tgaagataag gaaataaccg
    10921 gcagcggaga acttgatgac aacattctca aggatgaaga caaggaaagt gttgagagta
    10981 aatttaaagt cagtccaata aatgagaatc tgttaaaggt aaaaatagag acggataaat
    11041 ccgtaaagat agattctgtt acaagtgata aaaagacctc actacagact cagagtaaca
    11101 gagttcttct gctgcctgac aggaataata aattacattt tattgagatc acgatcgaac
    11161 ttgattcgga gatacgagac atcaatgaag taatatccgg atcttcagaa gacggtaaga
    11221 ttaaattaat tccaaatcct gaagtagtag atctaattca accgcctgat tattctgaat
    11281 ttcctgaaga tcagggtttt ttaaatcctg aaggaggatt cataaatccg tttgataaat
    11341 taagacattt aatcagaagg aatctgacca ttggtcttgt aattgcagta atgcttcatc
    11401 tcgttgctgc aggaatagct ttttatacaa ttggaaaaaa atcagatgaa cagcttccag
    11461 aggaaaaacc gagattaata gtgctgcagg atcttcctga tccgaaaata aaacttcagg
    11521 atgtagaaga cccaaataaa ccaaagatag aagatattcc tgctgatgaa aaccctgaca
    11581 atcctgtaaa aagagagata actcccagaa agatcgttca accacctaga gtaaaacgtc
    11641 caacagataa tagtgagaat gatactttgc ccgcatcaga acttgcaaaa gaattagatt
    11701 ctttaagaaa attagcagat gcgctggaag gggacacgac cgatacttta ttagattcac
    11761 ttcagtcaaa ctatgtcata cctgattccc tgagaaacaa tttcagtgaa caggatatcg
    11821 gacttgccat gtatttccca agcaactgga aacttactga tcaaagagat ataaacaaga
    11881 atgaaaagga atttaaagga gtcgtactta cagatacaac agctgagcaa tccggtacga
    11941 tgacaatgtt catttttctt gacagtgagc taaaagattt taatgcagaa gatttcaaaa
    12001 cggaatttgc gatgattgac accactctca ccggatttgt aaatgaacct aaaacgattg
    12061 ccggatttac agattatagg ttttacatat ttaataagat aggaacagag aagcttactc
    12121 ttcgggcatc agtaagaaag cagttttttg aacaatacaa gaatgagata gaatcagtag
    12181 ttagatcaat aaatatcaga aaaaatatta ataagacaga cagtataaag caagatcctc
    12241 ctgctattcc tgatgaaagt gaagaagagg tcgagggtgt tgaagaagac ggtactgaat
    12301 aggcagtttt ggagaatggt attgaggttt ggtttatgca gcaaaaacta acaggtttta
    12361 ccggaagaca ggaagtgtat cctgaatctg attaaacctg tcagttttcg tagccactgc
    12421 aaaactcaaa atttaattct ctacctgact cattaaacag ccttttcact tttccattaa
    12481 ccttttcgtg agtctgtaaa tccttacttt attaacatca ttctttttac ttcagactgc
    12541 tgacctgtct tcagtttata aaaataaact ccgcttggaa aattacttcc gtcaaaaatc
    12601 accgaataat ttcccgcatt ctgtttctca tttactaact cagcaacctc atttcccaaa
    12661 acatcaaata tcttcagact tactaactcg tcattcgaaa cttgtaactc gtatatgatc
    12721 tttgtctttg gattaaatgg attgggataa ttctgattga gctgaaaacc cagtggtcct
    12781 gtagctgaat ttaaattatt aatccccact actgtatttg caattctatt actgcccggt
    12841 ccgccataag cacctatatc gtttctgaga gttcctcttg ccggccacat tgcattagat
    12901 ggatttatca gatcagcagg gtcattatat ataatgctcg agtctccttt atctatacat
    12961 gctgatgtgc ttttcagata atagtttgtc gtatcaaaat ccggcggtac atttatatta
    13021 cctgcgccgt tgtatccgcc ctgtacattg cagtatctta cggtaaaatt tccgctcgca
    13081 atctgaacat tatttgggga tgtatttccc cagacaatat tatttcttat aacagcaacg
    13141 actcctccgt taccgtatat tcccggattt ccgcttacag cagaatttcc tgccacagta
    13201 ttattaatta aagagataat gcgtgtaaaa aatccgttga cccagacacc tgtgcctgca
    13261 ccaaaatcaa gcgatccata gtttttaaaa atgagattat tcttatattc tccgcctgta
    13321 taattcagca ctactcccgc tccatatctt gcggtattat ttgttacaac attatttaaa
    13381 aatctcggat atgaatctcc gatcctgact ccaccgccgc ctgtactgaa tacacctcct
    13441 gtaacagcag aatttcctgt tataatattg ttttgaatta ccggatttga atactggatc
    13501 aaaagtccgc cgccttctct gaacaaacct gcaccgtgtt catccgtcca ctttgttcct
    13561 gttccgcccg taagagtgaa tccctgcagg acagtcgtcg aatcttcatg gttgtttata
    13621 atcacacagc ttgctgtatc gggattttga ggattgctgc cattgattac tgtggactgg
    13681 ataaaggata aattaccagt ctgataataa agacttgtca atacgatctt cttcccacgg
    13741 aaattaatgt tttccatata aaccccgggc tgcaccagta ctgtatcacc gtttgacgaa
    13801 gcatttattc cagcctgaac tgtagggtgc tgtgacggta catttattat gttcgcatta
    13861 gtacctgatg caaacgtaaa gaagaaaact gaaagtaaaa ttaatttttt catttttgta
    13921 atatttaaat ttatgaacat ttatatatta gacagaataa aattacgttc gtgacaaatt
    13981 tttaaattgc cttatagttt aaaattataa acactgcaaa ttaaattatt tccccattct
    14041 caaatcccat gttttttaaa aatgaaacct gattaatatt ttatacaaac ttcattttaa
    14101 ttatctttga aaagtttttc agtaacttgc aaaagtactg tgatttctat tcgcataaaa
    14161 tttttagaaa ttttcggata atttaaaaat aaaatcgaac gacatggcaa agattaagac
    14221 taaatatgta tgtcagacat gcggctactc ttccgttaga tggagcggca aatgtccaga
    14281 ctgtgacaac tggaatacac tggtagaaga aattcaaagc actgacagcc gcagcagaac
    14341 tccgctcaaa aatgttaaag tcagccgcgg ctcatacaga cttatctccg aaattgattc
    14401 cgtaaatgaa tcacgaactc ttacacacat atctgagctt gaccgcgttc tcggaggcgg
    14461 tattgtcgaa agttctgtca tcctcatcgg aggtgatccg ggtattggaa aatctacact
    14521 aatgctgcag cttgctgaaa aaattaatga taaaaaaatt ctgtatgtaa gcggtgaaga
    14581 atcagcagcc cagatcaaac ttcgttctga cagactaaat tatgacctgg gaaatttcta
    14641 tatactttct gaaacaaatc tcgacatcat aaacgctgtc attgaaatag aaatacctga
    14701 cattgtaatt gttgattcta tacaaactgt tttcagacct gagattgaaa gcgctccggg
    14761 tacagtttca cagcttcgtg aatctactgc attactcatg caccttgcca aaaaaaataa
    14821 tttttcaata ttcattgtcg gacatattac aaaagaagga atgatagccg gtccgaaagt
    14881 tcttgaacat atggtggacg ttgtattgca attcgaaggc gaaagaactc attcattcag
    14941 aatactaaga ggaattaaaa acagattcgg ctctacaaac gagatcggga tttttgaaat
    15001 gaccgacatg ggtctgaaag aagttctcaa tccaagtgaa gtatttttgt ctcagagaaa
    15061 ttatggtgcg tcgggttgcg ttatatcagc ctcagtcgaa ggtaccagac caattttaat
    15121 agaagttcag gctcttgtta cttcatctaa ctatgggatt ccacagcgaa cttccatggg
    15181 attcgattat aaaaagctga acataatagt cgccgtactt gaaaaaaagc tcgggatatt
    15241 tttaaataag tcggatatat ttttaaatat tgcaggaggt attaagatcg aagagccggc
    15301 aacggatctt
//
LOCUS       DAORVQ010000069        15308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1707_length_15308_cov_6.460958, whole genome shotgun sequence.
ACCESSION   DAORVQ010000069 DAORVQ010000000
VERSION     DAORVQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15308
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_15308_cov_6.460958"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLX80_07275"
     CDS             complement(<1..572)
                     /locus_tag="PLX80_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQY20627.1"
                     /translation="MIKYIKLLLIIIVLFITSNLYSQSISGKITDKTNSEPLVGAVIK
                     ITGTIIGTITDLDGSFMLEAIDAGNYDLEISYIGYSPVTIKDVKVRDGENTKVNISLK
                     VDGLTTEEITVESSTTLSNEQSLLDAQKNSATIQDGISEQQINRAPDATTADVLKRVS
                     GVNIIDNKYVFVRGTSERYNNTTLNGVEVP"
     gene            891..1352
                     /locus_tag="PLX80_07280"
     CDS             891..1352
                     /locus_tag="PLX80_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20628.1"
                     /translation="MGTNFVKNGMGDHSYIKKLRTENDLLKKKLTEDFGMKHSEFNLD
                     PILENQWLKHILEFETTYASARKLKVYEIIGKPDFKKAETLNRTEIKMELDRITYLLA
                     QKNIHIENTTGQDPEKIYRFITEEIFEEVTQDMNIEGLNFCFVYEEPPAVN"
     assembly_gap    1367..1466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1592..3994)
                     /locus_tag="PLX80_07285"
     CDS             complement(1592..3994)
                     /locus_tag="PLX80_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY20629.1"
                     /translation="MIKIIFTFCFLITSNFVYSDNPKFHFELQNKINEASDNSTFPVY
                     ILLDEHLTLQDFSDIEYNTSKEERRKIVVDRLKNYSSNSQRNIRNFLNSKKSQRAIDD
                     FEILWITNSIITAANEDILNELTEFENVKMICYDATHPVEMLWDTKPIKAPFIGSDQM
                     QNSSSPPQPGITLMRADQVWALGNKGRGVIVANSDDGFWWKHPDLVNGIWQNLGEDAN
                     NNGMTVLIQSGTSSTFDPADINGVDDDGNGKVDDFIGWDYTTNNYNITTGSHGSGTLG
                     QVVGDGTGGTQTGVSPEAKAILLRNTAGESAQWAAFQYAVEMGAHVITSSLSWKWYFA
                     PKPNYSQMRLVTDMSLAAGVVHTNSTSNDGGSSSAPIPLNISTAGNCPPPWLHPEQIK
                     VGNVSGVIGVGNVTASSDIISTSSPYGPATWGNWNLWGTYTYPVDPGHMDYPYSRTSP
                     VELPDSMGLLKPDVSAPGNGTTSTSVSSGSGYSSFSGTSSATPHTAGCVALMLSVNPE
                     MLPADIDQVLELTSVEKGAPGKDPRYGTGRIDALAATTSPKFTLEGINGGNGYIINGV
                     LNPNDTAKELVGFKISTDVNPMIGSLKSMTLYKYGTADETHITSFDIYWDKDGNNLIN
                     TGDILLRSVPYSIEPIVFDSLKFKFNNIQRNLIVTARTTANVSNHFIYLELYGTSDVA
                     AYYTTTPFATNFPFSGPTGVENNNTVSVLNYSLSQNYPNPFNPATIINYTVAKDGDVK
                     LKVFNALGKEVAVLVNGFKTKGNYNVEFDSNDMGGLTTGIYYYKIESGNFSEVKKMIL
                     IK"
     gene            complement(4160..4882)
                     /locus_tag="PLX80_07290"
     CDS             complement(4160..4882)
                     /locus_tag="PLX80_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY20630.1"
                     /translation="MNYYFITGTGRGIGRALAEHLLQNENNFVYGLSRSNEISHENFQ
                     HIQIDLNKVNEVKNYEFPEVKNSESIILINNAASSGEVKHLGKRDQDLIISDYNVNII
                     SPSLIMNSFLKSYQNENCKRVILNVSSGAGKRAIESWSIYCSSKSALEMISEVTALEQ
                     KLKHSDNPVHIFAVGPGVVDTKMQDELRKVNPDDFSMVGQFIEFKEKGELADPKDIAI
                     KLTQIIADPDKFDKVSFSVKDF"
     gene            5432..6358
                     /gene="fabD"
                     /locus_tag="PLX80_07295"
     CDS             5432..6358
                     /gene="fabD"
                     /locus_tag="PLX80_07295"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQY20631.1"
                     /translation="MSKIAFVFPGQGSQTVGMGKDLFDNFDLAKEIYKKADEIMEMPL
                     STISFEGPADILTQTHITQPALFVHSYILIKLIGDKLIANTAAGHSLGEYTANVYAGS
                     FSFENGLRLVKKRGELMKVSGLRRPGTMAAVIGLSPEQVDEICEKAKEHQIVQPANYN
                     APGQIVISGDVDAVHRAMIIAKEEPYNSRMVKELQVSGAFHSELMLSSAEELKVALEQ
                     TEVYDSLIPVYANVDAEPVTEDDIIRNSLYRQLMSSVKWQGSIENMIRGLDVKKFYEI
                     GSGKVLTGLIKRINPDVEVFNIGTAEDIKNLS"
     gene            6418..7143
                     /gene="fabG"
                     /locus_tag="PLX80_07300"
     CDS             6418..7143
                     /gene="fabG"
                     /locus_tag="PLX80_07300"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQY20632.1"
                     /translation="MADFKGRVILITGGSRGIGKTIAESFSDEGATVIVTYRNSIDDA
                     YFDSKGIMHFKCDVSDMKSVQAMVDGIIKDHEKIDVLINNAGITKDGLLMRMSEDDWD
                     AVLDTNLKGVFNMTKAVTRNMMSKRYGRVINITSVVGITGNAGQANYVASKAGVIGFT
                     KSIAKELASRNITVNAVAPGFIETEMTDKLSDEVKQTYMNMIPLKKFGKGEDIAKAVK
                     FLAGGDADYITGQTLCVDGGIVM"
     gene            7227..7649
                     /locus_tag="PLX80_07305"
     CDS             7227..7649
                     /locus_tag="PLX80_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20633.1"
                     /translation="MNTKKQNAPTAAKIILTLLLGMVILSGCNNSFDALKGKVYLYKD
                     SVYEFTAAFEGDTIFYIMKDERMPMFHKSLFESEQVNDSTYLITLKDQPKFWDKNTWE
                     IVVVDDKKFRSKESGNYYNLYSDTMIVRLAPEFQKPVK"
     gene            7753..9276
                     /locus_tag="PLX80_07310"
     CDS             7753..9276
                     /locus_tag="PLX80_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQY20634.1"
                     /translation="MEIPAYLNDPKKIPDPGEFPFLRGVHNSMYRSKLWTMRQYAGFA
                     TAEESNERYKFLINSGTTGLSVAFDLPTQIGYDSDDPMSEGEVGKAGVAIDSLEDMET
                     LFEGIRLADITSSMTINSTAAILLMMYVAIAKKQNADLTKLSGTIQNDLLKEYIARGT
                     YIYPPKESMRLITDIFKWCSKELPSWNTISISGYHIREAGSSAVQEVAFTLSDGIEYV
                     KSAIESGLDVDDFAKRLSFFFNSHNNFTEEVAKFRAARRIWAKIMKERFGAKDDASMK
                     LRFHAQTGGSTLSDRQIDNNIVRVTIQALAAVLGGCQSLHTNGKDEAIALPTEQSVQT
                     ALRTQQIIAYESGVADSVDALGGSYLIEYLTDQIEEKAMDYINKIDEMGGASKAIEYN
                     YQKNEIEKNAYQLQKDIEDKKIIEVGVNEFMSDIEEEHIVFRVDDSVRTRQIEKLKIL
                     KEKRDNKKVNEILETIKKESQSDENLLPFILEAVENYATIGEISNALREVWGEYKAI"
     gene            9299..10009
                     /locus_tag="PLX80_07315"
     CDS             9299..10009
                     /locus_tag="PLX80_07315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY20635.1"
                     /translation="MRQKVIWVSGASSGIGREIAKEFSKAGHKVAVTARRKNDLAKLV
                     NEIRSEGGIASAFICNVMSGKSVISTFKKITEDLGKVDCLVNNAGTTVFKSFTDTSIS
                     DYDNVLNTNLRGAFLCIKSVLPEMLTKKKGHIINVLSVAANTAFENSSVYAASKAALL
                     AMSNSLRSEVRSKKIKVSNILPGAVETEMWDKKSIEQFGHKMMQPIDIAKIVLTVFNQ
                     PDRVLIEDIIVRPEFGDL"
     gene            10421..12409
                     /locus_tag="PLX80_07320"
     CDS             10421..12409
                     /locus_tag="PLX80_07320"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HQY20636.1"
                     /translation="MKIIFFISAFLFINFNINAEIIYTDPVQDAKYVSINNSIIIGFD
                     KQIKGSVINSVIQVRGSISGIHTGKFIITNDKMKLIFKPDLPFAFNESVEVNFIPDGS
                     GIGKSNRISYSFRTEVKKTKVDQNQILINELGNAPYNTNPGSLPLPTLQVLESNNPSP
                     GEIYLGSFAFAVGQSDSYLLISNNAGAYSYNRPSPNRFFDFRKQPNGMYTYFTVDKYY
                     GEDFNHNIIDSFYCGNGYTTDFHELVLLNNGHALLMSYDPQPVDMSQVVQGGNPNATV
                     FGLIIQEIDVNKDVVFQWRSWDHIAITDAIHENLLAASVDYIHGNAIEMDHDGNLLIS
                     SRHISEITKINRTTGDIIWRLGGVQNQFTFVNDPIKFSYQHDIRRLKNGNITLYDNGN
                     FHSPQFSRAVEYQLDEVNKVATLVWQFRRTPDYYGFAMGTTQRLPNGNTLIGWGAVSP
                     TLTEVRPNGQVALEMRLLWPTVSYRVFKDQTKLTLNLKLAIEGLYNSAEDRLNMKDTV
                     RAYIRNSTLPYNIIDSAVAYVDSVNFNGDFRFYNVPTGTYYVSTKHRNSIETWSKVGG
                     ESFVEYGVYHYDFTNLISKAFGNNLVLSGSKFCFYSGDVNQDGNIDLADGSIIGNDVS
                     VFAMGYLPTDINGDGIVDIIDALVTDNNVFDLIQKQTP"
     gene            12817..14868
                     /gene="secD"
                     /locus_tag="PLX80_07325"
     CDS             12817..14868
                     /gene="secD"
                     /locus_tag="PLX80_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQY20637.1"
                     /translation="MKKNLSRIVITIALIVLSIYFLYPTYQDYNLHKELSKLTGQDSV
                     DFIDKNAEELNDARDKRLKLGLDLKGGMYVVMDVDVVKLLEDMSTKKDETLTAILLEV
                     KEATTDNDEIILETFKVKLNEKGLNLKTYYGEIRDTDADVESRLKTEIDNALDRAVEI
                     VRNRVDQYGVAEPQIQKIGNSRIIVELPGVSNPEEVRKLLEGTALLEFKLIYDPQATV
                     RVMESINKIMTGDTTTVSDSTLSLNENDSLNSGNESITETDSSKLNSAATKDSLKEKK
                     STTENKLANKDTSVKKETDSVKKTDTSVTDADTATENDTLADETDTSDAQLSEEEFKE
                     KYPFFSMVSLNQESGTADGYVKESDKEKIERLFKREDIASLIPSDMQFAWSKKTFESE
                     GEKIYVLYAVKKDAELTGKVIVDARANIDPTSNTPVVTMEMDVEGASDWARITGANIN
                     KRIAIVLDNVVYSAPVVRSKISGGSSQIEGMADVPEAKLLEIVLKAGALPAPVKIIEE
                     RSIGPSLGEDSIRSGILSSIAALILVALFMIFYYKLGGSIADVAVIINMLFIFGIMAS
                     LKATLTLPGIAGLILSIGMSVDTNVLIFERIREEISLGKPLKTAVDLGYKKAFSAIID
                     SHLTGLITGVILYQFGTGPIQGFALTLIFGLLANLFTGIVITHYIIDILMEKGKKVSF
                     G"
     gene            14901..>15308
                     /locus_tag="PLX80_07330"
     CDS             14901..>15308
                     /locus_tag="PLX80_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20638.1"
                     /translation="MRIFENANYDFLGKRKIFYVVSIAIIVIGFTVLFTANSIPLGID
                     FSGGTEIQLQFEKDVNISDLRTAMDDAGFAGMEIKTMGTERDVLLRTPMQEEGQIVSD
                     KIQEGIKAKIGDNNFEVMRTDKVGPKIGEELRRN"
BASE COUNT         5231 a         2427 c         2793 g         4757 t
ORIGIN      
        1 ctcggcactt cgacaccgtt cagagtggtg ttattatatc tttctgaagt acctcttaca
       61 aagacatatt tattatcaat tatatttaca cctgatactc ttttcagaac atctgctgta
      121 gtagcatcgg gagctctgtt gatctgctgt tcactaatac cgtcctgtat agttgcggaa
      181 tttttctgcg catcgagaag cgactgttcg tttgacaaag tagtagaaga ttcaactgta
      241 atttcttcag tcgtcagtcc gtccactttt aatgaaatat ttacttttgt attttcacca
      301 tctcttactt ttacatcttt tatagttaca ggcgaatatc cgatataaga aatttcaaga
      361 tcataattac ctgcatcaat cgcttcgagc ataaatgaac cgtcaagatc ggtaattgta
      421 ccgatgattg tacctgtaat ttttataact gcaccgacga gcggttctga atttgtttta
      481 tcagtgatct ttccggaaat agactgtgag tataagtttg atgtgatgaa taaaacaata
      541 attattaata gaagctttat atatttgatc attattaaat ttaattatga acaaagatac
      601 ttatagcagt ttacgtgaag gttaatttaa agtaaagtat attaagatat tgttatgaaa
      661 ttgtaaagaa gtgaaatttt cttctaaaat cacagtaata tttttcatcc ccggataaat
      721 cccaattatt aatttcggac cgctcaataa atataaaaac ctaaaaccgg atatactcta
      781 attttcacaa actgctaaaa ttgcaatttg atataaataa atatctgtgt tatttttaca
      841 aagtaataag taactcagaa ttatttatta atcaaactaa aattaaaatt atgggaacga
      901 actttgtaaa aaacggaatg ggagatcatt cttacatcaa aaaattgagg acagaaaatg
      961 atcttcttaa gaaaaagctt actgaagatt ttggaatgaa gcactctgaa tttaatctgg
     1021 atccgatttt agaaaatcaa tggctgaagc atattttaga atttgaaact acatatgcaa
     1081 gtgccagaaa actgaaagtg tatgagatca tcggaaagcc ggattttaag aaagcggaaa
     1141 ctctcaatag gacagagatt aaaatggaac tggatcgcat tacatatctc ttagctcaaa
     1201 aaaacattca tattgaaaat acaacaggac aggatcctga aaagatttac agatttatta
     1261 ctgaagaaat atttgaggaa gtaactcagg atatgaatat tgaaggttta aatttttgtt
     1321 ttgtttatga agaaccgccg gctgtaaact gatctgatag tttgccnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnaaag tttgaggaga tattaattcc aaaattcact
     1501 tttagattcc caatcttttt gaatcaccaa cagcggctca aatatttaga aatcacaatt
     1561 tcaaaaatgg tgtcaaaatt acagcgaaaa attatttgat caggatcatc ttctttactt
     1621 cgctgaaatt tcctgattca attttataat aataaattcc agttgtcaaa cctcccatat
     1681 catttgaatc aaactctaca ttataatttc cttttgtctt aaaaccattt accaaaacag
     1741 caacttcttt tccaagagca ttgaaaactt tcaacttcac gtcaccatct tttgctacag
     1801 tataatttat tatagttgca ggattaaatg gatttggata attctgagac agagaataat
     1861 tcaatactga aacagtgttg ttattttcta ctcctgtcgg accggagaat gggaaattag
     1921 ttgcaaatgg tgttgtagtg taatatgcag ctacatcact tgttccatac aattctaaat
     1981 aaatgaaatg attggataca tttgctgtag ttcttgcagt tacaattaaa ttcctttgaa
     2041 tattattaaa cttaaatttt aaagaatcaa aaacaatagg ttcgattgaa tatggaactg
     2101 agcgaagtaa tatatctcca gtattgatca gattattccc atctttatcc caatagatat
     2161 caaaggaagt tatgtgggtt tcgtcagctg ttccgtactt atataacgtc attgatttga
     2221 gcgatccgat cattggattc acgtcagttg aaattttaaa acctacaagt tcttttgcag
     2281 tgtcgtttgg atttaagaca ccattgatta tataaccatt acctccattt attccttcaa
     2341 gagtaaactt cggagaagta gtagctgcaa gtgcatcgat ccttcctgtt ccatatcttg
     2401 gatcttttcc gggagcacct ttttccactg aagtaagctc aagtacctga tcaatatctg
     2461 caggcagcat ctcaggattt acagaaagca ttaatgctac acaacctgca gtatgcggag
     2521 tagctgaaga tgttccgctg aaactgctat aacctgaacc cgaacttact gaagtcgatg
     2581 tcgttccgtt tccgggtgca gatacatcag gttttaataa tcccattgag tcaggaagtt
     2641 cgactggact ggttctgctg taaggataat ccatatgacc cggatcaaca ggataagtgt
     2701 aagtacccca gagattccag ttgccccagg tcgcaggtcc gtatggtgat gaagttgaaa
     2761 taatatctga ggaggcagtc acatttccga ctccgatcac tccgctgaca ttgccaactt
     2821 ttatctgttc tgggtgaagc catggcggag gacaattacc ggcagttgaa atatttaaag
     2881 gtatcggtgc actggagctg ccgccgtcat tacttgtaga attagtatga acaacacccg
     2941 ctgcaagtga catgtctgtg actaatctca tttgtgaata atttggcttt ggagcgaaat
     3001 accatttcca gctcagagaa cttgtaatca catgcgctcc catttcgact gcatattgaa
     3061 aagccgccca ctgtgcagat tcccctgcag tatttctcag cagaatagct tttgcttcag
     3121 gtgaaacacc tgtctgtgta ccaccggtgc catcgccgac tacctgccca agtgtaccgc
     3181 tgccgtgaga acctgttgta atattataat tatttgtagt ataatcccat cctataaagt
     3241 catctacttt tccgtttcca tcgtcatcta ctccatttat atctgccgga tcaaaagtcg
     3301 aagaagttcc ggactgaatt aaaactgtca tgccattatt gtttgcatct tcaccaagat
     3361 tctgccagat tccatttaca agatcaggat gtttccacca aaatccatca tctgaatttg
     3421 cgactatcac tcctcgtcct ttatttccaa gagcccaaac ctgatcagct ctcatcagag
     3481 ttatcccagg ctgaggagga cttgaagaat tctgcatctg atctgaccca ataaatggag
     3541 ctttgatcgg ttttgtatcc caaagcattt ctaccggatg tgtagcatca tagcagatca
     3601 tctttacatt ttcaaactct gtaagttcat tcagaatatc ttcgtttgca gcagtaataa
     3661 ttgaatttgt aatccataga atttcaaaat catcaattgc tctctgagat ttttttgaat
     3721 taagaaaatt tctgatattt ctctgagaat tcgatgaata attttttagt ctgtccacta
     3781 caatttttct tctttcctct tttgaagtat tatattctat atccgaaaag tcttgtaaag
     3841 tcagatgttc gtcgagtaaa atatatacag gaaatgtaga attatccgaa gcttcattga
     3901 tcttattttg aagttcaaaa tgaaatttcg gattatctga gtaaacaaaa tttgaagtga
     3961 ttaaaaagca gaaagtaaaa ataattttta tcatgatact tattttaaat tttcattaat
     4021 gttaaatatc tgagtaatta tttcaagaga aaaaatgatt cgaataaaat tttgacaatt
     4081 taatccatta agtaattttc ctgagaaact tctttgtttc cgcagagtca cgtgctcttc
     4141 ggactctggg gatgcggaat taaaaatctt tcacactaaa acttactttg tcaaacttat
     4201 ccggatcagc aattatctgt gttaatttta ttgcaatatc tttcggatcg gcaagctctc
     4261 ctttttcttt aaactctatg aattgtccca ccatactaaa atcatccgga ttaacttttc
     4321 taagttcatc ctgcattttt gtatcgacta caccgggtcc gactgcaaat atatgaacag
     4381 gattatcgga atgtttcagc ttttgttcta aagcagtcac ttcacttatc atctccagtg
     4441 cagatttcga tgagcagtaa atgctccatg attcgattgc tcttttacct gcacctgaac
     4501 ttacattaag tatgaccctc ttacaatttt cattctgata actttttaaa aaactattca
     4561 ttattaagga tggactgata atattaacat tataatcact gatgatcaaa tcctgatctc
     4621 gcttgccgag atgttttacc tctcctgatg aagcggcatt atttattaag atgattgatt
     4681 cagaattttt tacttcggga aactcatagt tctttacttc atttacttta ttaaggtcaa
     4741 tttgaatgtg ttgaaaattt tcatgagaaa tttcattcga tcttgaaaga ccatagacga
     4801 aattattttc attttgcaaa agatgctctg caagtgctct gccgattcct ctgcctgtac
     4861 cggtgatgaa ataataattc attggatttt attttaaaga ataaattaat ttaatgggaa
     4921 taaattacag gtatctattt tcaagttcaa gttactcaaa tatttctcga attttatttc
     4981 atcaaatata aaaatgtcat tatttacttt cattgaaatt aaagcaatgt taagtttaaa
     5041 ataaattttc agagattttt aaaattattt tgaagacgac ttaatctaaa aaatattaat
     5101 aaaaataact gacacagatg taattaattt actgacagat attccagata agaatctgtt
     5161 caaaggtaag gtaggaacta ttgttgaaat tttatcagat tataaatatg aagttgaatt
     5221 ttgcaatcac aaaggtgaaa cagtttctat ggtactttta gatagcagta atttactttt
     5281 aatgcattat gaatttgaag ctgctaaatt ttaatttatt ccaaatttct tcattttacc
     5341 ttcacttcca ttctccaatg cttatcattc ctaaattgat ttaacacatt aaaataatta
     5401 atttgtctaa aattaattac acactatatt aatgagtaaa attgcattcg tttttcccgg
     5461 gcagggttct caaactgtcg ggatgggaaa agatcttttt gataattttg atctggcaaa
     5521 agaaatttat aaaaaggcag atgagataat ggaaatgccg ctgagtacta tttcttttga
     5581 aggtcctgca gatattctta cacaaactca cattacacag ccggcacttt ttgttcactc
     5641 ttatatattg atcaagctca tcggcgataa gcttattgcc aatactgctg caggtcattc
     5701 acttggtgaa tacacagcaa atgtttatgc aggttctttt tcttttgaga atggactgag
     5761 acttgtaaaa aaaagaggtg agctgatgaa agtttccgga cttcggagac cgggcacaat
     5821 ggctgctgtg atagggcttt cacctgagca ggttgacgag atctgtgaaa aagcaaaaga
     5881 acatcagatc gttcagcctg cgaattataa tgctcccgga cagatcgtaa tctcaggaga
     5941 tgtcgatgct gttcaccgcg caatgattat tgcaaaagaa gaaccttaca acagtagaat
     6001 ggtgaaggag cttcaggtaa gcggtgcgtt tcattcggag cttatgctat cttctgctga
     6061 ggaattaaaa gtcgcacttg aacagactga agtttatgat tcactgattc ctgtttatgc
     6121 aaatgtagat gcagagccgg ttacggaaga cgatataata aggaattcac tttacagaca
     6181 gcttatgtct tctgtaaaat ggcagggatc aattgaaaat atgatcagag gtcttgatgt
     6241 gaaaaagttt tatgagatcg gttcggggaa agttcttaca ggattgataa aaaggattaa
     6301 cccagatgtg gaagttttta atatcgggac agcagaggat attaagaatc ttagttaaat
     6361 tcacttctct ctaaaaaaaa taagagaatg ttcaaataaa ttttataaaa aataataatg
     6421 gcagatttta aaggcagagt aattttaata acaggcggat caagagggat tggaaagact
     6481 atcgctgaat cattttcaga tgaaggtgcg actgtaattg tgacatacag aaattccatt
     6541 gatgatgctt attttgattc aaaaggaata atgcatttta aatgtgatgt atctgatatg
     6601 aaatctgtgc aggcaatggt ggacggcata ataaaagatc atgagaaaat agatgtatta
     6661 ataaacaacg caggcattac aaaagacgga ctgctgatga gaatgagtga agatgactgg
     6721 gatgctgtac ttgatacaaa tctcaaagga gttttcaata tgacaaaagc agttacgaga
     6781 aacatgatga gtaaaagata cggcagggta attaatatta cttcagtagt cggaataacg
     6841 ggaaacgcag gacaggcaaa ttatgtcgca tcaaaagcag gcgtgatcgg atttacaaaa
     6901 tccattgcaa aagaacttgc atcaagaaat attaccgtga atgcagttgc acccggattt
     6961 atagaaacgg aaatgacaga taaattatct gatgaagtaa aacagactta catgaatatg
     7021 atacccttaa aaaaattcgg aaaaggtgaa gacattgcca aagcagtgaa attccttgca
     7081 ggcggcgatg cggattatat taccggtcag acgctttgtg ttgacggcgg aatcgtcatg
     7141 tagaatcagc ctacagactg attctgcaaa ttgaaaatta ataattaaaa attaataatt
     7201 aaaaatttga aattaattaa attaaaatga atacaaaaaa acaaaatgca ccaacagctg
     7261 cgaaaataat tttaacattg cttttaggaa tggtaatatt atccggctgt aataacagtt
     7321 ttgatgcttt gaaagggaaa gtatatttat ataaggacag tgtatatgaa ttcacagcag
     7381 catttgaagg agacacaatt ttttatatca tgaaagatga aagaatgcca atgttccata
     7441 agtctttatt tgaaagtgaa caggtaaatg attcaacata tcttattact ctgaaagacc
     7501 agccaaaatt ctgggacaag aacacatggg aaatagttgt ggtcgatgat aaaaaattcc
     7561 gatcaaaaga atccggtaat tattataatt tatattctga cactatgata gtaaggcttg
     7621 ctcctgaatt tcagaagccg gtaaaataaa ttccggataa ggacaaaaac tttttatagg
     7681 tgaaattaaa atgaatactg tttgaaaaag gaaattagat taaggaaaga aaattttgaa
     7741 accgattcag ggattgaaat tcctgcatat cttaatgatc cgaaaaaaat ccctgacccc
     7801 ggagagtttc cttttctgag aggtgttcat aactcaatgt acagatcgaa actctggacg
     7861 atgaggcagt atgcgggttt tgcgactgcg gaggaatcaa atgagagata taaatttctt
     7921 ataaattcag gtactacggg cttatcagtt gctttcgatc tgcctactca aatcggatat
     7981 gacagtgacg atccgatgag tgaaggagag gtcggtaaag cgggggttgc gattgattcc
     8041 cttgaagaca tggagacatt gtttgaagga atcagacttg ctgatattac atcatcgatg
     8101 acaataaatt ctactgcagc gatattgctg atgatgtatg ttgcaattgc aaaaaagcaa
     8161 aatgcggatc ttacaaaact ttccggcact atacagaatg atcttttaaa agaatacatt
     8221 gcgcgcggaa cttacattta tccgccaaaa gaatccatga gactgatcac cgacattttc
     8281 aaatggtgca gcaaagagct tccttcctgg aatacaattt ccatcagcgg atatcacatt
     8341 cgtgaagcag gatcgagtgc agtacaggaa gttgcattta ctttatctga cggaattgaa
     8401 tatgtaaagt ctgctatcga gtcgggactt gatgtagatg attttgcaaa acggctttca
     8461 ttctttttta attctcataa taattttaca gaggaagtag caaagttcag agcagccaga
     8521 aggatatggg ctaagataat gaaggaaaga ttcggagcaa aagatgatgc aagtatgaag
     8581 ctgcggtttc atgcgcagac aggaggctca acattgtctg acaggcagat tgataacaat
     8641 attgtgcgcg ttacaataca ggcacttgct gcggtacttg gcggttgcca gtctcttcat
     8701 acaaacggaa aggatgaagc cattgcactt ccgaccgaac agtcagttca gactgcacta
     8761 aggacacagc agataattgc atatgaaagc ggagttgcag attcagtaga cgcacttggc
     8821 ggttcatatc tgatagaata tttaacagat cagatcgagg aaaaggcaat ggattatatt
     8881 aataagatcg atgagatggg tggtgcatca aaagctattg aatataatta tcaaaagaat
     8941 gaaatagaga aaaatgcata tcaattacaa aaggacattg aagataaaaa aataattgaa
     9001 gtaggtgtaa atgaattcat gtctgacata gaagaagagc atatagtttt cagagtggat
     9061 gacagcgtta gaacaagaca gattgaaaag ctgaaaattc tcaaagagaa aagagataat
     9121 aaaaaagtaa atgaaatttt agaaactata aaaaaagaat cccagtcaga tgaaaatctt
     9181 cttcctttta tacttgaggc agtagaaaat tatgcaacaa taggggaaat ttcaaatgca
     9241 ctcagagaag tctggggcga gtacaaagcc atttaataaa tttcatattt ttaaaattat
     9301 gagacaaaaa gttatctggg tatcaggagc atcatcaggg attggaagag agattgcgaa
     9361 agaattttca aaagcgggtc acaaagttgc agttactgcc agacgcaaaa atgacttagc
     9421 gaaattagta aatgaaataa gatccgaagg tggcattgct tcagcattta tttgcaatgt
     9481 aatgtccggg aagagtgtaa tctcaacttt caaaaaaatc acagaagatc tgggaaaggt
     9541 cgattgtttg gtaaacaatg ccggcacgac tgtattcaaa tcatttactg ataccagtat
     9601 ttcagattac gataatgtac ttaatacaaa tcttcgcggc gcatttcttt gtataaaatc
     9661 tgtacttcct gaaatgctga cgaaaaagaa agggcatatt ataaacgtat tgtcagtagc
     9721 tgccaatact gcatttgaga acagttcagt atatgctgct tccaaggcag cacttcttgc
     9781 aatgtcaaat tctctgagat cagaagtacg gagtaaaaaa ataaaagtca gcaatatact
     9841 tcccggagct gtcgagactg aaatgtggga taaaaaatct attgaacaat tcggtcacaa
     9901 aatgatgcaa ccaattgata ttgctaagat agtattaact gtttttaacc agcctgacag
     9961 agttcttatc gaagatatta ttgtacgccc tgagtttggt gacctttaaa tttctgagga
    10021 taagttattt ttttgattat ggtttttgaa aaagtttaaa tatcaacgat attgaaatta
    10081 acctaaagac aaatatttat ttcggttata cattttataa taatctctat aataaaattt
    10141 attgaaaaaa caggtttttt tttcattaag tatgtttact catttttcat ttttgcttat
    10201 aaattgtata cacatctaaa ccacttaaac tcttgctcac actgcgttta tcaattattc
    10261 tccaaaattt aaaaaaatct caatttcgca tattgatttt tatattgtta aaaaataatt
    10321 agtagattat attacatcaa ttaaagatta acccaaatgt tcaccatatt aatcttaatt
    10381 ttattgttcc aaaattttta aattcaaaac ataactaatt atgaaaataa tatttttcat
    10441 ttcagctttc ctatttataa atttcaatat aaatgcagaa atcatatata ctgatcctgt
    10501 gcaggatgca aaatatgtca gcatcaacaa cagtataata attggatttg acaaacagat
    10561 caaaggatct gttattaatt cagttattca ggtgagaggt tcaattagcg gtatacatac
    10621 cggaaaattt attattacaa atgataagat gaaattaatt ttcaaacctg atcttccatt
    10681 tgcatttaat gaatcagttg aagttaattt tatccctgac ggttcaggta ttggtaaaag
    10741 caacagaatt tcatattcat tccggactga ggttaaaaaa acaaaagtgg atcagaatca
    10801 aatccttatt aatgaattag gaaacgctcc ctataataca aaccctggtt ctttgccatt
    10861 accaactttg caggttttag aatcaaacaa cccatctccc ggagagatct atcttggaag
    10921 tttcgctttc gctgttggtc aatcagattc atatcttctg atctcaaaca atgccggtgc
    10981 atattcttac aacagaccat cgccaaatag atttttcgat ttcagaaaac agccaaatgg
    11041 catgtacact tattttactg ttgataaata ttatggagag gattttaatc ataatataat
    11101 tgatagtttt tattgcggaa atggatacac tactgatttt catgaattgg tattattaaa
    11161 taacggtcat gctttactaa tgagctatga tcctcaacct gttgatatga gtcaggtagt
    11221 tcagggcggc aatccaaatg caactgtgtt tggtttgatc atacaggaaa ttgatgtaaa
    11281 taaagatgtg gtatttcaat ggagaagctg ggatcacatt gcaattacag atgctatcca
    11341 tgaaaatctt ttagcggctt cggttgacta tattcacgga aatgcaattg aaatggatca
    11401 tgatggaaat cttttaatct catcaaggca tatcagtgag attacaaaga tcaaccggac
    11461 taccggtgat attatctggc gtcttggtgg agttcagaat caatttacat ttgtaaatga
    11521 tcctattaaa tttagttatc agcatgatat aagaaggtta aagaatggta atataacact
    11581 ttatgacaat ggaaattttc actctcctca attttcgaga gcagttgaat atcagcttga
    11641 cgaagtaaat aaagtggcaa cattagtatg gcaattcaga aggacacctg actattatgg
    11701 atttgctatg ggtactacac agagacttcc aaacgggaat actttaattg gctggggcgc
    11761 tgtcagtcct actttgacag aagtaagacc taacggacag gttgctcttg aaatgagact
    11821 tctatggcca acggtatctt acagagtatt taaagatcag acaaaattaa cattaaactt
    11881 aaaacttgca atagaaggat tatacaattc ggcagaagac agacttaata tgaaagatac
    11941 tgtaagagca tatataagaa attctacttt accttacaac attatagatt cggcagtagc
    12001 ttatgtagat tcagttaatt tcaacgggga cttcaggttt tataatgtgc ctacagggac
    12061 ttattatgta tctacaaaac acagaaactc aattgagaca tggagtaaag tcgggggtga
    12121 atcctttgtt gaatatggtg tatatcatta tgatttcaca aatttaattt caaaagcatt
    12181 tggcaacaat cttgttttga gcggatcgaa attttgtttt tatagtggtg acgtaaatca
    12241 ggatggaaat atagatctgg ctgatggaag tattattgga aatgatgttt cagtttttgc
    12301 tatgggttat ttaccaacag atattaatgg agatggtata gtggatatta tagatgcctt
    12361 agttactgat aacaacgtct ttgatttaat ccagaaacaa actccatgat ctatattatt
    12421 tagatcataa atttaatatc cggatattag atccattttc tttaaaaatt attttacctt
    12481 agctaaaaat attcaaaaat tctagctaag gttttttatt tgtatcagga tacaatttgt
    12541 ttatttttta aatcattaac ttctatgttc agaaatattt ataaaatggt aaaagtaaaa
    12601 tttacaacag aaatttcaat tttgatttag caggaaaatt ttcaaagaat gtattacata
    12661 ataattttat cattagattt tatatagctg ttaaataaac aaatgaacag attgctctca
    12721 atattttaca ttgacatttc tcagcattag aattatattg aggactgtat tttttaaacc
    12781 tattcaagac attaacacat attacggagt gaaaatttga agaaaaatct ttcccgaata
    12841 gtaatcacta ttgcattaat cgtgctatcg atttactttc tctaccccac ttatcaggat
    12901 tacaatttac acaaagagct gtctaaactt acaggtcagg acagtgtgga ttttatagat
    12961 aagaatgccg aagagctaaa tgatgcaagg gataaaagac ttaaattagg tctcgaccta
    13021 aaaggcggta tgtacgttgt aatggacgtt gacgttgtaa agcttcttga ggacatgtca
    13081 accaaaaaag acgaaacgct gacagctata ttgttagaag taaaagaagc aacaacagat
    13141 aatgatgaga tcatcctcga aacattcaag gttaagctga atgaaaaagg attaaatctt
    13201 aagacttatt atggcgagat cagagacacc gatgccgatg ttgaatccag gctgaaaact
    13261 gaaattgaca atgctctcga cagagcagta gagattgtca ggaacagggt ggaccagtac
    13321 ggagtagctg aaccgcagat ccagaagata ggaaacagca ggatcattgt agagcttcct
    13381 ggtgtaagta accctgaaga agtaagaaaa cttttggaag gtactgcttt actagaattc
    13441 aaacttattt atgatccaca ggcaactgta agagtaatgg aatctataaa taagataatg
    13501 actggtgaca ctactactgt ttcagattcg actttgtctt taaatgaaaa tgattcttta
    13561 aattcaggca atgaatctat tacagaaact gattcttcaa aattaaactc tgctgcaaca
    13621 aaagattccc ttaaagaaaa gaaatcaact acagaaaaca aattagcaaa caaagatact
    13681 tctgtaaaaa aagaaacgga ttctgttaag aaaaccgata cttcagttac tgatgcagac
    13741 actgcaacag aaaatgatac tcttgcagat gagacagaca cttcagatgc acagctaagt
    13801 gaagaagagt tcaaagaaaa atatccattc ttctcaatgg tttccttgaa tcaggaaagc
    13861 ggtactgctg acggttatgt aaaagaaagc gataaggaaa aaattgagag actttttaag
    13921 agagaagata tcgcaagttt aataccatca gatatgcaat ttgcctggtc aaagaaaact
    13981 ttcgaatctg aaggtgaaaa aatatatgtt ttatatgcag taaaaaaaga tgctgagctc
    14041 acaggaaaag tcattgtaga cgccagagca aatattgatc cgacgagcaa tactccggta
    14101 gttacaatgg aaatggatgt agaaggtgct tcagactggg cgagaataac cggtgcaaat
    14161 ataaataaaa gaattgcgat cgtacttgat aacgttgtct attcagcacc tgtcgtaaga
    14221 agcaagatca gcggcggtag ttcacagatc gaaggaatgg ctgatgtacc agaagcaaaa
    14281 cttttagaaa tcgtattaaa ggcaggtgct ttacctgctc cggtcaagat tattgaagaa
    14341 agatctatcg gaccatcact tggtgaagat tcgatcagat caggtatttt atcttctatt
    14401 gcagcactaa tattagttgc actctttatg atattttatt ataaactcgg cggatcaatt
    14461 gccgacgtag ctgtaataat caatatgctt tttatatttg gtatcatggc ttcattaaaa
    14521 gcaacactga ctctacctgg tattgcggga cttattttat ccataggtat gtctgtagat
    14581 acaaacgttc ttatctttga aagaataaga gaagaaattt cactaggtaa acctttgaaa
    14641 actgcagttg atcttggtta taaaaaggca ttttctgcaa tcattgattc acaccttaca
    14701 ggtcttatta caggtgtaat tttgtatcag ttcggaaccg gaccaataca gggttttgct
    14761 ttaacattaa tattcggtct gcttgcaaac ttattcacag gtattgtaat tacacattac
    14821 attatagata tactgatgga aaaaggcaaa aaagtcagtt tcggttaatt attaaaaaat
    14881 aaaatttatt tgaatttata atgagaattt ttgaaaacgc taattacgac tttttaggta
    14941 aaaggaaaat tttttatgtg gtttccattg ccataatcgt aataggtttt actgtattat
    15001 ttactgcaaa cagtattccc ttagggattg atttcagtgg aggtacagaa atacaattac
    15061 aatttgaaaa agatgtaaat atttcagatc tgaggacagc tatggatgat gcaggctttg
    15121 cagggatgga aattaaaaca atgggaactg aaagagatgt tttgttgaga actccgatgc
    15181 aggaagaagg tcagattgtc tctgataaga ttcaggaagg tataaaagca aagataggtg
    15241 acaataactt tgaagtaatg agaactgaca aagtaggtcc aaaaattgga gaagaactca
    15301 gaaggaat
//
LOCUS       DAORVQ010000070        15267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1715_length_15267_cov_5.652314, whole genome shotgun sequence.
ACCESSION   DAORVQ010000070 DAORVQ010000000
VERSION     DAORVQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_15267_cov_5.652314"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLX80_07335"
     CDS             complement(<1..432)
                     /locus_tag="PLX80_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HQY20639.1"
                     /translation="MFSNLKQSIILTIFAIGIFGFAYPLFIFAIGNLIPEKSEGSPVY
                     INGKITGFENIGQNFSDDKYFNGRPSAVGYNAASSGGSNKGPTNPDYLAEVQARIDTF
                     MVHNPDVNKKEIPSELVTSSGSGLDPDISPDAAYIQIPRISK"
     gene            complement(514..2568)
                     /gene="kdpB"
                     /locus_tag="PLX80_07340"
     CDS             complement(514..2568)
                     /gene="kdpB"
                     /locus_tag="PLX80_07340"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HQY20640.1"
                     /translation="MSNHNKAANKLFQKELLKAALIESFVKLNPKVMVKNPVMFTVEI
                     GTIVMIVITVMLAFTPDAAQGSFLYNFLITLILFLTVLFANFAESIAEARGKAQADSL
                     RKTRQDTPANLLINGGNIKIIMSSELKKGDIILVSEGEIIPSDGEIIEGIATIDESAI
                     TGESAPVIREAGGDKSGVTGGTKVLSDKIKVLISIEKGESFLDKMILLVEGASRQKTP
                     NEIALIILLSGLTMMFLIVTVALEPFGRFSNTPIAIASLVALFVCLIPTTIGGLLSAI
                     GIAGMDRALRANVIAKSGKAVETAGDIDTLLLDKTGTITIGNRKATNFFIVNGMEENV
                     FIKYCALSSLADETPEGKSIIELAKKSGVEINNIEHRDDHFFKFTAQTRMSGIDLKDG
                     TIVRKGAFDVIKNLIESKNEIVPEKMISVVKKIAEDGGTPLAVTVNGKPAGVIQLEDI
                     IKTGIQERFHRLRKMGVKTVMVTGDNPLTAKFIAKIAGVDDFIAEAKPEDKMNYIIEE
                     QRSGKLVAMMGDGTNDAPALAQADVGVSMNSGTQAAKEAGNMVDLDNDPTKLIEVVEI
                     GKQLLITRGAITTFSIANDVAKYFAIVPALFIASIPQLQALNIMNLTSPQSAILSAVI
                     FNAIIIPMLVPLALRGVVYKPVGAEKLLAGNLLKYGLGGIIVPFIAIKLIDVVLTVLH
                     IV"
     gene            complement(2581..4281)
                     /gene="kdpA"
                     /locus_tag="PLX80_07345"
     CDS             complement(2581..4281)
                     /gene="kdpA"
                     /locus_tag="PLX80_07345"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HQY20641.1"
                     /translation="MNTEILSVLFIFLLIFILGIPFGKYISKVFKNEKTLLDFLAPLE
                     NLIFKFSGIDPDKEMDWKQNIKAMLTINFIWFIWAILVLSFQNLLFWNPDNIPGMEPT
                     QAFNTAVSFMTNTNLQHYSGETGVSYLSQLIVMCFLQFVSAGTGIAVAALMFKGLANK
                     SGTNLGNFYNLMLKSCTRILLPVSVVVALILMFNGIPMTFKGAENIVTLQGDTVSVAR
                     GPAAPMVGIKQLGTNGGGFFGTNSAHPFENPNFFTNIIELVSILFIPVAMIIALGYYL
                     NRKKLSYMIFGVMLTGYVCFLIPAIYFEMNGNPEIDKMGISQPLGNMEGKEVRFGSAS
                     SAMWGVSTTVTSNGSVNSMHDSFTPITGMILMLGMQLNAFFGGVGVGFLNKFVFIILA
                     VFISGLMVGRTPEFLGKKIEGKEIKIAIIVALMHPLLILAGTAIAAHIWLITPIQDNF
                     LNNPSYHGFSEMFYEFSSAAANNGSGFEGLIDNTPFWNISTGIVILLGRFIPIIGPVA
                     IAGLLSAKKFTPESAGSLKIDSPIFGIVLLSTIVILGALLFFPGLALGPVSEFFSMAK
                     "
     gene            complement(4632..5975)
                     /locus_tag="PLX80_07350"
     CDS             complement(4632..5975)
                     /locus_tag="PLX80_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY20642.1"
                     /translation="MAFDKNLLIVDDERNLRNLLERILSLEGYSVTSCETSKEGLTAI
                     ENNLFYVALIDVILPDYNGIQLSKEIKEKSPDTEVIVMTAFANIRDGLEAVRNGAFDY
                     IEKDKDEDEIILKVKQAYDKAILKIKLKELQNKIDRNFSFNSITGKAKSISGAIELAK
                     KVADTDTTVLLLGETGTGKELFAQAIHESSSRKNKPFIAINCSAIPKDLQESELFGYV
                     KGAFTGAVKDKKGYFEAADGGTIFLDEIGDMSLETQSKFLRVLETNSLTKVGDTKTTH
                     VDVRVISATNKDLKSEIEKVNFKNDLYYRLNTFTIYLPALRERKDDLELLINNFIGNY
                     NDKLKKNISKASEDFISGLKEYSFPGNIRELKNLIERAVILCKGSELMAEDLPEEIYQ
                     HKFPVEIQSDSLKEIEKQHILKIYSENKHNKSITAEKLGIGAVTLYRKLKEYGVD"
     gene            6375..6890
                     /locus_tag="PLX80_07355"
     CDS             6375..6890
                     /locus_tag="PLX80_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HQY20643.1"
                     /translation="MNTENIISSDIGLIHLIASVLALITGSLVLSLTKGTRLHKKIGF
                     TYVTSMTILLITAFMIYRLFGTWGVFHWTALISSLTLLSGIIPILIKRPVKNYILYHF
                     SFMYWSVIGLYGAFAAETLVRLPKIVIESGIPNKVFYNMTGIAVGLTMGIGAYFFIKL
                     KSKWETQFARK"
     gene            7414..8997
                     /gene="aceB"
                     /locus_tag="PLX80_07360"
     CDS             7414..8997
                     /gene="aceB"
                     /locus_tag="PLX80_07360"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HQY20644.1"
                     /translation="MPENIQITANLKEKYKEILTEEAIIFISDLQKKFNSRRKELLAN
                     REVIQNEIDSGKMPDFPEETRSIRESDWKVAEIPDDLKDRRVEITGPVDRKMIINALN
                     SGANVFMADFEDSNSPTWENLIEGQINLRDAVNKKISFTNPEGKEYKLNEEVAVLMVR
                     PRGWHLEEKNILLDNENFSASLFDFGLYFFHNAKDLISKGTGPYFYLPKLENYLEARL
                     WNDVFNFSQDELGIPRGTIRATVLIETILAAFEMDEILYELKDHSAGLNCGRWDYIFS
                     FIKKFRNYNDYVLPDRSRVTMNVHFLRSYSQLVIKTCHRRGTHAIGGMAAQIPIKNDE
                     KANEEAMSKVKSDKEREAGDGHDGTWVAHPGLITTAKEIFDRLMPAQNQIDNKREDVK
                     ISAADLLTVPKGEITEEGLRHNINVSIIYLEAWLNGNGCVPIYNLMEDAATAEISRAQ
                     IWQWIHCENGILNDGRKVTSDLYHELVTDELKKIKNLVGEKRFSEGKFVKSTELFNEL
                     STDREFCEFLTLTAYNHIK"
     gene            9014..10297
                     /gene="aceA"
                     /locus_tag="PLX80_07365"
     CDS             9014..10297
                     /gene="aceA"
                     /locus_tag="PLX80_07365"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HQY20645.1"
                     /translation="MNKTDQINQIKNSWASGERWIGIKRPFSAEEVYKLRGSVQIEHT
                     IAKLGAERLWDLLHTEKFIRALGALTGNQAVQQVQAGLKAIYLSGWQVAADANNAGQT
                     YPDQSLYPADSVPSVVKRINNALMRADQISHMEDKNDVHWFAPIIADAEAGFGGNLNV
                     FELMKGMIEAGAGGVHFEDQLSSAKKCGHLGGKVLVSTREFINTLIAARLAADIMGVS
                     TILIARTDANSANLLLNDIDERDRKFIHGERTIEGFYKINGGLECAIDRGLSYAPYAD
                     LIWCETAHPDIEEAKTFADAIHEKFPGKMLAYNCSPSFNWKRNLNDNQIAEFQEKLGE
                     LGYKFQFITLAGFHALNTTMFQLALDYKSAGMTAFTKLQELEFDLEKTSGYKAVKHQS
                     FVGTGYFDQVQVSISSGQSSTIALEGSTEAEQFIK"
     gene            complement(10324..10464)
                     /locus_tag="PLX80_07370"
                     /pseudo
     CDS             complement(10324..10464)
                     /locus_tag="PLX80_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(10546..10806)
                     /locus_tag="PLX80_07375"
     CDS             complement(10546..10806)
                     /locus_tag="PLX80_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HQY20646.1"
                     /translation="MKVTVWDTYVTKQDVTIIHFDIIAPEEVKDQSVIQNYGKEYLKI
                     KGKDDQTLTSNECKRCHVEEIRPKWKSEIIEKGYYIVEMENC"
     gene            11109..12587
                     /locus_tag="PLX80_07380"
     CDS             11109..12587
                     /locus_tag="PLX80_07380"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQY20647.1"
                     /translation="MTRLLLISFILLFTSVNCKSNNSTDRKLPDNNFKNDEPRITDIT
                     LKNSTPQENLIESGPIVGAVTENSATFLVKPVNSSSVRIQLSEDSSFTEPFYTEEINT
                     DQNNFNFTKIQISNLSPGVKYFYRAVIDGAVTDRIHSFKTFSAEKNYNFSFGFGSCQQ
                     GYGATSPDIFPVIAEDSLRFFIQTGDWTYPDFSDLGRGYNQSMDKLEESYRERYDYSY
                     PFNSGVLSMMPVSYLYDDHDYAGNDADGTNAYKLNSVKAYSMFFPHYELDNPDNGIWQ
                     KFRFGDVEFFLPDLRTQRSANADAIDAEGKFNPPAGHSILNGFTIDGEDQKTWLENSL
                     KNSDAKWKVIISTVIFNPRYSEALKNEQLGIMFPKVQKGIIDKWAGYPEDTEFLIDII
                     KNNDIKNVIMVSGDSHSSYIDDGANSVLPEISSSNLDTKNSKLGLLTQLAGYNIWNQG
                     SYNDDGHAYGRVSFYFGDEEYALLEVIDQTGKVVLSYRLDKK"
     gene            12671..13333
                     /locus_tag="PLX80_07385"
                     /pseudo
     CDS             12671..13333
                     /locus_tag="PLX80_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            13489..14442
                     /locus_tag="PLX80_07390"
     CDS             13489..14442
                     /locus_tag="PLX80_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20648.1"
                     /translation="MIRLKQKYGLLIIGIVLGIAYGLFTRLVFGQKATLATVTYLFIV
                     PTILGIIPLIFADIEKLKSYRNIIFIPLLTVSTFFLTMFLIGLEDFLCLFILAAPFFI
                     LGTIGAFIYRLVQINRQKNKGKLLTIILIPFLFAPVEEYLKSPSVTFEIESEVIISAA
                     PEMIWNNIVEVRTIRENEYSSGFFNDIGIPRPISANVDRKEIGGQRTGNFEGGLKFFE
                     TITKYEPDKIISFDIRIDPKSVRPNVFDQHVLSGNYFTFVNATYELTPLSNGQVKLKL
                     LSNYQLTSTINFYGKFWGDLILRDFQDRLLEVIEKRCGTKN"
     gene            14698..>15267
                     /locus_tag="PLX80_07395"
     CDS             14698..>15267
                     /locus_tag="PLX80_07395"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQY20649.1"
                     /translation="MNASFNIILLEFDWKSLLFGENDSAFLYVVFIRTAIMFLIILIG
                     LRMLGKRSVNQLSVFELGVIIGLGSAAGDPMFYESVGILTCILVFAVVVALYHLFRFL
                     VNNNQKLRFVLEGKPVVVIKDGEMCIDQIKKETSVQDVFLLLRLKSVSQLGQVHRAIL
                     EEDGQMSVFFFDDEEVKYGLPIIPEYIEHP"
BASE COUNT         5048 a         2567 c         2628 g         5024 t
ORIGIN      
        1 ttttgaaatt cttgggatct gaatatatgc tgcgtcagga gaaatatcag gatctaagcc
       61 gcttccggaa gatgttacca gttctgatgg aatttctttt ttattcacat ccggattatg
      121 taccataaac gtatctattc ttgcctgaac ttcagcaaga taatccgggt ttgttggacc
      181 tttgtttgaa ccaccggatg aagcagcgtt atatccgact gctgaaggtc ttccgttaaa
      241 atatttatca tctgaaaaat tctgaccaat gttttcaaac ccggttatct ttccgtttat
      301 ataaaccggc gaaccttctg atttttcagg tataagattt ccgattgcaa aaatgaagag
      361 tgggtaagca aatccaaaaa ttcctattgc aaaaattgtg agaattattg attgttttaa
      421 attactgaac atgactgata ttttatttta attttttaaa gcttaagaat attacaaaaa
      481 ctatttattc gttttgaaga tcttatatgt tttttatact atatgcagaa ccgtcaatac
      541 aacatcaatt aatttaattg cgataaacgg gacgataatt cctcccaatc catattttaa
      601 taaatttcct gccagcagtt tctctgctcc gacaggtttg taaaccactc ctcttaaagc
      661 aagcggaaca agcattggaa ttattattgc gttaaaaatt acagcagaaa gaatagcaga
      721 ctgtggactt gtaagattca ttatattcag tgcctgaagc tgaggaatag aagctatgaa
      781 taatgccgga actattgcaa aatattttgc cacatcattt gcaattgaaa atgttgtgat
      841 cgcgcctctt gtgatcaaaa gctgttttcc aatttcaact acttcaataa gcttggtcgg
      901 atcattatcc agatcaacca tatttcccgc ttcttttgca gcctgtgtcc cggaattcat
      961 tgacactcca acatctgcct gtgcaagcgc cggtgcatca tttgtaccgt cacccatcat
     1021 agctaccagt tttcctgatc tctgctcttc tataatataa ttcattttat cttcgggctt
     1081 tgcctccgca ataaaatcat ccacaccggc aatttttgct atgaactttg ctgtgagagg
     1141 attatctccg gttaccataa cagtcttcac tcccatcttc ctcaatctat gaaatctttc
     1201 ctgtattcca gtttttataa tatcctcaag ctggatcaca ccggcgggtt taccatttac
     1261 tgtaactgca agcggcgttc caccatcttc agcaattttt ttaactacgg aaatcatttt
     1321 ttcaggaaca atttcatttt tactttcgat cagatttttg atgacatcaa atgcgccttt
     1381 tctgacaata gtaccgtctt tcagatctat accgctcatt ctggtctgcg ctgtgaattt
     1441 aaaaaaatga tcatccctgt gttctatgtt attaatttcc actcccgatt tttttgcaag
     1501 ttcaatgatt gattttcctt cgggagtttc atcagcaagt gagctcaatg cacaatattt
     1561 tataaaaaca ttttcttcca ttccgtttac aataaaaaag ttagtggctt ttctgttacc
     1621 aattgtaatt gttcctgtct tgtccagcag aagtgtatca atatctccgg cagtttctac
     1681 tgcctttccg gatttggcaa ttacatttgc tctcaaagct ctgtccattc ctgcaattcc
     1741 gatagctgaa agaagtccgc cgattgtagt gggaatgaga caaacgaata atgcaacaag
     1801 agaagctatt gcaattggtg tgtttgaaaa tcttccaaaa ggttcaaggg caacagttac
     1861 tattaaaaac atcatagtaa gtcctgacag taaaattatg agtgcaattt catttggagt
     1921 tttttgtctt gatgcgcctt caacgagtaa gatcatttta tcaagaaatg attctccttt
     1981 ttcgatagaa atcaggactt tgattttatc tgacaatact tttgtaccac ctgtcacacc
     2041 tgatttatcg ccgccggctt ctcttatcac cggagctgat tcaccggtta ttgctgattc
     2101 atcaatagtt gcaatacctt ctattatttc tccgtccgaa ggaattattt caccttcact
     2161 gactaaaatt atgtcacctt ttttaagttc tgatgacata attattttta tattaccgcc
     2221 attgatcagt aaatttgccg gtgtgtcctg tcttgttttc cttagtgaat ctgcctgcgc
     2281 tttccctctg gcttcggcga ttgactcggc aaaatttgca aacagcacag tgagaaacaa
     2341 tataagtgtg attaaaaaat tatataaaaa gctgccctgt gcagcgtccg gggtgaatgc
     2401 aagcataaca gtaattacga tcattactat tgttccgatc tcaacagtaa acattacagg
     2461 attcttcacc attactttcg gattgagttt aacaaatgat tctattaatg ctgccttcag
     2521 aagttctttc tgaaataatt tattcgctgc tttattatga tttgacatag actttaaaat
     2581 ttattttgcc attgaaaaaa attctgaaac aggaccgagt gctaaacccg gaaaaaacag
     2641 caaagcacct aatattacta ttgttgataa taatacaatt ccgaaaatgg gcgagtcgat
     2701 ctttaatgaa cctgccgatt cgggtgtgaa ttttttcgca gataataatc ctgcaattgc
     2761 aacaggacca atgatcggaa tgaatcttcc caacagaatt acaataccgg ttgaaatatt
     2821 ccagaacgga gtattgtcaa tcagaccttc aaaaccggag ccgttatttg cagcggcaga
     2881 agaaaactca taaaacattt cagagaatcc atgataggaa ggattattaa gaaaattatc
     2941 ctgaatcggt gtaattaacc aaatgtgtgc ggctattgct gtacctgcaa gaattaataa
     3001 aggatgcatc agtgctacta taatagcaat cttaatttct tttccctcaa tttttttacc
     3061 gagaaactcg ggagtcctcc caaccattaa tcccgaaata aaaacagcaa gtattatgaa
     3121 tacaaattta tttaaaaagc ctacaccaac tcctccaaag aatgcattaa gctgcattcc
     3181 aagcatcagg atcattccgg taattggagt aaaactgtca tgcatagaat ttactgaacc
     3241 attagatgta actgtcgtgc tgactcccca cattgctgaa cttgcagagc caaaccggac
     3301 ttccttgcct tccatatttc caagcggttg tgaaattccc atcttgtcaa tttcaggatt
     3361 gccgttcatt tcaaaatata ttgccggaat taaaaaacaa acatatcctg taagcatgac
     3421 tccaaagatc atataggaaa gttttttccg gttcagatag tatcccaacg caattatcat
     3481 tgccaccgga ataaacagga ttgataccag ttcaattata tttgtaaaaa aattcggatt
     3541 ctcaaaagga tgtgcagagt tagttccgaa aaaacctcct ccgtttgttc ccagttgttt
     3601 tataccaacc attggcgcgg caggacctct tgctacactt acagtatcgc cctgcaatgt
     3661 gacaatattt tctgctcctt taaatgtcat tggaataccg ttgaacatta atattaatgc
     3721 aacaaccaca gagaccggca gcagaatcct tgtacaggat ttcagcataa gattataaaa
     3781 attgcctaag tttgttcctg atttatttgc aagtccctta aacattaatg cagcaacggc
     3841 aatgcctgta cctgcagaga caaattgaag aaaacacatt acaataagct gagaaagata
     3901 cgatacacca gtctctccgg agtaatgctg aagattggta ttagtcataa aactcacagc
     3961 cgtattgaat gcctgcgttg gttccatacc cggaatattg tcaggattcc agaaaagtaa
     4021 attctgaaat gaaagaacca gaattgccca gataaaccag atgaaattaa ttgtcagcat
     4081 agctttaata ttttgcttcc aatccatttc cttatctgga tctatgccgg aaaatttgaa
     4141 aattaaattt tccagtggtg ccagaaagtc gagaagtgtc ttttcatttt tgaaaacttt
     4201 tgaaatatat ttaccaaaag gaattcccag aataaaaata agcagaaaaa taaaaagtac
     4261 actgagtatt tcagtattca tatttgttta aaatttttca ggtttaatta atgtataagc
     4321 cagatacaca aagagaatta tacaaattat taatagtaca tacatttttg tttttttaga
     4381 ttttttcgag taaatcagcg aatttaaaaa ataaccggaa gaataatgct ccgagaagaa
     4441 acattaatat taaatcagtg atatccattt tgagatatta aatttcaaat atttatatgg
     4501 cactaattca ggaagtatgc caataaaata cttatgaaat ttgattaaaa gagagattta
     4561 tattccgtaa gaatttcaaa atgaaaaaag tttttcaaaa tggaaggtta ataagagatg
     4621 attggtgaag tttaatctac accgtattct ttcagttttc tgtaaagagt tactgcaccg
     4681 attccaagtt tttcggctgt gattgattta ttgtgtttgt tttcggaata aatttttaag
     4741 atatgctgct tttcaatttc tttgagtgaa tcggattgaa tctcgacagg aaatttatgc
     4801 tgatatattt cctcaggtaa atcctctgcc ataagttcgc ttcctttgca aagaatcact
     4861 gcacgttcaa ttagattctt cagttcacgg atattgccgg ggaatgaata ttcctttaaa
     4921 ccggaaataa aatcttcact tgctttggaa atattttttt tcagcttatc attataattt
     4981 ccaataaaat tatttattag taattcaaga tcatctttcc tttctcttaa tgccggaaga
     5041 taaattgtaa aagtgtttaa cctgtaatat aaatcatttt taaaatttac tttttcaatt
     5101 tcagatttta aatccttatt agttgcagaa atcacacgca catccacatg agttgttttc
     5161 gtatctccga cttttgttaa tgaatttgtt tccagtactc tcagaaattt cgattgagtc
     5221 tctaagctca tgtcaccaat ttcatcaaga aaaatagttc ctccgtcagc tgcttcaaaa
     5281 tatccctttt tatcttttac cgcaccggtg aatgcacctt taacatatcc gaacaattcc
     5341 gattcctgaa gatcttttgg gatcgcactg cagttaattg caatgaatgg tttattcttt
     5401 ctgctgcttg attcatggat tgcctgtgcg aataattcct tgcctgttcc tgtttcaccg
     5461 agaagcagga ctgtagtatc agtatctgct actttcttgg caagctctat agcaccggaa
     5521 atagacttgg ctttacctgt aattgaatta aaactaaagt tcctgtctat tttattttgt
     5581 aattctttta attttatttt aagtattgct ttatcgtatg cctgctttac ttttaaaatt
     5641 atttcgtcct catctttatc cttttctata taatcaaaag ctccgtttct cactgcttct
     5701 aaaccatcac gaatatttgc aaaagctgtc atgacaatta cttcagtatc aggggatttc
     5761 tcttttattt cttttgataa ctgaatgcca ttataatccg gaagaattac atctattaat
     5821 gctacataaa ataaattatt ttctattgct gtaagcccct cttttgaagt ttcacatgaa
     5881 gtgacagaat atccctccag tgaaagaatt ctttcaagta gatttcttaa attcctttcg
     5941 tcatcaacta ttaataaatt tttatcaaaa gccattaatt ttttctttat atattattta
     6001 attttgttaa acagaattta catactttca atatcaatct tttcttatta aaagaaaaga
     6061 tcctatatat ttattgttca taacaaattt tattccatcc aaatttaatc aagtccatat
     6121 taatatgtaa tgtaagtttg aaactcttaa attcattttg aaaattaatt cattaaattt
     6181 atttattaga tttgattgat tacggcaaaa tttattattc cgaaaaataa tacataggct
     6241 ttacaggaag tgcgcaatgg gttttcagca ttgaaaatac tattcggttt taatattttt
     6301 tccatttgaa aaatcatgag tatggaaaat tcattaaacc gatttttcag cagttatttt
     6361 aatttaactc cgcagtgaat acagaaaaca taatcagcag tgacattgga ctgatccatt
     6421 taattgcatc cgtacttgca ttaattaccg gatcattggt tttgtctttg acaaaaggta
     6481 cacggcttca caaaaaaatt ggatttactt atgtaacaag catgactata cttttaataa
     6541 ctgcttttat gatctaccgc ttgtttggaa cgtggggagt tttccattgg actgcactga
     6601 tcagctcctt gacgctttta tccgggatca ttccaattct gataaagcga cctgtgaaaa
     6661 attatatatt gtaccatttt agctttatgt actggagtgt aatcggactt tatggagcat
     6721 ttgcagccga gactcttgtg agattaccaa agattgtaat tgaaagtggt attccaaata
     6781 aagtttttta taatatgaca ggcattgcag ttgggttgac tatgggaatt ggtgcatact
     6841 tttttataaa actaaaatca aaatgggaga cacagtttgc acgaaaataa aacaaaggca
     6901 gacaaatgat ttgtaaaatc agcgggaaat tgcatactaa aagttttatg attttaaatt
     6961 tcaatttgct gatggaaatt gctgtgaaat ttaatccgcc cctacctaaa tcttaaagtg
     7021 atagaaatta ctttatacat tttaaaaata tcaatagaaa tattttttgg aaattaaaaa
     7081 atcctggaac ggtatttata ttttgaatta ttatttttca attcctaaaa tcttcgggtt
     7141 gagaaaaagt aatttgaaat taaagaagat gttttaattt agtggtatta cttaaaagag
     7201 caaatcagac attttttaat atttactttc ctcaaaaact aattaaccat tatattttat
     7261 aaattcattt ttgatttgta ttacttcaat taattaaaaa tttaaaaaaa aagtaatgtt
     7321 tttacatgtg ataatgatta gtgaaattgt tagtttgtta agttcaaaca tatccaaaac
     7381 attttcaaat ccggtttttt taatttctaa tttatgcctg aaaacattca aatcacagca
     7441 aacttaaaag aaaaatacaa agagatccta actgaagaag caataatttt tatttctgat
     7501 ctgcagaaga aatttaacag cagaagaaaa gaattattgg caaacagaga agtcattcaa
     7561 aatgaaattg actccggtaa aatgccggac ttccctgaag agacccgatc tatcagagag
     7621 agtgattgga aagttgcaga gatacctgat gacctgaaag accggagagt agagatcaca
     7681 ggtcctgtag acaggaagat gatcataaat gctttgaatt caggagcgaa tgtgttcatg
     7741 gcagattttg aagattcaaa ttctcccact tgggaaaatt tgatcgaagg acagatcaat
     7801 ttaagagatg ctgttaataa aaaaatttct ttcacaaacc ctgaaggaaa ggaatataaa
     7861 ctcaatgaag aagtagcggt tctaatggtt cgtccaagag gctggcatct tgaggagaaa
     7921 aatattttat tggataatga aaatttttct gcttcactat ttgattttgg tttgtatttt
     7981 tttcataatg caaaggatct catatcaaaa ggaacaggac catattttta tctgcctaaa
     8041 cttgagaact atcttgaagc gagattgtgg aatgatgtat ttaatttttc acaggatgag
     8101 ttaggtatcc ccagaggtac tatcagagca actgttctaa tagaaacaat tcttgcagca
     8161 tttgaaatgg atgaaattct atatgagtta aaagaccatt cagccggact aaactgcgga
     8221 agatgggatt atattttcag tttcataaaa aaattcagga attataatga ttatgttctg
     8281 cccgatagaa gcagggttac aatgaatgta cattttctga gatcttattc gcagcttgta
     8341 ataaagacct gccatagaag aggtactcac gctattggag gaatggctgc acagattcca
     8401 attaaaaatg atgaaaaagc aaatgaggaa gctatgtcaa aagtaaaatc ggataaagaa
     8461 cgggaagcag gtgacggtca cgacggtaca tgggttgccc atcccggact tatcactaca
     8521 gcaaaagaaa ttttcgacag attaatgccg gctcagaatc agatcgataa taaaagagaa
     8581 gatgtaaaaa tttctgcagc agatctgctg actgttccca aaggagaaat tacagaggaa
     8641 ggactccggc ataacataaa tgtatctata atatatcttg aagcctggct taatggaaat
     8701 ggttgtgttc ctatttataa tctgatggaa gatgctgcaa cagcagagat atccagagca
     8761 cagatctggc agtggatcca ttgcgaaaat ggaattctta atgatggtag aaaagtcact
     8821 tctgatcttt atcatgaatt agtaacagat gaacttaaaa aaataaaaaa cttagtcggg
     8881 gagaaaagat tttctgaagg taaatttgta aaatcaacag aattatttaa tgaactttct
     8941 acagacaggg aattttgtga attcttaact ctaactgcat ataaccatat taaataactt
     9001 taaataaata actatgaata aaactgacca gatcaatcag ataaaaaact cgtgggcttc
     9061 aggtgaaaga tggattggaa ttaaaagacc attttcagct gaagaagtat acaaactaag
     9121 aggctcagtt cagatagaac atacaatcgc aaaattagga gctgaaagac tatgggatct
     9181 tcttcataca gaaaaattta tcagagctct tggtgcatta acagggaatc aggcagtaca
     9241 gcaggttcag gcaggattaa aagcaattta tctcagcggc tggcaagttg ctgctgatgc
     9301 aaataatgcc ggacaaacat atccggatca gagtctttac cctgcggaca gtgttccatc
     9361 tgttgtcaaa agaattaata atgctttgat gagagcagat caaatttctc atatggaaga
     9421 taaaaatgat gtacattggt ttgcaccaat aattgctgat gctgaagcag gattcggagg
     9481 gaatcttaat gtatttgaac ttatgaaagg aatgattgaa gccggagccg gcggagttca
     9541 ttttgaagac cagctcagct ctgcaaagaa atgtggtcat ctgggcggaa aagttttagt
     9601 ctcaaccaga gaatttataa atacacttat agcagctaga cttgctgcag atataatggg
     9661 tgtatcaacc atactcatcg caagaacaga tgccaatagt gcgaatcttc ttttaaatga
     9721 tattgacgaa agagacagaa agttcataca tggtgaaaga acaatcgaag gtttttataa
     9781 aattaatggt ggacttgaat gtgctataga cagaggtttg tcttatgctc cttatgccga
     9841 tctgatctgg tgtgaaacag ctcatcctga tattgaagaa gcaaaaacat ttgcagatgc
     9901 aatacacgaa aaatttccgg gaaaaatgct agcttataac tgctcccctt ctttcaactg
     9961 gaaaaggaat ctgaatgata atcagatcgc agaatttcag gaaaaactcg gagaacttgg
    10021 ttataaattt cagttcatta cgcttgcagg atttcatgca cttaatacaa cgatgtttca
    10081 gctcgctctt gattataaaa gtgcaggtat gactgctttt acaaaacttc aggaacttga
    10141 atttgatctt gaaaaaactt caggttataa agctgttaaa catcaaagtt ttgttggaac
    10201 tggatatttt gatcaagtac aagtgtcaat atcgtccggt caatcctcaa ctattgcttt
    10261 agaaggatct actgaagctg aacaatttat aaaataattt ttataaatta aatcacctga
    10321 ctgctgtaaa accttagctg taaaagctat cggtgaatat atttcatcag ctatctcatt
    10381 taaacttact ccttttcctt cctgagatct gactgtaatg aacaccgctg cttttaaccc
    10441 atattcacag gcttttgata atatttatta ttttttgatt tttaattatt tatataatgt
    10501 attacaatta attgcattgg aagaaaatgt ttgatataac agatatcaac aattttccat
    10561 ttcaactata taatatcctt tttcaataat ttcagatttc cattttggcc ggatctcttc
    10621 tacatggcat cgtttgcatt catttgatgt caaagtctga tcatcctttc ctttaatttt
    10681 caaatattcc tttccgtagt tttgtattac tgactgatct tttacttctt caggagctat
    10741 aatgtcaaag tgtattattg tgacgtcttg cttggttaca tatgtatccc atactgtaac
    10801 tttcatttta tataattata ggtttgaaaa atctttactt tgtttaaaat ttaaatctaa
    10861 taaggacaaa attgtccgaa tcaaatttgt atttataact ttttaataac ttaacggaac
    10921 gaaatttaat atatcgatta atctaaataa ttatgaatga gtatgcatag atttttgtca
    10981 ctatacaatt gtttttacaa attcatactt ttattttata ataaatttag caattaataa
    11041 tttaatcaga agtaattact gatttaatta gtatcaatcc ttgatttaaa aactttaaaa
    11101 aatattatat gaccagactt ttgttaataa gctttattct tctttttact tctgtaaatt
    11161 gtaaaagcaa taactcaaca gacaggaaac ttccagataa taatttcaaa aatgatgagc
    11221 cgcgtattac tgatatcact ttaaaaaatt ccactcctca ggaaaattta attgaatcag
    11281 gtcccattgt cggcgcagtc actgaaaatt ctgcaacatt tctcgttaaa cctgtgaatt
    11341 cctcttcagt cagaatacaa ttgtcagaag attcttcatt cactgaacct ttctatacag
    11401 aagaaattaa cacagatcag aataatttca acttcacaaa aattcagata agtaatttat
    11461 ctcccggtgt aaaatatttt tacagagccg taatagacgg agcagtaact gacaggatac
    11521 attctttcaa aacattttct gcagagaaaa attataattt ttcatttggg ttcggttcat
    11581 gtcagcaggg atacggtgct acatcccctg acatattccc tgttatagct gaagactcac
    11641 tgagattttt tattcagaca ggcgactgga catatcctga ttttagtgat ctgggaagag
    11701 gatataatca aagtatggac aagcttgagg aatcataccg tgagagatat gattacagct
    11761 atccgtttaa cagtggagtg ctgagcatga tgccggtatc ttacctttat gatgatcatg
    11821 attatgcggg caatgatgct gacggaacaa atgcttataa attaaattca gttaaagctt
    11881 acagtatgtt ctttcctcat tacgaacttg ataatcctga taacggtatc tggcagaaat
    11941 ttcgatttgg cgatgtggaa tttttccttc cggatctgcg tacacagcgg agcgcaaatg
    12001 cagatgcaat agatgctgaa ggtaaattca atcccccagc gggacattcc attttaaacg
    12061 gatttactat tgacggagaa gatcagaaga cgtggcttga aaattctctc aaaaactccg
    12121 atgcgaaatg gaaggtcatt atctcaactg ttattttcaa tccgagatat tctgaagcac
    12181 taaaaaatga acaattggga ataatgtttc caaaagttca gaaaggcatt attgacaagt
    12241 gggccggata tcccgaagac actgaatttc ttattgatat aataaaaaat aatgacatca
    12301 aaaatgtgat aatggtcagc ggtgattcgc attcatctta catcgatgac ggagcaaatt
    12361 ctgtgttacc ggagatcagc agttccaatc tcgacacaaa aaattcaaaa ctcggattgc
    12421 ttacacagct cgccggctat aatatctgga atcagggaag ctataatgat gacggacatg
    12481 cttacggaag agttagtttt tatttcggag atgaagaata tgcattgctt gaagtaattg
    12541 accagacagg aaaagttgtt ttgagttaca gacttgataa gaaatagtga aggatattta
    12601 ctgaagcatc agaaataaat ttactttata acataattta aaagctatga ataaaatcaa
    12661 cgagtcagac aaattaattg atttaagcca tacaattgaa aacggacttg taacttataa
    12721 aggattgcct gctccgatta tctgcgatta tctgagcaga gaaaattcaa aaaagtttta
    12781 cgatgaagga acagaatttc agattggtaa aattgaaatg gtgtcaaata caggtactta
    12841 cattgattgt ccgtttcaca gatttgaaaa cggtaaagat ctatcagagg taggattgga
    12901 atgttttgtt gatttggatg caattgtcat aagcattcca tttattgaaa ccttagaaat
    12961 tactgaaggg catttgaaaa attatgaggt taatgacagg gcagttttaa ttcataccgg
    13021 ctgggacagg cattggaata ctgacctgta ttatgaaaac catccgtatt tgacagaaga
    13081 gtctgcgaaa tatttaagag attcaggtgc cagattagtg ggaattgact ctcataatat
    13141 tgacagtaca aacggcagga ccagacctgt acacacgaca cttttaggag cagaaatttt
    13201 aattgtagaa catctttgca atttaaattc attaccaaaa gacggtttta catttactgc
    13261 agttccacca aaattcaaag gtgtaggtac atttccggta agagctatgg ctaaattatc
    13321 aggaaaaata taacagaaaa acagttaact tttatctaag actatgaaac tgaaattaag
    13381 agatttctta aaatatttgt ccaaatagaa gcataaaaat gttaatcaaa attggtgctg
    13441 atttgccccg ccagcataaa gctctgaaac aaattatgcc ataacagaat gatacggtta
    13501 aaacagaaat acggactttt aataatcgga attgtcctag gtatcgctta cggacttttt
    13561 acaagattag tattcgggca aaaggcaact ttggctacag taacatattt atttattgtt
    13621 ccaaccattt taggaattat tccattaatt tttgcagaca ttgagaagct taaatcttat
    13681 cgaaatataa ttttcattcc cttgctgaca gtttcgactt tttttctgac aatgttttta
    13741 atagggcttg aagattttct ttgtttattc attcttgccg cgccattttt tatacttggt
    13801 acaatcggag catttattta cagactggtt caaattaaca ggcaaaaaaa caaaggtaaa
    13861 ttattaacca ttattctcat tccatttttg tttgctccgg ttgaagaata tctcaaaagt
    13921 ccttcagtga cttttgagat tgaaagtgaa gtaattattt cagctgcacc ggaaatgatt
    13981 tggaataata ttgtagaagt gagaacaatc cgggaaaatg aatacagttc cggtttcttt
    14041 aatgatattg gtattcccag accaattagt gcaaatgttg acaggaaaga aatcggcgga
    14101 caacgaacag gaaactttga gggaggatta aaattttttg aaacgataac taagtatgaa
    14161 ccggacaaaa tcatttcctt cgatataaga attgacccaa agtctgtaag accaaatgtt
    14221 tttgaccagc atgttttaag tggaaactac ttcacgtttg taaatgcaac atacgaactt
    14281 acgccacttt caaacggaca agtaaagctg aagcttttat caaattatca attgacttca
    14341 accattaact tctacggtaa gttttggggt gacctcattt taagagactt ccaggacaga
    14401 cttttagaag taatagaaaa gcgatgcgga acgaaaaatt aaaagatagt tagttttatt
    14461 taaagcaata aactccatac ataaagattt tttagtctaa tatttccatt tatgaaatgt
    14521 tttggacgct attggatgac acttaattcc cattgaaaat tacttatagt tatactgttt
    14581 ttaatttttt aaaattatta tttttatttt aaaaaaggat taagatctgt taaattataa
    14641 attaagttta catatatacc gatcatttta tttcttgaca ggatttatca caaaattatg
    14701 aatgcttctt tcaatatcat actattagaa ttcgactgga agtccctgtt atttggagaa
    14761 aatgactcgg cttttcttta cgtggttttt atacgtactg cgatcatgtt cctgataata
    14821 ctgattgggt taaggatgct gggaaaacga agtgtgaacc agttatcagt ttttgaactg
    14881 ggagtaataa ttgggctcgg ctcggcagcc ggagatccta tgttttatga gtcagtgggt
    14941 attttaacct gtatacttgt atttgcagtg gtggtagcat tataccatct gtttagattt
    15001 cttgttaaca ataatcaaaa actaagattc gtgctggaag gtaagcctgt tgtagtaata
    15061 aaagatggtg aaatgtgtat tgatcagatt aaaaaagaaa caagtgtgca ggatgtattt
    15121 ttgctgctga gattaaaatc agtatcacaa ctgggacagg ttcacagagc tatactggag
    15181 gaggacggtc agatgagtgt attctttttt gatgatgaag aagtaaaata tggcttgccg
    15241 ataatacctg agtatattga acatcca
//
LOCUS       DAORVQ010000071        15237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1721_length_15237_cov_5.778685, whole genome shotgun sequence.
ACCESSION   DAORVQ010000071 DAORVQ010000000
VERSION     DAORVQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_15237_cov_5.778685"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PLX80_07400"
     CDS             <1..1284
                     /locus_tag="PLX80_07400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20650.1"
                     /translation="KEYLNKVLIISYYFPPMGMGGVQRTLKFAKYLKDYGWDPVVLTD
                     SPKKYFAVDESLLKEAIDCGIKIERTGEDIKNVKDIIIKNPNERLRKVRSRTGQFFLI
                     PDNKKYWRNKAFKKAVDVWEKYGGFDVIFATAPPYTDFLVGQDVKKKFKVPLVIDYRD
                     AWVDSPVLNYYATPFHKMSNMKLEKNVLKDANKIITTNRRVKELVISRYGNIEYNDVK
                     IIPHGFDTEDFENAAQGELPITNKMRITYSGSFYTRNPKYYLEAINKFFIKFPELKSE
                     VEFCFIGAMSKENTALIESYNLGKSINLLGYIEHIECVKYLLASDVLFFMISRGENDD
                     AAMPGKIGEYIGSRKNIIACIPEGVTKKMLEKYNAIRFAEENPEDLMKIFHEYYGLYK
                     NNQMPVANEEVVELYDRKTLTYELAKEFNLLVDIE"
     gene            1380..2669
                     /gene="purD"
                     /locus_tag="PLX80_07405"
     CDS             1380..2669
                     /gene="purD"
                     /locus_tag="PLX80_07405"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQY20651.1"
                     /translation="MKILIVGNGGRENALAYAVYNSDSFKNNSGKIFITKGNPGSVKY
                     AESIDVEPTDINSLSDFALRENIDLTIAGSETPLSIGITDLFQSNGLRIFGPSKAAAE
                     IESSKIFSKELMKNSGVPTAEFRSFDESNINTSEDYFKNCKYPVVIKADGLAAGKGVI
                     IAEDFSRAMETVKDFTGGNTFGSAGRKFIAEEFLNGEEVSVFAITDGTDYVLLPFSQD
                     HKKISEGETGKNTGGMGAVAPVKKFMSDEMIRKINSKIIKPVLMEMKNTGREFKGCLY
                     CGLMIVNDEPFVIEFNCRFGDPETQAVLPLIESDFLELMFASSDNKISEYDLKIKDEY
                     ACCVVLASKGYPDKFEKGNLISGMEISNDDCIVFQSGTAISSDGRSVLTNGGRVLSVV
                     GISQSSLSDAKEKAYRNAELIEFENKYYRKDIGFKQL"
     gene            2685..3701
                     /gene="galE"
                     /locus_tag="PLX80_07410"
     CDS             2685..3701
                     /gene="galE"
                     /locus_tag="PLX80_07410"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQY20652.1"
                     /translation="MKILVTGGTGYIGSHTVVELQNEGYEVLIIDNLSNSSADVVNNI
                     EKISGVKPYFHKIDLCDRKALEEFFADQKDITAVIHFAAFKAVGESVNDPLMYYHNNV
                     TGMVNLLQEIQKNSINKFVYSSSCTVYGEADKLPVTENSPVKKAESPYGFTKQIGEQM
                     LFDISKISELNSIVLRYFNPTGAHDTSLIGELPIGIPNNLVPYITQTAIGKREMLTVN
                     GNDYDTPDGTNIRDYIHVVDLAKAHVLALKRMEDKKAETQIEVFNLGTGNGNSVLEVI
                     NSFENVTGLKLNYKIGPRRGGDVVSIFSDTKKANEVLGWKAERDLDDMMLTSWNWQKR
                     IGKD"
     gene            3852..4802
                     /gene="meaB"
                     /locus_tag="PLX80_07415"
     CDS             3852..4802
                     /gene="meaB"
                     /locus_tag="PLX80_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQY20653.1"
                     /translation="MTDEFLNNLVDKIRASDKRSIAKALTIIENESEGNEKLLKGLYK
                     FTGKAHRIGITGPPGAGKSTVTNGLLKLMNEKGLKTGVIAVDPTSPFTGGALLGDRIR
                     MQDSGLLENVFIRSMATRGSLGGLCKNVVEACDVLDASGKDFIIIETVGVGQSELDIA
                     QTADTTLVILVPESGDSVQAMKAGLMEIADIFVLNKSDRDGADAVAATIKNIIHLKPP
                     TPDNWIIDVLKSTASKNIGIEELFEKILEHKSHLMKSGYLKTKRQQQLKKKITELLTN
                     KLTVSFWGEDKKKYLNDNLELISEKKTDPYTFVEGMLDKK"
     gene            4973..7111
                     /locus_tag="PLX80_07420"
     CDS             4973..7111
                     /locus_tag="PLX80_07420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY20654.1"
                     /translation="MSKPKVQNTGIMNKMRDKMPLIIIILIIAFLATIVFEWGMNYMG
                     VGGQNEAFGKINDNEISYQEFERIVQQQIEQQRQQDPNTDIDEAKISQIREQVWSSLV
                     NQSLTRQAIEEYGITVSDREILDWIYNRPESLPEQIKRNFMDSTGVFNAGFYQQALGM
                     KTKEATQFWGQVETYVRETLLSEKLQAIISEGAIVTEGDVLQKYKDDNIFANIDYILL
                     DYNTVTDSSLFAVTDEDMKKYYEENKLDFIQKESMKLKYVNFPDLATPEDSVNVMKEL
                     ERYKKDLETATVEDSSLIKLVNENSSIPFNGDFQKANSFKAGATEFLFNAKPGDISKV
                     LITEEGYQIIKLLDSKETEEIFTNATHILIKTDTDSAAAYQKALDLYNRVKGGEDIKE
                     LAFELSDDPSAKQNKGDLGWFAKGAMVGEFETAANNATVGDVVGPVYTQFGFHIIKVN
                     GREKKEFKIAQITKPVAPSGRSKQLNKKKADEFYAELEKGKNIDTLAKELKLQIAESQ
                     DINRDGQIPLSSANKKVMNFGFDGKVGEYTEPVKILTGYAIYKITDKKPEGYQNFDSV
                     KVTLIKPKVITEKKALVLTGIAKDIESKIAGGDIMSLKDQYSQYIFGHSDSVSVSKPE
                     STIGLDHGVYSKIFKMNPGEISQPLKGQKGVIIVKLNSITEFNEQDYVLKAPEIRNTM
                     LASKKQQMVTDWLTKMQAEAKIVDNRDKYY"
     gene            7198..7932
                     /locus_tag="PLX80_07425"
     CDS             7198..7932
                     /locus_tag="PLX80_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20655.1"
                     /translation="MKLILKIFPLFILFISGISYSQSESEITLGSGFSIKTSLNYVST
                     ATIQLNAFSESVFERNKLEYVTGGYGYGILIRKKLFRDEISFGLSAEFVSIVDDELTQ
                     TFSSDSVTIRARVTEKLTMVPIELMGYFNIPEFAEDLQIYFGGGVGVYFGNRQRSLLN
                     IESKTLSKKPGFSLVVTSAMEYFFSRNISGVFEVRFRQGEYQVKSEFLVPQFTINGIS
                     YELEQTLNSKVFVDGLNISLGVAYNF"
     gene            7951..9606
                     /locus_tag="PLX80_07430"
     CDS             7951..9606
                     /locus_tag="PLX80_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY20656.1"
                     /translation="MIKLLVVFLISSSVFIFSGNNVSSELTGDYISRISVKKIFLKTK
                     KELPVSEATGEISLSSGISSLDKKLESYKVEKVKRVFKLNNGRKDLYEKFEMNRIYVL
                     FLSEENKFDIESIVSDLNEEESVEYCEPVSSGYAAGVKELQTKNIFSDSNPENNPNDE
                     MFYKQWYLQNNGSVDPTSGGLPKLGADIKIVYAWDIEEGSDEVVVAILDSGIKDDHPD
                     LKDRIWINSGEIPNNGIDDDYNGYVDDIKGWDFAYDDKRPEDGFGHGTNIATVIGAST
                     NNQIGFAGVDKKCRIMNCKNLNTYNSGEYDWWSESIKYAVDNGADIINMSEGGDDYSK
                     VLKTAIEYAIESGVLVVSAMMNKGDNRNYYPAGFDGVMAVGATDTDDRRCSRFSWGGG
                     SCWGDNISVVAPGNKIYGLDYENIDNYEVFWSGTSQSTAIVSAISSLLLAQNISRSSD
                     DLKKIIKFTSRDLVGDPREDTSGWDQYYGFGRVDGFAALTYESSADPEKLEEITKEKK
                     RKEIESVEENNKGNFNKDKATPENKDKEDVKSKSEYREPSKPK"
     gene            9646..10446
                     /locus_tag="PLX80_07435"
     CDS             9646..10446
                     /locus_tag="PLX80_07435"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQY20657.1"
                     /translation="MTSEFSEEIFLNSGLKGKIEKVIFFKELDSTNKYAKEYLKENPE
                     ADNTVILTSHQTDGTGRFNRKWFSSPGENLTFTLIKKFKLDVDEVYKVNFYSSYILFI
                     TLKEIFENEVDMNFILKWPNDLLLNNKKVSGFLFEVKDINIKEKTFIIGCGVNVNQTV
                     FDNSIKEKTTSLKLNSEKEVSPEDILNSYLKNFFGNISISFDSEELIKKWRSSSILSG
                     NIVTFKKYNDGISESATFEGIGNDGALCLRDKEGNIKKYFSGEITLSV"
     gene            10616..13438
                     /locus_tag="PLX80_07440"
     CDS             10616..13438
                     /locus_tag="PLX80_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20658.1"
                     /translation="MTKLFSLLSALLIFIAMNTQAQVSNYTFSQTSGTYSEFAGGTLV
                     AEAVNTATSNAGSLDDVNYSGQVIPFSFIFNGNSYTSYNLNSNAFISFGVTLPAGNTY
                     TPISGTVAYSGAVSLMGRDLQGIFSSTADRTLGSPVLTNVTSFSGVIAGKFITGTGIP
                     GTAAAPTVTIVSFDIGAGTITMSDNASSTGTAGAFAVASGTIRSMTTGAIGSRVHTIQ
                     YKNFKRFAGSNENMNLQIKLYETTNVIEYVYGLNTASSATSAAPQVGLRGAANSDFIN
                     RTGTDWSTSAAGGVNTATMTYNVTSLPASGLTYKWTPAPPPANDVGVTAINTPTGLYF
                     VGNPAVFPSVKIRNFGTSNQTTAFYTVYKITGPVAYADSTADTLASGYEKSITMPSSF
                     NFSTAGVYNVTAYTNLSTDTKRINDTLKSSFSVSNQSPNFGADSGYFYANSLATNQPS
                     YPKWGWKDTSGSRSIVLNGVIQPGMTNVGLLDDGYFKFKLSDILTQFGINPTNKKIKL
                     NGNEFDSLFVNTNGVIGLTEQFGTYSINDFNIDGAQVPHNAILAFWHDANFGTLTGGS
                     NRLSYKAVGNQVIITYDRAVSFAPTTDWVTFQVVLEVVSTGGSNSNWRTTFADTTNQN
                     TSSSFLANYNAQVAAVTPAATVFRNYMMGWTNTGPTAYSGYVSSGNPFAAVPITQVND
                     NSLLFFNGKGLAVEFGPNQNNLNKHDVVLVGVSISLEGLQSNNRVKDTVTVLLYDASS
                     SPNILIQSTKVYLDSVSSGGLKYGRKYIDFTALKRGAPVRIAVKHRNSITVYSSGTIS
                     TVSDTLKYDFTTSTSQTFGGNSVVINGKASSYTGDVNGDGVVDVSDVSAIDNDANNFI
                     SGPYVITDLNWDLVVDLTDAGLADNNANNFVSEIKPPGILSAYELPVFNINYKESQEI
                     RIIADPNAVPKIDINAAEDLNIKKED"
     gene            13625..14827
                     /locus_tag="PLX80_07445"
     CDS             13625..14827
                     /locus_tag="PLX80_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20659.1"
                     /translation="MKKTFKLFSVLFALVLFTNVSFSQIPQYTLEVKNSDILDPNTMV
                     FDLIFTHTNADLYEVAGWQFFFWVPATLGTFPVGFGGNASFVYDSTGGDAVSDLPPGF
                     RPRNANAVASSSNSVPGYELRLATNSVPGSGNGIIIPQGVPTRIGRYLMKSTTPIDLN
                     ALITNPFIIRDSCESQLSLTRTKVNSYVGTTNTEITRCANHSVSDGGALPVELSSFTS
                     GVSRNNVTLNWSTAKEINNSGFDIERSKVGTSEWTKAGNITGNGTTNDSKNYSFTERV
                     ETGNYSYRLKQVDFNGNFEYFNLSSEITVGVPDAYKLSQNYPNPFNPSTKIDYDLPYD
                     GKVSILLYDISGREVANLVNEVKTAGYYTAVFNGANLASGMYFYRINATGTGNNNFVS
                     TKKMVLIK"
BASE COUNT         5394 a         2316 c         2957 g         4570 t
ORIGIN      
        1 aaagaatatt tgaataaagt tttaataata tcatactatt tcccgccaat gggaatgggc
       61 ggtgtacagc ggacattgaa atttgcaaag tatctaaaag attacggatg ggatcctgta
      121 gtattaactg acagtccgaa aaaatatttt gcagtggatg aaagtctttt aaaagaagct
      181 attgattgcg gcatcaagat tgaaagaacc ggtgaagaca taaaaaatgt aaaagatatt
      241 attattaaaa atccgaatga acgtctgaga aaagtccgca gcagaaccgg tcagtttttt
      301 ctgattcctg ataataaaaa atactggaga aataaagcat tcaagaaagc tgtagatgtc
      361 tgggaaaaat atggcggttt tgatgtgatc tttgctacag ctccgcctta tacagatttt
      421 cttgtcggac aggatgtgaa gaaaaaattc aaagtgccgt tggtaattga ttacagagat
      481 gcatgggtgg acagtccggt attgaattat tatgcaactc cttttcacaa gatgtcaaat
      541 atgaaacttg agaagaatgt tttaaaggat gcgaataaaa ttattacaac taacaggaga
      601 gtaaaagagc ttgtcatttc cagatatgga aatattgaat acaatgatgt aaagataatc
      661 ccgcatggat ttgatactga ggattttgaa aatgctgctc agggtgaatt gcccattacc
      721 aataaaatga ggatcactta ttcagggagt ttttatacaa ggaatccgaa gtattatctc
      781 gaagctataa ataaattttt tataaaattt cctgaactga aatcagaagt tgaattttgt
      841 tttattggtg ctatgtcaaa ggaaaatact gcattgatcg aaagttacaa tctggggaag
      901 tcaataaatt tacttggtta catcgaacat attgaatgtg taaaatatct gcttgcctcg
      961 gacgtacttt tctttatgat cagcagaggt gaaaatgatg acgcagcgat gcccggaaag
     1021 atcggagaat acattggcag cagaaaaaat ataattgcat gcattccgga aggtgtcaca
     1081 aaaaagatgc tcgagaaata taatgctata cgttttgctg aagaaaatcc ggaagaccta
     1141 atgaaaattt ttcatgaata ttatggactt tataaaaata atcagatgcc tgttgcaaat
     1201 gaagaggtgg tggaactgta cgacagaaaa acactgacct atgagctggc taaagaattc
     1261 aatctgttgg tagatataga gtaaagattt tttgcacatt tttcatgttt taaattttag
     1321 gatctgtcga aaattaagaa taaacttatc cccgtaatta taaacctgat ttaattcaat
     1381 tgaaaatttt aatagtcgga aacggaggca gggaaaatgc tcttgcatat gctgtttaca
     1441 attctgactc atttaaaaat aattcaggaa aaatatttat aacaaaagga aatccgggtt
     1501 ctgtgaaata tgcggagagt atagatgtgg aacctactga cattaattca ctttcagatt
     1561 ttgcattaag agaaaatatt gatctgacga ttgccggttc ggaaacacct ttatcaatag
     1621 gtatcacaga cctgtttcaa tccaatgggc tcaggatctt cggtccttca aaagctgcgg
     1681 ctgaaataga atcgagtaaa attttttcaa aagaattaat gaaaaattca ggagtaccga
     1741 ctgctgaatt cagatcattt gatgaaagta atattaatac ctctgaagat tattttaaaa
     1801 attgtaaata tcctgtagta ataaaagcgg acggacttgc tgccggtaaa ggcgttatca
     1861 ttgctgaaga tttttcaagg gcaatggaaa ctgtaaaaga ttttaccggt ggaaatacat
     1921 ttggtagtgc cggaagaaaa tttattgcag aggaatttct gaatggagaa gaagtatctg
     1981 tctttgcaat tactgacggg acagactatg ttctgcttcc tttttctcag gaccataaaa
     2041 aaatttccga aggagaaaca ggaaaaaata caggaggaat gggagctgta gcgccggtca
     2101 aaaaatttat gtctgatgaa atgatcagaa aaataaactc aaagatcatt aaacctgtat
     2161 taatggaaat gaaaaataca ggcagagaat ttaaaggttg tctttactgc ggacttatga
     2221 tagtgaatga tgaaccattt gtgatagaat tcaactgcag attcggtgac cctgaaacac
     2281 aggctgtact tcctttgata gaatcagatt ttcttgaact tatgtttgca tcgtcagaca
     2341 ataaaatttc tgaatatgat ctgaaaataa aagatgaata tgcctgctgc gtggtgcttg
     2401 catctaaagg gtatccggat aaatttgaaa aaggaaattt aatttcaggt atggaaattt
     2461 ctaatgacga ttgtattgta tttcagtcag gcactgcaat ttcatcagac ggcagatcag
     2521 tattgacaaa cggcggaaga gttttatcag tggtaggaat ttctcaaagt tctttatctg
     2581 atgcaaaaga aaaagcttac agaaatgctg aacttataga atttgaaaat aagtattaca
     2641 gaaaagatat cggctttaaa caattataaa atatttttaa aaatatgaaa attttagtaa
     2701 caggtggtac gggatatatt ggatcacata ctgtcgttga acttcagaac gaaggctatg
     2761 aagttttaat tatagataac ctatcaaatt catctgctga tgttgtaaat aacatcgaaa
     2821 aaatttcagg tgtgaaacct tattttcaca aaatagatct ttgtgacaga aaagctcttg
     2881 aagaattttt tgctgatcaa aaagatataa cagctgtaat tcattttgct gcattcaagg
     2941 cagttggtga atccgtaaat gatccactga tgtattatca caataatgtg acaggtatgg
     3001 taaatctttt acaggagatc caaaaaaata gtataaataa atttgtctat tcatcttcat
     3061 gcacagtata tggtgaagcg gacaaattac ctgttacaga aaattcacct gtcaaaaaag
     3121 ctgagtcacc atacggattt acaaaacaga ttggcgagca gatgttgttt gatatttcga
     3181 aaatttcaga attaaattct atagtattaa gatattttaa tccgaccggt gctcatgata
     3241 cttcacttat tggtgagcta ccaatcggta ttccaaataa tctagtacct tatatcactc
     3301 agactgcaat aggcaaaaga gaaatgctca ctgtcaacgg gaatgattat gatacgcctg
     3361 atggtacaaa cataagagat tatattcatg tagtagatct ggcgaaagca catgtacttg
     3421 cattgaaaag aatggaagat aaaaaagccg aaactcagat tgaggttttc aatcttggta
     3481 caggaaatgg caacagtgtg cttgaagtta ttaattcttt tgaaaatgta acaggattga
     3541 aattaaatta taagatcggt ccaagaagag gtggagatgt tgtaagtatt ttttccgaca
     3601 ctaaaaaagc aaatgaagtt ctggggtgga aagctgagag agacctggat gatatgatgc
     3661 tgacttcatg gaactggcag aaaagaatcg gcaaagatta atttttttaa taataaaatt
     3721 tacgcatttt aaaattatat gcggattgct tttgacagaa ctataacgaa tcggagtttc
     3781 gttttgaaat tacatttcca aacagggtat ggaaacgggc acaattccgg aatagaaatt
     3841 acaataagat tatgacagac gaattcctaa ataatttagt agataagatc agagcttcag
     3901 acaaaagatc tatagcgaaa gcactaacga tcattgaaaa tgaatcagaa ggcaatgaaa
     3961 aattattaaa aggtttgtat aagtttaccg gcaaagctca caggatcgga attacgggac
     4021 ctccaggcgc aggaaaatct acagtaacaa atggtctttt aaagctgatg aatgaaaagg
     4081 gattgaaaac aggcgtaata gctgtcgatc ctacaagccc gtttaccggc ggtgcattgc
     4141 ttggtgacag gatcagaatg caggactccg gacttctgga aaatgtattt atcagatcga
     4201 tggcaacgag aggaagtctc ggcggattat gtaaaaatgt agtggaagct tgtgatgtac
     4261 ttgatgcatc gggaaaagat tttataatta ttgaaactgt cggagtaggt caatcagaac
     4321 ttgatattgc tcagactgca gatactactt tggttatact tgttcctgag tcgggtgatt
     4381 ctgttcaggc aatgaaagca ggtctaatgg aaatagcaga catctttgta ttaaataaat
     4441 ctgacagaga tggagcagat gcagtcgcgg caacaattaa aaacatcatt catctgaaac
     4501 cacctacacc ggataactgg ataatagatg ttttaaaaag taccgccagt aagaatatag
     4561 gtatcgaaga gttgtttgaa aaaatacttg aacataaatc acaccttatg aagtcaggat
     4621 atttaaaaac aaaaagacag caacaactta agaaaaaaat tactgaacta cttacaaaca
     4681 agctgacagt aagcttttgg ggagaggaca agaaaaagta tcttaatgat aatcttgaac
     4741 tgatctcaga aaaaaagact gacccttaca cttttgtaga agggatgctg gataaaaagt
     4801 aatatttagt ctgaaattga ttaacattgt atggaatatt gataagtaaa acaaaagtta
     4861 tattcctgcc aatcgctatt ataaattcat tgcttttaca aagcaaaaaa attaatttta
     4921 ctgtttcaca aaaccatttt ttaattaata tattaaggag cttttttaat taatgtctaa
     4981 accgaaagtg cagaatacag gtatcatgaa caagatgcgg gacaagatgc cgttgatcat
     5041 tattattttg ataatagcat ttcttgctac cattgtgttt gaatggggaa tgaattatat
     5101 gggagtagga ggacagaatg aagcctttgg aaagatcaat gataatgaga tttcgtatca
     5161 ggaatttgag aggatagttc agcagcaaat tgagcagcag agacagcaag atccgaatac
     5221 tgacattgat gaagcaaaaa tttcgcagat aagagaacag gtctggagca gtcttgtaaa
     5281 ccagtctctc accagacagg caattgagga atatggaatt actgtatctg accgtgaaat
     5341 attagactgg atatacaacc gtcctgaatc attacctgag cagataaaaa gaaatttcat
     5401 ggattctaca ggtgtgttta atgcaggatt ttatcagcag gcacttggaa tgaaaacaaa
     5461 ggaagcaact cagttctggg gacaggtaga aacttatgtc agagagactt tactttcgga
     5521 aaaactccag gcaataattt cagaaggagc tatagtaact gaaggagacg tacttcagaa
     5581 atataaggac gataatatat ttgcaaatat agattacatt ttactagatt ataatactgt
     5641 aacggattca tctttatttg cagttacaga tgaagatatg aagaaatatt atgaagagaa
     5701 taaacttgat ttcattcaga aagaatcgat gaagttaaag tatgtaaatt tccctgatct
     5761 ggcaacacct gaagattctg taaatgtcat gaaagaactc gaaagataca aaaaggatct
     5821 ggagacagct acagttgaag attcatcact tataaaactg gtaaatgaaa attcttcaat
     5881 accattcaac ggtgattttc aaaaagcaaa cagctttaaa gctggtgcaa cagaattctt
     5941 atttaatgca aaacctggag acatttccaa agtgttgatc acagaagaag gatatcagat
     6001 cattaaactg ttggattcaa aagagactga agaaatattt acaaacgcaa ctcacattct
     6061 gatcaagaca gatactgatt ctgctgctgc atatcagaag gcattagacc tttataatag
     6121 agtcaaaggc ggtgaggata taaaagaact tgcatttgaa ttgtctgacg atccttccgc
     6181 aaagcagaac aaaggtgatc ttggatggtt tgcaaaagga gcgatggtag gggaatttga
     6241 aacggctgca aataatgcaa cagttggtga tgtagtcgga cctgtgtata ctcagtttgg
     6301 ttttcatatt ataaaagtaa atggcagaga gaaaaaagaa ttcaaaattg ctcagatcac
     6361 aaaaccggtc gcaccaagcg gtagaagcaa gcagcttaac aagaaaaaag cagatgaatt
     6421 ttatgctgaa cttgaaaaag gaaaaaatat agatactctt gcaaaagaat taaaacttca
     6481 gatcgcagag tctcaggata ttaacaggga cggacagatt ccattaagct cagcaaacaa
     6541 aaaagtaatg aatttcggtt ttgacggtaa agtaggtgaa tatactgagc cggtaaaaat
     6601 acttacagga tatgcaattt ataagatcac cgataaaaag ccggaagggt atcagaattt
     6661 tgattcagta aaagtaacct taattaagcc aaaagtaatt actgaaaaaa aggcattagt
     6721 attaactgga attgcaaagg atatcgaaag taaaattgca ggcggagata ttatgagttt
     6781 aaaagatcag tattcacaat atatttttgg acattcagat agtgtcagcg tttcaaaacc
     6841 tgaaagtaca atcggacttg accacggtgt ctacagtaaa atatttaaaa tgaatccggg
     6901 tgagatctcg caaccattaa agggacaaaa gggagttata attgtaaaat taaattctat
     6961 tacagaattc aatgaacagg attatgtttt gaaagctccg gaaatccgaa atactatgct
     7021 tgcatccaaa aaacaacaga tggtaactga ctggcttact aaaatgcagg cagaagcaaa
     7081 gatcgttgat aaccgggata aatattattg attttaatgg tcagaaaaaa atgaattaaa
     7141 ctttaattta aaaagtgtta tttaaaccca ctataactaa actagtgggt tttttttatg
     7201 aagttaattc taaaaatttt tcccctgttc attttattta tatcgggaat atcttattcc
     7261 caaagtgaat cagaaataac cctcggcagc ggattcagta ttaaaacatc tttgaattat
     7321 gtatctactg ctacaataca gctaaatgct ttttctgaaa gtgttttcga aagaaataag
     7381 ctcgaatatg ttacaggtgg ttacggctat ggtattctga tcagaaagaa attattcaga
     7441 gatgaaattt catttggact gtctgcagaa tttgtatcaa ttgtagatga tgaattaact
     7501 cagacattca gttcggattc tgtaaccatt cgggcaagag taacggaaaa gctgacaatg
     7561 gtacctattg aattaatggg ttattttaat attcccgaat ttgcagaaga cctgcagatc
     7621 tattttggag gcggggtcgg agtttatttt ggaaacaggc aaagatcttt gttaaatatt
     7681 gaatctaaaa ctctttcgaa aaaacccgga ttcagtctgg tagttacaag tgcaatggaa
     7741 tattttttca gcaggaacat atcaggtgtg ttcgaggtta gattccggca aggcgaatat
     7801 caggtcaaaa gtgaattcct cgttccgcag tttacaataa atggaatttc gtacgaactt
     7861 gaacagacac taaactctaa agtgttcgta gatggtttga atataagtct aggtgttgca
     7921 tataattttt aaaatatatt accctcattt atgataaaat tattagtagt ttttttaatt
     7981 tcttcttcgg tatttatatt ctccggaaat aacgttagtt ctgagttaac gggggattac
     8041 atttcccgca tatctgtcaa aaaaatattt ttaaagacaa aaaaagaatt accggtttca
     8101 gaagctacag gagagatcag tctcagttca ggaatttctt cacttgataa aaaactcgaa
     8161 agttataaag tcgaaaaagt aaaaagggta ttcaaactta ataacggaag aaaagatctt
     8221 tatgaaaaat ttgaaatgaa caggatatat gttctttttt taagtgaaga aaataaattt
     8281 gatattgaat caatagtttc agatctgaat gaagaagaga gtgtagaata ttgtgaaccg
     8341 gtttcatccg gctatgcagc aggtgtaaag gaattacaga ctaaaaatat tttttctgac
     8401 agcaatcctg aaaacaatcc taatgatgaa atgttttata agcagtggta tctgcagaac
     8461 aatggttcag tagatcctac aagtggtgga cttcccaaac ttggtgctga cataaaaatt
     8521 gtttacgcgt gggatataga ggaaggcagt gatgaagtag tcgttgctat tcttgattca
     8581 ggtataaaag atgatcatcc cgatcttaaa gacagaatct ggatcaacag cggagagatc
     8641 ccaaacaacg gaattgacga tgattacaat ggatatgttg atgatattaa aggctgggat
     8701 ttcgcatatg atgacaaacg tcctgaagac ggatttggac acggtactaa tattgcaact
     8761 gtgattggcg cgagtacaaa taaccaaatt ggttttgcag gtgtcgataa gaaatgcagg
     8821 ataatgaatt gtaaaaacct caacacttat aacagcggcg aatatgactg gtggtctgag
     8881 tcgattaaat atgctgtcga taacggagca gatattatca acatgagtga aggcggtgat
     8941 gattattcta aagtattaaa aacagctatc gaatatgcaa ttgaaagcgg agtattagtt
     9001 gtttcggcaa tgatgaataa aggtgataac agaaattatt atccggctgg atttgatggt
     9061 gtgatggctg taggtgcaac agatacagat gacagaagat gcagtagatt ctcatggggc
     9121 ggtggtagct gctggggtga taatatttct gtagttgcac cgggaaataa aatctacgga
     9181 ttagactatg agaatattga caactacgaa gttttctgga gcggaacttc acagtcaaca
     9241 gcaatagtaa gtgcaatttc ttcattacta ctggcacaga atatcagccg aagttcagat
     9301 gatttaaaaa aaataatcaa atttacatcc agagatctgg taggtgatcc gagagaagat
     9361 acatccggct gggatcagta ttacggattt ggcagagttg atggctttgc agctcttact
     9421 tatgaatctt cggcagatcc cgaaaaatta gaagagatca caaaagaaaa aaagagaaaa
     9481 gaaattgaaa gtgtagaaga aaataataaa ggaaatttta ataaagataa agcaacacct
     9541 gaaaataaag acaaggaaga tgtaaaaagt aaaagtgaat acagagaacc ttcgaagcca
     9601 aaatagataa atacttttta aaaaattata attttttgat ctcaaatgac ttcagaattt
     9661 tccgaagaga tatttttaaa tagcggactt aaaggtaaaa ttgagaaagt tatatttttt
     9721 aaagaattag attcaacaaa taaatatgcc aaagaatatt taaaagaaaa tcccgaagca
     9781 gataatacag taattctcac ttcacatcag acagacggta ctggaagatt caacagaaag
     9841 tggttttctt cacccggaga aaatcttaca tttactctta ttaaaaaatt taaacttgat
     9901 gtagatgaag tttacaaagt aaatttctat tcatcttata ttctttttat tactttgaaa
     9961 gaaatttttg agaatgaagt agatatgaat tttatattga agtggccaaa tgatctgctt
    10021 ctgaataata aaaaagtatc ggggtttttg tttgaagtga aggacattaa tataaaagaa
    10081 aaaacattta tcatcggctg tggtgtaaat gtgaatcaaa ctgtttttga caattctata
    10141 aaggaaaaga ccacatctct aaaactcaat tctgaaaaag aagtatcgcc agaagatatt
    10201 ctcaattcgt atctgaaaaa tttttttggg aatatctcaa tatcatttga tagtgaagaa
    10261 cttatcaaaa aatggagatc ctcttccatt ctttcaggaa acatagttac atttaaaaaa
    10321 tacaatgacg gcatttcaga atctgcaact ttcgaaggca ttggaaatga tggcgcatta
    10381 tgtcttcgtg acaaagaagg taacataaaa aaatacttct caggcgaaat aaccctttct
    10441 gtctaaaatt caatcgtatt attatttttg ttagaatttt gttaggttaa tttttatttt
    10501 aataaaattg atctaagcct ctgaaccgat aaatgactat tgttttaata gactaattgt
    10561 tttattttta ggaaactttt ttaattaaca tattttaaca aactaaaaaa aaataatgac
    10621 aaaattattt tctttattat ctgcgttatt gattttcata gccatgaata ctcaggcgca
    10681 ggtatcgaat tacacttttt cacaaacttc cggaacttat tcagagtttg ccggaggaac
    10741 acttgttgca gaagcagtaa acactgcaac ttcaaatgcc ggatcacttg atgatgttaa
    10801 ctattcaggt caggtaatcc cattcagttt tattttcaat ggtaatagtt atacttctta
    10861 caatttaaat tcgaatgcat ttatatcgtt tggagttaca ttacctgcag gaaatacata
    10921 tacccctatc tcaggtactg ttgcttacag cggagctgtt tcattaatgg gaagagatct
    10981 tcagggaatt ttttcttcaa ccgctgacag aacacttggt agtccagtac ttaccaatgt
    11041 taccagcttt tccggtgtta ttgcaggaaa atttattact ggtaccggaa ttcccggaac
    11101 tgctgctgca ccaactgtaa ctatagttag ctttgatata ggtgcaggaa ctattacaat
    11161 gtctgataat gcttcttcta ccggaactgc aggagctttt gcagttgcat caggaactat
    11221 cagaagtatg actaccggtg ctatcggaag cagagttcac actattcaat ataagaattt
    11281 taaaagattt gcgggatcga atgaaaatat gaatctccag ataaagttat atgaaacaac
    11341 aaacgttatt gaatatgtat atggcttaaa tactgcttct tcagccacaa gtgcggcacc
    11401 tcaggttgga ctaagaggag cagcaaattc agattttatt aacagaacag gtacagactg
    11461 gtcaacttca gcagcaggcg gagttaatac agcaactatg acatataatg ttacttcact
    11521 tcctgcatct ggtttgacat ataagtggac acctgcaccg cctccggcaa atgatgttgg
    11581 tgttactgct atcaatactc caacaggtct ttattttgtt ggtaatcctg cagtattccc
    11641 ttctgttaaa ataaggaatt tcggaacatc aaatcagaca actgcatttt atacagttta
    11701 taaaataacc ggtcctgtcg cttatgctga cagtacagct gatacacttg catctggata
    11761 tgaaaaatct attacaatgc cttcttcatt taatttttct acagccggtg tttataatgt
    11821 tacagcatat accaatcttt caacagatac aaaaagaata aatgatacat taaaatcttc
    11881 ttttagtgtc tccaatcaat ctccaaattt tggtgctgac agcggatatt tttatgcaaa
    11941 tagtctggca acaaatcagc cttcttatcc gaaatgggga tggaaagata catccggaag
    12001 cagaagtatt gttctaaatg gagtaataca accgggaatg actaacgtcg gtcttcttga
    12061 tgatggttat tttaaattca aactctcaga tatattaaca caattcggta taaatccgac
    12121 caacaaaaaa ataaagctta atggaaatga attcgattct ctgtttgtaa atacaaacgg
    12181 tgtaatcgga ttgacagaac agtttggaac ttacagcatt aatgacttta atattgacgg
    12241 agctcaggtt ccacataatg caatactggc tttctggcat gatgcaaact ttggaacttt
    12301 aaccggaggt tcaaacaggt taagttataa agcagttgga aatcaggtca tcattactta
    12361 tgacagagca gtatcttttg caccaactac cgactgggta actttccagg tagtacttga
    12421 agtagtttca acaggcggat caaattcaaa ctggagaact acttttgcag atacaacaaa
    12481 tcagaataca agttcttcgt tcctggcaaa ttataatgct caggtagcag cagttacacc
    12541 tgctgcaaca gtattcagaa attacatgat gggctggaca aatacaggcc ctactgcgta
    12601 ttcaggatat gtatcatcag gaaatccatt tgctgctgta cctattacgc aggtaaatga
    12661 taacagtctg ttatttttta acggtaaagg acttgcagta gaatttggac caaatcagaa
    12721 taaccttaat aaacatgacg ttgtcttagt aggtgtaagt atttcacttg aaggcttgca
    12781 gagtaataac agagtaaaag atactgttac tgttttgctt tatgatgctt cttcaagtcc
    12841 gaatattcta attcagtcaa ccaaagtgta tcttgattct gtcagcagcg gtggtttaaa
    12901 atatggaaga aagtatattg attttactgc actgaaaaga ggagctccag taagaatagc
    12961 agttaaacac agaaattcta tcactgttta cagttcaggt acaatctcaa cagtttcaga
    13021 tactttaaaa tatgacttta cgacttctac atctcagaca ttcggaggca attccgttgt
    13081 tatcaacgga aaagcatctt catatacagg tgatgtaaat ggagacggag tagtcgatgt
    13141 atctgatgta tctgcaattg ataatgatgc aaataacttt ataagtggtc catatgtaat
    13201 cacagatcta aactgggatt tggtagttga ccttactgat gccgggttag cagataataa
    13261 tgctaataat tttgtatcag aaattaaacc tcctggaatc ttaagtgcat atgaacttcc
    13321 tgtatttaac atcaattata aagaatcaca ggagatcaga ataattgcag atccaaatgc
    13381 agttcctaaa attgatataa atgcggctga agatttaaat ataaagaaag aagactaatt
    13441 tgtaattcat ccttttcggg gcgggcaatg tcctgccccg catggggttt caaaaggaat
    13501 gctaaaagat ttctataatt gattttccgg agaaaattgt ttcggattta ctgaaagatt
    13561 tattttagta gaaaaagtaa tgaacaaacc acgtaatatt tttttattaa actaaacaaa
    13621 aaaaatgaaa aaaactttta aattgttttc agttttattt gctttggttc tgtttacaaa
    13681 tgtttcattc tcacaaatcc cccaatacac attagaagta aagaattcag atattttaga
    13741 tccaaatact atggtatttg atctgatatt tacccatact aatgctgact tatatgaagt
    13801 tgcaggttgg cagttctttt tctgggtacc tgctacttta ggaacttttc ctgtcggatt
    13861 tggaggaaat gcttcattcg tttatgactc tacaggtggt gacgctgttt cagatctacc
    13921 tcccggattc agaccaagaa atgcaaatgc tgttgcatca tccagtaatt ctgtcccggg
    13981 gtatgagtta agactggcaa ctaattctgt tcccggatca ggaaatggta ttataattcc
    14041 gcaaggtgta ccaacacgta tcggcagata tttaatgaaa tctacaactc caattgatct
    14101 gaatgcttta attactaatc cgtttattat cagagacagt tgtgaatctc aattgtcttt
    14161 aacccggaca aaggtcaatt cttatgttgg aactaccaac actgaaatta caagatgcgc
    14221 taatcacagc gtttcagacg gtggtgctct tcctgtcgag ctttccagtt ttacatccgg
    14281 agtttctaga aataatgtaa ctctgaactg gtcaacagcc aaagagataa acaacagcgg
    14341 ttttgatatt gaaagatcaa aagtcggaac ttctgaatgg acaaaagccg gaaatattac
    14401 aggtaacgga acgaccaatg attcaaaaaa ctattcattc acagaaagag tagaaacagg
    14461 aaattacagt tacagattga agcaggttga tttcaatggt aactttgaat attttaatct
    14521 atcaagtgag atcactgtag gtgtacctga tgcatacaaa ctttcacaaa actatccgaa
    14581 tccttttaat ccatctacta aaatagatta cgatcttcct tatgacggaa aggtcagtat
    14641 tttgctttat gacatttcgg gtagggaagt tgcaaactta gttaatgaag tgaagacagc
    14701 cggttattat acagcagtat tcaacggtgc taacttagct agcggaatgt atttctacag
    14761 aatcaatgca accggtacag gtaataataa ctttgtttca actaagaaaa tggtgcttat
    14821 caaataagtt aatagttaat agttaatagt tattttaatt tttaagattt aaaaaaggag
    14881 tctgtttaaa tggactcctt ttttttattt gtgattttac cctctcctaa gattcacgta
    14941 ctctttggac tcttcggttt aagcaccaat ttatcatctc ccattacttt agaatattga
    15001 atgacatcat aaattccttc tttaccaagt tcataaaatg caccacttga atgaatattg
    15061 taagaatatt actctaataa gtttccactt atctgatagc gggtttttat gaatggaatt
    15121 caatttttga ttgataaact tttcagtaag tatttactta atgacggcag atgtcataaa
    15181 tatttcatgc agcttacctt tcttttttac actttggctt acagcattat tcagtaa
//
LOCUS       DAORVQ010000072        15197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1732_length_15197_cov_5.983556, whole genome shotgun sequence.
ACCESSION   DAORVQ010000072 DAORVQ010000000
VERSION     DAORVQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 15197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15197
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_15197_cov_5.983556"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..266)
                     /locus_tag="PLX80_07450"
     CDS             complement(3..266)
                     /locus_tag="PLX80_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20660.1"
                     /translation="MEIKVIKKYLAIVFILFSLAFSFSNINFSQSQRHSIVYITGSND
                     FKDNVTDGFLKKNEEKKRHIYTVLSIKVFLLNSISEKIYFNSS"
     gene            384..1157
                     /locus_tag="PLX80_07455"
     CDS             384..1157
                     /locus_tag="PLX80_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQY20661.1"
                     /translation="MISGLIIGIIIGFIFTIPPLGPTYFQIIQQGLKNQTKNGLAIGA
                     GAGFIDFFYIMIAFGGVSLITSLLPDNIHQYFTENSETLKLILALGGSLFVIIYGVKI
                     ILAKQEFTHKLENEDQPGLKEKYDKVEKVFKKTESGIDKIFKTKVLEKHNSGITLSFI
                     AGVVSCLSSPTLPASWFAVVSFLKSYGLINSNFFTGFFLALGVFIGATGWFYILVNFV
                     SKNSVKINPRTLQKLNNYMGLFLVLIGISILVKLYFSGI"
     gene            1223..2707
                     /locus_tag="PLX80_07460"
     CDS             1223..2707
                     /locus_tag="PLX80_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HQY20662.1"
                     /translation="MDDLLKPVKNICTFCQMPIKNDSEATFCNSCSSPYHNDCWAENK
                     GCAVYGCGEKIRDDNEEVTIREAIINIEYLINRNQFSEAIFEGKQYLKLDRRNNELKN
                     LYNKAVALINNKINLMTSGDEAFGNKDYKAAEIYYKNVLKYTDETETNFVNTRIDVIR
                     EKIPEQRRRRLYTNILIIVIIIAILSALGYLGYYTFILKEDRDYSELVKSDKANDLRS
                     MEKMIGKYETFLRNYDDGKNKNRAMDRIKNYSYQISSIYYKDDWKLALKYYNKIKGSL
                     EPDDAKALYNNIYNIAYEEYMQKVSNGKKLNGNGKFSESMNELNNAKLILTVFPETPM
                     YKELNILEANISLLSKKIASVVKYNELEREIREQDKMLVNITSSGSPGSITRIISARV
                     TERRDDGIYLCREPDSRTIIAIKDKDENYTVGQAILVNTERTGDYKYRDYGKDIELAL
                     YEVIPRDAVPKSVDSEERQAILERINNLKDQKVKIDSLFKLSLL"
     gene            complement(2915..3061)
                     /locus_tag="PLX80_07465"
     CDS             complement(2915..3061)
                     /locus_tag="PLX80_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20663.1"
                     /translation="MTVTAKIASTTHGMCGPTKSADACSITGLSAGTYALIAARNDCS
                     AVLY"
     gene            complement(3058..3192)
                     /locus_tag="PLX80_07470"
     CDS             complement(3058..3192)
                     /locus_tag="PLX80_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20664.1"
                     /translation="MKRSMLFLAAVIFSAIALSNIQGIESKSDLNEIKNAILYSPDDE
                     "
     gene            3459..3541
                     /locus_tag="PLX80_07475"
     tRNA            3459..3541
                     /locus_tag="PLX80_07475"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3493..3495,aa:Tyr,seq:gta)
     gene            3556..3628
                     /locus_tag="PLX80_07480"
     tRNA            3556..3628
                     /locus_tag="PLX80_07480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3589..3591,aa:Gly,seq:tcc)
     gene            3657..3729
                     /locus_tag="PLX80_07485"
     tRNA            3657..3729
                     /locus_tag="PLX80_07485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3690..3692,aa:Thr,seq:ggt)
     gene            3896..4069
                     /gene="rpmG"
                     /locus_tag="PLX80_07490"
     CDS             3896..4069
                     /gene="rpmG"
                     /locus_tag="PLX80_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQY20665.1"
                     /translation="MAKEGSRVKIKLVSIEGPHKGKSVYVTYKNRVNTKERIELKKYC
                     KWTRQHILHRESK"
     gene            4098..4170
                     /locus_tag="PLX80_07495"
     tRNA            4098..4170
                     /locus_tag="PLX80_07495"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4131..4133,aa:Trp,seq:cca)
     gene            4212..4394
                     /gene="secE"
                     /locus_tag="PLX80_07500"
     CDS             4212..4394
                     /gene="secE"
                     /locus_tag="PLX80_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQY20666.1"
                     /translation="MKNKIITFFEDIFKEMKKVSWPKKEELIDSTKVVVITMLLSAAL
                     VYAIDKGISELLKVIL"
     gene            4409..5185
                     /gene="nusG"
                     /locus_tag="PLX80_07505"
     CDS             4409..5185
                     /gene="nusG"
                     /locus_tag="PLX80_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQY20667.1"
                     /translation="MTEEKNTEDPLEENINENTEEGTEEVSEEISEEISEDSGNDENV
                     SDDESDDVNNETDGNVAEDKAEPVIEEEEESEFMKWYAVRIISGHENKVKAYLDNEIR
                     NEKLHDKINNVLIPLEKVFEVKGGKKKVKFKNFLPGYILIEADLDEKIRDFISKTPSI
                     INMVGSKSVKGVKLDPIPLKRDEVKRIKSILNEEGDKEKLDIQLNVGDPVKVTSGPFN
                     NFSGNILEVNLEKFKVKVMVSIFGRKTPIELELTQVEKEK"
     gene            5204..5629
                     /gene="rplK"
                     /locus_tag="PLX80_07510"
     CDS             5204..5629
                     /gene="rplK"
                     /locus_tag="PLX80_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQY20668.1"
                     /translation="MAKKITGFIKLQIPAGAATMAPPVGPALGQRGVNGMEFCKQFNA
                     RTQDKKGYLIPVVITVFSDKSFTFITKTPPAAVLLIKALGIEKGSGVPNKTKVGKITQ
                     KQLREIAETKMPDLNVHDVEAAMSMIAGTARSMGLSVEK"
     gene            5702..6406
                     /gene="rplA"
                     /locus_tag="PLX80_07515"
     CDS             5702..6406
                     /gene="rplA"
                     /locus_tag="PLX80_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQY20669.1"
                     /translation="MKLTKKQKDNLKRVDIEKEYDLKTGITELKKTATAKFDESVDVA
                     VRLGVDPKHADQVVRGTVSLPHGTGKTVRVLVIAKPEKHDEAREAGADHVGYEDYVKK
                     IQDGWTDIDVIIAAPDTMAELGKIGKILGPRGLMPNPKSGTVTPNIGQAVKDVKAGKI
                     DFRVDKTGIVHSIVGKASFDENMLSDNITAFIDTIIKLKPAASKGTYVKSITLSTTMG
                     PGIKIDRNHSTAKTAA"
     gene            6502..7035
                     /gene="rplJ"
                     /locus_tag="PLX80_07520"
     CDS             6502..7035
                     /gene="rplJ"
                     /locus_tag="PLX80_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQY20670.1"
                     /translation="MKKAKKVESVSEIKGLLDNSEAIYFTDFAGLTVEEVNELRGDFF
                     KSGLKYKVLKNTLIVRALKETDKYSSHFEKLDEMLHGPTGIIFAKDNPVAPAKILKKF
                     FDKLERPKLKIAIVEDSAYDSKQLNTLAALPSKEELISSILSSLDSPASGIVGSVNAV
                     MRDLFSVIEEVGKKQAA"
     gene            7076..7447
                     /gene="rplL"
                     /locus_tag="PLX80_07525"
     CDS             7076..7447
                     /gene="rplL"
                     /locus_tag="PLX80_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQY20671.1"
                     /translation="MSVEEIAEKIGELTLTQASELKKILEDKFGVTAAAPMMMAGAPA
                     GGAAPAAEEQTEFTVILAEAGTQKINVIKAVKNATGLGLTEAKSLVESAPKPVKENIS
                     KADADKLKAELEAAGAKVEIK"
     gene            7617..11462
                     /gene="rpoB"
                     /locus_tag="PLX80_07530"
     CDS             7617..11462
                     /gene="rpoB"
                     /locus_tag="PLX80_07530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQY20672.1"
                     /translation="METTDIDLKLETVNSKNSRLTFAKTSITHDPPDLLNVQVQSYKD
                     FLQEDIPASKRKPQGLQKVFEDNFPISDSRETALLEFVDYYLEKPHYSVIECQEQGLT
                     YAVQVKARLRLSTKPNAAAKEYNYTIENDVYLGNLPYMTPRGSFIINGAERAIVSQLH
                     RSPGVVFGESTHANGTKLYSARVIPFRGSWVEFTTDINNLLYAYVDRKKKFYFSTLLK
                     ALGFNEHVDMLELFGLVENVKVNDKNYLDYVGRIVLENVLNKDTGDDIGINANSILSD
                     EQLVSIVGAKIKELRLLKEDPTDGEMVIYTTIINDEDETEELLEKLPADTSAKDKKAA
                     VALIESEAESARSYIEKLFFNTKKYDLGEVGRYKINYKLETNIDPHITILTQEDLVNI
                     INYIRDLVDGKKDVDDIDHLGNRRVRTVNEQLSAQFALGLSRMSRTIRDKMSIHDEEN
                     LTPSKLISARPITSALASFFGTSQLSQFMDQTNPLAEMTHKRRISALGPGGLSRERAG
                     FEVRDVHYTHYGRLCPIETPEGPNIGLISSLCIHSRINELGFIETPYRKVENGRVTDK
                     IEYLSAEIENYFKIAQANAPIDEKGKFVNDKIKGRFKGDFPLLSPDEVDYMDVATNQI
                     VSAAAALIPFLEHDDANRALMGSNMQRQAVPLLRPESPVVGTGFEEIIARDSRTLLTA
                     EADGVVEYVDGNRIVVKYNVKEDSDDSLLSFDDRKHVIYNLIKFLRTNQDTSINQRPI
                     VQEGQRIKKGDILADGSSTQNGELALGRNVLVAFMPWRGYNFEDAIVISEKLVMDDVY
                     TSIHIEEFSLEVRDTKRGEEELTKEIPNVSEEATKDLDENGIVRVGAEVKENDILIGK
                     VTPKGETEPTPEEKLLRAIFGDKAGDVKDASLKATPGLKGIVINNKLFSRKTRDTESK
                     RDEKRKIDKFEAERKKEIKELNNKIANKYDILLEGKTSKGMKDKKGNILVKSGAKFKS
                     GAFVNLIENEGYLLADFDYDSEWADYSRANTLVPDIYRNYTFKQNEIEERYKRLKDKV
                     KLGDELPTGVVKLAKVYVAKKRKLSVGDKMAGRHGNKGVVAKLVPMEDMPFLPDGTPV
                     DIVLNPLGVPSRMNLGQLYETALGWAAKKMGVKFATPIFDGATVAEIVDMLKLAGIDE
                     NSRTYLFDGMTGERFDQRVTCGYIYMLKLSHLVDDKIHARSIGPYSLITQQPLGGKAQ
                     FGGQRFGEMECWALQGYGAANILQEMLTYKSDDVTGRSKVYEAIIKGDNLPEPGVPES
                     FNVLLKELQGLCLDINLDE"
     gene            11552..>15197
                     /gene="rpoC"
                     /locus_tag="PLX80_07535"
     CDS             11552..>15197
                     /gene="rpoC"
                     /locus_tag="PLX80_07535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQY20673.1"
                     /translation="MAFKTEHKRKRFSKITINLASTDAIITKSFGEVLKPETINYRTF
                     KPDKDGLFCEKIFGPVRDWECHCGKYKGIRYKGIVCDRCGVEINLKNVRRERMGHITL
                     SVPVVHIWYFRSLPTKIGYVLGISSKELERIIYYETHVVINPGKSNYKRLQLITEDEY
                     NEVLDSMTEEEMNLDDDDPKKFYAGQGGEAVKELLRSVNTEEEIARLRAELKDDPSAL
                     KKMENIKRLRILEAFRIKPGGRPNKPEWMVLDVVPVIPPELRPLVPLEGGRFATSDLN
                     DLYRRVIIRNNRLKRLMDIKAPEVILRNEKRMLQEAVDALLDNSRRVTAVKAENRALK
                     SLSDILKGKQGRFRQNLLGKRVDYSGRTVIVVGPELKLHQCGLPKDMALELFKPFVIR
                     RLIDRDYVKTVKSAKKLIDKRTPEVWDILENVINGHPVLLNRAPTLHRLSIQAFQPIL
                     IEGKAITLHPFVCIAFNADFDGDQMAVHVPLSYEAQLEAQIMMLSSHNILSPQNGAPI
                     TIPNQELILGCYYLTKELPGDLGEGKIFSNSDEVMLAFDNKKVGLHARIKVRFRGEII
                     ETTVGRVIFNRCVPEKIPFINELLKKKKIIEMINMIYNRVGNLETSVFLDNLKEIGFK
                     YATMGGLSVNIDDIEVPATKDKIIEEAFLRVDRIEKSKAKGIISENERYNKVIDIWTH
                     VRDEVKRDLMRNLLSSKQGFNSLHMMIDSGARGSAEQVSQLAGMRGLMQKPQKSMTGQ
                     AGEIIENPIIANFKEGLSILEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVI
                     IIEVDCNTIRGVTTEALKEGEDIKESLAERILGRVAVHDVVNPLTKKVIIKGGELINE
                     ELADMVAETPITEVEIRSVLTCELKRGVCAKCYGRNLATGKMVEVGEAVGIVAAQSIG
                     EPGTQLTLRTFHIGGTASKIISQSQISTKFEGKVKFENIKIVEYKGGAGTEFVSLSRN
                     GLINIMDENGSQLARYTVPYGTHLKVKDKEIVPKNAILYEWDPYNSVIVAEDDGVINY
                     FEMDEGKQYDISKDEQTGFLQKIVIESRDKTKSPTIQILNPKNEKLMSSYLIPAKASL
                     LVDNGADVKAGTIMVKIPRESGKTRDITGGLPRVTELFEARSPNDPAKVAEINGTVEI
                     GKISRGSREVRIISKDGSKVIDYKIPIGKHILVRHGDIVKSGEPLTGGAYDPVDILKI
                     KGVAEVQEYLV"
BASE COUNT         5558 a         2319 c         3235 g         4085 t
ORIGIN      
        1 tgctacgatg aattaaaata aattttctcg gaaatcgaat ttaacaaaaa gactttaata
       61 gaaagcactg tgtatatatg tcttttcttt tcttcatttt ttttcagaaa gccatcagtg
      121 acattatctt taaaatcatt agatccggta atataaacaa ttgaatgtct ctgactttgt
      181 gaaaagttta tattggaaaa agaaaatgcg agtgagaata aaataaagac aatcgctaaa
      241 tattttttta taactttaat ttccattggt tcaaaatttt gtagataaaa ttacaaaatc
      301 cgctccaata attcaactta accataagtt tctattaaat tgaaaataac agtgtgttta
      361 actaatttaa aaaaaaatat tttttgattt ccggattaat tatcggtatt ataattggtt
      421 ttatttttac gatcccacca ctgggaccga catattttca gataatacag cagggactaa
      481 aaaaccagac caaaaacggc ctggcaatcg gggcaggagc cggattcatc gatttttttt
      541 atattatgat cgccttcggc ggagtatcac ttattacaag tctgctgcct gataatattc
      601 atcaatattt tacggaaaat tcagaaacat taaagctgat acttgcatta ggcggaagtc
      661 tgtttgtgat tatttatgga gtcaagataa ttcttgctaa acaggaattt acacacaaat
      721 tagaaaacga agatcaaccg ggtcttaaag aaaagtacga taaagttgaa aaagtattta
      781 agaaaacaga atcaggaatt gataaaattt ttaagacaaa agtactggag aaacataatt
      841 cagggatcac tttaagtttt atcgcaggag tagtttcctg tctctcatcg ccaacattgc
      901 ctgcatcatg gtttgcggtg gtcagctttc tgaaaagcta tggtctgata aattcaaatt
      961 tctttaccgg tttttttctt gcactgggag tttttattgg agctacggga tggttttata
     1021 tactagtgaa ttttgtttca aaaaattcag taaaaattaa tccccgtact ttgcaaaagc
     1081 tgaataatta tatgggattg tttcttgtat taatcggaat ttctatatta gtaaaattgt
     1141 atttcagcgg aatatgaatt ttgttatttt tcagatatta aaaattttat aacttaccgg
     1201 aaattttatc atttccaaaa aaatggacga tctcttaaaa cctgtaaaaa atatctgcac
     1261 tttttgtcag atgccgatca agaatgattc ggaagctacg ttctgtaact catgcagctc
     1321 tccttatcat aatgactgct gggcagagaa taaaggttgc gccgtttacg gatgcggtga
     1381 aaagatccgc gatgataatg aagaagtaac gataagggaa gcaattataa atattgaata
     1441 ccttattaac aggaatcagt tttctgaagc tatatttgaa ggtaagcagt atttgaaact
     1501 ggacagaaga aataatgaat taaagaattt atacaataaa gccgttgcgc tgataaataa
     1561 taagatcaat ctgatgacca gcggtgatga agcattcgga aacaaagatt ataaggctgc
     1621 agaaatttat tataagaatg ttttaaaata tactgatgag acggaaacta attttgtaaa
     1681 tacaagaatt gatgtgatca gggaaaaaat tcccgagcag agacgaagaa gactttatac
     1741 caacattctt attattgtaa ttattattgc catactttca gctttagggt atctcggata
     1801 ttatactttt attttaaaag aggacagaga ttattccgaa cttgtaaagt ctgacaaggc
     1861 aaatgatctc agatccatgg aaaaaatgat cggcaagtat gagacatttc tgcggaatta
     1921 tgatgacgga aaaaacaaga accgcgcaat ggacaggata aaaaattatt cttaccagat
     1981 ctccagtata tattataaag atgactggaa actcgcattg aaatattata ataaaataaa
     2041 aggaagtctg gagccggatg atgcaaaagc tttatataat aatatttaca atatcgcata
     2101 tgaagagtat atgcaaaaag tatcaaacgg taaaaagctg aatggaaatg gaaaattcag
     2161 cgagtctatg aatgaactga ataatgccaa acttatcctg actgtatttc ccgaaactcc
     2221 gatgtacaaa gaactgaata ttctcgaagc caacatctct ctactcagta aaaagatcgc
     2281 atctgtagta aagtataatg aacttgaaag agagataagg gaacaggata agatgcttgt
     2341 gaatattaca tcttccggaa gtcccggatc catcacacgc attatttctg caagagttac
     2401 tgaaagaagg gacgatggaa tttatctctg cagagagccg gattcaagga ctattattgc
     2461 aataaaagac aaagacgaaa actatacagt agggcaggca atacttgtaa atactgaaag
     2521 gacaggtgat tataaataca gggattatgg aaaagatatt gaactggctt tatatgaagt
     2581 gatcccaaga gatgcagttc cgaaatctgt ggattcggaa gagcggcagg cgatactgga
     2641 aaggatcaat aatctgaaag atcagaaggt gaaaatagat agtttgttta agcttagtct
     2701 tttgtagatt atatgcctgc ctcgaagact cgaaggctcg aaggcttaaa ggagaatact
     2761 atttatagta atttcaaacg cagccgacac aagcagaaat aagattcaca atgaaaacag
     2821 gttttacatt gtaacatcaa tcagcgatta ttatcagccg gtttttgttg aacttttttt
     2881 acataacttc agaaattctt ctaactcagt aaaattaata taaaaccgct gaacaatcgt
     2941 ttcttgccgc gattaaagca taagtcccgg cagataatcc tgtgatcgaa caagcatctg
     3001 cagattttgt cggaccacac ataccatgtg tagtcgaagc aatctttgct gtaacagtca
     3061 ttcatcatca ggtgaatata gaattgcatt ttttatttca ttcagatctg atttactttc
     3121 gataccctga atattggata aagcgattgc cgaaaatatg acggcagcta aaaaaagcat
     3181 tgagcgtttc atttctacta cttagtttaa atgttgctga aatgatcaaa ataattttta
     3241 aaaagttttt aagtttataa cgctcatcat aattataaat aaaaaaaaat caatttatat
     3301 tttaatggat attaatgtaa ttttatatat taacgtaata aatatgaatt caaaaaaagc
     3361 taatcggaca tcaaaatatg agttgattta aaatgttaaa tttgatattt ttaaagtcct
     3421 ttgctgtata ataattttac gttcaggaaa ttttaaatgg gtaggtagcg aagtggttaa
     3481 acgcaacaga ctgtaaatct gtcgactcag gtcttcggag gttcgaatcc ttccctaccc
     3541 acgaaggaaa aataagcggg agtaactcag ttggtagagt cacagccttc caagctgttg
     3601 gtcgcgagtt cgagtctcgt ctcccgctct ttgatacaga ggaaaaaaac tgataagctg
     3661 atgtagctca gttggtagag cacatccttg gtaaggatga ggtcaccggt tcaatcccgg
     3721 tcatcagctc tcgagcagaa agattcaatc ttcagattaa atttgaaatg agattgaaaa
     3781 gatcagaaaa aaaataaaag taaaataaaa aagttaaata acattcctga aaataaaact
     3841 ctgttgtaag ttagttactt ttaaaaaacc aataaatttt tagaaaaata atatcatggc
     3901 aaaagaagga tcaagagtca aaatcaagtt agtaagtatt gaaggacctc acaaaggaaa
     3961 atccgtttac gtcacataca aaaacagagt aaacacaaaa gagagaatcg agttaaaaaa
     4021 atattgcaaa tggaccaggc agcatattct acacagggaa tcaaaataaa ttgatacctt
     4081 caattaagtg accaactgcg ggagtggctc aattggtaga gtagcggtct ccaaaaccgt
     4141 tggttggggg ttcgagtccc tcctcccgtg caaatggatc tcaaaataat tttatagttt
     4201 acatataaaa aatgaaaaac aagataataa cttttttcga agatatcttt aaggaaatga
     4261 agaaagtttc atggcctaaa aaagaagaac taatcgattc tacaaaagta gttgtaatta
     4321 caatgctttt atcggctgca ttagtgtatg cgatagataa aggaatcagc gaactgctga
     4381 aagtgatatt ataataatac atatttaaat gactgaggaa aaaaacacag aagatccatt
     4441 ggaagagaac ataaatgaaa atactgaaga aggtacagaa gaagtttccg aagaaatttc
     4501 agaagaaatt tcagaagact ccggaaatga tgaaaacgtt tcggatgatg aatctgatga
     4561 tgttaataat gaaacagacg gaaatgtagc tgaagataaa gctgaacctg taattgaaga
     4621 agaggaagag tctgaattta tgaaatggta tgctgtaaga ataatttccg ggcatgaaaa
     4681 taaagtgaag gcatatttag ataatgagat cagaaatgag aaacttcatg ataagatcaa
     4741 taatgtatta attcctctcg agaaagtatt cgaagtcaaa ggcggaaaga agaaagttaa
     4801 attcaaaaat ttcttaccgg gctatatact tatagaagca gatcttgatg aaaagatccg
     4861 tgattttatt tccaaaaccc cgtctataat aaatatggtc ggttcaaaaa gtgtaaaagg
     4921 tgtcaagctg gatcctattc cattaaagcg tgatgaagta aaacggatca aatcgatatt
     4981 gaatgaagaa ggtgataagg aaaaattaga tatacaactg aatgtaggag atccggtaaa
     5041 agtaacaagc ggacctttta ataacttcag cggaaatata ctcgaagtaa atcttgaaaa
     5101 gtttaaagta aaagtaatgg taagtatttt cggaagaaag acacctatcg agcttgaact
     5161 tacacaggta gaaaaagaaa aataatatta atatttatat aaaatggcaa aaaaaataac
     5221 aggttttatt aagctacaga tccccgcagg tgcagctact atggctcctc ctgtcggacc
     5281 ggctcttggt cagagaggcg taaacggaat ggaattctgc aagcaattca atgcaaggac
     5341 acaggataaa aagggttacc ttattcccgt ggtcattact gtattttctg ataagtcttt
     5401 tacatttatt acaaagactc ctcctgctgc agtacttctg atcaaagcac ttggtatcga
     5461 aaaaggatcc ggtgtaccaa ataaaactaa ggtaggtaaa atcactcaga agcagttaag
     5521 agaaattgca gaaacaaaaa tgcctgatct taatgtgcat gatgtagaag cagcaatgag
     5581 tatgatcgcc ggaactgcaa gaagcatggg gctttcagta gagaaataaa ttgcatctgc
     5641 attttattaa aaaaagatat acaaagatac ttttaaattt tttaattttt caaatttcaa
     5701 aatgaaatta actaagaaac agaaagacaa cttaaaaaga gttgatatag aaaaagaata
     5761 tgatcttaag acaggaatta cagaacttaa gaaaactgca acagcaaaat ttgatgaatc
     5821 tgtagatgtg gcagtaagac tcggagtaga tccgaagcat gctgatcagg tagtaagagg
     5881 tacagtgtca cttcctcatg gaacaggtaa aactgtcaga gtacttgtga tagcaaaacc
     5941 tgaaaaacat gatgaggcaa gagaagccgg agcagatcac gtcggatatg aagattatgt
     6001 aaaaaagatt caggatggat ggacagatat tgatgttatc attgctgcgc ctgatactat
     6061 ggctgagctt ggtaaaattg gaaagatact cggaccaaga ggtcttatgc cgaatccaaa
     6121 aagcggaacg gtaactccga atatcgggca ggcagtcaaa gatgtaaaag ctggaaagat
     6181 cgattttaga gttgacaaga caggcattgt acattctata gtaggtaaag cttcatttga
     6241 tgagaatatg ctttcagata acattacagc atttatagat acaataataa aattaaaacc
     6301 ggctgcatca aaaggaacat atgtaaagag cattacttta tccactacta tgggacccgg
     6361 aattaaaatt gacagaaatc attctacagc aaaaactgct gcataacaat taagaattta
     6421 cgcactcaac aaaacatata ttttgaatac cagcttcagg tatacagaaa agatccaaat
     6481 tattacaaaa taagctaaat tatgaaaaaa gcaaagaaag tagagtcagt cagtgagatc
     6541 aagggtttac ttgataactc tgaggctatt tactttactg atttcgcagg attaacagtc
     6601 gaggaagtga atgaattacg aggcgatttc tttaaatccg gattaaagta taaggtatta
     6661 aaaaatactt taattgtccg cgcattaaaa gagactgaca agtattcaag tcattttgaa
     6721 aagctcgatg aaatgctgca tggtccgacg ggaattattt ttgcaaaaga taatcctgta
     6781 gcacctgcaa agatcctgaa gaaatttttt gacaaacttg aaagacctaa gctcaagatt
     6841 gcaatagtag aggattcagc atatgattca aaacagttaa acacgcttgc ggcacttcct
     6901 tcgaaagagg aattaatttc gtcaattctc agctcactgg attcaccggc atccggaata
     6961 gtcggatcgg taaatgcggt aatgagagat ctgttcagtg taatagaaga ggttggcaag
     7021 aaacaggctg cataaataaa ttttaaaaaa ttaaaccaaa cagaggtaaa ataaaatgtc
     7081 agtagaagaa attgcagaaa aaataggtga gttaacatta actcaggcat cagaattaaa
     7141 gaaaattctg gaagataaat tcggtgtaac agcagcagct ccaatgatga tggctggcgc
     7201 acctgcaggc ggagcagctc cggcagcaga agagcagact gaattcacag ttatacttgc
     7261 agaagcaggt acacaaaaga tcaacgtgat caaagcagta aagaacgcaa caggacttgg
     7321 acttaccgag gcaaaatcac ttgtagaaag cgctccgaaa cccgttaagg aaaacatttc
     7381 aaaagcagat gcagataagc taaaagctga actggaagct gccggtgcaa aagtcgaaat
     7441 taagtaattt tattttcggg cactattgta tcaaatatat caattgtaac aaaattattt
     7501 attgtatcat ttgtaacaat tgaatcaaat gtatcaaaaa aattaattta taaaacataa
     7561 tagtcccggc aaattcatgc cgggattatt tattaaaaaa ttccgggggt aaaaatttgg
     7621 aaactacgga tatagatctt aaattagaaa ctgtaaacag taagaatagc agacttacgt
     7681 ttgcaaaaac ttcgattaca catgatccgc ctgatctgtt aaatgttcag gtacagtctt
     7741 ataaagattt tcttcaggaa gatatacctg caagcaaaag aaaaccgcag ggtcttcaga
     7801 aagtctttga agataatttt cctatcagcg attccaggga aacggctttg ctggaatttg
     7861 tagattatta tttagaaaaa cctcattact cagtaataga atgtcaggag cagggattga
     7921 cttacgctgt tcaggtaaag gcaagactga gattatccac aaaaccgaat gccgctgcaa
     7981 aagagtataa ttacacaata gaaaatgatg tatatcttgg aaatctacct tatatgactc
     8041 caagaggaag cttcattatt aacggtgcgg agagggctat tgtaagtcag cttcacagat
     8101 caccgggcgt agtattcgga gagtcaacac atgcaaacgg aacgaaactt tattctgcaa
     8161 gggtaattcc tttcagaggt tcatgggtag aatttacgac tgatattaat aatctgcttt
     8221 atgcttatgt cgacagaaaa aagaaattct atttctcgac actgctgaaa gcgcttggat
     8281 ttaatgagca tgttgacatg cttgagctgt ttggactggt tgaaaatgta aaggtaaatg
     8341 ataagaatta tctggattat gtaggcagga tcgtacttga gaatgtattg aataaagata
     8401 ccggagatga tattggaata aatgcaaaca gcatactttc agatgaacag cttgtgtcta
     8461 tagtcggcgc aaagataaag gagcttagat tattaaaaga agatccgaca gacggtgaga
     8521 tggtgatcta tacaacgatc ataaatgatg aagatgaaac tgaagaatta cttgaaaagc
     8581 ttccagcaga tacttcagct aaagataaaa aagcagcagt tgctcttatt gaatctgaag
     8641 cagaaagtgc aagaagttat attgagaaat tattttttaa tacaaaaaaa tatgaccttg
     8701 gtgaagtagg aagatataag atcaactata aacttgagac aaacattgat ccgcatatta
     8761 caatacttac acaggaagat cttgtaaata ttattaatta tataagagac ctcgttgacg
     8821 gaaagaaaga tgtggatgat attgaccatc ttggaaacag aagagtaaga acagttaatg
     8881 agcagttgtc tgcacagttt gcacttggac tttcgagaat gtcaagaact atcagagaca
     8941 agatgagtat tcatgatgaa gaaaatctga caccttctaa acttatcagt gcaagaccga
     9001 tcacaagtgc acttgcttcg ttctttggaa cgagtcaatt gtcacagttt atggatcaga
     9061 caaatccgct tgcggagatg actcataaga gaaggatcag tgctcttgga ccaggcggac
     9121 tttccagaga gagagcggga tttgaagtaa gggacgttca ctatacacat tacggaagac
     9181 tttgtccgat agaaacaccg gaaggaccga acatcggact tatctcgtca ctttgtatcc
     9241 actcaagaat caatgagctt ggattcattg agacacctta cagaaaagtt gaaaatggca
     9301 gagtgacaga taagatagaa tatctgtctg cagagatcga gaattatttc aagatcgcac
     9361 aggcaaatgc tcctatcgat gaaaagggta agtttgtgaa tgataaaata aaaggacgtt
     9421 tcaaaggcga cttcccgtta ttgtctcctg atgaagtgga ttatatggac gttgcgacta
     9481 atcaaattgt tagtgctgca gcggcgttga ttccattcct tgaacatgac gatgctaaca
     9541 gagcgctgat gggaagtaat atgcagagac aggcagtacc gcttctgaga cctgaatcac
     9601 cggtagtcgg aacgggtttt gaagagataa ttgcaagaga ttcgagaaca cttctgactg
     9661 ctgaggcaga cggtgtagtg gaatatgtag acggtaacag aattgtagta aaatataatg
     9721 tcaaagaaga ttcagatgat agtctgctga gttttgatga caggaaacat gttatttata
     9781 atctgataaa atttctcaga acaaatcagg acacatccat taaccaaaga ccaattgtac
     9841 aggaaggtca gaggatcaaa aaaggcgaca tacttgctga cggatcttca actcaaaatg
     9901 gtgagcttgc acttggaaga aacgtacttg ttgcattcat gccgtggaga ggatataact
     9961 ttgaagatgc tattgtgatc agtgagaaat tagtaatgga tgacgtttac acttcgatac
    10021 atattgaaga gttcagtctg gaagtaagag atactaaaag aggcgaagag gaattgacaa
    10081 aagaaattcc taacgtcagt gaagaagcta ctaaagatct ggatgaaaac ggaattgtaa
    10141 gagtaggtgc agaggtaaaa gagaatgaca ttcttatcgg taaagtaaca ccaaaaggtg
    10201 agactgagcc gactcctgaa gagaaacttc tgagagcgat cttcggagat aaagcaggtg
    10261 atgtaaaaga tgcatcgctg aaagctactc ctggactgaa aggaattgtc ataaacaata
    10321 aactcttttc aagaaagaca agagatactg agagtaagag agatgagaaa agaaagatag
    10381 ataaattcga agctgaaaga aagaaagaga taaaagagct gaacaataag atcgcgaata
    10441 agtatgacat acttcttgaa ggtaagacct caaaaggtat gaaagacaaa aaaggaaata
    10501 tccttgtcaa gagcggtgca aaattcaaga gcggtgcttt tgtaaatctg atagagaatg
    10561 aaggatattt attagcggac tttgattatg attctgaatg ggcggattac agcagagcaa
    10621 acactctggt accggacatt tacaggaatt atactttcaa gcagaatgaa atagaagaga
    10681 gatataagag attaaaagat aaggtaaagc tcggcgacga acttccgaca ggcgtagtaa
    10741 agcttgcgaa agtatatgta gccaagaaga gaaaattatc tgtcggtgac aagatggcgg
    10801 gaagacacgg aaataaaggt gtggtcgcga aacttgtacc aatggaagac atgccattct
    10861 taccggacgg aacgcctgtg gatatagtac ttaatcctct tggtgtacct tcgagaatga
    10921 atctcggaca gctttatgaa actgcacttg gatgggctgc aaagaaaatg ggtgtgaaat
    10981 ttgctacacc gatctttgac ggtgctacag ttgcagagat cgtggatatg ttgaaacttg
    11041 caggaatcga tgaaaattca agaacatatt tatttgacgg aatgaccggt gaaagattcg
    11101 atcagagagt tacatgcggt tatatttata tgctgaaact ttcacactta gtggacgaca
    11161 agattcacgc aaggtcgatc ggaccatact cactaatcac tcagcagccg ctcggcggaa
    11221 aggcgcagtt cggaggacag agattcggag agatggaatg ctgggcattg cagggatacg
    11281 gagccgcaaa tattttacag gagatgctta cttataagag tgatgatgtc acaggaagaa
    11341 gcaaggtata cgaagccatt attaaaggcg ataatttgcc tgaaccggga gttccggaat
    11401 ctttcaatgt attactgaaa gagctgcagg gattgtgtct tgatataaat ctggatgagt
    11461 agttttagat aaagtatgca gttttaccga atgtagatta cattcattta ttaaaaaaga
    11521 ttataaagaa aatttaaata agaggtaaaa aatggctttt aaaacagagc acaaaagaaa
    11581 aagattttct aagataacca tcaatcttgc ttcaactgat gcaattatca caaaatcatt
    11641 tggtgaagta ttaaagccgg aaactattaa ttacaggacg ttcaagccgg ataaagacgg
    11701 tttgttctgt gagaagatat tcggtccggt cagagactgg gaatgtcact gcggtaaata
    11761 taaagggatt cgatataaag gaattgtctg cgacagatgc ggagttgaga tcaatctgaa
    11821 aaatgtcaga agagaaagaa tgggacacat tactttaagt gtgcctgtcg ttcacatctg
    11881 gtatttcaga tcactgccta caaagatcgg ttatgtactt ggaatttcct ctaaggaatt
    11941 agagaggata atttattatg aaacacatgt agtgataaat ccgggcaagt caaattataa
    12001 aagacttcag ctaattaccg aagatgaata caatgaagtc ttagatagta tgactgaaga
    12061 agaaatgaat ttagatgatg atgatccgaa gaaattttat gcaggtcagg gcggtgaagc
    12121 tgtcaaagaa cttttgagaa gtgttaatac tgaagaagag atcgcaagat taagagctga
    12181 gcttaaagat gatccgtcag cattgaagaa gatggaaaat atcaagagac tcagaatact
    12241 cgaagcattc agaataaagc cgggcggcag accaaataaa cccgagtgga tggtgctcga
    12301 cgtagtacct gtaataccgc cggagcttag acctcttgta ccgcttgaag gcggaagatt
    12361 tgcaacgagt gatctgaatg atctttacag aagagtaatt atcagaaata acagacttaa
    12421 aagactgatg gacattaaag ctcctgaagt gattttaaga aatgaaaaaa gaatgctgca
    12481 ggaagctgtc gatgcattgc ttgacaactc aagaagagtt acagcagtga aggcagaaaa
    12541 cagagcattg aaatcattgt cagatattct caaaggtaag cagggaagat tcagacagaa
    12601 tctgctcggt aagagagttg attattcagg aaggacagta attgtagtcg gacctgaact
    12661 gaaacttcac cagtgcggac ttccaaaaga catggcattg gaattattca agccgtttgt
    12721 aataagaaga cttatcgaca gggattatgt aaagactgta aagagtgcaa aaaaattaat
    12781 agataagaga acacccgaag tctgggatat attagagaat gtgatcaacg gacatcctgt
    12841 tttattaaac agagctccga cacttcacag actaagtatc caggcgtttc agccgatcct
    12901 tattgaagga aaagctatta cgcttcatcc ttttgtttgt attgcattca atgctgactt
    12961 tgacggtgat cagatggcgg ttcacgttcc actatcatat gaggcacagc ttgaagcgca
    13021 gatcatgatg ctttcttctc acaatatact ttcacctcaa aacggtgctc ctattactat
    13081 tccgaatcag gaattaatat taggatgtta ttatcttaca aaagaattgc cgggagatct
    13141 gggagaagga aaaatatttt caaattcaga tgaagtaatg ctggcatttg acaataaaaa
    13201 agtcggactg catgcaagaa ttaaagtcag attcagaggc gaaataatcg agactacagt
    13261 tggacgggtt atttttaata gatgcgtacc tgagaagatt ccatttatca atgaacttct
    13321 gaaaaagaag aagatcattg aaatgataaa tatgatttat aatagagtgg gaaatctcga
    13381 gacttctgta ttccttgata atctgaaaga gatcggattt aaatatgcaa caatgggagg
    13441 actttctgta aacattgatg atattgaagt acctgcaacc aaagacaaga tcattgaaga
    13501 ggcattcctc agagttgaca gaattgaaaa atcaaaagca aaaggtataa tttcagagaa
    13561 tgagagatat aataaagtta ttgatatatg gacacacgta cgggatgaag taaagagaga
    13621 tctgatgaga aatttattga gctccaagca gggatttaac tcattgcaca tgatgattga
    13681 ttcaggtgcg agaggttcgg cagagcaggt cagtcagctc gcaggtatga gaggacttat
    13741 gcagaagccg cagaaatcca tgaccggtca ggcaggtgag atcattgaga atccgatcat
    13801 tgcaaacttt aaagaaggtt tgtcaattct cgaatacttt atttcaactc acggtgcaag
    13861 aaaaggtctt gctgatacag ctttaaaaac tgctgacgct ggatatctga cgagaagact
    13921 tgttgatgta gctcaagatg ttattattat agaagtggat tgtaacacta tcagaggtgt
    13981 aactactgaa gcactaaaag aaggagaaga cattaaggaa tctctggctg aaagaattct
    14041 tggcagagta gcagttcatg atgtggtaaa tccgctgact aaaaaagtaa tcatcaaagg
    14101 cggtgaactt attaatgaag agcttgcaga catggtagct gaaactccta tcacagaagt
    14161 tgagatcaga tcagtattga catgcgaatt aaaaagaggt gtctgcgcaa aatgttacgg
    14221 cagaaatctg gctacaggaa agatggtgga agtaggagaa gcagtaggaa tagtagctgc
    14281 acagtccatc ggtgagccgg gtacacagct tactttaaga acattccata tcggtggtac
    14341 tgcaagtaag atcatttcgc agtcgcagat ctctacgaaa tttgaaggta aagtgaaatt
    14401 tgaaaatatt aagatcgtag aatataaagg cggagctgga acggaatttg tttctcttag
    14461 ccgaaacggt ctgataaata taatggatga aaacggttca cagcttgcta gatatactgt
    14521 gccttacggt acacatctca aagtaaaaga caaagagata gttcctaaga atgcaattct
    14581 gtatgaatgg gatccgtata actctgtaat tgtagctgaa gatgatggtg ttataaatta
    14641 ttttgaaatg gatgaaggca agcagtatga tatttcaaaa gatgaacaga caggattctt
    14701 acaaaagatc gtaattgaat ccagagataa aacgaaatca cctacgattc agattttgaa
    14761 tccgaagaat gaaaagctta tgagttctta tctgatacct gccaaagctt cactgttggt
    14821 agataacgga gctgatgtaa aagccggaac catcatggta aagatcccga gagagtcggg
    14881 taagacacgt gatattacag gtggtcttcc gagagttact gaattattcg aagcaagaag
    14941 tccaaatgat ccggcaaaag tcgctgagat caatggtact gttgagattg gaaaaatttc
    15001 aagaggcagc agagaagtaa ggatcatcag taaagacgga agtaaagtta ttgattataa
    15061 aatacctatt gggaaacata ttcttgtaag acacggagac atcgtgaaat caggagagcc
    15121 gctcacaggt ggtgcttatg atcctgttga tattctgaag atcaaaggtg ttgctgaagt
    15181 tcaggaatat cttgtaa
//
LOCUS       DAORVQ010000073        14902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1797_length_14902_cov_4.919512, whole genome shotgun sequence.
ACCESSION   DAORVQ010000073 DAORVQ010000000
VERSION     DAORVQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14902
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1797_length_14902_cov_4.919512"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLX80_07540"
     CDS             complement(<1..755)
                     /locus_tag="PLX80_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20674.1"
                     /translation="MNILYSKWREDSMTDESRLENLISMFSYLLIQTNGDVEEALEWM
                     EELNDSYKLFDKDMTFSNFVDELKKQGYIAEQEGMKIITAKGSQRIRSESLRKIFSGL
                     KPGTTGFHESTKSGRGVDKLNEIKKFEFGDQVTNIDMTSTFKNAFLKSDINNLRLSED
                     DIDVFETEHMTSCATVLMIDISHSMILYGEDRITPAKEVALGLSELILSKYPKDKLNI
                     VVFGDDAREISVKDLTFLSVGPFYTNTQAGLRLA"
     gene            complement(774..2216)
                     /locus_tag="PLX80_07545"
     CDS             complement(774..2216)
                     /locus_tag="PLX80_07545"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQY20675.1"
                     /translation="MIQLHEIYEVIPIVLIAFAACAILLIEAFSKESKSVVYGFSIIS
                     IMGAIATSFLSLNKEVIVFNQFLKISNASIMFSVIILIGVLITVIASKNYLEKENINF
                     GEFYSLVLFSLMGMMLMVFANDLLIIFIGLELMSVCFYVLAGFLRLRKKSNESALKYF
                     LLGAFITGFILYGIALIYGITGTTNLTRIFADNKIFSNYVFIIGYALFLIGFLFKIGV
                     FPFHMWIPDVYDGAPTIVSGMLSTAGKIAAVGTVLPIILTVNIVSFKLIFSVAAVATM
                     MYGNIIALAQTNIKRLLAYSSIASAGYIFVGVAAMNEFALKGIAYYLIAYTFMQLGAF
                     IIVSILEKKDDGSREYKYVDLDFYKGLGKRNPVLATFLTIFLFSLAGIPPFAGFWGKY
                     YLFYAAIQANLIWLSVIGILLSLIGVYYYLKITVYMWFSESTEELMAEYEKVPVSRSG
                     IFATSLAVIGTIVFGLDPNLFFRIFKMIAE"
     gene            complement(2227..3057)
                     /locus_tag="PLX80_07550"
     CDS             complement(2227..3057)
                     /locus_tag="PLX80_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQY20676.1"
                     /translation="MKIKTKYIHYGLIFFCLLIFTESSIPGDKLPKAEFEYSDKFIHA
                     AIYFILYILFFYSLKYQTKSVKLHRFSFEFALIFTVLYGATDEIHQYFVPNRSCEFYD
                     WIADIAGAVIGLITVKYFLLKKINIAVLFLLIFVIGCNASDTKDNDDSTNVKEMYITE
                     ADSWIDKMPGVGDVNHKFGFSISISIQTDSKPEDFEVRNLVIYLNNDTLRSAPYETDI
                     FESSKGNTNINVRHDRNHIYLDPDKSEPVESKFEFDIYSKNKKSGKIKTDKFKILTVY
                     "
     gene            complement(3054..4625)
                     /locus_tag="PLX80_07555"
     CDS             complement(3054..4625)
                     /locus_tag="PLX80_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQY20677.1"
                     /translation="MKNVFFNSEVLDAESKIISSLKIPSVILMENAGKNFTKILLNRI
                     SEKDSSINNERNFIVLTGKGNNAGDGFVIARHLCNQDFNVTLVMLFEEKELQSDAAVN
                     FSVLKNSKTISENLKIYRFKNISDLKKKISFGNSIIIDAIFGVGFRGAPDKKMTHLFK
                     FLNQIKSKTLFSVDVPSGLSHYNQDTELFKADVTISMGVRKFHTLFYKGRENSGEIET
                     ADIGISENEFTNFNIKKTKITEEKDVRSFIPERKKNSNKYTNGKVLVIAGSKGLTGAV
                     YLSSVSAMKTGSGAVIAAVPESVNDILEIKMTEVMTLPVSETSDSALSLNSYDKIKTK
                     IKWADTILIGPGLSKNEETMELVRYIVKNNKSRFVIDADAIYAFRGNMNLLKDREIII
                     TPHFGEFSSLTGIDLDELKNNFYEISKEFAVKYKVCLVLKNSPTIITDGNEFIINPTG
                     RENLATAGSGDVLSGIISALFSQTGNAMGSAVSGTYLHGKCGDILFQKYGSSSTLASD
                     IINEIPFVKNQISSA"
     gene            complement(4654..5304)
                     /gene="rpe"
                     /locus_tag="PLX80_07560"
     CDS             complement(4654..5304)
                     /gene="rpe"
                     /locus_tag="PLX80_07560"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQY20678.1"
                     /translation="MKKLLPSILAADFSKLGDEIKEVESYGADIIHCDIMDGHFVPNI
                     SFGPKIISDINKITDLPLDVHLMIDNPQDFIVKFFEAGADMISVHYENNTHLDRVIQS
                     IKDLGIKAGVVLNPATPVFMLEDILGLVDYVLLMSVNPGFGGQKFIDNILRKISILKN
                     MIEKKGYDIEIEVDGGIDLTNIKLISEAGADLFVCGSSIFNSTDRKEVMSKMRSLI"
     gene            complement(5321..6247)
                     /locus_tag="PLX80_07565"
     CDS             complement(5321..6247)
                     /locus_tag="PLX80_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQY20679.1"
                     /translation="MNSVIMPLYVKHSDTVTTPKVTGTNYLEAKKVIEEAGLEIIQGD
                     IKYDENIQIGLIIDQNPPPEATVKYGRRIYVTISGGDQLSEVPNLIGKSLRDAKFTLE
                     QRGLELGETVRKSTNDFPEDFVISQIVQPGSKVKRQTKLDLILSDGPSIGDLKVPNVV
                     GKTLDDAKKLITESKLKIGKITYQTNNNVPPGQIVDQYPKSDKSANESTSVDIFVARK
                     KVIEEPEVIDEDNSNKKENDPQLKNESDTNTDENKSDSEKPLIKKDKSTDTKDKPSTN
                     KPDKDKSDTKEDTKKNSPDTKSTEDKGDEIPK"
     gene            complement(6428..7456)
                     /locus_tag="PLX80_07570"
     CDS             complement(6428..7456)
                     /locus_tag="PLX80_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20680.1"
                     /translation="MKFVILKIFLLFLFWNANYNFATELSSSGQVTDVLIIKNDNGTS
                     ADQIKIDLEQAKYNVNMIAPELVTPEMFNEHRLVILSTGSNLFPCTDNNMRFLLQSFI
                     DDGGKVIVEGGQTGYVSDVIPEYPAFKSKVMKIDSWQNDNGGSIIISNEYRNSMLATF
                     PNILKDSINIKYSGQADMDVCAKNEFSKLFYKTEMYPDKLGILVFPNVLTPQIINLFF
                     CYSRIDDISSAKHLIINCAYNLIGKPIGIEVVSTEIPEKFFLHQNYPNPFNPVTVIRY
                     DIKETDVVSLKIYDVLGSEKSLIVNEKQSPGSYKIIFDGSFLSGGVYYYKLTSGNFSD
                     TKKFVLIK"
     gene            7596..8093
                     /locus_tag="PLX80_07575"
     CDS             7596..8093
                     /locus_tag="PLX80_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HQY20681.1"
                     /translation="MAAQFSFDIVSEVDLQELDNAINQALKEILQRYDFKGSKSEITF
                     NKKEKVLNIISDDDYKLKAVVDILQNKMIKRGISIKALKYGNVEPAGGNTVRQEIKLQ
                     QGIEKDDAKLIVKLIKDAKLKVQATIQDEQVRVSGKVKDDLQEVMNLLKEKELDFALQ
                     FTNYR"
     gene            complement(8131..8994)
                     /locus_tag="PLX80_07580"
     CDS             complement(8131..8994)
                     /locus_tag="PLX80_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQY20682.1"
                     /translation="MHDSEIIAKTRDVDPGYTQEGEASWYGPGFQGRKTANGERFDTH
                     EMTAAHKTLPFNTLVKVTNLSNDISTVVRINDRGPFIRGRIIDLSKAAKQEIEMGGLA
                     HVRIEIYNPEEEEETESEISNSQVNLFEDEFPANSKVFVQLTEDSGENSEYTLSKNEL
                     NILFNSSKIKIKVLTSDVEFVDTKIYQEVSDNSSYNYFDVTKKIRFIKGFTIQVARLI
                     DAESAEQLITKLESENFNTIFLEEVVSKDNTYFKVYVGNYENLESTNEDINRLIAFDN
                     DLKLKILKIGS"
     gene            complement(9379..11544)
                     /locus_tag="PLX80_07585"
     CDS             complement(9379..11544)
                     /locus_tag="PLX80_07585"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20683.1"
                     /translation="MKKFIYFLFTAFLCLTIGNKSSFADGLNSVHTSNATFVVAAGNQ
                     GNILYSNTTGNTWSKTTIPGIDFKSVFALNNNVWYTASNGKVYTSSNSVTDIIQRNTG
                     IATSLNSVYFINSTNGFVCGDNGVILKSVDGGITWTLSNSGVSIENLNSISFKDAQNG
                     VAVGNNGSVFITTNGGSSWSAETVGTTRDLLAAKYFSDGIGIAGEWGTVLYRSLVSTW
                     NSPNMKIKSDIRGITGDSFNDIHVCGGGGFIRNNINGNTEFLNFEKNPMLADLVSISS
                     FSGIGFAVSSMNNAVIRTTDGGVTWNLPSGTAVSYNWVSKPGASGDFLGNNMSLHPTE
                     RNTIFIAFGNKVYRSLNKAESWSEIGNQIPSGSTPHSFFVSPADTNIWLVAIQSSPDK
                     VYRTTNYGQSWTEVMSRNFSNYGQPLEMDQNDPHVFYFAPDNGGFWKSTDDGATWNEI
                     SNNFSFRSPCDIIVMHDSSNVIFIADGVTGSGQAKIFKSVNGGVNWTDVHTANSSEIP
                     SMSNTIFDKSIIWATEWSGSRVYKSTDFGDTWAQNHTTGFSGWGSDICREDPNLIITG
                     SWGSAATMSLDGGVTWTNISAGLNGHGGGILIPDRGYMVCHQGSNVYKLNVVYTVLTS
                     VSENIISTVPVDFNLSQNYPNPFNPSTKINYNLPKSDNVSIKIYNELGKEVSTLVSGF
                     KTAGTYEITFDGANLSSGIYFYKLQTTGFTATKKMLLIK"
     gene            11799..14261
                     /locus_tag="PLX80_07590"
     CDS             11799..14261
                     /locus_tag="PLX80_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20684.1"
                     /translation="MTLTRSILFIFSFLIITSDIYAQTFNYPIVFVSRNHETNGNIFY
                     PDAGLLPGMGPFSRFSVVGGKLMVRDKQGVITTLLDSSITLNGIRLIDFQQPCVNWSG
                     DKILFAGIEDIDSSWRIYEIFSDGSRMRKITFTNRNIDLLQFGPAGHKFRTYEDIDPI
                     YLPDGSIIFASTRYPTLSEIGGFQTTNLFIADSLGNNIRRVTSERNGGEKPTIDPVSG
                     RILYSRWWVNIDKPSNVTSSGLTRIDSLALSQDIANIWQVDIVNPDGDALKLFATDPR
                     NRNSLSSYRPRVAANGKLLTVFIPSLPIVTTGGSTGIRFYEQGFSEAKKIIGTDSTTP
                     LYIQNPPSTGTMQPPYATDPLGLPDGSVLFSYASTVEAQDYGIYTVNINGTGLTQVVD
                     FPGTLELNAELLIPRKVPPKVFYLNDFDTNNVPPTSNPNSFYQGGLFRFDCLNVYSNA
                     PVDSPIGDGPPIKKNARFRFFLNFQRENENGQDLPILFREIALDRDGKIAEGDIPANV
                     SMFEQIIDSTGNVLQNKKGDFAHVTGMNFGNNGSGTKCVGCHAGHTMITVPINLTEGT
                     FTNLSTSADVRESSFKTNGNFAYKGQNVVDRKARNPDQSVNWISNGTINEFVVLKWEI
                     PIDIRRIKLYDILPNQNNNTDIHVTDCEIFFYYENSVANHIQSTGPLNTDGLELNVLP
                     ITTIDSAKIIVKSFTGNINNIQSAGLAEVEVNARVSSIDLVNISNNEIALYDYKLEQN
                     YPNPFNPSTIINFTLPKAQTISLEVFDITGRNIATLINGRVEGGRNSITFNAANLPSG
                     IYFYRMTAAGKFVEAKRMILLK"
     gene            14340..>14902
                     /locus_tag="PLX80_07595"
     CDS             14340..>14902
                     /locus_tag="PLX80_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQY20685.1"
                     /translation="MENKSNKNNIGQLQGKLLISAPFLDDIFKRAVILITENNDEGSV
                     GFILNKITEYKLHEIIEEFPEFDAKVFIGGPVQQEALNFIHKAGDILEGSFQISEGVF
                     WGGNFEHLKILIENNAVDPDDFRFFLGYAGWGNGQLENELKLKSWYVNDSLNDNIFSH
                     NSANLWSKVLRSMGNEYTVISTFPEDPS"
BASE COUNT         4848 a         2610 c         2275 g         5169 t
ORIGIN      
        1 gccagtctga gccctgcttg tgtatttgta taaaacggac ccacagataa gaatgtaaga
       61 tcctttacag aaatttctct tgcatcatct ccgaagacta caatattcag tttgtctttc
      121 ggatacttag aaagaataag ttctgataat ccaagtgcaa cttcctttgc aggagtgatc
      181 ctgtcttctc cataaagtat catgctgtga gatatatcga tcattaaaac tgtagcacag
      241 cttgtcatat gctcggtctc aaatacatca atatcgtctt cggaaagtct taaattattt
      301 atatctgatt ttaagaatgc atttttaaag gtagatgtca tatcgatatt tgtaacctga
      361 tcaccgaatt cgaatttttt aatttcattt aatttatcaa ctcccctgcc tgacttcgtt
      421 gattcatgaa atcctgtagt tcccggcttt aatcctgaaa aaattttcct caaagattct
      481 gatctgattc tttgagaacc ttttgctgta ataatcttca ttccttcctg ttcagcaata
      541 taaccctgct tttttagctc atctacgaaa ttactaaatg tcatatcttt atcaaaaagt
      601 ttataactgt cgttcagctc ttccatccat tccaaagctt cttctacatc accatttgtc
      661 tgtataagta aatagctgaa cattgaaatc agattttcaa gtctgctctc atccgtcatt
      721 gaatcttcac gccattttga ataaagaata ttcatttttt aaatatagtt aaattactca
      781 gctatcattt taaatattct gaaaaataaa ttcggatcta atccaaatac aattgttcca
      841 atgactgcaa gtgaagttgc aaaaatacct gacctgctta caggtacttt ttcatattca
      901 gccattagtt cttctgtgga ttcactgaac cacatataaa ctgtgatctt taaataatag
      961 taaacaccta tcagacttag taaaatacct atcactgaaa gccagatcag atttgcctgt
     1021 atcgctgcat agaataaata atattttccc cagaatccag cgaaaggcgg aatgcctgca
     1081 agtgaaaaaa gaaatattgt cagaaaagta gcaagtaccg gattacgttt tccaagtcct
     1141 ttatagaaat caagatctac atatttatat tctcttgagc cgtcatcttt cttttcaaga
     1201 attgatacaa ttataaaagc accaagctgc ataaatgtat aagcgatcag ataatatgcg
     1261 attcccttta gtgcaaactc attcatcgca gctacaccga caaaaatata tcctgcactt
     1321 gcaattgatg aataagctaa aagtcgttta atatttgtct gagcgagggc aattatattt
     1381 ccgtacatca ttgtcgctac agcagctacg gaaaaaataa gtttaaagct tacgatattc
     1441 actgtcagaa ttatcggaag tacagtacca actgcagcaa ttttaccggc tgttgacagc
     1501 attccggaaa cgatcgtcgg tgcaccgtca tacacatcag gtatccacat gtgaaatgga
     1561 aatacaccga tcttaaataa aaagccaatc agaaacagag catatccgat tataaagaca
     1621 tagttggaaa aaattttatt atcagcaaat atcctggtta aatttgttgt ccctgtgata
     1681 ccatatatca gagcaattcc atacaaaata aaaccggtaa tgaacgctcc gagtaaaaaa
     1741 tatttcaatg cactttcatt agattttttt ctcagtctca aaaatcctgc aagtacataa
     1801 aagcaaactg acatcagctc aagtcctata aaaattatca aaagatcatt agcaaaaacc
     1861 atcagcatca ttcccattaa agaaaataat accagtgaat aaaattctcc gaagtttata
     1921 ttttcctttt ccagataatt ttttgaagca atgaccgtga tcagcacacc aattagaata
     1981 attacactga acataatgga tgcatttgaa atcttcagaa actgattaaa cacgataacc
     2041 tctttattca gagaaaggaa ggaagttgca attgctccca ttatactgat tatactgaat
     2101 ccgtaaacta cacttttact ttcctttgaa aaagcttcga tcagcagaat tgcgcaggca
     2161 gcaaatgcta tcagcacaat aggtattact tcatagatct catgtaattg tatcatttat
     2221 tttttattaa tagacagtta atattttaaa tttatcagtt ttgatttttc ctgatttttt
     2281 atttttagaa tatatatcaa attcaaattt agattctacc ggttctgatt tgtccggatc
     2341 aagatatata tgattccgat catgtcttac atttatattt gtgtttcctt ttgaagattc
     2401 aaaaatgtca gtctcataag gagctgatct caaagtatca ttattcagat aaatcactaa
     2461 atttcttact tcaaaatctt cgggtttgct atcagtttga atacttatgc ttattgaaaa
     2521 tccgaattta tgattaacat cacctactcc cggcatttta tctatccagc tatccgcctc
     2581 agtaatatac atttctttta catttgtgct gtcatcatta tcctttgtat ctgaagcatt
     2641 acagccaatc acaaagatca gtaaaaataa aactgctata ttaatttttt tcaataaaaa
     2701 atattttaca gttattaaac caattactgc ccctgcaata tcagctatcc agtcataaaa
     2761 ttcacagctt ctgtttggta caaaatactg atgaatctca tctgtcgctc cgtaaagcac
     2821 agtaaatatt aatgcaaatt caaatgaaaa acggtgtaat ttaacacttt tagtttgata
     2881 ttttaaggaa taaaaaaata atatatatag aataaaatat atggctgcat gaataaattt
     2941 gtcactatat tcaaattctg ccttcgggag tttgtcaccg ggtatagatg attctgtaaa
     3001 aattaataaa caaaaaaata tgagaccata gtgtatgtat tttgttttga tcttcaagca
     3061 gatgaaattt ggtttttaac aaacggaatt tcatttatga tatctgatgc taaagtagat
     3121 gaacttccgt atttttgaaa taaaatgtca ccgcattttc cgtgcaagta tgttcctgat
     3181 actgcgcttc ccattgcatt accggtttgt gaaaataatg ctgaaataat tcctgataat
     3241 acatctccgc ttccagcagt ggcaaggttt tccctgccgg taggattaat tataaattca
     3301 ttcccatctg taattattgt tggtgaattt ttcaaaacta aacagacttt atatttaact
     3361 gcaaactctt tagaaatttc ataaaaatta ttctttaact catctaagtc aataccagtt
     3421 aaactggaaa attcaccaaa atgcggagtg ataattatct cacggtcttt taataaattc
     3481 atgtttcctc tgaatgcata aatcgcatct gcatcaatta caaatctgga tttattattc
     3541 tttacaatat atctcacaag ctccatggtc tcttcatttt tagacaaccc gggtccaatt
     3601 aaaatagtat cagcccattt tattttggtt ttgatcttat cataagaatt cagacttaga
     3661 gcagagtcac ttgtttcact aacaggcaga gtcattacct ctgtcatttt tatttcaaga
     3721 atgtcattca ccgactcagg aacagcagca atcacagcac ctgatcctgt tttcatagct
     3781 gaaacagaag ataggtaaac agctcctgtg agaccctttg aacctgcgat cacaagtact
     3841 ttgccatttg tatacttatt cgaattcttt tttctttcag gaataaaact tcttacatct
     3901 ttctcttctg taattttagt tttctttata ttaaaattgg taaattcatt ttctgaaata
     3961 cctatatcag ctgtttcaat ttctccgctg ttttctctgc ctttgtaaaa aagtgtatgg
     4021 aattttctca ctcccataga gatagttaca tcagctttga ataattctgt atcctgatta
     4081 taatgactca aacctgacgg aacatcaaca gaaaacagag ttttagattt tatctgattg
     4141 agaaatttaa atagatgtgt cattttttta tccggcgcac cccggaaacc tactccaaat
     4201 attgcatcta taattatact gtttccaaaa ctgatcttct ttttaagatc tgaaatattt
     4261 ttaaatctgt agatctttaa attttctgaa atagttttgg aatttttgag aactgaaaaa
     4321 ttaactgctg catcactctg aagttctttt tcttcgaata acatgacgag tgttacatta
     4381 aagtcttgat tacataaatg cctggcgatc acaaacccgt cacctgcatt atttccttta
     4441 cccgttaata caataaaatt tctttcatta ttaatagaag aatctttttc agatattcta
     4501 tttaataata ttttggtaaa attttttccg gcattttcca ttaagattac tgaagggatt
     4561 ttcagagagg agattatttt actttctgca tcgaggactt cgctattgaa gaatacgttc
     4621 ttcattttat taataatctt aaaatcaatt tattcaaatt aacgaccgca tttttgacat
     4681 aacttctttt ctgtctgtgg aattaaaaat tgatgatccg cagacaaaaa gatccgcacc
     4741 tgcttcagag atcaatttaa tatttgtaag atcaattcct ccatcgactt caatctcaat
     4801 atcataacct tttttctcta tcatattttt taaaatgctg attttcctca gtatattatc
     4861 tataaatttc tgacctccaa aacccggatt tacactcatt agcaatacgt aatccaccaa
     4921 acccagaatg tcttccagca taaaaactgg cgtcgcagga tttaacacaa ctccggcttt
     4981 tattccgaga tctttaatgc tttgtataac tctgtcgaga tgagtattat tttcataatg
     5041 aactgaaatc atatccgcac ctgcttcaaa aaattttaca atgaaatctt gcggattgtc
     5101 aatcatcaga tgaacatcta atggcagatc agtgatctta tttatgtctg atattatttt
     5161 aggaccaaag ctaatatttg gcacaaaatg tccatccata atatcacagt gaatgatatc
     5221 tgccccgtat gattctactt cttttatttc atcaccgagt ttagaaaaat cagcagcaag
     5281 aatggaagga agtaattttt tcattattgt ttaagtgaaa ttatttcgga atttcatctc
     5341 ccttatcttc agtagactta gtatcaggtg aatttttctt agtatcttct tttgtgtcag
     5401 atttgtcctt atcgggttta tttgttgatg gtttatcttt agtatctgta cttttatcct
     5461 tcttaattaa cggtttttca gagtcagatt tattttcatc agtattggtg tcagattcat
     5521 ttttcagttg cgggtcattt tctttcttat tagaattatc ttcatcaatt acttccggct
     5581 cttctatgac cttttttctc gccacaaaaa tatcaactga agtactttca tttgccgatt
     5641 tatctgactt aggatattga tctactatct gtccgggagg tacattatta ttggtctgat
     5701 aagttatctt tccaatctta agtttacttt cagttattag cttttttgca tcatcaagcg
     5761 tcttgcctac tacattgggc actttcagat ctccaataga aggtccgtca cttaatatca
     5821 gatcaagttt tgtctgcctt ttcactttac ttccaggctg tactatctga gaaattacaa
     5881 aatcctcagg aaaatcattt gtactttttc ttacagtttc accaagttcc agccctctct
     5941 gctcaagagt aaactttgca tcacgaagtg atttaccaat aagattcgga acttcagaaa
     6001 gctggtctcc tccgcttata gtaacataaa ttcttctccc atattttact gttgcttcag
     6061 gaggagggtt ctgatctatt attaagccaa tttggatatt ttcatcatat tttatatcac
     6121 cttgtataat ttcaagaccg gcttcttcaa ttactttttt tgcttcaaga taattagtac
     6181 ctgttacttt gggagtggtg actgtgtcag aatgttttac atacaaaggc atgataacac
     6241 tattcatcaa aatgaaaaag aatactataa tcccgaagaa aattagaagt tttttaagca
     6301 tgcaggtaaa ttaataatat gaactctgaa atgaaatgaa ttttaacagt tatttagttt
     6361 tgaattttaa ttttggatta tgaatatctc aatttaataa aactaagatt ttatttccaa
     6421 aacaatttta ttttattaat acaaattttt ttgtgtcaga aaaattacct gaagttaatt
     6481 tataataata aactccaccg gataaaaaac ttccgtcaaa aataatttta taacttccag
     6541 gactttgctt ttcatttaca attaatgatt tttcactacc taatacatca taaattttta
     6601 aactcacaac atcagtttct ttaatatcat atcggattac tgtaaccgga ttaaatggat
     6661 ttggataatt ttgatggaga aaaaattttt cgggaatttc agtacttact acttcaattc
     6721 caatcggctt tcctatcaga ttataagcgc aattaataat aagatgcttt gcactgctta
     6781 tatcatcaat tctggaataa caaaaaaata gattaatgat ctgcggtgtc aatacattgg
     6841 gaaatacaag tatgcccaat ttatcgggat acatttcagt tttataaaaa agttttgaaa
     6901 attcattttt tgcacagaca tccatatctg cttgtccaga atatttaata tttatagaat
     6961 ctttcaaaat atttggaaaa gtagcaagca ttgaatttct atattcattt gaaattataa
     7021 tcgatcctcc attatcattc tgccagctgt caattttcat gaccttactt ttgaatgcag
     7081 gatattcagg aattacatca gacacgtaac cagtctgtcc tccttctacg atcactttac
     7141 caccatcatc aataaaactc tgcaaaagaa atctcatatt attatctgtg cagggaaaaa
     7201 gatttgaacc ggtagaaaga atcaccagtc tgtgttcgtt gaacatttcg ggagtgacaa
     7261 gttcaggtgc aatcatgttc acattatatt tcgcctgttc aagatcaatt tttatttgat
     7321 cggcggaagt tccattatca tttttaatta tcagtacatc tgtaacttgt ccggatgagg
     7381 acaattctgt tgcaaaatta taattcgcat tccaaaaaag aaacagcaag aatattttca
     7441 gtattacaaa tttcataatc taatttaata gtataaataa tttagcaaat ttaattttta
     7501 aacttaactg attatttaat ctgaaaaatt tttcaactta atttagcaat tcaatttaga
     7561 catctaattt gttaagttaa aataaaaact catttatggc agctcaattt tcatttgata
     7621 tagtttcaga agtagatcta caggaactcg acaatgcgat caatcaggca ttgaaagaaa
     7681 ttttacaaag atatgatttt aaaggcagta aaagtgagat cactttcaac aaaaaggaaa
     7741 aagtcctcaa tattatttca gatgatgatt ataaattaaa agcagtggta gatattcttc
     7801 agaataaaat gattaagaga ggaatttcta taaaagcttt aaaatacgga aatgtagaac
     7861 ctgccggagg gaacactgtc agacaggaaa tcaagcttca gcagggaatt gagaaagacg
     7921 atgcaaaatt aatagtaaaa cttattaagg atgcaaaact aaaagtacag gcgacaattc
     7981 aggatgagca ggtaagagtt tcaggtaaag tgaaagacga tctccaggaa gtcatgaatc
     8041 ttttaaaaga gaaagaactt gattttgctt tacagtttac aaattacaga taaaaaaaag
     8101 ccgttgaaaa aatttccaac ggcttaaatt ttaagaacct atttttagta tcttcaattt
     8161 caaatcatta tcaaatgcaa ttaacctgtt aatatcttcg tttgtgcttt ccagattttc
     8221 ataatttccg acatatactt taaaatatgt attgtcttta cttactactt cttctagaaa
     8281 gatagtatta aagttttctg attcaagttt tgtaatcaat tgttctgcac tttcagcatc
     8341 aattaacctg gcaacctgta ttgtgaaacc tttgatgaat ctgatttttt tagtgacatc
     8401 aaagtaatta tagctgctgt tatctgatac ttcctgatag atctttgtat cgacgaattc
     8461 gacatcagaa gttaatactt ttattttaat tttagatgaa ttaaataata tgtttaattc
     8521 attctttgat aaagtatatt ctgagttttc tccggaatct tcggttaact gaacaaatac
     8581 tttcgagtta gccggaaatt catcttcaaa aagattgact tgtgagttac taatctcact
     8641 ttcggtttct tcctcttctt caggattgta aatttcaatc cttacatgag caagaccacc
     8701 catttctatt tcttgttttg ctgccttaga gaggtcaatt attctacctc ttataaaagg
     8761 tcctctatcg ttgattctca caacggtaga tatatcatta gaaagattag ttacttttac
     8821 taatgtattg aatggtaaag ttttgtgggc agccgtcatt tcatgagtgt caaatcgctc
     8881 accattggcg gtttttctac cttgaaatcc tggaccatac cacgaagctt caccttcctg
     8941 agtgtaacct gggtcaacgt ctcttgtctt agcaatgatt tcagaatcat gcatccaact
     9001 gattaagtta attaaaacta atgcgaatat taatttcgca gcaattttaa cgttcaatgt
     9061 gtatctccta tttagtttat aaatgagttt atgtaacact cttagagctg ttaggtcagc
     9121 tcaaaatcta acttaatctt cgttgtttat attaaagaac gatattgtta ttaaaatctt
     9181 ttaaaatcga atcactaata taagggtttt gatttttaat tgcaaaggtt tttttatacc
     9241 caagacaccc cgtaatttat ttttgactac tgtttaattt tctcatcttt cagaaatcag
     9301 aattattaat gccatatatt tgaatgaata attctttcat gtctgaaaga ttaacaattt
     9361 ctgaggggac atatgtcact attttattaa taacattttc tttgtcgctg taaatccggt
     9421 tgtctgtaac ttataaaaat aaattcccga tgaaagattt gctccgtcaa atgtgatctc
     9481 ataagtacca gcagttttaa atccgcttac caatgtacta acttctttac ctaattcatt
     9541 ataaatttta attgaaacat tgtcagattt tggaagattg taatttatct tggttgaagg
     9601 attaaaagga tttggataat tttgtgacaa attaaaatca actggaaccg tagaaattat
     9661 gttttcactt actgaagtca aaactgtata tacaacatta agcttatata catttgaacc
     9721 ctgatgacat accatataac ctctgtcggg tataagtatt cctccaccgt gaccgttcag
     9781 tcctgcactg atatttgtcc aggtaacacc gccgtcgaga ctcatagtgg cagctgagcc
     9841 ccagcttcct gtaattatta aatttggatc ttctctgcaa atatcagatc cccatccgga
     9901 aaaaccggtt gtatgatttt gtgcccacgt atctccgaaa tcggttgatt tataaactct
     9961 gcttccgctc cattcagttg cccatataat agatttatca aaaattgtat ttgacattga
    10021 tgggatctca cttgaatttg cagtatgtac atcagtccag tttactccac catttacaga
    10081 tttaaatatt tttgcctgac cgctacctgt tacaccatct gcgataaata ttacatttga
    10141 actgtcgtgc attactataa tatcacatgg acttctgaaa gagaaattat tgcttatctc
    10201 attccatgta gctccgtcat cagttgattt ccaaaatcca ccgttgtcag gtgcaaaata
    10261 aaatacatga ggatcgttct gatccatctc aagaggctgt ccataattac tgaaatttct
    10321 tgacattact tcagtccagg actgaccata atttgtagtt ctgtaaactt tatcaggtga
    10381 agattgaata gctacaagcc agatattagt atcagcagga cttacaaaaa aagaatgtgg
    10441 cgtacttccc gatggaattt ggtttcctat ttcactccat gattctgctt tgtttaaact
    10501 tctgtaaact ttatttccaa aagcaataaa tattgtattt ctttcagtcg gatgaagact
    10561 catgttattt ccaaggaaat ctccgctggc accgggctta gatacccagt tatatgatac
    10621 tgctgtgcct gatggcagat tccaggtgac acctccatca gtagtcctta ttacagcatt
    10681 attcatactg ctgactgcaa aaccaatacc tgaaaaactt gatatgctaa caaggtctgc
    10741 gagcattgga tttttttcaa aatttaaaaa ttccgtattt ccatttatat tatttctgat
    10801 aaaaccgcct ccaccgcaaa catgaatgtc attaaatgaa tcaccagtta ttccacgtat
    10861 atctgattta attttcatat tcggggaatt ccaggttgat accaaactcc tgtaaagaac
    10921 tgttccccac tctcctgcta ttccaattcc atcggaaaaa tattttgctg caagtagatc
    10981 tcttgtagtt ccgactgttt cagccgacca tgaagaaccg ccgtttgttg ttataaatac
    11041 tgatccatta tttcccacgg ccactccgtt ttgtgcatct ttaaatgaaa ttgaattcag
    11101 attttcgatt gaaactccag agttacttaa tgtccaggtt attcctccgt caacagattt
    11161 taaaataaca ccattatctc cacagacaaa tccatttgtt gaattaatga agtatactga
    11221 atttaatgat gtagcaattc ctgtatttct ttgtattata tcagtaacgg aatttgaaga
    11281 tgtgtacact tttccatttg aagctgtata ccacacattg ttatttaatg caaaaacact
    11341 tttaaaatca ataccaggaa ttgtggtctt ggaccaggta tttcctgtag tgttagaata
    11401 cagaatattt ccctgatttc ctgcagctac tacaaatgtt gcatttgatg tatgaacact
    11461 gttaagtcca tctgcaaatg aacttttatt tccaattgtc agacatagaa atgctgtaaa
    11521 caaaaagtag ataaattttt tcatttttta tttttaatta aaattttatt gagaaactta
    11581 aataaaacca tttcgattat caattgtaaa attttcttta tctgaaattt attacctttt
    11641 ggttttcaat agcattaaaa attttactga acctaaaatt tctgctaatt tcgatcaccc
    11701 taaatattta taaacaccta aaattcagtt ctagtattta tttttttatt taaatgtaat
    11761 ttgtaattga ttaagtttat taaaaccaat ttatgataat gactcttacc agatccatac
    11821 tttttatatt ctctttttta attatcactt ctgatattta tgcacagaca ttcaattatc
    11881 ctatagtttt tgtttcaaga aatcatgaaa cgaacgggaa tatcttttat cctgatgcag
    11941 gacttttacc gggaatggga ccattttcaa gattttctgt ggtgggagga aagcttatgg
    12001 taagagataa acagggtgtc attactactt tacttgatag ttcaattact ctaaacggaa
    12061 tcagattaat agattttcag cagccctgtg taaactggag cggagataaa attctatttg
    12121 ctggcattga agatattgac agtagctgga gaatttatga gatttttagt gacggttcga
    12181 gaatgagaaa aattactttt acaaacagaa atattgatct gctgcagttt ggtcctgccg
    12241 gtcataaatt cagaacttat gaggacattg acccgattta tctacctgac ggcagtataa
    12301 tttttgcatc tacaagatat ccgacattat cggaaattgg tggttttcag acaactaatt
    12361 tatttattgc agattctctt ggaaataata tccgaagagt tacatctgaa aggaacggag
    12421 gcgaaaagcc tactatagat cctgtttccg gtagaatact ttattccaga tggtgggtga
    12481 atatagataa accaagtaat gttacaagtt caggtcttac aagaattgat tctcttgctt
    12541 tatctcagga cattgcaaac atatggcagg ttgatattgt aaatccggac ggagatgcac
    12601 tgaagctttt tgctactgat ccaaggaata gaaatagttt atcatcatac cgacccagag
    12661 ttgctgcaaa tggaaaatta ttgacagtat ttataccaag tttaccaatt gtaactactg
    12721 gaggaagtac cggaattcgt ttttatgaac agggattttc agaagcaaaa aaaataatcg
    12781 gcactgacag cacaactcca ttatatattc agaacccacc ttccaccggt acaatgcaac
    12841 ctccttacgc tactgatcct ttaggattac ctgacggaag tgttttgttt tcttatgctt
    12901 caactgtcga agctcaggat tatgggatct atacagttaa cataaatgga acaggactta
    12961 cgcaggtagt tgattttccg ggtacacttg agctgaatgc agagcttctg attccaagga
    13021 aagttccacc gaaagttttc tatctaaatg attttgatac taataatgtc cctcctactt
    13081 caaatccaaa ttctttttat caaggaggtt tgtttagatt tgactgcctg aatgtttatt
    13141 caaatgctcc ggtagattct ccaatcggtg atggtcctcc tattaagaag aatgccagat
    13201 tcagattttt tctgaatttt caaagagaaa atgagaatgg tcaagatctt ccaatactat
    13261 tcagagagat tgcgcttgac agggacggaa agatagctga aggtgatata ccggctaatg
    13321 tctcaatgtt tgaacagatc atagacagta caggaaatgt gcttcaaaac aaaaaaggtg
    13381 attttgctca tgtaacgggt atgaattttg ggaataacgg ttcgggtact aaatgtgtcg
    13441 gctgtcacgc aggtcataca atgattactg ttccaattaa tttaactgaa ggtacattca
    13501 caaatctgtc tacttctgct gatgtaaggg aaagcagttt taaaacaaat ggaaactttg
    13561 catataaagg tcagaacgtt gttgacagaa aagcaagaaa tcctgatcag tcagttaact
    13621 ggatctctaa tggtacaata aatgaatttg ttgttcttaa atgggaaatc cctatcgata
    13681 tcagaagaat aaaattatat gatatattac caaatcaaaa taataatacg gatatacatg
    13741 tgacagattg tgaaatattt ttttattatg agaatagtgt tgcaaatcat attcagtcca
    13801 caggtccttt aaatactgat ggtttggaat tgaatgtact gccgattaca acaattgaca
    13861 gtgcaaagat catcgtaaaa agttttacag gaaacatcaa caatattcaa tccgcaggtc
    13921 tggcagaagt agaagtaaat gcaagggtta gttctataga cctggtgaat atcagtaaca
    13981 atgaaattgc tttgtatgat tacaaacttg aacagaatta tccaaatcca tttaatcctt
    14041 caactataat aaatttcaca ttaccaaaag ctcagactat ttctcttgaa gtttttgata
    14101 ttacaggaag gaatattgct actctgataa atggacgtgt ggaaggcggg agaaattcaa
    14161 taacctttaa tgctgcaaat ctaccgtctg gaatttattt ttacagaatg acagcagcgg
    14221 gaaaatttgt agaagcaaaa cgaatgatcc ttctaaagta aaaaatcatt tttatcttat
    14281 gtttatttca aattgttaat attgaaattc atacaatctc aaaacaaaat ctgaaatgta
    14341 tggaaaacaa atccaacaaa aataatattg gtcaactaca aggaaagctt cttatttcag
    14401 ctcctttttt agatgatatt ttcaaaagag cggtaatatt gattacagag aataatgatg
    14461 aaggctcagt tggatttatt ttaaacaaaa ttactgaata caaacttcat gaaataattg
    14521 aggaatttcc tgagtttgat gcaaaggttt ttatcggagg acctgtacag caggaagcac
    14581 tgaattttat tcataaagcg ggcgacatcc ttgaaggcag ttttcaaatt agtgaaggag
    14641 ttttttgggg tggtaatttt gagcatttaa aaatcctaat tgaaaataat gcagttgatc
    14701 cggacgactt tagatttttt ctaggatacg caggatgggg taatggtcag cttgagaatg
    14761 aattaaagct aaaatcatgg tacgtaaatg attctttaaa cgataatatc tttagtcata
    14821 actcagcaaa tctctggagc aaagtattac gaagtatggg aaatgaatat actgtaattt
    14881 ctacttttcc agaagatcct tc
//
LOCUS       DAORVQ010000074        14897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1798_length_14897_cov_4.652810, whole genome shotgun sequence.
ACCESSION   DAORVQ010000074 DAORVQ010000000
VERSION     DAORVQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14897
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1798_length_14897_cov_4.652810"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLX80_07600"
     CDS             <1..180
                     /locus_tag="PLX80_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQY20686.1"
                     /translation="PQLTETFDKFSLSNKREYIEWLTEAKSETTRNKRLQTALEWISD
                     GKPRNWKYMKKYSGK"
     gene            450..1211
                     /locus_tag="PLX80_07605"
     CDS             450..1211
                     /locus_tag="PLX80_07605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQY20687.1"
                     /translation="MLLFNMLACLLACLSFIIPTQSYSQLTYPTRYPTAVIPHNADGN
                     RLFFLYMPMEYQDNAVQDTFKLYYLIGGTHDKRYLARSTDYGITWTDTQRVYTDSTYE
                     SIIMNPDSTRKYMAYNGWRGTDPKKSYMFSHYSDDGGFNFINKTQVMELGEDKSFIWN
                     NDTQEFWGYVRPRNIEKSCNCYIHNCYTLGNGVRKIALMKNSMRFPSSGDWSNRDTIL
                     FVESSDYSNSISPDYKTQTYYMQVFRSGNDWWGLV"
     gene            1246..2100
                     /locus_tag="PLX80_07610"
     CDS             1246..2100
                     /locus_tag="PLX80_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20688.1"
                     /translation="MILLIPEYTSDVELVWSDDGEDWFRTNNRQPIIALHDSINTIYS
                     VGTVVGDSLYTYSAESTILHAGYTTNGCGNTAKAESLFKGKYYSIYLYKISIDKLNEW
                     RPPSVFNINCTVEGFLNTGSGKHNLRDTLIAELRNSTSPYGIASTTQSIIDSVNFLGN
                     FNFPHVDSENYYLAIYGRNSFETWSANTFSISNGVENTYDFTTGYSKAYGGNLVLKSS
                     KYCIYSGDLNSDDLIDNDDQTIVDNDISLVATGYVVSDVNGDFVADISDGSIVENNVT
                     NFVATIRP"
     gene            2148..4148
                     /locus_tag="PLX80_07615"
     CDS             2148..4148
                     /locus_tag="PLX80_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20689.1"
                     /translation="MKNLNKILLIIALNLTFSFSVYSTPTNQFINSVSPPMNANSVVK
                     SSNIVIIFEQTMNGSTMTDANVKVFGYQTGLMNLSLDFNSVANTLTINPVNEFKNGEK
                     ISVTLTSGIKTISNQSITPFVFLFRAKALGGTGSFNLSSEISNTVSGYIRSGDIDIDG
                     DIDLVINNAVYINNGNALFNISQTLNQQGRPDIFDADNDNDLDIMISNIGTIYYYKND
                     GLGNFSFFSTFPGIVWSYCDLSGNGFMDITDFVNTSEVRTAFNFNGNFISNPVVTLFG
                     QCVNNGDLYDNLLIDDYNNDGNMDIMAISGWINGSSITFYDICRNYNLLEKNGIGEFI
                     SQDAFYHFSERWSPIIFEAADSKSFDFNDDGLVDIESPILKLNNNSNGLFTETGGFVV
                     FSSSLDFDFNGDGFIDLSANNSGSPLLCYLNDGSGDFSFYIGNNLQFASASASGDFDN
                     DGDIDIAIKEYSNDKVAILLNGDSPLPVELSSFTSSVNSNSVKLNWSTSSEQNNSGFE
                     IQRSIVKNETPDAWTKIGFTDGQGNSNTAASYTFEDKNLLSGKYKYRLKQIDFNGNFE
                     IYDLSNEVVIGIPTSTELMQNYPNPFNPVTNISYRLSENGFVTLKVFDNSGREVKTLI
                     NEFKEAGYYTNEFNGSDLASGIYFYKLTAGDFVQTKKLSLVK"
     gene            complement(4726..5085)
                     /locus_tag="PLX80_07620"
     CDS             complement(4726..5085)
                     /locus_tag="PLX80_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HQY20690.1"
                     /translation="MTNNVYWLLETNIKEGETENLKTLMTEMTESTKENETGALNYEW
                     SVSEDGKSCNIFERYSDSEAALSHMDSFGKNYAARFMSILEIKKFVVYGKPDEKVIGA
                     LTKIGAKIMKPLGGFQR"
     gene            5256..7019
                     /locus_tag="PLX80_07625"
     CDS             5256..7019
                     /locus_tag="PLX80_07625"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HQY20691.1"
                     /translation="MVILFQESYSSVNYATVIYTSPVNNAKLVNSFENIIFKTEKNLE
                     NERLVCIVKYFDGRLIDGNIKLASDKKTFIFDPVYPFKKGEQITADLISLKDNRLIKS
                     ISFTTSINILSESEKKKVISDIREKEFGQTQKQVYRIPENSNGFNTGGVIMPQITITK
                     NNNPSNGTLFFANIARDTLTSPFIMSMKNNGEFLFYREMPKTCYDFKVQNNGLITYFQ
                     NIAMKFYALDSAYTVVDSFATGNGYETDLHDILIKEDGNVLLMSYDVQVIDMSLVVSG
                     GQKDANVTGLIIQEIDSDKNVIFQWRSWDHFQITDADKENLSLGNVDYCHGNAFDVDY
                     DGNYVISSRNMSEITKINKETGAIIWRLGGKNNQFTFINDSIKFSYQHAIKRTAAGTF
                     TLFDNGNYHKPSFSRSVEYRIDETNMTAEKVWEYRNEPSIYTFAMGYTERLKNGNTSI
                     SWGFAPTAYTEVNSNNEIVYEFNFDSTNFSYRIFREDWNVSPDGIPNIPVDFAVTLNY
                     PNPFNAQTNIYFNVINNSNVTLKIYDILGKEVATLLNNEFKETGKYVSNFNSVALSSG
                     IYFYKLQIGNDFYTHSMALVK"
     gene            7119..7535
                     /locus_tag="PLX80_07630"
     CDS             7119..7535
                     /locus_tag="PLX80_07630"
                     /inference="COORDINATES: protein motif:HMM:NF028494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20692.1"
                     /translation="MKYLLIIFLGLLFFQSCGEKTAEKNLLSEEEKRKQQEEMEFSRK
                     VIGAEIMKGMYSYNSNTNHFTECNTNRKYILVGESETNEMKKAYQDKRFVSPSLLAFV
                     EIEAFQSYQDIDGNNIPDTVLVVTKFLNFDAEKKCE"
     gene            complement(7555..8328)
                     /locus_tag="PLX80_07635"
     CDS             complement(7555..8328)
                     /locus_tag="PLX80_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HQY20693.1"
                     /translation="MSDKELKKYLDSLYSKYHKKFSSKDPVWILHEFYDKKDIEIIGF
                     ITSCYTYGKVESINAFIRKLLNITGNKVHEFTSNYNEHKDKKYFEDMYYRFNSPEDLS
                     LLISKIRNVIIEYSSLEDLFLKYYSESHENILPALHAFTKELNEKKGNEESLKYLVPL
                     AENGSACKRLNLYLRWMVRNDYIDVGIWKNVSVSKLLIPVDTHVYKVSKILGLAERNT
                     CDMKFVIELTNKLKTFNAEDPVRYDFAICHAGMEGFLKS"
     gene            8352..9035
                     /locus_tag="PLX80_07640"
     CDS             8352..9035
                     /locus_tag="PLX80_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQY20694.1"
                     /translation="MEKVFLIGDNQFLMTELSEILESKYEIQDTLDETTEIIFELSNT
                     GKDIKCKILNFIDEKNSKATVISSSLCITVLEQAINSKYPERIIGASIYQSFSESKGM
                     EIAGCKFTEEKHLNKCHEIFEVIGKEVTVVTDRAGMVSMRIISMIINEAYLVLQEGTS
                     NREDIDTAMKLGMNYPHGPIDWSEKIGLDLIYNILFTMNEEFGDDRYRITPLLKEKYL
                     ETFKSKEQV"
     gene            complement(9049..9180)
                     /locus_tag="PLX80_07645"
     CDS             complement(9049..9180)
                     /locus_tag="PLX80_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20695.1"
                     /translation="MDKTQTKKPTSFLTKIVLFIIIAATIFSIYYTIKLVYSGFTFR"
     gene            complement(9227..10531)
                     /locus_tag="PLX80_07650"
     CDS             complement(9227..10531)
                     /locus_tag="PLX80_07650"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HQY20696.1"
                     /translation="MNPVEIIKLKRNGNALSRDQIKYLIEGYLDGNVTEYQMAAFLMA
                     VYFNNMNEDETFYLTEIMLNSGTVLDLSHIKNPKVDKHSTGGVGDKTSLILAPLVASC
                     GIYVPMISGRGLGHTGGTLDKLESITGFNVNYEIEDFKRILETVGLVMLGQTSELAPA
                     DKRIYALRDVTATVESIPLITSSIMSKKLAEGADALVFDVKIGVGANLPDKDESIQLA
                     KKLISTSKKFGKKAIAVLSDMEQPLGMKIGNWLEIEECIEMMNGKLIPDLYKLTNVLA
                     GAMLYLGDAAGSIEEGEKIALQKISDKSAYKKFLEMVEIQNGDVSYVKDWANLKRPEF
                     SKLIKAEQDGFISEMHASDFGYAAIELGCGRKKTDDKIDYLAGIILHKKCGNEIRKGD
                     VICELFSDDKLRIDIAEKRLEQSIKISKIKPKVNPLIIDILD"
     gene            10679..13105
                     /locus_tag="PLX80_07655"
     CDS             10679..13105
                     /locus_tag="PLX80_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQY20697.1"
                     /translation="MSKSPALKFILIYISGILCFDLFSQFTIVIRITFITFLLIVIFL
                     RFKDKFEPICRALTFILIFIMGLFNSQFDFNRSNQSVLKNYPDTQKNSYNVITGIVTE
                     IPVVNKSGVKLVIECRSISSRRDTLHLNGKVIATLRENIYSKSSEQIPVINPGDEISL
                     KGKLSTSPGRRNPGDFDYKKYLESRDIHKLFYSNGYKDVKIISQKNLSFIYQYIIFPA
                     KIFALNNIDTLLSGDNASYLKGLVTGERSDISDETKEAFINAGVMHLIAVSGLNVAYV
                     IIFVNILLSLLRVPFKFRIYITCLFLIFYCMFTGSPASILRATIMGITVLMSFVFERK
                     IYFYNFIGVSAMCLLIFDTRLLFDTGFILSYAATFSMVIIYNRFEKSFLHNLKSPKTK
                     FKKIVSWISVLFFTSLAAQIGTLPLTSLYFGKISFISLLANVVAVPLANISLAIGFLQ
                     ILTGIISFDLSSIIAETNNILLSIQLEFIKFCAGMKFSFIYIKNFTLSIIISYYIICI
                     LLIFAKTLHMFFRNLIISFLIILILLTNNVSYEQKMTVTFLDVGQGDCALIETPDGKN
                     ILVDCGNSTKTFDNGEKTIAPLLRRKGIDKIDMIILTHLHMDHIGGVNYLLKHFEIGS
                     IFESGQDHITDFTMVTDSLISINNVKRTIVRSGDMIDSFKDMRLYFLYPDKAHADASD
                     HPSYGNLNNGSVVFILKYRETEILFTGDAEAAGELYIKNHYEDFIDTDVLKAGHHGSI
                     TSSTIPFILKNSPRIAVISCGMNNKFNHPSKIILDRLKRSGAEIFRTDNEGAVIIETD
                     GYEIKATNKK"
     gene            complement(13106..14383)
                     /locus_tag="PLX80_07660"
     CDS             complement(13106..14383)
                     /locus_tag="PLX80_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20698.1"
                     /translation="MEKKYCSILILLLFSIYYFGFGLNTSHSQPLNKKVTDSDSLNHR
                     PVVTDSSKSELKQGGSVKIKIEEVLNENSNKNEGNLGDNILLKVSNIQDLLTESKIKG
                     KPIVLYIDGLPINNSAGRIIRDTSAKVWLKFPLKLKGSEESWLEILKRPKLASRSTNL
                     VTISAGIEGEKELESNRENFSLIVYNEMAYFQFIGISILLLLITFYLAIKTDMLREPV
                     TDNSSKKKRPYSLALTQMAFWSYLVVSTYLALYIITKEFPTITNSVLALMGISSATAL
                     GSMMINFKKIKLSNEKIISMNAEMRSIDDRVSLINELMKEDITPMRKEEFRSELESLN
                     KRQYEIHDLLKDEKTSHEYCCKGFLNDILSDSGGVSLYRFQIAVWTLVLGYIFIFTIW
                     NSLKMPEFDNTLLALMGISSGTYLGFKIPEQRQ"
     gene            complement(14435..>14897)
                     /locus_tag="PLX80_07665"
     CDS             complement(14435..>14897)
                     /locus_tag="PLX80_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20699.1"
                     /translation="KASVKFFKGNNLTGEFENVSYQNFESGAVMIQFSPDGNGYVINA
                     EDFGSQGNNNSYLVTENGKMDFPENTVSGSGKLINILNLMYTDNGKLFYTGITELDTT
                     DYSYTEEIFTDNISLGKHYKQVQKIKYNRSDNTISFLGSRKNKIYSVTVRF"
BASE COUNT         5040 a         2337 c         2431 g         5089 t
ORIGIN      
        1 cctcagttaa cagagacatt tgataaattc agcctgtcaa ataaaagaga gtatattgaa
       61 tggctgactg aagcaaagtc agaaactacc cgaaacaaaa gactgcaaac tgctttagaa
      121 tggatctctg atggaaagcc taggaactgg aagtatatga agaaatattc agggaaataa
      181 tttcaaattt caaatttcaa atttcaaatt tcaaatttca aattttaata tgttcatttt
      241 aaatattcat ttataattaa taattgtcct tttaacattc ccgcacactc tttgtcttta
      301 ccttggatac ggtgatctat tagaaaatgc ttgccgaaat attcaaaatt cgtaattcca
      361 aattgaacac agttccgtgt cttgacatta ataattattg tatttacttt ctaaaaaaat
      421 tagtaaagga gaaaatttga aaaaccaaat tgctactttt caatatgctt gcttgcttgc
      481 ttgcttgctt aagttttatc atacctacac aatcttacag tcagctgact tatcccacca
      541 gatatccaac tgcagtaatt cctcataatg cagatggtaa cagattattt ttcttatata
      601 tgccaatgga atatcaggat aatgccgtac aggatacttt taagttgtat tatcttatcg
      661 gaggaactca cgacaaaaga tatctcgcaa gatcaactga ctatggcata acatggacag
      721 atactcaaag agtttatacc gattctactt atgaatcaat aataatgaat ccggattcta
      781 caagaaaata tatggcttat aatggatgga gaggtacaga tccgaaaaag tcttatatgt
      841 tttcacatta ttctgatgac ggaggattta attttattaa taagactcaa gttatggaac
      901 ttggggagga taaatcattt atctggaata atgatactca agagttttgg ggatatgtta
      961 gaccaagaaa tattgaaaaa agctgtaatt gttatattca taattgttat actttaggaa
     1021 atggagttag aaaaattgct ttaatgaaaa acagtatgcg atttccaagt tccggtgatt
     1081 ggagtaacag agatactatt ttatttgttg aatcttcaga ttattcaaat tcaatttcac
     1141 cagactataa gacacagaca tattacatgc aggtatttag aagtggtaat gactggtggg
     1201 gattagtatg aatgtacaga gttgggaata atggtggaga aaataatgat actccttatc
     1261 ccggaatata cttctgatgt tgaattagtt tggtcggatg atggagaaga ttggttcaga
     1321 actaataata gacagccaat aattgcttta cacgactcta taaatacaat ctattctgtt
     1381 ggaacagtag tcggagattc attgtatact tattccgctg aaagtactat tcttcatgca
     1441 ggttatacaa caaatgggtg tggaaataca gcaaaagcag aatctttgtt taaaggaaaa
     1501 tattatagta tttatttgta taaaataagt attgacaaat taaatgaatg gagaccacct
     1561 tcagtattta atattaattg tactgtcgaa ggatttttaa ataccggatc aggaaaacat
     1621 aatttacgtg atactttaat tgcagagtta agaaattcta cttcaccata cggaatagct
     1681 tccacaacac aatctataat tgattcagta aatttccttg gaaactttaa ttttccacat
     1741 gtagattccg aaaattatta tttggcaatt tatggaagaa attcatttga gacttggagt
     1801 gcaaatactt tttcaatttc aaatggagtc gagaatactt atgatttcac taccggttat
     1861 tcaaaagcat atggaggaaa tttagtttta aaaagcagta aatactgcat ttattcaggt
     1921 gatttaaatt cagatgattt aattgataat gatgatcaaa ctattgtaga taatgatatt
     1981 tctttagttg caactggtta tgttgtatct gatgtaaatg gagatttcgt tgcagatata
     2041 tctgacggta gtattgtaga aaataatgtt actaattttg tagcaacaat ccggccataa
     2101 tttaacttat aataataaaa attttttaaa aaaataaatt aaagatcatg aaaaacttaa
     2161 ataaaatatt gttgattatt gcattaaatc tgactttttc atttagtgta tattctacac
     2221 caacaaacca attcataaat tccgtttctc caccgatgaa cgcaaacagt gtagtcaaat
     2281 cttcaaacat tgtaattata tttgaacaaa caatgaacgg ctcaacaatg acagatgcaa
     2341 atgtaaaagt attcggatat cagacaggac tgatgaactt gtccttggat tttaattctg
     2401 ttgcaaatac acttaccata aatccggtta acgaatttaa aaatggtgag aaaattagtg
     2461 taactttgac ttcgggtatt aaaactattt caaatcaaag cataacacct tttgtatttt
     2521 tattcagggc aaaagcatta ggaggaacag gcagttttaa tttatcatct gagataagca
     2581 ataccgtcag cggctatatt aggtcaggag atatagacat tgatggagat atagatttag
     2641 ttataaataa tgctgtttat ataaataacg ggaatgcgtt atttaatatt tctcaaactt
     2701 taaaccaaca gggaagacct gatatctttg atgccgataa tgacaatgac ctggatataa
     2761 tgattagcaa tatcggaacg atctattatt acaaaaatga cgggttggga aattttagtt
     2821 ttttctcaac atttccggga attgtctggt cttattgcga tctgtcagga aatgggttta
     2881 tggatataac tgacttcgta aatacaagtg aagttagaac agcatttaat tttaatggta
     2941 atttcatctc taatcctgta gtcacattat ttggtcaatg tgttaacaac ggtgatctct
     3001 atgataactt attaattgac gattataata atgacggaaa tatggatatc atggctatta
     3061 gcggatggat aaacggtagc agtataactt tttatgatat atgcaggaat tataatttat
     3121 tggagaaaaa cggaatagga gaatttattt ctcaagatgc tttttatcat tttagcgaaa
     3181 gatggagtcc gattatattt gaagcagcag actcgaaatc ttttgacttt aatgatgatg
     3241 gattagtaga tattgaatca ccaattttaa aactcaacaa taattcaaac ggattattca
     3301 ctgaaactgg tggatttgtt gtatttagta gttcattgga ttttgatttc aatggtgatg
     3361 gatttattga tctaagtgct aataatagtg gatcaccatt attatgttat ctaaatgatg
     3421 gatcaggaga tttttctttt tatataggga ataatctgca atttgcttcc gcatctgctt
     3481 ccggtgattt cgacaatgac ggagatattg atattgcaat taaagaatat agtaacgaca
     3541 aagttgcaat tctcctcaac ggcgattccc ccttacctgt cgagctttct tcatttactt
     3601 caagtgtaaa ttcaaactct gtaaaactaa actggtcaac ttcatccgaa caaaacaatt
     3661 ccggctttga aatccaaaga agtattgtga aaaatgaaac tccggatgca tggacgaaga
     3721 tcggattcac agatggtcag ggaaattcta acactgctgc gagttatact tttgaagata
     3781 aaaatttact gtcagggaaa tataaatacc gacttaaaca aattgacttc aatggtaatt
     3841 ttgaaattta tgatctgtcg aatgaagtcg tgatcggcat tcctacatcg actgagctga
     3901 tgcagaacta tccgaatccc tttaatcctg ttacaaatat ttcatacaga ctttcggaga
     3961 atggttttgt gacattaaaa gtttttgaca attcaggaag agaagtaaag actcttatca
     4021 atgaattcaa agaggcagga tattatacaa atgaattcaa cggcagtgat cttgcaagcg
     4081 ggatttattt ttataagctg accgcgggag attttgtaca gacaaagaag ctgtcgctgg
     4141 ttaagtaacg aattacaaat gttaaatcgt gcagcgagcg aagctatcta aatgtcaaat
     4201 attaaaatga gccattgctg gtcttagagg ctgaagcgag cgtgaccaat aacaaacgga
     4261 acctcgtaac ctgacattat aatttgatgg aaagctccgg actttgtcat tcctgcgaat
     4321 gcaggaatcc actgacctga aagtactatt caattctaaa gtaaaaatta atttctgtta
     4381 aatggtaagc aactttctct ttctttcaaa ttcatgtaca acattcatat caaaatgaat
     4441 ttagaaatgt aataccgttt cctcatacat ggattcctgc atgcgcatga atgacaaagt
     4501 tttgttagct tcgaactgtg aatctttgag tcttcgagcc tttgaagtaa accgaaaatt
     4561 gtaatttgat acttgattta ctttgtgtct tattgacttg gtggaaagaa ttaaaataaa
     4621 tcctcccaag caggggcttg ggaaggagta taaagtactt tgataattga taactgatta
     4681 ttgatttact ttgtgtctta ttgacttggt ggtaaagtaa ttgacttatc tctgaaatcc
     4741 accaagcggt ttcatgatct ttgcaccgat cttagttaaa gcacctatta ctttctcatc
     4801 aggcttacca tagactacaa attttttaat ctccagaatg ctcataaatc ttgctgcata
     4861 attttttcca aatgaatcca tatgagacaa tgccgcttcg gaatctgaat atctttcaaa
     4921 aatattacag cttttaccgt cttcacttac tgaccattca taattcaaag caccggtttc
     4981 attttctttt gtactttctg tcatttctgt catcagagtt ttaagatttt ctgtttcacc
     5041 ttcctttata tttgtttcga gtagccagta aacattgtta gtcataatta acctttctgt
     5101 tattttatta taattttgtt ttgaatatat tgcattatta attttgaaac tttttaaaaa
     5161 actgtttaag ataactaaaa tcagttttaa taaataaata attttttata ataaatctat
     5221 cagtgttgtt aaaatattac atttcaataa ttttattggt cattttattt caggaatcat
     5281 attcatcagt taattatgcc acagtaattt atacttctcc cgttaataat gcaaagcttg
     5341 taaacagttt tgaaaatatt attttcaaaa cagaaaaaaa tttagaaaac gaaagactgg
     5401 tttgtattgt aaaatatttt gacggtagat taattgacgg aaatataaaa ttagcctctg
     5461 acaaaaagac ttttatattc gatccggttt atccattcaa aaaaggagag caaattactg
     5521 cagatctgat ctctttaaaa gataacagat taataaaaag tataagtttt acaacttcca
     5581 taaatattct aagtgaatcg gaaaagaaaa aagtaatttc agatatccgc gaaaaagaat
     5641 ttggacaaac ccaaaaacaa gtttacagaa tacctgagaa ttctaatgga tttaatacag
     5701 gcggggttat tatgcctcag atcacaatta caaaaaacaa taatccatca aacggtactt
     5761 tattctttgc aaatattgca cgtgatactc ttacatctcc tttcataatg agtatgaaaa
     5821 ataatggtga atttcttttt tacagagaaa tgccaaagac atgttatgat tttaaagttc
     5881 agaacaatgg actgatcact tattttcaga atattgctat gaaattctat gcgcttgatt
     5941 ctgcttacac tgttgttgac agcttcgcaa ccggtaacgg atacgagaca gacctccatg
     6001 atattctcat aaaagaagat ggaaatgttt tgttgatgag ttatgacgtt caggtgatag
     6061 atatgagtct tgttgtctct ggaggtcaaa aggatgcaaa tgtaaccggt cttatcattc
     6121 aggaaattga ttctgacaaa aatgtgatct ttcaatggag gagctgggat cattttcaga
     6181 tcacggatgc agacaaggaa aatctttctc tcggcaatgt tgattattgt cacgggaatg
     6241 cttttgatgt ggactatgat ggaaattatg taatttccag caggaatatg tcagagataa
     6301 ctaaaattaa taaagagacg ggagcaataa tctggaggct cggaggaaaa aataatcagt
     6361 ttacttttat taacgattct atcaaatttt cctatcagca tgctataaaa agaacagctg
     6421 ccggtacttt tacattattt gataatggaa attatcataa acctagtttt tcaagatcag
     6481 tcgaatatag aattgatgaa actaatatga cagcagaaaa agtatgggaa tacagaaatg
     6541 aaccttccat ttatactttt gcaatgggtt atactgaaag acttaaaaat ggtaatactt
     6601 ctatttcctg gggatttgct ccgactgctt atacagaggt aaacagtaac aatgagatag
     6661 tttatgaatt caattttgat tcgacaaatt tttcttacag gatattcaga gaagactgga
     6721 atgtatcccc tgatggaatc ccaaatatcc ctgtggattt tgctgtcaca ttaaattatc
     6781 caaatccttt taatgctcag acaaatattt attttaatgt tataaataat tctaacgtta
     6841 ctctgaagat ctatgacatt ctcggaaaag aagtcgctac attattaaat aatgaattta
     6901 aagaaacagg aaagtatgtt tcaaatttta actcagtggc tttatcttca ggaatttatt
     6961 tttacaaact acagatcgga aatgattttt atactcattc tatggcactt gtaaaataaa
     7021 atacaaatta caaatttcaa attacgaatt aataaattca gttagtttgt attttaacaa
     7081 acaaaaattt ttcagcaaaa ttcttttaat gtattaatgt gaaatactta cttataattt
     7141 tcttaggatt actttttttt caaagctgtg gtgaaaaaac tgcagagaaa aatttacttt
     7201 cggaagaaga aaagaggaag caacaagagg aaatggagtt tagccgcaaa gtgataggtg
     7261 ctgagataat gaaaggaatg tatagttata acagcaacac aaatcatttt actgaatgta
     7321 atacaaaccg aaaatatata ttagttggtg aaagtgaaac caatgaaatg aaaaaagctt
     7381 atcaggataa aagatttgtt tcgccttccc tcttagcttt tgtagagatt gaagcatttc
     7441 agtcttatca ggatattgat ggaaataata ttcccgacac tgttttggta gtaacaaaat
     7501 ttttaaattt tgatgctgaa aaaaaatgtg aatgaatatt ataaaattaa agatttagct
     7561 ttttaaaaag ccttccatgc ctgcatgaca tattgcaaag tcgtatctga caggatcttc
     7621 agcgttaaat gttttaagct tatttgtaag ttcaattaca aatttcatgt cacatgtatt
     7681 tctttctgca agaccgagaa tttttgatac tttataaaca tgagtatcga caggaattaa
     7741 aagttttgag acagacacat ttttccaaat acccacatct atataatcat ttctcaccat
     7801 ccatctcaga taaagattaa gtctcttgca ggcagaaccg ttttcagcaa gcgggacaag
     7861 atatttcaac gattcttcat ttcctttttt ttcatttaac tcttttgtaa atgcatgtaa
     7921 cgccggcaga atgttttcat gtgactctga ataatattta agaaaaagat cttccagtga
     7981 gctgtattct atgatgacat tcctgatctt agatatcaat aaagaaagat cttcaggaga
     8041 attaaatcta taatacatgt cttcgaaata ttttttgtct ttatgttcat tataattaga
     8101 agtaaattca tgaactttat ttcctgttat attcaataat tttctgataa atgcatttat
     8161 actttctact tttccgtaag tatagcacga agtaataaat ccgattatct caatgtcttt
     8221 tttgtcataa aattcatgta gaatccagac aggatctttt gaagaaaatt ttttatgata
     8281 ctttgaataa agtgaatcta aatatttttt taattcttta tctgacaatt ttttaatata
     8341 cagttaaata gatggaaaaa gtttttttaa taggtgacaa tcaatttctg atgacagaat
     8401 tatcagaaat actcgaatcg aaatatgaaa tacaggatac gcttgatgaa accactgaaa
     8461 ttatttttga gctttcaaat acaggcaaag atattaagtg caagatcctg aattttattg
     8521 atgaaaaaaa ttcaaaagcc actgtaattt cttcttcact ttgtattaca gttctcgagc
     8581 aggcaataaa ttcaaagtat cctgaaagaa ttatcggtgc atcaatttac cagtcatttt
     8641 cagaatcaaa aggaatggaa attgccggat gcaaatttac agaagaaaag catttgaata
     8701 aatgccatga gatttttgaa gttatcggta aagaagtaac agtagtcact gacagagcgg
     8761 gcatggtaag catgagaatt atttcaatga tcataaatga agcttatctc gtcttgcagg
     8821 aagggacttc aaacagggaa gatatcgata ctgcaatgaa gctcggcatg aattatccgc
     8881 atggaccaat tgactggagc gaaaagatcg gtctggatct gatatacaat attctgttta
     8941 caatgaatga agaatttgga gatgacaggt acaggatcac tcccctgcta aaggaaaaat
     9001 atcttgagac ctttaaatca aaagaacaag tttaattttc ttaatttctt atctgaatgt
     9061 aaagcctgaa tatacaagct ttattgtata ataaattgaa aaaatggtag cggctataat
     9121 tataaacagt acaatttttg taagaaagct tgttggtttt ttagtctgag ttttatccat
     9181 ctttttaatt taaaggatat aaattttttt aatcaatcaa aatctatcaa tctaatatat
     9241 caatgatcag agggtttacc tttggtttaa tttttgaaat tttaattgat tgttctaacc
     9301 ttttttcagc gatatcaatt cttaacttat catccgaaaa taattcacag atcacatcac
     9361 ctttcctgat ttcgtttccg cattttttat gaagtattat accggcaaga taatctattt
     9421 tatcatcagt cttttttctg ccgcatccga gctcaatagc agcataccca aaatccgaag
     9481 catgcatttc agaaataaaa ccgtcctgtt cagctttgat caatttcgaa aactcaggtc
     9541 tttttagatt tgcccagtcc ttcacgtaag agacatcacc gttttgaatc tctaccatct
     9601 caagaaattt tttatatgct gatttatcgg agattttttg cagtgctatc ttttcacctt
     9661 cttcaatact tcccgctgca tcaccaagat agagcattgc accggcaaga acatttgtca
     9721 acttatacag atcaggaatc agttttccgt tcatcatttc aatacactct tctatctcaa
     9781 gccagttacc gatcttcata ccaagaggct gttccatatc tgataataca gcaattgctt
     9841 ttttgccaaa cttttttgaa gtagaaatca gttttttagc aagctgtatg gattcatctt
     9901 tatcgggtag atttgcaccg actccgatct tcacatcaaa gacaagtgcg tctgcacctt
     9961 cggcaagttt tttgctcatt atactggaag tgataagcgg gatactttcg actgtagctg
    10021 tcacatctct gagcgcataa attcttttat cggctggagc aagttctgaa gtttgaccaa
    10081 gcattacaag acctaccgtt tctaatattc tcttaaaatc ttcaatttca taattgacat
    10141 taaatcctgt gatggattct agtttgtcaa gcgtaccacc tgtatgccca agacctctgc
    10201 cggagatcat tggaacgtat attccacagg atgcgacaag cggcgcgaga attaaagaag
    10261 tcttatcccc tactccgccg gttgaatgtt tgtcaacttt tggatttttt atatgactaa
    10321 gatccaatac tgttccgctg ttaagcatga tctctgtcag ataaaaagtt tcatcctcat
    10381 tcatattgtt aaaatatact gccatcagaa atgctgccat ctgatactcg gttacattgc
    10441 cgtcaagata tccttcgatc agatatttta tctgatctct gcttagtgcg ttaccgtttc
    10501 ttttaagttt tattatttct accggattca ttgtgcaagt taaaaaaata ctttcgatat
    10561 ttaaagtctc atattatctg tctttcacaa agtgcttaaa ttgcaatttg cttttttcat
    10621 catttgtaaa taatttgaac tgacaattct ggaagaatta aatatctata tgaacataat
    10681 gtcaaaaagt ccagccttaa aatttattct aatttacata tcgggaattc tatgttttga
    10741 tctattcagt caattcacaa tagttataag aattactttt attacatttt tgctgattgt
    10801 aatttttcta aggtttaaag ataaattcga accaatttgc agagcactca ctttcattct
    10861 aatttttata atgggtttat ttaattcaca atttgatttt aaccgaagta atcaaagtgt
    10921 gttaaaaaat tatcccgata ctcaaaaaaa ttcttacaat gtaattacag gaatagtcac
    10981 tgaaattcct gtagtaaata aatcaggagt taaattagtc attgagtgta ggagtatatc
    11041 atccagaaga gatactttac atttaaacgg aaaggtcatt gctactttaa gggaaaatat
    11101 ttattcgaaa tcatccgaac aaattccagt tataaatccg ggtgatgaaa tttcactcaa
    11161 aggaaaactt tcaacttcgc cgggcagaag gaatcccgga gactttgatt ataagaagta
    11221 tcttgagtca cgagatatac ataaactttt ttattctaat ggttataagg atgtgaaaat
    11281 aatttcacag aaaaatctga gtttcattta tcagtatatt atttttccag caaagatctt
    11341 tgcattaaat aatatagata ctctgctgag cggagacaat gcttcgtatc ttaaaggact
    11401 tgtgacaggt gaaagatcgg acatttctga cgaaacaaag gaagctttta tcaatgccgg
    11461 agtgatgcac ttaattgcag tgtcaggact taatgttgcc tatgtaatta tttttgtcaa
    11521 tattttactt tcacttttac gcgtaccatt taaattcagg atatatatta catgtctgtt
    11581 tctgattttt tactgtatgt tcacaggcag tcctgcatcg attttacggg cgaccattat
    11641 gggaataact gtgctgatgt catttgtctt cgaaagaaag atatactttt ataattttat
    11701 tggagtatca gcaatgtgtt tactgatctt cgatacaaga ttattattcg ataccggatt
    11761 tattttatct tatgctgcga ctttttcaat ggtgataatt tacaaccggt ttgagaaaag
    11821 ttttttacat aatcttaaat ctccaaaaac caaattcaaa aagattgtta gctggatatc
    11881 agtattattt ttcacatctc ttgcagctca gatcggtact cttccactta catcacttta
    11941 tttcggaaag atttcattta tttctctatt agcaaacgtt gtcgcagtac cgcttgctaa
    12001 catttcactt gcaataggat ttttacaaat actgaccgga attatttctt ttgatctgtc
    12061 atcaataata gcggaaacaa ataatatttt gctgagcatt cagcttgagt ttattaaatt
    12121 ttgtgcgggt atgaaatttt catttattta tataaaaaat ttcactcttt caattataat
    12181 ttcatattac atcatctgca ttcttctaat atttgcaaaa actcttcaca tgttttttag
    12241 aaacctgatc atttcatttc ttataatatt aatattactt acaaacaatg tatcgtatga
    12301 acaaaagatg acagtaactt ttctagatgt cggtcagggt gactgtgctc tgatcgaaac
    12361 cccggacggg aaaaatatac ttgttgactg cggtaacagt actaaaactt tcgacaatgg
    12421 agaaaagaca attgcaccat tactgagacg aaaaggaatt gacaaaattg acatgatcat
    12481 acttactcat ttacatatgg atcatattgg cggtgtgaat tatcttctaa aacattttga
    12541 gatagggagc atttttgaaa gcggacagga tcacattact gatttcacaa tggtaacaga
    12601 cagtctgatc tctataaata atgtgaagag aacgattgta cgaagcggag atatgataga
    12661 tagttttaag gatatgcgat tatatttttt gtatcctgat aaagcacatg ctgacgcaag
    12721 tgatcatcct tcttatggaa atctgaataa cggttcagta gtatttattt taaaatatcg
    12781 tgagacagaa attcttttta ccggtgatgc agaagcagca ggagaattgt atatcaaaaa
    12841 tcattatgaa gattttattg atacagatgt tttgaaagcc ggacatcacg gtagtattac
    12901 ctcaagtacg attccgttta ttttgaaaaa ctctccgaga attgcagtaa tttcgtgcgg
    12961 tatgaataat aaatttaatc atccgtctaa gatcatactt gaccgattaa aaagatcagg
    13021 agcagaaatt ttcagaacag ataatgaagg tgctgttata attgaaactg acggatatga
    13081 aattaaggca acaaataaaa aataattatt gtctttgctc agggatcttg aaaccaagat
    13141 aagtacctga acttattccc atcagagcca gcaaagtatt atcaaactca ggcatcttca
    13201 gactattcca gattgtaaaa ataaaaatat atccgagtac taatgtccaa actgcgatct
    13261 gaaatctgta cagacttact ccacctgaat ccgaaagaat atcatttaaa aaacctttac
    13321 agcaatactc atgtgaagtt ttctcatctt tcaataaatc atgtatttca tattgtctct
    13381 tattcaaaga ttccagttca cttctaaatt cctctttcct cattggagta atatcttctt
    13441 tcataagttc atttattaat gatactctgt catcaataga tctcatttcg gcgttcattg
    13501 aaataatttt ctcatttgat aatttgatct ttttaaaatt tatcatcatc gatcccaaag
    13561 cagtggcaga gctgataccc attaatgcaa gtacagaatt tgtaatagtg ggaaactcct
    13621 ttgtaataat atatagagca agataagttg aaactaccag ataagaccag aatgccatct
    13681 gcgtaagggc aagactgtaa ggtctttttt tctttgacga attatcagtt accggttctc
    13741 tgagcatatc cgtttttatt gcaagataaa atgttatcag aagtaaaaga attgaaatac
    13801 caataaactg gaaatatgcc atttcattat aaacgatcag agaaaagttt tctcggtttg
    13861 attcaagttc tttttctcct tcaattcctg cactgatagt gacaagatta gtagatcttg
    13921 aagcaagttt aggtctcttt aatatttcaa gccaggactc ttcagatcct ttcagtttca
    13981 aaggaaactt taaccagact tttgctgagg tatcacgaat gatccttcct gcagaattat
    14041 ttattggcag accgtcaata tagaggacaa ttggtttacc tttaatttta ctttcagtca
    14101 aaaggtcttg aatattactt actttaagaa ggatattatc acccaggtta ccttcattct
    14161 tattcgaatt ttcatttaaa acttcttcga ttttaatttt tacagatcca ccctgcttca
    14221 attcagattt cgagctatcc gttacaacag gcctatgatt caatgaatct gagtctgtaa
    14281 cttttttatt cagaggctga gaatgcgaag tatttaatcc aaagccaaag taataaattg
    14341 aaaagagcag taaaattaaa atagagcaat attttttttc catgatagtt taaattttat
    14401 ttaataaact tttggaaact tctatttcat cgaattaaaa tcttaccgtg acagaataga
    14461 tcttattctt tctcgatcct aaaaagctaa tcgtgttatc ggatctgtta tacttaattt
    14521 tttgtacctg tttataatgt ttaccaagtg aaatgttgtc agtaaagatt tcctcagtat
    14581 aggaataatc agttgtatcc aattcagtaa tgccggtata aaataatttc ccattatcag
    14641 tgtacattag attaagaata tttattaatt tgccggaacc tgaaactgta ttttcaggga
    14701 aatccatctt tccattttcg gtaaccagat agctattgtt atttccctga ctgccgaaat
    14761 cttcagcatt gatcacatag ccatttccat ctggagaaaa ttgaatcata acagcaccac
    14821 tttcaaaatt ctgataagat acattttcaa attcaccggt taaattattt cctttaaaaa
    14881 atttcacaga tgctttt
//
LOCUS       DAORVQ010000075        14744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1822_length_14744_cov_5.940364, whole genome shotgun sequence.
ACCESSION   DAORVQ010000075 DAORVQ010000000
VERSION     DAORVQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14744
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1822_length_14744_cov_5.940364"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLX80_07670"
     CDS             complement(<1..1379)
                     /locus_tag="PLX80_07670"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20700.1"
                     /translation="MKKSYMQFFILCILVLFFSKIIFAQVNTEKETSDNKDSTEYKTE
                     TIDVDALKGFKRLTPITFENIKRAEIEKRYSVQDIPMLLNGYTSINSYSESGSSVGYS
                     YLTLRGFDQRRLSIMINGIPQNDPEDHQVYWVDISDLAASVESIQIQRGIGSALYGSS
                     AIGGSINVETIDFFKRKFLNLTAGLGDFNTSKYTFEYSSGNVENGFGFYGKFTKLYTD
                     GYRDLSWSDHLSYFLSAGKVFGNNSVLKVNLFGSPIQNHMAYLGVDYAALNGEITGDI
                     YNDRKINYLTYPEETDNYFQPHYEIVYNLQANKNLYITNTLSYINGNGYFNSLFPVDY
                     GYDFSYYRLDPFYVSDTTTFNSSYYRRNDDGTFYFDSTKGYEIVRSDMVTKLTVDNNT
                     FGWFPKVQMTHSQGKGNLVMGGELRFHNSEHYGEVVFGEALPQGTEPNHQYYYYNGGK
                     NTYSIFANEV"
     regulatory      complement(1440..1541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1729..3609
                     /locus_tag="PLX80_07675"
     CDS             1729..3609
                     /locus_tag="PLX80_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20701.1"
                     /translation="MQTIKIIIIAILILAAENSFPQTGVETIYRDTIIGIRQFNSFEL
                     LNSPDKRIYLQEDAEYNNNFFGGIYYQLVKNSNSLIIPFNNALNPRFTSFCNFPKIYN
                     VPIESFTVSNLSDQILILNSRISCDTISNDTTLLSTNSGISFRSIDIKNCTGFGISNN
                     DSVIFTCGTYDLINYAFQKSTNKGQTWIITDTLQDIKKLSDGGFIKCNPFDKNIIYIS
                     GKNNLLISTNSGSNFMNTAAPSFDKIFFNNNDHSLYGINSAYLFKSTDNGLSWQIIYV
                     SPSIEFTDLQISEENPNIIYAGTDNGIFISTNAGVDWSLYNNAFPGSNKILGLSKDIG
                     SNSPLYACTSDGLYKVTHAYYQSNNFITHFKTGNLFQYFVNNLPGKYYYTVTVEKDTV
                     FSNGLKYWKLITNNTASSATYEYIDSISLKWLKYEPSCINSDHYGNSLIIDFNMDTGS
                     IFKICNWNNVVTSKTISNNFLGDPTFLRNSTSYPYPFSFDVNREKYVEKFGYLGSSGF
                     GYSVTLTGAVIEGITYGVIINIENNQSTEIPGNFSLSQNYPNPFNPNTIIKFHCPKSG
                     HVSLKIYDVLGNEVEVLASENVSAGSYSVEWNAENYSSGIYFYRFTAGSFTDVKKMTL
                     LK"
     gene            3943..4362
                     /locus_tag="PLX80_07680"
     CDS             3943..4362
                     /locus_tag="PLX80_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20702.1"
                     /translation="MITRNIYAALFILFILNISSCSEENSLSVKNQISSADKIEFLYK
                     DGFDSTGKMIVKTSNITNQKDIIEIINTVSDEPFPYQYCISTGSMTFYKQDSVLISLV
                     YNTTDDYRHIAFNQYNKLTAVKLSEENARLLELYSAK"
     gene            4390..5646
                     /locus_tag="PLX80_07685"
     CDS             4390..5646
                     /locus_tag="PLX80_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HQY20703.1"
                     /translation="MKKLFVIWILILSVSANAQITPEIYSWQQNINNITGYNGIPADV
                     QQVFYSDNYSYIKCTGIPSYTIGPWAMNPNIPTNQNWTFKITRFPVPDPTPSTAGNGQ
                     IGVLKNGVVLFNYGDAMSYNNLNIWHRVAQYFEAVSFDSSGGHPAPGGVYHYHINMKY
                     LYTPVPSEHSPILGYMFDGYPLYGPYGFSDTNGTGSIRRMVSGYVKRNITTRTTLPDG
                     TVLPQNQWGPNISTQYPLGCFKEDYELNTNGADLDSHNGRFCITPEYPNGIYAYFITI
                     DSLGEPVYPYIIGNTFYGEVVPGNVPPGGHVTITEPVTQYTPLSISNNNASNNNAESF
                     NLYQNFPNPFNPETNIKFDILKTGYTTLKIYDTNGKETDILLNENLTPGSYIVKFNAA
                     DNPSGIYYYKLSSGKSVSVKRMMLIK"
     gene            5748..6095
                     /locus_tag="PLX80_07690"
     CDS             5748..6095
                     /locus_tag="PLX80_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY20704.1"
                     /translation="MPEKISRIILFVNDVLKCTEFYKNILALKVIGIANPDWTELKAG
                     NCCIALHKTGQKGKGDFHNNVKIVFYKKDVKKAKELIEKNGIKMGKIFTFEGMNYCDG
                     SDPEGNKFQFSDR"
     gene            complement(6111..7190)
                     /locus_tag="PLX80_07695"
     CDS             complement(6111..7190)
                     /locus_tag="PLX80_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY20705.1"
                     /translation="MDQKFKSINFITDGIGFSPEEYIEKLSEINRSEKIERDLYGNGG
                     STKLLEEEFAVITGKEKCIYLPTGTMANHLSVKLLNGENTKVIVPENSHMFRDEADGL
                     FSVHRKRLIPIGKGKPYFELDDLKETLDYLKSGETFDSGTGTVVIENPVRRADGKYVP
                     IETIKEISDFCRENGYKLHLDGARIHLASAYTNIPISEYSSFFDTIYISLYKCLNAAG
                     GAVVCGDSEVIDRIPHQIKIHGGTVFQSWNNTAMALHFQKNIESKWQEIISLSENLIV
                     EINKIDGIRIEKIEQGTNIFNLVPNQNIDFNEFSNFLGKEHKIYIGRITDTGKIKFTI
                     NESLFRMEINKILNIWEKGIEISKK"
     gene            complement(7192..8220)
                     /locus_tag="PLX80_07700"
     CDS             complement(7192..8220)
                     /locus_tag="PLX80_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016818960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY20706.1"
                     /translation="MISFKYELEKKEPKILPGGITRGASVVDFPASVGIAGVSMRLES
                     GGMRELHWHANAAEWGYVIKGSCRTTILHPDGTSFIDTFDPGDVWYFPRGYGHSIQGI
                     SDEECHFILIFDNGDFSEDHTFSITDFIASTPPEIVAQNLGITLDEVGMLPKREVYFA
                     KGKVPDDQSFTSASVSDPELTTKHRYPLLAQEPRRVPGGGTQKTVTQKEFPISKTMAG
                     SVLEIQPGGLREMHWHPNADEWQYYIQGKAQMGVFLAQGNKVIEDFNEGDIGYVPMGA
                     GHYIKNTGDEVLIVLVGFNDGDYLALDLSDWLRSNPDSLLQTNFEVGEDLIKKFPKEN
                     KFIIPSNK"
     gene            complement(8370..9284)
                     /gene="moaCB"
                     /locus_tag="PLX80_07705"
     CDS             complement(8370..9284)
                     /gene="moaCB"
                     /locus_tag="PLX80_07705"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HQY20707.1"
                     /translation="MVDITGKINTLRYAKAQASVKVSSQSTIDAINNKLVAKGDVFEM
                     SKAAALLAVKKTSDVIPDCHPLPIEFAAVNFSIEGLEIKIEMEVKTIYKTGVEVEAMH
                     GVSIAALTAYDMLKPIDKNIEISSIKLIEKKGGKTDFPEEFRKALNAAVIVCSDSISS
                     EKKEDRAGKVIISKLESLQINIFEYTIIPDEAADIKCKVLKLSDEKVNMIIFTGGTGL
                     SPRDITPEAIEPLLDRKIPGIEEAMRSYGQQRTPYSMLSRSVSGLIGDTLVLALPGST
                     KGAKESMDALFPFVLHIFEVMKAMKHKD"
     gene            complement(9463..9906)
                     /locus_tag="PLX80_07710"
     CDS             complement(9463..9906)
                     /locus_tag="PLX80_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQY20708.1"
                     /translation="MDKKKSKKLFLEGAIRPEFIADSISKHSTKKNIGAHQIFLGQIR
                     SDEIDSRIVQAIEFSAYNEMAEEEYFNIREIIFAKYDLICMHVYHSLGKVFAGEINMF
                     VFVSSVHRKDATEACREMVELIKTKLPVWGKEIFAGDEYSWKVNS"
     gene            complement(9909..10487)
                     /locus_tag="PLX80_07715"
     CDS             complement(9909..10487)
                     /locus_tag="PLX80_07715"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HQY20709.1"
                     /translation="MIKNNVTVAILAGGKSSRMGEDKGLMYLNDKMLIEYVIDEVKKI
                     SDKIIIITNNPEYEQFGYSCQSDLIKNAGPLGGILTSLTFSQTRKTLILSCDSPFITS
                     DVLSILIDKANGEDVLVFENNGEAEPLFAVYDKNCLEQIKELIKNNDLKMKNALNSLK
                     TVLYKFDEYDFNNKNFFTNINTPIELSKHQKI"
     gene            complement(10484..11689)
                     /locus_tag="PLX80_07720"
     CDS             complement(10484..11689)
                     /locus_tag="PLX80_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQY20710.1"
                     /translation="MISVKDAKNKLYKSVKKMPATIKNISDITGYFLSESIKSPVDLP
                     PFDQSAMDGFAVNFDDTVGNMNNEFSFLIIGEIKAGDKPIKFLKKNTAVYIYTGAAVP
                     INSTCVVMQEKAVVKKGSVTISLSDLKTVSNIRSKGSEIKKGQVALTKGHQINPASIG
                     FLCSMGIDKIKVIDKPILSVLSTGNELQRPGKKLSPGKIYESNSLMLESVIKECGYEI
                     SKINSVKDVKDKISFCINNMIKTSDVILISGGISVGKYDLVKEILHNLGVREIFYKVL
                     QKPGKPLYAGKYKDKLVFALPGNPAASLTCFYEYVLPCLNKMSGHSEFELNKKFLPLS
                     KPYELKGEKDLFLKAKIENDMITILEGQGSNILRSFTESNAIVFLNSGNKLLKKGSYV
                     ETHILPKSL"
     gene            11953..12360
                     /locus_tag="PLX80_07725"
     CDS             11953..12360
                     /locus_tag="PLX80_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY20711.1"
                     /translation="MDRINLEKDIRVFYVQAESFPDGIQTAFKKLQILIDTGKDRRCF
                     GISRPENNEIIYRAAAEELFEGEAEKLNCRTLVIKKGNYNFITVKDFMNNIQEIGKSF
                     EGLCSLSNTDPDGYCVEWYFNDTDVMCMVRSTD"
     gene            12389..13633
                     /locus_tag="PLX80_07730"
     CDS             12389..13633
                     /locus_tag="PLX80_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQY20712.1"
                     /translation="MKIKIALLFFTAALILISDVFSQQDSSDFKFITHKNKKDSTLLP
                     FYTEQGDWFRNPEIPSFVFQGPDKKFSLGIGGFFNYTASYDFDGISDNLDFVTYDIIV
                     PNDTIQKSQFQMYANTSLLYFKVITKTSHGNLVGYVSANFRGDGNNFNLYQMYIRYIG
                     FDLGVDWSTFSDAASWPVTINYVGLNSMSDALSTLIRYSYKFANGWKFTLGVEQPEFN
                     TAFTNTGSLKQTIPDIPAYLQYSFGNSHVRLSGLYRNLFYRDNISNADRTVTGTGISM
                     TGNVEITKKAQIFFQGLYGKGIGKYVQDLSIDDLSVTPVSAHAGELTALPVYAYYGAL
                     QYNFNSKFFTTIMYSQLKVQPDNFYSPSSYSFARQFTGNFFWQFLPAANVGLEYNFGD
                     RTNQNGQNSIANRVEFMMQYNF"
     gene            13768..14595
                     /locus_tag="PLX80_07735"
     CDS             13768..14595
                     /locus_tag="PLX80_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HQY20713.1"
                     /translation="MHEKNHKHDIAPWRHKLHEVIFEADTPTGKWFDIILIISIILSV
                     VVVMLDSIQVVKIENAELLFAAEWFFTILFTIEYILRLLSVSRPLHYAKSFFGIIDLL
                     AILPTYISLFYPGGQYLVVIRLLRILRIFRVLKLVQYVGESKILIQALKGSRRKITIF
                     IFTVLTIVTILGSLMYLIEGEENGFTSIPQSIYWAIVTLTTVGYGDITPQTVPGQFLA
                     VIIMILGYGIIAVPTGIMTVEMNKAFKQKGGIRACINCGADNHDEDAVYCKYCGKSL"
BASE COUNT         4788 a         2569 c         2218 g         5169 t
ORIGIN      
        1 acttcatttg caaaaattga gtaagtattc tttccgccat tataataata atattgatga
       61 ttcggttctg taccctgagg aagtgcttca ccaaaaacca cttctccgta atgctctgaa
      121 ttgtgaaatc tcagctcacc tcccattacc agatttcctt ttccctgact gtgtgtcatc
      181 tgaactttcg gaaaccaccc gaatgtatta ttgtcaactg taagctttgt caccatatca
      241 cttcttacaa tttcatatcc ctttgtcgaa tcaaaataaa atgtcccgtc atcatttctc
      301 ctgtaatatg aagaattaaa tgtagtcgta tcagaaacat aaaatggatc cagtctgtaa
      361 taagaaaaat catagccgta atctacagga aacagtgagt tgaaatatcc attgccattt
      421 atataactaa gtgtgttcgt tatgtataaa tttttatttg cctgaaggtt atatacaatc
      481 tcataatggg gctgaaaata attatccgtc tcttccggat aggtcagata atttattttt
      541 ctgtcattat aaatatctcc cgtgatctcc ccgtttagtg ccgcatagtc aactcctaag
      601 tatgccatat gattctgaat cggactacca aacagattca ccttcagcac agaattattt
      661 ccaaacactt ttcctgcact taaaaaatat gagagatggt ctgaccatga tagatctctg
      721 taaccgtctg tatataattt tgtgaacttt ccgtaaaacc caaatccgtt ttctacattc
      781 ccggatgaat attcaaaagt atatttactt gtattaaaat ctcccaatcc tgctgtcaga
      841 ttcagaaatt tcctcttaaa aaaatctatg gtctctacat ttatagatcc accgattgct
      901 gacgatccgt ataaagctga tccgatacca cgctgtatct gaatactttc aactgatgca
      961 gctagatcag aaatatccac ccaatatacc tgatgatctt caggatcatt ttgaggaatg
     1021 ccgtttatca ttattgaaag tcttctctga tcaaaacctc tgagtgtaag ataagaataa
     1081 cctactgatg atccggattc tgaatatgaa tttatacttg tatatccgtt tagcagcatc
     1141 ggaatatcct gcacgctgta cctcttttca atctctgctc tcttaatatt ttcaaatgta
     1201 atcggagtta atcttttgaa tccctttaaa gcatcgacat caatagtttc cgttttgtat
     1261 tcagttgaat ctttattatc cgatgtttct ttttctgtat tcacctgtgc aaaaataatt
     1321 tttgaaaaga ataaaactaa aatacacaga ataaaaaact gcatgtaaga ctttttcatt
     1381 ttacttttaa taaataaaaa aacgcttaaa tctttttgaa cccggtaaaa agatttaagc
     1441 gtttaaaaaa ttacatttcc ttcgccggca ttatccgtag caggttcgcg ggtttcatct
     1501 cagccttcag ttatcaaaag aaagcacccc gatatatatt attaaaaaga actttttgaa
     1561 attataaatt ctgattgttt atagaaagtc ttttatagag caaattgcac agcataaaat
     1621 atttaattct gattaatttt catgcttgtt tttaaatatt gaattcttaa cattaaaaaa
     1681 aattatataa ttattatttt ctttatataa aggatcagga ggagtaatat gcaaacgatc
     1741 aaaattataa tcatcgccat acttatttta gctgccgaaa attcattccc tcagacaggt
     1801 gttgaaacaa tatacagaga tacaattatc ggaatacgac aattcaattc ctttgaactg
     1861 ttaaatagtc cggataaaag aatttattta caggaagatg cagaatataa taataatttt
     1921 tttggcggga tatattatca gttggttaaa aattcaaatt ctttgataat tccttttaat
     1981 aatgcgctaa atccaagatt taccagtttc tgtaatttcc caaaaatata taatgtcccg
     2041 atagaatcct ttactgtttc taatcttagt gatcagatat taattttaaa ttcaagaata
     2101 agctgtgata caatttccaa tgacactacc ctgctctcta caaacagcgg catttctttc
     2161 agaagtattg atataaaaaa ctgtacagga tttggaatct ctaataatga ttcggttatt
     2221 tttacatgcg gtacttatga tctgatcaat tacgcttttc aaaaaagtac aaataaagga
     2281 cagacatgga taattacgga tacacttcag gatataaaga aactttctga cggtgggttt
     2341 ataaaatgca atcctttcga taaaaacata atatatattt cgggtaagaa taatttactg
     2401 ataagtacaa attcgggcag taattttatg aatacagcag cgccgtcatt tgataaaata
     2461 ttttttaata ataatgatca ttcattatac ggaataaatt ctgcatactt atttaaatcg
     2521 actgacaacg gattgagctg gcagataata tatgtttctc cgagtattga atttacagat
     2581 ctgcagattt ctgaagaaaa tccgaatata atttatgcgg ggacagataa tggaatattt
     2641 atttcaacaa atgccggtgt tgactggtct ctatataata atgcttttcc cggatcaaat
     2701 aaaatcttag ggctgtcaaa agatatcgga agtaattctc ctttatatgc ctgtacaagt
     2761 gacggtcttt ataaagttac gcatgcttat tatcagtcga ataattttat tacacatttt
     2821 aaaaccggga atttatttca gtactttgta aataatttac cgggaaaata ttattatact
     2881 gtaactgtag aaaaagatac tgtctttagt aacggtttaa aatattggaa attaataaca
     2941 aacaatacag cttcttcagc tacttatgaa tatatagatt ccatttcttt aaagtggctt
     3001 aaatatgaac cgtcatgtat taattcagat cattatggaa attctcttat cattgatttt
     3061 aatatggaca ctgggagtat ttttaaaata tgtaattgga ataatgtagt tacaagcaaa
     3121 acaatttcca ataacttttt aggcgatccg actttcttga gaaattccac ttcttaccct
     3181 tatccttttt cattcgatgt taatagagaa aaatatgtgg aaaaatttgg atacctggga
     3241 tcttcaggtt ttggttatag tgtaacttta accggagcgg taatagaagg aattacatac
     3301 ggagtcatta ttaatattga aaacaatcag tctacggaaa ttcccggaaa tttctcgctc
     3361 tctcaaaatt atcccaatcc attcaatcca aacacaatta taaaattcca ttgtcctaag
     3421 tcaggtcatg tttcattaaa aatatatgac gtacttggaa atgaagtcga agtacttgct
     3481 agtgaaaatg ttagcgccgg aagttattca gtagagtgga atgcggaaaa ttattcaagc
     3541 gggatttatt tttatagatt tactgcgggg agttttacgg atgtgaagaa gatgacttta
     3601 ctgaaataat ttcaaactac ttattttata tgtcttttca atggtctatt gttaaaatta
     3661 gtattcaatt ttatccgcca agatttaaga tttgtattct attcgtgccc ttcgtgccgc
     3721 tcactaaaac caacaacggc ggaaataatt gaaagcaccc cgatatttct taaaagaact
     3781 tttaaaaagt atagagaata aattattgaa attaagattc aatctcacca atgagaattt
     3841 atctccacaa tattaacttg tctgatcgtt acatcttttt acaaatatgt tattgctcag
     3901 taaatttact tcatttaaat ttgtataaac tattttacaa gtatgataac tagaaatatt
     3961 tatgcggctc tgtttatatt atttattctg aacatctctt cctgcagtga agaaaattct
     4021 ttatcggtca aaaatcaaat ttcatcagcc gacaaaatag agtttttata taaagacggt
     4081 tttgattcaa caggaaaaat gattgtaaaa acttcaaaca ttacaaatca gaaagatatc
     4141 attgagatta ttaatacagt atcagatgaa ccattcccgt atcagtattg catctccaca
     4201 ggctcaatga cattttacaa acaagattct gtattgatct cattagttta taatactaca
     4261 gatgattacc gacatattgc gtttaatcaa tataataaac tcacagccgt caaactttca
     4321 gaagaaaatg caagactact cgaattgtat tcggctaaat agaaataatt ttctttaaaa
     4381 attttttaaa tgaaaaaact ttttgtaatt tggatactga ttttatcagt ttcagcaaat
     4441 gctcagatca ctcctgagat ctattcatgg cagcaaaaca taaataatat aaccggttat
     4501 aatgggattc ccgctgatgt tcagcaggtg ttttattctg ataattattc ctatattaaa
     4561 tgtaccggca ttccatctta tactatcggt ccctgggcaa tgaatccgaa tattccgacc
     4621 aatcagaact ggacattcaa gataacccga tttcctgttc ctgacccaac tccttccact
     4681 gcaggaaatg gacagattgg tgttttaaaa aatggagtag tacttttcaa ttatggagat
     4741 gctatgtctt acaacaatct gaatatctgg cacagagttg ctcagtattt tgaagccgtt
     4801 agtttcgatt catcaggcgg acatcctgca cctggcggtg tttatcatta tcatattaat
     4861 atgaaatatc tctatactcc tgtgccttct gaacattcac cgatacttgg ttacatgttt
     4921 gacggctatc cgctgtatgg tccgtatgga ttttcagata caaacggaac cggaagtatc
     4981 agaagaatgg taagcggtta tgtaaaaaga aatatcacta cgagaactac acttcccgat
     5041 ggaactgtac ttcctcaaaa tcaatgggga ccaaatatca gcactcaata tcctctggga
     5101 tgttttaaag aagattatga attgaatacg aatggtgcag atctcgattc acataatgga
     5161 agattttgca ttacgcctga atatccaaat ggaatatatg cttattttat aactatcgat
     5221 tcacttggtg aacctgtata tccttacata attggtaata cattttatgg cgaagttgta
     5281 cccggaaatg ttcctcctgg cggtcatgta acgatcactg aacctgtgac acaatataca
     5341 ccacttagca tttccaataa taatgcttca aataataatg ctgaatcatt taatttgtat
     5401 cagaattttc caaatccatt caatcctgaa acaaatataa aatttgatat tctgaaaaca
     5461 ggatatacta ctctgaaaat ttatgataca aatggaaaag aaactgatat attgttgaat
     5521 gaaaacctta cacccggcag ttatatagta aaatttaatg cagcagataa tccaagcggt
     5581 atttattatt acaaactcag ctcaggaaaa tctgtatcag taaaaagaat gatgttgata
     5641 aagtaatttt tgaactttat tttcgtaaaa actttattga tccgttattt aattttttaa
     5701 attaaagaaa tgataatttc ctaaaactta attttaaaat cacaaacatg cctgaaaaaa
     5761 tctcaagaat tattttattt gtaaatgacg ttttaaaatg cacagaattt tacaaaaata
     5821 ttctggcatt aaaagtaata ggaattgcta acccagactg gactgagcta aaagccggca
     5881 actgttgtat tgcattacac aagaccgggc aaaaaggtaa aggggatttt cataataatg
     5941 tgaagatcgt attttataaa aaagatgtga aaaaagcaaa agagctgatt gaaaaaaatg
     6001 gaattaagat ggggaagata tttacttttg aaggaatgaa ctattgtgac ggaagtgatc
     6061 ctgagggaaa caaatttcaa ttttctgacc ggtgatattg atccgatata ttacttctta
     6121 gaaatctcaa ttcctttttc ccagatattt aatattttat tgatttccat tctaaacaga
     6181 ctttcattga tagtaaattt tatttttcct gtatctgtaa ttcgaccaat ataaatttta
     6241 tgctctttac caagaaaatt tgaaaactca ttaaaatcta tattctgatt tggcactagg
     6301 ttaaagatat ttgtaccttg ttcaatcttt tcaattctaa tcccatcaat tttattgatt
     6361 tcaactatta aattttctga caatgaaatg atttcctgcc atttagattc gatattcttt
     6421 tggaaatgaa gtgccatcgc agtattattc catgactgaa aaactgtacc gccatgtatt
     6481 tttatctgat gaggaatcct gtcaattact tctgaatccc cacatactac tgcaccgccg
     6541 gcagcattca gacatttata aagtgaaata taaattgtat caaaaaaaga tgaatattca
     6601 gaaataggga tattcgtata agcagatgct aaatgtatgc gtgctccgtc gagatgaagt
     6661 ttataaccat tttctctgca gaaatcagaa atttctttta ttgtttctat cggcacatac
     6721 tttccatctg cacgcctcac aggattttca attactacag tgcccgtacc gctgtcaaat
     6781 gtctctccgc tttttaaata atctaaagtt tcttttagat cgtccagttc aaaatatggt
     6841 ttaccttttc caataggtat taatcttttt ctatgaacac taaataatcc atctgcttca
     6901 tcacgaaaca tatgactgtt ttcaggaact atgacttttg tattttcacc attcagtaat
     6961 ttgacactca gatgatttgc catagtcccg gtcggaagat aaatacattt ttcctttccg
     7021 gttattacag caaactcttc ttccagtaat tttgtagatc cgccatttcc atataaatct
     7081 ctttcaattt tttcagacct gtttatttct gaaagtttct caatatactc ctcaggtgaa
     7141 aatccaatcc cgtctgtgat gaaatttata gatttaaatt tttgatccac attatttatt
     7201 tgaaggaatt ataaatttat tttcttttgg gaattttttt atcaaatctt ctccaacttc
     7261 aaaattagtc tgtagtaaac tatccggatt tgatctgagc cagtcactca gatctaatgc
     7321 cagataatct ccgtcattaa aacctaccaa aactattaac acttcgtcac ccgtattttt
     7381 tatataatgc cctgctccca tcggcacata accgatatca ccctcattaa agtcttctat
     7441 tactttattt ccctgtgcaa gaaagactcc catctgagct ttgccctgaa tataatactg
     7501 ccattcatca gcattcggat gccagtgcat ttcccgtaaa ccacccggct gaatttcaag
     7561 tactgaacct gccattgttt tgctgatcgg aaattctttt tgagttacag tcttttgtgt
     7621 tcctcctccc ggaactcttc tcggctcctg agccagcaaa gggtaacggt gtttagttgt
     7681 cagctcaggg tcacttactg atgctgaagt aaaagattga tcatcaggaa ctttcccttt
     7741 tgcaaaatac acctcacgtt taggcagcat tcctacttca tcaagcgtga tccctaaatt
     7801 ctgagcaact atttcaggtg gagtggaagc aataaaatct gtaatactga atgtgtgatc
     7861 ttcagaaaaa tctccattat caaaaattaa aatgaagtga cattcttcat cacttattcc
     7921 ctgtatggaa tgtccatatc ctcttggaaa ataccataca tctcccggat caaatgtatc
     7981 tataaaagat gtaccgtccg gatgtaatat agttgtccga cagctgcctt tgattacata
     8041 accccattcg gcagcgttag catgccagtg aagttccctc ataccgcctg attcaagtct
     8101 catggaaact cctgcaattc ctacggaagc aggaaaatca acaactgaag cacctctggt
     8161 tattcccccc ggtaatattt ttggttcctt tttctcaagt tcatacttaa aggagatcat
     8221 aatatttaat ttttgttttt taaaagtttt tgtaataatt aaaaaataaa tcttctgtat
     8281 aaaagatatt tttctctgtt tgtaacttgt aaaacgtaac tcgtaactcg taattcttaa
     8341 ttcctaattt ttaattctta attcttaatt caatctttgt gtttcatcgc tttcatcact
     8401 tcaaagatat gcaaaacaaa tggaaacaat gcatccatac tttctttagc accctttgta
     8461 gaacccggaa gtgcaagcac caatgtatct ccgatcaatc ctgagacact tcttgaaagc
     8521 atgctgtatg gagttctttg ctgaccataa cttctcattg cttcttcaat acccggaatt
     8581 tttctatcca gcaatggctc gatcgcttca ggcgtaatat ctctcggtga taatcctgta
     8641 ccgccggtaa atattatcat gttaactttt tcatctgaaa gttttaatac tttacatttg
     8701 atatctgccg cttcatcggg tatgattgta tattcgaata tatttatttg taatgattca
     8761 agtttactga ttatgacttt acctgcacgg tcttcctttt tttctgatga gatagaatct
     8821 gaacaaacaa ttacagccgc attcaaagct tttctaaatt cttcaggaaa atctgtctta
     8881 ccgcctttct tctcaattaa ttttatacta gatatctcaa tatttttatc aatcggcttc
     8941 agcatatcgt aagctgtaag tgcagcaata gaaactccat gcattgcttc tacttccaca
     9001 ccggttttat aaatagtttt tacttccatc tctattttga tctcgagtcc ttctatactg
     9061 aaatttacag ctgcaaactc aatcggcaat ggatgacaat caggaattac atcactcgtt
     9121 ttttttactg caagcaatgc agccgcctta ctcatttcaa acacatcacc ttttgcaaca
     9181 agtttgttat tgatagcatc tattgttgat tgactgctta cctttactga cgcctgagct
     9241 tttgcatatc gaagtgtatt aatcttgccg gttatatcca ccattgtttt atatcttaaa
     9301 aaaattttct aaatgcattc tctataattg tagctgcagg ttttagactg catacaaata
     9361 aagtttaaat tgtaaatatt aaatatcaat attatttgtt caattaaata taaatttcta
     9421 tataaataat attaacttat gctttttgca aatataaata ttttatgaat tcactttcca
     9481 tgaatattca tcacctgcaa atatttcctt tccccataca ggtaattttg ttttgataag
     9541 ttctaccatt tctctgcatg cttcagttgc atcttttctg tgaactgaag aaacgaatac
     9601 gaacatatta atttcacctg caaaaacttt cccgagactg tgatatacat gcatacagat
     9661 cagatcatat tttgcaaaaa taatttcacg gatattaaaa tattcctctt ctgccatttc
     9721 attgtaagcc gaaaattcta ttgcctgtac tatcctgctg tcaatttcat cactccttat
     9781 ctgtccgaga aatatctgat gtgcacctat atttttcttt gtactgtgtt ttgaaattga
     9841 atcagctata aactctggtc tgatcgcacc ctctaaaaat aattttttcg atttcttttt
     9901 atccatattc atattttctg atgctttgat aactcgattg gcgtattaat atttgtaaaa
     9961 aaatttttat tgttaaaatc atattcatca aatttatata atactgtttt caaactattc
    10021 aaagcatttt tcattttcaa atcattgttt tttataagtt cttttatttg ttccagacaa
    10081 tttttatcat agaccgcaaa taaaggttct gcttctccat tattttcaaa gacaagtaca
    10141 tcttctccat ttgctttatc aattaatatt gacaaaacat cagaagttat aaatggagaa
    10201 tcacagctca gtatcaatgt ttttctcgtc tgagaaaaag taagtgaagt aagaattccc
    10261 ccaagtggtc cggcattctt gatcagatca ctttggcagg aatacccgaa ctgttcatat
    10321 tcaggattat tagtaatgat aataatttta tccgaaattt tttttacctc atcaataaca
    10381 tattcaataa gcattttatc attaagatac ataagacctt tatcctctcc catccttgaa
    10441 cttttccctc cggcaagtat tgcaactgtg acattatttt ttatcataaa cttttaggta
    10501 atatatgtgt ctctacataa cttccttttt ttaaaagttt attacctgaa tttaaaaata
    10561 ctatcgcatt tgattcagta aatgatctca atatatttga cccctgacct tccagtatcg
    10621 taatcatatc attttcaatc tttgctttca agaataaatc cttttctcct ttcaattcat
    10681 aaggtttaga taatggaaga aattttttgt tcaattcaaa ttcagaatgt ccgctcattt
    10741 tattaagaca tggaagtacg tattcataaa agcatgtaag tgaagcagca ggattaccgg
    10801 gcaatgcaaa tactaattta tctttgtact tcccagcata gagcggcttt cccggctttt
    10861 gtaaaacttt ataaaaaatt tctctgactc caagattatg aaggatttct tttacaagat
    10921 catattttcc tactgatatt cctcctgata ttagaatcac atctgaagtc ttaatcatat
    10981 tgtttataca gaaagatatt ttatctttta catctttgac tgaatttatt tttgaaattt
    11041 catatccgca ttctttaata acagattcca gcatcaaaga attggattca tatattttac
    11101 ccggtgataa ttttttaccc ggtctctgaa gttcgttgcc ggttgacaaa actgaaagta
    11161 tcggtttatc tattactttg attttgtcaa ttcccattga gcataaaaac ccgattgatg
    11221 caggatttat ctgatgacct tttgttaatg cgacttgacc ttttttgatc tcagatcctt
    11281 tgctccggat attactaacg gttttcagat cagataaaga aatagtgaca ctgccttttt
    11341 ttactacagc tttttcctgc attaccacac acgtagaatt tataggaact gctgctccgg
    11401 tgtaaatata tacagccgta ttttttttta gaaattttat tggtttgtcc ccggctttta
    11461 tttctccaat aatcaaaaat gaaaattcat tattcatatt tccgactgta tcatcaaagt
    11521 taactgcaaa gccatccatt gcagactgat caaaaggagg cagatcgacc ggtgacttaa
    11581 ttgattctga taaaaaataa cctgtaatat ctgaaatatt ttttattgtt gcaggcattt
    11641 ttttcacaga cttatataat ttattctttg catctttgac actgatcatt tttctaaaga
    11701 aataatttag tatgtgtaaa tttatatcta gtaaagtttt tatatgatta caaaaataac
    11761 gagatagctt ctaatatcca ctgataaaat ataaattatt cctgtacttt tataacttat
    11821 cggtaaaatc aattttagat catgttgcat tattgtatag aaaactttaa ttttcactgt
    11881 caaaatttta caaagaaact gtaaatagat ttttagtata tttgtaaaag tttttaataa
    11941 tatttattta gtatggatag aataaattta gaaaaagata tccgtgtatt ttatgttcag
    12001 gctgaatcct ttcccgatgg aattcaaacc gcatttaaaa aactgcaaat cctgatcgac
    12061 acgggtaaag acagaaggtg ctttggaata tcccgacctg aaaataacga aataatatat
    12121 cgggcggcgg cggaagaatt atttgaaggt gaagctgaaa aattaaactg tagaactctt
    12181 gttattaaaa aaggtaatta taattttatt actgtcaaag attttatgaa taatattcaa
    12241 gagataggta aaagctttga aggtctttgt tctctatcaa atacagatcc cgatggttac
    12301 tgtgtagaat ggtactttaa tgataccgat gtaatgtgta tggtaagatc aactgattaa
    12361 ctttcgggat aatttaaatt taattttcat gaaaataaaa attgcacttc ttttttttac
    12421 tgcagcttta attttaattt cagatgtatt ttctcaacag gactcttcag attttaaatt
    12481 cattactcat aaaaataaaa aagacagcac tcttttaccg ttttatactg aacaaggaga
    12541 ctggttccga aatcccgaga taccaagttt tgtgttccag ggacctgata aaaaattttc
    12601 acttggtatc ggcggctttt ttaactatac agcttcctat gattttgacg gcatttcaga
    12661 taatctcgat tttgttactt atgatataat tgtcccaaat gatacaattc aaaaatcaca
    12721 gtttcagatg tatgcaaata cttcactttt atatttcaaa gtaattacca aaacttccca
    12781 tggaaattta gtaggttatg tcagcgcgaa tttccggggt gatggtaata attttaattt
    12841 atatcagatg tatatcaggt atatcggatt tgatcttggt gtagactgga gtactttttc
    12901 agatgctgct tcatggcctg taacaataaa ttatgtcgga ttgaattcaa tgtctgatgc
    12961 attaagtaca cttattcgat actcatacaa atttgcaaat ggttggaaat ttactcttgg
    13021 ggtggagcaa ccggaattta atactgcttt cacaaatacc ggcagtttaa aacagacaat
    13081 tcccgacatt cccgcatatc tgcaatacag ttttggaaac agtcatgtca gactttcggg
    13141 actgtacaga aatttatttt acagagataa tatcagtaat gcagacagaa ccgttacagg
    13201 cactggaatt tctatgacgg gaaatgttga gatcacaaaa aaagctcaga tattttttca
    13261 gggactttat ggaaaaggga tcggcaagta tgttcaggat ttaagtatag atgatctaag
    13321 tgtcactcca gtttctgcac atgcaggaga actgacagca cttccggtat atgcatatta
    13381 tggagcactg cagtataatt ttaattcaaa gttttttacg actataatgt acagtcagct
    13441 taaagttcag ccggataatt tttactctcc ttcatcttac agctttgcac gacagttcac
    13501 aggtaatttt ttctggcagt ttttaccagc ggctaatgtt ggactggaat ataattttgg
    13561 agacaggacc aatcaaaatg gacagaattc aattgcaaac agagttgagt ttatgatgca
    13621 gtataatttt taacaatctg atattaccta atttatagat ccatttttaa ttaatacaat
    13681 tgtatataaa aattatttat atagtaaatc tttatgtcaa tatttatttt agtccattac
    13741 caatttttca aaagtcatac ttcctaaatg catgagaaaa atcacaaaca cgacatagct
    13801 ccatggagac acaaacttca cgaagttata tttgaagcag acactcctac cggtaagtgg
    13861 tttgatatta ttctgatcat aagtataata ctgagtgttg tcgttgtgat gcttgatagt
    13921 attcaggttg tgaagattga aaacgctgag ctgctgtttg cagcagaatg gtttttcact
    13981 attctgttta caattgaata tattctgaga ttattgtctg tcagcagacc acttcattat
    14041 gctaaaagtt tttttggaat tattgatctt cttgctattc ttcctacata tataagtctt
    14101 ttttatccgg gcggtcagta tttagtagta atcagacttt taagaatact caggattttc
    14161 agagtattaa agctcgttca gtatgtgggt gaaagtaaaa ttcttattca ggcactaaaa
    14221 ggcagcagac ggaaaattac aatttttatt tttacggtac ttacaatagt cacaattctt
    14281 ggatcgctaa tgtatctgat agaaggtgaa gaaaacggat ttaccagcat tccccaaagt
    14341 atttactggg ctattgtaac tcttactaca gttggttacg gtgacattac tccgcagact
    14401 gttcccggac aatttcttgc tgtaattatt atgatacttg gctatggaat aatcgctgta
    14461 ccgacgggaa ttatgacagt tgaaatgaac aaggcgttca aacaaaaagg cggaattcgt
    14521 gcatgtataa actgcggagc agataatcat gatgaagatg ctgtttattg taaatactgc
    14581 ggaaaaagtc tttaaaaaaa cgttctcata aagatctatt tagttgttat ttaaaatgaa
    14641 tccatcaata ctgttggata atatatggag taaaaaaaca gattttaaaa atatatttta
    14701 tacgataata aagaaaaaga aattgtgaat taataaaatt tcaa
//
LOCUS       DAORVQ010000076        14712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1830_length_14712_cov_5.662414, whole genome shotgun sequence.
ACCESSION   DAORVQ010000076 DAORVQ010000000
VERSION     DAORVQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14712
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1830_length_14712_cov_5.662414"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLX80_07740"
     CDS             <1..663
                     /locus_tag="PLX80_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20714.1"
                     /translation="KFSLAGGPTIGWQWQNVDDLNAQMTAIGVPGFPKDGFLTLGGGG
                     FVELPIKGLDWLRLGGYGTGFTYERSYTDQVDSLSKTVYYSFGAGGITLDYVLPVTKA
                     FDMTFGAFLSTGNLTIQVYQNTLNYGNWNVIFGEIAAGAPSENISRKLTSRFYSVQPQ
                     IGFGLFISKLIYAKLNAAYLFSAQNQWKADNDTPVTNVPEGIKPNGFNINFGLNFGLF
                     TK"
     gene            668..1693
                     /gene="rfbB"
                     /locus_tag="PLX80_07745"
     CDS             668..1693
                     /gene="rfbB"
                     /locus_tag="PLX80_07745"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQY20715.1"
                     /translation="MKNILVTGGAGFIGSNFIKYALLNTDFNLVNFDKLTYAGNLENL
                     TDVEFNDRYKFVKGDICEKTEVKKAVEENEIDTIINFAAESHVDRSILGAKEFINTNV
                     MGTHVLLEVLKDNKLEKYLQVSTDEVYGSLPEDKKEILFTESTPITTNSPYSASKASA
                     DLLCNSFHHTFGLPILITRCSNNYGPYQFPEKLIPLMIVKTLNGEKLPVYGDGKNVRD
                     WLYVEDHCSAIIKVLTDGKIGEVYNIGGNNEWYNIDIVKLILNRLGKTDDMIQYVKDR
                     PGHDRRYAIDSTKIMTELGWEPAYDFEKGIALTIDWYIKNEDWWEKVMSGEYQKYYTE
                     NYGNKVS"
     gene            1806..2747
                     /locus_tag="PLX80_07750"
     CDS             1806..2747
                     /locus_tag="PLX80_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY20716.1"
                     /translation="MSIKVENLSKYYGQQAAVNNISFEINTGEIVGFLGPNGAGKSTT
                     MKMITTYLTPNNGKIWVNDLDTEEDSMGVRRKIGYLPEQNPLYLDMNVMDYLEYAAEL
                     ESVPKSDIPKAVKKMVEVCGLGDVQHKDIGELSKGFRQRVGLAQAMIHDPEVLILDEP
                     TSGLDPNQIIEIRNLIKKLGKEKTLLLSTHIMQEVEATCDRVLIINKGEIVADGSPDT
                     LQVKFKGQLEITLILKKDSTNKDLILKAVSSIKNIEKAILLKEEEDALHFSLTGLKGS
                     DVREEIFKKIVSMNMVMLGLHQEETSLEDIFRQLTSN"
     gene            2782..3492
                     /locus_tag="PLX80_07755"
     CDS             2782..3492
                     /locus_tag="PLX80_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY20717.1"
                     /translation="MNNILTIFKKELRSYFNSPVAYIVLVVFLIITGWFFTSNLFLSG
                     VVSMRNVFDIIPFIFLFFIPAISMRTFSEEKKSGTIELLLTKPITDMDIVLGKFLATV
                     ALTTVALAITLIYVFSLTFLGPIDTGAIIGAYIGLLFMSGIYVSIGIFASSLTENQVV
                     AFIISFLIVFGLFLLNKVLIFLPPELSSILEYISVDYHFSNIARGVIDSRDIIYYLSG
                     ITIFLLFTRTSLESRRWS"
     gene            3529..5139
                     /locus_tag="PLX80_07760"
     CDS             3529..5139
                     /locus_tag="PLX80_07760"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HQY20718.1"
                     /translation="MNKKDILIKLAIVIGIIIVINVISKRVFTRVDITKNNSYTLSPI
                     SKEVVSTLDDNLLIKAYFSDNLPAPYNNVRRQVQDILDDYRSYSKGNMNYEFLNPTSS
                     GEGEGNELDQEAQKFGIQPVQIQAMDNDKLEVKRAYLGLVLLYEGKQEVIPVVQNADN
                     LEYELTSLIKKMISTEKKKVGFLTGHGETELSKLAQINAALSAQYEVTSVNLNESVSL
                     DPEMKVLIVMAPSAPFSESDKYKLDQFVMNGGNVAFLMNRIVPNFQQQQMVIGNEVTN
                     NIEDLLLNYGIRINTDLVRDLQCSPVQVQSAIGFPISVNYPFFPVITDIARDNPAFKN
                     IKSVVLTYSSTIDTGIAAGKNLKINPLLVTSDKSGLVVDFFFLNLEQFQNMTKQSADT
                     MFDKRGFLVGATYEGQYKSFYEGKEMPSDTLSGWVPLSGERLNQSRSNSKIIAIGDGD
                     FANEENKPPRENIVFFINMVDYLMDDVGLAEIRTKMSSESPIEETSEETKKFLKYFNL
                     IFPPALILLIGLYKWNQRKQKKKNLQKS"
     gene            5164..6069
                     /locus_tag="PLX80_07765"
     CDS             5164..6069
                     /locus_tag="PLX80_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HQY20719.1"
                     /translation="MKSSKTYLYLGIFAALLVAAYFITTDRGEKTSSYKLTEQKLFEL
                     DSVKADRIEILNNGNKVVLSKATGEWRLLEPKDYRVVSANVENAVSNLSSMKLESIVS
                     KNPEKKVAYGFDEPNVTTITVFESGNKISEFLVGNAASTTASFVKKTDSDVIYLAESF
                     LRDNFVKPNNNDWLDKSIISIPKPAINSVEIIMPGESFTVARDTSGNYMIGNEPVGKS
                     FDGYLNLFERLQTTDFSDTTLTEESNFTNKIIIDAGNKTELNLLKLDTQPVKYLLMIP
                     GDKQIYELEEGYANNLLKTKKDLLQ"
     gene            6130..6822
                     /gene="plsY"
                     /locus_tag="PLX80_07770"
     CDS             6130..6822
                     /gene="plsY"
                     /locus_tag="PLX80_07770"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQY20720.1"
                     /translation="MLNLVGIIVTSYLVGSIPFALIVGKLFKKIDIRNYGSGNLGSTN
                     AFRVLGVPLGIIVQLLDISKGLFAVLVVTRLFYPDLPFENYTPFEDITVLKIISGISA
                     VLGHTFSVFVKFKGGKGINTALGMLISLAPVEVGVSVGFFIVTLLFSGYVSLGSIVAS
                     SVFPVTMFVRENFFKVDIYGYNTLIFFSIGISLLLIYNHRENIKRLLYGNENRFDKLW
                     LIRIFKIKAPFS"
     gene            6833..7831
                     /locus_tag="PLX80_07775"
     CDS             6833..7831
                     /locus_tag="PLX80_07775"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQY20721.1"
                     /translation="MKIAVLGAGGWGSALAILLNQNGNKVTLWEFNPKYAETLSLHRE
                     NFYYLPGIKIPKTIDITNDLEYAVKDKSLVVISTPTQFIRNSLSRLKNFDFKDSLLVS
                     VSKGIEISSNFLVSDILTDIFGGIKRENILCLSGPSHAEEVSRKLPTAVVCSSSDNNK
                     SRIVQKVFSNSYFRVYSNDDIIGTEVCGALKNVIAIAAGISDGTGFGDNTKAAIMTRG
                     IHEIMKLGLKMGAKKETFFGLAGIGDLIVTCGSDHSRNRRVGIQLGEGKKLKDILSES
                     KMVAEGVATTKAAYDLSKKLNIELPITHQVYDILYKNKKPIKATELLMTRNLKEEF"
     gene            complement(7959..8840)
                     /locus_tag="PLX80_07780"
     CDS             complement(7959..8840)
                     /locus_tag="PLX80_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQY20722.1"
                     /translation="MLKYKADLKTLLFLSLTTAMLFFQWNFGFHIITYIISLYLAVTI
                     AVIAHNHNHVPMWKSKLLNHITDYWITLFYGFPIFAWTPTHNKNHHKFNNRDGDFTIT
                     YRVSEKNNIFTLLSYPTISSYFQQNPIVVYLKFLWNNKRKKFFLSIFQYVALGLFIIV
                     ALLIDWQKALLYVIIPQQFALFSVLIFNYIQHVHADEESDYNHSRNFVSPTTNFMLFN
                     NGFHTIHHFRASIHWSETPEAHKSIDHLIAPHLKERGFWPYIIKAYIIAPFNKTFRTK
                     SMRLERILKEEKKSAHV"
     gene            complement(9089..10519)
                     /locus_tag="PLX80_07785"
     CDS             complement(9089..10519)
                     /locus_tag="PLX80_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20723.1"
                     /translation="MKKITFLIMLLVLSIASDSYSQYGNSRAIVNTTFNAQFVGRNVT
                     FAHCNEGVAAGYAGVIPATLDGVVNTPFYCLDVCTPIALGDTIKDSASSIPQAIYITN
                     TYYPSRTTYPGKLANNNDEGSAVQLAIWHFRNNVDPDSITAITGGTVTVADFILRVKE
                     IIADVNLNGGSTIQVSTLEIKPGSDPDDFYIETLDTAGNPIAVASITLAINGTGTLSQ
                     YTVSTNAFGVSDTVTVTGASSGDVISASATLEVPGGTTYSSAEVLNPVLQLLVLGRTT
                     TAIRSTETTWGALPVELSSFTAGISGNDIMLNWSTSSEINNSGFEIERYKTGTNAWDK
                     VGYVTGNGNSSVSQNYSFNDKNLTSGIYTYRLKQIDYNGNFEYHNLNGEVVIGVPNNF
                     DLSQNYPNPFNPSTKIKYQIPSDAFVTLKVFDNSGKEVATLVNGSMSAGFHSINFQGS
                     NLSSGVYFYKLETNGFTKVMKMALVK"
     gene            complement(11028..12428)
                     /locus_tag="PLX80_07790"
     CDS             complement(11028..12428)
                     /locus_tag="PLX80_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20724.1"
                     /translation="MKKILLLVALLISISNGISIAQLTPASVEGISPKRNITFIDPIT
                     NQSETRTVGLFYGKIYNPNSGPDVCFYSIDIIKAPSSCLPNFDYVDDSTGILPSRVCY
                     ILYNYYPAATGPGQLSNLANESGAIQAVIWHYMNGLDLNSITSSAVRNRANAIKANVD
                     VNGNNSVVPEVIKIVPDFDPDFFFIETKNDAGSGVAVNGIQLTITEGSLSTYTVNTDA
                     SGISDPIQVIGTGVGTITATADNIILPKGRIFRHINNDCPKVVLACPGPGELRVYSDW
                     GALPVELTSFSSSVNNNNVSLNWITASELNNSGFDIERMSVTTGEWKVAGNVLGNGTT
                     LNSSEYTYTDKNVTSGRYSYRLKQIDFNGNFEYFNLTNEVEIGTPSKFELSQNYPNPF
                     NPETKIKYELPNDGFVSIRVFDNSGKEVAVLVNNNVSAGYHTVNFKASSLSSGIYFYR
                     LETPGFVKVMKMALVK"
     gene            complement(12785..14167)
                     /locus_tag="PLX80_07795"
     CDS             complement(12785..14167)
                     /locus_tag="PLX80_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20725.1"
                     /translation="MKKVIFSLVILVVLVFTSISYSQYGLSDAIITGTGAGKSVTFID
                     PYTNASRQVFAGQMNGSVDNNATKFYCIDLRRTLSFPDECHKDSAVSSSKIVYVLNNY
                     LPYNPDPVGELPNLNDEVASTQCAIWHFSDGMDANTITDNTIKVRTLEIIADADANGV
                     ITNIVTTFSIEPGIGIDEIYIKTLDQNGDPIAINNIQLSISEGSLSTNNVNTTLPNGE
                     SPAITVLGTGTGIITATAMCMIPQGVTYACPNKQRLVIAAPAIGEKEATFDWGALPVE
                     LSSFTSVINSRNVTLNWSTSSEINNSGFDIERKVSTNETWSKVGNVTGNGTTNETRSY
                     SFTDKDLSAGQYSYRLKQVDFNGNFEYFNLSNEVVIAAPDEYSLNQNYPNPFNPSTKI
                     SFQIPVDGAVSLKIFDISGKEVLELVNGNLNAGFHTINFNATGLTSGVYFYKLSAGNF
                     TKVMKMSLIK"
BASE COUNT         5028 a         2308 c         2602 g         4774 t
ORIGIN      
        1 aaattttcac ttgcaggcgg tccaacgatc ggctggcagt ggcaaaatgt agatgatctg
       61 aatgctcaga tgacagctat cggcgtaccc ggatttccga aagatggatt tctcactctt
      121 ggaggcggtg gatttgttga acttccaata aaaggactgg actggctgcg tttaggcggt
      181 tatggtacag ggtttactta tgaaagaagt tatactgatc aggtagatag tctgagcaag
      241 acagtttatt attcatttgg agcaggagga attactttag attatgtact gcctgtaacc
      301 aaagcttttg atatgacatt tggtgctttc ctgtcaaccg gaaatctaac aattcaggta
      361 tatcaaaata cactaaatta cggaaactgg aatgtgatat ttggtgaaat tgcagccgga
      421 gctccttcag aaaatatatc aagaaaatta acttccagat tttattcagt acagcctcag
      481 atcggatttg gattatttat ttcaaagctg atatatgcaa aactcaatgc agcgtatctt
      541 ttcagtgcac agaatcaatg gaaagctgac aatgacactc ctgttactaa tgtacctgaa
      601 ggtattaaac ctaacgggtt caacataaac tttggactta acttcggatt attcacaaaa
      661 taaataaatg aaaaatattt tagtcaccgg cggtgccgga tttattggaa gcaattttat
      721 aaaatatgca ctccttaata cagattttaa tcttgtaaat tttgacaagc ttacttatgc
      781 aggaaatctt gaaaacctta cggacgttga attcaacgat agatacaaat ttgtaaaagg
      841 tgatatctgc gaaaaaacgg aagtcaaaaa agcagttgaa gaaaatgaaa ttgacactat
      901 cataaacttt gcagcagagt cacatgtgga cagaagcatt ctcggagcaa aagaatttat
      961 aaatacaaac gtaatgggaa cgcatgtttt gcttgaagtt ctcaaagata ataaacttga
     1021 gaaatatctt caggtatcga ctgatgaagt ttacggttca ctacccgaag ataaaaaaga
     1081 aattcttttt actgaatcta ctccaattac aaccaacagt ccttattcag caagtaaagc
     1141 atctgcagat ctgctgtgta actcatttca tcatacattc ggactgccga ttctgattac
     1201 aagatgttcg aataattacg gtccgtatca gtttcctgaa aaacttattc ctctaatgat
     1261 tgtgaagact ttgaacggcg aaaaacttcc tgtctatggt gacggaaaaa atgtacgaga
     1321 ctggctttat gtggaagacc attgttcagc aattataaaa gttctaactg atggaaaaat
     1381 cggtgaagta tataatatcg gcgggaataa tgaatggtat aatattgata ttgtaaaact
     1441 gatattgaac aggcttggca aaacagatga tatgatacag tatgttaagg acagacccgg
     1501 acatgacaga cgatatgcca ttgactctac aaagatcatg acggagctcg gctgggagcc
     1561 tgcttatgat tttgaaaaag gaatagcact tacgatcgac tggtatatta aaaatgaaga
     1621 ctggtgggaa aaagtaatga gcggcgaata tcaaaagtat tacacagaaa attacggaaa
     1681 caaagtttcc tgaataaatc cctttcataa caagtatata atgtaattaa acctttgcgg
     1741 gaattcaaaa ttggtttgaa ttttttttta taatgagtta tttttaacga tattttaaaa
     1801 attttatgtc aataaaagta gaaaatctat cgaaatatta tggacagcag gctgcagtaa
     1861 ataatatttc atttgaaata aacacaggcg aaattgttgg tttcctcggt ccaaacggag
     1921 caggaaaatc cacaacaatg aaaatgatca caacatatct tactccaaat aatggtaaga
     1981 tctgggtcaa tgatttagat acagaggaag attcaatggg agtcagaagg aagatcggtt
     2041 atctccctga gcaaaacccg ctttatctcg atatgaacgt aatggactat cttgaatatg
     2101 cggctgagct ggaatccgtg ccaaagtctg acattccaaa agctgtcaaa aaaatggttg
     2161 aggtctgcgg attaggcgat gtgcagcata aagacattgg cgaactttca aaaggattca
     2221 ggcagagagt tggtctggca caggcgatga ttcatgatcc ggaagttctg attcttgatg
     2281 aacctacatc aggacttgat ccgaatcaga taattgagat cagaaatcta attaaaaaac
     2341 ttggcaaaga gaaaactctc ttactttcta ctcacattat gcaggaagta gaagctacat
     2401 gcgacagagt attgatcatt aataaaggag agatcgttgc tgacggttcg cctgatacgc
     2461 ttcaggttaa attcaaagga cagcttgaga tcacacttat acttaaaaaa gattcaacaa
     2521 ataaagatct tatacttaaa gctgtatctt caattaagaa tattgaaaaa gcaattttac
     2581 ttaaggaaga agaggacgct ttgcatttta gtcttacggg gttaaaggga tcggacgttc
     2641 gtgaagagat atttaaaaaa atcgtttcaa tgaatatggt gatgcttggt cttcatcagg
     2701 aagaaacttc acttgaagat atattcagac aattaacatc taattaaact ttttgaatta
     2761 agaattaaga aattattata aatgaataat atattaacaa tatttaaaaa agaattaagg
     2821 tcatatttca attcaccggt agcttatatt gtactggttg tatttttgat aataacaggc
     2881 tggttcttca caagtaatct gtttctcagc ggtgtagttt caatgagaaa tgtttttgat
     2941 ataattcctt tcatattcct tttctttatc cctgcaatat ctatgaggac attttcggag
     3001 gaaaaaaaat caggtacaat cgaactgctt ctgaccaaac cgatcactga tatggatatc
     3061 gtacttggaa aatttttagc gacagttgca ttaactactg ttgctttggc aatcacactt
     3121 atctatgttt tcagtctgac atttctcgga cctatagata ctggtgctat cattggagct
     3181 tatattggat tactttttat gagcggaatt tatgttagca taggaatctt tgcatcatcg
     3241 cttactgaaa atcaggtagt tgcattcata ataagttttc tgattgtatt cggattattc
     3301 cttttgaata aagtgctgat atttttaccg cctgagctct cgtctatact tgagtacatc
     3361 agcgtggatt atcatttcag caatatcgca aggggagtca ttgattcaag agacatcatt
     3421 tattatctga gtggaatcac gatattcctc ttgttcacac ggacatctct cgagagcaga
     3481 agatggtctt aaatatttta agtttaaatt taattttggt ttttcataat gaataaaaaa
     3541 gacattttaa taaaactcgc aattgtaatt ggaataataa ttgtaattaa tgttatttcc
     3601 aaaagggttt tcacaagagt agatataaca aaaaataaca gttacacttt atcgccgatc
     3661 agtaaagaag tagtatcgac attagacgat aatcttctga taaaagcata cttcagtgat
     3721 aatcttcccg ctccatataa caatgtcaga agacaggttc aggatatttt ggatgattac
     3781 agatcttatt caaaaggaaa catgaattat gaattcctaa atccaacatc aagcggtgag
     3841 ggtgaaggaa atgaactcga tcaggaagct cagaaatttg gtattcagcc ggttcagatc
     3901 caggcaatgg ataatgacaa gctcgaagta aaaagagctt atcttggatt ggtattgctt
     3961 tatgaaggaa aacaggaagt tatccctgtt gtccagaatg ctgataacct cgagtatgaa
     4021 ttaacaagtc ttattaaaaa aatgatttcg accgagaaga aaaaagttgg atttcttaca
     4081 ggtcacggag agacagagct tagtaaactt gcacagatca atgctgctct aagtgctcag
     4141 tatgaagtca catctgtaaa tctgaatgaa tctgtatctc ttgatcctga aatgaaagta
     4201 cttatagtta tggcaccaag tgctccgttt tcagaaagtg ataagtataa gttagaccag
     4261 tttgttatga acggagggaa tgttgcattt cttatgaaca ggatcgtacc gaattttcag
     4321 cagcagcaga tggtgatcgg aaatgaagtt acaaataata tagaagatct tttattaaat
     4381 tatggtatcc ggatcaatac agatctggta agggatctgc agtgctcacc tgtacaggtt
     4441 cagtctgcaa ttggtttccc aatctcagtc aattatccgt tcttccctgt gatcactgac
     4501 attgcccgcg ataatccggc atttaaaaat attaaaagtg tcgtactcac atattccagt
     4561 actatcgata caggaatagc agcaggaaaa aatcttaaga taaatccttt acttgtaaca
     4621 tcagataaat caggtctcgt agtggatttc ttttttctga atcttgaaca gtttcagaat
     4681 atgaccaaac agtctgctga tactatgttc gataagagag gtttccttgt tggtgccaca
     4741 tatgaaggtc agtataaaag tttttatgaa ggaaaggaaa tgccgtctga cacattatcg
     4801 ggatgggtac cactttcagg ggaacgtctg aatcaaagtc gaagcaacag taagatcata
     4861 gctatcggtg acggtgattt tgcaaatgag gaaaacaaac caccaagaga aaacattgtc
     4921 ttttttatta atatggtaga ttatcttatg gatgatgttg gtttagctga gatcagaact
     4981 aaaatgtctt cagaatctcc gatcgaagaa acttctgaag aaacgaaaaa gtttttgaaa
     5041 tattttaatc tgatatttcc gcctgctcta atacttttaa taggtttata taaatggaat
     5101 cagagaaagc aaaagaaaaa gaatttacaa aaatcttaat catatttaac acatatattt
     5161 taaatgaaaa gtagtaaaac atatttatat ctcggaatat ttgctgcgtt actagtagca
     5221 gcttatttta taactacaga caggggagaa aaaacatcca gttataaact gacagaacaa
     5281 aaattatttg agctggattc tgtgaaagct gacaggatag agatcttgaa taatggaaat
     5341 aaagtcgtgc tttctaaagc taccggtgaa tggaggttat tagaacctaa agactacaga
     5401 gtagtttcag cgaatgtaga aaatgcagtc tcaaatcttt caagtatgaa attagaaagt
     5461 atagtttcta aaaatcctga gaaaaaagta gcgtatggtt ttgatgagcc aaatgttaca
     5521 acgatcacag tatttgaatc gggaaacaaa atatcagaat ttcttgtagg taatgcagct
     5581 tccacaaccg catcattcgt aaagaaaaca gactcggatg ttatatatct tgcagagagt
     5641 tttttaagag ataattttgt aaagcctaat aataatgatt ggcttgataa gagtattatc
     5701 tccattccta aacctgcgat caattctgta gagatcataa tgcccggtga aagttttacc
     5761 gttgcaaggg atacttcagg aaattacatg atcggcaatg aaccggtggg aaagagtttt
     5821 gacggttatc ttaatctgtt cgaaagactt cagactacag atttcagtga tacaacatta
     5881 accgaggaat caaattttac caataaaata atcatcgatg ccggaaataa aaccgaattg
     5941 aatctgctga aattagatac tcagccggta aaatatttgt tgatgatacc gggtgataag
     6001 caaatttatg agttagaaga aggatatgca aataatcttt tgaaaacaaa aaaagatctg
     6061 ttacagtaac ttttttttct ttgtaaaaac ttgaatatct ttttaataat tttactgcta
     6121 atttaacgta tgctgaattt agtcggaata atagttacga gttaccttgt cggttcaatt
     6181 ccgtttgcat taatagtagg aaaacttttc aaaaagatcg acataagaaa ttacggaagc
     6241 ggtaatcttg gttctacgaa tgcattcaga gttcttggag ttccactcgg aataattgtc
     6301 cagttgcttg atatttctaa aggactgttt gcagttttgg tagtaactag attattttac
     6361 cctgacctgc cttttgaaaa ttacacacct tttgaagata taaccgtttt aaaaataatt
     6421 tccggtatca gtgctgttct tggacataca ttctctgttt ttgtaaaatt caaaggcgga
     6481 aagggtataa atactgcact tggaatgctt atctcacttg caccagttga agtgggagta
     6541 agcgttgggt tttttattgt aacactttta ttctcaggat atgtgtcact tggttctata
     6601 gttgcttcat cagtatttcc cgttactatg tttgtcaggg aaaacttttt caaagtagac
     6661 atttacggat acaacacctt aatatttttc agcataggaa tttcattgtt actgatctat
     6721 aatcacagag aaaatattaa aagactttta tacggaaacg aaaacagatt tgataagctt
     6781 tggctgatca ggatattcaa aatcaaagct cctttctctt aaaaataaaa tcatgaagat
     6841 cgctgtactt ggtgccggcg gctggggatc tgcccttgcc attcttctga atcaaaacgg
     6901 aaataaagtt actctctggg aatttaatcc aaaatatgct gagacattat ctctgcacag
     6961 agaaaatttt tattacctgc ctggaattaa aattccgaaa accattgata ttacaaatga
     7021 tctggaatat gctgtaaaag acaaatccct tgttgtaatt tccaccccga cacaatttat
     7081 cagaaatagt ctaagccgat taaaaaattt tgattttaaa gacagtcttt tagtcagtgt
     7141 ttcaaaagga attgagatct cttcaaattt tctggtttct gatatactaa cagatatttt
     7201 tggtgggata aaaagagaaa atattctttg tctgtcagga ccttcacatg ctgaagaagt
     7261 ttccagaaaa ttacctacgg ctgtagtctg ttcatcatcc gacaataata aatcaagaat
     7321 agtgcaaaaa gtattttcaa atagttattt cagagtctat tcgaatgatg atattattgg
     7381 aacagaagta tgcggcgcat taaagaatgt aattgcaatt gcggcaggaa tatctgacgg
     7441 taccggcttt ggtgataata caaaagctgc cattatgacc agaggtattc acgaaatcat
     7501 gaaacttggt ttaaaaatgg gagcaaagaa ggaaacattc tttggacttg cagggatagg
     7561 tgatctgata gtaacttgtg gttctgatca ttcacgtaac agaagagttg gcatacagct
     7621 gggtgaaggt aaaaagctaa aggacattct gtctgaatca aagatggtgg cagaaggtgt
     7681 cgctactaca aaggctgctt atgatctatc caaaaaactt aatattgaat tacctatcac
     7741 tcatcaggtt tatgacattt tatataaaaa taaaaaacct ataaaagcaa cagaactttt
     7801 aatgacaaga aatttaaaag aagaatttta atctacctaa aaaaaaagca ggtttaataa
     7861 aacccgcttt tataaattct caccgctgta aaactaaata atttttttaa aaatttatca
     7921 gagaaaaaaa ctttagaaca agctaaagaa ttttaatctc agacatgtgc tgacttcttt
     7981 tcttctttta aaatcctttc cagcctcatt gacttggttc taaatgtttt attaaagggt
     8041 gcaataatat aagctttaat aatgtaaggc cagaatccgc gctctttaag atgaggtgcg
     8101 atcagatggt caatgctttt gtgtgcttca ggtgtttcac tccagtggat acttgctctg
     8161 aaatgatgta ttgtatgaaa tccgttgttg aataacataa aattagttgt cggacttaca
     8221 aaatttctgg aatgattgta gtcagattct tcatcagcgt gtacatgctg aatgtaattg
     8281 aaaatcaaca cagagaaaag tgcaaactgt tgaggaatga taacatacag taaggctttt
     8341 tgccagtcta tcaaaagtgc aacgataata aacaagccaa gtgcaacata ctgaaaaatt
     8401 gataagaaaa atttttttct cttattgttc cacagaaatt taagataaac tacaatagga
     8461 ttctgctgaa aataactact gatggtcggg tagctgagaa gtgtgaaaat attatttttt
     8521 tcactgactc tgtatgtaat agtgaagtca ccgtcacgat tattgaattt atggtggttc
     8581 ttattgtgag taggagtcca tgcaaaaatt ggaaatccat aaaataatgt gatccagtaa
     8641 tctgtaatat gattcagtaa cttagatttc cacattggaa cgtgattatg attatgggca
     8701 atgactgcaa ttgttactgc aagataaagt gaaataatat aagtaataat atgaaatccg
     8761 aaattccatt gaaagaacag cattgcagta gttaaactaa gaaataagag ggtttttaaa
     8821 tcggctttgt attttaacat gatgttataa ttgaattttt taaagtattg ctgaagataa
     8881 ttttagatct gttctgatta aagaaggaag ataaaattta gattgatata aagtaatcaa
     8941 tatctgtgct taattcaatt acaatctact tattaaaaag aaaaataaat gatcataaaa
     9001 tacaaaaaga ttttgaaatg gtagtttgaa aataaaaaaa gcgggttcct ctcagacccg
     9061 ctttttaaaa atcaaatttc ttaacgaatt attttacaag agccattttc atgacttttg
     9121 tgaatccgtt tgtttctagt ttatagaaat aaacaccact tgaaagattt gaaccctgga
     9181 aattgataga gtgaaaacct gcagacatac ttccgtttac aagtgtagct acttctttac
     9241 ctgaattatc aaataccttt aaagttacaa aagcatcact ggggatctga tatttaattt
     9301 ttgttgatgg gttaaatgga tttggataat tctgacttag atcaaaatta ttcggtactc
     9361 cgattacaac ttcaccattc agattgtgat attcaaaatt accattataa tctatttgtt
     9421 ttaatctgta agtataaatt cctgaagtca gatttttatc attgaaagag taattttgtg
     9481 atacagaaga atttccatta ccagttacat atccaacttt atcccaggca ttagttcctg
     9541 ttttatatct ttctatttca aaaccggaat tatttatttc gcttgatgtt gaccagttca
     9601 gcataatatc attaccggaa ataccagcag taaaactgct tagctcaaca ggtaatgcac
     9661 cccatgtagt ttcagtagat ctaatagctg tagttgttct accaagtact aaaagctgca
     9721 aaacaggatt taaaacttcc gctgatgaat atgtagttcc accgggaacc tcaagagttg
     9781 ctgaagctga aataacgtca cctgaagaag ctcctgtaac tgtaacagta tcagatactc
     9841 caaatgcatt ggtacttact gtatattgac tcagagtacc ggttccgttt attgcaagtg
     9901 tgatacttgc tactgcaatt ggatttcctg cagtatctaa agtttctata taaaaatcat
     9961 caggatctga tccaggtttg atttcaagag ttgatacctg aatagtcgaa cctccgttta
    10021 aatttacatc agctataatt tcttttactc tgagaatgaa atcagcaact gtgacagtac
    10081 cgcctgtaat agctgttatt gaatccgggt caacgttatt tctgaaatgc cagattgcaa
    10141 gctgaacagc agatccttca tcattattgt ttgccaattt tcccggatat gtagttcttg
    10201 aaggatagta ggtattagta atataaattg cctgtggaat tgatgatgca gaatctttta
    10261 tagtatcacc taatgctata ggagtacaga catcaaggca ataaaaagga gtattaacaa
    10321 cgccatcaag agtcgcagga attacaccgg catatcctgc agcaacacct tcattacaat
    10381 gtgcaaatgt tacatttctt cctacaaact gagcattgaa agttgtgttt acaatagcac
    10441 ggctgtttcc gtattgtgaa tatgaatcac ttgcaattga tagaactaaa agcatgatta
    10501 aaaaagtaat ctttttcatt ttgggtttta gtgtttaata aaataattaa ttaattgtta
    10561 agagaataaa agaaataaac tggggagtct aaatcttttt gaagaattct aagaaaaata
    10621 tattgatttt aataagtgac ttttttattg gctattatac cccctttctt atatataata
    10681 agaattaata aataaattcc tgaaaattac tttggggtat aattttgata ggaatgtaaa
    10741 tatgtaaaaa ttgaattcaa aaataagcca attaatttaa aaaatcaatt actttaattt
    10801 tttgtgatgg aaaaatcaaa tatatcttgt cttaaatcaa tttggacttg tcaatatttt
    10861 ttagaactaa aatttgaaat taaaattatc tgcaaatcta agactttcat aattactaaa
    10921 taacttagtc taataaagaa aattttttga taaaataaaa acagccggct aaaccacatt
    10981 aaccgactgt aaacaaaaca aatgaaaaac actctatttt aatttagtta ttttactaat
    11041 gccattttca tgacctttac aaaacccggt gtttcgagcc tgtaaaaata aattccgctt
    11101 gatagacttg aagctttaaa gttgacagtg tgatatcctg ctgagacatt attattgaca
    11161 agtacagcaa cttcttttcc tgaattatca aatactctta tagatacaaa tccgtcattc
    11221 ggtaattcgt atttaatttt tgtttcagga ttgaatggat tcggatagtt ctggctcaat
    11281 tcaaatttag aaggtgttcc aatttctact tcattagtta aattgaaata ttcaaaatta
    11341 ccgttaaaat caatttgctt taatctgtaa ctatatctgc ctgaagttac atttttatct
    11401 gtgtaagtat attcggaaga atttaaagtt gttccatttc ctaagacatt tcctgcaact
    11461 ttccattcac ctgttgtaac agacattctc tcgatatcaa atcctgaatt attcagctca
    11521 gatgctgtga tccagtttag actaacatta ttattgttga cagatgaaga aaatgaagta
    11581 agctcaaccg gtaaagctcc ccagtctgaa taaactctta actcaccggg acccgggcat
    11641 gcaagtacaa ctttcggaca atcgttatta atatgtctga aaattctgcc ttttggcaaa
    11701 ataatattgt cagcagttgc tgtaatcgtt cctacacctg ttccgatcac ctggatcgga
    11761 tctgaaattc ctgaagcatc tgtatttaca gtataagtac ttagtgaacc ttctgtaatt
    11821 gttaactgaa ttccgttcac tgctacacct gaaccggcat cattttttgt ctcaataaag
    11881 aaaaagtccg gatcaaaatc gggaacgatc ttaattactt caggcactac actattattt
    11941 ccattaacat ctacatttgc tttaattgca tttgctctgt ttcttactgc tgaactagtt
    12001 attgaattca gatcaagtcc attcatataa tgccatatta cagcctgaat tgctccagat
    12061 tcatttgcaa gattgcttaa ctgtcccgga cctgttgcag ccggataata attgtaaaga
    12121 atgtagcaaa cccgtgaagg taaaattcct gttgagtcat ctacataatc aaaatttgga
    12181 agacaacttg aaggtgcttt aatgatgtca atgctgtaaa agcagacatc cggtccggaa
    12241 tttggattgt atatttttcc ataaaacaat ccaacagttc ttgtttctga ttgatttgta
    12301 ataggatcta taaaagtaat atttcgcttt ggcgaaatac cttctactga agcgggtgta
    12361 agttgagcta ttgaaattcc attagaaata gaaatcaata aagcaacaag aagaagaatt
    12421 ttcttcatgg gggtctctcc tttcaagaaa gaggaaaatc aattcttgta ttgacctttt
    12481 gggatggtca atttaatcaa atatttggga tatcagatta aaaaaaattg attttcctta
    12541 atttaatata ttgtgtaaaa aatagttgtt aaaaataaaa tatttaaatt tagttatcaa
    12601 ataaatttaa gtcattttgt gaagttaata aaaacaataa tccagttaaa tatttgaaaa
    12661 atgctatttt tgattgttat tgataataag tttagaatat ttaatatcta actgtgaagt
    12721 ctgacaaagg atttttatta aaaaaaaagg cggcatatag ccgcctttac gatacaaatt
    12781 gaaattattt aattaaagac attttcatga ctttagtaaa gtttcctgct gaaagtttgt
    12841 agaaataaac accactggtt agtccagttg cattaaaatt aattgtatgg aatccggcat
    12901 tcaaatttcc attcactaat tctaaaactt ctttaccgct gatatcaaaa attttcaatg
    12961 atacagcacc gtccactggg atctggaaac tgatctttgt agatggattg aaagggttag
    13021 gatagttctg attcagacta tattcatccg gtgcagcgat cactacttca ttactgagat
    13081 tgaaatattc aaaattacca ttgaaatcaa cctgtttcag tctgtagctg tattgacctg
    13141 cactcagatc tttatctgta aatgaatagc ttcgggtttc gttagttgta ccgttaccgg
    13201 ttacatttcc aaccttagac catgtttcgt ttgtcgatac ttttctttca atatcaaaac
    13261 ctgagttatt tatttctgaa gatgtagacc agtttagagt aacatttctt gaattaataa
    13321 ctgaagtaaa tgaagataac tcaacaggta atgcacccca atcaaaagtt gcttcttttt
    13381 ctccgatagc cggcgcagct ataacaagtc tctgcttatt tggacatgca tatgtaactc
    13441 cctgcgggat catgcacatc gcagtagcag tgataatacc tgttcctgta cctaatacag
    13501 ttatcgctgg tgattcacca tttggtaatg tagtatttac attgtttgtg cttaacgaac
    13561 cttcggaaat tgaaagctga atattgttaa ttgctattgg gtcaccattc tgatcaaggg
    13621 ttttgatata aatctcgtca ataccaatac ctggttcgat agaaaatgta gtaacaatat
    13681 ttgttatcac cccgtttgca tctgcatctg caattatctc aagtgttctg actttgatag
    13741 tattgtcagt aattgtgttt gcatccattc cgtcagaaaa atgccagatc gcacattgag
    13801 ttgatgcgac ttcatcattc agatttggta attctcctac cggatcagga ttgtaaggaa
    13861 ggtaattgtt taagacataa acaatctttg agcttgatac tgcagaatct ttatgacatt
    13921 catctggaaa acttaatgtc cttctgagat caatgcagta aaattttgta gcgttattat
    13981 cgacactacc gttcatttga ccggcaaaca cttgtcttga tgcatttgta taagggtcga
    14041 taaatgttac acttttacca gcccctgtgc ctgttataat tgcatcactt agtccatatt
    14101 gagaatatga aattgaagtg aaaactaata caacaagaat tactaatgaa aaaataacct
    14161 ttttcatttc tttctccttt cgtttatttt ggttttttaa aatcagaaaa attctgtgct
    14221 aatatttttg ggaaatatta attacagctt tggggagact gtaaaaaaca gaattcttct
    14281 gaaaatctat gtttaaagtt ttttaataaa tttttttttt aaaattttga aatataaatt
    14341 tcgtttaaaa taatgttgca aaaataaagt aattaatttt tataaacaaa taaatttatg
    14401 ccgatttgtg aagcagtaaa taatgttatt aatatagata acccgattaa ggggattaat
    14461 ttttaattag ttaatgaaaa tccgaatata actaaaaatt ttgtgaagta atctcccgga
    14521 tcatttacaa tcaattactc aaatatttgt aaaaagtatt tatttaaaaa agaacttatt
    14581 attacattag taaagaaatt tttattacac cggagaaaat tcaggttttt gtatttagaa
    14641 ttagaatcgt aaaaaagcaa aaaatcttct taattatttt tcgcctttca tatgtttaat
    14701 tttcttgttc aa
//
LOCUS       DAORVQ010000077        14685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1836_length_14685_cov_5.436500, whole genome shotgun sequence.
ACCESSION   DAORVQ010000077 DAORVQ010000000
VERSION     DAORVQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_14685_cov_5.436500"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLX80_07800"
     CDS             <1..304
                     /locus_tag="PLX80_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HQY20726.1"
                     /translation="TKIRTSNYIISRTKDFKQLDNILRSQLKLLTVVSKVRHIFIKNN
                     IRIHLDRVKKLGDFIEIEIIYKEIKSAKKQMKELIEFLELNKNDFIKHSYSDMLIK"
     gene            309..1130
                     /gene="panB"
                     /locus_tag="PLX80_07805"
     CDS             309..1130
                     /gene="panB"
                     /locus_tag="PLX80_07805"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQY20727.1"
                     /translation="MSSQKKETEHHITTKVLFAMKQRKEKIAMLTAYDFLTAKFLDAA
                     GIDVILVGDSLGNVIQGNETTLPVTLEEMIYHTKIVKRAVKNAIVVADMTFMSYQVGV
                     KEAITNCGRMMKETGCDAVKMEGGDYIAETVRRVVEIGIPVMGHLGLTPQSINKFGSY
                     KTRGTEKEEADKIYKDSLLLEKAGCFAIVLEKIPAKLGTKISKALKIPTIGIGAGPSC
                     DGQVLVTHDMLGFIEEFKPRFVRRYANMAVDSKEAFKKYIKDVKSEKFPTLKESY"
     gene            complement(1190..3577)
                     /locus_tag="PLX80_07810"
     CDS             complement(1190..3577)
                     /locus_tag="PLX80_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQY20728.1"
                     /translation="MKLTNWFKLILSIAVFSLLTIVNVNSKDIQDSSPKHSKMKIFIN
                     GNIDMEKINSQGLFFDHAEMQDGSMETWLSEDEIILLKKSGIPYQILIEDWDEYYSTI
                     AKMTPSEVRDAIQLSVLEYNVSHSIYGTMGGYLTFSEVVSKLDSMRIEYPGLISQKFS
                     IGQTVENREMWTVRVSNSPNAPTGRPEVWFHSLIHAREPMSMEQNIYFMYWLFENYNI
                     DPIATYILNNREIYFTPVFNPDGYEYNRSTDPNGGGFWRKNRKQFGGNTFGVDLNRNY
                     GIYQFWNSANNGSSTDSTSDTYRGKSPFSEPETRNAMNFVNSRNFRGVLSYHTYGNYL
                     IRPWGHVDAPSPDEKIFQNISQDMVLNNHYTLGRSNQTVNYGVRGVTDDWYYNDSGHI
                     KAFAMTPEVGTGNDGFWPLQSRILPLAKQNVSQNIYFTMFSGPFMNTNSVALNSEYYL
                     PGETGTLKVILKNKGLENASNIRVELVSEHPLITVLTNPMTYSDYNSFREDSVSFNFS
                     VAPNATTISAYKTKLKIFQNNTDLVYTKDINIVIGSGNVLLLDSAENGLGKWTVSGGF
                     GTTTSQFHSSSRSFSDSPTGLYSNSSIRTMTLQTPLNITANPVVILSFWYKHTIDTLD
                     NAYVDVSSDGGTSWRSEKFYNKTVNNWTKEIIDITELAGGVNLKIRFSLVTNGSVTSD
                     GIYIDDIRLAGYNSITTGITSENEIPLKYDLSQNYPNPFNPSTSISYQILQGGNVSLK
                     VYDINGKFISELVNENKPAGNYEVPFSGEGLSSGIYFYRIETGNFSQTKRMMLIK"
     gene            complement(3648..4637)
                     /gene="glk"
                     /locus_tag="PLX80_07815"
     CDS             complement(3648..4637)
                     /gene="glk"
                     /locus_tag="PLX80_07815"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HQY20729.1"
                     /translation="MEKIILAGDAGATKTELAVYKCSNGVLNVISSLKFESKAYSSLI
                     EIIKLFLNSVGLKPDAACIGVPGPVIEGKVVSTNIPWQLDEKELSDKLNIPVFKLAND
                     LEAAATAIPEMDSKYLAKIYKGKDAAKDGNKMVIAPGTGLGQAILIYNDGKYNVISNE
                     GGHADFAPTSDIEIELLKFLKAKYGHVSIERVISGIGILNIYEFLLTKDEFKDSENFF
                     DDSSIRDIAAKISEEGIYGKSRICEKVLEIFVSSLAELCGSKVLSMNATGGVYLCGGI
                     PPKILKILFTENFAGSYLNKGRLSYYIEPVPVYVVTDDSAGLKGAALLALKNL"
     gene            complement(4644..5381)
                     /gene="pgl"
                     /locus_tag="PLX80_07820"
     CDS             complement(4644..5381)
                     /gene="pgl"
                     /locus_tag="PLX80_07820"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQY20730.1"
                     /translation="MFESKESLSVNLADDFIHICSDILKEKEFLNIALSGGNTPKLFF
                     GKLLNKRSVFNDWDRLRFFWVDERCVPANDIESNYGEAKRLLFDRMKSENDEVLIPDE
                     NIFRINGENDPADESIRYAELLMKELPFENSFPVFDLMLLGIGDDGHTASIFPGMLEL
                     MDSDKYCAAVQHPVTGQYRVTLTGKVINNSKKIIFMITGKDKANVIFEIINEKGNFEK
                     YPSYYVKPIDGEILIYIDNDAAEFLQK"
     gene            complement(5458..6987)
                     /gene="zwf"
                     /locus_tag="PLX80_07825"
     CDS             complement(5458..6987)
                     /gene="zwf"
                     /locus_tag="PLX80_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQY20731.1"
                     /translation="MPDPFTLIIFGASGDLTERMLIPSLYHLFSENELPENFSVAGFS
                     RKEMTNESFRENMKAGVIEHYGPDNFNEDIWKKFEKNLYYFIADYKDSGSYKKLLSEI
                     EKIENEKKSGGNRLIYMAVPPSSLSEIITNTGESGLNKEVDKNSWTRIILEKPFGHDL
                     ESAMKLNNIVSKYFSEKQIYRIDHYLGKESVQNILVTRFANGIFEPLWNRNYINHIEI
                     TSSENIGIESRGGYYDNSGALRDMVQNHLLQLLGYIAMEPPSTFDSNSIRDESLKVFQ
                     SVRPVNESEVDKVVIRGQYTGSKIRGEKVNGYREEEGVEENSRTETFVALKLFIDNWR
                     WGGVPFYIRTGKRLPTRVTEIVIHFKATPHHLFKSESDIAESYNMLVIRIQPDEGLLL
                     KFAMKVPGSEFKVQNVNMDFHYTDLKDLDLNTAYEKLLLDCMLGDSTLFIRADSVEAC
                     WEIIQPILNAWNNNPDIIVYGYPAGTWGPECADDLIAGKDFQWRYPCKNLANDDNYCE
                     L"
     gene            complement(7241..8137)
                     /gene="gnd"
                     /locus_tag="PLX80_07830"
     CDS             complement(7241..8137)
                     /gene="gnd"
                     /locus_tag="PLX80_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HQY20732.1"
                     /translation="MKIGFIGLGKMGAFMVERLLNDGHEVVVYNRTPEKTREIEMKGA
                     IASYSLEELVEKLPSPKIVWMMIPSGDPVQKTIGELIPLLNKEDIIIDGGNSYYKDTV
                     RRSAELEKVGINYLDAGTSGGIWGLKLGYCVMVGGEKNIFEQCEPVFKTLAPENGYKY
                     IGKAGAGHYVKMVHNGIEYGMMQAYAEGFELMHKSDYNIDLHGVAELWDQGSVVRSWL
                     LQLLANALKDDKDLSSLKDYVEDSGEGRWTVLDGIEKSVPLHVITDSLFARFRSRQDE
                     SYGAKILSALRNEFGGHAVQKK"
     gene            complement(8160..9890)
                     /locus_tag="PLX80_07835"
     CDS             complement(8160..9890)
                     /locus_tag="PLX80_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20733.1"
                     /translation="MNITKNDFSFGRYKSDIKKKIEKIETSDIPERIFNKDFTVWKDL
                     PEFEQLIKNRLGWLMLPITMQEKTDEIDSFVSEIRKSGFEYAVVMGMGGSSMCPEVCR
                     DTFGVRKGYLDLFVLDSTDPQSVTDIENSIDISKTLFIVSSKSGSTIEVDSFLRYFFA
                     KTEEIFSDDAGSRFIAVTDPGTYLDSLSAELGFRKTFVNPADIGGRYSALSFFGLVPA
                     ALIGVDIKRFLKNAEVVMNMCFENSFFKNPGFSTGMIAAYLSEFGVDKLTFVLSDKIK
                     SFGYWVEQLIAESTGKEGKGILPIEGEITGKANSYNRDRFFVNIYLKKDKNKKKVKAL
                     AKEKYPVLNIEIDELYDLGGLFYLWEFVTAVIGYAMDINPFDEPNVKESKDNTSKVLD
                     YYEENKKLQEQTAVSGNKKIKLYTGKESDTLNLLSFKKGKAKISEVIKYFTDQFAVGD
                     YFAVMAYLNRSEKADSPLQELREILRSKFKSATTVGFGPRFLHSTGQFHKGGPDKGMF
                     IQIVCDDKTDIEIPGKDFSFSILKQAQSEGDFESLQKHDRRVLKFDIGKDINKGLKEI
                     IKEFKRALKK"
     gene            complement(10082..12085)
                     /gene="tkt"
                     /locus_tag="PLX80_07840"
     CDS             complement(10082..12085)
                     /gene="tkt"
                     /locus_tag="PLX80_07840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQY20734.1"
                     /translation="MTKIDELCINTIRLLSADAVQKANSGHPGMPMGAAAMAFSLWTN
                     EMNYDPSDPDWPGRDRFILSAGHGSMLLYSMLYLTGYELTLDDIKNFRQLDSKTPGHP
                     ESHLTKAVETTTGPLGQGFANGVGFAIAQKYLSAKYDRPGFNIFDYNIYAIVSDGDLM
                     EGISSEAASLAGHLKLGNMIYLYDDNHISIEGNTEIAFTEDTAKRFEAFGWQVIEVSD
                     GNDPDKISEAVKKAKQEKDKPSLIKIRTHIGFGSPNKADSAGSHGSPLGEDELRLTKE
                     NLGFDPDKSFYVPDEVLKFFRDKGENGKKKKDEWEKLYSEYSGKFPDESKGIGRLMKG
                     EFIEGWEKAFPVFTTDEGKMATRSASGKTINAIAEYFPQMIGGSADLEPSNNTFLKKY
                     KSFQPGNYDGRNLHFGVREHAMGAVLSGITLTLPMIAYGATFLIFSEYMRAPIRLAAI
                     MGIRPIYVFTHDSIALGEDGTTHQPIEQLISLRSIPNLTVIRPADANETSQAWKTALE
                     IKSGPVALVLTRQNLPIIDQSEFGSAENLNKGAYILSDCNVEPEILLIATGSEVSLAM
                     EVQIALQSENISSRVISMPSWELFEKQTDEYKEMIIPKKIKKRISIEAGSKLGWHKYT
                     GDNGLNIGIDEFGKSAPGDILMKEYGFDKDKIIDKLKKHFQIK"
     gene            complement(12275..13600)
                     /locus_tag="PLX80_07845"
     CDS             complement(12275..13600)
                     /locus_tag="PLX80_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20735.1"
                     /translation="MKKFIIITVFILQSGLSYSIQGWVEYPEITSNRLRSIYFIDSNT
                     GFVCGNNGALFKTTDGAANWFPLSNPSNLDFQNIYFIDDQRGLLTTVPEGSEAGYWFI
                     VYMTTDQGASWFPLKQFGNFISDFYNDGNEFFLSFEGWYGFETAGDISYSQVNAVNFN
                     SLPFFEYFRNSRVLSVCKSNDKIWISASFGDDAGTDVNRLGYTSNSGSSWNIIYNDSA
                     VLWNADPSHNRFYKIRFLNDSIGYLASMLGLMKTVNGGNDWSFIDTTFTKNIRHHFFT
                     TADTGWVFKQNNIFKTTDGGNNFDLQFSYNSNFTQPFFLNSLTGYILCANGRILKTIT
                     GGLTGIYSNTNISLPEIFSLSQNYPNPFNPSTLIRYSIPSDVRSQTSDVKLIVYNNLG
                     KEIMTLVNEKQNAGSYAVDFNGDGLPSGVYFYRLVAGEFEETKRMILLK"
     gene            complement(13612..>14685)
                     /locus_tag="PLX80_07850"
     CDS             complement(13612..>14685)
                     /locus_tag="PLX80_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20736.1"
                     /translation="STRGGILKTSNKGEHWNFIYYDSSQMLENIYFVNSLTGWFPKAN
                     NINKTTDGGNSWNVQNLNPSGEVGEMFFMNPSTGWISVKSNGIYVEKTTNGGENWISQ
                     RVLDETWYRIFQVYFSNEQNGTLIGNNITSITSDGGLNWQSYTNNYSFSVSCNDDLGN
                     LYACSFNKVYKSIDHGLSWQLKSNPINGNGFFSFKMVNSNTGYLGSVSGLLFKTSNGG
                     VDWIRQNISALQIRSISAIDTNCWVVGDGGMILSTAGSREIVSDIKLNNIIIQEFFLR
                     QNYPNPFNPNTTINYQLPVTKHVSLKVYDVIGKLVASLVNEKQNAGSYTVDFNGDGLP
                     SGVYFYKLEAGEFTETKRMILLK"
BASE COUNT         4494 a         2896 c         2298 g         4997 t
ORIGIN      
        1 gacaaaaata agaacatcaa attatattat ttcccgaacc aaagatttta aacagcttga
       61 taatatattg agaagccagc taaaattgtt aacggtagtt tcaaaagtga ggcatatatt
      121 cattaaaaat aatataagaa tacatcttga cagggtaaaa aagctgggtg attttattga
      181 gatcgaaatt atttataaag aaattaagag cgctaagaaa caaatgaaag aattaattga
      241 atttcttgaa ctgaataaaa atgattttat aaaacattct tattcggata tgttaattaa
      301 ataaatatat gtcatcacag aaaaaagaaa ctgaacatca cattacgaca aaagttctgt
      361 tcgcaatgaa gcagagaaaa gaaaagatcg caatgctgac agcttatgat tttctcactg
      421 caaaatttct tgatgcagca gggatcgacg ttatacttgt cggtgacagt cttggtaatg
      481 taattcaggg aaatgaaact actttacctg ttacacttga ggaaatgatc tatcacacaa
      541 agattgtaaa gagagcagtt aagaatgcaa tagttgttgc tgacatgaca ttcatgagct
      601 atcaggtcgg cgtaaaagaa gctatcacaa actgcgggag gatgatgaaa gaaaccggat
      661 gcgatgctgt gaaaatggaa ggcggagatt atattgccga gactgtaaga cgtgtagtgg
      721 aaattgggat tcctgttatg ggtcatcttg gacttactcc acagtctata aataaattcg
      781 gaagttataa aacacgcggc acagaaaaag aagaagctga taaaatatat aaagattcac
      841 ttttacttga gaaagcagga tgctttgcaa ttgtccttga gaagatccct gctaagctcg
      901 gaactaaaat ttccaaagct ttaaaaatac caaccatcgg aattggtgca ggaccgtcct
      961 gtgacggaca agtacttgta actcacgaca tgctcggatt cattgaagaa ttcaaaccaa
     1021 gatttgtcag aagatatgcc aatatggctg tcgattccaa agaagctttt aaaaaatata
     1081 tcaaagatgt taaaagtgaa aagtttccga ctctgaaaga aagctactaa tcaattttaa
     1141 ttttaaatat taaaatataa attattattt ttttataata atttatatat tatttaatga
     1201 gcatcatcct tttggtctgt gaaaaatttc cggtctctat tctataaaaa tatattccac
     1261 ttgaaagacc ttctccgctg aacggtactt cgtaatttcc tgcaggttta ttttcattta
     1321 caagttctga aataaactta ccattgatat catatacttt caaactcacg tttccacctt
     1381 gcaggatctg ataacttatt gatgtagatg ggttgaaagg gtttggataa ttctgactaa
     1441 gatcatactt taaaggaatt tcattctctg atgtaattcc tgttgttatg gaattgtaac
     1501 cagctaatct gatatcatca atataaatac cgtctgatgt gacagatcca ttagttacta
     1561 atgaaaatct gatcttaaga tttacacctc ctgccagctc agtaatatca atgatttctt
     1621 ttgtccagtt atttacagtt ttattataaa atttttcaga tctccatgaa gtgccgccat
     1681 cacttgaaac atccacataa gcattgtcaa gtgtatcaat tgtatgctta taccagaaac
     1741 tgagaatcac aaccggattt gcagttatat ttaaaggtgt ttgcagagtc atagttctga
     1801 tactggaatt cgaataaagt cctgtagggc tgtctgaaaa agatcttgat gatgaatgga
     1861 attgagaagt tgtagttcca aatccacctg atactgtcca ttttccaaga ccgttttctg
     1921 cactgtctaa aagcaaaaca ttaccactcc caattactat gttaatatct tttgtataga
     1981 ccagatcagt attgttttga aaaattttta atttggtttt ataagcagaa attgtcgttg
     2041 catttggtgc cactgaaaaa ttaaacgaaa cgctgtcctc tctgaaggaa ttgtaatcag
     2101 agtatgtcat aggatttgtc agaacagtta ttaaaggatg ctcagaaact aattctactc
     2161 ggatatttga agcattttcc agtcctttat ttttcaaaat aactttcaaa gttcctgttt
     2221 cacccggtaa ataatattca ctattcagag cgactgaatt agtattcata aaaggaccgc
     2281 tgaacattgt aaaataaata ttctgcgata cgttttgctt tgcaagagga aggatccttg
     2341 actgcagcgg ccagaagccg tcatttcctg ttcctacttc aggtgtcatt gcaaatgctt
     2401 ttatatgtcc gctgtcatta taataccagt catcagtcac acctcttaca ccataattaa
     2461 ccgtttggtt agatctgccg agagtgtaat gattgttgag taccatgtcc tgagatatat
     2521 tctgaaaaat cttttcatca ggacttggcg catccacatg accccatggt ctgatcaggt
     2581 aatttccata agtatgatag ctgagtacgc ctctgaaatt tctggaatta acaaagttca
     2641 ttgcattgcg tgtctcaggt tctgagaaag gcgacttgcc tctgtatgta tcgcttgtag
     2701 aatctgtcga tgaaccgttg ttagctgaat tccagaattg ataaattccg tagtttctgt
     2761 ttagatccac accgaatgta tttccgccga actgctttct gttttttctc caaaagccgc
     2821 cgccgtttgg atctgtagat ctattatact cataaccgtc cggattaaaa acaggagtaa
     2881 aataaatttc tctgttattt aaaatgtaag tagcaatagg atcaatatta taattttcaa
     2941 aaagccagta cataaaataa atattctgtt ccatgctcat cggctcacgc gcatgaatca
     3001 gtgaatgaaa ccagacttca ggtctgccgg tcggagcatt tgggctattg gaaacccgca
     3061 ctgtccacat ctcgcggttt tctactgtct gaccaataga gaatttctgg gagatcagtc
     3121 cgggatattc tattctcatc gaatctaatt tagagacgac ttcgctgaaa gtcaagtaac
     3181 ctcccattgt tccgtatatt gaatgactga cattatactc gagtacagaa agctgtatag
     3241 catctctgac ttccgaggga gtcatttttg caatagttga ataatattca tcccagtctt
     3301 caataagtat ctgatatggt attcctgatt ttttaagcaa aattatttca tcttctgaaa
     3361 gccatgtttc cattgagcca tcctgcattt ccgcatgatc aaaaaataat ccctgcgaat
     3421 ttattttttc catatcaatg ttaccattga taaaaatttt catttttgaa tgtttcggtg
     3481 aactgtcctg aatatctttt gaatttacat ttacaattgt cagtaatgaa aacactgcaa
     3541 ttgaaagaat taatttgaac caatttgtta atttcatttt ttcttatttt ttaaaaatat
     3601 ttataattta ttaataattt tttgttttcc cgtattatta tttacagtta aagattcttt
     3661 aatgccagca atgctgcacc tttcaatccc gctgagtcat ctgtaacgac atagaccggt
     3721 acaggttcaa tataataact aagtcttcct ttattaagat atgatcctgc aaaattttct
     3781 gtaaataaaa ttttcaggat tttcggaggt ataccgccgc acagataaac acctccggta
     3841 gcattcatac tcagaacctt actgccgcag agctctgcaa gagacgaaac aaatatctca
     3901 agaacttttt cgcatatcct gcttttaccg tagattccct cttctgagat ctttgctgca
     3961 atgtctctga tactgctgtc atcaaaaaaa ttttcagaat ctttgaattc atctttagtc
     4021 agaagaaact catatatatt cagtatccca attcctgaga ttactctttc tatactaaca
     4081 tgcccatact tagctttcag aaatttaaga agttcaattt caatatctga agttggagca
     4141 aaatcggcat gaccgccttc atttgaaatg acgttatatt taccgtcatt ataaataaga
     4201 attgcctgac caagtcctgt ccccggtgcg atgaccattt tatttccgtc ctttgccgca
     4261 tcttttcctt tataaatttt cgcaagatat tttgaatcca tttcaggaat tgcagttgct
     4321 gcagcttcta ggtcatttgc taatttaaat acaggaatat tcaatttatc tgaaagctct
     4381 ttttcatcaa gttgccatgg gatatttgtt gaaactactt ttccctctat gacaggtccg
     4441 ggaactccga tacatgctgc atcaggtttc aatcccaccg agtttagaaa tagtttaatt
     4501 atttctatca gagaactgta agctttactc tcaaatttta atgaacttat aacattcaaa
     4561 acaccattac tgcatttata aactgcaagc tctgtcttgg tcgcaccggc atctcctgct
     4621 aaaattattt tttccatttg tatttatttt tgtaaaaatt cagcagcatc attatcaata
     4681 taaattaata tctctccatc aattggctta acataatagg agggatactt ttcgaagtta
     4741 cctttttcat ttataatttc aaaaattaca tttgctttat ctttgcctgt tatcatgaaa
     4801 attatttttt tcgaattatt aattacttta cctgtaagag taactctgta ctgtcctgtc
     4861 acaggatgtt gtactgctgc acaatattta tccgaatcca tcaattcaag catcccggga
     4921 aaaatagaag ctgtatgtcc gtcatcacca attccaagaa gcatcagatc gaatacagga
     4981 aatgaatttt caaaaggaag ttctttcatc aataattcag cataccgaat tgactcatct
     5041 gcaggatcat tctccccatt tattctgaaa atattttcat caggtatcag gacttcatca
     5101 ttttcagatt tcattctatc aaataataat ctcttcgctt ctccataatt actttcaata
     5161 tcatttgcag gtacacatct ttcatccacc caaaaaaacc ttaacctgtc ccagtcatta
     5221 aatactgatc ttttattcaa tagcttacca aaaaaaagtt ttggggtatt tcctcctgat
     5281 aaagctatgt tcaaaaattc tttttctttt aaaatgtcag aacaaatatg aataaaatca
     5341 tctgctaaat ttacagagag tgattcttta ctttcaaata tttttaaatt ttcttttata
     5401 ataaaaattt attttaacaa tttagtagag attatttcag aatagaagta tttgtattta
     5461 cagttcacaa taattatcat cattagcaag gtttttgcaa gggtacctcc attgaaaatc
     5521 ttttcccgcg atcagatcat ccgcacattc cggaccccat gtacctgcag gatagccata
     5581 aacaataatg tccggattat tattccatgc attaagtatc ggctgaatga tttcccagca
     5641 cgcttccact gaatctgctc ttataaataa tgtagaatcg ccaagcatac aatcgagcaa
     5701 aagtttttca taagcagtat tcagatccag atctttcaga tcagtataat gaaaatccat
     5761 attcacattc tgcactttga attcagatcc tggaaccttc atagcaaatt tcaaaagcaa
     5821 cccttcgtca ggctgaatcc tgatcacgag catattatag gactcagcaa tatcactttc
     5881 acttttaaaa agatgatgcg gagtggcttt gaaatggatc acaatctctg tcacgcgtgt
     5941 aggaagtctt tttcctgtcc ttatataaaa cggaactcct ccccagcgcc agttatctat
     6001 aaataatttt agtgcaacaa atgtctcggt tcgtgaattc tcttccactc cttcttcttc
     6061 ccggtatccg ttgacttttt cacctcttat tttagatccg gtatattgcc ctctgatcac
     6121 aactttgtcg acttcacttt catttaccgg tctgacagac tgaaatactt taagagattc
     6181 gtcacggata gaatttgaat caaaagtcga aggcggttcc attgcaatat atccgagaag
     6241 ctgaagcaga tgattctgca ccatgtccct caatgctcct gagttatcat agtatccgcc
     6301 cctgctttct attccgatat tttccgaaga agtgatctca atatgattaa tataattcct
     6361 gttccagaga ggctcaaaga taccgtttgc aaatcttgtt acgagaatat tctgtacaga
     6421 ttcctttcca agataatgat cgatcctgta tatctgtttt tctgaaaaat atttactgac
     6481 gatattattt aatttcatag cagattcaag gtcatgtcca aatggttttt ccagaattat
     6541 tcttgtccat gaatttttat caacttcctt attgaggccg ctctcccctg tattcgtgat
     6601 tatttcagaa agtgacgaag gcggtactgc catatatatc aatctgttgc ctcccgactt
     6661 tttctcattt tctatttttt cgatttctga taataatttt ttatatgaac ctgagtcttt
     6721 ataatccgct ataaaatagt aaaggttttt ttcaaatttt ttccagatat cttcattgaa
     6781 attatccggg ccgtaatgtt cgatcacacc ggctttcata ttttctctga aagattcatt
     6841 cgtcatttcc tttcttgaaa aacctgccac tgaaaaattt tccggcagct cattttctga
     6901 aaataaatga tacaaagaag gaatcagcat tctttcagtt aggtctccgc tggctccgaa
     6961 tattatcaga gtaaatggat ccggcattct gttgttataa ctgggtttaa ctgtatattc
     7021 taaactcaat ttgtaagttt tgttaaagga tatttatatg taaaaaaaga ataatccgtt
     7081 gtatgcagtt gctggtgttt caaaaagttt tgatacagat tttgtatttt tatattcaac
     7141 aagtaatttt tttagttaca aacaattaaa gtcactgtga actcttctca gattgttgtt
     7201 gatctctaaa aatttaaact gttgaaaaaa taatctctct ttattttttc tgaactgcat
     7261 gtccgccgaa ttcatttctc aaagcagaaa gtatctttgc tccataagac tcatcctgtc
     7321 tcgaacggaa tcttgcaaac aatgaatcag tgattacatg aagaggtacg cttttttcaa
     7381 tgccgtcaag tactgtccat ctgccttcac ctgaatcttc gacataatct ttcagcgaac
     7441 tgagatcttt atcatccttc aatgcatttg ccagcaactg taaaagccac gatctcacga
     7501 cacttccctg atcccacagc tctgccactc catgaagatc gatattataa tccgacttat
     7561 gcatcagttc aaatccctcc gcatatgcct gcatcatacc atattcaatt ccgttatgaa
     7621 ccattttcac ataatgacct gcacctgctt tgccaatata tttatatcca ttctcaggtg
     7681 cgagtgtttt aaaaacaggt tcgcattgtt caaaaatatt tttctcacca ccgaccatta
     7741 cgcagtatcc aagtttaagt ccccatatac cgccgcttgt acccgcatct aaataattta
     7801 ttccaacttt ttcaagctca gcagatcttc tgacagtatc cttataatag gaatttccgc
     7861 cgtcgatgat tatgtcttct ttatttagta atggaataag ctccccgatc gtcttctgaa
     7921 caggatctcc tgatggaatc atcatccata ctatttttgg agatggtaat ttttcaacta
     7981 attcctcaag tgaataagat gcaatagctc ctttcatttc aatctctcta gtcttctcag
     8041 gagttctgtt atagacgacc acttcatgtc cgtcattgag aagtctttcc accatgaaag
     8101 ctcccatctt tccaagtccg ataaatccta ttttcattta tgctttatta atttattatt
     8161 tattttttta atgctctttt gaattctttt ataatttctt ttagaccttt gttaatatct
     8221 tttccgatat cgaattttaa aactcttctg tcatgctttt gtaaagattc aaaatcccct
     8281 tctgactgtg cctgctttaa aatactaaag ctgaaatcct ttccgggtat ttcaatgtct
     8341 gttttatcat cacatacgat ctgtataaac atgcctttat ccggtccgcc tttgtgaaac
     8401 tgtccggtcg aatgcaagaa tctcggtccg aaacctactg ttgtcgcaga tttgaatttt
     8461 gatctcaaaa tttctctaag ctcctgaagc ggtgaatctg ctttttcaga tctgttcaga
     8521 tatgccatga ctgcaaaata atctcctaca gcaaactgat ccgtaaaata ttttattact
     8581 tcagaaattt tcgctttacc ttttttgaat gaaagtaaat tcagtgtatc actttcttta
     8641 cctgtataaa gttttatttt tttatttccg gaaacggctg tctgctcctg aagtttttta
     8701 ttctcttcat aataatccaa tactttcgaa gtattatctt tactctcttt tacatttggt
     8761 tcgtcaaacg gatttatgtc cattgcgtag cctattactg ctgttacaaa ttcccataaa
     8821 taaaaaagtc cgccaaggtc ataaagttca tctatctcaa tatttaaaac cggatatttc
     8881 tccttagcca gagctttaac cttctttttg tttttatctt ttttaagata aatatttaca
     8941 aagaacctgt ccctgttgta tgaatttgct ttgccagtaa tttctccttc tattggcaag
     9001 atacctttgc cttcttttcc cgtactctct gcaattaatt gctctaccca atatccaaat
     9061 gatttgattt tatccgataa aacaaacgta agtttatcca caccaaattc tgataaataa
     9121 gctgcaatca tccctgttga aaaacccggg ttcttaaaaa atgaattttc gaaacacatg
     9181 ttcataacaa cttctgcatt tttcaaaaac ctttttatgt caactccaat cagcgctgcc
     9241 ggcacaagtc caaaaaatga aagtgcagaa tatctgccgc caatgtctgc aggatttaca
     9301 aatgtctttc ggaaaccgag ttcggcagaa agtgaatcaa gatacgtgcc cggatcagtg
     9361 actgcaataa atctgctgcc ggcatcatca gaaaatatct cttcagtttt tgcaaaaaaa
     9421 tatctgagga atgaatcgac ttcgatagtg gatccggact tgcttgatac tatgaataat
     9481 gtttttgaaa tgtctataga attttcaata tcagttacag actgcgggtc agtgctgtcg
     9541 agtacgaata aatccagata tccttttctt actccgaatg tatctctgca aacttccgga
     9601 cacatactgc tgccgcccat tcccattact actgcatatt cgaatccgct ctttctgatc
     9661 tccgatacaa acgaatctat ctcatcagtt ttctcctgca tcgtaatagg aagcatcagc
     9721 catcctaatc tgtttttaat aagctgttca aattcgggta aatctttcca tacagtaaaa
     9781 tccttattaa atatcctttc cgggatgtcg gaagtttcta ttttttctat tttctttttt
     9841 atgtcagact tatatctgcc aaagctgaaa tcattttttg ttatattcat attctgaatc
     9901 tgtaaattac tttatttaat aaaaattaat tttatgtgaa aatgtttttt cagtttatcc
     9961 gaaaaattgt tcttgttccc gcagagtcac gcgttcttcg gactctgcgg atactaattt
    10021 attaatacct gttccagcag agtctcgtga tcttcatact ttgcggatac tgatataaag
    10081 tttattttat ctgaaaatgc ttttttaatt tatcaatgat cttatcttta tcaaagccat
    10141 actccttcat taaaatatct ccgggtgctg acttaccgaa ttcatcgata ccgatgttca
    10201 aaccattatc acctgtatat ttatgccagc ccagtttaga tccggcttca attgaaattc
    10261 ttttttttat ttttttagga attatcatct ccttatattc atctgtctgt ttctcaaata
    10321 attcccatga aggcatgctg atgactctcg aagaaatatt ttcggattgt aaagcgatct
    10381 gaacttccat tgcaagcgat acttcagaac ctgtcgcgat caataatatc tcaggctcga
    10441 cattacaatc acttagtatg tatgcacctt tgtttaaatt ctctgcactg ccaaattctg
    10501 actgatctat tatcggaaga ttttgtctgg tcagcacaag tgctaccggt cctgacttaa
    10561 tttcaagcgc tgtcttccat gcctgtgaag tttcatttgc atccgcaggt cttatcactg
    10621 taagatttgg aattgatctt aaagaaataa gctgttctat cggctgatga gtcgtgccgt
    10681 cttcaccaag cgcaatgctg tcatgagtaa atacatagat tggtctgata cccatgatcg
    10741 ctgcaagtct gatcggtgca cgcatatatt ctgaaaaaat cagaaatgtc gcaccgtatg
    10801 cgatcatcgg aagtgtaaga gtaattccgc ttaagaccgc tcccattgca tgctcacgga
    10861 caccgaagtg cagatttctt ccgtcataat ttcccggctg aaaactttta tactttttta
    10921 agaatgtatt attggaaggt tccagatctg ccgaacctcc gatcatctga gggaaatatt
    10981 ctgcaattgc attaatcgtc ttacccgatg cagatctcgt agccatcttt ccttcatcag
    11041 ttgtaaatac aggaaaagct ttttcccatc cttcaatgaa ttctcccttc attaatctgc
    11101 cgatcccttt agattcatcg ggaaattttc cggaatattc tgaatacaat ttttcccatt
    11161 catctttttt cttctttcca ttttcacctt tgtctctgaa aaacttcaat acttcatccg
    11221 gaacataaaa acttttatcc ggatcgaatc caagattttc ttttgtaagt ctaagttcat
    11281 cctcaccaag cggcgaaccg tgtgatcctg ctgagtcagc tttattcgga cttccgaatc
    11341 caatgtgagt tctgattttt attaatgaag gtttatcttt ttcctgttta gcttttttaa
    11401 cagcttcaga tattttatca ggatcgttac catcactaac ttctatcacc tgccagccga
    11461 atgcttcaaa acgttttgct gtatcttctg taaatgcaat ttcagtattt ccttcgatcg
    11521 aaatgtgatt gtcatcataa agatagatca tgtttccgag tttcagatga cctgcaagtg
    11581 aagcagcttc agatgagatt ccttccatca gatcaccgtc gcttacgatt gcataaatat
    11641 tataatcaaa aatattaaat ccgggtctgt catatttggc agaaagatat ttctgtgcaa
    11701 ttgcaaagcc gactccgttt gcaaatccct gtccgagcgg acctgtagtt gtttcgacag
    11761 ctttagtcag atgtgattca ggatgaccgg gagttttact gtcgagctga cggaaatttt
    11821 ttatatcgtc aagagttagt tcatatccgg taagataaag catactgtaa agcagcatac
    11881 ttccgtgtcc cgcagacaaa ataaatctgt ccctgccggg ccagtccgga tcggatggat
    11941 cataattcat ttcatttgtc cataaagaaa atgccatcgc agcagcaccc atcggcatgc
    12001 ccggatgacc ggaattagct ttctgaaccg catctgcaga aagcagccgg atggtgttaa
    12061 tacataactc gtcgattttt gtcatgcgtt aaaatactta ttatataaat aaattttaag
    12121 attatttttg aggaaaacgg aatattgtat attgattaga aattacgaat tgaaaattta
    12181 gaaaagcgga ttatgaatta aaaaattcta ttgttaattg tcataacaga ctgattggaa
    12241 agaaagcgaa aatattctac agagacaaat gtagttattt caacaaaatc atccgcttcg
    12301 tctcctcaaa ttcacccgct accagtctat aaaaataaac tccgctcggc aaaccatcgc
    12361 cgttgaaatc aactgcatag cttccggcgt tttgcttttc attaactaaa gtcatgatct
    12421 cttttccaag attattgtaa acaataagct tcacatcaga tgtctgactt ctgacatctg
    12481 acggaattga ataacggatc aaggtcgatg gattgaaagg gttgggatag ttttgagaaa
    12541 gagaaaaaat ttccggcagt gagatattag tattactata aatgccggtt aacccgcctg
    12601 taattgtttt tagaattctt ccgttagcac aaagaatgta tcctgtaaga gaattcagaa
    12661 aaaacggctg agtaaaattt gaattataac taaactgcaa atcaaaatta tttccgccat
    12721 ctgttgtttt aaaaatattg ttttgtttga aaacccatcc tgtatcggct gtagtaaaga
    12781 agtgatgcct gatatttttt gtaaaagttg tatcaatgaa agaccagtca ttaccgccat
    12841 taactgtttt cattaaacca agcatagatg ccaaatagcc tatagaatca ttcaaaaatc
    12901 ttattttgta gaatctgtta tgagaaggat ctgcattcca taaaactgcc gaatcattat
    12961 atataatatt ccagcttgaa cctgaattgg atgtatagcc aagtctgtta acgtcagtgc
    13021 ctgcatcatc gccgaaagaa gctgaaatcc agattttatc atttgatttg caaacagata
    13081 aaactcttga atttctgaaa tattcaaaga aaggtaatga attaaagttt actgcattga
    13141 cctgtgaata tgatatatct ccggcggttt caaaaccata ccacccttca aatgaaagaa
    13201 aaaattcatt tccgtcatta taaaaatcag aaataaaatt tccaaattgt tttagcggaa
    13261 accagcttgc tccctgatca gtggtcatat aaacaatgaa ccaataacct gcttcagaac
    13321 cttcaggcac tgttgtaagt aatcctcttt gatcatcaat aaaataaata ttctgaaaat
    13381 cgagattact tggattgcta agaggaaacc agtttgccgc accgtcagta gttttaaata
    13441 atgctccgtt gtttccgcac acaaagccgg tattcgaatc aatgaagtaa atacttctga
    13501 gtctgttgga agtaatttcc ggatattcaa cccaaccttg aattgaatag gaaagtcctg
    13561 actgtaaaat aaaaactgtg attataataa attttttcat aattttaaaa tttattttaa
    13621 taagatcatt ctcttcgttt ctgtaaactc acccgcctca agtttataaa aataaacccc
    13681 gctcggcaaa ccatctccgt tgaaatcaac tgtatagctt ccggcgtttt gcttttcatt
    13741 aactaaagaa gcaactaatt ttccaatcac atcataaact ttaagtgaaa cgtgttttgt
    13801 aaccggtaat tggtaattaa tagtagtatt tggattgaat ggattcggat aattttgccg
    13861 gagaaaaaat tcctgaatta ttatattatt cagtttaatg tcacttacta tttctctgct
    13921 cccggctgta ctcaaaatca taccaccatc tcccactacc cagcaatttg tatcgattgc
    13981 agatattgat ctgatctgaa gtgctgaaat attttgtcta atccaatcaa ctccgccatt
    14041 agatgtttta aacaacaatc cggaaacact tcccaaataa cctgtatttg aattcaccat
    14101 tttaaatgaa aaaaacccgt ttccatttat tggatttgat ttcaattgcc aggacaaacc
    14161 atgatcaata cttttataaa ctttattaaa actgcaggca tatagatttc caagatcatc
    14221 attacaagat acactaaatg aataattatt tgtataagat tgccagttaa gacctccatc
    14281 acttgttatt gaagtgatat tatttccaat caatgttcca ttttgttcat tagaaaaata
    14341 aacttgaaaa attctatacc aagtttcatc caagactctt tgagaaatcc aattttcacc
    14401 accgtttgta gtcttttcaa cataaattcc gttagatttt actgatatcc atccggtact
    14461 tggattcata aaaaacattt caccaacctc accggaggga ttcaaattct gaacattcca
    14521 actattacca ccgtccgttg ttttattaat attatttgct ttgggaaacc agcccgttaa
    14581 agaatttaca aaatatatat tttctaacat ctgtgagctg tcataatata taaaattcca
    14641 atgttcacct ttgttagacg tttttaagat tccaccgcgg gtaga
//
LOCUS       DAORVQ010000078        14483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1883_length_14483_cov_5.935265, whole genome shotgun sequence.
ACCESSION   DAORVQ010000078 DAORVQ010000000
VERSION     DAORVQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1883_length_14483_cov_5.935265"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLX80_07855"
     CDS             complement(<1..444)
                     /locus_tag="PLX80_07855"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HQY20737.1"
                     /translation="MKVIRDISEFVYDKKTAVTVGTFDGVHLGHRKIIDKLNAVKNSK
                     EQRSVVITFDPHPQIVLRNRDRDIKILSTLDEKLEIFGKLDIDITYVINFTKEFSETK
                     AEDFYVNYLLKKIGMSDLILGYDHMFGKNREGNFDTLKELSAEFDF"
     gene            complement(520..1005)
                     /locus_tag="PLX80_07860"
     CDS             complement(520..1005)
                     /locus_tag="PLX80_07860"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQY20738.1"
                     /translation="MSIKTGDQMPDFSLNSFSHSDELKKYTISDYKGKKNVLLVFFPM
                     AFTGVCTEQNCTISDTVGDFASKDVEVFGVSVDGTFVQKAFAKSNNIKYPLLSDYNRE
                     VIRKLGLVHEVFAHDQKETAKRAVVLIDKEGVVKYMEITENPGVQVKFDLLKEAVENL
                     G"
     gene            1264..3378
                     /locus_tag="PLX80_07865"
     CDS             1264..3378
                     /locus_tag="PLX80_07865"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20739.1"
                     /translation="MNQGKLKYLSVFINLFLLLLSLPTINISAQSLWTLQSPLPTQNN
                     LTSVDFINENTGFACGENVMLKTTNGGINWLKTNPAPDVYITSVFFIDQSTGWITGDS
                     GNILKTIDEGKNWIPQISGTNKTLYSIHFISNDTGWAAGDSGKILRTNNGGQNWFRQE
                     TPTANTLRDIQFISDQFGWAAAGNIALRTTNGGEIWTAYEEFFPGDYISIFFISPEKG
                     WAAGATSSYLSSAVYITSDSGKSWSTQYNSNSTGRLSSIQFTSNDTGYIAGGSPFSQT
                     ILKTTNGGLNWVLQSEAGNNPLNSINMISSMEGYAVGSRGLIYKTTNGGDEWINLRSG
                     SINDFYSVWFANENTGWALGYDIYKTTDGGENWFAQLLNYPYRIESSWFISESTGYAV
                     SSNRIYKTTDGGDLWDFQTISETGLQSISFASPELGWTVGSEGLIYKTTDSGNNWVQQ
                     FYGFTHLDEVYFINENTGWISGWYGRFVKTTNGGVTWTNQFTGSNGSFSTIFFISPDT
                     GWATDGGGNIIHTTNGGDNWVYQTSGTTNSLTSIFFTSQNDGWASNNNYLIKTSDGGA
                     NWVRQTDLPLNGYFSIFFISDKIGWGVGFPGVIAKYDARLVSGNEHIIFIPENFILSQ
                     NYPNPFNPKTIINYELGFSNEVLLKVYDVLGNEILTLVNEKKTAGPYSVEFDGSDLPS
                     GIYYYRLTMNGISVSKKMMLIK"
     gene            3392..5452
                     /locus_tag="PLX80_07870"
     CDS             3392..5452
                     /locus_tag="PLX80_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY20740.1"
                     /translation="MKKIILILFTILLLTQVTKSQTNWTYQNTNTTLHYLSSVIFINE
                     NNGWTVGSNNILKTTNGGLNWVLQSCQTLNSLFSIYFISDNTGWAAGDSGKIFKTTDS
                     GILWQLQNTDIYVRLWSIFFIDPFNGWAAGDSSKIIKTTDGGVSWTQTNLSLSYNLNA
                     IFFTSGTVGYTAGDRGTIAKTTNAGENWILLPTNNTRNIYSLSFISEDTGWVCSGSGY
                     VMSTKNGGATWASQDLGVNYSLYSIKFKNSNTGWTTGNEGKVIRTTNGGSSWTTYILG
                     TNSNQTFRGLDFDSEHDHVWVVGSDGIIYRSDNGGTDWDLLTENYTKKDFNAVSFIDE
                     NTGWAAGQLIFKTTNAGNNWFQQNDFSPNGTNSIWFTSASTGYTAGSLIYKTTNGGEN
                     WYKKDSSTSYAFESISFGSPDFGIAVGYGGLISRTTNGGENWTHQSGFTTHFETVFFS
                     SPTDVYVSGGLLNLKSTNAGAVWFPIYTSSSAKHIFFPSQNTGYMIGSSTIINTTNAG
                     QNWFFQNTGSENNFSSVNFISDMTGWITGSYAFIKTTDGGKVWISQSDVPANFKSIYY
                     VSENIGWGVGYGGMIAKYNRVMVPVFTEEIILPKEFFLYQNYPNPFNPSTKISYGIPN
                     ESFVSIKIYDILGKQVMSLVNENKRPGYYDVNFNGSNFASGMYFYKIEAGSFVQTRRM
                     ILVK"
     gene            5907..6953
                     /locus_tag="PLX80_07875"
     CDS             5907..6953
                     /locus_tag="PLX80_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HQY20741.1"
                     /translation="MKDLIRIFVPLIAIIIFVDFYYETPEGVYSIGGYPVWMKDVSGK
                     QTNQTSGLSFVGGDSTNKTFISCDDIGDINLIRANEKNNPPTLEIIEIKFSDEIQILF
                     NKFKKVDMEDIYYDKHNNKIYLSIEGHEYSSYDPEVYKKKEGIYEITFNNDIMKFDSI
                     LTIKRLSLPEEVYKYTLDNIGFEGFALTENYLYLGLENLQDTSNNFTDSTYIYIVDRK
                     TNELKKSVTTKEHSISSISGLYAVDDHTLLGVDRNTRRMFYLKFDKDFNIEDSRLEVI
                     NLSIPGHEDIDKILGIATESLTLDGEGNIYVATDPWMDFYKPDLADRKKLSKDELDNF
                     YNFVPVMYKFKNIF"
     gene            6956..7372
                     /locus_tag="PLX80_07880"
     CDS             6956..7372
                     /locus_tag="PLX80_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQY20742.1"
                     /translation="MRHYRKELWFDTNSRRELINITPEVNECIAESGIMEGLLLCNAM
                     HITASVFINDDESGLHQDFETWLEGLAPEKPHSRYKHNSYEDNADAHLKRTVMGREVV
                     VAVTKGKLDFGPWEQIFYGEFDGKRKKRVLLKIIGE"
     gene            complement(7453..8214)
                     /locus_tag="PLX80_07885"
     CDS             complement(7453..8214)
                     /locus_tag="PLX80_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20743.1"
                     /translation="MKNLRLIFKSNLKGIIVLFLAFSTLTLYSCGKDGDKSDSVSDKK
                     SEETSEDSKDDDGKSSGDLEMSDDFVLTYQLQGALNGEMTMYRSGKKLKQEMNTDIMG
                     MKSISQVYIIDNFVFTVTEVNGKKMGVKTNIKDYNKKKQTAETITDPKEFENYLADKK
                     VTGSENILGKNCDIYDMGNGTEMSIHNKMYVMRIKSPEFMAVATKFDNSPSFSDDIFE
                     IPEDVNFRSINTGEKNTPGMDQLDSIMKEVEKYKK"
     gene            complement(8296..8631)
                     /gene="trxA"
                     /locus_tag="PLX80_07890"
     CDS             complement(8296..8631)
                     /gene="trxA"
                     /locus_tag="PLX80_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQY20744.1"
                     /translation="MSLIEFTDDNFDDEVLKSEIPVMVDFWAVWCGPCKIIAPLVEEL
                     ATEFTGKFKIGKLDVDNNQNVSMKYGIRSIPTLLIFKNGEMVDQLVGALPKAKIAEKL
                     NEHIKEAVN"
     gene            complement(8665..12237)
                     /gene="dnaE"
                     /locus_tag="PLX80_07895"
     CDS             complement(8665..12237)
                     /gene="dnaE"
                     /locus_tag="PLX80_07895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQY20745.1"
                     /translation="MPEFIHLHNHSHYSLLDAISSIDGLVDAAVRNKMSAVALTDHGV
                     MYGVMDFYKKAKAKGVKPVIGCEVYVAQSGTRFEKGKKNIKSIAEIEVQDLEGSDGLT
                     SANINYAHLILLAKNDIGYRNLIKLVSIGHTEGYYYKPRIDLDVLAQYSEGLVATSAC
                     AGGVISCYITREDLKTAEVMTGKYKDIFGEDFYLEIQDHGIPAEHKVIKAMPGLAKKF
                     GIKLIATNDVHYIEKEHAIAHNIYLNLSAKQSAKSFRNNGQNEVLTNLRYGTDQIYFK
                     STNEMCGLFKDFPEAIRSTLEVTEKCNLDLDTSKNYMPNFKIPEEEKSARTFDDYLEK
                     ISIDGLKKIYPDGNEIAEKRLRFELDIIKKMDFSGYFLIVQDFISYAKSIGILVGPGR
                     GSAAGSLVCYSIGITNVNPLDYDLLFERFLNPERVSMPDIDIDFQDDRREEVIQYAKD
                     KYGSDSVAQIVTFNKLAPRGVLKDVGRVLNFPFQEINELTKSIPIIFGKVTNLTECLE
                     KEIDFKNYFKTGDAAQKEDKKNLFDYCLVLENLNKNSSIHASGLVIAPGSVTDYVPLS
                     KVTGEDNVFCTQYDMNQLEDAGLIKIDFLGLKELKVIGRILTQVNKKYNKNITVDDIP
                     LDDPATYDLFSAGSTIGIFQFSKSKMREYLAKLKPKNINDLAAMNALYRPGPMDLIPE
                     FIDKRNGKMPITYLHPLMEITLKETYGVIVYQEQVMQIARDIAGFSLAEADNMRKAMG
                     KKKKEILMKIKDKFIKGASQNDVDKKIANEIFALILKFADYGFNKSHGVAYSILAFYT
                     AYLKTHYPAEFFSVSMACRKDDDTELQQLANECKKMKIRLKAPDVNYSEMDFAIKYSE
                     NSTAGAEILYGLSAIKNVGAKAAQNIIDERNANGRYLSYLDLLKRVDLRLVNKKALEN
                     LIFSGAFDSIEPIRSKLFLNMERATMYAQRYSTSEAANGQEGLFGESSSVPAIEDIII
                     EDYEEFPETEKYNKEKYAIGFYITGHPLSKYEKDIENFIELTFGDDPNEIDFKKVKNV
                     TMCGVINDFQEKQSKRGNKFGVFNLVDFYGTGECVAFSQLFEKKGHLLKNDMQVVVKG
                     IPEENGDKIKLIVEEIYSIDTFIEIFTKNLVISLDEKKTDLNNIVTIKDMVINNNGNS
                     KLFFKVTDDSSFRAFVSKEYKIKATKELISNLKLLVGENNLKLN"
     assembly_gap    12454..12553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12602..14035)
                     /locus_tag="PLX80_07900"
     CDS             complement(12602..14035)
                     /locus_tag="PLX80_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20746.1"
                     /translation="MKNLKLIEILSGLDTGEIKRLSEFIRSPFFNKNEVVISLFDYIV
                     KSMKSSDETDLEKRSVWKIIFPGEKYNDLKLRSLTSELKRLIENFLTVISGDENETRK
                     KISLTEFFEQRNMVKNFNAVSKELEDHFKNEFNRNFDFYTDFVSYERSVLNQKGRNIE
                     KDLDWIYFGMSDNIDRYFISAKLELINSLLSRKYHVLGNIAPKVKFIDEMVTFVEENL
                     SSIEKNDPLIYSEYLVLKMMTSENGDKFFNKLLNFVIKNIDRFKHNELEQVYFSVINY
                     GFNKIAMGEKIYLKKIFNVYSTFEENGFYNRKKFFQDIDFISIVIIGLYLKNIEWVEN
                     FFHKYFKKILPDFREDTGNLIKGFIDYSKKNYNEAIESLSKVNYQHSYYYLKSKETLI
                     QIYYEIGEFETMEYIADSTRHYLKRKKDVLSIHYSRYNDYLKYIAILLKARLKDKNEK
                     GILINELKKNINVIGRDWLLEKVKEIR"
     gene            complement(14204..14326)
                     /locus_tag="PLX80_07905"
     CDS             complement(14204..14326)
                     /locus_tag="PLX80_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20747.1"
                     /translation="MNSYRKYDSELYKAKKYFGVSSDFFEGKRDGNEGGGGGMF"
BASE COUNT         4606 a         2525 c         2415 g         4837 t
ORIGIN      
        1 aaaatcaaat tcggcagaca gttctttaag tgtatcaaaa tttccttccc tgttcttacc
       61 aaacatgtga tcataaccaa gtataagatc actcattcca attttcttta aaagatagtt
      121 cacataaaaa tcttcagctt tagtttcaga aaattctttt gtgaaattga taacataagt
      181 tatatcaata tctaattttc cgaatatctc cagcttttca tctagagtcg aaagtatctt
      241 aatatccctg tcacgatttc tgagtacgat ctgaggatgc gggtcaaatg taataactac
      301 gctgcgttgt tcctttgaat tctttacagc attaagtttg tcgattattt ttctatgacc
      361 gagatgaacc ccgtcaaaag taccgactgt tacggcagtt tttttatcat aaacaaactc
      421 acttatatcc ctgattactt tcactttatt agctatttaa tattaaacag aataaggcag
      481 gaatagtaag cagatattat gatacaatcc aataattctt taacctaagt tttcaacagc
      541 ttctttgagc aagtcaaatt ttacctgtac tccaggattt tcagtaatct ccatatattt
      601 cacaactcct tctttatcaa taagtacaac tgctcttttt gcagtttcct tttggtcatg
      661 agcaaatact tcatgcacaa gacctaattt tctgataact tctctgttat aatctgaaag
      721 taaagggtat tttatattat tggactttgc aaatgctttt tgtacaaaag taccgtccac
      781 gctgactcca aatacttcta catcttttga agcaaaatca cctacagtat ctgagatagt
      841 gcaattctgt tcagtacaga ctccagtaaa agccattggg aaaaatacga gcaatacgtt
      901 tttctttcct ttgtagtcac taatggtata ttttttaagt tcatctgaat gtgaaaatga
      961 attcaaagaa aagtcgggca tctgatcacc ggttttaata gacatatata agtttaaata
     1021 gttttttaga atcaaaaaga taatcatttg agattttatt aacaatccga taaaattttc
     1081 ccggaggcga atgaatttca aattacagat ttcaaatatt tgcattatga attcaatatt
     1141 taaattaaaa gatttcaatt ttcaataatt ttctaaatta taatataatt ccaacctctg
     1201 aagtctaaac tttttagttg tttgaattta tttattttat ttatatattt taaaaaaact
     1261 aatatgaatc aggggaaatt aaaatattta tcagtattca ttaatctttt tttactcctt
     1321 ctttcacttc ctactataaa tatctcagct caatcacttt ggactttgca gtcgccatta
     1381 ccgacacaaa ataatttaac atcagttgat tttattaatg aaaataccgg ttttgcatgc
     1441 ggtgaaaatg taatgttaaa gactacaaac ggaggcatca actggcttaa aacgaatcct
     1501 gcacctgatg tttatattac ttcagtcttt ttcatagatc agtcaaccgg ctggattaca
     1561 ggtgattcgg gaaatattct gaaaaccata gatgaaggta aaaactggat tccgcaaatc
     1621 agcggaacta acaagacttt atattctata cattttattt caaatgatac gggctgggca
     1681 gcaggagatt ccggaaaaat tttaagaaca aataacggag gtcagaattg gttcagacag
     1741 gaaaccccga cagctaatac actacgtgat atacagttca tatcagatca attcggctgg
     1801 gcagccgcag gaaatattgc tttaaggaca acaaacggcg gtgagatctg gactgcatac
     1861 gaagaattct ttcccggcga ttatatttca atttttttta tatcacctga aaaaggatgg
     1921 gcagccggcg caacttcttc ttatttatca agcgctgttt atattacatc tgacagcgga
     1981 aaatcctggt caacgcaata taatagtaac tcaaccggaa gattatcttc tattcaattc
     2041 acatcaaatg acacaggata tattgccgga ggcagtcctt tttctcagac aattttaaaa
     2101 acaactaacg gtggactaaa ctgggtatta caatctgaag cgggtaataa tcctttgaat
     2161 tcaataaata tgatctcgtc tatggaaggt tatgctgtcg gcagcagagg attaatttat
     2221 aagacaacaa acggaggaga cgaatggata aatcttagat caggttcaat aaatgatttt
     2281 tattcagttt ggtttgcaaa tgaaaacacc ggctgggcat tgggatatga tatttacaaa
     2341 accactgacg gaggagaaaa ctggtttgca cagttattaa attatccata cagaattgaa
     2401 tcctcctggt ttatttcaga atctacaggc tatgcggtat ccagcaacag aatatataaa
     2461 accactgacg gaggagatct ttgggatttt cagacaatat ccgaaacagg acttcagtca
     2521 atttcttttg cttcacctga actcggctgg acagtcggat cagaaggatt gatatataaa
     2581 actactgaca gcggcaataa ctgggtacag cagttttacg gcttcactca tttggatgaa
     2641 gtctatttta taaatgaaaa caccggctgg atttcaggct ggtatgggag atttgtaaaa
     2701 accaccaacg gcggtgtcac ttggaccaat caattcacag gttcaaacgg cagttttagt
     2761 acaatattct ttatctcacc ggatacagga tgggcgacag acggaggcgg aaatattata
     2821 catacgacaa acggcggcga taactgggtt tatcagacaa gcggtacaac gaattcttta
     2881 acttcaattt tttttacatc ccaaaatgac ggctgggctt caaataataa ttatttaatc
     2941 aaaacttctg acggcggagc aaactgggtc agacaaactg acctaccttt aaacggttac
     3001 ttttcaatct tttttatttc agataaaatc ggatggggtg taggttttcc gggagtgatt
     3061 gcaaaatatg atgcaagatt ggtttcggga aatgaacata taatttttat tccggaaaat
     3121 tttatactgt cacagaatta tccaaatcca tttaatccaa aaacaattat aaattatgaa
     3181 ttaggatttt cgaatgaagt cttattaaaa gtgtatgatg ttttgggaaa tgaaatatta
     3241 actttagtaa atgagaaaaa aacagcgggt ccttattcag tggaatttga cggaagtgat
     3301 ctgccgagcg gaatttatta ttatagatta acgatgaatg gaatttcggt ttcaaaaaaa
     3361 atgatgctga taaaataact ttaccgtaac tatgaaaaaa ataattttaa ttttgtttac
     3421 aattttactt ttgacacaag taacaaaatc tcagacgaac tggacttatc agaataccaa
     3481 tactacgctt cattatttat cttccgttat ttttataaat gaaaataacg gatggactgt
     3541 cggctccaac aatattctga aaacaacaaa cggcggatta aactgggtct tgcaatcctg
     3601 tcagactttg aattcacttt tttcaatata ttttatttct gataacaccg gctgggctgc
     3661 aggtgattca ggaaaaattt ttaaaactac agattcagga atattatggc agttacagaa
     3721 taccgatata tatgtacggc tttggtccat tttttttatt gacccgttta acggctgggc
     3781 agcgggcgat tcatcaaaga taattaaaac tactgacgga ggagtaagct ggactcagac
     3841 taatttatct ttatcttata atcttaatgc aatcttcttt acttccggta ctgtcggtta
     3901 cactgccggc gacaggggaa ccattgcaaa gacaaccaac gcaggtgaaa actggatatt
     3961 actgccaaca aataatacaa gaaatattta ttcattatct tttatttctg aagataccgg
     4021 ttgggtatgt tcgggaagcg ggtatgtaat gagtacaaag aacggcggag cgacatgggc
     4081 atcacaggat ctcggtgtta actatagttt atattcaata aaatttaaaa acagcaatac
     4141 cggatggaca accggcaatg aagggaaagt aataaggact acaaacggag gatcaagctg
     4201 gactacttat attcttggaa ccaattctaa tcagacattc cgcggacttg attttgacag
     4261 tgagcatgat catgtatggg ttgtcggatc agacggaata atttacagat ccgacaatgg
     4321 cggaacagac tgggatctgc tgacagagaa ttatacaaaa aaagatttca atgcagtttc
     4381 gtttattgat gaaaataccg gatgggcggc aggtcagtta atttttaaaa cgaccaatgc
     4441 cggtaataat tggtttcagc aaaatgactt ttcaccaaat ggtactaact caatttggtt
     4501 tacttcagcg tcaacgggtt atacagccgg aagtctaata tataaaacta caaacggcgg
     4561 tgaaaactgg tacaaaaaag attcttctac ttcttatgca tttgaatcaa tttcattcgg
     4621 ttcacctgat tttggaattg cagtcggata tggaggtctg atctcaagga caaccaacgg
     4681 cggtgagaac tggactcatc agagcggatt tacaactcat tttgaaactg tcttcttttc
     4741 ttccccgaca gatgtctatg tttcaggtgg cttattgaat ttgaaatcta caaatgccgg
     4801 agctgtctgg tttccaattt atacttcctc cagcgcgaaa catatatttt tcccatcgca
     4861 gaatacagga tacatgatag gcagcagtac tattatcaat actactaatg caggacagaa
     4921 ctggtttttt caaaacaccg gcagcgagaa taatttttca tcggttaatt tcatttctga
     4981 tatgaccggc tggataaccg gaagttatgc gtttattaaa actacagacg gcggtaaagt
     5041 ctggataagt cagtctgatg tgcctgcaaa ttttaaatct atctattacg tatcagaaaa
     5101 tatcggatgg ggtgtcggtt atggcggaat gattgcaaaa tataacagag tcatggttcc
     5161 ggtatttact gaagaaatta tattaccgaa agaatttttt ctctatcaga attatccgaa
     5221 ccctttcaat ccatctacaa agatcagcta cggcattccg aacgagtcat ttgtatcaat
     5281 aaagatttat gacatactcg gtaagcaggt aatgagtctt gtaaatgaga ataaacgacc
     5341 cggatattat gacgttaatt tcaacggatc taactttgca agcggaatgt acttctataa
     5401 aatagaggca ggaagttttg ttcagacaag gcgtatgata ctcgtgaaat aatttcaatt
     5461 taaaaaatac aaattacaaa ttacgaatac gtaatattaa atattattga aattgattaa
     5521 aattttttat ctttcccgaa atgccatttt cgggaaagat attttttatg ttttattatg
     5581 gaaaattttt taaaactgac ttaaagtttt tttatatatt ttgagaaact attataggaa
     5641 aacatttata tgaatactga gactctaatt ctaaagagta tttcaataac atttacagtt
     5701 ataaatatat aaattaactt taccacttat aatatttagt aatttataat cacatgaaac
     5761 catttacttg tcttaatgaa aactcatttt aatcaactga ttatattaat ttcaaatctg
     5821 tatcatttcc aaaaatttaa ttgaactctc tttaataaaa ggagttacat taatctttat
     5881 aaaaaaattt ctattaaaat tatttaatga aagacctgat aagaattttc gtaccgctta
     5941 tagcaataat tatttttgtt gatttttatt atgagacacc tgaaggagtt tattctatcg
     6001 gcggttatcc ggtctggatg aaagatgtat caggtaaaca gaccaatcag acatcaggac
     6061 tttctttcgt cggaggtgac agtacaaata aaacatttat atcatgtgat gacattggtg
     6121 atattaattt gatccgtgca aatgaaaaaa ataacccgcc tactctggaa atcattgaga
     6181 taaaattttc tgatgagata cagatccttt ttaataaatt taaaaaggtc gatatggaag
     6241 atatttatta tgacaaacat aataacaaaa tttatttatc tattgaagga catgaataca
     6301 gttcttatga ccctgaggtc tacaaaaaaa aggagggtat atatgagatc acatttaata
     6361 atgacattat gaaatttgat tccatcctga caattaaacg attaagtctt cctgaagaag
     6421 tatacaaata tacacttgat aacattggtt ttgaaggatt tgctctaact gagaattatt
     6481 tatatttagg actggaaaat ctgcaggata catcgaataa tttcacagac agtacttata
     6541 tttatatagt ggacagaaaa acaaatgaac ttaaaaaatc agtcacgact aaagagcata
     6601 gtataagttc aatttccgga ctttatgctg tagatgatca tacactttta ggagtggata
     6661 gaaatacccg aagaatgttt tatctgaaat ttgataaaga tttcaacatt gaagattcaa
     6721 gattagaagt aattaatctt tcaattccgg gacatgaaga cattgataaa attcttggca
     6781 tagcaaccga atcattgaca ttagatggtg aaggaaacat atatgttgct accgacccct
     6841 ggatggattt ttataagcct gatctggctg acaggaagaa actttcaaaa gacgagctgg
     6901 ataattttta taattttgtt ccggtcatgt ataaatttaa aaatattttt tgaaaatgag
     6961 acactacaga aaagagcttt ggtttgatac aaattcacgg agagaactta taaatattac
     7021 tcccgaagta aatgaatgta ttgctgaaag cgggatcatg gaaggacttt tgctttgtaa
     7081 tgcaatgcac attactgcaa gtgtatttat aaatgatgac gagtcgggac tgcatcagga
     7141 tttcgaaaca tggctggaag gactcgctcc ggaaaaaccg cattcgagat ataagcataa
     7201 ttcatatgaa gacaatgcag atgcgcattt gaaaagaact gtaatgggca gagaagttgt
     7261 cgttgcagtt acaaaaggta aattagattt cggtccgtgg gaacagatct tctatggaga
     7321 gtttgacggt aaaagaaaaa aaagagtttt gttaaagata attggagagt gattttaaaa
     7381 gcgacaatta aatattaata ttcagacttt gataaatttt ataaaagttg tcaaagtctg
     7441 aacatatatt ttctacttct tatatttttc tacttccttc ataatagaat cgagctgatc
     7501 cattccgggt gtattttttt cgccggtatt aatacttctg aaatttacat cttcaggaat
     7561 ttcgaatata tcatctgaga atgaaggact gttatcaaac ttagtcgcaa cagccataaa
     7621 ctcaggtgac ttaattctca tgacatacat tttattatga atagacatct cagttccgtt
     7681 acccatatca tagatatcac agttctttcc aagaatattt tctgaacctg taactttttt
     7741 atcagcaaga taattctcaa attccttagg atcagtaata gtctcagcag tctgtttctt
     7801 tttgttgtaa tctttgatat ttgttttcac acccattttt tttccgttta cttcggtaac
     7861 agtaaaaaca aaattatcga ttatataaac ctgacttata cttttcattc ccatgatatc
     7921 agtattcatt tcctgtttca atttttttcc gcttctgtac atagtcattt caccgttcaa
     7981 agcaccttga agctgatagg ttaaaacaaa atcatctgac atttccaggt cacctgatga
     8041 ttttccatca tcatcctttg aatcttcaga agtttcttca gattttttat ctgagacaga
     8101 atcagattta tctccgtctt ttccacaaga atacaaagtc agtgtagaaa aagcgagaaa
     8161 taaaactata atacctttca aattgctttt gaaaattaag cgtaagtttt tcattgttat
     8221 ataaattaaa ttaataaaaa cgggatcata ctttttagta agatcccgga ttatatgatt
     8281 caaatatttt gaattttagt ttacagcttc ttttatatgc tcatttaatt tttctgcaat
     8341 cttagctttt ggtaaagcgc cgacaagctg atccaccatt tctccatttt taaagatcag
     8401 taaagtagga atgctgcgaa ttccgtattt catggaaacg ttttgattgt tgtctacatc
     8461 cagttttccg atcttaaact ttccagtgaa ttctgttgca agctcttcta caagaggagc
     8521 gataatctta catggtccgc accagacagc ccagaaatca accattacag ggatttcaga
     8581 ttttaaaact tcatcatcaa aattgtcgtc tgtgaattca attagactca ttttattttc
     8641 ctttctgtta atttattaaa atatttaatt tagctttaag ttattttctc caactaataa
     8701 tttcaagttt gaaattaatt cttttgttgc ttttatttta tattcttttg agacaaatgc
     8761 tctaaagctt gaatcgtcag taactttaaa aaacagtttc gaattcccgt tgttatttat
     8821 caccatgtct tttatggtta caatattatt caaatctgtc ttcttttcat caagactgat
     8881 cacaagattt tttgtgaata tttctataaa tgtatctata ctgtagatct cctctactat
     8941 taattttatc ttatcaccgt tttcctctgg aatgcctttg acaacgacct gcatgtcatt
     9001 cttaagcaga tgacctttct tttcaaacaa ttgtgaaaaa gctacacact cgcctgttcc
     9061 ataaaaatca acaagattaa agactccgaa cttgtttcct ctttttgact gcttttcctg
     9121 aaagtcatta atgactccgc acatggttac attttttact tttttaaaat ctatttcatt
     9181 gggatcatcg ccgaaagtca gttcaataaa attctctatg tccttttcat attttgaaag
     9241 cggatgcccg gtaatataaa atcctattgc atatttttcc ttattgtatt tttccgtttc
     9301 aggaaattct tcataatcct caatgataat atcttctata gcgggtacag atgaagattc
     9361 cccaaacagt ccttcctgtc cgttagcagc ctcactcgta ctgtatcttt gcgcatacat
     9421 tgtagctctt tccatattta aaaataactt actgcgtata ggttctatgg agtcaaacgc
     9481 acctgagaag atcaggtttt caagagcttt tttattgacc agccgcagat ctactctttt
     9541 cagcaggtcg agataactga gatatcttcc gtttgcattt ctctcatcaa taatattctg
     9601 cgcagctttt gcaccgacat tctttattgc actcagtccg tagagaatct ctgcacctgc
     9661 agtactgttt tcagaatatt ttattgcaaa atccatttca ctataattca catcaggagc
     9721 tttaagtctg atcttcattt ttttacattc gttcgcaagc tgctgaagtt cagtatcatc
     9781 atcttttctg caagccatcg agactgaaaa aaattctgcg ggataatgtg tctttagata
     9841 tgctgtataa aaagcgagta tagaatatgc aacaccatgg gatttattaa aaccatagtc
     9901 tgcgaatttc aggatcaaag caaagatctc attggcaatt tttttatcga catcattctg
     9961 tgaagcacct ttgataaatt tatctttgat cttcatcaga atttcttttt ttttcttacc
    10021 cattgccttc ctcatattat ctgcttcagc aagagagaat cctgcaatgt cacgtgcgat
    10081 ctgcatcacc tgctcctgat acactatgac tccgtaagtt tctttcaaag taatttccat
    10141 caaaggatgc aaatatgtaa tcggcatttt accgtttctc ttatctatga attcaggtat
    10201 gagatccatc ggacccggac ggtacaacgc attcattgca gcaaggtcat tgatattttt
    10261 cggtttcagc tttgcaagat attcgcgcat cttacttttt gaaaactgga aaattccgat
    10321 ggtcgagccg gctgaaaaga gatcatatgt agccggatcg tcaagaggaa tatcatcaac
    10381 agtaatattc ttattatatt ttttatttac ctgtgttaaa attctcccga taaccttcag
    10441 ttctttcaga ccaagaaagt caatcttgat aagacctgca tcttcaagct gattcatgtc
    10501 atattgtgta cagaagacat tatcttcacc ggtaactttt gacaaaggca cataatctgt
    10561 tacgctgccc ggagctatta caagacctga ggcatgaatg gaagaatttt tattcagatt
    10621 ttccagtaca agacaatagt caaacagatt tttcttatcc tctttctgag ctgcatctcc
    10681 ggtcttgaaa taatttttaa aatcaatctc tttctcgaga cattcggtta gatttgtaac
    10741 ctttccaaat attataggga tactttttgt taattcattt atctcctgaa aagggaaatt
    10801 caaaactctg ccgacatctt tgagaacacc gcgtggagcg agtttattga atgtaactat
    10861 ctgcgctaca gagtcagaac cgtatttgtc tttcgcatac tggatcactt cctcacgtct
    10921 gtcatcctga aaatcaatat caatatcggg catggaaact ctctcaggat tcaggaatct
    10981 ttcaaagaga agatcataat caagcggatt tacatttgtg atcccaatgg agtaacagac
    11041 gagacttcct gcagcgctac ctcttcccgg acctacgagg ataccaatac ttttagcata
    11101 actaataaag tcctgtacga tcaggaagta acctgagaaa tccattttct taattatatc
    11161 aagctcaaat ctcaatctct tttcagcaat ttcatttccg tcaggatata tttttttcaa
    11221 tccatcaatt gagatctttt caagataatc atcaaacgtc cttgccgatt tttcctcttc
    11281 aggtatttta aaattcggca tgtaattttt tgaagtgtcg agatcgagat tacatttctc
    11341 tgtcacttca agtgtagatc tgatcgcttc aggaaagtcc ttaaagagac cgcacatttc
    11401 atttgtagat ttgaaataga tctgatcggt gccgtatctt agatttgtca gaacttcatt
    11461 ctgaccatta ttcctaaaag attttgctga ctgttttgcg cttagattaa gataaatatt
    11521 atgagcaatg gcatgttcct tttcaatata atgaacgtcg ttagtagcga tcagcttaat
    11581 accgaatttc ttagcgagac ctggcatggc tttgatcact ttatgctcgg caggaattcc
    11641 gtgatcctga atttcgagat aaaaatcttc accaaaaata tctttgtatt tacctgtcat
    11701 gacctcagcg gttttcaagt cttctcttgt gatatagcat gagatgacac cgcctgcgca
    11761 tgctgaagtc gcgacaagtc cttcactgta ctgtgcgagc acatcaagat caattctcgg
    11821 tttataataa taaccttctg tatgtccgat ggaaacaagc ttgatcagat ttctgtaacc
    11881 gatatcattt tttgcaagga gaatcagatg agcgtaattt atattagcag aagtaagacc
    11941 gtcagaacct tcaagatcct gcacttcgat ctctgcaata gatttaatat ttttttttcc
    12001 tttttcaaat ctcgtacctg actgtgcgac atatacttcg caaccaatga caggtttgac
    12061 tccttttgct ttcgcctttt tataaaaatc cataacaccg tacatgactc cgtgatcggt
    12121 aagagcgaca gcagacattt tatttctaac tgctgcatcg acgagtccgt caatggatga
    12181 gatcgcatcg agcaatgagt aatgagagtg attatgtaag tgaatgaatt ccggcatata
    12241 atttttagta aggaagtaag gtttaatata ttgaaaataa aaaattaatg aaataagaaa
    12301 ttaatgtaat ttactttggg aatattaatt gaagggaaaa aaaatatgaa tttgagagaa
    12361 gcgaagcgat gaaggaatct cagaaattat atacctgatt ttaattggat tttatagtcg
    12421 tcaattcgga gattgcttcg catgaagagc acannnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnttcggaa aggcatttat ttgacatttg aattttgcag cttgaaattg
    12601 attatcttat ttctttcact ttttcaagaa gccagtctct gccgatgaca ttgatgtttt
    12661 ttttgagttc gtttattaga atgccttttt cgtttttgtc tttaagtctg gctttgagta
    12721 gaatggctat gtatttcaga tagtcgttgt atctgctgta gtgaatagac agtacatctt
    12781 ttttccgctt gaggtaatgc ctggtggaat cagcaatata ttccattgtc tcaaactcac
    12841 caatttcata atagatctga atgagagttt cctttgattt caaataataa taagagtgct
    12901 gataatttac tttgctcaga gattcaattg cttcgttgta attcttttta ctgtagtcaa
    12961 taaatccttt gatcagattc cctgtatctt ctctaaagtc aggaagtatt tttttgaaat
    13021 acttatgaaa gaaattttca acccattcta tatttttcag ataaagaccg atgatcacaa
    13081 tgctgataaa atctatatcc tgaaagaatt tttttcgatt gtaaaagccg ttctcttcaa
    13141 acgtagaata gacattgaat atttttttta aatagatttt ctcacccatc gcaattttat
    13201 tgaaaccgta atttatcaca gagaagtaaa cctgttcgag ttcgttatgc ttaaatctgt
    13261 caatattttt aatcacaaaa ttcaaaagtt tattaaaaaa tttatctccg ttttctgagg
    13321 tcatcatttt caggacgaga tattcagaat aaataagcgg atcatttttt tcaatggacg
    13381 aaagattttc ttcgacgaat gtaaccatct catctatgaa tttcaccttc ggggctatgt
    13441 ttccgagcac atgatatttt cggcttagta aagagtttat gagttcgagc tttgcggaaa
    13501 tgaaatacct gtctatatta tccgacattc cgaagtagat ccagtcgagg tctttctcaa
    13561 tgttcctgcc tttctgattc agcacagatc tttcgtagct gacaaagtca gtatagaaat
    13621 caaagttacg gttgaattca tttttaaaat ggtcttcgag ttcttttgaa actgcattaa
    13681 aatttttgac catatttctc tgctcgaaga attcagtcag tgagatcttt ttccgcgttt
    13741 cattttcatc acctgaaata acagtgagaa aattttcgat cagtctttta agttcagaag
    13801 taagtgagcg cagttttaga tcattatatt tttcacccgg aaaaatgatc ttccacacgg
    13861 atcttttttc aagatcggtt tcatcggatg acttcatgct tttgacaatg tagtcaaaca
    13921 gcgagatgac gacttcattt ttattaaaaa aaggtgagcg gataaattca gacagacgtt
    13981 taatttcacc cgtatcgaga ccgcttagaa tttctatcag ttttaaattc ttcatagaag
    14041 ttttgaatat tccttactgc tatattattt ctaaaaatga cttaccatgc aaacttatgc
    14101 ttttttaccc aataaatgaa tacaaacagg agacaacatt tcccacaaac ggcaaatcct
    14161 ttccgggagt tttttcggaa aaaacggtta aaaccgttta aaatcaaaac attccccctc
    14221 ccccaccttc atttccatct cttttccctt caaaaaaatc actcgaaacg ccaaaatatt
    14281 tctttgcttt gtatagctca gaatcatatt ttcgatatga attcatttta aaaaatgcag
    14341 atacataata ttaattttta cctcaaaggc tcgaaggctc gaaggagata tgacattatg
    14401 tatttaaact ttgagtcctg gaggcttaga gtctttgaga cagtaccaaa gttttcatac
    14461 aagttgaata tttggaaatg agc
//
LOCUS       DAORVQ010000079        14308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1923_length_14308_cov_6.098646, whole genome shotgun sequence.
ACCESSION   DAORVQ010000079 DAORVQ010000000
VERSION     DAORVQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1923_length_14308_cov_6.098646"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="PLX80_07910"
     CDS             complement(<1..1786)
                     /locus_tag="PLX80_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY20748.1"
                     /translation="MKKTNIYLRFILIPVFAITFLIITGSNNFSNNFKISKALNSEMS
                     SLDANQELHIWITFRDKGTDVNELLAHPERYLTERAIKRRMKVKPANALVDYTDIPLN
                     QSYISDLSRLRIKIKNRSKWFNSVSCYADISQIENLADKEFVSKIDIVRTYKKNNDNI
                     EFENNSSEVTHENNFLENDNSTLLNYGNSLEQMEIINATVPHDSGYFGQGVIIANFDT
                     GFDNLQHPAFDSLRARGFRSYDFVNHDTIVGNQNGQMGEGSHGTATLSLVGGYSPGNL
                     ISPAFRSQYILAKTENTDSETPLEEDNWIAAAEWVDSLGADIITSSLGYLTMDQGSVR
                     SYDWTWMNGDSCVITIGADLAVNKGIIVCNSAGNNGSHNSHNTLNAPADGDSVLSIGS
                     VNSNGNRSSFSSVGLTTDGRIKPEVCAMGSGNRVAASGAGNTGYSSGSGTSFSCPMTA
                     GVCAQILSANYNITPMQMRQLLTSTANNSSSPDRFTGWGTINAWEALKLGRILTGVNP
                     VNVVNSVPDGFSLSQNFPNPFNPRTIINYELGITNDVKLKIYDVLGNEVAELVNEKQN
                     AGSYSVEFDGSGFASGIYFYRLEIDGNII"
     gene            complement(2149..4188)
                     /locus_tag="PLX80_07915"
     CDS             complement(2149..4188)
                     /locus_tag="PLX80_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY20749.1"
                     /translation="MKKELKFYKLTFLTLAVIFILSLTGSNNFTNNFKISKNLSLLLN
                     DMQQNEKVLIWISFTDKGENSSSLLADPETLVTKRSLDRRKKVKPAGKLVDLTDIPVS
                     RNYISDLQNSGIEVKQKSRWFNRISCYADKDQINNIVSKDYINGIDIVMKFRKKYDDA
                     ESGPGNEFLETDGNNNQNGLQYALNYGTSETQMEMINAPMGHDSGYYGQGIIIASFDA
                     GVDNLEHPCFDSLRARGIRTFDFVNNDTIVADQTGQMGAGWHGTMTLSLAGGYKPGSL
                     ISPAFRSHYIIAKTENTDSETPLEEDNWIAAAEWADSLGADIITSSLGYLGMDNGSSH
                     SYNWTWMNGDSCFLTIAADLAVNKGILVINSAGNEGSHASHNTIMSPADGDSVIAVGS
                     VTSSSTRSSFSSVGLTIDGRIKPDIMAMGSNNKIAQTGSGNTGYSSGSGTSFSCPMTA
                     GACAMILSANPDLTPIQVREILYMSADNYNSPDRFYGYGIINTWEAIKLALPKKLNLT
                     AVIEGLYDANSNTMAADTVTANIRSSSSPYNILDQEKGVLNSNGTLTLEYGNVNNGVN
                     YYLQILHRNALETWSKNTQSFISNVLNYNFTAAAVMAFGDNMLLKGSKWVFYSGDVNS
                     DKSVDLGDLSIIDNDANDFLTGYNDSDLNGDGLTDIADLLYADNNAGNFVVASTP"
     gene            complement(4410..5180)
                     /locus_tag="PLX80_07920"
     CDS             complement(4410..5180)
                     /locus_tag="PLX80_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY20750.1"
                     /translation="MSSKILKYIFIELIRNRFIIFYAIFLFVLSSAIIYIGHDSSKAV
                     VTLLNLILFIIPLVSLIFGTIHFYNSEEFIQLLLTQPVDRKSIFLAEYLGIAFSLSLA
                     FIAGAGLPLIYYGITVPVMFLLGTGVILTFIFVSLAFFSSVINKDKVKGIGLSVIIWL
                     FMSVIFDGIIFVILFAFNDYPLEKFILIFTSLNPVDLSRILILLQINISALMGFTGAL
                     FRDFFGSAVGMITAFILLMTWIFIPGFVSMKVFNKKNF"
     gene            complement(5177..5893)
                     /locus_tag="PLX80_07925"
     CDS             complement(5177..5893)
                     /locus_tag="PLX80_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY20751.1"
                     /translation="MIRTDKLYKRYGKFEVLKSIDINLGKGQITGLMGPNGSGKSTLL
                     KSILGLVIPDSGKIYVKDSDIKNKFDYRKYIGYMPQIANYPENLKVKELFKIVKDIRT
                     DVKEYDVELFENFKLGEIENKNFGQLSGGYKQRVTAAIAFLFSPEILILDEPTSSLDP
                     VSTEILKDKILKSKSLGRLVIVCSHLVPEVSELADRLVYLIEGKILFNDKISNLINGS
                     GETLGKTISRLLQNKTDKII"
     gene            complement(5880..7088)
                     /gene="nosD"
                     /locus_tag="PLX80_07930"
     CDS             complement(5880..7088)
                     /gene="nosD"
                     /locus_tag="PLX80_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HQY20752.1"
                     /translation="MISAKEITVDPAGQINLIKEALRISEDGDRILIKSGYYKEYDLI
                     AEKRVEIIGEEGSLIDIDFKGDGIILRKDSSSVSGLTIKNMVVSYIKDIAGIKLEKSN
                     FSKVFNNRLFDTFFSIYLENSSDCLVSGNTIVGNAATETSSGNGIHLWKCKNVMIENN
                     YISGQRDGIYLEFVDFSIVRNNFSEKNLRYGLHFMYADSNSYSHNTFKNNGAGVAVMY
                     SEKIEMTHNTFIHNWGAASFGILLKEIRRSKIFGNIFIENTTGIYMEGCNDIYLFEND
                     FIKNGWALKIMGSCSDNFLERNNFIENTFDISSNSSRNSNFYDSNYWDKYGGYDINKN
                     GTGDVPYRPVSLYSMMLEKMPDAIILLRSFIVDLIDITEKAMPVFIPESLADQNPKMS
                     KYEFNIYDKN"
     gene            complement(7165..7659)
                     /locus_tag="PLX80_07935"
     CDS             complement(7165..7659)
                     /locus_tag="PLX80_07935"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HQY20753.1"
                     /translation="MKKNSLKNLYNNHLKLRSIIYIVICLTFTSCSKELSPINFGKDM
                     CDHCKMTIVDKKFGGELVNDKGKAYKFDAIECMVEYEEEKRSDSENEIFTLYTSYTID
                     ILNPGELIKSDDAIFVLSESIKSPMGGDLASYSNKTEAENFIKENGGELFDFKSLKEK
                     FIKK"
     gene            complement(7628..8200)
                     /locus_tag="PLX80_07940"
     CDS             complement(7628..8200)
                     /locus_tag="PLX80_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20754.1"
                     /translation="MKKISRILIVVFSLALIGVFFVPIWDISLQAPQYPEGLGMKIWI
                     NKMSGDLDIINGLNHYIGMKTIHPESIKELTVMPYVIGFFIAAGIFVSVLNKPKFLLV
                     WIVLFVIAGTVGLYDFYLWEYDYGHNLDPTAAIKVEGMNYQPPLIGSKNLLNFKAVSM
                     PDIGSYIFFLTGLIFIYVYINEKKFFKKSL"
     gene            complement(8251..10233)
                     /gene="nosZ"
                     /locus_tag="PLX80_07945"
     CDS             complement(8251..10233)
                     /gene="nosZ"
                     /locus_tag="PLX80_07945"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HQY20755.1"
                     /translation="MKKTNTLSKSIFKGILILIIAASFYSCSEKDSKTLSMSGDEASK
                     VYIEPGKYDEFYYFMSGGFSGQMSVYGIPSGRMLKVIPVFSVNPENGWGYTEESKPML
                     NTSYGFIPWDDAHHSELSQTDGVPDGRWVFINGNNTPRIARIDLKTFKTTEIIEIPNS
                     SGNHSSPFTTPNTDYVVAGTRFSIPVENRDVPITSYKEEFKGALSFIKVDKTTGRMNI
                     DYQIITPPYDYDLSHSGKGPSDGWFFFTCYNSEEANSLLEVNASQKDKDFILAINYKK
                     VDEIVKGGAGKEMSTKYAHNIWNDETHSATSTMINTVKTLLPEDIKGAAYYIPCPKSP
                     HGADVDPTGEYITAGGKLSTVIPVFAFSKITKAIAENKTDGEAGGIPILNYEACLSGE
                     VKDPGLGPLHTEFDGKGFAYTSSFISSEVVKWNIETKQVVDRIPVYYSIGHLSIPGGD
                     SKKPWGKYLIALNKMTKDRYLPTGPELTQSAQLIDISGDKMRILLDFPTIGEPHYAQG
                     IPAELVTKDQIRIYKIEDNKNPHAIKSILDARVERNGSVVHVYFAATRSHFTPDNIEG
                     VKLGDTVYFHMTNLEQDWDIPHGFAVMGNNNSEMLVMPGETCTLKWVPSRVGIMPMYC
                     TDFCSALHQEMQGYIRVSAQGSDVPIKFSLGDEKKE"
     gene            complement(10284..10703)
                     /locus_tag="PLX80_07950"
     CDS             complement(10284..10703)
                     /locus_tag="PLX80_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQY20756.1"
                     /translation="MNKQNSSILALIFIITMISYTGCGGNDPELGIGPVKEIKLEPAI
                     NENLVATGKQIFDMKCITCHQINSKLVGPPLKDVTKRRKPEWIMNMILNPVQMVQENM
                     IAKTLATSGQYPTPMTFQNVSQDDARAILEYFRNNDK"
     gene            complement(10700..11479)
                     /locus_tag="PLX80_07955"
     CDS             complement(10700..11479)
                     /locus_tag="PLX80_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQY20757.1"
                     /translation="MDKIINTRLTDIINYNFCAIDLFEKYNFNYCIKGNSTLGNILKE
                     NNVRPEKFINEFNRLNSFTAGYMKFSDWTVDFMCDYIIANHHRYIRKIFPLIIRSAVD
                     LVKRGIIRSDISDKLKIMSYDFQIHMQKEEKFIFPEIKKINEFDLNKSKYDIPPFGSI
                     LSPVKVLIKEHETAFENFSDIKKECDNFKTEQTGKKTGGGAGNDKKDLFYKMLRDFEI
                     DLHLHFHLENNILFLKAVKSEKKLLKNNSKNYLNRIKTSKK"
     gene            complement(11695..12393)
                     /locus_tag="PLX80_07960"
     CDS             complement(11695..12393)
                     /locus_tag="PLX80_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQY20758.1"
                     /translation="MDSSILKNINLFSELDENEMDKIYSHSSIKTYDKGEMIFFDTEP
                     YIGFYIVIEGGVKIFKISKDGREHVIHFIFPTNTFAEAPLFEHYDKIRREEFTYPANS
                     MAIENNTRLMLIRSDIFYNLLEKNKTICFKMLSGLSKKLRHLNNHIESLSQGVSVRLA
                     KYLLDELNSSDEKNKGIIELKISKHDLAAYLGTIDETLSRCLKKLQDNNVIIVKAKRI
                     QILDISTLQKTAIG"
     gene            complement(12386..12640)
                     /locus_tag="PLX80_07965"
     CDS             complement(12386..12640)
                     /locus_tag="PLX80_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HQY20759.1"
                     /translation="MDPVLITGLIAASLTTFAFLPQSLRALRTKHTADISLPMLIMLE
                     IGVVVWIIYGSMISDIPLLAANSISFIFVSATLIMKLKYG"
     gene            complement(12784..14148)
                     /locus_tag="PLX80_07970"
     CDS             complement(12784..14148)
                     /locus_tag="PLX80_07970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQY20760.1"
                     /translation="MSNKNTKSDATEKIVSLSKRRGFIFQSSEIYGGLNGCYDYGPLG
                     VELLRNIKESWWKEMTYRENIEGIDSSILMHPRVWEASGHVEHFSDPMVDCKECKGRF
                     RADQLEESDCGNKGFKGRKAVKCFEEGKFTEARNFNLMFKTHVGPLEESSNAVFLRPE
                     TAQGIYVNFNNVRESSRQKIPFGIAQIGKAFRNEINTKNFLFRTREFEQMEMQFFVNP
                     ADDAKWYDYWKDQRYNWFIKYGMDKDKIKFHEHEKLAHYAKAAVDIEFEFPFGWGEIE
                     GIHNRTDFDLKRHTEFSGKKIEYFDDQTKERFTPFVIETSAGASRSFFAFLCNAYEEE
                     ELTKENSDGTASSDTRTVLRFHPSLAPVKAGIFPLVNRDGMQDIAHNITDDLRKSFNV
                     FYDDSGAVGRRYRRQDECGTPFCITVDSDTLTDNSVTIRHRDSMEQERIQKDNLNEYL
                     KSKI"
BASE COUNT         4258 a         2816 c         2224 g         5010 t
ORIGIN      
        1 ctataatatt cccatctatt tccaatctgt aaaaatatat cccgctcgca aacccgcttc
       61 cgtcaaactc cacagaataa ctccctgcat tctgcttttc atttacaagc tctgcgactt
      121 cattcccaag cacatcataa attttcaatt tcacatcatt cgtaattcct aattcgtaat
      181 tgataattgt tcgggggttg aatgggtttg gaaaattctg tgacaaactg aaaccatccg
      241 gtacagaatt tacaacattt accggattca cacccgtcag gattctgccg agtttgagag
      301 cttcccatgc gttaatcgtt ccccatcctg tgaatctgtc cggtgacgaa ctgttatttg
      361 cagtcgaagt taataattgt ctcatctgca tcggagttat attatagttt gctgaaagta
      421 tctgtgcgca gactcccgct gtcatcggac atgagaatga agtgccgctg ccggaggaat
      481 aaccggtatt acctgcgcct gatgctgcca cccggttacc gcttcccatt gcgcagactt
      541 caggtttaat cctgccgtca gttgtcagac ccactgatga aaaagaagat ctgtttccgt
      601 ttgaatttac agaacctata gaaagtacgc tgtcaccgtc agccggtgca tttaatgtat
      661 tatgagaatt atgtgaaccg ttgtttcctg cagagttgca gactatgatt cctttattta
      721 ctgcaaggtc agctccaatt gtaattacac aactatcgcc gttcatccat gtccagtcgt
      781 aagatcttac cgaaccctga tccattgtca gatagccgag cgaacttgta attatatcag
      841 ctccaagact gtcaacccat tcggcggcag cgatccagtt atcttcttcc agcggagttt
      901 cgctgtcagt attttcagtc tttgcgagaa tatactgcga cctgaatgcc ggcgagatca
      961 gattacccgg actgtatccg cctacaagtg aaagtgttgc tgtgccgtgt gaaccttcac
     1021 ccatctgacc attctgattt ccaactatcg tatcgtgatt tacaaaatca taagatctga
     1081 atcctcttgc tcttaaagaa tcaaatgccg gatgctgaag attgtcaaaa cccgtatcaa
     1141 aatttgcaat gataactccc tgtccgaaat atccgctgtc atgaggaaca gttgcattga
     1201 tgatctccat ctgttccagt gaattcccat aattcagtaa agtagaatta tcattttcca
     1261 aaaaattatt ttcgtgagtt acttcacttg aattattttc aaattcaata ttatcattat
     1321 tttttttata agtcctgacg atatctattt tgcttacaaa ttctttatcc gctaaatttt
     1381 ctatctgaga aatgtctgca tagcatgaaa cgctgttaaa ccattttgat ctgttcttga
     1441 tttttattct caaccgggaa agatcagata tgtaactctg atttaatgga atatctgtat
     1501 agtccaccaa agcatttgca ggcttcactt tcattcttct tttgatcgcc ctttcggtta
     1561 aatatctttc tggatgagca agaagttcat tcacgtcagt acctttgtct ctgaatgtta
     1621 tccagatatg taattcctga ttagcgtcta aagaactcat ttctgaattc agagctttac
     1681 ttattttgaa attatttgag aaattgttgc tgccggtgat gattaggaaa gtgattgcga
     1741 atacaggtat taaaataaat cttaagtata tattagtttt tttcataaag tatttttttt
     1801 gtaagtttac tttttaaaat tatcgatttt aattttaata atctaaatat aattaaatct
     1861 gtcaattgaa ttttgataca aaaagcatta ataataattt tgttcacaaa atatcatgaa
     1921 aaaaaattta gccggttaaa attgtaattt ttaaagttga ataggttttg taaatatgac
     1981 aatgtatttc acttaaaagt cgctgaatta aaatttgttt tcttatcaga ctgaaactaa
     2041 atgcatttga tcttttttat gtccgaaaaa acaaagacac taaaaaattt actttagtgt
     2101 ctctgtaaga taaatgacat tacgatcaac aaaaagaatc tttaaaagtt acggagtcga
     2161 cgccactaca aaattacctg cattattatc cgcatacaaa agatccgcaa tatcagtaag
     2221 tccgtctcca ttcagatctg aatcattata tcctgtaaga aagtcattag catcattatc
     2281 aataatagaa agatcaccaa gatcgacaga cttatcactg tttacatcac ctgaataaaa
     2341 tacccacttc gaacctttca ataacatgtt gtctccaaat gccataaccg ctgctgcagt
     2401 aaaattataa ttcaaaacat ttgaaataaa gctttgagta tttttactcc atgtctcgag
     2461 tgcattcctg tgaaggatct gcagataata atttacaccg ttgtttacat tgccgtattc
     2521 aagagtaagt gtaccgttgg aatttaaaac tcctttctcc tgatcaagaa tattatatgg
     2581 agaggaagaa cttctgatgt tagcagtgac ggtatcagct gccattgtat ttgaatttgc
     2641 atcatataat ccttctatca cagctgtcag atttaatttc tttggcaatg ccagtttgat
     2701 cgcttcccac gtattgatga tcccatatcc gtaaaatctg tcaggtgaat tataattgtc
     2761 agcagacata tataaaattt ctctcacctg aatcggcgtc aggtctggat ttgctgacag
     2821 gatcattgcg catgcacctg ctgtcatggg acatgagaat gaagtcccgc tgccgctgga
     2881 atagcctgtg tttcccgaac cggtctgagc aattttatta ttactgccca tcgccattat
     2941 gtcaggcttg atccttccgt caatagtcaa tcctacagat gaaaaagaag atcttgtcga
     3001 agaagaagtt actgatccga ctgcgatcac actgtctccg tcagccggtg acattatagt
     3061 attatgcgat gcatgactgc cttcatttcc tgctgagttt attactaaaa tacctttatt
     3121 gactgcaagg tcagcagcta tagttaaaaa gcaactgtca ccgttcatcc atgtccagtt
     3181 atatgagtga gatgaaccgt tatccattcc cagatatccg agtgaacttg taattatatc
     3241 agcacctaaa ctgtcagccc attctgcagc ggctatccag ttgtcttctt cgagcggagt
     3301 ctcactgtca gtattttcag tctttgcaat aatgtaatgc gatctgaacg caggagaaat
     3361 gagacttccc ggtttgtatc cgccggccag cgaaagcgtc atagttccat gccagcctgc
     3421 tcccatctgt cccgtctgat cagctacaat agtatcatta ttcacaaaat caaaagtcct
     3481 gatcccgcgc gctcttaatg aatcaaaaca cggatgctcg agattgtcaa ctcctgcatc
     3541 aaaagatgca ataattattc cctgtccgta atatccgctg tcatgtccca tcggagcatt
     3601 tatcatttcc atttgtgtct cagatgtacc atagttcaga gcatactgta aaccgttttg
     3661 attattgttt ccgtcagttt ccaaaaattc atttcccgga ccggactctg catcatcata
     3721 tttttttcta aacttcatga caatatctat tccatttata taatctttgc tgacaatgtt
     3781 atttatctga tctttgtcag catagcatga gatcctgtta aaccatcttg acttttgttt
     3841 tacttcaatt ccggaatttt gcagatcact tatataattt ctgctgacag gaatgtccgt
     3901 caggtcaaca agcttccctg caggctttac ttttttcctt ctgtcgagag atcttttcgt
     3961 gacaagagtt tcagggtcag caagaagtga actgctgttt tcgcctttat ctgtaaatga
     4021 gatccagatg agaacttttt cattctgctg catgtcattt aaaagcaaag agagattttt
     4081 gcttatttta aaattatttg taaagttgtt gctgcctgta agtgacagta taaagattac
     4141 tgccagtgtt aagaaagtta gcttgtagaa ctttaattct tttttcattt taactacaat
     4201 tagtattgct ggttaataaa tctatcatat aatttttgag tatcggattt ggggagttct
     4261 taaaattaag attcgttttt ttaaacttat tataattttt tggcacttac aatttatttt
     4321 ttttactcac aatacaatca gcagtttaat tcactcatat ctaatacatt tctaatagtg
     4381 tttatgaata cattctttat cacttaaatt caaaaattct ttttattaaa tactttcatg
     4441 gaaacaaatc cgggtataaa aatccatgtc ataagaagta taaatgctgt gatcattccc
     4501 actgcacttc caaaaaaatc cctgaacaaa gcgcctgtaa accccatcaa tgctgagata
     4561 tttatctgga gaagaattaa tattctgctg agatctaccg gattcagaga agtaaaaata
     4621 agtatgaatt tctcaagagg gtaatcattg aacgcaaaga gtatcacaaa aattattccg
     4681 tcaaatatca ctgacataaa aagccagatg atcacagaaa gtccgatacc ttttacttta
     4741 tccttattga tcactgatga aaaaaatgcc agtgacacaa aaatgaaagt cagtatcaca
     4801 cctgtgccaa gtaaaaacat taccggcact gtaatgccgt aatatatcag cggcagacct
     4861 gcacctgcaa tgaatgccag agaaagtgag aacgcaattc ccagatattc cgcaaggaat
     4921 atgctttttc tgtcaaccgg ctgtgtaagt aaaagctgta tgaattcttc cgaattataa
     4981 aaatgaatag tcccgaatat taagctgaca agtggaatta taaaaagtat cagattcagt
     5041 aatgttacaa ctgccttgga tgaatcatgt ccgatataaa ttatcgctga gcttaataca
     5101 aataaaaaaa ttgcatagaa tattatgaac cggtttcgga tcaattcaat aaatatatat
     5161 tttaatattt tactgctcat ataattttat ctgttttatt ttgaagaagt cttgaaattg
     5221 ttttccccaa tgtttctccc gaaccgttta tcagatttga gatcttatca ttaaataata
     5281 tttttccttc aatcagataa acaagtctgt cagcaagttc actgacttca ggcaccagat
     5341 gtgaacagac tataaccagc ctcccgaggg atttggattt caggatcttg tcttttaaaa
     5401 tttctgtaga taccggatcc aggctggatg taggttcatc gagaatcaaa atttccggac
     5461 tgaataagaa tgcaatagct gctgtgactc tctgtttata cccgcccgaa agctgaccga
     5521 aatttttatt ttcaatttcc cccagtttaa aattttcaaa aagttcaaca tcatattcct
     5581 ttacatctgt ccggatatct tttacaattt tgaaaagctc tttcactttt agattttctg
     5641 gatagtttgc tatctgcggc atgtatccga tatattttct gtagtcgaat ttatttttaa
     5701 tatcgctgtc tttcacatat atttttccgg aatccggtat tacaagaccg aggatagatt
     5761 ttaggagagt agatttccct gaaccgtttg gacccataag ccctgttatc tgacctttac
     5821 caagattgat atcaatggat ttcagaactt caaatttccc atatctctta tataatttat
     5881 cagttcttat cataaatatt aaattcgtat ttactcattt tcggattctg atctgcgaga
     5941 gattcgggaa tgaagaccgg catagctttt tcagtaatgt cgatcagatc tacaataaag
     6001 cttctcaaca gaataattgc atccggcatt ttttcgagca tcatcgaata tagacttaca
     6061 ggtctgtatg gcacgtcacc cgttccgttt ttatttatgt catatcctcc gtatttgtcc
     6121 cagtaatttg aatcatagaa attactgttt cgtgaactgt tagaagatat gtcaaatgta
     6181 ttttctataa aattatttct ttcaagaaaa ttatccgaac agcttcccat tattttcaat
     6241 gcccagccgt tctttataaa atcattttca aacaaataaa tgtcgttgca tccttccata
     6301 taaataccgg ttgtattttc aataaatatg tttccgaaga tcttacttct tctgatctct
     6361 tttaagagta ttccaaatga tgctgcaccc cagttgtgaa taaaagtatt atgagtcatc
     6421 tctattttct ctgaatacat aacagcaact cccgctccgt tattcttaaa agtattatgt
     6481 gaatatgaat tactgtccgc atacataaag tgcaaaccgt atctaagatt tttttcgctg
     6541 aaattatttc ttacaatcga aaaatcaaca aactccagat agatcccgtc acgctgaccc
     6601 gaaatataat tgttttctat cataacattt ttgcatttcc ataaatgtat tccatttcct
     6661 gatgaagtct ctgtcgcggc atttcctaca atagtatttc ccgatacaag gcagtccgag
     6721 gagttttcaa gatatataga aaaaaatgta tcgaataatc tattgttgaa taccttgctg
     6781 aaatttgatt tttcaagttt tattccggca atatctttta tataactaac gaccatattc
     6841 ttaatggtaa gtccgcttac ggatgaactg tcctttctca atataatacc gtctcccttg
     6901 aaatcaatat caatcaaaga tccttcctcc ccaattatct caaccctttt ttctgctatc
     6961 agatcatact ctttataata accgctttta attaatattc tgtcgccgtc ctcagaaatc
     7021 ctgagtgctt ctttgatcag atttatctgt ccggcagggt ctactgtaat ttcctttgca
     7081 gagatcaatg attccggaat tgaaacaaaa atagctaata gtattattaa tatgattttc
     7141 agagttttac tatttatatt taatttattt tttaatgaac ttttctttaa gacttttaaa
     7201 gtcaaacaat tcaccgccgt tttcttttat aaaattttca gcttcagttt tattactgta
     7261 cgaggcaaga tcaccaccca ttggactttt gatagattca cttaagacaa aaattgcatc
     7321 atcacttttt attaactcac ctggatttaa aatatcaatt gtataagaag tgtataaagt
     7381 aaagatttca ttttcactgt ccgatctttt ttcttcctca tattcaacca tacattctat
     7441 agcatcgaat ttatatgctt tccctttatc atttaccaat tctcccccaa attttttatc
     7501 gactatcgtc attttacagt gatcacacat gtcttttcca aaatttattg gagagagttc
     7561 cttactgcat gatgtaaatg tcagacagat aacaatataa attattgatc ttaatttcag
     7621 atgattatta taaagatttt ttaaagaatt ttttttcatt tatataaaca taaataaaaa
     7681 ttaagccggt cagaaaaaat atatagcttc ctatatccgg cattgaaaca gctttgaaat
     7741 tcaaaagatt tttagatccg ataagcggcg gctgataatt cataccttca acttttatag
     7801 ctgctgttgg atcaagatta tgaccgtaat catattccca cagataaaaa tcataaagac
     7861 ccacagtacc ggctattaca aataaaacta tccatactaa cagaaattta ggtttattta
     7921 gtacacttac aaaaatacct gcggcaataa aaaaacctat gacgtaaggc attacagtta
     7981 gttcttttat tgattccgga tgaatagttt tcatacctat gtaatggtta agaccattta
     8041 ttatatcaag atcaccgctc attttattta tccatatctt cattccaagt ccctccggat
     8101 actggggtgc ctgcaaagaa atatcccaga tcggcacaaa gaaaacacca atgagtgcaa
     8161 gagaaaatac gactattaat attctgctga tttttttcat gattcaaatt aaaaagggtg
     8221 ctttaagcac ccttctgaaa tttataaaag ttactctttc ttttcatcac caagactgaa
     8281 cttgattgga acatcagatc cctgtgcaga tactcttata tatccctgca tttcctgatg
     8341 gagtgctgag caaaaatctg tacagtacat cggcattatt ccaactcttg acggtaccca
     8401 tttcaatgta catgtctcac cgggcattac aagcatttcc gaattattat ttcccattac
     8461 tgcaaatcca tgaggtatat cccagtcctg ttcaaggttt gtcatatgaa aatacactgt
     8521 gtcaccaagt tttacacctt caatattatc cggagtaaag tgtgaccttg tagctgcaaa
     8581 ataaacatgg acaacacttc cgtttctttc aacccgggca tccaaaatac ttttgattgc
     8641 atgaggattt ttattgtctt cgatcttata tattctgatc tgatcttttg tcacaagttc
     8701 tgcaggaata ccctgtgcgt agtgtggctc acctatagtc gggaaatcaa gaaggatccg
     8761 cattttatca cctgatatat caataagctg tgcagactga gtaagctcag gaccggttgg
     8821 aagatatctg tcttttgtca ttttattcaa agcgatcaga tattttcccc atggcttttt
     8881 actgtcgccg ccgggaatgc ttaagtggcc gatggaataa taaaccggaa ttctgtcaac
     8941 tacttgttta gtttcaatat tccattttac tacttcagaa gaaataaatg aagaagtata
     9001 agcaaatcct ttaccatcaa attctgtgtg aagtggtccg agtccgggat cttttacctc
     9061 gcctgacaga catgcttcat aatttagaat tggtattccg cctgcttctc catcagtctt
     9121 attttcggca attgcttttg tgatctttga aaatgcaaag acagggatca cagtagaaag
     9181 tttaccgcct gcagtaatgt attcacccgt cggatctacg tctgctccgt gcggagattt
     9241 tggacatgga atataataag ctgcaccttt gatatcttcg ggtaaaagag tttttacagt
     9301 gtttatcata gttgatgtcg ctgaatgtgt ttcatcgttc cagatgttat gtgcatactt
     9361 tgtgctcatt tcttttccgg ctccaccttt tactatttca tctactttct tataatttat
     9421 tgcaagaata aaatctttgt ctttctgact ggcattgact tcgagaagtg aatttgcctc
     9481 ttcagaatta taacaggtaa agaaaaacca tccgtccgaa ggtccttttc ctgaatgact
     9541 cagatcgtaa tcgtaaggag gagtaatgat ctgataatct atgttcattc tgccggttgt
     9601 cttatcgact tttataaaag acaaagcacc tttaaactct tctttataag atgtaatcgg
     9661 cacgtccctg ttttctaccg gtatactgaa tcttgttcct gctactacat aatccgtatt
     9721 tggagttgta aaaggtgaag agtgatttcc tgatgaatta gggatctcaa ttatttcagt
     9781 agttttgaat gtcttaagat caattctcgc tattctgggt gtattatttc cgtttatgaa
     9841 aacccacctg ccgtccggaa caccatcagt ctgagaaagc tctgaatggt gagcatcatc
     9901 ccagggaata aatccatatg atgtgttcag catcggcttt gattcctcag tataacccca
     9961 gccgttttcc ggattcactg aaaatacagg tatcactttc agcattctgc ctgaaggaat
    10021 accatatact gacatctgac cactgaatcc gcctgacata aaataataga attcatcata
    10081 ctttcccggc tctatataaa cttttgaagc ttcatctcca ctcatgctaa gtgtctttga
    10141 atctttttcc gagcaggaat agaatgacgc tgcaattatt aaaattaaaa ttcccttaaa
    10201 tattgacttt gagagtgtat tagttttctt cattttttta cctctgattt atatttttta
    10261 gttgttccat tgattgtaaa attttatttg tcgttatttc taaaatattc cagtattgct
    10321 cttgcatcat cctgtgacac attttgaaat gtcattggag ttggatactg tccggatgtc
    10381 gccagtgttt ttgcaatcat attttcctga accatctgta caggattcag tatcatattc
    10441 atgatccatt ccggtttcct tctttttgta acatctttta atggaggtcc gaccagttta
    10501 gagttgatct gatgacaggt tatgcatttc atatcaaaaa tttgtttccc tgtagcaaca
    10561 agattttcat ttattgccgg ttctagtttt atttccttta caggaccaat ccctaactcg
    10621 ggatcattcc caccacaccc tgtatatgaa atcatagtta ttataaaaat taaagcaagt
    10681 atcgagctgt tttgtttatt catttttttg atgttttaat tctgtttaaa taattttttg
    10741 agttattttt taataatttt ttttcggatt taactgcttt taggaaaagt atattattct
    10801 caaggtgaaa atgaagatgc agatctattt caaagtctct aagcatttta taaaataaat
    10861 cttttttatc attacctgcc cccccccctg ttttcttccc ggtttgttca gttttgaaat
    10921 tatcacattc tttttttatg tcagaaaaat tttcaaatgc agtctcatgt tcttttatta
    10981 acactttcac aggacttaaa attgaaccaa acggaggaat gtcgtatttg cttttattta
    11041 aatcaaattc atttattttc tttatctccg ggaatataaa tttttcttct ttctgcatat
    11101 gaatctggaa gtcataactc attatcttca gcttgtcaga tatatccgat ctgattattc
    11161 ccctctttac cagatctaca gctgatctga ttattaatgg aaaaattttt ctgatgtatc
    11221 tgtgatggtt tgcgattatg tagtcgcaca taaaatcaac agtccaatca gaaaatttca
    11281 tgtatcccgc agtaaatgaa ttcagcctgt taaattcatt tataaatttc tcaggtctta
    11341 cattgttctc ctttaagata tttcctaatg tagaatttcc ttttatacaa taattgaaat
    11401 tatacttttc aaacagatct attgcacaga aattataatt gataatgtcc gtcagtcttg
    11461 tatttataat cttatccaaa gttttcataa gtttttctta tacaaattta cgtatatcac
    11521 tggaagaaac ctttgactta agtcaataca tagaagtttt tttgatttaa agatttgtga
    11581 taattctaaa aatattattt ttttaaaaat taccgttgtt attgaaggat tttataaaaa
    11641 tcttttttta aaatttcgaa gaatatttta cgaaaaataa cagttgccgg ggtgttaacc
    11701 tattgcagtt ttttgaagag ttgatatatc taaaatttgt atcctttttg cttttactat
    11761 aataacatta ttatcctgaa gttttttaag acatcttgat aatgtctcat caatagtgcc
    11821 taaatatgcg gcaaggtcat gcttacttat tttaagttct attattcctt tatttttttc
    11881 gtctgaagaa tttaactcat ccagtaaata ctttgccagt ctcactgaaa ctccctgaga
    11941 taaactttca atatgattat ttaaatgccg aagttttttt gaaagtcccg atagcatttt
    12001 gaagcaaatt gttttattct tttcaagaag attataaaat atatctgacc tgatcagcat
    12061 tagcctggta ttattttcaa tcgccattga attggcagga tatgtaaatt cttctctgcg
    12121 gattttatca taatgctcaa ataacggagc ttcagcaaat gtatttgttg ggaaaataaa
    12181 atggataaca tgctctcttc cgtcttttga tattttaaag atctttacac ctccttctat
    12241 tactatataa aatccaatgt atggctcagt atcaaaaaaa atcatctcac ctttgtcata
    12301 tgtcttaatg ctggaatgag agtaaatttt atccatctca ttttcatcaa gctctgaaaa
    12361 caaatttata ttttttagaa tactgctatc catattttaa cttcattatc aacgttgccg
    12421 atacaaaaat aaatgaaata gaatttgcgg caagtaaagg tatgtcagag atcatagagc
    12481 cgtatattat ccagactact actcctatct caagcatgat cagcattggc agtgaaatat
    12541 ccgctgtatg ctttgtcctt aatgctctta atgactgagg cagaaatgca aaagtcgtca
    12601 gactcgctgc tataagcccg gtgataagta caggatccaa atttaaattt taaattttaa
    12661 atattaaata aaaaatttgt ttgttaacgc agagtcacat gatcttcgga ctctgcggat
    12721 aataaaatat actttgaaaa ttaaacattg tacattgtac atagtacatt gtacattaaa
    12781 aaattagatc tttgatttca aatattcatt aagattatcc ttttggatcc tctcctgctc
    12841 catcgaatcc ctgtgtctta ttgttacaga attatcagtc aatgtatccg aatccacagt
    12901 tatacaaaac ggcgttccgc attcatcctg tcttctgtac cttcttccta ctgcacctga
    12961 gtcatcataa aatacattaa aactttttct cagatcatct gtgatattat gcgcaatgtc
    13021 ctgcattccg tccctgttta caagcggaaa aattcctgct ttgaccggtg caagtgaagg
    13081 atgaaatcta agcacagtcc ttgtatccga agatgcagta ccatcagaat tttcctttgt
    13141 aagctcttct tcctcataag cattgcaaag aaatgcaaag aaacttctcg atgcacctgc
    13201 tgaagtttct atcacaaacg gcgtgaatct ttcctttgtc tgatcatcaa agtattcaat
    13261 tttcttgccg gaaaattccg tatgtctctt cagatcaaaa tcagttctgt tgtgaatacc
    13321 ctctatctct ccccacccaa aagggaattc aaattcaata tcaacagcag cttttgcata
    13381 gtgtgcaagt ttttcatgct catgaaattt gatcttgtcc ttatccattc cgtattttat
    13441 aaaccagtta tatctctgat ctttccagta gtcataccac tttgcatcat ccgccggatt
    13501 tacaaagaac tgcatttcca tctgctcgaa ctctctcgtc ctgaaaagga aattctttgt
    13561 attgatctca tttcggaatg ctttccctat ctgcgcaata ccaaatggaa ttttctgtct
    13621 cgaagattct cttacattat taaaatttac atatattccc tgagctgtct caggtctgag
    13681 gaatactgcg tttgatgatt cttccagcgg tcctacatga gtcttgaaca tcagattgaa
    13741 atttctcgcc tcagtaaatt tcccttcctc aaaacatttc acagctttcc tgcctttgaa
    13801 tcctttattg ccgcagtccg attcttctaa ttgatcagct ctgaatctgc ctttgcattc
    13861 tttgcagtcc accatcggat cagaaaaatg ttcgacatgt ccggatgctt cccatactcg
    13921 cggatgcatt aatattgatg aatcgatccc ttctatgttt tcacgatagg tcatttcttt
    13981 ccaccaagac tctttgatat ttctcaaaag ctccactccc aaaggaccgt aatcataaca
    14041 tccattcagc ccgccatata tttctgacga ctgaaatatg aatccccttc ttttcgaaag
    14101 cgaaactatc ttttccgttg catctgattt tgtgttttta ttactcaact tattttaata
    14161 ttaattgatt gaaaattttt taatttgctg actgatatta ttcggtttaa cctcaaagac
    14221 tctaagactc taagactcaa aggctctaag gctctaaggc tctatggctg taaggctgta
    14281 agactgtaag acttaatgac ttaatgac
//
LOCUS       DAORVQ010000080        14163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_1961_length_14163_cov_5.928622, whole genome shotgun sequence.
ACCESSION   DAORVQ010000080 DAORVQ010000000
VERSION     DAORVQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_14163_cov_5.928622"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1976
                     /gene="mrdA"
                     /locus_tag="PLX80_07975"
     CDS             183..1976
                     /gene="mrdA"
                     /locus_tag="PLX80_07975"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQY20761.1"
                     /translation="MELEKRKYIIYTVCFTILALFVVRLAYLQLFSSDELTKESNKNS
                     IKTITITPPRGIMYDRNGKVLVDNKPSYTVTITPNSFDKNNLAELSSVLEISADTLEI
                     ILKSIKGTNRFNPAKIKRDADFRIISYIAENRDRLNGVDYQVEAIRFYPNNFFGSHAF
                     GYCNEISPKKLEAQMGDYYKQGDMIGSNGLESNYENYLRGVKGYKFITVDVKGRELGG
                     LNEGNSDIQPVNGSDLILGMDKDLQEYAEKLISGKQGAIIAVDPRNGEILCFVSKPDF
                     DLSAFAGKTDSKVFSSLLQDPQKPLFNRVVQTKYPPGSTWKMMMSLAALDAGIITTTS
                     TIACPGSFTYGNRSFADHGAYGAINVVRALEVSANVFYYKLGLQVGIDNYYKYGKMLG
                     FGSKTGIDIPNETNGLLPSREYFDKRYGENKWTQGFLVSLGIGQGELGVSPVQMVAYT
                     AAIAMNGLYCQPHFVSKVRNAMTNQEEPVRYEQKRIDLPLKYYEAVKKGMYLVVNGNG
                     TATAIKSPSVVLSGKTGTAQNSSGKNHSWFVGFAPYDDPKIAICVLGEFEGWGSSFGA
                     PTAGALMLKYLGVAGDDPINDIKMVNDGSYD"
     gene            2003..3226
                     /gene="rodA"
                     /locus_tag="PLX80_07980"
     CDS             2003..3226
                     /gene="rodA"
                     /locus_tag="PLX80_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQY20762.1"
                     /translation="MNKGLFERFDSLLLIVTVLVIGCGLTAIFSATYTSGSDYFTKQI
                     IFAITGIVFMILVSYLPPRHLAKASYIIYFLSLILLVLVLFFGKKISGSKSWFSIGSF
                     GIQPSEFAKIATVLALANFLSKSEDGFRNINRLPDFLKAVAIVLLPVGLIMRQPDMGT
                     SLVFLSMLLPILFWTGLSPFALFCILSPAIAVTGAFLGLNYFIVSLVIIGLILLLFKK
                     NVILSAAALALNFVIGYSVDILYNKLAPYQQARIKGVFDPETDPLGSGYNVIQSKVAI
                     GSGGLTGKGFLQGTQTQLKFIPEQWTDFIYCMIGEEFGFIGSVTILVLYMVIILKLVS
                     NANLTKNNFLSVACIGFASIILFHLVINVGMTIGIMPVIGIPLPMMSYGISSLISFLV
                     MIGIGMSAYRYRNQY"
     gene            3286..3819
                     /gene="dcd"
                     /locus_tag="PLX80_07985"
     CDS             3286..3819
                     /gene="dcd"
                     /locus_tag="PLX80_07985"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQY20763.1"
                     /translation="MILSDKQILEEIKNKNIVLKPYDRKCLGSNSYDVHLGEWLAVYK
                     DEVLDAKAHNKVNYFKIPKEGHILHPTKLYLGVTLEYTETYKYVPFLEGKSSIGRLGI
                     DIHATAGKGDVGFKNNWTLEISVKQPVKIYAGMPIGQLIYFKVEGDVLVPYNKKQSAK
                     YNRKTNKPVESMMWKNF"
     gene            3856..4671
                     /gene="pyrF"
                     /locus_tag="PLX80_07990"
     CDS             3856..4671
                     /gene="pyrF"
                     /locus_tag="PLX80_07990"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQY20764.1"
                     /translation="MNFTQKLNYISKKNKSNLVIGLDTDLKKIPDYFLKFENPVSEFN
                     KTVIDATKDLCAGYKLNIAFYEFLEEKGIQAVRESLNSIPDDLIKICDAKRGDLDNTA
                     EMYAAAFFDKYEFDSVTISPYMGTDSVLPFIKRKDKHVFVLALTSNRSSADFELLKAG
                     DKFLYEHVTEKILSAGTEDNIGFVFGANYTSEINEFTAKHPGVSLRIPGIGAQGNDID
                     LLMENIMTENFVINSSRNIIYAAERSDDLNSISELIRSAASKVNESVNKRSKA"
     gene            4766..7306
                     /locus_tag="PLX80_07995"
     CDS             4766..7306
                     /locus_tag="PLX80_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20765.1"
                     /translation="MRYTVILFLFLIYSHSFAQSLNDSALSNISGNVFSEKDNQLSDV
                     NVEISDENSNYTARTNSKGKYSIEIPAGIYDIKVSHPGYSDYLNKNYKLEAGAGKVLD
                     IFLLEKTYSTDEIEVYGIFRQKQDDLRTSLYNIKPGNVKMLPGAVEDVLRTLQTLPGV
                     TSPNDFTSQLVIRGSGPDQNLIVVDNVEVFNPYRLYGLVSMFNPETLSDISLITGGFP
                     SRYGDRLSAVLDVTNREGDPNRDLSSVITASIANANLIFSGKNPFNIPGSWLVSSRRT
                     YYDLIVGPFARNAGLITEDSSFPSFRDIQFKIALGNFNKSKFLINGIFSRDGVDIIPG
                     AENENPDSVAVNDVSRNEMLSAAWQFNPGKNFQSNTTLSWYRNTGDNDFNSELLDPLI
                     DDENYTPEQRDSLRQIGALLGLDFDSRYTFRKYSLSNYNIITQKNSTQEFGAGIDIVR
                     TDLSYNLVVDDQLRSLFQSIPSASAFLNDFNLNGENNTRAFIFGQSMFRAGNDFFIQP
                     SLRLDYFDIIKKPYIAPRINLGYAIDPLTTLRFATGIYYQSPGLEKLVDGRTFYDLKD
                     ADDKDLKAERAYHFILGYDRWITNEWFMKTEGYFKLFENLLEQQVLTGYRYEYTLNDP
                     SNTDPEYVKSYANWTKSSEKLPYDSLTTNPVNIGSGNAYGFEISIEKKYISPKSKLYG
                     WANYSLSFSDRNKYDITTPYRFDQRHTANLVLNYRFNNWLELGARGTYSTNFRYTPPV
                     GITPRLIADSVAVNPLAQEIIFNLDFGTPSNEYSEIKPHYFRLDMRLTAYSNFWNADW
                     AFYLDVINATNRKNVLGYNYYLDENLVINKTVVGMFPILPTIGVSVKF"
     gene            complement(7309..8031)
                     /locus_tag="PLX80_08000"
     CDS             complement(7309..8031)
                     /locus_tag="PLX80_08000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20766.1"
                     /translation="MLKDLINSILKSKGYKIEKIRVKKTNAEGYPKYLEEAAEIGLDV
                     NDYLDAHKGWIKPLPVLEETLFPYIKDLKSPRVMELGPGTGRWSRYIKDHLKTLNCTE
                     YYLVDHSPWFVDFLDKYFSNEKFIKTKQNDGFSLPFENESFDAIFSQGVLLELKLPVI
                     YLYAKEFSRVLTKGGVCVFDYFTYDSEEGWEYFLKATNNGSLFFTYYTDSIIDKIFLQ
                     EGFSLVKRFTYGKSKFPVYKKN"
     gene            8223..9428
                     /locus_tag="PLX80_08005"
     CDS             8223..9428
                     /locus_tag="PLX80_08005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY20767.1"
                     /translation="MSDVFIIDGIRTPVGKFAGALKNSRPDDLAAMLISEIVKRNFSG
                     KDISQKEIEDVILGNANGAGEENRNVARMSLLLAGLPVSVAGVTVNRLCASGMQAFID
                     GVRAVRNGEGKCYLTGGVESMTRAPFVMAKNAEPYGRIADVYDTTIGWRFTNKKLAKL
                     FHPFSMGETAENVAEKYKITREDQDEFAFNSQMKAKAAIESGRFKDEIIPVDVSTRKE
                     NIIFDTDEFPRFDTTIEKLAKLNPAFREGGTVTAGNSSGINDGASMLMLASEEFIKEH
                     NINPRARYISSAVAGVDPACMGIGPVPATNKALIRAGLKISDIDLFELNEAFAAQSLA
                     CISELGLDQSKINVNGGAIAIGHPLGSSGSRIIVTLINELEKRKLKTGLATMCIGVGQ
                     GIATIIELC"
     gene            9472..10062
                     /gene="pgsA"
                     /locus_tag="PLX80_08010"
     CDS             9472..10062
                     /gene="pgsA"
                     /locus_tag="PLX80_08010"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQY20768.1"
                     /translation="MSLPNQLSILRILLAPLFLFLFLSEDQLYKELSIVVFLLAVFTD
                     WYDGWHARKFGLVSKLGIFIDPLADKILTSAAFIGFYFMGFMPIWMVLITVVRDLIIT
                     ILRSYHEIKGITMKTSFIAKTKTFIQMTYIFSIVLLIGIQTYSENIEVKNSINDFLFS
                     NVNYLLMLLVTLLTLITGITYFFEKNNAVNSLNEIS"
     gene            10049..10573
                     /locus_tag="PLX80_08015"
     CDS             10049..10573
                     /locus_tag="PLX80_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HQY20769.1"
                     /translation="MRFLKKRKITDENFKPDIFSVIFSSCFYIGYIPFASGTFGSIFG
                     ILFFLINGFTDPVILIPVIILSFIVGIYTSGVMMKRFGDDPSEVVVDEVIGMWITVLV
                     FLILDPSVASLNSGHYIILFMSFRFFDIFKIQPAKYFDELDTAFGVMMDDVFAGVYAG
                     IVSFFLFMIINNFI"
     gene            10582..11841
                     /locus_tag="PLX80_08020"
     CDS             10582..11841
                     /locus_tag="PLX80_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQY20770.1"
                     /translation="MKAKLISIGDEILIGQIINTNAAFIGERFYSAGIPVLKNTVIGD
                     EENILMEELSDSEMNYDVTIITGGLGPTHDDITKPVLVKYFNDKLISDTKVLEHVRNI
                     FLSRNINMPLVNEAQALIPSNSKVIWNSNGTAPGILIVKDKKVFVALPGVPYEMKPMI
                     DEQVIPFLIKFFDLNNKLIHRQKTLLTTGMGESSLNELLGNISELIGESKLAFLPSSS
                     GVRLRINVTEENESEAEDKLFLIENKIRDKIGEFIFGEGEIDLENVIGQILKERNLKL
                     ALAESCTGGRISSRIVSVPGSSEYYLGGVCTYSNEMKISILGVNEDTLNKFGAVSKET
                     ATELAECVRKITDADYAISTTGIAGPTGGTEEKPVGLVWIGFADRKSSYAQKFLFGNN
                     REINIQRSSQRALDILRRELLGIKTEY"
     gene            11866..12330
                     /locus_tag="PLX80_08025"
     CDS             11866..12330
                     /locus_tag="PLX80_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20771.1"
                     /translation="MSFIKEIYDVTKDLTNNKIALNKIKRALLTESKLNNKILEDISN
                     KENRPDFKRMSAIVKNLETDEIKAANNCGIPMELLTLKKVTPEMLEDLDYIRAEGFDL
                     ETVIEKLFIKIAYLKKDFDSENINLFIRLRNIYSYNLLLQRMLDIKLIKSNG"
     gene            12323..12772
                     /locus_tag="PLX80_08030"
     CDS             12323..12772
                     /locus_tag="PLX80_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20772.1"
                     /translation="MVDNIIIYAGIGFAAAIIISLLIKFLFAKNVETINMTSDLRANF
                     HKGKSLQEQVRSMSDAGNRNDAVEILKNSGKLNNEQSQKLMELIGKLGGNNNTSANAG
                     GNELLEKVKNLLRENKKIEAIKLMKESGIGSLKDAKDIVDKIESTGT"
     gene            12805..13836
                     /locus_tag="PLX80_08035"
     CDS             12805..13836
                     /locus_tag="PLX80_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY20773.1"
                     /translation="MSIVTKVLFHWEIDKVLKDHFKKKFLNNKRVELIFPKDLSDKNL
                     IRLAKDADVIVGWRPSIELLRASEKLKLFINPGTGIKHHIENFRELQKTANVTLVNGH
                     GHAYSTAQHTVAMLLTLMNRIVSHHDQMVNGVWRMSDDKDVMSSSIQLRNRKIGLLGY
                     GAINKYVHRFLSGFENDFNILRYSWDSDKSKFPTPVKKFTSKNINSFLKESDILIIAV
                     PHTVLTENMIKAEEIKLLGSDGILVNVARGTIVNEKDLYDSLKKKVIAGAALDVWYNY
                     SPKKNKDGKAFPSSKPFGKLKNVVLSPHRAASPFDEISRWDEVIINILKVSKCKSDYL
                     NIVDLDKEY"
BASE COUNT         4861 a         2152 c         2624 g         4526 t
ORIGIN      
        1 ctgtactgtt gttgcttaca gtatattttt ctggatctat tatcagggtt ctacaatttc
       61 ttttcctgaa atctttatga aatatgtttt aacgactaca atttatactc tgttatttgg
      121 aacagctttt attttgtttt caaatgtaat cgaaactcaa aaaaaataat tttaatatac
      181 tattggaact agagaaacgg aaatatataa tttatactgt ttgttttact atactggctc
      241 tgtttgtagt aagacttgca taccttcagt tattctcatc tgatgaactc acaaaagaat
      301 caaataaaaa ttctataaag accattacca ttacacctcc cagaggaata atgtatgaca
      361 gaaatggaaa agtattagtt gacaataaac cttcttacac tgtaactatc actccaaatt
      421 cttttgacaa aaacaatctt gcagaattat cttcagtgct tgaaatttct gctgatactc
      481 ttgagataat actgaagagt atcaaaggta ccaacagatt caatcctgca aagatcaagc
      541 gtgatgcaga cttcagaatt atttcttaca ttgctgaaaa cagagaccgt ctcaacggtg
      601 ttgattatca ggtggaagcc atcagatttt atcccaataa tttctttgga tctcatgcat
      661 ttggttactg taatgaaatt tcacccaaaa aactggaagc acagatgggc gactattata
      721 aacaaggaga tatgatcggt tcaaacggac ttgaatcaaa ttatgaaaat tatctcagag
      781 gtgtcaaagg ttacaaattt ataactgtag atgtaaaggg aagagagctc ggaggtctga
      841 atgaaggtaa cagtgacata cagcctgtca atgggtctga tcttattctt ggaatggata
      901 aagatcttca ggaatatgca gagaaactga tcagcggtaa acagggagcg atcatagctg
      961 ttgatccgag gaacggtgag atactttgtt ttgtctcgaa acctgatttt gatctttcag
     1021 catttgcagg caagactgat tcaaaagtct tttccagtct tcttcaggat cctcagaaac
     1081 cattattcaa cagagtggta cagactaaat atccgccagg ctcgacatgg aaaatgatga
     1141 tgtcacttgc agcacttgat gcgggaataa ttactaccac ttccacaata gcctgtcccg
     1201 gatcttttac ttacggaaac agaagttttg cagatcatgg agcttatggt gcaataaatg
     1261 ttgtacgtgc tctcgaggtt tcagcaaatg ttttttatta taaactcgga ctgcaggttg
     1321 gaattgataa ttattataaa tatggcaaga tgctggggtt tggatcaaag acaggtatag
     1381 atattccaaa tgaaacaaat ggtttgcttc cttcacggga atattttgac aaacgttatg
     1441 gtgaaaataa atggactcag ggatttctag tcagtctcgg aatcggacag ggtgagcttg
     1501 gagtatcacc ggtacagatg gttgcatata ctgctgcgat tgcaatgaac ggactttact
     1561 gtcagccgca ttttgtttca aaagtaagga atgctatgac aaatcaggaa gaacctgtta
     1621 ggtatgaaca gaaaagaatt gacctgccat taaaatatta cgaagctgta aaaaaaggaa
     1681 tgtatcttgt ggttaacgga aacggaactg caacagctat taaatcacct tctgtagttt
     1741 taagcgggaa gaccggtact gcacagaatt cttccggtaa aaaccattct tggtttgtcg
     1801 gttttgcacc atatgatgat ccgaagattg ccatttgtgt gcttggagaa tttgaaggct
     1861 ggggatcttc tttcggcgcg ccgactgcag gtgctttaat gttaaaatat cttggagtag
     1921 caggtgatga tccgataaat gatattaaaa tggttaatga cggatcatat gattaatgaa
     1981 attataatat tccacagtac aaatgaataa aggattattt gaaagattcg acagtctttt
     2041 attaatagtc acagtattgg taatcggatg cggtctgaca gctatattca gtgctacata
     2101 tacaagcggt tcagattatt ttaccaaaca gattatattt gctatcacag ggattgtctt
     2161 tatgatcctc gtcagctatt taccccccag acatcttgct aaagcttcat acataattta
     2221 tttcctgtca ttaattctgc ttgtacttgt actttttttt ggaaaaaaaa tatccggatc
     2281 caaaagctgg tttagtatcg gtagctttgg aatacaacct tcggagtttg caaaaatagc
     2341 aacagtactt gcgcttgcaa attttttatc aaaatcagaa gacggtttca gaaatataaa
     2401 ccggctgcct gattttttaa aagctgtcgc tattgttttg ttaccggtcg gtcttataat
     2461 gagacagccg gacatgggaa catcgcttgt gtttttatca atgctgcttc ccatattatt
     2521 ttggacagga ctttcaccat tcgctttgtt ttgtatcctt tcacctgcca tagctgtaac
     2581 aggtgcattt ttgggattaa attattttat tgtttcactt gtaattatag gtctgatact
     2641 ccttttattt aaaaaaaatg tcatactctc agccgctgca cttgccttga atttcgtaat
     2701 cggatattct gtagacatac tttataataa acttgcacct tatcagcaag cgaggatcaa
     2761 aggtgtattt gatccggaaa cagatccgct cggcagcgga tataatgtaa ttcagtcaaa
     2821 agtcgcgatc ggttcgggag gacttaccgg caaaggattt ttgcagggta cacaaacaca
     2881 gcttaaattt atacctgagc agtggacgga ttttatatac tgcatgatcg gagaagaatt
     2941 tggatttatt ggatcagtta caatacttgt tttgtatatg gtgataattt taaaacttgt
     3001 aagtaatgcg aacttaacta aaaataattt tctaagtgta gcatgcattg gatttgcttc
     3061 tataatttta ttccatcttg tgatcaatgt cggtatgaca attggtataa tgcctgtgat
     3121 cggaattccg cttccaatga tgagctatgg aatttcctca ctcatctctt ttcttgttat
     3181 gattggaatt ggaatgagtg cttacagata cagaaatcaa tattaaatat taaattttaa
     3241 atattaaatt ttttaagtta aaaatttttt taattagaaa aacaaatgat attatcagac
     3301 aaacagatcc ttgaagaaat aaaaaataaa aatattgtat taaaacctta tgacagaaaa
     3361 tgtctgggtt caaacagtta tgacgttcat ctgggagaat ggcttgctgt atataaagat
     3421 gaagtccttg atgcaaaggc acataataaa gtaaactatt ttaaaatacc caaagaaggt
     3481 cacattcttc atccgaccaa attatatctt ggcgttacac ttgaatatac agagacatat
     3541 aaatacgtac catttctaga aggaaagtcc agcattggca gattaggaat tgacattcat
     3601 gcaactgccg gtaaaggtga tgttggattt aaaaataact ggacacttga gatctcagta
     3661 aagcagccgg ttaaaattta tgcagggatg ccgattggtc agctcattta ttttaaagtg
     3721 gaaggtgatg tacttgtgcc ttataataaa aaacaaagtg ccaagtataa caggaaaaca
     3781 aataagcctg tcgaatcaat gatgtggaaa aatttttaaa aataattctt aatagataat
     3841 tatcagtaat ttaaaatgaa ttttactcaa aagttaaatt atatcagtaa aaaaaataaa
     3901 tctaatcttg tcatcggact tgatacagat ctgaaaaaga ttcctgatta ttttttaaaa
     3961 tttgaaaacc ctgtttctga atttaataaa acggtcattg atgcaactaa agatctatgc
     4021 gccggatata aactgaacat tgccttttat gaattccttg aggaaaaagg aattcaggca
     4081 gtcagagaaa gtctgaattc aattcctgac gatttgataa agatatgtga tgcaaaaaga
     4141 ggtgatctgg ataatacggc agaaatgtat gcagctgctt tttttgataa atacgaattt
     4201 gattctgtca ctatcagtcc ttacatgggg acagactcgg tgctgccgtt tataaaaaga
     4261 aaagataaac atgtttttgt attagctctt acatcaaaca gatcttccgc tgactttgaa
     4321 ctgttaaaag caggtgataa atttttatat gaacatgtaa cggagaaaat actttccgca
     4381 ggtacagaag ataatattgg ttttgtattc ggtgcaaatt atacttcgga gatcaatgaa
     4441 tttactgcaa agcatccggg agtatctctt cgtataccgg gcatcggtgc acagggaaat
     4501 gacattgatc tacttatgga aaatataatg actgaaaatt ttgtgataaa ctcaagccga
     4561 aatattatat atgctgctga aagatctgat gatctgaatt caatctcaga gcttatccga
     4621 agtgctgctt caaaagtaaa tgagtctgtt aacaaaagat caaaagctta gctgattctg
     4681 tgattcagaa aaaagtttat tgaaacttat tcaagctccg gttagttttc tcatctgtgt
     4741 tccaaattca tacctataaa atttaatgag atatacagta atattatttc tatttttaat
     4801 atattcacat tcatttgctc aatcattaaa tgattcggct ttatcaaata tttcaggaaa
     4861 tgtattcagt gaaaaagata atcagctctc tgatgtaaat gttgaaatct cagatgaaaa
     4921 tagtaattac actgccagga caaattcaaa aggtaaatac agtattgaaa taccggcagg
     4981 tatttatgat ataaaagttt ctcatccagg gtacagtgat tatttaaata aaaattataa
     5041 attagaagca ggtgccggga aagtgctgga tatttttctt cttgaaaaaa catacagtac
     5101 tgacgagatt gaagtgtatg gtatctttag acaaaagcag gatgatcttc ggacaagtct
     5161 ttataacatt aaacccggaa atgttaaaat gctgcccggt gcagtagaag atgtattaag
     5221 aactctgcag actctgcccg gcgtgacctc accaaatgac tttacttcac agcttgtaat
     5281 aagaggctca ggacctgatc agaatttaat tgtcgtggac aatgtagaag tatttaatcc
     5341 atatagattg tacgggcttg tcagtatgtt taatcctgaa acactcagcg acatctcact
     5401 tatcacaggc ggttttcctt ctcgatatgg tgatagacta tctgcagttt tagatgttac
     5461 aaacagggaa ggtgatccta acagagatct ttcatcagta attactgcaa gcattgcaaa
     5521 tgcaaattta attttcagtg gtaaaaaccc attcaatata cccggctcat ggcttgtttc
     5581 ttcgagacga acatattacg atcttatcgt cggtccgttt gcaagaaatg ccggtctgat
     5641 aactgaggat tcttcattcc cttcattcag ggatattcaa tttaagatag cattgggaaa
     5701 ttttaataaa agtaaatttc tgataaatgg tattttttcg agagacggtg tagatattat
     5761 accgggggca gaaaatgaaa atcccgacag tgtcgcagta aatgacgtct ccagaaatga
     5821 aatgctttct gctgcatggc agtttaatcc cggtaaaaat tttcaatcga acactacttt
     5881 atcttggtac agaaataccg gtgacaatga ctttaacagt gaacttttag atcctctgat
     5941 tgatgatgaa aattatactc cggagcaaag ggacagtctc agacagatcg gtgctttgct
     6001 cgggctggat tttgactcaa gatatacttt cagaaaatat tcattaagta attataatat
     6061 aattactcaa aaaaattcta cacaggaatt tggtgcgggg atagacattg tcagaacaga
     6121 tctgtcttat aatttggttg tagatgatca gctgagatca ctgtttcaaa gcataccatc
     6181 tgcttcagct tttttaaatg attttaattt gaatggtgaa aataataccc gtgcctttat
     6241 ctttggacag agtatgttca gagcaggaaa tgattttttc attcaacctt ctctgaggct
     6301 ggattatttt gatattatca agaaaccata tattgcacca agaattaatc taggctatgc
     6361 aattgatcca ctcacaactt taagatttgc tacgggaatt tattatcagt caccgggtct
     6421 ggaaaaatta gtagatggaa gaacttttta cgatctgaaa gatgcagacg acaaagacct
     6481 gaaagcagaa agagcttatc attttattct tggttatgac agatggataa caaatgaatg
     6541 gtttatgaaa acagaaggat acttcaaact tttcgaaaat cttcttgaac aacaggttct
     6601 taccggatat agatatgaat atactttaaa tgatccttcc aatactgacc ctgaatacgt
     6661 taagagctat gcaaactgga caaagtcctc tgaaaaactg ccttacgatt ctcttacaac
     6721 taacccggta aatatagggt ctggaaatgc atatggattt gaaattagca ttgagaaaaa
     6781 atatataagt cctaaaagta aactttatgg ctgggcaaat tattctttat ctttttcgga
     6841 cagaaataaa tacgatatta ccacacctta cagattcgat cagagacata cagcaaactt
     6901 agttttgaat tacagattca ataactggct tgagctgggt gctcgcggaa cttattccac
     6961 caatttcaga tatactcctc ccgtcggcat tactccaagg ttaatagccg attcggttgc
     7021 agttaatcct cttgcacagg aaattatttt taatcttgat tttggaacac catcaaatga
     7081 atacagtgaa ataaaacctc attattttag attagacatg agacttaccg cgtattcaaa
     7141 tttctggaat gctgactggg ctttttatct tgatgttata aatgcgacaa acagaaaaaa
     7201 tgtacttggt tacaattact atcttgatga aaatcttgtg ataaataaaa ctgtggtcgg
     7261 gatgtttcca atattgccta ctatcggagt aagtgtaaaa ttctaaattt aatttttttt
     7321 ataaaccggg aatttcgatt tgccgtatgt aaatctttta accaaactaa atccttcctg
     7381 caaaaaaatt ttatcaataa ttgagtccgt ataatatgtg aaaaacaaac ttccattgtt
     7441 agttgctttt aaaaaatatt cccagccttc ttcactatca tatgtgaaat aatcaaaaac
     7501 acatacacct ccttttgtaa gtactcttga aaattctttt gcatataagt atatcacagg
     7561 aagttttaat tcgagaagaa ctccctgaga aaatattgca tcaaaacttt cgttttcaaa
     7621 gggaagacta aagccgtcat tctgtttagt ctttataaac ttttcatttg aaaaatattt
     7681 atctagaaaa tctacaaacc atggtgaatg atcaacgaga taatattctg tacagttcag
     7741 tgtttttaaa tgatccttta tatatctcga ccatctgccg gttcccggac cgagttccat
     7801 tactcttgga gatttaagat cttttatata aggaaacagc gtttcttcca gcacaggcaa
     7861 cggcttgatc catcctttat gagcatccag ataatcattt acatccagac caatttccgc
     7921 agcttcttca agatattttg gatatccttc agcgttggtt ttttttactc tgattttttc
     7981 aattttgtaa cccttacttt ttaaaatgga atttatcaga tctttcaaca ttttttatat
     8041 tttggttaaa agtatagaat ttgatttgaa gattgtattc tatttttttt ataattgcaa
     8101 aaataataat gactaaaaaa tttcatgcat taaatttata tgcagtatta aattaatttt
     8161 aaaagtatct ataattgttt aatttgaatc taaatattaa ttaaacagaa aggatttatt
     8221 ctttgagtga tgtatttata attgacggaa tcagaacacc tgtcggaaag ttcgcaggtg
     8281 cattaaagaa ttccagacct gatgatcttg ctgcgatgct gataagtgaa attgtaaaaa
     8341 gaaattttag cgggaaagat atttctcaaa aagaaattga agatgtaatt ttaggaaatg
     8401 caaatggtgc aggggaagaa aatcgtaatg ttgcaagaat gagtttactt ctggcaggtc
     8461 ttccagtttc tgtagcaggt gtaacagtta acagactctg cgcttctgga atgcaggcat
     8521 ttattgacgg agtaagagct gtaagaaatg gagaaggaaa atgttatctg acaggaggag
     8581 ttgaatcaat gacaagagct ccgtttgtaa tggcaaaaaa tgcagaacca tacggcagga
     8641 ttgctgatgt ctatgataca acaataggct ggagatttac aaataaaaaa ctggcaaaat
     8701 tatttcaccc tttttcaatg ggtgaaaccg cagagaatgt agcagagaaa tataaaatta
     8761 caagagaaga tcaggatgaa tttgcattta attcacaaat gaaagctaag gcagctattg
     8821 aatcaggaag attcaaagat gaaattattc ctgtagatgt atctacccga aaagaaaata
     8881 ttatctttga tactgatgag tttccaagat ttgatactac tatcgaaaaa cttgcaaagc
     8941 tgaatcctgc tttcagagaa ggcgggacag tcacagcggg aaactccagc ggcattaatg
     9001 atggtgcaag tatgctgatg ctggcttctg aagaatttat aaaagagcat aacataaatc
     9061 caagagcaag atacatttct tcggcagttg caggtgtgga tccggcgtgt atgggaatag
     9121 gtcctgtgcc ggctaccaat aaagcgctga taagagccgg tctgaagatc agtgatatag
     9181 atctgtttga actaaacgaa gcatttgcag ctcaaagtct tgcctgcatt tcagaacttg
     9241 gattggatca gtcaaaaatt aatgttaacg gaggagcaat tgcaattggt catccgcttg
     9301 gcagcagcgg ttcaagaatt attgtgacac ttattaacga acttgaaaaa agaaaactaa
     9361 aaacaggcct tgctactatg tgtattggtg tcggacaggg aattgctaca attattgagt
     9421 tatgctgatc atgcattatt tgcaataaga ttttaaatcc attaaagata aatgtcttta
     9481 ccaaatcagt tatcaattct tagaattcta ttagcacctt tattcctgtt ccttttttta
     9541 tcagaagatc agttatataa agagctgtca atagtagttt ttctgctggc agtttttaca
     9601 gactggtatg acggatggca tgcaagaaaa ttcggactgg taagtaaact cgggatattc
     9661 atagatccgc ttgctgataa gatccttact tctgctgctt ttataggatt ttattttatg
     9721 ggttttatgc caatttggat ggtcttaata actgtagtca gggatttaat aattacaata
     9781 ctaagatctt atcatgaaat aaaaggcatt actatgaaga cttcttttat agcaaagaca
     9841 aaaactttta ttcagatgac atatatattt tcaatcgtat tgcttatcgg aattcaaact
     9901 tattctgaaa acattgaagt gaaaaattca atcaatgatt ttctgttttc taacgtgaat
     9961 tatttactaa tgcttttagt cactcttctg actctgataa ccggaattac atattttttt
    10021 gaaaagaata atgctgtaaa cagtttaaat gagatttctt aaaaaacgaa aaattacaga
    10081 tgagaatttt aaacctgata ttttcagtgt gattttttct agttgctttt atatcggata
    10141 cattcctttt gccagcggta cttttggaag tatctttgga atattatttt tcttgattaa
    10201 tggattcact gatcctgtta ttttaattcc tgtaattata ttaagtttta tagttggaat
    10261 ttatacttca ggtgtaatga tgaaaagatt tggtgatgat ccttcagaag tagtagtaga
    10321 tgaagttatt gggatgtgga tcacagttct tgtatttcta attctagatc cgtcagttgc
    10381 ttctttaaac tcaggtcatt atataatatt atttatgtca ttcagatttt ttgatatttt
    10441 caaaattcag ccggcaaaat attttgatga acttgatact gcttttggtg taatgatgga
    10501 tgatgtgttc gcaggagtat atgcagggat tgtttcattt tttctgttta tgataattaa
    10561 caattttatt tgatcaaaat tatgaaggca aaattaattt caatcggaga tgaaattcta
    10621 ataggtcaga taattaatac caacgctgct tttataggcg aaagatttta ttctgcaggg
    10681 attcccgttt taaaaaatac agtgatcggc gatgaagaaa atattctgat ggaagagctt
    10741 tccgattcgg agatgaacta tgatgttact ataatcacag gcggactcgg acctactcat
    10801 gacgatatta ctaaacctgt tcttgttaaa tattttaacg ataaacttat ttcagatact
    10861 aaggttcttg aacatgttag aaatattttt ttatcaagga atataaatat gccgcttgtt
    10921 aatgaagcac aggcactgat cccttcaaat tcaaaagtta tatggaattc aaacggaaca
    10981 gctcccggca ttctgattgt gaaagataaa aaagttttcg tggcactacc cggagttccg
    11041 tatgaaatga aaccaatgat agatgaacag gtaattccat ttctaataaa attttttgac
    11101 ctgaataata aattaataca cagacaaaaa actttactta caacaggaat gggggagtcg
    11161 agtctgaatg aactgcttgg taatatttcg gaattaatag gagaatcaaa actggcattc
    11221 cttccttcat catccggagt tagactgaga ataaatgtga cagaagaaaa tgaaagtgaa
    11281 gctgaagata aattattttt aatagaaaat aagataaggg ataagatcgg tgaatttatt
    11341 tttggggaag gtgaaattga tctggaaaat gtcattggtc aaattttgaa agaaaggaat
    11401 ttgaagcttg cgcttgcaga atcctgtacc ggcggcagaa tttcctcgag aattgtaagt
    11461 gtccccggaa gttctgaata ttacttgggt ggcgtctgta cttattccaa tgaaatgaaa
    11521 atcagcatac tcggtgtaaa tgaggataca ttgaataaat ttggtgcagt cagtaaagag
    11581 acagccactg aacttgctga atgtgtcaga aaaattacag atgcagatta tgcaatttca
    11641 actacaggta tcgccggtcc aacaggtggc actgaagaga aacctgtggg acttgtgtgg
    11701 attggttttg ctgacagaaa aagttcatat gcacagaaat ttttattcgg gaataacaga
    11761 gaaataaata tacagagaag ttctcagaga gcattagata ttttgagaag ggaattactt
    11821 ggaattaaaa cggaatatta aattcaataa tttagattat atagtttgag ttttataaaa
    11881 gaaatttacg atgttacaaa agatcttaca aataataaga ttgcactcaa taaaataaag
    11941 agagcattgc ttactgaatc aaaacttaat aataaaattc ttgaggatat ttccaataag
    12001 gaaaacagac ctgactttaa aagaatgtct gccattgtaa aaaatctgga gacagatgaa
    12061 ataaaagctg ctaacaactg tggtatacca atggagcttc tgactcttaa aaaggtcact
    12121 cccgaaatgc ttgaagattt ggattatata agggcagaag gatttgatct ggagacagta
    12181 attgaaaaac tatttattaa aattgcctat cttaaaaaag attttgattc tgaaaacata
    12241 aatctcttta tcagattaag aaatatttac agttataatc ttcttcttca aagaatgctg
    12301 gatattaagt taataaaatc aaatggttga taatataata atatatgcag ggattggctt
    12361 tgcagctgca ataataattt cactgcttat caaattttta tttgcaaaaa atgtagagac
    12421 tataaatatg acctctgatc tccgggccaa ttttcataaa ggaaaaagtt tacaggaaca
    12481 ggtgagaagt atgtctgatg ccggtaacag aaatgatgca gtcgagattt tgaaaaattc
    12541 aggaaagtta aacaacgagc aatcgcagaa attaatggag ttaattggaa agctgggtgg
    12601 taataataat acttctgcaa atgcaggagg aaatgaactt ttggaaaaag tgaaaaacct
    12661 gttaagagaa aataaaaaaa ttgaagcaat aaaactcatg aaagagtcag gtatcggttc
    12721 acttaaagat gcaaaagata tagtggataa aatagaatca acaggaactt aaaatttatc
    12781 ataaaaataa attagaattc aattttgagt atagttacga aagtattatt tcactgggaa
    12841 atcgacaaag ttttgaagga tcattttaag aaaaaatttc taaataataa aagggtcgaa
    12901 cttatctttc caaaagacct gtctgataaa aatctgataa gactggcaaa agatgctgat
    12961 gtgatagttg gctggagacc atcgattgaa ttacttcggg cttcagaaaa attaaagctt
    13021 tttataaatc cgggaaccgg cataaaacat catatagaaa atttcagaga acttcaaaag
    13081 accgcaaatg taactcttgt caacggtcat ggtcatgcct attcgacagc acagcataca
    13141 gttgcaatgc tgcttacact catgaacaga attgtaagtc atcatgatca aatggtaaat
    13201 ggtgtctgga gaatgagtga tgataaagat gtaatgagtt cttcaattca attgcgaaac
    13261 aggaaaatcg gtctcctggg ctatggtgca attaataaat atgtacacag atttttatca
    13321 ggcttcgaaa atgattttaa catattgaga tacagctggg atagtgataa aagtaaattt
    13381 ccaacgcctg taaaaaaatt cacatcaaaa aatataaatt catttttaaa agaatctgat
    13441 attctgatca tagctgtccc gcatacagtg ttaacagaaa atatgatcaa agctgaagaa
    13501 ataaaattac ttggcagtga tgggatactt gtaaatgtag caaggggaac gatcgtaaac
    13561 gaaaaagatc tttatgattc attaaagaaa aaagtaattg caggtgcggc attggatgtt
    13621 tggtataatt attctccgaa gaaaaataag gatggaaaag catttccttc gtcaaaacca
    13681 tttggaaaat taaaaaatgt agtcttgtca cctcacagag ctgcgtcgcc atttgatgaa
    13741 atttcaagat gggatgaagt aattataaat attctgaaag tttcaaaatg taaaagtgac
    13801 tatttaaata ttgtagattt ggataaagaa tattaacttg taaaaaaaat tgtaatacta
    13861 tttattatta tttattaatt gaaataactc aaatttaaat ttctcaagaa atttaatcaa
    13921 agggattttt atgaaacgga gtttcattgg aaactggaat ttccaaacgg agtttggaaa
    13981 cgtgcatctt cagactttat tgttaaattt tattaattat taacaattaa caattaacaa
    14041 ttaacaatta acaattaact attaacaatt aacaattaac aattaacaat taactattaa
    14101 ctattaacta ttaactattt agatggatgt atttataatt aacacttcgc tgactattag
    14161 tta
//
LOCUS       DAORVQ010000081        13997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2009_length_13997_cov_6.359991, whole genome shotgun sequence.
ACCESSION   DAORVQ010000081 DAORVQ010000000
VERSION     DAORVQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13997
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_13997_cov_6.359991"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PLX80_08040"
     CDS             <1..1608
                     /locus_tag="PLX80_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20774.1"
                     /translation="ADAYSFASPFDGSVYGNVTIDAGAILNCGNTSGKSLSMFGNVIN
                     NGTLTTTSTGGSIRLKGSSIVNNGTVNPAGSLFFDTTTSISGTGNFTSYANFSGNANV
                     TLNSNHQMHGITLNSNSIFNISNRLLKFTASNPITQNGTFTTTNSTIEYNGTALQSIS
                     TANINYQKLRINNAANTTLPANLTVNDTLSVVLGDLNITSWILTLGPNAYLTETSGNT
                     VFATTGYVTTTRNLNAPSALNVGGMGAVLTTAVNLGSTEIRRGNAVQSGLNGGTSIAR
                     FFDIMPTVNDGLDASMTFKYDDSELNGKPEASLKLFYSTNTGSSWILAGGTPNPGANS
                     ISVSKINSFSRWSADTSSVSMVLNFVMEGFYNPVSNTLNMKDTVRAYFRNTNSPYNIV
                     DSAKSTVDPVTFSAAYNFVNAVNGTYYIQTKHRNSIETWSRSGGEAYSVGNTLKYNFT
                     SSATKAFGNNQIMVDASPVRYGVYAADVNQDGTVDLTDGSLIDNDAANFVTGYVPTDA
                     NGDSIVDLADAVFADNNGLNFVGKITP"
     gene            complement(1771..2694)
                     /locus_tag="PLX80_08045"
     CDS             complement(1771..2694)
                     /locus_tag="PLX80_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQY20775.1"
                     /translation="MSHEIILWIGFNVFILLMLALDLGVFQKKTHVVTVREALIWSVV
                     WILLAMIFNAYIYYDMGEIKAVEFFTGYLLERALSIDNIFVFVLLFSYFKVPAEFQHK
                     VLFWGVLGALILRAALIFLGVVLIHKFAWILYLFGAFLLFTGFRMAMHSDDDIDPEKN
                     IVIRLYKKFFPVTKEYHGGDFFVRNEGKLFATPLFVVLIATEFTDLVFAFDSIPAIFS
                     VTDDAFIIYTSNIFAILGLRAMYFALAGIIDKFHYLKIGLSMILIFIGFKMVIVDLFK
                     IPIGYSLGVIALILILSVVLSIFKTKPDKTI"
     gene            3107..4321
                     /locus_tag="PLX80_08050"
     CDS             3107..4321
                     /locus_tag="PLX80_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20776.1"
                     /translation="MKHVFILFFFFVFSFTLINFQTNVTATEVVPPAYKNTAGNGTFT
                     SPLSNSARTYQLLIHSSQLTDFIGKNLTAISWRNPGNASSSWPVSDVSISSYRILLSG
                     SVDPANRSLAFFADNVVGPQVQVRGGSILFPAGSYSFGSSPNAFGPEIVFDNPYLYTG
                     GNLLIEIRQSGFAGTSRSQDALLASSTPAYGTDFSACWKATDTATVNATQGNFAIVKI
                     STQPTNLNLSLSSFIEGLYNSSTDLMISDTAKVYLHNSASPYAIADSSVSVINSAGLG
                     VFNFNEALDGVNYYIDLRHRNSIETWSATANSFTAGSLSYNMSTNPANAFGSNVIQVD
                     LSPLRYATYSGDVTQDGTVDLSDVSLIDNDAAIFATGYLVTDINGDLVIDVADAVFAD
                     NNAFNFVGKIIP"
     gene            complement(4677..6365)
                     /gene="asnB"
                     /locus_tag="PLX80_08055"
     CDS             complement(4677..6365)
                     /gene="asnB"
                     /locus_tag="PLX80_08055"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HQY20777.1"
                     /translation="MCGIVCVFGNNNNGVTDLRQNVLEMSKKVRHRGPDWSGVYSGGN
                     VIFAHERLAIVDPASGRQPLYSDDKKLILAANGEIYNHKDLRIETPDYNFLTESDCEI
                     ILALFKKKGIDFLDDLNGIFAFALYDEENDCYFIARDHMGIIPLYMGWDKDGNFYVGS
                     ELKALEGYCNKIQEFLPGHYIYSKEGQDMKRWYKREWSEFENVKDNVSDIDKLRDGLE
                     QAVHRQLMSDVPYGVLLSGGLDSSIISAVTKKFSAKRVESDDTMQAWYPQLHSFAVGL
                     KGSPDLAAAQKVADHIGSVHHEIYFTIQEGLDAISDVIYHLETYDVTTIRASTPMYLL
                     ARVIKSMGIKMVLSGEGADEIFGGYLYFHKAPNAQAFHEETIRKLGKLHLYDCLRANK
                     SLAAWGIEGRVPFLDKEFLDISMRLNPKDKMAGEDKETGKVKMEKWILRKAFEDYLPE
                     NILWRQKEQFSDGVGYSWIDTLKEVAEKEVTDEMLANAKFMFPVNPPMSKEEYRYRTI
                     FSSHYPSDSAAKCVPSVKSVACSTPEALAWDASFQNANDPSGRAVKSVHVEAYK"
     gene            6992..8560
                     /locus_tag="PLX80_08060"
     CDS             6992..8560
                     /locus_tag="PLX80_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY20778.1"
                     /translation="MILIIMYLNVNAQLTEQWAKTYNLTGSSSADVSYGIATDNSGNS
                     YVTGSVVNGTQQIDYATIKYNAVGAQEWVRTYNGSSSTSDYASDITVDTEGNIYVTGS
                     VNAATSVTTIKYNPAGDSLWVRIFPNAMCTKIISDKYNNIYVSGRWGDVSFSNYLVIK
                     YNSAGTELWSRKYSSPGSLPDEAIGLNIDNSGNVYLTGRVYVGPFNGIGTVKYDSNGV
                     QKWDAIYYCPDFGSDVGNSVETDNLGNVYITGFSDSSTTGKDIITIKYDSLGVRQWVN
                     RFHDGVEEGLTIRVFDENNIYVSGPSHTSTRPDDFTTLKYNSAGDLIWLRMYNGNNGA
                     DRPFNMILDSTGNIYITGQSQLTGSWEDVSTIKYDPSGALRWSTSYTSPGNNPDTGLD
                     LALNNSGDLFVTGRTYPVGNVQSFVTIKYSQTTGIHQIYGIIPDNYNLSQNYPNPFNP
                     STKIDFQIPENGNVNISLYDISGREIAVLINEVKSAGHYNIQFNGSNLSSGIYFYKIS
                     VNDFFATKKMTLIK"
     gene            complement(9221..11500)
                     /locus_tag="PLX80_08065"
     CDS             complement(9221..11500)
                     /locus_tag="PLX80_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20779.1"
                     /translation="MKKINLIFSFIFLFCFALESNSQNVNVNPGAGSYPTLKDAFDAI
                     NAGTHTGAVTIDIVGNTTETATAVLNSSGTGLASYTSILMTPSGAPRIIEGTLSGATT
                     GSIIRFAGADNVTIDGRIAGTGRNLTVRNNNSTTTTSAAIFLSTVIPSAGDTNGCTRN
                     IIRNLEISCNSPQNLLTTLALTMGITSGLATTGFGSAARNNDDNQYLDNNIVKVRYGI
                     FLGGGVANNLNDNNVIAGNTIGPAAFGADQIGKAGIYVNGQNNCRISCNFIQNVGRDD
                     NVLGLTNGADRCGIGLGQENWSTSTNTTLTGTNNVVNGNIIMNIVETRQFSSAGIICA
                     SSAPVNGGVTNNLISNNVIYTVKANGTSPDQAVGIGYTGGTATINANGDRIVYNSIYM
                     TGLMDPAPGDVSTQNSVGIKIHGLAASSNLTVMNNSIYSDVTSTNPALLHYCISAPSS
                     TYNWGTGGCNNNDYYFVTPTVQMRTGGLGTTTPFASSYATLALWQPVFTVPGAQDGAS
                     IQADPLYTLLPTPYPLIPMALSPLFTAAAPIPGVNFDIYECSDPLSVRGATPTIGAWE
                     YSETLLPVELSSFISNVTGTDVTLNWTTASELNNSGFDIERKNAESGNWSKVGNVTGN
                     GTVSTPQNYSFTDRGLTTGKYNYRLKQIDFNGNFEYFNLSNEVGIGIPENYSLSQNYP
                     NPFNPSTKISYELPMDGMVSLRLFDMAGREVASIVNEVQNAGYYTVNFNASSLSSGVY
                     FYTISSNNFIATKKLMLVK"
     gene            12063..13850
                     /locus_tag="PLX80_08070"
     CDS             12063..13850
                     /locus_tag="PLX80_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20780.1"
                     /translation="MKTLITLLIFLVSVSNVFFNSKEAKATNVIPLAYKSAIGDLSFI
                     GPLANTSRTYQLIIHSNLLTDYLGKKITSLAWRLPASAGSNWPASNVTFSNYKILLSG
                     SVTPANRSLAFFSDNVIGMQTQVRSGSLFIPAGSYPSGSSPNSYGPEIIFDVPYLYTG
                     GNLLIEIRQSGFTGTTQSVDALSSFTGGYNLTYSACWKSSDTATYNAASGNFSIVKLF
                     TAEVVPNDKEFTVGDSQFLGALSFDPKTYQLLIHSNQLTEFIGKKLTAISMRAYIGTS
                     SSWPASDVTFSDYRIYLSGSVDPVNRSLAYFANNITGQQRLVRSGPLVIPAGSYSYGN
                     SPNATGPEIIFNSPYIYNGGNLLIEIRHKGFTGTTLSVDANNFPATGYGTDFSACYKS
                     TDTATFGALAGSFSTVRISSSEPLELNLKAYIQARYSSTANMMVPDTSKVLLRHSFSP
                     YAPADTAAALSGSNGIGSFNFGQALQATAYYFVVRHRNSIETWSANTITFVGNDVSTS
                     IAGNPSQAYGNNLIQVDNGPNAYAIYSSDINQDGTVDLSDGSLIDNDAINFVSGYVLT
                     DNNGDLIVDVADAVFADNNGFNFVIKITP"
BASE COUNT         4586 a         2447 c         2420 g         4544 t
ORIGIN      
        1 gctgatgcat attcctttgc atcgccattt gacggaagtg tgtatggtaa tgtaaccatc
       61 gacgccggtg ccattttaaa ctgtggcaac acatccggaa aatctctttc catgtttgga
      121 aatgtcatca acaatggaac actgacgaca acaagtacag gtggttccat acgtttaaag
      181 ggctcatcaa tagtaaacaa cggaacagtg aaccctgccg gatctttatt ttttgataca
      241 acaacttcca tatccggaac aggaaatttt acttcttatg caaatttcag tgggaatgct
      301 aatgtaacac ttaacagcaa tcatcaaatg catgggataa cattgaattc aaattctata
      361 tttaatattt caaacagact tttaaaattc acagcatcaa atccgatcac acaaaacgga
      421 acttttacaa ctacaaacag cacaattgaa tataacggaa cagcgcttca gagtatttca
      481 acagcgaata tcaattatca aaaattaaga attaacaacg cggcaaatac tacactgccg
      541 gctaatctaa ctgtcaatga cacactctcg gttgttctcg gcgatttgaa tattacttct
      601 tggatattga ctttaggtcc aaatgcttat ctaaccgaga cttcaggaaa cacagtcttt
      661 gcaacaacag gttatgtgac gacaacaaga aatctaaatg ctccttcagc tttaaatgtc
      721 ggtggtatgg gtgcagttct gactacagca gtaaatcttg gaagtacaga aataagaaga
      781 ggtaatgcag tccagagcgg actgaatgga ggaacaagca ttgcaagatt ttttgatatt
      841 atgccgactg taaatgacgg tctggatgca agcatgacat ttaagtatga tgattctgaa
      901 ttaaatggaa aacctgaagc atcattgaaa ttattttatt cgacaaatac aggatcttcc
      961 tggatacttg caggtggaac tccgaatccg ggtgcaaata gtatttctgt cagtaaaata
     1021 aattcattct caagatggtc tgctgatacc agctctgtgt cgatggtatt gaattttgta
     1081 atggaaggat tttataatcc tgtcagcaac actttaaata tgaaagatac tgtaagagct
     1141 tatttcagaa atacgaattc gccttacaat attgtagatt cagcaaaatc cacagtggat
     1201 ccggtcactt tttctgctgc atataatttt gtaaatgcag tgaacggaac atattatatt
     1261 cagacaaagc acaggaattc cattgagacc tggagcaggt caggaggtga agcttatagt
     1321 gttggaaata cgctgaagta taatttcact tcgtcagcaa caaaagcttt tggtaataat
     1381 cagataatgg tggacgcttc accggtaaga tatggtgtct atgcggctga tgtaaatcag
     1441 gacggaaccg tggatctgac agacggcagt ctgatagaca atgatgcagc aaattttgta
     1501 accggttacg tacctactga tgcaaacggt gatagcattg tagatctggc cgatgcagta
     1561 tttgctgata ataatggatt aaattttgtt ggtaaaatta ctccgtaatt aatgttcaat
     1621 attcaatgtt caatattcaa tgaataacat tcaaaattaa ttgtttattt ttaattctta
     1681 actattaatt tttaattctt cacaggctgt gggtttaatt aaaaaattcc tgcagtctgt
     1741 atttttttat ataaaaatgt tacacatgag ttaaattgtt ttatctggtt tagttttaaa
     1801 gattgataaa acaaccgata aaattaatat caatgctatc acacctaagg aatatccaat
     1861 agggattttg aataaatcga caatcaccat tttaaaacct ataaagatca atatcataga
     1921 aagtcctatt tttaaataat ggaatttatc tataatacca gccagtgcaa aatacattgc
     1981 cctcagtcct aaaattgcaa aaatatttga agtataaata atgaaagcat catcagttac
     2041 agaaaaaatt gcagggatcg aatcgaaagc gaacacaagg tctgtgaact ctgttgcgat
     2101 caatacaaca aataaaggtg tagcgaataa tttcccttca ttccttacaa aaaaatctcc
     2161 gccgtgatat tcttttgtta caggaaaaaa tttcttgtaa agccggatga caatgttttt
     2221 ctccgggtca atatcatcat cactatgcat cgccattctg aatcctgtaa ataaaagaaa
     2281 agcaccgaat aaataaagga tccatgcgaa tttatgaatg agcactactc ccagaaatat
     2341 taatgcagcc cttaagatca aagcaccgag tactccccag aataatacct tgtgttgaaa
     2401 ttctgcggga actttgaaat atgaaaacaa aagtacaaac acaaaaatat tatcaatgct
     2461 taatgctcgt tcaagcagat agccggtaaa aaattctact gctttaattt cacccatatc
     2521 ataataaatg taagcattaa atatcattgc caataaaatc cagacaacgc tccagatcaa
     2581 tgcttctctg acagtgacaa catgggtttt tttctgaaag acacccaggt caagcgccag
     2641 catcagcaat ataaaaacgt taaatcctat ccatagaata atttcgtgtg acattcagta
     2701 atttaaaatg attcaaatta aattattatc aatcaattta aaataatata tttataccct
     2761 tttatttgtt attcttaata tagtgaagga taatatttta attcttttta gaaactatat
     2821 ttgcgtttca gtatagatat ggaggttaat tccagttgaa ctgataaatg tatttaaatt
     2881 tacaaaatat tattataagt tcaatctgat taaataaaat aatttttaac acaacaagac
     2941 actaactcta atttctttgg ataaaagatt tgtatgatcc gaaatcaata attgttttaa
     3001 agagaagacc atcaatttga tcttcagtaa aaatcaaagt tcaatatttc caacataaaa
     3061 tatattgaat atttattaaa aaacaattta aaccaaggag gcttcaatga aacatgtttt
     3121 tattctattt ttcttttttg tattcagctt tactctgata aattttcaga caaatgtaac
     3181 agcaacagaa gtagttccgc cggcttacaa aaatacagct ggaaatggta catttacctc
     3241 accattatca aattctgcca ggacttatca gctattgatc cattcaagtc agctaactga
     3301 ttttatcgga aaaaatctta cggctatatc atggagaaat ccgggtaatg catcttcaag
     3361 ctggccggtt tccgatgttt ctatatccag ttacaggata cttctaagcg gcagtgtgga
     3421 tcctgcaaac agaagtctgg cattttttgc agataatgtc gtaggtccgc aggtacaggt
     3481 aagaggagga tcaatactat ttccggcagg ttcatattct tttggaagtt cacctaatgc
     3541 attcggaccg gagatagttt ttgacaatcc ttatttatat accggaggaa atttattaat
     3601 cgagatccga caatccggat ttgccggaac ttccagatct caggatgcac ttctggcatc
     3661 ttctacaccg gcatatggaa cagatttcag tgcctgctgg aaagctactg atacagctac
     3721 agtcaatgct actcagggaa attttgcaat agtaaagatc tccactcaac cgacaaattt
     3781 aaatctttca ctttcatctt tcatagaagg tctatataac agctcgacag atcttatgat
     3841 aagtgatact gcaaaagtat atcttcataa ttctgcctct ccatatgcaa tagctgactc
     3901 ttcagtatca gttatcaatt cagcaggttt aggagtattc aattttaatg aagctttaga
     3961 cggtgttaat tattatatag atctgaggca cagaaattct attgaaacat ggagtgcgac
     4021 tgcaaatagt tttaccgccg gtagtttaag ttataacatg tcaacaaatc ctgctaatgc
     4081 ttttggcagc aatgtcatac aagtcgatct gtctccgtta agatatgcaa catacagcgg
     4141 tgatgtaact caggatggta cagtagatct ttcagatgtc agtctgatag ataatgatgc
     4201 ggccattttt gcaacaggat accttgtgac agatattaac ggagatctgg taatagatgt
     4261 ggcagatgct gtgtttgctg ataataatgc gtttaatttt gtagggaaaa ttattccata
     4321 atctgattta aatttacctt tgtaaatttt gatttgaaat ttttaatatt ttaataaaat
     4381 attttttata aatcgtctgt tgaaaaaaga tcagcagacg atttattata aatatctctt
     4441 tttttattct gagccaatgt cttgccgatt ttgaagttcc caaaaagttt gaataccaat
     4501 tataatgaat tctgtacaaa acatatgtca aacttttaga aacataaatt agtattcaag
     4561 ttacaaaagc cgctaggaaa gatctcagcg gctttacttt ttaaatatag agttcgcaac
     4621 ctaaacgcag cggctacttt atttaatatt taatatttaa aatttaatat ttaaaattat
     4681 ttgtacgctt ctacatgcac cgacttcact gcccttcccg acggatcatt cgcattctga
     4741 aaagatgcat cccatgccag tgcctccgga gtactgcatg caactgactt cacggatgga
     4801 acacatttag cagcggagtc tgaaggataa tgactgctga aaattgttct atatctgtat
     4861 tcttcctttg acatcggagg atttacagga aacatgaact ttgcatttgc aagcatttca
     4921 tctgtgactt ctttttcagc aacctctttt aaagtatcaa tccagctata gccgacaccg
     4981 tctgagaact gctccttttg tctccataaa atattttcag gtaaataatc ttcaaaagct
     5041 tttcgcaaga tccatttttc catcttaact tttcctgtct ctttatcttc tcccgccatt
     5101 ttatcttttg gatttagtct catggaaata tcgagaaact ctttgtcaag aaaaggaaca
     5161 cgcccttcaa ttccccatgc agcaagagat ttatttgcac ggagacaatc atagagatga
     5221 agctttccga gtttacggat agtctcttca tgaaatgcct gagcattcgg agctttatga
     5281 aaataaagat atccgccgaa aatttcatca gcaccttctc ctgagagaac catttttatt
     5341 cccatcgatt ttataactct cgcaagcaga tacatcggag tggaagcgcg aatggtagta
     5401 acgtcgtaag tttccagatg atagatcaca tcgctgatag catcgagacc ttcctgaata
     5461 gtgaaataaa tttcatgatg tacagaacca atatgatctg caactttctg tgcagcggca
     5521 agatcaggtg accccttaag tccgaccgca aatgaatgaa gctgcggata ccacgcctgc
     5581 attgtatcat ctgactcaac tctctttgcc gaaaactttt ttgtaactgc agaaattatc
     5641 gatgagtcta atccacctga aagtaaaact ccgtaaggaa catccgacat aagttgtctg
     5701 tgaactgcct gttcaagtcc gtcacgtaat ttatcaatat ctgaaacatt gtcttttaca
     5761 ttttcaaatt ctgaccattc gcgcttatac catcttttca tatcctgacc ttctttactg
     5821 taaatataat gacccggcaa aaattcctga attttattac agtaaccttc gagagctttg
     5881 agttcagatc ccacataaaa atttccgtcc ttatcccatc ccatatataa aggtataatt
     5941 cccatgtgat ctcgtgcgat gaagtaacag tcattttcct catcatataa tgcaaatgca
     6001 aatattccat tcaggtcatc gagaaaatct attcctttct ttttaaaaag cgcgagaatt
     6061 atttcacagt cagattcagt aagaaaatta taatcaggag tttctatcct gagatcttta
     6121 tgattataaa tttcaccgtt tgctgcgaga attaattttt tatcatcact gtaaagcggc
     6181 tgtcttcctg aagccgggtc tacaattgcc agtcgctcgt gtgcaaagat aacatttccg
     6241 ccgctgtaaa ctccagacca gtccggaccc cggtgacgga ctttttttga catctcgagc
     6301 acattctgtc tgagatctgt tacaccatta ttattatttc caaatacaca tactataccg
     6361 cacataattt aatctgttga tttattttta aaaatttctt taactctgtt gttataatat
     6421 tataatttaa tatatataag gtgagaattg agaacgattt ttttggttga atgcaattta
     6481 cttaattcag aaaaaagtaa caatttagaa tttggtacat agatcaattt tatatattaa
     6541 atattaaata ttaaatatta aattttatat tttggatttt gcctgtttcc attgagccgt
     6601 tgtttttgtc ataaaaaagt ttataaatct aacatggtat ttctgagcca tcaacagcct
     6661 gtgaaagttt tttagcagaa gtgtaggttt tgtaacgctc gcagtgttaa cttagatcta
     6721 caacagtccg aaatctgaga tattaaggtc gcaacctaaa ggatgaggct acgatctttt
     6781 attatgtttt agaaatttcc agagaataag gtaaggcagg ctttgaaaat tcttaaatcc
     6841 taattcctaa tttttaattt tcattaaggt gaaaaacgcc ataaactttc tttgcggaac
     6901 cagtattttt taacgaattt tgtatagaaa aaatttgaat caataattta tttatataaa
     6961 aatgacaaaa agaattaaaa tcacttactt aatgatactg ataattatgt atttaaatgt
     7021 aaatgctcag ctgacggaac agtgggcaaa aacctataac cttacaggga gttcgagcgc
     7081 tgatgtttca tatggaattg ctacagataa ttcaggtaat tcgtatgtga ccggaagcgt
     7141 tgtcaacgga actcagcaaa ttgattatgc gactattaaa tacaatgcag tgggagctca
     7201 ggaatgggta aggacttaca acggttcatc cagtacttct gattatgcaa gtgatattac
     7261 agttgatact gaaggtaata tatatgtaac tggatcagta aatgcagcga cctctgtgac
     7321 tacaataaaa tacaatcccg ccggggattc gctgtgggta agaatatttc caaatgcaat
     7381 gtgtacaaaa attatttcgg acaaatacaa taatatttat gtatccggaa gatggggaga
     7441 tgtatctttt tcaaattatc ttgtcataaa atataattct gccggtaccg aattgtggtc
     7501 ccgaaaatat tcatctcccg gttcattacc tgatgaggca atcggattga atatagataa
     7561 ttcaggaaat gtttatctta ccggaagagt gtatgtagga ccatttaatg gaataggaac
     7621 tgtgaagtac gattcaaatg gtgtgcagaa atgggatgca atttattatt gtcctgattt
     7681 tggttcggac gttggaaatt ctgttgaaac cgataatctt ggtaatgtat atatcactgg
     7741 attcagtgat tcgtctacaa caggtaagga tattattact ataaagtatg attcattggg
     7801 cgtaagacaa tgggtaaaca gatttcatga cggtgtggaa gaaggtttaa caatcagagt
     7861 atttgatgag aataatattt atgtttcagg accgagtcat acatctacca gacctgatga
     7921 ctttacaaca ttaaaatata attctgccgg agatttgatt tggctcagaa tgtacaacgg
     7981 aaataacgga gctgacagac ctttcaatat gatactcgac agtactggaa atatctatat
     8041 taccggtcaa agccagctta caggctcatg ggaagatgtc tcaactatta agtatgatcc
     8101 ttccggagcg ctgagatgga gtacaagtta cactagtccg gggaataatc cggacacagg
     8161 tttggatctt gctttaaata attcaggaga tttatttgta accggaagaa cttaccctgt
     8221 tggaaatgtg cagtcttttg tcacaataaa atattcacaa accacaggta ttcatcagat
     8281 atacggcatc attccggata attataatct ttcacaaaat tatccgaatc cgtttaaccc
     8341 ttctacaaag attgattttc agatccctga aaatggaaat gtaaatatct ctttatacga
     8401 tatttccgga agagaaattg cagtattaat taatgaagtc aaaagtgctg gacattacaa
     8461 tatacaattc aacggttcaa atttatccag cggtatttac ttttacaaaa tcagtgtgaa
     8521 tgattttttc gctaccaaaa aaatgactct gataaaataa aagagaaaca ataacagaaa
     8581 tagaaaatat tctggagatc tcatttatat aaaagatggg atctcttttt tttaaggtac
     8641 gataattttc aaaaaatatt acccaatata taaaatagag gtgctttatg aattcaatga
     8701 aaaacattta gaaaaatatt ttattcacag attctttctt tcccgaataa tccaaatatt
     8761 gtcattcccg cgaattctgg aatctaattt aattagaagc taaggaaatt tggtatttat
     8821 ttactgtcga aatagacaaa gggatttatt tggttaagtt tggaaaaaat gtcaggtttc
     8881 agttgagatt cccgcatccg cgggaatgac tagtgtttgt gggtgttgtc ttgctatttg
     8941 aaatatttct ttgctgatca tttttttgca aagtaaatat ttcttaacac aactttacac
     9001 tataaatcat atagaaccaa aataaatctg ttcatctttc caaaagattg agtatatgcc
     9061 tgagaaagtt ttttagcgga agttggtgtt gttggtcacg ctaaattcgc taactaagac
     9121 atataacggc tcgaatcatt aatccccaaa ataaaagatt ccacttcaat taagaaatgg
     9181 aatcttttgt tttaaattaa aactgtaaaa cagccgatcg ttatttcacg agcatcagct
     9241 ttttagtagc tataaagtta tttgaagaaa ttgtatagaa gtaaactccg cttgataatg
     9301 aagatgcatt gaaattaact gtgtaatatc ctgcattctg aacttcattt actattgatg
     9361 caacttctct tcctgccata tcaaacaatc tcagacttac cattccgtcc attggcaatt
     9421 catagctgat cttagtagaa ggattaaacg gatttggata gttttgtgat aatgaataat
     9481 tttcaggaat accaattcca acttcgttac ttaaattgaa gtattcgaaa ttaccattga
     9541 aatctatctg cttcaatctg taattgtatt taccagtagt caaacctctg tctgtgaatg
     9601 aataattctg aggagtgcta acggttccgt ttcctgttac atttccaacc ttactccaat
     9661 ttccggactc agcattcttt ctttcaatat caaatcctga attgtttaac tcggaagcag
     9721 tagtccagtt aagagtgaca tctgttcctg taacattaga aatgaaagaa gacagttcta
     9781 caggaagtaa tgtttcagaa tattcccatg cacctatagt aggagtagca cctctaactg
     9841 aaagtggatc tgaacattca taaatgtcaa aatttactcc gggaatcgga gctgcagctg
     9901 taaataaagg tgataatgcc attggaatta atggatatgg tgttggaagt agagtgtata
     9961 aaggatcagc ctgtatagaa gcaccgtcct gagctcccgg tacagtaaat accggctgcc
    10021 agagagctag tgttgcgtac gaacttgcaa agggagttgt agttccaagt ccgcctgttc
    10081 tcatttgaac agttggagtt acgaaataat aatcattatt attgcatccg cctgttcccc
    10141 agttgtatgt ggaggatggt gcagagatac agtaatgtaa taaagccgga ttagttgaag
    10201 ttacatctga ataaatagag ttgttcatta cagtaagatt actggaagca gctaaaccat
    10261 ggatttttat accaactgaa ttctgcgttg aaacatctcc tggtgccgga tccatcaaac
    10321 ctgtcatata aattgaatta tatacaattc tgtcaccatt tgcatttatg gtagcagtac
    10381 cacctgtata tccaataccg actgcctgat caggtgaagt tccgtttgct tttactgtat
    10441 agataacatt attactgatc agattattgg tgactccacc gtttactggt gcagaagatg
    10501 cacaaataat acctgcagaa gaaaattggc gggtttcgac aatattcata attatatttc
    10561 cattaaccac attgttagta cctgttagtg tagtattagt ggaagttgac cagttttcct
    10621 gaccaagtcc aataccgcat ctgtctgcac cgttcgtaag tccgagaaca ttatcatctc
    10681 ttcctacatt ttgaataaaa ttacaagata tccgacagtt attctgtccg tttacatata
    10741 tcccggcttt accaatttga tcagcaccaa atgcagctgg accaattgta ttaccggcaa
    10801 ttacattatt atcgtttaaa ttattagcaa ctcctccacc aagaaatata ccatatctga
    10861 cttttacaat attgttatca agatattggt tatcgtcatt gtttctggct gctgatccga
    10921 atccagttgt tgctaaacca ctggtaattc ccatagttaa agcgagagta gttaataggt
    10981 tttgcggcga gttacatgaa atttctaaat ttcggatgat attgcgagtg catccgttcg
    11041 tatcacctgc agaaggaatt acagtagaaa gaaaaattgc agctgaggta gttgtagtac
    11101 tattattatt tcttacagta agatttcttc ctgtaccggc aattcttccg tctatagtta
    11161 cattatcagc gccggcaaat cttattatag aaccggtagt agcgccggat aatgttcctt
    11221 caatgatcct tggagctccc gatggagtca tcaaaatcga agtataactg gctagaccag
    11281 taccgcttga gtttaagaca gctgttgctg tttcggttgt atttcctaca atgtcaattg
    11341 taactgcacc agtatgtgtg cctgcattaa tagcatcaaa tgcatctttt aaagttggat
    11401 agcttccggc gcccggatta acattcacat tttgtgaatt tgattccaaa gcaaagcaga
    11461 acagaaaaat aaagctgaaa atcaaattga tttttttcat ttttgttttt ttgggttatt
    11521 gttgaaatta gttaatttaa tcaggaagat ttaaataaat cactttcata tttttttatt
    11581 ttccttcctt aaaaaaataa aaaaatattt ttttcaatgt aagttattga attttatctc
    11641 ctttctttta tatttttgct ttaaaattat gtatataaat ttaatttcaa tcctaacaaa
    11701 atcctaacaa attaagggta attattacta aaatataaat actttaaggt attattcgga
    11761 aagtttcaga ggaatacgaa aatttaatga atatttaaaa cggaaaatat tttaaaacta
    11821 caaacaataa atttattcat tatagaatta atggtcatat tgacagataa gaataaacat
    11881 tcctcaaagt ctgcataaat attaattatc tattatatca attccaattc ctgaaaattt
    11941 caatagaata ggtgtctgat tataaataca actttgcatt tattatttat tcaaaatggt
    12001 ttattatcta aacactttat aaaattataa atatttaatt attaaaatta cccggatata
    12061 aaatgaaaac actgatcaca cttttaattt ttttagtttc tgtttcaaat gtatttttta
    12121 atagcaaaga agccaaagca acaaatgtaa ttccgctcgc atataaatct gccattggcg
    12181 atctaagttt tataggtccg cttgcaaaca catcaagaac ttaccagcta ataattcatt
    12241 caaatctttt aacagattat ctcggaaaaa aaataacctc tttagcctgg agattacccg
    12301 caagtgcagg ttcaaactgg ccggcttcaa atgttacatt ttctaattac aaaatcttat
    12361 taagtggaag tgtgaccccg gctaacagaa gtcttgcatt ctttagtgac aatgttatag
    12421 gaatgcagac acaggtaaga tcaggatctc tgtttattcc tgctggttca tatccttcag
    12481 gaagttctcc taattcatac ggacctgaaa ttatatttga tgttccatat ctttatacag
    12541 gggggaactt actgattgaa attcgacagt cgggatttac cggcactaca cagtctgttg
    12601 atgctctttc atctttcacg ggcggttata atttaactta tagtgcttgc tggaaatctt
    12661 ctgatacagc gacttataat gcagcttcag gaaatttttc aattgtcaaa ctttttacag
    12721 ctgaagtagt gcctaatgac aaagaattta ctgttggcga tagtcaattt ttaggtgcat
    12781 tatcattcga tcctaaaact tatcagttat tgattcattc aaatcaactg actgaattta
    12841 ttggtaaaaa attaacagct atctcaatga gagcatatat cggaacaagt tccagctggc
    12901 cggcttcaga tgttactttt tcagattaca ggatctatct cagcggaagt gtggatccgg
    12961 taaacagaag tctggcgtat tttgcaaata atattacagg tcagcagaga cttgtcagat
    13021 cgggacctct tgtaattccg gccggttctt attcatatgg aaattctcca aatgcaaccg
    13081 gacccgaaat aatttttaat tcaccgtata tttataatgg cggaaattta ctgatagaaa
    13141 ttaggcataa aggatttacg ggcacaactc tttctgttga tgcaaataat tttccggcca
    13201 caggatacgg tacagatttc agtgcgtgtt ataaatctac agatactgct actttcggtg
    13261 ctcttgctgg tagtttttct actgtgagga tctcttcttc tgaaccattg gaactgaatt
    13321 taaaagcata cattcaagca agatattcca gtactgcaaa catgatggtg ccggatactt
    13381 ctaaagtatt actcaggcat tcattctcac catatgctcc tgctgacact gcagctgcat
    13441 tatcaggttc gaacggaatc ggaagtttta atttcggtca ggctctgcaa gctaccgctt
    13501 attattttgt agtaagacac agaaattcca ttgaaacatg gagtgctaat acgatcacat
    13561 ttgtaggaaa tgatgtaagc acaagtattg cgggtaatcc atctcaggct tatggaaata
    13621 atttaataca ggtggataat ggtccgaatg cttatgcaat ttacagttcc gatataaatc
    13681 aggacggaac agtagatctg tccgacggaa gtctgatcga caacgatgca attaattttg
    13741 tttcgggata tgtattaacg gataataacg gtgacttaat agtcgatgtg gcagacgctg
    13801 tgtttgcgga taataatggg tttaattttg taattaagat cacaccataa tagagcactt
    13861 aaaaattaat aatttgtaat taagaatttt tcacagacag gaaattaaat tttcctgtct
    13921 gttttttttt gtaaattttt taagccttta taaataaatt tggattaaga aaaaatcaat
    13981 atttccttct aatctca
//
LOCUS       DAORVQ010000082        13979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2016_length_13979_cov_6.039572, whole genome shotgun sequence.
ACCESSION   DAORVQ010000082 DAORVQ010000000
VERSION     DAORVQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_13979_cov_6.039572"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PLX80_08075"
     CDS             <1..1232
                     /locus_tag="PLX80_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY20781.1"
                     /translation="YLNSINMKDEKFSNQLEFSKIRNMKILLTALLFLMSLPLFIINT
                     SCKGTQEKITKKKNSEEKTSKVNENDSVIFSFVFVGCNRVDKSEQNNPSATDSSTANL
                     TVLKKICDEITNIQRKPDLFFFLGDLVLGETNTKELDIQLGAWVKLLEDTAFSKISKA
                     DIELVAVPGNHEMLYKKTHKKIKDHDEWPLKDATEIWMKLMHPYLPEDRERITGSDSI
                     IEQMTFSFIRNNVGFIVMNTDTYNEPTETDPYGIEGIIPTAWILKKIKEYRDNPEIEH
                     IFILGHKPYYSYDKPNTGHDGLPEGPVLWPELNKYHVIAMLSAHIHDYLRMQPGNEGT
                     YQIIAGNGGSQGPAEFFGYSIINIMKSGDVQLISKGFDKGDPYYTLPDDEPSTVRDES
                     ILSWTKNSNPYKNPKKK"
     gene            1246..1629
                     /locus_tag="PLX80_08080"
     CDS             1246..1629
                     /locus_tag="PLX80_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY20782.1"
                     /translation="MEKRKLKNNILFEKIDITTGKLDEMVKFYEDQFGIEFEEIVFEM
                     DKKKWKRYLGNLGGIVFHLVPNETSNTEKDQTGVHQFHILVKDIDKFKNSAFKKGFEL
                     NESDFGEGKIQYLILDPDGHPWILS"
     gene            1791..2879
                     /locus_tag="PLX80_08085"
     CDS             1791..2879
                     /locus_tag="PLX80_08085"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQY20783.1"
                     /translation="MKRIKFISNNNQQKEFSTTVRKKVNDYFKENDISPKGNIALFIQ
                     TSAMLTIYLLPFILILLIPMNGWIAMLLSAIMGLGMAGVGMCVMHDALHGSYSHNEWV
                     NKILGWTMYILGSNVFNWKIQHNVLHHAYTNIEGYDHDIDSRGPLRLSENAPLHKIHK
                     YQYIHAFFLYGFITISKLVKDFSQLIEFTKLGLTKQHAHNPTMEYVKMVIFKIMYLVL
                     FVGLPIIFTEFTWYQVILGFFIMHFTGGCVLSTIFQMAHVVEGAEQLNPNVDGNIEND
                     WVVHELRTTSNFARNNLFLNWYIGGLNFQIEHHLFPNICHIHYRKISPIVEKTANEFG
                     YDYNLKPSFMNALVSHVKRLKQLGVETA"
     gene            complement(2966..5116)
                     /locus_tag="PLX80_08090"
     CDS             complement(2966..5116)
                     /locus_tag="PLX80_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQY20784.1"
                     /translation="MPSSKKSVKILKKPIKGKTVLDKKTVSKDMTDSNIKSTSLKTQT
                     KKDPAFKKASKKQTVKIRDYKLEPDKAFKIDSNIIQKEIIKKITGAPEDLEISLEPVE
                     DNFIVYQQTPGFTSLDKPLAQLSVRVVIYNKGSKPVDLDKVVFEYDQGSKAIKKKISL
                     PSDQLIIDPNYAWAWQNSRDYHKSGDVIYLEFPFPEKLTINLYFKKFSIPLIITKDLK
                     PYSVNLDIPFKSIDFSKEEYLTGYSMHGGGEQVFGYDVGVKAFEKNGWRELYWGKDGK
                     ENKHFRIWGKPVYAMADGKVLHYENNIPNNWKPDGSKEGLLKQENELWGSFDLGGSGN
                     HFYIKHGKVVALYAHLQKGSLNKKFLKKGNKIKKGDLLGKAGNSGNSTAPHFHFHIKT
                     FTKDSEPEGGIFRPLIFSNGYVIGQKNYLTPKSNINWTKLNEEGIPGLNDKACFIFPS
                     ERHPYCEYPNIWGEVCKFGIPEKNYQEEFDKIWTCGYYPVWVNAFDVKGITYFNVIFR
                     PSKNIDWVAGHNMDGSKFQNEFDKWVKEKYRLININSYLHKGNVKYAAVWIKDSSIDW
                     FAYHGKTLSWHENNFKKYSKADWVPVNVSCLHKGSKTYVSALWEKKNTGGFYLRPEMK
                     LEEFVNSFKQYTDKEKFKLVYLDGYVKNGKPRLSGIWYKNAPGYNSWWEKHHKTENQF
                     QSEYNSMLKNGYLTRCIAGYSHNSESFFEGIWSK"
     gene            5429..5899
                     /locus_tag="PLX80_08095"
     CDS             5429..5899
                     /locus_tag="PLX80_08095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY20785.1"
                     /translation="MSEINIRRAEEKDVDDLRFLYRDTVIYINSADYTSEEIKAWSDT
                     SKNTAGLKRKISEQYFIVAEIDKIITGFSSIANDGYLDFIYVHKDYQRKGVAIALLNE
                     IESQAKISDIEKIYSHVSKTARPFFEKYGYIKKGEIINKVNNIEFVNSVMVKVL"
     gene            6165..7151
                     /locus_tag="PLX80_08100"
     CDS             6165..7151
                     /locus_tag="PLX80_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20786.1"
                     /translation="MKNLFRPACLLLYLLTLIAFFFGGMTMAALTGAAKDQGLAGGAI
                     VFFYGVISIGMGFVFAMIYCFYASDKAVKTANWSLIGLIIIVAGLMAYNYNERQKEME
                     KKSELKKFNYIQNEGKFQTAGFDLNIKKNLNFKKDEFEDTQMGLGFFKPNFFEKPVIY
                     FYGQPNLEKSFQELSPTDSIVTAQAELGGFQITNAPPWLVPEHLKMDYDILYFKVISL
                     GRDLIEVEVNSTNGQTGYMDRSAGEIIFWPEFFLRINSVEFLDPVNQKVLHNNVDHAS
                     EVITLYDLMRPVLIQNEWMQVELYDDSYKVTGKGWIKWRADGKLLISYSLLS"
     gene            7416..8168
                     /locus_tag="PLX80_08105"
     CDS             7416..8168
                     /locus_tag="PLX80_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HQY20787.1"
                     /translation="MKTLLMTIFLTGIILLPVKNNFAQTETEENTKTESLPTLQSLLD
                     EKKAAFELKASDEKKKIYSDGTQAVVNSGVLENALNNGDTAPDFLLKNSAGEEVTLYS
                     YLEKGPVVLTWYRGGWCPYCNITLRRLQQELPNFQSLGASLIALTPELPDSSLSTSEK
                     QNLEFEVLSDPGNKVAGQFGIVFKLTNDVADSYQKGFDLHAYNGDESNELPLAATYVI
                     DTNKEIIYTFLDADYRNRAEPSDILDALKSSK"
     gene            complement(8236..9699)
                     /locus_tag="PLX80_08110"
     CDS             complement(8236..9699)
                     /locus_tag="PLX80_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20788.1"
                     /translation="MNILNSKLFLLIDSFSDHDIKEFKKLITSNFFSNGRNYKGLLNL
                     ILKIKKKKLKEYTSDQFYSDLFPDKKFSVQTLDNRMSELFKLAEEYLIIKTLRENKAE
                     RNKILLLAFYNQKSSRFFEKQYSRTKKLITSEPESDNKYFSLSFIDRLNISYSNEKVI
                     SENTYTNYYEHSQYITALFLKNLFDFGFEFIQQEQTNRTFDFNIVNEFLKSIEISPSI
                     INKLQSSDRSIFKIVIMEFYFYKMFKTPEEIDNYFEGRKLFVELSDSLDENYKISLYK
                     KLTNYCILRQNQGIKKFQKELFDLYNEQLKLGHFPENKKAFPSTTFRNYVLIGIIVKR
                     IKWTEDFIKKYSEYLPEDNRDDEIKLSYSKLFFSSKHYKKSLECLTSFKGQNYMHYCD
                     ASILKLCTYYETEKFEEAFSEMDKLKHYLRNHSEVPEIHREYFLNFLKIYLLLIKVKT
                     GVNNKDLTELENQINKKKLRSREHWLREKISELKNSE"
     gene            9896..11857
                     /locus_tag="PLX80_08115"
     CDS             9896..11857
                     /locus_tag="PLX80_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20789.1"
                     /translation="MKIFILTFLLMIFTMTEGMTQWTSNASDNTVISNAASDQATPKI
                     ATTSDGGCYITWFDNSVPNYSVYLQKLDRHGVPQFPNNGLLISNHTQNTFLQDFNIDV
                     DANDNAVLVFTDLRNGSQLNPFAYLISPTGNFLWGANGISLTDSTTIVQNIPVVTTTS
                     DGNFVFAWQYISGGNRIIAMQKLNSAGVKQWGSAPKKLRGTGAERWEYARLIKSNSGN
                     VIMSWIVYLGNIVTTSSTKLFTQKFDVNGNGLWTAPQDTVQNIGRIYGLSYIPTLVSD
                     GQDGAVYTWTDDRDVNTRSSVWVQRFNSTGAAQFPKNGTEATTLSTNQHYSPSTTYLP
                     STGETVTFWTETNGAQTVVGGLYGQKFDAAGNQQWGTSGKEFKAMDNNQLSFISALSN
                     DTSVVVSYSESQFASANTLVKGLKTGPSSESLWTGNIITAASFLSSKVRRQSAMDNTS
                     GMVTMVWSDNRSGSGDILAQNINLDGTLGQLTLDVTLGIEAMWNGASQVQDTVKFLIR
                     NSTSPYAAVDSFSVFLDANGNGKVYFSAPAGNYFIQTSHRNALETWSGAGIAISNVIS
                     NFNFTTSASQSFGNNSVLTLGRYCSYSGEINKDGVIDISDVSLIDNDAFNFAAGYIST
                     DVNGDNVADLTDLSVTSNNADNFVAVMKP"
     gene            complement(11963..13585)
                     /locus_tag="PLX80_08120"
     CDS             complement(11963..13585)
                     /locus_tag="PLX80_08120"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HQY20790.1"
                     /translation="MKLSKPGALNFFNVVLLAVASIQYYLLTPLNEYSETYLQTVLDF
                     DYKFKLIPGFLIFYMSIYILLLMVILFIIKSKESSDLTIFLLATIFLWSLVNFMHGYF
                     PIMNVLRPKVEGAGFFFEALNSLYKNVKPFNTLPNWHVATALLLCIAYVKNKFKRQII
                     IYIWALFIILSPLFLKMTYILDVIIAIPLPFLCYYLAEKISTVKLRTEKVQEIVKTFS
                     LESLVQSVAIGIRDESTISSLIEGLTRIEKSMNEKDKSELTNILSGFDPPLTSLKQVI
                     NNMIESISAEKQLSKAQQIYGESSKSYSPSDTEIKRVTDDMISDACKPFDNSKFRHEL
                     LELKRRNTGKINTTSIEELAKDRSNDIIFRFKSFVESHKKDIPVINKISGPADAFSEI
                     SFDDIKLFSKELRKPPYEISPDEVWNAFYRVEPDRVKPLSDLNNSVNVISLTQFVTGK
                     IEMLEPFGDIVDRKFKNWLVENESAGKKYSEEQMEWLNMMKTYVSTFLKIDMMSFNDP
                     PFINKGGAARAYNLFSTDLNKILSDLNERLVA"
     gene            complement(13673..>13979)
                     /locus_tag="PLX80_08125"
     CDS             complement(13673..>13979)
                     /locus_tag="PLX80_08125"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374495.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQY20791.1"
                     /translation="RETEKMKDGSDAIADWPILNFGLHCIGGASWVSLHHGGGVGIGN
                     SIHSGMVIVADGSKDAEVRLERCLTYDPGMGIVRHADAGYDLAIEEKDRFGINMPML"
BASE COUNT         4679 a         2272 c         2289 g         4739 t
ORIGIN      
        1 attatttaaa ttcaataaat atgaaagatg aaaaattttc aaatcaattg gaatttagca
       61 agataagaaa catgaaaata ttattaacag cgcttttatt tctgatgagt ttacctttgt
      121 ttattattaa tacatcgtgt aaaggaactc aggaaaaaat tacaaagaaa aaaaattccg
      181 aagaaaaaac ttcaaaagta aatgaaaacg attcagttat cttcagtttt gtttttgtag
      241 gatgcaatag agtagataaa agcgaacaga ataatccttc cgccactgat tcatctactg
      301 caaatttaac tgtactaaaa aagatctgtg acgaaataac taatattcag cgaaaacctg
      361 atctgttctt ttttcttgga gatcttgtat tgggagaaac taacacaaaa gaacttgaca
      421 tacagcttgg agcatgggta aaacttttag aagacactgc attcagcaag attagtaagg
      481 ctgatattga acttgtagct gttcccggaa atcatgaaat gctttacaaa aaaactcata
      541 aaaaaataaa agaccatgat gaatggccgt tgaaagatgc gacagaaatc tggatgaaac
      601 ttatgcatcc atatttacct gaagaccgtg agagaattac cggctctgac agtatcatcg
      661 aacagatgac attctctttt atcagaaata atgttgggtt tattgtaatg aatactgata
      721 catataatga gccaactgaa acggatcctt atggaattga aggaatcatt ccgacagcat
      781 ggatattgaa aaaaataaaa gaatacagag acaatcctga gatcgaacac atttttatat
      841 tgggacacaa accatattac tcttatgaca aacccaatac aggtcatgat ggtcttcccg
      901 aaggtccggt attgtggcct gaacttaata aataccacgt cattgcaatg ctttcagctc
      961 acatccatga ttatctgaga atgcaacccg gtaatgaagg gacttatcag attattgcag
     1021 gtaacggcgg tagtcaggga cctgcagagt tttttggtta ttccattatt aatattatga
     1081 aaagcggtga tgttcaattg atctcaaaag gatttgacaa aggcgatcca tattatactt
     1141 tgcctgatga tgaaccatct acagtaagag atgaatcaat tcttagctgg acaaaaaatt
     1201 ctaatccata taaaaatcct aaaaaaaaat aatgatgtgt ctataatgga aaaaagaaaa
     1261 ttaaaaaata acatcttatt tgaaaagatc gatataacca caggcaagct cgatgaaatg
     1321 gtaaagtttt atgaagatca attcggaatt gaatttgaag agatagtatt tgaaatggat
     1381 aaaaaaaaat ggaagagata tttaggaaat cttggcggta ttgtatttca tcttgttcca
     1441 aatgaaactt caaatacgga aaaagatcag acaggcgtac accaatttca tattcttgta
     1501 aaggatattg ataaatttaa aaactcagca ttcaaaaaag gatttgaatt aaatgaaagt
     1561 gactttggcg aagggaagat tcaatatctg atacttgatc ctgacggaca tccctggata
     1621 ttaagttgag tagattttat atcaaaacgg acttattcgc attttaaaat tggtacatac
     1681 taaagacttt tacaaatcaa ttgaaaagag tattttagga tctatatttt aacttcaatt
     1741 ttacaaacca atatattatt taagagtaat tcttactcat aatttcttac atgaaaagaa
     1801 taaaatttat cagcaataac aatcagcaaa aggaattctc aaccacagtc cgcaaaaaag
     1861 taaatgatta ttttaaggaa aacgatattt caccaaaagg taacatagca ttgtttattc
     1921 agacttctgc aatgctgaca atttatcttc ttccatttat actgatctta ttaattccaa
     1981 tgaatggatg gatagcaatg ttattatcag caattatggg acttggaatg gcaggagtag
     2041 gaatgtgtgt gatgcatgat gcattgcacg gatcttattc acataatgaa tgggtaaata
     2101 aaattcttgg atggacgatg tatattttag ggagcaatgt attcaactgg aaaatacagc
     2161 acaatgttct tcatcatgcc tataccaaca ttgaaggtta tgatcatgat atagattcga
     2221 gaggaccttt aagattatca gaaaatgcac cgcttcacaa aattcacaag tatcagtata
     2281 ttcatgcttt ttttctttac ggattcatta ctatttcaaa acttgttaaa gatttttcac
     2341 agttaataga gtttacaaaa ttaggattaa ctaaacagca tgcacacaat cctactatgg
     2401 aatatgttaa aatggtgatc tttaagatta tgtatttagt actctttgtc ggtcttccta
     2461 ttatctttac tgaatttaca tggtatcagg ttatattagg attttttata atgcatttca
     2521 ccggcggttg tgttctcagt actatattcc aaatggcaca tgtcgtggaa ggtgcagagc
     2581 agttgaatcc taatgtagat ggaaatattg aaaatgactg ggtagttcac gaattaagaa
     2641 cgacttctaa ttttgcaaga aataatctat tcctaaactg gtatatcggc ggactgaatt
     2701 ttcagatcga gcatcactta ttcccgaata tctgtcatat tcattacaga aagatctcac
     2761 cgatagtaga aaaaacagca aacgaatttg gctatgatta taatctcaaa ccaagtttca
     2821 tgaatgcact tgtatcacat gtcaaaagac ttaaacagct tggagtagag actgcttaaa
     2881 tagaatttat ttttatgtaa ataaaaagag gcttatataa attgtaagcc tctttttttg
     2941 ttaaaaggaa tattaataaa tcagattact tagaccagat cccttcaaag aaagattcac
     3001 tattgtgact atatcctgcg atgcatcttg taagatatcc gttttttaac atgctgttat
     3061 attctgactg aaactggttt tcagttttgt gatgcttttc ccaccatgaa ttataacccg
     3121 gtgcattctt ataccagatc ccgctgagtc gcggctttcc atttttaaca tatccgtcga
     3181 gatatacaag cttgaatttt tctttatctg tgtattgttt aaatgaattt acgaattctt
     3241 ctaatttcat ttctggcctc agataaaatc caccggtgtt tttcttttcc caaagagctg
     3301 aaacataagt cttactgcct ttatgcaggc aggacacatt taccggtacc caatctgctt
     3361 tgctgtattt tttgaaatta ttttcatgcc atgataaagt ctttccgtga tatgcaaacc
     3421 aatctatgct gctatccttt atccatactg cagcatattt cacgtttcct ttatgtaaat
     3481 aagaatttat atttataagc ctgtattttt ctttgaccca tttatcgaat tcattctgaa
     3541 atttacttcc atccatgtta tgtcccgcta cccagtcaat attttttgat ggacggaata
     3601 ttacattaaa atatgtaatt ccctttacat caaaagcatt cacccataca ggataataac
     3661 cgcatgtcca gatcttatca aattcctcct gataattttt ttcaggaatt ccaaacttgc
     3721 agacttcgcc ccatatgttt ggatattcac aataaggatg tctttcggaa ggaaaaataa
     3781 aacatgcttt atcattcaga ccgggaattc cttcttcatt taattttgtc cagttaatat
     3841 ttgactttgg agtaagatag tttttttgac caattacata accgttgctg aatatcagcg
     3901 gtctgaatat tccaccctca ggttctgaat cctttgtgaa tgttttaata tgaaaatgaa
     3961 aatgaggtgc agttgaattg cctgaatttc ctgccttacc aagcagatca cctttcttta
     4021 ttttatttcc cttcttcaga aattttttat tcaaagatcc tttctgcaga tgtgcataaa
     4081 gtgcaaccac tttcccatgc tttatataaa aatgatttcc agatccacca agatcaaatg
     4141 agccccaaag ctcattctcc tgcttcagta atccctcttt gcttccatca ggtttccagt
     4201 tattcggaat attattttca tagtgtaaaa cctttccatc tgccattgca taaaccggct
     4261 taccccaaat cctgaaatgc ttattctctt ttccgtcctt tccccaatac aattccctcc
     4321 aaccattttt ctcaaatgct ttcacgccta catcatatcc aaatacctgt tcacctcctc
     4381 cgtgcatgga atatcctgtc agatattctt cttttgaaaa atcgattgac ttaaatggaa
     4441 tatcaagatt tacagaatat ggttttagat ctttagttat tatgagagga attgaaaact
     4501 ttttgaaata taaattaatt gtaagctttt ccggaaatgg aaattctaaa tatattacat
     4561 ctccgctttt atgataatct ctgctgttct gccacgccca tgcataattc ggatcaatta
     4621 ttagctgatc agaaggaagt gatatttttt tctttatagc ttttgatccc tgatcatact
     4681 caaatactac tttatctaga tcaaccggtt tcgaaccttt attataaatt accactctta
     4741 cagaaagctg tgcaagcggt ttatcaagac ttgtaaaacc gggtgtctgt tgatatacaa
     4801 taaaattatc ttctaccggt tcaagtgata tttctaaatc ttccggagca cctgtgattt
     4861 tttttatgat ttctttttgg attatatttg aatcgatttt gaatgctttg tcaggttcca
     4921 atttgtaatc tctgattttt acagtttgtt ttttagatgc ttttttaaat gcaggatctt
     4981 ttttggtttg agtttttaat gaagtgcttt taatatttga atctgtcatg tcctttgata
     5041 cagttttttt atctaaaacg gtcttccctt ttatgggctt tttgaggatc tttaccgatt
     5101 ttttactaga gggcataaga catgaatttc aattttaaat aatacaatta atgaacggtc
     5161 aatttggatt tgaaatcaga ttttaaaaag atcaaaactt tccattactt aataattttt
     5221 aggtctattc tgaatattga tctttaagat atgataaaaa taaactttac taaacgttaa
     5281 atcaattgga aagtactttt gcttccgaaa aaatcctttt tccttttcaa ttaaaaatta
     5341 ttctaatttt ttttactata gtaataaatt gcgagttgta caaaattttt ataattttac
     5401 attcaatccc aaaaacaaat aattaagatt gtccgagatt aatatccgcc gtgcagaaga
     5461 aaaagatgtt gatgatttac gatttttata tcgtgatacc gtaatttata ttaattctgc
     5521 tgactacact tctgaagaga taaaagcctg gtctgataca tctaaaaata cagccggact
     5581 caaaagaaag atctcagaac aatattttat tgtagctgaa atagacaaaa taattaccgg
     5641 tttcagttct attgcaaatg acggctatct tgattttata tatgttcaca aagattatca
     5701 gcgtaaaggt gtagcaatag ctttgttaaa tgaaattgaa tcacaagcaa agatatctga
     5761 cattgaaaaa atctacagtc acgtaagtaa aactgccaga ccattttttg aaaaatacgg
     5821 ctatattaaa aagggtgaga tcattaacaa agtaaataat attgaatttg taaattccgt
     5881 tatggttaaa gtcttataaa tattaaatta cttttcggaa agttaattta ataaaaactt
     5941 ctgttcctat aattcattat tttaagtata tttagtaaca gactatttaa ttcaatcaat
     6001 aaatttttaa ttattttata actgatattt caaaactttt aagaaagttt gatatcttaa
     6061 catttgaaat ctatttttaa ggattttcta aaatcagctt tggagacttt tactttctgt
     6121 aagaattcaa attcttaact aaattaatta atcaatcaaa ctttatgaaa aatctattca
     6181 gacccgcttg tcttttactt tatctgctta cattgattgc tttctttttt ggaggaatga
     6241 ctatggctgc actgactggt gctgcaaaag atcagggact tgcaggaggc gcaatagttt
     6301 tcttttatgg agtcatatcc attggaatgg gttttgtttt tgcaatgatc tattgttttt
     6361 atgcatctga taaagcagta aaaacagcta actggtcact aattggatta ataataattg
     6421 ttgccggatt aatggcttat aattataatg agcgtcaaaa agaaatggaa aagaaatctg
     6481 agctgaaaaa attcaattat atacaaaatg aaggaaaatt tcagacagca ggttttgatc
     6541 taaacataaa gaaaaacctt aattttaaaa aagatgaatt tgaagatact caaatgggac
     6601 ttggattttt taaaccaaat ttttttgaaa agcctgtgat ctatttttat ggtcagccga
     6661 atcttgaaaa atcttttcag gaactttccc cgactgacag cattgttact gctcaggcgg
     6721 aattgggcgg ttttcaaatt acgaatgcac ctccttggct tgttccggaa catttaaaaa
     6781 tggattatga tattttatat tttaaagtta tcagtcttgg aagagatctt attgaagtgg
     6841 aagtcaattc tacaaatggt cagacaggct acatggatcg cagtgcaggt gaaattattt
     6901 tctggcccga attttttctt agaataaatt cagtagaatt tttagatccg gtaaatcaga
     6961 aagttcttca caataatgtt gatcatgcaa gtgaagttat tacactttat gatttgatga
     7021 gaccggtatt gatacagaat gaatggatgc aggtagagct ttatgatgac agttataaag
     7081 ttacaggtaa aggatggata aagtggagag ctgatggaaa attacttatc agctattctt
     7141 tgctaagctg agatagtttt aaagatatgt ctggaactct gcatctcact tgtctttccc
     7201 actatcatta ctacagtcat tcccggcgtg tttttagcgg gaattcaata agtaatgata
     7261 aggtttgatt tctgaaattg aagaacagac tatcagaaaa cattgaatct gcctcaaagt
     7321 atcggagact ctaagctaag aacattaaaa ttaaaatcct ttaagtcttt gaggctttga
     7381 ggtaaaagaa aattttttta atattaaata ataaaatgaa aacattactc atgacaatat
     7441 ttctaacagg tataatttta ttgccggtta aaaataattt cgcacaaaca gaaacagaag
     7501 aaaatacaaa gacagaatcg ttaccaacat tacaaagttt acttgatgaa aaaaaagcag
     7561 cttttgaatt aaaggcaagt gatgaaaaga aaaagatata ttcagatgga acccaggcag
     7621 tagtaaatag tggcgttctg gaaaatgcac taaataatgg agatactgct cctgactttt
     7681 tattgaaaaa ttctgcaggt gaagaagtta ctctttattc atatctggaa aaaggaccgg
     7741 ttgtccttac atggtacaga ggcggatggt gtccttactg caatattact ctgagaagac
     7801 tacagcagga gcttcctaat tttcagtctc tcggtgctag tttgatcgca ttgactcccg
     7861 aacttcccga cagttctctc tctacttccg aaaaacagaa tcttgaattt gaagtattaa
     7921 gtgatcccgg aaacaaagtt gccggacaat tcggaattgt tttcaagctg acaaatgatg
     7981 tggcagattc atatcagaaa ggttttgacc ttcacgcata caacggagat gaaagtaatg
     8041 aactgcctct tgctgcaact tatgtgattg atacaaacaa agaaataatc tacacatttc
     8101 tcgatgcaga ttacaggaac agggcagagc cgtcggatat actggatgcg ctaaagtcta
     8161 gcaaataaaa aaaaaattca tataaaattt agccgtcagg aattactgcc tgatggctta
     8221 tctaaaatag aatatctatt cagaattctt cagctctgaa atcttctccc taagccaatg
     8281 ctccctcgat cttagcttct ttttatttat ctgattttca agctcggtca gatctttatt
     8341 gtttacaccg gtttttactt taatgagcag aagatatatt ttcagaaaat taagaaaata
     8401 ttccctgtga atttcaggta cttcagaatg gtttctcaga taatgtttta gcttatccat
     8461 ttctgaaaat gcctcttcaa atttttctgt ttcataataa gtacacaatt ttaagattga
     8521 agcatcacaa taatgcatat aattctgtcc tttaaaactt gtcagacatt caagggattt
     8581 tttataatgc ttactgctga aaaaaagttt tgagtatgat aattttattt catcatctct
     8641 gttatcttcc ggcagatatt ccgaatattt ttttataaaa tcttctgtcc attttatcct
     8701 ttttacgata atgccgatga gtacataatt tctgaaagta gtagaaggaa atgctttttt
     8761 attttctgga aaatgaccaa gcttaagctg ttcattgtat agatcaaaca attctttctg
     8821 aaatttttta ataccctgat tctgccttaa tatacaatag tttgtcagct ttttgtatag
     8881 tgagatctta taattttcat ctaaagaatc tgagagttcg acaaataatt ttcgaccttc
     8941 aaaataatta tctatctcct caggtgtttt gaacatttta taaaaataaa attccattat
     9001 aacaatttta aatatactcc tgtcactact ttgtaattta tttattattg acgggcttat
     9061 ttcaatagat tttagaaatt catttacaat gttaaaatca aaagttctgt tagtctgctc
     9121 ctgttgaatg aattcaaatc caaagtcaaa taaatttttt aaaaataacg ccgtaatata
     9181 ttgtgaatgt tcataataat tagtgtaagt attctctgaa attaccttct catttgaata
     9241 agaaatattt aatctgtcta taaatgataa actaaaatat ttattgtcac tttcaggctc
     9301 agaggtaata agtttttttg tccgagaata ttgcttttca aaaaatctac tgctcttttg
     9361 attataaaat gctagtaaaa gaattttgtt tctttcagct ttattttccc ttaatgtttt
     9421 aatgatcaga tattcctctg caagcttaaa taattctgac atcctgttgt caagtgtctg
     9481 aacactgaat tttttatcag ggaaaagatc tgaataaaac tgatcggatg tatattcctt
     9541 caattttttt ttcttgattt ttaaaatcag atttaacaga cctttataat ttctgccatt
     9601 tgaaaaaaag tttgaagtga taagtttttt aaattccttt atatcatgat ctgaaaaaga
     9661 atcaataagt aaaaacagct ttgaatttaa tatattcatg ggtaaaaata tttagattat
     9721 atgtataaat agctttaaat taaatgaaat attaatgtat tttgaaaaat tttatgggta
     9781 gaacataact tttctttctt tgaacaattt actgattatt aataagtttg tatacatttt
     9841 taacaaagcg atattttttt taaattaaac aacataataa accaatttta taattatgaa
     9901 aattttcata cttacatttc ttttgatgat ctttacgatg acagaaggaa tgacgcaatg
     9961 gacttcaaat gcatctgaca atacagtgat cagcaatgca gcttcagatc aggcaacacc
    10021 taagatcgct actacttcag acggcggatg ttatattacc tggtttgaca acagtgttcc
    10081 aaactattct gtgtatttgc aaaagcttga cagacacgga gttccacagt ttccaaacaa
    10141 cggattgctg ataagtaatc atacacaaaa tacttttttg caggatttta atattgatgt
    10201 agatgcaaat gataatgctg tactcgtatt tacagatctg agaaacggat cacaactgaa
    10261 tccgtttgct tatctgatca gtccaaccgg aaatttcctc tggggagcta acggaatttc
    10321 acttacagat tctactacta tagttcagaa tattcctgta gttactacaa cttcagatgg
    10381 taattttgta tttgcgtggc agtatatttc aggaggcaac agaattattg caatgcagaa
    10441 actgaattct gcaggcgtaa agcaatgggg atctgcgccg aagaaattaa gaggaacagg
    10501 agcagaaaga tgggaatatg caagactgat aaaatccaat tctgggaatg tgataatgtc
    10561 atggatcgta tatcttggaa atattgtaac tacaagctcg acaaaacttt ttacacaaaa
    10621 atttgatgta aatggaaacg gactctggac agctccgcag gatactgttc agaatatcgg
    10681 caggatctat ggtctttctt acattcctac acttgtaagt gacggtcagg acggagcagt
    10741 ctatacatgg acagatgaca gagatgtaaa tacaagatct agtgtctggg ttcagagatt
    10801 taattctaca ggtgcagcac agtttccaaa gaacggtact gaggcaacta cactttctac
    10861 aaatcaacat tacagtccat cgactactta cctgccttct accggtgaga cagtaacttt
    10921 ctggactgaa acaaatggcg cgcaaacggt agtaggtgga ttgtacggac aaaaatttga
    10981 tgccgcagga aatcagcagt ggggaacttc aggtaaagaa tttaaagcaa tggataataa
    11041 tcagcttagt tttatatctg cactttctaa tgataccagt gtagtagttt catattctga
    11101 atcacagttt gcaagtgcaa atacacttgt aaaaggattg aaaacaggac cttcatcaga
    11161 atctttatgg accggaaata ttattactgc cgcatcattt ttaagcagta aggtcagaag
    11221 gcaatcagca atggataata ccagcggaat ggtcactatg gtttggagtg ataaccgttc
    11281 aggatcgggt gacatacttg ctcagaatat aaatctcgat gggactttag ggcaactgac
    11341 acttgatgta acattaggta ttgaagccat gtggaacggt gcttctcagg ttcaggatac
    11401 agttaagttc cttatccgaa acagtacttc accttatgca gctgtagatt ctttttcagt
    11461 ttttcttgat gcaaacggaa acggaaaagt ttatttttca gcaccggccg gaaattattt
    11521 cattcagact tctcatagaa atgcacttga gacatggtcc ggagcaggta ttgctatatc
    11581 aaatgtaatt tcaaatttca attttacaac atcagcttca cagtcttttg gaaataactc
    11641 tgtgctgaca ctgggaagat attgttcata cagcggtgaa ataaataaag acggtgtaat
    11701 tgatatttca gatgtatcat tgatagataa tgatgcattt aattttgcag cgggatatat
    11761 ttctacagat gtgaacggag ataatgttgc agatctgaca gatctgtctg ttacttcaaa
    11821 taatgctgat aattttgtcg cagttatgaa accttaaaat aaagataaat cagatcacaa
    11881 aaaaatgtca tcagatagat aactctgatg acattttttt tatttttaga taagattatt
    11941 ataaaacgat taaatgaaat tatcaggcaa caagtctttc attaagatca gaaagtattt
    12001 tattaagatc agtactaaaa agattataag cccgtgcagc accgccttta ttaataaacg
    12061 gcggatcatt aaaactcatc atgtcaattt taagaaaagt agatacatat gttttcatca
    12121 tattaagcca ttccatttgc tcctctgaat atttcttacc ggcagattca ttttcgacca
    12181 gccagttttt aaattttctg tcgacaatat caccaaaggg ttccagcatt tcaattttac
    12241 ctgttacaaa ttgagtaagt gaaattacat taactgaatt attcagatcg gatagcggct
    12301 ttacgcggtc aggttcgact ctgtaaaaag cattccagac ttcatcgggc gaaatttcat
    12361 aaggcggctt tcttaattct tttgaaaata attttatgtc gtcaaaagaa atttccgaaa
    12421 acgcatcagc tggtcccgag atcttattta taacgggtat atctttttta tgtgattcta
    12481 caaaactttt aaatctgaaa ataatatcat ttgatctgtc ttttgcaagc tcttcaattg
    12541 aggtagtatt gatcttccct gtattccttc tttttaattc gagaagttca tgtctgaatt
    12601 tagaattgtc aaacggttta catgcatctg agatcatatc atctgttaca cgttttattt
    12661 cagtatctga aggagaataa gatttgctgc tttcaccgta tatttgctgg gcttttgata
    12721 attgtttttc tgcagaaatg gattcaatca tgttgttaat aacctgcttt agtgaagtaa
    12781 gcggaggatc aaatcctgaa agaatattag ttaattcaga tttatctttt tcgttcatgc
    12841 ttttttcgat ccttgtcaga ccttctataa gtgaagaaat tgtagattca tctcttatac
    12901 caattgcaac tgactgtact aaagattcca gcgaaaatgt ttttacaatt tcctgaactt
    12961 tctcagttcg gagttttact gtagagattt tttcagcaag ataataacac aggaaaggaa
    13021 gaggtatcgc aattatgaca tccaagatat aagtcatctt gagaaacagt ggtgataata
    13081 ttataaaaag tgcccagata taaataataa tttgccgttt aaatttattc tttacataag
    13141 caatacacag aagcagtgca gtggctacat gccaatttgg aagtgtgtta aacggtttta
    13201 catttttata aagactattt aatgcttcaa agaaaaaacc agctccttct acttttggtc
    13261 ttagtacatt cattatcggg aaatatccat gcataaaatt aacaagactc cataaaaata
    13321 ttgtagccag cagaaatatt gtaagatcgc tgctttcttt actctttatg ataaataaaa
    13381 ttaccattaa gagtaaaata taaatcgaca tataaaatat caggaatccg ggaatgagtt
    13441 taaatttata atcaaaatcc agcacagttt gtaaataagt ttcgctgtat tcattaaggg
    13501 gagtaagcag ataatattga atagatgcaa ctgcaagcaa aacaacatta aaaaaattca
    13561 gtgcacccgg tttagatagt ttcattaatt attttccagt cttgttaaaa tttttcgtat
    13621 taaattaatt attatagtac atcatttcaa gtttgaatct ttgtatttac atttaaagca
    13681 tcggcatatt gattccaaac ctgtcttttt cttcgattgc aagatcgtat ccggcatcag
    13741 catgtctcac aattcccata cccggatcat aagtgagaca tctttccaga cgtacttcgg
    13801 catctttact tccgtctgca acgatcacca ttccggaatg aattgaattg ccaattccca
    13861 caccacctcc gtgatgaagt gaaacccagc ttgcacctcc aatgcaatga agtccaaaat
    13921 tcaggatcgg ccagtcagca attgcatcag aaccgtcttt cattttttct gtttctcgg
//
LOCUS       DAORVQ010000083        13811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2052_length_13811_cov_6.237860, whole genome shotgun sequence.
ACCESSION   DAORVQ010000083 DAORVQ010000000
VERSION     DAORVQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13811
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_13811_cov_6.237860"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="PLX80_08130"
     CDS             <1..2223
                     /locus_tag="PLX80_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20792.1"
                     /translation="NGVEVPSTEADRKSFSFDLFPSKLLENIIVAKSFTPDMPGNFSG
                     GLVQLNTKDFVDELIMNFETTGSYLEGTTSNGDFYSYNAGQSKILFFNSGKDNGGRNI
                     PSNFPSGKFSGQNNYGKTLTNNWDQENRKAPVNGGFQLALGNNFNVADNPLGVLFSYT
                     YKNGFENTSIQRNEYNSDTTTLIDYDGRISQYTVLNGGLFNANYRIGNNNKINFKNTY
                     SVNSEDNTQYYEGIYKVIADYDRKIYATDFIERSLFSSLLSGSHYLNNLNNLNITWIG
                     SYAESERNEPDTKTTFYQRELGTEDPYFAPLSTIPTANVGQRFYSFLKDIKRNLAVDF
                     DMKFLKLGNGSTSKIKFGTFATGTDRNFSARNFAPANAGSFQIGFQPIETIFSEENID
                     STKLYYVEITDKSDAYTASENLYAGFLMFDIPVNKLRIITGLRYEYNEQKLNGFVRQT
                     GDPVLVNQRNNDYLPSINLTYALNDKTNLRASATQTVSRPELREIAPFSFIDFVTEGQ
                     LTGNPELEESLIQNYDLRYELFPAAGEIVSFSLFYKHFDQPIEKIIVPTLQTAIPSYT
                     FGNAKEGAINYGMELEVRKNLGFISKSLIDLTLNANFALINSQVNLEGLQTAVSETTR
                     RLQGQSPYTINVGLFYDNYEIGFSANLLYNKFGAKISEVGRSGFNDVMEQGRDLLDLN
                     ISKTFLGNFEGKFGIRNILAQDQIFTQKFTLNENQDFEKDVRIYTTDTNFNFTLSYKY
                     "
     gene            2258..3922
                     /locus_tag="PLX80_08135"
     CDS             2258..3922
                     /locus_tag="PLX80_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20793.1"
                     /translation="MKTLLTLIVILLLFSTANVYSQVDSTTLNSSSITSNITLSRSIT
                     YLMKGFNYVQSGATITIEAGTKIFGDFNTTGTLIIQRGGKIYANGQVNDPILFTSNKP
                     AGQRKAGDWGGIIILGRSGINTSTGVDSAEIEGFGAGLGPIYGGQPRIDNDSSGLLRY
                     VRIEFPGVNLTGVSGNEINGLTMGGVGSKTVIDYVQVSYSGDDSFEWFGGTVNCKHLI
                     SYKALDDDWDCDNGFRGKVQFGLSVRDSSYADVSSSNGFEIDNNNNSPSNFNSPRTQP
                     IFSNMTVIGPYVTTSAVVNPLFQRGGHLRRNNLASIYNSIIMSWRVGLRFDGSGVGNA
                     AQADSIQIRNNIMAGNVKLADSTGTGSFSPQTWLQTSSFENSVYSDNSSVMLNSPFNI
                     YPDAPLPGNNVNNWMPDNGSPALSGSSFNNPNLNGFENVTFRGAFGSVNWSLEWAQFN
                     PREYNIIGISQISSVIPERFTLDQNYPNPFNPSTTINFSLPNSGLVSLKVYDISGKEI
                     ANLVNENLTVGTYEYKFNAASLSSGIYFYTLKSDNITETKKMLLVK"
     gene            4063..4782
                     /locus_tag="PLX80_08140"
     CDS             4063..4782
                     /locus_tag="PLX80_08140"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQY20794.1"
                     /translation="MKMIINTLIVSDIHLGSYVARPSQVLDLLKSLSFKRLILLGDIF
                     DDLNFKRLKREHWDLLSYIRKLSNPDRNIEVIWVEGNHDEGLIEKLSHLIGIKAYMEY
                     EFIINGNKIIAIHGHQFDRFLNENILISNISSFIYNKIQKYDREKLLVSRFIKRKSKG
                     WLRLSHKVADNAIEYARRKNKNTVICGHTHQILQKSQNGISYFNSGCWTDIPSSYITI
                     DNEGIVRISEFFEIEKLKLVS"
     gene            5023..6936
                     /locus_tag="PLX80_08145"
     CDS             5023..6936
                     /locus_tag="PLX80_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HQY20795.1"
                     /translation="MQRVLRIVTIIILIFGLKDSFAVTDSTTLNSSNITGLVTLNRTT
                     TYKMKGFNNVVNGGKLVIPEGTVIKGDFNSKGTLIIQRGGIIYANGTPDNPIVFTSER
                     PAGQRNTGDWGGVIILGRSAINTSTGVDSAEIEGFGPGLGPIYGGQPVVNNDSSGCFR
                     YVRLEFPGVNLTGVSGNEINGLTMGGVGSRTVIEYVQVSYCGDDSFEWFGGNVNCKNL
                     ISYKALDDDWDCDNGFRGRVQFGLSVRDKDIADVSSSNGFEIDNNNNSPSNTNGPRTR
                     PFFSNMTVIGPFENNSTSVNPLFQRGGHLRRNMQACIYNSLIMGWRTGIRFDGSGVCN
                     DANGGTVQLRTNIFSGMFTAIADQTGGTVTPDPVSFINTWNTSYTNNSDVMLTSPFNI
                     YPDPSGNSVNNWLPAGGSPALSGASFTNPNLTDFTSVAYRGAFGSINWSAVWAQFDPK
                     NYFLPVPSDFNLTVIPQAFLNTNTNMLNSSDTVRLYLRQTSAPFNIVDSSKAVVNAGS
                     FAGSFVFNNATSGTYYLQVKHRNSIETWSKSGGEPFTQGGNNSFNFTTSAAQSFGSNQ
                     ILRAGKYCMYSGDVDQDGLVELNDASLTDNDISSFTAGYVSSDVNGDGIVDLDDISFV
                     DNNTALFISVIRP"
     gene            6956..7891
                     /locus_tag="PLX80_08150"
     CDS             6956..7891
                     /locus_tag="PLX80_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20796.1"
                     /translation="MNAGREVYSRLVFLFYNFSINQNKSNMKYLAIFFLVFLSFFNSK
                     NVSAQNPEYTLTAKNFNFQAADSLTFEVFLIHTNTAVIDTFQYASGQYFFLFNPAIAN
                     GGTLSYRIIGSGLPVSQQPRNPTVSNNELRLAGNAIVGQGNGFIIPSNSPGVLVVKMS
                     LKTSAGSFAPNENLDLQWKNAPGPGFFTRIYAYVNMLNTEVTVPGNHFIDTMQVSVNQ
                     ISSLVPTKYELQQNYPNPFNPSTTINFSLPKTGLVSLKVYDISGKEISNLVNENLTVG
                     TYEYKFNAASLSSGIYFYTLKADGISQTKRMMLIK"
     gene            8306..9127
                     /gene="fdhD"
                     /locus_tag="PLX80_08155"
     CDS             8306..9127
                     /gene="fdhD"
                     /locus_tag="PLX80_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQY20797.1"
                     /translation="MISSKIIKIKKYSASDCKTKDDHVAVEEPLEISIEYGINNERVQ
                     KIISVTMRTPGNDEELAAGFLFTEGILKNSDEIFNSDNSQLKENKIVIRLNENVTPDI
                     NNAERNFYTTSSCGVCGKTSIDSVKTVSAFIDVSDNLKITADILYCLSDELKNQQKIF
                     QVTGGLHASALFDINGNFENLREDVGRHNALDKLIGNYFLENMLPLTDKILFLSGRAS
                     FELVQKAMMAGIKLVVSVGAPSSLAVSLAIESGITLTGFLKKESFNVYANDHRIQ"
     gene            9167..11482
                     /locus_tag="PLX80_08160"
     CDS             9167..11482
                     /locus_tag="PLX80_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HQY20798.1"
                     /translation="MGKEKKNIIPDHQNPESHTGLKKGKLNKSAAGIGGVISAGKKII
                     QEAGVIRGLKALNSLNKYDGFDCPSCAWPDPDDERSKLGEYCENGARAVADEATTKKL
                     TGEFFKKHSVKYLSGLSDFEIGKKGRIAEPLFLLKGDTHYKSVTWDFAFEKITEYLNK
                     LDSPDEAVFYTSGRTSNEAAFLYQLFVREFGTNNLPDCSNLCHESSGVALGESLGTGK
                     GSATLDDLYNAEVILILGQNPGTNHPRMLTALEKAKANGAKIISINPLPETGLMVFDN
                     PKSLKGLMGITSQLTDLFLQIKINSDMALLQVIEYFLLLEEEKFPGKVFDQKFIDENT
                     SGFETFSEHIKSLNVDDLINSTGVEKIEILKAVDLLKNKSKIIACWAMGLTQHKNAVY
                     TIKEIVNLLLLKGSIGKKGTGTFPVRGHSNVQGDRTMGINENPSHEFLDKLDEVFSIN
                     SPRKKGYDVVESIKAMFEGKAKVFFAMGGNFLSASPDTEMTAQALRNCRLTVHVSTKL
                     NRSHLIHGEEALILPCLSRSDKDLLNGKEQFVSCENSTGIVQSSKGVLVPVSDKLLSE
                     PVIVCNLAKATFKKSGVKSKINWDRYMKDYDEIRNDIEKIIPGFSDYNKRVREPGGFY
                     LPNSNREGKFDTANSKANFNISNFIPIELKEDELLLMTIRSHDQFNTTIYGLDDRYRG
                     IYNERRVIMMNKKDIENRRLINGDKVDLYNFSGNKERKAGNFIVTEYPIPEGNAAAYF
                     PETNVLVPIESVADKSNTPASKQIVIKIKKR"
     gene            complement(11501..13411)
                     /locus_tag="PLX80_08165"
     CDS             complement(11501..13411)
                     /locus_tag="PLX80_08165"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY20799.1"
                     /translation="MRFLISLICVVFLYSGSLYSQCMNVYNCWENQFQGAGNGKDIFY
                     DIEVSKTTNNVYTTGETFTAGGANADYITIKYNPDGVILWTRIYDGPGSGNDIAYSIA
                     IDDSDNVYITGESKGAGSEGQDYATIKYNSNGDLKWVNRYSGPSGNADQAYSIVLDAS
                     RNVYVTGNSHQGGSTNQDFTTIKYNSNGTQLWIKINDYGAADFAKKVILDNSKNVYVT
                     GYNAGANGTAHDFRTTKYDSNGVVKWSRSFNYPANSHDEPNDIAVDNLGNVYVTGFSV
                     GFFGNQYAIIKYNSAGDSVWHNNYLYVGALHEAKAILLDSVGNVYITGSSSNGSNMDI
                     ATVKYNSVTGVQMWAKRYNGTGNGRDEGTSIVQGKNGKIFVGGGSKGNLTGDDFVIIE
                     YDMTTGDSCFVSRIAAPTNETINAMTIDYDEGYIFACGSTGEGTGVTNAVSARYCLLG
                     VVNSYFNITASLEGLYDSGTNKLRMKDNIKGYLRRTVSPYLLEDSSNTVLDSATLQMT
                     CVFNNSPTGNYFIVIKHRNSIETWSRIGGEPFVCGTEMNYTFSNSLSKAYGGNMKLVD
                     TSPNKYAFYTADVNQDGIVDITDGSLIDNDAFNFASGYIPTELNGDGFVDIDDALYAD
                     NNGFNFVSLVRP"
BASE COUNT         4683 a         2235 c         2515 g         4378 t
ORIGIN      
        1 aacggtgtcg aagtgccgag tacagaagct gacagaaaat cattctcatt tgatctcttc
       61 ccttcaaagc ttcttgaaaa tattattgta gcaaaatcat tcactcctga catgccgggt
      121 aatttctcag gcggactggt acaacttaat acaaaggact ttgtagatga actgatcatg
      181 aattttgaaa ctacaggttc atatctggag ggtactactt caaacggaga tttttattct
      241 tataatgcag gtcagagtaa aatacttttc tttaattcag gaaaagataa tggcggcaga
      301 aacattcctt ctaacttccc ttcaggaaaa ttctcaggac agaataacta cggaaagact
      361 ttaactaata actgggatca ggaaaacagg aaagctcctg tcaacggcgg tttccagctt
      421 gctctcggaa ataattttaa tgtagctgac aatcctctcg gagttttatt ttcatatact
      481 tataaaaacg gatttgagaa tacgtcaatt cagagaaatg aatacaactc agatactacc
      541 actcttatag attatgacgg aagaatttcg cagtatacag ttctgaacgg tggtttattt
      601 aacgcaaatt acaggatcgg taataataat aagatcaatt ttaaaaatac atactcagta
      661 aattccgaag acaacacaca atattatgaa ggcatttata aagtaatagc tgactatgac
      721 agaaaaatat acgctactga ttttattgaa agaagtcttt tctcttcact tctgtcggga
      781 agtcactacc ttaacaattt aaataatctg aatataacat ggattggttc ttatgcagag
      841 tctgaaagga atgagcctga tacaaaaact actttctatc agagagagct tggtactgaa
      901 gatccttatt ttgcaccgct ttctactatc ccaactgcaa atgtcggtca gagattttat
      961 tcatttctga aagatataaa aagaaattta gctgtagatt ttgatatgaa gtttctaaaa
     1021 ttaggaaatg gaagtacttc aaaaataaag ttcgggactt ttgctacagg gacagacaga
     1081 aatttcagtg caagaaattt tgcacctgca aatgccggtt catttcagat aggctttcag
     1141 ccgatcgaaa ctatcttcag tgaagagaat attgattcga caaaacttta ttatgtagag
     1201 atcactgaca agagtgatgc ctacacagct tcggaaaatc tttatgcagg ctttttaatg
     1261 tttgacatac ctgtaaataa attaagaatt attaccggac tgcggtatga atataatgaa
     1321 caaaagctga atggatttgt cagacagaca ggagatcctg ttttagtgaa tcagagaaat
     1381 aatgattatc tgccttcaat aaatctgacc tacgctttga atgacaaaac caatttacgg
     1441 gcatcagcta ctcagacagt ctcaagaccc gagcttagag agattgcccc gttctcattt
     1501 attgattttg ttactgaagg tcagcttacc ggtaatcctg aacttgaaga aagtctgatt
     1561 cagaattatg atcttcgata tgaattattt cctgcagcag gtgagatagt ttcattcagt
     1621 ttattttata agcatttcga tcagccgata gaaaaaatta ttgtaccgac tctgcagact
     1681 gcaattccct cttatacttt tggaaatgca aaagaaggag caataaacta tggaatggaa
     1741 ctggaagtca gaaaaaattt aggatttatt tcaaagtctt tgatcgacct tacattaaat
     1801 gcaaattttg ctctgataaa ttcacaggta aatcttgaag gtcttcagac tgcagtttcg
     1861 gaaactacga gacgattgca gggacagtca ccttatacta ttaacgtcgg actattttat
     1921 gataactatg aaattggctt cagcgcaaat ttgttatata acaaattcgg cgcaaagatc
     1981 tcagaagtag gaagaagtgg ttttaatgat gtcatggagc agggaagaga tcttcttgat
     2041 cttaatattt caaagacatt ccttggaaat ttcgaaggta aatttggcat cagaaacata
     2101 cttgctcagg atcagatctt cacacaaaag tttactttaa atgaaaatca ggattttgaa
     2161 aaagatgtca ggatctatac tacagataca aatttcaact ttacactttc ttataaatat
     2221 tgatttaaaa atctattaac taaaaataat aataaaaatg aaaacattat taactttaat
     2281 tgtaatactg ttgctattta gcacggctaa tgtttattca caggtggact cgaccacatt
     2341 aaattcgagc agcatcacat cgaatataac tctatccaga tctataactt atctgatgaa
     2401 aggatttaat tatgtacaaa gcggtgctac tataaccatt gaagccggta caaaaatttt
     2461 cggtgacttt aatactacag gaactttgat tatacaaagg ggcggaaaga tctatgcaaa
     2521 cggacaggta aatgatccaa tcttatttac cagcaacaaa cctgcaggtc agagaaaagc
     2581 aggtgactgg ggaggtataa ttatactggg aagatccgga atcaatactt cgacaggtgt
     2641 tgactctgca gagatagaag gatttggtgc aggtctcgga ccgatctatg gaggacagcc
     2701 gaggatcgat aatgacagtt cgggtttatt aagatatgtt aggatagaat ttccgggagt
     2761 gaatcttaca ggagtttcag gaaatgaaat caacggactc acaatgggcg gagtcggaag
     2821 taaaacagta attgattatg tacaggtaag ttacagcgga gatgattctt ttgaatggtt
     2881 tggaggtact gtaaattgta aacatttgat ttcttacaaa gcattagatg acgactggga
     2941 ttgtgataat ggattcagag ggaaagttca gtttggatta agtgtaagag acagttcgta
     3001 tgcagacgtg agttcatcta acggatttga aattgacaac aataataatt ctccatctaa
     3061 ttttaattca ccaaggacac agcctatatt ttcaaacatg acagtgatcg gaccttatgt
     3121 gactacttca gctgttgtca atccgttatt tcagaggggt ggacatttga gaagaaataa
     3181 tcttgcgagt atttataact ccattataat gtcatggaga gtcggtttga gatttgacgg
     3241 atcaggagtt ggaaacgctg cacaggcaga ttcgatacaa ataagaaata atataatggc
     3301 aggtaatgta aagcttgcgg acagcacagg tactggttct ttttctccac agacatggct
     3361 tcaaacatcg tctttcgaaa attctgttta ttccgataat agttctgtaa tgttaaattc
     3421 tccttttaac atttatcccg atgcgccgtt gccggggaat aatgtaaata actggatgcc
     3481 tgacaacggc tctccggcac tttcgggttc ctcatttaat aatccaaatc taaacggatt
     3541 tgaaaatgta actttcagag gcgcgtttgg atctgtaaac tggtctctcg aatgggctca
     3601 gttcaatcca cgagaatata atatcattgg aattagtcag atctcttctg taatacctga
     3661 gagatttaca cttgatcaaa attatccaaa cccatttaat ccttcgacta ctattaattt
     3721 ttcacttcca aattcagggc ttgttagtct taaagtatat gatatttcgg gtaaggaaat
     3781 tgcaaatctt gtaaatgaaa atcttactgt cggtacctat gaatataaat tcaatgcagc
     3841 ttcactttcg tcgggaattt atttctatac tttgaaatct gataatataa ctgagactaa
     3901 aaagatgctt cttgtaaaat aaatttttga aataattaat aaatgtaaag accggggtca
     3961 attatgatca cggtcttttt tatttgcaaa ttaacaatat catttatatt tgttattaat
     4021 tgcaaaacat cagaaaagta attttaaaaa aaaattatat ttatgaaaat gatcattaac
     4081 actttaattg tttcagatat acatctgggt tcttacgttg ccagaccgtc tcaggtgctg
     4141 gatctgctga aaagtttaag ttttaaaagg ctgatcctgc tcggagatat atttgatgat
     4201 ctcaatttta aaagattaaa aagagaacac tgggatctgc tttcatacat aagaaaactt
     4261 tcaaaccccg acaggaatat tgaagtgatc tgggtggaag gaaatcatga tgaaggtctg
     4321 atagaaaaac tttctcatct gataggaata aaggcatata tggaatatga atttataatt
     4381 aacggaaata aaataattgc aatacacggt catcagtttg acagattttt aaatgaaaat
     4441 attcttatca gtaatatctc ttcattcatt tataacaaaa ttcaaaagta tgacagagaa
     4501 aagctgcttg tttcaagatt tataaaaagg aaaagcaaag gatggctcag actctctcac
     4561 aaagtcgctg acaacgcaat tgaatatgca cgcagaaaaa ataaaaacac tgtgatctgc
     4621 ggacatacac atcagatact tcagaaatcg caaaatggaa tctcttattt taattcgggc
     4681 tgttggacag acattccttc gagctatatt acaattgata atgaaggaat agtgagaata
     4741 agtgaatttt ttgaaattga aaaactgaaa cttgtttcgt gaaaattaat cctgttatta
     4801 taacatcacg atctattgat cgaattattt caagagtaat tgagattttt ctcaaactca
     4861 ctaaactgca ttttcaaaat atatcaaaaa actttcctta tttacaattt agtattctca
     4921 tcttaacata cataacattt cagtaatcct ttagaaatag ttaaatgtta tttttgtaat
     4981 acataaaaca aataatttat aactaaaaaa aaggagatag aaatgcaacg agttttaaga
     5041 attgttacaa tcataatttt aattttcgga ttaaaagact ctttcgcagt tacagattct
     5101 actacattaa attcatctaa tattaccggc cttgttactt tgaacaggac tactacatac
     5161 aaaatgaaag ggttcaacaa tgtagtaaac ggcggcaagc ttgtaattcc tgaaggtaca
     5221 gtaattaaag gagattttaa ttcaaaagga acattgataa ttcagagagg cggaattatt
     5281 tatgcaaatg gaacacctga taatccaatt gtctttacaa gtgaaagacc tgcaggtcag
     5341 agaaataccg gtgactgggg cggagtgatt atcctcggaa gatctgcgat taacacatcg
     5401 acaggtgtgg attctgcaga gatagaagga ttcggacccg gacttggacc aatctatggt
     5461 ggtcagcctg tagtgaataa tgacagctcc ggatgtttca gatatgtaag gcttgaattc
     5521 ccaggtgtaa atcttacggg agtttcaggt aatgagatca atggacttac aatgggaggt
     5581 gttggaagca ggacagtaat agaatatgtt caggtcagct actgcggtga tgattctttt
     5641 gaatggtttg gcggaaatgt aaattgtaaa aatctgatat catataaagc attggatgac
     5701 gattgggatt gtgataatgg attcagagga agagtccagt ttggattaag tgtaagagat
     5761 aaagatattg ctgatgtcag ttcatccaat ggatttgaaa ttgataataa taacaattct
     5821 ccttcaaata caaacggtcc gagaaccaga cctttctttt ccaacatgac agtgatcgga
     5881 ccttttgaaa ataattcgac atcagtaaat ccattatttc agagaggcgg tcatctgaga
     5941 agaaatatgc aggcctgcat ttataattca ttaattatgg gatggagaac cggaatcaga
     6001 tttgacggat ccggagtatg taatgatgca aatggtggaa ctgttcagct gaggacaaat
     6061 atattttcag gaatgttcac ggcaattgca gatcagacag gaggcacagt aactcctgat
     6121 cctgtgtctt ttataaacac atggaatact tcttacacta ataattctga tgtgatgctg
     6181 acaagtccgt ttaacattta tccggatcca tcaggtaatt ctgtaaataa ctggttgcct
     6241 gctggcgggt cacctgcttt aagtggtgca agttttacca atccaaattt aacagatttt
     6301 acttctgttg cttatagagg tgcttttggt agtataaact ggtctgcagt atgggctcag
     6361 tttgatccta aaaattattt tctgccggta ccttctgatt tcaatctgac agttataccg
     6421 caggcgtttc taaatacaaa tacaaatatg ctgaacagca gtgatactgt aagactatat
     6481 ctgagacaaa cttcagctcc atttaatatt gttgattcat ccaaagcagt tgttaatgct
     6541 ggttcatttg caggatcttt cgtatttaat aatgcaacat caggaacata ttatttacag
     6601 gtaaagcaca gaaattcaat tgaaacctgg agtaaatctg gtggagagcc atttactcag
     6661 ggtggtaata attcatttaa tttcacaact tcagcagctc agtctttcgg cagtaatcag
     6721 atattaagag ccggtaaata ttgtatgtac agcggtgatg ttgatcagga tggattagtt
     6781 gaattaaatg atgcttctct cacagataat gatatttcca gttttactgc gggttatgtg
     6841 agctctgatg taaatggtga tggaattgta gatttagatg acatttcatt tgtagataat
     6901 aatacagcac ttttcatttc tgtaataaga ccctaagctg atgcttagtt aatagatgaa
     6961 tgcaggacgt gaagtatatt cacgtcttgt attcttattt tataattttt ctataaacca
     7021 aaataaatca aacatgaaat atttagcgat atttttttta gtttttcttt ctttctttaa
     7081 ttcaaaaaat gtatctgctc aaaatcctga atatactctg actgcgaaaa actttaattt
     7141 tcaggcagcc gattcactta ctttcgaagt attcttaatt catacaaaca ctgcagtaat
     7201 agatactttt caatatgctt ccggacaata ttttttcctg tttaatccgg ctattgctaa
     7261 tggcggtact ttgagttaca gaataattgg ttcgggactt cctgtaagcc agcagccaag
     7321 aaatcctact gtttcaaata acgaactgag actcgcagga aatgctattg tcggacaagg
     7381 aaatggattt ataattccat caaactcacc tggtgttctt gtcgttaaaa tgagtctgaa
     7441 aacttctgca ggatcttttg ctccaaatga aaatcttgat ctccaatgga aaaatgcacc
     7501 cggtcccgga ttttttacaa ggatctatgc ttatgtaaat atgctgaata ctgaagtaac
     7561 tgttcccgga aatcatttta ttgatactat gcaagtttca gttaatcaga tatcttcttt
     7621 agttccgact aaatatgaat tacagcaaaa ttatccaaac ccatttaatc cttcgacaac
     7681 tataaatttt tcactaccaa agaccggact tgtaagtttg aaggtttatg atatttcagg
     7741 taaggaaatt tcaaatcttg taaatgaaaa tcttactgtc ggtacctatg aatataaatt
     7801 caatgcagct tcactttctt cgggtatata tttttatact ctgaaagctg atggaatttc
     7861 tcagacaaag cgtatgatgc ttataaaata aattaaaaat taggaattaa gaatttttaa
     7921 tgtgaaaatt ttcccgcaga gaaagatagt ttctatgcgg gattttttat aatacatgtc
     7981 tttgcgaacc cgaattcatt cgggtgaagc aatctgaatt ttatacttat ctatatttaa
     8041 aagaatacag attcctaaat aaaatgagaa atcaccgctg gaaaatttga cataatattt
     8101 atagattgcg tcgtcgctcc gctccccgga aaatcatttt tcaagtattt tctctgagaa
     8161 taaatgtgaa aattctttag aggttatatt attttaaaat agaataatat gaattagtta
     8221 tttaatcttg aaatatgaat tcttatttta ctctgtgtta ataattaaat tttaatttaa
     8281 aacttcaaaa aagtttatca ctcaattgat ctcatccaaa atcattaaaa taaaaaagta
     8341 ttcagcatcg gattgtaaaa caaaagatga tcacgtagcc gttgaagagc cgcttgagat
     8401 ctccatagaa tatggaataa ataatgaacg tgtacaaaaa ataatttctg taacgatgag
     8461 aactcccgga aatgatgaag agcttgctgc agggttttta tttacagaag gtattttaaa
     8521 aaactctgat gaaattttta attcagataa ttctcaatta aaagaaaata aaatagttat
     8581 cagattaaat gaaaatgtaa cccctgatat taataatgct gaaagaaatt tttatacaac
     8641 ctcaagctgc ggcgtctgcg gtaaaacaag tattgactct gtaaagactg tatcagcttt
     8701 cattgatgtt tcagataatt taaagatcac tgcagatatt ctctattgcc tctctgatga
     8761 attaaaaaat caacagaaga tctttcaagt tacagggggt cttcatgcat cagcattgtt
     8821 tgatattaat ggaaattttg aaaatctacg tgaagatgtc ggaaggcata atgctcttga
     8881 taaacttatc ggaaattact ttttagaaaa catgcttccg ttaactgata aaattttatt
     8941 tcttagcggc agagcaagtt ttgaacttgt tcagaaagct atgatggcag gaattaaact
     9001 cgtagtctct gtcggagcac cttcgagtct ggcggtttct ttggctattg aatcaggcat
     9061 tacacttaca ggttttctga aaaaggaaag ttttaatgtt tatgcaaatg atcacaggat
     9121 acaatgataa aaggactgtt aaataaatct gaataaaaat acataattgg gtaaagagaa
     9181 aaaaaatatt attcccgatc atcagaatcc cgaaagtcat accggtctga aaaaaggtaa
     9241 actcaataaa tcagcagccg gtattggtgg tgtgatatct gcaggcaaaa agatcattca
     9301 ggaggcagga gtaatccgcg gactcaaagc attgaatagt cttaataagt atgacggttt
     9361 tgactgtccg agctgtgcct ggccggatcc tgatgatgaa aggtctaagt tgggagagta
     9421 ttgtgaaaat ggtgcaagag cagttgctga tgaggctact acaaagaaac ttaccggtga
     9481 gttttttaaa aagcattctg tcaaatattt atcaggttta agtgattttg agattggaaa
     9541 aaaaggcagg attgctgaac ctttattttt attaaaaggt gatacgcatt ataaatctgt
     9601 aacatgggat tttgcttttg aaaaaataac agaatattta aacaaacttg attctcctga
     9661 tgaagctgta ttttatactt caggaaggac gagcaatgaa gcagcatttc tgtatcagct
     9721 ttttgtaaga gaattcggta cgaataatct tcccgactgc agtaatcttt gtcatgaaag
     9781 cagcggtgtt gcacttggag aatctctggg aactggtaaa ggatcggcaa ctcttgatga
     9841 tctttacaat gccgaagtca tattgatact cggtcaaaac ccgggtacaa atcatcctcg
     9901 aatgctgact gctttggaaa aagcaaaagc caacggtgcg aagataattt ccataaatcc
     9961 tctgcctgaa acaggtctga tggtttttga taatccgaag tctttgaaag gtctcatggg
    10021 aattacatca caactaactg atttatttct gcagataaag atcaacagtg acatggctct
    10081 cctgcaggtg atagaatatt ttcttttatt ggaagaagag aaatttccgg gtaaagtatt
    10141 tgatcaaaaa ttcattgatg aaaatacttc cggctttgaa acattttctg aacatatcaa
    10201 aagtctcaac gttgatgatc tgattaattc tacaggtgtt gaaaaaatag aaattctgaa
    10261 agctgttgac ctgcttaaaa ataaaagtaa gatcattgca tgctgggcaa tgggtctgac
    10321 tcagcataaa aatgcagttt atacaataaa agaaattgta aatttattat tgctgaaagg
    10381 aagtattgga aaaaagggga caggtacttt tcctgtaaga ggtcacagta atgtacaggg
    10441 cgacaggaca atgggaataa atgaaaaccc gtctcatgaa tttttagata aactcgatga
    10501 ggtattttca attaattcac cgaggaaaaa aggatacgat gtagtcgaaa gtataaaggc
    10561 aatgtttgaa ggaaaagcga aagtgttttt tgcaatggga ggaaattttt tgtcagcttc
    10621 acctgatact gaaatgactg cacaggcatt aagaaattgc agactcacag tgcatgtctc
    10681 gacaaagctt aacagaagtc atcttataca cggtgaggaa gcgctgatac tgccatgtct
    10741 cagcagaagt gataaggatt tattaaatgg aaaggaacag tttgtatcct gtgaaaattc
    10801 tactggtata gtgcagtcat caaaaggcgt actggtgccg gtgtctgata agcttttgag
    10861 tgaaccggta attgtttgta atcttgcaaa agcaacattt aaaaaatcag gagttaaaag
    10921 taaaattaac tgggacaggt atatgaaaga ttatgatgaa ataagaaatg acattgagaa
    10981 aatcattccc ggattttcag attataataa aagagtaaga gagccgggtg gattttatct
    11041 gccaaacagt aatcgtgaag gtaaatttga tactgcaaat tcaaaagcaa attttaacat
    11101 ttcaaatttt atccctatag aactaaaaga agatgaatta ttattgatga ctatcagaag
    11161 tcacgatcag tttaatacta ctatatatgg attagatgac agatacagag ggatctataa
    11221 tgagaggaga gtgataatga tgaataaaaa agatattgaa aacagaagat taatcaatgg
    11281 tgataaagta gatctatata atttttctgg aaataaagaa cgtaaagcag gaaattttat
    11341 tgtaacagaa tatcctattc ctgaaggtaa tgcagctgct tatttccctg aaacaaacgt
    11401 attggttccg atcgaaagtg tagccgataa aagcaatacg cctgcctcaa agcaaatagt
    11461 aataaaaatt aaaaaacgtt aaaacataaa tattatttca ttaaggtctg accaagctta
    11521 caaaattaaa tccgttattg tcagcatata atgcatcatc tatatctaca aacccatcac
    11581 cgtttaattc agtaggaatg taaccagaag caaaattgaa ggcatcatta tcgatcagac
    11641 ttccgtcagt aatatctact atcccatcct gatttacgtc agcagtataa aaagcatatt
    11701 tattcggaga agtatctact aacttcatat tacctccata tgcttttgat aatgaattgg
    11761 aaaatgtata attcatttca gtaccgcaga caaagggttc tccgcctatt ctgctccagg
    11821 tttctattga atttctgtgt ttaattacaa taaaataatt ccctgtgggt gaattattaa
    11881 atacacaggt catctgtaat gttgcagaat ccagcacggt atttgaggaa tcttccagta
    11941 aatatggaga aacagttctt cttaaatatc cttttatgtt atctttcatt ctgagtttgt
    12001 tagtaccgga gtcatatagt ccttcgaggg aagctgtaat attgaaatat gaatttacaa
    12061 caccaagtaa acaatatctt gcagagactg catttgttac gcctgttcct tctcctgtcg
    12121 aaccgcatgc aaaaatataa ccttcatcat aatctatggt cattgcattt attgtctcat
    12181 ttgtcggtgc agcaattcgg cttacaaaac aggaatcacc tgtggtcata tcatattcta
    12241 ttattacaaa atcatctccc gttaaatttc ctttgcttcc gcctcctaca aagatcttac
    12301 catttttacc ctgtacaatg gaagttcctt catctctgcc atttccggtt ccgttatatc
    12361 tttttgccca catttgaact ccggttacag aattatattt aactgttgct atatccatat
    12421 ttgatccatt actgctggat ccggttatat atacatttcc gacagaatca agcagtattg
    12481 cttttgcttc atgaagtgcg cctacatata aataattatt atgccataca gaatcacctg
    12541 cagaattata ttttattatt gcatattgat ttccaaaaaa tccaacacta aatcctgtaa
    12601 cataaacatt cccgagattg tcgacagcta tatcatttgg ttcatcatga ctgtttgccg
    12661 gataattaaa acttctgctc catttaacaa caccgttaga atcatacttc gtagtcctga
    12721 aatcatgagc agtcccattt gcacctgcat tgtatccggt cacataaaca ttttttgaat
    12781 tatccaggat aacttttttt gcaaaatctg cagcaccata atcatttatt ttaatccaca
    12841 attgtgtccc gtttgaatta tatttaattg ttgtgaagtc ctgatttgtg cttccgccct
    12901 gatgagaatt acctgtcaca tatacatttc tggatgcatc caaaacaata gaataagcct
    12961 gatcagcgtt gcctgaagga ccgctatatc tgttaaccca ttttaaatca ccgttagagt
    13021 tatatttaat agtagcataa tcctgtcctt cagaaccggc acctttactt tctcctgtta
    13081 tataaacgtt gtccgaatca tcaatggcaa tagaataagc aatatcgttt ccacttccgg
    13141 gtccatcata aatccttgtc cataatatta ctccgtcagg attatacttg attgtaatgt
    13201 aatcagcatt agcaccgcct gcagtaaatg tttctccggt ggtatataca ttgttggtgg
    13261 ttttacttac ttcaatatca tagaagatat cttttccgtt tccggctccc tgaaactggt
    13321 tttcccagca gttatataca ttcatacact gtgaatacaa agatcctgaa tataaaaaca
    13381 ctacacaaat tagtgaaatt aaaaatctca tttggcttta attaaatttt tgtaattggt
    13441 ttttgggtga ttggtttcat tcaaattaaa tttatatact tttaaagcta taagatgtaa
    13501 aagaataaat tcaatttcaa accttggggg agttcgaaaa aaaatttctg taaaaaattg
    13561 ttttatcttt tctttagtaa gaaagttgat ttcaaaatta tttttattgc tttgaaaatc
    13621 aagtcaaaac tcataagtgc agcaaaaata tttcccgttc cgtttctttc gtattccttc
    13681 atcaaaatgt ataaataaag gaattgaaaa atgcacttga tgttgtaatt ataaatcgta
    13741 tatttacgat atacaaatta atagttaata gttaatagtt aatagttaat agttaatagt
    13801 taatagttaa t
//
LOCUS       DAORVQ010000084        13611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2104_length_13611_cov_4.876586, whole genome shotgun sequence.
ACCESSION   DAORVQ010000084 DAORVQ010000000
VERSION     DAORVQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_13611_cov_4.876586"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PLX80_08170"
     CDS             <1..1522
                     /locus_tag="PLX80_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HQY20800.1"
                     /translation="GIYSFAILDNRDKQKVILCRDPSGVKPLYYFSEKNINIFGSEIK
                     ALLPTVKDKVSINENVVKCFLNLGYCPEPYTIYNEIKCLQPGYVIEISENEITEKVIW
                     NYEFESENNLSIKENTELTESKLRTAVERNLVADVEVAVALSGGIDSSLIYAFANQSN
                     SNIKGLTVKFDDEEYNEEDIALKYVKTLLGKHESVEVESGLDLETLNFILNDFDQPYA
                     DSSAVNVFYLTKAAGKYTKVLIGGDGGDELYNGYPSQTWLTFVEKIVSNGFMKASGTA
                     SVKIANKFSSGSNARLFKRMRDLFYDKPHELFYDWHSWFPRKTEFENGSPFLFDTNDG
                     LTLYNSVFAEKLPDKFTSKVVFDYFRKQMLSDYLRKTDMMSMLNSVEYRVPMLDEDLT
                     EFALGIPFSQKSSLRETKKILRDIHGKIYPAETSKIPKKGFMIPLDKALSSEEFEIIK
                     EELLFKGNIVLNYIKKEYVEFLFKSLKYGAENEISRAGIYQRILMLYSLSLWEKNK"
     gene            1519..2637
                     /locus_tag="PLX80_08175"
     CDS             1519..2637
                     /locus_tag="PLX80_08175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20801.1"
                     /translation="MKLAIISHTPHYNKDGKIVGWGPTVREINHLSKIFDRIYHIAPM
                     HNEQAPGSSMRYDEDKVQYIPIKQYGGEKFSEKFSILTTSFHNLKIIREVLKKVEWAQ
                     FRAPTSMGLYVLPYLSLMSKPKRWVKYAGNWGQKNPPLSYRIQRWWLKNNFQRSKVTV
                     NGKWKDEPENILDFRNPCLDNEELSRAKIISSNKNFDDKKILCFSGSLSKNKGVKLII
                     EALELMRFKDKIEEFIFTGGGHDKEMFEIAASKLKIKTTFTGFISRKELEKIYERSHI
                     IVLPSESEGFPKVISEAAAYGCVPIISDVSAVKEYFNDSNGFLLKSLNAEHLADAIDL
                     ALSNVELIKLKSRQCTELAPLFTYEDYMKNLKDKILNN"
     gene            2697..4034
                     /locus_tag="PLX80_08180"
     CDS             2697..4034
                     /locus_tag="PLX80_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY20802.1"
                     /translation="MKNSPFSAEIYAGLNVAIAMVMLFNSKLSVVYSIIILLAGVYAI
                     FKNKNMDGAAHIYAAYVVGIEVLLRMNSGGFGYEFGKYATIVFLVLGILVGKRNRKLP
                     VSFIAFILLMVPSIFFVDYPTFDMSRQMVSFNISGPVTLGIAGIYFYRRRIPIQQLRK
                     TFLAMLYPIIAMSIFVYFRSGSLEDVDFTTESNFQASGGFGPNQVSTMFGLGILIIAA
                     AYFLNIRLFRVKNLNIILLLAFLIRGLATFSRGGMLAPIVAIFLCVVIMSVTDASFQD
                     KMNRVVYIFMALFVVSVVGFYYVNEVSGGLLEMRFKGESMYDKEKSNMFSGRLEIFEE
                     DIEVFQDNVLTGVGPGMASEKRAEISGVFAAAHIEYSRLLAEHGLFGIIAILIMVLFP
                     VSTFFKLKSGDEKIMLILCTVFVFITLGHAAMRLAAPGFIYGLGFIYIFRKRS"
     gene            4031..5050
                     /locus_tag="PLX80_08185"
     CDS             4031..5050
                     /locus_tag="PLX80_08185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20803.1"
                     /translation="MILYAGNILSKFGYTPTFIETLTPKLSESYDLISVSDKKNQVLR
                     MLDMIKAVYKNRKSIKLVLIDSYSVRAFWYTYILAKLCKKFQIPYIPILRGGGYPYRL
                     KSTPEKCKFVFENSYINISPSVFLKEHFENSGYKVTYIPNFIPIENYKYKNRISTGPK
                     ILWVRSFHEIYNPLLAIEILKDLRLKYSEAKLCMIGPDKDGSLVKVEEASVENKLNGS
                     LILTGKLSKKEWTDISEDYDIFINTTNFDNHPVSVIEAMALGLPVISTNVGGIPFLIK
                     DREDGVLVPANDKEKFVSEIENLISDNKFASEISANARKKAEEFDWDQVKYKWFEVID
                     SLVKN"
     gene            5071..6378
                     /locus_tag="PLX80_08190"
     CDS             5071..6378
                     /locus_tag="PLX80_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HQY20804.1"
                     /translation="MSFLELSLKLKGFPLSKAEKEIQYLNSISETDFSKWQTDKKWEI
                     VKYHLKNNEFYRNKLENKLPAKWEEIPILKKSDFQISIDKLTSDTFNSKELYTGYTSG
                     SSGHPFRYAKDKYSHAMTWALIKNRYNSFGINLNSKQARFYGIPLEKKEYYVEKTKDL
                     LSNRVRFPVFDLSDEVLKSYIEIFRKTEFDYIYGYSNSILLFARYLNRNKINLKLICP
                     SLKVCICTSENCSTDDKVEISKAFGIPVVNEYGTSEVDLIAFEDIEGNWKLSEENVFV
                     EILDESGKQLTEGGEGRIVLTSLHNKAMPFIRYEIGDRAVIEPGKDRVRIKNLLGGIN
                     DDLVLPSGKISSSITFYFVTRSILNKIDSLKEFVIKQTEPDKFVFEMVSDEDISERDR
                     EQIRKKMDEYLEPGLIAEFKRVDKIQRTNAGKMKQFYSFVNQD"
     gene            6375..7565
                     /locus_tag="PLX80_08195"
     CDS             6375..7565
                     /locus_tag="PLX80_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20805.1"
                     /translation="MKLAILTQYFYPETGAPQNRLYELSKKFKEYGWEVMVITGMPNY
                     PKGKIFPGYRWKFCCSEKIDTIDVNRYWLFASNSKNKLPRIISMISFSLSSLFICFRL
                     KKFKPDFLFIESPPLTLGYSGYLLSKYSGAKMIMNVSDIWPLSAKELGAIGDGKIYRK
                     LESLEKFLYGKAFINTGQSEEIVSHINNSGDFNTYLFRNGVDVKRFEKPKSYCQSGEI
                     IKIVYAGLLGVAQGIFEIVSKINFKELGAEFHIYGEGTDRKATLDFLKENPDRGIVVH
                     KSVKSNEIPEILTEYYCTIIPLVNKIYGAVPSKIYEAMAAGVPILFSGSGEGAAIISD
                     NNIGLVSSPRDFDKLKENIIKLRDSLSLRNEMSENCRKTAEEKFDRNDLIKKFSDKLV
                     SYIN"
     gene            7588..8418
                     /locus_tag="PLX80_08200"
     CDS             7588..8418
                     /locus_tag="PLX80_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20806.1"
                     /translation="MRSVFIGSKNEFDELIVDWLSEHTELAGVVWSDSATWRKTWRGR
                     FDYGMKRAKKYGYFKVLNELLLFFYVKKKVAVKDLGYLKRGIIRPYWKSNGRKFDDLK
                     FKEISSDNVNKTEVLDFLNEVKPDIIFAMCVNDFFGKKIRGIAKHGVYLWHEGLTPEY
                     KGLYSPFWTLYNGDYNNLAYSFLKMNDKIDAGEVYVSGKVRDIDLKNHNYSFIGHKAI
                     YDSLPEVEDFLKKLEAGEQKTLPDRNSESRNYTYPGITQYIKMRKRLKKYLAKNNSYK
                     "
     gene            8430..9392
                     /locus_tag="PLX80_08205"
     CDS             8430..9392
                     /locus_tag="PLX80_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY20807.1"
                     /translation="MRILITGAAGFIGYHTSERLCRDGYDVTGIDNINSYYDTDLKTG
                     RVELLEKYKNFNFKKTDLTDQEQLRKIFKEEKIDIAVNLAAQPGVRYSISNPEVSIQS
                     NIVAFFNLLECCKEFKVKKLIYASSSSVYGNSDKIPFSVKDNVDYPVSLYAATKKSNE
                     LMAYTYHHLYKFPVTGLRFFTVYGPWGRPDMAYYKFAEKIMKGESIEVYNNGNMERDF
                     TYIDDVTESISRLVKKTEDTEYRLFNIGGEHPVNLLKFIEILENKLGRNAEKIFLGMQ
                     PGDVKITSADTRDLSDYIDFVPEVRIEEGLEKFVKWYKDYYGYE"
     gene            9837..11615
                     /gene="asnB"
                     /locus_tag="PLX80_08210"
     CDS             9837..11615
                     /gene="asnB"
                     /locus_tag="PLX80_08210"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQY20808.1"
                     /translation="MCGIAGKSQWKGVSEDTIAAVSRMCNSIKHRGPDNQGVKSLGNI
                     VLGHRRLSIIDLSANANQPMSSKDERFNIVYNGEIYNFMEIKNDLIQHGYSFKTNSDT
                     EVIIYAYDKFGSDCFSMFNGMFSLAIWDSKLNELILARDRFGKKPLYYYFDKNKNISF
                     ASELKAFKEDKTFSPELSYEALNCYLAIGYILAPLTIYKNIFKLEAAHYMKISDSGNK
                     ISKFRYWDYSDSFRIKNKDSDEDTCSKILYLLEESVKRRMISDVPVGAFLSGGIDSTS
                     IVALMKKFHKGELHTFSVGFNQKSYNELPDADRAAEFIGTRHHGIICDIKNELDFLNN
                     AVDVFDEPFADNSLIPMIEVSKLASDFVTVVLSGDGADEIFAGYITYKADKYYNFAKP
                     IPDSIKRFIIGLNSNSKNKNKLNWQYKQKQFLNGALHSPEKAHYLWRQFFSPEERIEI
                     LGKEYKDLVYDTDPYNTFKKYYDIGKELDTLDRNLYVDGMTWLTDDILVKVDRTTMHS
                     SIEARSPYLDPELVEYVSSIPSNKKMKGMQLKYILKKALKDVIPEFVINKKKSGFNSP
                     VGEWIGSNGNNEFKTFNKYVYDRKFN"
     gene            11617..12387
                     /locus_tag="PLX80_08215"
     CDS             11617..12387
                     /locus_tag="PLX80_08215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20809.1"
                     /translation="MQNKQSEWSEQWTLLKDNELFLFKDWILPNTLEDFTDKEILECG
                     CGGGQHTSFIAPFAKNITSVDLNTTDIAKERNKDFKNIDFVEADIAKMDLGKKFDIVF
                     SIGVVHHTDEPDKTFENMKKHTKPGGKTIVWVYSEEGNSLVKYGVEPARKIFLKNMNR
                     KNLLKLSKFTTAILYMPVYTLYKLPLKFLPFYEYFDNFRKMSFERNTLNVFDKLNAPQ
                     VDFISKKRISDWFNSEEYDDVNINAYKGVSWSGSGVKK"
     gene            12404..13066
                     /locus_tag="PLX80_08220"
     CDS             12404..13066
                     /locus_tag="PLX80_08220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY20810.1"
                     /translation="MSFLFKYNEKLKFDTGNDNYFELLKDGFLHFSKVITFLSSPFMK
                     IVLILNGVKYGNGIKVFGLPRIENKGSVTLGNNMRLISAKCGYNSGNIAGGVFLRTSK
                     SGNIVTGDEVYLNGTSIISEESITLGNRIMIGANTVIMDTNSHNVPYKNRLKRWDKIR
                     RKAVVIEDDVWIGANCFIMKGVRIGKGSIIGAGSVVNNEVKPFSIYAGNPAVFVKKIE
                     EE"
     gene            13067..>13611
                     /locus_tag="PLX80_08225"
     CDS             13067..>13611
                     /locus_tag="PLX80_08225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20811.1"
                     /translation="MSELFTSAEQMQKATNYNQWTFEQFEKYINGDVLEVGCGVGSFT
                     KLITEKSKFDSLYCIDISAPAIDHISKKDFKGNLRIECIDLIEVNGEFDFILCMNVME
                     HVEDDVNFFAKLLSLLKPGGVLFHLVPSHNFLYSNFDKAAGHFKRYTKDKMNSLSLPV
                     DVNLIKQYYFNSFISDCKYIC"
BASE COUNT         4913 a         1823 c         2661 g         4214 t
ORIGIN      
        1 agggatttat tcatttgcaa tacttgataa cagagataag cagaaagtta ttttgtgcag
       61 agatccatca ggagtaaaac cattgtatta tttttcagaa aaaaatatca acatatttgg
      121 aagtgagatc aaagcactac ttcctacggt aaaagataaa gttagtataa atgaaaatgt
      181 cgtaaaatgt tttctgaatc ttggatattg ccctgagcca tatactatat ataatgagat
      241 taaatgtctt caacccggat atgtgattga gatttctgaa aatgagatta cggaaaaagt
      301 gatatggaat tatgaattcg aatctgaaaa caatttaagc attaaagaaa atactgaact
      361 tactgaatcg aaattaagaa ctgcagtaga aagaaattta gtagctgatg tagaagtggc
      421 agtagcattg agcggcggta tagattcttc tctcatttat gcatttgcaa atcaaagtaa
      481 cagtaatata aaagggctga cagtaaagtt tgatgatgag gaatataacg aagaagatat
      541 agcactgaag tatgtgaaaa ctcttttggg aaaacatgaa tcagtagaag tggaatctgg
      601 acttgatctt gaaacattga attttatttt gaatgatttt gatcagccgt atgcggactc
      661 atcagcagtt aatgtttttt atctgacaaa ggcagcaggg aaatatacaa aagttttaat
      721 cggcggtgac ggcggtgatg aattgtacaa cggatatccg tctcagacct ggcttacgtt
      781 tgtagaaaag atagttagta acggatttat gaaagcatcg ggaacagcat ctgtgaaaat
      841 tgcaaataag ttttcgtccg gatccaatgc aagattattt aagagaatga gagatttatt
      901 ttatgacaag ccgcatgaac ttttttatga ctggcattca tggtttccaa gaaagactga
      961 atttgaaaat ggaagtccgt ttctgtttga tacaaatgac ggattaactc tttataattc
     1021 agtgtttgct gagaagcttc cggataaatt tacaagtaaa gttgtatttg attatttcag
     1081 aaaacaaatg cttagtgatt atttaagaaa gacagacatg atgtcaatgc ttaacagtgt
     1141 tgaatacaga gtgccgatgc ttgatgaaga tctgacagaa tttgcattag gaattccatt
     1201 ctcacagaaa tcaagtttga gagaaacaaa aaaaatactc agagatattc acggaaaaat
     1261 ttatcctgct gagacaagta aaattccaaa gaagggattc atgatcccat tggacaaagc
     1321 tcttagtagt gaagaatttg aaataataaa agaagaacta ttatttaaag gaaatatagt
     1381 tttgaattat ataaaaaagg agtatgtaga attcctgttc aaatctttga agtacggagc
     1441 agaaaatgaa atcagcagag cgggtatata tcaaagaata cttatgcttt atagtttaag
     1501 tttatgggaa aaaaataaat gaagttagca attatttcac atacacctca ttacaataaa
     1561 gacggcaaga tcgttggatg gggaccgaca gtaagggaaa tcaatcacct ttcaaaaatt
     1621 tttgacagga tctaccacat tgcgccaatg cataatgaac aagctcctgg tagttcgatg
     1681 agatatgatg aagataaagt acaatacatt ccaatcaaac aatacggtgg agaaaaattt
     1741 tcagaaaagt tttcaatatt gacaacgtct tttcataatt tgaagataat cagagaggta
     1801 ttgaaaaaag tggaatgggc acaattcaga gctccaactt ctatgggact ttatgtgctg
     1861 ccatatctga gtttgatgag taagccaaaa cgctgggtaa aatatgcagg caactgggga
     1921 cagaaaaatc cacccctttc ttatagaata cagagatggt ggctgaagaa taattttcag
     1981 agatcaaaag taactgttaa cggtaagtgg aaagatgagc cagagaacat acttgatttc
     2041 agaaaccctt gtcttgataa tgaagagtta agcagagcaa aaataatttc ttcgaataaa
     2101 aattttgatg ataaaaagat actgtgtttt tcaggatcac tttcaaagaa taaaggtgta
     2161 aagttaataa ttgaagctct tgaacttatg agatttaaag ataagataga agaatttatt
     2221 tttacaggtg gcgggcatga taaggaaatg tttgagattg cagctagtaa attaaagatc
     2281 aagacaacat ttacaggatt cataagcagg aaagagcttg agaaaattta tgaaagaagt
     2341 cacatcattg tattgcccag tgagtcagaa ggatttccaa aagtaatctc agaagcagca
     2401 gcttacggat gtgtgcctat tatttctgat gtatcagcag tgaaagaata tttcaatgat
     2461 tcaaacggat tccttttgaa aagtctgaat gctgaacatc tggcagatgc gattgatctg
     2521 gcacttagta atgttgaatt aataaaatta aaatcgaggc aatgcacgga actggcacca
     2581 ttgtttacat acgaagacta tatgaaaaat ttgaaagaca aaattttaaa taattaaaca
     2641 gtaatatcat taaaatgaat atttattttc aagaaaataa attctaattt gatactgtga
     2701 aaaattctcc atttagcgcg gaaatatatg caggattaaa cgttgccatt gcaatggtga
     2761 tgctgtttaa ttcaaagctg agcgtagtat attctatcat tattttatta gcaggtgtat
     2821 atgcaatatt taagaataag aatatggacg gcgctgcgca catatatgct gcatatgtag
     2881 tagggattga agttctattg cgaatgaatt cgggaggatt tggatatgaa ttcggcaagt
     2941 atgctacgat cgtattttta gtacttggaa ttcttgtagg aaaaagaaat agaaaattac
     3001 cggtttcatt tattgcattt attttgctaa tggtaccgtc aatatttttt gttgactatc
     3061 cgacttttga tatgtcaaga caaatggttt catttaacat ttcaggaccg gtaacacttg
     3121 gtatagcagg aatttatttt tacagacgga gaattccaat acagcagctt agaaagactt
     3181 tcctggcaat gctttatcca attatagcga tgtctatatt cgtttatttc agatccggat
     3241 ctttggaaga tgtagatttt acaacagagt cgaattttca ggcaagtgga ggatttggac
     3301 caaatcaggt ttcgactatg tttggactcg gtattcttat tatagctgca gcttattttt
     3361 taaatattag gttattcaga gttaagaatc ttaatataat tttactgctg gcatttttaa
     3421 tcagaggtct tgcaacgttt tcaagaggtg gaatgcttgc accgattgtt gcaatatttt
     3481 tatgtgttgt gatcatgtca gtaacagatg catcttttca ggataaaatg aacagggttg
     3541 tatatatttt tatggcactt tttgtagtat cagtagtagg attttattat gtaaatgaag
     3601 taagcggagg tttgcttgaa atgagattta aaggcgaatc tatgtatgat aaggaaaaaa
     3661 gtaatatgtt cagcggacgc ttggagatat ttgaggaaga tatagaggta tttcaggata
     3721 atgtcctgac gggtgtagga cccggtatgg catcagaaaa aagagctgag atcagtgggg
     3781 tgtttgctgc agcacatatt gaatattcga gattacttgc ggagcacggg ctctttggga
     3841 tcatagcaat tttaataatg gtattatttc ctgttagtac attttttaaa ctgaaatcag
     3901 gtgacgaaaa gataatgctt atactatgta cggtatttgt ttttattact ttaggtcatg
     3961 ctgctatgag actcgcagct cctggattta tttacggatt agggtttatt tatatcttca
     4021 ggaaaagaag ttgatactct atgcgggtaa tattctttcg aaattcggat atacaccaac
     4081 atttatcgaa acactgacac caaaactttc tgaaagttat gacctgatat cagtttcgga
     4141 taaaaaaaat caggtattaa gaatgcttga tatgatcaaa gcagtttata aaaaccgtaa
     4201 gtcaattaaa cttgtattaa tagattcata tagtgtaaga gctttctggt acacatacat
     4261 tcttgctaag ttatgtaaaa aatttcagat accatacata cccatattgc gtggcggtgg
     4321 atatccatac aggctcaaga gcactccaga gaaatgtaaa ttcgtttttg aaaattcata
     4381 tattaatatt tcaccttctg tttttcttaa agagcatttt gaaaattcag ggtacaaagt
     4441 aacttacatt ccgaatttta taccaattga aaattataaa tataagaacc gaatctcaac
     4501 aggaccgaag atactttggg taagatcgtt tcacgagata tacaatccat tacttgcgat
     4561 tgaaatttta aaagatctga gattaaaata ttctgaagct aaactttgca tgataggtcc
     4621 ggataaggat ggttcgcttg taaaagtgga agaagcatct gtcgaaaata aattaaatgg
     4681 atcacttatt ttaacaggaa agctttcaaa aaaagaatgg acagatatat cagaagatta
     4741 tgatattttt ataaatacaa caaattttga taatcatcct gtaagtgtaa tagaggcaat
     4801 ggctctgggt ttgccggtaa tttctacaaa tgtaggagga ataccattcc ttataaaaga
     4861 cagggaagac ggagtgctag tacctgcaaa tgataaagaa aaatttgttt ctgaaattga
     4921 gaatcttatt tcagacaata aattcgcttc tgaaatttcg gctaatgcaa gaaaaaaagc
     4981 tgaagaattt gactgggatc aggttaaata taaatggttt gaagtaatag attctttagt
     5041 caagaattaa aaatcctttc gaataataca atgtcatttc tcgaattaag tctgaagcta
     5101 aagggttttc cattaagcaa agcggaaaaa gaaatacaat atctgaattc tatttcggaa
     5161 acagatttct caaaatggca gacagataag aaatgggaga tagtaaaata tcatttaaag
     5221 aataatgagt tttatagaaa taaacttgag aataaattgc ctgcaaaatg ggaagagatt
     5281 ccaatattaa aaaaaagtga ttttcaaatc tcgatagata agcttacatc agatacattc
     5341 aattcaaaag aattatacac aggttatact tcaggttcat ccggccatcc attcagatat
     5401 gcgaaagata aatattcaca tgcaatgaca tgggcattga tcaaaaacag atataatagt
     5461 tttggaataa atttgaattc aaaacaggcg agattctatg gaataccttt ggaaaagaaa
     5521 gaatattatg tagaaaagac aaaagatctt ttgtctaaca gagtgagatt cccggtgttt
     5581 gatctttctg acgaagtatt aaaaagttat atagaaattt ttaggaagac agagtttgat
     5641 tacatctatg gatattcgaa ttcgattctt ttatttgcaa gatatttaaa cagaaataaa
     5701 atcaatttaa aattaatttg cccatcttta aaagtatgta tctgcacatc ggaaaattgt
     5761 tctacagatg ataaagtcga aatttcaaaa gcttttggaa taccggtagt aaatgaatac
     5821 ggaacctcag aagttgatct gatcgcattt gaagatattg aaggtaactg gaaactttcg
     5881 gaggaaaatg tttttgtaga aattttagat gaaagcggaa agcagttaac tgaaggcggc
     5941 gaaggtagaa tcgtattaac ttctcttcat aataaggcaa tgccatttat cagatatgaa
     6001 ataggagaca gagcagtcat agaacctgga aaagacagag tcaggatcaa gaatcttctt
     6061 ggaggaatca acgatgatct cgtactgccc agcgggaaaa tctcatcaag tattacattt
     6121 tattttgtta caagaagtat tttaaataag attgattcat taaaagaatt tgtgattaag
     6181 caaactgagc cggataaatt tgtttttgaa atggtttcgg atgaagacat aagtgaaaga
     6241 gacagagagc agatcagaaa aaaaatggat gagtatttag agccgggtct tattgctgaa
     6301 tttaagagag ttgataaaat acagagaact aatgcaggaa agatgaaaca gttttattca
     6361 tttgtaaatc aggattgaaa ttagcaatac ttactcaata tttttatcca gaaaccggtg
     6421 ctcctcaaaa cagactttat gaactttcaa aaaagtttaa ggagtacggc tgggaagtaa
     6481 tggtaattac cggaatgcct aattatccaa aaggaaagat cttcccggga tacagatgga
     6541 aattctgctg ttcagaaaaa atagatacca tagacgtaaa cagatactgg ttatttgcat
     6601 caaactcaaa gaataagctg ccaagaatca tttcgatgat ttcattttca ttgtcatcat
     6661 tattcatctg tttcagatta aaaaaattca aaccggattt tctattcatt gagagtcctc
     6721 cattgacact tggatacagc ggttacttac tttcgaaata ttcaggggca aaaatgataa
     6781 tgaatgtatc tgacatctgg ccattgtctg caaaagaact tggtgcaatt ggtgatggga
     6841 aaatttatag gaaattagag tcactcgaaa aatttttata tggaaaagct tttataaata
     6901 caggacaatc agaagaaata gtatcacata taaataacag cggtgatttt aatacttacc
     6961 tattcagaaa tggagtagat gtaaaaagat ttgaaaagcc aaaatcatat tgtcaaagcg
     7021 gagaaattat taaaattgtt tatgccggat tattaggagt ggcacagggc attttcgaaa
     7081 ttgtaagtaa aattaatttt aaagaacttg gggcagaatt ccatatttat ggagaaggta
     7141 cagacagaaa ggcaacttta gattttttaa aagagaatcc ggacagggga atcgtcgttc
     7201 ataaaagtgt taagagtaat gagatacctg aaattttgac tgaatattat tgtactataa
     7261 ttcctttggt aaacaaaata tatggagcag taccatcaaa gatatatgaa gcaatggcag
     7321 ccggtgttcc tatattattt tcaggatccg gtgaaggtgc tgctataatt tccgacaata
     7381 acatcggctt agtatcatct cccagagatt ttgataagct taaggaaaat attattaagc
     7441 taagagattc attgagttta agaaatgaga tgtctgaaaa ctgcagaaaa acggctgaag
     7501 aaaaatttga cagaaatgat ttaatcaaga aattttccga taaactggtt tcatatatta
     7561 attaataatt tgggaaagag ttaacaaatg agaagtgtat ttataggatc gaagaatgag
     7621 tttgatgaat tgatagttga ctggctttca gagcacactg aactggcggg tgtagtctgg
     7681 tcagattctg ctacttggag aaagacatgg agaggcagat ttgattatgg aatgaagaga
     7741 gcaaagaagt acggatattt taaagtgctt aatgaactat tacttttttt ttatgtaaag
     7801 aaaaaagtag cagtgaaaga tctgggttat ttgaaaagag ggatcatcag accatactgg
     7861 aaatcaaacg gcagaaaatt tgatgatctg aaattcaaag agataagttc agataatgta
     7921 aataaaacag aagtactgga ttttctgaat gaagtaaagc cggatattat ttttgcaatg
     7981 tgcgtaaatg atttttttgg taagaagata agaggaattg ccaagcacgg agtatatctc
     8041 tggcatgaag gattgacacc ggagtataag ggattatact cgccattctg gactttgtat
     8101 aatggagatt acaataacct tgcatattca tttttaaaga tgaatgacaa aatagatgca
     8161 ggagaagtct atgtaagtgg aaaggtaagg gatattgatt tgaaaaatca caattactct
     8221 tttataggtc ataaagctat ttatgacagt ctgcctgaag tggaagactt tctaaagaaa
     8281 ttagaagcag gggaacaaaa gacactacct gatagaaatt cagaatcccg taattatact
     8341 tatccaggaa taacgcaata tataaaaatg agaaagcgcc ttaaaaaata tttagcaaaa
     8401 aataattcat ataaataaaa gattttaaat tgagaatact tattacaggt gcagctggtt
     8461 ttattggata tcatacttct gaaaggttat gcagagatgg atatgatgta acgggaattg
     8521 ataatataaa ttcttattat gatacagatc ttaaaacagg cagagttgaa ttattggaaa
     8581 agtataaaaa tttcaatttt aaaaaaacgg atcttacaga tcaggaacag cttagaaaaa
     8641 tatttaaaga agagaagatt gatattgcag ttaatttagc tgcacagccg ggagtcagat
     8701 attcaatttc aaatcctgaa gtgagtatac aaagtaacat agtagcattt tttaatcttc
     8761 ttgaatgctg taaggaattt aaagtgaaaa aactgatcta cgcatcatcg agttcggtat
     8821 acggaaacag tgataagatc cctttttcgg tgaaagataa tgtagactat cccgtttctc
     8881 tttatgcagc tacaaaaaaa tcaaatgaat taatggcata tacttatcat cacctttata
     8941 aatttccagt aacaggttta agatttttta cagtttatgg accatgggga agacctgata
     9001 tggcatatta taaatttgcg gaaaagataa tgaaaggtga gtcaatcgaa gtgtacaata
     9061 acggaaatat ggaaagagat tttacatata tagatgatgt aactgaatcc atttcgaggc
     9121 tagtaaaaaa aactgaagat acagaatata gattgtttaa tatcggcgga gagcatcctg
     9181 taaatctatt gaagttcata gagattttag agaataaact cggaaggaat gctgagaaaa
     9241 tatttttagg catgcaaccc ggagatgtaa aaatcacaag tgcagatacg agggatctgt
     9301 cggattatat agattttgta ccggaggtta ggatagaaga ggggttggag aagtttgtga
     9361 agtggtataa ggattattat ggatatgagt aataatgtgg gattggagaa acggttttaa
     9421 ccgtttgttt agtgaagacg gagatttttt agattaatta tgaagataag ttattgtgaa
     9481 tggtttgaag atagattttt taataagtag aattatatta aagaaacggt ttaaaccgtt
     9541 tgtttcagta caaaagtata tcttcgatga gtctatcgaa gactatgaaa aatacatttt
     9601 ataatcagat ttggtttatc cggtttcagg attttttttc acagaaacgg ttaaaaccgt
     9661 tttccacatt caaaaccgaa cccatttgaa aaatatatca aaaccatcga aatattttat
     9721 ttgtatcttg atttggttta accggtttca atattcagct ttcatagaaa cggttaaaac
     9781 cgtttctact tccatcccaa taacaaatat atcaccctac taattatctt ataaacatgt
     9841 gcggtattgc cggaaaatct cagtggaaag gcgtcagcga agatactata gctgccgttt
     9901 cccgtatgtg caattccata aaacacagag gacccgataa tcaaggcgtt aaatctttag
     9961 gaaatattgt acttggacac agaagacttt ccatcatcga tctgtctgca aatgccaatc
    10021 agccaatgtc ttctaaggat gaaagattta atattgttta taacggtgaa atctataatt
    10081 ttatggaaat aaaaaatgac cttattcaac atggctattc tttcaaaaca aactcagata
    10141 ctgaagtcat aatctacgct tatgataaat tcggttcgga ttgtttcagt atgtttaacg
    10201 gaatgttctc acttgcaatc tgggattcta aacttaatga gttgatctta gcacgggaca
    10261 gatttggaaa aaaaccattg tattattatt ttgacaaaaa taaaaatatc tctttcgctt
    10321 ctgaacttaa agcattcaaa gaagataaaa ctttttcgcc tgagttgtct tacgaagctc
    10381 tgaattgtta ccttgcaata ggatatatcc ttgcgccgct tacgatttat aaaaatatat
    10441 tcaaattaga agctgctcat tatatgaaaa tttctgattc gggaaataaa atttccaaat
    10501 tcaggtattg ggattattca gattcattca gaattaaaaa taaagattcc gatgaggata
    10561 cctgtagtaa aattttatat ctccttgaag aatctgtaaa acgcaggatg atcagtgacg
    10621 tacctgttgg agcttttctt agcggtggta ttgattcaac ttctattgta gctttaatga
    10681 aaaaatttca taaaggagaa ctacatactt tcagtgtagg ctttaatcaa aaaagttata
    10741 atgaacttcc cgatgccgat agagctgcag aatttatcgg aacaaggcat cacggaatta
    10801 tctgtgacat aaaaaacgaa cttgattttc tgaataatgc tgtagatgtt tttgatgaac
    10861 cgtttgctga taattctctc atccctatga tcgaagtttc aaaattagct tcagattttg
    10921 taactgttgt tctaagtggt gacggtgctg acgagatctt tgccggatac attacttata
    10981 aagctgataa atattataat tttgctaaac caattcctga ttctattaaa agatttataa
    11041 taggtctaaa cagtaatagt aaaaataaaa ataaacttaa ctggcagtat aagcaaaagc
    11101 aatttttaaa cggtgcacta cattctcccg aaaaagctca ttacctttgg agacagtttt
    11161 tttcacctga ggaaagaatt gaaattcttg ggaaagaata taaagatctc gtttatgata
    11221 ctgatcctta caacactttt aaaaaatatt atgatattgg taaagaactc gatacacttg
    11281 acaggaattt atatgttgat ggaatgacat ggctgactga tgacattctg gttaaagttg
    11341 acaggactac gatgcacagc agcatagaag cacgtagccc ttacctcgat cccgaactgg
    11401 tggaatatgt gtcgtctatt ccttctaata aaaaaatgaa aggtatgcag cttaaatata
    11461 ttttgaaaaa agcattaaaa gatgtaattc cggaatttgt aattaataaa aagaaaagtg
    11521 gattcaattc tccggtgggt gaatggatcg gaagtaacgg aaataatgaa ttcaagactt
    11581 ttaataaata tgtatatgac agaaagttta actaaaatgc aaaataaaca gtcagagtgg
    11641 agtgaacagt ggactcttct gaaagacaat gaactctttc ttttcaaaga ctggattctt
    11701 ccgaatacac ttgaagattt tacggataaa gaaattcttg agtgcggctg tggcggtgga
    11761 cagcatactt cttttattgc gccgtttgca aaaaatatta catctgtaga tcttaataca
    11821 actgatattg ctaaagaaag aaataaggat tttaaaaata ttgattttgt agaagctgat
    11881 attgcaaaaa tggatctcgg aaaaaaattc gacattgtct tttctattgg tgtcgtacat
    11941 catactgatg aaccggataa aacttttgag aatatgaaaa agcatacaaa gccaggtgga
    12001 aaaaccattg tctgggtata ttcagaagaa ggtaattcat tagtaaaata tggagtagag
    12061 cctgcacgaa aaatcttcct taaaaatatg aacagaaaaa atcttctaaa actttcgaaa
    12121 ttcacaactg caattttgta tatgcctgtt tatacactat ataaattgcc tttgaaattt
    12181 ctaccttttt atgaatattt tgacaacttt agaaaaatgt cttttgaaag aaatactctg
    12241 aatgtatttg ataaactgaa tgctccgcag gttgacttta tcagcaaaaa aagaatctct
    12301 gattggttta acagcgaaga atatgatgat gtaaatatta atgcttacaa aggtgttagt
    12361 tggagcggaa gtggtgttaa aaaataaaaa tttaatctga taattgagct tcctgtttaa
    12421 atataatgag aaactgaaat ttgacaccgg taatgataat tattttgaat tgctgaaaga
    12481 cggcttcctt catttttcaa aggtcattac ttttttgtct tcaccgttta tgaaaatagt
    12541 attgatctta aacggagtta aatatggaaa tggaataaaa gttttcggat tgccaagaat
    12601 tgaaaacaaa ggatctgtta ctctgggaaa taatatgagg ctgatctctg cgaaatgcgg
    12661 atataactca ggaaatattg ccggtggtgt ttttctgagg acatctaaat caggtaatat
    12721 tgtcaccggt gatgaagtct atcttaacgg aacttctatc attagtgaag aaagtattac
    12781 tctcggaaat cggatcatga tcggcgcaaa cactgtgata atggatacaa attctcacaa
    12841 tgttccatat aagaacagac tgaagaggtg ggataagatc agaagaaaag ctgtagtgat
    12901 agaggatgat gtttggatcg gcgcaaactg ttttataatg aaaggtgtcc ggatcggtaa
    12961 aggttcgatc attggagctg gaagtgttgt caataatgaa gtaaaaccat ttagtatata
    13021 tgcagggaat cctgcagtat ttgttaaaaa aatcgaagaa gaataaatgt cagaactatt
    13081 tacatccgcc gaacaaatgc agaaagccac taattataat cagtggactt ttgaacaatt
    13141 tgaaaaatat ataaatggtg atgtgcttga agtcggttgt ggtgtcggtt catttacaaa
    13201 actcattacc gagaaaagta aattcgattc tctttactgt atagatattt ctgcacctgc
    13261 gatcgatcat attagcaaaa aagattttaa gggaaatctc aggatagaat gtatagacct
    13321 tattgaagta aatggagagt ttgattttat attatgtatg aatgtaatgg agcatgtaga
    13381 ggatgatgtg aatttttttg ctaaacttct ttcactctta aaaccgggcg gggttttgtt
    13441 tcatcttgta ccttctcata attttttata ctcaaatttt gataaggccg ccgggcattt
    13501 taaaagatat acaaaagata agatgaattc actaagtttg cctgttgatg taaatttaat
    13561 aaaacaatat tattttaatt cattcatctc cgactgtaaa tatatatgtt t
//
LOCUS       DAORVQ010000085        13606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2106_length_13606_cov_6.198583, whole genome shotgun sequence.
ACCESSION   DAORVQ010000085 DAORVQ010000000
VERSION     DAORVQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_13606_cov_6.198583"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PLX80_08230"
     CDS             complement(<1..1495)
                     /locus_tag="PLX80_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20812.1"
                     /translation="MKTPSNDLWKLIRSLTVSEKGYFIKFSGRHSPGKENNYLKLFEE
                     INKQTEKYDEDIIKRKFKKERFIVQLTSAKNYLYNMILKSLVTYNEDRSTESILSNMK
                     EQYILLFQKTLFEQAEKILIRSKKLALDEEKFTKLIDIYKDQKNFDYRKIADPDFEDY
                     VESSRKEELEVLKKQINIAEYNSLYLLMSSVFKKTGIVRNKKDARDFEKIIKHPLMKN
                     ESLAKSVRAKNLFYIVSYLYYYSTSDNSRAFEFAQKRLKLIENNPLKIAGSKKEFLYA
                     LSDAIAMSYNLKRFDLCLTYLRKQREISDRSGAVSSKTPNFLDMYFKSYSFELNIYII
                     SGYFKEGLELVSEVRKWLDIYSGKLNKSEELKVLYSISYLYFGTGDFSNSLYWLNKIL
                     NDKSDIRIDYKTFAKILNLIIHYELENYDLMDYEYKSIKRYLTKKDKLYDYENLILNT
                     VKDLPGLSDKKERNFELEMLRRNITTLLKSNEINNYKALEYFDILAWI"
     gene            1714..3549
                     /locus_tag="PLX80_08235"
     CDS             1714..3549
                     /locus_tag="PLX80_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20813.1"
                     /translation="MNYIKVFITIFFITLISMHASVSKAQMFEKIDIGIPGLSHGKVN
                     WLDINNDNVLDIFVSGVTDSLSPGTFVYINNGSGNFEKILTGIPHISNASVSWSDYDR
                     DNDLDILLTGYSLNHNGVITRIYRNDLPAGFTELNFSFADIHSGSARWVDFNNDGMDD
                     ISVSGLNASGIPVFRIYKNTSGLFSDAGIILTALYNSTMEFYDFDKDGDPDLLMSGQI
                     NEFNSSYQTKIYLNENNTYIESGIVLPSMSKGSASWGDFNADGFADILINGEVGTNNS
                     SALENRVMIFHNNGDGTFSEHNASLPGIAFGNALWADFDNNGFLDILMTGNLKGNHQG
                     ITKIFINNNGIFTEVPNSLPEFTYGNVSTGDFDNDLKLDILIEGHNNQNNKNVTSIYR
                     NLIPLTNDLPVEPKGLTTTFDNNVIHFGWLPGSDPNTPAPGLTYNIKLGTTPGGVDIV
                     SPVSNGNNGYSRFPGHGNAGNNTNIHFTNLDSGTYYWSVQTVDNSLNTSDYSAEVSFK
                     YTKNNVNISSVSEIIPELFNLKQNYPNPFNPVTNILFDIAKDVNVKLNIYNSKGQLVE
                     VALNEFKKAGSYSVSFDGNNLSSGIYFYNLETNDYSETRKMLLIK"
     gene            3606..3932
                     /locus_tag="PLX80_08240"
     CDS             3606..3932
                     /locus_tag="PLX80_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY20814.1"
                     /translation="MENNLFEIPDDLDSKFEFFNTLYSDEKENGVEIEKIISAGHSTP
                     VGKWLEQEKNEWVVLITGNAEIIFSDERKFILNAGDHLFIPSGVKHRVVKTSTEPKCI
                     WLAVHF"
     gene            4191..5630
                     /locus_tag="PLX80_08245"
     CDS             4191..5630
                     /locus_tag="PLX80_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HQY20815.1"
                     /translation="MKILHKAGLGLLFILFITSHLFSQEKSQVIDELINKYYEYGQFN
                     GSILVSENGKVILSKGYGYADMEWDISNTPDTKFRLASVTKQFTAMLIMQLAEEGKID
                     LNGKITDYLQDYRKETGDKISIKNLLTHTSGIPDYTDNTVLMKKIAANPISVKDQIEK
                     LCSNDLEFDPGSQYKYSNSGYVLLGAVIEKITGKNYEEVLSEKILIPTGMTNSGYDDN
                     KKVIKNRASGYDKTLDGYVNSKYIDMSIPYSAGSMYSTVNDMYKWNQTLYTDKLLPKE
                     SIEKMFVPYLKDYGYGWHITEVEFGDAKKKLVTHSGGIFGFNTIILRYTDDKNCIIIL
                     NNFSSGNIKEMGKNISNILYDQKYDLPKISLIDILYKTYTEKSMTEAVEEFKSHMNNK
                     NDYSYREGDLNNLGYSLLQTGKIDDAIEVFRLNTEAFPNSGNTYDSLGEAYLEKGNKE
                     MAIQSYKKSVELNSENKNGIEVLNKLEVK"
     gene            complement(5780..8215)
                     /locus_tag="PLX80_08250"
     CDS             complement(5780..8215)
                     /locus_tag="PLX80_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQY20816.1"
                     /translation="MKYLLTHVLVFALMVFLTSSDFEQTSNNKSEINKNKDPLTTYSE
                     VNVNLNSKDDLKILQKNDITVEHYHGNAAEGITLVINQAELQRLKNTGISFDIVKEDV
                     SEFFKNVKIPDAADMQKSIEIQNTDNANGFSFGSMGGFYTYTEVVQKLDSMRLVYPNL
                     ISIKQNIGMSVENRAIWSVKISDNPEVNESSTEPAVYFDALHHAREPQSMASMMYYMY
                     YLLENYGVNPEVTYLLNNREIFFVPVVNPDGYVYNQTTDPNGGGFWRKNRSNNGGGCF
                     GVDLNRNYPYGWGDDLGSSNDPCSETFRGSSANSEPESQAVKNFVLQIRPKISFSMHS
                     VAGRYLNPYGYNDSAINYEIYSEFSSDFAASNNYTYGTVSEMLNYYSSGTTRDYLHSI
                     GTYAWTPEVGGTGFWNSQSQIIPIASENVYSMKYLTWVSGAFTDYQNFKILENGYIQK
                     NDTLNLEIGLKNRGLSQTAKNVSIEVTTNYPNAIPLNAIINYDSIPARQSKNNSSNPV
                     KFRLTSDANYMDEMNLFISVKQEGIQTSLDTLKLTVGKAEVLFSDNAENGSNRWISAG
                     TGIAWDSTYIDPVLGNKNFADSRYGNSADNSNNTFTLNDTIDLTNSNNPRMEFTAKWA
                     EEVTFDYSRVQVSSNFGSTWTSLTGRYTRVVSGQPSYADIKHWKNEQINLNAYIGTKI
                     RIRFTHRTNSEIPGDGFYFDNFRIVNYKDVPSSITQTSTVIPLEYRLEQNFPNPFNPK
                     TVINYELRVTSNAKLKVYDVLGNEVAELVNEKQNAGSYSVDFDGSNFSSGIYFYRLNA
                     GEFSETKRMMLIR"
     gene            8312..9070
                     /locus_tag="PLX80_08255"
     CDS             8312..9070
                     /locus_tag="PLX80_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20817.1"
                     /translation="MKTDPKERFSDRVKNYVKYRPHYPIELLTFLINECGLTESSVIA
                     DIGSGTGISAEMFIENGNMVFAVEPNMEMRKAAEKFFSGNKNFISIDTSAESTGLSEN
                     SIDFVIAGQAFHWFDAEKCKTEFKRILKDDGYLIIIWNSRIYTEGFLKEYDEFLIKFG
                     TDYVKVNHENFDDAVIKKFYQPNEVLIKKFYNFQDFDYDGLKGRLLSSSYVPNVNDLS
                     FDEMLKALEKLFDKHNSEGIVKMEYETVVYLGRL"
     gene            9228..10163
                     /locus_tag="PLX80_08260"
     CDS             9228..10163
                     /locus_tag="PLX80_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20818.1"
                     /translation="MKLKKLFKYTLILTAVIIIFVFIWFFRYRYYLLVSVDKIPEMEL
                     SSIDGYTDKMFYFNNEKIKFYLRSTEDSSSAIVTKIISPYQYEQIDTFHFNRSDQNIN
                     PDQSESGCNWELIHELDLNSNFTQGYYNVILSNSMDTFNLTITVGDVYSNNEFVVLAP
                     IATFVTYNPWGGKSLYHNYTDSTNVFFVSTQRPNTGVQYFKEDYNHIDVNVCANIFNR
                     FYENQNSNILPDYYLEERPDLFSNCKFIVLSYHCEYFSEKMYDKIEEMVFDQNIPMIS
                     LGANQIYWKIKWHDDFTVMECRKDLTFFGDGFDPG"
     gene            10164..10616
                     /locus_tag="PLX80_08265"
     CDS             10164..10616
                     /locus_tag="PLX80_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20819.1"
                     /translation="MWRHNLRSESDLLGVRYTGKGLHTFAPYEVLNSSHWLYSGFYVS
                     DVELFGLKGINSFPICGDETDKVPFWMKYKTETIAKGLNTDIADTIKIYNKDDADWNG
                     KGGGEIVLRTIGDNAVLSTGSIYSESGLGNDKVFTGFIDNYLNKYFKK"
     gene            10826..12388
                     /locus_tag="PLX80_08270"
     CDS             10826..12388
                     /locus_tag="PLX80_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20820.1"
                     /translation="MLKFFIVILISLAFTSVNAQVKQEWVSLFSNATTNQGIDLVTDN
                     SGNVYVTGLSRVNRSDILTIKYDASGQQLWSRFYNRNILKSYIGYKIGLDLNQDIVVC
                     GSESLSGAIEVVKYNSSGDTLWTRRYIRSSAISNTPINMKIDYDGSIYITLSTNNVAR
                     DDFTIVKYLTNGDLEWVHHFSLGEKFNNNANDIVLGKGDFFYLAGFSANSSVAVGLIM
                     KYNKFSGDSVWSNRTANTIFKSVILDSKEMIYACGNQSNKYYTSKIDPDGNTLWEKVH
                     YDTGGIATKIGVDSISNVYVSGYNYLHPPRISIIKYNSAGDSLWKVIESGVQNSHSTL
                     GGMIVDGEGNSYICGSNSDSSFSSTDMLTFKYNSSGNKIWLMRFNYTGTNLDFANAIT
                     GDPDGNIIVTGSCVINPNSVSTTIKYSQTVSVNNISNDIPESFSLYQNFPNPFNPKTV
                     ISYELRVKGFAKLIVYDVLGNEVSALINEKQNAGTYSAEFDGSGLASGVYFYSLNIDG
                     KNVDTKRMVLLK"
     gene            complement(12607..>13606)
                     /locus_tag="PLX80_08275"
     CDS             complement(12607..>13606)
                     /locus_tag="PLX80_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY20821.1"
                     /translation="DKKHFEKALEIDPKDIEVLLNLINILADENHEEAKMYYDKVIEI
                     DPNLANLFIEKFYNLEEAKKHFEIQLNKYPDEAFSHFNYASFLAYKLIDTEKVLKHFK
                     KAIEINSKVAIFHLSFANFLSNMVDDQKNAKIHYENAIELDPNLPEAHYELANLLFNE
                     FKEFENAKEHYEKEIKINPKNAGAFCNLALLLTNEFDDKDTAKKYYQKSLDLNPNNAE
                     VYFNFALLLTNKFEDNEGAKKYYEKAIEINPSLKNLNFKLAYLLDHNFDDYAGAKKYY
                     QKEIELNSSHAYAHFYLAILLTHKYRDKKEGQKHYLLAIGLNKDLKREEFDKYFNI"
BASE COUNT         4614 a         2060 c         2211 g         4721 t
ORIGIN      
        1 ctatccaggc aagaatatca aaatattcca aagccttata gttattgatc tcattggatt
       61 tcagaagagt ggttatgttt cttcttaaca tttccagctc gaaatttctt tcctttttat
      121 cactcagacc gggtagatct tttacagtat tcaatatcaa attttcataa tcataaagct
      181 tatctttttt tgttaaatac cttttgatcg atttatattc ataatccatc agatcataat
      241 tttcaagttc ataatgtatt atcagattca gaatttttgc aaatgtctta taatcaattc
      301 tgatatctga tttatcattt aaaattttat taagccaata caaagaattt gaaaaatctc
      361 ctgtcccaaa ataaagatat gaaattgaat acaaaacctt cagctcttct gatttattaa
      421 gcttgcctga ataaatatcc agccacttcc ggacttcact gactagttca agtccttctt
      481 taaaatatcc ggaaattata taaatgttta attcaaaact ataagatttg aaatacatat
      541 ccagaaaatt tggagttttt gatgatactg cccctgatct gtcagaaatt tccctttgct
      601 ttctcaaata tgtaagacaa agatcaaatc ttttaagatt ataggacatt gcaattgcgt
      661 cactcaaagc ataaagaaat tcttttttcg agcctgcgat ttttagcgga ttattttcta
      721 taagcttaag acgtttttga gcaaattcaa atgccctgga attatcggag gtactgtaat
      781 aataaagata actcactata taaaaaagat tttttgcgcg aacggatttt gccagcgatt
      841 catttttcat taaaggatgc ttgattattt tttcgaaatc tctggcgtct tttttatttc
      901 ttacaatacc tgttttttta aaaacggagg acattaataa ataaagagaa ttatattcag
      961 caatattaat ttgtttcttc agtacttcca gctcctcttt tcgggaactt tccacataat
     1021 cttcaaagtc aggatcagca atttttctgt aatcaaaatt cttctgatct ttatatatgt
     1081 ctatcagctt tgtaaatttt tcttcgtcta atgcaagttt ttttgaacgg atcagtattt
     1141 tttccgcctg ttcaaaaaga gttttctgaa acaataatat atactgttct ttcatatttg
     1201 ataaaatgct ctcggtcgat ctgtcctcat tataagttac caggcttttt aagatcatat
     1261 tatataaata attttttgct gaagtaagct gaacaatgaa tctttctttt ttaaatttcc
     1321 tttttattat atcttcatca tatttttcag tctgtttatt aatttcttca aacagtttta
     1381 gataattatt ctctttcccg ggtgaatgtc ttccggaaaa ttttatgaaa taaccttttt
     1441 cagacaccgt aagtgaacga ataagtttcc agagatcatt ggaaggtgtt ttcatttttt
     1501 taaatatttt taattatgtc agacatatta tatttttgag gaaattatat cattttattg
     1561 gtgttaacag ctttgtaaat taagcaaata attttccggg tatcaattca caaattttct
     1621 aataaattac atctattagc aatttctatg ttatgggttt tttaagtttt catttatgac
     1681 cttttttaac ctatattata taaaaatata tttatgaatt atataaaagt atttatcaca
     1741 atttttttta ttactttaat atcgatgcat gcatccgttt caaaagcaca gatgtttgaa
     1801 aagatcgata ttggaattcc cgggctgtca catggaaaag taaactggct ggatatcaac
     1861 aatgacaatg ttcttgatat ttttgtatca ggtgttactg attccttatc acccggaaca
     1921 tttgtttata tcaataatgg cagcggtaat tttgaaaaaa ttctgaccgg cattccgcat
     1981 atttcaaatg catcagtatc gtggagtgat tatgacaggg ataatgatct tgatattctt
     2041 ttaacaggtt attcgttgaa tcacaatggc gtgatcacaa gaatctacag aaatgatctg
     2101 cctgccggat ttactgaact gaatttttct tttgcagata ttcattccgg atcagcaaga
     2161 tgggttgatt ttaataatga cggaatggat gatatttcag ttagtggatt aaatgcatca
     2221 ggaataccgg tattcagaat ttataaaaat acatcgggat tattttcaga tgcgggtata
     2281 atattaactg cattatacaa ttctactatg gagttttatg actttgacaa agacggtgat
     2341 cctgatctgc tgatgtcagg tcagataaat gaattcaatt cgtcttatca gactaaaatt
     2401 tatttaaatg aaaataatac ttacattgaa tcgggaattg tattgccttc tatgtcaaaa
     2461 ggttctgcat catggggaga ttttaatgca gacggatttg cagatattct tataaatggt
     2521 gaagttggaa ctaataactc atctgctctt gaaaacagag taatgatttt tcataataac
     2581 ggtgacggaa cattttctga acataatgca tctcttcctg gaattgcttt tggaaatgca
     2641 ctctgggcag attttgacaa taacggtttt cttgatattt taatgacagg aaatcttaaa
     2701 ggaaatcatc agggaattac aaaaattttt attaacaata acgggatatt taccgaagtt
     2761 ccaaattccc ttcccgaatt tacttatgga aatgtatcca ccggagattt tgataatgat
     2821 cttaagttag acattttaat agagggacat aataatcaaa acaataaaaa tgttacttcc
     2881 atttacagaa atttaattcc tttaacaaac gatctgccgg tcgaaccgaa aggattgaca
     2941 actacttttg acaataacgt tatacatttt ggatggttgc cggggtctga tccgaatacg
     3001 cctgcacccg gattaacata taatataaaa ttaggtacta ctccgggcgg cgtagatata
     3061 gtatctccgg tttcaaacgg gaacaacgga tacagcagat ttcccggtca tggaaatgcg
     3121 ggtaacaata ctaatataca ttttacaaac ttagattcag gtacatatta ctggagtgtt
     3181 cagacagttg ataattcact taacacttct gattattctg cagaggtctc ttttaaatac
     3241 acaaaaaata acgtgaatat ttcttcggtt tccgaaataa ttcccgagtt atttaattta
     3301 aaacagaatt atccgaatcc atttaatccg gtaacaaata tactttttga tattgcaaaa
     3361 gatgttaacg taaaattaaa tatatataac tccaaaggac agttagtaga agtggcatta
     3421 aatgaattta aaaaagcggg aagttactca gtctcttttg acggaaataa tttatcaagc
     3481 ggtatatatt tctataatct tgagactaac gattacagtg agaccagaaa aatgttacta
     3541 atcaaatagt ggaattatct cttcagaatt ttccaacaat aaacaatctg aaaaaaacga
     3601 agtaaatgga aaataattta tttgaaatac cggatgatct tgacagtaaa tttgaattct
     3661 ttaacacatt atattcagat gaaaaagaaa atggtgtaga gatagaaaaa ataatctcag
     3721 caggacattc aacacctgta ggtaaatggc tggaacagga aaaaaatgaa tgggtagttc
     3781 tgatcacggg aaatgcagag attattttct ctgacgaaag gaaatttatt ctgaatgcag
     3841 gcgatcattt attcatccca tcaggtgtta aacacagagt agtaaagact tccaccgaac
     3901 caaagtgcat ctggcttgct gtgcattttt gaaaaacagt taatagtaag tagttaatag
     3961 ttaataattt tttaacatat gtagatttat cactgtacac ttatcacctg acaagtcaaa
     4021 atttccattt ctccaaagtc agcttgtaaa tcaaatccag aatacaggat cacttccttt
     4081 gaatacttga gtttaatgat acgatgaaat aaaatatatt tttcataaga aatgaaaccc
     4141 ttagcaaatt tttaagtcaa aataaataat ttaattttaa aaattatttt atgaaaattt
     4201 tgcacaaagc aggactgggt ttactgttca ttttatttat aacatctcat ttgttttcac
     4261 aggaaaaatc tcaagttata gatgaactga taaataaata ttacgagtac ggtcaattta
     4321 acggaagtat acttgtttca gaaaacggaa aagtgatctt aagtaaaggc tatggttatg
     4381 ctgatatgga atgggatata tcaaatactc ctgatactaa attcagatta gcttcagtca
     4441 ctaagcaatt tactgcaatg ttgataatgc aacttgcaga agaaggaaaa attgatctca
     4501 atggaaagat cactgattat ttacaggatt accgaaaaga aacaggagat aaaatttcta
     4561 taaaaaatct actgactcat acatcaggaa ttcctgatta tacggataat acagttctga
     4621 tgaaaaagat agccgccaac cccatttctg taaaagatca gattgaaaaa ttatgcagta
     4681 atgatcttga attcgatccc ggatctcaat acaagtacag taattcagga tatgtattac
     4741 ttggtgctgt tattgaaaag atcacaggaa aaaattatga agaggtactt tctgaaaaaa
     4801 ttctcattcc gaccggtatg acaaattcag gatatgatga caataaaaaa gttattaaaa
     4861 acagagcatc aggatatgat aaaacattag acggttatgt aaattcgaaa tatatagaca
     4921 tgtctattcc ctactcagca ggttcaatgt attcgactgt caatgatatg tataaatgga
     4981 atcagacatt atatactgat aagcttttac caaaggagtc aattgaaaaa atgttcgtgc
     5041 cttacttaaa agattatggt tatggatggc atattactga ggttgaattt ggtgatgcca
     5101 agaaaaagct cgttactcat tcagggggaa ttttcggatt caacacaatt attcttagat
     5161 atacagatga taaaaattgc ataattattc tgaataattt ttcaagcgga aacataaaag
     5221 aaatgggcaa aaacatttca aatatactct atgatcaaaa atatgatctg ccaaaaattt
     5281 cactgataga tatcttatat aaaacatata cagaaaagag tatgactgaa gctgtagaag
     5341 aattcaaatc tcatatgaat aataaaaacg attattctta cagagaaggt gatttgaata
     5401 atttaggata ttctttacta caaacaggca agatcgatga tgcaatagaa gtttttaggt
     5461 taaatactga ggcttttcca aactctggaa atacatatga tagtttgggc gaagcatatt
     5521 tagaaaaagg taataaagaa atggcgatcc aaagttataa aaaatctgtt gagctgaatt
     5581 cagaaaataa aaacggaatt gaagttttga ataagctgga agttaaatag aaattttatt
     5641 tagagagata ttctcttttt aaattttttt ctaaaaaaaa tccggcagag ataagttttt
     5701 gtctaagccg gattaaataa tttgtttaga ttttaactat taactattaa ctattaacta
     5761 ttaactatta actattaaat tacctgatca gcatcatcct ctttgtctct gaaaactctc
     5821 ctgcattcaa tctgtaaaaa taaatcccgc tcgaaaaatt acttccgtca aaatctaccg
     5881 aataagaacc tgcattctgt ttttcattta ctaattctgc gacttcattc ccaagcacat
     5941 catatacttt tagcttcgca ttactcgtaa ctcgtaactc gtaattgata actgtcttcg
     6001 ggttaaatgg atttggaaaa ttttgttcaa gtctatattc caatggaatg accgtgctcg
     6061 tctgagtaat tgaagaagga acgtctttat aatttacaat cctgaaatta tcaaaataaa
     6121 atccatcacc aggtatttct gaatttgtac ggtgtgtaaa tcgaatcctt atctttgtcc
     6181 caatgtatgc atttagattt atctgctcat ttttccagtg cttaatgtct gcatatgatg
     6241 gctggccgga tactactctt gtgtatctgc ctgttaagct tgtccatgta ctgccgaaat
     6301 ttgaacttac ctgaactctc gaataatcaa atgtcacttc ctcagcccac ttggctgtga
     6361 attccattct tggattattt gaatttgtta aatctatagt atcatttaat gtaaatgtat
     6421 tattggaatt atcagcggaa tttccatatc gtgagtcagc aaaattttta tttcccaata
     6481 caggatctat ataagttgaa tcccatgcaa taccagtacc tgcagagatc cagcggttac
     6541 ttccgttctc ggcattgtcg gaaaataaca cttccgcttt tccgactgtt aacttaagtg
     6601 tgtcaagcga agtctgaatg ccttcctgtt ttacagaaat aaacagattc atttcgtcca
     6661 tgtaatttgc atctgaagta agacgaaatt tcactggatt tgaagaattg tttttagact
     6721 gtctcgcagg tatgctgtca tagtttatga ttgcattcag aggaattgca tttggataat
     6781 ttgtcgtcac ttctattgaa acattttttg cagtttgcga aagtcctctg ttcttcaatc
     6841 cgatctcaag attcaaagta tcatttttct gtatatatcc attttctaaa atcttaaaat
     6901 tctgataatc ggtaaatgca cctgacaccc atgtaagata tttcatacta taaacattct
     6961 cgctggcgat gggaattatc tgtgactgtg aattccagaa gcctgtaccg ccaacttcag
     7021 gtgtccatgc atatgttcca atggaatgta aataatctct tgtagtaccg cttgaataat
     7081 aattcagcat ttctgatact gtaccataag tataattatt cgatgcagca aagtcagaag
     7141 agaactccga ataaatttca taattaattg cagaatcatt ataaccataa ggatttaggt
     7201 atctgcctgc cactgaatgc atagagaatg agatcttcgg tctgatctga aggacaaaat
     7261 tcttaactgc ctgagattcg ggttctgaat tagctgaaga tccgcggaat gtttctgaac
     7321 atggatcatt gctggatccc agatcgtctc cccacccgta tggataattt ctgttgagat
     7381 cgacaccaaa acaaccacct ccgttattac tcctgttctt tctccagaaa cctccgccgt
     7441 ttggatcagt ggtctgatta taaacataac catcgggatt tacgacaggc acaaaaaata
     7501 tttcacggtt atttaaaagg taagtgacct cgggattgac tccgtaattt tcaagaaggt
     7561 agtacatgta atacatcatc gaagccatag actgcggctc gcgtgcatga tgaagcgcat
     7621 cgaaatatac tgcaggttct gttgatgatt cattcacttc aggattatct gaaattttca
     7681 ctgaccatat tgcgcggttc tcaacgctca taccaatatt ctgtttaatt gaaatcagat
     7741 taggatatac aagccgcatg gaatcaagct tctgcactac ttcagtgtac gtataaaatc
     7801 ctcccatact tccgaaagaa aatccgttcg cgttatcagt gttttgaatt tctatacttt
     7861 tttgcatatc agcagcatca ggaattttta catttttaaa aaattcactt acatcttctt
     7921 tgactatatc aaaacttatt cccgtattct ttaacctttg caattcagcc tgattaataa
     7981 caagcgtaat gccttctgct gcattcccgt ggtaatgttc tacagtaatg tcatttttct
     8041 gtaaaatttt cagatcatct ttagaattaa gatttacatt aacttcggaa tatgttgtaa
     8101 gaggatcttt atttttattt atttcggatt tattatttga tgtttgttca aaatctgaag
     8161 aggtaagaaa aaccatcaga gcaaatacta aaacatgtgt taataaatat ttcattttat
     8221 atatatttta aaaacttaaa aaaaattatt ttaggtgtaa attagtctaa tagcaaagta
     8281 actatattga tttcaatttt aaactgacaa aatgaaaaca gatcccaaag aaagattttc
     8341 cgaccgtgta aaaaattatg tgaaataccg tcctcattat ccaattgagt tattgacatt
     8401 tttgataaat gaatgcggtc ttaccgaaag cagtgtcata gcagacatcg gttcaggtac
     8461 cggtatttca gcagagatgt ttattgaaaa tggaaatatg gtatttgctg tcgagccaaa
     8521 tatggaaatg agaaaagctg ctgaaaaatt tttttcaggt aataagaatt ttatcagtat
     8581 tgatacatct gctgaaagta cagggcttag tgaaaattca atcgactttg taattgcagg
     8641 tcaggcattt cactggtttg atgctgagaa atgtaaaaca gaatttaaac ggattctgaa
     8701 agatgacgga tatttaatta taatatggaa ttcaagaatt tacacagaag ggttcttaaa
     8761 agaatatgat gaatttttaa ttaaattcgg aacagattat gtaaaagtta atcatgaaaa
     8821 ttttgatgat gcagtgataa aaaaattcta tcagccaaat gaagttctca ttaagaaatt
     8881 ttacaacttt caggattttg attacgatgg tttgaaaggc agacttcttt caagctcata
     8941 tgtgccgaat gttaatgatc tttcttttga tgaaatgctg aaagcccttg agaaattgtt
     9001 tgataaacat aattcagagg gaattgtaaa gatggagtat gaaactgttg tttatttagg
     9061 gagactttaa ataataaata tattttgctt ttcaccctga tgccggtaga ttctgttttc
     9121 aaatagaaac atatgttact gagactattg ttaaatacgc aaattgaatt ttttttataa
     9181 aagataattt atactttgat tgaaaatatt ttccgtatta aataaaaatg aaattaaaaa
     9241 aactttttaa gtatacatta attcttacag ctgtaataat aatttttgtt tttatttggt
     9301 ttttcagata cagatattat ttgcttgttt cagttgataa aattcctgaa atggaactct
     9361 catcaatcga tggttataca gataaaatgt tttacttcaa taacgaaaag attaagtttt
     9421 atttaaggag tacagaagat tcttcctctg caattgtaac aaagatcatt tctccgtatc
     9481 aatatgaaca aatagatact tttcatttta acagatcaga tcagaacatt aatcccgatc
     9541 agtctgaatc aggctgcaat tgggaactta ttcatgagct ggatctaaat tcaaatttta
     9601 ctcagggata ttataatgta attctttcca attcaatgga tacttttaat ttaactatta
     9661 cggtaggtga tgtttacagc aataatgagt tcgtagtctt agctccaatt gcaactttcg
     9721 taacatataa tccatggggc ggaaaatctc tttatcataa ttatacagat tctacaaatg
     9781 tattttttgt ttctacacaa agaccaaata ccggagttca gtatttcaaa gaggattata
     9841 atcacataga tgtaaatgta tgtgcaaata ttttcaatcg attttatgaa aatcaaaatt
     9901 caaacatact tcctgattac tatcttgaag aaagacctga tttattttct aattgtaaat
     9961 ttatagtgtt gtcttatcac tgtgaatatt tctctgaaaa gatgtatgat aaaatcgaag
    10021 aaatggtttt tgatcagaat attccaatga tatcattagg cgcaaatcag atctactgga
    10081 aaataaaatg gcatgatgat tttactgtta tggaatgcag aaaagatctg acattttttg
    10141 gtgatgggtt tgatcccgga tgaatgtgga gacataatct cagatcggaa tcagatctgc
    10201 ttggtgtaag atacacggga aaaggacttc atacttttgc tccttacgaa gtattaaatt
    10261 cctctcattg gctgtactca ggcttttacg tttcggatgt cgagcttttt ggattaaaag
    10321 gaattaatag ttttcccatt tgcggtgatg agacggataa ggttcctttt tggatgaaat
    10381 ataaaactga aactattgcc aaaggtctga atacagatat tgctgacaca ataaaaatat
    10441 ataataaaga tgatgctgac tggaacggca aaggcggcgg agaaatagtc ttgagaacta
    10501 tcggagataa tgcggtttta tctaccggtt cgatttacag cgaatccgga cttgggaatg
    10561 ataaagtttt tacgggattt attgataatt atttgaataa atattttaag aaataaatct
    10621 gagctgtaac attgacaaaa ctgaaagtga aaattgttct tgggaatgaa gtatttgaac
    10681 ttaagctcat gtttcttttc aaataaattt aatatttttt ttttatttct attgggtcaa
    10741 gttaaaattt tttgtaagac aagttattac ttcaatttca aatacccttt tttaagttaa
    10801 taaaaaatta aacataataa catttatgct gaaatttttc attgtaattt taattagttt
    10861 agcattcacg agtgtgaatg ctcaggtaaa acaggaatgg gtttcccttt ttagtaatgc
    10921 tacaacaaat cagggaatag atctggtcac tgataattcg ggaaacgtat atgttacagg
    10981 actttcaaga gtgaaccggt cagatatttt aacaataaaa tacgatgcat ccggacagca
    11041 attatggagc agattttaca acagaaatat attaaaaagt tacatcggat ataagatagg
    11101 attggatctt aatcaggata ttgtagtatg cggttctgaa tcattatctg gcgcaattga
    11161 agttgttaaa tataattcat ccggagatac tttatggacg cgcagatata taagatcctc
    11221 tgcaatttca aacactccta taaatatgaa aatcgattat gacggtagta tttatataac
    11281 tctatcaact aataacgttg cgcgcgatga ttttacaatt gtaaaatatc tgacaaacgg
    11341 ggatttagaa tgggtacatc atttttcatt aggagaaaaa tttaataata atgctaatga
    11401 tatagttttg ggtaaaggcg atttttttta tctggcggga ttcagcgcca attcatcagt
    11461 tgcagtcggt ctaataatga agtataacaa attttccggt gattcggtct ggagtaacag
    11521 gacagctaat actattttca aaagtgttat tctcgattca aaggaaatga tttatgcatg
    11581 cggtaatcaa tcgaataaat attatacttc gaagatcgat ccggacggta atacactctg
    11641 ggaaaaagtt cattatgata ccggaggaat tgcgacaaag attggtgttg attcaatttc
    11701 aaatgtatat gtaagcggat ataattattt acatccccca agaatttcaa ttataaaata
    11761 taattctgca ggagattctt tatggaaagt aattgaatca ggggttcaga attcacatag
    11821 tactttaggc ggaatgatag tagatggtga aggtaactct tatatctgcg gatcaaattc
    11881 tgacagtagt ttttcttcca cggatatgct gacattcaag tataattcca gcggtaataa
    11941 aatatggcta atgagattca attataccgg cactaacctg gattttgcca atgcaataac
    12001 cggtgatccg gatggaaata taattgttac aggaagttgt gtcataaacc caaacagtgt
    12061 gagtacaaca ataaaatatt cacagacagt aagtgtaaat aatatttcga atgatatccc
    12121 ggaatcattt tcactgtatc agaattttcc aaatcccttt aatccaaaaa cagttattag
    12181 ttacgaatta agagtaaagg gttttgcaaa gcttatagtt tatgatgttt tgggaaacga
    12241 ggtgtcagct ttgattaatg aaaaacagaa tgcgggaact tattcagcgg agtttgacgg
    12301 gagcggtctt gctagcggcg tttattttta ttcgctgaat attgacggaa agaatgttga
    12361 tacgaagcgt atggtacttc tgaagtaatt tcaaatgtca aattagaaat gtcaaatact
    12421 aaattgaatt ttgcaattcg ctctcagtcg gattaagagg attttgaaga ttctcatcga
    12481 atacaaatct acaataattt cattcctgtg caagcgagaa actccaaatg aattgcttag
    12541 tcaaaatttt taacatttgt tataccgttt agcccgagaa tacatatctt tttgtaaacg
    12601 gtaaatttaa atattaaaat atttgtcgaa ttcttctctt ttcagatctt tatttaatcc
    12661 aatagctaat aaataatgct tttgtccttc ttttttatct ctatacttat gtgttaataa
    12721 tatagccaaa taaaaatgtg cataagcatg gctagagttt aattcaattt ccttttgata
    12781 atatttcttt gctccagcat aatcatcaaa gttatgatcc aataaatatg ctagtttaaa
    12841 atttaaattt tttaaacttg gatttatttc aattgctttc tcataatatt tcttagcacc
    12901 ttcattatct tcaaacttgt tagttaataa aagagcaaag ttaaaatata cttcagcatt
    12961 attaggattt aaatctaaag atttttgata atatttttta gcggtatctt tgtcatcaaa
    13021 ttcgttggtc aataaaagag ctaaattaca aaatgcccca gcattcttcg gatttatttt
    13081 tatttctttt tcataatgtt cttttgcgtt ttcgaattct ttaaattcat taaataataa
    13141 atttgctaat tcatagtgag cttctggtaa attagggtct aattcaatag cattttcata
    13201 gtgaattttt gcattttttt gatcgtcaac catatttgac aaaaaatttg caaatgacaa
    13261 atgaaaaata gcaactttag aatttatttc tatagctttt ttaaaatgtt ttaaaacttt
    13321 ctcagtatca atcagtttat atgctaaaaa gcttgcataa ttaaaatgtg aaaaagcttc
    13381 atcaggatat ttattaagtt gtatttcaaa atgctttttt gcttcctcaa gattataaaa
    13441 tttttctata aataaatttg ccaaattagg atcaatctct atcactttat cataatacat
    13501 ctttgcttcc tcatgatttt catcagctaa tatatttatt aaatttaaaa gaacttctat
    13561 atctttagga tctatttcta aggctttttc aaaatgcttt ttatca
//
LOCUS       DAORVQ010000086        13593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2110_length_13593_cov_5.596986, whole genome shotgun sequence.
ACCESSION   DAORVQ010000086 DAORVQ010000000
VERSION     DAORVQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_13593_cov_5.596986"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLX80_08280"
     CDS             complement(<1..772)
                     /locus_tag="PLX80_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20822.1"
                     /translation="MTPSDSTNWEIHPSFKYDTFCFINIMTGDQFYLDQYQSEYDKFK
                     NDLTPEVSQSLANLKKVIKEDNGNIVSAWLCLYYSAVGAETLSEIKFSTENISELKEN
                     FQKSPYYSDKNWAVFESVNTDIKIVLNWLDEIKFEKYWNEYIYSKVSDTINSIKNNLY
                     KYNIVGITEEHLGQKLTSDSITVYMLYYSRPHGIKITGTRFLTDIAWPFEILIRTASH
                     EMMHPPFDLKNDTLLLSKIELLKNESFFMEKVLNHNPSF"
     gene            complement(893..2431)
                     /locus_tag="PLX80_08285"
     CDS             complement(893..2431)
                     /locus_tag="PLX80_08285"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQY20823.1"
                     /translation="MKICFAASECFPFVKTGGLGDVLGSLPKIIAAENSEITEKIKND
                     QKVKTKGKKKIVKNKNEVKIIIPLYKGIKTLKYNINFMEEYGDIPVMIGDKKVTFRIW
                     TGKLPGSNVDVYFIDCPEYFHRELPYTDDPDEDERFILFQIAVIETLQRLKWAPDVIH
                     CNDWQTGLIPVYLNTNYKWDKLFEKTAVLLSIHNIGYQGRFSERSVYNAGLSYNEYYN
                     GGPYEIDNTFCFMKCGILFSEIVTTVSPTYAKEIQTPEFGAGLESVLQTRKDDLYGVL
                     NGIDKKIWNPKTDKFIPFNYSPTEFSNKKSCKKELLFEAELEYNDNIPAIGIVSRFAG
                     QKGLELIFPVINELMSLPIQLIILGSGDEGTEDFFEQLAYSFPDKVNAYIGYNNKLSH
                     YITAGCDMFLMPSVYEPCGLNQMYSLNYGTVPIVRKTGGLADTVIDFHEDSKNGNGFT
                     FEENSPSALYDTIVQALEVYNQKNIWKKIMQRGMKKDFSWDKSAKEYMSLYKKAVSKK
                     ADNK"
     gene            complement(2528..3259)
                     /locus_tag="PLX80_08290"
     CDS             complement(2528..3259)
                     /locus_tag="PLX80_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQY20824.1"
                     /translation="MNRVISKQYSPSLNKEMDIVKYGDYGFALLLFPTAAADYLEYER
                     FQLIDEIKPQIEAGKLKVYSINSINNESWLNEEMIPYDKAVRHQQFNDYVTEEVVPFI
                     FDDCNGEVPIITCGASLGALHCANLYFKRPDLFAGTIAMSGSYDLSDYSKGYFDQTCY
                     FNSPMHYLPNLEDETVLEELRKSEHIHILTGQGNYENPEASVRLSGILDKKEIKHDLE
                     LWGTDVPHDWPTWRKMVPYVIDTKF"
     gene            complement(3592..3975)
                     /locus_tag="PLX80_08295"
     CDS             complement(3592..3975)
                     /locus_tag="PLX80_08295"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HQY20825.1"
                     /translation="MDITGKLHEIYDAKQVTEKFKKREFIVEYVTNPEYPQYIKFQLT
                     QNNCDYLDGFSKGDMVEVQFDLTGRPWTNKEGETTYFTSLNAWRLQKKDGGGSSNSNA
                     DYAQSQNSSRPEVSSSNDSDSDLPF"
     gene            complement(4263..7364)
                     /locus_tag="PLX80_08300"
     CDS             complement(4263..7364)
                     /locus_tag="PLX80_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY20826.1"
                     /translation="MSISNTSINRPVLAIVMSLILIIFGLVSIVYLEVREYPSVDPPI
                     VNVTTIYPGANSEVIESQITEPLEQSLNGIEGIKTLTSESREQSSRITLEFELSRNLE
                     DAANDVRDRVSRAQRNLPPDIDNPIVQKADADATPIIFMFLQSDTKSILEVSDFATNV
                     IKERVQTIAGVSSVNIFGEKKYSMRLWLDPQRLAAYKLTPLDVQIALERENVELPSGR
                     IEGDNTELSVRTLGRFKDAEEFNELVIVQSGENTVKLKDIGYAVIGAENERTAVKRGG
                     TNGIGISVQAIPGSNIIEISDEFKKRFEVLKEEIPPEYRLEVGFDFSTFVRSTILEVE
                     ETIVIAFILVLIVIFVFLRDWRSTLIPVVAIPVSIIAGFFVIYLVGYSINVLTLVAII
                     LAIGLVVDDAIVVLENIYSKIEDGMSPMEAARKGSKEIYFAVISTTITLAAVFLPLLF
                     LEGLIGKLFKEFGVVVASTVIISSFVALTLTPMLSSRLLKKHTKSNKFYNATEPFFAG
                     MTRIYEKMLFGFMKIRWMAIVLIILMGVFVYFLKSDIQTELAPLEDRSNVRIQALGPE
                     GSTFEYTEKYMDELSQYVMDSIPEAISPITIIAPSFGSPGATNIGIVNLFLVPPEDRV
                     RTQQEIYKSLAKDLNTFTGIRGFPTMPPTIGSRFGRQPLQYVIQAPNFDKLMEVLPKI
                     LEEAKKSPKLQFVDADIKVNKPELTVSINREKASTVGVSTRDIARTLQIALGGQQISY
                     FLKGGKQYQVIAQVDREYRDDPYDLSYLFVRNNKGELIQLDNLVTVEENISPTSRLRY
                     NRYVSATIGSGLAPGVALGEAIEEMDRISAPFLDKTFSTSLAGESKDFAESSSSLIFT
                     FIFAILIIFLVLSAQFESFIDPFIIILTVPLALTGALLSLWLFDMTLNIFSQIGIIML
                     IGLVTKNAILIVEFANQRKESGLSVLEAVKSASVSRFRPIVMTSLTTILGIMPIAVGA
                     GAGSRTSLGIAVVGGLIFATFLTLFIVPAMYSYLSRKVVPATEEEIYIEDIGDIDFAD
                     IKD"
     gene            complement(7411..7908)
                     /locus_tag="PLX80_08305"
     CDS             complement(7411..7908)
                     /locus_tag="PLX80_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY20827.1"
                     /translation="MLANIQETGRVKLDFSVPEKYVTAFNKGQTITFSVEGESEEFTG
                     TIAAFEPKLDGTTRSLTVRAITQNPGRKLLPGTFANVNLELNTIKDAIMIPTQALIPK
                     LKGHDVFVLKNGSALLTDVLTGVRTDNQIQILSGISAGDTILTTNILRLKSGSPVVID
                     IMESQ"
     gene            complement(7932..8489)
                     /locus_tag="PLX80_08310"
     CDS             complement(7932..8489)
                     /locus_tag="PLX80_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQY20828.1"
                     /translation="MSKKIKYISVSLIIILIAAAFILPKLLSGDKNEIDKKPGPDSKN
                     MPTPANGYIVNAEELDNSIKVIGSIVANEEVELRSELSKKITGIYFKEGTYVSKGKLL
                     FKLDDSDLDAQMNKLDIQSEIIEKNLSRIQQLYDKNLSTSEEMDNLQNESDRVNAEME
                     ILEIQIDKTNIRAPFSGIIGFKMSA"
     gene            8889..9464
                     /locus_tag="PLX80_08315"
     CDS             8889..9464
                     /locus_tag="PLX80_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20829.1"
                     /translation="MNGAHLHLILNHIPVIGTIFAMFILFIGIIKKSDDVKKVCMLVI
                     IFTSILTIPVYLTGDKAEEMIEGNYEDVDEEFIHSHEDFALYSFIAMNIMGAVALISM
                     FIYKKPKLLPNSFAYFMFALLIIVNGMMTYTANLGGKIHHPEIREDKLPWESNVIQDE
                     KNDDRSGKNEHKEKEDELEEEQNDHKGHDHN"
     gene            complement(9477..10460)
                     /locus_tag="PLX80_08320"
     CDS             complement(9477..10460)
                     /locus_tag="PLX80_08320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQY20830.1"
                     /translation="MERNYTFIVPEVKTKQRIDKYIANFIENASRTKVKKAIDLGFVT
                     VNGDLVKSNYIVKPEDEIEIELDYPEKQDILPEDIPLDIVFEDEYLMIINKPPGMVVH
                     PAYKNYTGTLVNAILYYAEKTKGSLSNLNGFERAGIVHRLDKDTSGLIVIAKDELTHR
                     KLSEQFFHHTIEREYAAIIWGKLKKKKGEISNRLGRDKRDRKKVTVLKDDDASGKLAI
                     TNYEVISEYDFLSLIKLNLKTGRTHQIRVHMADIAHPVFGDETYGGREPHSVNLSSNL
                     KARIKNLLEKIDRQALHARVLGFVHPITNEKIRFESELPDDMKNVIAEITT"
     gene            10640..13402
                     /locus_tag="PLX80_08325"
     CDS             10640..13402
                     /locus_tag="PLX80_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQY20831.1"
                     /translation="MQNKFIFKKARSSYFTFLILLTFSVSVYMSGCSSSEDEVEVTQR
                     MIDSMDVYTQIDNSFAVYQKALNYNQSANSKEAGESFESSLKILSNINSNIINNSEYY
                     AWKIDYDELAVSIVQDYISTQSEISANSLAFDFAKKNAIKYEKVEEVSGDRAALPDGS
                     DLPLDKNSAVDGYIEFFTNTDRGKSFIDKTLYRSGKFFPLMRKILKFNNAPEELIYLS
                     VQESGLAPTIMSKAGAVGLWQFMPATGYAYGLGQDEYRDDRMDMEKSTDAAARHLKDL
                     YRSLGDWYLAFAAYNAGPGRVTKAIKKSGSRDFWTLRGFLPGETKNYVPSILALSFVF
                     RDPEGYGFKNVEYGTPISFDRVNVNTSMTLQQVADMCDADVETIRELNSELTQDMVPD
                     YQDYYQLRIPHKTYDKFIANYNDATYLDKSGSKSPEFAGTEKGGYEQMIELSYYKVKN
                     YDPGNIQNIGSTVGKKKMTYQFKKKDALEAIAVFYDVRPVEIRIWNNIPYGSPIKQQQ
                     NLNIYVTEEKYNLLNGLNQNGIDKSKKIDENDINQTDKNSVAEEVEEDVKKKDSNYSD
                     NTKTTDSKNTDTQSSTNKRYVKKKKGSESTETKTNDSKVVDNNSTVTKVDLPKKSGET
                     DELKNSDDNSDKEKNSDENSDEDNKYISKAEENQEENTSGENDNSISIENNNEKDATE
                     ETVEVQKSEEDIARDSFLSANNPSNVMVTNYTTERSTVNEKQDESTGNYSYNSTEIVK
                     APEEKKVTETKKKTSTKSKGKTTYYTVSEGDNLSDIADDHNVSINDLKEWNDLDSDKI
                     LVGQKLKLQGGSSSKSKTSTHTVTSGENLTMIADEYNMSVSDLKEINDLSSDVIMTGQ
                     KLKVKGSKSGKNTNSGNKSSHKVKSGETLASISDKYNVSISNLRKWNKLKSDKILIGQ
                     VIKINP"
BASE COUNT         4441 a         2394 c         2285 g         4473 t
ORIGIN      
        1 caaacgaagg gttatgattt aaaactttct ccataaaaaa actttcattt tttagtaact
       61 cgatcttact caaaagtaaa gtatcatttt ttagatcaaa tgggggatgc atcatttcat
      121 gggaagctgt tcttataagt atttcaaacg gccatgcaat atcagttaga aatctagtac
      181 cagtaatttt tattccatga ggacgtgaat agtaaagcat ataaactgta attgaatccg
      241 atgttaattt ttgtccgaga tgctcttccg taattccgac aatattgtat ttatacagat
      301 tattttttat tgaattaatt gtatcagata ctttgctgta aatgtattca ttccagtatt
      361 tttcaaattt gatctcatcc agccagttga gaactatctt aatgtcggta ttcacagatt
      421 cgaaaactgc ccagttttta tcagagtaat atggcgattt ttgaaaattt tcttttagct
      481 cagaaatgtt ttctgtacta aattttattt cagaaagagt ctcagctccg actgccgaat
      541 agtaaagaca aagccatgct gatactatgt tcccattatc ttctttgatc acttttttga
      601 gatttgccaa agattgagat acctccggag tgaggtcatt tttgaattta tcatattcac
      661 tttggtattg gtccagatag aactgatctc cggtcataat atttataaaa cagaatgtat
      721 catacttgaa tgaaggatga atttcccagt tagtggaatc tgatggcgtc attgaataag
      781 aattatttat taaaataaat gagattagta atattaagaa cttcattata atgataatat
      841 tttttctaat tatatcgaac ttaagaataa aaaagttatg taataattaa aattacttat
      901 tatcagcttt tttggaaaca gcttttttat aaagtgacat gtattccttt gcagatttat
      961 cccacgaaaa atctttcttc attcctctct gcataatttt tttccatata ttcttttgat
     1021 tatatacttc aagagcttgg acaattgtat catataatgc agatggagaa ttttcttcaa
     1081 aagtaaaacc attcccattc ttagaatctt catgaaaatc aatcactgta tctgcaagtc
     1141 ctcccgtctt tcttactata ggaactgtac cgtaattcag actatacatt tgattcaatc
     1201 cacatggctc atagacagac ggcattaaaa acatatcaca gcctgcagtt atgtaatgtg
     1261 aaagtttatt attatacccg atgtaagcat ttactttatc aggaaaactg taagcaagct
     1321 gctcaaaaaa atcttcggtt ccttcatcac cgctgccgag tattataagc tgaattggta
     1381 aactcattaa ttcatttatt acaggaaaga tcagctcaag tcctttctga ccggcgaatc
     1441 tcgacactat accaatagcc ggtatattat cattgtactc aagttcagct tcaaagagaa
     1501 gttctttttt acatgatttt ttatttgaga attctgtcgg gctgtaatta aatggtatga
     1561 acttatcggt tttcggattc cagatctttt tatcaattcc atttaatact ccatagagat
     1621 catcttttct tgtttgcaaa acactttcta aacctgcgcc aaattcaggt gtctgaattt
     1681 cttttgcata ggtaggactt actgtagtta caatttcgga aaaaagaatt ccgcatttca
     1741 taaaacaaaa tgtattatca atttcatacg gaccaccatt ataatattca ttataagaca
     1801 gacctgcatt ataaactgat ctttcggaga atcttccctg atagcctata ttgtgaattg
     1861 ataaaaggac tgcagttttt tcaaacagct tatcccattt ataattagta tttaaataaa
     1921 caggaataag tcccgtctgc cagtcattgc agtgaattac gtccggtgcc cacttaagtc
     1981 tttgaagagt ttcgatgaca gcaatctgaa aaagtataaa tctttcatcc tcatcgggat
     2041 catcggtgta tggcaattca cgatgaaaat attcgggaca gtcaataaaa taaacatcta
     2101 catttgatcc gggtagctta cctgtccata tcctgaatgt tactttttta tcaccgatca
     2161 ttacaggaat gtcaccatac tcttccataa aatttatatt atacttaaga gttttgatac
     2221 ctttatataa tgggataatg atctttactt catttttatt cttaacaatt tttttctttc
     2281 cttttgtttt tactttttga tcattcttta tcttttcggt tatttcagaa ttttcagcgg
     2341 caatgatctt cggcagtgat ccgagcacgt ctccaagtcc gcctgtctta acaaacggaa
     2401 aacattctga agctgcaaaa catatcttca atgtatattc tatttttttt aaataaaaag
     2461 attactatag gataactgaa taaaatactg tttgacaaaa ttgttaaaca gtattttaat
     2521 tttgatttta gaattttgtg tcaatcacat aaggaaccat ttttctccag gtaggccagt
     2581 catgcgggac atcagttccc caaagttcaa gatcatgttt gatttctttc ttgtcaagaa
     2641 ttccggataa tcttacagat gcttccgggt tttcgtaatt tccctgcccg gtaagaatat
     2701 gtatgtgttc actttttctt aattcttcta gaacagtttc atcttcaaga tttggaaggt
     2761 aatgcattgg tgaattgaaa taacatgtct gatcaaaata acctttggaa taatcgctca
     2821 gatcataaga tccgctcatt gcaattgttc ctgcaaaaag atcaggtctt ttgaaatata
     2881 gattagcgca gtgcaaagct ccgagagatg ctccgcatgt gatgatcgga acttcaccgt
     2941 tacagtcatc aaaaataaac ggcactactt cttcagtcac ataatcattg aactgctgat
     3001 gtcttacagc tttgtcatat ggaatcattt cttcattgag ccagctctca ttatttatac
     3061 tgttaatgga gtagaccttt aatttgccgg cctctatctg cggctttatt tcatctatta
     3121 attgaaatct ttcatactca aggtaatcgg cagcagcagt aggaaaaagt agtaatgcaa
     3181 atccgtaatc accgtatttt acaatgtcca tttctttatt cagactcgga ctgtattgtt
     3241 ttgatatcac tctgttcatt tgattaattt aatttggttt gaaaatttat aaggactgca
     3301 agataataat tttgagaatt aattatcagt ctgaatttag tgctgaaagt gataaattgc
     3361 tttgttattt atagtggaaa tctagccgtc gcatctgtca aagtcaagtt tccgattcta
     3421 gttggtcacg ttcgttccgc tctcttgtga caaacagcgg ctcaataaat acttttggtt
     3481 ataaactggc ggatgtgagt ttgaaacgga gtttcaaact cagctgcgtt cccaaacaag
     3541 gagtttggga acgagtgaat gtatattttt taagataaaa tttaagctat atcagaatgg
     3601 aaggtcgctg tctgaatcgt tgcttgaaga aacttccggc cgactgctgt tttgactctg
     3661 cgcataatca gcattactat tactgcttcc gccgccgtct tttttctgaa gtctccatgc
     3721 gttcagactt gtgaaatatg tggtctcgcc ttctttatta gtccatggtc tgccggtcag
     3781 atcgaactgt acttccacca tatctccttt actgaatccg tccagatagt cacagttatt
     3841 ttgtgtcagt tgaaatttta tatattgcgg atattcggga ttggtaacat actcaacgat
     3901 gaattctctc tttttaaatt tttccgtaac ctgttttgca tcatagatct catgcagttt
     3961 tcctgtaatg tccattattt tttttttgat taaattatta taaaataaaa atctgataag
     4021 attcagatca acagcaaatt aattttatcc gattaaactg acaagtcaat atattaaata
     4081 tttaatttta aattttaaat attaaatgtc tgacagactt ttcttattta aataacaaaa
     4141 acctgcctca aagtcccaaa ggttcaaagg ttaagtctcc acagagttgc gtgtttatat
     4201 gtctccgcag agtcacgtgt tcatcggact ctgcggaaag tcaatattta aaattaaatg
     4261 aattagtctt taatgtcagc gaagtcaata tcaccaatat cttcaatata aatttcctct
     4321 tcagtcgccg gtacaacttt ccttgaaaga tatgaataca tcgccggtac aataaataaa
     4381 gtaaggaacg tcgcaaaaat aagtccaccc actactgcta taccgagtga agttctgctg
     4441 cctgcacctg cgccgactgc aatcggcatt attccgagaa tagttgtaag acttgtcatc
     4501 acgataggtc tgaatcttga tactgatgca ctctttactg cttcgagtac actcagccct
     4561 gattcttttc tctgattcgc aaactcaact ataagtattg cattctttgt cacaagtccg
     4621 atcagcataa taattccgat ctgtgaaaag atattcagcg tcatgtcaaa taaccataat
     4681 gaaagcaatg ctccggtaag tgcaagtgga acagtaagga ttattatgaa cggatcaata
     4741 aaactttcga actgagccga caatacgaga aagattatca ggattgcaaa tataaaagtg
     4801 aatataagac tcgacgaact ttcagcaaaa tcttttgatt cacctgcaag tgatgtagaa
     4861 aaagtcttat caagaaatgg agcagagatc ctgtccattt cttcaattgc ttctccaagc
     4921 gctacacctg gtgcaagtcc gctgccgata gtagctgaga catatctgtt atatcttagt
     4981 cttgaagtcg gactgatatt ttcttcgact gtaactagat tatcaagctg tataagttca
     5041 cctttattat ttctaacaaa taaataactc agatcataag gatcatctct gtattccctg
     5101 tccacctgcg caatgacctg atactgcttt ccgcctttta gaaaataact tatctgctga
     5161 ccaccgagtg cgatctgaag tgtccttgca atatctcttg tggagactcc gactgtagat
     5221 gctttctccc tgttaattga gacagtaagt tcaggtttgt ttactttaat atcagcatcg
     5281 acaaactgca gcttcggact tttctttgct tcctcaagaa tctttggcag cacttccatc
     5341 agcttatcaa aattcggtgc ctgtatcaca tactgaagcg gctgtcttcc aaatctcgag
     5401 ccgatagtcg gcggcattgt aggaaaacct ctgatccctg taaaagtatt cagatctttt
     5461 gcaagacttt tataaatttc ctgttgagtc ctcactctgt cctcaggtgg tacgagaaat
     5521 aaatttacta taccaatatt tgtagcaccc ggtgaaccaa agctcggagc aataattgta
     5581 atcggactta tagcttcggg aattgaatcc atgacatact gggaaagctc atccatgtat
     5641 ttttctgtgt attcaaaagt cgatccttca ggaccaagtg cctgtatccg aacatttgat
     5701 ctgtcctcta aaggtgcaag ttctgtctga atatctgatt tcagaaagta aacaaatact
     5761 cccataagaa ttattaatac aattgccatc catctgatct tcataaatcc aaaaagcatt
     5821 ttctcataaa ttctagtcat gcctgcaaag aaaggctcag tggcattata aaatttattg
     5881 gatttagtat gttttttcaa aagtcttgaa gacagcatcg gggtaagtgt caatgcaaca
     5941 aatgaagata tgataactgt agaagcaacg actacaccaa attctttgaa cagcttgccg
     6001 ataagtcctt ccagaaaaag taacgggaga aatacagcag caagtgtaat ggtcgtcgag
     6061 ataacagcaa aataaatttc ctttgaacct tttcgggcag cttccattgg agacattcca
     6121 tcttcgatct tggaataaat attttctaat actacaatag catcatctac aacaagtccg
     6181 attgcaagaa taatcgctac aagtgtaagt acatttatag aatagcctac aagatatatc
     6241 acaaaaaatc cggcgatgat agaaacagga attgcaacta caggaatcag cgtagatctc
     6301 cagtcacgaa ggaatacaaa tattacaatc aatacaagta tgaaagcaat aacgatagtc
     6361 tcttctactt ccaaaatggt cgaacgcaca aatgtagaaa aatcaaaacc tacttctaat
     6421 ctgtattcag gaggaatctc ttctttaagt acttcaaatc tttttttgaa ttcatcagag
     6481 atctctatta tatttgatcc gggtattgcc tgaactgaaa ttccaattcc attggttccg
     6541 cctcttttaa cagcagtcct ttcattttca gcaccaataa ctgcatagcc gatatctttc
     6601 agctttacag tattttcacc gctttgtaca attaccagtt cattgaattc ttcagcatcc
     6661 ttaaatctgc cgagtgtccg gacagacaat tcagtattat caccttctat tcttccggat
     6721 ggaagttcta cattttctct ttcaagagcg atctgtacat caagcggtgt aagtttatat
     6781 gctgccagtc tctgcggatc gagccataat ctcatcgagt attttttctc accgaaaata
     6841 tttactgaac tgacacctgc tattgtctga actctttcct tgataacatt tgttgcaaaa
     6901 tcacttacct ccaggatact ttttgtatca ctttgcaaaa acataaatat gatcggcgtt
     6961 gcatcagcat cggctttctg aactattgga ttatctatgt caggaggaag attcctctgc
     7021 gctctggaaa ctctgtccct tacatcattt gctgcatctt cgagatttct gctgagttca
     7081 aattcaagag ttatcctgct ggactgttcg cggctttcag aggtcagagt ttttattcct
     7141 tcgattccgt ttaatgactg ttcgagcggt tcagtaatct gggattctat tacttcggaa
     7201 tttgcacccg gatatattgt agtaacgttt acaatcggcg gatcgacgct gggatattct
     7261 cttacttcaa gatagacaat tgagacaagt ccgaatataa taagtatcag cgacattacg
     7321 atcgcaagta cgggacggtt tatggaagta tttgagatag acatattata ctattattag
     7381 atataaagaa ttgaatttta aatataaatt ttactgtgat tccattatgt caattacaac
     7441 aggcgaaccg gatttgagtc tcaggatatt agtagtaaga attgtatcac ctgcacttat
     7501 accggacagg atctggatct gattatctgt tcttacacct gtcaatacat ctgttagaag
     7561 tgcggatccg ttttttaaaa caaatacatc gtgaccttta agctttggta tgagagcttg
     7621 cgtaggtatc atgatcgcat cttttatagt gttcagttct agatttacat ttgcaaatgt
     7681 accgggcaat agttttcttc ccggattctg agtaattgct cttacggtca gtgaccttgt
     7741 ggtgccgtca agcttaggct cgaatgctgc gatagttcct gtaaattctt cactttcacc
     7801 ttcgacagaa aacgtaatag tctgcccttt attaaaagct gtcacatatt tttccggtac
     7861 ggaaaaatcc agtttcactc ttcctgtctc ctgaatattt gccagcacta ctgaaggagt
     7921 gacataactt cctatgctga cattttaaat cctataatac ctgaaaaagg tgcgcggata
     7981 tttgttttat ctatttgtat ctcaagaatt tccatctcag catttaccct gtcagattca
     8041 ttttgcagat tatccatctc ttcacttgta gaaaggtttt tatcataaag ctgctgaatc
     8101 ctgctaagat ttttttcaat gatctcactt tgtatatcaa gcttattcat ctgagcatcg
     8161 agatcagagt catctaattt aaaaagtagt ttgcctttcg agacataagt tccttcttta
     8221 aaataaattc ctgtgatttt ttttgaaagt tcgcttcgca gctctacttc ttcatttgca
     8281 acgatagagc caatgacttt aatagaattg tcaagctctt ctgcatttac aatatatccg
     8341 ttggcgggtg taggcatgtt ttttgaatca ggtcccggct ttttatcaat ttcgttttta
     8401 tctccggaaa gcagctttgg caaaataaaa gcagctgcaa ttaaaataat tattaaactt
     8461 actgaaatgt atttgatttt tttactcaaa ggttttattg attttttata ttttaattta
     8521 ctttttaaaa attttaattt aaatgtaaat tcttaaacag ataattaacc aaccactata
     8581 aggtagctga caaaacaaaa actgataagg tttaacagaa tttaaactga tttatttata
     8641 aagaaatttt caataaatta attccatttt ttcaaaaaac atttaaaagg ttttattcaa
     8701 aaaaattcaa tatttaataa ttagtcaata ttcaacattg aatcctacat ttacagaatt
     8761 tttttatttc agattaataa attaaatgtt taattttgta atacaaatta ttcatttata
     8821 acaaaaatga aattgtatat tttaaaccta atgatattta aattttagaa aaggagtctt
     8881 gctagattat gaatggtgca catttacatt taattctcaa tcatatccct gtcatcggta
     8941 caatcttcgc aatgtttatt ttatttattg gtataataaa aaaaagtgat gatgtaaaaa
     9001 aagtatgtat gctggtaata atttttactt ctatacttac gatacctgtt tatctgaccg
     9061 gtgacaaagc tgaagaaatg atagaaggaa attacgaaga cgtggatgaa gaatttatac
     9121 attcacatga agattttgct ttgtatagtt ttattgcaat gaatatcatg ggagcagtcg
     9181 cacttatctc aatgttcatt tataagaaac caaagctctt accaaattct tttgcatatt
     9241 ttatgtttgc tttactaatt attgtaaatg gaatgatgac ttatactgcc aatctcggag
     9301 gtaaaattca tcatcctgaa atacgcgaag acaagcttcc atgggaatca aatgttattc
     9361 aagatgaaaa aaatgatgac aggtccggga aaaatgaaca taaagaaaaa gaagatgaac
     9421 ttgaagaaga acaaaatgat cataaaggtc atgatcataa ttagatcaaa gttcttttat
     9481 gttgttattt cagctataac atttttcata tcatcaggca gctcagactc aaatctgatc
     9541 ttttcatttg ttatcggatg aacaaaccca agtactctcg catgcagtgc ctgtctgtca
     9601 attttctcca aaagattttt tatacgggct tttaaatttg acgaaagatt tactgaatga
     9661 ggttctctgc cgccgtaagt ttcatctcca aaaacaggat gagctatgtc agccatatga
     9721 acccggatct gatgcgttct gcccgtcttt aaatttaatt tgatcagtga aagaaaatca
     9781 tattcactga tcacttcata atttgtgatt gcaagtttac ctgaagcatc atcatctttt
     9841 aaaacagtaa ctttttttct gtccctttta tctctgccaa gccggttcga gatctctcct
     9901 tttttctttt ttaattttcc ccagattatt gcagcatatt ctctttctat agtatgatga
     9961 aaaaactgct ctgacaattt tctatgagta agttcatctt tggcaattac gatcagccct
    10021 gatgtatctt tatcaagcct gtgaactatt cctgctcgtt caaaaccatt cagatttgat
    10081 aaacttcctt ttgtcttttc agcataatat aaaatagcat ttaccagtgt accggtgtaa
    10141 tttttatatg cgggatgaac gaccatgccg ggtggtttat ttatgatcat cagatattca
    10201 tcttcaaata cgatatcaag cggaatgtct tcaggcagaa tgtcctgctt ttcaggataa
    10261 tcaagttcga tctcaatttc atcttcaggc tttacaatat aatttgactt aacgagatct
    10321 ccgtttacag ttacaaatcc tagatcgatt gcttttttta ctttagtacg cgaagcgttt
    10381 tcaataaaat ttgcaatata cttgtcaatt ctttgcttcg tttttacttc aggtacaatg
    10441 aatgtataat ttctttccaa gctatttttt taatattaca aatatatact cttgaattta
    10501 tgaattaact gtaaaaattc ttaaattaaa gtttacactg aattttaaaa aaactttatt
    10561 tattttacaa acagttaaaa cttaacttta cttaaaatat ctaagctatt tatttttatg
    10621 aaatttaaat tttagaatca tgcaaaacaa attcattttt aaaaaagcaa gatcttcata
    10681 tttcacattc cttatattat taacattttc ggtttcagta tatatgtcgg gctgttcatc
    10741 atctgaggat gaagtagaag ttactcagag aatgattgat tcgatggatg tgtacactca
    10801 gattgacaat tcatttgctg tttatcaaaa agcacttaat tataatcaaa gcgcaaacag
    10861 taaggaggca ggagaatcgt ttgagtcctc attgaaaatc ttgagcaata ttaacagtaa
    10921 tatcataaac aattccgagt attacgcctg gaaaattgat tatgatgaac tcgctgtaag
    10981 tatagtacag gattatattt ctactcaaag tgagatcagt gcaaatagtc ttgcatttga
    11041 ttttgcaaaa aagaatgcga taaaatatga aaaagtagaa gaggtaagcg gtgaccgtgc
    11101 tgcacttcct gatggaagtg atcttccgct tgacaaaaat tcagcagtag atggatatat
    11161 agaatttttt acaaacactg acagaggtaa aagttttatt gacaagactc tgtatcgctc
    11221 aggaaaattc ttcccgttaa tgaggaagat tttaaaattc aataatgcac ctgaagagct
    11281 tatctatctt tcagtacagg aatcagggct tgctccaacc ataatgtcaa aagccggcgc
    11341 agtaggactc tggcagttta tgccggctac aggttatgct tatggtctgg gacaggatga
    11401 ataccgtgat gacagaatgg acatggaaaa atcaacagat gctgcagcaa gacatcttaa
    11461 agatctttac agatcacttg gtgactggta tctggcattt gctgcatata atgcaggacc
    11521 aggcagagtt accaaagcga ttaaaaaaag cggatcgaga gatttctgga cattgagagg
    11581 atttctaccc ggtgaaacaa aaaattatgt gccttcaata cttgcactat cttttgtttt
    11641 cagagatccg gaaggatatg gttttaaaaa tgttgagtat ggcacaccga tttcttttga
    11701 cagggtaaat gtcaatacat ctatgactct tcagcaggtc gcagatatgt gtgatgcaga
    11761 tgttgaaact atcagagaat tgaattcaga actgactcag gatatggttc cggattatca
    11821 ggattattat cagctcagaa tccctcataa gacttatgat aaatttattg ccaactataa
    11881 tgatgcaact tatttagata aaagcggcag taagagtccg gaatttgccg gtacggaaaa
    11941 aggtggatat gaacaaatga tcgaactttc ctattacaaa gtcaaaaact atgacccggg
    12001 taatattcaa aacattggca gcacggttgg aaaaaagaag atgacatatc agttcaagaa
    12061 aaaagatgcg ctcgaagcaa ttgcagtttt ttatgatgtc agaccggtgg agatcagaat
    12121 ctggaataac attccttatg gttcgcctat aaaacaacag cagaatctca acatttatgt
    12181 cacagaagaa aaatataatc tgcttaacgg attaaatcaa aatggaatag ataagtctaa
    12241 aaagatagat gaaaatgata tcaatcaaac agacaagaac tccgttgctg aagaagtaga
    12301 agaagatgtt aagaaaaaag attctaatta ttcggacaac actaaaacta ctgatagcaa
    12361 aaacactgat actcaaagca gcactaacaa acgttatgta aaaaagaaaa aaggatctga
    12421 aagtactgaa acaaaaacta atgattcgaa agttgtcgac aataacagta ctgtaacaaa
    12481 agtggatctt ccaaagaaaa gcggtgaaac tgatgaactg aaaaatagtg atgacaacag
    12541 tgataaagag aaaaatagtg atgaaaattc tgatgaagat aataaataca tcagtaaagc
    12601 agaagagaat caagaagaaa atacttccgg cgagaatgac aatagtatca gcattgaaaa
    12661 taataatgaa aaagatgcaa ctgaagaaac agtcgaagta caaaagtcag aagaggatat
    12721 tgctagagac agcttcttaa gtgcaaataa tccatcgaat gtaatggtta caaattatac
    12781 cacagaaaga agcacggtaa atgaaaagca ggatgaaagc acaggcaatt attcttacaa
    12841 tagtaccgaa atagtaaaag cccctgaaga aaaaaaggta actgaaacaa aaaagaaaac
    12901 cagtacaaag tcaaaaggta agacaactta ttatactgtt tccgaaggtg acaatctatc
    12961 ggatattgct gatgatcata atgtttcaat taatgatctg aaagaatgga atgatctcga
    13021 ctctgacaaa attcttgttg gtcagaaact caaactacag ggaggatctt cttcaaagtc
    13081 taaaacatct actcatacag ttacatccgg tgaaaatctt actatgatag cagatgaata
    13141 taatatgtca gtttctgatc tgaaggaaat aaatgatctg agcagtgatg taataatgac
    13201 cggacagaaa ttaaaagtaa aagggagtaa atcaggtaag aatactaact cagggaataa
    13261 gtcttcgcat aaagtaaaaa gcggtgaaac acttgcttca atatccgata agtataatgt
    13321 ttctatttct aatctaagaa aatggaataa acttaaaagt gataagatac taataggtca
    13381 ggtaataaaa ataaatcctt aacagtcaag taaatttaaa gctaaaaaaa aaaaattaaa
    13441 gaatctttaa actgaattga aagaaatata tataagatat atattgcatt cgtttaaaag
    13501 acaacacatc aaaaaagtat aaaggaattg ctggcatagc tcaattggta gagcagctga
    13561 tttgtaatca gcaggttggg ggttcaagtc cct
//
LOCUS       DAORVQ010000087        13564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2118_length_13564_cov_4.729218, whole genome shotgun sequence.
ACCESSION   DAORVQ010000087 DAORVQ010000000
VERSION     DAORVQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2118_length_13564_cov_4.729218"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..556
                     /locus_tag="PLX80_08330"
     CDS             326..556
                     /locus_tag="PLX80_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20832.1"
                     /translation="MKTENISNLTFDRLLELVRQLSFQEKIILSKELEKECIDSKLSS
                     LLKTFKTNELSEDTINEEVEYVRDQMYERKKH"
     gene            537..959
                     /locus_tag="PLX80_08335"
     CDS             537..959
                     /locus_tag="PLX80_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HQY20833.1"
                     /translation="MKEKNIRVIFDTNVWVSFLIGKSLSILKQYIMEGNIRIIMSQQL
                     IDELYEVTGRELKKYFPKKCVNELIDLLEIIAENVVIKPTHKLCRDVKDNFLFDLIDY
                     SKADYLVTGDKDILVHNPFLTATILSPTDFEKEMELKI"
     gene            1213..2796
                     /locus_tag="PLX80_08340"
     CDS             1213..2796
                     /locus_tag="PLX80_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQY20834.1"
                     /translation="MKFTSLSGRELDICYTPEDIKETNYYNEIGYPGEYPYTRGIHHN
                     MYRGKLWTMRQFAGFGTPEDTNQRFKYLLAHGQTGLSTAFDMPTLMGYDADDEISEGE
                     VGICGVAISSLEDMEILFDGIPLDKVSTSMTINSPAIMVYAFYLAVAEKQGVPFDKLR
                     GTLQNDILKEYIAQKEYIYPPKESMRIITDMIEYSTKEVPQFNPVSVSGYHIREAGST
                     AAQELAFTLADGFAYIEACIERGMDVDDFAPRISHFFNSHLDFFEEIAKFRAARRIYA
                     KRMKNKYGAKNPKSWTLRFHTQTAGCSLTAQQPENNIVRTAIEALAGVLGGTQSLHTN
                     SLDEALALPSDKAVKIALRTQQIIAYEHGVINVADPLGGSYYVEKLTDDIEAEAEKYF
                     DLIDAQGGVIPCIENGFFQREIADAAYRYQLELDRKEKIVVGINDFVEENEKIDIPIH
                     QISKEVEEKQVKRLKELKASRNQEEVDNALKHLRKVAEDGTNLMPAVLDCARKYVTLG
                     EMCAELKEVFGVYEEQAVF"
     gene            3004..4482
                     /locus_tag="PLX80_08345"
     CDS             3004..4482
                     /locus_tag="PLX80_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20835.1"
                     /translation="MGVPLDDTWIHFQFADNFAHGNFFEYNPGEPTAGTTSPLYVVIM
                     GLTSFVIPNFIVNSLILSGLFYILTCIFVYKISLFLFQSQYSPLKVSADSFITPEKLS
                     LLASMLTVFAGRFAWASMSGMETTIFTFFTLAGVYSHLKNLRNNNLTLLPSLMFALSA
                     VLRPEGMLIYSIYAFDVMLNLFKDKTFKQYSVKFITGLIIFLIIVLPYFIFSYYISGH
                     FFPNTFRGQGGGLSLIPNFTYLRIVVHFIFRDNFITGSLLFIFIFYYFRNIKKYFKEM
                     EYINLIALWTIFLPLISSVLIPNWRHHVRYTMPLIPFINIIAVYYLIILLNKSFLLKL
                     NSFFYSKKYAPALLVLFSFLYYIIYAAALGKNVDNINSQQVKLAEWVKNNVGRDETIG
                     INDIGAIKFISKNRIIDMAGLVTPEILQYRTYTWDDNLDSMNYLLKKNDVSYIVIYDH
                     WFKEYLERYGDQFTFITSAVLEENTICGGIEMKVYKTNFSIK"
     gene            4485..5372
                     /locus_tag="PLX80_08350"
     CDS             4485..5372
                     /locus_tag="PLX80_08350"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HQY20836.1"
                     /translation="MKIIKLIELIRPKQWIKNLFVFAPILFAAKLMDLPMLFTNIIAF
                     FAFCGISSSIYVLNDIADVESDRVHKKKRFRPLATGDVTIPQAKIMFVILIIITILLS
                     SMLPPLFIIAITLYFINNLLYTFKIKNIVLLDVFSISAGFILRVVAGAFAIDVSVSSW
                     MIITTIFISLFLGISKRRAELSGPNQENLEKQRKVLGDYDVVFVDQMNTIAATGTIIS
                     YALYTVSEKAIQSFHSEMLIYTTPFVIYGIFRYLFLLHKKNLGESPTQIVTRDIPIII
                     NSLFWLLSCALIIYKGKFL"
     gene            5377..6315
                     /locus_tag="PLX80_08355"
     CDS             5377..6315
                     /locus_tag="PLX80_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY20837.1"
                     /translation="MKPDSDILKSEKNLFIIYKAEFILLLVTLAWGLSFPLIKLTLAD
                     VSPVLFNFIRFAVTLLLFIIFFYKKIKHVKYIDLKYGLILGVFMILGFVFQTIGLQYT
                     TASKSAFITGTNLIFIPFIQFVILKSKPKYFNLISALIVLTGLFILSESFEIIPNAGD
                     ILTVICAFFFAVHIVLLDKYSAKSDVNFLIFGQFLISTVICLFFAFFYEHLILDEMKF
                     SVTPILLITLFFTVIFSTLIGIVFMTKFQRLTTPLKAGIIYNMESIFALIFAYFILNE
                     IMNSNQIIGAIIMLIGLIFSEIFGIINFKNLIGKKN"
     gene            6299..6577
                     /locus_tag="PLX80_08360"
     CDS             6299..6577
                     /locus_tag="PLX80_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQY20838.1"
                     /translation="MEKRIKITVRGFVQGIGFRFFTKRMADEFGIKGFVENMYNGDVE
                     AEAQATPDLLNDFVSELKIGPRGSAITSVFVDEIPMSDEIQDEDFIIL"
     gene            7204..8154
                     /gene="porQ"
                     /locus_tag="PLX80_08365"
     CDS             7204..8154
                     /gene="porQ"
                     /locus_tag="PLX80_08365"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HQY20839.1"
                     /translation="MLKIKALTVTLFILFFFAGNTYSQNNTYDFLRLNVGARASALGG
                     SFVTNTNDVNGLFYNPASLSTITTSQASIGFYKYLLDINSGNLAYGQKFKDEGYFGGT
                     VKYFNYGSFTKKDASANDLGTFNANDLAVTLGYSNAFQENFYYGANLKFIYSQIDEFN
                     STALAVDLGLLYVIPSTMWNFGISLLNAGTQMSQYDNTKEDLPLDLRVGVSKRLEHLP
                     VRINFEFSDLTQSGESFWENFKNLSVGGEFDFSENVKFRIGYDNAQRQDLKTGSSIGI
                     AGFSTGIGFNFLENYSFDYAFSSMGNVGTNNRIDVGFKLK"
     gene            complement(8226..9137)
                     /locus_tag="PLX80_08370"
     CDS             complement(8226..9137)
                     /locus_tag="PLX80_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQY20840.1"
                     /translation="MQKKKSSELKVGLFVFAAALIVLLSLFWAKGFSVNLSLQEYEVY
                     FSKVSGINEGDMVSLNGVRKGQVDKIELVGDSVKIMFSIDKNIRLKKDYNIYIAATEL
                     TGGKVLYIEPGKSSAEVPIGTPLQGSPGADFATLMNSFADITTDVKLLIGEFKKSTEH
                     LNGVIANVNDIVGDEGMKSSIKTTVNNLAATSQNLTQLVNESRSGINNLTSKAGNTLD
                     NIDVTIGDNGDALKNTLREIQVLTNTIDTLALNLNYVVTDINNKEKGIGKFLTDDEFF
                     NNLNATLAEIEKLSKNIRKNGVKLNIF"
     gene            complement(9140..9616)
                     /gene="greA"
                     /locus_tag="PLX80_08375"
     CDS             complement(9140..9616)
                     /gene="greA"
                     /locus_tag="PLX80_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQY20841.1"
                     /translation="MDIVYLTRSRLVEIENELMDLKSSGRKQMAEKIAEARSHGDLSE
                     NAEYDAAKEAQSHLELKIGKLETMLSRVKIISPDDMPNDEVYILSVVKVRDMKSKEEF
                     TYTLVSPEEADFELDKISVTSPIGNALLGKKKNEVVEINVPAGIIKYKVIDISKLD"
     gene            complement(9624..10898)
                     /locus_tag="PLX80_08380"
     CDS             complement(9624..10898)
                     /locus_tag="PLX80_08380"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQY20842.1"
                     /translation="MKKKSKYNLSSIDSALSDFKKGKIVIVVDDEDRENEGDMIFSAE
                     KTTPELINFLAKNGRGLICVPMEDERLKELDLNMMTSRNTSLHETAFTVSIDYLHGTT
                     TGISASDRSKTILSLVNEKTKPEDLGRPGHIFPLRSAPGGVLRRAGHTEATVDLARLS
                     GHYPAGVLCEVMKENGEMARLPDLFLIAEKFKIKIISIKDLIEFRLQREKLVTQLVES
                     SLPSEFGEFRIKLYESKVDNKEHIAIIKGKINSKEPVLVRVHSECLTGDVFGSQRCDC
                     RDQLHQSLRMIEENGSGVVLYMRQEGRGIGLHNKLKAYNLQEKGKDTVEANIELGFLP
                     DLRNYGIGAQILRDIGVRKMRLMTNNPKKIIGLNAYGLEIVERVPIEIEANTTNKNYL
                     ETKKNKLGHLLLVNSEELKKSNLKKIKRKNLN"
     gene            complement(10910..11254)
                     /gene="yajC"
                     /locus_tag="PLX80_08385"
     CDS             complement(10910..11254)
                     /gene="yajC"
                     /locus_tag="PLX80_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQY20843.1"
                     /translation="MNILKPILMQQAPGGIESILSSIVPFLLIIFIFYFLILRPQQKR
                     QKERAKLLESIKKGDKIITAGGMHGLVEGLDDKTLLVKITDNVKVKMERSAVTTIVGV
                     TDLEPEKKSLLG"
     gene            complement(11300..12451)
                     /gene="tgt"
                     /locus_tag="PLX80_08390"
     CDS             complement(11300..12451)
                     /gene="tgt"
                     /locus_tag="PLX80_08390"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQY20844.1"
                     /translation="MNKDFFKVLSHSNEAGVKARTGIIKTESSEIETPVFMPVGTLGN
                     VKALENRELLEFDTRVILANTYHLFLRPGMEVMENADGLHKFMNWDRTLLTDSGGFQV
                     FSLAKLRKISEDGVEFSSHLNGDKHFFTPEKVIEIQRSIGSDIMMPLDECLPYPSDHE
                     KVKKSIALTYRWEKRCYEHLQNTESLYGRKQFLFSINQGSAYKDLRKESIQMLSDIDF
                     DGNAIGGLAVGEENSLMYEITDLCTDHLAKEKPRYLMGVGTPEDLLECVERGVDMFDC
                     VMPTRNARHGRLFTTHGEINLKSAKLKFNNSSPDEEFRSYTSENFSLAYLRHLFISNE
                     IMGFQLATIHNIGFYLNLMKNIRSAVRENKFLKFKKDFLEKYKSNSTFK"
     gene            complement(12510..13259)
                     /locus_tag="PLX80_08395"
     CDS             complement(12510..13259)
                     /locus_tag="PLX80_08395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20845.1"
                     /translation="MPDNSLSVKKISFIIPALNEEKLLRRTLLQFTENLKKDNDLEII
                     VSDGGSTDKTSEIANELADKVIFKNENIPQNISRGRNAGAKHSQGDVLVFLNADTYVK
                     DIELMLKKINEEFTDQNLAAIACKIKVFPDEEIVSDKMFHTFYNNYVRLLNKFFMGMG
                     RGECHIVSRKKFFEVNGYNEEMAAGEDFDLYLRLSKTGKIIFNNELIVYESPRRYRKY
                     GYINVFWNWTINSISVFLFKKSVSKSWEPIR"
     gene            complement(13252..13464)
                     /gene="rpmB"
                     /locus_tag="PLX80_08400"
     CDS             complement(13252..13464)
                     /gene="rpmB"
                     /locus_tag="PLX80_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQY20846.1"
                     /translation="MSKICAVTGKKPLVGNNVSHANNKTKRRQLPNLQKKRIWLEEEK
                     KWVTLKISAKGIKTLNKKGTQILLNA"
BASE COUNT         4236 a         2396 c         2096 g         4836 t
ORIGIN      
        1 tgagaaatgt acttgattta tttgttaata gatgttggtg ttttcaaaaa ctttgcctga
       61 ggttcagttg aaaattacta taaatcttaa gcaaactttt taatcaccat caacaaaagg
      121 agacttgact ccagcagctg gaaaaaatag gctgaaataa aatgcgcttc tcctcatcta
      181 tatttatatc tataaaaaat aaatcacttg taaaacatta tttacagatt tatttttaat
      241 tcggtaaaat attctttatg ataaacactt tttgaataca cggatcacgg caaattataa
      301 ttattcaaat aaaaatttaa ataatatgaa aacagaaaat atatctaatt tgacttttga
      361 tcggctactt gagttggtca gacaattatc atttcaggaa aaaataatat tatcaaagga
      421 gttagaaaaa gaatgtattg attctaaatt gtcaagttta cttaaaacat ttaaaacaaa
      481 tgaattgagt gaagatacta ttaacgaaga agttgaatat gtaagagatc aaatgtatga
      541 aagaaaaaaa cattagagtt atttttgata caaatgtatg ggtaagtttc ctgattggaa
      601 agagtctttc aattttaaaa caatatatta tggaaggtaa tattagaatt attatgtccc
      661 aacaattaat tgatgagtta tatgaagtta cagggagaga actgaaaaaa tattttccaa
      721 aaaaatgtgt aaatgagctg attgatctcc tggagataat tgcggagaat gttgttataa
      781 aacccacaca taaactttgc agagatgtta aagataattt tctatttgat ctgatagatt
      841 attcaaaagc agattattta gtaactggtg ataaagatat tttagtgcat aatcctttcc
      901 tgactgcaac gattttatct ccgaccgact ttgaaaaaga gatggaatta aaaatttaat
      961 aatttcattg cgtattattt tcacgactga atttctaatt tttgataaac ctttgacgac
     1021 ttaaaaattt cttaatccca ctttctttca atccatttca tttctgaatt acatttcccc
     1081 cataaaaacc atatatttgt aaccatattt tattaatact taactacgat tatgaaaaat
     1141 tactccgagc caaagaagag cgcttccaaa tccgaatgga aagaagatgc tctgaaatca
     1201 caagccagcg gtatgaaatt cacttcactc agcgggcgcg aactcgatat ctgctacact
     1261 cctgaagaca ttaaagaaac aaactattat aatgagatcg gctatccggg agaatatccc
     1321 tacacccgcg gtatccatca caatatgtac agaggtaaac tctggactat gagacagttc
     1381 gcaggattcg gaactcccga agataccaat cagagattta aatacctgct tgcgcacggt
     1441 cagacaggat tatcaacagc attcgatatg cccacactta tgggatatga tgctgatgat
     1501 gaaatttccg aaggcgaagt cggtatctgc ggtgttgcga tttcatcttt agaggatatg
     1561 gaaattctct ttgacggaat tcctcttgat aaagtctcta catctatgac tataaactcc
     1621 cctgccatta tggtctatgc attttatctt gcagtcgccg agaagcaggg cgttccattt
     1681 gataaattaa gaggtacact tcagaacgac attttaaaag aatatattgc tcagaaagaa
     1741 tatatctatc ctccgaaaga atccatgagg ataattactg atatgataga atattcgact
     1801 aaggaagttc cgcagtttaa tcccgtcagt gtcagcggtt accacatcag ggaagccgga
     1861 tctactgctg cacaggagct tgcattcaca cttgcagacg gattcgcata catcgaagct
     1921 tgtatagaaa gaggaatgga tgttgatgat tttgcaccaa ggatctcaca tttctttaat
     1981 tcgcatttag atttttttga agagatagca aagttcagag cagcacgaag gatctacgcc
     2041 aagagaatga aaaataaata cggcgcgaaa aatcctaagt catggacttt aagatttcat
     2101 acacagactg cgggatgttc actcactgct cagcagccgg aaaataatat tgtaaggact
     2161 gccattgaag cccttgcagg tgtactcggc ggcacgcaga gtcttcacac aaattccctg
     2221 gatgaagctc ttgcacttcc atctgataaa gcagtaaaga tcgcattaag gacacagcag
     2281 atcattgctt atgaacacgg tgtaataaat gtggccgatc cattgggcgg aagttattat
     2341 gtagaaaaac tcactgatga tattgaagca gaagctgaaa aatattttga tctgatcgat
     2401 gcgcagggcg gtgtgattcc ctgtattgaa aacggattct tccagagaga aatagctgat
     2461 gcagcttaca gatatcagct tgagcttgac aggaaggaaa agatcgtagt cggcataaat
     2521 gattttgttg aagaaaatga aaagatagat attcctattc atcagatctc aaaagaagta
     2581 gaagaaaaac aggttaagag acttaaagaa ttgaaagctt caaggaatca ggaagaagtg
     2641 gataatgcgc tgaaacatct tcgaaaagtt gcagaggacg gaactaacct aatgcctgct
     2701 gtacttgact gcgcaaggaa atatgttaca cttggagaga tgtgtgcaga gctgaaagag
     2761 gtttttggtg tttatgaaga acaggcagtt ttttaggtct aaaaattgtc agttacaagt
     2821 gatctgatac agattcgaat ttaaatttat aaattttgag cgggataatt ctcgctcatt
     2881 ttattattac tgaattttac tataatatct attaaagata aaaactacat atacataata
     2941 tctgcctgcc tgatttcaat tataattttt ttatcgtttg aattttactt cacggatgga
     3001 aaaatgggtg taccgcttga tgacacatgg atccattttc agtttgctga taattttgct
     3061 cacggaaatt tctttgagta taaccccggc gagcctacag caggtaccac atctccttta
     3121 tatgtggtaa tcatgggatt aactagtttt gtaattccca attttattgt taattctctg
     3181 atcctttcgg gattgttcta tattctaaca tgtatttttg tttataagat ttctcttttc
     3241 ctttttcaaa gtcaatattc tcctttaaaa gtaagtgccg attcatttat caccccggaa
     3301 aaactttctc ttttagcatc catgcttaca gtttttgccg gccgatttgc ctgggcttct
     3361 atgtcaggta tggaaacgac tatatttaca ttttttacat tggcaggtgt ttattctcat
     3421 ttgaagaatt tacgaaataa taatctgact ttacttccgt ctctgatgtt tgctctttct
     3481 gctgttctca gaccggaagg tatgctgata tattcaatat atgctttcga tgttatgctt
     3541 aacctgttta aagataagac gtttaagcaa tattcggtaa agtttattac aggactgata
     3601 atatttctta tcattgtcct tccatacttt atcttttcgt attacataag cggacatttt
     3661 tttccaaaca ctttcagagg tcagggcgga ggtttaagtc ttattccaaa tttcacttac
     3721 ctcagaattg ttgttcactt tattttcagg gataatttta taacaggttc tttactattc
     3781 atatttatat tttactattt cagaaatata aaaaaatact tcaaagaaat ggaatatata
     3841 aatctgattg cactctggac aatatttctt ccgctcattt catctgtgct gatcccaaac
     3901 tggcgtcatc atgtaagata tacaatgccg ctcattccat ttataaacat catcgcagtt
     3961 tattatctga tcattttatt gaataaaagt tttctgctta aattgaacag ctttttttat
     4021 tcaaaaaaat atgcacctgc attgctagtt ttgttttcat ttctctacta tattatatat
     4081 gcagcagctt taggaaagaa tgttgataat ataaattctc agcaggtaaa acttgctgaa
     4141 tgggtaaaaa ataatgtagg aagggatgaa actataggta ttaatgatat tggtgccatt
     4201 aaatttatca gtaaaaacag gattatcgat atggcaggac tggttactcc tgagattctt
     4261 caatacagaa cttatacatg ggacgataac ttagactcaa tgaattatct attaaaaaaa
     4321 aatgatgttt cctatattgt catctatgat cactggttta aagaatatct tgaaagatat
     4381 ggtgatcagt ttacatttat aaccagtgct gtacttgaag aaaatacaat ctgcggcgga
     4441 atagaaatga aagtgtataa aactaatttc agtataaaat aaatttgaaa attataaaac
     4501 tgatagaatt aatcagacca aagcaatgga tcaaaaatct gtttgtcttt gctcctatat
     4561 tgtttgcggc aaaacttatg gatctgccaa tgctgtttac aaacatcatt gcattttttg
     4621 ctttctgcgg aatatcaagc agtatatatg ttttaaatga tattgccgat gtagaatctg
     4681 acagagtgca taagaaaaaa agattcagac ctcttgctac gggagatgtt acaattccgc
     4741 aggcaaaaat aatgtttgta attctgatca ttataacaat tttactgagc agtatgctgc
     4801 ctcccctttt cataattgct attactctat attttattaa taatcttctt tacactttta
     4861 aaataaaaaa tattgtactg ctcgatgtat tttcaatctc agcaggtttc attctcagag
     4921 ttgtcgcagg tgcttttgca attgatgtca gcgtttcaag ctggatgatc attactacta
     4981 ttttcatttc actgttcctt ggaatttcca aaagaagagc cgagctctcc ggtcctaatc
     5041 aggaaaatct cgaaaagcag cgaaaagtat taggtgacta tgacgttgta tttgtagatc
     5101 agatgaatac tattgcagcc accggtacga tcatttctta tgctctctac acagtttctg
     5161 aaaaagcaat acagtctttt cattctgaaa tgcttatcta tactacgcca tttgtgatct
     5221 atggaatttt cagatatcta ttccttcttc acaaaaagaa ccttggagaa agtcctactc
     5281 agatcgtaac aagagacatt ccgatcatta taaattctct gttctggctg ctctcctgcg
     5341 cgttgattat ttacaaaggg aaatttttat aattgagtga aacctgactc cgacattcta
     5401 aaatccgaga agaatttatt tattatttac aaagctgaat ttatcctgct gcttgtcact
     5461 ttagcatggg gactttcatt tccattaata aaactaacac ttgcagatgt ctcgcctgtt
     5521 ttatttaatt ttatcaggtt cgctgttact ttacttcttt ttataatttt tttttataaa
     5581 aagataaagc atgtaaaata tattgatcta aaatatgggt tgatacttgg tgtatttatg
     5641 atccttggat ttgtctttca gaccattgga ctgcaatata caacagcttc caaatcagct
     5701 tttattaccg gcacaaatct gatcttcatt ccatttatcc agtttgttat tttaaaatca
     5761 aagcccaaat attttaatct catcagtgct ctgatcgtat tgaccggtct ctttattctt
     5821 tccgaatcat ttgagatcat tccgaatgct ggtgacattc ttactgtaat ctgtgcattt
     5881 ttctttgctg tccatatagt tttactggat aaatattcgg caaagtcaga tgtgaatttt
     5941 ttgatattcg gacaattcct tatctcaacg gttatttgtt tattctttgc tttcttttat
     6001 gaacacctga ttcttgatga aatgaaattt tcggtcacac caattttatt aataacatta
     6061 tttttcacag tgatattttc tactttaata ggtattgttt ttatgacaaa gtttcagcgt
     6121 ttaactacac cactcaaagc cggaatcatt tataatatgg aatcaatatt tgctttaata
     6181 tttgcatact tcattttaaa tgaaatcatg aattcaaatc aaattatagg tgcaataata
     6241 atgttgatcg gattaatttt ttcagagata ttcggtatta taaattttaa aaatttaatt
     6301 ggaaaaaaga attaaaataa ctgtaagagg tttcgtccag ggaattggct ttagattttt
     6361 tacaaaaaga atggcagatg aattcggcat caaaggtttt gtagaaaata tgtataacgg
     6421 cgatgtcgaa gctgaagctc aggctacacc ggatctgtta aatgactttg tatctgaatt
     6481 aaagatcggt ccccgcggct ctgcaattac ttccgtattc gtagatgaaa tcccaatgtc
     6541 agatgaaata caggatgaag attttattat tttataatct cctgttttac gaatcatctg
     6601 gatacccatt ttttttcagg aataattttt tattaacgtt agaaaaaact aagcagaaaa
     6661 aagtgaatac tgaattatgt aattaaaaaa ttttggatta ctgtaaaatt attctttttt
     6721 ttaaaataga tataggacac ctgatcagca aaccaactat cagtgcttat aggagtttga
     6781 agacatattt tctcatttat tgaagaagtg cggataacag gaaaatttta atattttata
     6841 atctcaaaaa tttcaaataa atccgaacta taaccattac aatcgtgttg tgaaacccat
     6901 gcaatatcaa cagcatctat aatcatccaa taatgaaacg ataatttgaa attactttta
     6961 atacgtaata tattttttta tttaaattta acctccggtt taattttgtc agctttctta
     7021 aaaagtaagt aaaaaaatag atcattaatt ttatacttta actttccaga cagaattaat
     7081 taaaagtttt aaatcacgtc aaatttgcaa ttgacaataa acatttaaac taatattatt
     7141 gtgaaactta aattgaacct atgacgagac aattgttact tattctttaa caaattaaga
     7201 aaaatgctga aaatcaaagc tttaacagtc accttattta tacttttctt ttttgccggt
     7261 aatacttact cacaaaataa tacttatgat tttcttagat taaatgtagg agctcgtgct
     7321 tctgcactgg ggggaagttt tgtcacaaat acaaatgatg taaatggttt gttttataat
     7381 cctgcatcac tttcgactat tacgacttca caggcttcta taggatttta taaatactta
     7441 cttgatataa attccggtaa tcttgcctat ggtcagaaat ttaaagatga gggttatttc
     7501 ggcggtacgg taaaatattt taattacggc agctttacta aaaaagacgc atcggctaat
     7561 gatctcggaa catttaatgc aaatgatctt gctgttactt taggttattc aaatgcattt
     7621 caggaaaatt tttattacgg tgccaatctg aaatttattt attcgcagat cgatgaattc
     7681 aattctactg ctcttgctgt cgatcttggc ttgctttatg taattccttc tacaatgtgg
     7741 aattttggta ttagcttatt aaatgccggt acacagatga gtcagtatga taatacaaaa
     7801 gaagatcttc ctctcgatct tagagtcggt gtcagtaaaa gacttgagca tctgccggta
     7861 aggatcaatt ttgaattcag tgatcttact caaagcggtg aatcattctg ggagaatttc
     7921 aagaacttat ctgtcggcgg tgaatttgat ttcagcgaga atgtaaaatt cagaattgga
     7981 tatgataatg ctcagagaca ggatcttaag acaggaagtt cgatcggtat agcaggattt
     8041 tctacaggaa ttggatttaa tttcttagag aattattctt ttgattatgc tttcagttcg
     8101 atgggaaatg tcggaacaaa taacaggatt gatgttggat ttaaattgaa ataaagttta
     8161 atatataaat taagaaagcc gggcaatcca aattgtccgg ctttttttat gttaaaatta
     8221 taagatcaaa aaatatttaa cttaactcca ttcttcctga tattcttaga aagcttttct
     8281 atttctgcaa gagttgcatt cagattatta aaaaattcat catcagtcaa aaattttcca
     8341 atcccttttt ctttattatt gatatccgtt actacataat ttagatttaa agcaagcgta
     8401 tcaatagtat ttgtcagcac ctgaatttct ctcaaagtat ttttcaaagc atcaccgtta
     8461 tctccaattg ttacatcaat attatcaaga gtattgcccg ctttgcttgt aagattattt
     8521 attccgctgc ggctttcatt tacaagctga gtcagattct gagaagtcgc tgcaagatta
     8581 tttacagttg tctttattga gctcttcatc ccctcatcac ctactatatc atttacattc
     8641 gcaatcactc cgttcagatg ctcagtggat tttttgaatt ctccaataag cagtttcaca
     8701 tcggtcgtaa tatcagcaaa cgaattcatg agagttgcaa aatctgcacc aggacttccc
     8761 tgtaaaggag ttccaatcgg tacttctgca gagcttttcc ccggttcaat atacaaaacc
     8821 ttaccgccgg ttaattctgt cgctgcaata tatatgttgt aatctttttt taatctgata
     8881 tttttatcaa tactaaacat gatctttaca gaatcaccaa ccaattcaat cttatctacc
     8941 tgacctttac ggactccgtt taagcttacc atatcacctt cattgatacc gctgactttt
     9001 gaaaaataaa cttcatattc ctgtaaagat aaatttacag aaaatccttt tgcccagaat
     9061 aaagagagca aaactattaa tgctgctgcg aaaacaaata acccgacttt taattctgat
     9121 gattttttct tttgcataat taatctaatt tagaaatgtc aattacttta tattttatga
     9181 tacctgccgg tacattgatc tctaccactt cattcttttt cttaccaagc aaagcattac
     9241 ctataggtga tgtgacagaa attttatcaa gttcaaagtc cgcttcttcc ggagagacaa
     9301 gagtatatgt aaattcttct tttgatttca tatcacggac tttcacaact gacaaaatat
     9361 aaacttcatc atttggcata tcatccggtg aaataatttt aactctcgaa agcattgtct
     9421 caagttttcc tatctttaat tcaagatgac tctgcgcttc ttttgcggca tcatactcag
     9481 cattctcgct cagatcaccg tgagatcttg cctctgcaat tttttcagcc atttgttttc
     9541 tgccgctgga tttcaaatcc attaactcat tttcaatttc tactaatctg cttcttgtta
     9601 aatatacgat atccattttt tctttagttt aaattttttc ttttaatttt ttttaaatta
     9661 ctttttttta attcttcaga attaacaaga agtaaatgtc ctaacttatt tttttttgtc
     9721 tcaagataat tcttgttagt ggtgtttgct tctatttcaa tcggaactct ttccactatc
     9781 tcaagaccat atgcatttaa ccctataatt tttttgggat tatttgtcat aagtctcatc
     9841 ttccttacac caatatctct gagtatctgt gcaccaatac cataatttct cagatcagga
     9901 aggaatccaa gttcgatatt cgcttcgact gtatctttac ctttttcctg aagattgtat
     9961 gctttaagtt tattgtgaag tccaattccc ctgccttcct gtctcatgta taaaacaacg
    10021 cctgagccat tctcctctat cattcttaat gactggtgaa gctggtctct gcagtcgcat
    10081 ctttgtgagc caaatacatc tccggtcaga cattccgaat gaactcttac aagtaccggt
    10141 tctttagaat ttatttttcc ttttataatt gcaatgtgct ctttgttatc tactttactt
    10201 tcatacagtt ttatccggaa ttctccaaat tcacttggca gactggattc aactaactgt
    10261 gtaactaatt tctcacgctg aagtctgaat tcaatcagat cttttataga aattattttg
    10321 atcttaaatt tttctgcgat caaaaacaga tccggaagtc ttgccatttc accattctct
    10381 ttcatcactt cacataaaac tcctgccgga taatgtcctg ataatctcgc aagatcaact
    10441 gtagcttctg tatgccctgc ccttcttaaa actccgccgg gtgcggatct taaagggaaa
    10501 atatgtccgg gtcttcccag atcttcgggc tttgtcttct catttactaa tgacaatatt
    10561 gttttacttc tgtcggaagc agaaataccg gttgtcgtac catgcaaata atctatgctg
    10621 actgtaaaag ctgtttcatg cagtgatgta tttctggaag tcatcatgtt aagatccaac
    10681 tctttcaatc tttcatcttc cattggaacg cagatcaatc ctcttccatt ctttgcaaga
    10741 aaattaatca gttcaggtgt agtcttctca gctgagaata tcatgtcacc ttcattttcc
    10801 ctgtcttcat catcgaccac tattacgatc tttccttttt taaaatcact taaagcagaa
    10861 tcgatcgaac ttaaattata ttttgatttc tttttcaatt ataaaaattt taacctaata
    10921 aagatttttt ctccggctca agatctgtaa ctccgactat tgtggtcact gctgatcttt
    10981 ccatcttcac ttttacgtta tctgtaatct tcaccagcaa tgtcttgtca tcaagtccct
    11041 caaccaatcc atgcataccg cctgcggtta tgatcttatc tccttttttg attgactcca
    11101 gaagttttgc tctttctttt tgtctcttct gctgaggtct tagaattaaa aaataaaata
    11161 taaaaattat aagaagaaat ggtactatgg aagataatat agattcaatt cctccgggag
    11221 cttgctgcat caatataggt tttaaaatgt tcattttcgt tattattatt ttaagttttt
    11281 attaattatt tattaatttt tatttaaaag ttgaattact tttatatttt tctaaaaaat
    11341 ctttcttgaa ttttaaaaat ttattctctc tgactgctga cctgatattt ttcataagat
    11401 tcaaataaaa tccaatatta tgaattgtcg ccagttgaaa tcccattatc tcatttgaaa
    11461 taaacaaatg tctcaaatat gccagtgaaa aattctcaga tgtatatgat ctgaattcct
    11521 catcaggtga actattgtta aatttaagtt tagcactttt taaatttatt tcaccatgtg
    11581 ttgtaaaaag tcttccgtgt cttgcattgc gtgtaggcat tacacagtca aacatgtcaa
    11641 ctcccctttc aacacattcc agaagatctt ccggcgtccc tactcccatt aaatatctcg
    11701 gcttttcctt agccaaatga tcagtacaaa gatctgtaat ttcatacatt aaagaatttt
    11761 cttcacctac tgcaagtcca ccgatcgcat ttccgtcaaa atcaatatcg gatagcattt
    11821 gaatactttc ctttctgaga tctttgtaag cacttccctg atttatagaa aataaaaatt
    11881 gctttctacc gtaaagactt tctgtatttt gcaaatgttc ataacacctt ttttcccacc
    11941 tatatgtaag agcaattgat ttctttactt tttcatgatc acttggataa ggtaaacatt
    12001 catcaagagg catcataata tctgacccga tcgatctctg aatttcaatt actttctcag
    12061 gagtaaaaaa atgtttatct ccatttaagt gtgatgaaaa ttctactcca tcttcagaga
    12121 tcttcctgag cttagcaaga ctaaacacct gaaatccacc gctgtctgtt aatagtgtcc
    12181 tgtcccagtt cataaattta tgaagtccgt ctgcattttc cataacttcc attccgggtc
    12241 tcagaaataa atgataagtg tttgcaagaa taactcttgt atcaaactcg agcagctccc
    12301 tgttttcaag ggctttgaca tttccaagcg ttccgacagg cataaagaca ggagtttcaa
    12361 tttctgaact ttcggttttg attattcctg ttctggcttt tacacctgct tcattactat
    12421 gtgaaagaac tttaaaaaaa tctttattca ttttttttgc cggcagatat aattcttaaa
    12481 cctgataaat atatttaatt ctgaggaatt tatctgatcg gttcccatga ttttgaaaca
    12541 gattttttaa ataaaaaaac tgagatcgaa tttattgtcc agttccagaa tacatttata
    12601 tatccatatt ttctatacct tcttggtgat tcatatacga tcaattcatt attaaaaata
    12661 attttaccgg ttttacttaa tcttagataa agatcgaaat cttctccggc agccatctct
    12721 tcattatatc cattcacttc aaaaaatttc tttctggaaa caatatgaca ttcacctctg
    12781 cccataccca tgaagaattt attcaataat cttacataat tattatagaa tgtatgaaac
    12841 attttatctg aaacaatctc ttcatctgga aatactttta tcttacatgc aattgctgcc
    12901 agattttgat cggtaaattc ttcattgatc ttcttcagca taagttcaat atcctttaca
    12961 taagtatcag catttaaaaa aacgagaaca tctccctgag aatgctttgc acctgcattc
    13021 cttccccggg agatgttctg cggaatattt tcatttttaa agataacctt gtcagctaat
    13081 tcatttgcaa tttcagaggt tttatctgta ctccctccgt cactgacaat aatctccaga
    13141 tcattatctt tttttaaatt ttctgtgaac tgtaataatg ttctccttaa taatttctct
    13201 tcattaagag caggtataat aaagcttatc ttttttacgg ataaactatt atcaggcatt
    13261 caataaaatc tgagtacctt ttttattcaa ggtttttatt ccttttgctg aaattttcaa
    13321 tgttacccat ttcttttctt cttcaagcca gatccttttt ttctgtagat taggaagctg
    13381 tcttcttttt gttttattat ttgcatgaga aacgttattt cccaccagcg gtttttttcc
    13441 ggtaactgca caaatttttg acatgatatt ttccttacta tggtttatat ggtttataag
    13501 tggattcgta aactttcata tttctgtcaa gaaatttgaa tgttcctttt tccgtcttga
    13561 ttgt
//
LOCUS       DAORVQ010000088        13388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2159_length_13388_cov_5.372009, whole genome shotgun sequence.
ACCESSION   DAORVQ010000088 DAORVQ010000000
VERSION     DAORVQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_13388_cov_5.372009"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..749
                     /locus_tag="PLX80_08405"
     CDS             348..749
                     /locus_tag="PLX80_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQY20847.1"
                     /translation="MIRISKKVEYALMALKFISESDNRLVTAREISDSKKIPYDLLSK
                     ILQKLKNEEILLSNQGSNGGYSMNKRTDEIYLYNLMSMLDGEIAIADCLAESKDKDCC
                     LEDSCSIKSPITKLQKEIENLFKNKTISDFV"
     gene            771..1943
                     /locus_tag="PLX80_08410"
     CDS             771..1943
                     /locus_tag="PLX80_08410"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HQY20848.1"
                     /translation="MKFPVYMDYNATTPVDPEVLEEMIPYFCEHFGNASSANHKFGWN
                     AEEAVSKARKNIADLINASPREIIFTAGSTEANNISLKGVAEIYEGKGNHIITCTSEH
                     KAILETCEYLETRGFEVTYLKVDKDGLIDLDELKNAITDKTILVSIMAANNEIGTIQP
                     VEEIGKICRENGVLFHTDATQAVGKIPVDVQKMNIDLMSISAHKFYGPKGVGALYVRS
                     KEPRVNFTKQMHGGSQEKGLRGGTLNVPGIVGMGKACEISMKNMNNEMSKHIVFRDRI
                     IDALLNINDVYLNGHRTNRLTNNINFSIGKINIESLLGDLKDIALSTGSACTSSSMAP
                     SHVLSAIGREDGLARSSIRISFGRFTTEEEIDYLIEKLTSNILKLREEAPEFQIAK"
     gene            1986..2351
                     /locus_tag="PLX80_08415"
     CDS             1986..2351
                     /locus_tag="PLX80_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQY20849.1"
                     /translation="MTEVKEFIPGVSEDVNITDKAIKEVKKIMEENKVPENYGLRVGV
                     KGGGCSGMTYSLGFDPDERTGDTVLEKDGIKIFIDMKSVLYLSGTEIDFTDGLTGRGF
                     EFNNPQAVKTCGCGSSFGV"
     gene            2589..3218
                     /locus_tag="PLX80_08420"
     CDS             2589..3218
                     /locus_tag="PLX80_08420"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQY20850.1"
                     /translation="MAYEWKFNPRPYTDDEASELLAEVIDADTANWHYNTHHKGYVTF
                     MNKIETELETADRASANGNYSNVGELKRRFTWNHAGALLHDVYWEVLGGDGDTSKGPE
                     VVAAIEKEFGSIDNWKADFKATAVSAKLSGWAVLTYDQLYSERLLDILVDEHHYGAIW
                     GGIPIICCDVFEHAYYHKDGPGRATYIDNFFKNLNWSRINDRYKKYAVK"
     gene            3294..3851
                     /locus_tag="PLX80_08425"
     CDS             3294..3851
                     /locus_tag="PLX80_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20851.1"
                     /translation="MKRFFAFTIFIFILSSVFISCDKTDTEQTEINNKVFSWRPDLKI
                     TDIPDTPVKGFLKGKEIEFTYINFEDWRGSGDNVLNFSNLIPKNSCGFIENDNSFQLM
                     KKAGEIKVGELIKSSFDQNLDGFTAYYEIADSTNPVKSTVEWNCILVITAKNEKTIKG
                     KIAMCFKDDTKSWIAGTFEAIRCYN"
     gene            3878..4393
                     /locus_tag="PLX80_08430"
     CDS             3878..4393
                     /locus_tag="PLX80_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20852.1"
                     /translation="MKKIILSVFFISALILTGCDNKEKEAVKESNETEKEMTEKSENQ
                     IKEELNKDESENSEDQSKIENELGMKPGLPADFPKEIPHPKNAKVLGSLNSSDGTVVT
                     FESNDKVLDIVEFYKEEMKKGGYTLTEEGESLVSDKGGLINWSKGNKIAGLMLGFDKD
                     KNITSLVITYN"
     gene            4469..5113
                     /locus_tag="PLX80_08435"
     CDS             4469..5113
                     /locus_tag="PLX80_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQY20853.1"
                     /translation="MDEFTDLPLFPLNVVMFPHAKMELHIFEERYKKMINECIDEGKI
                     FGINLFANEKIFLTGCTSEISEVKRKTITGEFDIVSKGIRRYEIMNYKMTANKYFVGE
                     VIFSKNSNFITDDIKFHEAVNNYNELTDIVYKGSIKPIDPNIEKWQTGEFSPSFLMAE
                     KCGLSVAERQTLLEINNEDDRIFFMLNYFESIMPKLKDADRIAKIIMSDGYLVQ"
     gene            5131..6126
                     /locus_tag="PLX80_08440"
     CDS             5131..6126
                     /locus_tag="PLX80_08440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20854.1"
                     /translation="MIEKDKVKNILVTRTDRLGDVILTLPLISCLKKIFPESKIFFLV
                     KVYLKDILDCYEGIDEIIYINNEEDKDILSEIIRSKNIDLAINVKPDYKLALMLYRLG
                     IRYRIGTAYRWFSFLYNCKVHVHRKRSDLHESELNLLMIKSFFDEECSSEFGKFKIEE
                     SKKLRLDQKLSEFNLELSDTYIIIHPGSGGSAKDLPAENFIDFINMFSSEYRNIKIVF
                     TGLQSENELAEKLINGSGIEKSVVNLCGKLDLGEMLILINECSLFISNSTGPIHIAGA
                     LNKKIIGFYPNEKPMHAGRWKPLGKDVIIISPESSIDDLSEIDTNEIINAVKKLI"
     gene            6206..7018
                     /locus_tag="PLX80_08445"
     CDS             6206..7018
                     /locus_tag="PLX80_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQY20855.1"
                     /translation="MKIIRSVILITLLFTSVFLNAQDNEFRTIEQDAFTTGEKLTFEI
                     NYGFVNAGTAVMEIDPNLQTINGRECYDIRFTVNSSGSFDWVYKVRDFYRTYVDKEGL
                     FPWRFEQHIKEGNYQRDFEAIFDQENHKAKTYTGEKEPKKFEGEFDIPEYTQDAMSAF
                     YFFRTLDFSKMKEGDETLLQNFYKDNTYPLKVKYYGKETIEVPAGEFRCVKLEPLVAE
                     GGLFKSEGSIIVWLTDDERRMPVKVKTKVVIGSIDADLSAYSGLAGSLNSKK"
     gene            7073..7804
                     /locus_tag="PLX80_08450"
     CDS             7073..7804
                     /locus_tag="PLX80_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQY20856.1"
                     /translation="MKISELFYSIQGEGKRAGRPSFFIRTNHCNLRCKFPGGNLCDTY
                     YTSWEPDDLRNLGELNLDDIIAEYKKIKCCDIVITGGEPTMNANDLSDLCQKLKESNP
                     DSFITIETNGTYFGDFIKYSELISVSPKLKSSVPLGTEYEKMHEKNRLNFETFIKFNL
                     LKENYLKDIQWKFVFTSEKDIKEIREIQSAVGFKDSDIYLMPEGITAEDLNSKRLETL
                     EVCKKNNFNYSDRLHILAWGNKRGV"
     gene            complement(7930..8778)
                     /locus_tag="PLX80_08455"
     CDS             complement(7930..8778)
                     /locus_tag="PLX80_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQY20857.1"
                     /translation="MRRISFILILILFFYVNSFSQYRYKTSGKDKTKYSEKSIDVKLF
                     RTFNNIHSSFINSMVNITNESIIPISIAAPVGMYTFARINNNSYDESSAILLALSEIT
                     NLSVTQIIKVTVKRPRPFRTMNNVYLSDTLSVKGTYSFPSGHSSEAFAIATSLTLRYP
                     VEPVLIAGLYTYAAVVSLGRIYWGVHYPSDVFTGMLIGAGSAALIYSLRAPIIKAKND
                     LFNQSERTDSYSPGVAPASMFIAIAAADVINHFFRNSNNTLLQKSNVFFDVNKDAGRI
                     NYSVGF"
     gene            complement(8782..9921)
                     /locus_tag="PLX80_08460"
     CDS             complement(8782..9921)
                     /locus_tag="PLX80_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HQY20858.1"
                     /translation="MKFATKQIHAGQHPEETTGAVITPIFQTSTYSNKKLGDSKGFDY
                     GRTINPTRSTLEANLAALENGKYGFCFSSGMAAVQTVINLFKPGDHVICTNNVYGGTY
                     RLYEQVMKNYGLEFSYVDTSDNEALINAIKPNTKFIYVETPTNPMLNITDLKNLGEIG
                     REKKITTCVDNTFMSPYFQNPLDFGIDIVLHSTTKYINGHSDVIGGCLVTSDETIAET
                     FKFLQNSIGSVPAPFDCWLILRSTKTLAQRMKAHNENAVTICKVLSENSMIEKLYYPG
                     LESHPQHELAKSQMSGFGGIISLDVGSYEKAVDFCNALEIFQIAESLGGVESLVCHPV
                     SMTHGTVPKYLREEFGLTDGLVRLSVGIEDIEDLLADINNALSKL"
     gene            complement(10012..11370)
                     /locus_tag="PLX80_08465"
     CDS             complement(10012..11370)
                     /locus_tag="PLX80_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQY20859.1"
                     /translation="MKYYNSVLDLIGNTPLVKLNNITKGLKPTVLAKMESYNPGGSVK
                     DRIGLNMIEAAEREGKLKPGGTIIEATSGNTGIGIALVAALKGYKTIFVMTEKASREK
                     NNYLKALGAEVIIQPMTAKHGEPDHYVTVAERLSKEIPNSLFLYQYVNPGNPEAHYKT
                     TGPEIWNDTEGKITHFVSGVGTGGTISGTGKYLKEKNPGIKVIGADPYGSIFKVVKET
                     GNTPEPIPYLIEGIGQDVMVENVQLQYIDEIVNVNDLDSVEMCKRLGKEEGIFCGGST
                     GTIAYAAVELAKTLDENAVIVFIVCDTGERYLSKYHSDEWLREKRLMNSDFISTGQVF
                     LTKKTAGVPGLISAKSSDKLFEALELMDKYNLSTLPVIDGSDCVGSIAEESVIEKVMN
                     DRNVSGKQVKDVMDKKLPIVDFNTEFPKALEMLQKDSTILVSQHGQIKGILTRYDVLD
                     FV"
     gene            complement(11437..12282)
                     /locus_tag="PLX80_08470"
     CDS             complement(11437..12282)
                     /locus_tag="PLX80_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20860.1"
                     /translation="MTTLNLKHILPYIFILSTVIFFSNESLYSQKKTESVKTDEFILI
                     TDNQGNILDGDKKDWTYNDKSYNKINAIENLFVNPVNDTNLKNQPDWKIVDEIIGNKL
                     LLKWNTPKGFTCKGFAVERIEIKPDSSESDWVEIDYVRGVKTAPTTSKYSYIDKKLKE
                     GKYKFRIKMDMNDGTSKYLNLNKEVFWMEFPTTLQFYPVYPNPVKEKFNVRFFLPSND
                     EISIFFINGTDTVYVMKKIKLGQGFYEKSIDKNTFKYYNEVKRIYLTRKSMRRKEYTG
                     DIKFE"
     gene            complement(12456..13292)
                     /locus_tag="PLX80_08475"
     CDS             complement(12456..13292)
                     /locus_tag="PLX80_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20861.1"
                     /translation="MNRQILRHEIYIIIFSAVIFFSNKNVYSQNEINQTDGNILITDN
                     NGYILSGDKTDWSFSYKKYNDLISDEYLYKRPRLMSDVLIPSLESIYFEIIDNELILT
                     WNTSKEINCKGFVIERAEVVSDETDKNWKKIGYEKGDNNSDELNKYSFVDEKLKKGRF
                     QYRLKQESYDGSFEYFNLSEEVIWMDFPDKFQFYPVYPNPVTDSFKVRFFLPEKDEIR
                     LFFLNGNDTLYLLNNELQNQGFYKMEIDKNSLGFNNEVKRLYIVRNKFKKKVYYGDIR
                     FN"
BASE COUNT         4597 a         2024 c         2324 g         4443 t
ORIGIN      
        1 cgccactctt tcacagtgaa aacacgggtt cgaatcccgt tgggaccgct tgatttaaaa
       61 tcaaagctct gtgactgata aagttacagg gcttttgttt tagtaaagca ccgatctatt
      121 tttataaaat catctgttag gtttatataa tttctccggc aaacaaaaaa ttctttctgc
      181 tcactataaa accattgaaa ttaatacctc tatataattc agttaaaacc aagttaaatt
      241 gcaaaaactt aaatttttaa tatataaaat gtcttaaaca ggattaacat aatttttcca
      301 ttttttttat tttcaaatag gactatatta gtccaaatta gaatttaatg atcagaattt
      361 ccaaaaaagt tgaatatgca ttgatggcat taaagtttat atcagaatca gataacaggt
      421 tagttactgc aagagaaatt tccgattcga aaaaaatccc ttatgatctt ttatcaaaaa
      481 tacttcaaaa attaaaaaat gaagagatcc ttttgtcaaa tcaggggagt aacggaggtt
      541 attcaatgaa taaaagaacg gatgaaattt atctttataa ccttatgagt atgctcgatg
      601 gagaaatagc aattgcagat tgtcttgctg aaagtaaaga caaagactgc tgtcttgaag
      661 attcctgttc tattaaatct cctattacga aacttcagaa agaaattgaa aaccttttta
      721 aaaataaaac tatttctgat tttgtctgaa aaatttaaaa ttaactgccg atgaaatttc
      781 ctgtttacat ggattataat gctacaactc ctgtagatcc tgaagtactc gaagaaatga
      841 tcccttattt ttgtgaacat tttggaaatg catcaagtgc taatcataag ttcggatgga
      901 atgccgagga agccgtttca aaagcaagaa aaaatattgc tgatcttatc aacgcatctc
      961 cgcgtgaaat tatttttaca gcaggttcga ctgaagcaaa taatatttct cttaaaggtg
     1021 tagcggaaat ttatgaagga aaaggaaatc acattatcac ctgcacttca gagcataaag
     1081 ccattttaga aacatgcgag tatctggaga caagaggttt tgaagttaca tatctgaaag
     1141 ttgataaaga tggattgata gatcttgacg aattaaaaaa tgcgataact gataaaacga
     1201 ttttagtcag tatcatggct gcaaataatg agatcggaac tattcagcct gtagaagaaa
     1261 ttggaaaaat atgcagagaa aatggagttt tgtttcatac agatgctaca caagcagtcg
     1321 gtaaaattcc tgtggatgta cagaaaatga acattgatct gatgagtatt tcagcgcata
     1381 aattttacgg accaaaaggt gtcggggcat tatatgtaag aagtaaagaa ccacgtgtaa
     1441 attttaccaa gcaaatgcac ggaggaagtc aggaaaaagg acttagaggc ggaactttga
     1501 atgtacccgg aattgtcgga atgggaaaag catgtgagat aagtatgaaa aatatgaata
     1561 atgaaatgtc gaaacacatt gtattcagag atagaataat tgatgcactt ttaaatataa
     1621 atgatgttta cctgaacgga cacaggacaa atcgtctgac gaacaatatt aatttcagca
     1681 ttggtaaaat aaatatagaa agtcttctcg gagatctaaa ggatattgcc ttgtctaccg
     1741 gaagtgcctg tacatcttca tcaatggcac catcacatgt attaagtgca attggcaggg
     1801 aagatggtct tgcaaggtct tccataagaa tcagttttgg gagatttacg actgaagaag
     1861 aaatagatta ccttattgaa aagctgactt caaatatttt aaagctaaga gaagaagctc
     1921 cagagtttca gattgcaaaa taattttaga taaaaaaata attaaaatat ttaaataaat
     1981 atattatgac agaagtaaaa gaattcattc cgggagtcag tgaagatgtg aatattactg
     2041 acaaagctat aaaggaagta aaaaaaataa tggaagaaaa taaagtacct gagaattacg
     2101 gactaagagt aggtgtaaaa ggcggcggat gttcaggtat gacctattca cttggatttg
     2161 atccggatga gagaacaggt gatacagtcc tcgaaaaaga cggtataaaa atatttatag
     2221 atatgaaaag tgttttatat ttatcaggaa ctgaaataga ttttacagac gggcttaccg
     2281 gaagaggctt tgagtttaat aatcctcaag ctgtgaagac atgtggttgc ggtagttcat
     2341 ttggagtcta agttaaattg aaaaaacagc tctcatattt tttactgttc ataatttttt
     2401 gtaattattt gcattaaaat aatgacctgt attttttgtt ttcttgggga atgaatgcta
     2461 taaataagta gaaaattttt aatcggatgt attattaatt agggatcatt tatattccgc
     2521 agaaataaaa taaaactgaa acagaaattc aattaattaa tcataatcaa aaaggagaaa
     2581 ttactaaaat ggcttacgaa tggaaattta atccaagacc ttacacagac gatgaagcat
     2641 cagaattgct ggcagaagta atagatgctg atactgcgaa ctggcattac aatactcatc
     2701 ataaaggtta tgtaactttt atgaataaga tcgaaactga acttgaaact gcagacagag
     2761 cttcagcaaa cggaaattac agtaatgttg gtgaactaaa aagaagattt acctggaatc
     2821 atgcaggtgc attacttcat gatgtttact gggaagtttt aggcggtgac ggcgacacct
     2881 caaaagggcc tgaagtagtc gcagctatag aaaaagaatt cggatcgatc gataactgga
     2941 aggctgattt caaagcgaca gcagtttcag caaaactttc aggctgggca gtattgactt
     3001 atgatcagct ttacagtgaa agacttttag atattcttgt tgatgaacac cattatggtg
     3061 caatctgggg tggaattccg ataatctgct gtgatgtttt tgagcatgca tattatcata
     3121 aggacggtcc cggcagagca acatatattg ataacttttt caaaaacctg aactggtcaa
     3181 ggatcaatga taggtataaa aagtatgcag taaaataaga aatatttaat tattttgtga
     3241 agggcatttt ataaatgccc ttttatttat ttacacaata taaattttta tttttgaaaa
     3301 gattttttgc atttacaata tttattttca ttttatcttc agtttttata tcctgtgaca
     3361 aaacagatac tgagcaaaca gagatcaata ataaagtatt cagttggaga cctgatctaa
     3421 aaataactga catacctgat actcctgtaa aaggattttt aaaaggtaaa gaaatcgaat
     3481 ttacttatat taattttgaa gactggagag gaagtggtga caatgtttta aatttcagta
     3541 accttatccc gaaaaacagc tgcggattta ttgagaatga taattcattt caattaatga
     3601 aaaaagcagg tgagattaaa gtgggagaat tgataaaatc ttcattcgat cagaatcttg
     3661 atggatttac tgcatattat gaaattgcag attccacaaa ccctgtaaaa tcaactgttg
     3721 agtggaattg tatcttagtt ataactgcca aaaatgaaaa aactattaaa ggaaaaattg
     3781 caatgtgttt taaagatgat acaaaaagct ggatagccgg tacatttgaa gctataagat
     3841 gttacaatta aaaaattatt aataacgatt tttatacatg aaaaaaatta tattatctgt
     3901 tttttttatt tcagcgctta ttttaacagg ctgtgataat aaagaaaagg aagccgtaaa
     3961 agaaagtaat gaaactgaaa aagaaatgac tgaaaagagc gagaatcaga ttaaagaaga
     4021 attaaacaaa gatgaatctg aaaattctga agatcagtca aaaattgaaa atgaacttgg
     4081 aatgaagccg ggtcttcctg ccgattttcc aaaagaaatt cctcatccga aaaatgccaa
     4141 agtattaggt tctctgaatt cttccgatgg tactgtcgtt acttttgagt caaatgataa
     4201 agttctagat attgtagaat tttataaaga ggaaatgaaa aaaggcggat atactttaac
     4261 agaggaaggt gagtcacttg tcagtgataa aggcggtctg ataaactggt caaaaggcaa
     4321 taagattgcc ggacttatgc ttgggtttga taaagataaa aatataacat cgctcgttat
     4381 cacatataat tagtacaact aaaaatattt gaaaaaactt ttgagtgaga gtgattcatt
     4441 cgaaagtttt ttttatttta aattctgaat ggatgaattc acagatctgc cattatttcc
     4501 tctgaatgta gttatgtttc cgcatgcaaa aatggaactg catatttttg aagagagata
     4561 caaaaaaatg ataaatgaat gtattgatga aggtaaaatt tttggaataa atttgtttgc
     4621 aaatgaaaaa atttttctga cagggtgtac atctgaaatt agtgaagtga aaaggaaaac
     4681 tattactgga gagtttgata ttgtttcgaa aggaattaga agatatgaga tcatgaacta
     4741 taaaatgact gctaataaat attttgtagg agaggttata ttttcaaaga attcaaattt
     4801 tattacagat gatattaagt ttcatgaagc agtaaataat tacaatgagc ttaccgacat
     4861 tgtctataaa ggcagtataa agccgattga tccgaatatt gaaaaatggc agacgggaga
     4921 attctctcct tcatttctga tggctgaaaa atgcggtttg agtgttgcag aaaggcaaac
     4981 tctgcttgag ataaataatg aggatgacag aatatttttt atgctgaatt attttgaaag
     5041 cattatgcct aaacttaaag acgctgacag gattgctaag ataataatga gtgacggtta
     5101 tctggttcag taaattctga ttttatattt atgatcgaaa aagacaaagt taaaaatatt
     5161 ttagttacga gaactgacag actcggagac gtgatactta ctttaccttt gatatcttgt
     5221 cttaaaaaga tctttcccga atctaaaatt tttttccttg taaaagttta cttaaaggat
     5281 attctggatt gttatgaagg tattgatgaa atcatttata ttaataatga agaagataag
     5341 gatattctgt cagaaattat cagatctaaa aatattgacc tggcaataaa tgtgaaacct
     5401 gattataaac ttgcattaat gctgtaccgg ctggggataa gatacaggat aggtacggct
     5461 tacaggtggt tctcattttt gtataattgt aaagtccatg ttcacagaaa acgctcagat
     5521 ctccatgagt cagaacttaa cttactaatg ataaaaagtt tttttgacga agaatgtagt
     5581 tcagagtttg gaaaatttaa aatagaagaa tcaaaaaagt taagactgga tcaaaaactt
     5641 tcagagttta atctggaact ttcggatact tatataatca tacatccggg cagcggaggt
     5701 tctgcaaagg atctgcctgc agaaaatttt attgatttta taaatatgtt cagtagtgag
     5761 tatagaaata ttaaaatagt atttactggt ttgcaaagcg aaaatgaact ggctgaaaag
     5821 ttaatcaatg gatccggaat agaaaaatcg gttgtaaatc tatgtggaaa gcttgacctt
     5881 ggagaaatgc tgatactgat taatgaatgc agtctcttta tttctaactc taccggacct
     5941 attcatattg ctggtgctct aaataaaaag atcattggct tttatccaaa tgaaaaacca
     6001 atgcatgcag gcaggtggaa acctcttgga aaagatgtta taattatttc acctgaatct
     6061 tctatagatg atctgagtga aattgataca aatgaaataa ttaatgcagt taaaaaattg
     6121 atttaaattt tttaatttgt acagaacaat aattgagtaa cgttgttaaa gattttgttt
     6181 ataaaattaa atttaaataa ttataatgaa gataattaga tcagtaatct taataactct
     6241 tttattcaca tcagtatttt taaatgctca ggataatgaa ttcagaacaa tcgaacagga
     6301 tgcatttaca accggtgaga aattaacatt tgaaataaat tacggattcg taaatgcagg
     6361 tacggccgta atggaaattg atcctaatct tcagactata aacggaagag aatgttatga
     6421 tattagattt actgtgaatt catcgggcag ttttgactgg gtatataaag tccgtgattt
     6481 ttacaggact tatgtagata aagaaggatt atttccgtgg agatttgaac agcatataaa
     6541 ggaagggaat taccagagag attttgaagc cattttcgat caggaaaatc ataaagcaaa
     6601 aacctatacc ggtgaaaaag agccaaagaa atttgaaggt gaatttgata ttcctgaata
     6661 tacacaggat gcaatgagtg cattttattt tttcagaaca ctggatttta gtaaaatgaa
     6721 agaaggggac gagactctgc ttcagaactt ttacaaggat aatacttatc ctctgaaagt
     6781 aaaatattat ggtaaagaaa ccatagaagt accggcagga gaatttagat gtgtaaagct
     6841 agagccgtta gttgctgaag gcggattatt taaaagtgaa ggttcaataa ttgtctggct
     6901 tacagatgat gaaagaagga tgcctgtgaa agtaaagacc aaagttgtga tcggatctat
     6961 agatgcagat ctcagcgctt attcaggact tgcaggatct cttaattcta aaaaataatt
     7021 taaaaaattt cagataaatt tataaagcga attaaaaatt cgcttttctt ttatgaaaat
     7081 atcagagtta ttttattcaa tacagggcga aggcaaaaga gccggtcgcc cttctttttt
     7141 tatcagaaca aatcactgta atctcaggtg taaatttcca ggcggaaatc tatgtgatac
     7201 ttattatacc agttgggaac ctgacgactt acggaatctt ggggaattga acttagatga
     7261 cataattgct gaatataaaa aaattaagtg ctgtgatatt gtaataactg gcggagaacc
     7321 tacgatgaat gcaaatgatc tgtcagatct ttgtcaaaaa ttaaaagaat caaatcctga
     7381 ttcatttatt acaattgaaa ctaatggtac atactttgga gattttataa aatattctga
     7441 attgatcagc gttagtccaa agctaaaaag ttctgttcca ttaggtacgg aatatgaaaa
     7501 aatgcatgag aagaacagat taaattttga gacctttata aagtttaatc ttctcaaaga
     7561 aaactatctt aaagacatcc agtggaaatt tgttttcaca tctgaaaagg acattaaaga
     7621 gataagggaa atccagtctg ccgttgggtt taaagacagt gatatatatt taatgcctga
     7681 aggaatcaca gcagaagatt taaacagtaa aagattagaa acattagagg tctgcaaaaa
     7741 gaacaatttc aattattcag acagacttca catacttgcc tggggaaaca agagaggagt
     7801 ttaaattaat tgaaaattaa aaattaaaaa ttacgagtta cgatttacgg gttacgattt
     7861 acgggttaat tgtgataaca aaattcatca ttcaatattc caaattccta attcttaatt
     7921 cttaattgat taaaacccca cagaataatt tatcctacct gcatctttgt tgacatcaaa
     7981 aaatacatta gatttttgaa gtaaggtatt atttgaattt ctgaaaaaat gatttatcac
     8041 atctgctgct gctattgcaa taaacataga agccggagca actccaggac tatatgaatc
     8101 tgtcctttca ctctgattaa acagatcatt tttagctttg ataataggag ctctcaatga
     8161 ataaataaga gcagcactgc cagctccgat cagcattcct gtgaatacat ctgaaggata
     8221 atgaaccccc cagtaaattc ttccaagtga tacaactgct gcataggtgt ataatccagc
     8281 tatcaaaacc ggttcaaccg gatatctcaa agtgagtgaa gtagcaatcg caaaagcttc
     8341 tgatgaatgt ccggaaggaa aagaataagt accttttaca gataaagtat ctgataagta
     8401 tacattgttc attgttctga atggtctggg tctttttact gtgactttaa ttatttgagt
     8461 aaccgacaaa ttcgtgatct ccgataaagc cagcaatatg gcagagcttt cgtcgtaact
     8521 attattgttg attcttgcaa aagtgtacat tcctacaggt gcagcaattg atattggtat
     8581 tatagattcg tttgtaatat tcaccattga atttatgaaa gagctgtgaa tattattaaa
     8641 agtccggaat aattttacgt caatactttt ttcactgtat ttcgttttat ccttacctga
     8701 ggtcttatat ctatattgag aaaaactatt tacataaaaa aataagatca gaattaatat
     8761 gaaggaaatt cttcgcaata attataattt actcagagca ttattgatat cagctaacag
     8821 atcttcaata tcttctatac ctactgataa tcttacaagt ccgtctgtca atccgaattc
     8881 ttctctgaga tatttcggaa cagtaccgtg tgtcatagaa acaggatggc agacaagtga
     8941 ttcaactcct ccaagcgatt cagcgatctg aaaaatttcc agagcgttac agaagtcaac
     9001 tgccttttcg tatgatccga cgtcaagtga aatgatccca ccaaaccctg acatttgtga
     9061 ttttgccagt tcgtgctgtg gatgagattc caaacccgga taataaagtt tttcgatcat
     9121 tgaattttca gaaagtactt tgcaaatagt aactgcattc tcgttatgag ctttcattct
     9181 ctgtgctaat gtttttgttg aacgcagaat cagccagcag tcaaaaggag caggaacaga
     9241 gccaattgag ttttgcaaaa atttaaaagt ttcggcaata gtttcgtctg aagtaacaag
     9301 gcatccgccg attacatcac tgtgaccgtt aatatattta gttgtactat gtaaaactat
     9361 gtcgatccca aagtccagtg gattctgaaa atacggactc ataaaagtat tatccacgca
     9421 tgttgtgatt tttttctcgc gtccaatctc tcctaaattt ttgagatcag taatgttcag
     9481 cattggattg gtaggagttt cgacataaat aaattttgta tttggtttta tagcattgat
     9541 cagtgcttca ttgtcagaag tgtcaacata cgaaaactcc agtccgtaat ttttcatgac
     9601 ctgttcatac aaacggtaag taccgccata gacattatta gtacagatca catgatcacc
     9661 cggtttaaat aaatttataa cagtctgtac ggctgccatt cctgaagaaa agcaaaatcc
     9721 atacttcccg ttttcaagtg cagcaaggtt tgcttcaaga gttgatcggg tcggattaat
     9781 tgtccttccg taatcaaatc cttttgaatc accaagtttt ttattggaat atgtagaggt
     9841 ctgaaagatg ggagtaatta cagctcctgt agtttcttcc ggatgctgac cggcgtgaat
     9901 ctgtttggtt gcgaatttca taatttagtt ttttttagcg aaaagtattt tcaaaaattt
     9961 taaatgaaaa tcctgaccgt atatttaata aaatttttaa tcagttttaa attaaacaaa
    10021 atcaagaacg tcatatctgg taagtattcc ttttatttgt ccgtgctgag atacaagaat
    10081 agtactgtct ttctgaagca tttcaagtgc tttcggaaat tcggtattaa aatccactat
    10141 cggaagtttt ttatccatta catcttttac ttgtttacct gatacattcc tgtcattcat
    10201 aactttttcg atgacacttt cctcagcaat agaaccaaca caatcagatc cgtctatgac
    10261 aggcaatgta ctgagattat atttatccat cagctcaagt gcttcgaata atttatcaga
    10321 acttttcgct gaaataagtc cgggcacacc agcagttttc ttagtcagga atacctgccc
    10381 tgttgaaatg aaatcagaat tcatcagacg cttttctcta agccattcat cagaatgata
    10441 tttagaaaga tatctttcac ctgtatcaca aactataaaa actataacag cattttcatc
    10501 aagggtttta gcaagctcaa cggcagcata agctatcgtc cctgttgatc cgccgcagaa
    10561 tattccttct tcttttccaa gtcttttaca catttctaca gaatcaagat cgttaacatt
    10621 aactatttca tcaatgtact gaagctgaac attctccacc atcacatcct gaccgatacc
    10681 ttcaattaag taggggatag gttcaggtgt attgccggtt tcttttacaa ctttaaatat
    10741 agaaccgtaa ggatctgccc cgattacttt aatacccgga tttttttctt taagatattt
    10801 ccctgtaccg gaaatagtcc cgcctgtacc gacaccggat acaaagtgtg ttatctttcc
    10861 ttctgtatca ttccagatct cgggacctgt agttttgtaa tgcgcttcag gatttcccgg
    10921 atttacatat tgataaagaa aaagtgaatt tggaatttct tttgataatc tttcagctac
    10981 agttacatag tgatcgggtt ctccatgctt agcagtcatc ggctgaatta tcacttcggc
    11041 acctagtgct ttgagataat tatttttttc tctggaagct ttttctgtca ttacaaaaat
    11101 tgtcttataa ccttttaacg cagctaccag tgcaattccg atccctgtat ttccactcgt
    11161 agcttcgatt atagtgccgc ccggtttgag cttgccttca cgttctgcag cttctatcat
    11221 atttagtccg atcctgtctt ttacactacc gccgggattg tatgactcca tctttgcaag
    11281 tacagtcggt ttcagacctt ttgtaatgtt gttgagtttt acaaggggag tgttgccgat
    11341 cagatcaagt acgctgttat aatatttcat atttataatt ttgtaaattt aaatttgata
    11401 attctatttg ggtatgttga agtctgaata aaatttttat tcaaatttta tatctcctgt
    11461 atattctttt cgtctcatac tctttctagt cagataaatt ctttttactt cattgtaata
    11521 tttgaatgta tttttatcta tagatttttc ataaaatccc tgacccagtt ttattttttt
    11581 cattacatag acagtatcag ttccattaat aaaaaatata ctgatctcat cattcgaggg
    11641 gagaaaaaat ctgacattaa atttttcttt taccgggttt gggtataccg gataaaattg
    11701 taaagtagtt ggaaattcca tccagaatac ttctttattt aaattcaaat attttgaagt
    11761 cccgtcattc atatccatct taattctgaa tttgtatttc ccttctttta attttttatc
    11821 aatgtatgaa tacttgcttg tggtaggagc tgtctttaca cctctgacat aatcgatctc
    11881 tacccagtca gattcagaac tgtccggttt gatttctatt ctttctactg caaaaccttt
    11941 acaggtaaaa cctttgggag tgttccattt caacaataat ttatttccta ttatttcatc
    12001 tactattttc caatcaggtt gattcttgag attagtatca tttacaggat ttacaaataa
    12061 attctcaatt gcatttattt tattgtaaga cttgtcatta tatgtccagt cttttttatc
    12121 accgtctaat atatttccct gattgtctgt gattaaaata aattcatcag ttttaacact
    12181 ttccgttttt ttctgagaat aaagactttc gttgctgaaa aatataactg tggataaaat
    12241 gaatatgtaa ggtagtatgt gttttaaatt tagtgtggtc aattatttaa aatttattat
    12301 tgtaattttt tgaatattat tttaaaggag tacactttaa aaattgtttt gcatttcttt
    12361 ctgacaaccc caccctgccc tccccttggt aaggagtggg tttctgatgg tctttgatag
    12421 aatcaacttc atgtaaagtt gaatattaca agtcctcaat tgaatcttat atctccgtaa
    12481 taaactttct ttttgaattt atttcttaca atgtataatc tctttacttc gttattaaaa
    12541 cctaaagaat ttttgtcaat ttccatttta tagaatcctt gattctgaag ttcattattt
    12601 aataaataca aagtatcatt cccatttaaa aaaaataaac ggatctcatc tttctcaggt
    12661 aaaaaaaatc tgaccttaaa tgaatctgtt actggatttg gataaacagg ataaaactga
    12721 aacttgtccg ggaaatccat ccagattact tcttcactta aattgaaata ttcaaaactg
    12781 ccatcatatg attcctgctt aagtctgtac tgaaatctac ctttttttaa tttttcatca
    12841 acaaaactgt atttgtttaa ttcatcggaa ttgttatcac ctttttcata accaatcttt
    12901 ttccagtttt tatctgtttc atctgaaaca acttctgctc tttctatgac aaatcctttg
    12961 cagttgatct cttttgaagt gttccaagtc aatattaact cattgtcaat aatttcaaag
    13021 tatatagatt ctaaagaagg aattaataca tcagacataa gtcggggtct tttatataaa
    13081 tattcgtcac ttatcagatc attatacttt ttatatgaaa aactccagtc cgttttatct
    13141 ccacttaaaa tatatccatt gttatcagtt attaaaatat ttccatccgt ttgattgatt
    13201 tcattttgag aataaacatt tttattgctg aaaaatatta cagcggaaaa aatgattatg
    13261 tatatttcgt gtctcaaaat ttgtcggttc atttttttaa tttcttaatg tatctttttg
    13321 aatatttttt ttaatttata ttaatttttg taattgcatt tatagtttgg agtttagact
    13381 catcttat
//
LOCUS       DAORVQ010000089        13354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2178_length_13354_cov_5.217836, whole genome shotgun sequence.
ACCESSION   DAORVQ010000089 DAORVQ010000000
VERSION     DAORVQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_13354_cov_5.217836"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PLX80_08480"
     CDS             <1..1069
                     /locus_tag="PLX80_08480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQY20862.1"
                     /translation="EKSTEVKKIICKMGEGIAGWVAQRREPLLIDDCYKDERFNPESD
                     KRNNFITRSMICVPMIHKEKLVGVIQVINKKNEDTFSERDLMVFKILASQCAVAIENA
                     KLLEIQIEQEALNRELKTAREIQQNLLPVKLPEFKDLDIAAVLIPAKEIGGDYYNVFK
                     ISDDKTLFIIADVTGKSISAGLIVSTIYAAVITYFDQNQEKFELKDFVNCLNRILLKS
                     TTAEKFATGWFGLYDHSSKTLESVNAGHNVTYVLNSNAELSELKTGGLFLGCFDLEYI
                     SESVTLNSGDVVFYFTDGVNEAMDSEQNQFGDDRLEKLIRENLNNSADSILQYLLKDI
                     DKFVGDAEQSDDITCGLIKVL"
     gene            complement(1125..1619)
                     /locus_tag="PLX80_08485"
     CDS             complement(1125..1619)
                     /locus_tag="PLX80_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20863.1"
                     /translation="MNHLKKLSRLQIGIFSLLIAICGVLGYLYYDSYKTNIKDSLALK
                     EVKAENLDLSQDVDIVKDKYDRLQKEVDVLKGKVKKVTYRKKYTKRKSKLSSAGKRYS
                     GKKMSYKQLYYNLKNKCSVNKHSKKKYSSKKYSKKKSSYKRSNTSTKKYNTKYKPYTN
                     FRNR"
     gene            complement(1814..3811)
                     /locus_tag="PLX80_08490"
     CDS             complement(1814..3811)
                     /locus_tag="PLX80_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY20864.1"
                     /translation="MKNLTNLILIILVNFLFSIHANSTPINQFINTVSPALNANSIVK
                     SSDIVISFEQIMNGSTMTSANVKVFGYQTGLLTTSLDFNSVANTLTINPVNEFKNGEI
                     ISVTLTAGVKTISNQSITPFVYKFRAKALGGTGSFILASEINNTTYGNIRSGDLDGDG
                     DLDLVINNGIYINNGNALFNLSSTLNNQGWPEIFDADNDNDLDILLTYDSTIYYYKND
                     GLGNFSLFSTFAGSVWGYGDLTGNGFLDFAYFVNASEVKTAFNNNGIFNQAQSYSLSG
                     LCFTSANYTDNLLFDDFNNDGSIDIMSISGYAGGNSITGYDLCKSFNQLTNIGTGNFT
                     VQTIFDSFNGGGPYIFSAGDSKSFDFDGDGFIDITSPFLKLINNDNTTYSDAGYLFPF
                     SNSLDFDANGDEFIDICVNYYGVFSCYINNGIGDFTPSFIIQKPSWGSKAASGDFDND
                     GDIDMAIIYYDDTKVLILLNGDSPLPVELSSYTSSINQNSVKLNWSTSQEQNNSGFEI
                     QRADKFTEGQKVWKKIDFINGAGNSNDEKNYSYDDKNLTSGKYKYRLKQIDFNGNFEY
                     HELSNEVVIGIPSSTELMQNYPNPFNPVTNISYRLSENGFVTLRIFDNTGREVKTLVN
                     EFRQAGYYNEIFNGSDLASGVYFYKLTTGDFVQTKKLSLLK"
     gene            4015..5469
                     /locus_tag="PLX80_08495"
     CDS             4015..5469
                     /locus_tag="PLX80_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQY20865.1"
                     /translation="MKKTFIILIYIILMNLSFVRAQFNPDSLEIKVSRIVKEYVDTNK
                     AGMVIGVIRAEGNDVMSKRFSFGHVRKDTLSPRPDSLTIFHIGSVTKSFTATILSSLI
                     QQGGPLNLNDLVEDHIPLNTVKAPVFVTTGGDTIKMKIIDLATHYSALPDDPIHPVND
                     STTYQMMYNYLNNHRLSRTPGECFLYSNLGVSFLGVVLTHTLGKEIDSLFIQKISDPL
                     GLPDTRIFLSQQQDLRRATGYGPNGDSAGYFKDSWPAFYAAGGLYTTIKDFTKYLEFN
                     MGLINNGMQNILDSAHFIRRVSNDTCHNPNSKGRVGLVWQMSPLFPELSNNFYYTWKD
                     GGVPGFSSFICFTTDTAKNIKSGVVMIVNQSIPVDKLATDILKYLNSDISMSNVVTDQ
                     EIPSGFNLDQNFPNPFNPKTIINYELRVSSDELVSLKVFDVLGNEVAELVNEKQNVGS
                     YSVEFDGGNLSSGIYFYTLQAGDFSQTKKMTLIK"
     gene            5697..6392
                     /locus_tag="PLX80_08500"
     CDS             5697..6392
                     /locus_tag="PLX80_08500"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY20866.1"
                     /translation="MSLKVIGSGLGRTGTYSLKLALEHLGFGKCYHMLELFQKPNGVK
                     HFWDAENGKSVNWEELFTGYDSAVDYPVARYYKQLAEFYPEAKVIHTIRNPEEWYESA
                     KHTIFNAKNLDGIKLLKFAAKFPFSKEVRKRFKVFKYNRNLMDQEFGKDLTNKDKVIK
                     AFIKHTENVKKEIPPERLLLYRSSEGWESLCRFLNVPVPDEEYPNRNSREEFLNRVDS
                     IGTGKFIEPQKVI"
     gene            6470..7606
                     /locus_tag="PLX80_08505"
     CDS             6470..7606
                     /locus_tag="PLX80_08505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY20867.1"
                     /translation="MKNFFKNYRSVVTCIAVYFLLLSSGLSEKVLAQINSESITRSLN
                     SYIDSVYNAENLPGVVAEIFTPDFTYQYKVGKGDLQTGVERRYDDKIRVGSITKTFTA
                     TVILQLIQEGKFALEDPLSKFFPEYPNSENIKIREILDMTSGIPDFLEDPTLGNDFFY
                     NRTAKVTEREIFDATIELGPAFPPGTSWQYSNGNYNILGMIIEKVTGNKVGDEITNRI
                     IKALGLVNTEYPVENFITGDYSHGYMTDTATGKIIDVTIIDPSITGAAGCIVSNFTDL
                     KIYGLALAKGSMLNKEMQEQRLKFVKTGIVDFATYGLGIFDLGGFIGHNGGITGFNTM
                     MCYNPELDALFLVSVNEFGPKGGAVDKIFMELAKIVYPDKNLFK"
     gene            complement(7782..8489)
                     /locus_tag="PLX80_08510"
     CDS             complement(7782..8489)
                     /locus_tag="PLX80_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQY20868.1"
                     /translation="MSKQNLNFKDNEVHVYQINISKHIDQISDLRKILSKDETEKADR
                     FKIEKDSKQYIVSRGSLRKILSEYIHIDSYSINFSYTEFAKPFLENSKIHFNLSHSGD
                     WCCIAVSLIKDIGIDIEKVREMEDVSKIAERYFALNEFEHIKNFEGTDQINEFFRIWT
                     LKEAFIKAVGEGLSFPLKNFSVISDKNESPVLNLFENKDEVKDWKLCDLECEEEDYYS
                     SIALKSEKKIDLIYKYL"
     gene            complement(8505..12575)
                     /locus_tag="PLX80_08515"
     CDS             complement(8505..12575)
                     /locus_tag="PLX80_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HQY20869.1"
                     /translation="MDQDLKNKIEDIYELSPMQQGMLFHTLYEEGSDAYIEQFCYDLE
                     GELDASAFEKAWNEVVKRHGVLRTSFQYKGISKPVQIVNKSVSLPWENLNWSNLKSED
                     IETEFKTFIKADRSKSFSMESAPLMRCTLIKLNDQLYKFIWSFHHILMDGWSYPVIQK
                     EVFAFYESFKNGKAAELSNPISFKQFIVWLNTTDKESAKNFWTNELKGFGSPTPMIFN
                     KNKNSHTAEKNADEIKDIEVKFSEELTSNLNSFVKENKLTLNSVIQGIWAFILSKYRN
                     EKDVMFGGTVSGRNPLLKGIESMVGLFINTLPVRVDISGESKVINWLKELQLKQIERD
                     QYSYSALVDIQEWSEIPRGTKLFENILVFENYPVDNSLKEGIAGIRIKDLKAIERTNF
                     PLTVLVAPGNELTIHIAFETSKFSDEFIKNILSSFESLTKQIIAKPDSKLSELVILSE
                     DEKNKILYKWNDTSKELDNKCVHKLFEDSVEKYSDNIALESGKNKLTYKELNQRSNQL
                     AHFLIRSGIKPGDTIGISLKRSEEMIITLLGILKSGAAYVPIDPTFPKDRINYLLKDS
                     GIKILITSSDLSENFENGDHRTIYFDNEISKLENESTNNPQTSVSPDDLVYVIYTSGS
                     TGKPKGVLMMHKALSNLLNWQMDSHEFKEGFRVLQFTTLSFDVSFQEIFSTWISGGTL
                     VLLTEEERKDLSEVASILKTKKIQRIFQPFVALQGISEVCSVSNINDLELKEVITAGE
                     QIQITPALLEFFNNLQGSTFSNHYGPSEAHVVTSYTFGNDTSAWPDHPPIGKPISNNR
                     MYILDDTLEPVPVGVTGDLYIGGISLVRGYLNRPKLTKEKFLDDPFIKGETIYNTGDL
                     ARYLENGDIEFLGRADSQIKLRGYRIELGEIENVISEYEGIKNTAVIAKEFSNGEKKL
                     FAYIVISDKNINTDEIKDKLISFLKTKIPEYMIPHDFIFIDTMPLTGTGKVNLKALPE
                     PELSKTGGGDNYVVPNDPLELQLVNIWEKVLGIKNIGIRDNFFELGGHSLLAMRVFGY
                     IEKLTGKKLALSTLFSSPTIEQLANILKDDGWKPNWKSLVAVKPGGSKLPFYCVPPAA
                     GTALHFQDMVKYIPDDQPFYILESVGLDGKEEPHNDIKEMATFYIKEIQSLQPEGPYL
                     LGGRCFGGRVVFEMAQQLIKQKQKVALLAIFDTWPPFTETPKDYVQPTRDTSHIVSRS
                     MHHLKSGEFFKVAWNFTKYKASKINWKIKNKIEYLFSDELSRKYKEIMLMHFKAQDRY
                     IAHKFPGKITLIECATFRQDFRDQWKELAGGGFETYVVPDTDHKTIVKEPKLKDFAEK
                     LNIVLQKTHDEINGLQSTAKVNSSAVTEDKTELSKV"
     gene            complement(12579..>13354)
                     /locus_tag="PLX80_08520"
     CDS             complement(12579..>13354)
                     /locus_tag="PLX80_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HQY20870.1"
                     /translation="NIVTSEENVNIEFSKEDTGLILKDVHKSYNTQINDILLTALILA
                     FNKWNGENKLLLNLEGHGREDIADNSFSTDISRTVGWFTSVFPVLLKINSSKDTGECI
                     KSVKENLRNIPNNGIGFGLLKYMSDDTEIKKSLSDFPEVEVVFNYLGQMNFQFLPTDW
                     KIVEGLKLSQDGENKRFHSIEINAVVTDDKLLLEFNFSNNIYQRKNIEKLADHFKNSM
                     NGIISHCTGEDKNSGFTPSDFSAAGLDQQELDDLLANLN"
BASE COUNT         4285 a         2244 c         2248 g         4577 t
ORIGIN      
        1 agagaaaagc actgaagtaa aaaagatcat ttgtaaaatg ggagagggga tagccggatg
       61 ggttgcacag agacgagaac cgcttttgat cgacgactgc tataaagatg aaagatttaa
      121 tccggagtct gataaaagaa ataatttcat taccagatcg atgatctgcg ttcctatgat
      181 tcataaggaa aaacttgtag gtgtaataca ggtaattaat aaaaagaatg aagatacttt
      241 ttctgaaaga gatctgatgg tttttaaaat tcttgcatca caatgtgcag tggctataga
      301 aaatgcaaaa cttctggaaa ttcagataga gcaggaagct ctgaatcgtg aactgaaaac
      361 tgcgagagaa atccagcaaa atctgcttcc tgttaaacta cctgagttta aggatcttga
      421 catagctgcg gtactaatac ctgcaaaaga gattggcgga gattattata atgtctttaa
      481 gatatcagat gataaaactt tgtttattat tgctgatgtt actgggaaga gtatttcggc
      541 gggcctgatc gtttcaacga tctatgctgc ggtgatcact tattttgatc agaatcagga
      601 gaagtttgag ttgaaagact ttgtgaattg tctaaacaga atattactta agtctactac
      661 tgcagaaaaa tttgctactg gctggtttgg gctgtacgat cattcatcga aaactctcga
      721 aagtgttaat gccggacata acgttactta tgtgttaaat tcaaatgcag aattatctga
      781 attgaaaacc ggaggtttat tcctgggatg ttttgatctg gaatatattt cggaatcagt
      841 tactcttaat tcaggagatg ttgtgtttta ttttaccgac ggtgtaaatg aagcgatgga
      901 ttctgaacag aatcagttcg gtgatgatag actagaaaaa cttataagag aaaatctcaa
      961 taatagtgca gattctattt tgcaatatct tttaaaagac attgataaat ttgtgggtga
     1021 tgcagaacaa agtgatgata ttacttgtgg attgattaaa gttctataaa aaaaatgagc
     1081 ataaagaaac ttcctttatg ctcaatgaat gtttagaatt aaatttatct gttcctgaag
     1141 tttgtatatg gtttatattt agtgttatat ttttttgtac ttgtatttga ccttttataa
     1201 gatgactttt tcttactata ctttttagag ctatactttt tcttgctgtg tttatttact
     1261 gaacatttat tcttcaggtt ataataaagc tgcttataac tcattttttt accgctgtat
     1321 cttttacccg cagatgataa tttactttta cgttttgtat atttcttcct gtaagttact
     1381 ttctttactt tgccttttaa tacatccact tctttctgaa gtctgtcata cttgtctttt
     1441 acaatatcca catcctgtga cagatcgaga ttttcagctt ttacttcttt cagtgccaga
     1501 ctatctttaa tatttgtttt ataagagtca taatacaaat atccaagaac tccgcaaatt
     1561 gcgataagta aagaaaaaat tcctatctgt agtctggata atttttttaa atggttcatt
     1621 ttcttaaatt tgatttagaa taaattttat ccgcgtacgg gtaaactaaa atttggttta
     1681 gcaaaagtaa tattttgaaa agttaataac aaacaataaa aataattatt ttctaaatct
     1741 taatctaaca ggttactttt actttggact ccgcagagtc ttgcgctctt tggactctgc
     1801 cggtaagaac tatttacttt agcagcgata atttcttcgt ctgaacaaaa tcccctgtag
     1861 tcagcttata aaaataaacc ccgcttgcga gatcgctgcc gttgaatatc tcattgtagt
     1921 atcctgcctg tctgaattca ttcacaagag tctttacttc tctgcctgta ttatcgaaaa
     1981 ttctcaaagt cacaaaacca ttttccgaaa gtctgtatga aatatttgta accggattga
     2041 aaggattagg atagttttgc attagttcgg tggaggaagg aattcctatt actacttcat
     2101 tcgaaagttc atgatattca aaattgccat tgaagtcgat ctgcttaagt ctgtatttat
     2161 attttcctga tgtaagattt ttatcatcat aagaataatt tttttcatca ttagaattac
     2221 ctgcaccgtt tatgaaatca attttcttcc atactttctg accttcagta aatttatcag
     2281 ctctctgaat ttcaaagccg gaattatttt gctcctgtga agtactccag tttagtttta
     2341 cagaattttg atttatgctt gaagtgtagg atgaaagttc gactggtaaa ggtgaatcgc
     2401 cgtttaggag gattaaaacc ttagtgtcat cataataaat aattgccata tctatatctc
     2461 catcattgtc aaaatctccg gacgctgctt tagatcccca ggaaggtttt tgaataataa
     2521 aagaaggagt aaaatcacct attccattat ttatgtagca agaaaaaaca ccataataat
     2581 taacacatat atctataaat tcatctccat ttgcatcaaa atctaaagaa ttactaaatg
     2641 gaaacaaata tcctgcatca gaatatgttg tattgtcatt atttattaat tttaaaaaag
     2701 gtgaagtaat atctataaaa ccatctccat caaagtcaaa ggattttgaa tctccagcac
     2761 taaaaatata tggcccacct ccattaaatg aatcaaaaat agtttgaact gtaaaatttc
     2821 ctgttccaat attagtaagt tgattaaagc ttttgcataa atcgtatcct gtaatactat
     2881 ttcctccagc atatccactg attgacatta tgtcaatact accatcatta ttaaaatcat
     2941 caaataaaag gttatctgta taatttgcgg atgtaaaaca tagaccagaa agtgaataag
     3001 attgtgcctg attaaaaatt ccattattat taaatgcagt ctttacttca cttgcattta
     3061 caaaatatgc aaaatccaaa aatccatttc ctgtcagatc accataaccc catacacttc
     3121 ccgcaaaggt tgaaaataaa ctaaagtttc ccaaaccgtc atttttatag taataaattg
     3181 tactatcata agttaatagt atatctaagt cattgtcatt atcagcgtca aaaatttccg
     3241 gccatccctg attatttaga gttgaagata gattaaacaa tgcattacca ttatttatat
     3301 aaattccatt attgatcact aaatccaaat ctccgtcacc atctagatct ccggatctga
     3361 tattaccata tgttgtatta tttatttctg aagctaaaat aaaacttcct gtacctccaa
     3421 gagcttttgc tcggaattta taaacaaatg gagtgatact ttgatttgaa attgttttaa
     3481 ctcccgctgt aagtgtcaca cttattatct caccattttt aaattcattt acaggattaa
     3541 tagtcagagt atttgcaact gaattgaaat ctaaagaagt tgtcaacagt ccagtctgat
     3601 atccaaagac ttttacatta gccgaggtca ttgttgagcc attcattatc tgttcgaatg
     3661 aaataacaat gtcagatgat ttgactatgc tgttggcatt caatgcaggg gatactgtgt
     3721 ttatgaactg gtttatgggt gttgaatttg cgtgtattga aaataaaaag ttgactaata
     3781 ttattaatat taaattggtc agatttttca aaattactcc tttttaaaat tctttaaaag
     3841 ataacccata gattttttaa aatcaagacc cggaaagttg cttaagataa ttataaattt
     3901 caaaattcga gttacaagta attttataaa gtgtacattg aaaattcata attgtattta
     3961 aatattaaaa cggataattt attgaaatat tttataaccg ctaattaatt ttttatgaaa
     4021 aaaacattca tcattttaat ttatataatt ttaatgaatt tgtcttttgt ccgggcacaa
     4081 ttcaatcccg actcgctcga aattaaggtc agccgtattg tcaaagaata tgtagataca
     4141 aataaagcgg gaatggtaat aggcgtgatc agagctgaag gaaatgatgt gatgtcaaaa
     4201 agattttctt tcggtcatgt cagaaaagat actctttcac caagaccgga cagtctcaca
     4261 atttttcata taggatctgt tacaaaatct tttactgcta ctattttatc ttcgctgatt
     4321 cagcagggtg gtccattgaa tttaaatgat cttgtcgaag atcacattcc gttgaatact
     4381 gtcaaagctc ctgtctttgt aacaacaggc ggtgacacga tcaaaatgaa aattatagat
     4441 ctagctactc attattccgc attgccggat gatccgatac atccagtaaa tgacagcacg
     4501 acttatcaga tgatgtataa ttatctcaat aatcacagac tttcacggac accgggtgaa
     4561 tgttttctct attcaaattt aggagtttca tttctgggag ttgtcttaac tcataccctc
     4621 ggcaaagaaa ttgattctct ttttattcaa aaaatttcag atccccttgg attacctgat
     4681 acaagaattt ttttatcaca gcagcaggat cttagaagag caacaggata tggtcctaac
     4741 ggagattcag ccggatattt caaagacagc tggccggcgt tttatgcggc aggaggatta
     4801 tatactacga taaaagattt tacaaagtat ctggaattta atatgggatt aataaataac
     4861 ggtatgcaaa acattctcga ctctgcacat tttatcagaa gagtcagcaa tgacacgtgt
     4921 cataatccga attccaaagg cagagtagga ctggtctggc agatgtctcc tttatttcca
     4981 gagcttagta ataattttta ttacacatgg aaggacggtg gagtgcccgg attttcaagc
     5041 tttatctgct ttacgactga cactgcgaaa aatattaaaa gtggtgtagt gatgatcgtc
     5101 aatcaatcca ttcccgttga taagttagca actgatattt taaagtattt gaattcagat
     5161 atttcgatga gtaatgttgt gactgatcag gaaattccat ctggctttaa tcttgatcag
     5221 aatttcccta atccatttaa cccaaaaact attataaatt acgaattacg ggtttcaagt
     5281 gacgagttag tgagtctgaa agtttttgat gtattaggaa atgaagtagc agagttagtc
     5341 aatgagaaac agaatgtcgg aagttattca gtggaatttg atggtggaaa tctttcgagc
     5401 gggatttatt tttatactct gcaagccggg gatttctctc aaactaaaaa aatgactctc
     5461 attaagtaaa ttgcaaatta caaatttcgg gttaacagtt acgaattaag agttaatatc
     5521 tatatgtatt aagtatatga aactaagttt ggcaaccagc aaagatatac aatttatatt
     5581 ttattcttta tgtccttgtg tctttttgtc tctgtggtaa gtaaagattt tctgcactaa
     5641 taaagacagg gattggtgaa tgtgcggaat aggattattg aaatttatta aaatacatgt
     5701 cattaaaagt aatcggcagc gggcttggaa ggacaggcac ttattctctg aagcttgcac
     5761 ttgagcatct tggatttggg aaatgctatc atatgctgga gctttttcaa aagcccaatg
     5821 gagttaaaca tttctgggat gctgaaaatg gaaaatcagt gaactgggaa gaactcttta
     5881 caggatatga ttcagctgtt gattatcctg ttgcaagata ttataaacag cttgctgaat
     5941 tttatcctga agctaaggtc attcatacga tcagaaatcc tgaagaatgg tatgaaagtg
     6001 caaagcatac gatctttaat gcaaaaaatc ttgacggtat aaaacttctt aaatttgcag
     6061 ctaagtttcc attttcaaaa gaagttagaa aaagattcaa agtttttaaa tacaacagaa
     6121 atcttatgga tcaggaattt ggcaaagatc ttactaataa ggataaggtt ataaaagcat
     6181 ttataaagca tactgaaaat gtaaagaagg aaattccacc tgaaaggtta ctactttata
     6241 gatcttcaga aggttgggaa tccttatgca gatttttaaa tgtacctgta cctgatgaag
     6301 aatatccgaa cagaaattcc cgtgaagagt ttttaaacag agtcgatagt atcggtacag
     6361 gaaaatttat cgaaccacaa aaagtaatat aaattttaaa tgattggtta aaaatatttt
     6421 ttaataaaca ctataaaccc attaacttta ataaacttaa ctaaatagca tgaaaaactt
     6481 ttttaaaaat taccgctctg ttgttacatg tattgcagtt tactttttat tactttcttc
     6541 aggattatct gaaaaagtat tggctcaaat taattctgaa tcaattaccc gcagcttaaa
     6601 cagttatatt gattcggttt acaatgctga aaatttacct ggtgttgtcg ccgagatatt
     6661 tacacctgat ttcacatatc aatacaaagt tggaaaagga gatctgcaga caggagttga
     6721 aaggcgatat gatgataaga tcagagttgg cagtatcaca aaaactttta cagcaactgt
     6781 gatacttcag ttaatacagg aagggaagtt tgctcttgaa gatccgctta gtaaattttt
     6841 ccctgaatat ccaaattcag aaaatatcaa gataagggag atcttagata tgacaagcgg
     6901 catacctgac tttcttgaag atccgacctt agggaatgat tttttttata acagaacagc
     6961 aaaagttaca gaaagagaga tctttgatgc tacaattgaa cttggacctg cattcccacc
     7021 cggtacttca tggcagtatt caaatggaaa ttataatata ctcgggatga taattgaaaa
     7081 agtaacaggc aataaagttg gagatgagat cacaaataga atcataaagg cacttggtct
     7141 tgtgaataca gaatatcctg ttgagaattt tattaccgga gattacagcc acggatatat
     7201 gactgatacg gctacgggaa agataatcga tgtaacaata attgatccat cgataaccgg
     7261 agctgcagga tgtatagttt cgaattttac agatctgaaa atttacgggc tggcacttgc
     7321 aaaagggtca atgctgaata aggaaatgca ggagcagaga ctaaaatttg tcaaaacagg
     7381 cattgtggat tttgctacat acggacttgg aatttttgat ttaggaggat tcatcgggca
     7441 caacggcggt attaccggat ttaatacaat gatgtgttat aatcctgaat tagatgcatt
     7501 gtttcttgta tcggtaaatg agttcggtcc taaaggcgga gcagtcgata aaatatttat
     7561 ggagttagca aagattgtgt atccggataa gaatttattt aaataagatt tttatctgta
     7621 tcccagtctg agagctttgc agcagtatta gtatgaaacg gagtttcaaa agtattggcg
     7681 ttaccaaacg gagtttggta atgaacaggt gtggtgtgaa acagagtttg gcaattagct
     7741 ggtgtagaat aattcagaat ttggtaatga gctgtaattc attaaagata tttatatatc
     7801 aaatctattt ttttttctga tttcaaagct attgaagaat aataatcttc ttcttcacat
     7861 tccagatcac aaagtttcca atctttaact tcatctttat tctcaaacag attcaaaaca
     7921 ggagattcat ttttatcaga aattactgaa aaatttttta atggaaaaga aagtccttct
     7981 cctactgctt ttataaaagc ttccttcaaa gtccatatcc tgaaaaattc atttatctga
     8041 tctgtgcctt caaaattttt tatatgctca aactcattta gtgcaaaata tctttcagca
     8101 atttttgaaa catcttccat ttctctgact ttctcaatgt ctatgccaat gtccttgatc
     8161 aaagaaacag caatacagca ccagtctccg gaatgagata aattaaaatg aattttagaa
     8221 ttttctaaaa aaggttttgc aaattctgta taagaaaaat ttattgaata tgaatctata
     8281 tgaatatatt cagaaagaat tttcctgaga gaacctctgc ttacaatata ttgctttgag
     8341 tctttctcaa ttttaaatct gtctgccttt tctgtttcat cttttgaaag gatttttctt
     8401 aggtcggaaa tttgatctat gtgtttagaa atatttattt gatagacatg aacttcattg
     8461 tctttaaaat ttaaattctg ttttgacaat tttgacaatt aaagttaaac tttggaaagt
     8521 tcagtcttat cttctgtgac tgctgaggaa ttaacttttg ctgtagactg taatccattt
     8581 atttcatcat gagttttttg caatacgata ttaagttttt cagcaaaatc tttcagcttg
     8641 ggttctttca caatagtctt gtggtcagta tccggaacta catacgtctc aaatcctccg
     8701 cctgcaagtt ctttccattg atccctgaaa tcctgtctga atgtcgcaca ttcaatcaaa
     8761 gttatcttac ccggaaattt atgtgcaatg tatctgtcct gagccttgaa atgcatcagc
     8821 ataatttctt tatactttct tgatagttca tcactgaata gatattcaat tttatttttt
     8881 atcttccagt ttatttttga agctttatat tttgtgaaat tccatgcgac cttaaaaaac
     8941 tcaccactct ttaaatgatg cattgatctg gaaacaatat gacttgtatc tcttgtcggc
     9001 tgaacataat ctttaggagt ctcagtaaac ggaggccagg tatcaaaaat agcaagcagt
     9061 gctactttct gtttttgttt gatcaactgc tgagccattt cgaaaacgac tcttccccca
     9121 aaacatcttc ctcctaaaag gtaaggacct tcgggttgca gtgactgaat ttcttttata
     9181 taaaaagttg ccatttcctt tatgtcatta tgaggttcct cttttccgtc aagaccaaca
     9241 gactcaagaa tatagaacgg ctgatcatcc ggaatgtatt ttaccatatc ctgaaaatgc
     9301 aaagctgtcc cagcagcagg cggcacacag taaaaaggaa gtttggatcc gcctggcttt
     9361 accgctacca atgacttcca gttcggtttc cagccgtcat cctttaatat gtttgcaagc
     9421 tgctcaatag taggagaact gaataaagtc gatagtgcaa gttttttacc ggtaagtttt
     9481 tcaatatatc caaaaactct catagccaaa agcgagtgtc cgccaagctc aaagaagtta
     9541 tccctgatac cgatgttttt aatccctaaa actttttccc agatatttac aagttgcagt
     9601 tcaagcgggt cattcggaac aacataatta tcgcctccgc ctgttttaga aagttcaggt
     9661 tcaggtaaag ctttcagatt gacttttcca gttcctgtaa gaggcatagt gtctataaaa
     9721 ataaaatcat gagggatcat gtattcagga atctttgtct ttaaaaatga aatgagttta
     9781 tcttttattt catcagtgtt aatattttta tcagaaataa caatgtatgc aaacaatttc
     9841 ttttcaccgt ttgaaaattc tttagcaatg acagcagtat tttttattcc ttcatattca
     9901 ctaatgacat tttcaatctc accaagctcg atcctgtatc cacgcagctt gatctggctg
     9961 tcagctcttc caagaaattc tatgtctccg ttttctaaat atcttgcgag gtcaccggta
    10021 ttgtaaattg tttcaccttt aataaaagga tcgtctaaaa acttttcttt tgtaagttta
    10081 ggtctgttta gataacctct gacaagtgag atgcctccga tgtaaagatc gcctgtaacc
    10141 ccaaccggta caggctcaag agtatcatca agaatataca tcctgttatt ggaaataggt
    10201 tttccgatcg gaggatgatc cggccatgct gaagtatcgt ttccgaatgt ataacttgta
    10261 acgacatgtg cttcagaagg tccgtagtga ttggaaaaag ttgagccttg tagattgtta
    10321 aaaaattcca gtaacgcagg tgtgatctga atttgttctc cggcagttat aacttctttc
    10381 aattctaggt cattaatatt tgaaaccgaa caaacttctg aaattccctg aagagctaca
    10441 aacggctgaa aaattctttg tatctttttt gtttttaaaa tggaagcaac ttccgaaaga
    10501 tcttttctct cttcttcagt cagcaatact aatgtgccac ctgatatcca tgtagaaaaa
    10561 atttcctgaa aagaaacgtc aaaacttaga gtcgtgaact gaagtactct aaagccttct
    10621 ttgaattcat gtgaatccat ttgccagttc agcagattac tcaaagcttt atgcatcatc
    10681 agaacacctt tcggctttcc tgtagatccc gatgtgtaaa ttacataaac aagatcatcc
    10741 ggtgacacag aagtttgagg attattagta ctctcatttt ccagtttgga aatttcatta
    10801 tcaaaataaa ttgtcctgtg atctccattc tcaaaatttt cagaaaggtc acttgaagta
    10861 atcaggatct taattcctga gtcttttaat aaataattta ttctgtcttt cggaaatgtc
    10921 ggatcgatcg gaacgtaagc tgcacctgac tttaatattc caagcagtgt aatgatcatt
    10981 tcttctgacc gctttaaact gataccaata gtatcgccag gtttgatacc tgatcttatc
    11041 agaaaatgag caagctgatt tgatctttga tttaactctt tatatgttaa tttattttta
    11101 ccggattcaa gagctatatt atctgaatat ttttcaactg aatcttcaaa taatttgtga
    11161 acacatttat tgtcaagctc ttttgaagtg tcattccatt tatataaaat tttatttttt
    11221 tcatcttctg acaaaataac aagttcagaa agctttgaat caggtttagc aattatctgt
    11281 tttgttaaag attcgaaaga tgataatata ttttttataa attcatctga aaatttagaa
    11341 gtctcgaaag caatatgtat ggtaagttca ttacccgggg cgacaagaac agtgagagga
    11401 aaatttgttc tttctattgc tttcagatct ttgatcctta taccggcaat accttcttta
    11461 agagaattgt caaccgggta attctcaaac acaagaatgt tttcgaaaag ctttgtccct
    11521 ctcggtattt cactccactc ctgaatatcg acaagcgcgc tgtaagaata ctgatctctt
    11581 tcgatttgtt ttaactgcag ttcctttagc cagtttatta cttttgattc gccggaaatg
    11641 tctaccctta caggtaaagt gtttataaat aatccaacca tcgattcaat tcctttgagc
    11701 agaggattcc ttccggatac tgtgccgcca aacattacat ctttttcatt tcgatattta
    11761 cttaatataa aagcccagat gccctgtata acactgttta atgtaagttt attttctttt
    11821 acaaaactgt taaggtttga agtaagctct tcactgaatt ttacttcaat gtcttttatt
    11881 tcatcagcat ttttttctgc ggtgtgagaa ttcttatttt tgttgaagat catcggagtt
    11941 ggcgatccga atcccttgag ttcattagtc cagaaattct tagcggattc tttatccgta
    12001 gtgtttagcc atacaatgaa ttgtttaaaa gaaatgggat ttgaaagttc agcagcctta
    12061 ccatttttaa aagattcata aaatgcaaaa acttcttttt gaatgacggg atagctccag
    12121 ccgtccatta agatatgatg aaagctccag ataaatttgt ataactgatc atttaatttt
    12181 atgagagtac atctcatcag aggtgcggac tccatagaaa aactttttga tcggtctgct
    12241 tttatgaatg ttttaaattc agtttcaata tcctctgatt ttagattact ccagttgaga
    12301 ttttcccatg gcaaagaaac agatttattt actatctgta caggtttgga aattccctta
    12361 tactgaaaag aagttctcaa aactccgtgt ctttttacaa cttcattcca ggctttttca
    12421 aaagcactgg cgtcgagttc tccttcaagg tcataacaga actgttctat gtatgcatca
    12481 gatccttcct catacaaagt atgaaaaagc attccctgct gcatgggaga aagttcgtaa
    12541 atatcttcta ttttattttt tagatcctga tccatttgtt agtttaaatt tgcaagtaaa
    12601 tcatcgagct cctgctgatc cagtccggct gcagaaaagt cagacggagt aaaacctgaa
    12661 ttcttatctt cgcctgtgca atgtgaaata attccattca tggaattttt aaaatgatca
    12721 gcaagctttt ctatattttt tctctgataa atattgttac tgaaattaaa ttcgagtaat
    12781 aacttatcat ctgttaccac tgcatttatt tcaattgaat gaaatctttt attctctcca
    12841 tcctgactaa gctttaatcc ttccacaatt ttccaatcag ttggtaaaaa ctgaaaattc
    12901 atttgcccca gataattgaa tacaacctcc acttcaggaa aatccgaaag tgatttttta
    12961 atttcagtat catccgacat atatttcagt aaaccaaatc caattccgtt attcggtata
    13021 ttcctcagat tctctttgac agatttgatg cattctccgg tatctttaga tgaattgatt
    13081 tttaagagca ctggaaaaac ggaagtaaac cagccgacag ttcttgaaat atctgtacta
    13141 aatgaattat cagcaatatc ttcacgcccg tgaccttcta aattcagaag taatttattc
    13201 tcgccattcc atttattaaa tgccaaaatc aaagctgtta gtaaaatgtc gttgatctgt
    13261 gtattgtaag acttatgtac atcttttaaa atcagtccgg tatcttcttt tgaaaattca
    13321 atgttcacat tttcttcaga ggttacaatg tttt
//
LOCUS       DAORVQ010000090        13130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2230_length_13130_cov_5.136597, whole genome shotgun sequence.
ACCESSION   DAORVQ010000090 DAORVQ010000000
VERSION     DAORVQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_13130_cov_5.136597"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLX80_08525"
     CDS             complement(<1..882)
                     /locus_tag="PLX80_08525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20871.1"
                     /translation="MSADKLSITVLMTVYNDAAYLRQSVNCILHQTFSDFEFLIIDDG
                     STDSSAEIVRSFKDPRIVYKKIKHSGLAAALNYGMKIAAGDWIARSDADDLSVTSRLE
                     IQTDHLLKHPETDVLGSWSVYFSDPSQILFRINPPTEDKKIKEFMELHNPINHSSVIF
                     RKDKILLAGGYDEDFGVYEDFELWLRMKNKLSFNIIPESLVYTRYRKNSMTSLGSRGK
                     IHSLLVKNLLTKSDSDKLFKPGKKDLNIDLSENIKMKLFWIEYFYGSKTRSRDLLKDT
                     NKFRTKDIRKSLAFYNSY"
     gene            complement(864..2030)
                     /locus_tag="PLX80_08530"
     CDS             complement(864..2030)
                     /locus_tag="PLX80_08530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20872.1"
                     /translation="MISEFILPNFLRMYYSPAIILNDMGKIKILFLTGDLNYTDGVSS
                     HLLNLMTELTKDDELELHLMYSGGQALEKFKNTKVRISLNKNLSHDSRSFINFSKAFF
                     EVRKYLKDHEINITHSHNHYAANIAGRAAKNLWINTVQTIHGIIPDIGKLKHFYAGKF
                     ILLNKKNHDYFIRKNIAEEKDIYNVRQGIPENLYSEKKLFRSEPDGNIPKILFASRLI
                     DGKGADIFIKASAIVRKNFTGKLRFVMAGSGEMENDLIKLSTELNTEIEFAGSVEDIT
                     PLMKSADIFVNATLMQSEGFPMSVAEAAFSGCLIISSKAEWLEEVFTDRQDGFTFEKN
                     DYKNLSEKILTALQDRDQSQNFAHTFQTKAKQLFCISEFAEKHKIIYKECLQIN"
     gene            complement(1963..3588)
                     /locus_tag="PLX80_08535"
     CDS             complement(1963..3588)
                     /locus_tag="PLX80_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20873.1"
                     /translation="MHKVKIQKAYFPLIISLSVVIIITALVFFLSFQNTDGIFVYSLD
                     DAYIHLAYAKNIFLHNVWGITSHGFTSTTSSPLWTLLITAVFFLTGINIYIPFIFNVL
                     ISLALVLVIFRILNDQKLPAFYTGAVLIFIIIFTPFSIHIFSGMEHLLHTLLAVMFIN
                     IAAVEFAGRDRSFTFITPAKLSLTVLSFLMMMVRYESIFLIFIAVILFTIKKELRLVI
                     SMVLSGILGSSIFGIKSVLNGRSFFPDSVMLKNNLYMTVTDILGGEPVQKIMTFLDMN
                     KKFIVLFILTVILLFIVIKKHKEFRNYISSSLIILLSTAVLQKLFVSSTFFRYDSYLL
                     ISFICFDAISVNYILKGDPKIYRMLTAPFKSALAILALTIMILYFSYKTFYIDKTVTA
                     VKNIYEQQYQMAAFVNEFYTGSSVALNDIGAVNFYADIYCTDLIGLGSPEIADARLNN
                     NFNKEFITYLSRKNNVKIAIIYDEWFDHDVLIPDEWKPAGKWKITDNFICGSDEITFY
                     ATSQEAYGELINDLRIYSAKLPPDVLQSGDYTQ"
     gene            complement(3591..4691)
                     /locus_tag="PLX80_08540"
     CDS             complement(3591..4691)
                     /locus_tag="PLX80_08540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20874.1"
                     /translation="MKNPKIVFLGRYNQSEILSGPEKVAKRIFFEHTKVNSSVFIEYF
                     FDGNKYGVFKKLFGSEKVTEINSSPVLKKGIFSLLKFLFFNKPDVIHIITYERFCITA
                     VLYKFFSGVKIIYNVHGIVAYENSILRDTKTMYIKKDEFAESKFFKYSDVYIFLSDQS
                     IQAAENYYQITESKIRMVPNGIDRDFHKVIQPVKDLKKEFKIVFSGDPVRKEKGFDLI
                     KEVFYELNFPFRLYLLSNQKIHNEDFIIYLKPMDTVKFSEFLSESDIVISAAGYEPFS
                     MTVTEAMSSGAVPVVSQETGMSRYIRENENGFIFNTKNPDELRSILTKIYNDPELHFH
                     ISQNAKKIYDQLNWEIVYNDYKNIYTKLFPLK"
     gene            complement(4688..5551)
                     /locus_tag="PLX80_08545"
     CDS             complement(4688..5551)
                     /locus_tag="PLX80_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20875.1"
                     /translation="MFRIIYFGEFLPNTYFAKIGHDIVGYYEFRTYKNGIFYILYFLK
                     SNPQFVLFILLLPFVFKRIKINKVFLMTFSIIALQFFFIIFSGGDWMVQYRFAVVSIP
                     FLSIGIMIMVNELTLSEMVSNSMVKVLIILIFTVSALSLTVADRTIINKEILLWNNLK
                     TESANLKNDIPPGSIAANGSSGIIPYYLSEVTFIDIVGLTDKHIAKEGYRHGTWFERS
                     LPEYVYSLDPEWLIMWKKKNADGIFTFEDASPCYIDMAENENFSKYEFYKDYNLYSDV
                     KIELYKKIIQP"
     gene            5637..6080
                     /locus_tag="PLX80_08550"
     CDS             5637..6080
                     /locus_tag="PLX80_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20876.1"
                     /translation="MKPTEKITPSGLETERKNIKKVKGKLYILSEFNLLTIKKQRSKM
                     KMNNISSNPDTAHKINGRLKSIRQTAGRNNPDIPDSLQILKRQMRLKRTKISPVILAV
                     NSRGRPITLNKESNINQLKLENPSTCTPLLNTIPSPFVKLFTYRK"
     gene            complement(6199..6858)
                     /locus_tag="PLX80_08555"
     CDS             complement(6199..6858)
                     /locus_tag="PLX80_08555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20877.1"
                     /translation="MGTSNWQNISYNIDLVKKLNPESILDVGVGFGRWGILFREFLEI
                     WGDRNYSGEWKRKIDGVEIFPLYLKDYHKYFYNNIYETDALGFLENTDNKYDLINCGD
                     VIEHFEKKDALKLISIALEKGRYVLINIPIGNNWEQSDINDNEYERHRSSWEISDFTL
                     YENHLITEFEDVELRRYCVVLLSNEKIDLKKTYGKYFTLKSILKNKLGLGAVVKLIEN
                     R"
     gene            complement(6862..8097)
                     /locus_tag="PLX80_08560"
     CDS             complement(6862..8097)
                     /locus_tag="PLX80_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQY20878.1"
                     /translation="MKINFFKLFRENKYPVYINILERAFFFIVFLIIARVYPSDSYGQ
                     LITIFSLANIFIIIFDLGIPLLIQKELSSGNKVSNNFTGNAIAISIALFPVYLFLLWT
                     YQILFFPEFPFWLFASTAVLVYLFSFSNLLNKALSGIRNFYSQYRALLYSRVLTLCAI
                     LIFSLLFNTDLTFLILLLTVGAIIHITGLLISLKNNNVCINPLTARFKIASRILKMSV
                     PLGLAVLFNFLYDKIDIILISKLTDFENVAFYSIGYSVYKSSSLLYSFLFITGLSRIS
                     YLSKNKKAVRLFYKKYLFILLPVCLVSSLILYFFGGILISILYTDKFSGSEIILRILS
                     FAVIGLALNNLTGVILNGLGMFRQNMNVTLAGLILNILLNIFFIPVYGIAGAAVITVF
                     TEYFIFAGDYLYLKKFKIL"
     gene            complement(8165..8635)
                     /locus_tag="PLX80_08565"
     CDS             complement(8165..8635)
                     /locus_tag="PLX80_08565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY20879.1"
                     /translation="MDKKNINDVKLGKDVKIFDFVNLYGCEIGDNSKVGTFVEIQKNA
                     SVGKNCKISSHTFICEGVHIGDNVFVGHNVTFINDKYPRATAAGGQLQTEEDWKVVEI
                     YIKDGVSIGSSATIMCGVTIGENSIIAAGAVVTKDIPANVIAGGVPAKIMKKVN"
     gene            complement(8652..10088)
                     /locus_tag="PLX80_08570"
     CDS             complement(8652..10088)
                     /locus_tag="PLX80_08570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQY20880.1"
                     /translation="MKYPRYKYIFAFFDLAVIFLSFKLSEYITANVLNKNVILNENEF
                     LTYLTFAVFSLIFVFIFQNNNLYKINILLTRAPHLTALLKSFLYGILLLIIFSFIIKF
                     NLLLSSRFFVLFFFIIGFTGVSFFRILILKPFYMHFTKILNNSNILIIGAGKSGKLLA
                     EKLLFENFYGIKITGFLDDKVEKGEEVFKEFVCFGKITELKQVVKNFEVDEIIISIDN
                     IGYESLMELIDRCNELYKSVKLTSELFNIVPEKIVTESYSGIPVVDLSPKVNRNLNYM
                     YKRVFDYAASFIGLLVLSPLFLIIILLVKYSSKGEIFFKQIRIGKDGNKFTFYKFRSM
                     YIDNVEDTAREQSMMEFMKNDKTMFKGSAKILNEKRVTPIGRFLRKTSLDELPQLFNV
                     LKGDMSLVGPRPCLPYEFDAYDEWHKRRHIVYPGCTGVWQVSGRSSVSFRDSIVLDLY
                     YINNMTPWLDLQLIFKTFPVMIFGRGAK"
     gene            complement(10129..11234)
                     /locus_tag="PLX80_08575"
                     /pseudo
     CDS             complement(10129..11234)
                     /locus_tag="PLX80_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            complement(11245..12018)
                     /locus_tag="PLX80_08580"
     CDS             complement(11245..12018)
                     /locus_tag="PLX80_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HQY20881.1"
                     /translation="MTEFYFKGVKVNCCDLESSVRYCFDFLNEDIGSGYICVTDTGNL
                     VNAYKRSPDLKSAINNSLLSLPDGRPLSMFAGLKGIDGIGRVAGPDFMREVFKRSKDR
                     GFKHFFIGDTEEVLERVTGKAVGEFGIKVCGTCSPAFVDNMNNSRANEEIVSQINSSD
                     ADFIWVALGGGKQEIWMMNNYKKLNKGIMIGVGAAFRFYLGDIKRAPLVFQKLGMEWF
                     FRLIQQPGKMGGRYLKTLPLFILYSSSEIFKSRSELLKK"
     gene            complement(12026..>13130)
                     /locus_tag="PLX80_08585"
     CDS             complement(12026..>13130)
                     /locus_tag="PLX80_08585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20882.1"
                     /translation="GSVPPPVHGSSIYLSNLLNSKVKDEFEVFHHDISDHRDIKNLSK
                     LDITNIKLALKSISGMRKFVKEKKPDIVYIPVASNFLPYLRDGLMILTASAFSNAKIV
                     IHLHEGSFFNEVFYKNSIPPVKWFIRHSLSKVNTAIVFSEKLKENFEGKVKNITAFMN
                     GMDLIPADPLKKIHKESEDKFIISFCSNHYESKGIIDILKSAPAILKEFPKTEFHFAG
                     DWPDDERSTKLKADNILSESGIQNNVKFHGVISGKAKEIFFDNTDILLLPTYYPHEGC
                     PLVIIEAFAKGIPVISSNETGAIPEMIEDGVTGMLIDNKNPEQIADAVIKLIKDKDLR
                     QKISIACTDSFQKRFTAEININNIITTFNKALN"
BASE COUNT         4464 a         2276 c         2012 g         4378 t
ORIGIN      
        1 atatgaattg taaaatgcca gagattttct aatatcttta gtgcggaatt tatttgtgtc
       61 tttaagcaaa tctcttgaac gggttttgct gccgtaaaaa tattcgatcc agaataattt
      121 cattttgata ttttctgaga gatcaatgtt taaatctttt tttcccggtt taaataattt
      181 atccgaatct gatttagtca gtaaattctt tactaacaga ctgtgaattt tccccctgct
      241 tccaagtgat gtcatcgaat tttttctgta tctcgtgtaa actaatgatt cgggaataat
      301 attgaaactt aatttattct tcatcctaag ccatagttca aaatcttcat agactccaaa
      361 atcttcatca tatccgccgg caaggagtat cttatctttc ctgaagatca ccgaagaatg
      421 atttatcgga ttatgaagct ccatgaactc tttgatcttc ttatcttctg tcggaggatt
      481 tatcctgaat aaaatttggc tcgggtcaga aaaatatact gaccaactgc caagtacatc
      541 tgtttcagga tgtttcaata aatggtcagt ctgtatttca agtctgctgg ttacgctaag
      601 atcatcggca tcagatctgg cgatccagtc acctgctgct attttcattc cgtaattcaa
      661 agctgctgct agtcctgaat gctttatttt tttgtaaaca attcttggat ctttaaatga
      721 tcttacaatt tcagcagagc tgtcagtaga tccgtcatca ataattagaa attcaaaatc
      781 tgaaaaagtc tgatgcagta tgcagttaac agactgtctg agatatgcag cgtcattata
      841 cacagtcatc aaaacagtta tgcttaattt atctgcagac attctttgta aataatttta
      901 tgcttttcgg caaattcaga tatacaaaat aactgcttag ccttagtttg aaatgtatgt
      961 gcaaaatttt gagactgatc tctatcctgc aatgcagtta atattttttc tgatagattt
     1021 ttataatcat ttttctcaaa agtaaatccg tcttgtctgt ctgtaaaaac ttcttccagc
     1081 cattcagcct tagaagaaat tataagacaa ccactgaatg cagcttcagc tactgacatt
     1141 ggaaatcctt cactttgcat caatgtcgca ttcacaaaaa tatcagcact tttcattagc
     1201 ggagtaatat cttctacact gccggcaaat tcaatttcag tattcagttc tgtggataat
     1261 ttaattagat cattctccat ttctccggac cctgccatta caaatctaag tttcccggta
     1321 aaatttttcc ttactatagc agaagctttt atgaatatat ctgcaccttt cccatctatc
     1381 aatctggaag cgaaaaggat cttcggaata ttaccatcag gttctgatct gaatagtttt
     1441 ttttcagaat acaaattttc aggaatgccc tgcctgacat tgtagatatc tttttcttct
     1501 gcaatatttt tcctgataaa atagtcatga ttttttttat tcaataaaat aaatttaccg
     1561 gcataaaaat gtttgagctt tcctatatca ggtattattc cgtgaattgt ctgcacagta
     1621 ttgatccata aattctttgc agctctgcct gctatgttag cagcataatg attatgggaa
     1681 tgagtaatat ttatttcgtg atctttcaaa tattttctga cttcaaaaaa tgctttggaa
     1741 aaattaataa aacttctgga atcatgactg agatttttat tcaaagatat tctcacttta
     1801 gtatttttaa atttttccag tgcctgtccg ccggaataca tgagatgaag ttccagttca
     1861 tcatctttgg tgagttcagt cataaggttc agcaaatgac ttgaaacccc gtcggtataa
     1921 ttcagatctc ctgtcagaaa taatattttt attttaccca tatcattgag tataatcgcc
     1981 ggactgtaat acatccggag gaagtttggc agaataaatt ctgagatcat ttatcagttc
     2041 accgtaggct tcctgtgaag tcgcataaaa tgttatttca tcactgccgc agataaagtt
     2101 atcagttatt ttccattttc cggcaggctt ccattcatcc ggtattaaaa catcatgatc
     2161 aaaccattca tcatagatta ttgcaatttt gacattattt ttacggctca gataagttat
     2221 aaattctttg ttgaaattat tatttaacct tgcatcagca atctcagggc tgccgagacc
     2281 gatcaggtcg gtacaataaa tatctgcata aaaattaaca gcaccaatat catttaaagc
     2341 gactgagctg ccggtataaa actcattcac aaacgctgcc atctgatact gctgttcata
     2401 aatattttta acagctgtaa cagtcttatc aatataaaat gttttgtaag aaaaatatag
     2461 aatcattata gtaagagcca aaattgccaa tgcactttta aaaggagctg taagcatacg
     2521 gtagatcttt ggatcgcctt tgagaatgta atttaccgaa attgcatcaa agcagataaa
     2581 agaaattaaa agataactgt cgtacctgaa aaatgtagat gacacaaaga gtttttgcag
     2641 gacagcagtg cttagaagaa tgatcaaaga agatgaaata taatttctga attctttatg
     2701 ctttttgatc actatgaata aaaggatgac cgtcagaata aaaagaacaa taaacttttt
     2761 attcatatct aaaaatgtca tgatcttctg aacaggttca ccgccgagta tatcagttac
     2821 agtcatataa agattattct tcagcattac agagtcagga aaaaaacttc ttccattcag
     2881 tactgatttt attccaaaga ttgaactacc caagatccca gacaatacca ttgaaataac
     2941 taatcgtaat tcctttttaa tggtaaaaag tataacggca ataaaaatta agaatatact
     3001 ttcatatctt accatcatca ttaaaaaact taatacggtc agacttaatt ttgcaggggt
     3061 gataaaagta aaagacctgt ctctgcctgc aaattcaacg gctgcaatat ttatgaacat
     3121 aacagcaagc aaagtatgta acagatgttc cattcccgaa aaaatgtgaa tggaaaatgg
     3181 agtaaaaatt attataaaaa tcagtacagc tccagtgtaa aaagccggca gtttctgatc
     3241 gttgagaatt ctgaaaatca ccaggaccaa agcaagtgaa atcagtacat taaagatgaa
     3301 tggaatgtaa atgtttattc ctgtcagaaa aaaaacagca gttataagta atgtccataa
     3361 tggagatgaa gtagtagaag taaatccatg acttgttatt ccccatacat tatggagaaa
     3421 gatgttttta gcataagcta aatgaatgta agcatcatca agagaataga cgaatattcc
     3481 gtcagtgttc tgaaagctga ggaaaaatac caaagcagta ataattatta caacggacag
     3541 agaaataatt aatggaaaat aagctttctg aatttttact ttatgcatcc ttatttaagc
     3601 ggaaaaagtt ttgtataaat attcttatag tcattgtaaa caatttccca attaagttga
     3661 tcatatattt tttttgcatt ctgtgatata tggaaatgta attccggatc attataaatc
     3721 tttgtcagaa ttgatcttaa ttcatcagga ttctttgtat taaaaataaa tccattttca
     3781 ttttccctga tatatcttga cattccggtc tcttgtgata ctacaggtac agctccgctt
     3841 gacattgctt cggttacagt catcgaaaac ggttcataac cggctgcaga aataacaatg
     3901 tcagattctg atagaaattc tgaaaactta actgtatcca ttggcttaag atatattata
     3961 aagtcttcat tgtgaatttt ctgattgctc aataaataca atctgaatgg aaaattcaat
     4021 tcatagaaaa cttctttaat aaggtcaaag cctttttctt ttctaaccgg atcgcctgaa
     4081 aatacaattt taaattcttt ttttaaatct ttgacaggtt gtattacttt atgaaaatct
     4141 ctgtcaattc catttggaac cattcttatc ttagactcag tgatctgata ataattttct
     4201 gctgcctgaa tagactggtc tgatagaaat atatacacgt cagaatattt gaaaaattta
     4261 ctttcagcaa attcatcttt ttttatatac atagtttttg tgtctctaag aattgaattt
     4321 tcataagcga caattccatg gacattataa ataatcttaa ctcctgaaaa aaatttatat
     4381 agaactgcag taatacaaaa cctttcatat gtaataatat gaataacatc cggcttattg
     4441 aaaaaaagaa atttcagaag actaaagata ccttttttca aaacaggaga cgaatttatt
     4501 tcagtaactt tttcactgcc gaataatttt ttaaaaacac cgtacttatt tccatcaaaa
     4561 aaatattcta tgaagacaga tgaattcact tttgtatgtt caaagaaaat tctctttgcc
     4621 actttttccg gacctgaaag gatctccgac tgattatatc tgcctaaaaa aactatcttt
     4681 ggatttttca aggttgtata atttttttat acaattcaat tttgacatca ctgtacagat
     4741 tgtaatcttt ataaaattca tattttgaaa aattttcatt ctctgccata tcaatataac
     4801 atggacttgc atcttcaaaa gtaaatatac catcggcatt tttctttttc cacatgatga
     4861 gccattctgg atcgagagaa tatacatatt cgggaagtga tctttcaaac catgtaccgt
     4921 gtctgtaacc ttcctttgca atatgtttat ctgtaagtcc gacaatatct atgaatgtga
     4981 cttcactaag ataatatgga ataatacctg aagatccgtt tgctgcaatg cttccgggcg
     5041 gtatatcatt ttttaaattt gcagattcgg ttttcagatt attccataat aatatttctt
     5101 tattaatgat tgttctgtca gcgacagtga gacttaatgc tgatacagta aaaattaata
     5161 ttattaaaac ttttaccatt gagttgctga ccatttctga caaagtaagt tcattgacca
     5221 taatcataat accaatagat agaaagggga ttgagacaac agcaaatctg tactgcacca
     5281 tccagtcgcc tcctgagaaa attataaaaa agaactgaag tgcgatgatc gaaaatgtca
     5341 ttaaaaaaac tttgtttatc tttattcttt tgaaaacaaa cggtaacaaa agtataaata
     5401 aaacaaactg cggattggat ttcaaaaaat ataaaatata aaatattccg ttcttgtaag
     5461 ttcgaaattc ataataccct acaatgtcat gaccgatctt tgcaaaataa gtattaggta
     5521 aaaattctcc aaagtaaatt atcctgaaca ccagaaatga tgagaataag atcaggtatc
     5581 ctgctgtatg ttttaacgaa agggatttgc tgccggaaaa aatataaata aaaataatga
     5641 agccaacaga aaagatcacc ccttcgggtc ttgaaacaga gagaaagaat attaaaaaag
     5701 ttaaggggaa attatatata ctttctgaat ttaatctgtt gaccattaaa aaacaaagaa
     5761 gcaaaatgaa aatgaacaac ataagttcaa atccggatac agcccataaa ataaatggaa
     5821 gattaaaaag cataagacaa actgccggca gaaataatcc tgacattcca gactcattac
     5881 aaattttgaa aaggcagatg agacttaaaa gaacgaaaat tagtccggtg attttagctg
     5941 tcaattcaag aggcaggccg ataacattga ataaggaaag taacataaac cagctaaagc
     6001 ttgagaatcc ttctacctgc acacctttgt taaatactat cccttctccg tttgtgaaat
     6061 tgtttacata ccgaaaatag atatataagt catcgattat ccagtcaaag acatttaaac
     6121 aaagaatcag aaatattata aaaaaaatac ttccgaaaag aatatttttt gattttattt
     6181 tatctgttaa acctgttatc acctgttttc aattaactta acgacagcac cgagtccgag
     6241 tttatttttc aaaatacttt ttaatgtaaa atattttccg taagtttttt tcagatcaat
     6301 cttttcattg gagagtaaaa cgacacagta tcttcgaagc tctacatctt cgaattctgt
     6361 gatcagatga ttttcgtaaa gggtgaaatc tgaaatttcc caggagcttc tgtgcctttc
     6421 atattcgtta tcgttaatgt cagactgctc ccagttattt ccaatgggaa tatttattaa
     6481 aacatatctt cccttttcta aagcaattga aattaatttc aaagcatcct ttttttcaaa
     6541 atgctctatc acatctccgc agttaataag gtcatatttg ttgtctgtat tttctaaaaa
     6601 gccgagggca tcagtttcat aaatgttatt ataaaagtat ttgtgatagt ctttcaggta
     6661 aagcgggaat atttccacgc cgtcaatttt tcttttccat tcacctgaat aatttctgtc
     6721 accccagatc tccaggaatt ccctgaataa gattccccat cttccgaatc ccactccaac
     6781 gtccagaata gattcaggat ttagtttctt tacaaggtcg atgttgtatg aaatattctg
     6841 ccagtttgaa gtacccatta atcagaggat tttaaatttc tttaaatata aataatcgcc
     6901 tgcaaaaata aaatattcag taaatactgt aattactgct gcaccggcaa ttccatatac
     6961 aggaatgaaa aatatgttca gtaaaatatt caggatcaga ccggctaaag ttacattcat
     7021 attctgtctg aacattccga gcccattcag aataactcct gtaagattat ttaatgcaag
     7081 tcctataact gcaaatgata agatcctcag tataatttct gaacccgaaa atttatcagt
     7141 atataaaata ctgataagga tgccgccaaa aaaatataat attaaagacg aaactaagca
     7201 aacaggtaaa agaatgaaaa ggtatttttt gtaaaaaagt ctgactgctt ttttattttt
     7261 actcagataa gatattctgc ttagacctgt aatgaataaa aaggaatata aaagagaaga
     7321 acttttgtaa acgctgtaac cgatactgta gaatgcaaca ttctcgaaat cagttaattt
     7381 tgaaattaaa ataatatcaa tcttatcgta caaaaaattg aacagcacag ccagtcccag
     7441 aggcacagac attttcagga tccgggatgc aattttaaac cgggcagtaa gtggatttat
     7501 gcagacatta ttattcttca aagagatcaa aagtcctgtg atatggatga tcgctcctac
     7561 tgtcaggagt aatatgagaa atgtcagatc ggtgttgaac aaaagtgaaa aaattaatat
     7621 tgcacaaagt gtcaatactc ttgaatataa cagtgctctg tattgtgaat aaaaatttct
     7681 gatacctgag agagctttat taagtaaatt agaaaatgaa aatagataaa ctaaaacagc
     7741 agtggatgca aacagccaga atggaaattc agggaagaat aaaatttggt aagtccataa
     7801 taaaaacaag taaaccggaa ataatgctat acttattgct attgcattcc ctgtaaaatt
     7861 attactgacc ttatttccgg atgaaagttc tttttgaatg agcaggggaa tgcccaggtc
     7921 aaaaattata atgaaaatat ttgcgagtga gaatattgta attagctgac cgtaggaatc
     7981 tgaaggataa actcttgcaa ttatcagaaa gacaataaag aagaaagctc tttcaagaat
     8041 gttaatgtaa accggatatt tattttcccg gaataattta aaaaaattta tcttcaaaaa
     8101 aataactgat taaaaaaaca aattacggga ttcagatgtg tttatataaa aatgtatagt
     8161 aacactaatt tacctttttc atgatcttag cgggaacacc tcctgcgatg acatttgcag
     8221 gtatatcttt tgtcacaaca gcacctgctg ctataataga attttcaccg atcgtaactc
     8281 cgcacataat agtagcagaa gaaccaattg atactccgtc tttgatatag atctcaacga
     8341 ctttccagtc ttcttcagtc tgcagctgac cgcctgctgc agttgcccgc ggatatttat
     8401 cattaataaa agtgacatta tgaccgacga agacattgtc tccgatatga actccttcac
     8461 aaataaaagt gtgggaagat attttacaat tttttcctac acttgcattc ttttgaattt
     8521 ctacaaatgt ccctacttta ctgttatcac ctatctcaca gccataaaga tttacaaagt
     8581 caaatatctt tacatcttta ccgagcttta catcattaat attttttttg tccattgaaa
     8641 ttattatgga attattttgc tcctcttccg aaaatcatta caggaaaggt tttaaaaatc
     8701 aattgcagat caagccaagg tgtcatatta tttatataat acagatccag tacaatcgaa
     8761 tctctgaacg aaactgaact cctgcctgaa acctgccaga caccggtaca gcccggatag
     8821 acaatatgtc ttcttttgtg ccattcatca taagcatcaa attcgtaagg gagacaaggt
     8881 ctcggaccta ctaaactcat gtcacctttc aatacattaa aaagctgagg gagttcgtcc
     8941 aaagatgttt ttcttagaaa cctgcctata ggagttactc ttttttcatt taagatcttt
     9001 gcagaacctt tgaacatagt cttatcattt ttcataaatt ccatcatact ttgttccctt
     9061 gcagtatctt caacattatc tatatacata gatctgaatt tatagaaagt gaatttattt
     9121 ccatctttac caattcttat ctgtttgaaa aaaatttcac ctttggaaga atattttaca
     9181 agtaaaataa ttattaaaaa cagcggtgat aagacaagca gtccaataaa tgaagcagca
     9241 taatcaaaga ctcttttata catatagttc aggtttctgt ttacctttgg tgaaaggtca
     9301 actacaggaa ttcctgagta ggattcggtg acgatctttt ccggaacaat attaaaaagt
     9361 tcggaggtaa gctttacgct tttgtatagt tcgttgcatc tgtcaattaa ttccatcaga
     9421 ctttcgtatc caatattgtc tatagaaatt ataatttcat ctacttcaaa atttttaaca
     9481 acctgtttca gctctgtgat ttttccgaag caaacaaatt ctttaaagac ctcttcacct
     9541 ttttctactt tatcatcaag aaatcctgtg atctttatac cgtaaaaatt ttcgaataat
     9601 aatttctctg caagaagttt acctgatttt cctgcaccta taataagaat attactgtta
     9661 ttcagaatct ttgtaaagtg catataaaat ggtttcagta taagtatcct gaaaaatgat
     9721 actcctgtga atccgataat aaaaaagaat aaaacgaaaa atctggaaga aagcagaagg
     9781 ttaaatttta ttataaaaga aaaaatgatc agaagaagta tgccgtataa aaatgatttg
     9841 agtagtgcag tcagatgagg agcacgtgtc aggagaatgt tgatcttgta taaattgttg
     9901 ttttgaaaaa tgaagacaaa tatcagagag aatactgcaa atgtaagata agtaagaaat
     9961 tcattttcgt ttaaaattac atttttattc agaacgtttg cagtaatgta ttccgataat
    10021 ttaaatgaaa ggaatatcac tgcaagatca aaaaaagcaa aaatatattt atatctggga
    10081 tacttcatta ttgttcagat attaatgtta ctacaagaat aactcaattt atccttttat
    10141 gaaatgttta attttatcac acacaaattc aatttgatca tcagtaatat caggaaacat
    10201 tggcagcgac agacaatcag atgcaagttt ttcagttaca gggaaatatg atgctttata
    10261 atttagatct gcaaaacatt tttgcagatg cagaggaact ggataatgca gaccggtact
    10321 aatctcacaa gaattaagga aattctgcag cttatctctt tcagaatatt gaatgacaaa
    10381 taaatgataa acatgtttgc catattccat ttccagaggc agtttgatca gttcaatatc
    10441 ttttaataat ttacgatatt tatctgcagc tgctcttctt ttttcagtcc atttatcaag
    10501 atgatccagt tttaaattta agacagctcc ttgaattcct tccattctgt aattgtgacc
    10561 atagaattcg tgatgatatt ttttttctga accatgatct ctgagcattc tgatctttct
    10621 tgaaagttca tcatcatttg tcgtaactgc tcctccttca ccgaatgcgc cgagattttt
    10681 cccgggataa aaactaaaac tggcaacaga agaaagactt ccggctttct ttcctttgta
    10741 ttctgcaaga tgcgcctgag cagcatcttc tattaaaaga atattatgtc tgtcagaaat
    10801 atcttttaac gcatccatat cagcagtctg tccatataaa tgaactgcaa ttatggcttg
    10861 gttttttcag taatcatatt ttcaattttt accggatcga gattgtaact ttcaggatgg
    10921 caatctgcaa atacaggtgt agctccgcaa agagttgctc cccatgcagt tgctataaaa
    10981 gtatttgctg gcattatcac ttcatcacca tgactaatac cgtgagccca gagtgcgaga
    11041 tgatttgcat cagtgcctga acttacagca atgcagtgtt tagaatcatg agctttagca
    11101 aaagcttctt caaaatcaga aactgcttta ccaagtatga atgaagaatt ttctataata
    11161 tttgtaatgg aagaaattgc ttcttctttg ataagatcgt tttgcatttt gagatctgtg
    11221 aaaggtacct gcattaaatt aaatttattt ttttaataat tcggatcttg atttgaatat
    11281 ttcgcttgaa gaatataaaa tgaataatgg taatgtcttt aaatatctgc cgcccatttt
    11341 tcctggctgc tgaattagtc tgaagaacca ttccatgccg agtttttgaa ataccagagg
    11401 agctcttttt atatcaccta agtaaaatct gaaagcagca ccgactccaa tcattatacc
    11461 tttgttaagt tttttataat tattcatcat ccatatttcc tgtttaccgc caccaagtgc
    11521 tacccagatg aaatccgcat ctgatgaatt aatctgactt acaatttctt cattagctct
    11581 actgttattc atattgtcaa caaaggcggg actacaagtt ccgcaaacct ttatcccaaa
    11641 ctcaccaaca gccttccctg ttactctctc caaaacttcc tctgtatctc caataaaaaa
    11701 atgtttaaag cctctatcct tacttctctt aaatacttct ctcataaaat caggacctgc
    11761 cactctgcca attccatcta tacctttaag acctgcaaac attgacaaag gtctgccgtc
    11821 aggtaacgat agcaaagaat tatttattgc tgattttaag tcaggtgaac gtttgtaagc
    11881 attgacaaga tttccggtat cggttacaca gatatatcct gaacctatat cttcattcag
    11941 aaaatcaaaa caatatctga ctgaactttc cagatcacag cagtttactt ttacaccttt
    12001 aaaataaaat tctgtcaaat atatatcagt ttaatgcttt attaaatgta gtaataatat
    12061 tattgatatt aatttcagct gtaaatcttt tttgaaaaga gtctgtacag gcaatactta
    12121 ttttttgtct aagatcttta tctttgataa gtttgattac agcatcagca atctgctcgg
    12181 gatttttatt atctattaac atgcctgtca caccgtcttc gatcatttca ggaatagcgc
    12241 cggtttcatt tgaagaaatt accggaatac cttttgcgaa agcttcaatg ataacaagcg
    12301 ggcagccttc atgagggtaa tatgttggta ataaaagtat gtcagtattg tcaaaaaaaa
    12361 tctcctttgc ttttcctgaa atgacgccat gaaatttaac attattttgt attccgcttt
    12421 cggataaaat attatcagct tttaatttag tagatctttc atcatcgggc cagtcacctg
    12481 caaagtgaaa ttcagttttt ggaaattcct ttaaaatggc aggagcagat tttagaatgt
    12541 ctattatacc tttgctttca taatgatttg aacagaagct tatgataaat ttatcttcag
    12601 attctttatg aatttttttc agaggatctg caggaataag atccattcca ttcataaaag
    12661 cagtaatgtt tttcactttc ccttcgaaat tttctttcag cttttcacta aagacaattg
    12721 ccgtatttac ttttgataaa gaatgtctga taaaccattt tacaggcgga atagaatttt
    12781 tataaaatac ttcattgaaa aaactgcctt catgaagatg aataactatc tttgcatttg
    12841 aaaatgccga tgcagtcagg atcataagcc catcacgaag ataaggcaga aagttggaag
    12901 caacaggtat ataaacaata tcgggctttt tctctttgac gaactttctc attcccgaaa
    12961 tactttttaa agctaattta atatttgtta tatcaagttt agaaagattc tttatatctc
    13021 tgtgatctga aatatcatga tgaaagacct caaattcatc tttgacctta gaattcagaa
    13081 gatttgaaag ataaatactc gagccatgaa caggcggagg aacacttcct
//
LOCUS       DAORVQ010000091        12986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2272_length_12986_cov_5.115614, whole genome shotgun sequence.
ACCESSION   DAORVQ010000091 DAORVQ010000000
VERSION     DAORVQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_12986_cov_5.115614"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..792)
                     /locus_tag="PLX80_08590"
     CDS             complement(139..792)
                     /locus_tag="PLX80_08590"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HQY20883.1"
                     /translation="MIIKLLVTTSILFSFLVSKNNNSDSIKLKVFEISDSTITNGKKF
                     KTIQPGVIEQGREIKFDNANLAPQNVRVIFNDFFKKYIAVKEALVYKDSYGAGSKTLT
                     LLEQMKSKSDEVNKDIKDARWDIFMHNYENIVKKVKDNKFIAEQRFTFSEVSRGLENF
                     IKQYGLYDKTIYLMQYRNDSLNINSTWMTDKKDFKNPYTGELNDTVYSKVKEVWVFE"
     gene            994..1737
                     /locus_tag="PLX80_08595"
     CDS             994..1737
                     /locus_tag="PLX80_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HQY20884.1"
                     /translation="MSKTFLSAEWRKLIMINYSLDKKDKKDKKILEPFIPYNTELDLW
                     NDKCYISLVGFMFLNTRLKNIKIPFHRNFEEVNLRFYVRHFNKENNEWRRGVVFIKEI
                     VSKRALSFIANNLYKENYETMPMSHNWESDQNAGELSVEFKWKKYEDWNSIKVIAGTE
                     LSKIEKESEAEFITEHYWGYTKINESSTSEYGVTHPRWEIYKTIEYSINVNFENTYGN
                     EFGFLNSETPASVFLAEGSEIEVKEGRKI"
     gene            complement(1749..2972)
                     /locus_tag="PLX80_08600"
     CDS             complement(1749..2972)
                     /locus_tag="PLX80_08600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20885.1"
                     /translation="MKILFLANRFPYPPYRGDKLKIFNLAKQLSERHTLYLITFIDNK
                     DDYNYLPELEKYFDEIKLVYMPHFMSAFNSLSNVFSETPFQINYFRSNRLKKVLEEFI
                     SQNEIDVIHTQHLRMAQYTAGLKNVSTVLDLPDAYSMYWERRASQKKNPLINFMERSE
                     YKKVLKYEKIICEFDLTLVCSEEDKNYLIAEHELNNIEVLENGVDTDQFGGEEHDYFI
                     DDQIIFTGNMTYHPNIDAAVYFAEEIFPEVLKKFPNVKFKIAGQNPTEKILKLQSENI
                     IVTGFVKSLSDEYSKSSIAVSPVRVGAGSMNKVLEPMSMGVPVVSSEIGFKGLGAKAG
                     LDILLAENKSEFINSVCNLLSHAEYRRYIGMSGREVIQNNLSWEKIALKLEDYFYSVA
                     DHRKEEYIIHFENAV"
     gene            complement(3000..3809)
                     /locus_tag="PLX80_08605"
     CDS             complement(3000..3809)
                     /locus_tag="PLX80_08605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20886.1"
                     /translation="MYDLQIQIINYNTKRYLEVCLDGLFKDLKDSGLKYKVLVLDNNS
                     DDDLSDTEEKYSGEEISFSSSDKNLGFGGGHNFLSKKTDSKFVLILNSDIKFIQPDTV
                     KNLYERLISKSEFSVAGPKLLLEDLSCQEFDHGELHGIYSWMKNNYGSSYWKPRDSEA
                     EAAWVSGAVFLCDKKVFEKVNGFDEKFFLYKEEEDLCKRIREDGFKILYFPEVRVLHI
                     GHVVAKRSIHFSDSMDHFLNKHYRKNMSYKILDTIKKIKDISLYGKVRQRS"
     gene            complement(3757..5031)
                     /locus_tag="PLX80_08610"
     CDS             complement(3757..5031)
                     /locus_tag="PLX80_08610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20887.1"
                     /translation="MKILFISYDFPYPPTGGSISRDYNLIKQLSKKHELHWINRTTRG
                     KISQDRIDEMGKYFEEMEIIEWDYEHSITGLLKSLFTDTPYIIRRFYSEQMKELVTKS
                     IAKNNFDLILCDHIYLAQYIPDSVIGKIPVIPNNEDCGFTFYKRMAEESVFIRRIYAR
                     SQWKKLLKYEIEILKKFKVYITTSEKEKELIFSYYNEAEIKVIENGVNTDYFYKRENT
                     VKDPTVIFTAWFGYYPNTEAAIYFAKKIFPLIKKEIPELKFIIAGKEPPKSVIELSSV
                     EGVLVTGYVDDIRDFLCKASAAVIPLKVGGGTRLKILEAFSMGVPVISTELGAEGILA
                     VENKDILIGKNEKDFAEKVVKVLKNTELSEILSENGRALAEKKYDWEIIGDELNKFLC
                     EFVENFNYTNKGNNSNVRSADTNNKLQYEEIS"
     gene            complement(5042..6199)
                     /locus_tag="PLX80_08615"
     CDS             complement(5042..6199)
                     /locus_tag="PLX80_08615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY20888.1"
                     /translation="MPDKKIKILHISPDGKLYGTERHILALVKHSDSNKFEHYVATPE
                     RGNFNDELDKIGVQYFIAGRKHGYKSKLDGITAEGSKELYRIMKKEKFDIVHSHLNSF
                     GGIVGRIAGAGSVVHTRHGVFWSEEELANISVKDKYFQKIKSGIFDLTIAIGEYEKNT
                     MIGKFDYDESKIRVTVNGVDTDELNSKIDRTISKKDLFGTDEFIIGTVGRLERQKGFN
                     MLIEAASLIKDSLENIKFVIIGDGSCKDELKNLRDGFGLQDKIEFIPYKENIPDYVHN
                     FDIYISTSLWEGMSYSVQEAMALGKPVIALTSENVSGLKELIEDGVSGILIEENYIEN
                     LAEKIKLLIKDENQRKFLGENAQEREKNYFQEWRTAEDMDKVYAELNISKI"
     gene            complement(6189..7388)
                     /locus_tag="PLX80_08620"
     CDS             complement(6189..7388)
                     /locus_tag="PLX80_08620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY20889.1"
                     /translation="MKRFLKNIISFVGSIFFFFKRKEIDRSEIKKILVVSLYFKGDVL
                     MNTPAVKFLKKMFPDVEIDIWVKSRSEAVIKNNPDINEVIVFDKIKTADYNDSDKFYV
                     NEKINFLRSVRNKNYDLCIDLTGKYTTALFAMAGGFKYSIGLNYNGFGFCYSKFIDTD
                     TQHTAGHLSEKYLNVIREGLVVSDIEYANLMSEDGIKNVLSIDENSVRKSEEILYAIG
                     IDDNEDYIVIQPTAGWKAKEWSPEKYWRLCGKLSENYKTVLIGSENDRELLEFIKGNS
                     GANDRCTVLLIPLELSAAVISGSVLFIGNDSVGLQIAGAFDVPSIALFGPTNPEFSNP
                     LGEIHKVLYKKLFCSASESEQYCTRNAGKTCPDIKCMNSIQVSEVFAQINLLLKNNSK
                     KSFQIAG"
     gene            complement(7372..8472)
                     /locus_tag="PLX80_08625"
     CDS             complement(7372..8472)
                     /locus_tag="PLX80_08625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY20890.1"
                     /translation="MTINFILPFAGNKPIGGFKIVYEYANRLSEKGHCINVIHPSYTF
                     KESALNNLKYQMRYHQRKIDNSFQPDNWFKLNENVNSIWVRNISDETIPDADVSIATA
                     WRTAHCANGLNKSKGEKFYFIQHYEIWNGSEEEVNETWKMPFKKIVIAKWLEDIAASM
                     NEESYYVPNAFDFSEFGMDILPEKRSAKKIMMLYNELEFKGSKYGLEAFSVLKKEFPD
                     LELNLFGVPDKPDGLPEGVKYFQSPDRQTLRNLYNESSIFVSPSLAEGFPLPPAEAMI
                     CGAALACTDIGGHREYAVNNETAVLFNSEDSNAAADTVRKLIVDDKFRINIANKGNEY
                     IKKFTWENSVNKFQEVLKLHTENKININEKIS"
     gene            complement(9026..10573)
                     /locus_tag="PLX80_08630"
     CDS             complement(9026..10573)
                     /locus_tag="PLX80_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HQY20891.1"
                     /translation="MNSTSRVFSRLFIIVILIKIFDVFKNLVIAAVLGVSSSADIYTA
                     LIMIPDSLIVLIGLDTIKGVVNSEYASLQKDQEKNLIWKSFNNLFNVLFLIGIVVVSI
                     IIIFNSTVIKILLPGFEGEKLLRSIEISYIIFPILFLKIFTGYFHSVYNALKKFFLPV
                     IVPVLTGIALLVSIVMPFYKDDVVYNLSFANLAGNFLMAGVMTYGLVKLGAEFRLRKF
                     DFLKLDEVTKKVLKGSASILLLVICNQIYILSRNYFASFFGEGAVSSLHYSGTLTSVI
                     FSLIFSVFFTVLISDLSTLFAFEKKIKAKNLFLNTFSVLLFMLIPVTVVFLTFGKDIL
                     SMVYLRGNFDQSGIDMTLKPFFWDALSLLTFVMYIVPTALFLAKKEYRLMTKIGSITY
                     LSGILLNYLLSGYFGFYAISMATFITTGIYGLMLLYFSRKIIGRFTEHLKKFLLMFLS
                     GGLTFLIVNILKDYFLNDTLDNEIPALLMRVVICSSLTLLIYLTISSVLKVNFTRQIL
                     KSFKKSS"
     gene            complement(10585..11790)
                     /locus_tag="PLX80_08635"
     CDS             complement(10585..11790)
                     /locus_tag="PLX80_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY20892.1"
                     /translation="MKAHSLEIIRQEDIKLTPELILILTPITFIVGNWYLSALFILSA
                     FAFMMLRYFTFKDHEIKFSNMIPWSLMLLWGIYVCYITPDPVKAVKYYTGTMLLPFFI
                     YILFNNLRLNEKVLTKFFDAMLISGTVIGMYSVYTYIIFNMRSDMRVPSFWNDFNIVS
                     AYMMILFMFNLSFLLKNDNRKKKVFYLFTITFILLGIYLTKTRGVWLAIIFSIAVFFI
                     RKPKIIVPSLLLIGIIILLFYNIVEERVLSVINFEQDNSSLGRLQAWYSTILMIIEKP
                     LLGYGFDTYYLHKYDVMAYYIVEVPHPHNTYLRAIQEMGLIGAVIYLYLFFKAAVYSF
                     KFGGKKSDPVYSKYFDGLQLSFVGLIVAFNFEPYMSLFGVITIVIWILISLTFRLSQS
                     SQSAIISSN"
     gene            complement(11771..12922)
                     /locus_tag="PLX80_08640"
     CDS             complement(11771..12922)
                     /locus_tag="PLX80_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HQY20893.1"
                     /translation="MSDNTEEVVRAPKMEFTLKDFIGIYKEKKKRIFANCLILGILTA
                     VLVYFIIDPIFLSYGTVKTTGKVLGLGMGISVPEISEFGEVLSGSSSSKELALYEDIL
                     LSRRCLEEYIVRFGLMEENDYKNMQKAIKDVRENILYILKDKVSGTMDIGVYDKDPVK
                     SKEMSEFLIAQLDKINIAMNRQNAKNQRDFIQERYEASSAELIIVEDSLKQFQDIYGL
                     SPEIQVKEISELEIKLEAEIESEKIKLELLQKIISPGEDEVQYQRDKINVLERRLFDL
                     QNSTDGSSKLQLKGTPDIVLNFIRLKRNVEIQNKILSVLLPLYEQAKIEEKKEIPTII
                     VLDQPFVPDEKSKPKRLTSIFVVMVLGFIFSYGFFFIKKQLKRIEGTLT"
BASE COUNT         4236 a         2402 c         1857 g         4491 t
ORIGIN      
        1 gtataattta ttttaatttt tataaaaagc ggaatattat atttcgcttt ttattttaga
       61 ttcataaata aattttttga cagtactttg ttgcatcaga gttatttgct cttcggactc
      121 tgcggataat gaaaaaactt actcaaacac ccaaacttct ttcaccttag aataaacagt
      181 atcattcaat tcacctgtat atgggttttt aaaatccttc ttatctgtca tccatgtaga
      241 atttatattc aaagaatcat ttctgtactg catcagataa attgtcttat cataaagtcc
      301 gtactgcttt ataaaatttt ccagtcccct gctgacttca ctaaaggtaa acctctgctc
      361 agcaataaat ttattgtctt ttactttctt gacgatattt tcatagttgt gcataaaaat
      421 atcccatctg gcatctttga tatctttgtt cacttcatca gattttgact tcatttgttc
      481 aagcaaagta agcgtcttac tacctgctcc atacgaatcc ttatagacga gtgcttcttt
      541 aactgcgata tactttttaa aaaaatcatt aaatataact ctgacattct gtggtgctaa
      601 atttgcatta tcaaatttta tctccctacc ctgttcaatt acacccggct gaatagtctt
      661 aaattttttc ccgtttgtaa ttgttgaatc ggatatttca aatactttta atttaatcga
      721 atccgaatta ttattttttg atactaaaaa agaaaataaa attgacgttg ttactaataa
      781 ttttattatc atgtctgaaa attaatttta tatattttat tgtctgaata tgcaaagcag
      841 tataataaat ttaaagtaaa atatgatgat atttttcgat ctcaataaaa cacttcgatt
      901 catgatctaa aagaaaaagt aattagttta cagaatcttg aaactgaatt tactattttc
      961 gattacaaat ttttccaaac atctgattac aatatgtcaa aaacttttct gtctgccgaa
     1021 tggcgaaaac ttataatgat aaattattca ttggataaaa aagataaaaa agataaaaaa
     1081 atacttgagc cgttcattcc ttataatacc gaactcgatc tgtggaacga caaatgttac
     1141 atcagtctcg tcggatttat gtttttaaat acccgtttaa aaaatatcaa aatccccttt
     1201 catagaaatt ttgaagaagt aaatctaaga ttttatgtaa gacattttaa caaagaaaat
     1261 aatgaatgga gacgtggtgt cgtatttata aaagagatcg tctcaaaacg tgctctgtca
     1321 tttattgcaa acaatcttta taaagaaaat tacgagacta tgccaatgtc acataattgg
     1381 gaatccgatc agaatgccgg tgaattatca gttgaattta aatggaaaaa atatgaagat
     1441 tggaattcca taaaagttat tgccggtacg gaattatcaa agattgaaaa agaaagtgaa
     1501 gctgaattca tcacagaaca ttactgggga tatacaaaaa taaatgaaag ttcaacttct
     1561 gaatatggcg ttactcatcc aagatgggaa atatataaga caattgaata ttctattaat
     1621 gtaaattttg aaaacactta tggaaatgag ttcggttttt taaattcaga aacacctgcc
     1681 tcagtcttcc ttgcggaagg ttcagagatc gaagtaaaag aaggcagaaa gatctgagtt
     1741 aagtatattt aaacggcatt ttcaaaatgt ataatatatt cttctttcct gtggtctgca
     1801 actgaataaa aataatcttc gagttttaaa gcaatcttct cccagctcag attattttgg
     1861 atcacttccc tgccggacat tccgatatat cttctgtatt ctgcatgaga taaaagatta
     1921 catacagaat taataaattc agatttattt tctgcaagca aaatatccaa tccggctttt
     1981 gctccaagtc ctttaaaacc tatttcggat gaaactacag gtacacccat tgacatcggc
     2041 tcaagtactt tgttcatcga tcctgcaccg actcttaccg gtgatactgc tatagaactc
     2101 ttactgtatt catccgaaag tgacttcaca aatccggtaa ctataatatt ttctgattga
     2161 agtttaagaa ttttctcagt cggattctga cctgcaattt taaatttcac gtttggaaat
     2221 ttcttcaata cttccgggaa tatttcctct gcaaaataaa ctgccgcatc tatatttgga
     2281 tgataagtca tgttacctgt aaaaattatc tgatcatcaa taaagtagtc atgctcttct
     2341 cctccaaact gatctgtatc aactccattt tctaaaacct caatgttatt caattcatgt
     2401 tcggcaatta aataattttt atcctcttca gaacaaacaa gcgtaagatc aaactcacat
     2461 ataatctttt catatttcaa tactttttta tattctgatc tctccataaa atttatcaat
     2521 ggattctttt tctgcgaagc tcttctttcc cagtacatgg aatatgcatc aggaagatcc
     2581 agtactgtac tgacattttt caatccggca gtatattgag ccatcctcag atgctgtgta
     2641 tgaatgacat caatttcatt ttgtgaaatg aattcttcca aaactttttt gagtctatta
     2701 gatctgaaat aatttatctg aaaaggagtt tctgaaaaaa cattactgag actgttaaaa
     2761 gcagacatga aatgcggcat atatacaagc ttaatttcat cgaaatattt ttcaagttcc
     2821 ggcagataat tataatcatc tttattatct ataaaggtga tcagatataa tgtatgcctt
     2881 tcagataact gttttgccag attaaatatc tttaatttat ctcctcggta tggtggatac
     2941 gggaatcgat ttgcaagaaa taatatcttc attttatatt tgtttaaatt ttttcaaaac
     3001 taacttcttt gtcttacttt tccgtaaagc gatatatctt ttattttttt aatagtatcc
     3061 agaattttat agctcatatt ttttctgtaa tgcttgttca gaaaatgatc catggaatca
     3121 gaaaaatgaa tgcttctttt tgctaccaca tgtccaatgt gtaagactct cacttctgga
     3181 aaatagagta ttttaaatcc atcttctctg atccttttac aaaggtcttc ttcttcttta
     3241 taaaggaaaa acttttcatc aaaaccattt actttctcaa aaactttttt atcacataaa
     3301 aacactgcac ctgaaaccca tgcagcttca gcttcggaat ctcttggctt ccagtatgac
     3361 gagccataat tatttttcat ccatgagtaa attccgtgaa gttccccatg atcaaactcc
     3421 tgacagctaa gatcttccag taaaagcttt ggacctgcta cactgaattc acttttgctt
     3481 attagtcttt catacaaatt ctttacagtg tcaggttgta tgaatttaat atctgaattc
     3541 agaatcaata caaatttgct gtcagttttc tttgaaagaa aattatgccc tccgccaaat
     3601 ccgagatttt tatctgaact cgaaaaagag atctcttctc ctgaatattt ttcttcagta
     3661 tctgaaagat catcatcaga attattatca agcacgagaa ctttatactt cagacccgaa
     3721 tctttcagat ctttaaaaag cccgtccaga caaacctcaa gatatctctt cgtattgtaa
     3781 tttattattt gtatctgcag atcgtacatt tgaattgttt cctttgtttg tataattaaa
     3841 attttctaca aattcacata agaatttatt aagttcatca ccgataattt cccagtcata
     3901 ttttttttca gctaaagctc ttccattttc agaaaggatt tcagaaagct ctgtattttt
     3961 taaaactttt acaaccttct cagcaaaatc cttttcgttt ttaccaatta atatatcttt
     4021 attctcaact gctaaaatcc cttcagcgcc aagctcagta gatattacag gaacacccat
     4081 tgaaaatgct tcaagaattt tgagacgtgt accgcctccg acctttagag gaattacagc
     4141 cgcagatgct ttgcagagaa aatctcttat gtcatcgaca tatccggtta caagaactcc
     4201 ttcaacagat gacagctcta tcacactttt cggcggctcc ttgccagcga ttataaattt
     4261 aagttcagga atttcttttt taatcagtgg aaaaatcttt tttgcaaaat aaattgctgc
     4321 ctcagtattt ggataatatc caaaccatgc agtaaagatc actgtcggat cttttacagt
     4381 attttctctt ttgtaaaaat aatctgtatt cactccattc tcgatcacct ttatttcagc
     4441 ttcattataa taagaaaata taagctcttt ttctttttct gaagtcgtaa tatatacttt
     4501 gaattttttt aaaatttcga tttcgtattt cagtaacttc ttccattgtg aacgcgcata
     4561 aattcttctg ataaataccg actcttcagc cattctttta taaaaagtaa atccgcagtc
     4621 ttcattattt gggatcacag ggatcttacc gatgacgcta tccggaatat actgcgcaag
     4681 ataaatgtga tcacaaagta tcagatcaaa attatttttg gcaatgcttt tagtaactaa
     4741 ttctttcatc tgttctgaat aaaatcttcg aatgatataa ggtgtatctg taaacaaact
     4801 tttcaaaagt cctgttatac tgtgttcata atcccattca attatttcca tttcttcaaa
     4861 atattttccc atttcatcaa tcctgtcctg acttattttt cctctggtgg ttctgttgat
     4921 ccagtgcagt tcatgttttt ttgacaactg ctttataaga ttataatctc tgctgataga
     4981 accgccggtt ggaggatatg gaaaatcata tgatatgaaa agtattttca tgactgcttt
     5041 actaaatttt tgatatattt aattctgcat aaactttatc catgtcctct gcagttctcc
     5101 attcctgaaa ataatttttc tcacgttcct gtgcattctc tccaagaaat ttcctttgat
     5161 tttcatcttt gataagtaat ttaattttct ctgccagatt ttctatataa ttttcctcaa
     5221 ttaatatccc tgaaactcca tcctctatta gctccttcag accgcttaca ttttcagaag
     5281 tcagtgctat caccggcttt cctaatgcca ttgcttcttg cacggaatac gacattccct
     5341 cccatagaga agtggaaata taaatgtcaa aattatgcac atagtctgga atattttctt
     5401 tatatgggat gaattcaatt ttatcctgaa gtccgaatcc gtccctgaga ttcttcagtt
     5461 catccttaca gcttccgtct ccgattatta caaactttat attttcaaga gagtctttga
     5521 tcaaagaagc tgcttcgatc agcatgttaa aacccttttg tctttccagt ctcccgacag
     5581 taccgattat aaattcatca gttccgaaca gatccttttt ggaaatggtc ctgtctattt
     5641 ttgaattaag ttcatcagta tctactccgt tcactgttac ccttatctta ctttcatcat
     5701 aatcaaattt cccgatcata gtattttttt catattcccc tattgcaatt gtcagatcaa
     5761 agatccctga ttttattttc tgaaaatatt tatcttttac agaaatattt gcaagctcct
     5821 cttcactcca gaatacaccg tgtctagtat gcaccacaga acctgcaccg gcgatgcgac
     5881 cgactattcc gccaaaagaa tttaaatgtg aatgtacaat atcaaatttt tcctttttca
     5941 ttattctgta aagttcttta gaaccttcag cagtaattcc atcaagttta cttttatacc
     6001 cgtgtttcct gccggcgata aaatactgta ctccaatttt atcaagctca tcattgaaat
     6061 ttcctctttc aggagttgca acataatgtt caaatttatt ggagtcagaa tgttttacaa
     6121 gcgcaaggat atgcctctct gtgccgtaaa gttttccgtc tggtgagatg tgcagtattt
     6181 taattttttt atccggcaat ctgaaatgat ttttttgaat tattctttaa taataaattt
     6241 atctgtgcaa aaacttcgct tacctgaatg ctattcatac attttatatc cggacaggtc
     6301 tttccggcat ttcgtgtgca gtattgctct gactctgatg ctgaacagaa tagtttttta
     6361 tataatactt tatgaatctc acccaaagga tttgaaaatt caggattggt gggtccgaac
     6421 aatgctatag acggaacatc aaaagcaccg gctatctgca atccgacaga atcatttcca
     6481 ataaataaaa ctgatccgga tattactgct gcgcttagtt ccaatgggat taaaagaact
     6541 gtacatctgt catttgcacc ggaatttcct ttgataaatt caagaagttc cctgtcattt
     6601 tcagaaccga ttaaaactgt tttgtaattt tctgataatt tcccgcaaag tcgccaatat
     6661 ttttcaggtg accattcttt tgccttccat cctgcagttg gctgaatgac tatgtaatct
     6721 tcattatcat cgatcccaat agcatacaaa atttcttctg atttcctgac agaattttcg
     6781 tctatgctaa gtacattttt tattccgtct tcagacataa gattagcata ttcaatatcg
     6841 ctgacaacaa gtccctctct gattacattt aaatatttct ctgaaagatg accggcagta
     6901 tgctgagtat ccgtgtcaat aaatttcgaa tagcaaaatc caaatccgtt ataattcagt
     6961 ccgatggaat atttaaatcc acctgccata gcaaataaag cagttgtata ttttcctgta
     7021 aggtctatac agagatcata atttttattt cttacagatc taaggaaatt aattttttca
     7081 ttaacataaa atttatcact gtcattataa tcagcagttt taattttatc aaaaacgatc
     7141 acttcattta tatccggatt attcttaatg acggcttccg atcttgactt tacccagata
     7201 tcaatttcaa catcaggaaa catcttcttt aaaaatttta cagcaggagt gttcatcaat
     7261 acatcgcctt tgaaataaag tgagaccaca agaatttttt taatctctga tctgtcaatc
     7321 tcctttcttt tgaaaaagaa aaatatgctg ccgacaaatg atattatatt tttaagaaat
     7381 cttttcatta atattaattt tattttcagt atgtaacttg agaacttcct gaaatttatt
     7441 tactgaattt tcccaggtaa attttttgat atattcattt cctttatttg caatatttat
     7501 cctgaactta tcatcaacta ttaattttct gactgtatct gcagcagcat tactgtcttc
     7561 agaattgaag agcacagcag tctcattatt tactgcatat tctctgtgtc ccccaatatc
     7621 agtgcaggca agtgcagcac cacatatcat tgcttctgca ggcggcagtg gaaaaccttc
     7681 tgccagactc ggactaacaa aaatagaaga ttcattatat aagtttctga gagtctgtct
     7741 gtcaggtgac tgaaaatatt taactccttc aggcagtcca tcgggtttat caggcactcc
     7801 aaataaattc aattccagat ccggaaattc ttttttcaaa actgaaaaag cttccagtcc
     7861 gtatttactg cctttaaatt caagttcatt atagagcatc ataatttttt ttgctgatct
     7921 tttctcaggc aaaatatcca ttccaaattc cgagaagtca aatgcattcg gcacataata
     7981 tgattcctca ttcatagatg cagcaatgtc ttccagccat tttgcgatca ctattttttt
     8041 aaatggcatt ttccatgttt catttacttc ctcttcactt ccgttccata tttcataatg
     8101 ctggataaaa taaaattttt ctcccttact tttattcaga ccatttgcgc aatgtgctgt
     8161 cctccatgca gttgcgattg atacatcggc atcaggaatt gtttcatcac tgatatttct
     8221 cacccatatc gaatttacat tttcatttaa tttgaaccag ttatccggct gaaagctgtt
     8281 gtcaattttt ctctgatgat atctcatctg atatttcaga ttatttaatg ctgattcttt
     8341 gaatgtatat gacggatgaa tgacatttat acaatgacct ttttcggaaa gacggtttgc
     8401 atattcatat acgatcttga aaccacctat tggcttattc cctgcgaacg gtaaaataaa
     8461 attgattgtc attataataa tttctcttaa gcaaagataa gaattttata tctttataaa
     8521 aaatttaaat acctgtcaaa cgtaggaatc aaattcaaaa atgaactttt tattcagcct
     8581 gacacgatgg tattgcctca aaggctctaa aattctaaga tcttaaaacc ttaaacactt
     8641 aaaactttta aatcataaaa ctataaaatc tgaaatcata aaacaaaaaa tgtaataact
     8701 tttttaataa ataactttga gccttcgaga ctttgaggtt ttaaggcaaa ttcaatttaa
     8761 tgttctcatc ctgatttacc caaaaacatg agtcacaaat aaattaaaat tgctcctccc
     8821 ttttatcaaa ggggaggctt ggaggggttt gtaaaattaa ttttaacctc gttgtaaacg
     8881 ggcgacaacc tccttcgcac ccactttttt aaggggaatt aattataaat tattctggat
     8941 cgtgctattg aacttttaga tttcatttct aaaaaattga tataaaattt atattcaatt
     9001 ctgcggttaa ataaagttgc attgattaac tacttttttt gaaagacttc aaaatctgtc
     9061 gggtgaaatt cacttttaat acggaagaaa tagtcagata gatgaggaga gttaaagatg
     9121 aacatattac tactctcata agaagtgccg gaatttcatt gtccagagta tcatttaaaa
     9181 aataatcttt cagaatatta actattaaaa aagtcagacc tccgctaaga aacattagta
     9241 aaaatttttt tagatgttcc gtaaatctgc caatgatctt tcttgaaaaa tacaaaagca
     9301 ttaatccata aattcctgta gtaataaaag tagccatcga gatcgcatag aatccaaaat
     9361 aaccgctgag taaataattc agcaaaattc ctgagagata tgtaatacta ccgatctttg
     9421 tcatgagcct gtattccttt tttgccagaa aaagtgctgt cgggacaatg tacattacaa
     9481 atgtcagcag cgaaagtgca tcccagaaaa atggtttcag tgtcatgtca attccgcttt
     9541 gatcaaaatt tccacgcaga taaaccattg acaatatatc ctttccaaat gtcaggaata
     9601 caactgtaac agggatcaac ataaacagca aaacggaaaa ggtattcaga aataaatttt
     9661 ttgctttgat ctttttttca aatgcaaata aagttgaaag gtccgagatc aagactgtaa
     9721 aaaatactga gaatatcaat gagaatatta cactcgtaag cgtacctgaa tagtgcagcg
     9781 atgaaactgc cccttcccca aaaaaagaag caaagtaatt tcgcgaaaga atatatatct
     9841 gattgcagat tacaagtaat aaaattgaag ctgatccctt tagtactttt tttgttactt
     9901 catctaattt taaaaaatca aatttgcgta atctgaactc tgcaccaagt ttcaccagtc
     9961 cgtaagtcat aactcctgcc attaaaaaat ttccggccag atttgcgaaa gacaaattat
    10021 acacaacatc atctttataa aaaggcatta ctattgatac taataaagct atgcctgtca
    10081 gcaccggtac gatcacaggc agaaaaaatt tcttaagagc attatataca gaatggaagt
    10141 aaccggtaaa tattttcagg aaaagtatgg ggaatattat atatgatatt tctatcgatc
    10201 tcaataattt ttctccttca aaaccaggca gaaggatttt gatcactgta ctgttgaaaa
    10261 taattataat agaaactaca actataccga tcagaaaaag tacgttgaat aaattattaa
    10321 atgatttcca tatcaggttt ttttcctggt ctttctgaag cgaagcgtat tcactgttta
    10381 cgacaccttt aattgtatca agcccgatca gcacaataag actgtcaggt atcattatca
    10441 aagcagtata aatatctgca gatgaagaaa ctccaagcac cgcagctatc acaagatttt
    10501 taaatacgtc aaatatcttt atcagaatta caataataaa cagcctggaa aatacacgtg
    10561 aagtactgtt cataattttt gtgattaatt ggaactgata attgcggact gtgaactttg
    10621 agacagtctg aatgtaaggg aaatcaggat ccagattaca atagtgatca caccgaataa
    10681 actcatatac ggttcgaaat taaatgcaac tataagccct acaaaagaga gctgcagacc
    10741 gtcaaaatat tttgaatata caggatcact tttttttcca ccaaatttga aagaataaac
    10801 agcagcttta aaaaagagat ataaataaat tacagcaccg atgagaccca tttcctgaat
    10861 agcgcgaaga taagtattat gaggatgggg cacttccact atataatatg ccatcacatc
    10921 gtatttatgc agataataag tatcaaaccc gtaacctaac aggggctttt cgattatcat
    10981 aaggatcgta gaataccatg cctgcagcct gccgagtgaa gaattgtcct gttcaaaatt
    11041 gatgacagat aaaactcttt cttctacaat attgtaaaat aaaagaataa tgatcccgat
    11101 aagtaacaga gaagggacaa tgatctttgg ctttctaata aaaaaaactg caatggaaaa
    11161 aataatggca agccatacac ctcttgtctt agtcagataa attccaagta aaataaatgt
    11221 aatagtaaaa aggtagaata cttttttctt tctgttgtca ttctttaata agaaagaaag
    11281 gttgaacata aatagtatca tcatatatgc agatacaata ttaaagtcat tccagaaaga
    11341 cggtacacgc atgtcactcc gcatattaaa aataatataa gtataaacag aatacattcc
    11401 gataactgta ccagagatca gcattgcatc aaaaaatttt gtcagtactt tttcatttaa
    11461 cctcagatta ttaaaaagga tgtaaataaa aaatggcagc agcattgtac cggtataata
    11521 tttcacagct tttaccggat ctggagtaat gtaacataca tatattcccc acagcagcat
    11581 cagagaccat gggatcatat tggagaattt tatttcatga tctttaaatg taaaatagcg
    11641 cagcatcata aatgcaaaag cggaaagtat aaataatgct gacaagtacc aattgccaac
    11701 aataaaagtg atcggtgtga gaataagtat cagctcagga gtgagcttaa tatcttcctg
    11761 acgaataatt tcaagtgagt gtgccttcaa ttctctttag ctgttttttt ataaaaaaga
    11821 atccgtatga aaaaataaat ccgagcacca tcactacaaa tatcgaagta agtctttttg
    11881 gtttggattt ttcatcaggt acaaatggct ggtcaagtac tattatagta ggtatctctt
    11941 ttttttcttc aatcttcgcc tgctcataaa gaggaagtaa taccgaaagt attttatttt
    12001 gaatctcaac atttcttttt agtctgataa aattcagtac tatatcaggt gttcctttta
    12061 actgcagttt tgaacttccg tcggtcgaat tctgcaggtc aaacaatctt ctttctaata
    12121 cattaatttt atcacgctga tactgaactt catcctcacc aggacttatg atcttctgaa
    12181 gtagttctaa tttaattttt tcagattcaa tttcagcttc gagttttatt tccagttcag
    12241 aaatttcctt tacctgaatc tcaggagaca acccataaat atcctgaaac tgttttagac
    12301 tgtcttccac aatgatcagt tctgcagagg atgcttcata tctttcctga ataaagtcac
    12361 gctgattttt tgcattctga cgattcattg caatattaat tttatcaagc tgagcgatca
    12421 gaaattcgga catctcctta gactttacag gatccttgtc atagactcct atatccattg
    12481 tacctgagac tttatctttc aaaatataca gaatattttc tcttacatct ttaattgcct
    12541 tctgcatatt cttgtaatca ttttcttcca tcaatccaaa tctgacaatg tactcttcca
    12601 gacatcttct gctgaggagt atatcttcat ataaagccag ctctttggag gaagaagaac
    12661 cggacagcac ttcaccgaac tcactgatct ccggtactga aattcccata cccaatccta
    12721 aaactttacc ggttgtctta acagtgccgt atgaaagaaa tatcggatct ataataaaat
    12781 aaacaagtac tgcagtcagt attccgagta tcagacaatt tgcaaaaatt ctttttttct
    12841 tttctttgta tatgccgata aagtctttta aagtaaattc catcttcgga gcccggacta
    12901 cttcttcagt gttatctgac attagataaa atttttgatg agataaattt gtaagcgata
    12961 aataaagaaa tgaatatgat tttcat
//
LOCUS       DAORVQ010000092        12874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2304_length_12874_cov_5.608316, whole genome shotgun sequence.
ACCESSION   DAORVQ010000092 DAORVQ010000000
VERSION     DAORVQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_12874_cov_5.608316"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLX80_08645"
     CDS             complement(<1..417)
                     /locus_tag="PLX80_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HQY20894.1"
                     /translation="MSEGKFNYEKESDKVEIIDANKLAVDRTNLAYERTALANDRTLM
                     AWIRTATSLTSFGFTIYKFFSDVVKVDTNLAVKRLITPRDVGLILIFFGILGLFFGLL
                     QYRKDMVRIRASYKAVELKSFTPVLAMLMIIFSLLLF"
     gene            560..1924
                     /locus_tag="PLX80_08650"
     CDS             560..1924
                     /locus_tag="PLX80_08650"
                     /inference="COORDINATES: protein motif:HMM:NF039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HQY20895.1"
                     /translation="MKIFTPVYILLFLIPFIFTENIFPQNFQKNPLSDTLKTITDLDK
                     TITDSVKSNTDSAKVTVVKIDSLKKKTLTKKNTKSDTLFNKDDHPQDITKGDGYYIYS
                     KNGKSVLRIYGSIRAFGSYDFDGLSGGTSFAISNIPVGPGRNDQSTFFMTANITRFGF
                     ESRRKFGFGNVFMKIETDFNGNNSTQFRIRHAFGQAVFLLAGQTWTVFSNVESIPNTV
                     DLDGPPTAVTLRTVQLRFFKDFSKSLHFKASIEAPKVSFSAPDSVTIEQVNQNFPDVA
                     LNLKKDFKNFEVSAASVFKSIKVIEISGSVGALKGGGVFIGGKSLITKGLTLYFQALY
                     GKGISSYLNISNNTATDVVLNVQTGNYVLTTSYGGFAALSQKLFKGHLDLNVIYGQIN
                     YDYEDYYPGSTFKSGQYFAFNAFYLTRAEFKLGIEYTYGFKRSMNNESGNANRTAFTF
                     YYDF"
     gene            complement(2030..4513)
                     /locus_tag="PLX80_08655"
     CDS             complement(2030..4513)
                     /locus_tag="PLX80_08655"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20896.1"
                     /translation="MKKFIIVTLLLLFPVISFSQDLQIPNNPYDAADENTKDRNAFKR
                     ERWFYEQRMFPDNFIPENSYAEAIDQRNELRQSQGFFLGANNTWTSIGPTSGFYFSYG
                     NISSRITTIKYDPVNPSIIYLGAAYGGVWKSVNGGDSWTSMTMDEASLSSGALAVDPV
                     NNNIVYYGTGEATYSGTSYYGRGLLKSTDGGTTWTNYTSGLQNFTFFSRIVIRPGHSN
                     ELLAALGSRSSLGATGGIYRSTDAGVTWSVIASGRCDDIVFSPDGQKAYAIGSGTSYR
                     ISTDGGSTFNTSTALSPLTRNHIAICKTSPNVLYSATHSGSSINVFKSTDGGVTFSPA
                     ASGTNFSGSQAWYDFYMYVNPFDPDYAYVGSIDVWRTTNGGTSFSNITFGYSGGNVHV
                     DQQNMDFHPTDPDKMMCVNDGGVWRSTDRGTSWSNLNSTLNLTQFYRIASDPSNASHI
                     LGGTQDNGTQRTLGTMNWEGAFGGDGGEVCFHDVDNNYILGETQNNGVQRSANGGASF
                     TSATNGLTGSGSWVAPLKSHPDSAGIFYTARQAVFKTTTWGASWFPISGSTGGTVREM
                     AISKSNPEIMYVTSGSAVLKSSDRGYTFTNVGAAVPNRTITGIYIHPDSSDVVLLCES
                     GFGGGHIFKSTNGGTNWSDISGNLPDSPANDLLIYHPGMSSSVYLIATDVGVFMSNNY
                     GTSWSELSDGLPNTVAMHFDYNEAASKLRIATHGRGVYEITNLTGVSSISNVNPESFS
                     LDQNYPNPFNPTTNISYNILSKGVVTLKVYNMLGSEVNTLVDKVQDAGSYNIHFNGSS
                     LTSGVYYYKLSFASGSNSFVDTKKMLLIK"
     gene            complement(4727..5386)
                     /locus_tag="PLX80_08660"
     CDS             complement(4727..5386)
                     /locus_tag="PLX80_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20897.1"
                     /translation="MKKIISILIFILFAKILFTNNSEAQTAKINLYSSYYFDDGLEAE
                     VNNISNLLGTVHGNYQWGAGVEYIFKETYGVELIYFGSNTSAVVDFLHNQAVSSDRTI
                     EMNSSFIMVSGNKYLPVKNSIVNFYGSLMAGMSIFIDKDPLPDKESTYKNFAWGLRLG
                     SEFKVSKYVGLNLNTQLLSTVQPAGRFYVDPRFVNAITSETSLYQFSLGGGLCIYFVG
                     K"
     gene            5857..7140
                     /gene="tig"
                     /locus_tag="PLX80_08665"
     CDS             5857..7140
                     /gene="tig"
                     /locus_tag="PLX80_08665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQY20898.1"
                     /translation="MDIKLNDKENSRKEIEATLTYEELTPYFDKAIESYRKKATLPGF
                     RKGKAPLNMIKKQFGEGIEYNALEDIAGEVFFKYITDNKLDVISKGAITDLDYKPKEK
                     LDFKVEFDVMPEVKIEKFKGLSLKKKVNIIDKSLVEDEINYHKFRSATYEIDGVAADD
                     DYMVTVNLQNLDEDGTILVGQSQNDLKVFLGNPEIYPEFKEGMSGIKEGEVRIIDSKN
                     AEGGLKKVQITCTKVEKIIYPEMNEEFFKSVTGKEGLKDEKEFKEELKGELLKIYDGI
                     TERELKSEVVNEMIKVNDVPAPERYIDVILDSMVEDYKQQYKGKKIPADFIEEEFRKE
                     RRVDAILQSKWFLLRDKLIEDEKIEIDEESYKKLAETYAARFNMPVDKLVEAYKQNED
                     VKMRLLNDKVIDLIIENAEIEEIPELRTKEDTKAN"
     gene            7163..7840
                     /locus_tag="PLX80_08670"
     CDS             7163..7840
                     /locus_tag="PLX80_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQY20899.1"
                     /translation="MNYTDIIKKNETLLHNSLDRINSINSQLVPIVVEQTSRGERSYD
                     IYSRLLKERIIFLGTAIYDDIASLLVAQLLFLESEDPDKDIMIYINSPGGSVTAGLGI
                     YDTMQYIKPDVTTMCVGMAASMGQLLLTGGAEGKRKALPHSKILMHQPWTGGLQGQTT
                     DILIHARDMEKTRETLFKIIAKHSGQTETKVMEDAERDNIMSAEEAKKYGLIDDILFQ
                     REKTEKK"
     gene            7894..10551
                     /locus_tag="PLX80_08675"
     CDS             7894..10551
                     /locus_tag="PLX80_08675"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQY20900.1"
                     /translation="MDFKLVNRIFAAFVFLFATIVYIMTVQPTFSFWDCGEFIACAYT
                     LSVPHPPGAPFFTLVGKIFTMLPTAVDIGLRANYLSVFSSSFCVLFLYLISTKVIKNW
                     RGVPKSTFDVLLICGSSMIGALSYAFSDSFWFNALEFEVYGMGTFLIGLCIYILMIWW
                     ERADEPGSDKYLLLVAYIVGLSLGIHLLVAQCIFLAGLFFYFKRYEYTPKTLAVAFGL
                     SAMAFFIVYPGLVKKFPLLMSGSVAIGFLIIGLLVFGIYYARVNKNPILSLITLSLFL
                     IIIGYSTYVSILMRANVTDLPINENTPSNIETLISYLNREQYGQQPLVMPRRYSQEPQ
                     HQAIYQNYSTDMEFMWKYQINHMFNRYWFWNYIGRAGYNQNDGVDFSKFFAIPFFFGM
                     FGLFYQFRKDWKLGFIFLMMFLMMGIITALYQNQQEPQPRERDYFYVGAFMAFSLWIG
                     IGVAGLIEVISEKMNKKSLLAVCSVVLLFSFAAVPANMLRVNYEYQNRNGNFFPYDYA
                     YNLLQSLDKDAILITNGDNDTFPLWCIQAAYGVRTDVRIINLSLAQTDWYNLQLKNER
                     PYGALTVPMTLTDAQLKKLQPVQWDEKKSVTYEVPITAYPDSMRNTPGLATKIVTTIP
                     ATIRQQSGTQVVTALKANDLLLMDIIKANKWERPIYFSVTVSDENYVGLGSYLVTEGM
                     GQRLVPYNPANFDGQDINVEVMNECLFNSPEVPSKTPLYGFRFKGLNDKKLFFNEDQT
                     RMAQTYRTVFMKLAFFYSNDSSKFAEAEKVLDRMETVIPRDVISMDYRIEYDVAMLYS
                     KIGNQQKFKELSDNVMLRAEEEIKNNPSNVQSYYNPYRILLDIYEARAEYQKALDLLN
                     RLSAMSPNDPSIMQKIESIKQKMSNPSGN"
     gene            10600..12618
                     /locus_tag="PLX80_08680"
     CDS             10600..12618
                     /locus_tag="PLX80_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20901.1"
                     /translation="MAKKNVKQNTSSLTKEKSSGKNILYFFLAALAVSFAFICFDNKF
                     LQDDAFISFRFIDNFVNGNGLVFNIGEKVEGYTNLLWVLILSGLKVMGMNIETASQNL
                     SVLFGVLILFITYFLSDIYKLTENALPKKGRAADSGSNVSYFDLIPSIMLVFTASFIF
                     WSVSGMETTMFMTFSLAGVYYYIKGKDSSELNYRFPLFIMLATLTRPEGMYFFGLIII
                     HKVFFAFKENGAGGLKSLFSKNEILSYLIYIVPIIFYFVIRYSYYGYLFPNTYYAKTG
                     MSEVYLNAGLDYFLNFLTSYMLYGILLIAPLYLFKRKENHFILSLFYLLIFSFIAYTI
                     VAGGDVLKQNRFFLTILPFIYILFAKLMTDIYYKISAKNSQSIAFGVVTVITIVICFY
                     YYSSQKEKLESDIQSENGLVEKMKSTGEWFRTKQLELGRPMTLAATTIGAVSYTAGPN
                     VTVIDLLGLTDEEIAHNPKIIPEISAGEIGWKERNYNADYVMSRDPDYINFSTGIKPS
                     AYGERALYTTEEFIKYYYPYYFTDKNTNSTDVVYKRKGDDEVLNYKHEFPGNPNYKNS
                     YVNMFNQAMNSSKTKSRFQDALNEFRLSMETGPAGFGLPYQYMGDMYLQAGNKKEAEE
                     SYKKAIEIDDYNVLAHYQLYQIYSEKGDTLKAMESIQKVQKYDPEVLR"
BASE COUNT         4364 a         2117 c         2343 g         4050 t
ORIGIN      
        1 aaacaataat aatgaaaata taatcatcag cattgcaagt acaggagtaa atgattttaa
       61 ttcaactgcc ttatatgaag ctctgatccg aaccatatct ttgcggtatt gtaataaacc
      121 aaaaaataaa cctagtattc caaaaaaaat aagaatcaga cctacatctc ttggagttat
      181 aagccttttg actgccagat tagtatcaac ttttacaaca tcagaaaaga atttgtatat
      241 agtaaatcca aatgatgtga gagatgttgc agttctgatc caagccatta atgtcctgtc
      301 atttgcaagg gcagttcttt cataagctaa atttgttctg tcgacagcaa gcttatttgc
      361 atctataatt tcaactttat cactttcttt ttcgtaattg aattttcctt ctgacataaa
      421 ataaaagaaa cagcgtttaa tttttgatgg gtttaattta attatctgat ctcaaaatta
      481 atagtaaaaa gcattgaaat tatttaatca atttattttt aaatatgatt ttataatatt
      541 agagccaatt taaaaatcta tgaaaatttt tacacctgtt tatattctgc tttttttaat
      601 tccatttatt ttcacggaaa atatcttccc gcaaaacttt caaaagaatc cgttgtctga
      661 tactttgaaa acaattacag atttggataa aacaattact gactcagtta aatcaaatac
      721 cgattctgcg aaggtaacag ttgtaaaaat tgattcttta aaaaagaaaa cgttaaccaa
      781 aaagaataca aaaagtgata cgcttttcaa caaagatgat catcctcagg atattacaaa
      841 aggagatgga tattatatat attcgaaaaa cggaaaatcg gtattaagaa tttacggatc
      901 gatcagagca ttcggatcat atgattttga cggattatcg ggcggaacat cttttgcaat
      961 cagcaacata cctgtgggac cgggcagaaa tgatcagagt acatttttta tgacggcaaa
     1021 tattacaaga ttcggatttg aatccagaag gaaatttgga tttggaaatg tgttcatgaa
     1081 aatcgaaact gactttaacg gaaacaacag tacacaattc aggatccggc atgctttcgg
     1141 acaggcagta tttttactgg ctggacagac atggactgta ttcagtaatg tagaatcaat
     1201 tccaaataca gtagatcttg acggaccacc tactgctgta actctaagga cagtacagct
     1261 aagatttttc aaagatttct ctaaaagctt acatttcaaa gcaagcatag aagctccgaa
     1321 ggtttctttc agtgcacctg attcagtaac cattgaacag gtaaatcaaa atttccctga
     1381 tgttgctttg aatttaaaaa aggatttcaa aaattttgaa gtgagtgctg ccagtgtttt
     1441 taagtcaata aaagttattg agatttcagg aagtgtcggg gcactgaaag gaggtggtgt
     1501 gtttattggt ggaaaatcgc tcattacaaa aggacttaca ttatattttc aggctttata
     1561 tggaaaagga atttcgtcat atttgaacat ctcaaataat actgcaactg atgtagtatt
     1621 aaatgtccag accggaaatt atgttcttac tacatcatac ggcggttttg ctgcacttag
     1681 tcaaaaatta ttcaaagggc atcttgatct gaatgttatc tacggtcaga tcaattatga
     1741 ttatgaggat tattaccccg gcagtacttt taagtccgga caatattttg cattcaatgc
     1801 attctatctg acaagagctg aatttaaact cggaatagaa tatacttacg gttttaaaag
     1861 gtcaatgaat aatgaatcag gaaatgcaaa cagaactgcg tttacttttt attatgattt
     1921 ttaaaaatat ttttaaaaca aaaaacaggc attgcaaata atctgcagtg cctgttttaa
     1981 attgtgtaaa atacaatatt cgaattatcg aaaatcatat ttataaaatt tatttgatca
     2041 gcagcatttt ttttgtatca acaaaactat tacttcccga tgcgaatgaa agtttataat
     2101 aataaacacc gcttgttaat gaacttccgt taaaatgaat attgtaactt cctgcatcct
     2161 gcactttatc taccaatgta ttcacttcac tgccaagcat gttatagact tttaaagtta
     2221 caactccttt gctcaaaata ttatagctga tatttgtagt tgggttaaaa gggttagggt
     2281 aattttgatc aagtgagaat gattcaggat ttacattgct gatacttgag acaccggtaa
     2341 gattagtaat ttcataaact cctcttccgt gagttgcgat ccttaatttt gaagcagctt
     2401 cattataatc aaaatgcatt gcaactgtat tgggtaatcc atcagaaagt tctgaccatg
     2461 aagttccata attatttgac ataaaaacac ctacgtcagt agcaattaaa taaacacttg
     2521 atgacattcc cggatgatat atcaaaagat catttgcagg ggagtccgga agattaccgc
     2581 tgatatcact ccagtttgtt ccgccgtttg tggatttaaa aatatgacct ccgccaaaac
     2641 ctgattcaca caatagtaca acatcagaag aatcgggatg aatataaatt cctgtaattg
     2701 tcctgtttgg aacagcagca ccaacgttag taaaagtata tcctctgtct gatgatttta
     2761 atacagcact tcctgatgtt acatacataa tctcaggatt cgatttgctg attgccattt
     2821 ctcttacagt tccgcctgta cttcccgaga ttggaaacca gcttgcaccc caggttgtgg
     2881 ttttgaaaac tgcctgcctt gcagtataaa aaatccctgc agaatcagga tgagatttca
     2941 acggagcaac ccaagatcct gaacccgtca aaccattggt agcagaagta aaagaagcac
     3001 ctccgtttgc agatctctga actccattat tttgtgtctc accaagaata taattattat
     3061 caacatcatg aaaacaaact tctccaccgt ctccgccaaa agcaccttcc cagttcatcg
     3121 taccgagagt tctttgagtc ccgttatcct gagtacctcc taagatatga gaagcattag
     3181 aaggatcaga agctattctg taaaactgag ttaagtttaa agtggaatta agattactcc
     3241 agctagtacc tctgtcagtg gatctccaga caccgccgtc atttacgcac atcatcttat
     3301 caggatcggt cggatgaaag tccatattct gctgatcgac atgaacattc cctcctgaat
     3361 atccaaaagt aatatttgaa aaacttgtac cgccgttagt agtcctccaa acatcaattg
     3421 aaccgacata agcataatct ggatcaaacg gatttacata catataaaaa tcataccatg
     3481 cctgtgatcc gctgaaatta gtaccggaag ctgccggaga aaaagtaact ccaccgtcgg
     3541 tagatttaaa cacattaata gaagatccgg aatgcgtagc agaatataaa acattgggag
     3601 aggttttaca gattgcaata tgatttcttg taagaggtga taaagctgta cttgtattaa
     3661 atgtcgatcc gccgtcagtt gaaattctgt atgaagttcc actgccgatt gcataagctt
     3721 tttgcccgtc aggtgagaat acaatatcat cgcatcttcc tgaagcaatt acgctccatg
     3781 tgacaccggc atctgtactt ctgtaaattc cgccggtcgc accaagagat gatctgcttc
     3841 ctaatgcagc cagcaattca ttgctgtgtc cgggtcttat tacaatcctt gaaaagaaag
     3901 taaaattctg aagaccggat gtatagtttg tccaggtcgt accgccgtct gtagatttga
     3961 gtaaacctct gccgtaatat gaagtaccgc tgtatgtcgc ttcaccggtt ccgtaataaa
     4021 ctatattgtt atttacagga tctacagcta atgcacctga acttaaagat gcttcatcca
     4081 tagtcatcga ggtccaggaa tctcctccgt taacagattt ccacacgccg ccgtaagctg
     4141 ctccaagata aataatggat gggtttaccg ggtcatattt aattgttgtt atccttgagg
     4201 aaatatttcc atatgaaaaa taaaaacctg atgtaggtcc aatgcttgtc catgtgttat
     4261 ttgcacccag aaaaaatccc tgcgattgtc ttaattcatt tctctggtca atagcttcag
     4321 cataagaatt ttccggaata aaattatccg gaaacatacg ctgctcataa aaccatcttt
     4381 ctcttttaaa agcatttcga tcttttgtat tttcatccgc cgcatcataa ggattatttg
     4441 ggatctgaag atcttgtgaa aatgatataa ccgggaataa taataatagt gttacaataa
     4501 taaatttttt cataataatt tttttttaaa atagtaataa taattaagat aaaaaagatt
     4561 ttcaaaaaag cttttctgaa aacgatttgc tcaaagacga ttaacctcaa agactcaaag
     4621 actcaaagtt aaaaacttta agaataagtt tcattgagcc ttcaatattt gagcaaactt
     4681 attaattttg tttcagggga gtatggatat gtgaaggaat taaaatctat tttccgacaa
     4741 aataaataca caaacctccg ccaagactga attgatataa agaagtttcg cttgtaattg
     4801 catttacaaa tctgggatcc acataaaatc ttcctgccgg ctggacggtt gataacaatt
     4861 gagtatttaa attcaaaccg acatacttag aaactttgaa ttcactgcca agtcttaatc
     4921 cccatgcaaa gtttttataa gtagattctt tgtcgggcaa tggatctttg tctatgaata
     4981 ttgacattcc ggccatcaga cttccataaa aattaactat ggaatttttt acaggtaaat
     5041 atttatttcc ggataccatt ataaaactgc tgttcatttc aattgttcta tcagaagaaa
     5101 cagcttgatt gtgaagaaaa tctacaacgg cacttgtatt agagccgaaa taaataagtt
     5161 caactccata agtttctttg aaaatatatt ccacacctgc tccccattga taatttccat
     5221 gaactgtccc tagcagatta ctgatattat taacttcggc ttctaatcca tcatcaaaat
     5281 aataagaaga gtataaattt attttagcag tttgagcttc tgaattattt gtaaatagaa
     5341 ttttagcgaa aagaataaat atcaggattg agatgatttt tttcatattt gttttaaatt
     5401 attggtttaa attttgtgat atgggtaatc tcatttttaa ttttgataaa ggtattgaat
     5461 ttaatttgaa atttaaatta taattataat ttaaatgctg aatctactaa gtttaattag
     5521 gtatcagaaa tttagtttag tatctacaga gtccgaagag cacatgactt tgcgggaaca
     5581 aaatctaagc taaattaaat tttgttaatt aatttacata gaataaccgg caatttatca
     5641 ccgcgggcga ttttttttat tacttttgag cctgcccgag tgtttttatc gggggtcgtt
     5701 aaaaaaaagt aagtagaaaa taatgaatct tgaattttgc ggctaaacgt tttgggcgaa
     5761 tgtgttaatt cacagttttc tcattattta attccttgtt acttcctgcc tatttgagta
     5821 attttactgc ttttaaaata aaaagtataa aataagttgg atattaagtt aaacgacaag
     5881 gaaaactcac gtaaagaaat tgaggcaacg ctcacatatg aagaactcac cccttacttt
     5941 gataaagcta tagaaagtta tagaaagaaa gcaactttac ccggattcag aaagggtaaa
     6001 gctcctttga atatgatcaa aaagcaattt ggtgaaggca tagaatataa tgcactagaa
     6061 gatattgcag gggaagtctt ttttaaatat ataaccgata ataaacttga cgtgatcagc
     6121 aaaggtgcca tcacagactt agattataaa cctaaggaaa aacttgactt caaagttgaa
     6181 tttgatgtca tgcctgaagt aaagatcgaa aaattcaaag gtctttcatt gaaaaagaaa
     6241 gttaatataa ttgataaatc acttgtcgag gatgagataa attatcataa attccgcagt
     6301 gcaacttatg aaattgacgg agttgctgca gatgatgatt atatggtaac agtcaatctg
     6361 cagaatttag atgaagacgg gacaatactg gtagggcagt cacagaatga tctgaaagta
     6421 tttcttggta atcctgaaat ttaccctgag tttaaagaag gtatgtcagg aataaaagaa
     6481 ggtgaagtca ggatcataga ttcaaaaaat gcagaaggcg gtctgaagaa agttcagata
     6541 acgtgtacaa aagtagagaa gatcatttat ccagaaatga atgaagaatt ttttaaatca
     6601 gtaacaggaa aagaaggact gaaagatgaa aaagaattta aagaagaact gaaaggtgag
     6661 cttctgaaaa tttatgacgg tatcactgaa agagaattga aatcagaagt agtaaatgaa
     6721 atgatcaagg tcaatgacgt acctgcacct gaaagatata ttgatgttat tcttgattcg
     6781 atggtagagg attataaaca gcagtataaa ggaaaaaaaa tacctgcgga tttcatcgaa
     6841 gaggagttca gaaaagaaag aagagtcgat gcgatattgc agtcgaagtg gtttctttta
     6901 agagataaac ttatcgaaga tgaaaagatt gaaatcgatg aggaaagtta taaaaaactt
     6961 gcagaaacct atgcagcaag atttaacatg cctgtagata aacttgtcga agcttataaa
     7021 caaaatgaag atgtaaaaat gagattgctt aatgacaaag taattgatct gataattgaa
     7081 aatgctgaga tcgaagagat acctgagctt agaaccaaag aagatacaaa agctaattaa
     7141 ttaaattaat aaataaaaat ttatgaacta cacagatata atcaaaaaaa acgaaacact
     7201 tctgcataac agccttgaca gaatcaatag tataaatagc cagcttgtac ctattgtggt
     7261 cgaacagact tcaagaggtg agagatctta tgatatatat tcgagactgt taaaggaaag
     7321 gataattttt cttggaacag caatttatga tgacattgct tcgcttttag tagcgcagtt
     7381 attattcctg gagtctgaag atccggataa agatattatg atctatataa attcaccggg
     7441 cggaagcgta acagcgggac ttggaattta tgatacgatg cagtatatca aacctgatgt
     7501 aactacaatg tgcgttggaa tggctgcttc aatgggacag cttttactta ccggtggagc
     7561 tgaaggaaaa aggaaagcac ttcctcattc aaaaattttg atgcatcagc cttggacagg
     7621 cggtctgcag ggtcagacta ctgatattct gattcatgca agagacatgg aaaaaacaag
     7681 agagacactt tttaaaataa ttgcaaagca ttcaggtcag actgaaacaa aagtaatgga
     7741 agacgctgaa agagataata taatgtcagc ggaggaagca aaaaaatatg gactcattga
     7801 tgatatttta tttcaaagag aaaaaaccga aaagaaatag cttacaataa aaagatcaac
     7861 agaatatata cttacaaata attaaatcat aagatggatt ttaaattagt taacaggatt
     7921 tttgcagcat ttgtattttt atttgctaca attgtctaca taatgactgt tcagccgact
     7981 ttttcattct gggattgcgg tgaatttata gcatgtgcat acacattatc agtaccacat
     8041 ccaccgggtg cccctttctt tactctggtt ggaaagatct ttacgatgtt accgactgca
     8101 gtagatatag gactcagggc aaattatctt tcagtattct cgagttcatt ttgtgtttta
     8161 ttcctttatc tgatctcaac taaagttata aaaaactgga gaggagttcc taaatcaact
     8221 tttgatgtac tgctgatttg cggatcttca atgattggag cactttctta tgcattcagt
     8281 gattcattct ggtttaatgc acttgagttt gaagtttacg gaatgggtac attcctgatc
     8341 ggtctttgta tatatatttt aatgatatgg tgggaaagag ctgatgagcc gggcagtgac
     8401 aaatatcttt tattagtcgc atacattgta ggtctttctc tcggaattca cttacttgta
     8461 gctcaatgta ttttccttgc aggacttttc ttttatttta aaagatatga atatacacca
     8521 aaaactttag cagtagcttt tggactttct gcaatggcat tctttattgt ctatccgggt
     8581 cttgtcaaaa aattcccgct gcttatgtca ggaagtgtgg caattggttt tcttataata
     8641 ggactactgg tctttggaat ttattatgcg agagtaaata aaaatccaat acttagtcta
     8701 atcacgttat ctttattttt aataattatc ggatattcta cttatgtctc tatattaatg
     8761 agagcaaatg taactgatct tccgataaat gaaaacacac cttcaaatat tgaaacactt
     8821 atttcatatc ttaacagaga gcagtatggt caacagccgc ttgtaatgcc gagaaggtat
     8881 tctcaggagc cgcagcatca ggcgatctat cagaattatt ctactgatat ggaatttatg
     8941 tggaagtatc agatcaatca tatgttcaat cgttactggt tttggaatta tatcggaagg
     9001 gcagggtata accaaaatga cggggttgac ttctcgaaat tttttgcaat accattcttc
     9061 ttcgggatgt tcggattatt ttatcagttc cggaaagact ggaagctcgg ttttatattc
     9121 ctgatgatgt ttttgatgat gggaattata acagccctct atcagaatca gcaggagccg
     9181 cagccgcgag aaagggatta tttttatgtc ggtgcattca tggcgttttc attatggatt
     9241 ggaattggtg tggcagggct tatagaagtc atatcagaaa agatgaataa aaaatctttg
     9301 ctggctgtat gttctgtagt actgcttttc agctttgcag ctgtacctgc gaatatgcta
     9361 agagtaaatt atgaatatca aaacagaaac ggaaatttct ttccttatga ttatgcatat
     9421 aatcttttgc agagtctcga taaagatgca attctaatta caaatggtga taatgatact
     9481 ttcccattgt ggtgtattca ggctgcatac ggcgtcagga ctgatgttag aataattaat
     9541 ttatctctcg cacagacaga ctggtataat cttcagttaa aaaatgaaag accttatggt
     9601 gctttgacag tgccaatgac attaaccgat gctcagttga aaaagcttca gcccgttcaa
     9661 tgggatgaga aaaaatcggt cacttatgaa gttccgataa cggcttatcc tgacagtatg
     9721 agaaatactc ctggactcgc tactaagata gttactacaa ttcctgctac tatcaggcag
     9781 cagtcgggaa cgcaggtggt tactgctctc aaagcaaatg atcttcttct catggatatt
     9841 ataaaagcca ataaatggga aagacctatt tacttctcag ttactgtttc ggatgaaaat
     9901 tatgttggac ttggcagtta tcttgtgaca gaaggaatgg gtcagagact tgttccatac
     9961 aatccggcaa atttcgatgg tcaggatatt aatgtagaag taatgaatga atgtttattc
    10021 aattctcctg aggttccttc caagactcca ttatatggat tcagatttaa gggattaaat
    10081 gataaaaagc ttttctttaa tgaagatcag acaagaatgg ctcagacata caggactgtt
    10141 ttcatgaaac ttgccttctt ctattcgaat gattccagta agtttgcaga agctgaaaaa
    10201 gttttggaca gaatggaaac tgtaattcct agagatgtaa tctcaatgga ttacagaatt
    10261 gaatatgacg tcgcgatgct ttacagtaag atcggtaatc agcaaaaatt caaagagctt
    10321 tcggacaatg tcatgctcag agcggaagaa gagattaaga ataacccatc aaacgttcag
    10381 tcttattata atccatacag aatactgctt gatatctatg aagcaagagc tgagtatcag
    10441 aaagctcttg acctgctcaa caggctgtct gcaatgagtc cgaatgatcc ttctattatg
    10501 cagaagatcg aatccattaa acaaaaaatg agtaatccat caggtaatta aataagttta
    10561 attcaatgaa cggtatttca aataccgttc attttttata tggcaaaaaa aaacgtcaaa
    10621 caaaatactt cttctcttac caaggaaaaa agttctggaa aaaatatcct ttattttttt
    10681 ctggctgctc ttgctgtcag ctttgcattt atttgttttg ataataaatt tttacaggac
    10741 gatgctttca tcagctttcg gtttatagat aattttgtaa atggaaacgg acttgtgttc
    10801 aatatcggtg agaaagttga aggatataca aacctgctct gggtgctgat ccttagcggt
    10861 ctgaaagtca tgggaatgaa cattgaaact gcctcgcaaa atctgagtgt tctcttcggc
    10921 gtgttaattc ttttcattac ttacttcctg tctgacattt ataagttaac tgaaaatgca
    10981 cttcccaaaa aaggaagagc tgcagattca ggatccaatg tcagttactt tgatctgata
    11041 ccttccatca tgcttgtatt tactgcatca tttattttct ggtcagtgag cgggatggaa
    11101 actacgatgt ttatgacgtt ctcacttgcc ggcgtttact attacatcaa aggtaaagac
    11161 agcagtgaat taaattacag atttccgtta ttcatcatgc tcgcaactct gacaagacct
    11221 gagggtatgt acttcttcgg actgataatt attcataaag tattttttgc atttaaagaa
    11281 aatggtgcag gtggtcttaa atctcttttc tcaaaaaatg aaattctatc ttatctgatt
    11341 tatatagttc cgataatctt ttattttgtg attagatatt cttattacgg atatttattc
    11401 ccaaatacct attatgcaaa gacaggtatg tcagaagtat atctgaatgc tggtcttgat
    11461 tattttctga atttcctgac gtcatatatg ctatatggaa tattgctgat tgcaccgctc
    11521 tatcttttta aaagaaaaga aaatcatttt atactttcac ttttttatct tctgatattt
    11581 tcattcattg catatactat agttgcaggc ggtgacgtac taaaacaaaa cagatttttt
    11641 ctgactatac ttccttttat ctatatactg tttgcaaaac tgatgacaga catttattat
    11701 aagatcagtg caaagaatag tcagagcatt gcattcggag tagttaccgt tatcacaatt
    11761 gtaatttgct tttattatta ttcttcacaa aaggaaaaac tcgaaagtga tattcaaagt
    11821 gaaaatggtt tagtggaaaa aatgaagagt accggtgaat ggttcagaac caaacagctt
    11881 gaactcggaa gaccaatgac acttgcagct actactattg gcgcagtatc ttatacagcc
    11941 ggtccgaatg ttactgtcat agatcttctc ggacttacag atgaagagat cgcgcataac
    12001 ccaaagatca ttcccgaaat ttcagcaggg gagatcggat ggaaggaaag aaattataat
    12061 gcggattatg tcatgtcgag agatcctgat tatattaatt tctcaaccgg tataaagccg
    12121 agcgcttacg gtgagagagc attatatact actgaggaat ttataaaata ttattacccg
    12181 tattatttta ctgataaaaa tacaaactct actgacgtcg tgtataaaag aaaaggtgat
    12241 gatgaagtat tgaattataa acatgaattt cccggtaatc cgaattacaa aaattcatat
    12301 gtgaacatgt tcaatcaggc gatgaactct tccaaaacca aatccagatt tcaggatgca
    12361 ttgaatgagt tcagactatc aatggaaacc ggtcccgcag gatttggtct tccttatcaa
    12421 tacatgggcg atatgtattt gcaagccggc aataaaaaag aagcggaaga aagttataaa
    12481 aaagctatag agatcgatga ttataatgta cttgctcatt atcagttgta tcagatatac
    12541 agtgaaaaag gtgatacgtt gaaggcaatg gaaagcattc agaaggttca gaaatatgat
    12601 cctgaagtac tgagataata tttaatttta aattttaaat attaaatatt aaaaattaat
    12661 ctgttaccac atagatcaat ttcaaaatga gtagaaaatt ttcactcaat ttttaaaaac
    12721 gaagtctgaa tttcaaattg tacgtttcag aataattcat tagtaattcc accttaaaaa
    12781 aggggagcaa aggggttgtt gcagaaagaa acagcaaaca gttattcagt aagctccaaa
    12841 ataaatttag ttagaacaac ttccgatgta tccg
//
LOCUS       DAORVQ010000093        12796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2333_length_12796_cov_5.239934, whole genome shotgun sequence.
ACCESSION   DAORVQ010000093 DAORVQ010000000
VERSION     DAORVQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_12796_cov_5.239934"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLX80_08685"
     CDS             <1..1021
                     /locus_tag="PLX80_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQY20902.1"
                     /translation="NVALRQKLDLYVCLRPVKYYGGIETPVRRPDKVDMVIFRENTED
                     IYAGIEFKAGTEDNTKFLKFLEENFPERFEKIRFGTLEKTNEFHKSGNIPLAEETEVG
                     IGIKVISKTGSHRLMKAAMNYAIENKRKSITIVHKGNIMKFTSGAFRDWCYELAEDQY
                     PEYVYTMMQWEKSFLAEGREAADKELNEAIDIGKIIIKDSIADNALQKVLTDPQDFDI
                     IASANLDGDYLSDAIAAEVGGIGIAPGANINFVTGHAIFEATHGTAPSFAGLDKVNPS
                     SLILSGMMMFKYLGWTEVSDFIEKGVRGAIKSKSVTFDFARQMVGAKEVKCSEFGQEI
                     INHFD"
     gene            1127..1714
                     /locus_tag="PLX80_08690"
     CDS             1127..1714
                     /locus_tag="PLX80_08690"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HQY20903.1"
                     /translation="MFKKLILTAFLSVGIFCIQNIASSQNDPATNLSKESNTEESKKS
                     ESDVKEEKSDQPEGIKMEDGILYGKNYEDGMEVVEFTDLMSNPADYKDKTLLVKGNIS
                     EVCQKMGCWLVLSDGSKNARVTTLHSFFVPKDATGSQVIVFGKFIEAELTEDQAKHYN
                     EESNKPMDETEIKGTSKVFEIEAEGVKILNISNEN"
     gene            1800..2348
                     /locus_tag="PLX80_08695"
     CDS             1800..2348
                     /locus_tag="PLX80_08695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY20904.1"
                     /translation="MEKDKLWKVISTEKGFIGDWINIDLDRIELPDKKEIVFEAIKFE
                     RNGAGIAAQNSDGKYILVKNYRYINDFESWEIPAGTIPPDMEPSECILEELKEEAGCI
                     ADKNDLKYHGYYYPSIGSSNQKFHCFTASNVKQITVEFDTNEIIETGWFSKHEILQMI
                     TDGIIKDGFSITLLMKSIFMTP"
     gene            complement(2335..2799)
                     /locus_tag="PLX80_08700"
     CDS             complement(2335..2799)
                     /locus_tag="PLX80_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20905.1"
                     /translation="MSKDPRNSLLVKQVEFVIVVRRTKILGIAITLGIVIIYLAGIFV
                     ADSYYIKDFETVNLLSLVQIFFLIPISFYIRKNILKSTNITNFTTKYFNAHIIPFMIV
                     DFSALFCITTNLFVNGNILYASIGAVIAAISMIMLFPKEEDFDEIKKDFKES"
     gene            2860..3906
                     /gene="ruvB"
                     /locus_tag="PLX80_08705"
     CDS             2860..3906
                     /gene="ruvB"
                     /locus_tag="PLX80_08705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQY20906.1"
                     /translation="MKSTDRINNFGSAGDEDTELHDYVSLIRPKRFRDFIGQEKVKSN
                     LKVFIDSSRKLNETLDHVLFTGPPGLGKTTLANIVSNELQTNIISTSGPVIERPGDLA
                     GMLTKLKKNEILFIDEIHRIPKVVEEFLYSAMEEFKLDIMIDQGPAARSIPIKLEKFT
                     LIGATTRQGLLSSPMLDRFGINCRLDYYQTENLIDIVKRSAKILDIKITEEGAVEIAK
                     RSRATPRIANRLLRRTRDFAVVKGNGSIDKEIADYALSSLDVDEFGLDKMDKKILETI
                     IFKFGGGPVGLSTIAASIGEDPGTIEDVYEPFLMIEGFIKRTPKGREATDLAYKHLKV
                     TRRKKMKDEKSLFE"
     gene            3944..4486
                     /locus_tag="PLX80_08710"
     CDS             3944..4486
                     /locus_tag="PLX80_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY20907.1"
                     /translation="MKSGNKIFKETLIILFVSLMVGLVANKISSKGIPLLRDDSDRFA
                     IDTNVIKQGEGKQPEKVLTKEGFVKPVNITVQVAKNLFDENAIFIDGREPHEFAEGHI
                     KGAINISYKEFIKKSPEERQKMMGDLDKEKTIVSYCGGGDCEISIDNAYEMAKIGYND
                     IKIYLGGYLEWNERGYPVNK"
     gene            4496..4984
                     /locus_tag="PLX80_08715"
     CDS             4496..4984
                     /locus_tag="PLX80_08715"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HQY20908.1"
                     /translation="MKILSNKYFLLVLRVIVGGVFIYASLDKLMNQEEFSRAIYNYKF
                     LPEVFVNIFAIVMPYLELFCGILLILGIFKGGSSFIITVMLLMFIIALSQAYFRGLDI
                     SCGCFSLETVSQKSDILQRIFEDILLILSSIIIFTQSLKPDITNNKNNINISNNKEKI
                     NE"
     gene            4977..5423
                     /locus_tag="PLX80_08720"
     CDS             4977..5423
                     /locus_tag="PLX80_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQY20909.1"
                     /translation="MSKLIQTKTAKTVYFVFGIFIISAVMFFLMNNSKISASGSTSKI
                     VFESEVHDFGTVPQGPQLDFSYKFTNKGKGSLVIEKVQPSCGCTGATTDGKNEYLKGE
                     SGEIKITFNTQGRQGRQEKQIIVFTNDPESPQKTLRLSCEIDPNIQ"
     gene            5501..7747
                     /locus_tag="PLX80_08725"
     CDS             5501..7747
                     /locus_tag="PLX80_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HQY20910.1"
                     /translation="MKYLITLFFTASLFLNAVGNTKNSIQNDHSELFKFNSAKTGNPD
                     AGVKDKVSDAIILNVDKDILNNIFSEKTYKLNLKIPVSGSDFVNAELERFDVLSDDAI
                     LISKGASGEIPFSSEGLILSYKGKVTGADESLISISIYDGKIIGLMKSKNETFVLGNL
                     TDRNNRDTEDYILYRESNLKNRHGVKCGSDIFGVPDEIISRIQNLNGQPIDAGTATLL
                     SSNIAVDVDFYTYGIYGNSVPNASAYALALMSAASAVYAKDMNIRLYVNYLRVWTVQD
                     PYTSDDGGTLLDQFRFDWINTQGSVTRTVAHLISRRNSINVGGIAYVNVLCNNSFGYG
                     LSSVQGNIGQLPNYSYDVVVVAHELGHNFGSPHTHSCSWVGGPIDSCYFTEGGCYNGP
                     LIPTQSTIMSYCDTEGGTVIMDFGTQPEALIRNSAENAAGCFGTAPQVYTAFPNGGET
                     FRTLTNTKIFWGTSVAGNVNIEFTTDNGGSWISIANNVPVQQREYDWTVPYIGYTNQA
                     KVRILNSANLNEGDTSDAAFKIILTYNSMTSLNPPSLARIETNAQNTNLNRFDWTSAG
                     AHPSFRYKVKFRKLGAGGVDYIFDADNNGTDTVLNIRNGVLDSIALLMGTVGDSVRCS
                     WRAWAYNGYDSAQTSNSAILTLIRTTVGINQISSVLPEKFDLGNNYPNPFNPVTKIKF
                     DVASAQQVKIIIYNNLGKKISELVNNKLQPGVYEVNFNGADMSSGVYFYTMETSDFVM
                     TKKMLLIK"
     gene            8099..8956
                     /locus_tag="PLX80_08730"
     CDS             8099..8956
                     /locus_tag="PLX80_08730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20911.1"
                     /translation="MINKYKKKAAELFKNKVKLTLRDNGYTQNFLPYDKTKNEYVIKH
                     SKNEYPVCDMGLPIPPAELRIGYETDNDWYIKNSRKDISTMISILEKSDFKIEGNKKI
                     LDFGCSSGRMIRSLKPYAETCEIWGTDVSADHIYWNNKYLNPPFHFATTTFNPHLPFE
                     DRYFDLIYSGSVFTHIDNLIESWLLELKRILSPGGRLYITIHENHTLEMITGNEGFPL
                     NKYFSSQPLFEEAKNDYDFFVFNRAKSPQVFFDLEYFKKMINPVFEILSVNYESYSYQ
                     TGVLLKRKS"
     gene            9234..10295
                     /locus_tag="PLX80_08735"
     CDS             9234..10295
                     /locus_tag="PLX80_08735"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY20912.1"
                     /translation="MTLSNKNKRNIKSILIFTVIIALGSAVNAFMEHGLEISWIIYDV
                     LIGITFGLIIGITEIYFFERKFRRVNFTVLLLIRTVFYVFVAVLIIILIAASDFGEST
                     DKILHDAMHDRIIRGIMESEDFNEIILYTVVLSFIINFIRQINHLLGQRVLLNYAAGK
                     YHSPIEEDLIVMFLDLKSSTTIAEKLGLIKNHRFLDDFFYDLTDPILESKCRIYQYVG
                     DEIVIVWKVKDGVKNLNCVNLFWDIEDEIESKKDIYLEKYGVYPEFKAGLHCGKVITG
                     EIGYIKKDIVYHGDTMNTASRIQSECNTHGKKLLISEELLNKLKINSKYKVVEIGSIL
                     LRGKEEEIDIFSIETNEKS"
     gene            10437..12512
                     /locus_tag="PLX80_08740"
     CDS             10437..12512
                     /locus_tag="PLX80_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20913.1"
                     /translation="MIIKEIFILLIFFSLADSNIFSQDLAPFSDQNSFIGNFKVTDNN
                     PFDSDNSVITVLNDDGTHSRHSAPQSLFRYARCYFLILASEMQASGFPSNVNITSLGY
                     NYKYGTDLAAKGFLKVYFQNTSDITNNKSLNWNSAVQGMSLVHADTLQFQTPPGIFDI
                     HLDSSENFIYTGGGIYVAFEYSNPGGPLAVVSNVVWANVIQPNPILKSSQSDDSLRVI
                     ATGTSNLRPETRFGCSLSDIVRTGPVYSMGMTGISLGSDSNFIKTVINHVRSEEDTIL
                     VTTSVKRISDGFVKHLFIDTVQSENIESIIITNKYLLESETGSDSILVNAFCSNEEIT
                     GNNNSFYINKITPHSWNHYITSMPPAGGVGLSVGTGDYVARFHTPSEIKICAADLSFF
                     ALTGFGYSPYKVIFYAADGPGGLPGTLLHKSPLRISPAGSTGNIKRSTYILDSALTIG
                     EGYYYAGYSQTDARNLRVSYQTEDPIRTGEFFYKQPAGGGSWIEFGDISPYRFDIAPR
                     TFLSLSLNLFLEGFYNGDEMIPDTIKIIVRNQTIPYSSVDSSQAVIDSAGHGLFNFIN
                     VNLDSCYYFDVRHRNHIRTFSHSTCENFNSAISVYDFTTTIDKAFGNNMKFITGINTS
                     GSFAIYGGDVNQDGLVDLADVSMIDNDVINFETGYLSTDANGDSLVDLADLLMADNNG
                     FNFVTKITP"
     gene            12603..>12796
                     /locus_tag="PLX80_08745"
     CDS             12603..>12796
                     /locus_tag="PLX80_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20914.1"
                     /translation="MKKSGSLFLKPIYLFLYIFILSNSFVFAQTDNVKLNIKSESNYQ
                     NPFIQQTSGSGLTVFALLFP"
BASE COUNT         4522 a         1838 c         2338 g         4098 t
ORIGIN      
        1 taatgtagct ttacgtcaga aactcgatct atatgtttgt ctaagacctg ttaaatatta
       61 tggtggaatt gaaactcctg taagacgacc tgataaagtt gatatggtaa ttttcagaga
      121 aaatactgaa gatatttatg caggtataga gtttaaagca ggaacagagg ataatacaaa
      181 atttttgaaa ttccttgaag aaaattttcc tgaaagattt gaaaagatca gatttggtac
      241 attagaaaaa accaacgaat ttcataaatc aggaaatatt ccacttgccg aagaaactga
      301 agtcggtatt ggaatcaagg tcatttctaa aaccggttcg catagactca tgaaagctgc
      361 aatgaattat gcaattgaga ataaaagaaa gtcgattaca attgtacata aaggaaatat
      421 tatgaagttc acatctggtg ctttcaggga ctggtgttat gagctggcag aagatcagta
      481 tcctgaatat gtttatacaa tgatgcaatg ggaaaaatca tttttagccg aaggaaggga
      541 agctgcggat aaagagttaa atgaggcaat agatattggt aaaattatta taaaagattc
      601 tatcgcagat aatgctcttc agaaagtcct taccgacccg caggattttg acataattgc
      661 ttctgctaat cttgacggag attatttgtc ggatgctatc gctgcagaag taggcggtat
      721 cggcattgca ccaggcgcaa atattaattt tgtaacaggg catgcaatct ttgaagctac
      781 acatggaact gcgccgtctt ttgccggact ggacaaagta aatccttctt ctcttatttt
      841 atccggtatg atgatgttta aatatctcgg atggactgaa gtttcagact tcatagaaaa
      901 gggagtcaga ggtgcgatca aaagtaaatc tgttacattt gattttgcaa gacagatggt
      961 aggagcaaaa gaggtaaaat gttcagaatt tggacaagag atcattaatc attttgattg
     1021 atctttgtac cggaataaaa gaaaatattt ttaaaattga tttctcgatt taatttaaca
     1081 gctaatttat ttaattttat aaaaacttaa aaaaggaaaa tttatcatgt ttaaaaaatt
     1141 aatactcaca gcatttttat ctgtaggaat attttgtatt caaaatattg cttcatctca
     1201 gaatgaccca gcaactaatt tgtctaaaga aagcaataca gaagaaagta agaaatcaga
     1261 atcggatgtt aaggaagaga aatctgatca gcccgaagga attaaaatgg aagatggaat
     1321 tttgtacggt aaaaattatg aagacggaat ggaagtagtg gaatttacag atctgatgag
     1381 caatcctgct gattacaaag acaaaacgtt actggtaaaa ggaaatattt ccgaagtttg
     1441 tcaaaaaatg ggttgctggc ttgttttatc tgatggcagc aagaatgcaa gagtcactac
     1501 attacacagt ttttttgttc ctaaagatgc tacaggttct caggtaattg tgtttggaaa
     1561 attcattgaa gcggaattga ctgaagatca ggcaaagcat tataatgaag aatcaaataa
     1621 accaatggat gagactgaaa taaaaggtac ttctaaagtt tttgaaatag aagcagaagg
     1681 tgttaaaatt cttaatattt caaatgaaaa ttaacagagc ataagtaata ttcatatttt
     1741 ttaaaagccc gttcaagcgg gctttgttat tttatcaaaa aaaccgtttt aaatttttta
     1801 tggaaaagga taaactctgg aaagtgatct caactgaaaa aggattcata ggagactgga
     1861 taaatattga tctggacagg atcgaattac ctgataaaaa agaaatagtt tttgaggcaa
     1921 taaaatttga aagaaacggt gcaggtattg ctgctcaaaa ttctgacgga aaatatatac
     1981 tggtaaaaaa ttacagatat ataaatgatt ttgaaagctg ggagattccg gctggtacta
     2041 tacctccgga tatggaaccg tctgaatgta ttctcgaaga gttaaaagaa gaagccggat
     2101 gtatcgctga taaaaatgat ctgaaatatc atggatatta ttacccctca attggttcca
     2161 gtaatcagaa atttcattgt tttacagcta gtaatgtaaa acaaattaca gtagaatttg
     2221 atacaaatga aataattgaa acgggttggt tttcaaaaca tgagatactt caaatgataa
     2281 cggatggaat aataaaagac ggcttcagta ttactttgct gatgaaaagt atttttatga
     2341 ctccttaaag tcttttttaa tttcatcaaa atcttcctct tttggaaata acattatcat
     2401 agaaatagca gcgataacag caccaataga agcataaaga atattaccgt ttacaaataa
     2461 attggtagtt atacaaaaca aagctgagaa gtctacaatc ataaaaggaa taatatgtgc
     2521 gttaaaatat ttggtagtaa agtttgtaat atttgtactt tttaatatgt tctttcttat
     2581 ataaaaggaa ataggaatga ggaaaaagat ctgaactaat gacagcagat ttacagtttc
     2641 aaaatctttt atataataac tgtcagcaac aaaaattccg gctaagtaaa tgatcacaat
     2701 tcctagagtg atagcaattc ccagaatttt tgttcttctg actacaataa caaattcaac
     2761 ctgctttacc aacaaactat ttctcggatc tttgctcaaa ttaaaattta taatgtttat
     2821 aaaattgttt gtgttccaaa ttaacattat ttttacaaaa tgaaaagtac agacaggata
     2881 aacaatttcg gctcggcagg agatgaagat acagaactgc atgattatgt ctcacttatc
     2941 agacctaaaa gattcagaga ttttataggt caggaaaaag taaaaagtaa tctgaaagtg
     3001 ttcattgaca gctcaaggaa actaaacgaa acccttgatc acgttctgtt tacaggacca
     3061 cccggtttgg gaaagactac acttgccaat attgtttcaa atgaacttca gacaaatatt
     3121 atttccacat cgggtccggt catagaaaga cctggtgatc tggctggaat gctgacaaag
     3181 cttaagaaaa atgaaatact ttttatagat gaaattcaca ggatacccaa agtagtagaa
     3241 gaatttttgt attcggcaat ggaggaattc aagcttgaca taatgataga tcagggaccg
     3301 gcagccagga gcattcctat aaagctggaa aaatttacac taatcggagc cactaccaga
     3361 cagggtcttc tttcttcacc aatgttagac agattcggta taaattgtcg cctcgattat
     3421 tatcagacag aaaatttaat agacattgtc aaaagatcag ctaagatact tgatattaaa
     3481 attaccgaag aaggtgcagt ggaaattgca aaaagaagca gggcaacgcc tcggatcgct
     3541 aacagacttt tgcgaagaac acgggatttt gcagtagtta aaggtaatgg atctatcgat
     3601 aaagaaattg ctgattatgc attatcatca cttgacgttg atgaatttgg tctggacaaa
     3661 atggataaga aaatactgga gacaattatt tttaaatttg gcggaggacc tgtaggattg
     3721 tcaacaatag cagcttctat cggtgaagat ccgggaacaa tagaagatgt gtatgaacca
     3781 tttttaatga tagagggttt catcaaacgc actccaaaag gaagggaagc aacagacctc
     3841 gcttataaac atcttaaagt aacccgaaga aaaaagatga aagatgaaaa aagtttattt
     3901 gaataaatat tcaatttaaa tattttaaat aattttaaaa tttatgaaat caggcaataa
     3961 aatttttaaa gaaacattga taatattatt tgtcagtctt atggtcgggc ttgttgcaaa
     4021 taaaataagt tcgaaaggaa ttccgttatt gagagatgac agtgacagat ttgcgatcga
     4081 tacaaatgtt ataaaacagg gtgaagggaa acagcctgaa aaagttttga ccaaagaagg
     4141 ttttgtaaag ccagttaaca ttaccgttca agttgcaaaa aatctattcg atgaaaatgc
     4201 aatttttatt gacggcagag agccgcacga atttgccgaa ggacatatta aaggtgctat
     4261 aaatatttct tataaggaat ttataaaaaa atctcctgaa gagagacaga aaatgatggg
     4321 agatcttgac aaggaaaaaa caattgtttc atattgtggc ggcggagact gtgagatcag
     4381 cattgacaat gcatatgaaa tggcaaagat cggttataac gatatcaaga tatatctcgg
     4441 aggttatctt gagtggaatg aacgtggcta tcctgttaat aaataaacta aattaatgaa
     4501 aatactttca aataaatatt ttttactggt tctcagagtc atagtcggag gcgtatttat
     4561 ttatgcatct ttagataagc tgatgaatca ggaggagttt tcaagagcta tttacaatta
     4621 caaattcctg cctgaagttt ttgtaaacat ttttgcaatt gtaatgccat atctggaatt
     4681 attctgcggc atattgctga tattaggcat ttttaaagga ggcagttctt ttataataac
     4741 tgttatgctt ttaatgttta ttatagcatt atctcaggca tattttcggg gacttgatat
     4801 aagctgcgga tgtttctcac ttgaaactgt aagtcagaaa agtgatattt tgcaaagaat
     4861 ttttgaagat atattgctta ttttatcttc aattattatt tttacacaga gtttaaaacc
     4921 agatataact aataacaaaa ataatattaa catttctaat aataaggaga aaataaatga
     4981 gtaagttaat tcaaacaaaa acagcaaaaa cagtttattt cgtttttgga atttttatta
     5041 tttctgcagt aatgttcttt ttaatgaaca attcaaagat ctctgcttca ggaagtactt
     5101 caaaaatagt atttgaaagc gaagttcatg actttggaac agtaccgcaa ggacctcagt
     5161 tagacttttc gtataagttt actaataaag gaaaaggaag tcttgtaatt gaaaaagttc
     5221 agccatcctg cggatgtaca ggtgctacta ctgacggaaa gaatgaatat ttaaaaggag
     5281 aaagcggaga aataaaaatc acatttaaca cacagggaag acagggaaga caggaaaaac
     5341 aaattattgt ttttacaaat gatcctgaat ctcctcaaaa gactttaaga ctttcatgtg
     5401 aaatagatcc aaacattcag taatctcaaa cgaataattt ttatatgcag ggattgtttt
     5461 acagttcctg catttttttt attaactata tataattaaa atgaaatatt taattaccct
     5521 tttctttact gcctcacttt ttttaaatgc tgtaggcaat acaaaaaatt caattcaaaa
     5581 tgatcattca gaacttttta aatttaattc tgcaaaaacc ggtaatccgg atgcaggtgt
     5641 taaagacaaa gtaagtgacg caataattct taatgtagat aaagatattt taaataatat
     5701 tttttcggaa aaaacttaca agcttaattt aaagatccct gtttcaggat cagattttgt
     5761 aaatgcagag ctggaaagat ttgatgtact ttccgatgac gcaatcctta taagtaaagg
     5821 tgcttcaggt gaaattcctt tttcttcaga aggtctgata ctttcttata aagggaaagt
     5881 tacaggtgct gatgaatcac tgatctcaat ttctatttat gacggaaaaa ttataggact
     5941 catgaaatcg aaaaatgaga cttttgtact tggtaatctg actgacagaa ataacagaga
     6001 tactgaagat tatatattgt atcgagaaag taatttaaaa aaccgtcacg gtgtcaaatg
     6061 cggatctgac atttttggag tgcctgacga aataatcagc agaattcaaa atctgaacgg
     6121 acaacctatt gacgccggta ctgcgacttt actttcatcg aatattgcag tcgatgtaga
     6181 tttttatact tacggcattt acggaaattc tgtaccaaat gcatctgcct atgcacttgc
     6241 attgatgtcg gcagcttcag cagtttatgc caaagatatg aatatcagac tttatgttaa
     6301 ttatttaaga gtctggactg tgcaggatcc atatacttct gatgacggag gtactttgct
     6361 ggatcagttt agatttgact ggataaatac acagggcagt gtaacaagaa ctgtagctca
     6421 tcttatcagc agaagaaaca gcattaatgt cggcgggatt gcttatgtga atgtactttg
     6481 taataattct ttcggatacg gactgagttc agtgcaggga aatatcggac agcttccaaa
     6541 ttattcttat gacgttgtag tagttgctca tgaacttgga cataatttcg gttcaccaca
     6601 cacacattcc tgttcatggg tcggcggtcc gattgattcc tgttatttta cagaaggagg
     6661 atgttacaat ggtccgctta tcccgactca aagtacaata atgagttatt gtgatacaga
     6721 aggcggtact gttataatgg attttggaac acagcctgaa gcactcataa gaaatagtgc
     6781 agaaaatgct gcaggatgtt ttggtacagc accacaggtc tatactgctt ttccaaatgg
     6841 aggagagact ttcaggacgc tgacaaatac aaaaatattc tgggggacat ctgttgccgg
     6901 aaatgttaac attgaattta caactgataa cggcggaagc tggatttcaa tagcaaataa
     6961 tgtcccagtt caacaaagag agtatgactg gacagttccg tacattggat atactaatca
     7021 ggcaaaggtg agaattttaa attctgcaaa tttaaatgaa ggtgatacaa gcgatgcagc
     7081 tttcaaaatt attcttactt ataattcaat gacgtctcta aatcctccgt cattagcgag
     7141 gatagaaacg aatgctcaga acacaaatct gaatagattt gactggacca gtgcaggtgc
     7201 acatccgtca ttcagatata aggtaaaatt cagaaagtta ggagccggag gagtcgatta
     7261 tatttttgat gcggacaata acggaactga tactgtatta aatatcagaa atggagttct
     7321 tgattctata gcattactga tggggacggt tggagattct gtcagatgtt catggagagc
     7381 atgggcttac aacggatatg attcagctca aacatccaac agtgcgatcc tgactttaat
     7441 aagaactacg gttggtataa atcagatatc ttcagtcctt cctgagaaat ttgatctggg
     7501 aaataattat ccaaatccat tcaatcctgt tacaaagatt aaatttgatg tagccagtgc
     7561 tcagcaggta aaaataatta tttataataa tcttggaaag aaaatatctg aacttgtaaa
     7621 taacaaatta cagcctggtg tgtatgaagt gaattttaac ggagcggata tgtccagcgg
     7681 agtttatttt tatacaatgg agacgagtga ttttgtgatg acaaagaaaa tgctgctgat
     7741 aaagtagaga gcaattaaaa atcagaaatt agaaattaag aattattcta aaacgcaatg
     7801 tccgggttaa tccggacatt gcgtttttta atttgtataa atttaaacag ggattaaacc
     7861 gtttcaggtg tttcaataaa gtttcaaagc aattcattta tatacttatg agtctcttat
     7921 actattagtt tagtaaaaaa gtagattgca gctcaggatc ttcaatgcaa aataatatta
     7981 tcatcggtgg cattttttat ttttaaaaaa cttatctgta aaaattagta tagaaaatac
     8041 atattggatt ttatatactg ctaaactaat attaaatcag tacatcattt aaaagatcat
     8101 gatcaataaa tataaaaaga aagctgctga actttttaaa aataaagtga agcttacttt
     8161 gagagataat ggatatacac aaaacttcct tccttatgat aaaacaaaaa atgaatatgt
     8221 tataaaacat tctaaaaacg aatatccggt gtgtgacatg ggacttccta ttcctccggc
     8281 tgagcttaga attggttatg aaactgacaa tgactggtac ataaaaaatt caagaaaaga
     8341 tatttccact atgatcagca tacttgagaa atctgatttt aaaatcgagg ggaataaaaa
     8401 aattcttgat tttggatgca gctccggaag aatgatacga agtctgaagc cgtatgcaga
     8461 gacctgcgag atatggggaa cagacgtgag tgcagatcat atttactgga acaataaata
     8521 tcttaatccg cctttccatt ttgctactac tacttttaat ccccatcttc catttgaaga
     8581 cagatatttt gatcttatat attcgggatc ggtattcact catatagata atctgatcga
     8641 gagctggctt cttgaactta aaagaattct ctctcccggg ggaagattat atattacaat
     8701 tcatgaaaat catacacttg aaatgatcac cggtaatgaa ggctttcctt taaataaata
     8761 tttcagttca cagccgttat ttgaagaagc taaaaatgat tacgactttt ttgtttttaa
     8821 cagagcaaaa tcgcctcagg tattttttga tttagagtat ttcaaaaaaa tgatcaatcc
     8881 tgtttttgaa attttatcag taaattatga gtcttacagt tatcagacag gcgtactgct
     8941 gaaaaggaaa agttaaaaaa ttcatttctt ttattatcgg aatacagaaa tatgaaataa
     9001 aatattacat atagaaataa ctttagtcta taaaattatt tccctcaaaa tctttaacat
     9061 ctttcgtatt taagaataca cattaatttc ttatcttgat gactcagatt aaattttttt
     9121 aacaaattag aattccgaaa aatataagtt atagaaattt ccattctaag tttttagatt
     9181 taaaaactta tttgatatta ttatgcatta attttagtaa aaaattttat tgaatgacac
     9241 tttctaataa gaataaaaga aacattaaat ccattctgat ctttacagtc ataattgctt
     9301 taggctcggc tgttaatgct tttatggagc atggacttga aatatcctgg attatttatg
     9361 atgttttaat tgggattaca tttggattaa taattggaat aacggagatc tatttttttg
     9421 aaagaaagtt caggagagta aattttactg ttctcttatt aatccgtaca gttttttatg
     9481 tcttcgtagc agtattaata ataattttaa tagcagcttc tgactttggt gaaagtactg
     9541 ataaaattct gcatgatgca atgcatgata gaattatccg cggaattatg gaaagtgaag
     9601 acttcaatga gatcatatta tacacagttg ttttaagttt tatcataaat tttatccggc
     9661 agataaatca tcttttaggt cagagagtgc ttctgaatta tgcagcaggg aaatatcatt
     9721 cacctataga ggaagatctt attgttatgt ttcttgatct gaaatcctcc actactatag
     9781 ctgagaaatt agggctgata aaaaaccaca gattcctaga tgattttttt tatgatctca
     9841 ctgatccgat tcttgaaagt aaatgcagaa tttatcagta tgtcggagat gaaatagtaa
     9901 ttgtctggaa agtaaaggat ggtgtaaaaa atctaaactg cgttaattta ttctgggaca
     9961 ttgaagatga gatagaaagt aaaaaagata tttatttaga aaaatatggt gtgtatcctg
    10021 aattcaaagc aggacttcat tgcggaaaag ttattaccgg tgagatcgga tacataaaaa
    10081 aagatatagt ttatcacgga gatacaatga atactgcttc gcgtattcag tctgaatgta
    10141 atacacacgg aaagaaatta ctgatatcgg aagagctttt aaataaacta aaaattaatt
    10201 cgaaatataa agtagtagaa ataggttcga tacttttaag aggtaaagag gaagagatag
    10261 atattttttc gattgaaaca aatgaaaaga gttaatagaa tttatataat cttattttga
    10321 ttattgaagt actgaatatt ttatatttta aaatttaatg tttcttaatt ataacgtttt
    10381 ctttttttaa aattcttatc agagaatcaa atagttttaa caaaataaaa ctaagtatga
    10441 tcataaaaga aatttttata ttattaatat ttttttcatt agcagattca aacatctttt
    10501 ctcaggatct ggcaccattc tcagatcaaa attcttttat tggtaatttt aaagtaacag
    10561 ataataatcc ctttgactca gataattcgg taattacagt tttaaatgat gatggtacac
    10621 attcaagaca tagtgctcct caatcattat tcagatatgc aagatgttat tttctgatac
    10681 ttgcctcaga aatgcaggca tcaggatttc cgtcaaatgt aaatattact tcactcggat
    10741 ataattataa atacggaact gaccttgctg ccaaaggttt tctaaaagtt tattttcaaa
    10801 acacttctga cattacaaac aataaatctc tcaactggaa ttctgcagtg cagggcatgt
    10861 ctttagttca tgcagataca cttcagtttc agacaccgcc cggaatattt gatattcatc
    10921 tggattcttc tgaaaacttc atttatacag gtggcgggat ctatgttgca ttcgaatatt
    10981 caaatccagg cggtccgctt gctgtagttt caaatgtggt atgggcaaat gttattcagc
    11041 caaatcctat tttaaaatct tcacaatcag atgacagtct cagagtgatt gctaccggca
    11101 cttctaatct acgtcctgag acaagattcg gatgtagtct tagtgacata gtcagaacag
    11161 gtcctgtata ttcaatggga atgacaggaa tttcattagg aagtgattct aattttatta
    11221 agactgtgat aaatcatgtg cgatctgaag aagatacaat tttagtaaca acatctgtta
    11281 aaagaatttc cgatggtttt gtaaaacatt tatttattga tactgtccaa agtgaaaaca
    11341 tagaaagtat tatcatcact aataaatatt tacttgaatc tgaaacagga agtgattcga
    11401 ttttggtaaa tgctttttgt tctaacgaag aaatcacagg aaataacaat tctttttata
    11461 ttaataagat caccccgcat tcatggaatc attatataac atcaatgcct ccggctggag
    11521 gagttggatt aagtgtaggt acaggggatt atgttgcaag atttcatact ccttctgaaa
    11581 taaaaatatg tgctgcagac ctgtcatttt ttgctctgac aggttttggt tatagtcctt
    11641 ataaagtaat attttatgca gctgacgggc cgggcggttt acccggaacg ctgttgcata
    11701 aatcaccgct gcggatttca cctgcaggat caacaggaaa tattaaaagg tcaacttata
    11761 ttttggattc tgctttgaca ataggtgaag gttattatta tgcaggatac tcacaaacag
    11821 atgcgcgaaa tttaagagta agctatcaga ccgaagatcc aattcgaaca ggagaatttt
    11881 tttataaaca acctgccgga ggaggcagct ggatagaatt tggagatata tcaccttaca
    11941 ggtttgatat agctccacgg acatttcttt ctctctcatt gaatttattt ttggaaggat
    12001 tttataatgg tgatgaaatg atccctgata ccataaaaat tattgtaaga aatcagacaa
    12061 ttccctacag ttctgttgat tcatctcagg cagttataga ttctgcaggt catggtctgt
    12121 ttaattttat aaatgtaaat ttagattcat gttactattt tgatgttcgt cacagaaatc
    12181 atatcagaac ctttagtcac agtacatgtg aaaattttaa ttcggcaatc tcagtatatg
    12241 attttacaac tactattgat aaggctttcg gaaataatat gaaatttatt actggtataa
    12301 atacatcagg ttcatttgct atttatggag gcgatgtaaa tcaagacggt cttgtagatc
    12361 ttgcggatgt aagtatgatc gataatgatg taataaattt tgagactggg tatctttcca
    12421 ctgatgcaaa tggtgatagt cttgtagatc ttgctgattt attgatggct gataacaatg
    12481 gatttaattt tgtgacgaaa atcacacctt gagctaatgt tcaatgttta attttaaatg
    12541 ttcaattttt aatgataaaa ctaaaatttt gtttgaattg agaaaatcat ttttaattcc
    12601 caatgaaaaa atctggttca ctttttctta aaccaattta tctgttttta tatattttta
    12661 ttctttcaaa ttctttcgtg tttgctcaga ctgataatgt aaaattaaat atcaaatcag
    12721 aatcaaatta ccaaaatcct tttatacagc agacttcagg aagtggactg acagtatttg
    12781 cattactgtt tccttt
//
LOCUS       DAORVQ010000094        12334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2466_length_12334_cov_5.594592, whole genome shotgun sequence.
ACCESSION   DAORVQ010000094 DAORVQ010000000
VERSION     DAORVQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2466_length_12334_cov_5.594592"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..900
                     /locus_tag="PLX80_08750"
     CDS             259..900
                     /locus_tag="PLX80_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQY20915.1"
                     /translation="MLIRRDGVTVTRADFLQLLDYAVPQEKRAAVLADENKIREMRDK
                     YYNISFQYSGKINWHMVGHLQSNKVKDIIAFIHLIHSVDTFNLAEVIDSTAKRINRKI
                     DILVQVNTSNEEQKYGVLPEEAESLCKQISLLENINVKGLMTIARLTDDRDEIRRSFR
                     TLRELFDKMKSDFSDFKYLSMGMTNDFDIAIEEGSNMLRIGSAIFGERPVIVE"
     gene            983..1570
                     /locus_tag="PLX80_08755"
     CDS             983..1570
                     /locus_tag="PLX80_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQY20916.1"
                     /translation="MSDKILPKEIKKTDFKKSFRGYESAEVDAFLETVSLRYERLYEE
                     NVSLLEQIKNLNSDLQIYKENESTLQKAIVKSQDLTEEIIDTAKKKSENIIKEAELNS
                     QKILQDSDKEIMTRKHELEELKLRNDKLVEDVKLFFMEKLNEMDEFVKSRNIYKMELS
                     KHDHAEDSEEELPKEEMPAIIKKISINTANSDFSE"
     gene            1583..2380
                     /locus_tag="PLX80_08760"
     CDS             1583..2380
                     /locus_tag="PLX80_08760"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQY20917.1"
                     /translation="MTDKHLEALSHLTNQFGNRPETGIILGTGLGGLVKYIDIDLEVE
                     YSDIPNFPVSTVESHSGKLIFGRLSGKNIVAMQGRFHFYEGYSMDEVTFPIYILKSLG
                     VKNLIVSNACGALNQKFNKADLMIMTSHINLLFDTPLKRKKLNYTDGSKIYSKRLIDM
                     AESTAIESGISIQKGVYAPVQGPNLETVSEYRMIRKMGGDTVGMSTIPEALIASNLGM
                     EVLGLSIITDKGFPDTLEVAVLKDILESASIAEPKLTLLIKNLAAKL"
     gene            2432..3274
                     /locus_tag="PLX80_08765"
     CDS             2432..3274
                     /locus_tag="PLX80_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQY20918.1"
                     /translation="MTEQSEKINQSAEYIKSRFPKKYKPELCIITDNFQEIAKEFKSS
                     GDLKYIDIPGFKTSNDKENSGKIIFAKSKKKDVLIFSGRPHYYDGTDMRSIGHRIYTL
                     KFLGIKKIISISETAHLNPRFSCGELAVIFDHINLMGDNPLIGKNDDELGLRFPDMSN
                     AYNKDLYKSLYSIFQNNKIRINESVLLGITGPESETEAEARFYREIGADVLGYSVVPE
                     NITAVHCRIEFAAIGLISRELIADKMLDDVTSEKQKIKDQSGHLGSAVKLLKGIISEI
                     ADKI"
     gene            3421..4296
                     /locus_tag="PLX80_08770"
     CDS             3421..4296
                     /locus_tag="PLX80_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY20919.1"
                     /translation="MQDRLEIPKMYKLFIGGKFTRTESERYFDALNLKDGKKICNISR
                     ASRKDIRNSVVAARSGFNDWSGKTAYERGQIMYRFAEMLEGRKEQFIDEIFISTKESK
                     SDCKKEVQKAVDRLIYFAGFCDKWTQLSGSVNPVQSGYFNFSIPEPTGIVGIILPDTL
                     SFLPLISRVSAVLSSGNSCVIIANESSPLPALSVAEVIATSDFPSGVINILTGLKSEL
                     NSHLAGHFDVNAIDLCTGIRSDKKTIQELCANNVKRFSSIPENHDWYSDKDNENLKEI
                     EKFTELKTVWHTMGM"
     gene            4439..4909
                     /locus_tag="PLX80_08775"
     CDS             4439..4909
                     /locus_tag="PLX80_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HQY20920.1"
                     /translation="MSNITKLIISIALCQMAGIIGTFFTMDSIPGWYEGLTKPSFNPP
                     NWIFGPVWILLYTLMGVSLFLVWKEGLQNADVKKAFYIFMIQLVLNSAWSIVFFGMES
                     TSGGLIVIFLLWIMILVTIYNFLKISKASGLLLLPYFLWVSFATLLNYFIFKLN"
     gene            4920..6053
                     /locus_tag="PLX80_08780"
     CDS             4920..6053
                     /locus_tag="PLX80_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY20921.1"
                     /translation="MILFRNTWDRFQSFLFILIAILISSNAFAEENLLKDSVNISGKI
                     NSEKMFFETTDISSSDDMIIYHMKEKFPIISESDLNKIISTKSVISIADPDTITGIQT
                     EEKFKVSFYVPENFLVDSTKIFWELNIPEFQSKIYQLYNGQNIYIDTWPNVVGTMKDK
                     TYTGNFEVYRVRNYPFYKDPDPKKAHLSPTPPGKGNPLGLFVVHYDENSLRYFHGNNN
                     NKLLYSKMRNLSHGCVRNDNDNIEEMKQFIIKKVIKSEDLSGWLDSKKTLVYNLEESD
                     KFPVKIIYKTYEIDKDNSGYYVTLFKDIYNYSSGRINETLNDPGLIFLSNNENLFAEY
                     RKVVGNDISDDALNILVEYVLNKGIEYEKLYISDLKNSLSISN"
     gene            complement(6050..6580)
                     /locus_tag="PLX80_08785"
     CDS             complement(6050..6580)
                     /locus_tag="PLX80_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20922.1"
                     /translation="MSHIKLNTAFKYFFPIAIIVTLISFASCENIFSPKLDLSTSTSI
                     ITDQKTVEGVFQNFKYAYTFEDTTVYGNLLGEQFLFIYRDYNSGFDISWDRATEMRTT
                     NGLFQNAQKLEVVWNNIIFEGGDSLVRNVKRSFNLTITFNPSDVVRLNGFADMNLVRP
                     STNDVWKIIRWRDETF"
     gene            complement(6584..7171)
                     /locus_tag="PLX80_08790"
     CDS             complement(6584..7171)
                     /locus_tag="PLX80_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20923.1"
                     /translation="MLKRPDKILILIFILYSGCGLFETRSVEPPTENRSTFTQPTSPD
                     IVLANLNFAVTEKNLDNYMRCLVDSNFSTKRFKFIPDAVSQTAYPVFLNWNLSSERIY
                     FSNLISSTEENSSTNLFLSNINFNTGIDSAVIDSDYILVFNHNRQNIAQVTKGRLRFV
                     MSPDVRSLWSIHSWSDFINDNGDTTWSVLKANMFN"
     gene            complement(7155..7895)
                     /locus_tag="PLX80_08795"
     CDS             complement(7155..7895)
                     /locus_tag="PLX80_08795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY20924.1"
                     /translation="MWYKEWFSDKLYLDIYSHRNEEEAGHMIDLIQRNIDIITGAKVL
                     DVACGAGRHSLQLAKRGFHVTGFDLSEFLISEAKKAHKEAKENDLKVKFLIRDMRKFN
                     FKNSFDLAVNIFTSFGYFDNDEENFSVIKNISDSLKKGGYFVFDFINSEFIKNNIVPL
                     SRNKFGEIKVIQKRRIENGFIIKDIEVKKDTEVRNFKEILKLYNRKELVKCFGEFNFK
                     VKTLFGDYYGNKFDEKKSKRLIIIAKKT"
     gene            complement(7992..8600)
                     /gene="recR"
                     /locus_tag="PLX80_08800"
     CDS             complement(7992..8600)
                     /gene="recR"
                     /locus_tag="PLX80_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQY20925.1"
                     /translation="MVNISNALENLVDEFARLPQIGRKTAIRLAMHITRQPREDVERF
                     SRALTEIKEKLRFCSVCCNITEDEICNICRSGSRKGNMICVVEEPQDVFAIEKTNEFK
                     GKYHVLHGIISPLDGIGPNDIRIKELLERLGSNVDDKVEELILALNPTVEGETTILYL
                     NKLLKPLELKITRIARGIPVGSDLEFADEVTLARALEGRILI"
     gene            complement(8770..9105)
                     /locus_tag="PLX80_08805"
     CDS             complement(8770..9105)
                     /locus_tag="PLX80_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQY20926.1"
                     /translation="MNQGQMQGMMKQIKKMQDKMDKIQEELEGKSVTEESGGGMVKVT
                     VNGKNRITKIEIEKEIINPEDPQMLEDLIIAAVNKGLESSQKMADEEMSKATSGMMPN
                     IPGMNLPKF"
     gene            complement(9174..9893)
                     /gene="bshB1"
                     /locus_tag="PLX80_08810"
     CDS             complement(9174..9893)
                     /gene="bshB1"
                     /locus_tag="PLX80_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HQY20927.1"
                     /translation="MNPEKKLDVLFFAAHPDDAELNCGGTILNFTLTGKKVGVIDLTN
                     GELSTRGTVASRKKETLAASRLMGIHVRENLGISDGNILLNEINKNKIIAVIRKYRPE
                     IIFAPYPFDRHPDHIHAGNLIKDCIFYSGLKKIITGKFKEYRPKRVFFYKSASDMPVS
                     FIIDVSKTFAKKLELLKCYSTQFYDPKSKEPETFISSKLFDKEIETRARYFGFKIGVE
                     FGEPYYSYEPLNIAPDTMFEI"
     gene            10170..12269
                     /locus_tag="PLX80_08815"
     CDS             10170..12269
                     /locus_tag="PLX80_08815"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQY20928.1"
                     /translation="MKTIKFFIFCFTIILSLILNGCSEDSNVDPVTPTPPIGSSIGSV
                     TANPDIMGVNTPTGITFKFTSGSGITINDSLVTLMKSDASGNFTIPSGMLQDNGNLTN
                     GDEIAGDGIFSGIISFTETSTGKIRFAAAANASASGTSFAGTSAADTVTVFESLSNDA
                     VTLVNTTLLAGQNKFITTLAGNTANYNTAMTETVSFLQSQFGVTTVNRGDANSTSLEI
                     NFVSGLKGCLTFSLQDANGSVQTRGGFPEDSLRSMRSRNESIPPERQTIGENSNVPQY
                     MTRTNSDNVNLDPMTVGNRNVLIFAPYEAAFAPFNERTKIVTNLTAGQCRGFQFTELV
                     NQNATVKSILDMTKYGLIVLATHGVQGKWFYTAEIVDTTSPDYLSLYKVLLTAGRLAI
                     ANNLVISTTGGVSTSATVYAIHHTFVSNLTGTFSNTVVLNNSCEGTMNNDLENAFKSK
                     GAKTYYGYTKVVNSGFCVTVADTVSRRYSRGLNSGQAFFNATDPVTPFAVFQIKGAND
                     MSFSLSLINGDFETGTLEGYTKDGDGRVITRLGTVNPTQASYMGIISTGLGFTTSSGS
                     VSQCISITPTQTNLTLKWNFLSEEFLEFIGSQFQDFFEVSVTNSAGVKTVLFRKTIDE
                     IAAQFGATKTDPGTLINVSPGIVFDQGGIYMTDWQTLTLNVAAFQNQIITISFNAGDV
                     GDSIYDTAILLDQISVQ"
BASE COUNT         4124 a         2110 c         2159 g         3941 t
ORIGIN      
        1 cgggacggcg tatatcaata tccgccacac gaactgcccg tcgagcgcac aatgcgggtt
       61 ctacctcaag aactactgat cctgcagcag ttcgcgtcca gggacccccg gccacgtggc
      121 cgggggttcg tcgttcaggc ttatccgtca aagggaattt agagaatggg caagggtgtg
      181 aataaccggt tgtgcgccgc gctgctcgta tcggtgctgg gcagcgcacc gctgcaagcg
      241 gccaacgagg gcgacgaggt gctgatccgg cgcgacggcg taacggtgac ccgtgccgat
      301 ttcctgcagc tgctcgacta cgcggtgccg caggagaagc gggcggctgt gctggccgac
      361 gagaataaga tccgcgagat gagagataag tattacaata tttcattcca gtatagcgga
      421 aagatcaact ggcatatggt cgggcattta cagtcaaaca aagttaaaga tattatagct
      481 ttcattcatc tgattcattc cgtagatacc ttcaatcttg ctgaagtaat tgactctacc
      541 gctaaaagga tcaaccgtaa aatagatatc cttgttcagg taaatacaag taatgaagaa
      601 cagaaatatg gtgtattacc tgaagaagct gaaagtctgt gcaaacaaat ttcattgctt
      661 gaaaatatta atgtgaaagg tctgatgaca attgcaagat taacggacga cagagacgag
      721 atcagaagaa gttttagaac actcagggaa ttgttcgata aaatgaaatc agacttcagc
      781 gattttaaat atttatccat gggaatgaca aatgattttg atattgcgat agaagaaggt
      841 tcaaatatgc tcaggatcgg cagcgctatc tttggagaga gaccggtcat agttgagtaa
      901 ctatgattca actggtcacg gatgataaga tttgaaatat caaatttaaa tcaaaaatta
      961 atttttttaa acaattataa taatgagtga caagatactt ccaaaagaaa taaagaaaac
     1021 tgatttcaaa aaatctttcc gtggatatga atctgctgaa gtagatgcat ttctggagac
     1081 tgttagtctt agatatgaaa gactttatga agagaatgtt tctcttttag agcaaataaa
     1141 aaatttaaat tcggatcttc agatttataa agagaacgag tcaacacttc agaaagcgat
     1201 tgtaaaatct caggatctca ccgaagagat aattgataca gcaaagaaaa aatctgagaa
     1261 tattataaag gaagctgaac taaattccca aaagatcctg caggattccg ataaagagat
     1321 catgacaaga aagcatgaac ttgaggaatt aaaattaaga aatgataaac ttgtagaaga
     1381 tgtaaaactt ttctttatgg aaaagctgaa tgagatggat gaatttgtaa aaagccggaa
     1441 tatctataaa atggagcttt ctaagcatga tcatgctgaa gattctgaag aagaacttcc
     1501 taaagaagaa atgcccgcta ttataaaaaa aatttccatt aatacagcta actctgactt
     1561 ttcggaataa cacttcctat ttatgaccga taaacatctt gaagcattat cacatttaac
     1621 taatcaattc gggaacagac ctgagacagg aataatactt ggtacagggc ttggcggact
     1681 ggtaaaatac attgacatag atttagaagt agaatactct gatataccta attttccggt
     1741 ttcaactgta gaatcacatt caggaaaatt aatttttgga cgtctatccg gaaaaaatat
     1801 tgtagctatg cagggaagat ttcatttcta tgaaggatat tcaatggatg aagttacatt
     1861 tccgatttac attttaaaat cattaggcgt aaaaaatctg attgtgtcaa atgcatgcgg
     1921 agctttgaat cagaaattta acaaggctga tctgatgata atgacatctc atattaatct
     1981 tttgtttgat actccattaa aaagaaagaa gctaaactac actgacggca gcaagatcta
     2041 ttccaaaaga cttattgata tggctgaatc gactgcaata gaaagtggta tcagcattca
     2101 gaaaggagtc tatgcaccgg tacagggacc gaatctggaa acagtttctg aatacagaat
     2161 gatcagaaaa atgggtggag atactgtagg catgtccact attcctgaag ctctcattgc
     2221 aagcaatctg ggaatggaag tattaggact ttcaattatt actgacaaag gatttcctga
     2281 tactttagaa gtagctgtac tgaaagacat tcttgaatca gcaagtattg ccgaaccaaa
     2341 attaacatta ctaataaaaa atctggcagc taagttataa tatttttaca aattattttc
     2401 acatttaaat aaagagtttt aaataagaga aatgaccgaa caatccgaaa aaatcaacca
     2461 gtctgcagaa tatattaaaa gcagattccc taaaaaatac aagcctgaac tttgtataat
     2521 tacagataat ttccaggaga tagcaaaaga atttaaatca tcgggtgact taaaatatat
     2581 tgatataccc ggatttaaga cttcgaatga caaagaaaat tcaggcaaaa taatatttgc
     2641 aaagagtaag aaaaaggatg tactgatatt ttcaggaaga ccgcattact atgacgggac
     2701 ggacatgaga tcgattggtc acagaatata cactttgaaa tttctaggaa taaaaaaaat
     2761 tatttcaatt tctgagactg cccatttaaa tccaagattt agctgcggcg aacttgctgt
     2821 tatttttgat cacataaatt taatgggtga caatccgctg attgggaaaa atgatgatga
     2881 acttggattg agatttcctg acatgagtaa cgcttataat aaagatctat acaaatcgtt
     2941 gtattcaata tttcagaata ataagatcag gataaatgaa tctgttttac ttggcatcac
     3001 aggtcctgaa agcgagactg aagcagaagc ccggttttac agggaaatcg gtgccgatgt
     3061 gcttggatat tcggtagtgc cggaaaacat aactgccgtt cactgcagaa ttgaatttgc
     3121 agctatcgga cttatttcaa gagagcttat tgcagataaa atgcttgatg acgttacatc
     3181 tgaaaaacaa aagataaaag atcaatcggg acatctcggc agtgctgtca aattattaaa
     3241 gggaattatt tcagaaattg ctgataagat ataaacatac aatccatgct ggatcggagg
     3301 aacttgaatt ttttgctgca gacttttgac ctgaaaagat taaagaatct caatcaaaaa
     3361 tccgttttaa tttcatataa aatttaatag atttataact caaaaaatca aacagctttt
     3421 atgcaggaca gattagaaat tccaaaaatg tataaattat tcatcggtgg taaattcaca
     3481 cgcactgaat ccgagaggta ttttgatgca ttaaatctga aagacggaaa aaagatatgc
     3541 aacatttcaa gagcatcgag aaaagatatt cgcaatagtg tagtagctgc cagatcagga
     3601 tttaatgact ggagcgggaa aacggcttac gaacgcggac agatcatgta cagatttgct
     3661 gaaatgcttg aaggcaggaa ggaacagttc attgatgaaa tttttatttc tactaaagag
     3721 tcaaaaagcg attgcaaaaa agaggttcaa aaagctgtgg acagattaat ttattttgcc
     3781 ggattttgtg acaagtggac tcaattatcc gggtcggtaa atccagttca gtcgggatat
     3841 tttaattttt ccattcctga gccaaccggt atagtcggaa taattcttcc tgatacattg
     3901 agttttcttc cattgatctc gagagtatct gcagtattaa gctcaggaaa ttcgtgtgtg
     3961 atcattgcaa atgaatcttc accacttccg gcattatctg tggcagaagt aattgctacc
     4021 agtgattttc cgtctggagt gataaatatt ctcacaggtt taaaaagtga attgaattct
     4081 catctcgccg gacattttga tgtaaatgct attgatcttt gtacaggaat cagatcagac
     4141 aaaaaaacca tacaggaact ttgcgcaaat aacgtaaaga gattttcttc aattcctgaa
     4201 aatcatgact ggtatagtga caaagacaat gagaatctga aagagattga aaaatttact
     4261 gagttaaaaa ctgtatggca tactatggga atgtaaatgg gaatgtaaat gggaatgtaa
     4321 attgagattc tgaatatcag gaaacatttt ttacaaaatt gaatttttaa gaacaaaata
     4381 tttttaaagg agtaaaaggt acttcagaat tatctggaaa attaatcata acatttaaat
     4441 gtccaacatc actaaactca taatcagcat tgctctttgt cagatggcag ggattattgg
     4501 gactttcttc acaatggatt caatacccgg ttggtatgag ggattaacaa aaccaagttt
     4561 taatcctccg aactggatct tcggtcctgt gtggattctt ttatatactt taatgggagt
     4621 ttcattattt ttagtgtgga aagaaggact tcagaatgcg gatgtaaaaa aagcatttta
     4681 tatattcatg attcagttgg tattaaattc tgcatggtca atagtatttt ttggaatgga
     4741 gtcaacttca ggaggactta ttgtaatatt tctattatgg attatgatcc tggttactat
     4801 ttataatttt ttaaaaatat caaaagcatc cggacttctt ctcttaccat attttctctg
     4861 ggtgtcattt gcaacattgc taaactattt tattttcaaa cttaactgaa taatttttaa
     4921 tgatattatt tagaaataca tgggatagat ttcaaagctt tttatttatc cttatagcta
     4981 ttctcatttc atcaaatgca tttgcagaag aaaacttatt aaaagactct gtaaatatca
     5041 gcggaaaaat taattccgaa aagatgtttt ttgaaacaac tgatatttcc agttcagatg
     5101 atatgataat ttaccatatg aaagaaaaat ttccaattat atctgaatct gatcttaaca
     5161 aaataatttc cactaaatca gttatttcca tagctgatcc cgacacaatt accggtattc
     5221 aaactgagga aaaattcaaa gtttcatttt atgtacccga aaatttcctt gtcgattcta
     5281 ctaaaatttt ctgggaacta aacattcctg aatttcagtc aaaaatttat cagctttata
     5341 atggtcaaaa tatttacata gatacctggc caaatgtagt aggtacaatg aaagacaaaa
     5401 cttatacagg taattttgaa gtataccgtg tgcggaatta tccgttctat aaagatcccg
     5461 atccaaagaa agctcactta agtccgacac ctccgggtaa aggaaatccg cttggattgt
     5521 ttgtagttca ttatgatgaa aattcccttc ggtattttca tgggaacaac aacaataagc
     5581 tgctctactc aaagatgaga aatctatcac atggttgtgt aagaaatgac aatgataata
     5641 ttgaagagat gaagcagttc ataattaaga aagtaataaa atccgaggat ctctccggat
     5701 ggctggacag taaaaagact ttggtttata atttagaaga aagcgataaa tttcctgtaa
     5761 agataattta taagacttat gaaatagata aagacaactc aggatattat gtcacattgt
     5821 ttaaagatat ttacaattat tcatcaggca ggataaatga aactctgaat gatccgggtc
     5881 tgatcttttt atcaaacaat gaaaaccttt ttgcagaata caggaaagta gttgggaatg
     5941 atattagtga tgatgctttg aatattctgg ttgaatatgt tttaaacaaa ggaattgaat
     6001 atgaaaaact ttacatttct gatcttaaaa attcattgag tatcagcaat taaaacgttt
     6061 catcccgcca gcggattatt ttccagacat cattggtgga aggtcttact agattcatat
     6121 cagcaaatcc gttaagtcgg actacgtcac tgggattgaa agtaatcgtc agattaaaac
     6181 ttctcttaac atttctgaca agagaatctc cgccttcgaa aattatatta ttccagacta
     6241 cttcgagctt ttgagcattc tgaaaaaggc cgtttgtagt tctcatctct gtcgctctgt
     6301 cccatgaaat atcaaacccg gaattataat ctctgtaaat aaacaggaat tgttcgccaa
     6361 gtaaatttcc ataaacagtt gtatcttcaa aggtatatgc atatttgaaa ttctgaaaaa
     6421 ctccttcaac agttttctga tcagtgatta tagaggttga tgtgctgagg tctaatttag
     6481 gtgaaaaaat attttcgcat gaagcaaaag aaataagcgt aacgataatt gcaatcggga
     6541 aaaaatattt aaatgcagta tttaatttta tgtgactcat tttttagtta aacatatttg
     6601 ctttaagcac actccatgtt gtatcaccat tatcattgat aaaatcactc cagctatgaa
     6661 tagaccacag acttcttaca tccggactca taacgaatct caatcttcct tttgtgacct
     6721 gtgcaatatt ttgtctgttg tgattaaata caagtatata gtccgaatca ataactgctg
     6781 aatcaattcc ggtattgaaa tttatattgg acaagaatag atttgtagaa gaattttctt
     6841 ctgtggaaga aatgagattg ctgaaataaa tacgctcact gcttaaattc cagttaagaa
     6901 aaacaggata cgcagtttgt gatactgcat caggaataaa tttaaatctc tttgttgaaa
     6961 aatttgaatc cacgagacat ctcatataat tatcaagatt tttttcggta acggcaaaat
     7021 taagattggc gagaactatg tcaggggaag taggctgagt gaatgttgat ctgttttctg
     7081 taggcggttc aacacttctc gtctcgaata gaccgcagcc gctgtaaaga ataaagatta
     7141 agatcagtat tttgtcaggt ctttttagca attattatta gccgttttga ttttttttca
     7201 tcgaatttat ttccgtagta atctccgaac aatgttttta ctttaaagtt aaattcccca
     7261 aagcatttta ccagttcttt tctgttatac agcttcaaaa tttctttgaa atttctaacc
     7321 tcagtatctt ttttaacttc aatatctttt ataataaacc cgttctcgat ccttcttttc
     7381 tgaatcactt taatttcgcc aaacttattt cggctgagag ggacaatgtt atttttaatg
     7441 aattctgaat taataaaatc aaaaacaaag tatcctcctt ttttaagtga atctgaaata
     7501 ttttttatca cactaaaatt ttcttcgtca ttatcaaaat atccaaaact tgtaaaaata
     7561 tttactgcaa ggtcaaagga atttttaaaa ttaaattttc tcatatccct tataaggaac
     7621 tttactttaa gatcattttc ctttgcttct ttgtgagctt ttttagcttc actaatcaaa
     7681 aattcagaaa gatcaaatcc ggttacatga aatcccctct tcgccaactg aagtgaatgt
     7741 cttccggcac cgcatgcgac atctaatact ttagcaccag tgatgatatc aatatttctc
     7801 tgaatgagat ctatcatatg acccgcttcc tcttcattcc tgtgactata aatatcgaga
     7861 tacaatttat cggaaaacca ttccttatac cacattgaca attttaaata ttaaatttta
     7921 aatattaaat taagatcctt cctcattgtt tccgaagagt catttgcttt acggactctg
     7981 cagttataga attaaattaa gatccttcct tctaaagctc ttgcgagagt aacttcatct
     8041 gcaaattcca gatctgatcc gacaggaatt cctcttgcaa ttcttgtgat cttcaactca
     8101 agtggtttca gaagtttatt cagataaagg attgtagttt caccttccac tgtaggattc
     8161 agtgcaagaa taagttcttc gactttatcg tcaacattgc tgccaagtct ttccagcaat
     8221 tctttgatcc ggatatcgtt tggaccaata ccgtccagcg gagaaatgat cccgtgaagc
     8281 acatgatatt tacctttgaa ttcattagtc ttttcaatag caaaaacatc ctgcggctct
     8341 tcgaccacac agatcatatt tcctttacgg ctgccagatc tgcagatatt acaaatttca
     8401 tcttcagtaa tattacaaca cacactgcaa aacctgagct tctctttgat ctccgtcaat
     8461 gctctggaaa atctttccac gtcttcacga ggctgccttg ttatatgcat cgcaagacgg
     8521 attgcagtct tcctgcctat ctgcggaagt cttgcaaatt catccacaag attttccaaa
     8581 gcatttgaaa tatttaccat ttggttaaaa tattataaaa ttttttattg aacgaaattc
     8641 ttttacgata gaaccattaa tcctaaaatc aaaaattttc acatacctga aatttgttaa
     8701 acccgaactg gaatattaaa ggtatttaga aaaactttaa tgcccttgtg gaaatagtaa
     8761 ttccttttat tagaacttag gaagattcat tccgggaata ttcggcatca ttcctgaggt
     8821 agcttttgac atttcttcat ctgccatttt ttgagagctc tccagccctt tatttacagc
     8881 agcaatgatc agatcttcaa gcatctgagg atcttccgga ttgattattt ctttttcaat
     8941 ttcaattttt gtgattctgt ttttgccatt cactgtcact tttaccattc ctccacctga
     9001 ttcttcagta acggatttgc cttccagctc ttcctgtatc ttatccattt tatcctgcat
     9061 cttttttatc tgtttcatca ttccctgcat ttgaccctga ttcattttta aatctccttg
     9121 atttatattt ttaaattatt ttttaattaa atattatttt attgatgcta cttttaaatc
     9181 tcaaacattg tatcaggagc gatattcaac ggctcatagc tgtaataagg ttcaccgaat
     9241 tccacaccaa tcttaaagcc aaaatatctt gcacgtgttt caatctcttt gtcaaaaagt
     9301 tttgagctga taaatgtctc cggctctttg cttttcggat catagaattg tgtcgaataa
     9361 catttaagta attcgagctt ttttgcaaat gttttactca cgtctataat aaaacttacc
     9421 ggcatatcag aagcactttt ataaaagaaa actcttttgg gtctgtactc tttgaattta
     9481 cccgttataa tttttttcag tcccgaataa aagatacagt ctttgatcag attacctgca
     9541 tgtatatgat ccggatgtct gtcaaatgga taaggagcga atattatctc aggacggtat
     9601 tttctgatta cagcaataat tttattttta ttaatttcat ttaataaaat atttccatca
     9661 ctgataccaa gattttccct tacatgaatt cccattagtc tggaagctgc aagtgtctct
     9721 tttttacggg aagcaacggt tcctctggta ctcaattcac catttgtaag atctataact
     9781 ccgacttttt taccggtcaa agtaaaattc aagatagtac caccgcaatt caactcagca
     9841 tcatctggat gagcagcaaa aaataatacg tcgagttttt tttcgggatt aatatttaaa
     9901 gatctatttt tgataggtct aatataatta ttataagaat tgaattaaat tgatttttca
     9961 tgaataaaat ttacaaattg atttttagtt tatatagaaa ataaaaattt tcttattaaa
    10021 aatttcaatg atcctaaatt catttaaatt gatttaattg tcaccatact tacatacaaa
    10081 aaattccgct tcatgtttaa attatagaat cattaatcag taattaattt tgtaatttgg
    10141 tccagttaaa aactaaattc aaacttaaga tgaaaactat taaatttttt attttttgtt
    10201 ttacaataat tttatcttta atattaaatg gttgcagtga agattcaaat gtagatccgg
    10261 taactccaac acctcctatt ggaagttcaa tcggatcagt aaccgcaaat cccgatatca
    10321 tgggagtcaa cacacctaca ggcattacct ttaaatttac atccggatca ggaataacaa
    10381 ttaatgattc acttgttaca ttaatgaaat ctgacgcatc cggtaacttt actattccat
    10441 caggaatgtt acaggataat ggtaatctta ctaacggaga tgaaattgcg ggagacggaa
    10501 tattcagcgg tataatttca tttactgaaa cctctacagg caagatcaga tttgcagctg
    10561 cagcaaatgc atcagcaagc ggtacttcat ttgcaggaac ttctgcagcc gacacagtaa
    10621 ctgtttttga aagtctaagc aatgacgcag taacccttgt aaatactact ttacttgcag
    10681 gacagaataa attcattact acacttgccg gtaacacagc gaattacaat actgcaatga
    10741 ctgagactgt ttcattccta caatcacaat ttggtgttac tactgtaaac cgcggagatg
    10801 caaatagtac aagcttagaa atcaattttg tctccggtct taaaggttgt cttacttttt
    10861 cattacagga tgcaaacgga tctgttcaga ccagaggagg ttttcctgaa gattctttaa
    10921 ggtcaatgag atccagaaat gaatccatcc caccggaaag acagactatt ggagagaaca
    10981 gtaacgttcc tcaatatatg acgagaacaa actccgataa tgtaaatctc gatcctatga
    11041 cagtcggcaa cagaaacgtt ttgatatttg caccatatga agctgccttt gcacctttta
    11101 atgaaagaac aaaaatagta actaacctta ctgcaggtca gtgcagagga tttcaattta
    11161 ctgaacttgt gaatcagaat gcaactgtaa aatcaatttt ggatatgact aaatacggat
    11221 tgatagtact ggctactcac ggtgttcagg gtaaatggtt ttatactgct gaaatagtgg
    11281 atactacttc tcctgattat ttaagtctgt ataaagtttt attaactgca ggaagacttg
    11341 caatagcaaa caatcttgta atttctacaa ccggtggtgt aagtacaagt gctactgttt
    11401 atgctataca tcacacattt gtatccaatc tgacggggac attttccaat actgtagttc
    11461 tgaataattc atgtgaaggc acaatgaata atgatcttga aaatgcattc aaaagcaaag
    11521 gcgcaaagac atattatgga tatacaaaag ttgtgaacag cggattttgt gtaacagtag
    11581 ctgatacagt ttcaagaaga tacagcagag gattaaattc aggacaggct tttttcaatg
    11641 caacagatcc ggtaactcca tttgcagtat ttcagatcaa aggtgcaaat gatatgtcat
    11701 tctctttgag tctgattaac ggagattttg aaaccggtac acttgaaggt tatacaaaag
    11761 acggagacgg cagagtgatc acaagactcg gcactgtcaa tccaactcag gcttcatata
    11821 tgggaataat ttctaccgga cttggattta caacttccag cggatctgtt tcacaatgta
    11881 tttccattac accgactcag actaatctaa cactaaagtg gaatttccta tcagaagaat
    11941 ttcttgaatt tatcggttca cagtttcagg acttttttga agtctcagta acaaattccg
    12001 caggggttaa aactgttcta ttcagaaaaa caatagacga gatcgctgct cagtttggtg
    12061 cgactaaaac tgatccgggt actcttataa atgtatctcc cggaatagtt tttgatcagg
    12121 gcggaatcta tatgactgac tggcagacac ttactctcaa tgtagctgca tttcaaaatc
    12181 agattattac aattagtttt aatgcaggtg atgtaggtga cagcatttat gatacagcta
    12241 ttttacttga tcaaatttca gttcaataaa tattttcttt tttcaaaaag tccatttcgg
    12301 ttttcgggat ggactttttt tataaccttt tttt
//
LOCUS       DAORVQ010000095        12317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2472_length_12317_cov_4.734301, whole genome shotgun sequence.
ACCESSION   DAORVQ010000095 DAORVQ010000000
VERSION     DAORVQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12317
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_12317_cov_4.734301"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..75)
                     /locus_tag="PLX80_08820"
     tRNA            complement(2..75)
                     /locus_tag="PLX80_08820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Pro,seq:ggg)
     gene            complement(197..628)
                     /locus_tag="PLX80_08825"
     CDS             complement(197..628)
                     /locus_tag="PLX80_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQY20929.1"
                     /translation="MITIFNAMLLTTLIFFSACSSAGNNNSNSAFYETRWVLRAFSDS
                     KVFTPDSKEEPFVFFSKTGGKATGFGGCNDFSFIFNKQKQKLKFENIVATEKFCESFM
                     ELEKNYLNIFVNSDSYKIIDDQLYLYKDSKLLARFENQNEK"
     gene            complement(644..1288)
                     /gene="pdxH"
                     /locus_tag="PLX80_08830"
     CDS             complement(644..1288)
                     /gene="pdxH"
                     /locus_tag="PLX80_08830"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQY20930.1"
                     /translation="MDKKNLFNLRKEFTIRELSEDNLKSNPFEQFDLWFSDALKIDSM
                     EANIMFVATSSSAGIPSLRTVLLKEFDENGFVFFTNYTSRKGKEIRENPNIAVLFYWN
                     ELERQVRIEGVCEKVSSEESVTYFNSRPFDSRISATVSEQSKIIENRDLLESKFSELK
                     KKYEDQNIPKPEHWGGYRLKPINFEFWQGRENRLHDRIQYSKDGDIWNINRLSP"
     gene            1816..3213
                     /gene="mgtE"
                     /locus_tag="PLX80_08835"
     CDS             1816..3213
                     /gene="mgtE"
                     /locus_tag="PLX80_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQY20931.1"
                     /translation="MSSDKKNLSVLEVDSELLDDITELIHEKSDAALLNILTDLYDFD
                     IAVIIDNLKNDDDSVYLFSLLNDDTASEVILEINDHRKDYITEKLSGEKISGIVSEMY
                     SDDATDFVSELDDDKKNEILENLDKIDKEDYDELKELLSYDENTAGGIMAKEYISVHE
                     NCTVMEAIKEIQDQADDVDQFYNLWVVDDNNYLKGILSLKRLIIALKEPDSLIRNVMN
                     SDVISIDANTDQEEVAKIFQSYDLVSAPVVDNNYKLIGKISIDDIVDVIEEEYSENVA
                     KMAGTDAEELEKKSPFEIAKLRLPWLLITLLIEFIAVFIVQLHNEIIEKIILIAAFMP
                     IISAISGNTGLQSAAIVVRGIDTGNISISKWWVPLIRQFKTNLLIGSAVALVVGLLGY
                     LMSDEYKFKFAASVGISMFISINIAGIIGTLSPMISKKLGFDPALTSGPFETAFQDVI
                     GIFIFMTVATFLLTY"
     gene            3355..4188
                     /locus_tag="PLX80_08840"
     CDS             3355..4188
                     /locus_tag="PLX80_08840"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQY20932.1"
                     /translation="MNLSLCILIIYFVSHYEKKNELEKRKRSWTRFLRFWYPSFMILF
                     CFKEIYLIMVGFDTRLYDPELIRIDQWLFGVNPTEFLYSFNNPFLVELLQIVYGLFYL
                     MPVIYAMELYLWHRYEELKYAIFVIFFGFYLSFLGYIIVPAIGPRFTLHEFANMNIEM
                     PGLYFAEIIRGLINFGESIPKNVPDPELYAQRDAFPSGHTIIIILITYLSHKIRSNSF
                     YFYLPFSILMIFSTVFLRYHYVIDLIAGIPFVIITILTANRIYKGKINFGRKLSKNIS
                     K"
     gene            complement(4169..5287)
                     /gene="hemW"
                     /locus_tag="PLX80_08845"
     CDS             complement(4169..5287)
                     /gene="hemW"
                     /locus_tag="PLX80_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQY20933.1"
                     /translation="MSGIYIHIPFCRKRCNYCDFFFITNTKLKSEYLSALKKEIVLHS
                     DSYKNEKFDTIFFGGGTPSVLTDVEVSEVISILRNNFNISDSSEITLESNPEDLENDA
                     SEKYFNAGISRFSFGVQSFIESELKFLTRQHTSESAERVIRKAAESTKNISVDIIYSL
                     PSQNPEDVIFSISKAIDSGANHISAYSLTYEKGTLLYKSLEKNPGIKNNSERDSELFK
                     TVSEKLLSEGFMHYEVSNFARPGFESRHNLKYWTYENYLGLGPSAHSFFNKRRWNNPG
                     SYSKYINYLNVNKLPLEEEYYPDRDQMKLEFIMLGLRSTGINLKKYKLFFGNEFLIDY
                     KDAAQELIDNGFAELSETSFKLNESGYSVADEITSRYF"
     gene            complement(5365..6324)
                     /gene="lepB"
                     /locus_tag="PLX80_08850"
     CDS             complement(5365..6324)
                     /gene="lepB"
                     /locus_tag="PLX80_08850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQY20934.1"
                     /translation="MNTYNKQGNKDSDKNSSKSTGTNSGKNDNEDKVKKSKPREFADA
                     LIYAGIVAFIIKILLFEAFRIPTGSMEKTLWVGDFLLVTKFTYGATTPRNIPFTDIRI
                     PYLKLPGFKDPKKGDIIVFDFPGDRDDLESTEVVNYIKRCVATPGDVLEIKDKKLYVN
                     GEIVPNPPNAVFDKVNRPESFFNPRMFPKGEMWNEDWYGPIRVPKIGDKIKIDTSTYE
                     GWKMFVMKEGHSIRMSGDGAVFVDEKELPNGEYTVEKDYLFMMGDNRNNSLDSRFWGF
                     MPVENIVGEALIIYWSWDPSISFLNFFDLLGTTRWERIGNIIR"
     gene            complement(6371..8170)
                     /gene="lepA"
                     /locus_tag="PLX80_08855"
     CDS             complement(6371..8170)
                     /gene="lepA"
                     /locus_tag="PLX80_08855"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQY20935.1"
                     /translation="MTKSNIRNFCIIAHIDHGKSTLADRLLEKTGTIEKRNMMKQVLD
                     DMDLEQERGITIKLHAIQMKYKSPDGEIYTLNLIDTPGHVDFSYEVSRSLAACEGALL
                     VVDATQGVEAQTISNLYLAIDNGLEIIPVINKIDLQNSMVDEVTDQIIELIGCKKEDI
                     ILASAKTDIGTEDILESIVHRIPAAKIETEKPLRALIFDSKFDSYRGVVVYIRVFEGT
                     LKQGDKIKSFFNEKVFEAEEVGNLRMGRSKTGQLNSGDVGYLIAGIKDVNDSKVGDTI
                     YKTSQPIEEPLPGYKEVKPMVFSGIYPTSTEDFEDLRDSLSKLKLNDAALTFEPESSV
                     ALGYGFRCGFLGMLHLEIIQERLDREYNMNIISTVPNVEFKVYTTKGEVIVVDNPSLM
                     PDPAKIDYIEEPYITANIITPNEFIGNLMKLANERRGVFIATNYVDAKRVDLHIDFPL
                     SEIVFDFYDKLKSYSRGYASFDYDFKDFRESKLVKLDILLNGEPVDALSTIVHKDKAF
                     EWGKKLCTKLKQLIPKQMYDVAIQAAIGSKVVARESISAIRKNVTAKCYGGDISRKRK
                     LLEKQKEGKKRMKQIGSVEVPQEAFHAVLSLDS"
     gene            complement(8315..8500)
                     /locus_tag="PLX80_08860"
     CDS             complement(8315..8500)
                     /locus_tag="PLX80_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HQY20936.1"
                     /translation="MEKINDLHSILDEMKPDIEKFYEKGQNAAGTRLRKQLNELRKKA
                     AEIRKDIQDERVKRKSE"
     gene            complement(8682..8951)
                     /locus_tag="PLX80_08865"
     CDS             complement(8682..8951)
                     /locus_tag="PLX80_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20937.1"
                     /translation="MQKITGLALVVFMIGHYILMHYQPESGHTYEAVLARMQFSWYRI
                     IDLSFLVLGMYHGLNGVWNIFRDYKMKSLAKYSVLSGIIVSDWHL"
     gene            9431..9637
                     /locus_tag="PLX80_08870"
     CDS             9431..9637
                     /locus_tag="PLX80_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQY20938.1"
                     /translation="MKSSIGNICQTYRKKELFHFAENKAAHLLYFIVKNQSFTDGNKH
                     IAAFLFYGFLKEMDYYIQMTDLKK"
     gene            9802..10227
                     /locus_tag="PLX80_08875"
     CDS             9802..10227
                     /locus_tag="PLX80_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20939.1"
                     /translation="MKILKIKNIIPVLISFIIIFSGSFSIALMHYSCVSDGYTHMECD
                     TETEDACCEQAVCCEFPGIHEEMITKMDCCEIHAEKYEHSEPYLPIIKDSKVKLYSQY
                     YCNNTIIIKHSDQSEINSYQYNLNFSLRDVCTLNSSFRI"
     gene            10351..10734
                     /locus_tag="PLX80_08880"
     CDS             10351..10734
                     /locus_tag="PLX80_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20940.1"
                     /translation="MKTIIVTALLFIAFSFSQNSNTLANNQDSVKTCIVSGEELSADV
                     VTYVYFNNTVELCCDVCLKAFKKEPAKYLAGNLWCPVCQDDDGKKDISTAHDNVKYYF
                     CNDGCKSTFESDPEKILSENKKDDE"
     gene            10709..>12317
                     /locus_tag="PLX80_08885"
     CDS             10709..>12317
                     /locus_tag="PLX80_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20941.1"
                     /translation="MKIKKMTNNIVLILLFISVLFSSESFSQNISGVVYGKINNKNET
                     LDAAIIKWINTKQGTFTDEKGKFEISAEGITDKRIIVSYIGFSADTVGVGNDLNLKII
                     LIPNSTTDEIIVNDDRGSSFIGRELPKTETITSQELVKDACCDLSGCFGRNSSVEVAV
                     TDILTDSKELKILGLEGVYTQILIDNVPLLTGLNVKYGVSSYPGTIIDRITVSKGSNS
                     VLQGYESISGIMNVILKNYSNSDKLMLNGYLNSMLEKQVNINYAEKFGNNLNSIFSFQ
                     SVQSSDRIDENGDGFLDNPLVTRYTFYNKWDYADDIKEVNRTDVNAAFRYWNEERTGG
                     QTDYFQDQDAGSDQIYGQTADLNSFEGYLRYSRQFNEDKSIKLYLNSSYYDQNSYFGF
                     TKYRGTQTSFTGSGFYETSFSDRTVLKTGLSYKYLNIDDNIVFEDTTSKTYPGDYPKL
                     ESIPGIFAEGSFNFLDDKASLMAGFRYDYHNVYKSIYTPRLLFRYQPEDAFVMRASIG
                     TGFRTINLFNEYNNILASSKDIIILEQL"
BASE COUNT         3963 a         2101 c         1910 g         4343 t
ORIGIN      
        1 gtcgggatgg gcagactcga actgccggcc tcacgccccc cagacgtgcg atctaaccaa
       61 ctgatctaca tcccgttcat tttactgtct gattatttct tagaatagct tgttcagtcc
      121 ttttttaatt gtgtagaaaa gacgttccaa tatgaagatt ttgtaaaatt aaatcaaacc
      181 tttaatctct gacaatttat ttttcatttt gattttcaaa tcttgcaagt agttttgaat
      241 ctttatacag ataaagctga tcatctatta ttttataaga atcagaattc acaaatatat
      301 ttaaataatt tttctccaat tccataaaag actcacaaaa cttttccgtt gcaacaatat
      361 tttcaaattt taatttttgt ttttgcttat tgaatataaa actgaaatca ttgcatcctc
      421 cgaatccggt agctttgcca cctgttttcg aaaaaaatac aaatggttct tccttactat
      481 caggtgtaaa tacttttgag tcactgaatg ctctgagtac ccatctggtc tcataaaaag
      541 ctgaattcga attattgttt cctgcagagg aacatgctga aaaaaatatt agtgtagtta
      601 aaagcatagc attgaaaatt gtgatcataa cagtagtttt taattaaggt gacaatctgt
      661 ttatattcca tatatcaccg tctttagaat attgtattct gtcatgaagc cggttttccc
      721 gaccctgcca aaattcaaag tttattggtt tcaatcgata tcctccccag tgttcaggtt
      781 taggtatgtt ctgatcttca tattttttct ttaattctga aaatttagat tcgagtaaat
      841 ctctgttttc aattatttta ctttgctcag aaacagtcgc tgatattctg ctgtcaaatg
      901 gtctggagtt aaaataagta acagattctt cagaagatac tttttcgcaa actccttcaa
      961 tacgcacctg tctctcaagt tcattccagt aaaaaaggac agctatatta ggattctctc
     1021 tgatctcttt accttttcta cttgtataat ttgtaaaaaa cacgaatcca ttttcatcaa
     1081 attcttttag aaggacggtt cgcagagacg gaataccagc tgaagaactt gttgcaacaa
     1141 acatgatatt tgcttccata gaatcgatct ttagtgcatc agaaaaccat agatcaaact
     1201 gttcgaaggg attacttttt aagttatctt cagataactc tcttatagta aattcttttc
     1261 gaaggttaaa taaatttttt ttatccataa taaaaaatta gttttcacaa gttagatatt
     1321 ttgagtttga gaattaatga cattaatttt attgttaaat aaattggaat agattgaaaa
     1381 ttttggatgt aaattctccg gaaatgttaa aaaaagaaat gcctgataaa agtaagaaat
     1441 tgaaatttta tatagaaatt ttaaattttt gagtatttca aaaaaggaat agtggaaaga
     1501 aatggtttta tggtaaaaga ttaacatttg cttgttcgga aaaaaccgtt taaacggttt
     1561 ttataccaag aggcaatttc gaaaattgaa aagtattata ttttctatag atcaattaat
     1621 agaaaaaata actttctgat gggaaaagtg aatttcataa ttaaattcat tacggcagat
     1681 aaaaaccgtt taaacggttt ttatccaaac ccaccaaacc tcccccaata ccatattcac
     1741 tctattcaat tgatttatct catctttata agtaatttgt taatcaaaat ttattaactc
     1801 tatttctgat ttcctttgtc ttccgataaa aaaaatttat ccgttctcga agttgacagc
     1861 gaattactcg atgatataac agaactcatc cacgagaaat ctgatgccgc tctgcttaat
     1921 attcttaccg atctctacga tttcgatatt gcagtcatta ttgataatct taaaaatgat
     1981 gatgattctg tttatctttt ttctctctta aatgacgata ctgcaagtga agtcatactt
     2041 gaaattaacg accacagaaa agattatatt actgaaaaat tatccggcga aaaaatatcc
     2101 ggtatcgtca gtgaaatgta ttcagatgat gctactgatt ttgtttctga gcttgatgat
     2161 gataagaaaa atgagattct tgaaaatctt gataaaattg ataaagaaga ttacgatgaa
     2221 cttaaagaac tcttaagcta tgatgaaaat actgccggcg gtatcatggc aaaggaatat
     2281 atttctgttc atgaaaactg tactgttatg gaagctatta aagaaattca ggatcaggcg
     2341 gatgacgtgg atcagtttta taacttatgg gtggttgacg ataataatta tcttaaaggt
     2401 attttatctc ttaaacgtct tatcattgct ttaaaagaac cggattcact gatacggaat
     2461 gtaatgaatt ctgatgtaat ttctatagat gcaaatactg atcaggaaga agttgctaaa
     2521 atttttcaaa gctatgacct tgtttcagca ccggtcgttg ataataatta taaattgatt
     2581 ggtaaaattt caatagatga tatcgttgat gttattgaag aagaatattc tgaaaatgta
     2641 gctaaaatgg caggtacaga tgctgaggaa ctcgagaaaa aatctccgtt cgaaattgct
     2701 aaactcagac tgccgtggtt attaattact ctactcatag aatttatagc agtttttatc
     2761 gtgcagctac ataatgaaat tatagaaaaa attattctga tcgctgcttt tatgccgatc
     2821 atttctgcga tttccggaaa tacaggtctt cagtctgcag ctattgtagt aagaggaata
     2881 gacacaggaa atatcagtat cagtaaatgg tgggtcccgc tcataagaca atttaaaaca
     2941 aatctactca taggttctgc tgtagctctc gttgttggat tactaggcta tttaatgagt
     3001 gatgaatata aatttaaatt cgctgcttca gttggaattt caatgttcat atctataaat
     3061 attgcaggga tcattggaac tttgtctcct atgatctcaa aaaaattagg ctttgatccg
     3121 gctcttactt ccggaccttt tgaaacagca tttcaggatg tgattggaat ttttattttt
     3181 atgacagtcg caactttttt actcacatat tagagtcaat tgtttagttc tttaaaaaaa
     3241 aattttagtt atctggaaat tcctgatgtt accaatcaga tatttcttgt attactctca
     3301 gtaattttta ttttctcatc ggataattta ctgctgtgca tcctaattcc ttttttgaat
     3361 ctatcgctct gcatactcat tatttacttt gtttcgcatt atgaaaagaa aaatgaatta
     3421 gaaaaaagaa aaagatcttg gacaagattt cttagatttt ggtatccttc ctttatgatt
     3481 cttttttgtt ttaaagaaat ttatctgata atggttggat ttgacaccag actctatgac
     3541 cctgagttga tcaggatcga tcaatggtta tttggtgtaa atccgacaga gtttttgtat
     3601 agtttcaata atcctttcct tgtcgaattg ttacagatcg tctatggatt attttattta
     3661 atgcctgtca tatatgctat ggagctttat ctctggcata gatatgaaga attaaagtat
     3721 gctatatttg taattttttt cggattctat ctttcctttc tcggatatat aattgttcct
     3781 gccattggtc ccagatttac tttgcatgaa tttgcgaata tgaatattga aatgcccggt
     3841 ttatattttg cggaaattat cagaggcctc ataaatttcg gtgaatccat tcctaagaat
     3901 gtacccgatc ctgaacttta tgctcagcga gatgcatttc cttcaggaca tacaattatt
     3961 attattctca ttacctatct ttctcataag atcagatcaa actcattcta tttctatctt
     4021 ccgttttcaa ttttaatgat cttctctacc gtttttctgc gttatcacta tgtcatagat
     4081 cttattgcag gtattccatt tgtcataata actatattaa ctgcgaatag aatttacaaa
     4141 gggaagataa attttggtag gaagctttct aaaaatatct cgaagtaatt tcatctgcaa
     4201 cagaataacc cgattcattt agcttaaaac ttgtttccga caattcagca aaaccgttat
     4261 ctatcagctc ctgtgctgca tctttataat ctattaaaaa ttcatttcca aaaaataatt
     4321 tgtacttctt taaatttatt cccgtacttc ttaatccaag cataataaat tctaatttca
     4381 tctgatctct gtctggataa tattcttctt ctaatggtaa tttatttaca ttcaggtagt
     4441 ttatatattt tgaatatgaa cctggattat tccatcgtct cttgttgaaa aaggaatgtg
     4501 ccgaaggacc cagtcccaga taattttcat atgtccagta tttcagatta tgtctgctct
     4561 caaaaccggg tctcgcaaaa tttgaaactt cataatgcat aaatccttct gacaataatt
     4621 tctcagaaac tgttttaaat agttcagagt ctctttcgga attgttcttt atgccgggat
     4681 ttttttctaa tgatttataa agaagtgttc ctttctcata agtaagtgaa tacgctgaaa
     4741 tatgatttgc tccggagtca atcgcttttg aaattgaaaa aattacatct tccggatttt
     4801 gagaggggag tgaataaatt atgtcaacac tgatattctt agtactttca gcagctttgc
     4861 gaatgactct ttctgcagat tcggaagtat gctgccttgt gagaaattta agttcacttt
     4921 cgataaatga ctgtactcca aagctgaatc tgctgatccc tgcattaaaa tatttttccg
     4981 aagcatcatt ttcaagatct tcaggattgg attcaagtgt gatctcagat gaatcactaa
     5041 tgttgaaatt atttcggagt atgcttataa cttcagatac ttctacgtca gttaaaacag
     5101 aaggcgtacc tcctccaaaa aatattgtat caaacttttc gtttttataa ctgtcagaat
     5161 gaagcacaat ttcttttttt aaagccgata aatattctga tttgagttta gtatttgtaa
     5221 taaaaaagaa gtcacagtaa ttacatcgct ttctgcaaaa tggaatatga atataaatcc
     5281 ctgacattct tagtatttaa attgaaaatt ccgaagcctg attattgact tcggaatttt
     5341 tcattattaa aattttaata taaattatct gataatattt ccgattcttt cccatctcgt
     5401 agttcccagc aggtcaaaga aattcagaaa tgatatactt ggatcccatg accagtaaat
     5461 tatcaaagct tctccgacta tgttttctac aggcataaat ccccagaatc tgctgtcgag
     5521 tgagttgttt ctgttatctc ccatcataaa taaataatct ttttcaactg tatattctcc
     5581 gttcggcaat tccttttcat cgacaaacac agctccgtca ccagacattc ttattgaatg
     5641 accttctttc attacaaaca ttttccatcc ttcatatgtc gatgtatcga tcttaatttt
     5701 atcacctatt tttggaacac gtatcggacc ataccagtct tcattccaca tctcaccttt
     5761 gggaaacatc cttggattaa aaaaactttc cggtctgttt accttatcaa atacagcatt
     5821 tggaggattt ggaacgatct ctccgtttac ataaagcttt ttatccttta tttcaagtac
     5881 atctcctggt gtagcgacac atctttttat ataattaacg acttcagttg attcaagatc
     5941 atctctgtct cccggaaaat caaatacaat tatatcacct ttttttggat ctttaaatcc
     6001 gggtaatttc aaatatggaa ttctgatatc ggtaaatgga atattccttg gtgtagtcgc
     6061 accgtatgta aattttgtaa ctagtaaaaa atctcccacc cataaagtct tttccattga
     6121 tcctgtaggt attctgaatg cttcaaataa aagtatcttg attatgaatg caactatacc
     6181 tgcataaatt aatgcatcag caaattctcg tggcttggat ttttttactt tgtcttcatt
     6241 atcattcttt ccgctatttg ttcctgtgga cttcgaactg tttttatctg aatctttatt
     6301 tccctgttta ttataagtat tcacctaata aattttttga gtaatattct gtttaatttg
     6361 aatcgagttt ctaactgtca agtgaaagta ctgcatgaaa ggcttcctgc ggaacttcaa
     6421 ctgaaccgat ttgcttcata cgtttttttc cttccttttg tttttcaagt aattttctct
     6481 ttcttgaaat atctccgccg taacattttg cagttacgtt ctttctgatc gcactaatgg
     6541 attctcttgc taccactttt gatccaatag ctgcctgtat tgcaacatca tacatctgtt
     6601 ttggaatcaa ctgctttaac tttgtgcata attttttccc ccattcaaat gctttgtctt
     6661 tatgcacaat tgtcgataat gcatctaccg gttctccgtt aagaagtata tcaagcttta
     6721 caagcttgga ttctctgaaa tccttaaagt cataatcaaa agaagcataa cctctggaat
     6781 aagatttgag cttatcataa aaatcaaaaa caatttcact gagtggaaaa tcaatatgta
     6841 ggtcaactct ctttgcatct acataattag tggcaataaa tactcctctt ctttcatttg
     6901 caagtttcat cagatttcca atgaattcat ttggcgtaat aatatttgca gtaatgtacg
     6961 gttcttcaat ataatctatt tttgctggat ctggcattaa agaaggattg tccactacga
     7021 tcacttcacc ttttgttgta taaactttaa attcaacatt cggtactgta ctgataatat
     7081 tcatattata ttctctgtcc agtctctcct gaatgatctc aagatgaagc ataccaagaa
     7141 atccacatct gaatccataa ccaagtgcta ccgatgattc cggctcgaaa gtaagagcag
     7201 catcatttaa tttcaatttc gatagtgaat ccctcagatc ttcaaaatct tctgttgaag
     7261 ttggataaat tccgctgaat accataggct ttacttcttt atatccgggt aaaggctctt
     7321 ctataggctg tgaagtctta taaattgtat ctccgacttt tgaatcattt acatccttaa
     7381 ttcctgcaat taaatatcct acatcccccg aatttaactg accggtttta gatctgccca
     7441 ttctcagatt cccaacttct tccgcttcaa atactttttc attgaaaaaa gatttgatct
     7501 tatcaccctg tttcagagtt ccttcaaaca ctctaatata aacaacgact cctctgtaac
     7561 tgtcaaattt tgaatcaaag ataagtgctc tcagtggctt ttctgtttca atttttgcag
     7621 ccggtattct gtgaacaata ctttctaaaa tatcttcagt tccaatatct gtttttgcac
     7681 tggcaagaat tatatcttct ttcttacacc caataagttc gatgatctga tcagtaactt
     7741 catctaccat tgaattctgc aggtcaattt tattaataac aggaattatt tcaagaccgt
     7801 tgtcaattgc cagatacaga ttactgatcg tctgagcttc cacgccctga gttgcatcca
     7861 cgacaagtaa tgctccttca caggcagcaa gtgaccgtga aacttcatat gaaaaatcaa
     7921 catgaccggg agtatcgatc agattaagag tgtaaatctc tccgtcaggc gatttatatt
     7981 tcatctgaat agcatgaagc ttgatcgtga tccctctttc ctgctcgaga tccatgtcat
     8041 ctaagacttg cttcatcatg ttcctttttt ctattgtacc tgtcttttca aggagtctgt
     8101 cagcaagtgt ggatttcccg tgatctatat gcgcaattat gcagaaattt ctaatgttgg
     8161 attttgtcac taatgtattt aaaagtgtgt aaaataaaag tggattctaa aaagtaagtt
     8221 ataaaatgaa atataactta tttagattcc tctccttaaa taaaaataaa aattaaaaaa
     8281 gttaaaaaaa ttaataatat taagaacttt aatattattc agactttctt ttgactcttt
     8341 cgtcctgaat gtcttttctg atctctgcag ctttctttct aagttcattt agttgttttc
     8401 ttaatcttgt acctgctgca ttctgacctt tttcataaaa tttttcaata tcaggtttca
     8461 tttcatctaa gattgaatgt aaatcgttaa ttttttccat tttccggatc tcctcttagt
     8521 ttgttttgtt tgaataagtt aattaataaa tttggtaaaa gtatcaaaaa aatttaatat
     8581 ttgtaagtta aatttttaaa ttgatttaaa aattctgaac ctgtaaaaat acgaatttta
     8641 tgataaatat ggtatcgaaa agatttttgt aaatccccat gctacaaatg ccagtccgaa
     8701 acaattattc ctgaaagaac tgaatacttt gccaatgact tcattttgta atccctgaag
     8761 atattccaga ctccgtttaa tccgtgatac atccctaata caagaaatga aagatctata
     8821 attctgtacc atgaaaactg catccttgca agtactgcct catatgtatg acctgattca
     8881 ggttgataat gcattagaat gtagtgtccg atcataaata caaccaaagc aagacctgta
     8941 attttctgga ttacccagct tcctgtatct ttttacaact cagattttcg gatatccagt
     9001 ttttctgatc gaaggttttt aatttctgaa gtgtgttttt agaacccgaa tcactttcca
     9061 attaaacgga gctccttagt ttttaaagtt tcgttaaatt aattatttca gcttgtattt
     9121 acaatttatt aaaatttatt ataaattcag ggatttgtta gcaagaatat ttagttatga
     9181 ccgtctcagt gtgaataaca attgagaatt aaaaattaaa aattaagaat tttcaaaaac
     9241 attgtacatt gcaaattgaa aatttagaat ttaaaattaa aaattaagaa ttaaaaatat
     9301 caaaaacatt gtacattgca aattgtacat tgattattgt atacatgtgt tttttggact
     9361 ctgcatgtaa taacaatact tttaatgtat tgaatttgcc ggaacttttt ggcaaagata
     9421 agacgggtca ttgaaaagtt caattggaaa tatttgtcag acataccgga aaaaagaact
     9481 ctttcacttt gcagaaaata aagcagcaca tcttttatat ttcattgtaa aaaatcaatc
     9541 attcacagat ggaaataaac atatagcagc atttttgttt tatggtttct tgaaagaaat
     9601 ggattactat attcaaatga cggatctgaa aaaatagaag ataatacact tgctgctctt
     9661 actctaatga tagccgaatc aaatccaact gaaaagaaat gatggtcaga ttaattataa
     9721 atcttatgga aaaaaataaa aaataaatcc ggactttata aaagggtttt ttcttaacaa
     9781 cattatggtt atttttagct tgtgaaaatc cttaaaataa aaaatatcat tcctgtacta
     9841 atatcattca taattatctt ctcagggagt ttttcaattg cacttatgca ttattcctgt
     9901 gtcagtgatg gatacacaca tatggaatgt gatacagaga ctgaagatgc ctgttgtgaa
     9961 caagcagtct gctgtgaatt tccgggcatt catgaggaaa tgattactaa aatggattgc
    10021 tgtgaaattc acgcagaaaa atatgaacat tcagaaccat atcttcctat cattaaagat
    10081 tctaaagtaa aactatattc acaatactac tgcaataaca ctataattat aaaacattct
    10141 gatcagtcag aaatcaattc ttatcaatac aaccttaact tctcactcag agatgtctgc
    10201 actttaaact ccagtttcag aatttagact tacaaattaa tttcgcttat taactcaatt
    10261 tatttctaac aggaattatt ccctgtattt gaataattga tctatacctt tatatcaaaa
    10321 tttaaaaaac ttaaactata taaattaatt atgaaaacaa ttatcgtaac agctctttta
    10381 tttatcgctt tttcattttc acaaaacagt aatacacttg caaataatca ggattcagta
    10441 aagacatgca tcgtcagcgg tgaggaatta agcgcagatg tcgtaaccta tgtttacttt
    10501 aataatactg tcgagctttg ctgcgatgta tgcttaaaag cattcaaaaa agaaccggcc
    10561 aaatatcttg caggtaatct atggtgtccg gtatgtcagg atgatgacgg aaaaaaagat
    10621 ataagtaccg ctcatgataa tgtgaaatat tatttctgta atgacggatg taaaagtact
    10681 tttgaatcag acccggaaaa aatattaagt gaaaataaaa aagatgacga ataatatagt
    10741 cttaatatta ttatttattt ctgttctgtt ttcatctgaa tcattttctc aaaatatcag
    10801 cggtgtcgtg tatggtaaga tcaataataa aaatgaaact ctcgatgccg ctatcatcaa
    10861 gtggattaat acaaaacagg gaaccttcac agatgagaaa gggaaatttg aaatttctgc
    10921 cgaaggtatt actgataaaa gaataattgt ttcatatatt ggattctctg cagatacggt
    10981 cggggttggt aatgatttga atttgaaaat tattcttatt ccaaattcga ctactgatga
    11041 aataatagta aatgatgaca gaggctcttc atttatcgga cgtgaattac ccaaaactga
    11101 aactatcact tctcaggaat tagtaaagga tgcatgctgt gatctttcag gttgtttcgg
    11161 gagaaattcc tcagtcgaag tagctgtgac tgacattctt actgacagta aagaacttaa
    11221 aatactcgga ctcgaaggcg tttatactca gatactaata gataatgttc cgcttctgac
    11281 cggactgaat gtaaagtacg gtgtcagcag ttatcccggg acgatcattg acaggatcac
    11341 ggtttcaaaa ggatcgaact ctgtcctgca gggatacgaa tccatcagcg ggatcatgaa
    11401 tgtcattctg aaaaattaca gtaattctga taaacttatg ctcaatggat atctgaacag
    11461 tatgcttgag aaacaggtga atataaatta cgcagaaaaa ttcgggaata atctcaattc
    11521 tattttttca tttcagtcag ttcaaagctc tgacaggata gacgaaaacg gagatggctt
    11581 tcttgataat ccacttgtca cccgatatac tttttataat aaatgggatt atgctgatga
    11641 tataaaagag gtcaacagaa cagatgtaaa tgctgccttc cgttactgga atgaagaaag
    11701 gacaggagga cagacagatt attttcagga tcaggatgca ggcagtgatc agatctacgg
    11761 acagactgct gatctgaatt catttgaagg atatctccgt tacagcaggc agtttaatga
    11821 ggataaaagt atcaagctct atttgaactc atcctattat gatcagaatt catattttgg
    11881 atttacaaaa tacagaggaa ctcagacttc gttcaccgga tctggatttt acgaaacatc
    11941 tttctcggac agaacagttt tgaaaaccgg attaagttat aagtatttga atattgatga
    12001 taatatagta ttcgaagata caacttcaaa aacttaccct ggtgattatc caaaactgga
    12061 atcaataccc gggatctttg cggaaggttc tttcaatttt cttgatgaca aagcaagtct
    12121 catggcgggt ttcagatatg attatcacaa tgtctataaa tcaatttata caccgagact
    12181 gcttttcaga tatcagcctg aggatgcatt tgtaatgaga gcttccattg gaacgggatt
    12241 caggacaatt aatctgttca atgaatacaa caatatactg gctagctcaa aggacataat
    12301 aattctcgag cagcttg
//
LOCUS       DAORVQ010000096        12253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2491_length_12253_cov_5.262666, whole genome shotgun sequence.
ACCESSION   DAORVQ010000096 DAORVQ010000000
VERSION     DAORVQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2491_length_12253_cov_5.262666"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..764
                     /locus_tag="PLX80_08890"
     CDS             36..764
                     /locus_tag="PLX80_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY20942.1"
                     /translation="MFPIEFKNVSKFIRGRSILKNISLGFRENKITAVIGKSGSGKST
                     LLKCINGLIKPSEGEVILFGSSLDYENLIPVRMQIGYSVQGTGLFPHLNIFENISLLA
                     KLNDKRNEDIESRTKLLMEMTDLSLEHGRKFPYQLSGGEQQRAGLCRAMMMNPKIFLL
                     DEAFGALDVTTKNEVHLELLKIQKTEPRTIVLVTHDLNEAFKLADEIVIIEKGEIQQK
                     GNRDEILNSPANDFVKYFTASQLI"
     gene            761..2269
                     /locus_tag="PLX80_08895"
     CDS             761..2269
                     /locus_tag="PLX80_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQY20943.1"
                     /translation="MKILFTIILILFSFSKLNSQTSQTIKVGAKHFNEGYILSEILSQ
                     LLEKNGFEVERKFNLGGTLICYEALSNGDIDLYPEYTGTISEAILKMNEKVSFTELKK
                     ILNEKDLDISGEYGFNNTYALAVKNETAKTLSLENISGLKVHPELKLGLSYEFLKRED
                     GWMELSEVYQLKQKPVGLEHGLAYKALEDGQIMLTDVYSTDGEIPKYDLKILNDDKNF
                     FPKYYAVTFYKNKIADSIKNILNVLTGVISEKEMQEMNSKVLFENRTFSEVAGEFIKS
                     KDLFNDKPDLNTHDKESILSEIFAKLIQHLKLTFLALLISIVIAVPAGIFIYTYSSFS
                     KPVLYLAGLLQTIPSIALLAFMIPLFGIGFLPAVIALFLYGLLPILRNTTIALFSVDP
                     LLKEVAEGMGLSKKQKLFHIELPLSLPTILAGIRTAAVINIGTATLAAFIGAGGLGEF
                     IVTGLALNNTNLILMGAVPAALLAVLTEIFFEILEKYITPEYLKRTETINNL"
     gene            complement(2539..3294)
                     /locus_tag="PLX80_08900"
     CDS             complement(2539..3294)
                     /locus_tag="PLX80_08900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY20944.1"
                     /translation="MNFFKIYILITVFFSIFITNLSAQTNSSNNVCEKWNALDEKIKT
                     GKIDVEDAQELLISYEPAVIKYYKNNGGKVYKRSDWVFPLKNFTSIYYREGGNDFRLS
                     TYDYFQGSNSKGHPAHDIMILDENKDLLDDSTLKPVDVVSMSGGVVVATDTTWDVGSV
                     LRGGRYVKIFDVTNKGLFYYSHLSVVSVKPGDILAPGDKIGEVGRTGRKTILKEGKTH
                     LHVSYLISVEGYPVPEEFINDLRTSEKKVIKDH"
     gene            complement(3437..5677)
                     /locus_tag="PLX80_08905"
     CDS             complement(3437..5677)
                     /locus_tag="PLX80_08905"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQY20945.1"
                     /translation="MFDLELIKNVYADFKSKVEDAKKLLGRPMTYTEKILYAHLWEKP
                     TTPYNRGIDFTDFKPDRVAMQDATAQMALLQFMSAGMPKVAVPSTVHCDHLIEAEVGA
                     KNDLLRAYGDNKEVYDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMLIGTDSH
                     TPNAGGLGMIAIGVGGADAVDVMAGMPWELKFPKIIGVKLTGKLSGWTSPKDVILKLA
                     GILTVKGGTGAIVEYFGEGTSSISCTGKGTICNMGAEIGATTSLFPYDEKMFAYLKAT
                     EREEIGNLAYGISAELKADEGSESYYDQIIEIDLSTLEPHVNGPYTPDLAWPISKFKD
                     AVIENGYPEELKVALIGSCTNSSYEDMERSASVARQATAKNIKARSEFTITPGSEQIR
                     ATIERDGQLEAFEKVGGLVLANACGPCIGQWKRHDVKEGEKNSIITSYNRNFSKRNDG
                     NSATHSFVASPEIVTAFALAGNLTFNPLTDTIRNENGEEVLLDIPTGNELPDRGFLKG
                     EEGYVAPAEVGSDVEVVIHPESQRLEALEPFKMPDLENDFKNLQVLLKAKGKCTTDHI
                     SMAGPWLRYRGHLDNISNNMFIGAVNSFNDKVNEVKNHLSGNYEEVPRVARHYKGKNI
                     GWVVIGEENYGEGSSREHAAMEPRFLGGKAIIVKSFARIHETNLKKQGMLPLTFNDPA
                     DYDKIREDDKIDLDVSDIKPGIQIKMTLKHSDGTEENVMLNQTMNDQQIKWFQNGSAL
                     NIMSGG"
     gene            complement(5840..6598)
                     /locus_tag="PLX80_08910"
     CDS             complement(5840..6598)
                     /locus_tag="PLX80_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HQY20946.1"
                     /translation="MLDQIDLTKVMVLDVETVPQRKSYSELEDSWKALWEKKMKFKIP
                     EDKTGGDVYDQAGIYAEFGKIICISCGVFTKDGIFRIKSFYGDDEKILLEEFSEMLNN
                     NLNSDDNYLCAHNGKEFDYPYIARRCIINGLKIPAILDNSGKKPWEIKNLDTMDMWKF
                     GDYKSYTSLILLAGALGIKSPKDDIDGSDVWRIYWEENDLMRIVKYCQKDIVTVAQIL
                     LKFKGLELLKDDDIIFADEDADNENENAETETQE"
     gene            complement(6629..6985)
                     /locus_tag="PLX80_08915"
     CDS             complement(6629..6985)
                     /locus_tag="PLX80_08915"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQY20947.1"
                     /translation="MITKAETVSEFMEKIDADRKKDLTILRKIIKKTVPGIKESMKYG
                     MPTYDVNDKVILAFNSQKNYMSFYADSKSVEKNRDLLKVLNGVSCGKSCIRFTSIDKL
                     PEDVITKIIKDSDGVC"
     gene            complement(7008..9134)
                     /locus_tag="PLX80_08920"
     CDS             complement(7008..9134)
                     /locus_tag="PLX80_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQY20948.1"
                     /translation="MQSKLKSNKLLNEKSPYLLQHAYNPVDWHPWSEDTFEISRKEDK
                     PIFLSIGYSTCYWCHVMEREVFENEEIAKLINEFFINIKVDREERPDVDRVYMTALQA
                     LTGSGGWPMSMFLTPELKPFYGATYIPPKAKYGRAGFEDVITQINDAWENKRSDVMNS
                     GDNIVLKLQELINTRLSSDGDKTLSKEILENAFKNTMSIYDEIEGGFGSGNKFPRPVV
                     YNFLLDHYYHNKDFLALDAVTFSLKKMYEGGMYDHLEGGFHRYSVDNFWRVPHFEKML
                     YDQAQLISAYTDTYLVTKNKFYLYVAEDTAEYVLNILSDENGGFYSAEDAESAPDKNK
                     PDVKEEGAFNLWEKEEIEKILGKDNAEIFNYYYGILTNGNTVNDPHEVFGKKNVLYIA
                     NDIYETGKNFDKMPEEIARIIDDCRTELLAERSERSRPYLDDKILTSWNGLTISAFAK
                     LYQATGKEKYYDRALAAVDFILKNLYKPEDKVLLHRYRDGESRFNGTLEDYAFFISGL
                     INMYEAGYEIKHLTLAIELNKLVIEKFYDNSSAGFFDVERNEKDILLKTKDTYDGAEP
                     SGNSIQVGNLLKLGYMTDDQDLSDKADRCLNIFSEDLRRLPFSSPQMLCDLSFLLNTP
                     KEIIISGNKDTDEFKKLNEVICKEYIPNSVKLHAAEELGKISPFIENITGKKNESNKA
                     EVFVCENYKCELPVNDPEVLKEKLTK"
     gene            9578..10933
                     /locus_tag="PLX80_08925"
     CDS             9578..10933
                     /locus_tag="PLX80_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQY20949.1"
                     /translation="MEFKSVNPYSGKEAGIHKEHTEEELSEMLNKAGESFNSWRNVPI
                     VERSELMRNAGQVLRNNTEEYARMITLEMGKPITESRAEINKCAWVCDYYADNAEGFL
                     SDEIIVTDAQKSFVRHDPIGCVLAVMPWNFPFWQVFRFAAPTLTAGNTAMLKHSSNVF
                     GCAKQIEDVFIKAGFPKGVFQNLILHHDKTDMILKHDAVQAVSLTGSERAGSSVAQIS
                     GKYIKRSLLELGGSNSFIVWGDADIDAAVKIALTARMLNCGQSCIAAKRFILVENIYD
                     EFVSKFTDAISKLISGDPLNEETQVGPLARKDLADDLHEQIVKSIEQGAELLLGGNQV
                     DTYHEPTVLGNVKPGMTAFEEETFGPLAAMIKAKDEEDAFRLSELSRFGLGVTVCTCN
                     IEKAISMAGRVSDGAYFVNELVKSDPRLPFGGTKISGYGRELAKDGMMEFVNRKTVYV
                     K"
     gene            complement(11467..12195)
                     /locus_tag="PLX80_08930"
     CDS             complement(11467..12195)
                     /locus_tag="PLX80_08930"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQY20950.1"
                     /translation="MKNLRLLFSILTLSFFLISSQNVFSQTVYGDGNVVKETRTLSSF
                     KSIELNGVMNVYLKQGSNESAVIEADKNIIPYIITKVNSDRELEISSVENVSIKKSTK
                     LNVYITLKDINKLENNGVGNLSSESKLDLNDLGIENNGVGNIDLDVNCNKLELENNSV
                     GNTTLSGNIDNVDIEHNGVGNIKAYDLIADNLNIESNAVGNAEVNSQREIFITLNGMG
                     NVYYKGSASVKKLEKNGFGNVKKM"
BASE COUNT         3858 a         2227 c         2061 g         4107 t
ORIGIN      
        1 taattattaa aaaaaaaaga aactcataaa ttataatgtt tccaattgaa tttaaaaatg
       61 tatcaaagtt tattagagga agatcaattc tcaaaaatat cagtttagga tttagagaaa
      121 ataagataac ggcagtgatt ggaaaaagcg gcagcggaaa atcgacttta cttaaatgca
      181 taaacggact aataaaacca tcggagggag aagtcatatt atttggtagt tcactggatt
      241 atgaaaattt aattcctgtc agaatgcaga tcggatattc tgttcaggga acaggacttt
      301 ttccgcatct gaatattttt gaaaacattt ctttgctggc aaaacttaat gacaaaagga
      361 acgaggatat cgaatcacgg acaaaactct taatggaaat gactgatctt tcattagagc
      421 atggtaggaa atttccatat caactgtccg gcggagagca gcagcgtgcc ggactctgca
      481 gggctatgat gatgaatcca aaaatatttc ttttagatga agcattcgga gcgctggatg
      541 tcactactaa aaatgaagtt catctggaat tactgaaaat tcaaaaaaca gaaccacgca
      601 ctatagtatt agtgacacat gatctgaatg aagctttcaa gctagctgat gaaatagtaa
      661 taattgaaaa aggtgaaatt cagcagaaag gaaacagaga tgaaatttta aattcacctg
      721 caaatgattt tgtaaaatat tttacagcat cgcagttaat atgaaaattc tttttacaat
      781 aatattaatt ctgttttcat tttcaaaatt aaattctcaa acatctcaga caataaaggt
      841 tggtgcaaaa catttcaatg aaggatatat actaagtgaa atactaagtc agcttcttga
      901 aaagaacgga tttgaagtag aaagaaaatt caatctcgga ggtactttga tctgctatga
      961 agccttatcg aacggtgaca tagatctgta tccggaatac acaggaacaa tatctgaagc
     1021 cattttgaaa atgaatgaga aggtcagttt tactgaactt aaaaaaatac ttaatgaaaa
     1081 agatcttgat atatcaggtg aatatggatt taataataca tatgcactgg ctgtaaaaaa
     1141 tgaaactgcg aaaacacttt cacttgaaaa tatttccggt ctgaaagttc atcctgaact
     1201 gaaacttggt ctaagctatg aatttctgaa aagggaagac ggatggatgg agctttctga
     1261 agtttaccaa ctgaaacaaa agccggtggg actggaacac ggacttgctt ataaggcatt
     1321 agaagacggt cagataatgc tgactgatgt gtattcgaca gatggtgaaa ttcctaagta
     1381 cgatctgaag atattgaatg atgacaaaaa tttttttcca aaatactatg cagttacttt
     1441 ttataaaaat aaaattgcag acagtattaa aaatattttg aatgtactta caggagtgat
     1501 ctcggaaaaa gaaatgcagg agatgaattc aaaagtatta tttgagaaca gaacattttc
     1561 tgaagtagca ggggagttta taaaaagtaa agatcttttt aatgacaaac cagatctgaa
     1621 tactcatgat aaagaaagta tcttaagtga aatatttgca aagctcattc agcatttaaa
     1681 gctgacattt cttgcattac tcatttcaat agtgatagca gtacctgcag gaatttttat
     1741 ctacacatat tcttcatttt caaaaccggt attatatctt gcgggcttac ttcagacaat
     1801 accttcgatt gcactacttg cattcatgat cccgcttttt ggaattggat ttctgccggc
     1861 agttatcgca ttatttttat atggcttatt gccgatactc agaaatacaa ctatagcttt
     1921 attctcagtt gatccattgt taaaagaagt agctgaagga atgggtcttt caaaaaagca
     1981 aaaacttttt catatagaat tgccgttatc tttgccgacg atactcgccg gtatcagaac
     2041 tgctgcagtg attaatatag gaacggcaac attagctgca ttcattggag ccgggggact
     2101 tggtgaattt attgtgacag gacttgcatt gaacaataca aatcttattc tgatgggagc
     2161 tgtacctgcg gcattgctgg ctgtactgac agaaatattt tttgagattt tagaaaaata
     2221 tattactcct gaatatttaa agagaacaga aactattaat aatctttgat aactttcctg
     2281 atctcagcac tttttcaaaa attgagtaat tatatcatca aactcccata aacactgaca
     2341 atcagtttgt tgattcggtg tcccatttct tttttataaa ggaagggaaa attttacatc
     2401 catccaaaac gatttaaata cagaattcaa gattcatttt tttctgctaa ctttttttta
     2461 acgaccctcg ataagaacac tcgaggacag gctcaaaaag taacaaaaat gtcgcccgcg
     2521 taaaacaggc ggatactgtt aatgatcttt gataactttt ttttcagaag ttctcagatc
     2581 atttatgaat tcttcaggca ctggatatcc ttctacagaa atcagatatg aaacatgcag
     2641 atgagtctta ccttctttga gaattgtctt tctaccggtc cttccgactt caccgatctt
     2701 atcaccggga gccaggatat cgccgggttt tacactgact acagaaaggt gtgaataata
     2761 aaataatcct ttatttgtaa catcaaaaat tttcacatac ctgccgcccc taagcacaga
     2821 accaacatcc caggtcgtat ctgtagcaac aacaactcca cctgacatac tcaccacgtc
     2881 gaccggtttc aaagtcgagt catctaaaag atctttattt tcatcgagta tcataatgtc
     2941 atgagccgga tgccctttgg aattactgcc ctgaaaatag tcataagtac ttaatctgaa
     3001 atcatttcca ccttctctgt aataaataga ggtgaaattc tttaaaggga aaacccagtc
     3061 acttctttta taaactttgc cgccgttatt tttataatat tttattacgg caggttcata
     3121 gctgataaga agttcttgtg catcttcgac atcaattttc ccggttttga ttttttcatc
     3181 aagagcattc catttttcac agacgttgtt tgaagaattt gtttgtgcag aaaggtttgt
     3241 aataaatatt gaaaaaaaaa cagtaattag tatatatatt ttgaagaaat tcattggcaa
     3301 ttaaataacc caccctaacc cacccttttg aaacgggagg gaatgagagg ttatttttaa
     3361 attcgattta tttttaaaat tagagtaata ttaattaaaa tttatttcta tttactttag
     3421 tttgcttttt gatctactac ccgccagaca taatattgag tgcgctgccg ttttggaacc
     3481 acttgatctg ctggtcattc atagtttggt ttagcatgac attttcctca gtaccgtcgg
     3541 aatgtttcaa tgtcattttg atctgtatac cgggcttaat atctgataca tccaaatcta
     3601 ttttatcatc ttccctgatt ttatcatagt cagccgggtc attaaaagtg agcggaagca
     3661 ttccttgttt tttaagattg gtctcatgaa tacgcgcgaa tgattttaca atgatcgctt
     3721 taccgccaag gaatctcggc tccattgccg catgttcacg ggaagaacct tcaccgtaat
     3781 tttcttcacc aatcactacc catccgatat tttttccttt ataatgacgt gcaactctcg
     3841 gcacctcttc gtaatttccg ctgagatgat ttttgacttc atttacttta tcattaaaac
     3901 tatttaccgc accgatgaac atgttattag aaatgttatc caaatgacct ctgtatctga
     3961 gccaaggacc tgccattgaa atatgatcgg ttgtacattt accttttgct ttaagtaata
     4021 cttgtaaatt cttaaaatca ttttcaaggt caggcatttt gaaaggttca agagcttcga
     4081 gtctctggct ttcaggatga attacgactt cgacatccga acctacttcc gcaggtgcta
     4141 cataaccctc ctcacctttt aaaaatcctc tgtcaggcaa ttcatttcct gtcggaatat
     4201 cgagtaagac ttcttctcca ttttcatttc taatagtatc tgttagggga ttaaatgtta
     4261 aatttccggc aagagcaaaa gcagttacaa tttcaggcga agctacaaaa gaatgtgttg
     4321 ctgaattacc gtcatttctt ttagaaaaat ttctgttata tgaagttatg atagaatttt
     4381 tttcaccttc ctttacatca tgtcttttcc actgaccgat acaaggcccg caggcatttg
     4441 caagaacaag accaccgact ttttcaaaag cttcgagttg accgtcacgt tcgatagttg
     4501 ctctgatctg ctcagatcca ggtgtaattg taaactcaga tctggctttg atattttttg
     4561 cagttgcctg tcttgcaact gaagctgatc tttccatatc ttcataagaa gaatttgtac
     4621 aacttccgat tagggcaact ttcaactctt caggatatcc attttcaatg acagcatctt
     4681 taaactttga aattggccat gcaaggtctg gtgtgtatgg tccgttcaca tgaggctcaa
     4741 gtgtagagag atcaatttca ataatctgat cataataaga ttcagatcct tcatcagctt
     4801 taagttctgc agaaattcca tatgcaagat ttccaatttc ttcacgttca gttgctttta
     4861 aatatgcaaa cattttttca tcataaggaa agagggaagt agtcgcgcca atctctgcac
     4921 ccatattaca gatcgtgcct ttaccggtac aggaaattga tgaagtgcct tcaccgaaat
     4981 attccacgat agcaccggtg ccgcctttaa cagtaagaat tccggcaagc ttaagaatta
     5041 catcttttgg tgaagtccat ccgctgagct ttccggtaag tttgacaccg atgatttttg
     5101 gaaattttaa ttcccacggc atacctgcca tcacatctac tgcatcagcg ccgccgacac
     5161 ctatagcgat cattcccagt ccgcctgcat ttggtgtgtg tgaatcagtt ccgatcaaca
     5221 tgccgcccgg gaatgcataa ttttcgagga cgacctgatg aataatccca gcacccggct
     5281 tccagaatcc gatcccgtat ttattggaaa cacttgaaag aaaatcatag acttctttat
     5341 tgtcaccata agctcgcagc agatcattct ttgcgccgac ttcggcttcg attaaatgat
     5401 cgcagtgaac agtcgatgga actgcaactt tcggcattcc tgctgacata aactgcagca
     5461 gcgccatctg agccgttgca tcctgcatgg cgactctgtc cggtttaaaa tctgtaaagt
     5521 caattcctct gttataaggt gtcgtcggtt tttcccagag atgggcataa agaatcttct
     5581 cagtgtaggt cattggtctg ccaagtaatt tttttgcgtc ctctacttta cttttaaaat
     5641 ctgcgtaaac gtttttgatt aattcgagat caaacatttt tatatcctgt tataagttta
     5701 ttaaaaattt attgtatttt ttgattcttt gaaatatttt tttatagata ctaatattcc
     5761 tgaaagcata atttatagaa gccaaaatat catccttttg taatatggtt ataagatttg
     5821 aaaatctgta tttaatgaat tattcttgtg tttcagtttc tgcattttca ttttcattat
     5881 ctgcatcctc atctgcaaat attatatcat catctttcaa aagctccagc cctttaaact
     5941 tcagaagtat ctgtgcaact gtcacaatat ctttctgaca atatttcaca atcctcatca
     6001 gatcattttc ttcccagtaa attcgccaga catcgctgcc gtcaatgtcg tcttttggtg
     6061 atttgattcc aagtgcacca gcaaggagaa tcaatgaagt ataactttta taatcaccaa
     6121 acttccacat atccattgta tcaagatttt ttatttccca tggtttcttt cctgaattat
     6181 ccagtatagc aggtatcttc aatccgttaa ttatgcacct tcttgcaata taaggataat
     6241 caaattcttt accattgtga gcgcataaat aattatcatc agagttcaga ttattgttaa
     6301 gcatttcaga aaattcctca agtaaaattt tttcatcatc tccataaaaa gattttattc
     6361 taaaaatccc gtcttttgta aatactccgc agcttataca gattattttt ccaaactcag
     6421 cataaatacc tgcctgatca tatacatcac cgccggtttt atcttcagga attttaaact
     6481 tcattttctt ttcccataaa gctttccagc tgtcttccag ttcgctataa ctttttctct
     6541 gaggtactgt ctccacatcc aaaaccatta ctttagtcag atcaatttga tcaagcataa
     6601 tttcctcctt atattttaat aaatactttt aacatactcc atcagagtct ttgattattt
     6661 ttgtaataac atcttccggt aatttatcaa tacttgtaaa tctgatacag ctcttaccgc
     6721 agctaactcc gtttaaaact tttaaaagat ctctattctt ctcaacagat ttactgtcag
     6781 catagaaaga catgtaattc ttctgagaat taaatgccag tatcacttta tcatttacat
     6841 catatgtagg cattccgtac ttcatacttt cttttatacc cgggacagtt tttttaatta
     6901 tctttcttaa aattgtaaga tccttttttc tgtcagcatc tatcttctcc ataaattcac
     6961 ttactgtttc ggcttttgtg atcataacaa ttatagaata aaattactta ttttgttaac
     7021 ttttctttta aaacttcagg atcatttacc ggcagttcac atttataatt ttcacaaaca
     7081 aatacttctg ctttattact ttcattcttt ttacctgtaa tgttttcaat aaaaggtgaa
     7141 atctttccca actcttctgc agcatgcagc tttacactgt ttggaatata ttctttgcag
     7201 atcacttcat tgagtttttt aaattcatct gtgtccttat ttccgctgat aataatttct
     7261 ttcggagtat tcagtaaaaa gcttaagtca caaagcatct gcggacttga gaatgggagt
     7321 cttctgagat cttcagaaaa aatattcaga catctgtcgg ctttgtctga caggtcttga
     7381 tcatcggtca tgtatccgag cttaagtaag tttccgacct gtatagaatt acctgaaggt
     7441 tctgcaccgt cataagtatc tttggttttt aacagaatat ccttttcatt tctttccaca
     7501 tcaaaaaacc ctgcggagct gttatcataa aatttttcaa tgacaagttt atttagctca
     7561 atcgcaagag ttagatgttt tatttcataa ccggcttcgt acatattgat cagacctgaa
     7621 ataaaaaatg catagtcttc aagcgtccca ttaaatctcg actcaccatc tctgtatctg
     7681 tgaagtaaaa ctttatcctc aggtttgtat agatttttca aaatgaaatc cactgccgct
     7741 aaagctctgt cataatattt ttccttgcct gttgcctggt ataattttgc gaaagctgaa
     7801 attgtaagtc cgttccatga agtaaggatc ttgtcatcca gataaggtct ggatctttct
     7861 gatctttcag caagcagttc agttctgcag tcatcgatga ttctagcaat ttcttcaggc
     7921 attttgtcaa aatttttacc tgtttcatat atatcatttg caatatacag cacattcttt
     7981 ttgccaaaga cctcgtgcgg atcgttgaca gtattaccgt ttgtcagaat gccgtagtaa
     8041 taattaaaaa tttcagcatt atctttgcca agaatttttt caatctcttc cttttcccag
     8101 agattaaatg caccttcttc tttgacatca ggcttatttt tatccggtgc actttcagca
     8161 tcttcagcag aataaaatcc tccgttttca tcagaaagaa tatttaaaac atattccgca
     8221 gtatcttcgg ctacatataa ataaaattta ttttttgtaa caagataagt atctgtgtaa
     8281 gcagatataa gctgagcctg atcataaagc atcttttcaa aatgcggcac tcgccagaaa
     8341 ttgtccaccg agtatctgtg aaatcctcct tccagatgat catacattcc tccttcgtac
     8401 atttttttca aagaaaaagt aaccgcgtcg agagctaaaa agtctttatt gtgataataa
     8461 tgatcgagta aaaaattata tactaccggc cttggaaatt tatttcccga gccaaaaccg
     8521 ccttcgatct catcatagat cgacatggta tttttaaatg cattttcaag aatttccttt
     8581 gacaatgtct tatcaccgtc actgctgagt ctggtattta taagctcctg taatttcaaa
     8641 actatattat caccgctatt cattacatcg gatcttttat tttcccatgc atcatttatt
     8701 tgtgtgatga catcttcaaa acccgctctg ccatattttg cttttggtgg aatgtaagtc
     8761 gcaccgtaaa aaggtttgag ttcaggagta agaaacatac tcatcggcca tccgcctgag
     8821 ccggtcaaag cctgcagcgc agtcatataa actctgtcca catcaggtct ttcttctctg
     8881 tccactttta tattgatgaa aaattcatta atcagtttag cgatctcttc attttcaaaa
     8941 acctcacgct ccatgacatg gcaccagtag caggtagaat atcctattga taaaaatatc
     9001 ggtttatctt ctttccggga aatttcaaaa gtgtcttcag accatggatg ccaatcgact
     9061 ggattatacg catgttgtaa aaggtaagga cttttttcgt taagtaattt attggatttg
     9121 agtttacttt gcattaagtt cggattttag atattagaag aacaagtttt aatttaagga
     9181 atttaaagaa atgaataaat gtaataagaa tgatattttg aaaaaatttg ctttttattc
     9241 acattctccc aaatagtttt atataacaat aatgattcat tgttttctac ttactttttt
     9301 gaacgaccaa aaaaagtaac aaaaaaaatc gtcccgagtt atttttgaat ttgtaaaatg
     9361 ttttggacac taatgctttt ttatcatacc atatattaaa aatagatttt gcatttaata
     9421 tttaaatgtt tatatttttc taacaaaggg atcagactgg ataggagaaa ttttgtagtt
     9481 tcgtagtgcc cggtaaaaaa acttttaaag ttttatttga caaaagagaa ttgaaatcaa
     9541 ggacatgaaa actttttaat ctaaagtaaa aattaaaatg gaatttaaat ctgtaaatcc
     9601 ttacagcgga aaagaagccg gcattcacaa agagcataca gaagaagagt tatctgaaat
     9661 gctgaataaa gcaggcgaat catttaacag ctggagaaac gtcccgatag ttgaacggtc
     9721 cgaattaatg agaaatgcag gtcaggtact tcgtaataat actgaggagt atgcaaggat
     9781 gatcacactt gaaatgggaa aaccaattac tgaatcccga gcagagataa ataagtgtgc
     9841 ttgggtctgt gattattatg cggataatgc ggaaggattt ctttcagatg agatcattgt
     9901 aacagatgca cagaaaagtt ttgtaagaca tgatccaatt ggctgtgtgc ttgctgttat
     9961 gccgtggaat tttcctttct ggcaggtctt cagatttgct gcgcctacac tcacagcagg
    10021 gaatacagca atgctgaaac attcttcaaa tgtattcggc tgtgcgaagc agatagaaga
    10081 tgtatttatt aaagcgggat ttccgaaagg tgtttttcag aatttaattt tacaccatga
    10141 taaaacagat atgatactga agcatgatgc tgttcaggca gtatctctta ccggcagtga
    10201 gagagcggga tcatcggtgg cgcagatctc aggcaagtat ataaaaagat ctttacttga
    10261 gcttggcgga agtaattcat ttattgtgtg gggagatgca gatatcgatg cagcagtaaa
    10321 gattgcactg acagcaagaa tgctgaactg cggacaaagc tgtattgctg caaaaaggtt
    10381 tatacttgta gaaaatattt atgatgaatt tgtaagtaag tttactgatg caataagtaa
    10441 actcatatcc ggggatccat taaatgaaga aacacaggtc ggacctttag caaggaaaga
    10501 tcttgcagat gatcttcatg aacagattgt aaaatcaatt gagcaaggtg cagaattact
    10561 tttaggcgga aatcaggtag atacatatca cgagccgact gttttaggaa atgtaaagcc
    10621 gggcatgaca gcttttgaag aagagacatt tggtccactt gcagcaatga taaaagctaa
    10681 agatgaggaa gatgcattca gactttctga attatcaaga tttggattag gtgttacagt
    10741 ttgtacttgt aatattgaga aagcaatttc catggcgggc agggttagtg acggggcata
    10801 ttttgtaaat gaacttgtaa aatccgatcc tagattacca tttggtggaa caaaaatttc
    10861 gggctatgga agagagcttg caaaggatgg tatgatggag tttgtgaaca ggaagacggt
    10921 gtatgttaag tgaattttaa attttaaata attttttcat tatatgcaga gtgcgaagaa
    10981 acgtaactct gactgtaaaa aatttttata tttttagaag aataaattga agggatgaat
    11041 ttgaaaggca gtttaatttc tagtgagtaa ctaagcggag ttaagtttag agcggaacag
    11101 ttttattgaa gattattttt cctgaccatt cccataaata atctttaaat tagtttatta
    11161 ttttgtatgc aaacataact tttgagaatt gtctgaaaga tctggtatgc tcgattagtg
    11221 tccggatatt aattaaaatt ttatatataa atcagattag atcttttagt attcgattct
    11281 ttttctgtca gattccggtt ataaattcta gatctattgc aggtgacgaa accagtcagg
    11341 ttttcgcagt catcaaactt caaatcccat tcccaaattc aaaacctgac aggttttcac
    11401 acaaacacat taataaaaca attaaatcaa aaaactaaat acatatatat attccaacaa
    11461 acttcactac attttcttca catttccaaa accattcttc tccagcttct ttacacttgc
    11521 acttccttta taatacacat ttcccattcc attcaaagta ataaatattt ctctctgtga
    11581 attgacctct gcattgccaa ctgcattact ctcaatattc agattatccg caatcagatc
    11641 atatgcttta atatttccaa ctccgttatg ttcaatatca acattgtcga tatttcctga
    11701 aagagtagta ttgcctacag aattattttc aagttcaagt ttattacagt tcacatccag
    11761 atcgatattt ccaactccgt tattttctat tccaagatca tttagatcga gttttgattc
    11821 tgaactcaaa tttccgactc cattgttttc aagcttatta atgtctttta aagtaatgta
    11881 aacattcagc ttagtagact ttttaatgct gacattttcg acagaactga tctcgagttc
    11941 acggtcgctg ttaacttttg tgataatgta tggaattata tttttatctg cttcaataac
    12001 tgcagattcg tttgaacctt gtttcagata tacattcatt acaccattta actcaataga
    12061 tttaaacgac gaaagtgttc ttgtttcttt tactacattt ccgtcaccat atacagtctg
    12121 agaaaatacg ttttgagatg aaattaaaaa aaaggaaagt gtaagaattg aaaaaagtaa
    12181 tctaagattt ttcatgataa attaatttta gttaaagttt aagaaaagtt ttttagacta
    12241 acgaagtgag att
//
LOCUS       DAORVQ010000097        12135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2534_length_12135_cov_5.230546, whole genome shotgun sequence.
ACCESSION   DAORVQ010000097 DAORVQ010000000
VERSION     DAORVQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_12135_cov_5.230546"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..692)
                     /locus_tag="PLX80_08935"
     CDS             complement(180..692)
                     /locus_tag="PLX80_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQY20951.1"
                     /translation="MIIPFNNIIPEIHNSVFIADNATVIGDVIICKDASIWFNSVLRG
                     DVKRIFIDERTNIQDGCILHTSGGRSEVIIGKYVTVGHNAIVHGCKIFDNVLIGMGSI
                     ILDDAVINSNSIIAAGSVVTERCVIPEGVMVAGVPAKIIREVTDKEIYNITQSALHYT
                     ENAQKYIKSH"
     gene            complement(741..1277)
                     /locus_tag="PLX80_08940"
     CDS             complement(741..1277)
                     /locus_tag="PLX80_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HQY20952.1"
                     /translation="MSPRQRRLISDFRKMKEEFSNHPFISFEYDSEMLPPDRYVVTYR
                     HVKGLKLAEASAERKELEIITEHQIEIYLHIDYPRLKPQSYIMTKIFHPNFRMASPND
                     ICIGDYWASGETLVDIIYQIGEMITYKTYNVTSPLNGIAAKWAKENSEVFPIDNVNLR
                     QGEVEIHFSEEKDQIDIN"
     gene            complement(1302..2465)
                     /locus_tag="PLX80_08945"
     CDS             complement(1302..2465)
                     /locus_tag="PLX80_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQY20953.1"
                     /translation="MNIDLQDSPGNHVFKKLEFPDNHYFLGSGEPDIEYRIFIKQETL
                     KKIEDHLLSDVNNELGGVMVGEICINENDKKFILINDLIIAMHSSSSLSRLTFTHETW
                     DYINDVMDKDFPGKKILGWYHSHPGHTVFLSNYDLFIHENFFNLEHMVAYVFDPTIKE
                     RGFFVWENGKIEKSEGFFVFDYDEEEFNELIKLKPSDDLITIKEGKAEQEKRKYKIEL
                     NNKALVILNLLIFIILLLMIYNFYEINKLSELRDEHLRDLSFIKEDTKNLNERLNNYI
                     LETELRTKGSIITEKPKIEPVQEKITADTSAKKTGTVGNTENKVKEEKIAVTPEMVST
                     KKYTVKNGDTLEKIAMNQYKNRAGIEVIMKVNKLKDRADIKIGQVLEIPEKLE"
     gene            complement(2528..3592)
                     /locus_tag="PLX80_08950"
     CDS             complement(2528..3592)
                     /locus_tag="PLX80_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQY20954.1"
                     /translation="MKKLSGLLILILFTIVFNSCSRDEINTPPTVVLLNSKGAYVISE
                     GGFNPGTSKLSFYNKTYSDFSSSNFNPGNLGLLPDGILYENGNLYISEQGNFGATGKI
                     YRTDTTGTVQMQGPAGINPYSLAIANNKIYLTNGPSNSVTVLDKNTFSNIATIGVGIY
                     PQEILAIGNRVFVCNTSVFNGNTDSTVSVIDALTDNIIATIPVSKTPSSLAEAPDGSL
                     LVGCPGNSTGAVIYKIDPTALIIVNSYRDHTHGLCKDIVTSGSNEILYIGGDIYAEQS
                     IVKYNLSNRTAVSIIPKPVSGLNYGLNYDSEENHIYVCVAASDFTSNGKFRVYGYNGT
                     LISDFDISGGISPRRIALKK"
     gene            complement(3610..3822)
                     /locus_tag="PLX80_08955"
     CDS             complement(3610..3822)
                     /locus_tag="PLX80_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY20955.1"
                     /translation="MSIYKDKYPVKKCVCFNVTFLEMKNIMKEKGIDTIEELKEIKEL
                     AGNCKICVPYIKKMIQTGKTEFEIIK"
     gene            complement(3826..4479)
                     /locus_tag="PLX80_08960"
     CDS             complement(3826..4479)
                     /locus_tag="PLX80_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQY20956.1"
                     /translation="MKIDDAKKAGGKKYKEKILETIEGYKNLSEEDKKCFHIYYGYGK
                     GKTTCVMGLILRALGAGLKVRLVQFDKGYEGMNEHYSELNTLRKLKETGYDVVFFSTG
                     CERMNDDGTFRFKNSDDDFKEAQRALKISRELITKGDQDLLILDEAIAAVVYHLIDKN
                     EIEEIIKLWNENKRFELVMTGHKLWDGLEEKADLITEMRKVKHYFDKGIPIRKGIDF"
     gene            complement(4498..6429)
                     /locus_tag="PLX80_08965"
     CDS             complement(4498..6429)
                     /locus_tag="PLX80_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY20957.1"
                     /translation="MVKIFVNLVIVLTIFSASAELSAQLTSVAEDSSVYKTEEIKVIS
                     NKLISNELHAYSKIQFINEAQIKNSNGDRLSDILSSSNGVFIKSYGGNSSLSTISISG
                     SGSEHTLILLNGVKLNSSQNNLVDLSTITKDNIKSIEILHSGSGTVYGSEAMAGVVNI
                     ITKQNISDKIGLTLNAQSGSYGLMKYFAGITKNFSGIETGFNYSNESSENDFDYYYYN
                     GVSNVLKFRQHSAYEFSNYNFNLNFMNIENSELRVYSNYSNNIRDIPGIEVGSDPSDA
                     EQSDKNWNNILSFSTKLKSDLNLTTIFNFQNNLSNYSDNILTDSYYKNLVYTGSLQAY
                     LLRENYKLTGGYEYSFNSLISNEVDTDANRDINSVYLLSEVEVNDMLRIYPSVRADNY
                     SDPGSAVFTGQFGLNVKPFSGENIYFKANAGNNYSAPTFNELYWKDLGNRDLNPERSF
                     NAGGGVIYKFDLYSENKIELTYNYINYDDKIVWTPQSNGNWVPENRTESISNIFLADM
                     NLHKDIGNFFTADMNLNYSFTNAENINTLNPDFNNKQLIYVPLNIVNVSLELKYKNSG
                     VNVFYNFTDKRYTDPYNDKYLPAFVTVDGNVFYMLDIYGVNSTIRMEVNNIFNENYQV
                     VSGYPMPLRNLKFSVNLEY"
     regulatory      complement(6540..6730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(6771..7172)
                     /locus_tag="PLX80_08970"
     CDS             complement(6771..7172)
                     /locus_tag="PLX80_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20958.1"
                     /translation="MKIIFLFLAIVLYSCGDKGENSSSDNSTNQSETAEDTTMTPDEV
                     FSTSLIQDIIGVEEDIDLQVYLEEEIYPMLPTAGKVTIDKISSSLYLFSYQESGASKN
                     FILKKYFDPQNNEIKFEKTETQLTPEDQFLK"
     gene            complement(7177..8370)
                     /locus_tag="PLX80_08975"
     CDS             complement(7177..8370)
                     /locus_tag="PLX80_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HQY20959.1"
                     /translation="MDLFDKCNKFTRADEVKAAGFYPYFRAIEANEGPVVKIEGRDII
                     MAGSNNYLGLTAHPKVKEAALKAIEDYGTGCSGSRYLTGTLDLHIKMEEKLADFLGKE
                     SVLLFSTGYQTAQGIIPTLVTKGEYTISDKDNHACIVAANMMAKGAFAEFKRYKHNDM
                     EDLEKVLKLIPVETPKLVSSDGVFSTTGEIVNLPELVRLCKQYNARLLIDDAHSTGVL
                     GVGGRGTASEFGLVDEVDMVMGTFSKTFASLGGFVGGDKVVINYLKHHSPALIFSASP
                     TPASVAAADAALDILIAEPERIINLIANADKMRKGFKEMGFNVIDSRTGIVPVVLGDD
                     MLVFQFWRKLFDAGIFVNAFISPGVPPGMAMLRTSYMATHKDEQLNKILEIFGEIGKE
                     MGIIN"
     gene            8477..9460
                     /locus_tag="PLX80_08980"
     CDS             8477..9460
                     /locus_tag="PLX80_08980"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY20960.1"
                     /translation="MKAFITGATGFVGSHLADKLLEKNYDVYCLKRSTSSTKWLDGKN
                     INFIDGDLFSNETLENVIKDMDYVFHVAGVVKSRTAEGFYKSNYESTKNLLEITYKVN
                     PDLKKFVLISSLAATGPAIEQDSVDENSPPFPITTYGLSKLKGEEITLEYKDKLNVTI
                     IAPPAVFGPRDTEIFIYFQTFQKGLNSVIGFKEKYLSLVYVEDLADGIILAAESPESN
                     GEKYFLCFDKAYNWVDIGNVTSKLLGKKAIKLKLPHSVLYTVGYLSEVYSYFTKKAVT
                     LNVEKCKDITQIRWVCSNEKAKSKLGFKPKYTLEESFKKTIDWYKEMKWIK"
     gene            9467..11122
                     /gene="argS"
                     /locus_tag="PLX80_08985"
     CDS             9467..11122
                     /gene="argS"
                     /locus_tag="PLX80_08985"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQY20961.1"
                     /translation="MSKIKDQVSEILQSAIKECGFPEREIVLDIPKDEKFGDASSNVA
                     MTLSKELKTPPRKIAEEIISKISIDNKLITKTEIAGAGFINFFLSDDLYKNELKNILG
                     DGNDYGKSELHKGQTANLEWVSANPTGPLHTGHGRQLCLGKAIAILLEWTGYKVTREY
                     YYNDAGNQMNNLAKSVRARYMQIFENEFPFPEYGYVGEYVNEIANLIYEKHGESLKDS
                     DDLDYFKKEGEVFTFDVIKKTLSNLGIHHDIFFNETSLYEKKEIEKTLEAFKEKDLSY
                     ELDGATWLKMDENEGIQKDKVIVKTTGEPTYRLPDIAYHIDKIKRGYDLIIDIFGSDH
                     GDTYKEVLYGLKSLGYNTDKIKVIIHQMVTFKMGNESVKMSKRSDNVYYLDELISDIG
                     SDAMQFFYVMRGANTHLDFDLSLAKDHSEKNPVYYLQYAHARICGILRNADEVFPDYK
                     KDLINSLNLDLINSPEEMKLIKILTEFPDEVLSAAKTYEPHKIITYLNTVAENFHRFY
                     HNNRVIDPENKEISLSRIHICLSVKQVLKNGFDIIGITAPERM"
     gene            complement(11621..>12135)
                     /locus_tag="PLX80_08990"
     CDS             complement(11621..>12135)
                     /locus_tag="PLX80_08990"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20962.1"
                     /translation="DGENTPDTETQRWASIEVDSEGQPILIAMEWNGNNAQWKARKYD
                     TDGNVIFTIGEEDNSDLNSNSKTNATSDLNRSDSDNDISKTQLLQNKPNPFNPSTEIF
                     YNVSQNSFINIKVYNLLGQEVATLVNEIKQPGKYQIRFDGSALSSGMYFYKMFVNEIA
                     TDSKRMLLLK"
BASE COUNT         3958 a         2176 c         1792 g         4209 t
ORIGIN      
        1 ttttttttta tttaaagaat ttaatcctgt ttatataaaa tcaagatttc tttattttaa
       61 tactcattaa aagaattagc atataaaaat atagatataa tataactttg tcaattgata
      121 atatctcttt attattttgt ctataaaaat tcaataaaat tttagttttg tttaaaaatt
      181 taatgagact ttatatactt ttgtgcattt tctgtataat gcaatgcgct ttgtgtaatg
      241 ttatagatct ctttgtcagt tacttccctt atgatttttg ccggaactcc tgcaaccatt
      301 accccttcag ggataacaca cctttcagta actacagatc ccgcagcaat tatagagttt
      361 gaatttataa cagcatcatc cagaataatc gaccccattc caatcaaaac attgtcaaaa
      421 attttacaac catgtacaat tgcattatga ccaactgtaa catatttccc tattattact
      481 tctgatctgc cgcctgaagt atgcaatata caaccatcct gaatatttgt tctttcatca
      541 atgaaaatcc ttttcacatc acctctcaac acagagttga accagatact ggcatcttta
      601 catataataa catctcctat cacagtagca ttatctgcaa taaaaacgct gttatgtatt
      661 tcaggaatta tattattgaa tggtatgatc aatttttaag aataggataa tataaaaaac
      721 tttgctcttc tttaataatg ttaatttatg tctatctgat ctttctcttc actaaaatga
      781 atttcgactt caccttgtct gagatttaca ttgtcaattg ggaaaacttc tgaattctct
      841 tttgcccatt tcgctgcaat gccgtttaat ggactggtaa cattataggt tttataagtg
      901 atcatctcac ctatctgata aataatgtca actaaagttt ccccggatgc ccagtaatct
      961 ccgatacaaa tatcattggg agaagccatc ctgaaattgg gatggaatat ttttgtcata
     1021 atataactct gaggctttaa ccttggataa tcaatatgta aatatatttc aatctgatgt
     1081 tcagtaataa tttcaagttc ttttctttcc gcagaagctt cagcaagttt taatcctttt
     1141 acatgtctat atgtaactac atatctgtca ggaggcagca tttcagaatc atattcaaaa
     1201 cttatgaaag gatgatttga gaattcttct ttcattttcc tgaaatctga gatcaatctt
     1261 ctttgtctgg gactcattta taatttttaa ataaaaattt tttattcaag tttttcaggt
     1321 atttcaagta cctgtccaat ctttatatct gctctgtctt ttaatttatt aactttcatt
     1381 attacttcaa tccctgcacg gtttttgtat tgattcattg caattttctc aagtgtatca
     1441 ccatttttta cagtgtattt tttagtactg accatttccg gggttacagc aattttttct
     1501 tccttcactt tattttcagt attcccgaca gtgccggtct ttttagcaga agtatccgca
     1561 gttatctttt cctgtacagg ttctattttt ggtttttcag ttattatcga tcctttggtt
     1621 cttaattctg tttctaaaat ataattattc agtctttcat taagattttt tgtatcctct
     1681 tttataaaag acaggtctcg caaatgttca tctcttaatt ctgataattt atttatttca
     1741 taaaaattat aaatcatcag aagtaatata ataaatatca gtaaatttaa tatgaccagt
     1801 gctttattat tcaattcaat tttatatttt cttttttcct gttcagcttt gccttcttta
     1861 attgtgatca gatcatcaga aggttttaac ttaataagtt cattaaactc ttcctcatca
     1921 taatcaaaca caaaaaaacc ttcagatttt tcaatttttc cattttccca tacaaaaaat
     1981 cctctttcct ttatagtagg atcgaagaca taagcaacca tatgctcaag gttgaaaaaa
     2041 ttttcatgaa tgaaaagatc ataattagaa agaaaaactg tatgccccgg atgtgaatga
     2101 taccatccga gaatcttctt cccaggaaaa tccttatcca taacatcatt tatataatcc
     2161 caggtctcat gcgtaaaagt gagtcttgac aacgaagagc tgctgtgcat tgctattatt
     2221 aaatcattga taagaataaa tttcttatca ttttcattaa tgcagatctc accgaccatt
     2281 acgccgccaa gctcattatt tacatcgctt aataaatgat cttcaatctt cttaagagtc
     2341 tcctgcttaa taaagattct gtattctata tctggttcac cactcccaag gaaataatgg
     2401 ttatcaggga attcaagttt tttaaaaaca tgattccccg gagaatcctg taaatcaata
     2461 ttcatacatt acttttggta ttgtgcaata taagtaatgt atgaagtatt ttaaatatta
     2521 atttagatta tttttttaaa gcaattcttc tcggacttat gccgcctgaa atgtcaaaat
     2581 cgcttatcag agtaccatta taaccataga ccctgaattt cccgtttgat gtaaaatccg
     2641 atgcagcaac acaaacatag atatgatttt cttcgctatc ataattcaga ccgtaattca
     2701 aacctgaaac tggtttaggg attatggata ctgccgttct gttgctaaga ttgtatttta
     2761 ctatactttg ttctgcataa atatcacctc ctatatataa aatttcattc gagcccgaag
     2821 tgacgatatc tttgcaaaga ccatgagtat gatcacgata tgaattaact ataattaaag
     2881 cagtaggatc tatcttataa ataactgcgc cagttgagtt tcccgggcat ccaaccagta
     2941 aacttccgtc cggagcctct gcaagcgatg acggggtttt tgaaacagga atagtcgcta
     3001 ttatattatc ggtaagggcg tctattacag acacagtcga atctgtattt ccattaaaaa
     3061 cactggtgtt acaaacaaaa accctgttac caattgcaag gatctcctgt ggataaatac
     3121 cgacacctat tgttgcaata ttactgaatg tatttttatc aagaactgtc acgctgttcg
     3181 aaggtccgtt ggtcaaataa attttattat ttgcaattgc cagagaatat ggattaatgc
     3241 ctgcaggtcc ctgcatctga acagttccag tcgtatcagt cctatatatt tttccagtag
     3301 caccgaaatt tccctgctca ctgatgtata gatttccgtt ttcatataaa ataccatccg
     3361 gcaaaagacc taaatttccc ggattgaaat tacttgaact aaagtctgaa taagtcttat
     3421 tataaaaaga taatttactt gtacctggat taaaacctcc ttcggaaatt acatatgcgc
     3481 cttttgaatt aagtaaaacc accgtcggcg gtgtattgat ctcatctctt gaacaagagt
     3541 taaatacaat agtaaatagg attaggatta acaatcctga taattttttc atttttatat
     3601 cgtttaaatt tattttataa tttcaaattc tgtttttcca gtttgaatca ttttctttat
     3661 atatggaaca caaattttac aattacctgc taactctttt atttccttca attcttcaat
     3721 cgtatcaatt cctttttcct tcatgatatt tttcatttcc agaaaagtaa cattaaagca
     3781 aacacatttc tttacaggat atttatcttt ataaatactc actccttaaa aatctattcc
     3841 tttcctgatc ggaatccctt tatcaaaata atgtttaact tttctcatct cagtgatcag
     3901 atcagctttt tcctcaagac catcccataa cttatgaccc gtcataacaa gttcaaacct
     3961 tttattctca ttccataatt ttattatttc ctcaatttca tttttatcta tcaaatgata
     4021 aacaacggca gcaattgctt catcgagaat cagcaaatcc tgatcccctt tcgtaattaa
     4081 ttctcttgaa attttcagcg ctctttgagc ttccttaaaa tcatcatccg aatttttaaa
     4141 cctgaaagta ccgtcatcat tcatcctttc acatcctgtt gaaaaaaaca cgacatcata
     4201 tcctgtctct tttaatttcc ttagagtatt aagttcagaa taatgttcat tcattccttc
     4261 atatcctttg tcaaactgaa caagcctaac cttcagtccg gctccaagtg ctcttagtat
     4321 caaacccatt acacaggtcg tcttaccttt tccgtatccg taataaatat gaaaacattt
     4381 tttatcttct tcggaaagat ttttatatcc ttctatggtc tcaagtattt tttctttgta
     4441 ttttttccca ccggcttttt ttgcatcatc tattttcatt attacttttt attttattta
     4501 atattcaaga ttcacagaaa attttagatt ccttaaaggc ataggatagc ctgaaactac
     4561 ctgataattt tcattaaaaa tattatttac ttccatcctt atggtagaat ttactccata
     4621 aatatccagc atataaaaaa cattaccgtc aacagtaaca aatgcaggta aatatttatc
     4681 attatatgga tctgtatatc tcttatcagt aaaattatag aacacattta ctcctgaatt
     4741 tttatatttc aattcaagac ttacatttac aatattcaat ggtacataaa ttaactgctt
     4801 attattgaag tcaggattta aggtattaat gttttctgca tttgtaaatg aataattcaa
     4861 attcatatct gctgtaaaaa aattcccaat atccttatgt aaattcatat cagctaaaaa
     4921 aatatttgaa atcgattctg ttctgttctc aggaacccag ttgccatttg actgaggtgt
     4981 ccatacaatt ttgtcatcat aattaatgta attataagta agctcaattt tattttcaga
     5041 atataaatca aatttataaa taactccccc acctgcgtta aatgacctct caggattcag
     5101 atctctgttg ccaagatctt tccagtacaa ttcattgaat gtcggtgcag aataattatt
     5161 tcctgcattt gctttgaaat atatattctc tccggaaaaa ggttttacat ttaatccaaa
     5221 ctgacctgta aatactgccg agcccggatc cgagtaatta tctgctctca cagaaggata
     5281 tattctaagc atgtcattta cctcaacctc agataataaa tatacactgt taatatctct
     5341 gttagcatct gtatccactt cattactaat taaagaatta aaactgtatt catatcctcc
     5401 tgtaagttta taattttcac gcaacagata cgcctgtaac gaaccagtgt aaactaaatt
     5461 tttgtaatag ctgtctgtta aaatattatc gctgtaattg gaaagattat tctgaaaatt
     5521 gaaaatagtt gttagattga gatcagattt taatttagta ctaaaagata gaatgttgtt
     5581 ccagtttttg tctgactgtt cagcatcaga cggatcagaa ccgacttcaa ttccaggtat
     5641 gtctcttatg ttgtttgaat aattcgaata aactctcaat tcagaatttt caatattcat
     5701 gaaatttaga ttaaaattat aattagaaaa ttcatatgca gaatgttgtc gaaactttaa
     5761 cacattactt actccattat aataataata gtcaaagtca ttttcagaag attcatttga
     5821 ataattaaat cctgtctcaa ttcctgaaaa atttttggta atccctgcaa aatatttcat
     5881 aagtccgtaa gatccagact gagcatttaa ggttagtccg atcttatctg agatattttg
     5941 tttggtaata atattaacaa ctcctgccat tgcttccgag ccgtaaactg taccagagcc
     6001 tgagtgcaga atttctatac tttttatatt atcttttgtg atagtgctaa gatcgacaag
     6061 attgttttga gaggaattta acttaacacc attgagcagg attaaagtat gttcagagcc
     6121 tgaaccgctg attgaaatcg tacttaaaga agaattacca ccgtatgatt ttatgaacac
     6181 tccatttgaa gaactgagaa tatctgagag tctgtccccg tttgaattct ttatctgcgc
     6241 ttcattaata aactgaattt ttgaatacgc atgaagttca ttactgataa gtttattact
     6301 aattactttt atctcttcgg ttttatatac agatgaatct tctgcaaccg aagtcaactg
     6361 cgcacttagc tcagccgatg cggaaaagat cgttaaaacg atcaccagat tcacaaaaat
     6421 tttaaccaaa aaaaactcct taactcttat ttaattcatt taaaggggat ttaaataaat
     6481 gaatctcaaa agaataaagg agtctcttgc acattcatta atcttttccc acgaagatca
     6541 ttgaaatgaa ttattacggc aggtctcctg acttaaaagc aatttcctta tatccttccc
     6601 acacaataaa tgagcagtgg attcttgata aggtcttctt tattacagtt gcggggacag
     6661 taacggaatt tcaccgtatt ccctattatt cctcccttta cgggtaagaa ccataataat
     6721 tatttataag aactatacaa aaataaacaa attactttac tgaaacaaat ttattttaag
     6781 aactgatcct caggcgtaag ctgcgtctca gttttttcaa acttaatttc attattctgc
     6841 ggatcaaaat acttcttcaa tataaaattc ttacttgcac cactttcctg atatgaaaac
     6901 aaatacaatg aagatgaaat tttatctatt gtaacttttc cggctgtcgg aagcatcgga
     6961 taaatttcct cttccagata tacctgtaga tcaatatctt cttctactcc aataatatcc
     7021 tgaatcaatg aagtactgaa aacttcatca ggtgtcatag ttgtatcctc tgcagtttca
     7081 ctttgatttg ttgaattatc cgatgaagaa ttttcacctt tgtctccaca tgaatacaat
     7141 actattgcaa gaaataaaaa aatgattttc atattatcag tttattatac ccatttcttt
     7201 tccgatctct ccgaatattt ctaaaatttt atttagctgc tcatctttat gagttgccat
     7261 atatgaagtc ctgagcattg ccattcccgg aggtacacct ggagagataa atgcatttac
     7321 aaaaattcct gcatcaaaca attttctcca gaactgaaat acaagcatgt catcaccaag
     7381 tacgacaggt acaataccgg tcctgctgtc aattacatta aatcccattt ctttaaatcc
     7441 ttttctcatt ttatcagcat tggcaatgag attaataatt ctttcaggtt cggcaattaa
     7501 aatatccaaa gctgcatctg ctgctgctac ggaagcaggc gttggtgatg ctgaaaatat
     7561 taatgcgggt gaatgatgtt tgagataatt gattactact ttatctcccc ctacaaagcc
     7621 gccgagagaa gcaaaggttt tggaaaatgt acccattacc atatcgactt catctacaag
     7681 accgaattct gaagcagttc ctctgcctcc aactcctaat acaccggtag aatgcgcatc
     7741 atcaatgagt aatcttgcat tatattgttt gcataatctt acaagttcag gcagatttac
     7801 aatctcaccg gtagtagaaa atacaccgtc agatgataca agttttggag tttccacagg
     7861 gatcagtttc aatacttttt caagatcttc catgtcgttg tgcttatatc tcttgaattc
     7921 agcgaaagct cctttagcca tcatattagc tgcgacgata catgcatgat tatctttatc
     7981 ggaaattgta tattcacctt ttgtaacgag tgtcggaatt attccctgag cagtctgata
     8041 tccggttgaa aaaagtaaaa cagattcctt tccaagaaag tcagctaatt tttcttccat
     8101 tttaatatgc aggtcaagtg ttcctgtgag atatcttgaa cccgaacatc ctgttccata
     8161 atcttcaatt gcttttagtg cagcttcttt tacttttgga tgtgctgtca gaccgagata
     8221 attattagat cctgccataa taatgtcacg tccttcaatt tttacaaccg gtccttcatt
     8281 agcttcaata gctctgaaat aaggataaaa ccctgcagct tttacttcat ctgcccttgt
     8341 gaatttattg catttatcaa aaagatccat ttataatttt tattattggt atttattaaa
     8401 tacgaatttt tgaacataga aaatacgaat tataatttat aacttctatt gataaacatt
     8461 aatcattact gaaagattga aagcatttat aacaggagct acaggttttg tcgggagtca
     8521 tttagccgat aaactgctcg aaaaaaacta cgatgtttac tgcctcaaac gaagcacttc
     8581 atctaccaaa tggctagatg gaaagaatat taatttcata gacggtgatc ttttttcaaa
     8641 tgagacactt gaaaatgtaa ttaaggatat ggactatgtc tttcatgttg caggtgtagt
     8701 aaaatcaaga actgcagaag gtttttataa aagtaattat gaatccacaa agaatcttct
     8761 tgagatcact tacaaagtaa atccggatct taaaaaattt gtacttatct cttcactggc
     8821 tgcaaccggt ccggcgatcg aacaagattc tgttgatgaa aacagtccgc ctttccctat
     8881 taccacttat ggcttatcaa aacttaaagg ggaagaaata actttagaat ataaagataa
     8941 actcaatgta actatcatag ctccgcctgc agttttcggt ccgagagata ctgaaatatt
     9001 tatttacttt cagacttttc agaaaggtct aaacagcgtg attggtttta aagaaaaata
     9061 tctaagtctt gtatatgtag aagatctggc tgacggaatc atacttgctg ctgaaagtcc
     9121 tgaatcgaat ggtgaaaaat attttctttg ttttgataaa gcatataact gggtggatat
     9181 tggaaatgta acttcaaagc ttctaggaaa aaaagcaatc aaattaaaac ttccgcattc
     9241 tgtcttatat acagttggat atttgtcaga ggtttattct tattttacaa aaaaagcagt
     9301 cacacttaat gtagaaaagt gcaaagacat tactcagatc aggtgggttt gctcaaatga
     9361 aaaagcaaaa tctaagttag gatttaaacc aaagtataca ctcgaggaaa gttttaaaaa
     9421 aacaattgac tggtataaag aaatgaaatg gataaaataa tttaatatgt ctaagataaa
     9481 agatcaggtg tcagaaattt tacaatcagc aataaaagaa tgcggctttc ctgaaaggga
     9541 gatcgtactg gatattccaa aagatgaaaa gttcggtgat gcttcatcga atgttgcaat
     9601 gacactttca aaagaattaa aaactccacc ccgcaagatc gcagaagaaa ttatttcaaa
     9661 gataagtata gataataaat taattaccaa aacagaaatc gccggtgcag gcttcataaa
     9721 tttttttctg tctgatgatc tttataaaaa tgaattaaag aatattctgg gtgatggaaa
     9781 tgattacggt aaatctgaac ttcataaagg tcagaccgca aatctcgaat gggtaagcgc
     9841 aaatcctact ggtccgcttc ataccggaca tggcagacag ctttgcctag gaaaggcaat
     9901 tgcgatttta ctggaatgga caggatataa agtaacacgt gaatattatt ataatgacgc
     9961 gggtaatcag atgaacaacc ttgcaaagtc agtcagagca agatatatgc agatctttga
    10021 aaatgaattt ccttttcctg agtatggata tgtcggtgaa tatgtaaatg aaattgcaaa
    10081 cctgatatat gaaaaacatg gagagtcttt aaaagacagt gatgaccttg attattttaa
    10141 aaaggaaggt gaagtattta catttgatgt tataaaaaag acactttcga atctgggaat
    10201 tcatcatgac atttttttta atgagacatc gctttatgaa aaaaaagaaa tagaaaaaac
    10261 tctcgaagca tttaaagaga aagatctttc ttatgaactc gacggcgcga catggctgaa
    10321 gatggatgaa aatgaaggca ttcagaaaga taaggtcatt gtaaaaacta ctggtgagcc
    10381 gacttacaga ctgccggaca ttgcttatca catagataaa ataaaaagag gttacgatct
    10441 tatcattgat attttcggtt cggatcacgg agatacatac aaagaagtat tatatggact
    10501 taaatctctc ggttataaca ctgataaaat aaaagtgatc atacatcaga tggtaacttt
    10561 taaaatggga aatgaatctg taaagatgag caagagaagt gacaatgtat attatcttga
    10621 tgaacttatc agcgacattg gctctgacgc aatgcagttc ttttatgtaa tgagaggagc
    10681 aaatactcat ctcgatttcg atctctcact tgccaaagac cattctgaaa aaaatccggt
    10741 ttattatctg cagtatgcac atgcaaggat atgcggaatt ttaagaaatg ctgatgaagt
    10801 atttcctgat tataagaaag atcttattaa tagtctgaat ctcgatttga taaattcacc
    10861 tgaagaaatg aagctcatca aaattttgac agagtttcct gatgaagtat taagcgcagc
    10921 caaaacttat gaaccgcata aaataattac ctatctgaat acagtcgctg aaaatttcca
    10981 cagattctac cataataaca gggtcattga tcctgagaat aaagaaattt ccctatcaag
    11041 aattcatatc tgtctttcag tcaaacaagt tttaaaaaac ggatttgata taattggaat
    11101 tactgcacct gagaggatgt agattttgta tttttgccat tcttattttc aaagtaaatt
    11161 ttcgctgaat cacttttatg gtgattcaga caatccagat ttcgtaattg tctgaaccgc
    11221 tgatgacact gattaatctg attttgctga ttcaaatttt ttttatcact attaataatt
    11281 aaaatttaat cctaataacc ccgaaggagt gatattattt aaaacaattt attcaaatcg
    11341 gctactgccg ataattacga ataataatgc cacccctacg gtgtttgata attattatac
    11401 ccgcatttct acaaaaatat cacccctacg gggtttataa ttattattgt agtttcatat
    11461 taaactgatt tcatagattc gaaaactttc aaattcatct gttcagaaat catgttaatc
    11521 aggtaaatct gcggaatcag cggttcagat aaaaaaacac cggctgcaaa aaacacaacc
    11581 ggtgaatcat gaaaacatat tacaagaaac aatctttggt ttatttcagc aaaagcattc
    11641 tcttactatc agttgcaatt tcattcacaa acatcttgta aaaatacatc ccgctcgaaa
    11701 gtgccgagcc atcgaatctg atctgatatt tgccgggctg ttttatttca tttacaagtg
    11761 tggcgacttc ctgtccgagc agattatata cttttatatt tataaaacta ttttgtgaga
    11821 cgttataaaa tatttctgtg gatggattga atggatttgg tttgttttgg agtaattggg
    11881 ttttggaaat atcattatca gaatcggatc tatttagatc tgaagttgca tttgtttttg
    11941 aatttgaatt cagatccgaa ttatcttctt caccaattgt aaatatcaca ttcccatcgg
    12001 tatcatattt tcttgctttc cactgagcat tatttccgtt ccattccatt gcgatcagga
    12061 tcggctgtcc ttcggagtca acttcaatag aagcccagcg ctgagtttct gtatctggtg
    12121 tgttttctcc gtcat
//
LOCUS       DAORVQ010000098        11898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2624_length_11898_cov_4.148695, whole genome shotgun sequence.
ACCESSION   DAORVQ010000098 DAORVQ010000000
VERSION     DAORVQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2624_length_11898_cov_4.148695"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLX80_08995"
     CDS             complement(<1..721)
                     /locus_tag="PLX80_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY20963.1"
                     /translation="MITRIGSKLIQFFTELGEFFHMMIGVLKSLPRIFRDRKLVFEQM
                     QHVGVNSLALVAIIGIFTGAVSCWQAAYQIKGLISYSYLGSATTKAIIIELGPVLAAI
                     VLAGRVGASMAAELGTMKVTEQIDALESMAIDPIRYLAMPRIVATTIMLPILVIYASS
                     IAILGSYSVATFFLGVPSETFLGAFKKSFEIKDLFAGSIKALFFGIAISSIGCFIGFS
                     TRGGAEGVGLATIKAFVIAAAS"
     gene            complement(747..2063)
                     /locus_tag="PLX80_09000"
     CDS             complement(747..2063)
                     /locus_tag="PLX80_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQY20964.1"
                     /translation="MTEFKTIIFDLLLLLFFVLLNALFVAAEFALVKVRKTQIKSTEI
                     KENKKAKLAIKQITHLDEYLAATQFGITISSLGLGWIGKPVTERMLDPLFVLLGIVNP
                     NTINTISLTVGFIIITFLHIILGELAPKSIAIRHPKPTTLFVSFPLQVFFLIFKPAIL
                     VLNGTANYILKLVGLNPATESEKVHSEEEIRLLITEGTLSGRIDQTESQIIQKVFDFN
                     DKTAKDVMIPRNVMFAINVEEKRENIIQKVIEEGFSRIPVFKDSLDNIVGIIYSKDII
                     SAAEHKEVIVLQDIIRHAHFIPETKQIGEILKDFQKKHIHLGIVVNEHGGVEGLVTLE
                     DILEEIVGEIEDEYDYTPEKIKSQKKGIFLVNPEINIEEFNEKFETDIPDDNEDYHTL
                     SGFLQEVTGHIPEIYERIDYQGLVMTIMKKSGNKLLQVKIQKLKSY"
     gene            complement(2181..2849)
                     /locus_tag="PLX80_09005"
     CDS             complement(2181..2849)
                     /locus_tag="PLX80_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20965.1"
                     /translation="MRSFKYILLILFISGLAIGKSVSDDPKNNVKLFNATINKNEVEI
                     NFIISNPQNITKYRIESKKVGEENFKIVNDLLFSNFRKKEVSDSLNLYFYSYFDKPTE
                     NGVYFYKLSIYDVGNKLIKSDEIKIGISEVPEFVNLQNNPNPFNPNTIISYKLLVPTN
                     VKLSVYSLTGKYVDLLVDEFQSPGLYKYDFNTSKYSDLSSGIYFYKLESSYSTDIGKM
                     IFTK"
     gene            complement(2854..3345)
                     /gene="nuoI"
                     /locus_tag="PLX80_09010"
     CDS             complement(2854..3345)
                     /gene="nuoI"
                     /locus_tag="PLX80_09010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQY20966.1"
                     /translation="MSIIKKKDLTLLEQTYLPQIVKGLGLTFKQMFRPKFTRQYPEEK
                     WIPPASYRGRPVLVQEDDGSERCVACALCSRVCPALAIEVQAAETENEKERYPEKFEI
                     NMVRCIFCGFCEEVCPEEAIIMSDEYELVFSNQEEAVFGKDKLLVPKDKLKKRLDFLQ
                     EHR"
     gene            complement(3409..4146)
                     /locus_tag="PLX80_09015"
     CDS             complement(3409..4146)
                     /locus_tag="PLX80_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY20967.1"
                     /translation="MKKLLLITTIFLISSLTASSQMVFERIGGDTTYGSTGANYAYAV
                     YSNSGTTPIQVRFDRTQDILPNAQWTSSICVGLCYAPFIHVVPDTNQIFGPEPPVTIQ
                     PGELDTMDITFDAPTLGTAHIKLRMYIDSNPSQFIENDFILTVNTVGINNISSIAESY
                     SLSQNYPNPFNPSTSIEFSITRSDIVTLKVYDILGNEVSVLVNNQRYDAGKYRADFNG
                     SSLASGIYYYKLSTGNFTDTKKMMLIK"
     gene            complement(4159..4650)
                     /locus_tag="PLX80_09020"
     CDS             complement(4159..4650)
                     /locus_tag="PLX80_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY20968.1"
                     /translation="MTKENTNPLDKSKLYLKKANDLQIKGQFEEAILNYKISLDIYPT
                     PEAHTFLGWTYSFLGDYDNAIEECKKAISLDKEFGNPYNDIGSYLIQQGKHEEALTWL
                     ELALKADRYVNKHFTHLNIGRVFESKGLWFEAVDEYKKAIDISPDYLIAKQYLNKLQG
                     LLN"
     gene            4832..5569
                     /locus_tag="PLX80_09025"
     CDS             4832..5569
                     /locus_tag="PLX80_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQY20969.1"
                     /translation="MKDKVGIILGSGLSSFSDLLTESKVLYSDEESFHKMKILTGKIS
                     GREVVIFAGRRHLYEGYSKEMILEFIDIANDHGVKLLIISNAAGGLNCNFKVSDLMLI
                     TSHLNFLKKNFIVSPNQIRYDNELSEKLLKIALSKKINLRKGTYCCLSGPNYETRSEI
                     TYLKKFGIDAVGMSTIPEVLYASEKGIKTIAISCITNLLSASGIEITDHSEVVEAGRS
                     AFKNFTEIIISIIENSNSLIQKNNNVN"
     gene            5559..6338
                     /locus_tag="PLX80_09030"
     CDS             5559..6338
                     /locus_tag="PLX80_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQY20970.1"
                     /translation="MLTDTHAHLYYPEILDNIDEILNRAKDAGIEKIIVPAVDLESSK
                     KIIDLSEKYDMIYAALGVHPGDVSKNNLSVLDEIEKMLSHEKVVAVGETGLDYYWDTS
                     NIDKQKEFFEKQIELASAYDLPVLIHTRESTDDAISIIESFLLKKDHKNIKGQFHCFS
                     GNENERKRISDLKDFYISYCGNITYKNSNLSDIVKNTPVEKLLSETDSPFLPPVPFRG
                     KKNEPSYMINTLKKISELKSMELSDLYKIISENVLNLFFRK"
     gene            complement(6440..6652)
                     /locus_tag="PLX80_09035"
     CDS             complement(6440..6652)
                     /locus_tag="PLX80_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20971.1"
                     /translation="MIQAGIAAKRPHIIYESIPSTRANIALELAPPPDPDRGAEEDDG
                     GGGGGGGGLNEGGGGGGGGTFPLPES"
     gene            6615..6830
                     /locus_tag="PLX80_09040"
     CDS             6615..6830
                     /locus_tag="PLX80_09040"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20972.1"
                     /translation="MCGLFAAIPAWIMGKKEINEINAGRSPEAGKTFATIGMWLGIIN
                     VILTVLTLIAVVFLFTLGILSSSDFDI"
     gene            6875..7333
                     /gene="dut"
                     /locus_tag="PLX80_09045"
     CDS             6875..7333
                     /gene="dut"
                     /locus_tag="PLX80_09045"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQY20973.1"
                     /translation="MKIFFKIADSGSPVFIPEYKTSGSAGMDLCSSSEETILITSSEY
                     ALVPTNLIIEIPEGFEGQIRPRSGLALNNGITVLNSPGTIDCDYRGEIKVLLINHGKK
                     DFAVKSGDRIAQLIIAKYIKCELIQSEEISDSVRGEGGYGSTGTSHNSEI"
     gene            7343..8020
                     /gene="rsmI"
                     /locus_tag="PLX80_09050"
     CDS             7343..8020
                     /gene="rsmI"
                     /locus_tag="PLX80_09050"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQY20974.1"
                     /translation="MTGILNLVTTPIGDYSDMTLRAIRSLEESDKIYCEEFKEGTKLL
                     RFFNIKKELSNLNEHNETESSEELFMELLQGKNISIITDCGTPLFSDPGKVILNKCID
                     AGIKVEFLGGANSLLSAVVLSGFDISRFFYYGFLSPKAEIRKQQLKEFIHSERVSVLL
                     DAPYRLKAVLSDISDVFEERKVFVAFNVTQESEKKFRGSAAEILNEIGEENLKGEFVI
                     IIDKYND"
     gene            8013..9002
                     /gene="corA"
                     /locus_tag="PLX80_09055"
     CDS             8013..9002
                     /gene="corA"
                     /locus_tag="PLX80_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HQY20975.1"
                     /translation="MIKCLVYNNEKGVTSVPQDKIKKWDFECSDVLWMDIYNSSTEDH
                     SQNILSDVFEFHPLAIEDSFKYYNDSKVHHPKTDDFEKYLFIVFNGIQSTGSNKRYSI
                     FSLSCFLGKNFIVTIHNESGENSISKDLKNFINEKSFKKGPDYILNLILDDIVDRYYP
                     VLDHIESEIELTENKIFKEMPSNRNLMSILNVKKELIKLRRISSYQKEVLYKLSKGDS
                     ELISMEESFYYRNVYDHLVRVSDTTESYRDYVSGILDSYLSIVNNKMNEVMKFLTIIA
                     TIMLPLTLITGIFGMNFDSIPFLHNETGFYISLTFMFIVAAIMLMWFKYKKWI"
     gene            complement(9014..9919)
                     /locus_tag="PLX80_09060"
     CDS             complement(9014..9919)
                     /locus_tag="PLX80_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQY20976.1"
                     /translation="MLQKFDERNSNIKVFIKDRLYDRADAKVSVFDSSVQGGDAVWEG
                     LRIYDGKIFCFDKHVNRLHESAKTLDFKNIPDRDFIKKAVMETLKANGMKDGAHIRLT
                     LTRGEKITSGMDPRLNTHGPCLIVLAEWKKPVYDNSEGIKLVTSSIRRNSPQNLDSKI
                     HHNNLINNILAKIEANMCGADDALMLDVNGYVSETNATNIFMVKNGTLYTPNADSCLP
                     GITRGLVMEIASKLKIDCFERNISVSEFYNADEVFTTGTMGELTPVTEIDGRKTENRS
                     DSNVLAEILSAYKLKTETEGETLDF"
     gene            complement(9924..10640)
                     /locus_tag="PLX80_09065"
     CDS             complement(9924..10640)
                     /locus_tag="PLX80_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HQY20977.1"
                     /translation="MKRICLWSSPRNISTALMYSFAQRSDTKVFDEPLYGHYLRITDS
                     DHPGKDEIIKSMKADGEKVISDIVDGKYDKEVLFFKQMTHHLIELNEDFLKDVINVFL
                     IRDPKELIISFAKVIPEVTMERIGVKKQYDLFQKLFSDGQESIVIDSGEILKDPEKVL
                     RELCEKLGIPFQKNMLRWNAGPIAEDGVWAKYWYENVHKSTGFSRPFRKDEELPEKFI
                     LLYIECKKYYNYLKDFSIKA"
     gene            10944..>11898
                     /gene="purM"
                     /locus_tag="PLX80_09070"
     CDS             10944..>11898
                     /gene="purM"
                     /locus_tag="PLX80_09070"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQY20978.1"
                     /translation="MKVSYKSSGVNISEADKFVENIKPLVKETFTKNVLKGIGNFGAF
                     YEIDIKKHNHPVFVSSVDGVGTKLKIASALKKFDTIGEDLVNHCVNDIAVCGAVPLFF
                     LDYYATGKLKKNEATDIVKGMVRGCINNKCSLIGGETAEMPGVYHDNDFDLAGSITGI
                     VSKNKILSSANVKKGNLLIGLPSNGLHTNGYSLVRKIFDSKSHLTKKSPGLEGDLGSE
                     LLKVHRSYLDIIQSSIKNFKINSISHITGGGIDGNTKRVIPKDLKLKIDWKSWERPVI
                     FDIIQKAGNVPESDMRKTFNLGIGLIYIVSKNVSDDFMKFLK"
BASE COUNT         3978 a         1932 c         1880 g         4108 t
ORIGIN      
        1 tagaagctgc tgcaataaca aaagccttaa tggttgccag tcctactcct tcagctccgc
       61 cgcgtgtaga gaacccaata aaacatccaa ttgaagaaat tgcaatcccg aaaaataaag
      121 ctttgatcga gcctgcaaat aaatctttaa tttcaaatga ttttttaaaa gcacctaaaa
      181 aagtttcaga aggaacaccg agaaaaaatg ttgctacaga atatgatccc aatattgcaa
      241 ttgaacttgc ataaataacc agtatcggca acataattgt agtagcaact atcctaggca
      301 ttgccagata tctgatagga tctatcgcca ttgactcaag agcatctatt tgttcagtaa
      361 ctttcattgt cccgagttca gcagccatag aagctcctac tctacctgca aggactatgg
      421 cagcaagtac aggtccaagt tcaattatga ttgcttttgt agttgcagat cccagataag
      481 aatatgagat tagtcctttg atctgatatg cagcctgcca gcaggaaaca gctcctgtga
      541 aaataccaat gatagcaaca agtgcaagag aattaactcc tacatgctgc atttgttcaa
      601 atacaagttt tctgtcccgg aatattctgg gcagactttt caaaactcca atcatcatat
      661 gaaaaaactc acctaactca gtaaaaaact gaattaattt actaccaatt ctagtgatca
      721 aactttatat gtatttaaaa ttttcattaa taactcttaa gcttttgaat tttcacctgt
      781 aataatttat tacctgattt cttcataatt gtcattacta atccctgata gtctattctt
      841 tcatatattt ccggtatatg tcctgtcact tcctgaagaa atccactcaa tgtatggtaa
      901 tcttcattat catcaggaat atcagtttcg aatttttcat taaattcttc aatatttatt
      961 tcaggattta ccaggaaaat tccttttttc tgactcttaa ttttctcagg tgtataatca
     1021 tattcgtctt cgatctcacc gacaatttct tccagtatat cttcaagagt cactagccct
     1081 tccactcccc catgttcatt tactacaata ccaagatgaa tatgtttttt ctgaaaatct
     1141 tttaatattt caccgatctg ttttgtttcg ggaataaaat gagcatgtct gataatatcc
     1201 tgtaagacaa ttacttcttt atgttcagct gcgctgatga tgtcttttga atatataatt
     1261 ccaactatat tatccagact gtctttaaaa acaggtatcc ttgaaaaacc ttcttcaatg
     1321 actttttgaa taatattctc acgcttttct tctacattaa ttgcaaacat tacattcctc
     1381 ggaatcataa catcttttgc tgtcttatca ttaaaatcaa aaaccttttg tatgatctga
     1441 gattccgtct gatcgatcct tcctgataaa gtaccttcag taattaataa tcttatctct
     1501 tcttcagaat gaactttttc tgactcagta gccggattta atcctactag ttttaaaata
     1561 taattagcgg ttccattcaa tacaagaatt gccggtttga agataaggaa aaatacctgt
     1621 aaaggaaaag aaacaaataa agtagtcggc ttaggatgtc ttattgcaat tgactttgga
     1681 gcaagctcac ctaaaataat atgtagaaaa gttattatta taaaaccaac agttaaagaa
     1741 atggtattga tagtatttgg atttactata cctaaaagaa caaataaggg atcaagcatt
     1801 ctttctgtaa caggcttacc tatccatcca agtccaagtg aagagatagt aattccaaac
     1861 tgtgttgcag ccagatactc atccagatgg gtgatctgct taattgcaag tttagctttc
     1921 ttattttctt ttatctcagt agattttatc tgagtttttc ttacttttac taatgcaaat
     1981 tctgcagcga caaataatgc atttaataat acaaaaaaaa gcagaagtaa cagatcaaaa
     2041 ataatagtct taaattcggt catagtagat ttggtaaaat aaatattatt acgactatgt
     2101 tcaatttaaa actcttagat aatctgattt ttaaagtatt gaaaaaggat ttacaaacgg
     2161 agtgtctgat atagatatat ttatttagta aatatcattt taccgatatc agttgagtaa
     2221 ctggattcaa gcttgtagaa ataaattcca cttgaaagat cactgtattt ggaagtatta
     2281 aaatcatatt tataaagtcc gggactttga aattcgtcaa caagtaaatc tacatatttt
     2341 cctgtcagac tgtaaacaga aagttttaca tttgttggaa ctaataattt atatgaaatt
     2401 atagtattag gattaaatgg gttaggatta ttctgtaaat ttacaaattc aggtacttct
     2461 gaaattccaa tttttatttc atctgatttg ataagtttat ttccaacatc atatatactt
     2521 aatttgtaaa aataaactcc gttttcagtt ggcttatcaa aatatgaata aaagtataaa
     2581 tttaaagaat ctgaaacttc ttttttcctg aaattactga ataagagatc attaacaatc
     2641 ttaaaatttt cttcacctac cttttttgat tcgatacggt atttagtgat attttgagga
     2701 ttagaaatta tgaaattaat ttctacttcg tttttattga tcgtggcatt aaataattta
     2761 acattatttt ttggatcatc acttacggat tttccaattg ctaatcccga aatgaataaa
     2821 attaataata tgtatttgaa tgatctcaaa aatttatctg tgctcctgta aaaaatctaa
     2881 tcgttttttt aatttgtctt ttggtaccaa tagtttatcc tttccaaaca ctgcttcttc
     2941 ctgatttgaa aatactaatt catattcatc actcataata attgcctctt cagggcagac
     3001 ctcttcacaa aatccgcaga agatacatct gaccatattg atctcaaatt tttccggata
     3061 tctttctttt tcattttctg tctccgctgc ctgaacttca attgcaagtg caggacacac
     3121 ccgtgaacat aatgcacatg caacacatct ttcggatccg tcatcttcct gtaccaaaac
     3181 aggtctgcct ctgtaagaag caggcggaat ccatttctct tcaggatatt gtcttgtgaa
     3241 ttttggtctg aacatctgtt taaaagtcag tcccaatcct ttaacgatct gaggtagata
     3301 agtttgttct aaaagtgtaa gatctttctt ttttattata gacattaaaa agtaaaatta
     3361 attatgcttt tcagaaattt ttataataat taaataaatg attttacttt atttaatcag
     3421 catcattttt tttgtatcag taaaattacc tgttgaaagc ttgtaataat aaatgccaga
     3481 agctaaactg ctgccattaa agtcagctct atatttacct gcatcatatc tctgattgtt
     3541 tacaagtaca gatacttcat tacccagtat atcatagact ttcaaggtta caatatcaga
     3601 tcttgtaatt gaaaattcta ttgaagtact tgggttaaat ggattcgggt aattttgtga
     3661 aagagaataa ctttcggcaa tacttgaaat attattaata cctactgtat ttacagtcag
     3721 tataaaatca ttttcaatga attgtgaagg gtttgaatct atgtacattc tgagtttgat
     3781 atgagcagtt cctaatgttg gtgcatcgaa agtaatgtcc attgtatcca actcaccggg
     3841 ctgtattgtt acaggaggct caggtccgaa tatttgattc gtatcaggaa ctacatgtat
     3901 aaaaggagca taacataatc ctacacatat agaacttgtc cattgtgcat ttggaagaat
     3961 gtcttgtgtt ctgtcgaatc ttacctggat aggtgttgta ccggaatttg aatatactgc
     4021 atatgcataa ttagcaccag tagaaccgta agttgtatct ccgccaattc tttcaaacac
     4081 catctgtgat gatgcagtaa gagatgaaat tagaaatata gttgtgatta ataataattt
     4141 tttcataata aatatgtttt aatttaataa accttgtaat ttgttaagat actgttttgc
     4201 tataagataa tcaggagaaa tatcaatagc ttttttatat tcatctactg cttcaaacca
     4261 aagacctttt gattcaaata ctctacctat atttagatgt gtaaaatgtt tgttcacata
     4321 tctgtcagct tttaaagcca gctcaagcca tgtcagagct tcttcatgct ttccttgctg
     4381 aataagatat gatccaatat cattatatgg attaccaaat tccttgtcca atgaaattgc
     4441 ttttttacat tcctcaatgg cattatcata atcacctaaa aaactataag tccagccgag
     4501 aaaagtatgt gcttcaggag ttggataaat atcgagtgaa attttataat tcagaattgc
     4561 ttcttcaaat tgtcctttaa tttgaagatc gtttgctttc tttaaatata atttggattt
     4621 atctaatggg ttagtatttt ctttagtcat aatattgttt tttatttacc taacgtgtaa
     4681 atatcgtcaa aaagtttctt taacaaacat aacttatgct attaatttta tcaagacgtt
     4741 ttattaaatt atttggaatt aatcatttac ttgcatcatt ttttaatttt gtttcctttt
     4801 ttagaaatta acataaatta tcctttatat tttgaaagat aaagtcggga tcattttggg
     4861 ctcaggacta agctcattct ctgatctgct tacagaaagc aaagttttat attcagatga
     4921 agaaagtttt cataaaatga agatcctgac cggaaaaatc tcaggaaggg aagttgtaat
     4981 atttgcagga agaaggcatt tgtatgaagg atattcaaag gaaatgatac ttgaatttat
     5041 agatattgcc aatgatcatg gagtaaaatt actcataatc tctaatgcag caggtggact
     5101 gaattgcaat tttaaagtat ccgatcttat gcttattact tctcatttaa attttttgaa
     5161 aaagaacttt attgtatcac caaatcagat ccgttacgat aatgaattat cagaaaaatt
     5221 attgaagatt gctttaagta aaaagatcaa tttgagaaaa ggtacttact gttgtctttc
     5281 aggaccgaat tatgagacaa gatctgagat cacgtattta aaaaaattcg gtattgatgc
     5341 tgttgggatg tctactattc ctgaagtttt atatgcttct gaaaaaggaa taaaaacaat
     5401 tgcaatttct tgcataacaa atttattgtc tgctagcggt attgaaatta ctgatcattc
     5461 ggaagtagta gaagccggta gatctgcttt taaaaatttt acggagataa tcatttctat
     5521 tatcgaaaat tccaattctt taattcaaaa aaacaacaat gttaactgat acacacgctc
     5581 atttatacta tccggaaata cttgataaca ttgacgaaat cctgaaccgg gcaaaagatg
     5641 caggtataga aaagatcatt gttccagctg tcgatctgga aagttctaaa aaaattatag
     5701 atctttctga aaaatatgat atgatctatg cagctttagg tgtacatccg ggtgatgttt
     5761 ctaaaaataa tttaagtgta ttagatgaaa ttgaaaaaat gctttcacat gaaaaagttg
     5821 tagccgtcgg tgaaactggt ctggattatt actgggatac ttctaatatt gataagcaaa
     5881 aagaattttt tgaaaaacag atcgagcttg cgtcagctta tgacctgcct gttttgatcc
     5941 atacaagaga atctactgac gatgcaattt caataataga atctttttta ttaaaaaaag
     6001 atcataaaaa tatcaaaggt cagtttcatt gtttttcagg taatgaaaat gaacggaaaa
     6061 gaatttcaga tctaaaagat ttttatattt cgtattgcgg aaacataact tataaaaatt
     6121 caaatttatc agatatcgta aaaaacactc ctgtagaaaa attactttct gaaacggatt
     6181 ctcctttttt accacctgtt ccattcaggg gaaaaaagaa tgaaccatcg tacatgataa
     6241 atactttgaa aaaaatttct gagttaaaat cgatggaatt atctgatctg tataaaatca
     6301 tttcagaaaa cgtattaaat ttatttttca gaaaatgatt ttaagagttt aaattattta
     6361 tgtaatgctt tatatttgtg cttattaaaa tttttcaatt aatttaatag aataaaatga
     6421 acaattacaa tttgtattat caggattcag gaagcgggaa tgtaccacca ccacctccac
     6481 caccgccttc atttaatcca ccacctccac caccgcctcc gccgtcatct tcttcagcac
     6541 ctcgatcagg atctggagga ggagcaagct ctaatgcaat atttgctctt gtacttggaa
     6601 tactttcata tataatgtgc ggtctctttg ctgctatacc tgcttggatc atgggtaaaa
     6661 aagagatcaa cgagatcaat gcaggaagat cccccgaagc cgggaagact tttgcgacca
     6721 ttggaatgtg gctcggtata ataaatgtta ttttaactgt attaaccctt attgctgttg
     6781 tatttttatt tactcttgga atattaagtt caagtgattt tgatatctga taattctgaa
     6841 aacagaatat caaaatttat taaaacaaat ttcattgaaa atatttttta aaattgctga
     6901 ttccggaagt ccggtattta ttcccgaata taaaacctcc ggatcagctg gtatggatct
     6961 ttgttcttca tccgaagaaa caatattgat cacctcatct gaatatgctc ttgttccaac
     7021 aaatttaatt atagaaattc ctgaaggttt cgaaggacag atcagaccaa gaagcggact
     7081 agcattaaat aatggaatca cagtattgaa ttctcccgga actatcgatt gtgattatag
     7141 gggtgaaata aaagtactct tgataaatca tggaaagaaa gattttgcag ttaaatcagg
     7201 tgacagaatt gcacagctca ttattgctaa atatataaaa tgtgagctga ttcaatctga
     7261 agaaatatca gattcagtgc gtggagaagg cggatatggc agcacaggaa cttcacataa
     7321 ttctgaaatt taatttccat ttatgacagg tattttaaat cttgttacaa caccgattgg
     7381 agattactca gatatgactc tgcgtgctat cagatcttta gaagagtctg ataaaatata
     7441 ctgtgaagaa tttaaagagg gaacaaaact tctcaggttt tttaatatta aaaaagaatt
     7501 atctaatctg aatgagcata atgaaactga atcaagtgaa gaacttttta tggaactgct
     7561 gcagggtaaa aatatttcaa taattactga ctgcggaact cccctctttt ctgatccggg
     7621 aaaagtaatt ttaaataaat gtatagatgc aggaattaaa gtagagtttc taggtggtgc
     7681 taattctctt ttgtcagcag ttgttttatc aggatttgat atcagcagat ttttttatta
     7741 tggattcctt tctccaaagg cagagattcg caaacagcag ttaaaagaat ttattcattc
     7801 tgaaagagtt tcagttttac tcgatgcacc ttacagactg aaagcagtat tatctgatat
     7861 cagtgatgtc tttgaagaac gaaaagtttt tgtagcattt aatgtcactc aggaaagtga
     7921 aaaaaaattc agaggttcag cagcagagat attgaatgag ataggagaag aaaatttaaa
     7981 aggtgaattc gtaataataa tagataagta taatgattaa atgtttagtt tataataatg
     8041 agaaaggtgt tacatcggtc cctcaggata aaatcaagaa atgggatttt gaatgcagtg
     8101 acgttctctg gatggatatt tataattcca gtactgaaga tcattcacag aatattcttt
     8161 cagatgtttt cgaatttcat ccgcttgcaa tagaggattc atttaagtat tacaatgata
     8221 gtaaagtaca tcatccaaag actgatgatt ttgaaaagta tttattcata gtttttaacg
     8281 gaattcaaag caccggcagc aataagaggt attccatttt ttcacttagt tgttttctcg
     8341 gtaaaaattt cattgtaact attcacaatg agtcaggaga aaactcaatt tcaaaggatc
     8401 ttaaaaattt tataaatgaa aagtctttca aaaaaggtcc tgattatatt ttaaatttaa
     8461 ttcttgatga tattgtggac agatattatc ccgtgctcga tcatatcgaa tctgaaattg
     8521 aacttactga gaataaaata ttcaaagaaa tgccgagtaa cagaaatctg atgagtatac
     8581 taaatgtaaa aaaagagtta ataaaactgc gaagaatttc ctcatatcag aaagaagtat
     8641 tatacaaact atccaaaggt gattcagagc tgatctcaat ggaagaatct ttttattaca
     8701 ggaatgtata tgatcatttg gttagggttt ccgatactac agagtcttac agagattacg
     8761 tctcgggaat tcttgattca tatttatcta tagtaaataa taagatgaat gaggtaatga
     8821 aatttctaac aatcattgct accataatgc ttcctctgac acttatcacg ggaattttcg
     8881 gaatgaattt tgactctatt ccatttctac ataatgaaac aggtttttat atcagtttga
     8941 cctttatgtt tattgtagct gcaattatgc tgatgtggtt taaatataaa aaatggatct
     9001 gaactaaaat ttttcaaaaa tccaacgtct caccttcagt ttcagtcttc agtttgtatg
     9061 cacttaaaat ttcagccagc acatttgagt cagatctgtt ctcggtcttt cttccgtcaa
     9121 tttcagttac aggagtcaac tcccccatcg taccggttgt aaatacttca tcagcattat
     9181 aaaattctga aactgaaata tttctttcaa aacaatcaat ctttaacttt gaagctattt
     9241 ccattacaag tccgcgcgtg ataccaggca gacacgagtc agcattcgga gtgtaaagcg
     9301 ttccgttttt caccataaaa atattagtag catttgtttc tgatacatac ccgttcacat
     9361 caagcatcag agcatcatct gctccacaca tgtttgcttc tatctttgca agtatgttgt
     9421 tgatgagatt gttgtgatga atttttgaat caagattctg aggagaattt cttcttatac
     9481 ttgatgtcac aagtttaatt ccttctgaat tatcgtatac aggttttttc cattctgcta
     9541 aaactattaa gcaaggtccg tgtgtattga gtctcggatc cattcctgaa gtaattttct
     9601 cacctcttgt aagtgtaagc cgaatatgtg ctccgtcttt cattccgttc gctttgagag
     9661 tttccattac agcttttttt atgaaatctc tgtcaggaat atttttgaaa tctaaagtct
     9721 ttgcagactc gtgaagtctg tttacatgtt tatcaaaaca aaaaatcttt ccgtcatata
     9781 ttctaagtcc ttcccagact gcatcgcctc cctgtaccga gctatcgaag actgacactt
     9841 ttgcgtctgc tctgtcataa agtctgtcct ttataaatac ttttatgtta ctgtttcttt
     9901 catcaaattt ctgaagcata attttaagct ttaattgaaa aatcttttaa ataattataa
     9961 tactttttac attcaatata cagcagtatg aatttttccg gaagctcttc atcttttctg
    10021 aatggtctgg aaaatccagt cgacttatga acattctcat accagtactt cgcccagact
    10081 ccgtcttcag ctataggacc tgcattccat ctcagcatat ttttttgaaa aggaattccg
    10141 agcttttcac acaattctct caaaactttt tcaggatctt ttaaaatttc tcctgaatct
    10201 ataactatag attcctgacc atcggaaaat aatttttgaa aaagatcata ttgctttttc
    10261 acaccaattc tttccatcgt gacttcagga attacttttg caaatgagat aataagttct
    10321 ttcggatctc ggataagaaa aacattaatt acatctttta aaaaatcttc attgagttct
    10381 ataagatgat gtgtcatttg tttaaagaat aaaacctctt tgtcatattt accatccaca
    10441 atatctgaaa tcactttctc accatcggct ttcatgcttt taataatttc atctttaccc
    10501 ggatgatctg aatctgtgat ccttagatag tgtccgtaca atggttcgtc aaaaactttt
    10561 gtatcacttc tctgtgcaaa tgaatacatc agagctgtag aaatatttcg cggacttgac
    10621 cagagacaga ttcgtttcaa gatatgattt ataaatttga gtctaaatta tcgatacaaa
    10681 tttcaatatt caattcttaa acttctttaa aatatttttt atttatatca tatgcgtttg
    10741 gaaacgagcg ggaaatctga ccaaaaaaac tataacagat atttaaaaaa atcaaaaaaa
    10801 aatccttata gtctttgaaa ctttgggtca aagatcgcgg aaataaaaaa ttatttttcc
    10861 aaaaatagtt ttaaactgtt taaaccgtct tttcagtttt agtaattcat tgaaatacag
    10921 gcttgaattg gctattttga caggtgaaag tcagctataa gtcttcaggt gttaatatat
    10981 ctgaagccga caaattcgtc gaaaatatca aacccctagt caaagaaact tttacaaaaa
    11041 acgttttaaa aggaatcgga aactttggtg ctttttatga aatagatatt aagaagcata
    11101 atcatcctgt ttttgtatcg agcgttgacg gtgtgggaac gaaacttaaa attgcctcgg
    11161 cattaaaaaa gtttgacaca attggtgaag atcttgtgaa tcattgtgta aatgacattg
    11221 cagtgtgcgg tgctgttccc ctattctttt tagattatta cgctacagga aagctgaaga
    11281 aaaatgaagc aactgatatt gtaaaaggaa tggtcagagg ttgtataaat aacaaatgtt
    11341 cattgatcgg cggagagact gcagaaatgc cgggagttta tcatgataat gattttgatc
    11401 ttgcaggttc cattacaggt atagtttcaa aaaataaaat attgagttcc gcaaatgtaa
    11461 aaaaaggaaa tttgctgatc ggtcttccgt caaatggtct tcacacaaat ggctattctc
    11521 ttgtcagaaa aatttttgat tctaaaagtc atttaacaaa aaaatctcct ggtcttgaag
    11581 gagatctcgg aagtgaattg cttaaagttc acagatctta tttagatata attcagagtt
    11641 cgataaagaa ttttaagata aattccattt cacatattac tggcggtggt attgatggaa
    11701 atacgaaacg agtaattccc aaagatctga aactaaagat cgactggaaa tcatgggagc
    11761 gtccggtaat ttttgacata attcaaaaag ctggtaatgt acctgaatct gacatgcgaa
    11821 agacttttaa tctgggaatc ggattgatct acattgtatc aaaaaacgtg tcggatgatt
    11881 ttatgaaatt tctcaaaa
//
LOCUS       DAORVQ010000099        11888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2630_length_11888_cov_4.357813, whole genome shotgun sequence.
ACCESSION   DAORVQ010000099 DAORVQ010000000
VERSION     DAORVQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2630_length_11888_cov_4.357813"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1782
                     /locus_tag="PLX80_09075"
     CDS             274..1782
                     /locus_tag="PLX80_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQY20979.1"
                     /translation="MKTLTLIFLFFTAISYGNYSTPGTGITWNLDSLVANSAGNVTNS
                     GGIYFVNDTITVSANDTIKILNAAELRLGTSVFIDIFGVMIANPVNPADSVKITAQDT
                     VVKYLGLKFEDQSDGSLLRNVIFEYGNSIRMLDCNITIDKCVLRYNTLSSSFGSGAIS
                     LFRSNSIISNCKIFRNRRAAITSGANIASSPQILNNHIYENNTDNANVPQINFGATGP
                     EQMVIRGNTITGLFTNCGGISFLPVGSIPDAVIENNIIKHNRYGIAIQGGPSNILIKG
                     NIIDSNNIQGNPALGGSGINFNGTSTQVTIVSENIIRGNLWGVTIQNTAKPNLGDSIN
                     LGFNELYDNGNSGKTFDLFNNTPDTIMAENNYWGTDNIDSVEAHIFHNPDSSALGFVD
                     FMPIRIITGINQSSINIHQKSFQLFDAYPNPFNPSTVIRFSIHENKNGSEFYNLRIFD
                     ITGRQISELINGEIRPGNYSVTFNAADLPAGVYLYRLSSGSYSVSKKLMLIK"
     gene            complement(2029..2361)
                     /locus_tag="PLX80_09080"
     CDS             complement(2029..2361)
                     /locus_tag="PLX80_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20980.1"
                     /translation="METHHSPDKKHIAKGILIGFIILILAAVAAGSAFLMVSVIVMGC
                     VKSPPEWVYMLVLFGFPIPLIMTSIVVPYLYIKKQKVIWMVLTPVAGIILSCMIFFVW
                     FLILTRYC"
     gene            complement(2631..3371)
                     /locus_tag="PLX80_09085"
     CDS             complement(2631..3371)
                     /locus_tag="PLX80_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQY20981.1"
                     /translation="MNFTLKVWRQKNKNSEGKLVTYPVKDVSPDMSFLEMFDYLNEDL
                     INKGEEPIAFDSDCREGICGTCSMWINGRAHGPVPGVTTCQLHVRSFKDGETIVVEPW
                     RAKAFPVIKDLVVDRSSFDKVIAAGGFVSINTGGAQDGNDILISKEDAAEAFSASICI
                     GCGACVAACKNSSAMLFVASKISHFAVLPQGKVEAQNRVQNMVAKMDEEGFGDCSNTY
                     ACEAQCPKNISVEKIAKMNREYLTAKLI"
     gene            complement(3503..5482)
                     /locus_tag="PLX80_09090"
     CDS             complement(3503..5482)
                     /locus_tag="PLX80_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HQY20982.1"
                     /translation="MSDIHSKIPEGPLESKWTKYCSTVPLVNPSNKRNLEIIVVGSGL
                     AGASAASSLAEQGYKVKVFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTI
                     KGGDYRSREGNVYRLAEVSGNIIDQCVAQGVPFAREYGGLLSNRSFGGTQVQRTFYVA
                     GQTGQQLLLGAYNSMERQIGIGSVQMFSRHEMLDIVKIDGKARGIIARDLISGKLERH
                     FGHAVLLCTGGYGNAFYLSTNAMGSNVTAAWKAHKQGAYFANPCYTQIHPTCIPVSST
                     HQSKLTLMSESLRNDGRIWVSKKKDDTRKANDIPEEERDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGHGVGASKMAVYLDFASSMKRYGTIEAHKKNIHNASDEEINKLGKEVL
                     KEKYGNLFDMYMQITDENPYEVPMRIYPAVHYTMGGLWVDYNLMTNVPGLYALGEANF
                     SDHGANRLGASALMQGLADGYFVIPYTIGVYLSDEIRTKPISTDDNAFVEAEKKVEEQ
                     INKLMSIRGTKSVDHFHKTLGLILWNKCGMSRSEAGLKEAIEEIKTLRENFWSDVRIP
                     GEANELNTELEKAGRVADFLELGELMCVDALHRNESCGGHFREEFQTEEGEAKRDDEN
                     FVYVAAWEKNGNDSWDLHKENLEFNEIHISKRSYK"
     gene            complement(5530..6225)
                     /locus_tag="PLX80_09095"
     CDS             complement(5530..6225)
                     /locus_tag="PLX80_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HQY20983.1"
                     /translation="MTTFLSGIFSKSVGKKFAMGITGLFLISFLIVHCAINATIFLND
                     GGNLFNVTAHFMGTNIFIRTMEIVLFAGIIWHIVQALIVTRDNKKANSIKYNVFDGKA
                     NSRWYSRWMGLLGTLILMFLILHLLHFWLQTRFTGLGLPPVMIDGKEYENIYGKMLEV
                     FSIGWVVVVYVLGMISLAYHLLHGFQSAFQSMGLNNKKYTPLIKLTGKLFSILIPLIF
                     ALMPIAIYFGFLK"
     gene            complement(6547..6861)
                     /locus_tag="PLX80_09100"
     CDS             complement(6547..6861)
                     /locus_tag="PLX80_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQY20984.1"
                     /translation="MKIQINTDNHISGTREKTDPIIETISISLKRFSERITRIEVHLS
                     DENSKKKGQKDIKCVLEARLKGMDPVAVTDHEDRVEDAVDGALDKMTSKLDTILGKLK
                     SF"
     gene            complement(7093..7470)
                     /locus_tag="PLX80_09105"
     CDS             complement(7093..7470)
                     /locus_tag="PLX80_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20985.1"
                     /translation="MSNKLLIGLVSGIAAGVAITLIVRNRGTENFMEDDYFDPNEELG
                     NASKFLSTAKKKAEDLVTEAKERSGILLDEANEILKNAKEKVRSIGENTIDDPEKELK
                     SIKEIIEEKIEKLNDKLSDEDNS"
     gene            complement(7489..8034)
                     /locus_tag="PLX80_09110"
     CDS             complement(7489..8034)
                     /locus_tag="PLX80_09110"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HQY20986.1"
                     /translation="MFKPTKLFITKGVGRHKDYLQSFELALRNAGIEKSNIVTVSSIF
                     PGGCKRITKAQGVALLEPGEITFCVMARNFTNEPNRLIASSIGIALPKDDSHYGYISE
                     HHPYGEPEKVSGEYAEDLAATMLATTLGIEFDSETAWNKRENVYKQSGKIIKTFNVTQ
                     SAEGDKNGLWTTVIACVVFLK"
     gene            complement(8056..9267)
                     /gene="tyrS"
                     /locus_tag="PLX80_09115"
     CDS             complement(8056..9267)
                     /gene="tyrS"
                     /locus_tag="PLX80_09115"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQY20987.1"
                     /translation="MFPSVKEQFDIIKTGTVEIIPEEELLKKFEISLKKNIPLNIKLG
                     CDPSSPDLHIGHSVVLNKLRQFQDLGHNAILIVGDYTAMIGDPSGKKKTRPQLTFEET
                     RENGVSYFEQAGKILDLDKTKVVYNSEWLSKLDLKDMIEILSKFTVQRILERDDFTNR
                     LKEHTEISFHEMLYPVMQGYDSYAIDADVELGGTDQKFNNLVGRDIQKRYGKEQQIVI
                     TMPLLVGTDGYEKMSKSLDNAIGISDEPKDIFGKTMSIPDTLIYDYFELCTRVDKKEL
                     KSIKETLEDKNNNPRDLKRRLGIELVKQYYDEKIATKTIEEFDEIFIKKGIPDEIPEM
                     NFDQEKMKLIEIMTKGNLAESNSNARRLIEQGAVSIDSKKITDVNSEIDIINGMIFKV
                     GKRKFLKLIKQ"
     gene            complement(9268..9720)
                     /gene="smpB"
                     /locus_tag="PLX80_09120"
     CDS             complement(9268..9720)
                     /gene="smpB"
                     /locus_tag="PLX80_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQY20988.1"
                     /translation="MNQGTDKIKIIVTNRKANFEYEIISRYEAGIVLKGTEVKSLRAG
                     KANLQESYGRIKDNEVWLVNSHINEYSFGNLNNHEPNRSRKLLLNKKEIRKIRQMLQE
                     KGLTLVPLKLYFKNSNVKAEIGIARGKKLYDKRESIKKREVERNLSRI"
     gene            9769..10410
                     /locus_tag="PLX80_09125"
     CDS             9769..10410
                     /locus_tag="PLX80_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY20989.1"
                     /translation="MKIKQFELNPFSMNCYLYYDEENKDGVIIDPAAFTEEEKKQITD
                     FVESNRIKVKYIINTHGHLDHILGNNFAKNYFNVPVYMHKDDNFMLKESKEQARFFGL
                     DFPEPPPTDVFINEDTVLTINDTDLTFINTPGHSPGSVCIIDHKNKNVFCGDLIFNKA
                     IGRTDLQGGDLNTLISSVKNKLFKVCSDDYKLYPGHMDDTNIKDEKSSNPFLI"
     gene            complement(10418..10936)
                     /locus_tag="PLX80_09130"
     CDS             complement(10418..10936)
                     /locus_tag="PLX80_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20990.1"
                     /translation="MKTLSILAMILFIFSGNVFSQDKININNEDYKFKFNLPAGWTER
                     KVEETNKNDAISYTFDRDDGKIAIMLLAFKVNEVKNLDDFVYTLEKDLTLNIPKKDGS
                     YKDFDSGNYDGKSCLYQDSEFTEILYYYRTKIAEGNNYTYLLRFIVPSSRYHSEIDAD
                     IRKLAEKFTPDV"
     gene            complement(10964..11821)
                     /locus_tag="PLX80_09135"
     CDS             complement(10964..11821)
                     /locus_tag="PLX80_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQY20991.1"
                     /translation="MQKHFKIIKKNFNGYLTLLLISAVIILSGVYSCGSNMYSVQDDV
                     KLGEEVNQQIKDDPKQFPVMVGNQAVKDYVSGLGNYVLNNSPYIKYKNVFPYTFYVIH
                     DDSTVNAFCLPGGYIYVYTGLIKFLDNEAALAGVIGHEIAHAERRHMTQRLTSYYGVS
                     VVLGLILGNNPSAFGEIAANLFVGLGFLANSRADEEDADNFSINYLKSTKYFPGGILD
                     FFEKIRLEQSKKGQTPGDLERLLATHPLPMDRIENVHEKLNKISPKPDSTKGLFRNEY
                     NLMKEKLPK"
BASE COUNT         3695 a         2306 c         1781 g         4106 t
ORIGIN      
        1 attacattta taattttaag tacctattac tattttcaac ccctcccaaa cctcccacta
       61 tctaagggaa gggcaagtgt ggggtattta gaaggaatat ttacatacaa agatttatgt
      121 gaatcgagcc gttgttggtt tatataaaag ttttttggca ggttttgaga tagaaaattc
      181 attcaattaa ctttttggct accgggatcc ttagactaaa tatattttta aattcaaata
      241 tcaataaaac tttttaataa taatacaaaa ctcatgaaaa ctttaacact gatattttta
      301 tttttcacag caataagcta tggaaactat tctacacccg gtacaggcat aacctggaac
      361 ttagacagtc tcgtcgctaa ctctgccggc aatgtcacaa attccggcgg tatttatttt
      421 gtaaatgata ccattactgt ttcagcaaat gatacaatta aaatattgaa tgctgctgaa
      481 ttaagattag gaacttcagt attcattgac atatttggtg tgatgattgc aaaccctgtc
      541 aatccggcgg actcagtaaa gatcactgct caggacactg ttgttaaata tcttggtctg
      601 aagtttgaag atcagtcaga tggatcgctt ttgagaaatg taattttcga atatggaaat
      661 tcgatcagga tgctcgactg caatatcact attgataaat gtgtactcag atataatact
      721 ctaagcagtt ctttcggcag cggtgcgatc tcacttttca gatcaaattc aattatttca
      781 aactgtaaaa tattcaggaa cagacgagct gcaataactt caggtgcaaa cattgcgtct
      841 tctccgcaga ttttaaataa tcatatttat gaaaacaata ccgataatgc caatgtacct
      901 cagataaatt ttggtgctac aggtccggag caaatggtaa tcagaggaaa tacaatcaca
      961 ggacttttta caaactgcgg gggaatttct tttttacctg ttgggtctat tcctgatgct
     1021 gtgatcgaaa acaatattat aaaacataat cgatacggta ttgcaataca gggcggaccg
     1081 tcaaatattc tcatcaaagg aaatattatt gacagcaata acatacaggg aaatcctgca
     1141 ctcggaggca gcggaattaa ttttaacggt acttcaactc aggttacaat agtctctgaa
     1201 aatattatca gaggaaatct atggggtgtt acaattcaga atacagcaaa gccgaatctg
     1261 ggagacagca taaatttagg attcaatgaa ttatatgaca acggaaattc cggaaagaca
     1321 tttgatcttt ttaataatac acctgacaca ataatggctg aaaataatta ctggggaaca
     1381 gataatatag attcagtaga agcacatatc tttcataacc ctgacagctc agcattggga
     1441 tttgttgatt ttatgccgat cagaattatt acaggaataa atcaaagcag cataaatatt
     1501 catcaaaaaa gttttcaatt atttgatgca tatccaaatc cgttcaatcc atctacagtc
     1561 atcagattca gcatccatga aaataagaat ggatcagaat tttacaattt aagaatattt
     1621 gacattaccg gaagacagat ctcagaatta ataaacggag agatacgccc gggtaattac
     1681 tcggttactt ttaatgcagc agatctgccg gcgggtgtgt atctgtatcg cttgagcagt
     1741 ggaagttata gtgtatctaa gaaattgatg ttgattaaat aatttgtgta acagaattta
     1801 aaagtggtct tttttgattt atcattatat tttaaattta ctttctaacc taatgacatt
     1861 acagtttgct caccattctg tcattcaagc gagctcctaa ctggaatcca gttataacgg
     1921 aaactaaaaa gaattgtaaa cctagagtaa atgactatat aaattcaaga acacatgcgt
     1981 tccaaaaaat ttgcgaatta ttaaatagat tctcttacac tccccttctc agcaatatct
     2041 tgtaagaatt aaaaaccaaa caaagaatat catacagcta agaattattc ctgcaacagg
     2101 agtgagtacc atccatatta ctttttgctt ttttatatat aaataaggta caacaattga
     2161 tgtcatgatg agaggaatgg gaaatccaaa taaaactaac atataaaccc actcaggtgg
     2221 actctttaca catcccatta caattactga aaccattaag aaagcacttc ccgcagcaac
     2281 agcagccagg ataagaataa taaatccgat taaaatacct tttgcaatat gcttcttatc
     2341 cggtgaatgg tgtgtttcca ttattgtatt ttttatgatt gcaaaaaata tttagaaaca
     2401 tagtcagcga ccaaaagatt ttgatggtga cgttgaacat ttattctctg caaagaaact
     2461 ttttgaaaca cctgctatga cgaatacact acaaattttt ggtccccacc taactaaatt
     2521 ccatagaacg ttagacgaac ttgtaatgct ctaggaaacc aaacacatct atactttcat
     2581 tgtaaaattc tgtggatact tcataattaa attcatgtca aagatttaga ttatattaat
     2641 ttagcagtta aatattctct gttcatcttt gcgatctttt ccacggaaat atttttcgga
     2701 cattgtgctt cacatgcata agtattagag caatcaccga atccttcttc atccattttt
     2761 gcgaccatat tctgtacccg gttttgtgct tctacttttc cctgcggtaa cacagcaaaa
     2821 tgcgaaatct tagatgcgac aaataacatt gccgaagaat ttttacatgc agccacacat
     2881 gcgccgcagc caatacaaat agatgcgctg aaggcttctg cagcatcttc tttggatata
     2941 aggatatcat ttccatcctg tgcgcctccg gtatttattg aaacaaaacc tccggcagca
     3001 atgactttat caaacgatga acggtcaaca acaagatctt taataactgg aaaggctttt
     3061 gctctccatg gctcgactac gatcgtttca ccatctttga aagaccgaac gtgtaactga
     3121 caggtagtca ctcccggaac aggaccatgc gccctgccgt tgatccacat actgcacgta
     3181 ccgcaaattc cttcacggca atcactgtca aatgcgattg gctcttcacc tttgttaata
     3241 agatcttcat tcagataatc aaacatttcg agaaatgaca tatccggaga tacatctttc
     3301 acaggataag tcactaactt accttccgag tttttatttt tctgacgcca tactttaaga
     3361 gtaaaattca tgttctctat ataataaatt atattaaatt atagatttta aattctgttt
     3421 attatttaat atctgcagat tttttttcac aatcataacc ttaatagaat atgatcctca
     3481 attaaagtct ctgactttta aattatttgt aacttctttt tgaaatatgt atctcattaa
     3541 attccagatt ttctttatgc agatcccagg aatcatttcc gtttttttcc caggcagcaa
     3601 cataaacaaa attttcatca tctcgttttg cttctccttc ttctgtctga aactcttcac
     3661 gaaaatgtcc gccgcagctt tcattccggt gcaatgcatc tacacacatt aattcaccaa
     3721 gttctaaaaa atccgcaacc cttccggctt tttccagttc agtatttaat tcatttgctt
     3781 ctccgggtat gcgaacatct gaccagaaat tttcacgcaa agtttttatt tcttcaatag
     3841 cttctttcaa tcctgcttca cttcggctca ttccgcattt attccatagg atcaatccaa
     3901 gcgttttatg aaaatgatca actgactttg ttcctctgat gctcatgagt ttattgattt
     3961 gttcttctac ttttttctca gcttctacaa acgcattatc atcagtagat attggttttg
     4021 tacgaatttc atctgaaaga taaactccaa ttgtatatgg tatcacaaaa tatccgtctg
     4081 caagtccctg catcaatgcg gacgcgccaa gccggttagc accgtgatca gaaaaattgg
     4141 cttctcccag cgcatataat cccggaacgt ttgtcataag attataatca acccacaaac
     4201 ctcccatagt ataatgcaca gcaggatata tccgcatcgg aacttcataa ggattctcat
     4261 ccgtaatttg catatacata tcaaataggt taccatattt ttctttcaaa acttccttac
     4321 ctaatttatt tatttcttca tcagaagcat tgtgaatatt ttttttatga gcttcaattg
     4381 ttccgtaacg cttcatgctt gatgcgaaat ccagatatac tgccatctta gatgcgccaa
     4441 ctccgtgacc ggcatcgcac ctttccttgg cggcgcggga tgcaacgtcc ctcggcacga
     4501 gatttccaaa agccggatat cgtctttcga gataataatc gcgttcttct tcagggatat
     4561 cgtttgcttt acgtgtatca tcctttttct ttgagaccca gatccttccg tcattacgca
     4621 atgactcact catcagggta agctttgact ggtgagtact gctgaccgga atacatgtag
     4681 gatgaatttg cgtgtaacag gggtttgcaa aatatgctcc ctgtttatgc gctttccagg
     4741 ctgctgttac attgctcccc attgcatttg ttgacagata aaatgcattt ccgtaacctc
     4801 ctgtacacag caatacagca tgtccaaagt gacgttccag ttttccggaa atcagatcac
     4861 gggctatgat gcctcttgct ttgccatcga tcttaactat gtcaagcatt tcatgacgtg
     4921 aaaacatctg tactgaacct attccgatct gacgctccat tgaattataa gcacccagca
     4981 gtaactgctg acctgtctga cctgcaacat aaaatgtacg ctgtacctgt gttcccccaa
     5041 aagagcggtt gcttaataaa cctccatact cccttgcaaa aggaactccc tgtgctacac
     5101 actgatcaat tatattaccg ctgacttctg caagtctgta tacatttcct tcacgcgatc
     5161 ggtaatctcc tcctttaatt gtatcataaa ataatctgta cacagagtca ccgtcgttct
     5221 gataattctt cgcggcatta attccgccct gtgccgctat actgtgagcc cgtctgggtg
     5281 aatcctgaaa acaaaatacc tttactttat atccctgctc tgctaaagat gatgcggcag
     5341 atgcgcctgc taatcctgat ccaactacta tgatctcaag gtttctttta tttgaagggt
     5401 taacaagcgg tacagtagaa caatattttg tccatttact ttcaagagga ccttcaggaa
     5461 ttttgctatg gatatctgac ataattaata tttgatataa ttaggtataa gaatttttat
     5521 ttttaaatat tatttcaaaa aaccaaaata tattgcgatt ggcattaaag caaaaattaa
     5581 cgggatcaaa atggaaaata attttccggt cagcttaatt aaaggcgtat attttttatt
     5641 atttaatccc attgactgaa aagccgactg aaacccatgc aacaaatgat atgcaagtga
     5701 aatcattccg aggacataaa caactaccac ccagccgata gaaaatactt caagcatttt
     5761 tccatagatg ttttcatact cttttccgtc aatcattaca ggcggtaagc ctaatccggt
     5821 aaaacgtgtt tgaagccaga agtgtaaaag atgcaagatc agaaacatca aaatcaatgt
     5881 tccgagtaat cccatccatc tggaatacca tctgctgttt gctttgccgt caaagacatt
     5941 atactttata gagtttgctt tcttattgtc tcttgttacg atcaaagcct gtacaatgtg
     6001 ccatataatt ccggcgaata atactatctc cattgttcta ataaagatgt tagttcccat
     6061 gaaatgcgca gtgacattaa ataaattgcc tccatcgttc aaaaatattg tggcgtttat
     6121 agcgcagtgt acgatcagaa aagaaatcag gaaaagtccc gtaataccca ttgcaaattt
     6181 cttacctaca gatttactaa agatcccgct taagaatgtt gtcattaatt aattttatca
     6241 gttataatta caattttcaa aaatcttaac tctgtattta tttgaagttt attttaaaaa
     6301 tactttttaa tattttggtt tctcagttaa actgatctct aattcctatc cggaggattg
     6361 aaattactaa gttattttta cgccgtattt aatttaacta aaggcttaac tttaatattg
     6421 aaaaagcatg aattaaattc gtattaaaac gatttttcgt agaatatgaa attcttaact
     6481 aaaagaaata cttaaacagc aactatggct cattgtaata tatcagggtt taaatatttt
     6541 ctcattttaa aagcttttta atttccctaa aatcgtgtct aacttcgaag tcattttatc
     6601 caatgcacca tctacagcat cttcaactct gtcttcatga tccgttactg ctactggatc
     6661 cattcctttc aatctcgctt cgagtacaca cttaatgtct ttctgtcctt ttttcttgct
     6721 gttctcatcg gaaagatgaa cttcaattcg cgtaatcctt tcactaaatc tttttaatga
     6781 aattgatatc gtttcaatta ttggatcagt cttttcccta gttcctgaaa tatgattatc
     6841 ggtattaatt tggattttca ttagattata tttttattga agaaatgagg ttatttaaaa
     6901 ttgtgtgata aataaaattt cgagcaggtg gttctgttgt tggctgctga aaaatttttt
     6961 tggaaatgaa tcgcaaaatt caaatgagat aaaaaaactt tttgtaaaat catcaaagtc
     7021 gaatttagca attggtgtta aaatcctaaa aaaattttga aacaattaaa gatggcacag
     7081 atcaaacgtc aatcaggaat tatcttcgtc agataattta tcattgagtt tttcaatttt
     7141 ctcttcaatt atttctttga tagactttaa ttctttttct ggatcatcaa ttgtattctc
     7201 accaattgat cttacttttt cttttgcatt tttgagaatt tcatttgctt cgtcaagaag
     7261 aatacctgat ctttcttttg cttcagttac aagatcttca gctttctttt ttgcagttga
     7321 cagaaattta ctggcatttc ctaattcctc attaggatca aaataatcat cttccatgaa
     7381 attttctgtt ccgcggtttc ggacaattag tgtaatagct actcctgctg ctataccaga
     7441 cactaaccct attaataatt tatttgacaa atcagttata tttaattttt attttaaaaa
     7501 tactacacag gcaattacag ttgtccataa tccgttctta tcaccttctg ctgactgtgt
     7561 tacgttaaaa gttttaatta tcttaccgct ttgtttatat acgttttctc ttttattcca
     7621 tgcagtctct gaatcaaatt caataccaag agtagttgca agcattgtcg cagcaagatc
     7681 ttctgcatat tcaccggata ctttttcagg ttcgccgtaa ggatgatgct cggaaatata
     7741 tccataatga ctgtcatctt taggcagagc aataccaata gaagatgcga ttaatctgtt
     7801 aggttcattt gtgaaatttc ttgccatgac gcaaaatgtg atctcacccg gctctagcaa
     7861 agctacacct tgagcctttg taatcctttt acagccgccg ggaaaaatgc ttgatactgt
     7921 aactatatta cttttttcta tacctgcatt tcttaatgca agttcaaaag actgaagata
     7981 atctttatgt cttcctacac ctttggttat aaataatttt gttggtttaa acaattttat
     8041 tctccttttt aaagcttact gtttaattaa ttttaaaaat tttcttttac ctactttaaa
     8101 gatcattccg tttattatat ctatttctga atttacatct gtaatttttt tactgtcaat
     8161 actaactgct ccctgctcta ttaatcttct cgcatttgaa tttgactccg caagatttcc
     8221 ctttgtcatt atctcaatca acttcatttt ttcctgatca aaattcatct caggtatttc
     8281 atcaggaatt cctttcttaa taaatatctc atcaaattct tcaattgtct ttgttgctat
     8341 cttctcatca taatattgct tcacaagttc aattccaagt cttcttttta aatcccttgg
     8401 attattattc ttatcttcaa gagtttcttt tatcgacttc aattcctttt tatcaactct
     8461 cgtgcaaagc tcaaaataat catagatcaa agtatcagga atactcatcg tctttccaaa
     8521 tatatctttt ggctcatctg aaatgccaat tgcattatca agcgacttgc tcattttttc
     8581 ataaccgtca gtgccgacaa gcaaaggcat tgttatcaca atctgttgtt ctttaccata
     8641 tctcttctga atatcacgac cgacaagatt attaaatttc tgatcagtgc caccaagctc
     8701 tacatcagca tctatagcat aagaatcata accctgcatt acaggataca gcatttcatg
     8761 aaaagaaatt tcagtatgct cctttaatct gtttgtaaaa tcatctcttt cgagaattct
     8821 ctgaactgta aacttgctca gaatttctat catatctttc agatcaagtt tcgaaagcca
     8881 ttcagaatta tagacaactt tagtcttatc cagatctaaa atttttccgg cttgttcaaa
     8941 atacgaaaca ccattttctc tggtctcttc aaaagtaagc tgaggtcttg tcttcttttt
     9001 acctgaagga tctccgatca tagcagtata atcacctact attaaaattg cattatgtcc
     9061 aagatcctga aactgtcgta gtttatttaa tactacagaa tgtccgatat gcagatcagg
     9121 actggacgga tcacaaccca gctttatatt taaaggtata ttctttttta aagaaatttc
     9181 aaactttttt aaaagttctt cttcaggtat gatctcaact gtacctgttt ttataatatc
     9241 aaattgctct tttactgatg gaaacattta aattctgctc agatttcttt caacttcgcg
     9301 tttcttgatc gattcgcgct tatcatataa ttttttacct cttgcaattc caatttcagc
     9361 ttttacattt gaatttttaa agtacagctt taaaggtaca agtgtcagcc ctttttcctg
     9421 aagcatctgt ctgatctttc tgatctcttt tttattcagc aataattttc tgctgcggtt
     9481 aggttcgtga ttgtttaaat ttccgaaact gtattcatta atatgagaat tcacaagcca
     9541 tacttcgtta tcttttattc ttccataaga ttcctgcaaa tttgccttac cggctctgag
     9601 tgattttact tcagttcctt ttaatacaat tccggcttca taacgtgaaa taatttcgta
     9661 ttcaaaatta gctttacggt ttgttacaat tatcttaatt ttgtcggttc cctgattcaa
     9721 gtgtatgcaa attaaaattt aaaaaaagta aaatcaatta acattatgat gaaaattaaa
     9781 caatttgagc taaatccatt cagtatgaac tgctatcttt actatgatga agaaaataaa
     9841 gacggtgtaa taattgatcc ggctgctttt actgaagaag aaaaaaaaca gatcaccgat
     9901 tttgttgagt caaacagaat taaagtaaaa tacattatca atactcacgg acatcttgat
     9961 cacattcttg gaaacaattt tgctaaaaat tatttcaatg tacctgttta tatgcataaa
    10021 gatgacaatt tcatgttaaa agaatcaaaa gaacaggcaa gatttttcgg actggatttt
    10081 cccgaacctc cgccgacaga tgtttttata aatgaagata ccgtactgac tataaacgat
    10141 actgacttaa cttttataaa tacaccgggt cattctcccg gaagtgtctg cattattgat
    10201 cataaaaaca aaaatgtttt ctgcggtgat ctgatattta ataaagccat cggcagaact
    10261 gatctgcagg gtggtgatct taatactctt atcagttcag taaaaaacaa attatttaaa
    10321 gtctgctcag atgattacaa attatatccg ggtcacatgg acgatacaaa tatcaaagat
    10381 gagaagagtt caaatccatt tctaatctga gtatttttta aacatccgga gtaaattttt
    10441 ctgcaagttt tcttatatct gcatcaattt cagaatggta tctgctcgaa ggtactatga
    10501 atctcagcaa ataagtataa ttatttcctt ccgcgatctt tgtcctgtag taatacaaaa
    10561 tttccgtaaa ttctgaatcc tgataaagac agcttttgcc gtcataattc cctgagtcaa
    10621 aatctttata tgagccgtcc tttttaggaa tgttaagcgt aagatctttt tctaatgtat
    10681 atacaaaatc atcaagattt tttacttcat tcactttaaa agcaaggagc attatagcta
    10741 tctttccgtc atccctgtca aatgtatatg atattgcatc gtttttattt gtctcttcga
    10801 ccttcctttc agtccagccg gcaggtaagt taaatttaaa cttataatct tcgttgttaa
    10861 tatttatttt gtcctgagaa aaaacattcc cggaaaaaat aaacaggatc attgcgagta
    10921 ttgataaagt tttcattata tttttttaat tattttaatt ttattatttg ggtaatttct
    10981 ctttcatcaa attatattca ttcctaaaca gccctttagt agaatcaggt tttggactaa
    11041 ttttatttaa tttttcgtgt acattttcaa tcctgtccat tggaagagga tgagtagcta
    11101 aaagtctttc cagatctccg ggagtctgac cttttttact ttgctccaat cttatttttt
    11161 caaaaaagtc taatatacca cccggaaaat atttagtact ttttaaataa tttatggaaa
    11221 aattatctgc atcttcttca tcagccctgc tgtttgcaag gaatccgaga cctacaaata
    11281 aattagcagc aatctctccg aatgcacttg gattgtttcc aagaattaaa cccagaacca
    11341 cactgactcc ataataagat gtcagacgct gagtcatatg ccttctctca gcatgtgcga
    11401 tctcatgacc tattacacct gcaagtgctg cttcattatc aagaaattta atcaaaccgg
    11461 tatatacata aatatatccg ccgggcaggc aaaatgcatt tactgtgctg tcatcatgta
    11521 tcacataaaa agtatatgga aacacatttt tatatttaat atatggagaa ttattcaaca
    11581 cgtaattacc aaggccgctg acataatctt ttacagcttg atttccaacc attacaggaa
    11641 actgtttcgg atcgtctttg atctgttgat ttacttcttc tcccagctta acgtcatcct
    11701 gcaccgaata catattagat ccgcagctgt aaactcctga caatataatt acagcagaaa
    11761 ttaacagaag tgtcagatat ccgttgaagt ttttcttaat tattttaaag tgtttttgca
    11821 taaaatataa tggagttcaa cttttataaa atattttagt tccttttaat ttcctttaaa
    11881 attagatc
//
LOCUS       DAORVQ010000100        11758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2703_length_11758_cov_4.871742, whole genome shotgun sequence.
ACCESSION   DAORVQ010000100 DAORVQ010000000
VERSION     DAORVQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_11758_cov_4.871742"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..648
                     /locus_tag="PLX80_09140"
     CDS             220..648
                     /locus_tag="PLX80_09140"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQY20992.1"
                     /translation="MNKKILILCTGNSCRSQMAEGFLRDLDKDLGVYSAGTFPAEKVN
                     PNSIVVMNEIGIDISANKPNDVSDYVNENFDYVITVCDNAKEICPVFTGNVKHRLHLG
                     FIDPAEAKGTPEEILQEYRDVRDEIKSTFRHFYNTKLKDN"
     gene            716..1093
                     /locus_tag="PLX80_09145"
     CDS             716..1093
                     /locus_tag="PLX80_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HQY20993.1"
                     /translation="MLQIIYIGIGGFLGAILRFLTVKYFNNIFPTFPAGTLIVNAAGS
                     FLIGLLAFSFTDGKYLTPELRDLLIIGFIGAFTTMSGFAFESVRLFESANFFYFGLNL
                     FLNVFLSLIAVVAGRELILYIAK"
     gene            complement(1209..1889)
                     /locus_tag="PLX80_09150"
     CDS             complement(1209..1889)
                     /locus_tag="PLX80_09150"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HQY20994.1"
                     /translation="MTDNILDEVWGKIRLLLKSHPWHGVPIGKAVPKTVTTYIEIVPT
                     DTVKYEIEKHSGFLKVDRPQKYSNVCPTPYGFIPQTLCGDRVGEYCMKMTGRENIQGD
                     GDPLDICVITEKSISHSNILLQAIPIGGFRMIDHNEADDKIIAVMKDDAIFSEWKNIS
                     DCSHSFIERLKHYFLTYKDAPGSDGKHCEIHSVYGIEEAYEIIRASHQDYIEKYSDLN
                     VLLNREEI"
     gene            complement(1920..2531)
                     /gene="ruvA"
                     /locus_tag="PLX80_09155"
     CDS             complement(1920..2531)
                     /gene="ruvA"
                     /locus_tag="PLX80_09155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQY20995.1"
                     /translation="MISSLRGKLIYRNNNEVILEVSGVGYQVFVSKKLWESLIEMNSE
                     YLIFTHLDVKENSMQLYGFSDEKEKELFKMLLSVSGIGPKMAHNISAALTFEEILSLI
                     SDKTFSRSVKIPGIGAKKLELISMTLKDKIFKIDKETGKGVFEMPEEISGDNIRLEAL
                     NALLNLGYGRADGEKLIREALKKNETKINTTEELIRKALDLIS"
     gene            complement(2542..3102)
                     /gene="ruvC"
                     /locus_tag="PLX80_09160"
     CDS             complement(2542..3102)
                     /gene="ruvC"
                     /locus_tag="PLX80_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQY20996.1"
                     /translation="MRIIGIDPGTSVTGVGVIEVINGSFKMIYFGNITLNSKDPMPVR
                     LKKIYDECFDCIKKFEPEELAIETAFYSKNIQSTLKLGQARGVAMIAGLNSDVKISEY
                     SPKEIKKSVTGNGASSKEQVLYMVSSILSINKTHLPIDSSDALAIALCHSFNIQSNFL
                     PSNKKSGKNSWKTYLENNPERILTKS"
     gene            complement(3127..3855)
                     /locus_tag="PLX80_09165"
     CDS             complement(3127..3855)
                     /locus_tag="PLX80_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQY20997.1"
                     /translation="MSGHSKWATIKRKKAATDSARGRVFTKIIKEITIAARDGGGDPD
                     GNPRLRLAIQTAKANNMPQDNITRGIKKGTGELEGVRYEEITYEAYAPNGIAMIIESV
                     TDNKNRTVADLRHGISKAGGNLGESGSVAWMFERKGVVNVEKGDIKEDDLMEIILDAG
                     AEDLKSEGEYFEVVTPIENFETVRKAIEDKSFVIESASLQYIAKDLLPVEGKNADAVI
                     RCIEAVEDNDDVQNVFTNADFLDE"
     gene            complement(3895..5685)
                     /gene="recJ"
                     /locus_tag="PLX80_09170"
     CDS             complement(3895..5685)
                     /gene="recJ"
                     /locus_tag="PLX80_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQY20998.1"
                     /translation="MKKNWKLKTLSKHGISASELSNTDDPNIDDLIDDFRGLKSNIEM
                     PEVLIRLLYMRGITDYQKVLKFFKPTIDKLYDPALMKDAGIAADRIIKAINDKEDIMI
                     LGDYDVDGTCGVSMFYLFLKKFGLEPEYYIPDRIEEGYGISMKSINYAKEKKIALIVA
                     IDCGITAFEKVEYAKSLGIEFIICDHHQPPENIPNALAVMDPMRVDDDYPFKYLCGTG
                     VAFKLAQFIAQKLDKPESTHSLLDLVAIATASDIVPLNDENRILVSEGFKLINNSPRP
                     SLKLLIESSGLKAGNLNTGNVVFTVAPRINAVGRLGDAKRAVELLICEDDEKIKEFTS
                     VLNVENTNRRDIDKSITENAFKQFEETNEIDTLKSIVIHNEDWHSGVIGIVAARLVEK
                     YNLPSIVLTTVNGVAKGSARSINTFNIYEALKQCEDKLIQFGGHFHAAGLEVELSNLE
                     EFKKSFNSIATNQITDAELIPEIEIDAEISFEQLTDKFIKILDFFEPYGPENMTPVFV
                     TRDVQIIGDVRYAKSDTHIFKVRDRENKKIFEAVYFNSPNFRYEAIKDEDTAGKIVSG
                     NYCDICYSVEKNYWNNRETTKLRIRDMKFD"
     gene            complement(5708..5791)
                     /locus_tag="PLX80_09175"
     tRNA            complement(5708..5791)
                     /locus_tag="PLX80_09175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5755..5757),aa:Leu,seq:cag)
     assembly_gap    6010..6109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6411..7343)
                     /locus_tag="PLX80_09180"
     CDS             complement(6411..7343)
                     /locus_tag="PLX80_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY20999.1"
                     /translation="MDKNSESQETRPERSFRNTYRLEEGKSKNGLIIGFVTGLVLLTV
                     IIIFLFSGPSDYEKGLQFIHQKQYSEALVEFQKVEPGDKDFNMAQSKINYINGKNAFN
                     DGLYPQAQVYLSKVEMSDEFYQESQLMIDKTKVSERENDLTSLSEKINEVNKDTLIVI
                     EKDGETTDNINNNNDKSDEAVTDNSKNEKANMLYITSLEKLINNYKILFNNAKDAGDV
                     SKKETMRLMDSIYNETENLDYAVSEKNAMVMELKSLTGTWMQKSLTYLNSNNNENKVN
                     AENSAGTMDKNKEDYEKQYTLMINMLKKVKTYFS"
     gene            7685..8206
                     /locus_tag="PLX80_09185"
     CDS             7685..8206
                     /locus_tag="PLX80_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21000.1"
                     /translation="MESNNKHLPYFISGGLLLSFLFDIAGSFFPSESLAQTILFKIDT
                     LLAITAFACLGSKAASEKYDIASAGFGILAIAQGLFLAKIDQALHWNYDTADTAILFL
                     LPALVLISYYKPFPKWLRYGGILLIIPFLMILIIHLISSTTDLSFYVNIIFLLYHSVT
                     LCWAWQIWNKRNN"
     gene            8440..9705
                     /locus_tag="PLX80_09190"
     CDS             8440..9705
                     /locus_tag="PLX80_09190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY21001.1"
                     /translation="MKNIHTAFFALFLCIIFNQSELKAQDVPSNTNIPGIENFDNEFI
                     SFMEKWEVPGGQVTVCKDGRLVYSKAYGYSDLENKIPATTDELFRIASVSKPITAVAI
                     MKMIQEGKLKLSDKALDILSDFTPPPGTTVIDKRWYDITVENLLEHTGGWTLVNGDRQ
                     IIYLRKAADAFGTPRPADAKSIIRYGMKDSLEFTPGTEYSYSNFGYNILGRIIEKISG
                     KTYEKYMQEEILALAGISDMLIAKTRDIYRLPNEAKYYTENDNKDVFWSVLDEEEKMV
                     TMAYGADYFIEVMDSHGGWLSTAEDLAKFVTSVDTKTNRPHILTQATVDNMLSEPTLK
                     PKADATQYYAKGWNYEPYTDSWFHAGALAGVTSYIIRMGDGTTVTVIFNFLSMSKLGD
                     YLTELTKSTVPNSLKSVKQWPAEDLFNIK"
     gene            complement(9867..10934)
                     /locus_tag="PLX80_09195"
     CDS             complement(9867..10934)
                     /locus_tag="PLX80_09195"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQY21002.1"
                     /translation="MNIKENVKLSNHTAISLGGNAKYFAVCKSIDEILEALEFAQNNK
                     LRIQLMSGGSNIIFTDNGFDGMIIKNDIKGISEKVNEDHVLISVGAGEELDEFVKYCV
                     GKGYSDIECLSGIPGSVGAMPVQNVGAYGQETSEVLESIKVIDRNETDIKNIKTFSNE
                     ECQFGYRMSRFKKSDKDRYIIVEVNLKLHINKLPEIKYKALGERLDQNDSFKNSDDIK
                     SKLKIIRDTVISIRKEKSMVIDKNDINSRSCGSFYMNPVLNEDCFSKFSKVCSELQTE
                     FPFYKSGNDYKIPAAWLVENSGFPKGYKKGGVGISQNHTLALINIDGTAKELLDLSDE
                     IKSSVYNKFRIKLFTEPEIIS"
     gene            11219..>11758
                     /locus_tag="PLX80_09200"
     CDS             11219..>11758
                     /locus_tag="PLX80_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HQY21003.1"
                     /translation="MLKSIKSKTQFVSADDAVKCIKSGDNVYVHANCSFPLTLINSLC
                     SRYEELKNVNIIQLMSFLDAPYVRPEMEGHFNLISLFTGANVRKAVHEGRADFIPVFL
                     SEIPILLKNKKLPVDVCLLHLSPPDTHGFCSLGVSNECTKTAAENSRIIIAQINPKMP
                     RVLGDNFIHIDKIDFAVEVN"
BASE COUNT         3529 a         2208 c         1811 g         4110 t
ORIGIN      
        1 tgtatgaccc gtttcatttt caaaatgatc ttttttattc catttttatt tttatttaaa
       61 agggatcaat tctgattcac ttacctttaa tttaaaaaag cttcctgtag tatttttcat
      121 tacaggaagc ttctttttta ttaaaattaa cacaaagtta ttctaaacaa aaatttattt
      181 ttactatatt atattcgttt caaataaata aaaatcattt tgaataaaaa aatactgatc
      241 ctctgcaccg gaaactcctg cagaagccag atggcagaag gctttctcag agatcttgac
      301 aaagatctcg gtgtatattc agccggtact tttcccgccg aaaaagtaaa tccaaattct
      361 atagtagtta tgaatgaaat agggattgat atatccgcta ataaaccaaa tgatgtatcg
      421 gattatgtaa atgaaaattt tgattatgtg attactgtct gtgataatgc aaaggaaatt
      481 tgtcctgtat ttaccggcaa tgtaaaacac agactgcatc ttgggtttat tgatccggct
      541 gaagctaaag gaacccctga agaaatacta caagaatatc gtgatgtcag agatgaaata
      601 aaaagtactt tcagacattt ctataataca aagctcaaag ataattgagc aataaaataa
      661 actataaata acttatctta ttctgtattt tcagaactaa tttaatttat aatatttgct
      721 tcagataatt tatataggaa tcggaggttt tctcggagct atacttagat ttcttacagt
      781 aaaatatttc aataacattt tccccacttt tccggcaggt acactcattg taaatgcagc
      841 cggaagtttc ctcatcggtc ttcttgcatt cagttttact gacggcaaat atcttacacc
      901 tgaactgcgg gatctgctaa taatcggatt catcggagca ttcaccacaa tgtctggatt
      961 tgcatttgaa tcagtcagac tattcgaatc agcaaacttt ttttattttg gattaaacct
     1021 gtttctgaat gtgtttcttt cattaattgc agtagtcgca ggacgcgaac ttattttgta
     1081 tatagcgaaa tagttttcat atataaatct tcaattactc tttttaaatt aataaatttg
     1141 caaaatataa tttatctgca ttcgcacttc aattaaatta ttttttattt aaaatttaaa
     1201 attactcttc agatttcttc tctgttcaga agcacattca gatcactgta tttttctata
     1261 taatcctgat gactggctct gattatttca taagcttctt caattccata cacactgtga
     1321 atttcgcaat gctttccatc tgatccggga gcatctttat aagtaagaaa ataatgttta
     1381 agtctttcga taaatgaatg tgagcagtcg ctgatatttt tccactcact gaatatagca
     1441 tcatctttca tcacagctat aattttatca tcagcctcat tgtgatctat cattctgaat
     1501 ccgccgattg gaattgcctg caaaagtata ttactgtgac tgatcgactt ttctgtgata
     1561 acacatatat cgagcggatc accgtctccc tgaatatttt cacgtccggt cattttcata
     1621 cagtactccc ccaccctgtc tccgcaaaga gtctgcggaa taaatccata cggggtggga
     1681 catacattgg aatatttctg cggtctgtcc actttgagaa atccactgtg cttttcgatt
     1741 tcatatttca cagtatcagt cggcactatc tcaatgtaag tcgtgactgt tttagggacg
     1801 gctttcccga tcgggacacc gtgccatgga tgtgatttca gtagaagtct tatcttgccc
     1861 cagacttcgt caagtatatt atctgtcatt ataatttaaa aaatattaat aattaatttt
     1921 tatgaaatta gatccagagc ttttctgata agctcttcag tagtatttat tttcgtttca
     1981 ttttttttca aagcttcacg tataagtttc tctccgtcag ctcttccgta gccaagattt
     2041 aaaagtgcat tcaaagcttc cagtctgata ttgtcaccgg aaatttcctc aggcatttca
     2101 aatacacctt tacctgtttc cttgtcgatc ttaaaaattt tatccttcaa agtcattgat
     2161 atcagttcga gtttctttgc acctatgccc ggtattttta cagatcttga gaatgtctta
     2221 tctgaaataa gtgaaagtat ctcttcaaaa gtaagtgccg ctgaaatatt atgagccatc
     2281 ttcggaccga taccgcttac agacaaaagc atcttaaata attctttttc cttttcatct
     2341 gaaaatccgt agagctgcat cgaattttct tttacatcga gatgtgtaaa gatcagatat
     2401 tccgaattca tttcaattaa tgactcccat agtttttttg acacaaacac ctgatatccg
     2461 acacctgata cttctaatat cacctcatta ttatttcgat agataagttt acctctgaga
     2521 gatgagatca ttttaaatta attaggattt tgttaaaatt ctttcaggat tattttcgag
     2581 atatgtcttc cagctgttct taccggattt tttatttgac ggtaaaaaat ttgactgaat
     2641 attaaaggaa tgacaaagtg ctattgcaag tgcatctgaa gagtctatag gcagatgagt
     2701 tttattaatg ctcagtatcg aacttaccat ataaagtacc tgctcttttg atgaagctcc
     2761 gtttcctgta actgattttt tgatttcctt cggagaatac tctgaaattt tcacatccga
     2821 attaagtcct gcaatcatcg caactcccct tgcctgtccc agcttaagag tcgactgaat
     2881 atttttactg taaaatgctg tctcgattgc aagctcttca ggttcgaatt tttttataca
     2941 atcaaaacat tcatcataga tcttttttaa tcttaccggc atcggatctt tggagttcaa
     3001 agtgatattt ccaaaataga tcattttaaa ggacccgttg atcacttcta tgacacctac
     3061 tccagttact gatgtacccg gatctattcc gatgattctc attgataaat tttattcgga
     3121 aatttattac tcatctaaaa aatcagcgtt ggtaaaaaca ttctgcacat catcattatc
     3181 ttccactgct tctatacatc ttatcacagc gtcagcattt tttccctcta caggcaaaag
     3241 gtcttttgca atatactgta gtgaagcact ttctattaca aaagatttat cctcaatagc
     3301 ttttctgact gtttcaaaat tttcgatcgg agttacaacc tcaaaatatt caccttccga
     3361 tttcagatct tcagcacctg cgtcaagtat tatttccatc agatcatctt ctttgatatc
     3421 acccttctca acattcacaa ctcctttcct ttcaaacatc caggcgaccg aacctgactc
     3481 tccgagattt ccgccggctt ttgaaatgcc gtgtctaaga tcagcaactg ttctgttttt
     3541 attatcggtc acactttcga tgatcattgc aatgccgttt ggagcataag cttcataagt
     3601 aatttcctca tatctcacac cttcgagttc gcccgttcct tttttgatgc ctcttgtaat
     3661 attatcctgc ggcatgttgt ttgcctttgc agtctgaatg gcaagtctca gacgcggatt
     3721 accgtccgga tctccgccgc catctcgcgc tgctattgtt atctctttaa tgatctttgt
     3781 aaatactctt cctcttgcgg aatcagtggc ggcttttttt ctttttatag tcgcccattt
     3841 ggaatgacct gacattggtt tttactccgt aaagttttta ttttttaaat aattttaatc
     3901 aaatttcata tcgcgaattc gcagctttgt ggtctcacgg ttattccagt aatttttttc
     3961 gactgaataa caaatatcgc aatagttacc cgaaacaatt ttccccgcag tgtcttcatc
     4021 cttaatagct tcatatctga aattaggtga attaaaatat actgcttcaa atattttctt
     4081 attctctctg tcacgaactt taaaaatatg cgtgtcagac tttgcatatc ttacatcgcc
     4141 tattatctga acatctctcg tcacaaatac cggcgtcata ttttccggac catatggttc
     4201 aaaaaaatca agtatcttta taaacttatc tgtaagctgt tcaaaagaaa tctcagcgtc
     4261 gatttcaatt tcagggatca gctcagcatc agtgatctga tttgtcgcaa tgctgttaaa
     4321 ggatttctta aattcctcaa gattcgacag ctctacctct agacctgctg catgaaaatg
     4381 cccgccgaat tgtatcagct tatcctcaca ctgcttaagt gcttcatata tattaaatgt
     4441 attaatactt cttgcactgc cttttgcgac accgtttaca gtggtcagaa caattgacgg
     4501 cagattatat ttttctacaa gcctcgcagc gacaattcca atgacacctg aatgccagtc
     4561 ttcattatga atcacaattg attttaaagt atcgatttca tttgtttctt cgaactgctt
     4621 aaatgcattt tcagtgatcg atttatcaat gtcacgtctg ttagtatttt ctacattcag
     4681 cacagatgta aattctttaa tcttctcatc atcttcacat atcagaagtt cgactgctct
     4741 ttttgcatca ccgagtcttc cgacagcatt gatcctcgga gctacagtaa acactacatt
     4801 tcccgtattc agattaccgg ctttaagacc gctggattct atgagcagtt tcagagaagg
     4861 tctcggacta ttattaatga gcttaaagcc ttcgcttacg agaattctgt tttcgtcatt
     4921 aagcgggaca atatcggaag cagttgcaat cgctacaagg tctaacaatg aatgtgtaga
     4981 ttcaggtttg tcaagttttt gggcaatgaa ctgagcgagc ttaaaagcga cgcctgtacc
     5041 gcagagatat ttgaacgggt aatcatcatc cacgcgcata ggatccatca cggccagcgc
     5101 atttggaata ttctccggcg gctggtggtg atcgcagatt atgaattcta tgccgagaga
     5161 ttttgcatac tcgacctttt caaatgcagt aataccgcag tcgatcgcaa ctattaaagc
     5221 aatttttttc tctttggcat aattgatcga cttcatggaa atgccataac cttcttctat
     5281 cctgtcggga atatagtatt caggctcgag tccgaatttt ttgagaaaga gataaaacat
     5341 cgacacgccg caagtaccat ccacgtcgta atctccgagt atcataatat cctctttgtc
     5401 attgatagcc ttaatgatcc tgtcggcggc aatgccggca tccttcatta aagccgggtc
     5461 gtaaagcttg tctatggtgg gtttgaagaa tttcaggact ttctgataat ccgtaattcc
     5521 tctcatgtaa agcagccgga tcagtacttc cggcatttcg atgttacttt ttaatcctct
     5581 gaaatcatct attaaatcat ctatatttgg atcgtctgta ttactcagtt cagacgcaga
     5641 gattccatgc ttgctcagcg tttttagttt ccaatttttt ttcaattttc atctatttta
     5701 tttaatgtgc tcaggggggg actcgaaccc ccaccttaat tctaagactg caccctgaat
     5761 gcagcgcgtc taccagttcc gccacctgag cttttatcgt gacagcggga agataattat
     5821 ataggtttgt aattacaatt tgaaattttt tcttattttg ggaagtatga tctttctcat
     5881 catcagcatg aaagaaatac agtttttgcc ataaaggctc gaagtctcga aggattaaag
     5941 actttaaaca aaaatattaa tatataaata gaaattaact tagggacttt gaggctattc
     6001 aaaaaaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng attggtttgc
     6121 agaattgtgt ttaatttgac tccacggttt actttgctga gtttataaat ttgatgcttg
     6181 ctatttcttt ctgcaaaaaa cccttcactc ccctttttta gtgactgtct caaaacatct
     6241 gttcttacca ataagacacc gagacacaaa ggaaaaaagt taaaaaatat taatctttgt
     6301 gacttagtga ctttgtggtg aaattattta actgttatta gacagactct ttaaagaaac
     6361 gtatttcgct ctttcaggaa catcaataat ggctctgaaa taaaatacta ttatgaaaag
     6421 taagttttta cttttttaag catattaatc atcagtgtat attgtttttc ataatcttct
     6481 ttattcttat ccattgtacc agcagaattc tcagcattta ctttattttc attattatta
     6541 gagtttaaat aagtcaaaga tttctgcatc caggtccccg tgagactttt cagctccatg
     6601 accattgcat ttttctcact cactgcatag tcaagatttt cagtttcgtt gtatattgaa
     6661 tccattaatc tcattgtttc cttttttgaa acatcaccgg catcttttgc attattaaat
     6721 aatatcttat aattatttat taatttttca agagatgtaa tataaagcat gttagctttt
     6781 tcatttttag aattgtcagt tactgcctca tctgatttat cattattatt gttaatatta
     6841 tcagttgttt ctccatcttt ctctattaca attaaagtat ccttatttac ttcatttatt
     6901 ttttcactca atgaagtaag atcattttca cgttccgaaa ctttcgtttt atcaatcatt
     6961 agttgagact cctgataaaa ttcatccgac atttccacct ttgataaata tacctgagct
     7021 tgagggtata aaccatcatt aaaagcattt tttccattta tgtaattaat tttagattga
     7081 gccatattaa aatctttatc tccgggttca actttctgaa actccaccaa tgcctctgag
     7141 tattgttttt gatgtataaa ttgcaatccc ttttcataat cagatggacc cgaaaataaa
     7201 aatattatta ttacggtcaa tagaacaagt cctgttacaa atccgattat taaaccattt
     7261 ttactttttc cttcttcaag tcgatatgta tttcgaaaag atctttcggg tctggtttcc
     7321 tgtgattctg aattcttatc cattttatta tttaatttta tattgattga aaaaattaac
     7381 ttctgttata tgcaacatat tcaattttcg tgccaatttg attcaataac aaaaaagcag
     7441 ttctgagaaa attaaattgt cattaatgtc aaaagtgtca ttctaaagac agaatttttg
     7501 gatagagaag caacatcaaa gccacatgct gttcgtgctt tcgctcaatg tagctactat
     7561 tacttattgt atttttaaaa aatttttcat agtcgataca aagttaaaag ttaagaatta
     7621 atattataat atttaattct gactattttt agtaaattct ttcattaaaa attcaattta
     7681 cctattggaa agtaacaata aacatctccc gtattttatt tcaggtggat tattactgag
     7741 ttttcttttt gacatagccg gttcattttt cccttctgaa agtcttgccc agacaatact
     7801 tttcaagatc gacactttat tagccattac tgcttttgcc tgtctcggct caaaagccgc
     7861 ttctgagaaa tatgatatcg catctgcggg attcgggata ctggcaatag cacagggatt
     7921 atttcttgca aagattgatc aggcattaca ctggaattat gacacagcag atacggctat
     7981 acttttttta ttaccggcat tggtgcttat ctcatattat aaaccttttc cgaagtggct
     8041 taggtatggc ggcattttat tgatcatccc gtttcttatg atattaataa ttcatttaat
     8101 ttccagcact accgatttat ctttttatgt aaacataata tttctgctat accattctgt
     8161 aacactttgc tgggcatggc agatctggaa taagcggaat aattgatcca ggcttttgct
     8221 ggtataaatc ctttacctcc atgcctctaa gcctctaaga ctataagact ctaagactct
     8281 aagattaaaa atttaagaac attaatatca aaattctttg aaccttcgag tctcagaggt
     8341 atccaaagtt ttaagataga ttatgagagt gttaacgagc ttattattta ctgtttatat
     8401 aatttcataa ttcatatttt ctgtatatta aattttaaaa tgaaaaatat ccatactgct
     8461 ttcttcgcac ttttcctttg catcattttt aatcaatctg aattaaaagc acaggacgtt
     8521 ccttcaaata ctaatattcc gggaatagaa aattttgata atgaatttat ttcctttatg
     8581 gaaaaatggg aggttcccgg aggacaggtt acagtctgca aagacggaag actcgtttat
     8641 tcaaaagcct acggatacag cgatctcgaa aataaaatac cggcaactac agatgagctc
     8701 ttcagaattg ccagtgtctc aaagccgatc accgctgttg caattatgaa aatgatacag
     8761 gaaggaaaat tgaaactcag cgataaagcg ttagacatac taagtgattt tactcccccg
     8821 ccgggaacta ctgtaattga taagagatgg tatgacatta ctgttgaaaa tttactggaa
     8881 cataccggtg gatggacttt agtaaatggc gacagacaga taatatatct ccgtaaagct
     8941 gccgatgctt ttggaacacc aagaccggct gatgcaaagt caattatcag atatggaatg
     9001 aaagattcat tagaattcac accgggtacc gaatatagtt actcgaattt cggatataat
     9061 attctcggca ggatcataga aaaaatatca ggaaaaacat atgaaaaata tatgcaggaa
     9121 gaaatacttg cccttgcagg gatctccgat atgctgatcg caaagacacg tgacatttac
     9181 agacttccga atgaggcaaa atattatacc gaaaatgaca acaaagatgt tttctggtca
     9241 gtattggatg aggaagagaa aatggtgacg atggcttacg gtgcagatta ttttattgaa
     9301 gtgatggatt cgcatggcgg atggctttcc actgcggaag acctcgcgaa gtttgtgact
     9361 tcagtcgata ccaaaacaaa cagacctcac attcttactc aggctacagt agataatatg
     9421 ctttctgaac caaccttaaa accaaaagcc gatgcaactc agtactatgc taaaggctgg
     9481 aattatgaac cttacacaga cagctggttt catgcaggtg cgctggcagg tgttacttct
     9541 tatatcataa gaatgggtga cggaacgact gtcacagtga tatttaattt tctttcgatg
     9601 tcaaaactcg gagactatct gacagaactt acaaaatcta cagttccaaa ttctttaaag
     9661 tctgtaaaac aatggccggc tgaagacctt tttaatatta aataataaat ttttaataat
     9721 aaataatttc aacatttcca aatctgcaaa acgtacgata actttggatt gcctcaaagg
     9781 ctataagact caaagataat aaatattttt ataaattcct ttgagccttc gagtctttga
     9841 ggcaagctta aaaattttgt tttcgattaa gaaataatct caggctctgt aaataattta
     9901 atccgaaatt tattatatac tgaactttta atttcatcag aaagatctaa aagctccttt
     9961 gcagtcccgt caatatttat caaagcaagt gtatgatttt gcgaaattcc gactccccct
    10021 tttttatatc cttttggaaa gcctgaattc tctaccagcc aggcagctgg tattttataa
    10081 tcgttaccac ttttgtaaaa tggaaattca gtttgcaatt cactgcagac ttttgaaaat
    10141 ttactgaaac aatcttcatt caaaacagga ttcatataaa atgatccgca tgatctggaa
    10201 ttaatatcat ttttatctat caccattgac ttttcttttc tgatggagat cacagtgtct
    10261 ctgatgattt tcaatttact ttttatatca tctgaatttt taaatgaatc attttgatct
    10321 aacctttcac cgagtgcttt atattttatc tcaggtaatt tattgatatg aagttttaaa
    10381 tttacttcga ctatgatata tctgtcctta tctgattttt taaatctgct cattctgtat
    10441 ccaaactgac attcttcatt tgaaaatgtt tttatgtttt ttatatcagt ttcatttctg
    10501 tcaatcactt ttatactttc aagtacttca cttgtctcct gcccgtatgc gccgacattc
    10561 tgaacgggca ttgcgccgac tgagccgggt attcctgaca gacattcaat atctgaataa
    10621 cctttcccaa cacaatattt aacaaattca tcaagctcct cccctgctcc tacagatatt
    10681 aaaacatgat cttcatttac tttctcagaa attcctttga tgtcattttt tataatcatg
    10741 ccgtcaaatc cattatcagt aaatattata ttgcttcctc cagacatcag ctgaatccgc
    10801 aatttattat tttgagcaaa ttccaatgct tctaatatct catcaatgct tttacaaaca
    10861 gcaaaatatt ttgcattacc gcctaaagaa attgcagtat gattcgaaag ttttacgttc
    10921 tcttttatat tcaaattatt tagatcaaaa attttttcaa taatataaat ttaatgataa
    10981 gttttagaaa cagattaatt taaatcaatt gttggatgtg agtttgaaat tgccgcaaag
    11041 gctctgagtc tcaaagattt taatataaaa actttaatat taaatttcat tgaggctttg
    11101 agactttgag gcaaactaaa aaatttcaaa atatgatttt tatcataata ccttatattg
    11161 tctttttaat ataatataat gagagtaaat tatactcaca aatttcaaac cctaaataat
    11221 gctaaaatcg atcaaatcca aaactcaatt tgtctcagct gatgatgctg taaaatgtat
    11281 taaatccgga gacaatgtat atgttcacgc aaattgttct tttcctctta cattgataaa
    11341 tagtctatgc agcagatatg aggaactaaa aaatgtaaat attattcagc ttatgtcatt
    11401 ccttgatgct ccttacgtga gacctgaaat ggaaggacat tttaatctta tatcattatt
    11461 tacaggagca aatgtaagaa aagcagtgca cgaaggtaga gctgatttca tacctgtctt
    11521 tctttctgag attccgatat tactgaagaa taaaaaactt cccgtggatg tatgtctgtt
    11581 gcatttgtct ccgcctgata ctcatggctt ttgctcacta ggtgtaagta atgaatgtac
    11641 aaaaactgct gcagaaaatt ccagaataat tattgctcag ataaatccaa aaatgccgag
    11701 agtactcggt gataatttta ttcatattga taaaattgat tttgcggtgg aagtaaat
//
LOCUS       DAORVQ010000101        11603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2766_length_11603_cov_5.394008, whole genome shotgun sequence.
ACCESSION   DAORVQ010000101 DAORVQ010000000
VERSION     DAORVQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_11603_cov_5.394008"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLX80_09205"
     CDS             complement(<1..339)
                     /locus_tag="PLX80_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21004.1"
                     /translation="MNTKFTILLLLLVSISLTVSGYSGNNENTGNKKSSDLAPYNTAS
                     ENLLFKKKEKAIEGPELEKYYFEEWHYPYGSLLPKEIIDKIWAETKAVPEEEDSGDAA
                     VNSWRTIGPYG"
     gene            499..1956
                     /locus_tag="PLX80_09210"
     CDS             499..1956
                     /locus_tag="PLX80_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21005.1"
                     /translation="MHKSKLILLLKTFSGEENIRFHDFLSSIYFNKNKTLIKFFEILR
                     KEYPDFDSDKILKKNVYLKLFPENEFNEQVLKNLISDLLKLCREFLEIEIYEKDKNEK
                     QLNLLKQLTGRNADPVYFTELKNFEKQLNELSELSEKSFYYLYQLEELKISFHLERNE
                     QPKVIEKVMKSGEFLLLYFHLHLTKIISNLNVNKHTYKVDYKTNLPGEFFDNINFEKV
                     IKYMKEKDISYSEIVEMYYLRVICNSQQFNEKNYFKFKELVIKNLNLLDRVETYGLFN
                     ALETFNMKYYSSSNEFFMNELLDIYKTEIENGFYKFSDTSPVTLMKFRNTFNSAVRSG
                     EFEWAENFLDDFQNDVIESDRNNLIKISHAQINFETGDFEKSLELISEINPDHLYLKI
                     DIRNLTLMSYYELNYLDSAISAIDSFKHFISSNKSLSDHFKESNIRFANSLNSLILFK
                     EKDQKEKLADLKIDLIPFKGERRINWLMEKIDEVI"
     gene            2706..5144
                     /locus_tag="PLX80_09215"
     CDS             2706..5144
                     /locus_tag="PLX80_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY21006.1"
                     /translation="MKKKKLIYMLLLIFIGVLNFENSNAQMFWNKSCYFEGNSNSYIS
                     TEDSPNLNITGSFTIEAWINPEVAGVTQTLIRKREGLDNSGYSMYLFNGRVAIRTNSI
                     TRLVGKTVIPANIWTHINVTYNNFTNEFYMSINGLVDTSTIKAGAAPLTNTDSLFIGN
                     GNDGPFNGILDEVRIWKESRSSGEVASLMRTSLGTSSGVYSKLVLSLTFQNRHSDGTM
                     GNVFSLKDWSGNSNIFYNRGIFNHNLSNRPSTTIFTNLSANLDGNNDYLAAADESSFS
                     PTAVFTLEAYVYPKSFSIGEVIIHKGLDNGLVTNYGLRLNQGNVVAYINNQVNLISQN
                     SIPLNKWTHLAYVYNTIFGTNLLYVNGVLTDSASSNTGLVTNGNDSFYVGGTIALPDF
                     NGYIDEVRIKSAIKNYSEINQFLYKSIDESNDLNGTELVFNLDGGTLSNVGLAIDLKF
                     RNDAEFGNPGTSYDIPVSPLHRADNIDFNEGFILKNSDRRIPGAGTSGNMTDDSLYVY
                     QNDIINDINVFVALNHKNENSLSITLVSPNGTSIDLYNNNNNFLGYNNSIVTVFDDES
                     NYLNSNNTYVSFEPSIKPQSNINSAFSGLSSFGYWKLLVNDNSPGDTGRLVSWGIQIN
                     ESVNKNFNLNSTSIIQGFYNPLTNTSVRDTMQIKLRKSTSPYIVIDSAKSFYLPSGFS
                     TYTFKNAVAGTPYYLQLDHRNSIDTWSSNFITFEALSGQSNYNFTFNSAQAFGNNLMQ
                     ADTSPLRFAIYNGDVNKDGIVDVTDNSLVDNDAFNFNTGYIVTDLTGDNTVDLSDATI
                     VDNNASMFISKITP"
     gene            5547..6143
                     /locus_tag="PLX80_09220"
     CDS             5547..6143
                     /locus_tag="PLX80_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQY21007.1"
                     /translation="MKRTKQEAEQTKNELLDVAFKEFLETGYNECRLEDIAAKAGVTR
                     GALYWHFKNKDDLLDSLISQKDIESNKIADEIAGSDDEPFEKLKKFVNLNFPDLSGGR
                     KEKNYVKLKVELYKHFNEKGDKNKSAEKFTAHCKKFIEQCKSRGEIKDEIDPADAANT
                     IMIICAGSYIRFNSIQSNKRAIKYSKKLALDYLYLIHK"
     gene            6148..7557
                     /locus_tag="PLX80_09225"
     CDS             6148..7557
                     /locus_tag="PLX80_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQY21008.1"
                     /translation="MKKLILLLFIFAIEIFLSVGLKAANFDSTFAEQLQQRINLLKTN
                     NNLVGISAAAYVPGQGMWTGSYGISSSDSDEITTEMVLNSGSVAKNFMATIILMLAEA
                     DSLNLSDTLGQWLSPYNNIPGNVTIKQLLDHSSGIYNVTDNPAFISAINSNLNRLWTI
                     EEVLKGGYILSPYFLPGAAFRYSNTNYMILGLIIKKIMKTTLAEQFKERFFKPFSLNE
                     TYFEFDDTVKSPFAHNWADFTGSGVRQDAFHIPTTSINSSTIGAGGVVSRPENLARWL
                     IALFEGHILNANSMTQMLTFRNVSIAGGNGYGLGVIRYNVPGNVCYGHGGNIFGYSTV
                     AIYDPVDSISIAVMINNDVNAGPIGINFMSFVKQNNPVSISEISDAIPEKYTLEQNYP
                     NPFNPVTNINFSIPHRSFVKITVFDILGKEIQNLINEELNEGNFKIDFNGSSLPSGIY
                     FYKIETNDFVQVRKMTLLK"
     gene            complement(7955..10063)
                     /locus_tag="PLX80_09230"
     CDS             complement(7955..10063)
                     /locus_tag="PLX80_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21009.1"
                     /translation="MLSKLINLFFILLISSFAYSQNKGLQPKEKSAEPRIEFNRNQDL
                     TDNRNSKEITDLESKLLLAKQSSNIDGTMEIVKELDALTGSLTKPGEEFPLHLIKSEN
                     TQSDNISIGLVSPVTGTKGVASCTEQIGSTASRIWTAFVFGPQSGTTTDALRLCFSDD
                     GGKTWIEKVTVGFSAGNRMWQDQIDIELIENSTGDKFIWVAFGYATNNYSGQYRIGVT
                     IVKITGLLNYSGYTLAWPGMVNSNYYWKPRIVSDNEAYRTNPWVYITACLDSAVAGGY
                     YSGEKVAICYSPYTVFPTFTYKPNGFLGLLFRYPVDYYVDIAYFKNGGQDSILIVESS
                     LEDSSKIVLAKTSISSFISSSFATYVGNISTIPDKRGYQAYIASAGGYNNLMIVNMRK
                     YSATDWDIEYYRSTNGSAGWINGYVDYRGNNSTRADIIGFRSAPGFYSCAYSENTQSI
                     IPVTYCSAENNIWVGLVFQMNHVDTNPFSAQPRPGIKYGPDGESCFAVWTEYSGGTNV
                     WASLGCSGTPRTYKYLSFNGLIEGFYNSASNIMNISDTVRLYLRSISSPYNILDSSKA
                     TIGNGGNGFFEFYNTDNLVNYYLQVKHRNSLETWSSSTFQFTNQYSSYQFSLDVTKAY
                     GDNMLQVDNMPVRFAFYSGDVDQDGIVDLTDMSLIFNDGLIFASGYLPTDLNGDSVVD
                     VGDAVFADNNGFNFVSKITP"
     gene            10256..>11603
                     /locus_tag="PLX80_09235"
     CDS             10256..>11603
                     /locus_tag="PLX80_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21010.1"
                     /translation="MTFQLYNNMLNSTLLDILRTFDKDQIKKFGIFLISPYHNNNSNV
                     IKLFNSLKTYSPEFINAGLEREILWKALFPGRDFHYGVMKNLIYELQKLTEKFLSFEK
                     YSSNEFEFEMNLLDALLDKKLITLFTKNAKNLKVQMLKSNIDLNYFYNKYILESRELN
                     YLYLNKNAANKPGSSTGNPNESLLIFFFINFFNSNYNSLHESILYNNPYDTEYVNSVT
                     GYFNISPVKKNKYASMFSSVIDTLLNPNDESIYFGLKNLFGKNSGRLGKELRYNVLTA
                     LINFCNFQIMKGNTKFIKEQFTIYKMMIESGNYNNGKDNYINPYMYINIVSMAANLRQ
                     YGWAEKFVERFKTKLETPHLDQCFNFAMVSLNLKRKKFDTAMEYLSRLKSGDVIDKIT
                     IRRFQLMLYYESGYFDELHSLIDSSRHFITNDHKTSARAKSIFKNFSSITISTLFLL"
BASE COUNT         3968 a         1862 c         1876 g         3897 t
ORIGIN      
        1 tccgtaagga ccgatcgtcc tccatgaatt tacagctgcg tctcctgaat cttcttcttc
       61 cggaacggct ttagtctctg cccagatctt gtcaatgatt tcctttggca ataatgagcc
      121 atatggataa tgccattctt caaaataata tttttcaagt tcaggtcctt cgattgcttt
      181 ttcctttttt ttaaaaagta aattttctga ggcagtgtta taaggtgcca gatcagaaga
      241 ttttttatta ccagtatttt cattattgcc ggagtatcct gataccgtta atgaaatact
      301 gacaagtaat aaaagtaaga ttgtaaattt agtattcatt ttagttagtt tggttttttt
      361 acaaaattaa caactattaa gtaaccatgc aaagaattat atttgaatta gtaatcacta
      421 tgagttaaat gaaatttcta aattgtttgt ttttaaactc aaatcaaatc ttttattatt
      481 aaaaagtaat ctgtaaaaat gcataaaagt aagctgattt tgttattaaa aactttttca
      541 ggtgaggaaa atatcagatt tcatgatttt ttatcttcca tctattttaa taaaaataaa
      601 actttaataa aattttttga aatactcaga aaagaatatc ctgattttga ttcggataaa
      661 attctaaaga agaatgttta tctgaaatta tttcctgaaa atgaatttaa tgaacaggta
      721 ttaaaaaatt taataagtga tttattaaaa ctgtgcaggg aatttttaga gatcgaaatt
      781 tatgaaaaag ataaaaatga aaagcagttg aatcttctga agcaacttac aggaaggaat
      841 gctgatccgg tttatttcac tgaactaaaa aatttcgaga aacagcttaa tgaactttcc
      901 gagctttctg aaaaaagttt ttattatctc tatcagcttg aggaattaaa aataagtttt
      961 catcttgaaa gaaatgaaca gccaaaggtt atagaaaagg ttatgaaatc cggtgaattt
     1021 cttttgcttt attttcatct gcatcttacc aaaataattt caaatcttaa tgtcaacaaa
     1081 cacacttaca aagtagatta caaaactaat cttccgggtg agttttttga taacattaat
     1141 tttgaaaagg taataaagta tatgaaagaa aaagatataa gctattcgga aatagtagag
     1201 atgtattatc ttagagtaat ttgcaattca caacaattta atgaaaaaaa ttatttcaaa
     1261 ttcaaagagc ttgttatcaa aaatctgaat ctgcttgaca gagtggaaac atatggattg
     1321 tttaatgctt tagagacttt taatatgaaa tattattcat catcgaatga attttttatg
     1381 aatgaacttt tagatattta taaaaccgaa atagaaaatg gattttacaa attttctgat
     1441 acttcacctg tgacattgat gaagtttaga aatacattta actccgccgt acggtcagga
     1501 gaatttgaat gggctgagaa ttttttagat gattttcaaa atgatgtaat agaatctgac
     1561 agaaacaatc ttataaaaat ttctcatgct cagatcaatt ttgaaacagg ggattttgag
     1621 aaatctcttg agcttatatc tgaaataaat cccgatcatt tatatcttaa gatcgatatt
     1681 cggaatctga cattaatgtc ttattatgaa ttgaattatt tagattcagc aatatctgca
     1741 attgattcat tcaaacattt tatatcttca aacaaatctt tatctgatca tttcaaagaa
     1801 agtaatatca gatttgccaa ttctctaaac tcactgattc tcttcaagga aaaagatcag
     1861 aaagaaaaac ttgcagatct gaagatcgat ctcattccat tcaaaggcga aagaaggatt
     1921 aactggctta tggaaaagat tgatgaagta atatagaatt aagttattat ttttcaggat
     1981 aatacttctt aaaacatttc tccttttttc gcatttggac acccaggctt tgaatagaat
     2041 tctataagaa tttgtatgaa tatttgtccg aacccgaagg gggtcttatg tttatgaaaa
     2101 atgttccatt atttttccaa ccccgaagta atacgtttta atctcaaaaa aacatcttaa
     2161 attcaaaatc taaatttttt cattcaaaca aattatactt tttattttct aatctattat
     2221 ctaatctatt ttctttaaga aaattcatgc atcgttttaa acatttgact ccttcgggga
     2281 tctctcattt aattcacata tattttctat aaatttcaaa cataactgat gacttgatat
     2341 tatttttcag tcagcctcat aaaattctaa gcttattaat ttagaatttt ctttaaaata
     2401 cctttgataa attaatctaa aaaaaatctg atcaaaaacc tgcttaataa atattcagtc
     2461 tgagaagatt gataatttat ttatttcaaa ttcaaagttt tatcatcaca tcatcattac
     2521 tttccatttt ttgaatgtct catatttaac ctgttttcta ataaagtcct taggtttcaa
     2581 atatactttt ctctaatatg acattacttt ttcaaatatt attctttgtc agaaaaatta
     2641 gtaattcata attttgtaca agttatttaa aataaattta aaaaaactaa aaactaataa
     2701 acaaaatgaa aaaaaagaaa ctaatttaca tgctgttatt aatattcatc ggggtattaa
     2761 attttgaaaa ttccaatgct cagatgtttt ggaataaatc ctgctatttt gaaggaaatt
     2821 caaacagtta cataagtact gaggattctc caaatctgaa tattactgga agttttacaa
     2881 tcgaagcatg gatcaatcct gaagttgccg gagttacaca aactttaatc cgaaaaagag
     2941 aaggacttga taattcggga tactcaatgt atttatttaa cggaagagtt gctatcagaa
     3001 caaactctat tacccgcctt gtaggtaaga ctgttatccc tgcaaatata tggacgcata
     3061 taaatgtcac ttacaataat tttactaatg aattttatat gtcgattaac ggtctggtag
     3121 atacttcaac tatcaaggca ggagctgcgc ctttgactaa tacagattca ctttttattg
     3181 gaaatggaaa tgatggtcca tttaatggaa tattggatga agtcagaata tggaaagaaa
     3241 gtcggagttc aggagaagtt gcatcgctta tgagaacatc acttggtaca tcttcaggtg
     3301 tatattcaaa acttgtatta tctctgactt ttcagaacag acattccgat ggaaccatgg
     3361 gaaacgtatt cagtctgaaa gactggtccg gtaattccaa tattttttat aacaggggaa
     3421 tctttaatca taatcttagc aacagaccat ctaccacaat ttttacaaat cttagtgcaa
     3481 atctggatgg taataatgat tacctggcag cagctgatga aagttccttt tcaccgactg
     3541 ctgtatttac acttgaagca tatgtttatc caaagtcatt cagtatcggt gaagtgatca
     3601 ttcataaagg attggataac ggattagtta caaattatgg attaagatta aatcagggta
     3661 atgtagtagc atatataaat aatcaggtca atcttatttc acagaatagt ataccattaa
     3721 ataaatggac tcatctggca tatgtttata atacaatttt cgggacaaat ttattatatg
     3781 taaatggagt tttaacagat tctgcaagca gcaataccgg tcttgtcacc aatggcaatg
     3841 attcatttta tgtaggaggt actattgctt tgcctgattt taacggatac atagatgaag
     3901 taagaattaa atctgcaata aaaaattaca gtgagatcaa tcagtttttg tacaaatcaa
     3961 tagatgagtc gaatgatctc aacggtactg aactggtttt taatcttgac ggaggtactc
     4021 tttcaaatgt tggtttagct attgatttaa aattcaggaa cgatgcagaa tttggaaatc
     4081 ccggtacttc atatgatata ccggtttcac cactacatcg tgcagataat atagatttca
     4141 atgaaggatt tattttgaaa aactcagaca ggagaattcc gggagcaggt acttcaggaa
     4201 atatgactga tgattcttta tatgtatatc aaaatgatat aataaatgac atcaatgtct
     4261 ttgtagcact taatcataaa aatgaaaatt ccctttcgat tacactggtc tcaccaaatg
     4321 gtacaagtat agatctatat aataataata ataatttttt aggatataat aacagtatag
     4381 ttacagtatt tgatgatgaa tccaattatt taaattcaaa taacacttat gtttcttttg
     4441 aaccatcaat aaagccgcag tcaaacataa attctgcatt ttcgggattg tcgagcttcg
     4501 gatactggaa gcttttagta aatgataatt ctcccggaga tactgggaga cttgtaagct
     4561 ggggaattca gatcaatgaa agtgtaaata aaaatttcaa tctgaattca acttcaataa
     4621 ttcagggatt ttataatcct ttgactaata cttcagtcag agatacaatg cagatcaagt
     4681 taagaaaatc tacttcacct tatattgtaa tagattccgc caaaagcttt tatttgccat
     4741 ctggattttc aacttatact tttaaaaatg ctgtagccgg taccccttat tatttacagt
     4801 tagaccatcg caattcgatc gatacatgga gcagtaattt tattacattt gaagcattgt
     4861 caggtcagag taattacaat tttacgttta actcagctca ggcatttgga aataatctga
     4921 tgcaggcaga tacttcacct cttagatttg ctatatataa tggtgatgta aataaagatg
     4981 gaattgtaga tgtgactgac aattcattgg tcgacaatga tgcatttaat tttaatacag
     5041 gttatatagt cacagatctt accggagaca atacagttga tctatcggat gcaacaatag
     5101 tagacaataa cgcctcaatg tttataagta agatcacacc ttaaaattca tttcatttcc
     5161 attccctcac gaaaagtttt ttcatttgtg agggaatgga atccttttta acactgtcca
     5221 aaatatttgt aatattattc agccgttgtt ggagttccta taaattttga taattttagt
     5281 gtgccgaata gatttgagat gctttgaccc tgattaatat tatgtatgat tgttgaaaaa
     5341 ttgatatatg gagatccgag aaaattaaaa tacccagaat gactagcaca tttcaatatc
     5401 cattgaatta cgactccggc aaagtctcaa atgatctcgg gagtctccgc cgggattaaa
     5461 atctgcttaa aattatattt agtattgtat ttgtaaatta ctgtaactac ttttacatac
     5521 atgacgtatg taaaaatcat tttaacatga aaagaacaaa gcaggaagct gagcagacaa
     5581 agaatgaatt acttgatgtt gctttcaaag agtttttgga aaccggttat aatgaatgca
     5641 gactggaaga tatcgcagca aaagcaggcg ttaccagagg agcattatac tggcatttta
     5701 aaaataagga tgatctgctc gattcactga tcagtcaaaa agatattgaa tcaaataaaa
     5761 ttgctgatga aattgccggg tcagatgatg agccttttga gaagttaaaa aagtttgtta
     5821 atctgaattt tccagatctg tcgggaggca ggaaagaaaa gaattatgtc aaactgaaag
     5881 ttgaacttta caaacatttc aatgaaaaag gggacaagaa taagagtgct gaaaaattta
     5941 cggcacattg taaaaagttt attgagcagt gtaaatcaag aggtgagata aaagatgaaa
     6001 tagatcctgc agatgctgct aatacaataa tgataatatg tgcaggttca tatataagat
     6061 ttaattctat acaatcaaat aagagggcaa ttaaatattc aaaaaaactt gcactcgatt
     6121 atctatattt aatacataaa taaaaatatg aaaaaattaa ttctgctatt gtttatcttc
     6181 gcaattgaaa tctttctgtc tgtcggcctt aaggcagcaa attttgacag tacttttgcc
     6241 gaacagcttc agcaacgtat caatctgtta aagacaaata acaatctcgt tggaatatct
     6301 gctgctgctt atgtacccgg gcaggggatg tggacaggtt catacggaat ctcatcttca
     6361 gacagtgatg agatcacaac ggaaatggtt ttgaattccg gaagtgttgc caagaatttt
     6421 atggcaacta ttattcttat gctggctgaa gctgacagcc tgaacctttc agatactctt
     6481 ggacaatggc tgtctcctta taataatatt ccgggtaatg taactatcaa acagcttctg
     6541 gatcattcaa gcggtattta taatgtaact gacaatcctg catttatttc tgctattaat
     6601 tcaaatctaa acagattatg gacgatcgag gaagtactca aaggcggtta tatactaagt
     6661 ccttattttc ttccgggtgc agcttttaga tattccaaca caaattatat gatccttgga
     6721 ttaataataa agaaaataat gaagactact ctggctgagc aattcaaaga aagatttttt
     6781 aaaccattca gtctgaatga aacttacttt gaatttgatg acactgttaa aagtccattt
     6841 gctcacaact gggctgactt taccggatca ggagtcagac aggatgcctt tcatattccc
     6901 acgacatcta taaacagttc aacaattgga gccggaggcg tggtcagcag acctgaaaat
     6961 ttggcaagat ggctgattgc actttttgaa ggtcacattt taaatgctaa ctcaatgacg
     7021 cagatgctta ctttcagaaa cgtcagcata gccggtggaa acggatatgg cttaggggtg
     7081 atccgataca atgttccggg aaatgtatgt tacggacatg gcggaaatat atttggatat
     7141 tccactgtgg caatctatga tcctgttgac agtatatcaa tcgctgttat gatcaataat
     7201 gatgtcaatg ccggacctat tggaattaat tttatgagtt ttgtaaagca gaataatcct
     7261 gtcagtattt ctgaaatatc agacgcaata ccggaaaaat atacacttga acaaaattat
     7321 ccaaatcctt ttaatcctgt tacaaatata aatttcagta ttccccatag aagttttgtg
     7381 aagattacag tatttgacat tcttggaaaa gaaattcaga atctgatcaa tgaagaactt
     7441 aatgaaggta actttaaaat cgattttaac ggaagcagtt taccttccgg aatttatttt
     7501 tataaaattg aaacaaatga ttttgtgcag gtcagaaaaa tgacattgtt gaaataattt
     7561 taacatccgg cagatttcca aatattcttg ggaaactctg ccgggagtga aatttgtaat
     7621 tcataaactc gcatagctaa aaccaaaagg aaatataaaa cagttattgc tcttcacctt
     7681 tttccaaagg ggaggctggg agggctttgt aaaattagtt tattttgact acacaatttt
     7741 tggtgttaaa tttttgcagt ttgttgtttg ttatttcctt ctgcaagaaa ccccttcact
     7801 cccccttttt taaaagggaa ttacttttga attattctga aacgtgctgt ttgaaatttt
     7861 gaattcattt ctaaaaaatt aattaaaaat tttcttacca ttttgcgatt aatttacttt
     7921 gtaacagaat aactcgtaat ttttaattgc actcctatgg tgtgatttta ctcacaaaat
     7981 taaacccgtt attatcagca aacacagcat ctcccacatc cactacactg tctccattta
     8041 aatcagtagg taaatatcct gaagcaaaaa ttaatccgtc attaaagata agactcatgt
     8101 ctgtaagatc aacaattccg tcctgatcta catcaccgct gtaaaatgca aatcttacag
     8161 gcatgttatc tacctgtaac atattgtccc catacgcctt cgttacatca agtgaaaatt
     8221 ggtaagaact atactgattc gtaaactgga atgtactact gctccaggtt tcaagagaat
     8281 ttctatgctt tacctgtaaa taataattga ctaagttatc agtattatag aattcaaaaa
     8341 atccattgcc tccgtttcct attgtggctt ttgatgaatc aagaatatta tatggagatg
     8401 aaatgcttct taaatataat ctcactgtat ctgatatgtt cataatattt gaagcagaat
     8461 tataaaaacc ttctatcaat ccgttgaatg aaagatattt gtatgtgcgt ggcgtaccgg
     8521 aacatcctaa agatgcccag acatttgtac ctccgctgta ttctgtccag acagcaaagc
     8581 agctttctcc gtcaggaccg tatttaattc ccggtcttgg ctgcgcagag aatggatttg
     8641 tgtctacatg attcatttga aaaactaatc ctacccatat attattttca gctgaacaat
     8701 aagtaaccgg tataatcgat tgtgtatttt cagaataagc gcatgaataa aatccgggag
     8761 cgcttctgaa tccgatgatg tctgctcttg ttgaattgtt gccccggtaa tctacatatc
     8821 cgtttatcca tcctgcgctt ccgtttgttg aacggtagta ttctatatcc cagtcagttg
     8881 ccgaatattt tctcatgttg actatcatca gattgttgta tcctcccgct gaagcaatat
     8941 atgcctgata tcctcttttg tcaggtattg tgctgatgtt tccgacgtaa gttgcaaagg
     9001 atgaagaaat aaatgaactt atggaagttt ttgctaaaac tattttcgat gagtcttcca
     9061 gacttgattc gactatcaat attgaatcct gtcctccgtt tttgaaataa gcaatatcaa
     9121 cataataatc cacaggatat ctgaatagca atcctaaaaa tccatttggt ttataagtaa
     9181 atgtcgggaa tacggtataa ggtgaataac aaattgctac tttctcacct gagtaatatc
     9241 cgcctgcaac ggcactgtca agacatgcgg taatatatac ccatggattc gtacgatatg
     9301 cttcgttgtc agaaactatc ctcggtttcc agtagtaatt tgaattaacc atacccggcc
     9361 atgcaagtgt atatccgctg taatttaata gaccggtaat ttttacaatg gtaactccta
     9421 tccggtattg cccgctgtaa ttatttgtgg catatccgaa tgctacccag ataaatttat
     9481 ctccggtact gttttcaatc agctcaatgt caatctgatc ctgccacatt ctgttgcctg
     9541 cagaaaatcc tacagttact ttttctatcc atgtcttacc gccgtcatcg gaaaagcaaa
     9601 gccgaagcgc atcggttgta gttccgcttt gtggtccgaa tacaaatgct gtccagattc
     9661 tgcttgcggt agatccaatt tgttccgtgc aggatgcaac tccttttgta cctgtaacag
     9721 gagagaccag cccgatactt atattgtcgg actgagtatt ttcagattta attaaatgca
     9781 aaggaaattc ttctcccggc tttgtgagag agccggttaa tgcatcaagc tcttttacaa
     9841 tttccattgt cccgtcaata tttgacgatt gttttgccag caaaagtttg ctttcgagat
     9901 cggtaatttc ttttgaattt ctgttgtctg tcagatcttg gtttctgtta aactctatcc
     9961 ttggttcagc agacttttct ttcggctgaa gtcctttgtt ttgtgaataa gcaaaactgc
    10021 ttattaatag tatgaaaaat aaattgatta actttgataa cattttacct ccagtggtat
    10081 ttataaagtt tttaaaataa actgattcat ccaaaacctg tgaaacagat ttcctttcat
    10141 tcagatgaac ttgtacaaaa ttgacataaa tacatttaca aagcaaagaa acttttcccg
    10201 gtttttcacc tcgaaaatca aattgttatt taggaatatt aatgatattt tctaaatgac
    10261 attccaacta tacaataata tgcttaacag tactctgctc gatattctca gaactttcga
    10321 caaagatcag ataaagaaat tcggaatatt tttgatctct ccttatcaca ataataattc
    10381 aaatgtaata aaacttttca attcattaaa aacatattct cctgaattta ttaatgccgg
    10441 actggaaaga gagatacttt ggaaagcatt attccccgga agagattttc attacggtgt
    10501 gatgaaaaat ttaatttacg aacttcagaa acttactgaa aaatttctat cattcgaaaa
    10561 atacagctct aacgaatttg aatttgagat gaatttactc gatgcattac ttgataaaaa
    10621 actgattact ctttttacca aaaatgcaaa aaatcttaaa gtgcaaatgt taaaatcaaa
    10681 tattgatctg aattattttt acaacaaata tattcttgaa tcaagagaac tcaactattt
    10741 ataccttaac aaaaatgcag ctaacaaacc cggcagttcc accggtaatc caaatgagag
    10801 tttgttaata tttttcttta ttaatttttt taacagtaat tataattcac tgcatgaaag
    10861 tatattatat aataatccct atgacacaga atacgtgaat tcagtaaccg gttattttaa
    10921 tataagtcct gtaaaaaaaa ataaatatgc gtctatgttt tcatctgtaa ttgacacact
    10981 tttaaatcca aacgatgaaa gtatatactt tggacttaag aatttattcg gaaagaattc
    11041 aggaagactc ggtaaggaat taagatataa tgttcttaca gcattgataa atttttgcaa
    11101 tttccagatc atgaagggaa atacaaagtt cataaaagaa caatttacta tttataagat
    11161 gatgattgaa agcggaaatt ataataacgg caaagataat tatatcaatc cttacatgta
    11221 tattaacatt gtcagcatgg cagccaatct tcgtcaatac ggctgggctg aaaaattcgt
    11281 tgaaagattc aaaaccaaac ttgaaacgcc tcatcttgat cagtgtttta actttgctat
    11341 ggtttctctt aatcttaaaa gaaaaaaatt tgatacagca atggaatatt tatccaggtt
    11401 gaaaagcgga gatgtgatag ataaaattac gatcagaaga tttcaactga tgctgtatta
    11461 tgaatcggga tatttcgatg aacttcattc attgatagat tcttcaagac atttcattac
    11521 caatgatcat aaaacttcag cacgggcaaa aagtattttc aaaaactttt cttcaataac
    11581 catttcaacg ttattcctgt tga
//
LOCUS       DAORVQ010000102        11421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2851_length_11421_cov_4.633732, whole genome shotgun sequence.
ACCESSION   DAORVQ010000102 DAORVQ010000000
VERSION     DAORVQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_11421_cov_4.633732"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3284
                     /locus_tag="PLX80_09240"
     CDS             <1..3284
                     /locus_tag="PLX80_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQY21011.1"
                     /translation="SDNDSEKKNEDENKKAERINSTIQDFIIAISIFSFIVIGLSLFI
                     SERLIKNKSKAEDLLMKSYEEMESRVEDRTEKLREANEKLSNEVSVRKKIEDNLRESE
                     YRFRIMADSAPVLIWMTDNNKQFSYFNREWLNFTGRTMEEESGYGWLHGMHPDDLQKC
                     IQIYEDSFDKRIPFDMEFRLKNNTGEYIWIYSKGTPRYEGNYFAGYTGSCTDINERIK
                     NEKFLKIQYEISKTLSESKTLEEASNKLLENICTGMDWDFGILWTIDDNHEFVKINSL
                     WGKDENSIREFENSNDTKKTFSKDKGFQELLDKGKSVWKKDMHFIEDPNFKYDPKKMG
                     WLSGLEIPISSGTEIIAMIECLSKKSMEERLDFIAVLESAARQIGNFIERKKAENRLL
                     KSNLELEENVKIRTAELVNALSKLIKESEEKEKIQNKIKLFAHTIKSMKDSVFIADQN
                     NNILFVNEAFEQTYGFEDKELIGKEVPILKDIIPEIKNKILKSSFENGWKGELLTKKY
                     DGTNFYTYLSTSAIKNDDGSANAWVGICQDMTEIKKAEDLILKRNNLLLVLNDVISYT
                     NNIFDLNSGIQYSINKICEYTHWEIGHCFLIKNDKFVSSKIWNKDMPESSYKFKEISE
                     NLIFNKGEGYPGKKLLDQKSEWIKFREIKEKNIILRLDVVNELGLKTGIWVPIFMNNE
                     IIGILEFFKIDEEEIDPEILNCINNIGIEIGSLYDKIETINKIRQSENKLNDAQHIAK
                     LGNWEWDVINNKITWSDELYRIFGVKRDDFKPTFENYLSGIHESDVDRVQKIIQNALI
                     NKTTFNFYHRLKSQDGKEKIINSQGEVHLDEDNNVIRMFGTAHDVSEIRQAEEELRIT
                     NAKLIATQKELIYNEKLAALGRFSSGVAHEIRNPLANISSLAQMITKADIDVKNKRRL
                     NYIITNVDIANKIIKSLLNFASPGELKYSSVNVIDILENIIESVEARCKSSDINIIFD
                     SPDKIPTMNIDKLKLEAALMNFVSNAIEAMNEGGDLTIKVRLESESEMIRIEFIDTGI
                     GIPAENLDKILEPFFTTKDEGVGLGMGLAYQTIKSHHGTYEIDSVLGKGTTIKIKFPL
                     TNNKL"
     gene            3287..4636
                     /locus_tag="PLX80_09245"
     CDS             3287..4636
                     /locus_tag="PLX80_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY21012.1"
                     /translation="MEKVLIVDDNETLRFTLTELLEESDFICKAVENGNLALKELTKD
                     QSYGLVILDMRLPGMNGLEILREIKKINKLLPVIMLTAYGEIKTAVEAMKAGAHDFIT
                     KPFDNEAIIITIRKTLELKYLNQEVNLLRKKIDSSNIKGEVIGECDAMKKVFEQVKIV
                     SPTKLSVLLEGESGTGKEVIACMIHNLSERKDKSFIAVDCGAIPESLIESELFGHEKG
                     AFTDAKSAKEGKFELANEGTIFLDEITNLSDSNQIKLLRVIQDRKVTRLGAKSPVKLN
                     IRIISATNIKLADAVNNKKFRQDLYYRLNEFYLELPALRDRKDDIKLLVEHFIKESNQ
                     ELNKNIKSVSDKVMEKLVSHSWFGNVRELRNVIRRGVLLNKGDEIKSIDIPDEIKFNN
                     SDVSESKISYSDAKQDAEKELILDALKKAGDNKTIAAKILNMNERTLYRKLKKLGIT"
     gene            4937..5362
                     /locus_tag="PLX80_09250"
     CDS             4937..5362
                     /locus_tag="PLX80_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21013.1"
                     /translation="MNNKRVSNGETKSEIFETKNETDVELGKDLSLDIFPNEEVIPDG
                     PEISLSDYTISLLDHEDNKYKTVKEIILEEELRKKDDKIYKLRHQKLETDNNASDKES
                     VLNPDIEKITDDDPDERKVSENRENKILNNSRNRNQMFL"
     gene            5657..5968
                     /locus_tag="PLX80_09255"
     CDS             5657..5968
                     /locus_tag="PLX80_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HQY21014.1"
                     /translation="MEKKIRTLVESIEEFRKKEYTEDFIVENDLLKCKETGESFWPED
                     VKIESTERYEGDSNPDDMSIIYAISSSSGTKGILIDAYGVYADLSISEFIKNVPVERV
                     K"
     gene            5986..6756
                     /locus_tag="PLX80_09260"
     CDS             5986..6756
                     /locus_tag="PLX80_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY21015.1"
                     /translation="MKRVYMEKLFPLSSRYNESWVRRNSLGENVLYNLECLCEILNMN
                     SGMKILDLGCGKAISAIFLANEFNSKVWAVDPKMNPSSNLNRIREMGCEENILPLKLE
                     ANALPFPEEFFDLIIAVDSFMYFGTDFGFTDYITKFLKPGGQIGIVDICTHNEQYEKD
                     RIAEKKINIDDNPDYIKSLDWWSNLWNDCPDLDLKFCEIVPENDVIIKEYIKDFKYSN
                     KTDMLADELDHDENNLLKIFRMSAVKKLKSDNFYNVIN"
     gene            7049..8806
                     /locus_tag="PLX80_09265"
     CDS             7049..8806
                     /locus_tag="PLX80_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HQY21016.1"
                     /translation="MHLLDWVIVCVYFIIALAIGFYYSKRAGGSTEEFFLSGRKLTWW
                     LAGLSMVATTFAADTPLAVTELVGQNGIAGNWLWWNMLIGGMLTVFFFARLWRRAEIL
                     TDVEFIELRYSGKPAAILRGFKAIYMGIFMNTIIMGWVNLAMIKILIGMFPEYVNPDN
                     AIYYVGACMLFTAFYSAISGLWGVAVNDAFQFILAMGGCIVLAVIVVNSPEIGGISGM
                     QEKLPEWALKFTPEISSGDGETSTGGILALTISSFLAFVGLQWWASWYPGAEPGGGGY
                     VAQRMMSAKDEKNSLFATLLFQVAHYCIRPWPWILIGLCSLILYPDLSQADKGLGFVM
                     AMNDFLPVGLKGLLLAAFFAAYMSTIATHLNWGTSYFINDFYRRFLSKDKSEKHYVVV
                     ARLMTIVFMFLSVIVTTQMTSISGVWSFLIECGAGLGLVLILRWFWWRVNAIGEIVAT
                     VTPFVVYGVLYFGDFGVKFPETLFIIVPITTLAWIVTVYITKPTATAKLDSFYRRVHP
                     GGIGWKIISDKMTDVKSDSGYYLLMINWVAGIIMAYAFLFGIGKVILGDTTTGILFLI
                     TGILAAFLIKRNFAKTGWN"
     gene            9076..9528
                     /gene="bcp"
                     /locus_tag="PLX80_09270"
     CDS             9076..9528
                     /gene="bcp"
                     /locus_tag="PLX80_09270"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HQY21017.1"
                     /translation="MLKIGDKAPAFTLISDTGEKVSLKDLKGKKVILYFYPKDDTSGC
                     TKEACSFRDNIKVIEKKNAVVIGVSKDNTKSHQKFKLKYDLPFTLLSDENFEMLKEYD
                     VWKEKSMYGKKYMGIERTTYVIDEKGKITDIFNKVNVDGHTEEILAKL"
     gene            9567..10700
                     /locus_tag="PLX80_09275"
     CDS             9567..10700
                     /locus_tag="PLX80_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21018.1"
                     /translation="MSDKIEIIEVLTEAEKKKFIEFPYHLYKDNKYWVEPLRFDVKNN
                     LDEKKNPFYTHAKIKLWLAYKNGEIAGRIAGITNNAHNKFYNDKVGFFGFFESVNDKK
                     VSEPLFQKAAEFVKENGMDTLRGPVNPSTNDECGLLTDSFDQPPVMLMPYNPEYYIGL
                     YGDFGLKKAKDLYAMWISKDVIKNEAMMNKLDRISKMIIKKENLTIRNVNMKDFKNEV
                     QKVREIYNNAWQDNWGFVPMSEAEFNFIAANLKLAVDPDYVEFAEIDGKPIGFSLALP
                     DVNQAIKGLNGKLFPFGFIKFLKNKKKIDQLRVIIMGVKKEYHKKGIDAVFYRNIIQA
                     ADRKKIRGAEISWVLEDNFAMMQTAVKLGAEVYKTYRIYDKAL"
     gene            complement(10695..>11421)
                     /locus_tag="PLX80_09280"
     CDS             complement(10695..>11421)
                     /locus_tag="PLX80_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HQY21019.1"
                     /translation="YQDDINTSKEVLYQIHAAHMLHIPFENLDIHLKNKIILDKDHLF
                     KKIIENKRGGYCYELNGMLYLLLKEIGFDVKMISAQVSIGEDLWTPDFSHLALLVKLD
                     NTYLTDVGFGDNFFEPLLIENDIIQNDKAGYFKIVKDGEDYFKLMNSPDGNDFKDGYR
                     FTKNEYKWNDFEEMNIYQQTSEDSHFIKNRICSLATKSGRISLSNNKLTITENGEKKT
                     REFNNEDENEFNKLLFEKFQIKL"
BASE COUNT         4303 a         1536 c         2131 g         3451 t
ORIGIN      
        1 tttcagataa tgattcagaa aaaaagaatg aagatgaaaa taaaaaagca gaaagaatta
       61 attctacaat ccaggacttc attatagcaa ttagtatttt ttcatttatt gtaataggct
      121 tatctttatt catttcagaa agactgatca aaaataaaag taaagccgaa gatctgctga
      181 tgaaatctta tgaagagatg gagagcagag tagaagacag gactgaaaaa ttaagagaag
      241 ctaatgagaa gttatcaaat gaagtttccg taagaaaaaa aatcgaagat aatttaagag
      301 aaagtgagta cagattcaga ataatggctg attcagcacc tgtattaata tggatgacgg
      361 acaataacaa acaattttca tattttaaca gggaatggct aaattttaca ggaaggacaa
      421 tggaagaaga atccggatat ggatggcttc acggaatgca tccggatgat ctgcaaaaat
      481 gcattcagat atatgaagat tcttttgata aaagaattcc atttgatatg gaattcagat
      541 taaaaaataa cacaggggaa tatatctgga tttatagtaa aggaacacca agatatgagg
      601 gaaattattt tgcaggttat acaggatcct gtactgacat aaatgagcgg attaaaaatg
      661 aaaagttttt gaagatacag tatgaaattt caaaaactct ttccgaatca aaaacacttg
      721 aagaagcttc aaataaattg ttggaaaata tatgtaccgg tatggattgg gactttggaa
      781 ttttgtggac aatagatgat aatcatgaat tcgttaagat taattctctt tggggaaaag
      841 atgaaaacag tatcagggaa tttgaaaatt caaatgatac aaaaaaaacc ttttccaaag
      901 acaaaggctt tcaagaatta cttgataaag gaaaatccgt ttggaaaaaa gatatgcatt
      961 ttattgaaga tccaaacttt aaatatgatc caaagaaaat gggctggcta tccggactcg
     1021 agatcccgat atcgagtgga actgaaataa tagcaatgat agaatgtcta agtaaaaaaa
     1081 gtatggaaga gagactggat tttattgctg tactggaatc cgctgcaagg cagattggga
     1141 attttataga aagaaaaaaa gcggaaaata gacttctgaa atcaaatcta gaattagagg
     1201 aaaatgtaaa aataagaact gctgaactag taaatgcttt atcaaaactt ataaaagaaa
     1261 gcgaagaaaa ggaaaaaatc caaaacaaga taaaactttt tgcccatact attaaaagta
     1321 tgaaagacag tgtgtttata gcagatcaaa acaataatat actttttgta aatgaggcat
     1381 ttgagcaaac ttatggattt gaagataaag aattaattgg aaaagaagta ccgatcttaa
     1441 aagatataat tcctgaaatt aaaaataaaa ttctaaagag ttcttttgaa aacggctgga
     1501 aaggtgaatt acttacaaaa aaatatgacg gtacaaattt ttatacatat ctttctacat
     1561 cagcaattaa aaatgatgac ggatctgcaa atgcctgggt aggtatttgt caggatatga
     1621 ctgaaattaa aaaagcagag gatttaatcc taaaaaggaa caaccttttg cttgttctca
     1681 atgatgtaat tagctacaca aataatattt ttgatttaaa ttctggtata caatattcca
     1741 taaataagat atgtgaatat acccactggg aaattggaca ttgttttctt attaaaaatg
     1801 ataaatttgt ttcttcgaag atttggaata aagatatgcc ggaaagcagt tataaattca
     1861 aagaaatttc tgaaaactta atttttaata aaggtgaagg ttatccgggt aagaaattat
     1921 tagatcaaaa atcagaatgg ataaaattta gagaaattaa agaaaaaaat attatcctga
     1981 gactggatgt tgtaaacgag ctgggtctca agaccggcat atgggtgccg atttttatga
     2041 ataatgaaat aataggtatt ctggaatttt ttaaaataga tgaagaagaa atagatccgg
     2101 aaatactaaa ttgtattaat aatattggaa ttgaaatcgg aagtctttat gataaaattg
     2161 aaactattaa taagatcaga caaagtgaga ataaactaaa tgatgcgcag cacatagcca
     2221 aacttggtaa ttgggaatgg gacgtgatca acaacaaaat tacctggtcc gatgaattgt
     2281 accgaatttt tggtgtgaaa agagatgatt ttaaacctac ttttgaaaat tatctcagtg
     2341 gaatacatga atctgatgta gatcgagttc aaaaaatcat tcagaatgct cttattaaca
     2401 aaacaacttt caacttttat cacagactta aaagtcagga cggtaaagaa aagatcatta
     2461 attctcaggg tgaagtacat cttgatgaag ataataatgt catcagaatg tttggaactg
     2521 ctcatgatgt atcagagatc agacaagcag aagaggaatt aaggataaca aatgctaaac
     2581 taattgctac acaaaaagaa cttatttata atgaaaagct tgctgctctt ggaagatttt
     2641 catccggagt agctcacgaa ataagaaatc cattggcaaa tatcagcagt cttgcacaaa
     2701 tgatcaccaa ggcagatatt gatgtaaaaa acaaaagaag actgaattac attataacaa
     2761 atgttgatat tgcaaataag atcattaaaa gtttattaaa ttttgcatca cccggagagc
     2821 tcaaatacag ttctgtgaat gttattgata tccttgaaaa tataattgaa agcgtagaag
     2881 caagatgtaa atcgtctgat atcaatatta tctttgattc tccggacaag attcctacaa
     2941 tgaatattga taaacttaaa ctggaagcag cattaatgaa ttttgtttca aacgctatag
     3001 aagcaatgaa tgaaggaggt gatctgacga taaaagtaag actggaaagt gaaagtgaaa
     3061 tgataagaat agaatttata gatacgggta tcggaattcc cgcagagaat ttagacaaga
     3121 tccttgaacc attctttaca acaaaagatg aaggtgtcgg actgggaatg ggacttgcat
     3181 atcagacaat caaatcacat catggaacat atgaaataga tagtgtttta gggaaaggca
     3241 caacaataaa aataaaattc ccattaacta acaataaatt ataataatgg agaaggtatt
     3301 aattgtagac gataatgaaa cattaagatt tacacttaca gaattacttg aagaatcaga
     3361 tttcatctgc aaagcggttg aaaacggaaa tcttgcatta aaagaattga caaaagatca
     3421 gtcatatggt cttgtaatac ttgacatgag acttcccgga atgaacggat tagaaatttt
     3481 aagagagatc aaaaaaataa acaagttatt gcctgtgatc atgcttacag cttatggaga
     3541 aataaaaact gcagtcgagg caatgaaagc aggtgctcac gattttataa caaagccttt
     3601 tgataatgaa gcaataatta ttacaattag aaaaactctt gaactgaagt atttgaatca
     3661 ggaagtaaat ttgcttagaa agaaaattga ttcctctaat attaagggtg aagttatagg
     3721 tgaatgtgat gctatgaaaa aagttttcga acaggtaaaa atagtttcac caaccaaatt
     3781 aagtgtactg ctcgaaggtg aaagcggaac cggtaaagaa gtaatagcat gtatgatcca
     3841 caaccttagt gaaagaaaag ataaaagttt tatagcagtt gactgcggag caattcctga
     3901 aagtcttatt gaaagtgaat tatttggaca tgaaaaagga gcatttacag atgctaaatc
     3961 ggcaaaagaa ggtaaatttg aacttgcaaa tgaagggact atctttttgg atgagatcac
     4021 caatctgtct gattccaatc agataaaatt actgagagtc atacaggaca gaaaagtaac
     4081 cagactcgga gctaagagtc cggtaaaact aaacatcaga attatttctg ccacaaatat
     4141 aaagcttgca gatgctgtaa acaacaaaaa gttcagacag gatctgtatt acagattgaa
     4201 tgaattttat ctggaattgc cggcattaag agacagaaaa gatgatataa aattactggt
     4261 agaacatttt ataaaagaat caaatcagga attaaacaaa aacattaaaa gtgtatctga
     4321 taaagtcatg gaaaaactcg tttcccacag ttggtttggg aatgtcagag agctaagaaa
     4381 tgtaattaga agaggagttt tactaaacaa aggagatgag attaagtcta tagatatacc
     4441 cgatgaaatt aaatttaaca attccgatgt atcagaatca aaaatatctt attcagatgc
     4501 aaaacaagat gctgaaaaag aattgatatt agatgctttg aaaaaagccg gtgacaataa
     4561 aaccattgca gcaaaaatat taaatatgaa tgaaaggaca ctttacagaa aattaaaaaa
     4621 acttggaata acataatttc aataattcta ttccatactt ttcatttttc aataagtact
     4681 gttcaatatc tcaattttta cagattgatt tgtccgtctg taatccccca aattctaaaa
     4741 tacaaattat atattttctc ttcattttca ttcttaatta cgacagtttc agacactatt
     4801 gtcataaaat atattaccgt aatatatatt aatgtgataa aacttataaa aattaagttt
     4861 caatttatgg cacactatat gcatactcat ttgtaaagtt taataaacaa tttcataaaa
     4921 taaattggag tttattatga ataacaaaag agtcagcaat ggcgaaacta agagtgaaat
     4981 ttttgaaacc aaaaatgaaa ctgatgtaga attaggaaag gatctttctt tggacatttt
     5041 cccaaatgaa gaagtgatac cggacggacc ggaaatttca ctttcagatt atactatcag
     5101 tttattagat cacgaagata acaaatataa aacagtcaag gaaataatac tggaagagga
     5161 gctgagaaaa aaagatgata aaatctataa acttagacat caaaaactag aaacagataa
     5221 taatgcttca gacaaagaat ctgttttaaa tcctgatata gaaaaaatca ctgatgatga
     5281 tccggatgaa aggaaagtca gtgagaacag agaaaataaa atattaaaca attctagaaa
     5341 cagaaatcaa atgttcttat aattccatca atagaaatac ttccatcaat aatcctgccc
     5401 tgtttttatg atcattgggg gatgatcggg gcaggttcct ttttatactg aaatacaaat
     5461 gttaatgtgg ggaaataaaa caaagtaatc aagtatactt tcaccgcaaa aagggaacga
     5521 gttattctaa ctaattcatt gattgaagaa ttgcaaggga gtctataaac aaatgccaaa
     5581 agcacttaag agtcaaattt gtactgaact ttttttgaaa ttaaaattaa aaatcaatta
     5641 atcaaggagt ttaattttgg aaaaaaaaat aagaacactg gtagagtcaa ttgaagagtt
     5701 cagaaaaaaa gaatataccg aagattttat agttgaaaat gatctgctga aatgtaagga
     5761 aacaggtgaa agtttttggc ctgaagatgt aaaaatagaa tcaactgaaa gatatgaagg
     5821 agattcaaat ccagatgata tgtcaattat atatgctatt tcatccagtt caggtaccaa
     5881 aggtatttta atagacgcat atggagtgta tgcagattta agtataagtg aattcattaa
     5941 aaatgtacct gttgaaagag taaaataaaa tttaaaaaat ctatcatgaa aagggtatat
     6001 atggaaaagc tttttccttt aagctcaagg tataatgaaa gctgggtcag aagaaattca
     6061 ctcggtgaga acgtattata caatcttgaa tgcctttgtg aaattcttaa tatgaattca
     6121 ggaatgaaaa ttcttgatct tggttgtggt aaagctatca gtgctatttt tctggcaaat
     6181 gaatttaatt caaaagtctg ggctgtggat ccaaaaatga atccgtcttc aaacctcaat
     6241 agaataagag aaatgggatg cgaggaaaat attctgccat tgaaattaga agcaaatgca
     6301 cttccgttcc ctgaggaatt ttttgatctg ataattgcag tggactcatt tatgtatttc
     6361 ggaactgact ttggatttac tgattatatt acaaaatttt taaaacccgg aggtcagatt
     6421 gggattgttg atatttgcac acataatgaa caatatgaaa aagacagaat agctgaaaag
     6481 aaaatcaaca ttgacgataa tccggattat atcaaatcac ttgattggtg gagtaatctt
     6541 tggaatgatt gtcctgatct tgatttgaaa ttttgtgaga tcgtacctga aaatgatgtt
     6601 atcataaaag aatatattaa agattttaaa tattcaaata aaactgatat gcttgctgat
     6661 gaactcgatc atgatgaaaa taatttattg aaaattttta gaatgtctgc ggtaaaaaaa
     6721 ttaaaatcag ataattttta taatgttata aattaaagaa ggcaaataat aattacttcg
     6781 ttacttactt tcaatttaca tttcaatttt tcggaccgga ttataaatat acggtccgaa
     6841 atttctattt atctctctta tctctcttgt atttctgata tttcataaaa caaaatatag
     6901 tttttacaca ggcataatta tatcagcaga tactatgtaa atttttcatt gtcagaatat
     6961 tataattttt tcagattaga aaaattctat atttcaattt aagtttagaa aatttaaatt
     7021 ggcaaattaa tttacttatc ataagctgat gcatttgtta gactgggtaa ttgtctgtgt
     7081 atattttatt attgcgttag ctataggttt ctattactcc aaaagagcag gtggatctac
     7141 tgaagaattt tttctctccg gtagaaaatt gacatggtgg cttgccggac tatcaatggt
     7201 cgcaacgaca tttgcagcag atacaccgct tgcagttaca gaacttgtcg gacaaaatgg
     7261 aatagcaggc aactggctct ggtggaatat gctgatcggt ggaatgctta ccgttttctt
     7321 ttttgcaaga ttgtggagac gggcagagat attgactgac gttgaattta ttgagttaag
     7381 atattcagga aaacctgctg caattctcag aggattcaaa gcaatttaca tggggatctt
     7441 catgaacacg atcattatgg gttgggtcaa tcttgcaatg ataaaaattc tgataggaat
     7501 gttccccgaa tatgtcaatc cggataatgc aatatattat gtcggtgcat gtatgctgtt
     7561 tacagctttt tattcagcta tctccggact ttggggtgtc gcagtaaatg atgcatttca
     7621 gtttattctt gcaatgggcg gatgtattgt actggcagtg attgtagtga actctccgga
     7681 gataggcggt atcagcggca tgcaggaaaa acttcctgaa tgggctttga aatttactcc
     7741 ggagatatca tcaggtgatg gagaaacttc aacaggcgga attttagctc ttacaatttc
     7801 ttccttccta gcatttgttg gactgcagtg gtgggcatcc tggtatccgg gtgcagagcc
     7861 gggaggcggc ggctatgtag ctcagagaat gatgagtgct aaggatgaaa aaaattctct
     7921 atttgctact ttattatttc aggttgcaca ttattgtata agaccctggc cgtggatcct
     7981 gattggactt tgctcgctca ttctttatcc cgacttatca caggctgata aaggacttgg
     8041 atttgtgatg gctatgaatg acttccttcc ggtcggatta aaagggctac ttctcgcagc
     8101 attctttgct gcatatatgt cgacgattgc aactcatcta aactggggta catcgtattt
     8161 cataaatgat ttttacagaa gattcctttc aaaggataaa agcgaaaaac attatgttgt
     8221 ggttgcaaga cttatgacaa ttgtatttat gtttctgtct gtaatagtga ctactcaaat
     8281 gacaagtatt tcaggagtct ggagtttcct cattgagtgc ggtgcaggtc ttggactggt
     8341 tttaatatta agatggtttt ggtggagagt aaatgcaata ggggagatag tagcgactgt
     8401 cacaccattt gtagtatatg gagtacttta ttttggagac ttcggagtga agtttccgga
     8461 gacattattt attatagtac caattacgac tcttgcatgg atcgttacag tttacattac
     8521 aaagccgaca gcaactgcta agcttgattc attttacaga agagtacatc cgggcggaat
     8581 cggatggaaa ataatttccg ataaaatgac ggatgtgaaa tctgattcag gatattacct
     8641 gcttatgatc aactgggtag cgggaataat aatggcttac gcatttttgt ttggaattgg
     8701 aaaagtcata ctcggtgata cgacgacagg aattttattt ctgatcacag gcatacttgc
     8761 tgcttttttg atcaagagaa attttgcaaa aacaggatgg aattaattcc aaattaaaat
     8821 gcttccaagg ttcttaaagt ggatttaaac tttttaacag taccgttata ttaataaaaa
     8881 aactagaaat ttataatcaa taaaaatact tactatacta acattattat aaataacaag
     8941 cggggaggaa gaccctgaaa aaaaatatta ggtattagtg tatatgatgt attaccataa
     9001 atggcttcag aaatggaact ctctttaatc tgcaaacaaa agtttacaat cttataaaat
     9061 atacaaagtc taattatgtt aaaaatagga gacaaagctc cggcgtttac actaatcagc
     9121 gatacaggag aaaaggtaag cctgaaagat ctgaaaggta agaaagttat tctttatttt
     9181 tatccgaaag atgatacatc cggatgtaca aaagaagcat gcagcttcag agataatatt
     9241 aaagttattg aaaagaaaaa tgcagttgtg attggtgtaa gtaaagacaa cacaaaatca
     9301 catcaaaaat ttaaactcaa atatgatctg ccgtttactc ttctctctga tgaaaatttt
     9361 gaaatgctga aagaatatga tgtctggaaa gagaaaagta tgtatggtaa aaagtatatg
     9421 ggaatagaga ggactactta tgtaatagat gaaaaaggaa aaatcacaga catttttaac
     9481 aaagtgaatg tagatggaca tactgaagag atcttggcta aattataaat atttacaaat
     9541 aattttattt aaataaaaaa tcaattttgt cagataaaat agagatcatt gaagtattaa
     9601 cagaagctga aaaaaagaaa tttatagagt ttccgtatca tttatataag gataataaat
     9661 attgggtaga gccgctgaga tttgatgtaa aaaataatct tgatgaaaag aagaatccat
     9721 tttatactca tgctaagatc aaactctggc tcgcttataa aaacggagag atagcgggac
     9781 gtatagccgg aattacaaac aacgcacata ataaatttta taatgataaa gtaggattct
     9841 tcggattttt tgaatctgtg aatgataaga aagtttccga gccgttattt cagaaagctg
     9901 cagagtttgt aaaagaaaac gggatggata ctcttcgcgg tccggtaaac ccttctacga
     9961 atgatgaatg cggacttctg acagacagct ttgatcagcc gcctgtaatg ctgatgccat
    10021 ataatcctga atattatatt ggactttatg gagactttgg tttgaaaaaa gccaaggatc
    10081 tttatgcaat gtggatcagt aaggatgtta ttaaaaatga agcaatgatg aataaactcg
    10141 acaggattag taagatgatc attaagaaag aaaatcttac gatcagaaat gtgaatatga
    10201 aagactttaa aaatgaagtt cagaaagtaa gagagatata taacaatgca tggcaagata
    10261 actggggatt tgttcctatg tccgaagctg aatttaattt cattgctgca aatctgaagc
    10321 ttgctgtgga tccggattat gtagagtttg ctgaaataga cggaaagccg atcggttttt
    10381 ctcttgcttt accggatgta aaccaggcta taaaaggact taatggaaaa ttgtttcctt
    10441 tcggatttat aaaatttctc aaaaataaaa agaagatcga tcagctcaga gtaataataa
    10501 tgggtgtgaa aaaagagtat cataaaaaag gaatagatgc agtattttac agaaatatca
    10561 ttcaggctgc tgacagaaaa aaaattagag gagcagagat atcatgggtg ctcgaagata
    10621 attttgcgat gatgcaaact gcggtaaaac tcggagctga agtttataaa acttacagga
    10681 tctatgataa agctttatag ttttatctga aatttttcga aaagtaattt attaaattca
    10741 ttttcatctt cattattaaa ttcacgtgtc ttcttttcac cattttcagt aatcgtaagt
    10801 ttattattac ttaaagaaat tcttcctgac ttagtagcca gtgaacagat ccggtttttt
    10861 ataaaatgag aatcttctga tgtctgctga tatatattca tttcttcaaa gtcattccat
    10921 ttatattcat ttttagtaaa tctatatccg tccttaaaat catttccgtc aggagaattc
    10981 ataagcttaa aataatcttc accgtctttt acaattttaa aataaccggc tttatcattt
    11041 tgaataatat cattttcaat gagaagaggt tcaaaaaaat tatctccgaa gccaacatct
    11101 gttaaatatg tattatcgag ttttaccaat aaggcaaggt gcgaaaaatc aggtgtccag
    11161 agatcttcac cgatacttac ctgagcagag atcatcttta catcaaatcc gatttctttt
    11221 aatagcagat agagcattcc attaagttca taacaataac cgcctctttt attttctatg
    11281 atctttttga aaagatgatc tttgtcgaga attattttat tctttaagtg tatgtcaaga
    11341 ttctcaaatg gaatgtgcag catgtgagcg gcatgtatct gatacaaaac ttctttcgat
    11401 gtatttatat catcctgata t
//
LOCUS       DAORVQ010000103        11404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2861_length_11404_cov_9.922460, whole genome shotgun sequence.
ACCESSION   DAORVQ010000103 DAORVQ010000000
VERSION     DAORVQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2861_length_11404_cov_9.922460"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..985)
                     /gene="murI"
                     /locus_tag="PLX80_09285"
     CDS             complement(170..985)
                     /gene="murI"
                     /locus_tag="PLX80_09285"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQY21020.1"
                     /translation="MNNKISKRSPIGVFDSGIGGLTVAGSLFEILPNENIIYLGDTAR
                     LPYGSKSKETVIKYSMEITKFLLNKNVKMIVVACNTATSFALPSLKKLTDIPVLGVIK
                     PGCKAALSETKNFRIGVIGTLGTIMSDSYKTQIHRFESNIKVFSKACSLFVQLTEDGW
                     NDNKIAKLVAKEYLDEFKKLNIDTLILGCTHYPILKKVISEVLGKKIILIDSGEETAK
                     EVKIILGQKHLLNDQKKKGVHKFYVTDFQNKFKDISERFLGQPIGDVKKVKIN"
     gene            1093..3189
                     /locus_tag="PLX80_09290"
     CDS             1093..3189
                     /locus_tag="PLX80_09290"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21021.1"
                     /translation="MRSFFTNLFFCFSLFFIPSLTQSQWQWQNPIPQGNILFGSHFIN
                     DNTGYACGDGGTIIKTTDGGNTWAINKTKTKESIRSLSFTDENSGWACGLAGTILKTT
                     NGGVFWDRIISGTTGNLFCVRFLNSNTGIIVGDTGIVMKTTNGGNSFERKPTGISLSL
                     TSCSLMGNIFLAAGLNGAYARSADYGNTWQQISTGNNNYFFDIMFKDSVTSFISGAYG
                     TILRSTDAGLSWTRMSTLTGNWFYTISFGDAMTGFASSDYGNVYKTTNAGLNWTGLAS
                     TTSEAQYSLKAFDEDEIISFGNKGSIITTSNSGLNWSNITSGFRNTIYSLDFINDNTG
                     YGCGGQGSFIKTTNGGTDWIRSQAGSTNDFLFNIDMTNEFTGYCAGYLGKFLKTTDGG
                     ANWSAVNLNTERSINAVYFFNENTGWICGDSGMLLRSDDGFASSVSVPADQNFDITDI
                     EFVNSNVGYYSSQQRKIFKTTDAGENWFTPHTGDFGSTVGGLDFIDANTGIAFTYNNK
                     ILITNDGGVNWIEKLIPPGNYLNNVYISGHNNYWAVGDFGFIYRSTDGGAFWYEENDN
                     NFMDRIFGIYFKNNSEGWVSGGNGLIAEYYNPQSSIISLNQNNNTTGYKLNQNYPNPF
                     NPATVIQYELPVSGNISLKVYDVLGNEVAYLVNERQNAGSHSVKFDGNNLSSGVYFYK
                     LETDGFSDTRRMVLIK"
     gene            3289..6825
                     /locus_tag="PLX80_09295"
     CDS             3289..6825
                     /locus_tag="PLX80_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21022.1"
                     /translation="MKKLFLILPVALICFILMTGQNSTEDNPRWSIDPRSSVTYSNGD
                     YTSLPTTDIGIKQFTFAPRTIITPIGVMVASPNVRVHPSAGTQQNEVDIVRHPTNPNI
                     MFAAAQPINNANNFINVGVYVTTDGGLSWNGRDTMNAPNLNDQRGDPAPIIDKNGTFL
                     YGHLTSSANFGGLTGMGANFSTDNGATWSSTFQVMANANVDKNMTGTDGSPSSPFYGN
                     SYMAWTVFAGTAGNGRVSRTTNGGVSWDPVITLNSTPANHFAQGHDVAVAPNGNVFVC
                     WTAGSSTSPFTEDFVGIAKSTDGGLTYTATENAYDVNGSRSASFNGWGIRTNGFPRID
                     IDKSGGARNGWIYIVDGQINLAPAGSDADVIMRRSSDNGATWSAGIRVNQDALNNGKV
                     QFFPVINVDDAGGLNVVYYDNRDFPSVNDSCSVYMSRSIDGGNTWADVEVADHHFKPK
                     NLPGINTMGDYIGVTSGNGKVWPVWMDDKAGPGVQFNIWTTNIQISTFPLSSFNLTSP
                     AAGTTIATLPNSTTQYNFGWDTSASTASYKWIFGSPSASSRQITLPPSGNSLTVTGGQ
                     LDVILQGLGLNQGDSLVGSWDVWAFRNNQTNDSMKATNGPRTITLKRARPSLTAFNLI
                     SPPNNTSLLTLVTNTTPVNINWTKSGQAVNYRWLYASPNFSNQANVKLRIAANNSGFD
                     SLLTLRNSQVDSALAGLGVNVGDSTVGQWRVYGYSGSDSLASAQTYNITMRRGIPPTI
                     TSSADSIVVSLPVGGSASRNLIIGNTGQFPLDWVITESSSSLDNLNVITTKIDEALIL
                     QQANLPKGADDIYHGPDVTDGMGGPDAFGYRWIDSDEPGGPVFNWYEISAIGTQITTW
                     TSGTGDDGSVIVPLPFGFSFYGTSYNQLKICTNGWVGFDVASISNAYLNVAIPATSEP
                     NNAIYPFWDDHDVRTSGQIFYHNDAANNRFVIEYKDVPHFTSGGPYTYQVMLYSDGRI
                     IFQYLNMTDPLNSATIGLENSTGTVALQVAFNAAYMHNNLAIKLEKGLAWVDETPSFG
                     TIAPSGTQNVSVNFNTTGLSIGSYQGFLNIGSNDPLTPVRNVRIKLNVGEVGIQNNLT
                     GVPSDFMLDQNYPNPFNPSTKISYGIPKESFVSIKIYDILGKQIMSLVNENKRPGYYD
                     VNFNGSNFASGMYFYKIEAGSFVQTRRMILVK"
     gene            7577..11101
                     /locus_tag="PLX80_09300"
     CDS             7577..11101
                     /locus_tag="PLX80_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21023.1"
                     /translation="MRKLFMLIPVIVIGFFIMTGQKNPDAERWSNYLPSGIYDPSVRI
                     TATLPQVYDFTNPNTQISYVRTPYEVLAVSPNVRVLPRTNSYQSEVDIVRHPTNPLIM
                     FGSSNAFNNSGTLFISEGIYVTTDGGITWGGSDTMKLAGGAPVPNQGGDPGITIDKNG
                     TFIQTHLGFSTSGMFGNFSTDNGATWSSNFTIASGSQDKNLAGTDDSPTSPFYGRSYC
                     VWSLFTASAPPIDISYTTNGGVSWSSPQQINTPPAGHYSQGCDVKTGPNGEVYVVWAA
                     PIQGSPFTEDFAGFAKSTNGGVNWAVTENAYDMNGIRGTFPSKSNIRVNGFPRIDVDK
                     SGGARNGWIYTVTSEKNLAPAGTDPDIIMHRSSNGGANWSAGIRVNQDPLNNGKFQYF
                     PAIRVDEAGGVNIVYYDDRNTASDSSEIYISRSLDGGNTWSDVLVSDHRFKPQSIGVS
                     GIATGYQGDYIGMTSSNGKLWPLWMDNFSGIYNAWTAPVQITTFPLSSFNLTSPAAGT
                     TIATLPNSTTQYNFGWDTSASTASYKWIFGSPSASSRQITLPPSGNSLTVTGGQLDVI
                     LQGLGLNQGDSLVGSWDVWAFRNNQTNDSMKATNGPRTITLKRARPSLTAFNLISPPN
                     NTSLLTLVTNTTPVNINWTKSGQAVNYRWLYASPNFSNQANVKLRIAANNSGFDSLLT
                     LRNSQVDSALAGLGVNVGDSTVGQWRVYGYSGSDSLASAQTYNITMRRGIPPTITSSA
                     DSIVVSLPVGGSASRNLIIGNTGQFPLDWVITESSSSLDNLNVITTKIDEALILQQAN
                     LPKGADDIYHGPDVTDGMGGPDAFGYRWIDSDEPGGPVFNWYEISAIGTQITTWTSGT
                     GDDGSVIVPLPFGFSFYGTSYNQLKICTNGWVGFDVASISNAYLNVAIPATSEPNNAI
                     YPFWDDHDVRTSGQIFYHNDAANNRFVIEYKDVPHFTSGGPYTYQVMLYSDGRIIFQY
                     LNMTDPLNSATIGLENSTGTVALQVAFNAAYMHNNLAIKLEKGLAWVDETPSFGTIAP
                     SGTQNVSVNFNTTGLSIGSYQGFLNIGSNDPLTPVRNVRIKLNVGEVGIQNNLTGVPS
                     DFMLDQNYPNPFNPSTKISYGIPKESFVSIKIYDILGKQIMSLVNENKRPGYYDVNFN
                     GSNFASGMYFYKIEAGSFVQTRRMILVK"
BASE COUNT         3634 a         2084 c         2183 g         3503 t
ORIGIN      
        1 tgcgtctttg tgtctttgtg gtattaatta aaattatgtt ttgagacact ttcgaagaaa
       61 ggagagggag atctctatta aaagaatatc ggtatgagaa taccctagtc agatcttgaa
      121 aagtatgtac ctattattta ctaactatta actactaact attaactgtt taattgatct
      181 taactttttt cacatcacct atcggctgcc cgaggaatct ttcacttata tctttaaatt
      241 tattctgaaa gtcagtcaca taaaatttat gcacaccttt tttcttttga tcattgagta
      301 aatgtttttg accaagtata atttttactt cctttgcagt ctcttctcct gagtctatga
      361 gtattatctt ttttcccaaa acttcagata taactttttt tagaattgga taatgggtac
      421 acccgagtat caatgtatcg atgtttagct ttttaaattc atcgaggtat tcttttgcaa
      481 cgagttttgc gatcttgtta tcattccatc cgtcttcagt aagctgtaca aacaggctgc
      541 atgcttttga gaaaacttta atattgcttt caaatctgtg tatctgggtt ttatatgaat
      601 cactcattat agtaccaaga gtacctatca cacctattct gaaattttta gtctctgaca
      661 aagctgcttt gcatcccggt ttgatcacgc cgagtaccgg aatgtccgta agttttttaa
      721 gagaaggaag tgcaaatgag gttgcggtgt tacatgctac gactatcatt ttaacatttt
      781 tattcaaaag aaattttgta atttccatag aatattttat tactgtctct ttggactttg
      841 atccgtatgg taatctcgca gtatctccga gataaattat attttcattt ggaagtattt
      901 caaagagaga ccctgctaca gtaagacctc cgatgcctga atcaaataca cctatcggac
      961 ttcgtttgga tattttatta ttcaaatagt attaattgaa ttctattttt acaaatggaa
     1021 tgttttagca aattaaacaa tttaaacgtt tcattaaatg taacactttt ttccaattct
     1081 attttacaat acatgagatc ttttttcact aatttatttt tttgcttttc attatttttt
     1141 ataccttctc tcacccagtc tcaatggcag tggcagaatc ccattcctca gggaaatatt
     1201 cttttcggat ctcattttat aaatgacaat accggttatg catgcggaga cggcgggaca
     1261 ataatcaaaa caaccgacgg cgggaacacc tgggcaataa ataaaactaa aacaaaagaa
     1321 agtatccgtt cactttcttt cactgatgag aattcaggct gggcttgcgg acttgccggt
     1381 acgatattaa agacaacaaa tggcggagtt ttctgggata gaattatttc cggtactact
     1441 ggaaatttat tctgtgtaag atttttaaat tcaaacaccg gcataattgt cggcgatacc
     1501 ggtatagtta tgaaaactac taacggcgga aattcattcg aaagaaaacc tacaggtata
     1561 tcactttctc ttacaagctg ttcactgatg ggaaatattt ttttggctgc cggactgaac
     1621 ggagcatatg caaggtctgc tgactatggg aatacctggc agcagataag caccggaaat
     1681 aataattact ttttcgatat aatgtttaaa gattcagtca cttcttttat cagcggagct
     1741 tatggtacaa ttttacgcag tacagatgca ggtttgagct ggacacgaat gtccactctt
     1801 accggcaatt ggttttatac aatttcattt ggtgatgcaa tgaccggctt tgcatcgagt
     1861 gattacggta atgtttataa aacaactaat gcaggactta actggacagg actggcttct
     1921 actacttcag aagctcaata ttccctgaaa gcatttgacg aggatgaaat tataagcttt
     1981 ggaaataaag gcagtattat taccacttca aattcaggac ttaactggtc aaatataaca
     2041 tccggattca ggaacacaat ttattcgctg gatttcatta atgacaatac aggctatgga
     2101 tgcggcgggc aaggcagttt tatcaaaaca actaatggag gaacagactg gatccgttcg
     2161 caggcaggca gtacaaatga ttttcttttt aatatcgata tgacaaatga attcacagga
     2221 tattgtgcag gctacctggg gaaatttctc aagacaactg acggcggagc aaactggtca
     2281 gctgtaaatc ttaatactga aagatcaatc aatgcagttt atttttttaa tgaaaacacc
     2341 ggatggatat gcggtgattc cggaatgctt ttaagatcag atgatgggtt cgcttcttct
     2401 gtatctgtgc ccgctgatca gaattttgat attacagata tagaatttgt gaattctaat
     2461 gtaggatatt attcttcaca gcaaagaaag atatttaaaa ctacagatgc tggagagaac
     2521 tggttcacac cacatacagg tgattttgga agtactgtcg gcggactgga ttttattgat
     2581 gcaaatacgg gaattgcttt tacttataat aataaaattt taattacaaa tgacggcgga
     2641 gtaaactgga ttgagaaatt aatccctccc ggaaattatc ttaacaacgt ttacatttca
     2701 ggtcataata attactgggc tgtcggtgat tttggtttca tctacagatc cacagacggc
     2761 ggagcgtttt ggtatgaaga gaatgacaat aattttatgg accggatctt cggtatttat
     2821 tttaaaaata attctgaagg ctgggtttca ggcggaaacg gattgatagc tgaatattat
     2881 aatcctcaga gcagtattat ctcattaaat caaaacaata atactacagg ttacaaactt
     2941 aatcaaaatt atcctaaccc attcaaccct gctacagtca tacaatatga gctgcctgtt
     3001 tcaggaaata ttagtctgaa agtgtatgac gtacttggaa atgaagttgc ttaccttgta
     3061 aatgaaaggc agaatgcggg ttcacattcg gtaaaatttg acggaaacaa tttatcaagc
     3121 ggagtatatt tttacaaatt agaaaccgat ggtttttccg atacaaggag aatggtctta
     3181 ataaaatgag aagataattt ataccatttt atttaaacta ataaccgtta ttttaaaata
     3241 atttttttta tacacaaatc aaataattta acactcgaaa ggaaattaat gaagaagtta
     3301 tttctcattt tacctgttgc attaatttgt tttattttaa tgacaggcca aaattcgact
     3361 gaagataatc cgagatggag tattgatccg cggtcttcag taacttattc caacggagat
     3421 tataccagtc tcccgactac ggatattgga ataaagcaat tcacttttgc tccgcgtacg
     3481 atcataactc caattggcgt aatggttgct tcaccaaacg tgagagttca tccaagtgca
     3541 ggcactcagc agaacgaagt tgacattgtc agacatccga caaatccaaa cattatgttt
     3601 gctgctgcac agccgatcaa caacgctaac aatttcatca acgtcggtgt gtatgtaact
     3661 actgacggcg gtctttcctg gaacggaagg gatacaatga atgctccgaa tcttaatgat
     3721 cagcggggtg accccgctcc tattatagat aagaacggta catttttata tggtcacctt
     3781 acaagttctg caaatttcgg aggacttaca ggaatgggcg caaacttttc tacagataac
     3841 ggagctacct ggtcatcgac ttttcaagtt atggctaacg caaatgttga caaaaacatg
     3901 accggtactg acggttcgcc ttcaagtcct ttttatggaa acagttatat ggcatggact
     3961 gtatttgcag gaactgccgg taacggaaga gtttccagaa ctacaaatgg cggtgtctcc
     4021 tgggatccgg ttattacttt aaattcaaca cctgcaaatc attttgctca gggacatgac
     4081 gttgcagtag caccaaacgg aaatgttttt gtgtgctgga cagcaggaag ttctacctct
     4141 ccatttactg aagattttgt aggaattgca aaatccacag acggaggact tacttatact
     4201 gctactgaaa atgcttatga tgtaaacgga agcagatctg cttcattcaa tggctgggga
     4261 atcagaacca atggttttcc gagaattgac attgacaaaa gcggcggagc aagaaacggc
     4321 tggatctaca tagtagacgg acagatcaat cttgcaccgg caggatctga tgctgatgtc
     4381 attatgcgca gatcatctga taatggtgct acctggtcag caggtatcag agttaatcag
     4441 gatgcattaa ataacggaaa agtccagttc tttcctgtta taaatgttga tgacgccgga
     4501 ggactaaacg ttgtgtatta tgataacaga gattttccat cagtaaatga ttcctgttca
     4561 gtctatatgt caagatcgat agatggcgga aatacatggg cagatgttga agttgcagat
     4621 catcacttca aaccaaagaa tctacccggt atcaatacta tgggagatta tatcggcgtg
     4681 acttcaggta acggaaaagt ctggccggtt tggatggatg ataaagcagg tccgggagta
     4741 cagtttaaca tctggactac taatattcag atctcaactt tcccgctgag ttcattcaat
     4801 ttaacttcgc ctgcagcggg cacaacgatt gcaacattac caaacagtac tactcaatat
     4861 aattttggat gggatacatc tgcttcaact gcttcataca agtggatatt cggaagtcca
     4921 agtgcatctt caagacagat cactttaccg ccaagcggaa attcacttac agttaccggc
     4981 ggacagcttg atgtaatcct tcagggactt ggacttaatc agggtgactc gcttgtaggt
     5041 tcatgggatg tatgggcttt cagaaataat cagactaatg attcaatgaa agctacaaac
     5101 ggtcctagaa ctataactct gaaaagagcc agaccttctc ttactgcttt caatttaatt
     5161 tcacctccaa acaatacatc attattaaca ctggttacaa atacaactcc tgtaaatatc
     5221 aactggacaa aatcaggaca ggctgtaaat tacagatggt tatatgcttc accaaatttc
     5281 agcaatcagg caaatgtaaa attaagaatc gcagccaata acagcggatt tgattctttg
     5341 ctgacattaa gaaattcgca ggtggattct gcacttgctg gattaggtgt taatgtagga
     5401 gactcaacag tcggtcagtg gagagtttac ggttacagcg gaagtgattc acttgcttca
     5461 gcacagacat ataatattac tatgagaaga ggaattcctc cgaccattac ttcttcggct
     5521 gactcaattg tagtgagctt acctgtaggc ggatcagcat caagaaatct tatcattggt
     5581 aacaccggtc agtttcctct tgactgggta attactgaat cttcatcttc acttgacaat
     5641 ctgaatgtaa ttactactaa aatagatgag gctttaattc ttcagcaggc aaatcttcca
     5701 aaaggtgcag atgacattta tcacggacct gatgtgacag acggaatggg cggacctgat
     5761 gcatttggtt acagatggat cgacagtgat gagccgggcg gacctgtctt taactggtac
     5821 gaaatttcag caatcggtac acagatcact acatggacaa gtggtacagg tgatgacgga
     5881 tctgttattg ttccgcttcc attcggattc agtttttatg gaacaagtta caatcagctt
     5941 aagatctgta ctaacggatg ggtaggattt gacgtagcca gtatatcgaa tgcatacctc
     6001 aatgttgcaa ttcctgcaac atcagaaccg aacaacgcga tctatccttt ctgggatgat
     6061 catgatgtaa gaacttcagg tcagattttt tatcataacg atgcagcaaa caacagattt
     6121 gttattgagt ataaagacgt tcctcatttt acctcaggcg gaccttatac ttatcaggta
     6181 atgctttatt cagacggaag aattattttc cagtatctga acatgacaga tccattgaat
     6241 tcagcaacta tcggtcttga aaattccact ggtacagtag cattacaggt agcattcaat
     6301 gcagcctaca tgcataataa tcttgctatc aaacttgaaa aaggacttgc ctgggttgat
     6361 gaaacacctt cattcggaac tatcgctcct tcaggaactc agaatgtcag cgttaatttc
     6421 aatacaaccg gactaagcat tggttcttac cagggattct taaatatcgg aagtaatgat
     6481 cctttaacac cggtaagaaa tgtcagaatt aaattaaatg tcggtgaagt aggtattcaa
     6541 aataatctaa ccggtgtacc gtcagatttt atgctggatc agaattatcc gaatcctttc
     6601 aatccatcta caaagataag ttacggaatt ccgaaggagt catttgtttc aatcaagatc
     6661 tatgacatac tcggcaagca gataatgagt cttgtaaatg aaaataaacg acccggatat
     6721 tatgacgtga attttaacgg atcaaacttt gcaagcggaa tgtacttcta taagatagag
     6781 gcaggaagct ttgttcagac aagacgtatg atactcgtta aataatttca aattacaaat
     6841 tacaaattac gaatacgtaa tttgtttatt cttcagtccc gcagagatca gattttctct
     6901 gcgggatttt tttatgtctt tgcgaatccg aatttattcg ggtgaagcaa tctgaatttt
     6961 aatctgtttt aaaattcaac ggattactta tttataaaaa aaattaactg ataatttata
     7021 ccattttatt taaaatcata aaagttattt taaataaatt tttttattat caaacgatat
     7081 aatttttaaa ctgaaaggaa ataaataaag aagtctttct tcattttaca tccagtataa
     7141 atgtgattaa tactattgac tgctaaacat tctaattaaa ataatcagat gtagagtttt
     7201 gatccactta cttcagtaac ttattccaaa atggattaca acaatccgga attatgtttg
     7261 tttttgaaca gccgaattta aatacaataa atttttttaa agacgacatg tttgtcaata
     7321 ctaaaaaata atgaaaaaat tacaacctac attcaaaagg gatttattaa ttttatgaaa
     7381 tcatagttag ttaaatatta ataaaattaa taaaattaat aaaattagtt tcagcaaatt
     7441 ttaaaattac atgtacaaat ttacaaatgc gtaaattctc agaattccaa acatttttta
     7501 taatacttcc agtattatta ctcagatttt tacaaacact attttataat catttaacag
     7561 aaaaggactc ctttttatga gaaagttatt catgttaatt ccggtcatag taattggatt
     7621 ttttattatg acaggacaaa aaaatcctga tgctgaaaga tggagcaatt atcttccttc
     7681 aggtatttat gatccaagtg tgaggattac tgcaacattg cctcaggtat atgatttcac
     7741 aaatcctaat actcaaattt cgtatgtaag aactccatat gaagttcttg cagtctcacc
     7801 aaacgtcaga gtattgccaa ggactaacag ttatcaaagt gaagttgata ttgtaagaca
     7861 tccgactaat cctttgataa tgtttggttc ttcaaatgca tttaacaatt caggaacact
     7921 gtttatcagt gaaggtattt atgtgacaac tgacggtggt attacctggg gtggatcaga
     7981 tacaatgaaa ttagccggcg gtgcaccggt accaaatcag ggtggtgacc cgggtattac
     8041 aattgataaa aacggaacat ttattcaaac acatcttgga ttctcaactt caggaatgtt
     8101 tggaaatttt tctacagata acggagcaac atggtcatct aattttacaa ttgcttcagg
     8161 atcacaggat aaaaatttag caggtacaga tgattctccg accagtccat tttatggaag
     8221 atcatattgt gtatggtctt tatttacagc atcagctcct cctatagata tttcttatac
     8281 aacaaacgga ggtgtatcat ggtcatctcc tcagcagata aacactcctc ctgccggaca
     8341 ttattcacag ggatgtgatg tcaaaacagg tccaaacgga gaagtttatg tagtctgggc
     8401 tgctccaatt cagggatctc cttttacaga agactttgct ggttttgcaa aatcaactaa
     8461 tggcggagta aactgggcag taacagaaaa tgcatatgat atgaacggga tcagaggtac
     8521 attcccatca aaatcaaata tcagagttaa cggatttcca aggatcgacg ttgacaaaag
     8581 cggcggagca aggaacggct ggatatatac agttacttca gaaaaaaatt tagcacctgc
     8641 aggtacagat cctgacatca tcatgcacag atcatctaat ggcggagcaa attggtcagc
     8701 aggaatcaga gttaatcagg atccgcttaa taatggaaaa tttcaatatt tcccggctat
     8761 cagagtagat gaggcaggcg gagttaacat agtttattat gatgacagaa acactgcttc
     8821 tgattcttca gaaatttata tttcgagatc acttgacggc ggaaatacct ggtcagatgt
     8881 tttagtcagt gatcacagat tcaaaccaca gtcaataggc gtttccggaa ttgctacagg
     8941 atatcagggt gattatattg gaatgacttc aagtaacgga aaattatggc cattatggat
     9001 ggataacttt tcaggtatat acaatgcctg gactgcacct gttcagatca ctactttccc
     9061 tctgagttca ttcaatttaa cttcgcctgc agcaggcaca acgattgcaa cattaccaaa
     9121 cagtactact caatataatt ttggatggga tacatctgct tcaactgctt catacaagtg
     9181 gatattcgga agtccaagtg catcttcaag acagatcact ttaccgccaa gcggaaattc
     9241 acttacagtt accggcggac agcttgatgt aatccttcag ggacttggac ttaatcaggg
     9301 tgactcgctt gtaggttcat gggatgtatg ggctttcaga aataatcaga ctaatgattc
     9361 aatgaaagct acaaacggtc ctagaactat aactctgaaa agagccagac cttctcttac
     9421 tgctttcaat ttaatttcac ctccaaacaa tacatcatta ttaacactgg ttacaaatac
     9481 aactcctgta aatatcaact ggacaaaatc aggacaggct gtaaattaca gatggttata
     9541 tgcttcacca aatttcagca atcaggcaaa tgtaaaatta agaatcgcag ccaataacag
     9601 cggatttgat tctttgctga cattaagaaa ttcgcaggtg gattctgcac ttgctggatt
     9661 aggtgttaat gtaggagact caacagtcgg tcagtggaga gtttacggtt acagcggaag
     9721 tgattcactt gcttcagcac agacatataa tattactatg agaagaggaa ttcctccgac
     9781 cattacttct tcggctgact caattgtagt gagcttacct gtaggcggat cagcatcaag
     9841 aaatcttatc attggtaaca ccggtcagtt tcctcttgac tgggtaatta ctgaatcttc
     9901 atcttcactt gacaatctga atgtaattac tactaaaata gatgaggctt taattcttca
     9961 gcaggcaaat cttccaaaag gtgcagatga catttatcac ggacctgatg tgacagacgg
    10021 aatgggcgga cctgatgcat ttggttacag atggatcgac agtgatgagc cgggcggacc
    10081 tgtctttaac tggtacgaaa tttcagcaat cggtacacag atcactacat ggacaagtgg
    10141 tacaggtgat gacggatctg ttattgttcc gcttccattc ggattcagtt tttatggaac
    10201 aagttacaat cagcttaaga tctgtactaa cggatgggta ggatttgacg tagccagtat
    10261 atcgaatgca tacctcaatg ttgcaattcc tgcaacatca gaaccgaaca acgcgatcta
    10321 tcctttctgg gatgatcatg atgtaagaac ttcaggtcag attttttatc ataacgatgc
    10381 agcaaacaac agatttgtta ttgagtataa agacgttcct cattttacct caggcggacc
    10441 ttatacttat caggtaatgc tttattcaga cggaagaatt attttccagt atctgaacat
    10501 gacagatcca ttgaattcag caactatcgg tcttgaaaat tccactggta cagtagcatt
    10561 acaggtagca ttcaatgcag cctacatgca taataatctt gctatcaaac ttgaaaaagg
    10621 acttgcctgg gttgatgaaa caccttcatt cggaactatc gctccttcag gaactcagaa
    10681 tgtcagcgtt aatttcaata caaccggact aagcattggt tcttaccagg gattcttaaa
    10741 tatcggaagt aatgatcctt taacaccggt aagaaatgtc agaattaaat taaatgtcgg
    10801 tgaagtaggt attcaaaata atctaaccgg tgtaccgtca gattttatgc tggatcagaa
    10861 ttatccgaat cctttcaatc catctacaaa gataagttac ggaattccga aggagtcatt
    10921 tgtttcaatc aagatctatg acatactcgg caagcagata atgagtcttg taaatgaaaa
    10981 taaacgaccc ggatattatg acgtgaattt taacggatca aactttgcaa gcggaatgta
    11041 cttctataag atagaggcag gaagctttgt tcagacaaga cgtatgatac tcgttaaata
    11101 atttcaaatt acaaattaca aattacgaat acgtaatttg tttattcttc agtcccgcag
    11161 agatcagatt ttctctgcgg gatttttatt caattacaaa tttcaaatta ctaattgtaa
    11221 aaacaaaatt taaaaaaact actatcccgc agagatcaga ttttctctgc gggattgttt
    11281 tgtttaaatg ctcttctccc ctcctttctt ttcaaaggag gggacggggg gaggttaacg
    11341 aaaccgcatt ggaagtgcga aaaccaaaac ccaataaatt cattcttgta atgctctaaa
    11401 gatt
//
LOCUS       DAORVQ010000104        11127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_2979_length_11127_cov_5.903450, whole genome shotgun sequence.
ACCESSION   DAORVQ010000104 DAORVQ010000000
VERSION     DAORVQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_11127_cov_5.903450"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..2683)
                     /locus_tag="PLX80_09305"
     CDS             complement(218..2683)
                     /locus_tag="PLX80_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21024.1"
                     /translation="MKQLLFLLVMFSFCLTENSISQITNNDLPNSIAIQPPNMTMSPA
                     GTLSTPVTVNGFDNFNLGVDFGEPHIATNPRDPLNSICAFNINNFYYTLNGYNWIKIN
                     VTFPGYAVIGDPVMAFDSLGNAYYTQLYQNGGTYGVCISKSTNKGVTWTSTYSVYATN
                     VGLSDKEWIAADQTAGPYSNNIYACWRQFGANGMRFTRSIDQGATWSAPQTFSGGQGA
                     YVAVGPNGNVQGGSVYFAALNGGAAIVNRSTDGGVNFSPQTVASSYPTGGVFCAGRNT
                     VKDCIRMDGFPRMAADNSYTASRGYVYVVYARNPGTADVTDINMVRSTDFGVTWSAPV
                     RVNDDNTTTDQWMPAISVDSKTGRLYIAWYDSREDPTNNLLTKIYGTVSTDGGATFTA
                     NKPISNALFNPNNMGVNQPGGEKYIGDYFGIQAIGNTSYVVWMDGRNNQLGSFTGYYP
                     DYAMTVNKNYANVGNNDNTSIVVKVPAVNGPFVGGVKFSASLETPPASGNITFSFLNG
                     KDSVSTFPDSVTLIAATSGSVTPGGYVINIRGIGSQGPPVHVRTTTLLVNASLLTVGT
                     NRPGIANFTVNGTTYNQTQELIYPNGTNVNVTAVSPRTIGSTQYVFTNWSNGGDTTQT
                     INLSSNLNLSASYKVQYKLLTTSTQGTTFGGNVFYDSGTSATFAISSRIINNGGTVYY
                     FRGWTGSGSGSYTSPDSSGNDSAVTINVVAPLVESARWTTTVGITSLGSEVPAEYSLK
                     QNYPNPFNPSTVINFSIPKSGFVSIKVYDITGREVAVMLNEFKAAGNYSVDQNFTNFN
                     SGVYFYKIQAGEFTDIKKMTLLK"
     gene            complement(2813..4492)
                     /locus_tag="PLX80_09310"
     CDS             complement(2813..4492)
                     /locus_tag="PLX80_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HQY21025.1"
                     /translation="MKKIFLIVTFFILIFGKNANAQVEYVQVNSPVYHYLERMLTMKV
                     ITGYNPAVIPISRGAVADFLKVIIANTNKISSTDKQILADFKVEFQYELTGELKKSTS
                     FFNNTDHFLSDDYQKYLYSYSDSNATLFLDGLASLSGRGSKGDSIGRNSIGLGELGFR
                     VRGTLYDKVAYYLRASNGQKLWGDSSDVTFARNTDPKFYAQYKFQYEQRNFDTFDGYL
                     RYQTAGNWLALTAGRNPVYQGFGFIDRLYLSHNTVPFDFLKLDLAYKAVTYSFLYGSI
                     RGDSLGTPLDAKNIATHRLDVSFGNNFRMGLFESVIISNSPFSFTFLNPISFLTSAEL
                     SKASQTGDNNANNSILGLDAFVMPVKNLAFQGSFLVDDLEFSTLFKDNAQVTNKFAWQ
                     LGGLWSNAFTLPNLDMRIEYTLLEPYVYSHVSNKSTYTNWDLPLGHHLEPNSDEIAFR
                     FNYDFTNRINLNLMYQFQRSANGIVTDSAGRVLVNYGGNRNNATGYSYSDPEFLKGNR
                     INRSIITANFTWEFLRQFYIEFNYVHRAIDNIYQNVKFNDDYGFATIRIDY"
     gene            complement(4553..6478)
                     /locus_tag="PLX80_09315"
     CDS             complement(4553..6478)
                     /locus_tag="PLX80_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQY21026.1"
                     /translation="MLENFLNNKLLKLRNRHLLVLDILFLIISPFLALAVRLDGQIDF
                     TYYLHALTYSTLIFLISNLFIFYKLGLYKRYWNSASVDELAKIIYIVFFAILAETVIF
                     ILFFNLSWFNFERLPQSLPILDGIFKFIFVASARFSIRLFERLNERRSISYHGERVLV
                     VGSGKSGTSIVQEMQRNPQLGLNPVAFVDDDIEKKDLKIRGIPVMGTRNSIPEVITKM
                     DINKVIIAMPTALGSDIRDIVSICRNTGVQTRTIPGIYELLDDNIRIESIRQVQIEDL
                     LRREPVKTDIKKVEDFLKGKKVLVTGSGGSIGSELCRQIIKCNPSEIILLGQGENSIF
                     DINYELMKRISIVNGKQLLTKITTVIANIRSYSRLDKLFEEIKPDVIFHAAAHKHVPM
                     MESNSCEAISNNVKGTQNLIDLSLKHNVNHFVFISTDKAVNPSSIMGASKRVAEMIVL
                     NAAREHRKNYSTVRFGNVLGSRGSVVLTFKRQIAEGGPVTITDPDIQRYFMTIPEAVQ
                     LVLQASVIGNGGEIFVLDMGKPIKIIDLAKDIIRLSGYEVDKDIKIVETGLRPGEKLF
                     EELFIAGEIYEKTIHEKILIATNASHFIPENFKSNLELLLTKRYDNGSETIKYHLKLL
                     VPEYMSDEKIHTNVSIV"
     gene            complement(6656..7246)
                     /locus_tag="PLX80_09320"
     CDS             complement(6656..7246)
                     /locus_tag="PLX80_09320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQY21027.1"
                     /translation="MVKRLFDIIFSLIGLVFLAPFLILITIIIKADSKGPVFYIQKRV
                     GKGNKDFRLFKFRSMRIDSDISGLLTVGNKDPRITSSGYYLRKYKLDEIPQLYNVLKG
                     EMSFVGPRPEVRKYVELYNEYQMKVLEVKPGITDLASIKYRNENELLADSDDPEGLYT
                     NEIMPDKIEINLEYISDRSLLKDIRIIIKTLKAVII"
     gene            complement(7247..8461)
                     /locus_tag="PLX80_09325"
     CDS             complement(7247..8461)
                     /locus_tag="PLX80_09325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY21028.1"
                     /translation="MIPFSPPYIDEDVINEVLDSLNSGWITTGPKVKALEKEVAKLTG
                     VENVLCVNSATSAMMLVLHWYGIGPDDEVIIPAYTYCATALAVMHLGATPVMADCDEN
                     FCIDVSKLEKLINEKTKAIVPVDIGGYPCDYEAINKLISEPEVKKLFAAKGEVQNKLG
                     RILVMSDAAHSIGACYKGNESGSLCDVTVFSFHAVKNVTTAEGGAICLNLPIPFDNTE
                     LYSWLRLLSLNGQTKDAFTKSLGGNWKYDIVYPGFKINLSDLCAAVGLAQIRKYTADL
                     LLKRKEIFRKYNEAFSKTNWAELPPSDSEGRESSYHIYLLRIKKISEDQRDSIIDEIN
                     IKQVAVNVHFIPLPLLKIFKDRNFDIKNFPVSYDNYSRVITLPVYPQLTDENVEKIIS
                     AVVESYNSVVNK"
     gene            complement(8458..10062)
                     /locus_tag="PLX80_09330"
     CDS             complement(8458..10062)
                     /locus_tag="PLX80_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21029.1"
                     /translation="MNSVRNFLFNGRNSFLKGTGIFLVFFFITRLPYLLYYRVDILSN
                     DSASYIAAALSILNSVMPLFDIRTPGYPLFLAMIWSFSKSIFAVSVMQSAFSLFTGVI
                     IVYLFDKIYKKFTVVFACFLTVYMTSSYFLLFESALLTEGIFTNMLLICTALMMISIK
                     ENKNKYWILFSISVAILILIRPAGLFFVGIMVLIILYFIINRYSLIKYVLLIIPFSVI
                     IISLCTYNYITLNKFTITPFGEANLSGVTILFMETSDEYPGFVNSAIQQTLDSIPKKD
                     KSYIRNYNGLTKLFNVFKDNFHRQMNFAGNLMGDDSTKKYMDVQPYMRIVSVDAIKKN
                     PFIYLKFFSCNFFYFFKNLGIEMKYFNQLMYNYNKNVVREMFVKELEKKKWLQISSDR
                     SVNDQVKDFLVEEIIQQKELKYIVEDQNNEAVFENTLLKSVYDYYESFYNFFLRNIVW
                     YIGFFTMFMLSIIILFKTKFSDPDAIIAFLIFMIFLSKALLVSMVESSLERYSYTVEF
                     GVYFSIPFLLILIQNLKNMKLKNKNI"
     gene            complement(10059..10943)
                     /locus_tag="PLX80_09335"
     CDS             complement(10059..10943)
                     /locus_tag="PLX80_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21030.1"
                     /translation="MISVVIPAYNEGSVIAMTVFDIKKVAELNNLRDIEIIVVDDGST
                     DRTAEMSEKAGAVTIRHPHNIGYGKSLKDGIEAATNDIIVITDADGTYPLEMIPVLVS
                     EYNKGFDMVVGARQGENYDESFKKKVLRLILKKLVEFSAGRKIDDINSGLRVFSKSKV
                     LPYFSKLCNTFSFTTSLTLAYMMNAMYVKYIPIDYKKREGKTKVKLFRDSMRTLQFIT
                     EAILFYNPIKIFIVFCLALLAFAGVNFIFSIIFRMPITFYLGLGSILLSILMFGMGLI
                     SVQLKHLMVSDDVKNRLK"
BASE COUNT         3674 a         2033 c         1776 g         3644 t
ORIGIN      
        1 tttacaattt ctcaagaatt cttaacggca atgtattaca ggcgggattt ttttttataa
       61 aatacttcta acgttaataa aaaaaatatt ccttcaagaa tattaggggt gtccagatgc
      121 gtaaaacagc aaagatttcc ccaaataaaa aacgccggag tctcaagtat ggcagagaga
      181 caccggcgtt aaaattaaga tctgtatctt tttacattta tttcagcaag gtcattttct
      241 taatgtctgt aaactcacct gcctggattt tataaaaata aaccccgctg ttgaaatttg
      301 tgaaattctg atccacactg taatttcctg cggctttaaa ctcattgagc attacagcga
      361 cttccctacc tgtaatatca tagaccttaa ttgaaacaaa tccgcttttg ggaattgaga
      421 aatttattac agtcgatgga ttaaatggat tcggataatt ttgctttagt gaatattcag
      481 caggaacttc tgagccgagt gaagtaatgc cgactgtcgt agtccatctt gctgattcta
      541 caagtggtgc tactacattt atcgtgactg ccgaatcatt acccgaactg tctggacttg
      601 tatatgagcc cgaccctgac cctgtccatc ctctgaaata ataaactgtc ccgccgttat
      661 taataattct tgagctaatt gcaaaagtag cgctggttcc tgaatcataa aaaacatttc
      721 caccaaaagt agtaccctgt gtagaggtgg ttaaaagttt atactgcact ttataagatg
      781 ctgacagatt cagattagaa cttaaattta tagtctgagt agtatcgccg ccgtttgacc
      841 agtttgtaaa tacatattga gtagagccga tagtcctcgg actgactgca gtaacattta
      901 catttgttcc gtttggataa ataagctctt gtgtctgatt ataagtagtt ccgttcacag
      961 taaagtttgc aatgcccggt ctgttagttc cgactgtcag cagtgatgca tttaccagaa
     1021 gagttgtagt tcttacatga acaggaggtc cctgcgaacc aatacctctt atattaatta
     1081 cataacctcc cggagttaca ctgcctgaag tagctgcgat cagcgttact gagtcaggga
     1141 aagttgaaac agaatcttta ccatttaaaa atgaaaaagt aatatttccg gaagcaggag
     1201 gagtttcaag agatgctgaa aatttcacac cgccaacgaa aggaccgttg acagctggta
     1261 ctttcacgac tattgaagta ttgtcattat ttccgacatt agcataattt ttatttacag
     1321 tcattgcata atccggataa tagcctgtaa aacttccaag ctgattattt cttccgtcca
     1381 tccatactac ataagaagta tttccgattg cctgtattcc aaaataatcg ccgatatatt
     1441 tttcaccgcc gggctgattt acacccatgt tatttggatt gaagagagca ttagagatcg
     1501 gcttattagc tgtaaaagtt gcaccgccgt cagttgacac tgtaccgtaa atttttgtca
     1561 gaagattatt cgttggatct tctcgtgaat cataccatgc aatatataat ctaccggtct
     1621 tactgtccac agagattgca ggcatccact ggtccgtagt tgtgttgtca tcattgactc
     1681 ttacaggtgc tgaccatgtc actccgaaat cagtagatct caccatatta atatcagtca
     1741 catctgctgt accaggattt ctggcatata caacatatac atatcctctt gatgcagtgt
     1801 agctgttatc tgcagccatt ctcgggaacc cgtccattct gatacaatct ttaacagtat
     1861 tccttccggc acagaaaact ccgccggtag gataagaaga agcaactgtt tgaggtgaaa
     1921 aattcacacc accatcagtc gatctgttaa cgattgcagc tccgccattc aatgcagcaa
     1981 agtaaacgct tccaccctga acatttccat ttggtccaac agcaacgtaa gctccctgac
     2041 cacctgaaaa tgtctgaggt gcactccatg ttgcaccctg atcgatgctt cttgtgaatc
     2101 tcattccatt tgcaccgaat tgtctccagc atgcataaat attatttgaa taaggaccgg
     2161 cagtctgatc tgcagcaatc cattctttat ctgaaagacc aacattcgtt gcatagacac
     2221 tgtaagtaga agtccatgtg acacctttgt ttgtagattt tgaaatacaa actccatatg
     2281 ttccgccatt ctgataaagc tgtgtatagt aagcatttcc cagactgtca aatgccatta
     2341 caggatcacc aattactgca tatccgggga acgttacatt tatttttatc cagttgtatc
     2401 cattcagtgt ataataaaaa ttgttaatgt taaatgcgca gatgctgttc agtgggtctc
     2461 tcggattagt ggcaatatgc ggttcaccaa aatcaacacc gaggttgaaa ttgtcaaaac
     2521 cgtttacagt tacaggtgta cttaaagtcc ctgctggtga cattgtcata ttcggcggct
     2581 gtatagcaat cgaatttggc agatcattat ttgttatctg cgaaatggaa ttttctgtca
     2641 gacagaaact aaacatgacc agaagaaata gtaattgttt catttattgt ttatgatttt
     2701 taaaaaaaat ttttataaaa taattgttaa tggcagtaaa atcatcttta tttaaaataa
     2761 tcttcgatcc tgaaataaat tacttctatt aatgaaaaag taattgaatg ctttagtaat
     2821 caattcgaat agttgcaaag ccgtagtcat cattaaattt cacattctga taaatattat
     2881 caatagctct gtgtacataa ttgaattcga tataaaattg tctcaggaat tcccatgtaa
     2941 aatttgcagt gatgatactt cggttgatcc tgtttccttt aagaaactcc ggatctgaat
     3001 atgaataacc tgtggcatta tttctgttgc cgccatagtt tactaatacc ctgccggctg
     3061 aatcagtgac tataccgttt gcagatctct gaaactgata cataagattc aaatttattc
     3121 tgttagtaaa atcataatta aatctgaatg cgatctcatc tgagtttggc tcaaggtgat
     3181 gtccgagcgg aaggtcccag tttgtataag ttgatttatt tgacacatgc gaatatacat
     3241 aaggctcaag taaagtatat tcaattctca tatcaagatt tggaagtgtg aaagcattcg
     3301 accaaagtcc tccgagctgc catgcgaatt tatttgtgac ctgtgcatta tctttaaaca
     3361 atgtactgaa ctcaagatca tctacaagaa atgaaccctg aaaagctaaa ttcttaaccg
     3421 gcatcacaaa agcatcaagt ccgagtattg aattatttgc attattatct ccggtctgtg
     3481 aagctttgct gagttcggca gatgtgagaa aactgattgg atttaaaaaa gtaaaactaa
     3541 acggagaatt tgaaatgatc acactttcaa ataatcccat tctaaaatta tttccaaaag
     3601 aaacatcaag tctgtgcgtt gcaatatttt ttgcgtcaag cggagtaccg agtgaatccc
     3661 ctcttatgct tccatataaa aaagaataag taacagcttt gtatgcaaga tcaagtttaa
     3721 gaaaatcaaa tggaacagta ttatgagaaa gatataatct gtcaataaat ccaaaaccct
     3781 gatagacagg atttctaccg gcagtaagag ctagccaatt tccggcagtc tgatatctga
     3841 gataaccgtc gaaggtgtca aaatttcttt gctcatattg aaacttatac tgcgcataaa
     3901 atttcggatc tgtgtttctt gcaaaggtaa catctgaaga atcgccccat agtttctgtc
     3961 cgtttgaagc tctgagataa tatgcgactt tatcatacaa agtacctctt actctgaaac
     4021 cgagttcacc aagtccaatg gaatttcttc cgatagaatc tcctttggaa cctctccctg
     4081 acaatgatgc gagtccgtca agaaacaatg tagcattcga atctgagtat gaatataaat
     4141 atttctgata atcatctgac aagaaatgat cagtattatt aaaaaaggaa gttgattttt
     4201 tcagctcacc tgtaagttca tattgaaatt cgactttgaa atctgcgagt atttgtttgt
     4261 ctgtagaaga aattttattt gtatttgcaa taatgacttt aagaaaatca gcaacagctc
     4321 ctctcgagat tggaatcaca gccggattgt aacctgtgat aactttcatg gtaagcattc
     4381 tctcgaggta atgataaacg gggctgttta cctgcacata ttctacctga gcatttgcat
     4441 tttttccgaa gatcagaata aaaaaagtaa caatgaggaa aatttttttc ataaagaaaa
     4501 aattatttat taatgatata tattactaaa tttattttca gaaagaaaag cgtcagacaa
     4561 tactgacatt ggtatgaatt ttttcatctg acatatattc ggggacaaga agtttcagat
     4621 gatacttaat ggtttcgctg ccgttatcat atctttttgt caatagaagt tcaagattag
     4681 atttgaaatt ttcgggaata aaatgactgg cattagttgc aatcagaatc ttttcgtgaa
     4741 tagttttttc atatatctcg cctgcaataa acagttcttc aaacaatttc tctcctggtc
     4801 tgagtccggt ttcgactatt ttaatatctt tgtctacttc ataaccggaa agtctgatta
     4861 tatcttttgc aaggtcaatg atctttattg gcttacccat atcgagtacg aatatttctc
     4921 cgccatttcc aattactgat gcctgtaata ctaattgcac agcttcggga atagtcataa
     4981 aatatctttg aatatccgga tctgtaattg taaccggtcc gccttcagcg atctgtcttt
     5041 taaaagtaag aacaacactg cctctgctgc caagtacatt tccaaatctg acagtagaat
     5101 aatttttcct gtgctctctt gcagcgttaa gaactatcat ttcagcgaca cgtttgctgg
     5161 cacccattat actcgaagga tttacagctt tatcagtgga tatgaaaaca aagtgattta
     5221 cattatgttt taaagaaaga tctatcaggt tctgagttcc tttgacatta ttgctgatag
     5281 cttcacatga gttagattcc atcattggaa catgcttgtg agcagcagca tgaaaaatca
     5341 catcgggttt aatttcttca aaaagtttat ctaatctgct gtacgatctg atattggcaa
     5401 taacggttgt aatttttgtt agtaattgtt ttccgttgac aatagagatc cttttcatta
     5461 attcataatt gatatcaaaa atagaattct caccctgacc aaggaggata atttcagatg
     5521 gattacattt tataatctgt ctgcatagtt cacttccaat agatccaccg gatcctgtaa
     5581 caagtacttt tttaccttta agaaaatctt caactttttt aatatcagtc tttacaggtt
     5641 ctcttctgag caggtcttcg atctgaacct gtcttatact ttctattctg atattgtcat
     5701 cgagtaattc atagataccg ggaatagttc tggtctgcac gcctgtattt ctgcagattg
     5761 aaactatatc tcttatatca gagcccaaag cagttggcat tgcaatgata actttattga
     5821 tatccatttt ggtaatgact tcaggaattg aattccttgt acccatcacc ggaatacctc
     5881 tgatcttaag atcttttttc tcaatatcat catcaacaaa agctacggga ttcaatccaa
     5941 gctgtggatt tctctgcatt tcctgtacaa tggaagtacc ggattttcct gaacctacta
     6001 caagtacacg ttcaccatga tatgagatac ttcttctttc attaagtctt tcaaataatc
     6061 tgatactgaa tctcgcactg gcaacgaaaa taaatttaaa tattccgtca aggattggta
     6121 aagactgcgg taatctttca aaattaaacc aggaaagatt aaagaataaa ataaaaatta
     6181 ctgtctcagc taaaatagca aagaaaacga tataaattat tttagctaat tcatccacac
     6241 tcgcactgtt ccagtatctt ttgtacagac cgagtttata aaatataaat aagtttgaaa
     6301 ttaaaaatat taaagtggag taagtaagag catgcaggta ataagtgaag tcaatttgcc
     6361 cgtcaagtct tacagcaaga gctaaaaatg ggctaattat aaggaaaaga atatctagga
     6421 caagtaaatg cctgttccga agtttgagga gtttattatt taaaaagttt tctaacataa
     6481 attaattaag tctctccgtt caaataatct ccttggggaa attttcataa gttcaaataa
     6541 gaacaaactt atcaggggta acaatttagt tttgataatc agttggacaa agttaattaa
     6601 atcatttaac tgattcaatt aatttcttta tattattttg taaataatat aaatatcata
     6661 ttattacagc tttcagggtt ttaataatta ttctgatatc tttgagaaga gatctgtcag
     6721 aaatatattc aagattgatc tctattttat ccggcataat ctcatttgta tacaatcctt
     6781 ccggatcatc agaatctgca agaagttcat tttcatttcg gtatttaata gaggcaagat
     6841 ctgtaatgcc aggttttact tcgaggactt tcatctgata ttcattataa agttctacat
     6901 atttccgtac ttcaggtctt ggtcctacaa aactcatttc acctttcaat acattgtaaa
     6961 gctgaggaat ttcatcgagt ttgtatttcc gaagatagta tcctgaagaa gttatgcgcg
     7021 gatctttatt tccaacagtt agcaatcccg atatatcgga atcaattctc attgatctga
     7081 atttgaataa cctaaaatct ttatttccct ttcccactct tttttggata taaaatacag
     7141 gaccttttga gtctgctttt attattatag ttataagaat taaaaaagga gcaaggaaca
     7201 ctaatccgat taatgaaaaa ataatatcaa ataatctttt aaccaattat ttatttacaa
     7261 ctgaattgta agattcaaca acagcactta tgattttttc aacattctca tcagtaagct
     7321 gcggatatac aggcagggtt attactcttg aataattgtc ataacttact ggaaaatttt
     7381 tgatatcgaa atttctatcc ttaaatattt tcagcaaagg caacggaatg aaatgaacat
     7441 ttactgcaac ttgctttata ttaatttcat ctataattga atcacgctga tcttctgaaa
     7501 tttttttaat tcttaataaa taaatatgat aagaagattc tctgccttca gaatctgaag
     7561 gaggcagttc tgcccagtta gttttcgaaa acgcttcatt atattttctg aagatctctt
     7621 ttctttttaa taaaagatct gccgtatatt ttcttatttg tgcaagccct acagcagcgc
     7681 aaagatcgct tagattaatt ttaaatccag gatatacaat atcatatttc cagttgcctc
     7741 cgagagattt tgtaaaagca tcttttgtct gcccgttcag agaaaggagt cttaaccagc
     7801 tatacaactc agtattatca aaaggtattg gcaaattcag acagattgcc ccgccttcag
     7861 cagtcgtaac atttttcaca gcgtgaaatg aaaacacagt tacatcacaa agtgaaccac
     7921 tttcatttcc tttataacat gcaccaatag aatgagctgc atctgacatt actaaaattc
     7981 ttccaagctt attctgtact tcaccttttg cagcaaataa tttttttact tcaggttctg
     8041 agattaactt atttattgct tcatagtcac agggatatcc tccaatatca acaggtacta
     8101 ttgctttggt tttctcatta attaattttt ccaatttaga aacatcgata cagaaatttt
     8161 catcacagtc agccataacc ggtgtcgcac cgagatgcat aacagcaagt gcagtcgcgc
     8221 aataagtata agccggaata atcacttcgt catcaggacc tataccatac cagtgcaaaa
     8281 ccagcatcat agcagaagta gccgaattta cacacagtac attttcaaca ccggtaagtt
     8341 ttgcaacttc tttttcgaga gcttttactt ttggacctgt agtgatccaa cctgaattta
     8401 acgaatcaag aacttcatta attacatcct catctatata aggaggcgaa aacggaatca
     8461 tatattttta ttttttaatt tcatgttttt taaattctga attaatatta ataagaatgg
     8521 aatgctgaaa taaactccaa actccacagt atagctgtat ctctcaagtg acgattcgac
     8581 catactaact aaaagtgctt ttgataaaaa gatcataaag attagaaatg caataattgc
     8641 atcagggtct gaaaatttag ttttaaaaag aattataata cttaacataa acattgtgaa
     8701 aaatccgata taccatacaa tatttcttaa aaagaaatta taaaaactct cataataatc
     8761 ataaacggat ttcaataatg tattttcaaa aacagcttca ttattctggt cttcaacaat
     8821 atattttaat tccttttgct gaataatctc ttctacaaga aagtccttaa cctgatcatt
     8881 tactgatctg tctgaagata tctgaagcca tttctttttt tcaagttctt taacaaacat
     8941 ttcacggact acatttttat tgtaattata cattaactgg ttaaaatatt tcatttcaat
     9001 gccaaggttt ttaaaaaaat aaaaaaagtt acaggaaaaa aatttcagat atataaaagg
     9061 atttttttta atcgcatcaa cagaaacaat tctcatgtac ggctgtacat ccatatactt
     9121 ttttgttgaa tcatccccca tcagatttcc agcaaagttc atctgcctgt gaaagttatc
     9181 tttaaagaca ttaaacaatt ttgtcaatcc gttataattt ctgatataag atttatcttt
     9241 ctttggaatt gagtcgagtg tctgctgaat tgcagaattc acaaacccgg gatattcatc
     9301 tgatgtctcc ataaaaagaa ttgtaactcc tgataaattt gcttcaccaa atggagtgat
     9361 agtaaactta ttaagggtga tataattgta tgtgcaaaga gaaattatga ttacagaaaa
     9421 agggattatt aataaaacat attttataag actatatctg tttatgataa aatacaaaat
     9481 aatcaatacc atgatcccaa caaagaataa accagccggt ctgattagga tcaaaattgc
     9541 aacggaaatt gagaataaaa tccaatactt atttttattt tcttttatag aaatcatcat
     9601 caaagcagta caaataagca gcatgtttgt aaaaattcct tcagtaagta atgcagattc
     9661 aaaaagtaaa aaatatgaag atgtcatata aacagtaagg aagcatgcaa atacgactgt
     9721 aaatttttta taaattttat caaataaata tactattatc acacctgtaa aaagagaaaa
     9781 agcactttgc ataactgaaa ctgcaaaaat gcttttcgag aaactccaga tcattgccag
     9841 gaaaagagga tacccgggag tcctgatatc aaacaaaggc ataacagaat tcaaaatcga
     9901 aagggctgct gcaatatatg aggcactgtc atttgataaa atatctacac ggtaatataa
     9961 aagataagga agtcttgtaa taaaaaaaaa tacaagaaaa atacctgtac cttttaaaaa
    10021 tgaatttctg ccattaaata agaaatttct tactgaattc attttaaacg gtttttaaca
    10081 tcgtctgata ccatcaaatg ttttaactga acagagatga gacccattcc aaacataaga
    10141 atagaaagga gtatacttcc aagaccaaga taaaaagtaa ttggcatccg gaatattatt
    10201 gaaaaaataa aattaactcc tgcaaatgca agtaaagcca gacaaaatac aatgaatatt
    10261 ttaatcggat tataaaaaag aattgcttca gtaataaact gcaaagtacg cattgaatct
    10321 ctgaacaatt ttacttttgt tttaccctct ctttttttat aatcaatcgg aatatatttt
    10381 acatacattg cattcatcat gtatgctaat gtaagcgaag tagtaaaact gaatgtattg
    10441 cataattttg aaaaataagg taatactttt gatttagaga atactctcag tccggaattt
    10501 atatcatcta tctttcttcc cgcagaaaat tcaaccagct ttttcagaat aagtctgagt
    10561 acttttttct taaatgactc atcataattt tcaccctgcc tggcacctac gaccatatca
    10621 aaacctttat tatattcact gactaaaacg ggtatcatct ccagaggata tgtgccgtca
    10681 gcatctgtaa taacaattat gtcgttcgtc gcagcttcaa ttccgtcttt aagagattta
    10741 ccataaccga tattatgagg atgcctgata gtaactgcac cggctttttc actcatctca
    10801 gcagtcctgt cagtagagcc gtcatcgact actataatct caatatccct gagattattt
    10861 aattcggcaa ccttctttat gtcaaagaca gtcattgcaa ttacacttcc ttcattatat
    10921 gcaggtatca ccacagagat cataatctaa attattgttt tttaaaaata ctgatcagag
    10981 aaattccgat cggattactg acaggtaaaa atttctttga gaatggaact atgtatttat
    11041 caaacattcc aatctcacct tcggtatcgt tgatgtttcc tgactttaaa gctttatata
    11101 ctacccagta tcctacagct ccgattg
//
LOCUS       DAORVQ010000105        11046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3024_length_11046_cov_5.248112, whole genome shotgun sequence.
ACCESSION   DAORVQ010000105 DAORVQ010000000
VERSION     DAORVQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_11046_cov_5.248112"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLX80_09340"
     CDS             <1..574
                     /locus_tag="PLX80_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HQY21031.1"
                     /translation="DHMFGKNREGNFDTLKELSAEFDFTIDKVDEYKPEGHHVSSSEI
                     RHLLEKGEVSKASLLLGREYSLSGTVTEGKKLGRELGYPTANIKPDCGYKLIPAIGVY
                     SVTAVLEGIENFGMMSIGKNPTVSDDDTIKIEVNIFSFDKDIYGKKIEIKFNEYLRDE
                     IKFDTIEELKEQLLKDKINCEEIFAKIKIN"
     gene            594..866
                     /gene="rpsO"
                     /locus_tag="PLX80_09345"
     CDS             594..866
                     /gene="rpsO"
                     /locus_tag="PLX80_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQY21032.1"
                     /translation="MPLSKEKKQELVTKFGNSDKDSGKPEVQVAILTKRINELSAAHF
                     NKHKKDNHSRRGLLKMVGKRRKLLRYLENKDVQRYRTIIKELEIRK"
     gene            879..3017
                     /gene="pnp"
                     /locus_tag="PLX80_09350"
     CDS             879..3017
                     /gene="pnp"
                     /locus_tag="PLX80_09350"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQY21033.1"
                     /translation="MPITKKIEKSIEIGGRKITLESGKLAKQANGAVMISTGDNFVLC
                     TVTASDEAKPGQDFFPLTVDYREKTASAGKIPGGFFKREGRPTEKEILSSRLIDRPIR
                     PMFPEGFFNEVQLLCSVYSSDGENDTDVLAATGASAALLISDIPFDVPISEVRVTRID
                     GEFILNPNHEQIDNGDFDITIAGSVDSIMMVEGEAKEVSESEIIEAIKFGHKYIAELC
                     NFQLAFAAECGKTKRELLAKEEYTEMDAEIKALVTDDIKNVTHTILTKEERQLKNKDV
                     FKKVVDALSEKYPEMEKQFGKIIHDIQYDVMRSMILDENKRLDGRGLADIREITCEVG
                     VLPRTHGSALFTRGQTQSLTTVTLGTKRDEQMIEGLKDLTYKRFMLHYNFPPFSTGEV
                     GGRPGPGRREIGHGNLAERSLKKLLPNDEDFPYSLRVVSDILESNGSSSMATVCAGSL
                     ALMDAGVKLKKPIAGIAMGLIIENEKSAILSDILGDEDHFGDMDFKVAGSADGITAIQ
                     MDIKVRGISFEIMEKALNQAKAGRLHILNVMDQTLSVARDSISNFAPHLYSMMVPRDM
                     IGAVIGPGGKTIRSIIAESGAQIDIDDEGKVTIAAVDHTQAEIARKMIAGLTEVPEIG
                     KIYDGTVKGIKEFGAFVEILPGKEGLLHISEIDNKRVLKVEDVLKFGQKVQVKLLGID
                     DSGKLKLSRKILLPKEPVALKEPVNPNENA"
     gene            3017..3322
                     /locus_tag="PLX80_09355"
     CDS             3017..3322
                     /locus_tag="PLX80_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY21034.1"
                     /translation="MKFEIDKQDDKTIFKLEEKSLDQSLAVQLKTELVFLQKEINNQF
                     IIDLKDVTKCDSSGLSVLLFSERIERDKERKLMLINVNKNVEDLMKIARLDRVFEIK"
     gene            complement(3384..3965)
                     /locus_tag="PLX80_09360"
     CDS             complement(3384..3965)
                     /locus_tag="PLX80_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HQY21035.1"
                     /translation="MSKNRMEAFSDGVIAIIITILVLELKIPHGGTLNDLVPLIPVFL
                     SYVLSFIYLGIYWNNHHHILQTVTKTNGKILWANLHLLFWLSLLPFVTGWMGENHFEP
                     MPVALYGIVLLLAAIAYFILQTQILKFEGKDSLLAKALGKDTKGKMSPLLYAVAIACS
                     FYNVWLAFGIYIFVALMWLIPDKRIEKKLQNEN"
     gene            4097..4441
                     /locus_tag="PLX80_09365"
     CDS             4097..4441
                     /locus_tag="PLX80_09365"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HQY21036.1"
                     /translation="MKNNFKKICKESDIYNRKGRRFELDEENEIAVFKVEGEIYVVDN
                     VCPHNHTPQIFDGYVEEMYVACPLHGFRFNLKTGEQPTKLGCKLRTFEHKTENGFLFV
                     KIPDEKRFNFEF"
     gene            4466..5395
                     /gene="queG"
                     /locus_tag="PLX80_09370"
     CDS             4466..5395
                     /gene="queG"
                     /locus_tag="PLX80_09370"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQY21037.1"
                     /translation="MIESLSNNIKKLCSKSGFLKTGFSKAEILTNESNVLRQWIDKGL
                     NADMMWMNNSFEKRRDPSLILEEVRSVISLAYFYDTPAEHKDEPTIPKISRYAWGKRD
                     YHKILKKKLKNLSIEIEKLESDTKTKSYIDDGPVMDKVWAARSGIGWMGKNTNIINKD
                     FGSYFFIATILINKELIYDKHVEDLCGSCNLCIEACPTGAIYEEYKLDSNLCISYQTI
                     ENRNEIQNDLKLEGWIFGCDVCQEVCPFTRNKFFTEDENFLPAAGHFNKSYDELMRLN
                     ENEFDEQFNGTPVKRTKFKGWQRNLKKAKEEIS"
     gene            5426..5890
                     /locus_tag="PLX80_09375"
     CDS             5426..5890
                     /locus_tag="PLX80_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21038.1"
                     /translation="MAVEYTMEITKEISKTLIEVISPDELEYFDELADTHFDVRKPNN
                     DAQLGFGLVEFGELATPAVMTLVSGIVAYLMTDVVKVAKDQMNEKIKEKLKGFFSKKK
                     KTEEEKSLTLTKEQLQKISELIDNKLKDIDMNEKTALRIRDSLISSFALGEE"
     gene            5892..7535
                     /locus_tag="PLX80_09380"
     CDS             5892..7535
                     /locus_tag="PLX80_09380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY21039.1"
                     /translation="MKHGEKKLTYENVFDYPIETDNIFRLFLLTITGITIIVGNYIPS
                     FLFDTESSPLAIFSLTEFYDVDKVFNTPPEIFFNNIFLFLKPITIQFTYVLLIFGISY
                     YIYKKRPDKIIKEIDLILIEDEKTIGMLETLRIKAGINDQVSYYTNRNIRDFSGQVFG
                     SGKKMYLKVGRGLLKIISLKNYELFESIIIHEFSHIKNKDIPKTYFAESVLKIPFFIT
                     LAVSTFVMFFALIKSIASKAFSSELTFLIVIEKAAAYLNLFIQITGLLIILSLLFRML
                     IRSREYYADLRTASLIDSSVQIKFFDKSGKKIIEQNFIEKLFGYHPSFIHRSDILKDP
                     VKIFKPSDNISFLNNLISYIFITVALVFGVSLLLLIITLAGYSLLYLLKGVLNEIIHL
                     YLVYFEMIIGTLAIAVFMLLCGNILRKTFFSQVDKILITQKFDKSYKKILPELIRLSI
                     FASLGVICGYLLLPLYGFNILNIQNLLSIPFVFVITFTGFIILNTFHYFWMDHKINNE
                     YKPDFSVPVLLRFFEGVTILMIFLSAILSFQFFTTLIFS"
     gene            7616..7981
                     /locus_tag="PLX80_09385"
     CDS             7616..7981
                     /locus_tag="PLX80_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21040.1"
                     /translation="MKKLFTNSMIIFALLLLVVSTESKADGTEKAKINELSDQIVIEI
                     KEVLSSPYLRYESKDLSGEVTVYTAVSENGKIEFTGLKGINENLIMNISSKLNSLNLW
                     TNPDYNKKVFQFNINYQDK"
     gene            8146..8883
                     /locus_tag="PLX80_09390"
     CDS             8146..8883
                     /locus_tag="PLX80_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HQY21041.1"
                     /translation="MKFYLLLFLAIPLFLNAQDSTKKISDWSGFLSGAGLYQSGNTNK
                     FYIQGAGELKRAGKILETILFGSIGYGENKGRKDENEIIAAFTADLFYENKFSPFILQ
                     YLNYSFAKGIDLRSQSGGGIKYVPIKNEKNKTSISAALIYDYENLTSKPGNFDQDKLR
                     FSFRLKTKQDLFKEHLIFTMVAFFQPDISQVSSANFWIDSNLKVPVHKKISFTVNYLY
                     TFQDVVSVGRVRADNKLTFGMRFDFGQ"
     gene            complement(8849..9940)
                     /locus_tag="PLX80_09395"
     CDS             complement(8849..9940)
                     /locus_tag="PLX80_09395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HQY21042.1"
                     /translation="MEPVNISDFEILAKKKLDKNAFDYFYSGANDEITLKENCEAFKR
                     IFSKYRVLVNVSKRDLTTKILGHNISMPVIIAPTAFQKLAHPDGETASATAAGKNGII
                     MILSTLSNTSVEEVVKASSGPVWFQLYVFRDREITRELIKKAEAAGVKAIVLTVDAPL
                     LGKRENDVRNKFNLPPEFEIANLAFVSKSNLNVKNDSGLTGYVSENLDPSLSWKDIDW
                     LRSVTSLPIILKGIACKEDALLALEHNIEGIVVSNHGGRQLDTCRASIDVLPEISNAV
                     SGKTEILLDGGIRRGTDILKALALGASTVLTGRPVLWGLSHNGEDGVTDVLNIFKNEL
                     DLAMALCGCSDIKKITKELIVQNQISFQR"
     gene            complement(10046..>11046)
                     /locus_tag="PLX80_09400"
     CDS             complement(10046..>11046)
                     /locus_tag="PLX80_09400"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468908.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQY21043.1"
                     /translation="PDQCENAISFGAEGIGLCRTEHMFFGGDRIDAVREMILAYSTEG
                     RKKALLKILPFQRKDFTEIFRVMDGLPVTIRTLDPPLHEFLPHTEIEITKLAELIGTR
                     VDEIKYKLNALREFNPMLGHRGCRLGISFPEITEMQARAIFEAAAVVLKEGKNVYPEI
                     MIPLIGHEKEFIEQEKIIRDTAKQVMKEKKIKFKYKVGTMIEIPRAALVAGKIAEHAE
                     FFSFGTNDLTQMTFGISRDDAGSFLPTYRDKEILLRDPFESIDTEGVGMLMEFAVRKG
                     RETQPNIKLGICGEHGGEPDSIKFCNDLGLEYVSCSPFRVPIARLAAARAELGAKK"
BASE COUNT         3828 a         1707 c         2025 g         3486 t
ORIGIN      
        1 tgatcatatg tttggtaaaa acagggaagg aaattttgat acacttaaag aactgtctgc
       61 cgaatttgat tttacgatcg acaaagtgga tgagtataaa cctgaaggtc atcatgtcag
      121 cagttcggag atcagacatc ttttagaaaa aggtgaagtc tcaaaagctt ctttgctcct
      181 tggcagggaa tatagtctta gtgggacagt tacagaagga aaaaaacttg gcagagaatt
      241 aggttatcca acagctaata taaaacctga ctgcggatac aaattaatac cggcgatcgg
      301 agtgtattca gtaacagcag tgcttgaagg aattgaaaat ttcgggatga tgagtattgg
      361 aaagaatccg actgtatctg atgatgatac aataaagatc gaagttaata ttttcagttt
      421 tgacaaagat atttatggaa agaaaattga aataaagttt aatgaatatc tgcgtgatga
      481 aataaaattt gatacaattg aagaattaaa agaacagctt ttaaaagata aaataaactg
      541 tgaagaaatt ttcgcaaaaa taaaaattaa ttaaataata taaataaaca gaaatgccat
      601 tatcaaaaga aaagaaacag gaattagtaa caaagttcgg aaattccgat aaggattccg
      661 gaaagcctga agtacaggtt gcaattctca caaagaggat caacgaactt tcagcagcac
      721 attttaacaa acataaaaaa gacaatcatt caagaagagg attacttaag atggttggaa
      781 aaagaagaaa gcttttaaga tacctggaaa ataaagatgt ccagagatac agaactatca
      841 taaaagaatt agagatcaga aaataataaa aagattaaat gccaattaca aagaaaattg
      901 aaaagagtat tgaaataggc ggaagaaaaa taaccttaga gtcaggcaaa ctagccaagc
      961 aggcaaacgg cgcagtcatg atttcgacag gtgataattt tgttctatgt acagtaacag
     1021 cttctgatga agcaaagccg ggtcaggatt ttttcccgtt aacagttgat tacagagaaa
     1081 agactgcatc cgctggaaag attccgggtg gattttttaa aagggaagga agaccgactg
     1141 aaaaagaaat actttcatcc agacttatag acagacctat cagacccatg tttcctgaag
     1201 gtttttttaa tgaagtacag cttctttgtt cggtatattc ttctgacgga gaaaatgata
     1261 cagacgtatt agctgcaaca ggagcatcag cagcattgtt gatctctgac attccatttg
     1321 atgtgccgat ctcagaagtt agagttacaa ggatcgacgg tgaatttatt ttgaatccaa
     1381 atcatgagca gattgataac ggtgattttg atattacgat agcaggttca gtggattcta
     1441 ttatgatggt ggaaggtgaa gctaaagaag tttctgaatc tgaaattatt gaagcaataa
     1501 aatttggaca taaatatatc gctgaacttt gcaactttca gcttgctttt gctgctgaat
     1561 gcggtaaaac aaaaagagag ctgttggcta aggaagaata cactgagatg gatgctgaaa
     1621 taaaagcatt agttacggat gatataaaaa atgtgactca cacaatcttg acaaaagagg
     1681 aaagacagct aaagaataaa gatgtcttta aaaaagtagt agatgctttg tcagagaaat
     1741 atcctgaaat ggagaagcaa tttggaaaaa taatacatga tatccagtat gatgtaatga
     1801 gatctatgat acttgatgaa aataaaagac ttgacggaag aggacttgca gacatcaggg
     1861 aaattacctg tgaagtaggt gtactgccca gaactcacgg ttccgcgctt ttcacaagag
     1921 gtcagacaca aagtcttaca actgtaactt tgggaactaa gagagatgag cagatgatcg
     1981 aaggattaaa agatcttaca tataaaagat ttatgcttca ttataatttt cctccgttca
     2041 gtaccggtga agtaggcgga agacccggac cgggaagaag agagatcggt catggaaatc
     2101 ttgcagagag atcactcaaa aaattattac caaacgacga agattttcct tattcattaa
     2161 gagttgtatc cgatattctt gaatctaacg gttcttcttc aatggcaact gtctgtgcag
     2221 gttcacttgc attaatggat gccggcgtta aattaaaaaa acctatagca ggaattgcaa
     2281 tgggtcttat aattgaaaat gaaaaatctg caattctttc agatattctc ggagatgaag
     2341 atcattttgg agatatggat tttaaagtgg caggttcagc tgacggcata acagcaattc
     2401 agatggatat aaaagtaaga ggaatttctt ttgaaataat ggagaaagca ttgaatcagg
     2461 ctaaagccgg cagacttcat attctgaatg taatggatca gacgttatca gttgcaagag
     2521 attctatttc aaattttgct ccgcatcttt attcaatgat ggtaccgaga gatatgatag
     2581 gtgcagtaat tggacctggt ggaaaaacta tcagaagtat aattgcagaa tccggtgctc
     2641 agatagatat tgatgatgaa ggtaaagtta ctattgccgc agtcgatcat actcaggcgg
     2701 agatcgcaag gaagatgatc gccggtctga ctgaagttcc tgagatagga aagatctatg
     2761 atggcacagt aaaaggtatc aaggaatttg gagcatttgt tgaaatactt ccgggaaagg
     2821 aaggtctgct tcacatttca gagatcgata ataagagagt attaaaagtc gaagatgtgc
     2881 tgaagttcgg tcaaaaagtg caggtgaaat tacttggtat tgatgattcc ggaaaattaa
     2941 aattaagcag aaagatctta ttaccaaaag aacctgttgc actaaaagaa cccgttaacc
     3001 ctaatgaaaa cgcataatga aatttgaaat cgataaacag gacgacaaga ctattttcaa
     3061 acttgaagaa aagtctctag accaaagtct tgctgtacag ttgaaaactg aactggtttt
     3121 tttacaaaag gagatcaata atcagtttat tattgacctt aaagatgtga caaaatgcga
     3181 cagttcagga ctttcagttc ttctcttttc agaaagaatt gaaagagata aagaaagaaa
     3241 actgatgctg ataaatgtaa acaagaatgt ggaagatctt atgaagattg cacggcttga
     3301 cagggtattt gaaataaaat aaaatttctt acatatatct taaaaagctt ctcaacggga
     3361 tttccgctga gaagtttttt attttaattt tcattctgaa gttttttctc aattctttta
     3421 tccggaataa gccacatcaa tgctacaaat atatagatcc caaatgctaa ccatacatta
     3481 taaaaagaac aggcgatagc aactgcatat aatagaggtg acatttttcc ctttgtatct
     3541 ttacctaatg ctttagcaag gagactgtct ttaccttcaa attttaggat ctgagtttga
     3601 agaatgaagt aagctattgc agccaaaaga agtacaatac catataatgc taccggcatt
     3661 ggttcgaaat gattctcacc catccagccg gttacaaaag gtaaaagaga aagccagaaa
     3721 agtaaatgca gatttgccca taggatcttt ccgtttgttt ttgtaacagt ctgcaaaata
     3781 tgatgatggt tattccagta tattccaaga taaataaaac taagtacata acttaagaaa
     3841 acaggaataa gcggaacaag atcattcaaa gtgccaccgt gcggaatttt caattcaaga
     3901 acaagtatgg taattattat tgctattact ccatcactga atgcctccat tctgttttta
     3961 ctcattatat tttttatata aattttttaa ttcagataat ttaattatta caagtttgtt
     4021 aatccattta aaaacagtat ttataaaacc aaacttttaa actttattta aattttaatc
     4081 tgcttattat tgacaaatga aaaataattt caaaaagatc tgtaaggagt ccgatattta
     4141 taacagaaaa ggcagacggt ttgaactgga tgaagaaaat gagatcgcag tttttaaagt
     4201 cgaaggtgag atctatgtag tggataatgt gtgtccccac aatcacactc ctcagatatt
     4261 tgacgggtat gtcgaagaaa tgtatgtagc atgcccattg cacggatttc ggttcaatct
     4321 gaagacaggg gagcagccta ctaaacttgg atgcaaactc agaacttttg agcataaaac
     4381 agaaaacgga tttttatttg tgaaaatacc tgatgagaaa agatttaact tcgagttttg
     4441 aataaagtaa actgaaaata tttaaatgat agaaagtctt tcgaataata taaaaaaatt
     4501 gtgcagcaag tcgggatttt taaagacagg attttcgaaa gctgaaattt tgacaaatga
     4561 atccaatgtt ctcagacaat ggatagataa aggtttgaat gctgatatga tgtggatgaa
     4621 taacagtttc gaaaaaagaa gagatccatc tttgattctt gaagaagtca gatctgttat
     4681 ctcacttgcc tatttttatg atacacctgc tgagcataaa gatgaaccta ccattccaaa
     4741 aatttcaaga tatgcctggg gaaaaaggga ctatcataaa atattgaaaa agaaattaaa
     4801 gaacctgagt attgaaattg aaaaacttga gtctgacaca aaaacgaaat catatattga
     4861 tgacggacct gtcatggata aggtctgggc tgcgagatca ggtatcgggt ggatgggaaa
     4921 aaatacaaat ataataaata aagattttgg aagttatttt tttatagcaa ccattttaat
     4981 aaataaagaa ctgatctatg ataagcatgt cgaagatctt tgcgggtcat gtaatctgtg
     5041 catcgaagcc tgtccgaccg gtgcgatcta tgaagaatac aaactcgatt caaatttatg
     5101 tatctcttat cagacaattg aaaacagaaa tgaaattcaa aatgatctaa aacttgaagg
     5161 atggatattc ggatgcgatg tgtgtcagga agtatgtccg tttacgcgga ataaattttt
     5221 tactgaggat gaaaatttct tacccgctgc aggacatttt aataaaagct atgatgagtt
     5281 aatgaggttg aatgaaaatg agtttgatga acaattcaac ggtacaccgg taaagaggac
     5341 aaaatttaaa ggctggcaga gaaatcttaa aaaagctaaa gaagaaattt cataaactac
     5401 atattatttt aattaaatta tttttatggc agtagaatac acaatggaaa tcactaaaga
     5461 aatttccaaa actttaatcg aagttatttc tcctgatgag ctggaatatt ttgatgagct
     5521 tgcggataca catttcgatg ttcgtaaacc gaacaatgat gcgcagctcg gatttgggtt
     5581 ggtggaattc ggcgagctgg caactcctgc agtgatgacc ttggtatctg gcattgttgc
     5641 ttatctgatg acagatgttg tgaaagttgc aaaagatcaa atgaatgaaa agataaagga
     5701 aaagctgaaa ggcttttttt caaaaaagaa aaaaactgaa gaagaaaaat cactgacact
     5761 tacaaaagag cagctgcaaa agatctctga actaattgac aataaactga aagatattga
     5821 tatgaatgaa aagactgcac ttcgaatccg tgattcattg atatcatcat ttgcattggg
     5881 agaggaatga gttgaaacat ggtgaaaaaa aattaactta tgaaaatgtt tttgattacc
     5941 cgatcgagac ggataatata ttccgtcttt ttttactgac aataacaggt attacaatca
     6001 tagtcggaaa ttatatccct tcatttttat ttgatacaga atcttcacct ctcgcaatat
     6061 tttcattgac agaattttat gatgtcgata aagttttcaa tacgccaccc gaaatatttt
     6121 tcaataatat atttttattc ctgaaaccaa tcacaatcca gtttacttat gtgcttctga
     6181 tctttggaat ttcttattat atttataaga aaaggcctga taaaattatc aaggaaattg
     6241 atctgatatt gatcgaagat gagaaaacta ttggtatgct ggaaacattg aggataaagg
     6301 cagggataaa tgatcaggta agctattata ctaataggaa tatcagagac ttcagcggac
     6361 aggtttttgg ttcaggaaaa aaaatgtatc taaaagtagg aagaggtctt ttgaaaataa
     6421 tttcattaaa aaattatgaa ctgtttgaaa gtattataat tcatgaattt tcccatatca
     6481 aaaataaaga cataccaaaa acttattttg cggaatcggt attaaagatt ccttttttta
     6541 ttacccttgc agtttccaca ttcgttatgt tttttgcttt gatcaagagt atcgcttcga
     6601 aagcttttag cagtgaactt acatttctaa ttgtaatcga aaaagcggct gcctatttga
     6661 atttatttat tcaaataaca gggctcctga taatactttc ccttcttttc agaatgctta
     6721 tcagaagccg tgaatattat gctgatctga gaacagcttc attaattgat tcttctgttc
     6781 agataaaatt ttttgataaa tccggtaaaa agatcattga acaaaatttt attgaaaaat
     6841 tattcggata ccatccttca tttattcaca gatcagatat acttaaagat ccagttaaga
     6901 tatttaaacc ttccgataat atttcttttc tgaataatct gatttcatat atttttatta
     6961 cagttgcatt ggtatttgga gtatcacttt tattgttgat aataacttta gcaggttatt
     7021 ctttgcttta tcttctgaag ggagttttaa acgagatcat tcatttgtat ttagtttatt
     7081 ttgaaatgat aataggtaca ctagctattg ctgtgtttat gcttctttgc gggaatatat
     7141 taaggaagac ttttttttcg caagttgata aaatacttat cacacaaaag tttgataaaa
     7201 gttataagaa aatattacct gagctgatca ggctttcgat atttgcttca cttggtgtca
     7261 tctgtggtta tcttctgttg cctttatacg gattcaatat tctgaatata caaaatcttt
     7321 tatcaatacc atttgtgttt gtaataacat tcacaggatt tataatacta aatacttttc
     7381 attatttctg gatggatcat aaaataaata atgaatataa acctgatttt tcagttcctg
     7441 tattactcag atttttcgaa ggagtaacca ttttaatgat ctttctttcc gcaattctgt
     7501 cattccagtt ctttacaaca ctaatattca gttagatctg tctgtttgaa acttatcact
     7561 tttaatctcg aataaaccta tgtcctacaa aaaaattaat ataaggagta aagaaatgaa
     7621 aaagttattt acaaattcga tgattatttt tgctttattg cttttagtgg tctcgactga
     7681 atcaaaagct gatggaactg aaaaagctaa aattaatgaa ctgagtgatc agatagtaat
     7741 tgaaataaag gaagtattaa gctcaccata tcttagatat gagagcaaag atctctccgg
     7801 tgaagtaaca gtttatactg ctgtttctga aaatggtaaa atagaattca caggattgaa
     7861 aggaattaat gaaaatctga taatgaatat aagctcgaag ctgaattcgt taaacctatg
     7921 gactaatcca gattacaata aaaaagtatt tcagttcaac attaattacc aggataaata
     7981 attaaaattg ttacaaccct caaaatccct gtaaggtaaa agctttacag ggattttatt
     8041 tttgtataaa taattttttt ttaaaatgct ttcatttatt ataaaaatga gtatttgtat
     8101 tttaaatttc ttttaaaaat tagttataaa acttataaag gttaaatgaa attttattta
     8161 ttactttttt tagcaattcc gttattttta aatgctcagg attctacaaa gaaaatatct
     8221 gattggtctg gatttctatc aggtgccgga ctttatcaaa gtggtaatac aaataaattt
     8281 tatattcagg gtgcagggga attgaagcgg gcaggtaaaa ttcttgagac tattctattt
     8341 ggatccattg gttatggaga gaataaaggt cgaaaagatg agaatgaaat aattgcagca
     8401 ttcacggctg atctgtttta tgaaaataaa ttttctccgt ttattctaca gtatctgaat
     8461 tacagttttg caaagggtat tgatctgcgt tcacagtcgg gtggtggtat taaatatgtt
     8521 cctataaaaa atgaaaagaa taaaacatcc atatcagcag ctttgatcta tgattatgaa
     8581 aatcttacct ccaagccggg aaattttgat caggataaat taagattttc attcagacta
     8641 aaaacaaaac aggatctttt taaagagcat ttgatattta caatggttgc attttttcag
     8701 ccggatatat cacaggtttc aagtgcaaat ttctggatcg attcaaatct gaaggttccg
     8761 gtacataaaa aaatttcttt cacagtaaat tatttatata cttttcagga tgtcgtctcc
     8821 gttggaagag taagagctga taataaactt acctttggaa tgagatttga ttttggacaa
     8881 taagttcttt tgtgattttt tttatatctg agcagccaca caaagccatt gcaagatcaa
     8941 gttcattttt gaaaatattc aatacatcag taactccgtc ttcaccatta tgagataatc
     9001 cccacagcac aggtctgccg gtcaggacag tgcttgctcc gagagcaagt gctttcagaa
     9061 tatctgtacc tcttctaatt ccgccgtcca ataatatttc tgttttacca cttacggcat
     9121 tggatatttc gggaagtacg tcaattgatg ctctgcaagt atcaagctgt ctgcctccgt
     9181 gatttgaaac tacaatacct tcaatattat gttctaaagc aagcagtgca tcttctttgc
     9241 atgcaatacc ttttaagata attggtagag atgtaacaga tctcagccag tcgatgtcct
     9301 tccagcttag agaaggatca agattttcag atacataacc tgtcagtcca gaatcatttt
     9361 ttacattcag gtttgacttt gatacgaaag ccagatttgc aatttcaaat tccggaggaa
     9421 ggttgaattt atttctaaca tcattttctc tcttacccag caaaggagca tcaactgtca
     9481 gcactattgc tttgactcca gcggcttcag ctttcttaat aagttctcta gtaatttctc
     9541 tgtctctgaa aacatataat tgaaaccaaa ccggtcctga tgaagctttc acgacttctt
     9601 ccactgatgt atttgataaa gtgcttaaga tcattatgat accgtttttc cctgcagctg
     9661 ttgcagaagc agtttcacca tcaggatgag ctaatttttg aaatgcagtc ggtgcaatta
     9721 ttactggcat ggatatatta tgaccaagaa tttttgttgt cagatctctt ttactaacat
     9781 tcacaagaac tctgtatttc gaaaaaattc ttttgaatgc ttcacaattt tcttttaaag
     9841 tgatttcatc atttgctccg ctgtaaaaat aatcgaaagc attcttgtct aattttttct
     9901 ttgcaagaat ttcaaaatca gaaatattaa cgggttccat aaaattaatt tttaaagaaa
     9961 atctcagggt ataaataatt tcaaaaaatt aaaaatacct tgagataaca aaagtttcaa
    10021 gctaagaata atttcaaaag agatattact tttttgctcc aagctcagct ctggctgcag
    10081 caagtcttgc tattggcact ctgaatggag agcaactgac atattcgaga cccaggtcat
    10141 tacagaattt tatagagtcg ggctcaccgc cgtgttcacc gcaaattcct aattttatat
    10201 ttggctgagt ctctctgcct tttcttactg caaattccat taacataccg acaccttcag
    10261 tatctataga ttcaaaagga tcacgtaata aaatttcttt atctctgtaa gtaggaagga
    10321 atgaacctgc gtcatctctt gaaattccga aagtcatttg agtcagatcg tttgtaccaa
    10381 atgagaaaaa ctctgcatgt tcagcgatct tacctgctac gagtgcagct cttgggatct
    10441 cgatcattgt cccgacttta tatttaaatt taattttctt ttccttcata acctgttttg
    10501 ctgtatctcg gattattttt tcctgctcaa tgaattcttt ttcatgtcct atgagaggta
    10561 tcattatttc cggatataca tttttacctt cttttagaac tacagcagca gcttcaaata
    10621 ttgctcttgc ctgcatttca gtgatctcgg gaaatgatat gcccagacgg catcctctgt
    10681 gcccgagcat tggattaaat tctctgagag cattaagttt gtattttatt tcatcaactc
    10741 tggtaccgat tagttctgca agtttagtga tctcaatttc agtgtgagga aggaattcat
    10801 gcagtggcgg atcaagtgtt ctaatagtca ccggtagtcc gtccataacc cgaaatattt
    10861 cggtaaaatc ttttctctga aacggaagaa ttttcaaaag agcttttttc ctgccttcag
    10921 tactataagc aagaatcatt tctctgacag catctattct gtctccgcca aagaacatgt
    10981 gctcggttct gcaaagtcca ataccttctg caccaaatga aatcgcattt tcacactgat
    11041 ccggtt
//
LOCUS       DAORVQ010000106        11001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3042_length_11001_cov_4.483556, whole genome shotgun sequence.
ACCESSION   DAORVQ010000106 DAORVQ010000000
VERSION     DAORVQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_11001_cov_4.483556"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..8085)
                     /locus_tag="PLX80_09405"
     CDS             complement(<1..8085)
                     /locus_tag="PLX80_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY21044.1"
                     /translation="MGKAQNLVDLLIYRAQKQPDKTAFVFLEDGETEAGKFSYKALDI
                     SAKRIAESLQIKNLNGERALLIYESGLEYLDSFFGCLYAGVISVPLHPPGKNKSLSRI
                     AAIAKDSGAKVILSTSEIYEELKDEFEKDNFLKDLEWILTETISDINSEKWINPGIKP
                     ETLAYLQYTSGSTGIPKGVMVNHKNLLANLEIIDKSHPHNDKSVMVTWLPIHHDMGLI
                     YGILLPFYSGYPCYFMTPQAFVQKPHRWLSAISKFKGTHNAAPNFAFELCVNKISDDQ
                     KKSLDLSTWTTAMNAAEPVRAETIERFSEYFSECGFEKRFFSPGYGLAEGTLILSTTF
                     TKDEPVLKRFRDADIEKKNIAVASNSEDKISKVHAGHSHSIEDTRIAIVDPVKLIECK
                     EGEVGEVWASGTSIAQGYWQREDATEETFNAHIKDTNEGPFLRTGDLGFIFENELYIT
                     GRYKDLIIIRGQNHYPQDIEYTVEASHPALRLGCVAAFSIDYEGEEHLGIVQEIQKNF
                     VKDFDTDEIIKAIRKSVSEEHDLQVQSITLIKPGTVPKTSSGKIQRKACLQGVLDNEL
                     EVLAEWKQGELKDHTPKEISDKEKIKFKATSFNLKNWLVKRLSDLMQISIESIDVSES
                     FAAYGMDSLKAVQLSGDLETLLERHLPDTLVYDYPNVNALSEFLSTENVQPADEINEN
                     IISKEISKNENEPIAIVGIGLKFPDADGPDEFWKLLKEGRDAITKIPEGRWKESSMKP
                     STVRFGGFVKDTDKFDPVFFGISPREALQIDPQQRFTLEVAWNAMEDAGIDPSTLAGK
                     DVGVFMGICTYDYARFSAGRKEFFDVYTGTGTSLSIAANRISYLFDFRGPSIAIDTAC
                     SSSLVALHTACNSIRNGESTIALAGGVNLLLAPDWNVVFTEADMLATDGKCKTFDSAA
                     DGYVRSEGCGVVVLKKLSDAIKDGDRIYSVIKGSAINQDGKSNGLTAPNGPSQENVIR
                     KALNNAGIKSSEVSYIETHGTGTPLGDPIEVNSIVKVISKDSSNDYPCYIGSVKTNIG
                     HLEAAAGIAGVIKTSLALYNKKIPKHLNFISLNPEIHIDGTSIKIADTEINWSDNKRY
                     AGVSSFGFGGTNAHIILENAPEELKASNSVSKKMPFNILTLSAKSDKALKQLAGNYIE
                     LLSENNLNTEDICFTANTGRASFNHRLALVCSDNVSLSKQLNNFLFDKNSFGLLKGTT
                     KINHIPKIAFLFPGQGAQFIGMGKEMYDTHPLFRSIINRCDEILRNYLDKPLLEVLFY
                     EKDEKLNPINETTYTQPALFSIEYALGKLWMSWGINPDVMMGHSAGECVAACLAGVYS
                     LEDGLKLVTERGRLMQTMTEKGEMYTIFEDEKVTKEFLKGYEDKVSIASINGPKKTVI
                     SGSKDSLDKIMANIESKNIEYKKIIVSIASHSPLMNSMIDEFRKVCETINYSSAEIPV
                     VSNMTGEIVTDKISNAEYWCKQILSPVRFSESIKECVNNGVEIFVDLGPKPSSINMAQ
                     ESVLDPKIKWLPSFKYNFTIWETLLSSLGSMYVSGIFPDWKEFSKDFSHAKVSLPNYP
                     FQRERYWIGDGMGEMTGETDLSVSVGTAESQSDGGSFAGVRLPSASDTESIFNLRISG
                     NSPEYLKDHVVFGKIIFPAAGFIEMALAVSRSITKNKNINITGILFHQALTLKENEYS
                     DIQIILTKTVPEIYEFKIYSADYSNNNNRNNFSWTLHSSGKINFSKVEAKENKIEVDK
                     IVFNNSHEIKNLYECIKESGVEYLYAFQAIKELNVNGDEAFSKIEMNNIGTIENLAHP
                     VLIDNAFQTVLSLLDYKNPEKAYIPVGVNEIYLYKNLTSVIYCHAGLVKSVSPAVMNS
                     ADIKIYSDNGELVCNIKGLQLKEVSKKEFLAINDDLNGWFYNIDWVKIKKDLIFPKPL
                     ELLQDLIDNSDDQNNFDTSANSVSKYKDFIKDLELLTVHYIISAIKESGFKTSIGNSL
                     KVNEFFESFGFSEKYKKLANRLIEILSDNNFLKIDKDEIEFNVGHDIDPSDILPDVLC
                     KKYPDFLAEIKLTELCGKQLFNILTGKKDPLEILFPEGDMTLLTKLYQDSPAFVHMNN
                     SIRKITGNIQSSFTEGKKLNILEIGAGTGSTTSYITSLFSNENINYTFSDISPAFFIK
                     AKERFKEFNNINFKVLDIEKDPIKQGFVLNEFDIIIASNVIHATKDLINSMNNIGKLL
                     SENGTLILNEVTEKQAWLDITFGLTDGWWNFSDTDLRNEHPLLNNQSWNNLFLQSGFE
                     NAEIYSPELNNKIYTGQSLIIASVNTVENEKIYEVSAENEILVFSIGSVSKNLIEAFK
                     KNAEDIIFINEDSSFEKKSDKEFSVDFNNHIQLKDLFKVFNESNNKKNIIVIIPDSGI
                     VNDDGINKITLKGSSFILNLLKVLSETETSNNYKLTIVTQNAVHVCESETVSGIQNSP
                     IWGIKKVISLEHPELNTKIIDIDFSTKSELIVNEVLSEIEDDMISFRNNCKFIPFLKR
                     IKPDYSKIELDSEGIYMITGGLSGLGLITAKWLSEKGAKNLALLSRRDNTSEADKILN
                     EIRSSGTDVKIFKCDITLKEDLQRIMTEINLSGKKLRGVIHSAGLLEDGILINQTVEK
                     FDKVLSPKIKGAWNLHELTKDLEIDFFIMYSSIASILGSAGQANHSAANSFLDSFAYY
                     RKAKGLPAMSINWGVWSEIGSAVKKGADKNEKIPGLGLIDP"
     gene            8333..8791
                     /locus_tag="PLX80_09410"
     CDS             8333..8791
                     /locus_tag="PLX80_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQY21045.1"
                     /translation="MTSKAKSIAEYLDALPSERKTAVSKLQKCIKKKLPKGFKEEISY
                     GMIGYVVPQSLYPDGYHCNPELPLPFMSIASQKNFIALYHMGLYADKKLLDWFTNEYS
                     KTVKSKLDMGKSCIRFKKPEEIPYKLIGELASKVSPQDWISTYENILKKK"
     gene            8825..9295
                     /locus_tag="PLX80_09415"
     CDS             8825..9295
                     /locus_tag="PLX80_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21046.1"
                     /translation="MVFEKIYNFERNKYNLNGFHKNKILYLFIDFIKECEEDFFDEFD
                     PYFANAFYANSSVLLLLKSCFCTEDNEIFGMESKDGEIDIEMNMEMEEFSENKTIYSL
                     SSSIKGKEDEHLHMIIDNIYSDSQFALKYVPEDEEDDENMEFITDPECKLSLLV"
     gene            complement(9323..10591)
                     /locus_tag="PLX80_09420"
     CDS             complement(9323..10591)
                     /locus_tag="PLX80_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY21047.1"
                     /translation="MINTTLENELTIDENFNLFKALSLKENIQDPYPVYQKLRDYEPV
                     FYMKSPTGFLSENIWILTRYDHISSVLINKKFGRGNKFGKGKITANYARLNSLTQMRQ
                     HWVNFLDPPEHTRIRGFINKAFSPKLVNVLKPNIDSIVNYLLDKFQGDTIEFISEFSY
                     PLAAITIAEFYGIPKEDRDILDKWAMQIVRTLDVVTNQFTQEDLKNIYSSADEIKEYF
                     RKVVDQKEKNPKDDLISRLITIEDNGDKLTKEEIIASLVLMIMDAHEAPKNQISNGLY
                     ALLKNPNQYEKLVKDPSLADGAADELLRYDSPAQFTGRRTHEDETVGGKEIKKGLQII
                     CMLGAGNRDPLKFPEPDRLDIERKKVFPLSFGGGIHFCAGAGFAKLEAVSAFENIAKR
                     FPRLSLMDKEYNFHESLHSRGLDRLELKIK"
BASE COUNT         3501 a         1961 c         1596 g         3943 t
ORIGIN      
        1 aggatctatc agaccaagac ccggtatttt ttcattcttg tctgcaccct ttttcacagc
       61 agaaccaatt tctgaccaga caccccagtt aatactcatt gcaggaagtc cttttgcttt
      121 tctgtaatat gcaaatgaat ctaagaatga atttgctgct gagtgatttg cctgacctgc
      181 agatccaagt attgatgcaa ttgatgaata cattataaag aaatcaattt ctaaatcttt
      241 tgtaagttca tgcagattcc aggcaccttt aatttttgga gacaatactt tatcgaattt
      301 ttcaactgtc tgatttatca aaattccatc ttctaaaagt cctgctgaat gtataacccc
      361 tcttaatttt ttccccgata aatttatttc agtcattatc ctttgcagat cctctttcaa
      421 tgtgatgtca catttgaata tttttacatc agtaccggat gatctaatct catttaatat
      481 tttatcagct tcacttgtat tatctcttct gctcagtaaa gctaaattct ttgcaccttt
      541 ttcagaaagc cattttgcag taattaatcc aagtccgctc agaccaccgg tgatcatata
      601 aatgccttcc gaatctaatt ctatttttga atagtcaggt ttaattcttt ttagaaatgg
      661 tataaactta caattatttc taaaagaaat catatcatct tcaatttcag acaatacttc
      721 atttacaatt aattctgact tagtgctaaa atcaatatca atgatctttg tattaagttc
      781 agggtgctca agagaaataa cttttttaat tccccatata ggtgaatttt gaattccgct
      841 gactgtttca gactcacaaa catgaacagc attctgtgtt acaattgtta atttataatt
      901 atttgaagtt tctgtttcag ataaaacttt taatagattc agaataaatg atgatccttt
      961 taatgtaatt ttatttatac catcgtcatt aacaattcct gaatccggaa ttataactat
     1021 aatgtttttc ttattattac tttcattaaa aactttaaat agatctttta actgaatatg
     1081 attattgaaa tcaacggaga attctttatc tgattttttc tcaaacgaac tatcctcatt
     1141 aataaaaata atatcttcag catttttttt aaaagcttca ataagatttt ttgaaacgga
     1201 tccaattgaa aatacaagta tttcattctc tgctgaaact tcatatattt tttcattttc
     1261 aacagtattt accgaagcaa taattaagga ctgaccggta tatattttat tatttaattc
     1321 tggagaatat atttctgcat tttcaaagcc tgattgtaag aataaattat tccaactttg
     1381 attattcaat aaagggtgtt catttcgaag atcagtatca gaaaaattcc accaaccatc
     1441 tgtcagacca aatgtaatat caagccatgc ctgtttttca gttacttcat ttaaaattaa
     1501 agtgccgttt tctgacaata attttccaat attattcatt gaatttatca gatctttagt
     1561 agcatggata acattggaag caattattat atcaaattca ttcaaaacaa atccctgttt
     1621 gatgggatct ttttcaatat ctaaaacttt aaaattaata ttattaaact ctttaaatct
     1681 ttcctttgct ttgataaaaa atgcaggtga tatatctgaa aaagtatagt taatgttttc
     1741 attactaaag agggaagtaa tataagatgt tgtactacca gtgccagcac cgatctcaag
     1801 tatatttaat tttttacctt ctgtaaaact actttgaata tttccagtaa tttttctaat
     1861 tgaattattc atatgaacaa aagcaggtga atcctgataa agttttgtaa gtaacgtcat
     1921 atctccttca ggaaacaaaa tctcaagcgg atcttttttt cctgttaaaa tattgaaaag
     1981 ttgtttgccg caaagttcgg ttagttttat ctctgccaga aaatccggat attttttaca
     2041 aagtacatca ggcaatatat cggatggatc aatgtcatga cctacattaa attcaatttc
     2101 atctttatcg atctttaaaa aattattatc tgacaatatt tcaatgagtc gatttgctaa
     2161 ctttttgtat ttttcagaaa aaccaaaact ttcaaaaaat tcattaactt ttaaactatt
     2221 tccaatagat gttttgaaac cggattcttt aattgctgaa attatataat gaacagttag
     2281 caattccagg tcttttataa agtctttgta tttagaaaca gaattagctg atgtatcaaa
     2341 attattttga tcatcagagt tatcaattaa atcttgtaat aattctaacg gttttggaaa
     2401 tattaaatct ttcttaattt tcacccaatc gatattataa aaccagccat ttaagtcatc
     2461 attaattgca agaaattctt ttttgcttac ttcttttagt tgcagacctt ttatattaca
     2521 aactagttca ccattgtcag aatagatctt tatatcagca gaattcatta cagcaggtga
     2581 aacacttttt actaatcctg catgacaata tattacagag gtaagatttt tatataaata
     2641 tatctcattt actccaacag gaatgtatgc tttttcagga tttttataat ccaataatga
     2701 aagtacagtt tggaaagcat tatcgattaa aactggatga gctagattct caattgtgcc
     2761 aatattattc atttctatct tactaaaagc ctcatcacca tttacattta actctttaat
     2821 agcctggaat gcatatagat attccactcc ggattctttt atacattcat acaaattttt
     2881 tatttcatgg ctattattaa aaacgatctt atcaacttct attttattct ctttagcttc
     2941 taccttacta aaattaattt ttccggaact gtgcaaagtc caggaaaagt tgtttctatt
     3001 attattatta gaatagtccg cactataaat tttaaattca taaatttccg gaactgtctt
     3061 tgttaaaatg atttgaatat cagaatattc attttccttt aatgtcagtg cctgatgaaa
     3121 taaaattcct gtaatgttta tatttttatt ctttgtaata cttcgggaaa cagctaaagc
     3181 catttctatg aacccggcag caggaaatat tatctttcca aatacaacat gatctttcag
     3241 atattccggt gagtttccgc tgatccgtaa attaaaaata gattctgtat cagaagctga
     3301 aggcagtctc actcctgcaa aagatccgcc gtccgattga ctttcggcag ttccgacaga
     3361 aacggataaa tccgtttctc cggtcatctc tcccattcca tccccaatcc aatatctctc
     3421 cctctgaaat ggataattcg gcaaactcac tttcgcatgt gaaaaatctt ttgaaaattc
     3481 tttccagtca gggaaaattc cgcttacata catacttccc aaactactta acagagtttc
     3541 ccaaattgta aaattatatt tgaaactcgg tagccatttt atcttcggat ccagaacact
     3601 ttcctgagcc atattaattg acgaaggttt aggaccaaga tctacaaata tctccactcc
     3661 attattaaca cattccttta tactctctga aaatctgacc ggtgataata tttgtttgca
     3721 ccagtattca gcatttgaaa ttttatcggt aactatttca cctgtcatat tagataccac
     3781 aggaatttct gcacttgaat aatttattgt ctcacacact tttctgaact catctatcat
     3841 cgaattcata agaggggagt gagatgcgat tgaaacaatt atttttttat attcaatgtt
     3901 cttggattca atattggcca tgatcttatc aagcgaatct ttgcttccgg aaattacagt
     3961 tttcttcggt ccgtttattg acgctatgga aactttatct tcataaccct tcaaaaattc
     4021 tttggtaact ttttcatctt caaagatcgt gtacatctca cctttttctg tcatggtctg
     4081 cataagtcgt cctcgttctg ttacaagttt taatccgtcc tcaagactgt aaactcctgc
     4141 cagacatgca gctacacatt ctccagcgct gtgtcccatc attacatctg gatttattcc
     4201 ccatgacatc cataactttc caagagcata ctcaattgaa aataaagccg gctgcgtata
     4261 agttgtttcg ttgatcgggt ttaatttttc atctttctca taaaataaaa cttctagtaa
     4321 tggtttatct aaataatttc ttaatatttc gtcacacctg tttataatac ttctaaagag
     4381 aggatgtgtg tcatacattt cttttcccat tccgatgaac tgtgctccct gtcccggaaa
     4441 taaaaatgca attttaggaa tatgattgat ctttgttgtt ccttttaata gaccaaaaga
     4501 atttttatca aataaaaaat tatttagctg cttcgataaa cttacattgt cagaacaaac
     4561 taacgctagc ctgtgattaa atgatgctct gcctgtattt gcagtgaagc aaatatcttc
     4621 tgtattcaaa ttattttcag ataacagttc aatataatta cctgcaagct gtttcaatgc
     4681 cttgtcactt tttgcactaa gggtaagaat attgaaaggc attttttttg aaactgaatt
     4741 tgatgctttt aattcttcgg gagcattctc aagaataata tgagcatttg ttccaccgaa
     4801 tccaaacgaa cttacgcctg catatctttt attgtctgac cagttgatct cagtgtcagc
     4861 gatctttatt gaagttccgt ctatatgaat ctcaggattg agtgaaataa aattcagatg
     4921 ctttggaatt tttttattat ataaggctaa acttgttttg atcactcctg caattcccgc
     4981 agcagcttcg agatgaccaa tgttggtttt tactgatccg atgtaacatg gataatcatt
     5041 tgaactgtct ttggaaatta cttttacaat tgaattcact tctataggat caccaagcgg
     5101 tgtacctgta ccatgtgttt caatataact aacttccgaa gattttattc cagcattgtt
     5161 caatgccttt ctgatcacat tttcctgtga tggaccgtta ggtgcagtaa gaccattact
     5221 ctttccatcc tgatttattg ctgaaccttt tattacagaa taaattctgt caccatcctt
     5281 tattgcatct gaaagttttt tcaaaactac aaccccgcat ccttcactcc tgacataccc
     5341 gtcagctgcc gaatcaaatg ttttacattt accgtctgtt gccagcatat cagcttctgt
     5401 aaagactaca ttccagtctg gtgcaagaag taaatttact ccgcctgcaa gagctattgt
     5461 ggattctccg tttcgaatgc tgttgcatgc tgtgtgaagt gctactagag atgatgagca
     5521 tgctgtatct attgctatac tcggtcctct gaaatcaaat aaatatgaga ttctgtttgc
     5581 tgctatgctg agtgaagttc ccgttcctgt gtacacatca aaaaattctt ttctccctgc
     5641 agaaaatctt gcataatcat aagtgcatat tcccataaag accccaacat cttttcctgc
     5701 aagtgtagat ggatcgatgc cagcatcttc cattgcattc caggcaacct caagtgtaaa
     5761 cctttgctgt gggtcgatct gtaatgcctc tcttggagat attccaaaaa ataccggatc
     5821 gaatttatca gtgtctttta caaaaccacc aaatctcaca gtcgatggtt tcatcgaact
     5881 ttctttccat ctgccttctg gaatttttgt aattgcatct cttccttctt taagcaattt
     5941 ccagaattca tcaggtccgt cagcatccgg aaattttaat ccaattccaa ctatagctat
     6001 aggttcattt tcattttttg aaatttcttt ggaaattata ttttcattga tctcatctgc
     6061 aggttgtaca ttctctgttg aaagaaattc tgataacgca tttacattcg gataatcata
     6121 aactaatgta tcgggtaaat gcctttccag taaagtctcg agatctcccg aaagctgaac
     6181 cgcctttaaa gaatccattc cgtatgcagc aaatgattct gaaacatcaa ttgattcaat
     6241 actgatctgc ataagatcgg acagtctttt gacgagccag ttttttaaat taaatgaagt
     6301 tgctttgaat tttatttttt ctttatctga aatttcttta ggtgtgtgat ctttaagttc
     6361 accttgcttc cattcggcaa gtacttccag ttcattatct aatactccct gcagacatgc
     6421 cttcctctga atttttccgc tggaagtttt tggtacagtg ccaggtttaa ttaatgtaat
     6481 tgactgcacc tgaagatcat gttcttctga aactgactta cggatcgctt ttataatttc
     6541 atctgtgtca aaatctttca caaaattttt ctgaatttcc tgaactattc caagatgctc
     6601 ttctccttca tagtctattg aaaaagctgc tacacaacct agccgcagtg caggatgact
     6661 ggcttcaact gtatattcaa tatcctgtgg ataatgattt tgtccacgga taattatcag
     6721 atctttatat cttcctgtta tgtataattc attttcaaaa ataaatccaa gatcacctgt
     6781 cctcaaaaaa ggtccttcat ttgtatcttt aatgtgagca ttgaaagttt cttcagtcgc
     6841 atcttcacgt tgccagtagc cctgagcaat tgaagtacca gatgcccata cttctccaac
     6901 ttcaccttct ttgcattcga tcagcttaac tggatcaacg attgctattc ttgtgtcttc
     6961 aattgaatga ctatgacctg catgtacttt ggaaatttta tcttcagaat ttgaagcaac
     7021 tgcaatattc tttttttcaa tatctgcatc tctaaatctt tttaaaacag gttcgtcttt
     7081 tgtaaaagta gtcgaaagaa ttaaagtacc ttcagcaaga ccgtatccag gactaaaaaa
     7141 tcttttttca aatccgcatt ctgaaaaata ttctgaaaat ctttctattg tctctgctcg
     7201 gacaggctca gctgcattca ttgcagttgt ccaggtactg agatctaaag attttttttg
     7261 atcatcagaa attttattaa cacaaagttc aaatgcaaaa tttggtgcag cgttatgagt
     7321 gcctttaaat tttgaaattg ctgacaacca tctgtgtggt ttctgaacaa atgcctgtgg
     7381 agtcataaag taacatggat atccgctgta gaagggaagt aaaattccat atatcaatcc
     7441 catgtcatgg tgaatcggaa gccacgttac catcacactt ttatcattat gtggatgaga
     7501 tttatcgata atttcaagat tagccaaaag gtttttgtga tttaccatca cgccttttgg
     7561 tatgcctgtt gatcctgaag tatattgtaa atatgccaat gtttcgggct taatacccgg
     7621 gtttatccat ttctctgaat tgatatctga aatagtttca gttaatatcc attcaagatc
     7681 ttttaaaaaa ttatcttttt caaattcatc tttaagctct tcataaattt cagaagtaga
     7741 taaaattact tttgcaccac tgtcttttgc aattgcagca attctcgata atgatttatt
     7801 ttttccggga gggtgaagtg gtacagaaat tactcctgca taaaggcatc caaaaaatga
     7861 atctaaatat tcaagtccgg attcataaat tagtaatgct ctttcaccat ttaaattttt
     7921 tatctgtaaa ctttcggcaa ttctttttgc actgatatca agtgccttgt aagaaaattt
     7981 acctgcttca gtttccccgt cttccagaaa aacaaatgca gttttatccg gttgtttttg
     8041 agcgcgatat attaataaat caactaaatt ttgagctttt cccaatgaaa tgtttgtata
     8101 attaagacaa caatataaaa attctaattc tgtaattaaa tgactatata agggatattt
     8161 agttgtaatc tgatttaatt ggcaataatg atcagaatta atttctaagc gtattaaaaa
     8221 ttaatgaagt gatcctgaga atatgaatat tataaaaaca ttgtattgcg ataattgatc
     8281 taaaaattta ttttaaatat actcaataaa aggtaaatca ttaaaccata aaatgacatc
     8341 aaaagcaaag tcaattgcag aatatttaga tgcacttcct tcagaaagaa aaacagcagt
     8401 aagtaaactt caaaagtgta ttaaaaaaaa acttcctaaa ggttttaagg aagaaatatc
     8461 ttatggaatg ataggttatg tcgttcctca aagtctgtat ccggatggat atcactgcaa
     8521 ccctgagctg ccgttgcctt tcatgagtat tgcctctcaa aaaaatttta ttgctcttta
     8581 tcatatggga ttatatgctg ataaaaaact acttgactgg tttacaaatg agtacagtaa
     8641 aacagttaaa tcaaaattgg atatggggaa aagctgtatc agatttaaaa agcctgaaga
     8701 gattccatat aagctaatcg gtgaacttgc ttcaaaggtc tctcctcaag actggatcag
     8761 tacttacgaa aatattttaa agaaaaaata atatatccct ttttaaaaaa tagaatgcaa
     8821 agaaatggtt tttgaaaaaa tttacaactt tgaacgaaat aaatataatt taaatggttt
     8881 tcataaaaat aaaattttat acctttttat agattttatt aaggaatgtg aagaagattt
     8941 ctttgatgaa tttgatccat attttgccaa tgctttttat gcaaacagca gtgtattgct
     9001 tttattaaaa tcatgctttt gcactgaaga taatgaaatt tttggaatgg aaagtaaaga
     9061 tggtgagatt gatattgaaa tgaatatgga aatggaagaa ttcagcgaga ataaaactat
     9121 atattctctg agcagttcta tcaaaggaaa agaagatgaa cacttgcaca tgattattga
     9181 taatatatat tcagactctc aatttgcttt gaaatatgta cctgaagatg aagaagatga
     9241 tgaaaatatg gagttcatca cagatcccga atgtaaatta tctttgcttg tctgatgtaa
     9301 tttaaatcat attaagaaaa gatcatttga tttttaattc aagtctgtcg agtcctcttg
     9361 agtgaagtga ttcatgaaaa ttatattctt tatccatcag actcaaacgc ggaaatcgtt
     9421 tagcaatatt ttcaaatgct gaaacagctt cgagttttgc aaaacctgca ccggcacaaa
     9481 aatgaatgcc acctccaaat gaaaggggga atactttttt cctttcaata tcaagtctgt
     9541 caggctctgg aaatttcaga ggatcacgat ttcctgctcc cagcatacag ataatctgca
     9601 gacctttttt aatttccttt cctccgactg tttcatcttc atgtgtcctc ctgcctgtaa
     9661 actgtgcagg gctgtcatat cttaaaagtt catctgcagc tccgtcggca agtgaaggat
     9721 ctttcacaag cttttcatac tgattgggat tttttaacaa ggcataaagt ccgtttgaaa
     9781 tctgattctt tggagcttca tgcgcatcca ttatcatcaa aacaagagaa gcaattatct
     9841 cttcttttgt gagcttatct ccgttgtctt caatagtaat caatctgctg atgagatcat
     9901 ctttcggatt tttttctttc tgatctacaa cttttctaaa atattctttt atctcatctg
     9961 ctgagctgta aatatttttc agatcttcct gtgtaaattg atttgtaact acatctaaag
    10021 ttcttactat ctgcattgcc catttatcta aaatatctct gtcctctttt ggaatcccgt
    10081 aaaattcagc aattgtaata gcagcaaggg gatatgaaaa ttcacttatg aattcaatag
    10141 tatctccctg aaatttatca agcagataat ttacaatgga gtcaatattt ggtttcagaa
    10201 cattcactaa ttttggcgaa aaagctttgt tgataaatcc tctgatccgg gtatgttcag
    10261 gcgggtcaag aaaatttacc caatgttgtc tcatttgagt aagtgaattc agtcttgcat
    10321 aatttgcagt aatttttcct ttcccaaact tatttcctct tccaaatttt ttattaatta
    10381 gaacggatga aatatgatca tatctcgtaa gtatccagat attttctgac aaaaaacctg
    10441 ttggggattt catataaaat acaggctcat aatctctaag cttttgatat acaggatatg
    10501 gatcctgaat attttctttt aaagaaagtg ctttaaataa attaaaattt tcgtcaatcg
    10561 tcagttcgtt ttccaaagtt gtattgatca ttttaaaaaa atagaaccta aactaattta
    10621 attaggaaag ttattcaaga cagttacaaa ctacaaatta caaaatacaa attacgaata
    10681 ataaaaaacg aattataaat tataaattaa tctgttacaa cctaaattaa tcgcaaactg
    10741 atttaaaatt ttcagtgaaa cttgttaagg tatatgtcaa aattttaaca gcacgtttca
    10801 aaataattta aaagtaattc caccttaaaa atgatggagc gaaaaagttt atacggaaag
    10861 aaacagtaac aacagaatgt aataaactta acatcaaaaa aagtagagta aattaagctt
    10921 aagtttacaa acccttgaga agaatagtta taaaatatct agttttattt taaggcttaa
    10981 atttcttaat ttaaattatc g
//
LOCUS       DAORVQ010000107        10938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3073_length_10938_cov_5.008086, whole genome shotgun sequence.
ACCESSION   DAORVQ010000107 DAORVQ010000000
VERSION     DAORVQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_10938_cov_5.008086"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..2473
                     /locus_tag="PLX80_09425"
     CDS             41..2473
                     /locus_tag="PLX80_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY21048.1"
                     /translation="MKNVDLFLKLTAIFIFFGNSANSQLYWNQACTFSGSANSYVSVP
                     NSLSLNLTSDFTFEAWVNPELTGISSKGIIAKGGTLGTSLRYGLRLLNTGRINLFTNG
                     VQRLTSKSSTLIQSNEWTHISASYESASNTFKIYVNGVLDTSSAVPGASPSTNTDSLY
                     IGISGNSTPYKGQLDEVRIWNRCLSSNEISEYFRTSLGTNSGIYFGLNLSLTFQKRSN
                     ISPFSLTDQSGKDNNASGFNITASNMTFQPYNTISTNEAINLNGTNDYLSAKDSSMMF
                     PFNKITIECWVYPRDSNMSVVIKKQSGINTAFSLAVSSTEIRTTINNNVFTRAATIPQ
                     NQWSHIAISYSLSAGLLTTFLNGNRIGTQSASAGSISNTSDSVFVGGAPGQSEFFNGY
                     IDELRIYAIFHDDLDIIRNMYRGIDASIKNEISSILFSYKFDGNTFDNAESGGPKLFL
                     RNRAKFSHPSANFNTPRSPLLSGQNNNYYKSYYFLPVNKKIPETGSAGIVLSSMKINM
                     SKPINDVDVFVNLEHASSSDLRITLIRSESSDSILLFDGKITNSSDNNISLIFDDQAD
                     STLGNNKYSSFCSGIKPLNNLNSFFSGMDSKAMWTLNVNDNVSGNTGFIYSWGIRFNN
                     LTTPQTNLTLSSFIQGFYNPISNLTVTDTLTVELRSVVSPYALIQGSKAVLEINGICP
                     VSFDNLDYDFLHYIVLKHRNSIETWSDNFVIFSNQFQAVQNFNLDSSFAFGDNQIRVD
                     DSPVKYAIYSGDVNQDDAVDVSDLSLIDNDAFNFASGYIPSDVNGDDVTDLSDGLITE
                     NNAVNFVTKISP"
     gene            2725..3375
                     /locus_tag="PLX80_09430"
     CDS             2725..3375
                     /locus_tag="PLX80_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQY21049.1"
                     /translation="MRTSLEDYLKNIYSLQTTDGKVTNMILAEKMNISPAAVSDMISK
                     ISKSGYIKNTPYKGFELTKKGEVIAINLVRKHRIWEVFLVKDLAYPWEKVHSEAENLE
                     HASSDELISRLEAFLGNPKFDPHGHPIPDKNGKIPVSNLTCLSELKVGDKAVIRQVSD
                     ESSEVLQYLSKAGLKLNDKIEIIEKISFDNSIQIKLKSKNIFLSEKLSKNIFITKN"
     gene            3376..4275
                     /locus_tag="PLX80_09435"
     CDS             3376..4275
                     /locus_tag="PLX80_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQY21050.1"
                     /translation="MKNLNLLLVAVIIICSGCKNDNSTEEVSSGKIEAVSSINIISDI
                     VQNIGGDKVDSYSICGVGVDPHTYQPKPSDPRKVSKSKIVFINGFNLEHWIEEMIKGA
                     GGEKKVVTVSEGITPMTDEKGFGDPDPHAWFDVANVKTYAENIAIGLKEIDPENAEYY
                     EANLKKYLSELESLDNWIKSEITKIPEDKRILITSHDAFRYFGRAYGLEVKGLQGIST
                     EAKVRTEDFKNIIDLIKEKKIRSVFIETSVNPKLLEQISDETGAQVGGTLYSDSIGNE
                     GTDEGSYIGAVKFNVNTIVNSLK"
     gene            4278..5102
                     /locus_tag="PLX80_09440"
     CDS             4278..5102
                     /locus_tag="PLX80_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQY21051.1"
                     /translation="MKRRTKINLSILQFLLIGNGILFSQELPPIVTDRPDITESAETV
                     QKNRLQIESGLLYNRDQGSYYDSLDQVVNFANEFITLPNVLFRYGINDFMELRLALSV
                     TINNEDYNGKYENSSITNFDAPSFGLKFKLGKQDGIEPSAAVIIQAVSPVLSTESEDD
                     YFDPSIIFCFQNTVSDNFSLGYNAGLNLDDGFGQGDGIFLSLSGSYSFSEKVSAFGEI
                     FSSTNFYYLVPNSGADIGVTYLVSNNIQLDLSAGASLENIKDDYFFGTGFSIRIPN"
     gene            5107..5853
                     /locus_tag="PLX80_09445"
     CDS             5107..5853
                     /locus_tag="PLX80_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HQY21052.1"
                     /translation="MNVIKVKNLTIAYKKRPAIKGINLNIEEGNIIGIIGPNGAGKST
                     LLKGILGLLPIETGEVKIFDKPVKESLQRLAYIPQKEQFDWDFPINVSEVVMMGRYPH
                     LSLFGKPKKEDHEIVKEVLKKVEMTEHGNTQIRNLSGGQQQRIFIARALAQESDVYFL
                     DEPFVGVDAKTEKAIFELIRELKEKNKTILIVHHDLSKVSEYFDKVIMINQTLIAFGD
                     TEKVFTADIINKTYGGRLTILQKAEQLINR"
     gene            5858..7135
                     /locus_tag="PLX80_09450"
     CDS             5858..7135
                     /locus_tag="PLX80_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HQY21053.1"
                     /translation="METITKFLIEPLQYEFIQRALVASIMVGISCGLIGTYIMLRRLS
                     LIGDALAHSVLPGVVIGFMISGKNPLGLFLGALVAGILTSILISYVERNSKIKEDTSI
                     GIIFTGAFALGILLVSQLKQVHIDLSSYLFGDVLGVSDSDLIMSSVITVFILISVILF
                     YKQLLVTSFDPVMAYIIGISTSLVHYFLMTLLSMSIVAGLQSVGVILIIAMLITPPAT
                     AFLLTDKLKPLLILSVLIGVFSSVIGLYLSYHFNFASGASIVLTAVVIFSLTFLFSPK
                     EGVVQKYFRRKRNSGINLTEDILKYLFNAEEKHIEEDLFSKLSLELGLSQNKIISALK
                     RTERNGLISGDKNNYKLTEKGRELATKLVRSHRLWETYLTEKNIVDMDSIHQDAEKYE
                     HILSDDLIAEIDEELGHPQKDPHGSPIPKKKEG"
     gene            complement(7324..8208)
                     /locus_tag="PLX80_09455"
     CDS             complement(7324..8208)
                     /locus_tag="PLX80_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21054.1"
                     /translation="MVAIIPLWIEGYKKSISDLSKEDEKLLRTISSSTIDRILSKFRS
                     KYQKKGLCTTRPGSIIRDLIPIKTDQWDEWRPGYIEMDLVAHCGTSVAGEYINTLNVV
                     DIATGWVSQRAVWGKGEHNTFKAIREIELTLPFQIKGIDSDNGKEFMNYRLLKYFKHR
                     KEPVNYTRSRAYNKNDNSHIEEKNWTKIRQYIGYDRLDNPEQLELLNDLYRNEFNDFN
                     NFFLASMKLKTKERIGSKIKRKHEEPKTLFQKLYESKDIEDKKKLELHRKRNKLNPFE
                     LQEKIAKKINTILKMNFK"
     gene            complement(8212..8481)
                     /locus_tag="PLX80_09460"
     CDS             complement(8212..8481)
                     /locus_tag="PLX80_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21055.1"
                     /translation="MSQNSKKEYLKEIAIRYRKSDKEGKKKILDEYCKVCNYNRKYAI
                     RKLNQFLSERKNRKNKRAGRKKKYYTEGVIEFIKTLWIKSNLICS"
     gene            8660..10171
                     /locus_tag="PLX80_09465"
     CDS             8660..10171
                     /locus_tag="PLX80_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21056.1"
                     /translation="MKASTKIFLALIFYTSLSTFFFQNSFAQLVSDFHVNEDSTSYSQ
                     YAAKVGSDQNGNFVIVWYDSRTSENPRISAQRYNQKAQKIGNNFQINNDQYQAFPSLH
                     VRENGSFIVAWSVVQTKFKIFNSSGLPITNEIIISDSSGMISSIGGDSYGNFVIVWEQ
                     YNSALKYQIYCQLIDSSGKLIGSKIKVNDDNLDIHHKNPDITIRNDGSFIVTWQDSRP
                     PSVQNSEDIYMQMFSKKGTPIGNNIKVSDDTVRYNKQSEAKISSDTSGRFCISFTEFI
                     LNEVQSNIVCQLYHPDGSRQGNNFNIAGSIISEFGGVIFKKENGDMVSGYSYYSNRYE
                     PYFQRINSQGNLTGNGYLVSTEFSFADKYFNDIAVWDDRIISVWQDKRNGNYDIYCNI
                     RSYSNPDSTVNIQNISTEIPNEFKLFQNYPNPFNNTTQIRFDIYTNDQYSMSVYNNLG
                     QKIKEIFKNYLSPGTYKISFESGELSSGVYHYILSSPNKIRKKRLVKSFVLIK"
     gene            10219..>10938
                     /locus_tag="PLX80_09470"
     CDS             10219..>10938
                     /locus_tag="PLX80_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21057.1"
                     /translation="MKSLFYFLVLVFFSVNLSFSQEPTQCLHTENIIQDTIPNYLQPW
                     WNQIRTGKLAVVYVDFPDGRWNDNGVLRQPYTNQQLDSVPNKDAAGEVGVIKNTSGQF
                     EMQASKYSYYDRWNLLFDSLGVYNGTAHPDFLSHGDSAYGSMKQYWEEASNGKFDLIP
                     YQTRTPDPNIDNRLWRGIINDYEMVNGIPVIKNILLPKNKYGTDEAFSYFRSYEDTDS
                     LSNKGVDIIDDTKIAIQSIFDL"
BASE COUNT         3695 a         1683 c         1890 g         3670 t
ORIGIN      
        1 taaaaagatt ttaattttta taaatcaaat aaaaatgaaa atgaaaaatg tagatttatt
       61 tttgaaatta actgcaatat ttattttttt tggaaattca gcaaattcgc agctatactg
      121 gaatcaggca tgtacttttt caggatctgc taacagttat gtttctgttc caaactcttt
      181 atctttaaac cttacttctg attttacatt cgaagcatgg gtaaatcctg aattaaccgg
      241 aatttcatca aaaggaatta ttgcgaaagg agggacttta ggaacttcac tgagatatgg
      301 acttagacta ctcaatacag ggagaataaa tctcttcaca aatggagttc aaagacttac
      361 ctctaaaagc tcaacactta ttcaaagtaa tgaatggact catattagtg cttcatacga
      421 atcagcatcg aatacattta agatatatgt caacggtgtt ttggatacat catcagctgt
      481 tccgggtgcc tcaccatcta caaatactga ttctttatac attggaatat caggaaattc
      541 tactccctac aaaggtcagc ttgatgaagt aaggatctgg aacaggtgtt taagttcaaa
      601 tgaaatttct gaatatttta gaacttcact cggcactaac agcggaattt atttcggttt
      661 gaatctatcc ctgacttttc agaaaagaag taatatttct ccgttttcat tgacagatca
      721 atccggaaaa gataataacg caagcggatt taatataacc gcatctaata tgacttttca
      781 accttataat actatcagca caaacgaagc tataaattta aatggtacta atgattatct
      841 ttcagcaaaa gacagttcca tgatgtttcc ttttaataaa ataacaatag aatgctgggt
      901 atatcccaga gacagcaata tgtctgttgt aataaaaaaa cagtcaggta taaatactgc
      961 tttttcttta gcagtctcgt caacagaaat cagaacaact attaataata atgtatttac
     1021 aagagctgca acaattcctc aaaatcagtg gagtcatatt gctatttcat acagtttatc
     1081 tgctggactt ttaacaactt ttttaaatgg caatagaatt gggactcagt ctgcttctgc
     1141 cggaagtatt tcaaatacaa gtgattcagt ttttgtagga ggtgcacccg gtcaatctga
     1201 gttttttaac ggatatattg atgagttgag aatctatgcc atttttcatg atgatcttga
     1261 tattatcaga aatatgtaca gaggaattga tgcttcaata aaaaatgaaa tatcatcaat
     1321 tttattctcg tataagtttg acggtaatac ttttgataat gctgaaagcg gtggtccgaa
     1381 attatttctt agaaatagag ctaagttcag tcatccatct gcaaatttta acacaccaag
     1441 atcaccattg ttaagtggtc agaataacaa ttattataaa tcatattatt ttttaccggt
     1501 aaataaaaag atccctgaga caggctcggc gggaatagtt ttaagttcaa tgaaaataaa
     1561 tatgtcaaaa cctataaacg atgttgatgt atttgtaaat ttagaacatg caagctcttc
     1621 agatctgaga attactttga taaggtcaga atcatcagat tctattttac tttttgacgg
     1681 aaaaattaca aacagtagtg acaataatat ttcactaata tttgatgatc aggctgacag
     1741 tacacttggc aataataaat actcttcgtt ttgttcaggg ataaaaccgc ttaataatct
     1801 caattcattt ttttcaggca tggattcaaa agctatgtgg acattaaatg ttaatgataa
     1861 tgtatcgggg aataccggat ttatttattc atggggaata agatttaaca acctgacaac
     1921 tccgcaaacg aacttaacat tgtcttcatt tatccagggc ttttataatc caatttcaaa
     1981 tctgacggta acagatacat taactgtaga attaagatcc gtagtatctc cttatgcact
     2041 gatacaaggg tctaaagcag ttttagaaat caacggaatt tgtccggtta gttttgataa
     2101 tttagattat gactttttac attatatagt tttaaagcac agaaattcta ttgaaacatg
     2161 gtcagataat tttgttattt tctcgaatca gtttcaggct gtccagaatt ttaatttgga
     2221 ttcatcattt gcttttggtg ataatcagat cagagtcgac gattctcctg taaaatatgc
     2281 aatttacagc ggagatgtaa atcaggatga tgctgtagat gtgtcagatc ttagtcttat
     2341 tgataatgat gctttcaatt ttgcaagcgg atatattcct tcagacgtta acggcgatga
     2401 tgtaacggat ctttcagatg gtttaattac agaaaataat gcagtcaatt ttgtaactaa
     2461 aatttctccc tgatcaatat ttatctaaat aactttcact catataaata tgaaaacagt
     2521 atttggattt cttttattga aaaatttcta gagttattta agcaattccg ataatattgc
     2581 aatcctatcc ctttcaaaag aaagctgctt atatttaata cttaggcagc ttttactata
     2641 actgtaaaac acggtataaa atatatttaa attaacttgc atataaaatt aagcatgctt
     2701 aactttatgc aaataaattt acacatgcgg acaagcttag aagattattt aaaaaatata
     2761 tattcattac agacaactga cggtaaagta actaatatga tacttgctga aaaaatgaat
     2821 atctctcctg cagcagtaag tgacatgatc tcaaagatct caaaatccgg atatataaaa
     2881 aacactccat ataaaggatt tgagcttaca aagaaaggtg aagtaattgc tataaatctt
     2941 gtgagaaaac accggatatg ggaagtcttt ttagtaaagg atctggctta tccctgggag
     3001 aaggttcatt cggaagctga gaatcttgag catgcttcat ctgatgaatt gatttccaga
     3061 ctggaagcat ttcttggaaa tccaaaattt gatccacacg gtcatccgat ccctgataag
     3121 aacggtaaga ttccagtatc taatcttact tgcttgtctg aactaaaagt tggggataaa
     3181 gctgtgataa ggcaggtctc tgatgaaagt tcagaagtgc ttcaatattt atctaaagcc
     3241 ggtctaaaac taaatgataa gatcgaaata attgaaaaaa tatcttttga caattcaata
     3301 cagatcaaac taaagtcgaa aaatattttc ctaagtgaaa aattatcaaa gaatatattc
     3361 attacaaaaa attaaatgaa aaatttaaat ctattattag tagcagttat aataatctgt
     3421 tcaggttgta aaaatgataa cagtactgag gaagtttcca gcggaaaaat tgaagcagtc
     3481 tcatcaataa atatcatcag cgatatagtt caaaatatag gcggtgataa agtcgattca
     3541 tacagcattt gcggagtcgg tgtagatcct cacacttacc aacctaagcc gtccgatccg
     3601 agaaaggtga gtaaaagtaa aattgtcttt ataaatggtt ttaatctgga gcactggata
     3661 gaggaaatga tcaaaggagc cggcggtgaa aaaaaagttg ttactgtatc tgaaggaatt
     3721 actcctatga cagatgaaaa agggtttgga gatccggatc cgcatgcatg gtttgatgtt
     3781 gcaaatgtaa aaacatatgc tgaaaatatt gcaattggtc taaaggaaat tgatccggaa
     3841 aatgctgaat actatgaagc aaatttgaaa aaatatcttt ctgaattaga gtcattagac
     3901 aactggatta agtctgagat tacaaagatc cccgaagata agagaatact cattacatca
     3961 catgatgcat tcagatattt cggcagagca tatggactgg aagtaaaagg tcttcaggga
     4021 atttccacag aagcaaaagt caggacagaa gattttaaaa atattataga tctgataaaa
     4081 gaaaaaaaga tcagatcggt ttttattgaa acaagtgtaa atccaaaact gctcgaacag
     4141 atctcggatg agactggcgc acaggtcggc ggaacgcttt attctgattc cataggcaat
     4201 gaaggaactg atgaaggcag ctatatcgga gcagtaaaat ttaatgtgaa tacaatcgta
     4261 aattcattaa aataaatatg aaaagaagaa ctaaaataaa tttatccatt ttacaatttc
     4321 tgttaattgg aaacggaatt ttattttctc aggaattgcc tccaatagtt acggacagac
     4381 ctgatatcac tgagtctgca gagactgttc aaaaaaacag attgcagatc gagtctggtt
     4441 tgttatataa cagagatcag ggttcatatt atgattctct tgatcaggtt gtaaattttg
     4501 caaatgaatt tattactttg ccaaatgtat tgttccgcta tggaataaat gacttcatgg
     4561 aattgagact tgcgttgtca gttacaataa ataatgaaga ttataatggc aagtatgaaa
     4621 atagcagtat tactaatttt gatgctcctt cttttgggct taaatttaaa ctgggaaagc
     4681 aggacggtat agagccttca gctgcagtca tcattcaggc agtcagtccg gtattgagta
     4741 cagaatcgga agatgattat tttgatcctt caattatatt ttgttttcaa aacactgtat
     4801 cagataattt ttcattagga tataatgccg gtctaaactt agatgatggt tttggccagg
     4861 gggacggaat ttttctttcg ttgtcaggaa gttattcttt ctcagaaaaa gtttcagctt
     4921 ttggagagat atttagttca actaattttt attacctggt accaaacagc ggtgcagata
     4981 ttggtgttac atacttagtc agtaataata ttcagcttga cctatctgcc ggtgcaagtt
     5041 tagaaaatat aaaagatgat tatttcttcg gcacgggatt ttcaatcaga attccaaatt
     5101 aaaattatga atgtaatcaa agttaaaaat cttacaattg cctataaaaa aagacctgcg
     5161 ataaagggaa taaatcttaa tatcgaagag ggaaatatca ttggcattat cggtccaaac
     5221 ggagcgggca aatctacatt gcttaaaggc atactgggat tgctgccgat cgagaccggt
     5281 gaagtgaaga tctttgacaa acctgtaaag gaatctttgc aaaggctggc ttacattcct
     5341 cagaaggaac agtttgactg ggattttccg atcaatgtat ccgaagtggt aatgatggga
     5401 agatatccgc atctcagtct ttttggaaaa ccgaaaaaag aagatcatga aattgtaaaa
     5461 gaagttctga aaaaagtaga aatgacagaa catggaaaca cacagatcag aaatctttca
     5521 ggcggtcagc agcagaggat atttatagcc cgtgcacttg cacaggaaag cgatgtttat
     5581 tttctggacg aaccttttgt tggtgtcgat gcaaagacag agaaagctat ttttgaattg
     5641 atcagagaac ttaaagaaaa aaataaaacg atcttaattg ttcatcatga cctgagcaaa
     5701 gtcagtgaat attttgacaa agtcattatg ataaatcaga ctctgattgc atttggtgat
     5761 actgaaaaag tatttactgc agatataata aataaaacct atggcggcag acttacaatt
     5821 ttacaaaaag ctgaacaatt aataaacaga tgacaacatg gaaactataa caaaattttt
     5881 aatcgaaccg ctgcagtatg aattcataca gcgcgcactt gtagcctcga taatggtggg
     5941 gataagctgc gggctgatcg gtacatacat tatgctgaga agactatcgc tgatcggtga
     6001 tgcactggca cattcagtat tgccgggagt tgtgatcggg tttatgatct cggggaaaaa
     6061 tccgctgggt ttatttttag gagctttggt agccggaata ttgacttcaa ttctgatcag
     6121 ctatgttgaa agaaattcga agataaaaga agatacttcg atcggcatta ttttcacggg
     6181 agcatttgct ctgggaatat tacttgtgtc acagcttaag caggttcaca tagatctgtc
     6241 ttcatattta ttcggtgatg tacttggagt ttcggattca gatctgatca tgtcatctgt
     6301 gatcacagta tttattttaa tatcagtgat tttattttac aaacagcttt tagtcacttc
     6361 attcgatccg gtcatggctt atataatcgg catatcgact tcacttgtgc attatttttt
     6421 gatgacgctg ttatcgatgt cgattgttgc aggactgcag tcagttggag ttattctaat
     6481 aattgcgatg ctgatcacac ctccggctac ggcttttctt ttgacggata aattaaaacc
     6541 gttactgata ctttcagttc tgatcggagt attctcatca gtcattggat tatatttatc
     6601 ttatcatttt aattttgcat ccggagcttc tattgtatta acagcagttg tgatcttttc
     6661 actaacattt cttttctcac ctaaggaagg tgtagtacaa aagtatttca gaaggaaaag
     6721 aaattcgggt atcaatctta cagaagatat tttgaaatat ttattcaatg ccgaggaaaa
     6781 gcatattgag gaagacttgt tttcaaaact ttccttagag cttggtttat cacagaataa
     6841 aattatatca gctttaaaaa gaactgaaag aaacggatta atatcgggtg ataaaaataa
     6901 ttataagctg acggaaaaag gcagggagct tgcgacaaaa cttgtcagat cacacagatt
     6961 atgggagaca tatcttactg agaaaaatat tgttgacatg gacagtattc atcaggatgc
     7021 agaaaaatat gaacatatat tatcagatga tctgatagca gaaatagatg aagagcttgg
     7081 tcatccgcag aaagatccgc atgggtcgcc gattccgaag aagaaggagg gttgataaat
     7141 ttataattta ctgaaatttc tatcgagtca tttctataac atttttaaag cgttttcaca
     7201 gaaaccagtt gcatcataat aaaaggtaac cgtagcagta gtaatctttt cttttctttt
     7261 ttgcttcttt tttcttttct tttgttaata atgttagtat tgttaatatc tttatttcca
     7321 tttttatttg aaattcattt ttaaaattgt gtttattttt tttgcaattt tttcttgtaa
     7381 ctcaaaagga tttaatttat ttcttttcct atgcagctct aatttttttt tatcctcaat
     7441 atcttttgat tcatataatt tctgaaatag tgttttaggc tcttcatgtt ttctcttaat
     7501 ctttgaacct attctttcct ttgtctttaa tttcattgac gctaaaaaga aattattaaa
     7561 atcattaaat tcatttctat aaagatcatt taataattca agttgttccg gattgtctaa
     7621 tctgtcatat ccaatatatt gtctgatctt tgtccagttt ttttcttcta tatgtgaatt
     7681 atcattttta ttataagctc ttgagcgtgt ataatttact ggttcttttc tatgcttaaa
     7741 atattttaac aatctgtaat tcataaattc ttttccatta tcactgtcta ttccttttat
     7801 ttgaaatggc aatgtcagtt ctatttctct aatggctttg aatgtgttat gttctccctt
     7861 tccccagact gcccgctggg aaacccagcc tgttgctata tctacaacgt ttaacgtatt
     7921 tatatattct ccggctacgc ttgtaccgca gtgagctact aagtccattt ctatatatcc
     7981 gggtctccat tcatcccatt gatctgtctt tattggaatc agatctcgta ttattgagcc
     8041 tggtcgggtt gtacataaac ctttcttttg atatttgctt ctaaacttcg atagtatcct
     8101 gtctattgtt gatgatgaaa tagttctcag taatttttca tcttctttac ttaaatcgga
     8161 tatagatttt ttatatcctt ctatccataa tggtattatt gctaccattc gttatgaaca
     8221 aatcaaattt gattttatcc aaagagtctt tataaactct ataactcctt ctgtataata
     8281 tttctttttg cgaccagctc ttttattctt tctatttttt ctctcgctta aaaactgatt
     8341 caattttctt attgcatact ttcggttata gttacatact ttacagtact catcaagtat
     8401 ttttttcttt ccttctttat ctgatttgcg atatcgtatt gcaatctctt tcagatattc
     8461 tttttttgaa ttttggctca taattttacc gttttttatt ctttaacaac ggttacatta
     8521 attatgatgc aacgaccact aattgaatca cccttgggtt acattaatta tgattcaatg
     8581 cggaaaagtt ttgttttaat aaaatgatta gtttgttaaa tttgttttat ttacattgca
     8641 ataattaata tccaaagata tgaaggcttc aacaaaaata tttcttgcat taatttttta
     8701 cacatctcta agtactttct tttttcaaaa ttcttttgcc cagttggttt cggattttca
     8761 tgtgaatgaa gatagtacca gttatagtca atatgcagct aaagttggta gtgatcaaaa
     8821 tggtaacttt gtaatagttt ggtatgatag tagaacatct gaaaatccga ggatttctgc
     8881 tcaaagatat aatcaaaaag ctcaaaaaat tggaaataat tttcaaataa ataatgatca
     8941 ataccaggct ttcccttctt tacatgttag agaaaacgga agttttatag tggcatggag
     9001 tgtcgtacaa acaaagttta aaatatttaa ttcttctgga ctgcccataa ccaatgaaat
     9061 aattatttcg gattcatccg gtatgatttc ttctatagga ggagattcat atggtaattt
     9121 tgtaatagtt tgggaacaat ataattcagc tttaaaatat cagatatatt gtcaattaat
     9181 tgattcgtca ggaaaattaa tcggaagtaa aataaaagtt aatgatgaca atttagatat
     9241 tcatcataaa aatcccgata tcacaatcag aaatgacgga agttttattg tcacctggca
     9301 agattcccgt ccgccatctg ttcaaaactc ggaagatatt tatatgcaga tgttttcaaa
     9361 aaaaggcaca cctattggaa acaatattaa agtcagtgat gatacagtaa ggtataataa
     9421 acaatctgag gcgaaaatat cttccgatac atcaggaaga ttttgtatct catttactga
     9481 atttattttg aatgaagttc aatcgaatat tgtatgtcaa ctttaccatc ctgacggatc
     9541 aagacaagga aataacttta atattgccgg aagtataata agtgaattcg gaggagtgat
     9601 ttttaaaaaa gaaaacggtg atatggtatc cggttatagt tattacagca atagatatga
     9661 gccttatttt cagagaataa acagtcaggg aaatttaact ggtaatgggt atttagtatc
     9721 cacagaattt tcttttgcag ataaatattt taatgacatt gcagtctggg atgacaggat
     9781 aatcagtgta tggcaggata aaagaaacgg taactatgat atatattgca acatccgttc
     9841 atattcaaat cctgattcaa cagtgaacat tcagaatata tcaacagaga ttccaaatga
     9901 atttaaatta tttcagaatt atccaaatcc gtttaataac acaacgcaaa tccgatttga
     9961 tatttatact aatgatcaat acagtatgtc agtttataat aaccttggtc agaaaataaa
    10021 agaaattttt aaaaattact tatcacccgg cacatataaa attagctttg agtcaggtga
    10081 attaagttct ggagtttatc attacattct ttcttccccc aacaaaataa gaaagaaaag
    10141 acttgtgaaa agttttgtac ttataaaata aaaaattttc tcatttgaac atccgcagag
    10201 cggaaggagg aatcaaaaat gaaatcactc ttttattttt tagttttagt atttttttca
    10261 gttaacctat cattttctca agaaccaact caatgtcttc acacagagaa cattattcag
    10321 gacacaattc caaattacct gcagccatgg tggaatcaaa taagaacagg gaaattagca
    10381 gttgtttatg tagatttccc tgacggcaga tggaatgata atggagtttt aagacaacct
    10441 tatactaatc agcaattaga ttcagtacca aataaagatg cagccggcga ggtcggagta
    10501 attaaaaaca cgagcggaca atttgaaatg caggcatcta aatacagtta ttatgacaga
    10561 tggaacttat tgttcgactc acttggagtt tataatggta ctgctcatcc tgatttttta
    10621 tctcatggag attcagctta cggtagtatg aagcaatact gggaagaagc ttcaaatgga
    10681 aaatttgatt taattcctta tcaaacaaga acacctgatc ctaatattga taacaggtta
    10741 tggagaggaa tcattaatga ctatgaaatg gtcaatggaa ttcctgtaat aaaaaatata
    10801 ttgcttccta aaaataaata tggaacagat gaagcatttt catactttag atcttatgaa
    10861 gatacagatt cattatcaaa taaaggagtt gatataatag atgatactaa aattgctata
    10921 caatctattt ttgattta
//
LOCUS       DAORVQ010000108        10930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3074_length_10930_cov_6.823724, whole genome shotgun sequence.
ACCESSION   DAORVQ010000108 DAORVQ010000000
VERSION     DAORVQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_10930_cov_6.823724"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /gene="radC"
                     /locus_tag="PLX80_09475"
     CDS             <1..443
                     /gene="radC"
                     /locus_tag="PLX80_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQY21058.1"
                     /translation="TKKIIKELKEEENTLRDNKKKYNPVTNPEALFNILVSKLTDYSK
                     EHFYVISLDSRNIFIGLDEISVGTLTASLVHPRETFESAIKRHAAHIIIAHNHPSGET
                     SPSEDDLKITRRLIDAGKIMGITVLDHIIFAKHSYMSFKESSLI"
     gene            complement(469..651)
                     /locus_tag="PLX80_09480"
     CDS             complement(469..651)
                     /locus_tag="PLX80_09480"
                     /inference="COORDINATES: protein motif:HMM:NF020006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HQY21059.1"
                     /translation="MEYTIVTADSNSELVKKVNEMLNQGWETEGGVAISQDGNFHQAM
                     ILFDDVNEEFADGEEL"
     gene            1081..1335
                     /locus_tag="PLX80_09485"
     CDS             1081..1335
                     /locus_tag="PLX80_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21060.1"
                     /translation="MTQAEMQTEFSRLVNRGIIFGSIWIFGIGSIIAFTSGFQAIKLY
                     KQSDKSLSGKNKIFKCFLIGAIGLLLDLTAVLIIILFRKK"
     gene            1348..2298
                     /locus_tag="PLX80_09490"
     CDS             1348..2298
                     /locus_tag="PLX80_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQY21061.1"
                     /translation="MEYKDYYNVLGVKKTATQDEIKKSYRKLALKYHPDKNKDNKEAE
                     EKFKEINEANEVLSDKDKRKKYDELGENWKYHQQSGGAQDQGFDWSQYANQGSGYTQY
                     SYGQDQGDSFGGSNFSDFFETLFGGGGGFSSSGRGGRSRAARANMRGEDLSAEMSISL
                     EDAYKGTEKMFELSGQSIKLKIKPGIASGQTLRLSGKGGKGVSGGSSGDLLLTIHVLQ
                     DPVYERKSDDLYTDVKVDLYTAVLGGKATLKTFKGNINLNIPKEAQSGKVLRLQGLGM
                     PKYGKVNEFGDMYAKIIVETPVNLNERELKLFQELSDMRK"
     gene            complement(2358..3809)
                     /locus_tag="PLX80_09495"
     CDS             complement(2358..3809)
                     /locus_tag="PLX80_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21062.1"
                     /translation="MNFRKLVFSIFLLCIITSISYSQNPNFELKLTNRNYLSSNLGYF
                     DVVLKHTNFLQTEFRYSSGKFAIILDQSFLPDHLNYYYSKDTSTFGRRRISPNNVGDL
                     IRSGDTLFINEGNIITQGTEPIISHEEGTLIARIRFFNPDLYLNSCLFEEQYVWKLNN
                     SGLTTEIKANVSGSIVSLQNSLNNFSTNDLCDDQFCCLSVPLTPPVRIFPLNNSINNF
                     WPVKFEWQKGFPVNYSANFQVATDSLFSDILIDEDIERPDGIDPVKFFTSDLEFETDY
                     YWRVKQGGIPPQGAYNESWKFTTGTPSITLSLKVIPESLINKFNFYPFKLKVYLRNSS
                     SPYEIIDSSQAFIKNHNFNTELYFMNAVSGNYYIVLDNGKYLETWSRSGGSFLISQNL
                     NLYDFSSHSSQAFGNNQTSVGDLNCIFSGDLNNDGLVDLEDISIVENGVFNFVKGESF
                     TDLNNDLITDIEDFAIADRNVFKIVNLISPASH"
     gene            complement(4098..7616)
                     /locus_tag="PLX80_09500"
     CDS             complement(4098..7616)
                     /locus_tag="PLX80_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21063.1"
                     /translation="MKKLFLLIPAVLLCFILMTGQSNQVAEKWDNVLPTGVYDPNVYV
                     SAPLPQVFDFINPNTQTRYYNNGNEVVAVSPNVRVLPRTNSYQSEVNIVRHPLNPLIM
                     FGSSNAFNNTGTLFISEGIYVTTNGGNSWFGSDTMKLSGGSPVPNQGGDPGIAIDKNG
                     VFIQTHLGFSTSGMFGNYSTDNGATWSSNFTIATGSQDKNLAASDDAPSSPYYGRTYC
                     VWSRFTAASPPIAVSYTTNGGVSWSASTDINTSVAGHYSQGCDIRTGPNGEVYVAWAA
                     PTSGSPFTEDFAGFAKSTNGGVSWTVTNNAYDMNGIRGTFPTKSNIRVNGFTRIDVDR
                     SGGSRNGWIYIVTSEKNLAPAGTDPDVIMHRSSDGGATWSGGIRVNQDPLNNGKYQYF
                     PAIRVDEAGGVNIVYYDDRNTASDSSEVYISRSLDGGNTWIDMLVSDHRFKPKSIGVS
                     GIATGYQGDYIGMTSSNGKLWPLWMDDYSGIYNSWTAGVVITSFPLNPFNLNTPNAGA
                     RIETSPGNLTNNNFTWDTSASTATYKWIFGSPLASSRQISLTSGGNSFNVTSGTLDAL
                     LAGLGVNYGDSLVGQWDVWAYRNNITNDSLKAGNGPRSITLKRGIPATITTSADSLIA
                     YLPVGPNNTVKNLNIGNIGQFNLNWTMTESSVVLDNARTENMFSADILRQIENMPKGA
                     SDIYNGPEVTDGMGGPDAGGYSWIDSDEPGGPVYNWVDISGVGTQITTWTNGTGDDGS
                     ANVALPFSFSYYGNNYSNLKICTNGWVSFDAASTNTAYSNVALPTAAEPNNILCPFWD
                     DLDVRTSGQIFYYNDAVNNRFIVEYKAVPHYSSGELYTFEVIIYSDGRIYYQYQSIGA
                     LNNSNTIGIENSTGTIASQMVFNNVYVHNNMAIKLEKGIAWVDESPTAGTVNPLSSQN
                     VNVTFNSTGLSAGTYAGVLKVNSNDPVTPLKNVNVKLIVGQTAPAQITLINEGYLNLS
                     NKLNIKDTVRVYLRNTSPPYAIVDSAKGTIDSVNYTGSFLFANASNGTYYIQVKSRNC
                     IETWSKPGGEAYNSGFSLVYNFTTASSQAYGSNLKLINSKHCIYSGDVTQDGLIDLSD
                     LQNVDNSAFNFAIGYVTDDLTGDYVVDISDLTITDNNAFNFVTKNVPPGAGPVLKNPV
                     IRENASKQTILDNSKYYKNMLPGFSK"
     gene            complement(7900..10767)
                     /locus_tag="PLX80_09505"
     CDS             complement(7900..10767)
                     /locus_tag="PLX80_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21064.1"
                     /translation="MKTKLLVLGVIVTVVWIFNSGFSTNDEDPKWSNHPSTRMDINPV
                     GKYTALPEGDNRNFSNETRYISTPQGVFAVGPNFRVVPSLITQSETPITRHPTNSKIM
                     FASANTYNNGVGAGFSTGHYVTTDGGVSWFGDDVTVTSFGDPAPMVMHTNRLIISFIT
                     TTGSMGSSYSTDLGTTWSGTYTFPGASNSADKNLSAVDGIPTSAYYGRLYTVYTEFAG
                     AYAGRIVATYSNNGGVTWSAVQPVSPVPSAGHHHQGCDVTVDVFGWVRVVYANCTTNG
                     QNSTEDYLGWAESGDGGVTWGDMSDTKVNTNGIRSADLLPSPPAGVIRMNGFPRIDGD
                     KTCLSTAGDEYVVMAEKNFAPALDNADIVLNKTGDGGSSWTRVRVNQNASGSYEYSPA
                     VNVDATGAINVCYYSTRNSPANDSAEIYLSRSTDGGVTFTDVKISDHKFKPLPIPGTA
                     GGYQGDYIGITSGGNGKILPYWCEQNTSTGGRYQAYTALVDITQPRPCEDFSCALDTG
                     TATTGVITQNFYEEYSGSNFWNRRSAVSAYGSGTGSARFNSWSASVGTTQSLTSYNFS
                     PIVIGNDVYFTFDEAYRPWTGGNVDSLIVESSVNSGSTWSVRARLWGGLGAQAGPLNT
                     VFIGGGQFTPSSREWRSKIYKLPIFTNKVRIRAVSGFGNDIWVDNLCVQTLPDAVPNS
                     IGLLNQGMFIPNFPYWTFPDTVDVHLFRVDFPNIAVDSATSVVANNAIVDDLFFNKAF
                     SGNYYRVVRHRNALETWSNTGVSYTRGAASHHNFVPDGASYGNNMAIVSTTPFYRAQY
                     SGDCNQDDFIDIADLTIIDNSAFNFGSGYIVADLNGDFFADISDYAIADNNANNFVGI
                     EKPPGAEPLPPTEDINTVPVLETEQDRIKWELHKQVMSQKGNTFEVENKVMPELNDVL
                     KERDLRAKDNKAPLRKIENTQYNTKDEGNTNSNNGKRVGEK"
BASE COUNT         3485 a         1966 c         1840 g         3639 t
ORIGIN      
        1 ttactaaaaa aattataaaa gaactaaaag aagaagaaaa taccttaaga gataataaaa
       61 aaaagtataa cccggttaca aatcccgaag cattatttaa cattcttgtc tcaaagttaa
      121 cagattattc taaagaacat ttttatgtga tctctttaga ttcaagaaat atttttattg
      181 gtctggatga gatttctgtg ggtactctta ctgccagcct tgtgcatccc cgtgaaactt
      241 ttgaatcagc tataaaacgt catgcagctc atataattat agctcataat catccatccg
      301 gtgaaaccag cccatctgaa gatgatctga aaattaccag aaggctgata gatgcaggaa
      361 agataatggg aattacagtt ctggatcata taatatttgc taaacattct tatatgagct
      421 ttaaagaaag ttcattgata taaaaagaaa atagtttagc tgtaaaaact ataactcttc
      481 accatctgca aattcttcat ttacatcatc aaacaaaatc attgcctgat gaaaattacc
      541 gtcctgagaa attgcgacac caccttcagt ttcccatccc tgattaagca tttcattgac
      601 tttcttgaca agttcactat ttgaatcagc agttacgatt gtatattcca ttttttttta
      661 ttttatttat tttaaaattg agtggatttt ttagaaattg cattggtaaa ttagacaata
      721 tttgagataa ttaaaacgcc tattttgatt tgttttatgg acttaaatta atgcttgggt
      781 acatcagata tatcataact ttcccgttcg tattctggct actgtttgta aaaattattg
      841 aaactgaaac tttgtttcaa acactatttt tgtaatttat aaacagcagt gatttcattg
      901 gttctatata aactgtctgc ataagtttta taaaagagat tctcctcaaa agtaaatact
      961 gtgatttcaa aattttatta taatgttgct tggacagatg tgaaatattt ttaaattata
     1021 actactttag ttttcattga taaataaatc tattaaatct attttagttt taaaaaattt
     1081 atgacacagg cagaaatgca aactgaattc agcagattag ttaacagagg aattatattt
     1141 ggatctatat ggatttttgg aattggttct ataatcgctt ttacttcagg ctttcaggct
     1201 attaaattat ataagcaatc agacaaatct ctttcaggaa aaaataaaat ctttaaatgt
     1261 tttctcattg gagctattgg tttgctttta gatttaactg cagttctgat cataattctt
     1321 ttcagaaaaa aataattaat taagtaaatg gaatacaaag attattataa tgttttaggt
     1381 gtaaaaaaaa ctgcaacaca ggatgaaatt aaaaaatcat acagaaaact tgcattaaaa
     1441 tatcatcccg ataaaaacaa agataataag gaagctgaag aaaaattcaa agagatcaat
     1501 gaagccaacg aagttttaag tgataaggat aaaagaaaaa aatatgatga acttggtgag
     1561 aactggaaat accatcaaca gtcaggcggt gctcaggatc agggatttga ttggtcacaa
     1621 tacgcaaatc agggcagcgg atacacacaa tattcttacg gacaagatca aggagattca
     1681 tttggcggat caaatttttc ggattttttt gaaacattat ttggaggtgg cggcggattc
     1741 agttcatcag gcagaggcgg gagatcacgt gcagcaaggg caaatatgcg tggagaagat
     1801 ctgagtgccg aaatgagtat ttctctggaa gatgcttaca aaggtaccga aaaaatgttt
     1861 gaattaagcg gtcagtcgat caaactaaaa ataaaaccag gcattgcaag cggtcagact
     1921 ttaagactaa gtgggaaagg tggcaaggga gtaagcggtg gaagttccgg agatttactg
     1981 ctgacaattc atgtgcttca ggatcctgta tatgaaagga aaagtgatga tctttatacc
     2041 gatgtaaaag ttgatctcta taccgcagtg cttggtggca aagctacttt aaaaactttt
     2101 aaaggaaata ttaatttaaa tatacccaag gaagctcaga gcggaaaagt tttaagacta
     2161 caaggacttg gaatgccaaa atacggtaag gtaaatgaat tcggtgatat gtacgcaaag
     2221 attattgttg aaactcctgt gaatctgaat gagagagaat taaaactgtt tcaagaactt
     2281 tctgatatga ggaaataaac tctttatctg ttatccgaga atttttgatt caataattat
     2341 ctaatttttc taaaatctca atgtgatgca ggagaaatta gatttacaat tttaaaaaca
     2401 ttcctgtctg cgattgcaaa atcctcaata tcagtaatca gatcattatt cagatcagta
     2461 aatgattctc ctttaacaaa attaaataca ccgttttcta ctatactaat atcttcaaga
     2521 tctacaagtc cgtcattatt cagatctcca ctaaaaatac aatttaagtc accaactgaa
     2581 gtctgattgt ttccaaatgc ctgagatgaa tgacttgaaa aatcataaag atttaaattt
     2641 tgtgaaataa gaaaagaccc gcctgatctg ctccacgttt caagatattt accattatca
     2701 agtactatat aataatttcc gctgactgca ttcataaaat agagttcagt attgaaatta
     2761 tggtttttta taaatgcttg agaagaatct ataatttcat atggactgga agaatttctg
     2821 agataaactt ttaatttaaa cggatagaaa ttaaatttat taattaaaga ttcaggaatt
     2881 acttttagac tgagggtaat ggatggagta cctgtagtaa atttccatga ttcattataa
     2941 gctccctgag gcggaatacc accctgcttc actctccagt aataatcagt ttcaaattca
     3001 agatcagaag taaaaaattt taccggatct attccgtctg gtctctcaat atcttcgtca
     3061 attaaaatat ctgaaaataa agaatccgtt gctacctgaa aatttgcaga ataatttaca
     3121 gggaatcctt tttgccactc aaattttaca ggccagaaat tatttattga attgtttaaa
     3181 ggaaatattc tgaccggtgg tgtaagaggt acactcaagc agcagaactg atcatcgcaa
     3241 agatcatttg tactgaaatt atttaaggaa ttttgcaagc tcacaatact gccagaaaca
     3301 ttagctttaa tttctgttgt caaaccagaa ttatttagtt tccaaacata ttgttcctca
     3361 aacaaacatg aatttaaata tagatcagga ttgaaaaatc tgatacgggc tatcagtgtt
     3421 ccctcctcat gagagattat tggttctgta ccctgagtaa taatattgcc ttcatttata
     3481 aaaagagtat ccccggatct gataagatca cctacattat ttggagatat ccttcttctt
     3541 ccaaaagtcg atgtgtcttt agaataataa taatttaaat gatccggtag aaatgattga
     3601 tccagaataa ttgcaaattt acctgatgaa tatctgaatt cagtctgaag gaaatttgta
     3661 tgttttaata caacatcaaa atatccaaga ttagaagaaa gataatttct gttagttagc
     3721 tttaactcaa aattaggatt ttgagaataa gaaattgacg taataataca aagtaagaat
     3781 atagaaaaaa ctaattttct aaaattcata atttagaata atttaatctt aaattaatat
     3841 ttgcatacaa tatacttcaa tcagaattta ttaaaaaaat tgaatgaata ttatttatcc
     3901 gaaatatcga aaattgcaaa taacattaca ggttaaagag atcaagtttt ggggttgata
     3961 ctttggcctt tggaaagcaa gatatatatt cttattttaa tttaaaactt aatattttca
     4021 atacaaaaat aagtttatga aaataaaaaa agccgtaatc tgcattacgg ctttttagtt
     4081 taaatcttaa tttaacttta ctttgaaaat ccggggagca tatttttata gtattttgaa
     4141 ttgtcaagta ttgtttgttt ggaggcattt tctctgataa caggattttt taacacaggt
     4201 cctgcacctg gaggtacgtt cttggttaca aaattaaatg cattattatc agtgattgtc
     4261 agatctgaaa tgtctacaac atagtcacct gttagatcat cagttacata accgattgca
     4321 aaattgaatg cactgttatc aacattctgc agatcagaaa gatcaatcaa tccatcctgt
     4381 gtaacatcac cactatatat gcaatgtttg gaatttataa gcttaagatt tgaaccgtaa
     4441 gcctgagatg atgcagttgt gaaattatat accaatgaga atccggagtt atatgcttca
     4501 cctcccggct tactccacgt ctcaatgcag tttctgcttt ttacctgaat ataatatgtt
     4561 ccattcgagg catttgcaaa taaaaatgat ccagtataat ttacagaatc tatagtacct
     4621 tttgctgaat ctactatcgc ataaggcggt gaagtatttc tcagatatac tcttactgta
     4681 tctttaatat ttaatttatt tgacaaattt aaatatcctt cattaataag tgtgatctgc
     4741 gcaggagctg tctgccctac aatcaatttt acatttacat tttttaaagg tgttacagga
     4801 tcattggaat ttacttttaa aactcctgca tatgtacctg cagaaagtcc tgtactgtta
     4861 aacgttacat tcacattttg agaagacagt ggattaacag tacctgcggt tggagattca
     4921 tctacccatg caattccttt ttcaagttta attgccatgt tattgtgtac ataaacatta
     4981 ttgaaaacca tttgtgaagc tatggtacct gttgaatttt caataccgat cgtattagaa
     5041 ttattcagtg ctccaatact ctgatactga taatagattc tgccgtctga ataaataatt
     5101 acttcaaatg tataaagttc tccggaacta taatgaggca cagctttata ttctacaata
     5161 aatctgttat tgactgcgtc attataataa aatatttgac ctgatgttct gacatcaagg
     5221 tcatcccaga aaggacaaag tatattattt ggttctgcag cggtaggtaa tgctacattt
     5281 gaataagctg tatttgtgga agctgcatca aagcttaccc agccgtttgt acatatcttt
     5341 aaattggaat aattatttcc ataataactg aaactaaaag gcagagctac attagctgag
     5401 ccgtcatcac cggttccgtt tgtccaggta gtgatctgcg ttccgacacc ggaaatatca
     5461 acccagttat aaaccggacc gcccggttca tcactgtcga tccatgaata tccgcctgcg
     5521 tcaggtccgc ccattccgtc cgttacttcc ggtccgttat aaatgtcaga agcaccttta
     5581 ggcatatttt ctatctgtct taatatatca gcactgaaca tattttctgt ccttgcattg
     5641 tctaaaacta ctgaagattc tgtcatagtc caattaagat tgaactgacc tatatttcca
     5701 atatttagat ttttcactgt attattcgga cctaccggaa gatatgcaat cagtgaatct
     5761 gccgaagttg taatcgtagc gggtatacct ctttttaaag taattgatct cggaccatta
     5821 cctgctttca aagaatcatt tgtaatgtta tttctgtaag cccatacatc ccattgtcct
     5881 acaagtgaat caccataatt tacaccaagt ccggcaagta aagcatctag tgtaccgctg
     5941 gtgacattaa atgaatttcc tcctgatgtt aatgaaattt gtcttgaaga agcaagagga
     6001 ctgccaaata tccatttata tgtagcagtt gaagctgaag tatcccaggt aaaattattg
     6061 ttggttaaat ttccgggaga tgtttcaatt cttgctccgg cgtttggtgt gttcagattg
     6121 aatggattta aagggaatga agtaataact actcccgcag tccacgaatt ataaattccg
     6181 gaataatcat ccatccataa cggccatagt tttccgttac tggaagtcat gccaatgtaa
     6241 tctccctgat atcctgttgc aatacctgag acaccaattg attttggctt gaatctatgg
     6301 tcactgacga gcatatcaat ccaggtatta cctccgtcaa gagatcttga gatataaact
     6361 tctgatgagt cagatgctgt attcctgtca tcataatata caatatttac accgcctgct
     6421 tcatccactc tgatagccgg aaagtactgg tattttccat tattaagcgg gtcctgatta
     6481 actcttattc cgccggacca tgtagctccg ccgtctgaag atctgtgcat tataacatca
     6541 ggatcagtac ctgcaggtgc taaatttttc tctgatgtaa caatgtagat ccatccgttc
     6601 ctggaaccgc cgcttctatc gacgtcaatt ctcgtgaatc cattaactct tatgttggat
     6661 ttagtcggga atgtacctct tattccgttc atgtcatatg cattatttgt aactgtccag
     6721 cttacaccgc cgtttgttga ttttgcaaaa cctgcaaagt cttcagtaaa cggacttccg
     6781 gacgtcggag ccgcccaggc tacataaact tcaccatttg gtccggttct tatatcacaa
     6841 ccttgtgaat aatgtcctgc aacagaagta tttatatcag tagatgcaga ccagcttaca
     6901 ccgccgtttg ttgtataaga aaccgctatc ggtggtgagg ctgcagtaaa tctggaccat
     6961 acacagtatg ttcttccgta ataaggactt gatggtgcat cgtctgaagc ggcaagattt
     7021 ttatcctgcg aacctgtagc aatagtaaaa ttgctcgacc atgttgcacc gttatctgtt
     7081 gaataatttc caaacatacc tgaggttgaa aatccaagat gagtttgaat aaaaacaccg
     7141 ttcttatcaa ttgctattcc cggatcacct ccctgatttg ggacaggtga accgcctgat
     7201 aatttcattg tgtcagatcc aaaccatgag tttccaccgt ttgtcgtgac ataaattcct
     7261 tcactgataa ataatgtacc tgtattatta aaagcatttg aagaaccaaa cattatcaat
     7321 ggattcaacg gatgtctgac tatgttaact tcagactgat agctgtttgt tcttggcagc
     7381 actcttacgt ttggtgatac tgctacaact tcatttccat tattataata tctcgtttgt
     7441 gtatttggat ttataaaatc aaatacctgt ggcaatggtg ctgaaacata tacattggga
     7501 tcgtaaactc ctgttggaag tacgttatcc catttttctg caacctgatt actctgtcct
     7561 gtcattaaaa taaagcataa caatacagca ggaattaata agaataattt tttcaattaa
     7621 gatttccctt tctgaatttt tttaattttt tgattaattc tgttaattag atttgaataa
     7681 atatataggt gtttttgaaa tttttaaaat cagaaatgga aaatcatttg tcaatagttt
     7741 tgaattttat agacagaact atgcatgatg gaattgtata taaagtaaat taaatatttc
     7801 gaactttcaa ctatctttta taatccgtaa gcaaataaaa aaagccgtga agaaaattct
     7861 ccacggcttt ttgttttatt ttaattactg accggttgat tatttctcac cgaccctttt
     7921 accattatta ctgtttgtgt tgccttcatc ctttgtatta tattgtgtat tttctatttt
     7981 tctcaaagga gctttattat cttttgctct cagatctctt tcttttaaaa catcattaag
     8041 ctctggcatt actttatttt caacttcaaa tgtattgcct ttttgactca ttacctgttt
     8101 atgcaattcc catttaattc tgtcctgttc tgtttcaagt acaggtactg tatttatatc
     8161 ttctgtaggc ggaagtggtt ctgctcccgg tggtttttca attcctacaa aattatttgc
     8221 attattatcg gcgatagcat aatcagaaat atctgcaaag aaatctccgt tcagatcagc
     8281 tacaatataa cccgacccaa aattaaatgc agaattatca attattgtaa gatctgcaat
     8341 gtcaataaaa tcatcctgat tacagtctcc actgtactga gcccggtaga atggtgtagt
     8401 actaactatt gccatattat taccataaga tgcaccatca ggaacaaagt tatgatggga
     8461 tgcagcgcct cttgtatatg atactcctgt attactccaa gtctccagag catttctgtg
     8521 ccttactact ctgtaataat ttccactaaa agctttgtta aaaaacagat catcaacaat
     8581 tgcattattt gcaactactg aagttgcaga atctactgct atgtttggga aatcgactcg
     8641 gaataaatga acatctactg tatcagggaa agtccagtaa ggaaagttcg gaataaacat
     8701 accctgattt aaaagtccta tcgaatttgg tacagcatca ggtaatgtct gtacacatag
     8761 attatctacc caaatatcat ttccaaatcc gcttactgct cttattctta ctttatttgt
     8821 aaatattgga agcttataaa tttttgatct ccactctctg gatgatggtg taaactgacc
     8881 gccgcctata aatactgtat ttaaaggacc ggcctgagca cccagtccgc cccataatct
     8941 tgctcttact gaccatgttg aaccgctgtt aactgatgac tctactataa gtgaatccac
     9001 atttcctccg gtccatggtc tgtatgcttc atcaaatgtg aaatagacat catttccaat
     9061 tacaatcgga gaaaaattat atgaggtcag tgattgagta gtgccaacgg aagcagacca
     9121 tgaattgaat cttgcagatc ctgttccaga tccgtatgca ctgacagctg atcttctgtt
     9181 ccagaaattt gaacccgaat attcttcata aaaattttgt gtaattactc ctgtagtagc
     9241 tgtaccggta tctaatgcac atgaaaaatc ttcacatggt cttggctgag taatatctac
     9301 caaagctgta taagcctgat atcttccgcc ggtagaagta ttttgttcgc accagtatgg
     9361 taagattttt ccatttccac cggaagtaat tccgatataa tcaccctgat atccgccggc
     9421 agttccggga attggcagtg gcttaaattt atgatcagag atctttacat ctgtaaatgt
     9481 aactccgccg tcagttgatc ttgaaagata aatttcagca gaatcatttg caggagaatt
     9541 tcttgtactg taatagcaga cattaattgc tccagtagca tctacattca cagcaggact
     9601 atactcatat gaccctgatg cattttggtt tactctgact cttgtccatg aacttcctcc
     9661 gtcaccagtt ttatttaaaa ctatatcagc attatcaagt gcaggtgcaa aattcttttc
     9721 tgccatgact acatattcat caccggctgt tgatagacag gttttatccc cgtctattcg
     9781 cgggaatcca ttcatcctta ttactcctgc aggtggtgat ggtaaaagat ctgcggatct
     9841 gataccattt gtatttactt tagtatccga catatcaccc caggtaaccc caccgtctcc
     9901 tgattctgcc catcctaaat aatcttcagt agagttttgt ccgtttgtag tacagtttgc
     9961 ataaactact cttacccatc caaaaacatc tacagtcaca tcacatccct gatgatgatg
    10021 acctgcagag ggaacgggac tgaccggctg aacagctgac catgtgactc cgccgttatt
    10081 tgaataagta gctacaattc tgcctgcgta tgctcccgca aattcagtgt aaactgtata
    10141 caacctgccg tagtatgcac tggttggaat tccgtctact gcagaaaggt ttttgtcggc
    10201 agaatttgaa gcacctggaa aagtataagt tcctgaccat gtagtaccta aatccgtaga
    10261 atatgaagat cccattgatc cggttgttgt aataaaggaa atgatcagcc tgttagtatg
    10321 catgaccatt ggtgccggat ctccgaaact tgtaacagta acatcatcac caaaccagga
    10381 tacaccaccg tcagttgtta cataatgacc tgtgctgaaa cctgctccca caccattatt
    10441 atatgtattt gcagatgcaa acataatttt tgaattggtt ggatgacggg tgataggtgt
    10501 ttcgctctgc gtgattaatg aaggtacaac tctgaaattt ggtcccacag caaaaactcc
    10561 ttgtggtgta gatatgtatc ttgtttcatt actaaaattt ctgttatcgc cttccggtaa
    10621 tgctgtatat tttcctaccg gattaatgtc cattctggta ctgggatgat tactccattt
    10681 cggatcttca tcatttgtag aaaaacctga gttgaaaatc cagacaactg taacaatgac
    10741 gcctaaaaca agtaattttg ttttcatttg ggtagtcctt tttaattagt ttatgaattt
    10801 tttttttgtt ggtgattata tttatttatt ttaaatttaa ttatcaagtc catttttttt
    10861 gtaaatgaaa aaatttactg tttcattttt actcctacct tgttaaattt aatttaacaa
    10921 attgtaaaaa
//
LOCUS       DAORVQ010000109        10672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3194_length_10672_cov_6.080154, whole genome shotgun sequence.
ACCESSION   DAORVQ010000109 DAORVQ010000000
VERSION     DAORVQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_10672_cov_6.080154"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLX80_09510"
     CDS             complement(<1..693)
                     /locus_tag="PLX80_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HQY21065.1"
                     /translation="MLNDLTNKLETVLKKIRGQGKITDSNIDESLREIRRVLFDADVN
                     YKVVTQFINDVKEKSIGLNVLNSVTPGQLIVKVINDELIKLMGSERKGIKFSDRIPSV
                     ILLVGLQGSGKTTFSAKLAKLLKTQGRNTLLAACDVYRPAAIEQLKVLGKQIGIPVYT
                     DENEKDPVKIANAAVQYAKENARDTLIVDTAGRLAIDEEMMNEVERLKKSLDPEEILF
                     VVDAMTGQDAVNT"
     gene            863..1432
                     /locus_tag="PLX80_09515"
     CDS             863..1432
                     /locus_tag="PLX80_09515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQY21066.1"
                     /translation="MIICGIDEAGRGPLAGPVVAASVLFEEGFFIEGVNDSKMLTPAQ
                     RENLFPVIIESCIEYSISEIDNNVIDEINILKATMLAMYNCISESKITPDKYFIDGNY
                     FRLPDDHHTKINFETVIKGDSKIFAISCASILAKVHRDILMREFHFIHAEYDFHNNKG
                     YGTKKHIEKVKELGLSSIHRRTFCKNYFV"
     gene            1439..1819
                     /locus_tag="PLX80_09520"
     CDS             1439..1819
                     /locus_tag="PLX80_09520"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQY21067.1"
                     /translation="MADELKCVYKNRFGKNGEMIARNFLTDKGMKFLKANYRYDRAEI
                     DLIFEDLKTGTVIFAEVKTRKNKKFGEPEESVNPAKQERIKKAAMGFILENESYSSHD
                     LRIDVISILFTEGVYEINHIENAF"
     gene            complement(2663..3529)
                     /locus_tag="PLX80_09525"
     CDS             complement(2663..3529)
                     /locus_tag="PLX80_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21068.1"
                     /translation="MRILILILAVSLFLLKDVHAQSFAGKDDIKFWQWYNSKPSVEVT
                     YGNSNISFGNTGYNFAPSGLIEMNLGFTSKNISDFGKNIVRYKNRFIFLGYSSTDNST
                     RQTSSGINSNLWKFGFGNKSGYGVSLSKTASILPYSSSTFNWSNFNYNKPNDINSNIS
                     EQEYYAAIDNMAGVFRFGSSFEAGINLNVIKGFSIQPKYEIADIFPRHLVGYQMMSSA
                     IELTGFTMLEMFTKKIIRNSPVAGTFVNFLLLNAYQYGFYQLKKDQMFWPFTSTAPLR
                     YETFKLGMTFVF"
     gene            complement(3759..5615)
                     /locus_tag="PLX80_09530"
     CDS             complement(3759..5615)
                     /locus_tag="PLX80_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21069.1"
                     /translation="MKKFTFSFLLLSALILNLVSNSYAGDRKVVIERFTSSTCGPCAS
                     QNPNLDAYLGSSDPNKVLSLSYHMSWPAPGNDPMYLINIADNNSRRSNYGVNAIPTWV
                     FDGLTSITGGSTSTVSNLVNARTDVLSPVTIIMSETRTGTTVNTTVKIYCEKVLPNPN
                     ATVHFVISEKFVQYSFPPGTNGETSFHDVMRKMLPNGIGTAVTLLPGKVITLNYTYQL
                     DPSWDADQIESVVMVQGAPLEIHNTGTLTHDFNLYSAPAYRVVNQGVTQDANYKVHIP
                     SVADGYNSAVTFTSEVIPATAGITVNFSNGNVISNFPDSLDVNVSSNGSVPAGEYTVV
                     LTGTSASGKVHKTIINYLVGRSYIVTGTDRGTLAYKVDGTSYSTPRVFTWDLNSQHTL
                     EAVSPQTVGNFKYLYDSWTNGGSQTQTVTINTTDVAYAAIYDVQYRLLGLLNPAGLPV
                     TISGGVGSYIDSGAVVNLNLSALQVSHNGRTYYFNRWEGQGEGSYTGPNTSAQFNMNS
                     VIVQTAVYDTIDVGISNYSSLVPDKFSLYQNYPNPFNPSTNIRFDIAKSTFASIVVYD
                     ILGKEVSQLVNQVLTPGSYQYSFNAGNFPSGIYYYKIQTDQFTDIKKMMLLK"
     gene            complement(5680..7782)
                     /locus_tag="PLX80_09535"
     CDS             complement(5680..7782)
                     /locus_tag="PLX80_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY21070.1"
                     /translation="MASQIKYLQNIILIFILFSTSYRDSVSQPFVQEFDGINFSLPAG
                     NSYTPFNGGQNNARIQFVDIDGDNDLDLFTYDADTSLYFYRNTGNSQTPQYKFVSGRF
                     QDLRFYNWFYFTDIDNDNDVDLFAGGDLQSVMFFRNTGNVTTPAFNLEIQELRSSGDT
                     VIYSEANCVPIFKDLDNDGDKDFFTGQSLGTITFYENTGTSVNFEFSFRTDLWQNLLI
                     ISPAFDNKDNRHGANALEFTDIDNDNDFDLYWGDLFSKGIYFIRNDGTPEAANVVIAD
                     SIYPRNSPYESLGYNSTRFTDIDNDGDQDMFVSVLYLSQNKNNFTYYRNDGSAGNPLF
                     NRLTDNFLNNVDVGGSSNPAFADLDNDGDKDLLMGNDYSTLTYYKNTGSISDPSFTLI
                     TDSLPVLSESFNYSPCLSDLDNDGDKDLILGSYLRDSLWFFRNTGTAENFVFTLESRG
                     HQMGLTTLGQSTTPALVDIDGDNDLDFFSGGTNGRLIYYENTGSASAFNFTFISNFYS
                     NIDAGDDSAPRFFDIDNDGDFDLFIGKLNGQISFYKNNGTPQSPLFEIENVNYGGINV
                     HQNAVPYFIDINNDTDLDLFIGNIKGGLYYFRNDKISSVNISQTELPSSFRLEQNFPN
                     PFNPSTVIRFESRLNTDIRLSIFDINGKEVEVLYNGAVTQGSHEFRFNAAPYSSGIYF
                     ARLQSGINIQNIKMMYLK"
     gene            complement(7766..9547)
                     /locus_tag="PLX80_09540"
     CDS             complement(7766..9547)
                     /locus_tag="PLX80_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQY21071.1"
                     /translation="MRKIFTPVILLISLMLIIFSSAETSNLRSAELIKTKQVSDNFFG
                     LDNSDNTWVANKLASMTLREKIAQMIISYSDGFLVNESSSEFKRMSNLIENEKVGGFI
                     FFKGNSVEEAKLINMLQEMSETPLLMSADFERGTKMRLNDGSLFPNNMALGATGNTDL
                     AYQMGLQIAKECRAIGIHQNYAPVLDINNNPDNPIINVRSYGEDPLLVSKLGLSFIQG
                     MQEGGVIATAKHFPGHGDTDIDSHSDLPVLNFSRERLDNLELIPFQNAVDAGVMSVMI
                     AHLSLPSIDDEAFLPASLSEKIIDDLLIRQMGFKGLVVTDALNMAGIVKHFSTEDVAL
                     RCVDAGVDLILMPQGETTTISTIENAVTNGSISEERIDRSVKKILDAKNWLKLHENKY
                     SEPSEVLNIVNSSSAKKISQDIADESITLVKNEGSILPFQNISDKTCLIISLNNGNEN
                     ANSNLFASQFEANNKFKESYFYDLSGEISNKDELIFEAENSDIILVPIYAKVKINTGT
                     VGLPDSQISLINSLIATGKKVIVISFGNPYLIKGFPDVDSYVCAYADAESTIISAINA
                     LNGIIKFKGKLPVSISQEFKLGHGITN"
     gene            complement(9582..10379)
                     /locus_tag="PLX80_09545"
     CDS             complement(9582..10379)
                     /locus_tag="PLX80_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQY21072.1"
                     /translation="MEALAGVLFVFAGILGFILFIAVLRAFRVLNEYERGVIFRLGKF
                     SAVKGPGLIILIPIMDKMVKMSLRTIALDVPSQDIITKDNVSVKVNAVVYFRVVDPNR
                     AIIQVEDFMYATSLISQTTLRTVTGQGDLDDLLTHRDLVNSKLRELIDKETEPWGIQV
                     LNVELKNVDLPQEMIRAMSRQAEAERDKRAKIINSEGEFMSAQKLGEAADILGKSQNA
                     LQLRYLQVMTEIAAEKNSTMIIPLPMEIMRYFTAMTDSLNNAQNKKD"
BASE COUNT         3352 a         1999 c         1814 g         3507 t
ORIGIN      
        1 tgtattaact gcatcctgtc cggtcatcgc atccacaaca aaaagtatct cttcaggatc
       61 aagcgatttt ttcagtctct cgacctcgtt catcatttct tcatctatag caagacgtcc
      121 tgcagtatcc actattaaag tatcacgcgc attttctttt gcatactgaa ctgccgcgtt
      181 tgctatcttt acaggatctt tttcattttc gtctgtataa acgggaatgc cgatctgttt
      241 gccgagtact ttgagctgct ctattgccgc cggacgatat acgtcacatg cggcaagcag
      301 cgtatttctt ccctgggttt taagcagctt tgccagcttt gccgaaaaag tagtctttcc
      361 cgacccctgc aaacctacga gtaaaatcac cgaaggaatc ctgtcagaaa atttaattcc
      421 tttcctctca gatcccatca gctttatcag ctcatcatta attactttta caatgagctg
      481 accgggagta acgctgttca atacgttaag cccgatggat ttttctttta catcatttat
      541 aaattgggtt acaactttat aattaacgtc agcgtcgaac aaaactctcc tgatctctct
      601 cagagattcg tctatgttgc tgtcagtaat ttttccctga ccccggatct tcttaagaac
      661 ggtctcaagt ttgtttgtta aatcgtttaa cattaagatt atttgtagct gtaatattcc
      721 aaattagtgg gctaaaaata ccaatattaa taaagtttat caaggtatat aatttcatta
      781 gtctgtttct attttttgta aattaagata ttttattcgg aaaatcctga ataattaatt
      841 tattttatta aattttttaa aaatgataat ttgcggaata gatgaagcag gccgaggacc
      901 acttgccgga ccggtagtgg cggcatctgt tttgtttgaa gaaggatttt ttattgaagg
      961 cgttaatgat tctaaaatgc tcacacctgc acagagagaa aatctctttc ctgttattat
     1021 tgaaagctgt atcgaatatt caatcagcga aattgacaat aatgtgatcg atgaaattaa
     1081 tattctcaaa gctactatgc ttgcaatgta taactgcatc tctgaatcta agatcactcc
     1141 tgataaatat tttattgacg gaaattattt ccggctgccg gatgatcatc atacaaaaat
     1201 aaattttgaa actgtcataa aaggagattc aaaaatattt gcaatatcat gtgcatcaat
     1261 tttagcaaaa gttcacagag atattctcat gagagaattt catttcatac atgccgaata
     1321 tgattttcat aacaataaag gttatggcac aaaaaagcat attgagaaag taaaagaatt
     1381 aggactcagc agtattcacc gcaggacttt ctgcaaaaat tattttgtct gaattaatat
     1441 ggctgatgaa ttgaaatgcg tttataaaaa cagatttgga aaaaatggtg aaatgatcgc
     1501 tcggaatttt ttgacagata aaggaatgaa atttctgaaa gcaaattacc ggtatgatcg
     1561 cgcagaaatt gatctgatat ttgaagactt aaaaaccggt acagttattt tcgctgaagt
     1621 gaaaacaagg aagaataaaa agttcggcga acctgaagaa tccgtaaatc ccgctaagca
     1681 ggaaagaata aaaaaagccg caatgggatt catacttgaa aatgaatctt actcatcaca
     1741 tgatcttcgt attgatgtga taagtatttt atttactgaa ggcgtatatg agataaatca
     1801 tatagagaat gcgttctgac aagaagtctt ttttaccact aagactcgaa gactcgaagg
     1861 ctcgaaggct caaatatgag atgattaaaa tgtttgaaaa aataatttat ttgatccaat
     1921 aattcataag gttaagcaat tcctaaaaaa tgaaaatcat tcctaattcc taattcgtaa
     1981 ttcttaattc taaattgaaa agtcattccc gctcttactg tcattccctc gaaagcggga
     2041 atctccaaat gaaacattta tccaaataat ttagcaatca ttttttactc ttcaataatt
     2101 taaaaaaact gaatatcagc aattcataga ttccggtttt cgtgtgtagt tcagagattc
     2161 ccgcttgcgc gggaatgata gtgtgggtga gttccaccgt aggtttatcc ggcgattttc
     2221 cgggactccg actaaatgta cagcatcggc agagtccacc aaaaattgga gaaaatagcg
     2281 taaaatattt tgaaaaaggc agtgtccgct gcttcaagcc aattgtccgc cgctcgaagc
     2341 caattgtccg ccgctccaag ccaattgtcc gctctcaatt gcgaagtatt attctttaaa
     2401 tgaaattttt tatattcttt acttggaaag gaatgtttct gaaaactaaa taaattgtat
     2461 tacaacagag acacaaagac acaaaattca tattttaaac ttttttcttt tgtatctcct
     2521 gtataagtca tttacaaaat gacgctatca ctgctaagtc accggagtcc gaagagcacg
     2581 tgactccgtc ggaacggttt aactattttt ctttgtgtct ttgtgtcttg gtgccttggt
     2641 ggtcaaagcc ggtagttgtt tatcaaaaaa caaaagtcat cccaagctta aaagtctcat
     2701 atctcagagg tgctgtgctt gtaaacggcc agaacatctg atctttttta agctgataaa
     2761 atccgtactg gtatgcatta agtaaaagga agtttacgaa agtacctgct acaggagaat
     2821 ttctgattat cttttttgta aacatttcga gcatagtaaa tccggttaat tcaatagctg
     2881 aactcatcat ctgatagccg actaaatgtc tggggaatat atctgcgatc tcatatttag
     2941 gctgaataga aaatcctttt ataacattta aatttattcc tgcctcaaat gaacttccaa
     3001 acctgaatac acctgccata ttatcaattg ctgcatagta ttcctgttca cttatatttg
     3061 aattaatgtc atttggttta ttgtaattaa aatttgacca gttaaaagtc gaagaagaat
     3121 agggtagtat tgaagctgtc ttactaaggg aaactccgta cccgctttta ttgccgaatc
     3181 caaacttcca gagatttgaa tttatacctg atgaagtctg ccttgtagaa ttatccgttg
     3241 aactataacc caaaaagata aatctgtttt tatacctgac tatgtttttt ccgaagtccg
     3301 atatattttt ggatgtaaat ccgagattca tttctattaa tccggatgga gcaaagttat
     3361 atcccgtatt accgaaactg atatttgaat taccgtaagt tacttccaca gatggtttgc
     3421 tgttatacca ctgccagaat ttaatgtcat cttttcctgc aaatgactga gcgtgaacat
     3481 cctttaataa gaaaagtgaa actgctaaaa taagaataag tattctcata ataatttgat
     3541 ttaaataatt aaaaaatgaa ttcatatatt tcttaagact gtaatacata acaccgggtt
     3601 tcaattgaaa tcatttccat gaacaattat tttttcaaaa caaatttata ttgaaaatgt
     3661 atctgacaac gggttaaaaa aaaacttccg aaatctaaat gatcccggaa gtttaaaata
     3721 ttatttaata tttaatattt aaaatttaat attaaatttt acttgagcag catcattttc
     3781 tttatatctg tgaactgatc agtctgtatc ttgtaataat aaattccgct tgggaaatta
     3841 cctgcattaa aagaatactg atatgatccc ggagtcagaa cctgattaac taactgtgat
     3901 acttcttttc caagtatatc ataaactact attgatgcaa atgtagattt tgcaatatca
     3961 aatctgatgt ttgtagaagg attgaacggg ttcggatagt tctgatataa tgagaattta
     4021 tccggtacca gtgaactgta atttgaaata ccaacatcta ttgtatcata aactgcagtc
     4081 tgaacaataa ccgaattcat attaaactgc gcagaagtat taggtccggt atatgaacct
     4141 tctccctgac cttcccatct gttaaaataa tatgtccttc cattatgact tacctgcaat
     4201 gctgataaat ttagatttac tactgcacct gaatctatgt aacttccaac tccgccggat
     4261 atagtaactg gcagtcctgc cggatttaaa agtccgagta atctgtattg gacatcataa
     4321 attgcagcat aagcaacatc agttgtgtta attgttacag tctgtgtttg tgatccgccg
     4381 tttgtccagc tgtcataaag atatttgaaa tttccaacag tctgaggtga aactgcttca
     4441 agggtatgct gtgaattcag atcccatgta aatactcttg gtgtagaata acttgttccg
     4501 tcaactttat aagcaagcgt tcctctgtca gttccggtca caatgtaact tcttccgacg
     4561 agataattaa tgattgtttt gtgtactttc ccacttgcac tggtaccggt aagtacaact
     4621 gtatattcac ctgcaggtac agatccatta gaagaaacat ttacatcaag tgaatcaggg
     4681 aaattactga tcacatttcc attactaaaa tttactgtga ttccggcagt ggcaggaatt
     4741 acttctgatg taaaagtaac agcagaatta taaccgtcag ctactgaagg aatatgaact
     4801 ttataatttg catcctgagt aactccctga tttacaactc tgtaagcagg tgctgaatat
     4861 aaattaaaat catgagtcag agtaccggta ttgtggatct caagaggagc accctgaacc
     4921 attaccacac tttctatctg atcagcatcc catgacggat ccagctgata tgtataattc
     4981 agtgtaatta cttttcccgg cagcagagta actgctgttc cgattccgtt tggaagcatc
     5041 tttctcataa catcatgaaa agatgtctcg ccgttagttc cgggcggaaa agaatactga
     5101 acaaattttt cagaaattac aaaatgaact gtcgcatttg gattaggtaa cactttctcg
     5161 cagtagatct ttacagttgt atttactgta gtacctgttc ttgtttctga catgataatt
     5221 gtaacaggtg acagcacgtc agttctggcg ttgaccaaat tagaaactgt cgaagtactg
     5281 ccgccggtga tacttgtaag tccgtcaaat acccaggtcg gaattgcatt tactccgtaa
     5341 ttggatcttc tggaattatt atcggcaata ttgatcagat acatcgggtc atttcccggt
     5401 gcaggccagc tcatgtgata gcttaatgat aatactttat taggatctga agaaccaaga
     5461 tatgcatcca gatttggatt ttgggatgca cagggtccgc aagtggaact tgtaaatctt
     5521 tctataacta ctttcctgtc gcctgcatat gaattactta caagattcaa tattaatgct
     5581 gaaagtaata gaaatgagaa agtaaatttt ttcattttta tatattttat tttaataaaa
     5641 agttttttga ggatttgtta aaaatagttt atttaatact tattttaaat acatcatttt
     5701 aatattctga atatttattc cgctttgtaa tcttgcgaaa taaatcccgc ttgagtaagg
     5761 agcggcatta aatctgaatt catgtgatcc ctgagtaaca gcaccattat ataatacttc
     5821 tacttccttt ccgtttatat caaaaatcga taatctgata tcagtattta atctgctttc
     5881 aaatctgatc acagtcgacg ggttaaatgg attcgggaaa ttctgttcaa gtctgaatga
     5941 agacggcagc tctgtttgag aaatattaac tgaagagatc ttgtcatttc tgaaataata
     6001 aagtcctcct tttatattac cgataaatag atcgaggtca gtatcattat ttatatcaat
     6061 aaaatacggt acagcattct gatgaacatt tataccaccg tagttaacat tctcaatttc
     6121 aaataaagga ctctgaggag ttccgttatt tttataaaat gaaatttgac cattcagttt
     6181 accaataaaa agatcaaagt caccatcatt gtcaatatca aaaaatctcg gagcactgtc
     6241 atctcctgca tctatgttgc tgtaaaaatt tgatataaaa gtaaaattaa aagctgaagc
     6301 tgagccggta ttttcataat agatcagtct tccattggta ccacctgaaa agaaatcgag
     6361 atcattatca ccatcaatat cgacaagtgc cggagttgta ctttgtccaa gagtagtaag
     6421 ccccatctga tgtccgcgtg attcaagagt aaaaacaaaa ttttcagcag taccggtatt
     6481 tctgaaaaac caaagtgaat ctctcagata acttcctaat atcaaatcct tatcaccgtc
     6541 attatcaaga tcggaaaggc agggtgaata attaaaactt tcggaaagaa cgggaagtga
     6601 atcagttatt aaagtaaaag aaggatctga aatgctgccg gtatttttat agtaagtcaa
     6661 agtggaataa tcatttccca tcaaaagatc cttatcaccg tcattatcaa gatctgcaaa
     6721 cgcaggatta ctgctgcctc cgacgtcaac attattcaaa aaattatctg tgagtctgtt
     6781 gaataatgga tttcctgcag agccgtcatt tctgtaataa gtaaaattat ttttattttg
     6841 tgacaaataa agaactgata caaacatatc ctgatcgccg tcattatcaa tatctgtaaa
     6901 tcttgttgaa ttataaccga gtgattcgta aggtgaattc cttgggtaaa tagaatcagc
     6961 aattacaaca tttgccgctt caggtgttcc gtcgtttcga ataaaataaa ttccctttga
     7021 aaaaagatcg ccccagtaaa ggtcgaaatc attatcatta tcaatatcag taaactcaag
     7081 ggcatttgca ccgtgtctgt tgtctttatt gtcaaaagca ggtgaaatga taaggagatt
     7141 ctgccagaga tccgtcctga atgaaaattc aaaattaact gacgttccgg tattttcata
     7201 aaatgtaata gttccgagtg actgtcccgt aaaaaaatct ttgtcgccgt cattatccaa
     7261 atccttaaaa attggtacac agtttgcctc gctgtagatc accgtatcac ctgaagatct
     7321 gagttcctga atttccagat taaaagcagg tgtagtgaca ttaccggtat ttctgaaaaa
     7381 cattactgac tgcaggtcac cgcctgcaaa gagatcaacg tcattatcat tatctatatc
     7441 cgtaaaataa aaccagttgt aaaatctcag atcctgaaac ctgccggaaa caaatttgta
     7501 ttgaggagtt tgtgaattac ctgtatttct ataaaaatat aatgaagtat ccgcatcata
     7561 tgtaaacaga tcaagatcat tgtcaccgtc tatgtcaacg aactgaattc tcgcattatt
     7621 ctgaccgccg ttaaatggtg tgtaggaatt tcctgcggga agactgaaat tgattccgtc
     7681 aaattcctga acaaatggtt gtgaaactga atctctgtaa gaggttgaga aaagaatgaa
     7741 gattaaaata atattctgta aatatttaat ttgtgatgcc atgtcccagt ttgaattcct
     7801 gactaataga tacaggtaat ttacctttga acttaataat tccattcaaa gcattaatag
     7861 ctgaaataat tgttgactct gcatcggcat aagcacatac ataagaatca acatccggga
     7921 aaccctttat cagatatgga ttgccaaagg aaataacgat cacctttttt ccggttgcaa
     7981 tcaaagaatt tatcagagag atctgtgaat ccggtaaacc gactgtgccg gtgtttattt
     8041 ttacctttgc ataaataggc actaaaataa tgtcagagtt ttccgcttca aatattaact
     8101 cgtccttatt tgaaatctca cctgacagat cataaaaata tgattctttg aatttattgt
     8161 tagcttcaaa ctgtgaagca aataaatttg agtttgcatt ttcatttcca ttattaagtg
     8221 aaattatgag acaggtctta tctgaaatat tctgaaaggg gagtatactg ccttcatttt
     8281 ttacaagtgt gatggattca tctgctatgt cctgagatat ttttttagcg ctgcttgaat
     8341 ttacaatgtt caaaacttca gacggttcag aatatttatt ctcatgaagc ttcagccagt
     8401 tctttgcatc aagaattttc ttaactgaac ggtctattct ttcttcagag atactgccgt
     8461 tagtgacagc attttcaata gtcgagatcg ttgtagtttc tccctgcggc atgagtatca
     8521 gatcgactcc ggcatcgacg catcttaaag ctacatcttc agtggaaaaa tgctttacga
     8581 tacctgccat attcagtgcg tctgtgacta ccagtccttt aaatcccatt tgtctgatca
     8641 gcagatcatc aattattttt tctgaaagag atgccggtaa gaaagcttca tcgtctatcg
     8701 aaggaagtga aaggtgtgcg atcataactg acattacacc tgcatccact gcattttgaa
     8761 atggaatcaa ttcaagatta tctaatctct ctcttgaaaa atttaaaacc ggcagatcac
     8821 tgtgtgaatc aatatccgta tcaccgtgtc cggggaaatg ctttgcagtc gcaatgactc
     8881 cgccttcctg cattccctga ataaaactta gtccgagctt tgataccaaa agcggatctt
     8941 caccataaga tctgacattt ataattggat tatccggatt attatttatg tcgagtacgg
     9001 gtgcataatt ctgatgaata cctattgccc tgcattcttt agcgatctgc agtcccatct
     9061 gatatgcaag atctgtattt cctgtcgcgc cgagagccat gttgtttgga aataaactac
     9121 cgtcgttcag acgcatcttt gttcctcttt caaaatccgc tgacattaga agtggtgttt
     9181 cactcatttc ctgaagcata tttataagct ttgcttcctc gactgaatta cctttaaaga
     9241 aaataaatcc gcctaccttt tcattctcta ttaaattgct catcctttta aactctgacg
     9301 aactttcatt taccaaaaat ccatccgaat atgaaattat catttgagct atcttttctc
     9361 ttaatgtcat agatgcaagt ttatttgcaa cccatgtatt gtcggaattg tccaaaccaa
     9421 agaaattatc gctgacctgt tttgttttga taagttctgc cgatctaaga tttgatgttt
     9481 ccgcagaact gaatattatc agcattaatg aaattaataa aatcaccgga gtaaatattt
     9541 ttctcatgaa ataatttata attttatttt cttattcaaa cttaatcttt tttattttgt
     9601 gcgttattga gactgtcagt catagctgta aaatatctca taatctccat cggcagcggt
     9661 atgatcattg tagaattttt ctccgcggcg atctctgtca ttacctgaag atatcttagc
     9721 tgtaatgcat tctgactctt acctaaaata tccgctgctt cgccgagctt ctgtgctgac
     9781 ataaattcac cttctgaatt aatgatcttt gcacgcttat ctctttcagc ttcagcctgg
     9841 cgtgacattg ctctgatcat ttcctgagga aggtcaacgt ttttcaattc tacattcaat
     9901 acctgaattc cccatggctc agtttctttg tcaattagtt cgcgcagctt actgttcaca
     9961 agatctctgt gagtaagcag atcatcaagg tcaccctgcc cggtcacagt acgaagtgtc
    10021 gtctgtgata tcagtgaagt agcatacata aaatcctcta cctgaataat tgctctgttt
    10081 ggatctacta ctctgaaata aactacagca tttactttta ctgaaacgtt gtcctttgta
    10141 ataatatcct gtgaaggtac atcgagtgca atcgttctga gactcatctt taccatctta
    10201 tccataatag gaatcagaat gatcagcccc ggacctttga ctgctgagaa ttttcccagt
    10261 ctgaaaatta cgccgcgctc atattcattc agtaccctga aggctctgag tactgcaata
    10321 aataaaatga atcctaagat cccggcaaat acaaataata cgcctgctaa tgcttccata
    10381 ttgtgtaatt aaattatata atttttttaa atttattctg ataatttcag ggatcttcag
    10441 gctgtcgaaa taaaaaacaa attaaatttt cagtgccgtt gttggtgttc caaaagtttt
    10501 caaactcaaa ctttttattt acgactacac tttccaaaac ttttggtcac caacaacagt
    10561 gtacctgctg tttgaatctg caatttctaa gaacgcgttt ctgattagtg ccgtttattt
    10621 actcgttccc aatctcctga ttgggaacgt acctgaactt ttaatttatt tc
//
LOCUS       DAORVQ010000110        10392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3335_length_10392_cov_7.249976, whole genome shotgun sequence.
ACCESSION   DAORVQ010000110 DAORVQ010000000
VERSION     DAORVQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3335_length_10392_cov_7.249976"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1645)
                     /locus_tag="PLX80_09550"
     CDS             complement(2..1645)
                     /locus_tag="PLX80_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21073.1"
                     /translation="MKNIIILLLIFSCSEKAFSQLGFHWIDIQPGMINNVNANFSIAE
                     DLSGNFYTTGFEYLSNYSDLKIIKFNSSGNILWMRSYNGSANLSDGGNSIAIDRANNV
                     YISGYVTKTPVNTDIVLLKYNPYGDLIWDKEFSGSANVNDFGGSVVADDSGNVFLSGN
                     TSHTVSGSDITLIKYDSSGSFKWVKYYNSGLDTNDYDLKMKMDRNGNLCIAGYNNNST
                     GNNTKTLALKFDHDGNLLWNRIYNGPKNGFNTPHDFIVDRSDNMIITGCSPGIGSGVY
                     DYITIKYDPDGEMKWLSRYNQQGTYYDVANSLAEGSDGSIYVTGVSGILINQSTFDIV
                     TIKYDSNGTQKWSAIYNGAGNNTDIGNKILIDENENVFVTGITSGQGTLKDIVIIKYD
                     SAGNQNDLLNFNSVNNLNENIYEGIISSNGELILAGNVQTGNTSYSALTMSISTKTNI
                     NVNSNLIPDDFKLYQNYPNPFNPKTIINYELQVTGEDLVNLKVFDVLGNEVAVLVNEK
                     QNAGSYSVEFDGSNFSSGIYLYTLSTNGISNTMKMIMLK"
     gene            complement(1765..3441)
                     /locus_tag="PLX80_09555"
     CDS             complement(1765..3441)
                     /locus_tag="PLX80_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21074.1"
                     /translation="MKYKLQFILVLLSFLFIQFIPNTSYSQFFKSWNSSYIISDSAAC
                     IGTALGKFNTAVTGKVKNPVSGSDVHTVFYNLWTGQPVRTVIYSTSQNEEPVDIKSSY
                     NQDMFYVLCKRSDSAGKSSLFIIKYNGFGDQIWTSEYNGPVNEEQFPVSIGLVHNFFG
                     NKFLYTTSKSYSKGDSTCKILTQKIDSSGNYLWSSYYTDTTLAGSTNDVPVNIELSED
                     NLNTIVAGITTDSAGISQTMILKYDSSGNVLWKQNWGTSVPRNNFVTCMDLKNNNFAV
                     GGIIQMGSTFDYFILKYDMNGNLLWSQTYDGPGNGNDYMSDIGIDNSGSVVVTGSIYI
                     DSVNKSDYGIVKYLSTGNFQWANSFNGPASGNDSAVCMVIDNFNCIFVTGKTLDTTGY
                     YCFGSKLYNQNGSTHWTRYFRSPALTGDNIPVGVSKQQFTNSFSLTGNTFGDTLNSFT
                     TLFYAGIIDGIEDNNSLNAGSYKLNQNYPNPFNPKTKINYELHVTSGEFVSLKIFNVL
                     GNEVATLVNEKQNRGRYSVEFDGSNFSSGVYFYKLTYSDISTTMKMILLK"
     gene            complement(3477..5210)
                     /locus_tag="PLX80_09560"
     CDS             complement(3477..5210)
                     /locus_tag="PLX80_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY21075.1"
                     /translation="MFFIFFGTGLENKLSSRLDNKLKYSPSESKQLVWIFFKDKVSNN
                     YSQKEISTYLTKRSIQRRMNRIKNSEITDDLDLPVNKDYIRELKEFGLEIKQISKWFN
                     AVSCYATKTQISMISGKSFINEIDLVSVYKNTGYHQTNDISGNGETLEQSDNNPVSTS
                     VINYGPSLNQSNLIHVPPVHDLGYTGKNVLIASFDAGFDNLQHTCFDRMREKGLRSYD
                     FVNGDSIVSNDTGRLGNGAHGTQTLSLICGFDPGWLISPAFDAEIILAKTENTESETP
                     LEEDNWIAAAEWADSLGADIITSSLTYLAFNAPHPSYTWEDMDGNTARITLAAEIAVS
                     KGIIVVSSAGNAGFNPDHNTLGAPADGKNVITVGAVTLGRQRIGFSSVGPTADGRIKP
                     DVMALGLSTYTAFPGSGNVGYLNIGTGTSFSCPMVAGVCALMLSANPLLTPQKVKELL
                     LNTSDKTGPPDNLTGWGIVNAYSAVLAAVKISNEIPGDFSIDQNYPNPFNPSTTISIH
                     LKRDATLSVIIFDILGKEKGHIVNKQFFSTGNHDLKYNPEVNGLQSGVYFYSLVANGA
                     LVDTKKFIYLK"
     gene            5484..6470
                     /locus_tag="PLX80_09565"
     CDS             5484..6470
                     /locus_tag="PLX80_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HQY21076.1"
                     /translation="MNLLILGGTIFLGKHIVNAAIENGHTVTLFNRGQHNPEWFPDLE
                     KIKGDRDGDLSGLKGRKFDGVIDTCGYIPRIVRKSAEFLKDSTGHYTFISSISVYNND
                     DVPGSNENSETGVITDDNVEKMTMENYGPLKVLCENEVMDVFGENSSIVRSGLIVGDG
                     DFSDRFTYWIHRISKGGKVIVPVSKINNVQFIDVKDLAEWTLKLTEEKISGVFNSTGP
                     LEALSLNTFVNKCKEFSGSDAEFIYMDEKFLLDEGVIPYQDLTLWLPEDSFGMNNVDI
                     SKAKNAGLKIRPLEKTLKDTAEYDLTRGPDFKLRTGLSDEKEKELLEKWESR"
     gene            6649..8892
                     /locus_tag="PLX80_09570"
     CDS             6649..8892
                     /locus_tag="PLX80_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HQY21077.1"
                     /translation="MYSKLTKFTGAFLLSVLMCSISFAGKKTSYDLFSIGDNTVNTEV
                     TSIVDNAVFMNINKDELKRICENRDLEINLSMPYGNNQFTNLTLQRFDILKADAKIVA
                     RTERGNEEVQLEDIAVSYTGKLNGYDNSLISITFTKEKVTGLLVSGNDNFILGNINDN
                     AGNETDTYILYKESDLKVKNDFNCFTEDNLSSTQIEEMRSTIISQLNDATPTDLYIAE
                     VALELDFATYNIYGGSIQNTTNYALALMAASSAIYMKEVNVKFIIPYVRVWTSPDPYV
                     GTNSNTLLNQFRAEWNANQQSVQRTIAHFITRRGGGLGGIAWVGVLCSSVNGGNGYGL
                     SGTDGPILPLPTYSWDVMVVSHEIGHNFGSPHTHNCGWVGGNIDSCYAVEGGCYTGPP
                     IAAVGTIMSYCHLNGSISLTKGFGPQPKALIRGNAENAFCMYVSPNDLQVGYPNGGES
                     FRTGNSTQIYWGTSLTGNLNIEFSADNGATWQTIQNNVSATSRIYDWTLPYIGTTSQG
                     KIRILNSANPNQGDTCDNSFNIILSYIPFQIVSPVTFTRIETTPTGTSQHRFDWTSTG
                     SHPSFRYGVKFRKLGSTVDYFYSSDNGGADTAAIFTNSFLDTLAQQLGTTGDSVRCSW
                     RSWAYNGFDSAQSSNSGIITIARTGVGINVISSVVPDKYELYNNYPNPFNPSTNIKFN
                     IAKSGITKLNIYDAGGRQVSSLVNENLSPGSYEFTFNAADLSSGVYFYTLNTSDFTAT
                     KKMLLIK"
     gene            complement(9026..10141)
                     /locus_tag="PLX80_09575"
     CDS             complement(9026..10141)
                     /locus_tag="PLX80_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HQY21078.1"
                     /translation="MKNLFTALFFTAILFISNSLINNTANACTDVRVIAKDGSVLVSR
                     SMEFGAAMDSRLVMRPRGSAFQSPAPDGPSGKSWTGKYGFVYLDGLGIDCVVDGMNEA
                     GLGFGALYLPGFAKYETVGQGMNEKALSNLQLGAWVLSQYSTVDEVRASLTEITVWGE
                     LLSVFGNDFVPLHYVIHDSFGTSIVLEWIEGKLTVYENTVGVMTNSPSYEWHMINLRN
                     YISLSPDNSNPVTVGGVTYPANGQGSGLFGMPGDPTPPSRFVQTVFALTASVTPKDAT
                     EALVLGQKLMNRVDLPNGLARDPKTGDSDITQWAVFRGLTEKKYYYRTYDNMTLTAID
                     LSTTDFTPGAPVRRMSIAATTPTVNYIDQNSMQVISK"
BASE COUNT         3378 a         1873 c         1768 g         3373 t
ORIGIN      
        1 actatttcag cattatcatt ttcatagtat ttgaaattcc attagtactc agtgtatata
       61 gataaattcc gctggaaaaa ttactgccgt caaactcaac cgaataacta ccggcattct
      121 gtttttcatt tactaatact gcaacttcat tcccaagcac atcaaatact tttagattta
      181 ccaaatcttc acccgtaacc tgtaactcat aatttattat tgtcttcgga ttaaatggat
      241 ttggataatt ttgatataat ttaaagtcat caggtatcaa attagaattt acatttatat
      301 ttgtctttgt tgatatactc atagttagtg cactgtaaga agtattgcca gtctgaacat
      361 taccggctaa aatcaattca ccatttgatg aaataattcc ttcataaata ttttcattca
      421 agttattaac tgaattaaaa ttcaggagat cattttgatt acctgctgaa tcatatttta
      481 taattacaat atctttcagc gttccttgtc cggaggtgat gcctgttaca aaaacatttt
      541 cattttcatc gatcagaatt ttatttccta tatcagtatt atttccggct ccattgtaaa
      601 ttgcagacca tttctgagtt ccgttagaat catatttaat agttacaata tcaaaagtag
      661 actggttaat gagaattccg gaaactcctg ttacgtaaat actgccgtca gatccttcag
      721 ctaatgaatt tgcaacgtca taatatgttc cctgctgatt atatctggac agccatttca
      781 tctccccgtc cggatcatat tttattgtaa tgtaatcata aacacctgaa ccaattccgg
      841 gactgcagcc ggttatgatc atgttgtcag atctgtccac aataaaatca tgaggtgtat
      901 taaaaccatt tttaggtccg ttataaatcc tgttccataa aagatttcca tcgtgatcaa
      961 actttaaagc taaagtttta gtattattac cggtagaatt attattatat cctgcaatac
     1021 acagattacc atttctatcc attttcattt ttaaatcata atcatttgtg tccagtcctg
     1081 aattataata tttcacccat ttgaaacttc cgctcgaatc atattttata agagttatat
     1141 ccgatcctga aacagtatga gaagtatttc cggataaaaa aacatttcct gaatcatctg
     1201 ctacaactga tcctccaaaa tcatttacat ttgcactgcc gctgaattct ttgtcccaga
     1261 tcagatcacc ataaggatta tattttaaca atacaatatc tgtattgaca ggagtttttg
     1321 ttacatatcc ggagatgtaa acattatttg ctctatcaat tgcaatcgaa tttcctccat
     1381 ctgatagatt tgcactgccg ttataacttc tcatccataa aatattaccc gatgaattaa
     1441 atttaattat ttttaaatct gaataattac ttaaatattc gaatcctgtt gtgtaaaaat
     1501 ttccgctgag atcttctgca atggaaaagt ttgcatttac attattgatc ataccgggct
     1561 gaatgtcaat ccagtgaaat ccaagctgac tgaaagcttt ttccgaacag ctaaaaatca
     1621 gcaataatat tattatattt ttcatgataa gttctataat ttttaatata gtcagagttt
     1681 aaaatcagca atgctaaatt actcaaatat aattctggaa aaaatattat tcttacaaat
     1741 gattgtaata tattatcatt taaattattt cagcagaatc attttcattg ttgttgatat
     1801 atcactataa gtcaatttgt agaaataaac tccgcttgaa aaattactcc cgtcaaattc
     1861 aaccgaatat ctgcctctgt tttgtttttc atttacgagt gtagctactt cattccccaa
     1921 tacattaaag attttaagac tgacaaattc accgcttgtc acatgaagct cgtaatttat
     1981 ttttgtcttg gggttaaatg gattcggata attttgattt aatttataac tgcctgcatt
     2041 tagagaatta ttatcttcaa tgccgtctat tatacctgca taaaaaagtg ttgtaaaaga
     2101 atttaatgta tcgccaaatg tattacctgt aagagaaaat gaatttgtaa actgctgttt
     2161 ggaaactcca acgggaatat tgtcgcctgt cagcgcagga gaacggaagt atcttgtcca
     2221 gtgagtcgaa ccattctgat tataaagctt tgagccaaag caataatatc cggttgtatc
     2281 taatgttttt ccggtaacaa aaatacaatt aaaattatct atcaccatac atacagcgct
     2341 gtcatttccg gatgcaggtc cgttaaaact atttgcccat tgaaaatttc ctgttgataa
     2401 atattttaca attccataat cactcttatt aacagaatca atataaatgc ttcctgtaac
     2461 tactacacta cctgaattat caattccaat atctgacata taatcatttc catttcccgg
     2521 accgtcataa gtctgtgacc atagcagatt tccattcata tcatatttca aaataaaata
     2581 atcaaaagtg ctgcccattt gtataattcc gcctacagca aaattattat ttttaagatc
     2641 catgcatgtc acaaaattat ttcttggtac tgacgttccc cagttttgct tccataacac
     2701 atttcctgaa gaatcatatt ttaagatcat tgtctgactt attcctgcag agtcagtagt
     2761 gatacctgct acaatcgtgt ttaaattatc ttcagataat tcaatattta cgggaacatc
     2821 attagtactt cctgctaaag ttgtatctgt ataatagctg ctccaaagat aatttccgga
     2881 agagtctatt ttttgagtaa ggattttgca ggttgaatca ccttttgaat atgattttga
     2941 agttgtatat aaaaacttat tcccaaaaaa attatgcact aatccaattg atacaggaaa
     3001 ctgttcttca ttaacaggtc cgttgtattc acttgtccag atctgatcgc cgaatccatt
     3061 gtactttata ataaacaaac tgctctttcc tgcgctgtct gaccttttac aaagcacata
     3121 aaacatatcc tggttatagc ttgatttaat atcaacaggt tcttcatttt gtgaagttga
     3181 ataaatcaca gttcttacag gctgacctgt ccacaaatta taaaagacag tatgcacatc
     3241 cgatccactg accggatttt taactttgcc tgtaacagca gtattaaatt tacccaaagc
     3301 agtaccaata caggctgcag aatcagaaat tatatacgaa ctgttccatg atttaaaaaa
     3361 ttgtgagtag gaagtatttg gaatgaattg aataaacaga aaagataaca ataccaaaat
     3421 gaattgtaat ttgtacttca taaaaaaaat ttaaagttag aaaaaaattt taggatttat
     3481 ttcaggtata taaacttttt tgtgtcgact aaagcaccgt ttgctactaa ggaataaaaa
     3541 taaactccgc tctgaagtcc atttacttca gggttgtatt tcagatcatg gtttcctgta
     3601 ctgaagaatt gtttatttac aatatgtcct ttttcttttc ctaaaatatc aaaaattata
     3661 acagaaagtg tagcatctct ttttaagtga atacttattg tagtggatgg attaaaaggg
     3721 tttggataat tctgatcgat tgaaaagtct cccggaattt cattactaat tttaacagca
     3781 gctagaacag cagagtatgc attgacaata ccccatcctg tcagattatc aggtggtcct
     3841 gttttgtctg aagtgttaag aagaagttct tttacttttt gaggtgtaag aagcggatta
     3901 gcagaaagca ttaaagcgca aactcctgca accataggac aggaaaacga agtacctgtt
     3961 ccaatattta ggtatccaac atttccggat ccggggaaag cagtataagt acttaagcca
     4021 agagccatta cgtcaggttt gatccttccg tcagcagttg gtccaacaga tgaaaatccg
     4081 atcctttgtc tgccgagagt aactgcacca acagtaatga catttttacc gtctgcgggt
     4141 gcaccgagag tattatgatc cggattaaaa cctgcattac ctgctgagga aacaactatg
     4201 attcctttac ttacagctat ctctgcagca agtgtgatgc gtgcagtatt tccgtccata
     4261 tcttcccatg tataggatgg atgaggagca ttaaaagcca gatatgtgag agaacttgta
     4321 ataatatctg ctccaagact gtcagcccat tcagctgcag ctatccagtt atcttcttca
     4381 agaggagttt cactttcagt attttcggtt tttgcaagaa tgatctcagc gtcaaaagca
     4441 ggagagatga gccagcccgg gtcgaatccg caaatcagag acaatgtctg cgtaccatgt
     4501 gcgccgtttc ccaatctgcc tgtatcgttt gaaacgatcg aatcaccgtt cacaaaatca
     4561 taactgcgta aacctttttc cctcatcctg tcaaaacatg tatgctgcag attatcaaag
     4621 cccgcatcaa atgaagctat gagtacattt ttgccggtat atccaagatc atgaaccggc
     4681 ggtacatgaa ttaaattcga ttgatttaaa gaaggtccgt aatttattac agaagtactt
     4741 acaggattat tgtctgattg ctcaagagtc tcaccattac cggaaatatc attagtttga
     4801 tgatatccgg tatttttgta aacacttaca aggtcaattt catttatgaa actcttaccg
     4861 gagatcatac taatctgagt tttagttgca tagcaggaaa cagcattgaa ccattttgat
     4921 atctgcttta tttctaaacc gaattcttta agttctctta tatagtcttt attaacaggc
     4981 aggtcaagat catctgtaat ttcagaattt ttaattctgt tcattcttct ttgaatagac
     5041 ctctttgtta agtatgtaga aatttctttt tgtgagtaat tatttgagac tttatcttta
     5101 aaaaagatcc agacaagttg ttttgattct gaaggtgagt attttaattt attatctagc
     5161 cgactgctaa gcttattctc aagccctgtt ccgaaaaaga tgaaaaacat tattacgaaa
     5221 caaattgtgg ctgatgtaaa atatttaggg ttgtttaatt tcatttctga aaacttatat
     5281 cataaattat tttgaatttc cagcaattgg gagaaaatga gttttatgat ttttataatc
     5341 taaaccgatt gatcaataaa aacaatacct atttttaaat taaatatttt ctgataaaag
     5401 cattaaaatt tcttataact tttattcaga tccttcgttg aaatcaccat tattttttta
     5461 attaatttat tatataacta cttatgaatc tacttatttt aggaggaaca atcttcctcg
     5521 gaaagcacat cgtaaatgca gctatagaaa atggtcatac tgtaactctt tttaaccgcg
     5581 gacagcacaa tcctgagtgg tttccggatc tcgaaaaaat caaaggtgac agagatggcg
     5641 acttaagcgg tcttaaagga agaaagtttg acggtgtaat agatacctgc ggatacattc
     5701 cccgtattgt cagaaaatct gctgaatttc tgaaagactc aaccggacat tatacattta
     5761 tctccagtat ttcggtatat aacaatgatg atgtacccgg aagtaatgaa aattcggaga
     5821 caggcgtaat tactgacgat aatgtagaaa agatgacaat ggaaaattac ggtcctctga
     5881 aagtactttg tgaaaacgaa gtaatggatg tctttggaga aaattcttcg attgtgagat
     5941 cggggcttat agttggagat ggtgattttt cagacaggtt tacatactgg attcacagga
     6001 tcagtaaagg tggaaaggta atagtgccgg taagtaaaat taataatgtt caattcattg
     6061 atgtaaagga tcttgctgaa tggacattga aattgactga ggaaaaaata tcaggtgtat
     6121 tcaattctac aggaccgctt gaggctctat ctttaaatac ttttgtaaat aaatgcaaag
     6181 aattttcggg aagtgatgca gagtttattt atatggatga aaaattttta cttgatgaag
     6241 gagtcatacc ataccaagat ctgacacttt ggctgccgga agattcattt ggcatgaaca
     6301 atgtcgatat ttcaaaagcc aaaaatgccg gcctgaaaat cagacctttg gaaaaaacac
     6361 tgaaagacac tgcagaatat gatcttacac gaggtcctga ttttaaactg cgtacaggac
     6421 tttctgatga aaaagaaaag gagttattgg agaagtggga gagcagataa cagcaaatat
     6481 ttgaagcctg ttaatattta taaaaacaaa cattaacccg gaacgctaac cctgaagatc
     6541 aggttacctg tgaacatcct cccccctccg gagattaaaa ttttaaaaac attttattat
     6601 aaaattttta tacctatttt tacaaactca attaaataaa aaaaatatat gtacagtaaa
     6661 ttaacaaaat ttacaggtgc ttttttgttg tctgttttga tgtgctcaat atcatttgca
     6721 ggtaaaaaaa catcttatga tcttttctcc atcggtgaca atactgtcaa tacagaagtc
     6781 acaagtatag ttgataatgc agtctttatg aatatcaata aagatgaact taaaaggatc
     6841 tgtgagaaca gagaccttga gatcaatcta tccatgcctt atggcaataa tcaatttacc
     6901 aaccttacac ttcagagatt tgatatcctc aaggccgatg caaaaatagt tgcaaggact
     6961 gaacgaggta atgaagaggt tcagcttgaa gatattgctg tctcttacac cggtaaactg
     7021 aatggttacg acaattccct gatctcaatt acatttacaa aagaaaaagt aacaggactt
     7081 ttagtttcgg gaaatgataa ttttattctc ggaaatataa atgataatgc aggtaatgaa
     7141 actgatacct acatccttta taaggaaagt gatctgaaag taaaaaacga ttttaactgt
     7201 tttacagaag ataatctttc ttcaacgcaa atagaagaaa tgagaagtac tatcatcagt
     7261 caattaaatg acgctacacc aaccgatctt tacattgctg aggtcgctct tgagcttgac
     7321 ttcgcaactt ataatattta cggaggatct atacagaata ctacaaatta tgctttggca
     7381 cttatggctg cttcatcagc tatttatatg aaggaagtta atgtgaaatt tattatccct
     7441 tatgtcagag tctggacttc tcctgatcca tatgttggaa cgaattcaaa tactttgtta
     7501 aaccagttca gagctgaatg gaatgcaaat caacagtcag ttcaaagaac tatcgcacat
     7561 ttcataacaa gaagaggcgg tggtctggga ggaattgcat gggttggtgt tttatgttca
     7621 tctgtaaacg gcggtaacgg atacggactg agcggaactg acggtcctat tctaccactt
     7681 ccaacttatt catgggacgt aatggtagtg tcacatgaga tcggacataa ttttggttca
     7741 ccgcacacac acaattgcgg ctgggtcgga ggaaacatcg acagttgtta tgcggtggaa
     7801 ggtggatgtt ataccggtcc tcctattgca gcagttggaa ctatcatgag ctattgtcat
     7861 cttaacggtt caattagttt gaccaaagga ttcggtcctc agccaaaagc tttgataaga
     7921 ggaaatgctg agaacgcttt ctgtatgtat gtttcaccaa atgaccttca ggtcggatat
     7981 ccaaacggcg gtgaatcttt cagaacaggt aattcaacac agatatactg gggtacatct
     8041 ctgacaggta atttaaatat agaattctca gcagacaatg gtgcgacctg gcagacaatt
     8101 caaaataatg tctctgccac aagtaggatc tatgactgga ctttgcctta tataggtacg
     8161 acctcacagg gaaagatcag aatattgaat tcagctaatc ccaatcaagg tgatacctgt
     8221 gataattctt ttaatataat actgtcttat attccgtttc aaattgtatc acctgttaca
     8281 ttcacaagga tcgagacgac acctaccggt acttctcagc acagatttga ctggacaagc
     8341 acaggatctc atccttcatt cagatatggg gtaaaattca gaaaacttgg cagcacagtt
     8401 gattattttt attcgagtga taacggcggt gcagatacag cggcaatttt cacaaacagc
     8461 ttccttgaca ctcttgctca gcagcttgga actacaggtg attctgtaag gtgttcgtgg
     8521 agaagctggg catacaacgg atttgattca gcgcagtctt caaacagcgg tatcattact
     8581 attgcaagaa caggcgtggg aataaacgtg atctcatctg tcgtccctga taaatatgaa
     8641 ctttacaaca attacccaaa tccatttaac ccgtcaacaa acataaaatt taatattgca
     8701 aaatcaggca ttactaaatt gaatatttat gatgcaggtg gaagacaggt cagttcactt
     8761 gtaaatgaga atctgagtcc cggaagttat gagttcactt tcaatgcagc agatctgtcg
     8821 agtggagtgt atttctatac tttaaataca tctgatttta cagccacaaa gaaaatgttg
     8881 ttaataaaat aatatcatga gattcagata ttagattaaa aatttatctt tattttaaca
     8941 aaaaccctgt gagaattttt cttacagggt tttttattta aaattaaact atcactgaat
     9001 cataagactt ttcgcaattg tcctattatt ttgagatcac ctgcatcgaa ttctgatcta
     9061 tataatttac agtcggagtt gttgctgcaa ttgacattct tcttaccggt gcgcccggag
     9121 tgaagtctgt tgtactaaga tcaattgccg tgagagtcat attatcataa gtgcggtaat
     9181 aatatttttt ttctgtaaga cctctgaata ccgcccactg tgtaatgtca ctgtctcctg
     9241 tttttggatc acgtgcaagt ccatttggaa gatcgacacg gttcattaat ttctgtccga
     9301 gtacaagagc ttcagtagcg tcctttggag taacggaagc agtgagagca aatactgtct
     9361 gcacaaatct cgaaggagga gtcggatctc cgggcattcc gaataatcct gatccctgac
     9421 cattcgcagg ataagtgact ccaccaactg taacaggatt tgaattgtca ggtgacagac
     9481 ttatataatt tcttaagttt atcatgtgcc attcgtaaga aggtgaattt gtcatcacgc
     9541 ctacagtatt ttcatagaca gtgagtttcc cttcaatcca ttcgagcaca atgctagtac
     9601 cgaatgaatc atgaattaca taatgcagcg gtacaaaatc atttccaaaa acagaaagaa
     9661 gttctcccca gactgtgatc tcagtaagtg aagctctcac ttcatcaaca gtagaatact
     9721 gtgaaagaac ccacgcacca agctgaagat ttgataaagc tttttcattc atcccctgtc
     9781 cgacagtttc atatttagca aaaccgggga gatacaaagc gccaaatcca agtcccgctt
     9841 cattcattcc gtccactacg cagtcaatcc caagtccgtc aagatataca aatccgtact
     9901 ttcctgtcca gcttttacct gaaggtccgt caggtgcagg agattgaaat gctgatccgc
     9961 gcggacgcat tacaagacgt gaatccattg ccgcaccgaa ttccatcgaa cgtgagacaa
    10021 gtacagatcc gtcttttgca attaccctta catcagtgca ggcattggct gtattgttta
    10081 ttaatgaatt agaaataaac agtattgctg taaaaaataa agcagtgaac aaatttttca
    10141 ttttttgatt ttttatttgt aaaaaataaa tgcttcctta ttgtttccag gaaggtttaa
    10201 ataagtgttt aattttgata taattttata tatgtaaatt ttttaaaaac tgaaattgta
    10261 attagctgtt ttaattttat taaaatacat agtaaagtta aatttataaa ttaattttta
    10321 actcccttaa ataaaacctc tgtacacgac aaaacttttg aataaaaaat aaaaatctga
    10381 tccaaaattg tt
//
LOCUS       DAORVQ010000111        10318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3367_length_10318_cov_5.700088, whole genome shotgun sequence.
ACCESSION   DAORVQ010000111 DAORVQ010000000
VERSION     DAORVQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_10318_cov_5.700088"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(416..1639)
                     /gene="dnaJ"
                     /locus_tag="PLX80_09580"
     CDS             complement(416..1639)
                     /gene="dnaJ"
                     /locus_tag="PLX80_09580"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQY21079.1"
                     /translation="MTKRDYYEILSVSRTSTTDEIKTSYRKLAMKYHPDRNPNDNEAE
                     EKFKELAEAYEILSNTGKRQRYDQFGHQGINGGGSQGFSDINDIFSQFGDIFGGRSGG
                     SGGSIFDEFFGGGGGSSRRQNNNRGSDLKIALKLSLEEIADGVEKTVSLKKLKTCTTC
                     NGTGAKGEKGYSNCTHCNGSGEVRHVSRSIFGQVVNVSMCTYCSGEGRIIKEKCSDCH
                     GEGLKKEETKIKLDVPAGVQEGNYIPLRGQGNAGKRGGQNGDLLVFIEEEEHKYFVRD
                     EDDIYYDLEISIVDAVVGADLVVPTLNGKVKLTVDPGTQSGSFLRMKGKGIKTLNGYG
                     RGDEIIRINVHIPKKINSDEKKILNDLSRSENFKPRASKFKDDDNEDNNSKNSKSSDK
                     KSKKQDKGFFKTVFS"
     gene            complement(1659..2234)
                     /locus_tag="PLX80_09585"
     CDS             complement(1659..2234)
                     /locus_tag="PLX80_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQY21080.1"
                     /translation="MSNKTKHNIKIKDEENMNENEEAVTDIEDADTEKEEISTNEISP
                     EQEIARLKDTLLRKAAEFENYKRRTDTEISNYIKYASENLIKELLPVYDDLGRSVDSV
                     NKGETKDFDTLKKGTALVYEKFKGVLEREGLTEINSVGNEFDVDLNSAMMMVPRDDVK
                     PNTILEVIEKGYKLKDKVIRHEKVLVSSEPN"
     gene            complement(2268..3305)
                     /gene="hrcA"
                     /locus_tag="PLX80_09590"
     CDS             complement(2268..3305)
                     /gene="hrcA"
                     /locus_tag="PLX80_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQY21081.1"
                     /translation="MITLTDREKEVLRYVVDNFISSAAPIGSRSVSKKTDLNLSSATI
                     RNVMSDLEEMELLDTPHTSAGRIPTDKGYRYYVNSLMGQGSISEKEEFLFKGFFNDKN
                     LNILENQEIFLETSKLLGKISHLLTVVSQPKIAKGIFEKLEFVNVASNKILVVLKIKS
                     GIIKTVIMEITSEIPKIKLEKLSSFINEKLSGLTLELIRETYAERISDYRHKEPELIQ
                     SFMNSFEKLYADEESGNKIYIGGTGDLILQPEFEDPKNFTDIIKLAEDKNLVIHIFDN
                     DKSLENKVQISIGGENKDAKLKNYSVITTSYNLGEVKGNIGVIGPKRMDYAKMISLLE
                     YTSKLINEFSN"
     gene            complement(3362..4201)
                     /locus_tag="PLX80_09595"
     CDS             complement(3362..4201)
                     /locus_tag="PLX80_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQY21082.1"
                     /translation="MKAAVAQTTCVLGDLNKNISHHLEFCDEAIKNKADLIVFPELSL
                     SGYSLKDLNYVIAVNLKETKLLDELKKKSKKISIICGLAEEGDDYGIYNTGIFISDGE
                     IKFSHRKVYPPTYGLFEEFRYFSKGKECKAHETKFGKVGLLVCEDMWHMSLPYTLAYD
                     EAKILIGIAASPTRLAANHEGFKNYEVNSEHHRTFARMLSSYFLFSNRVGYEDGVNFW
                     GGSEIIDPFGNVLAAAKLFEEDIIYADIDMEEVKRSRHQARHFLDEDLELTIRNLSKI
                     QKK"
     gene            complement(4198..5076)
                     /locus_tag="PLX80_09600"
     CDS             complement(4198..5076)
                     /locus_tag="PLX80_09600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21083.1"
                     /translation="MPDNKKVSVIIVTWNSSDEIEGCINSIINSQRINSSEFEIIVID
                     NNSTDDTKIKLDQIKKNLYSNIILIYNKENVGFTKACNQGIEIITGENILILNPDTEI
                     VDDAIDKMAAKLNSDKSIGAVAPKLLNKDGSIQKSCRTFPEYSDMFFEMTLLSKLFPK
                     SKKVSRWKMNYFDHDKECKVDQPMAAALMIKKSVLDNIDNFDERYKMFFNDVDICKKI
                     YDSGKSIIFYPEAVVIHIKGVSIYKDREKMIRIWNKDCAEYFKKYHNNVLLYNLLKAG
                     LTVSGAVRILLYKLTK"
     gene            complement(5069..6028)
                     /locus_tag="PLX80_09605"
     CDS             complement(5069..6028)
                     /locus_tag="PLX80_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21084.1"
                     /translation="MSNKILTLLLFFVPLLIVSCQKTESEGSGNIKNTDSGNISSAGI
                     KESGKEFDVRYNSPRKIYNIVKEDVNNDGNREIFVMSILKDTADKFSSYYNFDMVEVF
                     SLDRSSEKYVKILTDTVDYSTEISFENIPKDESKLIFIKTNTGGNDVLNSEGMFVYQM
                     NDSANIEMIKYFETGSPDIKDLNNDGNKELIVSDQFLGLMPQVNAVSFIREIYTMKDH
                     KLVLSNEEFPEVYDKKINELIENFNGLKRKVEMGMQPMDLSYPLYREAAEIFVNYYSK
                     GDMQGLKNFWEKEKDSLKKNIPNDEYQDLNSFISKALPSSSNA"
     gene            complement(6034..8496)
                     /locus_tag="PLX80_09610"
     CDS             complement(6034..8496)
                     /locus_tag="PLX80_09610"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQY21085.1"
                     /translation="MKNFFIITITFLSCTVSAQVGIPLPERKSNFDVLNIDINLELDV
                     KGKFLSGIVTSEITPTVNDLEIIELDAAALNINSVEDQSGNILKFEYTDNKLKIYTGE
                     KYSPGEKLKVSVNYTCSPQRGMYFIYPSELNPSMPYQVWTQGEDENNRYWLPIYDYPN
                     DKATVSLKVTVDDNFKTLSIGELISSEKSVETGKRTDHWVINKPVSSYLIMIAAGEFN
                     ILEKMYGNIPIQSYTDQNINLDEAEYTFRNVPEMTRYFSELFQYEYPWNKYAQVVVED
                     FMHGGMENVTATVLNKNLIYNSSVENEFGTEGTISHELGHQWWGDLITCRSWSEIWLN
                     ESFATYSDVIWDEKVYGKDEYDYNLVLYGDAALRFDSISGRYPVWAGYGRYTTNVYSK
                     GAVILNTFKYILGDKFFPSLGSFLKDNEFKEVETTDLINSINKTTGKDYKWMFDQWIW
                     KAGQPEFFVSYDFDENKNEIDLKVKQMQKEDSLTPVFKMPIEVRVKERNNDEVHKIEI
                     TEREQTFRLPCTSAPEFVVFDYGNNILDKLFFDKPFDDWKNQLLQSENAVDRIMALRG
                     LERFLKPDLSETAGKPVITIDQSETLKLFENILNNDKYWGVRIEAAKILGNNSVINFN
                     IDVLKNSYDIQTNSKVKRQIIKSTGSSRNNMNSDFIIEKIKNEKNDYIVADGISSLAI
                     CMDKKDIPEFVIPFAERNSHRDVIQRSVIRALDSANIDGTDQQIKSVIMNIAFGKDTE
                     GRLRSAALNSLAKYARDSDVKDSALKYLDYNFIYVKNSMLNLLALSGDRSLIPVLNEE
                     LINCTNPSIKKYYENALAKLNK"
     gene            complement(8496..10157)
                     /gene="nadB"
                     /locus_tag="PLX80_09615"
     CDS             complement(8496..10157)
                     /gene="nadB"
                     /locus_tag="PLX80_09615"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQY21086.1"
                     /translation="MTTDFLIIGSGIAGLSLALKLSAFGKVIVITKKQKSESNTNYAQ
                     GGIAAVMSDDDNFELHINDTLDCGAGLCDIKAVRKIVEEGPDMIRELTEFGAKFSNDK
                     GTLLLGKEGGHSKNRIIHAKDLTGKEIERALLSQISKNKKIRLFEYYFAVDLLTNKNL
                     KKKKDKEQRCYGIYALNVKTDKVEKIIAKKTILATGGAGQIYLHTTNPAIATGDGIAM
                     GFRAGCEIANMEFIQFHPTALYDPESDDNFTNNGQVFLISEAVRGAGAVLKTNRGKEF
                     MKKYDKRGSLAPRDIVARAIDNEMKKSGDTHVMLDLSNVGKEKILKEFPNIYKYCLTK
                     GIDITKQMIPVVPAAHYTCGGLKTDLRGRTSLTDLYASGEVTMTGVHGANRLASNSLL
                     EAMVFSNAIYEDIKNIYQNSDKKVFKNLTDKEYPAKLKDNIYDWDDDGMENTDEWILI
                     SHNKKEIKEVMSDYVGIVRNMLRLERAYRRIKMMREEIKEFYDKTKVNVDLLELRNMV
                     TVAYLVIQSAMLRKESRGLHFMSDFLQKDDKNFLNDTIIVKDQYL"
BASE COUNT         2998 a         2075 c         1393 g         3852 t
ORIGIN      
        1 aaaattataa acgcgtttat taaattaaat atcgcgttat tgaaaaataa aattgtgata
       61 atgtcaaaaa aacagtggta aatatctttg agcttttgtt ggagttcctg atattttgat
      121 aaatcctgtg tacagattac aatataaatg aactcaaact ttcatttccc cagtaagctg
      181 agaaaaaagc acaatgatca ttatacaata ttgccggaga caaaacaatt tgaatcactt
      241 ccagatttat tctacacatt aaattttgca tacttttttg gaactctatt aacgttaaat
      301 aatatactat ttgaaatttg aaatttaaaa tttaaaattt aaaatttaaa attaaaaaag
      361 ctgtgctttg taaatatcta caaagtacag cttttatata tatctgaatt tcttattagc
      421 tgaacaccgt tttaaaaaat cccttatcct gtttcttgga tttcttatcc gaagatttac
      481 tatttttaga attattatct tcgttatcat catctttaaa tttcgaagca cgcggcttga
      541 aattttccga acgacttaaa tcattcaata tctttttctc atcggaattt attttctttg
      601 gaatgtgaac atttatcctg atgatctcat cacctcttcc ataaccgttc agcgttttaa
      661 ttcctttacc tttcattctg agaaaacttc cggactgtgt gccgggatcc actgtcagct
      721 ttactttgcc atttaatgtc ggcactacaa gatcagctcc tacgactgca tctactatac
      781 ttatttccag atcataataa atatcatctt catctctcac aaaatattta tgctcttctt
      841 cttcaataaa aacaagcaga tcaccattct gtccgcctct cttgcctgca tttccctgac
      901 ctctgagcgg aatataattt ccttcctgaa ctcccgcagg tacgtcgagt ttaattttcg
      961 tttcttcttt tttcaatcct tcaccgtgac aatcactgca tttctcctta atgatcctgc
     1021 cttcgccgct gcaatatgta cacatgctga cgtttaccac ctgtccgaaa atggatctgg
     1081 aaacatgtct gacttcacct gagccgttac agtgcgtaca gtttgaatat cctttctcac
     1141 cttttgcacc ggttccgtta caggtagtac atgtttttaa ttttttcaga ctgacggttt
     1201 tttcgacacc gtctgcaatt tcttcaagac taagtttcag agctattttc agatcactgc
     1261 ctctgttatt attttgcctt ctcgaactac cacctccgcc gccaaagaat tcatcaaaga
     1321 tacttccacc gcttccgccg cttcttcctc cgaatatatc tccaaactgc gagaatatat
     1381 cgttgatatc actgaatccc tgacttcctc caccatttat accctgatga ccgaactggt
     1441 catatctctg acgctttccg gtatttgaaa gaatctcata tgcttcagca agttctttaa
     1501 atttttcttc agcttcattg tcattcggat ttctgtccgg atgatatttc atagcaagct
     1561 ttctgtatga agtttttatc tcgtcagtcg ttgatgtcct gctgactgat aatatttcgt
     1621 aataatctct ttttgtcatt ttagtttaat tcctttcttt aatttggttc acttgatact
     1681 agaacttttt cgtgtcttat cactttgtcc tttaatttat aacctttctc aattacctcc
     1741 aatattgtgt tcggctttac gtcatctctg ggtaccatca tcatcgctga attcagatca
     1801 acatcaaatt catttccaac agaattaatt tcagttaacc cttctctttc aagcactcct
     1861 ttaaattttt cataaactaa agccgtccct tttttcaaag tatcaaagtc ttttgtctca
     1921 cctttgttta ccgaatccac tgacctgccg agatcatcat acaccggcaa aagttctttg
     1981 atcagattct cagacgcgta tttgatatag tttgaaattt cagtatcagt tcttcttttg
     2041 taattttcaa attcagccgc tttccttaaa agagtatctt tcagtcttgc gatttcctgt
     2101 tcaggagaaa tttcatttgt agaaatctct tctttttctg tgtcagcatc ttcaatgtct
     2161 gttactgctt cttcgttttc attcatattc tcttcgtcct taatctttat attatgttta
     2221 gttttattac tcattacatt ttcctttctt tactttttaa acttaatcta attagaaaat
     2281 tcatttatta attttgatgt atactcaagc agtgatatca tttttgcata gtccattctt
     2341 ttcggaccaa ttactccaat gttacctttc acttccccaa gattatatga agtcgtgatc
     2401 acactgtaat tcttgagttt agcatctttg ttctcgccac caattgagat ctgaacttta
     2461 ttctcaagag acttatcatt atcaaaaata tggataacaa gatttttatc ttcagccagt
     2521 tttattatgt ctgtaaaatt tttcggatcc tcaaattcag gctgtagtat cagatcaccc
     2581 gtcccgccaa tataaatttt atttccactt tcctcatccg cataaagctt ttcaaaagaa
     2641 ttcataaaag actgaatcag ctcaggttct ttatgtctgt aatcactgat tctttcagca
     2701 taagtctctc tgataagctc taaagtgaga cccgataatt tttcatttat aaaagaagac
     2761 agtttctcaa gtttaatttt agggatttca cttgtgatct ccattataac agtttttatg
     2821 atacctgatt taatttttaa gacaaccagt attttatttg aagctacatt tacaaattct
     2881 aatttttcaa atattccttt agcgattttt ggctgggata caactgtaag taaatgcgat
     2941 attttaccta acagctttga tgtctcaaga aatatctctt gattttccag tatgttcagg
     3001 tttttatcat taaaaaatcc tttgaaaaga aattcttctt tttctgaaat acttccctgt
     3061 cccatcagtg aattcacata atatcggtaa cctttatcag tcggaattct gcctgcagaa
     3121 gtatgcggag tgtcgagcag ttccatttcc tcaaggtcgc tcattacatt ccggatagta
     3181 gctgaagaca agttcagatc agtttttttc gaaacgcttc tcgaaccaat tggcgcagcg
     3241 ctgctgataa aattatctac aacatatctt aatacttctt tttctctatc tgttaaagtg
     3301 atcatttttt atttggacaa ataaagtact tattttaact tttttttaaa attccttttt
     3361 tttacttctt ttgaattttg gaaagattcc tgatcgtcag ttccaggtct tcatcaagaa
     3421 aatgtctcgc ctgatgcctg gatcttttga cctcttccat atcgatatct gcatatatta
     3481 tatcctcttc aaataatttt gctgctgcaa gtacattccc gaaagggtct ataatctcac
     3541 tgcctcccca aaagttcaca ccgtcttcat atccaaccct gtttgaaaaa agaaaataac
     3601 tcgaaagcat tcttgcaaat gtcctgtgat gctcagaatt aacctcataa tttttaaagc
     3661 cttcgtggtt tgcggccagt ctggtaggcg atgcagctat tccgattagt atctttgctt
     3721 cgtcatatgc aagtgtataa ggaagagaca tatgccacat atcttcgcat acgagtaacc
     3781 ccacctttcc aaattttgtt tcatgcgcct tacattcttt tccttttgag aaatatctga
     3841 actcctcaaa cagcccgtaa gtcggcggat aaacctttct gtgtgaaaat ttaatttcac
     3901 catcagaaat aaatatcccc gtattataga ttccataatc atcaccttcc tctgcaagac
     3961 cgcagatgat tgagatcttt ttgctttttt tcttcagctc atccagtaat ttagtctctt
     4021 tcagatttac tgcgatcaca taattcaggt cttttaaaga atatccactg agtgaaagtt
     4081 caggaaatac tattaaatcc gctttgtttt ttattgcctc atcacaaaat tcaagatgat
     4141 gacttatatt tttattcaga tctccaagta cgcaggtcgt ctgcgctact gctgctttca
     4201 tttagttagt ttatataata aaattcttac tgctcctgat actgtcaaac ctgccttaag
     4261 cagattgtat aataaaacgt tgttatgata ttttttaaaa tattctgcac aatctttatt
     4321 ccagattctt atcatctttt ctctgtcttt atatatgctc acacctttta tatgaatgac
     4381 caccgcctca ggataaaaga ttatactttt tcctgaatca taaatttttt tacaaatatc
     4441 tacatcgtta aaaaacattt tataccgttc atcaaaatta tctatattat cgagtacact
     4501 ttttttgatc atcagagctg ctgccatcgg ttgatctact ttacactctt tatcatgatc
     4561 aaaataattc atcttccatc tgcttacttt cttacttttg ggaaataatt ttgacagcaa
     4621 agtcatttca aaaaacatat ccgaatattc cgggaaagtc ctgcaggatt tttgtatgct
     4681 gccgtcttta ttcaaaagct tcggtgcaac tgcaccaatg cttttatcag aattaagttt
     4741 tgcagccatt ttatcaattg catcatctac aatttccgta tcgggattta atatgagaat
     4801 attttctccg gtaataattt caattccctg attacatgct ttagtaaatc ctacgttctc
     4861 cttattatag atcagaataa tattactgta taaatttttc ttaatctgat ctagcttaat
     4921 ctttgtatca tcagttgaat tattatcaat tacaataatt tcaaactcgg agctattaat
     4981 tctttggctg tttataattg aattaataca gccttcaatc tcatctgaac tgttccaagt
     5041 gacgattata accgatactt ttttattatc aggcattact tgatgatggt aatgctttcg
     5101 aaataaaact attcaggtcc tgatattcat catttggaat atttttttta agggaatcct
     5161 tttctttttc ccagaaattc ttaagcccct gcatatcacc tttggaataa taattcacaa
     5221 atatctctgc cgcttccctg tatagaggat atgacaagtc cataggctgc attcccattt
     5281 caacttttct ttttaatccg ttaaagtttt ctattaattc attgattttc ttatcataca
     5341 cttccggaaa ttcctcatta cttaaaacta atttatgatc cttcattgta tatatttctc
     5401 ttataaaact tacagcattc acctgaggca ttaatccaag gaactggtca cttacaatta
     5461 attctttatt tccgtcgtta ttaagatcct taatatcagg tgagcctgtc tcaaaatact
     5521 tgatcatttc tatgtttgcc gaatcattca tttgataaac aaacattcct tcagaattca
     5581 ggacatcatt cccgcctgtg tttgttttga taaaaataag cttactttca tctttcggaa
     5641 tattttcaaa agatatttca gtggaataat caactgtatc tgtaagtatc tttacatatt
     5701 tttccgatga ccggtctaat gaaaagactt caaccatatc aaaattataa taactgctga
     5761 atttgtcggc agtatctttt aaaatactca tcacaaatat ctcacggtta ccgtcattat
     5821 ttacatcttc ttttacaatg ttatagatct tccttggtga attatatctg acatcaaatt
     5881 cttttccact ctctttaata cctgcagaag atatattccc gctgtcagta tttttaatat
     5941 tgcctgagcc ttcactttct gtcttttgac atgaaactat taaaagaggg acaaaaaata
     6001 aaagcagtgt taatattttg tttgacaaat ttttcattta tttaatttag ctaaagcgtt
     6061 ttcataatat tttttgatac tcggattagt acaattaatt aattcttcat tcaaaactgg
     6121 aatcagagat ctgtcaccag ataatgccag cagatttaac atagaatttt ttacatatat
     6181 aaaattataa tcgagatatt ttaaagcaga atcttttaca tcactatcac gtgcatactt
     6241 tgccagtgaa tttaaagctg ctgatctcag cctgccttct gtatcttttc caaatgctat
     6301 gttcataatt acacttttaa tctgctgatc tgtgccgtca atatttgccg aatcaagtgc
     6361 ccggattacc gacctttgga tcacatctct gtgagaattt ctttcagcaa aaggaattac
     6421 aaattcaggt atgtcttttt tatccataca aattgcaaga gatgaaattc cgtcagctac
     6481 aatataatca tttttttcat ttttaatttt ctctatgata aaatctgagt tcatattatt
     6541 tcgagagctt cctgtggatt ttattatctg cctttttact tttgaatttg tctgaatgtc
     6601 ataagaattt ttaagcacat caatattgaa attaataaca gaattattgc caaggatctt
     6661 tgcagcttca attctaacac cccagtattt atcattgttt aaaatatttt caaaaagctt
     6721 aagtgtttca gactgatcta tagtaatcac tggttttccc gctgtttcag ataaatcggg
     6781 ttttaaaaat ctctcaagtc cccgaagagc cattattctg tcaactgcat tttcactttg
     6841 aagaagttga tttttccagt catcaaacgg tttgtcaaaa aataatttat cgagaatatt
     6901 atttccgtag tcaaacacaa caaactcagg agctgatgta catggtaatc tgaaagtctg
     6961 ttctctttct gttatttcaa ttttatgaac ttcatcatta ttccgttcct taactctaac
     7021 ctcgatcggc attttgaata caggagtaag tgaatcttct ttctgcatct gtttcacttt
     7081 cagatctatc tcatttttat tttcatcaaa atcataactc acaaaaaatt caggttgtcc
     7141 tgctttccat atccactgat caaacatcca tttataatct ttacctgtag ttttatttat
     7201 ggaatttata agatcagtag tttcgacttc tttaaattca ttgtctttta gaaatgatcc
     7261 aagagaaggg aaaaatttgt cacctaaaat atatttgaat gtatttaaaa ttacagcacc
     7321 tttggaatat acgtttgttg tatatcttcc atatcccgcc cacacaggat atctcccgct
     7381 gatactgtca aatctcaatg cagcatcacc atacaaaact aaattgtaat catattcatc
     7441 tttcccataa accttttcat cccatataac atctgagtaa gttgcaaagc tttcattcag
     7501 ccagatctca ctccagctcc tgcaagtgat caggtcgccc caccactgat gaccgagttc
     7561 gtgagagata gtaccttctg ttccaaattc attttcaaca gaagaattat atatcagatt
     7621 tttatttaag actgttgcag tgacattctc cattcctcca tgcataaaat cctcgacgac
     7681 aacctgagca tatttattcc acggatattc atattgaaat aattcgctga aataacgagt
     7741 catttcaggt acatttctga aagtatattc tgcttcatct aaatttatat tctgatcagt
     7801 ataagattgt atgggaatat tcccatacat tttttctaat atattaaatt ctccggcagc
     7861 gatcattatc agataggaag aaaccggttt attgatgacc cagtgatcag ttctttttcc
     7921 ggtctcaaca gatttttccg aagagataag ttcaccaata gaaagagttt taaaattatc
     7981 atcgacagtt actttcagac ttacagttgc tttatcattc ggataatcat agatcggcag
     8041 ccagtacctg ttattttcat cttcaccctg agtccagacc tgataaggca ttgacggatt
     8101 taattctgaa ggataaataa aatacattcc tctctgcgga ctgcaggtat aattcacgct
     8161 gacttttaat ttctcacccg gagaatattt ttcgccggta taaattttaa gcttattatc
     8221 tgtgtattca aattttaaaa tatttcctga ctgatcttca acagaattta tattcaaagc
     8281 tgcagcatca agttcaatta tttcaagatc attaacagtt ggtgttatct ctgacgtcac
     8341 aattcctgac aaaaattttc cttttacatc cagctctaaa ttaatatcaa tattcaaaac
     8401 atcaaaatta cttttccttt ccggtaaagg gattccaacc tgagcggaga cagtacagga
     8461 aagaaatgta atagtaatta tgaaaaaatt tttcattata aatattggtc tttaacaata
     8521 attgtatcat ttaaaaaatt cttatcgtcc ttttgtaaaa agtctgacat aaaatgcagc
     8581 cctctggatt cttttctgag cattgcagat tgaataacaa ggtaagcaac tgtaaccata
     8641 tttctaagct ccagcagatc tacatttact tttgtcttat cataaaattc ctttatctct
     8701 tccctcatca tcttaattct tctgtatgct ctttcaagtc tgagcatgtt tcttactatt
     8761 cccacataat cactcatgac ctctttgatc tcttttttat tatgtgagat aagaatccac
     8821 tcatctgtat tttccattcc gtcatcatcc cagtcataaa tattatcttt aagcttcgcc
     8881 ggatattctt tatcagttaa atttttaaat acttttttat cagaattttg ataaatattt
     8941 tttatatcct catatatggc atttgaaaat accattgctt caagcagaga atttgaggca
     9001 agtctgttag caccgtgtac gccggtcatc gttacttcac cgcttgcata aagatctgtc
     9061 agacttgttc tgccgcgcag atcagttttt aaaccgccgc atgtatagtg agctgcagga
     9121 acgacaggta tcatttgctt agtaatatca attcctttgg tgagacaata tttatatata
     9181 ttcggaaatt cttttaatat cttttcttta ccaacatttg acagatccag catcacatga
     9241 gtgtcaccgc tttttttcat ttcattgtcg atagctcttg cgacaatgtc tctcggagcg
     9301 agtgaacccc gtttgtcata ttttttcata aactcttttc ctctgttcgt tttaagtact
     9361 gcacctgctc cgcgtactgc ttctgatatc agaaatacct gtccattatt agtaaaatta
     9421 tcatcacttt cagggtcata taaagctgta ggatgaaact gaatgaattc catatttgca
     9481 atttcacaac ctgcgcggaa acccattgca ataccgtcac cggttgctat cgccggatta
     9541 gtggtgtgaa gatatatctg tccagctcca cctgtggcga gaattgtttt ctttgctatg
     9601 atcttctcga ctttatcagt ctttacattt aatgcataaa taccgtaaca tctctgctct
     9661 ttatcttttt tctttttaag atttttattt gtaagaagat cgacagcgaa ataatattcg
     9721 aatagtctta tctttttatt ttttgagatt tgactcagca gtgctctttc aatttctttt
     9781 cctgtgagat cttttgcatg aatgatcctg ttcttagaat gaccgccttc ttttccaagt
     9841 aaaagtgttc ctttatcatt ggaaaatttt gctccaaact ctgtaagttc tctgatcata
     9901 tcaggtcctt cttcaacaat ctttctgaca gcttttatat cacatagacc agctccgcag
     9961 tccagtgtgt catttatatg aagttcgaaa ttatcatcgt cactcatcac agcagcaatg
    10021 ccgccctgag cataatttgt attcgattcg gatttttgct ttttggtaat gactattact
    10081 ttcccaaaag cacttagctt caatgctagc gaaagacctg caataccgct gccgataatt
    10141 aaaaaatctg ttgtcaaaaa ttattgtttt atgaaattta aatatttcaa aaattaaaag
    10201 agcggacata atatccgctc agtaaaaata aatattaatc cgatgaaaat ttagattatt
    10261 acaggcttta aaatataaat gatgtttaaa aaaaaatcac atcaaatctt taattctt
//
LOCUS       DAORVQ010000112        10267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3392_length_10267_cov_5.698198, whole genome shotgun sequence.
ACCESSION   DAORVQ010000112 DAORVQ010000000
VERSION     DAORVQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_10267_cov_5.698198"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1776)
                     /gene="groL"
                     /locus_tag="PLX80_09620"
     CDS             complement(130..1776)
                     /gene="groL"
                     /locus_tag="PLX80_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500160.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQY21087.1"
                     /translation="MSKIISFDSEARSALKKGVDTLANAVKVTLGPKGRNVVIDKKFG
                     APTITKDGVTVAKEIELEDPIENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFREG
                     LRHVTSGANPMDLKRGIDKAVEELVKGLKEISSPIKENKEIAQVGSISANNDPYIGGL
                     ISEAMDKVGKDGVITVEEAKGTETTVDVVEGMQFDRGYLSPYFVTDAESMETELEDPY
                     ILIHDKKISTMKDLLPILEKTAQSGKSMLIIAEDIEGEALATLVVNKLRGTLKICAVK
                     APGFGDRRKAMLEDIAILTKATVISEEQGYKLENATVSYLGTAKRVVIDKDNTTIVEG
                     AGETDDIKKRVNEIKSQIDKTSSDYDKEKLQERLAKLSGGVAVLKIGASTEIEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALLRVGNRLDNLKGDNHDITTGIAIVKRAIEEPI
                     RQIVNNAGLEGSVVINKVKEGKDNFGFNAATEEYEDLVKSGVIDPTKVTRIALENAAS
                     VAGLLLMTEATIVDRPEKDKSPMMPPGGMGGMGGGMGDMY"
     gene            complement(1848..2132)
                     /gene="groES"
                     /locus_tag="PLX80_09625"
     CDS             complement(1848..2132)
                     /gene="groES"
                     /locus_tag="PLX80_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQY21088.1"
                     /translation="MNIKPLSDRIIVKPADAEEKTASGLIIPDTAKEKPMKGEIVAVG
                     KGKITDDGKEVKMELKVGDKVLYGKYSGTEITIEGDEFLIMRESDVYAII"
     gene            2431..4899
                     /locus_tag="PLX80_09630"
     CDS             2431..4899
                     /locus_tag="PLX80_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HQY21089.1"
                     /translation="MLKVKNKNITVTCFHCGELCRQSEITSEGKNFCCKGCKTVYEIL
                     KGNDLCSYYNQNEKTGQRLSDIKFENKFEFLNDNDIQEKILDFRDNKISKITLIVPGI
                     HCSSCIWLLEKLYKLNPAIKSSLVDFLNKKVTIIYDHNKISLKEIAVIMSSIGYEPNI
                     SLDKNNVQHKKNQFKTNKLYLKIGIAGFCLGNIMLLSLPEYFADRFNVDAELKNLFNY
                     LNILLSLPVFFYSASDYFISAWKAVKKKYINIDFPISLGLTALFVRSLYEIIIFNNAG
                     YFDSLAGLIFLLLIGKLFQSKTYESLNFERDYRSYFPVSVTLKNSINGDYKEVSIPVA
                     KLETGDRIIIRNGEIIPADSILFSGNANIDYSFVTGESMPEQKVLGEIIYAGGRHFGS
                     VIELEVIKTVSQSYLTQLWNNDVFKKDTLSGIQNLTDKFSKYFTIIILAIAFGAGIFW
                     LREDINKAIDSFTTVLIIACPCAFALTTPFAFGYTMRIFGRNKFYLKKGSVIEDLANT
                     NTVVFDKTGTITDTGNVSVKFFGSKLSHTELSLVKALVRNSSHPLSRKIFESIENDDY
                     SDELDVTDFMESISNGISGIVCGIPVKVGNIKYILKDLDCSYYDKSFEHKRNIFSTNV
                     FISVNNYIKGYYSFGNIYREGIDRVVGDLKVDYEMAVITGDNDSEKNNLLKIFSNNVK
                     LEFDQKPQDKLDFIKKLQENSANVLMIGDGLNDAGALKQSEVGISVSEDIANFSPSCD
                     AIIDADEFSKIGMFIRFSKTCMNIIRASFVISFIYNVIGLYLAASGVFSPLVAAVLMP
                     FNSISIIVIIFLLTNFFSKRMGLA"
     gene            4896..5111
                     /gene="ccoS"
                     /locus_tag="PLX80_09635"
     CDS             4896..5111
                     /gene="ccoS"
                     /locus_tag="PLX80_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQY21090.1"
                     /translation="MSAIIVLIGVSLTVAVIFLIAFIWSVKTGQYEDKYTPSVRMLFD
                     DNEKPYDEKNEKNNKKIIKHKNKNGDL"
     gene            5112..7235
                     /gene="ccoN"
                     /locus_tag="PLX80_09640"
     CDS             5112..7235
                     /gene="ccoN"
                     /locus_tag="PLX80_09640"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HQY21091.1"
                     /translation="MEMEKFRYDNKIVRDFAVATIVWGVVGMLVGLIASLQLITPSMN
                     MGNQFTTFGRIRPLHTNAVIFAFVGNAIFMGIYYSLQRLCKARMFSDLLSKIHFWGWQ
                     TIIVSAVVTLPLGFTTSKEYAELEWPIDILITLIWVVFAINMFGTIVKRREKHLYVAI
                     WFYIATVVTVAVLHIVNSIEIPVTLLKSYPVYAGVQDALVQWWYGHNAVAFFLTTPFL
                     GLMYYFLPKAANRPIYSYRLSILHFWSLIFIYIWAGPHHLLYTSLPDWAQSLGVAFSI
                     MLIAPSWGGMVNGLLTLRGAWDTVRDSAILKFMVVAVTAYGMATLEGPLLSLKNVNAV
                     AHFTDWIVAHVHIGALGWNGFLTFGILYWLIPKIYKTDLFSEKLANFHFWIGTLGIVF
                     YAAPLYWAGVTQSLMWKQFTPEGLLQYPNFLETVIKIIPMYIMRSIGGTLYLSGVLVM
                     VYNLFKTAKSGKLEANEDAEAAPLMKNSPETSKYKHRWIETRPGKFAILATIVILIGG
                     AVQMIPTFLIESNIPTITSVKPYTALELQGRDIYIREGCNNCHSQMIRPFRSETERYG
                     EYSKAGEFVYDHPFLWGSKRTGPDLQRIGKKYPNLWHYYHMNDPESISPGSIMPSYPW
                     LLEQELDISTTEAKINAMRKIGVPYSPEFGAISNTQLLDQASEIALDLKNNGVNVDNT
                     KEIIALIAYLQRLGTDIKVKDTAGN"
     gene            7265..7441
                     /locus_tag="PLX80_09645"
     CDS             7265..7441
                     /locus_tag="PLX80_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HQY21092.1"
                     /translation="MFKHYFEIIEGIDIYPIFSLLVFLIFFSMTIVFILKSDKEYIKE
                     MSNLPLEGDDQNEK"
     gene            7461..8450
                     /locus_tag="PLX80_09650"
     CDS             7461..8450
                     /locus_tag="PLX80_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQY21093.1"
                     /translation="MKFVKNAIKILTAGTLAIFLSVYEALAQTAPAAAGEPDMHLYTV
                     ILMVTILIILHAVFVPLLYPEKTPEEIAQSKNEVKVKSIVTILADKFSGLKPIEEEQD
                     LLMSDNYDGIRELDNNIPPWFNILFYGSMIFAVIYMLNFHVLKTGKLPLEEYSDEVYA
                     AELKRNELIRTGAFINEESVELDNDPESIVMGKQIFVANCVPCHGNNAEGTVGPNLTD
                     EFWIHGGGIKNVFKTVKYGVPVKGMIAWQNILNPKSIQQVSSYLISLKGTAPAFGKAP
                     EGEKYIDQNGSADSTSVKDDSLKTSGQTTKVKPDSVKNSINKKDTTKSDTLRK"
     gene            8452..9876
                     /gene="ccoG"
                     /locus_tag="PLX80_09655"
     CDS             8452..9876
                     /gene="ccoG"
                     /locus_tag="PLX80_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQY21094.1"
                     /translation="MSEINTDTKKKLIPESSDSFKDTMSIVNKDGGRKWVYPKKPKGR
                     FYNARTILSVFLLAFLFGIPWIKIDGHPFVLLNIIERKFILFGTVFVPQDFYLFGLLM
                     ISVFVILFLVTAVYGRIFCGWLCPQTIFLEMVFRKIEYFIEGDYIQQKNLNSSPWTES
                     KILKKFSKYTVFLIISFLIANTFLAYIIGTDQLLKIISEPVSQHLSGFLSIVGFTIFF
                     YWIFAWFREQACILVCPYGRLQSVLLDKNSVVIAYDNVRGELRGKVKEKVKDDLGDCI
                     DCKLCVNVCPTGIDIRNGTQLECVNCTACIDACDSIMDKIERPRGLIRFDSIENISTG
                     SGFKWTSRLTGYTVVLVILLTVVSVLFALRKDVDVNVLRTPGMLFQNQESNKVSNMYN
                     IKISNKTFDEKKIDFRLKDDIGEIKLIGNDAMIKPLEIHEGSMLVILPKEKIKFTNTP
                     VNIEVYSDGKKIDEINTNFLGPQN"
     gene            9888..>10267
                     /locus_tag="PLX80_09660"
     CDS             9888..>10267
                     /locus_tag="PLX80_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQY21095.1"
                     /translation="MNWGLKIILSFVIFATGITVIVVMSMSANVDLVHEKYYEQEINY
                     QKQIDILERSKSLKSDLSVNDEEGYILLTINNYQQYSGLYGKINFYRTADAERDYFVD
                     LKIDESGKQLIPKNDMINGAWKIK"
BASE COUNT         3294 a         1692 c         1887 g         3394 t
ORIGIN      
        1 acatagacct caaaaaaaaa agaccttctt aaaatttaag aaggtcttta aatatattat
       61 tggatttcaa tttcacatat cgaatattca acaattaaaa ctaactgaac attgacaatt
      121 gatcattgac tagtacatgt cacccatgcc gccgcccatg ccgcccatac cgccaggagg
      181 catcattggt gatttgtctt tttccggtct gtcaacaatt gtagcttcag tcataagaag
      241 aagacctgcc acggaagctg cgttctcaag tgctattctt gttactttag ttggatctat
      301 aacaccagat tttacaagat cttcgtactc ttcagtcgct gcattgaatc cgaagttgtc
      361 ttttccttct tttactttat tgatgaccac tgaaccttca agaccagcat tgttaacaat
      421 ttgtctgatc ggttcttcga tagctctttt tacaatagct atgcctgttg taatatcatg
      481 attgtcacct tttaaattat caagtctgtt accgactctt aaaagtgcaa caccgcctcc
      541 ggggacgata ccttcctcga ctgctgctct tgttgcatga agcgcatctt cgactctggc
      601 ttttttctct ttcatctcga tttcagtaga agcaccgatc ttaagtacag caacaccgcc
      661 tgataactta gctagtcttt cctgtaattt ctctttatca taatctgatg aagtcttatc
      721 gatctgtgat tttatttcgt taactctttt cttgatatcg tcagtctctc ctgcaccttc
      781 tacgatagtt gtattatctt tgtcgatcac aactctctta gcagttccaa gatatgaaac
      841 tgtagcattc tcaagcttat atccctgctc ttcagatatt acagtggctt tagttaggat
      901 cgcaatatct tcaagcattg cttttcttct gtcaccaaat ccgggagctt taactgcaca
      961 gatctttaga gtacctctta atttatttac aactaaagtt gcaagagctt caccttctat
     1021 atcttctgca atgatcagca tactttttcc tgactgagct gttttctcaa gaataggaag
     1081 aagatctttc atagtagaaa ttttcttatc atggatcagg atgtaaggat cttccagttc
     1141 agtttccatt gattctgcat ctgtcacaaa gtatggtgaa agataacctc tgtcaaactg
     1201 cataccttct acaacatcaa ctgtagtctc agtaccttta gcttcttcga ctgtgatgac
     1261 accgtctttt ccgactttgt ccatagcttc agaaataagt ccgccgatgt atgggtcgtt
     1321 attagcagag atagaaccaa cctgtgcaat ttccttattt tcttttattg gggatgaaat
     1381 ttctttcagt ccttttacta attcctcaac agctttatct attcctcttt tgagatccat
     1441 tgggttagct ccgcttgtta catgtctcag accttctctg aaaattgcct gagcaagaac
     1501 tgttgcagtg gtagtaccgt caccggctac atcggaagtt tttgaagcca cttctttcac
     1561 catctgagca cccatatttt caatcggatc ttcaagttcg atctctttag ctacagtaac
     1621 accgtcttta gttatagtcg gcgcaccgaa ttttttatct attacaacat ttctaccttt
     1681 aggtccgagt gtgactttca ctgcatttgc aagtgtgtca acacctttct ttaatgcgga
     1741 tcttgcttcc gaatcaaagc tgattatttt agacatattc tttttgtttt agtttttaaa
     1801 aattatttat ataaattttc acttgaaaga tttcaagcat cggtgattta gataattgcg
     1861 taaacatctg actctctcat gatcagaaat tcatcacctt ctattgttat ttctgtacct
     1921 gaatattttc cataaaggac tttgtctccg acctttaatt ccattttaac ttctttaccg
     1981 tcatcggtaa tttttccttt tcctacagct acgatctcgc ctttcattgg cttctctttt
     2041 gctgtatcag gaatgatcag acctgaagca gttttttctt ctgcatcagc cggttttacg
     2101 ataattctat cgcttaatgg tttaatgttc attttttacc tttcttaagt tgtttaatta
     2161 aaaaacactt atgttttaaa ttgatgttgt tagcactcta cgtgtaagac tgctaatata
     2221 ataatgttaa tatgggaatt caagtcatag atatagtctt tttaaaaaat tgaaaaatta
     2281 cgcacaatta ttctggtatc cttttaatga aaattttcta atttcattcg ggtcagtaca
     2341 gaaatttgta tctaatttaa attattttct gctttatgat ttaaatcata caatacttta
     2401 cataaagact ctattttacg gtcaaataaa atgctgaaag taaaaaataa aaatattaca
     2461 gttacttgct ttcactgcgg agagttatgc cgtcagtctg agattacttc cgaaggaaaa
     2521 aatttctgct gtaaaggctg taaaacagtt tatgaaatac tcaaaggcaa tgatctttgt
     2581 tcatactata accagaatga aaagaccgga caaagacttt cagatataaa atttgaaaat
     2641 aagtttgaat ttttaaatga caacgacatt caggaaaaaa ttcttgattt cagagataat
     2701 aaaatttcga aaattacatt aattgttccg ggtattcact gcagctcatg catctggctt
     2761 ttagaaaaat tgtataaatt aaatcccgct ataaaatctt ccttagtaga tttcttaaat
     2821 aagaaagtta cgattattta tgatcataat aaaatttctc taaaagagat tgctgtcatt
     2881 atgtcctcaa ttggatatga gccaaatatt tctcttgata aaaataatgt gcagcataaa
     2941 aagaatcagt ttaagacaaa taaactttat cttaagattg gtatagccgg tttttgtcta
     3001 gggaacataa tgctgctcag cttacctgaa tattttgcag acagatttaa tgtagatgca
     3061 gagcttaaga atctttttaa ttatctgaac atacttcttt cacttcctgt ttttttctat
     3121 tcagcttcag actattttat ttcagcatgg aaagctgtta agaaaaaata tatcaatata
     3181 gattttccaa tttcactcgg actaacagca ttatttgtca gaagtcttta tgaaataatt
     3241 atttttaata atgcaggcta ctttgattcg ctggcaggtt tgatatttct gctgttgata
     3301 ggaaaattat ttcagagtaa aacttatgaa tcgctaaatt ttgaaagaga ttacagatca
     3361 tatttccctg tgtcagtaac attaaaaaat agcatcaatg gagattataa ggaagtcagc
     3421 atcccggttg ctaagcttga aacaggagac aggatcataa tcaggaatgg tgaaataata
     3481 ccggcggatt caattctttt cagcgggaat gcaaatattg attacagttt tgtcacaggt
     3541 gaatcaatgc ccgagcaaaa agttcttgga gaaataattt atgcgggcgg aaggcatttc
     3601 ggaagtgtga tcgagcttga agtgatcaaa acagtttctc aaagttatct tactcagctt
     3661 tggaataatg atgtttttaa aaaagataca ttatcgggaa tacaaaattt aacagataag
     3721 ttcagtaaat atttcacaat aataatttta gcaattgcat ttggtgcagg aatattctgg
     3781 cttcgtgagg acattaataa agctatagat tcatttacaa ctgttctgat aattgcttgt
     3841 ccatgtgcat ttgcccttac cactccattt gcattcggat atacaatgag aattttcgga
     3901 aggaataaat tttatctgaa aaaaggaagt gtcattgaag atcttgcaaa tacaaatact
     3961 gttgtctttg ataagaccgg tactattact gatacaggta atgtttcagt aaaatttttt
     4021 ggaagtaaat tatcgcatac agaattaagt cttgtaaaag ctcttgtaag aaattcctct
     4081 catcctctca gcagaaaaat atttgagtcg attgaaaatg atgactactc ggatgagctt
     4141 gatgtgactg atttcatgga atccatcagc aacgggattt cagggattgt atgcggcata
     4201 cctgttaaag tcggaaatat aaaatatata cttaaagatc ttgactgttc atattatgat
     4261 aaaagttttg agcataaaag aaatatattt tcgacaaatg tatttatttc tgtcaacaat
     4321 tatataaaag gatattattc atttggaaat atatatcggg aaggaattga cagagtagtc
     4381 ggtgatctca aagttgatta tgaaatggct gtcattaccg gagataatga ttccgagaaa
     4441 aataatctgc ttaagatatt tagtaataat gttaagcttg agtttgatca aaagccgcag
     4501 gataaattag attttataaa aaaacttcag gagaattcag caaatgtcct gatgataggt
     4561 gatggtctga atgatgcagg agcattaaaa caaagtgaag ttggaatttc cgtttcagag
     4621 gacattgcaa atttttcacc gtcatgtgat gcaataatcg atgcagacga attttcaaag
     4681 atcggaatgt ttatcagatt ttcaaagacc tgtatgaata ttatcagggc aagttttgta
     4741 atatctttta tttacaatgt aataggtcta tacctcgctg catcaggagt tttctctccg
     4801 ttagtcgcag cagtgctcat gccgtttaat tcaatttcaa ttatcgtgat cattttctta
     4861 ttgacaaatt tcttttcaaa aagaatgggg ttagcttgag cgctataatt gttttgatcg
     4921 gtgtaagtct tactgtagct gtcatttttc ttattgcatt tatctggtca gtaaagacgg
     4981 ggcagtatga agataaatat acaccatctg tcagaatgct ttttgatgat aatgagaaac
     5041 cttatgatga aaaaaatgaa aaaaataata agaaaataat taagcataaa aataaaaacg
     5101 gagatttata aatggagatg gaaaaattca ggtacgataa taagatcgta cgtgattttg
     5161 cagtagccac aatagtctgg ggagtcgtag gaatgctggt aggtctgata gcatctttgc
     5221 agttgattac accttcgatg aacatgggaa atcagttcac gactttcgga aggatcagac
     5281 ctttgcatac aaatgcagtt atctttgcat ttgtcggcaa tgcaatcttt atgggaattt
     5341 attattccct gcaaagactc tgcaaagcaa gaatgttcag cgatctgctg agtaagattc
     5401 acttctgggg ctggcagact attatagtat cagctgtagt cacgcttcca cttggattta
     5461 caacaagtaa agaatatgcc gaactcgaat ggccgatcga tattctgatc acgttgatat
     5521 gggtagtgtt tgcaataaac atgttcggaa caattgtaaa gagaagggaa aaacatttgt
     5581 atgtagcgat ctggttttat atcgcaacgg tagttacagt agcagttctt cacattgtaa
     5641 actcaatcga gatccctgtt actttgctga aaagttaccc tgtctatgca ggagttcagg
     5701 atgcactggt tcagtggtgg tacgggcata acgcagttgc attttttctt actactcctt
     5761 tccttggact aatgtattat tttttaccaa aggcagcgaa cagacctatc tattcttaca
     5821 gactttcaat actgcacttt tggtcgctga tttttattta tatctgggca ggtccccatc
     5881 atttgctata tacttcactt cctgactggg ctcagtccct gggagttgca ttttccatta
     5941 tgctgatcgc tccttcatgg ggcggtatgg tgaatggact gcttacatta agaggtgcgt
     6001 gggatactgt gcgtgacagt gcaattttaa aatttatggt agtagctgta actgcatatg
     6061 gaatggcaac acttgaagga cctttgctgt cattgaaaaa tgtaaatgcc gtagcgcatt
     6121 tcactgactg gatcgttgca cacgttcaca tcggagctct tggatggaat ggatttttaa
     6181 cattcggaat attgtactgg ctgatcccaa aaatttataa gacagattta ttttccgaaa
     6241 aacttgcgaa cttccacttc tggatcggaa ctctcggaat agttttttat gcagcaccgc
     6301 tttactgggc aggtgtaacg cagtcactca tgtggaaaca atttacacct gaaggattat
     6361 tacagtatcc gaattttctg gaaactgtga tcaagataat tcctatgtac ataatgagat
     6421 ccatcggcgg tactctttat cttagtggag ttttggtaat ggtttataat ttatttaaaa
     6481 ctgcaaagtc aggaaaactt gaagccaatg aagatgctga agcagctcct ctgatgaaaa
     6541 attcaccgga gacttccaaa tataaacaca gatggatcga gactcgcccg ggtaaatttg
     6601 caatacttgc aactatagta atactgatcg gaggtgctgt tcaaatgatt ccaacttttt
     6661 taatcgaatc aaatattcca actataacta gtgtaaaacc ttacactgct cttgaattac
     6721 agggaagaga tatttacata agggaaggtt gtaataattg tcattcacag atgatccgac
     6781 ctttcagatc tgaaacggaa aggtatggag aatattccaa ggcaggggaa ttcgtttatg
     6841 atcatccgtt tctatgggga tcgaagagaa caggtcctga cctacagcgg ataggaaaaa
     6901 aatatcctaa cctatggcat tattatcaca tgaatgatcc tgaatccatt tcacccggat
     6961 cgattatgcc gtcttatcca tggctgctcg agcaggagct ggatatttct actaccgagg
     7021 caaaaataaa tgcaatgaga aaaattggtg tcccttatag tcctgagttt ggtgcaatat
     7081 caaatacaca gctgttagat caggcttcag aaattgcact cgatctgaaa aataacggag
     7141 taaatgtaga taacacaaaa gaaattattg cactgatagc ttatctgcag aggcttggca
     7201 ccgacataaa agttaaagat acggcaggaa attaattaat aattaaataa aattttataa
     7261 taaaatgttt aaacattatt ttgaaataat agaaggaatt gatatctatc caatattttc
     7321 acttcttgta tttctgatat tcttttcaat gacaattgtt tttattttaa aatcagataa
     7381 agaatacata aaagaaatga gcaatcttcc actggaagga gatgaccaaa atgaaaaatg
     7441 atttatttag gaataataaa atgaagtttg taaaaaatgc tataaaaatt ttaactgccg
     7501 gaacattagc tatattttta tctgtgtatg aagctcttgc tcagactgca ccagcagcag
     7561 ctggtgaacc tgacatgcat ttatatactg ttattttaat ggtcacaatt ctgatcattc
     7621 tgcatgcagt atttgttccg ttactttatc ctgaaaaaac acctgaggaa attgcgcagt
     7681 caaaaaatga agtaaaagta aaatccatag ttactatact tgccgataag ttctccggtc
     7741 tgaaacctat cgaagaagaa caggatctgc tgatgagtga taattatgat ggaattagag
     7801 agctggataa taatattccg ccttggttta atattctctt ttatgggtct atgatatttg
     7861 ctgtaattta tatgttaaac tttcatgtat taaaaaccgg taaacttccg cttgaagaat
     7921 attcggatga agtatatgct gctgaattaa aacggaatga acttatccgg acaggggctt
     7981 tcataaatga agaatctgtc gagcttgata atgatcccga atctatagta atgggtaagc
     8041 agatttttgt tgcgaactgt gttccctgtc atggtaataa tgctgaagga acagtaggtc
     8101 caaatctaac agacgaattc tggatccacg gcggaggtat caaaaatgtt tttaaaactg
     8161 tgaaatatgg tgtacctgta aaaggaatga tcgcatggca gaatattctt aatccaaaat
     8221 ctattcagca ggtatccagt tatttgattt cccttaaagg tactgcacct gcattcggca
     8281 aagctcctga aggtgaaaag tatatagatc agaatggatc agctgattca acttcagtaa
     8341 aagatgattc acttaaaaca tcaggacaaa cgacaaaagt gaaaccggat tctgtgaaaa
     8401 attctatcaa taaaaaagat actactaaat cagatacatt aagaaaataa aatgtccgaa
     8461 ataaatactg atacgaagaa aaaattaata ccggaaagta gtgattcatt taaagatact
     8521 atgtctattg tcaataaaga tggaggcagg aaatgggtct atccaaagaa accaaaaggt
     8581 agattctata atgcacggac tatacttagc gtctttctgc tggcatttct ttttggaata
     8641 ccatggataa agattgacgg ccatccattt gttcttttaa atattattga gagaaaattt
     8701 attttatttg gaacagtatt cgttcctcag gatttttatt tgttcggatt attaatgatc
     8761 tctgtttttg taattttgtt tttggtcact gctgtgtatg gaaggatctt ctgcggatgg
     8821 ctttgtcctc agactatttt tcttgagatg gtctttagga aaattgaata ttttatagaa
     8881 ggtgattaca tacagcaaaa aaatctgaat tcttcgccat ggacggaatc aaaaatttta
     8941 aaaaaatttt caaagtatac ggtattttta attatctctt ttctgatagc aaatactttt
     9001 cttgcatata ttattggaac ggatcaactt ttaaaaataa tcagcgagcc tgttagtcag
     9061 catctcagcg gatttttatc tattgtagga tttacaatat ttttctattg gatattcgct
     9121 tggttccgtg aacaggcatg tattctcgtc tgcccgtatg gaagacttca gagcgtgttg
     9181 cttgataaaa attcagtggt cattgcttat gacaatgtaa gaggggagct tagagggaaa
     9241 gtaaaagaaa aagtaaagga tgatcttggt gattgtatag actgcaaatt atgtgtaaat
     9301 gtctgtccta ctggaattga tatcagaaac ggaacgcagc tcgaatgtgt aaactgtact
     9361 gcctgcatag atgcatgtga ttcgatcatg gataagattg aaagaccaag aggactgatc
     9421 agatttgatt ctatcgaaaa tatttcgact ggctcaggat ttaaatggac atcgagactg
     9481 acaggatata cggtagtatt ggtaatactt ttgactgttg tatcggtctt gtttgcttta
     9541 agaaaagatg tagatgtaaa tgtattaagg actccgggta tgctttttca gaatcaggag
     9601 agtaacaaag taagtaacat gtataacatt aaaatttcga ataagacctt tgatgaaaaa
     9661 aaaattgatt ttagattgaa agatgatatt ggagaaataa aattgattgg taatgatgcc
     9721 atgatcaaac cgcttgagat tcatgaagga agtatgctgg taattttacc aaaggaaaaa
     9781 ataaagttca cgaatactcc tgtaaacatt gaagtatatt ctgacggaaa aaaaatagat
     9841 gaaataaata cgaactttct cggaccacaa aattaattat aaataaaatg aactggggac
     9901 taaaaataat tttatctttt gtaatttttg caacaggaat tacagtaatt gtagtaatgt
     9961 caatgtctgc taatgtggat cttgtgcatg aaaaatatta tgaacaggaa ataaattatc
    10021 agaaacagat tgatatactt gaaagatcaa agagtttgaa atcggatcta tctgtaaacg
    10081 atgaagaagg gtatatttta ttaacgatta ataactatca gcaatattcc ggcctatacg
    10141 ggaagataaa cttctacaga acggcagatg cagaaagaga ttattttgta gatctgaaaa
    10201 ttgatgaaag cggcaaacag cttattccta aaaatgatat gattaatgga gcatggaaaa
    10261 ttaaatt
//
LOCUS       DAORVQ010000113        10167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3457_length_10167_cov_5.136175, whole genome shotgun sequence.
ACCESSION   DAORVQ010000113 DAORVQ010000000
VERSION     DAORVQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3457_length_10167_cov_5.136175"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..915)
                     /locus_tag="PLX80_09665"
     CDS             complement(217..915)
                     /locus_tag="PLX80_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21096.1"
                     /translation="MKLKLSIEDPCNENWDEMKEEFNSRFCSKCSKNVKDFTQMKEEE
                     IHRYLKENKKDKICGRFYSEQLGIIEYNEYKENFNTHKPSVIKNLTFFSAVALTSAAI
                     SLSSCNNEHSHTEKIKLQDSNNVNRDLDIQNRKLIDISDNKNNLQKEKKEKYDLENPN
                     KDMLDRADSIAGADNFKKNKDKKPKINDTTIIVKGGLVMGGIRTNEVKGESTVLGKLK
                     LQEEDDIIHPSKFK"
     gene            complement(1024..2523)
                     /locus_tag="PLX80_09670"
     CDS             complement(1024..2523)
                     /locus_tag="PLX80_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21097.1"
                     /translation="MLKSKAIDIIKTFSPEELKRFVSFVSSPYFNNVKSLIRLSEELR
                     NYYPDFSSEDLTEVSLYEKVYGKGKFSYSVMKNLMSDLIPLCEKFLVHDRLRKAVSEK
                     DGNSIELMQELRERNLNNHFNSRSKKFSELLRGHKYDNIEHFIKSNQHEILKYQYHLQ
                     KHKHSKLIWKGFLKRSDFDMCSIFHTLYLTSAVLIHSADHFKGNEEESLLVKFVRELD
                     LGSFLKTFDPNDSEEYFFINLYSKLTLLTLEENEDHSEDYFYDIKSSVINNVKLFSQV
                     DIYDILKSLRSYCISRMRMMKSKFIDELFEIDKLLVEKVNYNSAYIKWYIGELFTEIV
                     QLCIYSKNYEYAESFIEKFKPLLSTDIVEFETGFTKAFLYLEYGETDKALELLSKIKP
                     INNSMKLLIKNLYLRAYYEKGYFEEGFSMLDAFIHFINKEENLTGVRSKLYNLKYNVY
                     LKLYKMRVSPEKYSGHDINNLRKELDKFYILADKDWYLNKLKDLEDLIE"
     gene            complement(2586..4046)
                     /locus_tag="PLX80_09675"
     CDS             complement(2586..4046)
                     /locus_tag="PLX80_09675"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HQY21098.1"
                     /translation="MISFENLLLIISVLIVLSILIIKVSENFGLPALLLFLGVGMLAG
                     SDGIGGIYFEDVSVTQSVGMLALIFILFSGGLDTNIKNVFPVRNEAFSLATLGVLATT
                     FFTGLFCYYVLGFELIYGLLIGAIISSTDAAAVFSILRTNKIGLKNNLKPLLEFESGC
                     NDPMAIFLTMTITGIIVSNEFNAVSVSLDLFRQFAFGGILGYAAAKGLVKLFNKINLS
                     NEALYPVLLLSSAVLIYSFTVFTGGSGYLAVYVAGIIIGNSNIIQKKSLLRFFDGLAW
                     LGQIGMFLTLGLLVNPSELLLIMGTGILLSFFLILLGRPMSVFISLMFSKYKYNEKAA
                     VSWIGLRGAVPVILATIPLSAGLDDNHIIFNIVFFVVLSSVLIQGWTIKYIADFFKVT
                     EKSDIVSISPIQFETSKNLDSELIDFIVPFNSPISGQTIIELGLPKDCLIIFICRDEI
                     FFVPEGNTFVAEGDILQILVSKKEIKNVIKKLSKTV"
     gene            complement(4461..6782)
                     /locus_tag="PLX80_09680"
     CDS             complement(4461..6782)
                     /locus_tag="PLX80_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY21099.1"
                     /translation="MTIKFNIKYTVILSFLFLMIFVSGSFSQSTGVVSGKVIDITNQQ
                     PVSDVSVSVLKDKQVIGGSVTDDKGFFVITEIPVGEYSLRFSLTGYFTYFIDNIVVNS
                     GAPADVLAEIETVTTEEIEVTEERFRNPSDISNSFKNLTYEDIRRAPGGFEDIGRVVQ
                     TLPGVAFVNDGRNDLIVRGGSPSENLFLVDNAVIQNINHFGSQGATGGPVSIINLELV
                     RDVSFVTGGFPAKFGDKLSSVLELKLREGNRDRFLSNINLSATGFGAVFEGPLGNDKQ
                     GSWIFSARRSYLDFIFNASGFGFVPAYSSLQLKGVYDFNNKNSLTVNVFGNLDNVTFN
                     NDTEENIQDNETILDNDQKGYVNTYEFKTLLSKKSFALFNLGRTFNNFSYEGRDSSFN
                     QIFTNNSKEGETTLKAEYFLTPEAETQLEFGAAWRFVDFENQIFQDVDTTYYISETTN
                     ERYVLPALDINDDNKTNKAYAFAQVTQTLFGKVKLNAGLRYDYFGFINSKNYISPRAS
                     VIYPFSEKLNMSFAYGTYYQSPSYVWLIANPANRNLDDIRADHYIAGLEYLFEADFRV
                     TVEAYYKNYSDYPVSTLRPYIILANTGGDFEQTDDFGLEPLVSAGTGYSKGIELFIQK
                     ALTNDFYGTLNLSLFEAKYTALDGVERTSDFDNRYIFTLTGGYKFAESWEAGAKLRLA
                     GGRPYTPINPNDGTRLVSEYNTARLPDYSRLDVRVDKNWNFKKWTLVTYIDIQNILNK
                     KNITAIDWNQYTNLIEENESIGILPSIGINVMF"
     gene            6904..7788
                     /locus_tag="PLX80_09685"
     CDS             6904..7788
                     /locus_tag="PLX80_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQY21100.1"
                     /translation="MSEYFISDLEKYTGIKAHTIRIWEQRYNILKPKRTLGNTRYYDE
                     NDLKRLLNISSMRKHGLKISEISKMGELEISRFIDEIIKQNNTGEELNEYFINELIVS
                     SLNFDQNKFEKIFSNSLIKFGIEKTYVSILLPFLNRLGLLWRQATVTPAQEHFVTELI
                     KMKLMSIIDTISLDSLGKKTWLLFLPEYENHEIGLLFSYYLLRIKKIKVIYLGANVPL
                     ESLKESVRLTSATDLYFFMVSNHDTNELKKYLKELSESFSKQSVHFSCKPDLIKGIKL
                     PKNCNCILKPEDLIIEGD"
     gene            7821..9290
                     /gene="crtI"
                     /locus_tag="PLX80_09690"
     CDS             7821..9290
                     /gene="crtI"
                     /locus_tag="PLX80_09690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HQY21101.1"
                     /translation="MKASVIGSGFSGLAAASYLGKEGIDVEIYEKNDDIGGRARSFKT
                     QNGYTFDMGPSWYWMPEIFENFFNNFGFSQSDFYKLKLLDPSFRMIFKDDFVIDVPSD
                     YEKLCELFESLETGSAGKLKKFMNEAEYKYNLGMNKLAQKPGNSLFEYFDFRFLKGLA
                     SMDVFSSFSSHIRKYFSHPAIISILEFPVLFLGAMPSKTPALYSLMNYAGLKLGTWYP
                     EGGFGNVINAFKKVTEKYSIPINLSSPVSSIIVNKNKARAVRVNGVDVNTDAIIASAD
                     YHHVESKLLEYEFRNYSESYWDKKTFAPSCLIYYVGITKKIKNLNHHNLFFQNDLNLH
                     AEEIYDSPKWPTQPLFYLCCPSKSDKTIAPEGHENLFLLMPIAPGLEDDTKIREKYFD
                     IMMDRIEKFTSEKIRQFIDYKKSYCISDFISDYNAYKGNAYGLANTLRQTAVLKPKIR
                     SKKINNLFFTGQLTVPGPGVPPAILSGKIAANELIKSFR"
     gene            9292..10128
                     /locus_tag="PLX80_09695"
     CDS             9292..10128
                     /locus_tag="PLX80_09695"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HQY21102.1"
                     /translation="MKTLFDNISVECSKLVTKTYSTSFSAGIRFLHKDLRNPVYNIYG
                     FVRFADEIVDSFHDFDKKELLDKFSDDTFEAIEKKISLNPILNSFQDTVRKYEIPNEL
                     IEQFLSSMRMDLNRVSYDSDELNDYILGSAEVVGLMCLKIFTYEDKSLYEKLKPFAMK
                     LGSAFQKINFLRDLKTDYNELSRSYFPNFNPDNFSDDDKRKIESDIENELITALEGIG
                     KLPVSSRKGVYLAYLYYLKLFKKIKRLPAKEIMKRRIRISNGVKAGLVINLFARKLAG
                     EV"
BASE COUNT         3442 a         1679 c         1502 g         3544 t
ORIGIN      
        1 cggaaatagt tgtgaatatg catttcctga aaaaaaggaa aatgttagaa ttataaatgc
       61 taaacgtaat ttcaaattat tatttgctta aagtttcagg attcatttct cgttttttgg
      121 agggtttaat ttaacaaaat tagatttgta ataaaaggat tttttaattg gtaattttgt
      181 aatcgctgaa ataaaaattg ttaattttaa ttaagtctat ttaaattttg agggatgaat
      241 tatatcatcc tcttcttgaa gtttcaattt tcccaataca gtactttctc ctttgacttc
      301 atttgttctt attcctccca ttactaagcc tccctttact atgatagttg tatcatttat
      361 ttttggtttc ttatctttat ttttcttaaa attatctgct cctgctatac tatcagctct
      421 atccaacata tccttgtttg gattttccag atcatatttt tctttctttt ccttttgtaa
      481 attattttta ttatcagaaa tatcaatcaa ttttctattc tgaatatcaa gatctctgtt
      541 tacattgtta ctatcctgta acttaatttt ttctgtgtgg ctatgttcat tattgcagga
      601 tgagagtgaa atagctgcgg aagttaaagc tactgcagaa aaaaaagtca gatttttaat
      661 aactgatggt ttatgagtat taaaattctc tttatattca ttatattcaa taattcctaa
      721 ttgttcggaa taaaatcgtc cgcaaatttt atctttttta ttttctttta aataccgatg
      781 gatctcttct tctttcattt gggtaaagtc tttaacattt ttactgcatt tactgcaaaa
      841 tctggaatta aattcttctt tcatttcatc ccagttttca ttacatggat cttcaatgct
      901 gagttttaat ttcatacttt ttcacattaa taatttaaaa gctaattaaa ctttatttat
      961 tagtgtttaa tcaacatcac tcagaagagt ttgttccctt taaatgtttt cagtttatta
     1021 ttattattca attaaatctt caagatcctt tagcttattc agataccagt ctttgtcagc
     1081 cagaatataa aacttatcaa gttcttttct cagattatta atgtcatgcc ctgaatattt
     1141 ttcaggcgat actctcattt tatataattt taaatataca ttatatttta gattataaag
     1201 tttgcttctg actcccgtta aattttcttc tttgttaatg aaatgtatga atgcatccag
     1261 catggaaaat ccctcttcga aatatccttt ttcataataa gctcttagat ataaattttt
     1321 tataagtaat ttcattgaat tattaattgg ttttatttta gataataatt caagtgcttt
     1381 atctgtttca ccatattcaa gatacaggaa agctttagta aatcccgttt caaactcaac
     1441 aatatctgta cttagcagtg gtttaaattt ttctataaac gattccgcat attcataatt
     1501 tttgctgtaa atacatagct gaactatttc tgtaaacaat tccccaatgt accatttaat
     1561 ataagctgaa ttgtagttta ctttttccac aagtaattta tcaatttcaa aaagttcatc
     1621 tataaattta cttttcatca ttctcattct agaaatacag tagcttctca gacttttaag
     1681 aatatcatat atatccacct gagaaaatag ttttacatta tttattacag aagattttat
     1741 atcataaaaa taatcttcac tgtggtcttc gttttcttcc agagtcagta gtgtaagttt
     1801 tgaatacaga tttataaaaa aatattcttc tgaatcattc ggatcaaaag tttttaaaaa
     1861 tgatccaaga tcaagctctc tcacaaattt tactaataag ctttcttctt cattaccttt
     1921 aaaatgatca gccgaatgaa tgagtactgc acttgttaag taaagagtat gaaaaatact
     1981 gcacatgtca aaatctgatc ttttgagaaa acctttccat attagtttac tgtgtttgtg
     2041 tttctgaaga tgatattgat attttagtat ctcgtgttga tttgatttta taaaatgctc
     2101 aatgttatca tatttatgtc ctctcagaag ttcactaaat tttttactcc tgctgttaaa
     2161 atgattattc agatttcttt ctcgcagctc ctgcattagt tcaatggaat tcccatcttt
     2221 ttctgaaaca gcttttctta atctgtcatg tacaagaaat ttctcacaaa gagggatcaa
     2281 gtccgacatc agatttttca ttacactgta actaaatttg cctttcccat ataccttttc
     2341 atataatgaa acttctgtca gatcctctga tgaaaaatca ggataataat ttcgtaattc
     2401 ttcactcaga cgtatcagac ttttgacatt gttaaagtaa ggtgaagaaa caaaacttac
     2461 gaacctcttt aattcttcag gagaaaaagt tttaattata tctatcgctt tgctttttaa
     2521 catgctcttt tactaataat attcaaattt catataaatt taaaaaccgc tttgacattt
     2581 tgtatttaaa cggttttcga aagtttttta atcacatttt ttatttcttt tttgcttact
     2641 aatatctgca atatatctcc ttcggcaaca aaagtatttc cctccggtac aaaaaatatc
     2701 tcatctctgc atataaaaat tatcagacaa tcttttggaa ggccgagctc aattatagtc
     2761 tgtcctgata tcggtgaatt aaacggtaca atgaaatcta taagttcaga atcaagattt
     2821 ttagaagttt caaattgaat tggtgatatt gaaacaatat ctgatttttc tgtaacttta
     2881 aaaaaatcag caatatattt tattgtccag ccctgaatta aaactgatga caatactaca
     2941 aaaaacacaa tattaaaaat aatatgatta tcatcaagac ctgcggataa aggaatagtc
     3001 gcgagtatca caggcacagc tcctcttaat ccgatccatg agacagcagc cttttcattg
     3061 tatttatatt ttgaaaacat caaagaaata aaaacactca ttggtctgcc gaggagaatt
     3121 aaaaaaaatg aaagcaaaat acctgttccc attatcagta aaagctcact tggatttaca
     3181 agcagcccta atgtcaggaa cattcctatt tgtccgagcc atgctaaccc gtcaaaaaat
     3241 ctcaacaaag attttttctg aatgatgttg ctgtttccga taataattcc ggcaacatat
     3301 acagcgagat atccgctgcc accggtaaat actgtaaaag aatatatcaa tactgctgaa
     3361 gacagaagaa gtactggata caaagcttca ttacttaaat ttatcttgtt gaataatttt
     3421 acaaggcctt tggcagctgc atatcctaag atcccaccga atgcaaattg tctaaaaaga
     3481 tctaaagaaa cactgactgc attgaattca tttgatacta ttatccctgt aatagtcatt
     3541 gtcagaaaga tagccattgg atcgttgcat cctgattcaa actccagtag aggtttaaga
     3601 ttattcttca gtcctatctt gtttgttcga agaatggaaa atacagcagc agcatctgta
     3661 gaggaaatta tagcacctat aagaagtcca tagattagtt caaatccaag tacatagtaa
     3721 cagaataatc ctgtaaaaaa agttgtagct aacacaccaa gcgtagcaag gctgaacgct
     3781 tcatttctga cgggaaatac atttttaata tttgtatcaa gtccgcctga gaataaaata
     3841 aagataagtg caagcatacc tactgactga gtcacactta catcttcaaa ataaatacct
     3901 ccaataccgt ctgagcctgc aagcattcca acaccaagaa acagcagcaa tgccggcagt
     3961 ccgaaatttt cagatacttt gattattaaa atgcttaata caataagcac agaaattatc
     4021 aataataaat tttcgaagga tatcaataat aagaattagt ttgaagataa gataaaaata
     4081 tttgctgtta attcaaatta aaagttttcg gtatttggga atggacacta tggtttataa
     4141 tatattcttt tctgttattt caaatatatt ataattaaat aaacagttgt aacaattttt
     4201 attggaataa aaatatgccc tttaatgaac tacctctgaa gatatgaata atttctttta
     4261 ggataagttg aaactaattt caataatata tgtaagttct aatagcaaaa aattattttt
     4321 agaaataatc cttttgctaa aatcattgta gttccaaaaa accaaccctc actttttaat
     4381 tttcaatttt aaatgttcaa cgttcaatgc tttaatttaa tgcaattcga taagaattcc
     4441 tttaattaat tttttgtatt ctaaaacatc acattaattc caattgatgg caggattccg
     4501 atactttcat tttcttctat taaatttgta tattgattcc agtcgattgc agttatgttt
     4561 ttcttattaa ggatattttg tatatctatg taagtcacca aagtccattt tttaaaattc
     4621 cagttcttat caactcttac atcgagtctg ctgtaatccg gcagtcttgc agtattatat
     4681 tcagatacta atcttgttcc gtcattcgga tttatcggcg tgtaaggtct tcctccggca
     4741 agtcttaact tcgctcctgc ctcccagctt tcagcaaatt tgtatcctcc tgttaaagtg
     4801 aaaatatatc tgttgtcaaa gtcactcgtc ctttcaacac cgtcaagtgc agtatatttt
     4861 gcttcaaaca aagacagatt cagcgttcca taaaagtcat ttgttaaagc tttctgaata
     4921 aataattcta ttccttttga atatcctgta cctgctgaca caagtggctc taacccaaaa
     4981 tcatcagttt gttcaaaatc tccgccggta tttgctagaa ttatatatgg acgtaaagta
     5041 ctgacaggat aatctgaata atttttataa taagcttcta ctgtaactct gaaatctgcc
     5101 tcgaataaat attccaatcc cgctatataa tgatcagcac gaatgtcgtc caaattcctg
     5161 ttcgcaggat ttgcgatcag ccagacatat gaaggagact gataatatgt tccatatgca
     5221 aaactcatat tcagtttttc agaaaacgga tagatcacag aagctctcgg cgaaatataa
     5281 tttttgctat ttatgaatcc aaagtagtca tacctcagac ctgcattcag cttaacctta
     5341 ccaaacaatg tctgagtaac ctgagcaaat gcataagcct tattggtttt attatcatca
     5401 ttaatatcaa gagcaggcag aacatatctt tcattcgtag tttcagaaat ataataagtt
     5461 gtatccacat cctgaaatat ctgattttca aaatcgacaa atctccatgc agctccaaac
     5521 tcaagctgcg tttcggcttc aggagttaaa aaatattcag cttttaaagt ggtctcacct
     5581 tcttttgaat tatttgtaaa tatctgattg aatgaggaat ctctgccttc ataactgaaa
     5641 ttattaaatg tccttccaag attaaataaa gcaaaagatt tttttgacaa aagagtttta
     5701 aactcataag tatttacata acccttttga tcattatcta aaatagtctc attgtcctga
     5761 atattttctt ccgtgtcatt attgaaagtt acattatcca gattaccgaa aacatttact
     5821 gtgagtgaat ttttattatt aaaatcataa acacctttca gctgcaatga agaataggcc
     5881 ggtacaaacc caaagcctga tgcattaaaa ataaaatcaa gataacttct tcttgctgaa
     5941 aagatccatg atccctgttt gtcatttcct aacggtcctt caaacaccgc tccgaaacct
     6001 gttgcactta gatttatatt gctcagaaat ctgtcacggt ttccttctct aagctttagc
     6061 tcaagcacag aagaaagctt gtcgccaaat tttgcgggaa agccaccggt tacaaagctc
     6121 acgtcacgga caagttcaag atttattata ctaaccggac cgcctgttgc tccctgtgat
     6181 ccgaaatgat ttatattctg tataactgca ttatccacca gaaaaagatt ttcactcggc
     6241 gaaccgcctc ttacgataag atcatttctg ccgtcattca caaaagcaac tccgggcaga
     6301 gtctgcacta ctcttccaat atcttcaaat ccgcctggtg cacgtctgat gtcttcataa
     6361 gttaaatttt taaatgagtt tgaaatgtca cttgggtttc tgaatctctc ttctgtaact
     6421 tcgatctctt cagttgtaac tgtttcaatc tctgccaaaa catccgctgg tgcaccgctg
     6481 tttacaacaa tgttgtctat aaaatatgta aaatatccgg tgagactaaa tcggagtgaa
     6541 tattcaccta cagggatttc cgttatcaca aaaaatcctt tatcatcagt tacagatccg
     6601 ccgattacct gcttatcctt taatactgaa accgatacat ctgaaaccgg ctgctgattt
     6661 gttatatcaa ttacctttcc ggaaactaca ccagtggact gtgaaaagct accggatacg
     6721 aatatcatta aaaaaaggaa agataaaatt actgtatatt taatattgaa tttgattgtc
     6781 ataaatttat ttattgtatg aagattttgt atatctggtt ttttcgattt ataaaataat
     6841 aatttatggt gttgtaatta ttattttgtt taagtaattt aaaataatct taaacaaata
     6901 tcaatgtctg aatatttcat ttctgatctg gaaaaataca caggaataaa agcgcataca
     6961 atcaggatct gggaacagcg ttataatata ctgaagccaa aacgaacact gggaaacaca
     7021 agatattatg atgaaaatga tctgaaacga ttactgaata tttcatcaat gagaaagcac
     7081 ggtttgaaaa tatcagaaat aagtaaaatg ggagaattgg aaatttccag atttatagat
     7141 gaaataataa agcagaataa tacaggcgaa gagttaaacg aatatttcat caatgaatta
     7201 attgtaagtt ctttaaattt cgatcagaat aaatttgaaa aaattttctc aaactcatta
     7261 atcaaatttg gaattgaaaa gacatatgtt tcaattttac ttcctttttt aaaccgttta
     7321 ggacttctat ggaggcaggc tacagtgact ccagctcagg agcactttgt gactgagctt
     7381 attaaaatga aattaatgtc aatcattgat acaatttcgc tggactcttt aggaaaaaaa
     7441 acctggttat tatttcttcc tgaatatgaa aatcatgaaa tcggtttgct tttttcgtac
     7501 tatctgctcc gtataaaaaa aatcaaagtt atttatttgg gagcaaatgt acctctcgaa
     7561 tcattaaaag aatcagtcag actaacaagt gccacagacc tttacttctt tatggtttca
     7621 aatcatgaca caaatgaact taagaaatat ttaaaagaat tatccgaatc tttcagtaag
     7681 cagagtgtcc acttttcatg taaaccggat ctaataaaag ggattaaact tcctaaaaac
     7741 tgtaattgta tattaaagcc ggaagattta ataattgaag gggattaatt gtttaagtat
     7801 tttcaaattt ttatcaacaa atgaaagcat cagttattgg ttcaggattt tcaggtcttg
     7861 cagcggcttc atatcttggc aaagaaggta tagatgtgga gatttatgaa aagaatgatg
     7921 atattggcgg cagagccaga agttttaaaa ctcagaatgg atatacattt gatatggggc
     7981 caagctggta ctggatgccg gagattttcg aaaatttctt taacaatttc ggattttccc
     8041 aaagtgattt ttataagcta aagctacttg atccttcatt tagaatgata ttcaaagatg
     8101 attttgtgat agatgttccg tcggactatg aaaagttatg tgaattattc gaaagtcttg
     8161 aaaccggaag tgcagggaaa ctgaaaaaat ttatgaatga agcagaatat aaatataatc
     8221 ttggaatgaa taagcttgct caaaagccgg gtaattctct gtttgaatat tttgatttca
     8281 gatttttaaa aggattagct tcaatggatg tgttctcaag ctttagttct catatcagga
     8341 aatatttcag tcacccagct atcatttcaa tactggaatt tccggttctg ttccttggtg
     8401 ctatgccatc gaaaacaccg gcactttaca gtctgatgaa ttatgccggt ctaaagcttg
     8461 gtacatggta tcctgaagga ggttttggaa atgttataaa tgcatttaaa aaagtaactg
     8521 aaaaatattc tattccaata aatttatctt cacctgtatc ttctattatt gttaataaga
     8581 ataaagcccg ggcggttaga gtaaatggtg ttgatgtaaa cacagatgca atcatagctt
     8641 cagcagatta tcatcatgta gaaagtaaat tgctcgaata tgaattcaga aattattctg
     8701 aatcttactg ggataaaaaa actttcgctc cgtcctgtct gatatattat gttggaatta
     8761 caaaaaaaat taaaaatcta aatcaccaca atttattttt tcaaaatgat ttgaatcttc
     8821 atgcagaaga aatttatgac agcccaaaat ggccgacaca acctctgttc tatttatgct
     8881 gtccttctaa atcagataag accattgccc ctgaaggtca tgaaaattta ttccttctta
     8941 tgcctattgc tcccggactt gaagacgata caaagattcg tgagaagtat tttgatatca
     9001 tgatggacag gattgaaaaa tttacttcag agaaaataag acaatttata gattataaaa
     9061 aaagttattg tatttccgat tttatatctg actataatgc atataaaggt aatgcttacg
     9121 gacttgcaaa taccttaaga caaacagcag ttttaaagcc aaaaatcaga agtaaaaaaa
     9181 taaataatct ttttttcaca ggacaattaa cagtgccggg tccgggcgta ccacctgcaa
     9241 ttttatcagg taaaattgca gcaaatgaat taattaaatc ttttagataa aatgaaaaca
     9301 ttatttgata atatttcggt cgaatgcagc aaacttgtaa ctaaaactta cagtacaagt
     9361 ttttcagcag gaatcagatt tcttcataaa gatctaagaa atcctgttta caatatctat
     9421 ggctttgtaa gatttgccga tgagattgtt gactcgtttc atgattttga taagaaggaa
     9481 ttacttgata aattttcaga tgatactttt gaagctattg aaaaaaaaat cagcctaaat
     9541 cctattctaa attcatttca ggatacagtc agaaaatatg aaattccaaa tgagcttatt
     9601 gagcagtttt tgagcagcat gagaatggat ctcaatagag tttcatacga cagtgatgaa
     9661 ttaaacgatt atattttagg atctgctgaa gtagttggct taatgtgtct gaaaatcttt
     9721 acatatgagg acaaaagttt gtatgaaaag ctaaaacctt ttgcaatgaa actcggatca
     9781 gcatttcaga aaattaattt ccttagagat cttaaaactg attataatga attgagcaga
     9841 agttattttc caaattttaa ccctgataat ttttcagacg acgataaaag gaaaatagaa
     9901 tctgatatag aaaatgaatt aataactgct cttgaaggaa taggtaaact acctgtctcc
     9961 tccagaaaag gtgtatatct tgcttatctc tattatttaa aactttttaa gaaaataaaa
    10021 agacttcctg ccaaagaaat aatgaagcga aggatcagaa tttccaatgg ggtaaaggca
    10081 ggtttggtta taaatttgtt tgctagaaaa ttagcaggtg aagtttaatt ttttataaaa
    10141 aatatatttt aaatataaat gtttgag
//
LOCUS       DAORVQ010000114        10095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3518_length_10095_cov_5.112151, whole genome shotgun sequence.
ACCESSION   DAORVQ010000114 DAORVQ010000000
VERSION     DAORVQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_10095_cov_5.112151"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLX80_09700"
     CDS             <1..188
                     /locus_tag="PLX80_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY21103.1"
                     /translation="LTGDVPSPSNVPSGCYFHPRCPKVFGDCSTIHPVLAGSESHMQS
                     CLLYPESYPKTNEERAA"
     gene            440..2170
                     /locus_tag="PLX80_09705"
     CDS             440..2170
                     /locus_tag="PLX80_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY21104.1"
                     /translation="MKKNYLIILAALMIFVSGCDKKEVAVMSDLPGGIKGGGIYKINE
                     LENIRSLDPAGISDVVSHHVAHQIYDLLIDLDTNLHVQPDLATRWEISEDGLLYTFHL
                     RDGVKFQNNQCFPEGKGREINATDVKYSFERICDPRAGTLGYDYYKNYVEGAKEYYEE
                     IALARKESRDIMIKEVTGYIAKDDSTFQIKLKSAFGPFIYYVTLAYCYIVPKEAVEHY
                     GKDYFQNPVGTGPFIFVDWKPDLELNLKRNPDYWAKDKNGNQLPYLEGVKFKFIKELS
                     QQMLEFQNGNLDECYRIPNESFKEVVTPEKTLTANFAKFRLQTVPSMGSQYYGFLTTG
                     KVFNNEKVRQAFNYAIDRDKILKFVLNGQGYMGAFHGLVPPSMPDYDIERIKGYDFNL
                     EKAKQLMTDAGYPGGKGFPEAVLQINSGGDRNIQIAESIQNMLKEIGVNMKLNIMQFA
                     QHLDNVDAGRADFYRAGWVGDYPDPENFLNLFYGKNVPKDSKQISPINSTRFQDAKFD
                     ELYEMGISKSDMKERYEYYYQAEQIAVYKAPMIFIYYDEHYRLLQPYVNGYGLDPMHR
                     VNFRTLWLNR"
     gene            2379..4103
                     /locus_tag="PLX80_09710"
     CDS             2379..4103
                     /locus_tag="PLX80_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY21105.1"
                     /translation="MKYIISVLIVILFSGCNNTKEKDNIVQLKGGIKGGGIYRINELE
                     NVRSLDPVRVNDDVSHHVTHQIYDLLVDLDKDLELVPELAQSWEVTEDGMLYTFHLRK
                     GVKFQDNACFPEGKGREFNAADVKYSFDRVCDPRSGSLGFDYYKNYVEGAEEFNEEVS
                     AAVKESRDPKFTGVSGYIVKDDSTFQVKLTKPFGPFIYYMCLGFAYVVPKEAVEKYGQ
                     DFFQNPVGTGPFIFVDWKQDLELNLKKNPNYWNKDEHGNQLPYIDGVKFKFLKETSQQ
                     LLEFRNKNIDESYRVPNESFKSIVTPEKTLTPEFSEFVLQKVPSLSIQFYGFLTTSKI
                     FNNEKVRQAFNYAIDRDKILKFVLNGQGYMGAIYGIVPPSMPNYDVEKINGYGFDLKK
                     AKQLMADAGYPDGKGFPETVLQINSGGDRNIQIAESIQSMLKELGVNMKLNILQFAQH
                     LDNIDAGRADFYRLAWIADYPDPENFLNLYYGKNVPKNPMDISPINSTRYQNPEFDEI
                     FEKAIATTDKDKRYELYLKAEQIAVSQAPMMLIFYEEDYILLQPYVKGYYLDPMHRVN
                     FRHLWLDK"
     gene            4216..4821
                     /locus_tag="PLX80_09715"
     CDS             4216..4821
                     /locus_tag="PLX80_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQY21106.1"
                     /translation="MPVLLVIFSVTILMGCSSTKELANDQDSRKNANGNNSKEVVSTS
                     SLTEKTSINKENLEAISSSLAGFLASPEYSGNLDKDEVMYKVIEYLNTPYLWGGTSKR
                     GIDCSAFIQTVVFQALGISIPRTSFEQSNVGEPIDRADLKFGDLVFFDTMRKGRVTHV
                     GMYLDNGYFTHSGSKTGVAVASLDDDFYSRTYLKARRVTEE"
     gene            4923..5849
                     /locus_tag="PLX80_09720"
     CDS             4923..5849
                     /locus_tag="PLX80_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21107.1"
                     /translation="MSAFIIRNFIYSIFIIIGVLTVTFCLLYIIPGDPARMMLGQRAD
                     VASVESVREELGLNKPIYVQYIKFMSNAFQGDLGRSYATNREVVQTILDKLPATALLS
                     LSALFISSILGILIGIISAVKQYTWLDNVSMIFALSGISFPSFAFGLIMALVFGHLLK
                     WFPISGYINEGPQYLVLPMLTLALRPMAITARITRSSMMDVMSQDYVRTAKAKGLSRS
                     KVIFKHTLRNALNPVVTTISASLAATLGGVFFIEYIFNWPGIGLLAIDAIFKLDFPMI
                     QGTVLFSAIVFVVINFFVDIVYALLDPKVKLS"
     gene            5900..6982
                     /locus_tag="PLX80_09725"
     CDS             5900..6982
                     /locus_tag="PLX80_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21108.1"
                     /translation="MEVETAVVADSILQEEQSKSLWHYSWKRLKRNKLAMTGLVTLCI
                     LIFIALTAPLIAPFNPNMQILEYSAKEINFKGTVFVKKDADTDSDEASYIPVKRIIRE
                     SGSEIVYMDFIDNEVTVNKDDIEETSEGWTKTLTYYLGTDRFGRDVLSRLIYGSRVSL
                     TVGLVSESIAIFLGLILGAIAGFYRGKVDVAVMWLVNVIFAFPAILLIIALSVVLGKG
                     LWQAFIAIGLTGWVDIARIVRGQFFSLRETEFVEATKAIGFNSVRVITRHILPNCMGP
                     VIVTGTVGLASAIIFEASLSFLGLGVQPPTASWGQMIFDGYKYIVVGTNWGLAVFPSI
                     AIMLTVFAVNLLGDGLRDAFDPKLKR"
     gene            complement(7025..7432)
                     /locus_tag="PLX80_09730"
     CDS             complement(7025..7432)
                     /locus_tag="PLX80_09730"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HQY21109.1"
                     /translation="MNEFNFSELNWLIGQWEGIQGNGIYHEEWTEPDKDKLTGKAYFI
                     SKGEISNPEKLTIISREDGIFYIADVSHNTAPVEFKLIDISENVLVFCNPLHDFPQKI
                     TYIKKGENNLTAIIEAEKNGKEKKIEFHLKKIQ"
     gene            7569..8018
                     /locus_tag="PLX80_09735"
     CDS             7569..8018
                     /locus_tag="PLX80_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQY21110.1"
                     /translation="MKIEDEIKQKKFNSEYTKLIINLVYTGNKMNFKTNELLKVKNLT
                     IQQYNVLRILRGQHPNPSTVNMIIDRMLDKMSNASRIIDKLEDKKLVVRKINFEDKRC
                     ADVMISEKGLELLKKLDSEINNNEKSFIHLSLQEVKILNDLLDKLRG"
     gene            8051..8731
                     /locus_tag="PLX80_09740"
     CDS             8051..8731
                     /locus_tag="PLX80_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQY21111.1"
                     /translation="MKKLKSLSVILMLIAVFVAAGCSDSKETDMTKKNSEKNENSANL
                     KNKPTVTNEGEKKEISAAKSNVEWVGKKVTGEHNGTVDITNGEIFVDENEVTGGSFEI
                     DFTTIKVLDIEDAESNTKLTNHLKSEDFFASDKHPKGKFNITSVSKVSGDKYNVTGDL
                     SIKGISKEITFPAVIKISDGIVTATADFEIDRTLWDIQYRSGKFFENLGDKLISDNFQ
                     IKFNITTL"
     gene            8734..9444
                     /locus_tag="PLX80_09745"
     CDS             8734..9444
                     /locus_tag="PLX80_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQY21112.1"
                     /translation="MKKRIFKSEDRGSADHGWLKAKHSFSFANYYDPEKIRFGTLRVL
                     NDDIVQPKMGFGKHPHDNMEIVTIPLKGELAHEDSTGNKEVIKQNEIQIMSAGSGLTH
                     SEFNNSNTEEVNLLQIWVFPKVKNIEPRYDQKVFDPSERKNKIHTVVSPEKNDGILWI
                     NQNAFFSLSDTDKDVTLKYEVKSEGNGVYFFVIEGKVEIDGEILNKRDAVGIEDTDSF
                     EINFKENSKVLFIEVPMN"
     gene            9482..10039
                     /locus_tag="PLX80_09750"
     CDS             9482..10039
                     /locus_tag="PLX80_09750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HQY21113.1"
                     /translation="MNKLKVIGISGSLRENSFNTALLKNAKELSKEFMDIRIYDISKI
                     PFYNHDLEKNGIPDEVEKFKSAIDESDALLFVLPEYNASVTGVIKNAIDWASRKVSPL
                     FEKPCAIMGASGGLSGTAGAQAHFRHIAVSVNMICMNRPAIQIRKSSTIFDENLLIKD
                     ETTLEHLDKYMKAFESWILNFTNKN"
BASE COUNT         3600 a         1442 c         1927 g         3126 t
ORIGIN      
        1 ttttgactgg cgatgttcct tcgccgtcaa atgttccatc gggctgttat tttcatccta
       61 gatgtccaaa agtatttggt gactgctcta ctattcatcc ggtattagcc ggcagtgaaa
      121 gccatatgca gagctgtctg ctttatcctg agagttatcc taagacaaat gaagaaagag
      181 cagcttagta atatttataa tagagcatac ttttgaaaaa tgaattaatt tataaattga
      241 aatcagattt taataagacg gttatttatc aaaatggtaa atgccgtttt tctattatgt
      301 agtattaatt cttgcaattt tcagtcggat aaaaaaacat aataatccaa attcaatttt
      361 attacttata aagtataatt tttttttaac aggtttaaat cattttagaa atttaataaa
      421 tcaataaaca aaattttata tgaaaaaaaa ttacctgatc atattagcag cactcatgat
      481 ttttgtaagc ggctgcgata aaaaagaagt ggctgtcatg tcagatctgc caggcggaat
      541 aaagggaggc ggtatctata aaataaatga gctggaaaat attagaagtc tggatcccgc
      601 aggtattagt gatgtagttt cacatcatgt agctcatcag atttatgatc ttcttatcga
      661 tcttgataca aatcttcatg ttcagccaga tcttgcaacg agatgggaaa tctctgaaga
      721 cggacttctc tatacttttc atctaagaga tggagtgaag tttcaaaaca atcagtgttt
      781 cccagaaggt aagggcaggg aaataaatgc aaccgatgta aaatattcct ttgagagaat
      841 atgtgatccg agagcaggta cacttggcta tgactattac aaaaattatg ttgaaggtgc
      901 aaaagagtat tacgaagaaa ttgctttagc cagaaaagaa tcacgtgata taatgataaa
      961 agaggttacc ggttatattg caaaagatga ttctactttt cagataaaat taaaatctgc
     1021 tttcggtccg tttatttatt atgtgacact tgcatattgt tatatagtgc caaaggaagc
     1081 tgtagaacat tacggaaaag attatttcca aaatcctgtc gggacgggac catttatttt
     1141 tgtagactgg aaacctgatc ttgagctgaa tttaaaaaga aatccggatt actgggcaaa
     1201 ggataaaaat gggaatcagc ttccatattt agaaggagta aagtttaaat ttataaaaga
     1261 gctgtcacag caaatgcttg aatttcagaa tggtaatctg gacgaatgct acagaattcc
     1321 taatgaatct ttcaaagaag ttgtaactcc agaaaaaact cttacagcaa attttgcaaa
     1381 gttcagactt cagacagttc catcaatggg gtcacaatat tatggtttcc ttactacggg
     1441 aaaagttttc aataatgaaa aagttagaca ggcatttaat tatgctatag acagagataa
     1501 aattttaaaa ttcgttttaa atggacaagg ttatatggga gcctttcacg gtctcgtgcc
     1561 gccttccatg ccggattatg atatagaaag aattaaaggt tatgatttta atcttgaaaa
     1621 agcaaaacaa cttatgactg atgcaggtta tccgggtggt aaaggttttc ctgaagcagt
     1681 tttacagatc aattcaggcg gagacagaaa tatacagatc gctgaatcaa ttcagaatat
     1741 gctgaaagaa attggtgtaa acatgaagct taatataatg cagttcgctc agcatcttga
     1801 taatgtagat gcgggaagag ctgattttta cagagccggc tgggtagggg actatcctga
     1861 tcctgaaaat ttcctgaatc tattttatgg aaaaaatgtg ccaaaggatt caaagcagat
     1921 cagccctata aattctacaa gatttcagga tgcaaaattt gatgaacttt atgaaatggg
     1981 aatatctaaa tctgatatga aagaaagata tgaatattat tatcaggcag aacagatcgc
     2041 tgtatataaa gcacctatga tctttattta ttatgatgag cattacagac ttttacagcc
     2101 ttatgtaaac ggatacggtc ttgatccgat gcatagagtg aatttcagga cactatggtt
     2161 aaatcgataa aattatattt aaaattctaa ttgcaaattg caaattacga gatacgagtt
     2221 acgagttacg agatacgaga tacgagatac gagatacgag atacatggaa attcaaaatg
     2281 agcttccaat ataaaattag tcaggcttga aaatattgaa tagtttaagt tttcagaaag
     2341 tgatgacaat ttttaaaaag aaaaaaacta taaaaaattt gaaatatata atatcagtac
     2401 tcattgtaat tttattttca ggttgtaata atactaaaga gaaagataac attgttcagc
     2461 tcaaaggcgg aataaaaggc ggcggcattt acaggataaa tgaacttgaa aatgtaagaa
     2521 gtctggaccc ggtcagagtc aatgacgatg tttcacatca tgttacacat cagatatatg
     2581 atttacttgt agaccttgat aaggatcttg agcttgttcc agagcttgca caaagctggg
     2641 aagtcactga ggatggaatg ctttatacat ttcatttgag aaagggggta aagtttcagg
     2701 ataacgcatg ttttccggaa ggaaaaggac gtgagttcaa tgcagcagat gtaaaatatt
     2761 catttgacag agtttgtgat ccgagatcgg gttcgctcgg atttgattat tataaaaatt
     2821 atgtggaagg tgcagaagag tttaatgagg aagtttcagc agcggtaaag gaatccaggg
     2881 atccaaaatt taccggtgtt tcaggttata ttgttaaaga tgattcgaca tttcaagtga
     2941 aattgacaaa gcctttcgga ccttttattt attatatgtg tcttgggttt gcttatgtag
     3001 tcccaaaaga agcagtggaa aaatacggtc aggacttttt tcagaatccg gtcggtaccg
     3061 gaccattcat atttgttgac tggaagcaag accttgagct taatttgaaa aagaatccta
     3121 attactggaa taaggatgaa catggaaatc aacttccata tatagatgga gtaaagttta
     3181 aatttctgaa ggagacatct cagcagcttc ttgaattcag aaataagaat attgatgaaa
     3241 gctatcgtgt accaaatgaa tcttttaaat ctattgtcac acctgagaaa actctaacac
     3301 ctgagttctc agaatttgtt ttgcagaagg ttccgtctct atcaattcag ttttatggat
     3361 ttctaactac cagtaaaatc ttcaataatg aaaaagtcag acaggcattt aactatgcta
     3421 tagacagaga taagattcta aagtttgtat tgaatggtca gggttatatg ggagcgatat
     3481 atggaattgt tccgccttca atgccaaatt atgatgtaga aaaaattaat ggatacgggt
     3541 ttgatctgaa aaaagcaaaa cagcttatgg ccgatgcggg ttaccctgac ggaaaaggat
     3601 ttccagaaac tgttttacag atcaactcag gtggtgacag aaatattcag atagccgagt
     3661 caattcaaag catgctaaaa gaacttggtg tcaatatgaa gctgaatata cttcagtttg
     3721 cacaacatct ggataatata gatgcaggaa gagcagattt ttacaggtta gcttggattg
     3781 cagattatcc tgatcctgaa aattttttga atctatatta tggaaaaaat gtacctaaaa
     3841 atccaatgga tataagcccg ataaattcta caagatatca aaaccctgaa tttgatgaga
     3901 tctttgaaaa agccattgct acaactgaca aagataaaag atatgaatta tatttaaaag
     3961 cggaacagat agcagtttca caagctccaa tgatgctgat attttatgaa gaagattata
     4021 ttctccttca gccgtatgta aaaggctatt atcttgatcc tatgcatcgg gtcaacttta
     4081 gacatttatg gcttgataaa tagaatgcaa gtaactaaat tgttcagtat tttttcaagg
     4141 aagaactaca attaaaacaa attttattaa ataaataagg atcagtaaat gattaaatta
     4201 aagctgttta aattaatgcc cgttttatta gttatatttt cagtaacaat attgatgggt
     4261 tgctccagta ctaaagaatt agctaatgat caggatagta ggaaaaatgc taatggaaat
     4321 aactcaaaag aagttgtgtc caccagcagt ttaactgaaa aaacctcaat caataaagaa
     4381 aatctcgaag caatatccag cagtctagca gggtttttag caagtcccga atattcagga
     4441 aatcttgata aagatgaagt aatgtataaa gtcattgaat atcttaacac accatactta
     4501 tggggtggta cttcaaaaag aggaattgat tgttcagctt ttatacaaac tgtagttttt
     4561 caggcacttg gtatttctat tccaagaaca tcatttgagc agtcaaatgt tggagagccg
     4621 attgatagag cagatcttaa atttggagat ctggtgtttt ttgatacaat gagaaaaggc
     4681 agagttactc atgtcggtat gtatcttgac aacggatact ttactcattc aggttctaaa
     4741 acaggtgttg ctgtagcttc tcttgatgat gatttttatt ccagaacata tctgaaagca
     4801 agaagagtaa ccgaagaata aaaagtattg aatttataat tttaaaatcc cggaatccga
     4861 taatacagga tttcgggatt ttttatattt tagtgttatt acaaaaaaag tattaaaata
     4921 atatgtcggc atttataatt agaaatttca tatactccat ttttattatt ataggtgtac
     4981 ttacagtaac tttttgtctt ctgtatatta ttcctggtga tccggcaagg atgatgcttg
     5041 gacagagagc tgatgtagca tcagttgaat ctgtcagaga agaacttggg ctgaacaaac
     5101 caatctatgt acaatacata aagtttatgt caaacgcttt tcagggagat cttggaaggt
     5161 catatgcaac aaacagagaa gtagtgcaga caatacttga caagcttcct gcaactgctt
     5221 tgctttcatt aagtgcatta ttcatttcat ctattcttgg cattctcata ggcattattt
     5281 ccgctgtaaa gcaatatact tggctggata acgtctcaat gatatttgct ctttcaggaa
     5341 tatcatttcc ttcatttgca ttcggtctga ttatggcact tgtatttggt catttgctga
     5401 agtggtttcc aatttcagga tacattaacg aaggaccaca gtatctcgtg ttaccaatgc
     5461 ttacacttgc tctcagacca atggcaataa cagcccgaat cacaaggtct tcgatgatgg
     5521 atgtaatgag tcaggattat gtgaggacag ctaaagcaaa aggactcagc cgtagtaaag
     5581 tcatatttaa gcatacttta agaaatgcat taaatcctgt tgtaacaacg atcagtgcat
     5641 cacttgctgc aacgctgggc ggtgtattct ttattgaata tatttttaac tggccgggaa
     5701 tcggacttct agcaatagat gcaatattta aacttgattt tccaatgata cagggaacgg
     5761 tattattcag tgcaattgtg tttgtagtga ttaatttctt tgttgatata gtttatgctt
     5821 tgcttgatcc aaaagtaaaa ttatcatgat aaaaatttct cttatttcat attttatact
     5881 ttataattat taataaaaaa tggaagtaga aacagcagta gtagcagata gtatcttgca
     5941 ggaggagcaa agcaaatcgc tttggcatta ctcctggaaa aggcttaaga gaaacaaact
     6001 tgcaatgaca ggtcttgtta ctctttgcat tcttattttt attgcactta ctgcaccgct
     6061 cattgcacca tttaatccca atatgcaaat actggaatat tcagcaaaag aaattaattt
     6121 taaaggaaca gtctttgtaa aaaaagatgc agatacagat tccgatgaag cttcttatat
     6181 tcctgtaaaa agaatcataa gagaatcagg aagcgagatt gtatatatgg attttataga
     6241 taatgaagtc actgttaata aggatgatat tgaggaaaca agtgaggggt ggacaaagac
     6301 attaacttat tatcttggga ctgacagatt tggcagggat gttttaagca gattgatcta
     6361 tggaagcagg gtaagtctta cagttggtct cgtttcggaa agtatagcca tttttcttgg
     6421 actgatactc ggagctattg caggctttta cagagggaaa gtcgatgttg cagtcatgtg
     6481 gcttgtaaat gtgatatttg catttccggc tattcttctg atcatagcgt tgtcagttgt
     6541 tcttggaaag ggactctggc aggcattcat tgccattggt cttacaggtt gggttgatat
     6601 tgcaaggata gtgagaggtc agtttttttc actaagagag acagaatttg tggaagcgac
     6661 aaaagctatt ggatttaatt ccgtaagagt aataaccaga catatacttc caaactgcat
     6721 gggaccagta attgtaacag gtacggtcgg tctggcatca gctataattt tcgaagcaag
     6781 tctgagcttc ctcggactcg gggttcagcc acctacagca agctggggac aaatgatatt
     6841 cgacggttac aaatatatag tggtcggtac aaactggggt ctcgcagtat ttccttcgat
     6901 tgccattatg cttacagttt ttgctgtaaa ccttttggga gacggattga gagatgcttt
     6961 tgatccaaaa ctaaaacgct gattttttaa atactgactt ccttagaaat atcgataata
     7021 aattctattg aatttttttc agatgaaatt caatcttttt ttccttacca tttttttcag
     7081 cttcaataat agcggttaaa ttattttcac cttttttaat ataagtgatc ttttgaggaa
     7141 aatcatgcaa aggattacaa aagactaaaa cattttcaga aatgtctatt aatttaaatt
     7201 ctaccggagc agtattatga ctgacatcag ctatataaaa aatgccgtct tcccgagata
     7261 ttattgtaag cttttccgga tttgagattt cacctttgct aataaaatat gctttacctg
     7321 tcaatttatc tttatccggc tcagtccatt cctcatgata tataccatta ccctgtattc
     7381 cttcccattg cccaatcagc caatttaatt cactgaaatt aaattcattc atataaaaat
     7441 tactttagtt aaaaaaaatt tataacaaag ataatcattc tgaaagaaaa tttaaacaga
     7501 agataatcta tgtatgtaca tataatttag tattgacata atatatgtat atacatatat
     7561 ttgtatgaat gaaaatagaa gatgaaataa aacagaagaa gtttaactct gagtatacaa
     7621 agctgataat taatcttgtt tataccggaa acaaaatgaa cttcaaaaca aatgagttac
     7681 taaaagttaa gaaccttact atacagcagt ataatgtatt gaggattctc agaggtcagc
     7741 atccgaatcc atctactgta aatatgataa tagacaggat gcttgacaag atgtcaaatg
     7801 catcaagaat tattgacaag ctggaggata aaaaactggt agtaagaaaa ataaactttg
     7861 aagacaaaag atgtgctgat gtaatgatca gtgaaaaagg attagaacta ttgaaaaaac
     7921 tagacagtga aataaataat aatgaaaaaa gttttatcca tttaagttta caagaagtaa
     7981 aaattttaaa cgatctttta gataagctga gaggttagta aaataaaaaa taaaaaataa
     8041 aaaataaaaa atgaaaaaat taaaatcatt aagtgtaatc ctgatgttaa tagcagtatt
     8101 tgttgcagca ggatgttcag attcaaaaga aacagatatg acaaaaaaga attctgagaa
     8161 aaatgaaaat tcagcaaatc tgaaaaacaa accgactgtt acaaatgaag gagaaaagaa
     8221 ggaaatttct gcagcaaaga gtaatgtaga gtgggtaggg aaaaaagtta caggtgagca
     8281 caacggtact gttgatatta ctaatggcga aatatttgtt gatgagaatg aagttacagg
     8341 gggaagtttt gaaattgatt ttactacgat aaaagtactc gatatagaag atgctgagtc
     8401 aaatacaaaa ctaacgaatc atttaaagtc agaagatttt tttgcatcag ataagcaccc
     8461 taaaggtaaa tttaatatta catcagtaag taaagtcagt ggtgataagt ataatgtcac
     8521 aggagacctt agtatcaaag ggataagtaa agaaattaca tttcctgcgg taatcaaaat
     8581 atctgatggt atagtgacag cgactgctga ttttgaaatt gacagaacat tatgggatat
     8641 acagtacaga tcagggaaat tttttgaaaa cctgggggat aaattaataa gtgataattt
     8701 tcaaattaaa tttaacataa ctacattata aaaatgaaaa aaagaatatt caaatctgaa
     8761 gacagaggaa gtgctgatca tggctggctt aaggcaaaac attcttttag ttttgcaaac
     8821 tattatgatc cggagaaaat acgtttcgga acactaagag ttctgaatga tgatatagtt
     8881 caacctaaaa tgggttttgg taaacatcca catgataaca tggagatcgt aaccattcct
     8941 ttgaaaggag aactcgctca tgaagacagc acaggaaata aagaagtaat aaagcaaaat
     9001 gaaattcaaa ttatgtctgc aggcagcgga ttgactcatt cggagtttaa taattccaac
     9061 actgaggaag tgaatttact tcagatatgg gttttcccaa aagtaaaaaa tattgagcca
     9121 agatatgatc agaaagtatt tgatccttct gaaagaaaaa ataagataca cacagtcgtg
     9181 tcacctgaaa aaaatgatgg tattctttgg atcaatcaga atgcattttt ctcactttcc
     9241 gatactgata aagatgtaac tttaaaatat gaagttaagt ctgaaggtaa tggtgtttat
     9301 ttttttgtaa ttgagggtaa agttgaaatt gacggagaaa tattaaacaa aagagatgca
     9361 gtagggatag aagacacaga ttcatttgag attaatttta aagaaaacag taaggtatta
     9421 tttattgaag ttccaatgaa ttaatgttat gtagtcaaaa taattatcat gatataaaaa
     9481 attgaataaa ttaaaagtaa ttggaatatc aggtagctta agggaaaatt cttttaatac
     9541 agctcttttg aaaaatgcta aagagctgtc aaaagaattt atggacatta gaatttatga
     9601 cataagtaaa attccgtttt ataatcatga tcttgaaaag aatggaattc cggacgaagt
     9661 agaaaaattt aaatctgcaa tcgatgaatc tgatgcattg ttatttgttt tgcctgaata
     9721 taacgcttct gttactggtg taataaagaa tgcaattgac tgggcttcca gaaaggtatc
     9781 acctttattt gaaaaaccct gtgcaattat gggagcttca ggcggacttt caggaactgc
     9841 cggagcgcag gctcatttca gacacattgc agtatccgtt aatatgatat gtatgaacag
     9901 acctgctatt cagatcagaa aaagcagcac tatatttgat gaaaatcttt taataaaaga
     9961 tgaaactact ttagaacatt tagataaata tatgaaagct tttgaaagct ggattttgaa
    10021 ttttacaaac aaaaattaat taaaattatt atgaaaatat tatatgtatt tccgcatcct
    10081 gatgacgaat ctttc
//
LOCUS       DAORVQ010000115        10075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3523_length_10075_cov_4.833034, whole genome shotgun sequence.
ACCESSION   DAORVQ010000115 DAORVQ010000000
VERSION     DAORVQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_10075_cov_4.833034"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /gene="leuS"
                     /locus_tag="PLX80_09755"
     CDS             <1..1866
                     /gene="leuS"
                     /locus_tag="PLX80_09755"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQY21114.1"
                     /translation="EELDWPKSVKEIQKNWIGKSEGSTVSFKINNPETGLFSDIEVFT
                     TRPDTIFGVTYLILSPEHKLVNDFTVSSKKADIENYRKEAERKSDMERTELSKNKTGE
                     FTGSYAIHPVTDEKLPVLISDYVLVSYGTGAIMGVPGHDERDNEFARKYSLKIIPVLH
                     PEEISNEEKENFIKDTVSGDVCFSDEGISINSDFLNGLKTTDAINKINEWLTANNKGK
                     KSVNFKLRDWLFSRQRFWGEPFPIVHFDENTFKDIDESELPLMLPEVDSYKQSGTGES
                     PLATITDWVNIVDTVSGMKVKRETNTMPQWAGSCWYFLRYLDPENTNAFCDKEIEKFW
                     MPINLYIGGMEHSVLHLIYARFWYKVLFDLGYVNYKEPVRKLFNQGMILGEDGVKMSK
                     SRGNVINPDDVIRDFGADAMRLFEMFMGPLEATKPWSTKGLEGLFRFLNRVWRLIIDE
                     STGNVKSNITDSEPTENLNKLLHKTIKKVSEDIEDGDMKFNTAISYMMIFVNELYKTE
                     EISKKLISDFILLLSPFAPHICEEIWERLGNKGSIQFENWPVFDEKFTKENTAIIIFQ
                     VNGKLRAKAELPFDSEEKVLEDVALENENVIKYLLGKQVVKIITVKNKMVNIVVK"
     gene            2078..2983
                     /locus_tag="PLX80_09760"
     CDS             2078..2983
                     /locus_tag="PLX80_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY21115.1"
                     /translation="MQTQELTNKLQNIGFKDNESHVFLVLLKGSAMSASEIAEKASIR
                     RTAVYEILKSFASRGFCNEIETNTILKYEMIDPEIIGDKIESELKKQSESRIENLKET
                     FGDFKNLYRSEESEENKKVNIELIRGFNKHRQEKFIELLNSAKNEILFMIRLEGYISE
                     ELDTSASEFYKKGGVIKSIYETSLNFKIIKDNARKNATTEDLLRICRKFEKSGEQVRI
                     SEKQLPNMTIFDRKIVFFNIHDKSIPRHSNSDIIIRNEDFASRMIDLFKFYWDISSDV
                     DSFKIVSKEEKSTKKKVSSKNSKVI"
     gene            3024..3779
                     /locus_tag="PLX80_09765"
     CDS             3024..3779
                     /locus_tag="PLX80_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21116.1"
                     /translation="MKTLNLKSVSAAFALITGFFLFTVSGILYDAQASPDYLTGKVQY
                     SDDHSPVTEGNVKIMTFDKSTRTYNVLETITIDQQGSFKVNRNLILTTDEIRIMAYPN
                     DVDNNGNPFEPKEINLQNYEYTENKNFEILIEVDRATSINSEIKMKIVKPEKSSEVRT
                     LTKESVSLDQNYPNPFNPTTVIRFDLPESKNVSLTVYNMKGESVAVLAENELMQKGLN
                     QYEFNAGNLPSGVYVYTLKAGNFTENKKMTLLK"
     gene            4032..4418
                     /locus_tag="PLX80_09770"
     CDS             4032..4418
                     /locus_tag="PLX80_09770"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HQY21117.1"
                     /translation="MYHNKHFTLSEASELLPKINLILKDIIESKRNLDNTNYNIYNHH
                     FFGGVGTNGSGKYPEDLVKLIDLVKKITDQGIQIKNLDNGLIDFPHIRENGEEVYLCF
                     VYGEDGLNFWHSIKDGFSGRRDISEL"
     gene            4452..5354
                     /locus_tag="PLX80_09775"
     CDS             4452..5354
                     /locus_tag="PLX80_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HQY21118.1"
                     /translation="MGIFNSVLIPVIETMPKSVVKIFANKYIAGDNINDAVTTVRNLN
                     KKGLMATVDVLGESITDKKEALISKDENIEALEAISKYDLDCNLSIKLTMLGLNIDQE
                     FCMILVSEILEKAKSVNRFVRIDMEDSSVTESTINVFENSRKKYNDVGIVIQAYLRRS
                     ENDVKRLSQMGANFRICKGIYIEPEKIAFKDPDEIRNNFMKILRIALENKSYCGIATH
                     DEILIKDSVKLVKELQLKNDEYEFQMLLGVRENLREEAVTKGHRMRIYVPFGKRWYEY
                     SIRRFKENPNIAGQVLKSIFSGGK"
     gene            complement(5382..6074)
                     /gene="thyX"
                     /locus_tag="PLX80_09780"
     CDS             complement(5382..6074)
                     /gene="thyX"
                     /locus_tag="PLX80_09780"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQY21119.1"
                     /translation="MKKEVLDNGFIEVLDKLGSDLTVVNSARVSFGKRKEVFDDNDRK
                     LVKFLAKNKHWSPFRHLMVQFHVKAPEFVMRQWYKHVVGIETSSSSSAKDHAWNEISG
                     RYVPVSDFYTPSNWRKQSDNNKQASEGSIEDQKYASEVFEEVMIYIKGSYEKLLDAGV
                     AKEQARMILPLNQYTEVYWTASFQAIMNFIELRDEATAQWEIREFAVAMKELMFEIYP
                     EITKIWFEVNSN"
     gene            6248..7186
                     /locus_tag="PLX80_09785"
     CDS             6248..7186
                     /locus_tag="PLX80_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQY21120.1"
                     /translation="MKLTESKFYIGADSGGTKFELLLVDLSGKILKSFRLKGIHYSVS
                     GPQIYSDKITEYIQTVVNKSGLEIKNCIGICIGLAGAREESDRTKLASLFKRKTQVRN
                     IIITSDAMTALIGAFEGKEGIILISGTGSVLYGFYDNKLKRIGGWGRIIGDEGSGYWI
                     GKKALNIATKEYDACENIKSQSKLCKILYSEFGIDRSNLNDKIFKENFDIQKITPVVL
                     DLASEKSKVCIEIIETAVTDLIYHIKTYIDFVKPERELKIALIGSLIENENILSQKLK
                     KEILKLKKVKTVEKKHSPAYGAYLLISGGSNLLIKI"
     gene            7188..8756
                     /gene="guaA"
                     /locus_tag="PLX80_09790"
     CDS             7188..8756
                     /gene="guaA"
                     /locus_tag="PLX80_09790"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQY21121.1"
                     /translation="MKKTDLILILDFGSQYTQLIARRTRELKVYSEIHTYKLNLKKFL
                     KDKEKNYNLKGVILSGGPASVTGKDSPKISKPDLDYFVKKKIPVLGICYGMQLLCSMY
                     KGSLHASLQREYGSTKVYIFKDSELFKGISSQLNVWMSHGDSIDKIPAKFIPTSKSIS
                     GVICSFENPMTKIYGLQFHPEVNHTESGRIILHNFLFGICLVKNLFEAGSFIENKIEE
                     IKKTSGKDNVICALSGGVDSSVAALLVSKAIGDKLTCIHIDNGLMRKNESRKVIELFE
                     KHYKLNLIFINASELFLKRLKGVFDPEKKRKIIGKTFIDVFEKEAKKLKGVKYLVQGT
                     LYPDVIESVTLKGPSSKIKTHHNVGGLPEKMDLKLIEPFRELFKDEVRDIGKTLGLRE
                     NFINRHPFPGPGLGIRILSDVTKEKLNILKEVDDIYINGLKEFGLYEKIWQAFSVLLP
                     VQTVGVMGDERSYENVIGLRAVTSTDGMTADFYAFDKEFLSHISNKIINSVKGVNRVV
                     YDISSKPPATIEWE"
     gene            8763..>10075
                     /locus_tag="PLX80_09795"
     CDS             8763..>10075
                     /locus_tag="PLX80_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY21122.1"
                     /translation="MNKKILIILLILFCIYGNGYTQIKIGVALPLMNNSDNKNDKNLG
                     SEILQGINDAVTEHNKNYPDNKVEIITEDTKRDPSTALEIINKFGSNNKVIAVFGPVF
                     SSELSPNAGAAAFHKIPLVTPTSTQSFLAEKNEFLFQLNPTYDIRGKTMAKFAFNELK
                     MNNFAIFSEDTYGKNFADSFESEVTLNGGEVTVKVFYSKDDEDLTQELEEMENKIFSM
                     DKFIDFGSLSVEQFEKIKNSETIFSYPDSLKDAGIIVSIYKLYGRNAVRITDSLKINS
                     TTNVDRKRTVMPGFVEAIYIPISTYLEIPKMISQYFSEHINLPVLGTSDWNNKETLEE
                     NKMYIKNLYIDSDFNLSDDADVPGGLSESGVKNYYFGYDGMKLLLDKIAEGNNNRNSL
                     NEALQNTENYKTTHNTVTLKDRTNHSLVIMRFRQGELMKVGDFTY"
BASE COUNT         3767 a         1367 c         1865 g         3076 t
ORIGIN      
        1 gaagagcttg actggccgaa gagtgttaaa gaaattcaga aaaactggat cggtaagagt
       61 gaaggatcta ctgtgagttt taaaattaat aatcccgaga caggtttgtt ttctgatatt
      121 gaagtattta caacaagacc tgatactata tttggtgtaa catatttaat tttatctcct
      181 gagcataaac ttgtgaatga ttttactgtc agctctaaaa aggcagatat cgagaattac
      241 agaaaagaag ctgagagaaa atctgatatg gaaagaacag aactttcaaa aaataagacg
      301 ggtgaattca caggctctta tgcaatacat ccggtcacag atgaaaaatt accggttctg
      361 atttcagatt acgtacttgt aagttatgga actggtgcaa taatgggagt acccgggcat
      421 gatgaaagag acaatgaatt tgcccgaaag tattctctta agattattcc tgtactacat
      481 cctgaagaaa tttcaaatga agaaaaggaa aattttataa aggatactgt aagtggggat
      541 gtttgttttt cagatgaagg aatttcaata aactcggatt ttctaaatgg attaaaaact
      601 acagatgcta taaataaaat taatgaatgg cttaccgcaa ataataaggg taaaaagtca
      661 gtaaatttca aacttcgtga ctggcttttt tccaggcaga gattctgggg agagccgttc
      721 ccgatcgtac attttgatga aaatacattt aaagatattg atgaatctga actgccatta
      781 atgcttcccg aagtcgattc atataagcaa agcggtacag gagaatctcc tttggcaaca
      841 attacagact gggtgaatat agtagataca gtttcaggaa tgaaagtgaa acgcgagact
      901 aatactatgc cgcagtgggc aggttcatgc tggtattttc tcagatatct cgatccggaa
      961 aatacaaatg cattttgtga taaggaaatt gaaaagttct ggatgccaat taatctttat
     1021 atcggaggta tggaacattc tgtgcttcat cttatttatg caagattttg gtataaagtt
     1081 ctatttgatc ttgggtatgt aaattataag gaacctgtga gaaaattatt caatcaggga
     1141 atgatacttg gagaagacgg agtaaagatg agcaagtcaa gaggtaacgt tattaatccc
     1201 gatgatgtga tcagagattt tggagcggat gcaatgagac tttttgaaat gttcatggga
     1261 cctttggaag ctacaaaacc atggagtacc aaaggcttag aaggactttt cagatttcta
     1321 aacagagtgt ggagacttat cattgatgaa tctacaggaa atgtaaaaag taatattaca
     1381 gattcagaac caacagaaaa tttaaataaa cttcttcata aaacaataaa gaaagtttca
     1441 gaagatattg aagacggaga tatgaaattc aatactgcta tttcatatat gatgatattt
     1501 gtaaacgaat tgtataagac agaagagatc tccaaaaaat taatttctga tttcattctt
     1561 ttgctttcac catttgctcc tcatatttgc gaagagatat gggaaagact tggaaacaaa
     1621 ggcagtattc agtttgaaaa ctggccggtg tttgatgaaa aatttacaaa agaaaatact
     1681 gctattatta tttttcaggt taacgggaaa cttagagcaa aggcagagtt gccgtttgat
     1741 tctgaggaaa aagttttgga agatgtggct cttgaaaatg aaaatgtgat aaaatatttg
     1801 cttggaaaac aagttgttaa aataattaca gtcaaaaata aaatggtcaa catagttgta
     1861 aaataaataa tcagatgaaa tttattaatg acaggtaaat ttaatttgcc tgtctttttt
     1921 tttaatttac atttgaaaaa actattgata tctctatatt ttaaagaata aattgtaaaa
     1981 aaaattatta atatttgaag aataaaggtc actaaaaaag ttcttgactt attaaattaa
     2041 atattgcaat attgtattaa atgaaaaaac aacaattttg cagacacaag agctaacaaa
     2101 taaattacag aatataggtt ttaaggataa tgaatctcat gtatttttgg tgttgttaaa
     2161 aggttcggcg atgagtgcat ctgaaattgc agagaaagcc tcaataagaa gaacagctgt
     2221 gtatgagata ttgaagtcat ttgcttcaag aggtttttgt aatgagatag agaccaatac
     2281 aattttgaaa tatgaaatga tcgatcctga aataataggt gataagatag aaagtgaact
     2341 taagaaacaa agtgaatcaa ggatcgaaaa cttaaaggaa actttcgggg attttaaaaa
     2401 tttatacaga tccgaagaat ctgaagaaaa caaaaaagta aatattgagt taattagagg
     2461 attcaacaaa cacagacagg aaaaatttat tgagttgctt aattccgcaa aaaatgaaat
     2521 tttattcatg atcagacttg agggatatat ttcagaggaa cttgatacat cagcaagtga
     2581 attttataaa aaaggcggag ttattaaatc aatatatgaa accagtctga attttaagat
     2641 cattaaagat aacgcaagga agaatgcaac gacagaagat ctgctgagaa tatgcagaaa
     2701 atttgaaaaa tccggtgagc aggttagaat ttcagaaaag caattaccga atatgacaat
     2761 ttttgacagg aaaatagttt tctttaatat acatgataag agtattccaa gacatagtaa
     2821 ctctgatata attataagga atgaagattt tgcttccagg atgatagacc tttttaaatt
     2881 ctactgggac atttcgagtg atgtggattc tttcaaaata gtttcaaaag aggaaaaaag
     2941 tactaagaaa aaagtaagtt caaaaaattc taaagttata taaaataaat aatttcatta
     3001 agtatttgac acaggagaaa aaaatgaaaa cattaaattt aaaatcagta tcagcagctt
     3061 ttgctttgat aacagggttc tttctgttta cagtatcggg aattttatat gatgcacaag
     3121 catctcctga ttatctgaca ggtaaagttc agtattcaga tgatcactct ccggtcactg
     3181 aaggtaatgt caaaattatg acttttgata aatctacaag aacttacaat gtacttgaaa
     3241 cgattacaat tgatcagcaa ggttctttta aagttaacag gaatctgatc ctaactactg
     3301 atgagattag gataatggcg tatccaaatg atgttgacaa taatggaaat ccctttgaac
     3361 ccaaagaaat taatcttcag aattatgaat atacagaaaa caaaaacttt gaaattctca
     3421 tcgaggttga cagggcaact tccattaatt ctgaaataaa aatgaaaatt gtcaaacctg
     3481 aaaaatcttc tgaagtcagg actttgacaa aagaatctgt aagcctggat cagaattatc
     3541 caaatccatt taatccaact acagtaataa gatttgacct tccggaaagt aaaaatgtat
     3601 cactaacagt gtataacatg aaaggtgaat cagtcgcagt acttgcagag aatgagctga
     3661 tgcaaaaagg tttaaatcaa tatgagttta atgcaggtaa tttaccaagc ggtgtttatg
     3721 tttacacact taaagcagga aattttacag agaataaaaa aatgacactt ttaaaataat
     3781 aacgtccttg aaagcaagaa agatttaatg agggttgggt ctttgcttgt gaaatcccct
     3841 ctcagggaaa ggaggggatt cagggatgtt tataattccc gaaatacaat cgatttaaaa
     3901 ctattatcta aaagattttc tcttccactc acaaaaaagc attttgatta atttcaaaat
     3961 gctttttttt taccttattt atcaaatcat ttagaaatat tttgcaaaat aaattttgaa
     4021 ctttcttatt aatgtatcat aataaacatt tcactctctc agaggcaagc gaacttctac
     4081 ctaaaatcaa tctgattctt aaagatataa tagagtctaa aagaaattta gataatacaa
     4141 attataatat ttacaaccat catttttttg gaggtgttgg aactaatgga tcagggaaat
     4201 atcctgagga tcttgttaaa cttatcgatc tggtaaaaaa aattacggat caggggattc
     4261 agataaaaaa tcttgacaat gggcttatag attttccgca tatcagagaa aatggtgaag
     4321 aagtttatct ctgttttgtt tatggagagg atggactgaa tttctggcat tcaataaaag
     4381 atggattcag cggcagaaga gatattagcg aactttaatt atcagttaac ttattgaaaa
     4441 attattaatc tatggggatt tttaacagtg tattaattcc ggtcattgaa acaatgccga
     4501 aaagtgttgt caaaattttt gcgaataaat atatagcagg tgataatata aatgatgcag
     4561 taactacagt tcgaaattta aataagaaag gactaatggc tactgtagat gtccttggag
     4621 aatctatcac agataaaaag gaagcattaa tctcaaaaga tgaaaacatt gaagcattag
     4681 aagctataag taaatatgat ctggattgta atctttctat aaaactgaca atgcttggtc
     4741 ttaatataga ccaggaattt tgtatgattc ttgtgtctga aattcttgaa aaagccaaaa
     4801 gtgtaaacag atttgtaaga atagatatgg aggattcttc agttactgaa tctactataa
     4861 atgtctttga gaattcgaga aaaaaatata atgatgttgg aattgtgatt caggcatatc
     4921 taaggagaag tgaaaatgat gtgaaacgac tttcacaaat gggtgcaaat ttcaggatct
     4981 gtaaaggaat ttatatcgaa cctgaaaaga tagcgtttaa agatcctgac gagataagaa
     5041 ataattttat gaaaattctt cgcatagctc ttgaaaataa atcctattgc ggaattgcaa
     5101 cacatgatga aattttgatc aaagattctg taaaacttgt aaaagagctt cagcttaaaa
     5161 atgatgaata tgaatttcaa atgctgctcg gtgtaaggga aaatctcagg gaagaagcag
     5221 ttaccaaagg tcacagaatg agaatttatg ttccctttgg aaaaagatgg tatgaatatt
     5281 ctataagaag gtttaaagaa aacccgaaca tagccgggca ggttttaaaa agtatttttt
     5341 ccggtggaaa atagtttttc agtgaaagca aaatttcata tttaattgga atttacttca
     5401 aaccagatct tagtgatctc aggataaatt tcaaacataa gctctttcat tgcgactgca
     5461 aattctctta tttcccattg agcagttgct tcatctctta attctataaa attcataatc
     5521 gcctgaaaag aggcagtcca gtaaacttca gtgtactgat taagcggaag tatcatccga
     5581 gcctgttctt ttgccacacc ggcatctaat agtttttcat aagatccttt tatataaatc
     5641 atcacttctt caaaaacttc actggcatat ttctgatctt caatgcttcc ttccgatgcc
     5701 tgtttattat tatctgactg ctttctccag tttgaaggtg tatagaaatc actaactggt
     5761 acatatctgc cgctgatctc attccatgca tgatcctttg ctgaagagct tgaggaagtt
     5821 tcgatgccta caacatgttt gtaccattgt ctcattacaa actcgggcgc tttgacatga
     5881 aactgtacca ttaaatgtct gaatggagac cagtgtttat ttttagccaa aaatttaaca
     5941 agttttctgt cattatcatc aaacacttct tttctttttc caaatgaaac tcttgcggaa
     6001 tttacaacag taagatctga gcccagctta tcaagtactt ctataaaacc attgtctaat
     6061 acttcttttt tcaaattttt agggagttaa ttttttttga aaataaatat aatgaattta
     6121 atcgggagtt tggattttaa aatatttgaa tattatatga aatatttttt aaacgtaaat
     6181 taatttttta tcaataataa attctttgta attttaattt gattctgtaa ttaataactt
     6241 gctgaaaatg aaacttactg aatcaaaatt ttatatcgga gcagattccg gaggcacaaa
     6301 gtttgaattg ttgctggtgg atctgtcggg gaaaatcctg aaaagtttca gattaaaagg
     6361 aattcattat tcagtatccg gtccccaaat ttattcggat aaaataacag aatacattca
     6421 aacggttgtc aataaatcag gacttgaaat aaaaaactgc attggaattt gtataggtct
     6481 agcaggagca agagaagaat ctgacagaac aaaattagca agtttgttta aacgaaaaac
     6541 tcaggtaaga aatattataa taacttctga tgcaatgaca gcactgatcg gagcttttga
     6601 aggaaaagaa ggtataattc tgatcagcgg gacagggtct gtattgtatg gcttttatga
     6661 taataaatta aaaagaattg gcggatgggg caggatcatt ggagatgaag gcagcggata
     6721 ctggataggt aagaaagctt taaatattgc gactaaagaa tacgatgcat gtgaaaatat
     6781 taaatctcaa agtaagttat gtaaaatatt atattccgaa tttggaattg acagatcaaa
     6841 tcttaatgat aagatattta aagaaaattt tgatattcaa aaaattacac ctgttgtact
     6901 ggatctagct tctgaaaaat caaaagtctg cattgagatc atagaaactg ctgtaacgga
     6961 tttgatatat catataaaaa cttatataga ttttgtgaaa cccgaacgcg aattgaaaat
     7021 tgctttgata ggaagtctga tagaaaatga aaacattcta tctcaaaagc tgaaaaaaga
     7081 aattttgaaa cttaagaaag ttaagacagt tgaaaaaaaa cattctcctg cttacggagc
     7141 atatttgctg atctcaggcg gatcaaattt attaataaaa atttaatttg aaaaaaacag
     7201 atcttattct gattttagat ttcggatcac aatacactca gcttatcgca aggagaacga
     7261 gggaattaaa agtttattca gagattcaca catacaaatt aaatttaaaa aaatttttaa
     7321 aagataaaga aaaaaattat aatctaaagg gagttattct atccggagga cctgcaagtg
     7381 ttacgggtaa agactctcca aaaatttcaa aacccgatct ggattatttt gtaaaaaaga
     7441 aaatacctgt tcttggtatt tgttatggaa tgcagcttct atgcagcatg tacaaaggaa
     7501 gtttgcatgc atcactgcaa agagaatatg gaagcacaaa agtttatatt ttcaaagatt
     7561 cggaattatt taaaggaatt tcctcacagt taaatgtctg gatgagtcac ggtgattcta
     7621 ttgataaaat acctgcgaaa tttatcccaa cttcaaaaag tatcagcggg gtaatctgca
     7681 gttttgaaaa tccgatgacc aaaatatacg gattgcagtt tcatccggaa gttaatcata
     7741 cagaatctgg aaggatcata cttcataatt ttttattcgg aatctgctta gtaaaaaatt
     7801 tatttgaagc aggatctttt attgaaaata aaattgaaga aattaagaag acttcaggta
     7861 aagacaatgt aatctgtgca ctcagcggcg gagttgattc atcggtagct gcactattgg
     7921 tgagtaaagc gatcggtgac aaactgacct gtattcacat agacaacggc ctgatgagaa
     7981 aaaatgaaag caggaaagtt atagaacttt ttgaaaaaca ttacaagctt aatcttattt
     8041 ttataaacgc ttctgaactt tttttaaaaa gattaaaagg tgtttttgat cctgaaaaaa
     8101 aacggaaaat aatcggtaaa acttttattg atgtttttga aaaagaagca aaaaaattaa
     8161 aaggagtgaa gtatcttgta cagggcactc tttatcctga tgtaatcgaa tcggtaactt
     8221 taaaaggtcc ttcatcgaaa ataaaaacac atcacaatgt aggtggattg ccagagaaaa
     8281 tggatctgaa acttattgaa ccttttagag aattatttaa agatgaggta agagacattg
     8341 gtaagacttt agggctaaga gaaaatttta taaacagaca tccgtttccc ggacccggtc
     8401 ttggtatcag aatactcagc gatgttacta aagaaaaatt aaatattcta aaagaggtgg
     8461 atgatattta cataaatggt ttgaaagaat ttggattata cgaaaagata tggcaggcat
     8521 tttcagtttt actgccggtc cagacagtag gtgtaatggg cgatgaaaga agttatgaaa
     8581 atgtaatagg tctcagagct gtaacttcca cagacggaat gacagcagat ttttatgctt
     8641 tcgacaaaga atttttatca catatatcaa ataaaattat taattcagta aaaggtgtaa
     8701 accgagttgt atatgacatc agctctaaac cgcctgccac aatagaatgg gaataaaaaa
     8761 ttatgaataa aaaaatatta attatattac tgatactgtt ttgcatttat ggaaatggtt
     8821 atactcagat aaaaatcggt gtagctctgc cattaatgaa caattcagat aacaaaaatg
     8881 acaaaaatct gggttcagaa atattgcagg gaataaatga tgcagttaca gaacacaata
     8941 aaaattatcc agataataaa gttgaaataa tcaccgaaga tacaaaaaga gacccttcta
     9001 cagctcttga gatcataaat aaattcggaa gtaataataa agtaatagct gtatttggtc
     9061 cggtgttcag ttcggaactc tcaccgaatg caggtgctgc tgcgtttcat aagattcctc
     9121 ttgttactcc aacttcaaca cagagctttt tggctgagaa aaacgagttt ctgtttcaat
     9181 tgaatccgac ttatgatatc agaggaaaga caatggctaa gtttgctttt aatgaactta
     9241 agatgaataa ttttgccata ttttcagaag atacatatgg taaaaatttc gcagacagtt
     9301 ttgaaagtga agtcacattg aacggaggcg aggtcacagt aaaagttttt tattcaaagg
     9361 atgacgaaga tcttactcag gagcttgaag aaatggaaaa caaaatattt tcaatggata
     9421 agttcataga ttttggaagt ctcagtgttg agcagtttga aaagataaaa aattctgaaa
     9481 ctatattttc atatccggat tccttaaaag atgcaggtat cattgtcagc atttataagc
     9541 tttacggaag aaatgccgta agaattactg actcattaaa aattaacagt actacaaatg
     9601 tggacaggaa gagaacagta atgccgggtt ttgtagaagc aatttatatt ccgatatcta
     9661 cttatctaga gatcccgaaa atgatctctc agtatttttc cgagcatata aatttaccgg
     9721 tacttggtac cagtgactgg aataataaag aaacacttga agaaaataaa atgtatatca
     9781 agaatctgta tattgattca gattttaatc tgagcgatga tgcagatgtt ccgggaggat
     9841 tatcggaaag cggagtgaag aattattatt ttggatatga cggaatgaaa cttttacttg
     9901 ataaaattgc tgagggcaac aataacagaa attcactaaa tgaagctttg caaaacacag
     9961 aaaattataa aacgacacat aatactgtta cattaaaaga cagaaccaat catagtctgg
    10021 tgataatgag attcaggcag ggtgagctga tgaaggtcgg ggattttact tatta
//
LOCUS       DAORVQ010000116         9980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3582_length_9980_cov_8.131788, whole genome shotgun sequence.
ACCESSION   DAORVQ010000116 DAORVQ010000000
VERSION     DAORVQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3582_length_9980_cov_8.131788"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4453)
                     /locus_tag="PLX80_09800"
     CDS             complement(<1..4453)
                     /locus_tag="PLX80_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQY21123.1"
                     /translation="MKKSTYFFELLVIFIFCLSSGNLFSQEENILPREFKADEQRMKS
                     NSGTPSVPTIQSNSPTIIINVPANYSTIQAAINAANNGDIITVDAGIYREDITLNKYL
                     QIRGANYTINPNTGIRITETIIQPGTSDPDNNTFFYITPSGSGSTIDGFTFDGDNNLI
                     VSAVNINGANIDAAEAIGAYDGLSNTTISNNIIKNLNYGGIDLYNYYNGGAATYDNVV
                     TNNKFDNIIPNVFGTAVIIYNNCYTSVTNNVMTRVRVGVQTGNYNQPDLGSNHSISNN
                     TIESSRNAIFHNLVYSNASTFIIDNNIMTTLAGSTDNIGIRFSSIQSAVAVSMNNNSV
                     ADAWTGYMLWNCPTSSTITVTGSSASNCNIGVFANNYDGYSSNAATSIYAMTGMTLTN
                     CDTSIWIRDNQLNSNSATVLLNISNVTNLVNGNGIGLLIEGGDASVAFNGAVPVNFNS
                     TLNTYIRLATNGINLPTGTIDAQSVLFDGVTGPASTNTQLFAVEDKIDHKSDWNSLGF
                     VNVKAFNNYTTPNSFYPPNTTAALIQNGINIASAGDTVNVGTGTYNENVLLNKAVTLK
                     GNGPANTILTPAISCTGNGITISSPNSNVNDLTVTDFTYGIVTSASTINLYNVESNLN
                     CSYGISTGNGTNNLNIVKCKLNNNLIGGWRAGTADQLTDILLDSCEVKGNGVAVNSGF
                     GIFLAASSSVTNICDNITIKNSDFSNNLEKGMYFEKLRNALIDNVVMDNSGNDPGYGF
                     NNGIDINLKYGSYSNITIQNSSITNCGATGTATDVNNPAAITVKARDDAPSYNSNPAT
                     LTGFVMKNNFVTGPNNGLRFGEFNKVNNSPTGNTIEENNFGNGYSNKAIVNKTSNTIT
                     VSCNWYGTTMPTAVVALHGSSLNFLPYLVNGTDNDLGMPGFQIVPGSCTGAGPVNNLT
                     QATNYIAIQPAIDAANPGDTLEASAGTYNERVVITKPLTLQGADKITTILSGSTFPDS
                     GRGILVSNGITNVNIRNMTVQNFAGTNGNTHAGIYAIGGNNNLSVTNVIIQNNVGGSG
                     FYANGPINTVLIDSVTASGHTANARGIVIWNGLKENITITNCEVFNNNCCGIELQDGT
                     ASGVTISNNNVHDNFDNGIGIVGLQGPGENVVSNNTLLNNGRFGIEVKNPNGSGLATG
                     PGRVVIENNNVSRNTGLADVRDLAGIAVFRRGVLPGNVDVPNGAVVQNNTVSDYIQAG
                     VGEGFGIVVEGTSHTVIGNNVSGCDVGIQKQAGHLPYPGDGNQNDTNTYFGRGNSPVT
                     CGVTVTGNILSNTIDTRDVGANVNGGIVQNFNTSEIFCSIQAAVNDAQTLDGHTINVS
                     PGTYNEQVLVTKSLYIKGLGATQPVIDFTGTVSGKPTLFDISKAGVTIENMRFRVDQT
                     KLKSAIIASAGTMNNITVRKDSIEAYASSNVPSVLPYGDWNAISINYGGSTNYRIASG
                     GVNNILVDSNTVSGVANDGFGNPRFFRAAVAVDEGGGTFSNNVMQSIN"
     gene            5494..7587
                     /gene="recG"
                     /locus_tag="PLX80_09805"
     CDS             5494..7587
                     /gene="recG"
                     /locus_tag="PLX80_09805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQY21124.1"
                     /translation="MDIKFLKGVGEKRAEAFSKIGIETLDDFLNFIPRTYLKKINIRE
                     IPSNINENVIISGKVIDLEHPRKSNHPYRVVLRDNTGTAAIPIFGASEFRSRQFRLND
                     DFIFWVKIEKDNFASVPKISYRDHLKINTVDPIDLDFTKFSLIPVYELSGILKKTWIK
                     PLLLSKIVFNAFNTMLKSNPDYIEETLPEDIVSRYKLSGKKEAVLRINFPVEFSDIEF
                     SRKRLSFEELFYLELILALKKKSLENEKKGIKFNNDIHVITDKFKSVLKFELTNAQKK
                     VMNEIYLDMNSDKCMNRLLQGDVGSGKTIVAVYSILIAIENGYQAAFMCPTELLAEQH
                     YKTISELLKPLDINVCLLVGGQKKKLRAEILEGISSGKTNLIIGTHALIQETVEYKNI
                     GFVVIDEQHKFGVMQRAKLKEKGFNPDVLVMTATPIPRTLSMTYYGDLDVSVIDELPK
                     NRIPVKTALRSESDKPAIYKFVREEIKKGRQVYIIFPIIDESEKLDLKSAEENYLILK
                     NDIFPDMKVGMVHGRMLWYEKDEEIENFRDSKMNILVATTVIEVGIDIPNATVMIIEE
                     AQRYGLSQLHQLRGRVGRGADQSYCILIAKDLSEDSAKRLNILCKSTDGFKIAEADMD
                     IRGPGEFFGIRQSGILNFSCTDLKKDKDVLLIARDIAFKIIDEDPQLRKNVHSVIKKT
                     FLKNYKDSVYLTDVA"
     gene            7758..9179
                     /locus_tag="PLX80_09810"
     CDS             7758..9179
                     /locus_tag="PLX80_09810"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HQY21125.1"
                     /translation="MKSRSTKIEVIIPLLTVLSDAVSIFGAFLISYWIRFYSPLTKII
                     PVTKGIPDISGYIEFSLIVIPIWLIIFQTRKMYRLKRNVFIFDEFFVIIKCISIGVLI
                     AMGLVFILKGDFPYSRLVFFLIWLFSIFIVTVGRYFLLKIEKNLYNNDKGVNNVAILG
                     TNLMAKNIYENFSRDKFTGYHITGYFSYGENSTGEMEGKKYHGDYILIPDMLQELNLD
                     KIFISIPSSEHDILYNLFKICEGKNIEFMYAPDFIDIMTSRLRIEEVDGIPFMKLKSL
                     PMNVWNRFTKRTFDIVVSFLALLFLSPLMIVLSILVKLTSKGPLFYNQERVGLDEQKF
                     IMLKFRSMKIDAEKKGPQMTTRDDDRYTPIGKILRKYSLDELPQFYNVLIGNMSIVGP
                     RPEREFFINSMKHSIRRYLERHRVKGGMTGWAQVSGLRGTDSSMQTRIDYDIYYIENW
                     SIAFDIKIIFKTIKEVLFSKEAF"
     gene            9183..9551
                     /locus_tag="PLX80_09815"
     CDS             9183..9551
                     /locus_tag="PLX80_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21126.1"
                     /translation="MIIAVIYFIHVIFAVYAFSKSYQNDGLLQAFLNLGFIITLFAVS
                     LTVCELIVGLFISDTGYNMLLPVNSVLLFFLKISGFYAQQANVITLTPKDTITLILVT
                     ILEFSFYKFYFKKTEITKIA"
     gene            9573..>9980
                     /locus_tag="PLX80_09820"
     CDS             9573..>9980
                     /locus_tag="PLX80_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21127.1"
                     /translation="MSFKKLSLLFILISAFVAYSFSSRLHNTVQNTSSSRDIALQFIT
                     YNGYGNNLYIDNVLTGERISNDVAVTSIINFPIDSVYSIYTSGTDTVAPILSVANIGL
                     SNSSDSITVHMKIDPGNYLRDTLVRGLAPGQTAF"
BASE COUNT         3072 a         1778 c         1739 g         3391 t
ORIGIN      
        1 gattgatcga ctgcattaca ttgttgctga atgttccgcc gccttcatcc actgcaactg
       61 ctgctctgaa gaatctcgga ttgccgaaac cgtcatttgc cacaccggat actgtattgc
      121 tgtccaccag tatattgttc actccgcctg aggctattct gtaattcgtg cttccgccgt
      181 agtttatgct gatagcattc cagtctccgt atggaaggac agatggaaca ttgcttgaag
      241 catatgcctc tattgagtct tttcttactg taatgttatt catcgttccg gcgctagcta
      301 ttatagcact ctttagtttt gtctgatcca ctctgaatct catattctcg atcgtaactc
      361 cggcttttga tatgtcaaac agtgtcggct tgcctgatac tgttcccgtg aaatctatca
      421 ccggctgtgt ggctccgagt cctttgatgt aaaggctctt tgtcacaagc acctgctcgt
      481 tgtatgtacc cggacttaca ttgatcgtat gtccgtctaa cgtctgcgca tcattgactg
      541 ctgcctgtat cgaacagaat atctcgcttg tgttgaagtt ctggactatg ccgccattta
      601 catttgctcc gacgtcacgt gtatcgatcg tattcgaaag tatatttccg gttaccgtca
      661 cgccgcatgt cacaggtgag tttcctctgc cgaaatatgt attggtgtca ttctgatttc
      721 cgtcgcccgg atatggaaga tgtcccgcct gtttctgaat accgacgtca cagccggaaa
      781 cgttattgcc gatgactgtg tgacttgttc cttcgaccac tattccaaat ccttcgccga
      841 ctcctgcctg tatgtaatca gagactgtat tattctgtac cactgcgccg ttgggtacgt
      901 ctacattacc cggcaatacg cctcttctga atactgcaat acctgcaagg tctctgacat
      961 ctgcaagtcc tgtattcctt gacacattat tattttcaat gacaactctt cccggtcctg
     1021 ttgcaagtcc tgatccgttc ggattcttta cttcaattcc gaatctgccg ttgttgagaa
     1081 gtgtattgtt gcttacaaca ttctcacccg gtccctgaag tccgactata ccgatgccgt
     1141 tgtcaaagtt gtcatgtaca ttgttgtttg aaattgttac gcctgatgca gttccgtcct
     1201 gaagctctat tccgcagcag ttgttattaa atacttcgca gttggtgata gtaatatttt
     1261 ctttaagtcc gttccatatt actatacctc ttgcatttgc tgtatgacct gatgctgtca
     1321 cagaatctat caatactgta ttgatcggac cgttggcata aaatccgctg ccgccgacgt
     1381 tgttctgaat gatcacattg gttactgaca aattattatt cccgccgatc gcataaattc
     1441 ctgcatgcgt gtttccgttt gttcctgcaa aattctgtac cgtcatgttg cgtatgttca
     1501 cattcgttat accgttactt acaagtattc cgcggccaga gtcagggaag gtcgagccgg
     1561 aaagtattgt agtaatttta tcagctccct gtaatgtaag aggttttgta attaccactc
     1621 tttcattgta tgtaccggca cttgcttcca gtgtatctcc cggattagca gcatctatcg
     1681 ccggttgaat tgctatataa tttgtagcct gtgtcagatt attaaccgga cctgcgcctg
     1741 tacatgatcc cggtacgatc tgaaatccag gcattcccag atcattatct gttccgttta
     1801 ctaagtatgg caggaaattc aaactgcttc cgtgcaaagc tacgactgcc gtcggcattg
     1861 tagtgccgta ccagttgcag cttactgtaa ttgtatttga agtcttgtta acaattgctt
     1921 tgtttgaata accgttaccg aaattatttt cttcaatcgt atttccggta ggtgaattat
     1981 ttactttatt aaattctcca aatcttaaac cattatttgg tccggttaca aaattatttt
     2041 tcataacaaa tcctgtaagg gtagcaggat ttgaattata tgaaggagca tcatctcttg
     2101 ctttgactgt aattgcagca ggattattta catcagttgc tgtaccggta gctccgcaat
     2161 ttgtaatact cgaattctga attgtgatat ttgaatatga cccgtatttc agattaatat
     2221 caattccgtt attaaaccca tatccgggat cattaccgct gttgtccatt acaacattat
     2281 ctatcaatgc atttctgagt ttttcaaaat acattccctt ttcaagattg ttgctgaaat
     2341 cagaattttt aattgtaatg ttatcacaaa tatttgtgac tgatgatgaa gctgcaagga
     2401 atattccaaa tccgctgtta accgcaactc cgtttccttt tacttcacag ctgtctaaga
     2461 gaatatctgt aagctgatca gcagttcctg ctctccagcc tccaattaaa ttattattta
     2521 atttacattt tacaatgtta agattgtttg ttccgttgcc tgtgctaatt ccgtaactgc
     2581 agtttaaatt actttccaca ttataaagat ttatcgtgga agctgaagtc actattccat
     2641 aagtaaaatc tgtaactgta agatcattta catttgaatt tggagaggaa attgtaattc
     2701 catttcctgt acatgaaatc gctggagtta agattgtatt agccggtccg ttacctttca
     2761 aagtaactgc cttattcaat aaaacatttt cattgtaagt acctgtacca acatttacag
     2821 tatctcccgc tgatgcaata ttaattccgt tttgtataag tgctgcagtt gtattcggag
     2881 gatagaaact gtttggagtc gtataattat taaatgcttt cacattaaca aatccaagac
     2941 tgttccagtc actcttatga tcaattttat cttcgaccgc aaataattgt gtgttggtcg
     3001 aagccggtcc ggtgacgcca tcgaagagaa cgctttgagc atctatagtt cctgtcggta
     3061 aatttattcc gttggttgct aatctgatat atgtattaag tgtagagtta aaattaacag
     3121 gcactgcgcc attaaatgca actgatgcat cacctccttc tatcaataat ccaataccgt
     3181 ttccgtttac taaattggta acattactaa tatttagcaa tactgtagca ctgtttgaat
     3241 tcagttgatt atcacgaatc catattgaag tatcacaatt tgtaagagtc atacctgtca
     3301 ttgcatagat acttgttgct gcattggatg aatatccgtc atagttattt gcaaagactc
     3361 cgatattaca atttgatgct gagctgcccg tcacagttat tgtagatgaa gtcggacaat
     3421 tccataacat atatcccgtc catgcatctg ccacactgtt attattcata ctcacggcaa
     3481 cagcactttg aatagaagaa aaacgaattc caatattatc tgttgaccct gcaagagtgg
     3541 tcataatatt gttatctata ataaaggtag aagcatttga atacacaagg ttatgaaaaa
     3601 ttgcatttct tgaagactcg attgtattat tgctgatcga atggtttgac ccaaggtcag
     3661 gctgattata attccctgtc tgtactccga cccttacccg tgtcattaca ttatttgtaa
     3721 ctgaagtata acaattatta taaattatta ctgccgtacc aaatacattt ggtattatgt
     3781 tatcaaattt attgtttgtg actacattat cataagtagc cgcaccgccg ttataataat
     3841 tataaagatc aattccacca taattcagat tctttataat gttattactt atggttgtat
     3901 ttgacagtcc gtcatatgca cctatcgcct cggctgcatc aatgtttgcg ccgtttatat
     3961 ttacagcact cacaataaga ttattgtctc cgtcaaaagt aaatccgtct attgtagatc
     4021 cgcttcctga tggtgtaata taaaaaaatg tattgttatc aggatcagaa gtacccggct
     4081 gaattatagt ctcagttatt cttatccctg tatttggatt aattgtgtaa tttgccccgc
     4141 ggatctgtaa atacttgtta agagtaatat cctctctgta aatacccgca tccactgtaa
     4201 ttatatcacc attgtttgct gcattgattg cagcctgtat ggtagaataa tttgcaggga
     4261 cattgatgat gattgttggt gagttacttt gaattgttgg aacagatggt gtaccggaat
     4321 tgcttttcat tctttgttca tctgctttga attcacgggg aagaatgttt tcttcctgtg
     4381 agaataaatt tcctgaactt aaacaaaaaa taaaaataac cagtaactca aaaaagtaag
     4441 tggatttttt catgtggtaa ttatttttga ttaataaaaa atatttttga agacccgcgt
     4501 tctgaatttt aggaattgaa cataggtctt cctttaattt ggataaaatt attttgcaaa
     4561 cttatttcta ttttttaaaa aataaaaaca agaattaatc agacttttta aaaatgctaa
     4621 aaaggctgcg ccattaaatt gtaatttatt gtgaagacca gatctcagaa attttggggg
     4681 tttgaaatga tcttcgtatt ttcaaaataa ttaattttga aatattttat tttcgtattt
     4741 agaacaaaaa tttatcagac atatacaaaa tattgaaagt ctgactttgt aattgttttg
     4801 aatttttgaa gacctggatt ctgaagtttt gaaagatgaa tgaaggtctt cctttaaatg
     4861 gaaaaattat aaccggtttt atttttttaa ataaatatta acctgtttgt gaagtttgaa
     4921 aattggattg tattttattt gggttaataa atccggaaat attattttag atcatcccgg
     4981 aaataaccgg cagataaaat aaaaaaaagt acctctttcc atattgattt tcttattaat
     5041 tgatttttaa tttttcaaag ttaataatat atttttttta ttacaacact aaattcattt
     5101 tgggaaaagt ttttggagaa aaaattgtat acgtttattt ttttgcaaat ttcttttttt
     5161 taatattttt tagaaccata ttcatatgac agttttttca gacttaaaaa aaaatttttt
     5221 ttacattccc tctaatccta tacagctaaa gctttgtact gacattctaa aatttttaaa
     5281 tactttttaa tgagcaaaat ttcataaaag agtcttatgc atttttttaa aaatggttta
     5341 aaaataaaag aattaattca tcattgtata caataaaaaa atttaaaaaa atttctgaat
     5401 tttaattaca ttactctcaa aagataaaat taataaattt gcaatgacag tatggagatt
     5461 tcatattgct gaaataattt gaaattattt taaatggaca taaaattttt aaaaggtgtc
     5521 ggtgaaaaaa gagctgaagc attctcaaaa ataggtattg aaacgctgga tgactttctg
     5581 aatttcattc ccagaacgta tctaaaaaaa atcaacatca gagaaattcc atccaacata
     5641 aatgaaaatg ttatcatttc cggaaaggtc attgatcttg aacatcccag aaaaagcaat
     5701 cacccataca gagttgtatt aagagataat acaggaacag cagcaattcc aatctttgga
     5761 gcaagtgaat tccgctcaag acagttccgc ttgaatgacg atttcatatt ttgggtaaag
     5821 atcgaaaagg ataattttgc ttctgttccg aaaatttctt acagagatca tcttaaaatt
     5881 aataccgtag atccgataga tctggatttt acgaaattca gtctgattcc ggtttatgaa
     5941 ctttcaggca ttttaaaaaa gacatggata aaaccgctgc tgctttctaa aatagtattc
     6001 aatgcattca acactatgct caaatcaaat cccgactata tagaggagac actgcctgaa
     6061 gacattgtca gccgttacaa actttccggc aaaaaagaag cagtcttaag aataaatttt
     6121 cctgtagaat tttccgacat tgagttttca cgcaagcgtc tttcatttga agaattattt
     6181 tatctcgaac ttattcttgc attaaaaaag aaatctctcg aaaatgaaaa gaagggaatt
     6241 aaatttaata acgacattca tgtcattact gataaattta aatccgtatt gaaattcgag
     6301 ctcacaaatg cacagaagaa agtgatgaat gagatctatc tcgatatgaa ttccgataag
     6361 tgtatgaacc gtctgctgca aggtgatgtc ggaagcggaa aaacaattgt cgcagtttat
     6421 tctattttga tcgcaatcga aaacggatac caggcagcat tcatgtgccc cactgagctt
     6481 ttagctgagc agcattataa gaccatttct gaattactaa aaccgcttga tataaatgta
     6541 tgccttcttg tcggaggaca aaagaaaaag ctgcgtgcgg aaattctcga aggcattagt
     6601 tcaggaaaga ccaatcttat aatcggaaca catgcgctga tacaggaaac tgttgaatat
     6661 aaaaatatcg gatttgttgt gatcgatgag cagcataaat ttggagtgat gcaaagagcg
     6721 aagctgaaag aaaagggttt caatcctgat gtactggtaa tgactgccac acctattccg
     6781 aggacgcttt ccatgacata ttatggtgat ctcgatgtat ctgtgatcga cgaacttccg
     6841 aagaacagga tccccgtcaa gacagcttta agatccgaat cagacaagcc tgcaatttac
     6901 aaatttgtca gagaagaaat caaaaaggga agacaggtgt acatcatatt tcctattata
     6961 gatgaatcag aaaaactcga tctgaaatct gcagaagaaa attatctcat tttaaaaaat
     7021 gatatttttc ctgatatgaa agtcggtatg gtacacggga gaatgctttg gtatgaaaaa
     7081 gatgaggaga ttgaaaactt ccgtgacagt aagatgaata tccttgtagc tacaactgtg
     7141 atcgaagtcg gcattgacat tccaaatgca actgtaatga taatagaaga agcacagaga
     7201 tacggacttt cgcagcttca tcagcttcgc ggcagagtcg gcagaggtgc agatcagtct
     7261 tattgtattc tgatcgcaaa ggatctttca gaagattcag caaagcggtt aaatatttta
     7321 tgcaaatcaa cagacggatt taaaattgct gaagctgata tggatataag aggaccgggc
     7381 gaattttttg gtatcagaca gtcaggcata ctgaattttt cctgtaccga tctcaaaaaa
     7441 gataaagacg ttctcttaat tgcaagagac attgctttta aaattattga tgaagatcct
     7501 cagcttagaa aaaatgttca cagtgtaatt aaaaagacat ttcttaaaaa ttataaggac
     7561 tcagtttatc ttactgatgt tgcctgaaaa aaacttcgaa aacagcaggg aataatgaat
     7621 attgaatatt gtaatgaaat gttttggacg gatgagaaca tttttatgtt ttgatagaat
     7681 aattacgttt cctattaaag tgaaataaat tattttgtat ctcataaaaa tcaatatcca
     7741 cagtctctga atttaatttg aaaagcagat caactaaaat agaagtaata atccctttac
     7801 tgacagtatt gtcagatgct gtttcaatat ttggtgcatt tctaatctct tactggataa
     7861 ggttttatag tccgctgaca aaaattattc cggtaacaaa aggcatccct gacatcagtg
     7921 gctatattga attctcctta atagtaattc ccatctggct tatcattttt cagaccagaa
     7981 aaatgtatcg tctcaaaaga aacgttttta ttttcgacga attttttgta atcattaaat
     8041 gtatctccat aggcgtcctt attgcgatgg gacttgtttt cattctcaaa ggagatttcc
     8101 cttattcaag acttgtattt tttctcatct ggctattttc cattttcatt gtaactgtcg
     8161 gaagatattt cttgctgaag atcgaaaaga atttatataa taatgataaa ggtgtaaata
     8221 atgtggcaat actcggaaca aatttaatgg caaaaaatat ttatgagaat ttttcaagag
     8281 ataaattcac cggttatcat attaccggat atttttctta tggtgaaaat tcaacaggtg
     8341 aaatggaagg taagaagtat catggtgatt atattttgat acctgatatg ttacaggaat
     8401 taaatctcga taaaatattc atctccattc cttcttccga acatgacata ctttataatc
     8461 tttttaaaat atgcgaagga aaaaatattg aattcatgta tgcacccgat tttattgaca
     8521 ttatgacaag cagactacgg atcgaagaag ttgacggcat cccgttcatg aagctgaaat
     8581 ctctgccaat gaatgtgtgg aacagattta ctaaacggac ttttgatatt gtagtatctt
     8641 ttcttgcatt attattcctt tcacctttaa tgatagtatt atctatttta gtaaaactta
     8701 catccaaagg tccccttttt tataatcagg aaagagtcgg acttgacgaa cagaaattca
     8761 taatgctgaa attcaggtca atgaagatcg atgctgagaa aaaaggaccg cagatgacaa
     8821 ccagagatga cgacagatat actccgattg gaaagatatt aaggaaatac agtctcgatg
     8881 aacttcccca gttttataat gtactgatcg gaaatatgag cattgtaggt ccgagaccgg
     8941 aaagagaatt ttttataaac tctatgaaac attctatcag aagatatctc gaaagacaca
     9001 gggtcaaagg cggaatgaca ggttgggcac aggtcagcgg actcagaggc actgattctt
     9061 ccatgcagac aagaatagat tatgatattt attacattga aaattggtct atagctttcg
     9121 atattaagat tatttttaaa accataaaag aagttctgtt ttctaaagaa gcattttaat
     9181 ttatgatcat tgcagtaatt tattttatac acgtcatttt tgcagtctat gctttttcaa
     9241 aatcttatca gaatgacgga ctccttcagg cttttctgaa tctcggattt atcataactt
     9301 tgtttgctgt ttcacttact gtttgcgaac ttattgtcgg gttatttatt tctgatacag
     9361 gttacaatat gctcttacct gttaattcag ttttactttt ctttttaaaa atatccggct
     9421 tttatgctca gcaggcaaat gtcattactc ttactcccaa agatactatt actcttatac
     9481 ttgttacgat actggaattt tctttctata aattctattt caaaaaaaca gagattacta
     9541 agatcgcctg attcattaat atataattta atttgtcttt taaaaaatta tcattattat
     9601 ttattcttat ttctgctttc gttgcttatt catttagcag caggctgcat aatacagttc
     9661 agaatacttc cagctcaagg gatattgcat tgcagttcat tacttataat ggttatggaa
     9721 ataatcttta tatagacaat gtactcaccg gtgaaagaat atctaatgat gttgcagtta
     9781 cttcaataat aaatttcccc attgattcag tttattctat atatacttca ggaacggata
     9841 ctgtagcgcc cattctctct gtggcaaata tcggattaag taattcatcg gattcgatta
     9901 cagttcatat gaaaattgat ccgggaaatt atctcagaga tactttggta cgtggattag
     9961 ctccgggaca gaccgcattt
//
LOCUS       DAORVQ010000117         9978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3588_length_9978_cov_4.205482, whole genome shotgun sequence.
ACCESSION   DAORVQ010000117 DAORVQ010000000
VERSION     DAORVQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_9978_cov_4.205482"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLX80_09825"
     CDS             <1..577
                     /locus_tag="PLX80_09825"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQY21128.1"
                     /translation="KNYIIAADGGANQLPENDIDPDIIIGDLDSISTKVLNFYKKSKK
                     EIIKISEQETTDFEKALNFCIENGIKELKVFGAVSERPDHTFNNFSILKRYYKSLDVI
                     LYSNEFEIYFINKFTDFKYKKNNTVSLMPFPKAISIKTEGLKYKLSEEDLVLGKREGS
                     LNVSNAERVKISFSKGDLLLFKKHFIHNPKI"
     gene            574..1989
                     /locus_tag="PLX80_09830"
     CDS             574..1989
                     /locus_tag="PLX80_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQY21129.1"
                     /translation="MKIILSTNDYLIIIAYFIIVLIIGFRTKKSDNSVKDYIVGGRML
                     TLPAFVATLVASFYGGVLGIGEFTYTYGISGWLLYAFPFYIFITFFAFFLADKIRKTH
                     LYTIPDQLFKVYGKKVSIIGGVLIFLLATPAPFLFMLGILIKLIFGFNIIYAMIIALL
                     LSVLYLFNGGFRADVRVNIFEFILMFVGFGIILPFCFNTLGGTEFLESRLPESFMSLT
                     GGLSIQLFFVWFFIGSWVLIDPVFHQRCYAVKNKKTPKRGILISLVFWIIFDFLTTAA
                     GLYARAFINIEDIPSPLYSYPALAENILPPIAKGFFFIGMIATVMSSLHSYIFISATT
                     FSKDILSRLKDSTGENNSYNKIGMIISALFAYLLAYLIPSVVEIWYTVGTMIIPALLI
                     SVLSSYFVKFQVHSKYIFLAMISSFSVSFISFIIGNLNSVNGIAVYPFLMEPMYPGLI
                     VGIIIYLIGYKNRKYLKTVTD"
     gene            2080..4821
                     /locus_tag="PLX80_09835"
     CDS             2080..4821
                     /locus_tag="PLX80_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY21130.1"
                     /translation="MKNIQGKEILSFVNLNDSSLTSVNPSDTIKLTFKDKVKADTNYF
                     NIFPKDPNSLKLALVLSGGGARGIAQLGVIRALEEYNVDVDIIVGTSIGTVIGGLYAS
                     GYTTYEIEKIIKDFNWDRALSLTNKYQRTSLFLEQKKIQDRSLLTIPLDGINPDILPS
                     SFSNGQYLSEKINTLILNARYHAKNSFNDLKIPFVAVATDITTGDKVVLKKGNLSESI
                     KASITFPLLYTPININGRKLVDGGLSANIPTDIAKEQGADFTLIVNSTSPLKSIKDLN
                     DPLNTADQILSITMMQLSNLQLKDANAILTPDIKNFSSNSYSNFDYLIRKGYESVVEN
                     IKSTLNAIDSLEIAKSKYFNNFVTNPEIIINTPDDLLPIIDSVLKRSSQNFLKYTEIE
                     KDLKDVYKTGYFEDAYAVISRDNNSVKINYHFIPYPVFRNATINISDLNINKDVENFI
                     IKNSGKYLNVLAADDFRNVLLGNLRDEGYSLKDITKFYFEHNTGILEIKVDDGKINAY
                     KLIGNKVTKDNVILREMKTDISNPIRSKDVNLSLKNVMSTNLFQQISFDYLYEKNNEN
                     PDLLIRVIEKNTKALRFTLKADNEKNLQLLFDLRDENILGSAIEAGILAAGGLRNRIY
                     EIEIKSNQFFSLPLTFNLNGFYIFNDIYTYIQVNDTNENEYSVIRTGEYRNLKTGSSF
                     LFGTQLERYGTFYIQGIAENLEIINKSNNEDKSSNANVFKLRLGGVIDTEDKIPFPNE
                     GVLLNFYYETAKNINEGNQSYTKLFFNYDQFIPLSAVSTIKPRFTFGFGDKTTPLSEQ
                     FSMGGQKNFFGMVENELVGRQILIASAEYRYEFPYRLFFDTYISIRYDLGNVWENTTD
                     IRFKDLRHGLGLSASFDTPIGEAVFSVGRSFQITSGFNKNSFIFGPYDFYYTLGFDI"
     gene            4897..7419
                     /locus_tag="PLX80_09840"
     CDS             4897..7419
                     /locus_tag="PLX80_09840"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQY21131.1"
                     /translation="MKFLNRFSDGTLRFVFTFIAIYCIAYSAIVFVNTMFTDRMTTDD
                     CLWVNENVNKELKEGLLIVQVMKDGVTDKAGIKDGDVLIALDGQKFKGSQDAQAILNS
                     YEPNQLVEYTVIRDGKTIITNVKVYKVFNFLYLIFSLLGLGFILISFMVGYSKPKELT
                     SQIFFFLGCSASLGFNLIGLNNSFIFSNKFYLNSVIGLTLFYPLFVHFFLTYPVKYEF
                     KNRKYLVFSLYLIVLIFAIINSMSVSRGQYSVFISTFQFIVIASYFTAGISLFFVSYY
                     KLEKDKQRKPLKVIFYGFIFGLIGFAYTGLISIFIQKPNFLIDTWIYLPTILVLAVPL
                     SFGFSIIKYRILDTEFIIKKGLVFGIITAFIVGIYLLLVLFLDNILSQLLPENKQLLT
                     IALIIIVTFSFDFVNKKAKDLVDKQFYRERYNYRKSLLLFSEELPYLTNIRQIIEKIA
                     LSVKETMGINNLYVWFLDDDYYKSMESEIESFEGKSQVFNNEEIYDEAFSFLFKLNKE
                     PKFLSEVYLQEMNVPEEYREIIKKENFILSVPIYIKDKLIGAINFGEKPSGKAYSEED
                     IDLLKTLASQAAIAFENSRLQEEKISKQLIEEELQIARKIQMGLLPQTINSVSGIEVS
                     GFYNPAKIIGGDFYDVIKISETKMLVVVADVSGKGIPAALYMSKVQAMIQFAAKIFPT
                     PKEILVEVNKQIHQKIDKKSFITTVIAMFDLEKMTVNICRAGHNPVIYSVNGKFELLK
                     NKGMGLGLESELYFDKNLEESEIKIAPDNMFVFYSDGLTEAMNISREEFGTDKMLDII
                     QFNRNSNCDLIQDKIIASVANFRGNAEQNDDITLVITKILKK"
     gene            7491..8783
                     /gene="purB"
                     /locus_tag="PLX80_09845"
     CDS             7491..8783
                     /gene="purB"
                     /locus_tag="PLX80_09845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQY21132.1"
                     /translation="MIPRYSRKEMSDIWSDENKFRIWLDIEILATEAQSKLGNVPVSS
                     LKNIKKNAKFNTAKILKIEEEVKHDVIAFLTNVAESTGMDSRFIHMGMTSSDVIDTAL
                     SVQMKQAGEIIMNGLISVKKSLAVVAKKYKYLTMIGRTHGVHAEPITLGLKFALWFDE
                     MNRNIERWKRAVEVISVGQISGAVGTYEHISPKVEQFVCRQLGLKTTKISTQILQRDR
                     HAEYMSVLAIIASSIEKFTSEIRHLQKTETLEMEEYFSSGQKGSSAMPHKKNPITCER
                     LSGMARIFRGNAVAAFENIPLWHERDISHSSVERVIIPDSTILMDYMLYQFKKLIDTL
                     VINKDNIRKNLELTNGLVFSQTVLLKLIDKKMKREQAYKIVQTVAMKCWKEKKSFLEL
                     LKEDKEVLKYLNDKELGEIFNYDKSRKSVDFIFKRTGL"
     gene            complement(8974..9603)
                     /gene="lexA"
                     /locus_tag="PLX80_09850"
     CDS             complement(8974..9603)
                     /gene="lexA"
                     /locus_tag="PLX80_09850"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQY21133.1"
                     /translation="MHKLTAKQKKILEFIEKFGNEYSYPPTLKEIGEKFNITIGTVQD
                     HIKALQSKGWLERRKDIARGFVVVKSEEENNIERLRSEVNIIPVYGHVAAGEPIFADD
                     NVQGYITMEKNIKGHKIHFAVKVKGDSMIDSGIYDGDIVIVRKQDTAEDGEIVIALLE
                     DEATVKTLRNKRIKAYLEASNSNYKSIINKPFKIIGKVIELRREYNLIN"
BASE COUNT         3392 a         1511 c         1686 g         3389 t
ORIGIN      
        1 aaaaaactat ataattgctg ctgacggagg cgcaaatcaa cttcctgaaa atgacataga
       61 ccctgatata attataggtg atcttgactc tatcagcaca aaagttttaa atttttacaa
      121 gaaaagtaag aaagagatca ttaaaatttc cgaacaggaa accactgatt tcgaaaaagc
      181 cttaaacttt tgtatagaaa atgggatcaa agagttaaaa gtttttgggg ctgtaagtga
      241 aaggccggat catactttta ataatttcag tatattaaaa aggtattata aatctcttga
      301 tgtaattcta tattcaaatg aatttgaaat ctattttata aataagttca cagatttcaa
      361 atataaaaaa aataatactg tttctttaat gccattccca aaagcaattt caataaaaac
      421 agaaggactt aaatataaac tatcggaaga agatcttgta ttgggaaagc gggaaggttc
      481 acttaatgta tcgaatgcag aaagagtaaa gattagtttt agtaaaggag atcttttatt
      541 atttaaaaaa cattttattc acaacccaaa gatttgaaaa taatattatc tacaaatgat
      601 tatctgataa tcatcgctta ttttataatt gtacttataa ttggattcag aacaaaaaag
      661 tctgataatt ctgtaaagga ttatattgta ggcggcagaa tgttaacatt gcccgcattt
      721 gttgcaacac tcgtagcctc tttttatgga ggtgtactgg gaattggaga attcacatat
      781 acatatggaa tatcaggctg gcttctatat gcatttccat tttatatatt tataacattc
      841 tttgcattct ttctcgcaga taaaatcaga aaaactcatt tatataccat ccctgatcag
      901 ctgttcaaag tctacggaaa aaaagtaagt atcataggag gagtattgat ctttctgctt
      961 gcaactccag cacctttttt attcatgctt ggaattctga tcaaactaat tttcggtttt
     1021 aatataattt atgcaatgat catcgctctt ctactttctg ttctgtattt attcaatggt
     1081 ggtttccgag ctgatgtacg ggttaatatt tttgaattca tattaatgtt tgtaggattt
     1141 ggaattatac ttccgttttg ttttaatact ctcggaggaa cggagttttt agaatcaaga
     1201 cttcctgaga gttttatgtc tcttaccggc ggtttatcga tccagttatt ttttgtctgg
     1261 ttttttatcg gatcgtgggt attgatcgat cctgtatttc atcaaagatg ttatgctgta
     1321 aagaataaaa aaactcctaa acgcggaatt ctgatctctc ttgtattctg gatcattttt
     1381 gattttttga caacagcagc cggtttatat gcccgtgcat ttataaatat tgaagacatt
     1441 cccagtcctt tatattccta tccggcgctt gctgaaaata tattacctcc aatagctaaa
     1501 ggatttttct ttataggaat gattgcaaca gtaatgtctt ctttacattc atatattttt
     1561 atttcggcaa caactttcag taaagatatt ttatcaagac ttaaggacag tacgggtgaa
     1621 aataattcat ataataaaat agggatgata ataagcgctt tgtttgctta tctattagct
     1681 tatctgattc cttcagtggt tgagatctgg tatactgttg gaacgatgat aatccctgca
     1741 ctgctgataa gtgtattaag ctcttacttt gttaaatttc aggttcactc aaaatatatt
     1801 tttttagcta tgataagttc attcagtgtt tccttcattt cttttattat cggaaattta
     1861 aattccgtaa atggtattgc tgtttatcca tttttaatgg aaccaatgta tccgggtctt
     1921 attgtcggaa taattatata tttgatcggt tataaaaaca gaaagtattt aaaaaccgtt
     1981 actgattaaa tcacttttat cattttcgga tatttgattt tcaaaagact tatgataaaa
     2041 atctgtttct taattctgct ttttttattt catttacctt tgaaaaatat acaaggtaaa
     2101 gaaatattat catttgtaaa tttaaatgat tcatctctga catcggttaa tccttctgat
     2161 actatcaagt taacttttaa agataaagtt aaagccgata caaattattt caacattttt
     2221 cctaaagatc ccaattcact taaacttgca ctggttttaa gcggaggtgg tgcaagagga
     2281 atcgcacaac ttggagtgat cagagctttg gaagaatata atgtggacgt ggatattatt
     2341 gttggtacaa gtatcggaac tgttattggc gggttgtatg cttccggcta tacgacttat
     2401 gaaatagaaa aaattataaa agattttaac tgggacagag cattgagtct taccaataaa
     2461 tatcaaagga cttcactttt ccttgagcag aaaaaaattc aggacagaag tttacttact
     2521 attcctttag acggtataaa tcccgatatt cttccttcat cattttcaaa tggtcagtat
     2581 ctgagtgaaa aaataaatac actgatctta aacgcccgtt atcatgctaa gaacagtttc
     2641 aatgatctga agataccctt tgtagctgtt gcaacagaca ttacaacagg agataaagta
     2701 gttttgaaaa agggtaattt atcggaatca ataaaagctt caataacatt tccattatta
     2761 tatacaccaa ttaatattaa cggtagaaaa ttagttgacg gaggtctttc tgcaaatatt
     2821 ccaacagata tagctaaaga acagggagct gatttcactc tgattgtaaa ttctacaagt
     2881 ccattaaaat caattaaaga tcttaacgac cctttgaata ctgctgatca gatactttcg
     2941 atcactatga tgcaattgag taatctgcag cttaaagatg caaatgcaat acttactcct
     3001 gacattaaaa atttttcctc aaattcatat tctaattttg attacctgat cagaaaagga
     3061 tatgaatcag tagtcgaaaa tattaagtca actctaaacg caattgattc tcttgaaatt
     3121 gcaaaatcaa aatactttaa caactttgtt accaatcctg aaataataat taatactcct
     3181 gatgatctgt tacctattat agattcagta ttgaaaagat cttcacaaaa ttttttaaag
     3241 tacactgaaa tagaaaaaga tctaaaagat gtttataaga caggatattt tgaagatgct
     3301 tatgctgtca tttcacgtga taataattct gtaaaaatta attatcattt cataccttat
     3361 cctgtattta gaaatgcaac aataaatata tccgatctga atattaacaa agatgttgaa
     3421 aatttcataa ttaaaaattc aggtaaatat ctaaatgtac ttgctgcaga tgacttcagg
     3481 aatgtcctgc tcggaaatct gcgggatgaa gggtattcct taaaagatat tacaaaattt
     3541 tattttgaac ataatacagg aattcttgag ataaaagtag atgatggaaa aattaatgca
     3601 tacaaactta taggtaacaa agtaactaaa gacaatgtga tactcaggga aatgaagacg
     3661 gatatatcaa atcctatcag gagtaaagat gtaaatctca gtcttaaaaa tgtaatgagt
     3721 acgaatcttt ttcagcagat aagttttgat tatttgtacg aaaagaataa tgaaaatccg
     3781 gatctgttga tcagggttat tgaaaaaaat acaaaagctc tgagatttac tctgaaagct
     3841 gataatgaaa aaaatttaca gttattattt gatttgagag atgagaatat attaggcagt
     3901 gcgatcgaag cgggtattct ggcagcagga ggtctaagga acaggatcta tgaaattgaa
     3961 attaaatcaa atcagttttt ttcactgcca cttactttta atctcaatgg tttttatata
     4021 ttcaatgaca tttacacata tattcaggtt aatgatacaa atgaaaatga gtattcagtt
     4081 ataaggacag gtgaatatcg taatttaaaa accggttcaa gttttctttt tggaacacaa
     4141 cttgaaagat atggtacttt ttatatacag ggaattgctg aaaatcttga aattataaat
     4201 aaatctaata atgaagataa atcttcaaat gcaaatgttt ttaaattaag acttggcggt
     4261 gtaattgata ctgaagataa aatacctttt ccaaacgaag gagtcctgtt gaatttttat
     4321 tatgaaactg caaaaaatat taatgaaggt aaccaaagct acacaaaact tttttttaat
     4381 tatgatcagt ttattccatt gtcagcagtt agtactataa aacccagatt tacttttggc
     4441 tttggtgaca agactactcc gctttcagaa caattttcca tgggagggca aaagaacttt
     4501 tttggaatgg tagaaaatga acttgttgga agacagatac ttattgcttc tgctgaatac
     4561 agatatgagt ttccatacag attgtttttt gatacttaca tcagtataag atacgacctc
     4621 ggaaatgtat gggaaaatac tactgatatc agatttaaag atctcagaca tggattagga
     4681 ctttcagctt cttttgatac tccaatagga gaagcagtat tttctgttgg aagaagtttt
     4741 cagatcacca gtggttttaa taagaactca tttatttttg gtccttatga tttttattat
     4801 acattgggtt ttgatattta atatttttca aagtaactta taaggttttt tttgcgtaaa
     4861 tttgggttat ctaattttca ttaaccaata tttgaattga agtttttaaa ccggttcagt
     4921 gacggcacct taagattcgt ctttaccttt attgcaattt actgcatagc gtattcagca
     4981 atcgtatttg taaatacaat gtttacagac aggatgacta cggatgattg tctttgggtt
     5041 aatgaaaatg taaataaaga attgaaagaa ggtctgctca ttgttcaggt aatgaaagac
     5101 ggtgtaactg acaaagctgg tattaaagac ggagatgtcc ttatagcatt agatggtcaa
     5161 aaattcaaag gaagtcagga tgcacaggcg attcttaaca gttacgaacc taatcagctg
     5221 gtagaatata ctgtgataag agatggtaag acaataatca caaatgtaaa agtttacaaa
     5281 gtgtttaatt ttctttatct catattttca ctcttaggtc tgggttttat actaataagc
     5341 tttatggtag gatattcaaa accaaaagag ctcacatcac aaatattttt ctttcttgga
     5401 tgctcagcat ctctcggctt taatcttatt ggcttaaata attcatttat attcagtaat
     5461 aaattttatc tcaattcagt aattggtctg actctgtttt atcctctctt tgtacatttc
     5521 ttcctaactt atcctgtaaa atatgaattc aagaacagga aatatttggt attttcatta
     5581 tatctgatag ttttaatttt tgccataatt aattccatgt cagtatcaag aggtcaatac
     5641 agtgttttca tatcgacttt tcaatttata gtaatcgcaa gttatttcac agcaggtatt
     5701 tcattatttt ttgtttcata ttataagctg gaaaaagata aacaacgcaa gccattaaaa
     5761 gtaattttct atggattcat attcggatta atcggatttg catataccgg tttgatatct
     5821 atatttatac aaaaaccaaa ttttcttatt gatacgtgga tctatcttcc tacaatatta
     5881 gtcttggcag ttccactttc atttggattc tcaataataa aatacagaat actcgataca
     5941 gaattcatta ttaaaaaagg actggtgttt ggtatcatta cagctttcat tgttggaatt
     6001 tatttacttc ttgtcctttt tctcgataat attctcagtc agcttcttcc cgagaataaa
     6061 cagcttctta caattgcctt aataataatt gtaactttca gttttgattt tgtaaataaa
     6121 aaagcaaaag atctggttga caaacagttt tacagagaga gatataatta cagaaaatca
     6181 ctacttctgt tttcagaaga acttccatac ctgactaaca taagacagat cattgagaag
     6241 attgctcttt cagtaaaaga aactatgggg ataaataatc tttacgtctg gtttctggat
     6301 gatgattatt ataaatctat ggaatcggaa attgaatcct ttgaagggaa aagtcaggtc
     6361 ttcaataatg aagagattta tgatgaagca ttctcatttc tttttaaact gaataaagag
     6421 ccaaaatttt taagtgaggt ttatcttcag gaaatgaatg tacctgaaga atacagagaa
     6481 attataaaaa aagagaattt cattttatct gttccaatat atattaaaga taagctgatt
     6541 ggcgcaataa atttcggaga gaaaccgtcc ggcaaagctt attccgaaga agacattgat
     6601 cttctaaaga ctcttgcatc tcaggcagca atagcttttg aaaactcaag actgcaggaa
     6661 gaaaagattt ctaaacagct aatagaagaa gaacttcaga tcgccagaaa gatccagatg
     6721 ggactacttc cccagactat taacagtgta agcggaattg aagtatccgg attttataat
     6781 cctgctaaga ttatcggtgg tgatttttat gatgtaatta aaatcagcga aacaaaaatg
     6841 cttgttgtag tcgcagacgt ttcaggaaaa ggaattcctg ctgcgctcta catgtctaaa
     6901 gtacaggcaa tgatacaatt cgcagctaag atctttccaa ctccaaaaga aattttggtt
     6961 gaagtaaata aacagataca tcagaaaatt gacaagaaat catttatcac gactgtgatc
     7021 gcaatgtttg atctggagaa aatgactgtt aacatctgca gagccggaca taaccctgtg
     7081 atatattctg ttaacggaaa attcgaatta cttaaaaata aaggtatggg actcggtctt
     7141 gagagtgaat tatattttga taaaaacctt gaagagtcgg agatcaaaat agctcctgat
     7201 aatatgtttg tcttttattc agatggtctg accgaagcaa tgaatataag ccgggaagaa
     7261 ttcggaacag ataaaatgct ggatattatt cagtttaaca ggaacagtaa ttgcgatctc
     7321 atacaggata agatcatagc ttcagttgct aacttcagag gcaatgctga acagaatgat
     7381 gacattactt tagtaattac aaagatcctg aaaaagtaac tttatataaa actcaccatc
     7441 aattattttt ggtcaaaaat tttttattcc tcatataatt caaattaata atgataccaa
     7501 gatattcccg taaagaaatg tcagatatat ggtctgatga aaataaattc agaatttggc
     7561 tggatattga aatacttgca actgaagctc aatcaaaatt aggaaatgtt ccggtaagtt
     7621 cattgaagaa tattaaaaag aatgcaaaat ttaacactgc taaaattctt aagatcgagg
     7681 aagaagttaa gcatgatgtg attgcctttc ttacaaatgt agctgaatca acgggaatgg
     7741 attccagatt cattcacatg ggaatgacaa gctctgacgt gattgacact gccttatctg
     7801 tacagatgaa acaggccgga gaaataatta tgaacggact tatctctgta aaaaaatcac
     7861 ttgctgtagt tgcaaaaaaa tataaatatc ttacgatgat cggcagaaca catggtgtac
     7921 atgccgaacc gatcacactg ggtttaaaat ttgctttatg gtttgacgag atgaacagaa
     7981 acattgaacg ctggaaaaga gcagttgaag ttatttcggt cgggcaaatt tccggagcag
     8041 tcgggactta tgaacacatt tctccgaagg tagaacaatt tgtttgcaga cagcttggct
     8101 tgaagactac taaaatttca actcagatat tacagaggga cagacatgct gaatatatgt
     8161 cagtacttgc tatcattgct tcgtcaatcg agaaatttac aagtgagatc aggcatttac
     8221 aaaagactga gacgcttgag atggaggaat atttttcatc cggtcagaaa ggttcctcag
     8281 caatgcctca taaaaaaaat ccaatcacat gcgaacggct ttccggaatg gcgaggatat
     8341 tccgcggaaa tgccgttgct gcatttgaga atataccgct ttggcatgaa agagatatta
     8401 gtcattcatc tgtcgaaaga gtgataatcc ccgactcgac aattcttatg gattatatgc
     8461 tttatcagtt taaaaaactt atcgatactc ttgttatcaa taaagataat atcagaaaga
     8521 atctggaact tacaaatgga ttggtatttt cgcagactgt tttactgaaa cttattgata
     8581 aaaaaatgaa aagagaacag gcgtataaga tcgttcagac ggtggcaatg aagtgctgga
     8641 aggaaaagaa gagttttctg gaattactga aagaagataa agaagtttta aagtatctga
     8701 atgataaaga gcttggagag atttttaatt atgataaatc gaggaagagc gttgatttca
     8761 tttttaagag aacagggctt taaaatatta aattgtaaat tttaaatatt aaatttttaa
     8821 cagataactg aagttaagca attgtcggta tttactaaat accagctggt gcttgtttcc
     8881 aagctctatt tggaaatgca gttttcaagc gaaacaccgt ttcgcaatag ctctttcaat
     8941 agcaatcagc aataatttta aaattgcata actttaattt ataagattat attctcttct
     9001 cagctcgatc accttaccaa taattttaaa aggcttatta attatagatt tatagttgga
     9061 atttgaagct tcgagataag ccttgatcct tttatttcta agtgtcttta ctgtagcttc
     9121 atcttcaaga agagcaatca caatttcacc gtcttcggct gtatcctgtt ttctgacgat
     9181 cactatatcg ccgtcatata ttcccgaatc aatcatactg tcccctttta cttttacggc
     9241 gaagtgaatt ttatgtcctt taatattttt ctccatcgtg atatatccct gaacattatc
     9301 atctgcaaat atcggttcac ctgcagcgac gtgaccataa acaggaataa tatttacttc
     9361 tgatctgagt ctttcgatat tgttttcttc ttcggatttt acgactacaa atccgcgggc
     9421 aatgtctttt cttctttcga gccatccttt actttgaagg gctttgatgt gatcctgaac
     9481 tgttccgata gtaatgttaa atttctcacc gatctctttt aatgtaggag ggtaagaata
     9541 ttcattaccg aatttctcaa tgaactcaag gatctttttc tgtttggcag ttaatttatg
     9601 catagataaa tatggcataa ttatttaata gtgtcaatag aaaattttga ccttgattcg
     9661 catggtttta ggattacaag attttaattt acatttttta caaacacgaa ttcctgtaaa
     9721 tccggatctg gcaaccataa ttccggttat tttcttacaa tatttttttg aaccatctcc
     9781 gccagccctt taaagacagg ccttagaacc ccgtccattt tattttttca caaaccgtct
     9841 tttttaccat ttgactcgtc tgcatatttt tacaatattt gtgagaattt atgattatgt
     9901 attgatttta tgatcagctt caattttact ttctgatttt atttacggaa gaatttttga
     9961 gatgaacttg ttgaaaat
//
LOCUS       DAORVQ010000118         9974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3593_length_9974_cov_4.557718, whole genome shotgun sequence.
ACCESSION   DAORVQ010000118 DAORVQ010000000
VERSION     DAORVQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3593_length_9974_cov_4.557718"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..3136)
                     /locus_tag="PLX80_09855"
     CDS             complement(38..3136)
                     /locus_tag="PLX80_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQY21134.1"
                     /translation="MNSYSEILFSGLNIENGLSNSTVKTILQDYLGFMWFGTQDGLNR
                     YDGYDFKIFRSDSEKSCHLSNNIVNVIFEDSSGVLWIGTDDGLNIYDRKMDNMKRVGI
                     SNFTSGINSETIRTIYEDNEKNLWIGTYGGGLICLDPERNLINCFRSTADNESSISSD
                     RVSSVIQDKQGNIWAGTWGKGLNIFNKRENKFLRFINDPDDVFSLSENKVDSMFCDRA
                     GVIWAGTTNGLNRYDNELNRFMRFNHDPEDRYSLSNRLLSNICEDKHGNFWIGTKEGG
                     LNYFDKAENKFYNYKDEKNNPYSIGNNSILNVYSDRSGILWVGTYGSGLRIYKNQTKK
                     FHHYFEKPSDSNSINSNKISCFAEDKRTGNLYIGTADKGITIYLKESNKFIRLKENMS
                     DSHQNMTDCMIQHSDGNIYIGTSGSGLVKFDHVSEEYISYRFKIKGDDTKNYLYVFSI
                     HQDDDGYIWFGTAGKGVNRFDPEKEETFHYSIYPDNQNDRRDKRIRKIICKDKNTLIL
                     GSSFGGLLFFDKVKKKFSQYKNDPKDPKSISGNFITDILKDSKNNIWVGTIENGLNKM
                     THGKKGFKRYSTNEGLPNNCISGILEDDDGYIWISSNKGISKLNPESGSIKNYDVRDG
                     LQSNEFNPWACLKLKSGKLVFGGINGFNIFDPSVIKDNLNSPEIVFTDFKIFNKEVKF
                     GKNESILKSTITLTDEINLTYKESVFSFEFSALDFNIPGKNLYAYKMKGFDKDWINSG
                     TRRFATYTNLDAGEYEFTVIGSNNDGIWNKKGKSIKINISPPFWKTIWFKSLSAVSVL
                     AASGSIYQSKINRLRRERKAQEAFTKKLLDTQENERKRIAGELHDSIGQDLLISKNKL
                     QMSLNNSSDIKVVIKNINEVTEVITDTLKNVREISYSLHPYQIERLGLSKAISSIIDR
                     LKGTVEIKFTINIDNIDKTLYPESEISIYRIIQECMNNIIKHSKAKEVILNVSREENY
                     ISILIDDDGVGFSNEKLIAEKGKHGFGIQGMKERIRLINGEMNINSNPGEGTSLNFKI
                     PI"
     gene            complement(3359..5140)
                     /gene="aspS"
                     /locus_tag="PLX80_09860"
     CDS             complement(3359..5140)
                     /gene="aspS"
                     /locus_tag="PLX80_09860"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQY21135.1"
                     /translation="MNFRKRTHNCGELNLKNENEIITLNGWVSKKRDLGGLLFIDLRD
                     RYGITQLKAIPENKEIYDTALKLGQEFVISATGKVLKRESINKNIPTGEIEIELTDLE
                     ILNESDIPPFVVEDDIKASDELRLKYRYLDLRRNSLTDNFVVRSKASNIIHKYFEKHG
                     FLEVETPMLMKSTPEGARDYLVPSRVHNGKFYALPQSPQIYKQILMVSGFDKYVQICK
                     CFRDEDLRADRQPEFTQIDLEMSFVDQDDVFEILEGMFGEMWKEIKGIDLPEKFRRMT
                     YDEAMNSYGSDKPDLRVEGEMKIENISEIVKVSEFKVFSDAIDNGGIAAGIKLSSKEV
                     SRKIIDGLTEFVKKFGFGGLGYLKFNEDGSVQSPIVKFLNDEIIENIKKKFEAEKGDT
                     IFILSGERMKVLQSLGQLRLRLANEFALTDESRFEFVWVIDFPLFRYEEEDKRFYAEH
                     HVFTMPKDDYIKYLDSKEINEIESIRANCYDLVLNGHEITSGSIRIHKPEIQKKVFSI
                     IGLTDEEAKMKFGFILEAFKYGAPPHGGAAVGFDRVIAILCGLKSIIDTIAFPKTLKA
                     SSLMDECPGEVSQEQLNELGIELKIKK"
     gene            complement(5315..6460)
                     /gene="hppD"
                     /locus_tag="PLX80_09865"
     CDS             complement(5315..6460)
                     /gene="hppD"
                     /locus_tag="PLX80_09865"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HQY21136.1"
                     /translation="METINETEVKVQDADTAKDFLPLKRIDHLEFYCGNAKQSTFFYQ
                     YVMGFKLTGYSGLETGNREKTSYLLEQGKIKFVLSSPLTDDNFIAEHHKRHGDGVRDI
                     AIEVDDATKSYFETTSRGAKSVLEPTEFKDENGVLIRSAIQTYGDTIHSFVERKNYNG
                     LFMPGFVSAEHRSVAGIKDTGLKAVDHIVGNVELGTMNDWVKFYAHTMGFNQLISFDD
                     KDISTDYTALMSKVMQNGTGKIKFPINEPAAGKKKSQIEEYLDFYKSAGAQHIAMSTA
                     NILETVNELKSRGLEFLTIPTTYYKELVERVGKIDEDISDLEKLGILVDRDEDGYLLQ
                     IFSKPVEDRPTVFFEIIQRKGARSFGKGNFKALFEAIEREQDLRGTL"
     gene            complement(6614..8671)
                     /locus_tag="PLX80_09870"
     CDS             complement(6614..8671)
                     /locus_tag="PLX80_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQY21137.1"
                     /translation="MKKIKYFTFGVLYFLISFSFLSFQNSFADQLSLNSISGINSSTY
                     TFSDFPRTYQLYARDTQDSAFVTYSGTINVTGYDSVYMEVYRNSTLWKRRSAKLVYAN
                     GKSWFDLKQKIHAELTEFNFKFYLRSGSSSTLISIANNVVCGDFFMIAGQSNSTPTDA
                     LMTYENEFCRSFGMQTLNYNGFPYNPVDTLWGLSKADGAVRVFSGPFNVGIWGLELQK
                     LIKENFGIPTCIINGGGAASTIELNLRNNSNHTDFNTLYGRQLYRTKKAGLTDKVKAI
                     FWFQGESNGTISWLNYQQNFQTLYNQWKENYPGFDFTYVFQTRPCCSAQYASQLREVQ
                     RKLPESFPDLEVIATAGLPYYGGCHYQYNGYKNIGTVVFKYISEKFYTPVDTTGKIGP
                     NIRTAFYTNSQKNEIALLFNNSTVSYWPADSLGNSMKNYFYLNGQTGLISSGQKSGDT
                     IKLKLTNPSSATKLTYLPTVWTHIDSAVYLGPFLRNNRGIGAYSFHEFPITDNPLTYI
                     NIKAISEGNFNTTYNRLNLSDTVSVYIRNNFYPFQIKDSGKVIFDSLTFTGNFSTSKL
                     SSGNYYIVLKGKNSIETWSNNGGVSVSAGKIINYDFTTSQSQAYGNNMKLINQLYSIF
                     SGDVNMDGYIDLFDMYKIENDAFNLLTGFSDTDINRDGLVDISDLEISDNNVSNFISK
                     ITP"
     gene            complement(9035..>9974)
                     /locus_tag="PLX80_09875"
     CDS             complement(9035..>9974)
                     /locus_tag="PLX80_09875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQY21138.1"
                     /translation="ASKKTGLFKLLKNFTIAFLTKLSPVIRIFYSLAVIAFLFAWVNG
                     NWKLAILTFIIVNFILIYEIAEKLTSGDELKIARDVQTSLIPSNPPEDNYFDISYYYE
                     TAKIVGGDFIDFIKKSDEIYFISTGDISGKGMSAALYMIQVRLLIRQLTDIYDNPCCI
                     LKGLNKNVFRHIKKGLYFSSILVEVNKRNLKICRAGHTPLLMYDSFAGECREIQQKGM
                     AVGLTDGEMFDNSLEEININANPGDVFCFYSDGLTESMNSQKEEFGLENVKKILSENF
                     FRPAEEIKEIYLNEINKFRGYAEVHDDITIIVMKTR"
BASE COUNT         3037 a         1846 c         1357 g         3734 t
ORIGIN      
        1 gtttgtagaa atgactgatt tttaattctt aattatttta aatcggtatt ttaaaattca
       61 aagatgtacc ttctcccgga tttgaattaa tattcatttc tccgtttata agtctgatcc
      121 tttccttcat tccctgaata ccaaatccat gtttaccttt ttccgcaatt aatttttcat
      181 tactgaatcc aaccccgtca tcatcgataa gaatggaaat ataattttct tctctgctta
      241 cattcaggat cacttccttt gctttagaat gttttattat attattcata cattcctgaa
      301 taattctata aatgctaatc tcagattccg gatacaaagt tttgtcaata ttatcaatat
      361 taattgtaaa tttaatttca accgttcctt ttagtctgtc aataatagaa cttatcgctt
      421 ttgataatcc aagtctctcg atctgataag gatgaagtga atatgaaatt tctcttacat
      481 ttttcaatgt atcagttatg acctctgtta cttcattaat attttttatt actactttaa
      541 tgtcagagga attattcaga ctcatctgca atttattttt tgaaattaaa agatcctgtc
      601 caatactatc atgcagctca ccggcaattc tctttctttc attttcctga gtatccagaa
      661 gttttttcgt aaatgcctcc tgagcttttc tctctcttct aagtctgttt atcttacttt
      721 gatatatact tccggaagca gctaataccg aaactgcact taatgattta aaccatattg
      781 ttttccagaa tggaggtgaa atatttattt taatggactt tcctttttta ttccatatgc
      841 catcattatt tgatccgatg actgtaaatt catattctcc tgcatctaga ttggtataag
      901 ttgcaaatct cctggttccc gaatttatcc aatccttgtc aaaacctttc atcttgtaag
      961 cataaagatt tttgccgggt atattaaaat ccagtgcaga aaattcgaat gaaaatacac
     1021 tctctttata tgtcagattt atttcatctg ttaacgtaat tgtactcttc agaatacttt
     1081 cgttttttcc aaattttact tccttattaa atatcttaaa atcagtgaag acaatttccg
     1141 gactgttcag attatctttt attacagacg gatcaaaaat attaaatccg ttaattcctc
     1201 cgaatactaa cttaccgctt tttaatttta aacaagccca tggattgaat tcattgctct
     1261 gaagtccatc tcttacatca taatttttta tacttccact ttcagggttt aattttgaaa
     1321 ttcctttatt actactgatc cagatatatc catcatcatc ttcaagaatt ccactaatac
     1381 agttatttgg taaaccctca tttgttgaat atcttttaaa acccttctta ccgtgagtca
     1441 ttttatttaa tccattttca attgtcccga cccagatatt atttttactg tcctttagaa
     1501 tatccgtaat gaaatttccg ctgatactct tcggatcttt cggatcattt ttgtattgag
     1561 agaatttctt ttttactttg tcaaaaaata acagcccgcc gaatgaagag cccagtatca
     1621 aagtattttt atctttacat ataattttcc ttatcctttt gtctctcctg tcattttgat
     1681 tatccggata tatactgtaa tggaatgttt cctctttttc cggatcaaat ctgttaacac
     1741 ctttccctgc tgttccaaac catatatatc catcatcatc ctgatgaatt gagaatacat
     1801 acaaataatt ttttgtatca tctcctttta ttttaaatct gtatgatatg tattcttcag
     1861 aaacatgatc aaactttaca agtcctgatc ctgaagttcc tatataaatg ttgccgtcag
     1921 aatgttggat catgcagtca gtcatatttt gatgagagtc actcatattt tcctttaatc
     1981 tgataaattt atttgactct tttaaatata ttgtaatgcc tttatctgct gttccgatat
     2041 aaagatttcc cgttcttttg tcttctgcaa aacaggatat tttattactg ttaatactgt
     2101 tgctgtcaga tggtttttca aaataatggt ggaatttttt agtttgattt ttgtaaattc
     2161 tcagacctga tccgtatgtc cctacccata aaattcccga tctgtctgaa tagacattca
     2221 gaatggaatt gtttcctata ctgtatggat tatttttttc atctttatag ttatagaatt
     2281 tattttcggc tttatcaaaa taattcagtc cgccttcctt tgtaccgatc cagaaatttc
     2341 catgtttgtc ctcgcaaata tttgaaagca gcctgttgct cagtgaatat ctgtcctcag
     2401 gatcatgatt aaaacgcata aatctattta actcattgtc atatctattc agaccatttg
     2461 ttgtacctgc ccagataaca ccggctctgt cgcaaaacat tgaatctact ttattttcag
     2521 aaagactgaa tacgtcatca ggatcgttaa taaatctcag aaatttgttt tctcttttat
     2581 taaaaatatt taaaccttta ccccatgttc ccgcccagat attaccttgc ttatcctgaa
     2641 tgaccgaaga gaccctgtcg ctgcttattg aagattcatt atctgcagtg gatctgaaac
     2701 aattaattaa atttctttca ggatccagac atataagtcc cccgccatat gtaccaatcc
     2761 agagattttt ttcattatct tcatatattg ttcttattgt ttctgaattt attcctgaag
     2821 taaaattaga gatcccaacc cttttcatat tatccatttt cctgtcataa atatttaaac
     2881 cgtcatcagt gccaatccat aaaactcccg aactgtcttc aaatattaca tttacaatat
     2941 tattactgag atgacagctt ttttcagagt cactcctgaa aattttgaaa tcataaccgt
     3001 catatctgtt gagtccgtcc tgagtgccaa accacatgaa tccaagatag tcctgcagaa
     3061 ttgtcttcac tgtgctgttg gaaagcccgt tctctatatt caaacctgaa aataagattt
     3121 cggaatagga attcatatta ctttataatt ttaaaaaatt taaaattaaa aaaattatat
     3181 taatgtaaat tagaataatg ctttgaaatt tttatagctg atcagataga atatatttta
     3241 ttttatctga tctgtatttt acgttcagtt gtttttcttt tgtgagtatc tttgtaaata
     3301 tttccttaat ttacttttgc tctttaatat atatatatta ctttttaaaa acataaatct
     3361 atttttttat ttttaattca atcccaagtt cattaagctg ttcctgactc acttcgcccg
     3421 gacattcatc catcagcgaa gatgccttta aagtttttgg aaaagcaatt gtatcaatga
     3481 ttgatttcaa accgcataaa attgcaatta ctctgtcaaa tccaactgca gcaccgccgt
     3541 gtggaggcgc accatatttg aatgcttcca gtataaatcc aaatttcatt tttgcttcct
     3601 catctgttaa gccaataata ctgaatactt ttttctgaat ctcaggtttg tgaatcctta
     3661 tactgccgct tgttatttca tgaccgttta gtaccaggtc ataacagttt gcgcggatgc
     3721 tctcgatttc atttatttct ttactgtcga gatattttat ataatcatcc ttaggcatgg
     3781 taaatacgtg atgctccgca taaaatcttt tgtcctcttc ctcatatctg aacagaggaa
     3841 aatcaattac ccatacaaat tcaaatcttg attcatctgt aagtgcaaat tcatttgcaa
     3901 gtctaagcct gagctgtcca agtgactgaa gcactttcat tctttcacca ctcagaataa
     3961 atattgtatc acctttctcc gcttcaaatt tctttttaat gttttctata atttcgtcat
     4021 tcagaaactt tacaattggt gactgcactg atccgtcttc attaaatttc agataaccaa
     4081 gacctccgaa tccaaacttt tttacaaact cagtaagtcc gtcaattatc tttcttgaaa
     4141 cttctttaga agatagtttt atgcctgctg ctattccacc gttatcaata gcatcagaga
     4201 ataccttaaa ttctgagact tttacaattt ctgaaatatt ttcgattttc atttcgcctt
     4261 caactctgag atcaggttta tctgatccat aactgttcat ggcttcatca tatgtcatcc
     4321 ttcgaaattt ttccggaaga tcaatccctt ttatttcttt ccacatttct ccaaacattc
     4381 cttccagtat ttcaaacaca tcatcctgat ctacaaagct catttcgaga tcgatctgag
     4441 taaactccgg ctgtctgtct gctctgagat cttcatctct gaaacattta cagatctgaa
     4501 catatttgtc aaatccggac accatcagta tctgtttata aatctgaggc gactgcggca
     4561 gagcataaaa ttttccgtta tgaactcgtg atggaacaag ataatctctt gcaccttcag
     4621 gtgttgattt cataagcatt ggagtttcca cttccagaaa tccatgcttc tcaaaatatt
     4681 tatgaatgat gtttgatgct ttacttctga ctacaaaatt atccgtaagc gaatttctgc
     4741 ggagatctaa ataacgatat ttaagccgaa gttcatctga tgctttaata tcatcttcca
     4801 ctacaaatgg aggaatatca gattcattta gaatttctaa atcagtaagc tcaatttcaa
     4861 tttcacctgt aggtatattt ttatttatac tttccctttt taaaactttt ccggtagcgg
     4921 aaattacaaa ttcctgacca agctttaaag cagtatcata aatttcttta ttttcaggaa
     4981 ttgccttaag ctgtgtaatg ccatatctat cgcgtaagtc aataaacagc aatcctccga
     5041 gatcacgctt ttttgagacc cacccgttta aagtgattat ttcattttca ttttttaaat
     5101 ttagctctcc gcagttatgt gtacgtttcc tgaaattcat ctgtttaatt gtaatttttt
     5161 gttatataaa aaagggctta aatttttata agccctttat aaattatttt gatttaaagt
     5221 taagtattaa ttctaattaa aattaagaag ttgaatatcc ggtaaaaaaa tttgtttagg
     5281 aattttcaat tcgtaattat taatttttaa ttgactaaag cgttcctctg agatcctgct
     5341 ctctttcaat agcttcaaac aatgctttga aatttccttt tccaaaactt ctcgcacctt
     5401 ttctctgaat gatttcgaaa aatactgtcg gtctgtcttc aacaggtttt gaaaatatct
     5461 gaagaagata tccgtcttca tccctgtcta caagaattcc cagcttttcc agatccgaaa
     5521 tatcttcgtc gatctttcca actctttcca caagctcttt ataatatgta gttggtattg
     5581 tcaaaaactc aagtcccctg ctcttaagtt catttacagt ttccagtata tttgcagttg
     5641 acatcgcgat atgctgcgcg ccggcagatt tataaaagtc aagatattct tcgatctgag
     5701 atttcttttt tcctgctgca ggttcattga taggaaattt tattttacct gttccgtttt
     5761 gcatcacctt gctcatcaaa gctgtgtaat ctgttgaaat atctttatca tcaaaagaga
     5821 taagctgatt gaatcccatt gtgtgtgcat aaaatttcac ccagtcgttc attgttccaa
     5881 gttcaacatt tcctacaata tggtcaactg ctttgagtcc tgtatctttt atacctgcta
     5941 ctgatctgtg ttcagcactt acaaaacccg gcatgaataa accgttataa ttttttcttt
     6001 caacaaaaga atggatcgta tcaccgtaag tctgtattgc agatctgata agtactccgt
     6061 tttcatcttt aaattctgtc ggctcgagaa cactttttgc cccccggctt gtagtttcga
     6121 aatatgattt ggttgcatca tccacttcaa tagcaatatc tctgactccg tcaccgtgtc
     6181 ttttgtgatg ttcggctatg aagttatcat cagtcagagg tgaagataaa acaaatttaa
     6241 ttttaccctg ttctaaaagg taagaagtct tttccctgtt acctgtttca agtcctgaat
     6301 atccggtcag cttaaaaccc attacatatt gataaaaaaa tgtagactgc tttgcattac
     6361 cgcaataaaa ttctaaatga tcaattcttt ttaaggggag aaaatctttg gcagtatctg
     6421 catcctgaac ttttacttcg gtttcgttta tagtttccat aactattttg gttaagaatt
     6481 aaaaaatatc tgaatgattt atctaaattt aattaattta tgtttaaaat ttaatgcata
     6541 tctcaaataa aaatcccggc taagagtgcc gggataaaaa ttactgctga cgaattttat
     6601 atttctaaat taattaagga gttatttttg aaataaaatt tgatacattg ttatctgaaa
     6661 tttccagatc actaatgtcc actaatccgt ctctgtttat atctgtatca ctaaaaccgg
     6721 ttaataaatt aaacgcatca ttttcaattt tatacatatc aaataaatca atatatccat
     6781 ccatgtttac atctccgcta aagatactat ataactgatt tattaatttc atattattcc
     6841 cgtatgcctg agactgtgaa gttgtgaaat cataattaat aatttttcct gctgatacag
     6901 aaactccgcc gttattactc catgtctcta tactgttttt accttttaaa actatgtaat
     6961 aatttcccga gcttagtttt gaggttgaga aatttccagt aaatgtcaaa gagtcaaata
     7021 tgactttacc ggaatctttg atctgaaacg ggtaaaaatt atttctaata taaactgata
     7081 ctgtatctga taaatttaat ctgttataag tagtattaaa attaccttca gaaattgctt
     7141 taatatttat ataagtcaac ggattatcag tgatcggaaa ttcatgaaat gaatacgctc
     7201 caatccccct gttatttcta agaaatggtc cgagatacac agcagaatca atatgtgtcc
     7261 agactgtcgg taagtaagta agtttggtag cactgctggg atttgtgagt ttcaatttaa
     7321 ttgtatctcc ggatttttgt cctgatgaaa tcaatccggt ctgtccattt aaataaaaat
     7381 aatttttcat actattacct aaagaatctg ccggccagta tgaaacggtt gaattattaa
     7441 aaagtaaagc tatttcattt ttctgagaat tagtataaaa ggcagtcctg atattcggac
     7501 cgatcttacc cgtagtatca acaggtgtat aaaacttttc acttatgtat ttgaaaacaa
     7561 ctgttccaat atttttatat ccattatact gataatgaca tcctccataa tagggtaatc
     7621 ctgctgttgc aataacttcc agatctggaa atgactcagg taattttctc tgtacttctc
     7681 tgagctggct tgcatactga gcagaacagc aaggtcttgt ctgaaataca tatgtaaaat
     7741 caaatccggg ataattttct ttccattgat tatataatgt ctgaaaattc tgctgataat
     7801 ttaaccagct tattgttccg ttcgattcac cctgaaacca gaatatggct tttactttat
     7861 ctgtaagtcc tgcttttttg gttctgtata attgtcttcc ataaagagta ttgaaatcgg
     7921 tatgattgga attgtttctt aaattcagtt ctatggtaga agcagctcca cctccgttta
     7981 taatacaggt cggaatgcca aaattttctt ttattaattt ctgaagttca agtccccata
     8041 ttccaacatt gaacggaccg ctaaatactc tgactgcacc atctgctttg gataatcccc
     8101 acaaagtatc tacaggatta tatggaaatc cattataatt tagtgtctgc attccaaaag
     8161 acctgcaaaa ctcattttca taggtcatta aagcatctgt aggcgtggaa tttgattgcc
     8221 cggcaatcat aaaaaaatct ccgcagacta cattatttgc aatgcttata agtgtacttg
     8281 aagaaccaga tcttaagtaa aatttgaaat tgaattctgt taattcggca tggatttttt
     8341 gcttaagatc aaaccatgat ttaccgttag cataaactaa ttttgcagat cttcttttcc
     8401 ataaagttga atttctgtaa acttccatgt atacagaatc atatcctgtt acatttattg
     8461 ttcctgaata tgttacaaaa gcagaatcct gcgtgtccct cgcataaagt tgatatgtcc
     8521 ttggaaaatc agaaaaagtg tatgtagatg aattgattcc ggaaatggaa ttcaaggata
     8581 actgatctgc aaatgaattt tgaaagctta gaaatgagaa tgaaattaaa aaatataaaa
     8641 ccccaaatgt aaaatactta atttttttca tactatttta taaaccaccg ctattttcta
     8701 tttcggatat agaaaaggga aatttaatct aaggaatgtg cattaattag aagttaagat
     8761 aaatatccat agaaatgata ttatatatat cctttctttt atttaaaaaa taatttttat
     8821 gatgcataag atagaaattt atattttaaa taaaaatata tttttaatga atgatttact
     8881 ttaagtaatt catgcatctg taatatatta tttttataaa ttaggatttt taaacaaatc
     8941 agaaattgaa ttctgctaac cgccttaaac taatagcttc aattgcattt attcgatttt
     9001 cttctttaat taaatttaaa atatctttaa ttgattatct ggttttcata acgataattg
     9061 ttatatcatc atgaacttca gcataacctc tgaatttatt tatttcatta aggtatattt
     9121 ccttaatttc ttcagcaggt ctgaaaaaat tttcacttaa aattttcttt acgttttcaa
     9181 gtccaaactc ctctttttga ctgttcatag attcagttaa tccgtctgaa taaaaacaaa
     9241 atacatctcc cgggtttgcg ttaatattaa tttcctctaa agaattgtca aacatctcac
     9301 catcagttaa tccaactgcc attccttttt gttgaatttc tctgcactct cctgcaaaac
     9361 tatcatacat tagtaatggc gtatgacctg ccctgcagat cttaagattt cttttattta
     9421 cttccactaa tattgaactg aaataaagtc ccttttttat atgcctgaat acgtttttgt
     9481 ttagaccttt taagatacag catggattgt cataaatatc tgttaactgg cgaataagaa
     9541 gtctgacctg aatcatatat aaagctgctg acattccttt cccggaaata tcacccgttg
     9601 aaatgaagta aatctcatca ctttttttta taaaatcaat aaaatctccg ccaacgatct
     9661 tagctgtttc ataataatat gatatatcaa aataattatc ttccggagga ttactgggta
     9721 tgagactggt ctgaacatcc ctggctattt tgagttcgtc acctgatgtc agcttttcag
     9781 caatttcata aataagtata aaatttacaa ttatgaatgt cagaatagca agtttccagt
     9841 ttccgtttac ccaggcaaat aaaaatgcta ttacagctaa agaataaaaa attcttatta
     9901 caggtgatag ttttgtaagg aaagctattg tgaaattttt taaaagctta aacaagccag
     9961 tctttttaga agca
//
LOCUS       DAORVQ010000119         9878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3658_length_9878_cov_5.296447, whole genome shotgun sequence.
ACCESSION   DAORVQ010000119 DAORVQ010000000
VERSION     DAORVQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3658_length_9878_cov_5.296447"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLX80_09880"
     CDS             complement(<1..1033)
                     /locus_tag="PLX80_09880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY21139.1"
                     /translation="MTLTNLPADKKISYMPQLDTLRAFSLFLVLLGHWINTDSWIHKF
                     PIGIAGVTIFFVLSGFLITGILLRNKVSLDSGNHKLLYYLKNFYIRRTIRIFPVYYFT
                     LIVLFILNFENIREIILWFVFYISNYLMYIKMDWTGALSHFWTLAVEEQFYLFWPLII
                     LFTPKKHLFKVIILFILAGIFFRGYNYLINDKTEYHTMFSYMLTLSCIDGFALGAILA
                     YIRNYIDDMFDLLKGKYIFILIGTFLLMIILMFFDINVVDVSMFKFCIAVIALFMVSK
                     LSIGFKGKLKYVFENKFLMYLGKISYGMYIFHTFVPYIYLSLGLPYFDSMLIMFLIYL
                     LILIVISSLS"
     gene            complement(1039..2115)
                     /locus_tag="PLX80_09885"
     CDS             complement(1039..2115)
                     /locus_tag="PLX80_09885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY21140.1"
                     /translation="MINVTKTFLPPAEEYNEYLKKIWETHWVTNQGPLCEELELKLKK
                     YLGVKNLLLVSNGTIALQLAIKALDLKGEIITTPYSYVATVNSIIWENCTPVFCDIED
                     KTFCMDAEKIEKLITDKTSAIMPTHVYGFPCDVNRIKDIADKNSLKIIYDGAQSFGVK
                     VNDTSVYNFGDISTISFHATKLFHTIEGGAVITDDDALAEKLFLYRAFGHMDDDYYSV
                     GINARTSEFNAAMGLCNLHRVMGFIEERKRLNLLYKKFLKDLPLKFPEIPTDIFYNYS
                     YFPVFFQTEEIMLEVKDLLFKNGINTRRYFYPSLNTLAFTYGESCPVSESAANNVLCL
                     PIYNEMDSGLPEKISSLISQALNL"
     gene            complement(2112..3404)
                     /locus_tag="PLX80_09890"
     CDS             complement(2112..3404)
                     /locus_tag="PLX80_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21141.1"
                     /translation="MSNIAISTDKLSKAYLIGLKNETHDTLTGAISSWLKYPLKNYKT
                     LSRLNTFKRKEEGDDYFWALKDVSFEVKHGEVLGVIGPNGAGKSTLLKILCRITEPTS
                     GSARVYGRVASLLEVGTGFNVELTGRENVYLSGTILGMTKKEIDKKFDEIVDFSGVEK
                     FLDTQAKKYSNGMRIRLAFAISASIETEIIMIDEVLNIGDTDFQRKSIAKMESLAKEG
                     RTVLFVSHNLSAIKAICTRALQLNHGKLIREGEVSKVISVYLSKYVKEYATQNFDLET
                     APSSEYAKLIKAELKFDETKNDHLIAGDPFEFEFVFFNMMKEEHILDVVFQLKDELDN
                     IVFIGSSLDNNYEKISEGYFKWTCKVPPDIMNQGSYTLHKFYLLKNKTDVIFEQREVL
                     SFEIMYAFDLRIGDVGRKEGIIKPKLNWDLLIGENANK"
     gene            complement(3436..4281)
                     /locus_tag="PLX80_09895"
     CDS             complement(3436..4281)
                     /locus_tag="PLX80_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21142.1"
                     /translation="MPSEVTPYFEIKPRKSWRLIDFREFVKYKDLLYFFVYRDITVLY
                     KQTVLGLLWAVLNPVVSMVIFSVIFGGLAKIDSDGIPYPIFSYAAVLPWTYFSQSMGG
                     ATSSLVGNANLLTKVYFPRIFIPLTPVLSKLMDFAIAFVILIAMMLFYQISPTLNVLF
                     LPILMILMILTSLGLGLWLSSLAIQYRDVRFAVGFLTSILMYAAPVVFPASLVAEKFG
                     DTAYLLYGLYPMVGVIEGFRASLLGFNPMPWDLIGMGSISAIIIFISGAFHFKKTEKI
                     FADVA"
     gene            complement(4333..9174)
                     /locus_tag="PLX80_09900"
     CDS             complement(4333..9174)
                     /locus_tag="PLX80_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQY21143.1"
                     /translation="MNKTLILLSLLFLSVTNSYSQNFNWITPNKTYLKLYVIEDGIFR
                     INKSDFTNNGINPSGIDPRTVKVLNKGNQIPVFFQGENDGTFDDADYLDFYGQRNYGG
                     PTKHYNGYSNSVIYVTDEKYDSYSDTNVYWIDWGGGLGNRMEISNYVSSLNYPEDFSM
                     QPVHFEEDHIYYLGETTNSNTDFRYFSNEKVAGESWFWKIMTTNETQTSSFTPINVNG
                     SQTCEIKLLLFPVSYNIQASNEHVVEVKVNNTLIGTMYADSLRRMDTLLYFPASLLNS
                     GANNDISITYTPVTGNFFQPRIHLDFFELKYPRAFSFTNNFANIKLNGSDSTSKKINV
                     TGALSSNPVSIYDISNGIKIENQTINQNTLTFTGKSNSEFELANNNITKKPFRIENRV
                     VSDLVSSSNGADYLIIYHKLFESAAQQLKGHRENFNNFRVKNAEVKDIYDIFNYGIEN
                     PVALRNFVSYAYNNWSGAKVQFVNLFGRASLDPKKNSSASVYYQNFIPSYGNPPTDGY
                     FVNFNFGTFTYYHQISIGRLPVYTSVEAQDMVNKIITYDLQPPQKWWKNYLSITGGGN
                     RQEQLNFQAKSENFIGNYFAPAPASMNIARIYRNDSSGYVTYNYKDSIKKEFDRGQMI
                     VNFIGHAAAQDWEIGLEDPSTMENGEKQPLILSFTCFTGQNSETNKRSFGEKFILLPN
                     KCAIGFVGTTGWSFSGIGDAYNDRLIKRFTKDSVRYVGDLMSYASQQIGQDSNSFSAR
                     NTVNCYNLLGDPATQLLMSPTPEFDIKQNDYILSNSFPALGEDVKLNIFPKNLGTFAD
                     SVRIRFNIKKDGALSQSRDTVFKFFGFVDTISHFFKIDTIGNYNMTVIMDPNKFYQQK
                     FYDNDSITFPLTLRNISFTQIKPLNNAVVTSSEFKFTGLNPNVDYRTSNIKLIFQIDT
                     IESFNSPILQTYNNSNITGVSSSFNVSLPVTNLNTLYYLRTNAIIGNDSSGWSENTNI
                     VYNPNVSVNQDGVNDSAYTVYKSDPRQFGESSVSNLVYKDGGYKLDEFKGNLFIRSWG
                     SNGFESSFFTINDFNYYSDGGFNRGLNLAKVKKLTGVPTEIKSFVFTSPASSDSVLNF
                     LNTFDSTDYLLGYNASYVADSDSLRPDALAKFREFGSIGIDSIRIGFFDTWAFFGYLG
                     ADTNNTCEHFHLFFSNISWEPQECQVSPFFIEPEGYVVQEFGTADKWKDYSWEQSVLP
                     TNSIDLDIYGINSDNNIVSLATGITNNTSVTLDTIDALQYPKLRLVNNLRIDTVSGLE
                     SPVFSSAKIKYVPPAEITPDNYSFTGSDTVVQEGDSVNFSVNYYSIGFRDIQNYINYW
                     YVSVQGKNIYLKIDTINTPLRIDSMGTSSVKFSTAGLREFESDSDTLDVYFETEVLGD
                     YNEMFTYNNKAITQFIVQGDSINPIINVTYDGVEIINGDNIQSTPNIVLEFLDDSRMV
                     INDTSNVRVFLDNKYVPYFINGIQNPIIQLIFPDNNTLQATLIYNPTLSEGNHKFRFM
                     ATDITGNLADSIVNTVVVENDLSISGMANYPNPMGTETNFMFRITGEFPPSSCKVKIF
                     TVAGRLIKEISSSVIVGYNSIYWDGKDNDGDYIANGTYLYKFIVQGNSQVETITQKLV
                     ILR"
     gene            complement(9282..>9878)
                     /locus_tag="PLX80_09905"
     CDS             complement(9282..>9878)
                     /locus_tag="PLX80_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HQY21144.1"
                     /translation="KIYSDISLKKMYESFDLIHPDGVGVFYASKYLYGTDGMNERFAG
                     SDFYISLISSSISEGRSIFFFGHTLKELDKIKTNYPALNIAGIQEGYNFDTDEVIEKI
                     NRSNPDIIIIGLSCPVQEKWMYENKDKIKFKVMLAVGDGIKVFSGDKIRGPKILQKAG
                     LEWMVRVFTDPLNNFNRYVKGIPIFIQRIKRYKQSLIK"
BASE COUNT         3206 a         1760 c         1613 g         3299 t
ORIGIN      
        1 aggataatga agaaatcacg atcagaatga gaagatatat taaaaacatt attagcattg
       61 aatcaaaata gggtaatccg agacttaaat aaatgtacgg aacgaatgta tgaaaaatat
      121 acataccata gctgatcttt ccgagataca tcaggaattt attctcaaaa acatatttca
      181 gtttgccttt gaatccaatg cttagtttag aaaccataaa taatgctatc acagcaatgc
      241 aaaatttaaa cattgaaaca tccacaacat tgatatcaaa aaacataaga atgatcatta
      301 aaaggaatgt accaattaat ataaaaatgt atttaccttt taagaggtca aacatatcgt
      361 ctatataatt tctgatgtag gcaagaattg cgcccagagc aaatccatca atacaagaca
      421 aggtcaacat ataggaaaac atcgtgtgat attctgtctt gtcattgatg agataattat
      481 atcctctgaa aaatattccg gctagtatga aaagaataat aactttgaat aaatgttttt
      541 tgggagtaaa caaaattatc aatggccaga agagataaaa ttgttcttcc acagcaagcg
      601 tccagaagtg tgacaaagca ccggtccagt ccattttgat atacatcaga taatttgaaa
      661 tataaaatac gaaccaaaga ataatctctc ttatattttc gaaatttaaa ataaaaagaa
      721 caattaatgt gaagtaatat acgggaaaaa ttcttatggt ccttcttata taaaaattct
      781 ttaagtagta cagcaattta tgattacctg aatctaaaga aaccttattt ctaagcagaa
      841 tacctgttat caaaaatccg ctgagtacaa agaaaattgt tacacctgca attccaattg
      901 ggaatttatg gatccatgaa tctgtattta tccagtgtcc gagcaaaaca agaaataatg
      961 agaaagctct aagagtatcg agctgaggca tgtaagaaat ctttttgtca gccggtaaat
     1021 ttgtaagtgt caagataatt acaaatttaa agcctgtgag atcagggaag aaattttttc
     1081 aggaagtccg gaatccatct cattataaat agggaggcat aaaacattat tggcagcaga
     1141 ctcagataca ggacagcttt caccgtatgt aaatgcaaga gtattaagtg aaggataaaa
     1201 atatcttctt gtgttaattc cgtttttaaa taataaatct tttacttcca gcattatttc
     1261 ttcagtttga aaaaataccg gaaaatatga ataattgtaa aatatatctg taggtatttc
     1321 agggaatttc agaggaagat ctttaaggaa ttttttgtac agcaaattca gtcttttcct
     1381 ttcttcaatg aatcccatta ctctgtgaag gttacacaaa cccatggcag cattgaattc
     1441 cgatgtcctt gcatttatcc cgaccgaata ataatcatca tccatatgtc cgaatgcccg
     1501 gtagaggaat aatttttcgg ctagagcatc atcatctgtg atgacagcgc cgccttctat
     1561 tgtatgaaat aattttgtag cgtgaaaact tattgtcgaa atatcaccga agttataaac
     1621 agaagtatca tttactttta ctccaaacga ctgagcgccg tcatagatta ttttcaaact
     1681 atttttatct gcaatatctt ttatgcgatt cacatcacac ggaaacccgt aaacgtgggt
     1741 tggcattatt gctgaggttt tatcggttat taacttttca attttttcag catccatgca
     1801 gaaagtttta tcttctatat cacaaaatac aggtgtacag ttttcccaga taatagaatt
     1861 tacagttgca acatatgagt aaggagttgt aatgatctcc cctttaagat cgagagcttt
     1921 gatggctaac tgaagtgcga ttgtaccgtt tgaaacgagc aggagatttt ttactccgag
     1981 atattttttt agttttaatt caagctcttc gcaaagaggt ccctgattgg taacccagtg
     2041 tgtttcccag atctttttga gatattcatt gtattcttca gccggtggta aaaatgtttt
     2101 tgtaacatta atcatttatt tgcattctca ccaattaaaa gatcccagtt taattttggt
     2161 tttattatgc cttccttcct gcctacatct ccgatacgca gatcaaatgc atacatgatc
     2221 tcaaatgaaa gtacttccct ttgttcgaag atcacatcag ttttattctt gagtaaataa
     2281 aatttatgta aagtatatga accctgattc atgatatccg gcggtacttt gcatgtccat
     2341 ttaaaatatc cttcactgat cttttcataa ttattgtcaa gactggatcc gataaataca
     2401 atattatcta attcatcctt aagctgaaat acaacgtcaa gaatatgttc ttctttcatc
     2461 atattgaaaa atacaaattc aaattcaaaa ggatctccgg caataaggtg atcatttttt
     2521 gtttcatcaa atttcagttc tgctttaatg agcttagcat attcactgct gggagctgtt
     2581 tcgaggtcaa aattttgtgt ggcatattct ttaacgtatt tgctgagata tactgaaatg
     2641 acttttgaaa cttcaccttc acggatcagt tttccatgat tgagctgcaa agctctggta
     2701 catattgctt tgattgcaga cagattgtga cttacaaata aaacagtcct tccttctttt
     2761 gcaagacttt ccatttttgc aatactcttt ctctgaaaat ctgtgtcacc aatgtttaaa
     2821 acttcatcga tcattatgat ctctgtttcg atactggcgg aaattgcaaa ggcaagtctg
     2881 atacgcattc cattagaata cttttttgcc tgagtatcga gaaatttttc tacacctgaa
     2941 aaatcaacaa tctcatcaaa ttttttatcg atctcctttt ttgtcatacc gaggattgta
     3001 ccgctaagat acacattctc cctgcctgta agttcaacat taaatccggt accgacttcg
     3061 agaagacttg caactcttcc ataaactctt gctgaacctg aagtaggctc agtgatccgg
     3121 cagagtattt tcaataatgt acttttgccg gctccgtttg gtccaattac accaagcact
     3181 tcaccgtgct ttacttcaaa agatacatct ttaagcgccc agaaataatc atcaccttct
     3241 tcttttctct tgaaagtatt tagtctgctg agagttttat aattctttaa tggatatttc
     3301 agccaggaag agattgctcc agttaaggta tcgtgagttt cgttttttaa acctatcaga
     3361 tatgctttgc tcaacttgtc tgtagaaatt gctatatttg acatttaata ataattttca
     3421 ataatttgtt ttggatcagg caacatctgc aaatatcttt tctgtttttt tgaaatggaa
     3481 agctccgctg ataaagatta taatggcact aattgaaccc attccaatca agtcccacgg
     3541 cattggatta aatccaagta atgaagctct gaatccttct atgactccaa ccattgggta
     3601 taaaccatat aaaagatatg cagtatctcc aaatttttca gcgactaaag aagccggaaa
     3661 tacaaccggt gctgcataca ttaaaataga ggtcaggaat cctactgcaa atctgacatc
     3721 tctgtactgg attgcaagtg aagaaagcca caatcccaga ccaagtgatg taagtatcat
     3781 tagtatcata aggatgggga gaaataaaac attcaaagtc gggctgatct ggtaaaacag
     3841 catcattgct atcagaataa cgaatgcaat tgcaaaatcc ataagtttgg aaagcaccgg
     3901 agtaagcggt atgaatattc tcggaaaata tacttttgtt aaaagatttg cattcccaac
     3961 aagactgctt gtagcgcctc ccatagactg tgaaaagtaa gtccacggaa gtactgcagc
     4021 atatgaaaat ataggataag gaattccgtc actgtctatc ttggcaagac ctccaaatat
     4081 taccgaaaaa atcaccattg aaactacagg atttaaaaca gcccacaata atccgagtac
     4141 tgtctgtttg taaagcactg taatatctct gtaaacaaaa aaataaagaa gatctttata
     4201 ttttacaaat tctctgaaat caatcaacct ccaggatttc ctgggtttaa tttcaaaata
     4261 aggagttact tctgaaggca tttaagattt aaatttttct tatacaatta atttctgttc
     4321 taatctaata tattatctga gtatcactaa cttttgcgta atagtttcca cctgagaatt
     4381 tccctgaacg ataaatttat aaaggtaagt tccgtttgca atataatctc cgtcattatc
     4441 tttcccatcc cagtatattg aattatatcc tactataaca cttgaactga tctctttaat
     4501 aagtctgcct gcgactgtaa aaatctttac cttgcaggac gacggtggaa actctcctgt
     4561 tatcctgaac ataaaattag tttccgtacc cattggattt ggatagtttg ccattcccga
     4621 tatactgaga tcattttcta caacaacagt atttactatt gagtcggcta agtttcctgt
     4681 tatatcagta gccatgaatc tgaatttatg attaccttca gatagagtag ggttataaat
     4741 taatgtagcc tgtaaagtat tattgtcagg aaaaataagc tgtattatgg gattctgaat
     4801 tccgtttata aagtaaggca catatttatt atcaagaaaa actctgacat ttgaagtatc
     4861 atttataacc atccggctgt cgtcaagaaa ttcaagaact atattcggag ttgactgaat
     4921 gttatctcca tttattatct caacaccatc ataagtaaca ttgattatcg gatttataga
     4981 atctccctga acaataaact gagtaatagc cttattgtta taagtaaaca tttcattata
     5041 atctccaagt acttctgttt caaaatacac atcaagtgta tcactgtcag attcaaattc
     5101 cctgagtccc gctgttgaga attttactga tgaagtgccc atgctgtcaa ttctcagcgg
     5161 agtattgatt gtatcgatct taagataaat attttttccc tgcactgaga cataccagta
     5221 atttatataa ttctgaatat ccctgaatcc gatactgtaa taatttacag agaagtttac
     5281 actgtctcct tcctgcacga ctgtatcact gcctgtaaaa gaataattgt ccggagtgat
     5341 ctcagcaggc ggtacatatt taatttttgc agaactgaaa acaggggact caagaccgga
     5401 gactgtatca attcttaaat tatttaccag cctcagtttt ggatattgca gagcatctat
     5461 tgtatcgagt gtgacagatg tattgttagt tatgcctgtt gcaagagaca caatattatt
     5521 gtcagagtta atgccgtata tatcaaggtc tatagagttt gtcggaagga ctgactgctc
     5581 ccatgaataa tctttccatt tatcagcagt tccgaattcc tgcactacat aaccttcagg
     5641 ttcgataaag aaaggactaa cctgacattc ctgcggctcc cagcttatgt ttgaaaaaaa
     5701 caaatggaaa tgttcacagg tattatttgt atcagctccg agatatccga aaaatgccca
     5761 agtgtcaaag aatccgatcc ttatagaatc aataccaata cttccgaatt ctctgaattt
     5821 tgcgagcgcg tctggcctta agctgtctga atcagcgaca taagaagcgt tgtagccgag
     5881 cagataatct gtggaatcaa atgtatttaa aaaattcaaa acagaatcgg aagaagcagg
     5941 ggaagtgaac acgaaactct tgatctcagt tgggacacct gtgagctttt taacttttgc
     6001 aagattcaga cctctgttga aacctccgtc actgtaataa ttgaaatcat tgattgtaaa
     6061 aaatgaagat tcaaaaccat tgcttcccca agaccggatg aacagatttc ctttgaattc
     6121 atcaagctta taaccgccgt ctttatagac taaatttgaa actgaagatt ctccaaattg
     6181 tcttggatca gatttgtaaa ctgtatacgc tgaatcattt actccgtcct ggtttactga
     6241 gacattagga ttgtaaacta tattcgtatt ctcagaccat ccggaactgt cgttaccgat
     6301 gattgcattt gtccggagat aatataatgt gtttaaattt gtgacaggca gagagacatt
     6361 aaatgaagaa ctaacacctg ttatattaga gttattataa gtctgaagta tgggagaatt
     6421 aaacgattca atagtatcta tctggaaaat taattttata ttacttgttc tgtagtcaac
     6481 gttaggattt agcccggtga atttaaattc ggatgaagta acaactgcat tatttaaagg
     6541 ctttatctga gtaaaagaaa tatttcttaa agtaagcgga aatgttatgg aatcattgtc
     6601 gtaaaatttt tgctgataaa atttatttgg atccatgatc acagtcatgt tataatttcc
     6661 aatggtatca attttgaaaa aatgacttat cgtatctaca aatccaaaaa atttaaacac
     6721 agtatctctt gattgtgaaa gtgctccgtc ttttttgata ttaaatctta tccttaccga
     6781 gtccgcaaat gtccccagat tttttggaaa aatatttaat tttacatcct cgcctaatgc
     6841 aggaaaacta tttgataaaa tataatcatt ttgttttata tcaaactccg gggtaggact
     6901 cattaataat tgagtagcag gatcaccaag aaggttataa caattgacag tatttctggc
     6961 agagaagctg tttgaatcct gaccgatctg ctgggaagca taagacatca gatcacctac
     7021 ataacggaca ctgtcctttg taaatctttt aataagtctg tcgttatatg catcacctat
     7081 tccggaaaag ctccagccgg tagtaccgac aaaaccaatt gcacatttat taggcagaag
     7141 aataaatttc tctccgaatg aacgcttatt tgtttctgaa ttttgacctg taaaacatgt
     7201 aaagctcaat atcaacggct gcttctcgcc attttccata gtggatggat cttcaagtcc
     7261 gatctcccaa tcctgtgctg cagcgtgacc aataaagttt acgatcatct gtccgcggtc
     7321 aaattctttt ttaattgaat ctttatagtt gtaagttaca taaccggatg aatcatttct
     7381 gtagattcgg gctatgttca ttgaagcagg cgcaggtgca aaataattac cgataaaatt
     7441 ttcagacttt gcctggaaat tcagctgctc ttgtctgttt cctccacctg taattgaaag
     7501 ataatttttc caccacttct gaggcggctg cagatcatag gtaattattt tatttaccat
     7561 atcctgtgcc tcaacagatg tataaacagg aagtcttcca attgagatct gatggtaata
     7621 tgtaaaagta ccaaaattga aatttacaaa ataaccgtct gtaggaggat tgccatatga
     7681 tggaatgaaa ttctgataat aaacagacgc cgaactgttc ttcttcggat cgagagatgc
     7741 ccttccaaaa agatttacaa actgtacttt cgcaccagac cagttgttat atgcataact
     7801 aacaaagttt cttaaagcaa cagggttttc aattccataa ttaaaaatat catatatatc
     7861 ttttacttca gcatttttta ctctgaaatt attaaaattt tctctgtgac cttttaattg
     7921 ttgagctgcg gattcaaata atttgtgata tataataaga tagtctgcac cgtttgaaga
     7981 tgagacaaga tcgctgacta ctctgttttc aatacggaaa ggttttttgg taatattgtt
     8041 atttgctaat tcaaattctg aattactttt gcctgtgaat gtaagtgtgt tctgatttat
     8101 tgtctggttc tcaattttta ttccgttgct gatgtcataa atactcacag gatttgaaga
     8161 caatgctcct gttacattaa ttttcttaga agtagaatca cttccgttga gttttatgtt
     8221 tgcaaaatta tttgtgaaac taaatgctct tggatatttt agctcaaaaa aatctaagtg
     8281 tattcttggc tgaaagaaat taccggtcac aggagtatat gtaatcgata tgtcattatt
     8341 tgctccgctg ttaagcagag atgcaggaaa atataaaagc gtatccattc ttcttaaact
     8401 atcagcatac atagttccga taagtgtatt gttaactttt acttctacca catgttcatt
     8461 acttgcctgt atattataag ataccggaaa aagtaaaagt tttatttcac aggtttgaga
     8521 cccgtttaca tttattggag taaaagagct tgtctgagtt tcattggttg tcatgatctt
     8581 ccaaaaccag ctttcgcctg ctactttttc attgctgaaa tatctgaaat ctgtatttga
     8641 gtttgtagtc tcgccgagat aataaatatg atcttcttca aaatgaacag gctgcataga
     8701 aaaatcttca ggataattta aagatgaaac atagttcgaa atttccattc tgttccctaa
     8761 accaccaccc cagtctatcc agtaaacatt tgtatctgaa tagctgtcat atttttcatc
     8821 agtaacatag attactgaat ttgaataacc gttataatgc tttgtcggtc cgccataatt
     8881 tctttgaccg taaaaatcca aataatcagc atcatcaaat gtgccgtcat tttcaccttg
     8941 aaagaataca gggatctgat ttcctttgtt aaggactttg accgttctcg gatctatacc
     9001 ggatggattt atgccgttat tagtaaagtc ggatttatta attctgaaaa ttccgtcttc
     9061 tatgacataa agcttaagat aagtcttatt aggagtaatc cagttgaaat tctgagagta
     9121 tgaatttgta accgataaaa ataataatga cagtagaatt aatgtcttat tcataaattt
     9181 aagaggatat ttaattaaaa attagtatcc tgttttcata cactcctgat agtttttttt
     9241 gaataaattg actgtaaata tacaatattt catttataat ttcacttaat taatgattgt
     9301 ttatatcttt taatcctttg tataaaaatg gggattcctt tcacatatct gttgaaatta
     9361 ttcagtggat ccgtaaagac tctcaccatc cattctaacc ctgctttttg taaaatctta
     9421 ggtccgcgga ttttatcgcc tgaaaatact tttattccgt ctcctactgc aagcattact
     9481 ttaaatttta ttttgtcttt attttcatac atccattttt cctgaaccgg acagctaagt
     9541 ccgattatta ttatgtcagg attggatcta ttaatttttt caattacctc atctgtatca
     9601 aaattataac cttcctgaat tcctgctata ttcaaagcag gatagttagt ctttatttta
     9661 tctaattcct ttaatgtatg accaaaaaaa aagattgacc tgccttctga aattgaactg
     9721 gatataagac taatataaaa atctgatccg gcaaatctct cattcattcc atcagttccg
     9781 taaagatatt ttgatgcgta aaaaactcca actccatcag gatgaatcag atcaaaggat
     9841 tcatacattt tttttaatga aatgtcagag taaatttt
//
LOCUS       DAORVQ010000120         9838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3683_length_9838_cov_6.065011, whole genome shotgun sequence.
ACCESSION   DAORVQ010000120 DAORVQ010000000
VERSION     DAORVQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3683_length_9838_cov_6.065011"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLX80_09910"
     CDS             complement(<1..385)
                     /locus_tag="PLX80_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQY21145.1"
                     /translation="MYLDKIIQQRLDEKLEALAELRPVQTSLLKKLKHQIALEMTYNS
                     NAIEGNTLDLKETFLVISEGITIKGKSMKEHLEAKDHYEAIEYLNELVSEETKNSFTE
                     VTLRSLHQLIMRETYSEEAGKYRTGN"
     gene            complement(668..2197)
                     /locus_tag="PLX80_09915"
     CDS             complement(668..2197)
                     /locus_tag="PLX80_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HQY21146.1"
                     /translation="MKSGKILTILLVVIFLSMTGDLFSQAYPGYTLYSSSSTRTNLMD
                     LNNNVVKFWTHTRSGGYSVYLLRNGDLMRPALSTGSTINGGAVAGVVQKYSWDGPLIW
                     EYTYSSNTYRTHHDICPMPNGNVLLIAWEVKTATDAVAAGLNHSAVIWPDHLIEVQPS
                     GTNGGTIVWKWHFWDHLIQDFDPTKANYGVVGDHPELLDINVGSAGSGDWMHINGISY
                     NAALDQILISSHELDEVYVIDHSTTTTEAAGHTGGRYGKGGDILYRWGRPSNYRAPGS
                     QIFNVVHNSVWVPDSLPGGGNIMAFNNREGTNSSMVVEFQAPTDSAGFYSYTSGSAFG
                     PADTEWSYSASGFYSNHLGGNQRLANGNTMISESTSGNLFEVTSAGNIVWSFSPGGQI
                     VRVLRYGFDYPGLVAVSNITTSIPENYDLSQNYPNPFNPVTTIKFELPKQEKVTMKVY
                     DILGNEVRTLIDNESRNPGQHSVVWDGKFNSGLNAATGVYFYKLVSENFTKTMKMILA
                     K"
     gene            complement(2258..3835)
                     /locus_tag="PLX80_09920"
     CDS             complement(2258..3835)
                     /locus_tag="PLX80_09920"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQY21147.1"
                     /translation="MKKNLLVFSFLMLFAAGFIIPRSAFSQGAGDSLFTGIQVHNVNI
                     IFSQPNYWDSLTIYYNQGNEQFLAATVIANGTIINNVGVRLKGNSSYTHPNNKKSFRL
                     SFNEFVSGQKWNGLKGVHLNNFWNDPSFLREKIHLDYCQSVGIVAPRANYVRLSINDT
                     LFALYSLVEHLNGDFLETHFGDGDGDQFKAVDAFGATSNLVSDFRWYGADTSNYTNRY
                     EIKSDSKMGWDRLVSFIDSINHTTDAYNTFSSKVNLNSYYKAMAVDILMGNLDSYVYN
                     GRNFYIYSLAPDYKMNWIVWDASLSMGALPGGLSNIETLPVTYVSSDTGRPLFGQIIN
                     NSQLRNEYLMTFCEIFTDNFSSSKLFPKIDSITTIIRPYVMEDPRKMFSNAQFETNII
                     SDLVISGFRIPGVKSYINARRSSVASQLNTLGINCEVGIYNGNETVNDFNLYQNFPNP
                     FNPNTIISYQISVNNDVSLKVYDVLGNEVATLVNEKQTAGNYSVEFKGADLSSGLYFY
                     ILKTPNQSVTKKMLLVK"
     gene            complement(3909..5105)
                     /locus_tag="PLX80_09925"
     CDS             complement(3909..5105)
                     /locus_tag="PLX80_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21148.1"
                     /translation="MKRIFFTTLFILTSLSVVQSQLVQSNFTGDIVPQYMGSGTSTRL
                     PYVYRATVSGLLPNSYYRYYLNACIYTDFGGTNSGAGNPLFIHGNDFRYSTATNLSNP
                     GGYDSILTNGSGSYTGWFGFVNTGNARFTAGNHIYPTITLDSAGSGSTLYRFALNDSI
                     KVLAFSTSSDANSGTGLYGISLATPKNIITIYDNVSNSGRPLSMVFAESDGIDTTVLT
                     NLVKYYKDSVDSRDGRWGSIIPNELPGGVKRVNVLSLSDASVIKFRTDDDGIWPSGTN
                     TVNPAGGADNPLRLDEQDVPLLINTGNIIAENFRLDQNYPNPFNPDTKISFSIPLNGY
                     VKLKVYDVLGKEISVLVDGNINAGNYQSTFNGKDLQSGVYFYRMEFSGDKGITFSDIK
                     KLILVK"
     gene            complement(5137..7131)
                     /locus_tag="PLX80_09930"
     CDS             complement(5137..7131)
                     /locus_tag="PLX80_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21149.1"
                     /translation="MKLIKTIALFFFTITFTFPVNSQWIQNSTGINGGDVYCISRSGN
                     DIFAGTNLYGIYKSNNNGTSWQQTELSDKSVYSIYISGNNIFAGTNLYGLYYSNDNGN
                     TWQQSSMSSQVVSEITEINGTIFAATSEGVYKSVNQGVNWIQSGLNTFEIYSIAASGN
                     TLYAGVGIAKGVYISTDNGAAWTQTSLNNKDVLAISVNGNNVCAGTINSGVYYSANSG
                     ATWSQSSFNNSVVYSFSQTGNTIFSGTSSGVYSTANNGADWASAGISNEIVQSVNTNG
                     SQLLAGSKKGIYSSQNSGANWTKSELHNLSVFSVINNGGVFFAGSGENGVFRSADAGN
                     TWAQTNLNTNSIPSIVANGNRILAGADNGIQISTDNGITWSGTSLTNEVVYSLAINGS
                     TIFAGGGYGFHISTDGGYNWSGTSFHEVCLALVQSGNTLYAGTYNGVFYSTDNAVTWT
                     QTSLNNESVNALLVNGTNITAGVSFNGVYISNNSGTTWTQTSLNDKDVYSLAQSSGYL
                     FAGTYGFGVYMSADNGQNWIPRNQNLGEQYVTVLMVSANDLYAGTGANALWKRNISEI
                     IGINQISSNVPADFNLNQNYPNPFNPVTKIKYDILKSGIVKMSVYDLSGKVVRVLFNE
                     HHTPGTYETSFEGSGFSSGVYFYKLESGSFSEVRKMTFIK"
     gene            7769..8830
                     /locus_tag="PLX80_09935"
     CDS             7769..8830
                     /locus_tag="PLX80_09935"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY21150.1"
                     /translation="MHNSFSFSQTDLNQNKDQLISKTNELKEKFKFNAVIAGLWKGEQ
                     EIFTIALGESMTNYPATTDMHFRIGGVSEMFYGTLVMILAEQEKINLEDKISKFLPDL
                     PNADKVTIQMLIKNTAGYKDYVYNNKFVDLILKYPFRYITREEIISYGTPDDLNFPPG
                     TEQKYSHTEFTILAEVIERATGKKMFELYEENIFKPLGLTNTGYSNNANLPSPVLHSF
                     SSDRKVYEDATFWSPSWAGESGPLYSNLFDLAKWGPVFGKGKLLNENSFNTLLSRFEN
                     TGKPDLYFASGFVVANGWYLQNPSMNGYSGGYGYFPEEEITLIVYSTETADTPSDKKA
                     FDIFKELVKMVTPDSQINF"
     gene            8945..9031
                     /locus_tag="PLX80_09940"
     tRNA            8945..9031
                     /locus_tag="PLX80_09940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8979..8981,aa:Ser,seq:tga)
     gene            9165..9776
                     /locus_tag="PLX80_09945"
     CDS             9165..9776
                     /locus_tag="PLX80_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21151.1"
                     /translation="MKKLFIYSLILFIPIIISSCSGVNKTIENAQRLQFKLGKVDGLD
                     IAGVNLSNVKSLNDVNILQGANLLAAFASGRLPTTFTVNLIAKNPDTYPGGDAESTSL
                     IKSLDWRLLIDNKNIINGAITQPITIPGVGKSATIPIPISIDLLELFANNSYENIINL
                     ALAIGGKSGNSSRLTLKIKPTIDTFLGGISYPGEIDVIDKEFR"
BASE COUNT         3130 a         1830 c         1629 g         3249 t
ORIGIN      
        1 catttcctgt tctgtatttc cctgcttctt cagaataagt ttctctcatt attaattgat
       61 gaagcgatct taaggttact tctgtaaatg aattttttgt ttcttcagat acaagctcat
      121 tcagatattc aattgcctcg taatgatcct tcgcctccag atgttccttc atagattttc
      181 cttttatagt aataccttcg cttatcacaa gaaaggtttc tttgagatca agagtatttc
      241 cttctatagc attggaatta taggtcattt caagtgcaat ctgatgcttt aattttttca
      301 gtaaagaagt ctgcaccgga cgaagttcag ccagtgcttc cagtttttca tctaatcgct
      361 gctgtattat tttatccaga tacattttct taatatggtt taactgaata atatagctat
      421 accatattaa gaattatatg gtaatttcag atacatctat aaatttaaat cagaatttcg
      481 aaaacaaatg cctctaagac tcgaaagctc aaagttttaa aacaattaaa attgatgcgt
      541 ttgaaccttc gggtctttga agtatagacc aaagttcatg cggataataa aaaaagagct
      601 ccccaaaata tcaggaaagc tcttttaaag ataaataaaa ctaaactctt atttttaaca
      661 ccggaaatta ttttgccagt atcatcttca ttgtctttgt aaaattttca gaaacaagtt
      721 tataaaaata tacacctgtc gcagcattga gtcctgaatt aaattttcca tcccatacaa
      781 cgctgtgctg tcctggattt cttgattcat tatcaatcaa cgtccttact tcatttccca
      841 aaatatcata aaccttcatt gtaacttttt cctgtttagg taattcaaat tttatagtag
      901 ttacaggatt aaaaggattt ggataattct gagacagatc ataattttca ggaatagaag
      961 ttgtaatatt cgaaacagct accagtcccg gataatcaaa accatatctc agtactctta
     1021 cgatttgccc gccaggtgaa aaactccata ctatgttacc tgcagatgta acttcaaata
     1081 aattcccgct ggtcgattct gagatcattg tatttccgtt tgcaagtctt tgatttccac
     1141 caagatgatt tgaataaaaa cctgatgcac tgtagctcca ttcagtatct gccggtccga
     1201 aagctgatcc ggatgtatag ctgtaaaatc ctgcactgtc agtaggggct tgaaactcga
     1261 ctaccattga agaattagta ccttctctgt tattaaatgc cataatattt ccgccgcccg
     1321 gaagactatc cggtacccag acgctgttat gaactacatt aaatatctgt gaacccggag
     1381 ctctgtaatt tgacggtctt ccccatctgt ataaaatatc tccgcctttt ccgtatctgc
     1441 cgcctgtatg tcctgcagct tcagtagttg tagtactgtg atctatcaca tagacttcat
     1501 ccagctcatg tgatgaaatt aagatctgat caagtgcggc attgtagctg atcccattta
     1561 tatgcatcca gtctccggat ccggcgctgc ctacattgat gtctaataat tcaggatgat
     1621 cgccgaccac tccgtaattt gcttttgtcg gatcaaaatc ctgtatcaga tgatcccaga
     1681 aatgccactt ccatactatg gttcctccgt tagttcccga cggctgaact tcaataagat
     1741 gatccggcca tatgactgca ctgtgattaa gtcctgctgc aactgcatca gtagctgtct
     1801 ttacttccca ggcgatcaga agtacatttc cgtttggcat cggacaaatg tcgtgatgag
     1861 ttctatatgt attgctgctg taagtatatt cccagatcag tggtccgtcc catgaatatt
     1921 tttgaactac acctgctact gcaccgccgt tgattgtaga tccggtggaa agtgccggac
     1981 gcatcaggtc tccgtttctc aatagatata ctgaatatcc gccgcttctg gtatgtgtcc
     2041 agaattttac aacattatta ttaagatcca ttaaatttgt tcgggtagat gatgatgaat
     2101 ataatgtgta gcccgggtat gcttgtgaaa ataaatcacc tgtcatcgat aaaaaaatta
     2161 cgactaaaag tattgtgaga atttttcctg atttcattta atctaaattt ggttttttgt
     2221 aattaaaaaa ttttaaaata aattttattg ctgcatatta ttttacaaga agcattttct
     2281 ttgtaacact ttgatttggt gtttttaaaa tataaaaata cagtccgctt gaaagatcag
     2341 ctcctttaaa ttcaactgag taatttcctg ctgtctgctt ttcatttact aaagttgcta
     2401 cttcatttcc cagaacatca taaactttta gtgaaacatc attattcaca gagatctgat
     2461 aactgattat agtatttggg ttaaaaggat ttgggaaatt ctgatacaga ttaaaatcat
     2521 ttacggtctc attcccatta taaattccga cctcacagtt tattccaagt gtattcaact
     2581 gcgaagctac actgcttctt cttgcattta tataagattt tactcccgga attctgaagc
     2641 ctgaaatcac aagatcactt ataatgtttg tttcaaactg cgcattggaa aacatctttc
     2701 tcggatcttc cattacataa ggtctgataa tagttgtaat actgtctatc tttggaaata
     2761 attttgaaga agaaaaatta tcagtaaata tttcacagaa tgtcatcaga tactcatttc
     2821 taagctgaga attattaatg atctgaccga aaagcggtct gcctgtatca ctactgacat
     2881 aagttaccgg taatgtttct atatttgaca gaccaccggg taaagctccc atacttaagc
     2941 ttgcatccca gactatccag ttcattttat aatccggtgc aagtgagtaa atataaaaat
     3001 tccttccgtt ataaacatac gagtcaagat tgcccataag aatatcgaca gccattgcct
     3061 tataatatga gttaagattt accttagatg aaaaagtatt gtaagcatct gtagtatgat
     3121 ttattgaatc aataaaactt acaagtctgt cccatcccat cttactgtct gattttattt
     3181 catacctgtt agtataattt gaagtatccg caccatacca tctgaaatct gagacaagat
     3241 tactggtggc tccaaaagca tctactgctt taaattgatc tccgtctcca tctccaaagt
     3301 gagtttccag gaaatcaccg ttcaaatgtt cgacaagtga atataatgca aaaagagtat
     3361 cgtttattga aagtcggaca taatttgctc ttggcgctac tatgccgaca ctctggcaat
     3421 aatccagatg aattttttct ctgaggaatg acggatcatt ccagaaatta ttcagatgga
     3481 ctcctttgag accattccat ttttggccgc taacaaattc attaaagctt aatctgaatg
     3541 actttttatt atttggatga gtgtaagatg aattgccttt taagcggaca cctacattat
     3601 tgattatggt accgttagca attacagtag cagctaagaa ctgttcattc ccctgattat
     3661 agtaaattgt caacgaatcc cagtaatttg gctgtgaaaa aataatgttt acattgtgaa
     3721 cttgtatccc tgtaaaaaga ctgtctcctg caccctgaga aaatgctgac cggggaatta
     3781 taaatcccgc agcaaaaagc atcagaaatg aaaatactaa taagtttttt ttcattgaaa
     3841 aatttttatt aattatttaa aattttgtta gtggttatta tagaaaatta taaccggtat
     3901 taattccgtt attttactaa aattaatttt ttaatatcgg aaaaagtaat tcctttatca
     3961 cctgaaaatt ccatcctgta aaaataaact cctgactgaa gatcttttcc attaaaagta
     4021 gattgataat ttcctgcatt aatattgccg tcaactaaaa ctgagatctc ttttccaagt
     4081 acatcataca cttttaattt tacataacca tttaatggaa ttgaaaaact aatcttggta
     4141 tcaggattaa acgggtttgg ataattctga tccagcctga aattctctgc gattatatta
     4201 cctgtattta ttaataaagg tacatcctgt tcgtcaagtc tgagagggtt atcagctcca
     4261 cctgcagggt tgacagtgtt agtaccgctc ggccagatac cgtcgtcgtc agttctgaat
     4321 tttataacag acgcatctga aagactaagt acgttgactc ttttgactcc gcccggaagt
     4381 tcattcggaa taatagatcc ccatctgccg tctcttgaat ccacactgtc cttataatat
     4441 tttacaagat ttgtcaaaac tgttgtatca atcccgtcac tttctgcaaa taccatactc
     4501 agaggtcttc cgctgttact gacattatca tagatcgtaa tgatattttt tggagtagcc
     4561 agtgaaattc cataaagtcc tgtaccggaa tttgcatcgg atgatgtaga aaatgccaga
     4621 acttttattg aatcatttaa tgcaaatctg tataaagtag aaccactgcc tgcgctgtcc
     4681 agtgtgatgg tcggataaat atgattacct gcagtgaatc tggcatttcc tgtattaaca
     4741 aatccaaacc atcctgtata acttccgctg ccgtttgtta atatcgaatc atatcctcct
     4801 ggatttgaaa ggtttgtagc tgtactgtat ctgaaatcat taccgtgtat aaagagagga
     4861 ttaccggcac cggaatttgt cccgccgaag tcagtatata tacaggcatt taaataatat
     4921 ctataatatg aatttggtaa aagtccactg acagtagccc tgtaaacata aggcagtctt
     4981 gtacttgtac ctgatcccat atattgtggg actatatcac ctgtgaaatt tgactgcact
     5041 aactgtgact gaactacaga taatgatgtt aaaataaata atgtagtaaa gaaaattctt
     5101 ttcattcttg atttattttt aaaatgttta aataatttat tttataaatg tcatttttct
     5161 gacttctgaa aaacttcctg attctaattt ataaaaatac actccactgg aaaatcctga
     5221 tccttcaaaa ctcgtttcat aagttcccgg agtatgatgt tcattaaaaa gtactctgac
     5281 cacttttccg ctaagatcat atactgacat ttttacaata ccgcttttta aaatatcata
     5341 tttaattttt gtaacaggat taaaagggtt tggataattt tggtttaaat taaaatcagc
     5401 aggcacatta ctgctgatct gattaatccc gataatttcc gaaatgtttc ttttccataa
     5461 agcattagct ccggttccgg cgtaaaggtc atttgccgaa accatcagaa cagtaacata
     5521 ttgctcaccc agattctgat ttctcggaat ccagttttgt ccattatctg cagacatata
     5581 tactccaaag ccgtatgtac ctgcaaacaa atatcctgaa ctttgtgcga gtgaataaac
     5641 atccttatca ttcagcgatg tctgagtcca ggttgtacct gaattattgg aaatataaac
     5701 tccgttaaag cttactcctg ctgtaatgtt ggttccgttt accaataatg catttacaga
     5761 ttcattattg agtgaagtct gagtccaggt gacagcatta tccgttgaat aaaacactcc
     5821 attataagta ccggcgtata aagtatttcc tgactgcaca agtgcaagac agacttcgtg
     5881 aaaagaagtg ccgctccagt tataacctcc gtcagtcgaa atatgaaaac catatcctcc
     5941 gcctgcgaaa attgtacttc cgtttattgc aagtgaatat acaacttcat ttgtaaggga
     6001 agtacctgac caagtgatcc cattatcagt agatatctga attccattgt ccgcacctgc
     6061 aagaattcta ttgccgtttg caacgattga gggaattgag tttgtattaa gatttgtctg
     6121 agcccatgta tttcctgcgt cagcacttct gaatactccg ttttctcctg atcctgcaaa
     6181 aaatactccg ccattattta tcacagaaaa aactgacaga ttatgcagct cactttttgt
     6241 ccagtttgca ccactgtttt gagaagaata tatacctttc ttcgaacctg caagaagctg
     6301 gcttccgttt gtatttacac tttgaactat ctcattactt atgcctgcag aagcccagtc
     6361 tgcaccgttg tttgcagttg aatatacacc tgaagaagta cctgaaaaaa tagtatttcc
     6421 cgtttgtgaa aatgaataaa cgacagaatt attaaaagaa gactggctcc aagttgcacc
     6481 gctgtttgca gaataatata cacctgaatt aattgtacct gcacaaacat tatttccatt
     6541 aactgaaatt gcaagtacat ctttattatt taaagatgtc tgagtccatg cagcaccgtt
     6601 gtctgtggaa atataaacac cttttgcaat tccaacacct gcatacaaag tattcccgct
     6661 tgctgctatc gagtaaattt caaaagtatt cagtcccgac tgaatccagt ttacaccctg
     6721 atttacagat ttgtaaacac cttcgctcgt agcggcaaaa attgttccgt tgatttctgt
     6781 tatttcagaa acgacctgtg aactcataga ggattgctgc caggtatttc cattatcatt
     6841 ggagtaatat aatccgtaaa ggtttgtacc ggcaaaaata ttattaccgc ttatgtatat
     6901 agagtaaact gatttatcac ttaattcagt ttgctgccag ctggtaccgt tattgttgga
     6961 tttgtaaatg ccgtagagat ttgtacctgc aaaaatatca tttccgcttc ttgaaataca
     7021 gtaaacatct ccgccgttga tgcctgttga gttttgtatc cattgtgagt ttacggggaa
     7081 ggtaaaagta attgtgaaaa agaatagagc aatggtttta attaatttca tttaaggatt
     7141 taaaaattat aaaataaatt tttgttagaa aatgcggagt taaaaataat tgtacaaaat
     7201 taggtaaaca aatcagcatg aacaatctag ttattgtaaa caaatgtaac agggtatcga
     7261 ttaaataatt ggtgcaacat aatttataat ggaaaattcg gacaggattt attatctgtt
     7321 taaaaaaaaa atgaacagca aagaaatatt tcaaatagca agacaacacc cacaaacact
     7381 agtcattccc gcggatgcgg gaatctcaac tgaaacctga cattttttcc aaacttaacc
     7441 aaataaatcc ctttgtctgt ttcgacagta agtaaatacc aaatttcctt atagcttctc
     7501 ctaatattag attcctgcat tcgcatgaat cacaattatt ggagcatttg aaaaagaaag
     7561 aatctgtgaa agaatttttt ttctaaatat ttttgatgta agtcatataa cacagaatat
     7621 tttaacactg aaaggtttta aaacttttta ttaacaaatg attttatgtt tagctaattt
     7681 tacaaaatta aattatttta ttaaaaaaat catgaatcat ttcaaaacca tttctcttca
     7741 attcattttc gttctaatat ttacaataat gcataattcg ttttcatttt ctcaaacaga
     7801 tcttaatcaa aataaagatc agttgatttc taagaccaat gaattaaagg aaaagtttaa
     7861 gttcaatgcc gtcatcgcag gactttggaa aggcgaacag gaaattttca caattgctct
     7921 cggggaatca atgacaaact atcctgcaac aacagacatg catttcagga tcggcggtgt
     7981 gtcagaaatg ttttatggta ctcttgtaat gatacttgct gagcaggaaa aaattaatct
     8041 tgaagataaa atctcaaagt ttcttcccga tttaccaaat gccgataaag taacaattca
     8101 aatgctgatc aaaaatactg ccggttataa ggattatgtc tataataata aatttgtgga
     8161 tctgatatta aaatatcctt tcagatatat aacaagagaa gagataattt cttacggtac
     8221 tcctgacgat ctgaattttc cgccgggcac tgaacaaaaa tattctcata ctgaatttac
     8281 aattcttgct gaagtcattg aaagagcaac aggaaaaaaa atgtttgaac tttatgagga
     8341 aaatattttt aagcctttag gactgacaaa taccggttac agtaataatg ctaacctgcc
     8401 gtcacccgta ctgcattcct tcagctctga cagaaaagtt tatgaagatg ctactttctg
     8461 gagtccttca tgggcgggtg aatcgggacc actttattct aatttatttg atctggcaaa
     8521 atggggacct gtctttggga aaggaaagtt gctgaacgaa aattctttta atacattact
     8581 tagccgtttc gagaatacag gaaaaccgga tctttatttt gcatcaggat ttgtagttgc
     8641 caacggctgg tatctgcaaa atccaagcat gaacggatac agcggtggat acggttattt
     8701 ccctgaagag gaaattactt taatagttta ttctacagag actgctgata caccctctga
     8761 taaaaaggca tttgatattt ttaaagagct ggtaaagatg gtcacaccgg attcacagat
     8821 aaatttttaa ttaatgactt taaatatctt ggacagattt gaataaaata ataaatacct
     8881 aaattttaat tctttaattt gttaattcat ttacgtaatt ttgtaaagtt ttaaaatacg
     8941 gcacagagag atggcagagt ggttgaatgc ggcggtcttg aaaaccgttg tggcctcgcg
     9001 gtcaccgggg gttcgaatcc ctctctctct gcaaataaat acaatgaaat ttattttact
     9061 taaaataaat tttttactat tcaaaattta ctaagtaaag tccgcgaaag aatataaaaa
     9121 ttcaatccgg acttttttat ttttaaaacc ttaaaaatat ttatatgaaa aaattgttca
     9181 tttacagttt aatactattc attccgatta ttatcagcag ctgttcaggt gtgaataaaa
     9241 ccattgaaaa tgcacagcga ctgcagttca aattaggtaa ggtcgacggt cttgatattg
     9301 ccggagtaaa tctcagtaat gtaaaatctt taaatgatgt gaatatttta cagggagcaa
     9361 atttgttagc cgcttttgcg tcaggcagac tgccaactac attcactgta aacctcattg
     9421 caaaaaatcc agacacctat cctggcggtg atgcagaatc aacttcactg atcaaaagtc
     9481 tggactggag attactgatc gataataaaa atataattaa cggagcgatt actcagccga
     9541 tcactatacc cggagtcggt aaatctgcaa ctattcccat tccgatatca atagatctgc
     9601 tcgaactttt tgcaaacaac agctatgaaa atataattaa tcttgcactt gccatcggcg
     9661 gaaaatccgg taacagctca agacttacat taaaaataaa accaactatt gatacttttc
     9721 ttggaggtat ttcttatccg ggtgagattg atgtgattga taaagagttt aggtagtact
     9781 cttagcacag attattaatc tgtgtattat aatgtttaag tttattaaaa gagtttgt
//
LOCUS       DAORVQ010000121         9700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3769_length_9700_cov_5.671954, whole genome shotgun sequence.
ACCESSION   DAORVQ010000121 DAORVQ010000000
VERSION     DAORVQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_9700_cov_5.671954"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..3643)
                     /locus_tag="PLX80_09950"
     CDS             complement(11..3643)
                     /locus_tag="PLX80_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQY21152.1"
                     /translation="MAEKRNYSIGFDVGSTTVKAVVIDTEKDEIIWSDYQRHDTKQPE
                     KSLELLKAMERDLDLEGVTGMRCFITGSGGGNIGKHIGGKFVQEVNAVSLAVEKLYPA
                     THSVIELGGQDAKIIIFKPDEETGKKKKIPSMNDKCAGGTGAVIDKINAKLKIPAAEL
                     SNMGYNDLRLHPVAGKCGVFAETDINGLQKSGVPSDQLMASLFESIIQQNISVLTRGH
                     TLMPEVLLLGGPNCYIKGMKDCWKYNIPKIWEERKVILPQGVAPEDLIKVPDNAQYFA
                     AIGAVEYGKEEEDHIGVYTGWKDLEHYILVGREEEKKKMNGGNTTGLYATDNELAAFK
                     EKHKIEKFIPATFKPGEVVRGCIGIDGGSTSTKAALVSLDKEVLIKAYQLSKGNPIED
                     TIDILGNIQKQVEDQGATLEILGVGTTGYAKDILKDVLQADVALVETVAHTQAGLHFY
                     PETDVICDVGGQDIKIIILNQGRVVDFKLNTQCSAGNGYFLQSTAQGFGYTVEEFADK
                     AFEAKTFPKFGYGCAVFMQSDIVDFQRQGWRAEEIMAGLANVLPKNIWLYVSQIPNLA
                     KLGSTFVLQGGTQHNLAAVKTQVDFIEERFKGSGIEPKVFVHKHTGEAGAIGCGIEAA
                     RLYNNGKRTEFIGMERVSNILYKTTREESTRCYFCKNKCLRTFIDVKTENIAPDKIEE
                     EKKKFIELTVIEPEQIANPKSKVPMANGTQRLIIATCEKGTVEDVTDMRGIKQGIDDI
                     KKSNPNIIEEAAKNAWISYKPEIVAEPVSEPKGIFISKKQKDEYSDRLAKFELRSKLV
                     IGIPRILNMYQHTPFFTAYFESLGVKPGNFVYSEFTTEEMYKAGAKRGSIDPCFPSKV
                     AIPHIHNLLYVQNKKKQIDFIFSPIVDNMPSELEFAQAHTACPTITATVEAAKAAFIK
                     EGDLFEQFQTEYVNPFIQLFNMQMTARQMFECFENRLGLTEEENMNAVKEGYIAYNKF
                     INNHKEKSHEVLQRLEKEDKIGIVVLARPYHNDPGINHEILEEFQKLGYPVFYQDTLP
                     TDEETLDKLFGDEIKAGIIRTAFDIEDVWKNSYSENTSRKVWAAKYTARHPNLIALEL
                     SSFKCGHDAPIYTVIQEIVEDSGTPYFSFKDIDENKPTGSIKIRVETIGYFLKRYRED
                     IVMKKEKEKSIEENLKEFEMKIRKNLELQEKLNVMKKADPSIDRKNGSPTLVTVEEIK
                     ILESVE"
     gene            complement(3925..5718)
                     /locus_tag="PLX80_09955"
     CDS             complement(3925..5718)
                     /locus_tag="PLX80_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="activator of (R)-2-hydroxyglutaryl-CoA
                     dehydratase"
                     /protein_id="HQY21153.1"
                     /translation="METKTLIPDLIPMIDKDHSEKTDKEQLIQKMLMEERKRLEKENE
                     IDKKEINHFKRPFEKAFTKDQREGTTILFGGLTWKHEKLIKGALQGIGYKTDYIPVPD
                     KKAFQLGKEYGNNGQCNPTYFTVGNLVQYLQGLEEKGMSKEDIINNHVFFTAGACGPC
                     RFGMYEAEYRLALRNSGFDGFRVLLFQQTGGLSQNELEAGLEMNLDFFLGIVNAMMLG
                     DIINELGYNIRPFEVNEGETDRVMGEVIDIFYEKLKSKKYFDFNSTMGKFLQKIPASE
                     YFTKFIDQILGDYYLEAGEEAFNKFNSIKVDRTRLKPIVKITGEFWAQTTEGDGNFNM
                     FPFLEKEGAQLLVEPISTWIMYMIHQGQLVNEDQKGLNVEKDLDGKKSLKEKMKLMKE
                     YNSSKASLLLAEKIFEREYNRYRKTFNNIPHELVDQKLLKSLAHKFYNSRVEGGEGHL
                     EVGKSIYYTENNLCHMILSLKPFGCMPSTQSDGVQSAVSNFYKDMIFLPIETSGEGDV
                     NAHSRVQMALGEAKSKAKIEYKECLDKTGYGLDEIKAYVESNDELQKPFHKIGHRKGI
                     SGMGANFILDVADMMKKDGIKSVSDVKKEVA"
     gene            complement(5752..7077)
                     /gene="hpnE"
                     /locus_tag="PLX80_09960"
     CDS             complement(5752..7077)
                     /gene="hpnE"
                     /locus_tag="PLX80_09960"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HQY21154.1"
                     /translation="MNSGKSVTIIGAGIAGLSTAVFLTEKGFEVTILESSPKAGGRAY
                     SFFDPERKMFFDNGQHILAGWYENTFEYLKIIDSFKLLNFQKTLEVNFINTKREIFKL
                     KCTDDDPPMNLVKGLLKFKALNIIDKIYLKNINKILNSDENDFRIYSNTAELLKGIKQ
                     TSNLIKYFWEPLILAVFNTNPEKVAPALFVNVMKTGFEEGKDSNLVIPSVDLNTLLVN
                     NTLKYFKEKNVTFKTGSKVISIDISDKVNFIVTENGEKIVSDFYVTAVPFFTFKKLFD
                     EKIYSENNFEADKLRSSAIVSVHIFLNEDIPEAVIPDNSLGMTGLIGTTVQWIFLRNR
                     RHISLVISGADETGLTEKKSEEIFEICQSDLKKTIKDFDKLDISGYKVIKEKRATFIP
                     DRDSENFRPDQKTKYSNLFIAGDWTDTGLPSTIESAVKSAKIISEIINK"
     gene            complement(7074..7970)
                     /locus_tag="PLX80_09965"
     CDS             complement(7074..7970)
                     /locus_tag="PLX80_09965"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HQY21155.1"
                     /translation="MNSGIKEEKFTEPESNKRSSFFYSFSLLPKEKNEAINTIYAFCR
                     ETDDIVDETDTSTETKRSRILEWKSEFEIAIINEDSKFSLLNKVSSVMKKFKISSEPF
                     FDLIKGMEMDIAKNRYNTFGELNDYCYKVASTVGLMCIEIFGYRNPKTKDFAVNLGIA
                     LQLTNILRDIKTDAQLGRIYIPLEDLQKFNYSESELMNFNYNEEFRKLMKFECDRAHM
                     YYKKADESLDRSDKGLMLAARVMQHIYFRILKKIEKKNYNVFESELKVSKFKKIMITA
                     GIYLKYRLLYNISESDLAVNGK"
     gene            complement(7967..8842)
                     /gene="hpnC"
                     /locus_tag="PLX80_09970"
     CDS             complement(7967..8842)
                     /gene="hpnC"
                     /locus_tag="PLX80_09970"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HQY21156.1"
                     /translation="MQKEYESAYEYCRNIAVSHYENFPVGSVLIPKDKRKYVYAVYAF
                     SRYADDIADSTDLDAKIKLEKLEGLETELDKIENKSVNEFSIETKNIFLALFDTVNKL
                     NIPLSEFRNLLSAFKQDSVGKLYKNFDELLDYSNRSANPVGHLVLYVFGYDPENNYDA
                     FKYSDKVCTALQLANFWQDVSVDLNMDRIYIPEDIMMKNNYSAILLSEKVEDDDFRKT
                     LSELCDRTDEIFESGNEIIKFLNGRLKFEIKATINGGREILSKIRKINYNVLNNRVSL
                     SSVDKFKIFINAFIK"
     gene            complement(8899..9315)
                     /locus_tag="PLX80_09975"
     CDS             complement(8899..9315)
                     /locus_tag="PLX80_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21157.1"
                     /translation="MIYLLNKFLNLSFIAKTIWCIQNFLTMEFYRKICNNCYWSSVYS
                     DKHNVFKQSLLLVLSIIPVLFFSFFYSPTDPANLKRSSFFIKLANLFYGLKVCVKKYI
                     NFISNKLILLSGSLVDTERIKFKSPDIITLESSYLI"
BASE COUNT         2776 a         1995 c         1375 g         3554 t
ORIGIN      
        1 taactactaa ctattccact gactcaagaa ttttaatttc ttcaacagtc actagtgttg
       61 gactgccgtt ttttctgtca attgatggat cagctttttt cattacattc agcttttcct
      121 gaagctcaag attttttctt atcttcattt caaattcttt cagattttct tcaatggatt
      181 tctctttttc ttttttcatg acaatatcct ctctgtatct cttcagaaaa tatcctatag
      241 tctcaactct gatcttgatc gagccggttg gtttattttc atctatgtcc ttaaaagaaa
      301 aataaggtgt tccggaatcc tcgactattt cctgtatcac tgtatagatt ggagcatcat
      361 gtccgcattt aaaagaagac aattcaagtg cgatcagatt tggatgtcta gcagtatatt
      421 ttgcagccca tactttacgc gaagtatttt cggaatatga atttttccaa acatcttcaa
      481 tatcaaaagc agttctgata attccggctt tgatctcatc accgaataat ttatcaagtg
      541 tttcttcatc agtcggaaga gtgtcctgat aaaatacagg gtaacccagc ttttgaaatt
      601 cttctaatat ctcatgatta attcccggat cattatgata aggtctggca agcactacta
      661 ttccaatctt gtcttctttt tcaagtctct gtagcacttc atgagatttt tccttatgat
      721 tatttataaa tttattataa gcaatatatc cttcctttac cgcattcata ttttcttctt
      781 cagtaagtcc aagtctgttc tcaaaacatt caaacatctg tcttgctgtc atctgcatat
      841 tgaacagctg aataaaagga ttcacatatt cagtttgaaa ctgttcaaag agatctccct
      901 cttttataaa agcagctttc gcagcttcca ctgttgcagt gatagtcgga catgctgtgt
      961 gagcctgtgc gaactcaagc tcagaaggca tattatctac tatcggtgaa aatatgaaat
     1021 caatttgttt ctttttgttc tggacataaa gaagattatg aatatgaggg atcgcaactt
     1081 ttgaagggaa acaaggatca atgctgccgc gctttgcacc ggctttgtac atctcttccg
     1141 ttgtaaattc tgaataaaca aaattacccg gcttcacacc aagtgattca aaatatgcag
     1201 taaagaatgg tgtatgttga tacatattca ggatcctcgg aattccgatt actaactttg
     1261 atctcaattc aaattttgca agtctgtcgg aatattcatc cttttgtttt ttagaaatga
     1321 atataccttt tggttcagat acaggctcag caacgatctc aggcttgtat gatatccagg
     1381 cattttttgc tgcctcttct ataatattcg gattactttt tttaatatca tcaattccct
     1441 gcttgattcc tctcatatca gttacatcct ctacagtccc tttctcacag gttgcaatga
     1501 taagtctttg agtaccgttt gccattggaa cttttgattt tggatttgca atctgctcag
     1561 gctcaatgac ggtaagctct ataaatttct ttttttcttc ttctatctta tccggtgcaa
     1621 tgttttcagt ctttacgtca atgaaagttc ttaggcattt atttttacag aaataacatc
     1681 tggttgattc ctctcttgtt gttttatata aaatatttga gactctttcc attccgatga
     1741 attctgttct cttaccgtta ttatataatc tcgctgcttc aattccgcag cctatcgctc
     1801 ctgcttcacc ggtatgctta tgaacaaaga cctttggttc gataccgctg cctttaaatc
     1861 tttcctcaat aaaatctacc tgagttttta cagcagctaa attatgctga gtaccaccct
     1921 gcagaacaaa tgtagaacca agcttcgcaa gatttggaat ctgagaaaca tacagccaga
     1981 tattttttgg aagtacattt gcaagtccag ccatgatctc ttcagctctc catccctgtc
     2041 tctgaaaatc tactatatca gactgcataa atacagcaca gccgtatccg aattttggga
     2101 atgtctttgc ttcaaatgct ttatctgcaa attcctcgac agtatatcca aatccctgcg
     2161 cagttgactg taggaaatat ccattacctg ctgaacattg agtatttaat ttgaaatcaa
     2221 caacccttcc ctgattcaaa ataattatct taatgtcctg tccgccaaca tcacagatca
     2281 catctgtctc cggatagaaa tgaagtccag cctgtgtatg agcaactgtt tcaaccaatg
     2341 caacatcagc ctgcaataca tctttcagaa tatcttttgc ataacctgtc gtaccgacac
     2401 cgagaatttc aagagtagct ccctgatctt cgacttgttt ctgaatgttt ccaaggatat
     2461 caattgtatc ttcaatagga tttcctttcg aaagctgata cgcttttatt agaacttcct
     2521 tatccagtga aaccaatgca gcttttgtag acgtcgaccc gccgtcaatt cctatacatc
     2581 ctcttactac ttcacccggc ttaaaagtag caggtatgaa tttttcaatt ttatgttttt
     2641 ctttaaatgc tgctaattca ttatctgttg catataaacc tgttgtattt ccgccgttca
     2701 tttttttctt ttcctcttca cgaccaacta atatataatg ctcaagatct ttccatcctg
     2761 tatatacacc aatgtgatct tcttcttctt tgccatattc gacagcgcca atagctgcaa
     2821 aatattgagc gttatcagga actttgataa gatcttccgg agcaactcct tgaggaagga
     2881 taacttttct ttcttcccag atctttggaa tattatattt ccagcagtcc ttcatacctt
     2941 tgatataaca atttggtccg ccgagcaaaa gcacttcagg cattaaagta tgaccgcggg
     3001 ttaagacaga aatattctgc tgaataattg attcaaaaag tgaagccatt agctgatctg
     3061 aaggcacacc tgacttttgt aaaccattta tatcagtctc agcaaacact ccgcatttac
     3121 ccgctacagg atgaagtctc agatcattat atcccatgtt gctaagttct gctgcaggaa
     3181 ttttcagctt cgcatttatt ttatctatga ccgcaccagt accacctgcg catttatcat
     3241 tcattgacgg aatttttttc ttcttacccg tctcttcatc cggcttaaaa ataattatct
     3301 ttgcatcctg accaccaagt tcgatcacag aatgtgtggc aggataaagt ttttccactg
     3361 caagtgaaac tgcatttact tcctgcacaa attttccgcc aatgtgtttc ccgatatttc
     3421 caccgccgga tccggttata aagcatctca ttcctgttac gccttccaga tcaagatctc
     3481 tttccattgc tttcaaaagt tcaagtgatt tttccggctg cttggtatca tgccgctgat
     3541 aatccgacca gatgatctcg tctttttcag tatctatgac tacagctttt accgtagttg
     3601 aacctacgtc aaatccaatc gaataatttc ttttttccgc cattggttta gtttttattt
     3661 agttaaattt tttcatttat ttaaatacat ttatactctt ttatgaggat aaacttcttt
     3721 atttcttggt ctcgtctcaa ccaccttcac tccccctttg ttaaaggaga tggatctcta
     3781 aaaatattca ttatttattc tgttaatatt ttttttatat aacttctctt ttacttacaa
     3841 tttctatttt ctttcacaca ttacttctcc cccttaataa tagggaggtt gttgcgaaaa
     3901 ctatttaact atttctaatt tattctaagc aacttctttc ttaacatcac tcacactctt
     3961 tatcccatcc tttttcatca tgtcagctac atctaatatg aagtttgctc ccattccaga
     4021 gattcctttt ctgtgaccga tcttgtgaaa tggtttttgt aattcatcat tggattcgac
     4081 ataagctttt atttcatcaa gaccataacc ggttttatcc agacattcct tatattcgat
     4141 cttggctttt gactttgctt cacccagcgc catctgaact cttgaatgtg cattcacatc
     4201 tccctctcct gatgtttcga tcggaaggaa tatcatatct ttgtaaaaat tcgaaactgc
     4261 tgactgaaca ccgtctgact gagtcgaagg catgcaacca aacggcttta acgaaaggat
     4321 catatgacat aaattatttt ctgtgtagta aatactttta cctacttcta aatgtccttc
     4381 accgccttct actcttgaat tgtaaaattt gtgtgctagt gattttaata gtttctgatc
     4441 tacaagctcg tgaggaatat tattgaaggt ctttctgtag cggttatatt ctctttcaaa
     4501 aatcttttca gcaagtaaca gtgatgcctt actgctgttg tattctttca tcagtttcat
     4561 tttctctttg agtgattttt tgccatcgag atctttttct acattaagac ctttctgatc
     4621 ttcattcaca agctgaccct gatggatcat atacataatc catgttgaga tcggctctac
     4681 caaaagctgc gcaccttctt tttcaaggaa cgggaacata ttgaaatttc catcaccttc
     4741 agtcgtctgt gcccagaatt cacctgtgat ctttacgatc ggctttagtc ttgtcctgtc
     4801 gactttgatc gaattgaatt tattaaatgc ttcctctcct gcttctaaat aataatcacc
     4861 aagtatctga tctatgaatt ttgtaaaata ttctgatgca gggatctttt gtaaaaactt
     4921 tcccatcgtt gaattaaaat caaaatactt tttcgatttc agcttttcat aaaatatatc
     4981 tataacttct cccattactc tgtcagtctc accttcattg acttcaaagg gtctgatatt
     5041 atatccaagc tcattaataa tatctccgag catcattgca tttacgattc caaggaaaaa
     5101 atcaagattc atttccagtc cggcttctag ttcattttgt gaaagtcctc ctgtctgctg
     5161 aaacagcagt actctgaatc cgtcgaaccc tgaatttctc agtgcaagtc tgtactcagc
     5221 ttcatacatt ccaaatctgc acggtccgca agctcctgct gtaaaaaata catggttgtt
     5281 aattatatct tctttactca tacctttttc ttcaagaccc tgcaaatact gcacgagatt
     5341 tccaacagta aaataagtcg ggttacactg tccattatta ccgtattctt ttccaagctg
     5401 aaatgctttc ttatccggca cgggaatgta atcggttttg tatccgatac cctgaagtgc
     5461 tcctttaatc agcttttcat gcttccatgt cagtccgcca aagagaattg ttgtaccctc
     5521 tctctgatct tttgtaaatg ctttttcaaa aggtctctta aaatgattga tctctttttt
     5581 gtcaatttca ttttctttct caagcctttt tctttcttcc attagcattt tctgaatcag
     5641 ctgctccttg tctgtctttt ccgaatgatc tttatcgatc attggaatta aatccgggat
     5701 caaagttttg gtttccatga gttttttttt aatgattaaa aaatatttta attatttatt
     5761 aattatttca cttattatct ttgcactctt tactgcgctt tcaattgtcg aaggcaaccc
     5821 ggtatctgtc cagtcacctg ctataaataa attactgtat tttgttttct gatcaggtct
     5881 gaaattttca ctgtctctgt caggtataaa agttgcccgc ttctctttga tcactttata
     5941 tcctgatata tcaagtttat caaaatcttt tatcgtcttt tttaaatcac tttgacaaat
     6001 ttcaaatatc tcttcagatt tcttttctgt cagacctgtt tcatctgcac cgctgatcac
     6061 aagacttata tgtctcctgt ttctcaaaaa tatccactga actgtagtac cgataagccc
     6121 tgtcatacca agtgaattat ccggtatcac cgcctcaggt atatcctcat tcagaaaaat
     6181 atgaaccgaa actatcgcag acgatcttaa tttatccgct tcgaaattat tttccgaata
     6241 aatcttttca tcaaacagtt tcttaaaagt aaagaacggt accgctgtta cataaaaatc
     6301 tgaaactatt ttctcaccgt tttctgtaac aatgaaattc actttgtcac ttatatcaat
     6361 tgaaattact ttacttcctg ttttaaatgt cacatttttt tctttaaaat acttcagagt
     6421 attattgaca agcaaagtat ttagatcgac tgaaggtatt acaaggttcg aatctttccc
     6481 ctcttcaaaa ccggtcttca tcacattgac aaaaagtgcc ggtgcaacct tctcaggatt
     6541 tgtattaaat actgcaagta tcagcggctc ccagaaatac tttatcaggt tacttgtctg
     6601 cttaattcct ttcagcagtt cagccgtgtt tgaataaatt ctaaaatcat tttcatcact
     6661 gttcagaatc ttattaatat ttttcagata aattttatca ataatattca gtgctttaaa
     6721 tttcaaaagc cctttcacaa gattcatcgg cggatcatca tctgtacatt ttaatttaaa
     6781 tatctctctt ttagtgttaa taaaattcac ttcaagagtt ttctgaaaat tcagaagttt
     6841 aaagctgtct attattttca gatattcaaa agtattttca taccatcctg ccagtatgtg
     6901 ctgcccgttg tcaaagaaca tttttctttc cggatcaaaa aagctgtaag ctcttccgcc
     6961 ggctttcgga gatgattcaa gtatcgttac ttcgaatcct ttttcagtca gaaatactgc
     7021 cgtcgataaa ccggctattc ctgctccaat tattgttact gactttccgg agttcattta
     7081 ccgttcactg ctaaatcact ttcactgata ttatataaaa gtctgtattt caaatatata
     7141 cctgcagtga tcattatttt tttaaacttc gaaactttta attcactttc gaaaacgttg
     7201 taattctttt tctcgatctt ttttaatatc ctgaaataaa tatgctgcat tactcttgct
     7261 gccagcataa ggcctttatc ggatctgtcc aaagattcat cagctttttt ataatacatg
     7321 tgtgccctgt cgcattcaaa cttcatcagt tttcggaatt cttcattata attaaaattc
     7381 ataagttcgc tttctgaata attgaatttc tgtagatcct ctaaaggaat atagatccta
     7441 ccaagctgtg cgtcagtttt tatatccctt aaaatatttg tcagctgtag agcaattcca
     7501 agattaactg caaaatcttt tgtcttagga tttctgtatc caaatatctc tatacacatt
     7561 aatcccactg tcgatgcaac tttgtaacaa tagtcattta attcaccgaa tgtattgtat
     7621 cggtttttag caatatccat ctccattcct tttataagat caaaaaaagg ttcggatgaa
     7681 attttaaatt tcttcatcac actgctgacc ttatttaaca gtgaaaattt tgagtcttca
     7741 tttataattg ctatctcaaa ttcagatttc cactcaagga tcctggatct ctttgtctca
     7801 gtgcttgtgt cagtctcatc cactatgtca tccgtttcac ggcaaaaagc atagatagta
     7861 tttatagctt cattcttttc ttttggcagc agtgaaaatg aataaaaaaa acttgatctt
     7921 ttattagatt caggctctgt aaacttttct tctttaattc cgctgttcat tttataaaag
     7981 cattaatgaa tattttaaac ttatccacgc tgctcaatga aaccctgtta tttaacacgt
     8041 tataatttat ctttcttatc ttgcttaaga tctctctgcc gccgtttatt gttgccttta
     8101 tttcaaattt caatctgccg tttaagaact ttataatttc attgcctgat tcaaatatct
     8161 catcagttct gtcacagagt tcagaaagtg tttttctgaa atcatcatcc tcaacttttt
     8221 ctgaaagtaa tatcgccgaa taattatttt tcatcataat atcttcagga atatagatcc
     8281 tgtccatatt cagatctaca gaaacatcct gccagaaatt tgcaagctga agcgctgtac
     8341 agactttatc agaatattta aatgcatcat aattattctc cggatcatat ccaaacacat
     8401 acaagaccag gtgtccaaca ggatttgcag atctattgga atagtcaagt aactcgtcaa
     8461 aatttttata cagctttccg actgagtcct gcttaaaagc gctgagtaaa tttctgaatt
     8521 cacttaaagg aatattcagt ttatttacag tatcaaaaag agcgagaaaa atattctttg
     8581 tttcaataga aaattcattt acacttttat tttctatctt atcaagttcc gtttcaagac
     8641 cttctaattt ttctaatttt atctttgcat caagatcagt ggaatcagct atatcatcgg
     8701 catatcgtga aaatgcataa acagcataaa catattttct tttatctttt gggatcaaga
     8761 ccgatccaac cggaaaattt tcataatgac ttactgcaat atttctgcag tattcataag
     8821 cagattcata ctctttttgc atctcgaaat taaactaatc ggaaattgat cttaaagata
     8881 tgagaaatag gatagatcct aaatcagata ggatgactct aatgttatta tatcgggtga
     8941 tttgaatttt atccgttctg tatcaacaag tgaaccggat aaaagaatta atttattgga
     9001 aataaaatta atatattttt ttacgcaaac ttttaatccg taaaataaat ttgccagctt
     9061 aataaaaaaa gatgatcttt ttaaattagc cggatcagtg ggagaataga agaatgaaaa
     9121 aaataagaca ggaatgatcg ataatacaag taacaaggac tgtttgaaaa cattatgttt
     9181 atctgaataa acactgctcc agtaacagtt gttacatatc ttcctgtaaa attccattgt
     9241 taaaaaattc tgaatgcacc agatagtttt ggctatgaaa gataaattca gaaatttatt
     9301 taacagataa atcataaatt tgatttttgt aaaaactaat ttttctaatc ttaaatgtca
     9361 agattcattt ttgagtattt tttattttcc agcattagag tttttactat caggtctcaa
     9421 tgacacagat aattttaaat acaagtttaa agattctctt tttaaaaaat aaaaaaaccc
     9481 tccaaacata tctttagata taatatgaag ggttttaaaa aatattttct taactatttt
     9541 ctacaatttt acttcaccat caccatacga ttcacctgtc tgaaatcacc cgcttctatc
     9601 ttataaaaat acactccgct ggagagccct gtgccgttga attctattga atatattccg
     9661 gcagtacgga attcattatt gacaagtctt ttgatctccc
//
LOCUS       DAORVQ010000122         9692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3777_length_9692_cov_5.485732, whole genome shotgun sequence.
ACCESSION   DAORVQ010000122 DAORVQ010000000
VERSION     DAORVQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3777_length_9692_cov_5.485732"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1173)
                     /gene="rsgA"
                     /locus_tag="PLX80_09980"
     CDS             complement(100..1173)
                     /gene="rsgA"
                     /locus_tag="PLX80_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQY21158.1"
                     /translation="MTIFTNQSKIGWNDFFEDQLNESDKELTPGRVIICNKNNYLIST
                     GDAEITGEVSGKFHFNAESKNDFPAVGDWVLLRNFEEYDNAIIERVLKRQSKLSRNSA
                     GLKHEEQILASNIDTVFIISSLNQDINLRRLERYISLIIDSKLIPVVILSKSDLCENT
                     EEKLSEVKTISGIRDIFIVSALNNIGMNELQKYFYGNKTVAVIGSSGVGKSTLINSLI
                     GEYLMNVSDISLYKDKGRHTTSHRELIFLNDGGAIIDTPGMRELQLWEGSEGLTEAFP
                     DIEKYIGQCKYSNCEHDTEEGCAIKEAIEKGEFDELRFKSYKKLEREITRFENKTNKK
                     ALLKEKKKWKKLTHEHRRSQHKK"
     gene            complement(1348..3186)
                     /locus_tag="PLX80_09985"
     CDS             complement(1348..3186)
                     /locus_tag="PLX80_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21159.1"
                     /translation="MSKLKERQFSSKGELIPFFKRLFTYGLKYKKWFSGFIIFVLGVA
                     AVEAVFPLIWLSLLDNVIVPSIESNKVQIQNGLQGNSDLSGLTYYVTIIFSLGAALSG
                     SVFLFIHFGGKIQEYIMFDIRQDLFKKLQELTFSFYDKAAVGWLMSRITSDTIKVTEL
                     ISWGLIEFIWGIGMIVFCITAMLFYDVRLAMIVILTIPFLILISFKIRKLILKYSRES
                     RKFNSEITASYNEHIVGVEVNKSMVQENRVSDEFDSLSDKMRVSSYKSAYFSALYQPL
                     VIFGGSMAAALIIYYGGSMAITFPPEISVGMLAAFFGYATLIYEPILDISRFYSQAQN
                     CISAGERIFSLLDTKPAINDKEDATDFIEIEGEITFENVLFYYEKNKPVLENMDLRIE
                     KGQSIALVGETGGGKSSIINLICRFYEPTAGRILIDGEDYTDKTTTSLRNKLGVVLQT
                     PHLFSGTIRENIKYGRDDADENEIVNSLLLIGGDEFIERLDEEVGEGGEKLSLGEKQM
                     ISFARAILADPRIFIMDEATSSIDTLAEAKIQKGIHELIKGRTSIIIAHRLSTIKNCD
                     RIIVIKKGKIIEDGSHKVLMLAKGNYYNLYTRQLREQRENEMLTKI"
     gene            complement(3238..5091)
                     /locus_tag="PLX80_09990"
     CDS             complement(3238..5091)
                     /locus_tag="PLX80_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21160.1"
                     /translation="MNNIKRLFQILGEWKWFYVLSGILLIFAMFIRMLEPKILQITVD
                     KVIVFYQTGGKVEQVPDDSFTVFLYSILPEFKIENPEVILISLGILFLVISLMRGIFS
                     FSSSAITASSTEKATKNLRLRLFSHIQALPLAYHSKTPTGELVQRCTGDVETLRKFAA
                     TQVVEVIRMAAIFAGAFIMMASINLNYAFVSIIFVPVIFIGTILFFKKESQIWTEHEL
                     RQDKLSIIVQENLSGIRVVKAFAKENFEIDKFTKQNLEKRHWGLYLNKLHRIFWPSAD
                     FLVHTQIAISIFYGGYLALNGQLTVGEYMAFYSYSMFVTWPMRRLGQLISEMGMATVA
                     INRIFSILETEKENYEGKNFSEKKLNGNIEFQNVSFNYEGSEKKRVLNNISFKVKAGE
                     KIALLGPTGAGKTTIISQMLRFFDPDSGKIFIDGIESEKYSKEYLRSRFGVVLQKPFL
                     FSTTIRDNIAYSKPDSHIDEVIESARAARIHEIITEVFPDSYETVVGEKGVTLSGGQK
                     QRVAIARTLLKDPDILILDDSTSAIDSETEFEIQQQLRKLMKGKTTFIIAHRITSIQD
                     CDRIIVMDKGCILEEGTHEELVKNNGFYKKILDIQVSIEEEIDAEVETIKN"
     gene            5563..8358
                     /locus_tag="PLX80_09995"
     CDS             5563..8358
                     /locus_tag="PLX80_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21161.1"
                     /translation="MKKLFSIFTFFTVSLFYLNASSQLSGTLSIPGNYATIKLAIEDL
                     NTQGVGIGGVVFNVAAGHTETSSNMTISILANQPTSANTVVFQKSGAGANPLITAAAG
                     ISATADGIIKFSGADYITFNAIDLLDPNTNTGNGVMEWGYALMRASTTDGSQNNVIKN
                     CVITLQKISSLSYGIFVANRDTNGATVVASDPDGQNSYNKLFGNTISNVYKGIVTISA
                     STARDIDNQIGVNSENPNQITNWGGSTIASEGIRAEGQVNLKIVNNIVNGGNGTANAV
                     VGIIATLFGTTALAPNYEISHNTVTVNSNQSSSATYGIRALATADTIRMHNNIIENCV
                     TNYTGTATFNAMVHDGVGLSNAAYINDNIVRNNSHTGTGTSTLIGCSSDMNFLELSSN
                     EVYGNTRTSISGTMNCLLAADAATLNCYSNEVYNNSIPNTSGTSASNLYGYINSGNPG
                     TENIYNNIIRNLTVSGSNTAGGALTVGIRSNAAVTTTKNIYGNLIHSLSAVSGNFTTG
                     GIFGIYSTNGASHNIYSNRIYNITNNSGSGTSGGCWVSTGTSFTIYNNFISDIKAPNS
                     TQANGVVGIHSSTTTANSNIGIYYNSIYLTASGGSTFGSSGISVNGNATPTTANLDLR
                     NNLVINLSTPGSSGGVTTALRRSLATLSNFSELSDNNDLYSGTPASNRLIYFDGTGYQ
                     TINDYKNAVTPRESNSNSVSVNFVNPATGDLHLTGASVQNPNLAGMPISGISNDIDGD
                     ARNFKYPYKGADESTAFNIQTLNLTVNLEACSPVQDTLTVSLRSTVSPYGIVATDKRY
                     LSPAGSAAFIFINASNGVNYYIVANHRNSIETWSKNGGEVFTAGSLTYDFTTAASQAF
                     GNNQVLVSGEYSIYTGDVNQDEVVDVTDLGLIDNDASNFVTGYVDTDLNYDNVVDVTD
                     ASFADNNAAGFVSVSKP"
     gene            8468..8974
                     /locus_tag="PLX80_10000"
     CDS             8468..8974
                     /locus_tag="PLX80_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21162.1"
                     /translation="MKSLNLLLVVVLFLTTAIVGAQTDDVKIQTNSDVSSEKLTESIA
                     DQPGLDGLMTELVNGINVSAFKDGETGQGEVLKMLSGLGAEDINKYGLALGSLAGALN
                     SSAFLPTWTENEKGIMSQLQNAVSISDVAGAVLEMSGMIDRSSFTEGFVENISSWQNS
                     LESISMTK"
     gene            9099..9596
                     /locus_tag="PLX80_10005"
     CDS             9099..9596
                     /locus_tag="PLX80_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21163.1"
                     /translation="MKKLFNIFLFLIFIISGIAGAQTDNSKGDTKKKSKSNDFLNSQT
                     GLNSLMGQFVKGINNSSFSDGKTGKNEVLGMLKGVNTSDYLKYAGIVGSLAGALKGTS
                     FLPDWASNESGVLDQLQNAASIADVAGGLLEMSSMLDPSSFTKSFSKKKSSWSSALNV
                     LSMLK"
BASE COUNT         3105 a         1744 c         1531 g         3312 t
ORIGIN      
        1 aaaatttttt tcaatatttt tattcaaatt tatactctcc aagataacta tttatttatc
       61 ttaagttaca aattgcaatt taaaatttaa aatttaaaat tatttcttat gctgtgatct
      121 tctatgttca tgcgttaatt ttttccactt ctttttttct tttagcaatg cttttttgtt
      181 tgttttattt tcaaatcttg ttatttcgcg ttcaagtttt ttataacttt taaaacgtaa
      241 ttcatcaaat tctccttttt caattgcttc ttttatagca catccttctt cagtatcatg
      301 ttcgcagttg gaatatttac attgtccgat atatttttca atgtctggaa atgcttcagt
      361 aagaccttca ctgccttccc acaattggag ttcacgcata cctggagtgt ctataattgc
      421 cccgccgtca ttaagaaaga tcagttcacg gtgtgaagtc gtatgtcgac ctttatcttt
      481 atataagcta atatcactta cattcattag atattctccg atcagactgt taataagcgt
      541 tgattttccg acacctgacg agccaataac agccactgtt ttatttccat aaaaatattt
      601 ttgtagttca ttcatgccaa tattattcag agcacttaca ataaatatat ctcttattcc
      661 tgaaattgtt ttcacctctg ataatttttc ttcagtattt tcacacaggt ctgacttact
      721 taatatcact acaggtataa gcttgctgtc aataataagt gaaatatatc tttcaagcct
      781 ccgcagattt atgtcctgat ttaaagatga aataataaat acagtatcaa tattagatgc
      841 aagtatttgc tcttcgtgtt tgagacctgc tgaatttctc gaaagcttgc tctgtctttt
      901 aagaactctc tcgataatgg cattatcata ttcctcaaaa tttctgagaa gcacccagtc
      961 tcctacagca ggaaagtcat ttttactttc agcattgaaa tgaaatttac ctgatacttc
     1021 acctgtaatt tctgcatcac ctgtgctgat cagataatta tttttattgc aaattataac
     1081 tcttccgggt gtaagctctt tatccgattc atttagctga tcttcaaaaa aatcattcca
     1141 cccgatctta ctttgatttg taaatattgt caaattatta attttatttt gttaattaac
     1201 atttttaaaa actcatattt tggaaaagta gatcttaaac attgatttaa ataattcagt
     1261 taaatagcat tactttaaat aacttagatg attatcgagt ttaattaaaa tttgatgtaa
     1321 cttaatataa agagatgtta ttaacattta tattttagtc agcatttcat tttctctctg
     1381 ctctctaagc tgtcttgtgt aaagattata ataatttcct tttgctaaca taagaacttt
     1441 atggcttccg tcttcgatta tcttgccttt ttttattaca ataatcctgt cacaattttt
     1501 aattgtagaa agtctgtgag caataataat ggaggtcctg ccttttataa gttcatgaat
     1561 tcctttttga atctttgctt cagcaagagt atcaattgat gaagtagctt catccataat
     1621 aaaaattctc ggatcggcta gtattgctct ggcaaaagag atcatctgct tttctccaag
     1681 ggaaagtttt tcaccgcctt ctccaacttc ttcatcaagt ctttctataa actcatctcc
     1741 tcctatgagc aataaagaat taactatctc attttcatct gcatcatctc tgccatattt
     1801 tatattctct cttattgttc ctgaaaataa gtgaggagtc tgaagtacaa ctccaagttt
     1861 attccgaaga ctggtagtag ttttatctgt atagtcttca ccgtcaatga gaatccttcc
     1921 tgctgtcggt tcataaaatc tgcagataag atttatgata gaagacttgc cgccgcctgt
     1981 ctctccaact aaagcaatcg attgaccttt ttcaattctc agatccatat tttctaaaac
     2041 cggtttattc ttttcataat aaaataatac attttcaaaa gtgatctcac cttctatctc
     2101 aataaaatcc gttgcatctt ctttatcatt gatagccggt ttggtatcga gcagtgaaaa
     2161 aattctttca cctgctgaga tacaattttg cgcctgcgaa taaaatcttg aaatatcaag
     2221 tatcggttca tagataagtg tagcatatcc aaaaaatgca gcaagcatac cgactgagat
     2281 ctcaggcgga aaagttattg ccatacttcc gccataataa attatcaaag ctgcagccat
     2341 actgcctccg aatatcacaa gaggctgata caaagcggaa aaatatgcag acttatatga
     2401 agagacacgc attttatcgc ttaatgaatc aaactcatcg gagacacgat tttcctgtac
     2461 catgctttta tttacttcca ctccgactat gtgctcattg taacttgcag ttatctcact
     2521 gttgaatttt ctagactctc ttgaatattt caaaataagt ttcctgatct taaatgagat
     2581 caatattaaa aatggaatgg tcagaattac aatcattgca agccttacat cataaaagag
     2641 cattgcagtg atacagaata ctatcatccc gattccccat ataaattcga tcagtcccca
     2701 tgagatcagt tctgttactt ttattgtgtc agatgttatc cttgacatca gccagcccac
     2761 agcagcttta tcataaaatg aaaatgtaag ctcctgcagt tttttaaaca ggtcttgtct
     2821 gatatcaaac attatgtact cctgtatctt gcctccgaaa tgaataaata aaaatactga
     2881 tcccgaaagt gctgctccaa gcgaaaatat aatagttaca taataagtca gtccgctcag
     2941 atctgaattt ccttgcagcc cgttctgaat ttgtaccttg ttgctttcaa tggaaggcac
     3001 tatcacatta tccaaaagac tcaaccatat caaaggaaat accgcttcga ccgctgccac
     3061 tcccagcaca aagataataa atccgctgaa ccattttttg tatttcagtc cgtatgtgaa
     3121 cagccttttg aaaaatggga ttaactcacc tttactacta aactgccttt cttttaactt
     3181 actcacgatt caattttaaa ttttaaataa aatatattaa atattatttt taaattccta
     3241 attcttgatt gtttctactt ctgcatcaat ttcctcttca atcgaaactt gtatatcaag
     3301 aattttttta taaaagccgt tattttttac aagctcttca tgcgtacctt cctccagaat
     3361 gcatccttta tccattacaa ttatcctgtc acaatcctgt atggaagtga ttctgtgagc
     3421 aattataaat gttgttttac ccttcatgag ttttcggagc tgttgctgta tttcaaattc
     3481 agtttcggaa tcaattgcag aagtagagtc atccaagatc aaaatatcag gatctttgag
     3541 cagggttctg gcgatggcaa ctctctgctt ctgaccgcct gaaagagtca cacctttttc
     3601 tccgactact gtttcatatg aatcaggaaa tacttctgta ataatttcat ggatccttgc
     3661 agctcttgct gattcaatga cttcgtctat atgtgaatca ggttttgagt atgcaatatt
     3721 atctcttatg gtagtagaga ataaaaacgg tttctgaagc actacaccaa atctgctccg
     3781 tagatattct tttgaatatt tttcagattc aattccgtct ataaatatct taccgctgtc
     3841 cggatcaaag aatctcagca tttgtgaaat gattgttgtc ttaccagcgc cagtgggtcc
     3901 gagcaatgca atcttctcac cggcttttac tttgaagctt atattattaa gcactctttt
     3961 cttttcactg ccttcataat taaatgaaac attctgaaat tcaatattac cgttcagttt
     4021 tttttctgaa aaattctttc cttcgtaatt ttctttttcc gtttcaagga ttgagaaaat
     4081 tcggtttatc gctacagttg ccattcccat ttcacttatc agctgcccta atcttctcat
     4141 tggccaggta acaaacattg aataagaata aaaagccata tactcaccta cagtcaattg
     4201 accatttaat gccagatacc caccataaaa aatggatatt gcaatctgtg tgtgcacaag
     4261 aaaatcagcc gatggccaga atattctgtg aagtttattc agatataatc cccaatgtct
     4321 tttttcaaga ttctgttttg tgaatttatc gatctcaaaa ttttcttttg caaaagcttt
     4381 tactactcta ataccggata aattttcctg cacaattatt gaaagtttat cctgtctcag
     4441 ctcatgctct gtccagatct gactttcctt tttaaagaat aaaattgtcc caatgaaaat
     4501 aacaggtaca aatattattg aaacaaatgc ataattcaaa tttattgaag ccatcatgat
     4561 aaatgcaccg gcgaatatag ctgccattct aatgacttct actacctgtg tagctgcaaa
     4621 tttcctcaga gtctcaacat cacctgtgca cctttgcacg agttcgccgg tcggtgtctt
     4681 cgaatgataa gcaagaggta atgcctgtat atgtgaaaat aatctcagcc gcagattctt
     4741 tgtagccttc tctgtggaag atgctgtgat cgcacttgat gaaaatgaaa atatacctct
     4801 cattagtgaa atgactaaaa ataaaatacc gagtgagatc aatataactt caggattttc
     4861 aattttaaac tccggtaaaa ttgaatataa aaatactgta aatgaatcat ccggcacttg
     4921 ctcaactttt ccgcctgtct gataaaaaac aattacctta tcaacagtga tctgtagtat
     4981 tttcggctcg agcatcctga taaacattgc aaagatcaga agtattcctg aaagtacata
     5041 gaaccacttc cattcaccga gaatctgaaa taatctttta atattgttca ttgctattat
     5101 ttattaaact taatttaatt taaaaacctg ttttaaattt tgcataaaaa aaaaccgcga
     5161 atattttata tccgcggttt ttaaaaatct tcttttactt cagaggttaa tctgtttccc
     5221 tccggttcaa ccacggatat actgatgtat ccacaaaagt gaataaaggt gcaataatgc
     5281 ctgccatcgg tatgtatatt tttttattca gcattattta ctaattagac acctttcagt
     5341 ttttgattta aaatattcgt gatttgcgtt tacaaaatta cgaatgttaa ttttaaaaag
     5401 caaatttttt tttaaaaaag atcataaaaa aaattcttca ttgaaattca ttgcattggg
     5461 actgctataa ttccttctta aaaaactatt gaaatataaa ttcaattaac ttatttttaa
     5521 aaaattcttc aaactacttt tattaatttc aaaaaaaatc ttatgaaaaa gttattttcc
     5581 atttttacct tttttacagt ttcattgttt taccttaatg catcttcaca gttatcaggt
     5641 acattatcaa tacccggaaa ttatgcaaca ataaaactgg ctattgaaga tcttaatact
     5701 cagggagtag ggatcggcgg agtagtattt aatgttgcag caggtcatac cgagacttca
     5761 tcaaatatga ctatttcaat tttagcaaat cagcctacat cagcaaacac agttgtattt
     5821 caaaaaagcg gtgcaggagc taatccgctt atcactgctg ctgcaggaat atctgcgact
     5881 gctgacggaa ttattaaatt ttcaggtgct gactatatta cctttaatgc tattgatctg
     5941 cttgacccta atacaaatac cggcaacggt gtcatggaat ggggatatgc attgatgaga
     6001 gcttccacta ctgacggttc gcagaataat gtaattaaaa actgcgtcat cacattacag
     6061 aaaattagtt ctctttctta tggaattttc gtggctaaca gagatactaa tggagcgact
     6121 gttgtagcat cagatcctga cggtcaaaac agctacaata aattatttgg taatactatt
     6181 tcaaatgttt acaaaggaat tgtaactatc tctgcatcca ctgcaaggga tattgataat
     6241 cagattggag ttaacagtga aaatccaaat caaattacaa actggggcgg aagcaccatt
     6301 gcttcagaag gaattagagc tgaaggtcag gtaaatttaa aaatagttaa taatatagta
     6361 aacggaggaa acggaacagc taatgcagtc gtcggtataa tagcaactct gtttggtact
     6421 actgcacttg ctcctaatta tgaaatcagt cacaacacag ttactgtaaa ttccaatcag
     6481 tcctcgagtg caacttacgg tatcagagca ttagccacag ccgatacgat cagaatgcat
     6541 aataatatta tagaaaattg cgttacaaac tataccggta cagccacttt taatgctatg
     6601 gttcatgatg gtgttggctt atcaaacgcc gcatacataa atgataatat tgtaagaaat
     6661 aattctcata ctggtaccgg tacttctact ttaatcggat gttcatctga tatgaatttt
     6721 ctggagctaa gctcaaatga agtctatgga aatacacgaa ccagtatttc aggaacaatg
     6781 aattgtcttc ttgctgcaga tgctgcaaca ttgaattgtt attcaaatga agtttataat
     6841 aattctattc caaacacttc aggtacatct gcttcaaatc tatatggtta tataaattca
     6901 ggaaatcccg gtactgaaaa tatttacaat aatattattc gtaatttaac cgtaagcgga
     6961 agcaatacag ctggaggtgc attaacagta ggaataagat caaacgctgc agttaccaca
     7021 actaaaaata tctatggcaa tttaattcac agcctcagtg ctgtatcggg aaatttcaca
     7081 acaggaggta ttttcggaat atacagtaca aacggggcat cacataatat ttacagtaac
     7141 aggatttaca atattacaaa taattctgga tcaggaacat caggtggatg ctgggtttct
     7201 accggaacat cattcacgat ttataataat ttcatttccg atataaaagc accaaattca
     7261 acacaagcaa atggagtagt cggaattcac tcatctacaa caactgcaaa cagcaatatc
     7321 ggaatttatt acaattcaat atatcttaca gcttcaggag gttctacttt cggctcttcc
     7381 ggaatttccg ttaacggaaa cgctactccc actacagcaa atttagatct gagaaataat
     7441 cttgttataa atctttccac cccgggatct tccggcggag taactacagc gctcagaagg
     7501 agtttggcta cactttcaaa tttctcagag ttatctgaca acaatgattt atattcaggc
     7561 acacctgcat caaacagact tatatatttt gacggtacag gatatcaaac tattaatgat
     7621 tataagaacg cagttacacc cagagaatca aattctaatt ctgtttctgt caattttgta
     7681 aatccggcaa ccggagatct tcaccttaca ggagcttctg tccagaatcc gaatttagca
     7741 ggcatgccaa tctccggaat ttcaaatgat atagacggtg atgcaagaaa ttttaaatat
     7801 ccatataaag gtgccgatga aagtacagca tttaatattc agacattaaa tctaacagta
     7861 aatttggaag cttgttcacc agttcaggat acattaacag tttctctcag atctacagta
     7921 tcaccttatg gaattgtagc taccgataaa agatatctta gtcctgccgg cagtgcagct
     7981 tttattttta taaatgcctc aaatggcgta aactattata ttgtcgcaaa tcacagaaat
     8041 tctatagaga catggagtaa aaatggcggt gaagtattta cggccggatc tttaacatat
     8101 gattttacta cagctgcgtc tcaggctttt gggaataatc aggtattggt atctggagaa
     8161 tattcgattt ataccggtga tgtaaatcag gatgaggttg tggatgtaac ggatcttgga
     8221 ttgattgata atgatgcttc taattttgtt acgggttatg ttgatactga tcttaattat
     8281 gataatgtag tcgatgttac agatgcatct tttgctgata ataatgctgc cggatttgtt
     8341 tctgtttcca aaccataata attttatatt aattattaaa aatataattt tcagagaatt
     8401 gaaaaatagt atttgaatag ttaactttgg gcaattaaaa agttttttta aaataaaata
     8461 aataaaaatg aaatcattaa atttgttatt agttgttgtt ttatttttaa caactgcgat
     8521 cgtcggagca cagactgatg atgtaaaaat tcaaaccaat tcagatgtaa gttccgaaaa
     8581 gctgactgaa agtattgctg atcagccggg attagacggt ctgatgacag agttagtaaa
     8641 tggaatcaac gtaagtgctt ttaaagatgg tgaaaccggt cagggtgaag tactgaaaat
     8701 gctttccggt cttggtgctg aagatataaa caaatatggt ctggcattag gaagtcttgc
     8761 aggagcatta aacagttccg cttttttacc tacctggacg gaaaatgaaa aaggaattat
     8821 gtctcaatta caaaatgcag tttcaatttc agatgttgca ggcgccgtac ttgaaatgtc
     8881 aggtatgatt gacagatcat cttttacaga aggatttgtt gaaaatatta gctcatggca
     8941 gaactcgtta gaatcaattt ccatgacaaa gtaatttaga atttacgttt tacagttttt
     9001 aatatcccgg ataacttccg ggattttttt atttaatttg tttgaattct gttaatctaa
     9061 attcgaagtt taatttaaaa attttaattt aaaaaattat gaaaaaatta tttaatattt
     9121 tcctgttttt aatatttatt atatcaggta ttgcaggagc acaaactgat aattcaaaag
     9181 gtgatacgaa aaaaaaatca aagagtaatg attttctgaa ttctcagaca ggacttaatt
     9241 ctttgatggg tcagtttgtc aaagggataa ataacagttc attctcagat ggaaagaccg
     9301 gtaagaatga agtgttggga atgcttaaag gcgttaatac ttctgattat ctgaaatatg
     9361 ccggaatagt aggaagtctt gcaggagctt taaaaggtac atctttttta cctgactggg
     9421 caagtaacga aagtggagtt ctggatcagc tgcaaaatgc agcttcaatt gcagatgtag
     9481 caggaggttt actggagatg tccagtatgt tagatccttc ttcttttaca aaaagttttt
     9541 cgaaaaagaa aagctcatgg tcaagtgcac tgaatgtttt atctatgctg aaataaatct
     9601 tacgttttta tagaaataat aaaaatcctg aattttgata ttcaggattt ttttttgatt
     9661 aattccaaaa aataatttat tcgaaaaatc tt
//
LOCUS       DAORVQ010000123         9568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3854_length_9568_cov_4.650899, whole genome shotgun sequence.
ACCESSION   DAORVQ010000123 DAORVQ010000000
VERSION     DAORVQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3854_length_9568_cov_4.650899"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLX80_10010"
     CDS             complement(<1..362)
                     /locus_tag="PLX80_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQY21164.1"
                     /translation="MKINIHNLRETENDLEFNFSKEDLDIENINFNSGIIVNTKVIKT
                     GSQVDIKVKLQGKYEYECDRCLEMMEGKIDNDFELIYKLDLRNSESDFDTDDDIKFIP
                     PNTQTINIKEDIRDFVLIS"
     gene            complement(414..797)
                     /gene="gcvH"
                     /locus_tag="PLX80_10015"
     CDS             complement(414..797)
                     /gene="gcvH"
                     /locus_tag="PLX80_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQY21165.1"
                     /translation="MNFPAALKYTKEHEWLKLDGDVCIIGVTDYAQSELGDIIYLDIT
                     TDVGSEVSAGDVIGSIEAVKTVSEVFSPISGKMLEINSGVNDNPSVVNTDPYDGGWIV
                     KIQPANMDEVSNLLSSEDYKALIGV"
     gene            1279..3600
                     /locus_tag="PLX80_10020"
     CDS             1279..3600
                     /locus_tag="PLX80_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21166.1"
                     /translation="MNNSFAQIPSYSLQLKNLILVDSKTVTFDIEFTHTNSSVWETAA
                     WQYFFRVNSDFFSGGTVTFAYDSLGGDAVSDLPDSFRPRNPQVVVAGNYPGYHELRLA
                     GNNVPGAGNGLIIPQNVPTLIGRMKLKSSNNLDQSFGNFVIRDSCDLPVSITRTKLTT
                     YIGVTNTEVTRCADHSVNLFFAIPPTTLFSSPNQIITMGQSASFIDESYNNPTAWKWQ
                     FPGGIPSSSSSQNPTGIVYNLPGYYDVTLISTNSFGSDTLKKTAFIKSNTLCPFTWQT
                     NIIASDNGNKSDSIKIGMSSVGTNLLDTCLGEYIIPPVPPNGIFDFRSRLSNGEFVKT
                     DFRNDTLKTSTWKLNFQPSTSGYPMTFSWNISEFPSYGFFYLKDSLGTLVNVNMRNQS
                     SYVLTSSALSNLNIIYISDTTKTISVLKGWNLVSVPLLLQNMAVTSIFPNATSPAYSF
                     NNGYLNSPILENGKGYWLKFDTARNYSLDGQFVNLQQMNVTNSWNLIGPFDKDMPVNS
                     IVSNPSGLISSYFFGFNDGYIIADTLRAGKGYWIRSTGNGNLENGSFDNDNVNTVQSF
                     ENMVSLNFSDESGKSIQLYMTNKSGIVDLAQLPPVPPTGVFDVRYSTGNFVESSDKNQ
                     IIQISSIDRPVKFKISNLEGNKFRIKDNIDGSILNMELTENEEITINPALDNFTIINE
                     SQIPQNYELSQNYPNPFNPSTKIMYQLPEEGKVKIIIYNVLGKEVYTLVNSVQQAGRY
                     EVNFNASDLSSGVYFYRLESGNFTDLKRMILLK"
     gene            3623..5398
                     /locus_tag="PLX80_10025"
     CDS             3623..5398
                     /locus_tag="PLX80_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21167.1"
                     /translation="MKVLFTTLCLMFSVCICEAFLSSIYSLEGSMNAHKNKISEFDKQ
                     KIFNSILFQNSNNLFLNIPKSFADNSTDAKEMNNNLNTCSTSWESVLTCVDANNKRDS
                     VKIGKSVMGSIGIDTCLGEYLIPPAPPEGIYDFRIVFPPTYTLDASKTDIRWEGITQA
                     TFWLYFQPSVNGPLTFSWDSTSFPDSSIVLLKEEINGITIDMKKQKQFVINNSGIRYL
                     KVEYKKRKVSYIQVINGWNITSIPLLNYDMSISHLFDSDVSTSFSYNNGYNNANILTN
                     SVGYWIKFPADKNYLINGDILINKEINVITGWNIIGPFEEKIPVSGLVTDPPGLLASP
                     FFGYENGYFSEDTLLPGKGYWIKSNDTGKFYLDTNIIFNRSNPDNKENSFTDWVRIEI
                     SDNSGSVSNLYLAEENEIISDYELPPVPPTGIFDSRFVSNKCVESIEHDNLIQINSVD
                     RPVILKAYNLKGKQLKIQDNIGGSLLNELLSENISVRINNSLDKLLITSESNLPLHYE
                     LSQNYPNPFNPSTTIDYQIPNNGNVKLIIYNILGQEINILLNEFKQSGRYNVEFNAAD
                     LSSGIYFYKIESGSFNEIKSMILLK"
     gene            5486..6511
                     /locus_tag="PLX80_10030"
     CDS             5486..6511
                     /locus_tag="PLX80_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21168.1"
                     /translation="MPLIISDGSALGVDTLFFGIDPLATNGIDAGFGEQELPPPPPSG
                     VFDIRFIDTHLRDSIEFAQGIIQGLEKDYRQGNSLTVTPRTHEVQFKKGSGNVIQFNF
                     PFFPPHTFSLRFVDLFGGILLDTTFTAPGTLTSNYFNINDKVFIYANYQDLTPVELLS
                     FSHSLSGNNVLLKWITSIEINNSGYEVQRKNNSEDQWQVLGFVESNSNSINVQEYTYS
                     DNNLKTGTYSYRLKQIDFNGNFEYLNLNEFVFIGNPNSVQLFQNYPNPFNPNTKISFQ
                     LPATGNLELSIFDTKGMLIENILNGEFNEGYYNINFSAENLASGIYFYRLNFNGNSVS
                     KKMIVSK"
     gene            complement(6556..7245)
                     /gene="ubiE"
                     /locus_tag="PLX80_10035"
     CDS             complement(6556..7245)
                     /gene="ubiE"
                     /locus_tag="PLX80_10035"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQY21169.1"
                     /translation="MQPDKSRKKIEEMFDEIAPTYDKLNHLFTARSDIIWRRKIVKYL
                     ISTGRNFYNIVDLASGTGDLTIELLKLEPKKIYAVDISKKMLEIQKEKVNDKRLELIQ
                     AEAANMPFDDNSIDLVTIGFGIRNFEDLEISLKEIKRVLKNDGYLIVLEMFKAEKISS
                     KLFNYYFSRIMPFLGNKLSKSKTAYNYLSDSVQNFLTVKEFSDICKNNGFETEKTVNN
                     FIGVVNTIYLK"
     gene            complement(7251..8471)
                     /locus_tag="PLX80_10040"
     CDS             complement(7251..8471)
                     /locus_tag="PLX80_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21170.1"
                     /translation="MKNYKLLIARRFLFSKGESRFISFITYISILGVTLGVSALIIAV
                     SVLNGFEKEIRDKVAGLVSHIQISSFVTEGLSNHEEAIAKMKNEIEGIKGISPIVQKE
                     AVIRYKSTVEGIILKGINIETDLSTAKSKVVSGTFDLTPIDSSFSKLIIGDKLAKKLG
                     IETGNKVIVFGLNGVPGPMNQPKIKQFIVSGVYETGLKDLDDIIIYTDIKTTQNLFDF
                     GNNITGIEIELNDVNKAEDVVKIIRTDIGYPYFPKSLFNLYKPLFTWVELQKAPIPIV
                     LGLIILVATFNIVGTLLMLVLEKTQSIGVLKTLGVSKSGIVKIFLFDGVLIGIIGIIL
                     GNLIGLGLCYLEMKFNFFALPDIYYMKSVPILLNPDYIIFISIITFILTVTATLIPSF
                     LASRLDPVKSIRFS"
     gene            complement(8473..9565)
                     /gene="prfB"
                     /locus_tag="PLX80_10045"
     CDS             complement(join(8473..9504,9506..9565))
                     /gene="prfB"
                     /locus_tag="PLX80_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_144079050.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQY21171.1"
                     /translation="MFEQQKYRLKDSKEKIDSLRGFFEIDNKISKIKELDSQTTVENF
                     WEDNKAAQKILQEISALKKWVEEFQGVESKFIDINGLIEIAESEKDETLTDEIEKDID
                     IFEKELDEVEFKNMLSDKDDVRDAIITINSGAGGTESQDWAEMLLRMYLRYCEKNGLN
                     ALLIDRLDGDGAGIKSATVEVKGEFAYGYLKAENGVHRLVRISPFDSNKKRHTSFAAV
                     YVSPVIDDEIEIELNSADMKIDTFRAGGAGGQNVNKVETAIRITHAPSGIVVQCQNER
                     SQNQNKMNAIKMLKSKLYMLEKEKELARLQTIENTKKKIEWGSQIRSYVFHPYNMVKD
                     HRTDHETSDTQGVMDGDIHDFIKAFLLEK"
BASE COUNT         3148 a         1601 c         1391 g         3428 t
ORIGIN      
        1 gaaattagaa caaaatcacg aatatcttct tttatattaa ttgtctgtgt attcggtgga
       61 atgaatttta tatcatcatc tgtatcaaaa tcactttccg aatttcttag atctaattta
      121 taaattaatt caaaatcatt gtcaattttt ccttccatca tctcaagaca tctgtcacat
      181 tcatattcat atttaccctg gagttttacc ttaatgtcaa cctgacttcc ggttttaatg
      241 acctttgtat ttactataat acctgaattg aaattaatat tttcaatatc cagatcttct
      301 tttgaaaaat taaattcaag atcattttcg gtctctctta aattgtgaat attgattttc
      361 atttctaatc ttatattaaa gaacaattaa tgtttggttc cctacagatc tttttatact
      421 ccgatcaaag ctttgtaatc ctctgagctg agaagattgc ttacttcatc catattagcc
      481 ggctgaattt tcacaatcca tcctccgtca taaggatctg tatttactac tgatggatta
      541 tcattaacac ctgaatttat ttcaagcatt tttcctgata taggtgaaaa cacttctgat
      601 acagtcttaa cagcttcaat agaaccaatt acatcacctg cactaacttc acttcctaca
      661 tctgttgtaa tatctaaata tataatatca ccaagttcac tttgtgcata atcagtaact
      721 ccaattatac acacatcacc gtcaagcttt aaccactcgt gctctttagt atatttaagg
      781 gcagcaggaa aattcatctg aaataaaatt tgatttaaga aaatgcaatt taattatttt
      841 aaatctgtga ttcaattgag aaaatagact tattgattta ttttaattat tctgatatta
      901 tattatatta cttaaatatt tattctctat atccaattaa aatattgaaa acaggaatga
      961 cttttttatc tgaactttaa tttaattttc agctattctc atcttattta taaaatgata
     1021 ttccttatgt aattttttta attattatgg tacaaatgta atttacgtaa attaagaatt
     1081 tgtgataaat taaaatgctg ttattttaat atagagtatt tatctttaat agattaaaaa
     1141 aaaaatttta ttgtttgcaa tactgtttta atattttcat tatgtaatct taagtaaatt
     1201 ttaatttaaa aaaatcttaa acacaatcag tttatgaaaa agttttacct gtctctttgt
     1261 ttattattat tcttctatat gaataatagc tttgcacaaa ttccatcata ctctcttcag
     1321 ctaaagaatt taattttagt tgattcaaaa acagttacat ttgatattga atttactcat
     1381 acaaactcta gtgtttggga gactgctgca tggcagtatt tcttcagagt aaacagtgat
     1441 ttcttttcag gtggtacagt cacttttgca tatgattccc ttggtggaga tgcagtctca
     1501 gatcttccgg attctttcag gcctagaaat ccacaggtag tagttgctgg aaattatcca
     1561 ggctatcatg agttgagact agcaggaaat aatgttccag gagcagggaa cggacttatc
     1621 attcctcaaa atgtacctac actaatcgga agaatgaaat taaaaagttc gaataatctt
     1681 gatcagagtt ttggaaactt tgttattaga gatagctgtg atttgcctgt ttcaattacc
     1741 agaactaaat taactactta tattggagtt acaaatacag aagtgacaag atgtgcagat
     1801 cattctgtta atttgttttt tgcaatacct ccgacaactt tatttagttc tcccaatcaa
     1861 ataatcacaa tgggtcaaag tgcatctttt attgatgaga gttataataa tcctacagca
     1921 tggaaatggc aattccccgg aggaatccca tcaagctctt catcccagaa tccaacaggc
     1981 attgtttata atttacccgg ttattatgat gttactttaa taagtacaaa ttcatttggt
     2041 tctgatacat tgaaaaaaac tgcattcata aaatcaaata ctctttgtcc gtttacatgg
     2101 cagacaaata taattgcatc tgacaacggc aataaatctg atagtataaa aattggaatg
     2161 tcatctgtcg gcactaacct tttggataca tgtctcggtg aatatattat tccgccagtt
     2221 cctccaaacg gaatttttga ctttagatca agactttcaa atggtgaatt tgtaaagaca
     2281 gacttcagaa atgatactct taaaaccagt acctggaaat taaattttca gccttcgact
     2341 tctggatatc ctatgacatt ttcatggaat atttctgaat ttccttcata tggtttcttc
     2401 tatttaaaag attctcttgg aactttggtt aatgtaaata tgagaaatca aagcagctat
     2461 gttttaactt catcagctct gagtaattta aatattatat atatttctga cacgaccaaa
     2521 actatttcag tgttaaaagg atggaactta gtaagtgtac cattactttt acagaacatg
     2581 gctgtaacaa gtatatttcc aaatgctacg tcacctgcat attcatttaa taatgggtat
     2641 ttaaattctc caattcttga aaacggaaag ggatactggc tgaagtttga tacagccaga
     2701 aattattctc tcgatggtca gtttgtaaat cttcagcaaa tgaatgttac aaattcatgg
     2761 aatctcattg gaccatttga taaagacatg ccggtaaaca gcatagtgtc aaatccttct
     2821 ggtctaattt cctcttactt ctttggattt aatgacggat atataatagc tgacactttg
     2881 agagcaggta aaggttactg gataagatca actggaaacg gaaatctgga aaatggttct
     2941 tttgataacg ataatgtgaa cactgttcag tcttttgaaa atatggtatc acttaatttt
     3001 tctgatgaat caggaaaatc cattcagttg tatatgacaa ataaaagcgg aattgttgac
     3061 cttgcacagc ttccacctgt acctccgaca ggagtatttg atgtaaggta tagcacagga
     3121 aattttgttg aatcttcaga taaaaatcaa ataattcaga tcagctcaat tgacagaccg
     3181 gttaaattta aaatttcaaa tctggaagga aataaattca gaattaaaga taatatagac
     3241 ggcagcattt tgaatatgga gctgacagaa aatgaagaaa ttacaatcaa tcctgcttta
     3301 gataatttta caataataaa cgaatctcag atacctcaaa attatgagct gagtcagaat
     3361 tatcctaatc catttaatcc ttctacaaag ataatgtatc aactgccgga agaaggtaaa
     3421 gtaaaaatta ttatctataa tgttttggga aaagaagtgt atactcttgt aaattcagtt
     3481 cagcaggctg gaagatatga agttaatttt aatgcttctg atctttcttc aggtgtatat
     3541 ttttacagat tagaatcagg gaattttaca gatctaaaaa gaatgatctt attaaaatag
     3601 tttttattta aaatattaat ttatgaaagt acttttcacg acattatgtt taatgttttc
     3661 agtttgcatt tgtgaggctt ttttatcttc tatttattca ttagaaggat ctatgaatgc
     3721 acacaaaaat aaaatttcgg agtttgataa acaaaaaatt tttaattcta tattatttca
     3781 aaattcaaat aatttatttt taaatattcc aaagtcgttt gcagataaca gtaccgatgc
     3841 aaaagaaatg aataacaatt taaatacatg ctccacttcc tgggaatctg ttttaacatg
     3901 tgttgacgca aataataaac gggattcagt caagattgga aaatcggtaa tgggaagtat
     3961 aggtattgat acatgtctcg gagagtattt gattccacct gcaccaccgg aagggattta
     4021 tgattttaga atagtgtttc ctcctactta tactcttgac gcatctaaaa cagatatcag
     4081 atgggaagga ataacacagg caactttttg gttgtatttt caaccatcag ttaatggacc
     4141 acttacattt tcatgggatt caacttcatt tcctgattca agtatcgtac ttctgaagga
     4201 agaaattaat ggaattacta ttgatatgaa aaagcaaaaa caatttgtaa taaataattc
     4261 cggaatcaga tatttaaaag ttgaatataa aaaaagaaaa gtatcctata ttcaggtaat
     4321 taacggttgg aatattactt ctataccttt attgaattat gatatgtcta tttctcattt
     4381 atttgattca gatgtctcaa cctcgttcag ttataataat ggttataata atgctaatat
     4441 tttaacaaat agtgttggat actggatcaa atttcctgct gataaaaatt atttaatcaa
     4501 tggtgacatt ttaattaaca aagaaattaa tgtaattaca ggatggaata ttatcggacc
     4561 gtttgaagag aaaattccag tgagtggact ggtaactgat cctccgggtc tgttggcatc
     4621 ccctttcttt ggctacgaaa atggatattt ttctgaagat acacttcttc ccggaaaagg
     4681 ttactggatc aaatctaatg acaccggcaa gttttattta gatacaaata ttatttttaa
     4741 cagatcaaat ccggacaata aagaaaattc ttttactgat tgggtgagaa ttgagatctc
     4801 agataattcc ggaagtgttt ccaatcttta tcttgctgaa gaaaatgaga tcatttcaga
     4861 ttacgaactt ccacccgttc cgccaacagg aattttcgat tcacgatttg tttcaaataa
     4921 gtgtgttgaa tctattgaac atgataatct gattcaaata aattctgttg acagaccggt
     4981 cattttaaaa gcatataatc ttaaaggtaa acagctcaaa atacaagaca atattggcgg
     5041 ttcgttgtta aatgaattat taagtgaaaa tatttctgtc agaataaata attctttaga
     5101 taaacttttg attacatcag aatcaaattt acctttgcat tatgagcttt cacaaaatta
     5161 tcctaatcct tttaatcctt ccactacaat agattatcag attccaaata atgggaatgt
     5221 aaaacttata atttataata ttttgggaca ggaaattaat atacttttaa atgagttcaa
     5281 acagtccggt agatataatg ttgaattcaa tgctgcagat ctttcttccg gaatttattt
     5341 ctacaagatc gaatccggta gttttaatga aataaaatca atgatacttt taaaataatt
     5401 ttaataagaa attatgaaaa aaatatttat cactttaatc ttttgctttt ttataagcaa
     5461 tatttccatt tcccaatttg taaaaatgcc gttaataatt tcagacggtt ctgctttagg
     5521 tgtcgatact ttatttttcg gtattgatcc tttagcaact aacgggattg atgccggttt
     5581 tggtgagcag gaattaccac ctcctccccc ttccggtgtt tttgatataa gatttataga
     5641 tactcacctt agagacagca tagaattcgc tcagggaata attcaaggtc ttgagaaaga
     5701 ttacagacag ggaaacagtt taactgtaac tccaagaaca catgaagtac agtttaaaaa
     5761 aggatccgga aatgtcattc agtttaattt tccgtttttt cctcctcata ctttttcatt
     5821 aaggtttgtt gacctttttg gcggaattct tttagatact acttttactg ctccaggtac
     5881 tttgacaagc aattatttca acattaatga taaggtcttt atatatgcta attatcagga
     5941 tcttacaccg gtagaattgt tatcattttc tcattccctg tcaggtaata atgttctttt
     6001 aaaatggatc acttctattg aaataaataa ttcaggatat gaagtgcaga ggaaaaataa
     6061 ttcagaagat caatggcagg tacttggttt tgtcgaatct aacagcaatt ctataaacgt
     6121 acaggaatat acatattcag ataataattt gaaaaccggc acttattcct acagattgaa
     6181 acagattgat tttaatggta actttgaata tctgaatttg aatgaattcg tttttatcgg
     6241 aaatccaaat tcagttcaat tgtttcaaaa ttatccaaat ccatttaatc caaatactaa
     6301 aatatcattt cagttaccgg ctaccggtaa tttagaatta tctatatttg atacaaaggg
     6361 tatgctgatt gaaaatattt tgaatggtga atttaatgaa ggatattaca atataaattt
     6421 tagtgctgaa aatctagcaa gcggaattta tttttacaga ttaaatttca acggaaattc
     6481 agttagtaaa aaaatgatag tatctaaata atccggactg aataattttc tataagaaat
     6541 ttcatatatt tatttctact tcagataaat tgtgtttact actccaataa aattgttcac
     6601 agttttttca gtttcaaaac cattattttt acagatatct gaaaactctt ttactgtcag
     6661 aaaattctgt acagaatctg atagataatt ataagctgtc ttactttttg ataatttatt
     6721 tccaagaaaa ggcataatcc tgctgaagta ataattaaaa agttttgaac taattttctc
     6781 agctttgaac atttctaaaa caatcagata accatcattt tttaaaactc tctttatttc
     6841 ttttaatgaa atttcaagat cctcaaaatt tctgatacca aatcctatcg taaccagatc
     6901 aatactgttg tcatcaaatg gcatgtttgc tgcttctgcc tgaattagtt ccagtctttt
     6961 atcattaact ttttcttttt gaatttcaag catctttttt gaaatatcta cagcgtatat
     7021 ttttttaggt tcaagcttta aaagttcaat ggtcagatca cccgtacctg aagcaaggtc
     7081 gacaatattg taaaaatttc taccggtaga tattaaatat ttcacaatct ttcgcctcca
     7141 gataatatca cttctcgcag taaataaatg atttaactta tcataagtcg gagcaatctc
     7201 atcaaacatc tcctcgatct tttttcttga tttgtcaggc tgcattctta ttaactaaat
     7261 ctgattgatt taacaggatc taacctggaa gcgagaaagg aaggaattaa tgtagctgta
     7321 acagtaagta tgaatgttat tatcgatata aaaattatat aatcgggatt caataatatc
     7381 ggtacacttt tcatataata gatatcagga agtgcaaaga aattaaattt catttctaaa
     7441 taacaaagac caagaccgat cagatttcca agtatgatcc cgattatacc aatgagcact
     7501 ccgtcaaata aaaatatttt aacgattcca gatttactta cgcccagtgt ttttaaaact
     7561 ccgattgatt gtgtcttctc aagtacaagc atcaaaagcg ttccgacaat gttaaaagta
     7621 gctacaagta ttatcagtcc aagaactata ggtatcggtg ctttctgaag ctctacccat
     7681 gtaaatagtg gtttgtataa attgaaaagt gactttggaa aatatggata tccaatatcg
     7741 gttcttatta tcttaactac atcctcagct ttatttacat cattcagctc gatctcgatt
     7801 cctgtaatat tatttccaaa atcaaaaaga ttctgtgtag tttttatatc agtatatata
     7861 attatatcat caagatcttt cagaccggtc tcatatactc cgcttactat aaactgtttt
     7921 atctttggct gattcattgg acctggaact ccgttcagac cgaatactat gactttgttc
     7981 cctgtttcta taccaagttt ctttgccagc ttgtcgccta tgatcagttt tgaaaatgaa
     8041 ctatcgatcg gagtcagatc aaacgttcca ctgacaactt tactttttgc agttgacaga
     8101 tcggtttcaa tgttaattcc ttttaagata attccttcca cagttgattt atatctgatc
     8161 acagcttcct tctgaacgat cggagatatt ccttttatac cctctatctc atttttcatc
     8221 ttagcaattg cttcttcatg gttacttaat ccttcagtta caaatgatga aatttgaata
     8281 tgtgaaacaa gaccggctac tttatcgcgt atctcttttt caaatccatt aagtacggat
     8341 actgcaatta tcaaagctga cactccaagt gtaactccga gtatagaaat ataagttata
     8401 aaagaaataa atctggattc gcctttagag aatagaaacc gtcttgcaat taaaagttta
     8461 taattcttca atctatttct caagtaaaaa tgcttttata aaatcatgga tatctccgtc
     8521 catgactccc tgtgtgtcgc ttgtttcatg atctgtcctg tgatctttga ccatattgta
     8581 aggatggaaa acgtaacttc ttatctgact cccccactca atttttttct ttgtgttttc
     8641 aattgtctgc aatctcgcaa gttctttttc cttttcaagc atataaagtt ttgacttcag
     8701 catctttatt gcattcattt tattctgatt ctgtgaacgc tcattctgac attgtaccac
     8761 aattcctgaa ggagcatgtg taattcgaat tgcagtttct actttattta cattctgacc
     8821 gcctgctcct cctgcacgaa atgtatcgat cttcatgtct gcagaattta attcaatttc
     8881 aatctcatca tcaattacag gactaacata aacagcggca aacgaagtgt gacgtttttt
     8941 atttgaatca aaaggagaga tccttacaag tctgtgaaca ccattctcag ctttaagata
     9001 gccataagca aattctcctt tgacctcgac agtagcgctt tttattcccg ctccgtcacc
     9061 atctaatctg tctatcagca atgcattgag accattcttc tcgcaatatc tcagatacat
     9121 tctcaaaagc atttcagccc agtcctgaga ttcagtcccg cctgcacctg aattgatcgt
     9181 aattatagca tccctcacat catctttatc agaaagcata tttttaaact caacttcatc
     9241 aagttctttt tcaaaaatat caatatcttt ttcaatctca tctgttaaag tttcatcctt
     9301 ttccgattca gcaatttcaa tcaatccgtt aatatcaata aattttgatt caactccctg
     9361 aaattcttct acccattttt tcagagcgga tatttcctga aggatctttt gtgcggcttt
     9421 gttgtcttcc caaaaattct caactgtagt ctgagaatcc aattctttta ttttagagat
     9481 cttgttatcg atttcaaaga aacctccgta atgaatctat tttttctttc gaatctttta
     9541 atctgtactt ttgctgttca aacatttt
//
LOCUS       DAORVQ010000124         9544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3869_length_9544_cov_4.354621, whole genome shotgun sequence.
ACCESSION   DAORVQ010000124 DAORVQ010000000
VERSION     DAORVQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3869_length_9544_cov_4.354621"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLX80_10050"
     CDS             <1..250
                     /locus_tag="PLX80_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21172.1"
                     /translation="FGTLFSFFYNALDYMDYPMTRKLTGFRKKLGIILSQRKSSMGFG
                     AMAFILTFIPVINVLINPLLVVSGTSLFYKNKYNISDQ"
     gene            247..798
                     /locus_tag="PLX80_10055"
     CDS             247..798
                     /locus_tag="PLX80_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21173.1"
                     /translation="MIIISAHADTNYKRVSLIIEGEDYVGFLDNYVGVYAAMKAFFSG
                     QINFDYVRLELTPDEEIDMRGAREVAKEVSKEDLVIVIDVTGTPTDKDFVIEKCASEK
                     VSSFLNDVLSGFSYDIYKDCPDPVANMDETDVYRHKTEHCFFLGLPCSGGDYNFTETR
                     CPVRSVDEAARAVIEICRNFKKF"
     gene            898..3183
                     /gene="purL"
                     /locus_tag="PLX80_10060"
     CDS             898..3183
                     /gene="purL"
                     /locus_tag="PLX80_10060"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQY21174.1"
                     /translation="MSDPEINLDVAKSLGLLEDEYERILKILGRIPSYTELGIFSVMW
                     SEHCSYKNSILEIKKLPRSGKRLLVSAGDENAGLVDIGDGLAVAFKIESHNHPSAVEP
                     YQGAATGVGGILRDIFTMGARPVAALNSLRFGKIDFGNNSNNSNNSSDEQRKRNKFLF
                     KNVVKGIGDYGNCFGVPTISGEVYFEDCYNDNPLVNAMAVGIVKHDGTATAEAGGIGN
                     PVFIVGSSTGKDGIHGATFASEEISEDSESKRPNVQVGDPFTEKLLLEATLELIKSGA
                     VVGIQDMGAAGITCSTSEMSAKGKCGMEINLDLVPLRDNDMSSYEIMLSESQERMLVV
                     IEKGKEKIAEDIFNKWDLNCVEIGNVIKEDRIKIYYKGKLEADVPAFELVLGGGAPVN
                     VREISFPKYIEEKRKFDPASINEPTDLNQIVLKLLSSPNITNKNWVYEQYDTQVRTNT
                     VVLPGSDASVIRIKETEKALSVKVDCNGRYVYLNPFKGGMIAVCESARNVVCTGAKPI
                     AITNCLNFGNPYNPEVYYQFSEALKGIGDACKILDTPVTGGNVSFYNQSKDHAVYPTP
                     VIGMLGLIENKNNIMTSHFKNEGDIVILISGIDLNIINEKSIGSSEYFRLLKNEPEGD
                     APDINPVVEKEVQEIILKLIESSYIKSAHDISDGGLAIALAECCIMDRNNSIGCEIIY
                     NYTLRKDFELFSEFQSTFIVSADAVNFEKISEMALQKNLKAEKIGIVSGKNLKINNDI
                     DLHLEELNESYFNSLNTLMKE"
     gene            3373..3996
                     /locus_tag="PLX80_10065"
     CDS             3373..3996
                     /locus_tag="PLX80_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21175.1"
                     /translation="MSSDLTASEFSLSKKRSVLNDPTDHFLFSQYKSSLSDRNESSAS
                     TLPIAIGVASFLYLFNPILLLENDKIGGGITKEVSLGFGKFGQHRFSTEYSYIFRQDL
                     SSNLRFAYKYDILLKSGLKPSNFLQGTTVLSIGGGYFTNFTNHGYFPELSYGYSIRND
                     KLLFFPSLKIRYTYVVNGADIVDFSFGLVIGIANPFIDLNIRANDSK"
     gene            4046..4852
                     /locus_tag="PLX80_10070"
     CDS             4046..4852
                     /locus_tag="PLX80_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21176.1"
                     /translation="MAEVTSDKSKPLFNIKLPDSAQETFFKITSLVFFAFRNIKEIFM
                     PPYEFSETLKQCYQIGYKSLSLIAITGFIIGLTLTMQSIPTLQKFGASSLVPSMVALA
                     VILEIGPVITALIFAGKIGSGIGAELGSMKVTEQIDAMEVSGCNPFKYLVVTRVTAAT
                     LMLPILVILADMIALIGGFVAFSISENSSMKAFLISAMSNLSLQDIILSILKTFFFGF
                     SIGVVGCYEGYNADRGTESVGVAANTAVVISSLLVILIDMLMVQITSIIF"
     gene            4911..5669
                     /locus_tag="PLX80_10075"
     CDS             4911..5669
                     /locus_tag="PLX80_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY21177.1"
                     /translation="MSEDPKTNTNEIVLEITDLKKSFGDNTVLKDINIEIKKGESVVM
                     LGKSGTGKSVILQCIIGLLPPTKGTIKVFDTDFLKLPEVEKNSIRRKIGFLFQSGALY
                     DSMTVRQNLEFPIIRQPDYKKEDLSEKITEILESVSLSEAIDKMPSELSGGMKKRIGL
                     ARTLMLDPEIILYDEPTTGLDPVTSNEISHLILDMQKKLNVTSLIVTHDMACAKLVAD
                     RIIVLKDGTIAAEGSFDELAKSDDEFVRGFFEFE"
     gene            5803..6885
                     /locus_tag="PLX80_10080"
     CDS             5803..6885
                     /locus_tag="PLX80_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQY21178.1"
                     /translation="MGINKSKYIKLGIFIITGLFLFVFALFYIGSKENLFTNTFNVYS
                     VFSNVSGLTQGNSVQYAGINVGTVESIEILSVDQVRVNMNLVSDVQKFIKKDSKATIS
                     SDGLVGSKIVSITPGKFESVMVENGDSIHSVNPVSMGDIINNLNESSKEAEVIAKELT
                     EIMSKINNGEGTLGKLVNNSSMYDNLDSLLNSFAGSTAQINNILTQTSNTIDMVAGDI
                     QEMQGSVKLIVTNIGDITSKINSSQSLVGTLLTDTVFANNIKGVVKNANFTTQNLEMG
                     SFSFYQNMEALKHNFLFKGYFEDLGYWDQSKEEKDYISREERILNKEQELNIKELQLR
                     EFQEKLDSLKNNLQIKIDTSKTGESK"
     gene            6982..7230
                     /locus_tag="PLX80_10085"
     CDS             6982..7230
                     /locus_tag="PLX80_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQY21179.1"
                     /translation="MTNAEIEAKVVSAVVDKLGVEESKVTTDASFINDLGADSLDTVE
                     LVMKFEEEFDIKIPDEDAEKIQKVGDAVSYITEKISAN"
     gene            7265..8512
                     /gene="fabF"
                     /locus_tag="PLX80_10090"
     CDS             7265..8512
                     /gene="fabF"
                     /locus_tag="PLX80_10090"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQY21180.1"
                     /translation="MKRRVVVTGIGVVSPVGLNINDFWSSLIAGKSGVDNITYFDATD
                     FDTKFAAELKGFDPLNYMDRKLSQRVDPFTQYALVASEEAIVDSGLNLEKIDSDRAGV
                     VYGSGIGGMWTYHNQQTNLYKRDGNPDRISPFFIPMLIADIAAGRISMKYGFKGPNYA
                     TISACTTSAHSIADSVMLIERGDADIMITGGSEAVICPMGVGGFNSMKALSTRNDAPQ
                     KASRPFEKNRDGFVMGEGGATLILEELEHAKSRGANIYAEITGFGLTADAFHITEPAP
                     EGEGVGRAISIAIKNSNLTINDIDVINAHGTSTYYNDKNESMAIRNVFKEKADQIPVH
                     SIKSMIGHLLGAAGAIEAISSILTIRDGIIPPIINYEEKDPECDLNYVVNTAMKKDVK
                     TVLSENSGFGGHNTAILFKKYEE"
     gene            8538..9362
                     /gene="rnc"
                     /locus_tag="PLX80_10095"
     CDS             8538..9362
                     /gene="rnc"
                     /locus_tag="PLX80_10095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQY21181.1"
                     /translation="MFNLLKNRLRKLTPFFETRKEKKNLSKKLTDFIDFDELQNNIHY
                     KIIDENYFVNALTHRSFLTVRSDLSQVGIISNERLEFLGDAVLDLVVAEYLYKNFPLN
                     EEGDLTKFRSILVNKKFLAERAKHLKLQDFLLASYTAKKSIDEGYDAILADTYEALIG
                     AVFMDSGYENAKEFLNEEIFIKLDIKWLNQFDENHKSKFLEYVQAHTDFIPEYSIIKE
                     EGPEHNKLFTVEVFVNKRSLGIGKGKSKKQAEQASAKNALNNLEVLEKMGLTKKKS"
BASE COUNT         3399 a         1350 c         1789 g         3006 t
ORIGIN      
        1 attcggaact ctattctcat ttttttacaa tgcgcttgat tacatggact atcctatgac
       61 aagaaagctg actggattca gaaaaaaact tggcattatt ttatctcaaa gaaaatcatc
      121 tatggggttt ggagcaatgg cattcatact tactttcatt cctgtgataa atgttcttat
      181 caatcccctt ctggtagttt caggtacttc acttttttat aaaaataaat ataacatttc
      241 agatcaatga taattatatc tgcacatgcc gatacaaatt acaaaagagt aagtttaata
      301 attgaaggtg aagattacgt tggtttcctt gataattatg tgggagttta tgctgcaatg
      361 aaagcttttt ttagtggaca gataaatttc gattatgtca gattggagct tactcctgat
      421 gaagaaattg atatgagagg cgcacgtgaa gttgcgaagg aagtcagtaa agaggatctc
      481 gttattgtaa ttgatgtaac cggtactccg actgataaag attttgtcat tgagaaatgt
      541 gcatcagaaa aagtgagttc atttttaaat gatgtactat caggatttag ttatgatatt
      601 tacaaagatt gccctgaccc tgtagctaat atggatgaga ctgatgtata cagacataaa
      661 actgagcact gtttttttct cggacttccc tgcagcggag gtgattacaa ttttactgag
      721 acacgatgtc cggtcagatc tgtagatgaa gctgcgagag ctgtgataga gatctgcaga
      781 aattttaaaa aattctgatc tgctttttaa attccaaaaa atactcatta aataatttta
      841 tctgactaat aaaactattg ccatttaact atacttgtta aaattttttt acaaaaaatg
      901 tctgacccgg aaataaattt agatgttgct aaaagtcttg gattattaga agatgaatat
      961 gaaaggatct taaagatcct tggacgtatt ccttcataca cagaacttgg tattttttca
     1021 gtaatgtgga gcgagcactg ctcttacaaa aattccattc ttgaaattaa aaaacttcca
     1081 cgcagcggta aaaggcttct agtaagtgca ggtgatgaga atgcaggttt agttgatatt
     1141 ggtgacggac ttgctgtagc ttttaaaatt gaatcacata atcatccttc tgcagtcgag
     1201 ccgtatcagg gggcagctac aggagtcggc ggaattctcc gtgatatttt cacaatggga
     1261 gcaagaccag tcgcagcttt aaattcactg agatttggaa aaattgattt tggaaataat
     1321 tctaataatt ctaacaattc ttcagatgaa cagaggaaaa gaaataaatt cctttttaaa
     1381 aatgtagtaa aaggtattgg agattatggc aactgtttcg gcgtacctac tatcagcggt
     1441 gaagtatatt ttgaagactg ctacaatgac aacccacttg taaatgcaat ggcagtagga
     1501 attgtgaaac atgacggtac tgcaactgct gaagcaggcg gcattggaaa tccggttttt
     1561 atagtaggat catctactgg aaaagacggc attcacggag cgacttttgc atctgaagaa
     1621 atttcagaag actctgaatc caaaagacca aatgtacagg tcggcgatcc gttcactgaa
     1681 aaacttttac tggaagctac attggaacta ataaaaagcg gtgctgtagt aggcattcag
     1741 gatatgggtg cagcaggaat tacctgttcg acaagtgaga tgagtgctaa agggaaatgc
     1801 ggaatggaaa ttaatcttga ccttgtacct ctcagagata atgacatgtc ttcttatgaa
     1861 ataatgcttt ccgaatctca ggaaagaatg cttgtcgtca ttgaaaaagg taaggaaaaa
     1921 attgctgaag atatttttaa taaatgggat ctgaattgtg ttgagattgg caatgtaata
     1981 aaagaagaca gaataaaaat ttattacaaa ggtaaacttg aagcagatgt accggcattt
     2041 gaattagttc tgggcggcgg agctcctgta aatgtaagag agatcagctt tccaaaatat
     2101 attgaggaga aaagaaaatt cgatccggca agtattaatg aaccgaccga tttgaatcaa
     2161 atagttttga agcttttgtc ttcgccaaat atcacaaata aaaactgggt gtatgagcaa
     2221 tatgatactc aggtcagaac aaatacagtc gttctaccgg gcagtgatgc ttcggtgatt
     2281 agaattaaag aaaccgaaaa ggctctttcc gtcaaagtcg attgcaacgg tcggtatgta
     2341 tatctgaatc cttttaaagg aggaatgata gctgtctgtg aaagcgcgag gaatgttgtc
     2401 tgcactggtg caaaaccaat tgcaattaca aactgtctga attttggaaa tccatataat
     2461 cctgaagttt attatcaatt ctctgaagct ctgaaaggaa tcggtgatgc ctgtaaaata
     2521 cttgacacgc ctgtaaccgg cggcaatgta agcttttata atcagtcaaa agatcatgcc
     2581 gtctacccta ctccggtgat tggaatgctt ggcctgatag aaaataaaaa taatattatg
     2641 acaagtcatt ttaaaaatga aggtgatatt gttatattga taagcggaat tgatctgaat
     2701 attataaatg aaaaaagtat aggcagcagt gaatatttca ggcttttaaa aaatgaacct
     2761 gaaggtgatg caccggatat taatccagtg gtagagaaag aagttcagga aataatactt
     2821 aaacttattg aatctagtta tattaaatct gctcacgata tttctgacgg tggacttgca
     2881 attgctcttg cagaatgttg tataatggat agaaataatt ctataggatg tgaaattatt
     2941 tacaattata ctttaagaaa agatttcgaa ttattttctg aattccaaag cactttcatc
     3001 gtttcagcag atgcggttaa ttttgaaaaa atttcagaaa tggctttgca gaaaaatctt
     3061 aaagcagaaa agattggtat tgtatcgggt aagaatttaa agatcaacaa tgacatagat
     3121 ttacatttgg aagagttaaa tgagtcatat tttaattctc tcaatactct tatgaaagaa
     3181 tgatattatt tattttgaaa gtataaattg attttaaaga attgaaaata aaattttaaa
     3241 actgatattc atttgttaaa aaaatcaaaa aaatttaata agaaaaaaag aatattagta
     3301 tttctgatat taatattaat gtctgtaaat tctgaaaaag tattctccca atttatttca
     3361 taccctgata aaatgagttc agatctaact gcatcagaat tttcattatc aaaaaagaga
     3421 tcagttttaa atgacccaac cgatcatttt ttgttttcac aatataaatc ctctttatca
     3481 gacaggaatg aaagttcagc atctacttta ccaattgcta taggtgtagc atcattctta
     3541 tatttattca atccgatact tttacttgag aatgacaaaa ttggcggagg gattacgaaa
     3601 gaggtttctc ttggatttgg aaaatttgga cagcatcggt tttcaacgga atattcatat
     3661 attttcagac aagacttaag cagtaattta cgttttgcat ataagtatga catactttta
     3721 aaaagcggat taaaaccttc aaatttttta cagggaacaa ctgtgctctc tataggtgga
     3781 ggttatttta caaactttac aaatcatggt tatttccctg aattatcata tggatattct
     3841 ataagaaatg ataaactatt attttttccg agtttaaaga taagatatac atatgttgta
     3901 aatggtgcag atattgttga tttttcattc ggtctggtga ttggaattgc aaatccattt
     3961 attgatctta acattcgcgc aaatgatagt aaatagaact tttacggggt tacagatttt
     4021 aatgcaaata aataatttat tttaaatggc tgaagttaca tctgataaat caaaaccttt
     4081 attcaatata aaactaccgg attcggctca ggaaacattt ttcaaaatta caagtctggt
     4141 attttttgca ttcagaaata ttaaagaaat ttttatgcct ccgtatgaat tttcggagac
     4201 cttaaaacaa tgttatcaga tcggatataa atccttatca cttattgcaa tcacaggatt
     4261 catcataggt ctgacattga caatgcaatc tatacctact ctgcaaaaat tcggagcttc
     4321 atcacttgta ccgtctatgg tagcacttgc agttatatta gagataggtc ctgtaattac
     4381 agctttgata tttgcaggta aaattggttc aggaattggt gctgagcttg gctcaatgaa
     4441 agtaactgag cagatcgatg ctatggaagt atccggctgc aatcctttta aatatttagt
     4501 agtcacaaga gttacagcag ctactttaat gcttccgata ctagtaatac ttgctgacat
     4561 gatagcatta ataggcggtt tcgttgcatt cagtatttcg gagaattctt ccatgaaagc
     4621 atttttaata tccgctatgt caaacttaag tctgcaggat ataatattat ccattctaaa
     4681 aacttttttt ttcggattta gtatcggtgt ggtcggatgc tatgaagggt ataatgctga
     4741 cagaggaact gaaagtgtcg gagtggcagc aaatactgca gttgtgattt catctttact
     4801 tgtgatttta atagacatgt tgatggtaca aattacatca ataatatttt aaaaataaat
     4861 aatgcaaatt ttcatcaaat caatttcaaa tttagtcaga taaaagattt atgtcagaag
     4921 atccaaaaac aaataccaat gaaatagttt tagagattac agatctaaaa aaatcctttg
     4981 gggataatac tgttctaaaa gacataaaca ttgaaattaa gaagggtgaa tcagttgtaa
     5041 tgctgggtaa gtcaggaaca ggaaagtcag ttatacttca atgtataatt ggattattac
     5101 ctccgacaaa aggtacaata aaagtattcg atacagattt tttgaaactt cctgaagttg
     5161 aaaaaaacag cataagaaga aaaattggtt ttctgtttca aagcggagca ttgtatgact
     5221 ccatgacagt caggcaaaat ctggaatttc cgataatcag acaacctgat tacaaaaaag
     5281 aagatctttc agaaaaaata actgaaatat tagaaagtgt aagtctttct gaagctattg
     5341 ataaaatgcc gtctgagctg tctggaggaa tgaaaaaaag aatcggactt gcgaggacgc
     5401 tgatgctgga tcctgaaatt attttgtatg atgaacctac gaccggtctt gatcctgtta
     5461 cttcaaacga gattagtcat ttgatacttg acatgcaaaa aaaattaaat gtgacatcac
     5521 taatagtaac tcatgatatg gcatgtgcaa aactggtagc ggaccgaatt attgtattaa
     5581 aagacggtac tattgctgcc gagggaagct ttgatgaact tgcaaaatct gatgatgaat
     5641 ttgtaagagg gttttttgaa tttgaatgat tttaaaattt tttaaataat aatcaggttt
     5701 gttctttttt taaattttgt attattctat tttattttta gataaaaaat tttaaggcaa
     5761 tttatattat aataattatt aaaaatattt taatttttaa atatgggtat taacaaatcg
     5821 aaatatatta agcttgggat attcattatt acaggacttt ttctgtttgt atttgcactg
     5881 ttttatatag gaagcaaaga aaatttattc acaaatactt ttaatgtgta ttcagtattt
     5941 tcaaatgtca gtggtctgac acagggaaat tctgtgcagt atgccggaat aaatgtagga
     6001 acagtagagt ctattgaaat actttcagtg gatcaggtac gcgtgaatat gaacctagta
     6061 agcgatgtgc agaagtttat aaaaaaggat tctaaagcaa ccataagctc agacggactc
     6121 gtcggaagta aaatagtttc gataactccg ggaaaattcg agtcagtgat ggtcgaaaac
     6181 ggtgattcaa tacattctgt caatccggta tcaatgggtg acatcattaa taatctcaat
     6241 gaatcttcaa aagaggcgga agtaattgcc aaagaattaa ctgagataat gtcaaaaata
     6301 aataacggag agggtacact aggaaaactg gttaataact caagtatgta tgacaatctt
     6361 gattccttac tgaatagttt tgcgggctct acagcacaaa taaataatat cctgactcag
     6421 acttcaaata caattgacat ggtagccggt gatattcagg aaatgcaggg aagtgttaaa
     6481 ttaattgtaa ccaatatcgg tgatattact tcaaagataa attcaagtca gagtcttgta
     6541 ggaacacttc ttacagatac tgtatttgca aataatataa aaggagtagt gaaaaatgca
     6601 aatttcacaa ctcaaaatct tgaaatggga tcattcagtt tttatcagaa tatggaagct
     6661 ttgaaacata atttcctatt caaaggatat tttgaagatc tgggatattg ggatcagtca
     6721 aaggaggaaa aggattatat ttcaagagaa gaacgtatac tcaacaaaga acaagagctt
     6781 aatataaagg aattgcagct cagggaattt caggaaaaac tggattcatt aaaaaataat
     6841 cttcagataa aaattgatac ttcaaaaaca ggtgaatcga aataattaat tatattataa
     6901 aataactttt aatgattttt tgattttcct atattgcatg attaaattta aaaataaaaa
     6961 tcttttaagg agatataaga aatgacaaac gctgaaattg aagcaaaagt agtaagtgca
     7021 gtagtagata aacttggtgt agaagaatcc aaagtaacta cggatgcatc ttttataaat
     7081 gatcttggtg cagattcatt agacacagtt gagcttgtaa tgaaatttga agaagagttt
     7141 gatattaaga ttccagatga agatgctgag aaaattcaaa aagtaggtga tgcagtaagc
     7201 tatatcacag aaaaaatatc agctaactaa ataaggtcac gttatttttt attaagtaaa
     7261 attcatgaag agaagagttg tcgtaacagg tattggtgta gtcagtccgg taggattaaa
     7321 tatcaatgac ttttggagta gccttattgc tggcaaaagt ggtgttgata atatcacata
     7381 ttttgatgca actgattttg atacaaaatt tgctgctgag ttaaaaggat ttgatccatt
     7441 aaattatatg gatagaaaac tttcacagcg tgtagatcca ttcactcaat atgcattagt
     7501 cgcatccgaa gaagctattg tagactcagg tctgaattta gaaaaaattg attctgaccg
     7561 ggcaggtgtt gtgtatggat ctgggatcgg cggaatgtgg acttaccata atcaacagac
     7621 aaatctttat aaacgggacg gtaatcctga caggatcagt ccattcttta ttccaatgct
     7681 tattgcagat attgcagcag gaagaatttc aatgaagtac ggattcaaag gaccaaacta
     7741 tgcaacaata tcagcatgta caacttcagc tcattctata gctgattccg taatgctaat
     7801 tgaaagaggt gacgcagata ttatgataac cggcggatca gaagctgtaa tttgtccaat
     7861 gggagtcggc ggttttaatt caatgaaagc actttcaaca agaaatgacg cacctcaaaa
     7921 agcttccaga ccatttgaaa aaaacaggga cggatttgta atgggagaag gcggagctac
     7981 gttaatatta gaagaactgg aacatgctaa atccagaggt gcaaatattt atgcagagat
     8041 cacaggattt ggattaacag cagatgcttt tcatataaca gaacctgctc ctgaaggtga
     8101 aggagtcgga agagctattt caattgcaat aaaaaattca aatctgacaa tcaatgatat
     8161 tgatgtcata aatgcacatg gtacttctac atattataat gataaaaatg aatccatggc
     8221 tatcaggaat gtttttaaag aaaaagctga tcagattcct gtccattcaa ttaaatcaat
     8281 gattggtcat ttactcggag ctgccggtgc tatagaagct atctcttcga ttttaacaat
     8341 tcgtgacggg atcattccgc caattataaa ctacgaagaa aaagatcctg aatgtgatct
     8401 taactacgtg gtcaatactg caatgaaaaa agatgtgaaa accgtgttat ccgagaattc
     8461 aggattcggt ggacacaaca ctgcaatatt atttaagaaa tacgaagaat aatccttatt
     8521 tgcaatttca ataatttatg tttaatcttt taaagaaccg gttaagaaaa ttaacaccct
     8581 ttttcgaaac tcgaaaagag aaaaagaatt tatctaaaaa attaactgac tttattgatt
     8641 ttgatgaact tcagaataat atccattata agatcattga tgaaaactat tttgtaaatg
     8701 ctcttactca ccgttcattt ctgacagtaa gatctgatct gtcacaagtg ggaataattt
     8761 caaatgaacg attagaattt cttggagatg cagtattaga tctcgtagtt gcagaatatt
     8821 tatacaaaaa ttttccatta aatgaagaag gtgatcttac taaattcaga tcaatacttg
     8881 taaataaaaa atttcttgct gaaagagcaa aacatttaaa acttcaggat tttcttttag
     8941 catcatacac tgcaaaaaaa tctattgatg aaggttatga tgctattctt gctgatacat
     9001 atgaagctct tattggtgcg gtattcatgg attcaggata tgaaaatgcc aaagaatttt
     9061 tgaatgagga aatttttatt aaactagata taaaatggct caatcaattt gatgaaaatc
     9121 ataaaagtaa atttcttgaa tatgtacagg cacatactga ttttattcct gaatattcta
     9181 ttataaaaga agaaggacct gagcacaata aattatttac tgttgaagtc tttgtaaaca
     9241 agcgtagtct tgggatcgga aaagggaaat ctaaaaagca ggcggaacag gcttctgcaa
     9301 aaaacgcatt aaataatctt gaagtgttgg aaaaaatggg attaacgaaa aaaaaatctt
     9361 agcgaaaaat atttgatacg gtattgattt tgagagtctc taacaatata tttgcatagt
     9421 tcataataaa aaaaataaat tcactgtata gattagtttg gtattaattt tataagtttc
     9481 ttactaaaac tctatgcatt ttattttaaa aaaccatttt ataaagaaag gtattcatct
     9541 ttac
//
LOCUS       DAORVQ010000125         9390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3964_length_9390_cov_5.712051, whole genome shotgun sequence.
ACCESSION   DAORVQ010000125 DAORVQ010000000
VERSION     DAORVQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_9390_cov_5.712051"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLX80_10100"
     CDS             <1..724
                     /locus_tag="PLX80_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY21182.1"
                     /translation="NLEVLGAKVNKIKFENQIRNFSSEIRSLQSEIRNLMNVDYNISP
                     VGEFVFRDLIISRTALVQNAVNNNPALKMMKYQKEKFSNKISLSKGELLPDISFKYFR
                     QKLGSDNNYWGMELGLGVPLWFWWEPTGKIKESNYEFQIASSEEINLRNSLENQINQA
                     FENYENNSRQLNFFNEEVLRETEEILRQSKISYEQGEIGYVEYLQALNLAYETRTQYL
                     NAIFNYNQSIINLEKLTAGEIK"
     gene            721..2088
                     /locus_tag="PLX80_10105"
     CDS             721..2088
                     /locus_tag="PLX80_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY21183.1"
                     /translation="MKNNLIILIINVLLILAVSGCSNKDEATNQSKDMHSEESAHNEE
                     GEQHEDEIALSEEQFKLMGIEFAKYEKQNIEGYIKANGEIMINPDNESKVGGIIPGRV
                     RKIYVKEGSFVRAGQTLAVIENPDLINVQVDYINAKNDFEFAKQEYDRQKKLNSDNIG
                     SKKNLAEIETNYKRALANYKTLEEKLSVYRISKNRFDNIYQDTIANLQRNFSITAPIS
                     GSIVSRKITVGQYVEPSMDMFYIVNTSTVFVDLSIFEKDLQYISIGQKVLIETSKNTT
                     KEYEGNISFINKVFDDKNRTVKVRVSINNKAQELYPFMFVTAKIYVDDGNVLAVPVSA
                     IESEGESKYIFVKTDERRKIETHEEQKEGDEQKEGDEQNEGEEQKEAEEQKENEKHEE
                     DEVHSGEEGIVFKKVQVITGISDDKYVEIFPIEELKDGAEVVSKGTFYLKSELKKDEL
                     GEDED"
     gene            2134..2337
                     /locus_tag="PLX80_10110"
     CDS             2134..2337
                     /locus_tag="PLX80_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21184.1"
                     /translation="METTKFFVSDFHKGTVNIILHVISFTIMFYGLVIKDTIFLILGL
                     AVINELSHLFNYFILYKRNPNMA"
     gene            2468..2905
                     /locus_tag="PLX80_10115"
     CDS             2468..2905
                     /locus_tag="PLX80_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21185.1"
                     /translation="MKTRLSMVLIFFVTLNFIFSGYVYERDEQVLNDSYFQIEIQDTV
                     KSRYYEAPDGELPFGYFKIIDNGSIIVYSQKAHHPRYGSYLKYYYSINLKSRIHELTL
                     ENLEVDFTDNPKFIKLIKESKKLWQTMTNGITYLNKLWLESLK"
     gene            2984..3208
                     /locus_tag="PLX80_10120"
     CDS             2984..3208
                     /locus_tag="PLX80_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21186.1"
                     /translation="MKKLSKQIIAFFLAGFFAKDIIDDIFFLVLLTDKYPLEIFGFSI
                     TATSHKIMLVVSTIMTAVFFYYGLKKTQNN"
     gene            3506..3835
                     /locus_tag="PLX80_10125"
     CDS             3506..3835
                     /locus_tag="PLX80_10125"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HQY21187.1"
                     /translation="MKHNFVAINFIDCEEAYKERFELLFTNRAHAIDRMKGFKNMEVL
                     KPKDGTSSYLVVSYWESEADFKKWKDSPEFLEGHRRGFEDISKAKMEGRKPPMKSSFK
                     TYEILAR"
     gene            4748..6388
                     /locus_tag="PLX80_10130"
     CDS             4748..6388
                     /locus_tag="PLX80_10130"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQY21188.1"
                     /translation="MTGKEKELYKTYEPIFSDFTDPPPTPVRTMSEWEEFQGILITWT
                     SYTTILRQMVDYAQDEGLVFIVCSDSSSVKSFLTSGGVPLVNLKFLITSFNSVWSRDY
                     GPWSAYSGVADSLKIVDWVYNRPRPLDDVISVYFANYANLPIYQATQAPNDLISPGGN
                     FLHDGNGTAFSSKLVLDENPTHTEAEIDTLVRRYNGIKRYIKMNTLPYDGIHHIDMHM
                     KVLDEETLLVGQYPTGVSDGPFIEANLQYILNNFPTCYGRPYKVVRIPMPPSLTGQYP
                     PSSSYYTYTNSLFVNKTMMVPIYGFPQDTTALRIYRENLPGYRVVGINCSSMISAGGA
                     LHCIAKEIGAVEPIFISHAKLYNTNNTVLPYEVKAYIKTKSNVSGAKVYWSTDTSVGF
                     NQLTMTALASDTFRTFIPAQPLGANVYYYITANSNSGRTISKPLTAPGGFYQFRIDNP
                     TVINNGQQVYTFALNQNYPNPFNPSTSIGFEIPAFGFVKLKVYDLLGKEIATLINEAK
                     HAGNYKVKFSDSDLSAGIYFYRLEVNNMVEIKKMILLK"
     gene            6471..8627
                     /locus_tag="PLX80_10135"
     CDS             6471..8627
                     /locus_tag="PLX80_10135"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21189.1"
                     /translation="MKKKIQLSLIFLFSIFIFSNTVFSVGFNSIHTPDGVYIIAVGDA
                     GKIFRSSNSGNTWASYTMASVNFKSVYSFNNNIWISGDNGKIYKTLKTNSAITDIDLG
                     ITTSINSIYFLNDNDGYLCGNSGIVYKTNNGGTNWAPCNTGISSVNLNSISFMDSQKG
                     IIVGNNGSVYVTVDGGTTWTPEAVGTTKNLTKGKYFTDGIAVVGEYGSLYIKNGTTWS
                     SVNTRIVTDIRGVTGTSINDVHICGGGGFIRNNKSNDTRFYTFEANPMVANLVDIFYY
                     DTNLGFAVSSLNDAIIKTTNGGELWSLTAGAAMTYQWQSKLSAGSGIGNNLCPHPFNR
                     DAMYVVYGSTVYISRNRGENWSSIASITGGGSAHSFYVSPLDTNVMMVAITGSPDKIM
                     RSSNYGQTWTTIISRNFSNYGQPLEMDQNNPSTYYFAPDNGGFYRSTDNGLTFTEISN
                     NFPFRSPCDLLVMWDSSNVVFVGDGVTGSGQAKIFKSVNGGVNWTDVMTVSTSETPSM
                     CNGVFEQANVYATEWSGSNWYESEDYGSTWHVDFNTGFSGWASGVCFEDPNVILIGNY
                     GSQSAFTFNGQDYTNISGLSGAGAGLMIFEKNMIINMQTSGLWKLKVNYSVLTSVNEN
                     VYTTQIPNDFNLYQNYPNPFNPTTNIKFDLPRAGNISLKVYNELGKEVETLADGFRSA
                     GSYEINFNASALSSGIYFYKLTTNNASIARKMLLVK"
     gene            8817..9152
                     /locus_tag="PLX80_10140"
     CDS             8817..9152
                     /locus_tag="PLX80_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21190.1"
                     /translation="MTAALGLLSIYNRQSYVINNFGQEYSFELSQNFPNPFNPSTTVS
                     FEIPTMGVVSVKVYDVSGKEVAILLNEKKHAGRYEINFNASNLSSGIYFYRLEFDGNV
                     RFKKMTLLK"
     gene            complement(9317..9390)
                     /locus_tag="PLX80_10145"
     tRNA            complement(9317..9390)
                     /locus_tag="PLX80_10145"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9354..9356),aa:Asp,seq:gtc)
BASE COUNT         3345 a         1445 c         1733 g         2867 t
ORIGIN      
        1 aaatcttgaa gtactgggcg caaaggtaaa caagatcaaa tttgaaaatc agatcaggaa
       61 tttttcatcg gaaataagat ctttacagtc ggaaataaga aatcttatga atgttgatta
      121 taacatatca cccgttggtg aattcgtatt cagggattta ataatttcca gaactgcact
      181 ggttcaaaat gccgtaaata ataatcctgc tttgaaaatg atgaaatatc agaaagaaaa
      241 attctcaaat aaaatttcct tatcaaaggg agagctgctt ccggatatca gctttaaata
      301 tttcagacaa aagcttggaa gcgacaacaa ttactgggga atggaattag gactgggtgt
      361 tccgctctgg ttctggtggg agccgaccgg taaaataaaa gaatctaatt atgaatttca
      421 gatcgcatca agcgaagaaa ttaatctgag gaattcatta gaaaatcaga ttaaccaagc
      481 atttgaaaat tatgaaaaca attcaaggca attaaacttt ttcaatgaag aagtattaag
      541 ggaaacagaa gaaatactca gacaatcaaa aataagctat gaacagggag agatcggtta
      601 tgtggaatat cttcaggcat tgaacctggc ttatgaaaca cggacccaat atctgaatgc
      661 aattttcaat tataatcaat caataattaa cttagaaaaa ctcaccgcag gtgaaataaa
      721 atgaaaaata atctaattat cttaataata aatgtactgt taatacttgc tgtctcagga
      781 tgcagcaaca aagatgaagc aactaatcaa agcaaagata tgcattccga agaatcagct
      841 cacaatgaag aaggcgaaca acacgaagat gaaattgcat tgtctgaaga acaatttaaa
      901 cttatgggaa ttgaatttgc taaatatgaa aaacagaata tagagggcta tataaaagct
      961 aacggtgaaa taatgataaa cccggataat gaatcaaagg ttggaggcat tattcccgga
     1021 cgagtcagga aaatttacgt aaaagaagga tcatttgtga gagcagggca aacactcgcg
     1081 gtaattgaaa atcccgatct gataaatgta caggttgatt atataaatgc aaaaaatgac
     1141 tttgaatttg ccaaacagga gtatgatcga caaaagaaat taaatagcga caatatcggc
     1201 tctaaaaaaa atctggccga aattgaaaca aactacaaac gcgcacttgc aaattataaa
     1261 acgcttgaag aaaaactgtc ggtttataga atctcaaaga acaggtttga taatatctat
     1321 caggatacta ttgcaaatct tcaaagaaac ttttcaataa cagctccaat ctcaggtagt
     1381 attgtttcaa ggaagataac ggtcgggcaa tatgtcgagc cttccatgga catgttttac
     1441 atagtcaata cctcaactgt ctttgtggat ctgagtatat ttgaaaaaga tttacagtat
     1501 atcagcatag ggcaaaaagt cttaatagaa acaagtaaaa ataccactaa agaatatgaa
     1561 ggtaatatct cctttataaa taaggttttt gacgacaaaa acaggactgt aaaagttaga
     1621 gtatcgataa acaataaagc acaggaactt tatcccttta tgtttgtaac ggcaaaaatt
     1681 tatgtagatg atggaaatgt attggctgtt ccggtttcag caattgaatc cgaaggagaa
     1741 agtaaatata tttttgtaaa aacggatgag agaagaaaaa ttgaaacaca tgaggaacaa
     1801 aaagaaggtg acgaacagaa agaaggtgac gaacaaaatg aaggtgagga acagaaagaa
     1861 gctgaggaac agaaagaaaa tgagaaacat gaagaggatg aggtgcacag cggagaagaa
     1921 ggtattgtat ttaaaaaagt tcaggtcatc actggtattt ccgatgataa atatgttgag
     1981 atatttccaa ttgaagaatt aaaagatggt gcagaagtag tatccaaggg aacattttat
     2041 ttgaaatcag aactaaagaa agatgagctt ggcgaggatg aggattagaa ggaattaaaa
     2101 tatgttattc tcacttttta aattatgaat tttatggaaa ctacaaaatt ttttgtttca
     2161 gattttcata aaggtacagt caatatcatt ttacatgtaa taagttttac aatcatgttt
     2221 tatggattag taatcaagga cacaatattt ttaatactgg gactggctgt gatcaatgaa
     2281 cttagtcact tatttaacta tttcattctt tacaaacgga atccaaatat ggcataagaa
     2341 tggtgcccaa tcaaattcta tacgcatcga taattcttgt aattttgttg gaagtattta
     2401 attggtatta agttaacata tttataatta gtaaacaaaa tctctatcta aaattaaaaa
     2461 aataaaaatg aaaactcgct tatcaatggt tttaattttt tttgttaccc taaatttcat
     2521 attcagcgga tatgtatatg aaagagatga gcaggtatta aacgatagtt attttcagat
     2581 agaaatccag gataccgtca agtcccgtta ttatgaagct cccgacgggg agttgccctt
     2641 tggttatttt aaaataattg ataacggttc aattattgtg tattctcaga aagcgcacca
     2701 cccgcgatat ggaagttact taaaatatta ttacagcata aatttaaaaa gcagaataca
     2761 tgaactgact ctggaaaatc tggaagttga ttttactgac aatcctaaat ttattaaatt
     2821 gattaaagag tctaagaaac tttggcagac gatgacaaat ggcattacat atttaaataa
     2881 attgtggctt gaatccttaa agtaataagt aagtttagaa atgatataag aactattgaa
     2941 acatgttcac attcaatcaa tctcaagcaa aaaattttac ttaatgaaaa aactatcaaa
     3001 gcaaatcatt gcattttttc tggccggttt ctttgcaaaa gatattattg atgacatttt
     3061 ctttttggta ctattaacgg ataaatatcc tcttgaaatt tttggatttt caattacagc
     3121 aacatcgcat aaaattatgc tcgttgtatc aacaatcatg acagcagttt ttttttatta
     3181 cgggttaaaa aaaacacaga ataattaaat atgaaaaaac taaaatttat tatgctgctc
     3241 accttcgttt attaaataag ggtttagcgc aggacagaca ttatggatct agttataata
     3301 gccgtaatag tgtggttggc agtatttaat taattggtca cctgtataac tatttcattc
     3361 ttttcaatcg gaagccaatg ctttagtaaa tgggcgctta gttaatacgc ggacttaaaa
     3421 gggattaacc cttcaagaca atgaacagga aaaacataaa atttaatcaa gtaatcacaa
     3481 taattttaat ttaaaacaag taagtatgaa acacaatttt gttgctatca atttcattga
     3541 ctgcgaagaa gcctataaag aaagatttga actattattt acaaaccgtg ctcatgctat
     3601 tgatcgcatg aaggggttta agaacatgga agttttaaaa ccgaaggacg gaacaagcag
     3661 ttaccttgtt gtaagctatt gggaaagcga ggccgatttt aagaaatgga aagactcacc
     3721 agaattttta gaaggacaca gaagaggatt tgaagacatc tcaaaagcca agatggaagg
     3781 aaggaagcct cccatgaaat cgagctttaa aacttatgaa atattggctc gatgagtata
     3841 agcgaaaaat tttttgactt aaaaagtaaa gggtggttca gaaaaattgg gattgctgct
     3901 tttttatttt ttctcattaa gggtttgata tggataggaa tcttaatctg gggatatttt
     3961 gcgtttctaa aataatttac gccataatga aaggatggtt gttgtatcga aagaaatgtt
     4021 ttatttaaga ttgcttaaaa gaaatgaact tgtcgaaatg agcataaaat ataaaaagag
     4081 tttactgaaa tgaattttat ttcaatagaa aaaatttcta ccagagaagc gaggaaaata
     4141 aggtggatcc ttttcggttg tatagcattg gctttcgtct caataaagtt tccccttccg
     4201 aaattcgata taatgttaaa tttatttggc atcccctgcc cattctgcgg aatgtccaga
     4261 gcatttattg aatttattaa tttaaacttt tccaaaagta tttattacaa tccttcatct
     4321 gtgattttat ttccattttt agtttcagct gctttaagtg tttttatgtt aagtatattc
     4381 aataataaag ttttgattaa atttaattat aaaacttttt ctatcgcggc tatattcatt
     4441 ttgttaatgt gggttttgaa tatttttatt gggcatcatt aagtttaatg ttaacaaaag
     4501 tattaataca gaatgctata ttttaagatg ttgttttgaa gtttattgat attagatccc
     4561 atataacgag atactactat gaaaaataaa aagattgaat ttagacaata aatttaaaat
     4621 tttaaatata tcctatattt ataaaacggt tttaataaaa taatatttga caatgaaaaa
     4681 attattatca cttattttat ttgtttcttt cgcgagtact actttttccc aggatcttcc
     4741 gcgtcatatg acaggcaaag agaaagaatt atataaaact tatgagccga ttttctcaga
     4801 ttttactgat ccgccgccta caccagtgag aactatgagt gagtgggagg aatttcaagg
     4861 gattttaatt acatggactt cttatacaac aattctgagg caaatggtag attatgctca
     4921 ggatgaggga ctagttttta tcgtctgtag tgattcaagc tctgttaaga gttttctcac
     4981 cagtggtgga gtaccattag taaatttgaa atttttaata acttctttta attctgtgtg
     5041 gagcagggat tacggaccct ggtctgcata ttccggtgtt gccgatagct taaaaatcgt
     5101 tgactgggtt tataacagac caagaccatt agacgatgta atttctgttt attttgcaaa
     5161 ttatgccaat ctcccaattt atcaggcgac tcaggctccc aacgatctaa tttcgcccgg
     5221 tggaaatttt ttgcacgatg gaaacggtac ggcattttcg tcaaagcttg tattggatga
     5281 gaaccctact cacaccgaag cagaaattga taccttagtc cgaaggtata atgggataaa
     5341 gagatacatt aaaatgaata ccttgcctta cgatggaatt catcatattg acatgcatat
     5401 gaaagtctta gatgaggaga ctttattagt aggacaatat cctactggcg tttcagatgg
     5461 accatttatt gaagctaatc tccagtacat attgaataat tttcctacgt gttatggaag
     5521 accttataaa gttgtaagaa taccaatgcc accaagttta acgggtcaat atccacccag
     5581 ctcaagttat tatacttata caaattccct ttttgttaat aaaactatga tggttcctat
     5641 ttatggattt ccacaggata caactgcatt aagaatttat agagaaaact tgcccggtta
     5701 ccgggtagta ggaattaatt gtagttctat gatttctgcg ggaggagcac ttcattgcat
     5761 agcaaaagaa ataggtgcag tagaacctat ttttatttct catgcaaagc tttataacac
     5821 aaacaacaca gttttgccat atgaggtaaa agcatatatt aaaacgaaaa gtaatgtctc
     5881 aggtgcaaaa gtttattgga gcactgatac atcagtggga ttcaatcaat taacaatgac
     5941 agctttagcg agcgatacat tccggacttt tattcctgct caacctctgg gagcaaatgt
     6001 gtattattat attaccgcaa actcaaacag tggaagaaca atctcaaaac ctttaactgc
     6061 tccgggaggt ttttatcaat ttagaattga caatccaaca gtaattaata acggacagca
     6121 agtatatacc tttgcgctaa accaaaatta tcctaatccc tttaatccta gtactagtat
     6181 tggatttgaa atacctgcat tcggttttgt aaaattaaaa gtttatgacc tattaggaaa
     6241 agagatagcc acgcttataa atgaagcaaa gcatgccggc aattataaag taaaatttag
     6301 tgattccgat ttatctgctg gaatatattt ttatagactg gaagtgaata atatggttga
     6361 aattaagaaa atgattctct tgaaatgaag atattccata ataaaatttt tattgtaatt
     6421 tcaagtataa ctaattaatt cacatattta aaaatcaaaa tccaaaaaac atgaaaaaga
     6481 agatacaact ttctttaata tttttgtttt caatatttat tttttcaaat acagtttttt
     6541 ccgtcggttt taacagcatt cacacaccgg atggagttta tataattgct gttggcgatg
     6601 caggcaaaat tttccgttca tcaaattccg gaaatacctg ggccagctat acaatggcca
     6661 gtgttaattt caaatcggtc tattcattta ataataatat ctggatttca ggagataatg
     6721 gaaaaattta taaaactctg aaaacaaatt ctgcaatcac tgatattgat ctgggaatca
     6781 ctacctcaat aaactcaatt tatttcttaa acgataacga tggatacctt tgcggaaaca
     6841 gtggtattgt ttacaaaaca aataacggag gaactaactg ggctccttgt aataccggaa
     6901 tttcttcagt caatctgaat tcaatttctt tcatggattc tcaaaaggga attatagtcg
     6961 gtaacaacgg atctgtttat gtaaccgttg acggaggtac cacatggact cccgaagcag
     7021 tcggaactac caaaaattta acaaaaggaa aatattttac ggacggaatt gcagtagtag
     7081 gagaatacgg atcattatat attaagaatg gcactacatg gtcttcagta aatacaagaa
     7141 ttgttactga cataagaggc gtgacaggaa caagtattaa cgatgttcat atctgtggcg
     7201 gcggcggttt catcagaaat aataaaagta acgatacacg attctataca ttcgaagcaa
     7261 atcccatggt tgcaaattta gtagatatat tttattacga cacgaattta ggttttgcag
     7321 taagcagcct taacgatgca attattaaaa caacaaatgg tggtgaactg tggtcattga
     7381 ctgcaggagc agccatgaca tatcagtggc agtcaaagct aagcgcaggc agcggtatcg
     7441 ggaataattt gtgtccgcat ccgtttaaca gagatgcaat gtatgttgtt tacggaagca
     7501 cagtttatat cagcaggaac aggggtgaga actggtcttc tattgcttca atcacaggcg
     7561 gtggtagcgc tcattcattt tatgtaagcc cgctcgatac aaatgtcatg atggtcgcaa
     7621 tcacaggaag tccggataaa attatgcgtt catcaaatta cggacaaact tggacaacca
     7681 tcatttccag aaatttcagc aactacggac agccgctcga aatggaccag aataatccaa
     7741 gtacatatta cttcgcaccg gacaacggcg gtttctatag atcaaccgat aacggattaa
     7801 ctttcacgga gatcagcaat aactttcctt ttagaagtcc ttgtgatctt cttgtaatgt
     7861 gggacagttc gaatgtagtt tttgttggtg acggggttac aggaagcggt caggcaaaga
     7921 ttttcaaatc tgtcaatggc ggagtaaact ggacagatgt tatgactgtc agcacaagtg
     7981 agacaccatc aatgtgcaac ggagtatttg agcaggcgaa tgtttatgca acagaatggt
     8041 caggttcaaa ttggtatgaa agtgaagact acggaagtac atggcatgtt gattttaaca
     8101 ccggtttcag tgggtgggca tctggagttt gctttgaaga tccgaatgta attctaattg
     8161 gaaattacgg ctcacaatcc gcgtttacat ttaacggaca ggattacaca aacattagcg
     8221 gactcagtgg tgccggagcg ggtttaatga tatttgaaaa aaatatgatc atcaacatgc
     8281 agacaagtgg tctctggaag ctgaaggtaa attacagtgt gcttacttcc gtcaacgaga
     8341 atgtttacac tactcagatt cccaacgatt ttaatttata tcaaaactat ccgaatcctt
     8401 ttaatccgac cacaaatatt aaattcgatc ttccaagagc agggaatatt tctctgaaag
     8461 tttataatga acttggtaaa gaagttgaaa ctttagctga cggtttcaga agtgcaggaa
     8521 gttatgaaat taatttcaat gcatcagcat tatcttcagg aatttatttt tataagctga
     8581 cgacaaataa cgcttcaatt gcaaggaaga tgcttctcgt taaatagtaa gtattaagtt
     8641 aagtgataag taatacgaat tcaggggttg aaaatttttt caacccctgt ttttttgaaa
     8701 agaataaaaa ttcaagatga ctgaaaccta aatacccatc aatccaacct ctcggaacaa
     8761 atatttatta ttatatttct gcaaactcaa acagcgggag aacaattaca aaacccttga
     8821 cagctgccct ggggttatta tcaatttaca atagacaatc ctacgtaatt aataattttg
     8881 gccaggaata tagctttgag ctgagccaga atttccctaa tccatttaat ccaagtacaa
     8941 ctgtttcgtt tgaaatacca actatgggtg tagtttctgt aaaggtttat gatgtatcgg
     9001 gaaaagaagt tgctatactt ttgaacgaaa agaaacacgc cggtcgatat gaaataaatt
     9061 ttaatgcttc aaatctatca tcaggaatat atttctatag attggaattc gatggtaacg
     9121 ttaggtttaa gaaaatgaca ctcttaaaat agtacttaaa atttttaaag ttcaattaat
     9181 aagtattggc tttacattta tcgactcaag aatgaattga aaaacctacc caaatcctgc
     9241 ccgccgagca tagctatgaa attcttttaa ataaaaaaac cctttaagtt cttatactta
     9301 aagggcttac tttttgcgga atcgacgaga ctcgaactcg cgacctcctg agtgacagtc
     9361 aggcgttcta accaactgaa ctacgactcc
//
LOCUS       DAORVQ010000126         9359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3984_length_9359_cov_5.404880, whole genome shotgun sequence.
ACCESSION   DAORVQ010000126 DAORVQ010000000
VERSION     DAORVQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_9359_cov_5.404880"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..634
                     /locus_tag="PLX80_10150"
     CDS             47..634
                     /locus_tag="PLX80_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQY21191.1"
                     /translation="MESFKNFILDGLLFLLPVGLMIFLLNFLLGIITEIVTKVSNFLP
                     QYSTFGQEMPKLSALIILLLLVIFSGWLSRSALGVMLGNKIEAIVSKTIPGFSVVKQL
                     FMDPKEMFEDKKIKSALALIDDAWLFAFILEEKNDADLIVVFVPSSPLPTSGNVYLMK
                     EEQIKRLDISVKETVTCITQLGIGSGKILEGKIPK"
     gene            complement(690..1079)
                     /locus_tag="PLX80_10155"
     CDS             complement(690..1079)
                     /locus_tag="PLX80_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQY21192.1"
                     /translation="MISDIKIYNEKQTQTDKEICDLLASNIDSVLKEAESKIWHAHPV
                     WFLDGNPVVGYSKLKDSVRLLFWSGQTFEEEKLEDLGKFKSADIRYTSKDLIKLTDLK
                     RWLKKSREIQWDYKNIVKRKGKLIRLK"
     gene            complement(1114..1773)
                     /locus_tag="PLX80_10160"
     CDS             complement(1114..1773)
                     /locus_tag="PLX80_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQY21193.1"
                     /translation="MYDLPYHKEKNETVIKEFIDANPFAYLSGCDKDNKPAATQVPVF
                     IEEENGRKFLRGHIMKNTDHHKAFMHNPNVLAVFSGHHTYVSGTWYSDPHLPSTWNYM
                     SVHAKGVIKFLDDEALISILRKTSMHFENYNKMSSTVFDNLDKEFKERMMKAIVAFEV
                     EVIKLDNVFKLSQDRDAESYENIKKELTKQGGSGKIIADEMEKRTKGLFPDTSELKTN
                     F"
     gene            complement(1894..2940)
                     /locus_tag="PLX80_10165"
     CDS             complement(1894..2940)
                     /locus_tag="PLX80_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQY21194.1"
                     /translation="MKIGMVVNDILTEQPGYTTTRLGIAGINRGHEVYLMGLGDMAYD
                     ADEKIRARARTVPKKKFKSNESYLKALQGKHAIKDRITVDELDVLLLRSDPASETGTR
                     SWAATAGINFGRIAMRHGVIVLNDPNGLAKAVNKTYFQLFPEEVRPKTLITRDVKEIK
                     EFASEHGGKMVLKPLQGSGGSGVFLVRPEDKSNLNQIVESLLRDGYIIAQEYLPAAND
                     GDIRLFMMNGQPMKYKGKYAAFRRIRHGDDMRSNVHAGGKLAQAVITDEHLRLAEIVR
                     PKLVQDGMFLVGLDIVGDKLMEINVFSPGGLGSAQIFEKVNFNNSVIDALETKVAYMN
                     YYKRKFDNVEMATL"
     gene            complement(2937..4844)
                     /locus_tag="PLX80_10170"
     CDS             complement(2937..4844)
                     /locus_tag="PLX80_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HQY21195.1"
                     /translation="MIKPVINTISDEFINQVCSRLAKNKRVRRSLPEWGRIHIDRQLP
                     FICIYRYNEVDKYSLSDSLVTGEASYLTAKDTSVRHKQLLKLIKSIASTLKESFGTFL
                     IVEVWVSNDKDLNNELNAYQGSFKINISKGTEILPTVQSLERSLRNIKLRKEKSEVEV
                     ITVPRIAPKELSPVISFSEARKLGIHIMGIEVKPIYFNVETEKVYPILLRNLRKGFSR
                     ALSNGFFEFTKNNTPFKPLNFQSLGKRSMGKAVWEVDRQLAEINKGFDFLLQVTPYNI
                     DYAYSQFKRNHFEKVPLFLYRPLTLDPSLAKRSLFHIPIENIEDPTLAQLFLEQQLEL
                     DRKFSMLSDRGSSSFLYGSMQLYGTVDDSLMNVAKDILQQLSNRARDGFKSKKYNAKD
                     FAKRAEIELNYFREKLPDIKTKISIRKDITGLMVSQGNLLIGERLKVPVERVEALIAH
                     EVGTHVLTYLNGKSQPLKQLYIGLSGYDELQEGLAVLSEYLVGGLTVSRLRLLAARVV
                     AAHHLVSGAGFIEIFRELNLDFGFELSTAFNITSRTFRSGGLTKDAIYLRGLVNLLLY
                     IKRGESLKPLFAGKISADNVAMIKELQARNVLHTVPLLPAYFNFPATEEKLRKLRNGQ
                     TLINLTKRGEN"
     gene            complement(4845..5633)
                     /locus_tag="PLX80_10175"
     CDS             complement(4845..5633)
                     /locus_tag="PLX80_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HQY21196.1"
                     /translation="MNQNKFWNLTFSAGPVVAAAIHDGRELRDDVSEIMKLSSDDRLR
                     EEDPYTGEWTSVGDTKIIVSRSRFEVDLNRPREKAVYQKPEDAWGLDIWKRDLPENIV
                     SDSLSQYDLFYKEVYEIFSTLEKQFGHFAVLDLHSYNHMRNGTDGEHADPKLNPEVNI
                     GTGTINRNKWGKLIDRFISDLHNFDFDGRKLDVRENIKFKGGQFPKWTHQTFPESACS
                     ISIEFKKFFMDEWTGKPDVCQIEKIGMALQSTVPGILEELKYIR"
     gene            5995..6690
                     /locus_tag="PLX80_10180"
     CDS             5995..6690
                     /locus_tag="PLX80_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HQY21197.1"
                     /translation="MKIESMNWSDVENYLKNNDKCILPVGSTEQHAYLSLSTDNILAE
                     KISVDAAAPLNIPVFPVINYGHTPLFMAYPGTVSISIETLSLLLKDILNSLSTQGFKK
                     ILIVNGHGGNMPLEKHVNEWSAEHENVKVKFHNWWKAPKVWDFIQQTDPESSHASWME
                     NFEWTRLKDVKLPDHKKPMVNFELLKNASPKEAREILEEGNYGGYFNKDDAILNELWD
                     VAVGETRELLNEW"
     gene            complement(6894..>9359)
                     /locus_tag="PLX80_10185"
     CDS             complement(6894..>9359)
                     /locus_tag="PLX80_10185"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21198.1"
                     /translation="NGARFSGRIIDIEVGNTANPLLATASGGLWGYLGFLPVGISEDI
                     TSLAIGSVASYPGSPSTILIGTGEPSQRSGTGMWRTTNSGSTWSHINISPDPGRFYKI
                     RHQSFFAHIVHAASTEGYYRSTDGGLNWIRHLSPANSVGISDIAINLNSPNTIYAARC
                     GDGIYKSTNNGVNWAKVTTPGIPTSDIGRTSLSVGTSNSNKIYVMIDSQAGDTLKGIY
                     MSANDGATWSDVSPGTNIFKNQGWYNNVLEVCPTNSNIVLAGGVRLWRSANSGTTWTE
                     LTDDDIHADQHAIEWTSDGQNVYIGNDGGVIYSNDQGVTFSSGINYIPITQYVNFDVG
                     ESNRGVIYGGSQDNGITGTTNGGTTWNFTKGGDGGGVSIDPISSLYVNVTAGYYSGDH
                     SFEIYRSSDQGFTWTEDHTGIDPTSTWYTKMRSDRTNPVKLYTNGGAYVYESTNAGNS
                     WNKLNVSAFPVSVTNIGVSKYVAPKAIIYATLNSFTTGQRLRVYDGGNFFERTSGIPS
                     NLRIRQVIPHLTNTTTAYALINGFSAGNKVFKTTNKGVNWTNISGNLPDVPLGGLVPH
                     RLLNNYLYLGTEMGCYRTTNAGVTWHRWNNGMPDATIITEMKWIDSTLENGKRYVIAS
                     SYGRSFWVRDVSGDDPDNELNLTMFIQGFYNPGTESTVRDTVNVYLRNSVSPYSKVDS
                     AKVYISNTGGATMTFPNAFPDNEYYVQLRHRNSIETWSSGTINFDDPTESYNFTTFPQ
                     QAYGDNQILVNPNNIRYGIYNGDADQNGLVDITDQSLVDNDLFNFTAGYVNTDMNGDN
                     FTDASDAALVDNNSFNFIGKVIP"
BASE COUNT         2780 a         1817 c         1515 g         3247 t
ORIGIN      
        1 aatcaattta taataattca atttataaaa aattatgcca actaaaatgg aatcattcaa
       61 aaattttatt cttgatggat tactctttct gcttcctgta ggtttaatga tctttctttt
      121 gaattttttg ctgggaataa taactgaaat tgttactaag gtttcaaatt tcttacctca
      181 atattcaaca tttggacagg aaatgccaaa gctctcagct ttaattatac ttttactttt
      241 agttattttc agtggatggc tgagccgatc agcattagga gtgatgcttg gaaacaagat
      301 tgaagctata gtctcaaaga ccattccggg attttctgtg gtcaaacagc tttttatgga
      361 tccgaaggaa atgtttgaag ataaaaaaat taaatctgca cttgctttga tcgatgatgc
      421 ctggctgttt gcatttattc ttgaagaaaa aaatgatgca gatttaatag ttgtatttgt
      481 cccgtcttca cctttaccta ccagcggtaa tgtatattta atgaaagaag aacagatcaa
      541 gcggctcgac atttctgtta aggaaactgt aacatgtatt actcagcttg gaattggttc
      601 aggtaaaatt ctcgaaggca aaattccgaa atagagtata tatctttttt taagaacttt
      661 tgaaataaaa cagatttact ttttaaacat tattttaatc ttataagctt accttttctt
      721 ttgacaatgt ttttataatc ccactgaatt tctctggact ttttaagcca tcgtttcaga
      781 tcggtaagtt tgatcaggtc ttttgaagtg tagcggatat ctgctgattt aaattttcct
      841 aaatcctcaa gcttttcttc ttcgaatgtc tgcccgctcc agaacagcaa ccgcacactg
      901 tctttaagtt tgctgtatcc gactaccgga tttccgtcaa gaaaccagac cggatgtgca
      961 tgccagatct tactctcagc ttcttttaaa acactgtcaa tattcgaagc gagtaggtcg
     1021 cagatctctt tgtcagtttg tgtttgcttt tcgttgtata ttttaatatc tgaaatcata
     1081 atatcaataa gttactttta atttgaataa tatttaaaaa tttgttttaa gttcactcgt
     1141 gtccggaaat aatcccttag tcctcttttc catttcatca gcaatgatct ttccactgcc
     1201 tccctgtttt gttaattcct ttttgatatt ttcataactt tccgcatccc tgtcctggct
     1261 tagtttgaat acattgtcca gctttatcac ttccacttca aaagcaacaa ttgctttcat
     1321 catcctttct ttaaattctt tatcaagatt gtcaaaaaca gttgaagaca ttttattata
     1381 attttcaaaa tgcatagatg tttttctgag aatactgatc aatgcctcat cgtcaagaaa
     1441 cttaattaca cctttggcat ggacagacat gtaattccag gtagaaggca gatgaggatc
     1501 gctgtaccag gtgccgctga cataagtgtg atgacctgag aatacagcca gtacattcgg
     1561 attatgcatg aatgctttat gatgatcagt atttttcatg atgtgtcctc tcaaaaactt
     1621 tctgccgttc tcttcttcaa taaatacagg cacctgggtt gctgcaggct tattatcttt
     1681 gtcacatcct gataaatatg caaaaggatt tgcatcaata aattctttaa ttactgtttc
     1741 atttttttct ttgtgatagg gaagatcgta catatacttt aatatttatt aagtttaaaa
     1801 aaatatatca aaactgaaat ttcttgaaat aaaattaatt acatcatatt tcaaaacttt
     1861 ttttaaaata atattatctc ataaagtcta caattacaat gtcgccattt caacattatc
     1921 aaattttctt ttgtaataat tcatgtatgc aactttagtt tcaagtgcgt ctattacgct
     1981 attgttgaaa ttaacttttt caaagatctg tgcacttcct aagccacccg gactgaatac
     2041 atttatctcc attaatttat caccgacaat atcaagacca actaggaaca ttccatcctg
     2101 aactaatttt ggtcgcacta tttcagcaag tcttaaatgt tcatcagtaa taactgcctg
     2161 agctaatttc cctccggcat gaacattact tcgcatatca tccccatgcc gtatcctacg
     2221 aaatgctgca tatttacctt tgtatttcat tggctgtccg ttcatcataa aaagtctgat
     2281 gtctccgtca ttggcagcag gtaaatattc ctgtgcaata atatatccgt ctctgagaag
     2341 tgattcgaca atctgattca gatttgattt atcttcaggt cttaccaaaa aaactccaga
     2401 tcctcctgat ccctgcaaag gttttaacac catttttcct ccatgctctg aggcaaattc
     2461 tttgatctct tttacatcgc gtgtgatcag agtttttggt ctgacctctt ctggaaagag
     2521 ctggaaataa gttttgttta cagcttttgc aagtccgttt gggtcattca aaacaataac
     2581 tccatgacgc attgcaattc ttccaaaatt aatacctgca gttgcagccc atgaccgggt
     2641 acccgtttcg cttgcagggt cacttcgaag cagtaaaaca tcaagttcat caactgttat
     2701 ccgatctttt attgcatgtt ttccctgaag tgcttttaaa tatgattcgt ttgatttaaa
     2761 ttttttttta ggtacagtac gtgctctggc gcggattttt tcgtcagcat cataagccat
     2821 atctcctaat cccatcaggt atacttcatg tccgcggttt attccggcta tacctaatcg
     2881 agtggttgta tatccgggct gctccgtaag aatatcattt actaccattc caattttcaa
     2941 ttttcacctc tttttgttaa atttattaat gtttgaccat ttctcaattt tcttaatttt
     3001 tcttcagtag caggaaaatt aaaataagca ggaagaagtg gtacagtgtg taatacattt
     3061 cgtgcctgta actctttaat cattgctaca ttatctgctg aaattttacc tgcaaacaat
     3121 ggctttaaac tttcacctct ttttatatat aataaaagat taactagtcc gcgtaaataa
     3181 atcgcatctt ttgtaaggcc tccgctacgg aatgtacggg aagtaatatt aaatgcagtg
     3241 ctgagctcga atccaaaatc cagattaagt tctctgaaaa tttcgataaa ccctgctccg
     3301 ctaactaaat gatgagctgc aactaccctt gctgctaaaa gacgtaatct ggaaacggtt
     3361 aatcctccta ccaaatattc agataataca gcaagtcctt cctgtaattc atcgtatccg
     3421 gaaagaccaa tgtaaagttg tttcagcggt tgcgactttc cgttaagata tgtcaaaaca
     3481 tgcgtcccca cttcatgtgc gatcaaagct tcaactctct caacaggcac tttcagacgt
     3541 tcaccgatta gtaaatttcc ctgcgatacc attaatcctg taatgtcttt cctgatggat
     3601 attttggttt ttatatccgg aagtttttcg cgaaaataat tcagttctat ttcagctctc
     3661 ttagcaaaat cctttgcatt atatttttta cttttaaatc catctcgagc ccgattagat
     3721 aattgttgta atatatcttt tgcaacattc attaatgagt catcaactgt tccataaagt
     3781 tgcatactgc catataaaaa actactgctg cctctgtcac taagcattga aaactttctg
     3841 tccagttcaa gctgctgttc aagaaaaagc tgagctagtg ttggatcttc gatattctca
     3901 attggaatat ggaataaact cctttttgca agtgaaggat ctaatgtaag tggacgataa
     3961 agaaataacg gtactttctc aaaatgattt cgtttgaatt gagaatatgc atagtcaata
     4021 ttatatggag tcacctgtaa tagaaaatca aatcctttat ttatttcggc aagctgcctg
     4081 tccacttccc atactgcctt tcccatactt ctctttccca gtgattgaaa gtttagaggt
     4141 ttaaagggag tgttattttt tgtaaactca aaaaatccat tactcaaagc tcttgaaaaa
     4201 cctttcctga gatttcttaa aagtatcgga tatacttttt cggtttccac attgaagtat
     4261 ataggtttca cttcaattcc cattatatga ataccaagtt tacgtgcttc agaaaatgat
     4321 attaccggag acaattcttt tggagctatt cttggaactg ttattacttc tacttcagat
     4381 ttttctttac gaagttttat atttcttaat gatctttcaa gtgactgaac agtgggtaat
     4441 atttctgtcc cttttgaaat attaatttta aatgaaccct gataagcatt gagttcatta
     4501 tttaggtctt tatcattaga aacccaaact tcaactatta agaaagttcc aaaagattct
     4561 tttaaagttg atgcaatgct tttaataagt tttaacaatt gcttgtggcg tacactggta
     4621 tctttagctg ttaaatatga cgcctctcct gtaaccaatg aatctgataa agaatattta
     4681 tccacttcat tgtatctgta aatacatata aatggaagct gccggtcaat atgaatcctt
     4741 ccccattccg gcaatgatct gcgaactctt ttattctttg ctaatcttga acagacttga
     4801 tttataaatt catctgaaat tgtatttatt acaggcttta tcatttatct gatatatttc
     4861 aattcttcta atattccggg tactgttgac tgtaaagcca taccaatttt ttcaatttga
     4921 cacacatcag gttttccagt ccattcgtcc atgaaaaatt tcttaaactc tattgaaatg
     4981 gaacaggcag attcaggaaa tgtctgatga gtccactttg gaaattgccc tcctttgaat
     5041 tttatatttt cccttacatc aagctttctt ccgtcgaaat caaaattatg cagatctgaa
     5101 ataaaacggt caatcaattt tccccattta tttctgttaa tagttccggt tccaatattc
     5161 acttccggat ttaatttcgg atcagcatgc tctccgtcag ttccgtttct catatggttg
     5221 tatgaatgaa gatcaagtac tgcaaaatgt ccaaattgtt tttcgagagt gctgaatatt
     5281 tcataaactt ctttataaaa taaatcatat tgtgaaaggg aatcggatac aatattctca
     5341 ggtaaatctc ttttccagat atctaatccc caggcgtctt caggtttttg ataaacagct
     5401 ttttcacggg gacggtttag atcaacttca aaccttgacc gtgaaacaat tatttttgta
     5461 tcaccaactg aagtccattc cccggtataa ggatcttctt ctctcaatct atcatcactg
     5521 cttagtttca ttatttcaga aacatcatcc cgcaactctc ttccgtcatg aattgctgcc
     5581 gctacaacag gacctgcgct aaaggttaaa ttccaaaatt tattttgatt catgttttac
     5641 atctttgctg tcagaactca ataatatcca acaagtcttt gattttttta aaattatgtt
     5701 caccaatata attactttta tatgaataaa ttattgattt aattgttctt gatttatttt
     5761 tccgactgaa tgttgatttg aaaaattatc tgaaagtcat gaaaaatttc tttgcctctt
     5821 gggttgcaag gctaaaagga agttaatcat ttttgttgta ggatctttga atcttagaga
     5881 ctttgtggca attgtatcgt tttcagaaaa gctgttttaa atcttaaaga cattggaatt
     5941 tgtaaattgt aattcgtaat ttgaaattac ttaatttaaa atcaaaagtt taaaatgaaa
     6001 atagaatcta tgaactggtc agacgttgaa aattatttaa agaataatga caaatgtatt
     6061 ttgcctgtag gctcgacaga gcagcatgca tatctgagct tatcgacaga caatatactc
     6121 gctgaaaaga tctctgtcga tgcagcagct ccgctgaata ttcctgtctt ccctgtgata
     6181 aattatggtc acactccatt attcatggca tatccgggaa ctgtctccat aagcatagaa
     6241 actttaagtc tgcttttgaa agatattttg aattcacttt cgactcaggg attcaaaaaa
     6301 attctaattg taaatggaca cggcggtaat atgcctttgg aaaagcatgt aaacgaatgg
     6361 tcggctgaac atgaaaatgt aaaagtaaaa tttcataact ggtggaaagc tccaaaggtc
     6421 tgggatttta ttcaacagac agatccggaa tcttcacatg cttcatggat ggaaaatttc
     6481 gaatggacaa gattgaaaga tgtgaaactt cctgatcaca aaaaacccat ggtaaatttt
     6541 gagcttttaa aaaatgcatc accaaaagaa gctcgtgaaa ttttagaaga aggaaactat
     6601 ggcggttact ttaataaaga tgatgcaata ctgaatgagc tgtgggatgt agcagtaggg
     6661 gagacgcggg aattactaaa tgagtggtga gaaatacaga tctattatga aatgtttaaa
     6721 cattttttta tttcagaagc agatccagaa aggtatgaat aaatttatgt ttagaaaatt
     6781 ctacaaacat ttttctaaat aaaatcttca tacctatcca cttttagatt tccagacttt
     6841 ttcaaacacc atcagctgta caaaataaag taactcgaaa ctcgtaactt tctctaagga
     6901 attaccttcc caataaaatt aaatgaatta ttatccacta aagctgcgtc acttgcatct
     6961 gtaaaattat ctccgttcat atcagtattt acatagccgg ctgtaaaatt aaacagatca
     7021 ttatctacaa gtgactgatc agtaatatca actaatccgt tctgatctgc atcaccgtta
     7081 tatatcccgt atctgatatt atttggattt acaagtatct gattatctcc gtatgcctgc
     7141 tgagggaatg ttgtaaaatt atatgattct gtcggatcgt caaaattaat tgtccccgag
     7201 ctccaggtct caattgaatt cctgtgtctg agctgaacat aatattcatt gtcaggaaat
     7261 gcatttggaa aagtcatcgt cgctccgccg gtattactga tatatacttt tgcagaatca
     7321 acttttgaat atggagaaac agaatttctg agatatacat ttacagtatc tctcacagta
     7381 ctttctgtgc ccggattata aaatccctgt atgaacatag taagattcag ttcattatca
     7441 ggatcatctc ctgatacatc tcttacccaa aatgatcttc cgtaagaaga agctatcaca
     7501 tatctctttc cgttttcaag agtactgtca atccatttca tttctgtaat aatagttgca
     7561 tccggcattc cgttattcca tctatgccag gtcacacctg cattggttgt cctgtaacag
     7621 cccatttcag ttccaagata cagataatta ttaagaagtc tgtgcggaac taatccgccc
     7681 aaaggcacat ccggaagatt tccgctgatg ttcgtccagt ttactccttt attggttgtc
     7741 ttaaaaactt tattgccggc agagaaacca ttgatcaatg catatgcagt agtagtattt
     7801 gtaagatggg ggatgacttg tctgattctt aaatttgaag gaattccact ggttctttca
     7861 aaaaaatttc caccgtcata tactcttaac ctttgtcctg tagtaaatga atttaatgtt
     7921 gcataaatta ttgctttagg tgctacgtat tttgagactc ctatattggt cactgaaacc
     7981 gggaatgcac tgacattaag tttattccat gaattacctg catttgtaga ttcataaaca
     8041 tacgcgccgc cgtttgtata aagctttacc ggatttgttc tgtcactgcg cattttggtg
     8101 taccaggtag atgtcggatc aattccggta tgatcttctg tccaggtaaa accttgatca
     8161 gaagatctgt atatttcgaa agaatgatct cctgaataat atcctgcagt tacattaaca
     8221 tataatgaac tgattggatc aatagaaact cctcctccgt caccgccctt tgtaaaattc
     8281 catgtagttc cgccgtttgt agttcctgtg attccattat cctgtgaacc gccgtaaata
     8341 actcctctgt tactttcacc tacatcaaaa ttaacatact gtgtaatcgg aatataattt
     8401 attcctgaag aaaatgtgac tccctgatca tttgaataaa ttacacctcc gtcatttcca
     8461 atataaacat tctgtccgtc tgaagtccat tctattgcat gctgatcagc atgaatatca
     8521 tcatcagtca attctgtcca tgtagttccg ctgttcgcac ttctccagag ccttactcct
     8581 cctgccaaaa ctatattcga attagttgga cagacttcca aaacattatt ataccagccc
     8641 tgatttttaa aaatatttgt acccggtgat acatcagacc aggtagcacc gtcatttgct
     8701 gacatataaa ttcctttaag agtgtcacct gcctgagaat ctatcatgac ataaatttta
     8761 tttgagttgc tcgttccgac tgaaagtgaa gttctgccaa tatcagaagt cggtattccg
     8821 ggtgtagtaa cttttgccca gttcacacca ttgtttgttg atttataaat tccgtcaccg
     8881 catcttgccg catatattgt gttcggactg ttcaggttta ttgcaatatc acttatacct
     8941 acactattgg ccgggcttaa atgtctgatc cagttcagac ctccgtcagt agatctgtaa
     9001 tatccttcag tactggctgc atgtacaata tgagcaaaaa aggactgatg acgtatttta
     9061 taaaatcttc ccgggtcagg ggaaatattt atatgagacc aggtggaacc ggaatttgta
     9121 gttctccaca ttcctgtacc gctcctttga cttggctctc cggttccgat cagaatggtg
     9181 gatggagatc cgggatatga agctaccgaa ccaattgcaa gactggtaat atcttcgcta
     9241 attcctaccg ggagaaatcc cagatatccc cagagaccgc cggaggctgt tgcaagtaat
     9301 ggatttgcag tattcccaac ttctatatcg attatccttc cgctgaatct tgcaccgtt
//
LOCUS       DAORVQ010000127         9346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_3990_length_9346_cov_5.695835, whole genome shotgun sequence.
ACCESSION   DAORVQ010000127 DAORVQ010000000
VERSION     DAORVQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3990_length_9346_cov_5.695835"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            842..3112
                     /locus_tag="PLX80_10190"
     CDS             842..3112
                     /locus_tag="PLX80_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21199.1"
                     /translation="MFKRSILILVLFLSLKSISFSQTDKWENFTDFKSITSIAYDSVQ
                     NIIYCGSRGGIYTVNNSNGTILAKYTNLNGLVNNEILSLAIDNNRKLWVGASDGSISI
                     LDIENQSWKYIFDIKNSNESNKFINYMYLSGNFMFVATGYGIQKISTSSFNFIDAPYY
                     KLGGFPINTPVFSLTLLNNVLYAASASGIAYGNLSSNLNNPSSWLNYSSDNMSNDVKT
                     IESFDNKIFAGSILGYSYFNGTNWLPYPNAVVSNNATNFIKSVGDLLYFISGTTIYSA
                     NKNDLTTITPFLTPNSYSVLSADNNNKLIAGLSNNGIDLSVNNVYTLTFPNGPFTNVF
                     SQITIDENNDIWVAGGLGNNGFYSFDGVTWENYNTASHPEIGSSNWFQKISSGNGKVW
                     AYGFGGGPTEVFGNTITNFNTLNTNLPGISNNPTFCSSYGGSYDNNQVMWVTFFATNS
                     GSSLYAYQGSNQWISFINPSFIGNASLSQTVVDSYNTKWIVMSGARSGVYFFNENGTI
                     NNPSDDVFGFYDNANFGSEVTNVNDIIVDKNNEIWIATNNGIFIISNPFGAIQNPNNK
                     PAPQKLGIISGNLKVPFTENCLSLTSDILNEKWIGTDNNGVFNLSSDGSTLQGNFKTT
                     NSPILSNKISTIAVSNKTGIAYFGTQNGMSSYKTNAIEPVQDFDEITVSPNPFVIPSA
                     ENLQIDGLIENSTIKIITLSSDVVVEFDSPGGRIASWNGYNSQNQLTPTGIYIIVAFN
                     QDGSKVGTGKVAIVRK"
     gene            3163..3927
                     /locus_tag="PLX80_10195"
     CDS             3163..3927
                     /locus_tag="PLX80_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HQY21200.1"
                     /translation="MLVIPVIDIKDGKCVRTVQDLSENTEFYSESPIIMAKLFRKENF
                     KCIHITDLNGALFGDMKNFELIKEITDSVDIPVQLGGGIRDMKTANRIINELGIYRIV
                     IGTAALTDPEFIKDAIKEFTHNKIVIGIDEKLNNVVKDGWINYADITPLEFAIQMEDA
                     GVKRIIYQDVTRVGNLSGPHIERLLEIANNTDLKITSAGGISNYLDLKKVMSIEHLAI
                     DSVMISRALYENQFPCQMIWRDLESDDTSLELPNVK"
     gene            complement(3932..5110)
                     /locus_tag="PLX80_10200"
     CDS             complement(3932..5110)
                     /locus_tag="PLX80_10200"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQY21201.1"
                     /translation="MKKNISLLLTIILFLNINMLSCKSTDNKLETNGDQKNTTLNSDI
                     QDSGIYKAEVFTEGLYVPWSIVFTSPERMLVTERNGRIRIINNGTLEANPLKSFDDVS
                     SRGEEGLMGLTLDPNYENNKFIYVSYAYDSGDELEVKVIRYKDKGDELADEKILMNNI
                     PAERYHAGCRLRFGPDGKLYVTTGDAGERELAQDVKNLYGKILRVNSDGSIPEDNPYP
                     GNPVWSYGHRNPQGIDWYPGTDILYSTEHGPSGFDGPGGGDEVNVIVKGGNYGWPVVS
                     HKESEEGMISPLLIFTPAVAPASGMFYDSDVIPEFKNNFFFGCLRGNTIYRIIPEESD
                     PSRMKSFEKINGIDFTKIGRIRDVAQGPDGAIYFSSSNKDGRGDVRNGDDKIYRIVKN
                     "
     gene            complement(5197..6489)
                     /gene="hutI"
                     /locus_tag="PLX80_10205"
     CDS             complement(5197..6489)
                     /gene="hutI"
                     /locus_tag="PLX80_10205"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQY21202.1"
                     /translation="MNILIKDITQIVTCRNKGKLPKSGKNQSDIGLVKNGNIFIEKGK
                     FAFIGSNAELKKFLAGNTAKKYEIIDGSGKVITPGFIDSHTHFVFAGSRENEYEMRQA
                     GKSYQDIAKAGGGILSTVKAVRNTNKKELKEISERRLGKFIEYGTTTIEGKSGYGLDL
                     GNELKLLEVMKDLNDDNKYGIDIVSTFLGAHAVHPDKKKADYIYEICHNFISIISKEK
                     LATFIDVFVEENYFTKDEADQIFSFGERYGLIPKLHTDQFNSIGGIEKAVKYKAASVD
                     HLEVLSEDDIELLSTHNKDRKNRSIIATILPGVSYFLGIPYAPARKLISKKIPVALAT
                     DFNPGSCMTENLQTIMSLASIHLKMNAEEILNAVTINAAFALNMEKTIGSIEPGKQAD
                     LLIFDMPSYKYLVYNFGVNNLETVIKKGKFAFQKIDQN"
     gene            complement(6561..7571)
                     /locus_tag="PLX80_10210"
     CDS             complement(6561..7571)
                     /locus_tag="PLX80_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21203.1"
                     /translation="MSKLLEVKNLKTYFFTDDGVAKAVDDVSFSLEKGETLGIVGESG
                     CGKSVTALSIMRLIAEPPGKIVGGEIFFKGKNVINFSEKEMQELRGNDIGMIFQEPMT
                     SLNPVFTCGDQIEEAVILHQKLSKEQAKVKAIEMLNLVGIPAPEQRYNEYPHQLSGGM
                     RQRIMIAMALCCNPEILIADEPTTALDVTVQAQILELIKKLQTDLGMGVIIITHDLGV
                     IAEISDKVSVMYASKVAEFGTADQIFYNSKHPYTIGLLNSIPKLTGSGHGKLATIEGS
                     VPAPTNYPKGCHFCTRCIFAIEKCWNEQPPLVEIEKGHISACWRVSEIDKFKEMLQVP
                     AV"
     gene            7876..8775
                     /locus_tag="PLX80_10215"
     CDS             7876..8775
                     /locus_tag="PLX80_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HQY21204.1"
                     /translation="MRYDNQNLELTGFEYKTGNILSIIFFSILVLTILINLFVWKNPG
                     NLILDFLLAISIIGILCLNFSIFYFQKDLRIILSSIFLVINLILLSSMMSVLFSSKKD
                     FSFFKSIFIFLFIFIGGLSVIFFSIYNYKDDESKYAGGNIKADAEAILGAAVWGGNRP
                     SPVLKDRIDKGFEIYIKGFVPKIIITGGGSPNELTEGEVSKNVLIKYGVPEENLILEN
                     ESSSTIEQIHFIRDSLYHKQNWKKIIIISDNFHLKRAAEICSFNNIKADCISSDKVLS
                     AEGSALFCLKESLALIVFWMVGI"
     gene            9094..9342
                     /locus_tag="PLX80_10220"
     CDS             9094..9342
                     /locus_tag="PLX80_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21205.1"
                     /translation="MEFGIKDLPAKLREIPSPMDTKDFGTALLKSLSYPVLKIPSVLI
                     PDEHNYILNPLHKDSKNFKVISVKNFDYNERIKVTNKN"
BASE COUNT         3023 a         1629 c         1548 g         3146 t
ORIGIN      
        1 ctttggtaaa acagatcagc agaaatacga gtgacccgaa cagttcactg tcgaaatggg
       61 atataaaaaa cgacgatggt aagtatgttg caagcggaat gtatgtagtt tatatagact
      121 gcaaagctgc aggtgcaaag actttgaaga tagcggtgtt tcagagccgg tgacctcaaa
      181 gacttttttg acctttaagg ctaaaaggct cgaagaaact aagatattaa gacattaaga
      241 aaataagaat ctaagataaa aaatattaaa gaacataatt aattttaatg aattgttaca
      301 aaaagttttt tctttgagcc ttcgagcctt tgaggtaatg aagaaaaaga ttttaaatgc
      361 agtcagcatt ttcatatatg aaaggcaact tcgttctttg atcatataaa ttaaaaaaaa
      421 ccaggtcact gttagtctgt cctgaaaata aaaaaaggag ttcctgtcga gggtcggggg
      481 cttctttttt ttgcttacct caatggtccg aagactcaaa gagatttatt tcaaatgatt
      541 ttataattgt gtcttttgta atttggctaa acgatctttt tagtataaat tttaagattg
      601 gaaacgaaat catcttttct aataaatcaa atagttattc tttgagcctt taaaacttcg
      661 gggtatagct ctacagtaaa aaattgaata aaaaaattat attgttttta aaaattaaat
      721 actcaatatt ggaactgatt ttcaaaaagc gctgcatatt tttattaaac cttcgaataa
      781 atattagagt atagcctttt ctacatgttc cgttttctta aaaaatttaa atcataattc
      841 tatgtttaaa aggagtattt taattctcgt tttattttta tcattaaagt ccatttcatt
      901 ctcacagaca gacaaatggg aaaatttcac ggattttaaa tctattacat caattgccta
      961 tgactctgta caaaacatta tttactgcgg atccagagga ggaatttata ctgttaataa
     1021 ttccaatgga actatacttg caaaatatac aaatctgaat ggtctggtaa ataatgaaat
     1081 tttatcttta gctattgata ataacagaaa actatgggtc ggagcatctg acggatcaat
     1141 ttccatatta gatatagaaa atcaaagctg gaagtatatt tttgatataa aaaattctaa
     1201 cgaatccaat aagtttatta actatatgta cttatcaggg aatttcatgt tcgtagctac
     1261 aggttacgga attcagaaaa tttccacctc aagttttaac tttattgatg ctccatatta
     1321 taagctcggt ggatttccaa taaatacacc tgtattttcg ctgacacttt taaataatgt
     1381 actttacgcc gcttcagcct cgggaatagc atatggtaat ttatcatcaa atcttaacaa
     1441 tccgtcctca tggttaaatt acagcagcga taacatgtca aatgatgtaa aaactataga
     1501 atcatttgat aataaaattt ttgcgggttc aatacttggg tacagttatt ttaacggaac
     1561 aaactggctg ccatatccaa atgcagtagt ttcaaataat gctacaaatt ttattaaatc
     1621 tgttggcgat cttctttatt ttatttcagg tacaacaatt tattctgcaa ataaaaatga
     1681 tcttactact ataacaccat ttcttactcc aaattcttat tcagtattat ctgcagataa
     1741 caataataaa ttaattgccg gcttatcaaa taacggaatt gatttatccg taaataatgt
     1801 atatacactt acatttccaa atggtccgtt tacaaatgtt ttcagtcaga tcactataga
     1861 tgaaaataat gatatttggg ttgcaggtgg tcttggaaat aacggctttt attcatttga
     1921 cggagtcaca tgggaaaatt ataataccgc aagtcatccg gagattggtt cgagtaactg
     1981 gtttcaaaaa atttcctcag gaaatggtaa ggtctgggca tacggctttg gaggaggacc
     2041 aactgaagtc ttcgggaata caattacaaa tttcaataca ctgaatacta atcttcccgg
     2101 aatctctaat aaccctactt tctgttcaag ttacggtgga tcatatgata ataaccaggt
     2161 gatgtgggtg acattctttg ctacaaattc cggaagttcc ctgtatgcat atcagggaag
     2221 taatcagtgg ataagtttta taaacccttc gtttattgga aatgcatctc tttcccaaac
     2281 tgtcgtagat tcttataata caaaatggat cgtgatgagc ggagccaggt caggtgttta
     2341 tttctttaat gagaatggta cgattaataa tccttccgat gatgtgtttg gtttttatga
     2401 taatgctaat tttggttcag aagtgacaaa tgttaatgac atcattgttg ataaaaataa
     2461 tgagatctgg attgcaacca ataacggtat ttttattatc agcaatccgt ttggtgctat
     2521 tcaaaatcca aataacaaac ccgctcctca gaaacttgga ataatctcag gaaatcttaa
     2581 agtgcctttt acagaaaact gtttatctct tacaagtgat attctgaatg aaaaatggat
     2641 aggaactgat aacaacggtg tttttaattt atcatcagat ggatcgactt tacagggtaa
     2701 cttcaaaaca acaaacagtc caattctttc aaataagata agtactattg cagttagtaa
     2761 taaaacaggc atagcatatt ttgggacaca aaacggaatg tcatcttata agaccaatgc
     2821 tattgagccg gttcaggatt ttgacgagat tacagtatct ccaaatcctt ttgtaatacc
     2881 ttcagcagaa aatcttcaga tagatggtct gatcgaaaac tcgactataa agataataac
     2941 tctcagcagt gatgtagtag tagaatttga ttcacccggc ggcaggatag catcatggaa
     3001 tggatacaat tctcaaaacc aattaactcc aaccggaatt tatattatag ttgcttttaa
     3061 tcaggacgga agcaaagtag ggactggcaa agtggcgatt gtgagaaaat aattttttgt
     3121 ttgaatgaat ttttttagtg taataaaaaa tagtaattgt aaatgttagt catacccgta
     3181 atagacataa aggacggtaa gtgcgtcagg acagttcagg atctcagtga aaatacagag
     3241 ttctattccg aaagtcctat cataatggca aagcttttca ggaaagaaaa ttttaaatgt
     3301 attcacatta cagatctcaa tggagctttg tttggtgata tgaaaaattt tgaactgata
     3361 aaggagatca cagactcggt ggatattccg gtacagcttg gcggcggaat cagggatatg
     3421 aaaacagcaa acaggatcat taatgaatta ggaatttata gaatagtaat cggcactgca
     3481 gcactgacag atcccgaatt cataaaagat gctattaaag aatttacgca taataaaata
     3541 gtgatcggga ttgatgaaaa actaaacaat gtagtaaagg atggatggat aaattatgct
     3601 gacattacac cgctggaatt tgcaattcaa atggaagatg ccggagtcaa aagaattatt
     3661 tatcaggatg ttacaagagt aggtaatcta tccggaccgc atatagaaag acttctcgag
     3721 atcgccaaca acactgacct taagatcact tccgccggag gtatttcaaa ttatttagat
     3781 ctgaaaaaag tgatgagcat tgagcatctg gcaatagatt ctgtcatgat ctccagagca
     3841 ctttatgaaa atcaatttcc ctgtcagatg atctggagag atctggagtc agatgacact
     3901 tcactcgaac tccctaatgt aaaataattt tttaattctt tacgattctg taaatcttat
     3961 catctccgtt tcttacatct cctcttccat ctttattact ggatgaaaaa taaatagcac
     4021 cgtcaggacc ctgagctaca tcccttattc ttccgatctt agtaaaatca attccgttaa
     4081 ttttttcaaa agatttcatt cttgaaggat cactttcttc aggaatgatc ctgtatattg
     4141 tatttcccct tagacaacca aaaaagaaat tgtttttaaa ttcaggaatg acatcggagt
     4201 catagaacat tcctgatgcg ggagcaacag caggtgtaaa aatcagcaga ggagagatca
     4261 ttccttcttc gctttcctta tgagagacga caggccagcc gtagttaccg cctttgacaa
     4321 ttacattaac ttcatcacct ccgccgggtc cgtcaaagcc cgagggaccg tgttcggtag
     4381 aatacaaaat atctgttccc ggataccagt cgattccctg gggatttctg tgtccatagc
     4441 tccagactgg atttcccgga tatggattat cttcaggaat gctgccatct gaatttaccc
     4501 gtagtatttt cccgtaaaga tttttaacat cctgtgcaag ttctctttct cctgcatcac
     4561 cggttgtaac atataatttt ccgtcaggtc caaatctcag tctgcatcct gcatgatatc
     4621 tctctgcagg aatattgttc attaaaattt tctcatcagc aagttcatca cctttgtctt
     4681 tatatctgat gactttgact tcaagctcgt caccgctgtc gtatgcatag ctgacatata
     4741 tgaatttatt attttcataa ttgggatcca gtgtaagacc catcagtcct tcttcgcctc
     4801 tcgaagaaac atcatcaaaa gattttagag gattggcttc aagagttccg ttattaatta
     4861 ttcggatccg cccgttcctt tcagtaacga gcattctttc aggtgaagta aatacaatac
     4921 tccaaggaac ataaagtcct tccgtaaata cttcagcttt gtaaattcct gaatcctgaa
     4981 tatcagaatt taatgttgta tttttttgat ctccgtttgt ctccagctta ttatcagttg
     5041 atttgcagga gagcatatta atattcagaa acaaaattat tgtcagaagt aaagaaatat
     5101 tttttttcat gattaatttt agataataaa attactatta agatgttccg actaaggtta
     5161 tatttattac tgttgcctta ttttatgcaa tgttgtttaa ttttgatcaa ttttctgaaa
     5221 agcaaattta ccttttttta ttacagtctc aagattattc acaccgaagt tatagacaag
     5281 atatttatac gaaggcatat caaagatcag cagatcggct tgtttgcccg gttcgatact
     5341 gcctatggtt ttttccatat tgagcgcgaa agccgcattg attgtaacag cattaagaat
     5401 ttcttcggcg ttcatcttca gatgaataga cgcaagcgac attatagtct gtaaattttc
     5461 cgtcatacag cttcccggat tgaaatctgt tgcaagtgcc actggaatct tctttgagat
     5521 cagctttctc gccggggcgt aaggtattcc aagaaaatat gaaactcccg gaagtatggt
     5581 cgcaattatg cttcggtttt ttctgtcttt attatgtgta gagagtaatt caatgtcatc
     5641 ttcagacaat acttcaagat gatctactga cgcagcttta tattttactg ctttttcaat
     5701 tcctccgatg gaattaaact ggtcagtgtg taattttgga atcaatccgt accgctcacc
     5761 aaaagaaaat atctgatcag cttcatcttt cgtgaaataa ttttcctcta caaacacatc
     5821 aataaaagtc gcaagttttt cttttgaaat tattgaaatg aaattgtgac atatctcata
     5881 aatataatcc gccttctttt tatcaggatg gacagcatgc gctccgagga aagttgatac
     5941 tatgtcgatt ccatatttat tatcatcatt aagatctttc atgacctcga gcaacttcaa
     6001 ttcattgcca aggtcaagcc catagccgga ttttccttcg atcgtagtag taccatactc
     6061 aataaattta ccaagtctcc tttctgaaat ttcttttaac tctttcttat tggtatttct
     6121 cacagcctta acagtcgata atattcctcc tcctgctttt gcaatatcct gatacgattt
     6181 ccctgcctgc ctcatttcat attcattttc tcttgagcct gcaaatacaa aatgcgtatg
     6241 tgagtcaatg aatccgggag taattacctt cccacttccg tctattattt catacttttt
     6301 agcagtgtta cctgccaaaa attttttaag ctctgcatta cttccgataa aagcgaactt
     6361 tcctttctcg ataaaaatat ttccattttt cacaagacca atgtcagatt gattttttcc
     6421 tgactttggt aatttacctt tattccggca agtaactatc tgcgtaatat cttttattag
     6481 tatattcata atttaaaaaa agaaaaccct aaacttattt aataaaaaaa ttcagggttg
     6541 taaaaaaatt atttgtaaaa ttaaactgcg ggaacctgta gcatttcttt gaatttatca
     6601 atctcgctga ctctccagca agctgatata tgaccttttt ctatctcaac cagtggcggc
     6661 tgttcgttcc agcatttctc aattgcaaat atacatcttg tacaaaaatg acaacccttt
     6721 ggataatttg tcggcgcagg tacgctgcct tcaatcgttg caagctttcc atgacctgaa
     6781 ccggttaact ttggtattga attcaaaagt ccgattgtat aaggatgctt actgttgtaa
     6841 aatatctgat ctgcagttcc aaattcagca actttagaag catacataac tgaaacctta
     6901 tctgaaattt ctgctattac tccaagatca tgagtaataa ttattacacc catcccgaga
     6961 tcggtctgaa gtttttttat cagctcaaga atctgtgcct gcacagtaac gtcaagtgca
     7021 gtagtcggtt cgtcagctat cagaatttcc ggattacagc aaagtgccat agcgatcatg
     7081 atcctctgcc tcatacctcc cgaaagctga tgtggatatt cgttatatct ctgctcaggt
     7141 gcaggaatac ctacgagatt aagcatctcg attgctttta ccttagcctg ttcttttgat
     7201 aatttctgat gaagtatcac agcttcttct atttgatcac cacatgtaaa gacaggattt
     7261 agagaagtca ttggttcctg aaatatcatt ccaatatcat ttcctctaag ctcctgcatt
     7321 tctttttcag aaaaattaat gacattcttt cctttaaaaa agatctctcc tccgactatc
     7381 tttccgggag gctcagcgat cagtctcata attgataaag cagtaacgct ttttccgcaa
     7441 ccggactcac ctacgattcc cagagtttca cctttttcaa gtgaaaagct gacatcatca
     7501 acggctttag ccacaccgtc atctgtaaaa aaataagttt ttagattttt aacttcgaga
     7561 agttttgaca ttaattagct ccgtatttaa tgttttcaag ttactttaaa tatggaattg
     7621 tttcaagtaa attattaaaa tcacttaaag atagataata gttaatagtt aatagttaat
     7681 aattttatgt tgcaattttc ttttaacctt tttttaactg aacaaattaa tttactttaa
     7741 aagtattata aattaagtta agtttgtcaa aggaaattat ccttattaat taaactcact
     7801 cagatcaaaa aaggcagatt taaattattt ctgattttta ttctcagcat catttcattc
     7861 ttttatcttt attttttgag atacgataat cagaatttgg aattgacagg ctttgaatat
     7921 aaaacaggca atatactttc catcatattt ttttcaatac ttgtattaac gattttgata
     7981 aatttatttg tgtggaaaaa tccggggaat ctgatattgg attttttact ggcaatttct
     8041 ataattggaa ttttatgtct gaatttttca attttttatt ttcaaaaaga cctgaggatc
     8101 attttatcta gtatctttct tgtgataaac ttgatattac tttcgtctat gatgagtgtt
     8161 ttattttcca gtaagaaaga cttcagtttt ttcaaatcaa tatttatctt tttattcatt
     8221 tttatcggcg gattgtctgt gatatttttt tcaatttata attataagga tgatgaatcg
     8281 aaatatgccg gcggaaacat aaaagctgat gccgaggcaa ttctcggtgc agccgtttgg
     8341 ggagggaaca gaccctcacc ggtgctcaaa gacagaattg ataaaggatt tgaaatttat
     8401 atcaagggct ttgttccaaa gataattata accggcggcg gctcaccaaa tgaactcacc
     8461 gaaggtgaag tctcaaaaaa tgtattgata aaatacggcg tgcctgagga aaatcttatt
     8521 ctcgaaaatg aatctagctc tactatagaa caaatacatt tcatccgcga cagcctctat
     8581 cataaacaaa attggaaaaa aattataatt atttccgata atttccatct caaacgcgca
     8641 gcggagatct gcagcttcaa taacataaag gcagattgca tctcttctga caaagtacta
     8701 tctgcagaag gatcagctct tttttgtctc aaagaaagtc tggctctgat tgtattctgg
     8761 atggtgggaa tctaatcaat taaaaattac aaattacaaa ttacaaatta caaattacaa
     8821 ataattaatc attaaaaaaa tattagtaat ttaaatcctt aaaaaaaaaa ttaattatcc
     8881 cgatgtaaaa aattttcaca taagttactt tacctgagtt ttattcattg agttaaatga
     8941 gattttggat ggtggaatca catctctgtt ccgtatgtgt attcttctta aagtagagca
     9001 cgtgcagtac ttgagtttac agtaaatgta gatttatatt tcattccagg ttcactttct
     9061 attgtaactg ttcagatacc tgataaagat attttggaat ttggcattaa agacttacct
     9121 gctaaactga gagaaattcc ttcaccaatg gatactaaag attttggaac agcattatta
     9181 aaaagtttat cttatcctgt tttaaaaatt ccgtcagtac taattcctga tgagcataac
     9241 tatattctta atccacttca taaagatagt aaaaacttta aggtaattag tgttaagaat
     9301 tttgattata atgaaaggat caaggttact aacaaaaatt agaata
//
LOCUS       DAORVQ010000128         9292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4030_length_9292_cov_5.283209, whole genome shotgun sequence.
ACCESSION   DAORVQ010000128 DAORVQ010000000
VERSION     DAORVQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_9292_cov_5.283209"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLX80_10225"
     CDS             complement(<1..1008)
                     /locus_tag="PLX80_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HQY21206.1"
                     /translation="MSNNKSITEEAADFVNELFKNKLQTIYVYHNITHTEEVVEAAKK
                     IGNKLNLNDEDLEIVTLAAWFHDTGFIERVNNHEDVSIDIAVKFLTEKNYKQENIEMI
                     KGCINATRYPQSPTNILEEILCDADLYHLGNKNFDFKNDLLRIEWEKSEGKIYSDIEW
                     IKINLDFLTTHKFYTKPARKSLTENKIDMLVKLQKKHRKKIAESEENVKSDLKIDIEK
                     QKIESKKESSSKADRGIETMFRNVIRTHVEFSAMADNKANIMISVNTLIIGGLITFLL
                     RKLDTNPQLIIPTFSLLLVSLTCIVFGVLVTTPKNTAGVFSKDDIVNKKTNLLFFGNF
                     FK"
     gene            complement(1174..1602)
                     /locus_tag="PLX80_10230"
     CDS             complement(1174..1602)
                     /locus_tag="PLX80_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013188390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY21207.1"
                     /translation="MTILMPFHLALPVKELGETADFYKNVLNCKQGRRSEKWMDLDFF
                     GHQLVLHITKDKKSEDIDPTNPVDGHDVPIPHFGVVLNYDDWYEMEKKLKEYGMKFLI
                     EPYIRFKGEAGEQATMFFYDPSGNAVELKAFKDLSQLFAG"
     gene            complement(1635..4838)
                     /locus_tag="PLX80_10235"
     CDS             complement(1635..4838)
                     /locus_tag="PLX80_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY21208.1"
                     /translation="MSVSKLAVDNKVTVYILMVIIIIVGLSSYNSIPKESAPSIIIPN
                     IFVSTLYFGVSPQDMESLVTQEIEKEVKGISDVKKITSISQESFSMVNIEFNPDVIID
                     DALQKVRDKVAIAKTKMPQDIEEPSINEINLSEQPMLYINLSGNLGLARLKDIADNIS
                     DDIEGIPGVLSADVKGGLEREVKVNVDADRLKYYNLTLNDITTKIGNENLSIPGGSVD
                     IGSQNYLVRVPGEYEDPELIRDLIIKSDNGKPIYLRDVANVIYDYKERTTLARENGNE
                     AVTIVIKKRSGENIISIADEIKSLVKEKESTFPEGLKVSFTGDESISIKNTVHELENG
                     IITGFLLVCLILLAAMGFKNSLLVASSIPFSFLISFIVLAGLGITMNIVVLFGLILVL
                     GIIVDDAIVVTENIYRLQETEGYNPHDAAIEGPREVQVPVTIATFTIISSFAPLLFFP
                     GIVGEFMKYLPITLIVCLFSSLFVAMIINPVLSAKYINFKEDRAKHESRLKWYNIINK
                     FHLWFDKLFAKIVKNYEKVLRWCMGRRKLTIAMTVVLLIFVFIIYGMFSKGVEFFPKV
                     EPQQAFIYVNMPVGTNLDKSNEVTKVVEKKLPGFKDIEYFLTNVGSEIGEGFGSDIAN
                     KNTITLNFYEKADRSQNSFKTIEEIRDSITNITTADVRIQMQTGGPPTGPPINLEISG
                     DDFAKLGEISDEIKRQIVNIDGIKDLKDDFDEARPEIKISIDREKASLYGMNTAIIAS
                     DIRTAINGTAASKFRVSDKEYDITVRLDSSQREDILSIENIFLPGKDGAKIPLTSVAQ
                     VEFSGGIGAINRKDLKRVVTVSANAEGRLGNDILTDVRAKLKDYKLPDGYSLTYTGEQ
                     EDQQESSDFLSKAFLVSLLLVFFFLVIEFNSIMSPIIIMFTVILSLIGVLIGLLVTQT
                     PFGIIMTGIGVISLAGIVVRNAIILLDFQAELERRGMNREESVIQSGMIRLRPVFLTA
                     AATILGLIPLTTGVDFDWRSFSWLLDGQNTAFWRPMGVAIIFGLAFATFLTLVIVPVM
                     YVSVNNLLDRFKKKNPEEVSGSISGNDTESDILLTTK"
     gene            complement(4861..5904)
                     /locus_tag="PLX80_10240"
     CDS             complement(4861..5904)
                     /locus_tag="PLX80_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY21209.1"
                     /translation="MKNIIYIMLLFFTLSFGMIISSCGDDGETKTENEKTLQAVKVSE
                     IVTSEFLETYKVVGTVKPFETAKISAEEGGLITYMPFDKGSRVSRGQTLVKLKKDIDF
                     ASYEQAETQYELAKSNYERIERLYYESVSTEQDFTNAKFQLELAEKSLNLIETRLSKS
                     YITSPIYGIVDQKFMSRGEVCATGAPILNIVNISKVKISAGIPERFVEEVKKGSLVKI
                     TFDIYPGEEFTGSVNYVSPTLSEKNRTFEIEMVIPNKDGKLKPEMSANILIEKSMITE
                     AIVLPQDLIIDFGNDKFVYVLENGTARKKIITLGGRSNNKVHIISGLNSGDVLISEGY
                     QSVSDGDKVEIIN"
     gene            complement(5929..7257)
                     /locus_tag="PLX80_10245"
     CDS             complement(5929..7257)
                     /locus_tag="PLX80_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY21210.1"
                     /translation="MTKTHFKKLLILLFCVLVVGVEPVFSQQKLIITSIEQAVNLALR
                     DNSDVINARFERLKAEKIVSEVYSENLLPSLTMDTRLSRSFKKQVLNLFGETFEVGSD
                     NNFNTGIDISEPIPILGTPVFSGIRIAEYYSKIQSENVELKENEVANTVKNNYFSVLL
                     SKAIVDVNKITYDNAEANFRVVESRYRNGVATEFDFLRARVKVENVRPILLKSERDFD
                     ISKKLFSNAIGIKVNDNIEIIDSLTYDSTEIWESTDQLIKRISENYVTVRQLRLNKKV
                     NEELVNVQQAYYLPKLFLYGRYELISQENDNVPISKYRFYNTFNAGIGLTWDLNLFRN
                     SYKVDQAKLDVKISQESLIDVQQKLKLRSEAALISLEDAKQRILSTRETVTLAERGLD
                     LANNSYREGVLNQIDVQDAELTLYNSRLGYYQAVYDYHIAKAELEKLLEK"
     gene            complement(7264..7905)
                     /locus_tag="PLX80_10250"
     CDS             complement(7264..7905)
                     /locus_tag="PLX80_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY21211.1"
                     /translation="MLKISKQIKEREKILIFCTHKFHKEGFYKTSMDEVAKEMCISKK
                     TIYKHFPSKENLLEEICDHTTKVIYNNVDDIVEGEGNVVLKFIMLLNMYSNFTINISD
                     KWLNDLKVHHPHILKNINERRTDKINGILSKLIKQGKKEKLIENYPATIIISTFIHSI
                     SGVMQPEFLINNKFTIQNAFRITYEMLLNGILTQLGKNIFNKEKNLFAKEIKL"
     gene            complement(8155..9177)
                     /locus_tag="PLX80_10255"
     CDS             complement(8155..9177)
                     /locus_tag="PLX80_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21212.1"
                     /translation="MKQSNSYRDRKINSSDNFNKSDDMFENLRNENTENSFPEVEKWL
                     RKVDSQKTFKISSNHERKFFNMKSPKIKLAYAFLILAFVVTACNYPVTQQEPVADVIK
                     WTVDQNDTKTISRLSSLEWTKNSAIKISTADNGNSIAEYTLIVSKEDHANSKKYLEQL
                     NTMEGILTVNLVPLNEQITRPVYSLALIQIFKIDINATNMSDEELRNQISSQLEQNGV
                     SGVQISFERNNDGRRISKVIIPEEEIKNKKGFDLTIQDGDENNKTMEVRKFRNKADGE
                     VDRFEGKSDEEIKKMVVEDLKDKNINPEDINVIRKDGKIMIKISKKNDGTETEDEFQF
                     EGEVGK"
BASE COUNT         2794 a         1713 c         1262 g         3523 t
ORIGIN      
        1 tttgaagaaa tttccaaaaa aaagtaaatt agtttttttg tttactatgt catcttttga
       61 aaaaacacca gcagtatttt ttggtgtagt aactaagact ccaaatacaa tacatgtcag
      121 actgactaaa agaagagaga aagtcgggat aatcagttgt ggatttgtat caagttttct
      181 taaaagaaaa gtaataagac ctccaataat aagagtatta acactgatca taatatttgc
      241 tttgttgtct gccatagcac taaattccac atgagttctg atgacattcc gaaacatagt
      301 ctctatgcct ctgtctgctt tagaactgga ttcttttttt gattctattt tttgtttctc
      361 aatatcgatt ttaagatcac ttttaacatt ttcttctgat tcggcaattt tttttcgatg
      421 ttttttttgc agctttacaa gcatatcaat tttattttcg gtaagtgatt ttcttgcagg
      481 ctttgtataa aacttatgag tagtaaggaa atcaagattg attttaatcc attctatatc
      541 tgaataaatt ttaccttcag atttttccca ttctattctg agcagatcat tcttgaaatc
      601 aaagttttta tttccaaggt gataaagatc agcatcacat aaaatttctt caagaatatt
      661 agtaggtgac tgaggatatc ttgtagcatt aatgcagcct tttatcattt caatattttc
      721 ttgtttgtaa tttttttcgg taagaaattt tacagcgata tcaatactaa catcttcatg
      781 attattaacc ctttcaataa aaccggtatc atggaaccat gcagcaaggg taactatttc
      841 aaggtcttca tcattgaggt tcagtttatt tcctattttt ttagctgctt caactacttc
      901 ttcggtatgt gtaatgttat ggtaaacgta aatggtctgg agtttattct tgaaaagttc
      961 atttacaaaa tcagcagctt cttcagtaat acttttattg tttgacatta ggaggattat
     1021 taaataatga aataaaaata tgaattttta ggtagataat aaacagtatt aatttaatat
     1081 ggtcaaattg aagttatatg gaataaacgg tttaaaccgt ttatttaatc cagaagtaaa
     1141 aaaacggttt aaaccgtttt tccccataat aaatcaaccc gcaaacagct gcgacaaatc
     1201 cttaaatgcc ttcaactcaa ctgcattccc ggaaggatca taaaaaaaca ttgtcgcctg
     1261 ctcaccggct tctcctttga acctgatata aggttctatt aaaaatttca ttccgtattc
     1321 tttcaatttc ttttccattt cataccagtc atcataattt aatactacac caaaatgcgg
     1381 gatcggaaca tcatgaccat caacaggatt tgttggatca atatcctctg attttttatc
     1441 cttcgtaata tgcaatacca actgatgacc aaagaaatcc agatccatcc acttctcact
     1501 tcttcttccc tgtttacaat ttaaaacatt cttataaaaa tctgcagttt cacccagttc
     1561 tttgaccggt aatgcaagat gaaacggcat aagtatagtc atttttaaaa aattatttta
     1621 ttaattaact taaactattt agtagttaac aaaatatctg actcagtgtc attaccggaa
     1681 atacttcccg atacttcttc tggatttttc tttttaaatc tgtccagtaa attatttact
     1741 gatacataca taaccggtac tataaccaaa gttaaaaagg ttgcaaatgc taatccaaaa
     1801 ataattgcta ctcccattgg tctccagaat gctgtgttct gaccgtccaa caaccaggaa
     1861 aaacttctcc agtcaaagtc tacaccagtc gttagcggta taagtccaag tatagttgca
     1921 gcagcagtaa ggaataccgg acgtaatctt atcattcctg attgtatcac agattcttct
     1981 ctgttcattc cacgtctttc taactctgcc tgaaaatcta aaagtatgat cgcatttctg
     2041 acaacaattc ctgccagtga gatcactccg atccctgtca ttataattcc aaatggagtc
     2101 tgagtaacca acagtccaat taatacacca attaaagata aaataactgt gaacataata
     2161 attatcggag acataataga attgaattct attaccagaa agaaaaatac taaaagcaaa
     2221 gaaactaaaa atgctttact taaaaagtct gatgattcct gctgatcttc ttgttctcca
     2281 gtatatgtta atgaataacc gtcaggtaat ttgtaatctt tcagctttgc tcttacatca
     2341 gttaatatat cattgcccag tctgccttca gcatttgcag atacagttac tactcttttc
     2401 agatcttttc tgttgatagc tccgatacct ccggaaaatt caacctgtgc aactgaagtt
     2461 aaaggaattt tcgctccgtc ttttccggga agaaaaatat tttctattga taatatatct
     2521 tctctctgac tcgaatctaa tctgactgta atgtcatatt ctttatcaga tacgcggaat
     2581 ttacttgctg ctgttccgtt gattgctgtt ctgatatcag atgcaattat tgcggtattc
     2641 attccataca aagatgcttt ctccctgtca attgaaattt tgatctcagg tcttgcctca
     2701 tcaaagtcat ccttcagatc cttgattccg tcgatattta ctatttgtct ttttatctca
     2761 tctgaaattt ctcctaactt tgcaaagtca tctcccgaaa tttccaggtt tatcggtggt
     2821 ccggttggag gtccgcccgt ttgcatttgt atccttacat cagccgtagt aatatttgta
     2881 attgaatccc tgatctcttc aattgtttta aatgaattct gagacctgtc tgccttttca
     2941 taaaaattta aagttattgt gtttttattt gcaatgtcac ttccaaatcc ttctccaatt
     3001 tcagacccga catttgttaa aaaatattca atatctttaa atccgggaag ttttttctca
     3061 actactttag tcacttcatt ggatttatct aagtttgtac ctaccggcat gtttacataa
     3121 ataaatgcct gctgcggttc tacctttgga aaaaattcaa ctcctttact aaacattcca
     3181 taaataataa atacaaaaat caaaagaact actgtcattg caatcgttaa ttttcttctg
     3241 cccatacacc agcgtaatac cttttcatag tttttcacaa ttttagcaaa taatttatca
     3301 aaccataaat gaaatttatt aataatgtta taccacttta atcggctctc atgttttgct
     3361 ctgtcttctt tgaaatttat atattttgct gaaagtacag gattgattat cattgctaca
     3421 aacaacgagg aaaacaaaca tactatcaat gtaatgggaa gatatttcat aaattcacca
     3481 acaatacccg ggaaaaataa cagtggagca aatgaagaaa taatagtgaa tgttgcaatt
     3541 gtaacaggta cctgaacttc cctgggcccc tctattgcag catcatgcgg attatatcct
     3601 tcggtttcct gtagcctgta tatattttct gtaactacaa tagcatcatc tacaataatt
     3661 ccaagcacaa gtataagtcc aaacaaaaca acaatattca tggtaattcc taatcctgct
     3721 agtacaataa atgatattaa aaatgaaaat ggaattgaag atgcaaccag taatgaattc
     3781 ttaaaaccca ttgcagctag taagatcaaa catactaaca ggaatccggt aattattccg
     3841 ttctctaatt catgaactgt atttttaata cttatagatt catcacctgt aaaacttact
     3901 ttaagtcctt ctgggaaagt agattctttt tcttttacaa gtgatttaat ttcatcggcg
     3961 atacttataa tattttcacc acttcttttt ttaataacta ttgtaactgc ttcattaccg
     4021 ttttctcttg caagagttgt tctctcttta taatcatata ttacattcgc aacatctctc
     4081 agatatatag gctttccatt atcagacttt atgatcagat ctctgatcaa ttcaggatcc
     4141 tcatattctc ctggaactct tacaagataa ttttgcgatc ctatatcaac acttccaccg
     4201 ggaatactta aattctcatt tcctatcttt gttgtaatat catttaaagt aaggttgtaa
     4261 tatttaagac ggtccgcatc tacatttact ttaacttcac gttcaagtcc gccttttaca
     4321 tcagcactca atactccggg aattccttct atatcatcac tgatattatc tgcaatatcc
     4381 tttaacctgg ctaaaccaag attacccgaa agattaatat acagcatcgg ctgttcggaa
     4441 agatttattt cattgattga aggctcttca atatcctgcg gcatttttgt ctttgctatt
     4501 gcaactttat ctcttacctt ctgcaaagca tcatcaataa ttacatcagg attaaattca
     4561 atatttacca ttgagaaact ttcctgtgat attgatgtga tcttttttac atcacttatt
     4621 cctttgacct ctttttcgat ctcctgtgtt acaagacttt ccatgtcctg aggcgaaaca
     4681 ccaaagtata atgttgatac aaatatattt ggaataatta tcgaaggagc tgactctttg
     4741 ggaatagaat tataagagga taaccccact atgattataa ttaccattaa aatgtaaaca
     4801 gtaactttat tgtctacagc taattttgaa acactcatat taacaatttt tttaaaataa
     4861 ttaatttata atttcgactt tgtctccgtc tgaaacggac tgatatcctt ctgaaattaa
     4921 tacatcacct gaatttaacc ctgaaattat atgtacttta ttattgcttc tgccgcctaa
     4981 cgtaatgatc ttctttcttg cagttccatt ttccagaaca taaacaaatt tatcatttcc
     5041 aaaatctatg atcaaatcct gaggtaagac aattgcttca gttatcattg atttttcaat
     5101 taaaatattt gcactcattt caggttttag tttaccgtct ttatttggaa ttaccatctc
     5161 tatctcaaat gttctgttct tttcacttaa agttggtgat acataattca ctgatcctgt
     5221 aaactcttct cctggatata tatcaaaagt tatttttacc aatgagcctt tttttacttc
     5281 ttctacaaat ctttcaggaa tacctgcaga aatttttact tttgaaatgt ttacaatgtt
     5341 taaaatcgga gcacctgtag cgcaaacttc tccccggctc ataaatttct gatcgacaat
     5401 tccatatatc ggtgaagtaa tatatgattt tgaaagtctt gtctctatca ggttaagact
     5461 tttctctgca agctctaact gaaattttgc attcgtaaaa tcctgttcag ttgagacact
     5521 ttcataatat aatctctcaa ttctttcgta attacttttt gcgagctcat attgagtttc
     5581 tgcctgttca tatgaagcaa aatcaatatc ttttttaagc ttgacaagtg tctgccctct
     5641 gcttacacgg ctgcctttgt caaacggcat atatgtgatc agtccgcctt cctcagcaga
     5701 gatctttgca gtttcaaatg gttttacagt tccgaccact ttgtaagttt ccaaaaattc
     5761 acttgtaact atttctgaaa ctttcactgc ctgtaaagtt ttttcgtttt cagttttagt
     5821 ttctccgtca tctccgcatg atgaaatgat cattccaaag gacaaagtaa aaaagagtaa
     5881 cattatataa atgatatttt tcatgttaat tatattagat ttaatatttt atttttcaag
     5941 taatttttct aattctgctt ttgcaatatg ataatcatat actgcctggt aatatccgag
     6001 tctggaatta tataatgtca gttcagcatc ctgaacgtca atctgattta atacaccttc
     6061 tctgtaactg ttattggcta agtctaaacc tctttcagcc agtgttacag tttctcgggt
     6121 tgataaaatt ctttgcttag catcttcaag tgagattaat gctgcttccg atcttagctt
     6181 taatttctgc tgtacatcaa ttaaagattc ctgagatatc tttacatcaa gttttgcctg
     6241 atctacttta taggaattcc tgaataagtt cagatcccat gtcaatccaa taccggcatt
     6301 aaaggtatta taaaatctgt attttgatat tggcacatta tcattttcct gagaaattaa
     6361 ttcatatcga ccgtataaaa ataatttggg taagtaataa gcttgttgga catttactaa
     6421 ttcctcattc acttttttat ttagtcttaa ctgtcttact gtaacataat tttcggagat
     6481 cctcttaatc aactgatcag tactttccca gatttcagta ctgtcatatg taagagaatc
     6541 aattatttca atattatcat taacctttat cccgatggca ttggaaaata atttcttact
     6601 gatatcaaaa tctctttcag attttaataa aattggtctt acattttcga cttttactct
     6661 tgcccttaga aaatcaaatt ctgttgctac accgtttctg tatcttgatt ccacaactct
     6721 gaaatttgcc tcagcattat cataagtgat tttatttaca tcgacaattg cttttgacaa
     6781 taaaacgctg aagtaattat tctttacagt atttgcaacc tcattctcct tcagctcgac
     6841 attctcactt tgtatttttg aataatattc cgcaattcgg atacctgaaa ataccggcgt
     6901 tcctaatatc ggtattggtt ccgaaatatc aataccggta ttaaaattat tatctgaacc
     6961 tacttcaaaa gtctcaccaa atagatttaa tacctgcttt ttaaatgacc tgcttaacct
     7021 ggtgtccata gtcaggctgg gtagcaaatt ttcactgtaa acttcagata ctattttttc
     7081 agctttaagt ctttcaaatc tggcatttat cacatcagag ttgtctctta atgctaagtt
     7141 aactgcctgt tcaatagatg ttatgattag tttctgttgg gaaaaaactg gttcaacccc
     7201 aactaccagc acacagaata acagtattaa taatttttta aaatgtgttt tagtcatatt
     7261 aaattaaagt tttatttctt ttgcaaataa gtttttttcc ttgttgaaaa tatttttccc
     7321 taattgggtt aaaattccat ttaaaagcat ttcgtatgtg atcctaaaag cattttgtat
     7381 tgtaaattta ttattgatta aaaactcagg ctgcattact cctgatatag aatgtataaa
     7441 tgtacttata attattgttg ctggataatt ctcgataagc ttttctttct tgccctgttt
     7501 aataagtttt gacaatattc cgttaatttt atctgtacgt ctttcattga tatttttaag
     7561 gatatgtgga tgatgtactt tcagatcatt cagccattta tcactaatgt taattgtaaa
     7621 attactgtac atattaagaa gcatgataaa ctttaaaact acattacctt ctccttctac
     7681 tatatcatca acattattat atatcacttt ggttgtatgg tcacagatct cttcaagcaa
     7741 attttccttg gatgggaaat gtttgtaaat tgttttcttg cttatacaca tttcttttgc
     7801 aacttcatcc atagatgttt tgtaaaatcc ttctttgtga aatttatgtg tgcaaaagat
     7861 aagtatcttt tcccgctctt tgatttgttt ggatatttta agcaatagaa actatattcg
     7921 ttttatgagt ttccaaaata gtaaaaatta tttttacctg caaggcattg tttgtaatct
     7981 tttatggata cattataaag tttcctgtca ggattaaatt ttcacagaac ttactacgga
     8041 ggataaattg gatttgttat tttcggacat tttgagaatt ttgtataata aaacagcaaa
     8101 aaaaatgcgg aatcttattc cgcattaatt tttcgaatgt gagaatctat tattctattt
     8161 acctacttca ccttcaaatt gaaattcatc ctctgtttca gttccatcat tcttcttgct
     8221 aattttgatc attatttttc cgtcttttct tatcacattt atatcttcag gattaatatt
     8281 tttgtctttc aaatcttcaa caaccatctt cttgatctct tcgtcagatt tcccttcaaa
     8341 tctgtcgact tctccatcag ctttgtttct gaactttctg acttccattg ttttgttatt
     8401 ttcatctcca tcctgaattg taagatcaaa acctttttta tttttaattt cttcctcagg
     8461 aattatgacc tttgaaattc ttcttccgtc attatttctt tcaaaactga tctgaacacc
     8521 ggaaacacca ttttgctcaa gctgtgatga gatctgattc ctgagttctt catctgacat
     8581 atttgtagca tttatatcta ttttgaaaat ttgaatcagt gccagtgagt atacaggtcg
     8641 cgttatttgt tcattcagtg gtacaaggtt tacagtcaga attccttcca ttgtatttaa
     8701 ctgctcaaga tacttttttg aatttgcatg atcttccttt gaaacaatca gagtatattc
     8761 agcaattgaa ttcccattat ctgctgtact aatttttatt gctgaattct ttgtccattc
     8821 gagactgctt aatctgctga ttgtttttgt atcattttga tctactgtcc atttaattac
     8881 atcagcgaca ggttcctgtt gagtcacagg atagttacag gctgtcacta caaatgctaa
     8941 tataagaaaa gcatatgcta atttgatctt aggtgatttc atattaaaaa atttcctttc
     9001 gtgattagat gagattttaa aagttttttg tgaatcaact tttcggagcc atttctcaac
     9061 ttccggaaat gaattttcag tattttcatt tcttagattt tcgaacatgt catctgattt
     9121 attgaaatta tcagaactat ttattttcct gtcccggtat gagtttgatt gtttcattgt
     9181 ttaaatctcc tgaataaatt gatttttgat tttctgaatt tttgttttgt gttatattat
     9241 tttcttcatt ctcaataaat tctctgagct tttttcttgc ccggcttagt ct
//
LOCUS       DAORVQ010000129         9240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4064_length_9240_cov_5.719543, whole genome shotgun sequence.
ACCESSION   DAORVQ010000129 DAORVQ010000000
VERSION     DAORVQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_9240_cov_5.719543"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="PLX80_10260"
     CDS             complement(<1..1579)
                     /locus_tag="PLX80_10260"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21213.1"
                     /translation="MHPEIFLSYSHKNFEIADSIDKDFEKIGIRLIRDIRDAEYMQSL
                     KEFMLKIRETGYALLIISDEYLKSENCMYEVTELLKESNFQDRILLIILENAKVFDTS
                     GKLSYAKYWNQQFDDLNNEIKELPRDKSIKLSEELKKIDNINSCIDDFLSLISDRKSV
                     KLTNLKNNNYKEILDIIKYKKIDLAEELFRIIQMIDTKKYEEIDLELETFTNNNPNYP
                     DGYFVRANIAIDNKQNVKAKYLLEKAIEINPEYFQAYNNLALLLEINFFDIEKAKVYF
                     EKAIEINPKDTISLNNFALLLINFFNDKVSAKNYLEKAININPNYIEAISNLALLLID
                     SFNDYQGAKSLYEKAIEINPYFSDSYYSLAQLFDFKLENIEEAKKQYEKAIEINPNIV
                     DAYLNLAHLLAYKLNDKESSLELYKKAIELFPKNEIAHFNLASLLDKKFEDKIGAKEH
                     YEKALELNPNFYQGYNDLALLLENKFGEFEKATEYYKKAILVNPKDDKAHYNLGNLLV
                     NKFDNIEDANYHYLVSLS"
     gene            complement(1781..2851)
                     /gene="prfA"
                     /locus_tag="PLX80_10265"
     CDS             complement(1781..2851)
                     /gene="prfA"
                     /locus_tag="PLX80_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQY21214.1"
                     /translation="MFEKLEKVKSRYKEISDLLSDPATSGDQTEIIKLSKEYSGLEDI
                     VNAYNNYLGIKKELEDNKQLLYETSDQEMKELARQEQEILEIKLSDVETEIKELLIPE
                     DPADNKNAILEIRSGTGGEEASLFAADLYRMYSRYFEKKGWKMETIDFNDSSNPGGIK
                     EVVLNVSGKNIYGDLKYESGVHRVQRIPDTESKGRVHTSAATVAVFPEAEVFDVVIND
                     NDLKIDVFRSGGAGGQNVNKVETAIRITHLPTNIVVQCQDERSQLKNKMSAMKVMRSR
                     LYELELAKRDNELSSQRKNMIKSGDRSDKIRTYNFPQNRLTDHRIGLTLYNLSEIMEG
                     DLDEIIDKLKTADRAERLASAE"
     gene            complement(2905..3270)
                     /locus_tag="PLX80_10270"
     CDS             complement(2905..3270)
                     /locus_tag="PLX80_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21215.1"
                     /translation="MTPDLQSAKTFFLVSAIINILGFLGWGTSTVIGGVFTCGLGCLM
                     GFLPIINIVSSIMDFIAYNKLSNLNQKGTFGTVQTAAIFQIVTILTGNVVSFIFGIII
                     LNNLSNEENKNFLKERDIF"
     gene            complement(3439..3777)
                     /locus_tag="PLX80_10275"
     CDS             complement(3439..3777)
                     /locus_tag="PLX80_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21216.1"
                     /translation="MSESVRNLDITPEEIESGKTMAIIAYLIFFIPLLMDDMKKNKFV
                     MFHTEQSIILLLLNIAAGIIGTITCGIGLVLYIPWVIFLIMGIMNAAGGKVVPLPLIG
                     QYGEKFNLVK"
     gene            complement(3839..4255)
                     /locus_tag="PLX80_10280"
     CDS             complement(3839..4255)
                     /locus_tag="PLX80_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21217.1"
                     /translation="MKKLLQIKILLILTFTAGMLYAAVTLQYFTARDNAGGVMVEWKT
                     GDEGGTAKFEIERSADSPDNFIYMNFKEATGNNSYYSYQDNSVDIGSGRNSELYYYRL
                     KCIEANGNYTYTNHITVTHTVSGVRSTWGSIKAIFR"
     gene            4539..4781
                     /locus_tag="PLX80_10285"
     CDS             4539..4781
                     /locus_tag="PLX80_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21218.1"
                     /translation="MIKYDFAKEGFVKIKIYDLTGREIRSLVNELKQAGSYSTVFNGA
                     NLSSGIYFYKLETENFRQIKRMVLVKKIQTFKNIYL"
     gene            5127..6146
                     /locus_tag="PLX80_10290"
     CDS             5127..6146
                     /locus_tag="PLX80_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21219.1"
                     /translation="MKKIGILFGQENTFPQAFVDKVNEKNEKDIIAEFVDVDKVVQGE
                     SCGYDVIIDRISQDVPFYRGFLKNEAITGTAVLNNPFWWAADDKFFNNALATKVGVPV
                     PKTVLLPSNQNPPDTTGESFRNLSFPLDWEGIFKYTEFPAYFKPFSGGGWKSVYKVED
                     PSDFFEKYNETEQLVMMLQEEIQFDLYFRCYCLDRKDVLIMPYEPRNPHHLRYSSNAE
                     PASKEMLALIKDYVLRLNNALGYDFNTVEFAVRDGVPYAIDFCNPAPDADINSVGQEN
                     FDWVVEAAANMAIRRAKNHKEGMNNLTWGNFVKQFSKDLSLSNGSAKKPEKAKPASKS
                     KKAKS"
     gene            6168..7274
                     /locus_tag="PLX80_10295"
                     /pseudo
     CDS             6168..7274
                     /locus_tag="PLX80_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560151.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
     gene            7436..8458
                     /locus_tag="PLX80_10300"
     CDS             7436..8458
                     /locus_tag="PLX80_10300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQY21220.1"
                     /translation="MKNSFIADIEFDKELSEGANNAINVCLRLKPEERITIITDNDTL
                     EVACALVHEIKKVGSEYSLFVIEDYTSRPSPNMPQPILDDLAKSQVSIFCAQAQTGEL
                     SMRIQMTSVVNSHKIRHGHMVNINKQIMLEAMRADYLAIDDLSQKLITRARKARYIKA
                     KSDAGTDIVAEFSHSLNWLKTSGIISVDKWGNLPGGEIFTSPKSVDGLFVVDGVVGDY
                     LCQKYGDLKNNPLYIEIEDSRIMNMTCKNTALLDDMTAYTMTDENSNRVGEFAIGTNI
                     GINKVIGHILQDEKLPSIHMAFGHPYSEHTGADWVSTTHIDCVGIDINIWLEDDQVME
                     KGKFLI"
     gene            8570..>9240
                     /locus_tag="PLX80_10305"
     CDS             8570..>9240
                     /locus_tag="PLX80_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21221.1"
                     /translation="MIKSYREKYNSEFSNENYKSMVEELDSAFGTKIDFKIAETPIFL
                     PDEFRDELIKASDEIVRMLQSDEFKEYSKKALPDNVAVPNEDEHPSLLAIDFAVCKDE
                     NGKLIPQLIELQGFASLYCYQELLNSEMRKHFDIPEGLTSHFSGLDHDSYIQLLKETI
                     IGDTDPENVILLEIEPEKQKTYIDFLCTEKFLGIKVVCLSKVMKEGKQLFYDNNGKKT
                     LIKKI"
BASE COUNT         3127 a         1533 c         1537 g         3043 t
ORIGIN      
        1 tacttaatga aactaaataa tggtaatttg catcttctat attatcaaat ttatttacta
       61 acaaatttcc taaattataa tgtgctttat catctttagg atttactagt atagcttttt
      121 tataatattc tgttgccttt tcaaactcac caaatttatt ttctaataac agtgctaaat
      181 cattgtatcc ttgataaaag ttaggattta attctaaagc tttttcataa tgttcttttg
      241 cgccaatctt atcttcaaac tttttatcta ataagcttgc taaattaaaa tgagcaattt
      301 catttttagg aaataattca atagcttttt tataaagttc taatgaactt tctttatcat
      361 ttaatttata agctaacaga tgtgctaagt ttaaataggc atcaactata ttaggattga
      421 tttcaattgc tttttcatat tgtttttttg cttcttcaat attttccaac ttaaaatcaa
      481 ataattgagc taaactataa tatgaatctg aaaaatatgg gtttatttca attgcttttt
      541 catatagact tttcgctcct tgataatcgt tgaaactatc aattaataaa agagccaaat
      601 tggatattgc ttctatataa tttggattga tattaatggc tttttctaaa taattctttg
      661 ctgaaacttt gtcattaaaa aaatttatta ataataaagc aaaattattt aatgaaatag
      721 tatcttttgg attaatttca attgcttttt caaagtaaac ttttgctttt tcaatatcaa
      781 aaaaattaat ttccaataac aaagccaaat tattataagc ttgaaaatat tctgggttta
      841 tttcaattgc tttctctaaa agatattttg ctttgacatt ttgcttgtta tctattgcaa
      901 tatttgctct aacaaaatac ccatcaggat aattagggtt attatttgta aaagtttcta
      961 attctaagtc aatttcttca tatttttttg tgtctatcat ttgaataatt ctaaataact
     1021 cttctgctaa atcaatcttt ttatacttaa ttatatctaa tatctcttta taattattat
     1081 tttttaaatt tgttaattta acagattttc tatcacttat taaacttaga aaatcatcaa
     1141 tacaagaatt aatattatct atttttttaa gctcttcact taattttatg cttttatctc
     1201 gaggtaattc tttaatttca ttatttagat catcaaattg ctgattccag tacttagcat
     1261 aacttaattt accagaagta tcaaatactt ttgcattttc taaaatgata agtaaaattc
     1321 tatcttgaaa atttgattct ttcaataatt ccgtaacttc atacatacaa ttttcagatt
     1381 tcagatattc atcactaata attaaaagag cataaccggt ttctctaatt tttagcatga
     1441 actctttcaa actttgcatg tattcagcat ctcttatgtc tctaataagt ctaattccaa
     1501 ttttttcaaa atctttatct attgaatcag ctatttcaaa atttttatga ctatatgata
     1561 aaaatatttc aggatgcatt gttaatattg gatttttaat gtaaataaaa taacatttga
     1621 tataagaact aaaaattcaa attcattctt tataatataa ttccgcaata atatttgtaa
     1681 ttttataaaa aaattaattt ttaaccaata ttaaagtcgt ttactttctt atattattca
     1741 aaaaagattt tcctttgagt cttagagtct cgaggcaaca tcactccgcc gaagcaagtc
     1801 tctcggctct atccgccgtc ttaagcttgt ctatgatctc gtccagatca ccttccatta
     1861 tttcagaaag attatataag gtaagtccga tcctgtgatc ggtcagacgg ttttgcggga
     1921 aattgtatgt tctgatctta tcacttctgt ctccggactt gatcatgttt tttctttgtg
     1981 aggaaagttc attgtctctt ttggcaagct cgagttcata gagtctcgat ctcattactt
     2041 tcatcgcgct cattttattt ttaagctgcg atctttcatc ctgacactgc actacaatat
     2101 ttgtcggaag gtgagtgatg cgtatcgccg tttcgacctt atttacattc tgtccgcctg
     2161 cgccgccgga tctgaatacg tctatcttca gatcattatc atttattaca acatcaaaaa
     2221 cttcagcttc gggaaaaaca gcaactgtag ctgcggaagt atgaactcta cctttgcttt
     2281 ctgtatcagg tattctctga actctgtgaa cgccgctttc atatttcaga tcgccgtaaa
     2341 tattttttcc gctgacattt aaaacgactt cctttatccc gcccggattg gatgaatcat
     2401 tgaaatcgat cgtctccatt ttccagcctt tcttttcaaa atatctcgaa tacattctgt
     2461 aaagatcagc agcaaagagc gatgcttcct cgccgcctgt acctgagcgt atctcaagaa
     2521 ttgcattttt attgtctgcg ggatcttcgg ggataagtaa ttcttttatc tcagtttcta
     2581 catcagataa tttaatttca agaatctcct gctcctgacg tgcaagctct ttcatttcct
     2641 gatcggaagt ttcatagagt aattgtttat tatcttcaag ttccttctta ataccgagat
     2701 aattgttata ggcatttaca atatcttcaa gccctgaata ttcttttgac aatttaataa
     2761 tttcagtctg gtcacctgat gtagcagggt cagaaagcag atctgaaatt tctttgtaac
     2821 gggattttac tttttcgagt ttttcaaaca taaattaaaa cggctttctg ttgatttgta
     2881 ttaaattgaa aatataaaat tgatttaaaa tatatctctt tctttcagga aatttttatt
     2941 ctcttcatta ctcagattat ttaagatgat aattccgaat ataaagctaa caacattacc
     3001 ggtaagaatg gtaacgatct gaaaaatggc tgcagtctga actgtaccga aagtaccttt
     3061 ctgattgaga ttgctgagtt tattataagc gataaaatcc attatactgc tgactatatt
     3121 tataattggc agaaatccca tcagacatcc aagtccgcaa gtaaacactc cgccgatgac
     3181 agtggaagtg ccccatccga gaaatccgag aatgttgatt attgctgata caagaaaaaa
     3241 agttttagca gattgtagat caggtgtcat aatttttgat tttatttatt tacttatcaa
     3301 aagttgttaa gcaggttatc cttacgtttt catttaatca attgttagaa aataaaattc
     3361 agatattaat tcacataaaa caaaaaagca atctgctttt aataagaaga ttgctttaag
     3421 aaaaactttt tgaaaaagct attttacgag attgaacttt tctccgtatt gtccgataag
     3481 aggaagagga accactttcc cgccggctgc attcataatt cccataataa gaaagatcac
     3541 ccatggaata taaaggacta atcctatgcc gcaagtaata gtaccaatga tacccgcagc
     3601 aatatttaaa agtaaaagaa taatggattg ctcagtatga aacatcacaa acttgttttt
     3661 tttcatgtcg tccatgagca ggggaataaa aaaaatcagg taagcaatta tagccattgt
     3721 ttttcctgat tcgatctctt cgggtgtgat atctaaattt cgtacagatt cagacatatg
     3781 tatctccttt caaaggttaa gtaataatta attaattaaa tgaaaaatgg agccggaatt
     3841 atctgaaaat agctttaata cttccccagg tacttcttac accggatact gtatgcgtaa
     3901 ccgtaatatg atttgtataa gtataatttc cattagcttc tatacacttg agtctgtaat
     3961 aatagagttc ggaatttcta ccgcttccaa tatctactga gttatcctga taagaatagt
     4021 atgagttatt acctgtggct tctttaaagt tcatgtaaat aaaattgtcc ggtgagtctg
     4081 cgcttctttc gatctcaaac ttagcagttc cgccttcatc tcctgtcttc cattccacca
     4141 taacgccgcc tgcattgtct ctggctgtga aatactgcag tgtcacggct gcataaagca
     4201 ttcccgctgt aaatgttaga atcagtaaaa tttttatttg taacagtttt ttcatgattt
     4261 tgataaatat aaacccaata atcgggaatt tcaagtttta attattagga tattgaaaaa
     4321 aaatttaacc cgaattttca ttataaatcc cgtttttacc acgaaggcac aaagacacaa
     4381 agtttttaat gatagactta tacagcagtt gtttatgttt tcaacaggag aaataataat
     4441 tccagccctt attgtcatac cctcgaaagc gggaatctcc aaatgaaata ctcagcaaac
     4501 tcttttagca attagtgaca tgaattttca catccacagt gataaaatat gattttgcaa
     4561 aggaaggatt tgtgaaaatt aaaatttatg atctgacagg cagagagatc cggtcacttg
     4621 taaatgaact caagcaggcc ggttcgtaca gtacagtctt taatggagca aatctttcaa
     4681 gcggaattta tttttataaa ttagagaccg aaaacttcag acagataaag cgtatggtat
     4741 tggtcaagaa aattcagact tttaaaaata tttatcttta gccggattga tttgtttgat
     4801 ccggcttttg ttatatcgat aatatttaag aatacaaaaa taatatttaa attattttat
     4861 gccattatag gtgtctttta aaaagtaggc aattcgtaac cggacatcag gaatcgattt
     4921 tactgctgat taaaatagat ttacaaatgt ggaaaaattc agcgtttaaa ttatcactta
     4981 attttactaa attatctact tatcaattta atttattttc actgcgaaac tattttacat
     5041 ttcagatatt cattcgaaaa atgaagaaat tgatttattt tttacaaatc aaataattta
     5101 aaaacttaag gagttaatta attctaatga aaaaaatcgg aatattattc ggacaggaaa
     5161 acacctttcc gcaggcattt gttgacaaag taaacgaaaa aaatgagaaa gatattattg
     5221 cagagtttgt agatgtggac aaagtagtac agggtgaatc atgcggatat gacgtgatca
     5281 tcgacagaat ttcacaggac gttccattct acaggggatt cctgaagaat gaagctatca
     5341 caggaacagc agtattaaac aacccctttt ggtgggcagc cgatgacaag ttcttcaaca
     5401 atgcacttgc aacaaaagta ggcgtacctg ttccaaagac agttttactt ccgtcaaatc
     5461 aaaacccgcc tgacaccaca ggtgaatcat tcagaaattt atcattccct ctcgactggg
     5521 aaggaatttt caaatacaca gaatttcctg catacttcaa gccattttct ggaggcggct
     5581 ggaaaagcgt ttataaagtg gaagacccgt ccgatttttt tgaaaaatat aatgagaccg
     5641 aacagctggt aatgatgctg caggaagaaa ttcagtttga tctgtatttc agatgctatt
     5701 gcttagacag gaaagacgtt ctgataatgc cttatgagcc gagaaatcct catcatctca
     5761 gatattcttc aaatgccgag cctgcttcaa aagagatgct ggctttgata aaagattatg
     5821 tattaagatt aaataatgcg cttggttatg atttcaatac agtagaattt gcagtaagag
     5881 acggcgttcc atatgcgatc gatttctgta atccggcacc tgacgctgat ataaattccg
     5941 tcggtcagga aaattttgac tgggtagtcg aggcagcagc taacatggct atcagaagag
     6001 caaagaatca taaagaaggt atgaacaatt taacatgggg aaattttgta aagcagtttt
     6061 caaaagatct atcattgtca aacggttctg ctaaaaaacc tgaaaaagct aaaccggctt
     6121 caaaatctaa aaaagctaaa tcctaatatt tttataaaaa aggaaaaatt atcacagacg
     6181 aaaaatatac attagggata gaagaggaat ttcagatcat tgatccaatt tccagggagc
     6241 ttcagtctca tgttcattct atttttgaag aaggtaaaca tcttttaaaa gagaatttca
     6301 aacctgagat gcatgcatct gtcatagaga caggtacaaa catctgcaat aacatttagg
     6361 aggcaagagc tgaagttacc aatctgagaa attctctggc aatgctttca gaccagtacg
     6421 gtcttagaat tgcagcagca ggaacgcatc cattctctca ctggagggat cagaaaatca
     6481 cagatcatcc gagatataat gagataataa atgaaatgca ggacgcagcg agagcaaatc
     6541 tgatctttgg aatgcatgtg catgtaggca ttgcaaacag ggagatcgga attcagataa
     6601 tgaatgctgc gagatatttt ctaccgcata tatttgcatt atcgacgaac tcacctttct
     6661 ggctcggtag agatacagga tttaaatctt acagagtaaa agtctttgat aaatttccga
     6721 ggacaggaat tcctgattca ttcagaagtc tttcagagta tgacagttat atcaatctgc
     6781 ttattaagac aaactgtatc gacaatgcaa aaaaggtttg gtgggatatc aggacacatc
     6841 cttttttcaa tacacttgaa ataagaattt gcgatgtaca gcttagagtc gatgagacta
     6901 ttgcacttgc agcgcttatt caggcagtgg tagtaaagct tcacaaactc attcagcaga
     6961 atctcggatt cagactttac acacgtgctc tcatgatgga aaataaatgg agagcagcga
     7021 gatacggcat cagcggaaag cttattgatt ttggcaagca ggaagaagtg ccatttatag
     7081 aacttgcaaa agagcttctg gaatttatag atgatgtagt ggatgaactt ggcagcaggg
     7141 aagagataaa ttacattttc agaatgatgg aaggcggcac aggtgctgac aggcagctta
     7201 aagtctttga agaaagcggt ggtgatttta ataaacttgt cgattacata gtcgatgaaa
     7261 ccaacgcagg tctggattac aaaccaacta acattgtaag taaagaagac aaaccaacta
     7321 atattgaaat aaaaaaagaa aaacaaacaa aaccaactaa cattaccaac actactaaca
     7381 aaaaaggacc aaaagtaaaa aacaaaactt cagaaactaa aaaatcaaaa tagaaatgaa
     7441 aaattctttt atagctgata ttgaatttga caaagaactg tcagaaggag caaataatgc
     7501 tatcaacgta tgtctgaggc tgaagcctga agaaaggata accattatca cagataatga
     7561 tacacttgaa gttgcatgcg cacttgttca cgaaataaaa aaagttggtt ccgaatacag
     7621 tctttttgta atagaagatt atacatcaag accttcacca aatatgcctc agccaattct
     7681 cgatgacctt gcaaagtcgc aggtcagcat tttttgcgca caggcacaga caggtgaact
     7741 cagcatgaga attcaaatga catctgtcgt aaattctcac aagatcagac atggtcatat
     7801 ggttaatata aataagcaaa taatgcttga agcaatgcgt gcagattatc ttgctattga
     7861 tgaccttagt cagaaactga tcacgagggc aagaaaggct agatatataa aagctaagtc
     7921 tgacgcaggt acagatatag ttgctgaatt ttcacacagt ctgaactggc tgaaaacaag
     7981 cggtattatc tctgttgata aatggggaaa tcttccgggc ggtgaaattt ttacttcacc
     8041 aaaaagcgta gatggacttt ttgtagtgga cggtgtggta ggagattatc tctgccagaa
     8101 atacggcgat ctgaaaaata atcctttgta tatcgagatc gaagattcaa ggatcatgaa
     8161 tatgacatgt aaaaatacag ctcttcttga tgatatgaca gcttatacaa tgacagatga
     8221 aaacagcaac agagtaggtg aatttgcgat cggcacaaac atcggcataa ataaagtcat
     8281 aggtcacatt cttcaggatg aaaaacttcc gagcattcat atggcattcg gacaccctta
     8341 ttcagagcat acaggtgctg actgggtctc gactacgcat attgattgtg taggaattga
     8401 cataaatatc tggctcgaag atgatcaggt aatggagaaa gggaaatttc tgatttaatt
     8461 tttactatta ataatattta ctgatatttt tcatattgtt aattatacaa ataagattta
     8521 ttgccatcat aaattttttc atatttcaaa catttcattt ctatattaat tgataaaatc
     8581 ttacagagaa aaatataatt ctgaattcag taatgagaat tataaatcaa tggtcgaaga
     8641 acttgattct gcttttggta caaaaataga tttcaagatc gcagaaactc ctatcttcct
     8701 tcctgatgaa ttcagagatg agttaattaa agcttcagat gaaattgtaa ggatgcttca
     8761 gagtgatgaa ttcaaagagt attccaagaa ggcacttccg gataatgtag cagttcctaa
     8821 tgaagatgaa catccttctt tactggctat tgattttgca gtttgtaaag atgaaaatgg
     8881 taaactgatc ccgcaattaa tagagcttca gggatttgca tcgctttact gttaccagga
     8941 acttctcaac agtgagatga gaaaacattt tgacattcca gaagggctga ccagtcactt
     9001 cagcggactt gatcatgaca gttatattca gttgttaaaa gaaactatca ttggagatac
     9061 tgaccctgaa aatgtgatac tgcttgagat cgaacctgaa aagcaaaaga cttatattga
     9121 ttttttatgt acggaaaaat ttctcggcat aaaagtagtt tgtctttcaa aagtaatgaa
     9181 ggaaggtaag cagctttttt atgataataa cggaaagaag acacttataa aaaagatcta
//
LOCUS       DAORVQ010000130         9222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4081_length_9222_cov_5.143995, whole genome shotgun sequence.
ACCESSION   DAORVQ010000130 DAORVQ010000000
VERSION     DAORVQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9222
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_9222_cov_5.143995"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1164
                     /locus_tag="PLX80_10310"
     CDS             190..1164
                     /locus_tag="PLX80_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21222.1"
                     /translation="MSNQKSILIFTESLIDYAGLFPPAKLDLNTSFNNFLNYQSGKNS
                     NMLSKFICPVKLLPDLYQLFNRKTEMIQPIEISLIITGGENLEAFKNSLKSDLELRSK
                     FIDETKSSFKVNNFEYKIPDEIIKNKDHKEISELLKFISDNTGSDHEIKLSFFAESTL
                     EGDWKSNIEFIADALEMHNENYDDTGFKLRTGGITKTAFPSSEQIAFAVGYCLKRNLE
                     MKFTAGLHHPIRHFDIGLDTKMHGFINVFTAGIIAKRHNISEHEMIKLLEDESAENFR
                     FTESGFSWNGYEIENEDIHFARSTFVRSYGSCSFDEPVDDLKKLNLLN"
     gene            1189..2445
                     /gene="fahA"
                     /locus_tag="PLX80_10315"
     CDS             1189..2445
                     /gene="fahA"
                     /locus_tag="PLX80_10315"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HQY21223.1"
                     /translation="MKSFIEIKSNSDFPIQNLPYGIFYTSENHSKRAGTAIGEYILDL
                     DAAEKNGLFEKKTFTGKDIFNTGSLNKFMSLGKNVWSEVRKAITELLDENNPLLRNNE
                     NLRSECLILQKDATLCMPSEIGDYTDFYSSKDHATNVGIMFRGKENALMPNWLHLPVA
                     YHGRASSVVLSGTDIIRPKGQTKADDAETPSFGPSRLLDFELEMGFFIGTGNELGNPI
                     RIENAEEHIFGMVLVNDWSARDIQKWEYVPLGPFLAKNFATSISPWIVTMEALEPFKT
                     KSEIQDPEPLPYLKRVKENSYDIKLDVILKTPGSEKEEILSESNFKYMYWDMCQQLAH
                     HTVTGCNMRPGDLLASGTISGPEKGTYGSLLEITWRGSEPVKLSNGDELKFLRDGDEL
                     IMRGYCEKDGLRIGFGEVTGKILPSN"
     gene            2448..3293
                     /locus_tag="PLX80_10320"
     CDS             2448..3293
                     /locus_tag="PLX80_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21224.1"
                     /translation="MNIMLKCVSVLLIVISVSSCSDNSQNGNNIVYDENFQYPYSLNS
                     FWYYSTKNAVVNIRPDSLKRLYKGDTASGYGFSSFVNDTVIENKNVRILKNEYSLDGH
                     SSVVREYFEQTDSGLIRLASLSNGSNLSPFRGNIKYYINGNYFNSLNEISFRYNNDLI
                     SNDSIVLIDNPPLKTIKYPLELNSEWELINNGFVRIQKKYTGFENIDISGKNFHCMKI
                     SRSYYFSGSLPDENFIVTDYFSAEGIVRRDLLIKNINVVNELGNLLGYVDLSEKTTVN
                     LFSIP"
     gene            3329..4390
                     /gene="selD"
                     /locus_tag="PLX80_10325"
     CDS             3329..4390
                     /gene="selD"
                     /locus_tag="PLX80_10325"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQY21225.1"
                     /translation="MDNEKVRLTQMVTSAGCAAKIFPDILSKTLKEITWDTNENVLVG
                     FEGKDDAGVYKISDNTALIQTTDFFTPVVDDPYTFGQIAAANALSDVYAMGGEPICAL
                     NIVAFPQKEDLNILKEILRGGADKMKESGAVIIGGHSIDITNIVYGLAVTGKINPNEI
                     KGNDSAKPGDILILTKALGTGILNNVIKFAQLEDKIYQQLISSMTRLNKDAAECMKLC
                     HANGCTDITGFGLAGHSMQMAVASKAVLKFFINQLPVLPGVEHAIGEKLLTRGDKSNR
                     NYTKDNTISEGEINIIKEHLLYDPQTSGGLLITVPKEHSNILLLELHKRGDIYAAIVG
                     EVLESTDQYKEGSVIFNYE"
     gene            4418..5626
                     /gene="mnmH"
                     /locus_tag="PLX80_10330"
     CDS             4418..5626
                     /gene="mnmH"
                     /locus_tag="PLX80_10330"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HQY21226.1"
                     /translation="MKDKELSDKEFIESVISDFSKDKGDDLSVKDLLKNYEIKYIDIN
                     ELHSDLKNIPNKILLIDARSEKEFSNASIPFAKNFPVLTNEERHYVGIIYKKYSQSAA
                     IKLALKYAVPKTEDLKKFIETNGTQNKKIIVHCWRGGGRSAYLSKMIYDLGFEVEVLS
                     GGIKSFRKEVNKFFELDKFPFELLEISGLTGCGKTELLNSVSQRLPVIDLEFAAKHFS
                     SLLGHIPFEIKGVKKVTGQSEFENNIYNQIRLNNSEHCAYDNLNYPFLIESESRRVGK
                     FTVPEVLYKSMESASSIKLTGSMSSRVNRIVGDYFSDKEKGIELMTDLFKTKGNFFKQ
                     QLSNKIFDELLILLEKGDVYDFTEIMIKDYYDKRYREKKKIPISEINSDNMESASDVL
                     IELYKKKYIF"
     gene            complement(6080..>9222)
                     /locus_tag="PLX80_10335"
     CDS             complement(6080..>9222)
                     /locus_tag="PLX80_10335"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HQY21227.1"
                     /translation="GDPNLVKKTDINLGLAVDLEKKDGSRSLIVPNIKKANQMDFCQF
                     FKAYNDIIDRSRKNKIEISDFQGTTITLTNPGTIGTVASTPRLMIGQGAIIATGAIDY
                     PAEYQAVTSEIITTIGISKIMNMTSTYDHRIIQGAESGLFLKELSGLLRGENNFFEEI
                     FEDLGIPMTPVKWFSDKTAEDLGRIDNLEEIERQAKAIQLINMFRVRGHLLANLDPLY
                     LKVQYHPELDPSNYGFTVWDYDREFITDGLGGLRTATLRTILDILQQTYCDKIGVEYR
                     HIQDPEQKKWLQDKMEPTKNLPSFSNEEKKHILFKLSEAENFEKFIDKKYIGHKRFSL
                     EGSETIIATLDHLLSSAADYSVEELVLGMAHRGRLNVLANIIGKSMSTIFSEFEGYID
                     PISSQGSGDVKYHLGASGEYETFHDKKIRVSVASNPSHLEFVNPVVEGIVRAKQMRMN
                     DLERNKIIPVLIHGDAAAAGEGIVAETLNLSQLHGYKTGGTIHIIINNQIGFTTSPID
                     ARSSVYATDVGKMIQAPIFHVNGDDPEATMLVTKLALEFRMKFNEDVFIDVFGYRRLG
                     HNEADEPAFTQPIMYKTIRSHPSVKEIYTKKLLSEKVITPEEIEISEKKIYDKMSESH
                     ESVQKFKADRPLAVTKEEILAVESNFDTFVPIDKLNEIVTAITTIPEDFHIHPKLKKF
                     IESRREFLDKDIEIDWAFAESLAYGSLLQEGIPVRLSGQDSSRGTFSQRHIILTDAEN
                     GDEIIPLNNVAPEKAKIEPLDSLLSEAAVLGFEYGYSTADPITLVLWEAQFGDFVNAA
                     QVIIDNFIASSYTKWGLPNNIVMLLPHAQEGQGPEHSSARLERFLTICADDNMIVCNL
                     TTSAQYFHLLRKQTKHRKRRPLVIMTPKSLLRLPEAKSIKEDFTSGKFIEIIDDVPEN
                     KSDIKSVIICSGKIYYDLRKYQKENEHFDTAIVRLEQYYPYKTDVMTEILASYKNADR
                     VIWVQEEPKNMGAWNFIAILLQNDLANWQKLYCVSRKESSSPAEGSFSKFTKNQEEIM
                     RKSFSNDLVQVFDFHN"
BASE COUNT         3121 a         1518 c         1559 g         3024 t
ORIGIN      
        1 aataaatcaa tcaaaaaatt gatatcaaga ttctgaaaaa ttttaaattt atgatctaaa
       61 actgctattt ttgatctgat tttatgtttt acaattaata attaattaat cagtcagata
      121 gatttaataa taaatttatt tagtttgaaa gaatgactat tttaaaaaaa tctaaaaatt
      181 aattatcttt tgtcaaatca aaaaagtatt ttaatattta ctgaatcatt gatcgattac
      241 gcaggtctat tcccgcctgc aaaacttgat cttaatactt cttttaataa ttttctcaat
      301 tatcagtctg gtaaaaattc taatatgctt tcaaagttta tttgcccggt aaaattgctt
      361 cctgatctat atcaactctt taataggaaa actgagatga tacaacctat agaaatatca
      421 cttataatta ccggtggtga gaatttagaa gcttttaaaa attcactaaa gtctgatctt
      481 gagttaagat caaaatttat tgatgaaacc aaatccagtt ttaaagtaaa taattttgaa
      541 tataaaatac cggatgaaat tataaagaat aaggaccata aagaaatttc agaactgtta
      601 aaatttattt cagataatac aggatctgat catgaaatta aattatcgtt ttttgctgaa
      661 tcgactttgg aaggtgactg gaaaagtaat atagaattta ttgctgatgc ccttgagatg
      721 cataatgaaa attatgatga cacaggtttt aaacttagaa ccggtggtat cacaaagaca
      781 gcatttcctt cttccgaaca aattgctttt gctgttggat actgcttaaa aagaaatctt
      841 gaaatgaaat tcaccgcagg tctccatcat ccaataagac attttgacat tggcttagat
      901 acaaagatgc atgggtttat aaatgttttt actgcaggaa ttattgcgaa gcggcataat
      961 atttcagagc atgaaatgat taagttactt gaagacgagt cagcagaaaa ttttagattc
     1021 accgagtctg gatttagctg gaatggatat gaaattgaaa atgaagatat acattttgcc
     1081 agatcaacat ttgtaaggtc ttatggaagc tgcagctttg atgagccggt agatgacctg
     1141 aaaaaactaa acttactaaa ttaaaatttt atgagagaaa ataatactat gaaatcattt
     1201 atagaaatta aaagcaattc ggatttccca atccaaaatt taccttacgg aatattttat
     1261 acttctgaga atcattccaa aagagcaggt actgcaatag gagaatatat acttgacctt
     1321 gatgctgctg aaaaaaatgg tttgtttgag aaaaagactt tcactggcaa agatattttt
     1381 aatacaggat cactgaataa atttatgtca cttgggaaaa atgtctggag tgaagtgaga
     1441 aaagcaatta ctgaacttct tgatgaaaat aatccacttc ttagaaacaa tgaaaatcta
     1501 agatcggaat gcctgatact tcagaaagat gcaactttgt gtatgccttc agagatcgga
     1561 gactatacag atttttattc ttccaaagat catgctacca atgtaggaat aatgtttcgc
     1621 ggaaaagaaa atgcattaat gccaaactgg cttcaccttc ctgttgctta tcacggcaga
     1681 gcaagttctg ttgtattaag cggtactgat atcattcgtc caaaaggtca gactaaagct
     1741 gacgatgctg aaactccttc atttggacca agcaggttat tagactttga acttgagatg
     1801 ggatttttta ttggtaccgg taatgaactt ggaaatccta tcagaattga aaatgcggaa
     1861 gaacatatat ttggaatggt attagttaat gactggagtg ctcgtgatat tcagaaatgg
     1921 gaatatgttc cgctcggacc ttttctcgct aaaaattttg ctacttcgat ttctccatgg
     1981 atcgttacaa tggaagcact tgaaccattt aaaacaaaaa gtgaaataca ggatcctgag
     2041 cctctcccct atttaaaaag agtaaaagaa aattcatatg atataaaatt agacgttatt
     2101 ttgaaaactc ccggatcaga aaaagaagag atcctgtccg aatcaaattt taaatatatg
     2161 tactgggata tgtgtcagca gcttgcacat cacactgtca caggatgtaa tatgcgtccg
     2221 ggtgatcttc ttgcatcagg cactataagc ggaccggaaa aaggaactta cggaagtcta
     2281 cttgaaatca catggcgcgg atctgaaccg gtgaaacttt caaacggaga tgaactaaaa
     2341 tttctcagag acggcgatga acttatcatg agaggatatt gtgaaaaaga tggattaaga
     2401 attggctttg gagaagtaac aggaaaaatt ttaccatcta attagatatg aacattatgc
     2461 taaaatgtgt ttctgtttta ttaattgtaa tttctgtttc gtcgtgtagt gacaattctc
     2521 aaaacggaaa taatattgta tatgacgaga actttcaata tccatattca ctaaattcct
     2581 tctggtatta ttctacaaag aatgcagttg taaatattcg tcctgattct ctcaaacgtt
     2641 tatataaagg agatactgct tcaggttatg gattttcttc ttttgtcaat gatacagtta
     2701 tagaaaacaa aaatgtcaga atactgaaaa atgaatacag tctggatgga cacagctcag
     2761 tcgtaagaga atattttgaa cagactgatt caggattgat acgattggca agtttatcaa
     2821 atggttctaa tctaagtcca ttcagaggaa atattaaata ttatattaac ggtaattatt
     2881 ttaacagttt aaatgaaatt tcattcagat ataataatga tcttatatct aatgacagca
     2941 ttgtattaat agataaccct ccattaaaaa caattaaata tcctttagaa ttaaattctg
     3001 aatgggaatt aattaataat ggatttgtca gaattcagaa aaaatataca gggtttgaaa
     3061 acattgacat ctccggaaaa aattttcatt gtatgaagat aagcagaagt tattatttca
     3121 gcggaagttt acctgatgaa aattttattg tgacggatta tttttctgca gaaggaattg
     3181 taagaaggga tctgttaata aaaaatataa atgtagtaaa tgaattaggt aatcttttgg
     3241 gctatgttga tctgtctgaa aaaactacag taaatttatt ttctattcca taacttttta
     3301 aaatatttta aaaacttaat acagaaattt ggataatgaa aaagtaagac ttacacaaat
     3361 ggttacctct gcaggctgcg cagcaaaaat atttcctgat atactttcca aaacattaaa
     3421 agaaattaca tgggatacga atgaaaatgt acttgttggc tttgaaggaa aagatgatgc
     3481 cggcgtttat aaaatttctg ataatactgc actgatccag actacagatt tttttacacc
     3541 tgtagtggat gatccttata cattcggaca gattgccgct gccaatgcac tcagtgatgt
     3601 gtatgctatg ggcggtgaac cgatatgcgc actgaacatt gttgcatttc ctcaaaaaga
     3661 agatctcaat attctgaaag aaattctcag aggcggtgct gataaaatga aagagtcagg
     3721 agctgtcatt ataggcggac attctataga tattaccaat atagtttacg gacttgcagt
     3781 tacagggaaa ataaatccaa atgaaattaa aggtaacgat tctgcaaagc cgggtgatat
     3841 actcattctt acaaaagcac ttggaacagg aatactaaat aatgtaatta aatttgcgca
     3901 gcttgaagat aagatttatc agcagcttat ttcttctatg actagactta ataaagatgc
     3961 tgcagaatgt atgaagcttt gtcatgctaa tggctgcact gatattacag gatttggtct
     4021 ggcaggtcat tcaatgcaga tggctgttgc aagtaaagct gtattgaaat tttttattaa
     4081 tcaactccct gtattacccg gagtcgagca tgcgataggc gaaaaactat tgacacgagg
     4141 tgacaagtct aacagaaatt atactaaaga taatacaatt agcgaaggtg aaataaatat
     4201 aataaaagaa catttacttt atgatcctca gacatctggc ggacttctca tcactgttcc
     4261 taaagaacat tcaaatatac ttcttctcga acttcacaaa cgcggagata tttatgcagc
     4321 tattgtcggt gaagtattgg aaagcactga tcagtataaa gaaggtagtg taatttttaa
     4381 ttatgaatga tcctgatttg aattggctgg agttgatttg aaagataaag aactttcaga
     4441 taaagaattt attgaatcag taatttctga tttttcaaaa gataaaggtg atgatctctc
     4501 cgtaaaggat ctgttaaaga attatgaaat taaatatatc gatattaatg agcttcattc
     4561 agatctcaag aatattccca ataagatttt attaattgat gcaagatcgg aaaaagaatt
     4621 ttctaatgca tcaatcccct ttgcaaaaaa ttttcctgta ttaacaaatg aagagcgaca
     4681 ttatgtcgga attatttaca aaaaatattc acagtctgca gcaataaaac ttgccttaaa
     4741 atatgccgtt ccaaaaacag aggatctaaa gaaatttata gaaacaaatg gcactcaaaa
     4801 taaaaaaata attgtgcact gctggagagg cggagggaga agtgcttatc tttcgaaaat
     4861 gatctatgat ctgggttttg aagtcgaagt actttcggga ggaataaagt cattcaggaa
     4921 agaagtaaat aaattttttg agctggataa atttcctttt gagttactgg aaatatcagg
     4981 gctgacagga tgcggtaaga ctgaattact gaattctgtt tcacaaagac ttccagtaat
     5041 tgatctggaa tttgccgcta agcatttttc aagtctgctc ggacatattc cttttgaaat
     5101 taaaggtgta aaaaaagtta ctggccagtc tgaatttgaa aataatattt ataatcagat
     5161 cagattaaat aattccgaac actgcgctta tgacaattta aattatccat ttctgataga
     5221 aagtgaaagc agacgagtag gaaaatttac tgtgcctgaa gttttataca aatctatgga
     5281 gtctgcatca tcaataaaat taacaggatc catgagcagc agagtgaaca gaattgttgg
     5341 tgattatttt tcagataaag aaaaagggat tgagctaatg acagatcttt tcaaaacaaa
     5401 aggtaatttt tttaaacaac agcttagtaa taaaatattt gatgagctat taatattgtt
     5461 agaaaaagga gatgtttatg attttactga aattatgata aaagattatt acgataaaag
     5521 ataccgcgaa aagaaaaaaa ttcctatttc tgaaattaat tcagataata tggaatccgc
     5581 ttcggatgta ttaatagaac tatataagaa aaaatatatt ttttaagcct cccaaccatt
     5641 tccatccaaa acgttttagc ataaaatatg atgttatcac tttctactgt tcttttttga
     5701 gccatcaaaa aagaaccaaa aaaatcgtcc cgattacccg tgatgataaa ttgccggaaa
     5761 ttatttcgca atgtctacaa aaaaataaac tcctcatccg tttgaaaatc actaatggat
     5821 tcgtcaaaca gtatattttt gcttaacgcc attgcacaga atttctaacg tcaatttatc
     5881 acaggcggtt gatctaacta ggtttaaaac agaattaaac tgtgagatag attttatata
     5941 aaataataaa ctacattatt aaatttatat aaaaactaat tattcaatta taaaatgttt
     6001 ttgacacaaa ataccttcta ctctcaagac aatacttcaa ttgaaattta cttcaaattt
     6061 atagagtgat cgttaacttt taattatgaa aatcaaatac ctggaccaga tcatttgaaa
     6121 aagatttcct cataatctct tcctgatttt ttgtaaactt tgagaaagac ccttcagccg
     6181 gactggagct ttcttttcta cttacacaat aaagcttctg ccagtttgca agatcattct
     6241 gtaataatat tgctatgaaa ttccatgcac ccatattttt tggttcttcc tgaacccaga
     6301 tcacacggtc agcattttta tatgaagcaa gaatttctgt cataacatca gtcttgtatg
     6361 gataatattg ttcgagcctt acaatcgcag tatcgaaatg ttcattctcc ttctgatatt
     6421 ttctcaggtc ataataaatt ttaccactgc agattatcac actctttatg tcagatttat
     6481 tttcaggaac atcatcaatt atttctataa acttaccgga tgtaaaatct tcttttatgg
     6541 atttagcttc aggcagtctc aagagacttt ttggagtcat tattacaaga ggtcgtctct
     6601 ttctgtgttt ggtctgcttt ctaagcaaat gaaaatattg tgcggatgta gtaaggttac
     6661 aaactatcat attgtcatca gcgcaaattg tcaaaaatct ttccagtctt gcacttgaat
     6721 gttccggtcc ctgaccttcc tgcgcatgag gcagcagcat aacaatatta ttaggcagtc
     6781 cccattttgt ataagagctt gcaataaaat tatcaatgat cacctgagcc gcatttacaa
     6841 aatctccaaa ctgagcttcc cataatacaa gtgtgatcgg atctgcagtg gaatagccat
     6901 attcaaatcc aagaacggct gcttcggaaa gtaaactgtc aagaggttcg atcttcgctt
     6961 tttcaggagc gacattattt aatggaatta tctcatctcc gttttcagca tcagttagta
     7021 taatatgtct ttgtgaaaaa gtacctcttg agctgtcctg cccgctgagc cttacaggga
     7081 taccttcctg caaaagactt ccataagcta gtgactctgc aaatgcccag tctatttcta
     7141 tatccttatc aagaaattct cttcttgatt caataaattt tttaagctta ggatgaatat
     7201 gaaaatcttc cggtatagtt gtgatcgcag ttacgatctc attcagttta tctataggaa
     7261 cgaatgtatc aaaatttgat tcgactgcaa gtatctcttc ttttgtaaca gcaagtggtc
     7321 tgtcagcttt aaacttctga actgattcat gactttcact catcttgtca tagatcttct
     7381 tttcagaaat ttcaatctct tcaggagtta tgactttttc tgataaaagt ttttttgtat
     7441 aaatctcttt tacagatgga tgacttctga tagtcttata catgatcggc tgtgtgaatg
     7501 caggttcatc tgcttcatta tgtccgagtc ttctgtatcc aaaaacatca ataaaaacat
     7561 cttcattaaa tttcattctg aattcaagtg caagttttgt aacaagcata gtagcttcag
     7621 gatcatcacc gttcacatga aaaatcggtg cctgtatcat cttgccaaca tcagttgcat
     7681 aaacagaaga tcttgcatct ataggagaag ttgtaaatcc gatctgatta tttataatta
     7741 tatgaattgt gcctcctgtt ttatatccat gtagttgaga aagattcaaa gtctctgcga
     7801 caattccttc tcctgccgca gcagcatctc catgaatcag cacaggaata attttatttc
     7861 tttcaagatc attcatcctc atttgtttag ctctgacaat tccttcgact actggattta
     7921 caaattccaa gtgagaagga tttgatgcaa ccgatactct gatcttttta tcatgaaaag
     7981 tttcatactc tcctgatgct ccgagatgat attttacatc acctgaaccc tgggaagata
     8041 tagggtcaat ataaccttca aattcagaaa aaatagttga catggatttc ccgataatat
     8101 ttgcgagcac atttagtctt cctctgtgag ccatgcccag tacaagttct tcgacggaat
     8161 aatctgcagc agaagaaaga agatgatcta aagttgcaat aatagtttca gaaccttcca
     8221 gtgaaaatct tttgtgtcca atgtattttt tatcaataaa cttttcaaaa ttctctgctt
     8281 cagataattt aaaaagtatg tgcttctttt cttcattaga aaatgaaggt aaattttttg
     8341 taggttccat tttatcctga agccatttct tttgttcagg atcctgaata tgtctgtact
     8401 caactccgat cttgtcgcag tatgtttgtt gcaggatatc aagtattgtt ctcaatgttg
     8461 ctgtacgcag accgccaagt ccgtcagtta taaattctct gtcataatcc caaacagtaa
     8521 atccgtaatt cgacggatca agctccggat gatactgtac tttgagatac agcggatcga
     8581 gatttgcaag cagatgacct cttacacgaa acatatttat taattgaatc gcctttgcct
     8641 gtctttcaat ttcttccaga ttatcaattc ttccaaggtc ctcggcagtc ttatctgaaa
     8701 accattttac aggtgtcatt ggaattccca ggtcttcaaa tatttcttca aaaaaattat
     8761 tttcacctct tagaagtccc gacaattctt tcagaaataa tcctgactct gctccctgta
     8821 ttattctgtg atcatatgta cttgtcatat tcatgatctt gctgatccct atagtagtaa
     8881 taatctcact tgttactgcc tgatattccg ccggataatc aattgcacct gtagcaataa
     8941 tcgcaccctg accaatcatt aatctcggag tcgatgcaac agtcccgatc gttcccggat
     9001 tcgtaagtgt aattgtcgtt ccctgaaaat ctgagatctc aattttattt tttcttgatc
     9061 tgtcaatgat atcattataa gctttgaaga attgacagaa atccatttga tttgctttct
     9121 taatatttgg aactataagc gatctgctgc cgtccttttt ttcaagatct actgcaagtc
     9181 ccaaattgat atctgtcttt tttacaagat tcggatcacc gt
//
LOCUS       DAORVQ010000131         9138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4148_length_9138_cov_4.740945, whole genome shotgun sequence.
ACCESSION   DAORVQ010000131 DAORVQ010000000
VERSION     DAORVQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4148_length_9138_cov_4.740945"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="PLX80_10340"
     CDS             <1..1349
                     /locus_tag="PLX80_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21228.1"
                     /translation="KYRPEFNNENLEKELVWKKLFPEKNYNYGIMKNIIHDLTGLSEK
                     FILLEKYSGESFRCELDLIEAGNSRNLKKFTSGKLDQFEKKIISEADPNKYSSINGFL
                     TVAGGFYDTKSSFIHEYNLKQDREEYLRLASDYSLFNFLVRSFKLLHNTFANEVHGNR
                     PVRKTLLEKFFVKLKEHSILEDLLINENENKDQDKISKIVMCFHLMYKAVTSDRDIAE
                     YYKFKSYLRENIDLFSGHEIKNLNNCRNTCAILLKISDSEVAKESLDWIKFLMEKNII
                     LLRSGLMYNTTMLNAVNYSVRLKEISFAEEFLNRYSGNLPAESRENTTNYCMAMIHFG
                     KGNFGKSLEYLSRISDEKLIRKYFVKRLYLKIYYELNDYESFIYAFDTFSHFKKRNKL
                     TNETRAMAFNNFGSNIRSLFKLRNSFEKFESIKLRKETGDQIWFIEKIDEIEKVII"
     gene            1368..2864
                     /locus_tag="PLX80_10345"
     CDS             1368..2864
                     /locus_tag="PLX80_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21229.1"
                     /translation="MHKSSLLEIIRTFTPKEIIKFEDFVNSPYFNKNKNVIKLFAEVI
                     KYRPEFNNENLEKELVWNKLFPEKNYNYGIMKNIIHDLNKLCESFLSEEIYNAKENQK
                     SNDFLESIIDRDLTDYFENKYAAYEKAFKKNFEKKNIRSTKDYYHQQKLLTERYEAFL
                     HNYSPQKKDSIKIIPSCEYLIYTFLIDSFKMMHKVIGHSLQYNHPLNENILYNHLKNL
                     EENNTIEMILKYTEAKPGNDHLILKCYYSMFKALFFNDDPDKFYEFKKNISVVTNILP
                     KSEIRDLYFTSMTCLTNLKVTLNNFSSEYFDIILLCYKSKVLLNKDGSISPHLFFSIV
                     NMACSQNELTFAENFIEEYSPKIPFDFRDAQFNYAMSGLFFKKGEFHKSLEYLSKIQN
                     EEKSMKYYIKNRQITIYFELNDRESFEYAFDSLKHYIRKNKLTNESRILTLTKYCEYI
                     KAMFKLREKYNSYDLDLLKNEIEKNKVSSKKWLLRKLEEIESENKKNR"
     gene            complement(3008..5086)
                     /locus_tag="PLX80_10350"
     CDS             complement(3008..5086)
                     /locus_tag="PLX80_10350"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21230.1"
                     /translation="MKIKIIYTAFLLFLVFNQNSFSQSGWFTQSQEYPSLLSSVFKDQ
                     NTGWIVGSQGLIYNTTNGGEDWIVQQSGTTQDLNSVIYTSDNTCWIAGNGGILLKSTD
                     YGQSWIKQSNINNFYIKSISFNNANTGWMCGSYGLIYKTTNSGQNWIQIPSNSNFVLN
                     KIIFTDDNTGWTISTQTFQGELNKTTNGGNTWFTVEYRQYSDIFFLDNNTGWKITYSE
                     VYKTTNGGTNWVSTNLRLFGSNYLHIMFSDANNGYISGSNGIIIATTNGGNSWEEKTN
                     IMGIIFNSIYFANSNTGWASGYYGIYKSTNGGNNWSSQKCGSANALSSVDAVSNNLAW
                     AVGSYGTILKTSNGGDCWMPQISGTSSRLQSVMFVNENTGWTCGDYDILKTINGGDNW
                     DNQFKDSSEYLHSVFFVNENTGWLSGFNMYKTTNGGNVWDIQTQSNGGFASLFFINEN
                     TGWTVENYINILKTTNSGNSWTKQSFTPGSGIGSVYFTDENSGWAVGSNAYKTTDGGD
                     NWYIQSLPVSQTYLSYVYFINNETGWITVQSGLIMKTTNNGNDWVIQPSDTNYFQSIS
                     FSDQNTGWIVGFGKYNNSLILKTVSGGTVGVSQIENSIPEIFSLSQNYPNPFNPTTKI
                     NFSIPVNGLVSVKIYDALGKEVLTLVNEHKPAGNYSAEFNGANLSSGVYFYRMEASEF
                     VDVKRMMLIK"
     gene            complement(5292..7475)
                     /locus_tag="PLX80_10355"
     CDS             complement(5292..7475)
                     /locus_tag="PLX80_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21231.1"
                     /translation="MKISKTFSFAVALCLFLTFSVSEMLKAQGFNSVTTADGINVIAV
                     GAGDAQGNNVYRSYNSGTSWAGYSIPGVVLKSVSSYNNDVWISGINGNIYKTQKNNSP
                     INTITTGITSSVNSINFISETLGFFCGDNGIVYKSIDGGLTWTLINTGIPSVKLNSVS
                     FLDEQKGIVVGDNGTAYVTANGGTSWSLENTGSTNNLLKVKYLADGIAVTGVNGTLIL
                     KPNASTWESVDTRVRTDIMGLTASDIDNIHVCGGGGFIRNNTNGNSRFYNFEVNPMMA
                     NLSDITYFNSQTGYAVSSLNNAIIKTTNGGILWTLTAGTSVSFQWENKLSSSGGIGNN
                     LCPHPTDKNSMFVVYGSKVYVSRNRGENWTQIATVSGGGSAHSFYVSPLDTNVWMCAI
                     TSSPDRVTRTTDYGVTWTTSISRNFSNYGQPLEMDQNNPSTYYFAPDGGGFYKSVDDG
                     VTFTEISNNYPFRSPCDVMVMWDSSDVIFIGDGVTGSGQAQIFKSVNKGVNWSLVKTA
                     SSSEIPSMCNTVFDQGLMYATEWSGSNIYKSSDYGSNWNTSHSTGFSGWASGFCVEDP
                     TMTLTGNYGSQSSLSTNSGSTWNTINGMSGAGAGMWVEDRGYLLSMQTSSLWKLKITY
                     SVATSVNENIISTNVPDNFSLEQNYPNPFNPTTNIKFAIPVSGNVTLKVFNQLGKEVE
                     TLTDGFKNAGSYELNFNAANLSSGVYFYKLETAGKIETKKMLLVK"
     gene            7894..8940
                     /locus_tag="PLX80_10360"
     CDS             7894..8940
                     /locus_tag="PLX80_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21232.1"
                     /translation="MNKIILIFFMSVFAFINKADSQNKKVLFEEFTNASCAPCALNNP
                     ALKAFTDSKGDTIISIKYHTNFPGFDPMYNLNPVQVEERRGGYYADVNAVPWLKGDGN
                     MFPDIWPFTQANFDAAFNTRKVIVPPLTISITDIRIAGDSVETTVTVNNTQNLPAGDY
                     KLRIMGVEKVINYTTPPGNNGESTFEDVFRIGVPDMNGVQLPTAAGVYFVTFKYKIQP
                     EWVDSNMVTTAFVQNDATGKEVINCNVASDVPTGIVNNNTNIPAKFNLYQNYPNPFNP
                     STTIKFDIPENSFVKLSIYNSIGKEVAVLINEMLIQGTYDYNFNASGLTSGLYFMRIS
                     SGNFQDTKKLMLLK"
BASE COUNT         3153 a         1537 c         1396 g         3052 t
ORIGIN      
        1 ttaagtatag gccggaattt aataatgaga atcttgagaa agaacttgta tggaagaagt
       61 tatttccgga gaaaaattat aattatggta ttatgaaaaa tatcattcat gatctgacag
      121 gattaagtga aaaatttatt ttgctggaga aatattccgg agaatcgttc aggtgtgaat
      181 tagatctcat agaagccgga aacagcagga acttaaagaa atttacttca ggtaagttgg
      241 atcaattcga aaaaaagata ataagtgaag ctgacccaaa caaatattca agtattaatg
      301 gatttttaac agtcgctggc ggattttatg atactaagtc aagctttatt cacgaatata
      361 atctcaaaca ggacagagaa gaatatctca ggctcgcttc ggattattca ttatttaatt
      421 ttcttgtacg ttcttttaag ttattacata atactttcgc aaacgaggta catggaaacc
      481 gtcctgtaag aaagacactt ctagaaaaat tctttgtgaa actcaaagaa cattctattc
      541 tcgaagatct tctgataaat gaaaatgaaa ataaagatca ggataaaata tcaaagatcg
      601 taatgtgctt tcatttaatg tataaggcag tgacttcaga ccgtgacata gctgaatatt
      661 ataaattcaa gtcttatctc agggaaaata ttgatctctt ttcaggacat gaaattaaaa
      721 acctgaacaa ctgcagaaat acgtgtgcta tcctgcttaa aatttctgat tcagaagtag
      781 caaaagaatc acttgattgg atcaaattct taatggaaaa aaacattatt cttctaagaa
      841 gcggattaat gtataacacc acaatgttga atgctgtgaa ttattcagtc agactaaaag
      901 agatcagctt tgctgaagag ttccttaata gatattcggg aaatcttcct gctgaatctc
      961 gtgaaaatac tacaaactat tgcatggcta tgattcattt tgggaaagga aatttcggga
     1021 aatctctgga atacctttcc aggatcagtg atgaaaaact tatacgcaaa tattttgtga
     1081 aaagacttta tctgaagatc tattatgaac tcaatgatta tgaatctttt atttatgcat
     1141 ttgatacatt ttcgcatttc aaaaaaagaa acaaacttac aaatgaaaca agagcaatgg
     1201 cgtttaataa tttcggcagt aatataagaa gtctttttaa actccgtaat tcttttgaaa
     1261 agtttgaatc aataaaactc agaaaagaga ccggcgatca aatctggttt atcgaaaaga
     1321 tagacgaaat agaaaaagtt ataatttaaa agttgtaatt tgaaattatg cataaaagct
     1381 cattactcga gatcatccga acattcactc caaaagaaat aatcaaattc gaagattttg
     1441 ttaattctcc ttattttaat aaaaataaaa atgtaattaa attatttgct gaagtaatta
     1501 agtataggcc ggaatttaat aatgagaatc ttgagaaaga acttgtatgg aataagttat
     1561 ttcctgagaa gaattataat tatggtatta tgaaaaatat cattcatgat ctgaataaac
     1621 tatgtgaatc atttctttct gaggaaattt ataatgcaaa ggaaaatcaa aaaagtaatg
     1681 attttcttga atcaataatt gacagggatt taactgatta ttttgaaaat aaatatgcag
     1741 catatgaaaa agcatttaaa aagaattttg aaaagaagaa tattagatca actaaagatt
     1801 attatcatca gcagaaacta ttgacagaaa gatatgaagc ttttttgcat aattatagtc
     1861 cacagaaaaa ggacagtatt aaaataatcc catcatgcga atatcttatt tatacttttc
     1921 ttatagatag tttcaaaatg atgcacaaag tgatcggtca cagtcttcag tataatcatc
     1981 ctctaaatga aaatatttta tataatcatt taaagaatct tgaagagaac aatacaattg
     2041 aaatgatttt aaaatataca gaagcaaagc cgggtaatga tcatcttatt ctaaagtgtt
     2101 attacagcat gtttaaagca ctttttttta atgatgatcc tgataaattt tatgagttca
     2161 aaaaaaatat ttccgttgta acaaatattc ttcctaaatc cgaaattcgg gatttatatt
     2221 tcacttcaat gacctgcctg actaatctca aagtaaccct aaataatttc agcagtgaat
     2281 attttgatat tatacttcta tgctataaga gtaaggtctt gcttaacaaa gacggttcca
     2341 ttagccctca tttatttttt tcaattgtaa atatggcttg ttctcaaaat gaacttacat
     2401 ttgcggaaaa tttcatagaa gaatactcac ctaaaattcc ttttgatttt cgtgatgcac
     2461 aatttaatta tgcaatgtcc ggattatttt ttaaaaaagg agaatttcac aaatcacttg
     2521 aatatctatc aaagattcag aatgaagaaa aaagcatgaa gtattatatt aagaaccgtc
     2581 agattactat ttatttcgaa ctgaatgacc gcgaatcttt tgaatatgct tttgactctt
     2641 taaagcatta tatcaggaaa aataaattaa caaatgaatc aagaatattg acactaacaa
     2701 agtattgcga atatataaaa gctatgttta agctgaggga aaaatataat tcctatgatt
     2761 tagatttatt aaaaaatgaa attgagaaaa ataaagtatc aagtaaaaaa tggcttctga
     2821 gaaaacttga agaaatagaa tctgaaaata aaaaaaaccg gtagatttct ctaccggctt
     2881 taatttcaac tatgaaattc taattacaaa taacattaat aaatctaaaa tcagtctatt
     2941 cccccacttg taattaattg ttaagatctg ttaaccaaac gggtaggcaa atccgaaatc
     3001 aaaatcgtta cttgatcagc atcatcctct tcacatcaac aaactcactt gcttccatcc
     3061 tgtagaaata aactccgctg gaaagatttg ccccgttaaa ttcagccgaa taatttcctg
     3121 ccggcttgtg ttcgttaaca agtgttaaga cttcttttcc aagcgcatca tatattttca
     3181 cacttaccaa tccattcaca ggaattgaaa agttaatttt tgtagtcgga ttaaatgggt
     3241 taggatagtt ttgtgacagt gagaaaattt ccggtataga attttcaatt tgagaaacac
     3301 ctactgttcc tcctgaaacc gtttttagaa ttaaactatt attatatttt ccaaatccaa
     3361 caatccagcc agtattttga tctgaaaaac taatagattg aaaataattt gtgtcggaag
     3421 gttgaatcac ccaatcattg ccattgttgg ttgttttcat aatcaatcca ctttgaaccg
     3481 tgatccatcc ggtttcatta ttaataaaat atacatatga taaataagtt tgacttacag
     3541 gaagagactg aatataccaa ttatcgccac catcagttgt cttatatgca ttgcttccta
     3601 ctgcccatcc cgaattttca tctgtaaaat aaacagatcc tattccgctt ccgggagtaa
     3661 aagattgctt agtccagcta tttccactgt ttgtagtttt cagaatatta atataatttt
     3721 caactgtcca gccggtattt tcattaataa aaaataatga agcaaaccca ccattagatt
     3781 gagtctgaat atcccataca tttcctccgt tggttgtttt gtacatatta aaacctgata
     3841 accagccggt attttcattt acgaaaaaaa ccgaatgtaa atattctgat gaatctttaa
     3901 attgattatc ccaattatct ccaccattaa ttgtttttaa tatatcataa tctccgcagg
     3961 tccagcctgt attctcattg acaaacatta ctgactgcaa ccgggatgat gtaccgctaa
     4021 tctgaggcat ccagcaatct cctccgtttg atgtcttgag aattgttcca taagacccta
     4081 cagcccaggc taaattattt gatacagcat ctacagaaga caaagcattt gctgatccac
     4141 acttttggct gctccagtta ttgccaccat ttgttgattt atatattccg taatatccgg
     4201 atgcccatcc tgtattactg tttgcaaaat aaatactatt gaaaatgatt cccataatat
     4261 ttgttttttc ttcccagcta tttccaccgt tagttgtagc tataataata ccgtttgatc
     4321 cggagatgta accattattg gcatccgaaa acataatgtg aagataatta ctaccgaata
     4381 atcttagatt ggtactaacc cagttagttc ctccgttagt agttttataa acttctgaat
     4441 atgtaatttt ccatccggta ttattatcaa gaaaaaaaat atcactgtat tgtctatatt
     4501 caactgtaaa ccaagtatta cctccgtttg tagtcttatt caattcacct tgaaaagttt
     4561 gagtgctaat agtccatccg gtattatcat cagtaaaaat tattttattc aaaacaaaat
     4621 ttgaattact tggaatttgt atccagttct gaccactatt tgttgtttta tatattaatc
     4681 cgtaactacc gcacatccag cctgtatttg cattattaaa tgaaattgat ttgatataaa
     4741 aattattaat attagattgt ttaatccagc tttgtccgta gtcagttgat ttaagaagaa
     4801 ttccaccgtt accggcaatc cagcatgtat tgtcagatgt gtaaataact gaattaagat
     4861 cttgtgttgt accgctttgc tgaactatcc agtcttctcc accattcgtt gtattataaa
     4921 ttaacccctg acttccgact atccagcctg tattctgatc cttaaatact gaagataata
     4981 aacttggata ttcctgactt tgtgtaaacc agcctgattg agaaaaggag ttttgattaa
     5041 aaaccaaaaa taaaaggaag gcagtataaa taatttttat tttcataatt ctttattatt
     5101 taagatttaa taaaattagt taattatgat aatatcaaac caaatttact tcatgaattt
     5161 atgtcttaaa atgcattaat tttaacacca atgttaagat ttgttaatct gacttctttt
     5221 tctaatttaa aataaaaaac ccggtacaga aacctatacc gggctattaa aattaactaa
     5281 agctgaattt tttattttac cagaagcatt ttctttgtct cgatctttcc tgctgtttct
     5341 aatttataga aataaacacc tgaagaaaga ttcgcagcat taaaatttaa ttcataactg
     5401 cctgcatttt taaatccatc tgtcaaagtc tctacttctt taccaagctg attgaatact
     5461 ttcaatgtta catttcctga aacaggaatt gcaaatttta tattagtagt aggattgaaa
     5521 gggtttggat aattttgctc aagcgaaaaa ttatcaggta cgtttgtcga aattatattt
     5581 tcatttactg aagtagcgac gctgtaagtt atttttagtt tccagagtga acttgtctgc
     5641 atagaaagca gataacctct gtcttcaacc cacattcctg cacctgctcc gctcattccg
     5701 tttatcgtat tccatgttga tccgctgttt gtgctaagtg aagactgcga accataattt
     5761 cctgtaagag tcattgtagg atcttcaacg caaaaacctg aagcccatcc actgaatcct
     5821 gtgctgtggc tagtattcca gttcgaaccg taatctgaag atttgtaaat atttgatcct
     5881 gaccactctg ttgcatacat taacccttga tcaaaaactg tattacacat tgaaggtatt
     5941 tcgcttgatg atgctgtctt aacaagcgac cagttcacac ctttatttac agatttaaat
     6001 atctgtgcct gtccagaacc tgtaactccg tctccaataa atataacgtc agagctgtcc
     6061 cacattacca ttacatcgca tggacttctg aatggataat tattactgat ctctgtaaat
     6121 gtaactccgt catcaactga tttataaaat ccgccgccgt ccggtgcaaa ataataagtg
     6181 cttggattat tctgatccat ctctaatggc tgaccataat tactgaaatt tcttgaaatg
     6241 gaagtagtcc atgtcactcc gtagtctgta gttctggtaa ctctgtcagg gcttgaagtt
     6301 attgcgcaca tccaaacgtt tgtgtcaaga ggacttacat aaaaagaatg agctgatccg
     6361 ccacctgaaa ctgttgcaat ttgagtccag ttttctcctc ggtttctgct gacatacact
     6421 tttgatccgt acacaacaaa catcgaattc ttatcagttg gatgtggaca aagattattt
     6481 cctattccac cacttgatga taatttattt tcccattgaa aggaaactga agttccggct
     6541 gtaagtgtcc acagtattcc gccgttagtt gttttaatta ttgcattgtt caaactgctg
     6601 acagcatatc ctgtctgact gttaaaataa gtaatgtcag atagattagc catcataggg
     6661 ttgacctcaa agttataaaa tcttgaattt ccatttgtat tatttctgat gaatccgccg
     6721 ccgccgcaaa catgaatgtt atcaatatct gaagctgtaa gacccataat atctgttctg
     6781 actctagtgt caacagactc ccaagttgaa gcattcggtt ttaagatcaa agtaccgtta
     6841 acaccggtaa cagctatacc gtcagctaaa tattttactt ttaaaagatt attggttgat
     6901 cctgtatttt caagactcca tgaagttccg ccatttgcag tcacataagc tgttccatta
     6961 tcgccgacta ctattccttt ttgttcatca agaaaactta ctgaattcaa tttaacagat
     7021 ggaattcctg tattaattaa agtccatgtc agaccaccgt caatagattt ataaacaatt
     7081 ccgttatcgc cgcaaaaaaa gccgagtgtt tccgaaatga aatttattga atttacagat
     7141 gaagtaatac ctgtcgttat agtattaatt ggtgaattat ttttttgtgt tttgtaaata
     7201 tttccgttta taccggagat ccatacatca ttattatatg atgatacact tttcaataca
     7261 actccgggta ttgaatatcc agcccaactg gtacctgagt tatatgaacg gtaaacatta
     7321 ttaccctgtg catctcctgc acctactgct atgacgttaa taccgtcagc agttgtaaca
     7381 ctgttaaaac cctgcgcctt taacatttct gaaacagaaa atgtcaaaaa aagacacaaa
     7441 gcaacagcaa aagaaaatgt tttactgatt ttcatttaat taattttagt tgttaaataa
     7501 gtatgtgcaa aattatggta tttcatttgc aattgcaatt tagtttatag aaaaaattgt
     7561 atacaaattc ttttgcttgc ctcaaagaca cagaaactct aagaaataat attgttattc
     7621 gatatatttt aaaaatataa atcatcattc atttttaata gattatcaaa aaatataaca
     7681 gttaagtttt actccagctc ctgaaggatc aggaaaaagt aatttttaag atttcaaatt
     7741 aagaacttac aatcttagag ccattgagtc ttaaagcctt ataggcaaag tcagtaatgc
     7801 tatagtaaac tttaatttga ttaattaaaa acttttttag tttttatgaa ttacttaact
     7861 ttatttaatt ccaattaaat taaattttaa aaaatgaata aaattattct gatttttttt
     7921 atgtcagtat ttgcctttat aaataaagca gattcacaga acaagaaagt actttttgaa
     7981 gaattcacaa atgcatcctg tgcaccttgt gcattaaaca atcccgcttt aaaagcattt
     8041 acagattcta aaggagatac aattatatca attaagtatc ataccaattt ccccggcttt
     8101 gatccgatgt ataaccttaa tccagtccag gtcgaagaaa gacgcggtgg atattatgca
     8161 gatgtaaatg cagtcccatg gctgaaaggt gacgggaata tgtttccgga tatttggcct
     8221 tttacacagg caaattttga tgcagctttt aacaccagaa aagttatcgt tcccccactt
     8281 acaatttcaa ttacagacat aagaattgcc ggtgattctg ttgaaacaac tgttactgtt
     8341 aacaacacac aaaacttacc tgcaggagat tataaactca gaataatggg tgtggagaaa
     8401 gttataaatt atacgactcc tcccggaaat aacggtgaaa gcacttttga agatgtattc
     8461 agaattggag ttcctgatat gaatggtgtt caactgccaa ctgctgccgg agtttatttc
     8521 gttactttta aatataagat tcagcctgaa tgggtagatt cgaatatggt cactactgca
     8581 tttgttcaga atgatgccac tggtaaggaa gtcataaatt gtaacgttgc aagtgatgtc
     8641 ccaacaggta ttgtaaacaa taatactaac attcctgcaa aattcaatct ttatcagaat
     8701 tatccgaatc cgtttaatcc ttctactact atcaagtttg acatacctga aaattcattt
     8761 gtaaaattaa gcatctataa cagcataggc aaagaagttg ctgtactaat aaatgaaatg
     8821 cttattcagg gaacgtatga ttataacttt aacgcatcag gtcttacatc gggtttatat
     8881 tttatgagaa tatcttcagg gaattttcag gatacaaaaa aattaatgct gcttaaataa
     8941 tttaattttc agttcaattt aactcattct gctgtttatt cagaatgagt ttttaattta
     9001 aaatatatgt tgaaagaata tttagaacgg ataggatatc aggatgatat aaatacatcg
     9061 aaagaagttt tgtttcagat acaagccgct cacatgctgc acattccatt tgagaatctt
     9121 gacatacact taaagaat
//
LOCUS       DAORVQ010000132         9093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4179_length_9093_cov_4.566608, whole genome shotgun sequence.
ACCESSION   DAORVQ010000132 DAORVQ010000000
VERSION     DAORVQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4179_length_9093_cov_4.566608"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1510
                     /gene="lat"
                     /locus_tag="PLX80_10365"
     CDS             122..1510
                     /gene="lat"
                     /locus_tag="PLX80_10365"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HQY21233.1"
                     /translation="MTNLTAENTAKDVQNENSQNGKFNSVNISPENVQTELKKHMLVD
                     GFDITIDIEKSEGAYLYDSKHGKNYLDFFTFVASNPLGMNHPKLNNEKFIKEIGKVAV
                     NKPSLSDIYTQTQAEFVDTFFEIAVPDYFKHSFFIEGGALAVENTLKAAIDWKVRKNF
                     KKGYTEEKGHQIIHFRQAFHGRSGYTMSLTNTDPNKVMYYPKFNWPRVDNPKITFPLN
                     EKNLADVIAAEDKTVEQIKAALNANKDDIAAIILEPIQGEGGDNQFRKEFFLKLRELC
                     DENEMMLILDEVQTGIALTGKMWAHMHYVQPDLIAFGKKTQVCGVLSTGRIDEIEDNV
                     FRTPSRINSTWGGNMVDMKRFTKILQIIKEEDLVKNCEVMGEYLQNKLSELANKHTEK
                     ISNTRGLGLFCAFDAADGKMRNQIQSGAMDNQLLILGSGEKSIRFRPPVNIGTEDIDK
                     GIAILDKVINSI"
     gene            1562..2026
                     /locus_tag="PLX80_10370"
     CDS             1562..2026
                     /locus_tag="PLX80_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQY21234.1"
                     /translation="MTFEDIKKTFIAYLKDNAHRITNERFLILNAALNMEEHFDADEL
                     YLNMKNDYLNVSRATVYKTLELMSECDILTKHNFKGDRTRYETKIGRKPHYHLICVNC
                     NKIIEFEYPQIEKIQDKICKENNFKMVDHTLQVFAKCNDVGSCKNNSGSNTN"
     gene            2040..2285
                     /locus_tag="PLX80_10375"
     CDS             2040..2285
                     /locus_tag="PLX80_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HQY21235.1"
                     /translation="MNYNNSEDQMKTAAEMKIGEKAIIEDIDNEHPSSHRLMEIGFTP
                     GQEIELLNKSIFNDPISFSIRGTIIAIRKNEASSIII"
     gene            2282..4324
                     /gene="feoB"
                     /locus_tag="PLX80_10380"
     CDS             2282..4324
                     /gene="feoB"
                     /locus_tag="PLX80_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQY21236.1"
                     /translation="MTLQVTQNKIPLIAITGQPNSGKTTLLNQLSGKKFKTVNYPGST
                     VEYSISKILSKYSVNANILDSPGIISLIPSSPDEEISVNTLFEHPSHGTPDIITVTVD
                     ASQLSRHLYLVRQLINSGFKVVIVLTMLDLLHKKGLDISDKKLSEILKSPVVRVDSNS
                     GKGVDVFLTTVNKLITDLQTNKEINSVIKYAPDNKENLLKSYTEIEEIEKDVIFPVNK
                     SESNDKPDLNKINESLIVLNNRGTFQKPDEFTIKLDRIVLHKFWGLIIFFLTMLFMFT
                     SVFYLAAPLMDIVNDSFSFLSDNASQILGNNWFGNLISQGVISGVGSVLVFLPQILIL
                     FLILGLLEDSGFLARGAMLIDKPLSKIGLNGKSFVPMLSGFACAIPAIMAARTITNRK
                     ERMLTIFIIPLMSCSARLPVYALLIAFLIPQEEAFTGGLILTLIYIFSIMSSVIIASI
                     VNKFSGKLLGSKDDSSFILELPAYRKPKVSVAISNMFSNAKQYIFKAGPIILYLSMLL
                     WILTYFPNYDPVVNESGKGEDEIVLMKNSERIATSYASEIGYFIEPVMKPLGMDWRVG
                     VSLIATFAAREVFVSSLMLIFKVTDTGEDMQSTLLSAMRNATFENTDIKIFTTATTAG
                     LIVFFVFALQCLSTVAIAKKETGGWRIPVLQIISFTAIAYIFAFITVNGLRFFGIN"
     gene            complement(4325..5428)
                     /gene="ychF"
                     /locus_tag="PLX80_10385"
     CDS             complement(4325..5428)
                     /gene="ychF"
                     /locus_tag="PLX80_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQY21237.1"
                     /translation="MSFRCGIVGLPNVGKSTLFNAITSSQNAEAANYPFCTIDPNVGT
                     VVVPDQRVTDLVELFNPKKIVPATIEFVDIAGLVKGASKGEGLGNQFLSHIREVEAIV
                     HIVRCFDDDNIVHVEGKIDPKGDIEIIETELILKDIDTIDKRLDKAGKGLKSGDKKAK
                     MEVDLLNRLKDHLSGSRLAKYFTKELNEEESEIIRELHLLSDKPVLYVANVDDKNLNG
                     NQYSELVKSISEKENAEFLILSVQIESEISQLESKEEKTEFLKEMGLDMSGLDKLIKK
                     GYELLNLITFFTAGEKEVHAWTIPTNLKAPQAAGEIHTDFEKGFIRAEIIKCNDLIEL
                     KSESAVKEKGLMRVEGKEYIVADGDVVFFRFNV"
     gene            complement(5443..5925)
                     /locus_tag="PLX80_10390"
     CDS             complement(5443..5925)
                     /locus_tag="PLX80_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21238.1"
                     /translation="MITIDYYRFKHPGFQGFFMKIFGNILRSHEVGGKKTIFTGGTYL
                     VLAFLMCVIVFPKPVAVMSMLVLVFSDTFAAFSGKIFGKTKIGNKTLEGSIGFFISGI
                     IIVLLTPKITDTNSELIIATITLFLTTAFELIPLKIDDNFTIPMFFGITYLILFNLFL
                     "
     gene            complement(6128..8155)
                     /gene="ftsH"
                     /locus_tag="PLX80_10395"
     CDS             complement(6128..8155)
                     /gene="ftsH"
                     /locus_tag="PLX80_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQY21239.1"
                     /translation="MTNNKTPKKNNNSEEFNWNRVFKIVLGWSAILIGFFLIMVYTKG
                     SDGKFSELKYDQYLTFLNDKKIESAVIKKSDNNYEFLGKLKSPETITVGGRSVTIDKF
                     NVFLPYTNIDDNVVKTWTESGVQFNIEKDDGGWMAPLLGALPWILIIAFWIIIMRRMQ
                     SGGAGGGKNIFSFGKSKAKLLSETGTKVTFNDVAGADEAKEELSEIIEFLKTPAKFQK
                     LGGKIPRGVLLLGPPGTGKTLLARAVAGEAGVPFFSISGADFVEMFVGVGASRVRDLF
                     EQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLIEMDGFEQNNGVIIIA
                     ATNRPDILDPALLRPGRFDRQVVVDRPDVKGREGILKVHTRKTPLANDISIDIIAKGT
                     PGFSGADIANLVNEAALLAARRNSDNVTMNDFEEAKDKVMMGTARRSLIISDKEKKTT
                     AYHESGHVIVAKMIPESDPVHKVTIIPRGRALGVTTYLPIDEKHTYSKEYLEAMLAYA
                     MGGRAAEKLIFNEYTTGASNDIDRATSMARRMVCEFGMSEKLGPISYGQKQEEIFLGR
                     EINQHRDYSESTQILIDDEIKRIIQSGMDKAERLLNENIDKLHIMSDALLERETLDSF
                     EIDMIMRGEELPPYEKPEKNEELKNDGELSKGKKKKVEDDKFAPGDIALAN"
     gene            complement(8168..8701)
                     /gene="hpt"
                     /locus_tag="PLX80_10400"
     CDS             complement(8168..8701)
                     /gene="hpt"
                     /locus_tag="PLX80_10400"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQY21240.1"
                     /translation="MPRKDQSELKSFISKDKIQKRVKQIAKQINKDYKGKKPIFIGIL
                     NGSFIFLSDIIRELKIDCEIDFLKLSSYGDEKITSGQVRMLKELNCDIKDKDIIVVED
                     IVDSGLSVIFIKKLIEKHKPASLKFASLLFKKNVSNLNFKIDYIGFEIDNKFVVGYGL
                     DYAQKYRNLNAIYVLKS"
     gene            complement(8796..8993)
                     /gene="rpsU"
                     /locus_tag="PLX80_10405"
     CDS             complement(8796..8993)
                     /gene="rpsU"
                     /locus_tag="PLX80_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQY21241.1"
                     /translation="MLKVLVQDGESIDKALKRFKKKYEKSGLLKEFRRRMFFTKPSVE
                     KKMDKERAIRKNKRILAEENI"
BASE COUNT         2924 a         1614 c         1490 g         3065 t
ORIGIN      
        1 ctagtggtac aaaatatgga ttagctcctg taactgtaat aataggatat actgaataac
       61 ttactttgca aaattttcat ataaaacaat tctataaact aatactaaat aacagactac
      121 tatgacaaac ttaacagcgg aaaatacagc taaggatgta cagaatgaaa attctcagaa
      181 tgggaaattt aattccgtaa atatatctcc tgaaaatgta caaacagaac taaagaaaca
      241 catgctagtg gatggctttg atattacaat tgatattgaa aaatctgaag gcgcatatct
      301 ttatgattca aagcacggaa aaaattatct ggacttcttt acatttgttg catcgaatcc
      361 tcttggtatg aatcatccaa aactcaataa tgaaaagttt ataaaagaga ttggaaaagt
      421 agctgtgaat aaaccttccc tgtctgacat ttatacacaa actcaggcag aatttgtaga
      481 cacatttttt gaaattgctg ttccggatta tttcaaacat tcattcttta ttgaaggcgg
      541 agctcttgca gttgagaata cacttaaagc agccattgac tggaaagtga gaaagaattt
      601 taaaaaagga tatactgaag agaagggtca tcagataatt catttccgac aggcatttca
      661 cggcaggagc ggatatacaa tgtcgcttac aaataccgat ccgaataagg tgatgtatta
      721 tccaaaattt aactggccaa gagtagataa tccaaagatc acttttcctc tgaatgaaaa
      781 aaatcttgct gatgtaatag ctgcagaaga taaaactgtt gaacagatca aggctgcatt
      841 gaacgcaaat aaagatgaca ttgcagcgat tattcttgag ccgatacagg gagaaggtgg
      901 agataatcaa ttcagaaaag aatttttttt aaaactgaga gagctttgtg atgaaaatga
      961 aatgatgttg atacttgatg aagtacagac tggaattgca cttacaggaa agatgtgggc
     1021 acacatgcat tatgtacagc ctgacctgat tgcatttgga aagaagacgc aggtatgcgg
     1081 agtgctttca acaggaagga tagatgaaat tgaagataac gtattcagaa ctccaagcag
     1141 gatcaattcg acatggggtg gtaatatggt ggacatgaaa agatttacga aaattctaca
     1201 gatcataaag gaggaagatc ttgtaaaaaa ttgtgaagtc atgggagaat atctgcagaa
     1261 taaattaagt gaacttgcaa acaaacatac agagaaaatc agcaacacca gaggtctcgg
     1321 actattctgc gcatttgatg cagcagacgg aaagatgaga aatcaaattc agtccggagc
     1381 aatggataac cagcttctga ttttaggatc aggagaaaag tctatcagat tcagaccgcc
     1441 ggtgaatatc ggaactgaag acattgacaa aggaatagca attttagata aagttataaa
     1501 tagtatttaa aatgtaattt tcttatcttt attaagacaa attcttaata aggaaatcga
     1561 aatgacattt gaagatataa aaaaaacatt tatagcttat ctcaaagata acgctcatcg
     1621 catcacaaat gaaagatttt tgatactaaa cgctgctctg aatatggagg agcattttga
     1681 tgctgatgag ctttatctta atatgaagaa tgattatctc aatgtatcga gagcaactgt
     1741 ttataagact cttgaactta tgagcgaatg tgatatactt accaaacaca atttcaaagg
     1801 tgaccgcaca agatacgaaa ccaagatcgg cagaaaacct cactatcatt tgatctgtgt
     1861 aaattgcaac aagatcatcg agtttgaata cccgcagata gaaaaaattc aggataaaat
     1921 ttgcaaagag aataatttta aaatggtaga tcatactctt caggtttttg caaagtgcaa
     1981 tgatgtagga agttgtaaaa ataattcagg aagtaacaca aactaataat ttttataaat
     2041 tgaattataa taattcagaa gatcagatga agacagcagc ggaaatgaag attggtgaaa
     2101 aagcgatcat agaagatatt gacaatgaac atccatcatc acacagacta atggagattg
     2161 gatttacacc gggtcaagag attgagttgc tgaacaaatc tatatttaat gatccgatct
     2221 cgttttcgat ccgaggaaca attatagcaa tcagaaaaaa tgaagcttca tctattataa
     2281 tttgacacta caggttactc aaaataaaat ccctttaata gcaattacag gtcagccgaa
     2341 ttcaggaaag actacacttc ttaatcaact cagcggtaaa aaatttaaga cagtaaatta
     2401 tccgggctct acagttgaat attccatttc gaaaatatta tccaaatatt ctgtcaacgc
     2461 aaacattctt gattcacccg gtataataag tctaatcccc tcttctcctg atgaggaaat
     2521 ttccgttaat actttattcg aacatccaag tcacggtact cctgacataa ttacagtaac
     2581 agtagatgcc agccagcttt caagacattt gtatcttgta agacagctta ttaattctgg
     2641 atttaaagtt gtgatcgtac ttacaatgct ggatcttctt cataaaaaag gtttagatat
     2701 ttcagataaa aagctttcag aaattttgaa aagccctgta gtcagagttg attcgaactc
     2761 aggaaaaggt gtagatgttt ttcttactac cgtaaataaa ttaatcactg atcttcaaac
     2821 taataaagaa ataaattctg taatcaaata tgctcctgac aataaagaga atcttttaaa
     2881 atcttatacg gagatagagg aaattgaaaa agacgtaatt tttcctgtca ataaatctga
     2941 gtcaaatgac aaacctgatt taaataagat caatgaaagt ctgatcgtat taaataaccg
     3001 cggtacattt cagaaaccgg atgaattcac tataaaatta gacaggatag tccttcataa
     3061 attctgggga ctgatcatat tttttctgac gatgttgttt atgttcactt ctgtatttta
     3121 tctggcagca cctttaatgg atattgtgaa tgattcattt tcatttctat ctgataatgc
     3181 ttctcagata cttggaaata actggtttgg taatttaatt tcacagggag tgatcagcgg
     3241 tgtaggatct gtactagttt tcctgccgca gatcttgata ctttttttaa tactcggact
     3301 gctagaagat tcgggatttc ttgcaagagg tgcaatgctc attgacaaac cattatcaaa
     3361 aattggtctt aacggaaaat cttttgtgcc aatgctttcg ggatttgcct gtgctatacc
     3421 tgcaattatg gcagcaagga ctatcacaaa ccggaaagaa aggatgctga caatttttat
     3481 aattccttta atgagctgca gtgcgagact gccggtgtat gcattgctga ttgcttttct
     3541 tatcccgcag gaggaagcat ttactggagg actgatactg acacttattt acattttcag
     3601 tattatgagt tctgtaatta ttgcttccat agtaaataaa ttcagcggta aattacttgg
     3661 cagcaaagat gattcgtctt ttattttaga actccctgct tacagaaaac cgaaagtatc
     3721 agttgcaata tcgaatatgt tttcaaatgc aaagcagtat atttttaaag ccggtccgat
     3781 cattctgtat ctttcaatgt tgctttggat acttacctat tttcctaatt atgatccggt
     3841 tgttaatgaa tcagggaagg gagaggatga aattgtacta atgaaaaatt cagaaaggat
     3901 agcaacctca tatgcctctg agattggata ttttattgag cctgtgatga aacctctcgg
     3961 aatggactgg cgcgtgggag tttctctgat tgctacattt gctgcgagag aagtttttgt
     4021 aagttcactt atgctgatct tcaaagttac tgatacaggg gaagatatgc agagtacttt
     4081 gcttagtgct atgcgtaatg ctacttttga aaatacggat ataaaaattt ttacaactgc
     4141 aactacagcc ggtcttattg tattttttgt atttgcactt caatgtcttt ccacagtagc
     4201 aattgccaaa aaagaaacag gcggatggag gatacctgtt ttgcagataa tttcttttac
     4261 agcaatagca tatatatttg cttttataac tgtaaacggt ttacggttct ttgggataaa
     4321 ctgatcagac attaaatctg aaaaacacta catcgccgtc agctacaata tattcttttc
     4381 cctcgactct catcagccct ttttccttta cagcgctttc agatttcagc tctatcagat
     4441 cattgcattt aataatttca gctctgataa atcctttttc aaaatcagta tgtatctcac
     4501 ctgctgcctg aggagcttta agattagttg gaatggtcca tgcatgaact tccttttcac
     4561 cggcagtaaa aaatgtgatg agatttaaaa gttcgtatcc ttttttaata agcttgtcta
     4621 acccggacat atccagtccc atttctttca gaaactctgt cttctcttct ttcgattcaa
     4681 gctgggaaat ttcagattcg atctgaacag ataaaataag gaactcagca ttttcttttt
     4741 cagaaatact ttttactaat tccgaatact gatttccatt aagattttta tcatctacat
     4801 tggcaacgta aagaacaggt ttatctgaaa gtaaatgaag ctctctgatg atctctgact
     4861 cttcttcatt aagttctttt gtaaaatatt ttgcaagccg tgatcctgat aaatgatctt
     4921 tcaatctgtt cagcagatct acttccatct ttgctttttt atcgccgctc tttaaacctt
     4981 taccggcttt gtctaatctt ttatcaatag tatcaatatc tttaagaatg agttcagtct
     5041 caataatttc gatgtcccct ttcggatcta tcttgccttc gacatgaaca atattatcat
     5101 catcaaaaca tcggacaata tgaacaatag cttctacttc tctgatatga gaaaggaatt
     5161 gatttccgag accttcacct ttagacgcac ctttcacaag tccggctata tcaacaaatt
     5221 caatagtagc tggtacaatt ttcttgggat taaaaagttc gacaagatcc gtgactcttt
     5281 ggtcaggaac tacgactgtt ccaacatttg gatcaatggt acaaaaagga taattagcag
     5341 cttcagcatt ttgtgaagaa gtaatagcat taaacaaagt tgacttacct acatttggca
     5401 gaccaacaat tccgcatctg aatgacattt tataagttta tattataaga ataaattaaa
     5461 tagaattaaa tatgtgatac caaagaacat tgggatggta aagttatcat cgatctttaa
     5521 ggggatcaat tcgaaagctg ttgttaaaaa taatgtaatc gttgcaatta tcaactctga
     5581 attagtatca gtaattttag gagtcagtaa aacaatgatg attccggaga tgaaaaatcc
     5641 gatacttcct tcaagcgtct tatttccgat tttcgtttta ccaaatatct ttccgctgaa
     5701 tgcagcaaaa gtatcgctga aaactagcac cagcattgac ataacagcaa caggttttgg
     5761 aaatactatt acacacatta aaaaagctaa aacaagatat gtacctccgg taaaaattgt
     5821 tttttttcca ccgacctcat gagacctaag aatattacca aagatcttca taaagaatcc
     5881 ctgaaatccc ggatgtttga atctgtaata atcaatagtt atcatgagga ttgtctgtac
     5941 tgacagaaac aaaatgaaaa attctctgct cccaaaaaat atgtaagcaa taggaattat
     6001 cgatgagaaa aggtgaatgc cctttctaaa taattcattt ttaagatcgg tctttttaat
     6061 actttccaaa tgtcaaaaaa aaagcgagtt aaattctcgc tttttattat ataaattatt
     6121 ttgaagttta atttgcaagt gcaatgtcac caggagcgaa tttgtcatcc tcgacttttt
     6181 tctttttgcc ttttgaaagt tcaccgtcat tttttaactc ttcatttttt tcaggttttt
     6241 cataaggtgg taattcttca cctctcatta tcatatctat ttcaaatgaa tcaagagttt
     6301 ctctttctaa aagtgcatct gacattatat ggagtttgtc aatattctca tttagaagtc
     6361 tttctgcttt atccatacct gactgaatta ttcttttaat ttcatcatct atcagtatct
     6421 gggttgattc actgtaatct ctgtgctggt ttatctccct tcccagaaag atttcttcct
     6481 gtttctgacc gtaagaaatt ggtccgagct tttcactcat tccaaattca cagaccattc
     6541 ttcttgccat gctcgttgct ctgtcaatat cgttactggc acctgtagta tattcattga
     6601 atattaattt ctctgcagct ctacctccca ttgcatatgc aagcatagct tcaagatatt
     6661 ctttagagta agtatgcttt tcatctatcg gtagatatgt ggtcacacct aaagctcttc
     6721 ctcttgggat aatagttact ttatgaacgg gatcagactc agggatcatc tttgctacga
     6781 ttacgtgacc gctttcatga tatgcggttg tctttttttc tttatctgaa attattaaac
     6841 ttcttcttgc agttcccatc atcactttgt ctttggcttc ttcaaagtca ttcattgtta
     6901 cattatcaga attccttctg gctgctaata aagcagcttc attaactaag ttagcaatat
     6961 cagctccaga aaatccggga gttccttttg caataatgtc tatacttata tcatttgcta
     7021 aaggagtttt tcttgtgtga actttcagaa ttccttctct tccttttaca tcaggtctgt
     7081 caacaacaac ttgtctgtca aatctacccg gtcttaataa tgcaggatca agtatgtcag
     7141 gtctgttagt tgctgcaatt ataattacgc cgttattctg ttcgaatcca tccatctcaa
     7201 taagaagctg attcagagtt tgttctcttt catcatgtcc gcctccaagt cctgcacctc
     7261 tgtgtctacc cactgcatct atttcatcta taaaaattat acaaggggca tttttctttc
     7321 cctgttcaaa aagatctctt actctggaag ctccaacgcc tacaaacatt tccacaaaat
     7381 ctgcacctga tatagaaaag aatggaacac ctgcttcacc tgctacagct cttgccagca
     7441 aagttttacc ggttccggga ggacctaata aaagaacacc tcttggaatt tttccaccga
     7501 gcttttgaaa tttagcggga gtttttaaaa attctattat ctctgataac tcttctttgg
     7561 cttcatctgc gccagctaca tcattgaatg taacttttgt accagtctcg ctcagaagtt
     7621 ttgctttgga ttttccgaaa gaaaaaatat tctttccacc tccggctcca cctgactgca
     7681 ttcttctcat tatgattatc cagaatgcta taatcagtat ccatggcaat gctccaagca
     7741 atggtgccat ccatccgccg tcatcttttt caatattaaa ctgaactccg ctttccgtcc
     7801 aggtctttac gacattatca tcgatgtttg tataaggaag gaatacgtta aatttatcta
     7861 tagtaacaga tcttcctccg actgttatgg tttctggaga ttttaatttt ccaagaaatt
     7921 cataattatt gtcagatttt ttgattactg cactttcaat ttttttatca ttaaggaatg
     7981 ttaagtactg atcgtatttc aattcggaaa attttccgtc actgcctttg gtataaacca
     8041 tgatcagaaa aaagccgatg agaattgcac tccagcctaa tacgatctta aatactctgt
     8101 tccagttgaa ctcttcgctg ttattatttt ttttaggagt tttattgtta gtcatttttt
     8161 tttggattta actttttaac acatatatag catttaaatt cctgtatttc tgggcataat
     8221 caagtccgta acctactaca aatttgttgt caatctcaaa tccaatataa tcaattttga
     8281 aattcagatt ggaaacattt tttttaaaaa gcagacttgc gaatttaaga gaagcgggtt
     8341 tatgcttttc gatcaatttt tttataaaga tcacagaaag acctgaatct acaatatctt
     8401 ccactacgat tatatctttg tcctttatat cacaattaag ttctttcagc attcttactt
     8461 gacctgaagt gatcttttcg tctccatagc ttgaaagttt caaaaaatct atttcacaat
     8521 caatttttaa ctcacggata atatcggaga gaaatataaa ggaaccgttt aatataccaa
     8581 taaatatggg tttttttcct ttataatctt tgttgatttg ttttgcaatc tgtttaactc
     8641 ttttttgaat tttatctttt gaaataaaac tttttaattc actctgatct tttcggggca
     8701 aatttctgta attttttatt taggaaaaat ctttaaaatt aaaaaaaccc actgaatatt
     8761 tatacaatgg gttttattaa aaaaaaaatt gtcaattaaa tattctcttc ggcaagtatt
     8821 cttttattct ttctgatcgc tctttcttta tccatctttt tttctactga tggttttgta
     8881 aagaacatcc ttcttctgaa ttcttttaac aagcctgact tttcatactt ttttttgaat
     8941 cttttcagag ctttatctat agactcaccg tcctgtacca gtacttttaa caaattattt
     9001 acctcttttc ttttaaaaat tttataaata taattaattt cgatttttat tacaatgaaa
     9061 gtttcaataa gtttttaaaa atgtttttta tat
//
LOCUS       DAORVQ010000133         9093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4181_length_9093_cov_3.991370, whole genome shotgun sequence.
ACCESSION   DAORVQ010000133 DAORVQ010000000
VERSION     DAORVQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_9093_cov_3.991370"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLX80_10410"
     CDS             complement(<1..671)
                     /locus_tag="PLX80_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HQY21242.1"
                     /translation="MTYLNELFNLQKGEDDKGKTLESVKNNITFTGANLWILACAILV
                     ASIGLNVNSTAVIIGAMLISPLMGPIVGSGFALGIYDFALLRKSLRNLFIATIAGLIV
                     SFIYFYISPFKEAQSEILMRTEPNIYDVLIAFFGGLVGVIAVTRLEKGNPIPGVAIAT
                     ALIPPLCTAGYGLSIGDMKFFLGSIFLYSINCVFICIATFVIVKYLKYPSKEIDIGQR
                     KKVKY"
     gene            complement(836..1039)
                     /locus_tag="PLX80_10415"
     CDS             complement(836..1039)
                     /locus_tag="PLX80_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21243.1"
                     /translation="MKKRDENQPVVIAISENNIEEYIQKIESNGGKIAVPKFPVPSIG
                     LVLLFNDPDGNLHGKRQEDRALK"
     gene            complement(1271..2710)
                     /locus_tag="PLX80_10420"
     CDS             complement(1271..2710)
                     /locus_tag="PLX80_10420"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQY21244.1"
                     /translation="MSKSDIDHHEEDTHFFKDVCSYFDKAAGFTTHHAGLLNQIRQCN
                     CIYKFNFPLRLDNGDIEVLDAWRIEHSHHKNPVKGGIRYSLMVNEDEVKALAALMTYK
                     CAVVDVPFGGAKGGIKIDPKKYTERQLENITRRYTSELIKKNLIGPGIDVPAPDYGTG
                     EREMSWIADTYATFNTGQIDAFACVTGKPLSQHGIKGRREATGRGVFFGLREAVSIKE
                     DMDKIGLSTGMEGKTVVLQGLGNVGYYAGKYCQEAGSVLIAIAEYEGAIYDPKGLDLD
                     EVFHHRKTTGSILNFKNAINLSFTSEALELECDILIPAALENQITSANAGRIKAKIVG
                     EGANGPVTPEAEKIMLDKNILIIPDLYLNAGGVTVSYFEWLKNLSHVSFGRMDKRYEE
                     LANLNFVNVLDATSSIPLSKLQRDTLIRGASEKELVDSGLEETMMSAYNEIREIKMND
                     SKVDSLRTAAFISAINKIAISYANLGIFP"
     gene            3019..5121
                     /locus_tag="PLX80_10425"
     CDS             3019..5121
                     /locus_tag="PLX80_10425"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21245.1"
                     /translation="MSVYLFPKSILSQNSWYSINGSQSSTQDINGLDIVNSNVIYAVG
                     GGGYIYKSVDAGINWDKIVNNATSQYLWSVSFADEQTGWAVGYGGTIVKTVNGGTSWQ
                     IQNSGTYSALLDVQFLDQMTGWVIGHNGIILKTINGGMNWITQTSGSMENIFNIKFIN
                     SLTGYLSGDAGVFKTTNGGDSWTNNLNEPWVYSVDFVNANTGWAGVRYGKMFRTDDGG
                     ESWTEQNSGTSATYYSMKFLDENTGYAVGDYNTIIKTTNSGLNWVIENSGVENSFAKF
                     SRIDSYDLSGNVCFIVGFNSTILKTENGGTNWNNYFNFSISTIPTNIDLWSIFFTSND
                     VGWTAGSNGSIYKTTNGGLQWNQSAQGFVNDLLIDIFFTSDKHGWVVGHNGRILRTSN
                     NGLNWENQNSGTQDNLFNVFFYNNFVGWIAGDKGVLKTTNNGLNWEVCLSYDWMYSVY
                     FVDQNTGWAGGRFGKLIKTTDGGNTWTDQQGGTGATYYSIKFSDENNGWAVGDYNTII
                     RTTNGGATWIRQSSGIQSNSLKLSEIQIAVDDANTAYISTYENHILKTTNAGQDWYKS
                     TLPGYSSYNSIFFSSENIGYAAGFGASILKTENRGITGVVNENNILPESFSLNQNYPN
                     PFNPKTVIRYKITNSDNVSLKVYDILGNEVAELVNEKQIAGSYSVEFNGSGFSSGVYF
                     YRLESGNNFDTKRMLLIK"
     gene            5860..6147
                     /gene="rpsR"
                     /locus_tag="PLX80_10430"
     CDS             5860..6147
                     /gene="rpsR"
                     /locus_tag="PLX80_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQY21246.1"
                     /translation="MKRSTFKKSNYKLTQQITAKKNQPKKRDPFKEKGVKDYIDIFDA
                     RLLTRFLNEQGKLLPARITGATSKMQRKLNTAIKRARHLAIIPFVSEGTRY"
     gene            6231..7217
                     /locus_tag="PLX80_10435"
     CDS             6231..7217
                     /locus_tag="PLX80_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQY21247.1"
                     /translation="MQKILFTILINLFLFSGIACSQTSEDPLTLGTFRITKVVDGDTF
                     KFENMDGSSRLLGIDTEETFKTDDAERKTAEIEANWDEYYKFEKGDNKMPVKTNSPLG
                     YEAMVWAQDYLKNVEFARLEKEDENRSLDIFGRYLVYMFIEKDGKQINYNVEIVRLGY
                     SPYFNKYGNSKKYHNDFLEAQEYAMNNKLGIWDPEKKHYPDYEERIVWWNKRAEQLEN
                     FNSKYSGTENYFNLADDSDYKRLSENVGKEIVVFGNISDVLTKKFPYLLRIPHTKNET
                     FEVVVFEENINILDEIDIDSKKDYYTYVKGTLKEYKGRYQIELTDKEQIWVE"
     gene            complement(7231..8973)
                     /locus_tag="PLX80_10440"
     CDS             complement(7231..8973)
                     /locus_tag="PLX80_10440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQY21248.1"
                     /translation="MENNDPAKKQLVLNSLIEFSQLITSKLDLNYILNNILLSVMGKM
                     LISKGVVLLKTEGTDNIYKVKAAKGINLSLIDTFIEAKFPKLSVFSFNEIPENDVFVN
                     EHKFEYYFKIYFQNKYLGILCLGKKLNTSDLSNNDIIFIETMLNISASAIENTIKFEE
                     VSNLNNSLNNKVRQLNSLFELGKEFNSSFIDKENIIKLLNFSLLGNFGIKDFVILSRD
                     NKDKFVIVKDNKNLEIEKFDLYNLLSGDDIPKILKKTIVINEDSTIPFIKYLFEKDFE
                     LFIPTIINNKIDSIICLGKKLNKKPFSEEDIEFLESIMNLSLISIHNYFLFQEYLSKQ
                     KIDSELIIAREIQEALLPDKIPEIENYEIAGMNDPALQIGGDYFDFIKIAEDKLVIVI
                     ADVSGKGTPASLLMASIQSAVHSYLKLYEEGNFDFAKVTEKINELIYENTSSEKFITF
                     FWGILDSKENTFEYINAGHNPPQMLKGNKFIELTEGGFIIGILDIPMNYEIGKVEMGK
                     DDIIVFYTDGVTEALNIKDEEYGETNLKKVILNEKNNSPAVILDSIKNSVLEFSKNMP
                     QYDDITLIVLKKTT"
BASE COUNT         3083 a         1398 c         1465 g         3147 t
ORIGIN      
        1 ctgtatttta cttttttcct ttgtccaata tcaatttctt ttgagggata ttttaaatat
       61 ttaacaataa caaaagtggc aatacatata aagacacagt taatgctgta taaaaaaata
      121 gatcctaaaa aaaatttcat atcaccaata gatagtccgt aaccggctgt acaaagtggg
      181 ggtatcagag ctgtggcaat tgctacaccg ggtataggat ttcctttctc taatcttgtc
      241 actgctatta cacctacgag tccgccaaaa aatgcaatga gaacatcata aatatttggt
      301 tcagtcctca ttagaatttc agactgagct tctttaaaag gactgatata aaagtaaatg
      361 aaagaaacta tcaatccggc tattgtagca ataaataaat ttctaagtga ttttcttaat
      421 aatgcgaaat cataaatgcc tagtgcaaat cccgaaccta ctattggacc cattaacgga
      481 gatataagca tagcaccgat tataacagca gtagagttta catttaaccc aatcgaagcc
      541 acaagtatag cacatgcaag tatccaaagg ttggctcctg taaaagtaat attattttta
      601 acgctttcta aagttttacc tttatcgtct tcgccttttt gaagattaaa taattcgttt
      661 agatatgtca ttaacttgaa ttattgataa atatattctc gaaattttca aagtattatc
      721 cattcaatca tattactcaa aattgaataa aataaggaat gtctgaaagg gtaaagatta
      781 tataattaat ttgaaaaatt tataatcata aaaataattt aaataatcaa aatatttatt
      841 ttaaagcgcg gtcttcctgc cgtttcccgt gtaaatttcc gtcaggatca ttaaagagca
      901 gaaccaggcc aattgacggc acaggaaatt taggaacggc aatttttcct ccgttagatt
      961 ctatcttctg aatatattct tcaatattat tttcagaaat cgcaattaca acaggttgat
     1021 tctcatctcg ctttttcata atagctccgt ttattcactg tgtttcttca ttgcctgaaa
     1081 aggcagtcca ataatcatca gcaaattgtt catactcata tccaaacact ttttcgaaaa
     1141 aattcattaa cctcccgggt tcatcacttt gcatttcaaa atatataact ctgttattaa
     1201 tgatgtaaaa aaaatgaatt caaaaaaatt ttattgacga ataaacatta gactaaaatt
     1261 aattaatcca ttaaggaaat attcctaagt tagcatagga tattgcaatt ttatttatag
     1321 cacttatgaa agcagcagtt ctgagtgaat ctactttgct gtcattcatt ttaatttctc
     1381 tgatttcatt ataagcagac atcattgttt cttcgagtcc ggagtccacg agttcctttt
     1441 cacttgcacc tctgataaga gtatctctct gtagttttga taatggtatg ctgcttgtag
     1501 catcaagtac atttacaaaa tttagatttg ccagttcttc atatctttta tccattctac
     1561 caaaagatac atgtgaaaga ttttttaacc attcaaaata tgaaactgta acgccgcctg
     1621 cattaagata caagtctggg ataattaaaa tatttttgtc aagcatgatt ttttctgctt
     1681 caggagtaac aggtccgttt gcaccttcac caactatttt tgctttgatc cttcctgcat
     1741 tggcagaggt aatttgattt tcaagagcag cagggataag aatgtcacac tctaattcaa
     1801 gagcttcaga tgtgaaactt aggtttattg catttttgaa attgagaata cttcctgtag
     1861 tttttctgtg gtggaatact tcatcaagat ctaatccttt tggatcataa attgctcctt
     1921 catattctgc aattgcaatt aatactgacc cggcttcctg gcagtatttt ccggcataat
     1981 atccaacatt tccaagtcct tgtaatacca ctgtttttcc ttccattcct gttgaaagtc
     2041 caatcttatc catatcttct tttatactta cagcttcacg aagtccaaaa aatacacctc
     2101 ttccggtggc ttctcttctt ccttttattc cgtgctgact cagaggtttt cctgtaacgc
     2161 atgcaaatgc atcgatctgt cctgtattaa aagttgcata agtatctgct atccaggaca
     2221 tttctctttc acctgttcca taatccggag caggtacgtc aatacccgga ccgatcagat
     2281 tttttttgat cagctcagaa gtatatcttc gggttatatt ttcaagttgt ctttcagtgt
     2341 atttttttgg atcaatttta attcctcctt ttgctccgcc aaaaggaacg tcaacaacag
     2401 cacatttata agtcataagt gcagcaagag cttttacttc atcttcattt accatcaggc
     2461 tgtatctgat acctcctttt acaggattct tgtgatgaga atgttcgatc ctccatgcat
     2521 ctaaaacttc aatatctccg ttatccaatc tcaaaggaaa attgaatttg taaatacaat
     2581 tgcattgcct gatctgattc aaaagccctg catgatgcgt tgtgaatccg gcagctttgt
     2641 caaaataact gcagacatct ttaaagaaat gtgtgtcctc ttcgtgatga tcaatatcac
     2701 ttttgctcat aataaaaatt aaattagtta aaattttgta tggaaaaaaa tatgttaaaa
     2761 ctttgttttt ttaaagttaa atttttttat acaaacaaat ttttctgttg atatttattt
     2821 gaatatattg aaaaactttt tgagtaattc aaattcattt tcaaatggaa tttctgtccg
     2881 atttttaaaa ttataaatgt tcttggtata ttgtaattta agattaataa ttgtaaattt
     2941 gataaaaaag gtaacccccc acggaggtaa ataactatga gaatcaaaat ttgtttaacg
     3001 gttttatcat ttgttttttt gtcagtttat ctttttccaa aatcaattct atctcaaaat
     3061 tcctggtatt ccatcaatgg ttctcaaagc agcactcaag atattaacgg acttgatatt
     3121 gtaaattcaa atgttatcta tgctgtaggc ggaggtggat atatttataa gtcagtcgat
     3181 gccggtatta attgggataa gattgtgaat aatgctacaa gtcaatatct atggtcagta
     3241 tcatttgcag atgagcaaac aggctgggct gtagggtatg gagggactat tgttaagaca
     3301 gtaaacggag gtacatcatg gcagattcag aattcaggta cttactcagc tttgcttgat
     3361 gtacagtttc ttgatcaaat gacaggatgg gtaatcggac ataacggcat tatattaaaa
     3421 actataaatg gcggaatgaa ctggatcact cagacatcag gtagtatgga aaatattttt
     3481 aatatcaaat ttataaactc tctgacagga tatctctcag gtgatgctgg agtttttaaa
     3541 actacaaatg gtggagatag ctggacaaat aatttaaatg aaccctgggt ttattcagtg
     3601 gattttgtaa acgcaaacac cggctgggca ggtgtcagat atggaaaaat gttcaggact
     3661 gatgacggag gcgaatcctg gactgaacaa aacagcggta ccagcgcaac ttattattcg
     3721 atgaaatttt tggatgaaaa tactggctat gcagtaggag attataatac aattataaaa
     3781 actacaaaca gtggattaaa ctgggtaata gaaaacagtg gagttgaaaa cagttttgca
     3841 aaattcagca ggatagatag ttatgattta tcaggcaatg tctgttttat agtaggattt
     3901 aatagtacta tattaaagac tgaaaacggt ggtactaact ggaataatta ttttaatttt
     3961 tcaatctcca ccattccaac aaatatagat ctttggtcta tattctttac aagtaatgat
     4021 gttggctgga ctgccggcag taatggatca atatataaga ctacaaatgg cggattgcag
     4081 tggaatcaat cagctcaggg atttgtaaat gatctactga tcgatatttt ttttacaagt
     4141 gataaacatg gctgggtagt aggacataat ggtagaatat taagaacttc aaataacgga
     4201 cttaattggg aaaatcaaaa ctctggcaca caagataatt tgttcaatgt ttttttctat
     4261 aataattttg ttggatggat tgcaggtgac aaaggtgttt taaaaactac aaacaacgga
     4321 ttaaactggg aggtatgttt aagttatgat tggatgtatt ctgtttattt tgttgatcaa
     4381 aataccggtt gggcaggtgg cagatttggt aaattgataa aaacaactga tggcggaaat
     4441 acatggacgg atcagcaagg cggtacaggt gctacatatt attcaataaa attttccgat
     4501 gaaaataacg gatgggcagt cggtgattat aatacaatta taagaactac aaatggcgga
     4561 gcaacatgga taaggcaaag cagtggaatt caaagcaata gtttgaaatt aagtgaaatt
     4621 caaattgcag ttgatgatgc taacacagca tacatttcta cttatgaaaa tcatattttg
     4681 aaaacaacca atgcaggtca ggactggtat aagtcaactt tacccggata ctcgagttac
     4741 aattctattt tcttttcatc tgaaaatata ggatatgctg caggctttgg tgcttcaatt
     4801 cttaagaccg aaaacagagg tattaccggt gtagtaaatg aaaataatat tttacctgaa
     4861 tcattttcac taaatcagaa ttatccaaat ccatttaatc caaaaacagt tatcaggtat
     4921 aagatcacaa attctgacaa tgttagttta aaggtttatg atattcttgg aaatgaagta
     4981 gcagagctgg taaatgaaaa gcaaattgcc ggcagctatt ctgttgaatt taacggcagc
     5041 ggtttttcaa gcggggttta tttttacaga ctcgaatcag gaaataattt tgatacaaag
     5101 cggatgctgt tgatcaaata gttctgaaat ttaattaaaa aatttaattg gtatgagatt
     5161 aattcttata ccaatttttt tatgaatcat ttagagaatt gattgaccta agttactatc
     5221 aaaggaatga tcatatgcca ttcatcagcg ctggatcttg caactactgc aacgacaggt
     5281 tctgaaacac tcattttaaa tacttcgtga gggtcacctg attcaataaa aataaaatca
     5341 ccagtattat tttcaatgac ttttttttac ataccggtcc gtattcgtgt ctgacatttc
     5401 cttccggtat acaagcatta tttcaatggt cgcgacattc atggagagtt cttttgaacc
     5461 gacatttttg atgacattcc agttttatat tgaatgccat ttcagtcccg acatttgccg
     5521 cttcctctat taacattaat gccgtcacct ttttctacat tatgagtaag attgatctct
     5581 tcttttgaca taatattatt tttgtttcaa tgattataaa gttttaattt taaaagtata
     5641 tacaagattc tttcatattt actatgaatt ttaaatctta tgaaggagat ttcatttttt
     5701 aaattcagat cactgacagg atatgtaaag gttcttatta aattatttat gttaataaat
     5761 atggatataa taaatttttc taaaataccc taaattagaa ttgtaattcc tcattaaaat
     5821 caataaatta gacgaccttt ttaaaaaata aatttttcaa tgaaaagaag tactttcaaa
     5881 aaatcaaatt ataagttaac acagcagata actgccaaaa agaatcagcc taaaaaaaga
     5941 gatccgttca aagaaaaagg cgtaaaagat tatattgata tattcgatgc tagactactt
     6001 accagatttc taaatgagca gggaaagtta ttacctgcaa ggatcaccgg agctacttcc
     6061 aaaatgcaga gaaaacttaa tactgcaatt aaaagagcca gacatcttgc catcattcca
     6121 tttgttagtg aaggtacgcg atattaaatt atacgatctt ttaaaataat ttttatttaa
     6181 tttttataaa ctctttcgct ttggcgttag agttttttta atttaagaat atgcaaaaaa
     6241 tattatttac aattctcatc aacctatttc ttttttcagg aatagcatgc tcacaaactt
     6301 cagaagatcc tcttactttg ggaacattta gaataaccaa agtagtagat ggagatactt
     6361 ttaagtttga gaatatggat ggatcttcca gattgctggg cattgataca gaagaaacat
     6421 ttaaaactga cgacgccgaa aggaaaactg cagagatcga agcaaactgg gatgaatatt
     6481 ataaatttga aaaaggtgac aataaaatgc ctgttaaaac aaattcacct ctcggatatg
     6541 aagcaatggt atgggcgcag gattatctta aaaatgttga gtttgccaga ctagaaaagg
     6601 aagatgaaaa cagatcactg gatatttttg gtaggtatct tgtctatatg ttcatagaaa
     6661 aggacggtaa acaaataaat tataatgtcg agattgtcag acttggctac agtccttatt
     6721 ttaataaata tggtaacagt aaaaaatatc ataatgattt tctggaagct caggaatatg
     6781 ctatgaataa taaacttggc atctgggatc cggagaaaaa gcattatccc gattatgaag
     6841 aaagaattgt atggtggaat aaaagagctg agcagcttga aaactttaat tcaaaatatt
     6901 caggaactga aaattatttt aatctggctg atgatagtga ttataaaaga ttaagtgaaa
     6961 atgttggtaa agaaatagtt gtatttggaa atatcagtga cgtacttaca aaaaagtttc
     7021 catatctttt aaggattcct catactaaaa atgaaacttt tgaagtagtt gtatttgagg
     7081 aaaatataaa tattttagat gaaatcgata tagattctaa aaaggattat tacacttatg
     7141 taaaaggaac acttaaagaa tataaaggaa gatatcagat tgaacttact gacaaagaac
     7201 agatctgggt tgaataattt tatctggtaa ttatgttgtt ttctttaaaa cgattaaggt
     7261 aatgtcatca tactgaggca tgtttttact gaattcaaga acagaatttt ttattgaatc
     7321 caatatcact gcaggggaat tgtttttttc atttaatata actttcttta aatttgtctc
     7381 tccgtattct tcatctttta tgtttaaagc ttccgtgact ccgtcagtat aaaatacaat
     7441 aatatcatct tttcccattt caacttttcc tatttcgtaa ttcattggga tatctaatat
     7501 tccaataata aatccacctt cagtaagttc tataaattta ttgcccttca acatttgcgg
     7561 tggattgtga cctgcattaa tatattcaaa tgtattttct tttgagtcca gaatacccca
     7621 gaaaaaagtt atgaactttt cagatgaagt gttttcatat atcaattcat taattttttc
     7681 tgttactttg gcaaaatcaa aattcccttc ttcatataac tttaaataag aatgaacggc
     7741 ggattgaata cttgccatca ataaagacgc cggtgtacct ttaccggaaa catcagcaat
     7801 tacaataact aatttatctt cagcgatttt tatgaaatca aaataatcac caccaatctg
     7861 aagtgctgga tcgttcatac ctgctatttc gtaattttca atttcaggaa ttttatccgg
     7921 cagaagtgcc tcctgtattt ctctggcgat tattaattca ctatcgatct tttgtttact
     7981 tagatattcc tgaaacagga aataattgtg aattgagatt aatgacagat tcataataga
     8041 ttccaaaaac tcaatgtctt cttcagagaa aggtttttta ttaagttttt ttccaagaca
     8101 aattatgctg tcgattttat tattaataat ggtcggaata aataattcaa aatctttttc
     8161 aaaaagatat ttaataaaag gaatagtaga atcctcgttt attacaatag tttttttcag
     8221 aattttagga atatcatctc ccgagagtaa attatacaga tcgaattttt caatctcaag
     8281 atttttgttg tctttaacaa taacaaactt atctttattg tctctggata atatcacaaa
     8341 atcttttatt ccaaaattac ccagtaaaga aaaattaagt aattttataa tattttcttt
     8401 atcaataaag cttgagttaa attctttgcc gagctcaaat aatgaattga gttgtcttac
     8461 tttattgttt aatgagttgt taagattact tacttcttca aacttgattg tgttttcaat
     8521 agctgatgct gaaatattca gcattgtttc aataaaaatt atatcattat tagaaagatc
     8581 tgaagtgtta agtttttttc ctaaacaaag tattccaaga tatttattct gaaaatatat
     8641 tttgaaataa tattcaaatt tatgttcatt tacaaataca tcattctctg gaatttcatt
     8701 aaatgaaaaa acagataatt ttggaaattt ggcttcaata aatgtatcga tcaaagaaag
     8761 gttgattcct tttgcagctt ttactttata tatattatct gtaccctcag tctttaaaag
     8821 aacaacacct ttagagatga gcattttccc cattacactc aggagtatat tgtttaaaat
     8881 ataattcagg tcaagtttac tggtgatgag ctgagagaat tcaattaatg aatttagaac
     8941 gagttgtttc ttggcgggat cattattttc cattttaatt tgaattatta tgagttattt
     9001 aatttctcat aatattttta tcaaagattt tttattaaag taagccggtt cgaatcggga
     9061 tttaagtgaa ggaactcaat tttattcata aat
//
LOCUS       DAORVQ010000134         9082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4192_length_9082_cov_5.371441, whole genome shotgun sequence.
ACCESSION   DAORVQ010000134 DAORVQ010000000
VERSION     DAORVQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_9082_cov_5.371441"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLX80_10445"
     CDS             complement(<1..646)
                     /locus_tag="PLX80_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HQY21249.1"
                     /translation="MLVISSGMQKSGTAYFYNVLNDLLIESGAENVRKIKDEFSLGNI
                     LKDNNNNIDGISWKDLLKISVVSFKKGTFAVKTHYPNRRSFGILSNAGFLKIIYSYRD
                     PRDVILSVIDHGKKLKESGETGSFTKMIELDDAVRNVKSWINDFKKLSKKNNILKYSY
                     EDFIKDPENILSKVSEFLKIEFSTKSVQEVLLRYNSSNPDMPKGRLHFNKGIVDR"
     gene            801..1379
                     /locus_tag="PLX80_10450"
     CDS             801..1379
                     /locus_tag="PLX80_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY21250.1"
                     /translation="MSKQKNMNSEKISINLIPKNADPEVKFIKDALKGSQEAFKNLFD
                     ENVSKIFSVCLRFTADTDHAKEITQEVFISAWKKLSTFKFECRFSTWLHKIAVNQFLM
                     HERTQNRIFKKKEDFSKGQIKHESEKINDLRIDLQNAIIKLPDQARAVLIMYDIEGFM
                     HNEIAEILNIQTGTSKAHLHRARKLLREELKK"
     gene            1376..3148
                     /locus_tag="PLX80_10455"
     CDS             1376..3148
                     /locus_tag="PLX80_10455"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQY21251.1"
                     /translation="MTKENSKELCEEMELLIDDQLDGMISLSDKQKLENHIAVCDSCK
                     LYLKNTLETINQLDSLNSKKNIIQSADKEMIWDNISKNIDINEHAAAINTGETEPVNP
                     YNLFKKKYYWISGIAAVIAVFFIIYAVKNWNFTSGENSLEYSFGLPTYWKVVNLKGSP
                     QIGDSKISGSDSIKEGQWIITDSNSMAELIITDIGKVTIEPGSKVVFINGADSSGKIF
                     VEYGMINTQTINDKNKPFMVEMPSAVAENFGGSYSLKIDKKGDGMIFVKSGMVDIQSP
                     NKMAIVPAGNIVMTKNGVGVGTPFNEKASTKFRNALFDYDFGNCNNYCVNVLLDNAKM
                     TDAVSLVNILNGKPVDQEIEDEIYNKIVNYVPLPPNNNRDSIKYFDEKEFEIWIEDIQ
                     KRVETEMKSSMQQLEKNLSEYQNDMHEFYFDSAKFYFIPENFEAPEIPEVPKGVFSYK
                     YNHDSSYFDSEEFKKEMEKVKEEIKKENDFNKEELEREMKKVQEEIKKTNSINKDELN
                     KEMEELNKELEELNKNLEENIYFDSEEFKKEMEKVQEEIENSVKEYKKNYNNQNDHLK
                     EVPELPEEPEIENNDSGPDDDSDN"
     gene            3339..3971
                     /locus_tag="PLX80_10460"
     CDS             3339..3971
                     /locus_tag="PLX80_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HQY21252.1"
                     /translation="MSEISSYIKIFIAIMVLVNPLEGMSLFLSKTTGLDSDTRSKVIR
                     KTTLTVFIVLVTSLFFGKYVLTLFGIGIASFTVAGGIIIFLIALEMILGNSGSSSQQV
                     SADPGKSAMAIAVVPLGIPLLAGPGPISSVILYGSSSKGFVEDIVLVFIIVVVSIGVS
                     LSLNAAAKMEKRLKQNGVNILTKISGILVSAIAIEMIYSGIVKMFPQLHQ"
     gene            complement(4000..5097)
                     /locus_tag="PLX80_10465"
     CDS             complement(4000..5097)
                     /locus_tag="PLX80_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21253.1"
                     /translation="MRKSIDGSTRRLIELCVFYFIFYVITGVSVKYFLGSSSLGFPGM
                     QSMQYLVYSTTGGTLFALSIVLIRKWYKLQSNGYTSFLGLKIPEEFIYIIPSGICTAI
                     IIPTTTLMYSLPISVMVAMVIMRGSVIIISRIVDALQIRSGILKKKVYKEENIAVVFA
                     ILAVLINLFWQKDDSGFDFIYNAAAVIILSSYIVAYFIRIYIMNYYKNTRGKNIPQDN
                     KGFFAVEQIAAFVTLVVVGLVLFYSPQLFQWENKQINEFIGAIVNPAADWGWAIFAGT
                     AFGAVAFFSVFIFMYKGRTATFAGLVNRLTSLIAGTAATLIFYVFFGGKFPSNEDWIS
                     LIFILIAVAFITKAEKKRTAELKLTESENKQ"
     gene            complement(5119..5433)
                     /locus_tag="PLX80_10470"
     CDS             complement(5119..5433)
                     /locus_tag="PLX80_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21254.1"
                     /translation="MDILLISGLVLFTINYVTGWLLKFRLLSITKILHQIMFALLIIN
                     LLIILLFKVSGFYNISLCVLSLLMLFILPFGKKGSGYHITVSTIGFILYVILILNPLN
                     NF"
     gene            complement(5449..6225)
                     /locus_tag="PLX80_10475"
     CDS             complement(5449..6225)
                     /locus_tag="PLX80_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQY21255.1"
                     /translation="MDLFDAIKNRRTTNTAFADRKVSEEHIELLIRMASHSPSHFNSQ
                     PWRFIIIDDEKIIRDIAKIAGDSMTALMDDGRFWKQYRKYFRFSEEEMEQSKDGIHID
                     HLPVFLKPFAKSIFSEAGGKVISRFKVSRILGNDEEKLVATSPILFAITLKKDEYKKE
                     ELSGFYSVISLGAVIQTLWLVTTAIDMGMQFISTPGEIPAQWKALSDMLEVDENYELV
                     VVFRMGYVDKNIKRPVIDWKSSQRKSPGELAFRNKFGKKI"
     gene            complement(6303..7013)
                     /locus_tag="PLX80_10480"
     CDS             complement(6303..7013)
                     /locus_tag="PLX80_10480"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HQY21256.1"
                     /translation="MIIIFLISFHFLFFLNGCNSKSITKENIRSEKSDSLNSELSLKK
                     IELTEAQKKIINGARENLNENTEYDLTMGYYVLTYRDGEDTGSKVFPEGDLDPEIGVC
                     TDVIVRSLRYGGVTDLQKELNEDIISNRSDYPFSRWGNKKADPNIDHRRVMNLEVWFS
                     KYWETIETDTDESFLPGDIVVWDMNQDGASDHIGIVSDTFTGERFKVIHNHPDPGHIA
                     DEDKIFKWEISGHYRIRN"
     gene            complement(7056..7553)
                     /locus_tag="PLX80_10485"
     CDS             complement(7056..7553)
                     /locus_tag="PLX80_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HQY21257.1"
                     /translation="MISKEQIYNDVKEKLVSDKLEISGEDFERPWGGFFKIADNGLND
                     FLNIYFDGRELPGETKDLTLSPKILVVEPGKKLSWQCHERRSELWRVVRGPVGIYKSL
                     TDKQPDEMEIHNDSDVIEMELGIRHRLVGLEDWGIIAEIWIHRFKDNPSDEDDIERIS
                     DDFGR"
     gene            complement(7561..8766)
                     /gene="lhgO"
                     /locus_tag="PLX80_10490"
     CDS             complement(7561..8766)
                     /gene="lhgO"
                     /locus_tag="PLX80_10490"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HQY21258.1"
                     /translation="MKNFDVVIIGAGIIGLSTALQLIKNSQELKICILEKEDHISAHQ
                     TGHNSGVIHSGIYYKPGSLKAENCIKGYDLLLDFCNNYEIPYELCGKIIIAASEDQIP
                     AMKELQRRGEENGLKDIKEISQSEIKDYEPYASGVSALHIPQTGIVDYKSVSKKIFDL
                     ITDGNCKTFLSEKADRIEYSQNEVIIYTGKEKYSARCLVCCAGLYSDRLSQMCGLKID
                     YKIIPFRGEYYRLRDESKKFVKNLIYPVPDPKFPFLGVHFTKRMDGIIEAGPNAVLAF
                     GKESYKKSDFNFKESIDTLKYKGFREIAARYWKTGFYEYYRSFSKKEFVRSLQKLIPD
                     ITGDDLIPGGSGIRAQACTKDGRLIDDFLLAENENVINVCNAPSPAATSAFSIAELIS
                     EKTIKKLKT"
     gene            complement(8896..>9082)
                     /locus_tag="PLX80_10495"
     CDS             complement(8896..>9082)
                     /locus_tag="PLX80_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21259.1"
                     /translation="TESIDFGLTGGIGIIIPAGKKLGISIEIRDDLGLVNISKAPVYN
                     GGTLKNNTLNFIAGIVF"
BASE COUNT         3022 a         1580 c         1433 g         3047 t
ORIGIN      
        1 atctgtcaac aattccttta ttaaaatgaa gtctcccttt aggcatgtca ggatttgagg
       61 agttgtatct gagcaaaact tcttggacac ttttggtact aaattctatt ttcaggaatt
      121 ctgatacttt actcaatata ttttccggat cctttataaa atcttcataa ctgtatttta
      181 aaatgttatt tttttttgac aattttttaa agtcattgat ccaggactta acatttctta
      241 cagcatcatc taattctatc atcttagtga aactgccggt ctccccggat tcttttaact
      301 ttttcccgtg atcaatcacc gataaaatta catctctcgg atctctgtaa ctgtagatta
      361 tttttaaaaa accggcattt gataagattc cgaatgatct tctgtttggg taatgtgtct
      421 tgactgcaaa tgtacctttt ttgaaactta ctacggaaat tttgagtaag tccttccatg
      481 aaatgccgtc aatattatta ttattatcct ttagaatatt tcccagagaa aattcatctt
      541 tgatcttccg gacattttca gctcctgatt caattaaaag gtcattcaac acattataaa
      601 aatatgctgt accggacttt tgcatacctg atgaaattac aagcatacaa aataaattaa
      661 attgttcaat atattcaatt ctgctccttc aaagaaaatt taatttttaa aatcagtaaa
      721 atttatttaa attgttaaat taaatctaaa caaaattatt tttccatcta aaccaattga
      781 cttctcactg catctaatat atgagtaaac aaaaaaacat gaattcggaa aagatatcaa
      841 ttaatctaat cccaaaaaat gcagatcctg aagtaaaatt cattaaagac gcattaaaag
      901 gcagtcagga agctttcaaa aatttatttg acgaaaatgt ttcgaaaata ttttctgtct
      961 gtttgagatt tacagcagac actgaccatg ctaaagaaat tacacaggaa gtatttatca
     1021 gtgcctggaa gaaattatct acctttaaat ttgaatgcag attttctaca tggctgcata
     1081 aaattgctgt aaatcagttt ctaatgcatg aaagaactca aaacagaatt ttcaaaaaga
     1141 aggaagattt ttcaaaaggt cagataaagc atgagagtga aaaaattaat gatcttagga
     1201 tcgatctgca aaatgcaatt atcaaattac ctgatcaggc aagggcagtt ttgataatgt
     1261 atgatatcga aggatttatg cataatgaaa tagctgaaat actcaatatt caaacaggaa
     1321 catcaaaagc ccatttacac agggcaagaa aattattgag agaggagtta aaaaaatgac
     1381 aaaagaaaat agtaaagagt tgtgcgaaga aatggaattg cttatcgatg atcagcttga
     1441 tggtatgatc tcgctttccg ataagcaaaa actggaaaat catattgctg tttgtgacag
     1501 ttgtaaattg tatttaaaga atacacttga aacaattaat cagcttgatt cattgaattc
     1561 taaaaaaaat ataatccaat cagcagataa agaaatgatc tgggataata tttcaaaaaa
     1621 tatagatatc aatgaacatg ctgccgcaat aaataccggt gaaacagaac cggtaaaccc
     1681 gtataattta ttcaaaaaaa aatattactg gatctcagga atagcagcag tgatcgctgt
     1741 cttttttatt atatatgctg ttaaaaactg gaattttact tcaggtgaaa attcacttga
     1801 atattcattt ggtctgccga cttactggaa agtagttaat ctcaaaggtt cacctcagat
     1861 cggagattca aaaatttccg gctctgacag tattaaggaa ggacagtgga tcattactga
     1921 cagtaattcc atggctgaat tgatcattac agacattgga aaagttacta ttgagccggg
     1981 gtctaaagtt gtttttatta acggtgctga cagttcggga aagatctttg ttgaatatgg
     2041 aatgatcaat actcagacaa ttaatgataa aaataaaccg tttatggtgg aaatgccttc
     2101 tgcagttgct gaaaatttcg gcggttcata ttccttaaaa attgataaaa aaggagacgg
     2161 aatgatattt gttaaatcgg gtatggtaga tattcagtct ccaaataaga tggctattgt
     2221 gcctgcagga aatattgtca tgacaaaaaa tggtgttggt gtcggaactc cattcaacga
     2281 aaaagccagt acgaaattca gaaatgcact gtttgattat gattttggaa attgcaataa
     2341 ttattgtgta aatgttttgc ttgataatgc aaaaatgaca gacgctgttt ctcttgtgaa
     2401 tattctgaac gggaaacctg tagatcagga aatagaagat gagatctata ataaaatagt
     2461 aaattatgtg ccgcttcctc caaacaataa cagagacagt attaaatatt ttgatgaaaa
     2521 ggaatttgaa atatggatag aagatattca gaaaagagtt gagactgaaa tgaaaagttc
     2581 aatgcagcag cttgaaaaaa atctttctga atatcagaat gatatgcatg agttttattt
     2641 cgattcagct aaattttatt ttatccctga aaattttgaa gcacctgaaa tacctgaagt
     2701 tccaaaaggt gtatttagtt acaaatataa tcatgactct tcatattttg attctgaaga
     2761 gttcaaaaaa gaaatggaaa aagtaaagga agagatcaaa aaggaaaacg attttaataa
     2821 agaagagttg gagagggaaa tgaaaaaagt acaggaggag atcaaaaaga caaacagtat
     2881 aaataaagat gagctgaata aggaaatgga agagcttaat aaagagcttg aagaattaaa
     2941 taaaaatctt gaagagaata tttattttga cagtgaggaa tttaaaaaag aaatggaaaa
     3001 agtacaagaa gagattgaaa attcagtaaa agaatacaaa aaaaattata ataaccaaaa
     3061 tgatcattta aaagaagttc ctgaattacc tgaagaacct gaaatagaaa ataatgactc
     3121 tggtccggat gacgattcag ataactgacc tttgaaaatt tatgtatccg cagagtaacg
     3181 tgtgcttcgg attctgcgga tcagcattaa atatttaatt tagaaaaagt gaacagcatc
     3241 aaagcttttc actttttttt aacaatttta taattattgt aagtaagatg aattttttat
     3301 aatttaatgc atgaaaaatt tccctataaa aacttcttat gtctgaaatt tccagttaca
     3361 tcaaaatttt cattgcaata atggtgcttg taaatccatt ggaaggtatg tcactgttcc
     3421 tttctaaaac aacaggtctt gacagtgata caaggagcaa ggtcataaga aaaactacac
     3481 tgactgtttt tattgtgctt gtaacatcac ttttttttgg aaaatatgtc cttacacttt
     3541 ttggaattgg tatagcatct tttactgttg ccggcggaat tatcatattt ctgattgctc
     3601 tggaaatgat cctcggaaat tcaggttcgt cctcccagca agtatcagct gatccgggta
     3661 aaagtgcaat ggctattgca gtagtacctc ttgggatccc attgctggca ggtccgggac
     3721 cgatcagcag tgtaattctt tatggaagca gcagtaaggg atttgtagaa gatattgttc
     3781 ttgtttttat catcgtagtt gtcagcatag gtgtctcatt atctttaaat gcagccgcca
     3841 aaatggaaaa gagattgaaa caaaatggag taaatatact gactaaaatt tccggaatac
     3901 ttgtttcagc tattgctata gaaatgatat attcgggaat tgtaaaaatg tttccacagc
     3961 ttcatcaata attatttgac tgatctaaca attataattt tactgtttgt tctccgattc
     4021 agtaagtttt aattctgcag ttcttttctt ctcggctttg gttataaaag ccacagcaat
     4081 taaaataaat atcagcgaga tccagtcttc attacttgga aatttccctc cgaaaaatac
     4141 ataaaatatc aaagttgcag ccgttcctgc tataagtgat gtaagccggt tcacaagtcc
     4201 tgcaaaagtg gctgtccttc ctttatacat gaaaatgaac acagaaaaaa atgcaactgc
     4261 tccgaacgct gtcccagcaa aaattgccca gccccagtct gcggcaggat ttactattgc
     4321 tcctatgaat tcatttattt gtttattttc ccactgaaat aattgaggag aataaaacag
     4381 aactaatcca actacaacta aagtcacaaa tgctgcaatc tgttctacag caaaaaatcc
     4441 tttgttatcc tgagggatat ttttacccct ggtatttttg taatagttca ttatatagat
     4501 cctgataaag tatgcaacaa tataagatga aagtataatt actgcggctg cattgtatat
     4561 aaaatcaaac ccgctgtcgt ctttctgcca aaacagattt atcagaactg caagtattgc
     4621 aaagactaca gctatatttt cttccttata aactttcttt tttaaaatac ctgaacgaat
     4681 ctgaagagca tctacaattc tgctgatgat tattacactg cctctcataa ttaccatcgc
     4741 gaccattact gatattggga gcgagtacat aagtgttgtc gtagggatta taattgctgt
     4801 gcatataccg gatggaatta tataaataaa ttcttccggt atttttaaac cgagaaaaga
     4861 tgtataaccg tttgactgaa gtttatacca ctttcggatc aagacaatgg ataatgcaaa
     4921 taaagtaccg cctgttgtgc tgtaaacaag atactgcatg ctttgcatcc cggggaatcc
     4981 aagtgaactg ctgcctaaaa aatatttcac acttacacct gtgatcacat agaaaataaa
     5041 ataaaataca cataattcta ttagtcttct tgtgctgccg tcaatagatt ttctcaattc
     5101 aatttaattt aaatggcttt aaaaattatt tagaggattt agtattaata ttacatataa
     5161 aataaatcct attgtgctga ctgtaatatg atatccactt ccctttttcc caaatggaag
     5221 tatgaacagc attaataatg atagtacgca taatgaaata ttatagaatc ctgaaacttt
     5281 gaataacaat ataatcaaaa gattaataat aagtaaagca aacattatct gatgtaatat
     5341 ttttgtaatg gaaagtaatc tgaactttaa taaccagccc gtaacataat ttattgtaaa
     5401 taatacaagt cctgaaataa gcagaatgtc caaatttact tttaaaattt aaatcttttt
     5461 tccaaattta tttctgaaag caagttctcc gggacttttt ctttgtgatg atttccagtc
     5521 aatcacaggt ctcttaatat ttttatcaac ataacccatt ctgaatacaa ccaccaattc
     5581 ataattttca tcaacttcca acatatcaga aagtgctttc cattgtgcag gaatttcacc
     5641 cggtgtggaa atgaactgca tacccatatc tattgcagtt gtgaccagcc acaatgtttg
     5701 tatcactgca cctaatgaaa tgactgaata aaatcctgag agctcttctt ttttatattc
     5761 atctttcttt aaagtaattg caaataagat tggtgaagta gctacaagct tctcctcatc
     5821 attacctaaa attctcgata ctttgaacct ggaaattact ttcccgcctg cttcggaaaa
     5881 tattgacttc gcaaatggtt ttagaaatac cggtaaatga tctatatgta tcccgtcttt
     5941 agactgctcc atttcttctt cgctgaatct gaaatatttt cggtattgtt tccagaatct
     6001 tccgtcatcc ataagagctg tcattgaatc tcctgctatc tttgcaatat ccctgattat
     6061 tttttcatca tcaataataa taaatctcca tggctgggaa ttaaaatgac tgggtgaatg
     6121 agatgccatt ctgatcaaaa gttcaatatg ctcttccgaa actttcctgt ctgcaaaagc
     6181 agtattcgta gtccgccggt tctttatcgc gtcaaataga tccatacact aagatcagtt
     6241 tatttttttg aataacactt ttatataata atttcaaatt cataattact aatttttaat
     6301 tcctaattcc taattctata atgtcccgat atttcccatt taaaaatttt atcctcatca
     6361 gctatatgcc ccggatcagg atgattgtga ataactttaa atctctcgcc ggtaaaagtg
     6421 tcggacacta ttccgatatg atcacttgct ccgtcctgat tcatatccca tacaactata
     6481 tcaccgggta aaaaactttc atcagtatct gtctctattg tctcccagta ttttgaaaac
     6541 catacctcaa gattcataac tcttctgtga tcaatatttg gatcagcttt tttgtttccc
     6601 catcttgaaa atggataatc cgatctgtta gaaattatat cttcatttaa ttctttctgc
     6661 agatctgtaa caccgccgta tctcagcgat cggactatca catctgtaca gaccccaatc
     6721 tcgggatcca gatctccttc aggaaatact ttgcttcccg tatcttcacc atccctataa
     6781 gtcagtacat aataacccat ggtcagatca tattcagtat tttcatttaa attttcgcgc
     6841 gcaccattaa ttattttctt ctgtgcttcc gtaagttcga ttttctttaa agataattct
     6901 gaatttagag aatcagattt ttcactgcga atattttctt tggtaatact tttcgaatta
     6961 cagccgttca gaaaaaataa aaaatgaaat gaaattaaaa atattataat cattattttt
     7021 ctataatata ttctatatca aaaactgaag ttattttatc ttccaaaatc atctgatatt
     7081 ctttcgatat catcttcatc tgaggggtta tctttaaatc tgtgaatcca gatctcggca
     7141 attattcccc agtcttctaa tccgaccagt ctgtgtctga tacccagttc catttctata
     7201 acatcagagt cgttgtgaat ttccatttca tcaggctgtt tatcggttaa gcttttgtaa
     7261 atcccaaccg gtcctctgac cactctccag agttccgatc ttcgttcatg acactgccat
     7321 gataattttt tacccggttc gactacaagt atcttcggac tcagtgtcag gtcttttgtt
     7381 tccccgggaa gttcccttcc gtcaaaataa atattcagaa aatcattaag accgttatca
     7441 gcaattttaa aaaatccgcc ccatggtctt tcaaaatctt cacctgagat ttcaagctta
     7501 tcggatacca gtttttcctt cacatcattg tagatctgtt cttttgaaat catttttatt
     7561 ttatgttttt agttttttaa ttgtcttttc tgaaattaat tctgcgatgg aaaaagcaga
     7621 tgtagcagca ggggacggag cattgcatac attaataaca ttctcatttt cagcaagaag
     7681 aaaatcatca attaatctgc cgtcttttgt acatgcctgt gctcttatgc cgctgccgcc
     7741 gggtattaga tcatctcctg taatatccgg tatcagtttt tgcaatgatc tgacaaattc
     7801 tttttttgaa aatgaacggt aatattcata aaaacctgtt ttccagtatc ttgcagcaat
     7861 ttctctgaaa cctttatact ttaatgtatc aatagattct ttaaaattaa aatcactttt
     7921 tttgtaactt tcttttccaa atgcaaggac tgcgtttgga cctgcttcga tgataccgtc
     7981 cattcttttt gtaaagtgaa ccccgagaaa aggaaatttc ggatcgggga cagggtatat
     8041 aagatttttt acaaattttt tactctcatc tctcagcctg taatattctc ccctgaaagg
     8101 aattattttg taatctatct ttaaaccgca catttgtgaa agcctgtctg aataaagtcc
     8161 tgcgcagcat accagacatc ttgcggaata tttttctttc cctgtataaa taattacttc
     8221 gttttgagaa tattctattc tgtcagcttt ctcagataaa aatgttttac agtttccatc
     8281 tgtgatgaga tcaaatattt ttttgctgac tgatttgtaa tctacaattc ctgtctgagg
     8341 tatatgcaat gcacttactc cggaggcgta tggttcgtaa tcttttattt cagattgcga
     8401 aatttctttt atatctttaa gtccgttctc ttcacctctt ctctggagtt ctttcatagc
     8461 ggggatctga tcttcagaag ctgcaattat aatctttccg cagagctcat aaggtatttc
     8521 ataattatta caaaaatcca gtaagagatc ataacctttg atacagttct cagctttcag
     8581 actgccgggt ttataataaa tacccgaatg aataacgccg ctattgtgac cggtctgatg
     8641 agcagatatg tgatcttctt tttcgagaat gcagatcttc agctcctgtg aattttttat
     8701 aagctgcaaa gccgtactga gaccgattat acctgcgccg attattacta catcaaaatt
     8761 tttcatcttt agaatatctc aaaataagta atatgagaat taaatatagt tacaataaat
     8821 ttttaagaaa aaaatctgtt ttcttattaa ataaaaaaac agatttttaa actaaaataa
     8881 attctttgcc acggatcaga aaactattcc ggctataaaa tttaaagtat tattctttaa
     8941 tgtgccgcca ttataaaccg gtgctttgga aatattcaca agaccgagat catctcttat
     9001 ttcaatactg atccccagct ttttacctgc aggaataata attccgattc cgcctgttaa
     9061 cccaaagtct attgattcag tt
//
LOCUS       DAORVQ010000135         9021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4247_length_9021_cov_5.532567, whole genome shotgun sequence.
ACCESSION   DAORVQ010000135 DAORVQ010000000
VERSION     DAORVQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4247_length_9021_cov_5.532567"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLX80_10500"
     CDS             <1..394
                     /locus_tag="PLX80_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21260.1"
                     /translation="MEDLINSFLNNVGIRHDGPMSFRLFLQPAMSLIYAIIAGVKDAK
                     AGNPHFLEGFILGKVDRKTALKEIWKNVGKVFILAAVMEIIFEIIEFKTVHPFEVLKI
                     AFFLAILPYLIFRGIVDRIVSLFIKKGS"
     gene            516..2027
                     /locus_tag="PLX80_10505"
     CDS             516..2027
                     /locus_tag="PLX80_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY21261.1"
                     /translation="MATKQPNILILWGDDIGYWNVSHYSKGMMGYQTPNIDRIGQEGA
                     TFTDWYGQQSCTAGRCAFITGQNPVRPGLTKVGMPGATVGLSAEDPTIAELLKPLGYA
                     TGQFGKNHLGDRNEFLPTVHGFDEFFGNLYHLNAEEEPELPDYPKDPAFKEKYGPRGV
                     MHCFATDTDDATIDPRFGKIGKQTVKDTGPLTMKRMETIDEEITEKALDWMEKQAKAD
                     KPFFLWYNATAMHFRTHLADKNRGKTGQDDYSDRMFTHDEQIGMVLDKLDELGIADNT
                     IVMYSTDNGPENDTWPDGANTPFRSQKDTNWEGAWRVPCVMRWPGKIKPGTVHTQIGS
                     HQDMLPTLLAAAGDPDVSQKLLSGYKIGDMTYKVHIDGFNMIPYLTGEEKESPRKNFF
                     YMSDDGEVMAIRFGDWKCHLAIQRENQMRVWSEPLVKLRVPYMFNLRRDPFERAEYNS
                     NTWLDWTMSHAFIMYQMQDIVAEQIGSFAAYPPRQKPASFNLDAVMEQVSGKK"
     gene            2150..3406
                     /locus_tag="PLX80_10510"
     CDS             2150..3406
                     /locus_tag="PLX80_10510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21262.1"
                     /translation="MNNSDPLNISKVVRNFYEKYPYPGPVESLDKYLDQTEDKKKSRA
                     DFHLYWPHKTFKEDISILIAGCGTSQAAKHAVKFPMAKVTGIDFSENSINSTLKLKKK
                     YSLNNLHVHRLEIENIKELGLTFDKIICTGVLHHLADPDAGLSALRSVLKTGGAMQLM
                     VYAAYGRAGIYMMKEFCRIAGIRADDKGIRDLSDILRSLPPGHPLENLIRDAPDFLHD
                     GSIADSLLNPCDRAYNVPQLFELVNTAGMKFIRWIRQAPYSYHCGVLSRIHGRNDLSM
                     LTPEEQFTAAELFRGNMVRHSIIAYHSDDLAGTEQINFDNENKQWLNFVPIRVPETIC
                     ITERLPADAAAVLINRTHAYRDLILPISAAEKKLFDEIDGIKSIGGIINGNENTGNNS
                     SIMETAAKLFEKLWWFDQVVFDKSRC"
     gene            3602..4282
                     /locus_tag="PLX80_10515"
     CDS             3602..4282
                     /locus_tag="PLX80_10515"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQY21263.1"
                     /translation="MNQIKQPIKFLTDYRGLQFLLFSVFLLLFVIFPVSGNGQFGTIF
                     ANLFFVTVLIFGLLSIDLAAKIKKYLFVLVSAAILTSILGEIYDSQYLILILLVVRLI
                     YLWILVTLIFIKVFKTEDISFKYRITGSITIYLLLGFIWANFYYIFYKLNPGSFKFSI
                     PLNNADNIMFNFIYFSFETLTTLGLGDILPVLPVIKLLVILEGLTGPLYLAILIGRMI
                     SVQSAAKK"
     gene            4415..5419
                     /locus_tag="PLX80_10520"
     CDS             4415..5419
                     /locus_tag="PLX80_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HQY21264.1"
                     /translation="MLKKFYKFKIAVLFVIFLFPFGNANSQISSDPLPSWNDSKVKSN
                     IINFVISVTDPSGPLFVKPLDRIATFDNDGTLWCEQPTIQVEYTFERVKEMFPDHPEW
                     KDEKIFLAVLNGDKEYIEQDIEEGGKGLMKVLAATSTNMTLDEYQNEVLNFFKTAEHP
                     KYKVPFTKVYYVPMIELLNYLRTNEFEIFICSGGGLDFMRVISEEIYGIPTQNVIGSF
                     GKSKFEFSDGITKMERLPELILINDKEGKPVGIDYFIGKKPIFSAGNVRSGGDIAMSI
                     YCQTNMLPSIQLIVNHDDPVREFEYSEKGNESLDAAEKYDWNVISMKNDWKQIFEFEK
                     "
     gene            5460..6869
                     /locus_tag="PLX80_10525"
     CDS             5460..6869
                     /locus_tag="PLX80_10525"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQY21265.1"
                     /translation="MKKVIFVIMLVSLAFSSCGKKEDTESTTERKSSTEADATKTIAK
                     EAFIYGFPMVQLYGIIYSYNIDTKSPDYKGEFNKIHNIPKVFTPADKAIVTPNSDTPY
                     SFLTLDLRAEPIVITVPEIQKDRYYSIMMLDLYTYIYGVAGSRSIGNEAGKFLIAGPD
                     WKGEKPEGIKEVFKCETQFSFVVFRTQLFNPADIKNVIKIQDQYNSEPLSTFLKTTPP
                     PASPVIDWPVFDKKKAEGEGQFQYMNFLMQYCPVDPSEVKLRENFEKLGIVPGKTFNM
                     DSKIQQEIIAGIKAAEDTVKLTTEKDLRNKVSSGDFFGSRKILGDNYLNRFIGAELGI
                     GGLIKEEAEYFPMKTDVAGQPLNTSAGNYEVTFKAGAFPPVNAFWSFTMYDGKTQLLI
                     ENPINRYIINSPMLPELKLNADSSLTIYIQKDSPEKDKASNWLPAPDGDAYVVLRCYW
                     PKEAIINGTWKEPEIAKVN"
     gene            complement(7005..7499)
                     /locus_tag="PLX80_10530"
     CDS             complement(7005..7499)
                     /locus_tag="PLX80_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HQY21266.1"
                     /translation="MSVTDILMIGMWSAFFATSMCILNSTPFKYIIPTFICGFAGVLT
                     RDLLQNTGLSVNWATLAAAFTIVIIAGLIIRSQKVPPIVLICAVLPQWASVSMFQMLN
                     DLRKVTVLTGDDLSKAAVDLTANTAMVLVISLVIAIGFAMGLAVLKLVYREESKKEKT
                     LGME"
     gene            complement(7530..8312)
                     /locus_tag="PLX80_10535"
     CDS             complement(7530..8312)
                     /locus_tag="PLX80_10535"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HQY21267.1"
                     /translation="MPLYEKINSEDLNVSIKSALNAALKVLHFGGSTVMADRTFRNIV
                     SVDKITKFDIMWRLDNLVVSYDSDGQTKTILKSVGSVGTNLILVSKVIEMSEAVRDGK
                     VKVSEVDDELLKISESTPIHSHFIFILVAGLAAAFYSLFHHGSIESVLIVFASAVIGQ
                     TVRLQLQARDIRDSHITFICGLLSAGIASIFLHLGYGQVEIQTLIASLIYLVPGLLMI
                     NGFVDFTKQRYIFIGLQRMLNALFLFLILAFVIITAYTFIKF"
     gene            8589..9020
                     /locus_tag="PLX80_10540"
     CDS             8589..9020
                     /locus_tag="PLX80_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HQY21268.1"
                     /translation="MSDEILKDQQTFDAELKVSDKLAYERTMLAQERTLMATIRTSVS
                     LISFGFTIAKFFQELRKGNMIPEGSTLSSSNLGLWLIIIGTAYIILSTVQHVLILKSL
                     RVPGVDKRISFTFILAVVIALLGLALIISFYSNILSLDLHH"
BASE COUNT         3052 a         1539 c         1662 g         2768 t
ORIGIN      
        1 tatggaagat ttgatcaata gttttttgaa caatgtcggt atccgtcatg acggaccaat
       61 gtcattcagg cttttccttc agccggctat gtctctgata tatgcgatta tagcgggagt
      121 aaaagatgca aaagcaggta accctcattt cctggaagga tttatactgg ggaaagttga
      181 cagaaaaaca gctttaaaag aaatatggaa aaatgtcggt aaagtattta tacttgctgc
      241 agttatggaa ataatattcg agataattga atttaagact gtacatcctt ttgaagtttt
      301 aaagattgct ttctttcttg caattttacc ttatctaata ttcagaggca ttgtggatag
      361 aatagtatca ctttttatta aaaagggaag ttgattgatt ctcagatttt tatatttgaa
      421 tttttaataa attggattta acaaatttgt atttaaaatt taaccggcat tatttaaaac
      481 tatacggaat tcaaaaacca aaaaaataaa taatcatggc tacaaaacaa ccaaacatcc
      541 tgatcttatg gggtgatgac attggatact ggaacgtcag tcattacagc aaaggtatga
      601 tgggatatca gactccaaac attgacagaa ttggacaaga aggtgcaacc tttacggact
      661 ggtacggaca gcaaagctgt actgccggaa gatgtgcttt tatcacaggt cagaatcctg
      721 taagaccggg attgacaaag gtaggaatgc ccggagcgac agttggactt tctgctgaag
      781 atccaacaat agcggaatta ctgaaacccc tgggatatgc tacaggacag ttcggaaaaa
      841 atcatcttgg agacaggaat gaatttctac ctacagttca cggattcgat gagttctttg
      901 gaaatctgta tcaccttaat gctgaagaag aaccggaact tccggattat ccaaaagatc
      961 ctgcattcaa agagaagtac ggaccaagag gtgtcatgca ctgtttcgca actgatacag
     1021 atgatgctac tatagatccg cgctttggaa agatcggcaa acagactgta aaagatacag
     1081 gtcctttgac tatgaaaaga atggaaacca tagatgaaga gatcactgaa aaagctcttg
     1141 actggatgga aaaacaggca aaagcagata agccattttt cttatggtat aatgcaactg
     1201 ctatgcattt cagaactcac ctggctgata aaaacagagg taagacaggt caggatgatt
     1261 acagtgacag aatgttcact catgatgaac agatcggaat ggtcctggac aagctggatg
     1321 aactgggtat tgcagataac actatcgtaa tgtattctac agacaatggc ccggaaaatg
     1381 atacatggcc tgatggcgca aatacacctt tccgaagtca gaaagataca aactgggaag
     1441 gtgcatggcg tgtaccgtgc gtaatgagat ggccgggtaa gatcaagccg ggaacagtgc
     1501 acacacagat cggcagtcat caggatatgc tgcctacttt acttgcagca gccggagatc
     1561 ctgatgtaag tcagaaactt ctttccggat ataagatcgg agacatgact tataaagttc
     1621 atatcgacgg tttcaatatg atcccttatc ttacaggtga agaaaaagaa agtccgagaa
     1681 aaaacttttt ctatatgagt gatgacggag aagtcatggc gatcaggttt ggcgactgga
     1741 aatgtcatct ggcaattcag cgcgaaaatc agatgagagt ctggagcgaa ccactggtaa
     1801 agctgcgtgt accttatatg ttcaatctgc gccgagaccc gtttgaaaga gccgaataca
     1861 attcaaatac ctggcttgac tggacaatga gtcacgcatt cattatgtat cagatgcagg
     1921 atattgttgc ggaacaaata ggaagctttg cagcctatcc tccacgtcag aaacctgctt
     1981 catttaatct ggatgcagtg atggaacaag tgtcaggtaa aaagtaataa ttgtaaattt
     2041 cacaaataag aaaaaattct ccggcagaaa tttttatctg ccggagattt tattttatat
     2101 taaataattt aatactcctt tttatattgt ctgaatgaaa caaactaaaa tgaacaattc
     2161 agatcctctt aacatttcaa aagttgtccg aaacttttac gaaaagtatc cttacccggg
     2221 acctgttgaa agtcttgata aatacctgga tcaaacagaa gataaaaaga agagccgggc
     2281 tgattttcat ctttactggc cgcacaaaac ttttaaagaa gacatctcaa ttctaattgc
     2341 aggctgcgga acatctcagg cggcaaagca tgcggttaag tttccaatgg cgaaagttac
     2401 aggaattgat ttcagtgaga acagtataaa ctctacattg aaactgaaga agaaatatag
     2461 tttaaataat ctgcatgtcc acaggttaga gattgaaaat ataaaagaac tcggtctcac
     2521 atttgacaaa ataatctgta caggcgtact tcatcatctc gccgatccgg atgccggact
     2581 cagtgctttg cgaagtgtgc tcaaaaccgg cggcgcaatg cagttaatgg tttatgcagc
     2641 ttatggaaga gccgggattt acatgatgaa agagttttgc agaattgcag gcatccgtgc
     2701 agatgataaa gggattcgtg atctgagtga tattctcaga tctttgcctc ccggtcatcc
     2761 gctggaaaat cttatccgcg atgcgcctga ttttctacat gacggttcga tagcagattc
     2821 acttctcaat ccatgcgacc gggcttataa tgttcctcag ctttttgaac ttgtcaatac
     2881 tgctggaatg aaattcatca gatggatacg gcaggcgccg tatagttatc actgcggggt
     2941 attgtcccgg attcacggga gaaatgatct cagcatgctc acacctgaag aacagttcac
     3001 agctgctgaa cttttcagag gtaacatggt ccggcacagc atcattgctt accattcaga
     3061 tgatctcgcc ggaactgagc agatcaattt tgataatgaa aataaacagt ggttaaactt
     3121 tgtacctatc cgcgtacccg aaacaatatg catcacggaa agattgcctg ctgatgcagc
     3181 agcagtactc attaacagaa cccatgctta cagggacctg atacttccga ttagtgctgc
     3241 tgaaaagaaa ttatttgacg aaatagacgg catcaaaagt attggcggga tcataaatgg
     3301 aaatgagaat actggaaaca acagttcaat aatggagacc gcagctaagt tatttgaaaa
     3361 gctctggtgg tttgatcagg ttgtatttga taaatcacgg tgttaaaaat ttcaaaactt
     3421 ggaactcaaa ctttacagta aaatctcttt taatacagag ttgatttttc agtctaacag
     3481 tatatttcct tttttaaaat acaggaaatg ttaatataaa caggattaga gttttatttg
     3541 tatatgtgaa gaaaagatat taaatattaa aacaaaaatg aaaaattaat aaaaataata
     3601 aatgaatcaa ataaaacaac ctatcaaatt ccttactgat tacagaggtt tgcagttttt
     3661 acttttctca gtttttcttc tattatttgt gatctttccg gtctcaggta atggtcaatt
     3721 cggtacaata tttgccaatt tattttttgt tactgtttta attttcggat tactatcaat
     3781 agatttagca gcaaagatca aaaagtatct ttttgttttg gtttcagctg caattcttac
     3841 aagtatttta ggtgaaattt atgacagcca atacttaatt cttattcttc tggttgtcag
     3901 attaatttat ctttggatac ttgttacact gatattcata aaagttttta aaactgaaga
     3961 catttccttt aaatacagga tcaccggttc tattaccatt tatcttctgt taggatttat
     4021 ctgggcaaac ttttattaca ttttctataa actgaatccg ggttctttta aattcagcat
     4081 tcctctaaac aatgctgata acattatgtt taattttatt tacttcagtt ttgaaacctt
     4141 aactacactt ggattaggag acatacttcc tgttttacct gtaataaaat tacttgttat
     4201 actggaagga cttaccggtc ctttatatct agcaatactc attggacgga tgatatcagt
     4261 gcagtcagca gctaagaaat aagtgataat taattcaata gaacaggtct ctgtttatat
     4321 attactgata ttttagagac taataaattt aatctgattt tattaaattg gcattaatct
     4381 ttccaaatct tatataaatc taaatcaaag tattatgtta aaaaaatttt acaaatttaa
     4441 aattgcagtt ttatttgtta tttttttatt tccgtttgga aatgcaaaca gtcagatcag
     4501 ctctgatccg cttccttcct ggaacgattc gaaagttaaa tccaacatta taaattttgt
     4561 aatttctgta acagacccgt caggtcctct ctttgtaaaa cctcttgatc gtatcgctac
     4621 atttgataat gacgggacgc tttggtgcga gcagcctaca atccaggtgg aatatacatt
     4681 tgaaagagta aaggaaatgt tccctgatca tcctgaatgg aaagatgaaa agatattttt
     4741 ggctgtatta aatggagaca aggaatatat tgagcaggat atcgaagaag gaggaaaagg
     4801 tctgatgaaa gtacttgctg ctacgagcac gaatatgaca ttagatgaat accagaatga
     4861 agttctgaat ttttttaaaa cagcagagca tccaaaatac aaagtaccgt ttacaaaagt
     4921 ttattatgta cctatgattg aacttttaaa ttatcttaga actaatgaat ttgaaatttt
     4981 tatctgctca ggcggcggac ttgattttat gagagtcata tctgaagaga tctacggaat
     5041 ccctactcag aatgtgatcg gcagttttgg taaatcgaaa tttgaatttt ctgacggaat
     5101 tacaaaaatg gagaggttac ctgaattaat acttatcaat gataaagaag gtaaacccgt
     5161 cggcatagat tatttcattg gtaaaaaacc aattttttct gcagggaatg tccgatcagg
     5221 aggtgatatt gcaatgagta tttattgtca aactaatatg ctcccttcca ttcagcttat
     5281 agtaaatcac gacgatcctg tcagagaatt tgaatacagt gaaaagggaa atgaatcctt
     5341 agacgctgct gagaaatatg actggaatgt tataagtatg aaaaatgact ggaagcagat
     5401 ttttgagttt gagaaataat tcagaattaa agattttttt agaaaattaa aactatataa
     5461 tgaaaaaagt aatatttgta ataatgttag tatctcttgc attcagttcc tgcggtaaaa
     5521 aagaagatac tgaatctaca actgaaagaa agtcctcaac agaagcagat gcaacaaaaa
     5581 ccattgcaaa ggaagctttt atttacggtt ttccaatggt gcaattatat ggaataattt
     5641 attcttacaa tatagacaca aaaagtcctg attacaaagg tgagtttaat aagatacata
     5701 atataccgaa agtatttaca cctgccgata aagcaattgt aactccaaat tcagatacac
     5761 catattcatt tttaacattg gatctgagag ccgaaccgat tgtgattact gttcctgaaa
     5821 ttcaaaagga ccgatactat tctattatga tgcttgattt gtacacatac atttatggag
     5881 tagccggcag cagatcgata ggaaatgaag caggtaaatt tttaattgct ggtccggact
     5941 ggaaagggga aaaacctgaa ggcataaagg aagtcttcaa atgtgaaact cagttctctt
     6001 ttgtagtttt caggacacag cttttcaatc cagctgatat taaaaatgtc ataaagattc
     6061 aggatcagta caattctgag ccgttaagta catttttaaa aactacacct ccccctgctt
     6121 cacctgtaat tgactggccg gtatttgata agaagaaagc tgaaggtgaa ggccaatttc
     6181 agtatatgaa ttttttaatg caatattgtc cggttgatcc ttctgaagtg aaactcaggg
     6241 aaaattttga aaagctaggt atcgttcccg gcaagacttt taatatggat tcaaaaatac
     6301 aacaggaaat tattgcaggg atcaaagctg cagaagatac tgtgaagctg acaactgaaa
     6361 aagatttaag aaataaagtt tcttccggtg atttctttgg atcaaggaaa attctgggtg
     6421 ataattattt gaacagattt attggtgctg aattagggat tggaggattg ataaaagagg
     6481 aagcagaata tttcccgatg aaaaccgatg tcgctggtca gccgctgaat acctctgccg
     6541 gcaattatga agtaacattt aaagccggtg ctttcccgcc tgtaaatgcc ttttggtctt
     6601 ttacaatgta tgacggaaag acacagctac tgatcgaaaa tccaattaac agatatatta
     6661 taaattcacc gatgcttcct gaattaaagc tgaatgctga cagttcactt acaatttata
     6721 ttcaaaaaga ttcacctgaa aaagataaag catccaactg gcttccagcg ccagacggag
     6781 acgcttatgt agtattaaga tgttactggc caaaagaagc aataatcaat ggtacatgga
     6841 aagaaccgga aattgcgaaa gtaaattaat aaatttccgg tctgttttaa tttgataacg
     6901 tgcagtattt ttaataatgc tgcacgtttt ttatgataaa atgtaatgta aatataatgg
     6961 caacgattat ttttaatata taaatttcta aagtttttat tgaattactc cattcccagc
     7021 gttttctctt tttttgattc ttcacggtaa acaagtttca atactgcaag tcccatcgca
     7081 aaaccaattg caattacaag agatatgact aaaaccatag ccgtgtttgc agtcagatct
     7141 actgcagctt tactaagatc atcgccggtt aagacagtaa cttttctgag atcattcagc
     7201 atctgaaaca ttgagaccga tgcccattga ggcagcactg cacagatgag tactatcggc
     7261 ggaactttct ggcttctgat aattaatccg gcaattatta ctatagtgaa tgccgctgca
     7321 agtgtagccc agttaacact cagcccggta ttctgcaaaa gatccctggt aagcacacct
     7381 gcaaatccgc atataaaagt cgggataata tatttaaacg gtgtggaatt caatatgcac
     7441 atacttgtag caaaaaatgc cgaccacatt ccaatcatta aaatatctgt aacactcata
     7501 atatttttcc tttttaaggc taaagaaatt taaaatttta taaaagtata agctgtgatt
     7561 ataacaaaag ccaaaattaa aaataggaat aatgcattca gcattctctg cagtccgata
     7621 aaaatatatc tctgtttagt aaaatcgaca aatccgttta tcataagtaa tccgggtaca
     7681 agataaataa ggctggctat taatgtctgg atctcaacct gtccgtaacc caaatgaaga
     7741 aatatacttg caattcctgc agataataat ccgcatataa atgtaatatg actgtcacga
     7801 atatccctcg cctgcaactg cagtctaact gtctgaccaa taactgcaga agcaaacact
     7861 atcaatacag actctatcga tccatgatga aataaagaat aaaatgcggc agcaagtcct
     7921 gcaacaagta taaaaatgaa atgtgaatga ataggagtcg attccgaaat tttcaacaat
     7981 tcatcatcca cttctgaaac ttttactttt ccgtctctga ctgcttcaga catttctatt
     8041 acttttgaaa ctaagatcag attagttccg accgatccta ccgattttaa aatcgttttt
     8101 gtctgaccat ctgaatcata acttacaact aaattatcta accgccacat gatatcaaat
     8161 tttgtaattt tatctacact tacaatattt ctgaaagtcc tgtcagccat tacagtcgat
     8221 cctccgaaat gcagaacttt taatgcagca ttcagtgctg actttattga tacatttaaa
     8281 tcttcagaat ttattttttc gtaaagcggc atggattgat ttgaaaatta attaaagggt
     8341 ttcaggatca attataatta ttcagtctgt aaaaaatctt tcacaattta atttttaaat
     8401 attaaaatta aaacttaaaa aaaatttcga aagtcttatc cacacttaat attaaaatta
     8461 tgctttaata taaattaatg ttgatcttta aaattctatt aaatttaaat taacatttac
     8521 agttttaatc actattttaa ttttttacaa ttccccaata aaatataaaa cttaatttaa
     8581 aataaatctt gagtgacgaa attttaaaag accagcagac ttttgatgct gagttaaagg
     8641 taagtgacaa acttgcatat gaaagaacta tgcttgcaca ggaaagaact ctcatggcaa
     8701 caattaggac atcagtttcc cttatcagtt ttggttttac aattgcaaaa ttttttcagg
     8761 aattaagaaa aggaaatatg atccctgagg gatctacttt gtcttcatcc aatcttggac
     8821 tctggctgat aattatagga accgcttaca taattctttc gactgttcag catgttttaa
     8881 tactgaaatc attacgcgtt ccgggagttg ataaaagaat ttcttttact tttattttag
     8941 ctgtagtaat tgctttactt ggtctggcat tgatcatatc attttactca aatattcttt
     9001 cactggatct gcatcattaa a
//
LOCUS       DAORVQ010000136         9015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4249_length_9015_cov_5.631362, whole genome shotgun sequence.
ACCESSION   DAORVQ010000136 DAORVQ010000000
VERSION     DAORVQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_9015_cov_5.631362"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /gene="truA"
                     /locus_tag="PLX80_10545"
     CDS             complement(<1..699)
                     /gene="truA"
                     /locus_tag="PLX80_10545"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQY21269.1"
                     /translation="MKFKYILEYDGTNYSGWQVQKNAKTVQGVLIRCAKAMLDETKGK
                     SEFIDFQGSGRTDKGVHAKAQVGHLECETMLAPHIQKMKMNDALPSDINVLKIDRVPN
                     NFHARHSAISRQYVYVISKRRSAFEKKYVWWVKDELDVDKMKKLSELLVGFKDFRSFS
                     EPDPNNKSTTVNLESLTIEDTDDKIFIRIKASHFLWKMVRRIVGTLTEGGRGNLGTKE
                     IKKYLNTYSKETAAF"
     gene            complement(1142..1936)
                     /locus_tag="PLX80_10550"
     CDS             complement(1142..1936)
                     /locus_tag="PLX80_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY21270.1"
                     /translation="MNTFKVNGISVTDYEGTGEPLIFIHAYPLCNRMWDEQVNFFKDK
                     YRVITYDIRGLGYSNEIDSYLFTMEDLADDLEDILDHLKLDKVHACGLSVGGYILLRA
                     ADRMPERFLSLILADTKADSDDYDGLLGRSAAIKKIRSGGKEEFINAMLPKLISEKNI
                     ENTELTGKLKEMMGWMDADGMCAVMIAIATRTNLSYKIKEFEIPSLVIVGKDDVLTPV
                     IKSFYLKEGMKNSTYHLIFDAGHISNMEKPEEFNLAVNNFLDSSGS"
     gene            complement(2042..2776)
                     /locus_tag="PLX80_10555"
     CDS             complement(2042..2776)
                     /locus_tag="PLX80_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HQY21271.1"
                     /translation="MAKIVLDNIVNEPFFGFDKKALQFLKTLKVSKNNNKDWFDKNRD
                     TYENFIKAPMKDLIDVLAVEIKKIDPNIVVNYKSIFRINRDIRFSKNKIPYKTHYSAA
                     FAFGKVKSSEIPQFYFHFSPDEYVFAAGQYSMDTVMLKKIRAYIFNNFSEYKKITGNK
                     TFTKTFGEVKGETLTNLPKEFDKEIINSIDKKDKLLENILKMKQFYVFSNDNPDVILD
                     EKIVDKIINDIKITYDFTKFLHKATI"
     gene            2924..7939
                     /locus_tag="PLX80_10560"
     CDS             2924..7939
                     /locus_tag="PLX80_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HQY21272.1"
                     /translation="MKTPISMHLIIGLIFSFSVLSVQSYAQRDNSNFNISTKNIYSPG
                     DKIDMALNSYGNSEDNDKKTITYEIEVLKITDIEQFYSAQTSRYNTDVLGQDNKNLLY
                     FTKSIYSFKKKLLSNYENGYSYLNEVVPININDKGAYLIKVTSEKKVAYCGFVISELG
                     LVSKAGNNSMLGYAVQRRSGTPVNDAELNFYIGTKKIGQGKTTDGIFFQTVNPDVKTD
                     ADENSQPIIIGSYGDDILISDPYLYFGYAGNKYFTYIYTEQPVYRTGLDVNFKGIIRQ
                     NEFNTLKPLGNKELTVIIKDSKNAEVYKEVLNTNEMGSFNGTLKLAEEAALGTYTIYA
                     NIDENNSYSSSFEVEQYKKPEFKVTVTPDKSQYFNKDNLTAEVDAKYYFGSNVADAEI
                     EYNIYKVRYYRPWWTFSEYSWWYEDYYENADDNQKFSGAEFLYTGNGKLDSEGRFQIN
                     YFIEEEFKSENNYYDWYRPYNYDSDYRYIIQAKVTDKSRREISGTSTVFVTRGGFTLS
                     AKTDKYMYKPNDNVKLTVFAEDFSSNPVKTNFEATIYRTTWTKDGKDTKDYIKSVSGQ
                     TLSDGQGSVIFDIENLNSEGYYNIEIKSKDDRSNEITANTGFYVSKGDMWWYYNQSGT
                     VQIIPEKDSYRQGEICKALVLINHPDANVLITSNTDDILYFKTEKFTGTSKIIEIPVT
                     DKYFSNFDINISYVKDGMFYNSSKPIMVIPEEKFLTVTIDPSKLIYKPKESAEVKVKV
                     IDSYGNPVRNAEVSIAVVDESIFAIKEDRTKDIRKFFYGNRYTSVSTSFNNELNSSGS
                     SRLISIYEKFNLRNTDDKDLATVKGRLMNKKNEPISNAAIIIDEDYYAAVTGVDGSFE
                     FKLPEGKYSIGVSYSKKEENELKEIILVKGQVKTITLYNNREMNEEEYIDDSGSRGVD
                     NIASTTSGVVIDEINVRGGRNEVSPDQSGKIMKKGFDTGELVSADVRSDFRDAIFWSP
                     YSVTDAEGYATVNFNFPDNLTEWRITSRVITEDTKVGQMVSTIITRKDLLVRMETPRF
                     LQENDEVTISTIIHNYLKTEKQTKVSFRADNVRLLNDEKVKTVSIPPNSEIRLDWNIK
                     INEPAGESVLYAEALTDEESDAVEVKIPLQPEGLEVIKNEIADFSDEFKTELKTVEIP
                     SGTDLRSSGMKFTVDASLASVILSSLDELAGYPYGCVEQTMSRFLPTVIVAKAFKEID
                     APISEATKKDLPLMVAKGLERLYGFQQSDGGWGWWHNDNSNPFMTAYVVYGLAIAAET
                     GYEIRDGVLNNGKSALKNHISGNEKDMTTKAYMLYALAVSGKENNDFIKDNLEDIKSS
                     ELNDYGKGLVAMTWKLIGNESKAKDELVSLEKDVKFTGESAAYWEGKEFHYRWQDDKV
                     QTTAMALKAIVNINDQSDLKEKVVRWLMLQRRGNAWRSTQETAMIIYAMVDYLKTSNE
                     LDPNYNVKVFVNNNSVYEKSMSRENVFAKSETIQIDNKFLKTGQNEIRIEKSGRGKVY
                     FSSGTSYYDNTAGNKSSEKGFRVEKEYFKLEKYEAYSENKITYRKKYFDGSLKSGDEF
                     LVKIRVSSKDDDLNYFMLEDFIPAGAEVIKDDWAYKVEDENDYQGYDHYRWRWWFADK
                     EIRDNRVVFFASYMGKGEYQFSYIMRAQIPGEYSVNPARGMLMYYPEVNGNSNEMQVT
                     ITD"
BASE COUNT         3141 a         1408 c         1594 g         2872 t
ORIGIN      
        1 aaaagctgct gtctcttttg aataagtgtt aagatatttt ttaatttcct ttgttcctaa
       61 atttcctctg cctccttcgg taagtgtccc gacaattctt cgtaccatct tccataaaaa
      121 atgagatgcc tttatacgga taaatatttt atcatcagta tcttcaatcg tcagagattc
      181 taaatttacc gttgtggatt tattatttgg atcaggctct gaaaatgatc tgaagtcttt
      241 gaatccaact aataattcag ataacttttt catcttatca acatccagtt catcctttac
      301 ccaccataca tatttttttt caaatgcaga tcttcttttg gaaatcacat atacatattg
      361 tctggatatt gctgaatgcc gggcatgaaa attattcggt actctgtcaa ttttcaaaac
      421 atttatatca gaaggaagag catcattcat tttcattttt tgaatatgcg gcgcaagcat
      481 tgtctcacac tcgagatgtc cgacctgtgc ttttgcatga actcccttat ctgtccttcc
      541 ggatccctgg aaatctataa actcactctt tccttttgtc tcgtcaagca tcgcctttgc
      601 acatcttatc agaactcctt gtacagtttt tgcatttttc tgtacctgcc agcccgaata
      661 attagttcca tcatactcta aaatgtattt aaatttcatt actgtttatt tttcaaaaaa
      721 attaatcgca tgaaaacggt ttcaaatgat agcattgtta ctaaataaaa tggttctgaa
      781 tgatttatat tcgaaagttg tattaaaaaa tattaacatt gcaaaaaagt tgaacagcga
      841 agatataatt caaatagcaa tacaacaccc acaaaaacta gtctttcccg cgaatgatgg
      901 aatatcaaac taaaatcaga catttaatct aaacttaacc aaaaaaatcc ttttttctaa
      961 ttcgatatta aataaatacc aaacatcctt atagcttcta cttaattcag attcccgcct
     1021 tagagggaat gacaattttt gaagcattcg ggaaagaatg aatctgttaa aaaatatttt
     1081 ctaaattatt ttcaatgtaa gtcataatgc accaaaaata ataataagta tctattaaaa
     1141 tttaactacc tgatgaatca agaaaattat ttacagcaag attgaattct tccggctttt
     1201 ccatattact tatatgacct gcgtcaaaga taagatggta agtcgaattt ttcattccct
     1261 ctttcaaata aaatgatttt atcaccggcg ttagtacatc gtcttttcct actataacaa
     1321 gtgacggaat ttcgaactct tttattttgt aagataaatt tgttcttgtt gcaatagcaa
     1381 tcattacagc acacattccg tcagcatcca tccatcccat catttctttt aacttccctg
     1441 taagttcagt gttctcaata ttcttctcac tgatgagctt tggcagcatt gcatttataa
     1501 actcttcttt ccctcctgac ctgatctttt taattgctgc cgatcttccc agaagtccgt
     1561 cgtaatcatc tgaatcagct tttgtatctg caagaatcaa agataaaaat ctttcaggca
     1621 ttctgtctgc tgctctcagc aaaatatatc cgccgacaga taatccgcat gcatgaactt
     1681 tatccagttt caaatgatcc agaatatcct caagatcatc tgcaagatct tccattgtaa
     1741 ataaatatga atcaatttca ttactgtaac caaggcctct gatgtcataa gtgatcactc
     1801 tgtatttatc tttgaaaaaa ttaacctgct catcccacat tctgttacac agcggataag
     1861 catgaataaa gatcagcggc tctcctgtac cttcgtagtc tgtgacacta attccgttta
     1921 ctttaaaagt attcataaaa aattattaaa gaataattaa tattaaaaat ttaattcagt
     1981 atcttataaa ccgcagagtc cgaagagcac gtgactctgc cggaaaatat ttaaattgaa
     2041 attaaatagt tgctttgtga agaaatttcg taaagtcata tgtgatcttg atatcgttaa
     2101 taattttatc aactattttt tcatccaata tcacatcagg attgtcattg ctgaaaacat
     2161 aaaactgttt catcttcaaa atattctcca atagtttatc ttttttatca atagaattta
     2221 tgatctcttt atcaaattct ttaggtagat ttgtaagagt ctctcctttc acttctccaa
     2281 aagtttttgt aaatgtttta ttgcctgtga tttttttata ttcactgaaa ttattaaaga
     2341 tataagctct tatttttttc agcattacag tatccatgct gtactgacct gcagcaaata
     2401 catattcatc aggactaaaa tgaaaataaa actgtggaat ttcagaagat tttactttgc
     2461 caaaagcaaa tgctgcagag taatgagttt tgtatggaat cttatttttt gaaaatctta
     2521 tatcacggtt tattctgaaa atggatttat aatttacgac aatattcgga tcgatctttt
     2581 ttatttctac agccagtacg tcgatgagat ctttcatcgg agcttttatg aaattttcat
     2641 atgtatccct gttcttatca aaccaatcct tattgttgtt tttgctgact ttaagggttt
     2701 tgagaaactg aagagctttt ttatcaaatc caaaaaaggg ttcgtttaca atattgtcta
     2761 aaactatttt tgccatataa atttttataa aaaaaatttc agggaacttt caaaggatta
     2821 tttaatataa gacctgtaac ttttctatag catcaatcta attattaaat attactttta
     2881 aaatttaaaa atatttattt cccctaatta aaactttaca gtcatgaaaa ctcccatttc
     2941 tatgcattta atcattggtt taattttttc tttttcagta ttatccgtac aaagttatgc
     3001 tcaaagagat aattctaatt ttaacatcag cacaaaaaac atctattcgc cgggtgataa
     3061 aattgatatg gcattgaatt cctatggaaa ttctgaggat aatgataaaa agactatcac
     3121 atatgaaatt gaagttttaa aaattacaga tatagagcag ttctattctg cgcaaacttc
     3181 cagatataat actgatgtac ttggtcagga taataaaaat ttactttatt tcacaaaaag
     3241 tatttattca ttcaaaaaga aattattatc caattatgaa aacgggtaca gctatcttaa
     3301 tgaagtagtc cctataaata taaatgataa aggtgcatat ctgataaaag taacttctga
     3361 aaaaaaagta gcttactgcg gatttgtaat ttctgagctt ggattggttt caaaggctgg
     3421 taataattct atgctgggat atgctgttca gagaagaagt ggtacgcctg taaatgatgc
     3481 tgaactgaat ttttatattg gtacaaaaaa gatcgggcaa gggaaaacta ctgacggtat
     3541 attttttcag actgtgaatc ctgatgtaaa aacagatgct gatgagaata gtcagccgat
     3601 aattattgga agttacggag atgatattct gatttcagat ccatatttgt attttggata
     3661 tgcagggaat aaatatttca cttacatcta tacagagcaa cctgtataca gaacgggtct
     3721 tgatgtgaat ttcaaaggaa tcatcagaca aaatgaattc aatactttaa agcctttagg
     3781 aaataaagag cttacagtta ttatcaaaga ttcaaaaaat gctgaagtgt ataaagaagt
     3841 tctgaacacg aatgaaatgg gaagttttaa cggaactttg aaattagctg aagaagcagc
     3901 cctcggtaca tatactattt atgcaaacat tgatgaaaat aattcttact catcgagctt
     3961 tgaagtggag cagtataaga aacctgaatt taaagttact gtcactcctg acaaaagcca
     4021 gtatttcaat aaagataatc tgactgcgga agtcgatgcg aaatattatt tcggaagtaa
     4081 tgttgcagat gcagagatcg aatacaatat ttataaagta agatattaca gaccatggtg
     4141 gacattctcg gagtattcat ggtggtatga agattattat gaaaatgcgg atgacaatca
     4201 aaaatttagc ggtgctgaat ttttatacac aggtaatgga aagctcgata gtgaaggcag
     4261 gtttcagata aattatttta ttgaggaaga attcaaaagt gagaataatt attacgactg
     4321 gtacagacct tataattatg attcggatta cagatatata attcaggcaa aggtaactga
     4381 taaatcgcga agggaaatat caggaacatc tactgtcttt gtgacaagag gaggatttac
     4441 attatcagca aagacggata aatatatgta caagccaaat gataatgtaa agctgaccgt
     4501 gtttgcagaa gacttttcga gcaatcctgt aaagacaaat ttcgaagcaa cgatctacag
     4561 aactacctgg acaaaggacg ggaaagatac aaaagattat attaaatcgg tttcgggtca
     4621 gacactttcg gacggacagg ggtcggtaat ttttgatatt gagaatttaa attctgaagg
     4681 atattacaat atcgaaatta aatccaaaga cgacagatca aatgagatca ctgcgaatac
     4741 cggattttat gtgagcaaag gcgacatgtg gtggtattat aatcagtcgg gcactgttca
     4801 gataattccc gaaaaagaca gctacagaca gggtgaaata tgtaaagctc ttgtactgat
     4861 caatcatcct gatgcaaatg tactgatcac ttcaaatacg gacgacatac tttatttcaa
     4921 aacagaaaaa tttacaggca cttctaaaat aattgagatc cctgtgacag ataaatattt
     4981 ctcgaatttt gatattaata taagttatgt aaaagacgga atgttttata attcaagtaa
     5041 accgataatg gtaataccgg aagaaaaatt tctgaccgtt accattgatc cttcaaagct
     5101 tatttataaa ccaaaagaat cagcagaagt caaagtaaaa gtaatcgaca gttatggcaa
     5161 tcctgtgagg aatgctgaag tctcgatcgc agtagtggat gaaagtattt ttgcaattaa
     5221 ggaagacagg actaaagaca taagaaaatt cttttacggc aaccgatata catcagtctc
     5281 tacttcattt aacaatgagc tgaacagctc cggcagttcg agacttattt ccatttatga
     5341 aaaatttaat ctgcgaaaca cagatgataa agatcttgcc acagtcaaag gaagattaat
     5401 gaataaaaaa aatgaaccaa tcagtaacgc agcaataatt attgatgagg attattatgc
     5461 agcagtgaca ggtgttgacg gcagctttga atttaaacta cctgaaggaa aatattctat
     5521 tggtgtcagc tatagtaaaa aagaagaaaa tgaactcaag gaaattatac tggtcaaagg
     5581 tcaggtgaag acaattacat tatataataa cagggaaatg aatgaagaag agtatattga
     5641 tgattcagga tcaagaggtg ttgataatat tgcttctact actagtggag tagtgataga
     5701 tgagattaat gtcaggggag gaagaaatga agtttctccg gatcaaagcg gtaaaataat
     5761 gaaaaaagga tttgatacag gagagttagt ttctgccgat gtcagatcag attttcgtga
     5821 tgcaatattc tggagtcctt attcagtgac tgatgcagaa ggttatgcta ctgttaattt
     5881 taattttcct gataatctga cagagtggag gatcacttca cgcgtgatca ctgaagatac
     5941 aaaagtaggg cagatggttt ctacaatcat tacaagaaaa gatcttttgg tcagaatgga
     6001 gactccaaga tttttacagg aaaatgacga agttactatt tcaactatta ttcataatta
     6061 tctgaaaaca gaaaagcaga ctaaggtttc attcagagct gataatgtaa gactcttgaa
     6121 tgatgaaaaa gttaagactg tcagcattcc acctaattct gaaattcgtc tggactggaa
     6181 tataaaaata aatgaacctg caggcgagtc agtactttat gctgaagcac ttacagatga
     6241 agagtccgat gcagtagagg taaagattcc gctgcagcca gaaggtcttg aagtaataaa
     6301 aaatgagatc gcagattttt cggatgaatt taaaactgaa ttgaagactg tagaaattcc
     6361 gtccggaact gatctgagaa gttcgggaat gaagtttact gttgatgcat cacttgcttc
     6421 agtgattctt tcttcacttg atgaactagc cggttatcca tacggatgtg tagagcagac
     6481 gatgagcaga ttccttccga cagtaattgt cgcaaaagca tttaaagaaa tcgatgcacc
     6541 gataagtgaa gccactaaaa aggatctgcc tttgatggtt gcaaaaggtc ttgaaagact
     6601 atacggtttt cagcagtctg acggaggctg gggatggtgg cataatgata attcaaatcc
     6661 gttcatgaca gcttatgttg tatatggatt agcgatcgca gcagagaccg gttatgagat
     6721 aagggatggt gttctcaata atggtaaatc tgctttgaaa aatcacatca gcggcaatga
     6781 aaaagatatg actacgaaag cttatatgct gtatgctctt gcagtttcag gaaaagaaaa
     6841 taatgatttt ataaaagata accttgaaga tataaaatct tctgaactta atgactatgg
     6901 aaaaggtctg gttgctatga cctggaagct tatcggaaat gaaagtaagg caaaagatga
     6961 actggtgagt cttgaaaagg atgtaaagtt cacaggtgaa agtgctgcct actgggaagg
     7021 aaaagaattt cattacagat ggcaggatga taaagttcag accactgcaa tggcgctgaa
     7081 agcgatcgtg aatattaatg accagtcaga tctgaaggag aaggttgtaa gatggcttat
     7141 gctgcagaga cgcggcaatg catggagaag tacgcaggag acagctatga taatttatgc
     7201 aatggtagat tatttaaaaa catcaaatga acttgaccca aattataatg tgaaagtatt
     7261 tgtaaataat aattctgttt atgaaaaatc tatgagcaga gaaaatgtat ttgcaaaaag
     7321 tgaaaccata cagatcgaca ataaattttt gaagacaggt cagaatgaaa taaggattga
     7381 aaagtcaggc agaggtaagg tttatttctc atctggcact tcatattatg acaatacagc
     7441 aggaaataaa tccagtgaaa aaggattcag agtggagaag gaatatttta aacttgagaa
     7501 atacgaagca tattctgaaa ataaaattac ttacagaaag aaatattttg acggcagtct
     7561 gaagtcggga gatgaattcc ttgtaaagat cagagtttct tcaaaggatg atgatctgaa
     7621 ttatttcatg cttgaggatt ttatacctgc cggtgctgaa gtaataaaag acgactgggc
     7681 atacaaggtc gaagatgaaa atgattatca aggttatgat cattaccgct ggagatggtg
     7741 gtttgcggat aaagagatca gggataaccg ggtagttttc tttgcgagtt atatgggaaa
     7801 aggggagtat cagttctcat atataatgcg agcacagatc ccgggcgaat attcagtaaa
     7861 ccctgcccgt ggaatgctga tgtattatcc tgaggtgaat ggaaattcga atgagatgca
     7921 ggtaacgatt acagattaag tttatttttt aaaaattatc atccgcagaa tttttaaacc
     7981 tgcctcaaag gctcaaagct aaatgagttt attcttaatg ttttttagtt ttgagtcttg
     8041 cagccttaga ggcaatccga aaaataaaat tttgttattc cagcgtgctc ttagctggaa
     8101 tccggggatt ggtttacaag tatatattca aagtttaaaa gtaatacttc cagagtgcag
     8161 ctgtgtcgat tttaatattt aattcttaca tcagctgatt atattactct gcgtgaactc
     8221 caattataaa tctggttttt ttgaaccgct gatgacgctg attagaatga ttaacatgat
     8281 ttttaaacag atgaatttga aagttttcga atctatgaaa tcagtttaat ctgaaattat
     8341 aataataaat atcaaatccc gtggggcgat atttttgtag aaatgcgggt aaaataaata
     8401 tcaaatccca taggggttgc attattattc gtaatgatca gcatttgttg atttgaataa
     8461 attgttttaa ataaattcac cccttcgggg ttaacaggat aaaagtttat atattaatat
     8521 ttataataaa aatccgaatc agcgtaatca ttttaatcag cgtaatcaac tttcagatat
     8581 ttacaaaatc tcataaattt caaatattcc acaaaattct tacaattttc gacagctata
     8641 ttttttaccc tgttttaaca aaccctttgt cagcctgact aaaatcgatg ccaaaaacca
     8701 ttttttcaca aactgccttt tttaccattt gactcgtttg cttattatta ctaatttgtg
     8761 aatggtaaaa caagaggtca ttaaaggaag aaagaattca atatctgctg ctgttttcta
     8821 aattgaaaca gcagcagaga attgagaatt aattcagtga aatttatcag gctgaataat
     8881 tagtccaatt aaattcacag aaattttaaa cttttaaaaa aattttagta tttgattaaa
     8941 tacttttata aaaataaaac atgaataaac tctttacaac atttctaata atcatctcag
     9001 ctatctcaat aaaca
//
LOCUS       DAORVQ010000137         9003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4257_length_9003_cov_5.252123, whole genome shotgun sequence.
ACCESSION   DAORVQ010000137 DAORVQ010000000
VERSION     DAORVQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_9003_cov_5.252123"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLX80_10565"
     CDS             complement(<1..363)
                     /locus_tag="PLX80_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21273.1"
                     /translation="MNLFLSKEKGLDSYLDKAIWFSIIALISTAIFSIALLQIFTSSA
                     IILWIVKIIVTKNYTFVKTPLDKIFLIFIAARILSIPLSLYPEISYIRFLKEIIFYTI
                     FFVVTNEFDVTNRKKIILV"
     gene            complement(360..1202)
                     /locus_tag="PLX80_10570"
     CDS             complement(360..1202)
                     /locus_tag="PLX80_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21274.1"
                     /translation="MKVSGFTIVRNGELYAYPYVEAIQSILPLCDEMIVNVGKSNDNT
                     LEIIRAIDNKKIKIFESEWDMSLKGGEVLSVETNKALDKCTGDWCFYIQGDEVLHEKF
                     FENVKKAMSVNLNKPNIEGLRFWYKHFYGSYDYVQDNYRKWYIREVRIVRKKDNIISW
                     GDALDFRHPDGSKLNSKDIDAEIYHYGWVRPPEKLLNKRRDFEKLYNEGETAEKNISG
                     FQNYDDLGNLEKFDGSHPEVMKKRVSESKWEFDAKLSEQNPDWIRKILIFLNPLLKRI
                     NKSE"
     gene            complement(1199..3034)
                     /locus_tag="PLX80_10575"
     CDS             complement(1199..3034)
                     /locus_tag="PLX80_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21275.1"
                     /translation="MHNYKRIFKYVKPYLKQLITANIFTILVVIFSLLSVMMIFPFMD
                     LLFNDAPKTVPDKEITSIFDLKDFIIVYFAKILMNYDKLDVLKYLCILIFLTFFLKNL
                     FSYLQTYFMSVVEQGILKDIRRDLYDHYLELPLSFFTDERKGNLISRIINDVQIIKDS
                     LIAVLNSLFRDPPTIIVFSTILFLFNWKLTLIIFLMAPITAIILSKIGNSLKRSSQKS
                     QEKISDITSTLDETLGAVRVVKAFGMEEYEKQKFEKENYSYFELLVKIFRKRALASPV
                     SEIIGVITIVIILFIFGSDIIKGSSDMTPGAFIFYLAVFFQMMPSLKLFGQMFNSYKE
                     GSAASERVFKLLDTKPDIVNKPDAKELKEFSSYIEFKDVSFKYEKSDMILRNVNLTIE
                     KGKIIAIVGPSGAGKSTLVDLIPRFYDAVSGSLQIDGTEIKDLTIESVREKMGIVTQE
                     TILFNDSVKNNIAYGKQDISHEKIIDAAKAANAHIFIEKFENGYDTVIGDRGVKLSGG
                     ERQRLSIARALLKNPPILILDEATSSLDTESELLVQQAIERLMIGRTSIVIAHRLSTV
                     RNADMIVVLSKGEIIETGTHDELLLNESIYKKLYNLQFRHQEIIL"
     gene            complement(3035..3793)
                     /locus_tag="PLX80_10580"
     CDS             complement(3035..3793)
                     /locus_tag="PLX80_10580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21276.1"
                     /translation="MNRISGLIICRDEENNIEDCIKSIQWCDEIVVIDSFSKDRTLEI
                     VKKFTDKVFQNEWEGFSKQRKFALSKVNSEWIFSLDADERCTEELALEIRSILDSSNN
                     VNGYLIPRKSFFLGKWIQHCGWYPDHQMRLFKRDKVTVSERLVHEGYNVTGETTKLKS
                     DILHYTVRSVTEFAEKINHYSALSALEKSDGKKISMSYLFFKPFFEFKKKFFFQQGFR
                     DGIYGLMVSFFHMITKILTYMKMIEIQNKKDNRK"
     gene            complement(3794..4633)
                     /locus_tag="PLX80_10585"
     CDS             complement(3794..4633)
                     /locus_tag="PLX80_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21277.1"
                     /translation="MHKVTLIISVYNKVKELELIFHALSIQSFKDFEVIIAEDGMNEE
                     MRSLINKWKSSDNFPVKHLTQEDIGFRKNRILNGSIRNASSDHLIFFDGDCIPHSDFV
                     KAHFENRAENTVLCGRRVNLTKKISEKITKESILNKEYEKLKLSEVIYSSLNKGKNEF
                     NFNIEEGIIIKNRSVRNIVTNQDEHILGCNFSVPKELLIKINGFNENYEGPGLGEDSD
                     IEFRLRLINAKFISVRNLAVQYHMYHPKTIEEEKNMEYFNEIKKKKEFFCKNGLEKIN
                     LID"
     gene            4918..6222
                     /locus_tag="PLX80_10590"
                     /pseudo
     CDS             4918..6222
                     /locus_tag="PLX80_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            6566..8575
                     /locus_tag="PLX80_10595"
     CDS             6566..8575
                     /locus_tag="PLX80_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY21278.1"
                     /translation="MKKLILIILLILASNSFSQTGYRVSMLANLNEHFTSAGASYSAL
                     WGYTSPDNREYALLGCFTGTAFIDITDTNNIHEVDFLPAPNAFGFGNFWREMKTYSHY
                     AYIVSEDNNSNIQIVDLQYLPDSIRYVGKFILPGHTSTHSISQSGAFLYLNGCNSSFS
                     QGITILDLTNPEVPVLRGKWQDIYVHDSRIINDTIWACNIFDQRVTIINAVNKDNPVT
                     IRNWINNPEPNAPHNIAFNSSRNIAYVTDETTEPTSGKLKVWDVSDQENITYIRSFNA
                     FPFQNSIVHNIEVVGNYAFVAYYSAGIKVLDISNPTNPAEVGWFDTYPENNDAIYDGC
                     WGIYYFNSGKIIASDMTRGLFVLKPNLTNPVSHFPDVKFSVSNLNIMKFDTLRLIDAT
                     EGIPTSWQWTVTGAENKSSALQYPGFAFTQTGDYSVKLKVTNSFGSDSVEMTNAFHVV
                     PIPLNSFSVLSPLGNPSFTIITSPVDTSKVLFSWKKSSADPENIYKLNFRKLQNGTEK
                     FYLSGNNGLDTFALLTKSFLDSMSQSFGLTGDSVLVTFKVRVYSLSDSLNSANQSLII
                     LKRNSVGIQNISQNIPGEFKLHNNYPNPFNPSTNIEFDLPVSGVVSLELYDIAGKKVL
                     DMLNENLSAGKYKYTLNGSNLNSGVYFVKFYAGSFTSVKKILLIK"
     gene            8662..>9003
                     /locus_tag="PLX80_10600"
     CDS             8662..>9003
                     /locus_tag="PLX80_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21279.1"
                     /translation="MDFKIKLIFVSVLTLYFFNTGYTFSQTSVDLNGSRFRGKAEEQT
                     PPDVDRMPIVMNEIINFNNGVISSEVFKKYTVTECKYSAEIDERRMVAVKVVNFNSEC
                     QGVYENENVTLK"
BASE COUNT         2952 a         1529 c         1464 g         3058 t
ORIGIN      
        1 tactaaaata atttttttcc gattagtaac atcaaattca tttgtaacta caaaaaatat
       61 tgtgtaaaaa attatttctt tgagaaatct tatataactg atctcaggat ataaggataa
      121 tggaatggat aatattcggg ctgcaataaa aattaaaaaa attttatcaa gcggtgtctt
      181 tacaaaggta taattttttg ttacaataat ttttacaatc caaagtatga tagcactgct
      241 tgtaaagatc tgcaataatg caattgaaaa aatggcagtt gaaattaaag ctatgattga
      301 aaaccaaata gctttatcaa gataagaatc taaacctttt tctttagata aaaataaatt
      361 cattcagatt tgtttattct tttcagtaat ggatttaaaa atatgagaat ctttcttatc
      421 cagtctggat tttgttcact taatttagca tcaaattccc acttactttc agaaactctt
      481 tttttcataa cttcaggatg ggaaccatca aacttttcta aatttccgag atcatcataa
      541 ttctgaaatc cggaaatatt cttttcagca gtctcacctt cattgtaaag tttttcaaaa
      601 tctcttcttt tatttaaaag tttttccgga ggtctgaccc atccgtaatg atagatctct
      661 gcatctatat ctttcgagtt gagtttgctt ccatcaggat gccgaaaatc caaagcgtct
      721 ccccatgaaa ttatattgtc tttctttctg actatcctga cctctctgat ataccacttg
      781 cggtaattat cctgaacata atcataactt ccataaaaat gtttatacca aaatcttaat
      841 ccttctatat ttggtttatt tagatttaca ctcatagctt ttttcacatt ttcaaaaaat
      901 ttttcatgca atacttcatc tccctgaata taaaaacacc agtcgccggt acatttatca
      961 agtgctttat ttgtttccac tgacaaaacc tctccgcctt tcagagacat gtcccattct
     1021 gattcgaaga tctttatttt tttattatcg atcgctctga taatttcaag tgtgttatca
     1081 tttgatttac ctacattaac aatcatctca tcacaaagcg gaagtataga ttgaattgct
     1141 tctacataag gataggcata gagttcgcca ttcctgacta tagtaaatcc ggagactttc
     1201 atagaattat ttcctgatgt ctgaattgaa gattgtaaag ttttttataa atgctctcat
     1261 ttaataagag ttcatcatga gtgcctgttt caataatttc acctttactt aaaactacta
     1321 tcatgtctgc atttctgact gttgaaagtc tgtgagctat cacaatagat gttcttccta
     1381 tcataagtct ttcaatagcc tgttgcacga gtaactcaga ttcagtatcg agtgaagaag
     1441 tcgcttcatc taaaatcagt atcggtggat ttttcagtaa agctcttgca atggaaagtc
     1501 tttgccgctc acctcccgaa agtttaacac ctctgtcacc tatgacagta tcatatccat
     1561 tttcaaattt ctcaataaat atatgcgcat tggcagcctt tgcagcgtcg ataatctttt
     1621 cgtgagagat atcctgctta ccatatgcaa tattattttt aacactgtca ttaaaaagga
     1681 tagtctcctg tgttacaatg cccatctttt cacgcacaga ttctattgtc agatctttga
     1741 tctcagttcc atcaatttgt aaacttcccg aaacagcatc ataaaatctt ggtatcagat
     1801 ctacaagtgt agatttacct gcgccgctag gacctacaat ggcgatgatc tttccttttt
     1861 caatcgtaag gttcacattc cttaagatca tatcactctt ctcatattta aaagaaacat
     1921 ctttgaattc aatatagctg ctgaattctt tgagttcttt tgcgtcgggt ttatttacta
     1981 tatccggttt tgtatcaagt aatttaaata ctctttctga tgctgcagat ccttctttat
     2041 aactgttgaa catttgaccg aataatttta acgaaggcat catctgaaaa aatactgcga
     2101 ggtaaaaaat aaatgcaccc ggagtcatgt cactgctgcc tttgataatg tcagatccga
     2161 aaataaaaag aatgatcaca attgtaatta caccgatgat ctcagatacg ggagatgcaa
     2221 gagctctttt acggaaaatt tttacaagca gctcgaagta agaataattt tctttttcaa
     2281 atttttgttt ctcatattct tccataccga aagctttgac tactctgact gcaccgagag
     2341 tttcatctaa agtggaggta atatctgaaa ttttttcctg agatttctga ctgctgcgtt
     2401 ttaatgagtt gccgatctta cttaatataa ttgcagttat tggtgccatc agaaatatga
     2461 tgagtgttag tttccagttg aataaaaata atattgtaga aaaaactatg atagtaggcg
     2521 gatccctgaa aaggctgtta agtactgcta tcagagaatc ctttatgatc tgaacatcat
     2581 taatgatcct tgaaataaga tttccttttc gttcatcagt gaaaaagcta agaggaagtt
     2641 ccagataatg atcataaaga tcccttctga tatctttcag aataccctgc tctactacag
     2701 acataaagta ggtttgcaga tagctgaaaa gattttttaa aaagaatgta aggaagataa
     2761 gtatacaaag atatttaagt acatccagct tatcataatt cataagtatc tttgcgaaat
     2821 aaacgataat aaaatctttc agatcaaata tggatgtaat ttctttatca ggaacagtct
     2881 ttggagcatc attgaacagc aggtccataa agggaaagat catcattact gataacagac
     2941 taaatatcac aacaagaatt gtaaaaatgt tagctgttat aagttgtttt aaataaggtt
     3001 ttacatattt aaaaattcgt ttataattat gcatttattt tctgttatcc tttttatttt
     3061 gaatctctat catcttcatg tatgtaagaa ttttcgtgat catgtgaaaa aaggaaacca
     3121 ttagtccata gatcccatct ctgaaaccct gctggaaaaa gaatttcttt ttaaattcaa
     3181 aaaatggttt gaaaaataaa tacgacatac tgatcttttt accgtctgat ttttcaagag
     3241 cagacaatgc agagtaatga ttgatttttt ctgcgaattc tgtaactgat ctgactgtgt
     3301 aatgtaagat atcacttttc aattttgtag tttctccggt cacgttataa ccttcatgta
     3361 ctagtctttc tgaaacagta actttatctc ttttgaataa tctcatctga tgatcgggat
     3421 accatccgca gtgctgtatc catttcccca ggaaaaaact ttttctaggt atgagataac
     3481 catttacatt attactgctg tctaatattg atcttatctc caaagctaac tcttcagtac
     3541 atctttcatc tgcatctaaa gaaaaaatcc attctgaatt tactttactt aatgcaaatt
     3601 tcctctgctt cgaaaatcct tcccattcat tctgaaatac tttgtctgta aatttcttta
     3661 ctatctctaa agtcctgtct ttactaaaac tgtcaattac cactatctca tcacaccatt
     3721 gaatgctctt tatacaatct tcaatgttat tttcctcatc cctgcatata attaatccgg
     3781 aaattctatt catctaatca atcaaattta tcttttccaa tccattcttg caaaaaaatt
     3841 cttttttctt ttttatttca ttaaagtatt ccatattttt ttcttcttca attgtctttg
     3901 gatgatacat atggtactga actgcaagat ttctaacaga tataaatttt gcattgatga
     3961 gtctaagtct gaattcaata tcagaatctt caccaagtcc cggtccttca taattttcat
     4021 taaagccgtt gatctttatc agcagctctt ttggtacgga aaagttacat cccagaatgt
     4081 gttcgtcctg attggttaca atatttctga ctgatcggtt tttaataatt atgccttctt
     4141 ctatattaaa attaaattca ttcttacctt tattaagtga tgaataaatt acctcactta
     4201 atttaagttt ctcatactcc ttattaagta ttgattcttt agtgatcttt tctgaaattt
     4261 tttttgtgag attaactctt cttccacata agacagtatt ctcagctcta ttttcaaaat
     4321 gagcttttac aaaatctgaa tgcggaatac agtcgccgtc aaaaaatatc agatgatctg
     4381 atgaagcatt tctgattgat ccattcagga tcctgttttt tctaaagcca atgtcttcct
     4441 gtgtcagatg tttaacagga aaattatcag atgatttcca tttattgatg agtgatctca
     4501 tttcttcatt catcccatct tcagcaatga tcacttcaaa atctttaaaa ctctgaatag
     4561 aaagtgcatg aaaaataagc tcaagctctt ttactttatt ataaacggaa ataattaatg
     4621 taactttatg catttgatta gcaaaaatat gcattaaaat ttttaagtaa tagttaattt
     4681 taaatttcaa ctttcctcta tataatatcc gtccaaaact ttttattata aaattatata
     4741 aatacatttt ctactgatct ttcttaagcg atcaataaag aaccaatacg agacttagta
     4801 ttgaacagtt tattattatg atatcttcat aattcataat tcttaatttt taattgcact
     4861 atttttttgt cttatgtaaa aaaatcatat ttaatatatt tataaaaaaa taaaactatg
     4921 gcagttaatt taaataaatt cacgatcaaa gctcaggaag cagtgcagac cgcacaggat
     4981 atcgcttcca attattcgaa tcagttcctt gagccggaac atttgcttgc agcgctgata
     5041 caggatccgg aaggaattgt aacttctact tttcaaaagc ttggcgcaaa caaagggatg
     5101 ctgcaggtta agcttggcga gcttttggaa aatctcccta aagttaccgg tgctgtttca
     5161 ggacattccc tttctcctga attaaacggc gttctcgaaa gtgctgtaaa ggaagctgct
     5221 aatcttaaag acgaatatgt ctcgacagag catcttcttc ttggactttc cgatctgaaa
     5281 aaatctcctg tatcggattt tttaaaatct catgacatta caaaaaataa tattctcaaa
     5341 gctctgaaag atgtaagagg tacgagccgt gtatcgggtc agaatcctga agatacttat
     5401 gaagctttaa aaaaatatgg taaaaatctg aatgaacttg caatgaaagg aaaactcgat
     5461 cctgtcatag gaagagatga agaaattaga agagttttac aggttctttc cagacgaact
     5521 aaaaataatc cggtattgat aggtgagccg ggagtaggaa agactgcaat tgctgaagga
     5581 cttgcaatca gaattattca gggagatgtg ccggaaggtt tgagatcgaa acagatagta
     5641 gctcttgata tgggttcact cgttgcaggt gcacagttca gaggtcagtt tgaagaaaga
     5701 ctaaaagcag ttttaaagga agttcaggaa tccgaaggtg aaataatttt atttattgat
     5761 gaattacata cagtcgtagg agcaggtgca gcacagggtt cggtcgatgc ttcgaacatg
     5821 ctgaagccgg cgcttgcaag aggtgagctg cgaactatcg gcgcgactac tctcgatgaa
     5881 tacagaaagt atattgaaaa agatcctgca ctcgaaagaa gattccagcc ggtatttgtc
     5941 ggtgagcctg atgtggaaga tacaatttca atactgagag gattgaagga gaagtatgaa
     6001 gttcatcacg gagtgcggat cacggacggc gcaatcgtag cagcagcaca gctttctgac
     6061 agatacatct cggacagatt ccttccggat aaagcaattg accttgtaga tgaagcggca
     6121 tccaaactca gaattgaaat tgactcaatg cctgaagagc ttgataatct tgacaggaag
     6181 ataaagcagc ttgagattga gaaggaagcg ctcaaaagag aagtttgaaa gagcaattga
     6241 gaattaaaaa ttaggaatta agaattgcat gttaatagtt agaagttaat agttttttta
     6301 aatatagttt tatagtaaat aaaatgcaga gtcaaatgaa ttttgaactt tgcatttttt
     6361 tgtaattatc aaactgaatc tttatctgat cagtaacctt ttcttagttt tggaaaattt
     6421 tttggaagtg agagtataat agtaaacacc gctaatcaat ccgaattaaa tttttattta
     6481 actaccaatg taaaagtgtt gattaaaatc atatagatag ttaagtttaa atacttacaa
     6541 accaccgacc cttatcaatt tcattatgaa aaaactaata cttattattc tgctgattct
     6601 ggcttcaaat tctttttcac aaaccggcta cagagtaagt atgcttgcaa acctaaacga
     6661 acatttcact tcagcaggtg cttcgtattc agctttgtgg ggatatacat ctccggacaa
     6721 cagggaatat gcactgcttg gatgttttac cggaacagct tttattgata ttactgatac
     6781 aaataatatt catgaagttg attttctgcc ggcacctaat gcatttgggt ttggaaattt
     6841 ctggcgggaa atgaaaacat attcacatta tgcatatata gtttctgaag ataataacag
     6901 taatattcaa atcgttgatc ttcagtatct gccagactcg atcaggtatg tgggaaaatt
     6961 tattctgccc ggtcatactt ctactcattc tatatctcag tccggtgcat ttctttatct
     7021 taacggatgc aacagttcct tttcgcaggg tattacaatt ctggatctga caaatcctga
     7081 agtaccggta ttaaggggga aatggcagga tatatatgtt catgattcga gaataattaa
     7141 tgacactatc tgggcttgta atatctttga tcagagagta acaattatta atgctgtaaa
     7201 taaggataat ccggttacaa taagaaactg gatcaacaat cctgagccta atgcacctca
     7261 taatattgca ttcaatagta gcagaaatat agcttatgtt accgatgaaa ctacagaacc
     7321 gacttcagga aaattaaaag tatgggatgt ttccgatcag gaaaatatta catacatcag
     7381 atcattcaat gcttttcctt ttcaaaattc tatagtacat aatattgagg ttgttggtaa
     7441 ttatgctttc gttgcatatt attctgcagg aataaaagtt cttgatatct caaatccgac
     7501 aaacccggca gaagtcggat ggtttgatac ttatcctgaa aataatgatg cgatctatga
     7561 cggctgctgg ggaatttatt attttaattc cgggaaaata attgcctctg atatgacacg
     7621 tggattattt gtacttaaac caaatttaac aaatcctgta tcacattttc ctgatgtgaa
     7681 attttctgta agcaatctga atataatgaa atttgacact ttgagactga tagatgcaac
     7741 ggaaggaatt ccaacttcat ggcaatggac tgtcacaggt gcggaaaata aaagctcggc
     7801 tttacaatat cccggatttg catttactca gactggagat tacagtgtaa agctgaaagt
     7861 cacaaattca tttggatctg attctgttga gatgaccaat gcttttcatg tagttccaat
     7921 ccctttaaat tcattttcag ttttaagtcc tttaggtaac ccgtcattta cgatcattac
     7981 ttcaccggta gacacatcaa aagttttatt cagctggaaa aaatcttcag cagatccgga
     8041 gaatatctat aaacttaatt tcagaaaatt acaaaacggc actgaaaagt tttatctttc
     8101 aggaaataac ggtcttgata cttttgctct tttaacaaaa agttttcttg acagtatgtc
     8161 gcagagtttc ggattaacag gggattcagt tttagttact tttaaagtaa gagtatacag
     8221 cttatcggat tcattgaatt cagctaacca aagtctgatc atattgaaaa gaaattcggt
     8281 gggcatacag aacatctctc aaaatattcc cggagaattt aagctgcata ataattatcc
     8341 gaacccattt aacccatcaa caaatataga attcgatctg cctgtatccg gagtggtaag
     8401 tctggagctt tatgatattg caggaaaaaa ggttcttgat atgctcaatg aaaatttatc
     8461 tgcggggaaa tataaatata ctttgaacgg aagtaatctg aacagcggag tttattttgt
     8521 aaaattttat gcgggaagtt ttacttcagt aaaaaaaata ttgttaatta aataaaaaaa
     8581 taaaattaag taaatatact taatgaattt taacgtactt attgctaaat tgtattgcaa
     8641 taatttaata ataagtaaaa aatggacttt aaaataaaat taatctttgt ttcagtttta
     8701 accttatatt tttttaatac aggttatact ttttcccaaa cttcagtaga tctgaatggc
     8761 agcagattca gaggaaaggc agaagagcag actccgcctg atgtagacag aatgccgatc
     8821 gtgatgaacg aaattattaa tttcaataat ggtgtaatct caagtgaggt ctttaaaaaa
     8881 tatacagtca ctgaatgcaa atattcagca gagattgatg aacgaaggat ggtcgcagtt
     8941 aaagtagtaa attttaattc agaatgtcag ggcgtttacg aaaatgaaaa tgttacacta
     9001 aaa
//
LOCUS       DAORVQ010000138         8917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4329_length_8917_cov_5.960280, whole genome shotgun sequence.
ACCESSION   DAORVQ010000138 DAORVQ010000000
VERSION     DAORVQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_8917_cov_5.960280"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /gene="hutU"
                     /locus_tag="PLX80_10605"
     CDS             complement(<1..1412)
                     /gene="hutU"
                     /locus_tag="PLX80_10605"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQY21280.1"
                     /translation="MSRNIKAPTGNKLSCKSWTTEAALRMLMNNLDSEVAERPEDLIV
                     YGGSGKAARNWECYDKIVESLKVLNDDETLLVQSGKPVAIFKTHKDAPRIMISNSQIV
                     PAWADWDEFRRLEALGLTMYGQMTAGSWIYIGTQGILQGTYETFASCADKHFGGTLAG
                     RFVLTCGLGGMGGAQPLAATLNGAAILGVDVDESRIQKRIDTGYCDVMTHDLDEALQL
                     ILEAKQTKTDKSVGLVGNAAEILPEILKRGIIPDVVTDQTSAHDMLNGYVPMDMPFNE
                     AITLRKSDPQKYQELARKTVVIHCKTMWEFMKKGSVVFDYGNNLRGEAYNNCFKSAFE
                     IPGFVPEYIRPLFCEGKGPFRWVALSGDPEDIYRTDEVIMKAFPDNKPLHKWLKAARE
                     KVHFQGLPSRICWLGYGDRAKAGKLFNDLVRDGIVSAPIVIGRDHLDCGSVASPNRET
                     EKMKDGSDAIADWPILNFGL"
     gene            complement(1536..3053)
                     /locus_tag="PLX80_10610"
     CDS             complement(1536..3053)
                     /locus_tag="PLX80_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21281.1"
                     /translation="MKVTDDLFQLIKSLEQSEKRYFKIFASMHIKDTDNNQYLKLFDA
                     IDKQENYDEDDIKKKFAKEKFIKQIHVAKNYLYNNILKSLRLYHSDRSKVNELMDILR
                     DVQILYDKSLYKQCRKLLEKAKKIAYTYEKHAQILAVIDWEKTLARTSSYSNTDEAEL
                     ISYYNEHNVATEKLINLNEYWNLATKAFMFRKKQGDFRDRNELKKFNSIFENELLTSE
                     KFATSFLSKTFYYNLKGLYYLTNKDNKNLLIHCEKIVRLLEENPVLLKEDNYIASLYN
                     LLLVEIELKKFSDALKTINKIRSIKTDSSTLQARIFVTSYDTEINLYMKSGEFRKGEK
                     LVVEIDEGLVKMKSKINKESKILFAYNIAYIYFGLKKYDESLIRINEIINDKELKIRE
                     DIQCFARILNLLIHYELGNYDLIEYIMKSTKRYLSSKNKFNKFESITLGQIRKLISEK
                     TEDKNYLFIEWKKELDKISDDIMEINALEYFDLISWIDSKLTGESFIDLLKRKNT"
     gene            3274..4194
                     /locus_tag="PLX80_10615"
     CDS             3274..4194
                     /locus_tag="PLX80_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21282.1"
                     /translation="MISGYIFSQTINAPGDLEAEPEDSSYIKVKWDDNSNNEEGFYVE
                     RSLTADTSVVWETIGSTGQNNRLFADYWVTNGVEYFYRVYAYAGNIRSAYSNIASTVA
                     EIDTSIIPRAPTDLLIAEITETSVTITWQDNSYNESGFIIARREINEIEFRYIDTVQT
                     DVLTYQEVGLTPDNIYLYKVCSYNLFGLSDFTNTVSARTNKSTSIINTFSEIPDGFYI
                     SENFPNPFNPETKIKFAIAENSTVNMEIYSIAGKKIETLVNSELHAGTYEVIWNASEF
                     ASGVYFLRTRVIYNGGGSVFSKFNKMILNK"
     gene            4211..4858
                     /locus_tag="PLX80_10620"
     CDS             4211..4858
                     /locus_tag="PLX80_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21283.1"
                     /translation="MKNSKLFFAIMFMFLISFSAVNSNATIVNITASNFSFTPVLVNV
                     TVGDTVKWTWTSGSHTTTCDGQQGTVLPNGAASWDAELSSNAVTYMYVITVSGNYQYV
                     CTPHAPDMSGTINAVVSSISQINEIATGYKLSQNFPNPFNPETKINFSIPSSGFVDLK
                     VYNAAGQNVASLVNQRLSSGTYNVNWNASDVTSGVYFYTLKTENFTETKKMLLIR"
     gene            4895..5332
                     /locus_tag="PLX80_10625"
     CDS             4895..5332
                     /locus_tag="PLX80_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQY21284.1"
                     /translation="MSKENLNRRDFLKNSVKTIAIGGLALSSLDIKKLIASAPVEIDS
                     TQAKVINLSEYPALESTGGHAMINDSTIVIRTGSSKFIALKTVCTHKKCDVDYLGGEG
                     FECPCHGSTYSQSGKVLSGPAKNNLKSYKTTYNSENNTLTINM"
     gene            5575..6012
                     /locus_tag="PLX80_10630"
     CDS             5575..6012
                     /locus_tag="PLX80_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQY21285.1"
                     /translation="MNIVKFKKDSDLLNDTFRSVFTSPLFRQVDNSNPNTNFIPKVRI
                     SEDKDNFFINMEIPGMKKEDVKISLEDNVLSINGVRKHEKKTEETNLITNEIYFGEFS
                     RSFNLSKEIKTDSIDAEYKDGLLNITLPKVEEAKPVVKEINIK"
     gene            6130..8103
                     /gene="dnaK"
                     /locus_tag="PLX80_10635"
     CDS             6130..8103
                     /gene="dnaK"
                     /locus_tag="PLX80_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQY21286.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNDPVVIPNAEGARTTPSAVAFAK
                     TGDRLVGDPAKRQAVTNPQNTIFSIKRFMGRRFDEVTEEIKKVPYKVIKGEGDTARVE
                     IDDKNSGQKRIYSPPEISSMILQKMKKTAEDYLGQPVTEAVITVPAYFNDAQRQATKD
                     AGKIAGLEVKRIINEPTAAALAYGLDKKKANEKVVVYDLGGGTFDVSILELGDGVFEV
                     KSTHGDTHLGGDDFDQVLIDYLADEFKKKEGIDLRTDPMALQRLKEAAENAKKALSSS
                     SQTEINLPYITATAEGPKFLLENITRAKFESLIGDLVAKTEGPCKKALEDSGYSMNDI
                     DEVILVGGSTRIPLVQETVKKLFGKEPNKSVNPDEVVAIGAAIQGGVLSGDVQDVLLL
                     DVTPLSLGIETLGGVMTKLIEANATIPTKKSQVFSTAADNQPSVEIHVLQGERAMATD
                     NRTIGRFHLGGIPRAPRGTPQIEVTFDIDANGILNVAATDKGTGKQQQIKITHSSGLS
                     DAEIEKMKTDAKENEGEDAKKKEMIEMKNQADAMVSQGEKQLKEFEEKLDAETKSKIQ
                     MSLDRLKEAAKSDNISTLTTAIEEYNTVWNEASSQMYSQSTGADGQQTGEAGSQPGGE
                     EAKTEDANVENADFEVVEDENKDEDKKDEDKKD"
     gene            complement(8164..>8917)
                     /locus_tag="PLX80_10640"
     CDS             complement(8164..>8917)
                     /locus_tag="PLX80_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HQY21287.1"
                     /translation="NDYPKIEKVYEIENDGAFYYGPFRSGMTVNELLKEIDGEFLLRK
                     CENKKIKASKDHSVCMYHEIGRCKAPCNLTQSKSEYDNEVMKVHDFITESENNSVQNL
                     YEMRMMEFSESMDYESAAFIRDRLKDIRKVMSYQKVITSAINGKKLIIKCENESAKEF
                     FFIHNGKLAKTYTFTNENQTDQNEILEELTESTEYLFFSLSKFVKHKFNPVELDEIKV
                     ISNWLALNKDKNSVFEIREDHSLKDVMKFFIA"
BASE COUNT         2954 a         1498 c         1555 g         2910 t
ORIGIN      
        1 tgaagtccaa aattcagaat cggccagtca gcaattgcat cagaaccgtc tttcattttt
       61 tctgtttctc ggtttggaga agctacactt ccgcagtcaa gatgatctct gccgataact
      121 attggcgcag aaacaattcc atctctgact agatcattaa ataatttccc tgcctttgct
      181 ctgtctccgt aaccaagcca gcatatcctc gaaggtaatc cctgaaaatg aactttctct
      241 ctggctgctt tcagccattt gtgtaatggt ttattatcag gaaatgcttt cataataact
      301 tcatcagttc tgtaaatatc ttcaggatct ccggatagtg caacccatct gaaaggacct
      361 tttccttcac agaataatgg tctgatatat tccgggacaa atccgggaat ttcaaaagcg
      421 gatttgaaac aattattata agcttcacca cgcagattat ttccataatc aaaaacgaca
      481 gaacctttct tcataaattc ccacatagtt ttgcagtgaa tcacaacggt ctttcgtgca
      541 agttcctgat atttttgcgg atcggatttt ctgagtgtga tggcttcatt aaaaggcata
      601 tccattggaa cgtaaccgtt cagcatgtca tgagcagagg tttgatcagt gacgacgtca
      661 ggtataattc ctcttttgag aatttcggga agaatttcgg cagcgtttcc gacgaggcca
      721 accgatttat cggtttttgt ctgcttagcc tccaggatta attgcagtgc ctcatcaaga
      781 tcatgagtca tgacatcgca gtatcccgta tctattcttt tttgaatccg gctttcgtcc
      841 acatccactc cgaggatagc agctccgtta agtgtagctg ccagaggctg agctccgccc
      901 attccaccaa gtccgcaagt aagtacaaat cgtccggcaa gtgtaccgcc gaaatgtttg
      961 tccgcgcatg atgcaaatgt ttcataagtt ccctgtaaaa ttccctgtgt gccaatatat
     1021 atccatgaac ctgctgtcat ctgaccgtac attgtaagac caagtgcttc tagccttctg
     1081 aactcatccc agtcagccca tgccggcact atctgagaat ttgaaatcat tattcttgga
     1141 gcatctttgt gtgttttaaa aattgcaaca ggttttcctg attgtactaa aagagtttca
     1201 tcatcgttaa gaacttttaa agattctaca attttatcat aacattccca gttccttgca
     1261 gcttttccgc tgccgccata tacgataaga tcttccggtc tttctgctac ttctgaatca
     1321 agattattca tcagcattct caatgctgcc tcagtggtcc agctcttaca tgaaagttta
     1381 ttaccggtag gagctttaat gtttctgctc attatttaga tttacttaaa attttttatc
     1441 aaaataaaaa ttctgttcat taaaatatat taataaagta tcaccctttt attctttatt
     1501 tatacctgct ttaaaagtta gttcaaaaaa tccttttaag tatttttcct ttttaacaga
     1561 tcaataaagg actcccccgt aagcttactg tcaatccatg aaataagatc gaaatattct
     1621 aaagcattta tttccattat gtcatcagat attttatcta attctttttt ccattctata
     1681 aacagatagt tcttatcttc tgttttttca ctgataagtt ttctgatctg acctaatgta
     1741 atggattcaa atttattgaa tttgttttta ctagaaagat accttttggt tgatttcata
     1801 atatattcta taaggtcata atttccgagt tcataatgta taagcagatt taaaattctt
     1861 gcaaagcatt gaatatcctc tctgattttt aattccttgt catttatgat ctcatttatt
     1921 ctgatcagag attcatcata tttttttaat ccgaaataaa tatatgctat attgtaagcg
     1981 aataaaattt tggattcctt gttgatctta cttttcattt taacaagacc ttcatcaatc
     2041 tcaacaacaa gtttttcgcc tttacggaac tctccggatt tcatatataa gtttatttcc
     2101 gtatcatatg atgtcacaaa tatcctcgcc tgtaaagtcg aagagtcagt cttgatactt
     2161 cttattttat ttattgtttt cagtgcatca ctaaattttt ttaattcgat ctcaaccaat
     2221 aaaagattat atagtgacgc aatgtaattg tcctctttta aaagtacagg attttcttca
     2281 agcagtctga ctattttttc acaatgaatt aaaagatttt tattgtcttt atttgtcaga
     2341 taatataatc ctttaaggtt gtaataaaat gtttttgaaa gaaatgaagt agcgaatttt
     2401 tcagatgtca acagttcgtt ttcaaagatg ctgttgaatt tttttaactc atttctgtct
     2461 ctaaaatcac cttgtttttt tctaaacata aatgccttag tcgcaaggtt ccagtattca
     2521 tttaagttaa ttagtttttc agtagcaaca ttatgttcat tataatatga aataagttca
     2581 gcctcatcgg tatttgagta agagcttgtt ctggcaaggg ttttttccca gtctattact
     2641 gcaagtatct gagcatgctt ttcgtaagtg tatgctattt ttttagcttt ctcgaggagt
     2701 ttcctgcatt gtttataaag agatttatcg taaaggatct gtacatctct gagaatatcc
     2761 attagttcgt tgactttgga tctgtcagaa tgatataaac gcagactttt taaaatgtta
     2821 ttgtatagat aattttttgc aacatggatc tgcttaatga atttttcttt tgcaaatttt
     2881 tttttgatat catcttcatc gtaattttcc tgtttatcaa tagcatcaaa aagtttcagg
     2941 tactgattgt tatcagtatc tttaatgtgc attgaagcaa aaattttgaa gtatctcttt
     3001 tcggattgct caagtgattt aataagctgg aaaagatcgt cggtaacttt caagaataat
     3061 ttcccgtttg tttagttttg tatgaatttc aaaggattta aaataagtaa aataaatctg
     3121 tattttaatc taaattagaa tcctacggat atccttattt ttgtttgtgt ttcatatgca
     3181 tatattatat ttttgaataa ctaatttttc tgaaaaatga ttatcaccaa aaaattattt
     3241 ctttatttct tattaataac tttaaatctt gttttgatct caggttatat tttttcacag
     3301 actataaatg ctccgggaga tcttgaagcc gaacctgagg attcaagtta tataaaagtt
     3361 aaatgggatg acaattcaaa taatgaagaa gggttttatg tagaaagatc tttgactgct
     3421 gatacaagcg tagtttggga aacaatagga agcacaggtc agaataacag gctctttgcg
     3481 gattactggg taacaaatgg agttgagtat ttttacagag tgtatgctta tgctggtaac
     3541 ataagatcag catattcaaa tattgcttct acagttgctg aaattgatac atcaataatt
     3601 cctcgggcac caactgatct gttaatagct gaaattacgg aaacatctgt cacaattaca
     3661 tggcaggata attcctataa tgagtccggt ttcattattg cccgaagaga gatcaatgaa
     3721 attgaattca gatatattga tactgtgcag actgatgtac ttacttatca ggaagttggt
     3781 ctgacaccgg acaatattta tttgtataaa gtctgctcat acaatttatt tggtctgtcg
     3841 gattttacaa atacagtttc tgccagaaca aataagagta ctagcataat aaatactttt
     3901 tctgaaatac ctgatggatt ttatataagt gaaaattttc cgaatccgtt taatcccgaa
     3961 acaaaaatta aatttgcaat tgctgaaaat tcaacagtaa atatggaaat atacagcatt
     4021 gccggtaaga aaattgaaac tcttgtaaac agtgaattgc atgcaggaac ttacgaagtg
     4081 atctggaatg catcagaatt tgcgagcggt gtttattttt tacgaacccg tgtaatttat
     4141 aacggcggcg gatcagtatt cagtaaattc aataaaatga ttttaaataa ataaactaaa
     4201 aatatattaa atgaaaaatt caaaattgtt ttttgcaata atgttcatgt ttttaataag
     4261 cttcagtgct gtaaattcta atgcaacaat agtaaatatt acagcttcaa atttttcttt
     4321 tacccctgtg ttagtaaatg taacagtggg agatactgta aaatggacat ggacaagtgg
     4381 aagtcatact acaacatgcg atggtcagca aggaacagta cttcccaacg gagctgcttc
     4441 ctgggatgca gaattaagca gcaatgctgt tacatatatg tatgtaatca cagtttccgg
     4501 taattatcag tatgtatgta cacctcatgc gccggatatg tcaggtacga taaatgcggt
     4561 agtttcttct atttcacaga ttaatgaaat tgcaaccggg tataaacttt cacaaaattt
     4621 tccaaatcca ttcaatcctg aaacaaaaat aaatttctcg attccatcat ctggttttgt
     4681 cgatctgaaa gtgtataatg cagcaggtca gaatgtagct tcacttgtaa atcaaagatt
     4741 atcatcaggt acttataatg taaactggaa tgcttcggat gttacaagcg gagtttattt
     4801 ctatacctta aagacagaaa actttactga aacaaaaaaa atgctgctga tcagataatt
     4861 tattagaatt ttaaaaaact aaatatttaa aataatgagc aaagaaaatt taaacagaag
     4921 agacttttta aaaaactcag tcaagacaat agcaatcggc ggtctggcat taagttcact
     4981 tgacataaaa aaactgatcg catctgcacc agtggaaata gattcgactc aagcaaaggt
     5041 tataaatctt tcagaatatc ctgcacttga aagcaccgga ggtcacgcta tgatcaatga
     5101 ttcaacaatt gtaataagaa ccggcagcag caaattcatt gcattaaaga ctgtctgtac
     5161 acataaaaaa tgtgatgtcg attatcttgg aggagaaggt tttgaatgtc cgtgtcatgg
     5221 ctctacttat tctcagtcag gaaaagtttt aagcggacct gcaaaaaata atcttaaaag
     5281 ttataaaacc acttataatt ctgaaaataa tactctgact ataaatatgt aattttttta
     5341 attggtattg gtgtaatcag aaaatccgga tcctgtttta aaaagggatt cggatttttc
     5401 aatttattga aatatttttc aaaccagtgc accatttttc aatttattga aattattttc
     5461 atttaattat ttgtcaattc ttcattctat agccaaaaac gtattattcg tcatggcacg
     5521 ataattgaaa taatacgaag tgtattaata aattaaaata caggagaaaa gaaaatgaac
     5581 atagttaaat tcaaaaaaga ctcagattta ttaaatgata cattcagatc agttttcacc
     5641 agtccattat tcagacaagt agataattct aatccaaaca caaattttat tccaaaggta
     5701 agaatctcgg aagataaaga taatttcttt atcaatatgg aaatccccgg aatgaaaaag
     5761 gaagatgtaa agatttctct tgaagataat gtgttaagta tcaatggtgt caggaaacat
     5821 gaaaagaaga ctgaagaaac aaatctgatc acaaatgaaa tttattttgg tgaattcagc
     5881 agaagtttta acctttcaaa agaaattaaa acagattcaa tcgatgctga atacaaagac
     5941 ggattgctta acatcacttt accaaaagta gaagaagcaa agccggttgt aaaagagatc
     6001 aatattaaat aagtaaaaca atattttaat taacaataaa taaagggtga gaaaagctcg
     6061 ccctttattt ttttcatgta aagtctcatt gactatacat taaaattaaa aaaaaggtaa
     6121 aataatatta tgggtaaaat cattggtata gatttaggaa cgactaattc ctgtgtagct
     6181 gtaatggaag gaaacgaccc tgtagtaatc ccaaatgcag aaggagcaag aacaacaccc
     6241 tctgccgttg cttttgcaaa gacaggagac aggttagttg gcgatcctgc aaaaagacag
     6301 gctgttacaa atcctcagaa tacaatattc tccataaaaa gatttatggg aagaagattt
     6361 gatgaggtga cagaagaaat aaaaaaagta ccatacaaag ttataaaagg tgaaggtgat
     6421 acagcaagag ttgagataga tgataaaaat tcaggacaga aaagaattta ttctccgccg
     6481 gaaatttcat ctatgattct tcagaaaatg aaaaagactg ccgaagatta tctcggacag
     6541 ccggttactg aagctgttat cacagttcct gcatatttta atgatgcaca gagacaggct
     6601 acaaaagatg ctggtaagat cgcaggtctt gaagtgaaaa gaattatcaa tgagccgaca
     6661 gcagcagcgc ttgcttacgg acttgataaa aagaaagcta atgaaaaagt ggttgtttat
     6721 gatctaggtg gtggaacgtt tgacgtttcg atccttgaat taggtgatgg tgtatttgaa
     6781 gttaaatcta ctcatggcga tactcacctc ggtggtgatg acttcgatca ggtattgata
     6841 gattatcttg cagatgaatt caaaaagaaa gaaggcattg atctcagaac agatccaatg
     6901 gctctgcaaa gattaaaaga agctgctgag aatgcgaaga aagcattgtc ttctagttca
     6961 caaacagaaa taaatcttcc ttacattaca gcaactgcag aaggtccaaa attccttctt
     7021 gaaaatatta caagagcaaa atttgaatct ctgatcggtg acctcgtagc aaagacagaa
     7081 ggtccttgta agaaagctct tgaggattca ggatacagca tgaatgatat cgatgaagtt
     7141 attctcgtcg gcggatctac aaggattccg cttgttcagg aaactgtaaa aaagcttttt
     7201 ggaaaagagc cgaataaaag tgtgaatcct gatgaggtag ttgctatagg agctgcgatt
     7261 cagggtggag ttttatcggg tgatgtacag gatgtattac tacttgatgt aacaccgctt
     7321 tcacttggga ttgaaacact tggcggagtc atgacaaagc ttattgaagc taatgcaaca
     7381 atacctacaa agaaatcaca ggtattttcg actgctgcag ataatcagcc ttcagttgaa
     7441 attcatgtac ttcaaggtga aagagcaatg gccactgaca acagaactat cggaagattc
     7501 catctgggag gaattccaag agcaccaaga ggaacgcccc agatcgaagt tacatttgat
     7561 attgatgcaa acggtattct gaatgttgcg gctactgaca aaggaaccgg taaacaacag
     7621 cagatcaaga tcactcattc ttcaggtctt tctgatgcgg aaattgaaaa aatgaaaaca
     7681 gatgctaaag aaaatgaagg tgaggatgca aagaaaaaag aaatgatcga aatgaaaaat
     7741 caggctgatg ctatggtatc acagggtgaa aagcagctta aggaatttga agagaagctt
     7801 gacgctgaaa ctaaatcaaa gatccagatg tctcttgaca gattaaaaga agctgccaaa
     7861 agtgataata taagcacttt gaccactgca attgaagaat acaataccgt atggaatgaa
     7921 gcttcatctc agatgtattc gcaatcgaca ggtgcagacg gacagcagac aggggaggct
     7981 ggttcacaac ccggcggtga agaagcaaaa actgaagacg ctaatgttga gaatgctgac
     8041 tttgaagtag tagaggatga aaataaagat gaagataaaa aagatgaaga taaaaaagat
     8101 taaaatttaa ctgtatcaaa tataataaaa acctgcttca atccaaaaga agcgggtttt
     8161 tttttatgcg ataaaaaatt tcattacatc tttaagtgaa tgatcttccc tgatttcaaa
     8221 tacactgttc ttgtctttat ttaatgccag ccagtttgaa attactttaa tttcgtctaa
     8281 ttcaaccgga ttgaatttat gctttacaaa cttactcaat gaaaaaaata gatattcagt
     8341 ggactctgtt aactcttcca gtatctcgtt ttgatccgtc tggttttcat ttgtgaatgt
     8401 atatgtcttg gcaagtttgc cgttatgtat gaagaaaaat tctttcgcag attcgttctc
     8461 acattttata attagctttt ttccgtttat tgccgaagta attactttct gatagctcat
     8521 tacctttctg atatccttta atctgtctcg aataaatgct gcactttcat aatccatgct
     8581 ttcagaaaat tccatcatcc tcatttcata aagattctgt acagaattat tttctgattc
     8641 ggtaatgaaa tcatgtactt tcataacttc attgtcatac tcagatttac tttgtgtcag
     8701 attacacgga gctttgcatc tgccgatttc atgatacatg cagacagaat gatccttaga
     8761 tgctttgatc tttttgtttt cacatttacg aagtaaaaat tcaccgtcaa tttcctttaa
     8821 aagttcattc actgtcattc cgcttctgaa tggtccataa taaaacgcac cgtcattttc
     8881 tatctcatat actttctcga ttttcggata gtcattc
//
LOCUS       DAORVQ010000139         8864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4380_length_8864_cov_5.065615, whole genome shotgun sequence.
ACCESSION   DAORVQ010000139 DAORVQ010000000
VERSION     DAORVQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_8864_cov_5.065615"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1059
                     /locus_tag="PLX80_10645"
     CDS             106..1059
                     /locus_tag="PLX80_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015191691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQY21288.1"
                     /translation="MPNLKHRLKVIKEITYKTYDQFLDDKGFKMAAALSYYAAFSLGP
                     LLIICISIAGFIFGEEAVRGELSSQITYLIGKEGAEMVEIIIKGASNLTTGFIAGIIS
                     LILLVLGSLGVFVELQESLNIIWGVEPKPGRGIWGFLKTRVTSFSMVIATGFLMMISL
                     IINTFIGLLYKLLGEYVNPAFPATEIINLVSSFIIITLLFAFMFKYLPDVIISWRYVW
                     IGAVITSILFSVGKYLIGLYLGNSSYSSTYGAAASFIIIFIWIYYSGIILFLGAEFTH
                     VYRSRYSNSKIEPDADGIIVPKVSELIKKSMEESLSKTKNK"
     gene            1203..3200
                     /locus_tag="PLX80_10650"
     CDS             1203..3200
                     /locus_tag="PLX80_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQY21289.1"
                     /translation="MISLNNISMRYGSKVLFDDVSLRVAGKDRISLVGSNGAGKSTLL
                     KVIAGLITSDEGEVAISKHTTVGYLPQEGIHFLGKSLYDEVYSAAGDINLIQEEMHQI
                     ENEMSEFEDTASDEFMDLINEYGELQERFQLLDGFRLKSKIEKILIGLGFFETDFKRM
                     TNEFSGGWQMRIALAKLLLTNPSILLLDEPTNHLDIESLIWVENYLINYQGAIVLVSH
                     DRSFLDNISQRTVEISMGNVTEYSGNYSFYKTEKEVRKELLGNQFNNQQQYLKQQEKF
                     IERFRYKSSKAKAVQSRIKQLDKVDWIEIEDEESTVKFNFPPATHSGKTSIELSGITK
                     SYDNVFNVLENIDLIIGRGEKIVLFGVNGAGKSTLTRIIAGNEKINEGDIKLGHLAQI
                     KFYAQNQAEELNPELTVLETMEQAASGDILKNLRTILGSFLFRGDDVFKKVKVLSGGE
                     KSRLALAKMLIEPSNFLILDEPTNHLDMRSKEVLMNALNKYDGTVLIVSHDREFIDGI
                     VDKVIEVKNKKIKTYIGNASEYLKEKESEFLKGEASQKKTNNVKVRTENNQKPDKSSE
                     KEYKNLKKELNKKIEPVKKEIKTLEESIASFEKEIAAIEKLMAGEDFYKGNHNVLEVT
                     NKYNTIKEKLKNILSQWEKRSKELSDLENQLTNLNQNNITV"
     gene            3197..3955
                     /locus_tag="PLX80_10655"
     CDS             3197..3955
                     /locus_tag="PLX80_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQY21290.1"
                     /translation="MKKIINSSILIVCLLFLSSHQISAQINFDPKLNFKNSNNNSTEL
                     KKKDHETAKKYHQKLTGEFFGASIDFQIGYGTTSPNVTEKNNSKDASTESQGGFTFGA
                     LVNLNLFGLLDLTTGIDLINKKYEYGIPYVSTNPTEGVDSVVNSVKNQYLNIPLNINY
                     GGMVSEKVGLSFSGGPYFGFLLNPESAVSGFKDFDFGLNGIITGNYMLNPFTSIILGT
                     KLQYGGLNNLLSSGNIESVSLTNWDVFTGLRFGF"
     gene            4027..4788
                     /locus_tag="PLX80_10660"
     CDS             4027..4788
                     /locus_tag="PLX80_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21291.1"
                     /translation="MKSYKFVNILFILFSVVFTFSSCGKESDNSNEQFGSDNSKGNES
                     VANKVIDDSDISDKSNLADGISDLKFGKGLVPSFLKDENQIIEAVSWNDKLGENLIAI
                     TLTDERIIKGDERIKELFIYHYIVKNTSSEVLWKSYDYVKDCPFDITLEFIDSSLTVT
                     DLDGDDIAETSFMYKLSCRSDVSPDDLKLIMHEGQDKYAIRGEMILKINDEDPSPGSM
                     IIDDSFNKTKGIFKDFAIEQWNKFNKVFIGEKYEK"
     gene            4778..5428
                     /locus_tag="PLX80_10665"
     CDS             4778..5428
                     /locus_tag="PLX80_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21292.1"
                     /translation="MKSKIIFSKLFIFVALASMIIVTGCSGSKDTSDEETEYAEHNEH
                     TEKDSEVNVKSEESGSYILNIAEAEQKNEMYKIEVDSTYLYWLDNYLLILNGSTKCNI
                     FALNVLKKAGYKTPEVNALSRDLFDTLNFNDILPIVGVNDVSDAMPGDLVIWNGHVIL
                     FESEIELNGQSYAMAYWAGTKNPDNGDNILNNVCYGKYKLNGNYVVRRPLKKLYPD"
     gene            complement(5432..6034)
                     /gene="coaE"
                     /locus_tag="PLX80_10670"
     CDS             complement(5432..6034)
                     /gene="coaE"
                     /locus_tag="PLX80_10670"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQY21293.1"
                     /translation="MIRKKKLMIGVTGGVGSGKTEVCKLLAKRGFKVLYSDTIAKNLY
                     VTNKKLVTEIVKVFGKDIVNYKGKINLPKLKDKIFASKKNYSAINKIVHPVVIKEIKG
                     FLKKNNYDIVIVESALLFESGFDESMDYIIMVYSKKKERVDRLKIRDESTLKSITELM
                     KFQIDERKKMEMSDFVIINNKTLKDLKIQVDFLSKILKAL"
     gene            complement(6067..7722)
                     /gene="rny"
                     /locus_tag="PLX80_10675"
     CDS             complement(6067..7722)
                     /gene="rny"
                     /locus_tag="PLX80_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQY21294.1"
                     /translation="MDLELYILLPILVALCLLTFFLGWYINSRSNKTKIEGADNVAKK
                     ILSDAESKSKDKIEIAEKRSKEILSDAEKASQTLKKEKLLEVKDEFLKKKQQHEEEVK
                     DREKDLVVDEKRIKIEKDNLETVKSELSKLEIKLNKSVELSDSKTKEAENKISESEKL
                     ILMNSEILEEQRNKLQRISGFSEEEARKVLFEKLINDAKIEAAQKIQEIREETKLESN
                     RISKNIIIQSIQRTASDHSVETTVSVLQINSDELKGRIIGKEGRNIRAFEAVTGVDII
                     VDDTPEAIIISGFDPFRREVARVSMERLIADGRIHPARIEEIVEKVQKELTEELIRVG
                     ENTLIEMGIQGVHRDIITLIGRMKYRSSYGQNVLHHSMEVAHLASIMAAELGLDAQMA
                     KRAGLLHDMGKTIDRNIEGPHAILGYEVAKRCKEHPIICNAIGAHHDEMPMEHPISIL
                     VQAADSISGARPGARRESIEAYSKRLEKLENIAKSFEGVVKTYAIQAGREVRVIVEQD
                     KVDDTLQDELAADIAHKIQEEMEYPGQIKVNVIRERRSISYAK"
     gene            complement(8106..8405)
                     /locus_tag="PLX80_10680"
     CDS             complement(8106..8405)
                     /locus_tag="PLX80_10680"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQY21295.1"
                     /translation="MSQKEIRVKIFNNEYNLQGDSTEKVERISDFVDGIMNRLNSESP
                     NQSEETIAVVSALNIAEEYFKEKDIKLDLEKDYSDLLDELISKSNSISDMIDDNL"
     gene            complement(8459..8851)
                     /locus_tag="PLX80_10685"
     CDS             complement(8459..8851)
                     /locus_tag="PLX80_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21296.1"
                     /translation="MNLNNQETTKDFFKDQKNVLRVSLDDLFSKIKSLISKYKDLKNE
                     NNLLKENLKNLNLKISELKLQHTKLNTEIINRDKENSDLKNVVLNSGDNKIPMKDKDY
                     AKTRIQDLITRIDTHLDQYDNDGEYRDN"
BASE COUNT         2908 a         1377 c         1477 g         3102 t
ORIGIN      
        1 ttgaaaaata gagatttaat attaaaatga attttttaaa aaattcaacc gcgttgatag
       61 aaaaaaatat gaaattatta tctgataatt caattaagga ttttcatgcc aaatcttaag
      121 cacagattaa aagtgattaa ggaaattact tataaaacct atgatcagtt tttagatgac
      181 aaaggtttta aaatggctgc agcattatca tattatgctg cattttctct tggtccgtta
      241 ctgattattt gtatttctat agcaggattc atattcggtg aggaagcagt cagaggtgaa
      301 ttgtcttcac agataacata tcttatcggc aaagaaggag cagaaatggt ggagatcatc
      361 atcaaaggtg catccaattt aacgacagga tttattgcgg gtattataag tctgatctta
      421 ttagttttag gatctcttgg tgtatttgta gaattacagg aatcactcaa tattatatgg
      481 ggagtcgagc caaaacccgg aagagggatc tggggatttt taaaaaccag agtcacatca
      541 ttttctatgg taatcgcgac tggctttcta atgatgatat cacttataat aaatactttc
      601 attggtttat tatataaact acttggagaa tatgttaacc cggcttttcc cgctacagaa
      661 attataaatc ttgtgagttc atttataata ataaccttac tttttgcttt catgtttaaa
      721 tacctgcctg atgttatcat ctcctggagg tatgtctgga ttggtgctgt aataacttct
      781 atactttttt ctgtcggaaa atatttgatc ggcttgtatc ttggtaacag ttcatacagc
      841 tctacatacg gagcggctgc ttcttttatt attatattta tctggatcta ttattcagga
      901 ataatattgt ttttaggtgc ggaatttact catgtttaca gaagcagata cagtaattca
      961 aaaattgaac ctgatgctga cgggatcatt gtgccaaaag tatctgagct gataaaaaaa
     1021 tcaatggaag aaagcttaag taagactaaa aataaatgat accttaatcc gatgcaattt
     1081 cctcaattat aaaaacgaat tcaattttgc cccatatatt aatttatctg aaaggttttt
     1141 taaatctttt ttacatttaa attaccactt aatttaagga tgtaaatcac taaattgcac
     1201 ccatgatttc tttgaataat atttcgatgc gttacggctc aaaagttttg tttgacgacg
     1261 tatctttaag agtagcaggt aaagacagaa tttcacttgt cggatctaac ggtgcaggta
     1321 aatccacatt acttaaagta attgcaggtc tgataacatc agatgaaggt gaagtcgcaa
     1381 tttcaaaaca tactacagta ggatatcttc cacaggaagg aattcatttt ctggggaagt
     1441 cactttatga tgaagtgtac agtgctgccg gtgatataaa tctgatacag gaagaaatgc
     1501 atcagatcga gaatgagatg agtgaatttg aagatactgc ttcggatgaa tttatggatc
     1561 tgataaatga atacggcgaa cttcaggaga gatttcaatt gcttgacgga ttcagactaa
     1621 aatccaagat agaaaaaatt cttattggtc tcggattttt tgaaactgat ttcaaaagaa
     1681 tgactaatga attcagcggc ggatggcaga tgagaattgc actggcaaaa cttttgctga
     1741 ctaatccttc tatacttctt cttgacgaac ctacaaacca tctcgatatt gaatcactta
     1801 tctgggtaga aaattatctg ataaattatc agggagcaat agtactggta tctcatgaca
     1861 ggagttttct tgataacatt tcccagcgga ctgtagaaat ttcaatggga aatgtaactg
     1921 aatattctgg taactattca ttctataaaa cagaaaagga agttagaaaa gaactacttg
     1981 gaaatcagtt taacaatcag cagcaatatc tgaaacagca ggaaaaattt attgaaagat
     2041 tcagatataa atcttctaaa gcaaaggcag ttcagagcag gataaagcag cttgacaaag
     2101 tcgactggat agaaatagaa gatgaagaat caactgttaa atttaatttt ccgccggcta
     2161 cacattccgg aaaaacctct attgagcttt cgggtattac aaaaagttat gataatgtat
     2221 ttaatgttct tgaaaatata gatctgataa tcggcagggg agagaagata gttttatttg
     2281 gtgtaaacgg agccggtaaa tctacactta cacgtataat tgcaggtaat gaaaaaatca
     2341 atgaaggtga tataaagctc ggtcatcttg ctcagataaa attttatgct cagaatcagg
     2401 cggaagaatt gaatccggaa ctgaccgttc tggaaacaat ggaacaggct gcttcaggag
     2461 atattctgaa aaatctcagg acgatacttg gtagtttttt attcagaggt gatgatgtgt
     2521 ttaaaaaagt gaaagtactt tcaggcggag agaaatcaag acttgctctt gctaaaatgc
     2581 ttatagaacc ttctaacttt ttaatacttg acgagccgac aaaccatctt gatatgagat
     2641 caaaggaagt gctgatgaat gcactgaata aatatgacgg aactgtactt atagtttctc
     2701 atgacagaga attcatagac ggaatagttg ataaagtaat tgaagtaaaa aataaaaaaa
     2761 taaagacata tataggaaat gcaagtgaat atctaaaaga gaaagagagt gaatttctta
     2821 aaggtgaagc ttctcaaaaa aaaacaaata atgtaaaagt aaggacagag aataatcaga
     2881 agccggataa atcttctgaa aaggaatata agaatctgaa aaaggaatta aataaaaaga
     2941 tagagcctgt taaaaaggaa ataaaaacac ttgaggaaag tatcgcaagt tttgaaaaag
     3001 aaattgctgc tatagaaaaa ctgatggctg gtgaagattt ctataaaggt aaccataatg
     3061 tcttagaggt tacaaataaa tataatacta ttaaagaaaa attaaaaaat attttatccc
     3121 agtgggaaaa aagatcaaaa gaactttcag atcttgaaaa ccaattaact aacttaaatc
     3181 aaaataacat aacagtatga aaaaaataat taactcatct atattaattg tctgcctttt
     3241 attcctaagt agtcatcaaa tttcagctca gataaatttc gatcctaaac ttaattttaa
     3301 aaattccaat aataattcta ctgaattaaa aaagaaagat catgaaaccg caaagaaata
     3361 tcatcagaaa ttaacaggtg agtttttcgg tgcatcaatt gattttcaaa tcggttatgg
     3421 tactacctct ccaaatgtaa ctgaaaaaaa caatagtaaa gatgcttcta ctgaaagtca
     3481 gggtggattt actttcggag ctttggtaaa tcttaatctg tttggacttt tagatctgac
     3541 taccggtatt gatctgatta ataaaaaata tgaatacgga ataccttatg tgagtacaaa
     3601 tcctacagaa ggcgtagatt cagttgtaaa ttctgtaaag aatcaatatc tgaatattcc
     3661 attaaatata aattatggtg gtatggtttc tgaaaaagta ggactaagtt tcagcggagg
     3721 tccgtatttc gggtttcttt taaatccgga aagtgctgta agtggtttta aagattttga
     3781 ttttggattg aatggtataa ttacaggaaa ttatatgttg aatccgttta cgagcataat
     3841 acttggtact aaacttcagt acggaggact taataattta ttgagttcag gtaatataga
     3901 aagtgtttct ttaactaact gggatgtatt taccggtctc aggtttggat tctaaaaaat
     3961 atttttagta taaatttgaa ataaattaaa agggagtgat gctccctttt tttataaaat
     4021 taaaaaatga aaagttataa gtttgtaaat atactcttca tattattctc tgtagtattt
     4081 acattttcat catgcggaaa agaatcggat aattccaatg agcagtttgg atcagataat
     4141 tccaaaggaa atgaatctgt agcaaataaa gttattgatg attctgatat ttctgacaaa
     4201 tcaaatttgg cagatggtat atcagatctg aaatttggaa aaggtcttgt tccttctttt
     4261 ctgaaagatg aaaatcaaat tatagaagca gtgagctgga atgacaagct cggtgaaaac
     4321 ttgattgcaa ttactcttac agatgaaaga attataaaag gggatgagag aataaaagaa
     4381 ttatttatat atcattacat tgttaaaaat acttcttcag aagtcctgtg gaaaagttat
     4441 gattatgtga aagactgtcc gtttgatatt acacttgaat ttattgacag ctctctgact
     4501 gtgactgatc ttgacggaga tgacattgca gaaacttcat ttatgtataa actctcctgt
     4561 cgaagcgatg tgagtccgga tgatctgaaa ctcattatgc acgaaggtca ggataaatat
     4621 gcgataagag gggagatgat ccttaaaata aatgatgaag atccttcacc tggttctatg
     4681 attattgatg attcatttaa caagactaaa ggaattttta aagattttgc aatagaacaa
     4741 tggaataaat tcaataaagt tttcattggt gaaaaatatg aaaagtaaaa ttattttcag
     4801 taagcttttt atttttgttg cactggcttc tatgataatt gtcacgggct gttcaggatc
     4861 aaaagataca agtgatgaag aaacagaata tgcagaacat aatgaacata cagaaaaaga
     4921 ttctgaagta aatgtaaaat cagaagaatc cggatcatat attttaaaca tcgctgaggc
     4981 tgaacaaaaa aatgaaatgt ataaaataga agtggattct acttaccttt actggctgga
     5041 taattattta ctgatattaa atggttcgac taagtgtaat atatttgcat taaatgtttt
     5101 aaaaaaagcg ggctataaaa cgcctgaggt taatgcactt tcaagagatc tgtttgatac
     5161 actgaatttt aatgatatac ttccgatagt tggagtgaat gatgtttcag atgcaatgcc
     5221 aggagatctg gttatatgga acggacatgt tatattattt gaatctgaaa ttgaactgaa
     5281 tggtcaaagt tatgcaatgg catactgggc ggggacaaaa aatccggaca acggggataa
     5341 tattttgaac aatgtgtgct acggaaaata caaactaaat ggtaattacg tagtcaggag
     5401 acctttgaaa aaactttatc ctgactgaca attacagagc tttcagaatc ttgctgagaa
     5461 aatccacctg aatttttaag tccttcagag ttttgttatt tataattaca aaatcagaca
     5521 tttccatttt ctttctttca tcgatctgaa atttcataag ttccgtaatt gattttaaag
     5581 tagattcatc ccgaattttc agtctgtcca ctctttcttt cttttttgaa taaaccatta
     5641 ttatataatc catagattca tcaaatccac tttcaaacaa caaagcagat tccactatta
     5701 caatgtcata attgtttttc ttgaggaatc cttttatttc ttttattact acagggtgga
     5761 cgatcttatt gattgctgaa taattctttt tgcttgcaaa gatcttatcc tttaatttgg
     5821 gaagattgat cttaccttta taattaacta tatcttttcc aaacacttta actatttctg
     5881 taactaattt tttattagtt acataaagat tttttgcaat cgtatcagaa tataaaacct
     5941 tgaaacctct tttagcaagt aatttacata cttcagtttt tccggatcca acaccgcctg
     6001 ttacaccgat cattaatttc ttttttctaa tcattacgaa actttttttc ttttaaagta
     6061 aataaattat ttcgcatatg aaatagacct tctctctcta attacattaa cttttatttg
     6121 gcccgggtat tccatttctt cctgaatttt atgtgcaata tctgctgcaa gttcatcctg
     6181 aagagtatca tcgaccttat cctgttctac gatcaccctt acttctcttc ctgcctgaat
     6241 tgcataagtt tttactacac cttcaaatga ctttgcgata ttttctaatt tttcaagtct
     6301 ctttgaataa gcttcaatag attcacgtct tgcacctggt cttgcaccgc tgatagaatc
     6361 agctgcctgc actaagatcg atatcggatg ttccattggc atttcatcat gatgagctcc
     6421 aatcgcatta caaatgatcg gatgttcttt acatcttttt gcaacttcat atccgagaat
     6481 agcatgtggt ccttcaatat ttctgtcaat ggtttttccc atatcatgca aaagtcctgc
     6541 ccgctttgcc atttgtgcat caagtccaag ctcagctgcc attatacttg cgagatgtgc
     6601 tacttccata gaatgatgaa gtacattctg tccgtaactt gatctgtatt tcatccttcc
     6661 tattaatgtg attatatctc tgtgaactcc ctgaattccc atttcgataa gcgtattttc
     6721 tccgactctg atcagctctt ctgtcagctc tttctgtact ttttctacta tctcttctat
     6781 tctcgccgga tggatcctgc cgtctgctat aagtctttcc attgaaactc tcgcgacttc
     6841 tcttctgaaa ggatcgaatc cggaaataat tattgcttca ggagtatcat caacaataat
     6901 atccacacct gtaacagctt caaaagctct gatatttctt ccttcttttc caatgatcct
     6961 gccttttaat tcatctgaat ttatctgtaa aacactaact gtagtttcca cagaatgatc
     7021 agaagcagtt cgctgaattg attgaattat aatatttttt gaaattctgt ttgattctaa
     7081 cttcgtttct tctctaatct cctgaatttt ttgagcggct tcaatttttg catcattgat
     7141 aagtttttca aataaaactt ttcttgcttc ttcttcagaa aatcctgaga tccgctgaag
     7201 tttatttctt tgctcttcaa gtatttcaga attcattaaa attaatttct ctgactcaga
     7261 gatcttattt tcagcttctt tggttttgga atcacttaat tcgacagatt tatttagttt
     7321 gatttctaac ttggaaagtt cagatttaac agtttcgaga ttatcttttt caatcttaat
     7381 tcttttctca tcgactacca gatctttttc tcgatctttt acctcttcct catgttgttg
     7441 tttctttttt agaaattcat ctttgacttc aagaagcttt tctttcttta atgtctgaga
     7501 tgctttttca gcatctgaaa gaatttcttt cgaacgtttt tcagcaattt ctatcttgtc
     7561 ttttgatttt gactctgcat ctgataatat ttttttcgct acgttatctg cgccttcaat
     7621 ctttgtttta tttgatcttg agtttatata ccagccgagg aaaaaggtta acaggcataa
     7681 agctacaaga attggtaata atatgtataa ttctagatcc ataatttatt ttatatcctc
     7741 cataataact gtgtgttttt ttaattaaat atatttttat agtttgtatc attttaaatt
     7801 tgatataatt ttttattcta aaatgaataa aaaaaaccgc tgcagactgg ataatagcgc
     7861 aaagggtata aaaaaacctt tcctatcata cggtgggtgg tgccagaatg tcttttgctt
     7921 ccactgatcg ctattgattc ttttcagaat cagcgaatcc gctaatgcgg ctgaggcctg
     7981 taattgacat tttccgagcg tgcctcccga atagttaaac ctgttggttt tttataatgt
     8041 ttgactaata agtctgagcg gttaattaaa acctaaattt tatataaaga acggtatttt
     8101 caatttcaca aattatcatc gatcatatct gaaattgaat ttgattttga gatcaattca
     8161 tccagaagat ctgaataatc tttttcaagg tcgagcttaa tgtccttttc cttgaaatat
     8221 tcctcggcta tgtttaatgc tgaaactact gctatagttt cctccgattg atttggtgat
     8281 tcactgttaa gacgattcat gatcccgtcc acgaaatcag aaattctttc taccttctca
     8341 gtactatcac cctgcagatt atattcatta ttgaatatct taactcgaat ttctttttgg
     8401 ctcatatctc aataatgatt aataattagt gaacttgaaa attgtaaagt caaacatttt
     8461 agttgtctct gtattctcca tcgttatcat attgatctaa atgcgtgtct attcttgtta
     8521 taagatcttg tattcttgtt tttgcataat ctttatcttt cattggaatt ttattatcac
     8581 ctgaattcaa tactacattt ttcagatcag aattttcttt gtctctgttt attatttcag
     8641 tattgagctt tgtatgttgt aattttaatt ctgaaatttt taagttcagg ttctttaaat
     8701 tttcctttag taaattattc tcatttttca ggtctttgta tttagaaatt aatgatttga
     8761 tctttgaaaa aaggtcatca agagatactc tcaacacatt tttctgatct ttaaaaaaat
     8821 ccttagtggt ttcctgattg ttaagattca ttttttttaa ttaa
//
LOCUS       DAORVQ010000140         8836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4405_length_8836_cov_5.867783, whole genome shotgun sequence.
ACCESSION   DAORVQ010000140 DAORVQ010000000
VERSION     DAORVQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4405_length_8836_cov_5.867783"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1405
                     /locus_tag="PLX80_10690"
     CDS             86..1405
                     /locus_tag="PLX80_10690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQY21297.1"
                     /translation="MKIAIVGTGYVGLVTGTCLADTGNNVICVDIDTEKVDKLKNNIL
                     PIYEPDLDTIFERNVEEGRLEFTTDLKYAADNSDIIFLALPTPPGEDGSADLSYILKV
                     ADDLGKIITSYKIIINKSTVPVGTADKVREAISKNTDVEFDIVSNPEFLKEGLAVDDF
                     MKPDRIVIGTKSEKARKVLEELFTPFVRSGNPIIFMDERSSELTKYASNSYLATRITF
                     MNEIANICERIGADVDLVRIGMGSDSRIGKRFLFPGIGYGGSCFPKDVSALHKSAKGM
                     DYNFDILDSVMKVNETQKTIIVEKILKYYNNDIKGKKFALWGLAFKPDTDDIREAPAL
                     YIIEKLLNAGAVISAYDPEAMENVKKEFGDRIEFVSNNYDALKDSDALIIATEWTVFR
                     TPDFKKMADNLKEKVIFDGRNLYDLAQMEKLGFYYASIGRRIIDSKK"
     gene            1473..2639
                     /locus_tag="PLX80_10695"
     CDS             1473..2639
                     /locus_tag="PLX80_10695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY21298.1"
                     /translation="MKVPLLDLKAQYRTIKEDIDKALISVAESQGCILGPEVEKLEKD
                     LAAYLDCKYAIGVSSGTDALLVALMAIDIKPGDEVIIPTYSFFATAGVVSRLNATPVF
                     IDSDPVTFNIDPTKLEALINEKTKAIVPVHLYGQCADMDPIMEIANKHGLMVIEDGAQ
                     AIGSQYKDGRKAGSIGQIGCFSFYPSKNLGCFGDGGLVTTNDETLYERLKIMRVHGGK
                     PKYYHKVIGGNFRLDAIQAAVLNVKFPHLDSWTAKRQENAELYTKFFKDHGLAEVEGK
                     LEFDERNKVLLPEPVYKKNDLKYYHIYNQYVIRVENRDELRKYMTENEIGSEIYYPVP
                     FHLQECFADLGYEKGDFPVSESCAADTIALPIYPELTNEQIEFTVKTIGDFIKK"
     gene            2682..3725
                     /gene="gmd"
                     /locus_tag="PLX80_10700"
     CDS             2682..3725
                     /gene="gmd"
                     /locus_tag="PLX80_10700"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY21299.1"
                     /translation="MKKALISGITGQDGSYLTELLLEKGYEVHGIIRRSSSFNTKRID
                     HLYAQKDLLDKRLFLHFGDLVDTSSLNRLLEKIEPDEIYNLAAQSHVKVSFEVPDYTA
                     QVDALGTLRFLDAIRETDLRQVKFYQASTSELYGKVQEVPQTESTPFYPRSPYGVAKI
                     YGYWIVVNYREAYNIFACNGILFNHESPRRGETFVTRKVTRAVARIKHGLQKTLSMGN
                     IDSKRDWGYAPEYCEAMWRMLQTDTPEDFVIATGETHSVREFIEESFRHIGKDIEWKG
                     KDENETGVDAKTGDLLIEIDKNYFRPTEVDLLIGDPSKAKKILGWEPKVTFKELVKIM
                     VEADSIMVEKKGY"
     gene            3729..4247
                     /gene="rfbC"
                     /locus_tag="PLX80_10705"
     CDS             3729..4247
                     /gene="rfbC"
                     /locus_tag="PLX80_10705"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQY21300.1"
                     /translation="MPITVSDTELKGVFYIEPTFFQDHRGKYIETYNIDDYEKNGITV
                     KFVRDDISTSVKNVLRGIHYDNKTWKLIQCMYGEIFFVVVNMNENSDEYLKWQSFILN
                     SETRHQVLVPPGFGNGHLVLSNECIFHYKMSEYYDPDNEKGIKWDDPKLNINWPVKDP
                     ILSEKDSATKYL"
     gene            4278..5465
                     /locus_tag="PLX80_10710"
     CDS             4278..5465
                     /locus_tag="PLX80_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQY21301.1"
                     /translation="MKIRENFLSLAVPDISEEEKKEVLEALDSGWISVGPKVKEFENK
                     FAEYHNVKHAIAMSSCTTALFIAAKVLGITKGDYVIVPTITWQSTANIVEQLNATPLF
                     CDVEKDTLNISINDINDHIKKYGNKIKAIIPVHHSGLPADIDAITEISDKHGIPVIYD
                     AAHAVFSSYKDKMIGQYGTMSTFSFYATKNLTTGDGGMITTNDDNLAELCRLWSYHGL
                     PKDSWKRYSAENASPHVQSIVPGYKFNLTDIQAALGLAQMKRKDDLLSKRNFLVEKYN
                     ELFKDTDWIERPVFKTEYGKWGNHVYVIKILDDDINRDKLMQELRKFNIGTNLHFYPV
                     HKNIFYEKKYPDEKLPVAEWLMDRILTLPLCTKYSVEDLEYVKASLDHIYENKLANKT
                     VFV"
     gene            5488..6435
                     /locus_tag="PLX80_10715"
     CDS             5488..6435
                     /locus_tag="PLX80_10715"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY21302.1"
                     /translation="MSKVLITGGAGNIGSFIADQCIDLGHEVIIVDNFYNGKHRNISE
                     HVKSGKAELETIDIGSYEMVKYAFDKHKPEYVSHQASLMILDSNKFPHKAIDTNITGT
                     FNIIQACHDFGVKKLTYGSSASVYGDPRFIPVTEEHPFDNRTLYGATKIAKEALMNSW
                     AYSLGVPFVGFRYFNIYSERQGLGAFYTQVFQKWIHAIYNDEEIIMYGDGEQTLDLVH
                     SSDAARANVLALFNNDVKNEHFNVGTGIETSLKEILALIEKMLGKKAKVKHIDSDPHL
                     VKRRCSSIEKIKSRLGFEPEVTVEEGTGKYISYLLEGKI"
     gene            6435..7445
                     /locus_tag="PLX80_10720"
     CDS             6435..7445
                     /locus_tag="PLX80_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21303.1"
                     /translation="MLYDKKHIYLRSPLRISFVGGGTDLESYYLNEGAGHVVSSTIDL
                     YNYVSIKDMFDSNVRVHHAEIETESITSRIKHTYSRTALEHFGMFKGAEVILTSDVMT
                     TGSGLGASSAMMSALISGCSAIRDNKIKTNTELAELTYKLESDAGTIGGKQDQYATAF
                     GGFNSITFDKNNVKVKTVKISKDKLKHLEDRVFLVFTNMAREISSIQADLGKNIFDES
                     KRKYFSELKDLSYEFLNTLESKNGNLNEVGKLLHESWKLKKSTNNNTSNQFIDQLYDM
                     LRKKGVTGGKILGAGGGGFLLAYTPDPKLKAKLKYDLYPNFIALDIKFSPKGTETLWK
                     SF"
     gene            7430..8107
                     /locus_tag="PLX80_10725"
     CDS             7430..8107
                     /locus_tag="PLX80_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HQY21304.1"
                     /translation="MEEFLGVLFCGGRGTRLGEITKYISKSFVPVYDRPVFKFGLELL
                     TNSSNISDIIILTNEMNHDALSREGYKTLLQDDSKVNDMFTGWEFIKEKTGTKANGVL
                     VPSDNIINADIDFMIKTFEEKKCDLLFSLLKVNNKQKLSEMGSFNFNDRKFQYKNPSP
                     EPNRDFGVLAPYIIRNNFQFNSDNIIFETDNSEILFYDGYWTDIGDVQSLAEANAHFQ
                     KVRKKNK"
     gene            8114..>8836
                     /locus_tag="PLX80_10730"
     CDS             8114..>8836
                     /locus_tag="PLX80_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY21305.1"
                     /translation="MKILINFASKLGVDTVSRLIGFLTLPLITRALGPDGYGQFTYLF
                     IILSYFGFFIDFGYLNYGTNKLCEKDGNENVISKIISLQIITAFFSYIILVVVAYFFL
                     DTEKYILLLIFSLTFITQIFSIKYYYLAKNKLYYNSISEFAGQAVYAALVFTVFVAKP
                     EVITLIILTIFQTGVTALFLIIPFIKKQKLKLDFSLKNNLKTLKEAYKLGLASKAEAV
                     TATFIILCLGFFLNEESVGIYNA"
BASE COUNT         3052 a         1460 c         1748 g         2576 t
ORIGIN      
        1 aaatataaaa cagcaatacc aattatttaa taatgtttat taaaatttta aaattataaa
       61 ttaacagaaa ggaaaaaatc gtttaatgaa aattgcaatt gtaggtaccg gatatgtagg
      121 acttgtaaca ggtacgtgtc ttgcagatac agggaacaat gttatttgcg ttgacataga
      181 tacggaaaaa gtagataagt taaaaaacaa tatcctgccg atatatgagc cggatcttga
      241 tactattttt gaaaggaatg tcgaagaggg cagactcgaa tttacgaccg atctgaaata
      301 tgctgccgat aactcagata taatatttct cgcgctgccg actcctccgg gtgaagacgg
      361 ctctgctgat ctttcatata ttttaaaagt cgcagatgat ctcggtaaga tcattacatc
      421 ttataagatc attattaata aaagcaccgt ccccgttggt actgcagaca aagtaagaga
      481 agctatttca aaaaatactg acgtcgaatt tgacattgta tcaaatccgg aattcctgaa
      541 agaaggactt gcagtggatg acttcatgaa gccggacaga attgtgatag gtacgaaatc
      601 cgagaaggca agaaaagttc ttgaagaatt attcacgccg tttgtaagat cgggaaatcc
      661 aattattttc atggatgagc gttcatccga acttacaaaa tacgcatcga attcttatct
      721 tgctacaaga attactttca tgaatgagat cgcaaacatc tgcgaaagga tcggcgctga
      781 cgttgacctt gtgaggattg gaatgggaag cgactcgcga atcggaaaga ggtttttatt
      841 tccgggtatc ggatacggcg gaagctgttt cccgaaagac gtaagtgcat tgcataaatc
      901 cgccaaagga atggattaca attttgatat tttagattct gtaatgaagg tgaatgaaac
      961 tcagaaaaca attatagtag agaagatctt aaaatattat aacaatgata ttaaaggtaa
     1021 gaagtttgct ttgtggggac ttgcttttaa gcctgataca gatgacatca gagaagcacc
     1081 ggctctttat attattgaaa aacttctgaa tgccggagca gtaatttctg catacgatcc
     1141 tgaagctatg gaaaatgtga agaaggagtt tggagacaga atagaatttg tcagtaataa
     1201 ttatgatgca ctcaaagatt cagatgcact tataattgct accgaatgga ctgtcttcag
     1261 aacacctgac tttaaaaaaa tggctgacaa tctgaaagag aaagttattt ttgacggaag
     1321 gaatctttac gatctcgcac agatggaaaa gcttggattt tattatgcaa gtatcggcag
     1381 aaggatcatt gattcaaaaa agtaatatga atgtttattt ttattaatag agttataaaa
     1441 attttcaaaa atcaaaaacg aaaataaaat atatgaaagt cccattatta gatcttaagg
     1501 cgcaatacag aacaataaaa gaagacattg acaaagctct gatcagcgtc gctgaatcac
     1561 agggctgcat actcggacct gaagtcgaaa agctcgaaaa agatctggca gcttatcttg
     1621 actgcaaata tgcgatcggc gtttcctcag gtacggatgc attactcgtt gcgcttatgg
     1681 caattgacat aaaacccggt gatgaagtta tcattccgac atattcattt tttgcaactg
     1741 caggtgtagt cagcagacta aatgcaactc cggttttcat tgactccgat cctgttacat
     1801 ttaatatcga tccaactaaa ctcgaagcac tcattaatga aaagacaaaa gcaattgtac
     1861 ctgttcatct ttacgggcag tgtgcagaca tggatcccat tatggagatc gcaaataagc
     1921 atggacttat ggtcatcgaa gacggagcgc aggcaatagg cagtcagtat aaagacggca
     1981 gaaaagccgg cagcatcggt cagatcggtt gcttttcatt ttatccgagt aaaaatttag
     2041 gatgcttcgg agacggcgga ctggtcacta ccaatgacga gacactttat gaaagactga
     2101 agatcatgag agttcacggc ggtaaaccaa aatattatca caaagtaatc ggcggcaact
     2161 tcagacttga tgcaattcag gcagcagtat taaatgtaaa atttcctcat cttgattcat
     2221 ggacagcaaa gcgtcaggag aatgctgagc tttatactaa attttttaaa gatcacggac
     2281 ttgcagaagt cgaaggcaaa ctcgaatttg atgaaagaaa taaagttctt ctcccagaac
     2341 ctgtttacaa aaaaaatgat ctgaaatatt atcacattta taatcagtat gttatcagag
     2401 ttgaaaacag agatgagctt agaaagtata tgacagaaaa tgaaattggt tcagagattt
     2461 actatccggt acctttccat ttacaggaat gttttgcaga tctcggatat gagaaaggtg
     2521 atttccctgt gtctgaaagc tgtgctgcag atacaattgc attaccgata tatcctgagc
     2581 tgacaaatga gcagatcgaa tttactgtaa agacaattgg tgattttata aaaaagtaat
     2641 tttatttttc taaaactgat atttttaaat aaacacaatt aatgaaaaaa gctttaatat
     2701 ccggtatcac cggacaggac ggaagttatt taacagaatt attactggaa aaaggatatg
     2761 aggtacatgg aataataaga agaagcagtt catttaacac aaaaagaatt gaccatttgt
     2821 atgcgcagaa agatctttta gacaaaagat tatttttaca ttttggtgat cttgtggaca
     2881 caagcagtct gaacagatta ctcgaaaaaa ttgagcccga tgaaatttat aatctggcag
     2941 ctcagagtca tgtgaaagtt tcttttgaag ttcctgacta tactgctcag gtagatgcac
     3001 ttggtacgct gagatttctt gatgctatca gagagaccga tctgagacag gtgaaatttt
     3061 atcaggcatc tacaagtgaa ctttatggaa aggtgcagga agttccgcaa actgaatcaa
     3121 ctccatttta tccaagaagt ccttacggcg ttgcaaagat ctacggttac tggatagttg
     3181 taaattacag agaagcttat aatatttttg catgcaacgg aattttgttt aatcatgaat
     3241 ctccaagaag aggtgagact tttgtcacaa gaaaggttac acgagctgtt gcaagaatta
     3301 aacacggtct tcagaaaact ttgtcaatgg gaaatatcga ttcgaaaaga gactggggat
     3361 acgcacctga atactgtgaa gctatgtgga gaatgctgca gacagacact cctgaagatt
     3421 ttgtaatcgc cacaggtgaa acacattctg tccgtgaatt tattgaagaa tctttcagac
     3481 atatcggaaa ggatattgaa tggaaaggta aagatgaaaa tgaaacagga gtcgatgcaa
     3541 agacaggaga cttacttatc gagatcgata aaaattattt cagacctact gaagtagatc
     3601 tgctcattgg agatccgtcg aaagcaaaga agatccttgg atgggaacca aaagttacat
     3661 ttaaagaact tgtaaagatc atggtcgaag ctgattccat aatggtagaa aagaaaggtt
     3721 attaataatt gccgatcact gtctctgata ctgagctgaa aggcgtcttt tatattgaac
     3781 caacattttt tcaggatcac agagggaaat atattgagac atataatatt gatgattatg
     3841 aaaagaacgg gatcactgta aagtttgtac gagacgacat ttccacatct gtaaagaatg
     3901 tactgagagg aattcattat gataataaga catggaaact gattcagtgc atgtatggtg
     3961 aaattttttt tgtagttgta aacatgaatg aaaattctga tgaatattta aaatggcagt
     4021 catttatttt aaacagtgag acgcgtcatc aggtacttgt accgccgggt tttgggaatg
     4081 gacatctggt attgagcaat gaatgtatct ttcattataa gatgtctgaa tattatgatc
     4141 ctgataatga gaaaggaatt aaatgggacg atccgaaact gaatatcaac tggccggtta
     4201 aggatccgat actttcagaa aaagattcag caacaaaata tttatagaat tatatcaact
     4261 aactaaattt ttcggtaatg aagatcagag aaaatttttt gtcactggca gttccggata
     4321 tttccgaaga agaaaaaaaa gaagtactcg aagcacttga ttcgggatgg atcagcgtcg
     4381 gtccgaaagt taaagagttt gaaaataaat tcgctgagta tcataatgta aaacatgcaa
     4441 tagcaatgag ctcatgtact actgcacttt ttatagctgc aaaagttctc gggattacca
     4501 aaggcgatta tgtcatagtt ccaactatca catggcagtc aacagcaaac atagtcgaac
     4561 agctcaacgc gactccccta ttctgtgatg tcgaaaaaga tactttaaac attagtatta
     4621 atgacatcaa tgatcacata aaaaaatacg gtaataaaat aaaagcaatt atacctgtcc
     4681 atcattcggg acttccggca gacattgatg ctattacaga aatttctgac aagcacggaa
     4741 tacctgttat ctatgatgcg gcacatgcag tgttttccag ttacaaagat aagatgatcg
     4801 gacaatacgg taccatgtca acattcagct tttatgcaac caagaatctt actacaggcg
     4861 acggaggaat gatcactaca aatgatgaca acttagccga actatgcaga ctttggtctt
     4921 atcatgggct tccaaaagat tcatggaaaa gatattcggc agagaatgca agtccgcacg
     4981 tgcagagtat cgtccccgga tataaattca atctcacgga tattcaggca gctcttggtc
     5041 tcgcacagat gaaaagaaaa gatgatctgc ttagcaagag aaatttttta gtcgaaaaat
     5101 ataatgaact ctttaaagat actgactgga tagaaagacc ggtatttaaa actgaatatg
     5161 gtaaatgggg aaatcatgtg tatgtaataa aaattctgga tgatgacatt aacagagata
     5221 agctgatgca ggagctgagg aaatttaata ttggaaccaa tctgcatttt tatccggtgc
     5281 ataaaaatat tttttatgaa aaaaagtatc ctgatgaaaa acttccggta gctgagtggc
     5341 tcatggacag aatacttact ctgcctctgt gtaccaaata ttcagtcgaa gatctggagt
     5401 atgtaaaagc ttcacttgat catatttatg aaaataaact tgccaacaaa actgtttttg
     5461 tttaaaattt aattaataat ttttttcatg tcaaaagttc taatcacagg cggcgcagga
     5521 aacatcggat cattcattgc tgatcagtgc attgatctcg gtcatgaagt tattatagta
     5581 gataattttt ataacggaaa gcaccgcaac atttcagagc atgtcaaaag cggtaaggct
     5641 gagcttgaaa ctattgacat cggaagttat gaaatggtga agtatgcttt tgataaacat
     5701 aaacctgaat acgtttcgca tcaggcttca cttatgatcc ttgattcaaa taaatttcct
     5761 cataaagcaa ttgacacaaa tatcacaggt acatttaata ttattcaggc atgtcacgat
     5821 tttggagtaa agaaacttac ttacggttca tcagcgtctg tctatggtga cccgcgtttc
     5881 attcccgtta cagaagagca tccttttgac aacaggactt tatatggagc aacgaaaatt
     5941 gctaaggaag ctctgatgaa ttcatgggca tactctctcg gagttccatt tgtcggattc
     6001 agatatttca atatttatag cgaacgtcaa gggctcggag ctttttatac acaggttttt
     6061 cagaaatgga tacatgcaat ttataatgat gaagaaataa taatgtacgg agacggcgag
     6121 caaacgcttg acctcgttca ctcatctgat gctgctcgtg caaatgtact tgctcttttt
     6181 aataatgatg taaagaatga acatttcaat gtaggtacag gaatagaaac ttcacttaag
     6241 gaaatactag cactgattga aaaaatgctt ggtaaaaaag caaaagtgaa acatatcgat
     6301 tcggatcctc atctggtgaa aagaagatgt tcatccattg aaaaaattaa atcccggctt
     6361 ggctttgagc cggaagttac tgtcgaagaa ggcacaggaa aatatattag ttatttattg
     6421 gagggaaaga tctgatgctg tatgacaaaa aacatattta tctgagatca cctttacgaa
     6481 taagttttgt cggcggcggc acagatttag aatcttatta tttaaatgaa ggtgcgggac
     6541 atgttgtttc ttctactata gatctttata attatgtctc tataaaagat atgtttgatt
     6601 cgaatgtaag agttcatcac gcagagatcg agacagagtc tatcacgagc aggatcaagc
     6661 atacttattc acgcacagct ctcgaacatt tcggaatgtt caaaggagct gaagttattc
     6721 ttacatctga tgtaatgacc acaggcagcg ggcttggagc atcttcagca atgatgagtg
     6781 cactgatatc cggctgcagc gcgatcagag ataataaaat taaaactaac acagagcttg
     6841 ccgaacttac ttataagtta gaatctgatg caggaacaat tggcggtaaa caggatcagt
     6901 atgcaactgc attcggcgga ttcaattcaa ttacctttga taaaaataat gtcaaagtaa
     6961 aaacagtgaa aatatcaaaa gataaactta agcatctcga ggacagagtg ttccttgtat
     7021 ttaccaatat ggcaagagag atcagttcta ttcaggcaga tctcggaaag aatatttttg
     7081 atgaatcaaa aagaaaatat ttttcagaat taaaagatct ttcatatgaa ttccttaata
     7141 ctctcgaatc aaagaacgga aatctcaatg aagtcggcaa acttcttcat gaaagctgga
     7201 agttaaaaaa atccactaat aacaatacat caaatcaatt cattgatcag ctttacgaca
     7261 tgctgagaaa aaaaggagta accggcggga agatcctcgg tgccggcggt ggaggatttt
     7321 tgcttgctta tacacctgac ccgaaattaa aagctaagct caagtatgat ctgtatccaa
     7381 acttcattgc actcgacata aaattcagtc cgaaaggaac cgaaacatta tggaagagtt
     7441 tttaggagtc cttttttgcg gaggcagagg gacacgtctt ggagagatca caaaatacat
     7501 ttcgaaatct tttgtaccgg tttacgacag acccgttttc aaattcggtc ttgaattact
     7561 tacaaactct tctaatatca gcgatataat aattctaaca aacgaaatga atcatgacgc
     7621 tctcagccgt gaaggctaca aaactttact tcaggatgac agcaaggtta atgacatgtt
     7681 cacaggctgg gaatttataa aggaaaaaac cggcacaaaa gcaaacggtg tgcttgtacc
     7741 gagtgataat attattaatg ctgatataga ttttatgata aaaacttttg aagaaaaaaa
     7801 atgtgatctt ttattttcac tgcttaaggt caataacaaa caaaagcttt ctgagatggg
     7861 aagttttaat tttaatgaca gaaaattcca atacaaaaat cctagccctg aacctaatag
     7921 ggacttcggg gtattagctc cctatataat ccgaaataat tttcaattta attccgataa
     7981 cattattttt gaaactgata attcagaaat acttttttat gatggatact ggacagatat
     8041 aggtgatgtg caatcacttg ctgaagctaa tgcacatttt caaaaagtcc gtaagaaaaa
     8101 taaataacag tttttgaaga tacttataaa tttcgcttcc aagctgggag tcgatactgt
     8161 ttcaagactt atcggatttc tgacactgcc gctgatcacg agagctctcg gtcctgatgg
     8221 ctatggacag ttcacatatc tttttatcat tctttcttat tttggttttt ttattgactt
     8281 cggatatctt aactacggaa ctaataaact ctgtgaaaaa gacggcaacg aaaatgtcat
     8341 cagtaaaata atttccttac agataatcac tgcatttttc tcttatataa ttctagttgt
     8401 agtagcttat ttttttctcg atacagaaaa gtatattttg cttctgattt tttccctgac
     8461 atttataacc cagatctttt ctatcaaata ttattatctc gcaaaaaata aattatacta
     8521 caattctatc tctgaattcg ccggtcaggc tgtctatgct gcgttggtat ttacagtttt
     8581 tgttgcaaag cctgaagtaa ttactcttat cattctgact atttttcaga caggcgtgac
     8641 agcattattt ttaataattc cttttattaa aaagcaaaaa ctgaaacttg attttagttt
     8701 aaagaataat ttaaagacgt tgaaagaagc atataaactg ggacttgctt caaaagccga
     8761 agcagtgacc gctactttta tcattttatg tctgggattt tttcttaatg aagaatcagt
     8821 aggtatctat aatgct
//
LOCUS       DAORVQ010000141         8799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4435_length_8799_cov_5.762923, whole genome shotgun sequence.
ACCESSION   DAORVQ010000141 DAORVQ010000000
VERSION     DAORVQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4435_length_8799_cov_5.762923"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..242)
                     /locus_tag="PLX80_10735"
     CDS             complement(114..242)
                     /locus_tag="PLX80_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21306.1"
                     /translation="MTKRTDDKSEGGQKERSFTTLRKTKYFDSNSDLNIPMALGNV"
     gene            813..1853
                     /locus_tag="PLX80_10740"
     CDS             813..1853
                     /locus_tag="PLX80_10740"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HQY21307.1"
                     /translation="MSIKSNIAAVFIIFMSILFIQGCDDKKNEGSNSKGSDTVNADLI
                     TKDDKGTKVSLLLKPKVGDVLKYKMNAKTTSKENSPLTEGKELTSIQDINYYYSEEVT
                     DITSAGIVSYKITFDSITIISNLASGDSSVSLTYNSNIKDSIYSKPDFLQYNAIMAED
                     FYARVGQNGEISDIYGLEKVYEKMFKALGDTLSNEQKESLKDSFGRDAIKAVLQQQFQ
                     MFPESAVYLDSVWTRSYETQMLIFPVRNSLSYKLNEVKEENNQTILTIDADLAVDFIE
                     KEIKDKQMSYKVENSETGGKGLIVYNLTKGCVVKKETSTNLKLDMKISAGSQSVNTTQ
                     DVTTELFVNLIQ"
     gene            1872..2516
                     /gene="upp"
                     /locus_tag="PLX80_10745"
     CDS             1872..2516
                     /gene="upp"
                     /locus_tag="PLX80_10745"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQY21308.1"
                     /translation="MKKLKIIDHPLVQHYLSIMRDKKTSQVDFKESLDKISYILATET
                     FGSLSLENKAVFTPFKKIEGKKVSDTVILLPILRAGLGLTKGFTDLYPEILTSHIGIY
                     RDEESLKPVQYYFRFPKIEDKKEVKVIILDPMIATGGSVIFTIQYLLNMGIRKIKVVS
                     LLCAPEGIEAIEKRFGKDEKKHLEIITCSLDEKLNEKGYIVPGLGDAGDRIFGT"
     gene            2703..4922
                     /locus_tag="PLX80_10750"
     CDS             2703..4922
                     /locus_tag="PLX80_10750"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQY21309.1"
                     /translation="MLNNLNKKKLDDLLYHCRKNLPAGLSNEKLITHAFKFAYEAHKN
                     AKRMSGEPFFSHPYEVAIILAQEIPLDAVSIAAALLHDVVEDTPFTLNDIEAEFGKEI
                     ADIVDGATKIEGMFENYEMKQVESYKKMLLSMTSDIRVILIKFADRLHNLRTLEFLSN
                     ERQVRLAQETLEIFAPLAHRFGLSSVKTELEDMSFKYLNRTAYDEIAKKLQSKRRERE
                     KTIKKFIEPIKQRLNEDGFKFEIYGRAKHLYSIYKKIISRNKSFEELFDLFAIRIILD
                     TKNKNDCFSVYGICSEIYIPVPERFKDYVSLPKQNSYQSIHTTLVSKEGKMFEVQIRT
                     REMDEVAEKGIAAHWKYKENNNIHDKKLEDWMKTIRESIENSSKDETSSEVMENFKLN
                     LYQDEIYCFTPKGDLKILPSGATAIDFAFDIHSQIGMKCIGAKVNGKIVSLDTHLQSG
                     DQIEILTSKNQHPKADWEKFAVTHKAKSDIRKFLNTEKRNFIQKGKELWEKKSKKSKV
                     KLSDEDLTKVVHKFKYKDTGNFFHAISIDESKINQVIDFISDKTKLITLDQKLTAPED
                     YKGTEEENFDKYVDEARTSANGIFIGNGTSQNIKGIKYEYAKCCNPIPGDEVIGFISK
                     EKGIRIHRKTCKNILNLFLKEPERIVQINWNEAESGEFTGGVKIIGEDRPGILNEITK
                     TISKNFNTNIKSVDIKTKSSMFEGTLILNIQNLKQLNQIIDKITQQEGVFSVTRFEL"
     gene            4951..5994
                     /locus_tag="PLX80_10755"
     CDS             4951..5994
                     /locus_tag="PLX80_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21310.1"
                     /translation="MTFLFSFKQTETAENKTASISKDDSLILPGEIHFENLKQLTFGG
                     QNAECYFSPDGTKFSFQSTNGDLNCDQIFTMDLEGNNKKLVSTGKGTNTCAYYLPGNT
                     KVIFSSTHEASDDCPPKPDFSKGYVWAIYDTYDIYSANEDGTDLKKLTNEKGYDAEAT
                     VSPKGDRIVFTSTRNGDIDIYSMNTDGSDVKQLTDTLGYDGGPFYSYDGSKIIYRRTT
                     FENEESVIDYKELISQGLIKPSQLDIWIMDADGSNKKQITKNSAANFAPFLFPDGEKL
                     LFCSNVGNEDKRNFDIFKINIDGTDLERITLYDEFDGFPIFSPDGKKLAFSSNRNGSA
                     KGETNVFICDWVD"
     gene            complement(6012..6425)
                     /locus_tag="PLX80_10760"
     CDS             complement(6012..6425)
                     /locus_tag="PLX80_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21311.1"
                     /translation="MYRIILIFVIFIMSCSSSEKIIHSDIEGIWINQLQSAGEIGSDR
                     KYYQFIFTKDSFNIEIGELNADTDLSYEPFRAKGLYKIRGDIINMKGVAKAEGFTKYN
                     VNFEEKFEYAFDENTIVLFPQGNIVSNRFYLIRRF"
     gene            complement(6429..6797)
                     /gene="acpS"
                     /locus_tag="PLX80_10765"
     CDS             complement(6429..6797)
                     /gene="acpS"
                     /locus_tag="PLX80_10765"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQY21312.1"
                     /translation="MIIGLGTDIIEVKRIKKIMDDYGDRFLKRILTENEIKYCMKFSK
                     PELHLAGRFASKEAYSKSIGTGVGKSFSWKDIEILNNERGKPYIVHLKESEFSVYKFE
                     ISISHTDEYACAVVSCEDGK"
     gene            complement(6807..7493)
                     /locus_tag="PLX80_10770"
     CDS             complement(6807..7493)
                     /locus_tag="PLX80_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02253 family HAD-type hydrolase"
                     /protein_id="HQY21313.1"
                     /translation="MIKAIIFDLDNTLVDFMLLKRAAVNAAIYAMIDAGLKLSHAETL
                     DMINKIYESEGIEYQQVFDNLLHEALGKVDYKIMSAGIVAYRKAREAALKPYPGVLPT
                     LIELIKMGLKLAVVSDAPSKEAWLRLSYINFQHLFDVVITYDETRERKPSPVPFNLAL
                     QELGLKANECLMIGDWAERDMVGAKAVGMKTVFARYGDTFDTVNPESDYDINSISELI
                     EIIKKENSDI"
     gene            complement(7521..7973)
                     /locus_tag="PLX80_10775"
     CDS             complement(7521..7973)
                     /locus_tag="PLX80_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQY21314.1"
                     /translation="MKKKIFQNKYDSDTEEFKLILNSHSQKITKPRLEILKILKSNHK
                     PLTISEISGKLIDDSIDLATVYRTMNLFEELNIVSLIDFKDEFKRYELIYDRHHHHHI
                     VCKNCKKVENVETCILDNIEKFLTKKGYTEISHSLEFFGICGECRVKV"
     gene            complement(7970..>8799)
                     /locus_tag="PLX80_10780"
     CDS             complement(7970..>8799)
                     /locus_tag="PLX80_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY21315.1"
                     /translation="GTMGRGIAICLALSEIDTIVYDIKDEIVNSSKSLTEKDLSKSVE
                     KKKLTEEKKSKALKRLSFTSEFDDLSECDFIIEAALENIDLKKDIFGRLDKICREDAI
                     LSTNTSSLSVTNISTAVKTRPERVIGMHFFNPAHIMKLVEIVKGDFTSDEVLKATVEL
                     TKKMNKVPVVAKDTPAFIVNRIARPFYGEALKILSEGTSDAETIDKLMKACGGFAMGP
                     FELMDLIGIDVNFSVTKSVYEAYFNDPKYRPSFIQKKMVDANLLGRKTGRGFYNYEK"
BASE COUNT         2931 a         1540 c         1481 g         2847 t
ORIGIN      
        1 cggctggaat cattttatct ttttattttc agcagtcttt gtttcgattt cagagttttt
       61 tgtttttttg cttcgttagt tcgtcctgta aataaattat acaggtcgat ttttcatacg
      121 ttccccaaag ccattggaat gtttaaatct gaattggaat cgaaatattt tgtctttctg
      181 agagtagtaa atgatctttc cttttgtcct ccttcacttt tgtcatccgt tctttttgtc
      241 atccgttctt ttttatcatc cgttcttttt tgccatccgt tcttctatgt catcctgagc
      301 gaagcgaaag atacatgtaa gttattaccg ctgcctttag ctggattctt cactgcgctc
      361 gcttcgctca cttgttcaga atgacaaagt gttttgtcat cctgagcgaa gcgaaggaac
      421 catgtaagtt gttaccgctg ccaattgcat ggattcttca ctgcgctcgc ttcgctcact
      481 tgttcagaat gacaaagagt ttttttcatc gttgttgctc ttagtgagcg cagcaagcgt
      541 aaccaacaac aaacggaaac tagtaggttg cagctgtgaa catcggattt gatatataaa
      601 aatattgtgt ttcgccgtta ttggtctgaa tgagtcaata gagtgtgatt aacaacaaac
      661 aaatattctg aaatagctta aacattttca tttcgatttt taatttaaat tctgttaaaa
      721 attctcgatc tccgactgaa ttatactttg aaactcttac ctataaatgg tatatttcac
      781 gatatttttt ataacctaac caatcatatt taatgtccat aaaatcaaac atagcagcag
      841 tttttattat attcatgtca atcttgttta ttcagggatg tgatgacaaa aaaaatgaag
      901 gcagtaacag taaaggttca gatactgtaa atgccgatct gatcacaaaa gatgataaag
      961 gtacaaaagt aagcctgcta ctgaaaccaa aagtcggtga tgtgctgaaa tataaaatga
     1021 atgccaaaac cacttcaaaa gaaaacagcc ctctcacaga aggcaaagaa ttaacttcaa
     1081 tacaggacat aaattattat tattctgaag aagtaacgga tatcacttcc gcaggtatcg
     1141 taagttacaa gatcacattt gacagcataa ctattatttc aaaccttgca tccggtgatt
     1201 cttcagtcag tcttacttat aattccaata taaaagattc catttattcc aagcctgatt
     1261 tcctgcagta caatgcgata atggctgagg atttttatgc aagagtagga cagaatggag
     1321 agatctctga tatatacgga cttgaaaaag tttatgaaaa aatgtttaag gcattaggag
     1381 acacactttc aaatgagcag aaagaatctc tgaaagattc attcggaaga gatgctatca
     1441 aagcagtact gcagcagcag tttcagatgt ttcctgaatc ggcggtttat cttgattctg
     1501 tatggacgag atcttacgag acacagatgc tgatcttccc tgtcagaaat tctctgagct
     1561 ataaattaaa tgaagtcaaa gaagaaaata atcagaccat acttactata gatgccgacc
     1621 tggctgttga tttcattgag aaagaaatca aagataaaca gatgagttat aaagtagaaa
     1681 attctgagac tggtggtaaa ggactgatcg tttataattt gactaaaggc tgtgtagtta
     1741 aaaaagagac gagtacaaat ctgaaacttg atatgaaaat ttcagcaggc agtcagtctg
     1801 taaatacaac acaggatgtt actactgaat tgtttgtaaa tcttattcaa taaattatat
     1861 aataagtaaa tttgaaaaaa cttaagataa tagatcatcc gttagttcag cattatctct
     1921 cgatcatgag agacaaaaaa acttcgcagg tagatttcaa agaatcactt gataaaattt
     1981 catacatact tgcgactgaa acattcggat ctctaagtct tgagaacaaa gcagttttca
     2041 ctccatttaa aaagattgaa gggaagaagg tcagcgacac tgttatcctt cttcctattc
     2101 tcagagcagg tctggggctg acaaaaggtt ttacagatct ataccctgaa atactaacaa
     2161 gtcacattgg aatttacagg gatgaagaat ctcttaaacc agttcaatat tatttcagat
     2221 ttccgaagat tgaagataaa aaagaagtga aagttattat tttagatccg atgatagcaa
     2281 caggcgggag tgttattttt accatccagt atcttttaaa tatgggtatc aggaagatca
     2341 aagttgtttc acttctctgc gctcccgaag gcattgaagc cattgaaaaa agatttggta
     2401 aagatgaaaa gaagcatctc gaaataatca catgcagtct tgatgaaaag ctgaacgaaa
     2461 aaggatatat cgttccgggt cttggtgatg caggtgacag gatctttggg acataattta
     2521 aatttattct tttccacctg atctcaaatc tttttcacag agaaaatttt tgcagcaatt
     2581 cttaccttca actatcaaat tcagtatttc aaattatcaa acaattttaa gtttttttca
     2641 gttgttcact ttctcataat cttttgttaa attgagttaa caaaatatca ctcgacagat
     2701 ttatgcttaa taatttaaat aaaaagaaat tagatgacct gctttatcac tgcagaaaaa
     2761 atttgcctgc aggtctctct aatgaaaagc tgatcacgca tgcatttaag tttgcatacg
     2821 aagctcataa gaatgcgaaa cgtatgtcgg gtgagccatt cttttcgcat ccttatgaag
     2881 ttgctataat tcttgcgcag gaaattcctc tggacgctgt atcaattgct gccgcattac
     2941 ttcatgatgt agtcgaagat acgccgttta cattgaatga tattgaggct gagtttggaa
     3001 aagagattgc agatatagtg gacggtgcaa caaagatcga aggcatgttt gaaaattatg
     3061 aaatgaaaca ggtcgagtct tataagaaga tgcttctttc aatgacttcc gacataaggg
     3121 tcattcttat aaaatttgct gaccgacttc ataatctccg aaccctggaa tttttgtcaa
     3181 acgaaagaca ggtaagactt gcacaggaaa ctctcgaaat atttgcgccg cttgcacaca
     3241 ggtttggatt gtcatctgtt aagactgaac ttgaagatat gtcattcaaa tatctgaacc
     3301 gaactgctta tgatgagatc gcaaaaaagc tgcagtcaaa aagacgtgaa cgtgaaaaga
     3361 caattaagaa gtttattgaa ccaattaagc agagattaaa tgaagacgga tttaaatttg
     3421 aaatttatgg acgggctaaa catttataca gcatatataa aaaaataatc agtagaaata
     3481 aatcatttga ggaattgttc gatctctttg ctatcagaat aattctcgat acaaaaaata
     3541 aaaatgactg cttctctgtt tacgggattt gttctgaaat atatattcca gtaccggaga
     3601 gatttaaaga ttatgtttct ctcccaaagc agaacagcta tcagtctatt catacaactc
     3661 ttgtaagtaa agaaggaaaa atgtttgagg tacagatccg cacaagagaa atggatgaag
     3721 tagctgaaaa aggtattgcg gctcactgga aatataaaga gaataataac attcacgata
     3781 aaaagcttga agactggatg aagacaattc gtgaaagcat agaaaattct tccaaagatg
     3841 agacttcatc tgaagtaatg gaaaacttta agctgaatct ttatcaggat gagatatact
     3901 gtttcactcc aaaaggtgat ctgaagatac ttccatcagg ggctactgct attgactttg
     3961 cgttcgatat acattcacag atcggaatga aatgcatcgg agcaaaagta aacggtaaga
     4021 tagtgagtct tgatacacat ttacaaagcg gtgatcagat cgaaattctt acttccaaaa
     4081 accaacatcc aaaagctgac tgggaaaaat ttgcagtaac tcataaagca aaatctgata
     4141 ttagaaaatt tcttaatact gaaaaaagaa attttattca aaaaggaaaa gaactctggg
     4201 aaaagaaatc aaaaaaatca aaggtcaaac tatctgatga agatcttaca aaagtagtac
     4261 ataaattcaa gtataaagat acaggcaatt tctttcatgc aatttctatt gatgaatcca
     4321 agatcaatca ggtgattgac tttataagtg ataaaaccaa acttataaca ctggatcaga
     4381 aactcactgc acctgaagat tataaaggaa cagaggaaga aaattttgat aagtacgtag
     4441 atgaagccag gacttctgct aacggaatat tcattggtaa cggtacttcg cagaatatca
     4501 aaggaataaa atacgaatat gcaaaatgct gtaacccaat tcccggagat gaagtgattg
     4561 gatttatcag taaagaaaaa ggtatccgca ttcacagaaa gacctgtaaa aatattttaa
     4621 acttgttttt aaaagagccg gaaaggattg ttcagatcaa ctggaatgaa gcagagtcag
     4681 gtgaatttac aggcggagtc aagatcatag gagaagacag acccggtatt cttaatgaaa
     4741 ttacaaaaac aatttcaaaa aatttcaata ctaatattaa aagtgttgat ataaagacca
     4801 agagttctat gtttgaaggg actctgatct taaatattca aaatttaaaa cagctaaatc
     4861 agattattga taaaattact caacaggaag gagtgttcag tgttacgaga tttgaacttt
     4921 aaattattca tacctttatt tttattttca gtgacatttt tattttcatt taaacagact
     4981 gagacagcag aaaataaaac agcatcgatt tcaaaagatg attcacttat cctaccggga
     5041 gaaatacatt ttgaaaattt aaaacaactg acatttggag gacagaatgc cgaatgttat
     5101 ttctctcctg acggaacaaa gttttctttt cagtcgacaa acggtgatct gaactgcgat
     5161 cagatattta caatggacct tgaaggcaat aataaaaaac ttgttagtac cggaaaaggc
     5221 acgaatacct gcgcttatta tttacccggt aatactaaag ttatattttc atcaactcat
     5281 gaagcgtcag atgactgtcc gccaaagcct gatttttcaa aaggttacgt ttgggcaatt
     5341 tatgatactt atgatattta ttctgcaaat gaagacggca ctgatttgaa aaagcttaca
     5401 aatgaaaaag gttatgatgc tgaagcaact gtttcaccca aaggagacag aatagttttt
     5461 acttcgaccc gaaatggtga tatagatatt tattctatga atacagacgg aagtgatgta
     5521 aaacaactta ccgatacttt aggttatgat ggcggaccgt tttattctta tgacgggtca
     5581 aaaataattt acagaagaac aacctttgaa aatgaagaat ctgtaataga ttataaagag
     5641 cttatatcgc agggtttgat caaaccatca cagcttgaca tctggataat ggatgcagac
     5701 ggcagcaata aaaaacagat cacaaaaaac agtgctgcta actttgctcc atttctattt
     5761 cctgacggag aaaaattact gttttgctcg aatgtaggta atgaagacaa aagaaatttt
     5821 gatatattta aaataaatat tgatgggact gatcttgagc ggataacctt atatgatgaa
     5881 tttgacggct tcccgatatt ttctcctgat ggaaaaaaac ttgcattcag ctcaaacaga
     5941 aatggaagtg ctaaaggcga aacaaatgtt tttatctgtg actgggtgga ttaaaatttt
     6001 atttgatctg gtcagaatct ccggatcaga taaaatctgt tgctgacaat gttaccctgt
     6061 ggaaacagca ctatagtatt ttcatcaaat gcatattcaa atttttcttc aaagttaaca
     6121 ttgtacttag taaatccttc agcttttgct acacctttca tatttatgat atcacctctg
     6181 atcttatata aacctttagc tctgaacggt tcataactca gatcagtatc cgcattgagt
     6241 tctccgatct caatgttaaa agaatctttt gtgaatatga actgataata ttttctgtca
     6301 cttccaattt cacctgcgct ctgaagctga tttatccaga ttccttcaat atctgaatgg
     6361 attattttct ctgaagaaga gcacgacata ataaaaatta caaatattaa aattattcta
     6421 tacataattt actttccatc ctcgcaacta acaactgcac aggcatattc atcagtatga
     6481 gatatactta tttcaaattt ataaacagaa aattctgatt ctttaagatg tacaatatat
     6541 ggtttgcctc tttcgttatt caggatctca atatctttcc agctaaaact cttaccaaca
     6601 cctgttccta tcgattttga atatgcttct tttgatgcaa acctaccggc aagatgtagt
     6661 tcaggttttg aaaatttcat acaatattta atttcatttt cggtcaatat tcttttgaga
     6721 aatctgtctc cgtaatcatc cattattttt ttaattctct taacttctat aatgtctgta
     6781 ccaagtccaa ttatcatttt agttttttat atatcactat tttctttttt aataatttca
     6841 atcagttctg atattgaatt tatgtcgtaa tctgattcag gattgacggt atcgaaagta
     6901 tctccgtatc tggcaaaaac tgttttcatt ccgacagctt ttgcacctac catgtcacgt
     6961 tcagcccagt cccctatcat cagacattca ttcgctttta atcccagttc ttgcaatgcg
     7021 agattaaacg gcacaggtga tggttttcgt tcccttgttt catcataagt tatcactaca
     7081 tcaaataaat gttggaaatt tatgtaagat aatctgagcc aggcttcctt tgaaggggcg
     7141 tcagatacta cagcaagttt cagtcccatt ttaattaatt ctatgagagt tggtaaaaca
     7201 cccggatacg gcttcagtgc agcctccctt gcttttctgt aagcaactat tcctgccgac
     7261 ataattttat aatccacttt tccaagcgct tcatgcaaaa gattatcaaa gacctgctga
     7321 tattcaattc cctcggattc ataaatctta tttatcatat ccagagtttc agcatgtgaa
     7381 agctttaatc ctgcgtctat catggcataa attgctgcat tgaccgctgc tctttttagc
     7441 agcataaagt ccaccagtgt attatccaga tcgaaaatta tggctttgat caaaatattt
     7501 taatttaatt ataaaataat tcagactttg actctgcatt ctccgcatat tccgaaaaat
     7561 tcaagtgaat gagaaatctc agtgtatcct ttttttgtta aaaatttttc tatgttatca
     7621 agaatacatg tttctacatt ctcaactttt ttgcaattct tacagacgat atgatggtga
     7681 tgatgtctgt cataaattaa ttcataacgt ttgaattcat ctttaaaatc gatcagactt
     7741 acaatgttca actcttcaaa taaattcata gtcctgtaaa cagttgccag atctatagaa
     7801 tcgtcaatca gttttcccga tatttcagaa attgtaagcg gcttatgatt ggacttcaga
     7861 attttcaata tctctaaacg aggttttgtt atcttttgag aatgtgaatt caatataagt
     7921 ttgaactctt ctgtatcgct gtcatattta ttctgaaata ttttcttctt catttttcat
     7981 aattataaaa tcccctgccc gtctttcttc ccagcagatt ggcatcaacc atttttttct
     8041 gtataaagct cggacggtac tttggatcat taaaatatgc ttcatacaca gatttcgtta
     8101 ccgaaaaatt cacgtcgatt ccaataagat ccatcagctc aaacggaccc atcgcaaatc
     8161 ctccgcatgc tttcattaat ttatcaatcg tctccgcatc tgatgtaccc tcgctgagta
     8221 tttttaaagc ttctccataa aaaggtcttg ctattctgtt cactataaaa gccggtgtat
     8281 ccttagcaac gacaggtacc ttattcattt tttttgttaa ttctacagta gcttttaaaa
     8341 cttcatcaga tgtaaaatct cctttcacaa tttcaactaa tttcataatg tgagccggat
     8401 taaaaaaatg cattcctatt actcgctcag gtcttgtctt aactgccgta gaaatatttg
     8461 taacggaaag tgatgatgtg tttgtagaaa gtattgcatc ttctctgcag atcttatcaa
     8521 gtcgtccgaa tatatccttc ttcagatcaa tgttctcaag cgctgcttct attataaagt
     8581 cacattcact taggtcatca aattctgagg taaatgacaa tctcttcaat gctttcgatt
     8641 ttttctcttc cgtcaatttt ttcttttcaa ctgattttga aagatccttt tcggttaaac
     8701 tttttgaaga attaactatt tcatccttta tatcataaac aattgtatcg atctctgata
     8761 atgctaagca tatcgcaatt ccccttccca tagtacctg
//
LOCUS       DAORVQ010000142         8700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4529_length_8700_cov_4.421053, whole genome shotgun sequence.
ACCESSION   DAORVQ010000142 DAORVQ010000000
VERSION     DAORVQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_8700_cov_4.421053"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..306)
                     /locus_tag="PLX80_10785"
     CDS             complement(13..306)
                     /locus_tag="PLX80_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HQY21316.1"
                     /translation="MAKNNSYDEFDEFEQSNDAAPSKDVLKEYINVLRQNLIPILIIL
                     SVSIIYTLIYVNTATNIYRSTTSVKLESQQGNILTANFGFEGASTTNEKYIMV"
     gene            complement(380..910)
                     /locus_tag="PLX80_10790"
     CDS             complement(380..910)
                     /locus_tag="PLX80_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21317.1"
                     /translation="MFFTVCLSGNAYSQLDSDYVKIGPEELLKPGAGNYFNFSDKNKV
                     NIEVILLGGSSPGKYLLPQGSTLLDFLIMSGNTKLDFSDDVKILRLKTETPILQARSY
                     KQFEFDKFYNDDPEEILMAYKNPLLEPGDLLILPEVKTDQNIFFYIRETVYFIGTLIS
                     FYYLIDNLVRRTITVN"
     gene            complement(1088..2029)
                     /locus_tag="PLX80_10795"
     CDS             complement(1088..2029)
                     /locus_tag="PLX80_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY21318.1"
                     /translation="MNSTKFLVTGGAGFIGSNIVSELLKQGHSVRVLDNFSSGKRENL
                     KNFDKDFELIEGDIRSYHIVSEALKDIEVVLHQAALPSVPRSIKDPITSNEVNVNGIL
                     NILEASLSANVRRVVYASSSSVYGDNPELPKHEGMLPNPLSPYAVSKLAGEKYCSVFS
                     KIYGLETIALRYFNVFGPNQDPSSQYSAVIPKFITAMMKNERPVIYGDGEQSRDFTYV
                     SNVVNANILAAFAKCNTGIAMNCAYSGRITLNELVRSLNKMLNKNIEPIYEKQRPGDI
                     KHSFADIKLAKEKIGFNTTIDFETGLKNTLDFYIKKN"
     gene            complement(2236..2769)
                     /locus_tag="PLX80_10800"
     CDS             complement(2236..2769)
                     /locus_tag="PLX80_10800"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HQY21319.1"
                     /translation="MIFHRKMMTETHDKNWYVMQTKPRNEIKVFNNIEYRGIEAFLPL
                     VEKVKIWSDRKKKVNEPLFSGYLFVHANEYERVHAIKENFGAVRYIYYENRPAVVNER
                     EIQIIKQALLEPEKISVEEKHIKMGDIVKINHGIFKGMMGYVNEIRGNYKLTVNLEEF
                     SYSISIILNSNEVSTLK"
     gene            complement(2775..3308)
                     /locus_tag="PLX80_10805"
     CDS             complement(2775..3308)
                     /locus_tag="PLX80_10805"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQY21320.1"
                     /translation="MRSISVVFFIILVIASNKIYSQSDKIKVGNDEFSSQQGGYYNYG
                     DKDKVNIEVNVWGYVKFAGKYLLPQGSTLTDLISYSGGPMTDSKLEDIRLYRPKNDSL
                     GISKDQIIKLNYNDLVWEEKVNFKNKLNTVLLPGDILIITGEPRYFGRDNVGFILAIS
                     SVLISLGILVISITNNK"
     gene            3866..6175
                     /locus_tag="PLX80_10810"
     CDS             3866..6175
                     /locus_tag="PLX80_10810"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQY21321.1"
                     /translation="MFYLIASQILNKPSLNGKSNHSAVNFQEARESKDNFKVYLNILR
                     MNLIPILIITLLCVLLTVAYVMYAKDIYKSTTTIKINRPQGSVLSSSLIPEFQDFITD
                     RYISNEIEVLKSYKIREIVAENLIDTFKIDNDKAKFYYILNHGDNEDVTALISVSSLA
                     DILSVIVKIDQKRGLDVVDIVVESPAKYEAMLIANLYSKVYQEFSLDLSREELTSLTG
                     YLKEEKENKFKDLAKAESELEDYQKDGGLILLDAQVKNVVENISMLEAQQNAARIELN
                     SKQEEYNSLKTEINRMDPTLVDFLQGKIDELYIAEIQSKIAELESKRDIEASIPEDDR
                     LKQKTLSEYNKKIEPLRETLDEKIELMRTSLFSSTPEERRMISQKLLEAGVGVQSYEA
                     KVNTLSNMLLKYEDQFSKLPTQSIEYAKLERNRKATEKLYLILEEKYQEAKINERIKL
                     GTVLVIAPGIIASKPSKPNRMLIIFMGTLLGLGLSIAFVFTKNYFDHTIKSPDEIENR
                     GISVLSWIPAIDELKNKSFQSEFIVANKPKSPPSEAFKALRTRIQYSKLEETPLKTIL
                     LTSSVPSEGKTTISLNLAGSLAQMDKKVLLLDCDLRKPRVHNVFEIDRFPGLSDYLFS
                     NASLEEITRKTKLENLEVITSGTIPPNPSEMLSSKQMKDFIEKMKGIYDYIIIDSPPY
                     ISVTDAEILFRIADGTILVLKAGSTPSDEFYRTSERLTNLDPHNFLGVVFNNFSFKSA
                     YGYYYNYYYYYTRPDDKSMSGKSTSAAEN"
     gene            6249..7556
                     /locus_tag="PLX80_10815"
     CDS             6249..7556
                     /locus_tag="PLX80_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQY21322.1"
                     /translation="MNLKSKVINNDFTVGIIGLGYVGLPLALEFAHKGIAVIGFDLDE
                     FKIKKILKEKSSYIKHIPSEKIKEAVNNKKLTATTDFSKLPEADAVIICVPTPLNVNR
                     EPDMSYVVNTAETIFKYLRKGQLVSLESTTYPGTTDEILLKIFESKGMKVGKDFYLCF
                     SPEREDPNNEKYSTATIPKVLGGVTKECIDIGKLIYEKVIVKVVPVSSTKAAESTKLL
                     ENIFRSINIALVNELKVVFDKMGIDVWEVIEAASTKPFGYTPFYPGPGLGGHCIPIDP
                     FYLTWKAREFEISTKFIELAGEINTAMPEYVVSKVYHALNENKKCVNGSKILLLGLSY
                     KKDIDDLRESPSLKLIELLQDEGADVDYSDDYVTMIPKLRKYDFNKKSVALTKKNIAK
                     YDLVLLSTDHSYYDYKLIYDNARLIVDTRNAFKKFKGKKLFKA"
     gene            7581..8555
                     /locus_tag="PLX80_10820"
     CDS             7581..8555
                     /locus_tag="PLX80_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY21323.1"
                     /translation="MSEIKLAIIGCGKWGMNHVKTAYKLFGDDLKYCCDFSDDSAEKV
                     RAVSDRIIFTKDPNDIILDTDVTAVIIATPAETHFEIAKKMLLAGKHVLVEKPITLNS
                     EDAHILNELALENDRILMVGHLLLFHPAILKIKEFVDEGKLGKLQYIYSNRLNLGTIR
                     TEENILWSFAPHDISVIQYLVDDIPEEVTATGAVFLTSEIQDTTLTNLKFKNNIHAHI
                     FVSWLHPFKEQRLVVIGDKSMMMFEDSIPTDKLKLYKKGIDMVNGVPVKRDSDYESVE
                     FGDDSPLELEQLHFIDCIKNSTTPRADGRNAIEVLETLERAQKQLVNS"
BASE COUNT         3028 a         1386 c         1390 g         2896 t
ORIGIN      
        1 catgacagcg atctaaacca ttatatattt ctcatttgtc gtactggcac cttcaaatcc
       61 aaagtttgca gttaaaatat ttccctgctg actttctaat tttacgctgg ttgtagatct
      121 gtaaatattg gtcgcagtat taacataaat tagagtataa ataatactaa ccgataaaat
      181 aattaatatt ggaatcagat tttgtctgag tacattaata tattccttta aaacatcttt
      241 agaaggagcg gcatcgttag attgttcaaa ttcatcaaat tcatcatatg aattattttt
      301 tgccattgct tacttttaat ttaaaacttt tataaaatat tattaaaata acctaaaaaa
      361 aaaaaatgaa aacaaaaaat taattaaccg ttatagttct tcttacaaga ttgtctataa
      421 gataataaaa tgaaataagt gttccgataa aataaacagt ttccctgata taaaaaaata
      481 tattctgatc agtttttact tcaggaagaa tcagaagatc acctggctca agtaacgggt
      541 ttttataagc catcaatatt tcttcaggat catcattata gaatttgtca aattcaaatt
      601 gtttatatga cctggcttgt agtattggtg tttcagtttt taacctaagt atcttcacat
      661 catctgaaaa atccaatttg gtgttacccg acataattaa aaagtcaagt aatgtagaac
      721 cttgaggtaa aagatatttt ccgggactgc tcccgccgag taaaatgact tctatgttta
      781 ctttattttt atctgaaaaa ttaaaatagt ttccagcccc tggttttaac aattcctcag
      841 gtcctatttt gacataatcg ctgtcaagtt gagaataagc atttccagaa aggcaaactg
      901 taaagaacaa tattagtaaa aacgtaatgc gatgtagatt caaaatttta tagtctgtga
      961 atttgtaatt gaaaaaattt tctaaatatt ttatccgttc ttttgtttgg taacataaga
     1021 aattaatcta taaattctta gtcataaaat tatatttaaa gagggattat atatttttat
     1081 tttattttta gttttttttt atatagaaat ctaaggtatt tttcaaccct gtttcaaaat
     1141 caattgtagt attaaatccg attttttctt ttgctaattt aatatctgca aaagaatgtt
     1201 ttatatcacc tggtctttgt ttttcatata tcggttcaat atttttattt agcattttat
     1261 taagagacct tacaagttcg ttcagagtaa tccttccgct ataagcacaa ttcattgcaa
     1321 tccctgtatt gcattttgca aaagctgcaa gtatatttgc atttacgaca ttagaaacat
     1381 atgtgaaatc tcttgattgc tctccatctc cataaataac cggtctttcg tttttcatca
     1441 tggctgtaat aaattttgga attacagcag aatactggga tgaaggatcc tgattcggac
     1501 caaatacatt aaaatatctt aaagcaattg tttcaagtcc gtagattttg gaaaatacgc
     1561 tgcaatattt ttcacccgca agttttgaaa ctgcataagg tgaaagtgga tttggaagca
     1621 tcccctcatg ttttggtaat tccggattat caccatacac agatgaagaa gaggcatata
     1681 caacccttct tacatttgca cttaatgatg cctctagaat atttagaatt ccatttacat
     1741 ttacttcatt tgatgtgatc ggatctttta ttgatctagg aacagaaggt aatgccgcct
     1801 gatgtaatac tacttcaata tcctttagcg cttcacttac aatgtgataa cttcttatat
     1861 caccttctat taattcaaaa tctttatcaa aatttttaag attttctctt ttacctgagg
     1921 agaaattatc aagaaccctg acagaatgtc cctgtttcaa taattctgaa actatgtttg
     1981 atcctataaa tcctgcacca cctgttacta aaaattttgt tgaattcata cttttttaaa
     2041 tttaaaactc tgagaatact tcaaaccgat tacagctccg ctatttgact cacattaata
     2101 aaaaaattga gccatctaaa tattttattt ttaacgatct gatattgtac cgaaatttcc
     2161 aaacaataaa attaacaata tctgttttta caactataaa ttctctatcc gtatttctaa
     2221 ataaatgaac tcagattatt tcaatgtgct gacttcattt gaatttaaaa ttatacttat
     2281 agagtatgaa aattcttcca gattaaccgt aagtttgtaa tttcctctaa tttcatttac
     2341 atatcccatc attcctttaa atataccatg attgatcttt acaatatcac ccattttaat
     2401 atgtttttct tcgacactta tcttttcagg ttcaagtaat gcctgcttga ttatttgaat
     2461 ttctctttca tttacaacag caggtctgtt ttcataatat atgtatctta cagctccaaa
     2521 attttcctta atagcatgga ctctttcgta ttcattggca tgcacaaaaa gataaccgga
     2581 aaaaagtggt tcattaactt tcttttttct gtctgaccag atctttactt tttcaactaa
     2641 cggtaaaaat gcttcaatgc cacgatattc aatattatta aatactttaa tttcatttct
     2701 aggctttgtc tgcattacgt accaattttt gtcatgagtt tcagtcatca tttttctatg
     2761 aaagatcaaa ctgcttattt attgttggtt attgatatta ctagtatccc taatgaaatt
     2821 agtactgatg aaattgcaag gatgaagccg acattgtctc tgccaaaata tctgggctca
     2881 cctgtaatga taaggatatc accgggtaac aagactgtat ttaacttatt cttaaaattt
     2941 actttttctt cccaaaccag atcattataa ttaagtttta ttatctgatc ttttgaaatc
     3001 cctaagctgt cattctttgg tctgtataat ctgatatctt caagttttga atcagtcatt
     3061 ggacctccgc tgtatgatat cagatctgtt aatgttgatc cctgaggaag aagatattta
     3121 ccggcaaatt taacataacc ccacacattt acttcaatgt taactttatc tttatctcca
     3181 taattgtaat atcccccttg ttgagatgaa aattcgtcat ttccaacttt gattttatca
     3241 gattgagaat atattttatt tgatgctatt accagaatta taaaaaatac gactgatatt
     3301 gatctcaaat ttaacttaaa aattgaaaaa atataagtta ttaacataat taaaatagtg
     3361 tttaaataaa agtccgaaca agaacttagc tgacttgtct taaaataaac tttaaataaa
     3421 aatcaagata ttattttagt ttcaaatttt ttttaagaat ttccagtctt tttatataat
     3481 actaagaata taattaatta actggtttag agataattta tacttgggtt ttgacagatc
     3541 tataaaatca acataaacat aaaattaaaa aatcttacat ttacatcttt ataattcaaa
     3601 ttactttttc acattcaaaa ttatagaata ataatcagca gatttcattt caaaaaaata
     3661 attctcagat agattaattt ccaaaactgc aaattttatg tcgttgaaat aatgcttaaa
     3721 acagtatatc tttgtagtct taattataaa aaattcattt ttgaataaat ccggatactg
     3781 ctttttttaa acggatagtg taatgaaatt tatgatcgga tgttaatttt cagtttgttg
     3841 gtatatatgt ctgaaaaaat gttaaatgtt ttatttaatt gcatcacaaa ttttgaacaa
     3901 accaagctta aacggcaaga gtaatcattc cgcagtaaac ttccaagaag caagagaatc
     3961 taaagataat tttaaggtat atctgaatat cttaaggatg aatctgattc ctatattaat
     4021 tatcactctt ttgtgtgtgt tacttactgt tgcttatgta atgtatgcca aggatatata
     4081 taaatccact actaccatta agataaacag acctcaggga agtgttttga gctcaagtct
     4141 catacctgaa tttcaggact ttataacaga cagatatatt tcaaacgaga tcgaagtttt
     4201 gaaaagttat aagataagag aaattgttgc ggaaaatctt attgacactt ttaaaataga
     4261 taatgataaa gctaaatttt attatatttt aaatcatggt gataatgaag atgtaactgc
     4321 cttaatttct gtctccagcc ttgcagatat tctttctgtt attgttaaaa ttgaccaaaa
     4381 gcgcggactc gatgttgtcg atattgtagt tgaaagcccg gctaaatatg aggcaatgct
     4441 tattgcaaat ctctattcta aagtttatca ggagttcagt cttgatctgt cccgggaaga
     4501 acttaccagt ctgaccggat atttaaagga agaaaaggaa aataagttta aggatctcgc
     4561 aaaagcagaa agtgaacttg aagattatca gaaagacggc ggattgattc tgctggatgc
     4621 acaggtgaaa aatgttgttg aaaatatttc catgcttgaa gctcagcaaa atgcagcgcg
     4681 catagaactc aattcaaaac aggaagaata taattctctg aaaactgaaa ttaaccgaat
     4741 ggatccgacg ctggttgact ttcttcaggg gaaaatagat gaactttata ttgctgagat
     4801 acaatcaaaa attgctgaac tcgaatcgaa gagagatatt gaagcatcga ttcctgaaga
     4861 cgatagactt aaacaaaaaa ctttaagtga atataataaa aagattgaac ctttaagaga
     4921 aacccttgat gaaaagattg aattaatgag gaccagtctt ttttcaagta ctcccgaaga
     4981 acgaagaatg atatctcaga aacttttaga agccggggtc ggagttcaat catacgaagc
     5041 aaaagtaaat actctttcaa atatgctttt gaaatatgaa gatcagtttt cgaaacttcc
     5101 tacacaaagc attgaatatg caaagcttga aagaaacaga aaagctacgg aaaaacttta
     5161 tttaatactg gaagaaaaat atcaggaagc aaagatcaat gaaaggatca agcttggtac
     5221 tgtacttgta atagctccgg gtattattgc ttctaagcct tccaaaccaa acagaatgct
     5281 tataattttt atgggtactt tgcttggatt gggacttagt atagctttcg tatttactaa
     5341 aaattatttt gatcatacta taaaaagtcc tgatgaaatt gagaacagag gaatcagtgt
     5401 actttcatgg atacctgcga ttgacgaact taagaataaa tcttttcaaa gtgaattcat
     5461 tgtcgccaat aaacctaagt cacctccgtc tgaagctttc aaagctttaa gaacgaggat
     5521 tcaatattct aaacttgaag agactccatt aaagactatt ttacttacga gttcagttcc
     5581 ttcagaaggt aagacaacga tctctttaaa ccttgccgga agtctagctc agatggataa
     5641 aaaagtatta cttctcgatt gtgatcttag aaagccgaga gttcataatg tttttgaaat
     5701 agacagattc cccggactga gtgattatct tttttcaaat gcttcactgg aagagataac
     5761 gcgtaagacg aagctagaaa atcttgaagt gatcacaagc ggaacgatac cgccgaatcc
     5821 ttcagaaatg ctgtcttcaa aacaaatgaa agattttatt gagaagatga aaggaattta
     5881 tgattatata attattgatt caccacctta catatctgtt actgatgccg agatactatt
     5941 cagaattgca gacggaacaa tacttgtact caaagccggc agcactcctt ctgatgagtt
     6001 ttacagaact tcggaaaggt tgacaaatct tgacccacac aacttccttg gagttgtgtt
     6061 caataatttc agtttcaaaa gtgcttacgg atattattac aattactatt attattatac
     6121 cagacctgat gacaaatcaa tgtctgggaa atcaacttct gcagctgaaa attaattctt
     6181 tgtttaaaat taatttacat tccgtattat atttaatcta aaacttaaac tatatctgaa
     6241 aggaaatatt gaatttaaaa agtaaagtta taaataatga tttcacagtc ggtataatcg
     6301 gtctcggtta tgtcggactt cctcttgcac ttgagtttgc tcacaaggga attgctgtaa
     6361 tcggattcga tcttgatgaa ttcaagataa agaaaattct caaagaaaaa tcttcataca
     6421 taaaacatat tccttctgag aaaataaaag aagcagtcaa caataaaaaa cttactgcca
     6481 caactgactt ctctaaactg cctgaagctg atgctgtcat tatctgcgtt ccgactccgc
     6541 tgaatgttaa ccgtgagccg gacatgagct acgttgtaaa tactgctgaa acaattttta
     6601 aatatctgag aaaaggtcag cttgtctcac ttgaaagcac tacatatccg gggactacag
     6661 atgagattct gttgaaaata ttcgaaagta aaggaatgaa agtcggtaaa gatttctatc
     6721 tgtgtttttc tcctgaaaga gaagatccga ataatgaaaa atattctaca gctaccattc
     6781 caaaagtttt aggcggcgtg acaaaagaat gtattgatat tggaaaactt atttatgaaa
     6841 aagtgatcgt caaagttgta cctgtatctt ctaccaaagc tgcagagtca acaaaacttt
     6901 tggaaaatat tttcagatct ataaacattg ctttagtgaa tgaactaaaa gttgtatttg
     6961 ataaaatggg aatagatgta tgggaagtaa tcgaagctgc ttcgacgaaa ccatttggct
     7021 atacaccatt ttatccgggt ccgggactcg gcggtcattg cattccgatc gacccttttt
     7081 atctaacatg gaaagcccgt gagtttgaga tctccacaaa gttcatagag cttgccggtg
     7141 aaataaatac tgccatgcct gagtatgtag tatcaaaagt ttatcacgcg cttaatgaaa
     7201 ataaaaaatg tgtgaatggt tctaagattt tactgcttgg actttcttat aaaaaagata
     7261 ttgatgatct gagagaatct ccttctctaa aacttattga acttctgcag gacgaaggtg
     7321 cagatgtaga ttacagtgat gattatgtta ccatgatccc gaaattaagg aagtatgatt
     7381 tcaataaaaa atctgtcgcc cttacaaaaa agaatattgc aaagtatgat cttgttttac
     7441 tttccactga tcattcatat tatgattaca aattaattta tgacaatgca aggctgatag
     7501 tcgatacgag aaatgcattt aagaaattca aaggaaaaaa attattcaaa gcttaactaa
     7561 attaatatat tgagaaaatt atgagtgaaa taaaattagc aataatcggc tgcggcaagt
     7621 ggggcatgaa tcacgttaag actgcatata aactttttgg agatgatctt aaatattgct
     7681 gcgacttttc tgatgacagc gcagaaaaag taagagctgt atctgataga attatattca
     7741 caaaagatcc taatgatatt attttagaca ctgatgttac agcagtcatt attgccacac
     7801 ctgctgaaac gcattttgaa attgcaaaaa aaatgctgct ggcgggaaag catgttttgg
     7861 tggaaaagcc gatcacactg aattccgaag atgcgcatat tctgaacgaa cttgcattag
     7921 agaatgacag aattttaatg gtcggacatt tacttttatt ccatccggct atacttaaga
     7981 taaaagaatt tgtcgatgaa ggaaagctcg gtaagctgca gtacatctac agcaacagat
     8041 taaatctcgg cactataagg acagaggaaa acatactatg gagttttgct ccgcatgata
     8101 tttctgtaat tcaatatctt gttgatgaca ttcctgaaga agtaacggca acaggcgcag
     8161 tatttcttac cagtgaaatt caggatacaa ctctgacgaa tctgaaattt aaaaataata
     8221 ttcacgcaca cattttcgta agctggcttc atccattcaa agaacagaga cttgtagtca
     8281 tcggtgataa atcaatgatg atgtttgagg attcaattcc gactgataaa ctgaagctgt
     8341 ataaaaaagg aattgacatg gtaaatggtg tgcctgtaaa aagagattcg gattatgaaa
     8401 gcgtagagtt tggggatgat tcccctcttg agcttgagca gcttcatttt attgactgca
     8461 taaaaaactc aactacaccg agagctgacg gaaggaatgc gatcgaagta cttgagacat
     8521 tggaaagagc gcaaaagcaa ttagttaata gttaatagtt aatagttagt agttagtact
     8581 taatagttta gaaattttac cacgaagaca cgaaaacaca aaatagaaaa agtatttaag
     8641 aaattagtta aaggaaaaca aaagaattgt ctttccgcgt tctcccggcg ggaatctaat
//
LOCUS       DAORVQ010000143         8550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4660_length_8550_cov_5.968217, whole genome shotgun sequence.
ACCESSION   DAORVQ010000143 DAORVQ010000000
VERSION     DAORVQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_8550_cov_5.968217"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1769)
                     /locus_tag="PLX80_10825"
     CDS             complement(222..1769)
                     /locus_tag="PLX80_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY21324.1"
                     /translation="MANTKQPNILFIMADDIGMWNIGAYHRGVMAGRTPHLDKLASGG
                     AILTDYYAEASCTAGRASFITGELPIRTGLTTVGQAGATVGMPAEAPTIATALKELGY
                     ATGQFGKNHLGDRNEYLPTLHGFDEFFGYLYHLDAMEDPFHPNYPEELKNTVGPRNVL
                     HTWATDTDDATVDPRWGKVGMQKIEDKGPLPPHPTDGIELNMETVDDVILEHAENFID
                     KAVKDDKPFFCWLNPTRMHVVTHLSPKYEALRTSENSWSLQEAGMSQLDDIVGHVTKK
                     LDDMGLTENTIIVFSTDNGTESFSWPDGGTSPFKGAKGMGTEGGFRSPCIVHWPGTVK
                     PNSVINGVMSGMDWFPTFVSAAGNSNIAADLIAGKSLNGKDYKVHLDGYDQTALLSGG
                     ESARKEFFYFTESTLAAVRIGDWKYTFIDQPQGWFGPKVKLDWPGLVNLRLDPFEKMG
                     MDGSMYFGEWFTYEFWRFVFVQQQVAKLAETALEFPPMQAAASFNLDAVKQHIQQAMA
                     KRGSTGN"
     gene            complement(2182..4077)
                     /gene="asnB"
                     /locus_tag="PLX80_10830"
     CDS             complement(2182..4077)
                     /gene="asnB"
                     /locus_tag="PLX80_10830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQY21325.1"
                     /translation="MCGINGILYFNNYQSQEDKTFYISRIGLMNKAIAHRGPDGDGVF
                     VNYPVSLGHLRLSIIDLSENAAQPMFNEDKSVVLIYNGEIYNYKELISDLKSKGHIFR
                     SECDAEVVIHSYEEYGYDCVKKFNGMWAFSIYDIKKNIFFASRDRFGVKPFYFYKDEN
                     EFIFSSEIKAILKVKEIHEANLAKVYEYLAYGYRTTDGNTFFKDVNELKPATNLIVNL
                     ENSELKFVKYWDFPGYINPDNSDARENLKELLYDSVKIRFRSDVPVSILLSGGIDSGV
                     ITKITDELIDSGNLPNGNVSAFSAVFPGYEFDETKEIDEIISRCRHIDSYKLSPSGED
                     LTSSINDFIYGMGEPVFSTTSFAHYKLMKEIHNKNVKVVLNGQGSDEAWCGYGRYIAG
                     YFLLDRLLSEPGKFLPQLNSINRKLEMSYVQLISQTLKAFVGRRQASLVRSKNIEKTR
                     EIISDELYNDNYLNFPDTRFNSPNGDNLSAYMKNNIAFQGFGQILHYEDHSSMQSSIE
                     IRSPFIDYRIMELAFSIPEKMKFHDGVTKKILRELFKDKLPSSVIDNHRKIGFMTPFD
                     KWLGEESSVKFVNEILNSSSFNNKNIWNAEKIRKIFNSKEKYPSFPYWRILNLELWSQ
                     VYRIKNL"
     gene            complement(4080..5297)
                     /locus_tag="PLX80_10835"
     CDS             complement(4080..5297)
                     /locus_tag="PLX80_10835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY21326.1"
                     /translation="MSECFPNKNKPFTGEFVYRQAKALSEHCKVVMIVPLRYMPGREI
                     LTGDSEGLFKNFNNWLSGIKDTSDYSEGNLRVIYFGYISLPRPFFESADNTFINFFFY
                     NKLKKLITEQNPDLIYCNWIRPWAPVTAGITKELNIPFVIDHHEDIPTLKKLFPDKAS
                     QFLQVFKSADKIIVHSGLNKKQLVNEKLTEREIKVIHLGQNFDVSITEKYFDNSNLRL
                     ICVSHLTEKRKNIDVLIKAVSLLDKKIDFELTVIGDGVFKNEYMDLATSVGQKEKVFF
                     KGALSHEDIGAELDVSDIFILPSFPEAFGIVMTEALAKGVPVISCHGNGGAEELKALG
                     YPVILVPPENEYELAKAIEDLSNNRSEMKEMSEKGKIIVSDNFTWDMNGISTYKFLDN
                     TLRNFINKMISRK"
     gene            complement(5554..6597)
                     /locus_tag="PLX80_10840"
     CDS             complement(5554..6597)
                     /locus_tag="PLX80_10840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21327.1"
                     /translation="MLGEQDLGPLEFYLAEALRFHGKNVNYYNIHNFYPDYWGKLNVY
                     SHRLPRRFDNSIKNKFIKIINDEILKKYRSEKPSHIFIYNDCLVMPDTLDYIKNNGTK
                     VIVFLGDDPNILYPSKKSFLLTVMNADLIITPDSGWIDGLKMLDISDDKIFFTPVGTD
                     ENTFFPFTPSNDQLQKYQTDILFVGTGYFLNAWGIKRAAVLNELSDFNMKIFGDKLWY
                     ELFPYFPKLEKHFTNIPLSAKEVNIASNCAKIYPVVVNSGVINGVSTRIFDGIASGIF
                     ILSEYKKDFDNFFPDGEVVCFKSKKELKDKAEYFLKNENEMIDMTKKARELVMKKYTL
                     KTITKNILDSLDS"
     gene            complement(6608..7948)
                     /locus_tag="PLX80_10845"
     CDS             complement(6608..7948)
                     /locus_tag="PLX80_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY21328.1"
                     /translation="MINLLIYFLLTSVLVLLLYNYKSGIVTCLMVITNFGEFIYVNPN
                     LEIGDFGGIGTIYFMDVFFLALLIVVLLKISELKNYNYKLSALIFFILCSIAFVIPLL
                     INSFSLKDLISVIRPFGNLLFLPYLVVSIRDIKQFNYFEKTIVFLVFFFIVIQVTEYI
                     SQKRIPFRLFESDSLFYGEDPFSVEFGGIKTGYIWSRIGYLLPFNLLFGCYYFFSEKR
                     NYGLLLLTAYLLSIMIALSRIWIIGFAFFLLTISIFFFLKKDSRENVRLKLIAFIGSF
                     ALIGVILLFTSSTFNQIFDIFLLRVNSINDLADKTDSSFLGREYILFQMISIWSEYPL
                     FGTGFSSISRNLVTNDLGFPNIVTIFGTSGIFLFSVFFYQYYKNIKPFIQEHYILFVS
                     LASVMLMIIFMSIFSIDMFYFNATGAILMAIGNILYNISKQEDSTEEAGYNYAA"
     gene            complement(7949..8491)
                     /locus_tag="PLX80_10850"
     CDS             complement(7949..8491)
                     /locus_tag="PLX80_10850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQY21329.1"
                     /translation="MIKYLDLGFRSVERKDIELIRKEHNDESVLLMLKDPFIITETNQ
                     EEWFNNITRNKHNTVYCMFKDEPDQVIGVWKLQGLDMTNRNTEMGMEIFSVYRGKGYS
                     LKCFRMIFKYLFETLNIHTLHAKIGEYNGASLKTAQRAGFRITGKIPESLFRNGKYYD
                     DIVLAITYSDYMENIKPNVS"
BASE COUNT         2768 a         1568 c         1363 g         2851 t
ORIGIN      
        1 tgaaggcagg cgcttaacca tttcttcatc aattttattg ccactgtatt ttttacttta
       61 ttaatatttt ttacaatttt atatactttg aatttttact tctattaaaa aaagtgtgac
      121 actttaaaaa aaaccatttt aaaaagtgcc acactgatat tttatatttt atattttaaa
      181 tttaacataa caaatttaaa ctattaacta ttaactattt actaattacc ggtacttcct
      241 cgttttgcca tagcttgctg aatatgttgt ttaaccgcat caagattgaa acttgctgct
      301 gcctgcatcg gtggaaattc aagtgcagtt tcagctaatt ttgctacctg ctgctgaaca
      361 aagacgaaac gccagaattc ataagtgaac cattcaccga aatacataga accatccatt
      421 cccatctttt caaacggatc taatctcaaa ttaactaatc ccggccagtc taattttact
      481 ttaggtccaa accatccctg aggctggtcg ataaatgtat atttccagtc accaatcctg
      541 actgcagcca gagtgctctc tgtaaaatag aagaattctt ttctagcaga ttcccctccg
      601 gataaaagag ctgtctgatc atatccgtca agatggactt tgtaatcttt tccattaaga
      661 gatttacctg ctattaaatc agcagcgata tttgaatttc ctgctgcact gacaaaagtt
      721 ggaaaccagt ccattcctga cattactcca ttgatcactg agtttggctt aactgttccc
      781 ggccaatgaa caatacatgg agatctgaat ccgccttctg ttcccattcc tttagcacct
      841 ttaaatggtg atgttccacc atcaggccag ctaaagcttt cagtaccatt atctgtagaa
      901 aagacgataa tagtgttttc tgtgagaccc atatcatcaa gtttcttagt cacatgtcca
      961 actatatcat caagctgaga cataccggct tcctgcaaag accaggaatt ttcagaagtt
     1021 cgaagggctt catactttgg agataaatgt gttaccacgt gcatgcgtgt agggttaagc
     1081 cagcaaaaga aaggtttatc atcttttaca gctttgtcaa taaagttttc tgcatgttca
     1141 agaattacat catctacggt ctccatattt agttcgattc catctgtagg atgtggagga
     1201 agaggacctt tatcctcaat tttctgcatt cctacttttc cccatcttgg atctactgtt
     1261 gcatcatcag tatcagtagc ccatgtatgt aaaacatttc ttggacctac tgtgtttttt
     1321 aattcctccg gataattcgg atggaaggga tcttccattg catcaagatg ataaagataa
     1381 ccaaagaatt catcaaagcc gtgaagtgtc gggagatatt catttctgtc tccaagatga
     1441 tttttaccga attgtccggt agcatatccc aattctttta aagcggttgc aatagtaggt
     1501 gcttcagcag gcatacctac ggtagctcct gcctgaccaa cggtagtaag tcctgtacgg
     1561 ataggaagtt caccggtaat aaaagaagct cttcccgcag tacagcttgc ttctgcgtaa
     1621 taatctgtca agattgcacc accagatgct aacttatcaa gatgaggtgt gcggcctgcc
     1681 attacacctc tgtggtacgc gccgatgttc cacataccga tatcatctgc cataatgaac
     1741 agaatattcg gttgttttgt gtttgccatt ttcgtttttt tttttagtta gtaaatatga
     1801 ttaatatatt tttgaaaaaa aaattttcat tcacgaaata aaaagtaagt aaattgttga
     1861 tttttactaa gggtaattat tgtatgataa ataaaattta gaatttttaa atagattgaa
     1921 actggaatgg gataaaattt gatccttact aaatttgggg gttaatataa tagtaaacaa
     1981 ttaactatga tctaataact tttttccatt caatttctaa caaaataatt tttatcactt
     2041 ttacaatcaa gtattaaaat aaaatctaaa aataaattta tgtaaactaa tatatatttt
     2101 taaattaaaa atttattatc actattaatc tattattcaa agggtgtaca aataaaactt
     2161 tattaagtgc agtaatttgc atcacagatt ttttatccgg tatacctgcg accaaagctc
     2221 aagattgaga attctccagt aggggaatga cggatatttt tcttttgaat taaaaatctt
     2281 tcggatcttc tcagcattcc atatattttt attattgaat gaagaggaat ttaaaatttc
     2341 atttacgaac tttactgaac tctcctcgcc aagccactta tcaaacggag tcatgaatcc
     2401 aatcttgcgg tgattatcta tcactgatga cggcagctta tctttgaaaa gttcgcggag
     2461 aatcttcttc gtaactccgt catgaaattt cattttttca ggaattgaaa atgccagctc
     2521 cataattctg taatctatga aaggcgatct tatctcgatc gagctctgca tggaagagtg
     2581 atcctcataa tgtagtatct gcccaaatcc ctgaaatgct atattatttt tcatatatgc
     2641 actgagatta tctccgttag ggctgttgaa tcttgtgtcg ggaaaattca gataattgtc
     2701 attataaagt tcatcagaaa ttatctcacg ggttttttca atattcttcg atctgacaag
     2761 tgacgcctgc cttctgccta caaatgcttt tagtgtctgg ctgatgagct gaacataact
     2821 catttctaat tttctgttta tagaatttag ttgcggtaaa aatttcccgg gctcactaag
     2881 gagtctgtca agcaaaaaat atccggcaat gtatcttccg tatccgcacc acgcttcatc
     2941 cgatccctgc ccgttcaaaa ctaccttcac atttttatta tgtatctcct tcataagttt
     3001 gtaatgcgca aagcttgtcg tactgaaaac aggttcgccc attccataaa taaaatcatt
     3061 aatcgatgaa gtaagatctt ctcctgaggg agaaagttta tagctgtcaa tatgccggca
     3121 tcgtgaaata atttcatcta tctcttttgt ttcatcaaat tcataaccgg gaaatacagc
     3181 agagaaagcc gaaacatttc cgtttggaag gttacctgaa tcaataagct catcagtgat
     3241 cttcgtgatc acacctgaat caatgccgcc gctgagcagt atcgatacag gaacatcact
     3301 tctgaatcgg atcttaacgc tgtcatataa aagttctttg agattttctc tggcatccga
     3361 gttgtcggga ttaatatatc caggaaaatc ccagtacttc acaaatttca attcggaatt
     3421 ctccaaattt actatcaggt tcgtcgcagg tttaagttca ttcacatcct taaaaaaagt
     3481 attaccgtca gtagttctgt atccgtaagc gagatattca taaacctttg caaggtttgc
     3541 ttcatggatt tccttcactt ttagaattgc ttttatctca cttgaaaaaa taaattcatt
     3601 ctcatcttta taaaaataaa acggctttac accaaacctg tctcttgatg caaaaaaaat
     3661 attcttctta atatcataaa ttgaaaatgc ccacattccg ttaaattttt tgacgcagtc
     3721 atatccgtat tcttcatagg aatgaattac aacttcggca tcacactccg atctgaatat
     3781 atgtccctta cttttcagat cagaaatcag ctctttgtaa ttatagatct cgccgttata
     3841 tataagtact acagacttgt cctcgttaaa cataggctgt gcagcatttt ccgaaagatc
     3901 aataatgctg agtcttaaat gtccaagaga gacaggataa tttacaaata ctccgtcacc
     3961 gtcaggtccg cgatgggcaa tagctttatt catcagaccg atccgagaga tataaaaagt
     4021 tttatcttcc tgagactgat aattattaaa atacaatatg ccgtttatgc cgcacaaaat
     4081 tattttctgc tgatcatttt atttatgaaa ttccttaatg tattatctaa aaatttatat
     4141 gtgctaatac cgttcatatc ccatgtgaaa ttatctgaga caataatttt acctttttct
     4201 gacatttctt tcatttcaga tctgttatta gaaagatctt caattgcttt tgccagttca
     4261 tattcatttt ctggagggac aagaataaca ggatatccca atgcttttaa ttcctccgca
     4321 ccgccatttc catgacatga aataacggga acaccttttg caagagcttc agtcattaca
     4381 ataccgaatg cttcaggaaa actcggcagt ataaatatat cagatacatc aagctctgca
     4441 cctatgtctt catgagacaa ggcacctttg aaaaatactt tttccttctg accgacggag
     4501 gttgctaaat ccatatactc atttttaaaa accccgtcac caatgacagt aagttcaaaa
     4561 tctattttct tatcaagtaa cgaaactgct ttgatcagta catcaatatt ctttcttttt
     4621 tcagtaagat ggctgacgca gatgagtctt agattactat tgtcaaaata tttttcggta
     4681 atagacacgt caaaattctg tccgagatga attactttaa tttcacgctc agttaatttt
     4741 tcattaacca actgcttctt attaagacca ctgtgaacaa ttattttatc ggcactttta
     4801 aaaacctgaa gaaactgaga tgctttatct ggaaaaagtt tttttaatgt tgggatatct
     4861 tcatgatgat ctatcacaaa tggaatatta agttctttgg taatgcctgc agttaccgga
     4921 gcccaggggc gaatccagtt acagtagatg agatcaggat tttgttcagt gataagtttt
     4981 tttagtttat tataaaaaaa gaaatttata aaagtattat cagcagattc aaaaaatggt
     5041 cttggcagag aaatgtatcc aaagtaaatt accctcaaat tgccttcact gtaatcagaa
     5101 gtatctttaa ttccgcttag ccagttatta aaatttttaa ataatccttc ggaatctcct
     5161 gtcaatattt cacgacctgg catatacctt agcggaacta tcataactac tttgcagtgt
     5221 tcggaaagag cttttgcctg tcgataaaca aattcacctg tgaaaggttt gtttttattt
     5281 gggaagcatt ctgatatttc gactatggtt ttcagatatt aatttctttt ttaagacaca
     5341 attttaactt catttctaat attcatttac tgctgacagc aacccaactc aaactctttc
     5401 cgcaggctgt gagggttttt ctgtggccgt ataaaataaa tcttacactt tatttcccca
     5461 tcctacatcc gcaaaattaa aatataaata tgaaaacttg aatctgagat agtaagaacc
     5521 ttcaccgctt gcgggcaggg cagggtgagg ttttcaactg tctaaagaat caagaatatt
     5581 ttttgttatg gtttttaaag tatatttttt cataacaagc tctcttgcct tctttgtcat
     5641 atctatcatt tcgttttcat tctttagaaa atattccgct ttgtctttaa gttctttttt
     5701 agatttaaaa catacgactt ctccatccgg aaaaaagtta tcaaaatcct ttttgtattc
     5761 tgataaaata aatatgcctg aagcaattcc gtcaaagatc cttgtagata cgccgtttat
     5821 tacacctgaa ttaactacta ccgggtagat ctttgcacag tttgaagcaa tgtttacttc
     5881 ctttgctgat aggggaatat tagtaaaatg tttttcgagt tttggaaaat aggggaacag
     5941 ctcataccat aatttatcac cgaatatttt catattgaaa tctgagagtt catttaaaac
     6001 agcagctctt tttattcccc atgcatttaa aaaataacct gtgccgacaa ataagatatc
     6061 ggtctggtat ttttgcagtt gatcatttga aggagtaaaa ggaaagaaag tattttcatc
     6121 tgtaccaacc ggagtaaaga aaattttatc atcactgata tcaagcattt tcaatccgtc
     6181 tatccatcct gaatccggag taataatcag gtctgcattc ataaccgtaa gtagaaaaga
     6241 tttctttgag ggatataaaa tgttcggatc atcacccagg aatacgatta ctttagtgcc
     6301 gttattttta atgtaatcga gagtgtccgg catgacgaga cagtcattat aaataaaaat
     6361 atgagacggc ttttcagatc tgtatttttt taagatctca tcattaatta ttttgatgaa
     6421 tttatttttt atagagttat caaatcttct gggaagtcga tgagaataga cattcagctt
     6481 tccccagtaa tccggataga aattatgaat attataataa ttaacatttt taccgtggaa
     6541 gcggagtgct tcagcgagat aaaattcgag cggtccgaga tcctgttctc cgagcaccca
     6601 gtaaaggtta tgctgcgtaa ttgtatccgg cttcttcagt tgagtcttcc tgtttactta
     6661 tgttatataa aatatttcct attgccatga gaatcgcacc ggtggcattg aaataaaaca
     6721 tatcaatact gaaaatgctc ataaaaatta tcataagcat aacggatgca agtgaaacga
     6781 aaagaatata atgttcctga ataaaaggtt tgatattttt ataatactga taaaagaaaa
     6841 ctgagaataa aaatattcct gacgttccga atattgtaac aatattggga aatccaagat
     6901 cattagtaac aagatttctg gaaatcgaag aaaaaccggt accgaacaaa ggatactctg
     6961 accagatact tatcatttga aaaagaatat attcacgacc gagaaatgaa gagtcagttt
     7021 tatcggcaag gtcatttatt gaatttactc tgagcagaaa aatgtcaaag atctgattaa
     7081 aggtagatga agtaaataaa agtatcacac caattaatgc gaaggatcca atgaaagcga
     7141 tcaatttgag acgtacattt tccctgctgt cttttttcag aaaaaagaat atggaaattg
     7201 tcaaaaggaa aaatgcgaat ccgatgatcc agatcctgct gagtgctatc ataatagaaa
     7261 gcagatatgc agttagcagc agaagtccgt aatttctttt ttctgagaag aagtaatagc
     7321 atccgaataa taaattgaac ggcaaaagat atccgatccg cgaccagata tatccggttt
     7381 taattcctcc gaattctaca gaaaaaggat cttcaccata aaacaaacta tcactttcaa
     7441 ataatctgaa aggaattctt ttttgagaaa tatattctgt aacctgtatt acaataaaaa
     7501 aaaatacaag aaacacgatc gttttttcaa aataattaaa ctgttttata tctctaattg
     7561 aaactaccag atatggcaaa aagagaaggt taccgaacgg tctgataaca ctgatcaaat
     7621 ctttcagaga aaaggaattg attaacagag gaatgacaaa agcaattgaa cataatataa
     7681 agaagatcaa agctgagagc ttgtaattgt aattcttcaa ttcactaatt ttcaataata
     7741 caacaataag caaagcaaga aaaaatacat ccataaaata tattgtacca attccaccga
     7801 agtctccgat ctcaagattt ggatttacat aaatgaattc accaaaattt gttataacca
     7861 tcagacaggt tacaataccc gacttgtaat tataaagaag aagtacaagt acagaggtca
     7921 gcagaaagta tattagtaag tttatcattt atgaaacatt cggttttata ttttccatat
     7981 aatcactgta tgtaattgcg agtacaatat catcataata ttttccgttt ctgaaaagtg
     8041 attcagggat tttcccggtg attctgaatc cggcccgttg agcagttttg agactggctc
     8101 cattatattc accaatcttt gcatgtagag tatgaatgtt aagagtttca aacagatatt
     8161 taaatatcat tctaaaacat tttaaactgt aaccttttcc tcggtaaact gaaaatattt
     8221 ccatacccat ttccgtattc ctgtttgtca tatcaagtcc ctgaagtttc cagactccga
     8281 ttacctgatc aggttcatct ttgaacatac aataaaccgt attatgttta ttcctggtaa
     8341 tattattaaa ccattcttcc tgattggtct cagttattat aaaaggatct ttaagcatta
     8401 acagaactga ttcgtcatta tgttctttac gaataagttc aatgtctttt ctttcaacgg
     8461 atctgaatcc caggtcgagg tattttatca tttacaaaat atttctaaaa aattatttgt
     8521 aaatataaat ttattatgta ggaaattata
//
LOCUS       DAORVQ010000144         8501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4711_length_8501_cov_6.106323, whole genome shotgun sequence.
ACCESSION   DAORVQ010000144 DAORVQ010000000
VERSION     DAORVQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_8501_cov_6.106323"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1276
                     /locus_tag="PLX80_10855"
     CDS             236..1276
                     /locus_tag="PLX80_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HQY21330.1"
                     /translation="MKKIFSVILFICLLTVINTVYPCTRVVYNGPNGTILTARSMDWE
                     TEIYTNLWIFPKGMDRNGEAGSNSLKWKSEYGSIVAAAFEIASSDGMNEKGLVANLLW
                     LAESQYPTFNSEKPGLSVAAWLQYVLDRFATVEEAVNELKEDKFTVVTSVIPGTEKIA
                     TVHLAISDPSGDNAIFEYIDGKLEIHHNKKYQVMTNSPAFEKQLALNEYWNDIGGTVM
                     LPGTNRAADRFIRASFYIKAIPQTDNIKDALASVFGVIRNCSVPLGITTPGQPNISST
                     LWRSVSDQKNLVYYFELTTTPNVFWVDLSSVDLSETGKVKTLKLTGGEIYAGNASDKF
                     IDSAPFKFAGLD"
     gene            1403..1525
                     /locus_tag="PLX80_10860"
     CDS             1403..1525
                     /locus_tag="PLX80_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21331.1"
                     /translation="MNPKLRLSGEFDYGKDSKNLEHLKILIQGNTRNAIEEKTG"
     gene            1677..2537
                     /locus_tag="PLX80_10865"
     CDS             1677..2537
                     /locus_tag="PLX80_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21332.1"
                     /translation="MAEILLSIIGISAIAAIPAQSVVLGLNTGKPYFKELLSQKSLLA
                     RFTITMFVIIPAVVILFSFITGNLINIWSAVLIISVAPASPGVIKNMRKLEGDQNVST
                     AWMILTMILSLIFFPLILLSLEKYLDIDLKLGISDVLIKLMLLFIFPMSIGFLISKYF
                     QGKAEILKKILIPLSKVAMITLIISLLISSVPMIISKGMIPVILLLSFIIVSLTIAHL
                     MGSPGNEFGPILPYSIILRLPAPAVILTKLNNTMEIHLPVLISYTILAAIVMMVYNKI
                     FFREKRVESI"
     gene            complement(2844..4157)
                     /locus_tag="PLX80_10870"
     CDS             complement(2844..4157)
                     /locus_tag="PLX80_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21333.1"
                     /translation="MRNMIFIFCCIFIFAGRIQSQENFEYPTGDSLYQHWGNYGPLVF
                     GPYKHIIISPPTLTFPLYSGSTLGNSVMLDTVDLEILRTFMNHFGFSSGNVYISFIAR
                     AGAAKPDGGRFINSGGSLIFNNNFMSEGGVYTKDSSGKIAFGIAKNNEQPVYTPAVYN
                     RATNYLIIYKYSFVDGGTSNDSLSLFVFSSACPSLEPAPLVSIKPSSPDFPDIGSVWL
                     YQGLNSNSPRIRVDGINMMSSWDNNVLPVELSSFNANVERSDVNLNWTVVSEINNSGF
                     DVERKSNGESNSWNKIGFVEGRGTTNEPMTYYYKDSKPESGRYQYRLKQSDFNGNFEY
                     FTLENDVVIGTPVSFTLNQNYPNPFNPVSKIDFAIPVNGLVTLKVYDILGKEVTVLIN
                     EVKEAGFYSVKFDGTNLSSGVYFYSINVDGGGQKFSKTLKLVLSK"
     gene            complement(4493..5200)
                     /locus_tag="PLX80_10875"
     CDS             complement(4493..5200)
                     /locus_tag="PLX80_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21334.1"
                     /translation="MKYKKSNIITVMLILTISLIVISSNGCSEDSVTTGTGSDNLELS
                     VFSSNESIGSGSILILDTVKILISDIKLNVSNNNEDSLNFKTGPFVLYLDLFSNVTFI
                     SSAIVSSGTYDKISFKVHKLNNNETVSDPEFSDPNGNYSVIVKGSYNGVRFVYRSTKS
                     AHQKITFPLNLDLESSGRSNLTLFVRPYLWFYGSNNVFLDPLDPSNSNEIDNNIKDNI
                     NNNFRCFVDNDKNGLPG"
     gene            complement(5242..5778)
                     /locus_tag="PLX80_10880"
     CDS             complement(5242..5778)
                     /locus_tag="PLX80_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21335.1"
                     /translation="MKRSFLILVFGLIYINTANGQLKDGDALLGPSLGFWSGSDAVTL
                     GLNYESQVTQMGDVASLGLGGVFRYSSFRSNNSSNDYYENNYVTLGFQTNMNFNRIAD
                     GKFVPMVGLVLGYNNVNSTRVKNNGNIDDKNYNSGLWLWGQLGARYFFTPKVAGVLRI
                     GAGNYNFNTVELAVDFKL"
     gene            complement(6376..8370)
                     /locus_tag="PLX80_10885"
     CDS             complement(6376..8370)
                     /locus_tag="PLX80_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY21336.1"
                     /translation="MKSFILAVIFLTITIETSVAQNVLISDRLAVKIKSLNPVEFTGV
                     LILLKDRIDIEMLDKELYEKNVSIEFRAETVINTLRKKAANTQGPLISYLNSEKSTGK
                     IKRMISYWITNMVFIEATPDVIDKLTKRNDIDIMDLDAELDIDRPYDERPAPNNTESI
                     ETGLRVVKADSLWKIGITGLGRTVMNIDGGVNGTHPALSSRWWGNNGRQWYHSWFDPI
                     SPFSTSPFDCATGGTYHGTHTMGIMCGRNSVSGDTVGVAPDARWMAAGITDCPGSSNP
                     SMNIAAYQWAMDPDTNSLTLDMPDVISCSWRDPSASNECTSSIYRTVLPAVEAAGIAV
                     IFSAGNSGPNASTVTPPKNINMDSVEVFCVGNVNGNTLVISNSSSRGPSLCGGSGTLL
                     IKPEVCAPGTSIRSTWSGTGYASISGTSMAAPHVAGCIALLKQAFPGMTGKQLKSVLF
                     TTATDLGAAGEDNIYGKGLVNVNRAYNYIKNSSAAVSNIKFIQEAYLNTGSGELNKND
                     TVRVYLRNNFAPYSIIDSGKAILDSVSFTAQITFLNTYTGTYFLQLKHRNSLETWSKA
                     GGESYTRGVAFNYDFTTTHTQAFGSNMILKGAKYCIYSGDVDQDGTVDLADGTLIDND
                     AFNFGSGYLQSDVNGDGLVDLSDAIYADNNAANFVSAVTP"
BASE COUNT         2763 a         1532 c         1353 g         2853 t
ORIGIN      
        1 tacattaaaa ttaaataaga cacttttccg cagttcccga atctgtttta taacttacct
       61 gatatttctt aataatacat tcagttaatg aattgagatt tccctgaact gatttcttta
      121 attatctgga aaacccattc atttttaatt tcaaaatttg ttttaaaaag ctaacttgta
      181 tttatgattc acaatctgtt aaataatctc atattaacct ttaaaaaata attttatgaa
      241 aaaaatcttt tccgttatac tttttatctg tttacttact gtaataaata cagtttatcc
      301 ctgcacaaga gttgtatata acggacctaa cggaactatt cttactgccc ggtctatgga
      361 ttgggaaact gaaatttata ctaatctctg gatatttcct aaaggtatgg acagaaatgg
      421 agaagcagga tcaaattctt tgaaatggaa atctgaatac ggaagtatag tagcagcagc
      481 atttgaaatt gcgagttcag acggtatgaa tgaaaaagga cttgtagcaa atcttctctg
      541 gctcgctgaa tctcaatatc caacatttaa ttcggaaaaa cccggattat ctgttgcagc
      601 ctggctgcag tatgtattag acaggtttgc cacagttgaa gaagcagtta atgaactcaa
      661 ggaagataag tttacagttg taacgtcagt tattcccggg acagaaaaaa tagctactgt
      721 acatttggca atatctgatc cttcaggtga taatgcaata tttgaataca tagacggcaa
      781 gctggaaatt catcataata aaaaatatca ggtaatgaca aactctccgg cttttgaaaa
      841 acaattagcc cttaatgaat actggaatga tataggcggg actgtaatgc tgcccggaac
      901 taaccgagct gccgacagat ttatcagggc atctttttat ataaaagcaa ttccgcagac
      961 agacaacata aaggatgcgc ttgcgagtgt tttcggtgtt ataagaaact gctcagttcc
     1021 acttggaata acaactcccg gccagccaaa tatttcttca acactatggc gatctgtttc
     1081 agatcagaaa aatcttgttt attattttga attaacgaca actccgaatg tattttgggt
     1141 agatctgagt tcggttgatc tttctgaaac aggaaaggta aagactttaa agctgaccgg
     1201 aggggaaata tatgcgggaa atgcatcaga taaattcatt gattctgcac cttttaaatt
     1261 tgccggttta gattaagaat tctaagataa aagtaatccg ggacgaagaa ttttttacag
     1321 acaatttatt actatatcaa atataaaact aaaaaaccac ttcattaaaa taaatttata
     1381 tcacggacaa tctaagaatc atgtgaatcc aaagttaaga ctatcaggag aatttgacta
     1441 tggaaaagat agtaaaaatc ttgaacatct gaagattctt attcaaggaa ataccagaaa
     1501 tgctattgaa gagaaaaccg gttaaagagt tattagtaaa aatacagcaa agaaattatt
     1561 gaaagacaag aaaaacattg aacctgaaaa tgaaatgact taaatatata aaattaatac
     1621 tatgtgattt gctttctaaa gaagctttta acttttaact tttaattttt aactctttgg
     1681 ctgagattct tttatctatc attggtatca gcgctattgc ggcaatacct gcacaatctg
     1741 tagttctcgg tttaaataca ggcaaacctt atttcaaaga attactgagt caaaaatctc
     1801 tactggcaag gtttacaata acgatgtttg taattatccc tgcggttgta attttgtttt
     1861 cattcattac cggaaatctt ataaatatct ggagtgcagt attaataatt tctgttgcac
     1921 ctgcttcacc gggagttatt aaaaacatga gaaagcttga aggtgatcaa aatgtcagca
     1981 ctgcatggat gatcctgaca atgatacttt cattgatttt ctttccgcta atacttttat
     2041 cactggagaa atatcttgat atagatctca aactcggaat ttccgatgtg ttaatcaaat
     2101 taatgttact ctttatattt ccaatgagta ttggattttt gatctcaaaa tattttcaag
     2161 gaaaagcaga aattttaaaa aagatattga ttcctctttc aaaggtggct atgattactc
     2221 ttataatttc acttctcatt tcctcagtcc caatgataat tagtaaaggt atgattccgg
     2281 ttattttatt gctgtcattt attatagttt ctcttacaat tgctcattta atggggtctc
     2341 caggaaatga atttggtcca atacttcctt attcaataat cctgagactg cctgctccgg
     2401 ctgttatact tacgaagtta aataatacta tggaaataca tttacctgtt ctcatttcct
     2461 ataccatact tgcggctata gtaatgatgg tttacaataa aatatttttc agggaaaaga
     2521 gagttgagtc aatataatat tttcattatg ccaaataatg gattgttgta aaaacaacac
     2581 agacccaaag tcacaaagta ttttctttat gtcttcgggt tctggttatt aaaaatatta
     2641 tttgaataaa tcctgcacaa gtttgctaaa cttctgccct ccatcttcaa catttccgtc
     2701 tctgcagagt cacgtgcttt tcggactctg cggaaaacca aaggtatatt gaaatacatt
     2761 tttccgattt aaaaaaacaa caaacaccca aagccacaaa gttttacctt tgtgtctttg
     2821 tgccttcgtg gtaaaaaaaa tctttatttc gacagcacaa gtttcagcgt cttactgaat
     2881 ttctgaccgc cgccgtcaac attaatactg taaaaataaa ctcccgaact taaattcgtc
     2941 ccgtcaaatt tcacagaata aaatcccgct tctttgactt cattgataag tactgttact
     3001 tcttttccaa gaatgtcata tactttcaaa gtaaccagtc catttacagg aattgcaaag
     3061 tctatttttg agactggatt aaatggattt ggataattct gattaagtgt aaaactaaca
     3121 ggtgttccga tcacaacgtc attctcaagt gtaaagtatt caaaatttcc attaaagtcg
     3181 gactgcttca gacgatactg atatctgcct gattcaggtt ttgagtcttt gtaatagtaa
     3241 gtcatcggct cattagtcgt acctctgcct tcgacaaagc ctattttatt ccatgaatta
     3301 ctttctccgt tagattttct ttcaacatca aatcccgagt tattgatttc agaaacaacc
     3361 gtccagttca aattcacatc agatctttct acatttgcat tgaaggatga taactctaca
     3421 ggaagcacat tattatccca ggagctcatc atatttattc cgtcaactct gatccttgga
     3481 gagttagaat tcagaccctg atataaccag acagatccaa tatccggaaa gtcagggctt
     3541 gaaggtttta tacttacaag cggagccggt tctaatgaag gacatgcaga tgaaaaaaca
     3601 aaaagactta aggaatcatt acttgttcca ccatcgacaa aactgtattt gtaaattatt
     3661 aaataattgg tagctctgtt gtaaactgcc ggtgtataaa ccggttgttc attatttttt
     3721 gcaattccga atgcaatttt ccctgatgaa tctttcgtat aaactcctcc ttctgacata
     3781 aaattattat tgaaaatcaa agatccgcct gagttgataa atcttccacc atccggtttt
     3841 gctgcacctg ctctagctat gaaagaaatg taaacatttc ctgatgaaaa tccgaagtga
     3901 ttcataaatg tccttagaat ttctaaatcc actgtatcca gcataacaga atttccaaga
     3961 gttgaccctg aatataaagg aaatgtaagt gtcggaggag aaataattat atgtttgtaa
     4021 ggtccgaaaa ccagtggacc gtagtttccc cagtgctgat acagggaatc tcccgttgga
     4081 tattcaaaat tttcctgtga ctgaattctg cctgcaaaaa tgaaaatgca gcagaatatg
     4141 aaaatcatgt ttctcataaa cttaatttta tttctgaaag ttaatttatt taaactgaat
     4201 tttaaagact ttggaacatg catttttgaa atgaagaact aaatgaaaaa tgtaaaagag
     4261 gtgcccggag tcaggtggta aaaatctaat aagagtttat taatattgag attatttaac
     4321 tcaaacaatt tgtttcctaa ttagatttta tgaattcaat accaagcaaa agtcacaaag
     4381 tttttctttg tttcttcgga acaaagcgtc aaaaaaaaac ctgactttaa atttgtatcg
     4441 tcaaatttta caagtcaggt tttttaataa atcaattttc ttcacatttc atttaaccgg
     4501 gcaatccatt cttatcatta tctacaaaac atctgaaatt attattgata ttatctttta
     4561 tattattatc aatttcattt gaatttgacg gatccagcgg atcgagaaag acattattac
     4621 ttccataaaa ccaaagataa ggtcttacaa atagtgtaag attagatctt cctgaggatt
     4681 ccagatccag attaagcggg aaagtaattt tctgatgagc agatttagtt gatctgtaaa
     4741 caaatctgac tccgttataa cttcctttta ctattactga atagtttccg tttggatcag
     4801 aaaactccgg atcagacaca gtttcattat tgttaagttt atgaactttg aaactgatct
     4861 tgtcatatgt tcccgaagat actattgctg aactaataaa agtgacattt gaaaacagat
     4921 ccagataaag tacaaaagga ccggttttaa aatttaagga atcctcattg ttatttgaga
     4981 catttaattt aatatcgctg ataagtattt tgacagtatc caaaatcaat atgcttccac
     5041 ttccaataga ttcattactg ctgaatacac ttaattcaag gttgtctgat ccggtaccag
     5101 tggttactga atcttcacta cagccatttg aacttattac tattaagctg atagtcagaa
     5161 tcaacattac agttattata ttactttttt tgtatttcat taatttacct ttctgagaga
     5221 ttctcatttt tagaatttat tttaaagttt aaagtcgact gccagttcga cggtattgaa
     5281 gttataattc cctgcgccga ttctcaacac acctgccacc ttgggagtaa agaaatatct
     5341 tgcaccaagc tgtccccaaa gccatagacc gctgttataa tttttgtcat cgatatttcc
     5401 attatttttt accctggtgg aattaacatt attatatccc agcaccagac caaccatcgg
     5461 cacgaatttt ccgtcagcta tccggttaaa attcatgttc gtctgaaaac caagagttac
     5521 ataattattt tcataataat cattactgga attattactt ctaaatgatg aatatctgaa
     5581 aacccctccg agtccgagag atgcaacatc acccatctga gtgacctgac tttcgtaatt
     5641 taaacccaaa gttactgcat cagatcccga ccagaatcct aatgacggtc cgagtaatgc
     5701 gtccccgtcc tttaactgtc cgtttgctgt gttgatataa attaaaccaa atactaatat
     5761 taaaaatgac cttttcattt taataatcct ttctgatagt taaacatatt agtactatcg
     5821 caaatcctgt gccatatgct taaaaaacaa aaaaatacat ttattgagta tcagatgaca
     5881 atatttgaca taatgtgtca gagaggattg ttgatattag tcttaatttt tttaaaactg
     5941 aagctgcgca aatatcatta ttctttaaaa acaggacgat gctcagtcca ataagatatc
     6001 cctgctcaga atcacatata aattttttat aaagagcgta ttttcatact tttgaggcag
     6061 tctcttttaa gggggaatta cttttaaatt attctgaatc gtactttttg aaatttatac
     6121 ttcagttcta aaaaattgat taaaaatttt cttaccattt tgcgattaat ttacattgta
     6181 aagaattaat tcgtaaatcg taataaatta attcgtaatt cgtaattcgt aaatcgtaat
     6241 aaattaattc gtaaatcgta ataaattaat tcgtaattcg taaatcgtaa taaattaatt
     6301 cgtaattcgt aattcgtaaa tcgtaataaa ttaattcgta attcgtaatt cgtaattcgt
     6361 aattcttaat tgctcttaag gcgttactgc acttacaaaa ttcgctgcat tattatctgc
     6421 ataaattgca tcagacagat ctactaatcc gtcaccgttt acatcggatt gtaaatatcc
     6481 cgatccaaaa ttgaatgcat cattatcaat taaagtcccg tcagcaagat caacagttcc
     6541 gtcctgatct acatcgccgc tgtaaataca atactttgca ccttttaaga tcatgttact
     6601 tccgaatgct tgagtatgcg tagttgtaaa atcataattg aatgcaactc ctcttgtata
     6661 agactcacca cctgctttac tccatgtttc taatgaattt ctgtgcttta gctgtaagaa
     6721 atatgtgcct gtataagtat ttaaaaaagt tatctgagct gtaaaagaaa ctgaatctaa
     6781 aattgctttt cccgaatcga taattgaata tggagcaaaa ttatttctga gataaactct
     6841 tacggtatca tttttattga gctcacctga gcctgtattc agatatgctt cctgaataaa
     6901 ttttatattg gaaactgctg cagaagaatt tttaatataa ttgtatgccc tgttcacatt
     6961 cactaaacct tttccatata tattatcttc acctgctgca ccaagatcag ttgctgtagt
     7021 aaataatact gactttaatt gttttccggt catacccgga aaagcctgtt tcaaaagtgc
     7081 aatacagccg gctacatgtg gggcagccat tgaagttccg ctgatcgaag cataaccggt
     7141 acctgaccat gtggatctta ttgaagttcc gggggcgcaa acctcaggtt taataagaag
     7201 tgttcccgat ccgccgcaca gagatggacc tctgcttgaa gaattgctga tgaccagtgt
     7261 atttccgttt acatttccca cacaaaaaac ttcaactgaa tccatgttga tattttttgg
     7321 aggtgtaaca gtagaagcat ttggtccgga atttcctgct gaaaaaatta ctgctatacc
     7381 agctgcttcg actgctggaa gtacagttct gtaaattgaa gaagtacatt cattacttgc
     7441 acttggatct ctccacgaac aactgatcac atcaggcatg tccaatgtaa gagaatttgt
     7501 atcgggatcc atcgcccact gataagcggc aatattcatt gaaggattag aggaaccagg
     7561 gcagtctgta attcctgctg ccatccatct tgcatcagga gctactccga ctgtatcgcc
     7621 acttacagaa tttcttccgc acataattcc catagtatga gttccatgat aagtaccgcc
     7681 ggttgcacag tcaaaaggcg atgtactgaa tggtgagatc ggatcaaacc atgaatgata
     7741 ccattgtctt ccgttatttc cccaccatct tgaagaaagc gccggatgtg ttccgttgac
     7801 accgccgtca atgttcatca ctgtccttcc caaacccgtt atcccaatct tccataatga
     7861 atctgctttt acaactctta gtcctgtctc tatggattct gtattattcg gagcaggtct
     7921 ttcatcataa ggtctgtcaa tgtcaagctc tgcatcaaga tccattatat caatatcatt
     7981 tctctttgtt aatttatcga tcacgtcagg tgtagcttcg ataaatacca tattagtgat
     8041 ccagtagctg atcattcttt ttatcttacc ggtacttttt tctgaattca aatacgagat
     8101 caaaggaccc tgtgtatttg ctgccttttt gcgtaaagtg tttattactg tttcagctct
     8161 gaattcgatt gatacatttt tttcataaag ttctttatca agcatttcaa tatcaattct
     8221 gtcctttagt aaaataagaa cacctgtaaa ttccacagga tttaatgact taattttaac
     8281 agcaagtctg tctgagatca ggacgttttg agcaactgaa gtttctatag ttatagttaa
     8341 aaagattaca gctaatatga aagatttcat ttagttaatt gttaatttta taaaattttt
     8401 taatgaaaga atatattgct ttagataatt atattcaaag taatattgag attagaagga
     8461 aatattgatt ttttcttaat ccaaatttat ttataaaggc t
//
LOCUS       DAORVQ010000145         8333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4868_length_8333_cov_4.577555, whole genome shotgun sequence.
ACCESSION   DAORVQ010000145 DAORVQ010000000
VERSION     DAORVQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4868_length_8333_cov_4.577555"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLX80_10890"
     CDS             <1..560
                     /locus_tag="PLX80_10890"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21337.1"
                     /translation="MEQLVDKFSLEKVQSSGAVFNIDKLNWMNNSYIKDYDPDKLFEL
                     SIPYFKDTGVDLADKEHAKKILLAIRVYLNKLDEIPDHTKIFTQDKIVIEDPELEKIL
                     TADTSVTVFKELTDQFKNIDELTAEVYKEKISEVQNVTGVKGKLLFKPVRVALTGSEN
                     GPDLPSIVEILGKEKVVRFLTDWIR"
     gene            complement(759..1046)
                     /locus_tag="PLX80_10895"
     CDS             complement(759..1046)
                     /locus_tag="PLX80_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY21338.1"
                     /translation="MTRSQIAAAISGATGLSKKETEIIVEGFISCVIDSLKRNESVEI
                     RGFGTFKNNMKHPRIARNPKTGEKISLGKRYIPMFKVSKDFKKVVQESLVK"
     gene            complement(1065..2018)
                     /gene="sppA"
                     /locus_tag="PLX80_10900"
     CDS             complement(1065..2018)
                     /gene="sppA"
                     /locus_tag="PLX80_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQY21339.1"
                     /translation="MENENNQPVKSNSGRWFWGIFISLIVIAMFFVGISFLYLTKIIS
                     GSDGSLEINGKGNGKIAEVKLDFTIFSSEGIVRQFKKYGEDKSIKAIILRINTPGGGV
                     AASQEIYEIVKKTRDSGKPVIVSIASLGASGGYYAACGGSTIIADEGSLVGSIGVIIQ
                     LVNYKELADKIGIKSMTITSGELKDAGNPFKDFSEKDRTYFQEIVDDTFEQFLGVVSK
                     ERKIDTAELKQYATGRVFTGRQALKIGLIDSIGTYEDAIRIAANMAGIDGEPTIVKEK
                     EKSTLIERILDTFSNNELRSIKEELRSEFIDQPLLQYKFVY"
     gene            complement(2030..2974)
                     /locus_tag="PLX80_10905"
     CDS             complement(2030..2974)
                     /locus_tag="PLX80_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21340.1"
                     /translation="MKSNKLSLFVLPLIFVLAIGAIYFQGCSSAQSTTGKLAFSQQDY
                     LKAEEELKKGLAIDKGDAEGWYMLGYSQVENGNYAEAQKSFAQSLTISNAFSSSIQSF
                     WVDKFNAGAKDFKNGIDAEEKKNSASAKTFYESALRNFEASSVIFPDSLKSYSAIGET
                     YLALGNTEKALEVFNDLAAKSTNQEDAERVAKILFDSGLNMMSLNNFKAASETFKKVL
                     AIPNLPKTNEFYETSAYNNGLALAKLGEDARVKDENSNYKEYFNDALLSLEPLTYSLS
                     KKELEKQVYELLVSVYANLGRTEEAQDALNKKNAILGN"
     gene            complement(3073..3993)
                     /gene="xerD"
                     /locus_tag="PLX80_10910"
     CDS             complement(3073..3993)
                     /gene="xerD"
                     /locus_tag="PLX80_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQY21341.1"
                     /translation="MNTDIEFHKKLFLNFLIIEKSLSENTLFSYRSDLERFIKYLNEI
                     KKISNVKEIDDNIINQFLKHLNKHGDKNEKKYSDKSVARFISTLKSFFKYLESEDVIS
                     INPVEELESPRTSRTLPEVLTIEETELLLSMPDENDKLGLRDKALLETMYASGLRVSE
                     TSDLETGNIFFDEGYLRVIGKGSKERIVPIGDIALNFIVKYINESRSLLKKSKSGNII
                     FLNFRGGKLSRMGILNIINKYSNLAGIKKNVHPHTLRHSFATHLLKGGADIRIIQEML
                     GHSDISTTQIYTHIDREYLIEVHKTFHPRA"
     gene            complement(4000..6279)
                     /locus_tag="PLX80_10915"
     CDS             complement(4000..6279)
                     /locus_tag="PLX80_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQY21342.1"
                     /translation="MNLKDKFSGGEFLKQFSRIDLKTSSLIKLFIAIITVTALFLMIP
                     SYKNIESKYEVGAIWSSEDLIAPFSFPVYKDESEYDQEKKDVIKNIALVFDKDDSEKN
                     ISDSLENLRREMTEVFEITDEIIKNNEGFINSEKLDVHKAKIGIDFTFAEWEQFYSIY
                     KNLKNSDENKLNISFPEYLNIVSSVVLKNSDGDIINLDKNNITSKKITVQHKNDKLQE
                     LSDLDKVKDKNEILFKYESDFKNKFSDSLLVSSALKISNKFTSENLIYNNELTEKETQ
                     SRIEQIPKTIGIVKENERIISKHDPVNRFTKMKLDSYKKVRLEKIGTQDVILQYVGKF
                     FTVIILLIVLGFFLYYIRPAVFNDNFKLMIISSMILIEGFIAFLSMSLDLRLPIELLI
                     FVPVASILLTIIFDSRLAFYTVTIICFFVATIRGGDFSIAFITFCGSVLAIFSERDIK
                     NRSQIFRSFFFILIGYTISILAVNLIRIEDYQKLWVKLMFGGINAVMSPVIAFGLLIF
                     YEKVFKITTDLTLLELSDFNHPLLKELSSKAPGTFHHSIIMGNLSEAAAEAIGANQIL
                     ARVGCYYHDIGKIMKPQYFIENQLDEYNKHNELNPTVSTKIIIAHVTDGIALAKKYKL
                     PQEIIDFIPMHHGTTLVSYFYDKAKKSTKEELSDYIYRYPGPKPQTKETGIVMLADAI
                     EASTRAIEDPTPQKLESKIREVIKVRFMEGEFDECDLTLRDLTHIKDSFMKVLIGIHH
                     HRIKYPDKNQLELVSNSSE"
     gene            complement(6365..6664)
                     /locus_tag="PLX80_10920"
     CDS             complement(6365..6664)
                     /locus_tag="PLX80_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21343.1"
                     /translation="MPKIIFEINYSIHPEKRDAYLETIKEIKNNINGSMDSNYSVFES
                     KKNPNNFTEIFHCESEDEIDSIEDNQSEETKELISKLFDEYIQDKKVLYSTKYEV"
     gene            6892..>8333
                     /locus_tag="PLX80_10925"
     CDS             6892..>8333
                     /locus_tag="PLX80_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21344.1"
                     /translation="MTKYFLPVLLFLFLYSNPVKSEAQSDTQILEGLDLIYNLEFDDA
                     EILFKSYQKNNPDDLKGYFYESLIYFYKALPSRDEKLYGKFLELSNNVISKAEKKLDV
                     NKNDVEALYYKGIAHSYISLLMLSLNKSLLEAASNGNDGYRILTAVIENYPEYYDAYM
                     GLGLYKVALGFVPDKFKWLLSLIGFDGNIKDGVRFLNVSREKGIYTKVDSKVFLSIFS
                     LKEEEEQDQTALNYSEDLTEEYPQSPVFRVFYSSLLLQFGYNEKAIENANKSLELNTR
                     SFKSEIEKSSFAILGTAYFRLNDYDKSIQFLEKYMTYVNPEDRYNVYLFTLGAAYELS
                     GNRSAAINKYSEVRNNFIEERDGELDKIFYRYSREKLKVPMNTLDSLLILALNLRESL
                     KFEESVNIYEEISEKSLLQKYNTVDYKIKFHFESGLSYSYAGNTKKALEQFNKCITLS
                     PENETWLVPHSYFEMGKIYFREGKKDKAEE"
BASE COUNT         2689 a         1369 c         1226 g         3049 t
ORIGIN      
        1 ctatggagca gctcgtagat aaattttcat tagagaaagt acagtcttca ggagcagtat
       61 tcaatattga taaacttaac tggatgaata attcttatat aaaagattat gatccggata
      121 aattatttga actatctatc ccctatttta aagatacagg tgtggactta gcagataaag
      181 aacatgcgaa aaaaatattg ttagctataa gagtatattt gaataagctt gatgaaattc
      241 ctgatcatac aaaaatattt acacaggata aaattgtaat tgaagatcct gagctggaaa
      301 aaattctaac tgctgatact tcagttacag tttttaaaga gttgacagat cagttcaaaa
      361 atattgatga acttacagct gaagtttata aagaaaagat aagtgaagta cagaatgtaa
      421 caggtgtaaa aggaaagtta ttgtttaaac ctgtcagagt tgctttgaca ggaagcgaga
      481 acggacctga tctgccttct atagtcgaaa tccttggtaa agaaaaagta gtaagatttt
      541 taacggattg gatcagataa aaaatgccgt cagataaatt ctgacggcat gaaaaaaact
      601 ttatatctat ctaattttct ttttgtgtct gttttttcta agtctttttt tccttttgtg
      661 cgtcgccatt ttatgacgtt ttcttttttt tccgcaaggc atagttaatt tattcctttc
      721 ttgtgtatag ttgttaaata aaaattctaa ttaaaatatt attttacaag tgattcctga
      781 acaactttct taaaatcttt agatacttta aacattggaa tatatctttt tcctaatgaa
      841 attttctcgc ctgtctttgg atttcttgca atcctcggat gtttcatgtt attcttaaac
      901 gttccaaaac ctcttatttc aacagattca tttcgtttta aagaatcaat aacacaacta
      961 ataaatcctt caacaataat ttcagtttcc tttttactta atcccgtagc tcctgatata
     1021 gcagcagcga tctgagatct ggtcataatc tctccttaat acagttaata aacaaattta
     1081 tattgtaaaa gcggctgatc tataaattca cttcttaatt cttcttttat agatcttaac
     1141 tcattattag aaaatgtatc aagtattctt tcaataagag ttgatttttc tttttctttt
     1201 acgatagtag gttcaccatc aattcctgcc atatttgctg ctattctgat agcatcttca
     1261 taagtaccaa tcgaatctat aagtccgatc tttaatgcct gtctgccggt aaacactctg
     1321 ccggttgcat actgcttgag ttctgctgta tcgatctttc tttcttttga tacaactcca
     1381 aggaattgtt caaatgtatc atctactatt tcctgaaaat aagttctgtc cttttctgaa
     1441 aaatccttaa atggatttcc agcgtctttg agttctccac tggtaattgt catagatttt
     1501 ataccaatct tatctgcaag ttctttataa tttacaagct gaataattac tcctatactt
     1561 ccaacgagtg aaccttcatc ggcaataatt gtacttcctc cgcaggcagc gtaataacct
     1621 ccacttgcac caagacttgc aatagataca attactggtt tacctgaatc tctggttttt
     1681 tttacaattt cataaatttc ctgagatgca gcaactccac ctcctggagt gtttattcta
     1741 agaatgattg cttttattga tttgtcttca ccgtattttt tgaactgtct gacaattcct
     1801 tcagacgaaa aaattgtaaa atctagtttt acttcagcga tttttccatt tcctttacca
     1861 ttgatctcaa gtgatccgtc agaacctgaa attatttttg taagatataa aaatgaaatg
     1921 ccaacgaaaa acattgcaat tacaatcagt gaaataaata ttccccaaaa ccatcttccg
     1981 gaattagatt tcacaggttg attattttcg ttttccatta tttttattat taattgccta
     2041 aaattgcatt tttcttattc aatgcatcct gagcttcttc agttctgcca agatttgcat
     2101 ataccgaaac taataattca tatacttgct tttcaagttc ttttttggac agactataag
     2161 taagtggttc taaagataat aaagcatcat taaaatattc tttataatta ctgttctcat
     2221 cttttactct tgcgtcttct cccaatttag caagtgccag tccattatta taagcagatg
     2281 tttcataaaa ctcattagtt tttggtaaat ttggaatagc taaaactttt ttgaaagttt
     2341 cacttgctgc cttaaaatta tttaatgaca tcatatttaa acctgaatca aaaagtattt
     2401 ttgctactct ttctgcatct tcttgattag tagatttagc agctaaatca ttaaaaacct
     2461 cgagtgcttt ttctgtgttc cctaatgcaa gataagtttc accaattgca gaataagact
     2521 tcaaactgtc cgggaaaatt actgaagatg cttcaaaatt tctgagtgca ctttcataaa
     2581 aagtttttgc actggctgaa ttcttttttt cttcggcatc aattccattt ttaaaatcct
     2641 tagcacctgc attaaactta tcaacccaga atgactgaat tgaactgctg aaagcatttg
     2701 aaattgttag actttgagca aatgatttct gagcttctgc ataatttcca ttttctacct
     2761 gtgaatagcc gagcatatac cagccttcag cgtcaccttt atctattgcc aaaccttttt
     2821 ttaattcttc ttcagctttc aaataatcct gctgagaaaa agctaatttt cctgttgtag
     2881 attgtgcaga tgagcaacct tgaaaataaa ttgctcctat tgctaaaaca aaaataagtg
     2941 gtaatacaaa taaagataat ttatttgatt tcattataaa atttctcctg atgatttaaa
     3001 ttttttagta ctgtaaatat acggattata tgacatttat tcaatttaac aatattactc
     3061 tctttttaat ttttatgctc ttggatgaaa tgtcttatgt acttcaatca gatattccct
     3121 gtcaatatgt gtgtagatct gcgttgtaga aatatccgag tgtccgagca tttcctgaat
     3181 tatcctgatg tctgctcctc cttttaatag atgcgttgca aatgaatgtc ttagtgtatg
     3241 aggatgaaca ttttttttta tacctgcgag atttgaatac ttgttaatga tattcagaat
     3301 tcccatcctc gacagctttc ctcctctgaa atttaaaaat attatattgc cggatttact
     3361 ttttttcaaa agagatctgc tttcgttaat atatttcaca ataaaattta atgcgatatc
     3421 acctatcgga acgatccttt cctttgaacc tttaccaata actctcagat aaccttcatc
     3481 aaaaaaaata ttccctgttt caagatcaga tgtttcacta actctaagtc cgctggcata
     3541 cattgtttct aataatgctt tatccctgag tccaagttta tcattttcat caggcataga
     3601 aagaagcagt tctgtttctt ctatcgtcag aacttcaggc aatgtcctcg aagtccttgg
     3661 cgactcaagc tcttctacag gatttatgga aatgacatct tcagattcaa gatatttaaa
     3721 aaatgatttt aaggttgaaa tgaatcttgc tactgattta tctgaatatt ttttttcatt
     3781 tttatctcca tgtttattca gatgtttaag aaattgattt ataatattat catcaatttc
     3841 ttttacattg ctgatctttt ttatttcatt aagatatttt ataaacctct cgaggtcaga
     3901 tctgtaggaa aatagggtgt tttcggataa tgatttttct ataattagaa aattcagaaa
     3961 tagtttttta tgaaattcaa tatcagtatt cataaatttt tattcggaag aattagaaac
     4021 cagctcaagc tgatttttat cgggatattt aattctgtga tgatgaatac caatgagtac
     4081 tttcataaag ctatccttta tatgtgtaag gtctctcaat gtaagatcac attcatcaaa
     4141 ttcaccttcc ataaatctga ctttaataac ttcacgtatc ttactttcga gtttctgcgg
     4201 cgtcggatct tcaatcgctc ttgtgctggc ttcaatagca tccgcaagca taacgatacc
     4261 ggtttctttt gtctgtggtt ttggacctgg atatctgtaa atgtaatcag ataattcttc
     4321 tttggttgac ttttttgctt tgtcataaaa ataagaaaca agtgtagtcc cgtgatgcat
     4381 tggaataaaa tctattatct cctgaggcaa tttgtatttt tttgcaagtg caattccgtc
     4441 agttacatgc gctattatta tcttagtgct gactgtcgga ttgagttcat tgtgtttatt
     4501 atattcatca agttgattct caataaaata ctgcggtttc ataatttttc caatgtcatg
     4561 ataataacat cctaccctag ccagtatctg atttgcgccg atagcttcag cagcagcttc
     4621 ggataaattt cccattataa tgctgtgatg aaatgtaccg ggtgcttttg aagaaagttc
     4681 ttttaataaa ggatgattaa agtctgacaa ttcaagcagc gtcagatctg tagtaatttt
     4741 aaaaactttt tcataaaaaa taagaagacc gaatgcaatg actggagaca ttactgcatt
     4801 tattccgccg aacattaatt tgacccagag tttttgataa tcttctatcc gaatcagatt
     4861 tactgcaagt attgaaattg tgtatccgat cagaataaaa aagaatgaac ggaatatctg
     4921 tgatctgttt ttaatatccc tttcgctgaa gattgccaat accgatccgc agaatgtgat
     4981 aaaagcaatt gagaaatctc cgccacgtat tgttgcaaca aagaaacaaa ttatagtcac
     5041 agtataaaac gcaagtctcg agtcaaaaat tattgtaaga agtatagatg ctaccggtac
     5101 aaatattagt aattctattg gcagtctcag atctaagctc atactgagga atgcgataaa
     5161 tccttcaata agtatcatcg atgaaataat catgagcttg aaattgtcat taaagacagc
     5221 cgggcggata taataaagaa aaaatccaag tactatcaga agtattatta ctgtaaaaaa
     5281 ttttcccaca tactgaagaa tgacatcctg agttccaatc ttttcaagtc tcactttctt
     5341 ataagaatcg agtttcattt ttgtgaacct gttgaccgga tcatgcttgc tgataattcg
     5401 ctcattttct tttacaatcc cgattgtttt tggtatttgt tctatacggc tctgagtttc
     5461 cttttcagta agttcattat tatagataag attttctgaa gtaaatttat tgctgatctt
     5521 taatgcagag gacacaagca gggagtcact gaatttattt ttaaaatcag attcgtattt
     5581 aaataaaatt tcatttttat ccttcacttt atcgagatct gaaagctcct gaagcttatc
     5641 attcttatgc tgtacagtaa tcttttttga tgtgatatta tttttatcga gattaataat
     5701 atcaccatct gaatttttta aaacgacact tgaaacaata ttcaagtatt cgggaaatga
     5761 aatatttaat ttattttcat cagaattttt taaattttta taaatagaat aaaactgttc
     5821 ccattctgca aaagtaaaat ctattccgat cttagcttta tgtacatcaa gcttctctga
     5881 attaataaat ccttcattat ttttaatgat ctcatctgta atttcaaata cttcagtcat
     5941 ttcccttctt agattttcaa gactgtctga aatatttttt tctgaatcat ctttgtcaaa
     6001 tactaaagca atatttttta taacatcttt tttctcctga tcatattcag attcatcttt
     6061 atatacagga aatgaaaaag gagctatcag gtcttccgat gaccatatag ctcccacttc
     6121 gtatttggat tcaatattct tatagcttgg tatcatgaga aaaagagctg taacagtaat
     6181 tatggcaata aacagtttga taagactgct ggttttcaga tcaattctgc tgaactgttt
     6241 taaaaattca cctccgctaa atttgtcttt taaattcatg atgcttttgg gtaaaaagga
     6301 aagagtagtt ttatagacta ctctgttgta aatgtaagat ataaaaaata atcaggaata
     6361 aagttcaaac ttcatattta gtagaataca aaactttctt atcctgaata tattcatcaa
     6421 ataatttgct gatcaattct tttgtctctt cactctgatt atcttcaatg gaatctattt
     6481 catcttcact ttcacaatga aaaatttcag taaaattatt cggatttttt ttactttcaa
     6541 aaacagaata attactgtcc atagatccgt tgatgttgtt ttttatttct ttaatcgttt
     6601 ctaaatacgc atctctcttt tcaggatgaa tgctgtaatt tatttcaaaa atgattttag
     6661 gcatatttgt ttttaattat cataatatgc aaatataaaa acacatatat ctaaaataaa
     6721 gataaaattt ataataactt ttgtttctat tcgaacagtt gtataatgca tactgcattt
     6781 caaagaattc tcaaaaatat tattttgcaa aagtttattt caatcagtaa atttttcaat
     6841 ttctggaacg gtttcagaaa gttgtaagtt ctgtccaata ttaattttat aatgacaaaa
     6901 tattttttac ctgttttact ttttctattt ctatattcaa atcctgttaa gtccgaagct
     6961 cagtctgata cacaaattct cgaaggtctc gatctgatct acaatctaga atttgatgat
     7021 gccgaaattc tgtttaagtc ataccaaaaa aataatcccg acgatttgaa aggttacttt
     7081 tatgaatctc tgatctattt ttataaagct ctgccttccc gtgatgaaaa attgtatgga
     7141 aaattcttag aactctcaaa taacgtcatt tctaaagccg agaaaaaact tgatgtaaat
     7201 aaaaatgatg ttgaagcttt atattacaaa ggcattgcac acagctatat ttcacttttg
     7261 atgcttagct taaataaaag tcttcttgaa gctgcttcaa atggaaatga cggatacaga
     7321 attctcacag cagttatcga gaattatccg gaatattatg atgcctacat gggactcgga
     7381 ctttataaag tagcgctggg atttgttcct gataaattca aatggctttt atctcttatt
     7441 gggtttgacg gaaatattaa agatggagtt cgatttttaa atgtatcgag agaaaaaggt
     7501 atttatacaa aagttgattc caaagtcttt ctgtcgattt tctccctaaa ggaagaagag
     7561 gaacaggatc agactgcatt aaattattcc gaagatctga ctgaagaata tccgcaaagt
     7621 ccggtattca gagtgtttta ttcaagtctt ctgcttcagt ttggatataa tgaaaaggca
     7681 attgaaaatg ccaataaatc tcttgagctt aatacaagat catttaaaag tgaaattgaa
     7741 aaatcttctt ttgcaattct cggtacagct tatttcagac tgaatgatta tgataagagt
     7801 attcagtttc ttgaaaaata tatgacgtat gtaaatcctg aagacagata taatgtatat
     7861 ttgtttacac taggtgctgc ttacgaactt tcaggaaaca gatcagcagc tataaataaa
     7921 tattcagaag taagaaataa tttcatagaa gaacgtgacg gtgaacttga taaaattttt
     7981 tacagatatt cccgtgaaaa attaaaagtg cctatgaata ctttagattc acttttgata
     8041 ttagctttaa atttgaggga gtcattgaaa tttgaagaat cagtaaatat ttacgaagaa
     8101 atttctgaga agagtcttct gcagaaatat aatactgtcg attataaaat aaaatttcac
     8161 tttgaatccg gactttcata ttcatatgca ggaaatacaa aaaaagctct tgagcaattt
     8221 aataaatgta ttactctgtc acccgaaaat gaaacatggc ttgtcccaca ttcttatttt
     8281 gaaatgggaa aaatttattt cagagaaggg aaaaaagata aagctgaaga aat
//
LOCUS       DAORVQ010000146         8318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4882_length_8318_cov_4.994433, whole genome shotgun sequence.
ACCESSION   DAORVQ010000146 DAORVQ010000000
VERSION     DAORVQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_8318_cov_4.994433"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1122
                     /gene="rfbA"
                     /locus_tag="PLX80_10930"
     CDS             259..1122
                     /gene="rfbA"
                     /locus_tag="PLX80_10930"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQY21345.1"
                     /translation="MKGIILAGGAGSRLYPVSKVYSKQLTLIYDKPLIYYPLSILMLS
                     NIKEILIISNEETIPLYQKLFEDGADYGIKIEYALQTAPNGIAESFIIGEKFIGNDGA
                     TLILGDNIFYGNLDFIYRAMQKNDGATIFGYRVNDPERYGIVEFDNTGKAISIEEKPD
                     NPKSNFAVPGLYVYDNEVVEISKNIKPSARGELEITDVNNVYLQKGKLNVEKIGRGVA
                     WLDTGTPESLLQASNFFGVIEDRQGLKVACIEEIAYHKGFINKDEFERSISKIPNSLY
                     KTYLEKVLKEY"
     gene            1132..1677
                     /gene="rfbC"
                     /locus_tag="PLX80_10935"
     CDS             1132..1677
                     /gene="rfbC"
                     /locus_tag="PLX80_10935"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQY21346.1"
                     /translation="MNIIKTEVDGVLIIEPDFFPDARGFFFESYNKKNYNEAGINAEF
                     VQDNISKSVKGTIRGLHYQVGEFAQGKLCQVISGSVLDVAVDIRSGSPTYGKHVSIEL
                     TGKNHKQIWIPAGFAHGFSVLSDEAIFSYKCTALYSKKDERSILYNDPALNIDWKLSE
                     GIVSEKDLQAGLFESIGNDFI"
     gene            complement(1928..2899)
                     /locus_tag="PLX80_10940"
     CDS             complement(1928..2899)
                     /locus_tag="PLX80_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY21347.1"
                     /translation="MSSNNSYQKRILITGAAGFLGSHLSDRFIKEGYHVIGMDNLVTG
                     SKDNIAHLFPLKEFEFYFHDVTKFVHVPGKLDYILHFASPASPIDYLKIPIQTLNVGS
                     LGTHNLLGLAKEKKARFLIASTSEIYGDPMVHPQNEEYWGNVNPIGPRGVYDEAKRFM
                     EAITMAYHTYHGLETRIIRIFNTYGPRMRLDDGRALPAFFGQAIRGEDITVFGDGSQT
                     RSFCYVDDLVEGIYRLLLSEYANPVNIGNPDEITIKEFAEEIIKLTGTDQKIIYKTLP
                     TDDPKQRQPDITRAKELLGWEPKVPRAEGLKITYEYFKNVVNTSETV"
     gene            3020..3400
                     /gene="queF"
                     /locus_tag="PLX80_10945"
     CDS             3020..3400
                     /gene="queF"
                     /locus_tag="PLX80_10945"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HQY21348.1"
                     /translation="MKKINPIETDPNKVKLLETFDNSFPDRNYMIIHRVNEFTSVCPK
                     TGQPDFGVLTISFIAKKKCIELKSLKYYLQAFRNEGIFYENVTNRILDDLVNALKPKW
                     MEVKGEYSIRGGLNSTVIATYGKK"
     gene            complement(3419..4426)
                     /locus_tag="PLX80_10950"
     CDS             complement(3419..4426)
                     /locus_tag="PLX80_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY21349.1"
                     /translation="MKPENILEINDLKVKFPVTGGLLLRKIAEVKAVDGVSLELKRGE
                     TLGLVGESGCGKSTIGKAVINILRFSSPDVEITGEVWLKTEKGEVDLNKLSRNEMRQH
                     RGLIQMIFQDPYSSLNARMSVGQIIEEPLLYHTNMNKKEREERVAWLLEKVGLQAEQS
                     KRYPHEFSGGQRQRIGIARALSTNPKIIIADEPVSALDVSIQAQVINLLQQLQEEFDL
                     TILFIAHDLSVVEHISSRIAVMYLGNICELGGGYRVYHNPIHPYSRALLSAVPLPNPK
                     LKGKERVILKGDVPSPMNKPSGCGFRTRCPIAKPECADFIPILEEKEEQHYAACPYVS
                     A"
     gene            complement(4479..5549)
                     /locus_tag="PLX80_10955"
     CDS             complement(4479..5549)
                     /locus_tag="PLX80_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21350.1"
                     /translation="MEKKKLLEIKNLRTYFFVEDTEKHKALHEELTDPRLKELYASDK
                     YLKNKVPAKAIDDVSFNIYEGEVLGIVGESGSGKSVTAYSITRLIPDPPGKIVSGEVI
                     FKGKDLLKISFDEMKKYRGNEISMIFQEPMTSLNPVMKIGDQIIEAILNHEDISKDDA
                     VKKGIEMLTLVGIPSPEKRMKDYPHQFSGGMRQRVMIAMALSLHPSLLIADEPTTALD
                     VTIQAQILELMLRLKKEREDSAILLITHNLGVVAETCDRVIVMYGGKIQEISDVGPLF
                     ENPLHPYTMGLLSSLPDPDAPLEELKAIPGNIPHILELPKACKFNTRCTKAFDKCFEV
                     EPELIEFESNRFVRCHLYENEN"
     gene            complement(5674..6735)
                     /locus_tag="PLX80_10960"
     CDS             complement(5674..6735)
                     /locus_tag="PLX80_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY21351.1"
                     /translation="MSENKPEKNVSTSILQKRWKKFKTLKRGYYSFLLILILYILSFL
                     LPFFIGRDALIVSYNGELYFPIFKYHSAESMGQDLQGEANYRLLKKQYAQEDNGNWVL
                     LPLYPFGPNENLLDEMEGNPPTAPTSTHWCGTDDRGRDVFARLAHGFNIGLSFSIIVT
                     FFTYIVGIIIGSMLGFYGGKIDIFGLRLIEIWSTLPFLYVIIIISSIVQPNFILLIFI
                     LTLFGWISITYYMRGEFYREKARDYVSAAVSMGAKNNKIIFSHILPNSLTPVISYAPF
                     AIVANIFSLVSLDFLGFGLPPPTSSWGQLMQQGLSNIDNWWLIVTPLLAMFFTLLTIV
                     FIGEAIREAFDPKVYSRLR"
     gene            complement(6784..7815)
                     /locus_tag="PLX80_10965"
     CDS             complement(6784..7815)
                     /locus_tag="PLX80_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY21352.1"
                     /translation="MTSYFIRRFLLIIPTFFGITLITFLILQFVPGGPIEMEIMKMRM
                     GGSQGGSESGSSGNTTQNIPQSAIDELNKFYGFDKPITERYTIWVTNLLKFDLGKSYV
                     YAEPVTDVIFSRFPISIYFGLIGFILTYIICVPLGVYKAVKNGSTFDFVSSALVFIGY
                     AIPGFAFGALMLVLLGGGSFWDVFPLGGFRADDFESLSFMGKVKDQINHTVLPVISYL
                     IGSFATLTILTKNSVIENLSQDYIRTAFSKGLSEKRVIFGHALRNSLIPISTGLGHAI
                     SLVLAGSVLVERVFNINGIGLLSFDSIVQRDYPVVMGILVISTLLLLLGNILSDIIYA
                     IVDPRIRFK"
     gene            complement(7892..>8318)
                     /locus_tag="PLX80_10970"
     CDS             complement(7892..>8318)
                     /locus_tag="PLX80_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21353.1"
                     /translation="ADDPNSTNWPGYKSDRVDELAIQYNLEYDKSKRVKMVREIDAII
                     SAQQPYVYGWGADYTRLLWHNKFGYPEWYLSRFDEYYGPTDYGVFSMWWIDPKKNAAY
                     EEAVKDKSKKLPVEEVDSKFWIDIAERENTGEVIKLNDN"
BASE COUNT         2764 a         1472 c         1414 g         2668 t
ORIGIN      
        1 cgttacctaa aggaataata tgctggttat ttttttcatt attttctttc aaaaatcata
       61 tcttccttta aatatcataa tatcgaattt tttcatcata gtttctaaaa ttcaatttca
      121 aaataattat tatgattcta attaacagtt aaaacttaat ttttacaaat ggttttaatt
      181 ccgaaaaaaa ttaaacttta aatttgcaat gtgaatcaat aaattgctaa ctgaatttta
      241 aatttaaatt acaatttttt gaaaggtata attttagccg gaggcgccgg atcacgtttg
      301 tatcccgtct caaaagttta cagtaaacag cttacactta tctatgacaa accgcttatc
      361 tattatccgt tatcaattct catgttgtcg aatattaaag agatactgat catatcaaat
      421 gaagagacca ttccgctgta tcagaaatta tttgaagacg gtgcagacta tggtatcaaa
      481 atcgaatatg ctttacagac agctccaaat ggaatcgctg agtcatttat tatcggtgag
      541 aagtttatag gaaatgacgg tgcaactttg atacttggtg ataatatttt ctacggaaat
      601 cttgatttca tttacagagc aatgcagaaa aatgacggag ctacaatatt cggctataga
      661 gtgaatgatc cggaaagata cggtatagtt gagtttgaca ataccggtaa ggcaatttca
      721 atagaagaaa aacctgataa tccaaaatca aattttgcag ttccaggact ttatgtatat
      781 gataatgaag tagttgaaat ttccaaaaac attaagccgt cggccagagg cgagcttgaa
      841 ataacggatg tgaataatgt ttatttacag aaaggtaagc ttaatgtgga gaagatcggc
      901 aggggagttg cttggttaga tacgggaaca cctgaatcac ttttgcaggc atcgaatttt
      961 tttggagtaa tagaagacag acagggattg aaggttgcat gcattgaaga gattgcttac
     1021 cataaaggat ttataaataa ggatgaattt gaaagatcaa tttcgaaaat accgaattca
     1081 ctttataaga cttatctgga aaaagtcctc aaagaatatt aatttttaaa attgaatata
     1141 ataaagactg aagtagatgg tgtgctgata attgagccgg atttttttcc tgatgcaaga
     1201 ggattctttt ttgaatctta taacaaaaag aattataatg aagcgggaat aaatgctgaa
     1261 tttgttcagg ataatatttc taagtcagta aaaggaacga tccgcggact tcattatcag
     1321 gtgggagaat ttgctcaggg aaaactttgc caggtaatat ctggaagtgt gcttgacgta
     1381 gctgtcgata tcagaagcgg ttctccgact tatggtaaac atgtttcgat tgaactgaca
     1441 ggtaaaaatc ataaacagat atggataccg gcaggctttg cacatggctt ttcagttctt
     1501 tccgatgaag caattttttc ctataaatgt actgcacttt acagtaaaaa agatgagagg
     1561 tcgatcctgt ataatgaccc ggctttgaat attgactgga aattatcgga aggtattgtt
     1621 tcagagaaag atcttcaggc cggattattt gaaagtattg gaaatgattt tatttaactg
     1681 aaataagatc ttattatttc aatataaaaa aatccggctg taaattttat acagccggta
     1741 ttatttaatt tttgaagtta cgaaatttat ttttgaattc ttttgaaaga agttttacga
     1801 aacggggttt cgtctgaatt ttgcattccc aaactgtagt ttggaaacga gcatttttag
     1861 gtctgtattt aattaatgtt tcttgtgtaa ttaaagttaa tatttaaaat ttaatattaa
     1921 aatttattta aaccgtttca ctggtgttaa ctacattctt aaaatactca taagtaattt
     1981 tcagaccttc agctcttgga actttcggct cccatcctaa caattcttta gctcttgtaa
     2041 tgtcaggctg tctctgcttt ggatcgtcgg ttggtaaagt tttgtaaatt attttctgat
     2101 cagttccggt caatttaatt atttcttcag caaattcttt tattgtaatc tcatccggat
     2161 ttccgatatt cacaggatta gcatattcac ttaacagtaa tctgtaaatt ccttccacca
     2221 gatcatctac ataacagaat gatcttgtct gactgccgtc tccgaataca gtaatatctt
     2281 cacctcttat cgcctgtccg aagaaagccg gcaaagctct tccgtcatcg agtctcattc
     2341 tcggaccata cgtattaaaa attctgatga tccttgtttc aagtccgtga taagtatgat
     2401 aagccatggt gatagcttcc ataaatcttt ttgcttcatc atatactccg cggggaccga
     2461 ttggatttac gttaccccaa tattcttcat tctgcggatg caccatcgga tcgccgtata
     2521 tctctgaagt cgatgctatg agaaatcttg ctttcttttc ttttgcaaga ccaagtagat
     2581 tatgagtgcc gagagagcca acgtttaaag tctgtatagg aattttcaaa tagtctatag
     2641 gactggcagg agatgcaaaa tgtaatatgt agtcaagctt gccgggtaca tgtacaaact
     2701 ttgtaacgtc gtgaaaataa aattcaaatt ctttcagagg aaaaagatga gctatattat
     2761 cttttgaacc ggtaacaagg ttatccatgc caatgacgtg atatccctct tttataaatc
     2821 tatcgcttaa gtgtgaacct agaaatcccg cagcacctgt aataagaatt cttttttgat
     2881 atgagttatt tgatgacatt taaaatttta aatttttaat attgatttaa gagaaagcaa
     2941 tttacaaaat atgctgaaaa gttttcagat ataaataatt ctaactttat atttaattct
     3001 tcattcaata atttgcgtca tgaaaaaaat caatccaata gaaacagatc cgaacaaagt
     3061 gaaattactt gagacatttg ataattcatt ccctgacaga aattatatga taattcacag
     3121 ggtaaatgaa tttacatctg tctgtccaaa aaccggtcag cccgattttg gtgttctgac
     3181 aatttccttt attgcaaaaa aaaaatgcat tgagctgaag tcactgaaat attatttgca
     3241 ggcattcagg aatgaaggaa tattttatga gaatgttaca aacagaattc ttgatgatct
     3301 tgttaatgca ttgaaaccaa agtggatgga agtaaaaggg gagtacagta taagaggagg
     3361 actaaactct acggtaattg caacttacgg taaaaaataa ttccattttt aaaaaagatc
     3421 aggcagatac ataaggacat gctgcataat gttgttcttc tttttcctca agtatcggaa
     3481 taaaatcggc acattcaggt tttgcaattg gacatcgggt tcggaatccg cagccgcttg
     3541 gcttattcat tggtgaagga acgtcgcctt tgaggattac tctttcttta cctttcagtt
     3601 ttggatttgg caagggtact gctgacagca aagctcggct gtatggatga atcggattat
     3661 gataaactct gtatccgcct ccgagttcac aaatattgcc aagatacata actgcaattc
     3721 ttgagctgat atgctcgacg acagaaaggt catgagcgat aaataaaata gtgagatcaa
     3781 attcttcctg aagctgctgc agcagattaa ttacctgagc ctgaatagaa acgtcaagtg
     3841 cagataccgg ctcgtcagca attatgatct ttggatttgt tgacaatgct cttgcgattc
     3901 cgatcctttg tctctgacca cctgaaaatt catgcggata tctttttgac tgttcagcct
     3961 gcaggccgac tttttccagg agccaagcga ctctttcttc tctttctttt ttattcatgt
     4021 tagtatgata gagaagaggc tcttctataa tctgaccgac tgacatccta gcattaagag
     4081 aagaatatgg atcctgaaat atcatttgta tcaaacctct gtgttgtctc atttcatttc
     4141 ttgagagttt attcagatcg acttcgcctt tttcagtttt aagccagacc tctccggtga
     4201 tctccacatc gggagatgaa aatcttaaaa tatttataac ggctttacca attgtagatt
     4261 taccacatcc ggattcgcct acgagtccga gagtttcacc tctttttaat tcaaggctga
     4321 caccgtccac agcttttact tcagctattt ttcttaataa taaaccacct gtcactggga
     4381 attttacttt aagatcattt atttctaaaa tattttcagg tttcaattga ataattatct
     4441 gatttcaaaa tgatatatgg atatataaaa atattaaatc aattttcatt ttcatacaaa
     4501 tgacatctaa caaatctatt actttcgaac tcaatcagct cgggctcgac ctcaaaacat
     4561 ttatcaaaag ctttagtaca tcgggtatta aatttacagg cttttggtaa ttcgagaata
     4621 tgaggaatat ttcccggtat tgctttaagc tcttcaagcg gagcatccgg atcgggcaga
     4681 gatgacaata aacccatcgt ataaggatga agaggatttt caaaaagagg acccacatca
     4741 gatatttcct gaatttttcc gccatacatt acaattaccc tgtcgcaggt ttcagcgaca
     4801 acaccaaggt tatgtgtaat cagaagtatt gcagaatctt ctctttcctt tttaagtcta
     4861 agcatcagct caagtatctg agcctgaata gttacatcga gagcagtagt aggctcatca
     4921 gctataagta aagacggatg caaagataaa gccattgcga tcataactct ctgcctcata
     4981 cctccgctga actgatgagg ataatctttc attctttttt cgggagaggg aataccaacc
     5041 aatgtcagca tttcaattcc tttttttaca gcatcatctt ttgatatatc ttcatgattt
     5101 aaaatagctt caataatctg atcaccaatt ttcattacag gatttagaga agtcataggt
     5161 tcctgaaaaa tcatagagat ctcattgcct ctgtattttt tcatttcatc aaaggaaatc
     5221 ttcagaagat ctttcccttt aaaaatgact tcaccgctga caatcttacc cggtggatct
     5281 ggaataagtc tggtaataga atatgcagtt acagatttcc ctgaaccaga ttcgcctaca
     5341 atacctaaaa cttcaccttc ataaatatta aaactcacat catcaattgc tttagcaggt
     5401 actttatttt ttagatattt atctgatgca tataattctt ttaatcgggg atcagttaat
     5461 tcttcgtgaa gagctttgtg cttttctgta tcttcaacaa aaaaataagt acggagattt
     5521 tttatttcaa gaagcttttt cttttccatt taaagaatta ataaaataat aaacattttg
     5581 ttcaattata aatttacaca aaatttttca acacaataaa ctttagcagc agtccggtat
     5641 agtagacttt acaattaaaa tactctaaca attttatctg agtcttgagt atacttttgg
     5701 atcaaaagct tctctgatcg cttcaccgat aaatacaata gtcagcaaag taaaaaacat
     5761 tgctaacaaa ggagttacga tcagccacca gttgtcaata ttacttaatc cctgctgcat
     5821 gagctgaccc cagcttgatg tcggaggcgg taacccgaat ccgagaaaat caagagatac
     5881 gagtgaaaaa atatttgcaa ctatagcaaa gggagcgtaa gaaattacag gtgtaagaga
     5941 atttggtaaa atatggctga aaattatttt attgttttta gctcccatag aaacagcagc
     6001 agatacgtaa tctctggctt tctcacggta aaattctcca cgcatataat aagttatact
     6061 tatccatcca aaaagtgtaa gaataaagat cagtaaaatg aaattcggct gtacgataga
     6121 actgataata atgatgacat acaagaaggg gagtgtgctc cagatctcaa taagtctgag
     6181 accaaaaata tcaattttcc ctccatagaa tccgagcatg gatccgataa tgataccaac
     6241 tatgtaagta aaaaaagtaa ctataattga aaatgaaagt ccgatattaa atccatgtgc
     6301 aagccttgca aaaacatctc tgcctctgtc atcggttccg caccaatgtg tggaagtcgg
     6361 cgctgtaggt ggatttcctt ccatttcatc taaaagattt tcattaggtc caaatggata
     6421 caaaggcagc agtacccagt taccattatc ttcctgtgcg tactgtttct ttaaaagtct
     6481 gtaatttgct tcgccctgaa gatcctgccc catagattca gcggaatggt acttgaatat
     6541 tggaaaatat aattcaccat tataacttac aatcagggca tctcttccaa taaagaacgg
     6601 aagtaaaaaa gagagaatat ataaaattaa gataagtaaa aaagaataat aacctctttt
     6661 cagtgtctta aattttttcc atcttttctg aaggattgat gttgaaacgt ttttctcagg
     6721 tttattttct gacataaaaa atttaatgtt acttgatttc aaaaacggat tgcgtaaacc
     6781 ggactattta aatcgaatcc ttggatctac tattgcataa ataatatcag ataaaatatt
     6841 acctaataaa agtaaaagtg tagatattac aagaattccc attacaacag gataatctct
     6901 ctgaacaatg gaatcaaagc tcagaagacc aattccattg atattaaaaa ctctttcaac
     6961 aagtacacta cctgctaaga ccagtgaaat agcatgtcca agtccggttg agatcgggat
     7021 aagtgaattc ctgagtgcat gtccgaaaat aactcttttt tcagaaagcc cttttgaaaa
     7081 agcagttcgg atataatcct gactcagatt ttctattacg gaattttttg ttaaaatagt
     7141 aagagttgca aagcttccaa ttaaataact aattacgggt aagacagtat ggttgatctg
     7201 atccttaact tttcccataa aagaaagaga ttcaaaatca tcagctctga atccaccaag
     7261 cggaaataca tcccaaaaac ttcctccgcc gagcaataca agcatcaaag caccaaacgc
     7321 aaacccgggt atagcatagc caataaaaac aagggcagat gaaacaaaat caaatgtgga
     7381 tccattttta acagctttat aaactcctaa aggaacacag ataatataag tgagaataaa
     7441 tccaatgaga ccaaaataaa tcgatatagg aaatctcgag aaaattacat ctgtaaccgg
     7501 ctctgcataa acataagatt ttccaagatc aaatttcaaa agatttgtaa cccagatagt
     7561 atatctttca gtaataggtt tatcaaaccc ataaaattta ttcagttcat caatagctga
     7621 ctggggaata ttttgagttg tattacctga gcttccggat tctgatccac cctgagatcc
     7681 gcccatccgc atcttcataa tttccatttc gatgggacct ccaggaacaa attgcaggat
     7741 cagaaaagtg atcagagtga taccaaaaaa agtcgggatt attagcagaa atcttcttat
     7801 aaaataagaa gtcataagat taaattttta tttcattaat aaaaaaaata taaaaaaaaa
     7861 caggcagtat aaaaaaactg cctgcatata attaattatc gttaagttta attacttctc
     7921 cggtgttttc tctttcagca atatcaatcc agaatttact gtccacttct tctacaggta
     7981 atttcttgga cttatcttta actgcttctt cataagcagc attttttttc ggatctatcc
     8041 accacatgga aaatactccg tagtctgtcg gaccataata ttcatcaaat ctggacaaat
     8101 accattccgg ataaccgaat ttattatgcc acaataatct tgtataatca gctccccatc
     8161 catatacata tggctgctgt gcagaaataa tagcatcaat ttctctgacc atctttactc
     8221 tttttgattt atcatattcg agattatatt gaatggcaag ttcatcaaca cgatcgctct
     8281 tgtatccggg ccagttagta ctgttagggt catcagca
//
LOCUS       DAORVQ010000147         8237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4964_length_8237_cov_4.849181, whole genome shotgun sequence.
ACCESSION   DAORVQ010000147 DAORVQ010000000
VERSION     DAORVQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4964_length_8237_cov_4.849181"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..700
                     /locus_tag="PLX80_10975"
     CDS             77..700
                     /locus_tag="PLX80_10975"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQY21354.1"
                     /translation="MFTGIVQNIGEIILETPVGDSIIFEISVPESDFTDGLKSGDSIA
                     VNGVCLTATNIEKNKMKFTAVKETLTKTNLGELTKGSFVNLEKAMSIDSKLDGHLVQG
                     HVDTTGKVVGIIENIDSHEFLIEFDSKFRDYIIKIGSVCINGVSLTIAEIVKENENEN
                     KIIIKIAIIPFTNNNTTFQYLRINNSVNIEFDMMGKYIKRIFENSIK"
     gene            704..985
                     /locus_tag="PLX80_10980"
     CDS             704..985
                     /locus_tag="PLX80_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21355.1"
                     /translation="MAFESDKTMFEIYREKEFNRKFKVVYYTELTEHNKEFEINSALN
                     GETLFDGFLIDVKKEEGKTAVSEIINEMNKNDSALNSDEIISRLKEYLA"
     gene            complement(977..2017)
                     /locus_tag="PLX80_10985"
     CDS             complement(977..2017)
                     /locus_tag="PLX80_10985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY21356.1"
                     /translation="MSEINMQVKKILIIQTAFLGDVILTIPLIQEIKVKYPDSIIDFL
                     CIPSTSDLLKNNPDIRELIPYDKRNTGFRGLVEIIKKIRGESYDYLISPHRSFRSAMI
                     SHFSNSGMTISFDKSTFSFLYDEKIKYETGIHEIQRNLKLLSPLGIKVDNIVRPELFI
                     NIQEKRKIDSIFYEYRIRENEKILTIAPGTIWFTKRFPEEKFIKLCNLLTGINSRIFI
                     IGSKNDKTISDFIVNNSKFRNFINLAGTLSVMESAELIRRSELLVSNDSAPMHLANSV
                     ETDVCAIFGATVPEFGFFPYGKRDKIFQINDLKCRPCSIHGGSKCPIKTFNCMYNIDE
                     NEIAEFLIERLS"
     gene            complement(2004..2795)
                     /locus_tag="PLX80_10990"
     CDS             complement(2004..2795)
                     /locus_tag="PLX80_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY21357.1"
                     /translation="MFRAVFFYLIPIRKKVAYNNLDLCFPNKDHSFKKLIVRENYINL
                     GIDLLEFFYFPDLTIEKINKTTEFHDMNVIEDAGKAGNGTLFLSGHYSNWEFIAFSFS
                     KLFKYSLKIIAKPQGSRSLNKKINSYRTLGGNEIIQTGISLRAIFKSLDQNDFICFLI
                     DQSAHPDYSVYVNFLGQKTASFSGPAKIALKKRCELILGYGIRDKNYKYDVYFSKIDY
                     SDIVDYNEENIIVLTQRIQTEFEKIVLADPGQWLWLHKRFKHVRN"
     gene            complement(2889..4409)
                     /gene="miaB"
                     /locus_tag="PLX80_10995"
     CDS             complement(2889..4409)
                     /gene="miaB"
                     /locus_tag="PLX80_10995"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQY21358.1"
                     /translation="MYRSGIFSIIQRPENIRIQAINISMEITNLILNNVPSPEIKNNF
                     NKLSNDKKVYIETYGCQMNFSDTEIVLSVLSNKGYNETSDINESDVILLNTCSIRENA
                     ENRIYSRLGDLIKYKKKNPELVIGILGCMAERLRDDLLKKKKIVDIIVGPDEYRKVPE
                     LIENSIEKNEKGIAVKLSRTETYDDITPLRREGITAWLSIMRGCDKFCSFCIVPFTRG
                     RERSRNLNNIVDETKRLYNEGVKDIWLLGQNVNSYKHEDKDFADLLRSVAVAVPEMRV
                     RYTTSHPYDLSDKLLDTMAEFDNICKYIHLPIQSGSDRVLKNMNRLYSVKEYMNVMLK
                     AREMMPGIGLSTDIISCFPGETEDEHQMTMDVIRQIEYDSAFTFVYSPRENTKAYELK
                     DKLDEETKKKRLDEIITLQREISYKINSSLKGKIMEVLVESLSKKSNEEYMGRTDCNK
                     SVIIPRNYNAVNDSGTLINDGAINSGDLINVEITGANSATLFGNPVYTLKNSNSKN"
     gene            complement(4346..4816)
                     /locus_tag="PLX80_11000"
     CDS             complement(4346..4816)
                     /locus_tag="PLX80_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21359.1"
                     /translation="MKKYKYIYQSLLLLISLTVFQSCSDNSVQNVEEGELIYSHDGII
                     EEVGGDCSAVQVRTRSFGEFDLSDFSKIRFELNGSSDADLSSLSLFYYENDEIIYLVN
                     LTNRDEINSTRNIEINSPRSSREIFSRVTLKSSVCTGQVFFLSFRDLKIFAYRQ"
     gene            complement(4803..6719)
                     /gene="dxs"
                     /locus_tag="PLX80_11005"
     CDS             complement(4803..6719)
                     /gene="dxs"
                     /locus_tag="PLX80_11005"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQY21360.1"
                     /translation="MSEYNINNTKFLKDIDYPKDLKKLNLMNLRELADEVREYLIDTI
                     SRIGGHLGASLGVCELTLALHYVFNTPDDKIIWDVGHQAYIHKILTGRKEQLKTIRQK
                     NGISGFLKRSESEYDVFGAGHATTSISAALGIATARDFSNKNFKVISVIGDGSMTGGM
                     AYEAMNNIGLLKKDIIVVLNDNKMSIAPNVWSLQNYFNDLMTNDTYNKFRSKVWELTE
                     VFDKKNSDRLRKVGSKIEGSIKSVVTPGMLFEALGFRYFGPVNGHNVVNLVKMLEELK
                     RMSGPILLHIISEKGKGPSYASDHPQKIHALNPFDKSTGIEHKKSTVSTYTTEFGTAL
                     VELAKKDKKIIAVNAAMPDGTGLNIMQKEIPDRYFDVGIAEQHAVTFCAGLATEGYTP
                     VAAIYSTFLQRAFDQIIHDVATQKLPVIFVLDRGGLVGADGPTHHGVFDLTYLRLIPG
                     MVLMSPKDENELRNMLFTATKYKRGPVAIRYPRGNALGVKKGEFELMEIGKGEIVKKG
                     KDVAILAVGNMVENSLNSEKYLEEYGINAEIINARFIKPLDRELMYDVFKRFDKIITI
                     EDNTIVGGFGSAVAEFAAENNLKSDILIHGIPDRFIDHAKPEELHEDLRIDAKGIAEV
                     VKEFLSKEKIHEKV"
     gene            complement(6749..6958)
                     /gene="xseB"
                     /locus_tag="PLX80_11010"
     CDS             complement(6749..6958)
                     /gene="xseB"
                     /locus_tag="PLX80_11010"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQY21361.1"
                     /translation="MKKKIEDTFGNNFKKLEEILVNLESGIEDHSLEEIIKNYQDGIN
                     LINICRKKLDEAELKIEKISTEQDN"
     gene            complement(6963..8219)
                     /gene="xseA"
                     /locus_tag="PLX80_11015"
     CDS             complement(6963..8219)
                     /gene="xseA"
                     /locus_tag="PLX80_11015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQY21362.1"
                     /translation="MNSPLTVSELSRDLKNTIERNFDYVFVLGEISNFKRHFSGHIYF
                     LLKDSKAQINAVIWKARADLLNIFPADGMKVLIKGRITLYETRGSYQIDVFNIQPAGE
                     GELQIAFENLKRKLEFEGLFDSKYKKSLPEFPERVAVITSETGAAIQDFLKVTERRYP
                     VVTIYLINAGMQGSGSANSVCKAIAVANKIKNKPEIIVITRGGGSIEDLWTFNEEKVA
                     RAVFASEIPVVSAIGHEVDFTICDFTADVRASTPSAAAELIFPVLLNIQLSLNDIENN
                     FKRKVLTEIKDLKSSLNKIENNYYFKKPADMINEYKMNLDNIEQKLADNAKKKLNEIK
                     IRLERISKELIFIPGRSILNLKNLLNSNEKLLNSLNPENVLKRGFAIIKMGDKIISYK
                     KQLNVNDNIQINFNDGEADAVIDKLK"
BASE COUNT         2700 a         1418 c         1119 g         3000 t
ORIGIN      
        1 cattaaacaa aacagtagat cttaaaacag aatttacaaa aagaattgga aaagatattt
       61 taattaaata caaaaattgt ttacaggtat agttcaaaat attggcgaaa ttattttaga
      121 aactccggtt ggtgacagca taatatttga gatttctgtt cctgaatctg attttacaga
      181 tggattaaaa tcgggtgaca gtatagcagt aaacggtgta tgtctaactg ctacaaacat
      241 tgaaaagaat aaaatgaagt ttactgcagt aaaagaaact ctgacaaaga ctaaccttgg
      301 agaacttaca aaaggaagtt ttgtaaactt agaaaaagca atgtctatag attcaaaact
      361 tgacggacat cttgtacagg gacatgtaga taccacagga aaagttgttg gaataattga
      421 gaatattgat tcgcatgaat tcctgataga atttgattct aaattcagag attatattat
      481 taaaatcgga tctgtttgta taaatggtgt tagtcttacg atagctgaga tagttaaaga
      541 aaatgaaaat gaaaataaaa taataattaa aattgcaata atcccattta caaataacaa
      601 tactacattt caatatttaa gaattaataa ttcagtcaat attgaatttg atatgatggg
      661 gaaatacata aaaagaattt ttgaaaacag cataaaataa aaaatggctt ttgaatctga
      721 caaaacaatg ttcgaaatat acagagaaaa agaatttaac agaaaattca aagtagtata
      781 ttatacagaa ctaactgaac acaacaagga atttgagatc aacagtgctt tgaatggaga
      841 aacacttttt gacggatttt taattgatgt aaaaaaagaa gaagggaaaa cggcagtttc
      901 tgagattatt aatgaaatga ataaaaatga ttcagcatta aattcagatg aaataataag
      961 caggttaaaa gaatatttag cttaatcttt caatcaaaaa ttctgcaatt tcattttcat
     1021 ctatattata catacaatta aaagttttga tcggacattt gctgcctccg tgaatactgc
     1081 agggtctgca tttaagatca ttaatctgaa atattttatc tctttttcca taggggaaaa
     1141 aaccaaactc aggaactgtt gctccgaata ttgcacaaac atctgtttcc actgaatttg
     1201 cgagatgcat tggcgcagaa tcgttcgaaa ccaataattc tgatcttctg ataagttcag
     1261 cagattccat taccgataaa gtacctgcaa gatttataaa atttctgaat ttagaattgt
     1321 ttacaataaa atcggaaatc gttttatcat ttttacttcc tattataaat attctggagt
     1381 ttattcctgt taacagatta caaagtttta taaatttttc ttcagggaat ctctttgtga
     1441 accaaatagt acccggagca atagtaagta tcttttcatt ttctcttatc ctgtattcat
     1501 aaaaaatact gtcaattttt cttttctcct gaatattaat aaacaattct ggtctgacaa
     1561 tattgtcaac tttaattcct aatggtgaca gcagttttaa atttctctga atctcatgaa
     1621 tacctgtttc atattttatt ttttcatcat ataaaaaact aaatgttgat ttatcaaaag
     1681 agattgtcat gcctgaattt gaaaaatgac ttatcatagc tgatctgaat gatctgtgag
     1741 gtgagattaa ataatcatat gattcccctc ttatcttttt tatgatctca acaagtcctc
     1801 tgaaaccggt atttcttttg tcataaggta taagctctct gatatccgga ttatttttca
     1861 aaagatctga agtcgatgga atacataaaa aatcaataat ggaatcagga tactttactt
     1921 taatttcctg aatcaatgga atagtaagaa tcacatctcc caaaaaagca gtttgaataa
     1981 ttaatatttt tttaacctgc atgttaattt ctgacatgtt taaatctttt atgaagccat
     2041 agccattgcc ccggatctgc caaaactatt ttttcaaatt ctgtttgtat cctttgagta
     2101 agaactatta tattttcttc attataatct acaatatcgc tgtaatcaat tttagaaaaa
     2161 taaacatcat atttataatt tttatctctg atgccatatc ctaatatcag ttcacatctt
     2221 tttttaagtg caatttttgc gggtccggaa aagctagctg ttttctgacc taaaaaattc
     2281 acataaactg aatagtccgg atgagctgat tgatcgatga ggaagcatat aaaatcattt
     2341 tgatcgagac ttttgaaaat tgctcttaat gaaataccag tctgaataat ttcattgccg
     2401 ccaagtgtcc ggtaagaatt aattttttta tttaatgatc tgctgccttg tggtttggca
     2461 attattttca aactatattt aaataatttt gaaaatgaaa acgcaataaa ctcccagtta
     2521 gaataatgac cacttaaaaa aagtgttccg tttcctgcct ttcctgcatc ttcaataaca
     2581 ttcatatcat gaaattcagt agtcttatta attttttcta ttgtaagatc aggaaaataa
     2641 aaaaactcaa gcagatctat tccaagatta atataatttt ccctgacaat taatttctta
     2701 aaggaatggt ctttattagg gaaacacaga tcaagattat tataagctac cttttttctg
     2761 ataggaatca gataaaaaaa aacagcccta aatattttcc tgagcgttga atgaatttta
     2821 aaggaaagag tcttatcaga aaacctgcag tattaaaaaa aaatattgaa attaaattca
     2881 ccaatacttt aatttttcga gttagaattc tttaaggtat aaacaggatt tccaaataaa
     2941 gtagcagagt tagcacctgt aatttcgaca ttaataagat cacctgaatt aattgcaccg
     3001 tcatttatta aagtgccgga atcgttaacg gcattataat ttcttggaat aataaccgac
     3061 ttattacaat cagttcttcc catatattct tcatttgatt ttttactaag tgattctacc
     3121 agtacttcca ttattttacc ttttaaagaa gaattaattt tatatgagat ctcacgctgt
     3181 aatgtaatta tttcatccaa tcttttcttc ttagtttctt catctaattt atcttttagc
     3241 tcatatgcct tagtattttc tcttggtgaa tagacaaaag taaaagcact gtcatattca
     3301 atttgcctga tcacatccat tgtcatttga tgttcatctt cagtctcgcc cggaaaacat
     3361 gaaattatat ccgtagaaag tccaataccc ggcatcatct ctctcgcctt aagcataaca
     3421 ttcatatatt ctttcacaga atataatctg ttcatatttt ttaaaactct gtccgaacct
     3481 gattgaatcg gaagatgaat gtatttacaa atgttatcaa actctgccat tgtatctaaa
     3541 agtttgtcag acagatcata tggatgagaa gtcgtatacc tgactctcat ttcaggaact
     3601 gcaacagcta ctgatcttaa tagatcagca aaatctttat cttcatgttt gtaagaattt
     3661 acattctgtc caagcagcca aatgtctttg acaccttcat tatatagtct tttggtttca
     3721 tcgactatat tattcaaatt tctgctacgc tctctgcctc ttgtaaaagg tactatgcag
     3781 aatgaacaaa acttatcaca ccccctcatg attgacagcc aggctgttat accttcacgt
     3841 ctcaaaggag taatgtcatc ataagtttca gttcttgata attttactgc aatccctttt
     3901 tcatttttct caattgagtt ttcaattaat tcaggcactt ttctgtattc atcgggacct
     3961 acaattatat ctacaatttt ctttttttta agaaggtcat ctcttaatct ttctgccata
     4021 cagccaagaa ttccaatcac cagttcagga tttttctttt tatattttat aagatctcca
     4081 agtctgctgt atatcctgtt ctctgcattt tctctgatac tacatgtatt taagaggatt
     4141 acatcggact cattaatatc agaagtttca ttatatcctt tattacttaa tacactgagc
     4201 acaatttccg tatctgaaaa attcatctga catccgtaag tctctatata gacttttttg
     4261 tcattagata atttgttgaa attgttttta atttcgggag atggtacgtt atttaaaatt
     4321 agatttgtaa tttccattga tatgtttatt gcctgtatgc gaatattttc aggtctctga
     4381 atgatagaaa aaatacctga cctgtacata ctgacgattt taaagtaact cttgaaaaaa
     4441 tttctctgct ggatcgaggt gagtttattt caatatttct tgtagaattt atttcatctc
     4501 tgtttgttaa attaacgaga tatataattt catcattctc ataataaaac aaagatagac
     4561 ttgaaagatc agcatctgaa gaaccattta attcaaacct tatcttggaa aaatctgaaa
     4621 gatcaaattc accaaagctt cttgtcctga cctgcactgc agagcaatct ccaccaactt
     4681 cttcaataat accgtcatga ctatatatta attctccttc ctctacattc tgaacagaat
     4741 tatcactgca actctgaaag actgtcagac tgatcagaag caaaagagat tgataaatat
     4801 atttatactt tttcatgaat tttttctttt gataaaaatt cttttactac ttcagcaatt
     4861 ccttttgcat caattctcag atcttcatgc aactcttcag gttttgcatg atcaataaat
     4921 ctgtcaggta ttccgtgaat aagaatatca ctttttagat tattttcggc ggcaaactca
     4981 gctacagcac tgccaaatcc tcctactata gtattgtctt caatagtaat tattttgtca
     5041 aatcttttaa atacatcata cattaattct ctgtccaaag gcttaatgaa tctggcgtta
     5101 attatttctg cgttaatacc atattcttca agatattttt ctgaattaag agaattttca
     5161 accatatttc cgactgctaa aatagcaaca tcctttcctt tcttaactat ttcacctttt
     5221 ccaatttcca ttaattcgaa ctcacctttt ttcacaccca atgcatttcc cctcggatac
     5281 cttatagcta cgggacctct tttatatttt gtagcagtaa ataacatatt tcttaattca
     5341 ttctcatctt taggtgacat taataccatt ccgggaataa gtcttaaata tgtcagatca
     5401 aaaactccat gatgagtcgg tccgtcagct cctactagtc cacctctgtc aaggacaaaa
     5461 atgaccggta gtttttgagt agctacatca tgtatgatct gatcaaatgc tctttgtaaa
     5521 aaagtagagt aaattgctgc aacaggtgta taaccttcgg ttgcaagtcc tgcacaaaat
     5581 gtaacagcat gctgctcggc aatacctaca tcaaaatatc tatcaggaat ttctttctgc
     5641 ataatgttta atccggttcc gtccggcata gcagcattta ctgcaataat ttttttatct
     5701 ttcttagcca gttcgacaag tgcagttccg aattctgtag tatatgtaga aacagtagat
     5761 tttttatgct caatgcctgt agatttgtca aatggattta aagcatgtat cttctgcgga
     5821 tggtcagatg cataagatgg tcctttacct ttttctgaaa taatatgaag aagtattggt
     5881 ccggacattc tttttaactc ttcaagcatc ttaacaagat taacaacatt atgaccatta
     5941 actggaccaa aatatctgaa accaagagct tcaaaaagca ttccaggagt tactacactt
     6001 ttaatacttc cttctatttt tgatccaact tttcttaatc tgtcactatt ctttttatca
     6061 aatacttcag ttaattccca tactttgctt ctgaatttat tataagtatc atttgtcatc
     6121 agatcattaa aataattctg aagtgaccag acattcggag ctatcgacat tttattgtca
     6181 tttagaacta caataatatc ctttttcaaa agtccgatat tattcattgc ttcgtaagcc
     6241 attcctcctg tcatagatcc gtcacctatt acagatataa ctttgaaatt tttgtttgaa
     6301 aaatctctgg cagttgctat tccaagagct gcagaaatag aagtggttgc atgaccggct
     6361 ccgaacacat catattcact ttcacttctt ttaagaaaac cacttatacc gttcttctgt
     6421 cttatagttt ttaactgttc ttttcttccg gttaatattt tatgaatgta agcctgatga
     6481 ccaacatccc aaatgatctt atcgtcggga gtattaaata cataatgtag agccagagta
     6541 agttcacata cacctaatga tgctccgaga tgtccgccaa ttctggaaat tgtatcgatc
     6601 aaatattccc gaacttcatc tgcaagttct ctaagattca tcagatttaa tttctttaga
     6661 tctttaggat aatcaatatc ctttagaaat tttgtattgt taatattata ttcactcatt
     6721 gaaaaaattt gttataaata tacgattttt aattatcttg ttcagtgcta attttttcaa
     6781 tttttaattc agcttcatca agtttcttac ggcaaatatt aataagattt ataccatctt
     6841 gataattttt aattatttct tcaagtgaat gatcttcaat tccagattca agattaacaa
     6901 gtatctcctc taatttttta aaattgtttc caaatgtatc ttctattttc tttttcataa
     6961 atttatttta gtttatcaat tacggcatca gcttcaccgt cattaaaatt tatctgtata
     7021 ttatcattta cattaagttg ttttttatat gaaattattt tgtctcccat tttaataatc
     7081 gcaaatcccc ttttcaatac attttcagga ttaagtgaat ttagtaattt ttcatttgag
     7141 tttaataaat tttttaggtt gagaatactt ctacccggaa taaatatcag ttctttacta
     7201 attctttcta atcgaatttt aatttcattt aatttctttt ttgcattatc agctaatttc
     7261 tgttcaatgt tgtccaaatt cattttatat tcattgatca tatcagccgg ctttttgaaa
     7321 taataattat tttcaatttt atttaaactg gattttaaat cttttatttc agttaagact
     7381 ttccgtttaa aattattttc aatatcattt aagctaagtt gtatatttaa gagtaccgga
     7441 aaaatcagtt cggcagcagc ggaaggagtc gatgctctaa catcagcagt gaaatcacag
     7501 atggtaaaat ctacttcatg accaatagca gatacaacag gtatttctga agcaaataca
     7561 gctcgtgcaa ccttttcttc gttaaatgtc cacaagtctt caatagaacc tccacctctg
     7621 gtaataacaa tgatctcagg tttatttttg attttgttag cgacagcaat tgctttacag
     7681 acactgtttg ctgagcctga tccctgcata cctgcattaa ttaaataaat ggtcacaacc
     7741 ggatatcgtc tttcagttac ctttaaaaaa tcctgaattg ctgcaccagt ctccgaagtt
     7801 attacagcta ctctttcagg aaattcagga agagattttt tatattttga atcaaacaaa
     7861 ccttcaaact ctaattttct ttttagattt tcaaaagcaa tctgtagctc tccttcccct
     7921 gcaggttgaa tattgaatac atcaatctga tagctgcctc ttgtttcata taaagtgatt
     7981 ctccctttta ttagtacttt catcccgtct gctggaaaga tgtttaaaag atcagctctt
     8041 gctttccata taacagcatt aatctgtgct ttactatctt ttagtaaaaa atatatatgc
     8101 cccgaaaaat gtcttttgaa atttgaaatt tcacccaata caaaaacata atcaaaattt
     8161 ctttcaatag tattttttaa gtctctggat aattctgata ctgttaaagg tgaattcaat
     8221 tttaagttta tttaatt
//
LOCUS       DAORVQ010000148         8218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4980_length_8218_cov_5.779738, whole genome shotgun sequence.
ACCESSION   DAORVQ010000148 DAORVQ010000000
VERSION     DAORVQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4980_length_8218_cov_5.779738"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLX80_11020"
     CDS             complement(<1..428)
                     /locus_tag="PLX80_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21363.1"
                     /translation="MKQLICIILLTIISCNNSPQKESSSDNTISDTSKSLPNTKINRY
                     SDICWTGTIDEKIPVFIRYQLDSNLIIGEITYLNSKDKLPIPLLGLIEEDKSYMLMEF
                     EKSGNISGIIRGYHTGEVFYGNWFSPEKRIEFVLNLVKKDT"
     gene            complement(602..1306)
                     /locus_tag="PLX80_11025"
     CDS             complement(602..1306)
                     /locus_tag="PLX80_11025"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HQY21364.1"
                     /translation="MIFLLSIAGGSVDAIIILSFNVLTAAQTGNTILLAVALAQGNFT
                     AGYFSAVSIAAYITGALLGESIIVSRKKSSSRLGPVGWTLFTELILLIILILFWLFFG
                     PVQTTIHTTILISLTACAMGIQSAAVLMLHSGPATTYVTGTITSFSTKLMWWLYHFGT
                     PVPRVINNSDSQDSGFWSGRNPWIFGISWIIYLCGAIGGAVVYLWVGLAAFVIPLICI
                     AVIIIIAAVSEREMGI"
     gene            complement(1490..2041)
                     /locus_tag="PLX80_11030"
     CDS             complement(1490..2041)
                     /locus_tag="PLX80_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HQY21365.1"
                     /translation="MDKATQTMIENLHKNSGKTLEQWIEIVKKKDFAKHGEIMKYLKE
                     EHGLTHGFANLVALKSRGTDAGSAENESDLIEKQYKGKEHFKPIYDKLISEIMKFGKD
                     IEIAPKNANVSLRRKKQFALLEPATKTRFEIGINIKGQESKGKLTAEKPGSMCTHKIK
                     IADIKEIDKEVIDWIRTAYENAG"
     gene            complement(2114..2518)
                     /locus_tag="PLX80_11035"
     CDS             complement(2114..2518)
                     /locus_tag="PLX80_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HQY21366.1"
                     /translation="MKQIFINLPVADVERSMNFYTQLGFTINPEFTFEDQKCMVWSDH
                     IYVMLMTQKMFLHYNRKKIPDTKDYMSASYTLPVESLDKVNEIMESGIKAGGTESIPV
                     IDEGFMQVRTIEDYDGHTWGIIFLDMEKFRNR"
     gene            2915..5053
                     /locus_tag="PLX80_11040"
     CDS             2915..5053
                     /locus_tag="PLX80_11040"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21367.1"
                     /translation="MKILLSLLTSLMLMIFFNQNTFSQQTDNWQWLNPKPQGNTIYAL
                     DFVNNSTGYAGGDFGTIMKTTDNGDTWTKLNTGFSDRLLSLDFIDDNTGFAGGDDQLL
                     KKTTDGGQTWTDVSLPSAPDFYIFDIDFVNSQTGYVLGFFVMESLIWKTTDGGLSWTT
                     QTTDGANYLNNLYFLDENNGFASGGTLGGQIIKTTNGGLNWDYNYQDNYAKHSMVFLN
                     SLTGYSGSDQGRIYKTIDGGNTWNFVLSDGGIDIMSMNFTDANTGFGFGTGSVYVKTT
                     DGGDNWYQSNQIGSSMNSQYFDASITPNGSIHAAGTFGAMVRSTNSGADFTYKVSVTD
                     GYVSDIEFININTGYAVTGYNHGDILKTTDAGASWVSQITTYTTPIYGISFTSNETGY
                     LAGSISLYKTTNGGTNWNTVYNSTTNEIFTDIVFTNANTGYAVGSYGRQMKTTNAGLN
                     WTASTISSSGSILSSLCFVNETTGYAVGDNNAAVRTTNSGLNWNSMSVASPFINLTDV
                     FFTDVNNGYISCTTGIYRTTNGGDSWFALSTPAGGYAKVQFRGNFGYSITGNGKIIKT
                     IDGGVNWIVQPTITTNGLSALYFNSDNFIYAGGLLGTMLKTIPTELIITHAGNINTEI
                     PGNFLLSQNYPNPFNPSTKITFEIPNSGLVTLKVFDITGKLVDVLLNGNKNSGKYTVD
                     FNGSSLASGVYFYSLTSNDFTDTKKMILMK"
     gene            complement(5298..5777)
                     /locus_tag="PLX80_11045"
     CDS             complement(5298..5777)
                     /locus_tag="PLX80_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21368.1"
                     /translation="MQINKVKLRQSEISDLEFFFQFQLDEEANYLAAFTSKDPADKAA
                     YFEKYTKHFNDPAINNMTILFGETIAGSIAKFVMEGDAEITYWIDKSFWGKGIATTAL
                     KIFLTLENTRPIFARVAFDNFGSQKVLEKCGFVKIGTDKGFANARQEETEEFIYRLT"
     gene            complement(5914..7116)
                     /locus_tag="PLX80_11050"
     CDS             complement(5914..7116)
                     /locus_tag="PLX80_11050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY21369.1"
                     /translation="MNLTSGYPYWLINSGLPNKFEKLYQDVKTDVVILGGGISGALTS
                     YYLINSGIDCILVDARTIGLGSTCASTSLLQYELDTPLCKLSKQIGFKKAARSYKLCS
                     ESIDTLNDISNKLKFKEFEKNNSLFYAAYKKHDELIHEEYLIRKKCGFQVEHLIENDI
                     EKGYGFSASSAILSEQGGTTDAYLFTHALLKESEKKGLRIYDRTNITEIEYKKRSVKL
                     RTENNCNITAKKIVNATGFEITEFINKKIVKLISTYALISENIKTPSPVWNDKILIWN
                     TDDPYLYLRLTKDNRVIIGGRDEDFSNAVKRDKLIKKKSTQLVKDFEKLFPEIELLPE
                     FRWTGTFGTTKDSLPYIGTYEKTPHTYYALGFGGNGITFSVIAAEIICDLIKSKRNAN
                     AELFSFQR"
     gene            complement(7470..7817)
                     /locus_tag="PLX80_11055"
     CDS             complement(7470..7817)
                     /locus_tag="PLX80_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HQY21370.1"
                     /translation="MEKHRIYKMSLASVYPHYITKAEKKGRTKEEVDTVTCWLTGYDK
                     KELQNHLDNRTDFETFFAQAKLNKNASKITGVICGYRVEEIEDKLMQKVRYLDKLVDE
                     LAKGKAMEKILRK"
     gene            complement(7964..>8218)
                     /locus_tag="PLX80_11060"
     CDS             complement(7964..>8218)
                     /locus_tag="PLX80_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQY21371.1"
                     /translation="QVHRAILEEDGQLSVFFFEDEEVKYGLPIIPEYIEHPLNSIPVE
                     GYYSCIDCSNTKVLQTGSLPVCNICSECKWVKAIKDKRIT"
BASE COUNT         2565 a         1440 c         1359 g         2854 t
ORIGIN      
        1 gtgtcttttt ttacaagatt aagcacaaac tctattcttt tctcaggtga aaaccagttg
       61 ccatagaata cctcgcctgt atgatatccc cttataattc ctgaaatatt tcctgatttt
      121 tcaaactcca taagcatgta acttttatct tcttcaatca accccaaaag aggtattggc
      181 aatttatctt ttgagttcaa atatgtgatt tctccaataa ttaaattact gtccaattga
      241 taacgaataa atacaggtat cttttcatct atggttcctg tccagcatat atcactgtat
      301 ctattgatct tagtgtttgg caaagatttt gaagtatcgg aaatagtgtt atcagaactg
      361 ctttcttttt gcggtgaatt gttacaggaa ataattgtca gtaaaattat gcagatcaat
      421 tgtttcattt tagattaatt taattttaag gggaattaaa ggtggtcaaa tataaccaat
      481 atgctgaaat attttcatac aattatcaaa atatctccaa tgtgatatca gaataatttg
      541 attgtgtaaa taaagatgat ttcttattga ggaaattagc aataagctga gtttacagaa
      601 atcatatacc catctccctt tccgaaaccg ctgcaataat aataatcaca gcaatgcaaa
      661 ttagtggaat tacaaacgct gcaagaccca cccaaagata aacaacagca ccgcctattg
      721 ctccgcataa atatataatc cagctaatgc caaagatcca cggatttctc cctgaccaga
      781 aaccgctgtc ctgagaatcg gaattattaa taacgcgcgg aaccggtgtg ccgaaatgat
      841 acaaccacca cataagcttg gtggaaaagg atgtaatcgt tccggtgaca tatgttgtag
      901 ccggaccgga atgaagcatt aatacagcag cgctttgaat tcccattgcg caggcagtca
      961 gagaaatgag gatagtggta tgtatagtcg tttgaaccgg tccaaaaaac agccagaata
     1021 aaattagaat tatcagcaga atcaattctg taaacagagt ccatccgacc ggaccaagcc
     1081 gtgatgaaga tttttttcgg gaaacaatta tcgactcccc aagtaatgct ccggtaatat
     1141 atgctgcaat agaaacagca gagaaatatc cggcggtgaa attcccctga gcgagtgcga
     1201 cagccaggag aatagtgttg ccggtctgtg cggctgtcag aacattaaag ctaagtatga
     1261 taatggcatc cacacttcca cctgcaatag atagcaggaa gatcataatt tcacgactgg
     1321 agattaagtg ttgtttatcg gtaatattct gctcttgatt accctgcaaa atctcagatt
     1381 ttaattcgga aatttagttc gataaaatta tatgtaatta caatacatta gaaatcaatt
     1441 tgattggggg ttttattaat ttattattga ttgtcttaat ttaatttcat taaccggcat
     1501 tctcataagc agttcttatc caatcaataa cttccttatc aatttcttta atgtctgcaa
     1561 ttttaatttt atgtgtacac atggaacccg gtttttcagc tgttaatttt cctttggatt
     1621 cctgtccctt aatgttgatg ccaatttcaa aacgggtttt agtagcaggt tccagtaatg
     1681 caaattgctt ttttcttcgg agacttacat tagcattttt tggtgcgatc tcaatgtcct
     1741 ttccaaattt cattatctcg gaaatgagtt tgtcgtaaat cggtttaaag tgttctttcc
     1801 ctttatattg cttttcaatc aggtcagatt cattttctgc cgaacctgca tcggtgcctc
     1861 tggatttaag tgcaacaaga tttgcaaagc cgtgagttaa accgtgttct tcttttagat
     1921 acttcatgat ctctccgtgt ttggcaaagt cttttttctt cacaatttca atccattgct
     1981 ccagtgtctt acctgaattc ttgtgaaggt tttctatcat tgtttgagtc gctttatcca
     2041 tcttctatat tctttaatta ttaattaatt ttgaaatttt tctcaattta agtcattttt
     2101 ataattccgt tgtttatcta tttctaaatt tttccatatc cagaaaaata ataccccagg
     2161 tatgaccatc ataatcctcg attgttcgga cctgcataaa accttcatcg atcactggaa
     2221 ttgattcggt accacctgct tttattccgc tttccataat ttcatttacc ttatcaagac
     2281 tttcgacagg aagcgtatat gatgcggaca tatagtcctt tgtatccgga attttttttc
     2341 tattataatg taagaacatt ttctgcgtca taagcataac ataaatgtga tcgctccaga
     2401 ccatacactt ctggtcttca aatgtaaatt cgggattgat cgtaaaacct aactgagtgt
     2461 agaagttcat tgatctctca acgtctgcta caggaagatt gataaatatc tgcttcattt
     2521 tttttttaaa tattagttta aattattttt cgtactatat aatataattt tcagattcat
     2581 gatttttcag ataaaaaaaa tattaaatcc gaataataat aacaatactg aaaacaaatt
     2641 gaacttattt atcacatgtt taccaagcat tggacagatc aattctatat tgtaataaat
     2701 taaaggatgt tgtacaatct gaagttgtct gagataattt ctgataagga gaatagttga
     2761 ctattattct aattaataag agaaattaat tccggataat ttttcaaata attcgatttg
     2821 agtcctacgg atgaaaactt tatataattc cgaattaaat ttataatatg catttttgta
     2881 cagttaaatt tttaaaccaa aataatttct taaaatgaaa atattattat ccttactgac
     2941 atcattgatg ctgatgatct tttttaacca aaatactttc tcacaacaaa cagataactg
     3001 gcagtggctt aatccgaaac ctcagggaaa tactatctat gctttggatt ttgtaaataa
     3061 cagcaccgga tatgcaggcg gtgattttgg tacaatcatg aaaaccactg ataacggcga
     3121 tacctggaca aaacttaata ccggattttc tgatagactg ttaagtctag atttcattga
     3181 tgacaataca ggatttgccg gcggtgatga tcagctctta aagaaaacta cagacggagg
     3241 tcagacctgg actgatgttt cgcttccttc tgctccggac ttttacatct ttgacataga
     3301 ttttgtaaat tcccagaccg gatatgtact tggatttttt gtaatggaaa gtcttatatg
     3361 gaaaacaact gacggcggtc ttagctggac gactcagact actgacgggg caaattattt
     3421 aaacaatctt tatttccttg atgaaaataa tggatttgct tcaggcggta ctttaggagg
     3481 tcaaattata aaaaccacta acggcggact aaactgggat tataattatc aggacaatta
     3541 tgcaaaacac agtatggtat ttctcaattc tctgacagga tattccggaa gtgatcaggg
     3601 aagaatttat aaaactattg acggtggaaa tacatggaat tttgtattgt ctgacggagg
     3661 aattgatatc atgtcaatga attttacaga tgcaaataca ggatttggat ttggaaccgg
     3721 ttcggtgtat gtgaaaacaa cagacggcgg agacaactgg tatcagagta atcagatcgg
     3781 aagcagtatg aacagccagt attttgatgc atcgataaca ccgaacggat caattcatgc
     3841 tgcaggaaca ttcggtgcaa tggtcagatc aactaacagc ggcgcggatt tcacatataa
     3901 agtatctgta actgacggat atgtatctga cattgaattt ataaatatta acacgggcta
     3961 tgctgtcacc ggttataatc acggcgatat tttaaaaaca actgatgctg gtgccagctg
     4021 ggtttctcag attacaactt acacgactcc aatatacggc atttctttta caagtaatga
     4081 aacaggatat cttgccggaa gtatttcact ttataaaact accaatggag gaacaaactg
     4141 gaacacagtt tataacagca caacaaatga aatattcaca gacattgttt ttacaaatgc
     4201 aaatacaggc tatgctgtcg gaagctatgg aaggcaaatg aaaacaacta atgcaggatt
     4261 aaactggact gcatctacaa tttcaagctc aggttcaata ttgagttcac tttgttttgt
     4321 aaatgaaact accggttatg cagtaggtga taataatgct gcagtccgta ctacaaattc
     4381 aggtcttaac tggaattcaa tgtcggttgc atctccgttt ataaatttaa cggatgtatt
     4441 tttcactgat gttaataacg gttatatttc atgcactaca ggaatttacc gaactacaaa
     4501 cggtggtgat tcatggtttg ctttatcgac cccggctggt ggatatgcaa aagttcagtt
     4561 cagaggaaac ttcggctatt caattacagg taacggtaag ataattaaga ccatagatgg
     4621 gggagtgaac tggatagtac agccgaccat tactacaaac ggactttcag cgctttactt
     4681 taattcagat aattttattt atgccggagg tttactggga acgatgctta aaactattcc
     4741 tactgaacta atcattactc atgcgggtaa tattaatacg gaaattccgg gaaatttttt
     4801 actctcacag aattatccga atccttttaa tccgtcaact aaaataacat ttgaaattcc
     4861 aaattcagga ttggtaactt tgaaagtatt tgacataacc ggaaagcttg ttgatgtatt
     4921 gttaaacgga aataaaaaca gcggaaaata tactgtagat tttaacggaa gttctttagc
     4981 aagcggtgtt tatttttaca gtctgacatc aaatgatttc acggatacaa aaaaaatgat
     5041 actgatgaaa taatttttaa attcagctaa atttaaaata ttttttgcaa aaccatttta
     5101 aataattttg cagttgtttg agtctcaaaa aaatctgaag agtttattgt cagttaaaaa
     5161 ttttgaagtg cttcaatctt atttggtttc ttccataaaa gcaacttaca gatagtgctg
     5221 tttatctcat tgttttatat actgctgtat gcctgttcct gcaggccttt gctgacttta
     5281 agcagtttta atatttttta tgtcagtctg taaataaatt cttcagtctc ttcttgccgt
     5341 gcatttgcaa aacctttgtc tgttccaatt tttacaaacc cgcatttttc taaaactttt
     5401 tgcgaaccaa aattgtcaaa tgcaacgcgt gcaaaaattg gtctggtatt ttctaaagtc
     5461 agaaagattt taagtgcagt tgttgcaata ccttttcccc aaaagctctt gtctatccag
     5521 taagttattt cagcatcacc ttccattaca aattttgcaa tacttcctgc tatagtttca
     5581 ccaaacagaa ttgtcatatt atttattgcc gggtcattaa aatgttttgt gtatttttca
     5641 aaatatgctg ctttgtcggc aggatctttt gaagtaaatg cagctaagta atttgcctct
     5701 tcgtcaagct gaaattgaaa gaaaaattct aagtctgata tttcggattg tcttaatttt
     5761 actttattaa tttgcataca ataattcagt taaaaattta aaaaatgtat tccgtagttg
     5821 tgttagttcc aaaaaagtct gaagaattca aattactttt taaatttgga agtaattcac
     5881 tcttaatcag atttctatca ggtatagacc ggattatctc tgaaaagaaa acagttctgc
     5941 attcgcattc ctcttacttt taatcagatc acaaattatt tcggctgcta ttacactgaa
     6001 agttattccg tttcctccaa agccaagagc ataataagta tgaggtgttt tttcataagt
     6061 tccaatataa ggtaaagagt cttttgtagt tccgaaagtg cccgtccatc tgaattccgg
     6121 taagagctca atttcaggaa atagtttttc aaaatctttg actaactggg tagatttttt
     6181 cttgataagt ttatcacgtt ttacagcatt agaaaaatct tcatctcttc ctccgattat
     6241 aacccggtta tcctttgtta atctcaaata taaatacggg tcatctgtat tccatatcag
     6301 aattttatca ttccagactg gtgatggagt tttgatattt tccgaaataa gagcgtatgt
     6361 agaaatcagc tttacgattt ttttatttat aaattctgtt atctcaaaac ctgtagcatt
     6421 gacaattttt tttgcagtta tgttacagtt gttttctgtt cttaatttaa cgcttcgttt
     6481 tttatattcg atctcagtta tatttgtcct gtcatagatt cgcaatcctt ttttttcact
     6541 ttcctttaac agggcatgtg taaataaata agcatctgta gttccgccct gttctgaaag
     6601 tattgcagat gaagcagaaa agccatatcc tttttctata tcattttcaa ttaaatgctc
     6661 aacctgaaat ccacactttt tacggatcaa atattcttca tgaatcagtt catcatgttt
     6721 tttatacgca gcatagaaca ggctattgtt tttttcaaat tctttaaatt tcagtttatt
     6781 agaaatgtca ttcaatgtgt ctatcgactc actgcatagt ttgtaagacc tggcagcttt
     6841 tttaaaacct atttgctttg atagtttgca aagaggagta tcgagttcat attgtaaaag
     6901 tgatgtgctt gcgcaggtgc ttcctaaacc tattgtcctg gcatcaacta aaatgcagtc
     6961 tattccggaa ttgatcagat aataagatgt cagcgcacct gatatcccgc cgcctaaaat
     7021 taccacatca gtttttacat cctgataaag tttttcaaat ttatttggaa gaccactgtt
     7081 gatcagccag tatggatagc cacttgttaa attcatttat gctgtagttt gaaaattatg
     7141 aaaagaattt attaaatccg gcagatctat taatcaacta atttaaattt aataatgaat
     7201 aaaatcaaaa cactttgatc aattggaatt ttaggcaagc tgaatttcaa taattcattt
     7261 cctgtttact gacgtccttg agggaagttt gtatgaaatt cttgttgatg aattgaaagg
     7321 atttggataa ttttgatata aataaaaatc gctttcaatt acgaactgat tatttatgag
     7381 tgaagtcagt attcctctat tagttagttg ttcttaaaaa gttgttctta aaatgcatcc
     7441 accaggacta accgcacttc aaagtcatac tactttctta atatcttttc cattgccttt
     7501 ccttttgcaa gttcatcgac cagcttatct aaataccgga ccttttgcat aagcttgtct
     7561 tcaatttctt ccacacgata gccgcaaatt acaccggtaa tttttgatgc atttttattt
     7621 aattttgcct gagcaaaaaa agtctcaaaa tcagttctgt tgtcgagatg attttgtaat
     7681 tcttttttgt catatccggt cagccagcaa gtcactgtat ctacctcttc ctttgtccga
     7741 cctttttttt cagctttagt aatatagtga ggatacacac ttgccagtga cattttatat
     7801 attctgtgtt tttccataat tctgaaatat ttttataagt gaaaaattta gtaaatagtt
     7861 tttaaaacaa aattgatttg atccaaattt taaaattaga atgcattatt tgtttatata
     7921 tatttttttc ctctcataca gctttaagga atatttttgt acatcaggta atccttttat
     7981 ctttaattgc cttaacccat ttacattcac tgcaaatatt acagacaggg agcgacccgg
     8041 tttgcagcac ctttgtattg ctgcaatcga tacaggaata ataaccttcg actggaatgg
     8101 aattcagtgg atgttcaata tactcaggta ttatcggcaa gccatatttt acttcttcat
     8161 cttcaaaaaa gaatacactc aactgaccgt cctcctccag tatagctctg tgaacctg
//
LOCUS       DAORVQ010000149         8213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4983_length_8213_cov_4.777274, whole genome shotgun sequence.
ACCESSION   DAORVQ010000149 DAORVQ010000000
VERSION     DAORVQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_8213_cov_4.777274"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLX80_11065"
     CDS             complement(<1..1033)
                     /locus_tag="PLX80_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21372.1"
                     /translation="MIKNLFTLFIFSTAIFFHSTDLFSQYSSPESVTYDSIGKRYLVS
                     NTQGQKISQRSQTGVVTDFVTVGGSIHGVTVYGNNVYVCNGTRIKGYDLSTAAEIFNV
                     TLTGSTFLNDLAADENGILYVTDFSTRRIYKVNTANSEWWIYVANTTNTPNGVYVDEF
                     RNRLLVCCWGSSAPVKQVNLADSSISNIISTPYSNCDGISLDKFDNVYISTWGIQSVV
                     RYDINFSLPAVVVANGLSNPADIYVNKNTDTLAIPNAGNNTVVFQLLEYPLGINSNET
                     TNPESIALYQNYPNPFNPKTVINYELGVTGNAMLKVFDVLGNEVAELVNEKQNAGRYS
                     VEFDGNGFAS"
     gene            complement(1109..2251)
                     /locus_tag="PLX80_11070"
     CDS             complement(1109..2251)
                     /locus_tag="PLX80_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21373.1"
                     /translation="MKKIFLCILIFISCNAFAQFSNNGLDFLTPANFNYPVRQQLWIL
                     TGDDPFVNEKDIFKIELSYNYSSAEKYFDPAGETHEIKKSYFQYNKGSDQGGYFRKTG
                     AILTGQYHWKGLNKVIIRFPFTFTEMKSYSSTTDVKPQEEFIKPRGPFQDLELSYERK
                     VSLSDKFSFYAGGGLTIPSSRPKRYLDNPQGGYGNTWTNNLNMFLSYAISDWRFTGGA
                     KYIITYPHTEQLFTPRPYGIGYPFQYDTTSITYNALNAYIEQNPYEATIKAGSLVFAD
                     FVADYFFKFGLDASLQFQYFYSSGDKFDQSIPVKMARVNGSIEPVGYLSELEGGYSGT
                     IRLYLTQDFAKISKSNFSFTLGFGSSVFGSNAQGEANIILGLIGIF"
     gene            complement(2280..4061)
                     /locus_tag="PLX80_11075"
     CDS             complement(2280..4061)
                     /locus_tag="PLX80_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQY21374.1"
                     /translation="MKIYINFFKTLLFCFTFISVTGNVCIAGPDDELNQIFADRVEYN
                     LRTYPEGATYDGDHRYDDMLSDNSEAANIEYYDSARAYIARLKSIDYNSLSESNKLNY
                     DLFLSSLEEDLEGEKFNWYLMPLGQQSGIHIGAPQIVNVQPVTTYEEYQKYFSRLRQL
                     ESKFNNTIDNMRKGMSQGYVMPVFLMDQTLPQMENIMNKEIEKSIFYSVVEKDNKMSD
                     EEMELAKKELKEIIATVINPSYQKLYDFVKNEYLPVCRIDAGIWAVPNGKERYEYSIK
                     SYTTLDLTADEIHNIGLSEVSRISAEMERVKDETGFKGTLSEFNDFLKTDTQFYFTDK
                     EDLLKGYRDILTNMDAKLPELFGTLPEADYDLFEMEEYRAASAPQAYYYSAPEDRSRP
                     GLFYVNTFNLPSRPKYTMTALALHEAVPGHHLQISIAQELKGLPKFRRDYSVTAFVEG
                     WGLYAESLGYETEMYEDLYQLYGALTFEIWRACRLVVDTGIHDKKWTREEAFEFMKKY
                     TPNSDHDIQTEIDRYISWNGQALAYKIGELKIKELRKTAETRLGENFNVKDFHDQVLK
                     NGAIPLPLLEKIIDEWIESQLIKNELN"
     gene            complement(4277..6496)
                     /locus_tag="PLX80_11080"
     CDS             complement(4277..6496)
                     /locus_tag="PLX80_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21375.1"
                     /translation="MDLTLILTFIGIALSILIPVVGYFYKNHKDMSNYYSLIWKSSKK
                     ILPKDLLNERPFEDFYFEREVDLFLKKVLSEKKNAMLTGPPLSGKTRAFYNAVKKYNG
                     KFYILATRSILMSHFLLPAYYRYNKNRLIFIDDFQNFVEKQESFPQLFKTAKAKDIPV
                     IVTCHSGKEFTKARNKLIENNLDADILFGENIFEIGKVSHEEGKIIADRMGRQWEHIS
                     FNGTIGSIFMKLNEMQRRFEQSGTIEKTILRSMRSLYVCGVYEDNTYFKMDRIKKVAG
                     QSELEGKDFEWTGWIKSLEEKEFVHLSKKNLVWAEDAYLEYIVKPETEISNVELFEEI
                     IEIFKDDAEALQMAGERAYDNGSVSIEILKYLRLSINAFERVYELADNINDRQKIFSA
                     LRYLGMSYWSLSKLENAKENSLKSINYFNKIFSRSDLDLQPSESALIKNRIGNSHSVL
                     AESENKRENCLKAIDLFKEALNIYSKDEYPLEYAQVKNSLGAAYLMLSDDEDAKLNLS
                     KAAECFEDSLKLRTAEIDPRGFAYSKVNLANTYARLSQIENGALNLGTALNLYSDTLK
                     IYLKEKYPNQYGMAHNNIGNVYFLLAGISDKKLNYRKSMESYMKALEVRSSENTPVLY
                     SNTLYNLSDTYLMLGVTESDPEYFKKALSCLNELLNIPASNLTPFRTGEIYYTTGRSY
                     SLLAELESGQANYEKGIEFYEKSKEIFKDNDFNDMYNKTNEELEKSKKALEKISENL"
     gene            7085..8029
                     /gene="miaA"
                     /locus_tag="PLX80_11085"
     CDS             7085..8029
                     /gene="miaA"
                     /locus_tag="PLX80_11085"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQY21376.1"
                     /translation="MKKVLAVVGPTASGKTSLSIKVAGKINGEIISADSRQVYKNIPI
                     ATAQPSEDDLKIVKHYFINELELSDEFSAGQFGIKGREIISDIFSRGKLPVITGGSGL
                     YIRSIIDGLFENDSKSDEIREKLYSEYEKHGEEYLYNELYNVDPVTFEKIPKGKHRRV
                     IRALEVYYTTGKKISELQNELPEIDFKTVQIGLNHDRKILYERINNRVDAMLSNGLTD
                     EVKKLLESGYHFKNNYSVDTVGIKEVIKFLEGEYDLEYMKEMIKQNSRRYAKRQMTWF
                     RKDKRIHWINMEEHSEEEVVNLVLEIFKSEKFIPLFNN"
BASE COUNT         2694 a         1429 c         1224 g         2866 t
ORIGIN      
        1 cgctcgcaaa accatttcca tcaaactcaa ctgaatatct accggcattc tgcttctcat
       61 tgaccaactc agcgacttca tttcccaaaa catcaaatac ttttagcatc gcattacccg
      121 taactcctaa ctcgtaatta ataactgttt taggattaaa tggatttggg taattctgat
      181 aaagggcaat gctttcagga ttagtagttt cattcgaatt tattcctaaa ggatattcca
      241 gcaactgaaa cactaccgta ttatttccag catttggaat tgcaagtgta tcagtgtttt
      301 tatttacata aatatcagcg ggattactga gtccgtttgc aactacaacc gccggaagtg
      361 aaaaatttat atcatatctg actacagact gaattcccca tgttgaaatg tatacattat
      421 caaatttatc taaagaaatt ccgtcacagt tactataagg agtagaaatg atatttgaaa
      481 ttgaagagtc tgcaagattt acctgcttca ccggtgcgct tgatccccag cagcaaacca
      541 gcaatctgtt tctgaactca tcgacataaa caccattcgg agtgttagtt gtgttcgcta
      601 catagatcca ccattcactg tttgctgtat ttactttgta gatccttctt gtagaaaaat
      661 cagttacgta aagtattcca ttttcatcag cagcaagatc attcagaaaa gtcgaacctg
      721 ttaaagttac attaaatatt tcagcagcag ttgaaaggtc atatcccttg attcttgtac
      781 cgttgcagac atagacatta tttccataaa cagtaactcc atgtatcgat ccgccgacag
      841 ttacaaaatc tgtgacaact cctgtctgac ttctttgtga aattttctga ccctgtgtat
      901 ttgaaacgag atatcttttt ccaatgctgt cataagttac actttcagga ctgctgtact
      961 gtgaaaaaag atcggtgctg tgaaaaaata ttgccgtgga aaaaataaat agtgtgaata
     1021 aatttttgat caatttgatt tattttattt ttgaataatt ttttaaattt tgaatcatat
     1081 aatacttaat ataatgaaac atagataatc agaaaattcc gataagaccg agtattatgt
     1141 ttgcttcacc ctgtgcatta cttccaaata cggatgaacc aaatccaagt gtaaagctga
     1201 aattgctttt agaaatctta gcaaaatcct gagtaagata caatctgatc gttccggaat
     1261 atccgccttc aagctctgat aaataaccta ccggttctat cgagccgttt actcttgcca
     1321 ttttaacagg tatgctctga tcaaatttat ctcccgagct gtaaaaatac tgaaactgta
     1381 atgatgcatc taatccgaat ttaaaaaagt aatcagctac aaaatctgca aatacaagtg
     1441 aacctgcttt tatagttgct tcataaggat tttgttcaat gtaagcattc aaagcattat
     1501 aagtaattga cgtagtatca tactgaaaag gatatccgat tccatagggt ctgggagtaa
     1561 ataactgttc agtatgcgga taagttatga tatatttagc cccgcccgta aatctccaat
     1621 cactgattgc gtaagacaaa aacatgttca aattatttgt ccaggtattt ccgtaaccgc
     1681 cctgcggatt atcaagatat ctctttggac gtgaagacgg aattgtcagt cccccgccgg
     1741 cataaaaact gaacttgtct gacagagata cttttctttc ataagataat tccagatcct
     1801 gaaacggacc gcgtggcttt atgaattcct cctgaggctt tacatctgta gtggaagaat
     1861 aagatttcat ttcagtgaat gtgaacggaa atctgatgat aactttattt aatcctttcc
     1921 agtgatattg tcctgtaaga attgctcccg tctttctgaa atatcctccc tgatcgcttc
     1981 ccttattata ctggaaatat gattttttta tctcatgagt ctctcccgca ggatcaaaat
     2041 atttttctgc ggaagaataa ttataagaaa gctcaatctt aaaaatatcc ttttcattca
     2101 caaaaggatc atcacctgtc agtatccata actgttgtct taccggataa ttaaaattcg
     2161 caggtgtaag aaaatccaga ccattgttgc tgaactgtgc aaaagcattg cagcttataa
     2221 aaatcaaaat acataaaaat atttttttca tcagtgttta atttgattaa caaaaaattt
     2281 taattcagtt catttttgat cagttgagat tctatccatt catctataat tttttccagt
     2341 aaaggaagag gaatagcacc attctttaat acctgatcat gaaaatcttt tacattaaaa
     2401 ttttcgccta atctagtttc agcagttttt cttagttctt ttatttttag ctcaccgatc
     2461 ttataagcca gtgcttgacc attccatgaa atatatctgt cgatctctgt ctgaatgtca
     2521 tgatcggaat ttggagtata ttttttcata aactcaaacg cttcttcccg tgtccatttt
     2581 ttatcatgaa ttcctgtatc aactactaat ctgcaagctc tccatatttc aaaagtcaac
     2641 gcaccataaa gctggtatag atcttcatac atttctgttt cgtatccaag actctcagca
     2701 taaagtcccc agccctcgac aaatgcagta acgctataat ctcttctgaa ttttgggagt
     2761 cctttaagct cctgagcaat tgagatctgc agatgatgtc cggggacagc ttcatgaagt
     2821 gcaagcgctg tcatggtata ttttggtctc gaaggaagat taaatgtatt cacataaaat
     2881 aatccgggcc tcgatctgtc ttcaggagcg ctgtaataat atgcctgcgg tgctgacgct
     2941 gctctgtatt cttccatttc aaaaagatca taatctgctt cgggaagtgt accgaataat
     3001 tcaggaagtt ttgcatccat atttgtaagt atatctctgt atccctttaa tagatcttcc
     3061 ttatcagtaa aataaaactg agtatccgtt ttcagaaaat cattaaattc tgacagtgta
     3121 cctttaaacc cggtctcgtc ttttactctt tccatttctg ctgaaattct gctaacttca
     3181 gaaagaccaa tgttatggat ctcatctgct gtaagatcaa gtgtggtata actttttatg
     3241 gaatattcat atctttcctt accgttggga acagcccaga tccctgcatc aattcggcat
     3301 acaggtaaat attcattttt tacaaaatca tataattttt gatatgaagg attaattacc
     3361 gttgcaatta tttctttaag ctcttttttt gcaagctcca tttcctcatc ggacatttta
     3421 ttatcctttt ctacaacgga gtaaaatata gatttttcaa tttccttatt cataatattt
     3481 tccatttgag gcagtgtctg atccatcaga aatacaggca ttacatatcc ctgagacata
     3541 ccctttctca tattatcaat cgtattattg aatttacttt caagctgtct tagtctggaa
     3601 aaatatttct gatattcttc ataagtagta accggctgca catttactat ttgaggtgca
     3661 ccaatatgaa taccactttg ctgaccaagc ggcatcagat accagttaaa tttttcacct
     3721 tccaaatctt cttcaagtga tgacaggaaa agatcataat taagtttatt gctttcggaa
     3781 agactattat aatcaatcga tttcaacctt gctatataag cccttgcgga atcataatat
     3841 tcaatgtttg cagcttcaga attatccgaa agcatatcat catatctgtg atcgccgtcg
     3901 tatgtagcac cttcgggata tgtgcggaga ttatactcga cccggtcagc aaatatctga
     3961 ttaagttcat catccggtcc ggcaatacaa acattacctg taactgaaat aaatgtaaaa
     4021 caaaagagta gagttttgaa aaaattaata tagattttca tattattata aaataattta
     4081 tcgaattaat ttattatttt tttagaatta aaaagtttac caaaaataaa aaaatctgtc
     4141 cataataact aaatactatt ttaaggaaat tacggaacaa aaattcgtgg cacagattat
     4201 taatctgtgt caagctgatt cggaaatatg gagaacagat tcgtaacagg ttatcaatct
     4261 gtgtaatgga gttatactat aaattttcac tgatcttttc cagagctttc tttgactttt
     4321 caagctcctc atttgtttta ttatacatat cattaaaatc attgtcctta aatatttcct
     4381 ttgacttctc ataaaattca ataccctttt cataatttgc ctgtccggat tcaagttctg
     4441 caagcagtga ataacttcta cctgttgtat aataaatttc gcctgttctg aatggtgtaa
     4501 gatttgaagc aggaatattc agcagttcat tcagacagct taatgccttt ttaaaatatt
     4561 caggatcact ttctgttact ccaagcatca gatatgtatc acttaaatta tataaagtat
     4621 ttgaataaag gacaggtgta ttttcagatg acctaacttc cagagctttc atgtaagact
     4681 ccatagattt ccggtagttt aattttttat ccgagatgcc tgccagtaga aaataaacat
     4741 tgcctatatt attgtgagcc attccgtatt gatttggata tttttccttt aaataaattt
     4801 tcaatgtatc gctgtaaaga tttaatgcag ttccaagatt aagtgctccg ttttcaattt
     4861 gtgaaagcct tgcgtaagta ttcgctaagt tgacctttga atatgcgaat cctctcggat
     4921 caatttcggc agtccttaac ttgagtgaat cttcaaagca ttctgcagct tttgataaat
     4981 ttaattttgc atcctcatca tcactaagca taagataagc agcaccaaga ctatttttta
     5041 cctgtgcata ttctaacgga tattcatctt tagaatatat attcagagct tctttaaaaa
     5101 gatcaattgc cttgagacaa ttttctcttt tattttcaga ttccgcaagt actgagtgag
     5161 aatttccaat tctatttttt ataagtgcag attctgaagg ctgcagatca agatccgatc
     5221 tgctaaatat tttattaaaa taatttatag acttcagact gttttcctta gcattttcca
     5281 gtttggataa agaccagtat gacattccaa gatatctgag cgcactgaat attttttgtc
     5341 tgtcattaat attatcagcc agttcataaa ctctttcaaa tgcattgata gaaagtctta
     5401 aatactttaa aatttctata cttacacttc cgttatcata agctctttct ccagccatct
     5461 gcagcgcttc tgcatcatct ttaaaaatct ctattatttc ttcaaacaat tcgacattcg
     5521 aaatttcagt ctcaggtttt acaatatatt caaggtaagc atcttcagcc cagactaaat
     5581 ttttctttga gagatgaaca aattcttttt cttcaagtga ctttatccag cccgtccatt
     5641 caaaatcctt tccttccagt tcagactgcc ctgctacctt ctttatccga tccattttaa
     5701 aataagtgtt atcttcatac accccgcata catacagact tctcattgat cttaaaattg
     5761 ttttttcaat tgtgcctgac tgctcgaatc ttctctgcat ttcattaagt ttcatgaata
     5821 tagatccgat agttccatta aaactaatgt gttcccattg ccgtcccatc ctgtctgcaa
     5881 ttattttccc ttcctcatgt gagacctttc caatttcaaa aatattttca ccgaataata
     5941 tatctgcatc gagattattc tctatcagct tgtttcttgc ttttgtaaat tcttttcctg
     6001 aatgacatgt aactattaca ggaatgtcct ttgcctttgc agtcttaaac aattgaggaa
     6061 aactttcctg cttctctaca aaattctgaa agtcatcaat gaaaattaat ctgttcttgt
     6121 tatatctata atatgcagga agtaaaaaat gactcattaa aatacttcgt gttgcaagta
     6181 tgtaaaattt tccgttgtat ttttttactg cattataaaa tgctctggtc tttccactta
     6241 atggcggacc tgttaacatc gcatttttct tttccgacag gactttttta agaaataagt
     6301 ccacttccct ttcgaaataa aaatcttcaa agggtctctc atttaacaga tcttttggta
     6361 aaattttctt tgaacttttc cagataagag aataataatt actcatatct ttatggtttt
     6421 tataaaagta tccgactaca ggaattagta tggaaagtgc aattccaata aatgttagaa
     6481 ttaatgtcag gtccaatgag gtgatttatt tttcaaaaat atataaaggt caggagaatt
     6541 gaaatgaagt ttttgtttgt gaggatttaa ttaagttttt catttttatg attaatttat
     6601 aaatttaagg ttgaacaaag cttcgcttgc agaagctttg tttatcaact ttccatcatc
     6661 cctgacaatt aaaaataaaa aatgaataat tgaaaaatct tcttcaggga tgatgaaaat
     6721 aattttttta attgatcaaa aaaaaacatt cagatctaat ttccaaaatc aaattatcct
     6781 tttaaaatta gattaaaatg tcatttgatt tatgaagttt tcagtaacat gaataatggc
     6841 ataatttttc aatctcatat tttaatacat ttataaggaa tatctttata gatataatat
     6901 tgtctcataa attctactgc cctatattca aaaaaataaa catatttatt tctacttatt
     6961 ttacattact aaagtgcagt ttatttttaa taaattgatt taaataaaaa ttaatgcttt
     7021 caattttgta agttttaatt aataaaattt tctataattt agtttttaaa ttttgttact
     7081 taacatgaaa aaagtacttg ctgtcgtagg acccactgca tccggtaaaa cttctctatc
     7141 tataaaagta gccggaaaaa ttaatggtga aattatttct gctgattcga gacaggtata
     7201 taaaaatatt ccgattgcaa ctgcacaacc gtctgaggat gatcttaaaa tagtaaaaca
     7261 ttattttata aatgaacttg aactttcaga tgaatttagt gcaggtcagt ttgggataaa
     7321 aggcagagaa ataatatctg atatttttag cagaggaaaa ttaccggtaa ttacaggagg
     7381 gagcggactt tatataagat caataattga cggcttattt gaaaatgatt caaagtctga
     7441 tgagatcaga gaaaaattat attctgaata tgaaaaacac ggagaagaat atttatataa
     7501 tgaattatat aatgttgacc ctgtaacatt tgaaaaaatt ccaaaaggca aacacagaag
     7561 agtgatcaga gcattggaag tttattatac aacaggaaaa aaaatatctg aactgcagaa
     7621 tgaattgccc gaaattgatt tcaaaactgt tcagattgga ttaaatcatg acagaaaaat
     7681 tttatatgaa agaataaata acagagttga tgcaatgtta tcgaatggat taactgatga
     7741 agtaaaaaaa ttacttgaat caggatatca ttttaaaaat aattattctg tagataccgt
     7801 tgggataaaa gaagttataa aatttttaga aggagaatat gatttggaat atatgaagga
     7861 aatgatcaaa caaaacagta gacgatatgc aaagcgccag atgacctggt ttagaaaaga
     7921 taaaagaatt cattggataa atatggaaga acattctgag gaggaagttg taaatttagt
     7981 tttggaaata tttaaatctg agaaattcat tcccctattt aataattaaa aattcatgga
     8041 aatttatttc tacaaagaaa gttaaaataa atttctatat attgttcaca ctctaaaccc
     8101 ccaaattatt accccaaaga aaaatgattt gaaaagtatt ttattcattt tatatttttg
     8161 agtaaaatat ttatttatta aacaaaaaaa tttattaagt atgaaaactt ttt
//
LOCUS       DAORVQ010000150         8205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_4993_length_8205_cov_5.681595, whole genome shotgun sequence.
ACCESSION   DAORVQ010000150 DAORVQ010000000
VERSION     DAORVQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4993_length_8205_cov_5.681595"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLX80_11090"
     CDS             <1..881
                     /locus_tag="PLX80_11090"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQY21377.1"
                     /translation="ESLDKWNIGDSAFDFLYLGKNDIDYKLPGTLNKVFDHEYWVSAD
                     QSDIINDSYPLLTVDEYLSEPVVSDKFNFLEITLDDLDKELLEKLSLEKNFILILSTD
                     NPNTMSELRRAFIELINNNVELPVIIKRDFSNNKDLTDDELLVHTSTDIGGLLLDGLG
                     DGVWIESSGTEIKKINNISFGILQATRTRITRTDYISCPSCGRTLFDLQETTAKIRKR
                     TDHLKGMKIAIMGCIVNGPGEMADADYGYVGSGPGKITLYKGREVVKKSIDTEIAVDE
                     LITLIKENNDWTEVSN"
     gene            924..2519
                     /locus_tag="PLX80_11095"
     CDS             924..2519
                     /locus_tag="PLX80_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21378.1"
                     /translation="MNPIKFFRRLAIILIIISSGFLKADEPYTIQLEQVNIPGSPKIH
                     SFAFADDNGKWLFIGGRINGMHGFDAATAFPKQFSNTNIFVVDPSTNMTYSRNLFTDL
                     NFTVADPLRSTNMQYYYDGTKLYITGGYGFDSTFNDLLTFPTLTVVDVSQTIQAVING
                     TSVAPYIRQISDPRMKVCGGEMQKLGDYFYLAGGHIFTGAYSPISNDQVYTNEIRKFK
                     INDNGTNVSISDYSASEDTIEFHRRDMNLVPAIKPDGVSQYITLYGGVFRSYADLPFM
                     NPIYIDENNAVTDFGFEQKMSQYTCSYLSAFNSANGSMHTTFLGGTSLYYFNEVLNQQ
                     IRDSLVPFINDITTLTRNSNGTSIENISPTKMPALLGTNAKFILSPSIPQYSNKVIKL
                     NEISGNTFAGYIFGGIRAYLPNNTPSFASEYILKVYINPEPIGIQQISGNVPDKFSLG
                     QNYPNPFNPVTKIKFEISKNDFVRLAVFNSIGKEVAVIVNENLSTGIYETEFSAEGLS
                     SGIYYYKLTSDNFSETKKMLLIK"
     gene            2809..4680
                     /locus_tag="PLX80_11100"
     CDS             2809..4680
                     /locus_tag="PLX80_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21379.1"
                     /translation="MRIRLLITLLFVSQFTGLIKADTEPYTVDLEQIFIPNAPAIHSF
                     AFAESNGRWLFIGGRTNGLHGFNAVNSFPKQFSNIYIFVVDPLTNLTYARSIFTDLPF
                     AFADQLRSANMQSFQDGNKLCIIGGYGYDSTANGLVTFPSLKIFDVEETINAVMNGNS
                     VAPYVRGFTDIRLQVCGGELMKLGDYFYLVGGHKFTGTYRLNVNNQVYTDQIKKFKIN
                     DDGINVSITNYTEFTDTAEYHRRDMNLIPAIKPDGVTQYLTLYGGVFKKNADLPYLNP
                     IYIDENSITVDYSFEQKLSQYTAAHLNAFNSMTGSMHTTFFAGTSLYSVNDTTGVLEY
                     DPLVPFINDISTLTKSSNGISQEIVNAEKMPALLGTNAKFILNKSVPHFDNEVIKLDQ
                     LNYTRTFVGYIYGGINAQLPNNGPSTPSDYIFKVYITPDFPLPVELNSFTSEISGRDV
                     KLKWSTSSEINNSGFYIERKTASVVNSSWENISFQEGNGNSNSVNNYNFTEKNMNAGR
                     YTYRLKQIDFNGSFEYYNLSGDVVIASPNNYSLHQNYPNPFNPSTKIDFEIPESGTVS
                     LKIYDILGNEVMTLINENKQAGFYSIEFKGSEFSSGVYYYKLESKGFTASRKMLLVK"
     gene            4690..5658
                     /locus_tag="PLX80_11105"
     CDS             4690..5658
                     /locus_tag="PLX80_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY21380.1"
                     /translation="MVNNNTLKILSISIFIFTVNSCQTFIDVGDNFVRQLSSPEKVIK
                     KVKDPIRKDVKLSALWIGHASFLLQIYDKVILIDPVFTNNVAQVLRRYAEPGLDLNSL
                     KKIDMVLISHSHMDHLSLGSLSDIEDKFHGTDIVFPAGVENYLPDYDMNFIKMNFTDY
                     RKKFTGETKLIDSVKITAVPAVHWGGRYGLDGKFFSSNGYCGFIIEYRDVTVYYTSDT
                     SYEPDLFKYIGDNYDIDLVLVNIIYCKGCTEINQGTSHILPMGAIKILDDTKAEYMIP
                     AHFGMFTDPNVQIRELKKMYLTNDEYKRKVKILNIGEQFVIKSSEK"
     gene            5788..6825
                     /locus_tag="PLX80_11110"
     CDS             5788..6825
                     /locus_tag="PLX80_11110"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HQY21381.1"
                     /translation="MKTKKDYLKETIQHIDIKKHNVIPLVDDMRNMAFSSRELATAAD
                     YYDMMLADKNCSVMLCLAGSLFSAGLKNVVVDMVRNNMVDVIVSTGAIITDQDFFEGL
                     GFKHYKGSQFVDDNEMRELMIDRIYDTYIDEEDLRVCDYTISQICDTLERRPYSSREF
                     IWHMGKYLSENGKNNDSVVLAAYENNVPIFVPAFSDCSAGFGFVHHQHKNPDSHVSLD
                     SAKDFLELTKIKLAANESGLYMIGGGVPKNFAQDTVVAAEILDKEVPMHKYAIQITVA
                     DDRDGALSGSTLKEASSWGKVDMTYEQMVYCEATIAMPLITGYAYHKGNWKNREKREF
                     QKMFENEKAEA"
     gene            complement(6896..7384)
                     /locus_tag="PLX80_11115"
     CDS             complement(6896..7384)
                     /locus_tag="PLX80_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY21382.1"
                     /translation="MKSDYKIPQQTRIGHVHLKVSDLERSLEFYSGLLGFEVTVKYGL
                     QAAFISAGGYHHHIGLNTWFSKGSPPSQERGVGLFHTAILYPERKDLAEIYKRLLDAS
                     YPLTGASDHGVSEALYLNDPDNNGVELYWDKPKELWPKKQDGSIDMYTLSLDLDDLLN
                     EL"
     gene            complement(7445..8071)
                     /locus_tag="PLX80_11120"
     CDS             complement(7445..8071)
                     /locus_tag="PLX80_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HQY21383.1"
                     /translation="MKTHTKETQDSLTPDLALEILKEGNQRFVNNIKVNRNLLKQVNE
                     TSTNQFPFAAVLSCIDSRTSAELIFDQGLGDIFSIRIAGNILNDDILGSMEFACKKVG
                     SKLIVVLGHTNCGAIIGACNDVKMGHLTSLINKIKPAIECETETTTNRNGSNIEFTEN
                     VSLNNVKLTVEGIKKRSTILAELENENKIKIVGGMYDVETGVVRFLDS"
BASE COUNT         2825 a         1334 c         1478 g         2568 t
ORIGIN      
        1 gtgagtctct ggataaatgg aacatcggag attcagcttt cgattttttg tatcttggga
       61 aaaatgacat tgattataaa ttaccgggca cactgaataa agtatttgat catgaatact
      121 gggttagtgc tgatcagtca gatattataa atgactctta tccgttattg actgtagatg
      181 aatatttatc tgaacctgtt gtgtcagaca aatttaattt tttggaaatt acattggatg
      241 atctcgataa agagctttta gaaaaattat cactggaaaa gaatttcata ttgatcctgt
      301 caactgataa tccaaataca atgtctgagt taagaagagc attcattgaa cttataaata
      361 acaatgtaga gctacctgtc attattaaga gagacttttc aaacaataaa gatctgacag
      421 atgatgaact attggtgcac acttctactg acattggtgg attacttttg gacggattgg
      481 gagacggtgt atggatagaa tcttcaggca cggaaattaa aaagataaac aacataagtt
      541 ttggaatttt acaggcgacc cgaacaagaa ttacgagaac agattatatt tcatgcccgt
      601 cctgcggcag gactttgttt gatcttcagg agacaacagc aaagatcaga aaaagaacgg
      661 atcatctcaa aggcatgaag attgcaatta tgggatgcat tgtaaacgga ccaggagaaa
      721 tggcagatgc tgattacggt tatgtcggca gcggtccggg gaaaattact ttatacaaag
      781 gaagggaagt agtaaagaaa agtatagata ctgaaattgc tgtagatgaa ttaattactc
      841 tgattaaaga aaataatgac tggacggaag tttcaaattg attcgtaaaa ataaataata
      901 gcaaaacatt aaatcttaat aaaatgaacc caataaaatt ttttagaaga ttagcaataa
      961 tattaattat tatttcaagt ggttttctga aagcggatga accttatacg attcaacttg
     1021 agcaggttaa tattccaggc tcaccaaaga tccattcgtt tgcttttgct gatgataacg
     1081 gcaaatggct ttttatcggc ggacgtataa atggaatgca cggctttgat gctgcaacag
     1141 cttttccaaa acaattttca aatacaaata tttttgtagt cgatccttcc acaaacatga
     1201 catactcacg aaatttattc actgatctga atttcacagt tgctgatccg ctgagatcaa
     1261 ctaacatgca gtattattat gacggaacaa agctctacat aacaggcgga tacggatttg
     1321 acagtacatt taatgatctt ctaacatttc cgacattaac agtagtcgat gtcagtcaga
     1381 caattcaggc ggtgatcaat ggcacatctg tagcaccata tatcagacag atcagtgatc
     1441 cgagaatgaa agtctgcggc ggagagatgc aaaagcttgg agattatttt tatcttgcag
     1501 gcggacatat ttttaccggt gcatattcgc caatcagcaa tgatcaggtc tatacaaatg
     1561 aaataagaaa atttaaaata aatgacaacg gtactaatgt cagtatttca gattacagcg
     1621 caagtgaaga tacgatagaa tttcacagaa gagatatgaa tctcgtacct gccataaaac
     1681 ctgacggagt gagtcagtac attacacttt atggtggtgt attcagaagc tatgctgatc
     1741 tgccttttat gaatccaatt tatatagatg aaaacaatgc agtaactgac tttgggtttg
     1801 agcaaaaaat gtctcaatac acttgttctt atttgtctgc ttttaattcg gcaaatggca
     1861 gtatgcatac aacattcctc ggaggtacaa gtctttatta ttttaatgaa gtgttgaatc
     1921 agcagatcag agattcgctt gttcctttta taaatgatat tacaacatta acaagaaatt
     1981 caaatggaac atccattgaa aatatatcac cgactaaaat gccggcactg cttggaacaa
     2041 atgctaaatt tattttaagt ccgtcgattc cgcagtattc aaataaagtc ataaaactaa
     2101 atgaaatctc gggcaacact tttgcaggct atatctttgg tggtatcaga gcatatctgc
     2161 cgaataatac gccgagcttt gcaagtgaat atattttaaa agtttatata aatccggagc
     2221 caataggcat tcagcagatc tccggtaatg tccccgacaa atttagtctc ggacaaaatt
     2281 atccaaaccc tttcaatccg gttactaaaa ttaaatttga aatttctaaa aatgattttg
     2341 tgagacttgc agtatttaat tcaattggaa aagaagtagc tgtgattgtg aatgagaatt
     2401 taagtacagg tatatatgaa actgaattct cagcagaagg attatcgagt ggaatttatt
     2461 attataaatt aacatcagat aatttttcag aaacaaagaa aatgctgctg atcaagtaac
     2521 tgttaaaaga aattgtttga atattaaaaa tatggatttt ataattgatg ctattatcct
     2581 gacttcctgt gaaaaatttt gtcgtgttct taacaaaatc tttttgattt gaaacagact
     2641 ttcaatccta agtgcgttcc caaacatagt ttggaacgag catcagcagc taaaacaatt
     2701 cctgactttt cgtaaagtat tatagacagg gaatcagcga attgtcagat ttaaatatta
     2761 aaaaaaagga aaataattcc gtaaaaatta aaaaatacat tattattcat gagaataaga
     2821 ttacttatca ctttactttt tgtttctcaa ttcacaggtc tgatcaaagc tgatacagaa
     2881 ccttatactg tagatcttga acagattttt attccaaacg caccggcaat tcattcattt
     2941 gcttttgctg agtcaaacgg aagatggctg tttataggtg gtcgtacaaa cggtcttcat
     3001 ggctttaatg ctgtgaattc ttttccaaaa caattttcaa acatctatat ctttgtcgta
     3061 gatcctttga ctaatctgac ctatgcaaga agtatattta ctgatcttcc atttgctttt
     3121 gcagatcagc ttcggtcagc aaatatgcag tcatttcagg atggcaataa actttgcatc
     3181 attggcggat acggatatga cagtacagca aacgggcttg ttacttttcc ttctttaaaa
     3241 atttttgatg ttgaagaaac aattaatgca gtgatgaatg gaaattccgt tgctccttat
     3301 gtcagaggtt ttacagatat tcggcttcag gtctgtggtg gtgaacttat gaaattaggt
     3361 gattattttt atcttgtcgg tggacataaa ttcaccggta catacagatt aaacgttaac
     3421 aatcaggtct atactgatca gattaaaaaa tttaaaatta atgatgacgg aataaatgtc
     3481 agcattacga attacactga atttactgat actgcagaat atcacagaag agatatgaat
     3541 ctgattccgg caataaaacc tgacggagtg acacaatatc tgacattgta cggcggagta
     3601 tttaaaaaaa atgctgacct gccttatctg aatccgatct atattgatga gaattccatt
     3661 acagttgatt acagctttga gcaaaaactg agccagtata ccgcggcaca tttaaatgca
     3721 tttaactcaa tgaccggcag catgcacact actttttttg ccggaacaag tttgtattca
     3781 gtaaatgaca ctaccggtgt tctggaatat gatccgctcg tcccgtttat taatgatatt
     3841 tctactctaa caaaaagttc gaacggcatt tcacaggaaa ttgtaaatgc tgaaaagatg
     3901 cctgcactac ttggtactaa tgctaaattc atactaaata aatctgtccc gcattttgat
     3961 aatgaagtta taaaattaga tcagcttaat tatacaagaa ctttcgttgg atatatctat
     4021 ggcggtataa atgctcagct tccgaataac ggtccgagca ctccgagcga ttatattttc
     4081 aaagtttata ttactccgga ttttccttta cctgttgagc tgaattcatt cacatccgaa
     4141 atttcaggaa gagatgtaaa gcttaagtgg agcacttcgt cagaaattaa taactcggga
     4201 ttttatatag agagaaagac agcctcagtt gtaaacagta gctgggaaaa tatttcattc
     4261 caggaaggaa atggaaattc taattcagta aataattata atttcacaga aaaaaatatg
     4321 aatgccggga gatatacata tagattgaag cagattgatt tcaacggcag ttttgaatat
     4381 tataatttat caggtgatgt tgtaattgct tcaccaaata attattccct gcatcaaaat
     4441 tatccaaatc catttaaccc ttctacaaaa atagattttg aaattcctga atccggaacg
     4501 gtatctctga agatctatga tattttagga aatgaggtga tgacacttat caacgaaaat
     4561 aaacaggctg gattttattc tatcgaattt aaaggcagcg aattttccag tggagtttat
     4621 tattataagc ttgaatcaaa aggatttaca gcaagcagga aaatgctgtt agtgaaataa
     4681 aaaaagtaaa tggtgaataa taatacttta aaaattttat ccatatcaat ttttatattc
     4741 actgttaatt cctgtcagac atttattgac gtaggtgata attttgtaag acaattatct
     4801 tcccctgaga aagtaataaa aaaagtaaag gatcctataa gaaaagatgt taagttgtca
     4861 gcgctatgga taggacatgc ttctttcctt ttacagatat atgacaaagt gattctcatc
     4921 gatcctgtat ttactaataa tgtagcacag gtactccgca gatatgccga acccggtctt
     4981 gatctcaatt cactgaaaaa aatagatatg gttctgatct ctcattctca tatggatcat
     5041 cttagccttg gatctctttc agacatagag gataaatttc acggaacgga tattgtcttt
     5101 cccgcaggag tagaaaatta tttacctgat tatgacatga attttattaa gatgaatttt
     5161 actgattaca ggaaaaaatt tacaggagaa acaaagttaa ttgatagtgt aaagattact
     5221 gctgtgcctg cagttcactg gggaggaaga tacggtctag acggaaaatt tttcagcagt
     5281 aatggatact gcggttttat aattgagtat agagatgtga cagtttatta tacttcagat
     5341 acatcttacg aaccggattt atttaaatac attggagata attacgacat cgatcttgta
     5401 ttagtaaata ttatttattg taaaggatgc actgagatca atcaaggaac gtcacatata
     5461 ttaccgatgg gagcaataaa aattcttgat gacacgaagg cagaatacat gatacctgca
     5521 catttcggaa tgttcacaga tccgaatgta cagatcaggg aattaaaaaa aatgtatctg
     5581 acaaatgatg aatataaaag gaaagtaaag atcctgaata tcggagagca gtttgtaatt
     5641 aaaagttctg aaaaatagaa gccgcaaatg cattttacat acataagaaa ataattttat
     5701 tcaaaaaaca aaaagttaaa acttcattta ataaggatat aaaatttttt atattagacc
     5761 tattcaaaaa ttccaaagta aaaaataatg aaaactaaaa aagattatct aaaggaaaca
     5821 atacagcaca ttgacataaa aaaacacaat gtgattccgc ttgtcgatga tatgcgcaac
     5881 atggctttct catcacgcga actcgctaca gcagcggatt attatgatat gatgcttgct
     5941 gacaaaaact gttcagtcat gctatgtctc gccggatcac ttttctcagc cggtctgaaa
     6001 aatgtcgtgg tggacatggt cagaaataat atggtagatg tgatagtttc tacaggtgct
     6061 attattacag atcaggattt ttttgaagga ctgggattta aacattataa aggatcacag
     6121 tttgtagatg acaatgaaat gcgtgaactt atgatcgaca gaatttacga tacttatatt
     6181 gatgaagagg atctcagagt ttgtgattat acaatctctc agatctgtga tacacttgag
     6241 agaagacctt attcttcgag agaatttatc tggcacatgg gaaaatatct ttcagaaaat
     6301 ggaaagaata atgattcagt agtactggca gcatatgaaa ataatgttcc gatatttgta
     6361 ccggcttttt ctgactgctc ggcaggattt ggatttgttc atcatcagca taaaaatcct
     6421 gattcacatg tttctcttga ctcggcaaaa gattttcttg agctgacaaa aattaaattg
     6481 gctgcgaatg aatcgggtct ctatatgata ggcggaggtg tcccgaaaaa ttttgcacag
     6541 gatacagttg tagcagcaga gatccttgat aaagaagtcc cgatgcataa gtatgcaatt
     6601 caaattacag ttgcagatga cagagacgga gcgctctccg gctctacact taaagaagcc
     6661 agttcatggg gaaaggtaga tatgacctac gaacaaatgg tttactgcga agcaacgatt
     6721 gctatgcctc tgattacagg ttatgcttat cacaaaggca actggaagaa cagggagaag
     6781 agagagtttc agaaaatgtt tgagaatgaa aaagctgagg cataaattta gtattatatt
     6841 ttaaaataaa aaaagcggtc ttcacagccg ctttttttat ttttgtttat tcaatttaaa
     6901 gctcatttaa aagatcatcc agatcaagag ataatgtgta catatctatc gacccatctt
     6961 gttttttcgg ccataattcc tttggtttgt cccagtaaag ttctacacca ttattatcag
     7021 gatcattcag atataatgct tcggagacgc catggtcact tgcacctgtt aacggataag
     7081 atgcatcaag aagtctttta tatatttcag ccagatcttt tctttcagga taaagaatcg
     7141 cagtatgaaa caaaccaaca cctctttcct gagacggtgg acttccttta ctgaaccatg
     7201 tatttagtcc gatgtgatga tgatatccgc cggcagaaat gaatgctgcc tgtaaaccat
     7261 atttgacagt gacttcaaag ccgagtaagc cggaataaaa ttccagcgaa cgttccagat
     7321 cagaaacttt taaatgtacg tgaccaattc ttgtttgctg aggaatttta taatcactct
     7381 tcatttatac ttctttaaat tatttaatca attaacataa ttaataaaaa tcttataata
     7441 aatttcacga atccaaaaac ctgactacac ctgtctcaac atcatacatt cctccgacaa
     7501 tttttatctt attttcgttt tcgagttctg caagtattgt acttcttttc ttaatacctt
     7561 cgactgttaa ttttacatta tttaaagaga cattttcagt aaactcaata ttagagccgt
     7621 ttctgttagt agttgtttct gtttcacatt cgatagccgg tttgatctta tttataaggg
     7681 aagtaagatg tcccattttg acatcattac atgcaccaat tattgcgccg caatttgtat
     7741 gacctaagac tacaataagt ttagatccga cttttttaca ggcaaattcc atacttccta
     7801 atatgtcatc attcaaaata ttaccggcaa ttctaatgct gaaaatatca ccaagtccct
     7861 gatcaaaaat gagctccgca gaagtcctgg aatctataca gctcagcaca gcagcaaaag
     7921 gaaactgatt ggtagaagtt tcattgacct gctttagcag attcctgttt actttaatat
     7981 tatttacaaa tctctgatta ccttctttta aaatttctaa tgcaagatca ggagtaagac
     8041 tatcctgcgt ttcctttgta tgagttttca aataagtgag aaattaaatt ccggaaattg
     8101 taaatgaatt taaaaaataa gagaatatta taaaagttaa aacttttgca gctaaaaaat
     8161 taaaaaaatt caattaaata gttaaaataa atttcttaat gcagt
//
LOCUS       DAORVQ010000151         8149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5049_length_8149_cov_6.186187, whole genome shotgun sequence.
ACCESSION   DAORVQ010000151 DAORVQ010000000
VERSION     DAORVQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_8149_cov_6.186187"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLX80_11125"
     CDS             complement(<1..467)
                     /locus_tag="PLX80_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21384.1"
                     /translation="MAKKQKKAQVKIQEKHPLIRDNPLYNYGMMLIFTVFLIFFTTFK
                     ISGDDDVFWHLATGKHIVETGHVPSEDIFGFVTEGQEWMPFEWGWDVITFGLYNIGGY
                     TAISIFRTIIFLLIFFIFFRIMRKFNVSYTLIFLILTLTAFGIIDRLTPRPHI"
     gene            complement(469..1542)
                     /locus_tag="PLX80_11130"
     CDS             complement(469..1542)
                     /locus_tag="PLX80_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21385.1"
                     /translation="MKYFFISLFLTSVIYLSSCGVETNLSQFPISNEGGPIVSETTYV
                     QQSPVWAQFNKPQAVMVGREPLIYVCDTKNNRIVQLDISGGEIGFISVFNPRAIAQDY
                     NFDLLVIADTTTTIFPDTLSMLYRINLVEAGGVLSNASLIPLMRSDYATPLSSRSRKF
                     TGVGVFSDNTYILTRRGPNNTSSLDPDNALLKVAGENSVTSVSVLGGFQPTGNGIYSI
                     DQMSGITTFTNGPTNFIITRNTPEFGFKVEWFVYEDVKGTYDPRFLPIENVDILNYQL
                     GTPESVALDPSNNIYVVDNTKDSLYKFTNLGEYKKESFGGEGNGVNQLNGPMGVSFYN
                     KVLYIADTENNRIVRYKLSTDLF"
     gene            complement(1565..2563)
                     /locus_tag="PLX80_11135"
     CDS             complement(1565..2563)
                     /locus_tag="PLX80_11135"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQY21386.1"
                     /translation="MNTKLILIFLFLFSVISEMTYAQLFPNLGGQRTGTTSLQFLKIG
                     TGARATGMGESFVAVSDDISSLHYNPAGLVSFKENGITASYTQWFVDTKLANFGGVYH
                     FGGSNAIGINVTSLQTEDMKVTTEFQPGGTGEYFNFSDLSVGLSYARQMTEQFSFGAT
                     LKYVREDLGQVSIDGVLGDIATFYWTGLGTSRFAIMISNFGGQINPSGDVTQVNGVVL
                     NTYQKFPPPTSFQLGFAIEPIMDGENMLTTSIQLNSPTDNAENISIGAEYSFKKFLYL
                     RGGYKINVDAESFSAGVGFKSNISFAHTELDYSIASYDQLGITQRFSLNILFPNFK"
     gene            complement(2697..5507)
                     /locus_tag="PLX80_11140"
     CDS             complement(2697..5507)
                     /locus_tag="PLX80_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY21387.1"
                     /translation="MKKTLLIFLFILFAGDGFSQELYGTIRGTVTDSLKIPLDGVVIK
                     LKGTYLGSVTDIEGKYTILDVSPGNYTMQVSAEGFRTVEYTDLQVESAPEFVYDIILK
                     SSSYSVDQEIEVVGDRPLMDIEQTSSSHIITSDDISKAIVNDVTDIITQQAGVVKDDN
                     EIHIRGGRSYENSYIIDGVSVQDPLSGTGYGLQLSANSLEEVEVITGGYNAEYGQATS
                     GVVNVRTKEGRYDEVNFYLSYKRDNFGVEKNSGWSFNTDILEMNVSGPEPITKYLFNA
                     LGIKTLGEITLFGNLYMGLSDGFYVNEGGDVANQLNSSIFGGTKFAPRQDNNWYWNAK
                     ATWRLTETMKLNYSFNQSVAINQNSSSLQTNLEYIEPTPGYQYEYQEILDNAGTYTQN
                     SMFNTISWTHTTSPKSFYEIKLNNFFTQLRQDANGVNWTDYTEPFDVTKPPFQYYYID
                     SLRTGLIPGNGFYDVGSPYTWHDHFVNEYSAKFDYVYNFTPKSKLKAGVEASFQEMQL
                     VDIYQPWIKPFGLNNDAYQVYPSFGDIYGQHSITFKGMILNYGLRLDYWLPGKYVDDA
                     VNDTSVTNITPQIRAAYYDDSYTIFGRQVKFRLAPRVGISHPITNNQTLFFSYGHFSK
                     RPKPQFVYAKINPQSSQSTFQKYGNPNLNPETTVAYELGIRNQFTNDDVFTVTAYYKN
                     IYDYVATKSIRINSGRLLGQSFITYFNQDYARSRGIEIEYKKRVGSWFNGRFNFAYSV
                     ATGKSASSDQGFVVATTGGAETISETYLPWDRPVQASANLYFNVQKGQGLFGVGRNIL
                     DDIIFKARMFFQSGKRYTEQIYIGELESGRPEYEADVDNIYGKVGANWFWVDLDIEKY
                     FPIYSMEMVVGISIKNVFNTQNSTIINPVTGEAYEYGQPTPNFYNDPLYPDLQAPLIP
                     YPYNPSRYLTPRNILFTVSLKM"
     gene            complement(5833..7191)
                     /locus_tag="PLX80_11145"
     CDS             complement(5833..7191)
                     /locus_tag="PLX80_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21388.1"
                     /translation="MKRYFSYFLIFITCVLIYSCSETITGTLENQPPDTYLSLRPDSI
                     IAPGTTIKTINWWGDDPDGYISGYRISFDSVNWGFTPRNDSTFILSITGNDSTFRFFV
                     AAVDNKGFIDPTPATNLYPVVNSPPSVNFDAGTEIPDTTFPLATFKWTGSDPDGELNL
                     AYYYWSLNDTTNFKRVPATTNLITLTKDSGLALNSDNILYLKAQDDAGAFSPIVQMPD
                     TGQTWHVREQTGRILLVKDMPLSEFAAAESYFNIVMDTLTYDVLDIKSNDGALVPKII
                     NPMFIETLKLYDIVIWSANRGNVATDNASFDLAQNSLPFYLQSGRKLFFTTGLPNAEV
                     QGQGDLINFAPIDSISNCTIALVPGNVSLINIDQGYPVITSNTLLQRIRGLKVTEPSK
                     VIYRLPISTNCEVNTIVAVKDAQNSPKNIFMSMPVYKLNNNINASIELFKKIIISEFG
                     YQ"
     gene            complement(7508..>8149)
                     /locus_tag="PLX80_11150"
     CDS             complement(7508..>8149)
                     /locus_tag="PLX80_11150"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21389.1"
                     /translation="RLNAPVTINCVNAATGVACATVDPLPDTTFRRNFGEILVSDGSN
                     VNARIELQDGNHTFTNNWDGLHNPPQSGYTLLTKNDIIDYVEGVLYYSFSNYKLTPRL
                     NPDFGAVTTVGIVSNVENVESYYLSQNYPNPFNPSTKITFNIPVSGNISFKIYDIIGR
                     EVKSLVNEFRNAGTYNVEFNGADLSSGVYFYRLDAIGRDGNNFVMTKRMVLLK"
BASE COUNT         2486 a         1639 c         1430 g         2594 t
ORIGIN      
        1 attatatgcg gacggggagt gagtctgtct atgatcccga atgcagtcag agtaagtatg
       61 agaaagatga gtgtatagct gacattgaat ttccgcatta tccggaaaaa aataaaaaat
      121 atcagtagga aaattattgt tctgaaaatt gatatcgcag tatatccgcc gatattatac
      181 agaccgaatg tgattacgtc ccatccccat tcgaaaggca tccattcctg accttcggtt
      241 acaaatccga atatatcttc agatggaacg tgaccggtct ctacaatatg tttaccggta
      301 gcaagatgcc agaatacatc atcatctccg gatattttga atgttgtgaa aaaaattaaa
      361 aatacagtaa agatcagcat cattccgtaa ttgtacagag gattgtctct gatcaacggg
      421 tgtttttcct gaatttttac ctgagccttt ttttgctttt ttgccaaatt aaaacagatc
      481 ggttgaaagt ttgtatctta ctattctgtt gttttctgta tctgctatat aaagtacttt
      541 attataaaat gaaactccca tcggaccgtt gagctgattt acaccgtttc cttcaccgcc
      601 gaaagattcc tttttatatt cacccaggtt tgtaaatttg tataatgaat cctttgtatt
      661 atcgaccaca taaatattat tagatggatc aagagcaact gattcaggtg tgccgagctg
      721 ataattaaga atatccacat tttctattgg aaggaatctt ggatcatatg ttcctttaac
      781 atcttcatac acgaaccatt ctactttaaa tccaaactcg ggagtgtttc tggttatgat
      841 aaaatttgta ggtccgtttg taaatgttgt gatgcctgac atctgatcta ttgaataaat
      901 cccattacct gtaggctgaa aaccaccgag tacacttact gatgtaaccg aattttcccc
      961 agcgacttta agcagagcat tatcaggatc gagtgaactt gtattattcg ggcctcttct
     1021 ggtaagaatg tatgtgttat cagaaaagac accaacaccg gtaaacttcc tgcttctgct
     1081 cgaaagagga gttgcataat cggaacgcat aagaggaata agtgaagcat tggataaaac
     1141 gcctcctgct tccacaagat ttattctgta caacatgcta agtgtatcag ggaaaatagt
     1201 agtagtagta tctgcaatga ccagcaaatc aaaattgtaa tcctgcgcaa tagctctcgg
     1261 attaaaaaca gagatgaaac caatttcccc tcctgaaata tcaagctgaa ctatcctgtt
     1321 attttttgta tcgcatacat agataagagg ttctcttcca accattactg cctgaggttt
     1381 attgaactga gcccagacag gagactgctg tacataagtc gtctctgaaa cgatcggtcc
     1441 gccttcattt gaaataggga attgggaaag atttgtttcg acaccacatg aagacagata
     1501 aattacagat gttaaaaata aactgataaa gaaatatttc atacttttat aatataaatt
     1561 aaaatcattt aaaattcggg aataatatgt tcagagaaaa tctttgtgta atgccaagct
     1621 gatcataact tgcaattgaa taatccagct cggtatgagc aaacgaaata tttgatttga
     1681 atcccacacc tgctgaaaaa ctttcagcat ctacatttat tttatagccg cctctgagat
     1741 aaagaaattt cttaaaagaa tactcagcgc ctatactaat attctcagca ttgtcagtcg
     1801 gactgttaag ctgtatcgaa gtggtcagca tattttctcc atccatgatg ggctcaattg
     1861 caaatccaag ctgaaatgat gtcggcggag gaaatttctg atatgtattt aatacgactc
     1921 cgtttacctg agtgacgtct ccggaaggat tgatctgacc gccgaagttt gagatcataa
     1981 ttgcaaatct cgatgtaccg agtcctgtcc agtaaaaagt agcaatgtct ccaagtactc
     2041 cgtcaatact gacctgtccg agatcttcac ggacatactt taaagttgca ccaaatgaga
     2101 actgctctgt catctgtctc gcataagata gtccaacgct caggtcagaa aaattaaagt
     2161 actcgcctgt tcctcccggc tgaaattctg tagtgacctt catgtcttca gtctgcaaag
     2221 atgtaacgtt tataccaatt gcattacttc cgccgaagtg atatacaccg ccgaagttag
     2281 caagctttgt atccacgaac cactgtgtat aacttgcagt aattccattc tccttaaatg
     2341 agacaagtcc tgcaggatta taatgaagag agcttatgtc gtccgacact gcgacaaaac
     2401 tttcacccat acctgtagcc cgcgcgcctg taccgatctt taggaactgc agcgaagtcg
     2461 ttcctgtcct ttgcccgccg agattcggaa aaagctgtgc atatgtcatt tccgaaatga
     2521 cgctgaagag aaacagaaat attaaaatta attttgtatt catctatttt attaaaatca
     2581 ttttttttat ctggtaaaat ttcttgcttg aaatttcatc tgccgaagtc acatttccgt
     2641 ttgtagttgg tcacgctaac tgcgctatct atttacaact acggcggcaa atcattttac
     2701 atctttaaag acacagtaaa caatatattc ctcggagtaa gatatctcga aggattatat
     2761 ggatagggga tcagaggtgc ctgaagatcc ggatacaatg gatcattata aaaattcggt
     2821 gtcggctgtc cgtattcata agcttcgcct gttaccggat ttatgatcgt tgaattctga
     2881 gtattgaata cattctttat cgaaattcca accaccatct ccatagaata tatcgggaaa
     2941 tatttctcta tatccagatc cacccaaaac cagtttgcac cgactttacc atatatgttg
     3001 tcaacatctg cttcgtactc cggtcttccg ctttctagtt caccaatata gatctgctcc
     3061 gtatatctct ttcctgactg gaaaaacatc cttgccttaa atattatatc atctaaaata
     3121 ttccttccca ctccaaaaag tccctgacct ttctgaacgt taaaataaag atttgcgctc
     3181 gcctgaaccg gtctgtccca gggtaaataa gtttcggaaa ttgtttctgc accaccggta
     3241 gtagcaacta caaatccctg atctgaagaa gcactttttc ctgttgcaac agaataagca
     3301 aaattaaatc tgccattgaa ccagcttccg actcttttct tatattcgat ctcaatacct
     3361 cttgaccttg cataatcctg attaaagtaa gtaatgaatg actgcccgag aagtctgccg
     3421 ctgtttattc ggatactctt agttgccaca taatcataaa tatttttata ataagcagtt
     3481 acagtaaaca catcatcatt tgtaaactga tttcttatac cgagttcata agcaaccgtc
     3541 gtctcgggat tcagattagg atttccgtat ttttgaaaag tggactgcga agactgagga
     3601 ttgatctttg catatacaaa ctgcggcttt ggtcttttgg aaaaatgtcc gtaagaaaaa
     3661 aacagtgtct gattgtttgt aatgggatgt gaaataccaa ctcgcggcgc aagtctgaac
     3721 ttcacctgtc ttccgaaaat tgtataagag tcatcatagt aagctgctct gatctgcgga
     3781 gtaatgtttg taacagaagt atcatttact gcatcgtcaa catattttcc cggcagccag
     3841 taatcaagtc tcagaccgta attcaggatc atgcctttaa aagtaatgct gtgctgaccg
     3901 tatatatctc caaaagaagg atacacctga tatgcgtcgt tattcaaacc aaaaggtttt
     3961 atccatggct gatagatatc gactaactgc atctcctgaa aactcgcttc gacaccggct
     4021 ttgagtttac tcttcggagt aaaattatat acgtaatcaa acttcgcaga atactcattc
     4081 acaaaatggt catgccatgt ataaggactt ccgacatcat aaaatccatt tccgggtatg
     4141 agtcctgtcc tcagactgtc aatataataa tactgaaacg gcggctttgt aacatcaaac
     4201 ggttcagtgt aatcagtcca gtttactccg ttagcatcct gtctgagctg tgtaaaaaaa
     4261 ttatttaatt ttatttcata aaaagatttg ggactggtag tatgtgtcca gcttatagta
     4321 ttaaacatac tgttttgtgt gtatgtacct gcgttgtcaa gaatttcctg atattcatac
     4381 tgatagccgg gagttggttc gatatattca agattggtct gcagcgaact tgaattctga
     4441 tttatagcaa ctgactgatt gaaagagtaa ttaagcttca tagtttctgt cagtctccat
     4501 gtagctttag cattccagta ccagttattg tcctgacgcg gtgcaaattt tgtaccgcca
     4561 aaaattgaag agttcaactg atttgcaaca tcaccgcctt cattcacata aaatccatca
     4621 gatagaccca tgtataaatt tccaaataaa gtgatctcac ctagtgtttt tatacctaat
     4681 gcattaaata aatatttagt aatcggttcg ggaccgctga cattcatttc aagaatgtct
     4741 gtattaaaac tccatcctga atttttttct acaccaaaat tatctctctt ataagataaa
     4801 taaaaattaa cttcatcata ccggccctct tttgttctta catttacaac accactggtt
     4861 gcctgaccat attcagcatt ataacctccg gtaataactt ccacttcctc tagagaatta
     4921 gcagataact gaagtccgta gcctgtacct gaaaggggat cctgaactga tactccatct
     4981 atgatatatg aattctcata acttcgtccg ccccgtatat gaatctcgtt atcatctttg
     5041 acaacacctg cctgctgtgt aataatatca gtaacatcat ttacaatagc cttagaaata
     5101 tcatcacttg taattatgtg actgctgctc gtttgctcaa tgtccataag cggtctgtct
     5161 cctacgactt caatctcctg atctactgaa tatgaactgg actttaaaat aatatcataa
     5221 acaaattcag gtgcagactc aacctgcaga tctgtatatt cgacagttct gaatccttct
     5281 gctgacacct gcatggtgta atttccgggt gatacatcaa gtatggtata tttcccttca
     5341 atgtcagtta cagatccaag gtaagttccc ttaagcttga tcacaacacc gtcaagcgga
     5401 atcttcagtg aatcggttac agttccgcgg atggtgccgt aaagctcttg cgaaaaaccg
     5461 tctcctgcaa acagtataaa taaaaaaatg agtagtgttt ttttcataaa cttatgtcaa
     5521 ttgtaatttt taaatctaaa taaatccgat aatggatttt taaaactatg aatatataat
     5581 attaaaatat ttcaaatttt ttatttctat ttattcgccg atgttggtgt tccaaaaagt
     5641 ttgctctttt attttataat ccactgtaca tctaaaacaa actttttggt ctccaaataa
     5701 cttgcatctg ccgaagagac ttttccgttt gtagccggtc acgtacgcct tgctcattaa
     5761 agaccaatac ggcggaaatt cctaattctt aatttctaat ttttaactca tgaagcagcc
     5821 tataggctgc ttctactggt atccaaattc actaatgatt atttttttga ataattctat
     5881 tgatgcattt atattgttgt tcaatttata aacaggcatt gacatgaaaa tattcttagg
     5941 tgaattctga gcatccttca cagcgacaat tgtatttact tcacagtttg tactaatagg
     6001 aagtctgtat attacctttg acggctctgt cacttttaaa cctctgatcc tctgaagtaa
     6061 tgtatttgat gtaataaccg gatagccctg atcaatatta ataagagata catttcccgg
     6121 tacaagtgca attgtacaat ttgaaatact gtctatcgga gcaaagttta tcagatctcc
     6181 ctgtccctgc acttcagcat ttggcagacc ggttgtaaaa aataattttc tgccggactg
     6241 aagataaaaa ggaagtgagt tttgagccag atcgaaactt gcattatctg tagcgacatt
     6301 ccctctgttt gcactccaga tcacaatgtc atataatttc agtgtctcaa tgaacattgg
     6361 attaatgatc tttggtacaa gtgctccatc gtttgatttt atatcaagta catcatatgt
     6421 aagtgtatcc attacaatgt tgaaataact ttcagcagca gcaaattcac ttaacggcat
     6481 gtctttgact aatagtattc tgcctgtctg ttccctcaca tgccatgtct gtccggtatc
     6541 aggcatctgt actatcggac tgaaagctcc cgcgtcatcc tgagctttga gatataaaat
     6601 attatctgag tttagtgcaa ggccgctgtc ttttgttaat gtaataagat tagtagtagc
     6661 aggtactctt ttgaaatttg ttgtgtcatt taatgaccag taataataag caagatttaa
     6721 ttcaccgtcg ggatctgaac ctgtccattt aaaagtagca agagggaagg tagtatcagg
     6781 aatttctgta cctgcatcaa aattaactga gggcggagaa ttgaccacag gataaagatt
     6841 agtcgcagga gtaggatcaa taaaaccttt attatctact gccgctacaa aaaatctgaa
     6901 cgtactgtca tttcctgtaa tggataaaat gaaagtgctg tcatttcttg gagtaaagcc
     6961 ccagttcacg gagtcaaaag aaattcgata acccgagata taaccgtccg gatcatcgcc
     7021 ccaccagttg atggttttta tcgttgtacc cggcgcaata atactgtcag gacgaagaga
     7081 caaataagta tcaggcggct gattttccaa tgtacctgta atggtctcac tacaggaata
     7141 aattaaaaca caggttatga aaattaaaaa gtaagagaaa tatctcttca taaaaattaa
     7201 gattaattaa aacttttata ttgtaaatct aaaacttgaa tgttctatat ctattcgcag
     7261 agtcaagtgt atcttgaact ctgagctttc tgctctttcc aaaactcagt ttgggaatgt
     7321 actttgaatt gaaactccat ttgatatcaa tctctgttat gcaatgactg tcgaattcaa
     7381 gtttccgttt gttgctgtta atgctcgctc cgctctgtta agaccagcaa ccgttcaaat
     7441 tgataactgc gaataaaaat atttccgcag agccactcaa tttcctgact ctgcggaaag
     7501 ctaaatttta tttaagtaaa accattctct ttgtcattac aaaattattt ccatctcttc
     7561 cgattgcatc aagcctataa aaatacacac cgctcgagag atcagctccg ttaaattcta
     7621 cattatatgt tcctgcattt ctaaattcat ttactaagga ctttacttca cgtccgatta
     7681 tatcataaat cttgaaagag atatttcctg atacaggaat gttaaatgtt atcttagtcg
     7741 aagggttgaa cgggtttgga taattttgtg acagataata actttcaaca ttctcgacat
     7801 tgcttacaat tccaactgtt gtgactgctc caaaatcagg attcagtctt ggagtaagtt
     7861 tgtagttgct gaaagaataa tacaatacac cttctacata atcaataata tcattctttg
     7921 taagaagagt atatccagac tgaggcggat tatgaagtcc gtcccagtta tttgtgaatg
     7981 tatgattacc atcctgaagt tcgatccttg cgtttacatt ggaaccatcc gatacgagta
     8041 tttccccgaa gttacgtctg aatgtagtat ccggaagagg atcaaccgta gcgcaggcga
     8101 cacctgtagc tgcgtttaca caattgatcg ttacaggcgc attcagtct
//
LOCUS       DAORVQ010000152         8125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5071_length_8125_cov_6.115118, whole genome shotgun sequence.
ACCESSION   DAORVQ010000152 DAORVQ010000000
VERSION     DAORVQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5071_length_8125_cov_6.115118"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="ftcD"
                     /locus_tag="PLX80_11155"
     CDS             complement(<1..1053)
                     /gene="ftcD"
                     /locus_tag="PLX80_11155"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HQY21390.1"
                     /translation="MKKIVECVPNISEGRDENIINACADAVKKVSGVTLLDVDPGKST
                     NRTVFTFVGDPESVVEAAFQFSKTAYELIDMSKHSGEHPRMGAVDVVPFVPVANVSTE
                     ECVECAKQYGKRVGEELGVPVYLYEEASANPERKMLKQIRSGEYEGIKNKIYKPEWKP
                     DFGPQEFIPASGATVTGSRFFLIAYNVNILGTKEQAHKIALNIREQGRKEDGPGRLPF
                     TKGIGWYVDEYNMAQVSLNLDNYLISPPHVAFEECVNDARDMNLAVCGSELVGLIPLE
                     AMLMAADYYLKKENLFILDERQKIRLVIDRLGLSSISEFDPDKRIIEYMIEEKANEPL
                     ASLTVRDFVKLVGARTS"
     gene            complement(1096..1740)
                     /locus_tag="PLX80_11160"
     CDS             complement(1096..1740)
                     /locus_tag="PLX80_11160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21391.1"
                     /translation="MQKYKKSAKSVFELGCGTGNNLLYLSQFYNAEGVDISNSMLKLA
                     AAKVEKSALHKGDIRNFIPGKKQDVVFCLYDTINHLLLFNEWKKVFKNVFNSLNENGL
                     FIFDFNTFYKLDLISEISPVVNRFNKNFLIADIEKISVNTFNWNLKVFEFKKDDDFKL
                     HESNIKESSFELEKITDALEKHFEILETVDEFDKKIHQHSERIYIVCRKKNLKK"
     gene            complement(1858..2718)
                     /locus_tag="PLX80_11165"
     CDS             complement(1858..2718)
                     /locus_tag="PLX80_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HQY21392.1"
                     /translation="MNLSFNDLKKRFSNIKLIVSDIDGTIISSKNEVGELTKQIVKKL
                     KKKDILLSFASQRVHSSIVPIAEELDVNIPIISLNGALIQDKDSKIILNRSVIDRKYV
                     EKALRLAEKFYLKIALCYNDRIVYTEDNSVVKDFMTRFGTTYSLVDSYDDYLDNVLEI
                     IMTGNDRGNMKEIQRRMRLPFGMYLKVKYFRSQSFHGVFNVEITRKGISKKTGLKILS
                     KYLKIKNDEILVFGDWYNDKELFEFGGTNIALDNAVDELKEMADYITDKSNEDDGVGH
                     FLQKFYDNIN"
     gene            complement(2918..4249)
                     /gene="preA"
                     /locus_tag="PLX80_11170"
     CDS             complement(2918..4249)
                     /gene="preA"
                     /locus_tag="PLX80_11170"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HQY21393.1"
                     /translation="MPDLKINFAGIKSPNPFWLASAPPTNSGYQIMKAFDAGWGGAVW
                     KTLGVPVVNVSSRYGTVNYRDNRMVGFNNIELITDRPLKDNLREIEIVKKHFPDHAVI
                     ASLMVESRAEWHQIVRDVENAGVDGLELNFGCPHGMCERGMGSAVGQEPDILKLIVSW
                     VKEVAKTPVIVKLTPNISDITEPGIAAREGGADAISLINTIQSIVGVDIDNFVPYPVV
                     DGKSTNGGYCGPAVKPIALNMVKSCAQESGIGIPVSGIGGIENWRDAVEFILLGASSL
                     QVCTAVMHYGFGIIREITSGFESYMKDKGFNTVDEMVGKALPNIKTWENLNLNYQIIA
                     EINKEKCIGCQLCYIACEDGAHQAIGLPDLNNNGTRVPYIIEENCVGCNLCSIVCPVE
                     GCISMIRTDDGKISESWKERTDAGNIPVKFDDPLAGGLHHKVPDPLSALKK"
     gene            complement(4397..5722)
                     /locus_tag="PLX80_11175"
     CDS             complement(4397..5722)
                     /locus_tag="PLX80_11175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY21394.1"
                     /translation="MSEYKQPNNESEFENNFSQIKPLMNDTQAYYESSRCLFCYDAPC
                     VSACPTGIDIPLFIRQINSGNVTGSAKTIYDSNYLGNACGKVCPTEVLCEGACVYNLQ
                     DVKAIEIGRLQNYATDKAIKNNIEFFTAGKSNGKKVAVIGAGPAGISCACELRRSGFE
                     VDIFEAKEKPSGLTLYGVAPYKITNEEALNEINYLQKQLGFRIIFSTFISSKEDFKKL
                     EDEYDAVFIGIGLGKTNYVNIKGEDLENCFGAVEFISELKINQHKVKAGSRVIVLGGG
                     NTAMDAASESARLGADEVILAYRRGKEEMGAYDFEYELAKDAGVQGLFNVSPIEITGN
                     EKVSGVKFIKTMTYNGKVENIDGSEFTIDCDMVIKATGQMKQSDLFENISGLEADEKG
                     RIKINKTYQTTNPKYFSGGDVISGGKEVVNAVAEGKAAGKGIEEYLLKI"
     gene            complement(5759..6625)
                     /locus_tag="PLX80_11180"
     CDS             complement(5759..6625)
                     /locus_tag="PLX80_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQY21395.1"
                     /translation="MSNKIKSGLIQMSLPITEGEGTIEEIKEAMVQKHIPLIEDAGRQ
                     GVQILCLQEIFNTPYFCPGQNKEWYESAESVPGPTVERMQEYAKKYNMVIIVPVYEKE
                     QAGVLYNTAAVIDADGQYLGKYRKNHIPHTSGFWEKFFFKPGNLGYPVFQTKYCKVGV
                     YICYDRHFPEGARILGLNGAEIVYNPSATVAGLSQYLWKLEQPAHAAANGYFMGCINR
                     VGTEKPWNLGKFYGTSYFVDPRGQIFAQASEDNDELLVAEFDLAMIEEVRSTWHFFRD
                     RRPETYEKITEL"
     gene            complement(7024..7713)
                     /locus_tag="PLX80_11185"
     CDS             complement(7024..7713)
                     /locus_tag="PLX80_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQY21396.1"
                     /translation="MPNNKRFSFVERTRFFIIENYKTVYTEIPKVACTSFKVWMTDLL
                     KIDFDINKWENIHDHNFPMKTGYDMLREYPDYFKFAFVRNPWDRILSCWLSKIKTPDY
                     DDGKNWKKGVENNLWRYGDMFWSGMSFNDFIYSVIKIPNPDSDAHFRSQYTFFYDKDN
                     KPVLDFTGRFENFNNDFIYVCNKIGSSVTDPPHVHKQNRTHYRDYYDSRTKKLVEERY
                     PYDIKLFNYKF"
BASE COUNT         2496 a         1593 c         1264 g         2772 t
ORIGIN      
        1 tgaagtcctt gcaccgacaa gtttgacaaa atccctgaca gtgagagaag caagaggttc
       61 gttagctttt tcctcaatca tatattcaat gatcctttta tccggatcga attcagaaat
      121 tgaagaaaga ccaagtctgt caatgaccag tctgatcttt tgtctttcat caagtataaa
      181 taaattttcc tttttgagat aatagtcggc tgccatcagc attgcttcga gaggaatgag
      241 tccgaccagt tcactgccgc agactgcgag attcatatca cgtgcatcat ttacacattc
      301 ttcaaaagca acatgaggcg gagagatcag ataattatca agattcagcg agacctgtgc
      361 catgttatat tcatccacat accatccgat tcccttcgtg aaaggcagtc tgccgggtcc
      421 atcttctttc cttccctgct cacggatatt cagagcaatt ttatgcgcct gctctttagt
      481 gccgagtata tttacattat atgcgataag aaaaaatctc gaaccggtaa ctgtggcacc
      541 ggatgctggg ataaattcct gcggaccaaa gtcaggtttc cattcgggtt tgtaaatttt
      601 atttttaatg ccttcgtatt cacctgagcg aatttgttta agcattttac gttcgggatt
      661 tgcagaagct tcttcatata aataaaccgg aacaccaagt tcttctccga ctcttttacc
      721 gtactgcttt gcacattcga cacactcttc tgtagaaaca tttgcaactg gtacaaaagg
      781 aacgacatct acagcaccca ttctcggatg ttcacctgaa tgtttactca tgtcaattaa
      841 ttcataagcc gtcttggaaa attgaaatgc tgcttcgaca acagactcag gatcacctac
      901 aaaagtgaac acagttctgt tggtggactt acccggatcg acatcaagca aagtaactcc
      961 gctgactttt ttgactgcat cagcacaggc attgattata ttttcatctc ttccttcgga
     1021 aatatttgga acgcactcta caattttttt cattactgct tattaatatt attaaaatta
     1081 ttacaaaatc taaaattact ttttcaaatt tttctttcga cagacaatat aaattctttc
     1141 tgaatgctga tggatcttct tatcaaattc atcaactgtt tccaaaattt caaaatgctt
     1201 ttccagagca tctgtaattt tttctaattc aaatgaagac tcttttatat tagattcatg
     1261 aagtttgaaa tcatcatcct ttttaaattc aaatacttta agattccagt tgaatgtatt
     1321 tactgaaatt ttttcaatgt cggctataag aaaattttta ttaaatctgt ttacaacagg
     1381 agatatttca gagatcagat ctaatttata aaaagtatta aaatcaaaaa tgaacagacc
     1441 attttcattc agagagttaa agacattttt aaaaaccttt ttccattcat taaaaagaag
     1501 cagatgatta atagtatcat agagacaaaa tacaacgtct tgcttttttc cggggatgaa
     1561 atttctgata tctcctttgt gaagtgctga cttttctacc tttgcagcag ctaacttcag
     1621 catgctgttg cttatatcca cgccttcagc attgtaaaat tgagaaagat acaacagatt
     1681 atttcctgta ccgcacccta attcaaaaac tgactttgca gattttttat acttctgaat
     1741 actctttcta acaaacttaa tattatcatc agaatgtccg agaacttcat catagaattt
     1801 tgatatattc tggtaaccgt tttctattgg aatttgcttc ttcagatctg taaaatatta
     1861 atttatatta tcataaaatt tctgtaaaaa atgtccgact ccgtcatctt catttgactt
     1921 atccgtaata tagtcagcca tttctttaag ttcatcgaca gcattatcga gagcaatgtt
     1981 cgtcccgccg aattcgaaca attctttatc attataccag tcaccgaata cgagtatctc
     2041 atcattttta atcttaagat attttgaaag aattttcaga ccggtctttt tgctaatacc
     2101 tttacgtgtt atttcaacat tgaaaacacc atggaatgac tgtgatctga aatatttcac
     2161 tttgagatac attccgaaag gaagtctcat tcgtctttgg atttctttca tattacctct
     2221 gtcatttcct gtcataataa tttcaagtac attatcgaga taatcgtcat aggaatcgac
     2281 aagtgagtag gtagttccga atcttgtcat aaaatctttt acaactgaat tatcttcggt
     2341 ataaacaatt ctgtcattat agcataatgc tattttgaga taaaattttt cggcaagacg
     2401 aagagctttt tcgacatact ttctgtcgat gacagagcgg tttaaaatta tcttgctgtc
     2461 tttatcctgt atcagtgcac cattaagaga aattatgggg atgtttacat cgagttcttc
     2521 ggcgattgga acaatggagg aatgaactct ctgtgaggca aaggacagga gaatatcttt
     2581 cttttttaat ttcttaacaa tttgtttagt aagttcaccg acttcatttt tactggaaat
     2641 tatagtaccg tcaatatctg atacaataag ttttatattg ctgaaccttt tttttaaatc
     2701 attaaacgat aaattcaaat cctgataatt aaataattca tatattttta aatcgaataa
     2761 gttatggctt gggcagtaag agctgtattg ttagagattt attcttttat tcttagggct
     2821 aataatactc agaataacga agattttcaa agtaaaatta aaaacattta ataaatgaga
     2881 agaaaaattt tccgttaatt tttttaaaaa acagtactca ctttttcaaa gcactcaatg
     2941 gatcaggcac tttatgatga agaccgccgg caagagggtc atcaaatttt acagggatat
     3001 ttccagcgtc ggttctttct ttccatgact ctgaaatttt accgtcatca gttctgatca
     3061 tcgagataca tccttcgaca ggacagacta tagaacataa attacagccg acacaatttt
     3121 cttcaattat gtatggaact cttgtaccgt tattatttaa atcaggaagt ccgattgcct
     3181 gatgcgcacc gtcttcacat gcaatatagc aaagctgaca accaatacat ttttctttat
     3241 ttatttcggc gatgatctga taattcagat tcagattttc ccatgtctta atattcggaa
     3301 gtgctttgcc aaccatttca tcaacagtgt taaatccttt gtctttcata tatgattcga
     3361 atcctgaagt gatctcgcgg atgataccga agccgtaatg catgactgca gtacaaacct
     3421 gaagtgaaga ggcaccaagc aaaataaatt caacagcatc tctccagttt tcaataccgc
     3481 cgatccctga aacaggaatt ccgataccgg attcctgagc acagcttttt accatattaa
     3541 gtgcaatcgg tttaacggca gggccgcaat aaccgccgtt tgtacttttt ccatcgacga
     3601 caggataagg aacaaaatta tcaatatcca ctccgacaat gctttgaatt gtattgatca
     3661 gagaaattgc atcagcaccg ccttcgcgtg cagcgatacc cggttcagta atatcggaaa
     3721 tatttggagt aagttttact attacaggag tctttgcaac ttcctttacc catgagacaa
     3781 ttaattttag aatgtcaggt tcctgtccaa cagctgatcc cattcctctt tcgcacatac
     3841 catgaggaca gccgaaattt agttcaagtc cgtccacacc tgcattctcc acatcacgaa
     3901 ctatctgatg ccattcagca cgagactcta ccattagcga cgcaatgact gcatgatcag
     3961 gaaaatgttt tttaacaatt tcgatctcac gaagattgtc tttcagaggt ctgtcggtga
     4021 ttagttctat attattgaaa ccgaccattc ggttgtctct gtagttaaca gttccgtatc
     4081 tgcttgagac attgacaaca ggaacaccga gagttttcca aacagcacct ccccatcctg
     4141 catcgaatgc tttcatgatc tggtaacccg aatttgtagg tggtgcagaa gccagccaga
     4201 acgggttggg cgatttgata cctgcgaaat ttatttttag atccggcata tgtttactgg
     4261 ttttggtttc tgacagcaaa acctgtcagg tacaattcct gttaaattaa ttcaaaaatt
     4321 taaatcccat ctttccttcc ccgccatctc caccaatatc taaacctgac atgttcccat
     4381 tctattccaa tccaaattaa atcttcaaca aatattcctc aatacctttt cccgctgcct
     4441 ttccttctgc aactgcatta acaacttcct tccctccact gatcacatct cctcctgaaa
     4501 aatatttagg attagttgtc tgataagttt tgttaatttt aatccttcct ttttcatctg
     4561 cttcaagtcc gctgatattt tcaaataaat cagactgctt catctgacct gttgctttta
     4621 ttaccatatc acagtcaatt gtaaattctg atccatctat attttcaacc ttcccattat
     4681 atgtcattgt ctttataaat tttactcctg atactttttc atttcctgtt atttctatag
     4741 gagaaacatt aaacaatccc tgcacgcctg catcttttgc aagttcatat tcaaaatcgt
     4801 atgctcccat ttcctcctta cctcttctat atgcaagaat gacttcatca gctccaagtc
     4861 ttgcagattc tgaagcagca tccatggcag tatttccacc gccgagaaca atgaccctgc
     4921 ttccggcttt tactttatgc tgatttattt ttaattccga aatgaattcc actgcaccaa
     4981 aacaattttc aagatcctct cctttaatat tcacataatt tgtcttacca agtccgattc
     5041 cgataaagac tgcatcatat tcatcttcaa gttttttaaa atcttctttt gatgaaataa
     5101 atgtactaaa aattattctg aatccgagtt gtttttgaag gtagttaatt tcatttagag
     5161 cttcttcatt agttatctta taaggagcga caccataaag ggttagccct gaaggctttt
     5221 cttttgcttc aaaaatatca acttcaaatc cggatcttcg gagttcacag gcacatgaaa
     5281 ttcccgccgg acctgctccg atgacagcaa cttttttacc attagattta ccggctgtaa
     5341 aaaattcaat attatttttg atcgctttat cagttgcata attctgaagt cgtcctattt
     5401 caatagcttt tacatcctga aggttatata cgcatgcacc ttcacacaga acttcagtag
     5461 gacagacctt accgcatgca ttgccgagat aattggaatc atagatcgtc tttgccgatc
     5521 ctgttacatt tcctgaattg atctgtctga tgaataaagg aatatcgatg cctgtcggac
     5581 atgcactaac gcacggcgca tcatagcaaa agagacatct cgaactttca taataagcct
     5641 gtgtgtcatt catcagaggt tttatctgag aaaaattatt ctcaaattct gattcattat
     5701 tcggctgttt atattcagac atttttaatt taaaataaat ttagtttgaa ttaaaacttt
     5761 ataactcagt aattttttca taagtctcag gtcttctgtc acggaaaaaa tgccatgtag
     5821 atcttacttc ttctatcatt gcaagatcaa attcagcaac aagtaattca tcattatctt
     5881 cagatgcctg tgcaaatatc tgtccgcgcg gatctacgaa ataagaagta ccatagaact
     5941 taccaagatt ccatggcttt tcagttccga ctctgttaat acatcccatg aaatatccat
     6001 tggcagcggc atgagccggc tgttcaagtt tccataaata ttgtgaaagt cctgcgacag
     6061 tagctgatgg attataaacg atctcagcac cgttcaatcc gagtatcctt gcaccttcgg
     6121 ggaaatgtct gtcatagcag atatagactc ctactttgca gtactttgtc tggaaaacgg
     6181 gataaccgag attaccgggt ttaaagaaaa atttttccca gaagcctgat gtatgcggaa
     6241 tatgattttt tctgtatttg ccgagatatt gtccgtctgc gtctattacc gcagcagtat
     6301 tatacaaaac acctgcctgt tctttttcat agacaggaac tatgatcacc atattatatt
     6361 tttttgcata ctcctgcatt ctttcgactg tcgggccggg gacagattct gcggattcat
     6421 accattcttt attttgtccg ggacaaaaat acggtgtatt gaaaatttcc tgaagacaaa
     6481 ggatttgaac tccctgtctg cccgcatctt cgatgagggg aatatgtttc tgcaccattg
     6541 cttctttgat ctcttcgata gtaccttcgc cttcggtgat cggaagactc atttgtataa
     6601 gaccggattt gattttatta gacataaaaa ttttcaatta aaattctttt caaaaattta
     6661 aagcagggat taaaataatt cttatttatt taataaaaaa gattattaaa agaacaatgt
     6721 tcaatgttca atgttcaatg tttaaatcgt tctaaaaatt tattacataa ttattttata
     6781 aaaaagcctg cagattttgc aggctatcat aattcacatt tattaaacct tcattcccca
     6841 gtgcatcata aactttaaga gaaacaaatc ctgatttcga attccacatt ccagatttgt
     6901 cacagggttg aatggatttg gataattttg tgaaagcgaa tatgaagaag gtatatcaac
     6961 ttctgaatgt gtaatgccaa cctgatcgac atgaggaata aatccgtatt gataacttat
     7021 agtttaaaat ttataattga ataatttgat atcatatgga tatctttcct ctaccagttt
     7081 ctttgttcgg ctgtcgtagt aatctctgta atgagttctg ttttgcttat gtacatgagg
     7141 tggatcagtt acagatgaac cgattttatt gcatacatat ataaaatcgt tgttaaaatt
     7201 ttcaaacctg ccggtaaaat ccagtactgg cttattatct ttatcgtaaa agaaagtata
     7261 ttgagaacgg aaatgtgcat ctgaatctgg attcggtatt ttaattacag aatatataaa
     7321 atcattgaaa gacattcctg accagaacat atctccgtat ctccataaat tattttcaac
     7381 tccctttttc cagtttttgc cgtcatcata atcaggtgtc tttattttgc ttaaccagca
     7441 ggacaaaatt ctgtcccatg gatttctgac aaaggcaaat ttaaaatagt caggatattc
     7501 cctcagcata tcatatcctg ttttcattgg aaaattatga tcatgaatat tttcccattt
     7561 attaatatca aaatcaatct ttaacagatc agtcatccag actttaaatg aagtacaggc
     7621 aacttttggt atttcagtat atacagtttt ataattttca ataataaaaa accgcgtccg
     7681 ttctacaaat gagaatcgtt tattattagg catagttaaa cttcttttta aaatatttaa
     7741 atgctgttat cttttaatta aaatttccgg gtttaaattt ttgggatggg gtttgtaggt
     7801 aaatgtaaaa tacaaattta aacatttaaa gaaaagtaaa tcctgattgt atgtcaaccc
     7861 aaagaaaacc aggctgaata aagacatttt cagaagtaat taaaaaatca ttaattataa
     7921 taatataata ttcttgatac tgtgataatt ttttaaatag tgagagtatt aaaaaatcag
     7981 actaaaatcc caaatgaatt caaaaaaaaa caaatccatt tacatcacag aagatatgtt
     8041 cagaatatca tttattttgg gacatttgtc actatttcag cattatcatt ttcatagtat
     8101 ttgaaattcc attagtactc agtgt
//
LOCUS       DAORVQ010000153         8120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5077_length_8120_cov_5.141104, whole genome shotgun sequence.
ACCESSION   DAORVQ010000153 DAORVQ010000000
VERSION     DAORVQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_8120_cov_5.141104"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4176
                     /locus_tag="PLX80_11190"
     CDS             <1..4176
                     /locus_tag="PLX80_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQY21397.1"
                     /translation="FTVPNEHNVEIKINGIVVTNFLRNDFNRIDTTITFPVNMLSDST
                     VNTATATYTSSGGFSGTMFFDLFEFKYPQKLKFRNNFAKMKLIGEDTTSRKIKVSGLN
                     PGNQTFIYDVSNNIRITSYSNSADTLIFSARQNSSIEITNKFITKKPLRIIQRQVPDL
                     VSSSNGVDYLIIYPKIFESQVLQLQNHRETYDNFRVKKAEIQDIYDIFSYGIVSPDAI
                     KNFNRHIFYNWQQPRIKYVCLFGRGSLDPKKSSDATIYSENFIPIKGNPSSDNFYSNV
                     NTGTFVFYNQISIGRLPALNVTEAQSMVNNIISYETQPPRSWSKDFTFIGGGVNQVEQ
                     ELFNPQIIDSLINPHILPNPIKGNPVRIMRYDLNGALTFNITDSVKNQINRGTIAVNF
                     LGHAGSQDWEISMKDPNVLNNNGNFPVVFSMTCYTGKTGEPNFRSFGEKFMNMNNRGA
                     VAFFATSGWGFVYAGSWLNANIYRAFAKDTIRRIGDIISYANNVIKADSLGFTIRHTV
                     NCYTLMGDPALKLQLPVQPEFSISSDDYLLSKKNPSINEPVNLKFYPKNFGLNSDSVR
                     VEMSINRNSQKYFYKENVLYDFSSNDTLSYDFKLDSKGQYQVKFIIDSFNYYPDENKN
                     NNTLIFDLNINNISFVPYKPVINSVLKNDSVEYVGLNPFIKKNEKTITLLLQTDTTDK
                     FNSPIKQIFVNNAPQGVVTKFKTLLPVRDTSKLYFWRTNSIVNGDSSGWSAVQMFKYN
                     PLFTSLNINEKDGENILDTSTAIYKINSQQFPSYELNNVSYNTENNSSGLKINDFNGD
                     LVVRSLGSSGSEASHFSVLGNSIHIDAGGNTGLNLLKVRKLDGKILQHKNFKLTFGNS
                     NDSIINFLNTFDTTHYLMGLNAAYVANTFLLNANTIAKFAQFGSTKLNNTNVRIGFFD
                     SWSFIGYLGAQQSDVSENFSKYVSVWTESISSQNKKFKKTSGTVIFNAGPARQWQNFS
                     WNNVLLPSNKILFDVYGLKADGKSDLLLQNISTNNNTNISSINSILYPNLNFVGKFSI
                     DTITGNKSPVLNSFKLNYKSPAEIIAELDGLWKSDSVAVSGKEVKVGFKIYNAGDLYI
                     PGYVAKIYYLKDFNNVLIKTDTLNNPININGNISHESKFKIPNLRYNISNNETFFIEI
                     LPRGNLNEAFTYNNFVYFDMLPGQQNSAAGLDIFSDGREIQSGDNVSLNPEVKIIVKN
                     DPDLSNSDLFTDLKEIYLNGISINSKSTADRNSDRILKSENSLTKKISFSNELKFTPD
                     LNKGENLLKIITGNKDGSYDTTDFVLNVTDELSIGDLINYPNPMKSETSFMFDVTGFE
                     NPGKGLIKIYTIAGRLIREIEIPVNIGFNTITWDGKDSDGDLIANGTYLYKLSVTGSI
                     QLESEVMKLVVLK"
     gene            complement(4198..4653)
                     /locus_tag="PLX80_11195"
     CDS             complement(4198..4653)
                     /locus_tag="PLX80_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21398.1"
                     /translation="MKHFKFSKLFTILIALEIIFSIYGCDKISQGLQRNKFEFLYRMN
                     NDVTLLREYTSGINKAEDLKFFESKLNNLYASVNKMETVKNYGGSDALKEQFLNLIDS
                     DLEAVKILKDKNLPADDIIKDEYEVQIMKDNVNSFIEHLNAEIVKVGKE"
     gene            complement(4640..5674)
                     /gene="tsaD"
                     /locus_tag="PLX80_11200"
     CDS             complement(4640..5674)
                     /gene="tsaD"
                     /locus_tag="PLX80_11200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQY21399.1"
                     /translation="MNILAIETSCDETSAAVLENENVLSNIISSQISHGKFGGVVPEL
                     ASREHLNNIAKIASESLETSGKHIKDIDVIAATSEPGLIGSLLIGMNFAKSLAFTLNK
                     PFIPVNHIQAHLYSNFLNGQKHEFPFIGLIVSGGHTLLILVENFFKHKIIGYTVDDAA
                     GEAFDKTAKLLGLGYPGGQMIDKFAKDGNKDFHKFPPAIVRDSEFNFSYSGIKTSVLY
                     YLRNIDFENKKNDKLVSDISASFQEAATKSLFKNTLKAAKKYGIKIISVSGGVSANSS
                     LKEKFSELKNSGYSIYFPKPEYSSDNAAMIGITAFFKYKYSQITNDTNYFENDSLTHL
                     AKPRLDYEAF"
     gene            5844..7100
                     /locus_tag="PLX80_11205"
     CDS             5844..7100
                     /locus_tag="PLX80_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQY21400.1"
                     /translation="MFLILLISFLFVLPAISQVKLLIPMESNQSDHLKAYGIAYRHLM
                     NTKEVDWLLNYRGGSFMFGYSSSLEEECKAKGVSYELLDGTMTAQVYSDSKNEENNTD
                     VVRLEKVAKIAVYVPPNALPWDDAVQMVLEYAEIPYDKLWDEEVLTDKLKGYDWLHLH
                     HEDFTGQYGKFFASYGSAPWYLNQQKINEDMAAKLGYKKVSQLKLAVVKKLKEYIANG
                     GFLFTMCSATDTYDIALASQFTDICESIYDGDPADPQANSKLDFTECLAFENFTVDLN
                     PLVYEYSNIDVTGQLLQMGQFNDNFTLVDFSAKYDPVPSMLTQCHTSVINGFLGQTTG
                     FQKNLLKKNVTVMALKDGTEYVRYIHGNYGRGTFTFYGGHDPEDYQHAVGDPKTELKE
                     FPHSPGYRLILNNVLFPAAKKKKLKT"
     gene            7357..>8120
                     /locus_tag="PLX80_11210"
     CDS             7357..>8120
                     /locus_tag="PLX80_11210"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQY21401.1"
                     /translation="MLSGKILIDHRKIKFIVFAFALSLHLFSCNKTPEKTEKKFSIKD
                     DAGIDVEFSSYPERIISLGPNITETIYALGADTLLYGVTEFCDYPPEVKTKVNTGSYL
                     SPDYEIISSLKPDLIIVNVESSNNPTYQSLKNMGYNLFLSHAKDIEGIFKMVNDIGMI
                     TNRENNANILFNSISDQLKYKDPSSVQKKDKTVLILIAINPMMTTNKNTFINEIALLA
                     GLKNIYSEEDQEYPVINYEDVVSKNPSFIIFPADTL"
BASE COUNT         2821 a         1300 c         1338 g         2661 t
ORIGIN      
        1 tttacagttc ccaatgaaca taacgttgaa ataaagatca atggaattgt cgtaacaaat
       61 tttttgagaa atgattttaa cagaattgat acaaccataa cattcccggt aaacatgcta
      121 tctgattcta ctgtaaatac agctactgcg acatatactt cttcaggtgg attttcggga
      181 actatgttct ttgatctttt tgaatttaaa tatcctcaaa agttaaaatt cagaaataat
      241 tttgccaaga tgaaactcat tggtgaagat acaacatcaa gaaagatcaa agtatccgga
      301 ttaaatccgg gtaatcaaac ttttatttat gacgtatcaa acaacatcag aattacatct
      361 tattcaaata gtgccgatac tctgattttc tcggcaaggc aaaattccag tattgaaatc
      421 acaaataaat ttattacgaa aaagccttta agaataattc agagacaggt acctgatctt
      481 gtatcatctt caaacggagt tgattatcta ataatttatc cgaaaatatt tgaatctcag
      541 gtacttcagc ttcagaatca ccgtgaaaca tatgataact tcagagtaaa gaaggctgaa
      601 atacaggata tttatgacat ttttagttac ggaattgtaa gtccggatgc aataaaaaat
      661 ttcaacagac atatttttta taactggcag cagccaagaa taaagtatgt ctgtcttttt
      721 ggaagaggct cactcgatcc gaaaaaaagt tcggatgcta ctatatactc tgaaaatttt
      781 atacctataa aaggaaatcc ttcgtctgat aatttttatt cgaatgtgaa tactggtaca
      841 tttgtttttt ataatcagat ttcaattggc agattacctg ctttaaatgt aactgaagct
      901 caaagtatgg taaataatat tatcagttat gaaacgcagc cacctcggtc atggtcaaaa
      961 gattttacat ttatcggagg cggagtaaat caagttgaac aggaattgtt taatcctcag
     1021 ataattgatt ccctcatcaa tcctcatatt cttccaaatc caattaaagg aaatcctgtt
     1081 cgcataatga gatatgatct aaatggagca cttactttta atataactga ttctgtaaaa
     1141 aatcaaatta acagaggaac aattgctgtt aattttcttg gacatgcggg aagtcaggat
     1201 tgggaaatat ctatgaaaga tcctaacgtc ttaaataata atggaaattt tccggttgta
     1261 ttcagcatga catgctatac cggtaaaaca ggtgaaccaa atttcagatc ttttggagaa
     1321 aaatttatga acatgaataa cagaggagca gttgcttttt ttgctacttc aggctgggga
     1381 ttcgtgtatg ccggatcatg gctgaacgca aatatataca gagcatttgc aaaggacaca
     1441 atcagaagaa tcggagatat tatttcttat gccaataatg ttatcaaagc tgattcttta
     1501 ggatttacca tcagacatac tgtcaattgt tacacattaa tgggtgatcc ggctcttaaa
     1561 cttcagctac ccgtgcagcc cgagttttca atttcttctg atgattattt actttcaaaa
     1621 aaaaatccgt caattaatga acctgtaaat ctaaaattct atccgaaaaa ttttggtttg
     1681 aattcagatt ctgtgagagt tgaaatgagc ataaacagaa atagtcaaaa atatttttat
     1741 aaagaaaatg ttttgtatga tttcagcagc aatgatactc tttcttatga ttttaaactt
     1801 gattcaaaag gacagtatca ggtaaagttt attatagata gttttaatta ttatccagat
     1861 gaaaataaaa ataacaacac acttatcttt gatttaaata ttaataatat ttcttttgtc
     1921 ccatataaac ctgttataaa ttctgtatta aaaaatgaca gcgtggaata tgtcggtctt
     1981 aacccattta taaaaaagaa tgaaaaaact ataactcttt tactgcagac tgatactact
     2041 gataaattca attctcccat aaagcaaatc tttgttaata atgcaccgca gggagttgtc
     2101 acaaaattca aaactctact gcctgtaaga gatacttcaa agctatactt ttggcggacg
     2161 aattcaattg tgaacggaga tagttcagga tggtcggcag ttcagatgtt taaatataat
     2221 ccgctgttta catctttaaa tataaatgaa aaggatggtg aaaatattct tgatacaagt
     2281 acagcaattt ataaaataaa ttctcagcaa tttccatcat atgaattgaa taatgtttcc
     2341 tataatactg aaaataattc tagcggacta aaaataaatg attttaatgg agacctggta
     2401 gtcagatcat taggaagtag tggatctgaa gcttcccatt tttctgtact tggtaatagt
     2461 attcatattg atgctggcgg aaataccggt ctgaatcttc ttaaagtaag aaaacttgac
     2521 ggtaaaattc ttcagcataa aaattttaaa ctgacctttg gaaattctaa tgattcaatt
     2581 attaattttc tgaatacttt tgatactaca cattatttaa tgggactcaa tgcagcttat
     2641 gttgcaaata cttttttact taatgcaaat acaattgcaa agtttgctca gtttggaagt
     2701 actaaactca ataacactaa tgtgagaatt ggctttttcg attcatggag ctttatcgga
     2761 tatcttggtg ctcagcagtc agatgtatca gaaaatttca gcaaatatgt ttcagtatgg
     2821 acagaatcaa tttcatcgca aaataaaaaa ttcaaaaaga catccggaac tgtaattttt
     2881 aatgcaggtc ctgcaaggca atggcagaat ttttcctgga ataatgtttt acttccttcc
     2941 aacaaaattt tatttgatgt gtatggtttg aaggcagacg gaaaatcaga tcttctgctt
     3001 cagaatattt ccactaataa taatactaat atatcttcaa ttaattctat cctttatcca
     3061 aatttaaatt ttgttggaaa attcagcatt gatacaatta caggaaataa atctcctgta
     3121 cttaattcat ttaaactaaa ttataaaagt ccggctgaga tcatcgctga gcttgacgga
     3181 ctgtggaaat cagactctgt tgctgtctca ggaaaagaag tcaaagtcgg ttttaaaatt
     3241 tataatgcag gtgatttata tattccaggt tatgtagcca aaatctatta tcttaaagat
     3301 tttaataatg ttctgattaa aacagatacc ttaaataatc ctattaatat aaatggaaat
     3361 atatcacatg aatcaaaatt taaaatccca aacttgagat ataatatttc aaataatgaa
     3421 acttttttta ttgagatcct tccaagggga aatttaaacg aagcctttac atataataat
     3481 tttgtgtatt ttgatatgct gcccggtcag cagaattcag ctgccggttt ggatatattt
     3541 agtgacggca gggaaattca aagcggagat aatgtaagtc ttaatcctga agtaaaaata
     3601 attgtaaaaa atgatcctga tctgagtaac tctgatcttt ttacagatct taaagaaata
     3661 tatctgaacg gaattagtat aaatagtaaa tcaactgcag acagaaattc agatcgtata
     3721 ttaaaaagtg aaaatagttt aaccaaaaaa attagtttca gtaacgaatt aaaatttact
     3781 ccggatctga ataaaggtga aaatcttctc aagattataa ccggtaataa agacggctca
     3841 tatgatacta cagatttcgt gttgaatgtc actgatgaat tatcaattgg tgatctgatc
     3901 aattatccta atccaatgaa atcggaaaca agttttatgt ttgatgtcac aggctttgag
     3961 aatccgggaa aaggcttaat taaaatttat acaattgctg gcagactgat acgagagatc
     4021 gaaattcctg tgaatattgg gtttaatact attacatggg atggcaaaga cagtgacgga
     4081 gatcttatag caaatggaac ttatctgtat aaactttctg taactggttc tattcagctt
     4141 gagtctgaag taatgaagct tgtagtcctg aaataaaaaa aatagggcag atagatatta
     4201 ttcttttccg acttttacta tctcagcatt cagatgttcg atgaaactat tgacattgtc
     4261 tttcataatt tgaacttcat attcatcctt tataatatcg tcagccggca gatttttatc
     4321 ttttagaatt ttaactgctt ctaagtcact gtctatcaga tttaaaaact gctcctttaa
     4381 agcatcggat ccaccatagt tttttacagt ttccatttta tttacagatg cataaagatt
     4441 gtttaacttt gattcaaaaa atttcagatc ctctgcttta ttaattccag atgtatattc
     4501 tctcagtagc gttacatcat tattcatcct gtaaagaaat tcaaatttat ttctctgaag
     4561 tccctgactg attttatcac agccgtatat tgaaaaaatt atttccaatg caattaatat
     4621 tgtaaatagt ttactgaatt taaaatgctt cataatctaa tctgggttta gctaaatggg
     4681 ttaatgaatc gttttcaaaa taatttgtat catttgtaat ttgcgaatat ttatatttga
     4741 aaaatgctgt aatgcctatc attgctgcat tgtctgatga gtattcgggt tttgggaaat
     4801 agatgctgta acctgaattt tttagctcac tgaatttttc ttttaaactg ctgtttgccg
     4861 acactcctcc tgatacagaa attattttaa ttccatattt ctttgcagcc tttaatgtat
     4921 ttttaaaaag acttttagta gcagcttcct gaaatgaagc ggatatgtct gatacaagtt
     4981 tgtcattttt tttattttca aaatcgatat ttctcaaata ataaagtact gaagttttta
     5041 tacccgaata tgaaaaattg aattcactat ctctgactat tgcaggagga aatttatgaa
     5101 aatctttatt tccatctttt gcaaatttgt cgatcatctg tccgcccgga tatcccagtc
     5161 cgagaagttt tgctgttttg tcaaaagctt caccggcagc atcatccact gtgtaaccga
     5221 taattttatg tttgaaaaaa ttctcaacta aaatcaacaa agtatgacca cctgaaacta
     5281 tcagcccaat aaatggaaat tcatgtttct gaccattcag aaaattggaa taaagatgtg
     5341 cctgaatatg attgacagga ataaaaggtt tattcaatgt gaaggccagt gattttgcaa
     5401 aattcattcc aatcaataaa gaaccaatca atccgggttc tgaagtagca gctataacgt
     5461 ctatatcttt aatatgctta ccggatgttt ctaatgattc acttgcaatt tttgcaatgt
     5521 tgtttaaatg ttcacgggat gccagctcag gaacaacacc accaaacttt ccatgcgaga
     5581 tctgagatga aattatattg gaaagtacat tctcgttttc aagtactgct gcagatgttt
     5641 cgtcacatga agtttcgatt gcgagtatat tcataaaaaa attaaatatt aataattatt
     5701 tttaaacatt aataggaaag ttatgttaca aaaaaaaaat aattacgttt ctattaattt
     5761 tatgaattaa catattttaa aaaataaatt tgacctatta gtttatgacc caaaaagtaa
     5821 aaatggataa taacagattc aaaatgtttt taatactact aatttcattc cttttcgtac
     5881 ttccggctat ttcacaggta aaattactta tcccaatgga atccaatcaa tcagatcatc
     5941 tgaaagctta cggtattgca tacaggcatt tgatgaatac aaaagaagtt gactggcttt
     6001 tgaattacag aggcggatca ttcatgtttg gatattcatc ttcactggaa gaagaatgca
     6061 aagcgaaagg agtatcttac gaactgctcg atggaacaat gactgctcag gtatattcag
     6121 attcaaaaaa tgaagaaaat aatacagatg tagtaagact ggaaaaggtt gctaagatcg
     6181 ctgtgtatgt cccgcccaat gcactccctt gggatgatgc tgttcagatg gtactggaat
     6241 atgcggagat tccttatgat aagctctggg atgaggaagt gcttactgac aaactaaaag
     6301 gttatgactg gcttcatctt catcatgaag attttaccgg acagtatgga aaattctttg
     6361 catcatacgg ctcagctccg tggtatctga atcagcaaaa gatcaacgaa gatatggcag
     6421 ctaaactcgg atataaaaaa gtctcacaat taaaacttgc agttgtaaaa aagctcaaag
     6481 agtatattgc caacggagga tttttattta caatgtgctc tgcgactgat acttatgaca
     6541 ttgcacttgc ttctcagttc acggatattt gtgaatcaat ttatgacgga gaccctgccg
     6601 atccgcaggc aaacagtaaa ctcgatttta ctgaatgtct ggcatttgaa aattttactg
     6661 tcgatctgaa tccgttggtt tatgaatatt cgaatattga tgtgaccggt cagcttctgc
     6721 agatgggtca gtttaatgac aatttcactt tggttgattt ttcagcaaag tatgatccgg
     6781 ttccttcaat gcttacacaa tgccacactt cagtaataaa cggattcctt ggacagacta
     6841 caggttttca gaaaaacctg ctgaaaaaaa atgtaactgt aatggctttg aaagacggaa
     6901 cagaatatgt gagatatatt cacggaaatt acggcagggg aacattcaca ttttatggcg
     6961 gtcacgatcc tgaagattat cagcatgctg ttggtgatcc aaagaccgaa ctaaaagaat
     7021 ttccacattc tcccggctac agattgatcc tgaataatgt attatttcca gctgcaaaaa
     7081 agaaaaaact taaaacgtaa aagcagttag tagttaatag ttaacagtta ttattggtgc
     7141 taaaattcta ataaatatac ctcaacttct caatgttaaa ccctcccctt accaagggga
     7201 gggcagggtg gggttgaaaa aacaagtaaa gcaataaatc tttgttcaaa aaatattttt
     7261 cataactcaa ccattaacct tccccttatc caaggggagg gcagggtggg gttgttaaaa
     7321 acaagtaaag cttaatcaaa aaataaactt cccaagttgc tgtctggaaa aatcctaata
     7381 gatcatcgca aaattaaatt tatagtattc gcatttgctt taagtttaca tttattctcc
     7441 tgcaataaaa ctcccgaaaa gactgaaaag aaattttcta taaaagatga cgcaggtatt
     7501 gatgtagaat tttcatcata ccccgaaaga attatttcac tcggtccgaa tattactgaa
     7561 accatttatg cattaggtgc agatacatta ttatatggcg ttacggaatt ttgtgattat
     7621 ccacctgaag taaagacaaa agtcaataca ggcagctatc tcagtcctga ctatgaaatc
     7681 atttcttcac tcaaacccga tcttattata gtaaatgttg aatcttcaaa taatcctact
     7741 tatcagtctc ttaaaaatat gggttataac ttattcctaa gtcacgcaaa agacattgaa
     7801 ggaattttta aaatggtcaa tgatattggc atgatcacta acagggaaaa taatgctaat
     7861 atacttttta attctatttc ggatcagtta aaatataaag acccttcatc agttcaaaag
     7921 aaagataaaa cagtattgat actgattgca attaatccga tgatgacaac taacaagaat
     7981 acttttatta atgaaattgc attactcgca ggtctgaaga atatttattc agaagaagat
     8041 caggaatatc ctgtgataaa ttatgaagat gtggtcagca aaaatccatc tttcattatt
     8101 ttccctgctg atactcttga
//
LOCUS       DAORVQ010000154         8100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5097_length_8100_cov_6.000497, whole genome shotgun sequence.
ACCESSION   DAORVQ010000154 DAORVQ010000000
VERSION     DAORVQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5097_length_8100_cov_6.000497"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="PLX80_11215"
     CDS             <1..1924
                     /locus_tag="PLX80_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21402.1"
                     /translation="DSCEVPLSTSRTKINAYDIKFNCINFEMTRCASHIVNIIPVNVP
                     IPNFLSDRQVIFKGQSINFTDISTNSPTGWSWTFPGGTPSSSNVQNPQGIVYNSSGQF
                     NVTLQASNSFGNNSITKTNYITVLENPYSCPYTWLSKITATDAANRTDTVTFGMSPNG
                     TYGIDTCLGEYLLPPPPPLGIFDFRIVLPPTYNKDAVRSDFRQDLLSNSIYWLNFQTS
                     VSGPITFRWDPSLFPSTGTFYLREDILGTNINMKNQNSFVLSNAGIRFLRIDYEFSAN
                     YSIDVSSSWNLISIPLQAVDMSVSSLFETDVNTAFGYNNGYFNLSTLSNGNGYWVNFP
                     NARSYNVSGIFKYPKDINVISGWNMVGPFEEVIPVNEIVSYPSGLFASNFYGYNNGYL
                     TEDTLKPGKGYWIKTNSAGKFMKDTVGSLNQDFTNVQLQRADNWIRFEISDLNESGTT
                     LYLADQNEININHELPPVPPAGIFDARFLTDRSVEVFGIDQNIKINSAGQPLYFTASN
                     LKGNSIRIKDNIDGSVMNVLLKENERILLNSSLDNYLITTEKIMPNKYELAQNYPNPF
                     NPSTKINYSIPVTGKVNLIIYNALGQEVIKLVNEVQEAGSYSVKFNGADLSSGIYFYR
                     IESGSFKEIRSMILIK"
     gene            2119..4185
                     /locus_tag="PLX80_11220"
     CDS             2119..4185
                     /locus_tag="PLX80_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21403.1"
                     /translation="MKTLYTILFIVVICLAINKSAFSQANSVELQDGGGGLLSLHSSI
                     EEAYTAIPAVITQAYIIEILTIYTGANEVFPIDLRSKSGHSSSNTITIRPDAGNSGEV
                     ISGLSANGIINIHSADYIILDGRPGGAGSVPDLEVRNTGTTSNANTIALDSGATHCVI
                     KYIKSYNNTNGATGPRNIEIGTSPNIPGGCSNNLIDNCYVNGGRTGIGSDGGTAPSGN
                     ANVDNVISNCVIENFGFVAVWLLNFTGNTTVENNVINGTGTHSGIANVTAINIQSTVD
                     GYTINIRRNKITNFIANSTASTLAPRGIATITAPGTGSVLNIENNFISMTANNNNAAT
                     TYGIYTTGTSESYTCNIYYNTILIGGVQTGGLAGRIVSAPIVKQSTVPGVVYNQKNNI
                     CINTRTGGNAGVIHAGVGINGDAGTLDIDYNCYYSATGTPASWDSTNYATLQAYQNAI
                     GDYEENSRVKNVTFISSSDLHLDGASIQDPDLSARPVAGITEDIDGDTRSTAFPYKGA
                     DESTAFNLKTLNLKVNLEACSPVQDTVVVSLRNNFSPYSLVEMSKVYLSNTGTAAVNF
                     AKAVDGLNYYVVINHRNSIETWSKTGGEIFSGGVLNFDFTTMSSQAFGNNLVQVGSNW
                     SNYTGDVNQEGVVDVGDLTIIDNDAFVFITGYVPADLNCDDVVDLTDASYADNNAANF
                     VSVIKP"
     gene            4523..5773
                     /locus_tag="PLX80_11225"
     CDS             4523..5773
                     /locus_tag="PLX80_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21404.1"
                     /translation="MKFILFKIRNLSCLLLIVSSMTLLESCEESSVITSTKGTLAGKV
                     YSVSKFAMRNIEIKVGDKSVSTSDDGEFSLSDVEFPCNVILRNPGSNYATKYLNVTEG
                     TSTFIVEGVDSYTLNTKLKITLPSGIFDNSDNATMIFSDGMNVNLSKEFDSQTPPEEL
                     FVSYAGSNPVTGKLHVIIYSKENSKVVTFDNYAVSENIETVLGQEITYDFSMNQFDLN
                     PGEKTVNINIPVPPSGSYVGFNFLSFSKYKNINSQYPARQSFILQSPEVVIPLNIPGL
                     ISFAEIHTSGYVGKERLTEESSQSFQTKNDPQLISPENNAAGISGATEFSYTQSQGTG
                     IYFIDVYNSSTQERYTLVTKDNSFLFSEITEMAEISTSNKTYNYYVRKFGEAADIKDF
                     LNNYYNSEVSYRAGSDLRSFTTGE"
     gene            complement(6043..7695)
                     /locus_tag="PLX80_11230"
     CDS             complement(6043..7695)
                     /locus_tag="PLX80_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21405.1"
                     /translation="MKYSKIILIILFITGLHLKSNAQVSMQWSSFYNGASNNDDIPVA
                     MVIDNAGNTYVTGISRAVNMGDDFLTVKYNSAGVEQWTARYHNTSGNDDRVRAITVDN
                     SGNVYVTGSVSFGGANGIQVTIKYNSAGIEQWIRVLGEPVASPAAPGIYKSSIGTDAS
                     GNIYIGGYRKFGSADNGGMFLIKYNSNGDSLWTRRYKGTQFLQGLGSSITCIKVAGNF
                     IYVTGKSYDMNPNRTFATTIKYDSDGSAQWLSRDTLINGSDDVIGMESDASGNVIVAC
                     NYGFDIMMLKYNSAGNLIWRKNYAGVGGDHYDRVTALEIDASSNIYLTGNGVRAAGED
                     ADYLTLKFDPNGNLLWENFYNGNRNVDDYSRGIALDAAGNVYVTGLTYELPFNDNFMT
                     IKYNPAGVEQWRINYDGGFNNHHDEAVAIGVDSNGDIIVTGFANRQTTQDDFTTIKYS
                     QSVGISQVSSNVPDKYSLSQNYPNPFNPATVIEFRISKTEFVSMKIYDMQGKEIAAPV
                     SSVLSPGSYKYNFDASALSSGVYFYKLNAGDFSEVKKMSFVR"
BASE COUNT         2714 a         1351 c         1477 g         2558 t
ORIGIN      
        1 ggacagctgc gaagttccac tttcaacatc aagaacaaag atcaacgctt atgatataaa
       61 gtttaattgt ataaattttg aaatgacaag atgtgctagt catatagtaa atataattcc
      121 agttaatgta cctataccaa atttcttatc tgacaggcag gtcattttta aagggcagag
      181 tataaatttc acagatattt ctacaaactc tccaacgggc tggagctgga catttcccgg
      241 aggtacacct tcatcttcca atgttcaaaa tcctcagggt atagtttata attcatcagg
      301 tcaattcaat gttactctac aggcatccaa ttcattcgga aataattcca ttacgaaaac
      361 taattatatt actgtacttg agaatcccta ctcctgtcct tacacatggc tttcaaaaat
      421 aacagcaact gatgcagcaa acagaactga tacggttaca tttggaatgt ctccaaacgg
      481 cacttatgga attgatacat gtctgggtga atatttactt ccgcctccgc cgcctcttgg
      541 gatatttgat ttcagaattg ttcttcctcc tacttataat aaggatgcag tcaggagtga
      601 tttcagacag gatcttcttt ctaattctat atactggctg aactttcaga cctctgtatc
      661 cggaccgatc acattcagat gggatccgtc tttatttcct tctacaggta ctttttatct
      721 gagagaagat attctcggca caaacattaa tatgaaaaat caaaacagtt ttgtgctgtc
      781 aaatgcggga atcagatttc tcaggataga ttatgaattt agtgcaaact atagtattga
      841 tgtcagcagc agctggaatc tgatttcaat tcctctgcag gcagttgata tgtctgtttc
      901 ttcacttttt gaaacagatg tgaatacagc ttttggttat aataatggtt atttcaattt
      961 aagtacactg tcaaacggaa acggatattg ggtgaatttt ccaaatgcaa gatcttataa
     1021 cgtatcaggg attttcaaat atccgaaaga tataaatgta ataagcggct ggaatatggt
     1081 aggtcctttt gaagaagtaa tccctgtcaa tgaaatagtc tcatatcctt caggactttt
     1141 tgcttcaaat ttctatggct ataataatgg ttacctgacg gaagatacat taaagccggg
     1201 gaaaggatac tggataaaga caaacagtgc agggaagttt atgaaagata ctgtgggaag
     1261 tttaaatcag gattttacaa atgtacagtt gcagagagct gataactgga tcagatttga
     1321 aatttcagat ttaaatgaat cgggtacgac cttgtatctt gcagatcaga atgaaattaa
     1381 tattaatcat gaattaccgc ctgttcctcc tgcaggaatt tttgatgcaa gatttttgac
     1441 agacagaagt gtggaagtat ttgggattga tcagaatata aaaattaatt ctgcaggtca
     1501 gccgctttac tttaccgctt caaatttaaa agggaattcg atcagaataa aagataacat
     1561 tgatggttcg gtcatgaatg ttttactaaa ggagaatgaa agaattttat tgaatagttc
     1621 tctcgataat tatctgatca ccactgagaa gatcatgcca aataaatatg aacttgctca
     1681 gaattatcct aatccattta atccatcgac aaaaataaat tacagtattc cggttacggg
     1741 aaaagtaaat ctgattatat ataatgcttt aggtcaggaa gtgataaaac ttgtgaatga
     1801 agttcaggaa gccggaagtt acagtgtgaa atttaatggt gcagatcttt cttcgggtat
     1861 ttatttttac aggattgaat ccggtagttt taaagagatc aggtctatga ttttgataaa
     1921 gtaattatat taggaaaatt aaactgcagc tgagaatgac tttgcgagga gcgaagcgac
     1981 gaagcaatct ccaaatctaa tgcagtattt tctttggatg ggggcttaat cctgtttgaa
     2041 ttttaaaatt atatttgaaa ataataaaat tttgtttaat tttattaaat ccattttccc
     2101 aaaattaaat ttaaaaaaat gaaaacactt tacacaattt tgtttattgt tgttatttgt
     2161 ctggcaataa acaaatcagc attttcacag gcaaattcag tagagctgca agacggcggc
     2221 ggcggattac tgagtttaca cagcagcatt gaagaagctt atactgcaat acccgcagta
     2281 ataacgcagg catatattat tgagatactt actatttata ccggtgcaaa tgaagttttt
     2341 ccaattgatt taagatctaa gtccggacat agttcatcta acactattac aattcgtccg
     2401 gatgcaggca attccggaga agtaatttcc ggtttaagtg ccaacggaat tatcaatata
     2461 cattcagccg attatataat attagacggc agaccgggtg gtgccggaag cgtgcctgat
     2521 ctggaagtca gaaataccgg aactacatct aatgctaata ctatagctct tgacagcgga
     2581 gctacacatt gcgtcattaa atatataaaa tcatataaca atacaaatgg agctaccgga
     2641 ccgagaaata tagaaatagg tacttcacca aatattccgg gcggatgctc aaataatctt
     2701 atagataatt gctatgtaaa cggcggcaga acaggtatcg gaagcgacgg gggaacagct
     2761 ccgtccggaa atgcaaacgt ggacaatgta atttcaaatt gtgtaatcga gaattttggt
     2821 tttgttgctg tctggctttt gaattttacc ggcaatacaa ctgtcgaaaa taatgttata
     2881 aacggcactg gaactcacag tggaattgca aatgtgactg ccataaatat acagagtaca
     2941 gttgacggat atacaattaa cataaggaga aataaaatta caaacttcat tgcaaactct
     3001 actgcatcaa ctttagctcc gagaggaatt gcaacgatca ctgcaccggg taccgggtcg
     3061 gttttgaata tcgaaaataa ttttatttca atgactgcaa ataataataa tgcagctaca
     3121 acttatggaa tttatacaac cggtacttct gaaagctata catgtaatat ttattataat
     3181 acaatattga taggcggggt acagacaggt ggcttagccg gaaggattgt ttcagcaccg
     3241 atagtgaaac agtcaactgt acccggagtt gtttataatc agaaaaataa catttgcata
     3301 aatacaagaa ccggaggaaa tgcaggagtt atacatgccg gagtcggaat aaacggagat
     3361 gcaggtacac tggatataga ttacaattgt tattattcag caaccggaac gccggcttca
     3421 tgggattcaa caaattatgc tacacttcag gcatatcaga atgcgatagg tgattatgaa
     3481 gaaaattcaa gagtcaaaaa cgttacattc atttcaagtt cagatctcca ccttgacggc
     3541 gcatctattc aggatcctga tctcagcgcg agaccggttg ccggaattac cgaagatata
     3601 gacggtgata caaggagtac agcattcccg tataaaggag cagatgaatc tacagcattt
     3661 aatttaaaaa cattaaactt aaaagtaaat ctcgaagcat gctctcctgt gcaagataca
     3721 gtagtggtat ctctaagaaa taatttttct ccatacagtt tagtagaaat gtcaaaggta
     3781 tatttaagta ataccggaac tgctgcagta aactttgcaa aagcagtgga cggattaaat
     3841 tattatgtag tgataaatca cagaaattcg atagaaacat ggagtaaaac gggtggagaa
     3901 attttttcag gcggtgtatt gaattttgat tttacgacaa tgtcatcgca ggcattcgga
     3961 aataatcttg tacaggtagg cagtaactgg tctaattata ccggtgatgt aaatcaggaa
     4021 ggcgtagtag atgtaggtga tctgacaatt atagataatg atgcttttgt atttattaca
     4081 ggttatgtac cggcggattt aaattgtgat gatgtagtag atctgacaga tgcatcttat
     4141 gcagataaca atgctgcgaa ttttgtttct gttattaagc catagaaaag gatgtgagaa
     4201 ttttttaatc gaaaactaaa ttaaaataga gacaagggag taatactccc ttgttttatt
     4261 tagtgtaaat tgagcagatg tattgttagt ttgttttaat catttatagt attggcggta
     4321 ttattgaaag tataccaatt ggtttgcata gaataatcat tttttgaatt caaattttca
     4381 gttcaccaaa gagcttcgaa catttgcagg attgatgagt gggtttgctt ttgatagttt
     4441 aataagtttt aattaaacaa aaaacggtcg tgcagtttaa gattaaacca attagtttat
     4501 catacaatct aatttataaa aaatgaaatt tatacttttt aagatcagaa atttgtcatg
     4561 cttgttactt attgtatcat ctatgacatt gctggaaagt tgtgaagaaa gttcggtcat
     4621 cacttctact aaaggaacat tagcagggaa agtttattcc gtctctaaat tcgctatgcg
     4681 gaatattgaa attaaggttg gtgataaatc ggttagtact tctgatgacg gtgaattttc
     4741 attgagtgat gttgagtttc catgcaatgt aattctgaga aatccaggtt caaactacgc
     4801 aacaaaatac ttaaatgtaa ctgaaggtac ttctacattt attgttgaag gagtggattc
     4861 atatacatta aatacaaagc tgaagattac actaccttcc ggaattttcg ataactccga
     4921 taatgctact atgatctttt cagatggaat gaatgtaaat ctttccaaag aatttgattc
     4981 acaaactcca ccggaagaat tatttgtttc ttatgcagga agtaatcccg tgacaggcaa
     5041 gttacatgta attatctaca gcaaggaaaa ttcaaaagtc gtaacatttg ataattatgc
     5101 tgtcagtgaa aatatagaaa cggtgttagg acaggagatc acttatgatt tttcaatgaa
     5161 tcagtttgat ctgaatccgg gtgaaaagac tgtaaacata aatatacctg ttcctccaag
     5221 cggatcatat gttggattta attttctttc tttttctaaa tataaaaata tcaattcaca
     5281 atatccggca cgtcagagtt ttattcttca atctcctgaa gttgtaatac ctttgaatat
     5341 tccgggactt atcagctttg cagaaataca tacaagcggc tatgtcggaa aggaacgatt
     5401 aactgaggag tccagtcaaa gttttcaaac aaagaatgat cctcagctta tttcacctga
     5461 aaataatgct gcaggaatca gcggtgcaac ggaattttct tacactcaat ctcagggtac
     5521 agggatttat tttattgatg tttacaattc gtccactcag gagagatata ctttagttac
     5581 aaaggataat tcatttttgt tcagtgagat cacggaaatg gcagagattt caacttcgaa
     5641 taaaacttac aactactacg tcagaaaatt tggtgaggca gcagacatta aagatttcct
     5701 gaataattat tataacagcg aagtatcata tcgagccggt tcagatctca gaagttttac
     5761 aacaggggag tgaatatttt ataataagga agtcccggaa attaaaattt tatctataga
     5821 agccctaact gaaattcccg catcatgacc tgatctaata gaaaatgcag tatcaaatgc
     5881 tttagatgat attgcttttg taaaagcgac cgaagaagtt gataaaagga atatgttagc
     5941 tatgatgaac ttatgaaaat gatggacaaa ataattatca gttaggaaaa taaataatga
     6001 atattcatgc tgaaaaaaca atttccaagt gacataatat attcagcgta caaagctcat
     6061 cttctttact tcagaaaaat caccagcatt taatttataa aaataaactc cgcttgaaag
     6121 tgctgaagca tcaaaattat atttgtaact tccaggtgat aaaactgaac tgaccggtgc
     6181 tgcgatttct tttccctgca tgtcataaat tttcattgat acaaattctg ttttcgaaat
     6241 tctgaattca atgacggtag ctggattaaa gggatttgga taattttgtg acagagaata
     6301 tttgtcagga acatttgatg aaacctgtga aattccgaca gattgagaat attttatggt
     6361 tgtaaaatca tcctgcgtag tctgtctgtt ggcaaatcct gtaactatta tatctccgtt
     6421 tgaatccact ccaattgcaa ctgcttcatc gtgatgatta ttaaatcctc cgtcataatt
     6481 tattctccat tgttccacac ctgcaggatt gtatttaatt gtcataaaat tatcattgaa
     6541 aggaagttca taggtcagac cggtaacata tacatttccg gcggcatcaa gagcaattcc
     6601 tcttgaataa tcatcaacgt ttctgttgcc gttataaaaa ttttcccaaa gcaaatttcc
     6661 attcggatcg aatttcaaag taagataatc tgcatcttct cctgctgctc tgacaccgtt
     6721 tcctgtaaga taaatattag atgaagcatc aatttccaat gctgtcacac ggtcataatg
     6781 atcaccgcct actccggcat aattttttct ccatattaaa ttcccggcag aattatattt
     6841 cagcatcata atatcaaatc cataattaca tgcgacgatc acattacctg aagcatcaga
     6901 ttccattcca attacatcat cactgccatt gatcaaagtg tccctgctaa gccattgtgc
     6961 agaaccgtcg gaatcgtatt ttatagtagt tgcaaatgtt ctgttcggat tcatatcata
     7021 tgactttcct gttacataga taaaattacc tgcaactttt atgcatgtga tcgaacttcc
     7081 cagtccctgc agaaactgtg ttcctttata tctccttgtc cagagagaat ctccgtttga
     7141 attatatttt atcaaaaaca ttcctccgtt atccgcactg ccaaattttc tatagccgcc
     7201 gatgtaaata tttcctgaag catctgttcc gattgagctt ttataaattc ccggagctgc
     7261 aggacttgca acgggctcac ctagcactct gatccactgc tcaatacctg cggaattata
     7321 ttttatagta acctgaattc cgtttgctcc gccgaaactg acagaacctg taacataaac
     7381 atttccagaa ttatcaactg tgattgctct tacccggtcg tcattaccgg aagtattatg
     7441 ataccgtgca gtccactgtt cgacgcctgc tgaattatat ttcaccgtga gaaaatcgtc
     7501 tcccatattt actgcccgac tgataccggt tacataagta ttgccggcat tatcaatgac
     7561 cattgcaaca ggaatatcat cgttgtttga tgctccgtta taaaagctgc tccactgcat
     7621 acttacctgc gcattggatt tcaaatgcag tccagtgatg aatagaatta ttaaaataat
     7681 tttagaatat ttcataataa gttttaatag ttatttaatt aaattttctt tacaaaatta
     7741 ttaaaaataa ggaaggaaat acaagtaaag attatccgga ttcttacaaa atttgaatag
     7801 ataaaagtga gaaaagttta tttttaaaca aaaagtagag tttaatgaga tatatattta
     7861 caatttttaa taataatttt tcagattatg cggtaaataa ttatacagaa acaattaaat
     7921 ttcaatttct ttttacaaag taaattttaa ttttgtaaaa attaatattt aatatctaat
     7981 atttattatt aaatgaaaga ccactcatta ataaaattat taagaacttt ttcgggtgaa
     8041 gagattaagg aattcggaga gttcatctca aattcatatt acaataaaag gaaggcagta
//
LOCUS       DAORVQ010000155         8099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5098_length_8099_cov_5.052586, whole genome shotgun sequence.
ACCESSION   DAORVQ010000155 DAORVQ010000000
VERSION     DAORVQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5098_length_8099_cov_5.052586"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1138
                     /locus_tag="PLX80_11235"
     CDS             407..1138
                     /locus_tag="PLX80_11235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21406.1"
                     /translation="MNQVLEEILKTGFTQKLDGSPHKVIGQISEEEGLLIQECAKECR
                     ANTSVEIGLAFGTSALFICESLRKNKNTKHTIIDPYQNFDHSYGGIGLNNLKRAGFEN
                     IVRFIEKPSHLGLAQLESEKVMVDLAFVDGWHTFDHVIVDFFMVDKILHEGGIVIMDD
                     SDWPAIEKVCSFISSNRSYEFIRGTSPRTTSNGKEKNVNNKRITEKFKNVINKTKTQK
                     KQYGAMAFRKISHDKRSWDHFIDFL"
     gene            1197..2801
                     /locus_tag="PLX80_11240"
     CDS             1197..2801
                     /locus_tag="PLX80_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HQY21407.1"
                     /translation="MNKFKIFSILIILPVLLFITAKSLRNSEGPFYFNAGYDPNYVYA
                     VSSLNIINSVHVSHTDHPGSTVQLIGAGLMLLSGKSVSEITESVLLNPESYLNLFNTV
                     LILLNSLCLLLCGLVIYHVTKNLILTLIIQLSPFLSQTLIHEFTQTNPEIFIMPMMML
                     FIAVLFSYREYIINSDKFSLKYPLMFGILSGVCLATKITFLPLMIIPLFILKGSRSKM
                     IFFTTSVLSFITITYPVMGSYEHFLNFIGNLSTHDGLYGRGEKIIFNFDTIYKNFESL
                     IIHDMFFVLILIIGILLFIYYKFKRTDYSKIFTIDQNILLSLLVTFIFQIFIVSKHYS
                     QHYLVPALMLTSFAVIIICINFKEIGLTNYFKFTNKIYNHILLICITVSIFNLYIVFN
                     DSTEYSSETQSLINIMDNGNDNQFVFNSYGTSDSNYGMMFSMNWAGEMKEHYTSVLKS
                     KSSNNFNVDFWNSEITYYENFENIKINLANSPDIFLRLKCFDFNKNAVSDFKKNIIQI
                     TGNERYNFSELKRNKSGESVYRFSVK"
     gene            2862..3329
                     /locus_tag="PLX80_11245"
     CDS             2862..3329
                     /locus_tag="PLX80_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21408.1"
                     /translation="MCLKLKLRFKLTSGKLSLSKILKISGALTDLLKSLCTRQKNELK
                     NENLTQYYYFKSHPPKRLSVKLDICIFYCSFLNDQKRTKKIAAGDKLPEILSHGYKNI
                     NSSPDRKALSGSSNNIFLLNAIAQNFLSAIYHRRVILNKLPEKIQMRDSFHLN"
     gene            3448..3732
                     /locus_tag="PLX80_11250"
     CDS             3448..3732
                     /locus_tag="PLX80_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY21409.1"
                     /translation="MLDSSSIPKEALSVYKQRWTIENLFGNMKTKGFNIEVTQMKDDE
                     KLKKSTALLTIAVLWYFLLDLWIESSVKIRIKNHGRKEKAHSEKDRIIFQ"
     gene            4009..5424
                     /locus_tag="PLX80_11255"
     CDS             4009..5424
                     /locus_tag="PLX80_11255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21410.1"
                     /translation="MSKRKDNVIRHKNDIASDRKKWIRKNKYYYKNLTKLLKFSIPEG
                     SSIIEIGCGIGNLINDLKPSRGVGIDISDKMIAIAKTEYPQYEFIVMDSEEISITEKF
                     DFIIISDTVGYFDDVQRSFEQIQNLCSEHTRIIVTYVNFLWLPVLDLAELLRLKMPQV
                     RNNWLNIDDLKNLLNLSGYDVIKSGRKFLMPFYIPLLSAFVNNYIANLPLINRLCLIE
                     FIISRKIINEVQEKTVSVVIPARNEKGNIETIIQRVPQMGSNTEIIFVEGNSTDDTYD
                     EIKRVCEKYSQSHDLKYFKQEGKGKADAVRKGFENVNGDILMILDADMTVPPEDLTKF
                     YNAFVQRKGEYLSGTRLVYPLEKESMRTLNILGNKFFSLMLTWILNQKIKDTLCGTKV
                     ISRENYIKLKEVKNNFLGNADPFGDFDLILGASKLNLKYAEIPIRYDARIYGETNISR
                     FSHGWMLMRLTLFAMNKFKFI"
     gene            5427..6155
                     /locus_tag="PLX80_11260"
     CDS             5427..6155
                     /locus_tag="PLX80_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21411.1"
                     /translation="MEDIYLKHRKVWNEKKLLREIYSEWYQKIINDLSLSVGKTLELG
                     GGGGNFKEFKPDVISADIVPLDWLDMCFDAHEMPFKENEISNIVMIDVLHHLNDPIKF
                     LNESYRVLNTGSRIIMIEPFPSPFSLMIYKKFHPEPFDKTVDYFSEFENNSDKDPWDS
                     NQAIPYLLFYKFRNKFNELFGNKFKFIKKEKFSFVLYPASGGFENKQMIPDNMTGVVK
                     LIEKSLNPLKDLLAFRCYIVLEKI"
     gene            6182..7771
                     /locus_tag="PLX80_11265"
     CDS             6182..7771
                     /locus_tag="PLX80_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21412.1"
                     /translation="MLILPVLLFIAAGLLKYAQGPYYLNFYDPSYVYLINGLNLAQLN
                     GYGVGHFDHPGTTVQVISAVVIKFYYAVSPADTDIISDVFSRPEEYLLKINVFLNAVN
                     CVLLFLSGYFLYFRSKNIYLTLLIMLSPFTSTELFYGMIIVTPDNLLISASLLIIGMI
                     FYCLYKKDQEYNIKEIIVFAAIIAFGLATKLNFIPMILIPMIILNGIKKKLLFLTFTL
                     IFFVIFILPGISNIEYFLKWVEGLILREGKHGKGAANIINASSFSKNLFTIFKTDILF
                     TISYFTLLITYAIVLYKNYVTKNIRSVFRTPVLYRLLAGILTAMTLQLIIVSKHYAQY
                     YMIPSFMLTITGLYISYLVIFEISGDKFRTARTKIIYIFSSIVLVIIISYQIISSYYE
                     GTEQKKDAFTIMNFIEEKYPASIIIPSFGSANTEAPLAFASQYAGSKKEDYKNLLNEQ
                     LRSDVFFNQWINEFYILSEEGRSADYFSGNKKIYLQLNKYGSIEDFILKLKSLTGKNN
                     ISYNVIFTNEKKESVYEIFLR"
BASE COUNT         2835 a         1254 c         1272 g         2738 t
ORIGIN      
        1 ccctcatttg aattttctcc ggtaacttat ttagaataat ccgcctgtga taaccttttg
       61 tgcttccggg attgtctcaa atcttcttac cgccaagaca ccaagacacc aagacacaaa
      121 agaaaaaagt taataaatat ttatctttgt gacttagtgt ctttgtggtg aaattatata
      181 ctgttaatag acagactctt ccaaagtggg atcgttcaaa aaagaacagc agaaaataca
      241 aatatcaaat ttaactgata atcgttttgg acagagtgac ctgtaattca atttaccatt
      301 taaaaaagga ttgaaaaaca attaagactg acttaactta catgcattac aaaattgcac
      361 atttttaatt tatcacataa gaaatttatt tattatatta cactaaatga atcaggtact
      421 tgaagaaatt ttaaaaacag gtttcacaca aaaattagat gggagtccac ataaagtaat
      481 tgggcagatc agtgaagaag aaggacttct cattcaggaa tgtgctaaag aatgcagagc
      541 aaatacaagc gttgaaatag gattagcatt tggaacttca gctttattta tctgtgaatc
      601 actcagaaag aataagaata caaaacatac catcattgac ccttatcaga attttgatca
      661 cagctatggc ggtatcggac tgaataattt aaaaagagca ggatttgaaa atatagttcg
      721 ctttatagaa aagccgtcac atcttggcct ggcacagctt gaatccgaaa aagtaatggt
      781 agatcttgct tttgtagacg gctggcatac atttgatcat gtgattgtag atttttttat
      841 ggttgataag atccttcatg aaggcggaat cgtaattatg gatgattcgg actggccggc
      901 tatcgagaaa gtatgttcct ttatttcttc aaacagatca tatgaattca tcagaggtac
      961 ttcaccaagg acaacatcca acggaaaaga aaaaaatgta aacaataaaa ggataactga
     1021 aaagtttaag aatgtaataa ataaaaccaa aacccaaaag aaacaatacg gagcgatggc
     1081 ttttagaaag atctcccatg ataagagaag ctgggatcac ttcattgatt ttctgtgata
     1141 taatagattt tttgctttgt atataacaat ttaaattatt ctttattcaa ccccaaatga
     1201 ataaatttaa aattttttca atattaatta tcctgccggt attattattt attaccgcta
     1261 aatcattaag aaattccgaa ggtccatttt actttaatgc agggtatgac ccgaattatg
     1321 tatatgccgt cagctcttta aacattatta attctgtgca tgtttcacat acagaccatc
     1381 ccggatctac tgttcagttg ataggtgcag gtctgatgtt actatccggt aaatcagttt
     1441 cagagattac agaatctgta cttttaaatc ctgaatctta tttaaattta tttaatacag
     1501 tcttaatttt attaaactct ttgtgtctgc ttttatgcgg acttgtaatt taccatgtta
     1561 caaaaaatct gatactcaca ttaattattc agctctctcc atttctatct caaactctta
     1621 tacatgaatt tactcagaca aatcctgaaa tttttataat gccaatgatg atgttgttta
     1681 ttgcagtact gttttcatac agagaatata tcatcaatag tgataaattc agtttgaaat
     1741 atcccttaat gtttggaatc ttatcaggtg tctgtcttgc cacaaagatc actttcttac
     1801 ctctgatgat tatccctctt tttattctaa aaggatcaag aagtaaaatg atttttttta
     1861 caacttctgt attatccttt ataactatta cctaccctgt catgggatcc tatgaacatt
     1921 ttttaaactt tattgggaat ctgagtacgc atgatggact gtatggcaga ggggaaaaaa
     1981 tcatttttaa ttttgatact atttataaaa actttgaaag tcttatcatt cacgatatgt
     2041 tttttgtatt aattctaata ataggaattc tgctctttat atattataaa tttaaaagaa
     2101 ctgactacag taaaattttt actattgatc aaaatattct gctgtcttta ttagttactt
     2161 ttatttttca aatttttatt gtttccaaac attacagtca gcattattta gtaccggctc
     2221 taatgctgac ttcttttgca gttataatta tctgcattaa tttcaaagaa attggactta
     2281 ccaattattt taaatttaca aataaaattt ataatcatat cctgttaata tgtatcactg
     2341 tttcaatttt caatttatat attgttttta atgactcaac tgaatattcc agtgagacac
     2401 aatcattaat aaatattatg gacaacggta atgataatca atttgtattt aacagttatg
     2461 gtacttcgga ctctaattac ggaatgatgt tcagtatgaa ctgggccgga gaaatgaaag
     2521 agcactatac ttctgtttta aagagtaaat cttcaaacaa ttttaatgtc gatttctgga
     2581 attctgaaat tacttattat gaaaattttg aaaacataaa aataaattta gcaaactcac
     2641 ctgacatctt tctcaggtta aaatgtttcg attttaataa aaatgctgtt tcagatttta
     2701 aaaaaaatat cattcagatt acaggaaatg aacgttataa tttcagtgaa ttaaaacgca
     2761 ataaatcagg agaatctgtt tacaggtttt ccgtaaagta atccgttttt aaattctttt
     2821 tagatctgta ttccagcctg tatccggact ccggcttgag tatgtgcctg aaactgaaac
     2881 tccgtttcaa actaacttcc ggcaaattat cactttcaaa gatattaaaa atttccggag
     2941 ctttaacaga tttgttaaaa agcctatgta cacgacaaaa aaatgaatta aaaaatgaaa
     3001 atctgaccca atattattat tttaaatcac atcctccaaa acgtttatca gtaaaattag
     3061 atatttgtat tttctactgt tcttttttga acgatcaaaa aagaaccaaa aaaatcgccg
     3121 ctggtgataa attgccggaa attctttcgc atggctacaa aaatataaac tcctcacctg
     3181 acagaaaggc actgtcaggt tcgtcaaaca atatattttt gcttaacgcc attgctcaga
     3241 atttcttatc ggcaatttat cacaggcggg ttattctaaa taagttacca gagaaaattc
     3301 aaatgcggga tagttttcat ttaaattaat aaattgtatt tttcaataat tttgaactat
     3361 ttatttaaaa tttaaaatgt tttggacagc actgattcta aataatccac tattaaaaaa
     3421 taatattgaa aggatcagat ttatttaatc ctagacagca gcagcattcc caaagaggca
     3481 ttatctgtgt ataagcagag atggacaata gaaaatcttt ttggaaacat gaaaacaaaa
     3541 ggatttaata tagaggttac gcaaatgaaa gatgatgaga aattaaaaaa atcaacagca
     3601 cttttaacaa ttgcagtact ttggtatttt ctgttagatc tttggataga aagttcagtt
     3661 aaaatcagaa ttaagaacca cggcagaaaa gaaaaagcac attcagaaaa ggaccggatc
     3721 atttttcaat aaaattctaa atacatttga aaaaatgatc tttgaaatag attgtgtgtt
     3781 taaagttctg tggtgtaaat agttaaaaag taaagtaaaa cattcttaat ttcttattaa
     3841 atctgcatta gttagtttgt aacattcaaa taatattcta cagacataaa ttatttcctg
     3901 tctgtttttt aataaaattt tttatttgac agctaattac atattcaatt ctatagatct
     3961 tttttctgtc ctgatctgtc tcaatagatc atttaaaatc cagtctttat gagcaaacga
     4021 aaagataacg tgatccgtca taaaaacgat atcgcatctg acagaaaaaa atggatccga
     4081 aaaaataaat attattataa aaatttaaca aagcttctga aattcagcat tccggaagga
     4141 agcagcatta ttgaaatagg ttgtggtatt gggaatctga ttaatgatct taagccttcc
     4201 agaggagtcg gtatcgatat ttcagataaa atgatcgcga ttgcaaaaac tgaatatccg
     4261 cagtatgaat ttattgtaat ggattcagaa gaaattagta tcacagaaaa atttgatttt
     4321 ataataatat cagatacagt aggatacttt gatgatgtgc agagatcatt tgaacagatc
     4381 caaaatctct gcagtgagca tacaagaatt attgtgactt atgttaattt tctctggctg
     4441 cctgtactgg atctcgcaga gttactcaga ttaaaaatgc ctcaggttag gaataactgg
     4501 cttaatattg atgatttaaa aaatctgctc aatctctccg gatatgatgt gatcaaaagc
     4561 ggcaggaaat ttctgatgcc tttttatatt ccccttctgt ctgcatttgt gaataactac
     4621 attgcaaatc ttcctttgat aaacaggtta tgcctgatag aatttataat ttccagaaag
     4681 ataataaatg aagttcagga aaaaactgtc agtgtagtaa taccggccag aaatgaaaaa
     4741 ggaaatattg aaaccattat acaaagagtg cctcaaatgg gaagcaatac tgaaattatt
     4801 tttgtagaag gaaattctac cgatgatact tatgatgaaa taaaaagagt gtgtgaaaaa
     4861 tattcgcaaa gtcatgatct taaatatttt aagcaggaag gtaaagggaa agccgatgct
     4921 gtcagaaaag ggtttgagaa tgtgaatgga gatattctta tgatccttga tgcagacatg
     4981 acagtgccgc ctgaggatct gacaaaattt tataatgcat ttgttcagag aaaaggtgaa
     5041 tacctcagcg gtacaagatt agtttatcct cttgaaaagg aatccatgag gactctgaat
     5101 attctgggca ataaattctt ttcacttatg ctcacatgga tattaaatca aaaaatcaaa
     5161 gacactctct gcggaacaaa agttatttca cgggaaaatt atataaagct gaaagaagta
     5221 aaaaataatt ttttaggaaa tgcagatccg tttggagatt ttgatctgat actcggtgcc
     5281 tcaaagctca atctaaagta tgccgaaatt ccaatcagat acgatgcgag aatttatgga
     5341 gaaacgaaca tctcaagatt cagtcacgga tggatgctga tgagacttac tttatttgca
     5401 atgaataaat ttaaatttat ctgactttgg aagatattta tttaaaacac agaaaagtct
     5461 ggaacgaaaa aaaacttctt cgtgaaatat attctgagtg gtatcagaaa ataataaatg
     5521 atcttagttt atctgttgga aaaacacttg agcttggcgg aggcggcggt aattttaaag
     5581 aatttaaacc tgatgtaatc tctgctgaca ttgttccgct cgattggctg gacatgtgtt
     5641 ttgatgcaca tgaaatgcca ttcaaagaaa atgaaatttc aaatattgtg atgatcgatg
     5701 tacttcatca tctaaacgac cctataaaat ttttaaatga atcttacaga gtattaaata
     5761 caggcagcag gattattatg atagaaccgt ttccgtctcc tttttcactt atgatctata
     5821 aaaaatttca tcctgagcca tttgataaaa cagtagatta ttttagtgaa tttgaaaaca
     5881 attcggacaa agatccatgg gattcaaatc aggcaattcc atatttgctg ttttataaat
     5941 ttagaaataa atttaatgaa ctgtttggaa ataaatttaa atttataaaa aaggaaaaat
     6001 tcagttttgt attataccct gcatccggag gttttgaaaa caaacaaatg atccctgata
     6061 atatgacggg tgttgttaag cttatcgaaa agtctctgaa tcctttaaaa gatcttcttg
     6121 cattcagatg ttatattgtt cttgaaaaaa tatgatcagt aagaaatatt taattccgat
     6181 tttgttgatt cttccggttt tactttttat agcagccggt ttactgaagt atgctcaggg
     6241 tccgtattat ctgaattttt atgatccgag ttatgtgtat ctgataaatg gtttaaatct
     6301 tgcccagctc aatggttatg gcgtcggaca ttttgaccat ccgggcacta cagttcaggt
     6361 gatctctgca gttgttataa aattttatta tgctgtatcc cctgcagata cggatataat
     6421 ttctgatgta ttttccagac ctgaagaata tcttttaaaa attaatgtct tccttaacgc
     6481 agtaaattgt gtcctgcttt ttctgtcagg atatttttta tatttccgct caaagaatat
     6541 ttatctgaca ctactgataa tgctttcacc ttttacttct acagaattat tctacggaat
     6601 gataatcgta actcccgaca accttctgat ttctgcatcg cttcttataa taggaatgat
     6661 cttttactgt ctttacaaaa aagatcagga atataatatc aaagaaatta tagtattcgc
     6721 tgctatcatt gcattcggtc ttgcaacaaa attgaatttc attccaatga tcctgatccc
     6781 tatgataata ctgaatggta ttaaaaaaaa gttgctgttt ctgactttca ctctaatatt
     6841 ttttgtgata tttattctgc cgggtatttc aaatatagaa tattttttaa aatgggtaga
     6901 agggctgata ttacgggaag gcaaacacgg aaaaggcgca gctaatataa tcaatgcgtc
     6961 ttcattttca aaaaacctat tcactatttt caaaactgat attttattta caatatcata
     7021 cttcacactt ctgataactt atgcaatagt tctctataaa aattatgtaa ctaaaaatat
     7081 tagatctgtt ttcagaacgc ctgtacttta ccgcttactt gccgggattc ttacggcaat
     7141 gacactacag ctgataattg tatcaaaaca ctacgcacag tattatatga tcccctcttt
     7201 tatgctgaca ataacaggct tatacatttc atatcttgta atttttgaga tctcaggcga
     7261 caaattcaga actgccagga caaaaataat ttatattttt tcttcaattg tactggtcat
     7321 aataatttct tatcagataa tctcatctta ttatgaaggc actgaacaaa agaaagatgc
     7381 ttttacaatc atgaatttta ttgaagagaa atatccggct tcgatcatta taccttcatt
     7441 tggaagtgct aatactgaag ctccattggc atttgcctca caatatgcag gcagtaagaa
     7501 agaagattat aaaaatttat taaatgagca gcttaggtct gatgtatttt ttaatcaatg
     7561 gatcaatgaa ttttatattc tttctgaaga aggaaggtct gcagattatt tttccgggaa
     7621 taaaaaaata tatcttcaat tgaataagta tggttcaatt gaagatttca tattaaagct
     7681 aaaaagtttg accggcaaaa ataatatcag ctataatgta atatttacaa atgaaaaaaa
     7741 ggaaagtgta tatgagatat ttcttcgata aatacagaga ttattccgcg tcatttcaat
     7801 cgtttcagta caaaattcaa aatacattca tttctgctta cttttttccc cgcaaaaaaa
     7861 agtaacaaaa aaagtttccc gccggtgata aactgcctga aattctttct tcccgagtac
     7921 ccgggacagc agaaatataa actcctcatc tgacagaaag acactgtcag gtttgccaaa
     7981 caataaattt ttgtttaaag ccattgctca gaatttctta tcggcaattc atccggacag
     8041 acattcaata tcatttataa tattacctgt tcaattcttt cttttatttc agaaatttt
//
LOCUS       DAORVQ010000156         8098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5099_length_8098_cov_7.510506, whole genome shotgun sequence.
ACCESSION   DAORVQ010000156 DAORVQ010000000
VERSION     DAORVQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5099_length_8098_cov_7.510506"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    117..216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            605..2524
                     /locus_tag="PLX80_11270"
     CDS             605..2524
                     /locus_tag="PLX80_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21413.1"
                     /translation="MKSKVYIYSLLVVIIAAIYGFTSMDPITKDLTQRNSSFNNPMYT
                     YGAQLENPISTLNESFESATFPPTGWTKINPDAGTGWDRQTVGTTPLPGWNGGVITSP
                     PGGGIGVAFATWTTGGTVSNNQWLVTSQLTNSTVNDSLSFWTRFWPSNIYSDTVDIKI
                     STTTPTIGGFTTNVATIVFPRNSPDTNWTQHKYAIGSMIPAGSNYYIAFRERVLDNLN
                     DGASVSLDLVSVTTSTGPPACNYQWSSQTSGSTGQFFSVSAVSDMVCWAAGATATVRK
                     TTNGGSTWENGNSSPGVIAGDIYNIYAWSANDAICTTSPAASNIYKTTNGGATWTLSH
                     TVAGGFINALQMVSATEGYATGDPVGGIWEFLKTTDGGTTWAQVPTAPAQVGTEAGWN
                     NSFSIIGTNIWWGTNSTKVYRSTDMGATWSSGVSTGTVNTYALHYNNPTTGIAGGTAT
                     VVSANGGTSYTAATSPGTSGNINGFEGVGTDWWGIRSGATVYRSTNAAATWTDAYTQT
                     GAVYQDIDFVIVNGCPVGWAVGNLGVVSKMSNVTGISNIGGEVPVDYTLGQNYPNPFN
                     PETNINFTIPKSGNVTLKIYDVSGKEVSTLVNEVKNAGNYIVGFNAASLPSGAYFYRL
                     ETNGFSATKKMMLIK"
     gene            2806..4899
                     /locus_tag="PLX80_11275"
     CDS             2806..4899
                     /locus_tag="PLX80_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21414.1"
                     /translation="MKNKLHFYFIPLLLILFMVSGFSDKEIHQNLKDTEFKIQNNSGS
                     DTIPPANFPYPVVYNWYYQNIPGSNFETVGATYFQDKYVLNRWDNSTLYRYNKNGPGG
                     GPGSLSDSNNSYAGQIRDMTVAPDGSGQSFLWGGRASTVLYKMDANGNNIKQFSHTGA
                     AYRAIAWDPNRKGFWSCNFSDNIVCRDTNGVVLATLPNTLTGKYGMGFDSTSSVDSAF
                     LWVWSQGGTGLPNTLQKIHILSNSVAADYTFQLANGTDGIAGGAEVFIKDNQLILSLN
                     FQEFAIVGYKLKDIGPPPPVCNLIWFPQTSGTVNLLYSVSAVSEQICWAGGAAGTVRK
                     TTNGGATWADGNSTPGTIAGDIYNIYAWSANDALCTTSPAASNIYKTTNGGTTWTQVF
                     TLPGGFINAIEMVSPTEGYAEGDPVGGVWTILKTTDAGSSWTQMPTAPAQVGGEAGWN
                     NSFTARGSNIWFGTNSTRVYRSTDLGLTWTSGATTGTLNTYSLHFNNPNAGMAGGNAM
                     VLSNDGGANYSSIPSPGVSGNINSIEGHGSNWWATRSGADIYRSSNNGISWNTAYTQA
                     VTTFNDMDFIEVSGCPYGWAVGSSGAIAKMNFPVGISNTGGEVPVDYILGQNYPNPFN
                     PETNIKFTIPKSGIVTLKIYDVSGKEVSTLVNEVKNAGNYIAGFNAGDLPSGAYFYRL
                     ESEGFSASKKMMLIK"
     gene            5298..6521
                     /locus_tag="PLX80_11280"
     CDS             5298..6521
                     /locus_tag="PLX80_11280"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQY21415.1"
                     /translation="MSDKKLSLQKLIDNNEIAGKRVLVRVDFNVPIDEDGVITDDKRI
                     LESLPTIKAITKNGGRCILMSHLGRPKGENNPKYSLSVVAEYLTSVLNFPVLFSDDCM
                     SENNIDVIDSMQNGDILLLENLRFYKEEEKNDPEFAKKLAAYGDIYVNDAFGTAHRAH
                     ASTEGVTHFIDRSAAGYLMEKELKYLSAAVSDPKRPLCAVLGGSKISGKIDVLENLLG
                     IADTILIGGGMMFTFYKAMGYNIGKSILEADKVELAKEIIEKAKTSDAKLLLPVDILM
                     AEKFEQGSKTIELPADGISSEYNDWTGVDIGPETIKTYSNEILNSKTIVWNGPMGVFE
                     IDDFAKGTMEVAKAMAEATSKGAFTIIGGGDSASAIAKAGLEKKITHVSTGGGASLEF
                     LEGKKLPGITALTDN"
     gene            complement(6803..7465)
                     /locus_tag="PLX80_11285"
     CDS             complement(6803..7465)
                     /locus_tag="PLX80_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21416.1"
                     /translation="MKHNFTTLISACLIIFSPSQSYSQLPYPTKYPTAVIPHNGDGGR
                     FPFLYMPFEYQDNSIQDTFRMYYVIRNPAGYKYYLARSIDYGITWSDTQRVIVPSEES
                     IVKVPDSTKLYMAYGTRIMQASFMNTSESTDGGFNFSNNSQTMELGEDKSFIWFEDTG
                     EYWGYVRPYNIEPSCCGFYDPPTCISYGDVFNGSDLASGVYFYKLSFGDFVQTKKLSL
                     IK"
BASE COUNT         2782 a         1357 c         1524 g         2335 t
ORIGIN      
        1 attatcagaa ttttgcaatc gtaggttatg tattaaaaga aggcggtcct cctcctccgg
       61 tttgtaatta tcagtggagt tcacaaacat caggatcgac aggtcagttc ttctcannnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaaa cggattctca gctacaaaga
      241 aaatgatgct gatcaagtaa aaagcaattt tacagaacaa ttagaaatca gcaattagaa
      301 attagaaatt agtaatcagg aattaagaat cagtaataaa tataaacgca atgttcatta
      361 agttggacat tgcgtttttt atttataaaa tcgattatct gttttcaaaa aaggaagtga
      421 tcaaatctaa aatacagatt cagtaattaa aaaatattgg atcaatcaaa taaatatttc
      481 aaaatatgaa ttataattta tttttttggc aataaaagta aattgacaat aaaattcatt
      541 ataaataaat taaaaacatt attaacacat ttttattaaa tttcaaaatt aaattattaa
      601 aaaaatgaaa tcaaaggttt acatttattc attattagtt gtaataattg cggctattta
      661 tggatttacc tcaatggatc caattactaa ggatcttacg caaagaaatt ccagttttaa
      721 caatccaatg tatacatacg gagctcagct tgaaaatcca ataagtacat taaatgagtc
      781 atttgaaagt gcaactttcc cgccaacagg atggacaaag atcaatcctg atgcaggaac
      841 aggctgggac agacagacag taggaacaac acctcttcca ggatggaatg gtggtgtaat
      901 aacttcacct cccggtggtg gtatcggagt tgcatttgct acctggacta caggtggaac
      961 tgttagtaat aatcagtggc ttgtaacttc acaattgact aattcaacag ttaatgattc
     1021 tttaagtttt tggaccagat tttggccaag taacatttat tctgatacag tagatataaa
     1081 aatttctaca actactccaa ctatcggtgg ttttacaaca aatgttgcta ctatagtttt
     1141 cccaagaaat tctccggata caaactggac acaacataaa tatgctatcg gaagtatgat
     1201 ccctgcggga tctaattatt acattgcttt cagagaaaga gtattagata acttaaatga
     1261 cggtgcatca gtatctctgg atctggtgtc agttactaca tcaacaggac ctccggcttg
     1321 taattatcag tggagttcac aaacatcagg atcgacaggt cagttcttct cagtatcagc
     1381 agtatcagat atggtctgct gggcagccgg tgcaacagct acagtaagaa agacaacaaa
     1441 cggcggatct acctgggaaa acggaaattc atcaccgggt gtaatagcag gtgatatcta
     1501 taatatctat gcatggagcg caaacgatgc aatttgtaca acttcacctg cagcatcaaa
     1561 catatataaa acaacaaacg gcggagccac atggacgtta tctcataccg ttgcaggcgg
     1621 atttattaat gcacttcaga tggtatcagc cacagaagga tatgcaacag gtgatcctgt
     1681 cggcggaatc tgggaattct taaagacaac tgacggcggc acaacatggg cacaggtacc
     1741 aacagctcct gcacaggtag gtactgaagc aggatggaac aactcatttt ctatcatagg
     1801 aacaaacata tggtggggca caaactcaac aaaagtttac agatcaacag atatgggtgc
     1861 aacatggtca agtggtgtat caaccggaac agtaaataca tatgcacttc attataacaa
     1921 tccgacaaca ggtatagcag gcggaacagc aactgtagta tcagcaaatg gcggaacatc
     1981 atatacagca gctacaagtc ccggaacttc aggaaacata aacggatttg aaggtgtcgg
     2041 aacagactgg tggggaataa gatcaggtgc aacagtttac agatccacaa acgcagctgc
     2101 aacatggaca gatgcttata cacaaaccgg agcagtttat caggatatag atttcgtaat
     2161 agttaacgga tgtcctgtag gctgggcagt tggtaactta ggtgtagttt caaaaatgag
     2221 caacgttaca ggaatctcaa acataggcgg agaagttcct gttgactata cattaggtca
     2281 gaactatccg aatccattca acccggagac aaacattaat ttcacaattc ctaaatcagg
     2341 aaacgtaaca ttaaagattt atgatgtctc aggaaaagaa gtttcaacat tagtaaatga
     2401 agtgaagaat gcaggaaact acatagtagg attcaatgca gcaagtcttc caagtggtgc
     2461 atacttctac agactggaaa caaacggatt ctcagctaca aagaaaatga tgctgatcaa
     2521 gtaaaaagca atttttcaga acaattagaa attagtaatt agaaattaga aattagtaat
     2581 tagaaatcag taataactat aaacgcaatg ttcattaagt tggacattgc gttttttatt
     2641 tatcagtttt gatcatcaaa ctaaattcct ttttcactga acttaattaa atttataaaa
     2701 aaaatactgt gcagtcattt caattccata ttgaataatt aaatgtaatt gtttaagtta
     2761 ggttaaattg aataaataat tttttataaa tcaaaccaaa ctaaaatgaa aaataaatta
     2821 catttttatt tcattccatt attactgata ctttttatgg tatcaggttt ttctgacaaa
     2881 gaaattcatc aaaacctgaa ggacacagaa tttaaaatcc agaataattc cggttcagac
     2941 acaataccac cggcaaattt cccttatcca gtagtatata actggtatta tcagaatata
     3001 cccggatcga attttgaaac agtaggtgca acatattttc aggataagta tgttttaaac
     3061 agatgggata attctacatt atacagatat aataaaaacg gacccggcgg cggtcccggt
     3121 tctttatcag attcgaataa ttcttatgca ggacagatca gagatatgac tgttgcgcct
     3181 gacggatccg ggcaaagttt tctctgggga ggaagagctt cgactgtact ttataaaatg
     3241 gatgccaacg gaaacaatat aaaacagttt tctcatacag gagctgcata cagagctatc
     3301 gcatgggatc caaacagaaa aggattctgg agttgtaatt tttccgacaa tatagtatgc
     3361 cgtgatacaa acggagttgt acttgctaca ttgccaaata ccctgaccgg aaaatacgga
     3421 atgggatttg acagtacatc atctgtagat tctgctttcc tttgggtctg gtctcaggga
     3481 ggaacaggtc tgccaaatac tcttcaaaaa attcatatac tatcaaattc cgtagctgct
     3541 gattatacat ttcagcttgc aaatggaaca gacggaattg ccggcggagc agaggttttt
     3601 ataaaagata atcaacttat attatctttg aattttcagg agtttgcaat agtaggatat
     3661 aaattaaaag atattggtcc gccgccgcct gtttgtaatt taatatggtt tccacagacc
     3721 tccggtactg taaacttatt atattcagta tcagcagttt ccgagcagat ctgctgggca
     3781 ggcggtgcag caggtacagt aagaaagaca acaaacggcg gagctacatg ggcagacgga
     3841 aattcaactc ccggaacaat tgcaggagat atatataata tttatgcctg gagtgcaaac
     3901 gatgcattat gtaccacttc acctgcagct tcaaacatat ataaaacaac aaacggcgga
     3961 accacatgga cacaggtctt cacacttccc ggcggattca tcaatgcaat tgaaatggta
     4021 tccccgacag aaggttatgc tgaaggcgat cctgtcggag gagtatggac aattttaaaa
     4081 acaactgacg caggatcaag ctggacacaa atgcccactg cacctgctca ggtcggcggc
     4141 gaggcaggat ggaataattc attcacagca agaggatcaa acatctggtt tggaaccaac
     4201 agtacaagag tttacagatc cacagatctg ggacttacat ggacaagcgg agcaacgaca
     4261 ggtacattga atacttattc acttcatttt aacaatccga atgccggcat ggcaggtgga
     4321 aatgcaatgg tgttatcaaa tgacggcggt gcaaattatt catcaatacc gagtcccgga
     4381 gtttcaggaa atataaattc aattgaaggt cacggatcaa actggtgggc tacaagatca
     4441 ggtgctgata tttacagatc ttccaacaac ggaatttcct ggaatactgc ttatacacag
     4501 gcagtaacca cattcaatga tatggatttt attgaagtgt cgggatgtcc atatggatgg
     4561 gcagttggtt catcaggagc cattgcaaaa atgaatttcc ctgtaggtat ttcaaatacc
     4621 ggcggtgaag ttccggttga ttatatatta ggtcaaaact atccgaaccc attcaatcct
     4681 gagactaata ttaaattcac aattcctaag tcagggatcg ttactttgaa aatttatgat
     4741 gtctcaggaa aagaagtatc tactttagta aatgaagtta agaatgcagg aaactatatc
     4801 gcaggtttca atgcaggtga tcttccaagc ggcgcttact tctacagact tgaatctgaa
     4861 ggattctctg cttcaaaaaa aatgatgctg ataaaataaa gagcagttaa aaatcaggaa
     4921 tcaggaatta ataatttatt taacgcaatg ttcatttgac tgaacattgc gttttttttt
     4981 agggaaattt aaaacatcac atccgtaatt tcaccacaaa gacactaagt cacaaagata
     5041 aatatttatt aactttttct tttgtgtctt tgtgtctttg tgtcttggtg tcttggtggt
     5101 aagaacagat gtttagggat agttttcatt taaaattaat aaatagtatt tctcaataat
     5161 tttagaacta tttatttaaa atttaaaata ttccggacag caccggtcaa acataatgac
     5221 attatttaaa aaggtctgta attccttatt taaaaatatt atttttacaa attccaaaaa
     5281 ttaaccaatc taaaataatg tcagataaaa aactttcatt gcagaaactt attgataata
     5341 atgagatagc cggtaaaagg gttttggtaa gagttgattt taatgtccca atagatgaag
     5401 acggtgtgat cactgatgat aaaagaattc tggaatctct tccgactata aaagcaatta
     5461 caaaaaacgg cggcagatgt attctgatga gtcatctcgg cagacctaaa ggtgaaaata
     5521 atccaaagta ttctctttct gtagttgctg aatatttaac cagtgttttg aattttcctg
     5581 tactgttttc tgatgattgt atgagtgaaa ataatattga tgttatagat tcaatgcaga
     5641 acggagatat actgctgctt gagaatctta gattttataa agaggaagaa aagaatgatc
     5701 ctgaatttgc aaaaaaactt gcagcttacg gagatattta tgtgaacgat gcattcggaa
     5761 ctgctcacag agctcatgct tctactgaag gtgtcactca ttttatagac agatctgctg
     5821 cgggatatct gatggaaaaa gaactgaaat atttatctgc tgctgtcagc gatccgaaga
     5881 gaccgctgtg tgcagtactc ggcggctcga agatctcggg aaaaattgat gtgcttgaaa
     5941 atttacttgg cattgctgat acaattctga tcggcggggg aatgatgttt actttttata
     6001 aagcgatggg atataatatc gggaagtcta ttcttgaagc agataaagta gagctggcaa
     6061 aagagatcat tgaaaaagct aaaacatcag atgctaaact attattgcct gtagatattc
     6121 tcatggcgga aaaatttgaa cagggatcga aaacgataga actgcctgct gacgggatct
     6181 cttccgaata caatgactgg accggtgtcg atattggtcc tgaaaccatt aagacatatt
     6241 cgaatgaaat tctgaattca aaaacaatag tgtggaatgg tccgatggga gtttttgaaa
     6301 ttgatgattt cgcaaaaggt acaatggagg tagcgaaggc aatggcagaa gcaacatcaa
     6361 aaggcgcatt tacaattatc ggcggaggtg attcagcaag cgcaattgca aaagccggac
     6421 ttgaaaaaaa gatcacacat gtttctaccg gcggcggagc ttctcttgaa tttctggaag
     6481 ggaaaaaact gcccggaata acagcgctaa ctgataattg agaattacaa atttcaaatt
     6541 acaaataata attttaagag ttactggtta cgaattacga gttgtgaatt atgagttacg
     6601 tgatacgaga tacgaattac aagaattact tttttgcaac ttaaattaat cacataatgc
     6661 taaggatata tacaataaag attattgaga aataagttta aatttataat agcacttttc
     6721 agaatacttc ataagtaatt cccccttaaa aaagcgggag cgaagggggt tgttgccaaa
     6781 acttttttaa acagagaaat tctcatttta taagtgataa ctttttcgtc tgtacaaaat
     6841 ctccaaaact taatttataa aaataaacac cacttgcaag atcacttccg ttgaatacat
     6901 caccatatga tatacaagtt ggtggatcat aaaatccaca acaagaaggt tcaatattgt
     6961 aaggtctcac atatccccaa tattcccctg tgtcttcaaa ccaaataaat gatttatctt
     7021 cacctaattc cattgtttgt gaattattag aaaaattgaa tcctccgtca gttgattcag
     7081 atgtattcat aaatgatgct tgcattatcc gggtaccata tgccatatat agttttgtcg
     7141 aatcaggaac tttaacaatg gattcttctg aagggactat cactcgttga gtatctgacc
     7201 aggttattcc ataatcaatt gatcttgcta aataatattt atatcctgcc ggatttctaa
     7261 tcacataata cattctaaaa gtatcttgta tggaattatc ctgatattca aatggcatat
     7321 ataaaaaagg aaatcttcct ccatctccat tatgaggaat tactgccgtt gggtattttg
     7381 tcggataagg aagttgactg taagattgag aaggactgaa gattattaag caagcactta
     7441 ttagagtagt aaaattgtgt ttcatattta ctccttttta aaattcttta atatgtaacc
     7501 tatagaattt ttaaaatcaa gacacggaag tatacataga aatttagata acgttccatt
     7561 tagtgtgtaa aaactaaatt gctattcgta ggaataaagc ctctcctttg atttaaatat
     7621 tcaacggtta agaataataa tttatcagca ctctttaccg tctcaaaaat tttgtgccat
     7681 tgttggtgtt ctaaaaaggt tgctcagtaa atcttaaatt tatcagccca actcaagcaa
     7741 gctttttggc caccaacaac agtgccggtg aagtctgctg taattaagat tgttaaaccg
     7801 aatttaaatt aaatccgcag atgatatttt acaagtgatt tatttcatta catcatagag
     7861 aggtgtgcag cagagtctgt tgttggtgat caaaaagttt gattaagatc tatagtaata
     7921 ttcaactgaa cctcaggcaa actttttgaa aacaccaaca acggcgggaa aaaagattgc
     7981 tttgtgtctt tgtggtatta attaaaatta tgttttgaga cactttcgga cgaggctgtt
     8041 tcaaaacact atttttacca ccaagacacc aagacacaaa gaaaaaactt taaaaaaa
//
LOCUS       DAORVQ010000157         8002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5198_length_8002_cov_5.331446, whole genome shotgun sequence.
ACCESSION   DAORVQ010000157 DAORVQ010000000
VERSION     DAORVQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_8002_cov_5.331446"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLX80_11290"
     CDS             <1..407
                     /locus_tag="PLX80_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQY21417.1"
                     /translation="IGKLYLSPSQLIKSQLKADELLIEDLKEHIITLESSGQRVDREK
                     VDYYSKISFPFSNLIVILFGISISTNRRKSGTAVQFGISILVTFIYLGFLKVSQTFGY
                     NGDLNPLLTAWLANILFLLLAIVNLLRMNYIK"
     gene            complement(417..764)
                     /locus_tag="PLX80_11295"
     CDS             complement(417..764)
                     /locus_tag="PLX80_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQY21418.1"
                     /translation="MDNKNNKDPQNQSQQINIELGEKEAEGIYSNLAIISHSPAEFII
                     DFTRIFPGIPKAKVHSRIVMTPQHTKLFFNALKENMERYENDFGEIVVHQGVNNQGMG
                     IQLPVPPKDEKIN"
     gene            970..1890
                     /locus_tag="PLX80_11300"
     CDS             970..1890
                     /locus_tag="PLX80_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQY21419.1"
                     /translation="MSLLVIGSLALDTIETPFGKAERTLGGSATFISTSASYFTTPVR
                     LVGIVGYDFPKEEIEFLKSKKIDISGLEISETKKTFHWHGKYDFDLNTRESLVTELNA
                     FETFDPVISEKFKDSEYVCLGNLHPSLQKKVISQISKPKLVMCDTMNFWIEGAYNELL
                     EILKHVNLLVINDSEARELTKEFNLVIAARKIMSLGPKILIIKKGEHGALLFTNDSIF
                     SAPAYPLEEVFDPTGAGDTFAGGLMGWIAKTKDLSTDNLKLAVIYGSTMASLAVEKFS
                     LDGIRNLTNDTIMKRFSEFKKLTFFKEQTL"
     gene            1929..2630
                     /gene="recO"
                     /locus_tag="PLX80_11305"
     CDS             1929..2630
                     /gene="recO"
                     /locus_tag="PLX80_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQY21420.1"
                     /translation="MSIIKTDVIVLKSIKYGDTSKIVTLYSKDLGKVKAIIKGARNYK
                     SGMSGIFQTLNFLSVIIYFKESREIQNLSKAEFITSFKNITEDFDKLQIALRVIEIIN
                     KSLPDMDPNPELFKIASVFFTGINNPDSDNKLLLLNFQIEFLKISGMYPDLKDVQKNF
                     ETLKNNTEFNLNDEDINFLLKLSDSKTSEETISNERSNYNVSKISDCFSSYIVNHNST
                     KIFFKTDKVFREINK"
     gene            2714..4222
                     /locus_tag="PLX80_11310"
     CDS             2714..4222
                     /locus_tag="PLX80_11310"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HQY21421.1"
                     /translation="MSKKSIVFAGLLLVIALSFGGLLIANFGDVKQIIASSQIEYKTT
                     PPITNVSPEIKNLNEAFVQIAKSVTPAVAYIEVTTGSSESDEQNPGFNMPFDLGPDFQ
                     MPDQGPSRGSGSGIIISNDGYILTNNHVIKNATENGIKVRLTDKREFNAKLIGVDPNT
                     DVAVIKIEASDLMVASIGNSDDVQVGQWVVAIGNPLGLNSTVTAGIVSALGRNIQMGG
                     DSYAINNFIQTDAAINPGNSGGALVDINGSVIGINTAIKTTNGYYQGYGFAIPINLAQ
                     IVATELIKSGKISRGYIGVSIKDVDIKEAKGLGLDKARGVLVQDVLAGGAGDEAGLKV
                     GDVILFVDNQEVNASNELQTIIGTHRPGDVVDLKVFRDGSEINKKVTLKPRSETNNQT
                     AQNTTPKEETGDLNTKSFESIGLTVSELNSQLKSKFSVESGVYINSVKQFSEAFDRGL
                     REGLVIIEADKNEINSVSELNSIIETKSTGDIIVFKVKTSDGLVRLIAVEIE"
     gene            4340..4732
                     /locus_tag="PLX80_11315"
     CDS             4340..4732
                     /locus_tag="PLX80_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21422.1"
                     /translation="MIKIIKIFVIILTVGFSLNVYANSNNSADNSVNSGPKNTFGVSD
                     DYKLYQNFPNPFNPATIINFKINQSGFVTLNVYNLVGQVVKTLVNDYRQTGTYSEQFD
                     ASELSAGVYLYKLQVNNFTSVKRMTLIK"
     gene            4768..5667
                     /locus_tag="PLX80_11320"
     CDS             4768..5667
                     /locus_tag="PLX80_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21423.1"
                     /translation="MTNIKIIFTLTFLVILFNNSFSQGIIIDDFSQKALPGWIWGGID
                     MKYSHEEDNKENGFAELFSKGEIKADSYIGKIFLQRDHTFTVGNFVNAMFKGVNNDAY
                     VKIQLIYDVDNNSTYNEDEDLMLVTINPVSMDFDGWKEIKVKLDQDNFKIISKYKDNF
                     EVTEDKILGIQFEFLSGKNYKDSKFESGIALISEIPNKETASDYEGSISTNKESYFNA
                     TNDPNPFKSSTTIKYNLPNATFINITVYDRLGKEVINLVNQDQSSGEHTVEFNSDDYP
                     SGIYFYRIKTTEKTEVRKMLLAR"
     gene            complement(5679..7145)
                     /locus_tag="PLX80_11325"
     CDS             complement(5679..7145)
                     /locus_tag="PLX80_11325"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQY21424.1"
                     /translation="MFSLIIIVVSINSCEQDFVSNNPDIVEFPPEIKAIFDTPFTQTN
                     LSCSTPSCHGNDNSQSGGLNLVDWQKAMNGSDNGTMIVPYNGFWSHLYSVVNSDTLVG
                     PVSLVSFSDQHKISAQNVATLKNWMDEGAKSEDGQVAFTSIPKEEKCLITNQASDLVA
                     VLQADYKKIIRLIPVGGSNTLDAPHYVKISPDKNFFYISLIQEGYVEKYDINIDFPFT
                     KSDRLQVGTSPAHIEISPDGLTGYVTNFVASGAGTIRKFSTNSPMQVTGILEDVKLTA
                     PHGMALTADGNVLFVASEIGEYLFRINTNSFVNSDSTVIKEPIDPSVPPNGQGTGNFK
                     PYQIILSQDESLLYVSCRGSNEVRIYRASDLVMVNQIFLGTNAYPLLMNLTSDGKYLF
                     TCNRNNNTVSVINTITQSIHTTVTNVGIQPHGVDFTPDGQYAIIACETLSGFDGHHPT
                     VGTNKVGVSRLIRMSDFTLTEDRLEMASFPAGIETVNK"
     gene            complement(7177..7626)
                     /locus_tag="PLX80_11330"
     CDS             complement(7177..7626)
                     /locus_tag="PLX80_11330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQY21425.1"
                     /translation="MKIQDFKHITKVRVRTYDVDSQGIVHNMNYLKYYETGRVEYRRS
                     FGYKINRTGIFDDGLKVVVVRNEVDYISFAFLDDLLEIYTRISWVKNSSFCFEQLITK
                     ENDSQIISKAKGILVNINEKNVPETLSLKFLNEIKNFEKNFKLIKND"
     gene            7723..>8002
                     /locus_tag="PLX80_11335"
     CDS             7723..>8002
                     /locus_tag="PLX80_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HQY21426.1"
                     /translation="MINQKKIVNKKIGLIGFPMDLGADRRGVDMGPSAIRYENLEQKL
                     EKFGYKVTDFGDIEISISETQKIKNTSLKYLPEITKTSKFLANKIENLL"
BASE COUNT         2893 a         1176 c         1280 g         2653 t
ORIGIN      
        1 aaattggaaa actgtacctc tccccttctc agttaattaa aagtcagtta aaagcagatg
       61 aacttctgat tgaagatctc aaggaacata taattacttt ggaatccagc ggacagaggg
      121 ttgaccgtga aaaggtcgac tattactcaa agatatcttt ccctttttca aatttgatag
      181 tgatattatt tggaatttct atcagtacaa acagaaggaa aagcggtact gcagttcaat
      241 ttggaataag tatattagtt acatttattt atctgggatt tttaaaagta tcacagacat
      301 ttggctacaa cggagacttg aatcccctgc tcacagcctg gcttgcaaat atattatttc
      361 ttttgctggc gattgtaaat ttattaagaa tgaattacat aaagtaaaac ataaagttaa
      421 tttatttttt catcttttgg cggaaccggc aactggattc ccattccctg attatttaca
      481 ccttgatgta ctacaatttc accaaaatca ttttcatatc tttccatatt ttctttcaat
      541 gcattaaaaa ataattttgt atgctgtgga gtcataacta ttctagaatg gacttttgct
      601 tttggtatac ccggaaaaat tctagtaaaa tcaattataa attcagcagg tgaatgtgag
      661 atgatagcca gatttgaata aataccttct gcttcttttt ctcctaattc tatatttatt
      721 tgctgagact ggttttgtgg atctttgtta tttttattat ccattatatt gttgaaattt
      781 ttatttttgt gaataaaatc agttaataaa atatactttt attttataaa attcaaacta
      841 atataattta agccgggtaa agcaatcatt tattttttat gattcgaaga taaattttaa
      901 gcaatattat cttcttgaat aagggattca ttataattat tttgcagttt ataataaaat
      961 atttaaataa tgagtttact agtaattggt tcgttagcat tagacacaat tgaaactcct
     1021 tttggtaaag cagaaagaac acttggcggt tcagcaactt ttatatcgac ctcagcaagt
     1081 tattttacaa caccggtaag acttgtcgga atagtaggat atgattttcc aaaagaggaa
     1141 attgaatttt tgaaatcaaa aaaaattgat atttcaggct tggaaatatc tgaaacaaaa
     1201 aagacttttc actggcacgg taaatatgat tttgatctaa ataccagaga atcactagta
     1261 actgaattga atgcatttga aacattcgat cctgtcattt cagagaaatt taaagacagc
     1321 gaatatgtat gtcttggaaa tctgcatcca tcacttcaga aaaaagttat aagtcagata
     1381 agtaagccga aacttgttat gtgtgacact atgaatttct ggattgaagg agcttataat
     1441 gaattactcg aaatacttaa acatgttaat ctgcttgtta tcaatgacag tgaggcaaga
     1501 gaacttacaa aagaatttaa cttagtaatt gctgctagaa aaattatgag cctgggtccg
     1561 aaaattctga taataaaaaa aggcgaacac ggagctttgc tatttacaaa tgattctata
     1621 ttttcagcac ctgcataccc tcttgaagaa gtatttgatc cgaccggtgc aggtgataca
     1681 tttgcaggag gactgatggg atggattgcg aaaacaaaag atttaagtac agataatctt
     1741 aaacttgcag taatttacgg aagtactatg gcttctcttg cggttgaaaa attttcactt
     1801 gatggtatca gaaatttaac aaatgacaca ataatgaaaa gattttctga gtttaaaaaa
     1861 ttaacttttt ttaaagaaca gactttataa tactttgaaa caccttttta aaaattaacg
     1921 taacagccgt gtctatcata aaaactgatg taattgtatt aaagtctata aaatatggtg
     1981 acacgagtaa aattgtaaca ctttattcaa aagatctggg aaaggtaaaa gcaataataa
     2041 aaggtgccag aaactataaa tcaggtatgt cagggatttt tcaaactctg aattttttat
     2101 cagtaatcat atattttaaa gagagcagag agattcagaa tttatctaaa gcagaattta
     2161 ttacttcctt taagaatatt acggaggact ttgataaact acagatagct ttaagggtaa
     2221 ttgaaattat taataaatct ttgcctgata tggatcctaa ccctgaatta tttaaaattg
     2281 catcagtatt ttttacagga attaataatc ctgattctga taataaactt ttattactta
     2341 atttccagat tgaattcttg aaaatttccg gaatgtatcc tgatttaaaa gatgtacaga
     2401 aaaattttga aactttaaaa aacaatactg aatttaactt gaacgatgaa gatataaatt
     2461 ttcttttaaa actttcagat tcaaaaacaa gcgaagaaac tatttccaat gaaagaagta
     2521 attataatgt atcaaaaatt tcagattgtt ttagcagtta tattgtaaat cacaattcaa
     2581 caaaaatatt ttttaaaaca gataaagtat ttagagaaat aaataagtaa attaaaagga
     2641 acaatattta ttttaacgaa taaaaatgta aagttattaa aacctaattt taaaaactaa
     2701 ggagataaaa ataatgtcaa aaaaatcaat tgtttttgca ggtctattat tagtaatagc
     2761 tctttcattc ggaggtttgc tgatagcaaa tttcggagat gtaaagcaga tcattgcaag
     2821 ctcgcagata gaatataaaa ccactccacc tatcactaat gtaagccctg agatcaaaaa
     2881 cttaaatgaa gcatttgttc aaatagcaaa atcggtaact cctgcagttg cctatattga
     2941 agttacaact ggaagctcag aaagtgatga acaaaatcct ggattcaata tgccgtttga
     3001 tttaggtccc gattttcaaa tgcctgatca aggaccatcc agaggaagtg gttcaggaat
     3061 aatcattagc aatgacggtt atattcttac aaataatcat gtaattaaaa atgcaacaga
     3121 aaatggtatt aaagtcagat taactgataa aagagaattt aatgctaaat taatcggggt
     3181 tgatccaaat acagatgttg ctgtaataaa aatcgaagcc agcgatctaa tggttgcctc
     3241 aattggtaat tctgacgatg ttcaggttgg acaatgggtt gtcgcaattg gaaatccttt
     3301 aggattaaat agtactgtaa ctgccggtat tgtttcagct ttaggaagaa acatacaaat
     3361 gggtggagac agctacgcaa tcaataattt tattcaaact gatgcagcaa ttaatccggg
     3421 aaatagtggc ggtgctttag ttgatatcaa tggttctgta attggaatta atacggctat
     3481 aaaaacaaca aatggttatt atcagggata tggatttgca attccaataa acttagcaca
     3541 aatagttgca actgaactta taaaaagcgg taagatctca agaggataca ttggagtttc
     3601 aataaaagat gttgatatta aagaagctaa aggtcttgga ttagataaag ctagaggagt
     3661 attggtacag gatgttcttg caggcggagc cggagatgaa gcaggtttaa aagtcggaga
     3721 tgtaatcctg tttgttgaca atcaagaagt taatgcttcg aatgagttac aaactataat
     3781 cggtacgcac agacctggag atgtagtaga tctaaaagta ttcagagatg gaagcgaaat
     3841 taataaaaaa gtaactttaa aaccaagatc agaaacaaac aatcaaactg ctcaaaatac
     3901 tacaccaaaa gaagaaaccg gagatttaaa tacaaaaagt tttgaatcta ttggattgac
     3961 tgtttcagaa ttgaacagtc agcttaaaag taaattttca gttgaaagtg gtgtttatat
     4021 aaattcagtt aagcaatttt ctgaagcttt tgacagagga ctaagagaag gtcttgtcat
     4081 tattgaagca gataaaaatg aaataaacag tgtgagtgaa cttaatagta taatagaaac
     4141 taaatccaca ggagatataa ttgtttttaa agttaaaact tccgatggtt tggttagact
     4201 tatagctgtt gaaattgaat aatttgttaa gttccggaat gtcaagtaac cattccggaa
     4261 ctatttttat aaatcttagt ttgactatat tgtgttattg attattaaat atattttcag
     4321 taaatttgta ttaagagata tgataaaaat aataaaaatt ttcgtaataa tattaactgt
     4381 tggatttagt ctaaatgtct atgctaactc aaacaacagt gctgataaca gtgttaattc
     4441 cggacctaaa aatactttcg gagtttcaga tgattataag ctttatcaaa actttccaaa
     4501 tcctttcaat cctgcaacaa taataaactt caaaattaac caaagtggat ttgttacatt
     4561 aaatgtatat aatctggttg gtcaggttgt aaaaactctt gtaaatgatt atcgacaaac
     4621 cggaacttac agtgagcagt ttgatgcttc tgaactttca gccggtgtat atctttataa
     4681 attacaggta aataatttta cttccgttaa aagaatgaca ctgattaagt aatactaaaa
     4741 tcagttcgtc ttcaaattaa atcatacatg acaaatatta agataatatt tactttaaca
     4801 tttcttgtaa ttttatttaa taattcattt tcacaaggaa ttataattga tgatttttca
     4861 caaaaagccc ttccaggctg gatatggggt ggtattgaca tgaagtattc tcatgaagaa
     4921 gataataagg aaaatggttt tgctgaattg ttctcaaaag gagaaataaa agctgatagt
     4981 tatattggaa agatctttct gcaaagagat catactttta ctgttgggaa ttttgtgaat
     5041 gctatgttta aaggtgttaa taatgatgca tatgtgaaaa ttcaattgat ctatgatgta
     5101 gataataaca gtacttataa cgaagatgaa gatctaatgc ttgtaacaat aaatccagtt
     5161 tctatggatt ttgatggttg gaaagaaata aaggttaaat tagatcagga taatttcaaa
     5221 ataatctcaa aatataaaga caattttgaa gttactgaag acaaaatttt aggaatacaa
     5281 tttgaatttt tatccggaaa aaattataaa gactcaaaat ttgaatcagg aatagcatta
     5341 atctcagaaa ttcctaataa agaaaccgca tctgactatg aaggatctat ttctaccaat
     5401 aaagaatctt acttcaatgc tacaaatgat ccaaaccctt ttaaatcatc aacaacaata
     5461 aaatataatc ttccaaacgc aacttttata aatatcacag tttatgatag attaggtaag
     5521 gaagtaataa accttgtaaa tcaggatcaa tcttctgggg aacatacagt tgaattcaat
     5581 tccgatgatt atccaagtgg aatatatttt taccgtataa aaactaccga aaaaacagaa
     5641 gtcagaaaga tgttactcgc cagataattc tctttatttt atttatttac agtttcaata
     5701 ccggcaggaa atgaagccat ttccagcctg tcttccgtta aagtaaaatc tgacattcta
     5761 attaatcttg aaactccaac tttattagtt ccgactgttg ggtgatgacc gtcaaatcct
     5821 gataatgttt cacatgctat tattgcatac tgaccatccg gagtaaaatc aacaccatgt
     5881 ggctggatac caacatttgt aactgttgta tggattgatt gtgtaattgt atttatcact
     5941 gatactgtgt tattatttct gttacatgtg aaaagatatt taccatcaga tgttaagttc
     6001 attaatagag gatatgcgtt tgtaccaagg aagatctgat ttaccattac aaggtcactt
     6061 gctctgtaaa ttcttacttc attagaacct ctgcatgata catataataa agattcatcc
     6121 tgggaaagta ttatctgata gggcttgaaa tttccggtac cttgtccgtt aggtggtaca
     6181 cttgggtcaa ttggttcttt aataactgtc gaatctgaat ttacaaatga atttgtatta
     6241 attctgaaca gatactcccc tatttctgat gcaacaaaaa gtacatttcc atctgcagtt
     6301 aaagccattc catgaggtgc agtcaatttt acatcttcca aaattccagt tacctgcatt
     6361 ggtgaatttg tactgaattt tctgattgta ccggcacctg aagctacaaa atttgtaaca
     6421 tatcctgtca gaccgtctgg tgaaatctct atatgtgccg gacttgtacc aacctgtaat
     6481 ctgtcagact ttgtaaaagg aaaatcaata ttaatgtcat atttttcaac ataaccctcc
     6541 tggatcaatg agatataaaa gaaattttta tcgggactaa ttttcacata gtgtggtgca
     6601 tccaaagtat ttgaaccacc gacaggaatt aatcgaataa tctttttata atcagcctgt
     6661 aaaactgcta caagatcaga agcctgattt gtaatcagac acttttcttc ttttggaatc
     6721 gatgtaaatg ctacttgccc atcttcactc ttggcacctt catccatcca gttttttaat
     6781 gtcgctacat tctgtgcact tattttatgc tgatctgaaa aactaactaa actaacagga
     6841 cctactaaag tgtcactatt cacaacagaa taaagatgtg accaaaatcc attgtaagga
     6901 actatcatag taccattatc agatccgttc attgcttttt gccagtccac caaattcaaa
     6961 ccaccgcttt gagaattatc atttccatga caagaaggag tactgcagga caaatttgtc
     7021 tgagtgaatg gagtatcaaa tatcgctttt atttcagggg gaaactcaac aatatcagga
     7081 ttattactta caaaatcctg ttcacaagaa tttattgaaa ctacaataat tatcagtgaa
     7141 aacaaagaaa tgcaattcaa taaattttta actcggttaa tcatttttaa ttaatttaaa
     7201 atttttttca aagtttttaa tttcatttag aaatttcaag cttaatgttt caggtacatt
     7261 cttttcgttt atattaacta atataccttt tgcttttgaa attatttggg aatcattttc
     7321 ttttgtgatt aattgttcaa aacaaaaact ggagtttttt acccatgata tgcgcgtgta
     7381 tatttctaat aaatcatcaa gaaaagcaaa cgaaatataa tctacttcgt tccttactac
     7441 gactactttt aaaccatcat caaatatgcc ggttctgtta attttatatc caaaacttct
     7501 tctatattcc actcttcctg tttcataata tttcaaataa ttcatattat gaacaatacc
     7561 ctgtgaatcc acatcataag ttcttactct taccttggtt atatgcttaa agtcctgaat
     7621 tttcaattat gcagataaat taaaaaattt aattcagaat taaaattcaa atgtaatttt
     7681 aaacttactt aaatatttgc attctaatca ctatatttgt ccatgataaa tcaaaaaaag
     7741 atagtaaata aaaagatcgg tctgatcggt tttccaatgg atctaggagc agacagaaga
     7801 ggagtcgata tgggaccttc tgcaattcgt tatgaaaatc ttgaacaaaa acttgagaaa
     7861 ttcggatata aagttactga ttttggcgac atcgaaatta gtatttccga aacacagaaa
     7921 ataaaaaata caagtttaaa gtatctacct gaaatcacga aaacatccaa gttccttgca
     7981 aataagattg agaatctttt ag
//
LOCUS       DAORVQ010000158         7956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5241_length_7956_cov_5.650930, whole genome shotgun sequence.
ACCESSION   DAORVQ010000158 DAORVQ010000000
VERSION     DAORVQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5241_length_7956_cov_5.650930"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(406..750)
                     /locus_tag="PLX80_11340"
     CDS             complement(406..750)
                     /locus_tag="PLX80_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21427.1"
                     /translation="MENDLKKETVKKAAKKQAVKKAAKKNVLKGAVKKKAIKKAVKKD
                     AAKKKAVKNAVKKNAVKGAVKKKAVKKKVIKDAVKKNTLKKAAVKQAIKNRLVKKAIV
                     KAIMKEAISKKK"
     gene            complement(902..1921)
                     /locus_tag="PLX80_11345"
     CDS             complement(902..1921)
                     /locus_tag="PLX80_11345"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HQY21428.1"
                     /translation="MNAKILLSIFIFSLVSLVAITFTPADAEAQRMRGGSRGGRSGYS
                     GNRSGRTINGGNRNFNTNRNTTTRKNNTNRNKVNTNNKKINRDNKKRDNTNRNSRESI
                     RTNDTKDNKRDQSNRNDKERNDRNDRQDNRGDRQDDRQDKRGDRQDDRQDTRGDRRDN
                     FYDNNYNNYYHGYHYGGYYPYSYHSYYPFGWGPYWYPVGFFMLAMTTTAIVVSANDKE
                     EYYYDEGVYYDKVDSGYKVVQAPQGVVITSLPSGFTPAVVNGVTFYYHGGAFYQDTEG
                     GYKVVSAPAGAIIYNLPDGAEGVEINGNKILKYGGAFYQPISHEGSDAYEVVDINSSD
                     EKKKE"
     gene            complement(1929..2657)
                     /locus_tag="PLX80_11350"
     CDS             complement(1929..2657)
                     /locus_tag="PLX80_11350"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HQY21429.1"
                     /translation="MKLAILLFLVVSFCSSAFPEKSYSQFDSLAISILDSMSDRISEL
                     ETCSFKYYSEFDKNSEEYGMVTHSEFGKVFLKGPDKLFIEKKGDKGHNKIFYNGNLFL
                     LYSIDKNRYASMPTTMTLIEFIDSVSSTYGVEFPGADVFYPDFVDHILENSDNLICLG
                     LTMVGEKESYHIAGTKEDITFQIWVSSDGKFQPLKMMIDYILKPDTPRYRIVFADWIQ
                     DEILNDDMFEFTPPIDAEKIKLIK"
     gene            complement(2831..3865)
                     /gene="glsA"
                     /locus_tag="PLX80_11355"
     CDS             complement(2831..3865)
                     /gene="glsA"
                     /locus_tag="PLX80_11355"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HQY21430.1"
                     /translation="MNYQKENLVVKLLLVVIFSTFLISGQLYAQKLTKETIESAVKEA
                     YEKFKNVTDGKNADYIPALAEVDPNIFGISVVTVDGDVIEFGDIDDMVSMQSIEKVFA
                     MAMVIEDLGFEAIRDKVGVDATGMKFNAIEPIEWKKGNEMNPCVNPGALATTSLIKGT
                     TEEEKWNNIISMYSDFAGRTLTVNQPVYESEAATNQRNQALAWLLLAYERMYFDPVKT
                     TDIYTKACAINVNAKDLGMMAATLSNGGINPVTGKTVISSKTVEHVLPVMATAGLYDD
                     SGIWLYETGMPGKSGVGGGIIAVAPGVLGIGVISPPLDDVGNSVKAQLVIKFLSEKLG
                     LNMYSIQPSK"
     gene            complement(4243..5460)
                     /locus_tag="PLX80_11360"
     CDS             complement(4243..5460)
                     /locus_tag="PLX80_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HQY21431.1"
                     /translation="MKTKLLSRFVTAVVLLMIFCFSNFNIKAQNDKSFEVYGFTMAEA
                     GYNFKTINPDYYDALRVTRLPSFKGEFAPDGKLFYSVRQSRFGVKSTVPTGLGDLKTV
                     FEFDLYGVGADAGQTTIRPRQIYGQLGKFLAGQTNSTFMDIDVFPNSLEYWGPTGMIF
                     YRNIQLRYTPIMTKQDNLAMALEQPGASGDGGIYSNRVELTNVKPLFNVPDFTAHYRY
                     TGNFGYIQLGGIVGSIKWRDVSDTARFQLNGSAVRWGGTLSSNINIGKKAIFKIQGVY
                     GEGMQNYMNDAPVDIGIQSNPGDSLQPFKGKALPVWGITAFMDVNWSKLFSSSFGYSV
                     ETIDNSDLQTPNAYKQGQYGLVNLLYYPVENVMAGVEYNFGRRDNFSDGFHSTTSEVR
                     FAFKYNFSQIWKF"
     gene            5673..7103
                     /locus_tag="PLX80_11365"
     CDS             5673..7103
                     /locus_tag="PLX80_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21432.1"
                     /translation="MKIKKTLKFTTLYNSLTDSEKFEFYEYLKSSLSPKLHIPKSIIE
                     NFKPGVNISELQLNNLRSQTIWNINSQLSKALKMYIAIKELSNDPDLNYRLIRVQLKK
                     RNLDRLLTNKCVTRINELNKSKIQGNSFRNLADAYMDYLQTLLSDDDKKKQLEVYELY
                     NKYFSLSMIFELIILRFNNEVTSNSQKESKIYFLNDFYNCIDFGKIMELLKTNFPNYY
                     PVIGMWNDFYELYSVKFDLEKYHIVKNNFFSIMDQFSDELKSGSFNVLTDLLIVKREE
                     LGIDLDGELFEIFFKKEAQGLTEDFTFNRIGYNHFRDNILIAINVGEIEWAEYFLNKY
                     SDTLPEKQKNNDLNTGKARISFSKGEFKDSITFIGKLKKSSFIHYSDYFRISIKANFE
                     LSNYNECFLLAEKFSEYLKRNKNLPSSFVNGSKFFIKNIAVLSEYKMTSDRKRLKKID
                     MPKLQVKNSSNKWLLDKFNILIKSNS"
     gene            7305..>7956
                     /locus_tag="PLX80_11370"
     CDS             7305..>7956
                     /locus_tag="PLX80_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21433.1"
                     /translation="MFIDKIPLEVIFLATLIIFFISMEFGYRIGNRLSESAKKVKEKI
                     TSANASAVLTMLGFMLVFSFGIVYSRFDSKKELVLDEANLIRTAWLRSDFLQDQDRVE
                     TEKLLINYVDIRINAANEKDLSLIQNLLDQSNEIQMKLWNIAVANARIDMNSDIGALY
                     IEAVNEMINLQSKRVVLGIQARLPLAIWIMLFSLVIFGMICVGYETAINSSSKISWL"
BASE COUNT         2555 a         1387 c         1145 g         2869 t
ORIGIN      
        1 acttattatt tctaatgaaa aatatcattc tttctttaaa tattcattcc gtgagctagg
       61 ttttcctgct tattgagatt atttttaact ccattttatg tccggaaaat gctaatgaac
      121 actttagcat caggagcaaa tttaattcta taagaagcaa aatgagaaat tctctattaa
      181 aagcatcgta acttttacat ttaatagtct tttgtgtttt caaaggatta caataaaatg
      241 caggtaattc agaaatttga agttcatttt tacacctcaa ataatataaa aatattatcc
      301 atataaaaaa tcacttttaa aaaattcatt actaaaacag gagttgcagg gaaaatgtca
      361 caaaccctgg caactcctaa aatatattaa attgcgataa ctgatttact tctttttaga
      421 aatagcttct ttcataatag ctttaacaat tgcttttttt acaagacggt ttttaattgc
      481 ttgttttact gcagcttttt ttaatgtatt cttctttact gcgtctttta tcactttctt
      541 tttaactgcc ttctttttga cagcaccttt tacagcattt ttctttacag catttttaac
      601 tgctttcttt tttgctgcat cctttttaac agcttttttt attgcctttt ttttgacagc
      661 accttttaaa acattctttt tggctgcttt ttttaccgcc tgtttttttg ctgctttttt
      721 tacagtttct ttttttaaat cattttccat ttttttctcc aaattaattt aataaatagt
      781 ttatgcttaa ttgttacaaa tgaataatta cattttaaaa tttactgttc tcaaaaaatc
      841 aaatttcaaa aaaatgaatt atgatctttc aactaagttg taaagcaacg atccggagat
      901 attactcttt ctttttctca tcagaagaat ttatgtctac tacttcgtat gcatcacttc
      961 cttcatgtga aattggctga taaaaagctc cgccatattt aaggatttta tttccattta
     1021 tctcaacacc ttcagcacca tcaggaaggt tatatataat tgctcctgca ggagcagata
     1081 ctactttata gccgccttca gtatcctgat aaaatgcacc tccgtgataa tagaacgtta
     1141 caccgtttac aactgccgga gtaaaacccg atggcaaaga agtgataaca acaccctgcg
     1201 gggcctgtac cactttatag ccgctgtcga ctttatcata atatacgcct tcatcataat
     1261 aatattcttc tttatcatta gcagatacaa ctatggctgt tgtcgtcatt gccagcataa
     1321 agaagcctac cggataccag tatggtcccc atccaaaagg ataataagaa tgataactgt
     1381 aaggataata tccaccgtaa tggtaaccat gataataatt gttatagtta ttatcataga
     1441 aattatcccg tctgtcaccg cgtgtgtcct gacgatcatc ctgtctgtca ccgcgtttgt
     1501 cctgcctgtc atcctgcctg tcaccacggt tatcttgtct gtcatttctg tcattacgtt
     1561 ctttgtcatt tcggttactc tgatcacgtt tattatcttt tgtatcgttt gttcttatgg
     1621 attcacggga atttctgttt gtattgtctc tctttttatt atcacggttt atctttttgt
     1681 tatttgtgtt aactttattt ctattggtat tatttttcct ggtagtagta tttcggtttg
     1741 tattgaaatt tctgttgcct ccgtttatag ttcgaccgct tctgtttcct gaatatccgg
     1801 atctcccacc acggcttccg cctctcattc tctgagcttc tgcatctgca ggagtaaatg
     1861 taatcgctac taaagagact aatgagaata tgaatattga tagtaaaatt tttgcattca
     1921 taattttctt attttattaa ttttattttt tccgcgtcta ttggaggtgt gaattcaaac
     1981 atatcatcat ttaaaatttc atcctgtatc cagtctgcaa agacaatcct gtatctcgga
     2041 gtgtctggtt ttaaaatata atctatcatc atttttaaag gctggaattt tccatcagac
     2101 gaaacccaga tctgaaatgt aatgtcttcc ttagtcccgg ctatatgata gctttccttt
     2161 tcaccgacca tggtaagtcc gagacaaata aggttatctg aattctcaag tatgtgatct
     2221 acaaaatcag gataaaatac atcagcaccc ggaaattcga ctccataagt actgctgaca
     2281 gaatctataa attcaataag tgtcatcgtt gtgggcattg atgcataacg gtttttatct
     2341 attgaataaa gaagaaataa atttccgttg taaaaaattt tattatgacc cttgtcacct
     2401 ttcttttcaa taaaaagttt atccgggcct ttcaaaaaga cttttccgaa ttccgaatgt
     2461 gtaaccattc cgtattcttc gctgttttta tcgaactccg aatagtattt aaaactgcag
     2521 gtttcaagtt cacttattct gtcactcata ctgtcaagta tagatatagc gagagaatca
     2581 aactgtgaat atgacttctc aggaaatgct gaactgcaaa atgagacaac taaaaaaaga
     2641 agaatggcta gtttcaattt catacattgt ttttatgtca aactaaaact tatttcttaa
     2701 aaccttaata caaaaattta tacacataaa tctttaaaaa tcactttccc tttgtgtctc
     2761 ccatgattgc aaatgttaaa acaattttac aaagcaggca gacagtaagg gaaaatacaa
     2821 ttattgtttt ttacttagat ggctgaatgc tgtacatatt cagacctaat ttttcagaaa
     2881 gaaattttat tactaattgt gctttgacac tgttgccgac atcatctaaa ggaggtgaga
     2941 ttactccgat ccctaatact ccgggagcta ctgcaatgat tccaccgccg actccgcttt
     3001 tacccggcat tccagtttca taaagccaga ttcctgaatc atcataaagt cctgctgttg
     3061 ccatcaccgg aagtacatgc tctactgtct tacttgatat tacagtttta cctgtaaccg
     3121 gattgatacc gccgtttgat aaagtagcag ccatcattcc aaggtctttt gcatttacat
     3181 ttattgcaca tgctttagta taaatgtctg tagtctttac aggatcgaaa tacattcttt
     3241 catatgctaa taacaaccag gctaatgcct gatttctttg attggttgca gcttcacttt
     3301 cataaaccgg ctgattcact gttaaggttc ttcctgcaaa atcactatac atactaataa
     3361 tgttattcca tttctcttct tccgtagttc cttttataag actggtagta gcaagtgcac
     3421 cgggatttac acaaggattc atttcatttc cctttttcca ttcaatcggt tcgatagcat
     3481 tgaacttcat tcctgttgcg tcaactccaa ccttatctct gatagcttca aaaccgagat
     3541 cttctatgac cattgccatt gcaaagactt tttcaattga ctgcatagaa accatatcat
     3601 caatatcacc gaattcaatg acatcaccat ctacagtgac tactgatatt ccgaaaatat
     3661 tcggatcgac ctcagcaagg gccggaatgt aatcagcgtt tttaccatca gttacatttt
     3721 taaatttttc gtaagcttct tttactgctg attcaattgt ttcttttgta agcttctgag
     3781 catacaactg cccggaaatc aaaaaagtac tgaaaattac tacgaggagt aactttacta
     3841 ctaagttttc tttttgataa ttcatgtttg ttttttatgt taaattgatt taaataaaat
     3901 tttactttta aacttaaaaa cttaatttta attaatattc tttaactttt atcaaattga
     3961 agtaagcctt ctttatcaat aagaaagcta cttcaatttt tttaaataat actttttttc
     4021 aaaatcgtac taaatgcttt gaattccgtt caattgctta aatatatact tccagatcgt
     4081 tacacctttt cttttaaata aaatttactg atgtatattc ctaatcttat acttttgttt
     4141 gaagtcattc aaaaaagtat attaacatat ttctttctct gttttatttt tcattgatca
     4201 aatagtaatt tcatttcaga tcaaataaat aaaatttttt tattagaatt tccagatctg
     4261 tgagaaatta tatttgaatg caaatcttac ttctgaagta gtagaatgaa atccgtcact
     4321 aaaattatct cttcttccga aattatattc tactccggcc attacgtttt caacaggata
     4381 atacaataga tttacaagtc cgtattgacc ttgcttgtaa gcattcggtg tctgaagatc
     4441 ggaattatca atagtttcaa cagaatatcc aaatgaactg gagaacagct tgctccagtt
     4501 tacatccata aaagcagtaa taccccagac aggaagagct tttcctttaa aaggctgtaa
     4561 tgaatctcct gggttacttt gaatacctat atctacaggt gcatcattca tataattctg
     4621 cattccttct ccatacacac cctgaatttt aaatattgct ttctttccaa tgttaatatt
     4681 cgagcttaat gttccacccc atcttactgc acttccgttt aattgaaatc tagctgtatc
     4741 tgaaacatcc ctccatttta tacttcccac aattccgcca agctgaatat atccaaagtt
     4801 tcctgtatat ctataatgtg cagtaaaatc cggcacattg aataaaggtt ttacatttgt
     4861 tagttctact ctgtttgaat aaattcctcc gtcaccactt gctcccggct gttcaagtgc
     4921 cattgcaaga ttatcctgtt tagtcattat aggagtataa cgtaattgta tatttctgta
     4981 aaaaatcatt cccgtaggtc cccagtattc gagactgttt ggaaatacat caatgtccat
     5041 aaaggtactg ttggtctgac ctgccaaaaa tttacccagt tgaccgtaaa tttgtctggg
     5101 acggatcgta gtttgtcctg catcagcacc tacaccataa agatcaaatt caaatacagt
     5161 ttttaaatca ccaagacctg ttggaacagt actctttaca ccaaatcggg attgtctcac
     5221 actataaaat aattttccgt ccggcgcaaa ttcaccttta aaagatggta atctggtgac
     5281 tctgagtgca tcataataat caggattaat tgtcttaaaa ttatatcctg cttcggccat
     5341 ggtaaatcca taaacttcaa atgacttgtc attctgtgct ttaatattaa aattgctgaa
     5401 acaaaaaatc atcagcaata caactgctgt tacaaatctg gaaagtaatt tagttttcat
     5461 aaaggtttaa attaattttt attgttaagt tgtatttgaa aaaaatattt atgctttttt
     5521 gaatttccgt aaatatgata attatgtaaa caaaaatata tcaaaaattg gttttttcaa
     5581 aaggttatta aaacctatct tacttttgat aaaattggat tttcgttaat tttttgagtt
     5641 tttttttgaa ttattctcta atcttactct aattgaaaat taaaaaaaca ttaaaattta
     5701 caacccttta caactccttg acagatagtg aaaaatttga attttatgaa tacctaaaat
     5761 catctttatc acctaagctt catattccaa aatcaattat tgaaaatttt aaaccgggtg
     5821 taaatatatc tgaactccag cttaataatc taagaagtca aaccatctgg aacattaatt
     5881 ctcagctgtc aaaagctctg aaaatgtata tcgcgataaa agaattatca aacgacccgg
     5941 acctcaatta caggctcata agagttcagt tgaaaaaaag aaatctggat aggctgctta
     6001 caaataaatg tgtcacaaga attaatgagc tgaataaatc aaagatacag gggaattctt
     6061 tcagaaatct tgcagatgca tatatggatt atttacaaac tttactttca gatgatgata
     6121 aaaagaagca gttagaagta tatgaattgt ataataaata tttttcacta tcaatgatct
     6181 ttgaattaat tattttgaga tttaataatg aagtaacttc taactctcaa aaagaatcaa
     6241 aaatttattt tttaaatgat ttttacaatt gtatagattt tggcaaaata atggaactgt
     6301 taaaaaccaa ctttccaaat tattatccgg tgatcggaat gtggaatgac ttttacgaat
     6361 tatattcagt gaaatttgat ttagaaaaat atcatatagt gaaaaataat ttctttagta
     6421 taatggatca attttcagat gaattaaaat ccggatcatt taatgtatta actgatttgc
     6481 ttattgttaa aagagaagaa ttaggtattg atcttgacgg cgaactgttt gaaatttttt
     6541 tcaaaaaaga agcacaaggt ctgactgaag actttacatt taatagaatc gggtataatc
     6601 atttcagaga taatatactg atcgcaataa atgtaggtga gatcgaatgg gcagaatatt
     6661 tcttaaataa atattcggat acacttcctg aaaagcaaaa aaataatgac ttaaataccg
     6721 gtaaagctcg gataagtttt tctaaaggag aattcaaaga ttcaataact ttcatcggga
     6781 aactcaaaaa atcttctttc atacactaca gcgattattt cagaatttca ataaaagcta
     6841 atttcgaact ttcaaattac aatgaatgtt ttttacttgc tgaaaaattc agtgaatatt
     6901 taaaaaggaa taaaaacctt ccgagttcat ttgttaatgg ttcaaaattc tttatcaaaa
     6961 atatagcagt actttctgaa tataaaatga cttctgacag gaagcgttta aaaaaaattg
     7021 acatgccaaa gttacaagtt aaaaactctt ctaataaatg gttacttgat aaatttaata
     7081 ttttaatcaa atctaattct tgaattaggt gtatgacctg tagttttccc gcacatttta
     7141 tttgagtatc ttcagttata aaatttcagt tgaatatatt ttatataaat cctattatgc
     7201 gctaaattga attgcaaata ttaagatttt tttaaaaaaa atattaatta ttgcagaatt
     7261 tttttttata tggaaatata ctttcttaaa ttttaattcc agtaatgttt atagataaaa
     7321 tcccactcga ggttattttc ttagctacat tgataatatt ttttatttca atggaatttg
     7381 gctaccggat tggaaaccgc ctttcggaga gtgcaaaaaa ggtcaaggaa aagatcactt
     7441 ctgcaaatgc ttcagcagta ctgacaatgc tcggttttat gttagtattt tcatttggaa
     7501 tagtttacag ccgctttgat tcaaaaaaag aacttgtact ggatgaagca aacttaatac
     7561 gaactgcctg gctgcgttca gattttctgc aggatcagga tcgggtggaa acagaaaaat
     7621 tattaataaa ttatgtagat atccggatta acgccgcgaa tgaaaaggat ctgtcgctga
     7681 tacaaaattt attagatcaa tcaaatgaga ttcagatgaa attatggaat atcgctgttg
     7741 caaatgccag gatcgatatg aactctgata ttggagcttt atatatagaa gcagtaaatg
     7801 agatgatcaa tttgcagtcc aaaagagttg tcctcggcat tcaggcaaga ttgcctcttg
     7861 ctatatggat aatgcttttt tcacttgtca ttttcggaat gatttgtgta ggctatgaaa
     7921 ctgcgataaa ttcttccagc aaaatttcct ggctta
//
LOCUS       DAORVQ010000159         7922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5283_length_7922_cov_4.776408, whole genome shotgun sequence.
ACCESSION   DAORVQ010000159 DAORVQ010000000
VERSION     DAORVQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_7922_cov_4.776408"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..2827)
                     /gene="cphA"
                     /locus_tag="PLX80_11375"
     CDS             complement(152..2827)
                     /gene="cphA"
                     /locus_tag="PLX80_11375"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQY21434.1"
                     /translation="MKILEIKALRGANYWSNRWKKLIVMKLDIEDYEEKPSDKIKNFP
                     TRLKELIPSLQSHTCSYGYDGGFLQRVDEGTWAGHIIEHIALEMQTLAGMDTGFGRTR
                     ETKTPGVYSVVFSYLEEKAGLYTAAKSVDLFLAIAGDVPMDDLKKMLANNIQLLREIR
                     EDVRFGPSTGSIIEEAEQRGIPHIRLNDQSLVQLGYGVYQKIIQATVSCKTGFIATDI
                     ASDKSLSKKLLESMGIPVPKGFKIYDEADVHDAVESIGFPVAVKPLDSNHGKGISANI
                     NSMYDAVKAFHSAQKYSRSVIIEKSLSGNDFRALVINNKLIAVAERTPASVTGDGKQS
                     IRELIEIVNKDPRRGYGHEKILTQIKIDNMTLRMLELKGMNPDTILKKGEICFLKSTA
                     NISTGGTAIDRTDEVHPDNIFLFERIAKIIGLDIAGIDVVAPDITTPIIENGGGIVEV
                     NAAPGFRMHLAPSEGLARNVAEPVVNMLFPAGEKFRIPIIAVTGTNGKTTTTRLISHI
                     LKNVGKTVGFTTTEGVYIGNRLIQAGDNTGPVSAQMVLRDPTVDIAVLETARGGLLRA
                     GLGYDGCEIGIVLNVTADHLGLKDIDSLEDMSRVKAIVAENVFPTGYAVLNADDKLVL
                     NMRDRLECHVALFSMDENNEAVKAHTNKGGIACVYENNFITLLKGHWKLRVEKVLNIP
                     LTFEGKAAFMIQNVLAATLASYILHGVSIEDIKFGLSSFVPSVTQTPGRLNLIEVGNF
                     TVLIDFAHNPAGMEALSKFVEKLSNKNKTGVIGGTGDRRDEDIFNMGKTAAKMFNSII
                     IREDESLRGRNEGEVNKIVLDGIKSEKPDLPVKVITNEVEALNFALKKAKKNDLLVLL
                     PDNIPRAIMIVNEFRDKLNQIKIEQSDIPNMNEET"
     gene            complement(2910..3752)
                     /locus_tag="PLX80_11380"
     CDS             complement(2910..3752)
                     /locus_tag="PLX80_11380"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQY21435.1"
                     /translation="MDNLYYKEEIGSEVLIIGGAEDKLNERYILRKFVEMSGGKSASI
                     LVIPVPSDFPIATATLYKSIFEKIGVKKVSVLEANSSKEFLNLNVEEVLDAITGVFLT
                     GGDQLRLSSILGGTKFLSELKIRIKNGTVIGGSSAGAAGFSSTMIVRGEPTVQPLKDS
                     IRLCPGLGILQNIIIDQHFTQRGRINRLITAVSYNPTNLGIGIDENTAVHIRKDGILE
                     ILGSGTVTIIDGSNISYNTIADVEESEPFSVVGLNIHILSRGVRYDLNNRKPIEMVGD
                     YLQI"
     gene            complement(3814..4701)
                     /locus_tag="PLX80_11385"
     CDS             complement(3814..4701)
                     /locus_tag="PLX80_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQY21436.1"
                     /translation="MSTNPALNTIEELDEMARQIRRDIINMLVISQTGHSGGPLGMAD
                     IFTALYFNKLNINNSDISMEERDYFFVSIGHIAPVWYATLARRGFFPLEEMKTLRRIN
                     GRLQGHPAAPKGHGAPGVEIASGSLGQGLSVAVGMALGLRLDGKPNHVYCINGDGELQ
                     EGQIWEAVMTAAHHNVDNLTMIVDRNHCQIDERTEKVLKLEPLADKFIAFGWNVLHLD
                     GHNMSEILETIDKAKSMKGKPTVIIAETLMGKGVSFMEDNYKWHGIPPTTEQAGIALK
                     DLSVTKYGDFFNLEEMAEL"
     gene            complement(4729..5343)
                     /locus_tag="PLX80_11390"
     CDS             complement(4729..5343)
                     /locus_tag="PLX80_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQY21437.1"
                     /translation="MFYIYCLVFYLIGSIPTAYILVKLKYKKNLTEEGSGNIGARNTL
                     DVTQSKSDGVIVLLVDLLKGLLPSLFLIMYSELNREEMIIPLTLLVAGHNFSIWLKFK
                     GGRGLSTAAGVFIAVDFLIVLIWLAAYFLAEVFVKNVNVSTVIALLILPLAFIIFPGF
                     ILNFINPLLTGYNEQAFCLILVISVCTVIILKHIKPLREWWFIK"
     gene            complement(5350..6228)
                     /locus_tag="PLX80_11395"
     CDS             complement(5350..6228)
                     /locus_tag="PLX80_11395"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HQY21438.1"
                     /translation="MNLLKKNILIIILLIFVSLLSFNKITSHPADDYEKMKCQKKLKS
                     FDASLQELPLNMVIAEVGKSFMGTPYVAGTLDENPNSEQLTIYITGLDCVTFVENALI
                     FSRLIQSGKTDFESYKAELELIRYRNGKNTGYASRLHYFTDWIYDNEQKGIVKNITAD
                     IGGINSGKNIDFMTSHQGSYPQLVNSDELMGEMYDVESNINMREFYYIPEENISSVYD
                     KLETGDIIGITSSLDGLDIAHTGFVYKEGGNTYLMHASLKNKKVEISATDIQDYITGN
                     SKQTGIVVARVLEISK"
     gene            6290..6790
                     /locus_tag="PLX80_11400"
     CDS             6290..6790
                     /locus_tag="PLX80_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21439.1"
                     /translation="MKNNLTQPAKLTSQKVKLRKAVRSDKKSILNLVKELAEFEKLTF
                     LDDKAGSRLMKDAFSKDPPFRTLVAEADKKVIGYAFYFFTYSSFFAKKTLYLEDIYIS
                     EKYRNLGIGKKYFIELLKIARKNKCGRMEWVVLDWNENAIKFYEKLGAYELKEWKTFR
                     KIINYD"
     gene            6920..7516
                     /locus_tag="PLX80_11405"
     CDS             6920..7516
                     /locus_tag="PLX80_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY21440.1"
                     /translation="MSNINLDINSDDDLLIDSFKNGNRSAFNLIVRKYQKKIYMTIKK
                     MVLDHDDADDITQEVFIKLYRSIKDFRGESKFFTYLYRIAINYSLNHLNRNKKLRMRN
                     TEFNETNDSHSRLNGHNIHEIIDEKERSKILKKAIETLPDQQRAVFVMRYYDNMSYDE
                     ISKILNKSVGGMKANYFHALKKIGTCIKKNKIFESAHL"
     gene            7525..>7922
                     /locus_tag="PLX80_11410"
     CDS             7525..>7922
                     /locus_tag="PLX80_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21441.1"
                     /translation="MDNKTIYTKEELLELLPDFASGNISDNALSESLTNMIESDSEFK
                     EEFESVKMSLLFLDQSETENPDPAYFNNLSVRINQRIDKELSENKSSEFIENFSRLWK
                     FIIPALTVILVILYFTLRQSDSEIMVTEST"
BASE COUNT         2521 a         1537 c         1142 g         2722 t
ORIGIN      
        1 aaatcacttt catatttatc aattttaaaa aaatgataat tttatttttt aaattttaaa
       61 ttatcattca caaaaaattt aataatttct aaaaaaactt ctttactcct tcttcaaatt
      121 caaaaatgca attactttaa tatttatttt ctcaagtttc ctcattcata tttggaatat
      181 cactttgctc aatcttaatc tgattcagct tatccctaaa ttcatttact atcattattg
      241 ctcttgggat attatccgga agtaaaacta acagatcatt tttctttgct ttctttaatg
      301 caaaatttaa tgcttctact tcattagtaa ttacctttac cggtaaatca ggtttttcac
      361 ttttaattcc atccaataca attttgttta cctccccttc atttcttcct cttaaacttt
      421 catcttctct gattataata ctgttaaaca ttttagctgc tgttttaccc atattaaaaa
      481 tatcttcatc ccttctgtca cctgttccac caattacacc agttttattt ttattcgaaa
      541 gtttttctac aaatttagat aaggcttcca tacctgcagg attgtgagca aaatctatga
      601 gtacagtgaa gtttccaact tcaatcagat tcagtcttcc cggagtctga gttactgaag
      661 gcacaaatga acttaatccg aatttaatat cctctatact tacaccatgt agtatataac
      721 ttgcaagagt tgctgcaagt acattctgaa tcatgaaggc tgcctttccc tcaaatgtta
      781 atgggatatt taaaactttc tcaactctta atttccagtg acctttcaaa agagtaataa
      841 aattattttc ataaacacat gcaatcccgc ctttatttgt atgagctttt actgcttcat
      901 tattttcatc catactaaat aaagcaacgt gacattcaag tctgtctctc atatttagaa
      961 caagcttatc atctgcattc aatactgcat agcctgttgg gaatacattt tccgctacaa
     1021 tagcttttac tctcgacatg tcttccaagg aatcaatatc ctttaatcca agatgatctg
     1081 cggtaacatt caatacaatt ccaatttcac atccatcata tcctaaacca gccctaagca
     1141 atcctcctct tgcagtttct aaaacagcta tatcaactgt cggatctctt aaaaccattt
     1201 gagcagatac aggacctgta ttatctcctg cctgtattaa tctgtttcca atataaacac
     1261 cttcagttgt agtaaatcct actgtctttc ccacattctt taaaatatgc gagataagtc
     1321 ttgtagtagt tgtctttccg tttgtacctg ttactgcaat tatcggtatt ctgaattttt
     1381 cacctgccgg aaaaagcata ttcactaccg gctcagctac gtttcttgca agtccttcag
     1441 atggagcaag atgcatccgg aatccggggg ctgcatttac ttctacaatt cctcctccgt
     1501 tttcaattat aggagttgta atgtccggcg caactacatc aatacctgca atgtcaagtc
     1561 ctataatctt tgcaattctt tcaaacaaaa aaatattatc cggatgtact tcgtcggttc
     1621 tgtcaattgc tgttccgcct gtagatatgt ttgcagtcga tttcaaaaaa cagatctcac
     1681 cttttttcaa aatcgtatcc ggattcatcc cttttaattc aagcatcctt agagtcatgt
     1741 tatctatctt gatctgagtc aatatctttt cgtgaccata tcctcttcta ggatctttgt
     1801 ttacaatttc aattaattct cttatacttt gttttccgtc accagttact gatgcaggtg
     1861 ttctttcagc aactgcaatt aatttattat taatgactaa agccctgaaa tcatttcctg
     1921 aaagtgattt ttcaattata actgatctgg aatatttctg agcagagtga aaagctttga
     1981 cagcatcgta catggaatta atatttgctg atataccctt tccgtgattt gaatccagtg
     2041 gcttaactgc aacaggaaag cctatcgact ctacagcgtc atgtacatca gcttcgtcat
     2101 aaatcttaaa accttttgga acgggaattc ccatactctc taaaagtttt tttgataagc
     2161 tcttatcact tgcaatgtca gtagctataa atcctgtctt acatgaaact gttgcctgaa
     2221 taattttttg ataaactcca taaccaagtt gaaccagcga ctggtcatta agtctgatat
     2281 gcggtattcc tctttgttca gcttcctcaa ttatagatcc ggtacttggt ccaaacctta
     2341 cgtcttcacg gatttcacgc agcaactgaa tattattagc aagcattttt ttaagatcat
     2401 ccattggtac atctcctgca atggcaagaa ataaatctac cgatttagcc gcagtatata
     2461 atccggcttt ctcttcaaga taactgaata caacactata aactccgggt gtctttgttt
     2521 ctctcgtcct tccgaaacct gtatccatcc cggccagcgt ctgcatctca agtgctatat
     2581 gttctataat atgtccggcc catgtacctt catctactct ctgaagaaat cctccgtcat
     2641 atccataact acaggtatgt gactgaagac ttggtattaa ttcttttaat cttgttggga
     2701 aatttttaat tttatctgaa ggcttttcct cataatcttc aatatctaat ttcattacta
     2761 ttagtttttt ccatctgtta ctccagtaat ttgctccgcg taatgcttta atctcaagta
     2821 ttttcatata ttattaaatt aaagaaaaaa agaaaagaat ttgcttattt aaaactaaca
     2881 gaaatttgaa tcaggaagaa aatgattttt cagatctgaa ggtaatcgcc taccatctct
     2941 atcggcttcc tgttattcag gtcataccga actcccctgc ttagaatgtg aatatttagt
     3001 cctactacag aaaatggttc cgattcttct acatctgcaa ttgtattata acttatattt
     3061 gatccgtcta tgatcgttac tgttcctgac ccaagaattt caagtatccc atccttccta
     3121 atatgtactg cagtattttc atctatacca attcccagat tagttggatt ataagatact
     3181 gctgtgatta gcctgtttat tctgcctctt tgagtaaaat gctgatcgat aattatattt
     3241 tgaagtatgc ctaatccggg gcaaagtctt atggaatcct ttagcggctg aactgtcggt
     3301 tcacctctta ctatcatcgt tgaactaaat ccggctgccc cggcgctcga acctccaatt
     3361 acagttccgt tcttaatccg gatctttaac tctgataaaa attttgtgcc tccgagtata
     3421 gaactcaatc taagctgatc accaccggtt aagaaaacac ctgtaattgc atcaagaact
     3481 tcttcgacat tcagatttaa aaattcttta cttgaattgg cttcaagcac acttactttt
     3541 tttacaccaa tcttttcaaa tatacttttg taaagtgttg ctgtagcaat tggaaaatca
     3601 gaaggtacag gtattaccag gattgatgct gattttccac cggacatttc tacaaatttt
     3661 cttaatatat atctttcatt aagtttatct tcagccccac caataattaa aacttcacta
     3721 ccgatttcct ctttataata taaattatcc aaatataaga attgattatt attaaaatta
     3781 aaaaaactat ttcgaatcta cgatcaaact tttttacaac tctgccattt cttcaagatt
     3841 aaaaaaatct ccgtatttag tgactgacag atctttaagt gcaattcctg cctgctcagt
     3901 cgttggagga attccgtgcc acttataatt atcttccata aaggaaacac ccttccccat
     3961 aagagtctca gctataatta cagtcggttt tcctttcatg ctttttgctt tgtcaatagt
     4021 ctcaaggatc tcagacatat tatgtccgtc aagatgaaga acattccaac cgaatgcaat
     4081 gaatttgtcc gcgagcggct caagcttcaa tactttctca gttctttcat cgatctgaca
     4141 atgatttctg tccactatca tagtcagatt atctacatta tggtgtgcag ctgtcataac
     4201 ggcttcccat atctgacctt cctgaagctc accatctcca ttgatacaat aaacatgatt
     4261 tggttttcca tccagtctca acccgagtgc cattccaact gcaactgaaa gtccctgtcc
     4321 gagtgaacct gaagctatct ctacacccgg agctccatgt cctttcggag ctgcaggatg
     4381 tccctgaagt cttccgttga tccttctcaa tgttttcatt tcttccaaag gaaagaatcc
     4441 ccttcttgct aaagtagcgt accaaactgg tgcgatatgt ccgattgata caaagaaata
     4501 atctctttct tccatcgata tatcactgtt gtttatatta agtttattaa aatataatgc
     4561 tgtaaaaatg tctgccattc cgagcggtcc gccagaatga cccgtctgcg aaattaccag
     4621 catgttaata atatctcttc tgatctgtct cgccatttca tcaagttctt caatagtatt
     4681 tagtgcaggg ttagtactca ttttaatata tttttttaat taatagattt attttatgaa
     4741 ccaccattct ctcaatggtt ttatatgttt taaaattatt actgtgcata cagaaattac
     4801 cagtatcaaa cagaaagcct gttcattata tccagttaat aaaggattta taaaatttaa
     4861 aataaatcca gggaagataa taaatgcaag tggtaatata agtaaagcaa ttactgtact
     4921 tacattaaca ttttttacaa aaacttctgc caagaaatat gcagcaagcc atatcaaaac
     4981 gatcaagaaa tctacagcaa taaaaactcc tgctgcagtt gataatcccc tgcctccttt
     5041 gaatttcagc catatggaaa aattatgtcc ggctaccagc agtgtcaacg ggataatcat
     5101 ttcctctctg ttcaattcac tgtacattat aagaaacaaa gatggaagca atccttttaa
     5161 aagatccaca agcaggacta ttactccgtc agattttgac tgagtaacat caagtgtgtt
     5221 tcttgcgcca atgtttccag aaccctcttc tgttaaattt tttttatact ttagtttgac
     5281 taaaatataa gcggttggta tagaaccaat cagataaaaa accaggcagt atatataaaa
     5341 caacttcttc tatttactta tttccaaaac tcttgctact acaataccag tctgctttga
     5401 attacctgtt atataatcct gtatatctgt cgctgagatc tcaacttttt tatttttaag
     5461 agaggcatgc atcagatatg tattacctcc ttctttataa acaaaaccag tgtgagcaat
     5521 atcaagccca tccaatgaag aagtgattcc gataatgtca ccggtctcaa gcttatcata
     5581 aacagatgaa atattttctt caggtatata ataaaattct ctcatgttaa tattactttc
     5641 tacatcatac atttcaccca tgagttcatc actgtttaca agctgcggat aagatccctg
     5701 atgtgaagtc ataaagtcga tattttttcc cgaatttata ccgccgatat ctgcagtaat
     5761 gtttttcaca attccttttt gctcattatc atatatccag tctgtaaaat aatgaaggcg
     5821 tgatgcatat ccggtattct ttccgtttct gtatctgata agttcgagct ctgctttgta
     5881 gctttcaaag tcagttttac ctgactggat cagtcttgaa aagataagag cattttcaac
     5941 aaatgtgacg cagtcaagac ctgtaatata aattgtaagc tgttcggaat taggattctc
     6001 gtcaagagtc cctgccacat aaggagtccc cataaatgat ttaccgactt cagctattac
     6061 catatttaat ggcagctctt gtaaagatgc atcaaatgat ttcagcttct tctggcattt
     6121 cattttttca taatcatctg ccggatgtga tgttatttta ttaaatgata atagagaaac
     6181 aaatatcagt aaaataatta tgagtatatt tttttttaac aagttcattt ttttgaaatt
     6241 tttattaatt taaacattat taaacttata ttaaataaag aaatctgaat tgaaaaacaa
     6301 tttgactcag cctgcaaaac tcacatcgca aaaagttaag ttaagaaaag ctgtaagatc
     6361 agataaaaaa agtatactca atttagttaa agagcttgcg gaatttgaaa agctgacatt
     6421 cttagatgat aaagccggat caaggcttat gaaggatgcg ttttcaaaag atccgccttt
     6481 caggacatta gtagctgaag cagataaaaa agtgatcggg tatgcattct atttttttac
     6541 ttactcatct ttctttgcaa aaaaaacttt atatctagaa gatatataca tttcagaaaa
     6601 atatcgaaac cttggcatcg gaaaaaaata tttcattgaa ctattaaaga tagcaagaaa
     6661 aaataaatgc ggccgtatgg aatgggtagt tcttgactgg aatgagaatg caattaaatt
     6721 ttatgaaaag ctcggggcat atgaacttaa agaatggaaa acctttagaa agatcatcaa
     6781 ctatgattaa accatatcta ttttggaatt gtctttataa ctggtgatta gaaattgaaa
     6841 atgatcagaa gagcatcagg tgttaaaata attttaaatt cttcattata aataaaatta
     6901 attttagaga aattatatat tgagtaatat aaatctggat attaacagcg atgatgatct
     6961 gctcattgat tcgtttaaaa acggtaatcg aagtgctttc aatttaattg tcagaaagta
     7021 tcaaaaaaaa atttacatga caataaaaaa aatggttctt gatcacgatg atgcagatga
     7081 tattactcag gaagtgttta taaaacttta cagatcaata aaagattttc gaggggaatc
     7141 taaatttttt acttatttat acaggatagc tattaactat tctctcaacc atctgaaccg
     7201 aaataaaaaa ttacgaatgc ggaatacaga atttaatgaa actaacgact cacattcccg
     7261 ccttaacgga cataatattc atgagatcat tgacgaaaag gaaagatcaa aaattctgaa
     7321 gaaagctata gaaactcttc cagatcaaca gcgagcggtt tttgtaatga ggtattatga
     7381 caatatgagt tatgatgaaa tttccaagat cctgaacaaa tctgtaggcg gtatgaaagc
     7441 aaattatttt catgctttaa aaaagatcgg tacctgtatc aaaaaaaata aaatttttga
     7501 atctgcacat ttataaaata tattatggat aataaaacta tttatacaaa agaagaatta
     7561 ctggaattac ttcctgactt tgcatcagga aatatttccg acaatgcact ctctgaatct
     7621 ttaactaata tgatcgaaag tgacagtgag ttcaaagaag aattcgagtc tgtaaaaatg
     7681 tctttgctct ttctggatca atcagaaact gaaaatccgg atcctgctta ctttaataat
     7741 ctctctgtca ggatcaatca aagaattgat aaagagcttt ctgaaaataa atcttcagag
     7801 tttattgaaa atttcagcag attatggaag ttcattattc cggctctgac tgtaatactt
     7861 gtcatacttt attttacttt aagacaaagt gactcagaaa ttatggtaac agaatcaact
     7921 aa
//
LOCUS       DAORVQ010000160         7818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5412_length_7818_cov_6.223110, whole genome shotgun sequence.
ACCESSION   DAORVQ010000160 DAORVQ010000000
VERSION     DAORVQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_7818_cov_6.223110"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLX80_11415"
     CDS             complement(<1..745)
                     /locus_tag="PLX80_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQY21442.1"
                     /translation="MNLKTISRPVQKELDIFDKKFSASLKSKVALIDLITKYILKQKG
                     KKIRPVLVLLSAKLCGEINERSYIAANLVELLHTATLIHDDVVDDAKTRRGIASLNAV
                     WKNKASVLIGDYLLSKGLLTAIDNKEFEFLKLTSEAVKSMSEGELLQIQKARNFDATE
                     ETYFRIISDKTASLIKTCCKLGAMSTAENKDMVDKLGVYGENIGIAFQLTDDILDYTG
                     RKKLLGKSTGNDLKEKKFTLPLIISLKNAP"
     gene            complement(763..1551)
                     /gene="tatC"
                     /locus_tag="PLX80_11420"
     CDS             complement(763..1551)
                     /gene="tatC"
                     /locus_tag="PLX80_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQY21443.1"
                     /translation="MITDPQNDQENTDSGNGPDEMSFLEHLEELRWRIIKSAIGVIIA
                     SIVVGIFINWIMDNVLLMPATKTNPPLKLQNIKPFGQFTLYMEVIIFGGIILSIPNLI
                     YQFWKFIEPALNPGEGRYIKSIVIFSSFCFIAGNLFAYFVLLPSALEFFASFGSSIID
                     NIIAIDEYFSFVISTILAAGLVFELPMISFFLSKVGILKPEFMRKYRKHAIILILLAA
                     GILTPSPDVTSQILLAGPLFILYEISIFICKFSQKKKPAGENPA"
     gene            complement(1566..2894)
                     /gene="glyA"
                     /locus_tag="PLX80_11425"
     CDS             complement(1566..2894)
                     /gene="glyA"
                     /locus_tag="PLX80_11425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQY21444.1"
                     /translation="METNTANNIRHELHEYDKEIYDAINKETLRQAEKLELIASENFV
                     SKAVIQAIGSVLTNKYAEGYPGKRYYGGCEFVDIAEDLARDRAKELFGAEYVNVQPHS
                     GSQANMAVYFTLVKPGDKVMGMDLSHGGHLTHGSPVNFSGQLYDFTQYGINPETELLD
                     YDLIEQVAVKEKPKMITVGASAYSRNIDYKKFREIADKINAFLWADIAHPAGLIAKKL
                     LNDPIPYCHIVTTTTHKTLRGPRGGMIMIGKDFENPFGKVAPKSGRIKMMSELIDSMV
                     MPGIQGGPLMHIIAAKAVAFKEALEDDFKTYCEQVIKNAQAFANKLIAMDYKVISGGT
                     DNHLMLVDLRNKNISGKTAEEALDHVGITCNKNSVPFDDKSPLVTSGIRIGTPALTTR
                     GMNENDMEQIALMIDKIISDPKNEDVKKDVSEEVSSLTSKFKLYDDIQTG"
     gene            complement(2931..4181)
                     /locus_tag="PLX80_11430"
     CDS             complement(2931..4181)
                     /locus_tag="PLX80_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21445.1"
                     /translation="MRKLLIILALLSLFSKPSFSQVEIAGGSPYSLFGIGDILYFTSQ
                     RTYGMGITGVSLLGNYVNNLNPATVTRINATTINLNFNYGFLKSKSSTSVNNVSNGDL
                     LGINIGIPFDQSRGWVMTLGFNPYSDVNYNVGLNGNIGGQNYTQNYSGTGGLSRINVG
                     MAYSFLNEINVGLEYNFSFGEIKNQNNIDFTNANYVDTKINTQTDFQKSFLKGGFVLE
                     LGKILRIISVRSLTMGFVFQSGLNLNSSESEIYSNSISNDTISLNEGQIEIPNTYGVG
                     FSNTFNNRFTVSTDLLYQDWSNYRIYGEAVEGFQKSYRAGLGVEFLPSETNISFWGRN
                     AYRIGAFYQTSYYKVNDQSINTLGFRAGINIPISRYNSLDFAANYSFRGETENGLVKD
                     EFLNFTAGVNFGELWFIRPKDEDR"
     gene            complement(4178..5305)
                     /locus_tag="PLX80_11435"
     CDS             complement(4178..5305)
                     /locus_tag="PLX80_11435"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HQY21446.1"
                     /translation="MTYQSCENNPNDVGQGFLPTIDTTGLRFLDSEADTMNITNQNYK
                     SYVTDFVTAKFMVGRYQDYESKTLLKFTNIGVDFDSATVVSSILKLKYSNYFFKDKMG
                     NTDFDIYQALEKFNFTTVKFDSINSSNIGNVSLGSYSGVVNDSQSIDITINNQIVKDW
                     LEYAADTNHAVKNYGVLLQPRISSSTIKGFYSSSNGVELVPSLTVIYSKNNVLDTLLL
                     DFTEFASMSDAPASVIPAEHFLLQNGVAYRNIMNFDLSKLPPNVIINNATLEFTLDNS
                     LSYITDETDKSIVIGMVIDSVTKTDSIFTPAFLSDTIVYSISLNSVFQRWNSGVMPNL
                     GITLKNAFEVQNLDNFAIYSSAYSDPTKRPRLKITYTPRLQ"
     gene            complement(5405..5974)
                     /locus_tag="PLX80_11440"
     CDS             complement(5405..5974)
                     /locus_tag="PLX80_11440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQY21447.1"
                     /translation="MNKAVFLDRDGTLIEEVNYLKKKEEISIYPETIKALRNFKKSGY
                     LNIIITNQSGIARGYFSEDDLDAVHIELRKMLTADGQELIEDIYYSPFHIDGIIEKFT
                     KESNYRKPATGMIERAKTEHDLDLSSSFFIGDSYTDMLCAENAGIKKILVRTGYGERD
                     YKKCIDNKIIPDFFAGNIYEASLYILNSN"
     gene            complement(5991..7475)
                     /gene="glgA"
                     /locus_tag="PLX80_11445"
     CDS             complement(5991..7475)
                     /gene="glgA"
                     /locus_tag="PLX80_11445"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HQY21448.1"
                     /translation="MAKGFNVLFVTSEVFPYSKTGGLADISNSLPQALNSLGNDVRIV
                     SPKYGQLDERKLQIHEIKRLKDLKITANGKETKFSIKSSFIHGKNTKAQMYLLESQDY
                     FKNKGIYQNSKTKKDFPNNDERYIFFCKAAIEILEILQWKPDVIHCNDWQTGLIPAMI
                     KTIYSNNNYLKDIKTLFTIHNLAYQGNFPKSKFNKTGLPDSVLTEKGGLHFGQFSFLK
                     TGLNYADKISTVSERYAEEIRSDKEFGCGLEDVLKARKKDMVGILNGIDYSIWNPVVD
                     KLIPYRYTYQEIPLKYENKRELLEKYKIEYDEEIPLLGIISRLVDQKGFDLIEKILPD
                     LMKEDVKLIILGEGEEKYQKFLKNAEKKYKGKLIVIIGFDETLAHQIEAASDMFLMPS
                     KYEPCGLNQMYSLRYGTVPVVRNTGGLADTIVDFKKANGNGFLFNKYDSNELLKTLLT
                     AIDLFKNNKSKWNKLLRQGMSLDFSWKVSAKKYMKLYKKVSGAK"
BASE COUNT         2392 a         1450 c         1213 g         2763 t
ORIGIN      
        1 aaggagcatt tttcagtgaa atgataagag gcagagtgaa ttttttttcc ttcagatcat
       61 tacctgttga tttgccaagc aattttttcc tgcctgtata atcaagaatg tcatccgtga
      121 gctgaaatgc aattcctata ttctcaccat aaacaccaag cttatcaacc atatctttat
      181 tttcggcagt gctcatggct ccgagtttgc agcatgtctt gataagtgaa gctgttttat
      241 cagagatgat ccggaaatat gtttcttcag tagcatcaaa atttctcgct ttctgaatct
      301 gcagcagttc accttcactc atacttttta cagcttctga tgtcagctta agaaattcaa
      361 attctttgtt gtcaattgct gtcaaaagtc ctttagaaag aagatagtct ccgataagta
      421 cggatgcttt atttttccag acggcattta gagatgcaat acctcttctt gtttttgcat
      481 catccactac atcgtcatgg ataagcgtag ctgtatgaag aagctctact aaattcgcag
      541 caatatatga tctttcattt atttcaccgc acagctttgc tgaaagcaaa accagtaccg
      601 gtctaatctt ttttcccttc tgctttagaa tgtatttcgt aatgagatcg atcagagcaa
      661 ctttggattt cagagatgct gaaaattttt tatcaaagat atctaattct ttttgtacag
      721 gcctggaaat tgtcttgaga ttcaaatcaa aaaatttatt tattaagcgg gattctctcc
      781 tgcaggtttt ttcttttgtg aaaatttaca gataaaaatg ctgatctcat ataaaataaa
      841 taatggtccg gcaagcagaa tctgactcgt aacgtcagga ctcggtgtca gtatacctgc
      901 agcgagaagt attaaaataa ttgcatgttt tctgtatttt ctcataaact caggtttaag
      961 tattccaacc tttgataaaa aaaatgagat catcggcagt tcaaatacca gcccggctgc
     1021 aagtattgta gaaattacaa aactaaaata ttcatctatt gcaattatat tgtcaataat
     1081 tgacgagccg aacgaagcaa aaaattctaa agcactcgga agcaatacaa aatatgcaaa
     1141 cagatttcct gcaataaaac agaaacttga aaagataaca atgcttttaa tgtatctgcc
     1201 ttcacccgga ttcaaagccg gctctatgaa tttccagaac tgatagatca aattcggaat
     1261 acttaatata ataccaccga aaattatcac ttccatatat agagtaaact gtccgaaagg
     1321 ttttatattc tgtaacttca aaggaggatt cgtttttgtg gcaggcatca gcaagacatt
     1381 atccatgatc cagtttataa atattccgac aactattgaa gcaataatta caccgatagc
     1441 agattttatt attctccacc ttaattcttc gagatgttca agaaatgaca tctcatcagg
     1501 accatttccg ctgtccgtat tttcctgatc attttgagga tcggtgatca tattaattta
     1561 tcagattaac cggtttgaat atcgtcatag agtttaaact ttgaagtaag tgaactgacc
     1621 tcttcactta catctttttt tacatcttca ttttttggat cggaaataat tttatcgatc
     1681 atcaatgcta tttgttccat atcgttttcg ttcattcctc tggtggttag agcaggtgtt
     1741 ccgattctta taccacttgt aacgagcgga cttttatcat caaacggaac tgaattttta
     1801 ttacaggtaa taccaacatg gtcaagagct tcttctgcgg tttttcccga tatgttttta
     1861 tttctcagat ccactagcat caaatgatta tctgtaccgc ctgaaataac tttgtaatcc
     1921 attgcaataa gcttatttgc aaatgcctga gcattcttta taacttgttc acaataagtt
     1981 ttaaaatcat cttccaatgc ttccttaaat gctacagctt tagctgctat aatgtgcata
     2041 agcggaccgc cctgaatacc gggcattacc attgagtcaa taagttcact catcattttt
     2101 atccttccgg atttcggagc tactttgcca aaaggatttt caaaatcttt tccgatcata
     2161 atcatgccgc ctctaggacc acgcagagtt ttgtgagtag tagttgttac tatatgacaa
     2221 tatggtatag gatcattcaa taattttttt gcaatgagtc ctgcaggatg tgcaatgtca
     2281 gcccaaagga atgcattgat cttatctgct atttctctga attttttata atcaatattt
     2341 cttgaataag cactggcacc aacagttatc atctttggct tttcctttac tgcaacctgt
     2401 tcgatcagat cataatccag taattccgtt tcgggattaa ttccatattg cgtaaaatca
     2461 tagagctgtc ctgaaaaatt aacaggtgat ccgtgtgtaa gatgaccacc gtgagaaaga
     2521 tccattccca ttactttatc tccgggcttt acaagtgtaa aataaactgc catgttagcc
     2581 tgacttcctg aatgcggctg cacgtttaca tactcagctc cgaataattc cttagctctg
     2641 tcacgggcaa gatcctctgc tatatctaca aattcacaac cgccataata tcttttaccc
     2701 ggatatcctt ccgcatattt atttgtcaaa acagatccga tcgcctgtat cacagctttt
     2761 gatacaaaat tttctgaagc tattaattca agtttctctg cctgacgaag tgtctcttta
     2821 tttattgcat cataaatttc tttatcgtat tcgtgaagtt catgtctgat attattagca
     2881 gtattagttt ccattttata cttatttttt tgtaaaaatt ctgtttgaaa ttatctgtct
     2941 tcgtcttttg gtctgatgaa ccagagttct ccaaagttta caccggcagt aaaattaagg
     3001 aactcatctt ttacaagtcc gttttctgtt tcacctctga atgaatagtt agctgcaaaa
     3061 tccagtgagt tatatctgct gataggaata tttataccgg ctctgaatcc taatgtatta
     3121 atgctctgat catttacttt gtaatatgaa gtttgataaa acgcacctat tctgtaagca
     3181 ttcctgcccc agaagctaat attagtttcg cttggcagaa attcaacgcc aagtcctgct
     3241 ctgtacgatt tctgaaatcc ttccacagcc tcaccataaa ttctgtaatt actccagtcc
     3301 tgataaagga ggtcagtact tacagtaaat ctgttgttaa aagtatttga aaacccgact
     3361 ccatatgtat tcggtatttc tatctgacct tcattaaggc tgatagtatc attacttatc
     3421 gagttactgt atatctcgct ttcagatgaa ttcagattaa gacctgactg aaatacaaat
     3481 cccattgtaa gagatctgac acttataatt cttaaaattt tccctaactc caatacaaag
     3541 ccacctttta aaaatgactt ctgaaaatca gtctgagtgt ttatttttgt atcaacataa
     3601 ttcgcatttg taaaatctat attgttctga ttttttattt caccaaatga aaaattatat
     3661 tcaagaccta catttatttc gtttaagaag ctgtatgcca ttccaacatt tatccttgaa
     3721 agacctcctg tgcctgaata attttgagta taattctgac caccgatatt gccgtttaat
     3781 cctacattat aatttacgtc actgtatgga ttaaatccga gagtcattac ccagcctctg
     3841 ctctgatcaa atggaatgcc gatattaatt cccagcagat ctccatttga aacattgtta
     3901 acggaagtac tgcttttaga tttaaggaat ccataattaa aattcagatt tattgtagtg
     3961 gcatttattc ttgttacagt tgcaggattc agattattaa cataatttcc aagaagcgat
     4021 acgccggtta ttcccatccc gtaagttctc tgacttgtaa aataaagtat atcaccaatt
     4081 ccaaataaag aatatggaga tcctccggca atttcaacct gtgaaaaaga aggttttgaa
     4141 aaaagcgaaa gtaatgccag tatgattaat aattttctca ttgtagtcta ggtgtataag
     4201 tgatttttaa tctcggccgt ttagttgggt cagaatatgc agaggaataa attgcaaagt
     4261 tatcaaggtt ctgaacttca aaagcattct tcaaagtaat tccaagattt ggcattacgc
     4321 cggagttcca tctttgaaat actgagttta aacttatgga atacactata gtgtcggaaa
     4381 gaaatgcagg tgtaaatatg ctgtcagttt ttgttacact gtcaatcacc attccaatca
     4441 cgatactttt atcggtttca tctgtaatgt atgaaagaga attatctaaa gtaaattcaa
     4501 gtgttgcatt atttataata acgtttggcg gaagttttga cagatcaaaa ttcattatgt
     4561 ttctgtaagc aacgccgttt tgcagaagaa aatgctctgc cggaataacc gaagcaggtg
     4621 catcagacat agaagcaaat tcagtaaagt ccagtagtaa agtatccagt acattatttt
     4681 ttgaataaat tactgtcagt gacggtacaa gttcaactcc attacttgaa gaataaaatc
     4741 ctttaatagt ggaagaagat atccgcggct gaagcagtac tccgtaattt ttcacagcat
     4801 gatttgtgtc ggctgcatat tcaagccagt ctttaactat ctgattatta atagttatat
     4861 cgattgactg ggaatcattt acaactccgc tgtaacttcc aagtgaaaca ttcccgatat
     4921 ttgaagaatt aatggaatca aatttaacag ttgtaaaatt aaatttttca agagcctggt
     4981 aaatatcaaa atctgtatta cccattttat ctttgaaaaa ataatttgaa tatttcaatt
     5041 tcagtatcga agatacaact gtagcactgt caaaatcaac tccgatattt gtaaatttca
     5101 aaagagtttt ggattcatag tcctgatatc tgcctaccat aaattttgct gtcacaaaat
     5161 cagtaacata tgatttgtaa ttctgatttg taatattcat agtgtcagct tcagaatcaa
     5221 gaaatctgag accggtagta tcgatggttg gcaggaatcc ctgtccgacg tcattaggat
     5281 tgttttcaca gctttgataa gtcaaagaaa gagctataag tgaaaatatt attaatactg
     5341 aaagtttatt tttgtaattc aggatatttt tatttaaaag tttttacttt tttttgtttg
     5401 gttgttaatt ggaattcaat atatacaatg atgcctcata aatgtttccg gcaaaaaaat
     5461 caggaatgat tttattatca atacattttt tataatctct ttcaccatat ccggttctga
     5521 ccaagatttt ttttatacct gcattttcag cacaaagcat atctgtataa ctgtctccaa
     5581 taaaaaaaga actactcaga tccagatcat gctcagtctt tgctctttca atcattccag
     5641 tcgccggctt ccggtagtta ctttctttag taaatttttc tataattcca tcaatatgaa
     5701 agggtgaata atagatatct tctataagtt cctgtccgtc tgcagtcagc attttcctta
     5761 attcgatatg aactgcatca aggtcatctt cgctgaaata accccgtgca atgccggact
     5821 ggtttgtaat gatgatatta agataaccgg attttttaaa attccgaagt gcttttatag
     5881 tttcgggata tatactgatc tcttcttttt tttttaaata atttacttct tcaatcaaag
     5941 taccgtccct gtcaagaaaa actgctttat tcatttgcga agtatatctt ttatttcgct
     6001 ccggaaactt tcttgtaaag tttcatatat tttttagcag ataccttcca tgaaaagtcc
     6061 agtgacattc cctgtctgag aagtttattc catttggatt tattattctt aaacagatct
     6121 atagcagtca gtaaagtctt tagtaattca ttgctgtcat atttattaaa aagaaatccg
     6181 ttgccgtttg cttttttaaa atccactatt gtatctgcaa gaccgccggt atttcttaca
     6241 acaggtacag taccgtatct gagcgaatac atctgattca atccgcaggg ttcatatttt
     6301 gaaggcataa gaaacatatc agaagctgcc tcaatctgat gtgcaagagt ctcatcaaat
     6361 cctattatta ctatcagctt tcctttgtat ttcttctctg cattctttaa aaatttctga
     6421 tatttctctt caccttcacc aagtataatt aattttacat cttctttcat aagatccgga
     6481 agtatctttt cgataagatc aaaacctttc tgatcaacta acctgctgat aattccaagc
     6541 agcggtatct cttcatcata ttctatttta tatttctcaa gtagctccct cttgttttca
     6601 tatttcaaag gaatttcctg atatgtatat ctgtatggga taagcttatc tacgacagga
     6661 ttccatattg aataatcaat cccgttcaga atcccgacca tatctttttt ccttgccttc
     6721 agcacatctt ccagaccgca tccgaattct ttatctgatc tgatttcttc agcatatctc
     6781 tcacttacag ttgaaatttt atcagcatag tttagtcctg tctttaagaa actgaactga
     6841 ccaaaatgaa gtcctccttt ttcagtcaaa actgaatcgg gcagaccggt tttattgaat
     6901 ttggatttag gaaaatttcc ctggtatgca agattgtgaa ttgtaaaaag cgtctttata
     6961 tcttttaaat aattattatt actgtaaatt gtttttatca ttgcaggtat aagtccagtc
     7021 tgccagtcat tacagtgaat tacatccggt ttccattgca atatctcaag gatctcaata
     7081 gctgctttgc agaaaaatat ataccgctca tcattattcg gaaaatcttt ttttgttttc
     7141 gaattttgat aaatgccttt attcttgaaa tagtcctgac tctcaagaag atacatctga
     7201 gctttggtat ttttgccatg gataaatgaa gattttatgc tgaacttagt ttctttaccg
     7261 ttagcagtga tcttaagatc ttttaatctt tttatctcat gaatctgtaa ttttctttca
     7321 tcaagctgac catacttcgg agaaacaatt ctaacgtcgt ttcctaatga gttaagtgcc
     7381 tgtggaaggg aattcgatat gtctgctaag ccgcccgtct ttgaatacgg aaaaacttcg
     7441 ctggttacaa acagtacatt aaaacctttt gccattaagg atattttttc tgacaaaaat
     7501 aattattcac ataaagatta gcaatgaatt aataaaacac agatttaaaa cagttgctat
     7561 ttataaaaaa tgtatttcag gtttttaata ttatctcaca cctaatattg ttattccaaa
     7621 tgttttttaa aaaccgaata atgtttaata ttatctgtat ttttgatggt ttaattttac
     7681 ttcataagtc tcctaaaaaa attatcaaaa aataatttta gaagtttttt gtttttaaaa
     7741 aatcccgcct gtaacatatt gctgttaaga attcttgaga aattgtaaaa ataaaatatc
     7801 cttgtctgag aaatttgc
//
LOCUS       DAORVQ010000161         7800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5432_length_7800_cov_4.829567, whole genome shotgun sequence.
ACCESSION   DAORVQ010000161 DAORVQ010000000
VERSION     DAORVQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5432_length_7800_cov_4.829567"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLX80_11450"
     CDS             <1..193
                     /locus_tag="PLX80_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21449.1"
                     /translation="DIEECKNKIIYYLENENEREDIARRGQNFILENYNYHSLMKILS
                     ENLKESYYRKFEPEHNLKI"
     gene            190..618
                     /locus_tag="PLX80_11455"
     CDS             190..618
                     /locus_tag="PLX80_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21450.1"
                     /translation="MIEYIKNEQNLLAIIISADFKKEGIHFFTSNDLSQQLAYMHHPK
                     GKTIEPHVHNPVARQVQYTQEVLFLKKGKLRVDFYNDEQQYLESRVLSSGDVILLATG
                     GHGFEVLEEIEMIEVKQGPYAGDEDKTRFNSISKDKIKIK"
     gene            620..1735
                     /locus_tag="PLX80_11460"
     CDS             620..1735
                     /locus_tag="PLX80_11460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006103756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY21451.1"
                     /translation="MEFIPVNEPLLSGNEKKYLAECIDSGWISSEGNFVMELEEKFAA
                     YCGRKFGIAVCNGTAALEAAMISLDLQKGDEVIMPSFTIISCASAIIRSGAKPVLVDS
                     DPVTWNMSIDEIRKKVTAKTKAIMVVHIYGLPVDMDPLLNLADEQGLKIIEDAAELIG
                     QTYKGKQCGSFGDVSTFSFYPNKHITTGEGGMILTDDESIAERCRSLRNLCFKKGRRF
                     IHDELGFNFRMTNLQAAVGVAQFEQLEKFIEKKRYIGKKYSDDLAEIKNFQLPLANTD
                     YSENIYWVYGILLKKDSGMDAEEAMKKLAENQIGTRPFFYPMHRQPVFTKMGLFKDES
                     YPVADYLGNFGFYIPSGLGITDDQILRVSKTLKVIFN"
     gene            1739..2746
                     /locus_tag="PLX80_11465"
     CDS             1739..2746
                     /locus_tag="PLX80_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY21452.1"
                     /translation="MMKIGIIGFGYWGTNLIRNFANINGAELVKVADSRQERKQFLEK
                     LCPTAEFCNDSRQITDDKNIDAVVIATPVFTHYELAQRSLINGKHVMVEKPMSSDLKQ
                     AEELIEISEKNGKVLMVDHTYLYTGAVQKMKNLIDQKQIGNIKYFDSTRINLGLFQPD
                     VNVLWDLATHDISILNYLVKEKPSFVQASGVSHTENGIENIAYLTVKYDSGFIAHFNC
                     SWSSPVKIRMMLIGGDKKMIVFNDLEPTEKVKVYDTGYEHKTDEDKTKILVDYRAGDI
                     YIPKLETKEALASMAADFLNAIKTGEKTISDKFSGLEVLKILEASQKSIKDKGREIKI
                     Q"
     gene            2754..3887
                     /locus_tag="PLX80_11470"
     CDS             2754..3887
                     /locus_tag="PLX80_11470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY21453.1"
                     /translation="MIPIAKPFLNKEEAESAYETVMSGWVAQGPRVEEFENKFCEYTG
                     AKYSAAVSNCTNGLHLAMIASGIGIGDEVICPSLSYIATANSIKYTGAVPVFAEVSPI
                     DFNLDPAHVKSLINSKTKAILLVHQIGMPADIDEFKKICRDYNLKLIEDAACAIGSEY
                     NGVKIGGDSELACFSFHPRKVMTTGEGGMITTTNKNLIDRIKLLRQHAISITGKERHS
                     SETLTFEDHIEVGYNYRMTDIQASIGICQLDKIEYIIKERRKIAARYISELGEIESIK
                     LPVEPKGRISNYQSFAIYIKKDSGFSRNEVMQKLMIKEISVRKGVMTSHREKAYSDHK
                     PVIALPVSEDLCDNSFMIPLFVPMDDNDIKHIISSLKNIFVKK"
     gene            3888..4688
                     /locus_tag="PLX80_11475"
     CDS             3888..4688
                     /locus_tag="PLX80_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21454.1"
                     /translation="MDSIKEKKKRNSFKKFIRNLNYKNLIDPRLKFPQSKFVMYTRGR
                     TGSTLLTELLNCHPEIFCDVEIFNFLYSGSIVKFPGMYINSCSKRASAKGKKVYGFKV
                     KISQLRYEHKYDNYDEILLNLYKKDWKFIHLKRVNFLRHQLSNFISFQTNIYHLREGD
                     KDFNKKIIVDIEKLYRGVIYGEEVERIEEQNLRNIPHYTVIYEDDLLDNEKFQKTADG
                     IFQFLGLKSVPVKSDLKRITQDRLEDIIENYEEVEKLFLNTKYEKYLK"
     gene            4696..5469
                     /locus_tag="PLX80_11480"
     CDS             4696..5469
                     /locus_tag="PLX80_11480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21455.1"
                     /translation="MTKKVFDAYSHYYDLLYRDKNYKAETDYICKNINELNPEAKSLL
                     DLGCGTGRHDTEFHKRGFKTTGVELSEKMFRIAEKNKEHLSDKGKNLIFKNGDIRSLK
                     LRKKYDVIVSLFHVMNYQTKNADLDMTIKTVKKHLNTNGVFMFDFWYGPAVLQDKPIK
                     RLKIIKDDKIMIKRKSTPFLKINENIVDVSFEILVTEKRTKKESVINEIHSMRYLFIP
                     EIEMILDNNGMKLIHTEEWMSGKKLTDKSWSAFAIAKLK"
     gene            5466..6659
                     /locus_tag="PLX80_11485"
     CDS             5466..6659
                     /locus_tag="PLX80_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21456.1"
                     /translation="MKKNKEKTIVILSPKIDTFSNPTLVVLIQKLINRGYKIIYYGFD
                     QLFVPSEYRKYFEFLELPFNFYTLENNLKSFVKLVKQYVKLFVKLKLKNKVNTIICVD
                     PMGLVIGGRIKSFLSFKLVYASFEIFFEDEFFVQRKKVMKKLEMNYSKLVDIVIIQDE
                     KRETLLKSVNNFSDNTEFHRIPVSPLKMEVKETDYDIHKELNIPDDKLIVIYSGSLQN
                     WSGLNEIISLFPEKWNNEFWLIIHTHQKLNEDSDLKIEINQLNINKQNITIHEKPFYR
                     FEEYFEFLSKCNIGIATYFPNNLDIFAGKNLEAIGLSSGKFSAYMMLGIPTITTNHFI
                     YRFLNNKYKFGQIINTISELPDALNKIRTGLKEKSESCQRLYDEVLNPEMKIESLIDR
                     IESKN"
     gene            6663..>7800
                     /locus_tag="PLX80_11490"
     CDS             6663..>7800
                     /locus_tag="PLX80_11490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21457.1"
                     /translation="MKSINKINKKLLSELPAPPEGNTGWPWTKEFCFNKQSENSHNPK
                     ITIITPSFNQGKFIEQTIRSVLLQNYPNLEYMIFDGGSTDGTVEILKKYDKWISFWES
                     KKDKGQSDAINKGLEKSTGRIFNWINSDDYYNEDTFQTISEIYKSTETDLIVGNYRFF
                     EDENENNEKTIDFRLRESLEETIAFVLINQPSSFFRLEVLKELGGLNLDLNFVMDQDI
                     WKKYLYKFGQDRIKIIDSELTHFRVHSNSKTFMNEFNNEYMNIYYSIAEQSGLEKQAS
                     FIKNIYGNGFRDNYKFIFELTDINILLSKKVINSLIYYKARIAFTNEDLEMFKKCIEV
                     IDPKFLNEKQKNYLINIKIKTKIKGMKLEPLLKLWTNVFGKSRSL"
BASE COUNT         2999 a          991 c         1446 g         2364 t
ORIGIN      
        1 ggatatagaa gaatgcaaaa ataagatcat atactatctg gaaaatgaaa atgaaagaga
       61 ggacatagcc agaaggggtc agaattttat tcttgaaaat tataattacc attctctaat
      121 gaaaatactt agtgagaatt taaaagaaag ttactataga aaatttgagc ctgaacataa
      181 tttaaaaata tgattgaata tattaaaaac gaacaaaatt tacttgcaat aataatatct
      241 gctgatttta aaaaagaagg tattcatttt tttacatcaa atgatctttc acagcagctc
      301 gcttatatgc atcacccaaa aggtaaaacg atcgaacctc atgttcacaa tccggtagca
      361 aggcaagtgc agtatactca ggaagtttta tttctgaaaa aaggaaagtt aagggtagat
      421 ttttataatg atgaacaaca atatctggaa agcagggttt tgagttccgg agatgttata
      481 cttttggcaa caggcggaca tggatttgaa gtacttgaag aaattgaaat gatcgaagtg
      541 aagcagggac cttatgcagg tgatgaagat aaaacaaggt ttaattctat ttcaaaagat
      601 aagatcaaaa taaaataaat tggaatttat accagtaaat gagccgctgc tttcaggtaa
      661 tgaaaaaaaa tatctggcag aatgcataga cagtggatgg atctcatctg aaggaaattt
      721 tgtaatggag ctggaagaaa aatttgcagc ttactgcgga aggaaattcg gtattgcagt
      781 ttgtaacgga acggctgcac ttgaagcagc aatgatctct ttagatcttc aaaaaggtga
      841 tgaagttatt atgccgtcat ttacgataat ttcctgtgct tcagcaataa ttagatctgg
      901 tgcaaaacct gtattggtag attctgatcc tgtaacttgg aatatgagca ttgacgagat
      961 cagaaaaaaa gttactgcca aaaccaaagc aattatggta gtgcatattt acggacttcc
     1021 tgtagatatg gatcctctct taaatttagc tgatgagcaa gggttgaaaa taattgaaga
     1081 tgcagcggag ctgataggac agacatataa aggtaaacaa tgtggaagtt ttggtgatgt
     1141 aagtacattt agtttttatc ctaataagca tattactaca ggtgaaggcg gaatgatttt
     1201 aactgatgat gaatcgattg ctgaaagatg ccgatcttta aggaatttat gttttaagaa
     1261 aggcagaaga tttattcatg atgaactcgg gtttaatttc agaatgacaa atttgcaggc
     1321 tgctgttggt gtagcgcagt ttgaacagct tgaaaaattt atcgagaaga aaagatatat
     1381 tggtaaaaag tattcggatg atttagcgga gataaagaat tttcaattgc cgcttgccaa
     1441 tacagattat tcggaaaata tttactgggt ttatggaatt ttactgaaaa aagattcagg
     1501 tatggatgca gaagaagcaa tgaaaaaatt agctgaaaat caaatcggta cgagaccttt
     1561 cttttatcca atgcacagac agcctgtatt tactaaaatg ggtttgttta aagatgaatc
     1621 ttatcctgtt gcagattatc ttggtaattt tggattttat attccaagtg gtctgggcat
     1681 tacagatgat caaattctaa gagtttcaaa aacattaaaa gtaattttta attaataaat
     1741 catgaagatc ggaataatag gttttggtta ttggggaaca aatttaatca ggaattttgc
     1801 taacataaac ggagctgagc ttgtaaaggt agcagattcc agacaggaga gaaaacagtt
     1861 tttagaaaag ctctgtccga ctgctgaatt ctgcaatgat tcgagacaga ttacagatga
     1921 taaaaatatt gatgcagtcg taatcgctac tcctgtgttt acacattatg aacttgctca
     1981 gcggtcatta ataaatggta agcatgtgat ggtggaaaag cctatgtctt ctgatctgaa
     2041 gcaggctgaa gaacttattg aaatttcaga aaaaaacggt aaagttctga tggtggatca
     2101 tacttattta tatacaggtg cagttcaaaa aatgaaaaat ctaattgatc aaaaacagat
     2161 tggcaatata aaatattttg attcaacaag aatcaatctt ggattatttc agcctgatgt
     2221 aaatgtgtta tgggatcttg ctacacatga catttcaata ttaaattatc ttgttaaaga
     2281 aaagccaagc ttcgtacagg caagcggtgt atctcacaca gaaaacggaa ttgaaaatat
     2341 cgcatatctc acagtgaaat atgattccgg atttatagca catttcaatt gctcatggtc
     2401 atcaccagtt aaaatcagga tgatgctgat tggaggagat aagaaaatga ttgtgtttaa
     2461 tgatcttgag ccgacagaaa aagtaaaggt ttatgacact ggttatgaac ataagactga
     2521 tgaagataaa acaaaaatat tagtagatta cagagcaggt gatatttata tccctaagct
     2581 tgaaaccaaa gaagcactgg catcaatggc tgcagatttt ttgaatgcaa tcaaaacggg
     2641 tgagaagact atatcggata aattttctgg tctggaagtt ttgaaaattt tggaagcttc
     2701 tcaaaaatcc atcaaggaca aaggaagaga aataaaaata caataataaa gatttgatac
     2761 ctatagcaaa accattttta aataaagaag aagctgaaag tgcatatgag acagtaatgt
     2821 ccggttgggt agctcagggt ccgagagttg aagaatttga aaataaattt tgtgaataca
     2881 caggtgcaaa atattctgca gcagtttcaa actgtacaaa tggactgcac cttgcaatga
     2941 ttgcttcggg aattggtatc ggagatgaag taatttgtcc ttcgctgagt tatattgcta
     3001 cagcaaattc tataaaatac accggtgcag tacctgtttt tgcggaagtc tctcctatag
     3061 atttcaatct tgatccggct catgtaaaga gtttaataaa ttctaaaaca aaagccatac
     3121 tacttgttca ccagatcgga atgccggcgg atattgatga gttcaaaaaa atctgcagag
     3181 actataattt aaaacttata gaagacgcag catgcgcaat aggttcagaa tataatggag
     3241 taaagatcgg tggagattca gagcttgcat gtttttcatt tcatccccga aaagtaatga
     3301 caactggaga aggcggaatg atcacaacta cgaataagaa tttaatagac agaataaaat
     3361 tgttgaggca acatgcaatt tcaataacag gaaaagaaag acattcatca gaaactttaa
     3421 cttttgaaga tcatatagaa gtagggtata attacaggat gacagacatt caagcttcta
     3481 ttgggatatg tcagttagat aaaatagaat atattataaa ggaaagaaga aaaattgctg
     3541 cgagatatat ttcagaatta ggagagattg aaagtataaa attacctgtt gaacctaaag
     3601 gaagaatctc aaactatcag tcatttgcaa tctacataaa aaaggactcc ggcttttccc
     3661 gaaatgaagt aatgcaaaag ctgatgatca aagaaatttc cgttaggaaa ggcgtaatga
     3721 catcccatag agaaaaagca tattcagatc ataagcctgt tatagctttg cctgtttcag
     3781 aagatctctg tgacaatagt tttatgattc cattatttgt tccgatggat gataatgata
     3841 taaagcatat aataagttct ttgaaaaata tttttgttaa aaaataattg gattcaatta
     3901 aagaaaaaaa gaaaagaaat tcctttaaga aatttataag gaatcttaat tataaaaatt
     3961 taattgatcc aagattaaaa tttccgcagt caaaatttgt aatgtatacg agaggcagaa
     4021 caggcagcac tttgctaaca gaattattga actgtcaccc tgaaatattt tgtgatgtag
     4081 agatctttaa ttttttgtat tcaggttcaa ttgtgaaatt tcctggaatg tatataaaca
     4141 gttgcagtaa aagagcttca gcaaaaggta aaaaagttta cgggtttaag gtgaaaattt
     4201 ctcagttaag atatgagcat aaatatgaca actatgatga aattcttttg aatctttata
     4261 agaaggactg gaaatttatt catttaaaaa gagtgaattt tttaagacat cagctatcaa
     4321 actttatttc atttcagaca aatatttatc acctcagaga aggtgacaaa gatttcaaca
     4381 aaaaaattat tgttgacatt gaaaaattat acagaggagt gatttacgga gaggaagtcg
     4441 agaggataga agagcaaaat ttaagaaata ttccgcacta tactgtaatt tatgaagatg
     4501 atcttttaga taatgagaaa tttcagaaaa ctgcagacgg aatttttcaa tttcttggtt
     4561 tgaaatctgt tcctgtcaaa tcagatctca aaagaattac tcaagacaga ttagaagata
     4621 taatcgaaaa ttatgaagag gtagaaaagc ttttcttaaa tacaaaatat gagaaatatc
     4681 ttaaataaat tataaatgac aaaaaaagtt tttgatgcat attcacatta ttatgatctg
     4741 ctttacagag ataaaaatta taaagcagag actgactaca tttgcaaaaa tattaatgag
     4801 ttaaatcctg aagcaaaatc tcttcttgat ctcggctgtg gtacaggcag gcatgatact
     4861 gaatttcaca aaagaggttt taaaactaca ggggtagagc tttcagagaa aatgttcaga
     4921 atagctgaga aaaataaaga gcatctttca gacaaaggta aaaatcttat ttttaaaaac
     4981 ggagacataa gaagtttgaa attaaggaaa aaatatgatg taattgtttc tttatttcat
     5041 gtcatgaact atcaaacaaa aaatgctgat ctggatatga cgattaagac ggtaaaaaaa
     5101 catttaaata ctaatggggt atttatgttt gacttttggt atggacctgc ggtacttcag
     5161 gataaaccta taaaaagatt aaagattata aaagacgata aaataatgat caaaagaaaa
     5221 tctacaccct ttttaaaaat aaatgaaaat attgtagatg ttagttttga aatattagtt
     5281 actgagaaaa gaactaaaaa ggaatctgtc ataaatgaga ttcattcaat gagatatctt
     5341 ttcattccgg aaatagaaat gattttagat aataacggaa tgaaacttat tcatactgaa
     5401 gaatggatgt cgggtaagaa attaactgat aaaagctggt cagcatttgc aatcgcaaaa
     5461 cttaaatgaa gaagaataaa gaaaagacga tcgtcatttt atctccgaag attgatactt
     5521 tcagtaatcc tactttagtt gtacttattc agaaattaat taacagagga tataaaatta
     5581 tttattatgg ttttgatcag ttgtttgtac caagtgaata cagaaaatat tttgaatttc
     5641 ttgaacttcc gtttaacttt tacactcttg aaaataatct aaagagcttt gtaaaacttg
     5701 taaagcaata tgtaaagctt tttgtgaaat taaagctgaa gaataaagta aacactataa
     5761 tctgcgtcga tccaatggga ttagtcatag gaggtaggat caaatctttt ctgagtttta
     5821 aacttgtata tgcatctttc gaaatatttt ttgaagacga attctttgtt cagagaaaaa
     5881 aagtgatgaa aaaattagaa atgaattatt caaagctagt agacatagtt ataattcagg
     5941 atgaaaaaag agaaacactt ttgaaatcag ttaataattt ttctgataat actgaatttc
     6001 acagaattcc agtttcacct ttgaaaatgg aagttaaaga aacagattat gatattcata
     6061 aagagcttaa tatacctgat gataaattaa tcgtaattta ttcgggatct ttgcaaaact
     6121 ggagcggact aaatgaaatc atttcacttt ttcctgaaaa atggaataat gaattttggc
     6181 ttattataca tactcatcaa aagctaaatg aagatagtga tcttaaaatt gaaattaatc
     6241 aactaaatat caataagcag aatattacca ttcacgaaaa acccttctat cgttttgaag
     6301 aatattttga atttctttca aaatgtaata ttggaattgc aacatatttt ccaaataatt
     6361 tagatatttt tgccggtaaa aatttggaag caattggatt gtcttccgga aaattttcag
     6421 cttacatgat gcttggaata ccaacgatta caactaatca ttttatctac agatttttaa
     6481 ataataaata caagtttggt cagatcataa atactatatc ggaacttcct gatgcactga
     6541 ataagatcag gacaggatta aaagaaaagt ctgaatcctg ccaaagatta tatgatgagg
     6601 ttttgaatcc tgaaatgaaa attgaatcgc tgattgacag aattgagtct aagaattaaa
     6661 ttttgaaaag cattaataaa ataaacaaaa aattattaag tgaacttccg gcaccaccag
     6721 aagggaatac cggatggcca tggacaaaag aattttgttt taataaacaa tcagagaatt
     6781 cacataatcc aaagatcaca ataataactc caagctttaa tcaggggaaa tttattgagc
     6841 agactataag gtcagtgctg ctgcagaatt atcctaatct ggagtacatg atctttgatg
     6901 gaggcagtac agatggtaca gtggaaattt taaaaaagta tgacaagtgg attagtttct
     6961 gggaaagtaa aaaagataaa ggacaatcag atgcgattaa taaaggtttg gaaaaaagta
     7021 ccggtagaat attcaactgg ataaattctg atgattatta taatgaagat acttttcaaa
     7081 caatttcaga aatctataag tctactgaaa cggatctcat agttggtaat tacagatttt
     7141 ttgaagatga aaatgagaat aatgaaaaga caatcgattt caggctgcga gaatctcttg
     7201 aagagactat tgcatttgta ctaatcaatc agccatcttc attttttcgt ttggaagtat
     7261 taaaagaact tggcgggctg aatttggatt tgaattttgt aatggatcag gacatctgga
     7321 aaaaatattt atataaattt ggacaggata gaataaaaat aattgattcg gaactcacac
     7381 atttcagggt tcattcaaac tcaaaaacat ttatgaatga atttaataat gagtatatga
     7441 atatttatta ttcgatagct gagcagtcag gattggaaaa gcaggcttct tttataaaaa
     7501 atatttatgg aaatggattc agagataatt ataaatttat atttgagctg acagacatca
     7561 acatattgct ttctaaaaaa gtgataaact cattgatata ttataaagcg agaatagctt
     7621 tcaccaatga ggatctggaa atgtttaaaa aatgtattga agtaatagat ccgaaatttt
     7681 taaatgaaaa gcagaaaaat tatttaataa atataaaaat aaaaacaaaa attaagggaa
     7741 tgaaactgga gcctttatta aaattatgga ctaatgtttt tgggaaaagc agatcattac
//
LOCUS       DAORVQ010000162         7762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5478_length_7762_cov_4.419359, whole genome shotgun sequence.
ACCESSION   DAORVQ010000162 DAORVQ010000000
VERSION     DAORVQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_7762_cov_4.419359"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..387)
                     /locus_tag="PLX80_11495"
     CDS             complement(76..387)
                     /locus_tag="PLX80_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21458.1"
                     /translation="MKIRLKEICHGRSGDKGDAANIGLIAYKKEDYEVIKKEVTADRV
                     KKYFEGICKGEVIRYELPNINALNFLLNNTLGGGGTVSLMLDAQGKTLASALLKMEIE
                     K"
     gene            complement(401..862)
                     /locus_tag="PLX80_11500"
     CDS             complement(401..862)
                     /locus_tag="PLX80_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQY21459.1"
                     /translation="MTELVISKKNEKWLLANGAVEYGGSHYKYIVLLHTFFFISMIFE
                     YYFVKRGSEFNIINYFFLVFFLILQTARVSVLFSLGKYWNTKIYRIPNSPLVNKGLYK
                     YFKHPNYAIVICELFAIPMIFDLYYTAIIFSVLNGVMLYYRISAENKVLEN"
     gene            complement(926..1990)
                     /locus_tag="PLX80_11505"
     CDS             complement(926..1990)
                     /locus_tag="PLX80_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HQY21460.1"
                     /translation="MPDIISVSVTELPFSILQSEMKEFAKSIFSGKFSDAERLMPVFE
                     NSEIEKRNLCVPLDYFEKKRSFADRNSDYLELTIRYSSEVINECALKAGLDLQSVTDF
                     IYVSSTGISTPSPDAYIINKLKLNKNINRYPLWGLGCAGGVSGIAKANTLAKSNPDAV
                     VLIVTSELCSLTFLNDDFSKSNFIATSLFSDGVAAVLVVGDNFKFNNENKFQLKIKDS
                     RSRIYDETLDVMGWDVTDDGLKVVFSKDIPNIVENNLKPDIEEFLCEHDLKINDIKNF
                     ITHPGGIKVINAYIKSLEIYEEKLSNTKCILSKYGNMSSATVLYVLDRFFKTGFQTGY
                     GLMTSLGPGFSSEMVLLDIE"
     gene            2118..3506
                     /locus_tag="PLX80_11510"
     CDS             2118..3506
                     /locus_tag="PLX80_11510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY21461.1"
                     /translation="MIKKFIKLMTSRREFLKQSSLIAAGTLIAPKVFSLNLSSKPAVV
                     ILGAGFSGLAAGMKLKENGFDVTILEARKRIGGRVFSHNIPDGSNDDLVIELGAEWIG
                     GSHERIKGLCNEFSLEMEDNRFKDRLIYKGKFYDPESWDWSPDWKVQFEKILADYKNL
                     TEQEKIVLDKMDWWRFLMNHDIPEKDLDIREYADSTDFGESIRFVSAYSALAEYAESN
                     EYNEMDFKVKGGNKRIAEGMAEKIGMENILLEHKATEIDSSGKVKIKCSNGKVFTCDK
                     LICTVPTFAMSKIIFTPVLSPEKIEAINALQYSRIIKSATIFKERFWKDESFSILTDT
                     YTHYFYHATKNQKSPKGVLITYAVGDKADILSKMVKEERIKVIIETLKTAMGDVSGYV
                     EDNLNYYWGDDEYSKGAYALYGKGQWFGIMPALRENTENIYFAGEHVADWQGFMEGAL
                     NSGEDAAEKIMS"
     gene            3544..4683
                     /gene="lpxB"
                     /locus_tag="PLX80_11515"
     CDS             3544..4683
                     /gene="lpxB"
                     /locus_tag="PLX80_11515"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQY21462.1"
                     /translation="MTTVPKKIFIIAGESSGDTHAASLINELKIIYSDVIVKGIGGSE
                     LSSAGAQLIYDKSEVSYIGFSSVIKNIKKLKRIIDNTCNIILEFDPDIVILVDFPGFN
                     LKIATGIRKKFKGKIVYYISPQLWAWHKSRVKIIRQCIDLMLVVFPFEVDFYKNENVT
                     AKFTGHPLIKKTNKFLSENSREISNKLTISFMPGSRKDEIKLMLPIMVKTAELFQKEF
                     DCNINIICSDNFDISYYKKFMSGHNFNLIRSEGNDYANYKTILNSCLVITKSGTSTME
                     CALIGTPFCVVYKTGAFNYAIGKRLIKVDHIAMINILLGKSAVKEFLQEKMQPEDILE
                     EGKKIINDSEYRNKMLSDFEELRKVLTEKDASKNAAILISELMSI"
     gene            4705..5298
                     /locus_tag="PLX80_11520"
     CDS             4705..5298
                     /locus_tag="PLX80_11520"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY21463.1"
                     /translation="MKKFYNFLGMRFLPFIIDLYIKTLRFKINNEPDQSGNSIFIFWH
                     SKMLTGWYIFKDKNPAALVSQSKDGEILNNLLKKWKYEVVRGSSSKGGKDAVDKLIDL
                     VKSGKNAVITPDGPRGPAGQIKNGPLIISNKCNVPIIPVNIIYKNKKTLMKSWDKFEI
                     PMPFSKCIITFGNKFNYGEYLYDDDLEDFKSKLSAEL"
     gene            5301..6341
                     /gene="lpxK"
                     /locus_tag="PLX80_11525"
     CDS             5301..6341
                     /gene="lpxK"
                     /locus_tag="PLX80_11525"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQY21464.1"
                     /translation="MNKVSVFRKILIPFSILYSFVMDIRNYLYDKKFFSICKPDVKII
                     SIGNITTGGTGKTPMVIFLAKLFLRDGKKVGIISRGYGRKSKELVIVSDGNSVIQDIS
                     LTGDEPALISFELIKEFPKSIFVVSSADRCKSANVIMNEFHPDVILLDDAFQHRRIQR
                     DKDLVMIDATDIYSNNFLYKHTLPSGLLREKIANLNRADLIILNFKESYKSETPDFYS
                     SLISDSKVVKIKYKTEYLIDHKNTILKPDKKPAVIFSGIARPESFKYTLKNIGVDIVD
                     ELIFKDHHEYSQNDIETITSNYKKDSVFITTGKDFMKINKFTDFINNFPVYYLNMEIE
                     VIDNEKLLLDLL"
     gene            6367..7059
                     /locus_tag="PLX80_11530"
     CDS             6367..7059
                     /locus_tag="PLX80_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQY21465.1"
                     /translation="MKIGISRSDTNFLRYENWLKYFEIEYRVLDYLFPEEGYKIFNEC
                     DGLILSGGVDIYPELYCDWDTKEMKGTYNPERDGFELRLVETAVQSSKPVLGICRGLQ
                     LINIFFRGSLISDLLEIRNVNHDEISPSKPRFHDVNILKDTLLYDISEIEKANVNSYH
                     HQSIDRLGEGLRINAKSEDGIIEGIEYSDRNDKSFLLGVQWHPERFEDSDISLSANIL
                     KRFISECKLENA"
     gene            7052..>7762
                     /locus_tag="PLX80_11535"
     CDS             7052..>7762
                     /locus_tag="PLX80_11535"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HQY21466.1"
                     /translation="MLNIFKYLLLVFTAVYFISCTASQNTTDSKVSFEYIKNKVNENS
                     MKLTGLDAYGEISIDSPELNNTGSITVSIKKPDSIFTKLEGPFGIDVANLLITRENFI
                     YYNVMDNRVIKGSSTQSNLSIIMKIKIEFDEIINSFSGKYYFAEEKYENAEILETENE
                     FVAVLTDKNEVKKFWIDKENYYVKKLGKYGDKGKVKLEINYENFYENDGIHFPKKITV
                     IRPEEKQSIWLTYSSEEFN"
BASE COUNT         2808 a         1138 c         1286 g         2530 t
ORIGIN      
        1 aatctatttt ttctttcgaa tcttttaatc tgtacttttg ctgttcaaac attttatata
       61 aatttaaatt taaaattatt tttctatttc catttttaat aaagctgatg caagagtctt
      121 tccctgagca tctaacataa gtgaaactgt tccgcctccg cctaatgtgt tgttaagtaa
      181 aaaattaagt gcgttaatat taggaagttc gtatcgtatc acttcacctt tacaaatgcc
      241 ttcaaaatat ttctttactc gatctgctgt aacttccttt ttaataactt cataatcttc
      301 tttcttataa gctataagcc ctatgtttgc tgcatcgcct ttgtcaccgc ttctgccatg
      361 acatatttct tttaatctga ttttcatttt ataaattttt ttaattttct aaaactttat
      421 tttcagcact gatcctgtaa taaagcatta ctccgtttaa taccgaaaaa attattgcag
      481 tataataaag atcaaatatc attggtatgg caaataattc acatatcact attgcatagt
      541 taggatgttt gaaatattta tacaaccctt tgtttacaag aggtgaattg ggaattctgt
      601 aaattttagt attccagtat ttaccaagtg aaaaaagtac tgatactctt gctgtttgaa
      661 gaatgaggaa aaataccaaa aagaaataat taattatatt gaattcactt cctcttttta
      721 caaaataata ttcaaatatc attgaaataa aaaagaatgt gtgcagcaac acaatatatt
      781 tataatgact accaccgtat tcaacagctc cgtttgccaa cagccatttt tcattttttt
      841 ttgaaatgac aagctctgtc aatctctgaa taattattat agatataatt ataggaaaga
      901 gcatcaataa gtatttatgc ctaatttatt caatatccag caaaaccatt tcgcttgaaa
      961 atccgggtcc caatgatgtc attaatccgt aacctgtttg aaatccagtt ttaaaaaatc
     1021 tgtccagaac ataaagtaca gttgcagatg acatgtttcc atatttactt agaatacatt
     1081 ttgtatttga aagtttttcc tcataaattt ccaaagattt aatataagca ttgatcactt
     1141 ttattccacc cggatgagta atgaaatttt taatgtcatt tattttaaga tcatgttcac
     1201 ataaaaattc ttctatgtca ggtttgagat tattctctac aatatttggt atatcttttg
     1261 aaaaaacaac ttttaaaccg tcatcagtca catcccatcc cattacatca agtgtctcat
     1321 cataaattct gcttctggaa tctttaattt tcagctgaaa tttattttca ttattaaact
     1381 taaaattgtc cccaactact aatactgctg caacgccgtc tgaaaataaa ctggttgcaa
     1441 taaaattact tttcgaaaag tcatcattta aaaaagtcag agaacataat tctgatgtaa
     1501 caattaaaac aactgcatcc ggatttgatt ttgccagtgt attagctttg gcaattcctg
     1561 agacaccacc tgcacagcca agtccccaca aaggatatct gttaatattt ttattcagct
     1621 ttaacttgtt gataatatat gcatcgggac tcggagtgga aattccagtt gacgaaacat
     1681 aaataaaatc agtgacgctt tgcagatcaa gacctgcttt caatgcacat tcattaataa
     1741 cttcagaaga gtatctgatg gtaagctcaa gataatctga atttctatca gcaaaagatc
     1801 ttttcttttc gaaataatct aaaggtacac ataaatttct tttttcaatt tcagaatttt
     1861 caaagacagg cataagtctt tcagcatccg agaactttcc gctgaagatg cttttagcaa
     1921 actccttcat ttctgattga agaatactaa atggcaattc tgtaaccgat actgatatta
     1981 tatcaggcaa gttgtattat tttgtaagtg caatttacta ttctttattt ccaaattgaa
     2041 tgtaaaaatt tttagaacct taagtttcta tattaaatat taggattgag tttttaaatt
     2101 tacttcaatt aggtattttg ataaaaaaat ttattaagct tatgacatca agacgtgaat
     2161 ttctaaaaca gtcatcttta attgctgccg gtactcttat tgcaccaaaa gtattttctc
     2221 taaacttaag ttcaaaacct gccgtcgtaa tactcggagc cggtttttca ggtctggcag
     2281 cgggaatgaa gctaaaagaa aatggctttg acgttacaat tcttgaagca agaaaaagga
     2341 tcggaggaag agttttttct cataacattc cagacggatc aaatgacgat cttgtaattg
     2401 aattaggagc tgaatggatc ggcggctcgc atgaaaggat caaaggtttg tgcaatgagt
     2461 ttagtcttga aatggaagat aacagattca aggacagact gatttataaa ggaaagttct
     2521 atgatccgga aagttgggat tggtctcctg actggaaagt tcaatttgaa aagatccttg
     2581 ccgattataa aaatcttaca gagcaggaaa aaatagtttt ggataaaatg gactggtgga
     2641 gatttttaat gaatcatgat attccggaaa aagatctgga cataagggaa tatgccgaca
     2701 gtactgactt tggagaatct atacgatttg tttcagcata ttctgcactt gcggaatatg
     2761 cagagtctaa tgaatacaac gaaatggatt ttaaagtaaa aggtggcaat aaacgaatcg
     2821 ctgaaggtat ggctgaaaaa atcgggatgg aaaatattct gcttgagcat aaagctactg
     2881 aaattgatag ttcaggaaaa gtaaaaatta aatgcagcaa tggaaaagtc tttacgtgtg
     2941 ataaactgat atgcactgtt ccaacatttg caatgagtaa aataatcttt actcctgtgc
     3001 tgtctcctga aaagattgaa gcaataaatg cattgcagta cagtagaatt ataaaatcag
     3061 ctactatttt caaagaaagg ttctggaaag atgaatcatt tagtattctt accgatacct
     3121 atacccatta cttttatcat gcaacaaaaa atcagaaatc acctaaaggc gttttgataa
     3181 cttatgcagt cggtgacaaa gcagatatac tttcaaaaat ggtaaaagaa gaaaggatca
     3241 aggtcattat tgaaacttta aagactgcaa tgggagatgt ctcgggatat gttgaagata
     3301 atttaaacta ttattgggga gatgatgaat attcaaaagg tgcatacgct ctttatggaa
     3361 aaggtcaatg gtttggaatc atgcctgctc tcagagaaaa tactgagaat atttattttg
     3421 caggtgagca cgttgcagac tggcagggat ttatggaagg tgctctaaat tcaggagaag
     3481 atgctgctga aaagatcatg agttaaatat atacatacaa ttttattcta attatttttt
     3541 catttgacaa cagttccaaa aaaaatcttt attattgcag gtgaatcttc aggagatacg
     3601 catgctgcat ctctgataaa tgaattaaaa attatttatt cagatgtgat tgtaaaaggt
     3661 attggcggtt cagaacttag ctctgctggc gcacaactta tctatgataa aagtgaagtc
     3721 agctatatcg gattttcatc tgtgataaaa aatataaaaa aacttaaaag aataatcgac
     3781 aatacttgca atatcatttt agaatttgat cctgacatag ttattcttgt agattttcct
     3841 ggctttaatt taaagattgc aacaggtatc agaaaaaaat tcaaaggtaa aattgtatat
     3901 tacatttcgc cgcaactctg ggcttggcac aaaagccgtg taaaaataat ccggcaatgt
     3961 attgatctta tgttagttgt ttttccattc gaagttgatt tttataaaaa cgaaaatgta
     4021 acagcaaaat ttacaggtca tcctcttata aaaaagacga ataaattttt atctgaaaac
     4081 agcagagaaa taagtaataa acttactatc agcttcatgc cgggcagccg aaaagatgag
     4141 ataaagttaa tgctgccaat aatggtaaag actgctgaat tattccaaaa agaatttgac
     4201 tgcaatatca acattatttg ctctgacaat tttgatattt cctattataa aaagtttatg
     4261 tcgggtcata acttcaatct catcagatca gaaggaaatg actatgcaaa ttataaaact
     4321 attcttaatt catgtctggt cattacaaaa tcaggtacat ctactatgga atgtgcattg
     4381 ataggtacac cgttttgtgt tgtatataaa acaggtgcgt tcaattatgc aatcggtaaa
     4441 agacttataa aagttgatca cattgcaatg ataaatattc ttcttggtaa atccgctgta
     4501 aaagaatttc ttcaggaaaa aatgcaacct gaagatatat tggaagaagg aaagaaaata
     4561 attaatgatt ctgaatacag aaataaaatg ctttctgact ttgaggaatt aaggaaagta
     4621 ctgacagaga aggatgcatc aaaaaatgcc gctatactga tatctgaatt gatgagtatt
     4681 taatatgaat aagtgtgaat taatatgaaa aagttttaca atttcctcgg aatgagattt
     4741 cttccattta taatagatct gtacattaaa acattaagat ttaagatcaa caatgaacct
     4801 gatcagtctg gaaattcaat atttattttc tggcattcaa aaatgctgac aggctggtat
     4861 attttcaaag acaaaaatcc ggcagcttta gtatcacaaa gtaaagacgg tgaaatactt
     4921 aacaatctgc tgaaaaaatg gaagtatgaa gtcgtaagag gttccagttc aaagggaggt
     4981 aaggatgctg ttgataaatt gatagattta gtaaaatccg gaaaaaatgc agtcatcaca
     5041 ccagatggtc ccagaggacc tgcaggtcag ataaaaaacg gtccgcttat aatttccaat
     5101 aaatgtaatg ttccaattat tccagtcaat ataatttata aaaacaaaaa gacacttatg
     5161 aaaagctggg ataaatttga aattcctatg ccctttagta aatgtatcat aacctttggt
     5221 aataaattca attacggtga atatctttat gatgatgatt tagaagactt taaatcaaaa
     5281 ctgtctgcgg agctttgatc atgaataaag taagtgtgtt cagaaaaatt cttatacctt
     5341 tttcaattct ttactcattt gtaatggata tcagaaatta tctttatgat aagaaatttt
     5401 tcagcatttg taaacctgac gtaaagatca tttcaattgg aaatatcact acaggcggta
     5461 caggcaaaac accaatggtg atatttcttg caaaattgtt tttacgagac gggaaaaaag
     5521 ttggcataat ttccagaggg tatggaagaa aatcaaaaga acttgtaatt gtttccgacg
     5581 gaaattctgt aattcaggat atttcactga caggagatga acctgctctc atctcttttg
     5641 aacttataaa ggaattccct aaatcaattt tcgtagtatc atcagcagac agatgtaaat
     5701 ctgcaaatgt aattatgaat gaatttcatc ccgacgttat attacttgac gatgcatttc
     5761 agcatagaag aattcagagg gataaagatt tagtaatgat tgatgcaaca gatatttaca
     5821 gtaataattt tttgtataaa catactttac catcaggatt actcagagaa aaaattgcta
     5881 atttaaacag agcagatctg atcattctaa attttaagga aagttacaaa tcagaaactc
     5941 ctgattttta ttcaagtttg atttctgatt caaaagttgt aaaaataaaa tataaaactg
     6001 aatatttaat agatcataaa aatactattt taaaacctga taaaaaaccg gcagtaattt
     6061 tctcaggaat tgccagacct gaatccttta aatatacctt aaaaaacata ggtgtagata
     6121 ttgttgatga attaattttt aaagatcatc atgagtattc acaaaatgat atcgaaacaa
     6181 taacttctaa ttataaaaag gactcagtct ttataactac gggcaaggat ttcatgaaaa
     6241 taaacaagtt tacagatttc attaacaact tccctgtgta ttatcttaac atggaaatag
     6301 aagttatcga taacgaaaaa ttattgcttg atctgcttta aatgatatat aataattata
     6361 ataaaaatga aaattggaat tagccgttcg gatacaaatt tcctgagata tgaaaattgg
     6421 ctaaaatatt ttgagataga gtatagagta ttggattatt tatttcctga agaaggatat
     6481 aaaattttta atgaatgtga cggtcttata ttatccggcg gagttgatat ttaccctgaa
     6541 ctatattgcg actgggatac aaaagaaatg aaaggcacat ataatccgga aagagacggg
     6601 tttgaattac gtcttgttga aacggctgta caatcttcta aaccagttct tggaatctgc
     6661 agagggctgc agcttataaa tatttttttc agaggaagtc tgatatctga tcttcttgag
     6721 attcgaaatg taaatcatga tgagatatct ccatcaaaac caagatttca tgatgtaaat
     6781 attttgaaag atactttact gtatgatata tctgaaattg aaaaggcaaa tgttaattct
     6841 tatcatcacc aatcaataga caggcttgga gaaggactca ggataaatgc aaaatctgaa
     6901 gacggaatta ttgaaggaat tgaatattcc gatagaaatg ataaatcatt cttactcggg
     6961 gttcagtggc atcctgaaag gtttgaagat tcggatattt ctctctctgc gaatatttta
     7021 aaaagattta tttcagaatg taaacttgag aatgcttaac atttttaaat atttattatt
     7081 agtatttaca gcagtttatt ttatttcatg tactgcttct cagaatacaa ctgattcaaa
     7141 agtttcattt gagtatataa aaaataaagt taatgaaaac tcaatgaaac ttacgggact
     7201 tgatgcatac ggcgaaattt caattgactc acctgaatta aataacactg gatcgatcac
     7261 agtaagcata aaaaaacctg atagcatttt cacaaaactg gaaggtccct ttggtattga
     7321 tgttgcaaat cttttgatca ccagagaaaa ctttatttat tacaatgtta tggataacag
     7381 ggtgataaaa ggttcttcta ctcaaagtaa tctgagtatt ataatgaaga ttaaaattga
     7441 atttgatgag atcataaatt ctttttcagg aaagtattat ttcgctgaag agaaatatga
     7501 aaatgcagag atattagaaa cagagaatga atttgtagca gtacttaccg ataaaaacga
     7561 agtaaaaaaa ttctggatag acaaggaaaa ttattatgta aaaaaacttg ggaaatacgg
     7621 tgataaaggg aaagttaaat tagaaatcaa ttatgaaaat ttttatgaaa acgacggcat
     7681 tcattttcct aaaaaaataa ctgtcataag acctgaagag aagcagtcca tttggcttac
     7741 ttacagcagt gaagaattca at
//
LOCUS       DAORVQ010000163         7644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5610_length_7644_cov_5.848728, whole genome shotgun sequence.
ACCESSION   DAORVQ010000163 DAORVQ010000000
VERSION     DAORVQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_7644_cov_5.848728"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLX80_11540"
     CDS             complement(<1..696)
                     /locus_tag="PLX80_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21467.1"
                     /translation="MKNFLLVIIIIITAFSDLTFAQNYNWITPNKTYLKLYIADDGIY
                     RISKSDFTNAGINTNNIDPRTVKIYNLGEQKPVYFRGESDGFFNDSDYVDFYGSRNYG
                     GLSNVYNADNQVIFSTNEWYDLNSDTNVYWIEWGGANGLRMVNSSYNAPVNYQQNYSE
                     QILHLEKDKIYWIGQATNANDFSNFSNERYLGESWYWNLLYNGQTLSDTFSLPLLNRN
                     SVNVSLRIFAYPQS"
     gene            complement(846..2192)
                     /locus_tag="PLX80_11545"
     CDS             complement(846..2192)
                     /locus_tag="PLX80_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY21468.1"
                     /translation="MKKYLYILFFLLLCSTLSAQLPNHNIHLIANLNNYPAYSALWGY
                     TDTDGNEYAILGCSNGTSIVNITDSSNVHEVGFIPGPISGWREMKTYSHYAYVVSEGT
                     NSRLQIIDLQYLPDSVNLVRTWSYSGYTYTHAISQSGPYLYLSGGNASPNGGVQIIDV
                     TDPVNPVRRGFNSERYVHDCRILNDTIWTCNINNQRVSIINSGNKDNPTEIRNFLTLQ
                     GFPHNCAITDDRKYLFVTHENEDPGVLEIYNIEDLDNITYIRNWQPTNITTSITHNVE
                     IYGNYAIVAHYTSGVRILDISDPVNPEEVAWYDTRPQDNSTMYEGCWAVYLFPSGKII
                     ASDISNGLFVLRATGLTGINNFVNEVPENFYLHQNFPNPFNPETSIKFEIPVQGNVLI
                     ELYNSKGEFISEIVNGNKTSGSHTVHFNAASLSSGIYFCRMNFNGSGRSIKISLIK"
     gene            complement(2315..4042)
                     /locus_tag="PLX80_11550"
     CDS             complement(2315..4042)
                     /locus_tag="PLX80_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21469.1"
                     /translation="MKILQKFSISFTAILAFILINAFITNEATASHFRYGHLTYTKNP
                     ANPLEVTFTLTDAFRRSSYGAPVVGSIFTESTGATSLIFGDATSTPTLRYVVISIDIA
                     NDWLLARALQPGDDTKESITHVYPAGGGPYQAEIFSSARTGVEINNPNQSYRVSTIVD
                     VTNGNNSPISTLPVIVNLVQSPASMFAVPAIDPDAGTTLKYRFATLAEMGGVSQNPPS
                     AGGFPATIDRVTGIVTWNTTGAVLGGLYSCQIIIEDRSSADTNILRTRVAVDFLILIN
                     NACPNPNFPTFVAPSPTCGTVFAGAPGTPISFDITASDLDPGNVLLNSTGLPPGATMT
                     PVLPQQGNPVSSQFNWVAVAGVYVVSFTATDSCGAQTICTYSFDIVLPVELSSFTSTV
                     TANDVTLNWSTITETNNSGFNIERASYVNGVLTDWNTLGNVQGNGNSNTVHSYSFTDK
                     NLGAGNYAYRLKQIDFNGNFEYHNLSSDVNIGLPTRFELMQNYPNPFNPSTKISFELP
                     TDGTVKLSVFDNSGKQVQELVNGHKNSGYYSVDFNGSNLASGVYYYKLEFSSGSESVV
                     KIRKMALVK"
     gene            4348..7518
                     /locus_tag="PLX80_11555"
     CDS             4348..7518
                     /locus_tag="PLX80_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21470.1"
                     /translation="MKTLIIKFLFLTFTLLIYSRLSFAQVNEQWLHRYTGPESSTDTP
                     SDIIIDSNGDFYMTGKSILSGTFSDIITVKFTADGTRQWISKFNGAANHNDLGNSITK
                     DNSGNIYITGNSRITGGQADVITVKYNSTGVEQWTKSYNGTGNFDDNGIEILYDGGNY
                     IYAAGYSFGTGTGYDFILLKYDLSGSFIWGKRWNGVSNSDDYLKKAAISADGNIILSG
                     YSYKVGESNNYTVLKYDPSGNLIWERFYNGPSSAEDELSDMVLDNSNNIFVCGRSVGG
                     IGGGYDYAMVKYNSAGTQQFVSRYNGTSLNDDAATGIALDPSGNIYVTGYSHVNSSFY
                     DFATVKYNSSGTQQWAKTFNGSENYFDKAVGIKVDASGNVGVTGNSIRQTDGTSDVVT
                     IKYNTSGDVIWNKQYNGPGDLDDIPIGIELDNSGNFYVTAQSYSYFFGLCGTSDYLSI
                     KYTSTGNSVFETRYDGSGSGIDESVDMNTDAGGNLYVTGYSYQTLTGLDYATIKYNPS
                     GVPLWVSRYDNSGFDEKPSGIEVDNSGNSYIAGSSIGPGSGYDFSVIKYNTDGFLQWE
                     KITNGSSGGNDYTAGITMDNLGNVFVTGHLLQNGSAYDIATIKYNNAGVQQWIKYYNG
                     VANLEDKAVEIISDGQGGVCVIGKIQSTGTGEDIVVLKYSSSGNLDWSAVYNNNSSND
                     NDIPSDIKLDKAGNIYVCGKSKISGNGFDVLLLKFNSAGNLIWSRLKNGTASLDDESA
                     SLSTDNNGNIYLSGSLNNTVTSADMTTIKYDFNGNLIWTRSFNGTSNSTDFATDLKTD
                     KAGFVYTTGFCNDIITGLNFCTIKYTGTGIKVWEEKYNYSDNDSDKAAAVHADNDGNI
                     VVTGSSKGIGGGLDFVTMKYNQNFLLDLTVLMQGYYNVNTLINVSDTVKVFLRNGSSP
                     YSIIDSSKSVSDASGVLLLYFLNVQNDQNYYIVTDHRNTLETWSSTPIKFESAGMVYD
                     YTNSLNKAFGNNLTLNGTKFCIFTGDVNKDDVIDITDLGIIDNASLNFLSGYIQEDVN
                     GDSVADLSDLGITDNNSFNFISTQRP"
BASE COUNT         2617 a         1196 c         1389 g         2442 t
ORIGIN      
        1 actttgcgga taagcaaaaa ttctaagaga gacattaacg gaatttctgt ttaagagagg
       61 taaagaaaaa gtatctgata aagtttgacc attatataaa agattccagt accaggactc
      121 acctaaatat ctttcattac taaaattact gaaatcattt gcatttgtag cctgaccgat
      181 ccagtaaatt ttatcttttt cgaggtgaag tatttgttca gaataattct gctgataatt
      241 aacgggtgca ttataagatg agttcaccat ccttaatccg ttagctccgc cccattcaat
      301 ccagtaaaca tttgtatctg aattcaaatc ataccattca tttgttgaaa atataacctg
      361 attatctgca ttatatacat ttgacagacc accataattt ctactaccat aaaaatcaac
      421 ataatcagaa tcattaaaaa agccatcaga ttcacctctg aaatagactg gcttttgttc
      481 acctaaatta tagatcttaa cagtccttgg atctatgtta tttgtattga tcccggcatt
      541 tgtaaaatcg gattttgaga tcctgtaaat accatcgtcg gcaatatata atttcaggta
      601 agttttattt ggagtgatcc agttataatt ttgagcgaat gtaagatcag aaaaagcagt
      661 gatgataata atgatgacaa gcagaaaatt tttcaatttc ataaattggg tttgctttat
      721 taaaataaag tatgaagtta aatattcacc tccttaaagt attataaatt attttataaa
      781 tttaaatcaa attaattatt attatttaaa attaaaactg ttatataatt tctcttttta
      841 ataaattatt taatcaaaga aattttaata gacctgccgg atccgttaaa gttcattctg
      901 caaaaataaa taccgctgct taaagaggca gcattaaaat gtacagtatg agaaccggaa
      961 gttttatttc cattaacaat ttcagaaatg aattctcctt tagaattata aagttcgatc
     1021 aacacatttc cctgaactgg aatttcaaat ttaattgatg tttcgggatt aaatggattt
     1081 ggaaaattct gatgcagata aaaattctca ggaacttcat ttacaaaatt atttattcct
     1141 gtcaaaccag tcgctcttaa aacaaataat ccatttgaaa tatcagaagc tataatctta
     1201 ccggacggaa acaaataaac agcccaacaa ccttcgtaca ttgttgaatt atcctgtggt
     1261 cttgtgtcat accatgctac ttcttcaggg tttacgggat ctgaaatgtc gaggatcctg
     1321 actcctgaag tataatgagc aactattgca tagtttccat aaatttcgac attgtgagta
     1381 attgatgttg taatattagt cggttgccag tttctgatat aagttatatt gtccagatct
     1441 tctatgttat aaatttccag tacaccagga tcttcatttt catgagttac aaataaatat
     1501 ttcctgtcat cagtaatggc acaattatga ggaaagcctt gtagtgtaag aaaatttctg
     1561 atctccgtag gattatcttt atttccagaa ttaattatag aaactctttg attattaatg
     1621 ttgcatgtcc agattgtatc gtttaatatt ctgcagtcat gaacatatct ttcagaattg
     1681 aaacctcttc tcacagggtt gacaggatca gtaacatcaa tgatctgaac tccgccattt
     1741 gggcttgcat ttccaccgct taaatataga tacggacccg attgtgaaat tgcatgagta
     1801 taagtatatc cgctgtaaga ccatgtcctg acaagattta ctgaatcggg aagatactga
     1861 agatcaatga tctgaagtct gctgttagta ccttcagaaa ctacataagc ataatgagaa
     1921 taagtcttca tttccctcca tccgctgatt ggaccgggaa taaatccaac ttcatgtaca
     1981 tttgatgaat cagtaatatt tacaatagaa gtaccattgc tgcatccaag tattgcatat
     2041 tcatttccat cagtatcagt ataaccccat aaagctgaat atgcgggata gttatttaaa
     2101 ttggcaatga gatgaatatt gtgattagga agttgtgcag acaaagtact gcacaataaa
     2161 aggaaaaata atatgtataa atattttttc aatttattaa atcaattatt atttttaaaa
     2221 aatatttaac cgaatataag atagaaaaaa ttattaaaat aaaatccctg tctgaatgac
     2281 agacagggat ttaaaatatt tttcagaaat atgattattt aactaatgcc atttttctga
     2341 tcttaacaac tgattcactt ccggatgaga actcaagctt gtagtaatag acaccacttg
     2401 caagatttga tccgttgaaa tctacagaat aatagcctga atttttatga ccattgacta
     2461 attcctgaac ctgctttccg ctgttatcaa atactgaaag cttaacagta ccatctgtag
     2521 gcagctcaaa gctgatcttt gttgatggat tgaaaggatt aggatagttc tgcattaatt
     2581 caaatctggt tggcaatcca atatttacat cagaactcag attgtgatat tcaaaattac
     2641 cattgaaatc gatctgcttt aatctgtaag cataattacc tgcaccaaga tttttgtcag
     2701 taaatgaata tgaatgtact gtatttgaat ttccatttcc ctgaacatta ccaagagtat
     2761 tccagtcagt cagaactcca ttaacataag aagctctttc tatattgaat cctgagttat
     2821 tggtttcagt aattgttgac cagttcaaag taacatcatt tgctgttaca gttgatgtaa
     2881 atgaagaaag ctcaacgggt agaactatat caaatgaata tgtgcagatc gtctgtgcac
     2941 cgcatgaatc tgttgcagtg aaagaaacaa cataaacacc tgcaactgca acccagttaa
     3001 attgactgct taccggattt ccttgttgtg gaagtacagg agtcattgta gcacccggag
     3061 gtaatccggt agaatttaat aaaacattac ccggatcaag atctgatgct gttatatcaa
     3121 atgagatcgg agttcccgga gcacctgcga acacagttcc acaagtaggg gacggtgcaa
     3181 caaatgtagg aaagtttgga ttaggacaag cattatttat cagaataaga aaatctacag
     3241 ctaccctggt tctcaggatg tttgtatcag cagagcttct gtcttcaatt atgatctggc
     3301 atgaatacag tccgccaaga acagcacctg tagtattcca tgttacgata ccagttactc
     3361 tgtcgatcgt agcaggaaat ccgcctgctg atggtggatt ttgactaaca ccacccattt
     3421 ctgcaagtgt tgcaaatctg tattttaatg ttgttcctgc atcaggatct atagcaggta
     3481 ctgcaaacat tgaagcaggt gattgtacaa gatttacaat aacaggaagt gtactgattg
     3541 gagaattatt tccgttagtt acatcaacga tcgttgagac tctgtaactt tgattcgggt
     3601 tattgatctc aacacctgtt cttgccgaag aaaatatttc agcctgataa ggtccaccac
     3661 ctgccggata aacatgagta atggattctt ttgtatcatc gccgggctgt aaagctcttg
     3721 caagcagcca gtcatttgca atgtcaatag aaataactac atatctcaat gtgggagtac
     3781 ttgtggcatc tccaaaaatg agagatgtag cacctgttga ttcagtaaaa attgaaccaa
     3841 caaccggagc accgtaactg cttcttctga aagcatcagt taaagtaaat gttacttcaa
     3901 gcggatttgc tgggtttttt gtgtaagtca gatgaccata acgaaagtgt gaagctgttg
     3961 cttcatttgt gataaaagca tttatcagaa tgaaagcaag tattgctgtg aaactgattg
     4021 aaaatttttg tagaattttc atgttgtctc cttgggttta agttagtaat gaatgttaaa
     4081 taagaacaat tttatattta gaattatatt taatttatca cgcctaaaac agaaatggaa
     4141 tattgaaaaa attaattaat ttattcccca attaataaat taattttaca atagaccata
     4201 tttaaattct ttaatcttta caaacataat agttttgatt taaaatgtca atcatgtgga
     4261 gatactcatt tttttaaaaa gtatacaatc aaaagtttac attttaaaat taaaataact
     4321 aattttgtat aatcaaatta taatgaaatg aaaactttaa ttataaaatt tttattcctg
     4381 acttttacac tattaattta ttcccgatta tcctttgcac aggtcaatga gcagtggtta
     4441 catcgatata caggtccgga atccagcact gatactcctt ctgacataat tattgacagc
     4501 aacggagatt tttatatgac aggaaaaagt attctgagtg gaacattctc agacattata
     4561 acagtaaagt ttactgctga cggtacaaga caatggatct caaaatttaa cggtgctgca
     4621 aatcataatg atttaggaaa cagcattaca aaagacaatt caggaaatat ttatattact
     4681 ggaaattcaa ggatcaccgg cggacaggcg gatgtcataa ctgttaaata taattcaact
     4741 ggagtggagc agtggactaa atcttataac ggcacaggaa attttgacga caatggaata
     4801 gagatcttat atgacggtgg aaattatatt tatgccgccg gctatagttt tggaacaggt
     4861 acaggttatg attttatctt attaaagtat gatctgagcg gatcatttat ctggggaaag
     4921 agatggaacg gagtttcaaa ttcagatgat tatttaaaga aagcagctat ttcagctgat
     4981 ggaaatatta ttctttcggg atattcttat aaggtaggtg aatcaaataa ttataccgtt
     5041 ttaaaatatg atccctccgg aaatcttatt tgggagagat tttacaatgg tccctcatca
     5101 gcagaagatg aactttcaga tatggtttta gataattcta ataatatttt tgtgtgtgga
     5161 agaagtgtcg gagggattgg aggcggatat gattatgcaa tggtaaaata taattctgca
     5221 ggtactcagc agtttgtatc gaggtataat ggtacttcgt taaatgacga tgcggcaaca
     5281 ggtatagcat tagatccttc cggtaatatt tatgtaacag gctacagtca tgtgaattca
     5341 tcattttatg attttgcaac agtaaaatat aactcatcag gaacacagca atgggcaaag
     5401 acatttaacg gaagcgaaaa ttattttgac aaagcagttg gaattaaagt ggatgcttca
     5461 gggaatgtcg gtgtcaccgg aaattctata cgacaaacag acggtacaag cgatgtagta
     5521 actatcaaat acaatacttc aggcgatgta atctggaata aacaatataa tggtccggga
     5581 gatcttgacg atatccctat tggtattgaa cttgataatt caggtaattt ttatgtcact
     5641 gcacaaagtt acagttattt tttcggactt tgcggtacca gtgattatct ttcgattaaa
     5701 tatacatcaa ccggaaactc agtttttgaa acccgatacg acggttcagg cagcggaata
     5761 gatgaatctg tagacatgaa cacagatgct ggtggaaatt tatatgtgac gggatacagc
     5821 tatcagactt tgactggact ggattatgca acaataaaat acaatccgtc tggtgtacct
     5881 ttatgggttt caagatatga caacagtgga tttgacgaaa agccatctgg aatagaagta
     5941 gataactccg ggaattctta tattgcagga agcagtattg gacctggaag cggttatgat
     6001 ttttctgtga tcaaatataa tactgacgga tttcttcagt gggaaaagat aactaatggc
     6061 agctcaggag gaaatgatta tactgccggt attacaatgg ataatctcgg aaatgtattt
     6121 gtaacaggac atttacttca aaacggaagc gcgtatgata tagcaactat taaatataat
     6181 aatgccggag tacagcaatg gataaaatat tataacggtg tagcaaactt ggaagataaa
     6241 gcagtagaaa taatttcaga cggacaggga ggagtttgtg ttattggtaa aattcagagt
     6301 accggaaccg gtgaagatat agtagtttta aaatacagca gttcagggaa tttagactgg
     6361 tcagctgtat acaataataa ttcctcaaat gataatgaca ttccatcaga cataaaatta
     6421 gataaagcag gaaacatcta tgtctgcggt aaaagtaaga tcagtggtaa tggttttgat
     6481 gttttgcttt taaaatttaa ctctgcagga aatttaattt ggtcgaggtt aaaaaacggt
     6541 acagcatcat tggatgacga atcagcatct ttaagtacag acaataatgg taatatttat
     6601 ttatcgggaa gtttgaataa tacagttacc tctgctgata tgacaactat taaatatgac
     6661 tttaacggaa atctgatctg gacaagatca ttcaacggaa catcaaattc aactgacttt
     6721 gcaactgatt taaaaacaga taaggctgga ttcgtttata ctaccggatt ttgtaatgat
     6781 ataattaccg gattaaattt ctgtacgata aaatatacag gcaccggaat aaaagtttgg
     6841 gaagagaaat acaattattc tgataatgat tcggataaag ctgctgccgt acatgcagat
     6901 aatgacggaa acattgtagt cacaggatcc agtaaaggta tcggaggcgg acttgatttt
     6961 gtaacgatga agtataatca aaatttttta ctggatctta cagtattgat gcaaggatat
     7021 tataatgtaa atactttaat aaatgtatcc gatactgtaa aagttttttt aagaaacgga
     7081 agttctccat acagtatcat tgattcttca aaatcagttt cagatgcatc aggagtactg
     7141 cttttgtact ttctaaatgt acagaatgat cagaattatt atatagtaac agaccataga
     7201 aatacacttg agacctggag ttccacacca ataaaatttg aatctgcggg aatggtttat
     7261 gattatacaa attccttaaa caaggctttt gggaacaatc tgactcttaa cggaacaaag
     7321 ttttgtatat ttacaggtga tgttaataag gatgatgtaa ttgacattac agatctggga
     7381 attattgaca atgcaagttt aaattttctc agcggttata tacaggaaga tgtaaacgga
     7441 gattcagttg ctgatctgtc tgatcttgga attacagata ataactcgtt caattttatt
     7501 tctactcaaa gaccttaaag taaaaaatta tttaaatgtt cagagccgga ctttaaagtc
     7561 cggttttttt tttaggattc attttaagag caaacaaata taaatcctga tgtaaattga
     7621 tattgtggtt tatgaacacg aaac
//
LOCUS       DAORVQ010000164         7578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5722_length_7578_cov_4.833178, whole genome shotgun sequence.
ACCESSION   DAORVQ010000164 DAORVQ010000000
VERSION     DAORVQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5722_length_7578_cov_4.833178"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLX80_11560"
     CDS             <1..629
                     /locus_tag="PLX80_11560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY21471.1"
                     /translation="EKSVRNLLNVKLYIDTIKNKNYGKYIIIFFVAVFSFSNIFGTYQ
                     LFAERKEGLGLSQAEIGYLFSFMGIIGALVQIFLIKIFQKKIGEANTLILGNFLTIFG
                     LGLIGFSQSVEVLLAITAVLSVGNGFNNTVSISLLSQNVKPDEQGTVLGINQSLSAFA
                     RFLGPLWGGFTYQYFGYQYPFITGGIIMIFATFYTYNVLKKNKPSVAV"
     gene            complement(705..1445)
                     /gene="pgeF"
                     /locus_tag="PLX80_11565"
     CDS             complement(705..1445)
                     /gene="pgeF"
                     /locus_tag="PLX80_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQY21472.1"
                     /translation="MSEINIIRSSLFEKYPEIIFGFSTKTGGVSPEPFCLNLSNAVGD
                     DPENVKVNRKFFFSELGIDEKNVTFQKQIHSYIINYSSKPCHFDGCDAIYTDVKENFL
                     AVGVADCIPVFLYDPVRKIIAGIHSGWMGTKEKILTKTIEELIKKFNTDPSDLISFIG
                     PGICQDHYEVGEEVGLLFDENVRYRSGEKYYLDLKKDNFKQLLKAGLRNENIEISDLC
                     TFKEKDLLHSYRRDGVRSGRMFGVIGLI"
     gene            complement(1462..2940)
                     /locus_tag="PLX80_11570"
     CDS             complement(1462..2940)
                     /locus_tag="PLX80_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21473.1"
                     /translation="MNETTTITLQEVETAISKLDNIEKFQDNFLIIEKFVSDYYNYSI
                     SERENQNFNASYQYISAGFNLCNNTFIRLKKIIEENQNNELKNILDQKLFETEIQMLR
                     FNSANNFTTGLFHFSNRNPGLANQFFSESEKNSKELYNKTKDSIFAIESELSKNYKHM
                     SEGLENTLTGDLIDARKNYQIAKIGFEDIIKKLQHENTDENSLLKTKIDYVCRECTNM
                     LHIANAQYYFRSKNYRLSFENYEKLISSYKQTITDFSGIDDDIDIDPDEDSDPVLESL
                     KNFYQGNYFYYTGHMNIVKGLDFQESSKWEDALESFEKVRTDWTRSTEFLLKSNIPQA
                     LTLQETLSQNEFSIDQYISSCLKDRDQLQRINELEEKVKMQQEELFNMLKPSGITVNN
                     IQDINNTVEQNVQIVQKIEYKTRENIKELLELLKTVPLNEFDKKEIESEGKELVESKE
                     KGVKFLDKAKSFTEKISGIIKNVGEIATPIIPIVKALSLMLA"
     gene            complement(3171..4508)
                     /locus_tag="PLX80_11575"
     CDS             complement(3171..4508)
                     /locus_tag="PLX80_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HQY21474.1"
                     /translation="MNTQITEDQLIEIYSEISEGNAEIKKYYPGDSPDRQPVQTVYGG
                     AQLFKSGTIKKIGELALKSFKEFGSDPQTFSKGLGLVGDDDYRKLIHSKVLKKLEREA
                     VEDFRIDFEDGFGIRPDDDEDQTAVFTAGETAIAMKENLLSPFLGIRIKSFSDEMMKR
                     AIRTLDIFITELVQKTDGKLPDNFVITLPKVTNKKQVSAFVKVLKTLEKKTGLSDGSL
                     KMEMMIETTQSIFDHNGNANLISLVNEGEGRCIAAHFGVYDYTALCDITAKYQNMQHS
                     VCDFARQMMKVTLAGTGVWLSDGATNIMPVTIHRGEDLTAEKIRENYESIYKAWKISF
                     DDIKHSLQSGFYQGWDLHPAQIPVRYAAVYDFFLESLDQASERLTNFIDKAAQATLVG
                     DIFDDAATGQGLLNYFLRAFNCGAVTKDEIIKTGLTETDLRGRSFMKIIENRK"
     gene            complement(4535..6436)
                     /gene="mnmG"
                     /locus_tag="PLX80_11580"
     CDS             complement(4535..6436)
                     /gene="mnmG"
                     /locus_tag="PLX80_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQY21475.1"
                     /translation="MKNFDIIVVGAGHAGIEASYAAARMGLSVGLVTMDVNSIGRMSC
                     NPAIGGTAKGHLVREIDALGGIMGEIADKTGIQFKMLNTSKGPAIWSPRCQSDKDLYS
                     NEAKKVIKDQENIEIIQDMVKELIVSEMKNNENGYSFIINGIKTETGLEIKCSSVIIT
                     SGTFLNAVMHTGKSNIEGGRLGEKAATGLSECMLGMGFETGRLKTGTPPRLFKETIDF
                     SVTEVQKGDDNPKPFSHKKYLKFPLQEQIDCYLTHTNEKTHEILRTGFEDSPMFTGRI
                     KGIGPRYCPSIEDKISRFSDKPRHQIFLEPETLNGNTIYMNGFSTSLPIDVQEKAAQT
                     IPGLEKVKLAKQGYAVEYDFFPTHQINLTLETKLVSGLYTAGQINGTSGYEEAAAQGL
                     MAGINAALKIKKEDPFILKRSEAYIGVMIDDLINKCPDEPYRMFTSCAEYRLVLRQDN
                     ADRRLMKYGYKFGLIDNNMMNYLNDKTSLISDGIEFFRKNTISPKNMNETLIRKNSKE
                     LPQHEFMYNVIKRSEVTIKDMLDLKSFDDNELISKIRDNEDAANQIEIEIKYKGYIDR
                     QDEQISNFIKNETIRIPEDFKFEKIKSLSTEALEKLQKIKPTSIGQAMRISGVRPSDI
                     SAVLIYMRG"
BASE COUNT         2236 a         1369 c         1103 g         2870 t
ORIGIN      
        1 aagaaaaatc agtaaggaat cttttaaatg ttaagcttta tattgatact attaagaata
       61 aaaattatgg gaaatacata atcattttct ttgtagcagt attttcattt tcaaatattt
      121 tcggcaccta tcagcttttt gcagaaagaa aggaagggtt gggactcagt caggcagaga
      181 taggatattt gttttctttt atgggtataa taggagcact tgttcagatc tttttgataa
      241 agatttttca gaaaaaaatt ggtgaggcaa atactcttat tctcggaaat tttctgacaa
      301 tattcggatt gggacttatt ggattttcac agtcagttga agttttgctt gcaataacag
      361 cagtcttatc agttggaaac ggattcaata atactgtatc gatcagtctt ctttcgcaga
      421 atgttaagcc tgatgaacaa gggactgtac ttggaataaa tcagtctctg agcgcgtttg
      481 ccagatttct cggaccgctg tggggaggat ttacatatca gtattttgga taccagtatc
      541 cgtttattac cggtggtatc ataatgatat ttgcaacttt ttatacctac aatgttctca
      601 aaaaaaacaa accttcagtt gcagtgtgat ctgtttattt ttctcaaaaa ttttttaatg
      661 aaaaatctga gataaaatca tacaatttct caaacattca aaggtcatat caatccaata
      721 accccaaaca ttcttcctga tcttacaccg tctcttctgt atgaatgcag cagatctttt
      781 tctttaaatg tacagaggtc agagatctct atattttcat ttctcaaacc tgcttttaaa
      841 agctgtttaa aattatcttt tttcagatcg aggtaatatt tttcacctga tctgtatctt
      901 acattttcat caaaaagtaa tccgacttct tctcccactt cataatggtc ctgacaaatt
      961 cccggaccga taaatgagat cagatctgat ggatcagtat taaacttttt aatgagttcc
     1021 tcaattgtct tagtaaggat cttttcttta gttcccatcc atcctgaatg aatgccggca
     1081 ataatttttc taaccgggtc atataagaat actggaatgc agtcagctac accgactgca
     1141 agaaaatttt ctttaacatc agtataaatt gcatcacatc cgtcgaagtg acatggttta
     1201 gatgagtaat ttataatgta agaatggatt tgtttttgaa aagttacatt tttttcatca
     1261 ataccaagtt cactgaaaaa aaattttctg ttcactttta cattttcggg atcatcacct
     1321 actgcattgc ttaagttaag acagaaaggt tcaggtgata caccgcccgt cttagtactg
     1381 aatccgaaaa ttatttcagg atatttttca aataaagaag accggattat gtttatttcc
     1441 gacacgtttt aatattttat attaagctaa cattagtgaa agtgctttta caattggaat
     1501 tataggtgta gcaatttccc ccacattttt tattattccg ctgatctttt ctgtaaaaga
     1561 ttttgcctta tctaaaaatt ttactccttt ttcttttgat tcaacaagct cttttccttc
     1621 agattcaatt tcctttttat caaattcatt taatggaaca gttttaagta attccaaaag
     1681 ttcttttatg ttttcacggg ttttgtattc gatcttctgc acgatctgaa cattttgttc
     1741 taccgtgtta ttaatatcct gaatattatt tactgttatt cctgatggct tcagcatgtt
     1801 gaataattct tcctgttgca ttttcacttt ttcctcaagc tcgtttattc tttgcagctg
     1861 atctctgtct ttcaaacatg agctgatata ttgatctatg gaaaattcat tttgacttaa
     1921 tgtttcctgt aatgtaagcg cttggggaat attcgattta agcagaaact cggtagatct
     1981 tgtccagtct gttctaactt tttcaaaaga ttcgagagca tcttcccatt tacttgactc
     2041 ctgaaaatcc agacctttta caatattcat atgacctgta taataaaaat aatttccctg
     2101 ataaaaattt tttaatgatt ctaataccgg atcagagtct tcatctggat caatatctat
     2161 atcatcatca attcctgaaa aatctgttat agtctgttta taagatgaga ttaatttttc
     2221 ataattttca aaggaaagtc tgtaattttt tgatctaaag tagtactgag catttgcaat
     2281 atgcagcata ttagtacatt ccctgcagac ataatctatt tttgttttta ataaactatt
     2341 ttcatcagta ttttcgtgtt gaagtttttt gataatgtct tcaaatccta tctttgcaat
     2401 ctgataattt tttcttgcgt cgataagatc tcctgttaaa gtattttcaa gaccttccga
     2461 catgtgttta taattttttg acagctctga ctcaattgca aatatgctgt cttttgtttt
     2521 attatataat tcttttgaat ttttttcact ttcagaaaaa aactgatttg cgagaccggg
     2581 atttctgttt gagaaatgga acaatcctgt tgtaaaatta tttgcagaat taaatctcag
     2641 catttgaatt tcagtttcaa acaacttctg atccagtata ttttttagtt cattattttg
     2701 attttcttca atgatctttt ttaatcttat gaatgtatta ttgcaaagat tgaatcctgc
     2761 tgatatgtac tgataggaag cgttgaaatt ttgattttcc ctttcggaaa tactgtagtt
     2821 ataataatct gaaacgaatt tttctattat taaaaaattg tcctgaaatt tttcaatatt
     2881 atcgagttta gaaatcgcag tttctacttc ctgtaatgta attgttgtgg tttcattcat
     2941 aaaagtattt tttttaaatt aaatataatg gaattaaaat ggtatgtatc aattgcgggg
     3001 aattaaaata tggagtttac tttttttctt atcaaatctg acacgtttgt ctgattgtat
     3061 cgtaatcgaa tcctgagttt ataaaacctg tctggtttca aaacttaagt ccaatctcaa
     3121 atcaaattca aaaacctgaa tttattacca aataaaatct ataccggaac ctacttcctg
     3181 ttctctatta ttttcataaa tgaccgtcct ctaagatctg tttccgtcaa tccggtcttt
     3241 attatctcat cttttgttac tgcaccacaa ttaaatgctc tcaaaaaata gttcagtaaa
     3301 ccctgccctg ttgcagcatc atcaaatata tcacccacta atgttgcctg tgcagctttg
     3361 tcaataaaat ttgtaagtct ctccgaagcc tggtccaaac tttcgagaaa aaaatcataa
     3421 actgctgcat atctcacagg tatctgtgca ggatgaagat cccagccctg ataaaatcca
     3481 ctttgtaatg aatgtttaat atcatcaaaa gagatcttcc atgctttgta aatactttca
     3541 taattttcgc ggatcttttc tgcagtgaga tcctcacctc tgtgaatcgt cacaggcatt
     3601 atgttcgtag caccgtctga caaccaaaca cctgtccccg ctaatgtcac cttcatcatc
     3661 tgtcttgcaa agtcacatac tgaatgttgc atgttctgat atttcgctgt gatgtcacaa
     3721 agtgctgtat aatcataaac tccaaaatgc gctgcaatgc atctaccttc accttcattt
     3781 accaaagaga tcagatttgc atttccattg tgatcaaaaa ttgattgagt tgtctctatc
     3841 atcatttcca ttttcagact tccgtcagaa agaccggttt tcttttcaag tgttttcaga
     3901 actttcacaa atgctgacac ctgtttttta tttgttacct ttggtaaagt aattacaaaa
     3961 ttatctggaa gttttccgtc agttttttga acaagttcag ttataaaaat atcaagagtt
     4021 ctgattgctc tcttcatcat ttcgtctgaa aaggatttaa ttctgatccc caaaaatggt
     4081 gaaagcagat tctctttcat tgcaattgca gtctcacctg ctgtaaaaac agcagtttgg
     4141 tcttcatcat catcaggtct tatcccaaat ccatcttcaa aatctattct gaaatcttca
     4201 actgcttctc tttcaagttt ctttaaaacc tttgaatgta tcagctttct gtaatcatca
     4261 tcacctacta aaccaagccc tttagaaaaa gtctgaggat ctgaaccaaa ttctttaaaa
     4321 gattttaaag caagttcacc aatctttttt attgtgccgg acttaaataa ttgagctccg
     4381 ccataaacgg tctgaaccgg ctgcctgtca ggagaatctc ccggataata ttttttaatt
     4441 tcagcatttc cttctgaaat ttccgaataa atttctataa gttgatcttc tgtgatctgt
     4501 gtattcataa tttttaaaat ataataaaat tttattatcc gcgcatatat atcaatacag
     4561 cagatatatc tgatggacgc acacctgaaa tcctcattgc ctgccctatt gaagtaggct
     4621 tgattttctg aagcttttct aatgcctctg ttgataatga ttttattttt tcaaatttaa
     4681 aatcttcagg aattctgatt gtttcatttt ttataaaatt acttatctgt tcatcttgtc
     4741 tgtcaatata tcctttatat ttgatctcaa tttcaatttg attggctgca tcttcattat
     4801 ctctgatttt tgaaattaat tcattgtcat caaatgattt cagatcgagc atgtccttta
     4861 ttgtaacttc acttcgttta atgacattat acataaattc atgctgtggt aattcttttg
     4921 aattttttct gatcaaagtc tcattcatat tttttggact tatcgtattt tttctaaaaa
     4981 attcaatacc atcagatata agtgatgttt tatcattaag ataattcatc atattattat
     5041 ctatcaaacc aaatttataa ccatatttca tcagtcttct gtcagcatta tcctgtctga
     5101 gcacaagtct gtattctgca catgacgtaa acattctgta aggctcgtcc ggacatttat
     5161 ttatcagatc atctatcatc acaccgatat atgcttcact tcttttgaga atgaatggat
     5221 cttctttttt aattttcaat gcggcattta tcccggccat aagtccctga gctgcagctt
     5281 cttcgtaacc tgaagttccg tttatctgac ctgcagtata aagaccggaa accagttttg
     5341 tctccaatgt aagatttatc tgatgagtcg gaaagaaatc atattcgact gcatatccct
     5401 gttttgcgag ttttactttt tccagacctg gaatagtctg agcagctttt tcctgaacgt
     5461 caatcggaag gcttgttgaa aaaccattca tataaatagt atttccgttg agagtttcag
     5521 gttcaagaaa tatctgatga cgcggcttat ctgaaaatct tgaaatttta tcttcaattg
     5581 acggacaata tctaggacca attcccttta tcctgcctgt aaacataggc gaatcttcaa
     5641 atcctgttct cagaatttca tgtgtttttt catttgtatg agtcaaatag cagtcaattt
     5701 gctcctgaag tggaaatttt aagtactttt tatgtgaaaa tggttttgga ttatcatcac
     5761 ccttctgaac ttcagtaact gaaaaatcaa tggtttcttt aaataatctt ggaggggttc
     5821 ctgtttttaa tctacccgtt tcaaaaccca ttccaagcat acattctgaa agacccgtag
     5881 ctgccttttc acccagtctc ccgccttcga tatttgactt tccggtatgc attacggcat
     5941 tgaggaaagt tcctgaagta attatcaccg aactacattt aatttccaga ccggtttcag
     6001 tttttattcc atttattata aaagaatatc cgttttcatt atttttcatt tcagaaacaa
     6061 tcaactcttt caccatatcc tgaataattt caatattttc ttggtcttta ataacttttt
     6121 tagcttcatt agaataaaga tctttgtcgg actgacatct tggcgaccag attgcaggac
     6181 cttttgaagt attgagcatt ttgaactgaa tccccgtctt atcagcaatt tctcccataa
     6241 ttccgccaag cgcatcaatt tctctgacaa gatgaccttt cgcagttcct cctattgcag
     6301 gattacaact cattctgccg atggaattca catccattgt aacaagtccg acagataatc
     6361 ccatcctcgc agctgcatat gaagcctcga ttccggcatg cccggcacca actactatta
     6421 tgtcaaaatt tttcataaat ttaattactc aatacggatt atttgaactg ccaatttatt
     6481 caattctaag ttaattttct atgcatagaa ttatattttt ttaagtatta tatatttata
     6541 tcaattgtta ttaattagta aaattttgta ttataaatgt gcaatttcaa attcaatttg
     6601 cgacacttga acccattcaa cttaagtatt tgattttaca cagtagttaa aatgtttctt
     6661 gagaaacaat tttatacaat ttattatcat agatttttat atttgttaga aatttgttaa
     6721 taagattgtt aagaagtaca agtactcatt tatgattgaa aaatgttgct tacagtaaaa
     6781 gtattattcg agctgctatt aatttggaga tctgatatag atcttaaatt cttgacctgg
     6841 gaatgttact ttctaaattg attttgaagg cttttttcaa aaataatttg atatattcta
     6901 tgactttgtt agaccgtgct cttcggtcga cgcaatctca attaattatc ctgtctactg
     6961 aattttttac tttcgaaatt tagatttctt cgcttcgatt actaaaacat tttatctgaa
     7021 ttcatttgct gttaatttct tatcaattac tttgggtgac cttgtttttc gttcgaagca
     7081 gtctcaaata attatccggg atggcatact gaatttcaaa ctttcgaaat ttagatttct
     7141 tcgttttact tgcaaaatca atttataaaa ttaatcttaa gtttgacata ttgtatgact
     7201 ttgcgagaac gtattctgag gtagaagcaa tctcaaaata atctgaaata catttctata
     7261 agattttctg tttagaatct tagatttctt ctcttcgctc gaaaatcaat ttctgattta
     7321 aatctgaaga ttaaagttta acttttagtt tgattttgcg agaccaagct ctccggtcga
     7381 cgcaatccaa taacttctcc aggttttagc ttttgtgttc ttaatttaga ttcgcagatt
     7441 gattcggctc gccccgcaaa atcactcaaa atctaatcat tttatatcat caaataatat
     7501 tactttacgt gaccgtgctc tttggtcgaa ggaatctctt aaaacttttc aaatgttcag
     7561 gctgttcgaa atggagag
//
LOCUS       DAORVQ010000165         7446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5903_length_7446_cov_5.119470, whole genome shotgun sequence.
ACCESSION   DAORVQ010000165 DAORVQ010000000
VERSION     DAORVQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5903_length_7446_cov_5.119470"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1733
                     /locus_tag="PLX80_11585"
     CDS             111..1733
                     /locus_tag="PLX80_11585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQY21476.1"
                     /translation="MHQTKYVFLTGGVVSSLGKGIAAASLGLLLKARGLKVTIQKLDP
                     YINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFLDENMSRANNMTTGQIYNEVIT
                     KERRGDYLGATVQVIPHITDEIKRRIRALESVKKYDVIISEIGGTVGDIESLPFLEAM
                     RQIMFDVGKQNSLSIHLTLVPFIKSAGELKTKPTQHSVKTLLEIGIQPDMLLCRSEMN
                     LSKELREKIGLFCNIEQGSVLQALDAKSIYEVPLNLKKEKFDEVVMKRLNLKSSEPNL
                     RNYKKVVNKILNPGKEITIGICGKYNIIPDAYKSILESFIHAGAYNEVKVNLKWINSE
                     ELEKGKANIGKILGNISGLLICPGFGKRGVEGKIKAIKFVREKKIPFLGICLGLQCAV
                     IEFARNVCGLRSANSSEFEKSKDSVIDIMEYQKSVKVMGGSMRLGSYPCIVEKNSLAY
                     KCYKKESINERHRHRYEVNNNYRKLLTENGLKLSGLSPDSTLVEMIELPQTKHPFFIA
                     SQFHPELKSRIINPHPLFREFVKACIIYSVKK"
     gene            complement(1742..2809)
                     /locus_tag="PLX80_11590"
     CDS             complement(1742..2809)
                     /locus_tag="PLX80_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQY21477.1"
                     /translation="MFQNYITSHDGKFFDGKDEFRFIGCNMYELANVESDISQKMLAS
                     AKDQRFDVVRFWVFNKTGRNNLNNICEISTELNLRLIPVLADNWGYLMNEEINDEWYL
                     SGYKNNYLQNILDLSFEFSDRKEILLWELINEPASVNFESVFNFTKDVSAEFKKINSN
                     HLLSVGTIGGIGDKFGNEFSRFNSSNFEKLYSLKNLDAVSIHDYSFNSTIAERLDIHQ
                     RFKGKYSSSAILNNLDRIINFIPGLIDKFTLRKFNDVYDIPLTLRSIWQKYISKNIQT
                     SAKLKKPLYIGEIGFKKEMGSDRKKVIEKELVKYLKAGVSGILLWSFESEGRSKDGHD
                     YGFDENDGFKEIIKKIKNEYL"
     gene            3133..4515
                     /locus_tag="PLX80_11595"
     CDS             3133..4515
                     /locus_tag="PLX80_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21478.1"
                     /translation="MKKFLKYSSIFLVLALIFIFIWFFRYRYYLLVSVDKIPEMELSS
                     IDGYTDKMFYFNNEKIKFYLRSTEDSSTAIITKIISPYQYVPLDTFYFDRSEQNINPD
                     QSESGCNWDLTHEMNLNSDYTQGYYNVILSNSIDTFNLTITIGDFDNNNEFVVFAPLA
                     TFAAYNPWGGKSLYHNYTDSSNVFFVSTQRPNTGVQYFKENYNHIDVNVCANIFNYFY
                     KNQNANILPDYYLEERPELLSKCKFAVLSYHCEYFSEKMYDKIEEMVFDQNIPLISLG
                     ANQIFWKVKWHDNFTRMECRKDLTFFGEGFDPGGMWRHNLRSESDLLGVRYTGKGLHT
                     FAPYKVLNSSHWLYSGLNISDGELFGIKGINSFPICGDETDKVPFWMEFSTETIAKGL
                     NTDIADTIKIYNKDDADWNSKGGGEIVLREIGNIAVLSTGSIYSGSGLGSDKVFTGII
                     DNFLNKYFIR"
     gene            4743..7061
                     /locus_tag="PLX80_11600"
     CDS             4743..7061
                     /locus_tag="PLX80_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQY21479.1"
                     /translation="MNFEGLNSEEVNKRISSGQVNTYDKNKSKTVKEIFIENIFSVFN
                     LVILSIIIFVLYFYFTTGDFRLILDSIGIMMIAVLNTFIAIYQEIKAKRALDKVKLLL
                     KKEVTVIRDGNEQSINPSEVVKDDVIKILRGDQIIVDGRVIFSNHLEIDESLLTGESN
                     PIHKKENEEVLSGSFCLSGNGFYKAEKVGNESFASKITSQAKKYKFVLTPLQRKIDAI
                     VKILFLTAVVLVILEIIFNTEGFTNDFVRKISTILISLVPQGLVLMASVTFAIGVYRI
                     SKLGAIIQKLNAIESFSNVQIVCMDKTGTLTQNKLSIKEITLLSDKHNRKEVEQLIGT
                     YANLTSDKNATIEAVNIFPPLENYSMEDEFPFSSERKFSMLKTISDSNKSTFILGAYD
                     VLINYMSEDDIKLTQGIFESKKIYIYRNLLFGKVDAEISFEDLKSGMNNIKIEPFCII
                     SITDKIRDDVFDAIKLFEKNDIQIKILSGDSAKAIQEVSNEIGWKIKDDDLITGIEME
                     SLNDKDFLKTVQEKVIFARLNPEHKLRIIRSLRNEKIYTAMIGDGVNDLPAIKEADLG
                     IAMEEGSSITKEIADIVLLNNKFSLMPAIFDEGNKIVNTVKSISKLFLTKNFFVIYIT
                     LLSVFFLLEFPLTPRRVSLINIFNIGLPAFIITLKNTNIEKTKNFLSDVISFVLLSAL
                     IIVAAGYTGIYFAEKYYSVSNMDLQMVMLTIIIITNFANYFAIMVKKNDQNNMTYLLY
                     GLAILFVYIFFAASTLHRNIYFHKIFLRDRLY"
     gene            7177..>7446
                     /locus_tag="PLX80_11605"
     CDS             7177..>7446
                     /locus_tag="PLX80_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21480.1"
                     /translation="MKFKFKLHTQIIIGLLLGAVFGSIFHVNQNKLKYESDGSEIISE
                     DWKDVKFISGDSVLNNFDAGTQLSIIKMFTVLKDKRKVKELKIEIN"
BASE COUNT         2550 a         1114 c         1359 g         2423 t
ORIGIN      
        1 gttttttaat tcagtatccg cagagtctga agagcacgtg actcttcggg aacatattgt
       61 ggataataat aaagtcggtt catttttaaa aaattaaaat ttaataagtt ttgcatcaga
      121 caaagtatgt gtttttaacc ggaggtgttg tttcctcact tggaaagggg attgccgcag
      181 cttcactggg attactgcta aaggcaagag gattaaaagt cacaattcaa aagctggatc
      241 cttacatcaa tgtagatccc ggtacaatgt cgcctactca gcacggggaa gtgtttgtga
      301 ctgaagacgg agcggaaact gatcttgacc tcgggcatta tgaaagattc cttgatgaaa
      361 atatgtctcg ggcaaataac atgacgaccg gtcagatcta taatgaagtg atcacaaaag
      421 aaagaagagg tgactatctc ggcgcaactg ttcaggtgat tccgcatatt actgatgaaa
      481 taaaaaggag gatccgagct ttagagtcag tgaagaaata tgatgtaata atttctgaga
      541 tcggcggaac agttggtgac attgagtctc tgccgttcct tgaagcgatg agacagataa
      601 tgtttgacgt gggaaagcaa aactcattaa gcattcatct gactttggta ccgttcataa
      661 aatctgccgg tgagttaaag acgaaaccga ctcagcattc agtaaagact ttactggaga
      721 tcggaataca gccggacatg cttttatgca gaagtgaaat gaatttgtca aaagagctta
      781 gggaaaagat cggactgttt tgtaatattg agcagggctc agttttgcag gcacttgatg
      841 cgaagtctat atatgaagtc cctttgaatc tgaagaagga aaaatttgat gaagtagtaa
      901 tgaaaagact taatctgaaa tcttcagaac caaatttaag aaattataaa aaggttgtaa
      961 ataaaattct taatcccggc aaggaaatta cgatcggaat atgcggcaaa tataatatta
     1021 ttcctgatgc ttataaaagt atattggagt catttatcca tgccggagct tataatgaag
     1081 tgaaggtaaa tcttaaatgg ataaattctg aagaacttga aaaagggaaa gctaatattg
     1141 gaaaaatact tggtaatatc agcggactgc ttatctgtcc gggatttgga aagaggggag
     1201 tcgaaggaaa gatcaaagca attaaatttg tcagagaaaa gaagatcccg tttcttggaa
     1261 tatgtcttgg actgcaatgt gctgtaattg agtttgcgag aaacgtctgc ggattgagat
     1321 cagctaattc atcggaattt gaaaagagta aagacagtgt aattgatata atggaatatc
     1381 aaaagtctgt aaaagtaatg ggaggttcga tgcggctcgg atcatatccg tgtatcgttg
     1441 agaaaaattc tttggcatat aaatgttata aaaaggaatc aataaatgaa agacacagac
     1501 atcgttacga agttaataat aattacagaa aattactgac tgaaaacgga ttaaaacttt
     1561 ctggattatc tccggacagt actttggtag agatgatcga actgccgcag acaaagcatc
     1621 cattttttat tgccagccag tttcatcctg aattgaaatc aagaataatt aatccacatc
     1681 cgcttttcag agagtttgta aaagcatgta taatttattc tgttaaaaaa taaatgttga
     1741 attataagta ttcattttta atctttttaa taatttcttt gaatccatca ttttcatcaa
     1801 atccataatc atgtccgtct ttagatcttc cttcggactc gaaagaccac agcaatattc
     1861 cgctgacacc tgctttcaga tatttaacta attccttttc aataactttc tttctgtctg
     1921 aacccatttc ttttttgaat ccaatctctc caatgtaaag cggctttttt aattttgctg
     1981 atgtctgaat atttttggaa atatactttt gccagatact tcttaatgtc agtggaatat
     2041 catagacatc attaaatttc ctgagagtaa atttgtcaat taaacccgga ataaaattta
     2101 ttatcctgtc cagattattt aaaattgccg aacttgaata tttccctttg aatctctgat
     2161 gaatatccag tcgctcagca attgtagaat tgaaactgta atcatgtata gagacagcat
     2221 cgagattttt tagagaatat aatttctcaa aattggatga attgaatctt gaaaattcat
     2281 ttccgaattt atctccaatt ccgcctatag tcccgacaga gagcagatga ttagaattaa
     2341 tttttttaaa ttcggctgat acatcttttg taaaattaaa tacactctcg aaattaactg
     2401 aagcaggttc atttattaat tcccataaaa gaatttcctt tcgatcggaa aattcaaatg
     2461 acaggtcaag tatattttgc agatagttat ttttatatcc ggataaatac cattcatcat
     2521 taatttcttc attcatcaga tatccccagt tgtcggcaag tacggggatc agacgaagat
     2581 taagttctgt actaatttca cagatgttat tgagattatt tcttcctgtt ttgttaaaca
     2641 cccagaatct tacaacatca aaccgctgat cttttgcaga tgccagcatc ttttgtgaaa
     2701 tatcagattc gacatttgcc aactcataca tattgcagcc gataaaacga aattcgtctt
     2761 ttccgtcaaa aaattttccg tcatgtgatg tgatataatt ctgaaacata ataattttgt
     2821 ttttaaaata tttaatctaa aactaacttt ctattgtaaa gagattgatg cagttattat
     2881 tgaaatttat ttgatccaca gaataatatt ctatgctacg caacacagaa tgctattcgg
     2941 tgcttcgatt cagacattca cttaatttga atcattgaac ggaatttatt atttcgttag
     3001 tttgtcaggc atgcattata tttttatata gtttaagaaa tttacaatgt gaattgataa
     3061 atagatttta ctgtaagtat ctatctatat aacggactga ctttgacgga gataaaataa
     3121 atcaaaatga taatgaaaaa atttcttaag tattcttcaa tctttttagt cttagcatta
     3181 atttttattt ttatctggtt tttcagatac agatattatt tgttggtatc agtagataaa
     3241 attcccgaga tggaactgtc atcaatcgat ggctatacgg ataaaatgtt ttactttaat
     3301 aatgaaaaga ttaaatttta tttaaggagt actgaagatt cttccactgc aattattact
     3361 aagatcattt caccatatca gtacgtaccg ctggatactt tttattttga caggtctgaa
     3421 caaaacatca accccgatca gtctgaatca ggctgtaact gggatctgac acatgaaatg
     3481 aatttgaatt cagattatac tcagggatat tataatgtta tactttccaa ttccattgat
     3541 acctttaatc tgacaattac cattggagat tttgataaca ataatgagtt cgtagtcttt
     3601 gctccgcttg cgacttttgc tgcgtataat ccctggggtg gaaaatcgct ttatcataat
     3661 tacacagatt cttccaacgt attctttgta tctactcaaa gaccaaatac tggagttcag
     3721 tattttaagg aaaattataa ccatattgat gtgaatgtat gtgcaaatat ttttaattat
     3781 ttttacaaaa atcaaaacgc aaacatactt cctgattatt atcttgagga aagacctgag
     3841 ttactttcaa aatgtaaatt tgctgtattg tcttatcact gtgaatattt ttctgaaaag
     3901 atgtatgata aaatagagga aatggttttt gatcagaata ttcctttgat ttctttaggt
     3961 gcaaatcaga tcttctggaa agtaaaatgg catgataatt ttaccagaat ggaatgcaga
     4021 aaagatctca cattttttgg agaagggttt gatccgggag gaatgtggcg gcataatctc
     4081 cgttcggaat cagatctctt aggtgtcaga tacaccggca aaggacttca cacatttgca
     4141 ccatataaag ttttgaattc atctcactgg ttatattcag gtttgaatat ttcagacggt
     4201 gaactcttcg gtattaaagg aattaacagt tttcctatat gcggtgatga aacagataaa
     4261 gtacctttct ggatggaatt tagtaccgag actattgcca aaggtctgaa tactgatatt
     4321 gcagatacga ttaaaatcta taataaagat gatgcagact ggaacagtaa aggaggtgga
     4381 gagatcgtgc tcagagagat cggaaatatt gcagtacttt ctacaggttc tatttacagc
     4441 ggatcgggtc ttggtagtga taaagttttt acagggatca ttgataattt tttgaataag
     4501 tattttatca gataaactga atttaatttt ttctaagaaa tatttgaaca tttaacagat
     4561 cacacaaaat tcaggatatt ttttttgcca cgaaacactg aaaattaatc attgtaatga
     4621 aacacagaat gttattctgc gctaccaata tttaaataag acttattccg atatattctt
     4681 taaaatcaga atttgataaa ttaattaaat ttgacacatt acattatcag catcatacat
     4741 tattgaattt cgaaggatta aattctgaag aagtaaataa acggatttct tcgggacagg
     4801 taaatacata cgataaaaat aaatctaaaa cggtaaaaga aatttttatt gaaaacatat
     4861 tctctgtttt caatttagtt attttatcga tcatcatatt tgttctatat ttctatttta
     4921 ccacaggtga tttcagactg atcctggaca gtataggtat aatgatgata gccgtgctga
     4981 atacttttat tgcgatctat caggagataa aagcgaagcg ggcactggac aaggtcaaac
     5041 tccttttgaa aaaagaagta actgttatca gagacgggaa tgaacagtca ataaatccat
     5101 cggaagtagt aaaagatgat gttataaaaa ttttaagagg tgatcagatc attgtcgatg
     5161 gcagggtcat tttctcaaac catctggaaa ttgatgaatc tctcctgacc ggagaatcaa
     5221 atcccattca caaaaaggaa aacgaagaag ttctctcagg aagtttttgt ctgtcgggta
     5281 acggatttta taaagctgaa aaagttggga atgaaagttt tgcatccaag atcacttcac
     5341 aggcaaagaa atataaattt gttctcaccc ctcttcagag aaagatagat gctattgtca
     5401 aaattctttt tttaactgca gtggttttgg taatactcga aattattttt aacactgaag
     5461 gatttacaaa tgattttgta aggaagatat ctactatact tatctcatta gtgccgcagg
     5521 gacttgtcct gatggcttct gttacttttg cgatcggcgt ttacaggatc agcaagctcg
     5581 gtgcgatcat acaaaagctt aatgcgatcg aatctttttc aaatgtgcag atcgtctgca
     5641 tggataagac cggtactctt actcaaaata aactgagtat aaaagagatc acattattat
     5701 cagataaaca taacaggaaa gaggtcgaac aacttatcgg tacttatgca aatcttacca
     5761 gtgataaaaa tgctacgata gaagctgtga atatttttcc gccattggaa aattattcta
     5821 tggaagatga attccctttc agttcggaaa gaaaattcag catgctgaaa actatttcag
     5881 acagtaataa gtcaacattt atacttggag catatgatgt attgataaat tatatgtcag
     5941 aagatgatat taaattgaca cagggaatat ttgaatcaaa gaaaatttat atttacagaa
     6001 atcttttgtt tgggaaagtc gatgctgaga tttcatttga agatctgaag agcggcatga
     6061 ataatataaa gatagaacca ttttgtatta tatcaatcac tgataaaata agagatgatg
     6121 tatttgatgc gataaaactt tttgaaaaaa acgatattca gataaagatt ctatcaggag
     6181 attcagctaa agcaatacag gaagtttcaa atgagatcgg atggaaaatt aaagatgatg
     6241 atctgataac cggtattgag atggaaagtc tgaatgacaa agatttttta aagacagtac
     6301 aggaaaaagt tatatttgcc agattaaatc ctgagcataa gctgaggata ataagatcat
     6361 tacgaaatga aaagatatat actgcgatga taggtgacgg agtaaatgat ctgccggcca
     6421 tcaaggaagc agatcttgga atcgcaatgg aagaggggag cagcattacc aaagagattg
     6481 cagacattgt acttttgaat aataaattct ctctgatgcc ggcaatattt gatgaaggta
     6541 ataaaatagt aaatactgtg aagtctatat caaaactttt tttaacaaag aatttttttg
     6601 taatatatat tacactgctt tctgtatttt ttctgctgga gtttcctttg actccgagaa
     6661 gagtttcgct gataaatata ttcaatatcg gactgcctgc atttatcatt accttaaaga
     6721 ataccaatat tgagaaaact aaaaactttc tttcggatgt tatttctttt gttttattgt
     6781 cagctctgat aatagtggct gcaggctata ccgggattta ctttgccgaa aaatattaca
     6841 gcgtttcgaa tatggatctt cagatggtaa tgctgacgat cattataata acaaacttcg
     6901 caaattattt tgcaatcatg gtgaaaaaaa atgatcaaaa taatatgacc tatttattgt
     6961 atggactggc tattttgttt gtatatatat tctttgcagc atcgacttta cacaggaata
     7021 tttactttca taaaatcttt ttacgagatc gattatacta atccggttct gtggccgctg
     7081 attttaacag taagtgtaac tgcctctgta atattgtttt taatgcaaaa gctgcgggaa
     7141 agaatattag aaaaattcac gaaataaaaa ttcacaatga aatttaaatt taagctgcac
     7201 actcagatca tcattggttt attacttgga gctgtctttg gatcaatctt ccatgtaaat
     7261 caaaacaaac ttaaatacga gtctgacgga agtgaaataa tttctgaaga ctggaaagat
     7321 gtaaagttta tcagtggtga ttctgtctta aataattttg atgcagggac acagctcagt
     7381 attattaaga tgtttacagt acttaaagat aaaaggaaag ttaaagagct gaagattgaa
     7441 atcaat
//
LOCUS       DAORVQ010000166         7380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_5995_length_7380_cov_6.201638, whole genome shotgun sequence.
ACCESSION   DAORVQ010000166 DAORVQ010000000
VERSION     DAORVQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_7380_cov_6.201638"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..1813)
                     /locus_tag="PLX80_11610"
     CDS             complement(335..1813)
                     /locus_tag="PLX80_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21481.1"
                     /translation="MKNSRLIKILKSFSRVELKEFERFLRSPFFNCPKFVLKLFLILK
                     KLYPDFKEKNIDGESLFKKLYGDRKFNGLLMRRMISDLIRFSEEYLRYKNFSQNESFR
                     NICLLNELSKRDLDSVLLLKYNSVVKKSDDAPLYDPDRLLQMFQVHTEMKNSRSKLRD
                     DLMNISYTRSIEAISVLYLRILFSYINHTNTFPDAGKNASYISLSFFKYFDRAGFFKE
                     TEILDDNYSSYLRLINYCLLITENKNRKDIYDKLKYLIMNYPERFSPFERISCLAVMQ
                     RFCNHQNLKNNNQYLMESFEIQKLILNEKLFLENSQFLQLSFCRNFVIKCLSLAKKDE
                     LLKFIRQYGIYFSPEYKDAFINYCKALLKFEEGNFDRSLFYISGCDLENAIFRKDLKI
                     LKIKIFYELNYLDSLFSETDSFRHFIKSNLTINPDIKTKAKNFIGNISALSKLKDFHS
                     AKDLNILYKKIENETKTSEKKWLLNKIIEINKKNGLKNFKPF"
     gene            2102..>4568
                     /locus_tag="PLX80_11615"
     CDS             2102..>4568
                     /locus_tag="PLX80_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HQY21482.1"
                     /translation="MKTRFSLLLIIMFIAVLTQAQNNREKESESESESETDALSLQSI
                     NGLIASQRFGASVSDCGDLNGDGYDDFISGAHLTNSNTGRAYIYFGGNNMSSSPSIVL
                     NGEASNNYFGISVANAGDLNADGYNDVIVGAYGYSSNAGRAYIYYGGFNMDNVADVVL
                     SAPVSSSFGYSVSTAGDVNGDGFSDVIVSGHTFSSNRGRAYIYYGGLIMDNSPDKTIE
                     GSATGGFFGFSLSGAGDVNGDGYSDVIAGAYAISAFTGSAYLYYGGANMDTISDLTFT
                     GEAASNFFGQSVSDAGDVNGDGYSDLLIGASLYSGSTGRAYIFYGSGSMDNIADVTFT
                     GELAGNNFGRGISGAGDVNGDGFSDILIGAQSYNGSRGRAYLYYGGVSMNNVSDMILN
                     GEASNNYFGFSVTGGGDINGDGYRDFIVGAYGVNSNKGKVYIYKNAMIGTDIPDVAVY
                     GENLSEMGKSVSDAGDLNGDGYDDYIIGASAYGDFATGRVYIFFGSVNPDTIPELIIN
                     GFANNSRFGTSVSGAGDVNADGYDDIIIGAPEYNSSQGRVYIYFGGSIMDTIHDVQMT
                     GATSLYRFGHSVSSAGDMNGDGFSDVIVGIPGTSSSRGSIRIYNGGNPMDSAFDQIYT
                     GTVADEQFGHSVSEAGDVNNDGFSDVIFGAPGYQGYKGVAVILLGNNIGGFSIRGDNT
                     GDQLGYSVSGAGDINGDGYSDVVIGAPYANGNLGKAYINLGSSFQDTSKDLEFTANYF
                     KFGNSVSGAGDVNNDGYDDFIICEEQYLGGAGRAFIYFGGYQPDNIHDINLNYNSQYE
                     RFGISASSGGDINSDGLDDIIIGA"
     assembly_gap    4569..4668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4669..5946
                     /locus_tag="PLX80_11620"
     CDS             <4669..5946
                     /locus_tag="PLX80_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HQY21483.1"
                     /translation="SSGGDINSDGLDDIIIGAFVNEINGSSAGAAYVYLSSAPSVKPM
                     LISVKDVPNDQGGKVNLKWTRSAYDVLGNDMITDYLIQRSLPPGGGGFYWENIAYIPA
                     TKESYYAYNDNTPFDSLPGNSGSLYYRITARTNNANNLWRSNIVYGRSIDNIAPLMVS
                     PFNASAVIDDVQLTWKRSSAPDLLNYILYRSYSPTINPNSETPYATTVDSAYVDTSPL
                     SGLFYYFIVAQDIHGNYSPVAVALSPNITLNLSMYIEGFYNSVNDQQVSDTITVELRN
                     SISPYTTADVSKAVVSSTGSAVLNFGGAADGNYFISVKHRNSIETWSANSNSFSRTSP
                     ANYDLSSSSSQAFGNNLIQIDSSPLRFGNYSGDVNQDGVIDLSDGSLIDNDAFNFASG
                     YLPTDVNGDDVTDLADAAVSDNNAFNFVGIVRP"
     gene            complement(6184..>7380)
                     /locus_tag="PLX80_11625"
     CDS             complement(6184..>7380)
                     /locus_tag="PLX80_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21484.1"
                     /translation="NEDSHKREYYLLNRLREKRSYKEFEKIFNTSTANKELPEKGGAL
                     DFINQFYSAQNSMVYCIENGDISGAVDSIISQGECFAVAALIKGYRNPELSIAAKTHN
                     VNFRENLIDNLMSHLDSPGVLEVMKKNNDKFYPYVAVNYAAYLMMKHTEDHKYYFEFK
                     KMVDEYYSLFGQSERFYLYAMLISYCSMQCRNDNPEIFGREQFEIHKRSVELGVYKNS
                     ENDIMDIVKFRNIAICAFTFNETDWFETFVNDFINELKPEYRENMRYFSYAYVHFGRK
                     EFGKSLENLIMVHDDFFYFKMDAKKLLLLIYFELGYFEQANSLIKSVKEYIANTKDLT
                     DKIKDRERIFIKVVNELLNLKESGNKKDIGILETEISKDLNLTFKIWLLEKVNELKGD
                     RSPSAG"
BASE COUNT         2265 a         1192 c         1422 g         2401 t
ORIGIN      
        1 gaacgagaga agtgtgtatt aatattttgc ttgttttcgt ttgcgttagt tcaaatccct
       61 cgttcccaat ctcctgattg ggaacggagc cggatctggt tagagaagat aggaccggat
      121 gtagaattca aaatgtgatt tactttataa cttagacttc cgtgaacttt ctaccggttt
      181 tcgtttacgt tcccaatcgg gagatgggga acgggagaaa catcaacatg ggcgaaattc
      241 ttttaattga tcatacatta cattcctgat aaagatcaat ttcaaatgtc aaataatttt
      301 agttaaatat tcggattttc atttatgtat aaaatcagaa cggtttaaaa ttttttaaac
      361 cgttcttttt atttatctca attattttat taagcagcca tttcttttcg cttgtctttg
      421 tttcgttttc gattttttta tataaaatat tcaggtcttt tgcagaatga aaatctttaa
      481 gttttgacaa agctgaaata ttaccgataa aattttttgc tttggtttta atatccggat
      541 tgatggtaag atttgattta ataaaatgtc tgaaagaatc cgtttcactg aataatgaat
      601 ccagataatt aagctcataa aaaattttaa ttttgagaat cttcagatct tttctgaaaa
      661 ttgcattttc aagatcacat cctgagatat aaaaaagtga tctgtcaaaa ttcccttctt
      721 caaactttaa taaagcttta cagtaattta tgaatgcgtc tttgtattcc ggactaaagt
      781 atatgccata ttgtctaatg aattttaata actcgtcttt ctttgcaaga cttaaacatt
      841 tgattacaaa atttctgcag aagctgagct ggagaaactg agaattttcg agaaataatt
      901 tttcatttag aattaacttc tgaatttcaa aactttccat taaatattga ttgttgtttt
      961 ttaaattctg atgattacaa aaccgctgca ttacagccag acagctgatc ctttcaaagg
     1021 gtgaaaatct ttccggataa ttcattataa gatattttaa tttgtcgtat atatctttgc
     1081 ggtttttgtt ttctgtgatc aaaagacagt agtttattaa tctgagatat gatgaataat
     1141 tatcatcaag aatttccgtc tctttgaaaa aaccggctct gtcaaagtat ttaaaaaatg
     1201 aaagagaaat gtaagatgca tttttaccgg catcaggaaa tgtatttgta tggtttatat
     1261 aagaaaataa aatcctcaga tatagtacag atattgcttc aattgatctt gtataactta
     1321 tattcataag atcatctcta agttttgatc tggaattttt catttcagta tgcacctgaa
     1381 acatctgaag cagacggtca gggtcatata aaggagcgtc atctgatttt tttacaacgc
     1441 tgttatattt taaaagtaat acagagtcaa gatctctttt tgaaagctca ttcaacagac
     1501 agatattccg gaaggattca ttctgactga aatttttgta tctcaaatat tcttcagaaa
     1561 atctgataag atcggatatc attcttctca ttaacaaacc gttaaatttc cggtctccgt
     1621 aaagtttttt aaaaagactt tcgccgtcaa tattcttttc tttaaaatca gggtaaagtt
     1681 ttttaagtat cagaaacagc tttaatacaa atttaggaca attgaaaaac ggcgatctta
     1741 aaaatctttc aaactctttt aattccaccc gtgaaaaact tttaaggatt tttatcaatc
     1801 ttgaattctt cattttacgg taactttaaa ttattgtaaa aaggtgaaaa tttataaaaa
     1861 aagtcaatat ttataaatat atcattcatt taattaatta atccggtaac taatatgata
     1921 aatattcttt ggacaaaaaa ttcattaatc ttaatttgat ttaagtttct tcgggagaga
     1981 atcggatcac caaaaaattt ataatttaag cgaaactttt acattccaga taaatctctc
     2041 ccgaaatatc taatgaaagg tttcgcttgc tttttttaaa aatcagctga aaggagttat
     2101 tatgaaaaca agatttagtt tacttctgat cataatgttt atagccgtac tgacgcaggc
     2161 gcaaaataac agagaaaaag aatctgaatc tgaatctgaa tcggaaacag acgctttaag
     2221 tctgcagtcc attaacggac tgatcgcatc tcagagattc ggagcatcag tctctgactg
     2281 cggtgatctg aatggtgacg gatatgatga ctttatttcc ggtgctcatc ttacgaattc
     2341 aaatacgggc agagcttata tttattttgg aggaaataat atgagttcat ctccttcgat
     2401 agttcttaac ggagaggcgt caaataatta ttttggaata tcggtcgcaa atgcaggtga
     2461 tctaaatgct gacggctaca atgatgtcat agtcggagct tacggatatt catcaaatgc
     2521 cggcagagcg tatatatatt acggcggatt caatatggat aatgttgctg atgttgtgct
     2581 ctctgcacct gtatcatcga gttttggata ttcagtatcg acagcaggcg atgtaaatgg
     2641 cgatgggttt tctgatgtaa ttgtaagcgg tcatacattt tcatcaaaca ggggaagagc
     2701 atatatttat tacggaggat tgataatgga caattcacct gataagacga ttgaaggatc
     2761 tgccaccggg ggatttttcg gattttcgtt atccggtgcc ggtgatgtaa acggggacgg
     2821 atattctgat gtaattgccg gagcttatgc gatatcagca tttacgggaa gcgcatacct
     2881 ttattatgga ggagcaaata tggatacaat ttcagactta acttttaccg gtgaagcagc
     2941 ttcaaatttt ttcggtcagt cagtatctga tgccggcgat gtaaacggag acggatattc
     3001 tgatctgctg atcggagcat ctttgtattc cggatcgaca ggacgggctt atatttttta
     3061 tggaagcggt tcgatggata atattgctga cgtaactttt acaggggagc ttgcgggaaa
     3121 taatttcgga aggggaatct caggagccgg ggatgtgaac ggcgatggat tttctgatat
     3181 acttatcgga gctcagtcat ataatggcag cagaggcaga gcatatttat attacggcgg
     3241 agtaagtatg aataatgtca gtgatatgat tttaaacgga gaagcttcaa ataattattt
     3301 cggattttct gtaacaggcg gcggagatat aaacggtgac ggatacagag attttattgt
     3361 aggagcctac ggagtgaatt cgaataaagg aaaagtttat atttacaaaa atgccatgat
     3421 aggtactgat attcctgatg tagcagttta cggagaaaat ttgagtgaaa tgggaaaatc
     3481 tgtatccgat gccggtgatc tcaacggtga cggatacgat gattatatta taggcgcaag
     3541 cgcttacgga gattttgcca ccggaagagt atatatattt ttcggatctg taaatcctga
     3601 tacaatcccg gaactgataa taaatggatt tgcaaataac agccggtttg gcacttcggt
     3661 ttcaggagca ggtgatgtta acgcagacgg atatgatgat ataattatag gagctcctga
     3721 atataacagc agtcagggaa gagtttatat ttattttgga ggatcaatta tggacacgat
     3781 tcatgatgtc cagatgacag gagcaacaag tctttatcga ttcggacatt cggtttcatc
     3841 agccggcgat atgaacggag acggattttc ggatgtgata gttgggattc cgggcacttc
     3901 atcaagcaga ggcagcatta gaatttataa tggcggaaat ccaatggatt cagcttttga
     3961 tcagatttac acaggtacag ttgctgatga acaatttggt cattcagtgt ctgaagcagg
     4021 tgatgtcaat aatgacggat tttctgatgt aattttcgga gctcccggtt atcaaggata
     4081 taaaggagta gcagtaattt tactcgggaa caatattggt ggattctcaa tacgcggaga
     4141 caatacagga gatcaattag gttatagtgt atcaggagcc ggtgatataa acggagacgg
     4201 ttattcagat gtcgtgattg gagctcccta tgcaaatgga aatttaggaa aagcctatat
     4261 caatttagga agcagctttc aggatacttc aaaagatctt gaattcactg caaattattt
     4321 taagtttgga aattccgtat ccggcgccgg tgatgtaaac aatgacggat atgatgattt
     4381 cataatttgt gaagagcaat atttaggtgg tgccggcaga gcatttattt atttcggagg
     4441 ataccagcct gataatattc atgacattaa tctgaattac aatagtcagt atgaaagatt
     4501 tggaatttcc gcaagttccg ggggtgatat caacagcgat ggacttgatg acatcattat
     4561 cggtgcatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ttccgggggt
     4681 gatatcaaca gcgatggact tgatgacatc attatcggtg catttgtaaa cgaaataaac
     4741 ggatcaagtg caggcgctgc atatgtttat ctttcttctg caccctctgt aaagccaatg
     4801 ttaatcagcg tcaaggacgt gcctaatgat caggggggta aagtaaatct taaatggacg
     4861 agaagcgctt atgatgtttt gggaaatgat atgatcactg attatctgat ccagagaagt
     4921 ctgcctccgg gaggcggagg tttttactgg gaaaatattg cttacatacc ggctacgaaa
     4981 gaatcttatt atgcttataa tgacaacacg ccgtttgatt cattgccggg aaatagcggc
     5041 agtctatatt acagaattac cgcaaggaca aataatgcaa acaacttatg gagatcgaat
     5101 atagtttacg gaagaagtat tgacaacatc gctcctttaa tggtctcgcc gtttaatgca
     5161 tctgccgtta tagatgatgt gcagcttact tggaagagaa gttcagcgcc ggatcttttg
     5221 aattatattc tgtacagaag ttattcacca accataaacc ctaattcaga aactccttat
     5281 gccacgactg ttgattctgc gtatgttgat acatcgcctt tgagcggact gttttattat
     5341 ttcatagtcg cgcaggacat tcacggaaat tacagtcccg ttgcagttgc tcttagtccg
     5401 aatataacat tgaatctaag tatgtacatc gaaggatttt ataacagtgt aaatgatcag
     5461 caggtgagtg atacgattac tgtcgagctt agaaattcaa tttcacctta tacaacagca
     5521 gatgtatcaa aagctgtagt atcatcaaca ggcagcgcgg tattaaattt cggcggcgct
     5581 gctgacggaa attattttat atccgtaaag cacagaaaca gcattgagac ctggagtgca
     5641 aattcaaatt cattctctag aacttctccg gcaaattatg atctgtcatc ttcttcatcc
     5701 caggcattcg gaaataattt gattcaaata gattcatcac ctttaagatt cggaaattac
     5761 tcaggcgatg taaatcagga cggagttata gatctgagtg acggcagtct tatagacaat
     5821 gacgcgttca actttgcatc ggggtatctt ccgacggatg tgaacggaga tgatgtaaca
     5881 gatcttgctg atgctgcggt ttcagataat aatgcgttta attttgtggg catagtgcga
     5941 ccgtaataaa gtataatttc atcaaaatga ctggaagtaa gtaagttaca tttcacaatc
     6001 gagaaagatt aaccggcagc ctttggatca ccactgagat cctgagtgat ccaacaggtt
     6061 gtcagttcaa tctgacatct ggtcttaaat taaaatttta gaatttgata atttttgcag
     6121 cacatttcta tgaaaaatat tgatcgaata ttcatatgtt attttaagat cgttttagga
     6181 atgtcaaccc gcactgggtg accgatcgcc ttttaattca ttcacctttt ccagaagcca
     6241 tatcttaaaa gtaagattaa ggtcttttga tatttctgtt tcaagaatgc cgatatcttt
     6301 tttgtttccg gattctttta aattcaataa ttcatttact actttaataa aaattctttc
     6361 acggtctttt attttatccg taagatcttt tgtatttgca atgtattcct ttacggattt
     6421 tatgagagaa tttgcctgct caaaataacc gagttcaaaa tatataagaa gcagcagttt
     6481 ctttgcatcc attttaaaat agaagaaatc atcatgaacc attataagat tttccaggga
     6541 tttaccgaat tcttttcttc cgaaatgaac atatgcatat gaaaaatacc gcatgttttc
     6601 acggtattcc ggctttaatt cgtttataaa atcattgacg aaagtttcga accagtctgt
     6661 ttcattgaat gtaaatgcgc agatagcaat attcctgaat ttaactatat ccataatatc
     6721 attctcactg tttttataca caccaagctc aacagatctt ttatggattt cgaattgctc
     6781 tcttccgaat atttcaggat tgtcattccg acattgcata gaacagtatg agataagcat
     6841 tgcatataaa taaaatcttt cactctgacc aaaaagagaa tagtattcat ctaccatttt
     6901 tttaaattca aaatagtatt tatgatcttc tgtatgtttc atcatcaggt atgccgcata
     6961 gtttaccgca acatagggat aaaatttatc attatttttt ttcattactt ccaaaactcc
     7021 cggtgaatca agatgactca tgagattatc aattagattt tctctgaaat taacattatg
     7081 cgtctttgcc gcgatgctca attcaggatt tcggtaacct tttattagtg ctgccacggc
     7141 aaaacattca ccctgtgata tgatagaatc aactgctcct gaaatatctc cattttcaat
     7201 acagtacacc atagagtttt gagctgaata aaattgatta ataaaatcaa gggcacctcc
     7261 tttttccggg agttctttgt tggcagtgga agtattaaat attttctcaa attctttgta
     7321 agatcgcttt tctctgagcc tgttcaagag atagtattct ctcttgtgcg aatcttcatt
//
LOCUS       DAORVQ010000167         7279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6146_length_7279_cov_6.915421, whole genome shotgun sequence.
ACCESSION   DAORVQ010000167 DAORVQ010000000
VERSION     DAORVQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6146_length_7279_cov_6.915421"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLX80_11630"
     CDS             <1..731
                     /locus_tag="PLX80_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21485.1"
                     /translation="YDELTNDYFYFEARKIFKNLSVNFSDDYKLKFYMIFIYYCTRRQ
                     NLGDKKFHLELFQLYNEKLDLGFTSDFTEMIYPVNNFRDYVFIGIELGKTDWVRNFVK
                     KYSHLLPKKVREHEINLANSKLEFDIGNYENSLELVLKVKPNNYMHYLDSSSLKLVNY
                     YELNNIEEAYFEIDKLKHYLSNHNEIPKVHKRYNKNFIKIYNKLLKIKMDPKKKDIIV
                     IEGELKNMSFISRREWLQTKISDL"
     gene            complement(838..3978)
                     /locus_tag="PLX80_11635"
     CDS             complement(838..3978)
                     /locus_tag="PLX80_11635"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HQY21486.1"
                     /translation="MKKLILILPFILIGYFIFTGQKSGDDIKKWNNPHSTRVQITGEY
                     STLPMDNNYKFTTQPRILNTTKETYTIFPNRQVHPSFNTQSEVPITRHPTNHNIMFAS
                     ANTFGGGTAFSTGVYVTTDGGVSWFGSDTINNGSLNYGDPGIAIDGDGRFLMSFISLS
                     GRIANSVSSNNGLNWSTEIPIPGSTAQSDKNLSGVDDVSTSPYYGRFYTVFTEFQGPN
                     IDHIVISYSSDHGISWSSEAPVSPGPYFQHFHQGADVMVGPQGQVYVVWANNLQTAVE
                     DSLGFAKSTDGGVTWAAARNNASNMEGIRNTGQTFLNGIRANGFPRIAVDKTNGLRRG
                     WIYVTTSEKFLAPATDVSDIVLHRSTDEGVTWTKVRVNQDAPGTNKYQYMAAVNVDDN
                     GGLNVIYYDTRNTPTNDSAQIYISRSLDGGNTWTDILVSDHKFKPKAIQGTAGGYQGD
                     YIGITSGNNAIWPYWCEDITGLYQAWTARVDITAFPLNAFNINTPAANTTLIGYPNSQ
                     TPITVSWDTSASTASYKWIFGNPTANIRKITIPTLQNNFTITSEQLNNYLIGLGVAVG
                     DSLVGQWDVWAFRNNLANDSLKAANGPRTLTLKRGIPPLNTFNLNTPASGYSVLTSPN
                     DNSNILFNWSTSGPGVTYKWKFGSPAISATKIILSSDISGVDSTLTVPNYLMDGYLAS
                     IGVTAGDTISGQWSVYAYNGFDSVMAGQSNNITFIRQPKGDVLVLYDSTIANGRVSLD
                     SVTTNLARLDVTYDIYNKKGNTSTSAISFREYKQVILLGEGSSVMSNVTKDSLKSYLN
                     AGTMQTKSKLIIMADDIGYMIDRPASAFYDSAFARSMLGYQYVADRPGVGGKGIIGIT
                     ININLKDSTAGPSPDVIKRSSSLPISQTFNLYRYTLFADSMNAVGNISPTYNVAVMAL
                     DAESLRPVAENPNPFTVKRVLYGLMSFVDQLPTSNEPGSVNNVIPFEYKLSQNFPNPF
                     NPSTRISYSLPVSKLVTLKIYNVLGKEVRTLVNSKQDAGNYEVVLNAADMSSGIYFYT
                     ITAGDFVETRRMVLLK"
     gene            complement(4335..7073)
                     /locus_tag="PLX80_11640"
     CDS             complement(4335..7073)
                     /locus_tag="PLX80_11640"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21487.1"
                     /translation="MKTLLILIFLLIPFYSHSQDLQIPNNPYDAADEVTKSRNAFQRD
                     KWFYEQRMYPNNYIPADAYEKALNQKEEMKSQYGFSMTGIFDSWQNIGPKTGFYFSYS
                     NITSRMVTVKYHPYDPNIIYTGTAFGGVWKTINGGNNWSAMSDNEVSLASGTIAIDPD
                     DPNIIYYGTGEATYSAASYYGRGLLKSTDGGVTWTNYTSGLGSFSLFSKIVIRPGHSN
                     ELIATMGLKSNVGVSGGIYRSTNAGVSWTLLNSGRADDVVFSPTGDTAYAVGSGIGYR
                     ISTNGGVSFNSSGAISMGTRNHIAVCRTSPNIIYHARHQSPSIFVYKSTDAGLTFSQI
                     AVGQNFNGGQAWYDFYMHVNPHNPDYAYVGAVDIWRTTNGGATNFTNITNGYSGGGVH
                     VDQHNVDFHPIDPAQMVCINDGGIWKSTDYGSNWTNMNTDQTLTQFYRIASDPSNANH
                     ILGGTQDNGTQRTTGSINWTGAYGGDGGEVCFHSQNNNYILGETQNNGVFRSTNGGSS
                     FSSATSGLSGSGSWVGPLLSHPSTSGIFYTARQSVFRSTNWGASWSSISSGTSGTIRE
                     MAISSGTGNVIYASSGGTLYRSTNNGVNFSNASSGLSGNTITGIEVHPDSSNVAVITN
                     SGFGSGKVYKTTNTGASWINITGNLPDSPVNTVVIYHPGYPTSVYFIGSDVGVFVTHN
                     YGQTWQELANGLPNTVAMHLDHHKATNKLRIGTHGRGVFELQLSNFIFADIKVIPEGY
                     FNAGLNKLNYTDTVSAYIRNSSSPYNLIDSSNAVINDITFSGNFAFNNVASGNYYIVM
                     KHRNSIETWSKAGGESVTQGNAFSYDFTNSQAMAFGNNMVQVDNFPVNKYGLYSGDTD
                     NDGNIDIVDLGSIDNDISNFLTGYVQTDLNGDGTVDLADITFADNNTFNFVGIVRP"
BASE COUNT         2347 a         1299 c         1211 g         2422 t
ORIGIN      
        1 catatgatga gcttaccaat gattattttt atttcgaagc aagaaaaata tttaagaacc
       61 tgagtgtgaa tttcagtgat gattacaaac tgaagtttta tatgatattt atttattact
      121 gtacaaggag acagaattta ggtgataaaa aatttcatct ggagttgttt cagctttata
      181 atgagaaact ggacctggga ttcacatcgg attttacgga aatgatatat cctgtaaata
      241 atttcaggga ttatgttttt ataggaattg agcttggaaa aacagactgg gtcagaaatt
      301 tcgtaaaaaa atattcgcac ttgcttccta aaaaagtaag agagcatgaa ataaatttag
      361 ctaattcaaa actggaattt gatattggga attatgaaaa ttctctggaa ttagttttga
      421 aagtaaaacc aaataattat atgcactatc tggattcatc ttcactaaaa cttgtaaatt
      481 attatgaact taacaatatt gaagaagcgt attttgaaat tgataaatta aaacactatc
      541 tcagcaatca taatgaaata cctaaagtcc ataaaagata caataaaaat tttataaaaa
      601 tttataataa gcttttaaaa ataaaaatgg acccaaaaaa gaaagatatt attgtaatcg
      661 aaggtgaatt aaaaaacatg tcttttattt ccagaagaga atggttacaa acaaaaattt
      721 ctgatctata gaaataaaaa aaggcagtcc caaattctgc ctttttatct tccaatttca
      781 gaccgattta tatttccggt ctgaaaaatg aaaggaaatg aaatgaaaaa aattatttta
      841 ttttagtaac accattctcc tggtttcaac aaaatcacct gctgtgatcg tatagaaata
      901 aatcccactg gacatatcag ctgcattcaa tactacttca taattgcccg catcctgttt
      961 tgaatttaca agtgtcctta cttcttttcc aagcacatta tagatcttaa gagttactaa
     1021 ttttgatacc ggtaaactgt aactgatcct ggtagaagga ttaaaaggat ttggaaagtt
     1081 ctgagaaagt ttatattcaa aaggtattac attgtttaca gatccgggtt catttgatgt
     1141 cggtaactga tctacaaaac tcattagacc atataagact ctcttaaccg taaaaggatt
     1201 tggattttcc gcaactggtc tgagagattc ggcatcaagt gccattacag caacattata
     1261 agtcggtgaa atatttccga cagcattcat agaatcagca aataaagtat atctgtaaag
     1321 attgaatgtc tgactaattg gtaatgaaga agatcgttta atgacatccg gtgatggacc
     1381 tgctgtactg tcttttagat ttatattgat tgtaatacca attattcctt ttccacctac
     1441 acccggtctg tcagcaacat actgatatcc aagcatactt cttgcaaaag cagaatcata
     1501 aaatgccgaa gcgggtctgt cgatcatata accaatgtca tctgccataa taatcaattt
     1561 ggattttgtc tgcatagtac cggcattaag atatgacttc agggaatcct tcgtaacatt
     1621 tgacatcaca gaagatcctt cgccaagtaa tataacctgt ttatattctc tgaatgaaat
     1681 tgcactggta gatgtatttc cttttttatt ataaatatca tatgttacat caagtcgggc
     1741 aagattagtc gtaacggaat caagtgatac gcggccattt gcaattgttg aatcatataa
     1801 aacaagaacg tcaccttttg gctgccttat gaaagtaatg ttgttgcttt gtcctgccat
     1861 tacagaatca aaaccattat atgcatatac agaccattga cctgatattg tatcaccggc
     1921 agttactcct atactggcta aatatccatc cattaaatag tttggaactg tcaaagtgga
     1981 atctacacca gatatatctg aactaagaat tatttttgtt gctgaaattg cgggcgaacc
     2041 gaatttccat ttatatgtta cacccggtcc tgaagttgac cagttaaata atatatttga
     2101 gttatcattt ggagatgtta atactgaata tccgcttgca ggagtattta agttaaatgt
     2161 attcaaagga ggaatacctc tttttaatgt caaagttctt ggtccattag cagcttttag
     2221 tgagtcattg gcaagattgt ttctgaatgc ccaaacatcc cattgtccta caagagaatc
     2281 tcctacagct acacccagtc cgatcagata attattaagc tgttcagaag ttattgtaaa
     2341 attattttgt aatgttggaa tagtgatctt ccttatattt gcagtagggt ttccaaagat
     2401 ccatttatat gatgcagtag atgctgatgt atcccaactt actgtaatag gtgtctgtga
     2461 attcggataa ccaatcagtg tagtatttgc agccggagta tttatattaa atgcattcaa
     2521 aggaaatgca gttatatcca ctcttgcagt ccatgcctga tataaaccag ttatatcttc
     2581 acaccagtat ggccaaatag cattattgcc tgaagtaatt ccaatgtaat ctccctgata
     2641 tccaccggct gttccttgta ttgctttagg tttaaattta tgatctgaaa caagaatgtc
     2701 tgtccaggta tttccaccgt ccagagatct ggaaatatag atctgtgcag aatcattagt
     2761 tggagtattt cttgtatcgt aataaattac attaagacct ccgttatcat ctacgtttac
     2821 tgctgccata tactgatatt tatttgtacc tggtgcatcc tgatttactc tgacttttgt
     2881 ccaggttaca ccttcgtcag tagatctgtg aagtacaatg tctgatacgt ctgtggcagg
     2941 tgcaagaaat ttttcagaag ttgtaacata tatccaacct cttctcaaac cattagtctt
     3001 gtctactgct attcttggaa aaccatttgc gcggattccg tttaaaaaag tctgtcctgt
     3061 atttctgata ccttccatat tactggcgtt atttctagct gcagcccatg ttacaccgcc
     3121 gtcagtagat ttcgcaaatc ccaatgaatc ttctactgct gtttgtaaat tgtttgccca
     3181 tactacataa acctgacctt gaggtcctac cattacatca gccccctgat ggaaatgctg
     3241 aaagtatggt cccggactta caggagcttc acttgaccag gatattccat gatctgagga
     3301 ataagaaatt acaatatgat caatgttagg tccttgaaat tcagtaaaga cagtataaaa
     3361 tcttccataa taaggactag tcgaaacatc atctacacct gataaatttt tatcagattg
     3421 tgctgtggat ccgggaattg gaatttcagt agaccagttt agtccgttat ttgaagagac
     3481 agaatttgca attcttcctg ataatgaaat aaaagacatc aaaaatcttc catctccgtc
     3541 aatggcaata cccggatcac cataatttaa tgatccgtta ttaattgtat cactgccaaa
     3601 ccatgaaaca ccaccatcag ttgtaacata aacacctgtg ctgaatgctg tacctcctcc
     3661 aaaagtattt gcagatgcaa acattatatt atgatttgtc ggatgtctgg taataggaac
     3721 ttctgactgt gtattaaaac tcggatgcac ctgtctgttt ggaaaaattg tataagtttc
     3781 tttggttgta ttcagtattc ttggttgagt agtgaattta taattattgt ccattggtaa
     3841 agttgaatac tcacctgtta tctgaactct ggtggaatgt ggattattcc atttcttaat
     3901 atcatctccg gacttctgac cggtgaagat aaaatatccg ataaggataa atggtaaaat
     3961 taaaattagt tttttcattt ctgttgtagt ttagttagtt gttttgtaaa attagactgt
     4021 aaagttttac agcaattaaa atgttattat ttaattccat aaaaatataa aaacgatctt
     4081 aatttttcaa agaaaggtat tgatgattat acccaaatat ttaaaattga agtattgaat
     4141 tttaactatt tatacaataa tatttaggta aatgcagctg aataataccc taataggacg
     4201 aaatatcaga tattaaattt taaataataa atagttagta tattaaaata aaaaatgccc
     4261 gatttaataa tataaaacgg acattttttt aaaaaaaatt gaactctaat atttcatttg
     4321 tgaaatttta atatttaagg tctgacaatt ccaacaaaat taaaagtgtt attatctgca
     4381 aaagttatat ctgcaagatc aactgttcca tctccattca aatctgtctg tacatatccg
     4441 gttaagaaat ttgatatatc attatcaata gatccaagat caacaatatc aatattaccg
     4501 tcattgtcag tatcacctga atataaacca tatttattca caggaaaatt atctacctgt
     4561 accatattat ttccaaatgc cattgcttga gaatttgtaa aatcataact gaatgcatta
     4621 ccctgagtga cagattcacc acctgcttta ctccaggtct caattgaatt tctgtgtttc
     4681 attacaatat aataatttcc ggaagctaca ttattgaatg caaaatttcc gctgaatgta
     4741 atgtcattta taactgcatt tgaggaatca attaaattat aaggtgacga agaatttctt
     4801 atgtatgctg atactgtatc tgtataattt aatttattta aacctgcatt aaaatatccc
     4861 tcaggtataa ctttgatatc agcaaaaata aaattactca actgtaattc aaagacacct
     4921 ctgccatgag taccaattct taatttgttt gtagctttgt gatgatcaag atgcatcgct
     4981 actgtatttg gaagtccgtt tgcaagctcc tgccaggtct gtccataatt atgtgtaaca
     5041 aatactccaa catccgatcc aatgaaatat acacttgtcg gatatcccgg atgataaatt
     5101 acaactgtgt taacagggga gtcaggaaga tttcctgtaa tatttatcca tgaagcaccg
     5161 gtgtttgtag ttttataaac cttacctgaa ccaaatcctg aattagtaat tactgccaca
     5221 tttgaagaat caggatgtac ttcaattcct gtaattgtat ttcctgataa acctgaagaa
     5281 gcattggaaa aattcactcc attattagta gatctgtata gtgttcctcc cgatgaagca
     5341 tatattacat ttcctgttcc gctgctgata gccatttccc tgattgttcc gcttgttccg
     5401 gaagagattg atgaccagct tgctccccag tttgtagatc tgaatacact ttgtcttgca
     5461 gtataaaaaa ttcctgatgt acttggatgt gaaagtaacg gacctaccca gctccctgaa
     5521 ccagaaagtc ctgaagtcgc tgaactaaaa ctcgacccgc cgtttgtaga tctgaatact
     5581 ccgttattct gtgtttctcc taatatataa ttattattct gtgaatgaaa acaaacttca
     5641 ccaccgtcac cgccgtaagc tcctgtccag tttattgaac ctgtagttct ctgtgttccg
     5701 ttatcctgag taccgcctaa aatgtgattt gcattggaag gatccgatgc aattctgtaa
     5761 aattgagtaa gagtctgatc agtgttcatg tttgtccagt tcgaaccgta gtcagttgac
     5821 ttccatatac cgccgtcatt tatacaaacc atctgagcag gatctatcgg atgaaaatca
     5881 acattatgct gatcgacatg tactccgcct ccgctgtatc cgtttgtgat gttggtaaag
     5941 tttgtagcac cgccgttagt tgtcctccat atatccacag ctccgacata agcataatca
     6001 ggattgtgcg gatttacgtg catataaaaa tcataccatg cctgtccgcc gttgaagttc
     6061 tgaccaacag caatctggga aaaagttaat cctgcatcag ttgatttata aacaaaaatc
     6121 gaaggagatt gatgtctggc atgatatata atatttggtg aagtcctgca aacggcaata
     6181 tgatttcgtg tacccataga gatcgctccg cttgaattaa atgaaacacc tccgtttgta
     6241 gaaattcggt atccgatccc gctgccgact gcatatgctg tatctcctgt cggagaaaat
     6301 actacatcat cagctctgcc tgaatttaaa agtgtccagc ttacacccgc atttgtactt
     6361 ctgtaaattc cgcccgatac tccgacattg gattttaaac ccatcgtcgc aattaattca
     6421 ttactgtgtc cgggacgaat cactatttta gaaaacaaac tgaaagatcc gagtcctgaa
     6481 gtataatttg tccatgtaac tccgccgtct gttgatttca aaagtcctct gccgtaatat
     6541 gaagctgcac tgtatgtggc ttcaccggta ccgtaatata taatattcgg atcatcaggg
     6601 tcaatcgcta ttgtaccgga agcaagagaa acttcattgt cactcattgc tgaccagtta
     6661 ttaccaccgt taattgtttt ccatactccg ccgaatgctg tacctgtgta tataatattc
     6721 ggatcatatg gatgatattt tactgtaacc attcgtgaag taatattaga atagctgaaa
     6781 tagaatcctg tcttaggacc gatattctgc catgaatcaa aaatacctgt cattgaaaat
     6841 ccatattgag atttcatttc ttctttttga tttaatgctt tttcatatgc gtcggcaggt
     6901 atataattat tgggatacat tctctgttca taaaaccatt tgtctcgctg aaaggcattt
     6961 ctgctttttg taacttcatc tgcagcatca taaggattat ttggtatctg aagatcttgt
     7021 gagtgactgt agaaagggat taagagaaaa ataagaatta ataaagtttt cattttctta
     7081 tatttgattt taatttaaaa gtagaatttt gattattagc aaataacaaa tttaatctta
     7141 aatttatatt taaaaataat tctgatttta tataatggtt ttatcaatat ttaattaaat
     7201 ccagcagaga attttccttc tcaagacaac aatatgattt tgagggcgaa tagaagctat
     7261 caccgaatac atgaaaaga
//
LOCUS       DAORVQ010000168         7270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6168_length_7270_cov_5.828690, whole genome shotgun sequence.
ACCESSION   DAORVQ010000168 DAORVQ010000000
VERSION     DAORVQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6168_length_7270_cov_5.828690"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PLX80_11645"
     CDS             complement(<1..1245)
                     /locus_tag="PLX80_11645"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21488.1"
                     /translation="MKKFFLFIFMLASTSAFSQWTFISRIPGNPSVNSISAADQNVIW
                     VSCNGGVLYKTVNGGVNWMLRNSGLPVTGLIGISALDTSSCWVGTETGSIYHTSNGGM
                     NWTVQFSLAGSFSNGVKMFNQNYGIYYGDPTGSGQPYQFRYTTNGGNNWLLSPNSPLS
                     TNDYGVVNAWDWIDTAKIWLGIGNPVANATSTRIINTTSGFAGGVWNSTPITGTGENI
                     GLYYTAIAFTDANNGMAGSFEGDMRKTTDGGITWTSALNVPLTGVKRYRYMSGIKDGS
                     NVIRVSVIFGSSNYCFKTTNFGDVWTLDSLPSAGTSGGINQMEFLNGTLGYSAGNAGV
                     FMRYGNTVGINNSNSQIPSEYNLDQNYPNPFNPNTRINFRISKSNFVSIKVYNALGKE
                     MITLVNENKTAGSYSVDFDGGNF"
     gene            complement(1549..2457)
                     /locus_tag="PLX80_11650"
     CDS             complement(1549..2457)
                     /locus_tag="PLX80_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21489.1"
                     /translation="MLGKNSKEVNIDLKKFSFNDPDRISNLKEFQQNYNYSILPVDIY
                     EPVPDLRDIDITDISEDDKILDLNIKYDFSFIKENLDRILNEKIIPDWLQENPMFPVL
                     DLAAYCSIIENYKPERIIEVGAGYSTCLSTMFTDIINLKTIITAIEPYPREHLLKLEK
                     ENKITLIQNKIQDIDRSVTNQIRSLKENDILFIDTTHIVNRDSDVSYLFIKLLPMLQK
                     GVVVQIHDIFLPYDYSRSVYYGRGTFYNEQYLLASILANSNSYTSLYSSYKTFLEHFN
                     KTKIDSNSWTKPDKMRSGGSFWMQKN"
     gene            2813..3892
                     /locus_tag="PLX80_11655"
     CDS             2813..3892
                     /locus_tag="PLX80_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY21490.1"
                     /translation="MKKYKLYIVFLISCISLITFLQYIPFNKTSSFSDLSAALINSEV
                     NSVNGNYDSIKTNISSSCFDQADHKVQFIEVEKDVKLEVLDWGGKGEYLVLLTGLGDN
                     AHVFDNFAYQFTDLYHVIGITRRGFGNSDKPATGYSEERRVQDYLNVLDSLGIQSALF
                     AGHSIAGGELSQLGIYHKDRVKKLVYIDASPDFGQHKYLDQPPSVDYSEEDLMSIERF
                     IAATTRKFGYREPNSAVCNGYIIGNTGEIVDFRSPPEVSSQIIQSAEDKDFTKIDIPV
                     LALFDEFTLDYRFPFYWYLDDKKQEEYLQAWKPLVKWQQDLINRFRTDVKNINIIMLK
                     NAYHYIFINKESDVAREMRRFLMQQ"
     gene            complement(4276..5544)
                     /locus_tag="PLX80_11660"
     CDS             complement(4276..5544)
                     /locus_tag="PLX80_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HQY21491.1"
                     /translation="MKPQTKPQYIQIKDFTYDLPDDRIARYPLKERGDSKLLIYRDGM
                     ISQDVYKNIDKYLPADSLLIFNDTKVIHARLIFRNSKDEKIEIFCLEPAEDNSETVTS
                     MSNKKSVEWKCMIGRMNKWKEKVLKLRSDVLTLSAEIIEKKIDSYVIEFKWHPEELTF
                     AEVIESLGEMPIPPYLKRDSEEIDDKRYQTVYARNDGSVAAPTAGLHFSQNIINNLKF
                     KKIETDYVTLHVGAGTFKPVKSEILDDHEMHSEWIDIKKETIQKILNKLLRSDSDIKH
                     KGIITTVGTTSLRSVESLYWMGVKANLNPDSKLHEIEIKQWDPYEIDTELSAVESLNS
                     ILVWMEKNNLYRLICKTQIIIVPSYKLKIADALVTNFHQPDSTLLLLVAAVAGNDWKE
                     IYKYALENGFRFLSYGDGSLIFKNENSGIT"
     gene            complement(5636..6328)
                     /locus_tag="PLX80_11665"
     CDS             complement(5636..6328)
                     /locus_tag="PLX80_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HQY21492.1"
                     /translation="MKWTYLIINIAAIFVPFIYSFHPKILFYKQWKFFLPTLLIVSAI
                     FIIWDVIFTANGIWWFNENYISGFYIFNLPIEEILFFICIPYACAFTYYCFKKFLTLK
                     FFNNKSRYISIIVITGLLISGIIHFDKAYTVTTFLSLAIVLTIVTFILKQNWLGNFYF
                     TFMILLIPFFIINGILTGTGPDAPVVFYNENEIIGIRIMTIPVEDIFYGMELILLNIL
                     GMEILNKLSLKK"
     gene            complement(6329..6787)
                     /locus_tag="PLX80_11670"
     CDS             complement(6329..6787)
                     /locus_tag="PLX80_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY21493.1"
                     /translation="MIIINIFTVIVSFAFMEFIAWATHKFVMHGFLWTLHKDHHTKES
                     SGFFEKNDFFFLIFAIPGMLLIYFGLNNGFDIMFFSGIGITLYGLAYFLVHEIFIHQR
                     FKFLKKSESGYLNAVRRAHKIHHKHLQKENGECFGMLWVTSKFYKNTSQS"
     gene            complement(6784..7269)
                     /locus_tag="PLX80_11675"
     CDS             complement(6784..7269)
                     /locus_tag="PLX80_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY21494.1"
                     /translation="MFELKREQLIHSDLKTVWTFFSSPINLSEITPPELDFRIITETE
                     DTVYEGMEIEYRVKPVLGIPVKWISLIKDVDVPFAFTDEQLTGPYKVWIHKHEFSETN
                     NGVLVKDHIQYQLPFGSLGTYLEKIIVRKKLTEIFNFRSKIIKNIFPESKIPEFELKN
                     I"
BASE COUNT         2434 a         1195 c         1094 g         2547 t
ORIGIN      
        1 aaaattacct ccgtcaaaat ccacggaata actcccggct gttttatttt catttactaa
       61 agttatcatt tcttttccaa gagcattata aacttttatt gatacaaagt tggattttga
      121 aatccggaaa tttattcttg tatttggatt aaacggattt ggataattct gatcaaggtt
      181 atattcagat ggaatttgac tgttactatt attgattcca acagtatttc cgtatctcat
      241 aaacactcct gcatttccgg ctgaatatcc aagtgttccg tttaaaaatt ccatttgatt
      301 gattccacct gaagttccgg cagatggtaa agaatcaaga gtccagacat ctccaaaatt
      361 agtagtcttg aagcaataat tactggatcc gaaaattaca ctgacccgga tcacatttga
      421 accgtccttg atcccggaca tatacctgta tctttttact cctgttaaag gcacattcag
      481 agctgaagtc catgtaattc ctccgtcagt tgtttttctc atatcacctt cgaatgaacc
      541 tgccattccg ttattggcat ctgtaaatgc gatagcagta taatataatc caatgttttc
      601 accggttccg gtaattggtg tagaattcca gactccaccg gcaaatcctg aagttgtatt
      661 tataattctt gttgaggttg catttgcaac aggatttccg ataccgagcc atatctttgc
      721 agtatcaatc cagtcccatg catttactac tccgtaatca tttgttgata acggtgagtt
      781 tggtgaaagt aaccagttgt ttcccccgtt agttgtatat ctgaactgat aaggctgccc
      841 tgatcctgtc ggatccccgt agtaaattcc ataattttga ttaaacattt ttacaccgtt
      901 actaaaactt ccggctaaag aaaactgaac agtccagttc ataccgccgt ttgaagtatg
      961 ataaatacta cctgtttcag ttccaaccca gcaactggaa gtatccaaag cagagatccc
     1021 gattagacct gtaaccggta atcctgaatt tctcagcatc cagtttacac caccatttac
     1081 tgtcttgtaa agaactcctc cgttacagga aacccagatg acattctgat cggctgcaga
     1141 aattgagttt acacttggat tacccggaat tctgcttata aaagtccatt gactgaaagc
     1201 actggtagat gctaacataa agataaacag gaaaaatttt ttcatttaga ttattattat
     1261 aggagtaata aatattttcg gataatatat aatataattt tgtcaagtat aatgtaaatt
     1321 tatgaatgtg aaaattgttt aaaagaaaat tataaatgaa taagtttttt tactcactat
     1381 cacaggcatt gtgcgatctg cataataaag aagtgctgta ttatatgaac ttggcaaaaa
     1441 tcgaatcaat gattgtgttt atatttctca agtaataaat aaaaattgtt tattactccg
     1501 cagagcacgt gactttgagt aaaaaattat tgacgtgtta tacaaacatt aatttttctg
     1561 catccagaaa ctcccaccgc ttctcatttt gtcaggtttg gtccaactat ttgaatctat
     1621 tttagtttta ttaaaatgtt ccagaaaggt cttataagat gaatatagtg aagtgtaact
     1681 gttgctgtta gcaaggatag aggcaagtaa atattgttca ttataaaagg tacctcttcc
     1741 ataataaacc gatctgctgt aatcataagg aagaaaaata tcatgtatct gcaccaccac
     1801 acctttttgc agcataggca gaagttttat aaaaagataa ctgacatctg aatccctgtt
     1861 cacaatatgg gtcgtgtcaa taaataaaat atcattttct ttcagtgatc ttatctgatt
     1921 tgttacactc cggtcaatat cctgaatttt attttgtatc aatgtaattt tattttcttt
     1981 ctcaagttta agcaaatgct ctctcggata tggctcaatt gcagtaataa ttgttttcaa
     2041 atttattatg tctgtaaaca tagtagaaag acatgtggaa tatcctgcac cgacctcaat
     2101 gattctttcg ggtttgtaat tttctattat cgaacaatag gcagccagat caagtacagg
     2161 aaacattggg ttttcctgca gccagtcagg aatgatcttc tcattaagta ttctgtctaa
     2221 attttctttt ataaaagaaa aatcatactt tatatttaaa tccagaattt tatcatcttc
     2281 tgagatatca gttatatcaa tatctctgag atctggaaca ggttcataaa tatcaaccgg
     2341 gagtatgctg taattataat tttgctgaaa ttcttttaga tttgatattc tgtccggatc
     2401 attgaaagaa aattttttca gatcaatatt aacttctttt gaatttttgc cgagcaattt
     2461 attttttatt ttttttaaca attgtaacag taaattgatg aatcaatatg aaaaactttt
     2521 gaaagaaatc tttaattttc agttgcttat aaaatatgaa ttttttctat aaaaaaatat
     2581 agcgatatta tcttaataaa atgttgtcgg aagaatttac attataatca ttaagaataa
     2641 tttttataga aaattttcag attacagttt tagtttttga taccaaatat tatacaatga
     2701 caattattta aacattttct cttcatgcag attcaaattt taaactatgg atctgtcaat
     2761 accggatatt aaaatcttct gaagaaatta aaaaaaatta aaatatatct aaatgaaaaa
     2821 atacaaactc tatattgtat ttcttataag ttgtatttcc ttaattactt ttttacaata
     2881 tatccctttc aataaaacct catctttttc tgatttatca gcagcactta taaattctga
     2941 agtaaattct gttaatggaa actatgacag cattaaaact aatattagta gtagctgctt
     3001 tgatcaggca gaccataaag ttcaatttat tgaagttgaa aaagatgtaa agcttgaagt
     3061 attagactgg ggcggtaaag gtgaatatct tgtattgcta acaggtctag gagataatgc
     3121 tcatgtgttt gataattttg cttatcagtt cacagacctg tatcatgtta tcggaattac
     3181 gcgcagaggt tttggaaatt ctgataaacc tgcaacaggt tatagtgagg aaagaagagt
     3241 ccaggattat cttaatgtac tcgacagtct gggaatacag tcagcattgt ttgccggtca
     3301 ttcgattgca ggcggtgaat tgtcacagct tgggatctat cataaagacc gtgtaaaaaa
     3361 actagtttat attgacgctt ctcctgattt cggacaacat aaatatcttg atcagccacc
     3421 atcagtggat tacagcgagg aagatcttat gtcaatagaa agatttattg ctgcaactac
     3481 gagaaagttt ggataccgtg aaccaaactc tgctgtctgc aatggttata ttattggaaa
     3541 tactggagaa atagttgatt tcaggtcacc gcctgaagtc tcctctcaga ttattcaaag
     3601 tgctgaagat aaagacttta caaaaattga tattcctgtg cttgcacttt ttgatgaatt
     3661 cactcttgat taccgatttc ccttttactg gtatctcgat gacaaaaaac aggaagaata
     3721 tttacaggct tggaaaccac ttgtgaaatg gcagcaggat ctgataaata gattccggac
     3781 agatgtaaaa aatattaata taataatgct gaagaatgca tatcattata tatttattaa
     3841 taaggagtca gatgtagcaa gagagatgag aaggtttttg atgcaacaat aatccctgtc
     3901 caatacaaaa attgaaagaa atcgatagaa tattttaaat tatatataaa cctgattctt
     3961 taccaaagga ataatttaaa cctgaatttt ttattcagca catattgaaa cagtattttc
     4021 tcaactgcac aaatttccca tgaccatata gatgaagaaa atttgatcca gatcaattct
     4081 tctgataact tacagcattt tggaaattta ttgtggtgtt caattttatt acactttaat
     4141 ctttaatctt ctatttctgc aagctaaaat atctacctgt ggaaattctg attcaaactt
     4201 ataataaggt tcgaaattaa taaaaataga tttaatcaaa tatgttttat ataaattttt
     4261 atgtacagaa aatatttaag ttatacccga attttcattt ttaaaaatta aacttccatc
     4321 accataactc aaaaacctga acccattttc caaagcatat ttataaattt ccttccagtc
     4381 attacctgca actgctgcaa ccaatagaag caatgtcgaa tcgggctgat gaaaatttgt
     4441 aactaatgca tcagctattt ttaatttgta agaggggact ataatgatct gagtcttaca
     4501 aattaatctg tacagattat tcttttccat ccatacaaga attgaattta aggattctac
     4561 agcagaaagt tctgtatcaa tctcataggg atcccattgt ttaatttcaa tttcatgaag
     4621 ttttgaatca ggattcagat tcgctttcac ccccatccag taaagtgatt ctacagatct
     4681 cagtgaagta gttccaactg ttgtgataat tcctttatgt ttaatatctg aatctgatct
     4741 taaaagctta tttaaaattt tttgaatggt ttcttttttt atatcgatcc attccgaatg
     4801 catttcatga tcatcaagaa tttcactttt cacaggtttg aatgttcctg ctccgacatg
     4861 aagtgtaacg tagtcggttt ctattttttt aaattttaaa ttgttaatta tattttgaga
     4921 gaaatgtaaa cctgccgttg gtgcagcaac agagccgtca tttcgggcat agacagtctg
     4981 atatctttta tcgtcaattt cctcactgtc tcttttgaga taaggtggta ttggcatttc
     5041 acccaagctt tctattactt cagcaaatgt aagctcttcc ggatgccatt taaattctat
     5101 tacataagaa tcaattttct tttcaataat ttcagcactc agagtcagta catctgatct
     5161 cagtttaagt actttttctt tccacttatt cattcttcca atcatacatt tccattcaac
     5221 acttttttta tttgacattg aagttactgt ttcagaatta tcctcagcag gctcaagaca
     5281 aaaaatttca atcttttcat ctttggaatt tctgaaaata agtctggcat gaattacttt
     5341 tgtgtcatta aatatcaaaa gtgaatctgc gggtaaatac ttatcaatgt ttttataaac
     5401 atcttgagaa atcattccgt ctctgtagat cagtaattta ctatcacctc tttcttttaa
     5461 tggatatcgg gcaatccgat catcaggaag atcgtaagta aagtctttta tctgaatata
     5521 ctgaggtttt gtttgtggtt tcatattgat actgcagaaa tgatgatttg aatttataaa
     5581 ttacttttaa aatggatcta aatttgttgt ttaaattata actcaattca agtatttatt
     5641 tctttaatga caatttattt aaaatttcca ttcccaaaat atttaatagt atcagctcca
     5701 ttccataaaa aatatcttca acaggtattg tcattattct tattcctatt atttcatttt
     5761 cattataaaa aaccacaggt gcatcaggtc cggtaccggt taggattccg ttaataataa
     5821 aaaagggaat cagcaaaatc ataaaagtaa aatagaaatt cccgagccaa ttctgtttta
     5881 aaatgaaggt aacaatggtc aatacaattg ccagacttaa gaatgttgtc acagtatatg
     5941 ccttatcaaa atgaataatt cctgagataa ggagacctgt aattacaata attgagatat
     6001 atctgctttt attattaaaa aattttaatg ttaaaaattt tttgaagcag taataggtaa
     6061 acgcacatgc ataaggaatg cagatgaaga acagtatttc ctctatgggt aaattgaaaa
     6121 tataaaatcc ggaaatataa ttttcattga accaccaaat cccattagct gtaaagatta
     6181 catcccatat tatgaatatt gccgaaacaa tcagtagtgt cggtaagaaa aatttccatt
     6241 gtttgtaaaa caggatcttt ggatgaaatg aatagataaa aggaacaaaa atggcagcaa
     6301 tattaattat cagatacgtc catttcattt atgattgtga agtattttta taaaattttg
     6361 atgttaccca tagcattcca aagcattcac cattttcctt ttgaagatgt ttatgatgaa
     6421 ttttatgtgc tcttcttact gcatttaaat atcctgattc agatttcttt aaaaatttaa
     6481 atctctgatg aatgaaaatt tcatggacaa gaaaatatgc caatccgtaa agtgttatac
     6541 caatccctga aaaaaacata atatcaaatc cattattcaa accaaaatag ataagcagca
     6601 tacccggaat agcaaatatc agaaagaaaa aatcattttt ttcaaaaaaa ccagagcttt
     6661 cttttgtgtg atgatcttta tgcaaagtcc ataaaaaccc atgcattaca aatttatgag
     6721 tagcccatgc aataaactcc ataaatgcaa aagatacaat aacagtaaat atgtttataa
     6781 taatcatata ttctttaatt cgaattccgg gattttactc tccggaaaaa tatttttaat
     6841 aatcttactt ctgaagttaa aaatttcagt gagttttttc cgtacaataa ttttctccaa
     6901 ataagtacct aagctgccaa agggaagctg atattgaata tgatctttta ctaatacgcc
     6961 attgttagtt tcagaaaatt catgtttatg tatccagact ttatatggac ctgttaactg
     7021 ctcatctgta aatgcgaacg gaacatcaac atcttttatc aatgatatcc attttacggg
     7081 aattcccaaa accggtttga cacggtattc tatttccatc ccttcataaa ctgtatcttc
     7141 agtttctgtg attatcctga agtcaagttc aggtggagtt atctctgata aatttattgg
     7201 tgaagagaaa aatgtccaga ctgtttttaa atcagaatgt ataagctgtt ctctttttaa
     7261 ctcaaacatt
//
LOCUS       DAORVQ010000169         7235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6234_length_7235_cov_5.083983, whole genome shotgun sequence.
ACCESSION   DAORVQ010000169 DAORVQ010000000
VERSION     DAORVQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_7235_cov_5.083983"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            643..1410
                     /locus_tag="PLX80_11680"
     CDS             643..1410
                     /locus_tag="PLX80_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQY21495.1"
                     /translation="MIFSTASAQKKATEDQQANELAKKLANPVSNLISVPFQNNFDYN
                     FGTLGGYRYTLNFQPVIPMKLGKSLSLINRFIVPFINQNNVTGPEMQNGLGNTLYSAF
                     FSPKTGKLIFGVGPEIGFPSTNKYLGAENWLIGPTLVVLAQPGNWSIGVLSNNLWAVN
                     VDSSKSNVNNLYFQPFISYRFDGAFTVGISSEDTYDWLHKRLNSGMIALNMSQILKIG
                     GKLPASIEVTPKYFFASNQVVSPDWGIRAALTLLFPK"
     gene            complement(1656..3674)
                     /locus_tag="PLX80_11685"
     CDS             complement(1656..3674)
                     /locus_tag="PLX80_11685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21496.1"
                     /translation="MNKKTYKGFSYFRSLIFLALIIFVIVFQGQQSGSVFDDDNPYIE
                     QPTMESYMNYRNNNQTHTPSIITVDGYDNFDIGVDNWEQNMTINPLNPLEIFFGANAN
                     PQNARYSTNGGLNWTVSNPSYHSSTCCDPWSSYTGNGVLIYASGVNGQYVYRSTNNGM
                     NWSTPVLSVAGNDRNHVSAEYTGNGPYANYVYAGITPGNFGRSTDAGATWTTTFTPSN
                     SLPGCYIAVGPNGAVDGGCVIYVTNTGSVNARTYNFYRSTDGGATFTLRSSQNYAAVV
                     GTANTAGRHVINNARTNPHPKIAMDNSNGPYRGRLYLVYANNNPPASGNKPDIFLRYS
                     TDQGATWSDTNRVNDNINPMLSDQWFPEIYCEKDNGRLYIHWYDDRNNPVNYGTDIYA
                     TYTEDGGQTFEENQRLTNTTFLYPRPVCAVNSNCYRGDYTSIAGNKDAAYSVWGDHRN
                     GNAQNMGGYFPDFALTVSPESDTLRGIADSSFHNVKIPGVKLWDKSTVFSAIVNPVPA
                     SGSISMTFLNNSTNIPNDSLTSYPDSLRLRVVTSGAVPTGVYTVMVKGNGRNATPVHV
                     RNISITVDNPVGIISNQTAVSFDLYQNFPNPFNPSTSIIYELPQSELVTLKIFDALGK
                     EVTNLVNSRQSAGVYSVDWNAGNHPSGIYFYTIQAGNFKETKRMMLLK"
     assembly_gap    3773..3872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3912..5201)
                     /locus_tag="PLX80_11690"
     CDS             complement(3912..5201)
                     /locus_tag="PLX80_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21497.1"
                     /translation="MKKFYFALLVFFTTVSISSSQILFSENFNYNTRDTLNNVGGWQS
                     SSNSNRYVKVISSGLTYPGYLSSGIGNCASFANIPVSSLSINYFGNENSGTVYLSYMI
                     RVDSLATGVTEGSVISLDEAGGSTNNNTLLYVKKVTSSTFNFGIRKYSGATIYSPTIY
                     NKNTTYLIVVSYNFIAGASNDVSKLFVFTSGVPATEPAVPSAVDTVGNDVNSVGDVTI
                     QNSYIESGLNGSSMKLDGIRIGKSWNSILFQNYVCKLSMKALIQGFYNDVTNKMVKDT
                     VTVSLRYNRPPYTIADTKKSVLDSNGNGTFNFTKIGNLTPYYIQFDHRNTLETWSKPA
                     KEFGNNTLSHDFTIGVNQSYGNNAIFKGSKACFYNGEILHDGIIDVTDYQFVDNNSFN
                     FAAGYKTTDLNGDNITDIDDLAIVELNAFNFVQVIRP"
     gene            5583..7040
                     /locus_tag="PLX80_11695"
     CDS             5583..7040
                     /locus_tag="PLX80_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21498.1"
                     /translation="MISTNITDILKTFSEDEIKKFKSFIASPYYNKNKNVNAFFDQII
                     KFHPEYNSVKLSKLSVFEKIFGKSKYNDGLIRSLSFNLNNLLKNFLIDQKNANEDLKN
                     ELLLLEEYNRRKLNKHFLKKFEELNDTLNNRKNKDEIYYECKVKMQRQMDYFVEINRF
                     NQKDYNVFKNYSISSMSENLSFYICHLVISNNSLIFEETDESSNSLDSDLIKYIFKEI
                     LSEKSKYKENTLINHNRLQILINRERKEEHYQELKKIFLTGFDLLDHFERYNIHNLLQ
                     HFCIDKIFEGKAEYRKERFELYKASLDQKLFSLYEDEYFNDMLFGNIVIVAIAVSEID
                     WLEDFIKQYIPLLSPDVKDTIEKYSTAKVSFAKKNYQETLIHLNTIENSNYVRNETSL
                     RDLKMMSLYELGLFNELHYQFDAYNHFLKKNKKLVTGLRFTRIKNFLKYFQMLLKANE
                     RSDKKIFTELQYELNENPNVMERRWLLSKLSEIKI"
BASE COUNT         2409 a         1158 c         1136 g         2432 t
ORIGIN      
        1 gttagtaatc aatatatacg tttttcaaat cttaaaacat acactgatat tgcctacatt
       61 aaaaatcata atataaaata aaagctccat cctttgtcca aatggcgaat gggagctatt
      121 ccattaagtt aagtgattta tattttataa ttttattaat gtaaatttat aattaaaaaa
      181 ttcaggttct tccccctttt tcaaagggaa gatgggagag gtttgctgat agcgacgata
      241 ctttcattaa cttaatggca ttgagaccgg gagagtttgt aaagctgagg ttcaattaat
      301 cctgctgcat taggggaaat atttgtaaat tattttaaat cgtgctgtaa aaattccggc
      361 ttataactta aaataatcga tgtcttaaat agtccgggat attaaaagaa tttatttgga
      421 atttatttgg aatttatttg gaatttattt taatcgtatc ttttctaaat ttccaaaaat
      481 tattttcaat tgaatacatc tcccccgaaa tgtatttgat tttctaaatt aaaagtactt
      541 attccacaat gaatttttaa tttaaactaa cttaatataa acctacttat gcaaattatt
      601 acttcctcca aaacaatttt ttatctgtta atttttttga taatgatctt ttctacagcc
      661 agtgcccaaa aaaaagcaac tgaagatcag caggcaaatg aactggctaa gaagcttgct
      721 aatccggttt caaatcttat cagcgtcccg tttcagaata actttgatta taatttcggt
      781 acgcttggcg gttacagata tacattaaat ttccagccgg tgattccaat gaaactggga
      841 aagagtctta gtttgataaa cagattcatc gttccgttta taaatcaaaa taatgttacc
      901 ggacctgaaa tgcaaaacgg tttaggaaat actttgtact ctgcattctt ttcaccgaaa
      961 acaggcaaac ttatttttgg agttggtccc gaaattggat ttccttcaac caataaatat
     1021 ttaggtgcgg agaactggtt aatcggtcct acacttgttg tacttgcaca gcccggaaac
     1081 tggtcaattg gtgttttaag taataatctc tgggctgtca atgtggattc aagtaaatca
     1141 aatgtaaata atttatattt tcagccattt atcagttaca gatttgacgg cgcatttact
     1201 gtcggaattt cttctgaaga tacttatgac tggctgcata aaagacttaa ttcaggaatg
     1261 atagcactca atatgtcgca gatcttaaag atcggaggca aacttcctgc cagcatagag
     1321 gtcactccaa aatatttctt tgcaagcaat caggtggtaa gtccggactg gggaatcagg
     1381 gcagcattga ctcttttgtt tccgaaatag aattttacag aacaccggaa actgattttg
     1441 aatagtcttt atagatgaag aaattgattt gaatgatttc gcattgcatg ctttttaatt
     1501 catttaatat taaagataat tattctttgc tacggtatat cattccttta atataaaaaa
     1561 cttccggaat ctattttcaa agaccccgga agtttaattt tttaaattta atatttatta
     1621 tttaaaattt attatttaaa atttattatt taaaattact tgagcagcat catacgttta
     1681 gtttctttaa agttacctgc ctgtattgta taaaaataaa ttccgctcgg atgattgcct
     1741 gcattccagt caacggaata cacacctgct gattgcctgc tattaacaag attagtcact
     1801 tcttttccaa gtgcatcaaa tattttcaga gttaccagtt cagattgcgg aagctcataa
     1861 attatcgatg tcgaaggatt gaacggattc ggaaaattct gataaagatc aaaacttact
     1921 gctgtctgat ttgaaattat tcctaccgga ttatcaactg taatactgat atttctaaca
     1981 tgaacaggcg ttgcgtttcg accgttacct tttaccatga ctgtataaac accggttgga
     2041 actgcgccgg atgttactac tcttaatcga agtgaatcgg gataagaagt caatgaatca
     2101 tttggaatgt ttgttgaatt atttaaaaaa gtcattgaga tactgcctga agccggaacc
     2161 ggatttacta tcgctgagaa aactgttgac ttatcccaga gttttacacc ggggattttt
     2221 acattatgaa atgaactgtc agctattcct ctaagtgtat ctgattccgg cgaaactgtc
     2281 agagcaaaat caggaaaata accgcccatg ttctgcgcat ttccgtttct gtgatcaccc
     2341 cagacgctgt aagctgcatc tttatttccg gctatgctgg tgtaatctcc tctgtagcag
     2401 tttgaattta ctgcacagac cggacggggg tataaaaatg ttgtattcgt aagtctttga
     2461 ttttcttcaa atgtttgtcc gccatcctca gtataagttg cataaatatc tgttccgtaa
     2521 tttacaggat tatttctgtc gtcataccag tgaatatata atctgccatt atctttctca
     2581 caataaattt ccgggaacca ctgatcacta agcatcggat taatattatc gtttacccta
     2641 tttgtgtccg accatgtagc tccttgatca gttgaatatc ttaaaaaaat gtccggttta
     2701 tttccgcttg ccggaggatt gttatttgca taaaccagat acaatctgcc tctgtaaggt
     2761 ccgttactat tatccatagc gatctttgga tgaggatttg ttcttgcatt attaataaca
     2821 tgtcttcctg cagtatttgc agtaccgact actgcagcat aattctgaga tgaccttaaa
     2881 gtaaatgttg ccccgccgtc tgtcgatctg taaaaattat aagtccttgc attaacagat
     2941 ccggtatttg ttacataaat tacgcatcct ccatcgactg ctccatttgg acctacagca
     3001 atgtaacatc ctggcagaga attacttgga gtaaatgtag tagtccatgt tgcaccggcg
     3061 tcagtacttc tgccgaaatt tccgggagtg attcctgcgt acacgtaatt tgcataagga
     3121 ccatttcctg tatattcagc cgagacatga tttctgtcat tacctgcaac agaaagaaca
     3181 ggagtactcc agttcattcc gttatttgta gatctgtaaa catactgacc attaactcct
     3241 gatgcataga tcagaactcc gttaccggta tatgaactcc agggatcaca gcatgtagag
     3301 gaatgatagg acggattact aacagtccag ttcaatccac catttgtaga atatcttgca
     3361 ttctgcggat ttgcattcgc tccaaaaaaa atctcaagag gatttaaagg attaatggtc
     3421 atattctgtt cccagttatc gactccaata tcaaaattat catagccatc aactgtaatt
     3481 atactcggtg tatgagtctg gttattattt ctgtagttca tataagattc catagtaggc
     3541 tgctcaatgt aaggattatc atcatcgaag acagatccgg attgctgccc ttgaaatact
     3601 attacgaaaa ttattaaggc aaggaaaatt aaagatctaa aataactaaa acctttgtaa
     3661 gtttttttat tcataacttt tgtaaattaa aaaaattgtt ttgagaattt agttatttca
     3721 tttgattaaa tcttgtcaat tttgtttttt ttgtcgtagt tcagattatt aannnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcttaag gcagattatt aatctgcctc
     3901 tacaattgca cctatggacg tatcacctgc acaaaattaa atgcattcaa ttcgacaatt
     3961 gccagatcat caatatctgt aatgttatca ccattgagat cagtagtttt atatcctgct
     4021 gcaaaattga atgaattatt atctacaaac tgataatcag ttacatctat tatcccgtca
     4081 tgaagaattt caccattgta aaaacaagcc ttactacctt taaaaattgc attatttcca
     4141 tatgattgat tgacacctat agtaaaatca tgtgataaag tattattacc aaattcttta
     4201 gctggcttgc tccatgtctc aagtgtattt ctgtgatcaa actgaatata gtatggagta
     4261 agatttccga tctttgtaaa gttaaaggta ccgttgccat tagaatccag cacagatttt
     4321 tttgtatccg caattgtgta cggaggtctg ttatatctca atgatactgt gactgtatct
     4381 ttcaccatct tatttgtgac atcattataa aatccctgga tcagtgcttt cattgaaagc
     4441 ttacaaacat aattctggaa taatatactg ttccatgatt tgcctattct gattccgtcc
     4501 agtttcattg agctgccgtt cagtcctgat tcgatataag aattctgaat agtaacatct
     4561 ccgacactat ttacatcatt ccctactgta tctactgcac ttggtactgc gggttctgtt
     4621 gccggtacac cggaagtgaa aacaaataat ttagaaacat cattagaagc tcctgctata
     4681 aaattataac tgactacaat taaataagta gtatttttat tatatatcgt cggggaatat
     4741 atagtcgcac cactatattt cctgattcca aaattaaatg tagaagaagt aaccttcttt
     4801 acatataaaa gtgtattatt attagttgat ccgccggctt catccagact tattacagat
     4861 ccttctgtca cgcctgttgc aagactgtcc acacgtatca tatatgacaa ataaactgtc
     4921 cctgaatttt cattaccaaa ataatttata gataaactgc tgactggaat attagcaaac
     4981 gaagcgcagt tacctattcc cgaacttaaa tatccgggat atgtcaaacc ggaagatatt
     5041 acttttacgt acctgttgga attactagaa ctttgccagc caccgacatt attcagagta
     5101 tctcttgtat tataattaaa attttctgaa aataaaatct gagatgatga aattgacact
     5161 gtagtaaaaa aaacaagtaa agcaaaataa aattttttca tttttaaaat tttttttagt
     5221 taattaatat tgcctttcgg ctgtccctct tattttataa aagggacagt ttcagggctg
     5281 cttccagggt gactgttata aggacaaatt aggtttgccc ttaagatgga agtaatattt
     5341 aaaattattt ttcggagtca ccctgaaaaa tctttacaaa aatactgaat ggtctagatg
     5401 caaatcaaag aattatttct tcaaatgttt ttgataatac caggcttaat tttgattttt
     5461 ttgataaatt attcaaatac ggtctgtttt caatggtttt tcaataatca gatgtgtaaa
     5521 tttgttttta ttttttagga aaaattttta ataaaaatga taataataaa caaaacaaag
     5581 cattgattag cacaaatatc acagatattc ttaaaacttt ttctgaagat gagatcaaga
     5641 aattcaaatc atttatcgct tctccttatt acaataagaa taaaaatgta aatgcttttt
     5701 ttgatcagat tattaaattt catccggagt acaattccgt taaactttct aagttatctg
     5761 tctttgaaaa aattttcggt aaatcaaagt ataacgacgg tcttataagg tctttaagct
     5821 ttaatcttaa taatctgctg aagaattttt taattgatca gaaaaatgcg aatgaagatc
     5881 tcaaaaatga actcttatta ttagaagaat acaaccgtcg aaaactcaac aaacattttc
     5941 taaagaaatt tgaagagctt aatgacactc ttaacaacag aaaaaataaa gatgaaattt
     6001 attatgaatg taaagtaaag atgcagagac aaatggatta ttttgttgaa ataaacagat
     6061 ttaatcagaa agactacaat gtgtttaaaa attattctat ttcatcaatg agtgaaaatc
     6121 tttcatttta tatctgccat cttgtaattt caaataacag tctgatattc gaggagacag
     6181 atgaaagcag caatagcctg gattcagatt taatcaaata tatttttaaa gaaattctat
     6241 ctgaaaaaag taaatacaaa gaaaacactc tgattaacca taaccggctt cagatcctta
     6301 ttaacaggga aagaaaagaa gaacattatc aggaattaaa aaaaatattc ttaactggct
     6361 ttgatttgtt ggatcacttt gaaagatata atattcataa tttgcttcag catttttgca
     6421 tagataaaat atttgaaggt aaagctgaat acagaaaaga aaggtttgaa ttatataaag
     6481 catctcttga tcaaaaatta ttttcacttt acgaagatga gtattttaat gatatgcttt
     6541 ttgggaatat agtaatcgtt gcaatagctg tatcagagat cgactggctg gaagatttca
     6601 ttaaacagta tataccttta ttatctcctg atgtaaaaga tacaatagaa aagtactcaa
     6661 ccgctaaagt ttcttttgca aaaaaaaatt atcaggaaac tttgattcat ttgaacacaa
     6721 tagaaaattc aaattatgta agaaacgaaa catctttgag agatcttaaa atgatgtctc
     6781 tctatgaatt aggtttattc aatgaattac attatcagtt cgatgcttat aatcattttc
     6841 tcaaaaagaa taaaaagtta gttacaggat tgagatttac gcgaataaaa aatttcttaa
     6901 aatattttca aatgctttta aaagcaaatg aaagatcaga taaaaaaata tttacagaac
     6961 ttcaatatga gcttaatgag aacccaaatg taatggaaag aagatggctt ttgagtaaat
     7021 tatctgaaat taaaatttaa cttaatgaat attataagtg ctctaagttt aataagttta
     7081 tggaaatttt ataaagtcaa gattcatttt taaagttctg caataatttt ttatataatc
     7141 tcataagttg cttaaagtta agatagataa gatagataag ataaataaaa aagataagaa
     7201 ggaaataaga aaaaaggtga tcattacagg aagtg
//
LOCUS       DAORVQ010000170         7082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6451_length_7082_cov_4.258859, whole genome shotgun sequence.
ACCESSION   DAORVQ010000170 DAORVQ010000000
VERSION     DAORVQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6451_length_7082_cov_4.258859"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLX80_11700"
     CDS             <1..431
                     /locus_tag="PLX80_11700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21499.1"
                     /translation="FHIYGDGEKRIEFEKLIIDYGLEKSVFLKGYTKDVLSVLRTFDL
                     FVLTSRYEGIPYVILEAMSMNIPIVTTDVGGISEVIKNKINGLLAENGNVSDISEKII
                     YIMENDELRIQFIDKARKDFEEKFLIENTVSQIEKIYEMK"
     gene            447..1604
                     /locus_tag="PLX80_11705"
     CDS             447..1604
                     /locus_tag="PLX80_11705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY21500.1"
                     /translation="MKDKIKLVHIISNLSLGGAQVLLFDILHYLSKKNDLEIYVITID
                     SGEYIEKFKKAGIKVFDLMEKGLVNPKIYFKMKKLLKYLNPDIVHTHLNKADFYGRIA
                     AKKTGVKLIYSTCHNYSTHHKGADVNKISIFDYIDNFVVKYSGSYLIAISEIVKKYLI
                     NRDKRFDSITDVIYNGVNISKKNYQLEENEIDLLRIKYNVSKDDFLICLSGRFDKQKG
                     HLFLLDALSEKIKERKNIKIIMPGDGILKKEVENYISKNDLNEFVILPGFHKDIEKII
                     EISDLTIVPSLWEGFGLVVIESMVKKKVVLASDTGGIPEIIEHGENGYLFKTLNKSDF
                     LEKFNFIYSVNSNLPEIQTKALNTVEMRFDINKNSEFYYLSYLSKFQSISK"
     gene            1621..2394
                     /locus_tag="PLX80_11710"
     CDS             1621..2394
                     /locus_tag="PLX80_11710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21501.1"
                     /translation="MTENLCPACGSSDVKNCGQPIYSDKGSKYSVIECNICQLNWCNP
                     MPTIDELDEHYLKYYEVRHNTVEKSAMKLAIRKKITFREYRLKKFFSLIKKFSPVNYI
                     LDFGCGEAEILYLAKKKQWQVLGVDYSNELSEKFNKENINFIQANDLFSINIEKSSVG
                     CISAKHTIEHLTNLEFFFDSTRDILHKDGILAIKTPSSTSLRAKRGLSGWHHVNPPEH
                     CWGFNLNNFGKLVSRNGFEVVYLKDGILVNELTCIARKI"
     gene            2464..3318
                     /locus_tag="PLX80_11715"
     CDS             2464..3318
                     /locus_tag="PLX80_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQY21502.1"
                     /translation="MNKLLSKILFKTVFVILPLILVVIVSNIILSNNSEEVKNDKFKW
                     QLTFEDNFDSFDRSKWQTMFDMNTRTIWSNKELQWYKDENVFADNGVLKIITKKESIF
                     GKDAESEKQFEFTSGMINTARTFTQSYGKWEIKVKFPYKKGYWPAFWLVAKERPGLPE
                     IDVFEYFGRKENSMSSSLHWGINYPSSEKDNSAPFYYVKSNEITGDFSDNWMIWSLEC
                     FPDKILWKLNDKIVFESTQGIPTGPMYIIANVAIKAWDENNGEVDRSDDPYVMEIDYI
                     KAYKIIPE"
     gene            3425..4345
                     /locus_tag="PLX80_11720"
     CDS             3425..4345
                     /locus_tag="PLX80_11720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21503.1"
                     /translation="MNFKFKAKIQNLFSVLPKGENINYFFQKHVTKSLPISDEDFKIK
                     LETVKLHFDNYVKYSGNIDFKRSRYYEFGSGYDLVIPIGIRSLGFDELTCIDIRELVF
                     PELINDTINRFNKTAGKISDEFCLFENVPTVTGSNYKNVLSEYFNINYIAPLDARNTG
                     IESDSIDFILSNATLEHIPEIHIEDILKECNRILKPGGIMSNAIDYRDHWSFFDNNIS
                     IYNYLLYSENEWKKLNPSIMYQNRMRHKDHIQKIKNAGFEIISEKLDLADDSEIEKLK
                     TINLNDHFTNNYTFEEIAIKSSMLILRKNN"
     gene            4409..5245
                     /locus_tag="PLX80_11725"
     CDS             4409..5245
                     /locus_tag="PLX80_11725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21504.1"
                     /translation="MSSNSVPRVSIIIPTYNRVDMLEKSIESVFSQSFTDWELIIIDD
                     ASTDGTEERMTKLSEREPAINYMRIPRITGKGISEYLNIGLRNSRGEYIARIDDDDFW
                     CHKDKLKMQVEFLDKNPEYVVVGGGVILVDKDGKELFKYLKKETDEEIRSFALFSNPF
                     THATVMFRKETAMKLGGYKNLEHVEDMELWLRMGMEGKLYNFKEYFITYMTAGQNKSF
                     TQQRENSRTVVNVVKMHGKNYPNYYKAYLLNYTQYMYSFLPVFFKKRLQSFMYYLKRK
                     NF"
     gene            5268..6251
                     /locus_tag="PLX80_11730"
     CDS             5268..6251
                     /locus_tag="PLX80_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY21505.1"
                     /translation="MGKKYFIAGGAGFVGSHLCRDILKNEPHAEITIYDNFSSGQMWH
                     IDDFKVKIKIIKADIKEKDTLNDAMKDQEVVYHFASNPDIAKAMTQPDIDFWEGTYLT
                     NNIIEAMRINSVKNLIYASGSGVYGDVGFTETAEDFSPQLPISTYGSSKLACEAMIGS
                     YCYMFDMNAAAFRFANVVGPNQTHGVGYDFTRRLLEDNSKLSILGDGSQSKSYIYVTD
                     IIKAIRNIQLNHLKGYSYFNVSTLDYISVKEIADVTLEVLGLENVKYEFTGGNRGWKG
                     DVPVVRLNSEKIRKTGWSNEFTSREAIKKSVQSIYEDAMKNKFGWKEESIK"
     gene            6248..>7082
                     /locus_tag="PLX80_11735"
     CDS             6248..>7082
                     /locus_tag="PLX80_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQY21506.1"
                     /translation="MILSRAPVRISMGGGGTDLPSYYEKHGGFLLAASINKFVHIMLN
                     KRFEDSIRLSYSKTEIVDKVKDIEHRIFRESMKFANVKKQVEIVSIADVPSNCGLGTS
                     STFTVALLSALFSYNRDHKTLEEIAECACNIEINLLKEPIGKQDQYAATFGGFNAYTF
                     NKDGTVEVDAVKISEGAMMELQSNMFLFYLNKKRSAGDILKHQNKKTKQKNTDVLNRL
                     HKIKDIGLYTKKILEKNKIDEFGDLLHDHWLIKKGLSDKISDPLIDEVYETARKNGAI
                     GG"
BASE COUNT         2690 a          884 c         1284 g         2224 t
ORIGIN      
        1 aatttcatat ttatggtgat ggtgagaaaa gaatagaatt tgaaaaatta attattgatt
       61 acggattaga aaaatcagta ttcttaaaag ggtatactaa agatgtatta tcagttttaa
      121 gaacattcga tctatttgtt ctgacttcca gatatgaggg cattccatat gtaatattag
      181 aagctatgag tatgaatata ccaattgtaa ctaccgatgt tggtggaata agtgaagtaa
      241 taaaaaataa aatcaatgga ttacttgcag agaacggcaa tgtttctgat atttcagaaa
      301 aaattatata tataatggaa aatgatgaat taagaattca atttattgat aaagcaagaa
      361 aagattttga agaaaaattt ttaatcgaaa atactgtatc gcagattgaa aagatttacg
      421 aaatgaaata aattgatttc acaattttga aagataaaat aaaacttgta catattatct
      481 caaatcttag cttaggagga gcacaggtat tactttttga tattcttcat tacctttcaa
      541 aaaaaaatga tctggaaatt tatgttatca caatagattc cggcgagtat atagaaaaat
      601 ttaaaaaagc aggaataaaa gtttttgatt taatggaaaa aggtttggtc aatccaaaaa
      661 tttattttaa aatgaaaaaa cttttaaagt atttaaatcc tgacatagtt catactcatt
      721 taaataaagc tgatttttat ggacgaatag ctgctaaaaa aacaggtgtt aaacttattt
      781 attcgacatg tcacaattat tcaacacatc acaaaggagc agatgtaaat aaaatctcaa
      841 tttttgatta tatagataat tttgtagtca aatattcggg atcatatctg attgccatat
      901 cagaaattgt taaaaaatat ttgataaaca gggataaaag atttgatagt attactgatg
      961 tgatatataa tggtgtaaat attagtaaaa aaaattatca gttggaagaa aatgaaatcg
     1021 atttattgcg gattaaatat aatgtaagta aagacgattt cttaatttgt ctgtcaggta
     1081 gatttgataa acagaaaggt catttatttt tactggatgc actttcagaa aaaattaaag
     1141 aaagaaaaaa tataaaaata attatgcctg gtgacggtat tttaaaaaag gaagttgaaa
     1201 attatatttc aaaaaatgat ttaaatgaat ttgtgatttt gccagggttc cataaagata
     1261 ttgaaaaaat tattgaaatc agtgatctga ctatcgttcc ttcactttgg gaaggatttg
     1321 gtcttgtagt aatagagtca atggttaaga aaaaagtagt attggcttca gataccgggg
     1381 ggattcctga aattattgag catggagaaa atggatattt atttaaaaca ttgaataaat
     1441 cagatttcct tgaaaaattt aatttcatct attctgttaa tagtaattta cctgaaatac
     1501 aaactaaagc tttaaatact gttgaaatga gatttgatat aaataagaat tcggaatttt
     1561 attatttaag ttatttaagt aaatttcaat caatttctaa ataaaataat tctgaaaaaa
     1621 gtgactgaaa atttatgtcc agcttgcgga tcaagtgatg ttaaaaattg tggtcaacct
     1681 atttacagtg ataaaggatc aaagtattct gtaattgagt gtaatatctg tcagttgaac
     1741 tggtgtaatc ctatgccaac tatagatgaa cttgatgaac attatttgaa atattatgaa
     1801 gtcagacata atactgtcga aaagagtgca atgaaacttg ctataagaaa aaagatcaca
     1861 ttcagagagt atagattgaa aaaatttttc tcactaatta aaaagttttc accggtaaac
     1921 tacattcttg attttggatg tggtgaagca gaaatattat atttagcaaa aaagaaacaa
     1981 tggcaagtcc ttggagtgga ttactctaat gagttaagtg aaaaatttaa taaagagaat
     2041 ataaatttta tacaggcaaa tgatttattt tctataaata ttgaaaaaag cagtgtcgga
     2101 tgtatctcag caaaacacac tattgaacat ctgacaaacc ttgagttttt ttttgattca
     2161 acaagagata ttttacataa agatggaata cttgcaatca agacaccgtc ctcaacaagt
     2221 ctaagagcaa aaagaggtct ttcaggatgg catcatgtaa atccgcctga gcattgctgg
     2281 ggatttaatc ttaacaattt tggtaaactt gtaagcagaa atgggtttga agttgtttat
     2341 ttaaaagacg gtatattggt aaatgaatta acatgtattg cgagaaaaat ttaaccgaaa
     2401 aaataaacta atttagataa gacaatttcc atttttaaaa cttaaaatta aattttattt
     2461 atcatgaata aattattatc taaaatcttg ttcaagacag tatttgtaat cttaccattg
     2521 atattagttg taattgtttc aaatataata ctttccaata actctgagga agttaaaaat
     2581 gacaaattta aatggcagct tacatttgaa gataactttg attcatttga cagatcaaag
     2641 tggcagacta tgtttgatat gaataccaga acgatatggt caaataaaga acttcaatgg
     2701 tacaaagatg aaaacgtatt tgctgataat ggagtcttaa aaataatcac aaaaaaagaa
     2761 tcaatttttg gtaaagatgc agaaagtgaa aagcagtttg agttcacatc cggaatgata
     2821 aacactgcaa gaacatttac ccagagttat ggtaagtggg aaatcaaagt aaaatttcca
     2881 tacaaaaaag gatattggcc tgcattctgg ttagtcgcaa aggaaagacc cggattaccg
     2941 gaaattgatg tatttgaata ttttggaaga aaagaaaatt caatgtcatc atctttacat
     3001 tggggaatta attatccaag ttcagagaaa gataattcag caccgtttta ttatgttaag
     3061 tcaaacgaaa tcacaggtga cttttcagat aactggatga tctggtcatt agaatgtttt
     3121 cctgataaaa tactctggaa attgaatgat aaaattgttt tcgaatctac acagggaatt
     3181 ccaacaggtc ctatgtatat aattgcaaat gtagcaatta aagcctggga tgaaaataat
     3241 ggtgaagtag atagatcaga tgatccttat gtaatggaga ttgattatat aaaagcgtat
     3301 aagataatac cagaatagag gactattatt taattaattt taaaaaataa gatactataa
     3361 aattaatttc ttttttcaat tttaacaatt tcagaaataa atttatttaa gtacgttctc
     3421 ctttatgaat tttaaattta aagcaaaaat acaaaatctt ttctcagtat taccaaaggg
     3481 ggagaatata aattattttt ttcagaagca tgtaacaaag tcacttccaa tatctgatga
     3541 agattttaaa attaaactcg aaactgtaaa actgcatttt gacaactatg taaaatattc
     3601 cggaaacatt gattttaaaa gaagccgata ttatgaattt ggttcgggat atgatcttgt
     3661 aattccaatt ggaatcagat cactcggatt tgatgagtta acttgtatag atattcgtga
     3721 actagttttt cccgaattga taaatgatac aataaacaga tttaataaaa cggccgggaa
     3781 aatttcagat gagttttgtc tgtttgaaaa tgtacctact gtaaccggta gcaattataa
     3841 aaatgtatta tctgagtatt ttaatattaa ttatattgca ccattagatg ccagaaatac
     3901 tggtatagaa tcggacagca ttgattttat cctttcaaat gctaccctag aacatattcc
     3961 tgaaattcat attgaagaca ttttgaaaga atgcaacaga attttaaaac caggcggaat
     4021 catgtccaat gcaattgact acagagatca ttggtcattt tttgataata atataagtat
     4081 atataattat ctgctatatt ctgaaaatga atggaaaaaa ttaaatcctt ccataatgta
     4141 tcaaaacaga atgagacata aggatcatat tcagaagata aagaatgcag gatttgaaat
     4201 aatatcggaa aagttggatc ttgcggatga ttcagaaatt gaaaaattga aaactattaa
     4261 tctaaatgac catttcacga ataattatac ttttgaggaa atagcaatca aatcaagtat
     4321 gttgatcctc cgaaaaaaca attaattcaa aaaaatttct aacttttatc ttaaatccct
     4381 acttgataac ttccacgttt aataatttat gagttcaaat tccgtgccta gggttagtat
     4441 tattatccct acttataaca gagtggatat gctcgagaaa tcaatcgaaa gtgtattctc
     4501 tcaatctttt acggactggg aactcattat tattgatgat gcatctactg acggaactga
     4561 agaaagaatg actaagcttt ctgaaaggga accggcaata aattacatga ggatcccaag
     4621 gattacagga aaaggaattt ctgaatatct gaatattggc cttagaaatt ccagaggtga
     4681 atatattgca agaatagatg atgatgactt ttggtgtcat aaagataagc tgaaaatgca
     4741 agtggaattt ttagataaaa atccggagta tgttgtagtc ggtggcggtg tgatcttagt
     4801 tgataaagat ggtaaagaac tttttaaata tctgaaaaag gaaactgacg aagagatcag
     4861 aagctttgca cttttttcaa atccatttac acatgctact gtaatgttta gaaaagaaac
     4921 cgccatgaag cttggcggat ataaaaatct ggaacatgta gaagatatgg aactttggct
     4981 gcgaatgggg atggaaggga agctttataa tttcaaagaa tatttcatca catatatgac
     5041 agccggtcag aataagtcat ttactcaaca gagggaaaat tccagaacgg ttgtaaatgt
     5101 ggtaaagatg cacggaaaaa attatccgaa ttattacaaa gcatatttgc tgaattatac
     5161 acaatatatg tattcctttt taccggtatt ttttaaaaaa cggcttcagt catttatgta
     5221 ttatttgaaa cgaaagaatt tttaatatta atttttatta agtaatattg ggtaagaaat
     5281 attttatagc aggcggagcg ggatttgtag gcagccatct atgcagagac attttaaaaa
     5341 acgaacctca tgctgagatc actatctatg ataatttcag ctcaggtcag atgtggcata
     5401 tagatgattt taaagttaag ataaaaatta tcaaagctga tattaaagaa aaagatacac
     5461 tgaatgatgc aatgaaagat caggaagttg tttatcattt cgcatcaaac cctgatattg
     5521 caaaagctat gactcagccg gatattgatt tctgggaagg gacatatctt acaaataata
     5581 tcatcgaagc aatgagaatc aacagcgtaa agaatttaat ttatgcttcc ggaagcggtg
     5641 tgtatggaga tgttggtttt actgaaactg ctgaggactt ttcaccgcag cttccgatct
     5701 ctacttacgg atcgagtaaa cttgcatgcg aggcaatgat cggttcttat tgttatatgt
     5761 ttgatatgaa tgctgctgct ttcagatttg caaatgtagt cggaccaaat cagactcacg
     5821 gagttggtta tgactttacg agaaggctgc ttgaagacaa cagtaaactt tccatacttg
     5881 gtgacggatc tcagagtaag tcttatattt atgttacaga tattattaaa gcaatcagaa
     5941 atattcagct caatcatctg aaaggctatt cttattttaa tgtatcgaca cttgattata
     6001 tcagtgtgaa agagatcgca gatgtgacac ttgaagtatt gggactggaa aatgtgaagt
     6061 atgaattcac aggcggcaac aggggatgga aaggtgatgt accggtagtc agacttaatt
     6121 cggaaaaaat cagaaagaca ggctggtcaa atgaatttac atctcgggaa gcaattaaaa
     6181 aatcagttca gtctatatat gaggatgcaa tgaaaaataa attcggatgg aaagaggaga
     6241 gtattaaatg atcttatcga gagcacctgt gagaatttca atgggcggcg gcggaacaga
     6301 ccttccgtca tattatgaaa aacacggtgg atttttactg gcagcaagca taaataaatt
     6361 tgtgcatata atgcttaata aaagatttga ggattcgatc agactttcat attcaaagac
     6421 agagattgtt gataaggtaa aagatattga gcatagaata ttcagagagt caatgaaatt
     6481 tgcaaatgta aaaaagcagg tagagatagt ttctattgct gatgtccctt caaactgcgg
     6541 actgggtaca tcttctacat ttactgttgc gttacttagt gcattatttt catataacag
     6601 agatcataag acgcttgaag agattgcaga gtgtgcatgt aatattgaaa ttaatttgct
     6661 gaaagagccg atcggaaagc aggatcagta tgctgctaca ttcggcggat tcaatgcata
     6721 tacttttaat aaggatggga cagtagaagt cgatgctgtt aaaatttccg aaggcgcaat
     6781 gatggagctg cagagtaata tgttcttatt ttatcttaat aaaaaaagat cggccggaga
     6841 catactaaag catcagaata aaaagacgaa acaaaaaaat actgacgttt taaacaggct
     6901 tcataaaatt aaagacattg gactttatac aaaaaagata ttagagaaaa acaagatcga
     6961 tgaattcgga gatttacttc atgaccattg gcttattaag aaaggtttat ctgataaaat
     7021 ttcagatcca ttaatcgatg aagtatatga aacagccaga aaaaatggag caataggtgg
     7081 aa
//
LOCUS       DAORVQ010000171         7061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6485_length_7061_cov_5.012418, whole genome shotgun sequence.
ACCESSION   DAORVQ010000171 DAORVQ010000000
VERSION     DAORVQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6485_length_7061_cov_5.012418"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1619)
                     /locus_tag="PLX80_11740"
     CDS             complement(168..1619)
                     /locus_tag="PLX80_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21507.1"
                     /translation="MFNSILIETVKSFSKEEMKEFDLFIRSPFFNSNQSVIKLYEQIR
                     KLFPEYDDKNVDKKLLYFNAFGKIDYNDGFMRMNVSRLLDLAKKFLIQKNLQRSDFKY
                     EEILLDELNFRELNSIMLKSINVLNLKLEKQKAKDADTYFEKFKLEYFKNDVKARDTK
                     FINYKDTLDKDLMLEQKNLNIFYFISSLKFFQYFLNQKNFVVNTEGYPDFMNSIIDFL
                     KSNKEYLDIPALKVYYYMVLLISSGGDNYFYELKKFLFEDAESLCEVEKYNMITVLRN
                     YAQQRIQEDKKEFIDLSFDILKYSIDNNLITSSSGVKYMSETRFMNIVWTGLTLKKFD
                     YIEEFIKKFINRIEPDKREYVLAYNIARLEFEKGNFSEALEILGDSGTVKNVYYKAAI
                     KQLTLMIYYEMNWFVPAGDLLNAYRYFIKTDKILPAIYKTSCNTFINYYSKLLKINDN
                     IVKDKVDISSLISELELTSHVWLLNKARELLES"
     gene            2183..3778
                     /locus_tag="PLX80_11745"
     CDS             2183..3778
                     /locus_tag="PLX80_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21508.1"
                     /translation="MKLIKIICSLYLTLFLINQADSQVTEEWAAIFNGSGNTNDAASD
                     IIIDVSGNIIVTGRSVTSSPTANFNTVKYNSAGVQQWSVIYNGPSNDYDEPFGLVADA
                     SGNIYVTGMSYSAITNYDIVLVKYNSSGIEQWVRSWSNAGSLSDIPNDITIDVTGNIY
                     ITGESSITTSSNYITLKYNSAGSLQWAKQYNAGTAHGIEADAAGNVYVTGQGEFTTSG
                     SDFLTIKYNSVGDSLWVRRYGSVDFNERPTAFALDQNGNCYITGTVTPGNTSGFLTVK
                     YNTAGVQQWVAGNDSANKPEDVEVDILGNVYVTGSSFAGTSANCATIKFNSSGVRQWI
                     SIYNYLPEALGDYANDLELDAAGNVYITGSSQTQGGTNRDVLTVKYNNDGVEKWVARY
                     DAANSEEAFALALDAPGNVYIAGYRTITPNTDFLTIKYSQTVGINQISSEIPDGFSLS
                     QNYPNPFNPVTNLEFAISDLGFVTLKIYDINGREISTLVNEKMSAGSYIVQWDASNYS
                     SGSYLYKLDSEGFTETKKMILMK"
     gene            3906..4157
                     /locus_tag="PLX80_11750"
     CDS             3906..4157
                     /locus_tag="PLX80_11750"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21509.1"
                     /translation="MTDSAGNIYVTGSTSRGYDLPDMVTIKYTVRAVTNYELRITNYE
                     LQVTNIEFQKTIRNKTEYKAAAGFCLRFPLYCRIFTDYL"
     gene            4346..6823
                     /locus_tag="PLX80_11755"
     CDS             4346..6823
                     /locus_tag="PLX80_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY21510.1"
                     /translation="MKQFIFLLICVVCLLQLSIQKSYSQMTWNQACTFEGTSSSYVAV
                     PHSSSLNINGNFTVEAWIMPINVTSPSAQIILQKRAVGNNSGYTLYLSSGRVAVRTNS
                     STRLIGNTVIPNNQWTHIAGSYEFSSGIFRVFINGIQDSTSTVAGAAPISNTDSLLIG
                     VGSNSPFNGLLDEVRIWNTTYTNSVVPVVMRMSLGTNSGIFSNLVLSLPFQNKNSTGV
                     LFNLSDMSGNNNNGINNGVSAFNVSSPSNTISLNECLELDGNGDYLSGPDNSTVSPTS
                     EITIEAWIYPESFNANTNILSTIVHKGNSNGSVTDYNMSVNNKKFNVSVNETSIFSLS
                     TSGEFFPLNKWTHVAFSYSGATGFTQFFLNGEIRWDDTNFVGNIHDNTDSLYIGGTNL
                     LQCFDGLLDEVRITKEVLTYNTLSNQVFNSVNESNDPTASNVSYNFDGHLESNSDTGP
                     RLNFRNSARFSLNSYFNNKPVSPMSSSVSDNFSKGFYLSNPLVRVPNSGTSGFMNSDT
                     IDVPVSETISDINIFVALNHTDEDNLVLTLISPSGTSVTLYSTSSLTGTSDNLVTIFN
                     DQANLSLVSNTYVMFTPTIKPLNNLNSALSGTNTLGKWKLRIQDAAASDTGILYGWGI
                     QFNNQVKRKSILSLTSIIQGFYNPAANLMVPDSMRVFIRNNVSPYSILDSSKAVLNSS
                     GKANFELNNVPDGLPVYIQLKHRNSVETWSRVPGSSTFSILFSTHFTPFNSFLEYDFT
                     TSSNKAFGNNQIQVDTGPNKFAVFGGDVNQDGTVDLADGSLIDNDSFNFLSGYLKTDL
                     NGDNFTDISDYTIAVNNESNFVGKITP"
BASE COUNT         2375 a         1162 c         1167 g         2357 t
ORIGIN      
        1 aagatccaag atcagaatgt aactgtttgg ttttattatc ggagttttct tgcttagtat
       61 ggtgacctta taattttttt aaaatacttc attttcattt cagaaaatgt tttatatcag
      121 acaagcatat atattgatta tataacttgg aaattttaaa tgtattttca actttcaaga
      181 agttcacgtg ctttattaag aagccagaca tgagaagtta attccagttc tgaaatcaaa
      241 ctggaaatat caaccttatc ttttacaatg ttatcattta tttttaaaag tttgctgtaa
      301 taatttatga aagtattgca tgaggtttta taaatcgcgg gaagaatttt atcggtttta
      361 ataaaatatc tgtatgcatt gagcagatcg cctgccggta caaaccaatt catttcataa
      421 tagatcatca gtgtcaactg ctttatagca gctttataat acacgttttt aactgtgccg
      481 ctgtcaccga gtatttctag tgcttcagaa aagttgcctt tttcaaattc cagtctggca
      541 atgttgtatg ctaatacata ttctctttta tccggttcta ttcggttgat aaattttttt
      601 atgaattcct caatgtaatc aaattttttt aatgtcaggc ctgtccatac tatattcata
      661 aatctagttt cactcatata ttttacaccg gaagaagaag taattaaatt attatcgata
      721 gagtatttca gaatatcaaa tgacaaatca ataaattcct ttttgtcttc ttggattctt
      781 tgctgggcat aattccgtaa gacagtaatc atattatatt tttccacttc gcagagactt
      841 tctgcatctt caaaaagaaa ttttttaagt tcataaaaat aattatctcc cccgctggaa
      901 atcagcaaaa ccatataata gtaaaccttt aatgcaggga tatccaaata ctctttattg
      961 gattttaaaa aatctataat actattcata aagtcaggat atccctcggt attgactaca
     1021 aaattttttt gattcaggaa atactgaaag aatttcaatg agctgatgaa atagaaaata
     1081 ttcagatttt tctgttcaag catcagatct ttatccaaag tatctttata attaatgaat
     1141 tttgtatctc tggctttgac atcattttta aaatattcca gtttgaattt ttcaaaatat
     1201 gtatctgcat ccttagcttt ttgcttttct aattttaaat taagtacgtt aattgacttc
     1261 aacataatac tgttcaattc ccggaaatta agctcatcaa gtaggatttc ctcatactta
     1321 aaatcacttc tttgcagatt tttctggatc aaaaatttct ttgctaaatc cagaagccga
     1381 gaaacattca tccgcataaa accatcattg taatcaatct ttccaaaagc attaaaatac
     1441 aataactttt tatcgacgtt tttatcatcg tattccggga aaagttttct aatctgttca
     1501 tacaatttaa ttacactctg attagaatta aaaaatggag acctgataaa caaatcgaat
     1561 tctttcatct cttctttact gaaagatttt accgtttcga ttaaaattga attaaacata
     1621 atttagagtt tatgaattta tggttgcaat cagtgtataa aattgtccaa aattctatta
     1681 aaaacaatca aattagaaat attatgaatt ttattctgat acaatttaga ataactctct
     1741 ttgatttagc aaatcagctg atatattttt gtatgtgaag aaagtagaaa taataccgga
     1801 aaattttaca gcagcaaaag gagatacaaa aggtgaagtt aatcttcagt gggattctgt
     1861 tggaatactg tctcatatat atgattgaaa ttgctgttct aaatattcaa aagtaattaa
     1921 atggaaaatt cttgatataa ttttcgattc ccgatatact gtaaaaaatc tcagatcaaa
     1981 taaaaattat tatttccgtg tactttcaat taatagtgaa ggacaaaaag aatcaagtga
     2041 gaaaataatt aagaaagcac cgtgacaatg ttcaatgttc aatgtgcagt gtttaatatt
     2101 caatgttcaa ttatcattaa aaaatttaac tgaacttaaa aaaataattt caaaataata
     2161 attaaataag aaaggattta tcatgaaatt aattaagatc atctgctcat tatacttaac
     2221 tttgttctta ataaatcaag cggattcaca ggttacagag gaatgggctg ctatatttaa
     2281 cggatccgga aataccaatg acgcagcttc tgatattatt atagatgtat ccggaaatat
     2341 tattgtaacg ggcagatcag tgacaagctc acctacagct aattttaata ctgttaaata
     2401 taattccgcc ggggttcagc aatggtctgt aatatacaat ggtccttcaa atgattatga
     2461 tgagccattt ggtctggttg cagatgcttc cggaaatatt tatgttacag gcatgtcata
     2521 tagtgcaatc acaaattatg atatagtact ggttaaatac aattcatccg ggattgaaca
     2581 gtgggttcga agctggagca atgccggaag tctttcagat attccgaatg atataaccat
     2641 tgatgttacc ggaaatattt atattacagg tgaatcctcg attaccacaa gttcaaatta
     2701 tataactctg aaatataatt cggcaggctc attacaatgg gcaaaacaat ataatgcagg
     2761 tacagcacat ggcattgaag cagatgcagc aggaaatgta tatgtaacag gtcaaggtga
     2821 gtttacaaca agcggttctg attttttgac aataaaatac aattcggttg gtgattcatt
     2881 atgggtaagg cgatatgggt cagttgactt caatgaaaga ccgacagcat ttgcattaga
     2941 tcaaaacgga aattgctaca tcaccggtac tgtgacaccc ggtaacacat caggttttct
     3001 aactgtaaaa tataatactg ccggagttca gcaatgggtt gccggcaacg actctgccaa
     3061 taagcctgaa gacgttgaag ttgatatttt aggaaatgta tatgtaacgg gcagttcatt
     3121 tgcaggcact tcagcaaatt gcgcaacaat aaaattcaat tcatcaggag ttcgtcagtg
     3181 gatctcaatc tataattatt tacctgaagc tcttggagac tatgcgaatg atctggaact
     3241 ggacgcagct ggaaatgtct atattacagg aagcagtcag acccagggag gtacaaaccg
     3301 cgatgtactc accgttaaat ataataacga cggtgtagaa aaatgggtgg cacgatatga
     3361 tgcagcaaat tcagaggaag catttgctct tgcactagat gcaccaggca atgtatatat
     3421 agccggttac cgcacaatca cacccaacac tgatttttta acaataaaat attcgcaaac
     3481 agtcggaata aatcaaatct catcagaaat acctgatgga ttttcacttt cacaaaatta
     3541 tcctaatcca ttcaaccctg ttacgaatct ggaatttgca atttcggatt tgggatttgt
     3601 tactttaaaa atttatgata taaatggacg tgaaatatca acactcgtta atgaaaaaat
     3661 gtctgccgga agctacattg ttcaatggga tgcatctaat tattcaagtg gttcatattt
     3721 atataaactc gattctgaag gtttcacaga aacaaaaaaa atgatcctga tgaaataaac
     3781 ttttgaaaag attctgtatt caatttattg tgtatgtaaa tgcagtatcc aattcgcaga
     3841 taacacagga ctaggtaaaa agctacagca gtattcaact gaattcacag attgcttttg
     3901 acgctgtgac cgacagcgca ggaaatatat atgttacagg ttctacttca aggggttatg
     3961 atcttcctga catggttaca ataaaataca ccgtaagagc agttacgaat tacgagttac
     4021 gaattacgaa ttacgagtta caagttacga acatagagtt tcaaaagaca attagaaata
     4081 aaactgaata taaagcagcc gcaggttttt gcctgcggtt tcctttatat tgcagaatat
     4141 ttaccgatta tctttgatga caagtatcca tttttcatac cattaatttt tatatgtatt
     4201 tttttttctc acatcatagt ctaacattgt aacgttatca atattattca tttggtgcta
     4261 agtttatcct tgttaattca ttgagatgaa attaacttat ttctacccaa tatatttttc
     4321 aaataacttt tacaaggaga taaaaatgaa acaatttata tttttattaa tctgcgttgt
     4381 ttgtcttctt cagctcagta ttcaaaaatc ctattcacag atgacttgga atcaggcttg
     4441 tacttttgaa ggaacaagtt caagttatgt tgctgtacct cattcatctt ctttgaatat
     4501 aaacgggaat tttacggttg aagcctggat catgccaata aatgtaactt caccttctgc
     4561 gcagatcatt cttcagaaaa gagctgttgg aaataattca ggatacacgc tttatcttag
     4621 cagcggaaga gttgcagtca gaacaaattc atccacaaga ctaatcggaa atactgtaat
     4681 accaaataat caatggacac atatcgcagg atcttatgaa ttttcatctg gtattttcag
     4741 agtttttata aacggaattc aggattcgac ttcaacagtc gcaggagctg cacctatatc
     4801 aaatacagat tcattgctga ttggggtagg gagtaattcg ccatttaatg gacttcttga
     4861 tgaagtacgt atttggaata ctacatacac caattctgtc gttcctgttg taatgagaat
     4921 gtcactcggt acaaattcag ggattttttc taatctagta ttgtctttgc cttttcagaa
     4981 taaaaattca acaggcgtat tgtttaacct ttctgacatg agcggaaata ataataacgg
     5041 aatcaacaat ggagttagcg catttaatgt cagttcacca agcaatacta tttctttaaa
     5101 tgaatgcctg gaattagacg gcaacggcga ttatctttca ggaccggata attctacagt
     5161 cagtccgact tcagaaataa ctattgaagc atggatctat cctgaatcat ttaacgctaa
     5221 cacaaatatt ctaagtacca ttgttcataa aggaaattcg aatggaagtg taactgatta
     5281 taatatgtcg gttaacaata aaaaatttaa tgtttcagtt aatgaaactt ccatttttag
     5341 tttaagcact tcaggagaat tctttccttt aaataaatgg acgcatgttg cattcagtta
     5401 ctccggagca acgggattta cgcagttttt tcttaatggc gaaattaggt gggatgacac
     5461 aaattttgtt ggaaatattc atgataatac ggattcactt tatatcggag gaaccaattt
     5521 gcttcaatgc tttgacggac ttcttgatga agtaagaatt acaaaagaag tattaacata
     5581 caacaccctc tcaaatcagg tctttaattc agtaaatgaa tcaaacgacc ctactgcatc
     5641 aaatgtgtca tataattttg acggtcatct tgaatcaaac agtgataccg gaccgagact
     5701 taatttcaga aatagtgcaa ggttcagtct aaattcttat tttaacaata aaccggtttc
     5761 acctatgtca agctcagtaa gtgataattt ttccaaagga ttttatctca gcaatcctct
     5821 tgtcagagta ccaaattcag gaacgagcgg attcatgaat tctgatacta tagatgtacc
     5881 tgtaagcgaa acaatttctg acattaatat atttgtagcg cttaatcata cggatgaaga
     5941 taatcttgtc cttactctga tctctccttc aggcacttct gttacacttt acagtacatc
     6001 atctttgaca ggaacttctg ataatttagt aacaatattt aatgaccagg caaatttatc
     6061 tttggtcagt aatacttatg taatgtttac tccgactatt aaacccttaa acaatttgaa
     6121 ctcagctctg tcaggtacta atactttagg gaaatggaag cttagaattc aggatgcggc
     6181 tgcttctgat actggaattc tttatggctg gggaattcag tttaataatc aggtaaaaag
     6241 aaaaagtatt ctttccctca cttccattat acagggattt tacaatcctg ctgcaaactt
     6301 aatggtgcct gattcaatga gagtctttat aagaaataat gtctctcctt attcgatctt
     6361 agattcatca aaagctgttt taaatagttc aggaaaagcg aattttgaat tgaataatgt
     6421 tccggacgga ctacctgttt atattcagct gaaacacaga aactctgttg agacatggag
     6481 tagggttccg ggttcatcaa cattttccat actcttcagc actcatttta cccccttcaa
     6541 ttcttttctg gaatatgact ttaccacttc ttcgaacaaa gcattcggaa acaaccagat
     6601 tcaggttgat acaggaccga ataaatttgc agtattcgga ggtgatgtaa atcaggacgg
     6661 aacagttgac cttgcggacg gtagtcttat tgataatgat tcatttaatt ttttatcagg
     6721 ctatctgaag acagatctga acggagataa ttttaccgat atatctgatt atacaattgc
     6781 cgtaaataat gaatccaatt ttgttgggaa gataactcca taagagcaat taagaattaa
     6841 gaattaagaa ttaaaaatta ctaaatacga attacacgca aaatggtaag aaaatttaag
     6901 ttgtaataga atatttggaa agaagcctgt tgagaaatct acctgctctt tgtttttgca
     6961 ttttggagct catcaaattt ttccattagc cagatcttat ttgagacatc tctttcgtta
     7021 atttgatttt tcagattatt caggtaaaaa tcatcgtatt t
//
LOCUS       DAORVQ010000172         6967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6645_length_6967_cov_6.650897, whole genome shotgun sequence.
ACCESSION   DAORVQ010000172 DAORVQ010000000
VERSION     DAORVQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6645_length_6967_cov_6.650897"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1572)
                     /locus_tag="PLX80_11760"
     CDS             complement(160..1572)
                     /locus_tag="PLX80_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQY21511.1"
                     /translation="MKLLLFNFIFIISITSVSISQQPPNNMHLLGNLNQHSLPPQFNA
                     PWSYAAIWGYQSPDNREYAVLGCALGTAFYDVTDSANIHEVGYFPNVIDTLNPDQGVL
                     WQEMKVYSHYAYVVSEADTSGVKIYDLQYLPDSVVYVKKFIAAGHRSTHSISQEGPYL
                     YLNGSNSSFGNGTAVLDLSQDPVNPVLRGKWNTRYIHDCRVLNDTIFAMNINQGDGQI
                     TVIDARNKDSLKTITQWFNLPNPAPHNNALTADRDYLYATDEVGPLPRLLKVWDIRDL
                     SNVVQVNTWQPTGITTSIVHNVEIYGDTAVIAHYTAGVRVLDISNPVMPVEIGWYDTY
                     PASNNNLYQGCWGVYKFPSGKIIASDMNTGLYVLKMGKTVGINNNVTELNEFRLEQNY
                     PNPFNPKTIINYELGNKNFVSVKVYDVLGNEVRTLVNENKPAGSYSIEFDGSDLSSGM
                     YFYKLTTDSFNEVRKMILLK"
     gene            complement(1685..3703)
                     /gene="uvrB"
                     /locus_tag="PLX80_11765"
     CDS             complement(1685..3703)
                     /gene="uvrB"
                     /locus_tag="PLX80_11765"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQY21512.1"
                     /translation="MKFKLESKYKPAGDQPAAIKELTEGIERGDKFQTFLGVTGSGKT
                     FSVSNVIENIGKPVLVMSHNKTLAAQLYGEFKRFFPNNRVEFFISYYDYYQPEAYIPS
                     SDTYIQKDLSINEEIDRLRLRATASILEGRNDVIVISSVSCIYGIGAPQEYAEQMILL
                     KVGDKISRKVLMSRLVNIHYVRNDTDFLRGTFRVRGDNIDLIPAYENNTGVRIEIFDN
                     TIEKISYINREDGNVIGNDISVVIYPAKYFLTSKEKIDDAIVDIRAELEVQIEYFKAQ
                     GKAIEAQRIEQRTNFDIEMIQEIGYCNGIENYSRHMDRREPNSRPSCLFDYFPKDFLL
                     VVDESHVTAPQIRGMYNGDRMRKKTLVEYGFRLPSALDNRPMTFEEWESMIKQAIFVS
                     ATPGDYELEKSEGVVVEQIIRPTGLLDPEIQVRPVKNQIDDLINEVRVRSKRGERVLV
                     TTLTKRMSEDLTDYLEGIGIRVKYMHSEVDTLDRVEILRSLRLGNFDVLVGVNLLREG
                     LDLPEVSLVAILDADKEGFLRSEKSLMQTAGRTARNANGLVIMYADKVTKSMDKVIKE
                     TARRREIQMQYNKDHGITPETVLKTYEEIMSATIVADSKVKYEGKKDKVEKKSTLSIL
                     TDPVSKKINSEQRKELMEQLRKEMINAAKDLEFERAAELRDEIDKLNK"
     gene            complement(3952..4221)
                     /locus_tag="PLX80_11770"
     CDS             complement(3952..4221)
                     /locus_tag="PLX80_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HQY21513.1"
                     /translation="MSDTDIKEKIIFDGENDVLHKLILFNSNHIWDEVILQLQKATGF
                     DILQCEQIAVIAHTKGKAVVCSGDIPKLNGINSVLKEIKLVTEIE"
     gene            complement(4234..5484)
                     /locus_tag="PLX80_11775"
     CDS             complement(4234..5484)
                     /locus_tag="PLX80_11775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HQY21514.1"
                     /translation="MLYKQYRKSFNKFIRGLVFSALIVIVSTGNSFSKDNLSILKENI
                     DLILSGVSGNVSVQVASADKYDLIYEYNPQLKMIPASITKMITAAAALEYLGTDHNFR
                     TVLYSDDSNISDGIVNGNIYLKGFGDPDLNTNDLNYLAKLFAAKNITEVTGNLVFDES
                     YLDNQYYGLSNFYQGDTKSNYWPYVNALCLNKNHGTTDPSSTAGNILADALIYENVKL
                     GGIVISGVTPTGAKEIADVSHSMYNVLSQMNKASDNHSAITVFKVIGAAYDSPPGSLD
                     KGESAVIEFLTSMGNTRSNFEILEGSGLTRYNTVNSDLYIRLLKYMYDDLKGFDYFYN
                     SLAVSGVDGTLRNRMIGTEAEGNIHAKTGTLNSVSALSGYAVTRDNELLLFYIAMNGF
                     GGGNNGPRNIQDQVCEQLCMYSRN"
     gene            complement(5474..5920)
                     /locus_tag="PLX80_11780"
     CDS             complement(5474..5920)
                     /locus_tag="PLX80_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQY21515.1"
                     /translation="MELKKDRSIGVIIFHLNNGEPEFLIIKHRQGHWSFAKGHKEKEE
                     SDYQTAIRELKEETGIKDANFISDKIELSEMYKFNSANNFKIEKSVDYFIAEVPTNEV
                     NIDNMEILEYQWRNLCSGLELVTFAESKNILNKAYEIIINKNKNVI"
     gene            complement(5963..6739)
                     /gene="mazG"
                     /locus_tag="PLX80_11785"
     CDS             complement(5963..6739)
                     /gene="mazG"
                     /locus_tag="PLX80_11785"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQY21516.1"
                     /translation="MKKTNTTLTDEFVEFVEIVKRLRVDCPWDKIQTHLSLRRCLLEE
                     TYEVLEAIDENKPVELKKELGDLLLQVVFHSNIAEEENEFTLKEVIESETEKLIARHP
                     HVFGDVKVKDSDEVKRNWELLKKNEGRKSVIDGIPEQLPALLKAYRIQEKASKVGFDW
                     KEEEPVFDKIREELEELKINVNSGKDQKEIEDEFGDVLFSLVNFARYHKINPEDALRK
                     TVSKFKKRFNKIEDFANENDVELEAMTLEEMDAVWNKAKE"
BASE COUNT         1996 a         1405 c         1088 g         2478 t
ORIGIN      
        1 tttacaattt ctcaagaatt cttaacggca atgtattaca ggcgggattt tttttgtaaa
       61 atacttctaa cgttaataaa aaaaagagct tcaggaattt tcccgaagct cattttttat
      121 aaacttttta aaatgaaagt ttttgaaata aaaatttagt tacttcaata aaatcatttt
      181 ccttacttca ttaaaactat ctgttgtcag tttataaaaa tacataccac tcgacagatc
      241 acttccgtcg aattcaattg aataacttcc ggcaggtttg ttttcattta ccaaagttct
      301 gacttcatta cccagcacat catatacttt cactgaaaca aaattcttat ttcccaattc
      361 gtaatttata attgttttcg ggttgaatgg atttggatag ttctgttcaa gtctgaattc
      421 atttaactca gtgacattat tatttatgcc tacagttttt cccattttta aaacatacaa
      481 acccgtattc atatcagatg caatgatctt tccggatgga aatttgtaaa ctccccagca
      541 gccctgatat aaattattgt tactggccgg ataagtatcg taccagccaa tctcaacagg
      601 cattaccgga tttgaaatat caagcactct cactcctgct gtataatgag ctatcactgc
      661 agtatctccg tagatctcta cattgtgaac tatggaagtg gtaataccgg tcggctgcca
      721 tgtattaacc tgtacaacat ttgacagatc tctgatatcc cagactttaa gtaatctggg
      781 aagcggtcct acttcatctg tggcatataa ataatctctg tcagcagtaa gagcattatt
      841 gtgaggagcc ggattcggaa gattaaacca ttgagttatt gttttcagac tatccttgtt
      901 gcgtgcatca atcaccgtga tctgtccgtc accctgattt atattcattg caaaaatagt
      961 atcatttaaa actctgcagt catggatata tctggtattc catttacccc tgagcacagg
     1021 atttacagga tcctgtgaaa gatcaagtac tgcagtaccg ttgccgaatg aagaatttga
     1081 accgttgaga tataagtagg gtccttcctg tgaaatagaa tgagtacttc tgtgacctgc
     1141 agcaataaat tttttcacat atactacaga atcaggtaaa tactgcagat cataaatttt
     1201 tactccgctt gtatcagctt cagatactac ataagcataa tgagaatata ctttcatttc
     1261 ctgccataaa actccctgat caggatttaa tgtgtctata acattaggaa agtatcctac
     1321 ttcatgaata tttgcagaat cagtaacatc ataaaaagca gttccaagag cacacccaag
     1381 cacagcatat tctctgttat cgggagattg atatccccaa attgcagcat aactccatgg
     1441 agcattaaac tgcggaggga ggctgtgttg atttaaattt ccaagaagat gcatgttgtt
     1501 tggaggttgc tgggaaattg aaacagatgt gatggaaata ataaagataa aattgaaaag
     1561 tagtaatttc ataatcatag tctatttttt cgtttggttt ttagtaagtt atttcgatgg
     1621 aaatgtgtat aagtttcaac atagaatata gaacattgaa cattgaacct agaacattga
     1681 acatttactt gttgagttta tcaatctcat ctctcagctc agcagctctt tcaaactcca
     1741 gatccttcgc agcatttatc atttcttttc ttaactgttc cataagctct ttcctctgtt
     1801 cggaatttat ttttttgctt acaggatccg taagtatact aagagtagat ttcttttcta
     1861 ctttatcttt tttgccttcg tattttactt tggaatctgc tactatagtc gctgacatta
     1921 tttcctcata agttttcaaa actgtctcag gagtaatgcc gtgatcttta ttatactgca
     1981 tttgaatttc ccttcttcgt gcagtttctt ttattacctt gtccatcgat ttagtaactt
     2041 tgtccgcata cattatcaca agtccgtttg catttcgggc agtccttcct gcagtctgca
     2101 tcagcgattt ttccgatctg agaaatcctt ctttatcagc atcgagtatt gcaactaatg
     2161 aaacctcggg aagatcgagt ccttctctca aaagatttac tccgaccagt acatcaaaat
     2221 ttccaagtct cagacttctt aatatttcca cacggtcaag tgtatccact tccgaatgca
     2281 tgtacttcac tctgatacct attccttcga gataatccgt aagatcttcg ctcattcttt
     2341 tcgtaagagt agtaacgaga actctctccc ctcttttact tcttactctc acttcattta
     2401 tcagatcatc gatctgattt ttcacaggtc tgacctgaat ctcaggatcc agaagtcccg
     2461 tcggtctgat gatctgttcg actacgacac cttcactttt ttccagttca taatctccgg
     2521 gagttgcact cacaaagatc gcctgcttta tcatcgattc ccattcttcg aatgtcattg
     2581 gtctgttgtc aagtgctgac ggcagacgga atccgtattc cactaaagtt tttttacgca
     2641 tcctgtcgcc gttgtacatt cctctgatct gcggagctgt tacatgtgat tcatccacta
     2701 ccaataaaaa atcttttggg aaataatcaa acaaacagga tggtctggaa ttcggctccc
     2761 gtctgtccat gtgtctggaa taattttcaa taccgttgca gtacccgatc tcctgtatca
     2821 tctctatgtc aaagttagtc cgctgctcga tcctttgtgc ttcaatcgct tttccctgag
     2881 ctttaaaata ttcgatctga acttcaagtt cagcgcggat gtcaacgatc gcatcatcga
     2941 tcttttcttt gctcgtcagg aaatatttcg caggataaat aactacactt atatcattgc
     3001 cgatcacatt cccgtcttcc ctgtttatgt aagaaatttt ttcaattgta ttatcaaata
     3061 tctctatcct cactccggta ttattttcat aagcgggaat gaggtcaatg ttgtcccctc
     3121 tcaccctgaa agtcccccgt agaaaatctg tgtcattcct gacgtagtga atgttcacaa
     3181 gtctcgacat caaaactttt ctcgaaattt tatctccaac ctttagcagg atcatttgtt
     3241 ctgcatattc ctgcggagca cctataccgt aaatgcagct cacggatgat atgactataa
     3301 catcatttct tccttcaagt atagacgcag tagctctgag cctgagtctg tcgatctctt
     3361 catttattga aaggtctttt tgaatgtatg tatcggatga aggtatgtaa gcttcgggct
     3421 ggtaataatc ataatatgaa ataaaaaact caactctgtt attgggaaaa aatcttttga
     3481 actcaccata aagctgtgct gcaagagttt tattgtgtga cattacaagc accggctttc
     3541 ctatattctc aattacatta gatacagaaa aagtttttcc cgaacctgtg acaccgagga
     3601 aggtctgaaa tttatcacct ctctcgatac cttcagtcag ttcctttata gctgccggct
     3661 ggtctcccgc aggtttatat tttgattcta atttaaattt caatttcgtt aaaagtttgt
     3721 tgagttttaa aatctgattt ttggatttga aagaccgttt agatttaatt tactgtaatt
     3781 gctccttccc ttttttaagt gggatatact ttcaaattat tcagaatcgt gctattaaat
     3841 atttagctaa atatctcaac aagattcact gaaaattttc tgaccatttt gcgattaaaa
     3901 aagatcgtat tagattaatt cttaatcctt aattcttaat ttttaattgt actattctat
     3961 ctctgtaacg agtttgattt ccttaagcac agaatttatc ccgttaagtt ttggaatgtc
     4021 acctgagcat acaacagctt ttcctttggt gtgggcaatg acagcaatct gctcacattg
     4081 gagaatgtca aatccagtag ctttttgcaa ctgcaatatt acttcatccc atatatgatt
     4141 ggaattgaaa aggatgagtt tatgcagtac atcattctct ccgtcaaaaa taattttttc
     4201 tttaatatca gtatcagaca ttttaatata aaattaattt ctggaataca tacaaagctg
     4261 ttcacatacc tgatcctgta tatttcttgg tccattattt cctcctccga atccgttcat
     4321 cgcaatataa aaaagcaaaa gctcattatc tctggtaacg gcatacccgg aaagtgcgct
     4381 tactgaattc agggttcccg tttttgcatg aatatttcct tcagcctcag ttcctatcat
     4441 gcggtttctg agagttccat ctacccctga tacagcaagt gaattataga aataatcaaa
     4501 ccctttcaga tcatcataca tatatttcag aagtctgata taaagatctg aatttactgt
     4561 attatacctt gttaaacctg atccttcaag tatctcaaaa ttgcttcttg tatttcccat
     4621 tgaagtcaaa aattctatta ctgctgattc acctttatca agtgatcccg gaggagaatc
     4681 ataagctgca ccgatcactt taaatactgt tattgccgag tgattgtcac tggctttatt
     4741 catttgtgac aatacattat acatggaatg agagacatca gcaatttctt tagcccctgt
     4801 aggagtgaca cctgaaatta caataccgcc gagttttaca ttttcataaa ttaaagcatc
     4861 tgcaagaata tttcctgctg tagaagaagg gtctgtagtt ccgtgattct tattcagaca
     4921 aagagcattg acgtacggcc agtaattact tttcgtatca ccctgataaa aatttgataa
     4981 accgtaatac tgattatcta aataggattc atcaaagact aaatttcctg tgacttcagt
     5041 aatgtttttt gctgcaaata atttcgcaag ataattaaga tcgtttgtat tcagatcggg
     5101 atcgccgaaa cctttcagat aaatatttcc gttaactatt ccgtcgctta tattactgtc
     5161 atcagagtat aatactgtgc ggaaattatg atcggtgccg agatattcga gtgcagctgc
     5221 tgctgtgatc atttttgtga tcgatgcagg aatcatttta agctgcggat tatattcata
     5281 tattaagtcg tacttatctg cgcttgctac ctgtacggaa acatttccgc tgacacccga
     5341 aagtatcaga tcaatgttct ctttaagtat acttaagttg tcctttgaaa atgaattccc
     5401 tgtactgaca ataacgatta acgcagaaaa tactaaccct ctaataaatt tattgaaact
     5461 tttacgatat tgtttatata acatttttgt ttttatttat aataatttcg tatgctttat
     5521 ttaaaatgtt ttttgattct gcaaatgtaa ccagttctaa tccggaacat aaattccgcc
     5581 attgatactc taatatttcc atgttgtcta tattaacttc attagttgga acttctgcaa
     5641 taaaataatc tacagacttt tcaattttga aattatttgc tgaattaaat ttatacatct
     5701 cgctcaattc aattttatct gaaataaaat ttgcatcctt tattccggtc tcttctttta
     5761 gttcgcgtat tgcagtctga taatcactct cttccttttc tttatgaccc tttgcaaatg
     5821 accagtgacc ctgtctgtgt ttaattatca gaaactcagg ttcaccatta ttcaaatgaa
     5881 aaattattac tccgatcgat ctgtccttct ttaattccat tacatttaaa cctctttaaa
     5941 ttttaaatac tttttacatt atttattctt ttgctttatt ccaaacagcg tccatttctt
     6001 caagtgtcat tgcttcaagc tcaacatcat tttcatttgc aaaatcctca attttattaa
     6061 atctcttttt gaatttgctg acagtttttc tcaaagcatc ttcaggattt attttgtgat
     6121 acctcgcaaa atttaccaga gaaaaaagta catcaccgaa ttcatcttct atttctttct
     6181 gatcttttcc tgaattcaca ttaattttaa gttcttcaag ctcttcacgt atcttgtcaa
     6241 ataccggctc ttcttccttc cagtcaaatc cgacctttga agccttctcc tgaattctgt
     6301 atgctttcag taaagcagga agctgttcag gaatgccgtc tatcacagat tttctgcctt
     6361 catttttttt gagtaactcc cagttacgct ttacttcgtc gctgtctttt actttgacat
     6421 caccgaatac atgcggatgt ctcgctatga gtttttctgt ttcagattcg atgacctctt
     6481 tgagagtaaa ctcgttttct tcttcggcaa tattagaatg gaaaacaacc tgcaaaagta
     6541 aatcgccgag ctcttttttt agttcaaccg gtttattttc gtcaatggct tcaagcactt
     6601 cataggtttc ttccaaaagg catcttctca atgacaagtg tgtctgaatt ttatcccagg
     6661 ggcagtcaac tctaagtctt tttacaattt cgacgaactc aacgaattcg tccgttaaag
     6721 tagtattggt ttttttcaaa ttttgatttc gtaaagataa aaattttaat ttgttttttc
     6781 agtgtttaaa gtattgtctg atcttaccac caagacacca agacacaaag gaaataaatt
     6841 taaaaatatt aatcttggtg acttagtggc tttgtggctg atcttaccac taagacacca
     6901 agacactaag ggaaaaagtt aaaaaatatt gaactttgtg acttagtgac ttagtgtctt
     6961 tgtggct
//
LOCUS       DAORVQ010000173         6907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6753_length_6907_cov_4.661705, whole genome shotgun sequence.
ACCESSION   DAORVQ010000173 DAORVQ010000000
VERSION     DAORVQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6753_length_6907_cov_4.661705"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..3329
                     /locus_tag="PLX80_11790"
     CDS             345..3329
                     /locus_tag="PLX80_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21517.1"
                     /translation="MKNPPSGYVTFLFTDIEGSTRLSQEYPESIQAAIEKHHLIMHDT
                     IESNNGFVFDNIGDAFCCAFESAEEAVKAAVIVQKNLASEKWNDVVIKIRIGIHCGTA
                     EWNGENYMGYITLARTARIMSAAYGDQIIISNDAFELFKNNISSEILFRDLGERRLKD
                     LIQPVRLYQIVAEGIREEFPPLKTLDARPNNLPLQLSSFIGRREELKQIKSILKGKRL
                     LTLTGPGGTGKTRLALQIAADIIDDFENGVWFIELASLIEPELLPNAIMHTLGIKDQP
                     KQKVEDTLVDYIKDKELLIILDNCEHIIKACAILSEKILQNSLRIKIISTSREALQCF
                     GEQTHKTLSLETPDPKDKESPEQLTQYEAVRLFVERALSVNKNFRVNNDNAPALAGIC
                     HKLDGIPLAIELAAVRIKILSVEKIFDRLDDRFKLLTGGQRTALPRQQTLKALIDWSY
                     DLLSEEERILWSRLSVFSGGWNLDATEEICSDISVIKKEDILDLLSNLADKSIITFNQ
                     ENHRYGMLESIKQYGHEKLILSNEYKIIQDKFLKFYLELAETANKNFRGSETAFWINV
                     INCEYKNIEKGLIRSMESTENETGARFVGAIAQYWFIRGDLSEGILWLETVIKKQPEI
                     MDSYFCRVNYFIGKFARLNGDFEKAKIFLKGSLQYWREIGNIQGITDTLNSLGVNEFD
                     QGRFEQAAEYYEENLKIYKESENKKGIAVTLNNMGNVISNLGDFTKAIKLYEECLAIR
                     RELKDELGIGITLNNLGILDFEQGEYEKAYDLLNQSLKIRLRIGDRSGVSISLLNLGH
                     TAYNQGEYLEASNFYKESLKICVDIGDKSLIADSLFNLGKVALEQDGTEQALKFIEES
                     IVISREIKSHSQTAICLYFLGRIAFLKKEYKNAREYYYESINIYCETGNKKDIALNIL
                     RLAEFHGFKGHNIQSTKLLGFIKNKYFESNKIKFPLADQIIFDKLILELKEKLNEKDF
                     LKYYEEGKLITHDQAIEIAISN"
     gene            3518..>6907
                     /locus_tag="PLX80_11795"
     CDS             3518..>6907
                     /locus_tag="PLX80_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21518.1"
                     /translation="MSSILKSKAKSTIPAKKLSQENSFNNNEWKLKTEELEKNVAEKT
                     ADLIIKNRDLEIEASLERVRAIAMEMRERGDLLDVSKILFIELKNLGFSEIRNAMINI
                     HDHENKTYLNYDYSDTLGKSISHIHYDTDPTIEKQIKQIRKGKDAFSETLFKGKELTE
                     WKRLRKIRGEKDDPRLKKISALYYYFYSIGNGSIGISTFDGLSKNKLEILKRFRNVFE
                     FAYRRFEDVSKAEAQTREAKIEASLERVRTIAMGMRKPNDLLDISEILYKELKDLGFS
                     EIRNAMINIHNDEEKTFLNYDYSDTLGKSITPLFYNIHPLVEKQIKQVRKGKDAFSET
                     SFKGKELAEWKRFRKSRGEKDDPRLNKTTSLYYYFYSIGTGSIGISTFDGLSKNKLEV
                     LKRFRNVFEFAYRRFEDVSKAEAQAREARIEIALERVRSRTMAMQRSEELSETTFILF
                     RQFLELGEAPDQITIGIIDEKEKVIDFWTTMEGQKISSMVKFPVNDTDLMKKIYLAWK
                     SQKKSISIEIKGKELHDYIEYRIRLTGVSGKNDHVHDRRYIQAAFFSKGLITISTNDP
                     RSDETMQLLERFAGVFDLTYTRFLDLKQAEAQTKEAKIELSLERVRARTMAMHSSDEL
                     QEAAVLLFQQIRELGVDTGSCGYVIWENEKKNATVWMSSPEGGIQDPFKLPHTKSKIY
                     KEIYSAKEEGKEFFITEVKGAELKKHFDYLTTVPGIGEKIIQLRKARYKFPETIAYNI
                     AFFKQGYLSFHTHEQKPDAYDIFRRFANVFEQTYTRFLDLQKAEAQAREAQVQLALER
                     VRARTLSMHDSDELEAVADILFLQLQVLGIFTYRSYIGIFRNSRLADIFGAGEIKEFT
                     KTIFDETNGLISKIFVPWRKKDPTAIINTNGNEFECIAYFSSGFVAITSPDYPSSETM
                     NLLERFVGVFNLTYTRFLDLQQSEAQAREAKIEASLERVRSRTMGMQKSDELKEVIQV
                     VYDQLVQLNIFVEHSGFIMDYKTRDDMHIWLADKYMVPFEVTIPYFDCPHWNSFVEAK
                     EKGIDFFANHLSFKEKNKFYKALFKLIPGVPEETLEYYLTCPGLAISTVLLDNVGLYI
                     ENFSGTPYSDEENKTLMRFGKVFQQTYTRFLDLQKAEAQARE"
BASE COUNT         2506 a          987 c         1391 g         2023 t
ORIGIN      
        1 aaattatttt tttggtttgg ggaaagttcg ggattttgta tcgtgtaatt taaaataaaa
       61 caatattatc cgaatatgct gaattatgaa tattcaattt ttcagtttca ttaaacccta
      121 cggattttta ttaaatcttt aaattcattt ctgattgtaa tttagtaaaa tttacaacca
      181 aaataaaata tagggtttgt attttatcaa aaaaaaaatc tccggtatta ttgattttca
      241 gactatttat taattatgaa atgtatagga tcctgtctgt gcaattattt atgacagcaa
      301 taatagtaat tcaattttac tatcgttttt agtaaattta acccatgaaa aatcctccat
      361 caggctacgt aacttttttg ttcactgata ttgaaggcag cacaaggcta tctcaggaat
      421 atccggagtc aatccaggct gcaattgaga aacatcatct gattatgcat gatactattg
      481 aatcaaataa tggatttgtg tttgacaata ttggtgatgc tttttgctgt gcatttgaaa
      541 gtgcagaaga agcagtgaaa gcagctgtga ttgttcagaa aaatcttgca agtgaaaagt
      601 ggaatgatgt agtaattaaa ataagaatcg gaattcactg cggaacagcg gaatggaacg
      661 gcgaaaatta tatgggttat attacacttg cccgtacagc gagaataatg tcggctgcat
      721 atggagatca gataattatt tcaaatgatg cttttgaatt gtttaaaaat aatatttctt
      781 cagaaatttt attcagagat ttaggtgaaa gacgattaaa agatctaatc cagccggttc
      841 gattgtatca gattgttgct gaaggtattc gtgaagaatt tccaccatta aaaacacttg
      901 atgcacgtcc taataattta ccgcttcaat tgtcgagttt tataggacga agagaagagt
      961 tgaaacagat taaaagtata ttgaaaggaa aaagactttt aactcttacc ggaccgggtg
     1021 gaacaggcaa gacacgtctg gcacttcaga ttgcagccga tattattgat gattttgaaa
     1081 acggagtttg gtttattgag ctggcttcat taatcgaacc ggagcttctt ccgaatgcaa
     1141 taatgcatac acttggaatt aaagatcagc caaaacaaaa agttgaagat acattggttg
     1201 attatattaa ggataaagaa ttattaatca tacttgataa ctgtgaacat attatcaaag
     1261 cctgtgcaat tttatcagaa aagattttgc aaaactctct aagaattaaa ataatatcaa
     1321 caagccggga agcgctccaa tgtttcggag agcagacaca taaaactttg tcacttgaga
     1381 ctcctgatcc gaaagataaa gaatctccgg agcagcttac tcaatatgaa gctgtgagat
     1441 tatttgtaga aagagcatta agtgtaaaca agaattttag agtaaataat gacaatgcgc
     1501 cagctcttgc ggggatatgt cacaagctgg atggtatccc tcttgcaatt gagctagcag
     1561 cagtgagaat aaaaatatta tccgtagaaa aaatatttga tagattggat gacagattca
     1621 aattacttac cggtggacag cggactgcat taccgagaca gcagactctg aaagcattaa
     1681 tagactggag ttatgatttg ctttccgaag aagaaagaat actttggagc agattatctg
     1741 tattttccgg aggttggaat ttagacgcaa ctgaggaaat ttgttcggac atatcggtaa
     1801 tcaaaaaaga agatatatta gatctgttga gtaatttagc agataagtct ataataactt
     1861 ttaatcagga aaaccatcga tatggaatgc ttgaatccat taagcaatac ggacatgaaa
     1921 aattaatact gtcaaatgaa tataaaatta ttcaggataa gtttctaaaa ttttatttgg
     1981 aacttgctga aacagcgaat aaaaatttca gaggaagtga aactgcattt tggataaatg
     2041 ttataaattg tgaatataag aatatagaaa agggattgat caggtcaatg gaaagtactg
     2101 aaaatgaaac tggagcaaga tttgttggtg caattgcaca atattggttt atacgcgggg
     2161 atttatccga aggaatacta tggcttgaaa ctgtaataaa gaaacagcct gaaattatgg
     2221 attcttattt ctgtagagta aattacttta taggaaaatt tgcaagatta aatggagatt
     2281 ttgaaaaagc aaaaattttt ttgaaaggga gtttgcagta ttggcgggag attggaaata
     2341 tacagggaat tacagatact cttaactcat tgggggttaa tgaatttgac caggggagat
     2401 ttgagcaagc agcagaatat tatgaggaga atcttaaaat ttacaaagag agtgaaaata
     2461 aaaagggtat agctgtaact ttaaacaata tgggtaatgt aatttcaaat cttggagatt
     2521 tcactaaagc aattaaactg tatgaggagt gtctggcaat tcgacgtgag ttaaaagatg
     2581 aacttggtat tggcattact ctcaacaatc tgggcattct ggattttgaa caaggagaat
     2641 atgaaaaggc atatgattta cttaaccaaa gtttgaagat cagacttaga attggagata
     2701 gaagcggtgt tagcatttca ttactgaacc tgggacatac tgcttataat cagggagaat
     2761 atttggaagc atctaatttc tataaagaga gtctgaaaat atgtgtagat ataggagata
     2821 aatctcttat tgcagattct ttgttcaatt taggaaaagt agcactcgaa caagatggca
     2881 cagagcaggc attaaaattt attgaggaaa gtatagtaat cagccgtgag ataaaatctc
     2941 attcacaaac agcaatttgc ctgtattttt taggcagaat agcattcctg aaaaaggaat
     3001 acaaaaatgc ccgggagtat tattatgaaa gtattaatat ttactgtgaa accggaaata
     3061 aaaaagacat tgctctcaat atattaagac tggcagaatt tcatggtttt aaaggacata
     3121 acattcaatc gacaaaactg cttgggttta taaaaaataa atattttgaa tcaaataaga
     3181 ttaaatttcc attggcagat cagatcattt ttgataaact aatattggaa ttgaaagaga
     3241 aattgaatga aaaggatttt ttgaaatatt atgaagaagg gaaattaatt acacatgatc
     3301 aggcgattga aattgcaatc agcaattagc aaaatcatta attcattgat cactgtttca
     3361 atatttattc agatacaagt atataaaaat tagatttgat actgtacccg gtttttaaaa
     3421 ttacttttta caggaaatca aaaaaattgt ggtcaacaat acagttaata atgatattaa
     3481 tttagttatt ttggaataaa ttttttaaaa ttagtaaatg agcagcatat taaaatctaa
     3541 agcaaagtca actattcctg ccaaaaaact gagtcaagaa aattcattta ataataatga
     3601 atggaaactc aaaacagaag aacttgaaaa aaatgttgcc gaaaagactg ctgatcttat
     3661 aatcaaaaac cgtgatcttg aaattgaagc ttctcttgaa cgcgtgcggg caattgctat
     3721 ggaaatgcgg gagcgtggag acctgcttga cgtaagtaaa attttgttta ttgaacttaa
     3781 aaatctggga ttcagtgaga taagaaatgc aatgatcaac attcatgatc atgaaaataa
     3841 aacttatctg aattatgatt attctgatac gcttggtaaa agcatttcac atatacacta
     3901 tgatacagat cctacgattg aaaagcagat aaaacagatc cgcaaaggga aagatgcatt
     3961 ttccgaaaca ttgttcaaag gtaaagaatt gacagaatgg aaaaggttaa ggaaaatcag
     4021 aggtgagaag gacgatccgc gtcttaagaa aatttcagca ctttattatt atttttattc
     4081 cataggaaac ggttcgatag gaatttctac atttgacggt ctcagcaaaa ataaattaga
     4141 aatattaaag cgtttcagaa atgtatttga atttgcatac aggagatttg aagatgtttc
     4201 aaaagcggaa gcgcaaacac gtgaagcaaa gatagaagcg tcacttgaac gcgtgaggac
     4261 aatagctatg ggaatgcgaa agcccaacga cctgctggat ataagtgaaa ttctttataa
     4321 agaacttaaa gatcttggat tcagcgagat cagaaatgcg atgataaata ttcataatga
     4381 tgaggaaaag acttttctga attatgatta ttccgacaca ctcggtaaaa gcataactcc
     4441 tttattttac aatattcacc cgttggttga aaaacaaata aagcaggtca ggaaaggaaa
     4501 ggacgcgttt tccgaaactt cgttcaaagg taaagaatta gcggaatgga aaagattcag
     4561 aaaaagcaga ggtgagaagg acgacccgcg tcttaataaa actacatcac tctattatta
     4621 tttttattca attggaacag gctcgatcgg gatttcaaca tttgacggtc tcagcaaaaa
     4681 taaattagaa gttttaaagc gtttcagaaa tgtatttgaa tttgcttaca gaagatttga
     4741 agatgtttcg aaagcggaag cgcaggcaag agaagcaagg attgaaattg cattggaaag
     4801 agtccgcagc aggacaatgg ctatgcaaag aagtgaggag ctttctgaaa ctacattcat
     4861 tcttttccgg caattccttg aacttggaga agcacccgac cagatcacaa ttggaattat
     4921 tgatgaaaaa gaaaaagtga tcgatttctg gactacgatg gaaggacaaa aaattagcag
     4981 tatggttaaa tttcctgtta atgatactga tcttatgaaa aagatctatc tggcatggaa
     5041 gagccagaaa aaatcaattt caattgagat caaaggaaag gaattacatg attacattga
     5101 atacaggatc aggctgacag gagtatcggg gaaaaatgat catgttcatg atcgtcgtta
     5161 cattcaggca gcgttttttt caaaaggttt gatcactatt tccaccaatg atccgcgttc
     5221 agatgaaacc atgcaactgc tcgaaagatt tgccggagtt tttgatctta catatacaag
     5281 atttctggat ttgaaacaag ccgaagcaca gacgaaagaa gctaagatcg aactttcttt
     5341 ggaaagagtc agggcaagaa ctatggcaat gcacagcagt gatgaacttc aggaagcggc
     5401 agttttattg tttcagcaga taagagaact cggtgtggat acaggcagct gcggttatgt
     5461 tatatgggaa aatgaaaaaa agaatgcgac tgtctggatg agttccccag agggaggcat
     5521 tcaggatcca tttaaattac ctcacacgaa aagtaaaatt tataaagaaa tatattcagc
     5581 aaaggaagaa gggaaagagt tttttataac agaagtgaaa ggcgcagaac tgaaaaaaca
     5641 ttttgattat ctgacaacag ttcctgggat tggtgaaaaa attatacagt taagaaaagc
     5701 ccgttataaa tttcctgaaa caattgcata taacattgca tttttcaaac agggttattt
     5761 atcatttcat acacatgagc agaaacccga tgcttatgat atattccgac gtttcgcaaa
     5821 tgtatttgaa cagacatata ccagattcct tgatctgcag aaagcagaag cgcaggcaag
     5881 ggaagcgcag gttcagcttg cacttgagag agttcgtgca agaacgctat caatgcatga
     5941 tagtgatgaa cttgaagcgg tagcagatat tttatttttg caattacaag ttcttggaat
     6001 ttttacttat cgatcttaca tcggaatttt tcggaattcc agattagctg atattttcgg
     6061 agctggtgaa ataaaagaat tcacgaaaac aattttcgat gaaaccaatg gattgataag
     6121 taaaattttt gtcccatggc gaaaaaaaga tcctacagca ataattaata caaatggaaa
     6181 tgaattcgaa tgtattgcat atttttcaag cgggtttgtt gcaataacat ctcctgatta
     6241 cccttcctca gaaacaatga atttgctgga gagatttgtc ggtgtattca atctaacata
     6301 cactcgtttt ctcgatttgc agcaatcaga agcacaggca cgagaagcaa agattgaagc
     6361 ttctctcgaa agagttcgca gcagaacaat gggtatgcaa aaaagtgacg agctcaaaga
     6421 agttattcaa gttgtgtatg atcagttggt tcagttaaat atctttgttg aacacagcgg
     6481 gtttattatg gattataaaa cgagggatga tatgcatatc tggcttgccg acaagtatat
     6541 ggttccattc gaagtaacaa ttccatactt tgattgtccg cactggaaca gttttgttga
     6601 agcaaaagaa aaaggaattg atttctttgc caaccatctt tcttttaaag aaaaaaataa
     6661 attttacaaa gctttattta agttgatccc cggcgtacct gaagaaacac tggaatatta
     6721 tcttacctgc ccgggtcttg caatatcaac ggtattgtta gataatgtag gtttgtatat
     6781 agagaatttt tcgggtactc cttattctga cgaagaaaat aaaacgctca tgcgctttgg
     6841 aaaagttttc cagcagactt acacacgctt tctcgatctg caaaaagcag aagcgcaggc
     6901 aagagaa
//
LOCUS       DAORVQ010000174         6890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6787_length_6890_cov_5.307388, whole genome shotgun sequence.
ACCESSION   DAORVQ010000174 DAORVQ010000000
VERSION     DAORVQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_6890_cov_5.307388"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..3033
                     /gene="uvrA"
                     /locus_tag="PLX80_11800"
     CDS             181..3033
                     /gene="uvrA"
                     /locus_tag="PLX80_11800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQY21519.1"
                     /translation="MSESHLDKIIVKGARVHNLRNVDIEIPRDSLVVFTGVSGSGKSS
                     LAFDTIYAEGQRRFMETLSAYARQFIGGIERPDVDKIEGLSPSISIEQKTISQNPRST
                     VGTVTEIYDFLRLLYARCGTQYSPESGNAVTRQNTDQILSSILDKKDGTKIIILAPVV
                     KGRKGHYRELFEDIASQGFTKVRIDGEVKDITPGMKTERFKIHDIEIVIDRVAVNDNS
                     KARIAESLEAALRQGDGVVIINDGNEDKFYNERLSDIETGRSFSETAPNSFSFNSPYG
                     WCNDCFGLGEKKEIDVDKIFPDRNLTINLGAVEPLGKPRGTWIYSIFKGMIENEGLDF
                     DTPIIKMPDDVIQKILFGSSEKVVYEHKNSQGRVSNYKHRFDGVLNYVKRYSDDTSSE
                     TIRQWAESFMTISKCKTCNGGRLKIDSLWVKIDNRNIHDITTMSIKKCRDFFEDLNLD
                     ERKMIIASQIIKEIISRLDFLLNVGLEYLTLDRSARTLSGGEAQRIRLATQIGSQLTG
                     VLYILDEPSIGLHQRDNIKLIESLKMLRDTGNSVIVVEHDKEMIEACDFVIDLGPGAG
                     EHGGEVVAFGHPQNLNGHSLTGDYISGKRKIEIPSVRREGSGKSIILKGASGNNLKDV
                     TLEIPLGKFICVTGVSGSGKSSLINETLYRILSKKFYKSPDTPLPFKTIEGLSMKKEK
                     WREKIIDKIIEIDQTPIGRTPRSNPATYTGLFTFIRDIYSNLPDSKIRGYKPGRFSFN
                     VKGGRCDACEGAGLQKIEMNFLPDVYVVCEVCEGKRYNSETLEVKYKEKSIADVLGMT
                     VEEAVGFFDAVPSLNRKLQTLFDVGLGYIRLGQQATTLSGGEAQRVKLSTELSKIQTG
                     RTLYILDEPTTGLHFEDINMLLKVLNKLTDKGNTVIVIEHNFDVIKTADWIIDLGPEG
                     GEAGGEIIATGTPEDIVKNFMETSYTAKYLKDELKK"
     gene            complement(3034..3663)
                     /locus_tag="PLX80_11805"
     CDS             complement(3034..3663)
                     /locus_tag="PLX80_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21520.1"
                     /translation="MKKLILVSIFILAVSSVEIAQSQTIKENFEAGKKAFYADSFDEA
                     NKLFNPILQMETENYETFFYKGLIYEIYFDFDRALSELTNAIEAKPNESETYFRRGIV
                     YDKKDMLKEAIKDYTKTIELNENNVDAYFSRATDYQELKEYGKAIDDYSDAIDLNPDD
                     DISFYNRGLLYKETGDFENAINDFNQAIQIDSIWQSELGREIENMKKGK"
     gene            complement(3692..6550)
                     /gene="uvrA"
                     /locus_tag="PLX80_11810"
     CDS             complement(3692..6550)
                     /gene="uvrA"
                     /locus_tag="PLX80_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQY21521.1"
                     /translation="MYNDPKRFIIIKGARVHNLKNIDINIPKYKLIVFTGVSGSGKSS
                     LVFDTVYAEGQRRYVESLSSYARQFLERMNKPDVDFMSGLAPSMAIEQKTQIKTSRST
                     VGTTTEIYDYLRLLFARVGKTYSPVSGKEVRKDSPESIIKEITASEKEFPKKIFVMSD
                     FVLENAEELKLLITDLKSKGFYRIIEGNEITDLNEIDEKKFVKNFFRNKNKAEGDEEK
                     WKLTVLFDRLMFDPNDTESVSRLNDSIELSYRESEGYVTIREFSGDHYSDLYFNKYME
                     LDGIKFEDPQPRLFSFNNPYGACSKCQGFSRTMDVDRDLVIGNKKLSVFGGVINVFNT
                     PKHSKHLTDLITEADSFGLDIHLPYNKLDSKQKDFIFEGGKKYIGINRFFKMVEREAA
                     YKLHYRVLLNRYRAFTTCSECRGARLRKEALYIKVAGKNIFEIVKLKIEEAYEFFQNL
                     KLSEYDKKISERITEEIVSRLKYLNDVGLTYLSLDRLSNTLSGGESQRINLSTSLGSS
                     LVGSIYVLDEPSIGLHPRDNQKLIDIMKSLRDIGNSVLVVEHDSDMMKEADEIVDMGP
                     FAGEKGGEKIFQGTYEEIIKSKDSLTGKYLSGKLKINLPKLRRLITDETFFITIKGAR
                     ENNLKDVNVKIPLNMFVCITGVSGSGKSTLVNNILYGDLKRKLEGTYNERIGEHDSLT
                     GDKYINAVEMIDQNPIGKTSRSNPVTYIKAFDLIREAFSNTSESKRKNFYAGHFSFNV
                     SGGRCEKCEGTGVINIEMQFMADIVLECEECKGKRYRDEILELKLKGNEGIRKNISEV
                     LDMTVSEAVNFFKPFPKVVKKLKVLEDVGLGYIRVGQSATTLSGGESQRVKLAYHLTF
                     QEKGKKTLFIFDEPTTGLHFYDVSKLLKCFDELIKKGNSVVVIEHNLEVIKCADHVID
                     LGPESGDEGGYIIAEGTPEEISKVKKSYTGRFLKNVLK"
BASE COUNT         2179 a         1276 c         1154 g         2281 t
ORIGIN      
        1 tgttactttt gagcctgccc tcgagtgttc ttatcggggg tcgttcaaaa aaagttaata
       61 gaaaatagtg aatcttgaaa gttgtattga attgttttgg attgatatga aattttctca
      121 attcagcatt aaattttaga ttgtaaaaac aacctgactc atttattttc catgatattt
      181 atgtcagaat ctcatttaga taaaataatc gtaaaaggtg cgcgagttca taatctcaga
      241 aatgtagata ttgagatccc gcgtgacagc cttgttgtgt tcactggcgt ttctggttca
      301 ggaaaatctt ccctggcatt tgatactatt tatgctgaag gtcagagaag atttatggaa
      361 actctttctg cttatgcccg gcaatttata ggcggtatag aacgccctga tgtagataaa
      421 atagaaggac tttcaccatc catttccatt gagcaaaaga ccatttcgca aaatccgaga
      481 tctactgtag gcacagttac agagatctat gatttccttc gtctgttata tgcacgatgt
      541 gggacgcagt attctcccga gtcagggaat gcagtaacca gacaaaatac agatcagatt
      601 ctcagcagca tacttgataa aaaagacgga acaaagatca ttattcttgc ccctgttgta
      661 aagggaagga aaggtcatta cagagaattg tttgaggaca ttgcttcaca gggatttacg
      721 aaagttcgga tcgacggtga agtaaaagac attacccctg gaatgaaaac agagcgtttt
      781 aaaattcatg atattgagat agtgatagac agagttgcag ttaatgataa ttcgaaagcg
      841 agaatagcag aatccttaga agctgcactc agacaaggtg atggggtggt aattatcaat
      901 gacggaaatg aagataaatt ttataatgaa agattatcag acattgagac cggcagaagt
      961 ttttcagaaa ctgctccgaa ttcattttca ttcaattcac cttacggatg gtgcaatgac
     1021 tgttttggac ttggagaaaa aaaagaaata gacgttgata aaattttccc tgacagaaat
     1081 cttacaatta atcttggtgc tgttgaaccg cttggaaagc ctcgtggtac ctggatctat
     1141 tcaatattca aaggcatgat cgagaatgaa ggattggatt tcgatacacc tataataaaa
     1201 atgcctgatg atgtcattca aaaaattctt ttcgggtctt cggaaaaagt tgtttacgaa
     1261 cataagaatt cacaaggcag agtaagtaat tacaaacaca gatttgacgg agttttgaat
     1321 tacgttaaaa gatattctga tgatacttct tcagaaacta tcagacagtg ggcggaaagt
     1381 tttatgacaa tttcaaaatg caaaacctgt aacggtggac gtctgaagat tgattcactc
     1441 tgggtaaaaa ttgacaacag gaatattcac gatatcacaa caatgtccat taaaaaatgt
     1501 cgtgactttt ttgaagatct taatcttgac gaaagaaaaa tgatcattgc atctcagatc
     1561 attaaggaaa ttatctcacg tctcgatttt cttctgaatg taggacttga atatctgact
     1621 cttgacagaa gtgcaagaac tctgtcgggt ggtgaagctc agcgaatcag acttgcaact
     1681 cagatcggat cacagcttac cggtgttctt tatattcttg atgagccttc aatcggactt
     1741 catcagagag ataatatcaa actcattgaa tcattaaaga tgctgagaga tactggcaac
     1801 tctgtaatag tagtagagca tgataaagaa atgatcgaag cttgcgattt tgtaattgat
     1861 ctcggacccg gtgcaggtga gcatggcggt gaggttgtcg cattcggtca tcctcaaaat
     1921 ttaaacggac attcacttac tggtgattat atctcaggta aaagaaagat agaaattcct
     1981 tcagtcagaa gagaaggttc aggaaaatca attattctaa aaggagcttc aggtaataat
     2041 ctgaaggatg taactttgga gatcccgctc ggaaaattta tatgtgttac aggtgtcagc
     2101 ggctcaggaa agtcttcatt aattaatgaa actctctacc gcattctttc aaaaaaattc
     2161 tataaatctc ctgatactcc actccctttt aaaacgattg aaggactttc catgaaaaaa
     2221 gaaaagtgga gagaaaaaat aattgataag ataattgaaa tagatcagac tcctatcggc
     2281 aggactccaa gaagcaatcc cgctacatat acaggtttat tcacttttat aagagacata
     2341 tattccaatc ttcccgattc aaagatccgc ggatataagc ccggcagatt ttcatttaat
     2401 gtgaaaggcg gaagatgtga tgcatgcgaa ggtgcaggtc tgcagaagat tgaaatgaat
     2461 ttccttccgg atgtttacgt cgtttgtgaa gtttgcgaag gaaaaagata taacagtgag
     2521 actctggaag taaagtataa agaaaaatca attgcagatg tccttggcat gacagtagaa
     2581 gaagctgttg gattttttga tgctgtacca tctctcaaca gaaagctcca aactttattt
     2641 gatgttggac tgggttatat aagacttggt cagcaggcga ctacactctc gggaggagaa
     2701 gctcaaagag taaaactttc gactgaactt tctaaaattc aaacgggaag gacactttac
     2761 atacttgacg aaccgactac aggtcttcac tttgaagaca ttaatatgct tctgaaagtt
     2821 ttaaacaagc taactgataa aggtaatacc gtaattgtga tcgaacataa ttttgatgtt
     2881 ataaaaactg ctgactggat tattgatcta ggtccagaag gaggtgaagc tggcggtgag
     2941 ataattgcaa caggtactcc cgaagacatt gtaaaaaatt ttatggaaac ttcttacaca
     3001 gcgaaatatc tcaaagacga attaaaaaaa taattacttt ccttttttca tattttcaat
     3061 ttcccttcca agctcgctct gccagatgct gtctatttgt atcgcctgat taaaatcgtt
     3121 aattgcattt tcaaaatctc cggtctcttt ataaagaagt cccctgttat aaaaagatat
     3181 atcgtcatcc ggatttaggt ctatcgcatc tgaataatcg tcaattgctt ttccatattc
     3241 tttaagttcc tgataatccg ttgccctgct aaaataagca tcgacattat tttcatttag
     3301 ttcaattgtt ttagtataat ctttgattgc ttctttaagc atgtcttttt tatcatagac
     3361 tattcccctt ctgaaataag tctcactttc atttggttta gcttctattg catttgtaag
     3421 ttctgataaa gctctgtcaa aatcaaaata aatttcatag atcaaccctt tataaaaaaa
     3481 ggtttcatag ttttctgttt ccatttgaag aattggatta aataatttat tagcctcgtc
     3541 aaaactgtca gcataaaaag cctttttacc ggcttcaaaa ttttccttta tagtctgtga
     3601 ttgtgctatt tctacagatg atacagctaa aataaaaatt gaaactaaaa ttaatttctt
     3661 cattacaaaa ctgattattt atattaataa attattttaa aacatttttt aaaaatcttc
     3721 cggtataaga tttttttacc ttagagattt cttcaggtgt tccttcagca attatatatc
     3781 ctccttcatc accgctttcg ggtccaagat cgattacatg atcagcacat ttgattactt
     3841 caagattatg ctcgatgaca actacagaat tacctttttt aatcaactca tcaaaacatt
     3901 taagcagttt ggaaacatca taaaaatgaa gtcctgtagt aggttcatca aatatgaata
     3961 atgtcttttt tcctttttcc tgaaatgtca gatgatatgc aagtttcact ctctgcgatt
     4021 caccgccgga taaagttgtt gctgactgac caactctgat ataaccaagt ccgacatctt
     4081 ctaatacctt aagttttttt acaacttttg gaaatggctt aaaaaaattc actgcttcac
     4141 taacagtcat atcaagaact tcgctaatat tttttctgat accttcattg ccttttaatt
     4201 tcaattcaag aatttcatct ctgtatcttt tacctttgca ctcttcacat tcaagtacta
     4261 tgtcagccat aaactgcatc tcaatattga ttacacctgt accttcacat ttttcacatc
     4321 ttccgccaga tacattaaat gaaaaatgtc ctgcataaaa gttttttcgt tttgattcag
     4381 aagtatttga aaacgcttcc ctgatcagat caaaagcttt aatatatgtg acagggttac
     4441 tgcgtgaagt tttgccaatc ggattctgat cgatcatttc gactgcattg atatatttat
     4501 caccggttaa agaatcatgc tcgccgatcc tttcattata agtaccttcg agttttcttt
     4561 tcagatctcc gtatagaata ttatttacca aagtagattt tcctgaaccg ctgacacctg
     4621 ttatacaaac aaacatattt aaaggaatct taacatttac atccttaaga ttattttctc
     4681 tggcaccttt aattgtaata aaaaatgttt catccgttat tagtcttctt aactttggca
     4741 aatttatttt caactttcca ctaagatatt tcccagtgag tgaatctttg cttttaatta
     4801 tttcctcata agttccctga aaaattttct cgccgccctt ctcccctgcg aaaggtccca
     4861 tgtcaactat ctcatcagct tccttcatca tatcactgtc atgctctact actaatactg
     4921 agtttccaat atcccgcaga gatttcataa tgtcaataag tttttgattg tcacgaggat
     4981 gaagtccaat tgatggctca tctaaaacat agattgaacc aaccagtgat gatccaagtg
     5041 aagtggatag attgatcctc tgtgattcgc ctcctgacag agtattagat agtcggtcaa
     5101 gagaaagata agtaagacca acatcattta gatatttcaa acgggaaact atttcttccg
     5161 ttattctttc tgaaattttc ttatcatatt cacttaactt tagattctga aagaattcat
     5221 aagcctcttc gatcttcaac tttactattt caaaaatatt ttttccggcg accttaatat
     5281 aaagtgcttc ctttctcagt ctggctcccc ggcattcgct gcatgtagtg aatgctctgt
     5341 acctgttcag cagaactctg taatgaagtt tataagcagc ttctctttct accattttaa
     5401 aaaatctgtt tatgccgata tactttttac cgccttcaaa aataaaatcc ttttgtttac
     5461 tgtccagttt attatatggc aaatgtatat ccagaccaaa gctatccgct tcagtgatca
     5521 gatctgtaag gtgctttgaa tgtttaggtg tattaaaaac atttatcact cccccaaaaa
     5581 ccgaaagttt cttgtttcct atcacaagat ccctgtctac atccatggtc ctgctgaaac
     5641 cctgacattt actgcatgcc ccgtatggat tattaaagga aaaaagtctt ggctgaggat
     5701 cttcaaattt aattccgtca agctccatat atttattaaa atacagatcg gaataatgat
     5761 caccgctaaa ttccctgatc gtcacatacc cttcactttc tctgtaggaa agttctatgg
     5821 aatcatttaa tcgcgaaaca gattccgtgt catttggatc aaacatcagt ctgtcaaaca
     5881 gcacagttaa cttccatttt tcctcatcac cttctgcttt atttttattc cgaaaaaaat
     5941 tcttaacaaa tttcttttca tcaatctcat tcaaatctgt aatttcatta ccttctataa
     6001 ttctgtaaaa tccttttgat ttaagatcag tgataagaag tttcaattct tcagcgtttt
     6061 ccaatacaaa atcagacatg acaaagatct tttttggaaa ttctttttct gaagctgtga
     6121 tctcttttat aatactttcg ggagaatctt ttctgacttc tttaccgctg actggagaat
     6181 atgttttccc tacccttgca aatagcagac gcagataatc ataaatttct gtagtagtgc
     6241 cgactgtaga acgggaagtc ttgatctgag tcttttgttc gattgccata gaaggtgcaa
     6301 gtcctgacat aaaatcaaca tcaggtttgt tcatcctctc aagaaattgt ctggcgtaag
     6361 atgaaagact ttcgacatat cttctctgtc cctcagcata aacagtgtca aagacaagcg
     6421 aagattttcc cgaaccactt actcctgtaa agacaatcag tttatattta gggatattga
     6481 tatcaatatt cttaagatta tgaacccgcg caccttttat aataatgaat cttttaggat
     6541 cattgtatat gtctgaacct gagtcagagc ttatgaatgc cgacttttcc ttagtctttt
     6601 tagtaatgcc tttaccatta gaaataacag tatcagcagt atctgaagac tttacagact
     6661 tatttttaga tttggttttt gccaatgagc tttatataaa acgtttaaat taattcattc
     6721 accaatcaat ttcagtccaa atttcactac tgattctgga aaatctttaa cactgtagca
     6781 gagaattaat aaatagataa ttcgaatgag ctcgaaacga aatttcaatt ttcattaaaa
     6841 ccccttttta tctgaactta tttatgaatt actttatttc acaatcttaa
//
LOCUS       DAORVQ010000175         6877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6812_length_6877_cov_5.699795, whole genome shotgun sequence.
ACCESSION   DAORVQ010000175 DAORVQ010000000
VERSION     DAORVQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6812_length_6877_cov_5.699795"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLX80_11815"
     CDS             complement(<1..567)
                     /locus_tag="PLX80_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY21522.1"
                     /translation="MLNKRGNSSFQERKDIVSKGISILGKDRIECLAADREFGSGKFF
                     RFLKKAEINFHIRIKKTTVMTKYKSKVSSVTTMLSHLKLHKYIIVPGKKIIYNEEVYV
                     SEKKTVGNDYLIIASNSNPDEAQAMYKQRWTIENLFGNMKTKGFFLEKTHLKEDEKLK
                     KLIALLTIAIRWCYLIGLWIEKSITVRVK"
     gene            1083..2471
                     /gene="rho"
                     /locus_tag="PLX80_11820"
     CDS             1083..2471
                     /gene="rho"
                     /locus_tag="PLX80_11820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQY21523.1"
                     /translation="METKTGNKSAHNSNSNSKDSKELKDSRDSKDSKDPKESNDSNAS
                     KDAKNVTDFKSKKVSELIDIAKDLGVSGYSDLKKQELIFKIIEAQAKRDGFAYAVGVL
                     EVLPDGYGFLRSVDYNYLPSPDDIYVSPSQIKKFLLRTGDTVSGQVRPPKEGERFFAL
                     LKVESVNFESPDTIRDRILFDNLTPLYPEERFNLESTPGEYSMRIMDLFTPIGKGQRG
                     LIVSSPKSGKTILLQTLANSITRNHPEVHLIVLLIDERPEEVTDMERNVNAEVISSTF
                     DEPPERHVQVSEIVLQKAKRLIEAKKDVVILLDSITRLARAHNTVIPHSGKILSGGVD
                     ANALHKPKRFFGAARNIDEGGSLTIIATALIETGSRMDEVIFEEFKGTGNMEIVLDRR
                     ISDRRVFPAIDLNKSGTRKEELLLDSDVLNRVWLLRKIVADYNPVEAMEFLLGKMKGT
                     KSNKEFLASMNS"
     gene            2498..4060
                     /locus_tag="PLX80_11825"
     CDS             2498..4060
                     /locus_tag="PLX80_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQY21524.1"
                     /translation="MKKVILINSVSEDIRIAITEDGKLAEFFLDTPAKERNVGDIYLG
                     KVGKVIPGIRAAFIDLGFKQDAFLHFSDIGNTLSEYASMIGDDPDVEEDDEDEEEETP
                     PVNTREDYRKNPILEKGQDIVIQITKEPVASKGFRVTTKVSLPGRYLVLIPFEKKIGL
                     SKQIYNPREKRRLRSIVKSNVPKGFGVIIRTVAAGQEENLILDDLNSLIVMWNDIQAK
                     AKSSQTPFILYKDVSTTSSVVRDMFKEDITKVIVDSKKLYREIKTYVDETSPEFSGKI
                     EQYTGNQPLFDVYNIEKQIEESLQRKVWLKSGGYIIIEATEAMTVVDVNSGKYARHRD
                     QEVNTLNTNLESAKEIVRQIRLRDIGGIIVIDFIDQYDEKNRKKLYEEVKKEFRNDRA
                     KSTILPVSEFGIVEITRQRIRQNIIHTISDRCPMCRGTGHVQSKATFINRFERWMKRY
                     QGGNNGKSIIVTVNPFIKNYLTEGFISRVTKLKISNFLNIQLVEDENLALDEFKVFSK
                     KANKDVTEEFDN"
     gene            4858..6129
                     /locus_tag="PLX80_11830"
     CDS             4858..6129
                     /locus_tag="PLX80_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY21525.1"
                     /translation="MENWKKNLYSIWIAQFVAMAGMSMVIPFLPFYIRELGVTEIHEL
                     EFWSGLVFSGPFILSFILTPVWGMLGDRFGKKTMVMRAIFGLALSQLLIGFSADVYQL
                     FIFRMVQGGISGFIASSLALVSSSTPKEKSGYAIGILQTSISSGTVIGPFIGGIISDM
                     TSYKTVFFVTSALCIVSGLLVLINVKEPPKQISSRRFTLSDNYKYVFSRSFLLISMLS
                     ILFLQISITVAQPVFALFVESITTGTEYLSTLTGAIFATLGVFSVISSPWWGKRNDTK
                     SFKKNIIIAITGAGIAYCLHSFVYDPYMLFPVRAFLGLCVGGIIPVIYANINKYISDE
                     RKSGIMGIASSFTLLGNLLGPLICTALLFKIDMRYIFLIAGIMLFVNAFIIYIKVKEF
                     PKSKEFLLNGTGVNFKEIHNSDIDQNLPKNN"
     gene            6260..6574
                     /locus_tag="PLX80_11835"
     CDS             6260..6574
                     /locus_tag="PLX80_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HQY21526.1"
                     /translation="MKPKKLKRSEDNKLSIEWNDNSVSELDIKMLRDECPCVHCKGET
                     VLFSSYIPIKDPFKAAGFYELEKIEPIGNYAIQIFWKDGHNTGIYSWEYLKELSEREN
                     KN"
BASE COUNT         2370 a         1145 c         1224 g         2138 t
ORIGIN      
        1 cttaactcta acagtaattg atttttctat ccagagccct atcagataac accatcgtat
       61 tgctatagtt aataatgcaa ttaatttttt taatttttca tcttctttca aatgtgtttt
      121 ctctaaaaaa aatccttttg ttttcatatt accaaagaga ttttctattg tccatctctg
      181 cttgtacata gcctgagctt cgtccggatt actgttactg gctattatta aatagtcatt
      241 cccaacagtc ttcttttcgc ttacatatac ttcttcattg tatatgattt tttttcccgg
      301 cactattatg tatttatgta atttcaagtg tgacaacatc gttgttactg aggatacttt
      361 tgatttatac ttcgtcataa cagttgtctt ttttattctt atatgaaaat taatctctgc
      421 ttttttcaaa aatctgaaaa attttccgct tccaaattca cgatctgctg ctaaacattc
      481 tattctgtct tttcccaata ttgatatgcc ctttgaaaca atatctttcc tttcctgaaa
      541 tgatgagttt ccacgtttat tgagcaattt tggatcagat ttttattttt tattcaaaag
      601 ttttgtcgtg tacagaggtt tgtgaatcat tatttccata gaaaacgaaa tggcgtccca
      661 aatatttttt cagtgaatga aaaatgctgg tacatatatt ttaaaataat aatgattaca
      721 atataaatta tttattacta agactccctg atcttaaatt gtgaattctc acaaattgaa
      781 agaaagccat tttttaattt tctgccggct cgaaaaattt cacatcttat gatgaaagat
      841 cctttgagac tatgatattt tgggaataaa ttatttaatc cgaattttta aaggttttaa
      901 atttataact tgataatatt tattatttgt aatagttttg ggcacatcaa ataacacacc
      961 ttatttctgc ttaaggaaat tattgaaaac attacagttc gttatcatat taaaattttt
     1021 ttcgttaata atactttata attaatcacc ccgattttcc ttcacaagaa aggacacaac
     1081 ttatggaaac aaagaccggc aataaatcag cacacaattc taattcaaat tctaaagact
     1141 caaaagaatt aaaagactca agagattcaa aggattcaaa agatcctaaa gaatcaaatg
     1201 attcaaatgc ttcaaaagac gcaaaaaatg tgactgattt caaatcaaaa aaagtatctg
     1261 agctcattga tatcgcaaaa gatctcggag tatcaggata cagcgacctg aaaaaacagg
     1321 aattaatatt taagatcata gaagctcagg caaagagaga cggatttgct tatgcagtag
     1381 gagtactgga agtattacct gacggatacg gattccttcg ttcagtggat tataattatc
     1441 ttccttcacc tgacgatatt tatgtatcac cttcacagat taaaaagttt ctgctgcgaa
     1501 caggtgacac agtcagcggg caggtaagac ctccaaaaga aggagaaagg ttttttgcat
     1561 tactaaaagt cgaatcagta aactttgaaa gtcccgatac gatacgcgac aggatcctgt
     1621 ttgataatct gacgcccctt tatcctgaag aaagatttaa tcttgaatcc acacccggtg
     1681 aatattccat gaggatcatg gatctgttta caccgatcgg aaaaggacaa agaggtctga
     1741 tagtgtcttc tccaaaaagc ggtaagacaa ttttactgca gacacttgca aacagcatta
     1801 caagaaatca tcccgaagta catttaatag ttttactaat agacgaaaga cctgaagaag
     1861 taaccgacat ggaaaggaat gtaaatgccg aggtaattag ttcgacattc gacgaaccgc
     1921 cagagaggca cgttcaggtt tctgaaattg tacttcagaa agcaaagaga ctgatcgaag
     1981 caaagaaaga cgtagtcatt cttctcgata gtattacaag acttgcaaga gcacacaaca
     2041 cagtcatccc acacagcgga aaaatccttt ccggtggtgt ggatgcaaat gcacttcaca
     2101 agccgaagag atttttcgga gctgcaagaa atatagatga aggcggaagt ctgacaataa
     2161 ttgctactgc gcttatagaa acaggaagca gaatggatga ggttattttt gaagagttca
     2221 aaggaacagg taatatggag atcgtacttg acagaagaat ttccgacaga agagtattcc
     2281 ctgcaataga cttaaataaa tccggtacca gaaaggaaga gctgcttctt gacagcgatg
     2341 tactcaacag agtatggctg cttagaaaga tcgtagctga ttataacccc gtagaagcta
     2401 tggaattttt gcttggcaag atgaaaggca cgaagtccaa taaagagttc ttagcttcca
     2461 tgaattcata acgcatctgt gaaaatttgc tgactttatg aaaaaagtta tactaatcaa
     2521 ttcggtttcg gaagacatca gaatagcaat taccgaagac ggtaagctcg ctgagttttt
     2581 tttagacacc cccgcaaaag aaagaaatgt cggtgacatt tacttaggaa aagtaggtaa
     2641 agtaatcccc ggtatcaggg cagcttttat tgaccttggt tttaaacagg atgcctttct
     2701 tcatttttct gatattggaa atacactttc tgaatatgct tcaatgatcg gtgatgatcc
     2761 tgatgtagaa gaggatgatg aagatgaaga agaagaaact ccgcctgtaa atacccgcga
     2821 agattacaga aaaaatccga tacttgaaaa aggtcaggac attgtgatac agatcacaaa
     2881 agagccggta gcaagtaaag gattcagagt aacgacaaaa gtttcactgc ccggcagata
     2941 tcttgtactt ataccattcg aaaaaaagat aggactttcc aaacaaattt ataatccgcg
     3001 tgaaaaaaga cggctaagaa gtattgtaaa atccaatgtc ccgaaaggat ttggagttat
     3061 cattaggact gttgctgccg gacaggaaga aaatttaata cttgatgacc taaacagtct
     3121 gatagtaatg tggaatgaca ttcaggcaaa agccaaatct tcacaaacac catttatatt
     3181 atataaggat gtttcgacta cttcatctgt ggtcagagat atgttcaaag aagatataac
     3241 caaagttata gttgattcca aaaaacttta cagggagata aaaacctatg tcgatgagac
     3301 ctctccggag ttttcaggaa agatcgagca atacaccggt aatcagccat tgtttgatgt
     3361 ttataatatt gaaaaacaga tcgaagaatc acttcaaaga aaagtatggc tcaagagcgg
     3421 cggatatatt attatagagg cgactgaagc aatgacagtc gtagatgtaa acagcggaaa
     3481 atatgccaga cacagggatc aggaagtcaa tactttaaat acaaatctcg aatctgcaaa
     3541 agaaatagta agacagatca gactgcggga tatcggaggt atcattgtaa tagattttat
     3601 agatcaatat gatgagaaga acagaaaaaa actttatgaa gaagtaaaaa aggaattcag
     3661 aaatgacaga gcaaaatcca ctatactgcc tgtcagtgaa tttggaatag tcgagatcac
     3721 gagacagcgg atcagacaga atataattca tacaatctcc gacagatgtc cgatgtgcag
     3781 aggtacagga catgtacagt caaaagctac ttttataaac aggtttgaga gatggatgaa
     3841 aagatatcag ggcgggaata acggcaagag tatcatagta accgtaaatc cttttataaa
     3901 aaattatctt actgaaggat ttatcagccg cgttacaaaa ttaaaaatca gcaacttcct
     3961 gaacatacag cttgtagagg atgaaaatct tgcacttgat gagttcaagg tattttccaa
     4021 aaaagctaat aaagatgtaa ctgaagagtt tgataactga tacatccctc caaccaaacc
     4081 ggtttaaaat aaaattatat atttacagtt tctacatttc ttttttgagc gatcaaaaat
     4141 gaaccaaaaa aatcgccgat ggtgattaat tgccggaaat tctgagcaat gtctacaaaa
     4201 atataaactc ctcatccgtt agaaaaacac ttacgtattc gtcaaacagt atatttttgc
     4261 ttaacaccat tgctcagaat ttcttaccgt ctatttatca caggcggatt atctcatata
     4321 agtttgctgt gctgtcctaa ataatttaaa aaaaggaata tggttttgaa atacttctat
     4381 ttaaaacaaa ttatcatttt tgttcaaatc aaccgctcac aaaagcaaca aaaataatca
     4441 tagaagtttt ttgttttcaa aaatcccgcc tgtaatatat tgccgataag aaatttgcga
     4501 agcaaatttt gagttggata gtttatttca atttcgaaaa gaatttaggc agtttattat
     4561 cccgggtact cgggacgact ttttttgtta cttttgagcc tgccctcgag tgttcttatc
     4621 gagggtcgtt aaaaaaaagt aagtagaaaa tattgaatct tgaattttgc aacgaaacgt
     4681 tttggacgga tatgaatatt tatgtactta aaaattttgt aaggatttag tcatttcaat
     4741 ttaatacaat tacatattct gactctatcc ttattctcaa ttgtaaaatt acattatcat
     4801 ttgtaacttc atatataatt ttataatcca tttttaatta accttacatc tttctgattg
     4861 gagaactgga agaaaaatct atatagcatc tggatcgcac agtttgttgc aatggcaggt
     4921 atgagtatgg taattccatt tctgccgttt tatattcgtg agcttggagt aactgaaatt
     4981 catgaactcg aattctggag cggacttgta ttcagcggac cgtttatttt atcatttatt
     5041 ctcactcctg tctggggaat gctcggcgat agattcggaa aaaagactat ggtaatgcgt
     5101 gcgatatttg ggttagcttt atcgcagctt ctgatcggat tttcagcaga tgtgtatcag
     5161 ctttttattt tcagaatggt gcagggagga atcagcggat ttattgcttc ctcgctggca
     5221 ttagtttctt caagtacccc caaagaaaaa tcaggatatg ctattggaat tctgcagact
     5281 tctatctcat caggaaccgt tattggtcct ttcatcggtg gaattatttc agatatgact
     5341 tcatataaaa ctgtattttt tgtaacatcc gcgctttgca ttgtcagcgg actgcttgtt
     5401 ctaataaatg ttaaagaacc tcccaagcaa atttcatcaa gacgttttac actttctgat
     5461 aattacaaat atgttttctc aagatctttt cttctgatat caatgctttc aatacttttt
     5521 ttgcaaattt cgataactgt ggcacaaccg gtctttgcac tatttgtaga atctataact
     5581 acaggaactg aatatctctc gacacttaca ggagcaatat ttgcaactct cggagtattc
     5641 agcgtgatct catctccatg gtggggtaaa agaaatgata cgaaaagttt taaaaagaat
     5701 attattattg caattacagg tgcaggtatt gcttattgtt tacactcatt tgtttatgat
     5761 ccgtatatgc tttttccagt aagagctttt ctcggcttgt gtgtcggcgg gataattcct
     5821 gtaatttatg caaacataaa taaatatatc agcgatgaaa gaaagagcgg tataatggga
     5881 atagcttcca gttttacttt gctggggaat ctgctcggac ccctgatttg cacagcacta
     5941 ctttttaaaa ttgatatgcg gtatattttt cttattgcag gtataatgct ttttgtgaat
     6001 gcatttatta tctatattaa ggtaaaggaa tttccaaaaa gtaaagaatt tttacttaac
     6061 ggtacgggag taaattttaa ggaaattcat aattcagata ttgatcagaa tttaccgaaa
     6121 aataactgat caaataaatc agattattca aattaatttt attgaagcca aaaaaattaa
     6181 aaagattatt tgtttccgca gagtcacatg ctcttcggac tctgcgtgta cagaattaaa
     6241 aaaaataaat taattatata tgaagccaaa aaaattaaaa agatccgagg ataataaact
     6301 aagtatagaa tggaatgata attcagtttc cgaactggac ataaaaatgc tgagagatga
     6361 atgtccctgc gtgcattgca aaggtgagac cgtactgttc agcagttata tacctatcaa
     6421 agacccgttt aaagctgccg gattttatga actggaaaaa atagaaccta tcggaaatta
     6481 tgcaattcag atattctgga aggacggtca caataccggt atttattcgt gggagtattt
     6541 aaaggaatta tctgaaaggg aaaataaaaa ttaattcatc acatttgtca gatagtgtca
     6601 aaaataattt gatgtatata agaaaatgaa gtttattaaa tataaaaacc cctccaataa
     6661 attaattttg aatacaaact tatcatatcc gtccaaaacg tttcgctgca aaattcaaga
     6721 ttcattattt cctgcttgct tttttttaac gacactcgat aagaacactc gagggcaggc
     6781 tcaaaagtaa caaaaaaagt cgcccgctgg taataaattg cataaattct tttcgaaatt
     6841 gaaataaaat atccaactca aaatttgctt cgcaaat
//
LOCUS       DAORVQ010000176         6821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_6922_length_6821_cov_6.223175, whole genome shotgun sequence.
ACCESSION   DAORVQ010000176 DAORVQ010000000
VERSION     DAORVQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6922_length_6821_cov_6.223175"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>2253)
                     /locus_tag="PLX80_11840"
     CDS             complement(172..>2253)
                     /locus_tag="PLX80_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21527.1"
                     /translation="GNVVINSGATFYSGNSSVYTVSIYGNLTNNGTMTGAGSSVRFFG
                     AVLVNTGSIASPYFTFDSTCTVSGNGTYTGLEIHISSNGNVTLLNDVTFSPAGTGSLF
                     EIKTGGIFTANGFIFTFSSGRFAILSGGTVVNSGTVRTQNTVQIDPRAGSNFNANLNV
                     FSGTASVAATTNPYTCRVNGNVVINAGATFYSGNSVVYVVSIYGNLTNNGTMTGAGST
                     VVFFGAVFINNGSVATPEFKFDSTSTLSGTGSWSTTANIISGSVVTLTSGHQFHSTNI
                     NTGAVFLMGSNTAKFTASNPIIQNGTLSNTGSTIEYNGSDVQNISAANITYYGLRINN
                     PTGTVLLANVPVNDTLSVIIGDLDLNGNVITVSPTGYLTETPGNTVKGNSGYIITTRN
                     LNAPSSLNVAGFGSILTTASNLGSTEIKRGHTPQNGLSGNTSILRYFDITPSTNTGLN
                     ATLVFKYDESELNGKVEPALTLFRSTNTGSSWVARGGTVNTVANNITLSGIDAYSRWS
                     ASSASAVVSTIKLIMEGFYNVPTTRLNMRDTARVYLRNSSSPYAIVDSAKSIIDSVTY
                     TGSFLFSNAGTGTYYLQTKHRNSIETWSKTPQSYTLGTGLNYDFTDNITKAFGNNMVQ
                     KSSKFCLYSGDVNQDGIIDIDDNQFIDNDAFNFGSGYVVSDVTGDDLVDLADASIADN
                     NALNFVGKITP"
     assembly_gap    2254..2353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2354..3953)
                     /locus_tag="PLX80_11845"
     CDS             complement(<2354..3953)
                     /locus_tag="PLX80_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21528.1"
                     /translation="MKKFLKCFAGLFLFMVLISSSADAQLYRSIASGNWNANSTWEVS
                     TNGGATWMSASSTPTSANSSQTTINHTVTVTANVSVSELNLMGTISINSGIVLTYPIT
                     TLTLNSGTSVTGAGTFQFSIGGNLYPLNGSNFSAILRVNSGILDVRNYQSYTATLNGN
                     VIIDAGAGLWTGESVIYTLIIKGSLTVNGTITGWGHLKFYGSSLSNSGNIEPYIFTFD
                     STTSASGIGTLKSSQIYINPNGNVTLTSNGVFAPNGGESLFEIKTGGIFSPNSNIFTF
                     SSGRFAILTGGTVVNSGTVRTQNTVTVDPRTGSNFNADLNVFSGTTTVTSNGNPNTCR
                     VNGNVVINSGATFYSGNSSVYTVSIYGNLTNNGTMTGAGSSVRFFGAVLVNTGSIASP
                     YFTFDSTCTVSGNGTYTGLEIHISSNGNVTLLNDVTFSPAGTGSLFEIKTGGIFTANG
                     FIFTFSSGRFAILSGGTVVNSGTVRTQNTVQIDPRAGSNFNANLNVFSGTASVAATTN
                     PYTCRVNGNVVINSGATFYSGNSSV"
     gene            4401..5834
                     /locus_tag="PLX80_11850"
     CDS             4401..5834
                     /locus_tag="PLX80_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21529.1"
                     /translation="MQNTKIIKFLKTLNQYEITQFKDFVNSPAFNKNKNVIKLFDELK
                     KFYPKFKNDDLSEEIIYGKVFPGEKFDYFKLRNATSDLLVLGKEFFSYIKFKKSNIKD
                     LFLLEELRKRNLDNIYEQTYKAAFKQNEEIKVKDEIYMYHKMNLTEELLSFNVIKKPN
                     AIFHFQQDMLDIFIRYSLIRFLKFYDVMLHEGGQNNYKYEMGMFEEVLKYLKNNKIDD
                     NPTLLVYYNIILLMKEKKDKNYYELKSLKTKYINELSARDRYMTWIHMNSYCVSMFNN
                     CSRTDLLREQFELMKENTEDDTRKLGKILYPDFLNEVKIAVRVNEFKWAEDYIVRFSE
                     KLEVEKESTLNFCYGYISYKKGDLDEALKLFSKTYFTLFIIKVQVKILQLQICFEKEY
                     FDQAVSLIDSFRHYLKREVTIKEIFRMSFYEFLRLTNELIRLRTNYYGSDNDFYKEKL
                     KNEISNLKNNQFGIKLWLKEKLNVESR"
     gene            complement(6123..6527)
                     /locus_tag="PLX80_11855"
     CDS             complement(6123..6527)
                     /locus_tag="PLX80_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21530.1"
                     /translation="MSKVIYTIQFLIILCLVSASDSKSQDKNIDSSNLSKEITEADTN
                     LQNKLFILYQNEETESNEVILIKFNLIKAAVVILNVIDSNGKVIDYLVDGEMLPGTYC
                     VHFKPTEITALKDFSYKIEVNGVTETRKISEQ"
BASE COUNT         2193 a         1141 c         1152 g         2235 t
ORIGIN      
        1 aaaaatatta aagccggcag atttttctac cggcttgatt aaatcttcaa tgtttcccga
       61 agtgacgcct taggagcaat tattattctt cattcaattt aaattccatt aatacagctg
      121 caaattaatt tcaaacaatt tataatttaa tatttaatat ttaatattaa attacggtgt
      181 tattttccca acaaaattta acgcattatt atcagctata gacgcatcag caagatcaac
      241 taaatcatct cctgtcacat ccgaaacgac atatccgctt ccaaaattaa atgcatcatt
      301 gtctataaat tgattgtcgt ctatatcaat gatcccgtcc tgatttacat caccgctata
      361 caggcagaac ttagagcttt tttgaaccat attatttccg aatgcctttg taatattgtc
      421 agtgaaatca taattaagtc cggtacccag tgtataagac tgcggcgttt tgctccatgt
      481 ttcaatcgag tttctgtgct ttgtctgaag ataatacgtt cctgttccgg cattggaaaa
      541 aagaaaactt cccgtatatg taactgagtc aattattgat tttgctgaat caactattgc
      601 atatggtgaa gatgaatttc ttaaatatac tcgtgccgta tcacgcatat taagtcttgt
      661 tgtcggcaca ttataaaaac cttccattat taatttaata gttgacacaa ctgcggatgc
      721 tgaacttgct gaccatcggg agtaagcatc aatacctgat aatgtaatat tattggcaac
      781 tgtattgaca gttccgccgc gagcaaccca gcttgatcct gtgtttgttg atctgaacaa
      841 agttaaagca ggttcaactt ttccgtttaa ttcggattca tcatatttga aaactaatgt
      901 tgcattaagt cctgtgtttg tcgagggtgt aatgtcaaaa tatcttaaaa tgcttgtatt
      961 accggataag ccgttctgcg gagtatgacc tcttttgatc tcagtgcttc caagatttga
     1021 ggcagttgta agaattgatc caaaccctgc aacattcaaa gaacttggtg cattaagatt
     1081 tcgggtagta attatgtagc ctgaatttcc cttaactgtg ttacctggag tttctgtaag
     1141 atagcctgtt ggagaaaccg taatgacatt accgtttaga tcaagatcac caattatcac
     1201 cgaaagagtg tcattaacgg gaacatttgc aagaagtact gttccggtag gattgttaat
     1261 acgtaatccg taataagtga tgtttgctgc agatatattc tgtacatcgg atccgttata
     1321 ttcaattgtg cttccggtat ttgagagcgt tccgttttga attattggat ttgatgcagt
     1381 gaattttgct gtgttactac ccataaggaa taccgcgccg gtattaatgt tcgtactatg
     1441 aaactgatgc ccgcttgtca atgtaacgac tgaaccggaa attatatttg ctgtggtgga
     1501 ccatgaacct gttccagaca atgttgaagt tgaatcaaat ttaaattcag gagttgcaac
     1561 tgatccgtta tttataaata ctgctccaaa aaacacaacc gtagaacctg cgcctgtcat
     1621 tgttccgtta tttgtaagat taccataaat agatactaca tagacgacag agtttcctga
     1681 ataaaatgtt gcgccggcgt tgataactac atttccgttt accctgcaag tgtaaggatt
     1741 agtggttgca gctacggatg cggttcctga aaagacattt aaattagcgt taaagtttga
     1801 gcccgctctt ggatcaattt gcacagtatt ctgagtcctt actgtacctg aatttacaac
     1861 tgtccctccg cttaaaatcg caaatctacc cgaactaaat gtgaagataa aaccattcgc
     1921 ggtaaatatt ccacctgttt ttatttcaaa caaggaacct gtccctgcgg gagaaaacgt
     1981 tacatcattt aatagtgtga catttccatt agaacttata tgaatttcca gtcctgtata
     2041 agtgccatta ccggaaacag tgcaggtcga atcgaatgta aaataaggtg atgcaattga
     2101 acctgtattt acaagtactg ctccaaaaaa cctaaccgaa gagcccgcac ctgtcattgt
     2161 tccgttattt gttaaatttc cataaatcga tactgtatat acggaggagt ttcctgaata
     2221 aaatgttgcg cctgagttta taactacatt tccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnatacgga ggagtttcct gaataaaatg ttgcgcctga gtttataact
     2401 acatttccgt ttaccctgca agtgtaagga ttagtggttg cagctacgga tgcggttcct
     2461 gaaaagacat ttaaattagc gttaaagttt gagcccgctc ttggatcaat ttgcacagta
     2521 ttctgagtcc ttactgtacc tgaatttaca actgtccctc cgcttaaaat cgcaaatcta
     2581 cccgaactaa atgtgaagat aaaaccattc gcggtaaata ttccacctgt ttttatttca
     2641 aacaaggaac ctgtccctgc gggagaaaac gttacatcat ttaatagtgt gacatttcca
     2701 ttagaactta tatgaatttc cagtcctgta taagtgccat taccggaaac agtgcaggtc
     2761 gaatcgaatg taaaataagg tgatgcaatt gaacctgtat ttacaagtac tgctccaaaa
     2821 aacctaaccg aagagcccgc acctgtcatt gttccgttat ttgttaaatt tccataaatc
     2881 gatactgtat atacggagga gtttcctgaa taaaatgttg cgcctgagtt tataactaca
     2941 tttccattta ccctgcaagt gtttggatta ccgtttgacg taacagtggt tgttcctgaa
     3001 aagacattta gatcagcatt aaagtttgag ccggttcttg gatcgacagt cacagtattc
     3061 tgagtcctta ctgtacctga atttacaact gtcccaccgg ttaaaatcgc aaatctaccc
     3121 gaactaaatg tgaagatatt actattaggc gaaaatattc cgcctgtttt aatttcaaat
     3181 aaactttctc ctccattggg agcaaaaacg ccattacttg ttagagttac atttccatta
     3241 ggatttatat atatctgact actttttaaa gtaccaatgc cggaagcaga ggtggtagaa
     3301 tcaaatgtga atatatacgg ctcaatatta ccgctgtttg acaaagatga tccgtaaaac
     3361 ttcagatgcc cccaacctgt aattgtaccg ttaactgtca gacttccttt aataattaaa
     3421 gtatatatga ctgattcacc tgtccagaga ccggctccgg catcgattat tacgtttcca
     3481 tttaatgtgg cagtataaga ttggtaatta cgaacatcaa gtattcccga attaactctt
     3541 aatattgcgc tgaaatttga accgtttaat ggatacagat ttccccctat tgagaattga
     3601 aatgttcctg caccggtgac agaagtacct gagtttaatg ttaatgttgt aatgggatat
     3661 gttaaaacta ttccgctatt tattgagata gtccccatta ggtttaactc actgactgat
     3721 acatttgctg ttaccgtcac agtatggttg attgtagttt gactactgtt tgcagaagtt
     3781 ggagtgcttg atgcagacat ccatgttgct ccgccgttgg ttgagacttc ccaggttgag
     3841 tttgcattcc agtttcctga tgcgattgat ctgtacagct gagcatctgc tgaactgctg
     3901 attagaacca taaataaaaa cagtcctgca aaacatttta gaaatttttt catttaatct
     3961 cctttctgaa gagtttttta tttttaaatt tatttattga aatttactgc tcaaaattat
     4021 attccggatt tattatttta attatccgaa ttcattaaaa ctttctattt aaaatttaac
     4081 atttaaaatt tgaaattaaa aagatacccc tcaatacttt gtgatattaa cttaatcttt
     4141 accaagttaa gtcgtatggc gtacgatgaa tgataataga tttgtatgtg agaggtatct
     4201 gtttaaggtc tgtatattta ctcatcattt acgccattta aacaaaaata aaattaacgt
     4261 caatgtttgt caaagaaaac aatttcggga tttagaacat acgatttaaa ccaattgtaa
     4321 tattatgtgc aaaaaccttt aataactcaa acattaaagc tattttgaaa tcaatatttt
     4381 agcagatata aatagttgat atgcaaaaca caaaaataat taagtttctg aaaactctta
     4441 atcagtatga aattacgcag tttaaagatt ttgttaactc acctgcattc aataaaaata
     4501 aaaatgtaat taaactgttt gatgaactga aaaaatttta tccaaaattt aagaatgatg
     4561 atctgtctga ggaaattatt tacggaaaag tgtttccagg ggagaagttt gattatttta
     4621 aattaagaaa tgcaacttct gatctgctcg tgctcggtaa ggaatttttt tcttacatta
     4681 aattcaaaaa gagcaatata aaggatttat ttctgcttga agaattgagg aaaagaaatc
     4741 tcgataatat ttacgaacag acatacaaag ctgcctttaa gcagaatgaa gaaataaagg
     4801 ttaaggatga aatatatatg tatcacaaaa tgaatctgac tgaagagtta ctttcattta
     4861 acgttatcaa aaaaccgaat gcaatttttc atttccagca ggacatgctt gatatcttca
     4921 taaggtattc tctcattaga ttcttaaagt tttatgatgt catgcttcat gaaggcgggc
     4981 aaaataatta taaatatgaa atgggaatgt ttgaagaggt actaaaatac ttaaagaata
     5041 ataaaatcga tgataatccg acattactgg tctactataa tattattttg ctgatgaagg
     5101 aaaaaaagga taaaaattat tatgagctga agagtcttaa aacaaaatat ataaatgaac
     5161 tcagtgcgcg cgacaggtat atgacatgga ttcacatgaa cagttactgt gtatcgatgt
     5221 ttaacaactg ctccaggacc gatctgctca gagaacagtt tgaacttatg aaagaaaaca
     5281 cagaagatga taccaggaaa cttggcaaga tcctttatcc ggatttttta aatgaagtga
     5341 aaatagctgt cagagttaat gaatttaaat gggcagaaga ttatattgtc aggtttagtg
     5401 agaagcttga agtggaaaaa gaaagcaccc tgaatttctg ttatggttat atcagctata
     5461 agaaaggaga tcttgatgaa gctctgaaat tattttcgaa aacatatttt actttattta
     5521 ttataaaagt tcaggtcaaa atattgcagc ttcagatctg ttttgaaaaa gagtattttg
     5581 accaggcagt ttcattgata gattctttca gacattatct gaaacgggaa gtaactataa
     5641 aggaaatttt cagaatgtct ttttatgaat ttctccgcct tacaaacgag cttatacgat
     5701 taagaactaa ttattacgga agtgataatg atttctataa agaaaagctt aaaaatgaaa
     5761 ttagtaattt gaaaaataat cagtttggga taaaactgtg gctgaaagaa aagttgaacg
     5821 ttgaaagcag atagttttgt caatcggcaa tagttatcgt gaataataca tttttcaact
     5881 gcaattatca tttatcaatc cccaatccca aatcaaaggc actaccccct atccctgcaa
     5941 aattaaaagt aattgaattg ccttaaaacc ttcccatttc cttcacgggg ggaattcgat
     6001 gggaaggcag gcagtaaaaa tattaacccg tcataccggt gatgtcatta aggaaaaaat
     6061 gttcgcgtac atttttccgg gttactattt tattttaatg aacagttgga aattttgaac
     6121 atttattgtt cagatatttt tcttgtttca gtcacgccat ttacttcaat tttatatgaa
     6181 aaatctttta aagctgttat ttctgttggt ttgaaatgta cgcagtatgt accgggtagc
     6241 atttcaccgt caacaagata atctataact tttccattgc tgtcaattac atttagaatt
     6301 acaacagctg cctttatcaa attaaactta attaaaatta cttcatttga ttcagtctct
     6361 tcattctgat aaagtataaa aagcttattt tgcagatttg tatctgcttc tgtaatttcc
     6421 ttagataaat ttgatgaatc aatatttttg tcctgtgatt ttgaatcact tgctgatact
     6481 aagcacagta taatcagaaa ctgaattgtg taaattactt ttgacatttt ttctcctttg
     6541 ggttaggttt ttaattaatt gatacaaaaa tatatcagaa tgattcttaa tccaaagaaa
     6601 ctttttggca ggtttcataa ctcatataaa taatatatct gaataaaatg cctgttaaga
     6661 gctaaatttg aaacatattg catattcaaa gacagatttc ttctaaattt cagggtgttg
     6721 tatatttgtt ttacaattaa tattttgtat ctcaattatt taaaaatatg cataaaagct
     6781 cattactcga gatcatccga acattcactc caaaagaaat a
//
LOCUS       DAORVQ010000177         6636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7260_length_6636_cov_5.855797, whole genome shotgun sequence.
ACCESSION   DAORVQ010000177 DAORVQ010000000
VERSION     DAORVQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7260_length_6636_cov_5.855797"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..808)
                     /gene="truB"
                     /locus_tag="PLX80_11860"
     CDS             complement(119..808)
                     /gene="truB"
                     /locus_tag="PLX80_11860"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQY21531.1"
                     /translation="MNSESTELPFSEFSNKIILADKPLDLTSADVVRIFKRNFSIKKI
                     GHSGTLDPRATGLLILCTDQKTKTINEIIEYDKEYEGTIRIGAVTKSFDTETEEENVA
                     EKFDVSDEQIENVRNTFLGETEQIPPMYSAIKHKGKPLYKLARKGREIKREPRKIIIT
                     DFEVKRLSETEIYFKISSSKGTYIRTIANDFGERLGIGGYLKTLRRLRVGKFDLVGLE
                     DEIEGIMYRLL"
     gene            complement(833..1246)
                     /gene="rbfA"
                     /locus_tag="PLX80_11865"
     CDS             complement(833..1246)
                     /gene="rbfA"
                     /locus_tag="PLX80_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQY21532.1"
                     /translation="MSVRTEKVAQEIKNKLNTAMSKDLSEIGGLGLVTVSKVIVTPDL
                     KMAKIYLSFLGNQIPIDECLEKIETKKKHIRYLLAKIIVMKYMPELFFYHDDTIEYAD
                     KIQKLINDTQEKDKSINKDRAPESEFESDSEPDSD"
     gene            complement(1294..4164)
                     /gene="infB"
                     /locus_tag="PLX80_11870"
     CDS             complement(1294..4164)
                     /gene="infB"
                     /locus_tag="PLX80_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQY21533.1"
                     /translation="MKKVIALVKEIKVSKDVIIDYLKTVGIEASINTALDDETVSKVY
                     SNFRKEIELEDKKMEKSVKFVEKFHVELSDAQEKIEKGNEEKRLEEEKERLKKVIEEE
                     QRAKEDERRKQEILAYKDREKSLKDQEKRDKEKRAAKKADSLKQKTDKKPFAPAVKTI
                     ESKPTHLDNKPTQIDNRPTHIDNKPKQIDNKPKQIDNKPKPVESKPFEKPGDRKTGPV
                     KTFKKPFDKFKKRKPTEKITMDQVLKEKRKQKSFQKPETTASTDPKKEVKKEGFVKRN
                     VTGRLLEDRKFRDKKDRDKKKFETESERKRRVKLEKGHRVKDATQKEIDEAIRDTYAK
                     IEGDTGTSARSIARKRKKKERIEEEKKILEQKEATKNIIKVNEYLSTAELANLMNIDP
                     NEIIKNCFKLGIMVSINQRLEQDIIELIASDFGFKIEFLKDYEEEAIEEEPDMPGDLI
                     DRAPVVTIMGHVDHGKTSLLDYIRKTNVVAGEAGGITQHIGAYKVHMDSGKEISFLDT
                     PGHEAFTAMRARGGQAADIVVLVVSADDSVMPQTIEAINHARAANVPIVIAINKVDKP
                     DSNPDRIRQQLSEKDVLVEEWGGKYQSVEISAKMGMNIDQLLEKILLEAELLELKTNP
                     KKTARGVVLEAKLDKGRGPIATVLVQKGILRVGDSFIAGVYSGKVKAMFDEREHKIEE
                     ATSSSPVVVLGFDGVPQAGDTFVVLGSDKDVKNLSLKRQQLKREQDFRQVKFVTLDDI
                     SKQIQEGKRVELKIILKADTDGSAEALADSLHKLTTNETKVYVIHKAVGSISESDILL
                     AEASDAIVIGFNVRPNLKARKLAEKNNIDVRLYNIIYNLIDEVKNALEGMLAPDISEE
                     VTATVEIREVFKVPKIGNIAGCYVQDGKINRNNKVRLLRDGFEIFKGGISSLKRIKDD
                     VKEVDSGYECGIGLENFNDVKIGDIIEAYKVVETKRKLVTPQ"
     gene            complement(4362..5783)
                     /gene="nusA"
                     /locus_tag="PLX80_11875"
     CDS             complement(4362..5783)
                     /gene="nusA"
                     /locus_tag="PLX80_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQY21534.1"
                     /translation="MKSDIVEAFSQMAKEKNIDRDILSSVIKDSFNKMMEKKYGPEAN
                     FDVVVNMDKGVIEIFLYKVVVEEVMDEGIEIDVETAKEKAGEDFQIGDDFAEEIPIEQ
                     FGRRMVINLKQNLNQKIREIEKEITYNAYKDQVGEIVVGEIYQIKPTSILLIHNGNEI
                     IFPKGEQIPKEKYKKGDTTRAIIKSIEKRQSGPPLIIVSRADEQFLDKLFELEIPEIY
                     DGIIEIKGIAREPGERAKISVISNDDRVDAVGACVGMKGIRIHSIVRELSNENIDVIN
                     YTEDPALYIARALSPAKLNDIYLDNENKIAKIYADEDQISLIIGKSGQNIKLASRLSG
                     YQIDVVREAEEEVEDEEDIDENELDENVNDEVDDEDTDESGEDAEDEADEDAEDEADE
                     KADEEETEEEDSDEDTDDAEEDADENDEQENEIEETEEGLEDAEDSEEATDEELEDAD
                     KLDEDELDAEEDAAEEEETKEKE"
     gene            complement(5808..6278)
                     /locus_tag="PLX80_11880"
     CDS             complement(5808..6278)
                     /locus_tag="PLX80_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21535.1"
                     /translation="METEIKEIAENYFSKSEYKIIDYALRGERGTKVLEIFVDNKTGI
                     NIDELVRINHELDDIIEEKVVLMDISKMVISSPGTERSFKFIWQLLKHKDRDLEIILI
                     NDEQLTGKLTLISEDENKIGVESSIKEKGLKKIRTEYREINFSDIKESRIKLKF"
BASE COUNT         1681 a         1413 c          881 g         2661 t
ORIGIN      
        1 agagctcgct gagagataca catcagactt ttgtttgatc tgaaacagag tttcattttc
       61 aagtgcttta ccaaacggga gtttggtaac gagcgataac tgagattgaa ttttaatttt
      121 acagtagcct gtacattatt ccttctattt catcttccag tcccacaagg tcaaacttcc
      181 ctactctcag tcttcgcagt gttttcagat atccgccaat tccgagtctt tccccaaaat
      241 catttgcaat tgttcttatg taagttcctt tactgctgct gatcttaaaa taaatttcag
      301 tttcgcttaa tctctttact tcaaaatcag ttatgataat tttcctcggc tcgcgtttta
      361 tttctctgcc tttgcgggca agtttgtaaa gcggtttgcc tttatgttta attgctgaat
      421 acatcggcgg gatctgctct gtctctccga ggaatgtatt tctcacattt tcgatctgct
      481 catctgatac atcaaatttt tctgcaacat tctcttcctc agtttccgtg tcaaaacttt
      541 ttgtcactgc gccaatccgt attgttcctt catactcttt atcatattca atgatctcat
      601 taatagtttt agttttctga tctgtgcata agatcagcag acctgttgct cttggatcga
      661 gcgtacctga gtgtccgatc ttttttatgg aaaaatttct tttaaagatc ctcactacat
      721 cagcacttgt cagatccaga ggtttatcgg ctaaaattat tttattggaa aattcactga
      781 agggaagttc tgtagattca gaattcattt attgttttta ttggggaagt tcttaatctg
      841 agtcaggttc agagtcagat tcaaattcag attccggagc tctgtcctta ttaatacttt
      901 tgtctttttc ctgagtatca tttataagct tctggatttt atctgcatac tcaattgtat
      961 catcatgata aaaaaacaat tcaggcatat atttcattac tataattttt gcaagtaaat
     1021 atcttatgtg cttctttttg gtttcaatct tttcgagaca ttcatcgatc ggaatctgat
     1081 ttccgaggaa gctgagataa atttttgcca tctttagatc aggcgtaact attacttttg
     1141 aaactgttac aagtccaagt ccgccaatct ccgacagatc ttttgacatt gctgtattta
     1201 atttgttctt tatttcctgt gctactttct cagttcttac tgacatgcta atatactttt
     1261 actttttctc tttttataaa atctaaatta aatctattga ggtgtcacaa gtttcctctt
     1321 tgtttcaact actttatatg cttctattat atcgcctatc tttacatcat taaaattctc
     1381 aagaccaatt ccgcattcat aacctgagtc aacttctttt acgtcatcct tgatcctttt
     1441 cagtgaagag atacctcctt tgaaaatttc aaatccgtct ctcaaaagtc ttaccttatt
     1501 attcctgttg atctttccat cctgcacata acatcctgcg atatttccga tctttggaac
     1561 tttgaatact tctctgattt ctactgttgc tgtcacttct tcagaaatgt ccggagcaag
     1621 cataccttca agagcatttt tcacttcgtc tatcaggttg tatatgatat tgtataatct
     1681 tacatcaata ttatttttct cagcaagttt tcttgctttc agatttggtc ttacattaaa
     1741 tccgattaca attgcatctg atgcttctgc aagaaggata tcagattcac tgatagaacc
     1801 aactgcttta tgtatcacat aaacttttgt ctcatttgta gtaagcttat gaagtgaatc
     1861 cgcaagtgct tctgctgatc catcggtatc tgctttaaga ataattttta attcaactct
     1921 cttaccttcc tgtatctgtt tggaaatgtc atcaagcgta acaaacttca cctgtctgaa
     1981 atcctgttcc cttttaagct gctgtctctt caatgaaaga ttctttacat ctttatcaga
     2041 tccaagtaca acaaaagtat cacctgcctg cgggacaccg tcaaatccaa gtactacaac
     2101 cggtgatgac gaagtcgctt cttctatctt atgttctctt tcgtcaaaca tggcttttac
     2161 tttaccgctg taaacacctg ctataaaaga atcaccgact cttaatattc ctttctgaac
     2221 aagtacagta gcaataggtc ctcgtccctt gtctaacttg gcttccaata caacacctct
     2281 tgcagttttt ttcggattgg ttttaagttc aagcagttca gcttctaata gaattttctc
     2341 aagtaactga tcaatgttca ttcccatttt cgcagagatc tcaactgatt ggtacttacc
     2401 gccccactct tccaccaata catctttttc agaaagttgc tgtctgattc tgtcaggatt
     2461 tgaatccggc ttatcgactt tatttattgc aatgacaatc ggtacatttg ctgctcttgc
     2521 atgatttata gcttcaatgg tctgaggcat tacactatca tcagctgaaa ctacaagcac
     2581 aactatatcc gcagcctgtc cgcctcttgc tctcatagct gtaaatgctt cgtgtccggg
     2641 agtatcaagg aaacttattt cttttcccga atccatatga actttataag caccgatatg
     2701 ctgagtaata ccacctgctt cacctgccac tacatttgtt tttctgatat aatcaagaag
     2761 tgatgtctta ccgtgatcca catgtcccat gattgtaacc actggagctc tgtcaataag
     2821 atctcctggc atatccggtt cttcttcaat agcttcctct tcataatctt tcaggaattc
     2881 aattttaaat ccaaagtcac tggcaatcaa ttctataata tcctgttcaa gtctctgatt
     2941 tatggaaacc attattccga gtttgaaaca attttttatg atctcattcg gatcaatgtt
     3001 catcaggttt gcaagctctg ccgtagataa atattcattt acctttataa tattttttgt
     3061 tgcttctttt tgctcaagta tttttttctc ttcctcgatc ctctcttttt tctttctctt
     3121 tctggcgata cttcttgctg atgttccggt atctccttct atctttgcat aagtatctct
     3181 gatcgcttca tcaatttcct tttgagtggc atctttaact ctgtgccctt tttctaattt
     3241 gactcttctt tttctttctg attcggtttc gaatttcttt ttatccctgt cctttttatc
     3301 tctgaacttt ctgtcttcaa gcagtctgcc tgttacattt cgttttacaa aaccttcttt
     3361 tttaacttct ttttttggat ctgtggaagc agtagtttca ggtttttgaa aagatttttg
     3421 tttcctcttt tcttttaaaa cctgatccat tgttattttt tcagtaggtt ttcttttttt
     3481 gaatttatca aaaggctttt taaaagtttt taccggtccg gtctttctat cacccggctt
     3541 ttcaaaaggt ttactttcta caggcttagg tttattgtct atctgctttg gtttattatc
     3601 tatctgcttt ggtttattat ctatatgcgt tggtctatta tctatttgtg ttggtttatt
     3661 atctaaatgc gttggtttgc tctcaatagt tttgactgca ggagcaaaag gtttcttatc
     3721 agttttttgt tttaatgaat ctgccttttt ggctgctctt ttttctttat cccttttttc
     3781 ctgatctttt aaactctttt ctctgtcttt ataagcaagt atttcctgtt ttcttctttc
     3841 atcttcttta gctctttgct cttcttctat aacttttttt aatcgttcct tttcctcttc
     3901 gagtcttttt tcttcattac ctttctcgat cttttcctga gcatctgata gttccacatg
     3961 aaatttttct acaaatttca cagacttttc catcttctta tcttcaagct ctatctcttt
     4021 cctgaagtta gaatatacct tgctcacagt ctcatcatcc aatgcagtat taatagatgc
     4081 ttctattcct accgtcttaa gataatcgat tatgacatct ttcgatactt ttatttcttt
     4141 tactaatgct attacttttt tcatataatg ttaaaatttt taccgggaca aaattctgat
     4201 cccggaaatt ccggttatgc tcagtcgacc gtttaaatct gttaatatct atttatttca
     4261 ttgtactctt taatttaaat tgctgaaatt aattttctat tccagatttt tttataactt
     4321 ttttgaacca aagtttatcg gaatgaaaga aagttagagc cttattcttt ttcctttgtc
     4381 tcttcttctt cagcagcatc ctcttccgca tccagttcgt cttcatctaa tttgtctgca
     4441 tcttcaagct cttcatcagt tgcttcttct gaatcttctg catcttcaag accttcttct
     4501 gtttcttcaa tttcattctc ctgctcgtca ttctcatcag cgtcttcctc agcatcatct
     4561 gtatcttcat ctgaatcttc ttcttcagtt tcttcttcat cagctttttc atctgcttcg
     4621 tcttcagcat cttcatctgc ttcatcttca gcatcttctc cagattcatc tgtatcttca
     4681 tcatctactt catcgttcac attttcgtca agttcatttt catcaatatc ttcttcatct
     4741 tcaacctctt cttcagcttc tcttactaca tctatctgat atccggaaag tcttgaagca
     4801 agcttaatat tctgtccgct ctttccaatg ataagactta tctgatcttc atctgcatat
     4861 atttttgcga tcttattttc attatcaaga taaatatcat taagttttgc aggagataaa
     4921 gctcttgcaa tatataatgc cggatcttct gtataattta ttacatcaat attttcatta
     4981 cttaattctc ttacaataga gtgaattctg attcccttca tccctacaca tgcgcctact
     5041 gcatctactc tgtcgtcatt agaaattact gagatctttg ctctttcacc aggctctctt
     5101 gcaattcctt ttatttctat aatgccgtca taaatttcag ggatctcgag ttcaaataat
     5161 ttatcaagaa actgttcatc agctctggat actatgatga gcggcggacc tgactgtctt
     5221 ttttcaatac tcttaataat tgctcttgta gtatcacctt tcttatattt ttctttaggt
     5281 atctgttcgc cttttgggaa aatgatttca ttaccattat gaatcagaag tatcgaagtc
     5341 ggtttgatct gatagatctc tcctactaca atttcaccaa cctgatcttt ataagcattg
     5401 taagtgattt ctttttcgat ctctcttatc ttttgattaa gattctgttt cagatttatc
     5461 accattcttc tgccaaattg ttcgatcggt atttcttcag caaagtcatc cccaatctga
     5521 aaatcttctc ctgctttttc tttagcagtc tcaacatcga tctctattcc ttcatccatc
     5581 acttcttcta ccactacttt ataaaggaat atctcaatta cgcctttatc catatttacg
     5641 acgacatcaa agtttgcttc gggtccatat ttcttttcca tcattttatt gaacgagtcc
     5701 tttatcacac ttgaaagtat gtctctgtca atgttttttt ctttcgccat ttgtgaaaag
     5761 gcttctacta tatccgattt catattatgc tgctgatttt tattatttta aaattttaat
     5821 ttaattcttg attcttttat atcgctgaaa tttatttcac ggtattctgt ccgtattttt
     5881 tttaaacctt tttcttttat cgagctctcc acaccgatct tattttcatc ctcagagatc
     5941 agtgtaagtt taccggtcaa ctgctcatca ttaataagaa tgatctccag atccctgtcc
     6001 ttatgtttca ataactgcca gataaattta aatgaccttt cagttccggg ggatgagata
     6061 accattttag agatatccat caatacaact ttctcttcaa tgatatcatc aagctcatga
     6121 tttatcctta ccagctcgtc tatgtttatg cccgtcttat tatccacaaa tatctcaagt
     6181 accttagtcc ctcgctcacc tctcaatgcg taatcaatta tcttatattc agatttactg
     6241 aaataatttt cagcaatttc cttaatttca gtttccattt tttgtaaaaa aaaagtgggt
     6301 tcgacaaacc caccaatata tattcatttt aaatgcctga caaaatactt tttatatgat
     6361 aaaatttcaa ggcagagatg aaaaagcaat taaaaattaa aaattacgaa ttaaaaattt
     6421 agaatggtaa tttagaatgg taatttagaa ttaattattt agaacctgaa tacctatcaa
     6481 aatggtaaaa aaattttggc gaattttcaa gaaaaaaatc taaactttta atagcacgat
     6541 tttaaataat tcacaattaa tttcccctta aaaaaggctg tctcaaaaca ctatttttac
     6601 cactaagtca caaagacaca aagaaaaaaa ctttaa
//
LOCUS       DAORVQ010000178         6632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7271_length_6632_cov_6.075110, whole genome shotgun sequence.
ACCESSION   DAORVQ010000178 DAORVQ010000000
VERSION     DAORVQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7271_length_6632_cov_6.075110"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1153
                     /locus_tag="PLX80_11885"
     CDS             386..1153
                     /locus_tag="PLX80_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21536.1"
                     /translation="MISELPMYVSFIFNAAVILTAVFFYFAVRSKPKLNFEDGIDPDM
                     NINIENDSRVDTETDYSKITTIVMLIFLLTQGVLGMTGFFIDTNAIPPRMIYLAPPAL
                     LFLFGIFFLNSGKKFTDSLNPKILTYLHIVRIPVEIVLYLLFVNKLVPEVMTFAGNNF
                     DILAGISAPFAAYFGYRKNPESKIARTGLLIWNFICLGLLFNIILTAVFSVTSPFQLL
                     AIKQPNIGMLYFPFMWLPGFIVPLVFFSHLICIRKLL"
     gene            complement(1395..1778)
                     /gene="rnpB"
                     /locus_tag="PLX80_11890"
     ncRNA           complement(1395..1778)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLX80_11890"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1823..2254)
                     /locus_tag="PLX80_11895"
     CDS             complement(1823..2254)
                     /locus_tag="PLX80_11895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HQY21537.1"
                     /translation="MEKFVKVFTDGASRGNPGMSAIGIAVYNHEDELIVDHKKFIGTG
                     TNNSAEYKALIESIKLLSSISHDFDSVNFYCDSELVVKQIKGQYKIKNPDMIFLSNEF
                     FKELSSLQKKYTITHIPREKNKTADNLANQALDELKKNKTL"
     gene            complement(2261..3166)
                     /locus_tag="PLX80_11900"
     CDS             complement(2261..3166)
                     /locus_tag="PLX80_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQY21538.1"
                     /translation="MTENLNTGISIEISERVKIMQNDQEKSSLENDDNESFDSNNDES
                     SSVFHTFDSYEDESEVPKRTELLKNFLKLREIDEELTDIEEEKGDLPFEINSIKEKIK
                     NLSGSIDARKERLSNLTDEVNSLKDENSSFEDRISKYDEQKFNVRSNTEYDEITKAIE
                     GLFDTVEKNEKKIKEINKEIDGLSENSDKNENLLKELSEDLGEKESQLNELNDQFMQD
                     ESALRDKRAKLIEKLEPKYVQLYERINGNYKGEATAIVRNGNCSGCYNSIPPQRVIEI
                     KTAENIYTCQSCGRILISEHLKMDD"
     gene            complement(3163..3777)
                     /locus_tag="PLX80_11905"
     CDS             complement(3163..3777)
                     /locus_tag="PLX80_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21539.1"
                     /translation="MKKIFSKSEIKSEENFHQIIKKKKVIVFVPQSHLEKVFSAMADA
                     GAGKIGNYDQCSFRINGTGSFRPLKNTRSFSGKKNVLSLENEIRLEMECNAENVSSIV
                     NAMLKSHPYEETAYEIYDYVKFDKKITGKIIELNRKTKLKVILNRLNKKIISETETFE
                     RVIKKVVITESSFTDKIKISAEYFDSDLVLICRNNKYKIFNLLQ"
     gene            complement(3990..4940)
                     /locus_tag="PLX80_11910"
     CDS             complement(3990..4940)
                     /locus_tag="PLX80_11910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQY21540.1"
                     /translation="MKVIEHLEKAKNPLISFEILPLQRGGDINVLFESLDALMKFNPP
                     YIDVTSHSAQVQYEETPAGIKKKVKRKRPGTIGISVAIQNKYNIDTVPHILCNGFTKE
                     ETEDALIELNYLGIENVLAVRGDELGFKKPIPEGRTSNIYASDLVDQIAHMNSGKYLE
                     EDLLDAAPTNFCIGIGGYPEKHFEAPNLNTDIKIAKDKIDLGAHYIVTQMFYNNKYYF
                     EYVDKCKSAGINAPVIPGLKILTSKNHLTSIPKNFFVEIPDELSGEVQEAKPEHVLDI
                     GVEWAFKQSEELLNSGVPSLHFYIMQSSKPIEKLMKKLKL"
     gene            complement(5378..6367)
                     /locus_tag="PLX80_11915"
     CDS             complement(5378..6367)
                     /locus_tag="PLX80_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21541.1"
                     /translation="MQRINLILIIILITFSSTFAQNKKIAYCSNQGTSEFLQVFTMND
                     DGTNKKQLTNLEENCMRPKFSPDGKQIVFYSDKGFVYLIRDVDNPDLNRTFLVWDGMY
                     PTFMPDGNEIMFNSEDDDDVLSIYVIDTAEIGAKPQIVTDGSYANQQILSSDGKTLAY
                     SSFAGGTKAIMVANLEDESDNYISQASVNNDANLEPDFSSEGNMLVYASFDSNLNGTI
                     RILIDGNESAISKGITSANVPRFSPDGKTIAFAVITGSNVDLYTMSPDGSNRRNLNIS
                     GGDLGTFQWVNNETIVYDAGSDTRLSVGLVDVGSGSSEILAEGGFNLHPSTVK"
     gene            complement(6523..>6632)
                     /locus_tag="PLX80_11920"
     CDS             complement(6523..>6632)
                     /locus_tag="PLX80_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21542.1"
                     /translation="SYEVSFNAASLSSGVYFYKLESGDFIDTKKMFLLK"
BASE COUNT         2037 a         1188 c          971 g         2436 t
ORIGIN      
        1 caacttactt tttttgaatg actcccctct gaaagcataa ggggacaagc tccaaaagta
       61 ccaaaaaaag tcgccctctg atgtaaaatt acctaaattc tttgcatcct gaggactcgg
      121 gactataaaa tttccaactc aaaatttgct tcgcaaaatt ctcagacatg aaaattttaa
      181 cagcgcaatt gtttaggatt tttaacggta attttacata gacgggttat ttataaactt
      241 tattaataat tttatattat tatagaaatt tgtactgttt ttttgatgat caatttgttt
      301 tggacattat tttcatagaa tataaatccg gctctacttt actattttaa tatttttatt
      361 tattatttaa aatttaatat tattcatgat ttccgaactt cctatgtatg taagttttat
      421 ttttaatgca gccgttattc tgacggctgt ctttttttat tttgctgtaa gatctaaacc
      481 aaaacttaat tttgaagacg gtattgatcc ggatatgaat attaatattg agaatgattc
      541 gagagttgat acagaaacgg attattccaa aataacaaca atagttatgt tgatttttct
      601 tctgacacag ggagttttgg gaatgaccgg attttttatt gatacaaatg caattccacc
      661 aaggatgatc tatctcgcac cgcctgcatt attattttta tttggtatat tttttctgaa
      721 ttcaggtaaa aaatttacag attcacttaa tccaaaaatt cttacttacc ttcatatagt
      781 gcggatacct gtcgagatag ttttgtatct tttatttgtg aataaattag tgccagaggt
      841 aatgaccttt gccggaaata actttgatat acttgcgggt atatcagctc cattcgctgc
      901 ttatttcggt tatagaaaaa atcccgaaag taaaattgcc agaacaggtt tactgatctg
      961 gaatttcatc tgtctcggct tactgtttaa tattattctg accgctgtct tttctgtaac
     1021 ttcgcccttt cagttgctcg caattaagca gcctaacatc ggcatgcttt atttcccttt
     1081 tatgtggctg cccggattta tagtgccgct ggtatttttc tcacatctta tttgcatcag
     1141 aaaattgttg tgaggatttt aattgtgaaa attgcctcga agactcaaag gctctgagga
     1201 gttgatttag atattatatt caattctgaa atttgaaaga gattgtgttt taaatatttt
     1261 gcttcgaagg actaaaggct caatactttt aaaaattatt aaggcagcct ggataatgaa
     1321 agattaagag atatgacaaa gaattttaga actgagaagt tcttattgaa gtgggtttag
     1381 agtttatcat taaaaaatct attccgttaa gtaagccgga ttctgtttcc ttaaaaagga
     1441 tgacgatcat ttatcttatc tttacattgc tgtaaagctt tttgcaacct accccgggaa
     1501 tataaaaagc ggacagttct tcaattccct atacatggtc ttgctccgga tgaggtttgt
     1561 agatcccgga gatcgctctc cggatggtag gctcttaccc tgccttttca cccttatcgc
     1621 cggttgcccg tgacggtata ttttctgtta cactttctgt aattagctct tgcgaattaa
     1681 ctacccggga ttatctcccg gcatccctgt ccgttgcgga gtccggactt tcctcacatc
     1741 ctgtaaacag gacagtgcga tcgtctataa cggaatagaa tatattaaag aacatttaat
     1801 gacaattcaa actgcagatc ttttataatg ttttattctt tttcaattca tctaatgcct
     1861 gattggcgag attatctgcg gttttatttt tctctcttgg aatatgagta atagtatatt
     1921 ttttctgtaa actactcaac tctttaaaga actcatttga aagaaaaatc atatccggat
     1981 tttttatttt atactgacct tttatctgct ttacgactaa ttcactgtca caataaaaat
     2041 tcaccgaatc aaaatcatga ctaatactgc ttaataattt tatactttct ataagagctt
     2101 tgtattcagc ggaattattt gtacctgttc caataaattt tttgtgatct actataagtt
     2161 catcctcatg attataaact gcaattccaa ttgctgacat tccgggatta cctcttgaag
     2221 ccccgtctgt aaagacttta acaaattttt ccattacaga tcaatcatcc atttttaaat
     2281 gctctgagat aaggattctt ccgcaagact gacaagtgta aatattttcc gcagttttaa
     2341 tttcaattac tctttgcgga ggaattgaat tatagcatcc cgaacagttt ccgtttctga
     2401 caatagcagt agcttcacct ttataattac catttattct ttcatacaac tgcacatatt
     2461 taggttcgag tttttcaata agttttgctc tcttatctct caaagctgat tcatcctgca
     2521 taaactggtc attaagttca tttaactgac tttctttttc accaagatct tcagaaagtt
     2581 ctttcagcag gttttcattt ttatcggaat tttcgctgag accatcaatt tctttattga
     2641 tctctttgat ctttttttca ttcttttcta cagtatcgaa caaaccttca atcgcttttg
     2701 taatctcatc atattctgta ttagatctca cgttaaattt ttgttcatcg tacttactta
     2761 ttctgtcttc aaaagaagaa ttttcatctt ttaaagaatt tacttcatca gtaagatttg
     2821 aaagcctttc tttcctggca tctatactac ctgaaagatt ttttattttt tctttaatag
     2881 aatttatttc aaaaggcaga tcaccttttt cttcttctat atctgtaagc tcttcgtcaa
     2941 tttctctaag cttcaagaaa ttcttaagta gttcagttct ttttggaact tcggattcat
     3001 cttcgtatga atcaaaagta tgaaatactg atgaagattc atcattattt gaatcaaaag
     3061 attcattatc atcattctcc aaagatgatt tctcctgatc attttgcata atttttaccc
     3121 tttcagaaat ctcaattgaa attccggtat ttaaattttc agtcattgta ataaattaaa
     3181 aattttatat ttattgtttc ggcagattaa gacaagatct gaatcaaaat attctgctga
     3241 tatttttatt ttatcagtaa aagaactttc agtaattaca acttttttga ttaccctttc
     3301 gaatgtttct gtttcagata ttattttttt attcaatctg tttagaataa cttttaattt
     3361 tgtttttcgg ttcagctcaa ttattttgcc tgtaatcttt ttatcaaact taacataatc
     3421 ataaatttca taagcagttt cttcatatgg gtgtgatttc aacatagcat ttacaatact
     3481 gcttacattt tcagcattac attccatttc taatctaatc tcattttcta agcttagaac
     3541 atttttctta ccggaaaatg acctggtatt tttcaggggt ctaaaggaac ccgttccgtt
     3601 aattctaaaa gaacattgat cgtaatttcc aatctttcct gctcctgcat cagccatagc
     3661 tgagaacact ttttcaagat gggactgagg gacaaaaact attactttct tttttttaat
     3721 tatctggtga aaattttctt ctgatttaat ttcactttta ctgaagattt ttttcattac
     3781 tggttgaaat taatttacac ataaaaaaaa gtgtatcttt aattggatac actttttttt
     3841 aagctttttc tttaatggaa tcaggctttt tgatttgatt ctgcctgttt tcactgaaac
     3901 ctttatataa aaaatcagat atttacttaa tttcatttgc ctaaaatata cacgttagtt
     3961 tataaaaaca agttactaat aaaccaagat cagagcttta gttttttcat caatttttcg
     4021 ataggttttg aactttgcat aatataaaaa tgaagagatg gcacaccact gtttaacaat
     4081 tcttcagatt gtttaaaagc ccattcgacc ccaatatcca aaacgtgttc cggcttagct
     4141 tcctgtactt caccagagag ttcgtcagga atttcaacaa aaaagttttt aggaattgac
     4201 gtaagatgat ttttggaagt aagaattttc aaccccggga tcactggagc attaatacca
     4261 gcagatttac atttatcgac atattcaaaa tagtatttat tattatagaa catttgagtt
     4321 acgatgtagt gagcaccaag atcaattttg tctttagcta ttttaatatc tgtattcaga
     4381 ttcggagctt cgaaatgttt ttcgggatat ccgccgattc caatgcagaa atttgtagga
     4441 gcagcatcca gcagatcttc ttctagatat ttacctgaat tcatatgagc aatctgatct
     4501 acaaggtcac tggcataaat atttgaagtc ctaccttcgg gaataggttt tttgaatcca
     4561 agctcatcac ctctcacagc aagtacattt tcaataccca gataattcag ttcaatgaga
     4621 gcatcttcag tttcttcttt agtaaaaccg ttacataaaa tatggggaac agtgtcgatg
     4681 ttatatttat tctggattgc aacactaatt ccaatcgtac cgggacgttt acgttttact
     4741 tttttcttta ttcctgctgg agtttcttca tattgcacct gagccgaatg agaagtaacg
     4801 tcaatatacg gaggattaaa tttcatgaga gcatcaagag attcgaagag aacattgatg
     4861 tctccgcctc tttggagcgg aagtatttca aaacttatca gagggttttt tgctttttct
     4921 aaatgttcaa tgactttcat aatttaaaaa cggatagatc cgtttttcac aaacctttct
     4981 attaaattaa aaactaatat taaattgcca atcacagctt tatccaaaag tatttcaaag
     5041 tgaaaactat atttctgaga ctttgaaact tacaaagtca ataaatggat aaatccgttt
     5101 tacacaacta ctttctacct gataataact atacaaaaat tcagattcat cattaactat
     5161 cataattatt caaagtaaag ataactattc tcaagactct tttcctgcag attcaaaaaa
     5221 cggataaatc cgtttttcca aagcctttct aaaaaattaa agactaaaaa aaatctaacc
     5281 cttcttcgat gtctcaattt tttccaaagt aatcaataca tttctatata tttgctactt
     5341 tttaagtaaa aaacggataa atccgttttt ccaaacccta cttcaccgta ctcggatgca
     5401 aattaaaacc accctctgca agtatctctg aacttcccga tccaacatca accaatccta
     5461 cactcagacg tgtatcactt ccggcatcat aaacaatggt ttcgttatta acccactgaa
     5521 atgtccccag atctccaccg ctgatattga gatttcttct attgctgccg tcaggagaca
     5581 tcgtatatag atcaacattg ctgccggtta ttactgcaaa agcgatggtc tttccatctg
     5641 gagaaaatct tggcacatta gcgctagtaa tgcctttgga aattgctgat tcattaccat
     5701 cgattaaaat tcgaattgtg ccatttagat tactgtcaaa gcttgcatag acgagcatgt
     5761 tcccctctga tgaaaaatca ggctcaagat tagcatcatt attgacactt gcctgactaa
     5821 tgtaattatc agattcatct tcaagattag ctaccataat tgctttagtt ccaccggcaa
     5881 atgaagaata agccagggtt tttccatctg aagaaaggat ttgctggttt gcataagatc
     5941 cgtcagttac tatctgaggt tttgcaccaa tctctgcagt gtcgattaca taaatagata
     6001 aaacatcatc atcatcttcg gagttgaaca taatttcatt accgtccggc ataaatgtcg
     6061 gatacattcc atcccatacc aaaaaagtcc tgttgagatc aggattatcc acatctctga
     6121 tcagatatac aaaaccttta tctgagtaaa atacgatctg ttttccgtca ggagaaaact
     6181 ttggtctcat acaattttct tcaagatttg taagctgttt tttatttgta ccgtcatcat
     6241 tcattgtaaa aacctgcaaa aattcagaag taccctgatt ggaacaatat gctatttttt
     6301 tattctgagc aaaagtcgat gaaaatgtta taagtattat tattaaaatt aaatttattc
     6361 tttgcatttt aaagtaagtt gttaaaaata atctgtaaaa taggcaactt aattttctta
     6421 ttaaagagat taaattggtt gtttggaaga gtttggcatt aaataaaaaa gcccggtatg
     6481 ttaacaccgg gctttaatct taataaaaat gttaatatga ttttatttca gtaagaacat
     6541 ttttttggta tctataaaat cacctgactc taatttgtag aaataaacac cacttgatag
     6601 agatgcagca ttaaagctaa cctcatatga ac
//
LOCUS       DAORVQ010000179         6615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7297_length_6615_cov_5.785976, whole genome shotgun sequence.
ACCESSION   DAORVQ010000179 DAORVQ010000000
VERSION     DAORVQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7297_length_6615_cov_5.785976"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLX80_11925"
     CDS             <1..190
                     /locus_tag="PLX80_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21543.1"
                     /translation="DINKDTISLTATHFVYSLPNTNDCEFEVKFYKGFAFIRYTKGYC
                     EGQFGFNASIDGIFLKVN"
     gene            341..880
                     /locus_tag="PLX80_11930"
     CDS             341..880
                     /locus_tag="PLX80_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQY21544.1"
                     /translation="MDFYSLHINTPSGKELSMKDYKGKVVLIVNTATNCKLASQFDEL
                     EKLYIAYKDQGLVIIGTPCNQFNNQEPLENEEMIEACRVDHGVTFQLTEKIDVNGADT
                     HPLYQYLKRESDTFFSKIFGNNIKWNFTKFLINRDGKLVKRYSPTTKPAKIMDKLKTL
                     LNSNPKNCRTKFLRYKAES"
     gene            1049..2137
                     /locus_tag="PLX80_11935"
     CDS             1049..2137
                     /locus_tag="PLX80_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQY21545.1"
                     /translation="MLTIIIICTILTVLVTILPLSHNKHWTIRGMDFPRLQFMIFSLG
                     LLTANLIFLDIGKSLTLIMISLTAICLIWQLWWILPYTPFWSKEVRSCNEANPGRQLS
                     IITSNVLTPNRNSEALIELVRKYQPDVLVTLESDKWWQEQLKVLESEMPYTVKCPLDN
                     LYGIHLYSRLRLHDQEISFLIEKDVPSIHVSIELLTGEMVRAHFLHPAPPSPTENEES
                     AERDAELITVARSVAKSDQPVIVAGDLNDVAWSSTTRLFRKISGLLDPRIGRGMFNTF
                     HAKFALSRWPMDHLFHSNHFTLCSIQRLPSISSDHFPLFTSLAFTPLEGSSQEGIDAD
                     SEDHDRAKEIVNEKGGNVDDVPQPGGNT"
     assembly_gap    2376..2475
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2476..3011
                     /locus_tag="PLX80_11940"
     CDS             <2476..3011
                     /locus_tag="PLX80_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY21546.1"
                     /translation="LNKNNYIILSGAMGAGKTSVIRKLKDLGHACVDEPARQILEEQR
                     KIGGNGLPEKDPKLFTELMLSRTIFQYKLHEKNSEPVFFDRAMPDFIAYSNLFNVDET
                     VFRNAAKEYRFNKTLFFFRGTESIYTTDNERKMSFDQADEFGNNVRKIYRELGYEIHD
                     VPFLDIGSRVEFILEKI"
     gene            3216..3374
                     /locus_tag="PLX80_11945"
     CDS             3216..3374
                     /locus_tag="PLX80_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21547.1"
                     /translation="MTTRYLKINIPEDIQAIKEDGILLVIRSILDTYKNNSNEYFTDD
                     EINLINNS"
     gene            3497..5692
                     /gene="katG"
                     /locus_tag="PLX80_11950"
     CDS             3497..5692
                     /gene="katG"
                     /locus_tag="PLX80_11950"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQY21548.1"
                     /translation="MENNSIESGKCPFTGARNIGSGGRNNTDWWPNQLKINILRQHSS
                     LSDPMGEDFNYAEEFKKLDYAGLKKDLHDLMTDSQVWWPADFGHYGPYFIRMAWHSAG
                     TYRTADGRGGAGGGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGQKISWADLFVLTG
                     NVALESMGFKTFGFAGGREDQWEPDADVYWGSEKTWLGDERYSGDRDLENPLAAVQMG
                     LIYVNPEGPNGNPDPIAAAKDIRETFKRMAMDDKETVALIAGGHSFGKTHGAGEASNL
                     GNDPEGCDIEGQGLGWSSKFNSGMGVDTISSGLEVTWTETPVKWSNNFFENLFKYEWE
                     LEKSPAGANQWVAKDADAVIPNAFDPSKKQLPKMLTTDLSLRFDPEYEKISRRFLEHP
                     EELADAFARAWFKLIHRDMGPKARYLGPEVPTEELIWQDPIPEVDHILINEKDIEELK
                     SIVLNSGLSISELVSTAWASASTFRGSDKRGGANGARIRLAPQRSWTVNNPSQLDKVL
                     ETLEAVHNEFNFDQKNKAEGKKVSLADLIVLAGCAGIEQAAKDASQEVTVPFSPGRMD
                     ASPEQTDAEAFEALEPIADGFRNYQKTKYNVSTEELLLDKAQLLNLTAPEMTVLIGGM
                     RVLKTNFDNSELGVFTKNPGTLTNDFFVNLLDMNTVWKATSEEKDLFEGSDRATGELK
                     WKGTRSDLVFGSNSELRSLAEVYGSSDCKEKFVKDFIAAWDKVMNLDRF"
BASE COUNT         2267 a         1053 c         1272 g         1923 t
ORIGIN      
        1 agacattaac aaagatacca ttagtctgac agcaacgcat tttgtttaca gtttacccaa
       61 tacaaatgat tgtgaattcg aagtaaagtt ttacaaaggg tttgctttta tcagatacac
      121 gaaaggatat tgcgaaggac aatttggatt taacgcttcg atcgatggga ttttcttaaa
      181 agttaattaa tacaattaat acaattttta caattgatac aattgataca attgatacaa
      241 ttgatacaat tgatacaatt ggtaagggta aaatacaaaa tttataaatg tagatatttt
      301 aaatatattt attactgaaa cctttgaata taaaacaaga atggactttt attcactaca
      361 cataaataca ccgtctggaa aagaactttc aatgaaagac tataaaggga aagttgtttt
      421 gattgttaac acagcgacaa attgcaaact tgcttctcag tttgacgaat tggaaaaatt
      481 atacattgct tataaagatc agggactagt aataatagga actccctgta atcaatttaa
      541 taaccaggaa cctttagaaa atgaagagat gatagaagca tgtcgggtag atcatggagt
      601 aacatttcaa ttaacagaaa agatagatgt gaatggagct gacacacatc ctttatatca
      661 gtatctgaaa agagagtctg atactttttt tagtaaaata tttggaaata atataaaatg
      721 gaactttacg aagttcttaa ttaaccgcga tggaaagtta gttaaacgat actcaccaac
      781 aaccaaacct gcaaagataa tggataaatt gaaaacttta ttgaattcaa atcctaaaaa
      841 ctgccggaca aagtttttga gatataaggc tgaaagttaa agtctaaact gatgaaattt
      901 taatatatat aagtttaaga ttggttgatg taattttgcc ttaaataagt aatctcaaaa
      961 ataataaacc gcagaaattt tttcaaaata aaatactcgc taaagcagtg taataaaaaa
     1021 ctcaactaaa aaggaattca aaactaagat gctaacaata attattattt gtacaattct
     1081 gactgtgctg gttacaattt tacccttatc gcacaataag cattggacta ttcgaggtat
     1141 ggactttccc agactacagt ttatgatctt ttcacttggc ttgttaactg caaatttaat
     1201 ttttctcgat ataggaaagt ctttaactct gataatgatt tctttaacag ccatttgttt
     1261 gatttggcaa ttatggtgga tattgcctta tacacctttc tggtcaaaag aggttagatc
     1321 ttgtaatgaa gcaaatcccg ggaggcagct cagcattatc acctctaatg ttctgactcc
     1381 aaatcgaaat tctgaagctt tgatagagtt ggtaagaaaa tatcagccgg atgttcttgt
     1441 tacattagaa tcagataagt ggtggcagga gcaactcaag gtattagaat cagaaatgcc
     1501 atatacagtc aaatgtccgc ttgataacct ttatggaatt catttatatt cgagattgcg
     1561 tttacatgat caggaaattt catttttaat agagaaagat gttccctcga tacatgtatc
     1621 tattgaattg ttaacaggtg aaatggttcg tgcacatttt ctgcatcctg ctccaccaag
     1681 ccctactgag aatgaagagt cggcagaacg tgatgctgag ttgataacgg ttgcccgaag
     1741 tgtcgccaaa agtgatcagc ctgtcatagt ggcaggtgat ctcaacgatg tagcatggtc
     1801 ttcaacaact cgtcttttcc gtaagattag cggcttactg gatccgcgga taggacgagg
     1861 aatgttcaat acctttcacg caaaatttgc gttatcacga tggccaatgg atcatctatt
     1921 ccatagtaac cattttactc tgtgctccat tcagcgttta ccctctataa gttcggatca
     1981 cttccctctc tttacatcat tagcttttac tcctttagaa ggttcaagtc aagaaggaat
     2041 agatgcagac tctgaagatc atgacagggc aaaagaaatt gttaatgaaa aaggaggaaa
     2101 tgtggacgat gtacctcagc cgggcgggaa cacataatta cttaagcagt gatttaatac
     2161 ttaacaatat tttaaaaatt attgcgtaat catcagtgcc gtcttgaacg ttttctaatg
     2221 caaccaaata ttttgaattc caaattgaaa taaacttata ttttaacata aaactgcata
     2281 tgaatcatat cagcggatta atttaaaaac acattttatt acttgaataa aaacaactac
     2341 ataatacttt caggcgcaat gggtgcaggt aaaacnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnacttg aataaaaaca actacataat actttcaggc gcaatgggtg
     2521 caggtaaaac ttctgtaatc aggaagttga aagatctggg tcatgcttgt gtggatgaac
     2581 ctgccagaca gatacttgaa gagcaaagaa aaattggcgg caatggtctt cccgaaaaag
     2641 atccgaaact gtttacagaa ctgatgttat caagaactat ttttcagtat aaattacatg
     2701 agaaaaatag tgaaccggtt ttctttgaca gagccatgcc ggactttatt gcctattcaa
     2761 atttatttaa tgttgatgaa acagttttta gaaatgcagc aaaggaatac cgcttcaata
     2821 aaacattatt tttttttaga ggaacagaat ccatttatac tacagacaat gaacgaaaga
     2881 tgagttttga tcaggcagat gaattcggga ataatgttcg gaagatctat cgggagctgg
     2941 gatatgagat tcatgatgtt ccgtttctgg atattggatc gagagtggaa tttattttgg
     3001 aaaaaatttg agaacttttt ggtgattctg aaaacttgca taatttaaat acagaatatt
     3061 ttttaaccgg ttacaaattt tcagaacact aaaaagtaca gatcaataac acaactaaat
     3121 agcatacaac gttaagtttg cattttattt aaaaaaataa ttttacaata ttgagccggc
     3181 atgataataa aaatagaaaa cttaaaatat gagatatgac aacacggtat ttaaaaatta
     3241 atattccgga agatattcag gcaataaaag aagatggaat acttcttgtt atcagatcca
     3301 ttctggatac ttataaaaat aatagtaatg aatactttac tgatgacgaa attaatttga
     3361 taaataatag ctgagaacaa agcccatcac ctcatccccc tcccacttta ccatttcatc
     3421 aaaatatctc aatagttata tttataaaaa tcgaaaaatc acatcacttt taaattaatc
     3481 aaagataaaa ttaaaaatgg aaaataattc aatcgaatcg ggcaagtgcc cgtttacagg
     3541 tgcacgtaat attggaagcg gaggaaggaa taatacagac tggtggccga atcaattaaa
     3601 aataaatatt ctgcgtcagc actcttcatt atctgacccg atgggcgaag attttaatta
     3661 tgcagaggaa ttcaaaaaac tcgattatgc cggattaaaa aaagacctgc atgatctgat
     3721 gacagactct caggtttggt ggcctgcaga ctttggacat tacggacctt actttatccg
     3781 aatggcttgg cacagcgccg gaacatacag gacagccgac ggtcgaggcg gtgcaggagg
     3841 cgggcagcag agatttgctc ctttgaatag ctggccggat aatgtcaatc tcgataaagc
     3901 tagaagactg ctatggccaa tcaagcaaaa gtatgggcag aaaatatcat gggctgacct
     3961 tttcgtgctc acaggaaatg tcgctttaga atctatggga tttaaaactt ttggatttgc
     4021 aggcggacgt gaagatcaat gggaaccgga tgcagatgtt tactggggct ctgaaaaaac
     4081 atggctcggt gatgaacgat attcaggtga ccgtgatctt gaaaatcctc tggctgcagt
     4141 tcagatgggt ctgatctatg taaatcccga aggaccgaac ggaaatcctg atccaatagc
     4201 cgctgcaaaa gatatccgcg agactttcaa acggatggct atggatgata aagaaacagt
     4261 agcgctgata gccggtggtc attcatttgg aaaaactcac ggtgcaggtg aagcttcaaa
     4321 tttaggaaat gatcctgaag gctgcgacat tgaaggacag ggacttgggt ggagcagtaa
     4381 atttaacagc ggaatgggag ttgacacgat cagcagcgga ctggaagtta cctggactga
     4441 gacaccggta aaatggagta ataatttttt tgagaatctt tttaaatatg aatgggaact
     4501 tgaaaaaagt cctgcaggcg caaatcagtg ggttgcaaaa gatgcggatg cagttatacc
     4561 aaatgctttt gacccatcca aaaagcaact tccgaaaatg ctgacaactg atctttcact
     4621 gagattcgat cctgaatacg aaaagatctc cagacgattt cttgagcatc cggaagagtt
     4681 agcagatgct tttgcacgtg cttggtttaa gctgatccat cgtgacatgg gaccaaaggc
     4741 tcgttacctt ggacctgaag ttccgactga agaactgatc tggcaggatc cgattcctga
     4801 ggtagatcat attttaataa atgaaaaaga tattgaagaa ttaaaatcta tagttttaaa
     4861 ttcaggatta agtatttctg agcttgtttc aactgcatgg gcatctgctt cgactttccg
     4921 cggatcagat aaacgcggcg gtgcgaacgg tgcacgaata cgtctcgctc ctcaaagatc
     4981 atggactgtg aataatcctt cacagcttga taaagtactg gagacacttg aagctgttca
     5041 caatgaattt aactttgatc aaaaaaataa agctgaagga aagaaagttt ctcttgctga
     5101 cctgatagta cttgccggct gtgcaggaat tgaacaagct gcaaaagatg ccagtcagga
     5161 agtaacagtt ccgttttcac ccggccgcat ggatgcatca cctgagcaga cagatgcaga
     5221 agcttttgag gcacttgagc ctatcgctga cggattcaga aattatcaaa agacaaaata
     5281 caatgtatcg accgaagaat tactgctgga taaagctcaa ttgctgaatc ttaccgctcc
     5341 tgaaatgaca gtacttatcg gcggtatgag agtgctgaaa acaaactttg ataattctga
     5401 actcggagta tttacaaaaa atcctggtac acttactaat gatttttttg tgaatttact
     5461 tgacatgaat actgtttgga aagcgacttc tgaagagaaa gatttatttg aaggaagtga
     5521 cagagctacc ggtgaattga aatggaaagg tacccgttca gatcttgtat ttggatccaa
     5581 ttcagaatta aggtcacttg ctgaagttta cggaagctcg gattgcaaag agaaatttgt
     5641 taaagacttt atcgcagcat gggataaggt tatgaatcta gatcgttttt aataataatg
     5701 taaaccgccg ttaattcaac actacgaact gacggcggtt ttttatattc tgaaataata
     5761 atcacattct aaaaaaacaa tattattgga aaatggaaaa tgtttaataa acaaatcaac
     5821 ctatcgatat aatccggaaa caattgttaa caaaaaatca cttaatttct atcacagttt
     5881 taattttcaa aaaaatgagc atttattaca tcagattgtc agtgatgcaa ttgttttgaa
     5941 gctggatatt tgccggctga tgtgtatgga gatgaagttg gctaaaccaa tcaacaagtc
     6001 aaagaaaaga tggtatgtac aacatcaaat ttaaagaaga tgtataaatt ttgggcaggc
     6061 ggaacaataa aatttacctg agaagggtaa gtttattttc tttttttaaa gcagtaattt
     6121 tgacaaaaga aggtctcctt cagccaaaac aagacgaaaa actttgggtt tttgagacag
     6181 tctcctttcc gaggctgtct caaaacacta tttttaccat taagtcacaa agacacaaag
     6241 aaaaaacttt aaaaaaagaa tgctttgcgt ctttgtgtct ttgtggtatt aattaaaatt
     6301 atgttttgag acactttcga caaaagggag gagcaaaagc agtttattaa tgctttttgg
     6361 ttttggatat gccagtataa gaattaagaa tttttccgca gagatgataa attcattatt
     6421 tctgcggaat tttttttaga atttattcaa atagaattag catataatac tataaaatat
     6481 tctcctgatt caagttaagt attgcagaga ggacttgtcc gaattgtaat taatgtagtt
     6541 tgaatgtcaa tattagtcat ttaaaagtag aaagattata tgacaaatga ttctcattcg
     6601 atttggatag acatg
//
LOCUS       DAORVQ010000180         6599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7329_length_6599_cov_4.075183, whole genome shotgun sequence.
ACCESSION   DAORVQ010000180 DAORVQ010000000
VERSION     DAORVQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_6599_cov_4.075183"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLX80_11955"
     CDS             complement(<1..237)
                     /locus_tag="PLX80_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21549.1"
                     /translation="MIIVFSLSITVPQLHFLNDISVKVFIFLTVIIMGFSFSKITEKK
                     FNQPDLLELLFLLVTASFLISSFYVNFDDNFNLFS"
     gene            complement(286..1440)
                     /locus_tag="PLX80_11960"
     CDS             complement(286..1440)
                     /locus_tag="PLX80_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HQY21550.1"
                     /translation="MEEKEINTNNDIRFYIKLFLEKKKIIIINSFIGVIVSLILVLFV
                     IDPIFYSAGIVRTSNQSSGLQSLMGLSGAGSLDFGDLAGFSGGGGATKELALYTQILT
                     SRRAVEEAIIKYNIIEEEEFKYMFDAVKYFRKNIMEINQDKMAGTLEIGIYDKDPVKA
                     KDIADFMIQQLNKIHSEMSSLNAKNNKEFLESRYESVKQDLKQVEDSLTFFQNNFGIS
                     PEITVQAAIKAEVELEAEIKSEELKLELLRKIISSGEPEILTQEDKISALKNQLNIVK
                     ETPADINSVLGLKGSPEVVMNFLRLRRNVEIQNKILTTIIPILEQSKIEEKKETPTVL
                     ILDPPNVPDKKAKPKRATIVLLITFLSGFLTYLFFLIKIKWKEFKGSYNF"
     gene            complement(1487..3205)
                     /locus_tag="PLX80_11965"
     CDS             complement(1487..3205)
                     /locus_tag="PLX80_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQY21551.1"
                     /translation="MKKLILFTLLVFLINAAYTFAQFKSKEKDTEDPMSKILNQSGRG
                     NTNTGINADAPDIFDQMGSQNTFTTKSIPIEGAIDPDVYIVGPNDLFTVGLYGYINQQ
                     IPIVVSPEGTVVIPTVGAVDVAGLTLSSAKQKVVSAVKQRYYSSDVSFTLNTPRTFLI
                     KVSGLTQGNFEVSPVTRVSEILKVLMFDTLNTSRNFYEKTNEREFFNTQISLRNIKLI
                     RKNGQVINVDIYKYFLTNQDSHNPFFLEGDLLKIPYLQLDNNFINIGGAVQLGGSYEY
                     ADGDDLETIVGIARGFDVSAEPDSIVLYRAVAGSSDFKSIDLNFEKDKKFDIESFDRI
                     YVKSKINYQKNLSVLILGEVLRPGYYPISFKNTRLKDVIEMAGGLKEKAYLPLSILFR
                     DYDAEYTAKDTSEILINLRANDLIVTEMDKENYIADIQSRRNRVIVDFDRLLNNNDES
                     QNVLLEAKDIIYINDNKNIVYVFGQVGQEGYVPYKKGADVDYYIKKAGGYALAAEEDD
                     TRVIKFNTRNWYEPDEAVIESGDFVYVPKEVNQPFSFTIGLMAQIASVLLAFLTTYVL
                     IRDSNK"
     gene            complement(3202..4614)
                     /locus_tag="PLX80_11970"
     CDS             complement(3202..4614)
                     /locus_tag="PLX80_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HQY21552.1"
                     /translation="MSNISEPVKFGTDGWRAIIADTYTFENVKRVALATARVFKDHKK
                     ISTGIVIGYDTRFQGKEFAHAAATVFGNEGIKTKLTDYFVTTPTVSLLARDLGCALGV
                     MITASHNPYQYNGYKLKDEFGGSMDPDEIVKIEAELKNIKSVETPKSFEQLLNEGVIE
                     YFKGNEFYLNYLKDRINIDVIKKADLKVIYDAMYGAGQNLIGHFVDVKQIHGEVNPSF
                     GKSAPEPVQKNLSEICEYMKYNKFDLGIVTDGDADRIAIIDENGDFLDAQKTFVLLIK
                     YLFEKKKLTGKVVRGFSTSDLVRKYCEKNNIELDTVPIGFKHISKIMINDDVMIGAEE
                     SGGIGIKGHLPERDGIYNGLLYMEMLAEYGKSITELKKEFDDEFGLYFYQRHDVKTTE
                     EKKIKALQISKELQEGDEIAGQKILKKDDLDGSKFYFENGWLLVRASGTEPLIRIYCE
                     TYVEDKTSEILNKTKEFLGL"
     gene            complement(4672..5106)
                     /locus_tag="PLX80_11975"
     CDS             complement(4672..5106)
                     /locus_tag="PLX80_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQY21553.1"
                     /translation="MSKNNNQQNKIRVLIGKVGLDGHDRGAKVIAAAFRDAGIEVIYT
                     GLRQTPETIVEAALQEDVNAIGISILSGAHMTVFPKVKKLMDSKGLNDVLLFGGGIIP
                     DQDIEELRKMGVGELFTPGTPTYETIEYVKNWVSKINEGKSI"
     gene            complement(5151..6134)
                     /locus_tag="PLX80_11980"
     CDS             complement(5151..6134)
                     /locus_tag="PLX80_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQY21554.1"
                     /translation="MFKKYKKKILLSVALGALVFLGFSIYADFDKLIATFAEFNWYFF
                     PVIIGLTFLNFLIRFFKWEYYLRLLKISLKRKTSFLIFMSTFVMSVTPGKMGELLKSY
                     LLREENGTPVARSAPIILAERLTDFISVVILCITGAFVFNYGQTIVIGVGVFFVVCVM
                     LISSRKTSIALIRYLEKIKLLSKVANKFEDAYESIYTLVRVKPLIIATSMSVVAWFCE
                     CLGLYIILKVYSGITHIDVSLLSAVFIYGFSTIVGSIAMLPGGLGVTEASMTGLLVLL
                     KIPKDVSVASTFIIRVATLWFAVLVGIISVYFYQKYSHKDLENIATSQMEA"
     gene            complement(6134..>6599)
                     /locus_tag="PLX80_11985"
     CDS             complement(6134..>6599)
                     /locus_tag="PLX80_11985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQY21555.1"
                     /translation="ETSDQDPLHSVRYNSVLKSKTVAYLFKKEIVIGADTIVVLGKEI
                     INKPSDLNEAKLILKKLSGKVHEVYTGVNVINTTNGKQKFTFEKTKVHFRKIDKDEID
                     YYVKKYKPLDKAGAYGIQDDFGCLFIKKIEGDYYNIVGLPLVKLYESLRKVI"
BASE COUNT         2087 a         1260 c          877 g         2375 t
ORIGIN      
        1 agaaaacaaa ttgaagttat catcaaaatt cacatagaag ctggagatta aaaatgaagc
       61 agttactaat agaaataata attcaagcag atctggttgg ttaaattttt tttctgtgat
      121 tttggaaaat gaaaatccca taatgatcac agttaaaaag atgaatactt ttacacttat
      181 atcatttaag aaatgaagct ggggaacagt tatacttaaa gaaaaaacta ttatcaaata
      241 aattaatatt agtttttcat ttagttgaaa tttgctagac attgatcaaa aattataact
      301 acccttaaat tctttccatt tgatcttaat cagaaagaat aagtaggtta aaaaacctga
      361 aaggaaagta attaataaaa cgatagtagc tctttttggc ttagcttttt tatcgggtac
      421 attcggaggg tctaagataa gaacagttgg tgtttctttt ttttcttcaa ttttagattg
      481 ttcgagtata ggtattattg tagttaatat tttattttgt atctcaacat ttcttctaag
      541 tcttaaaaaa ttcattacta cttcaggtga tccttttaat cctaatacag agtttatatc
      601 tgcaggagtc tcttttacta tgtttaattg attttttaaa gctgaaattt tatcttcctg
      661 agttaaaatt tcaggctcgc cagaagaaat tattttcctt aaaagttcaa gcttaagttc
      721 ttcacttttt atctcagctt ctaattcaac ttctgcttta attgctgcct gaacagttat
      781 ttccggggaa ataccaaagt tattttgaaa aaaagtcaga ctgtcttcaa cttgcttaag
      841 gtcctgtttt actgattcat atcttgattc aagaaactct ttattattct ttgcatttaa
      901 agaactcatt tctgaatgta tcttattaag ctgttgaatc ataaagtcag caatatcctt
      961 agcttttaca ggatctttat cgtagatgcc aatttccaga gttcctgcca ttttatcctg
     1021 atttatttcc atgatattct ttctgaaata ctttacggcg tcaaacatat atttaaattc
     1081 ttcttcttca attatattat atttaataat tgcttcttca actgctcttc tgctggttaa
     1141 aatctgggtg tataatgcta attccttagt cgcaccacct ccacctgaaa atcctgccag
     1201 gtcaccaaaa tccagcgaac ctgcaccaga aagtcccatt aagctttgaa gtcctgaact
     1261 ttgatttgag gttcttacaa ttcctgcaga ataaaatatt ggatcgatta caaataatac
     1321 aagtataaga gatactatta caccaataaa agaatttata attattattt tctttttttc
     1381 taaaaacaat tttatgtaaa atcttatatc gttatttgta tttatttctt tttcttccat
     1441 ttcagattaa attgaaaaaa taattcttct gtatcacatt catatatcat ttattactat
     1501 ctctgattaa tacataagta gttagaaatg ctaataatac cgaagcaatc tgtgccataa
     1561 gtcctatagt aaaactgaac ggctgattaa cttcttttgg gacataaaca aaatcacctg
     1621 attcgataac cgcttcatca ggttcatacc agtttcttgt attaaattta attactcttg
     1681 tatcgtcttc ttcagctgca agggcataac cgcctgcttt tttaatataa taatctacat
     1741 cagctccttt cttataaggc acataacctt cctgacctac ctgcccgaat acataaacaa
     1801 tatttttatt atcatttata taaattatat ccttagcttc aagtaaaaca ttctgagact
     1861 catcattatt attcagtaat ctatcaaaat caacaatgac cctgttcctt cttgattgta
     1921 tgtctgcaat ataattttct ttatccattt cagttacgat cagatcattc gccctcaaat
     1981 ttatcagtat ctcggaagta tcttttgctg tatattcagc atcatagtct ctgaataaaa
     2041 tactaagcgg caaataagct ttttccttga gacctcctgc catttctatt acatctttta
     2101 gtctcgtatt cttaaatgat atcgggtaat atcccggtct taatacttct ccaagtatca
     2161 gcacagaaag atttttctga taatttatct ttgattttac atagatcctg tcaaaactct
     2221 caatatcaaa ttttttatct ttttcaaaat taagatcaat tgatttaaaa tcacttgaac
     2281 ctgcaacagc tctgtaaagt actatactat ctggttcagc gcttacatca aatcctcttg
     2341 caattcctac aatagtttca agatcatccc catcagcata ttcatatgag cctccaagct
     2401 gaactgcacc accaatatta ataaaattat tatcaagctg aagatatgga atctttaaaa
     2461 gatctccttc taaaaaaaac gggttatgtg aatcttggtt ggtcagaaaa tatttgtaaa
     2521 tatcaacatt gatgacttgt ccgttctttc ttataagttt tatatttctg agagagatct
     2581 gagtattaaa aaattccctt tcattggttt tttcataaaa attccttgaa gtgtttaatg
     2641 tatcgaacat tagaactttt aaaatttccg acactcttgt tactggagat acttcgaaat
     2701 ttccctgagt aagacctgat acttttatca aaaatgtccg gggagtatta agagtaaaac
     2761 taacatcact ggaataatat ctttgcttta cagcacttac aaccttttgc tttgcagatg
     2821 atagtgtcaa tcctgcaacg tcaacagctc cgactgtcgg aataacaaca gttccttcag
     2881 gtgaaacaac tatcggtatc tgctgattga tataaccata gagtcctact gtaaacagat
     2941 cattaggtcc gacaatataa acatccggat ctattgcacc ttcaatcgga atagactttg
     3001 tagtgaatgt attctgactg cccatctgat caaaaatatc cggtgcatct gcatttattc
     3061 ctgtatttgt atttcctctg ccagactgat tgagaatttt actcattgga tcttcagtat
     3121 ctttttcttt gcttttgaac tgagcaaaag tataagcagc attaattaaa aataccagca
     3181 gtgtaaataa aattagtttt ttcataaccc taaaaattct ttagttttat ttaagatctc
     3241 tgaagtttta tcttcaacat aagtctcaca atatattctg atcagcggct cagtacctga
     3301 agctctcaca agcagccaac cattttcaaa ataaaattta cttccgtcaa gatcatcttt
     3361 ttttaggatc ttctgccctg caatttcatc accttcctgt agctctttag aaatctgaag
     3421 agctttgatt tttttctctt cagtagtttt cacatcatgt ctctgataaa agtataatcc
     3481 aaattcatca tcaaattctt tttttaactc cgtaatactc ttaccatatt cagcaagcat
     3541 ttccatataa agaagtccgt tataaatccc gtccctttca ggaagatgtc cttttatccc
     3601 aataccaccg ctttcctctg caccgatcat aacgtcatca tttatcatga tctttgaaat
     3661 atgcttaaat ccaattggca cagtatcaag ctcaatatta tttttttcac aatattttct
     3721 aacaagatca cttgtcgaaa atcctctgac tactttaccc gtcagtttct ttttttcaaa
     3781 taaatatttt atcagaagta caaaggtttt ttgagcatca agaaaatctc cgttctcatc
     3841 tatgattgct attctgtcag catcaccgtc tgtcactatt cctaaatcaa atttattata
     3901 tttcatatac tcacagattt ctgataaatt tttctgaaca ggctcaggtg ctgattttcc
     3961 aaatgatgga tttacttcac cgtgaatttg ttttacatct acaaaatgtc ctattaaatt
     4021 ctgacctgct ccgtacattg catcatagat aacttttaaa tctgcttttt taataacatc
     4081 tatatttatt ctgtctttta gatagttcag ataaaattca tttcctttga aatactcaat
     4141 tacaccttca ttaagtaact gctcaaaact tttcggtgtt tcaacagatt ttatattctt
     4201 aagctcagct tcgatcttaa ctatctcatc aggatccatt gatcctccga attcatcctt
     4261 tagcttatat ccattatatt gataaggatt atgtgaagca gttatcatga cacctaaagc
     4321 acaaccaaga tctcttgcaa gtaaagaaac agttggggta gtaacaaaat agtcagtcag
     4381 ttttgttttt atcccttcat ttccaaagac tgtggcagct gcatgtgcaa actctttacc
     4441 ctggaatctt gtatcgtatc caattacaat tcctgttgat atttttttat gatccttaaa
     4501 aactcttgct gttgcaagag caactctttt aacattttca aatgtatagg tatcagcgat
     4561 aatcgctctc cagccgtcag taccgaattt taccggctct gaaatattac tcataattta
     4621 atgaatgtga tttaataaaa taaaaaaaat gtaaaaatct gaatattctg attatataga
     4681 tttcccttca tttatttttg aaacccagtt ttttacatat tcgatagttt cataggtagg
     4741 tgtgcccggt gtaaagagtt ctccaactcc catttttcta agctcttcaa tatcctgatc
     4801 cggaataata ccgcctccga ataacaatac atcatttaga cctttactat ccattagttt
     4861 ttttactttt ggaaacactg tcatatgtgc accgctgagg atactaattc caatcgcatt
     4921 gacatcttcc tgaagtgctg cctcgacaat tgtttccggt gtctgtcgta gacctgtgta
     4981 tataacttct attcctgcat ctcgaaaagc tgcagcaatc acttttgctc ccctgtcgtg
     5041 accgtcaaga ccaacctttc cgatcaatac gcggatctta ttttgctgat tattattttt
     5101 tgacatatag tttgtaattt aacccaaata ctgtatttct tcaagtgaaa ttatgcctcc
     5161 atctgagaag tcgcaatatt ctccaaatcc ttgtgagaat acttttgata gaaataaacg
     5221 gaaattattc caaccaaaac ggcaaaccat aatgttgcaa ctcttattat aaaagtcgat
     5281 gctactgata catcttttgg tatctttaat aaaaccagaa gtcctgtcat tgatgcttct
     5341 gtcactccaa gcccgcctgg aagcattgca attgaaccaa caattgtaga aaacccatat
     5401 ataaaaactg cagataaaag actgacatca atatgcgtga tccccgaata aactttaagg
     5461 ataatgtaaa gtcctagaca ttcacaaaac catgcaacga cgctcatcga agtcgcaatg
     5521 atcagaggtt tgacccttac taatgtgtaa atactttcat atgcatcctc gaatttatta
     5581 gcaaccttac ttaacaattt tattttttca agataacgta ttaaggctat cgatgttttt
     5641 cttgaactga taagcattac gcagacaaca aaaaatactc caactccgat cactatcgtc
     5701 tgaccataat taaaaacaaa agcacctgtt atgcagagta tcactacaga aataaaatct
     5761 gtcaatcttt ctgccagtat aatcggtgct gatctagcga caggtgttcc gttctcttct
     5821 cttaataaat atgattttaa aagttcaccc atcttgccgg gcgtcacact catcacaaaa
     5881 gttgacatga aaataaggaa actggttttt ctttttaaac tgatctttaa aagtcttaga
     5941 taatattccc atttaaaaaa tcttatcaga aaatttaaaa atgttagtcc tattattaca
     6001 ggaaaaaaat accagttaaa ttcagcaaac gtcgctatga gtttatcaaa atctgcatag
     6061 atgctgaagc ccagaaagac cagagctcca agtgctacag aaagcagtat tttcttttta
     6121 tattttttaa acatcagatc acttttctca aactttcata taattttaca agaggaagtc
     6181 ccactatatt ataataatca ccttcgatct ttttaataaa aagacatccg aaatcatcct
     6241 gtattccata tgccccggct ttatctagtg gtttgtattt tttgacataa tagtcaattt
     6301 catctttatc aatttttctg aaatgtactt ttgttttttc aaatgtaaat ttttgtttac
     6361 cgtttgttgt gttaataaca ttcacaccgg tataaacttc atgaacttta ccactgagct
     6421 tttttagaat caattttgct tcgttcagat cagaaggctt attgataatt tctttcccaa
     6481 gaactactat tgtatctgca ccaatgacta tctctttttt aaaaagataa gcgacagttt
     6541 ttgattttaa aacggaatta tatctgactg aatgcagagg atcttgatca gaagtttcc
//
LOCUS       DAORVQ010000181         6521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7480_length_6521_cov_5.690380, whole genome shotgun sequence.
ACCESSION   DAORVQ010000181 DAORVQ010000000
VERSION     DAORVQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7480_length_6521_cov_5.690380"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /gene="yihA"
                     /locus_tag="PLX80_11990"
     CDS             <1..395
                     /gene="yihA"
                     /locus_tag="PLX80_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQY21556.1"
                     /translation="INEKFYLVDLPGYGYAKVPEQIRAGWRKLVEDYISERQNVRLVF
                     VLIDSRHEPTYLDELMVSWLEYYEIPFAVILTKSDKISPNKMQKQIYRSSKIVNNEDL
                     CIDYIPFSTITGEGKNEVIKLIEDYLDA"
     gene            454..1359
                     /locus_tag="PLX80_11995"
     CDS             454..1359
                     /locus_tag="PLX80_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY21557.1"
                     /translation="MYKVLIADDVDPSGIEKLSGKKFNIVYKPGISDNEIFSEYSDFD
                     ILVIRSVRNIDKEFLNKVNFKLIATCSKGTDHIDLKYAAFKKVKVINAEEGNTISAAE
                     HTLALILNIYKGVLISDSLVRKNIFTETGYRRNELIGKTIGIIGFGKVGSHVGKLCKA
                     FGMKVVVNDIDNKLKIRYKNFNFTDLKTILKYSDIVSVHIPMSKKNHNFISESKLNLL
                     KKDCVLINTSRGGIIDEDHLLDMLRNKKIRSAGLDVFRNEPNINKGFAGLDNVLLTNH
                     IAGKTVESKQRISEFIFNKINKYKL"
     gene            1568..3313
                     /locus_tag="PLX80_12000"
     CDS             1568..3313
                     /locus_tag="PLX80_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21558.1"
                     /translation="MLQKLLTLSILVLFISVGMVQSRDDRTERTHKVTDGSEIYQNDG
                     SFQPNFVSTYYPPVHGASLTYTFQPTGVFTVYDLQSNGVPNEIWQDPLTPLNVHATFM
                     YSTVPGFATRSCAYMFSNDGGSTWTFLGDVPSSGRSGFPSISGYTNGAAVIANHNDNG
                     GTTTRTKINYDAGPGFGVFTQLDPGDSPGGQAIWPRVVGIESDKTAFASAINGQILSY
                     TNSSNNGTFSGWVEYNGDQAETYSLSVAPNGTVGHAYIGAGNGTDDNDVFYRFSTDNG
                     VTWSTSQRIWDWNIATDSLGCLRGVSMSFGTNNEPYVAFNISTLTETGFFPGLPSSIR
                     LWSPAVNGGVPVIMADQSTVPFFPNQGATTDAFLPLCRPSIGRSSVGGGLFCAYNATT
                     GEYGTDTSAYYAVWFSYSGDFGNTWSTPERVTPATPLRDWRFVSVSQVNNTSGNSTLV
                     QMLVQSDSLAGTHVNGSPIGQGEAVGIKTEVIVGINNISSTVPGDYSLAQNFPNPFNP
                     TTSIRFDIKNTSNITLKVYNANGQEVATLVNNEVVTPGTKEVSFNGNNLSSGIYFYTL
                     FAGDFKETKKMMLIK"
     gene            3451..6333
                     /locus_tag="PLX80_12005"
     CDS             3451..6333
                     /locus_tag="PLX80_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY21559.1"
                     /translation="MTNTFKITLSLLFVLCLTFSNNYALDKQGTTGKIAGKVTDAKDK
                     SPLIGATIKVDGTNLGAITDESGEFTILNVDVGEYSVTASYIGYDPVTVPGVRISADL
                     TSRVDFSLNLQGGAIVTDEIVITGKRNAISPDQSGKIIGQEFIDNSGLRGIENIASTT
                     AGVVQDERGTAINIRGGRNNETAVIVDGVLTTNPLDGTSNSYVSNNVLEEMSVLTGGF
                     SAEYGNVLSGVINVTTKGGTDKFSGTIEGITDQILSGDASQGYNVYNAALGGPLIPTK
                     ELSRAINFFAGYESDFSLVYNPSWVADQLNFGNNVIPEYTLNRWSFNGKLNVDLQEFD
                     KDLPIQLKFGTGLSSVDRRGFIQSYMLFNSVRNPLITEDNSQYYGKINHQINSKLFYE
                     LQFNYFEAEGTEGDQQFGTEWYNYGNPSAVPGLTTPGGSVGFDEYGIFAKFNRVRNYY
                     EASKISYWGLNLNLTTQVKNNEIKFGGDYKYNTLRYMDVRPNGLYNFRNSTEAQQLSA
                     FYGGTGLANTYGYGGVFNPATNQLEFGEINEGFDDAKHPIIAALYIQDKIEFKDFTLN
                     AGIRWDYLDANSWRVNDLANIAGDNGALGPEDFNEDSDPASQFSPRLGMSFPVTNNSV
                     FHAQYGTFIQLPQLEYLYNGRRNLEYWVNSAGFSGSFGNPNLGPEKTTSYEIGIKQQV
                     GDRLAVDITAYYKETEDLIGIKKFPQLPNQIQVYANQDYGTLRGLDFTIDLRRTERLS
                     LNIALGISYASGTGSDPLSASTAAWLGERQPKLTSPLDYDQRWTGVFNADYRFGRNDV
                     PKGFWGDVLSQFGLNMLYSFNSGRPYSAKSNDQDPFAATGAGASLISPINGVYGPWNN
                     SLDMKIDKTFSFLNNSLDLNAYVYIINVFDSELVNDVWDSSGQPGSTGYLNSEAGKNT
                     ILSFNNPNGAVPTTSEEYVNRYTLRSKDVANYGPPRQYRFGLKLIF"
BASE COUNT         2296 a         1034 c         1211 g         1980 t
ORIGIN      
        1 tgataaatga aaaattttat cttgtagatc ttcccggata tgggtatgca aaagttcctg
       61 agcagatcag agccggctgg agaaaattag ttgaagatta tataagtgaa agacaaaacg
      121 taagacttgt atttgtatta atagattcca gacatgagcc tacttatctt gatgaattaa
      181 tggtgagctg gctggaatat tatgaaattc cgtttgcagt aattttaacc aaatctgaca
      241 aaatatcacc aaacaaaatg cagaagcaaa tctacagatc ttcaaagatc gtaaacaatg
      301 aagatctctg cattgattat atccctttct ccactataac cggtgaagga aaaaacgaag
      361 ttataaaact tatagaagat tatcttgacg cttaaattta tacagtaaat attcatgata
      421 actttgtgaa ttaaaaatta tttaagataa aaattgtata aagtattaat agcagatgat
      481 gttgaccctt caggaataga aaagctctcc ggtaaaaaat ttaatattgt atataaacct
      541 ggaatatctg ataatgaaat attttcagag tattccgatt ttgatattct tgttataaga
      601 agtgtcagaa atattgacaa agaattcctt aataaggtta attttaaatt aattgcaacc
      661 tgctccaaag gcacagatca cattgatctg aagtatgctg catttaaaaa agttaaggtt
      721 atcaatgctg aagaaggaaa tactatatcg gctgctgagc atacacttgc acttatttta
      781 aatatatata aaggagtttt aatttctgat agtctggtca ggaaaaacat attcacggaa
      841 accggatacc gaagaaatga actaataggt aaaacaattg gaataatcgg atttggaaaa
      901 gtaggcagtc atgtaggaaa actatgtaaa gcattcggaa tgaaagtcgt tgtaaatgat
      961 attgacaata agttaaaaat tagatataag aattttaact tcacagattt aaaaacaata
     1021 ttaaaatact ctgatatagt ttctgttcac attccgatga gtaaaaaaaa tcataatttc
     1081 atttctgaaa gtaaattgaa tttattaaaa aaagattgtg tgctgattaa tacttcaaga
     1141 ggtggcataa tagatgaaga tcatttattg gatatgctga gaaataaaaa aatcagatct
     1201 gcaggattag atgttttcag gaatgaaccc aatattaata agggtttcgc aggtcttgac
     1261 aatgtacttt taacaaatca tattgcaggt aaaactgtcg aaagcaaaca aagaatatcg
     1321 gaatttatat ttaataagat caataaatat aaattataat atatgcttga ctattcactt
     1381 cataaaatat atttttgtaa aaaaaagata tcaaagaaac tttgttacga aaatctatat
     1441 tgacaagtta caacattagt agtatattta atctaaactt aacaccaacc cttaaatatt
     1501 caaagacaca gatttaataa aaattttata attacttaat attaattaaa accaaaagga
     1561 gaaaaacatg ttacagaaat tacttactct ttcaatcctt gttttgttca tttcagttgg
     1621 aatggtacaa tcaagggatg acagaaccga aaggactcat aaagttacgg acggatctga
     1681 aatttatcag aatgacggca gtttccagcc gaattttgtt tctacatatt atccaccggt
     1741 tcacggagca tcattaacat acacctttca gccgactggt gtttttacag tttatgatct
     1801 tcagtcaaac ggagtgccaa atgaaatctg gcaggatcca ttaacacctt taaacgttca
     1861 tgcaacattt atgtattcaa cagttccagg atttgctaca agatcatgtg cttacatgtt
     1921 cagtaatgac ggtggttcaa catggacatt tttaggtgac gttccttctt caggaagaag
     1981 cggtttccca tcaatttcag gttatacaaa tggtgcagca gttattgcca atcacaatga
     2041 taatggcgga acaacaacca gaacaaagat caattatgat gcaggtcctg gttttggtgt
     2101 atttacacaa ttagatccgg gtgattctcc tggtggacaa gcaatttggc caagagtggt
     2161 tggtatcgaa tctgataaaa ctgctttcgc ttcagcaata aacggtcaga tactgtctta
     2221 tacaaattct tctaataacg gtacattcag cggatgggtt gagtataacg gtgatcaggc
     2281 agaaacttat tcattatcag tagctcctaa cggaactgta ggacatgcat atatcggagc
     2341 cggtaacggt acagacgata atgatgtatt ctacagattc tcaactgata acggagttac
     2401 ttggagtact tcacaaagaa tttgggactg gaatattgca acagattcat taggttgctt
     2461 aagaggtgtg agtatgtcat tcggtactaa caacgaacca tatgtagcat tcaatatctc
     2521 taccttaact gaaacaggat ttttcccagg gttgccatct tcaatcagac tttggtcacc
     2581 tgctgtaaac ggtggagtac ctgtaataat ggctgatcag tcaacagttc cattcttccc
     2641 aaatcaggga gcaactacag atgcattctt accactttgc agaccttcta tcggaagatc
     2701 atctgttggc ggaggattat tctgcgctta taacgcaacc accggagaat acggaacaga
     2761 taccagtgca tattacgctg tttggttctc atattccggc gatttcggta atacatggtc
     2821 aacgcctgaa agagtaacac ctgctacacc attaagagac tggagatttg taagtgtatc
     2881 tcaggtaaac aatacatcag gtaactcaac actggtgcaa atgcttgttc aaagtgattc
     2941 gcttgcagga acacacgtaa acggttcacc tattggtcag ggagaagcag ttggaattaa
     3001 aacagaggtt atagttggaa tcaacaatat ttccagcaca gtaccgggag attacagttt
     3061 agcacaaaac ttcccaaatc ctttcaatcc gacaacaagc atcagatttg atattaagaa
     3121 tacatctaat atcactttga aagtatataa tgctaacgga caggaagttg caacattagt
     3181 gaataacgaa gttgtaacac cgggaactaa agaagtatca tttaacggaa ataatctgag
     3241 cagtggtatt tacttctata ccttatttgc cggagacttt aaagaaacaa agaaaatgat
     3301 gttgattaaa taagatttta ttgtttaatc ataaataaat attacaaccc tcttaaaata
     3361 caagcaattg tattttttga gggttttttt aaaaataaga taaatgaaca aaacaattat
     3421 taatttaaat tttttctaaa agggggaaaa atgactaaca catttaaaat tactctttcc
     3481 ctcctgtttg tactttgttt gactttcagt aataattatg ctttagacaa gcaaggaaca
     3541 accgggaaaa tcgccggtaa agttacagac gctaaagata aaagcccatt aatcggagca
     3601 acaattaagg tagatggtac taatctcgga gcgatcacag acgaaagcgg tgaatttaca
     3661 atcctcaatg ttgatgtcgg tgaatattca gtaactgcat catatatagg gtacgatcca
     3721 gttacagttc ccggtgttag aatatctgca gacttaactt caagagtgga tttttcatta
     3781 aatcttcagg gtggagcaat agtcacagat gaaattgtaa taacaggaaa aagaaatgca
     3841 atttcaccag atcaaagcgg taagatcatt ggtcaggaat ttattgacaa cagtggtttg
     3901 agaggaattg aaaatattgc ctctacaaca gccggtgtcg ttcaggatga aagaggaaca
     3961 gctatcaata taagaggtgg tagaaacaat gaaactgctg ttattgttga tggtgttctt
     4021 acaacaaacc ctctggacgg aacatcaaat tcttatgttt ccaataacgt acttgaagag
     4081 atgtctgttt taacaggcgg attcagtgcg gaatatggta acgtattatc tggtgttatc
     4141 aacgtaacta ccaaaggtgg aactgacaaa ttttcaggaa ccattgaagg tattaccgac
     4201 cagatattat caggtgatgc ttctcagggt tacaatgtat acaacgcagc attaggcgga
     4261 ccgttaattc ctacaaaaga attatccaga gcaattaatt tctttgcagg atatgaaagt
     4321 gatttttcgc ttgtttataa tccaagttgg gtagctgacc agttaaattt tggaaataat
     4381 gtaattccag aatatacttt aaacagatgg tcttttaatg gtaaattaaa tgtcgatctt
     4441 caggaatttg acaaagatct tccaatccag ttaaaattcg gaaccggatt atccagtgtc
     4501 gacagaagag ggtttattca gtcatatatg ttgtttaatt ctgtaagaaa tccacttatc
     4561 acagaagata acagtcagta ttacggtaag ataaatcatc agattaattc caaattattt
     4621 tatgagcttc agtttaacta ttttgaggct gaaggaacag aaggtgatca gcaatttgga
     4681 acagaatggt ataattatgg taatccttca gcagttcctg gcttaacaac accaggcggt
     4741 tcggttggat ttgatgaata tggtattttt gcaaaattta acagagtaag aaattattac
     4801 gaagcaagta agatctctta ctggggatta aatttgaatc taaccacaca ggtcaaaaac
     4861 aatgaaatta aatttggtgg tgattacaaa tacaatactt tgagatacat ggacgttcgt
     4921 ccaaatggtt tatacaattt cagaaattcc acagaagctc agcagttatc agctttttat
     4981 ggaggaaccg gacttgctaa tacttatgga tacggcggag tatttaatcc tgccaccaac
     5041 cagttagaat ttggtgaaat caatgaaggt tttgatgatg caaaacatcc tatcattgca
     5101 gctctatata ttcaggataa aatagaattt aaggatttta cattgaatgc aggtataaga
     5161 tgggattatc ttgatgcaaa ttcatggaga gtaaatgacc tggcaaatat tgcaggtgat
     5221 aacggtgcat taggacctga agatttcaac gaagacagtg atccggcatc tcaattcagt
     5281 ccaagactgg gaatgtcatt tccagttaca aataattcag tatttcatgc tcaatatggt
     5341 acatttattc agcttccaca gttagaatat ctatataatg gcagaagaaa tttggaatac
     5401 tgggtaaaca gtgcaggatt ctccggttct tttggaaatc caaatcttgg acctgaaaag
     5461 acaacttctt atgaaattgg tataaagcaa caagtcggtg atagattagc tgttgatatc
     5521 actgcttatt ataaagaaac tgaagatctt atcggtatta aaaaatttcc tcagcttcca
     5581 aatcagatcc aggtttatgc aaatcaggat tatggaactt tgagaggtct tgattttact
     5641 attgatttga gaagaactga aagactatca ttaaatattg cattaggaat ttcttatgca
     5701 tcaggtacag gatctgatcc tctatctgct tctacagctg catggttagg tgaaagacaa
     5761 ccaaaactca catctccgct tgattatgat caaagatgga caggagtatt taatgctgac
     5821 tacagattcg gaagaaatga tgtaccaaag ggattctggg gtgatgtatt atcacaattt
     5881 ggtttaaaca tgttatattc atttaacagc ggaagacctt attctgcaaa atcaaatgat
     5941 caagatccgt ttgctgcaac cggagccgga gcatcactta tttcaccaat caatggcgta
     6001 tatggtccat ggaataatag tttagacatg aagatagata aaacattttc attcctgaat
     6061 aattcactgg atttaaatgc atatgtttac ataatcaatg tatttgattc tgaattagta
     6121 aacgatgtat gggacagcag tggtcagcct ggatctacag gatatttgaa ttcagaagca
     6181 ggtaaaaata caattctttc gttcaataat ccaaacggag ctgtaccaac aacttctgaa
     6241 gaatatgtta acagatatac tctaagatca aaggatgttg caaattacgg acctccaaga
     6301 caatacagat ttggactaaa attaattttc taaacaaaaa ttttatcggg tatcaggtta
     6361 tcctgatacc ctttattaaa tgctttttta ttgtttttgt ttaataaaat ttttaaaaat
     6421 ataaatagaa actacaatga ttaaaaaaat attaattttc ctaataccga tcgtccttgt
     6481 gtttgcctac ggttttaaac ctaaggataa accgaccagg a
//
LOCUS       DAORVQ010000182         6516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7492_length_6516_cov_4.239591, whole genome shotgun sequence.
ACCESSION   DAORVQ010000182 DAORVQ010000000
VERSION     DAORVQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_6516_cov_4.239591"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLX80_12010"
     CDS             complement(<1..415)
                     /locus_tag="PLX80_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQY21560.1"
                     /translation="MRIIFMGTPEFAVPSLSAIFKSEHDLISVVTIPDRKKGRGQNVS
                     FSEVKKFAMAKRIDFLQPDDISNEDFLEKLISLKPDLIVVVAFKILPEIIFDIPTFGI
                     FNLHASLLPKYRGAAPINRAIMNGEPETGVTTFFLE"
     gene            complement(437..988)
                     /gene="def"
                     /locus_tag="PLX80_12015"
     CDS             complement(437..988)
                     /gene="def"
                     /locus_tag="PLX80_12015"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQY21561.1"
                     /translation="MPRTLPITTLGMEILRKKTTPVKKIDAEFIKLIQDMFYTMDKAT
                     GVGLAAPQINVNKSVAVVDISYLDDYKDFKPMTLINPEIISGSGSTVEEEGCLSIPNV
                     KAEVKRFEKIILKYMDYDLNEIQTEMKGFPARVVQHEIDHLNGILFIDHLSKEKLKEL
                     KKDLTLVRKGKMDIDYDILVNGK"
     gene            1134..2045
                     /locus_tag="PLX80_12020"
     CDS             1134..2045
                     /locus_tag="PLX80_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY21562.1"
                     /translation="MVNEILISLNEFILQVLKMLPKIVLAIIVFLFFVLTNNKIIKFL
                     LKKINPIANDKVVYDIISRLFKWIYIICGFVLALNILGLGGFAGGIAAGAGLSAVVIG
                     FAFKNIGENFISGIILAFQKPFNVGDFIETSDYSGNITTVGLRTTNIKTTDGNDIFIP
                     NSMILNNPLINYSMDTIRRFDFTLQLDFGINVENVRKILLEEFQSNEDILKEPVSFIM
                     VDMLTSNIVVKCYYWLNVKTIKKPLPQTKSSIIEDTLSVLIKNGIDVSDISQIKLTND
                     FIKLGTEQIDSKNDNDNKNENIIENLK"
     gene            2082..4190
                     /gene="fusA"
                     /locus_tag="PLX80_12025"
     CDS             2082..4190
                     /gene="fusA"
                     /locus_tag="PLX80_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQY21563.1"
                     /translation="MSVKSENIRNIAITGHGGCGKTTFTEGILYASGVINRFGKVEDG
                     NTVSDYHKDEIEKMMSINSSLTNTKWKNSNGEDIKINIIDTPGFMDFVGEVRAALYVA
                     DTAVIMVDAFKGIEVGTESSFMMTNEFENNVIFLINKLDTENANYKLTVENIKDSFGS
                     RVAVVQFPINEGTGFDSVVDVIRMKVLRFNNDGKGEYTEEDIPEALKEKANSYHQKLV
                     ECIAEEDEELLNKFFEEGANLTDEEIVYGLLKGMRERKLFPVFCMSASKNIGTRDILD
                     FISNFTESPMDKPAEIGRRPNSDDPIEIPPKVEGEPTIFIFKTVSEKNIGELSFFRVY
                     SGKVAPGLDMINEANGKSERLSQLYAMNGKDRQEMGEVVCGDIAGVVKLKDSHTNNTL
                     SSKNYPVVLPEINFPKPNMTLAIEAKNKGDEDKIATSLHSLHEEDPTFIVQYNSETAQ
                     TLISGQGEIHLNTVLNRMKGKYNLEVEVSDPKIPYRETIRSTISDSEFKHKKQSGGRG
                     QFGHVHLKIEPQERGAGFEFVNAIVGGVVPGRFVPAVEKGLIEILNNGVLIGCKVIDV
                     KVTLTDGSYHNVDSDEISFKIAAAQAFKKGFKEANPVILEPIYDVEVVFPEEFMGAVS
                     GDVSSRRGRIHGMDVFGKLQKITCQMPLSEMNDYSTKLRSLTQGRGYYDRKFSHYEDV
                     PKDVETKIIGDYEKKRAEVA"
     gene            4341..5066
                     /locus_tag="PLX80_12030"
     CDS             4341..5066
                     /locus_tag="PLX80_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQY21564.1"
                     /translation="MLELFSQPDTYISLLTLTFLEIVLGIDNIIFISIITGRLPEESQ
                     GKTRTIGLILALIFRVGLLLTISWIAGLVYPLITIFNFEFSGRDLILIAGGLFLLVKS
                     TLEIHNKIEGEEEGIKSKVKENFWNIIVQIILIDLVFSLDSVITAVGLVNEISIMVIA
                     IVISMIIMIIFAKSVSDFIHKHPTIKMLALSFLLMIGLLLLIEGFHVEVPKGYVYFAM
                     AFSFIVEMLNIKMREKGKKKKVE"
     gene            complement(5243..5959)
                     /locus_tag="PLX80_12035"
     CDS             complement(5243..5959)
                     /locus_tag="PLX80_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21565.1"
                     /translation="MNKFEKIILLGRPASGKSEVINYIKNMNAEDRLNRFHLGNIKEL
                     DDFPMLWTWFEEDAILAKKFGKPRIHTDDEGYFIEQYLWNLLIERISMDYSKLIRDDK
                     DFHDHSTALVEFSRGTEHGGYKDAFNHLSKEILEKAALVYINVPYEESLRKNRKRFNP
                     NKPDSILEHGLPDDKLEKMYKLVDWEEVSAESPTHININGVSVPYAVFENMPSKTDDP
                     EVLGPELDEVFSRLWELYKS"
     gene            6256..>6516
                     /locus_tag="PLX80_12040"
     CDS             6256..>6516
                     /locus_tag="PLX80_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21566.1"
                     /translation="MQEVRNNFRHKFPIAFYFTKYNSKTTSQHFYKYSKEKTVICFDL
                     EDSVNCWINKENNTNLKNEKRKILTELLSGWEDKVVETKTAVR"
BASE COUNT         2212 a          995 c         1143 g         2166 t
ORIGIN      
        1 tctcaaggaa aaaagtagtt acgcctgttt caggttcacc gttcataata gctctgttta
       61 ttggagcagc tcctctgtat ttcggcagca gcgaagcatg cagattaaat attccaaaag
      121 tgggtatatc aaaaataatt tccggaagta ttttaaatgc aactactaca atcaggtcag
      181 gcttaaggga aattaacttt tctaaaaaat cttcattaga aatatcatcc ggctgcagaa
      241 agtcaatcct ctttgccatt gcgaattttt tcacttcaga aaaacttaca ttctgtcctc
      301 tgcctttttt tctgtcagga attgtcacta cacttatcag atcatgttcg cttttaaaaa
      361 ttgcagacaa agaaggaact gcaaactcag gcgtacccat aaatattatc cgcatatttt
      421 ttttattaat taaaaattat ttaccattta ctaaaatatc ataatcaata tccatctttc
      481 cttttctgac aagtgtgaga tcctttttaa gttcttttag cttttccttt gaaagatgat
      541 cgataaaaag tatgccgtta agatgatcga tctcatgctg tactacgcgg gcaggaaatc
      601 ctttcatttc cgtctggatc tcattcagat catagtccat atatttgaga atgatcttct
      661 cgaatctttt gacttcggct tttacattgg gaatgctcag acagccttct tcctcgacag
      721 tagatcctga tccggagatt atttcaggat ttattaatgt cataggttta aaatctttat
      781 aatcatctaa ataagaaata tcaactacag caacagattt gttaacattg atctgaggtg
      841 ccgcaagtcc aactccagtg gctttatcca ttgtataaaa catgtcctga atcagcttaa
      901 taaattcagc gtcgatcttt tttacaggtg tggttttttt tcttaaaatt tccataccta
      961 atgttgtgat aggaagtgtc ctcggcaaag tatttacttt aaattattta ttgtgcaaat
     1021 ataataaaag cgtgtatctg tataaagtaa ctttaaactt attattctgt gtattatttt
     1081 aaaattctgt ttattcgaat tattttacac aataaaaaaa gacttagatt aatatggtaa
     1141 atgaaatact gatatcttta aatgaattta ttcttcaggt actaaaaatg cttcccaaga
     1201 ttgtattggc aataattgtc ttcttatttt ttgtgctgac aaataataaa ataataaaat
     1261 ttctgctgaa aaaaattaat ccgatagcaa atgataaagt cgtttatgat attatttcca
     1321 gattatttaa atggatttat attatatgcg gatttgtact ggcacttaat atacttggtc
     1381 ttggaggctt tgctggcggt attgctgccg gtgcaggtct ctctgctgta gtgatcggat
     1441 ttgcatttaa aaatataggc gagaatttta tttcaggaat aattctggca ttccaaaaac
     1501 cctttaatgt tggtgacttt attgagactt cagattattc cggtaatatt acaactgtgg
     1561 gattaagaac caccaatata aaaactactg atggtaatga tatatttatt cctaattcaa
     1621 tgattctaaa taatccactg ataaattatt caatggatac catcaggaga tttgatttta
     1681 cgctgcagct tgattttgga attaatgtag aaaatgtcag aaaaattcta ttggaagaat
     1741 ttcagtcaaa cgaagatatt ctgaaagagc ctgtatcatt tataatggta gacatgctga
     1801 cttcaaatat agtcgtaaaa tgttactact ggcttaatgt taaaacaata aaaaaacctt
     1861 tacctcaaac aaaaagcagt attattgaag atactctgag tgtccttata aaaaatggta
     1921 ttgacgtttc agatatcagt cagataaaac taacaaatga ttttattaaa cttggaactg
     1981 aacaaattga tagtaaaaat gataatgata ataaaaacga aaacataatt gaaaatttaa
     2041 aataatttat taaataactt aaataaggag atataaaacg tatgtccgta aaatctgaaa
     2101 acattagaaa cattgcgatc accggtcacg gtggttgcgg taaaaccact tttacagaag
     2161 gtattcttta tgcctcagga gtcattaaca gattcggaaa agtagaggat gggaatactg
     2221 taagtgatta tcacaaagat gaaattgaaa aaatgatgtc gatcaattca tctctgacta
     2281 atactaaatg gaaaaattca aatggtgaag atattaagat caatataatt gatactccag
     2341 gatttatgga ttttgtaggt gaagtgagag ctgcattata cgttgctgat actgctgtta
     2401 ttatggtgga tgcttttaag ggaattgaag ttggtacaga atcatcattt atgatgacta
     2461 atgaatttga gaacaatgtt atattcctta ttaataaact tgataccgaa aatgcaaatt
     2521 ataaactgac tgtagaaaat ataaaagata gttttggatc cagagttgct gtagttcagt
     2581 ttcctataaa tgaaggtaca ggtttcgatt ctgtagtaga tgtaataaga atgaaagtat
     2641 tgagatttaa taatgacggt aaaggtgaat atacagaaga agatattcct gaagcattaa
     2701 aagaaaaagc taacagctat catcagaaat tagtcgaatg tattgcagaa gaggatgaag
     2761 aattattaaa taagtttttt gaggaaggtg caaatcttac tgatgaggaa attgtttacg
     2821 gattattaaa aggtatgaga gaaagaaaat tatttcctgt cttttgcatg agcgcttcta
     2881 aaaacattgg aacaagagat attcttgatt ttatttcaaa ctttactgaa tctccaatgg
     2941 acaaacctgc ggaaataggt agaagaccaa acagtgatga tccgattgaa ataccaccaa
     3001 aggttgaagg tgaaccaact atttttattt ttaaaactgt atcagaaaag aatattggtg
     3061 aactgtcttt cttcagagtt tattcaggta aagtggctcc gggtttggat atgataaatg
     3121 aggcgaacgg aaaaagtgaa agactttctc agctttatgc aatgaatgga aaagacagac
     3181 aggaaatggg tgaagtagta tgcggagata ttgcaggtgt tgtaaagctg aaagattcgc
     3241 atactaataa tacacttagt tcaaaaaatt atcctgtagt actccctgaa ataaatttcc
     3301 caaaacctaa tatgactctg gctatcgaag ctaagaataa aggtgatgaa gataaaattg
     3361 caacttcttt acattctctt catgaggaag atcctacttt catagttcag tataattcgg
     3421 aaacagcaca gactttgata agtggtcagg gtgaaattca tttaaatact gttttgaaca
     3481 gaatgaaagg taaatataat ctcgaagttg aagtttcaga tccgaaaatt ccatacagag
     3541 agactatacg atccactatt tctgattctg aatttaaaca taaaaagcag tcaggcggca
     3601 ggggacagtt tggtcatgtg catcttaaga tcgagccaca ggaaagaggt gcgggatttg
     3661 aatttgtaaa tgcaattgtc ggcggtgttg tgcccggcag gtttgttccc gctgtagaaa
     3721 aaggtctgat cgaaatttta aataacggag ttctcatagg ctgtaaagta atcgatgtga
     3781 aagtaacttt aacagacggc tcttatcata atgtggactc agatgaaatt tcatttaaga
     3841 tcgctgcagc tcaggctttt aaaaaaggat ttaaagaagc taaccctgta attcttgagc
     3901 caatctatga tgttgaagtt gtattccctg aagaatttat gggagctgta tctggagatg
     3961 tttcatcgag aagaggcagg atccatggaa tggatgtctt tgggaaactt caaaagatca
     4021 cttgtcagat gcctttatct gagatgaatg attattctac aaagctcaga tcacttacac
     4081 agggcagagg ttattacgac agaaagtttt cgcattatga agatgtacca aaagatgtgg
     4141 aaacgaaaat aattggtgat tatgagaaaa aacgagctga agttgcttaa ataaactgta
     4201 gttaaatcgg tctaagtttc ttttattgaa taacttatag aatatttact aaagtatttt
     4261 atgtattaat tcacagtatt acttcaggtt taaacaactg aagatttatt acaatatttt
     4321 ttaattttaa tttaatcaaa gtgttagaat tattttcaca acctgataca tatataagtc
     4381 tacttactct tactttccta gagattgtac ttggtataga taatataata tttatttcca
     4441 ttatcaccgg caggctccct gaagagagtc agggaaagac cagaacaatt ggtcttatac
     4501 ttgctttgat cttcagggtc ggattactgc ttacaatttc ctggattgct ggactggtct
     4561 atcctcttat aactatattt aattttgaat tttccggcag agatctgata ttaattgcag
     4621 gaggattatt tcttctggta aaaagtacac ttgaaattca taataagata gaaggtgagg
     4681 aagagggaat taaatcaaaa gtaaaagaga atttctggaa tataatcgtt cagataattc
     4741 ttattgatct tgtattctca ttagattcgg ttattactgc tgttggactg gtaaatgaaa
     4801 tatcaatcat ggtaattgcc attgtcattt caatgatcat tatgataatc tttgcaaaat
     4861 cggtcagtga ttttattcac aagcatccga ctattaaaat gcttgctctt tcatttttac
     4921 ttatgatcgg acttctgctg ctgatagaag ggttccatgt cgaagtacct aaaggatatg
     4981 tctatttcgc aatggcattc tcatttatag tggaaatgct gaatatcaaa atgagagaaa
     5041 aaggcaaaaa gaaaaaagtc gaataataat cattaagaaa ttctaaaaaa acctcagcag
     5101 aaattttcta ctgaggtttt ttttatttcc cgctcgttcc caaactcctg tttgggaacg
     5161 cacttgaaac tgtatctccg tttcaatcac acaaacggta aaatatcatc ttcaggattt
     5221 agttatttaa aaaaaatatt gatcaacttt tatacaattc ccacaatctt gaaaacactt
     5281 catcaagctc aggtccgagt acttccggat catctgtctt tgaaggcata ttctcaaaca
     5341 ctgcataagg aacgctgact ccgttaatat ttatgtgagt tggggattcg gctgatactt
     5401 cttcccagtc aactaattta tacattttct caagcttatc atccggaagc ccatgctcca
     5461 gaatgctgtc cggtttattt ggattaaatc tctttctgtt ttttcttaat gactcttcgt
     5521 aaggaacatt aatataaacg agtgcagctt tctcaagtat ttcttttgat aaatgattaa
     5581 aggcatcttt atacccgccg tgctcagtgc ctctggaaaa ttccacaagt gcagtactgt
     5641 gatcatgaaa atccttatca tctctgatga gttttgaata gtccattgag atcctctcaa
     5701 ttaaaagatt ccagagatat tgctctatga aataaccttc atcatcagta tgaattcttg
     5761 gcttaccaaa tttctttgca agtattgcat cttcctcaaa ccatgtccat agcataggaa
     5821 agtcatcgag ctcttttata tttccaagat gaaacctatt gagtcggtct tcagcattca
     5881 tgtttttgat ataattgatc acctcagatt ttcccgatgc aggtctgccc agcagtatta
     5941 tcttttcaaa tttattcatg taaaatattt tgattattaa aattagtcag ataaaataat
     6001 taaatataaa atgtttctct acttacttta tttacttaat tgggtattag ctttcaaaat
     6061 ttgaattgtc tgtttgactc caagacttcg ggctttaaat ttttattatt taagaagcat
     6121 cctatcgagc ttactccgaa gagtacgaaa agcacgtgac tctgcggaaa tcacagtaag
     6181 agaagtattg tccgagtgtt tttatttaaa aagtctttga ttatatttga tgtaataatt
     6241 tataaggagc atcaattgca agaagtacga aataatttcc gccataaatt tccaatagct
     6301 ttttatttta cgaagtataa ttctaaaact acatctcaac atttttataa atattcaaaa
     6361 gaaaaaaccg taatttgctt tgatcttgaa gacagcgtca attgctggat caataaggaa
     6421 aacaatacaa atttaaaaaa tgaaaaaaga aaaattctta ctgaattgct ttccgggtgg
     6481 gaagataaag ttgtagaaac aaaaaccgct gttagg
//
LOCUS       DAORVQ010000183         6498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7532_length_6498_cov_5.299239, whole genome shotgun sequence.
ACCESSION   DAORVQ010000183 DAORVQ010000000
VERSION     DAORVQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7532_length_6498_cov_5.299239"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLX80_12045"
     CDS             complement(<1..446)
                     /locus_tag="PLX80_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21567.1"
                     /translation="MKKIFFLFLMQFAFTGILLSQNYNWITPNKTYLKMYVADDGMYR
                     IDRNDFTNAGINTTGLDPRTVKLYNKGVQIPVSFNGEQDGTFDPSDYLDFYGQRNYGG
                     NVKSYDHNNNLFFTTDEYYNEYSDTNVYWLDWGGAIGTRYAQFNYPV"
     gene            complement(492..5396)
                     /locus_tag="PLX80_12050"
     CDS             complement(492..5396)
                     /locus_tag="PLX80_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQY21568.1"
                     /translation="MNIKTAMYRKLLSPVGCIFLVMLFISIFSINKNLFSQTNPNWIT
                     PNKTYLKMYVVNKGMSRIVRTDFANAGINTTGLDPRTVKVYNKGLQIPIYFSGEQDGV
                     FNDNDFFDFFGDRNYGGLVKTYDSNNNVLYVTDEYYNQYSDTNVYWVDWGGANGLRFQ
                     NLNYSVSTLYPNNYFTDKIHFEKDKLYYQGENTAATDYRFLSTEKVLGEGWYWELMYN
                     TQVLTDTFSAPVQSSSPQTSTVKLFAIPKTRNSSIINEHSLEVRINGTLISTLLSNDF
                     NKIDTTVSFSTSLLSGSSVNTVSVKYSHQPGFSGEMYFDNFEISYPRKFSLTDKKLSA
                     QINGADSTSKVFTVSGYNNSSPVYIYDILNNIRLMNFTSNADTLKFSAKGISSIQISN
                     DTIRNKPVKIKQRQVKDLVSNTNGADYLVIYNSIFESQAEQLRAYRNSHDGYRSFKAD
                     VEDIYDVFGYGIEDPLAVRYFTNYVNVNWQLPKLRFICLFGRGSLDPKKNSTGSVYYK
                     NLVPVYGNPNADGYFGNFNFGTFFYYPQVAIGRLPAYSAAEAQAMVDKIIAYESQPFE
                     NWNKNFTFITNGSTPSEQNSHQVKSNFECAVYVTSPPVSGYCTKIYRNDTSGGITYNY
                     ADSIVKDFDEGTIFVNYRGHAGSHDWEVMMQDPNVLNNGSKLPLVISLTCFTGETAKP
                     DFRAFLEKFIYLPNKGAIGTIGTTGWSFGTNGNDFGSYLLQTFKIDTTRIIGDMVKFA
                     GKSMSRDSLSFSIRHTINGYTLTGDPAVTLKYPKTADFKITQADYKLEPKTVTLSTPA
                     SLTIKPYNLGMHADSVKIRFQLVKNNFNYSFKDTVIKAFTIDREITHNFTIDTIGIYS
                     MNVILDVDNRFPLENPNDNTIQINIPLLESSFIPIRPADNSVIFKDSVQFVILNPNFD
                     YGQSSVNIYAEMDTSKYFNSPVKKTFTNLNIKGGSTIFNTSVPVLNNNTLYYWRSRSI
                     INSDTSGWSQSNNFIYNNGIGFDSPKMRPNQENLPVVISKSNKDQFDISELNNTEYSD
                     GGIRLNEEDAVLYVRSYGSNAEEASYFSVGNKNIFIDGGKNTGLNFLKVNKLTGKILE
                     FKNLKMSSAASSDSVITFLNTFDTAQYMMLLSAAYFPGSQTFSAGARSKLREFGSVYA
                     DTIGLLGYFHSWAFIGYLGANSSQVTEMFDPCCQPVLTCLDCSHWFEAVVSMNVKFKK
                     SSGTVTNIIGPASTWTDFSWKADIYPNSSIGFDVIGIDRNGAETMLRPNVQTSEFNEL
                     GSVSAEQYPKLKFNALLDMDTLSGFQSPVLRSLKVNYSAASELVFVKNSLQFSTSDKD
                     KNAVNFSFDYANGGYKYLFGTVVNLYSKSISGSNLISSDTVTQLLKTDSTLTYSNSFT
                     MPNFRDSSRVWIEINPKDGQNEFYTFNNSGSIPLTSTAQSVRNEVKIIADGKLLQDGE
                     SVRKDPEIRIELTGERNTGIMQDTSMLIINLNGYKIPYYLKGNLNPALKTIEGDNQTG
                     SGSIAVLLKTVLNSGSNRLGIRYLNDNGNTDSLYMDLIVSDESGITGVYNFPNPMKNE
                     TAFIFDLNTPDIIPGVRIKIYTVQGRLIRELNVQPNIGVNQIIWDGRDEDGDNIANGT
                     YLYKIFTDGNIIAETSVQKLVMLR"
     gene            complement(5421..6389)
                     /locus_tag="PLX80_12055"
     CDS             complement(5421..6389)
                     /locus_tag="PLX80_12055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21569.1"
                     /translation="MDFNTNSGKLVSVIIPFFNRVDLLKSSIKSVINQTYKPIEVILV
                     DDFSSEKFDLKEIEKYNSSDFNIKLIRNSKNLGPGLSRETGRLVAKGDFFAYLDSDDF
                     WHKEFLEKLVNSMDKNPAAGMAYSKTLLIRSKGNFLRNKNDKTYDSIIPIIFDMHGRP
                     WATGACVWRREIVNKIGPWTKSRIWEDYEYDIRAAKVNNNIIHVPEILFYVNMDSKEK
                     ISLVRNTKKMIEDKARSILNISRQLRNSKFSKEKEIQERITYFLLTSCATLSDYKSEM
                     QIINEMFNEFYNWKNRKNYIVNAMTYVSPARVNSKIFRIIRQQSRN"
BASE COUNT         2031 a         1162 c         1053 g         2252 t
ORIGIN      
        1 acgggataat taaactgagc atatcttgtc ccaattgctc caccccaatc taaccagtac
       61 acattggtat cagaatattc attgtaatat tcatcggttg taaaaaataa attattgtta
      121 tgatcataag actttacatt tccgccataa ttcctttgtc cataaaaatc taaatagtca
      181 gaggggtcaa atgtaccgtc ctgctcaccg ttaaaactga caggaatctg aactccttta
      241 ttgtaaagct ttacagtacg aggatctaaa cctgttgtat taattcccgc attagtaaaa
      301 tcattcctgt cgattcggta catgccgtca tctgcaacat acatcttcag gtatgttttg
      361 tttggagtga tccagttata gttctgagaa agcaaaatgc ccgtaaatgc aaattgcatt
      421 aaaaaaagga aaaatatttt tttcatataa ataaatttaa aactaattga tttttatttt
      481 tgagaaatta attaacgaag cataacgagt ttttgaacag aagtctcagc aataatattc
      541 ccgtctgtaa atattttata taagtaagtt ccgtttgcaa tattgtctcc gtcttcatct
      601 ctgccatccc aaatgatctg attgacacct atattcggtt gaacatttaa ctccctgatc
      661 agtcttccct gaacagtata aattttgatc ctgacgcccg gaataatatc aggtgtattc
      721 aaatcaaaaa taaatgccgt ctcatttttc ataggattag gaaaattata aactcctgta
      781 attcctgatt catctgacac aatcaggtcc atatataatg aatcagtgtt tccgttgtca
      841 ttcagatatc tgatgccgag ccggtttgat ccggaattaa gcacagtttt taaaagtact
      901 gcaatgcttc cggaaccggt ttgattatcg ccttcaattg tcttcaatgc cggattcaaa
      961 tttcctttga gataatatgg aattttatag ccgttaagat ttatgatcag cattgatgta
     1021 tcctgcatta tacctgtatt tctttcacct gtaagttcaa tccggatctc aggatctttt
     1081 cttactgatt caccgtcttg aagcagttta ccgtcagcaa ttatttttac ttcattcctg
     1141 acagattgag cagtacttgt caatggaata cttccgctgt tattgaaagt ataaaattca
     1201 ttctgtccgt cttttggatt tatttcgatc catactctcg agctgtccct gaaattcggc
     1261 attgtaaagc tgttagaata agtgagagtg ctgtctgttt ttaataactg agttacagta
     1321 tcagatgaga tcagattcga acctgatatt gatttggaat ataaatttac aactgttcca
     1381 aataaatatt tatatccgcc gttagcataa tcaaaagaaa aattgactgc atttttatct
     1441 ttatcagaag tactgaactg taaagaattt tttacaaata ctaattcaga tgctgctgaa
     1501 taatttacct tcagagatct caggacaggg gattgaaatc ctgaaagtgt atccatatcg
     1561 agtaatgcat taaacttaag tttaggatac tgctctgcgc tgaccgatcc gagttcgtta
     1621 aattctgaag tctgcacatt tggtctgagc attgtttccg caccgtttct gtcaattcct
     1681 atgacatcaa atccgatcga actgtttgga taaatatctg ctttccatga aaaatcagtc
     1741 cacgtgcttg ccggtccgat aatattagtt acagtaccgg atgatttttt aaatttcaca
     1801 ttcatgctta ctactgcttc aaaccagtgc gagcagtcca gacatgttaa taccggttga
     1861 caacatggat caaacatttc agttacctgt gatgaatttg ctccgagata tcctataaat
     1921 gcccatgaat gaaaatatcc gagcagtcca attgtatctg cataaacact tccaaattct
     1981 ctgagtttag atcttgcacc tgcagaaaaa gtttgtgatc cggggaaata agctgcactt
     2041 agaagcatca tatactgagc tgtgtcaaat gtattcagaa atgttattac tgaatcagaa
     2101 gaagctgcgc tgctcatttt cagatttttg aattccagaa tcttgccggt gagtttattt
     2161 actttgagaa aattcaaacc agtatttttt ccaccgtcga tgaatatgtt tttgttgcct
     2221 acactgaaat aagatgcttc ctcagcatta cttccgtagg atctgacata aagtactgca
     2281 tcttcttcat tgagtctgat ccctccgtca gaatattctg tgttatttaa ttcagaaata
     2341 tcaaactgat ctttatttga tttagaaatt actacaggca gattttcctg atttggacgc
     2401 atcttaggag aatcaaatcc gattccatta ttataaataa aattattact ctgtgaccag
     2461 ccagaggtat ctgagtttat gatcgatctt gatctccagt aatacagagt gttattattc
     2521 aatacaggaa cggaagtatt gaaaattgta gaaccacctt ttatgttcag atttgtaaaa
     2581 gtttttttaa caggagaatt gaaatatttt gaagtatcca tttcagcata aatatttaca
     2641 ctgctctgac cataatcaaa attaggattt aggatcacga actgcacgct gtctttaaat
     2701 attactgagt tatcagcagg tctgatcggt atgaaagaac tttcaagtaa cggaatatta
     2761 atctgtatag tattatcatt gggattttca agtgggaatc gattatcaac atcaagaatt
     2821 acattcatac tgtaaatacc aattgtatca attgtaaagt tgtgagtaat ttctctatct
     2881 attgtaaatg cttttattac agtatcttta aaggaataat taaaattatt ttttacaagc
     2941 tgaaatctta tctttactga atcagcatgc attccgagat tataaggttt tatagtaaga
     3001 gaagcaggag tacttaaagt aactgttttt ggttctaatt tataatctgc ctgagtgatc
     3061 ttaaagtcgg cagttttagg atatttcaaa gtaacagcag ggtcgcctgt cagagtataa
     3121 ccattaatag tatgtcttat agaaaatgat aatgaatctc tgctcatact tttacctgca
     3181 aattttacca tgtctcctat tattcttgta gtatctattt taaaagtctg aagcaggtaa
     3241 gacccaaagt cattgccatt tgtaccgaaa ctccagccgg tagtaccgat cgttccgatt
     3301 gcacctttgt ttggcagata tataaacttt tctaaaaatg ccctgaaatc tggctttgca
     3361 gtttcacctg taaaacaagt aagactaata accagtggta atttacttcc gttatttaaa
     3421 acattcggat cctgcatcat cacttcccag tcatgacttc cggcgtgacc tctgtaattt
     3481 acaaatattg ttccttcatc aaaatctttc actatagaat ctgcataatt atatgtaatg
     3541 ccgcctgaag tatcatttct gtagatcttc gtgcagtatc ctgaaaccgg tggagaagta
     3601 acataaacag cacattcaaa attagatttt acctgatgag aattttgttc agaaggcgtg
     3661 ctgccattgg ttataaaagt aaaattttta ttccagtttt caaaaggctg tgactcatat
     3721 gctatgatct tatccaccat tgcctgtgct tctgctgctg aataagcagg gagtctgcca
     3781 attgcgacct gcggataata aaagaatgta ccaaaattaa aattgccaaa ataaccgtca
     3841 gcatttggat taccatacac cggtacaaga tttttataat aaaccgagcc ggtagaattt
     3901 tttttcggat caagtgagcc tcttccaaaa agacaaataa atctaagctt tggaagctgc
     3961 cagtttacat ttacataatt agtaaaatat cttacagcca gaggatcttc tatgccgtat
     4021 ccaaacacat cataaatgtc ttccacatca gctttaaatg atctatatcc gtcatgagaa
     4081 tttctgtatg cccgaagctg ttctgcctgt gactcaaaga tcgaattata aataacaaga
     4141 tagtcagctc cgtttgtatt ggacacaaga tccttaacct gacgctgttt gatctttaca
     4201 ggcttatttc tgatagtatc atttgagatc tgaatgctgc tgattccttt tgcagaaaat
     4261 ttcagagtat cagcgtttga agtaaaattc attaatctga tattatttaa aatatcgtaa
     4321 atataaaccg gtgaagaatt attataccct gaaactgtaa atacttttga ggttgaatca
     4381 gcaccattga tttgcgcaga taacttttta tctgtcagtg aaaatttacg cggataagaa
     4441 atttcaaaat tgtcaaagta catttctccg ctgaatccgg gctgatgaga atattttaca
     4501 gaaactgtat taacgctgct tccgctgagt agtgatgtcg aaaatgaaac agtggtatct
     4561 attttattaa aatcattaga aagcaaagtg ctgataagag taccgtttat tctgacttca
     4621 agagaatgct cgtttataat ggatgagtta cgggtttttg gaatagcaaa taacttaaca
     4681 gttgaagtct gaggtgaaga gctttgcacg ggtgctgaaa aagtatctgt taaaacttgt
     4741 gtgttgtaca tcaattccca ataccagcct tctcccagaa ctttttctgt tgacaagaat
     4801 ctgtaatcag tagctgctgt attttctccc tgataataaa gtttatcttt ttcaaaatgg
     4861 atcttatccg taaaataatt attcggataa agagtgctga ctgaataatt caaattctga
     4921 aatcttagac cattcgctcc gccccagtcc acccagtaaa catttgtatc agaatactga
     4981 ttataatatt cgtctgtaac atataaaaca ttgttattgg aatcataggt ttttacaagt
     5041 cctccgtagt ttctgtctcc aaaaaaatca aaaaaatcat tatcattaaa tacgccgtcc
     5101 tgctcacctg aaaagtaaat cggtatctgt aatcctttgt tgtaaacttt tactgtccgt
     5161 ggatccagac cggttgtgtt aattccggca ttcgcaaaat cagttctcac tattctgctc
     5221 atacctttat tgactacata cattttcaaa taggtcttgt taggtgtgat ccagtttgga
     5281 tttgtctgtg aaaataaatt tttatttatt gaaaatattg aaatgaataa cataactaaa
     5341 aaaatacatc caaccggaga aagtagtttc ctgtacatcg ctgttttgat attcattatt
     5401 taatatttat ttatttagaa ttagtttctg ctttgctgtc gaatgatcct gaaaattttt
     5461 gaatttactc ttgcaggaga aacgtatgtc attgcattca caatataatt tttcctgttc
     5521 ttccagttgt aaaattcatt aaacatttca tttattatct gcatttcaga tttataatcg
     5581 gataaagtgg cacatgaagt aagaaggaaa tatgtgatcc gttcctgaat ctctttttct
     5641 ttgctgaatt tagaattcct taattgacgt gaaatattca aaattgatct tgctttatct
     5701 tcgatcattt tttttgtatt cctcacaaga gaaatttttt cttttgaatc catatttacg
     5761 taaaacaata tttcaggaac atgtatgata ttattattga ccttagctgc tctgatatca
     5821 tattcataat cttcccatat tcttgatttt gtccagggac caattttatt aacgatctct
     5881 cttctccaga cacaggcacc tgttgcccag ggtcttccat gcatatcaaa aattatcggg
     5941 ataatgctgt catatgtctt gtcatttttg tttcttaaaa agtttccttt gcttctgata
     6001 agtaatgttt tactgtatgc catgccggct gcaggatttt tatccataga attaaccagc
     6061 ttttcaagaa attccttgtg ccagaaatca tccgagtcta aataagcaaa aaaatctcct
     6121 tttgctacaa gtctgccggt ctctctcgac agtcccggtc cgagattttt cgaatttcta
     6181 attagtttta tattgaaatc cgatgaattg tatttttcta tttctttcag atcaaatttt
     6241 tcgctcgaaa agtcatctac taatatgacc tcaattggtt tgtatgtctg attgataact
     6301 gattttatag aagattttag taaatcaact ctgttaaaaa atggtatgat aactgaaacc
     6361 agcttaccgg aattagtatt aaaatccata ttaaatataa atcagtttat aaaaaacact
     6421 ctaaaaaatt tagtccaaag aaaattaaca ggttaattta atgattacag atatactaaa
     6481 aaatgtattt taaatgat
//
LOCUS       DAORVQ010000184         6494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7542_length_6494_cov_5.534866, whole genome shotgun sequence.
ACCESSION   DAORVQ010000184 DAORVQ010000000
VERSION     DAORVQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7542_length_6494_cov_5.534866"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLX80_12060"
     CDS             complement(<1..386)
                     /locus_tag="PLX80_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY21570.1"
                     /translation="MNNLTANTLADTFECVRNLTKFYFSKLDDSQYMTEHKIDNVDIN
                     NAYWLFAHLVWTEHSLIIQGIADQDMEIDWLEKFAYDTDPKSIESPPSLEEIQKQSEL
                     VHLKAMEIIRGLSDEDLEKDNYFNFT"
     gene            524..1516
                     /gene="gap"
                     /locus_tag="PLX80_12065"
     CDS             524..1516
                     /gene="gap"
                     /locus_tag="PLX80_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQY21571.1"
                     /translation="MALKVGINGFGRIGRLVLRRMLAEGGFDIVGINDLTDNKTLAHL
                     FKYDSIHGRFNGDVKLTENGMSINGDEIKITSEKDPTKLGWGDLGTDVVIESTGVFTS
                     KEKLKLHLDAGAKKVILTAPAKDQLDATVVLGVNDSVLTPDMKIVSNASCTTNCLAPM
                     VKVLDDNFKIVKGFMTTIHSYTNDQGLLDQPHSDLRRARAGAINIIPTSTGAAKAVGE
                     VLPHIKGKLDGFALRVPTPDGSLTDFVCVTEKETTKEEVNAAMKKASENEMKGILEYT
                     EDPIVSSDIVGNSSSCIFDSLSTMVSGNLVKVIGWYDNEWGYSCRVVDLIKKIS"
     gene            1544..1807
                     /locus_tag="PLX80_12070"
     CDS             1544..1807
                     /locus_tag="PLX80_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21572.1"
                     /translation="MDTAFLVISLFLPRTTLIIYYLFLQIPMNTVPFIGDILLTVFIP
                     RALIVIYIAQNLGTDSPWFWIHLVTAVLVYFGGGKKYKDYRRK"
     gene            1943..2015
                     /locus_tag="PLX80_12075"
     tRNA            1943..2015
                     /locus_tag="PLX80_12075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1976..1978,aa:Gly,seq:gcc)
     gene            2115..2188
                     /locus_tag="PLX80_12080"
     tRNA            2115..2188
                     /locus_tag="PLX80_12080"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Cys,seq:gca)
     gene            2759..3022
                     /locus_tag="PLX80_12085"
     CDS             2759..3022
                     /locus_tag="PLX80_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21573.1"
                     /translation="MENVNQVFEAVEVIAYFKNLKLEILRFKWNNGVYRVSEMLQSWK
                     IPVREGFVMHYIVTCKEKDLLCELSFSNLDMKWELVRYDELSS"
     gene            3149..3739
                     /locus_tag="PLX80_12090"
     CDS             3149..3739
                     /locus_tag="PLX80_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQY21574.1"
                     /translation="MSVFFKAKSGKFSSSVGILIIRLSLGSLFLFAGAGKLLNLQAFI
                     TSVQEMGQMNDNIAFILAFVLPFMQMIFGAMYIIGLFTPVTSFFIAVMSFSFLIVLGI
                     GHPELPYSYNFVFLACGIATMFMGAGLISFDALIDKEKPESERNINVTPPTVIVRDTS
                     STPMKNENYADEKEVIVTVEESNIKENSGENSGGNN"
     gene            complement(3768..4226)
                     /locus_tag="PLX80_12095"
     CDS             complement(3768..4226)
                     /locus_tag="PLX80_12095"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HQY21575.1"
                     /translation="MTHPSVNEMWNRFIIAHPEFSDSKYTSWYFCNTKKCADDLAVLV
                     NAGIKTATCSLNYWYENGFEKRPEAGEFNVITNWEGEAQFITRTVKLTLMKFKDMTED
                     LASREGEGDRSLEYWRKVHIRYFSDELVKEGLEFNEDLSILFEEFVKVFE"
     gene            complement(4233..5510)
                     /locus_tag="PLX80_12100"
     CDS             complement(4233..5510)
                     /locus_tag="PLX80_12100"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017036415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQY21576.1"
                     /translation="MLDKITVLVGLQWGDEGKGRMVDFLAQEADAVVRYQGGNNAGHT
                     VVNEFGTFKLHLIPSGIFNDKVMNILGTGMVIDIEALSEKLEDLEKNGISTKNIRLSD
                     RATITFPFHRLEDNLEEERLGSAAFGSTRRGIAYAYGERYMKKSIMLGELNYPEHLKK
                     RITDIVEWKNLILKNVYKSDETLNSGEILDWLNEFGGKIKDKICDTSLLLDEYYNDNK
                     KILFEAQLGTLRDIYYGIYPFTTSSCTLATFAPVGGGLPGKRIDNIIGVMKSFSTCVG
                     SGPFVTEMSDEEAGQLRETAMEYGASTGRPRRIGHFDAVATRYGARIQGTDEIAMTKL
                     DSLSGQKKLKICIGYNIDGKSFNDFQLTPGLEIAEPEYIEMDGWDEDITGCRNFTELP
                     KNAQDYVNKVEDLIGFKIKYISVGPERESLIIK"
     gene            complement(5600..5923)
                     /locus_tag="PLX80_12105"
     CDS             complement(5600..5923)
                     /locus_tag="PLX80_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY21577.1"
                     /translation="MSDIVVKYTGEGDEKSVLIEIQNEMLGLPNHRSLNDIVNEEIKN
                     DIKNFCFDLSNLTAISSSGIGVLIGNLKNINSAGGKLKIENANDKILNIFRISKLDSI
                     FNLNG"
     gene            complement(5923..>6494)
                     /gene="secF"
                     /locus_tag="PLX80_12110"
     CDS             complement(5923..>6494)
                     /gene="secF"
                     /locus_tag="PLX80_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQY21578.1"
                     /translation="EELRRNALFAVLFSLLAILIYLAFRFQFVFAMGAVIALFHDVLI
                     TIAVIAIADFAVPGLRLEFNQSMLAAFLTLVGFSSNDTVVVFDRIRENIKLFKNEDTL
                     AIMNKSINATLSRTMITSGIVFLTVLVLFIFGGEVNRSFAFTFGIGIITGTYSSVFVA
                     SAIVTDWKLRTVGLKNKSENFKTAKVKKA"
BASE COUNT         2104 a         1143 c         1071 g         2176 t
ORIGIN      
        1 attgtgaaat taaaataatt atctttttct aaatcttcgt cacttagtcc ccgaataatt
       61 tccatagctt ttaaatgtac taattcggat tgtttttgaa tctcttcaag cgaaggcggg
      121 gattcgatcg attttggatc tgtgtcataa gcaaactttt ccagccagtc aatctccata
      181 tcctgatcag caattccctg tattatcaga gaatgttctg tccatacaag atgggcaaaa
      241 agccagtaag cattgttgat atcaacgttg tcaattttat gttcggtcat atactggctg
      301 tcgtctaatt tggaaaaata aaatttggta agatttctga cacattcaaa agtatcagca
      361 agtgtatttg cagttaaatt attcatgatt tgaatattaa aaattttcct aaaactatgt
      421 aaaaatgatt taatattaaa ggttttaaat tgattctgaa aagcgatttg agtaatttac
      481 aactgcattt tttataaatt taaagagtaa tggaggtaat ttaatggctt taaaagtcgg
      541 tataaatggt tttggaagaa taggaagatt agttcttaga agaatgttgg cagaaggtgg
      601 atttgatata gtaggaataa atgatcttac ggataacaaa actttagccc atttatttaa
      661 atatgattcg atccacggaa gatttaacgg agatgtaaaa ttaacggaaa atggtatgag
      721 cataaacgga gatgagatca agatcacgtc agaaaaggat ccgacaaaac tcggctgggg
      781 agatcttgga acagatgtcg tgatcgaatc gacaggtgta tttacttcaa aagaaaaact
      841 taaacttcat cttgatgcag gagcaaaaaa agtcatactt acagcacctg ctaaagatca
      901 gctcgatgct acagttgtac tcggtgtaaa tgattctgtg ttaactcctg acatgaagat
      961 cgtttcaaat gcttcatgta caactaactg tcttgcacca atggtgaaag ttttagatga
     1021 caatttcaag atcgtaaaag gttttatgac aactattcat tcttatacaa atgatcaggg
     1081 cttactcgat cagcctcaca gtgatctccg cagagccaga gccggcgcga ttaatatcat
     1141 tcctacatcg accggtgcag caaaagcagt cggcgaagta cttcctcata tcaaaggaaa
     1201 gctcgacgga ttcgcgctga gagttccgac tcctgatgga tcacttactg attttgtatg
     1261 cgttacagaa aaggaaacta caaaagaaga agtaaatgca gcaatgaaaa aggcttctga
     1321 aaatgaaatg aaagggatcc ttgaatatac tgaagatcca atagtttcaa gcgatatagt
     1381 aggaaattcc agttcatgca tatttgattc tctctcaaca atggtcagcg gaaatctcgt
     1441 aaaggtaatt ggttggtatg ataatgagtg gggctatagc tgcagagtag ttgacttgat
     1501 caaaaaaata tcttaatttt aaaaaatatt ttaaaataaa aatatggata ctgcattttt
     1561 agttatcagt ctttttttac cccggactac acttataata tattatcttt ttttacagat
     1621 tccaatgaat acagtaccat tcataggcga tatactcttg acggttttta ttccgcgcgc
     1681 actgattgtg atttacatag cacagaatct gggaacagat tcaccctggt tttggataca
     1741 tcttgtaaca gcagtgcttg tttatttcgg cggcgggaag aagtataagg attacagaag
     1801 aaaataattt taagatgtgt ttttttattt taattaagac cgtttctgat tttattttca
     1861 ttaagatatt tttgaatttt taaaaaagta caagtttaag gacttgaata actatgatta
     1921 taaaattatt tttgtaacgt ttgcgggaat agctcagctg gtagagcgca accttgccaa
     1981 ggttgaagtc gcgggttcga gtcccgtttc ccgctctttt ctttttatgt tcgttcgtat
     2041 atattatttt cacattcagc tttaataatt tgaaatgatt gaacatttaa agttcgaaat
     2101 ttaaaattaa atttggtgtg gtacccaagt ggcttaaggg gaaagtctgc aaaactttta
     2161 ttcgacagtt cgaatctgtc ccacacctca ctgcagcagc ctgcaatctg caacccgtga
     2221 ttttcatgca gcgtgttgca atatccaacc atctgctgaa aaaattctct ccaaaaaaat
     2281 ccttatttat aaaaattact catcttactt tacttcagca aataataaaa ggaattatat
     2341 atgagtagaa ttttctttct ctcacatttc acacattaat tgatttttaa atttaatatg
     2401 gagatttaaa atgtttattc aaacacatcc ataagcaaac atcttctgca cttttggcgg
     2461 gtgaacaaaa agattgcata agatttgtag taattttcat atgtcattcc gacaaacttt
     2521 ttgctgtttt ttataaaaga ggatgattcc aatagttatc atgatttata ttttccggag
     2581 taatacattc cattctgcaa gaagaaatta tatatttgaa ggattaaaat ttatgtaata
     2641 tagtttcctt ctattgaagt tttcacttat tattttattc attcgtaatt gtaattatcc
     2701 tttcattgac aataatattt aattacctta aattattatg gtaattaaaa taacagccat
     2761 ggaaaacgta aatcaggtct tcgaagcagt cgaagtgatc gcctacttca aaaatctgaa
     2821 acttgaaatt ctcagattca aatggaacaa cggagtttac agagtatccg aaatgctgca
     2881 aagctggaaa atccccgtcc gtgaaggatt cgtcatgcac tatatcgtga catgcaaaga
     2941 gaaagatctt ctctgtgaac tctctttctc taatctcgat atgaagtggg aacttgtgag
     3001 gtatgatgaa ctttcttcat gatctttgtg cagagaaatt tcggataatt atcactttca
     3061 atattgggta tattaaaaaa gttatttttt ttaaattgta aaagcattga aattatttca
     3121 gtaatttata aaaaataaaa aaactaaaat gagtgtattc tttaaagcta agagtggaaa
     3181 attttcatca tcggtcggta tactgattat cagactttca ttagggtctt tgtttttatt
     3241 tgccggagca ggtaaactgc tgaatctgca ggcatttatt acttccgttc aggagatggg
     3301 acaaatgaat gataacattg catttatact ggcttttgta ctgccgttta tgcaaatgat
     3361 atttggagca atgtatatca ttggtctgtt tacaccggtt acaagttttt tcattgcagt
     3421 tatgtctttc agttttctca tagtattagg aattggacat cctgaattac cgtatagtta
     3481 taattttgtt ttccttgcat gcggaatcgc tacaatgttt atgggagccg gacttatctc
     3541 atttgacgca ctgatagata aagaaaaacc ggaaagcgaa agaaatatta atgtaactcc
     3601 accaactgta atagtcagag atacaagcag tactccgatg aaaaatgaaa attatgcaga
     3661 tgaaaaagaa gtcattgtaa ctgtagagga atctaacata aaagaaaatt caggtgaaaa
     3721 ttccggcggg aataattaat gtttaaataa aatatcagta tgaagattta ttcaaatact
     3781 tttacaaact cttcaaaaag tatggacaag tcttcgttaa attcaagtcc ttcttttaca
     3841 agttcatcag agaaataacg aatatgaact tttcgccagt attcaagtga tctgtcgcct
     3901 tctccttctc ttgatgcaag gtcttcagtc atatctttga acttcattaa agttaatttt
     3961 acagtcctgg taataaactg ggcttcgcct tcccagtttg tgatcacatt gaattctccg
     4021 gcttcaggac gtttttcaaa tccattttca taccagtaat ttaatgagca cgttgctgtt
     4081 tttatacctg cgttaactag cacagccaga tcgtctgcac atttcttcgt attacaaaaa
     4141 taccatgaag tatatttaga atcagaaaat tcaggatgag ctataatgaa tctgttccac
     4201 atttcattaa cagaaggatg tgtcatagta atttatttga tgataagact ttctcgttca
     4261 ggacctacag aaatatattt gatcttaaat ccgatcagat cttccacttt atttacataa
     4321 tcctgtgcat tttttggaag ttcagtaaaa tttctacaac ctgtaatatc ctcatcccag
     4381 ccgtccattt ctatatattc aggctcagca atctcaagtc ccggagtaag ctgaaaatca
     4441 ttaaagcttt taccatcaat attataacca atacatatct taagtttttt ttgtccggat
     4501 agactgtcta attttgtcat tgcgatctca tctgttccct gtattctcgc gccgtatctt
     4561 gtagctaccg catcaaaatg cccaatcctg cgaggtctgc cggtagaagc tccgtattcc
     4621 attgctgtct cccgaagctg tccggcttct tcatcactca tctcagtaac aaacggaccg
     4681 cttccaacac atgtagaaaa tgatttcatc actccgataa tattgtcgat cctttttccg
     4741 ggtaatcctc cgccgacggg tgcaaatgta gcaagagtgc aggaagatgt agtgaatgga
     4801 taaattccgt aataaatatc ccgtagagtt cccagttgtg cttcgaaaag tatcttctta
     4861 ttatcattat aatattcatc taataaaagt gatgtatcgc aaattttatc tttgatcttc
     4921 ccgccaaatt cattcagcca atcgagaatc tctcctgaat ttaatgtttc atcactttta
     4981 taaacatttt tcaaaataag atttttccat tctacaatat ctgtgatacg ctttttaaga
     5041 tgctccgggt aattaagctc accaagcata atactttttt tcatatatct ttcaccatat
     5101 gcataggcaa ttcctcttct tgttgaacca aaagcagcac tgccaagcct ttcctcttcc
     5161 agattatctt ctaatctatg aaatggaaat gtgatagtcg ctctgtcaga aagtcgtatg
     5221 tttttagtac tgataccgtt tttttcaaga tcttcaagtt tctccgaaag agcttcaatg
     5281 tcaatgacca tacctgtacc aagaatgttc atgaccttat cattgaaaat acctgaggga
     5341 ataagatgga gcttgaatgt accaaattca ttgactacgg tatgtccggc attatttcca
     5401 ccctgatatc tgaccactgc atcagcttcc tgagccagaa aatccaccat tcttcctttt
     5461 ccttcatctc cccattgaag acctactaaa actgtaattt tatctaacat taatattggt
     5521 ttggtttaaa taaaaaaatc ttcaatcatt aaatctgaat tgaagacttt ttaaaaatta
     5581 attatatgta ttttgcttat taaccgttta aattaaatat tgaatcaagt tttgagatcc
     5641 tgaaaatatt cagaatctta tcatttgcgt tttcaatctt aagtttacca ccggcactgt
     5701 taatgttttt aagattaccg attagtacac caatgcctga actactgata gctgttaaat
     5761 ttgaaaggtc aaaacaaaaa ttcttaatat catttttgat ctcttcattt acaatatcat
     5821 tgagtgatct gtgatttggc agacccagca tttcattttg aatttcaata agtacagatt
     5881 tttcatcgcc ttcaccagta tattttacaa ctatatctga cattatgctt tttttacttt
     5941 tgctgttttg aaattttcag atttattctt tagtccaaca gttcttaact tccagtcagt
     6001 aacaattgca cttgcaacaa aaacagaaga ataagttcca gtaataatac ctataccgaa
     6061 agtaaatgca aaacttctgt tcacttcacc accgaaaata aataacacca gcacagtaag
     6121 aaaaacaata ccactggtaa tcatagttct gctaagagta gcgtttatac tcttattcat
     6181 gatagcaagt gtatcttcat ttttaaataa tttaatattc tctctgatcc tgtcgaatac
     6241 aaccactgta tcatttgacg agaaaccaac aagtgtaaga aaggctgcaa gcatagactg
     6301 attgaattcc agccttaatc ccggaaccgc aaaatctgct attgctatta cagcaatggt
     6361 gattagaaca tcatgaaaaa gtgctattac agctcccata gcaaaaacaa actgaaatct
     6421 gaatgctaaa tagatcagaa tagccagaag agaaaataaa acagcaaata atgcattcct
     6481 tctgagttct tctc
//
LOCUS       DAORVQ010000185         6458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7611_length_6458_cov_5.542558, whole genome shotgun sequence.
ACCESSION   DAORVQ010000185 DAORVQ010000000
VERSION     DAORVQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_6458_cov_5.542558"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..2695)
                     /locus_tag="PLX80_12115"
     CDS             complement(299..2695)
                     /locus_tag="PLX80_12115"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY21579.1"
                     /translation="MKKNFSFLVVFAFLVTSLLVGFTLSNINDEKPAQRNSKVITTSQ
                     NDLTPVTKDQDYRKRNPFVETPQYSNDGNPNYSQASLSESFEGATFPPTGWTKLNPDA
                     GPGWNRQTAGTTPIPGWNGGDITTPVGGGSAVAFCTWTTGGAVSNDQWLITPQLTGVL
                     STDVLKFSLRYWPNTFRDSIEVLLSTTGNTIPNFTNLIFRKNFAVATTIDTGWINYSF
                     PLSAFAGQNVYIAFREVVADNFNDGASFSLDLVSVGTGSANDIATTSINAPLSVTLPT
                     PTIAPKATYSNVGSAAQTNIPVTFKITGPVNYTSNKVIASLGVGASVTVTFDSTFNPT
                     AGTYNNTVYCSLGSDVNRANDTLKNSFNAVDPNYGTQNGISFANSYATSAPSMPTFCW
                     KDTTGSTSIAVNRVNVSSAPLTGTLDDGYWRIGNALNGKKIRMGGVSYDSFFVATNGM
                     VGFAANSGLTSFTPSLVSTVRPAIYPLWMDMDWRPPTTGGLGSSRLSYKVLDDYQLLI
                     SFDRAQEYNAGTADTGDHVSYQVVVELMDAGATTNSRMVVQIADTVGQKTGSKFLGYY
                     NTNLLNAHVMGVQTAAGDASYYRAAYPLTTYPGPLFGPGGASLAVEYGNNPNILNQGC
                     GGVTLTFNGSIEAMTPSNVGDTVTVQLRSTTSPYEVKDASKKYMNGAGNASFTFSKIP
                     TGTYYIAVRHRNALQTWSAAGVPVAGGLMSYNFKTDIAKAFGSNMVVVSGQASFYSGD
                     VNQDNVIDVTDLGDVDNDANNFISGYVNTDVNNDLAVDVTDAAYVDNNAFNFVGAVIP
                     "
     gene            complement(2872..4710)
                     /gene="glmS"
                     /locus_tag="PLX80_12120"
     CDS             complement(2872..4710)
                     /gene="glmS"
                     /locus_tag="PLX80_12120"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQY21580.1"
                     /translation="MCGIVGYIGNRECLEFIIDGLKRLEYRGYDSAGVAILNEHGNIL
                     SYKKKGKVKELESQFDKDILAGKTGIGHTRWATHGEPNDINAHPQMDMTGNIAIVHNG
                     IIENYKTLKKKLEKENFTFTSDTDTEVLASLIQYHFKIFNDFEKAVRISLNEVEGAYG
                     IAILNKSEPDKIIIARNGSPLMFGLGKDEIIVSSDAAAIINLTKNVIYLEDGEMAVLK
                     KDSFKIKTTKDEEIFREIQRIDYDLEEIEKGGYEHFMLKEICEQPDAIQNTYRGRINF
                     ETGEVKIGGLDSVRDKLINAKRIIITACGTAWHAGLVGEYLLEEYCRIPTEVEYASEF
                     RYRNPIIYDDDVVIFISQSGETADTMAALKEAKRLGATTVGIVNAVGSSIARAVDAGT
                     YIHAGPEIGVASTKAFTCQLMSLILITLMIGEAKGTISQKDKKDIADELKLLPGKINE
                     IIEKKNDFKFIAEEFKDSKNFLYLGRGLNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDENMPVVFIAPKDKTYDKIVSNIEEIKARKGKILTIATQNDEEINLYSDYIM
                     RVPKTLPIFSPILNSIPLQFLSYYIATARGCNVDQPRNLAKSVTVE"
     gene            complement(4813..4898)
                     /locus_tag="PLX80_12125"
     tRNA            complement(4813..4898)
                     /locus_tag="PLX80_12125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4862..4864),aa:Leu,seq:caa)
     gene            5220..>6458
                     /locus_tag="PLX80_12130"
     CDS             5220..>6458
                     /locus_tag="PLX80_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQY21581.1"
                     /translation="MGKPDYRFGDIPKRISNTIKIAFLQTKLLRSKLAGILHLCIYWG
                     FVVLLLVVVESIIEGFFPEFSFSFLGPFYNVITFLQDFFGALVLVTVIFSLIRRYIGT
                     PKRLQVERAAKLDATFILVMIAIVMITMFGMSVERILLGASEGYRPVSAFIAGFFTTG
                     SEPAYEFFFWAHIIVVLGFLNYLPYSKHFHVLSSVPNTFFSNYEIPYQGIVLKPINFE
                     DESIVQYGAKDVEDLTWKNLLDGYTCTECGRCTSVCPANITGKLLDPKLIITKIRKRT
                     SEKGPAVIGKKEEDPALNKNLVHDYISSQELWACTTCMACVQECPVMIDHVTPIVDMR
                     RDLVMMESDFPAEMNTVFRNLENNESPWAFGAEQRNDWIDDLSDELAKEGKENHLKKL
                     SNIGSADELDVVFWSGCAGAF"
BASE COUNT         1916 a         1212 c         1074 g         2256 t
ORIGIN      
        1 actatttact atttactatt tactatttac tatttactat ttactattta ctatttacta
       61 ttaattatta actattaact attaactata cttccacgga aaaatgctcc ggcaagaaat
      121 gtaggaacac cggtcacaat gctgatattg aaaagtgctt tattaatagt ggtaccggat
      181 ccggtaatta attaattttg attataatca ataaaccaaa tataaaaaaa aaagcagggt
      241 aaaaaattta ccctgcttga tttgtgtact aaagatctgt ttattttgct ttaaattatt
      301 atggaattac tgcaccaaca aaattgaaag cattattgtc aacatatgca gcatccgtaa
      361 catcaactgc taaatcattg ttaacatcag tattaacata accagatata aaattatttg
      421 cgtcattatc aacatcacct aaatctgtaa cgtcaataac attatcctga ttgacatctc
      481 ctgaatagaa acttgcctgt ccggatacaa ctaccatatt acttccgaaa gcttttgcaa
      541 tatctgtttt gaagttgtag ctcattaatc cgcctgcaac aggtacacct gcagcactcc
      601 atgtctgaag cgcatttctg tgtcttactg caatataata agttccagtt ggaattttag
      661 aaaatgtgaa tgaagcattt cctgctccgt tcatatattt tttagaagca tcttttactt
      721 catatggtga agttgtgctt cttaactgaa cagtaactgt atcacctaca tttgaaggtg
      781 tcatagcttc aatggatccg ttaaaagtaa gagtaacacc gccgcatccc tgattcagaa
      841 tatttggatt gttaccgtat tctactgcaa gcgaagctcc gcccggtccg aataatggtc
      901 ccggataagt tgtcaatgga tatgctgctc tgtaataaga tgcatctccc gctgctgtct
      961 gaactcccat tacatgtgca ttaagtaaat tggtgttata gtatcccaga aatttacttc
     1021 cagttttctg accgactgta tctgcgatct gaactaccat tcttgaattt gtagttgcac
     1081 ctgcatccat taattcaaca acaacctgat aactaacgtg atcacctgta tctgcagtac
     1141 ctgcattata ttcctgtgct ctgtcaaaag agatcagaag ctgataatca tctaatactt
     1201 tataacttaa tctgctggat ccaagtccgc cagttgtagg cggtctccag tccatatcca
     1261 tccataatgg atagattgct ggtctgacag tagataccag tgatggtgtg aatgaagtta
     1321 aaccggaatt agcagcaaaa ccaaccattc cgttagttgc tacaaagaat gaatcataag
     1381 aaactccacc cattctgatt tttttaccat taagtgcatt tcctattctc cagtatccgt
     1441 catctaaagt acctgttaat ggagcagagg atacattcac tctgttaact gcaatacttg
     1501 tacttcctgt agtatctttc cagcagaaag taggcattga tggtgcactg gttgcatagc
     1561 tgtttgcaaa ggaaattcca ttctgtgttc cataatttgg atcgacagca ttaaatgaat
     1621 ttttaagagt gtcgttagct ctgtttacat ctgacccaag actgcaataa actgtgttgt
     1681 tgtatgtacc ggcagtcgga ttaaatgtac tgtcaaatgt tacagtaact gaagcaccta
     1741 cacctaatga agcaataact ttgttacttg tgtaattaac cggacctgta attttgaatg
     1801 ttacaggtat gtttgtctgt gctgcagatc caacattcga ataagttgct tttggtgcaa
     1861 ttgttggtgt tggtaatgtt accgataatg gtgcattaat tgaagttgta gcaatgtcat
     1921 ttgcagaacc tgtccctaca gatactagat caagtgagaa tgaagcaccg tcattgaaat
     1981 tatctgctac tacttctctg aatgctatat aaacattctg accagcaaat gcacttaatg
     2041 ggaatgaata attaatccat ccggtatcaa ttgttgttgc aactgcaaag ttttttctga
     2101 atattaaatt tgtaaaatta ggaatagtat taccagtagt tgaaagaaga acttcaatac
     2161 tgtctctgaa tgtatttggc cagtatctta atgaaaattt caacacatca gttgacaaga
     2221 cacctgtcag ttgaggagta ataagccact gatcattact gactgcacct cctgtagtcc
     2281 atgtacaaaa agcaacagct gatccaccgc caaccggggt agtaatatct ccgccgttcc
     2341 aacccgggat aggagtagtt ccggctgtct gtctgttcca gcccggacca gcgtcaggat
     2401 ttaattttgt ccagccggtc ggagggaaag ttgctccttc gaaagactct gataatgaag
     2461 cctgagagta attcgggtta ccgtcatttg agtactgtgg tgtttccaca aaaggatttc
     2521 tttttctgta atcttgatcc tttgtaaccg gggtcagatc attttgactg gttgtaataa
     2581 ctttagaatt tctctgagca ggtttttcat cattaatatt acttaatgta aaacctacta
     2641 ataaagaagt tactaaaaaa gcaaaaacaa ccaagaatga aaaatttttt ttcatttcat
     2701 ttcctttctt ttggtttaat tttttaattg gtttttggaa ctgttaaaat aagtattttt
     2761 gttgtattat tcaatttttt tttcaataaa aaatatataa atgcgaatcc ccctttagac
     2821 tgttaaatca aagggggatt ttctctttgt taaaataatt tttagaagtt tttactccac
     2881 tgtaacactt tttgcgagat tcctcggctg atctacatta caacctcttg ctgttgcaat
     2941 ataatatgat aaaaactgaa gtggaatact gtttaaaata ggtgaaaaga tcggcagagt
     3001 ctttggtact ctcattatat aatcagaata aagatttatc tcttcatcat tttgtgtagc
     3061 tatagtaagg atctttcctt ttctggcttt gatctcttct atatttgaaa ctattttgtc
     3121 ataggtttta tcctttggag caataaaaac caccggcata ttttcatcta tcagtgctat
     3181 cggaccgtgt ttcatctctg ctgccggata accttcagca tgaatgtaag aaatttcttt
     3241 cagcttcaat gcaccttcaa gagcaacagg gaaattcaat ccccttccga gatataagaa
     3301 attttttgaa tctttaaatt cttcggctat aaatttaaaa tcatttttct tttctatgat
     3361 ctcatttatt ttaccgggta ataatttcaa ctcatctgct atatcttttt tatccttttg
     3421 tgaaatggtt cctttagctt ctcctatcat taatgttatt aggattagag acataagctg
     3481 acatgtaaaa gcttttgtag atgcaactcc tatttcaggt ccggcgtgaa tgtaggttcc
     3541 tgcatcaact gctctggcga ttgaacttcc gacagcattt acaattccta ccgttgtagc
     3601 tccaagtctt ttggcttctt ttaaagctgc cattgtatca gcggtttcac cactctgact
     3661 tataaatatt actacatcgt catcataaat gattggattt ctgtatctga actctgaagc
     3721 atattctact tcagtaggta ttctgcaata ttcttctagc agatattccc ctaccagacc
     3781 cgcatgccat gcagttccgc aagcagtgat gatgatcctt tttgcattaa ttagtttatc
     3841 ccttacactg tcaagaccgc ctatttttac ttcacctgtt tcaaagttga ttcttcctct
     3901 gtatgtgttt tgaattgcat ccggctgttc acaaatttcc tttagcataa aatgttcata
     3961 tcctcctttt tcaatttctt ccagatcata atctatcctc tgtatctccc tgaatatttc
     4021 ttcatctttt gtagtcttga tcttaaaact gtcttttttc aatactgcca tttctccgtc
     4081 ttccagatat atcacatttt ttgtaagatt tattattgct gctgcatcag atgaaacaat
     4141 gatctcatcc ttacctagtc caaacatcag cggacttccg tttcttgcaa taattatttt
     4201 atccggttcg gatttattta aaatggctat tccgtaagcg ccttctactt cattaagaga
     4261 aattcttaca gctttctcaa aatcattaaa tattttgaag tgatattgaa tcaggcttgc
     4321 aagtacttca gtatctgtat cagaagtgaa agtgaaattt tccttctcta attttttctt
     4381 taatgttttg taattctcga taattccatt atggactatt gcaatattac ctgtcatgtc
     4441 catttgcgga tgagcgttta tgtcatttgg ttcaccgtga gtcgcccatc ttgtgtgtcc
     4501 aatacctgtc ttgcctgcta aaatatcttt atcgaactgt gattcaagct cttttacttt
     4561 accctttttt ttatatgata aaatattacc atgctcgttt aagattgcaa cacctgcaga
     4621 atcatatccg cggtattcaa gtcttttaag tccgtctatt ataaattcca gacattccct
     4681 gtttccaata taacctacta ttccgcacat ttttttactc cttaagttaa gttagttaaa
     4741 taagtaatat taaggttgta taagcttagt taatttatta aataaattta taaatatatt
     4801 ttttaatgca agtgccgaaa aagggagtcg aacccttacg agatttgagc ctcgctggat
     4861 tttgagtcca gtgcgtctac cagttccgcc atttcggcaa atagatcttc tcactaaaat
     4921 ttaacgggta gaaattaagt atcataaaaa gtttattcaa catattaatt tctctgaaat
     4981 ttaacagtaa aaaatttctc aataaaaaat ttcaataaaa atacttaaat cttttttatc
     5041 aaaagacaaa aaaatcatca caattatctt taatatttga atctatttaa ttaatttgag
     5101 tgcttgaatt acaaaacata tatctcttaa tgctgagacc tttaatattt ggtttaatct
     5161 ttttcgggtc gatcggtttt tttatttact caatcagtaa atatctttct tatataaaaa
     5221 tgggaaagcc ggattacaga ttcggcgata ttccaaaaag gatcagcaat actattaaaa
     5281 tagcattcct tcagactaaa cttttaagaa gcaagctcgc cggtattctg catttatgta
     5341 tttactgggg ttttgtagtt cttttattag ttgtcgttga atccattata gaaggtttct
     5401 tccctgaatt ttcattttca ttcctcggac cattttataa tgtcattact ttccttcagg
     5461 atttctttgg agcactcgtt cttgtaactg taattttttc tctcatcaga agatatattg
     5521 gtacgccaaa aagacttcag gtcgaaagag ctgcaaaact ggatgctact tttattcttg
     5581 taatgatcgc aattgtaatg atcacaatgt ttggtatgag cgtggaaaga attttactcg
     5641 gtgcatctga aggttacaga ccggtgtcag ctttcatcgc agggtttttc acaaccggtt
     5701 cagaacccgc atatgaattt ttcttctggg cgcatataat tgttgttctg ggatttttaa
     5761 attatctgcc ttactcaaaa cattttcatg tactctcttc agttccaaat actttctttt
     5821 caaattatga aataccttat cagggcatag tattaaagcc gataaatttt gaagatgaat
     5881 ccattgttca atatggcgca aaagatgtag aagacctgac atggaaaaat ttactcgacg
     5941 gatacacatg tactgaatgc ggaagatgta catcggtctg tccggcaaac attacaggaa
     6001 aacttcttga tccaaaactc attattacaa agatcagaaa acgtacatct gaaaaaggac
     6061 ctgcagttat tggtaaaaag gaagaggatc ccgcattaaa taaaaatctt gtacatgatt
     6121 acatttcttc tcaggagctt tgggcttgca ctacatgcat ggcctgcgtt caggaatgtc
     6181 cggtgatgat agaccatgtc acacctattg ttgatatgag aagagatctt gtaatgatgg
     6241 aatcagactt ccctgccgag atgaatactg ttttcagaaa tcttgagaat aatgaatctc
     6301 cgtgggcatt tggtgctgag cagagaaatg actggataga tgatcttagt gatgagcttg
     6361 caaaagaagg gaaagaaaat catttaaaaa aattatcaaa tatcggaagt gctgacgaac
     6421 ttgacgttgt attctggtca ggctgcgcag gtgctttt
//
LOCUS       DAORVQ010000186         6418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7690_length_6418_cov_5.546283, whole genome shotgun sequence.
ACCESSION   DAORVQ010000186 DAORVQ010000000
VERSION     DAORVQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7690_length_6418_cov_5.546283"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..4534
                     /locus_tag="PLX80_12135"
     CDS             56..4534
                     /locus_tag="PLX80_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQY21582.1"
                     /translation="MYWLDWGGALGTRYAQFNYPVGTNYSGVSFSEVKTFDKNYFYSQ
                     GENISSTDYRYLSTERFQGEGWYWATLNDNQTLTETFTLPYLSPTVTTASIYVFAYPR
                     VRNTSILNEHNLEVKVNGNVVAYMTKNDFIRFDSTITFSSSLLVTGTNTVIVKYIPTA
                     ATSGGVYFDKFKINYPRKFTFENAIVSSNLSSSVDTTTKKFNISGFNSVNPVNIYDVN
                     NFKKISGNTFIADTLKFSAKSNSNFQIVNNNITKKPFRIKQKQVPNLVSSSNGADYLI
                     IYNKIFDAQAEQLRSYRQTKDSFRSFKADIEDLYDIFNYGIEDPVAGRNFVKYVYDNW
                     QLPKLGYVCLFGRGSLDPKKYLGSSTFSNNLIPVYGNPPSDGYYTNLNIGTFCYYPMI
                     SVGRLPAYYASEAQSMVNKIIAYENEPPAKWSKDFVFITGGGTTNEQNGHILKSNYDI
                     GVFVDLPPIYGNPIRIFRTDIPGTITFNIKDSIKNTIDRGALFVNFRGHAGSHDWEIA
                     MNDPNTLSNGNKLPIILSLTCFTGENSKADFRGFGERFVYLPDKGAIGFVGTTGWSYS
                     QFGNDYGSFILESMKNDTARRMGDLTKYANKKMSKDSLSFSIRHTLNCYSLIGDPAAK
                     LNLPKTPEHSITSSDYKLSNNYPGVSENLTFTAYPKNYGLNSDSLKIRFEVYKNNQVY
                     YAKDTIRRNFRNLDSIAYTFKVDTAGIYNMLVKLDQGNWTPGEIKTNNNLSVDIPVKN
                     IAFMPLKPVDNSVISTDSVEFTCLNPLVKTSMNSVKVILQMDTTTNFNSAVLKTFANS
                     SISSAYTKFKTSVPLLVNNKLYYWRSNSIINGDSTGWSGIQKFTYIPNLEYVKDNSNK
                     QIELLTNIEILKKQSSQFPSYDLNGVTYKNNGLQNGDYNGNLFVRSYGSNGEEASYFS
                     VHNKNIYIDAGKNQGLNILKVKKLNGKILDFKNLKMNTNSSNDSLLTYLNSFDSTQYI
                     MLLNAAYVPSGKLLAANVKTKLREFGSIYCDSIGYMSYFHSWSFIGYLGASNKEASEG
                     FDPCCRVNITCLDCDHWSEAVSSMDVTFKYSDGTVSNIIGPAQNWGSLSWIQTLNTGS
                     TIKFDVYGIANNNSQVLLYPNVTSNNFTDLSSIDAYNYPRLNIVAKISIDTMTGNNSS
                     ILNSVKVTYTPPAEPVWDINTIFVNSNYKAGDYLKASGDLYNPGFLNLEGMVVKFYKN
                     SVTPVNLLEVDTVKYAINIDNFKKYEYKLRLPFFRDSIGIFMNVTPVGSNNEFFTFNN
                     TAYIEVKDGYHYRPGSSNIAVYSDGLILKNGDYVRKDPEIKIEITDVDNFIPFSADTS
                     QLTMKLNGRNIPYYQKGAPSQMVKFADNDKSNSQNNRSIIFYPELKNGGNRLSVYFNK
                     NGVTDSSGFDVLVSEELVIENIYNFPNPMKNETNFMFNIGGLAPPDNFKIRIYTVSGR
                     LIKELEYPVSIGFNQIPWDGKDMDGDLIANGTYLYKVVMEGNTSNESLVQKLVMLK"
     gene            complement(4598..5122)
                     /locus_tag="PLX80_12140"
     CDS             complement(4598..5122)
                     /locus_tag="PLX80_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQY21583.1"
                     /translation="MNEKNNNLETTKVVNKNISDITVIDMDGNKVKLSDYKGKVLMIV
                     NVASKCGFTSQYKGLEEIYNRYKDQGFEILAFPCNDFGDQEPGSNDEIRTFCESKYNV
                     TFKLFDKVNVIGDDKNVLYQRLTENSDPAGDISWNFEKFIIDKNGNITERFKSKVEPE
                     SGEVTSVIEARLSE"
     gene            complement(5234..>6418)
                     /locus_tag="PLX80_12145"
     CDS             complement(5234..>6418)
                     /locus_tag="PLX80_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQY21584.1"
                     /translation="VKKVPTIGVMLKPIGDIFIRLLTMIAVPLVLSSLIVGAASLSDL
                     KHIAKIGGKTIGFYAITAVIAILIGLFFANVIQPGKFMPEENKERLMETFQEDAQSKI
                     DQNVSMNIVDFLVNIVPKNPFKAMADGEFLQIVFFAILMGIFLSQINADASKTVLKFF
                     DGVSKTMILIVEKIMIIAPYAVFTLISATVAEFGFNILQTLLLYSFTVIIGLAVLTFI
                     EYPLLLKLFTKVNVVKFFKSQRQVIAVAFSTSSSAATLPVTMDICEKKLGVPNRIASF
                     VLPLGTTINMDGTALYQAVAAVFIAQVYGFDLTLTQQMTIVITAALAAIGTAPVPGIG
                     LIMLIIVLRSIGVPSEGIALIIGVDRLLDMCRTVPNVIADSLACVVIASSEGELGELK
                     VD"
BASE COUNT         2180 a         1071 c         1071 g         2096 t
ORIGIN      
        1 caataattta ttttttacaa ccgatgaata ttataatgaa tatactgata ccaatgtgta
       61 ctggttagat tggggtgggg cacttgggac aagatatgct cagtttaatt atcctgtcgg
      121 tacgaattat tccggtgtat ctttttctga agtaaaaact tttgacaaaa attattttta
      181 cagtcagggt gaaaatataa gttctacaga ttacagatat ctcagtactg aaagatttca
      241 aggcgaagga tggtactggg ctactcttaa tgataatcag actttgacag aaacctttac
      301 tctcccctat ctcagcccga ctgttacgac agcatctatt tatgtatttg cttatccaag
      361 ggtaagaaat acttcaatat tgaatgaaca taatcttgaa gtaaaagtga acgggaatgt
      421 agttgcatat atgactaaaa atgattttat aagatttgac agtactatta cgttttcaag
      481 ttctttactc gtcacaggta caaatactgt aatagtaaaa tatataccaa cggctgctac
      541 ttcaggcggt gtatattttg ataaattcaa aataaattat ccgagaaaat ttacttttga
      601 aaatgcaatt gtctcttcaa atttatcttc atctgtagat actactacaa agaaatttaa
      661 tataagcggt tttaactctg taaatcctgt taacatctat gacgtaaata atttcaagaa
      721 aatttccggt aatactttta tcgctgatac attaaagttt tcggcaaaga gtaattccaa
      781 ttttcaaatt gtaaataata acattacgaa aaaacctttc aggattaaac agaaacaggt
      841 tccgaatctt gtttccagtt ctaatggtgc agattattta attatttata acaaaatatt
      901 tgacgctcag gctgaacagc tcagatcata cagacagact aaagacagct ttcgttcatt
      961 caaagcagat attgaagacc tgtatgatat ttttaattac gggatcgagg atccggtagc
     1021 cggcagaaat tttgtaaaat atgtttatga taactggcag cttccgaagt taggatatgt
     1081 ttgtttattc gggagaggtt cattggatcc caagaagtat ttaggaagtt ccacttttag
     1141 taataatcta atacctgtat atggtaatcc gccatcagac ggatattata caaatttaaa
     1201 tatcggtacg ttttgttatt atccgatgat atctgtcggt agactgcctg cttactatgc
     1261 ttccgaagcc cagtctatgg taaataaaat aattgcatat gaaaacgaac ctcctgcaaa
     1321 gtggtctaaa gattttgtat tcatcacagg cggaggtact actaatgaac aaaacggtca
     1381 tattctgaaa tcaaattatg acattggagt ttttgtagat cttcctccta tctatggtaa
     1441 tccaatcaga atattcagaa cggatatacc cgggacaatt acttttaata taaaagactc
     1501 tatcaaaaat acaattgaca gaggagcttt gtttgttaac ttcagaggac atgcgggaag
     1561 tcatgactgg gaaattgcaa tgaatgatcc gaatacactt tcaaatggaa ataaacttcc
     1621 gattatttta agtcttactt gtttcacggg agaaaattct aaagctgatt tcagaggttt
     1681 tggagaaaga tttgtatacc ttccggataa aggagcaatt ggttttgtcg gaactacagg
     1741 ctggagctat tctcagtttg gaaatgatta tggatctttt attcttgaat ctatgaaaaa
     1801 tgatacagca agaaggatgg gtgatctgac aaagtatgcc aacaaaaaaa tgagcaaaga
     1861 ttcattgtca tttagtatca gacatactct taactgctat tctctaatag gagatccggc
     1921 agctaagtta aatcttccaa aaactcctga acattcaatc acttcatcag attataaatt
     1981 gtcaaataat tatccgggag tttcagaaaa tcttaccttt accgcttatc caaaaaacta
     2041 cggactgaat tctgattcat taaagatccg ttttgaagta tataaaaata atcaggtcta
     2101 ttatgcaaaa gatactatca gaagaaattt cagaaattta gattcaattg cttatacttt
     2161 taaagtcgat actgcaggta tatacaacat gctcgtaaag ctggatcagg gtaactggac
     2221 tccgggtgaa attaaaacaa acaataacct gtctgtagat atacctgtaa agaacattgc
     2281 ttttatgcct ttaaaaccgg tagataattc tgtcataagt actgacagcg ttgaatttac
     2341 ctgccttaat ccgctggtaa aaacttcaat gaattctgta aaagttattt tacaaatgga
     2401 tacgactaca aatttcaatt cggcggtttt aaaaactttt gctaacagca gtatctcaag
     2461 tgcctacact aaatttaaaa catctgtacc attattagta aataacaaac tttattactg
     2521 gagatcaaac agcattataa acggtgacag caccggctgg tcaggtattc agaaatttac
     2581 atatattcca aatttagaat acgtcaaaga taattcaaat aaacaaatag aattactaac
     2641 taacattgag atattaaaaa aacagagttc acaattccct tcatatgatc taaacggagt
     2701 aacttataag aataatggtc ttcagaatgg tgattacaat ggaaatcttt ttgtaagatc
     2761 atatggaagc aatggtgaag aagcttcata tttcagtgtt cataataaaa atatttatat
     2821 tgatgcaggt aagaatcagg ggctgaatat tttaaaagtt aaaaagttaa acggcaagat
     2881 tttagatttt aaaaatctaa aaatgaatac taattcatca aatgattcat tattaacata
     2941 tctcaattcg tttgattcaa ctcaatatat aatgctgctt aatgctgctt atgtaccaag
     3001 tgggaaatta ttagcagcaa atgttaaaac caaactcaga gagtttggaa gtatttattg
     3061 tgattccatc ggatacatga gttattttca ttcatggagt tttatcggat atttaggcgc
     3121 atcaaataaa gaagcatctg aaggatttga tccctgctgc cgtgtaaaca tcacttgtct
     3181 tgattgtgac cactggtcag aagctgtaag ttccatggat gtgacattta aatattcaga
     3241 cggtactgtt tctaatatta taggtcctgc tcaaaactgg ggaagtcttt catggattca
     3301 gactttaaat acgggaagta ctataaaatt tgatgtttat ggaattgcaa ataacaattc
     3361 tcaggtactt ctttatccga acgttacttc aaacaatttt actgatctta gttcgataga
     3421 tgcatataat tatcccagat taaatattgt tgcgaaaatt tctattgata ctatgacagg
     3481 aaataattcc agcatcctga attcagtcaa agtcacatat actcctccag ccgaacctgt
     3541 atgggatata aatactattt ttgtaaattc aaattataaa gccggtgatt atttaaaagc
     3601 atcaggtgat ctttataatc cgggatttct taatctggaa ggaatggtag taaaatttta
     3661 taaaaattct gttacacctg ttaatttgct ggaagtcgat acagttaaat atgctataaa
     3721 tattgacaat ttcaaaaaat atgaatacaa attgagattg ccgtttttca gagacagtat
     3781 aggaattttt atgaatgtaa ctcctgtagg ttctaacaat gaattcttta cattcaataa
     3841 tacagcttac atagaagtta aagatggata tcattaccgt ccgggcagca gcaatatagc
     3901 agtttacagt gatggattaa ttctcaaaaa tggagactat gtaaggaaag atcctgagat
     3961 aaaaatagaa attacagacg ttgataattt cattccattc agtgcagata cttcgcagtt
     4021 aactatgaaa ttgaacggaa gaaatatacc ttattatcaa aaaggtgcac caagccaaat
     4081 ggttaaattt gcagataatg ataaatcaaa ttctcaaaat aaccgctcaa taatttttta
     4141 tcctgaactg aaaaatggcg gcaacaggtt gtcggtatat tttaataaaa atggagtgac
     4201 tgattcttcc ggatttgatg tattagtaag tgaggaactc gtaattgaaa atatttataa
     4261 ttttccgaat cctatgaaaa acgaaactaa ttttatgttt aacataggag gtcttgctcc
     4321 gccggataat ttcaagatcc ggatctatac tgtttcggga agactcataa aggaactgga
     4381 atatcctgtt tcaattgggt tcaatcagat tccatgggat gggaaagata tggatggtga
     4441 tttgattgca aatggaacat atttatacaa agttgtaatg gaaggaaata caagcaatga
     4501 atcacttgtt cagaagcttg taatgttaaa ataatttcaa tagaatttct tattgaaaaa
     4561 agcgaagata taatgtcttc gcttttttta ttttatttta ttccgaaagt ctggcttcta
     4621 ttacagaagt aacttctcct gattcaggtt ctactttact tttaaatctt tcggtaatgt
     4681 ttccgttttt atctatgata aatttttcaa agttccagct gatatctcct gcaggatctg
     4741 aattttctgt cagtctttga tacaatacat ttttatcatc tccaattaca tttaccttat
     4801 cgaaaagctt aaacgtgaca ttgtattttg actcacagaa agttcttatc tcatcattgc
     4861 tacccggctc ctgatcacca aaatcattac atggaaaagc taatatctca aagccctgat
     4921 ctttatatct attataaatt tcttcgagac ctttgtactg tgaagtgaat ccgcattttg
     4981 atgcaacatt tactatcatc agaacttttc ccttatagtc tgataattta actttatttc
     5041 cgtccatatc aataacagta atatctgaaa tgtttttatt cacaacctta gttgtttcca
     5101 aattattatt tttctcattc atattatttg atttaatttc tgctgaatcg gaacatccgg
     5161 catatatgca gacattcgtc agtaaagtta ttgccaaaat tattttaaac atgtttttat
     5221 tttttaattt attttaatca acttttaatt caccaagctc accttcactg ctcgcgatca
     5281 caacacatgc aagagagtca gctataacat tcggcacagt cctgcacata tcaagaagtc
     5341 tgtccactcc gataatcaga gcaattcctt ctgaaggtac acctatagat cttagaacta
     5401 ttatcagcat tatcagacca atacccggca caggtgcagt accaattgct gcaagtgcag
     5461 cagtaattac aattgtcatt tgctgagtaa gagttaggtc aaatccataa acctgcgcaa
     5521 taaacacggc ggctaccgcc tgataaagtg ctgttccgtc catgttaatg gttgtaccca
     5581 aaggaagtac aaaacttgca attcggtttg gcacgccaag tttcttttcg cagatgtcca
     5641 ttgtaacagg caatgtagct gcagaagaac ttgtagaaaa agcaactgca attacctgtc
     5701 tttgagattt gaaaaatttc actacattca cttttgtaaa taacttcagc agcaaaggat
     5761 attctataaa tgttaacaca gctaatccaa ttataactgt aaaagaatac aacagcaatg
     5821 tctgaagtat attaaaacca aattccgcga ctgttgctga tatgagcgtg aatacagcat
     5881 aaggagcaat gatcattatt ttttcaacta taagaatcat tgttttggat actccgtcaa
     5941 aaaatttcaa gacagtcttt gaggcatctg catttatctg agacaagaat attcccatta
     6001 atattgcgaa aaaaactatc tgtaaaaatt ccccgtctgc cattgctttg aaaggattct
     6061 tcggtacgat atttacaagg aaatcaacta tattcataga tacattttga tctattttac
     6121 tttgggcatc ctcctgaaat gtttccatca gacgttcttt attttcttca ggcatgaact
     6181 tcccgggctg tatcacattt gcaaagaaaa gtccgatcaa aattgctatt actgccgtta
     6241 ttgcataaaa tccaattgtc tttccgccga tctttgcaat gtgtttcaga tcagaaagtg
     6301 aagctgctcc tacaataagc gatgaaagca caagcggaac tgcgatcatt gtcaaaagtc
     6361 tgataaaaat atcacctatt ggtttcagca tgaccccaat ggtagggact tttttgac
//
LOCUS       DAORVQ010000187         6374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7771_length_6374_cov_5.238962, whole genome shotgun sequence.
ACCESSION   DAORVQ010000187 DAORVQ010000000
VERSION     DAORVQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7771_length_6374_cov_5.238962"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1477)
                     /locus_tag="PLX80_12150"
     CDS             complement(287..1477)
                     /locus_tag="PLX80_12150"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQY21585.1"
                     /translation="MPFYYKLGQIPQKRHTQFRQPDGSLYKEELLSTIGFDSISTNAY
                     HINSPAKIKDISPDVEIVKIEEWKESSLRPYHFRTKPMNHEGDAFSGRKPVMFNNDVV
                     MSVCRVKDQMNYFYKNALCDEVIFVHEGKGTLQSNLGYMKFREGDYIVIPRGVIYKLV
                     HETEDARYLIFESSGPVSTPHRYRNEHGQLMEHSPYCERDIKCPEEMVTFDEKGDFRM
                     KIKKGEKVVTFHYEFHPFDIVGWDGYYYPWIFNIEDFMPITGKIHMPPPIHQTFAAPG
                     FVICSFCPRMLDYHPLAVPIPYNHSNLDSDEMLYYVDGNFASRKGVDFASISLHPNGI
                     PHGPHPGTVEANLGKKETLETAVMMDTFRPLKLAKFAKEVNDPSYYLSWNSDDPVELI
                     NEGQ"
     gene            complement(1624..2622)
                     /gene="trxB"
                     /locus_tag="PLX80_12155"
     CDS             complement(1624..2622)
                     /gene="trxB"
                     /locus_tag="PLX80_12155"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQY21586.1"
                     /translation="MEISTNGTTQNHHKVIVIGSGPAGLTAALYSGRANLDPIIFEGS
                     QPGGQLTITTEVENYPGFEHGIQGPELMDIMRKQAHRFGAKSIYRNITKVDFSSRPHK
                     ITDEENNEYTTDTVIISTGASAKWIGLESEQTYMGYGVSSCATCDGFFFRGMKVVVVG
                     GGDTAMEEANYLTRHASEVFLIHRREEFRASKIMLDRAKANPKIKFILNTVVEEILGK
                     TEDNKKSVTGARIKNVITGEVSEIECAGFFTAIGHEPNSKIFNGILDMNEYGYLITKK
                     STTETNVEGVFACGDVQDDYYRQAVTAAGTGCMSAIDAERYLEKIEFEETSKANDQ"
     gene            complement(2688..4403)
                     /locus_tag="PLX80_12160"
     CDS             complement(2688..4403)
                     /locus_tag="PLX80_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21587.1"
                     /translation="MHILTNINLKYILLFCILTFLNTSESDLFSQSQKRGSVSDNDKA
                     MFYFVEAKTLELKSDYIGAVENYRNALEFDKSPGIYYSLALVYFKMNKIDESLTEIKK
                     ALNLKPNDQDFIEISANLYIVKKQFVKAIAAYEQIISLDSNYTYGLYSLARLYQETNM
                     PANALAVYEKITAKIGYDFDVLNKMYEIYIANKDYANASDVLESMLKIDPFNANIKKI
                     LANLYLNNKDYDKARVIYENILSLDHTEKEVQNELVKIYFKLNENDKAFENFSKVLGK
                     DSLNFLEKVQVGELYFNLISQDESAKDISKNIFIGIKSEYPDEWIPYYYLGVFDLLDK
                     NESAYKENFETALEMADTARDVFINIGFSLYQEGLTDEAMPVIDQGLSKFPDDFRLNF
                     IKGLALQGTGRESESVPYYEAALDANPADINTLSSLALIYDNQKRFDKSVETYENALK
                     IDPYNALILNNYAYNLSERDVELEKALYMSKIAVEKEPGNASYLDTIGWIYFKMKKYK
                     KAEEFIGKSLDVNPASAVVLEHMGDVYSKLKDSNKAVKYWKMSLEINPSNETLKDKIY
                     SSQVG"
     gene            complement(4555..4989)
                     /locus_tag="PLX80_12165"
     CDS             complement(4555..4989)
                     /locus_tag="PLX80_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21588.1"
                     /translation="MNITLRKINEDNWRECIALTVNDDQLTFVGSNVNGLALAYAHKE
                     MNPLAIYNDETMVGFLMYAKDPDDGVYYINRLMIDRKFQGNGFGKQALSELLNLLRDL
                     KVESVDILHKPDNHKAIKVYESLGFILTEDKVGDEVVSVLKL"
     gene            complement(5167..>6374)
                     /locus_tag="PLX80_12170"
     CDS             complement(5167..>6374)
                     /locus_tag="PLX80_12170"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HQY21589.1"
                     /translation="NLKMMLTALILFSAVIFSSCSNEDSNSLLLSPNSTGSSNAPDLS
                     EEGKNPDELDLSEENDHRNPRGYIYLQSNDATQNSILVFKQNLNGSLILQSTISSGGI
                     GSGGGLGNQGAIATSKNEKWLFAVNAGSNSVSSFRITNSGNLNLASTISTSGIRPVSV
                     TLHNRILYVVNAGSDDISGFRIGNGGTLTAINGSIQSLSSSGSGAAQISFSPNGRYLY
                     VTEKGTNNISIFPVNNQGVAGAGTSTPSTGQTPFGFDIARDRYMIVTNAAGGAAGQSS
                     ATSYNGINSGNISAVNGAVGNNQAAACWAAVTKYGRFVYVTNTASNNISSYYIFPNGA
                     LFLINPDITAGNGPTEIIVADDNIFVYALNSASHTITSYRRSLLGNLVEISTTSGLPN
                     AATGIASVK"
BASE COUNT         2008 a         1165 c          957 g         2244 t
ORIGIN      
        1 catattgtta cataagtaaa gaaattttta ttacactgga gaaaattcag gtttttgtat
       61 ttagaattga atcataaaaa agcaaaaaat ctgcttaatt aatcttctcc tttcatatgt
      121 taaattttct tgttcaaaaa attttacgtt aatattttgt gggaacattt attacattaa
      181 caggggtaaa tgtaataaac agaaattact tgtatttatt taattcttaa aaatgtatta
      241 aaaaaatatc tgtaaaaata attattttaa ttttaaaaaa caaatcttac tgaccctcgt
      301 ttatcagttc aacaggatca tcgctgttcc agctaagata atatgaagga tcattaactt
      361 cttttgcaaa ttttgccagt tttagaggtc tgaaagtatc catcataact gctgtttcta
      421 aagtctcttt tttccctaaa tttgcttcta cagttccagg gtgtggacca tgtgggatac
      481 cgtttggatg cagacttatt gatgcaaagt caactccttt tctgcttgca aaatttccat
      541 ctacataata taacatctca tcagaatcaa gattactgtg attatatgga attggcactg
      601 ccaacggatg ataatccagc attctcggac aaaaagaaca aatcacaaaa cctggtgccg
      661 caaatgtctg atgaatcgga ggaggcatgt gaatttttcc tgtgatcggc atgaaatctt
      721 ctatattaaa tatccatgga tagtaataac cgtcccatcc gacaatgtca aaaggatgaa
      781 actcataatg aaaagttaca accttctctc ctttttttat cttcattctg aaatcacctt
      841 tttcatcaaa tgtaaccatc tcttcaggac atttaatgtc tctttcacaa tagggtgaat
      901 gttccattaa ctgaccatgt tcatttctgt atctgtgagg agtagaaaca ggtccacttg
      961 attcaaaaat tagatatctt gcatcttcag tttcatgaac taatttataa attacacctc
     1021 ttggaattac aatataatct ccttcccgaa atttcatata tcctagattt gactgtaatg
     1081 tcccttttcc ttcatggaca aatatcacct catcacataa tgcattttta taaaaataat
     1141 tcatttgatc cttgactctg catacactca tcactacatc attattaaac attacaggtt
     1201 ttctgcctga aaatgcatca ccttcatgat tcattggttt agtcctgaaa tgataaggtc
     1261 tcaaagaaga ttctttccac tcctcaattt ttacgatctc aacgtctggg gaaatatctt
     1321 taattttagc aggtgaattt atgtgatatg catttgtaga aatactgtca aatccaattg
     1381 tcgataaaag ttcttcttta tataaagatc cgtccggctg tctgaattgt gtgtgtcttt
     1441 tctgggggat ctggcctaac ttataataaa acggcatctg gtaagtttaa attttttctt
     1501 ttaaatttaa tttttatttt acgtttgact ttcggtaata cacaaaataa ataaattgag
     1561 ttccttcata aaaataaaat tacattgaac attgaacatt gaacattgaa caataataat
     1621 taatcattga tcattagctt tgctagtttc ttcaaactcg atcttctcaa gatatctttc
     1681 ggcatcaatg gcagacatac atcccgttcc cgcagcagta acagcctgtc tgtaatagtc
     1741 atcctgtaca tcaccgcatg caaaaacacc ttcgacatta gtttcagtgg ttgatttttt
     1801 tgtaataaga tatccgtatt cattcatatc gagaattcca ttaaagatct tagaatttgg
     1861 ttcatgacca attgctgtga agaatcctgc acattcaatt tccgaaacct cacctgttat
     1921 gacatttttt attcttgcac ctgttacaga ttttttattg tcttcagtct taccaaggat
     1981 ctcttctact acagtattta gaataaattt tattttcgga tttgcttttg ctctgtcaag
     2041 catgatcttt gatgctctga actcttcacg tctgtgaatt aagaacactt cacttgcatg
     2101 acgtgtcaga taatttgctt cttccattgc agtatctccg cctcctacaa caacgacttt
     2161 cattcctctg aaaaagaaac cgtcgcatgt ggcacatgag gagactccat agcccatata
     2221 ggtctgttca gattccagtc caatccattt tgcggatgcg cctgtagaaa taattacagt
     2281 gtcagtagta tattcattat tttcttcgtc agttatttta tgtggtcttg agctgaaatc
     2341 tactttagta atatttctat aaattgattt cgcaccaaat ctgtgagctt gctttctcat
     2401 aatgtccatg agttctggtc cctgaattcc atgttcaaat cctggataat tttctacttc
     2461 agtagtgatc gttagctggc cgcccggctg actgccttcg aatattatcg gatcaaggtt
     2521 agctctgcct gaatataatg ctgctgtcag ccctgctgga cctgaaccga taacaattac
     2581 tttgtgatga ttttgtgtag ttccgtttgt tgaaatttcc attagttgtt atttattaat
     2641 aaaatcggtt tactcaaaaa tctttatatc ggaaaatcta aaaaaaatta accgacttgt
     2701 gaagaataaa ttttgtcctt aagagtttca ttagaaggat ttatttctaa agacattttc
     2761 cagtatttta ctgctttatt actatcctta agtttgctgt acacatctcc catatgttca
     2821 agtaccactg cactggctgg atttacatcc aatgattttc cgataaactc ctcagctttt
     2881 ttatactttt tcattttaaa ataaatccaa ccaatagtat caagatatga tgcattgccc
     2941 ggttcttttt caacggctat ctttgacatg tataatgctt tttccagttc aacatctctt
     3001 tcggaaagat tataagcata attattaagt ataagtgcat tataaggatc gatctttaat
     3061 gcattctcat aggtttctac ggacttatca aatcgttttt gattatcata aattagtgca
     3121 agagaagaga gtgtatttat atctgcagga tttgcgtcaa gtgcagcttc ataataaggc
     3181 acggattcgg attcccttcc tgttccctgt aatgccagac cttttataaa atttagtctg
     3241 aaatcatccg gaaatttaga cagtccctga tcaattacag gcatagcttc atctgtcaat
     3301 ccttcctgat acagtgaaaa acctatattt ataaaaacgt ctcttgcagt gtctgccatt
     3361 tcaagagctg tctcaaagtt ttctttatat gccgattcat ttttatccaa cagatcaaaa
     3421 actccgagat aataatatgg aatccattca tctggatatt cactttttat accgataaat
     3481 atattttttg aaatgtcctt cgctgattca tcttgtgaaa ttaaattaaa ataaagttca
     3541 ccaacctgaa ctttctcaag aaaattcagt gaatcttttc caagtacctt actgaaattt
     3601 tcaaaggctt tgtcattctc attcagcttg aaatatattt tcacaagttc gttttgaact
     3661 tccttctcag tatgatctaa cgaaagtata ttttcataga ttactcttgc tttatcataa
     3721 tctttattat tcagataaag attagccagt atctttttta tgtttgcgtt aaacggatct
     3781 attttcagca tgctttcaag gacatccgat gcatttgcat aatccttatt tgcgatataa
     3841 atctcataca ttttatttaa cacatcaaaa tcatatccga tcttggctgt tattttttca
     3901 tatacagcaa gtgcatttgc aggcatgtta gtttcctgat atagtctcgc aagtgagtaa
     3961 agaccgtagg tatagtttga atctaatgaa atgatctgct catacgcagc aatggctttt
     4021 acaaattgtt tttttacaat atataaatta gcacttatct ctataaaatc ctgatcattt
     4081 ggttttaaat taagagcttt cttaatctcg gtcagagact catcaatttt attcatttta
     4141 aaatacacaa gtgcgagaga ataataaata ccgggtgatt tatcaaactc aagggcattt
     4201 ctgtaatttt ccacagctcc aatatagtcg gattttagtt ctaatgtttt tgcctctaca
     4261 aaataaaaca tggctttatc attatcactt actgatcctc ttttttgtga ttgagaaaat
     4321 aaatcacttt ctgaagtatt aagaaaggtg agaatacaaa aaaggagtat atactttaaa
     4381 ttaatattgg tcaaaatatg catttataca aaaataagat tattgaattg aacatacaat
     4441 tcagaataat taattaaccc gttttagatg cataaatgtt tcaaaaaaga ttttaataat
     4501 aaatttcgta gtattaaagc cgaattataa aataattagt agaaataaaa ccggttacaa
     4561 tttaagaact gaaacaactt catcacctac tttatcttca gttaatataa atccgagact
     4621 ttcgtaaact tttatagctt tatgattatc gggcttgtgt agaatgtcaa cagattctac
     4681 cttcaaatcc ctcaataaat ttaaaagctc actcaaagct tgctttccaa atccatttcc
     4741 ctgaaattta cggtctatca taagtctgtt aatatagtaa acaccgtcat caggatcttt
     4801 tgcatacatt aaaaatccga ccattgtctc atcattataa attgcaagag gattcatttc
     4861 tttatgagca taagcaagtg cgagtccatt aacatttgaa cctacgaatg taagctgatc
     4921 atcattgaca gtaagcgcaa tacactctct ccagttatcc tcatttattt ttcttaatgt
     4981 aatgttcatt taatttcaga attaatttat atagtcaacc attataataa ctatagaaat
     5041 aattttctat ggaaatatga ttatgaaatt ttaagaaata atatctgata aagtaattaa
     5101 actgaaatgg ttacaaaata cccggaacta agcccgggta ttaagaaaga aatgttttat
     5161 tgaaatttat tttacggatg caattccagt tgcagcattc ggtaatcctg aagttgtgct
     5221 tatctcaaca agatttccaa gtaaagatct tctgtaagaa gtaatcgtat gtgaagcaga
     5281 attcaaagca tatacaaaaa tattatcatc agccactata atttctgtag gaccattacc
     5341 tgcagtaatg tcaggattga ttaaaaataa agctccgttt gggaaaatat agtaggaaga
     5401 aatattatta cttgcagtat ttgtaacata aacaaatctt ccatacttag ttacagctgc
     5461 ccagcatgct gcagcctgat tatttccaac agctccattg acagcagata tatttcccga
     5521 atttattcca ttatatgaag tggcagaact ctgtccggca gctcctcctg cagcatttgt
     5581 cacgatcata tatcggtctc ttgcaatgtc aaatccgaac ggtgtctgac ctgttgaagg
     5641 agttgaagtc cctgcaccag cgactccctg attgttaaca ggaaatattg aaatattatt
     5701 agtccctttt tctgtaacat ataaatatct tccgttagga gaaaatgaga tctgagctgc
     5761 acctgatccg gatgaactca gactttgaat tgagccgtta attgctgtca gtgtaccgcc
     5821 gtttccaata cgaaatcccg atatatcatc acttccggca tttacaacat ataagatcct
     5881 gttatgcaaa gttacactta cgggtctgat accacttgtt gaaattgtag aagccaggtt
     5941 gagattacct gagtttgtga tcctgaaaga agaaacggaa ttacttccgg cgtttactgc
     6001 aaaaagccat ttctcatttt tactggtggc aattgctccc tgatttccta atccgcctcc
     6061 ggatccgatt ccacctgaag aaattgttga ctgaagaatt aatgaaccat tcaagttttg
     6121 tttgaataca agtatactgt tttgagtagc atcattgctt tgcaaataaa tataacctct
     6181 tggatttcta tggtcatttt cttcagaaag atccagctcg tcaggatttt taccttcttc
     6241 tgaaagatca ggcgcattac ttgacccggt agaatttggg ctaagcaata aagaatttga
     6301 atcttcattt gaacatgagg agaaaattac agcagaaaat aagattaaag ctgttaacat
     6361 catttttaag tttt
//
LOCUS       DAORVQ010000188         6338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7838_length_6338_cov_5.875060, whole genome shotgun sequence.
ACCESSION   DAORVQ010000188 DAORVQ010000000
VERSION     DAORVQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7838_length_6338_cov_5.875060"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..730
                     /locus_tag="PLX80_12175"
     CDS             62..730
                     /locus_tag="PLX80_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQY21590.1"
                     /translation="MILSAGYGIRLLPYTDKLPKALIEYKGKPMIEYQIERLKKIGIT
                     EIVINAHHFFDKIKEHFSKNDFGIKITVITERNILGTGGGIINAGKILKGEDYFMVTN
                     VDLDTDMDLERMVLYNLSTEPFATIAVQKRYTGRYLEFNNDREMLLKGRGNNKSHKFN
                     LFAFNGIHIISGRIFSEVINADSTDIIEIYLRAVNRGEVVRGYDVGDCKFKDLGKKEN
                     LFSD"
     gene            complement(731..967)
                     /locus_tag="PLX80_12180"
     CDS             complement(731..967)
                     /locus_tag="PLX80_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQY21591.1"
                     /translation="MSGLTEKNIEEVLSKIRPYLQKDGGDVELAGIREGGIVEVRLLG
                     SCKDCPLSPMTLRAGIERVLLKKIPEIKRIESVK"
     gene            complement(993..1421)
                     /locus_tag="PLX80_12185"
     CDS             complement(993..1421)
                     /locus_tag="PLX80_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HQY21592.1"
                     /translation="MRKYIYSVVIILLLIVSGCGSTGGSDSPANSLKDFVAALKEQNP
                     GKAWNFLSSNSQKMYDDIAKNRNQSGKEYFEKSVSNVSSLGLIGMDFEVIDEKKDGDN
                     AVIIIKSKDSTTSEYFSVKESGVWKLDYAKTIEENMKKVE"
     gene            complement(1450..2526)
                     /locus_tag="PLX80_12190"
     CDS             complement(1450..2526)
                     /locus_tag="PLX80_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQY21593.1"
                     /translation="MTEEKILEILSNVKDPLLKKDIVSLGFIKKISLNGKKLSLEIGS
                     SSSNNETTAQEIRNSFKENSPDIEIEDIKFTVGITEHINPKKSEIFPGVKNTVAVSSG
                     KGGVGKSTVAANLAVSMAKKGLKIGLIDADIYGPSIPLMFGINEKPKVQVVNGKNKLV
                     PIEKYGVKIMSIGFLMDTNSAVVWRGPMASSALKQFMSDVVWGELDILFFDMPPGTGD
                     IQLTLSQTVPLTGAVIVSTPQEISLADARKGYHMFEKVKVPTLGIIENMSFYLKTDGT
                     KEYIFGKEGGKKMSEEFNTKLLGEIPINTQIRISGDDGKPFVLNNPELTESEGFNKIS
                     DELINEINMINAEREESSDVVIEI"
     gene            2657..3268
                     /locus_tag="PLX80_12195"
     CDS             2657..3268
                     /locus_tag="PLX80_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21594.1"
                     /translation="MKLNKKAKTSLFLLITFGVIWLGGITVRFFIGNQLLDYDEFNFR
                     VSIPPDEENQIFKMISDASMMIMISYTIVLACSIVFMKTCKINLRENTWLLMCAMLFF
                     TFVPVEIFTSYLDMKFMMLFDKRPANHDELLKLFGERIGFLRGVPWIAVMSYFTIIWL
                     AVFQPMKKTALELEEDEVRLKETHGYKYIFHEEDDLAVSEYEQ"
     gene            3311..3643
                     /locus_tag="PLX80_12200"
     CDS             3311..3643
                     /locus_tag="PLX80_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21595.1"
                     /translation="MAKIIKKVQKKPLDENKESVIRELTEILEKIGFTVRTEKGTFKG
                     GFCILNEQKLFLLNKNLEQDRKISIIAKSISDFEIDDIYIKPGIREIIENEKSGENSG
                     ETADNKLF"
     gene            3663..4427
                     /locus_tag="PLX80_12205"
     CDS             3663..4427
                     /locus_tag="PLX80_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY21596.1"
                     /translation="MKVIILGTGTSQGVPVIGCKCETCLSDNPKDNRLRTSAYIKIND
                     SKLLIDTSVDFRMQMLGNKISDLDAVLYTHHHVDHIFGLDDLRQITQKHDKTIDIYGN
                     KNTLDEMRITFRYVFDKQLILHNSVPRVKFLVKANRKFTAAGIEIMPVEVHHGNIKIF
                     GYRINDFAYITDASAIDEKEMNKLKGLKVLVLNALRIRPHPTHFNLQEAVEISKMLKP
                     EKTYFTHLTHDILHDKINKTLPKGIELAYDGLELEM"
     gene            4442..5206
                     /locus_tag="PLX80_12210"
     CDS             4442..5206
                     /locus_tag="PLX80_12210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQY21597.1"
                     /translation="MKSTVINAAREAGKILKENFGGDFKIESKDVISNLVTEIDKLSE
                     KKIIEIIKKDHPGHYILSEEAGALIQDSEYKWLIDPIDGTVNYAHGIPITCVSIALEK
                     SGEIILGAVYNPISEEFFFSEKGEGAFLNDKKISVSKEIELGRSLLVTGFPYNSGTFK
                     PNPADTFKKFLMKDLPIRRLGSAAIDLCWTACGRFEAFWEYNLNAWDVAAGFLILSEA
                     GGRVTNFQGNEFSVYGKEILATNGFVHEEMMEVLNS"
     gene            complement(5492..5968)
                     /locus_tag="PLX80_12215"
     CDS             complement(5492..5968)
                     /locus_tag="PLX80_12215"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQY21598.1"
                     /translation="MQDKTIEYIKEAVRLSRENVNDGSGGPFGAVIVKDGKIIASGSN
                     KVTSKNDPTAHAEIEAIREACKILNTFNLTGCEIYASCEPCPMCLSAIYWARLDKIYF
                     ANTKKDAAEINFDDHFIYEEISKPHDKRKIPVVKIDHDDAKKVFDEWANKEGKIHY"
BASE COUNT         2046 a         1096 c         1069 g         2127 t
ORIGIN      
        1 ctattaacta ttaactatta actattaact attaactatt aactattaac tattgaaagc
       61 aatgatactc tcagccggtt acggcatccg gttattacct tatacagaca aacttcccaa
      121 agcacttatt gaatacaaag gaaaaccaat gatcgaatat cagatcgaaa gactgaagaa
      181 gattggtatt acggaaattg taattaatgc acatcatttt tttgataaaa taaaagaaca
      241 tttcagtaaa aatgattttg gaataaaaat cactgtcatt accgaaagaa atatacttgg
      301 cacgggggga ggtattatca atgcagggaa aattctgaaa ggtgaagatt atttcatggt
      361 aacgaatgtt gacttagata ctgacatgga tcttgaaaga atggtgcttt ataatttatc
      421 gacagaacca tttgcaacca tagctgtgca gaaaagatat acaggcaggt atctggaatt
      481 taacaacgac agggaaatgc ttttaaaagg caggggaaat aataaatcgc ataaatttaa
      541 tttgtttgct tttaacggga tacatattat ctcaggcaga attttcagtg aagtcataaa
      601 tgcagattca acggatataa ttgaaattta tctcagagct gtcaacagag gtgaagtggt
      661 cagaggctat gatgtgggtg attgtaaatt taaagatctg ggtaaaaagg aaaatctttt
      721 ttcagactga tcattttaca gattcgatcc ttttgatctc aggaattttt ttcagcagaa
      781 ctctctcaat accggctctc agtgtcatcg ggctgagcgg acaatctttg caggaaccta
      841 ataatctgac ttcaacgata ccgccttcac gtatccctgc caactctaca tcacctccgt
      901 ctttttgtaa ataaggtctg atcttagata atacttcttc gatatttttt tctgttagcc
      961 cgctcactat attaaatcca ttcagataat tcttattcca cctttttcat attctcttcg
     1021 attgtctttg catagtcaag tttccataca ccgctctcct ttacagaaaa atattctgaa
     1081 gttgtactat cttttgactt aattataatc acagcattgt caccatcttt tttttcatct
     1141 atcacttcaa agtccattcc gataagtccg agtgaactca catttgaaac acttttttca
     1201 aaatattctt taccgctctg atttctgttt ttggctatgt catcatacat cttctgagaa
     1261 ttggatgaaa gaaaattcca tgcctttccc ggattctgtt cttttaatgc tgctacaaaa
     1321 tcttttaaag aatttgcagg tgagtcagat ccgccggtac ttccgcagcc actaacgatt
     1381 aacagtaaaa ttattacgac tgaataaata tattttctca tttcattttt tttaatgtaa
     1441 aaatttttat cagatctcaa ttacaacatc tgaactttct tcacgttcag cgttgatcat
     1501 attgatctca tttattaatt catcacttat tttattgaaa ccttcagatt cagttaattc
     1561 aggattattt aagacaaaag gctttccgtc atcaccgctg atcctgatct gagtgtttat
     1621 aggtatttcg ccaagaagtt tcgtattgaa ttcttccgac atttttttcc ctccttcttt
     1681 gccaaaaata tattcctttg ttccgtccgt cttcagataa aaactcatat tctcgatgat
     1741 accgagtgta ggtactttga ccttttcaaa catatgatat ccttttctcg cgtctgccag
     1801 cgaaatctcc tgtggtgtgg aaacgatcac tgctcccgta agcggcactg tctggctcag
     1861 agtaagctga atgtcgcctg tacccggagg catatcaaaa aacaagatat caagttcacc
     1921 ccagaccaca tcactcatga actgtttaag tgcgcttgaa gccatcggtc cgcgccatac
     1981 tacagcggaa tttgtatcca taaggaatcc gattgacata attttgacac cgtatttttc
     2041 tatcggtacg agtttgtttt ttccgttgac cacctgaact tttggttttt catttattcc
     2101 aaacataagc ggaatagaag gtccataaat atccgcatct atcaatccga tctttagtcc
     2161 tttcttagcc attgatacag caaggttcgc ggctactgtc gactttccca ctccaccttt
     2221 tcccgatgat acagcaactg tattttttac tcccggaaat atttcggatt ttttcggatt
     2281 tatatgttcg gtgataccaa cagtaaattt aatatcctca atttctatgt caggtgaatt
     2341 ttctttaaaa gaatttctga tctcctgagc agtcgtctca ttattactgc tgctgcttcc
     2401 gatctcaagt gacaactttt ttccgttaag cgatatcttc tttataaatc caagcgatac
     2461 aatatctttc tttaataaag gatcctttac attagaaaga atttctaaaa tcttttcttc
     2521 ggtcattttc tagtttattt gagagggttt caagttacta ataataattt tctactgaaa
     2581 tgaaaatatg aaatttattt ttacatacat aacataaaat aaaattgcta aattacagat
     2641 aaatttttta gaaaaattga aattaaataa aaaagcaaag acttccttat tcttattaat
     2701 cacctttggt gtcatatggc tgggtggaat tacggtaaga ttctttatcg gaaatcaact
     2761 tttagattat gatgaattta atttcagagt gagtattcct cccgatgaag aaaatcagat
     2821 ttttaaaatg atatccgatg cctcaatgat gattatgatc tcatatacta ttgtacttgc
     2881 ttgttcaata gtatttatga aaacctgcaa gataaatctc agagaaaata cctggctcct
     2941 aatgtgcgca atgcttttct ttacgtttgt accggtggag atttttacgt catatcttga
     3001 catgaaattt atgatgcttt ttgacaaaag acctgcaaat catgatgagc ttttaaagct
     3061 gtttggagag aggattggat ttttaagagg cgtaccgtgg atagcagtga tgagttattt
     3121 tactattata tggcttgctg tatttcaacc aatgaaaaaa actgcacttg agcttgaaga
     3181 agatgaagtg agattaaagg aaactcatgg atataaatat atttttcacg aagaagatga
     3241 tcttgcagtc agtgagtatg agcaataaag gtcaatactt ttatgtaatt ttataaaatt
     3301 ttttctttca ttggcaaaaa taattaagaa agttcagaaa aaaccacttg acgaaaataa
     3361 ggaatctgtc atcagagaac taaccgaaat tctcgaaaaa atagggttta cagtaaggac
     3421 tgagaaaggc actttcaaag gaggcttttg tattcttaat gagcagaaac tttttctttt
     3481 gaataaaaat cttgagcagg acagaaaaat tagtatcatt gcaaaaagca tttcagattt
     3541 tgaaattgat gacatctata taaagccggg cattcgtgag attattgaaa atgaaaagtc
     3601 aggtgaaaat tcaggtgaaa ccgcagataa taaattgttt taatcctaca aaaatttaat
     3661 taatgaaagt aataattctc ggaacaggca cttcgcaggg agttcctgta attggctgta
     3721 aatgtgaaac ctgcctgtca gataatccaa aagacaatcg gctgcgaact tcggcataca
     3781 taaaaattaa tgattcaaaa cttttaatag atacttcagt ggatttcaga atgcagatgc
     3841 ttggtaataa aatttctgat cttgatgctg ttctttatac tcaccatcac gttgatcata
     3901 ttttcggatt agatgatctc agacagatca cacaaaagca cgataagaca attgatattt
     3961 acggcaataa aaacacactg gatgaaatga gaataacttt ccggtatgtg tttgacaaac
     4021 aattgatcct tcacaattca gtacctagag taaaatttct tgtcaaggca aacaggaagt
     4081 ttactgcagc aggtattgaa attatgccag tggaagttca tcatggcaat ataaaaattt
     4141 tcggatacag gataaatgat tttgcttaca ttacagacgc aagtgctata gatgaaaaag
     4201 aaatgaataa acttaaaggt ctgaaagttc ttgtgctgaa tgcacttcgt atccgtccgc
     4261 atccgactca ttttaatctg caggaagctg ttgagatttc gaagatgtta aagccggaaa
     4321 aaacatattt cactcatctc acgcatgaca ttcttcatga taaaataaat aagactttac
     4381 caaaaggtat tgagcttgca tatgacggac ttgaacttga aatgtaaaat aaactgataa
     4441 tatgaaatca actgtaatta atgctgcaag agaagcagga aagatactca aagaaaattt
     4501 cggaggtgat tttaagatag aaagtaaaga tgtgatttca aacctcgtca ctgagattga
     4561 taaactttca gaaaaaaaaa ttatcgagat cataaaaaaa gatcatccgg gtcactatat
     4621 actttctgaa gaagcgggag ctttaataca agattcagaa tacaaatggc tgatagatcc
     4681 gatcgacgga acagtgaatt atgctcacgg tattcctatc acatgtgttt ccattgcact
     4741 ggaaaaaagc ggagaaataa ttttaggagc agtatataat ccgatatcag aagaattctt
     4801 tttttctgaa aaaggagaag gagcttttct caatgataaa aaaatttcag tatcaaaaga
     4861 aattgaactc ggaagatctc tgcttgtaac aggatttcca tacaactcag gcacatttaa
     4921 acctaatcct gctgatactt ttaaaaaatt tctgatgaaa gatcttccca tcagacggct
     4981 tggttcggcg gcaatcgatc tatgctggac agcgtgcgga aggtttgaag ctttctggga
     5041 atacaatctg aatgcatggg atgtagctgc agggtttctg atactttcag aagccggagg
     5101 acgagttacg aattttcaag gtaatgaatt ctctgtatat ggcaaggaaa ttcttgcaac
     5161 aaacggattc gtacatgaag aaatgatgga agtattaaat tcataaattg acataaccag
     5221 taccaaaaga ttttaaaaca gtttttgttt ctcttattga ataatatttc tttcggcaac
     5281 aaccacttca ctccctcttt tttaaggggg aaatacattt attttattct gaattgtgct
     5341 gttaaaaatt cagacaaata tctcagtaag attcactgaa aattttctta acattttacg
     5401 aattccgtct ctgcagagtc acgtgctttt aggactctgt ggaaaaccaa aagttttaaa
     5461 ttcataaatt ctttttgatc cggatcattt tttaataatg gattttacct tctttatttg
     5521 cccactcatc aaaaactttt ttagcatcat catgatcgat ctttacgaca gggatttttc
     5581 ttttgtcatg cggttttgaa atttcctcat atatgaagtg gtcgtcaaaa tttatttccg
     5641 ctgcatcttt ttttgtattt gcgaaataga tcttatctaa tctcgcccag taaattgcag
     5701 acagacacat cgggcaaggc tcacagcttg cgtagatctc acagccggtg agattaaaag
     5761 tgtttagaat tttacatgct tctctgattg cttctatctc ggcatgcgct gtcggatcat
     5821 tttttgaagt taccttattt gatcctgagg caattatctt tccatctttg acaatcactg
     5881 ctccgaatgg accgccgctg ccgtcattta cattttcacg tgaaaggcgt acagcctctt
     5941 ttatgtattc tattgtttta tcctgcattt tttctaataa aaattttttc agttatgaac
     6001 ttataaatat ggctgtaaaa cttcaatgtt atttaaattc gaaagtgatt ttatttttta
     6061 ttgtaccgaa tattttatta tcgggtttaa aaaactaatt ataaaatcag ttgtggttta
     6121 aaataaattt tttagataat gaacagctaa gattttttca gtaagatatc agtgctgtcc
     6181 aaaatattta aattttaaat aaatagtttt gaaattattt aaaaaataca acttattaaa
     6241 ttaaatgaaa actatacctc ctttgaattt tctctattaa ctgatttatc accagcggcg
     6301 atttttttgg ttctttggag cctgccccct tgtgcttc
//
LOCUS       DAORVQ010000189         6317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7889_length_6317_cov_5.999521, whole genome shotgun sequence.
ACCESSION   DAORVQ010000189 DAORVQ010000000
VERSION     DAORVQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_6317_cov_5.999521"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PLX80_12220"
     CDS             <1..942
                     /locus_tag="PLX80_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21599.1"
                     /translation="SAKKFHLECNLVYGKSHGGVYLNNNNSDDLNINAEKIVTGEITE
                     LSLGTTAIISRGNGFMHINEILCRNLGHCLSHRAGEIIANIFKLITVTRRTGDSASTA
                     QISQGSQSQKLILYFDEINALSEGENISSNDGIQVTQGIGIFIGRKVTSKENYAVQFN
                     GFNLNGYIKCNEIESDLFYALGMNNFTTKVEFNINNIQGNNDGVVFAYGSANFILKNS
                     KIKNLNSNLPSRVISLYKYNNTFPNSTFNNIKLITGNTNTTEIILSAEFPFPNDPFIS
                     DITNFGLFVNEGINSNYINLLVGIDNNFLYIDSIDLN"
     gene            961..2250
                     /locus_tag="PLX80_12225"
     CDS             961..2250
                     /locus_tag="PLX80_12225"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21600.1"
                     /translation="MKKFLLILIFFTIFNKSNFVISQSIPTGWYIVSNLFNDSLLSVF
                     FSNNIKGWTVGKEGKIFISQNGGLNWSTQNSNSTSILENVFFLNNDTGFCIGSEGTII
                     KTTNGGINWIHQNSNSVNYLKSASFVNDSVGFVVGLDKTCLKTTNGGVNWNSVSFIDS
                     LDFYSVKFIDNNTGWISSMQYNSFPFQDSLVIFKSTDGGSNWYKQYIHPLEFSPFLSI
                     QFTDSLNGWMTIYRYDIDASSVYKTMDGGNTWNRYAVGNFGPYRIFFSNNRKGWGVGY
                     FNRISFTTNGGLTWINSPAFQVSLDYNSVFFIDSLIGWAVTGNGLVIKTTTGGILTDF
                     TNISSEIPIDFSLSQNYPNPFNPKTVISYELRITSDAELKVFDVLGNEVAELVNEKQN
                     AGSFSVEFDGSGFASGIYFYRLETGSFVDTKKMTLIK"
     gene            2583..2735
                     /locus_tag="PLX80_12230"
     CDS             2583..2735
                     /locus_tag="PLX80_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21601.1"
                     /translation="MERKIFIWRILYQIRIEPTAQKELKKLPRSDREKAARSTAESES
                     NPGPYG"
     gene            2882..4633
                     /locus_tag="PLX80_12235"
     CDS             2882..4633
                     /locus_tag="PLX80_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQY21602.1"
                     /translation="MKKKNIPTGDDISPEKKADQKKSVSRRNFIKLSAAAAASAGAGI
                     GMKKVFADNSDNSDTSDEFPKLLPVPDKPGFDHLVVLMFENRSFDNILGHLYEPGTVP
                     EGQTFNGLASGNYSNPSPDGPVNAHIYSGSTDIIMRSPSPDPGEEYPHVNTQLFGTVD
                     PPGNEHVDFKEMTKPFNAPPQGAAVKMEGFVKDYINNFAAVKGRQPSREEFEVVMGSF
                     SPEMMPVTSALARNFAVYDSWFCAVPSQTFCNRSFFNSSTSSGFVTNDGGPESYLKWE
                     KNTGQTIFNRLEEAGISWAVYFDESQVVSLTGFINIDALKPFWKTNFFGMKKYYEDAA
                     AGTLPAYSFIEPRLIFNHNDMHPPVASFKFKDEDGNEVEVGGEDDVRAGEKLLHDVYK
                     SIRTSASHKGSNALNTMLLITFDEHGGTYDHVPPPSAVPPGPVSDPEMGFTFDRLGVR
                     VPAIAVSAWTKSGTVINEEMHHAAVIATLCKKYKLKSLTKRDEDARDISNAINLNEPR
                     QPWDWPETTPQYIPPNPEAKGPFSKAVSQISLTPPALSLLGMLTEKFGLPEDVKPKTF
                     GEAYEILQKLGKGLFGV"
     gene            4908..>6317
                     /locus_tag="PLX80_12240"
     CDS             4908..>6317
                     /locus_tag="PLX80_12240"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21603.1"
                     /translation="MNNFIIKILYLSIILICVISHSSYSQQQQEWVRQFNSGSNQNDI
                     VTDMKIDNSGNIYMCGSIEYNSSLKDVLLLKYSSAGNLLWSRTWRGTGMNNTNESSAS
                     KILFDNEGFIYVLGNTIIDSNYLDFLLIKYKTNGDSIWVRTFNGTNGDVESERDIVID
                     PNNNIYITGLSYRFGSVDNETVKYDKNGDLKWSKKFNNFSGGNILLDSGFLYIGGASN
                     YNISILKYDTSGNSLDTFYCGTEKSDGAQLMVFDSQENLIVSGLENAGLPTQLNIVTA
                     KISQSGIKSWISVFNNNFDNRPEYLYDLKTDTSGNTLILVRSRNQQNNRGEIALIKYD
                     SNNGDSIWTRKYNLVPFSNEEPGKMAIDKDNNIYVIETSDSSVIFYRIVIIKYNSLGQ
                     LQWHKVYYPSLFSNSYGACISTDTLGNIYASGITNYAQTGGDDIVLIKYSVMTEVSEI
                     NTNLPEKFELYQNYPNPFNS"
BASE COUNT         2240 a          940 c         1159 g         1978 t
ORIGIN      
        1 tcagcaaaaa aatttcatct tgaatgcaat ttagtttatg gaaaatctca tggaggagta
       61 tatttaaata ataataattc agatgattta aatatcaatg cggaaaaaat tgtaacagga
      121 gaaattactg agctaagctt aggaacaaca gctataattt caagaggaaa tggcttcatg
      181 catattaatg aaatactttg cagaaatctt ggacattgtt tgtcacatag agcaggagaa
      241 ataatagcaa atatattcaa attaataact gttacaagaa gaaccgggga ttctgcatca
      301 actgcacaaa tctcacaagg ttcacaatct caaaagctca ttttatattt tgatgaaatc
      361 aatgctcttt cagaaggaga aaatataagt tcaaacgatg gcattcaggt tacacaagga
      421 attggaattt tcattggtag aaaagttaca tctaaagaaa attatgctgt tcagtttaat
      481 ggttttaatt taaatggata tatcaaatgt aatgaaattg aaagtgattt attttatgct
      541 ttaggaatga ataattttac tacaaaagtg gaatttaata ttaataatat tcaaggtaac
      601 aatgatgggg tagtatttgc atatggctct gcaaacttta ttctaaaaaa ttctaaaata
      661 aaaaacctaa acagtaatct cccatcaaga gtaatatctt tatataaata taacaatact
      721 tttccgaatt caacttttaa caatattaag ttaataactg gtaatacaaa tacaacggag
      781 ataattctat ctgcagaatt tccatttcct aacgatccct ttatatccga tattaccaat
      841 tttggacttt ttgtaaacga aggtattaat tccaattata taaatttact agtaggtata
      901 gataataatt tcttatatat tgattcaata gatcttaatt aatctaataa ttattatcaa
      961 atgaaaaagt ttttactaat attaattttt tttacaattt ttaataaatc aaattttgta
     1021 atttctcaaa gtattccaac aggttggtat attgtaagta atttatttaa tgattctttg
     1081 ttgtcagttt ttttctcaaa caatataaaa ggatggacag taggtaaaga aggaaaaatt
     1141 tttatatcac aaaatggcgg gttaaactgg agtactcaaa acagtaattc tactagcata
     1201 cttgaaaatg tgtttttttt aaataatgac acaggttttt gtattggcag tgaaggaaca
     1261 ataataaaaa cgacaaatgg aggaattaat tggattcatc aaaatagtaa ttcagtaaat
     1321 tatttaaaat ctgcatcttt tgtaaatgac tcggtcggat ttgttgtagg acttgataaa
     1381 acctgtttaa aaactacaaa cggaggtgta aactggaatt cagtttcatt tatagactct
     1441 cttgattttt attcagttaa atttatagac aataataccg gatggattag ttcaatgcag
     1501 tataattcat ttccttttca agattcattg gttatcttca aatcaactga tggtggcagt
     1561 aattggtata aacagtacat acatcctctt gaattttcac catttctatc aattcaattc
     1621 acagattctt tgaatggctg gatgactata tatagatatg atattgatgc aagtagtgta
     1681 tataaaacaa tggatggtgg aaacacatgg aatagatacg ctgttggaaa ctttggacca
     1741 tatcgaattt ttttttcaaa taatagaaaa ggttggggag tagggtattt taacagaatt
     1801 tcgtttacaa ctaacggagg attaacatgg attaattcgc ctgcatttca agtttctttg
     1861 gattacaatt ctgtattctt tattgattca cttataggat gggctgtaac cggtaatggt
     1921 ctggtaataa aaaccaccac cggcggtatt ctcacagatt tcacaaatat ctcctccgaa
     1981 attcccatag atttttcact ttcacaaaac tatccaaatc ccttcaaccc aaagacagtt
     2041 ataagttacg agttacgaat tacgagtgat gctgagctaa aagtatttga tgttctgggg
     2101 aatgaagtcg cagagcttgt aaatgaaaag cagaatgcag ggagtttttc tgtggagttt
     2161 gacggaagcg gttttgccag cgggatatat ttttacagat tggaaaccgg aagttttgtt
     2221 gatacgaaga aaatgactct gataaagtaa gagtaatttc aaattttaaa tgacgaagga
     2281 cgagttacga atatttttca ttaggtaaag tttttgtaat tcatacaaaa gtttattttg
     2341 atccaaaaga ttttcaaatt gacctgttta atttattttt tatttagaat atattattta
     2401 ctttagagat aaaaattatt atgagacaaa ctataatcaa agaaaaaaat ctaaatgtgt
     2461 tattttaacg gaggaagagt ttgatgcttt aaggaaagag ataatttatt attagaaaac
     2521 aactgaagaa ttacaggacg aactcgattc catagaagcg gaaaaaataa aaaagaatat
     2581 taatggaaag aaaaattttc atttggagga ttttatatca gataaggata gaaccaacag
     2641 ctcaaaaaga attaaaaaaa cttccccggt cagacaggga aaaagccgca aggtcaacag
     2701 ctgaatcaga aagtaatcca ggaccttacg gctgaaaaag ctaaaaggca ggaacaaata
     2761 ccgagtccga tccggagatc atagaattat ttatgaaata cattataaaa ttcccgttgt
     2821 tattattatc aaaattggat acagaaaaga tatttataga aattttacat gatatttaaa
     2881 aatgaagaaa aaaaatattc ccacaggaga tgacatatct cctgaaaaaa aagctgacca
     2941 aaagaaatct gtttcacgca ggaattttat aaaactttct gcagcggcag cagcatcagc
     3001 aggtgcaggt attggtatga agaaagtttt tgctgataat tcagacaatt cagatacatc
     3061 ggatgaattt ccgaaactac tgcctgtgcc ggataagccc ggattcgatc atttagtagt
     3121 gcttatgttt gagaacagat cttttgataa tattctcggg catttgtatg agccgggtac
     3181 agttccggaa ggtcagactt tcaacggact tgcaagtggt aattacagta acccttctcc
     3241 ggacggaccc gtgaatgctc acatatacag cggcagcacg gatattatta tgcggagtcc
     3301 ttcaccggat ccgggcgagg aatatccgca tgtaaacact cagctctttg gcacagtgga
     3361 tcccccggga aatgaacatg tagattttaa ggaaatgaca aaaccattta acgcgccgcc
     3421 tcagggagct gcagttaaaa tggaaggatt tgtaaaagat tatataaata attttgcagc
     3481 ggtaaaagga aggcagccgt caagagaaga gtttgaagtg gtaatgggat cattcagtcc
     3541 ggagatgatg ccggtgacaa gtgctttggc gcgaaatttt gctgtctatg attcctggtt
     3601 ctgtgctgtt ccgtcacaga ctttttgcaa cagatctttt tttaattcat ctacgtcgtc
     3661 cggatttgta acaaatgacg gaggtcctga aagttatctt aaatgggaga agaatacggg
     3721 tcagacaatt tttaaccgtc tggaagaagc gggtatttca tgggcagttt attttgatga
     3781 aagtcaggta gtatctctga ccggttttat taacatagat gcattgaaac cattctggaa
     3841 aacgaatttt tttggaatga aaaaatatta tgaagatgct gcagccggga cactgcctgc
     3901 ttattctttt attgagccaa gacttatatt caatcataac gacatgcatc cgccggtagc
     3961 ttcatttaag tttaaagatg aagacggaaa tgaagtggaa gttggcggtg aagatgatgt
     4021 tcgtgcagga gagaaacttc ttcatgatgt ctataaatca atccgtacaa gtgcatctca
     4081 taaaggttca aatgctttga acacaatgct tcttatcaca tttgacgaac acggcgggac
     4141 gtacgatcat gtacctccac catcggcagt acctccgggt ccggtatcag atcccgaaat
     4201 gggatttact tttgaccggc tcggagtgcg ggttcctgca attgcagtgt cagcctggac
     4261 aaaaagcggt actgttataa atgaagaaat gcatcatgct gccgttatcg caactctttg
     4321 taaaaaatat aaactgaagt cacttaccaa acgagatgaa gacgcacgag atatttcaaa
     4381 tgcaataaat ctgaatgaac cgcgtcagcc atgggactgg cccgaaacta ccccgcagta
     4441 tattccgcca aatcctgaag caaaaggtcc gttctcaaaa gcagtttcgc aaatctctct
     4501 tacaccgccg gcactttcac tgcttggaat gcttactgaa aaattcggat tacctgaaga
     4561 tgtaaaacct aaaacatttg gtgaagctta tgagattttg cagaaacttg gaaaaggttt
     4621 gtttggggtt tgaaggtatc gcctaaaaaa atcattctga atttcgcaat aacatcgagc
     4681 cgctgtcagt ttttaaaagt ttacgaatat ataagtattt tttaattttg aattgaatgt
     4741 atgatttagt atatgtagtg cacattgaaa aatctttaat taaaatatat gtattgtttc
     4801 ttaaaaactt ttcacttaca gtagcattac cccaaaggaa ttcaaattga aaaaagaaaa
     4861 atgaaacact taacatatat attactctct catttatttt atacctaatg aataatttta
     4921 ttataaaaat attatactta tctataattt taatttgtgt aatatcacat tcctcatatt
     4981 cccagcaaca gcaggaatgg gtaagacaat ttaattccgg atcaaatcag aatgacatcg
     5041 ttacagatat gaaaattgac aattctggaa atatctatat gtgcggttct atagaatata
     5101 attcaagtct aaaagatgtc ttgttactaa aatacagcag cgcaggaaat ttactgtgga
     5161 gcagaacgtg gagagggaca ggtatgaata atacaaatga aagtagtgcc tctaaaattc
     5221 tttttgacaa tgagggtttt atttatgtac ttggaaatac tattattgat tctaattatt
     5281 tggatttttt actgataaaa tataagacaa atggagatag tatttgggtt aggactttca
     5341 atgggacaaa tggagatgtt gagtcggaaa gagatatagt tattgatcca aataataaca
     5401 tttatataac gggcttatct taccgttttg gaagtgttga taatgaaact gtcaaatatg
     5461 acaaaaatgg tgatttaaaa tggtctaaga agtttaataa tttttcaggt ggaaatattt
     5521 tgctcgactc cggtttttta tatatcggag gagcttcaaa ttataatatt tcgatcctaa
     5581 aatatgatac atcaggaaat tcattagata ctttttattg tggtactgaa aaatcagacg
     5641 gagcacagct tatggttttt gactctcaag aaaatctaat tgtatcaggg ctcgagaatg
     5701 cagggttacc tacacagtta aatatagtga cagctaaaat atctcaatca ggaatcaaaa
     5761 gctggatcag tgtctttaac aataattttg ataatagacc tgaatacctt tatgatctaa
     5821 aaacagatac atccggaaat actttgatac ttgtaagaag cagaaatcaa caaaataata
     5881 gaggtgaaat tgctttaata aaatatgata gcaataatgg agattcaata tggactagaa
     5941 agtacaattt agtaccattc agcaatgaag aacctgggaa aatggcaatt gataaggata
     6001 ataatattta tgtaatagaa acaagcgact cgagtgttat tttttatcga atagtaataa
     6061 taaaatacaa ttcattggga caactgcaat ggcacaaggt ttactacccg tctctatttt
     6121 ctaactctta tggtgcttgc atctcaactg acactcttgg aaatatttat gcatccggca
     6181 tcacaaacta tgcccagaca ggaggagatg atattgtttt aataaaatat tccgtaatga
     6241 cagaggtttc ggaaataaac accaacttac cggaaaaatt tgaactttac caaaattatc
     6301 cgaatccgtt taattca
//
LOCUS       DAORVQ010000190         6280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_7990_length_6280_cov_5.091888, whole genome shotgun sequence.
ACCESSION   DAORVQ010000190 DAORVQ010000000
VERSION     DAORVQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7990_length_6280_cov_5.091888"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLX80_12245"
     CDS             complement(<1..324)
                     /locus_tag="PLX80_12245"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY21604.1"
                     /translation="MATENNTDIFIELLKPEYDNAIKYCKALCSKRSTDDAEDVLQQS
                     LLKAFENFDSLSDQSKFKSWFFKIITREFYNFVRKDFWKKFFPTDSVNSENPFPKVFS
                     AQDEDG"
     gene            641..1891
                     /gene="pepT"
                     /locus_tag="PLX80_12250"
     CDS             641..1891
                     /gene="pepT"
                     /locus_tag="PLX80_12250"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQY21605.1"
                     /translation="MNIMDINYTCTDRFLKYVKIDTQSSHDSDSFPSTKKQKDLGIIL
                     VEELKQMGISDAEIDKYGYVYATIPSNSDKNVPVICFCSHMDTSPDASGKNVNPVIHR
                     NYQGGDIILPKDKSKVILANEHPQLASQIGNDIITSDGTTLLGADDKAGIAEIMDAAN
                     YLMTHPDVKHGKIRILFTPDEEVGHGVDHVDIKKLGADFGYTMDGSDLGSVEDETFSG
                     DTVHLYIKGFNIHPGFSKDRMENAIKIANKIISRLPPDSLSPETTEGKEGFIHPVNIK
                     AEVDSAYIKFIVRDFDNAKLKEKEDLIQKIAEEVIKDYKKASFEIRIEESYRNMKEVL
                     DKNPEIMDYAVEAVNRTGVKAFRNSIRGGTDGCRLSFMGLPCPNIFAGGHAFHSNLEW
                     ISVQDMEKALETIIHLCMIWEEKA"
     gene            2204..3295
                     /locus_tag="PLX80_12255"
     CDS             2204..3295
                     /locus_tag="PLX80_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY21606.1"
                     /translation="MNENSFYIDSDISLAETMPAEFYTDDKYFEISKEKIFSKSWQFV
                     SDTESVKVPGQIFPFYMLENFLNTPLILTRDINDKLHCLSNVCTHRGNILVEGSSIEK
                     NLRCRYHGRRFGLDGKFISMPEFEEVKNFPSEKDDLPEIPFYVWDKFLFASLNPIAPL
                     HEFFGEMFSRISFLPVNEFLYDAPKSKDYLVKAHWALYCENYLEGFHIPYVHNSLNEK
                     LDYSGYTTELFRFSNLQIGHANKKGGEECFKIPEGHSDHGKNISAYYFWIFPNIMFNF
                     YPWGLSINIVKPIKKDLTKVSFISYVYDESKLNEGAGSALDKVEREDEAIVENVQRGI
                     NSGLYNRGRYSVNRETGTHHFHRLISEFL"
     gene            3292..3720
                     /locus_tag="PLX80_12260"
     CDS             3292..3720
                     /locus_tag="PLX80_12260"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQY21607.1"
                     /translation="MKLSFLFVGVQFFCISAIMLTGEIFPSDIIKMILLIITAVPGLI
                     AMYSFRFRFNIFAEIPESHKLNTSGIYKYVRHPMYTSVILMTLLWVWNDFSLMRFIIW
                     IILLIDMIFKLKLEERLLTVAYPEYEEYIKKTKMFIPYLY"
     gene            complement(3744..4781)
                     /locus_tag="PLX80_12265"
     CDS             complement(3744..4781)
                     /locus_tag="PLX80_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY21608.1"
                     /translation="MKVLFSNSHKLIIFTFIFLFSLYLISESSHPFLSCQNDQTLSIK
                     DSIVPWRAPEVSSIPDNEDGKIIILGRNIFNKTNLYLGPDVSNEANRYTNAGLACQNC
                     HLKGGTLKNVMGLVGVYYNYPKFDTRSGKNINIEERINNCMKRSLNGKPLPDTSKEMT
                     ALVSYMKWLSTYAPKGTKVEGQGLPKIELINRAADKENGKNVYLNYCTTCHADNGQGV
                     LRSPGNVDVLADSLKGYEFPPVMGSNSFNDGAGMYRLLTATSFIYAKMPYNDAILSLE
                     DSYDVAAYIISTPRPKLPGIESDYPDLKFKPVDFPFPPYEDKFSAEEHKYGPYQPMLK
                     EGETSKFVKPL"
     gene            4952..>6280
                     /locus_tag="PLX80_12270"
     CDS             4952..>6280
                     /locus_tag="PLX80_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21609.1"
                     /translation="MNKIFLLFLLVIFSNNTFSQTIDEKEIFSLDYKGNTELYNFIMD
                     EASGNFCYAYLIPDEKKTFLISPKGQSEKYDFIMTFDTKFDSKGNYFTNAGNYESDYG
                     IDNYFLIINGKTIKGLEYIESSSSFIDKKDEYVFIFKIKEDYFIGYAKADGSFRQSEK
                     YDIVKPVYKYFQGRGMEGDNEQIGDEYFYRNADGERGFIVVKNGKAGLMFGEDIKMTD
                     YSDIHESSLTLDNNNELTFIAKNGGKFYENAGDEFVVSGDIKYNSFDYVTPPVLFNKN
                     NEPVYKASDSTEQFTHNKYLVKNNEKLPLNFEQSEVDKKPVLIFDIYDIKFGENDDIT
                     YFAASDEIFPANPDDASSYDEYYVKTYYVKNDKAFPIGYSTGNIIYDKEGNMIYAGIS
                     DLKKKEYLLMQTNGESIIIIGQGDYDLIGDYGFMKNGELYYFGQQFADEKT"
BASE COUNT         2171 a          965 c         1071 g         2073 t
ORIGIN      
        1 tccgtcttcg tcttgtgcag agaaaacttt tggaaatggg ttttcggaat ttacgctatc
       61 agtcggaaaa aattttttcc agaaatcctt cctcacaaaa ttatagaatt ctcttgtgat
      121 tattttaaag aaccatgatt tgaacttgct ctgatctgaa agtgagtcaa aattctcaaa
      181 tgctttcagc aatgactgct gcaggacgtc ttctgcatca tctgttgacc gcttcgagca
      241 caatgcctta cagtacttta ttgcattgtc gtattccggc ttgagtaact caatgaatat
      301 gtctgtatta ttttctgttg ccaatttata taaacttata agttttgata attagacgtt
      361 ttgaattccc taaaagtcgc aaactcaatt ttaaattaca aattattaat tcccactcgt
      421 atgcatatac ctttcggtta tatgtatgaa attgaaaaac cgcttcatat acacctccgg
      481 caatttatct caagcggata tggatataaa acgattgaat aatattgaac ggatttcaaa
      541 aaattataca actttttttc tatgtctttt tcaatcataa attttaatat tcatgttaaa
      601 taaattttac cgaaaatttt aataaatcaa atactaacaa ttgaatatta tggatattaa
      661 ttatacatgt acagataggt ttttgaaata tgtaaaaatt gacacacagt catcacatga
      721 ttcggatagt ttcccatcaa caaaaaaaca aaaagatctg gggattatac tcgtagagga
      781 attaaaacaa atgggaatca gtgatgctga gatagataag tatggatatg tatatgcaac
      841 tataccatca aactccgata agaatgttcc ggtgatttgc ttttgttcac atatggatac
      901 ttctccagac gcatccggta aaaatgtaaa tcctgtcatt cacagaaatt atcagggcgg
      961 agatatcata ctgccaaaag ataaaagcaa agttattctt gccaatgaac atcctcagct
     1021 tgcatcacag atagggaatg atattattac ttctgacgga actactcttc tcggtgctga
     1081 tgacaaagcc ggaattgctg agatcatgga cgcagcaaat tatttaatga ctcatcctga
     1141 tgtaaagcat ggaaagatca gaattctttt tacgccggat gaagaagttg gtcatggagt
     1201 ggatcatgtc gatattaaaa agcttggtgc ggactttgga tatacaatgg acggatcaga
     1261 tctcgggagt gtggaagacg aaacattttc cggtgatact gttcatcttt atataaaagg
     1321 ttttaatatt catcccggat tttccaaaga cagaatggaa aatgcaatca agatagcaaa
     1381 caaaatcatc agcagactcc cacctgattc actttctcct gaaactactg aaggtaaaga
     1441 aggattcatt caccctgtca atattaaagc tgaagtcgat tccgcatata taaaatttat
     1501 tgtcagggat tttgataatg ctaagctgaa agaaaaagaa gatctgattc agaaaattgc
     1561 tgaagaagtt attaaagatt ataaaaaggc atcgtttgaa attcgaatag aagagtcgta
     1621 cagaaacatg aaagaagtcc tcgacaagaa tcccgagata atggattatg cagtcgaagc
     1681 tgtaaaccga acaggagtta aggcattcag aaattcaata cgaggcggaa cagacggatg
     1741 cagactttca tttatgggac ttccttgtcc gaatattttt gccggcggac atgcttttca
     1801 ttcaaatctt gaatggatct ccgttcagga tatggagaaa gctttagaaa ctataattca
     1861 tctttgtatg atttgggaag aaaaggctta agagcaattg aaaattgcaa attgcaaatt
     1921 acgaattacg aaatacgagt tacgagttgc gaattactaa ttacgaatca cgagttataa
     1981 aaacaaatta caaattgttt tattacaacc taaatttatt gccaattatt aaaaaaatta
     2041 tctgtgaatt ttcttgagat atatttttaa attattagta tcactattca gaataattta
     2101 aaaatatttc cccattaaaa aaaacgggga gcgaaggggg ttgttgccaa gagaaattac
     2161 aaattacaaa tattaaatta caataatttt tttaaaagtt attatgaatg aaaatagttt
     2221 ttatatcgat tcggacatat cacttgctga gacaatgcct gctgagtttt atacggatga
     2281 taaatatttt gaaatttcta aagagaagat attttcaaag agctggcagt ttgtaagtga
     2341 tactgagtcg gtcaaagtac ccggccagat attccctttt tatatgcttg aaaattttct
     2401 caatactcca ctcattctta caagagatat aaatgacaaa ctgcactgtc tttcaaatgt
     2461 ctgtacgcac agaggaaata ttctcgtcga aggaagctct attgaaaaaa atttaagatg
     2521 cagatatcat ggcagacgat tcggtctcga tggtaaattc atttcaatgc ctgaatttga
     2581 agaagtaaaa aactttccat ccgaaaaaga tgatttgccc gaaattcctt tctatgtctg
     2641 ggataaattt ctttttgcat ctttaaatcc aatagctcca ctgcatgaat tctttggtga
     2701 gatgttttcc agaatttcat ttctccctgt aaatgaattc ttgtatgatg ccccgaagtc
     2761 aaaagattat ctcgtgaaag ctcactgggc tttgtattgt gagaattatc ttgagggatt
     2821 tcatataccg tatgttcata attccttaaa tgaaaaactg gattactcag gatatacaac
     2881 tgagctgttc agattttcaa atcttcagat tggacatgca aataaaaagg gaggcgaaga
     2941 atgtttcaaa attcctgaag gacattctga tcatggaaaa aatatttcag catattactt
     3001 ttggatattt cctaatatca tgttcaattt ttatccgtgg ggattatcaa taaatattgt
     3061 aaaaccaata aaaaaagatc tgacaaaagt ttcgtttata tcttatgtct atgatgaatc
     3121 aaaattaaat gaaggtgcgg gaagtgcatt ggataaagtc gagcgggaag acgaagcaat
     3181 tgtcgagaat gttcagagag gaatcaattc aggtttgtat aacagaggaa gatattctgt
     3241 aaacagagaa actgggacgc atcattttca cagactgatc tcagagtttt tatgaaactt
     3301 tcatttttat ttgttggagt tcagtttttt tgtatatcag cgataatgct gacaggtgag
     3361 atttttcctt ctgatattat caaaatgatt ttgctgataa tcactgctgt cccgggtttg
     3421 attgctatgt attctttcag attcagattt aatatatttg ccgaaattcc tgaatctcat
     3481 aaacttaata cttcaggaat ttacaaatac gtcagacatc caatgtacac ttcagttatt
     3541 ttaatgacat tactttgggt ctggaatgat ttcagtttaa tgagatttat tatctggatt
     3601 atacttctga tcgatatgat ctttaaactt aaacttgaag agagacttct tacagtagct
     3661 tacccggaat atgaagagta tataaaaaaa actaaaatgt ttattcctta cttgtattga
     3721 gcagcaaaga tcaataatct atgctacagc ggctttacaa atttacttgt ctcaccttct
     3781 ttcagcatcg gctgatatgg accatactta tgctcctctg ctgaaaattt atcctcataa
     3841 ggtggaaacg gaaaatctac tggtttgaat tttaggtccg gatagtcaga ttcaattccg
     3901 ggaagctttg gtcttggagt tgagattata taagctgcta catcatagga atcttcaagt
     3961 gacaaaattg catcattgta aggcatcttt gcataaataa aagaagttgc agttaaaagt
     4021 ctgtacattc ctgcaccgtc attaaaactg ttcgatccca tcaccggcgg gaattcataa
     4081 ccttttaaag agtcagccaa cacatccaca tttcccggac ttcttaaaac tccctgacca
     4141 ttatctgcgt gacaggtcgt gcaataattc agataaacat tctttccatt ttctttatca
     4201 gcagctctgt taataagttc aatttttgga agtccctgtc cttctacttt tgtacctttc
     4261 ggtgcataag tactcagcca tttcatatat gaaactaaag ctgtcatttc ttttgaagta
     4321 tcaggcaaag gttttccgtt caaacttctt ttcatacaat tgttgatcct ctcctctata
     4381 tttatattct tccctgatct tgtatcaaat tttggataat tataatatac accaaccagt
     4441 cccattacat ttttcaatgt accgcctttt aagtgacaat tctggcaagc caaacctgca
     4501 ttcgtatatc tgtttgcttc attgctgaca tcaggaccta aatataaatt agtcttatta
     4561 aaaatatttc tgccaagtat tataattttt ccatcctcat tgtctggaat agagcttact
     4621 tcaggagctc tccatggaac tatggaatct tttatactta gtgtctgatc attttggcag
     4681 gaaagaaacg gatgtgaact ttcggaaata agataaagtg aaaataaaaa aataaaagta
     4741 aagatgatta atttatggga attactgaat aaaactttca tatgtcattt tttttaaaat
     4801 ttaatataaa ttccaataaa ttgcaatttc taattaagga taggttaatt taaaaattgt
     4861 ccaaacatta attctataat ctggaaaaac atattacaac taacttttta attaaacttg
     4921 gaattaataa attaccaaaa aaaacaactg aatgaataaa atttttttac tttttttact
     4981 tgtaatattt tcgaacaata ccttctcaca gactatagat gaaaaagaaa tattcagtct
     5041 ggactataaa ggaaatacag aactttataa ttttattatg gatgaagctt caggaaattt
     5101 ctgttacgct tatcttatac ctgatgagaa aaaaacattt cttatctctc ccaaaggtca
     5161 atcagaaaag tatgatttta ttatgacttt tgatacaaag tttgattcta aaggaaatta
     5221 ttttactaat gccggtaatt acgaaagtga ttatggaatc gataattatt ttttaattat
     5281 taacggaaaa actatcaaag gtcttgaata cattgaatca tccagtagtt ttatcgataa
     5341 aaaagatgaa tatgtattca tctttaaaat taaggaagat tattttattg gctatgcaaa
     5401 agccgatgga agttttagac aatctgagaa atatgatatt gtcaaaccag tctataaata
     5461 ttttcagggg agaggtatgg aaggtgataa tgaacaaata ggagatgaat atttttacag
     5521 gaatgctgat ggtgaaagag gatttattgt tgttaaaaat ggtaaagccg gtttaatgtt
     5581 tggagaagat attaaaatga cagactattc cgatattcat gagtcaagtc tcacattaga
     5641 taacaataat gaacttactt ttatagcaaa gaacggtgga aaattttatg aaaatgcagg
     5701 tgatgaattt gtagtatcag gtgatataaa gtataatagt tttgattatg ttactccgcc
     5761 tgttctcttc aataaaaata atgaaccggt atataaagct tcagattcta ctgaacagtt
     5821 tactcataat aaatatctgg taaaaaataa tgaaaaactc ccattgaatt ttgaacaatc
     5881 tgaagtagat aaaaaaccgg tacttatttt tgatatatat gatattaaat ttggagaaaa
     5941 cgatgacatc acttattttg ctgcttccga tgaaatattt cctgcaaacc ctgatgatgc
     6001 atcctcatat gatgagtatt atgtaaaaac atattatgtt aaaaacgata aagcatttcc
     6061 aatcggatat agcacaggga atattatcta tgataaagaa ggaaatatga tctacgcagg
     6121 aatttctgat cttaagaaaa aagaatattt gttgatgcaa actaacggag aaagtattat
     6181 tattataggt cagggagatt atgatctgat aggggattat gggtttatga aaaatggaga
     6241 gctgtattat tttggtcagc agtttgcaga tgaaaaaacc
//
LOCUS       DAORVQ010000191         6267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8019_length_6267_cov_5.321797, whole genome shotgun sequence.
ACCESSION   DAORVQ010000191 DAORVQ010000000
VERSION     DAORVQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_6267_cov_5.321797"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLX80_12275"
     CDS             complement(<1..449)
                     /locus_tag="PLX80_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21610.1"
                     /translation="MKIILNLFLLLIAFTNLFSQNASTYFPANPGYKWFYKNTPLDSI
                     NNPINSQSTFQVDSFATNVNYNGLPASLVLSQSGLSNVNQQGLFTDSVHYNFQSTNGY
                     YYLNPLSFLTSIPIFDSVAFIDFLRSFEAWYSYYRFSQNVNTNYTIFS"
     gene            771..1619
                     /locus_tag="PLX80_12280"
     CDS             771..1619
                     /locus_tag="PLX80_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY21611.1"
                     /translation="MKIGDYTIHPVQTGLFKLDGGAMFGVVPKNLWQKTNPSDEQNRI
                     DMCTRALLLDNGKKKILVDTGIGYKLSEKVNKIYDVDYSEYTLEKSLTDIGLSKSDIT
                     DVILTHLHFDHAGGNTEYNENNEPVPSFPNAVYHVQKKQYEWALNPSERDRASFFPEN
                     YKPLEKAKVLNLTDGEQKFDEFISLIPVNGHTNNMQMIKISDEKNSMLYLADLIPTAG
                     HIPAPYIMGYDLFPLTTLEEKHKYLKEITENEMMIFFEHDPHNMAGKIGKNERGYFLK
                     EKIEIE"
     gene            2062..3552
                     /locus_tag="PLX80_12285"
     CDS             2062..3552
                     /locus_tag="PLX80_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQY21612.1"
                     /translation="MINFSPVDYTIIIVYLIAVAIFGIITSGKQTSVKDYFLGSRNIP
                     WWAACFSVVATETSTLTFISIPGLAYISNMNFLQLSIGFLIGRIIVAMVFIPRYYSGN
                     METAYEFLGNRFGEPMRKYASVTFILLRIFADGVRLFTTAIPIKFITGLGYFECILIV
                     GVITLIYSYIGGIKAVIWTDVIQMFVYTFGAVASMVIIYNLLPNGWDDVTAFANPGDK
                     FQIFNLKMSDSFSSFFTGGYGLIGGLVGGAFLAMASHGTDQMIVQRLLTCKDKRSSQK
                     AVIVSGIIVTVQFTIFLVVGLMLYALYEGVDFKSLVINGQNLTKSDEIFPMFIVQSLP
                     VGLAGIVVAGLLSASMSTLSSTFNSLASTTILDLFKGKKFIKDEKDELKYSKLATIFW
                     AIVIMGTGLLFQSETNPAVDFALKIQSLIYGGLLGVFLLGIMSVKAQLKDAVISYTFA
                     IVILSLLFILPKFGVMPAINLTWFTLFGVVITFVIANITMLFRKRI"
     gene            3626..6151
                     /gene="priA"
                     /locus_tag="PLX80_12290"
     CDS             3626..6151
                     /gene="priA"
                     /locus_tag="PLX80_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQY21613.1"
                     /translation="MSELFANVAFNLPLDNLFTYKIPPHLISSAIPGKRVLAGFGNKK
                     ITGIIVSVIDNTEHQKLRTILDLIDSNVIIKDELMEFCKWISEYYCAPIGEVIFSALP
                     GRMNISSDKFYKLHEKYLKELDDSELKEEIYLKIIEALEDKEFATKKQLERKLNFKDL
                     SSYIKILENKKIIYSENQFSKPVSEKKIRTARLNFSLKEIDKIIIQHKIRSDKQIAFL
                     KLLSEDRFNSIREAAELCGISSSSVNSLLLKELIEIKEEREFRQSEDIFREDDKTLTL
                     NPDQEKILKGISEYTDKEIFKVFLIYGVTGSGKTEVYINLIKRVLKKKKTAIVLVPEI
                     SLTPQLIYRFRSAFGNIIGVIHSKLSEGERRDTFDRILNGDVKIVIGARSAVFAPLKN
                     IGIIVVDEEHDSSYKQENSPKYNGRDCAIYRGLLNNAPVVLGSATPSLESYYNGQTGK
                     YALFSLPLRASNIKMPDIRIIDLLKKEKDEDNEYDNSDYKFKKKDFFDTIDKVKIRFL
                     SKDLIYEIGERLEKKESIIILQNRRGYHSYLECLSCGEVEMCIRCNIALTFHKAIDLQ
                     KCHYCGFSKKKNRVCSSCGSDKLIEKGAGTERVEEELEKIFPRAIIKRIDSDSLNSKR
                     QFHTLLKDFYDKKIDILAGTQIISKGLDFPDVTLVGVVNADIGLLNPDFRATEKTFQI
                     LTQVSGRSGRSEKAGEVLIQTNHSEFYVFENVKNHDYINFYEKEIAVRRQLNYPPFSR
                     LVIIESKSEDKNLCESKIKELYNLVISMDKEKLLEVLPPNPPLFSKLKDKYRFHLIIK
                     SIKEKDISGAFLKWILNEVKYYIKSNIPKKVMITIDVDAVNLM"
BASE COUNT         2197 a          926 c         1214 g         1930 t
ORIGIN      
        1 gagaatatcg tataatttgt atttacattt tgtgagaatc tgtaataact ataccaagct
       61 tcaaaagatc ttaaaaaatc aatgaatgca actgaatcaa aaattggtat tgaagtaaga
      121 aaactcagcg gattcagata ataatatccg ttcgtagatt gaaaattata atgtacagaa
      181 tctgtgaaaa gtccctgctg attaacattt gaaagtcctg actgtgataa taccaaagac
      241 gcaggtaatc cgttgtaatt cacattagtt gcaaatgaat caacctgaaa agtcgattga
      301 gaatttattg gattatttat tgagtcaagc ggtgtgtttt tataaaacca tttatatccc
      361 ggattagcag ggaagtaagt agatgcgttt tgggagaata aattcgtaaa cgcaattaat
      421 aggagaaata aatttaatat aattttcata agtctatttt tttttggatt aagttatata
      481 catttattgt taaattaaat tcaaatgtat taattgtata gtttaaaata ttttgattga
      541 ttgagcatta aattacatta gctgtcgttt tgacaacccc accccggccc tccccttgcc
      601 aaggggaggg agtttatgat taagttttaa gtctttgagt cttcgagcct ttgaggcaaa
      661 gctttgtgcc tttgtgattt cgtggtaaca ccaaatgacg gaaatacaat tttattttac
      721 atataaaatt aaaatcataa tctttataat gactgaaatt aattttaaaa atgaaaatag
      781 gggattatac tattcatcct gtacaaacgg gattgtttaa gcttgacgga ggagcaatgt
      841 ttggcgtagt gccaaagaat ctctggcaga aaacaaatcc ttctgatgaa cagaacagga
      901 tcgatatgtg cacgagagca ttacttctgg acaatggtaa gaaaaaaatt cttgtcgata
      961 cagggatcgg atataaactt tcagaaaaag taaataagat ctatgatgtg gattattcag
     1021 aatatactct tgagaaaagt ctgacagata tcgggctgag taaaagtgat atcactgatg
     1081 taatactgac tcatcttcat ttcgatcatg caggcggaaa tactgaatat aatgaaaaca
     1141 acgaacctgt cccttcattt ccaaatgcag tttatcatgt acaaaagaag cagtatgaat
     1201 gggctctgaa cccatctgag agagaccgcg caagcttctt tccggaaaat tataaaccat
     1261 tagaaaaggc taaagttttg aatttgacag acggtgagca gaagtttgat gaattcattt
     1321 cactgatccc tgtgaacgga catacaaata atatgcagat gattaaaatt tctgatgaaa
     1381 aaaattccat gctttatctt gctgatctga ttccaactgc cgggcacatt cctgctcctt
     1441 atataatggg atatgatctt tttccgctga ctacacttga agaaaaacat aaatatctga
     1501 aagaaataac tgagaatgaa atgatgatat tctttgaaca tgatccgcat aatatggcag
     1561 gtaaaattgg taagaatgag agagggtatt ttttaaaaga gaagattgag attgaataaa
     1621 tttatagaag atatttgtat aaaactttta gtattatatt agatatttat agtttcagct
     1681 gttctttttt gagcgatcaa aaaagaacca aaaaaatcgc cgctggtgat aaattgccgg
     1741 aaattctttc gcatggctac aaaaatataa actcctcacc tgttagaaaa acactaacag
     1801 gttcgtcaaa cagtatattt ttgcttaacg ccatagctca gaatttctct tcggcaattt
     1861 atcacaggcg gattatcaaa ataagttaat agtcaaaatt caaatggagt tagtatttaa
     1921 tttgatttga ttgataaaca acatattcta aaatatttta aactcttttt taatgattga
     1981 attttttcgg actaaaaagt ttctaattag ataatattta aagatactaa gcaaataaat
     2041 taaataataa attataacca cttgataaat ttttccccag ttgattatac aataataatt
     2101 gtctacctga tcgccgttgc aatattcgga atcataacct cgggtaagca gacttctgta
     2161 aaagattatt tccttgggtc tcgaaatatt ccctggtggg cggcatgttt ctctgtagtt
     2221 gcaactgaga cgagcacgct gactttcatc agtatccccg ggcttgcata tatttccaac
     2281 atgaattttc ttcagctttc cataggattt ctgataggga gaataatcgt ggcaatggtt
     2341 ttcattccaa gatattattc aggaaatatg gagacagcat atgagttcct gggaaacaga
     2401 ttcggcgaac cgatgagaaa atatgcttcg gtaactttta tactattgag aatttttgct
     2461 gacggagtga gattatttac aacagctatt ccgataaaat tcatcacagg acttggatat
     2521 tttgaatgta ttctaattgt cggagtaatc actttgatct attcatatat cggcgggatc
     2581 aaagctgtga tatggactga tgtgattcag atgtttgtat atacattcgg agctgtagca
     2641 tctatggtta taatttataa tttgttgccg aacggatggg atgatgtcac agcttttgct
     2701 aatccgggtg ataagtttca gatattcaat cttaagatgt cggacagttt ctcatcattc
     2761 ttcacaggcg gatatggact gatcggcgga cttgtcggcg gcgcatttct ggcaatggct
     2821 tcacacggta ctgatcagat gatcgtgcaa aggctgctga cctgtaaaga taaaagatct
     2881 tcacagaaag cagtgatagt gagcggaata attgtaactg ttcaattcac aatattttta
     2941 gtagttggat taatgctgta tgcattatat gaaggtgtcg attttaaaag tcttgttata
     3001 aatggtcaga atcttacaaa gtcagatgaa atctttccaa tgttcattgt acagagtctg
     3061 ccggtcggac tggcggggat tgtagtcgca ggattactgt cagcttcgat gtcaacattg
     3121 tcatcgacat ttaactcact tgcatccact actatacttg atcttttcaa gggaaagaaa
     3181 tttataaaag atgagaagga tgaattaaaa tattcaaaac tggcgactat attttgggcg
     3241 atagtgataa tgggaacagg acttttattt cagagtgaaa caaatcccgc agttgatttt
     3301 gcacttaaga ttcagtcgct gatatacgga ggactgctcg gagtattcct gcttggcata
     3361 atgagcgtta aagcacaatt aaaagacgct gtcatttcat atacctttgc gattgtaatt
     3421 ctctcgctgc tgttcattct gccaaaattt ggagtaatgc cggcaataaa tcttacatgg
     3481 tttacattat ttggagtagt gatcactttt gtaattgcaa atattacgat gctgtttaga
     3541 aagcgtatat gatcaggctg caaataatgt aaatacaaaa ctgaaaacga aagaacatat
     3601 ttattattaa ttttattcaa agtaagtgag tgaacttttt gccaatgtcg ctttcaatct
     3661 gcctttagat aatcttttca cttacaagat ccctcctcat ttaatttcat cagcaatacc
     3721 gggtaaaaga gtacttgctg gttttggaaa taaaaagatc acaggaatta tcgttagtgt
     3781 gatagataat acggagcatc aaaaattacg aacaatacta gacctgatcg acagcaatgt
     3841 aattatcaaa gatgaactga tggaattctg caagtggatt tcggaatatt attgcgctcc
     3901 aataggtgaa gtaatttttt cagcattacc gggcaggatg aatataagtt cagataaatt
     3961 ttataaactg catgagaaat atctgaaaga gcttgatgat tcagaactga aagaagaaat
     4021 ttatttgaag atcatagaag ctcttgagga taaagagttt gcaacaaaaa agcagttaga
     4081 gagaaagctg aatttcaaag acttatcttc ttatataaag atattagaaa ataagaaaat
     4141 catatattct gaaaatcaat tctcaaagcc tgtcagtgaa aagaaaatta ggactgcccg
     4201 tctgaatttc agcttaaagg aaattgataa aataattata cagcataaga tacgctccga
     4261 taaacagatt gcatttttaa aacttctctc tgaggacaga tttaattcta taagagaagc
     4321 agcagaatta tgcgggatca gttcatcttc tgtaaattct ttattgctaa aagaacttat
     4381 tgaaataaaa gaggaacgtg aattccgaca gtcagaggat attttcagag aagatgataa
     4441 gacgctgact ctaaatcctg atcaggaaaa aattctaaaa ggaatatctg aatatacaga
     4501 taaggaaatt tttaaagtat ttctcatcta tggagttacg ggcagcggaa aaactgaagt
     4561 atacatcaat cttatcaaaa gagtcttaaa gaaaaagaag acagcgatag tgctggttcc
     4621 ggaaatttca cttacaccac agctgatcta cagattcaga agtgcatttg gaaatattat
     4681 cggagtcatt cacagtaaac tttccgaagg cgaaaggcgg gatacatttg acaggatatt
     4741 aaacggtgat gtgaaaattg tgatcggagc caggtctgct gtctttgcac ctttgaaaaa
     4801 tatcggaatc atagtagtag atgaagagca tgactcatct tataagcagg aaaattctcc
     4861 gaagtataat ggcagggact gcgcaattta cagaggtttg cttaacaatg caccggtagt
     4921 actcggatcg gctacacctt cactggaatc atattataac gggcagacag gaaaatacgc
     4981 tttgttcagt ttgccgctaa gagcttcaaa tattaaaatg ccggatataa gaattattga
     5041 tcttttaaaa aaagaaaagg atgaggataa tgaatatgat aacagtgatt ataaatttaa
     5101 gaaaaaagat ttttttgaca cgattgataa agtaaagatc agatttcttt caaaagatct
     5161 gatatatgaa ataggggagc ggcttgagaa aaaagaaagt ataataattt tacaaaacag
     5221 aaggggatat cattcttatc tggaatgtct tagctgcggt gaagtggaga tgtgtatcag
     5281 atgtaatatt gcactgacat ttcacaaagc catcgatctt cagaaatgtc attactgcgg
     5341 atttagtaaa aagaagaaca gagtctgttc atcatgcgga tccgacaagc tcattgagaa
     5401 aggtgcgggg actgaaagag tagaggaaga gctggagaaa atttttccac gcgcaattat
     5461 aaagagaatt gattctgatt ctctgaattc aaagagacag ttccatacat tgttaaaaga
     5521 tttttatgat aagaaaatag atatacttgc aggaacacag atcatttcca aaggtctgga
     5581 ctttccggat gtgacactgg tcggagtagt gaatgctgac atcggacttt tgaatccgga
     5641 cttccgtgca acagaaaaaa cttttcagat acttacacag gtgtcaggga ggagcggcag
     5701 aagcgagaaa gcgggcgaag ttttaataca gacaaatcat tcagaatttt atgtatttga
     5761 gaatgtgaag aatcacgatt acataaattt ttatgaaaag gaaattgcag tgagaagaca
     5821 acttaattat ccgcctttca gcagacttgt aattattgaa tcaaaatccg aagataagaa
     5881 tctttgcgaa agtaagatca aggaattgta taatctggtc atttcaatgg acaaagaaaa
     5941 attattagaa gtacttcctc ctaatcctcc tctgttttca aaattaaaag ataaatacag
     6001 atttcatctc attataaaat caattaaaga aaaagatata tcgggagcat ttctgaaatg
     6061 gatattgaat gaagtgaaat attatataaa gagtaacatt cctaaaaaag taatgatcac
     6121 gattgatgtg gatgcggtaa acttaatgta gactggaagt taatagttag tagttaatag
     6181 ttaatagtta atagttaata gttaataatt attagttgtt gattttttaa atgaactaaa
     6241 aatttaattt actaactact aactact
//
LOCUS       DAORVQ010000192         6267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8023_length_6267_cov_3.915969, whole genome shotgun sequence.
ACCESSION   DAORVQ010000192 DAORVQ010000000
VERSION     DAORVQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8023_length_6267_cov_3.915969"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..392)
                     /gene="ssrA"
                     /locus_tag="PLX80_12295"
     tmRNA           complement(26..392)
                     /gene="ssrA"
                     /locus_tag="PLX80_12295"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(452..802)
                     /locus_tag="PLX80_12300"
     CDS             complement(452..802)
                     /locus_tag="PLX80_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21614.1"
                     /translation="MAKSKQQNLQTIRSKKQKREQSHLHFAMTSKNYTIITAGIVIII
                     LGYLLMNENSVSGFLPTVIAPVLLVFGYCVVIPIGILYKGKEINETEEVIKDNIKAET
                     ESNVSAATSNVKTV"
     gene            complement(808..2184)
                     /locus_tag="PLX80_12305"
     CDS             complement(808..2184)
                     /locus_tag="PLX80_12305"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQY21615.1"
                     /translation="MEKIEKYIESNKDNYVEELKEFLRIPSISTNPENKADVRNCAEY
                     VRDQLNLIGLENVKIYETPGHPIVYGDWLYAGEDKPTILIYGHYDVQPVDPLNLWTDP
                     PFEPTVRNGNIYARGSADDKGQVFIHMKSIQAHLLNNGSLPVNIKFLIEGEEEVGSIH
                     LDEFILKNKELLKCDNVVVSDSAMFDYDVPSICYGLRGLAYMQVDLTGPNRDLHSGSF
                     GGAVDNPINALAHIIVQLKDNKGKILIDGFYDDVLPISEKERVEFKKLPFDEKKYMEG
                     LEINEVFGEEGYSTLERASARPTLDCNGIWGGFQGEGAKTVLPSKASAKISMRLVPNQ
                     DPEKIEKLFSDFVHKITPATVKVKVTSLHGGKGAITPIDSPAIDAAVEALRKGFGKVP
                     VFTREGGSIPIVNTFQTMLEADTVLLGFGLPDENAHSPDEHLNLSNFQRGILSVAHFF
                     TELSKIKK"
     gene            complement(2257..3693)
                     /locus_tag="PLX80_12310"
     CDS             complement(2257..3693)
                     /locus_tag="PLX80_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21616.1"
                     /translation="MNNILKKSLLFLFLLALSSNLYSKDNFDEGIKAAQRGDYVNAVK
                     LLKSTVNTENNYEGYYYYGLALMNTGSTKEAEKYLNKALNEDDEGIGALIAMGDLYSM
                     KKDYTKADSYYKKALKAEPEAVNVLLAQAKNYNRAGKVDDAITALTLATTISKENPNV
                     YVGLGDSYYYRRSYKPAIDNYNQALKLDKNSAAALFGLGAVAFKEKNYNEALEFYQKS
                     IDANPNFADAYLEKGRLLYFNENYDAAMKAFEKYASLKPGSVEGETYIAKTLYGMKKY
                     DEAEAKLEEIIKNNPDEDVSSAYKYLAYVNHDKENYDQAIDYFKKVPDDFFEVEDLIM
                     LAKINSSKGDYTTAYTNMNKAIQMDSADDNTYYQLGIIQFNEKKYEASILSFNKAIEM
                     GSTQLAAYVYKGLCYYSLQKYDEALKTFNEANALDNSYVQSWLWKARSEVALNDFDAA
                     RLSYAKVLELEPENTSAKEDLELLDKKQ"
     gene            complement(3733..4695)
                     /locus_tag="PLX80_12315"
     CDS             complement(3733..4695)
                     /locus_tag="PLX80_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQY21617.1"
                     /translation="MNRLYIYITIVICVFTIQSCDLDEIKSVATSGEMKIATDENVEP
                     LMKDEVREFQRLNPEAKIVMSSGPTNNVITELLNRDTKFIVSTRVLNQEEKDIAVKNK
                     MEITEIPFAIDAIGFIVNLKNPVTRVTSDDLRKILSGETKNWLDITAQDEEQNKEAKS
                     FFNNSNEKIKLYIQRKNSSTHDYLLDSILKQSQYSENAVICSTTVQILESVRDNENAI
                     GIVNMNWLSTGNHDTIDSTVKPLRVSKIWDNGKQDDYSEFHQGLIFNGKYPYRRTIYI
                     ITSEVGITLATGFITFLTKTDGQKIVLKNSLVPVNQPIRTIQIN"
     gene            complement(4692..4937)
                     /locus_tag="PLX80_12320"
     CDS             complement(4692..4937)
                     /locus_tag="PLX80_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21618.1"
                     /translation="MNAGIYINFATSFFTFIFGILLLTGIVFPGGSGNTKLMLGIVLM
                     IYGVYRFINAYAKLKQNKIKENISKLEHDRNKLLEGK"
     gene            complement(5111..5974)
                     /locus_tag="PLX80_12325"
     CDS             complement(5111..5974)
                     /locus_tag="PLX80_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQY21619.1"
                     /translation="MSSHENIDNSEVVFEEINKKMKYGAPELKKFYQKYVTRGLIIAV
                     ALHGFFIGGYALAMYYEKVKANEELEGQERIVNLSDLDVPPPIDEVPPEPPKDIALKD
                     LSALTPEPVAKKEVTEEVKLKSQKELEDVKLPVASEGTDDPNLVNPNAVFTGKVQEKK
                     VEEKVEKKEEKKKDTFQQFEVEKAPVAVNLGSVKGSMRYPEIARQSGMEGRVVAKVLV
                     GTNGSVIKVGGISGPEVFRDEVADKVMELTFTPALQNGQPVKCWVSVPFSFTLSAKDK
                     KKADEDVEEDE"
BASE COUNT         1925 a         1145 c          853 g         2344 t
ORIGIN      
        1 caaaagatta aataaagtac tattttagtg gaaatgccga gagtcgaact cgggtccgaa
       61 aagaagacca ttaaaacctc tacatatata gtctgtactt taattttcac aacactcagg
      121 cagacagaca agccaaagta attgctattc tgattaagtt ttgcccgtat aactcagacg
      181 atcatacgga ctatctcatc tgatttgaca cttcagaaac tccggtgaga gagaatctct
      241 aaagcgaggt tgctttaaat taagcagcca ttgcgtagtt atattctacg ttattgttgt
      301 tgtttattgt ttttctacct ttttaaagtg atggcaggaa cactaaatgc agttctaaac
      361 atctttcttc ccgtcgatac cgttgcattc ccgaaatttc aaagaacaaa aaaacttttg
      421 atctgctttg caaattaata tgaaaagtga atcagactgt tttcacattt gaagttgctg
      481 ctgatacatt actttcagtt tctgctttaa tattatcctt tattacttct tcagtttcat
      541 taatttcttt tcctttatac aaaattccta taggtattac tacgcaataa ccaaaaacaa
      601 gtaagaccgg tgctattaca gtaggcagga atcctgaaac tgaattttca ttcataagta
      661 aatatcccag aataattatg actataccag cggttataat tgtataattt tttgatgtca
      721 ttgcaaaatg aagatgactt tgttctcttt tctgcttttt cgatctgata gtctgtaaat
      781 tttgctgttt tgattttgcc attaaaatta ttttttaatt tttgataatt ctgtaaaaaa
      841 atgtgcgaca cttaaaattc ctctttgaaa attacttaag tttaaatgtt catccggcga
      901 atgagcattt tcgtcaggta aaccaaatcc aagtaacaca gtatcagctt ctaacatagt
      961 ctgaaatgta ttaacgatag gaatagatcc accttccctt gtaaagaccg gtactttacc
     1021 aaatcctttt ctgagtgctt ccactgctgc atctattgca ggagaatcga taggagtgat
     1081 agctcctttc ccgccatgga gagaagttac ttttactttt actgttgccg gagttatttt
     1141 atgtacaaaa tctgaaaata acttttctat tttttcagga tcctgattag gtactaacct
     1201 catagaaatt tttgcactcg cttttgaagg aagtactgtc tttgcacctt caccctgaaa
     1261 accaccccag ataccattac agtcaagtgt aggccgggct gaagctcttt ccaaagtaga
     1321 atatccttcc tcaccaaata cttcatttat ctcaagacct tccatatatt tcttttcatc
     1381 aaagggtaat tttttaaatt ctactcgttc tttttctgat atcggaagta catcatcata
     1441 aaatccatct atcaatattt tacccttatt atctttgagt tgtacaataa tgtgtgcaag
     1501 agcatttata ggattatcta cagcaccacc gaatgaacct gaatgaaggt ctctgttagg
     1561 tccggttaga tcgacctgca tgtatgcaag tcctctgagt ccatagcaaa ttgaaggaac
     1621 atcgtaatcg aacattgcac tatcagagac gacaacatta tcacatttca aaagctcctt
     1681 atttttaaga ataaattcat ctagatgaat acttccgact tcttcttcac cttcgattaa
     1741 aaactttata tttaccggaa gtgaaccatt attgagaaga tgtgcctgaa tgcttttcat
     1801 gtgaataaaa acctgacctt tatcatcagc cgaaccacgt gcatagatat ttccatttct
     1861 tactgttggt tcaaatggcg gatcagtcca taaattcagc ggatctaccg gttgaacgtc
     1921 ataatgcccg taaatcagaa ttgtcggttt atcttcacct gcatataacc agtctccata
     1981 aactattgga tgtccgggag tttcataaat ttttacattt tcgagtccta ttaaatttaa
     2041 ctgatctcta acatattcag cacaatttct aacgtctgct ttattttcag gattagtact
     2101 aatggaagga attcttaaaa attcttttaa ttcttcaacg taattgtctt tattactttc
     2161 tatatatttt tctatttttt ccattaagtt atttaataaa aatgcggtca aaaagaccgc
     2221 acaaatttaa ctaatttaat tcaaacttta aatttactat tgtttcttat ctaataactc
     2281 caggtcttct tttgcactgg tgttttcagg ttcaagttca agtacttttg cataacttaa
     2341 tcttgctgca tcaaaatcat ttaatgcaac ttctgacctt gctttccaaa gccatgactg
     2401 cacataagaa ttatccagag catttgcctc attgaaagtt tttaatgctt catcatattt
     2461 ctgaagtgaa taataacaaa gtcctttata tacgtaagca gcaagctgtg tagaacccat
     2521 ttcaattgct ttattaaaac ttaaaatgga agcctcatat tttttttcgt tgaattgaat
     2581 tattccaagt tgataataag tgttatcatc tgcactatcc atttgaattg ccttattcat
     2641 attagtatat gcagtagtat aatctccttt actggaatta atttttgcaa gcattatcag
     2701 atcttcaact tcaaaaaaat catccggtac tttcttaaaa taatcaatcg cctgatcata
     2761 attttcttta tcgtgattta catatgctaa atatttataa gctgatgaca catcttcatc
     2821 aggattattt tttataatct cttctaattt agcttcagcc tcatcatatt ttttcatacc
     2881 atataaagtc tttgcaatat atgtttcacc ttcgactgaa ccaggcttta aggatgcata
     2941 tttctcaaat gctttcatag ctgcatcata attttcatta aaataaagca gtctaccttt
     3001 ttcaagatag gcgtcagcaa aattaggatt tgcatctata gacttttgat aaaattccag
     3061 agcttcattg taattttttt ccttaaaggc gacagctccc aatccgaata aagcagcagc
     3121 actattttta tctaatttca atgcctgatt gtaattatca atagcaggtt tgtaagatct
     3181 tctgtaataa taagaatcac ctaatcctac atatacattt ggattttctt ttgaaatagt
     3241 agtagcaaga gttaatgcag taatagcatc atctacttta cctgcacggt tatagttttt
     3301 tgcctgtgca agtaatacat ttacagcttc aggttcagct tttaaagcct ttttataata
     3361 gctgtccgct tttgtataat cctttttcat actgtaaaga tcacccatag caataagagc
     3421 tccaattcct tcatcatctt catttagagc tttgttaaga tatttttctg cttcttttgt
     3481 agaacctgta ttcataagtg caagtccgta ataatagtaa ccttcataat tgttttcagt
     3541 atttacagta ctctttagca atttaacagc attgacataa tcccctctct gtgcagcttt
     3601 tataccttcg tcaaaattat cttttgaata tagatttgaa gataaagcta acaaaaataa
     3661 aaacagcaga gattttttta aaatattgtt cattttatta tttattaatt aattaatttt
     3721 tttattttaa cttcaattta tttgaattgt tctgataggt tgattaacag gaaccagact
     3781 gtttttcaat acaattttct gaccatctgt ttttgttaaa aaagtaatga atccagtagc
     3841 caaagtaata ccaacttcag aagtaattat ataaatagtc cttctgtaag gatatttacc
     3901 gttaaaaata agtccctgat gaaattcgga ataatcatcc tgtttaccat tatcccagat
     3961 cttggaaact ctaagaggtt ttacagttga atcaattgta tcatgatttc cagttgagag
     4021 ccagttcata tttacaattc caattgcgtt ttcattatct ctgacagatt ccagaatctg
     4081 aactgtagtt gaacatatta cagcattttc tgaatattga gattgcttta aaatactgtc
     4141 gagtaaataa tcatgtgtag aagaattttt tctctgaata tataatttaa ttttttcatt
     4201 cgaattatta aaaaaagatt ttgcttcttt attctgttct tcatcctgag cagtaatatc
     4261 aagccagttt ttagtttcac cacttaatat ttttctgaga tcatcagatg ttacccttgt
     4321 taccggattt tttaggttga ctataaatcc tattgcatcg atagcaaaag gaatttctgt
     4381 aatttccatt ttatttttta cagcaatatc tttctcttcc tgatttaaaa ctcttgtact
     4441 tacaataaat ttagtatccc tatttaacag ttcggtgatc acgttattag taggtcccga
     4501 tgacataacg atttttgctt caggattcaa tctctgaaac tccctgactt catctttcat
     4561 aagaggttcc acattttcgt cggtagcaat tttcatttca cctgaagttg ctacagattt
     4621 aatttcatct agatcacaac tctgtatagt aaatacacat atgacaattg taatatatat
     4681 ataaagtcta ttcatttacc ttcaagtagt ttatttctgt catgttcaag tttcgaaata
     4741 ttttctttga ttttattttg tttaagtttg gcataagcat ttataaatct atatactcca
     4801 taaatcataa gaactatacc aagcattaac tttgtattac cgctgcctcc aggaaacact
     4861 attcctgtca gaagtaaaat tccgaatata aaagtaaaaa aagatgttgc aaaattgatg
     4921 taaattcctg cattcatttg gttataatta aaataagcct gaggtatatt tcaggctttt
     4981 aaaattttaa tacagtttat aattatttat tgctgttttg gaaagtcaaa ttgactgata
     5041 aaaaaacttc tgctttaatt gaatttagaa atttttaata gtcctgaaat ctataaaaaa
     5101 tcagttaaaa ttattcatct tcttctacat cttcatcagc ttttttctta tctttagcgc
     5161 taagagtaaa actaaaaggt acacttaccc agcattttac aggttgacca ttttgaagag
     5221 caggagtaaa agtaagttcc attactttat cagcaacttc atctctgaat acttcaggtc
     5281 cgctgatacc accaaccttg atcacactac catttgtacc tacaagaact ttagctacca
     5341 ctcttccttc cattccagat tgtcttgcaa tttcaggata tctcatagat ccttttactg
     5401 atcctaaatt tacagcaaca ggagcttttt caacttcaaa ctgctggaaa gtatcttttt
     5461 tcttttcttc ttttttttct actttttctt caactttttt ctcttgtact tttccggtaa
     5521 acacagcatt cggatttaca agattaggat catcagtacc ttctgatgct acagggagtt
     5581 ttacatcctc cagctctttc tgacttttaa gttttacttc ttcggtaact tctttttttg
     5641 ctactggctc aggtgttaaa gcagaaagat cttttaatgc aatatctttt ggtggctctg
     5701 gtggaacttc atcaattgga ggtggaacat caaggtcaga aagatttaca attctttcct
     5761 gtccttcaag ctcctcattc gcttttactt tttcataata cattgcaaga gcatatccac
     5821 caataaaaaa accgtgaagt gccaccgcaa taatcaaacc tcttgttaca tatttctggt
     5881 aaaatttttt caactcaggt gctccgtatt tcattttttt attgatctct tcgaagacaa
     5941 cttcgctgtt atctatgttt tcatggctgg acatatgacc ccctgctttt aaagttatga
     6001 aatttcattt gctaactgtg aatttaaaat tttgttccgg aatttgctaa acttaaaata
     6061 ttttttatac aataaaaatg ttaaatttaa taaaattttt aaattatctt tttggaccaa
     6121 aataaggtaa tactccaatt ggattattaa aatatttttt tataagatct tttgaagaat
     6181 ctacttctgg atttttaact tcctctgaac caacctcaac tatttcatct tctataattt
     6241 cagtatcttc tgtttgcaaa atacttt
//
LOCUS       DAORVQ010000193         6183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8209_length_6183_cov_4.986619, whole genome shotgun sequence.
ACCESSION   DAORVQ010000193 DAORVQ010000000
VERSION     DAORVQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8209_length_6183_cov_4.986619"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3397
                     /locus_tag="PLX80_12330"
     CDS             <1..3397
                     /locus_tag="PLX80_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21620.1"
                     /translation="KGIGLRGSSNIFVVRGRFNQISNLANTRIDGFGITAHEYGHIEF
                     TWDHTNSARYDIMNAFDVHFKDCPQLPNPVFRLKEGWLNPIAMENTQQIQSLEPIETS
                     YQCGVITIYGKPNAAPDWSSGECYIVENRRRIGFDRKIAESFPDLLGGLLIWHYSPYK
                     NHPDPVCSAGFDKSVKFISAVGNPDLCGSRGDPQDFFAYAPNIPPGSVDYYNIDSART
                     HSGDDVKTGIELRNIRQINYSDLNSSIKFDLNYLISEPKVYDYIVYQREYVARKVTLS
                     NDVFYHEKDISEFYKILPGTVIDVGSQMRVKNIEARGETNNMITFKGPSFSNSSGNYI
                     SNYNKGFHLNLLTGFQDIDSCYLENINIQNMATGIPEITCNQIYPFPISLKNIIVTSN
                     FTDKNDYDLDIFGSYISELDLSNMQISFRILTKFTENVSINSSRVSFYGNNFFAHDKN
                     YLYTNSKIDNYHENINPPAFFYELTTGEGWQGIRMDGGGIEFRHHTNNSLKIKNANIG
                     IDITDPTETVIIKNMTFENNKDYDISLYKTKNENGEIPVISQNIFSQNNQNPKIANII
                     LSECEQVSIDTNNFNNTGYFGIILMNSQNPIINYNTITGGNLNGTSSSGIFSYGTNGF
                     IKCNTITQCMNYGVLLDNSQPVLFNNIIAANGLGLYLSNNSNPIMAPAYLLDQTYVLG
                     GYNYIHHNGSEEIYCNNSKYNYSTPLMYKGFNSIVDDSPNLNDILIYNKEVSGAVPFI
                     DCPYNYWGGGSPSGRLFPDYSFNYADYLTSQFSYACDVISDPAENSSEPNEYLLLGNV
                     LKNQYLNNFENSINYSNQLLSLQNSEINSIISLNNLFTSNIQTGGNFNDLRNYYLNQI
                     NLNPEDTIKNNKLLKLSIESKIVMNELESAVLEYDEILNNNLDEEIRFYTSIERSRAI
                     RLLLDSLLSFYQGDNISQDLKHLYENAINVKSNPNFMNQYNNLHNEDSRNIESNKTNK
                     SKNSIKAVNNFNALRNNINERISNLDNLNDRDKRNLIVEKNILDLAISNQLDNIASIK
                     DKLQYLNQKEIVVPLTYKLSQNYPNPFNPTTKINFSIPKDGKVVLKIYDVSGKEVYTL
                     VNEFKQAGNYNAIFNGSNLSSGVYFYRLNAGDFVESRRMILLK"
     gene            3588..3815
                     /locus_tag="PLX80_12335"
     CDS             3588..3815
                     /locus_tag="PLX80_12335"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQY21621.1"
                     /translation="MNYIALIKKYGNGYSAYIPDVPGCVSAAGTKKETLKLLKEELII
                     HLHDCSDNTSEETGSKSVRLSKDVKIITNPS"
     gene            complement(3799..4230)
                     /locus_tag="PLX80_12340"
     CDS             complement(3799..4230)
                     /locus_tag="PLX80_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21622.1"
                     /translation="MKTNFTKGDYLISSDKSLLNIKVIQKYLSHSYWAKNRLLKTTKK
                     SIGNSVCFGLYYKKTQVGFARVITDKATFAYLADVFILEEHRGKGLSKWMMKVILNYG
                     DLKNLRRWFLATKDAHSLYEKFGFHSLKEPEKLMEMFRKDL"
     gene            4314..5003
                     /locus_tag="PLX80_12345"
     CDS             4314..5003
                     /locus_tag="PLX80_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY21623.1"
                     /translation="MISTIKAVLISIHCVIIAILTIIVSPIDRRGRITHYLSKVFGGV
                     ILFIAGVKVTVSGTENLNKDETYIFISNHLSYFDIPILMKAIPNNVRFIYKDSLTKIP
                     ILGWGMYLGGYIPISRENIRDAMKSLKAAAVKIINGISVVIFPEGTRSYDGIPGEFKR
                     GMFVLAEESKVPLVPTTITGSEKIMPRGKFEIRSGDVHVNFSSPVDYRKDKELLDEIR
                     NIIVEQLKIKN"
     gene            5016..5306
                     /gene="gatC"
                     /locus_tag="PLX80_12350"
     CDS             5016..5306
                     /gene="gatC"
                     /locus_tag="PLX80_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQY21624.1"
                     /translation="MPVTIKDVEKIAKLAKLKFSESETGKLQTELNQILEYIDQLNEL
                     DLEGIEPLENINETSNVLRKDDSEKWLTTEEALKNAPSKTGKFFKVPKVLDK"
     gene            5303..6037
                     /gene="kdsB"
                     /locus_tag="PLX80_12355"
     CDS             5303..6037
                     /gene="kdsB"
                     /locus_tag="PLX80_12355"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQY21625.1"
                     /translation="MIIAGVIPARYASTRFPGKPLVMIDGKSMIQRVYEQSQKCRSVS
                     KVLVATDDKRIFDHVRSFGGEAVMTSVKHRSGTDRINEAVKNIKCDIVVNIQGDEPFI
                     HPGNIDKAIEPFLKDKKLNVSTLAIKFKDLSEIKDPNVVKVIFDINNYAIYFSRGILP
                     YDKDLISSVNERSTTKKYYKHIGLYVYRKSFLSEFVKMKKSYLEETEKLEQLRILENG
                     EKIKVILTNKNSTAVDTPEDLLLIKK"
BASE COUNT         2355 a          829 c         1018 g         1981 t
ORIGIN      
        1 taaaggcata ggtcttagag gtagttcaaa tatatttgtt gtaagaggaa gatttaatca
       61 aatatctaat ttagctaata ccagaataga tggatttggt attacagctc atgaatatgg
      121 gcatatagaa tttacatggg atcatacaaa ttctgccaga tatgatatta tgaatgcttt
      181 tgatgttcat tttaaagatt gtcctcaatt gcctaatcct gttttcagat taaaagaggg
      241 ttggcttaat cctattgcaa tggaaaatac tcagcagatt caaagtttag aaccaattga
      301 aacatcttat caatgcggag ttattactat ttatggaaaa ccaaatgcag cacctgactg
      361 gagttcgggc gaatgttaca ttgttgagaa cagaagaaga ataggatttg atagaaaaat
      421 tgcagaatca ttcccagatt tattaggagg attacttatc tggcactact ctccatataa
      481 aaaccatcct gatcccgtat gtagtgccgg atttgacaaa tctgttaaat ttatttcagc
      541 agtaggtaat ccggatctat gtggaagcag aggagatccg caggatttct ttgcatatgc
      601 tcctaatatt ccaccaggct ctgtagatta ttataatata gattcagcta gaacacattc
      661 cggtgatgac gttaaaacag ggattgagct tagaaatatt aggcaaatta actacagtga
      721 tttaaatagt tctattaaat ttgatttaaa ttatttgata tcggaaccaa aagtatatga
      781 ttacattgtc tatcaaagag aatatgttgc aagaaaagta actttgtcta atgatgtttt
      841 ttatcacgaa aaggatataa gtgaatttta taaaattctt ccgggtactg ttatagatgt
      901 tggcagtcaa atgagagtaa aaaatattga agcaagaggt gagacaaata atatgataac
      961 ttttaaagga cctagttttt ctaatagttc aggtaactat atatccaatt ataataaagg
     1021 atttcattta aatcttttaa ccggatttca agatattgac agttgttatc tggaaaatat
     1081 taatattcaa aatatggcaa caggaattcc tgaaattacc tgtaatcaaa tttacccgtt
     1141 ccctattagt ttaaaaaata ttattgtaac ttcaaatttc actgataaaa atgattatga
     1201 cttagatata tttggaagtt acattagtga attagattta tcaaatatgc agatttcatt
     1261 cagaattttg acaaaattca cagaaaatgt atccattaat tcatcccgag tttcttttta
     1321 cggcaataat ttttttgcac acgataaaaa ttatttatat actaattcga agattgacaa
     1381 ttatcatgag aatataaacc cccctgcatt tttttatgaa ttaactactg gagaaggctg
     1441 gcagggaatt aggatggatg gtggaggaat tgaatttaga catcatacaa ataattcatt
     1501 aaagattaaa aatgctaata ttggaattga cataaccgat ccaacagaaa ctgtcataat
     1561 aaaaaatatg acatttgaaa ataacaaaga ttatgatatt tcattatata aaacaaaaaa
     1621 tgaaaatggt gaaatacctg ttattagtca aaatatattt tctcaaaata atcaaaatcc
     1681 aaaaattgca aatataatat taagtgaatg tgaacaggtt agtattgata caaataattt
     1741 taataataca ggatattttg gaatcatatt gatgaacagt caaaatccaa taattaatta
     1801 taatacaata acaggaggga atttaaatgg gacttcatca agcggaatat tcagttacgg
     1861 aacaaatggt tttataaaat gtaatactat tactcaatgt atgaactatg gagttctatt
     1921 ggataattca caaccggtat tatttaacaa tattattgct gcaaacgggt tggggttgta
     1981 tttatcaaat aactcaaatc caattatggc tcctgcttat cttttagatc aaacatatgt
     2041 attaggaggt tataattata tacatcataa tggcagtgag gaaatatatt gtaataattc
     2101 gaaatataat tacagtacac cattgatgta taaagggttc aattcaatag tcgatgatag
     2161 tccaaattta aatgatatat taatttacaa taaagaagta tcaggagcag taccttttat
     2221 tgattgtcca tataattatt ggggaggagg aagtccgtca ggaaggttat tccctgatta
     2281 ttcatttaat tatgcagatt atctcacaag tcaattttca tatgcttgtg atgtaatctc
     2341 tgatcctgct gaaaactctt ccgaaccaaa tgaatattta cttttaggta atgttttgaa
     2401 gaatcaatat ttgaataatt ttgaaaattc tataaactac tcaaatcagt tgctatcatt
     2461 gcaaaattca gaaataaatt ctattatttc attgaataat ctttttacat caaacataca
     2521 aactggtggg aatttcaatg atcttagaaa ttattattta aatcaaataa atttaaatcc
     2581 tgaagataca ataaaaaaca acaaactatt aaaactttct attgaaagta aaatagtaat
     2641 gaatgaatta gaatctgcag ttttggaata tgatgaaatt ttaaataata atttagatga
     2701 agaaataaga ttttacacat ctattgaaag aagcagagca attaggttat tattagacag
     2761 tttgttaagt ttttatcagg gagataacat aagtcaggat ctgaaacatt tatacgagaa
     2821 tgcaataaat gtaaaaagta atccaaattt tatgaatcaa tataataatt tgcataatga
     2881 agattcaaga aatattgaat ccaataaaac caataaatcc aagaatagca ttaaagctgt
     2941 aaataatttt aatgctttaa gaaataatat taatgaaaga atttctaatc ttgataattt
     3001 aaacgatagg gataaaagaa atttaattgt tgaaaagaat attttggatc tggcaataag
     3061 caatcaacta gataatattg cttcaataaa agataaactt cagtatttaa atcaaaagga
     3121 gattgtagta cctttaacat ataaactttc acaaaattat cctaatccat ttaatcctac
     3181 tactaaaatt aatttttcaa ttccaaaaga tggtaaagta gtattaaaga tctatgatgt
     3241 ttccggaaaa gaagtatata cacttgttaa tgaattcaaa caagccggta attacaatgc
     3301 aatctttaat ggatcaaatt tatcaagcgg tgtttatttt tacagattga atgctggtga
     3361 ttttgttgag tcgagacgaa tgatcttgct gaaatagttt tttcagttaa tagttagtat
     3421 ttaatagtta gtaaatttgt atttttaaaa cagaagacaa aattatttat tttaaaatct
     3481 tatacaggat attgttaaaa acggtatcct gtattttata atagaatatt taaaataatt
     3541 ttttatttaa ataaatgaag aattaaaaag ttaaaaaaat actaatcatg aattatatag
     3601 cattaataaa aaaatacggt aatggataca gcgcttatat acctgatgtt ccgggttgcg
     3661 tatctgctgc cggtacaaaa aaggaaactt taaaactatt aaaagaagaa ctgattattc
     3721 atcttcatga ttgttctgat aacacttctg aagaaaccgg atcgaaatcc gttcgactct
     3781 ctaaagatgt taaaattatt acaaatcctt cctgaacatt tccataagct tctccggctc
     3841 tttaagagaa tgaaatccga atttctcata caaagaatgc gcatctttag tagcgagaaa
     3901 ccaccttctt aaatttttta aatcgccgta attcagtatg actttcatca tccatttgga
     3961 aagtcccttt cctctatgct cctctaatat aaagacatca gcaagatatg caaaagtagc
     4021 tttatcagta attacccttg caaaaccaac ctgagttttt ttataataaa gaccaaagca
     4081 tacagaattc ccgatcgatt tttttgtagt ttttaaaagc ctattctttg cccagtaaga
     4141 atggctcaga tatttctgaa taacttttat gttgagaagt gatttatccg agcttatcag
     4201 ataatcacct ttagtgaaat ttgttttcat aaaaaaattt atgtaagata ataaatttaa
     4261 ttaaaacata ttatttaact tttaataacg agactttata attattttta cgcgtgattt
     4321 caacaataaa ggccgtttta atttcgatcc attgcgttat aatcgcaata cttacaatta
     4381 ttgtatcccc catagacagg cgaggcagga tcacacatta tctctccaaa gtatttggag
     4441 gagtaatttt atttatagcc ggagtaaaag tcactgtttc aggaacagag aatctgaata
     4501 aagatgaaac ttacattttc atttcaaatc atcttagtta ttttgacatt ccgatactga
     4561 tgaaagcaat tccgaataat gtcagattta tttataagga cagtctgaca aagatcccga
     4621 tactcgggtg gggaatgtac ctcggtggtt atattccgat cagcagggaa aatattagag
     4681 atgcgatgaa atctctgaaa gcagcagcag taaaaataat taacggaatt tctgtagtga
     4741 tatttccaga agggacgcga agttatgacg gcattccggg tgaattcaaa agaggaatgt
     4801 ttgtattggc agaggaatcc aaagtaccac tggtgcctac tacgattaca ggctcggaaa
     4861 aaattatgcc cagaggaaaa tttgaaataa gatcaggaga tgtacatgtg aattttagtt
     4921 cacctgtcga ttacagaaaa gacaaagaac tacttgatga gatcagaaac attatcgtag
     4981 agcaattaaa aattaaaaat taaaaattta tttaaatgcc tgttacaata aaagacgttg
     5041 agaaaattgc caagcttgcg aaattaaagt ttagtgaaag tgaaacaggt aagctgcaga
     5101 cagagcttaa tcaaattctc gaatacattg atcagcttaa tgagctggat ctcgaaggca
     5161 tcgaaccatt ggaaaatata aatgagactt caaatgtttt aagaaaagat gattcggaaa
     5221 aatggcttac cactgaagaa gcattaaaaa atgcgccttc aaagacaggt aaatttttta
     5281 aagtaccaaa ggtgctggat aaatgataat agccggtgtc atacccgcaa ggtacgcttc
     5341 cacaagattt ccgggaaaac cgctcgtgat gatagacgga aagtcaatga tacaaagggt
     5401 ttatgaacag tcacaaaaat gcagatccgt cagcaaagta ttagtagcga cagatgataa
     5461 aagaatattt gatcatgtga gatcattcgg aggtgaagca gtaatgactt ccgtcaaaca
     5521 ccggtccgga acggacagaa taaacgaagc cgtaaaaaat attaaatgtg atatagtcgt
     5581 gaacatacag ggtgatgaac cttttattca tccgggaaat attgataaag caatagaacc
     5641 ttttttaaaa gataaaaaat taaatgtttc cactttagct ataaagttca aagacctttc
     5701 tgaaatcaaa gatccgaatg ttgtgaaagt tatttttgat ataaataatt atgcaatata
     5761 tttttcaaga ggaatcttac cttatgataa ggatttaatt agctctgtaa atgaaagatc
     5821 aactacaaaa aaatattata agcatatagg actttacgtt tacaggaaaa gctttttgag
     5881 tgagtttgta aaaatgaaga agagttatct ggaagagact gaaaagcttg agcagttaag
     5941 gatacttgag aacggtgaaa agatcaaagt cattttaaca aataaaaatt caacagcagt
     6001 agatacacct gaggatcttt tgctgattaa aaagtaatca aataaataat caaaaataaa
     6061 ttgagagaag ttttttaatt cagtatccgc agagtctgaa gagcacgtga ctcttcggga
     6121 acacattgtg gataataata aagttggttc attttttaaa aaattaaaat ttaataagtt
     6181 ttg
//
LOCUS       DAORVQ010000194         6161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8266_length_6161_cov_5.443334, whole genome shotgun sequence.
ACCESSION   DAORVQ010000194 DAORVQ010000000
VERSION     DAORVQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_6161_cov_5.443334"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="PLX80_12360"
     CDS             complement(<1..1369)
                     /locus_tag="PLX80_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21626.1"
                     /translation="MKKLKHLPVFLITAYIMYSLGFNIMSGLYTPVTLQEVQTKSADS
                     LALGRDRSNLEGDSVQFVLRVVAPPRVSPANNDFRTMMRGTTSWTCYGQDTANGVFGG
                     IVIRQNGRESQTAIDLIDSGSIIRVRGQIQEFGATSGAPFANMLTQCALDIETGYTID
                     ILESGSNSKRPAPIPVTIPTFANGDYPNGGSINYIDGEKYEGMYIEIRNVTTAGGIGN
                     RQPWSIIDENGNRMYIRDFSNFFSTQPSGDTLRSWTHPSPGTFVEYIRGVVINANNEG
                     AFGSQLPYVIVPIYPNDLSLGNAPPQLSSPTRSPGVPTPPDSVTVSVTAIDPANSATI
                     TDMKVFFRLNNGAFQSKNMPLITGNIYSTKMPPANLGTLIEYYFRAEDNSGGVKLLPS
                     DTNRSTLFYEVRSNDSMGIRDVQFCPNNGGRSAFEGFDVRGVEGIVTADTSDIPGINF
                     SSAGGN"
     gene            complement(1450..3567)
                     /locus_tag="PLX80_12365"
     CDS             complement(1450..3567)
                     /locus_tag="PLX80_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21627.1"
                     /translation="MSQRILYLFFFSLKKIKSHRSKLLFLFVLTVLFSSYYYPGDIQD
                     DVNNKFTNVGNISMTITNYGTIGNGFVNFPNQPSCQYPKGSGIENIFVGGIWVGGAKG
                     GQIRVSTGAIDVSSANRGEGFEFSNSPGQGILERSSLQNSPNYRPDAVSHQDFVCEFL
                     DTNTSIGGTPIVNHVPLGVKVLLESYCYDFNFANNWVILNYKLVNIGYNGDTSPIDSI
                     YVGLWADAVVRNTNISPPGGTSFYNKGADGYDSSLRMWYEYDYNGDPGFTDTYVAFKL
                     LGLTPKPAIENINSKTHFSIWQFRNATGILYLSPIEDQSNQAGGGKFEKMNGYLSVSP
                     LDSLSNPFVPSRNDELRHSPNNRSTIISYGPFHKDNGDKLSLRYLQDTINIVFAVVCS
                     KKSGTEPTTWDSASQRQNLNVSAGWAQSAYDNGYRLPSPPDIPQLKTVIADKNVTLYW
                     AANSENSIDPISNKKDFEGYKIYRTNPGSDLSQTIDLTDQLKLVGEFDSAYNNISNNT
                     GFNFIKLDAPKYFEGDATPYYYRFDFPNQLNGFQYVYSVTAFDKGDITQNLGSLESSI
                     LGNSQRIIVGTPANNDVSAEVGVYPNPYYGSAVWDGTGNKKELLRKMYFYNLPSKCDI
                     SIWTISGDLVDQFQHDAATYNGSDIAWFTTYSDGTQKFAGGEHAWDMITNSEQALASG
                     MYFFTVKDQNTGEVKKGKFVIVK"
     gene            complement(3663..5147)
                     /locus_tag="PLX80_12370"
     CDS             complement(3663..5147)
                     /locus_tag="PLX80_12370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY21628.1"
                     /translation="MKFILLIIIILTAVLRIYDIEIKNMWFDEVYSWKLSQKSVTVIV
                     SDTSGDIHPPFFYIVLKYWTELFNDSILSMRLLSVLTSLLSIIFIYKISKIVFNKNYL
                     VIFILLLYAVSPVNIFYSQEVRMLSLNLFLCLGSVYYFLISFKDSSFKNLLLYILFSV
                     LAIYTHYFAFLIIFTEFIILILKVFTKTEKFSETKKLFISVFLINLFYLPWYPVFFQQ
                     VSKGQPWRTVLTFKDSGINFLDYFKHIFLSNYYTFEPGYIHFLATFITYFILAFLLYS
                     LFRFFNSKVKLMKDNNYTILFFFIPLIIAGIISIKQNILLSRYLSIILPFLLITIMFF
                     SDKYLKRKLTVTLFIFLFSISSLGVYINYRNEFKNNDYRKIINYIEENFTDGDQILTE
                     PHFMGWSIEYYKEHSQTKLPVPVIFGWNIDMQLDSINNRNDLKNLWIIQDYSSLENEK
                     YDSTISRMENMGYIKSKDKTFYPVPSKVNVSKFIRPEIKNERKY"
     gene            complement(5166..5585)
                     /locus_tag="PLX80_12375"
     CDS             complement(5166..5585)
                     /locus_tag="PLX80_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQY21629.1"
                     /translation="MKILTKELIIESRGNCDIIDITDEVQRILNASNMLEGICNIFSI
                     GSTASVTTIEFEPGLLQDIPKILEKLIPTYSRYHHNDTWGDGNGHAHIRSAIIGTSLN
                     VPFNSGMLMTGTWQQIVLIDFDNKKRTRRVAVQLTGI"
     gene            complement(5554..>6161)
                     /locus_tag="PLX80_12380"
     CDS             complement(5554..>6161)
                     /locus_tag="PLX80_12380"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQY21630.1"
                     /translation="NLIGVSSLNKSNNLFLRKSIKKHFGKESIFINNKIHLPIKIEYT
                     SSIGNDRICNAVAADNLFPGKNILVIDFGTATTYTLVSEKILKGGIISPGILTAFNSL
                     KDKTSLKFSNISFPEKLIGQTTEDNIKSGIMNQTLIFCEYVINEIRKSHRGLKVIITG
                     GFSEFMSNRSKLFDLTDRELVFKGVNLIIKYNENINKRINN"
BASE COUNT         1976 a         1067 c          996 g         2122 t
ORIGIN      
        1 gatttccgcc tgcgcttgaa aaatttattc ccggaatgtc cgaagtatct gctgtaacaa
       61 ttccttccac tcctcttacg tcaaatcctt caaatgcaga ccttccgccg ttattcggac
      121 agaactgtac gtcacggatt cccatagaat cgtttgatct tacttcataa aataatgttg
      181 atctgttagt gtctgacggc agtaatttta cacctcctga attatcttct gctctgaagt
      241 aatattctat taatgttccg agattagccg gaggcatttt tgtggaataa atatttcccg
      301 ttataagagg catattcttg ctttgaaaag cgccgttgtt taacctgaaa aaaactttca
      361 tgtctgtgat agttgcggaa ttcgcaggat caattgcagt tacactcact gtcacactgt
      421 ccggaggagt aggcacgccg gggcttcttg tcggacttga aagctgcggc ggtgcatttc
      481 caagactaag atcatttgga taaattggga ctattacata aggaagctga cttccgaaag
      541 ctccttcatt gtttgcattg attacaactc ctctgatata ttccacgaaa gttccgggtg
      601 aaggatgtgt ccatgatctc aatgtatcac ctgaaggttg agtggaaaag aagtttgaaa
      661 aatctctgat gtacattctg tttccgtttt catcaattat actccagggt tgtctgtttc
      721 caataccgcc ggcagtagtt acgtttctta tctcaatata catgccttca tatttttctc
      781 catctatata atttatcgaa cctccgtttg gataatcacc gtttgcaaag gttggaatag
      841 taaccgggat tggtgccggt ctttttgagt tacttccaga ttcaagtata tcgatagtat
      901 agcctgtttc tatatcaaga gcacactgtg ttagcatatt agcaaatgga gcaccacttg
      961 tcgcaccaaa ttcctgaatt tgtccgcgaa ctctgattat tgaacctgaa tcaataaggt
     1021 caatagccgt ctgtgattcc cttccgtttt gtcttattac aattccaccg aatacgccat
     1081 ttgctgtatc ctgaccgtaa caagtccagc tggttgtgcc tctcatcatt gtcctgaaat
     1141 cgttatttgc aggggatact cttggaggtg caactactct caaaacaaac tgcacagaat
     1201 ctccttcgag atttgacctg tctctgccga gtgcaagtga gtctgcagat ttcgtctgaa
     1261 cctcctgtaa tgtaacaggt gtataaagac cggacattat gttaaatcct aaagaataca
     1321 taatgtaagc agttattaaa aaaaccggta agtgttttaa ttttttcatg atgattttat
     1381 tttatttaaa tatatttttt aatttagttt gaatgtttta tcaattttta aactcatata
     1441 aaattaagat tattttacta tcacaaattt acctttcttc acttcacctg tattctgatc
     1501 tttgactgta aagaaataca ttcctgatgc taatgcctgt tcgctgttag tgatcatatc
     1561 ccatgcgtgc tcaccaccgg caaatttctg agtaccgtct gaataagtag taaaccatgc
     1621 aatgtcactg ccattatagg tggcagcatc atgctgaaac tgatcaacga gatcacctga
     1681 tattgtccaa atagaaatat cacacttcga tggcagattg taaaaataca ttttcctcag
     1741 tagctctttt ttatttccgg tgccgtccca tacagcacta ccgtaatatg gattaggata
     1801 gacacctact tcagcagaaa catcattatt tgcaggagta ccgactatta tccgttgtga
     1861 atttcctaag atactacttt caagtgaacc aaggttctgt gtaatatccc ctttatcaaa
     1921 agctgttaca gagtagacat actgaaaacc attaagctga tttggaaaat caaatctgta
     1981 ataataaggc gttgcatctc cttcgaaata tttgggggca tcaagtttta taaaattaaa
     2041 tccggtgtta ttactaatat tattataagc gctatcaaac tcaccgacaa gttttagctg
     2101 atctgtaaga tcaattgtct gactaagatc cgaacccgga ttggttctgt atattttgta
     2161 cccttcaaag tctttcttat ttgatatcgg atcaattgaa ttttcggaat ttgctgccca
     2221 gtataatgtc acattcttat ctgcgatgac tgtcttaagc tgaggaatgt ccggcggaga
     2281 tggaagtctg tatccattgt cataagcact ttgtgcccag ccggctgaga catttaaatt
     2341 ttgtctctgt gatgcagaat cccatgtcgt aggttctgta cctgattttt ttgaacatac
     2401 tactgcaaat acaatattaa ttgtatcctg aagatatctt agtgaaagtt tatctccgtt
     2461 atctttatga aatggaccat atgaaatgat tgtagatctg ttattcggtg aatgtctgag
     2521 ctcatcattt ctcgaaggta caaatggatt acttaatgaa tcaagagggg atacacttaa
     2581 atatccgttc atcttttcaa attttcctcc gcctgcctga ttactttgat cctcaatagg
     2641 agataaatac aaaattccgg tagcatttct aaattgccaa atagaaaaat gagttttgct
     2701 gttaatattt tcaatagccg gctttggtgt caatccaaga agcttaaatg caacataagt
     2761 atctgtaaat cccggatctc cattgtaatc atattcatac cacattctta aagaactgtc
     2821 atatccgtct gctcctttat tgtaaaatga agtacccccc ggtggactaa tgttggtatt
     2881 cctgacgaca gcgtcagccc ataatcctac ataaatactg tcaataggtg aagtgtcacc
     2941 attgtagcca atattaacta atttataatt taatattacc cagttgtttg caaaattaaa
     3001 atcatagcag taagattcga gcagtacttt cacaccgagc ggaacatgat ttacaatcgg
     3061 tgtaccgcca attgaagtat ttgtatcaag aaattcacag acaaaatcct ggtgtgagac
     3121 tgcgtccggt ctgtaattag gtgaattttg aagtgaagac ctttctaaaa ttccctgacc
     3181 cggtgaattt gaaaactcaa atccttctcc gcggtttgcg cttgaaacat caattgctcc
     3241 tgttgaaacc cttatctgtc caccttttgc gccgcctacc caaattccgc caacgaatat
     3301 attttctatt ccagaacctt tagggtattg acaagaaggc tgatttggaa aattaacgaa
     3361 tccatttcca attgtaccat aatttgtaat agtcatggaa atatttccga catttgtaaa
     3421 tttattattt acgtcatcct gaatgtcacc cggataatag tatgatgaaa atagtactgt
     3481 taaaacaaat agaaaaagaa gttttgatct gtgggatttt atttttttaa gtgaaaaaaa
     3541 aaataaatag agtattcttt gggacattaa ttttattaat atttagagtg aaatttgatt
     3601 taaattaagt taattatcag taaaagtaaa gataaagaaa agtggtattt aataactatt
     3661 tcttaatatt tcctctcatt ttttatttca ggtcttataa atttggatac atttactttt
     3721 gagggtacag ggtaaaaagt tttatctttt gatttgatat aacccatgtt ttccattctt
     3781 gaaattgttg aatcatattt ctcattttct aatgaactgt agtcctgtat aatccacaag
     3841 ttttttaaat cgttcctgtt gtttattgaa tcaagctgca tatcaatatt ccatccgaat
     3901 attacaggta caggtagttt tgtttgagaa tgttctttat agtattcaat actccatccc
     3961 ataaaatgag gctctgtaag tatttgatcg ccgtcagtaa aattttcttc tatatagtta
     4021 attatttttc tgtaatcatt attcttgaat tcattccggt aatttatata aacccctaaa
     4081 ctcgaaatac taaaaagaaa aataaacaga gttacagtta attttctttt caaatatttg
     4141 tctgaaaaaa acataattgt gattaacaaa aatggtagaa ttatactcag atacctggaa
     4201 agtaaaatat tttgcttaat ggaaattatt ccagctatga ttaggggaat gaaaaaaaac
     4261 aatatagtat aattattgtc tttcattaat ttaacctttg aattaaaaaa cctgaacaaa
     4321 ctgtataaaa gaaatgccag tataaaatat gtaatgaaag ttgccaggaa gtgaatgtaa
     4381 ccgggctcaa atgtgtaata attacttaaa aaaatatgtt taaaataatc cagaaaattt
     4441 attccactat ccttgaatgt taagactgtt ctccatggct gccctttaga tacttgttga
     4501 aaaaatacag ggtaccaggg taaataaaac aaatttatta aaaagacaga tatgaatagt
     4561 ttttttgttt cggaaaattt ttcagtttta gtgaatactt ttaatattaa gattataaat
     4621 tcagtaaaaa tgattaagaa agcaaaataa tgtgtataga ttgctaaaac agaaaagaga
     4681 atatatagaa gtaaattttt aaaagagctg tccttaaatg aaattaaaaa ataataaacc
     4741 gatccaaggc ataaaaagag atttagactc agcatcctta cttcctgtga ataaaaaata
     4801 tttacaggtg acactgcgta tagtaaaagt ataaaaatta ccaaataatt tttattaaaa
     4861 actatttttg aaattttata aatgaaaata attgaaagca gggaagtgag tacactgagc
     4921 aatctcattg ataaaatgct gtcattaaat aattctgtcc agtactttaa aacgatataa
     4981 aaaaatggag ggtgtatatc tcctgatgta tcagaaacaa ttactgtaac tgatttttga
     5041 gatagcttcc aggaatatac ttcatcaaac cacatatttt tgatttcaat atcatatatt
     5101 ctcaaaaccg ctgtaagaat aataattata agtaatataa atttcatttt cacgaaatta
     5161 gtgtcctata ttccggtaag ctgaactgca actctgcgtg ttcttttttt attatcaaaa
     5221 tcgatcagca ctatctgctg ccaggttcct gtcataagca ttccactgtt aaacggaaca
     5281 tttagagatg taccgattat cgcagacctg atatgtgcat ggccgttacc gtctccccaa
     5341 gtatcattgt gatgatacct ggaataagtt ggaattaatt tttctaaaat ttttggaatg
     5401 tcctgtaata aacccggttc aaattcgata gtggtgacag aagcagttga gccgattgaa
     5461 aaaatattgc aaatcccttc aagcatattt gaagcattta aaattctttg tacttcatca
     5521 gtaatatcaa taatatcaca attaccacgg ctttcaatta ttaattcttt tgttaatatt
     5581 ttcattatac tttattatta aatttactcc tttaaaaacc agctcccggt cagttaaatc
     5641 aaaaagtttg gatctgttac tcataaattc actgaagccg ccggttataa ttacttttaa
     5701 cccacgatga gattttcgta tttcatttat tacatattca caaaaaataa gagtctgatt
     5761 cattatgcct gatttgatat tatcttctgt tgtctgacca attaattttt caggaaaact
     5821 gatatttgag aattttaaag atgttttatc ttttaaagaa ttaaatgccg tcaatattcc
     5881 gggtgagatt attccgcctt ttaaaatctt ttcagagacc aaagtatagg ttgtagcagt
     5941 tccaaagtct attacaagaa tgttttttcc agggaataaa ttatcagcag ctacagcatt
     6001 acatatccgg tcatttccaa tactactcgt atattcaatt tttattggaa gatggatttt
     6061 attatttatg aaaattgatt cttttccaaa atgttttttt attgattttc ttaaaaataa
     6121 attattgctt ttgtttaaag aagatacacc tattaaatta a
//
LOCUS       DAORVQ010000195         6140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8312_length_6140_cov_5.698767, whole genome shotgun sequence.
ACCESSION   DAORVQ010000195 DAORVQ010000000
VERSION     DAORVQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8312_length_6140_cov_5.698767"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1455
                     /locus_tag="PLX80_12385"
     CDS             52..1455
                     /locus_tag="PLX80_12385"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HQY21631.1"
                     /translation="MKTFNKFFAVSLLAFMSVFFMKSDSNADVYTFTESLSGLEEVPP
                     NASTGTGLLTGTYDDGTNALSFNVSFSGLSAGTTAAHFHGPAAVGVNAGVQIGFTGFP
                     TAVTSGVYNNNFILTASQETDFLAGLWYVNVHTTALPGGELRSQLNPAVILSANVYAI
                     SETLSGLEEVPPNASPGSGLLTGTYNDGTNVLSFSVSFSGLTANTTAAHFHGPAAVGV
                     NAGVQIGFTGFPTGVTGGVYINNFVLTASQESDLLANLWYVNIHSSVFPGGEIRSQLF
                     PDIVLPVELSSFTSVISGNDVILNWTTAAEINNSGFDIERKSNDLVWDKIGFVSGQGN
                     SNEIHSYIFTDNDLNTGVYNYRLKQIDLNGNFEYFNLSNEVNIGVPADFDLSQNYPNP
                     FNPETKIDFTIPENGNVSLQLYDYTGRLVKTLFGGFKSAGYYSYKLNASDISSGIYFY
                     KLESGNQSITKKLMLVK"
     gene            1638..2258
                     /gene="purN"
                     /locus_tag="PLX80_12390"
     CDS             1638..2258
                     /gene="purN"
                     /locus_tag="PLX80_12390"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQY21632.1"
                     /translation="MRICVFASGTGTNFKAILLSRKRNKIKSDINLLISNNSDCGAVK
                     IAKESGIDFKIINRNFYPGLSDKNFDDLFLKELRKSKIDFIVLAGYMKLLNKEVLRKY
                     KNRIINIHPALLPSFGGKGMYGANVHRAVIASGAKVSGITIHFADEKYDEGKIIFQKC
                     CEVDFEDDENSLSQKIRKLEHKFYPEIIRKFEEKKVLILKNKVKVI"
     gene            2260..3798
                     /gene="purH"
                     /locus_tag="PLX80_12395"
     CDS             2260..3798
                     /gene="purH"
                     /locus_tag="PLX80_12395"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQY21633.1"
                     /translation="MIKRALISVYDKEGIIEFAQDLRNAEVEIISTGGTYKLLSENGF
                     EITKVEDITDFPEILNGRVKTLHPKIFGGILSDRGNDEHNSDSEKHGLGMIDLVVVNL
                     YPFEDTVMKENVTEEEAVEQIDIGGITLIRAAAKNFKHVNVIVSKYQYEEYLRTFKQT
                     GNNIPLEYSRMLAYKAFKTTSHYDNEIKNYYYKNIFDKESDFIDVPDLNSFESTGLRY
                     GENPHQKAVLLKNDFDEIFEVLHGKELSYNNILDIDAAYSLVSDFKDSEPFCGIIKHG
                     NPCGSAVSGNIQEAYRKALETDTVSPFGGIIIFNQKLDFKTSIDIDKLFIEIILAPDF
                     ENEALDLLKKKKNRRLIRFKFKNEITEFRKISGGVLLQGKDNIFSENGRLNFVTIKKP
                     DRKAEEDLIFAEKIAKHVKSNAVVFVKDKRTLAIGGGQPSRIDSTRIAVMKAKEFDLD
                     LQGSVCASDAFFPFADGLLEIAKAGASAVIQPGGSVRDEDIIKAADENGISMAFTGIR
                     HFKH"
     gene            3843..4859
                     /locus_tag="PLX80_12400"
     CDS             3843..4859
                     /locus_tag="PLX80_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQY21634.1"
                     /translation="MPLLGMFSNDIAIDLGTANTLIYMKGKGIVLNEPSIVTYDVTSR
                     KIIAIGEESKKMMGRVHKELNTIRPMKDGVIADFEIAEGMIKEFIKKISSSWMPSRRI
                     VICVPSGITEVEKRAVRDTAEHAGAKEVYLISEPMAAAIGIGLDVMAPYGNMIVDIGG
                     GTTEIAVIALSGITNGTSIRIAGDELTDAIIRFFRNNHNILIGERTAEEIKCQVGSVM
                     PLKEEVIIEVKGRDLVAGIPKITEVSSIEIRESLNAPVNQMIEAVRVALEKTAPELAS
                     DLLDRGIFLTGGGALIKGLDERIKMETGVPVYVAEDPLTAVARGTGKVLEDLDYYKKV
                     LLRR"
     gene            4952..5779
                     /gene="mreC"
                     /locus_tag="PLX80_12405"
     CDS             4952..5779
                     /gene="mreC"
                     /locus_tag="PLX80_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQY21635.1"
                     /translation="MKTIGLFLLSIKEYLIFSLVIIISLILIFSNDNSQVRFFRAIAV
                     GFIGTIQSGVSAVPNVFEIQKENQFLREKNIELSNKLSELKEAKLENIRLTKLLGLKN
                     SNISGIIIASIVNKSLIQARNTITLNVGESDSIKINMPVITDDGLVGRIVSTSKNYSI
                     AQILLNRNMNISVKVQRSRVDGILNYDGSENVTISNIAKSADVKAGDVILTSEYSNYF
                     PAGIPVGIVTEEGNLDNLFKKVIVKPLVNFTSLEEVFVLKRLPENERVMLEEYYLQK"
     gene            5797..>6140
                     /gene="mreD"
                     /locus_tag="PLX80_12410"
     CDS             5797..>6140
                     /gene="mreD"
                     /locus_tag="PLX80_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQY21636.1"
                     /translation="MYFSEYLKFAIIFAVLIIIQVSFVWLISISSYNITPDLVIILVI
                     YIGFTRGHIAGMLSGFFTGLTLDLLSGSFIGLSALSYSIAGFIAGYFYFQESSKSTVK
                     RSMIGIMSLCTVV"
BASE COUNT         2131 a          892 c         1213 g         1904 t
ORIGIN      
        1 gatttgaaat ttaatttagt aaattattta ttaattaaac tttttaacaa aatgaaaact
       61 tttaacaaat tttttgcagt atctttactg gcatttatgt ctgtattttt tatgaagtca
      121 gactcaaatg cagatgtata tacttttact gaatcattgt caggacttga agaagtacct
      181 ccaaatgcat caacaggtac aggtttacta acaggtacat atgacgatgg tacaaatgca
      241 ctttcattta atgtatcttt cagtggatta agtgccggaa caacagcagc acattttcac
      301 ggacctgcgg cagttggagt aaatgcagga gttcaaattg gatttacagg ttttcctaca
      361 gcagtaacaa gcggtgttta taataataat tttattctta cagcatccca ggaaacagat
      421 tttcttgccg gcctttggta tgtgaatgtt catacaacag cactgccggg tggagagtta
      481 cgatcccagc ttaatcctgc agtaatactt tctgctaatg tttatgcaat ttctgaaact
      541 ttatccggtc tggaagaagt acctccaaat gcgtcacccg gttcaggttt attaacaggg
      601 acttataacg atggtaccaa tgtactttct tttagtgtat cttttagtgg tctaactgca
      661 aatacaacag cagcacattt tcacggacct gcagcagttg gagtaaatgc aggagttcaa
      721 attggattta caggatttcc taccggagtt accggtggtg tttatattaa taattttgtt
      781 cttactgcct cacaggaatc agatctgctt gcaaatctct ggtatgtaaa tattcattca
      841 tcagtatttc ccggcggcga aataaggtct cagctatttc cggatatcgt attgccggtc
      901 gagctgtctt cattcacttc agtaatttcc ggaaatgatg taatacttaa ctggaccact
      961 gctgctgaaa taaataactc gggatttgat attgaaagaa aatcaaatga ccttgtatgg
     1021 gataagattg gttttgtaag cggtcaggga aattcgaatg aaatccattc ttatattttc
     1081 acagacaatg atctgaatac aggagtatat aattacagac taaaacagat cgatctgaac
     1141 ggaaattttg aatattttaa tttatcaaat gaagtgaata ttggggtacc tgcagatttt
     1201 gatctttctc aaaattatcc aaatcctttt aatcctgaaa ctaaaattga tttcacaatt
     1261 cctgaaaatg gaaatgtaag tcttcagtta tatgattaca caggaagact ggtgaaaact
     1321 ttatttggag gatttaaatc agcgggatat tattcataca aattaaatgc atcagatatt
     1381 tcaagcggaa tttattttta taagctggaa agcggaaatc agagcattac caaaaagctt
     1441 atgcttgtaa aataaaattt ctttatagtt aaatttcaaa ttaaaaagcc ggagatatta
     1501 tccccggctt ttattttgtt aaaaactcat tatatattaa aaattgaaaa ttgaacattg
     1561 aactttttac attaaacatt gaacattaaa ttgtatatac tttttaaatt gattaatttg
     1621 catacaaaac aacagaattg agaatttgtg tgtttgcttc aggtacaggg acaaatttta
     1681 aagctatact tctttccagg aaacgaaata aaataaaatc cgatattaat ttgctgatct
     1741 caaataattc tgattgcggt gcagttaaaa ttgctaaaga atcaggaatt gattttaaga
     1801 tcattaacag aaatttttat cccggattat ctgataaaaa ttttgatgat ctatttttaa
     1861 aagagttaag aaaaagtaaa atagatttta ttgttcttgc agggtatatg aaattattaa
     1921 acaaagaagt tttaagaaaa tataaaaaca gaataataaa tattcaccct gctttgcttc
     1981 cttcattcgg aggaaaggga atgtatggtg caaatgtaca tagggctgtc atagcaagcg
     2041 gagcaaaggt ttccgggatc accatacatt ttgcagatga gaaatatgat gaagggaaaa
     2101 ttatttttca gaaatgttgt gaggtggatt ttgaagatga tgaaaattct ctttctcaaa
     2161 agatcagaaa acttgagcat aaattttatc cggagataat ccgaaagttt gaagaaaaga
     2221 aagttttaat tttaaaaaat aaagtgaaag taatttaagt tgataaaaag agcattgata
     2281 agtgtatatg acaaggaagg aataattgaa tttgctcagg acttgaggaa tgcagaagtt
     2341 gagataattt ctaccggagg tacatataaa ctgctttcag aaaatggatt tgaaattaca
     2401 aaagtggagg acatcacaga ctttccggaa atattgaacg gcagagtgaa gacccttcat
     2461 ccgaaaatat ttggtggtat tctcagtgac agaggaaatg atgaacataa ttcagattca
     2521 gaaaagcatg gtcttggaat gatagactta gtagtcgtga atctatatcc gttcgaggat
     2581 acagtcatga aggaaaatgt aactgaagaa gaagcagttg agcagatcga catcggcggt
     2641 attactctta tccgtgctgc tgcaaaaaat ttcaaacatg taaatgtaat tgtaagcaag
     2701 tatcagtatg aagagtacct gagaacattt aagcaaaccg gtaataatat tcctttagag
     2761 tattcaagaa tgcttgcgta caaagctttt aaaacgacat cgcattatga taatgaaata
     2821 aaaaattatt attataaaaa tattttcgat aaagaaagtg attttataga tgtacctgat
     2881 ttaaattcat ttgaaagtac cggattgaga tacggagaaa atccacatca gaaagcagtg
     2941 ctgctcaaaa atgattttga tgagatattt gaggtgctgc atggcaagga gctttcctac
     3001 aataatatac tggatataga cgctgcttat tctttagtca gtgattttaa agattccgaa
     3061 cctttttgcg gcatcataaa gcatggaaat ccatgcggat ctgcggtttc agggaatatt
     3121 caggaggctt acagaaaagc tttagaaaca gatacggttt caccctttgg cggaattatt
     3181 atttttaatc aaaaattaga ctttaaaact tcaatagata ttgataaatt gttcattgaa
     3241 attattttag ctccggattt tgaaaatgaa gcacttgatc ttttaaaaaa gaaaaagaac
     3301 aggcgtctta tcaggttcaa atttaaaaat gaaattacag agtttagaaa aatttccggt
     3361 ggagttttac ttcagggaaa agataatata ttttctgaaa acggcagatt gaattttgtt
     3421 acaatcaaaa agcctgacag aaaagcagaa gaagatctga tctttgcgga gaaaattgca
     3481 aagcatgtaa aatcaaatgc agttgttttt gtcaaagata aacgaacact tgcaattgga
     3541 ggtggtcagc cttcgaggat tgattcgacc aggattgcag tgatgaaagc aaaagaattt
     3601 gatcttgatc ttcagggaag tgtctgcgct tcggatgcat tttttccatt tgctgatgga
     3661 cttttagaaa ttgcaaaagc cggtgcaagt gcagtaatac agccgggcgg ttctgtaaga
     3721 gatgaagata ttattaaagc tgctgacgaa aacggaatct caatggcttt taccgggata
     3781 agacatttca aacattaaaa atttttttta taaaatttag atatatactt aaggattatt
     3841 aaatgccatt attaggaatg ttttccaatg atatagcgat agatctgggc actgcaaata
     3901 cactgatcta tatgaaaggt aagggaatag ttttaaatga accgtcaatt gttacttacg
     3961 atgtaacttc gagaaaaata attgcaatcg gggaagagtc aaagaaaatg atgggaagag
     4021 ttcataaaga acttaatacc attcgcccta tgaaagacgg cgtgatagct gattttgaaa
     4081 ttgccgaagg tatgataaaa gaatttatta aaaaaataag ttcgagctgg atgccttcca
     4141 gacggatcgt tatctgtgta ccaagcggaa ttactgaagt agaaaaacgt gcagtgagag
     4201 atactgccga acatgcaggg gctaaagaag tttatcttat ctctgaacca atggctgctg
     4261 ctataggtat cggacttgat gtgatggcac cttacggtaa catgatagtt gacattggtg
     4321 gaggaacaac agagatcgct gtgatcgcat tgtcgggaat cacgaacgga acttcgatca
     4381 gaattgcagg tgatgaactt acagatgcta ttatcagatt tttcagaaat aatcataaca
     4441 tacttatcgg tgaaagaaca gccgaggaaa tcaagtgtca ggtcggttct gtcatgcctt
     4501 taaaagaaga agttataatt gaagtaaaag gcagagacct tgttgcgggt attcctaaga
     4561 tcacagaagt aagttctatc gaaatcagag aatcactgaa tgcaccggta aaccaaatga
     4621 ttgaagcagt aagagttgct cttgagaaga cagcacctga attagcttct gaccttcttg
     4681 acaggggaat ttttctcaca ggtggcggtg ctttgataaa gggacttgat gaaagaatta
     4741 aaatggaaac aggtgtgcct gtttacgtgg ctgaagatcc actgaccgca gttgcaagag
     4801 gtacaggaaa agtcttagag gatctggatt attacaaaaa agttttactc agaagataaa
     4861 ataaagtaac atctctgctt cgatcttatg taattttgta aatcacttct tattttaata
     4921 ttgtaatata gaaattattg aaaaggtata aatgaaaact atcggtttat ttttattaag
     4981 cataaaagaa tatctgattt tttcgttggt cataataatt tcactcatac tgatattttc
     5041 aaatgacaat tctcaggtaa gatttttcag agcgatagca gttggattta tagggacgat
     5101 acagagcggg gtgtcagcag tacctaatgt atttgagata caaaaggaaa atcagtttct
     5161 cagagaaaaa aatattgaac tgtcgaataa gctttccgaa ttaaaagaag ctaagctgga
     5221 aaatatccgg cttacaaagt tacttggtct gaaaaactct aacatttccg gaataataat
     5281 cgcaagcata gttaataaat cacttataca ggcaagaaat actatcactt taaatgtcgg
     5341 tgaatctgac agcattaaaa taaatatgcc tgtaataact gatgacgggc tcgtcggaag
     5401 gatagtatca acaagtaaaa attattctat agctcagatc ctactcaaca ggaatatgaa
     5461 tatttctgta aaggtgcagc gaagcagggt agatggtatt ctgaattacg atggatctga
     5521 aaatgttacg atcagcaata ttgccaaaag tgcagacgta aaagctggag atgttatact
     5581 tacttctgaa tatagtaatt attttcccgc aggaatacct gtcggcatag taaccgaaga
     5641 agggaatctt gataatttat ttaaaaaagt aatcgtaaaa cctcttgtga attttacttc
     5701 gcttgaggaa gtatttgtat taaagcgttt accggaaaat gaaagagtaa tgctagaaga
     5761 gtattactta caaaaataag tttaaattgt acagcattgt atttttctga gtatctaaag
     5821 tttgcaatta tatttgccgt cctgatcata atacaagtct catttgtttg gctgatctca
     5881 atttcaagct ataacattac tcctgatctt gtgatcatac ttgtaatata tattggattt
     5941 acaagagggc atatagccgg aatgctgtca ggatttttta ccggacttac gcttgatttg
     6001 ctgagtggat cttttatagg actttcagca ttgtcgtaca gcatcgccgg atttattgca
     6061 ggatattttt attttcagga aagttcgaaa agtacagtta aaagatcaat gatcggaatt
     6121 atgtcactct gtactgttgt
//
LOCUS       DAORVQ010000196         6114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8371_length_6114_cov_7.292953, whole genome shotgun sequence.
ACCESSION   DAORVQ010000196 DAORVQ010000000
VERSION     DAORVQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8371_length_6114_cov_7.292953"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..2132
                     /locus_tag="PLX80_12415"
     CDS             429..2132
                     /locus_tag="PLX80_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQY21637.1"
                     /translation="MLVPKLFTTLKTYNKKQFYKDVIAGIIVGIIALPLAIAFGIASG
                     VSPEKGLITAVIGGFLISVLGGSRVQIGGPTGAFIVIVYGIVHNYGLDGLMIATIMAG
                     VILIIMGFMKLGNLIKFIPIPLIAGFTSGIALIIFTTELKDLFGLKIENLPAEFLEKI
                     PVYIQNFNTINTDTAIISLITLLIIIFLPYVTRKIPAIFLALVVGTLVTHFFGFNVET
                     ISSRFGEISNSIPSPSFPVFSYTKVYALLGPAFTIAILAAIESLLSAVITDGMTGGRH
                     RSNMELIANGAANIASPIFGGIPVTGAIARTVTNIKSGGSTPVAGIIHSLILFLVIIF
                     FGKYAGMVPLSVLAAILVAVAYNMFDWREFRSQLKMPKSDVIVFLSTFFLTILFDLTI
                     AIEIGMILSAFLFMNRMAKVANIGIITRELNDDSDTEDAEINSIQNKNIPDGVVIYEV
                     NGPFFFGAAGKFNDVLRTIHSKSKILILRLRNVPAIDATGINTIENIYEDCRNKKSVL
                     ILSGIHAQPLFAAEKAGLIEKIGIENVKGNIEDALNRAKEILGIPIDTMNQDDESSEG
                     K"
     gene            complement(2196..5861)
                     /gene="metH"
                     /locus_tag="PLX80_12420"
     CDS             complement(2196..5861)
                     /gene="metH"
                     /locus_tag="PLX80_12420"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQY21638.1"
                     /translation="MNDIKEILKNRILVLDGAMGTMIQRHILEEEDFRGERFKDHSHP
                     LKGNNDLLSITRPDIIKEIHTEYFKAGADIAETNTFSSTTIAQADYKLEHIVYELNFE
                     SAKLAKEVADEFTKNEPEKPRFVAGSMGPTNRTASLSPDVNDPGFRAVTFDQLMEAYY
                     EQAKGLMDGGVDILLVETIFDTLNAKAALFAIEKLFEDTGKKIPVMVSGTITDASGRT
                     LSGQTVEAFLYSVNHLELLSIGFNCALGAKQLRPHISTISEKSDLLISAHPNAGLPNE
                     FGEYDETPESTAKQIEEFLNEGLLNIVGGCCGTTPEHINAIYEVTKNFKPRVPPVITD
                     IPRFSGLEPLEVYKGSNFVNVGERTNVTGSAQFKKLIIDEKYEDALAIARHQVEGGAQ
                     VIDVNMDEGMLDSEEVMTKFLHLIGSEPDIAKLPIMIDSSKWSVIEAGLKCVQGKAIV
                     NSISMKEGEEKFIESARKVRMYGAAVIVMAFDETGQADSYERKIEICQRAYDILTKKV
                     GFNPSDIIFDPNILTVATGIEEHNNYAVDYIQATKWIKENLPGAQVSGGVSNISFSFR
                     GNNVVREAMHSAFLYHAIKAGMDMGIVNPGMIEVYEEIPKDLLERVEDVLLNRRPDST
                     ERLVEFAENIKHKGKVQVKDETWRKMDVKKRLTHSLVKGIVDYIVEDTEEARQMFDKP
                     IEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKKAVAYLLPYIEAEKTVDSKPK
                     GKILMATVKGDVHDIGKNIVGVVLGCNNYDVVDLGVMVPCEKILEAARNEKADIIGLS
                     GLITPSLDEMVHVAKEMEREGFDIPLLIGGATTSKVHTAVKIEQNYKRGQTIHVLDAS
                     RSVPVVGNLLNENKGAFESGIKDEYTKLREHHLKNKVQKNYLKYEDAKKNKFEIDFKK
                     EDIAVPEFKGIKILNDYPLQEIAEYIDWTPFFRTWELAGKYPEILEDKVVGEQAANLF
                     LDAKNLLNIIIKEKRLTANAVFGIFPANSVGDDIEVYTDESKTEVLKTIHTLRQQNEK
                     SKGQPNLALSDFIAPKESGLTDYIGGFAVTAGMGIEEWIKKYNEDHDDYNKIMMQALG
                     DRLAEAFAEMLHKKVRTEYWGYDKNEKLENEDLITEKYRGIRPAPGYPACPDHTEKWT
                     LFELLDAEKNTGIKLTESLAMYPASSVSGMYYAHPESKYFGLGKISKDQAEDYAKRKL
                     MTIEEVERWLSPVLNYD"
     regulatory      complement(5994..6110)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1753 a         1266 c          981 g         2114 t
ORIGIN      
        1 tatattacta tacattatcc gttacaggtg gaacagaatt ttttacatct acaaaaaaaa
       61 tgcttcttgt aaaataattt taaccgtttc ggctgagtca agtgtttttc tgactttaga
      121 gatataaaaa taattttatt gtcacctcaa agttacttaa ttttcggact ccgcggaata
      181 agcagcaatt tattttaccc caataccggc aatttgtttt cagacactga ggaataagaa
      241 tttaccttct tttataaaaa gtttcagaaa ttcaatccgt attttcttct tctctgttat
      301 tatttattat tatcaatcac ataaaacttt tatacctatc tggtttacag cttcttttca
      361 tttgaactga tttttagatt taaatataaa ctcatttaat aaattcacca aaatatttaa
      421 attgagatat gctagttcct aaattattta caactctaaa aacttataat aaaaaacaat
      481 tttataaaga tgtcattgca ggaataattg tcgggataat tgcacttcct ctggcaattg
      541 catttggtat tgcttcaggt gtatctcctg aaaaaggtct tattactgct gtaattggtg
      601 gatttttaat ttcagtatta ggtggaagca gggttcagat cggcggtccg acaggagcat
      661 ttattgtaat tgtttacgga attgttcaca actacggact tgacggtttg atgatagcta
      721 caataatggc aggtgtcata cttataatta tgggatttat gaaacttggt aatcttataa
      781 aattcatacc aattcctctc atcgccggat ttacttccgg aatcgcgctt ataatattta
      841 caacagaatt aaaagatcta tttggtttaa aaattgaaaa tctccctgca gaattcctgg
      901 aaaaaatccc tgtatatatt caaaatttta atacaataaa tactgatact gctataatat
      961 cactgatcac attactaata attatttttc tgccttatgt tacaagaaag atccctgcaa
     1021 tatttcttgc tctggtagtt ggaactttgg ttacacattt ttttggattt aatgtagaaa
     1081 ctataagttc aagattcggt gaaatttcca attcaatacc atctccttca tttcccgttt
     1141 tctcttatac aaaagtttat gctttacttg ggccggcttt tacaattgcc atacttgcag
     1201 caattgaatc tttacttagt gctgttataa cagacggtat gaccggagga aggcatcgat
     1261 caaatatgga actcatcgcc aacggtgcgg caaatattgc ttcaccaatt tttggaggaa
     1321 taccagtgac aggtgcaatt gccagaactg tgacgaatat taaaagtggc ggtagtacac
     1381 cagttgccgg aattattcat tcattaattc tttttcttgt cataattttt tttgggaaat
     1441 atgccggtat ggtgccgctt agtgtactgg ctgcaattct tgttgctgta gcttataata
     1501 tgttcgactg gagagaattc agatcacagc taaagatgcc taaaagtgat gtgatcgtat
     1561 tcctctctac tttttttctg acaatattat ttgacttaac tattgcaatc gagatcggga
     1621 tgatactgtc ggcattttta tttatgaatc ggatggcaaa ggtagctaac attggaatta
     1681 ttaccagaga gttaaatgat gattctgata cagaagatgc tgaaataaat tctattcaaa
     1741 acaaaaatat tccggatggt gttgtgatat atgaagtaaa tggtcctttc tttttcggtg
     1801 ctgccggtaa attcaatgat gttttaagga caattcattc taaatccaaa atattgatct
     1861 tacgattgag aaatgttcca gcaatcgatg ctacaggaat caatactata gaaaatattt
     1921 atgaagattg caggaataaa aaatctgtgc tgatcctgtc aggtattcac gctcagcctt
     1981 tgtttgctgc agagaaagcc ggacttatag aaaaaatcgg aattgaaaat gtaaaaggta
     2041 atatagaaga tgctctgaac cgggcaaagg aaatattagg aattcctata gatacaatga
     2101 atcaggatga tgaaagttct gaaggtaaat aattttgatt ttataacgta cccgcagagt
     2161 cacgtgattt ccagactctg cggagacaag aattattaat cataattcaa aaccggcgat
     2221 aaccatctct ctacttcctc aatagtcatc aactttctct ttgcataatc ttccgcctga
     2281 tctttgctga tctttccaag tccaaaatat tttgattcgg gatgtgcata atacatacca
     2341 ctgaccgatg aagccggata cattgcaaga ctttctgtaa gttttatacc tgtatttttt
     2401 tctgcatcaa gtaattcaaa caacgtccac ttctctgtat gatcaggaca tgctggatag
     2461 cccggtgctg gtctgatgcc tcggtatttt tcagtaatga gatcttcatt ttcaagtttt
     2521 tcatttttat catatcccca gtattcagtc ctcacttttt tatgcagcat ttcagcgaac
     2581 gcttcagcaa gtctgtctcc cagtgcctgc atcataattt tattataatc atcatgatct
     2641 tcattatatt tttttatcca ttcttctatt cccatacctg cagtaactgc aaacccacca
     2701 atataatctg taagtcctga ttcttttgga gctataaaat ctgacaaagc taagttcggc
     2761 tgacctttac ttttttcatt ttgttgtcga agagtgtgaa ttgttttcag tacttcagtt
     2821 ttactttcat ctgtataaac ttcaatatca tcaccgacag aattcgcagg aaaaattccg
     2881 aatactgcat ttgctgtaag tcttttttct ttgatgataa tattcaaaag attctttgca
     2941 tccagaaata aattcgcagc ctgctcaccg acaaccttgt cttctaaaat ttcaggatat
     3001 ttccccgcca gctcccacgt cctgaaaaac ggcgtccagt ctatgtattc agcgatctcc
     3061 tgtagaggat aatcatttaa aatttttata cctttaaatt caggtacagc tatatcttct
     3121 tttttgaaat caatttcaaa tttatttttc tttgcatctt catattttaa ataatttttc
     3181 tgaactttat ttttcaaatg atgctcacgg agttttgtat attcatcttt tattccggac
     3241 tcaaaagctc ctttattttc attaagtaaa tttccgacaa caggcactga cctcgaagcg
     3301 tcaagtacat ggatcgtctg acctcttttg taattctgtt cgatctttac cgctgtatga
     3361 actttagatg tagtcgctcc tccgatcaat agcggaatgt caaatccttc gcgttccatc
     3421 tcctttgcaa cgtgaaccat ctcatcaaga gacggagtaa taagtccgct aagtccgatg
     3481 atatcagctt tttcatttct cgctgcttca agtattttct cacaaggcac catcacgcca
     3541 agatcaacga catcataatt attacagcca agtacaactc ctacaatatt ttttccgatg
     3601 tcatgcacgt cgccttttac agttgccatc agaattttac ctttaggttt tgaatcaact
     3661 gtcttctcag cttcgatata cggcagcaaa tatgctacag cttttttcat cacccgtgca
     3721 ctttttacca cctgcggcaa aaacattttc ccctcaccaa aaagatcacc gactacattc
     3781 attccgtcca ttagcggacc ttcgatcact tcaattggct tatcaaacat ttgcctcgcc
     3841 tcttctgtgt cttcgactat gtaatctaca attcctttga caagtgaatg agtaagtctt
     3901 ttcttcacat ccatctttct ccatgtttca tctttgacct gaactttccc tttatgtttt
     3961 atattttccg caaattcaac aagcctttca gttgaatcag gtcttctgtt aagtaaaaca
     4021 tcttccactc tttccaggag atcttttgga atttcttcat acacttctat catacccgga
     4081 tttacaattc ccatatccat acctgctttg attgcatgat acagaaatgc tgaatgcata
     4141 gcttccctca ctacattatt tcctctgaat gaaaatgaaa tattactcac accaccgctc
     4201 acctgagcac cgggcagatt ttcctttatc cattttgtcg cttggatata atcgactgca
     4261 taattattat gctcttctat tccagtagca actgttaaaa tattcggatc aaaaataata
     4321 tcagacggat tgaagccgac cttctttgta agaatgtcat aagctctctg acatatctca
     4381 atttttcttt cataagaatc tgcctgacca gtctcatcaa atgccatgac aatcactgct
     4441 gctccataca tccttacctt gcgtgcactt tctatgaact tctcttcacc ttctttcatt
     4501 gaaatggaat tgacaattgc ctttccctga acgcatttca aaccggcttc aatgacagac
     4561 cacttagatg aatcgatcat gataggaagt tttgcaatgt ccggctctga accgatcaga
     4621 tgtaaaaatt ttgtcatcac ctcttcagag tcaagcatac cttcatccat attcacatct
     4681 atcacctgag caccgccttc tacctgatgt cttgcaatgg caagtgcgtc ttcatatttt
     4741 tcatcgatta ttaatttttt aaactgtgct gaacctgtaa cattcgtccg ctcgcctaca
     4801 ttcacaaaat tacttccttt gtaaacttca agcggctcca gaccactgaa tctcggaatg
     4861 tctgtgatca ccggcggaac tcgcggcttg aaattttttg tgacttcata gatcgcatta
     4921 atatgctcag gcgttgtacc gcagcatccg cctacaatat tcaatagacc ctcatttaaa
     4981 aactcttcta tctgtttagc agttgattca ggtgtttcat catattcgcc gaactcattc
     5041 ggaagacctg cattgggatg agcgctgata agcagatcag atttttctga gatagtagag
     5101 atgtgcggtc gaagctgctt tgcgcccagt gcacaattaa atcctatact taaaagttca
     5161 agatgattta ctgaatacag aaatgcttca acagtctgtc cagacaaagt ccttccgctc
     5221 gcatcagtaa ttgtacctga caccatcaca ggaattttct ttcctgtatc ttcaaaaagt
     5281 ttttctatgg caaacaaagc tgccttggca ttgagtgtat caaagatagt ttcaactaat
     5341 aagatatcga ctcctccgtc catcagtcct tttgcctgtt cataatatgc ttccatcagc
     5401 tgatcaaaag taacagctct gaatccggga tcatttacat caggtgaaag tgaagctgtc
     5461 ctgtttgtcg gacccattga accggcaaca aacctcggtt tttcgggctc attttttgta
     5521 aattcatctg caacttcttt tgcaagtttc gctgattcaa agttaagctc atatacaata
     5581 tgctcaagtt tataatccgc ctgtgcgatc gtagtcgagc tgaatgtatt tgtctcagca
     5641 atgtctgcac ctgctttgaa atattcggta tgtatctctt ttataatatc cggtcgggta
     5701 atagacaaaa gatcattgtt gcctttcagc ggatgagaat gatctttgaa tctttcacct
     5761 ctgaagtctt cttcttcaag tatgtgcctt tgtatcatag ttcccattgc gccgtcaaga
     5821 actagtattc tgttctttaa aatttcttta atgtcattca ttcaaatttt tttgattatt
     5881 aaattcgtaa atcttgttgt cttataatta tatgattaaa aaaaaaatct cctcaggcaa
     5941 tagccggaag agatttcaaa atttatattg gtatataatg tttgcaatcc tccgactcat
     6001 cttgtctccg gaaatcccgg agtaggaatt ggcacctttc gttcccgttt tcaggaagac
     6061 ggttgccaag gtatcatagg gcctaatccc tccacctttc tccataagtc agat
//
LOCUS       DAORVQ010000197         6113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8373_length_6113_cov_6.367613, whole genome shotgun sequence.
ACCESSION   DAORVQ010000197 DAORVQ010000000
VERSION     DAORVQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8373_length_6113_cov_6.367613"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1232
                     /locus_tag="PLX80_12425"
     CDS             366..1232
                     /locus_tag="PLX80_12425"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY21639.1"
                     /translation="MRKKISVISKLLFISALALIWFGSRSGNVSSDSSSPEGYKYGQV
                     VWRDLVTPNPKAAAEFYNKVFGWTSSQVGTDEQPYWVFKSNGKPVGGMHLMSEARKGA
                     GGEWVQYYSVSSLEDVIKKSKTAGGELVVSPLEVEGRGTVALISDPQKAYFALLKPEK
                     GNFSDAKPMDYEFLWNELWSNDIDKSVEFYKSTFGSTTETKEDDDRPYTLLLNNNQPI
                     SGIIKNPVDNVRSHWMQYVKVSDVKSIEQKAIDAGAKMIIKSDSTVRKGTVSIFTDPT
                     GAPIAIQKWPIE"
     gene            1304..1492
                     /locus_tag="PLX80_12430"
     CDS             1304..1492
                     /locus_tag="PLX80_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21640.1"
                     /translation="MNKIILYILIAITAFTVYSCAGVTVSSGVGYGMYGGPYGYGTYG
                     YSPRVNVGMYGGGFRYYP"
     gene            1787..2203
                     /locus_tag="PLX80_12435"
     CDS             1787..2203
                     /locus_tag="PLX80_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY21641.1"
                     /translation="MSKKMCPVVHFEMPFEDKSRAIDFYEKVFGWRSQMLGPEMGNYV
                     VVSTSETDEKTHMPKEPGKIGGGLFEKTENNKSTSVVIAVEDIQAAMKNVADAGGEVL
                     GGMNPGEPDDIPGVGLYCAIKDSEGNLVGILQPKGM"
     gene            2394..2915
                     /locus_tag="PLX80_12440"
     CDS             2394..2915
                     /locus_tag="PLX80_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21642.1"
                     /translation="MKTLSCFIIALTFFIFSENSYSQSWLPKPVESVYADALTGTWTS
                     EPYEFMGNTNTDVVTCSKILNSQYLEIDFKRTDNTGFTYEGKEIVSVSPDGNMNGMYY
                     DIMGKEKALSYTGSKDGDKLILMGISPVGKGSREIIIDGNTMTHNVKFVMSDDADSSA
                     PEQNIKIIYNKTN"
     gene            3181..4200
                     /locus_tag="PLX80_12445"
     CDS             3181..4200
                     /locus_tag="PLX80_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21643.1"
                     /translation="MKSLIILFLSLLLISCGGSDDNESNLASQAETIYAPAERTDDDD
                     DLDNDNVMMRGNCTPPNLKLDVNINRKYCKTDDCLIEFAGYWWWTNYQYTGSPNWFWN
                     QGQAWSPGNASVDSEGLHLRVKKDDLGGGLDWMASEVVAVYKSDKKTLAGTGYGTYLV
                     SARVKSADSWDKLDKNVAFGLFTFQNDRTGPVNNPYREIDLAEISRWGTPPNSNVSDT
                     RLYTGNAQFAIQEWDKNQKNLHRYTISNNVKEITLVMKWTNEKTPVTFSQYNGIYTLD
                     NLPATPNNTFTTAADQNQYVPADGCQLFHMNLWMGNYGQGNKHPGPSNNQVQEVVVTN
                     FQYKP"
     gene            4628..5785
                     /locus_tag="PLX80_12450"
     CDS             4628..5785
                     /locus_tag="PLX80_12450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY21644.1"
                     /translation="MKKIYINLFVCLIFLNSVNVSGFQKIISSENVSSPVPFSDLETL
                     LDNTVAQTFQDLKIPGAIIGVWKGNEKYIKTFGVSDLETGTPIKADDNMRIGSITKTF
                     TGTVVLQLFDEGKLNLSDKLSLYFPDYPNGDSITITMLGNMTSGIYNYSDDEHFVKEL
                     INNLNRTFTPDELIDIAKSHPPYFSPGTGFHYSNSNTVLLGMIVEKITGNSLADEIRS
                     RILIPLDMTKTIFSNDTYFPEPHANGYIYLDSLQTSPTDVTIQNPGWGWAAGAIISNL
                     EDVHKYAKKVADGSLISRNAQTDREKWGEVFIPTSGAWKGKDLRYGFALADFEGAIGH
                     NGGIPGFNSFMGYIPDSDITIIILCNMQDNLEGIGPADFLAMKIVETFKNN"
BASE COUNT         2112 a          982 c         1151 g         1868 t
ORIGIN      
        1 taaagaaata tttgagcaaa ttaaaataat ggaaacaata aacaggcgga tcagcatctg
       61 aatatttaat ttataaaaaa ttgtttatca aaatatattt atcgtctgtc taattaaagt
      121 taataatgtg aggttttaat tctaattttg aatattgcct caaagactcc aagtgaataa
      181 caataaatct attatctgaa tgatgaatgg agtgagtttt taattttttt taactttgag
      241 gcttagtgtc ttagaggtaa ctatattcta ttttgttaaa tccgacatct aagataaatt
      301 tttataattt tataataaga ttattattgt attttagatt taaaatcaaa aataaaatca
      361 gaaatatgag aaaaaagatt tcagtaattt caaaattatt atttatatca gcactggcat
      421 tgatctggtt tggcagccgg tcgggaaatg tttcttcaga cagttcatct ccggagggat
      481 ataaatacgg gcaggtagta tggagagatc tcgtcacgcc aaatccaaaa gcagcagcag
      541 aattttataa taaagtattc ggatggacct cgtcgcaggt cggcactgat gagcagccat
      601 actgggtttt taaaagcaac ggaaaaccag ttggcggaat gcatctgatg tcagaagcaa
      661 gaaaaggagc tggcggagaa tgggtgcagt attattctgt gagttcactc gaagatgtaa
      721 tcaaaaaatc aaagacagca ggaggagaat tggtagtgtc accgttggaa gtggaaggac
      781 gcggaacagt tgcacttatt tcagatccgc agaaagcata ttttgcattg ttaaaacctg
      841 agaaaggaaa tttcagtgat gcaaaaccaa tggattatga atttctctgg aatgaactct
      901 ggtcaaatga tattgataaa tcggttgaat tttataagtc aactttcgga tcaacgacag
      961 agacgaaaga agatgatgac agaccatata ctttgctcct taataataat cagccgattt
     1021 caggaataat aaaaaacccc gttgacaatg tcagaagcca ctggatgcag tacgtgaaag
     1081 tctctgatgt aaaatctatt gagcagaaag caattgacgc aggagcaaaa atgataatca
     1141 aatcagactc taccgtcaga aaaggtactg tatcgatctt taccgatccg accggtgcac
     1201 cgatcgcaat tcagaaatgg ccgatcgaat aaataacaga atataatttc tcagataatt
     1261 gatcagaacg atttactgaa taaaaaattt taacaataaa aaaatgaaca aaataatttt
     1321 atatatttta attgcaataa cagcttttac agtctattca tgcgcaggag ttacggttag
     1381 ttcgggagtg ggatatggaa tgtacggcgg accatacgga tacggcacat atggttattc
     1441 accccgggtt aatgttggca tgtacggtgg tgggttcagg tattatccgt gatgattaaa
     1501 tttaaaaaaa agatttttaa taatttttgt acagcaacta tataagttta ccgtgtctga
     1561 attaaattta aaattcagac tttaaatctt taggatcttt tttcaaaatt caatcagtca
     1621 taaatttatt attcccttat caaaataaat ttgtcgcatt aaacccctaa taatggcgta
     1681 aatgcatttt gaataataat caaaataatg gtatctttgt aaaagctgct gaatgaattt
     1741 aaagtaatca tcagcggaat taaaaaaatt taaataaata attatcatga gtaaaaaaat
     1801 gtgtcccgta gttcattttg aaatgccatt tgaagacaaa agccgtgcaa ttgatttcta
     1861 tgaaaaggta ttcggatgga gatcgcagat gctcggtcct gaaatgggga attatgtggt
     1921 cgtgtcaact tctgaaactg atgaaaaaac tcacatgcca aaagagcccg gtaagatagg
     1981 cggcggttta tttgaaaaga ctgaaaacaa taaaagtact tccgttgtga ttgctgtcga
     2041 agatattcag gctgccatga aaaatgtagc cgatgcaggc ggtgaagtat taggaggtat
     2101 gaatcccgga gagcctgatg atattccggg agtcggactt tactgtgcaa ttaaagacag
     2161 tgaaggtaat ctcgtcggaa ttcttcagcc aaaagggatg taaatttttt tttaaatttt
     2221 atttttactt tagacgtcag gatattttgt tctgacgttt ttaatttata cttatcagtt
     2281 taatattaat atatacacat tatgaaaaga gttctcttta ataatttaaa caattgaatc
     2341 tcattttcta aatcaataat tttgctcatt cataaaatta ataaaaatca ttaatgaaaa
     2401 ctctctcctg ttttattatt gctcttactt tttttatttt ttcagaaaat tcttattctc
     2461 agtcttggct tccaaaaccg gtcgaatcgg tttatgctga tgccctgacc ggtacatgga
     2521 cctcagagcc ttacgagttc atgggaaata ctaacacaga tgttgtaact tgcagcaaaa
     2581 ttctgaacag tcagtatctt gagatcgatt ttaaaagaac tgataacacc ggattcacat
     2641 atgaaggaaa agaaattgtt tctgttagcc cagacggaaa tatgaatgga atgtattacg
     2701 acattatggg aaaagaaaag gctttatctt ataccggttc aaaagacgga gataagctga
     2761 tactcatggg tataagtcct gtaggtaaag gttcacgtga aataatcatt gacggaaaca
     2821 caatgactca caatgttaaa ttcgtaatgt ctgatgatgc agattcatca gcacccgaac
     2881 aaaatattaa gatcatttat aataaaacga attaatattt tttaatttat tgtgatctgt
     2941 tttttgcaaa ttttaagcgg cagtaaatta ttaaaaagct cactttcaac ttaaataatt
     3001 tttttaaata agacgtcagg aatattcttt ctgacgtttt tatctttata aatccatcct
     3061 gattagctga cttcagttat tgaaaagata tatcgtctag aatttaaaca attgaatttt
     3121 attttctaaa tttatacttt tgctcacccc catattattt aaaattaaat taaaactctc
     3181 atgaaaagtc ttatcatttt atttttatca ctattattaa tttcgtgcgg cggatcggat
     3241 gataatgaat cgaacttggc atctcaggct gaaaccattt atgcaccggc tgagagaaca
     3301 gatgacgatg atgacttaga taatgataat gtaatgatga gaggaaactg tacaccgccg
     3361 aatttaaaac tcgatgtcaa tatcaacaga aaatactgca aaacagatga ctgtctgata
     3421 gaatttgcag gatattggtg gtggacaaat tatcaataca ccggatcacc aaactggttc
     3481 tggaatcagg ggcaggcatg gagtccgggc aatgcatcag ttgacagtga aggtcttcac
     3541 ctgagagtaa aaaaagatga cctcggaggc ggactggact ggatggcgtc tgaagtagtt
     3601 gcagtttata aaagcgataa gaaaactctt gcaggcacag gatacggaac ctaccttgtt
     3661 tcagcccgcg taaaaagtgc tgattcatgg gataagcttg ataagaatgt tgcattcgga
     3721 ttgtttactt ttcaaaatga cagaaccggt ccggtaaaca atccatacag agagatcgac
     3781 cttgcggaga tttcaagatg gggcacaccg ccaaacagca atgtcagcga cacaagactt
     3841 tatacaggaa atgcccagtt cgcaattcag gaatgggata aaaatcagaa gaaccttcac
     3901 agatatacca tcagcaacaa tgtaaaagaa attacgctgg ttatgaaatg gactaatgaa
     3961 aaaacgccgg ttacattctc acagtacaac ggaatttata cactggataa tcttcccgca
     4021 acacctaata atacattcac gactgccgcc gatcagaacc agtacgtacc tgcagacggc
     4081 tgtcagcttt ttcatatgaa tctgtggatg ggaaattatg gtcagggaaa taaacatccg
     4141 ggaccgagca ataatcaggt gcaggaagtg gtggtgacta attttcaata taaaccctga
     4201 tcaatattta atattaaatt ttaaatatta aatgtttgga gctgaaataa ttaactggct
     4261 ttgtaagacc ttactaattt tataatagca gttgatctcg tttgagccga tgttgatgtt
     4321 taaaaagttt gcgaataaaa aagtgtgttt tgaattaaat aactatgcaa acttttttat
     4381 caccaacaat cgtgaatgtt caattataaa tgttcaatgt gcagtgattt tcccgggacg
     4441 gcgaaatatt ttgagccccg gttaagtttc tataagtttg cgaataaata agtatgattt
     4501 gaatttaaaa cctgtgtaaa ctatttgatc agatattgca aatgtccaat gatcttcata
     4561 tcggagtctc cggtaaattt aaatcaataa aacaatttca tgtttattat aacttttaaa
     4621 ttatttgatg aaaaaaatat atataaatct atttgtctgc ctgatctttt taaattctgt
     4681 aaatgtatcc ggatttcaga aaataattag cagtgaaaat gtaagttccc cggttccatt
     4741 cagtgatctt gaaactttgt tagacaatac agtcgctcag acatttcagg atctgaagat
     4801 ccccggtgct ataataggtg tctggaaagg taatgaaaaa tatataaaga cctttggagt
     4861 ttcagatctt gaaaccggta cgccgattaa agcagatgac aacatgagaa tcggcagcat
     4921 tacaaagacc tttaccggta ctgtggtgct tcagcttttt gacgaaggaa aattaaatct
     4981 tagcgataag ctttcactgt attttcctga ctatccaaac ggagacagta ttacaataac
     5041 gatgctcgga aatatgacaa gcggaattta taattattcc gatgatgaac attttgtaaa
     5101 ggaattgatt aataatttaa acagaacctt tacaccggat gaattaatag atatcgcaaa
     5161 atcccaccct ccatattttt caccgggtac aggttttcat tattctaatt ccaatactgt
     5221 attgcttgga atgatcgttg aaaagataac gggtaatagt cttgcagatg agatccgcag
     5281 cagaatttta attccgcttg atatgacgaa aaccattttt tcaaatgata cttattttcc
     5341 tgaacctcac gcaaacggtt atatatattt ggattccctt cagacatcac caacagatgt
     5401 aacaatacaa aatccaggct ggggttgggc tgccggagct atcatttcga accttgaaga
     5461 tgttcataag tatgcaaaaa aagttgctga tggaagtctt atcagcagaa atgcccagac
     5521 agacagggaa aaatggggag aggtattcat tcccacatca ggtgcctgga aaggaaaaga
     5581 tcttagatac ggatttgcac ttgcggattt tgaaggtgcg ataggacata acggaggaat
     5641 acctggcttt aacagtttta tgggttacat acccgattct gatattacaa taattatact
     5701 atgtaatatg caggacaact tggaaggaat aggaccggct gattttcttg caatgaaaat
     5761 agtggaaact tttaagaata attaagaaac tttatttgag ctgttgttga tgtttaaaaa
     5821 agtttgcaaa taaataagta ttacttgaat ttaaatccac tacaaacttt ctgtacacca
     5881 acaatttcga atgttcaata ttaaatgttc catgtgcaat gattttctat cggcagcata
     5941 atcttaatat aatattttaa aacaatatat gaacttaaag aagtctattc aatcttttcg
     6001 gactcagacg atttcaccag cgtcagcttg caacaacccc ctccgctccc ccttttttga
     6061 gactgtctca aaacatctgt tcttaccact gagacaccaa gacacaaagg aaa
//
LOCUS       DAORVQ010000198         6110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8381_length_6110_cov_5.300908, whole genome shotgun sequence.
ACCESSION   DAORVQ010000198 DAORVQ010000000
VERSION     DAORVQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8381_length_6110_cov_5.300908"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..1855)
                     /locus_tag="PLX80_12455"
     CDS             complement(344..1855)
                     /locus_tag="PLX80_12455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21645.1"
                     /translation="MKKVYFGITAAVIIIAMTGFDKFKPFDNVSIIDLKKIDANQIST
                     FVRNNGSFDRQPNTGNAGFEWPKGAGTYAVYASGLWISCKVNDSIRVSLADYNSEFRP
                     GYFDHNTQMPMGADDPDYRLYKVSPAFPNGSIDFDAWNLWPVQQGAPWEDNNGNGIYE
                     PPADNPVMKGDQNLFCSFTDGYRDSIQNIRSTPPLRAEIHLYAWAKTQSVCADVINYE
                     WKIINKNISEWTEFSSAIWSDIDIGTSNNDKAGSDSAKNLAYGYNATNNDPVYGLAPP
                     AVGVIIKEATGHENSQHKSDYGYVFKCSIGDCPGDSLEVYRVMHGLRTDGSEIINPVT
                     NKVTRYSYSGDPLNGSGWNDSTQGDRYIFIGSMIGDVNSLDTVDFKTISIIKKGTSNL
                     ASINELKHCVDLVISVNNISSEIPGDFKLYQNYPNPFNPLTKIRFSINNKNFVTMKIF
                     DVLGREIKTLVSSNYDTGTYEVNFNAEGLSSGIYYYTLKAGDFIDTKKMMVVK"
     gene            complement(2198..2377)
                     /locus_tag="PLX80_12460"
     CDS             complement(2198..2377)
                     /locus_tag="PLX80_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21646.1"
                     /translation="MELPQRLKDSKAAISKTIKMKFFDPYKLRGKLIIFVIGITSKTQ
                     RLQGCNFENNKNEIP"
     gene            2518..2790
                     /locus_tag="PLX80_12465"
     CDS             2518..2790
                     /locus_tag="PLX80_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQY21647.1"
                     /translation="MLSLLWSAIIGLITGALAKLVLPGKDPGGFFVTMLIGVAGSLLF
                     TFLGRMIGWYSEGESGGFIAGFLGAVVLLLIYRMVRGKSAARAVKD"
     gene            2835..3248
                     /locus_tag="PLX80_12470"
     CDS             2835..3248
                     /locus_tag="PLX80_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQY21648.1"
                     /translation="MALQEKYQSVLDTINQSGGVDVSVNEDNGVLNITATVPSPDEKV
                     KVWNKIKEVGGDNPTDLMADIRMMNSEVASGPVGGSGEGVGQRTYTVRSGDSLSKIAK
                     EMYGDGNKYMDIFNANKDKLSDPDAIQPGQELIIP"
     gene            complement(3492..3725)
                     /locus_tag="PLX80_12475"
     CDS             complement(3492..3725)
                     /locus_tag="PLX80_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21649.1"
                     /translation="MIIGTNRAIGKVFNSCIRPLIFTEEMGTAWLKHNIEEAGNFEVF
                     YRNCIIKKSRNEETAERMTERNIACNTGFASTG"
     gene            complement(3784..4362)
                     /locus_tag="PLX80_12480"
     CDS             complement(3784..4362)
                     /locus_tag="PLX80_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQY21650.1"
                     /translation="MTKLNTIPVKEFKTAESFETWLQKNHNNSKGLWLKIFKKNSGEK
                     TVSYAEAIDAALCYGWIDGQRKAYDEQAYLQKFCPRREKSIWSKINTGHVARLINEGR
                     MRTSGLKAVEKAKTDGNWERAYDPPSKMTIPEDFLKELGKNKKAKAFFTGLNKSNLFS
                     IGFRLQTAKKQETREKRIKEIIEKLANGEKFN"
     gene            complement(4665..5033)
                     /locus_tag="PLX80_12485"
     CDS             complement(4665..5033)
                     /locus_tag="PLX80_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY21651.1"
                     /translation="MTTHFENLIKKAYPAFNSRDIDTALLTFHSNVQWPKAFEGGYVN
                     GHDEIREYWTRQWTEIDPTVEPTGFNQRPNGTFEVTVRQIVKDLKGNLIFDGEVKHIY
                     TLEDDLLRRMDIEIDRTEFS"
     gene            complement(5253..5636)
                     /locus_tag="PLX80_12490"
     CDS             complement(5253..5636)
                     /locus_tag="PLX80_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HQY21652.1"
                     /translation="MIPIFSLVFLISESYSKLDKHIQSNKARLLFSAELYNEFLDVVS
                     RPKFKKFFAEIDLNKLTESINEHADFIDVTAQVDICRDKKDNFLPALCEDGKADYLLT
                     GDEDLLVLKKFKGTSIIKIAEYLRK"
     gene            complement(5654..5863)
                     /locus_tag="PLX80_12495"
     CDS             complement(5654..5863)
                     /locus_tag="PLX80_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21653.1"
                     /translation="MTTFNIEILDPKGKKLLMDLAELRLISITENTANQFSDTVNKIR
                     SKKAKLSLSDITKEVESIRSKRNAG"
BASE COUNT         1753 a         1110 c         1061 g         2186 t
ORIGIN      
        1 aatttatcac ccggaaaata tgaagctgat tttgatgcag gaaatttacc aagcggtgtg
       61 tatttttaca gacttgaagt gagcgggaca aatcaattta cttcaggaaa gttttcgcag
      121 acaaagagta tgatactcct caaataattt taaattttaa attttaaatt ttaaatagga
      181 ttacttgagt tgattaatag tccgtaaagt gacattattt ttggagacca aaaaagtttg
      241 atgtacttct aaatttatac agcacattag atcttcaaac ttttttggaa ctccaacaac
      301 ggcgggatat gaaaatttca aatttaaaat ttaaaattta aaattatttc accaccatca
      361 tcttctttgt atcaataaaa tcacccgcct ttaaagtata ataataaatt cccgaagaca
      421 atccctctgc attaaaattt acttcataag ttccggtatc atagttactg ctgaccaatg
      481 tttttatttc cctgccgagc acatcaaata ttttcatagt cacaaaattt ttattgttaa
      541 ttgagaatct gatctttgtt aaaggattaa atggattcgg ataattctga tacaatttaa
      601 aatctccggg tatttctgaa ctgatattat taacagaaat aaccagatcg acacagtgtt
      661 taagttcgtt tatacttgcc agattacttg ttcctttttt aattatagaa attgttttaa
      721 aatctacggt atcaagagaa ttcacatccc cgatcattga acctataaaa atatatctgt
      781 caccctgagt ggaatcattc catccgcttc cgttcagggg atctcctgaa taactgtatc
      841 ttgtaacttt gtttgtaaca ggattaatta tttcacttcc gtcagttctg agaccgtgca
      901 tgactctgta aacttcaagc gaatctcccg gacagtcacc tatactgcat ttgaaaacat
      961 atccgtaatc agatttatgc tgactattct catgaccggt agcttctttt atgatcacgc
     1021 ctacggcagg aggtgcaaga ccataaacag gatcgttatt tgttgcgtta tatccgtagg
     1081 caagattttt tgcagagtct gatcctgcct tgtcattgtt tgatgtacct atgtcaatat
     1141 ccgaccagat agccgaactg aattccgtcc attccgaaat attcttattg ataattttcc
     1201 attcgtaatt tattacatca gcgcagactg actgggtctt tgcccatgca tataaatgaa
     1261 tttccgctct aagtggagga gtacttctta tattctgaat tgaatccctg tagccgtcag
     1321 taaacgaaca gaaaagattc tgatcacctt tcattacagg attgtctgcc ggcggctcat
     1381 agattccgtt gccgttattg tcttcccatg gtgctccctg ctgtacaggc cagagattcc
     1441 atgcatcgaa atcaatgctg ccgtttggaa atgccggcga tactttgtat aatctgtaat
     1501 caggatcatc tgcacccatc ggcatctgcg tattgtgatc aaaatatccg ggacggaatt
     1561 cagaattata atccgcgagg cttacgcgga tagaatcatt taccttacag ctgatccaga
     1621 gcccgcttgc atatactgca taggttcctg ctcctttcgg ccattcaaat cctgcattac
     1681 cggtattcgg ctgtctgtca aaactgccat tgtttcttac gaatgtactg atctgattag
     1741 cgtcaatttt ttttagatct attatagata cattatcgaa aggtttgaat ttatcaaaac
     1801 ccgtcatcgc aataattata actgctgcag ttattccgaa ataaactttt ttcattttca
     1861 aaacagatct ccttaaaatt tattttttag ttatacaatt ataattattt ttttaagaaa
     1921 taccaaagaa atatatttga gtttgtgcgg atgtttattt tttataatat atgatttttt
     1981 atgcaattga tttgttttct gcttgaaatt ccattttttc ttcggaacga aaacgctgtt
     2041 tgtgtgaaag ttcatgcctc aaagtctcta aggatcaaag gaaataattt taatcttata
     2101 aaacttaaaa acttaaaaac ttaaaaactt aaaaacctgg agacttggaa caattccatt
     2161 cttattaata ataaatttgg ttcaaagtct ctatggctta aggaatttca tttttattgt
     2221 tttcgaaatt gcagccttgg agtctttgag tctttgaggt aattccaatt acaaaaatta
     2281 taagtttgcc tctaagttta taaggatcaa agaatttcat ttttattgtt ttcgaaattg
     2341 cagccttgga gtctttgagc ctttgaggta attccaattt aatgttattg gaatttgagt
     2401 ctttgaggta attccaaaga tataaactga gttttgtcct ctaataattt tatacaaatt
     2461 aataatgata atacttatat tagctttata ttttttaaat taatcaaagg agctcacatg
     2521 ttatcacttt tatggtctgc aataatcggt ttaataaccg gtgctttagc aaaactagta
     2581 cttcccggaa aggatcccgg agggtttttt gtaacaatgc tgatcggagt agcaggttca
     2641 ttacttttta catttttagg aagaatgatc ggatggtatt ctgaaggtga gtccggcgga
     2701 tttatagcgg gattcctggg agctgtcgta cttcttttaa tttacagaat ggtaagaggc
     2761 aagtctgcag caagggcagt caaagattaa taaacagcaa aagattttta caaaataaaa
     2821 tacaggagat aaaaatggct ttacaggaaa agtatcaatc agttttggat acaataaatc
     2881 aatcaggcgg cgtggatgta agcgtcaatg aagataatgg tgtattgaat attactgcta
     2941 cagtcccgtc accggatgag aaagtaaaag tatggaataa gatcaaggaa gtaggcggtg
     3001 ataatccgac tgacttaatg gctgatatca gaatgatgaa ttctgaagta gcatcaggtc
     3061 ccgtgggcgg cagcggagaa ggtgtcggtc agagaactta cacagtccgg tcaggtgaca
     3121 gcttaagtaa aattgccaaa gaaatgtacg gagacggaaa taagtatatg gatatcttta
     3181 acgctaataa agataaactt tcagacccgg atgcaattca acccggtcag gaacttatca
     3241 ttccataaaa aaatattttt attttcacaa taaagatctg tcggattgat ttctgacagg
     3301 tctttttttt attaccgccg tttttggagt tttaaaaaag tttaaaaaat ttagcgggca
     3361 gaataaattt acctgttcat caaactattt tggtctgcat caacaaaaat acacttaggt
     3421 cgtgatcgaa gcgttaaagc aggtattaat tcaaaagttc agtttaaaaa ctacagttga
     3481 atttttgcac gtcaccccgt tgaggcaaat cccgtgttac aggcaatgtt tctttctgtc
     3541 attctttctg cggtttcttc gtttctactt ttctttataa tacaattccg gtaaaagacc
     3601 tcaaaatttc cggcttcttc gatattgtgt ttcagccagg ctgttcccat ttcttctgta
     3661 aatattaaag gtctgataca ggaattgaaa actttaccga ttgctctgtt tgttccaata
     3721 atcaattttg atttacagat agtgttttcc ctcccggata aaatcgtgct gaagtttgat
     3781 catttaatta aatttctcac cgtttgccag cttttcaata atttctttta tgcgcttttc
     3841 tcttgtctcc tgtttttttg cagtctgaag ccgaaatcca atggagaaaa gattgctctt
     3901 attgagaccc gtaaaaaacg cttttgcttt tttgtttttg ccaagttctt ttagaaagtc
     3961 ttccggtata gtcattttgc tggggggatc gtatgccctt tcccaattac cgtctgtttt
     4021 ggctttttcg acagctttta aacctgaggt tctcattctt ccttcattga ttaatcttgc
     4081 aacgtgtccg gtatttattt tagaccagat acttttttct cttcggggac aaaacttttg
     4141 caaataagcc tgttcgtcat atgcttttct ttgaccatcg atccacccat agcaaagagc
     4201 tgcatcaatc gcttctgcat aactgacggt cttttccccg gaatttttct tgaatatttt
     4261 cagccagaga ccttttgagt tgttgtgatt cttttggagc caggtttcaa atgattccgc
     4321 agttttaaat tcttttaccg gaatagtatt taatttagtc atattagttt gtcgtttttt
     4381 cggttctgtg tgcttgccgc tgacgctttg cagatctgcg gtgggcgagc cttttcagcg
     4441 caaatgccgt gcggagaact gcactttcgt ctgccgcaaa actgtctgcg gagcacgaaa
     4501 ccccgcctgc tgcaaatgtg ctgtcagcgg ttcggttttt ttgtcctgcg ttaatatttc
     4561 gctttgtctc cacgtttgta atgaaattga gttttcgtga ttttgtaagg ttgaattaat
     4621 tcctggttgt tctgcttaaa gctgagtatt agctgacttt gagatcagct gaattctgtc
     4681 cggtcaatct caatatccat tcttcttaac aagtcatctt ctaatgtgta gatatgtttt
     4741 acttctccgt caaagattag atttcctttc aggtctttta ctatctggcg gacagttact
     4801 tcaaatgtcc cgtttggtct ttgattaaat ccggttggtt caacagttgg atctatttcc
     4861 gtccattgtc ttgtccaata ttctctaatt tcatcgtgtc cgtttacgta accgccttca
     4921 aaggcttttg gccactgaac gtttgagtga aacgtcaaaa gcgcagtgtc aatgtctctt
     4981 gaattaaagg ccggatatgc ctttttgatt aaattttcaa aatgagttgt cataaataat
     5041 attttaaaat tgttggttta tagttgcttc tgtctggtgt tcgactgtct gtttgtttat
     5101 atgtctttgg ctgccgtttt agcctgaggc tgacatttaa ataccctcaa taaacttaca
     5161 aaaacctgcg catatgtatg ctcctttctt tgtactgaac gcaatcctgt cctaaaataa
     5221 attttcgcct ttccgttatt ttctgattcg ccttacttcc tcagatattc tgcgatttta
     5281 attattgaag ttcctttgaa tttttttaaa acaagaaggt cttcatcccc ggtcaataaa
     5341 tagtctgctt taccgtcttc gcataatgcc ggcaagaaat tatctttttt gtcccggcaa
     5401 atgtcaactt gcgctgttac atctatgaag tccgcatgtt cattaatgct ttcagtcaac
     5461 ttgtttaaat ctatctctgc gaaaaatttt ttaaactttg gtctgcttac tacatcgagg
     5521 aattcattat atagttcagc actaaatagt aacctcgcct tattactttg tatatgcttg
     5581 tccagttttg aataagactc gctgattaaa aaaactaatg aaaatattgg tatcaactac
     5641 aattctctcg cgtttacccg gcattacgtt tcgatctgat actctctact tctttagtta
     5701 tatctgaaag agataattta gcttttttag aacgaatttt attaactgta tcagaaaatt
     5761 gatttgcagt gttttcagta atggaaataa gtcttaactc agcaaggtcc attagcaatt
     5821 tctttccttt tggatcgaga atttctatgt taaatgttgt cataatttta taaaaataat
     5881 tttgtctgaa aaacttgaat ttggatctgt taattttacc ggatttaatt taattaacaa
     5941 atctaactta caaaaactcc cattaatatt caaattagtt tatttcttta gtatgattta
     6001 cgcatcgaaa tttatacaat attttaaacg gttacatggt tttgcacaca gactctttgc
     6061 aaacacttga gtaaaacata tttgtccgaa gcaaaagtta caataaattt
//
LOCUS       DAORVQ010000199         6106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8393_length_6106_cov_5.387209, whole genome shotgun sequence.
ACCESSION   DAORVQ010000199 DAORVQ010000000
VERSION     DAORVQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8393_length_6106_cov_5.387209"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..302
                     /locus_tag="PLX80_12500"
     CDS             66..302
                     /locus_tag="PLX80_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21654.1"
                     /translation="MKHTHILKNAPEVGKVYTVEYDGSELYEAKILDYQGGCWAKIQI
                     ENVLPSPNEKMYKAGQEFDLKLGYYKLFENSESE"
     gene            299..1123
                     /locus_tag="PLX80_12505"
     CDS             299..1123
                     /locus_tag="PLX80_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY21655.1"
                     /translation="MILSKSEIVLNSQQLRFIENGWGKKVTDETEVYNISYDSDGIEV
                     NGYFAKPKNTETKLPLIIWNRGGHENDGRIDEFIAKGMFGEIASWGFTVMASQYRDKD
                     EFGGKDINDILNLMKLAGEFPECDNTKIGMEGWSRGGMMTYKVLTLTDKIKCAVIVSG
                     LADIERNEQDRDGLARAYKKLFGSEDENEFKKRKMERSAVHFYEKINKDTEILLIHGT
                     GDDKVSHQDSLDMYELLKKNKNKCELEIIKGGDHYLTKHRKELVQLRKNWFNKFLK"
     gene            1227..1673
                     /locus_tag="PLX80_12510"
     CDS             1227..1673
                     /locus_tag="PLX80_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HQY21656.1"
                     /translation="MYDPIMVQPMREEVTGIGFRELMSSDEVKTELSKDGTTFIFVNS
                     VCGCAAGKARPGLSIAVEWAKNNNAMPDNLVTVFAGMEKDAVTEARNFFTGFPPSSPQ
                     MAMLKDGEIVHMINRMDIENNDANAVANLIANVFNKYCKKEETEKA"
     gene            1926..4442
                     /locus_tag="PLX80_12515"
     CDS             1926..4442
                     /locus_tag="PLX80_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQY21657.1"
                     /translation="MDSNFSSRVQDVIRLSREEAIRLGHDYIGTEHLLLGIIREGEGI
                     AIKIFKNLGVDAFKIKKTIEDTVRQSGGTLTVGNIPLTKQAEKVLKITYLEAKIFKSD
                     IIGTEHLLLSILREDDNLAAQILHQFNINYEAVKSELMNILSGKPTGNQPGSKASSEK
                     KQDRSKTPVLDNFGRDLTKMAIENKLDPIVGREKEIERVAQVLSRRKKNNPVLIGEPG
                     VGKTAIAEGLALRIINKNVSRVLHNKRVVTLDLAALVAGTKYRGQFEERMKAVMNELE
                     KAKDVILFIDELHTIVGAGGASGSLDASNIFKPALARGDLQCIGATTLNEYRQYIEKD
                     GALDRRFQKIMVDPTTVDETIQILNNIKSKYEEHHNVNYTEAAIIEAVKLSDRYITDR
                     FLPDKAIDVMDEAGSRVHLSNIVVPKEIVDLEAEIEDIRQFKNQVVKNQNYEEAAKLR
                     DNEKKVLADLEQLKLDWDIKSQSMVYEVSEQSVADVVAMMTGIPVNQIAEAESERLVK
                     MEDSLREVVIGQDEAVKNISRAIRRARAGLKDPNRPIGSFVFLGPTGVGKTELAKQLA
                     KFLFNSEDSLIRIDMSEYMEKFNVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVVLLD
                     EIEKAHPDTFNVLLQVLDDGILTDGLGRKVDFKNTIIIMTSNIGTRDLKLDKVFGFGE
                     GTSKTNYEKIRSTIEDSVKRVFSPEFLNRIDDMIIFKQLEKSELQKIVEIGTQKLLSR
                     IELQGLKVEVSKSALDFLSEKGFDKNFGARPLRRAIQKYLEDPLSEEILKGNLKDSGK
                     IKVKHKKGSEELTFTDDKKETASEKESVDITDESKDNDDK"
     gene            4445..5449
                     /locus_tag="PLX80_12520"
     CDS             4445..5449
                     /locus_tag="PLX80_12520"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQY21658.1"
                     /translation="MINKAPYNAMITGVGHYVPERIIGNDYFEEYLDTSDEWIKSRTG
                     IAERRRLAAELPTSYMAIKAIECLLEKRGIGIEEIDLIIVGTVTPDMIFPSTACILQR
                     ELKAVNAWGFDLLAACSGFLFSLATAVQFVQSGSHKKVLVVGADKMSAISNMEDRNTC
                     ILFGDGAGVVLLEPTEDKEYGIVDSILNVDGNGEKYLHMLGGGSLNPPSFETIEKKMH
                     YVYQDGKTVFKEAVKGMADVSYEIMIKNNLTSEQVSYLVPHQANMRIITACAERMGIS
                     MDKVMLNIHKYGNTTSGTIPICISEYWRDGKVKKGDNLILSSFGAGYTWGSILVKWAI
                     "
     gene            5578..>6106
                     /locus_tag="PLX80_12525"
     CDS             5578..>6106
                     /locus_tag="PLX80_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQY21659.1"
                     /translation="MEQITIRSYPKINLGLRILSKRSDGFHDIETIFYPVKIYDEIGF
                     KIEISNTEFNSVILKANKSYIPLNTDNLCYKAVLNFFNEFRIKDPFKIEIDLKKNIPV
                     GGGLGGGSSNAATVLKFLIRYFKIEIESNRKRIIDLALSIGSDVPFFLIMKPSYAEGR
                     GEKIKILNDFRVNHDI"
BASE COUNT         2277 a          917 c         1213 g         1699 t
ORIGIN      
        1 atttccttaa actttgcctt atataaaaga atacaaatta ttaaattatt cctaaattta
       61 tttttatgaa gcatacacac attttaaaaa acgcgccgga agtcggcaaa gtctatactg
      121 tcgaatatga cggcagtgaa ctttacgaag caaaaattct tgattatcag ggcggatgct
      181 gggcaaagat tcagatcgaa aatgtactcc cctctccaaa tgaaaaaatg tataaagccg
      241 gacaggagtt tgatctgaaa ctcggatatt ataagctttt tgagaattct gaatctgaat
      301 gatcttatca aaatctgaga tcgtgcttaa ttcacaacag cttagattta ttgaaaacgg
      361 ctggggtaaa aaagtcactg atgaaactga agtttataat atctcctatg attcggatgg
      421 aattgaagta aatggttatt ttgcaaaacc gaagaatact gagacgaaac ttcctctgat
      481 aatttggaac agaggcggac acgaaaacga cgggagaata gatgagttca ttgcaaaggg
      541 aatgttcgga gaaatagctt catggggatt cacagttatg gcttcacaat acagggacaa
      601 agatgaattc ggaggaaagg atattaatga tattttgaat ctgatgaaac ttgccggtga
      661 attcccggaa tgtgacaata ccaaaatcgg catggaagga tggagcagag gagggatgat
      721 gacttataaa gtattgactc tgactgacaa aataaaatgt gcagttatag tttcaggact
      781 tgctgatatt gaaagaaatg agcaggacag agacggactt gcaagagctt ataaaaaatt
      841 gtttggcagt gaagatgaaa atgaatttaa aaaaagaaaa atggaaagat cagcagttca
      901 tttttatgaa aagataaata aagatacaga gattttgctt atacatggta ccggagatga
      961 caaagtatca catcaggatt cacttgatat gtatgagctt ttaaaaaaaa ataaaaataa
     1021 atgcgaactt gaaattataa aaggcggaga tcattatctg actaagcaca ggaaagaact
     1081 tgttcagctc agaaagaact ggttcaataa atttttaaaa taatgaactt tttatttata
     1141 acagaatgtt aaacaacttg ctaaataaaa ttactaatca gatttcgaat aaatttaaga
     1201 ataaaatcac gaaaggaaaa ttataaatgt acgatccaat aatggtacaa ccgatgcgcg
     1261 aagaagtaac aggaattggt ttcagagaat taatgtcatc cgacgaagta aaaacagaat
     1321 taagcaaaga cggtacaact tttatctttg taaattctgt ctgtggctgt gcagcaggta
     1381 aagcgagacc cggattatcc atagcagtag aatgggcaaa aaataataat gcaatgcctg
     1441 ataatcttgt aaccgttttc gcaggaatgg aaaaagatgc tgtaactgaa gcaagaaatt
     1501 tttttacagg atttcctcct tcatcacctc aaatggcaat gcttaaagac ggtgagatag
     1561 ttcacatgat caacagaatg gacattgaga ataacgatgc aaacgcagtt gctaatttaa
     1621 ttgcaaatgt attcaataag tattgtaaaa aagaagaaac tgagaaagca taaacttcgg
     1681 taaattctca aaaagtattt taagccggag aatttactcc ggtttttttt tggatttaat
     1741 ttttaaaaaa aaacttaatt aatttattta atacaattat ataaatacat taattattta
     1801 taagtttttt aatatattta atcattcaaa aaataaaaaa gatccaaaag attcagtgtt
     1861 taaggcaata caaatttaaa tttgtattaa ctattcccca aataataaac taaatcagaa
     1921 taaaaatgga cagcaatttt tcaagcagag ttcaggatgt tatcagatta agcagagagg
     1981 aagcaattcg tctcgggcat gattacatcg ggacggagca tctgctactg ggaataataa
     2041 gagagggtga aggaatagcc atcaaaatat ttaaaaatct cggagtagac gcttttaaaa
     2101 taaaaaagac aattgaagat accgtaagac aatcaggcgg cactttgact gtcggaaata
     2161 taccgcttac aaaacaagca gagaaagttc ttaagatcac gtatctcgaa gcaaagattt
     2221 tcaaatccga tattatcgga actgagcatt tacttttatc tattttaaga gaagatgaca
     2281 atcttgcagc gcagatactt catcagttta atataaacta tgaagcagta aaatctgaac
     2341 tcatgaacat tctcagcgga aagccgacag gtaaccagcc cggctcaaaa gcttcttcgg
     2401 aaaagaaaca ggatagaagt aaaacacctg tccttgataa tttcggcaga gatcttacca
     2461 aaatggcaat tgaaaataaa ctcgatccta ttgtcggacg ggaaaaagaa atagaaagag
     2521 tagcacaggt tttaagcagg aggaaaaaaa ataatcctgt acttatcggc gaacccggtg
     2581 tcggtaaaac tgcaattgca gaaggacttg cactaagaat aataaataaa aatgtttcac
     2641 gtgttttaca taataaaaga gttgtcactc ttgatcttgc tgcacttgta gccggcacga
     2701 aatacagagg acagtttgaa gaaagaatga aagctgtaat gaatgaactg gagaaagcaa
     2761 aagacgtgat tcttttcatt gacgaacttc atactatagt aggtgcaggt ggcgcaagcg
     2821 gttctctcga cgcatcaaat attttcaagc cggctcttgc ccgaggagat ctgcaatgta
     2881 ttggtgcgac tacacttaat gaatacagac aatacattga aaaagacgga gcacttgaca
     2941 gaagattcca gaagatcatg gtagatccga ctacagtaga tgagactatt cagatactca
     3001 ataatatcaa aagtaaatat gaagagcatc acaatgtgaa ttatacagaa gctgcaataa
     3061 tagaagctgt caaactaagt gacagatata ttacagacag attccttccg gacaaagcaa
     3121 ttgacgtaat ggatgaagcc ggttcgagag tacacctgtc aaacattgtt gtaccaaaag
     3181 aaatcgtaga tctcgaagct gagatcgaag acatcagaca atttaaaaat caggttgtaa
     3241 aaaatcaaaa ttatgaagaa gcagcaaagc ttcgggataa tgagaaaaaa gtacttgctg
     3301 atcttgaaca gttaaaactt gactgggata taaaatcgca aagcatggtc tatgaagttt
     3361 ctgagcaaag cgttgctgat gttgtagcaa tgatgacagg tattcctgta aatcaaattg
     3421 cagaagctga atctgaaaga cttgttaaaa tggaagattc acttagggaa gttgtcatcg
     3481 gtcaggatga agcagtaaag aatatctcac gcgcgataag aagagccaga gccggtctga
     3541 aagatccaaa cagacctatc ggttcatttg ttttccttgg acctacaggt gtcggtaaaa
     3601 ccgaacttgc caagcagctg gctaaattcc ttttcaattc tgaagattca ctgatcagaa
     3661 ttgacatgag tgaatacatg gaaaaattta atgtgtcaag acttgtcgga gctcctcccg
     3721 gatatgtagg ttatgaagag ggtggtcagc ttactgaaaa agtcagaaga aagccttatt
     3781 cagtagtgtt gcttgacgag atcgaaaaag cacatcctga tactttcaat gtattgctgc
     3841 aggttcttga tgatggaata ttaactgacg gacttggaag gaaagttgac ttcaaaaata
     3901 cgattattat tatgacttca aatatcggga caagagattt gaaactcgat aaggtattcg
     3961 gatttggaga aggaacttcc aagacaaatt acgaaaaaat tagatctaca attgaagatt
     4021 ctgtaaagag agtattttca cctgagttcc taaataggat cgacgacatg ataatattta
     4081 aacagttaga gaaatctgaa cttcaaaaga ttgttgagat cggcacacaa aaacttttat
     4141 ctagaattga acttcaggga ttaaaagtag aagtttcaaa atccgctctt gattttttaa
     4201 gtgagaaggg ctttgataaa aactttggag caagaccact gagaagagca attcagaagt
     4261 atcttgaaga tccattgagt gaagagatat taaaaggaaa tcttaaagat tcaggcaaga
     4321 taaaagtaaa acataaaaaa ggttcagaag agttgacatt tacagacgac aaaaaagaaa
     4381 ctgcttcaga aaaagaatct gttgatataa ctgatgaatc aaaagataac gacgataaat
     4441 aaatatgata aacaaagctc cttataatgc aatgataaca ggtgtcggac actacgtacc
     4501 tgaaaggata attggaaacg attactttga agaatatctc gatacttcgg atgaatggat
     4561 aaaatccaga accggtattg ctgaaaggag aagacttgct gcagaacttc ctacttctta
     4621 tatggcaata aaagctatag aatgtctact tgaaaaaaga ggaattggga ttgaagagat
     4681 agatctaatt atagtaggaa cagtaactcc tgacatgatc tttccttcaa ctgcatgtat
     4741 cttgcagaga gaattaaagg cggttaatgc ctggggattt gatcttcttg cagcttgttc
     4801 gggtttctta ttttctcttg caactgctgt tcaatttgta caatccggat ctcataaaaa
     4861 ggtgctggtt gtcggagctg ataaaatgtc agccatttcc aatatggaag acagaaatac
     4921 ctgtattctt tttggggatg gagctggtgt tgtattactc gaaccaactg aagataaaga
     4981 atacggaatt gtcgattcta ttttaaatgt tgacggaaac ggtgaaaaat atcttcatat
     5041 gcttggaggc ggcagtttaa atccaccatc atttgaaact attgaaaaaa aaatgcacta
     5101 cgtttatcag gatggaaaaa ctgttttcaa agaagcagta aaaggtatgg cagatgtttc
     5161 ttatgagatt atgataaaaa ataaccttac atcagaacag gtttcttacc ttgtcccaca
     5221 tcaggctaat atgagaatca ttacagcctg cgctgaacga atgggaattt cgatggataa
     5281 agttatgcta aacattcaca aatacggtaa tactacttcg ggaacaatcc cgatctgcat
     5341 atcagaatac tggcgtgatg gaaaagttaa aaaaggtgac aacttaattc tgtccagttt
     5401 cggagcaggt tatacctggg gatctatact tgtaaaatgg gcaatctaat gaaataagta
     5461 agatttaaaa tattcattta aaacagtccc cggcaatttt tgttaagccg gggatttttt
     5521 tcatacatta tttactgatc cgggttataa taatttttac tatttaattt aaataacatg
     5581 gaacaaatta cgataagatc ttatcctaaa ataaatctgg gactacggat tttgagtaaa
     5641 cgaagtgatg gattccatga cattgagaca atattttacc ctgtaaagat ctatgatgaa
     5701 attggtttca aaattgaaat atccaataca gaatttaatt cagtaattct gaaggcaaat
     5761 aaatcataca ttccattaaa tactgataac ctttgttaca aagcagttct gaattttttt
     5821 aatgaattca gaattaaaga tcctttcaaa attgaaatcg acttaaagaa aaatattcct
     5881 gtcggaggag gtcttggtgg agggagttca aatgccgcta ctgttctcaa atttctaata
     5941 cgctatttta aaattgaaat tgaaagtaac agaaaaagaa taattgacct tgctttatct
     6001 atcggtagtg atgttccttt ttttctaata atgaaaccaa gttatgctga aggcagaggt
     6061 gaaaaaataa agattctaaa tgattttaga gtcaatcatg atatct
//
LOCUS       DAORVQ010000200         6086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8444_length_6086_cov_4.792406, whole genome shotgun sequence.
ACCESSION   DAORVQ010000200 DAORVQ010000000
VERSION     DAORVQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8444_length_6086_cov_4.792406"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLX80_12530"
     CDS             <1..502
                     /locus_tag="PLX80_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21660.1"
                     /translation="IMVTESAKDKITNDKEQKNINPENKNEPVTSQDNLLNTQEEKNS
                     VEQIDNSKNNTFHKYDIKSVKPDSEKNYDQLVTDTQKSNDGIFYEDNVEEELNNEFFI
                     SGLVDFIDSDIESESNESIDIFENSDLEIINSESSEDQNLQNEFRELTPSEQQEILNI
                     LKKTQI"
     gene            509..688
                     /locus_tag="PLX80_12535"
     CDS             509..688
                     /locus_tag="PLX80_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21661.1"
                     /translation="MNNFIKFLLMLFLFVSVNSFAQRDSANFRQMQELEFVFKEKVKS
                     NLGIDDAQTDKLIEL"
     gene            1227..4370
                     /gene="ileS"
                     /locus_tag="PLX80_12540"
     CDS             1227..4370
                     /gene="ileS"
                     /locus_tag="PLX80_12540"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQY21662.1"
                     /translation="MYENLPENIILPELELEILEFWKENDVFKESIKSRPSDRTFTFY
                     EGPPTANGTPGIHHVISRTIKDMICRYKTMRNFRVFRKAGWDTQGLPVEVEVEKKLGI
                     KSKADIEKFGAVEFNNACKESIFTYLKEWEEMTERIGYWIDLNDAYVTYHNEYIESVW
                     WALKKFFENGLIYKGFKILPFCPICESPLSSHEVAQGYQDLKDPSVFVKFKITDGEYK
                     DSDFLVWTTTPWTLPSNAALAVNPDVDYIHIKTENDEKFILAKERAEVINGEYTILKE
                     FNGKELEHTSYARLFNFFDNEKKAFYVTLGDFVTTEDGTGIVHIAPSYGEDDYQIGLK
                     YDLPVFKAVEKNGLFIDKAGAFKNKNFKDADLDIISELKSNGRLYKKEMFVHSYPHCW
                     RHNVPLMYFATDSWFIRTTEYKDKMIKFNNTINWHPKTIGSGRFGKWLEENKDWALSR
                     NRFWGTPLPIWYYKDADGNEHYECIGSIKELKDKAINFDDVYLVESEMDLHKPYIDEI
                     KIKNKDGNEMKRVPQVIDCWFDSGSMPFAQFHYPFENDNLFEKNYPADFIAEGLDQTR
                     GWFYSLHAIGTFLFDEVAYKNLIVNGMILDKNGKKMSKSVGNVVNPFEMIQIYGADVL
                     RWYLIYSSPVGNSKLFNEEDLKELQNKFFDTLINTVRFFILYSNLSDFDHKKDKATNI
                     SDRPIIDKWIISRINSLKKEYLELMEDYDVTRASRILFDFTNDELSNWYIRRNRKRLR
                     NPESESDRLSGYHTLYYVIIELLKMISPVSPFLTDKLFLTLTESDDSIHLSYIDDPSE
                     EYIDSQLEEEMNIAQNIVYLVRSIRVKNNLRIRQPLQQILVPVLNKDDKNKILNVKDI
                     ILEEVNVKELNLLEGNSDIIVKKAKPNFKSIGPKFGKNVKMVQKIINELNNDQISEIE
                     KNQFIETEGIRIQIEDIEVFTEEIEGWILDTKDNITVALDIKLNDELIEEGIVREFIN
                     RVQNFRRNNDFDISEKVEVYFNTSEFISNVLKKNLTYIKSETLSKKIELSNGHEYDFT
                     KTDINGISCDIYIKKLN"
     gene            4412..5236
                     /locus_tag="PLX80_12545"
     CDS             4412..5236
                     /locus_tag="PLX80_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HQY21663.1"
                     /translation="MAKKLAKTGKKASPVKKNKDTIGKKNSNKTSSVKTALKKPVIKK
                     AIVKKAIVKKAVAKKAVINKIAEKNSGNKKPVTKKSVESKKKDIVKTVKKVIAKSKAG
                     LKSLGKSVRVELIKKPVFKNTNEKVVLPVKLKKYKPNKKVKFNSTELKQFRKIILLER
                     KAILESAKANMDSLVDKDSGEYKGDNLTYSSHMAEQGTDEMEREKNYLFVQRDEKYLG
                     YLQDALMRIDQGTYGVCADCKDEPKNLCKTCPLIDRERLELVPITQHCIECKNLRG"
     gene            5244..>6086
                     /locus_tag="PLX80_12550"
     CDS             5244..>6086
                     /locus_tag="PLX80_12550"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQY21664.1"
                     /translation="MKVLFITFFIVIADQVTKILVKGISIPSLGIEIEGMPYQSSKKV
                     FGDLFKLTFIENPGMAFGLQIGGKLFLSLFTIAATLLLIFFLFKNRKENLILRLALAF
                     ILGGAIGNLIDRVFYGEIYSYAPYFYGRVVDFLHIDFPNFTIFGKTIYSWPIFNIADI
                     SVTIGFLMILIGYKKAFPIPPETLTDTPENDQGEIEYKEYFEGESIDSSQNTLFSKQL
                     SSENEELNKESKIEESIDINESEFINEPNIVQSKEDTRKIIIDDLDLSEEKDVEENSI
                     TVPSD"
BASE COUNT         2349 a          807 c         1065 g         1865 t
ORIGIN      
        1 aattatggta acagaatcag ctaaagataa aataacaaat gacaaagaac agaagaacat
       61 taatcctgaa aataaaaatg aaccagttac ttcacaggat aaccttttga atacgcagga
      121 agaaaagaac tctgttgagc aaattgacaa ttcgaaaaat aatacgtttc ataaatatga
      181 tattaaatct gtaaaacctg attctgaaaa aaattacgac caacttgtca ctgatacaca
      241 aaaaagtaat gatggaattt tctatgaaga taatgttgaa gaagaattga acaatgaatt
      301 tttcatttca gggctggtgg attttataga ttcagatata gaatcagaat caaacgaatc
      361 tattgatatt tttgaaaatt cagatcttga gataataaat tccgaatcaa gtgaagatca
      421 gaatctgcag aatgaattcc gtgaacttac tccttctgaa cagcaggaga tattaaatat
      481 tttaaaaaaa actcaaatat aatctaatat gaataacttt ataaaatttt tgctaatgtt
      541 gtttctgttt gtaagtgtaa attcttttgc tcaaagagat tcggctaatt tccggcaaat
      601 gcaggaactt gagtttgtat ttaaagagaa ggtgaaatct aatcttggga ttgatgatgc
      661 acaaactgat aaattgattg aactttaaaa ggaaaataag aaacaattaa gagaactaag
      721 aaaagaagaa aaagagatat ataaagaaat agaagctaat cctgaagctg cagatataga
      781 ttccaaattc agtaagatct ttgagcttga aaaaaagtct cttgaagtta aagaaaatta
      841 ttttaatgct ttaaaaactt tcctctcacc tcagcagatt gcaaagtcgt atattataaa
      901 aagaaaatta tatgaagaat taagaaaaga aattaataaa agagataata aaaacaggaa
      961 gcgaaataaa taaccaaatt ttatttctct taaaaagaga atggattctt agatttttca
     1021 aataaagtta tatttctttt tactgaagtg tattaaataa aatattttca tttcactttc
     1081 aatcttcatt tcaatcttac caaatgaaaa ctattacttt ttactaagtg atttacctac
     1141 ttaaaacatt cttccagtaa gccgtcttat ttcattgaaa ttggctctct atttttttag
     1201 cttactgact tatttaatta aatttgatgt acgaaaatct tcccgaaaat ataattttac
     1261 ctgagcttga gttagaaata cttgaattct ggaaagaaaa tgacgttttc aaggaaagca
     1321 taaaatccag accttcggac agaactttta cattttatga aggtccgccg actgcaaacg
     1381 gaactccggg cattcatcac gtaatttccc gaacgataaa ggacatgatt tgtcgatata
     1441 aaacaatgcg taacttcaga gttttcagaa aagcaggttg ggatacgcaa ggattacctg
     1501 ttgaagtgga agttgaaaag aagctgggta ttaaatcaaa agctgatatt gaaaaattcg
     1561 gagctgtaga atttaataat gcatgcaaag aatcaatatt tacttatcta aaagagtggg
     1621 aagaaatgac cgaaaggatc ggatactgga tcgatcttaa cgatgcttat gtgacatatc
     1681 ataatgaata tattgaatcc gtttggtggg ctttaaaaaa gttttttgaa aatggactta
     1741 tctataaagg attcaagata ctcccgtttt gcccaatttg tgaatcacca ctatcttctc
     1801 atgaagtagc acagggatat caagatctta aagatccttc agtctttgtt aaatttaaaa
     1861 tcacagatgg cgaatataag gattcagatt ttcttgtctg gactacaact ccatggactc
     1921 ttccttcaaa tgctgcatta gctgtaaatc ctgatgttga ttatattcat attaaaactg
     1981 aaaatgatga gaaatttata cttgcaaagg aaagagctga agttattaac ggtgaatata
     2041 caattttaaa agagtttaac ggcaaagaac ttgaacatac atcttacgcc agactattca
     2101 atttttttga taatgagaaa aaagcatttt atgttacgct gggagatttt gttacgacag
     2161 aagatggaac cggtatagtt catattgctc cttcatatgg agaagatgat tatcagattg
     2221 gattgaaata cgatctgcct gtttttaaag cagtcgaaaa aaacggattg tttatagata
     2281 aagccggtgc atttaagaat aagaatttca aagatgcaga tcttgatata atatcagagc
     2341 tgaaatctaa cggtagactt tataaaaagg aaatgttcgt tcattcttat cctcactgct
     2401 ggagacataa tgtaccatta atgtactttg ccacagactc atggtttatc agaactactg
     2461 agtacaaaga taagatgata aagtttaaca acacaataaa ctggcatcca aaaacaattg
     2521 gttcaggcag atttggaaaa tggcttgaag aaaataaaga ctgggcatta tcccgaaaca
     2581 gattttgggg aactccgctt ccgatctggt attataaaga tgctgatgga aatgaacatt
     2641 acgaatgcat aggaagcatc aaagagctca aagataaagc aataaatttt gatgatgtgt
     2701 atttggtgga aagtgaaatg gatcttcaca aaccttatat agatgagata aaaattaaaa
     2761 ataaagatgg taatgaaatg aagagagttc cacaggtaat agactgctgg tttgacagcg
     2821 ggtcaatgcc ttttgctcag tttcattatc catttgagaa tgataatctt tttgagaaaa
     2881 attatcctgc ggattttatt gcggagggtc ttgaccagac acgtggttgg ttttattcat
     2941 tacatgcaat aggtactttt ctttttgacg aggttgcgta taagaatctg atagttaacg
     3001 gaatgattct tgacaagaac ggcaaaaaaa tgagtaagtc tgtcgggaat gttgtgaacc
     3061 catttgaaat gatacagatc tatggtgcag atgtattgag atggtatctg atatattctt
     3121 cgccggtcgg aaactcaaaa ttatttaatg aagaggatct taaagaactt cagaataaat
     3181 tttttgatac tcttataaat actgtcagat tttttatatt gtattcgaat ctttcagatt
     3241 ttgatcacaa aaaagataaa gctacaaata tttctgacag acctataata gataagtgga
     3301 taatttcaag aatcaattct ttaaaaaagg aatatcttga attgatggaa gattatgatg
     3361 ttacaagagc ttcacggatt ttatttgatt ttacaaatga tgaattatca aactggtata
     3421 taagaaggaa cagaaaaagg ttaagaaatc cagaaagtga atctgacaga ttgtccggat
     3481 atcatacatt atactatgtg attattgagc tgctcaaaat gatttctcct gtttctccat
     3541 tcttaacaga taaattattt ttgacattaa ctgaatctga tgattctata catttatcct
     3601 atatagatga tccttctgaa gaatatatag acagtcagct tgaagaagaa atgaatatcg
     3661 ctcagaatat tgtatatctt gtaagatcta taagagtaaa aaataattta cgcattcgtc
     3721 agccgcttca gcaaatatta gttccggtcc tgaataaaga cgacaagaat aaaatattaa
     3781 atgtaaaaga tattatactt gaggaagtaa atgtcaaaga gctgaattta cttgaaggaa
     3841 attctgacat tattgtaaaa aaagctaaac caaattttaa atcaatcggt cctaaatttg
     3901 gaaaaaatgt taagatggtt caaaaaataa tcaacgaact taataatgat cagatctctg
     3961 aaattgaaaa aaatcaattc atagaaaccg aaggtataag aattcagatt gaggatattg
     4021 aagttttcac tgaagaaatt gaaggatgga tcctggatac aaaagataat ataactgtag
     4081 ctttagacat taagctgaat gatgaactga tagaagaagg aatagtaaga gaatttatta
     4141 acagagttca gaatttcaga agaaataatg attttgatat tagtgaaaaa gtagaagtgt
     4201 attttaatac ttcagagttc atttcaaatg tcttgaaaaa gaatctgact tatatcaaat
     4261 ctgaaacact aagcaaaaag atcgagcttt ctaacggtca tgaatatgat tttacaaaaa
     4321 cggatataaa cggaatttcg tgtgacatct atataaaaaa acttaattaa caaaatttaa
     4381 ctatttatct tataatctaa ggagtattaa attggcaaag aagttagcaa aaaccggaaa
     4441 gaaagcatct ccggttaaaa aaaataaaga tacaattgga aaaaagaatt ccaataaaac
     4501 aagttcagtt aaaactgcat tgaaaaaacc tgttatcaag aaggcaattg taaagaaggc
     4561 aattgtaaag aaagcagtag ctaaaaaagc tgtaataaat aagattgctg agaagaatag
     4621 cgggaataaa aagccggtta caaaaaaatc agttgaatca aaaaagaaag atattgtaaa
     4681 gaccgtaaaa aaagttattg caaaatccaa agccggactt aaatctttag gtaagtctgt
     4741 aagagttgaa ttgataaaaa aaccggtttt taaaaacacg aatgagaaag ttgttttacc
     4801 tgtaaaactc aagaaataca aacctaataa gaaagtaaaa tttaactcga ctgaattaaa
     4861 acaattcaga aaaattatat tacttgaaag aaaagctatt ctcgaaagtg ctaaagctaa
     4921 tatggattct ttggtagata aagattccgg tgaatataag ggtgataatc ttacttactc
     4981 ttctcatatg gcagagcaag gtactgatga gatggaaaga gaaaaaaatt atctttttgt
     5041 tcaaagagat gaaaaatatc ttggttattt acaggatgca ttgatgagaa tagatcaagg
     5101 gacatatgga gtatgtgcag actgtaaaga tgaacctaaa aatttatgca agacttgccc
     5161 gctgatcgac agagaaagac ttgagttagt accgatcaca cagcattgta ttgaatgtaa
     5221 aaatttaaga ggatagatct tttttgaaag tattattcat tacttttttt attgtaattg
     5281 ctgatcaggt cacaaagatc ttggttaaag ggatttctat accttcactt gggatagaaa
     5341 tagaaggtat gccttatcaa tcctcaaaaa aagtattcgg tgatttgttt aagcttactt
     5401 ttattgaaaa tccaggaatg gcttttggtt tacagatcgg tggaaaatta ttcctgtcac
     5461 ttttcactat tgcagctact cttcttttaa tcttctttct ttttaaaaac agaaaagaaa
     5521 atcttatctt aagattagca ctggctttta ttcttggagg agcaattggt aatcttattg
     5581 acagagtctt ttacggagag atttattcat atgctccata tttttatgga agagtagtgg
     5641 atttccttca tatagatttc cctaatttca ccatatttgg aaaaacaatt tactcctggc
     5701 caattttcaa catcgctgat atttctgtaa caattggatt tttaatgata cttataggat
     5761 acaaaaaagc atttcctatt ccccccgaaa ctcttaccga tactccggaa aatgatcagg
     5821 gcgaaattga gtacaaagaa tactttgaag gagaatcaat tgatagctca caaaacacat
     5881 tatttagtaa gcaacttagt agtgagaatg aagagttgaa taaagaatcc aaaattgagg
     5941 agtcaataga tataaatgaa agtgaattta ttaatgaacc taatatagtt cagagtaaag
     6001 aagacacaag aaaaatcatt attgatgatt tagacttatc ggaagaaaaa gatgttgaag
     6061 aaaattcaat tacagttcct tcagat
//
LOCUS       DAORVQ010000201         6071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8479_length_6071_cov_4.649934, whole genome shotgun sequence.
ACCESSION   DAORVQ010000201 DAORVQ010000000
VERSION     DAORVQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8479_length_6071_cov_4.649934"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1290)
                     /locus_tag="PLX80_12555"
     CDS             complement(52..1290)
                     /locus_tag="PLX80_12555"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQY21665.1"
                     /translation="MILNTIFLTAVIFFSGVFYKPDVIQKVKIEDQFILGNENLLKNK
                     SEVLSGKKTALITNKSGVMSDGTLFLDELVKNANVRKIFTPEHGLRGDDRDENFTDEN
                     TGLPVISLYGNNKMPVKTDLEDVDVLVYDIQDVGARFYTFINTMFYCMQAANENNIEF
                     VVCDRPMMPNGNYVDGFMLDDGQESFVGMIDIPIAYGMTCGELALYINTEYFGDACKL
                     KVIKMENYSRETDYQSLNLPWVKPSPNIYYPSSALTYLGTCLFEGTNFSEGRGTDKPF
                     EYVGAPYCDGNALAEEMNSLGLNGVTFESIDFVPTTITSPSNPPKYVGENCSGVYIKV
                     TDKNSFEPVKTGIAFLVSLNKLFSGFEIKTNKFLDKLAGTDRLRLAVLNGYTTNEIVS
                     GYQQELQDFKELRKKYLIYK"
     gene            complement(1581..3098)
                     /locus_tag="PLX80_12560"
     CDS             complement(1581..3098)
                     /locus_tag="PLX80_12560"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQY21666.1"
                     /translation="MRIKFEKTSVEKLKTDTYIFLCYQDKKLFKDELDLIEKLLGCKI
                     AKISLEDFEGKDKQTVLVYSDKYRIILSGLGKKEALNLEKVRRSTARGVKKAKTLNIN
                     SVAIEILQDLTLEGVEIEDITRAQTLSSLLALYSFHKYYTKKDNPDLHKISEIIFFSQ
                     TNSLGKYVKEIQEGIRSGSVIGEATNMARDLGNEPSNIMFPAELAKRVQVLAKQNKFS
                     AQILDKSQITKLKMGGVLGVSKGSRHEPKFIIMEYKGGKKNDAPYVIVGKGVTFDSGG
                     ISIKPSAGMAMMKLDMGGACAVVGVFDAISQLDLKLNVIGLIPAVENMPDGDAFKPGD
                     VLIAHNGMTVEIDNTDAEGRLILADALSYASKYKPAAVIDLATLTGAAVVAIGSVASI
                     VMGNDQKLIDKLIEAGHQTYDRVWQLPLWDEYDRMIDSEIADVRNSGVVREAGTILAG
                     MFLKRFTGNYSWAHIDIAGTAMRNKETDYDPKNATGSGVRLVTQYLMNESKKVKK"
     gene            complement(3114..4109)
                     /locus_tag="PLX80_12565"
     CDS             complement(3114..4109)
                     /locus_tag="PLX80_12565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQY21667.1"
                     /translation="MNSNYGKVNELIEGCSNIILTTHVVPDGDAIGSVMAFSEYLKLK
                     GKKPVIINHSETPFNLKFMDTEDIIKVFRDNVDENTKLINNADLIVILDTNDFKRTKS
                     MQEVLEKSAAKKLCIDHHTGLDEKKYTAYISNTEYPSNCSILYDYIKSQGDKYLTKEI
                     SSHLYVGIMTDTGSFRYPRTTSTVFRVCADLIDKGADPVSLYESIFATTTMENLKLMA
                     KFIESFEFYFDNKLVTGTVTQEDFKNLGLGIQHIEGFTSVLMDIQGIRIGVVLVELPD
                     NIKVSFRSKGNIDVSVIAREYSGGGHKNAAGTNIKGVSIEVLKNELLDKLKTYLD"
     assembly_gap    4231..4330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4526..5935)
                     /locus_tag="PLX80_12570"
     CDS             complement(4526..5935)
                     /locus_tag="PLX80_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21668.1"
                     /translation="MREKAFVIWEGTNTKMTFLEFAAYCAPIFWFSPDEPELHLASGK
                     NIQIPTYFPFELPSDSPVVYYQIKDIYPRNNYKGSVFKKNYSDLNNSVLDISKIEAID
                     LDYNHYYRFESGLGGHDHDTEQSSFKIFIEKRKKNGTIYYTFVFLQAIGKAHALSWYD
                     NIYSIDTSAFETKLPFHILVEEGKHASCTDMNGDGYYTPGYDVNQRTNDAWGVRDVIR
                     TGTLFSSSFESYMQKFRRPEHRVFPPLPNDSKLRKYYSDDSTIIYAEYNAVYSLRPMP
                     KPEMAGRDKSLKKDMEGYSQDLWPVVIYKGRELFRWFKAGQVISSVGISYRFDNGLNG
                     FSVTLPLLIVKNVEAPIIGGWIVNRFYMQGKNLDNLGYDILYTPSASRFLDSYVSAGV
                     EFEDINTTDSNNVTTSSLDTYFVMELGVKLRANVRYSPLKFLGFISDFWGIRLGMKNK
                     GFSKISDINYIFEVGAGVW"
BASE COUNT         1877 a         1146 c          843 g         2105 t
ORIGIN      
        1 aacagagaaa aagtatgatc taaaaatatt tatttaaatt taattctgga actatttata
       61 aataagatat ttctttctca gctctttaaa atcctgaagt tcctgctgat aacctgatac
      121 aatttcatta gttgtatatc cgtttaatac tgctaatctc agcctgtcag ttcctgcaag
      181 cttatctaaa aatttattcg tctttatttc aaatcctgaa aataatttat tcaaagaaac
      241 cagaaatgct atacctgtct ttacaggttc aaatgaattt ttatctgtga cttttatata
      301 aacaccgctg caattctcgc cgacatattt cggcggatta gaaggacttg ttattgtagt
      361 aggaacaaaa tctatacttt caaatgtgac tccattcaaa ccaagtgaat tcatttcttc
      421 agccaaagca ttcccgtcac aataaggagc tcctacatat tcaaaaggtt tatcagtacc
      481 tcgtccttca gaaaaattag ttccctcaaa taaacaagta ccaagataag ttaaagctga
      541 agatggataa taaatattag gtgaaggttt tacccacgga agattcagtg attgataatc
      601 cgtttctctt gaataatttt ccatttttat tactttaagt ttacatgcgt caccaaaata
      661 ttctgtattg atatatagtg caagttcacc gcatgtcatt ccataagcaa tcggaatatc
      721 gatcattcct acaaaagatt cctgtccatc atccagcata aatccatcta cataatttcc
      781 gttcggcatc attggtctgt cacaaactac aaattctata ttattttcat tagcagcttg
      841 catacagtaa aacattgtat ttataaaagt ataaaatcta gctccgacat cctgtatatc
      901 ataaactaaa acatcaacat cttctagatc tgtcttaaca ggcattttat tatttccata
      961 aagagaaata acaggcagac ctgtattttc atcagtaaaa ttctcatctc tgtcatcgcc
     1021 tctgagtccg tgttccggtg tgaaaatttt tctaacgttg gcatttttta ccagttcatc
     1081 aagaaacaat gtaccgtctg acattactcc gcttttattt gtaatgagtg cagtcttttt
     1141 accgctaagt acttcagatt tattttttaa taaattttca ttacctaaaa taaattgatc
     1201 ttcaattttt actttttgaa ttacatcggg tttgtaaaat acacctgaga aaaaaattac
     1261 tgcagttaaa aaaattgtat ttaatatcat gtattagatt tttataaata tagtcttccg
     1321 tatttttaaa ttaaataaaa aatgcagtta aatattttta actgcaattc aatgtcaaca
     1381 gctactaagt cttagtagct ttcggaaatc tttgggattg ccccgaagac tctaagactc
     1441 taagcttaaa agcttaaaag cttaaaagtt aaaaagtttt taaacataaa atattaattc
     1501 aaaagagctt tgagcctttg atccttttag gtaaacttaa ttttttttta aaaatttaat
     1561 cgaaagtata actgaaaatt ttacttctta actttcttac tctcattcat taaatactgc
     1621 gtgacaagtc ttacacccga accggtagca tttttaggat cataatcagt ttccttattt
     1681 ctcatagcag tgcctgcaat gtcaatatgt gcccatgaat aattacctgt aaatcttttc
     1741 aaaaacatac ctgcaagaat agttccggct tctctgacta cacctgaatt tcttacatca
     1801 gcaatttccg aatcgatcat tctgtcatat tcatcccaga gaggaagctg ccagactctg
     1861 tcataagtct gatgacctgc ttcgattagt ttatcaataa gcttctgatc atttcccata
     1921 actattgaag caacactgcc gatcgctaca acggctgctc cggtaagtgt agcaagatct
     1981 ataactgcag cgggtttata ttttgaagca taagataatg catctgcgag aattaatctt
     2041 ccctcagcat cagtattatc aatttcgaca gtcattccat tatgggcaat aagtacatca
     2101 cccggtttaa aagcatcacc atccggcatg ttttctacag caggtataag tccgattaca
     2161 ttaagtttca gatcaagctg agaaatagca tcaaacaccc caactactgc acatgcacca
     2221 cccatgtcaa gtttcatcat tgccattcct gctgacggtt ttatagaaat accaccgctg
     2281 tcaaaagtta cacctttacc gactattaca tacggagcat catttttctt tccgccttta
     2341 tattccatga taatgaattt cggttcatgc ctgcttcctt tagaaactcc aagtactccg
     2401 cccattttca gtttagtgat ctgagatttg tcaagtattt gtgcggaaaa tttattttgt
     2461 tttgcaagca cctgaactct tttagcaagc tcagccggaa acattatatt agaaggttca
     2521 ttaccaagat ctctagccat atttgtagct tcaccaatga cagatccgct ccttattcct
     2581 tcctgaattt ccttaacata ttttcctaaa gaatttgtct gagaaaagaa aatgatctca
     2641 cttattttat gaagatcagg attatctttt tttgtataat atttatgaaa tgaatataat
     2701 gctaacaatg agctgagagt ctgagccctg gtaatgtctt cgatctcaac tccttcgaga
     2761 gtaagatcct gtaaaatttc aatcgctact gaattaatat taagagtctt agctttcttg
     2821 actccgcgag ctgtggatct tcttactttt tctaagttaa gagcttcctt tttccctaat
     2881 cccgaaagta tgatcctgta tttatcagaa taaacaagaa cagtttgctt atctttacct
     2941 tcgaaatctt caagactgat ctttgcaatt ttgcaaccta aaagtttttc gatcagatca
     3001 agctcatctt taaacaattt tttatcctga tagcaaagaa aaatatatgt gtcggtcttt
     3061 aatttttcta ctgaagtttt ttcaaattta atacgcataa tagtttttta atattagtct
     3121 aaataagttt ttaatttgtc cagcaattca tttttgagaa cctcaattga aacacctttt
     3181 atatttgtac ccgcggcatt cttatgtccg cctccggagt attcccttgc aattacgctt
     3241 acatcaatat ttccttttga tctgaatgaa actttgatat tatcaggcaa ttctacaagt
     3301 acaactccta ttctaattcc ctgaatgtcc ataagaactg aggtaaatcc ttcgatgtgc
     3361 tgaattccaa gtcccagatt tttgaaatcc tcctgagtta cggtaccggt tactaatttg
     3421 ttgtcaaaat aaaattcaaa actttctatg aactttgcca tgagtttcag attctccatt
     3481 gttgtagttg caaaaatact ttcatataat gaaacaggat ctgctccttt atctataaga
     3541 tctgcgcaaa cccgaaaaac agtagaagtc gttctgggat atctgaaaga cccagtgtct
     3601 gtcataattc caacatacaa atgcgaagaa atttcttttg tcagatattt atctccctgt
     3661 gattttatgt aatcgtacaa tattgaacag ttggaaggat attctgtatt tgaaatgtat
     3721 gcagtatatt ttttttcatc gagtccggtg tgatgatcga tgcaaagttt ttttgcagcg
     3781 gatttttcca aaacctcctg catagactta gttcttttaa aatcattagt atctaagatt
     3841 acaatcaggt ctgcattatt tatgagctta gtattctcat cgacattatc tctaaagact
     3901 ttgatgatat cttcagtgtc catgaacttt aagttgaaag gtgtttctga atgatttatg
     3961 atgacaggtt tctttccttt taatttcaga tattcggaaa atgccataac acttccgatc
     4021 gcatctccgt caggcactac atgtgttgtg agaattatat tgctgcatcc ttcgatcagt
     4081 tcgtttactt taccgtaatt tgaattcaag tattaatatt tattagcaaa cacataaaat
     4141 agaccaaatg aatttttata acaatcgaat attttttact tttatctgaa tcctccttac
     4201 caaacttttt cattcccgag tgctccacaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn ttacttttat ctgaatcctc cttaccaaac tttttcattc ccgagtgctc
     4381 cacaaatagt cattaccccg ttctcatgga gtgaatcctc acgaaaattt tttcacaatt
     4441 attagaatat tttattatcg caagtaaagt ttaaattttc tttgtggtaa ttttatgatc
     4501 atgtaactat tttaaatttt aactattacc aaacccccgc cccgacttca aagatataat
     4561 ttatatcact tatttttgaa aatcctttat ttttcatacc taatcttatt ccccaaaaat
     4621 cagaaataaa tcctaaaaat tttaatggtg aatatcttac gtttgctctc aatttcacac
     4681 caagctccat cacaaaataa gtatcaagac tggatgtagt gacattatta gaatcagtag
     4741 tatttatatc ttcaaattct acaccggcag atacgtatga atctaaaaat cttgaagcag
     4801 acggtgtata taatatatca tatccaagat tatcaagatt ttttccctgc atataaaatc
     4861 tgtttacaat ccatccgcca attataggtg cctctacatt ttttacaatc agtaaaggaa
     4921 gtgtcacaga aaaaccattc agtccgttgt caaatctgta tgaaatacca acagaactta
     4981 ttacctggcc ggctttaaac cacctgaaaa gttctcttcc tttatatata acgaccggcc
     5041 aaagatcctg tgaataccct tccatatctt tttttaatga tttatctctt cctgccattt
     5101 ctggttttgg cattggtctg agagaataaa ctgcattata ttctgcataa attatggtgc
     5161 tgtcatcgga ataatatttt ctgagtttac tgtcatttgg caaaggcggg aatactctgt
     5221 gctcaggtct tctgaatttt tgcatataac tttcaaatga agatgaaaac aaagtaccgg
     5281 tcctgataac atctctcacg ccccatgcat cattagttct ctgatttaca tcgtaacccg
     5341 gagtataata tccgtcacca ttcatatctg tacagcttgc atgttttcct tcttccacta
     5401 aaatatgaaa tggtaatttt gtctcaaaag ctgaagtatc aatagaatat atattatcat
     5461 accagctaag tgcatgtgct tttccaatag cctgaaggaa aacaaaagtg tagtaaatag
     5521 taccattttt ttttcttttt tcaataaata ttttaaaact tgactgctca gtatcatgat
     5581 catgcccgcc cagtccggat tcaaatctgt aataatgatt gtaatccaga tctattgctt
     5641 cgatctttga aatatcgaga acagaattat tcagatcaga ataatttttt ttaaagacac
     5701 ttcctttata attatttctc ggataaatgt ctttgatctg ataataaacc acaggtgagt
     5761 cacttggaag ctcgaaagga aaatatgtag gaatttgaat attttttccg ctggcaagat
     5821 gcagctcagg ctcatcaggt gaaaaccaaa atataggagc acaataagcc gcaaattcaa
     5881 gaaatgtcat tttagtattt gtaccttccc agattacaaa tgccttttcc cgcatttcct
     5941 ttattgatac ttgattcttc gattgggaaa aagattcagc attacaaaat actgttgtca
     6001 ataagatctg tattgcaaat atttttttta aatttttatt caagtatagt tggatattga
     6061 atgacaaata t
//
LOCUS       DAORVQ010000202         6063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8501_length_6063_cov_5.814581, whole genome shotgun sequence.
ACCESSION   DAORVQ010000202 DAORVQ010000000
VERSION     DAORVQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8501_length_6063_cov_5.814581"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PLX80_12575"
     CDS             complement(<1..1325)
                     /locus_tag="PLX80_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21669.1"
                     /translation="MKALLITTFFAASFFSFTNIQSQWINQNVPDNIGVVLSMDFGDL
                     QNGSGCGNIFLSDVYGRAVYTINGGNNWLTSQVPDSSRVLASIQFVTSTTGFCAGAYN
                     TLNNPGNSQLLKNLNVNYSQNGQLLNNSEKTNFSKSGENYKGLFYKTTDAGQSWNTFG
                     NLPPDVFYLNGLSFINSNTGFVTASYHVFGGVDDGILKTTDGGNSWVKLQMPSDTINN
                     LNYIFFLDDNTGYATGFKKYENVPNGIIIKTTNSGTSWTLQRFTNTSSLSNINFPNSN
                     TGFVIGSTFPFVDSSYVFKTINAGNNWELLPIQLNNIFESGIDFVPGSGTGIIYGNIF
                     DTAASLLFTAKTTDYGVTWMNNQFIDESNVIVGSKLLNENNWYLCGQGLNSRGCILHS
                     TNGGVGITQIGNTIPERFHLNQNYPNPFNPKAVINYNLRVAGNIILKVF"
     gene            complement(1349..2638)
                     /locus_tag="PLX80_12580"
     CDS             complement(1349..2638)
                     /locus_tag="PLX80_12580"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21670.1"
                     /translation="MKKNIFILIMLLSGIISEFTYSQTSQWRALTNSPFSSSRFEDIF
                     FLNENTGWIINFDGSTYRTFNGGQNWLFSQNIELNRSVGFFNSQDGIIGTLDIDRPLF
                     RTSNSGANWIQVTNLPNPPPRGVCGISIINQNTAVVCGTYSGNARAYKTTDKGISWST
                     IFSDTSKARSLVDTYFWSPDSGLIAGGYTTTTYSNGVAVVLMTTDGGSTWNTVHKTNR
                     LKEWCWKINFYSRTNGVISIERNSGLSYFLKTSNSGLNWTEVPFREYDQEGIGFINDN
                     TGWIGGWTGPTYQTTNGGTNWSLAGWGENVNRFRFFSDSLGYAVGDRVYKYSRGSVGI
                     NLISAEIPETYKLHQNYPNPFNPKTVINYNLRVGGNIILKVFDVLGNEVSTLVNEKQI
                     EGTYSVDFDGSGLASGIYFYKLEAGEFIETKRMILLK"
     gene            complement(2863..5835)
                     /locus_tag="PLX80_12585"
     CDS             complement(2863..5835)
                     /locus_tag="PLX80_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HQY21671.1"
                     /translation="MKKLLLLLFLILLSVPASQSQTIVATYPFPAYSQYNSFWGITKI
                     GDTLRIATDNNGSIYKVTTTGIIKDSLVTPFNFNHGLEWDGTGYWIAEQFRTAGARLY
                     KINTAGERMDSILLPPLIGGASGGVGDIALDNGGLWFTIYSPDFTVYPYSYAYKINLT
                     TRLITDTIPLLGRQVQGIAVKGDTVFYVNEYFHTTPFVDRERIYAYSLSQRDTLFSFA
                     TPDPDGDGNPRGLHWDGQFLWLIADRIGGNISLFKTLYKYSITGAGNPQITTSNNSLD
                     FGNTIVGMSNDRPFTITNTGTSKLIISAYNITNNLFSINPNNTPDTINPSQVKNYTLK
                     FTPLAFGNVTGQLKIVSNDAVSPEKIINLFGKGIFNGSNIGSSVSSLNYSTRRVNSLS
                     GGYLDITNQGSAPLIINSVTFNSQRFRLDTVGLTFPIVIDTQKTLPMRIWFNPNSAAT
                     FNDSAKFNSNAVNSSVLRIPVTGIGQVSPAVLGNIQWQAEIPDNPNTTSDNLKVMSMK
                     EISDVNSDGINDVMIATDNYWTICYNGNSSVTDDILWKFNSRKSNNVSGSVVYEDGMQ
                     IMNDINGDGVQDVVIGTGGNNELVFALSGRTGNLLWSYGDSLITSDGDINGMNVSKDY
                     NNDGINDVLVAATGEGKGNGRHAAICLNAVNGTAIFYAVQGGHFTHSITPTSVGGAID
                     FSSNGGPYGVNGFSNAGGFTWSTTVSSTVWNLKEMQDLNADGVTDIAAYFGFSGSIRM
                     MSGNNGAQIWSNDFGQSIDGNIRTLGDLNNDGFNDITFSGPQTLYRVDSRTAGVIWNT
                     SLDNNYIHSVAELSDMTGDGIREIAAGTQNSNIYVVRGDSGQIIFTYNFGSATTNTVE
                     QVASIKSIDGNSSSEFLGGSREGIVICFDGGPNGVVGITNINSSLPEKYSLNQNYPNP
                     FNPSTKIKFDIAGKTQNVMLTIYDAIGREVSVLVNQQLTPGSYESEFRADNFASGIYF
                     YKLEAGEFIETKRMILLK"
BASE COUNT         1860 a         1181 c         1022 g         2000 t
ORIGIN      
        1 tcaaatactt tcagtataat atttcccgca actcgtaaat tgtaatttat gactgccttt
       61 ggattgaatg gatttggata attctgattc aaatgaaatc tttccggaat ggtatttcca
      121 atctgagtta ttccgacacc gccgttagtt gaatgtaaaa tacacccacg tgaatttaaa
      181 ccttgaccac ataaatacca gttattttca ttcaaaagtt tactgccgac tataacatta
      241 ctttcatcga taaactgatt attcatccag gtcacgccat agtctgtagt ttttgctgta
      301 aaaagtaatg atgctgcagt atcaaaaatg tttccataaa ttatgcctgt tccggatccg
      361 ggtacaaaat caattccgct ttcgaaaata ttatttaact gtattggaag taattcccag
      421 ttatttccgg cattaatagt tttaaaaaca taagaactat caacgaaagg aaaagtactt
      481 ccgatcacaa atccagtgtt tgaattcgga aaatttatat tactcaatga agatgtgttg
      541 gtaaatctct gaagtgtcca tgaagtaccg gagtttgtag ttttaataat tattccgttc
      601 ggaacatttt catatttttt aaatcctgtt gcatatccgg tattatcatc tagaaagaaa
      661 atataattga gattatttat agtatctgat ggcatttgca atttaaccca actgtttcca
      721 ccatcagttg ttttgagaat accatcatcc acccctccaa agacatgata agaagctgtc
      781 acaaacccgg tatttgaatt tataaaagac aatccattca ggtaaaaaac atcaggaggt
      841 agatttccaa aagtattcca gctttgacct gcatctgtgg ttttataaaa cagacctttg
      901 taattttcac ctgattttga aaaatttgtt ttttctgaat tgtttagcag ctggccgttc
      961 tgtgaatagt tgacatttaa attttttaaa agctgactgt ttccgggatt atttaaagta
     1021 ttatatgctc cggcacaaaa accggtagtt gatgtcacaa actgtattga ggcaagcact
     1081 ctggatgaat ccggaacctg agacgtaagc cagttattac ctccatttat ggtgtaaact
     1141 gctcttccat aaacatcact tagaaatata tttccgcatc cggagccgtt ttgcaaatcc
     1201 ccgaaatcca tacttaaaac cacaccaata ttatcaggaa cattttgatt aatccattga
     1261 gactgtatat ttgtgaaact aaaaaatgaa gctgcaaaaa aagttgtgat taatagagct
     1321 ttcataattg ttttaatttt ttaatttttt attttaaaag gatcatgcgt ttagtttcta
     1381 taaattcacc tgcttctaat ttataaaaat aaataccgct cgccaaacca ctcccatcga
     1441 agtcaacaga ataagttcct tctatctgct tttcatttac aagagtactc acttcatttc
     1501 caagtacatc aaatactttc agtataatat ttcccccaac tcgtaaattg taatttatga
     1561 ctgtctttgg attgaatggg tttggataat tctgatgaag cttatacgtt tcaggaattt
     1621 ctgcagagat taaatttata ccgacagaac ctctcgaata tttataaact ctgtccccca
     1681 ctgcatatcc aagtgaatca ctaaaaaatc tgaatctgtt tacattttct ccccagccgg
     1741 cgagcgacca gtttgtgccg ccgtttgtag tctgataagt cggtccagtc catcctccta
     1801 tccaaccggt gttatcattt ataaatccga ttccttcctg atcatattca cggaaaggaa
     1861 cttcagtcca gtttaatcct gagtttgaag tcttaagaaa atatgacagt cctgaatttc
     1921 tttcaatact gatcacgccg tttgtacgtg agtaaaagtt tattttccag caccactctt
     1981 taagcctgtt tgttttgtga actgtattcc atgtcgatcc tccgtctgta gtcattaaga
     2041 caactgctac tccgtttgaa taagtggtag tagtataacc gcctgcgatc agaccggaat
     2101 caggagacca gaaataagta tcaactagtg accttgcttt agaagtatcg gaaaatattg
     2161 tgctccagga tattcctttg tctgtagttt tataagctct cgcatttcca gaataagtgc
     2221 cgcaaactac agcagtattt tgattaataa ttgaaattcc gcatacaccc cgcggaggag
     2281 gattcggaag atttgtaacc tgaatccagt ttgctccgct gtttgaagtt ctgaataacg
     2341 gtctgtctat atcaagtgtt cctataattc cgtcctgtga gttaaaaaat ccgacgcttc
     2401 tgttgagttc aatattctga gaaaaaagcc agttctgtcc tccgttaaaa gttctgtatg
     2461 tactcccgtc aaaatttatt atccatccgg tattctcatt cagaaaaaaa atatcttcaa
     2521 atctggatga tgagaaaggt gaatttgtta aagctctcca ctgtgaagtc tgtgagtaag
     2581 tgaattctga aattattcca gaaagtaaca tgatcagaat gaagatattt tttttcatga
     2641 tttattctac cgaaagtttt aattattgct gtttattttc atccttccaa agctgcaggt
     2701 ttttccgaaa acaaaattat taagtttgcc tccaggtctt gaaggctcaa agatctaata
     2761 ctttattctt ttaaactttg agcctttgaa gcttagagac gtttcaatcg ttttcagaca
     2821 agctgtatac ttataaagga atgaataatt tttaaatttt ttttatttta aaagaatcat
     2881 acgctttgtt tcaataaatt cacctgcttc taatttataa aaataaatac cgcttgcaaa
     2941 attatctgct ctgaattctg attcatatga gccgggtgtc aactgctgat tcacaagtac
     3001 agatacttcc ctgccgattg catcatatat tgtcagcatt acattttgtg ttttacctgc
     3061 tatgtcaaat ttaattttag tagaaggatt aaatggattc ggataatttt gatttagaga
     3121 atatttctca ggcaatgatg aattaatatt tgtaatacca actactccat tgggtcctcc
     3181 gtcaaagcag atcactatac cttctctgct tccgccgaga aattcagagc tgctgtttcc
     3241 gtctatactt tttattgaag ctacctgctc aacagtattt gtcgtagcag atccaaagtt
     3301 ataggtaaaa ataatttgtc cgctgtcacc tctgaccaca tagatattac tgttttgagt
     3361 accggctgca atctcacgga taccgtcacc tgtcatatct gaaagttcgg cgacgctgtg
     3421 aatataatta ttatcaagtg aagtattcca gataactcct gctgtcctcg aatccactct
     3481 gtaaagtgtc tgcggacctg aaaaagtaat atcattaaaa ccatcattat tcagatcacc
     3541 taatgttctg atgtttccgt caatactctg cccgaaatca tttgaccaga tctgcgctcc
     3601 gttatttcct gacatcattc ttatcgatcc gctgaaaccg aagtatgctg caatgtcagt
     3661 gactccgtca gcattaagat cctgcatttc ttttagattc cataccgtac ttgaaactgt
     3721 tgtagaccag gtaaaaccgc ctgcattaga aaatccgttt actccatatg gtccgccgtt
     3781 actgctgaaa tcgatcgcac cgccgacact tgtcggtgta atgctatgcg taaaatgtcc
     3841 accttgtaca gcataaaaaa ttgctgtgcc gttcactgca ttcagacaaa tggcagcatg
     3901 acggccgtta cctttgcctt cacctgtagc agcgacgagt acatcattta tcccgtcatt
     3961 attataatcc ttggaaacat tcattccgtt aatatcaccg tctgatgtga taagcgaatc
     4021 accataactc cataataaat ttcctgtcct gcctgaaagt gcaaatacaa gttcattgtt
     4081 accgcctgtg ccgatcacca catcctgtac tccatcaccg tttatatcat tcatgatctg
     4141 cattccgtct tcataaacca ctgagccgga tacgttattg cttttacgtg aattgaattt
     4201 ccaaagaata tcatcagtca cagaagaatt tccattgtaa caaatcgtcc agtaattatc
     4261 tgtggcaatc attacatcat taataccgtc actgttcaca tccgatattt ccttcattga
     4321 catcaccttc agattatcag aagttgtatt tggattatca ggaatttccg cctgccattg
     4381 aatatttccc agtactgcag gtgaaacttg accaataccc gtaacaggaa ttcttaacac
     4441 cgaactgttt acagcattgg aattaaattt tgccgaatca ttaaatgtag ctgcactgtt
     4501 tggattgaac catatcctca ttggcaaagt cttttgagta tcaatcacaa tcgggaaagt
     4561 aagtccgact gtatcaagtc tgaatcgttg tgaattaaaa gtaactgaat taatgatcag
     4621 tggagcactt ccctgatttg taatatccag atagccaccg ctcagactat tgaccctgcg
     4681 agtagaataa ttcagagaag ataccgaaga tccaatattg cttccgttaa aaattccttt
     4741 tccaaacagg tttataattt tctccggtga gacagcatca ttgctgacta tcttcagctg
     4801 acctgtcaca ttaccgaaag caagcggagt aaattttaat gtgtaatttt ttacctgtga
     4861 aggattaata gtgtccggtg tgttatttgg atttattgaa aataaattat tagtaatatt
     4921 ataagcagat ataatcagtt tacttgtccc tgtatttgtg attgtaaaag gtctgtcatt
     4981 agacattccg acaatagtat taccaaagtc gagagaatta ttactggtag tgatctgcgg
     5041 attacctgct cctgtaatgg aatatttata aagagtttta aaaagagaaa tatttccgcc
     5101 aatcctgtca gcaatcagcc ataaaaactg accatcccag tggagacctc tcggatttcc
     5161 gtctccgtca ggatcaggag tcgcaaaaga aaataaagta tctctctgag aaagactata
     5221 agcataaatt ctttccctgt ccacaaaagg tgtggtatga aaatattcat ttacataaaa
     5281 tactgtatcg ccttttactg caattccctg aacctgtctg ccgagaagtg gaatagtatc
     5341 agtgatgagt ctcgtggtta aatttatttt gtaagcataa gaataaggat agacagtgaa
     5401 gtctggtgaa taaatagtga accagagtcc gccgttgtca agtgcaatat cacctactcc
     5461 gccggatgca ccgccgatca atggaggcag aagaattgag tccattcttt ctccggctgt
     5521 atttatttta taaagtcttg ctccggctgt tctgaactgt tcagcgatcc agtaacctgt
     5581 accatcccat tcaagtccgt gattaaaatt aaatggagtt accagactgt ctttaataat
     5641 accggttgtt gttactttat agatactgcc gttattatca gttgcaattc gtaaagtatc
     5701 accgatttta gtaatgcccc agaaagaatt gtattgggaa taagcaggaa atggataagt
     5761 agctacaatt gtctgagatt gtgaagcagg tacagatagt aaaattaaaa ataaaagtaa
     5821 taataatttt ttcataattt ataaatttta aaaaaatgtt tgatttaaaa tatacattct
     5881 tctgaaagta gtacacagct gaagagattt tttttattgt cataaaagta tgttaaataa
     5941 tttttttatg aaaagcattt ttttaggaaa aaagtaatag tggcattgcc tcaaattctc
     6001 caagactcaa agtttaggaa taatttcttt ggaaatgcct cgaagtctct aagactcaaa
     6061 gtt
//
LOCUS       DAORVQ010000203         6001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8655_length_6001_cov_6.116717, whole genome shotgun sequence.
ACCESSION   DAORVQ010000203 DAORVQ010000000
VERSION     DAORVQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8655_length_6001_cov_6.116717"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..2101)
                     /locus_tag="PLX80_12590"
     CDS             complement(95..2101)
                     /locus_tag="PLX80_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21672.1"
                     /translation="MKNYTKFFKSGITGFVILVLFALLGTQIFQGSGLNYFMNYDESD
                     PNIDRPTRESLTHYVQGDGTSDIITDAIGFDNFEIGIDNAEQMLVSNPRNPLEMQFGV
                     NGGSGQNAYYTRNGHDWTLSNPSYHPSICCDPWSAWDSLGVLHYGSGVNGQYVYNSTN
                     AGQTYGPAILTVAGNDRNTMAADQTAGPYANYLYAAITPGTFSRSTDGGLTWANTYSS
                     TNQIPGVMIAVGPNGATQGGSVVYVTNTGTSSNVTYVFHHSTNGGATFTVMSSLTVAG
                     FVGTLNTAGRLVINNGRTRPYPMIAMDNSYGPNRGRLYLVYASNEPVGNANKPDIILQ
                     YSDDKAATWSSRIRVNDNPNPELSDQWFPAIWCEKETGRLYMKWYDTRDNPAAYGVNV
                     YATYTDDGGATFAPNQRLTTQTWTYPTPACSPNQNCYRGDYDGMTANEKSSFSVWYDG
                     RQGNYKNIGAYFPDYALKVNPTAVVVTNSNFTTFKAVVPAVKLYTDVTTFSGVVTPTP
                     ASGALTVSFPNGNTLTNYPDSLEVRINAAPNTTPGAYTVTLTSAGSNGTPVHKRTVTL
                     TVGLVGITTSETGIPEKFSLSQNFPNPFNPVTSINFDIAKNGSVKLNVYDISGKLVKE
                     LVNNEMSAGSYSYSLNAADLNSGVYFYRIEANGFVDSKKFMLVK"
     gene            2371..3591
                     /locus_tag="PLX80_12595"
     CDS             2371..3591
                     /locus_tag="PLX80_12595"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21673.1"
                     /translation="MAQINWVRQTSPVTSALFNCVFTDTLNGWAAGEEGIILHTSDGG
                     INWIIQSNPVDYYINDIFFINKNTGWCVSNEFLLNGTTLIRTSNGGNNWIAEPFPDSS
                     VFFRSIFFLDSLNGYLAGFFGTVYKTTDGGNNWNPTFVDTSTYSMYPINKIKFLNAQT
                     GFACGGIIDIAGVMWTSTNSGNNWTAIDYAAEPFYDFSLKDPDRLFAVGGDFEYGAQF
                     VLSSNSGTSWNYNSLGFFGQGQSIDYRTKNEMWMALGFSGAWAFSDDTGHTWVTFPTT
                     DIAAINSVKFVDSLHGWAVGNNGAILKYVYDLVNITLNESEFPNSYSLSQNYPNPFNP
                     ETVIKYSLPVSAFVKLRVYDVLGNEVAELVNEKLNAGSYSVKFSGKNLSSGVYYYNIT
                     ADDFSETKRMILLK"
     gene            complement(3596..5905)
                     /locus_tag="PLX80_12600"
     CDS             complement(3596..5905)
                     /locus_tag="PLX80_12600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY21674.1"
                     /translation="MELAAENKEITIQDKVKNFANHFLSNSFIQITFLLVLKLLFQII
                     ILNSGYRWLSADDFCRTVKSFEWLQRPVIDSGVWLTPHFWINGFVMIFIKDLFLAATI
                     VNVIFSAFNLIFFYKICEICFDKRIAFVSALIFIFFPFQVWLSVSGLPESVFFCFVTA
                     GIYYFLKWRYGGRSNLFLILSALMFAFSNVFRYEGWLFSAVFILLVLIDILKEKKLTK
                     AIIGNFLISLISFFTIVWWLVQNYVDHQNVFFFAEETTKIFKTFNTAGFFQRLVQYPT
                     FIFFIAPITTFFSLKCIFDVLKSPGIPAVKIFLLFNLLELSLLILQGILGTGGTNMIS
                     RYIVINALFFIPYAVSQSFEFKKFLTVIFAVIVIVINVIWSFYYPQPFREDTFEVGKL
                     IREFDQEGYFGDKGKIYFEEVQGYFDVFAVQALSNDPSKFILGDIPTAVISERKSKKD
                     KKVSSEEDLNLLELKKYLEKNNIKLAIVKSDSYSDKLKKLSYKNEHIGDYKLFYLEEK
                     ENDLQNTSLSLLPNNIIQLKENPDVINFNQLLALKNYTIDNTNFGLNPQTVILNWGAV
                     DPNIIDSLDYAEMEYERYYTAIDVRNPESDSVVYSTYYRIFSDRNVVEMIEENNIKNI
                     VVLKPFAILNYSKKYKSSPFEGGVYKIDVRVIDNKYKSDLIVYKSDSLYIPPPDEDED
                     TVIAGKDSRADTIITVKRKPLQKAVREISKSYNLGTVIAMFPDSDYEKIVQLSTTDIY
                     RLLMRNGLQVFFSQRYQGDQFLNWVFNYF"
BASE COUNT         1910 a         1060 c         1016 g         2015 t
ORIGIN      
        1 ggaagaaaaa taattcaaag tttttataca tataaaaaaa cttccgaagt ctcaaagact
       61 ccggaagttt aatatttgta tttagaaaat tactttattt cactaacata aatttctttg
      121 aatcaacaaa tccatttgct tctattctgt agaaataaac tccgctgttt aaatcagcag
      181 cgtttaatga atatgaatat gaacctgcac tcatttcatt attaacaagt tcttttacta
      241 actttccgct gatgtcatat acatttaatt tcactgatcc gtttttggca atgtcaaaat
      301 ttattgaagt gacagggtta aaaggatttg gaaaattttg tgataaagag aatttttcag
      361 gaattcctgt ctcactcgtt gtaattccaa caagtccaac tgtaagagtt actgttcttt
      421 tatgaacagg agtaccatta gagcctgcag atgtcagtgt aacagtatat gcaccgggtg
      481 tagtatttgg agctgcatta attcttactt caagtgaatc aggataattg gttaatgtat
      541 ttccatttgg aaatgaaact gtaagtgctc cacttgcagg ggtaggtgtt acaactccgc
      601 tgaatgtagt tacatctgta taaagcttaa ctgcagggac tacagctttg aaagttgtaa
      661 agtttgagtt ggtaactact actgctgttg gatttacttt taaagcatag tcaggaaaat
      721 atgctccgat atttttataa tttccttgtc ttccgtcata ccagacagag aatgaagatt
      781 tttcattagc tgtcattccg tcatagtcac ctctgtaaca gttttgattt ggagagcatg
      841 ccggtgtcgg atatgtccat gtctgagtgg taagtctttg atttggagca aatgtagcac
      901 ctccgtcatc tgtatatgtt gcatagacat ttactccgta agctgcagga ttatctcttg
      961 tatcatacca tttcatataa agtctgccag tttctttctc acaccaaatt gccggaaacc
     1021 actgatcact aagttcagga tttggattat catttactct aattctgctt gaccatgttg
     1081 cagctttgtc atcagaatac tgaagaatta tatccggttt gtttgcattt ccaactggtt
     1141 cgttagaagc ataaacaaga tagagtctgc ctctgttagg tccgtaactg ttatccattg
     1201 caatcattgg ataaggtctt gtcctgccat tatttataac aagtcttcct gcagtattaa
     1261 gtgtaccaac aaatccggct acagttaaac ttgacataac tgtgaaagta gccccgccat
     1321 tagtcgaatg atgaaataca tatgtaacgt ttgaagatgt tccggtatta gtaacataaa
     1381 caacacttcc gccctgagtt gcaccgttag gaccaactgc tatcataact cccggtattt
     1441 gatttgttga agaataagta ttagcccatg tcagtccgcc gtcagtactt ctggaaaaag
     1501 tacccggtgt gatagcagca tataaataat ttgcataagg tcctgcagtc tgatctgctg
     1561 ccattgtatt tctgtcattt cctgcaacgg taagtatcgc cggaccgtag gtctgtcctg
     1621 cgtttgtgga attataaaca tattgtccgt ttacacctga gccgtaatga agcactccaa
     1681 gactgtccca tgctgaccag ggatcacaac atatggaagg gtgataactt ggattgctca
     1741 aagtccagtc atgtccgttc cttgtataat aagcattttg accacttccg ccgtttactc
     1801 caaactgcat ttcaagtggg tttcttggat tggatacaag catttgttct gcattatcaa
     1861 ttccaatctc gaaattatca aaaccgattg catcagttat aatatctgat gttccgtctc
     1921 cctgtacata atgtgttaat gattctctgg ttggtctgtc aatattcgga tcgctttcat
     1981 cataattcat aaagtaattt aatcctgatc cctgaaagat ctgtgtacct aaaagcgcaa
     2041 acagcaccag tattacaaag cccgtgattc cgcttttgaa aaatttggta tagtttttca
     2101 ttgtattagt tttgtttatt aaataatttt tggttttcat aaaataaagt tatgactttt
     2161 attaatcaac ttaaaaaatg attaaaaaaa atgatgaagg ttgttacatt aagtgactgt
     2221 atttgaaagt ttaaattaaa taatttgata aaagattttt atacctataa taaatgtctc
     2281 caatattgat acctgatttt aattttccag aatatgaagt ttaaattaat cttgttttta
     2341 tttttttcag tagtaattta taaaacctgt atggctcaga ttaactgggt aaggcagaca
     2401 agtcctgtaa catcagcact tttcaattgt gtctttactg atacactgaa cggctgggca
     2461 gccggtgaag aaggaattat tcttcatact tcagacggtg gaattaactg gataattcaa
     2521 tccaatcctg ttgattatta tattaatgat atttttttta taaataaaaa taccggatgg
     2581 tgtgtgtcaa atgaatttct tctgaacgga actactttga tcaggacgtc aaacggtggt
     2641 aataactgga tcgccgaacc attccccgac tcttctgttt ttttcagatc aatatttttt
     2701 ctcgattctt taaatggata tttggcaggg ttttttggta ctgtatataa aactactgac
     2761 ggtggaaata actggaatcc aacttttgta gatacatcta cttattcaat gtatccgatc
     2821 aataaaatta aatttcttaa tgctcagacc ggatttgcct gcggaggaat aattgatata
     2881 gcaggtgtaa tgtggacttc cactaacagc ggaaataact ggacagcaat tgattatgct
     2941 gccgaacctt tttatgattt ttctttgaaa gaccccgaca gacttttcgc agttggaggt
     3001 gattttgaat atggtgcgca gtttgtactt tcatctaact caggaacgag ctggaattac
     3061 aattcacttg gtttttttgg gcagggacag tcaatcgatt acagaactaa aaacgaaatg
     3121 tggatggcac ttggtttttc aggcgcatgg gcattctctg atgatacggg acatacatgg
     3181 gttacttttc cgactacaga tattgccgcc attaattcag taaaattcgt tgactctctt
     3241 catggctggg cagtcgggaa taacggagca attctaaaat atgtgtatga ccttgtcaat
     3301 ataacgctga atgaaagtga atttccaaat agttattcac tttcgcaaaa ttatccaaac
     3361 ccctttaatc cggaaactgt aataaaatac tcattacctg tttcggcatt tgtaaagctg
     3421 agagtgtatg atgttcttgg aaatgaagtt gcggaacttg tgaatgaaaa actaaacgca
     3481 ggtagctatt cagtaaaatt tagcggaaag aacctttcga gcggagttta ttattataat
     3541 ataactgctg atgatttttc tgagactaaa cgaatgatcc tgttgaaatg atttcttaaa
     3601 aataattaaa cacccaattc aaaaactgat ctccctgata tctctgtgaa aagaaaacct
     3661 gtaatccgtt cctcattaat aatctgtaaa tatctgtagt agaaagctgt actatttttt
     3721 cataatctga atccgggaac attgcgatca ccgtccctaa attataactt tttgatattt
     3781 ctctgacagc tttttgtaaa ggttttctct ttactgtaat tatagtatca gcccgtgaat
     3841 cctttccggc tataacagta tcttcatctt cgtccggagg tggaatatat aaactgtcgg
     3901 atttgtaaac aataagatca cttttatatt tattgtcaat tactcttaca tcaattttat
     3961 aaacgcctcc ttcaaaagga gaacttttat acttctttga ataatttaaa attgcaaaag
     4021 gttttaatac aacaatattc ttaatgttgt tttcttcgat catttcaaca acattcctgt
     4081 ccgaaaatat cctgtaatat gtagaataaa cgacagaatc agattcagga tttctcacgt
     4141 ctattgcagt ataatatctt tcatactcca tctcagcata atccagactg tcaataatat
     4201 ttggatccac ggcaccccag tttaaaatta ctgtctgcgg attcagaccg aaatttgtat
     4261 tgtctatagt ataattcttt agtgccagta attgattaaa attgatcaca tcaggatttt
     4321 ctttaagctg tataatatta ttaggcagta atgataatga agtattctgc agatcatttt
     4381 ctttttcctc cagataaaaa agtttataat ctccaatgtg ttcgttttta taactgagct
     4441 ttttaagctt atctgaataa ctgtcgcttt ttacaattgc aagtttgatg ttatttttct
     4501 ctaaatattt ttttagttcg agaagattta gatcctcttc agaagaaact tttttatctt
     4561 tcttactttt tctctcactt ataactgcag ttggaatgtc ccccagaata aattttgacg
     4621 gatcatttga caatgcctga actgcaaaca catcaaagta tccctgtact tcttcgaaat
     4681 aaatttttcc tttatctccg aaatatcctt cctgatcaaa ttcacgaatg agtttgccga
     4741 cttcaaaagt atcctctctg aatggctgtg gataataaaa actccatatc acatttatca
     4801 cgatcacaat tacagcaaag atcacagtta aaaatttttt aaattcaaat gactggctga
     4861 cagcatatgg aataaagaac aaagcattga tcacaatata tctcgagatc atatttgtac
     4921 cgcctgttcc gagaatcccc tgtagtatca gaagtgaaag ttcgagcaga ttgaatagta
     4981 aaaatatttt tacagcagga attcccggac tttttaaaac atcgaatata cattttaaag
     5041 aaaagaatgt tgtgatcggt gcaatgaaga aaatgaaagt aggatattga acaagccttt
     5101 gaaaaaagcc tgcagtatta aatgttttaa agatcttcgt cgtctcttcg gcaaagaaaa
     5161 aaacattttg atgatcgaca taattctgaa caagccacca tacaatagta aaaaaagaga
     5221 tcagtgatat cagaaaattc ccgatgatag cttttgttaa ttttttttct tttagaatat
     5281 ctatcagcac aagtaaaata aatacagcac tgaagagcca tccttcatag cggaatacat
     5341 ttgagaatgc aaacataagt gcagataaga tcagaaataa attacttctt cctccgtacc
     5401 gccatttaag aaaataataa atacctgctg taacaaagca aaagaaaact gattcgggaa
     5461 gtcctgatac gctgagccaa acctgaaaag gaaagaaaat aaatatcagt gcagatacaa
     5521 aagcaattct cttatcaaaa catatttcac atatcttgta aaaaaagatt aggttaaaag
     5581 ctgagaaaat tacattaact atggtcgcag caagaaaaag gtctttgata aatatcatca
     5641 caaatccatt gatccagaaa tgcggagtaa gccagacacc tgaatctatt accggtcttt
     5701 gcagccattc aaaagatttt actgtccggc agaaatcatc ggcagacaac cagcggtatc
     5761 ctgaattaag aattattatc tgaaaaagta atttcagaac taaaagaaat gtgatctgaa
     5821 taaaactgtt tgacagaaaa tgattagcaa aattctttac tttatcctgt attgtaatct
     5881 ctttattttc agcagctaac tccaaatgtt taatcaggct ttttattttg taattttatt
     5941 ctgaatgtcg ttccctttcc aagtgtagaa tcgattaata acaacttccc tttgtgataa
     6001 t
//
LOCUS       DAORVQ010000204         5959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8759_length_5959_cov_5.071308, whole genome shotgun sequence.
ACCESSION   DAORVQ010000204 DAORVQ010000000
VERSION     DAORVQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8759_length_5959_cov_5.071308"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            589..>1087
                     /locus_tag="PLX80_12605"
     CDS             589..>1087
                     /locus_tag="PLX80_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21675.1"
                     /translation="MKLNNLLNSSLFQIIFLSLMVLPFIILCFYNQPTPEDFVFGLET
                     KKLGFLKAQEFFYNNWSGRFFSYAVLSLNAMLFVFIGGYKITTLLLMLVFFSTVYFFV
                     SEFIKEKLNHKEIFLFSLSVIFLYLYKAPSVSEGFYWVTASVIYHLGFILICTFLIFY
                     HKLTKH"
     assembly_gap    1088..1187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1188..2145
                     /locus_tag="PLX80_12610"
     CDS             <1188..2145
                     /locus_tag="PLX80_12610"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HQY21676.1"
                     /translation="RTTHNAQRTTHNAQRTTHNALSIVICSLSVIGISGSSEITGVLF
                     LMILSIILIHNFYKERKISKELVLFGVIFVISFSIAILSSGNNLRASASFPNDHNFYY
                     SVVNSFTLVFKKLLQWIFLSPLLPITIILIPLFNKISDSNTESGSRLFRIKPIYSLLL
                     IPVFLFVQNFIVTWNTGTLHYGRTLNISYLTLLILWFYNVIVVLNFFKLKYKKENFRI
                     PMILNLSSYILIILFLYLSNNIKIAYSEVISGEAKRYNQEMNDRYETIRNSNDENIKI
                     DKIENVPQVFFLYDISEDPNNIYNIWYKEFFGKESIVLKNSE"
     gene            complement(2172..2390)
                     /locus_tag="PLX80_12615"
     CDS             complement(2172..2390)
                     /locus_tag="PLX80_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21677.1"
                     /translation="MHFKFEIENDSTHFRMYILRSQTDTVNILNDYYSAGNYFMNYNT
                     STLGLPNGYYRVYTEMDNLRCFGDVKFE"
     gene            complement(2815..>3006)
                     /locus_tag="PLX80_12620"
     CDS             complement(2815..>3006)
                     /locus_tag="PLX80_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21678.1"
                     /translation="CDLLYPSGKSPNGICDLLYPSGKSPDDICDLLNASGKSQDGICD
                     LNNAWIYFSSAINKFKHAL"
     assembly_gap    3007..3106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3441..4427
                     /gene="paaA"
                     /locus_tag="PLX80_12625"
     CDS             3441..4427
                     /gene="paaA"
                     /locus_tag="PLX80_12625"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HQY21679.1"
                     /translation="MYGGGYIFEESKNGQTETDDVIKLENFEARIAAGEKIEPADWMP
                     AEYRKQLIRMIEQHAHSEIIGALPEGTWITRAPGFRRKLALMAKVQDEVGHAQLLYSA
                     AETLGKTREDMINDLITGKSKYSNVFNYPAVTWADTAIIAWLIDAGAIINQVANSKGS
                     YGPYCRALERICVEESFHLKYGHDNVVFLATGTSKQREMVQEALTRWWPPIMHFFGPS
                     DKISVHTETLMRWKVKMATNDDMRQKFLDMYVPKIWELGLVIPDPLLKKNTDSGIWEY
                     TEPDWQEFKRVINGDGPCNQERLAVRRRAEEKGEWVRKALMRTEEKYTVPLS"
     gene            4483..4899
                     /locus_tag="PLX80_12630"
     CDS             4483..4899
                     /locus_tag="PLX80_12630"
                     /inference="COORDINATES: protein motif:HMM:NF018002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21680.1"
                     /translation="MSDKNIKSINTINSIISIDPRVTREELSIVPDVDPIKDLHHWQT
                     YEVFHQTKKGDRHLHVGSLHAPNPEMAVILAKEQFARRYKCVNLWVVSTGDVISTGYD
                     NEDIFDTTPEKVYREAGGYKVMERINKFKKERKQST"
     gene            4900..5673
                     /gene="paaC"
                     /locus_tag="PLX80_12635"
     CDS             4900..5673
                     /gene="paaC"
                     /locus_tag="PLX80_12635"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HQY21681.1"
                     /translation="MKDLNEIQTTAVKDLLYRIADDQLILGHRNSEWTGLGPILEEDI
                     AFSSMAQDKLGQSQALYQLLNQMGEKDPDTLAFTRSVDEYKCCHLVEYPIGEYDFSLM
                     RHFLFDMSEAIRFNMLASSSFEPLAKISRKFKGEIKYHTMHAITWINQLGKGAGDSPV
                     RIQKALDECFPLALGIFEPSDYEDILIENKIFFGEKELHEKWYDTVVPVLKNANLKIP
                     EIKDPEISYGGRKGKHTEYLKPLIDEMTEVYRIDKDAEW"
BASE COUNT         2058 a          873 c          979 g         1849 t
ORIGIN      
        1 gcagccactc tgaaatttca aaggcattta tagtttaaat taaatgtcaa tgaaagatta
       61 agaaggggtg ctctttattt atttttaaaa attcgtgcaa aacttttaga atgtactatt
      121 tgaaaatcac tttgaaaata aacgaatctg gatttagttg acaacagaga ttatagagat
      181 tcataaaagg ataaaaaaaa atttgaggtt caaaatatac caagcattaa aagaagcaca
      241 agaattcctt ttaaaaattt aataaacttc acatagccaa agcatctgag attatacatt
      301 tatgtataga tcctgaaata tccatttgga tgtccttaat taaagtcata aaataaatgg
      361 tgaatttttg ttgatttttt tatcgttaaa gaaatagcaa ataaaaaata gtgaatcctg
      421 tattttttat ggaaacattt ggaccggtga gatttcaaaa tatcttcacg tttccattga
      481 aataataatg aatcattcat ataatgattt ttcagaaaat tattatttgg gtaggaatga
      541 atattcgaat tcctaatttc tgaacttaaa aaaacttttt tctatattat gaaattaaat
      601 aatctgctaa acagcagttt atttcaaata atctttttga gcttaatggt gctgccattt
      661 ataattctat gtttttataa tcagcctaca cctgaagatt ttgtattcgg attagaaact
      721 aagaagcttg gatttttaaa agcgcaggaa tttttttata ataactggag cggaagattt
      781 ttttcatatg ctgttctgag tttaaatgct atgttatttg tttttatagg tggatataaa
      841 attactacat tacttttaat gcttgtgttt ttttccactg tgtatttctt tgtctctgaa
      901 tttattaaag aaaaattaaa tcataaagaa atatttctct tttctttatc tgtaatattt
      961 ttatatcttt ataaagcacc ttctgtttca gaaggatttt actgggtaac ggcttcagtg
     1021 atctaccatt taggatttat attaatttgc acctttttaa tcttttatca taaattaacc
     1081 aagcacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacg cacaacgcac
     1201 aacgcacaac gcacaacgca caacgcacaa cgcacaacgc acaacgcact ttctattgta
     1261 atatgttcat tatcagttat cggaatatcc ggttcgagtg aaatcacagg agttttattt
     1321 cttatgattt tatcaatcat actgattcac aacttttata aagaaagaaa gatcagcaaa
     1381 gaactggttt tgtttggagt aatatttgtc atatcatttt caattgcaat cttatcatca
     1441 ggaaacaatc tcagagcatc tgcatcattt ccgaatgacc ataattttta ttattcagtt
     1501 gtaaattctt ttaccctagt attcaaaaaa ttattacaat ggatatttct ctcaccactt
     1561 ttaccgatta caattatttt aattccttta ttcaataaaa tttcagatag taatacggaa
     1621 tcaggatcac gtttatttcg gatcaaaccc atatactctt tactgttaat acctgttttt
     1681 ttatttgttc aaaattttat tgttacctgg aatacaggca ctttgcatta tggaaggaca
     1741 ttaaatattt cgtatctgac attattaata ttatggtttt ataatgtgat agttgtttta
     1801 aattttttca aactaaaata caaaaaggaa aatttcagaa taccgatgat cttaaatctt
     1861 tcatcataca tattaattat tttgttttta tatctttcaa ataatattaa gatcgcttat
     1921 tcagaagtaa tctcaggtga agctaaaagg tataatcagg aaatgaatga caggtatgag
     1981 acaataagaa acagtaatga tgaaaatatt aaaattgata agattgaaaa tgtgccgcag
     2041 gtatttttct tatatgacat ttctgaagat ccaaataata tttacaatat ctggtacaaa
     2101 gaatttttcg gaaaagaatc aatagtatta aaaaattccg agtagtaatt ttaatgaatt
     2161 aaaaacttgc atcactcaaa cttcacatcg ccaaaacatc tgagattatc catctctgta
     2221 taaactctat agtaaccatt aggcagtcct aaagttgatg tattataatt cataaaataa
     2281 ttgccggcac tgtaatagtc atttagaata tttacagtat cagtttgact tctgagaata
     2341 tacattctga aatgtgtgga atcattttca atttcaaatt tgaaatgcac agttcgctga
     2401 gaaggattcg gaaggaggat ttaccttatt acatagaaat ggaaaactca cagtaacaca
     2461 ctgcttccag caccagtctg tttcatcccc gccaagaatt acgccattct catatgttat
     2521 taaaatagat gaataatctt cagaggaatt ggtaattgtt tcctttgagc atgaataaaa
     2581 aaacaaaaaa aacgggaata ataaaaatat gaatttgcaa actgacttaa atgacttcat
     2641 tcaaattata ataaaaatta aatttctaat gtctgcaaaa taagtaatat gttttcaatg
     2701 agcaaactat ttacattgtt aaggacacac agctttaaat ccaaaaaatt cattctactt
     2761 atcccgttat ttcatcaact tattttttga taaaattatt aagtcttcag aattttacaa
     2821 cgcatgttta aatttattta tagcactgga aaaataaatc catgcattat ttaaatcaca
     2881 tataccgtcc tgtgatttac cagacgcatt aagtaaatca catatatcgt ccggtgattt
     2941 acctgacgga taaagtaaat cacatatacc gttcggtgat ttacctgacg gataaagtaa
     3001 atcacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacg gataaagtaa
     3121 atcacaaata gcgtccggtg attcacccga cggataaagt aaattacaaa atgcgtccgg
     3181 tgatttacct gatgcattaa gtaaatcaca tataccgttc ggtgatttac ttaatgcgtc
     3241 cgaaaaatta aatgatgcat gtttctattt tcaaagtcag aggaaatatt tataagtaaa
     3301 tatccccccc tttttattgc aacaattctt attgaattgc cacttcagat taaaattcgg
     3361 atttcaaaag taatattttc tcttaattaa aaatgctaaa ttttacaaac aaaaaatttc
     3421 ataaataaaa attatttatt atgtacggtg gcggatatat ttttgaggaa tcaaaaaacg
     3481 gacagactga aacagatgac gttattaaat tagaaaattt tgaagcaaga atagctgcag
     3541 gggaaaaaat tgaacctgcc gactggatgc ctgctgaata cagaaagcag cttatcagaa
     3601 tgatcgaaca gcatgctcat tctgaaataa tcggcgcatt acccgaaggt acatggatca
     3661 cgcgcgcacc cggattcaga aggaaacttg cgctgatggc aaaagttcag gatgaagtag
     3721 gtcatgcaca gcttctttac agtgcagcgg aaactctcgg taagacaagg gaagatatga
     3781 taaatgatct cattacaggt aaatcaaaat attcaaatgt attcaactat cccgctgtca
     3841 cttgggctga tactgccatt attgcatggc tcatagatgc aggtgcgatc attaatcagg
     3901 ttgcaaattc caaaggttca tacggacctt actgcagagc gcttgagaga atctgcgtgg
     3961 aagaatcttt tcaccttaaa tatggtcatg acaatgtagt tttccttgcg actggaactt
     4021 caaagcagag ggaaatggtg caggaagctt tgacaagatg gtggcctccg ataatgcatt
     4081 ttttcggacc ttcagataaa atttccgttc acactgaaac tctgatgcgg tggaaagtaa
     4141 agatggctac gaatgatgac atgcggcaga aatttcttga tatgtatgtt ccaaagatct
     4201 gggaacttgg acttgtaatt cctgatccgt tattaaagaa aaatacagat tcaggaatct
     4261 gggaatatac ggaacctgac tggcaggaat ttaaaagagt cattaacggc gatggacctt
     4321 gtaatcagga aagacttgct gtcagaagaa gagctgaaga aaaaggtgaa tgggtaagaa
     4381 aggctttgat gagaactgaa gaaaaatata ccgttccgct ttcataaaat tctgaataac
     4441 tgcaacggta aaaaataatt attgagctta atctgaaatt acatgtctga taaaaatatc
     4501 aaatccataa atactataaa ttctattatt tccattgatc caagagtcac acgtgaagag
     4561 ctgtctattg tgcctgatgt agatccaata aaagatcttc atcactggca gacttatgaa
     4621 gtgtttcatc agaccaaaaa aggtgaccgg catttacatg taggttcgct gcatgcaccg
     4681 aatcctgaga tggctgtaat acttgcgaag gaacaatttg caagaagata taaatgtgta
     4741 aatctctggg tagtcagtac aggtgatgtg atctcgacgg gttatgataa tgaagatatt
     4801 tttgatacta ctccggaaaa agtttacaga gaagccggcg gatataaagt aatggaaaga
     4861 attaataaat ttaaaaaaga aaggaaacaa tctacataag tgaaagacct taacgaaatt
     4921 caaactactg ctgtaaaaga tcttttatat agaattgctg atgatcagct tatactcggt
     4981 catagaaatt cagaatggac aggtctcggt ccgatactcg aagaagacat tgcattttca
     5041 tcaatggctc aggataaact cggacagtca caggctttgt atcaattgct aaatcaaatg
     5101 ggtgaaaaag atcctgatac acttgcattt acaagaagtg tcgatgaata taaatgctgc
     5161 catctggtcg aatatccaat tggtgaatat gacttcagtc tgatgcgtca ttttcttttt
     5221 gatatgtcag aagctatcag atttaatatg cttgcatctt cttcatttga gccattggca
     5281 aaaatttcca gaaagttcaa aggtgaaatt aaataccata ctatgcatgc aattacatgg
     5341 ataaatcagc ttggtaaagg tgcaggggac agtcctgtca gaattcaaaa agcacttgat
     5401 gaatgttttc cactggcttt gggaattttc gaaccatcag attatgaaga tatacttatt
     5461 gagaataaaa ttttctttgg tgaaaaggaa ttgcatgaga agtggtatga tactgttgta
     5521 cccgttttga aaaatgctaa tttaaaaata ccggaaataa aagatcctga aatttcttat
     5581 ggtggaagga aagggaaaca tacagaatat ctgaaaccac tgatagatga aatgacggaa
     5641 gtttatagga ttgataaaga tgcggaatgg tgagaatcaa tcaggaatta tgaatttgga
     5701 attaggaatt aggaattaag aatttttgat tgaatttttt ttattgatat aggtcaaaat
     5761 ttttaatagc actgttcaga aaaatccaaa agtaattcct cttaaaaaca ggggaacgaa
     5821 ggtggttgtt gcagaaagaa ataacagata ataatttgca gttctatagt ttaaatcagt
     5881 attgtcagtt taaactcaac tatacaaacc tctccagcct cccctttgta caaaggggag
     5941 gagcaataaa agtaagtag
//
LOCUS       DAORVQ010000205         5876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_8946_length_5876_cov_5.851915, whole genome shotgun sequence.
ACCESSION   DAORVQ010000205 DAORVQ010000000
VERSION     DAORVQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8946_length_5876_cov_5.851915"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PLX80_12640"
     CDS             <1..1546
                     /locus_tag="PLX80_12640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21682.1"
                     /translation="AISLPGYRRNVLYEEFSSNSSPACANNNTFLNSFTNTNIQTVNS
                     IVYHTGILGNDTFYTFNPVQNDARKNYYYLVGVPTTFVDGVLITAIPYGDSINLYTPY
                     NTRYNTGTPVSLTVSDQIIGDSVHCIIDLNVLSPVPKGDYRLRINAIERYRTDITAAN
                     GENQFYDIFREMYPDTNGIPILLNPGSQQFTYTYFKNPEWIDSVLYTTVFIQDNLDRE
                     ILNSAKSREIVINKKRPEPVLAVSNKSDLFQIGTEMQNYYPPVTGFDSVFSDLNIELF
                     EAAFPPLGWKVFNRDGFITFKQFSGANGPTIGGVQSVYMNFFNYNIIGQKDSMYSKVY
                     EMLVQDDSVEFDYAYAQYNNFNIDSLIVKISTDGGLTFPTEIFRKGGLQLTTAPQTSS
                     GFVPQNNSEWNRFSYPLQDIVSVNNFSENIPNRFSLDQNFPNPFNPETKINFELPVSG
                     NVSIKIYDITGREIFTLLQEFRAAGKYSVTFNPQGKGLSLSSGVYFYRLNTQGFTDTK
                     RMVLIK"
     gene            1698..2300
                     /locus_tag="PLX80_12645"
     CDS             1698..2300
                     /locus_tag="PLX80_12645"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HQY21683.1"
                     /translation="MKTIIISLVLALLIMTVPKTIFSQISDDDKIGLFYLLEEEKLAY
                     DIYSEMYKKYNHKVFENIMKAEKVHQEHILDLLKVIDINTDQYYKNPGEFTNKSLQNL
                     YNDFILTGSYSLSDAFRAGAMIEENDIKDLRDNFSKTNNANIKALYECLDNASQNHLR
                     AFVRNLKREDINYSPQVLSKEDFDLIISSKNKPGNCFTND"
     gene            complement(2626..2964)
                     /gene="uraH"
                     /locus_tag="PLX80_12650"
     CDS             complement(2626..2964)
                     /gene="uraH"
                     /locus_tag="PLX80_12650"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HQY21684.1"
                     /translation="MSQITTHVLDTSLGRPGKNIKVELFQKNENEWKLTAEGITNDDG
                     RCTDLLEKSNVPEYGIYKIKFYSGDYFNSSDTKTFYPYIEITFQISDDEHYHVPLLLN
                     PFGYSTYRGS"
     gene            complement(2936..3424)
                     /locus_tag="PLX80_12655"
     CDS             complement(2936..3424)
                     /locus_tag="PLX80_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HQY21685.1"
                     /translation="MEKEELKKCCGSEKWVEGMLIEKQVISVDEILTKAEEIWNSLSP
                     EDRLEAFDYHPKIGDMNSLREKYSHSKDLSESEQSGAADATDDILKQLAEMNEDYEKK
                     FGFIFIVFATGKSVGEMLSILKSRISNNAETEMKNASEEQLKITKLRLKNYYESDHNT
                     RT"
     gene            complement(3442..4434)
                     /gene="alc"
                     /locus_tag="PLX80_12660"
     CDS             complement(3442..4434)
                     /gene="alc"
                     /locus_tag="PLX80_12660"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HQY21686.1"
                     /translation="MNDIRSAGFTGLVDLASEKLGGKAIDASDEFFAPKENLLKSSKP
                     IFIPDKYTVNGKWMDGWESRRKRVAGHDWCIIKLGVQGIIRGLDIDTDHFLGNHPPYA
                     SVDACLSNNKIVKENEWKEILAKTPLEPGSQNLLHVSDDNIYNMVRLNIFPDGGVARF
                     RVYGNVKPNWGLKKYKDLIDLAALVNGGRVLVCNDMFFGSKDNLISPGKSKVMGDGWE
                     TKRKRIPGYDWTILKLAASGKIKKIIVDTNLFKGNYPDQCSIEGCFNTEISDHEITDP
                     TVTWKELLKKTSLKGHIENLFENELTDIGSITHVRLNIFPDGGVSRLRLFGTPD"
     gene            complement(4453..5814)
                     /gene="allB"
                     /locus_tag="PLX80_12665"
     CDS             complement(4453..5814)
                     /gene="allB"
                     /locus_tag="PLX80_12665"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HQY21687.1"
                     /translation="MKIGIRSKNVLIGNEVKDACVIIENEKISDLFYPDSINDSEPEN
                     SFEIIDYGDLVIMPSLIDTHVHINEPGREEWEGFETVTKAAAAGGITMLVDMPLNSEP
                     VTTDRKSLDQKINSATGKLYIDVGFYGGAVPENVSELSLLYRCGVLGFKSFMIDSGLE
                     EFRHVTENDLENILKELDPAKDVPLLVHAETNCGFDEKKYLSDHSYSSFLNTRPREWE
                     NKAIEILIRLSKKYDRYIHIVHLSSSDALDMIRQAKSDGIKITVETCPHYLYFNSENI
                     QDNDTRFKCTPPIRENENREKLWEAVEDGTIDLIVSDHSPCTDELKCMEDGNFKTAWG
                     GISSIQLGLSAIWTEAFRRGLSLTEVSKLMSANPAKLIGMEKRKGKIEIGMDADIIVF
                     DPERKFIVDCNKLYHKNKLTPYDGEELQGEVIATYLRGNKIYDNGNISDHPKGRVIFR
                     KTT"
BASE COUNT         1812 a         1171 c          877 g         2016 t
ORIGIN      
        1 tgccatttct ctgcccggat acagaaggaa tgttttgtat gaagaatttt cttcaaactc
       61 cagtcccgcc tgtgcaaaca ataatacttt tctgaattca tttacaaaca caaatattca
      121 gaccgtaaat tcgatcgtct atcataccgg catactaggc aatgatacat tttatacatt
      181 caatccggtt cagaatgacg caagaaaaaa ttattattat ttagttggcg taccgactac
      241 ttttgtcgat ggagttttga taactgcaat tccatacggc gacagtataa atctttatac
      301 tccttataat accagatata ataccggtac accggtatca cttacagttt ctgatcagat
      361 cattggtgac tcagtacatt gcataataga tttaaatgtg ttatcacctg ttccaaaagg
      421 agattacaga ctcaggataa atgcgataga aagataccgg actgatatta ctgctgccaa
      481 cggtgaaaat cagttctatg atattttcag agaaatgtat cccgacacaa atggaattcc
      541 aatactgctg aatccgggaa gtcaacagtt cacatatact tattttaaaa atcctgaatg
      601 gatcgattct gttctttata caacagtatt catacaggat aatttagaca gggaaatttt
      661 aaattcagcc aaaagccgtg aaatagtaat caataaaaaa agacctgaac ctgtgttagc
      721 tgtctcaaac aaaagtgatc tttttcaaat tggtactgaa atgcaaaatt actatccacc
      781 tgtcacagga tttgattctg ttttttctga tttaaatatt gaattattcg aagctgcctt
      841 tcctcctctt ggatggaaag tttttaaccg tgacggattt ataactttca aacaattttc
      901 aggtgccaac ggtccgacca tcggtggagt acagtctgtt tacatgaatt tttttaatta
      961 taatatcatt ggtcagaaag attcgatgta ctcaaaagtt tatgaaatgc ttgtgcagga
     1021 cgactctgtt gagtttgatt atgcttatgc ccagtataat aattttaata tcgatagtct
     1081 tatcgtaaaa atttctacag acggcggtct gacattcccg acagaaattt tccgtaaagg
     1141 gggattacag cttactaccg ccccgcagac cagttcgggt tttgtaccgc agaataattc
     1201 agagtggaac agattttcct atccgcttca ggatattgtc tctgtaaata atttctcgga
     1261 aaatattcca aacagattta gtctcgatca gaattttcca aacccgttca atcccgagac
     1321 aaagataaat ttcgaattac ctgtctcagg aaatgtctca ataaaaattt atgatattac
     1381 aggcagggaa atatttactt tgttacagga attcagagca gcgggaaaat attcagttac
     1441 attcaatccg cagggaaaag gtctcagcct ttccagcggt gtatattttt acagactcaa
     1501 tacgcaggga tttactgaca ctaagagaat ggttctgatc aaataatttt tcagctaatt
     1561 ctcaaaatag tttaactatt cctcctttca tctcatctct ttcttttaga aataatgaca
     1621 cacatcatac catctgaata attttttagt gaaattatta taaataaaaa cagcataaaa
     1681 ccgggaggta atttaaaatg aaaaccataa taatttcact cgtcttagct ttacttatca
     1741 tgacagttcc aaaaactatt ttctcacaga tatccgatga tgataagatc ggattgtttt
     1801 atttacttga agaagaaaaa cttgcctatg atatttacag cgagatgtac aaaaaatata
     1861 atcacaaagt ttttgaaaat ataatgaagg cagagaaagt tcaccaggag cacatactag
     1921 atctgttaaa agtaatagat ataaatacag atcaatatta taaaaatccg ggtgaattta
     1981 cgaataagtc tctgcagaat ctttacaatg attttattct tacagggagc tattctctga
     2041 gtgatgcatt cagagctgga gccatgatag aagagaatga cattaaagac ctgagggata
     2101 atttttcaaa aactaacaat gccaatataa aggcgttata cgaatgtctg gataatgcat
     2161 ctcaaaatca cctgagagca tttgtcagaa atcttaaaag agaggatatc aattattcac
     2221 ctcaagtact ttcaaaagaa gactttgatc tgataatctc ttcaaaaaat aaaccgggga
     2281 attgttttac gaatgactaa aatatatgtc ctgataaaac tttttttaat ttaaagtttg
     2341 atctgatcaa tgttccaaaa cgtctttgaa cagttatatg tgctgcacaa ataaactttt
     2401 tatgaagtag attttttaat ttattcttaa atatcacggt aagtcgaata tccaaatgga
     2461 ttcaacatta attgcgttaa gcaaaaatat actttttgac aaacctgata gtgcttctac
     2521 ggttgacgga aaccgctcgt gtccaaactc ctgtttggat acgaacttgg aattgaaact
     2581 ccgtttcaaa cttacatccg gtaaattaac accgtatgcc acttcttaac tcccccgata
     2641 agttgaatac ccaaacggat tcagcagcaa cggcacatga taatgttcat catcagaaat
     2701 ctgaaatgtg atctcaatat atggatagaa tgtttttgta tctgatgaat taaaataatc
     2761 tcctgaataa aatttaatct tataaatccc atattcaggt acatttgact tttctaaaag
     2821 atccgtacat cttccgtcat catttgttat tccttcagct gttaacttcc attcattttc
     2881 atttttttga aatagttcga cctttatatt tttcccgggt cttcctaaag atgtatcaag
     2941 tacgtgtgtt gtgatctgac tcataataat tttttaatct taattttgtg atctttagct
     3001 gttcctcgga tgcgtttttc atttctgttt ctgcattatt acttattctg ctttttaaaa
     3061 ttgaaagcat ttctcctaca ctcttcccag ttgcaaatac aatgaaaata aatccgaatt
     3121 tcttttcata atcttcattc atctcagcca gctgttttaa tatgtcatct gttgcatcag
     3181 ccgcaccaga ctgttcagat tcagatagat cttttgaatg tgaatacttt tctctgagag
     3241 aattcatgtc acctattttc ggatgataat caaatgcctc aagcctgtct tccggactta
     3301 atgaattcca tatttcttct gcttttgtta aaatttcatc aaccgaaatg acctgttttt
     3361 caatcagcat tccctccacc cacttttccg atccgcagca tttttttaat tcttcttttt
     3421 ccatttttaa atatttaaaa tttaatcagg cgtcccaaac aatctcaatc tgctgactcc
     3481 cccatccgga aaaatattta atctgacatg agtaatgctt cctatgtctg tcagttcatt
     3541 ttcaaataaa ttttctatat gtcccttgag tgaagttttc tttagcagct ctttccaagt
     3601 aactgtagga tcagtaattt catgatccga aatttcagta ttaaaacatc cttctattga
     3661 acattgatca ggataatttc ctttaaataa atttgtatcc actatgatct ttttgatctt
     3721 acccgaagcg gctaatttca ggatcgtcca gtcatatcca ggaattctct ttcgcttcgt
     3781 ttcccagccg tctcccatta ctttactttt tccgggtgat attaaattat cctttgatcc
     3841 gaagaacata tcattgcata caagtactct tccgccgttc accaatgcgg caaggtcgat
     3901 cagatctttg tattttttta atccccagtt tggcttcaca ttcccataca ctctgaatct
     3961 cgccactcca ccgtcaggaa aaatatttaa tctcaccata ttataaatat tatcatccga
     4021 cacatgcaat aaattctgac ttccgggttc aagcggcgtt ttagcaagaa tttccttcca
     4081 ctcattttct tttacaattt tattatttga gagacatgca tctactgaag catacggcgg
     4141 atgatttcca agaaagtgat ctgtgtcaat atccagcccg cggattattc cctgcacacc
     4201 aagctttata atacaccagt catgaccggc aacccgcttt ctccttgact cccagccgtc
     4261 catccatttt ccgttcacag tatatttatc cggtataaaa attggcttcg aagattttaa
     4321 aagattttcc tttggtgcga aaaattcatc actggcatct attgctttac cgccgagctt
     4381 ttctgaagcc aggtcaacca atcctgtgaa gcctgctgat cttatgtcgt tcataaattt
     4441 attaaaattg aattatgtag tttttctaaa aattactctt ccttttggat gatcacttat
     4501 atttccatta tcataaattt tatttcctct taaatatgtt gcaataactt caccctgtaa
     4561 ttcttctccg tcataaggag tcagtttatt tttatgataa agtttattac aatccacaat
     4621 aaactttctt tccggatcaa aaacaataat gtcagcatcc attccaattt ctattttacc
     4681 tttcctcttt tccattccaa ttaacttagc aggattcgcg ctcattaatt ttgatacttc
     4741 tgttaaactt aatccccttc tgaaagcttc cgtccagatt gctgatagtc ccagctgtat
     4801 tgaagaaatt ccgccccagg cagttttgaa gtttccgtct tccatgcatt taagttcatc
     4861 agtacacgga gaatgatcac tcactatcaa gtcaatcgtt ccgtcttcaa cagcttccca
     4921 gagtttttct ctgttttcat tttcacgaat cggaggtgta catttgaatc ttgtatcatt
     4981 gtcctgaata ttttcagaat taaaatacag ataatgagga caggtctcaa ctgttatttt
     5041 tattccgtct gacttcgctt gtcttatcat atctaatgca tctgatgatg aaagatgaac
     5101 aatgtgaata tatctgtcat atttttttga aagtcttata agtatttcaa ttgccttatt
     5161 ttcccattct ctgggacgag tatttaaaaa tgatgagtat gaatgatcac ttaaatattt
     5221 tttctcatca aatccgcagt ttgtctctgc atgtaccaac agcgggacat cttttgctgg
     5281 atctaattct ttgagaatat tttcaagatc attttctgtt acatgtctga attcttccag
     5341 acctgaatct atcataaaag atttaaatcc caagacacca catctgtaaa gtaaactaag
     5401 ttcactcaca ttttcaggga cagcaccgcc ataaaatccg acatcaatat aaagttttcc
     5461 tgttgcagaa ttaattttct gatcaagaga ttttctgtca gttgtcaccg gttcactgtt
     5521 cagcggcata tcgacgagca ttgtaattcc gcctgcagca gcagcttttg taacagtttc
     5581 aaatccttcc cactcttccc gaccgggttc atttatatgc acgtgagtat ctatcagtga
     5641 cggcattatt accaagtctc cataatcaat tatttcaaaa gaattttccg gctcagaatc
     5701 atttatagaa tccggataaa acaaatcaga aattttttca ttctcaataa ttacacacgc
     5761 atccttcacc tcatttccga tgagaacatt tttacttctt atgccaattt tcattcggta
     5821 aaaataattt caaataattt gaataacaaa ccaattgaaa atttcatctg aatgta
//
LOCUS       DAORVQ010000206         5825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_9068_length_5825_cov_4.554939, whole genome shotgun sequence.
ACCESSION   DAORVQ010000206 DAORVQ010000000
VERSION     DAORVQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9068_length_5825_cov_4.554939"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..4222
                     /gene="porU"
                     /locus_tag="PLX80_12670"
     CDS             86..4222
                     /gene="porU"
                     /locus_tag="PLX80_12670"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HQY21688.1"
                     /translation="MTSNSVSRTCNILFSFAVFCFSVLFTEFTYSQRTEIAATDKTTE
                     TSGSEIESDEEIIDYRILNSNESFIELEFIPVYKNEIEFENSVSSLDSKGSPDIKYRA
                     FPLFFPAAGNNIRVEIMDASSSEEINSDIKPVPNYIYNPKSKEYVPVYDKDKKVYEQN
                     SFYPSNPVSAENSGSIRNKYFGYLKIYPIQYNPVTNNIKKFSSVRVRVSYNGSPEYMN
                     KSLSTEEIQFMQGLAVNSDVAVNWTTKEFNAIRDQPVVNSVLASGDFYRMEVRESGMY
                     KLDKNYLQNAGINLDNVDPRTIKIYGSGGGDLPFNNADTVPYDLLENRILVTGENDGQ
                     FNDNDYVVFYGRNPNEWKFDSFYKSFFHKLNKFSKVNYYWITFGGAQGQRMETVNSVN
                     NQGITPLPYFQDKIFEETEVNNLGSTGYLWISQRIGINESFSFNKELKGYVAGSNVNL
                     RFRFGNGSSLINSYWRLEDLNSSYITTQQVNPIAGYSRINLSYISNNILGINYPLSPG
                     KTGINFRASLRSQDGNSQNIAGYYDYLEVLYKRSFTADNNSLRFNSPDTSGIMEYQIS
                     GYSDPDNVKIFDVTNITSPLIINPISFNGGTLKFQDNINLGKPKEYYALNPNNYKTPL
                     SVSARVPNQNIKGEFADGAAFIIISPKEFMAAANRLKDKRQQQGSNYLKTIVVDIDQI
                     YNEFSNGMMDPVAMRNFVKFAYSNWQERPVYILFLGDGSYDYKNNYNLYNGNLKNWIP
                     PIEKNSQYSDDVESFCSDDYILEITENNNEPSSTNIVDFASGRINANTLLEANNYIDK
                     IFDYENPANFDKWRNEAMYVADDGWTTENTQGQEGSLHTSQCEDVAENNSPKNLIKDK
                     VYIVSYPTEITPQGRRKPGANVDIIKYWNNGKLIINYTGHGSVDLWAHERIFERQISI
                     PQLTNAKKLPFITIASCDLARWDDPFILSAAEQLVLIKDKGSIGISAAVRPVYASSNA
                     IFNNALYRNLFAPDSLNLRLRLGKAMFNVKQQYYADNDLKFALLADPTLRLGVPQHRT
                     KIDSINSTPSTEKFQMKALQKVKVYGSVIKPDSTFWSDYNGVLDMDVLDVDKNIRILD
                     FQSYFNFKLQGGKIFSGKVNIVNGNWVMEYVVPKDISYNPGNGKIISYFKNSNSDGIG
                     FFNNFIIDGQDPNAPVDTTGPQVNIFLDSRNFRTGDLVNQNPKLIIDFFDENGINLTG
                     TIGHKLEAVLNDDENNKIDLTQYYISSSGYQNGSVNYDFSDLAEGKYKLEVNAWDTYN
                     NLKSEYIEFNVAANGELALQNVYNYPNPMTDITNFMFQHNSESPVTAQIRIYTVSGRL
                     IKELNKSNITDKFVSIEWDGKDSDGDNIANGTYLYKILIQSNDGTLSKNSIGKIAMLK
                     "
     gene            4251..5393
                     /gene="porV"
                     /locus_tag="PLX80_12675"
     CDS             4251..5393
                     /gene="porV"
                     /locus_tag="PLX80_12675"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HQY21689.1"
                     /translation="MTLKKLCILLAIFGIIAFDSADVKAQNSVSTGVPFLLIGPNSRF
                     GGMGETGTAIADDATAMHWNPSGLAFQNEGSEINFTHSPWLAGLNIGDLFYDYIAGRT
                     YIKSINGTVGASLTYLNIGEVIVTDEFGNAQPDRNYKAYEMALALSYATMLGKSWGGG
                     IAGRFIYSRLSPNDVTVGNEQGTGTGISAALDLSALWRPVSGASFLKNKFSTGMALSN
                     FGPAISYVDQDQADPLPTLFRFGFAYEVVKTEFNTLTLTADFSKLMVNRELAVVDDSG
                     VVLVPGSVDPVYQAIFTSWKGGVSGIAKSFQSSIGAEFWYGSPRLIALRAGYFYEDPS
                     YGNRNFFTLGAGIRYSMYGFDFSYLSTIEENHPLENTLRFGLQVKF"
     gene            5465..>5825
                     /locus_tag="PLX80_12680"
     CDS             5465..>5825
                     /locus_tag="PLX80_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21690.1"
                     /translation="MFRKIIFVFSALIILTTSEIFSQNKPELKLSHPLKFGAQNKYSS
                     QPKEYIQGADSFKIVAILVQFQEDSDPKSSGNGLFDLSNKYLNPSTGKDTVIDSPPYD
                     SAYFADHLLFIKNYLSKS"
BASE COUNT         2055 a          896 c         1047 g         1827 t
ORIGIN      
        1 acgaaatggt ttttctattt aaaaaaagaa atttttaaga atgttttttg ttaacgcaca
       61 agcaattaat aaaaaataaa aaaatatgac ctccaattct gtatcgagaa cctgtaatat
      121 tttattttca tttgcagtat tttgtttttc tgttttattc acggaattca cgtactccca
      181 aagaactgaa attgctgcaa ctgataaaac tactgagacc tccggatctg aaatcgaatc
      241 agacgaagaa attattgatt acagaatttt aaactcaaac gaatctttca tcgagttaga
      301 atttataccc gtttataaaa atgaaattga atttgaaaat tcggtaagtt cattagattc
      361 gaaaggaagt cccgatataa aatacagagc attcccttta ttctttcctg cagcaggaaa
      421 taatataaga gtcgaaataa tggatgcatc ttcttcagaa gaaattaaca gcgatataaa
      481 accagttccg aattatattt acaatcctaa gagcaaagaa tatgtaccgg tatatgataa
      541 ggacaaaaaa gtttatgaac aaaattcttt ctatccaagc aatccggtta gcgcagaaaa
      601 ttcaggatcg ataagaaaca aatattttgg ttatctaaaa atttatccta tccagtataa
      661 tcctgttaca aataatataa aaaagttttc ctcagttcgc gtcagagttt cttataatgg
      721 aagtcctgaa tacatgaata aatctttaag cacagaagag attcaattta tgcaaggttt
      781 agctgtcaat tcagatgttg ctgtaaactg gacaacaaag gaattcaatg caataagaga
      841 ccagccggtt gttaacagtg tacttgcttc aggtgatttt tacagaatgg aagtaagaga
      901 atccggaatg tataaacttg ataaaaatta tctgcaaaac gccggtataa atctggataa
      961 tgtagatcca aggacaatca aaatttatgg atccggcgga ggagatcttc cttttaataa
     1021 tgctgatact gtaccatatg atctgcttga aaatagaata ttagtaactg gtgaaaatga
     1081 cggtcaattc aatgacaatg attatgtggt tttttatggt cgcaacccaa atgaatggaa
     1141 gtttgattct ttttataaat ctttttttca taaattaaat aaattttcaa aagtaaatta
     1201 ttactggatt acatttggcg gagctcaggg tcaaagaatg gaaaccgtga actcagttaa
     1261 taatcaggga attactcccc ttccctattt tcaggataaa atttttgaag agacggaggt
     1321 caacaacctc ggttcaaccg gttatttatg gataagtcag agaattggaa taaatgaaag
     1381 cttttcattt aacaaagagc ttaaaggtta tgttgcaggg tcaaatgtaa acttgagatt
     1441 cagatttgga aatggttctt ctttaattaa ttcttattgg agacttgaag atttaaattc
     1501 cagctatatt actactcagc aagttaaccc catagccggt tattcacgta ttaatttatc
     1561 ttatattagt aataacatat taggaataaa ttacccgcta agtccaggca agacgggtat
     1621 taatttcaga gcgtcactga gatcacagga tggtaattct caaaatattg ccggatatta
     1681 tgattattta gaagtacttt ataagagaag ttttactgct gataacaact ctctgagatt
     1741 caattctcct gatacaagtg gtataatgga atatcagatt tccggatatt ctgatccgga
     1801 taatgtaaag atctttgatg ttacaaatat tacttcacca ttaataatta acccaatttc
     1861 atttaacggc gggacattaa aatttcagga taatataaat ttaggcaagc caaaagaata
     1921 ttatgcatta aatccaaata attataagac acctctttct gtttcagcca gagttccaaa
     1981 tcagaatatt aaaggcgaat ttgcagacgg cgcggctttt ataatcatta gccctaagga
     2041 attcatggct gcagcaaacc ggcttaaaga caagaggcag cagcaaggca gcaattatct
     2101 taaaacaatt gtagtggata ttgaccagat atataatgaa ttttcaaacg gcatgatgga
     2161 tccggttgca atgcgtaatt ttgtgaaatt tgcatatagt aactggcagg aaagaccggt
     2221 atatatatta tttttaggtg atggcagtta tgactataaa aataattata atctttataa
     2281 tgggaattta aaaaactgga tacctccaat tgaaaaaaat tctcagtatt ctgacgatgt
     2341 agaaagtttt tgcagtgatg attacatatt agagatcaca gaaaataata atgaaccatc
     2401 cagcactaat atagtagatt ttgcgtccgg tagaattaat gcaaatactt tacttgaagc
     2461 gaataattat atagacaaaa tatttgatta tgaaaatcct gcaaactttg ataagtggag
     2521 aaatgaagca atgtatgtgg cagatgatgg atggacaact gaaaatactc aaggtcagga
     2581 aggcagtctt cataccagtc aatgtgaaga tgtagcagaa aacaattctc ccaaaaattt
     2641 aataaaagac aaagtatata ttgtaagtta tccaactgaa attactcctc agggcagaag
     2701 aaaacccggc gcaaatgttg atattataaa atactggaat aatggtaaac ttataataaa
     2761 ttacaccggt cacggaagtg ttgacctatg ggcacatgaa agaatttttg aaagacagat
     2821 ctcaattcct cagcttacaa acgcgaaaaa acttccattt attacaattg cttcctgtga
     2881 tcttgctcga tgggatgacc cttttatact aagtgctgca gaacaattag tactcatcaa
     2941 agacaaagga tctatcggaa tttctgctgc agtgagacct gtttatgcaa gttcaaatgc
     3001 aattttcaat aatgctttat atagaaattt atttgcacca gattcattaa atttaagact
     3061 acgtttaggc aaggcgatgt ttaatgtaaa gcaacaatat tatgctgata atgatctgaa
     3121 gtttgctctg ttagcagatc cgactttaag acttggcgtt ccacagcacc gcactaaaat
     3181 tgacagtata aacagtacac catctactga aaaattccag atgaaagctc ttcaaaaagt
     3241 aaaagtttat ggttcagtaa ttaaacccga ttcaactttc tggtcagatt acaatggagt
     3301 actcgatatg gatgtacttg atgtagataa aaatatcaga atactggact ttcagagtta
     3361 ctttaatttt aaacttcagg gaggaaagat attctctggt aaagtaaata ttgtgaatgg
     3421 aaattgggta atggagtatg tagtaccaaa agatatttca tataatccgg gtaatggaaa
     3481 gataatttca tattttaaaa acagtaattc tgatggtata ggatttttta ataatttcat
     3541 aattgatgga caggatccaa atgcccctgt tgatactact ggacctcagg ttaatatttt
     3601 tttagacagc agaaatttca gaaccggaga tcttgtaaat cagaatccaa aactcattat
     3661 agattttttt gatgaaaatg gaataaacct aaccggaacg attggacata aattagaagc
     3721 agtattgaat gatgatgaga ataataaaat tgatctcact caatattata tcagttcatc
     3781 aggttatcaa aacggttctg tgaattacga cttttcagat cttgcagaag ggaaatataa
     3841 acttgaagta aatgcatggg atacatacaa taatctgaag tctgaatata ttgaatttaa
     3901 tgttgcagca aatggagaac ttgcacttca gaatgtttat aattatccga acccgatgac
     3961 agatatcaca aactttatgt ttcagcataa ctcagaaagt ccggtaacag ctcagatcag
     4021 aatatataca gtaagtggca gattaataaa agaattgaat aaaagcaata ttactgataa
     4081 atttgtgtcc attgaatggg acggaaaaga ttctgacggc gataacattg ccaacggtac
     4141 ttacctttac aaaattttaa tacaatcaaa tgatggaaca ctttcaaaaa attccatcgg
     4201 aaaaattgca atgcttaaat aatcatatgg aggttaaaaa cttaatgtta atgacattaa
     4261 aaaaattatg cattttatta gcgatatttg gaattattgc ttttgacagt gcagatgtaa
     4321 aagctcaaaa ctctgtttcg acaggtgtac cgtttttact tatcggacct aattccagat
     4381 ttggtggaat gggtgaaaca ggaacagcaa ttgcagacga tgcaacagca atgcactgga
     4441 atccgtctgg tcttgcgttt caaaatgaag ggtctgaaat aaactttact cactcacctt
     4501 ggctcgcagg cttaaatatt ggtgatttgt tttatgatta tattgccgga agaacatata
     4561 tcaaaagcat aaacggaact gtgggagcaa gtttgactta tctgaatatc ggtgaagtta
     4621 ttgttactga cgaatttgga aatgctcagc ctgacagaaa ctacaaggct tatgaaatgg
     4681 cactggcact atcttatgct acaatgctgg gtaaatcttg gggcggtgga atagccggac
     4741 gttttatata cagtagatta tctccgaatg atgtaactgt aggaaatgaa caaggtacag
     4801 gaactggaat atctgcagct ctagatcttt ctgcattatg gagacctgtc agcggtgcaa
     4861 gttttctgaa aaataaattt tctacaggaa tggctctttc taattttggt cctgcaattt
     4921 catacgttga ccaggatcag gctgatccac ttcctacact tttcagattt ggatttgctt
     4981 acgaagtagt aaaaactgaa tttaatactc taactcttac tgctgatttt tcaaaattaa
     5041 tggttaacag agaattagca gttgtagatg attcaggtgt agtattggtt cccggatctg
     5101 ttgacccggt atatcaggct atatttacat cctggaaagg tggtgtatca ggaattgcta
     5161 agtcttttca gtcatctatt ggagcggaat tctggtacgg aagtccaaga cttattgcac
     5221 taagagctgg ttacttttat gaagatcctt cttacggtaa cagaaatttc tttacattag
     5281 gtgcaggtat aagatatagt atgtatggat ttgatttcag ttatctaagt acaattgaag
     5341 aaaatcatcc tctggaaaat acactccggt ttggattaca ggtcaagttc taaatttaaa
     5401 tctttttaaa aaaatataac catagttcat agcaagagct atggttatta ttataattta
     5461 ataaatgttt cgaaaaataa tttttgtttt ctctgccctt attattctga caacatctga
     5521 aatcttttct cagaataaac ctgaactaaa actatctcat cctttaaagt ttggagctca
     5581 aaataaatat tcatcacagc caaaggaata tattcagggt gctgactcat ttaaaatagt
     5641 tgctattctt gttcagtttc aggaagactc tgatccgaaa agttcaggaa acggattgtt
     5701 tgatctatca aataaatatc tgaatccgtc tacaggaaaa gatactgtta tagattcacc
     5761 gccatatgac agtgcctatt ttgctgatca tttattattc ataaaaaact atttatcaaa
     5821 atctt
//
LOCUS       DAORVQ010000207         5587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_9761_length_5587_cov_5.914317, whole genome shotgun sequence.
ACCESSION   DAORVQ010000207 DAORVQ010000000
VERSION     DAORVQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9761_length_5587_cov_5.914317"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLX80_12685"
     CDS             complement(<1..199)
                     /locus_tag="PLX80_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014363763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HQY21691.1"
                     /translation="MKYEIDFPEDTEITGLCYDSRNVKPGNIFFAVKGLISDGNNFIK
                     EAIEKGAVLVFTDDEKFSESQL"
     gene            complement(245..2443)
                     /locus_tag="PLX80_12690"
     CDS             complement(245..2443)
                     /locus_tag="PLX80_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQY21692.1"
                     /translation="MQFKNYKSPNKDLTQRRKINLLFLLLSGMFIAILIKLVNVQIIE
                     TEKYKIAARKQYENKIVLSPYRGLVFDRNMNVLVSNSYEYSIAADPNMIEDAEKAADY
                     LALKFGKNRDEYFSKLNSTNTSYIFLERRIDESKTSGLDTLNLSGIIVLKEPKRVYNY
                     GSLASQVLGFTNIDNKGQMGLELSMEKELAGKEGFVIMQRDGKGNNRPSMEFPRKEPE
                     NGNNLVLTIDINIQRIAEEELAEGVKKFNADGGKVIVMSVKTGEILGMSSYPTFDPNN
                     ITTADTSGMKNAVLSDIYEPGSTFKIVAAAAALEEKIENKNSIIATESINEVKGMKIT
                     DVHGSSSMTFQEVLEQSSNVGFSKIAMKIGSEKFYKYARDFGFGISSGIELPGENKGL
                     LKKPIEFSGFSLPYMSIGYEVMVNALQITNAYSAIANNGVMMKSYIIKKETSSSGGTV
                     FENKPTVLRQIISENTAITLSQLLTGVVERGTGVDAKLEGMQVAGKTGTSQRLVNGEY
                     TNKSHNASFAGYFPAENPKVIITVILNNPKSGEYYGGKVAAPIFKNIASKILSLSNLP
                     SFTAPENNISNVSNLNNVTSESVQGENKFIPVKQVLIPNFVNLKLEDAKDILFEKNMR
                     YEIIENDEVMIRDGNIKIIESQYPEANQLLDLSEEIIVKLVVKDAKITDDRLVAVPDV
                     KELSLRKAINLLLSEGLDMEIIGSGKVVGQSPETGTKLLPRSRVILYCKD"
     gene            complement(2459..2767)
                     /locus_tag="PLX80_12695"
     CDS             complement(2459..2767)
                     /locus_tag="PLX80_12695"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQY21693.1"
                     /translation="MKKPNKKISIFYFLICLIITSVILVIYINNIIQVNQIMVRNNEL
                     KEELRKAVQKNEQLLTETEKLTSFDRINSLASEKLELSYKENSTVEAKNITIKNSELK
                     "
     gene            complement(2829..3785)
                     /gene="rsmH"
                     /locus_tag="PLX80_12700"
     CDS             complement(2829..3785)
                     /gene="rsmH"
                     /locus_tag="PLX80_12700"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQY21694.1"
                     /translation="MIIFAMMIEENFHIPVLSNKAVEFLINEKISDQILVDGTLGGGG
                     YTKLICEQLDFSGKVISIDKDENALSHAGEVLSEFKDRIIFVNGNFADIKDIIPETKL
                     THITGIVLDLGLSSYQLNAEDGFSFMQDTPLDMRAYKKDSLTASEILNSYRKEELAEL
                     FKKFGDIGNADRLSEAIYKKRKVKKFETTFDMVETVNSEYSLGQKNQIDFLAKIFQSL
                     RIEVNNELGNLEKVLTDSTELLISGGRIVIVSYHSLEDRIVKNFFRDNTRTYEKTDNP
                     YFDKEIEPVLKILTKKPEVPTREEIKSNPRSRSAKLRAAEKI"
     gene            complement(3831..4304)
                     /gene="mraZ"
                     /locus_tag="PLX80_12705"
     CDS             complement(3831..4304)
                     /gene="mraZ"
                     /locus_tag="PLX80_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQY21695.1"
                     /translation="MFIGHSVCNLDAKSRLILPAKFRKNINPEADNKLVLTKGMDGCV
                     AVYPYDEWERENDRLASLNKFNIEDRYFLRDFMFNATECEIDSQNRILIPSSIIVIGG
                     LKKEILLTGAMKFMEIWDPERKNIYDNSIQTPIEEIAERVTEKNISQQNKNSGDS"
     gene            complement(4889..>5587)
                     /locus_tag="PLX80_12710"
     CDS             complement(4889..>5587)
                     /locus_tag="PLX80_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQY21696.1"
                     /translation="PVDILKIKGVAEVQEYLVNEIQEVYRIQGVKINDKHIEVIVRQM
                     LQKVKVSDTGDTNFLEGDVVNKILFAEKNEDLKGMVVVTDPNGYDKVTENQVYSKKEA
                     KEMNAALKKKDKPLFKVRDAVPATAEPVLLGITQTSLTTESFISAASFQETTKVLTDA
                     AIRGKVDHLLGLKENVIIGQLIPAGTGLRKYKEIIVTEKSEYQEVVKAEDSSDEEKPK
                     KKAKAKKKKTEEVD"
BASE COUNT         1620 a         1058 c          777 g         2132 t
ORIGIN      
        1 gcaattgtga ttcagaaaat ttttcatcat cagtaaaaac cagtacagca cctttttcta
       61 tcgcttcttt tataaaatta tttccatcgc tgatcagacc tttgactgca aaaaaaatat
      121 tacccggttt tacatttctt gaatcatagc atagtccggt tatttccgta tcctcaggaa
      181 aatcaatttc atatttcaaa gaactactcc gaattttttc ggatatttct gaaagtttca
      241 ttttctaatc tttgcagtat aaaatcactc ttgatcttgg cagcaatttc gttcctgttt
      301 ccggagactg accgaccact tttccgctgc cgattatttc catatccaga ccttcggaaa
      361 gcaaaagatt tattgctttt ctcagactta gttcttttac atcaggcact gccacaagtc
      421 tgtcatctgt tatctttgca tcttttacta caagctttac aataatttct tcactcagat
      481 ccagcaattg atttgcttca ggatactgtg attctattat tttaatattt ccatccctga
      541 tcattacttc atcgttctct ataatttcat aacgcatatt tttttcaaac aaaatatctt
      601 ttgcatcttc taatttcaga tttacaaagt ttggaatcag cacctgtttt accggaatga
      661 atttattttc accctgaact gattcgctgg ttacattatt taaatttgaa acattggaaa
      721 tattattttc aggagctgta aaactcggta agtttgaaag acttaatatt tttgatgcaa
      781 tgtttttaaa tatcggagct gcaactttcc caccataata ttctcctgat ttcggattat
      841 tcagaatgac tgtaataatt accttaggat tttcagcagg gaaatatccg gcaaaagaag
      901 cattgtgaga tttgtttgta tattctccgt tcactaatct ctgagaagtt cctgtctttc
      961 ccgcaacctg cattccttct aactttgcat caactcccgt tccccgctct accactccgg
     1021 taagaagctg acttaatgta attgctgtat tctctgaaat tatctgtctc aagactgtcg
     1081 gcttattttc aaatactgta cctccgcttg aagaagtttc ctttttaata atatatgatt
     1141 tcatcattac accattatta gcaattgctg aatatgcatt agtgatctga agtgcgttga
     1201 ccattacttc atatccgatg gacatatatg gcagtgaaaa tcctgagaac tcaattggct
     1261 ttttgagcaa tcctttattt tcacccggta actcaatacc cgaactaatt ccaaatccaa
     1321 aatctcttgc atatttataa aacttttctg agccgatctt catcgcaatt ttactaaacc
     1381 cgacattgct tgactgctcc agtacttcct gaaatgtcat acttgaagat ccgtgtacat
     1441 ctgtgatctt cattcctttt acttcattaa tactttctgt tgcaataata gaatttttat
     1501 tttcaatttt ttcttcaagt gccgctgctg cagctacaat tttaaaagtg gatccgggct
     1561 cataaatatc tgaaagtact gcattcttca taccggatgt atcagcagta gtaatattgt
     1621 tcggatcgaa agtcggatat gaactcattc caagtatctc gcctgtcttc actgacatta
     1681 caattacttt tccgccgtct gcattaaact ttttaacacc ttcagcaagc tcttcttccg
     1741 ctatcctctg aatattaata tcaattgtca gcacaagatt atttccgttt tcaggttctt
     1801 ttcgcgggaa ttccatcgat ggtctgttgt tgcctttacc gtctcgctgc attatcacaa
     1861 aaccttcttt gcctgcaagt tctttttcca tagaaagctc caggcccatc tgacctttgt
     1921 tgtcgatgtt tgtaaatcca agtacctgag atgcaagact tccgtaattg taaactctct
     1981 ttggttcttt aagaacaata attccactca gatttaaagt gtcaagaccg gatgttttac
     2041 tttcgtcaat ccttctttct aaaaagatat aagaggtatt cgtggaattt aattttgaaa
     2101 aatattcatc cctgtttttc ccgaatttta acgcaagata atctgcagct ttttccgcat
     2161 cctctatcat gtttggatca gctgcgatag agtattcata agagtttgag acaaggacat
     2221 tcatatttct gtcaaagacc agtcccctgt aaggagataa aactatttta ttttcatatt
     2281 gcttccttgc agctatttta tatttttcag tttcaatgat ctgaacgttc acaagtttta
     2341 tcaaaatagc tataaacatc ccggagagta aaagaaaaag cagattgatt ttccttctct
     2401 gggtcagatc tttgttggga gatttatagt ttttaaactg caaaaagtta tagtaaaatt
     2461 atttcaattc tgaattttta attgttatgt tctttgcttc aactgtagaa ttttctttat
     2521 aggataattc taatttctcg gatgctaaag aatttattct gtcaaatgat gtaagctttt
     2581 ctgtttcagt caaaagctgt tcgtttttct gtacagcttt tctgagctct tctttcaatt
     2641 cattatttct taccattatc tgatttacct gtatgatgtt atttatatag ataacaagta
     2701 ttacagaagt gatgataaga cagattagaa aataaaagat tgagattttt ttgtttggct
     2761 ttttcatttt gtttaaatgg ttaatattgt ttacaaatat tatttggtaa aattcttttt
     2821 aattgaaatt aaattttttc tgctgctcta agtttagcac ttctcgatct tggatttgat
     2881 tttatttctt ctcgtgtcgg aacttcaggt ttcttagtta aaattttcag aaccggttct
     2941 atctctttgt caaaatacgg attatcggtt ttttcataag tccgtgtatt atctctgaaa
     3001 aaattcttta ctattctgtc ttcaagtgaa tgataagaga ctataacaat tcttccgccg
     3061 gatatcaaaa gttctgtaga atctgtaagt actttttcaa gattcccaag ttcattattt
     3121 acttcaatcc tcagtgattg aaatatctta gccaggaaat ctatctgatt cttttgtccg
     3181 agactgtatt cactgtttac tgtttccacc atatcaaaag tagtttcaaa ttttttaact
     3241 tttctttttt tgtaaatcgc ttctgataat ctatcagcat tccctatatc gccaaacttt
     3301 ttaaagagtt ctgccaattc ttcttttctg taagaattta aaatttcaga agctgtcagt
     3361 gaatcttttt tgtaagctct catatcaaga ggagtatcct gcataaaact gaacccgtcc
     3421 tctgcattta actgataaga tgaaagcccg agatcaagta caattcctgt tatatgagtc
     3481 agtttcgttt ccggaattat atcttttatg tcagcgaaat ttccattaac aaaaattatt
     3541 ctgtctttaa attcagacag gacttcacct gcatgactca atgcattttc atctttatcg
     3601 atcgaaatta ctttcccact gaaatcaagt tgttcacaaa ttaattttgt ataaccaccg
     3661 ccgcctaatg tcccgtcgac taagatttga tcagatattt tttcattgat cagaaattcg
     3721 acagctttat ttgaaagaac aggtatgtga aagttttctt caatcatcat tgcaaatatt
     3781 atcaaacatt aacttcacat atattttttt aaatatcttt taaaaactct ttaactgtct
     3841 cctgaatttt tattttgctg tgatatattt ttttcagtta ctctctctgc aatttcttcg
     3901 atcggagtct gaatggaatt atcataaata ttttttcttt caggatccca gatctccata
     3961 aatttcattg caccggttaa taaaatttct tttttcagac cgccaattac tattattgaa
     4021 gatggaatta aaattctgtt ttgagaatca atttcacatt cggttgcatt aaacataaaa
     4081 tcccttagaa aatatctgtc ttcaatatta aatttattta atgaagctaa ccggtcgttt
     4141 tctctttccc actcatcata aggataaact gcaacgcaac cgtccattcc ttttgtcagg
     4201 acaagtttat tatctgcttc aggatttata ttttttctga attttgcagg taatatcaat
     4261 cttgacttgg catctaaatt acagaccgaa tgtccgataa acattgttga gttttattta
     4321 tttaatagct ttgttatttt gagataagaa aaaataataa tttcttaata tttttctaaa
     4381 aaaagtgtga aattacgatg tttttagcat cctccacttt cctccacttt accccactga
     4441 tatacaaaaa tacgcaaaat aatattaatg tcaaatcgat atttaaagga aagttaatgt
     4501 ttttttaaaa atttaaagga tttctatatt ggagaattgt gacgaggaat tttacaagat
     4561 cgatcgaaaa aattatgttc tgatttcaaa ttctgtttgc tttagaatgg aatttattca
     4621 gatatctatt tggaaaatgt tctgattttt taaaacacaa tatctgtaaa aaaatagttt
     4681 aaaattaatt tatcaatgaa catattcttt ttccaaagag tccgaatagc acatgacttt
     4741 gcggagacgg agatcaccat tatttattca atccattcaa aattaattta acattgaaca
     4801 ttaaaaattg aacattaaaa attgaacatt aaaaaaagcc gtctctcttc agaaacggct
     4861 tttaatattt taccaaaaag taattatgtt aatctacttc ttcagtcttt ttctttttag
     4921 ctttagcttt tttctttggc ttttcttcat cagatgaatc ttcagctttt acaacttcct
     4981 gatattcact tttttcagtt actatgatct ccttatattt tctgagacct gtacctgccg
     5041 gaataagctg accgatgatc acattctctt ttaatccaag taagtgatcg acctttcctc
     5101 tgatagctgc atccgtcaga acttttgtag tctcctggaa tgaagctgca gaaataaagc
     5161 tttcagtcgt aagtgaagtc tgagttatac caagcaacac cggttcggct gtagcaggaa
     5221 cagcatctct tactttgaat aacggtttat cttttttctt aagagccgca ttcatttctt
     5281 tagcttcctt cttgctgtaa acctgatttt ctgttacttt atcatatccg tttggatcgg
     5341 ttactacaac cattcctttc agatcttcat tcttctctgc aaataaaatt ttatttacaa
     5401 catcaccttc aaggaaatta gtatctcccg tgtctgagac ttttactttc tgaagcatct
     5461 gtctaactat aacttcaatg tgtttgtcat tgatctttac accctgaatt ctgtaaactt
     5521 cctgaatttc atttacaaga tattcctgaa cttcagcaac acctttgatc ttcagaatat
     5581 caacagg
//
LOCUS       DAORVQ010000208         5573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_9801_length_5573_cov_5.090069, whole genome shotgun sequence.
ACCESSION   DAORVQ010000208 DAORVQ010000000
VERSION     DAORVQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9801_length_5573_cov_5.090069"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLX80_12715"
     CDS             <1..889
                     /locus_tag="PLX80_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21697.1"
                     /translation="PRKEVFYNHNVISSDNGTILLASDMFKEGREFIKLLKINTNLQV
                     TDDKEILGKERTGDVRIYSNGNYGAFLVWKDKSFNSNLIMGGEFDKNLNTSYPENGDI
                     LSCEQSKGTEERILPDIRIKNSMTVYNNNFFINWTNTDANRLFISNMNLTDESYLCIN
                     TTEIQNSIDDGEYTSFTSQSDKLVIVYKYFNNIFAEIRELNEATKYERTIGYRLGNFP
                     NPFNPTTSINFFIPKDGFVSLKVYDITGKEVRTILNGFKPSGEHKVTFTANELSSGAY
                     FYRLDANGIILTRRMTILK"
     gene            1269..3878
                     /locus_tag="PLX80_12720"
     CDS             1269..3878
                     /locus_tag="PLX80_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQY21698.1"
                     /translation="MNISNILVQKTSKIIFSLLIFLNSVSISAQIKVFERPVETDISD
                     VGLFFNSDTREKISLNGPWEASFNEGKSYSTFNVPLAYDFSGKAFFKKKITIDPEILK
                     TKSFIFVAEGINYETEIKINNNFVSSHTGGYSPIIVPLSDGVVTAQTEITVSINSILN
                     FKNTTPLSDQINYSRVFGGINKDIYLIAVPKVFVFNNFIKYNIDNLLSVKFTNYIEIK
                     STLLKDFDDSVSANEFFIKSKLTRKSNQEEIFMSEPVQFEMVDNNTIKIENFISISNP
                     VLWSPETPELYSLNIQITDKTGNIIDESISETGFNDLTKSNDQIFQSGKQISLKGINY
                     YEDMPGIASALSYREVEKDLQNIKSLGFNAVRVPGRTSHPYIVNVCNRIGLFLLQEIP
                     LNEISTHYFNEERFVNSVLDYLTEIIKRDRNEPSIFAWGLGNDFDVSSSTSLEYVKSA
                     VTIIDSLNSRFKYYTSRPFNSDICSELVDFVGINFYESDYNSIKKDIAELTNNSSPVS
                     NRKNRAYFLSYYGINIQNGNSNGFSDIRSQESQVKYFKELYPSVSKSFFGNFMSSYAD
                     WNAENPLNYPMDINPHLKTNGIVTFNREQKLSTNFIKRLLNNEDLSRIQEGNFVTEFP
                     YVFTIIGIMLMMTFAFFINKDRKFRSGLVRCLYKPTYFFTLVKDQMIISRGYNLLLAL
                     SISIGIGIFLSSVLYFFNDNNSFDMLLSKIFTNNESKIYFSEIVNNKFYLISALSFLN
                     ILLTFLISFFLYFISFYTKGKSYFKIIYTVCIWSTLPMLIFLIIGTVLYKLAESNPVY
                     IKLSLWLFGILMIFSLNRIIIGAKTLFDIRTGRVYLYGIAIIIIFFTLIYSYFYFFTG
                     AVETYDLITNLTS"
     gene            3898..>5573
                     /locus_tag="PLX80_12725"
     CDS             3898..>5573
                     /locus_tag="PLX80_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY21699.1"
                     /translation="MYLSKIEIFGFKSFADKTTIEFDSGVSAIVGPNGSGKSNIVDAL
                     RWVLGEQGDKVLRSGKREDVVFNGTKFRKPLSVAEVAVTIQNNKNILPTEYSEVQIAR
                     RFYRSGETEYFLNGTKVRLKDIKELFIDTGIGPDAYSVIELKMVETILSDVKHERRKL
                     FEEAAGVVSYKHNRDLTFNRLDAVHESLHRVNDIIREKQRNVNALERQAKRNEEAKIV
                     SEELKSLELKTGKFEYESLVREITDIHEKEDDNIKLKESLAIEVRSNDENLDVFRAEL
                     SALDETIIKLTEEHDSIRESANIIERENLVTEEKISNFTVNNDRLKKDNENLQTNILR
                     NKEKEEELTQNVKVLANTIDISEGSLADKKSKVETSIKVINEKKNEMLELAKNAKGSV
                     KIVNDKKELYQKNKITFENNLIQLEKLSSLNSELLIQSEDIGKSINKLESDINRLNDE
                     LKASDKILKDKENQKAALGKILVAKDKELQELDIEFHKLQNKADHLRELSENIDDYAE
                     GIKFLLQEEKGKNISTILDAIEVEDKFKIAIETALGEISNYLIVDDSKDL"
BASE COUNT         2029 a          840 c          922 g         1782 t
ORIGIN      
        1 cccgagaaaa gaagtttttt ataatcataa tgtcatttct tctgacaatg gtactattct
       61 tttggcttct gatatgttca aagaaggacg ggaatttata aaactattaa agattaatac
      121 aaatcttcag gtaactgatg ataaagaaat tttaggaaaa gaaagaaccg gtgatgtaag
      181 aatttattcg aatgggaatt atggggcatt tttagtatgg aaggataagt cttttaattc
      241 aaacctgata atgggtggtg aatttgataa gaatttaaat acttcatatc ctgaaaatgg
      301 agatatactt tcctgtgaac aatctaaagg aaccgaagaa agaatactgc cggatattag
      361 aattaaaaat tcaatgacag tttataataa taatttcttt ataaactgga caaatacaga
      421 tgctaacaga ttatttattt ccaatatgaa tttaactgac gaatcctatc tatgtatcaa
      481 cactactgaa attcaaaaca gcatagatga cggagaatat acttcattta catcacagtc
      541 ggataaactt gtgattgtat acaaatactt caacaacatt ttcgcagaaa ttagggaatt
      601 aaacgaagca acaaaatatg aaagaacaat cggctaccga ttgggtaatt tccctaatcc
      661 cttcaatcca actacgagca ttaacttctt tattcccaaa gatggattcg tcagtttaaa
      721 agtatatgat attacaggaa aggaagtgag gaccatatta aacggtttca aaccaagcgg
      781 tgaacataaa gttacgttca ctgcgaatga actatcttca ggtgcatatt tttacagact
      841 cgatgcaaat ggaattatat taacgagaag gatgactata ctaaagtaaa tttgcttgct
      901 tgctttccgc taaatccgga tgctccttaa caggagctcc gggttttttt ttatattcca
      961 tcttacatac ataaattcga ttctttttta agatccaatt gtattcattt tctttagatt
     1021 ttccaaaatt acaattacaa attttacctc taagattcaa aggctcaaag aatttaactt
     1081 ttatgttttt aaactttgag tcttggagtc ttggagtctt ggagtcttag aggtaaatcc
     1141 aaagttttaa gaccagcaat attaaaaaaa atgagttaat aatgtttcat attaaaaaga
     1201 tactttataa ttcaatactt taaattcctt atgctatttg ttatatttgc atatctttta
     1261 aatcattgat gaatatttct aatattttag tacaaaaaac cagtaagatt attttttcac
     1321 ttttgatctt tctgaattca gtttctattt ctgcacagat aaaagttttt gaaagaccgg
     1381 ttgagacaga tatttctgat gtcggattat tttttaacag tgatacccgt gagaaaataa
     1441 gtctcaatgg tccgtgggaa gcttcattta atgaaggcaa atcttattca actttcaatg
     1501 tccctcttgc atatgatttt tcaggcaaag cttttttcaa aaaaaagatc acaatcgatc
     1561 cggagatcct gaaaacaaaa agttttattt ttgttgctga agggatcaat tatgaaactg
     1621 agatcaaaat taataataat ttcgtttcca gtcatacagg gggttattct ccaataatag
     1681 taccactttc ggatggtgta gtcacagctc agactgagat cacagtaagt attaacagca
     1741 tcctgaattt caaaaataca actcctcttt cagaccagat taattattca agagtttttg
     1801 gcggaatcaa taaggatata tatttaattg ctgttccaaa agtttttgta tttaataatt
     1861 tcattaaata taatatagat aatctcttat ctgtaaaatt cacaaattat atagagataa
     1921 agtccacttt attaaaggac tttgatgact cagtttctgc aaatgaattt tttataaaat
     1981 caaagctgac cagaaaatct aatcaggaag aaatatttat gtctgagccg gtccagtttg
     2041 aaatggtgga caacaataca attaaaattg agaattttat ttccatcagc aacccggtat
     2101 tatggtcgcc tgaaactcct gaactttatt ccctaaatat tcagataaca gataagacag
     2161 gtaacattat agacgaaagt atctcagaga ccggctttaa tgaccttaca aaaagtaatg
     2221 accagatatt tcaaagcggg aaacagatca gtcttaaagg tataaattat tacgaagaca
     2281 tgccggggat tgcatctgct ctgtcttata gagaagttga aaaggatctg caaaatataa
     2341 aatcacttgg atttaatgct gtcagagtac ctggcaggac ttcacatcct tatatagtaa
     2401 atgtatgcaa ccgaattggg ttatttctgc tgcaggagat tccgctaaat gaaatttcga
     2461 cacattattt taatgaagaa agatttgtaa attcagtttt ggattatctt actgagataa
     2521 ttaaaaggga cagaaatgaa ccgagcattt ttgcctgggg acttggcaat gactttgatg
     2581 tttcttcaag tacttcttta gaatatgtaa aatcagcagt tactataatt gactctttga
     2641 acagcagatt taaatattac acctccagac cttttaattc tgatatttgt tccgagcttg
     2701 tagattttgt tggcataaat ttttatgaaa gtgattataa ctctataaaa aaagatattg
     2761 ctgaattaac aaataattcc agcccggttt ccaacagaaa aaacagagcg tattttcttt
     2821 catactatgg catcaatatt cagaatggca acagtaacgg ctttagtgat atcagatctc
     2881 aggagtcgca ggtaaagtat tttaaagaat tatatccttc cgtatcaaag tcattttttg
     2941 gaaattttat gtcatcctat gctgactgga atgcagaaaa tcctttgaat tatcccatgg
     3001 atattaatcc tcatttaaag acaaatggta ttgttacttt taacagagag caaaaacttt
     3061 cgaccaattt tattaaaaga cttttaaata atgaagatct gtcgagaatt caggaaggga
     3121 attttgtaac agaatttcct tatgtattta cgataattgg tattatgtta atgatgacgt
     3181 ttgccttttt tataaataaa gacagaaaat tcagatccgg tctagtcaga tgtctttata
     3241 agcctactta cttttttact ttagtaaaag atcagatgat tatttcaaga ggttacaact
     3301 tattactggc actgagtatt tcaattggta tcggaatttt tctctcttca gttctttact
     3361 ttttcaatga taacaattcc tttgatatgc ttctgtcaaa aattttcaca aataatgaat
     3421 ctaaaatata tttcagcgaa atcgtaaata ataaatttta tttaatctca gcactgtcat
     3481 ttctaaatat attattaact ttcttaatat catttttttt atattttatt tcattttata
     3541 caaaaggaaa atcttatttt aagatcattt atactgtttg tatctggtcc acattgccaa
     3601 tgttgatatt tttaataatc ggcacagtac tatataagct ggctgaatct aaccctgtat
     3661 acattaaact gtcattatgg ctttttggaa tattaatgat cttcagtctg aacagaatca
     3721 taattggagc taaaaccctt tttgacatcc ggaccggaag agtttaccta tatggaattg
     3781 caattattat aatattcttt acacttattt acagttactt ttatttcttt acaggtgctg
     3841 tagagactta tgatctgatt acaaacttaa catcttaata ataattattt aaaattattg
     3901 tatttatcca agatagaaat atttggtttt aaatcgtttg cagacaaaac tacgatagag
     3961 tttgacagtg gtgtatctgc tatagtcggt ccaaacggtt caggtaaatc aaatattgta
     4021 gatgcattaa gatgggttct gggcgagcag ggagataaag tacttcgaag cggtaaacga
     4081 gaagacgttg tatttaatgg tacaaaattc agaaagccat tgagtgtagc agaagttgca
     4141 gttacaattc aaaataataa aaacatccta ccgacagaat attctgaagt tcagatagca
     4201 agaagatttt acagaagcgg tgaaaccgaa tacttcctca acggcaccaa agtccgtctt
     4261 aaggacataa aggaattatt cattgacaca ggaatcggtc ccgacgctta ctccgtcatc
     4321 gagctaaaaa tggttgaaac cattttatca gacgtaaaac acgaaagaag aaaactcttc
     4381 gaagaagccg ccggcgtcgt ttcctacaaa cataacagag atctcacatt caacagactc
     4441 gatgcagttc atgagtcact tcacagagtg aacgacatta tccgggaaaa acaacgtaat
     4501 gtaaatgcgc tcgaacgtca ggcaaaacga aatgaagaag ctaagatcgt atccgaagaa
     4561 ttgaaatctc tagaacttaa aacaggtaag tttgaatatg aatcactggt cagagagata
     4621 acagatattc atgaaaagga agatgataat ataaagctca aagaatcact tgcaattgaa
     4681 gtgagatcaa atgatgaaaa tcttgatgta ttcagagcag aactctcagc tctcgatgag
     4741 acaattataa aattaactga ggaacacgac tctatccgcg aatcagcaaa cattattgaa
     4801 agagaaaatc tggttactga agaaaagatc agcaatttta ctgttaataa cgaccgactg
     4861 aaaaaagata atgaaaatct tcagacaaat attttaagga ataaagaaaa ggaagaggag
     4921 ttaactcaaa atgtaaaagt ccttgcaaat actattgata tttctgaagg ctctcttgct
     4981 gataaaaaat caaaagttga aacaagcatt aaagtaatta atgaaaagaa aaatgaaatg
     5041 cttgaacttg caaaaaatgc aaaaggatct gtaaaaatag taaatgataa aaaagaactt
     5101 tatcagaaaa ataaaattac atttgaaaat aacctgatac agctggaaaa actttcttct
     5161 ttaaactctg aattactaat tcagtctgaa gatataggta aatcaattaa taaacttgaa
     5221 tcagatataa acagattaaa tgacgaactc aaagcttcag ataaaatctt aaaggataag
     5281 gaaaatcaaa aagctgcatt aggaaagatt ttggtcgcaa aggataaaga gctgcaggag
     5341 cttgatattg aatttcacaa acttcagaat aaagctgacc atctcaggga gctttctgaa
     5401 aatattgatg attatgccga aggaataaaa tttctattac aggaagagaa gggaaaaaat
     5461 atttctacaa ttcttgatgc tatcgaagtg gaagataaat ttaaaattgc aatcgagacc
     5521 gcacttggtg agatttctaa ttacttgatc gtagatgatt caaaagatct gaa
//
LOCUS       DAORVQ010000209         5528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_9946_length_5528_cov_6.033071, whole genome shotgun sequence.
ACCESSION   DAORVQ010000209 DAORVQ010000000
VERSION     DAORVQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_5528_cov_6.033071"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1951
                     /locus_tag="PLX80_12730"
     CDS             332..1951
                     /locus_tag="PLX80_12730"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY21700.1"
                     /translation="MKKLFLVLILVFLSVGSHAQINTYPYVETGDNPIGWNPVQGIPI
                     WSLGTSYVNPAGYANDAALICNFFSYSPGPDGYVISPIMDFTTLTHPVIHFYVAYASY
                     NDNNDSMQFLVSTDFGNTFVDLPVAYRKSFNQSPSYATVPSQETAYSPNSTSHWRHET
                     IDLSAFAGQNYLLFAYRGVCAYGNNLFIDNFVVENADDYSTAVINSPGTYVLNELSFN
                     FNSIGQPASIKVLENDNPGGGLLQVTEHIDQVPVPSLASPQIKPNTTATTGNGSVFTP
                     DFIATDNWYTVSYTGDDLNGDANYDITIDISSFGSVSDPSRLYIVNRADYTSSWECLN
                     TVVTGNSLKASGLTSFSDFGVASSASLKLALKFYLEGFFNGTTMNADTVKVNLRSQTS
                     PYAIVDNSKTFLNFLGEGTLDFPNAISGTCYYFEVIHRNHIKVFSHSACEQLNNPVTP
                     YNFTNSISKTFGNNSIFVAGPNAGYASFTADVNQDGLVDLSDYSVIDNDAFNFYSGYI
                     PSDVNGDGVTELSDLTYCDNNTFNFVTAIVP"
     gene            2347..4419
                     /locus_tag="PLX80_12735"
     CDS             2347..4419
                     /locus_tag="PLX80_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21701.1"
                     /translation="MKKIVINLFLICLFVSLTGTSYSQSFKLEKVNQTDLTKINNPFD
                     AVSDGLNQLDGPNPPSTQAAIITPISGASTSANGRSPQSSTRYQRCYYFISADEMTSS
                     FFPVGSSITSLGFSYSVASFAAAADTFKVFFQNGPATFVDKGANWPTAIAPMTQVHQG
                     PISTIGAPGSIDYTLNNSGAFSYTGSGLWVAFEFQNITGTTGAANTALCNTVLPAPVL
                     RNMSAGSNVALNNTMSNSSNFRPVTRLGTASEDIVIVNNVIAMGEKINNCIDTNQYVT
                     NFTHLRPGIDTLTYKIVVKNVSSGFIKDSSSFTFILNDTGATTSYFITSSSLKTDSIK
                     TDSVIATVTAKSGEQIVGNNSGKTTTSSTYNTMNQAITSATNSGGAGFTNAGNLEIAI
                     AHNTGNCDVYISKVNYIFGSTGQPYLLKVYEGNVNTPGTLLYTSDTLTNSTGANSHTL
                     SSPVKSTSGKFFIALRQINTTNFSLQYQREIPVRRNVYYTGDGTPGDWGESLGGFKYN
                     VQVVTTVNLDLGLYLEGFFNGTTMIGDTVRVNLRSQSSPYPIIDTDQAYVNSTGNGTF
                     NFTNASPNTCYYYEVIHRNHIVTYSNASCNTLNDNTNSFNFKSAASQALGSNQVLVGS
                     SYSTYTADVNQDGVVDVTDYSITDNDANNFVAGYVVTDVNGDEVVDVADLGYCDNNAA
                     NFVGAIVP"
     gene            complement(4512..>5528)
                     /locus_tag="PLX80_12740"
     CDS             complement(4512..>5528)
                     /locus_tag="PLX80_12740"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HQY21702.1"
                     /translation="PSACGKTNLAMLTPTIPGWKVETVGDDIAWMKFGDDGRLYAINP
                     EYGFFGVAPGTSYHTNGNAMKSMESNSLFTNVALTDDGDVWWEGMTEEKPAHLKDWHG
                     KDWTPDSQTPSSHPNSRFTCPASQCPVIDKDWENPKGVPISAILFGGRRASIVPLVNE
                     AFDWQYGTFLGSSVSSEMTAAAAGTLGQLRHDPFAMLPFCGYNMGDYFAHWLKMGKKS
                     DSSKLPKIFYVNWFRKDENGKFIWPGYGDNIRVLKWVFERLEGSDNFEDRPYGRIPSK
                     GALDTSGLDISNDEMEKLFVISKEEGLSEVNELREYYKKFGDKLPAELADELNKLEER
                     YNKM"
BASE COUNT         1776 a          943 c          877 g         1932 t
ORIGIN      
        1 aaatttcatt tgaaaaaatc tgcattatgc ctgatacttt tatataaaaa tcctgcctgt
       61 ttaatatttt actttaagta aattaattat ctgctataat aatatattgg aaattaagta
      121 taaattttat aatttgtaca aataaaaata tgatttaact ttatttctgt aaagtattaa
      181 ttttttattc gaaattagta atttttgaat tcctaaccta aagtttagac gcaaaaaaat
      241 ttcatttaaa gggaaaaaat aaaaacgggt atttttatta aaaaaaatgt atccggattt
      301 ctttaaattt ttaaaaatta aattctgaaa aatgaaaaaa ttatttcttg ttttaattct
      361 agtctttcta agtgtcgggt cacatgctca gataaatacc tatccttatg tagaaaccgg
      421 tgataatcct attggatgga atccggttca gggaattcca atttggagcc ttggtacatc
      481 ttatgtcaat cctgcaggtt atgcaaatga tgcagcttta atttgcaatt ttttttccta
      541 ttctcccggt cctgatggtt atgttatttc accgataatg gattttacaa ctctgactca
      601 ccctgttatt catttttatg tagcatatgc ttcctataat gataataatg attcaatgca
      661 gtttcttgta tcaacagatt ttggaaatac ctttgttgac ttgccggttg cttacaggaa
      721 atcctttaat caatctcctt cttatgcaac tgttccatca caggaaacgg catatagtcc
      781 taattcaact tctcactgga gacatgaaac aatagacctt agtgcttttg ccggtcaaaa
      841 ttatcttctg tttgcttaca gaggtgtttg tgcttatgga aacaatttgt ttattgataa
      901 ctttgtagtt gagaatgctg atgactattc tactgctgtt attaattcac ccggaactta
      961 tgttttaaat gaactatcat ttaactttaa ttctattggt cagcctgctt ctataaaagt
     1021 ccttgaaaat gataatcccg gcggtggact tttacaggtt actgaacata ttgatcaggt
     1081 tcctgttccg tctcttgctt ctcctcaaat taaaccgaat acaactgcta ctaccggtaa
     1141 tggttctgtt tttactccgg attttatagc tactgataat tggtacactg tttcatatac
     1201 aggggatgac ttaaatggag atgcaaatta tgatattacg attgacattt catcatttgg
     1261 atcagtttca gatccatcaa gattatatat tgtaaataga gcagattata caagctcttg
     1321 ggaatgtctg aatacagttg tgactggaaa cagtttgaaa gcttccggac tcacttcatt
     1381 ttcagatttt ggagttgcaa gctctgcttc acttaagtta gcattgaaat tttatctcga
     1441 aggttttttt aatggaacaa caatgaacgc tgatactgta aaagttaatt tgagaagtca
     1501 gacatcacct tatgctattg ttgacaattc aaaaactttt ttaaattttt taggtgaagg
     1561 tactttagat tttccaaatg caatttcagg tacatgttac tattttgaag taattcacag
     1621 aaatcatata aaagtattca gtcattctgc ctgtgaacaa ttgaacaatc cagtaacacc
     1681 gtataatttt acaaattcaa tatctaaaac ttttggtaat aacagtatat ttgtagccgg
     1741 acctaatgca ggatatgctt cttttactgc tgatgtaaat caggacggat tagtagatct
     1801 ctcagattat tctgtaatag ataatgatgc ttttaatttt tattcaggtt acatcccctc
     1861 tgatgtaaac ggagacggag ttacagaact atcagactta acatattgtg ataataatac
     1921 ctttaatttt gtaactgcaa ttgttccata attttttttc aaataagtaa tttaaataat
     1981 ctgctccttt gttaatcgaa ggagcagttt tttgtataca atcaatttat gaaatttttt
     2041 aaaaattgaa aaagattttt tgaattttaa atttatttta ttttttgaaa ttattgagtt
     2101 gaaactttaa agtaattaac ttatggttaa aaaaattaaa agatatttcc gggattataa
     2161 aacttaagaa atttccaaga gtatcttttt gtgcaaaaaa agtttttatt aaaataaatt
     2221 tataaatata caaacaccaa ttaatttgat aggaggcaag ttagtggtga taacaacgac
     2281 tttattgaaa ttccaaatta tcattaaagt tcttaaaaaa cttttaataa cttaaattac
     2341 taaaaaatga aaaaaatcgt tatcaatctt ttccttatct gtttatttgt ttcgttaaca
     2401 ggaacttctt attctcagag ctttaagctt gagaaagtaa atcagacaga tcttacaaaa
     2461 attaataatc cgtttgacgc agtttcagac ggactaaatc agcttgacgg tccaaatccg
     2521 ccaagtacac aggcagcaat tattactccg atttctggtg cttcaacttc cgctaatgga
     2581 agatctccgc aatcttctac cagatatcag agatgttatt attttatttc tgctgatgaa
     2641 atgacatcat ctttctttcc agttggatca agcataacat cattaggatt ttcatattca
     2701 gtagcttctt ttgcagcagc agcagatact tttaaagtct ttttccaaaa tggacctgct
     2761 acttttgttg acaaaggagc taattggcca actgcaatag ctcctatgac tcaagtacat
     2821 caaggaccta tcagtacaat aggtgctccg ggttctattg attatacatt aaataattca
     2881 ggtgcatttt cctatacagg tagcggatta tgggtagctt ttgaatttca aaatattacc
     2941 ggtacaaccg gtgctgcaaa tactgcattg tgtaatacag ttttaccagc tcctgtatta
     3001 agaaatatgt ctgccggtag caatgttgct cttaataaca caatgtcgaa ttcttcaaat
     3061 ttcagacctg taacaagatt aggtactgca tcagaggata tagtaatagt caataatgta
     3121 atagctatgg gtgagaaaat aaataattgt atagatacaa atcagtatgt aacaaatttc
     3181 acacacctta gacctgggat tgatacttta acatataaga tagttgtaaa aaatgtttcc
     3241 agtggattta taaaggattc atcttcattt acttttattt taaatgatac tggtgcaact
     3301 acttcatatt ttataacatc aagcagttta aaaacagatt ctattaaaac agattcagta
     3361 attgcaactg ttacagcaaa atcaggtgaa caaatagtcg gaaataattc aggaaaaact
     3421 acaacttcat ctacatataa tacaatgaat caggcaatta cctctgctac aaattcaggt
     3481 ggagcaggat ttaccaacgc aggaaattta gagattgcaa tagctcataa tacaggaaat
     3541 tgtgatgtct atataagtaa agtaaattat atttttggat caaccgggca gccttatctt
     3601 ttaaaagtat atgaaggcaa tgtcaatact cccggtacat tattatatac ttccgataca
     3661 cttacgaatt ctaccggtgc taactctcat acactttcat ctcctgtaaa atcaacctcc
     3721 gggaaattct ttattgctct cagacaaatt aatacaacta atttttctct gcaataccag
     3781 cgggaaattc ctgtcagacg taatgtatat tatactggtg atggaacgcc gggagactgg
     3841 ggcgaatctt taggcggatt taaatacaat gttcaagtag taactactgt aaatctcgat
     3901 cttggattat atcttgaagg attctttaat ggtactacaa tgataggtga tactgtcaga
     3961 gtaaatctaa gaagccagtc atctccttat ccgatcattg atacagatca ggcttatgta
     4021 aattcaacag gtaacggtac atttaatttc acaaatgctt ctccaaatac atgttactat
     4081 tatgaagtaa ttcacagaaa tcatatagtt acttacagta atgcatcatg taatacactt
     4141 aatgataata ctaattcttt taatttcaaa agtgcagctt cacaggcatt gggaagtaac
     4201 caggtattag taggcagcag ttattctaca tacactgctg atgtaaatca ggacggagtt
     4261 gttgatgtta ctgattatag tattacagat aatgatgcaa ataattttgt agcaggttat
     4321 gttgtaacgg atgtaaatgg tgacgaagtt gttgacgtag ctgatcttgg ttattgcgat
     4381 aataatgcag ctaattttgt cggagcaatt gttccttaat tatgtgatta ttataaagtt
     4441 ataaaaatag aaagcaggga tcgaatttca atccctgctt ttattatgtt acaaataaat
     4501 ttttgaacca attacatttt attatatctt tcttcaagtt tattcaactc atctgccagc
     4561 tcagccggta acttatctcc gaacttttta taatattctc taagctcatt tacttccgac
     4621 aatccttctt cttttgaaat gacaaataat ttttccattt cgtcattact tatatcaagt
     4681 cccgaagtat ccaaagctcc ttttgaagga atccttccat aaggtctgtc ttcaaaatta
     4741 tccgaacctt ctaatctttc gaaaacccat tttaatactc tgatattatc accgtatcct
     4801 ggccagataa attttccgtt ctcatctttt ctaaaccagt taacataaaa tattttaggt
     4861 agttttgatg aatcactttt ctttcccatc ttcaaccagt gtgcgaaata atcacccata
     4921 ttgtatccgc aaaaaggcag catcgcaaat ggatcgtgtc ttaattgtcc taatgttccg
     4981 gcagcagctg ctgtcatttc tgaagatact gaagatccaa gaaatgttcc atattgccag
     5041 tcaaatgctt catttactaa tggcacaatt gaagctcttc taccgccaaa taatattgct
     5101 gagattggca ctcctttagg attttcccag tctttatcga tcacaggaca ttgtgaagcc
     5161 ggacaggtga atcttgaatt cggatgcgat gaaggagttt gagaatcagg tgtccagtct
     5221 ttaccatgcc agtctttaag atgtgcaggt ttttcttcag tcattccttc ccaccataca
     5281 tctccgtcat cagtcaaagc tacatttgta aataaagaat tactttccat agatttcatt
     5341 gcattgccgt ttgtgtgata tgaagttccg ggtgctactc caaagaatcc gtactcagga
     5401 ttgattgcat aaagtcttcc gtcatctcca aatttcatcc aggcaatatc atcaccgaca
     5461 gtttccactt tccagccggg aatagtagga gtcagcattg caagatttgt ctttccgcat
     5521 gcactcgg
//
LOCUS       DAORVQ010000210         5488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10066_length_5488_cov_6.701270, whole genome shotgun sequence.
ACCESSION   DAORVQ010000210 DAORVQ010000000
VERSION     DAORVQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_5488_cov_6.701270"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1103)
                     /locus_tag="PLX80_12745"
     CDS             complement(153..1103)
                     /locus_tag="PLX80_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HQY21703.1"
                     /translation="MKYRRLGNCGSNVSVIGFGSWLTIGGTVDKKTGIALIKSAFDNG
                     INFFDTADVYSLGESEKFLGNALKDFRRRDIFIASKCFWPMTPNINDKGLSRKHIFES
                     VNESLINLKTDYIDLYQCHRYDEETPVEETCRAFNSLIEQGKILYWGVSEWSKSNISE
                     AVNVCAKFNLHKPVSNQPQYSLLYRDIETNGVMDYCTKNGIGQVVWSPLGQGVLTGKY
                     NNKKAGKDTRLGHERYNVFVKKIASDENLKKVEKLIEIAKQLNTTASCLSLAWCLRKE
                     IISSTITSATKMNQLKENINAAELEVPADVIKKLDEIFKP"
     gene            complement(1182..2048)
                     /locus_tag="PLX80_12750"
     CDS             complement(1182..2048)
                     /locus_tag="PLX80_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21704.1"
                     /translation="MIKLVTMLIVLLCIKSSLAQFENTDKGARAIGLNGAYTSLSNNS
                     IAVFYNPSGLGQIKFREISAFYTPSIFGISGLTQTSFSYAEPLGFGTIGSGINSFGFD
                     LYREFNLLLSYGNVIREKVFLGFSVNYYNLSIQNYGSASSFGLDIGALAYITDFMRWG
                     FAAKNMTGSTIGISKEKIPQVYNTGLTFQPKEDLLFVIEAEKDVRYPLSFRSGFEYAV
                     FDQAEIRSGISTEPVSFTAGLGLSYGLFQLDYAINNHQDLGISHQGSITVNFGGPAAK
                     KFYRTQLVNAFK"
     gene            2140..3063
                     /locus_tag="PLX80_12755"
     CDS             2140..3063
                     /locus_tag="PLX80_12755"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HQY21705.1"
                     /translation="MQRQIKWFFIIIIFSFILKGKAYSQDKDFQSEVIVTYDLLAPEI
                     QQKLVLFQQKVQDYLNKNKYHDEKIDPIRVQWQFNFTGVNTINYNYQAKLFIASQRLV
                     YNPFDRNAQKYTTSFRFLDERCEFFYNESLPFVKNDQRFDPLLSLLDYYAYIVIGYDE
                     DSYNPKGGNKYFQKALDICNKVSVSLNGWNETGGGSKPSRLQLVQELLSVRFDHFRLG
                     YFEYMYLGLDSLATNKNNAYANILSALEVISNVKKREVRAFNVDIFFDSKAVEIAETF
                     IDYGDRNVYEKLMEYDRSHLSIYEEAKTRAR"
     gene            3242..4678
                     /locus_tag="PLX80_12760"
     CDS             3242..4678
                     /locus_tag="PLX80_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQY21706.1"
                     /translation="MRKLLLFFIAISLIISTNANAQFKNYNVKGGIQYSPTLPFSEYG
                     GAYSYTARAYLAFELSQLLAIELGAGFGQYKTNDDFNTPVLRSTSTENYTVSDVIPVD
                     VRLRISPMPSKNWNPYMYLGVGLLKYSTKELPAGYTNVADYNSFADGWTSTIPIGIGT
                     EIRMSDNVMLDISGGAYYSFSDQLNNFILGSWEDGGAQVSLGLTFAGGNDCETDNDKD
                     GLTKCREEQLGTNPNIADTDGDGLGDGEEVNTYMTNPLNKDTDGDTLQDGAEVKQHFT
                     DPLKNDTDGDKLMDNEEINTYLTLPTDSDTDDDNLMDGDEVKTYRTNPKMMDTDLGTI
                     QDGVEVGRGTDPLNPADDLPPAPPVEEPIKVGQVITLEGINFETNRADISSFAEDVLE
                     RALGYMKNNPNLEVEISGHTDNTGGLAHNMDLSLRRAESVKAWFVSNGISASRITTVG
                     YGPEQPVASNDTEEGRFQNRRIDFKRVK"
     gene            complement(4763..5458)
                     /locus_tag="PLX80_12765"
     CDS             complement(4763..5458)
                     /locus_tag="PLX80_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQY21707.1"
                     /translation="MNKKKIVVLTGAGISAESGLSTFRDSGGLWEGHNVMDVASPEGW
                     RRDRKLVLDFYNQRRRQLRNAVPNAAHLELVRLEEKYDVKIITQNVDDLHERAGSSEI
                     IHLHGELKKSQSSADPELIYGIEGDEINEGDKCELGSQLRPFIVWFGEMVPKMDEAVE
                     VALSADIFIVIGTSLVVYPAAGLLQFADVNIEKYLIDPSDPELFFNYSNLTHIKAKAT
                     TGTRELVDKLMID"
BASE COUNT         1790 a          986 c          932 g         1780 t
ORIGIN      
        1 tattcataac gggaattata ttatcagtct tatcagcaag cacagccata ctgatcactg
       61 aatacaagat cccgccgtag ctgtgaatta tatcaccgcc tgctttatga atgacatcaa
      121 cacaaggttc accgattata agatagttca ttttaaggtt taaatatttc gtctaatttt
      181 ttaatcacat cagccggaac ttcaagttct gcggcattaa tgttttcttt aagctgattc
      241 atttttgtag ctgaagttat tgtacttgaa ataatttctt ttctaagaca ccaggcaagt
      301 gaaagacatg aagctgtagt gtttaattgt tttgcgatct caataagctt ttccactttc
      361 ttaagatttt catcacttgc aatctttttt acaaatacat tatatctttc atgaccgagt
      421 cttgtatctt tacctgcttt tttattattg tattttccag tgagaactcc ctgtccaagc
      481 ggtgaccata ccacttgtcc gatcccgttt tttgtgcagt aatccattac gccgttagtt
      541 tcaatatctc tgtagagtaa actatactga ggctgattgc ttaccggttt atgcagattg
      601 aattttgcac agacatttac agcttctgat atattacttt ttgaccattc gcttactccc
      661 caataaagga tctttccctg ttctatcaga gagttaaaag ctctgcatgt ttcttccact
      721 ggagtttctt catcatatct gtgacactga tacaggtcaa tataatctgt tttaagattt
      781 atcagcgatt catttaccga ttcgaaaata tgttttcttg ataatccttt gtcattgata
      841 ttaggagtca tcggccagaa gcattttgat gctataaaaa tatctcttct tctgaaatcc
      901 tttaaagcat ttccgagaaa tttttcagac tcaccgagtg aatatacatc agcagtatcg
      961 aaaaaattta ttccgttgtc aaaagcagat tttattagag caatacctgt ctttttatca
     1021 actgtccctc ctatcgtcag ccagcttccg aatccgatta cgcttacgtt acttccgcaa
     1081 tttcctaatc ttctgtattt caaatataat ataatttatt ttaaatcaaa taacttattt
     1141 aggacagaaa attttttcaa atgaaaactg ccgtagatct attatttgaa tgcgttaact
     1201 aactgtgttc tataaaactt ctttgctgca ggtcctccaa aattcacagt gattgagccc
     1261 tgatgactta ttcccagatc ctgatgatta ttaattgcat aatctaattg aaacaatccg
     1321 taactcagtc cgagacctgc tgtaaaagaa accggttctg tgcttattcc tgatctgatt
     1381 tccgcctgat caaacacagc atattcaaaa cctgatctga atgacaaagg atatctcaca
     1441 tctttttctg cttcgattac aaacaacaga tcttcctttg gctgaaaggt cagtccggta
     1501 ttatagacct gaggaatttt ctcttttgaa attccaattg tagaacctgt catgtttttt
     1561 gctgcaaatc cccatctcat aaaatcagtg atatatgcaa gtgctccgat atccaaacca
     1621 aaagatgacg cagatccgta attctgaatg ctgagattat aataatttac actgaatccg
     1681 agaaaaactt tttcacgaat tacatttccg taagataaca gcagattaaa ttctctgtac
     1741 agatcaaatc cgaatgaatt tattcctgaa ccaattgttc caaaacccag tggttctgca
     1801 tagctaaatg aagtctgagt taaaccactt attccaaaaa ttgaaggggt ataaaaagct
     1861 gatatttccc tgaattttat ctgacctaac ccggaagggt tgtagaagac tgcaatagaa
     1921 ttatttgaaa gagaagtata agctccattt aatccaattg ctcttgctcc tttatccgta
     1981 ttttcgaact gtgccaagga acttttaata cataacaaca ctataagcat cgtgactaac
     2041 ttaatcatcg ttttccttta ataatttata aaagtgaatt gattatttga ttaggaacat
     2101 caaatttatc agaaataatt taatcgaaag agaaagaaaa tgcaaagaca aattaaatgg
     2161 ttttttatta tcataatatt ttcatttata cttaaaggaa aagcatattc acaggataag
     2221 gatttccagt cggaagtgat tgttacttat gatttgcttg ctccagaaat tcagcaaaag
     2281 cttgtactgt tccaacaaaa ggtacaggat tatctgaata agaataaata tcatgatgag
     2341 aagatcgacc caataagagt tcagtggcag tttaatttta caggagtaaa tacaataaat
     2401 tataactacc aggcaaaatt atttatagcc agccagagat tagtctataa tccatttgat
     2461 agaaacgctc agaaatatac aacatcattc aggtttttag atgagaggtg tgaatttttt
     2521 tataatgaat ctcttccttt tgtaaaaaat gatcagcgtt ttgatccatt actgagtctg
     2581 cttgattatt atgcttatat tgtaattggc tatgatgaag attcatataa tcccaaaggc
     2641 ggaaacaaat attttcaaaa agctttagat atctgtaaca aagtttcagt aagtcttaat
     2701 ggctggaacg aaactggcgg aggatctaag ccatcacgtc ttcagcttgt acaggaatta
     2761 ttaagcgttc gatttgatca tttcagatta ggatattttg aatatatgta tcttggtttg
     2821 gattcacttg cgaccaataa aaataatgca tatgcaaata ttttaagtgc acttgaagtt
     2881 atcagcaatg ttaaaaaaag agaagtcaga gcatttaatg ttgacatctt ttttgacagt
     2941 aaggcagttg agattgctga aacatttata gattacggag acagaaatgt ttatgagaaa
     3001 ctaatggagt atgacaggtc tcatttgtct atatatgaag aagctaaaac aagagcccgg
     3061 tgatgaaata gaatttatgg aaaccactta cattttctgt tttcaaacaa cttcacattt
     3121 cgcattatgt ttttttaaaa attattaata ttaatcagtt aaagtttagg ctttattaaa
     3181 tttcatgttt ggtattattt aactatttta gtattatttt ttaaataaaa cagttgataa
     3241 aatgagaaaa ctcttactat tttttattgc aatttcgttg atcatttcaa cgaatgcaaa
     3301 tgcacaattt aagaattaca atgtgaaagg cggtattcaa tacagcccta ccctaccgtt
     3361 ttctgaatac ggaggtgctt attcttatac agcaagagct tatctggcat tcgaattgtc
     3421 tcaattactc gcaattgagt taggtgcagg tttcgggcaa tacaaaacca acgatgattt
     3481 taacactccg gttttaagat ctaccagtac agaaaattac acagtatccg atgttattcc
     3541 tgtcgatgta aggcttagaa tttcaccaat gccttcaaag aactggaatc cttatatgta
     3601 cttaggagtc ggattattga aatattctac aaaagagctt cctgcaggat acactaatgt
     3661 tgctgattat aattcatttg ctgacggctg gacttcaaca attcctattg gaataggtac
     3721 tgaaataaga atgtctgaca atgtaatgct tgatatcagt ggcggtgcat attattcatt
     3781 ttcagatcag ttaaataatt ttatacttgg cagttgggaa gacggaggag cacaggtttc
     3841 acttggactt acatttgccg ggggaaacga ctgtgaaact gacaatgaca aagacggtct
     3901 gacaaaatgc cgtgaagaac agcttggaac caatccaaac attgcagata cagatggtga
     3961 cggattaggt gacggagaag aggtaaatac ctatatgacc aatccactga ataaagatac
     4021 agacggtgat actttacaag acggtgcaga ggtaaagcaa catttcactg atcctctgaa
     4081 aaatgatact gacggagata aactaatgga taatgaagaa attaatactt atctgacatt
     4141 accgacagat agtgacactg acgatgataa tctaatggac ggagatgaag ttaaaactta
     4201 caggacaaat cctaaaatga tggatacgga tcttggaaca attcaagatg gtgtagaagt
     4261 aggaagagga acagatcctt tgaatcctgc tgacgattta ccaccagcgc cacctgttga
     4321 agaaccaatt aaagtaggac aggttattac tctggaagga attaattttg aaacaaacag
     4381 agctgatatt tcttcatttg cagaagatgt actcgaaaga gcattagggt atatgaaaaa
     4441 taatcctaat cttgaagttg agatcagcgg ccatactgac aatacaggtg gtctggctca
     4501 taatatggat ctctctctca gaagagctga atctgtaaaa gcatggttcg tttcaaatgg
     4561 tatttcggct tcgagaatta ctaccgttgg ttacggtcct gaacagccgg tagcaagtaa
     4621 tgatacagaa gaaggaagat tccagaacag aaggatcgac ttcaagagag ttaaataatt
     4681 ccaataaaat aatattaaaa gctgtttttt cgaaaggaga aacagctttt ttttataaat
     4741 gaattactct ttcagatcaa attcaatcta tcattaattt atcaacaagt tctctagttc
     4801 ctgttgttgc cttagctttg atgtgtgtca gatttgaata attaaaaaat aattcaggat
     4861 cggaaggatc tattaaatat ttctcaatat tcacatctgc aaactgaaga agtcctgcag
     4921 cgggatacac tactaatgaa gtaccaatta caataaatat atcagcgctg agagccactt
     4981 ctacagcctc atccatcttt gggaccatct caccaaacca aacaatgaac ggccttagct
     5041 gtgatccaag ttcacattta tctccttcat ttatttcatc accttcaata ccatatatca
     5101 attcaggatc tgctgaactc tgcgactttt tgagttcacc atgtaaatga attatttcag
     5161 acgaaccggc tctttcatga agatcatcta cattctgtgt aattatttta acatcatatt
     5221 tttcttcaag ccggacaagt tctaaatgag ctgcattcgg aacagcattt ctaagctgtc
     5281 tgcgtctctg attataaaaa tccagtacaa gctttcggtc acgtctccag ccttccggcg
     5341 aagcaacgtc cattacatta tgaccttccc aaagtcctcc cgaatccctg aatgtagaaa
     5401 gaccgctttc tgcactgata ccggctccag tcaatacgac aattttcttt ttattcaaaa
     5461 cagatttata gttaatagtt aatagtta
//
LOCUS       DAORVQ010000211         5477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10099_length_5477_cov_5.281262, whole genome shotgun sequence.
ACCESSION   DAORVQ010000211 DAORVQ010000000
VERSION     DAORVQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10099_length_5477_cov_5.281262"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..91
                     /locus_tag="PLX80_12770"
     tRNA            3..91
                     /locus_tag="PLX80_12770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Ser,seq:gct)
     gene            complement(207..1175)
                     /locus_tag="PLX80_12775"
     CDS             complement(207..1175)
                     /locus_tag="PLX80_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21708.1"
                     /translation="MKKKIKIILSFLFCSVCVFTSVKTHSETIFIEAGNKGISPLNIN
                     AVVGDTVIWQKINDGEFQVICGGSYPGTSIPSGSPQINFLLNETQPSYSYILKVKGNY
                     NYKFIFNSFELTGKIYAGAPLPVELNDFVATTIKNEVILDWSTSGEINNDRFEVQRIE
                     ITDMIDYNPENLVFNTVGIIEGNGTSNEIHHYKFTDRNLSSGVYLYRLKQIDFNSNFL
                     FHILSDEINIGIPNRFIVNQNYPNPFNPSTKITFEIPEDGNVKVVIFSSKGSELKTLS
                     NSVIRGGYHSIDFNGSDISSGVYFYKIIYSSTNYVKSVTKKMLLLK"
     gene            complement(1187..2542)
                     /locus_tag="PLX80_12780"
     CDS             complement(1187..2542)
                     /locus_tag="PLX80_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21709.1"
                     /translation="MKSNYKVFPMSVLFILMLFIANSNLKSTTHTVTVESNMFTPPNL
                     NVSVGDTIKWQWLNGSHTTTCNGVFPGTSLPIGAAAWDSPMNSGNTVFMYPVTVAGNY
                     VYKCQPHAPDMGGTITASSGSGSILLTENFDYPVGDSLGAHGWVSFSGGSTNFLSVTS
                     PGLVYSGYRLSNIGNATTLTTSGQDAYTDFSSVDSTGDLYVSFMINLTSAQATAGYFE
                     AFLPPTSTTLYTARFYAKDVAGQYQFGLSKSTEGAGGIFYTTDSYNYGTTYLVVIKYK
                     RNPGTADDEMFAYVFNSGVPSTEPSTPNIGPVTGTATDGPIGRVALRQGTAASAPELR
                     IDGIQASKSWSDFATSITNYENIVSDSYELYQNYPNPFNPSTTIKFNIAEKGFVNLTI
                     FNALGQEIQTLVNDSKASGLYTVDFNGSNLNSGIYFYQLTFSGNNGNTYSETKKLLLI
                     K"
     gene            complement(2815..3942)
                     /locus_tag="PLX80_12785"
     CDS             complement(2815..3942)
                     /locus_tag="PLX80_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21710.1"
                     /translation="MKNILTLILCLFFTEISYSVNPEYILKAGNAVQTGANSLEFDLV
                     IIHTNSSNSTFEYAGGQYFFDFNLNISNGGALIYSIVDSDLPLHLQPRNPLVYTTGGE
                     MQLRLSTNVIPSPGQGYVISNLLTGTKLVRMKLMTNSGSFDVQYFSLRWRNGPENPYT
                     KIYSFENSVFTEITDPSNHSIDSLNNPLPVELASFIAEINDNNAELKWSTSNELNNSG
                     FEVERRDLKDQSFKKIGFLQGAGTISEMKNYSFTDKYLNTGSYKYRLKQLDFNGNFTY
                     FDLDQLVSINIPNAFKLEQNYPNPFNPETIIRYQLTAEKNVSLRIYDNLGKEVDQLVK
                     TIQKAGSYSVKFEGENLPGGVYFYRLEAGDYTDTKRMILIK"
     gene            complement(4027..5319)
                     /locus_tag="PLX80_12790"
     CDS             complement(4027..5319)
                     /locus_tag="PLX80_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21711.1"
                     /translation="MKKIILLFCFVFISSVSNSQLVVENFDYPLGDSLQQHNWVPFSG
                     AGFGLLVTSPGLTFPSYPGSGVGFATTLTLTNLSAQDAYRSFSSDITSGSVYTSFMVR
                     IDTATSLGNYFVSYLPSTSTSNYYGRVYAKTASNGNIAFGISKSSTNISIPEVYSDSV
                     YSKGVTYVVVVKYKFNTGTTTDDEVSLFVFNSALPNTEPAPNVGPITTTQTDVNNLGR
                     IALRQGLSGNAITPGAVVDGIRVTQSWKPSIWSFKLGIQGLFNGSTLNISDTTNIYVR
                     NNTSPYMVIDSATSIINSATLTGNYAFNFAPNGTYYFEVKYRKSPVYRNGISTWSKSG
                     GEPFTSSGGSYDFTTAASKAFGSNQILIGTTYNTYNGDVNQDDVVDLTDGSNIDNDAF
                     NFAGGYLNTDLTGDDVVDLGDATIADNNAGNFVSVIAP"
BASE COUNT         1869 a          906 c          851 g         1851 t
ORIGIN      
        1 ccggaagggt gggtgagtgg ctgaaaccaa cggtttgcta aaccgtcgta cgggtcaaac
       61 tgtaccgaga gttcgaatct ctccccttcc gctaatagtc tcaggattaa tattctgaga
      121 ctatttattt attataaact tatataaaaa aaagaactca ctttctatta ttagtgagtt
      181 cttaatattc tataccggta gattttttat ttcagcagca acattttttt tgtaactgac
      241 ttaacataat ttgtagaact ataaattatt ttataaaaat aaacaccact ggaaatgtca
      301 gagccgttaa aatcaataga atgataaccg cctcttatca cagaattact taatgtcttt
      361 agttcacttc cctttgatga aaaaattacc acttttacat ttccatcttc aggaatctca
      421 aatgttattt ttgtagacgg attaaatggg tttgggtaat tctgatttac aataaaacga
      481 tttggtatgc cgatattaat ttcatctgaa agtatgtgaa ataaaaagtt gctgttaaaa
      541 tcaatttgct ttaatctgta taaataaaca cctgatgaaa gatttctatc agtaaactta
      601 taatgatgaa tctcattaga agtaccattt ccttcaatta ttccaactgt attaaacacc
      661 aggttttcag gattataatc tatcatatct gtaatctcaa tcctttgaac ttcaaacctg
      721 tcattattaa tttcaccgct tgtactccag tccagtatga cttcattttt tatagtagta
      781 gctacaaagt catttaattc aaccggtaaa ggagcacctg catatatttt acctgtcagt
      841 tcaaaagaat taaaaataaa tttataatta taatttcctt tgactttcag aatataagaa
      901 tatgaaggtt gtgtttcatt caataaaaaa ttaatttgag gagaacctga aggaatactt
      961 gtacccggat aactaccgcc acatatcact tgaaattccc cgtcattgat cttttgccag
     1021 ataactgtat caccgacaac agcattaata tttaatggag aaataccttt atttcctgct
     1081 tcaataaaaa ttgtttcaga atgcgttttt acagaagtaa aaacgcaaac tgaacaaaat
     1141 aggaaactta atattatttt gattttcttt ttcaaaataa atcagtttat tttattaaca
     1201 ataatttttt tgtttcagaa tatgtatttc cattattacc agaaaaagtg agctgataga
     1261 aatagatgcc tgaattcaaa ttgcttccgt taaaatcaac tgtgtacaat cctgaagcct
     1321 tagaatcatt taccaaagtc tgaatttcct gtccaagcgc attgaaaatt gttagattta
     1381 caaatccttt ttctgcaata ttaaatttga tagttgttga agggttaaat ggatttggat
     1441 aattttgata aagttcataa ctatctgaaa ctatattttc ataattagtg attgaagtag
     1501 caaagtcact ccatgattta gatgcctgta ttccgtcaat ccttaattca ggagctgaag
     1561 ctgctgtacc ttgtcttaat gcaactctgc caattggtcc gtcagtagct gtaccggtaa
     1621 ccggtcctat atttggagtc gaaggctctg tggaaggaac accactgtta aaaacatatg
     1681 caaacatttc atcatctgca gtacccggat ttcttttata tttaattaca acaagataag
     1741 tagttccata attatacgaa tctgtagtgt agaaaatacc accagcacct tctgttgatt
     1801 tgctaagtcc gaactgatac tgacccgcta catctttagc ataaaatctt gcagtgtaaa
     1861 gtgttgtact ggtaggcgga aggaaagcct caaaataacc ggcagtagcc tgtgctgaag
     1921 ttaaattaat cataaaagaa acatacagat ctcctgttga atctacagaa ctaaaatctg
     1981 tatatgcgtc ttgtccgctt gtagtcagtg ttgttgcatt accaatgttt gataatctat
     2041 atccggaata taccagaccg ggactggtta cagataagaa atttgtagat cctccactga
     2101 atgaaaccca gccatgagca cctaaagaat caccaacagg ataatcaaaa ttttctgtca
     2161 gtaaaataga accactgccg gatgaagctg taatcgttcc tcccatatca ggagcatgtg
     2221 gctggcattt atatacataa tttcctgcta cagttacagg atacataaag acagtattac
     2281 cgctgttcat tggtgaatcc caagctgctg ctccaattgg cagagaagta ccagggaata
     2341 ccccgttaca tgtagttgtg tgagagccgt ttagccattg ccattttata gtatcaccaa
     2401 cacttacatt taaattagga ggtgtgaaca tgttgctttc gactgtaaca gtatgggtcg
     2461 tactttttaa attactgttc gcaataaaca gcatcagtat aaatagtact gacattggga
     2521 atactttgta attacttttc atttttttat tgggttaatt ttttaaatta ttttttgatt
     2581 tgataaaaat tttaaataaa attgctatat ccattttgaa aaaataaaat tgagataata
     2641 ataaggtgta ataaagaaaa gtatttataa gaacagatta aatcagaatt aattaaattt
     2701 gaatatagga ctgaatattt atataaaata ttctttgtca tattaaggaa aataaaacaa
     2761 attaccaatt catttttttg tattcgaaaa aaacttcata taactaaaga atatttattt
     2821 aataagtatc attcgtttag tatctgtgta atcccctgcc tcaagtctgt aaaaataaac
     2881 tcctcccggt aaattttcac cttcgaattt tacagaatat gatcccgctt tctgaattgt
     2941 ttttacaagc tgatctactt ctttcccaag gttatcatat atccttaaag aaacattttt
     3001 ctcagctgtt aactgataac gaattatagt ttcaggatta aatggattcg gataattctg
     3061 ctctagttta aaagcattgg gaatattaat actgactaac tgatccagat cgaaataagt
     3121 aaaatttccg ttgaaatcca gttgtttaag tctgtattta tatgaaccgg tattgagata
     3181 tttgtctgta aaagaataat ttttcatttc acttatagtt ccggctccct gaagaaaacc
     3241 aatcttttta aatgactgat cttttaaatc ccttctttca acttcaaaac cagaattatt
     3301 taattcattg gatgtactcc atttaagctc tgcattatta tcattaatct cagcaataaa
     3361 agaagccagt tccacaggta atggattatt aagcgaatct attgaatggt ttgaaggatc
     3421 tgtaatttct gtaaagacag aattttcaaa ggaataaatt tttgtataag gattttccgg
     3481 accgtttctc catcttaaag aaaaatactg cacatcaaaa gaacctgaat tagtcattag
     3541 tttcatcctt actaatttag taccggtaag aagattactg ataacatatc cctggcctgg
     3601 tgaaggaatg acattggtag aaagtcttag ctgcatttca cctcctgttg tataaacaag
     3661 cgggtttctg ggctgcagat gtaaaggaag atctgaatca acaatggaat agatcaatgc
     3721 tccaccatta ctgatattta aattgaaatc aaaaaaatac tgtcctcctg catattcaaa
     3781 agttgaattt gaactattag tatgaataat aactaaatca aactcaagag aatttgcacc
     3841 tgtctgaaca gcatttccgg ctttcagaat atattcagga tttacactgt aagaaatttc
     3901 tgtaaagaat aaacataaaa tcaatgttag aatatttttc atgcaagtcc tctcattcaa
     3961 attaaaaaga gtgagaatta aattattctc actctttata taaatttaga atttaaaaat
     4021 atttatttaa ggagcaatca cactaacgaa attacctgcg ttattatctg caattgttgc
     4081 atcgcctaaa tctacgacat catcaccggt aagatctgta ttcagataac cacctgcaaa
     4141 attaaatgca tcattatcta tgttagaacc gtctgtcaga tcaactacat catcctgatt
     4201 cacatctcca ttgtatgtat tataagtagt tcctataagt atctgattac tgccaaaagc
     4261 ttttgaagct gctgtagtaa aatcataact tccgcctgaa cttgtaaacg gttcaccacc
     4321 tgatttagac caggtagata ttccatttct gtaaacagga ctttttctgt attttacttc
     4381 aaaatagtat gttccgtttg gtgcaaaatt aaaagcataa tttcctgtca aagttgcaga
     4441 atttattatg gaagtagcag aatcaataac catatatggt gaagtattat tacgtacata
     4501 aatattagta gtatcactaa tattaagtgt gcttccatta aaaagtccct gtattccaag
     4561 tttgaagctc cagatagatg gcttccaaga ctgagttact ctgattccgt caactactgc
     4621 tcctggagtt atagcattgc ctgaaagtcc ctgtcttaaa gcaattcttc ctaaattatt
     4681 aacatcagtc tgagttgttg tgattggacc tacatttgga gcaggttcag tattaggtaa
     4741 agcagaattg aaaacaaata aacttacttc gtcatcggtt gttgtaccgg tattaaattt
     4801 atatttcact actactacat aagtaacacc tttggaataa actgaatctg aataaacttc
     4861 aggaattgat atgttagttg aacttttgct gataccaaaa gcaatatttc cgtttgatgc
     4921 tgttttagca tatactctgc cataatagtt tgatgtagaa gtagaaggca gataagatac
     4981 aaaataattg cctaatgatg ttgcagtatc aattcttacc ataaaggaag tataaacact
     5041 tccagaagta atatctgaac tgaatgatct gtaagcatcc tgagctgaca aattagtaag
     5101 agttaatgtt gtagcaaatc caactcctga acccggataa cttggaaatg taagtccggg
     5161 gcttgtcact aaaagaccaa atccggctcc ggaaaatggg acccaattat gctgctggag
     5221 tgaatcacca agcggataat cgaaattttc aactaccaac tgtgaatttg aaacagatga
     5281 tatgaaaaca aaacagaaaa gtaaaataat ttttttcatt gaggttaatt atttggttta
     5341 taaaataagt tatattgttg tttaaatttt gtttggattt atatacaact tataatttat
     5401 atgttaataa atcaataata gaaataataa tttattgtaa agttttgtaa aaaaattatt
     5461 gattttttaa gatattt
//
LOCUS       DAORVQ010000212         5475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10104_length_5475_cov_4.250738, whole genome shotgun sequence.
ACCESSION   DAORVQ010000212 DAORVQ010000000
VERSION     DAORVQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10104_length_5475_cov_4.250738"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLX80_12795"
     CDS             complement(<1..928)
                     /locus_tag="PLX80_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21712.1"
                     /translation="MKIFIFFAALTSIILTHQNSSANTSSSDTSYVLLSSDDYDYKNP
                     QFDKSGESVNYSVFDCILAYEKWSSETNSKIAVRFMNHNSLGPETELSDSLSLNIKPA
                     VAYKIVQLNPILNEPGAVVFQSNRNDNWDIYYSYYNSVNWSVPVQITSDTADETDPCI
                     VAYSTNDLSFMIAYSRGKDIYLKNYFNGAWQNEINITETDTDECFSPTMTRAQFTGGN
                     DFYIAYNRMNELSNKSIYYQRFSINTDGSVNNLQSRSVVQQNSQSNLRFSNGVNFTIL
                     NYDYDTLGRKSYFAAFIKFNGHDIRNQTYEYPG"
     gene            complement(1122..1685)
                     /locus_tag="PLX80_12800"
     CDS             complement(1122..1685)
                     /locus_tag="PLX80_12800"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HQY21713.1"
                     /translation="MKQNKFFVLAAFMLTSLFQIYVPAGIIADREILLSEGKEFKFKC
                     APVDPSDPFRGKYITLNFEENSFTTDNDNWDYNEDVYLTFKNNSEGFSEIESAAKEKP
                     ETGSDYLKLKTNGFSPEIKKLHINYPFDIYYLNETKAPKTEELYNSSLIDSNNVTYAI
                     IAIKDGEGAIKNLFINDKPIEEIIFED"
     gene            complement(1672..2970)
                     /locus_tag="PLX80_12805"
     CDS             complement(1672..2970)
                     /locus_tag="PLX80_12805"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HQY21714.1"
                     /translation="MSIQNELTELLENKIISEDTAEGIRNYYKGKAGKSENKLFIIFG
                     ILGALLTGLGIILIVAHNWDEFSLTVKTTFAFIPLITGQLICGFVLFKKIDSSVWRES
                     STVFLFFAVGTCISLISQIYHIEGELSSFILTWMALCIPLVYTMRSSATSLLYIIGIT
                     FYAVDTGYSGSNDIMSFIYWIMLMLIIPYYIMLIRRSENSNFLYIHNVFIPLSLIITL
                     GTISEDNSQIMYLAYMYLFSIFIIISNTNFSSDRVKSSGALSFFGISGILFILFVLSF
                     NSFWEDLSSASFSFNGIRSYTEIFASVILGITAFALLILRNRNRMSDLKFSTDIFEIT
                     FIIFTAIFIIGIYSDIAVVLINLLLFIIGIMTILKGIKLDHLGILNIGLLIISGIVIC
                     RFFDTDLSFIIRGILFVIVGLCFFGANLYLIKKRKTNEAK"
     gene            3230..>5475
                     /locus_tag="PLX80_12810"
     CDS             3230..>5475
                     /locus_tag="PLX80_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21715.1"
                     /translation="MVLNLEFTQKIITPDVPAFYYPRERLINKLISFKNKKVILITAP
                     AGYGKTSLSVEFFNKLKNEEKIWISISSYDNSIENFFLLLAMAFESNLKKSKFGARLK
                     SVLSRSQNSLFDEKVNDVISSFSSDLFSYLVKKKKDIYIFFDDFHNIDESDEVCTALN
                     YFLEFLPQNVHIIFISRREPTKINYPKFLAKNWLGKITKGDLSFSEQDIKKFIRLNKL
                     KTSKIDKNLLDEYIKSTEGWVTAIQLLLIRSEFNAIKNEDLVYGKSDVFEYFTYELFS
                     HLSPAEKNLLMTLTYPEYFNKHLIENVLEIKNGYKMLLDLYEKNIFINREDENFRFHE
                     LLRSYLRKKTEETFTREEITDLYIKLGSHYLKDKEWREDYIGLNYLILAGDYSKLKYW
                     IKMNASEKLLLIHSSGLYKKFDEISNEKFRNSLEFILLKVNTFVYKDKDIENALDYLK
                     QIIQLKFSIKSKDSILIPMNKIKRSDLDYYVEILMLICNCMFLKEGISKDNISISEHI
                     LNFKLKPEQEIQFTVSLIKSYIATGENSKSKKYILKLKKLFEKIVKIRNSNEKSIDEN
                     TFIESIFSMIIFFDYGDFKKGNEIVNFISNNIDIDRFDHSNLSQLCFALFTSYNNKKF
                     DQFFHQLKIKNTEKNKTIFSAYKNQYEFQNILNKFLNHQYKNVINDLEIMKQQTHLKN
                     YIYFIDALILYSYNLIENPGYVLRNTEKGDYSISVTRALILRQEAYLLNNDFDKYLKI
                     KKETTSIGT"
BASE COUNT         2039 a          787 c          817 g         1832 t
ORIGIN      
        1 ttccgggata ttcataggtt tgatttctta tatcatgccc attaaactta ataaaagctg
       61 caaagtaact ttttctcccc agtgtatcat aatcatagtt gagaattgta aaatttacac
      121 cgtttgaaaa tcttaaattg ctttgtgaat tttgttgaac aacagatctg ctttgtaaat
      181 tgttaacaga tccatcggta tttattgaga atctttgata atatattgat ttgttggaaa
      241 gttcattcat cctgttatat gcaatataaa aatcatttcc tccggtaaat tgagctcttg
      301 tcatggttgg agaaaaacat tcatcagtat ctgtttcagt tatattaatt tcattctgcc
      361 atgcaccgtt gaaataattt tttaaataaa tatctttacc tctgctgtaa gctatcataa
      421 aactcaaatc gtttgtagaa tatgcaacaa tacacggatc agtttcatca gcagtatctg
      481 atgtgatctg cacagggaca ctccagttta cagaattata atatgaataa tagatatccc
      541 agttatcatt tctgtttgac tgaaagacaa ctgcgcccgg ttcattaaga attggattta
      601 gctgaacaat tttataagca actgcaggct tgatattgag tgacaaacta tctgaaagtt
      661 cagtttcagg accgagagaa ttgtgattca taaacctgac agctatcttt gagttagttt
      721 cagaactcca tttttcatat gccaaaatac agtcaaatac tgaatagtta acggattccc
      781 cagacttatc aaactgtgga tttttataat cataatcatc agatgaaagc agtacatatg
      841 aagtgtcaga tgatgaagta tttgcagagg aattctgatg tgttaaaata atggaagtta
      901 aagctgcaaa aaaaataaag attttcataa ttgtaaattt taaattaata aaattaaaac
      961 tttaatttga aaaatcagac tatttaataa ggaaatgtct aaaatctatt catcgactgt
     1021 ctattataaa tatgatagaa aacgcataat tgaattatgt taaattaatt tatttcattt
     1081 aaaaaaacta atacttaaaa tgttaaaatg acaattcaaa attaatcttc aaaaattatc
     1141 tcttcaattg gtttatcatt tatgaataaa tttttaattg ctccttcacc atcctttatg
     1201 gctataattg cataagtgac gttattagaa tcaattaaag agctgttata gagttcctct
     1261 gtttttggtg ctttggtttc attgagatag tatatgtcaa atggataatt tatatgtaat
     1321 ttttttatct ccggactaaa accatttgtt tttagtttca gataatctga accggtttcg
     1381 ggtttttctt ttgcagcaga ctcaatttct gaaaatcctt cagaattatt tttaaatgtc
     1441 agatatacat cttcattata gtcccagttg tcattatcag ttgtgaaact attttcttca
     1501 aaatttagcg taatgtattt ccctctgaaa ggatcgcttg gatctacagg tgcgcatttg
     1561 aatttaaact cttttccttc cgagagtaaa atctccctgt cagctataat gcctgcaggt
     1621 acataaatct gaaataaaga tgttaacata aaagctgcta atacaaaaaa tttattttgc
     1681 ttcattagtt tttctttttt ttatcaaata taaatttgcc ccaaaaaagc aaagtccgac
     1741 tattacaaat aaaattcctc tgataataaa gctaaggtca gtatcgaaaa atctgcagat
     1801 cacaattccc gaaattataa gtaaaccaat gttcaatatt ccaagatgat caagcttgat
     1861 gccttttaat attgtcataa ttccgataat gaataacagc aggttgatca ggacaacggc
     1921 tatatctgaa taaattccaa taataaatat cgctgtaaaa ataataaatg tgatctcaaa
     1981 aatatcagtg ctgaatttaa gatctgacat tctatttcta tttcttaaga ttagtaaagc
     2041 aaatgcagtg ataccgagta ttactgatgc gaaaatttca gtataagatc tgattccatt
     2101 aaaactgaaa ctcgcagaag acagatcttc ccagaatgaa ttgaaactta aaacaaaaag
     2161 tataaaaagt attcccgata ttccaaagaa agataatgca cctgaacttt ttactctgtc
     2221 cgaagaaaaa tttgtatttg aaataataat aaaaatgctg aagagataca tgtatgctaa
     2281 atacattatt tgggaattgt cttcagatat tgttcctaat gtaatgatca gagataatgg
     2341 aataaaaaca ttatggatat agaggaaatt actattttca gatcttctga tcagcataat
     2401 gtaatagggg ataatcagca tcagcataat ccagtaaatg aaagacatta tatcattaga
     2461 tccggaatat cctgtatcca cagcgtagaa agtaataccg attatataaa gcagagaagt
     2521 cgcagacgat ctcatagtgt aaaccagtgg aatgcacagt gccatccagg taagtataaa
     2581 gctgctcagt tcaccttcga tatgatagat ctgactaatc agagaaatac atgtaccgac
     2641 agcaaaaaat aaaaatactg ttgagctttc ccgccagaca gaagaatcta tttttttgaa
     2701 taatacaaat ccgcatatca actgtccagt aataagagga atgaatgcaa aagttgtttt
     2761 tacagtcagg gaaaattcat cccagttatg agctacaatt aaaataatgc caagccccgt
     2821 gagtagtgca ccgagtattc cgaaaataat aaatagttta ttttcagact ttccggcttt
     2881 acctttgtaa taattgcgta ttccttctgc tgtatcttcc gaaataatct tattttccag
     2941 cagttcggtt aattcatttt gaatgctcat tatatataat agaaataaaa gaacgtaata
     3001 atacagaatt tataataaac tttcgctcca tagaataatc agataatact aatcaggtat
     3061 cacattaaaa tattatcttg aaattcaatt ttacatacat acagacaaat gccagttagc
     3121 aacagctgtt tataatttag aaataattga agtgaacttt gtaaattgtc tttaaataat
     3181 tagttaatta gattagtaat aagaatatga aaaactatag cagattaatt tggtattaaa
     3241 tctagaattt acacaaaaga tcataactcc cgatgtaccc gctttttatt atccaaggga
     3301 aaggctgatc aataaattaa tatcatttaa aaataaaaaa gtcatactga tcactgctcc
     3361 tgcaggatac ggaaaaacat ctctttcagt agagtttttt aataaattaa aaaatgaaga
     3421 aaaaatctgg atcagtatta gttcgtatga taattctata gaaaattttt ttttgcttct
     3481 tgcaatggct tttgaatcca atttgaaaaa atctaaattc ggagcaagat taaaatctgt
     3541 gctgtccaga tctcagaatt ctctttttga tgaaaaagta aatgatgtca tatcatcttt
     3601 ttcaagtgat ctcttttcat atttagtaaa aaagaaaaaa gacatttata tattttttga
     3661 tgattttcat aatattgatg aaagtgatga agtctgtact gcgttaaatt atttcctcga
     3721 gttccttcca cagaatgtgc atataatttt tatttcacga cgcgagccta ctaaaataaa
     3781 ttatcctaaa tttcttgcaa aaaactggct tgggaaaatt acaaaaggcg atctgtcttt
     3841 cagtgaacag gatataaaaa aatttataag attaaacaag ttaaagacca gcaaaattga
     3901 taagaattta cttgacgaat atataaaatc cacggaaggc tgggtaactg caatacagct
     3961 tcttttaatt cgaagtgaat ttaacgcgat taaaaatgaa gatcttgtct atggaaaatc
     4021 tgatgttttt gaatatttca catatgaatt attttcacat ttgtctcctg ctgaaaagaa
     4081 tcttttgatg actctgacct atcctgaata ttttaataag catttaatcg agaatgttct
     4141 agaaataaag aacggttaca aaatgctttt ggatctgtat gaaaaaaata ttttcataaa
     4201 cagggaagat gaaaacttca ggtttcatga attgctaaga agttaccttc gaaaaaaaac
     4261 tgaggaaaca tttacgagag aagaaatcac agatctttat attaaattag gatcccatta
     4321 tctaaaagac aaagaatggc gtgaggatta tattgggttg aactatctta ttcttgctgg
     4381 cgattattca aagctgaaat actggataaa aatgaatgcc tctgaaaaat tactattgat
     4441 acattcaagc ggtctttata aaaagtttga tgaaatttca aatgaaaaat ttagaaattc
     4501 acttgaattt attttactta aagttaatac gtttgtatat aaggacaaag atattgagaa
     4561 tgctctggat tatcttaaac aaataatcca gctcaaattt tccataaaaa gtaaagacag
     4621 tattctgatc cccatgaaca agataaaaag atctgatctt gattattatg ttgaaatact
     4681 tatgctgatt tgcaattgca tgtttttgaa agaaggtatt tcaaaagata acatcagtat
     4741 ttcagaacat atattgaatt ttaaattaaa acctgaacag gaaatacaat ttacagtatc
     4801 tcttattaaa tcttacattg cgacaggtga aaactctaag agcaagaaat acattttaaa
     4861 acttaaaaag ctctttgaga aaattgttaa aataagaaac agcaatgaaa aatctattga
     4921 tgaaaataca ttcatagaaa gtatcttttc tatgatcata ttttttgatt atggagattt
     4981 caaaaaaggt aatgagattg taaattttat ttcaaataat attgatatag atcggtttga
     5041 ccattcgaat ctgagtcagt tgtgttttgc attatttaca agttataata ataaaaaatt
     5101 cgatcagttc tttcatcagc taaaaatcaa gaatacagaa aaaaacaaaa caattttctc
     5161 ggcatacaaa aaccagtatg agtttcaaaa tatcttgaat aaatttttaa atcatcagta
     5221 taaaaatgtg ataaatgatc ttgagataat gaagcaacaa actcatttaa aaaattatat
     5281 ttattttatc gatgcattga tcctttattc atataatctt attgagaatc cgggttatgt
     5341 actgagaaat acggagaaag gggattacag tatttcagtt acacgggcat taatactgag
     5401 acaggaagca tatcttctaa acaatgattt cgataagtat ctgaaaatta aaaaagagac
     5461 cacttctatt gggac
//
LOCUS       DAORVQ010000213         5433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10238_length_5433_cov_6.202492, whole genome shotgun sequence.
ACCESSION   DAORVQ010000213 DAORVQ010000000
VERSION     DAORVQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10238_length_5433_cov_6.202492"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="PLX80_12815"
     CDS             <1..1781
                     /locus_tag="PLX80_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HQY21716.1"
                     /translation="RECFHGAHAIAPLKENILGVLSLIFWSLILIISIEYITIIMRAD
                     NDGEGGILALMSLLRRSKFKNTWIGSMVLLFGLFGAALLYGDSIITPAISVLAAMEGL
                     VVATPFFEPFIIPLTIIILIILFSVQKRGTSRIGKIFGPITFTWFIVIAILGILSIIE
                     NPEIFLSVNPYYAVNFFIENGFHGFVILGAVFLVVTGGEALYADMGHFGRKPIKITWF
                     ALVLPCLLLNYFGQGALLLRNPEAAINPFYILAPEWALYPLVILATFAAVIASQAVIS
                     GAFSLTFQALQLGYLPRMKIIHTSEDERGQIYIPQLNTMLFFATIALVISFKSSSNLA
                     SAYGIAVTTTMLMTTILLLITTIKLWHWNKIIAVIVVFFFIILDAAFWGANMLKIFQG
                     GWVPLAIGLVIYIIMKTWNNGRRKLLEKIKQQTTSLEYFVTEILSIRLIAIPGTAIYM
                     SSNKDGTPPPLIQNIKHNRLLHKQVIILTIMIQKVPHIKQDDRLEIQEPTEGFYRVLA
                     NYGYMDITNIQNIIELLKKNNIQLDMNKTTFFLGRETLIPGNEKGFNLLRNKLFILLS
                     NNAQRATEFFNIPPQRVFEVGTQVEL"
     gene            1802..2662
                     /locus_tag="PLX80_12820"
     CDS             1802..2662
                     /locus_tag="PLX80_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQY21717.1"
                     /translation="MSLISEFKQSLKTVDSEEILDLYFFRPISFVLVKLIYNTNITPN
                     HISIVALMFGIMSGVLYGFGTYKFLLLGSMSFFICNTLDCMDGQLARLKKNGTRIGRV
                     IDGFIDYITSVSVFLGLGIAMTHITGDALFSWTLTVTAGVSKALQNMFFDNYRNMYLE
                     YVYQKASGLDEEITEYSEEMDELEKVKGKFIEKLLISSYLSYSKFQKRSTKHFVLDVT
                     PEEYKRKNKTLLRVWSWIGSTTHMVALIIFSIFNRIDLYLISAITIGNLVFLILFLWQ
                     RSVINSFKKK"
     gene            2662..3399
                     /locus_tag="PLX80_12825"
     CDS             2662..3399
                     /locus_tag="PLX80_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HQY21718.1"
                     /translation="MQAVILAAGSSRRLRPLTDNTPKCLLNIRDKNLLHRTIENVTDY
                     GIDEFIFVTGYLENMIKDYLNENFPDLKMTFITNPGYENNNNSYSLWMTKDFVKNDIL
                     LLDSDILFDKKIIGKLLDSSHENCLAVNFTNDLDEEQIKVILDKKNKVLEIGKEISIS
                     KSAGESIGIEKFSTYFMKELFLILDRKISKENIINEFYEASFQEVIDNNDNSDQGNSI
                     YAEDVGMFGCMEIDTVSDYEKAQSIII"
     gene            3401..4333
                     /locus_tag="PLX80_12830"
     CDS             3401..4333
                     /locus_tag="PLX80_12830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY21719.1"
                     /translation="MNSYRRPGMFGGFTMFPPVIKILLVSNIAIYLIFNLILAGIQIE
                     GVPVDNFIIKYFALIPIDLGFYPWQLITYMFLHGGFFHLLLNMFALWMFGVELENMWG
                     QKRFMNYYLLCGIGAGLCNLLIAPLFTSVGPTVGASGAIYGILVAFGYLFPDRKIFIY
                     GIIPVKAKYLVIFYMLLEVFAVAGGTDSQIAHMAHLGGGVVGLIYMMIVFKKSGGFFD
                     TSSIKNKFNNLSKENKTYAPPVFSNGAKNKKENVPEAKYKELEVTDYKKEMETRQKDA
                     QDKIDAILDKLSAGGYQSLTEEEKKVLFQESKKL"
     gene            4355..>5433
                     /gene="ispG"
                     /locus_tag="PLX80_12835"
     CDS             4355..>5433
                     /gene="ispG"
                     /locus_tag="PLX80_12835"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HQY21720.1"
                     /translation="MQTETQSIKYCSSLTSYSRFITREVKIGDIPLGGNNPIRIQSMT
                     TTDTMDTIATVEQSIRIIEAGGEYVRITAPSINEAKNLENIKNELRKRGYKTPLIADI
                     HFTPNAAEVAARIVEKVRVNPGNYADRKKFETIDYSDDSYENELERIRKKFTPLVKIC
                     KEYGTAMRIGTNHGSLSDRIMSRFGDTPLGMVESALEFVRICEENDYHEIVLSMKASN
                     PKVMIEAYRLLINKMKLESMNYPLHLGVTEAGDGEDGRIKSAVGIGALLEDGIGDTIR
                     VSLTEEPEFEIPVCVDLVKRYSSRASEQIEIPPVIDNPLNPFDFKRRDTIEVYKVGGS
                     NVPVVFISANDEIRNGHSALKDLGY"
BASE COUNT         1814 a          872 c         1046 g         1701 t
ORIGIN      
        1 tgagggaatg ttttcatgga gcgcatgcaa ttgctcctct taaagaaaat atacttggag
       61 tattatcatt gattttctgg tctttgatac tgatcatctc aatagaatat attacaataa
      121 taatgagagc tgataacgac ggagaaggtg gtattcttgc tttgatgtca ttactcagaa
      181 gatctaaatt taagaataca tggataggtt caatggtact tttgtttgga cttttcggag
      241 cagctttact ttatggtgat agtattatta cacctgctat ttctgtgctg gcagcaatgg
      301 aaggattagt agttgcaacg ccgttctttg aaccattcat aattccactt acaataataa
      361 ttttaattat actttttagt gtacagaaaa gaggcaccag ccgaataggg aaaatttttg
      421 gtcccataac atttacctgg ttcattgtca ttgctatttt ggggatttta tcaataattg
      481 agaatcctga aatattttta tcagtaaatc cctattatgc agttaatttt tttattgaaa
      541 acggctttca cggatttgta atacttggtg ctgtatttct cgttgttaca ggtggtgaag
      601 cactttatgc agacatggga catttcggga gaaaacctat aaagatcaca tggtttgctt
      661 tggttttacc ttgtcttctg ttgaattatt ttggacaagg tgcgcttttg ctgagaaatc
      721 cggaagctgc aattaatcct ttttatattc ttgcaccgga atgggcattg tatccactgg
      781 tgatccttgc aacatttgct gctgttattg cttcccaggc tgttatttca ggagcttttt
      841 ctctgacatt tcaagctttg cagctcggat atctgccaag aatgaagatc atacatactt
      901 ctgaagatga aagaggacag atatatattc ctcaattaaa taccatgttg ttttttgcta
      961 caattgcatt ggtaataagt tttaaatcat caagtaatct cgcttcggcc tatggaattg
     1021 ccgttactac tacgatgctc atgactacaa tattactttt gataacaaca ataaagctct
     1081 ggcactggaa taaaataatt gctgtgatag ttgttttctt ttttataatt cttgatgcag
     1141 cattctgggg tgcaaatatg cttaagatat tccagggtgg ctgggtaccg ctggcaattg
     1201 gattagttat ttatattata atgaagacct ggaataatgg caggagaaag cttcttgaaa
     1261 agatcaaaca gcagactaca tcacttgaat attttgttac tgaaattctt agtatccgtt
     1321 taattgcaat tcccggaacg gcgatatata tgtcaagcaa caaggacgga actccgccac
     1381 cactgattca aaatataaaa cataacagac tcttacataa gcaggtgata attcttacta
     1441 ttatgatcca gaaagtacct catattaaac aggatgacag gcttgagatt caggaaccaa
     1501 ctgaaggatt ttacagagta ctggctaatt acggatacat ggatattacc aatattcaga
     1561 atataattga actactaaag aaaaataata ttcaactgga tatgaacaaa acaacattct
     1621 tccttggcag agaaactctg attcccggaa atgaaaaggg attcaatctg ttaagaaata
     1681 aactttttat attactttcc aataatgctc aaagggctac ggaatttttt aatatacccc
     1741 cgcaaagagt ctttgaagtc ggtacacagg tagaacttta ataaatataa ttttttataa
     1801 aatgtcatta atttctgaat ttaaacaatc tctcaaaaca gttgattcgg aagaaatact
     1861 tgacttatat tttttcagac caatatcatt tgtacttgta aaactgatct acaatactaa
     1921 tataactcct aatcatattt ccattgtagc tctgatgttt ggaataatgt ccggtgtttt
     1981 atacggcttt ggtacttata aatttttact tctaggttca atgagctttt tcatatgcaa
     2041 tacactggac tgcatggatg gtcagctcgc ccgacttaag aaaaacggta caaggatcgg
     2101 aagagtgatt gacggattta ttgattacat cacatcggta tctgtttttc tcggactagg
     2161 aattgcgatg actcatataa caggagatgc attattcagc tggactctta ctgtcacagc
     2221 aggagtatcg aaagctcttc agaatatgtt ctttgataat tacagaaata tgtatctcga
     2281 atatgtgtat cagaaagctt ccggattaga tgaagagatc actgaatatt cagaagagat
     2341 ggatgagctt gaaaaagtga aaggtaaatt tattgaaaaa ttacttatct catcttattt
     2401 atcttatagt aaatttcaaa aaagatcaac gaagcatttt gtccttgatg taactcctga
     2461 agaatacaaa agaaaaaata aaactctgct gagagtatgg agctggatcg gatcgactac
     2521 acacatggtt gctttgataa tcttctctat atttaacagg atagatcttt acttgatatc
     2581 tgccataaca ataggcaatc ttgttttcct tattctattt ctctggcaga gatcggtcat
     2641 taattcattt aagaaaaaat aatgcaggca gtaattttag cagcaggttc ctcaagacga
     2701 ttgagaccac ttacagataa tactccaaaa tgtcttctga atataagaga taaaaatctt
     2761 cttcacagga ctattgaaaa cgtaactgat tacggaattg acgaatttat ttttgtcaca
     2821 ggatatctgg aaaatatgat aaaagattat cttaatgaaa atttccccga tctcaaaatg
     2881 accttcatta caaatccggg ttatgaaaat aataataatt catattccct ttggatgaca
     2941 aaagattttg tgaaaaatga catactgctt ctcgacagtg atattctttt cgataaaaaa
     3001 attataggaa aattactgga tagcagtcat gaaaattgtc ttgcagtaaa tttcacaaat
     3061 gatctggatg aagagcagat caaagtaatt cttgataaga agaataaagt gcttgagata
     3121 ggaaaagaaa tttcgatcag caaatccgca ggtgaatcga taggaatcga aaagttttct
     3181 acatatttta tgaaagagct atttcttata ttagacagaa aaatttcaaa agaaaacatt
     3241 atcaatgagt tttatgaggc ttcatttcag gaagtaattg ataataatga taattcggat
     3301 caagggaaca gtatatatgc cgaagacgtt ggaatgttcg gctgcatgga aattgatact
     3361 gtcagtgatt acgaaaaagc ccaaagtata ataatttaaa atgaatagtt acagaagacc
     3421 cgggatgttc ggtggtttca ccatgttccc gccggtcata aagattttac tggtttcgaa
     3481 tatagcaatt tacctgatct tcaatttaat attggcaggt attcagatag aaggagttcc
     3541 agttgacaat tttatcataa aatatttcgc actgatccca attgatcttg gattctatcc
     3601 atggcaatta attacataca tgtttctgca tggcggattt ttccatttgt tgctgaatat
     3661 gtttgctctg tggatgtttg gcgttgagct tgagaatatg tggggacaga aaagatttat
     3721 gaattattat cttctttgtg gaattggtgc aggtctatgt aatctcctga ttgccccgct
     3781 ttttacatct gttggtccaa ctgtcggagc ttcgggagct atctacggaa tactcgttgc
     3841 attcggatat ctctttcctg acagaaaaat ttttatttac ggcatcattc ctgtaaaagc
     3901 gaaatacctg gtgatctttt atatgcttct tgaagtgttt gcagtcgcag gcggaacaga
     3961 cagtcagata gcgcacatgg cacatcttgg cggaggtgtc gttggactta tttatatgat
     4021 gattgtattt aaaaaatccg gaggtttctt tgatacttca tctataaaga ataaatttaa
     4081 taatctttca aaagagaata aaacatatgc acctccggtg tttagtaacg gagcaaagaa
     4141 taaaaaggaa aatgtacctg aagcaaaata taaagaactc gaagttacag attacaaaaa
     4201 ggaaatggaa accagacaga aagatgctca ggataaaatc gatgcaatat tagataagct
     4261 cagcgcaggc ggatatcaaa gcctgaccga agaggaaaag aaagtattat ttcaggaaag
     4321 taagaaatta taatttttta agtgtaatat aaatttgcaa acagaaaccc agtccataaa
     4381 atactgcagc agtttaactt cctattcaag atttatcaca cgtgaagtaa agatagggga
     4441 tattccatta ggtgggaata atccaatccg tattcagtca atgacgacta ctgatactat
     4501 ggatacaatt gcaactgtcg aacagtcgat cagaataatc gaagctggcg gcgaatatgt
     4561 ccgcattact gcaccgagca ttaatgaagc taagaattta gagaatataa aaaatgaact
     4621 tcgcaaaaga ggatacaaaa ctcctttgat agctgacatt catttcactc cgaatgcagc
     4681 cgaagtagct gcacgcattg tagagaaagt aagagtgaat ccggggaatt atgcagacag
     4741 aaaaaaattt gaaactatag attattcaga tgattcttat gaaaatgaac tggaaaggat
     4801 cagaaaaaaa ttcacaccgc ttgtaaagat atgtaaagaa tacggcactg caatgaggat
     4861 cggcacaaat cacggatcac tttctgacag gataatgagc cgcttcggtg atactcccct
     4921 tggaatggtc gaatctgcac ttgagtttgt aaggatctgt gaagaaaatg attatcacga
     4981 aattgtcctt tccatgaaag caagcaatcc aaaagtaatg atcgaagcat acagactttt
     5041 aataaataaa atgaagctgg agtctatgaa ctatccgctt cacctcggtg tgactgaggc
     5101 aggtgacggt gaagacggac gtataaaatc agccgtgggt ataggtgcat tactggaaga
     5161 cggtattggt gatactatca gagtttcact tactgaagaa cctgaatttg aaatacctgt
     5221 atgtgtcgat cttgtgaaaa gatattcttc aagagcatcg gagcaaatag aaattccacc
     5281 tgtcatagat aatccactga acccgtttga ttttaaaaga agagatacaa ttgaagttta
     5341 taaagtcggc ggcagtaatg ttcctgtagt ttttataagt gcaaatgatg aaattcgaaa
     5401 tggtcattca gctctaaaag atctcggata ttt
//
LOCUS       DAORVQ010000214         5431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10247_length_5431_cov_4.481027, whole genome shotgun sequence.
ACCESSION   DAORVQ010000214 DAORVQ010000000
VERSION     DAORVQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10247_length_5431_cov_4.481027"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLX80_12840"
     CDS             <1..790
                     /locus_tag="PLX80_12840"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQY21721.1"
                     /translation="GTLGIITKIYFKLIPLPKFRKVILVAFNSIEDSAKTVSKIFQSG
                     ITPAAIELMERSAIKAAEDQLGKKFPNTNAAAQLLIEVDGNYEESLDKDIESIAETVM
                     ENGAYDVILAEDNQKMNDLWALRRCIGEAVKSISEYKEEDTVVPRAKIPELIKGVKEI
                     SEKYGITTICYGHAGDGNVHVNILKDKMNETEWNENLDTAIREIFTHTVSLGGTISGE
                     HGIGYSQKSYLSIALGENEIALMKNIKKTLDPNNIMNPGKIFAE"
     gene            833..1237
                     /locus_tag="PLX80_12845"
     CDS             833..1237
                     /locus_tag="PLX80_12845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQY21722.1"
                     /translation="MINTETDLRVLYAHTDKMGVVNNVRYLEYFEAGRNDFLRKLGFP
                     YTELEKINIGLPVIEVFVKFISSAKYDDVIIIKTFLKKIPTVRFTIDYEIYCDDKLIV
                     TGYSTHSFVNLEKMKPVRPPEIFMELLRSKFS"
     gene            1271..2149
                     /gene="nadC"
                     /locus_tag="PLX80_12850"
     CDS             1271..2149
                     /gene="nadC"
                     /locus_tag="PLX80_12850"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQY21723.1"
                     /translation="MKKLPDSYTDFNFKKAVKLIKLAVKEDIGDGDVTSELLIKDSSV
                     SSAEILIKENGIAAGINIFNLVFNIIDKNVKTGFIVNDGDKIKKGTVIGYLKGNTASI
                     LKGERIALNILQRMSGIATFTNRLVRLLNNENIKIIDTRKTTPNFRLFEKLAVRIGGG
                     ENHRSGLYDMVLIKDNHIEACGGISGTLKKLEKSKKSKELKIEIEVKDLIEFNIILKS
                     GTKKIDRIMLDNFSVKDVIEAASLNKSKYEIEISGGVNESNIRKYKNIQNVNFISIGS
                     LTHSSKALDISLNFIT"
     gene            2204..2698
                     /locus_tag="PLX80_12855"
     CDS             2204..2698
                     /locus_tag="PLX80_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21724.1"
                     /translation="MTNFTNRFGFIFALFVSVIILFACGDKKESEVKKENTKEETTTT
                     TKENTKTSGSNQLYFVEEYSKEGEEIGKSEKFYINNKGGYITAMLRTSEPIGVGAVDV
                     RLERESLEGTEIIDTKPYDVFPEKSYFFFDKITFYKSGDYKVTVFKKDGSPIASGNVR
                     IDFK"
     gene            2731..3156
                     /locus_tag="PLX80_12860"
     CDS             2731..3156
                     /locus_tag="PLX80_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21725.1"
                     /translation="MKALKPLFFVFVLLVLFATKSESYAQQYPILYFCEVYGDNGEVG
                     VSDRFTTGYLTVMVKADGPLGLRDCSIQLDKYDRTTGKFSYYKKFPYAVSPDMKYVYF
                     AKNEESDMNFEDPGIYRVFLLDQNDRTVASGLVEIISKY"
     gene            3193..3600
                     /locus_tag="PLX80_12865"
     CDS             3193..3600
                     /locus_tag="PLX80_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21726.1"
                     /translation="MKKLFFITALLSVIFLSGTYCEVKSQSIQFCEDVDSEGMPVSES
                     TVFNISSKGGYLKFLVRLPYKISTSSVSYVIYKIDGDGNENYESTIYQDVEGSWSWFW
                     KEVTFYNSGVYNIAVYDGDNNYLTSGQISIQYY"
     gene            3712..5154
                     /locus_tag="PLX80_12870"
     CDS             3712..5154
                     /locus_tag="PLX80_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY21727.1"
                     /translation="MIEKIKSLSKDTLIYGTNTIVGRFLGFFLVPFYTNKFLPDEYGI
                     ITIIYSYIAILNVFFSIGLESGYMKFSSTLEVGSVKENFSNPYFIVIANSFFLSTLIY
                     LFAPELTGVFQVSQDYAYLIRYSALILFFDSIVLIPFAYLRLNNKAKSFAGIKIINIS
                     LNVILNIVLILYFNFGIEAVFISNLISSAVTFIMLIPVLRGIITFRFNKGLIKELIYF
                     SVPYIPAGIAANIVQIIDRPILKYLTDDKTVGIYTANYKLGIFMMLIVSMFEFAWRPF
                     FLNNAKDPDAKKLFSKILTVFVAFASVVFLFLTVFIDNIVKMDLPFGSHLIGDAYWSG
                     LEIVPVILFGYLFYGMYVNFMAGIYIEKKTKYLPLITGLGAAINVISNFILIPKLSFM
                     GAAISTLISYFVMMAGIYIVSQKFYKVNYEYKKIGIIFTSLISVTAIYFILNSYMEIN
                     WILKIVFPVIYILIIYFTGIFNKNVIKKFM"
BASE COUNT         1954 a          744 c          925 g         1808 t
ORIGIN      
        1 aggtacactt gggataatca cgaaaattta ttttaagctt attccattgc caaaattcag
       61 aaaagtaatt ttagttgctt tcaacagtat tgaagattca gcaaaaacag tttctaaaat
      121 atttcagtca gggataacac ctgctgcaat tgaacttatg gaaagatctg ccatcaaagc
      181 tgctgaagat cagcttggga aaaaatttcc aaatacaaat gcggcagctc agcttcttat
      241 tgaagtggat ggtaattatg aagaatcgct cgataaggat attgaatcca ttgcagaaac
      301 tgtaatggag aacggagctt acgatgtaat acttgcagaa gataatcaga aaatgaatga
      361 cctctgggct ttaagaagat gtatcggtga agctgtaaaa tcgatttctg aatataagga
      421 ggaagatact gttgtcccca gggcaaaaat acctgaactc ataaaaggcg ttaaagagat
      481 ctcagaaaaa tatggtatta caacaatatg ttatggtcat gctggtgacg gaaatgtaca
      541 tgtaaatatt ctcaaagata aaatgaacga aacggagtgg aatgaaaatc ttgatactgc
      601 tatcagggaa atttttactc atactgtttc actcggaggt actatctccg gcgagcacgg
      661 aataggctat tctcagaaat cttatctgtc aattgcactt ggagaaaacg aaattgcttt
      721 aatgaaaaat attaaaaaaa ctcttgatcc aaacaatatt atgaatccgg gtaagatatt
      781 tgcagagtaa ataattattc tgatttttaa aaagaaattt taaataaata aattgatcaa
      841 tactgaaact gaccttcgtg tattatatgc tcatactgat aaaatgggag tagtaaataa
      901 tgtcaggtat cttgaatatt ttgaagcggg aagaaatgac tttttgagga agttaggttt
      961 tccatataca gagcttgaaa aaattaatat agggctgccg gtaatagaag tttttgtaaa
     1021 atttatttct tctgctaaat acgatgatgt gataattata aaaacttttc ttaaaaaaat
     1081 tccgacagtc agatttacta ttgattatga aatttattgt gatgataaat taattgtcac
     1141 cggatattca actcattcat ttgtcaatct tgaaaaaatg aaaccggtaa gacctcctga
     1201 aatttttatg gagctgctca gatcaaaatt cagttaagga aatttaaaat tattctataa
     1261 attaattttt atgaaaaagt taccggacag ctacacagat ttcaatttta aaaaagcagt
     1321 caaactcatt aaacttgctg taaaagaaga cattggagat ggtgatgtca cttctgaatt
     1381 gttaatcaaa gatagttcag tttcatctgc tgaaattctt ataaaagaaa atggaatagc
     1441 tgccggaata aatattttca atcttgtttt caatattata gataagaatg taaaaacagg
     1501 atttattgtg aatgatggtg acaagataaa aaaggggaca gtcattggat atttgaaagg
     1561 gaatacagca tccattttaa aaggagaaag aattgctttg aatatattac agaggatgag
     1621 cggaatagct actttcacca accggcttgt aaggcttttg aataatgaaa atattaagat
     1681 cattgatacg agaaaaacta ctccaaattt cagattgttt gaaaaacttg cggtaagaat
     1741 tggcggaggt gaaaatcaca gatcaggatt atatgacatg gtactaataa aagataacca
     1801 tattgaagct tgcggaggaa tttcaggtac gttaaaaaaa cttgaaaagt caaaaaaatc
     1861 taaggaactc aaaatagaga tcgaagtaaa agacctcatt gaatttaata ttattttaaa
     1921 atcaggtaca aaaaaaatag acagaataat gcttgataat ttttccgtta aagatgtgat
     1981 agaagcagcc agtctgaata agagtaaata tgaaattgag atatcagggg gagtaaatga
     2041 aagcaacatc agaaaatata aaaatataca aaatgtaaat tttatttcga taggttcatt
     2101 aactcattct tcaaaagcat tagatatttc gttaaatttt attacttgaa aatctatatt
     2161 gataatcata aatttggaaa atcattaatt aaaaaataat aacatgacta attttacaaa
     2221 caggtttgga tttatttttg ctttatttgt atctgtaatt atactctttg cttgcggaga
     2281 caaaaaagaa tcagaagtta aaaaagaaaa tacaaaagaa gagacaacaa caacgacaaa
     2341 agaaaataca aaaacttccg gaagtaacca gctttatttt gtagaagaat attcaaaaga
     2401 aggtgaagaa ataggaaaga gtgaaaagtt ttatatcaat aataaaggcg gatatatcac
     2461 tgcaatgctg agaacttctg aacccatcgg agttggcgca gttgatgtta gattagaaag
     2521 agaatctctg gaaggaacgg agatcataga tacaaaacca tacgatgtat ttcctgaaaa
     2581 aagttatttc ttttttgata agatcacatt ctacaaatca ggtgattata aagtaacagt
     2641 atttaaaaaa gacggttctc caatagcatc aggaaatgtc agaattgatt tcaaataaaa
     2701 taattacatt ccataaatta aaaaataaaa atgaaagcct taaaaccatt gttttttgtt
     2761 tttgtactac tagttttatt tgcaacaaaa tctgagtctt atgctcagca gtatcctatc
     2821 ctttatttct gtgaagtata cggagataac ggtgaagtag gagtaagtga cagatttaca
     2881 acaggatatt taacagtaat ggtcaaagca gatggtcctc tcggactcag agactgttca
     2941 attcagcttg ataaatatga tcgtacaacc ggaaaattca gctattacaa aaaatttcca
     3001 tacgctgtat ctccggatat gaagtatgtt tattttgcaa agaatgaaga gagtgatatg
     3061 aattttgaag atccgggtat ttacagggta tttttacttg atcagaatga caggactgta
     3121 gccagcggac tagttgagat catttctaaa tattaaatcg attactcatt taatttcaat
     3181 taaattgttt taatgaaaaa attattcttc atcacagctt tattatcggt aatattttta
     3241 tcgggtacat attgtgaagt aaaatcgcag tcaattcaat tctgcgaaga tgtggatagt
     3301 gaaggcatgc cggtctcaga aagtactgtt tttaatatca gttcaaaagg cggttacctg
     3361 aagtttctag tcagacttcc atataaaata tctacaagtt ctgtaagcta tgtcatttat
     3421 aaaatcgatg gagatggaaa tgaaaattat gaatctacta tttatcagga tgttgagggc
     3481 agctggtctt ggttttggaa agaagtaaca ttctataatt caggtgtata taatatagct
     3541 gtatatgatg gtgataataa ttatttaact tcaggtcaga taagtattca atattattga
     3601 catagtaaat tcattactca aaattttttt aaaggttatt ccgtttttcg gaataacctt
     3661 tgttttttaa aattgaaatt catataattc gttttattcg gttaaaatgt tatgatagaa
     3721 aaaataaaaa gtctttccaa agatacactc atatatggca caaataccat tgtaggaaga
     3781 tttcttggat tttttcttgt acctttttat acaaataaat tccttcctga tgaatatggt
     3841 attattacaa ttatctattc ttatattgct atcttaaatg tatttttttc tataggtctt
     3901 gaatcaggtt atatgaaatt ttcttcgact ctcgaagtag gctctgtaaa agaaaatttt
     3961 tcgaacccgt actttattgt aattgcaaat tctttctttc tctccacatt gatatacctg
     4021 tttgcacctg agctgacagg ggtttttcag gtaagtcagg attatgctta tctgatccgt
     4081 tattctgcgc tgatattatt ttttgatagc atagttttaa taccttttgc atatctcaga
     4141 ttaaataata aagcaaaatc atttgccgga ataaaaataa taaatatttc ccttaatgtt
     4201 attttgaata ttgtactgat cctttatttc aattttggaa tagaagcagt atttatcagt
     4261 aatctaattt catcagcagt cacatttatc atgttgatac cggtacttag agggattata
     4321 actttcagat ttaataaagg tctcataaag gaattgattt atttttctgt cccgtacatc
     4381 cctgccggaa tagcggcaaa tattgtgcag atcattgaca gaccgatttt gaaatatctg
     4441 actgacgata aaacggtcgg tatttatact gcaaattata agctcggaat atttatgatg
     4501 ctgatagtat ctatgttcga atttgcatgg agaccgttct tcctgaataa tgcaaaagat
     4561 cctgatgcca aaaaactttt ctcaaaaata ctgactgtct ttgttgcatt tgcttccgtg
     4621 gtatttttat tcctcactgt ttttatagac aatatagtga aaatggatct gcctttcgga
     4681 agtcatctga taggggatgc atactggtcc ggacttgaga tcgtgcctgt gatcttattc
     4741 ggatatttgt tttatggaat gtatgtgaat tttatggcgg gaatttacat tgaaaaaaaa
     4801 acaaagtatc tgccattgat cacaggactt ggtgctgcaa taaatgtaat ttcaaatttc
     4861 attcttattc caaagttgtc attcatggga gcggctattt ctactttgat aagttatttt
     4921 gtaatgatgg caggtatcta cattgtgtca cagaagtttt ataaagtaaa ttacgaatac
     4981 aaaaaaattg gcattatatt tacttcttta atttccgtaa ctgcaatata tttcatcctg
     5041 aattcttata tggaaattaa ctggatccta aaaattgtct tccctgtaat ttatattctg
     5101 attatttact tcaccgggat atttaataaa aacgtcataa agaaatttat gtgattgtct
     5161 gaacgatgat ttaaggatta acatgatttc ctgattattg aattcaactg aaaatctata
     5221 tctatttttt gtatcatttt atcaatcata ctaacagtca caaaaataat gacaaatgct
     5281 tttatgcctc aaagtctcaa agtctcaaag tctcaaagtc tcaaagcctc aaagtctcaa
     5341 agcctcaaag aaaaactttt ttaaaaaatt ctataattct tacttaaatt aagaaagata
     5401 ctcaatgctg tcaaaatttt tttttatatt t
//
LOCUS       DAORVQ010000215         5375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10431_length_5375_cov_5.931955, whole genome shotgun sequence.
ACCESSION   DAORVQ010000215 DAORVQ010000000
VERSION     DAORVQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10431_length_5375_cov_5.931955"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PLX80_12875"
     CDS             complement(<1..1795)
                     /locus_tag="PLX80_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HQY21728.1"
                     /translation="MNKRIYKVLITVCIMFIAARADSFMFWNQAGNFPGTPAGYIAFG
                     NSASLNITGSFTVEMWLNPVNSTNPNNQILCEKRNGTDNNGYTLYLNQGRVAIRTNTN
                     TRLIGKIVVQNNNWTHVCGTYNSNSNTFSVYVNGVLDTSVVIINAAPVANADSLRIGK
                     GNINSPYSGYIDEFRIWDRPLSQTLVDKYRRTTLGTSTGNYNLLELSLTFQDKDSEGT
                     DFSSFDWSGNNGTGKNIGVGILDQSERPLNTIAPNDCLELNGTDESLCGVDNSDVSPV
                     NNITMEAWIFPRTVSGIRGIINKGTIGGVNYGLRLNGNSLNAVINGNNNFNSFVNIQS
                     QQWSHVSFTYNSNTGAYAFFINGNKAGGGVNNMGTIINGTDSLIIGSIGAAGTYFNGF
                     IDEVRISNYVKTQNVINKFLYQSIDKGNKPNNTMVNVVYNLDGYAYDNAGNGPVLEFK
                     QGAEFAHCSTTDDQPVSPVNRSDINDYQDGYRINQSVKNIPASGISGTVVDSFEVCLD
                     TLISDVNIYLALNHSAEQELSISLVSPDGASVNIYQNHILTNNSDIIITIFDDQADSS
                     LNPIVKYTSFAPEVKPRNPLNTVFAGKMTAGI"
     gene            complement(1904..3685)
                     /locus_tag="PLX80_12880"
     CDS             complement(1904..3685)
                     /locus_tag="PLX80_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21729.1"
                     /translation="MKHLLTNSKVTILLIAVICFLFTDVNAQFQIRTYNLHCNNNPGL
                     DNARSIINNFEGGNTIAGFSYAGGCGIGIFDWMFLQLQPNGNQVFANLYGTIAEDKCN
                     SVVQTKVDSTYLLSGFMSDNSNNFIKKATMMNINRAGVFNYSKMIDDTVASTYNQGCI
                     DSAKKNVFAGWKDVYLNINSKRTEKILATKYSPSGIMDWGHIYYTYNGNSPAMSLDEA
                     MSVCYQNSDKSYGVATRTNIFSKKLNVWDIMITKLSVNGGVVWNKIYPFNIPTAHFYP
                     NTEPRKIIPMSDGGFVVVGFTNNHEETANDIIVLRVDAAGNLMWSSCYGNTNFLELGQ
                     SIVLDGNNLVITGSVKRSITTPDAYILKIPVTGGPAVWINVWQRNNNLGEGGFDLVRT
                     NTLGNPGYAVTGDAAFNVQDVFFWRTNTNGLIPGGQCNDLLNIERIVNDQYVKDIAMK
                     PRKIKDKYFTPAIQTPQVTNIVRCFESDSNGNGSNESDNNGTNNETVNGINVVDYALD
                     QNYPNPFNPVTKINYDIPVSGNVMLKVFNSAGKEVMTMVNEFKNPGRYEVVFNGSQLS
                     SGVYYYKISASGDGKEFTDIKKMILIK"
     gene            3938..5359
                     /locus_tag="PLX80_12885"
     CDS             3938..5359
                     /locus_tag="PLX80_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21730.1"
                     /translation="MLNIKLFEILRKLSPSEMKEFSDYLNSPLFNKRKILFDLFNVYK
                     KFHPSFDDKNNTKEKSFSKIFPKRKYNDTVFRNLNSLLLTHAEDYLSFKNYSSDKFRV
                     KDHLLSELNSKNIFSLFEKNFKEGVKILDETEERDTGFFIKEYILFMQKDLYNSFINK
                     FSKKDITIASENFMKFYIMKLMEIQNYILYECRILDIDNKLFINPDFIDNTLKLMPAG
                     IRNMPQVRIYYNALMLERKQSKKYYEELKSLLFKHGELLEKEKLYNKYIDLIDFIKRS
                     RKANDLGSLKEIFQLRKRIIEKGLMPENFITNMFFLNLVTSALRLKEFKWVFSFIENY
                     NKFLLPEHRNSIRDISYALYYFEEKEFGKALSYSAKVRYEENYFNLLIKNLTARIYFE
                     LNETDLLISHIASYRMYLSKNRTLSKKEISAHSAFINTLDKLLKIKEKKKFYKLDDIF
                     SKSANSDFISKNWIDEKVRELEE"
BASE COUNT         1726 a          984 c          798 g         1867 t
ORIGIN      
        1 atatacctgc tgtcatttta cctgcgaata ctgtattcaa aggatttctc ggctttacct
       61 ccggagcaaa tgaagtgtat tttacaatcg gatttaaact gctgtcagcc tgatcgtcaa
      121 atatagtaat tatgatatca gaattatttg taagaatatg attctgatat atatttacag
      181 aagctccgtc aggcgataca agagagatac ttaattcctg ttcagcactg tgattcaacg
      241 caagataaat atttacatca ctgataagtg tatcaagaca aacttcaaat gaatcaacta
      301 ctgttcctga aattccggat gcaggaatat tcttaaccga ctggttaatt ctgtatccgt
      361 cctgataatc gtttatgtct gatctgttta caggtgatac cggctgatca tctgtcgtgc
      421 tgcagtgagc aaactctgct ccctgtttaa attccagcac aggaccattg cctgcattgt
      481 catatgcata tccgtccaga ttataaacta cattcaccat tgtattattc ggtttgtttc
      541 ctttatcaat ggattgatac aaaaatttat ttatcacatt ctgtgtcttt acataatttg
      601 aaattcttac ctcatcaatg aatccgttaa agtaagttcc ggcagctcca atgcttccaa
      661 ttattaaact gtcagttccg ttaattatcg tacccatatt attcacaccg ccccccgctt
      721 tatttccatt tataaaaaaa gcataagcac cagtatttga attatatgta aaactaacat
      781 gcgaccactg ctgtgactga atatttacaa aagaattaaa attattattt ccgtttatta
      841 ctgcattcag ggaatttcca ttcaaacgca gtccatagtt tactcctcca atagttccct
      901 tatttataat accgcgtatg cctgaaacag tacgcggaaa tatccatgct tccatagtaa
      961 tattatttac aggactcaca tcagagttgt caacgccgca taaagattca tccgttccgt
     1021 taagttccag gcagtcatta ggagcaatag tattcaaagg tctttcactc tgatcaagaa
     1081 ttcctactcc gatatttttt ccggtaccgt tatttcccga ccagtcaaac gaactaaagt
     1141 ccgtaccttc agagtcctta tcctgaaaag taagagataa ttccagtaaa ttataattac
     1201 ccgtcgaagt accgagtgtt gtccttctgt atttatcaac caacgtctgt gataaaggtc
     1261 tgtcccagat tctgaattcg tcaatgtatc cgctataagg actgtttata ttcccttttc
     1321 caattcttaa agaatctgca tttgccacag gtgcagcatt tataattact acacttgtat
     1381 ctaaaactcc attcacataa actgaaaatg tattcgaatt tgaattatat gtgccgcaga
     1441 catgtgtcca gttattattt tgtacaacga tttttccaat aagtcttgta ttagtatttg
     1501 tcctgatcgc cactcttccc tgattgagat ataaagtata tccattatta tctgtaccgt
     1561 ttctcttttc acaaaggatc tgattattcg gattggtaga atttacagga ttcagccaca
     1621 tctcaactgt aaaacttcct gtaatattca gtgatgcaga atttccgaat gcaatgtatc
     1681 ctgccggtgt tcccggaaaa tttcccgcct gattccagaa cataaatgaa tctgctctgg
     1741 ctgctataaa cattatgcaa actgttatta aaactttgta tattctttta ttcatttttt
     1801 caggttttaa attaacttcc taaaataact atttataact taaatttcac aatgcaaaaa
     1861 aggcagagat attatctccg ccttttgctt aagttaattt atcttatttt ataaggatca
     1921 ttttctttat atctgtgaat tctttcccgt caccgcttgc agagatcttg taataataga
     1981 ctccggatga aagctgggaa ccgttgaata ctacttcata tcttccagga ttcttgaatt
     2041 catttaccat tgtcattact tcttttcctg cactgttaaa tactttcagc attacattac
     2101 ccgaaacagg aatgtcataa ttaatttttg taacaggatt aaaaggattt ggataatttt
     2161 gatcaagagc gtaatctact acattaattc cgtttactgt ttcattgtta gttccgttgt
     2221 tatctgattc attagatcca ttaccatttg aatctgattc aaaacatctc actatatttg
     2281 tcacctgtgg agtttgaatt gccggtgtaa aatatttatc ttttatcttt cttggtttca
     2341 tagctatatc ttttacatat tgatcattca caattctttc tatgttaagt aaatcattac
     2401 attgacctcc gggaattaaa ccgtttgtat ttgttctcca gaaaaaaaca tcctgtacat
     2461 taaaagcagc atcaccagtt actgcatatc ccgggttacc aagagtattt gttcttacca
     2521 gatcaaatcc gccttcacca agattattat ttctttgcca gacatttatc cagactgccg
     2581 gtccgcctgt tacagggatt ttcaatatgt atgcatcagg tgtagtaata gatcttttga
     2641 ctgaacctgt aattacaaga ttatttccat cgagtactat tgactgacca agctccagga
     2701 aatttgtatt tccataacag cttgaccaca tcagatttcc ggctgcatcg actcttaata
     2761 cgataatatc atttgctgtt tcttcatgat tatttgtaaa cccaactact acaaatccgc
     2821 cgtcactcat tggaatgatc tttcttggct cagtattcgg ataaaaatga gcagtcggaa
     2881 tattgaaagg ataaatttta ttccatacca ctccgccgtt tacactgagt tttgtgatca
     2941 ttatatccca tacgttgagt ttctttgaga aaatatttgt cctcgttgca acaccataac
     3001 ttttatctga attttgataa catacactca ttgcctcatc taaactcatt gcaggagaat
     3061 ttccattgta agtataataa atatgtcccc agtccattat tcctgaagga gaatatttag
     3121 ttgctaatat tttttctgtc ctctttgaat taatatttaa ataaacatcc ttccatcctg
     3181 caaatacatt cttctttgcc gaatcaatac atccctgatt ataagtactt gcaactgtat
     3241 catcaatcat tttagaataa ttaaatacac ctgctctgtt gatgttcatc atagttgctt
     3301 tctttataaa gttattggaa ttatcagaca taaaacctga cagcagataa gtggaatcta
     3361 cttttgtctg tactacggaa ttacatttat cttcggcaat tgtcccgtaa agatttgcaa
     3421 aaacctgatt tccgtttggc tgtaactgca gaaacatcca gtcaaaaatt cctattccgc
     3481 agccacctgc atatgaaaat ccggctatag tatttcctcc ttcaaaattg ttgatgatcg
     3541 atcttgcatt atcgagaccc ggattattgt tgcagtgaag attgtaagtt ctgatctgaa
     3601 actgtgcgtt tacatctgtg aataaaaaac agataactgc gattagcaga attgttactt
     3661 ttgagtttgt tagtaaatgt ttcattttta atttcctttc ggttaagttt ttaaaagttt
     3721 ttaaagtaaa cttgttaatt aatttctgta caaagttact cacttttcgc actgccgaac
     3781 aatcaaacaa tttccgtttt gtttcaaaat aattctctta attggtttta atattccatt
     3841 gaaatgctta atttcaaatc agaatcaaag gtttttgcta aatgaattgt gtcacgatat
     3901 cttttaataa attgcatcaa atcagaccga aaattatatg ctaaacatca aattatttga
     3961 aattctcaga aaactttcac cttctgaaat gaaagaattt tctgattatc tgaattcacc
     4021 gctgtttaat aaaagaaaaa ttctctttga tctgtttaat gtatataaga aatttcatcc
     4081 atcatttgat gataagaaca ataccaaaga aaaatctttc agtaagatct ttccgaaaag
     4141 aaaatacaat gatactgttt tcagaaatct taactcactt cttctgacac atgctgagga
     4201 ttatttatca tttaagaatt acagcagtga taaatttaga gtgaaggatc atttactttc
     4261 cgaacttaac tcaaaaaata ttttttcatt atttgaaaaa aatttcaagg aaggtgtgaa
     4321 gattcttgat gagacagaag aaagggatac agggtttttt ataaaggagt atattctttt
     4381 tatgcaaaaa gatctttaca atagttttat aaataaattt tcaaaaaagg atattaccat
     4441 tgcttccgag aactttatga aattttatat aatgaagctg atggaaattc agaattatat
     4501 tctttatgaa tgcagaatac tggacattga taataaactt tttataaacc ctgacttcat
     4561 agacaacaca ctaaaactga tgccggcagg gataagaaat atgccgcagg tgagaattta
     4621 ttataatgct ttaatgctcg agagaaagca aagtaaaaaa tattatgaag agttaaaatc
     4681 tttgttattc aagcacggag agttacttga aaaggaaaag ctgtataata aatatattga
     4741 tctcatagat tttataaaaa gatcgcgtaa ggcaaatgat ctcgggtcat tgaaagaaat
     4801 atttcaactg agaaaaagga taattgagaa aggtctgatg cctgagaatt ttattacaaa
     4861 tatgttcttc ctgaatcttg tcacctcagc tctaagactt aaggaattta aatgggtatt
     4921 ttcatttata gaaaattaca acaaatttct tttacccgag cacagaaatt ctattagaga
     4981 tatttcttat gctttatatt attttgagga aaaggaattt ggcaaagctc tttcatattc
     5041 cgctaaagtc agatatgagg aaaattattt caacctttta ataaaaaatc ttacagcccg
     5101 aatttatttc gaactgaatg aaaccgatct tctcatcagc cacattgcat catacagaat
     5161 gtatctttca aaaaaccgta cactttcgaa aaaagaaatt tcagctcatt cagcatttat
     5221 taacactctc gacaaacttc tgaaaataaa agagaagaaa aagttttata agctggatga
     5281 catattttca aaatctgcaa attcagattt catcagcaaa aactggattg atgaaaaagt
     5341 ccgtgaactg gaagagtgat caattttaat ggtcc
//
LOCUS       DAORVQ010000216         5353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10494_length_5353_cov_6.848245, whole genome shotgun sequence.
ACCESSION   DAORVQ010000216 DAORVQ010000000
VERSION     DAORVQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10494_length_5353_cov_6.848245"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLX80_12890"
     CDS             <1..641
                     /locus_tag="PLX80_12890"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQY21731.1"
                     /translation="KSETNLNTPERNIILKDAYVEIPILEIPVIENQIQVPLKDLSAL
                     TPEPVAKRNIKEDVTLKNQSELENVKLPVSSEGTEDPSEVTAEVPGKIKIDEVKIDES
                     IEKIKEPVKTPFEEFQVEKSPSALNLNSIKASMKYPSIALSTGTEGRVTVRVLVGTDG
                     SVIKIGSISGPEVFRDEVKENVMGLQFTPAMMNDQTVKCWVSVPFSFKLNNK"
     gene            complement(942..1343)
                     /locus_tag="PLX80_12895"
     CDS             complement(942..1343)
                     /locus_tag="PLX80_12895"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY21732.1"
                     /translation="MEKSSKPEFFNLRPEIEKAYGYTHAVRIGNDIKVSGAVSMDDSG
                     NPTAVGDLQLQMKNCYSDLEKVLKHFGCTFDDVVVENIFTTNMPEFLIQSEYRNSIYT
                     KQFPAGSWLGVKELALPEFLIEIELEVHVSN"
     gene            complement(1422..2234)
                     /locus_tag="PLX80_12900"
     CDS             complement(1422..2234)
                     /locus_tag="PLX80_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21733.1"
                     /translation="MKRKSDPTNIDKLDFTSNKINLCAYFYKGSGSKKKPTLIWLQGN
                     PGGPEDGTNPWSIELSKKGVNVFRFNYEGLWGNDGEFNFSNSLINLKSVLDFLHNKVN
                     TDLYQIDTNNLIFGGFSFGSSVVFVGALYDTRIKNIVAVSLCDHGYFGRQFMDPYSKI
                     REFLEETAKNIFLPNGVVNQEKEVFVKDLTDNICRYDFVRHSKKLLKKKILIICGYND
                     EICPVEDHLLPLYRKLKELGHKDLKVIINDCAHVQEIDLAEVFINRIKDTKS"
     gene            complement(2447..3493)
                     /locus_tag="PLX80_12905"
     CDS             complement(2447..3493)
                     /locus_tag="PLX80_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21734.1"
                     /translation="MSKLKSFALLIFSLLILTGCSFISNIKEKLISGGKKISEKVSTS
                     DKTSEEDLKFYNKYIEVMNKIQGQGENIYKYYKREVPPPGQINKNSYILNMSMQMECQ
                     FLGNTVKDYQRSLLDGGELSKLKASDEMQKTLESDLKNLLPVLEEYYRVSYRVAEYYF
                     KREYVNDMSKIVPYDKDMENVYNKYKTEFDKFAADLKKYKPARIIYDPSSVSDPDEAS
                     AQTMLNVYGDILESAEGFYESIEKIEYKSDLSVPETKFNEFEKTVRESKSTVMKASFG
                     ETVKFMKYHYEDYFAPSAESFINAGKVFFGSAPKSKSEDEFNEKFNELIQSYNKMINS
                     YNTSVETINRVRSW"
     gene            complement(3712..4755)
                     /locus_tag="PLX80_12910"
     CDS             complement(3712..4755)
                     /locus_tag="PLX80_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY21735.1"
                     /translation="MMAKILVADDETDLEILIKQKFRQKIREHEYEFVFAVNGNDALE
                     KLKQHTDIDIVLTDINMPEMDGLTLLSKLNVSNPLLKSVIVSAYGDMENIRTAMNRGA
                     FDFITKPINFEDLAVTMEKTINQVLQIKNTLKAIKENNILKMYVDETVLNFMDSREFE
                     TSLMANETIEASVVFIDICSFTTISEIEPPDTVVKLLNSYFDIIVKEIIGQGGYVDKF
                     IGDAIMAVFRGDYHLDRAVDASLAVRNQIEKLPALSKSVNFNPKVSIGLNCGEMISGN
                     IGSANLKRLDYTVIGDTVNTAQRIQSTAEPGQILLSEPAYEEIKESFNCRKVGEVILK
                     NKSIPVNVYEVLD"
     gene            complement(4757..5131)
                     /locus_tag="PLX80_12915"
     CDS             complement(4757..5131)
                     /locus_tag="PLX80_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY21736.1"
                     /translation="MKILVVDDEKDIQTLFEQRFRKEIKDKIVEFAFTFSGEEAIEYL
                     SLHELEHEAILILSDINMPGMSGLELLEHIKKKYHKPPPVVMMITAYGDGENFKTAQE
                     LGADDFLTKPVDFKLLKEKLKI"
     gene            complement(5141..>5353)
                     /locus_tag="PLX80_12920"
     CDS             complement(5141..>5353)
                     /locus_tag="PLX80_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY21737.1"
                     /translation="RVKDNGNGIPQKVMDKIFQPFFTTKPTGQGTGLGLSLSYDIVKA
                     HGGELKVETKSGEGTEFSVVLPYLKN"
BASE COUNT         1668 a          963 c          822 g         1900 t
ORIGIN      
        1 ataaatcgga gacaaactta aatactcccg aaagaaatat tatacttaaa gatgcatatg
       61 ttgagatacc gatactggaa attccggtaa tagaaaatca gatacaggtt cctttgaaag
      121 atctttcggc attgactccg gagcccgtcg caaaaagaaa tataaaagaa gatgtaacat
      181 taaaaaatca gagtgaactt gaaaatgtaa aactgccggt atcatctgaa ggaactgaag
      241 atccgtcaga agtcacagca gaagttccgg gtaaaataaa aatagatgaa gtaaaaattg
      301 atgagagtat tgagaagatt aaagaaccgg taaaaacacc ttttgaagaa tttcaggtag
      361 aaaaatcacc gtcagcatta aatttaaatt caataaaagc atcaatgaaa tatccttcga
      421 ttgcactttc cacagggaca gaaggaagag ttacagtcag agtgttggtc ggcacagacg
      481 gttcagtaat taaaattgga agcataagcg gtccggaagt atttcgagat gaggtaaaag
      541 agaatgtaat gggacttcag ttcacaccgg ctatgatgaa cgatcagact gtaaaatgct
      601 gggtaagtgt tccgtttagt tttaaattga ataataaata aaaattgttt tttaaaaata
      661 tccactcctt cctgagctgt atctcgggaa ggagtttttt tgtaatcaat tttaagttat
      721 aaaactttgc cgttaccgga gttccaaaag tttgaagagt ttaatgttca gaataaattt
      781 gtaagtagtt caaccttttt tagtctccaa caacaatgtc attagctgat cgtgctgttc
      841 tctcaaatta ttggtgcctg aaagtctgag ttcagttata tagtattcta tacacagtgt
      901 ttgtcaaatt ttcaggaatt ccaacaatgt tgaaattaat ttcaattcga aacatgaact
      961 tcaagttcta tctcaattaa aaattccggt aatgccaatt ccttaactcc aagccatgag
     1021 cctgcaggga attgttttgt gtaaattgaa ttcctgtatt cggattgaat cagaaattca
     1081 ggcatattag ttgtaaatat attttcaaca accacatcgt caaatgtaca gccaaaatgc
     1141 tttaatactt tttccagatc agaataacag ttcttcatct gcagctgaag atctcccaca
     1201 gctgttggat taccggaatc atccatgctg actgcacctg aaacttttat atcattgccg
     1261 attcttacgg catgcgtgta tccataagct ttttcaattt ccggtcggag gttgaaaaac
     1321 tcaggttttg aagatttttc cattttagtt tttttttgaa atattatcct actctattct
     1381 gaaattaagg ttttcggatt cccttttttt tatatatcaa actaactttt agtatccttg
     1441 atacgattta taaaaacttc agcaagatca atctcctgaa catgtgcgca atcatttatt
     1501 ataactttta aatctttatg tccaagctct ttaagttttc tgtataacgg taaaagatga
     1561 tcttccacag gacaaatctc atcattgtat ccgcaaataa tgagaatctt tttctttaat
     1621 aattttttgg aatgcctgac aaaatcatat ctgcatatat tatcagtaag atctttgaca
     1681 aatacttctt tttcctgatt gactactccg tttggtaaga aaatattttt agctgtttcc
     1741 tcaaggaatt ctcttatctt tgaatacgga tccataaatt gtcttccaaa gtaaccatgg
     1801 tcacaaagtg agacagcgac tatgttttta attctcgtat catacaaagc tcctacaaaa
     1861 accactgagg aaccaaaact gaatcctccg aaaatcagat tgtttgtgtc aatctgatac
     1921 aaatcggtat ttactttatt atgcaaaaaa tccaaaacgc ttttgagatt gataagagaa
     1981 ttgctgaagt taaattcacc atcattaccc cataaaccct cataattaaa tctgaaaaca
     2041 tttactcctt tttttgaaag ttcaatagac cagggatttg ttccgtcttc cggacctccc
     2101 ggatttccct gcagccatat taaagttggt ttctttttac tcccgctgcc tttataaaaa
     2161 tatgcgcaga gattaatttt attgcttgtg aaatccagct tgtcaatatt tgtcggatct
     2221 gatttccttt tcataattaa atataatttt tagttacctg aagaatactt agaccggctt
     2281 tttgtaaata aacatgttta tatctttcga atcaatttta gaagttgttg gatttccaaa
     2341 aaagtttgaa aaatttaata ttcagaaaaa ttttaaagca ggttaaattt ttttcgttaa
     2401 cggtaaaatc ctaaagttaa aataaatata ataaggtttt aaataatcac cacgacctta
     2461 cccgatttat agtttccaca cttgtattat atgaattgat cattttatta taactctgaa
     2521 taagttcatt aaatttttca ttgaactcat cttcactttt tgatttgggt gcacttccaa
     2581 aaaacacctt tcccgcatta ataaaacttt cggcagatgg agcaaaataa tcttcgtaat
     2641 gatatttcat aaattttact gtctctccga atgaggcttt cataacagtg gatttacttt
     2701 ctctaacggt cttttcaaac tcattgaatt tagtttcagg aactgacagg tcacttttat
     2761 attcaatctt ctcaatgctt tcataaaaac cttctgctga ttcaagtata tctccatata
     2821 cattcagcat agtctgagct gaagcttcat caggatctga tacagaggag gggtcataaa
     2881 taattcttgc aggtttatat tttttcagat ccgctgcgaa cttatcaaat tcagttttat
     2941 atttattgta aacattttcc atgtctttat cataaggaac aatctttgac atatcattta
     3001 catattctct tttgaaataa tattcagcta ctctataaga aactctataa tattcctcta
     3061 ataccggtaa tagatttttc agatcggatt ctaaagtttt ctgcatttcg tcagaagctt
     3121 ttagtttgga aagttctccg ccgtcgagaa gtgatctctg ataatctttg acagtgtttc
     3181 cgagaaattg acattccatt tgcatactca tatttaatat ataagaattt ttattaatct
     3241 gtccaggtgg gggcacttct cttttataat atttataaat attctctccc tgtccctgaa
     3301 tcttgttcat cacttcaata tatttattat aaaatttcag atcttcttca gaagttttat
     3361 ctgaagtgga tactttctct gaaatttttt taccgccgga gatcagcttt tctttaatgt
     3421 ttgaaataaa tgagcagccg gtaagtatta gtaaactgaa aataagtaaa gcaaatgatt
     3481 ttagttttga cataggtttg gatttgattg aacttaattt aataaagcat aaattgaaat
     3541 tcactgaaca aaagtcagga aagtattaag gaattttctg cactatcatt taatacactg
     3601 cgaattcata tgttaagaga ttttttttta ctaacttcaa atcatattcg cgctaaattt
     3661 ttgataccag cattgtttga atttttttgt aagtaaaaat taattaatag tttaatccag
     3721 cacttcatag acatttaccg gaatgctttt attttttaaa attacttctc cgactttccg
     3781 gcaattaaaa gattctttta tttcttcata tgccggttcg cttaataata tttgacccgg
     3841 ttctgcagtt gattgtattc tttgtgctgt gttgactgta tctccgatca cagtataatc
     3901 cagtcttttc agattggctg aaccaatgtt gcctgagatc atttcaccgc agttgagtcc
     3961 gatggatact ttaggattaa aatttacact ttttgataat gcaggtaact tttctatttg
     4021 atttctaaca gcaagactag catctactgc cctgtccaga tgatagtcgc cacgaaaaac
     4081 agccattatt gcatcgccta taaatttatc cacatagcca ccctgaccaa taatctcttt
     4141 tacaattata tcaaaataac tgttgagtaa tttcacgact gtatccgggg gttctatttc
     4201 actgatggta gtaaaactac agatgtcaat aaaaacgaca ctcgcctcta tagtttcgtt
     4261 tgccattaaa gaggtttcaa actcccggct gtccataaaa ttgagtactg tttcatccac
     4321 atacattttc agtatattat tttcctttat tgcttttaaa gtatttttga tttgtaaaac
     4381 ttgatttata gtcttctcca ttgtgactgc aagatcttca aaattaatgg gtttggtgat
     4441 gaaatcaaat gcgccgcggt tcatggctgt gcggatattt tccatatcac cgtatgcaga
     4501 aactataact gatttgagca aaggatttga tacattaagt ttgctgagta aagtcaatcc
     4561 atccatctca ggcatgttga tatcggtaag aacaatatca atatcagtgt gctgtttaag
     4621 tttctccagt gcgtcatttc cgttcacagc aaatacaaat tcatattcat gttcacggat
     4681 cttctgtctg aacttttgtt tgatgagaat ttcaagatca gtttcgtcat cagctactaa
     4741 tatttttgcc attattttat attttaagtt tttcttttaa taatttgaaa tcaactggtt
     4801 tagttaaaaa atcatccgct cccagctcct gagctgtttt aaaattttct ccatcaccgt
     4861 aagctgttat catcattaca accggcggtg gtttgtgata tttctttttg atatgctcaa
     4921 gcagttccag tccgctcatg cccggcatat ttatgtcgga taatatcaga atagcttcat
     4981 gttccaattc atgcagactt aaatattcaa tcgcttcttc accggaaaaa gtaaaagcaa
     5041 attccactat tttatctttg atctcttttc tgaaacgctg ttcaaaaaga gtttgtatat
     5101 ccttttcgtc atctactact aaaattttca ttccatatat ttaatttttt aaatatggta
     5161 aaacaactga aaattctgta ccctcacctg acttcgtctc cactttcagc tcacctccat
     5221 gcgctttgac aatatcatag cttagagaca atcccagtcc cgttccctgt ccagtcggct
     5281 ttgttgtaaa aaacggctga aagattttat ccattacttt ctgcggaatg ccgttgccat
     5341 tatccttcac tct
//
LOCUS       DAORVQ010000217         5345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10528_length_5345_cov_5.332703, whole genome shotgun sequence.
ACCESSION   DAORVQ010000217 DAORVQ010000000
VERSION     DAORVQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10528_length_5345_cov_5.332703"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1595
                     /gene="chrA"
                     /locus_tag="PLX80_12925"
     CDS             276..1595
                     /gene="chrA"
                     /locus_tag="PLX80_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQY21738.1"
                     /translation="MTNKPTYKDALKFWIKLGFISFGGPSGQIAIMHEYLVDKKKWIS
                     ESKFLNALNYCMLLPGPEAQQLAAYIGWLLHGIKGGLTAGILFILPSMFILLGLSITY
                     VSYGEIPWVYSLFSGLKPAVVAIVIIALIKIGSKSLVSPLHYIAAASAFVCINFLNIS
                     FPLIILGSILLALLSSKIFPEMLKANSENSNTYTDKLNEDEYYLNNNSVIKGIEFNSS
                     KFLKLTFTFLILWILPFIIIYTYSNNFEFWKTLYLFFTKTAFVTFGGAYAVLPYVAQF
                     SVENLKWLTDLQMIDGLALGETTPGPLVMVLAFVGFMAGYNHFDSSLLMGSLGLTATV
                     YFTFLPCFYFIFAGAPLIERTQKNNKLKTSLSIVTAAVVGIILNLTIYFGKAVIFPNG
                     ISVQSIDIPSFIWILISLIALYKYKVNIILWIVISAVFGLVYYLLAG"
     gene            complement(2004..3464)
                     /locus_tag="PLX80_12930"
     CDS             complement(2004..3464)
                     /locus_tag="PLX80_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21739.1"
                     /translation="MNQTKLILTLKSFSRDEIKLFEKFIASPFFNNGRNYIPFFTELK
                     KFYPSFESENLNAEFIYEKLYQGKKFNRQVMWNFVSQLEKLALEFLIHNSLKQNRSER
                     FYLISDELLKRNLDKHLLKEIESMEKILSTAKLSIEYYKDKLLLEDSKAEYWYVLQGR
                     QDLSFAGLVGSTEFLILKMIAELSVLVSDLQILKIMYNDGKETNAAIELVKSLNLNNL
                     VTASEINENKYAKVYKFYYDKIMCALDENDETHFFEMKKFFDENYFLFDLNEQSNTII
                     SLANYCAHKMRLGIDKYLRILFELNKFRLEKNIGIHSNGNLNKALFHQILRNALSLGE
                     TDWAENFVVNYTPKLKINHQKTMNALAMGFICYAKKDFTGSLDYLSKVEFIDIRDKLH
                     VRILSAKAYYELNNTETLFYFIDSSNHFIKKNTLIGIETRDSYLKFFNYLKKLLICKD
                     NYNKNKVMELNSDINTDQILRLRHKEWLLEKLSELK"
     gene            3643..3867
                     /locus_tag="PLX80_12935"
     CDS             3643..3867
                     /locus_tag="PLX80_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21740.1"
                     /translation="MKDQKSYSEIKTSRGYRLKISTHVLIEKIQKLINSSKDSVISNS
                     VRLYYERLNQDLKISEVNKKLSKKIKLLKK"
     gene            3881..4912
                     /locus_tag="PLX80_12940"
     CDS             3881..4912
                     /locus_tag="PLX80_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21741.1"
                     /translation="MKTLIFIILLLSCSSYSQTTLTSAINPSAGDVDNYKICDTANVS
                     EGNAGLNQNWNFSGISFIDSTSVTFVNSGSTPYGSQFPGSNISSSNDNVNYNYMTTSE
                     TNLMINGYGGPDGVIINSDAQMFMQFPFTFNSSFTDNFSSDYTLSGTQIFRTGTTTVT
                     GDAWGTIVLPSGTFTNALRVKYIILTKDSSFIGTPFVTLISTNSYVWFIPGRKFPVFE
                     IVYSTISINGIVFGNNKTVNYNQDPVSGISVISSEIPEDFNLSQNYPNPFNPKTVINY
                     ELQVTGNAKLKVFDVLGNEVAEPVNEKQNAGSYSVDFDGSGFASGTYFYSLYIDGRLL
                     ATKRMMLLK"
BASE COUNT         1738 a          872 c          824 g         1911 t
ORIGIN      
        1 tttaaagttc aggctcctcc ccttttttca aaggggaggc tgggaggggt ttgatgatag
       61 cgacgatact tcgccttaaa cttaatgtca ttgctcggct ctcacctttg aaaagcaatg
      121 aagggggaaa agcaataaat tttacattct gaagttcaag ctcctcccct tttttttaaa
      181 ggggaggctg ggaggggttt gctcagtatt tgaataactg aatttatgac aaataaaata
      241 gtatgtatat tatcaaaatt tatttttaaa atttattgac aaataaacct acatacaaag
      301 acgccttaaa attctggatt aaactcggtt tcatcagctt cggaggaccc tctggtcaga
      361 tcgcaattat gcacgaatat cttgtcgata aaaaaaagtg gattagtgaa agtaaatttc
      421 tgaatgcgct gaactactgc atgcttttac ccggacctga agcacagcag cttgcagctt
      481 acatcggctg gcttcttcac ggaatcaaag gcggacttac tgcagggatc ttgttcatac
      541 ttccttcaat gttcatactt ctcggactca gcattactta tgtttcctat ggtgaaattc
      601 cgtgggtcta ttcattattc agcggactta aaccggctgt agtcgccatt gtaataattg
      661 cattgataaa aatcggatcg aaatctcttg tctccccgct tcattatatt gccgctgctt
      721 ctgcttttgt ctgtattaat tttttaaata tatccttccc gctgattatt cttggatcaa
      781 ttttacttgc acttttatct tcaaaaattt ttccggaaat gctgaaagca aattctgaaa
      841 actctaatac ttatacagat aaattaaatg aagatgaata ttatctgaat aataattctg
      901 ttataaaagg aatagaattt aactcatcaa aatttctcaa attaacattt acatttttaa
      961 tcttatggat acttcctttt ataattattt atacctattc aaataatttt gaattctgga
     1021 aaactctcta tctgtttttt actaaaactg cttttgtaac attcggtgga gcgtatgcag
     1081 tgctgcctta tgtggctcag ttcagtgtgg agaatttaaa atggctgacc gatcttcaga
     1141 tgatagacgg acttgcactc ggagagacaa caccgggacc gcttgtgatg gttcttgcat
     1201 ttgtcggatt tatggcgggt tataatcatt ttgacagttc tttattaatg ggatcacttg
     1261 gactcaccgc aactgtttac tttacttttc tgccgtgctt ttattttatt tttgccgggg
     1321 caccattaat agaaaggaca caaaaaaata ataaattaaa aacttcactg agcatagtaa
     1381 ctgcagcagt agtaggtata attctaaatc tgacaatcta ttttgggaaa gctgtgattt
     1441 tcccaaatgg aatttcagtt caaagcattg acattccgtc atttatctgg atcctgatat
     1501 ctctgattgc attgtacaaa tacaaagtta atatcatact gtggattgtg ataagtgctg
     1561 tatttggatt agtgtattat ttattagccg gatgatattt catacaaatc agattcaaac
     1621 tttgcacaac tatggaaatt aatttatgat caaactaaat cgtctgccca aacctgtttc
     1681 aaaaatttta taatgagaag atagttgaat atcacattta ccattttaaa ttcttgagat
     1741 ttaacttttc tttgtgccaa ttttatttgc aagttcttcc tgtgtaagat ttgcagcttt
     1801 tctttcatgt tttagaattt cactaatcac gaaatatttt gcttcagctt cagacttctc
     1861 ttttatttcc taaaagattg agactatact ggtgttcctg aaagtttgac aaatcttgag
     1921 cgaagaataa tttgaaattg aactcaaact atcagctcac caataagctg agaccaatag
     1981 tccgaccggc gaaaagattc ttattatttt aattccgaca atttttctaa aagccattct
     2041 ttatgtctga gtctcaggat ttgatcagta ttaatatcgg aatttaattc cataacttta
     2101 tttttattat aattatcttt gcaaatcaaa agctttttta aataattaaa aaatttcaga
     2161 tatgagtctc ttgtttcaat tcctattaaa gtatttttct taatgaaatg atttgatgaa
     2221 tcgataaaat aaaaaagagt ttcagtgtta ttcagttcat aatatgcttt tgcagaaaga
     2281 attcttacat gtagtttatc gcgaatatct atgaactcta cttttgacag ataatccagt
     2341 gaacctgtaa aatctttttt cgcataacat ataaaaccca tagcaagagc attcatggtt
     2401 ttctgatgat tgattttaag ctttggtgta taatttacaa caaaattttc agcccaatct
     2461 gtctcaccta atgaaagtgc attccttagg atctgatgaa aaagtgcttt attcaaatta
     2521 ccatttgaat gaatcccgat atttttttca agcctgaatt tatttaactc aaacaatatc
     2581 ctcagatatt tatctatacc aagacgcatt ttatgtgcgc agtaatttgc gagagaaatt
     2641 atggtattac tctgttcatt gagatcaaat aaaaaataat tttcgtcaaa aaactttttc
     2701 atttcaaaaa aatgagtctc atcattttca tccagagcgc acataatttt atcataataa
     2761 aatttataca ctttagcata tttattttca tttatttcgg acgcagttac aagattattt
     2821 agattcagac ttttgacaag ttctatagct gcatttgttt cctttccgtc attatacatt
     2881 atttttaaga tctgcagatc tgagaccagt acagaaagtt ccgcgatcat tttaagtatc
     2941 aaaaattcag tgcttcctac cagacctgca aaactcaaat cttgtcttcc ctgcagtaca
     3001 taccagtatt ctgctttgct gtcttccagc aacaatttat ccttataata ttcaatactt
     3061 aattttgcag tacttagtat tttttccata gattcaattt ctttaaggag atgtttgtcc
     3121 aggtttcttt ttaaaagttc atcagagata agatagaatc tttcagatct gttttgtttt
     3181 agagaattat gaatcagaaa ttccagtgca agtttttcca gttgagatac aaaattccac
     3241 ataacctgtc tgttaaattt tttcccctga tataatttct cataaatgaa ttcagcattt
     3301 aagttttcac tttcaaagga aggatagaat tttttaagtt cagtaaaaaa aggaatgtaa
     3361 tttcttccat tattaaaaaa gggagatgct ataaattttt caaacaattt aatttcatcg
     3421 cgggaaaatg attttaatgt aagtattagt ttagtttgat tcaaaccttt ctctttttaa
     3481 atatcaataa ttatacaaaa ttaatcaatt tctgataata agatgcagta ataattaaaa
     3541 atgttgatta gtatcacatt tatctttctt tgattatata tacccttcat gcatattttt
     3601 gtaatgtgca caaagtagtg cactaacata taagataaaa ttatgaagga tcaaaaaagt
     3661 tattcagaaa taaaaacatc aagaggttac agattaaaga tctctacaca tgtgctaata
     3721 gaaaaaatac aaaaattaat taacagcagt aaagattctg ttatcagtaa ttcagtcagg
     3781 ttatattacg aaaggttaaa tcaagatctt aaaatatccg aagtaaataa aaagttatca
     3841 aaaaaaataa aactattaaa aaaataaact caaatcaaaa atgaaaacat taatctttat
     3901 tattttgctt ttaagctgca gctcatactc tcagaccact ttaacaagtg caataaaccc
     3961 gtctgccggg gatgtagata attataagat ctgtgatact gctaatgtat ctgaaggtaa
     4021 tgcagggtta aatcagaact ggaatttttc gggaatttca tttatcgaca gtacttcagt
     4081 tacatttgtg aattcaggat cgacaccata tggatcacag tttccgggtt ctaatatttc
     4141 ttcttcaaat gataatgtaa attataatta catgactact tctgaaacaa atcttatgat
     4201 caatggctat ggcggacctg atggagtaat tataaacagt gatgcacaaa tgtttatgca
     4261 gtttcccttt acatttaaca gttctttcac tgataatttt tcatcagatt atactctaag
     4321 cggtactcag atattcagga ccggaactac aactgtcacc ggagatgcat ggggtacaat
     4381 tgtacttccg tcaggtactt ttacaaatgc attgagggtc aaatatataa ttttaacaaa
     4441 agattcatct tttattggaa caccgtttgt cacattaatt tcaacgaatt cttatgtatg
     4501 gtttatcccc ggaagaaaat ttcctgtttt tgaaatagtt tattctacaa tttccataaa
     4561 cggaatagtt ttcggaaaca ataaaacagt aaattataat caggatcctg tatccggtat
     4621 ttcagttatt tcttcagaga ttcccgaaga tttcaatcta tcacaaaatt atcctaatcc
     4681 atttaatcca aaaacagtta tcaattacga actacaggtt actggaaatg caaagctaaa
     4741 agtatttgat gtattgggga atgaagttgc agaacctgtt aatgagaaac agaatgccgg
     4801 aagttattcc gttgattttg acggaagcgg ttttgcgagc ggcacatatt tttattcatt
     4861 atatatagac gggagattat tagctacgaa gcggatgatg ttgctgaagt gatttttcaa
     4921 ccccaccctg ccctttcgtc attatttttg cttttattaa attgtaaaaa tttcttttgt
     4981 ttgtactatg ttggaggatt tacctgacac aataagtaaa tcacttaatg cttcgagtga
     5041 tttacatgac gatatatgtt aatcacttat tgcttcgggt gatttacatg acgctatatg
     5101 ttaatcactt aatgcttaag gtgatttaca tgacgctata tgttaatcac ttaatgctta
     5161 cggtgattta cttgacaata tatgtaaatt acttattgcg tccggtgatt ttccttacgc
     5221 tgtaagtaaa tcacatattg cttccccaaa attaaataat gcatgtttct tttttcatag
     5281 tccgttaaaa aaaattataa acgcgtttat taaattaaat atcgcgttat tgaaaaataa
     5341 aattg
//
LOCUS       DAORVQ010000218         5337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10554_length_5337_cov_5.179288, whole genome shotgun sequence.
ACCESSION   DAORVQ010000218 DAORVQ010000000
VERSION     DAORVQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_5337_cov_5.179288"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..785
                     /locus_tag="PLX80_12945"
     CDS             75..785
                     /locus_tag="PLX80_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY21742.1"
                     /translation="MELFSGFIIGLLGSFHCIGMCGPIVLSLPGGLFSKWSFILNRLL
                     YNSGRIITYMLLGAVFGLLGNRLNLYGLQQMISIIIGALILAAVLAGLYMKKFNSLTL
                     LQMKMVSGLKKILKINFDRRSKSTFLITGIINGILPCGFVYIGLSGAMVTGEFTRGAL
                     YMILFGLGTIPLMLGLSLFGNFVSLKFRNLLKKLTPVFAIILAILFILRGLDLGIPYV
                     SPAHKIDSQIIDPQVQCN"
     gene            937..1644
                     /locus_tag="PLX80_12950"
     CDS             937..1644
                     /locus_tag="PLX80_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQY21743.1"
                     /translation="MRKFHSPSCESCSSRFKSVFCDLPDEEISQISDSKHCNFYLKGQ
                     SLFNEGNHPDGLFCINKGRVKLSQTGFEGKEQIIRLAKDGDVMGYRSLISGETYSASA
                     TVMEDSRICIIPKSVFFDLLRKNINLTGKVMKLLADDLKFAQNKITNLAQKPVIERLA
                     EALIMLKEYYGMKEDESYLNITITREEIANIVGTATETTIRLLSELRKEGILELEGKK
                     IKILKNDALLKLANLYD"
     gene            1663..2073
                     /locus_tag="PLX80_12955"
     CDS             1663..2073
                     /locus_tag="PLX80_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQY21744.1"
                     /translation="MFEKFSNSFLKDFDINYIIERFENKSFRTYLKDDNIIYQGNSPD
                     SVYFILSGKINIGNIMPDGNFDSHYELIGGDILGIENALRNENYSHSAIAESETNVMI
                     ISKDEMISFNGINDDFNIWLLKYLSNRISKLESN"
     gene            complement(2108..3106)
                     /locus_tag="PLX80_12960"
     CDS             complement(2108..3106)
                     /locus_tag="PLX80_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HQY21745.1"
                     /translation="MKKNIIVQEKFYDVLIIGAGPIGLACGIECEKNSLPYIIIEKGC
                     LVNSIFNYPTNMTFFSTSEKIELSGVPFVSHGVKPNRREALEYYRRVKESFGLNVNIY
                     EEVLSITKSANSFKVRSDKNIYNSRFIIIATGYYDNANLLNIPGEDLSKVRHYFDEPH
                     PYSGQNVAVIGAGNSAVDVALELFRVGANVTMIIREDKIKPSIKYWVKPDIENRIKEG
                     SIKTFFESEIIKIESKELTLQQKNKKINIKNDFVFSMTGYHPDFSFLKKAGIKIGYDN
                     TPEFNLKTFESNIKGLYLAGVVCGGINTDRYFIENSIKHSEIIINEIIKEISVRAT"
     gene            complement(3111..4535)
                     /gene="pyk"
                     /locus_tag="PLX80_12965"
     CDS             complement(3111..4535)
                     /gene="pyk"
                     /locus_tag="PLX80_12965"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQY21746.1"
                     /translation="MIYKKTKIICTIGPAVGDVEKICMLIDAGMDAARLNFSHGTHEI
                     HKQYINNIRKASKIKGKIIAIIQDLSGPKIRVGKLENGSIVLEEGKMIFITSKNITGN
                     IKMISTNYNSLLKDLRKNQFILLDDGKLKLRVVSERPFQRKIECEIISGGILKENKGI
                     NLPNTKLSIPFLTEKDREDLKFGLKNKVDFVAVSFVRTEYDIKHVKELLKFHNRIVPI
                     IAKIERPEAVENIDSIIRESDMIMVARGDMGVEISTEEVPIIQKRIIKKCNDAIKPVI
                     TATQMLESMIENPGPTRAEASDIANAILDGTDCVMLSAETSTGKYPIQAVANMKKIIL
                     KTETIKKSSYFKEMFVEDSAENTLHTICNSATEIATRLNARAIITISHTGKSPLLLSS
                     HRPNAQIISATYIESIIKKCKLIWGVESIFIEKADNMKDTIDLITKKILLNKILKKKD
                     RIIIITPMPFSYTESANMILVTQL"
     gene            4613..>5337
                     /locus_tag="PLX80_12970"
     CDS             4613..>5337
                     /locus_tag="PLX80_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY21747.1"
                     /translation="MIDLKEKTVLITGATSGIGKAAAFKFAENKSNLILVARRKERLD
                     EISEILSTEFKIKVHSIEADVRNYESVKAFIESLPDDFKKIDILINNAGLARGFSNIS
                     DGSVEDWDEMIDTNVKGLLYVSKCIVPLMIKNNSGHIINIGSVAGREVYPNGNVYCAT
                     KHAVDAITKSLRMELVSSPIKVTTIDPGLVETEFSLVRYRGDSKKAGVVYSGMKPLTS
                     EDVAEAIIFAVTRNDNFVVAEMVL"
BASE COUNT         1715 a          849 c          869 g         1904 t
ORIGIN      
        1 attaaatttg aattaaatgg aaatggtgaa aattattttg ttgaaaaaaa tatttatcta
       61 aattaactga tataatggaa ttattcagcg gcttcattat cggattactt ggcagcttcc
      121 actgtattgg aatgtgcgga cctattgtgc taagtttacc cggaggttta ttttccaaat
      181 ggtctttcat tctcaacaga cttttatata attccggtag aataattaca tatatgttac
      241 ttggtgcagt ttttggtttg cttggaaacc ggttgaacct ttatggactt cagcaaatga
      301 tttcaataat tattggagct ctaatcttag cggctgtctt agcaggatta tacatgaaaa
      361 aatttaattc attaactctt ttgcagatga aaatggtatc cggattaaaa aagattttga
      421 agatcaattt tgatagaaga tcaaaatcta catttttaat tacgggaatt attaacggca
      481 tactaccatg cgggtttgta tatattggac taagcggtgc tatggtgacc ggtgaattca
      541 cacgcggagc attatatatg atattgtttg gacttggtac aattcctctt atgctgggtt
      601 tgtctctgtt tggaaatttt gtaagtttga aattcagaaa cttgttaaaa aagctgacac
      661 cggtttttgc aattatactt gctatactat ttatattacg gggtctggat cttggaattc
      721 cttatgtaag tcctgctcac aagattgatt cccaaataat tgatccacag gtacaatgta
      781 attaatacat acctggctat acagttcttt ataatatcga aattttcttt cgcagtttta
      841 aatagatcac tggacattaa tatttattgt gttctaaatt gtaattaaca tgtttaaaaa
      901 ttaaatttag tggtcaaaaa ttatattttt aatttaatgc gaaaattcca ttcaccatct
      961 tgtgaatcat gcagctcaag attcaaatct gtattttgtg atctgcctga tgaagaaatt
     1021 tctcaaatat ctgacagtaa acactgtaat ttctatttaa aaggtcagtc attgtttaat
     1081 gaaggaaatc atcctgatgg tctattttgt ataaataagg gcagagtgaa actgtctcaa
     1141 acaggttttg aaggaaaaga gcagatcata agacttgcaa aagacgggga tgtaatgggt
     1201 tacaggtctc tgataagcgg tgaaacttat tctgcatcgg ctactgtgat ggaagattca
     1261 aggatttgca ttattccaaa aagtgtattt tttgatcttc tcagaaaaaa tattaattta
     1321 acaggtaaag taatgaaact tcttgcagat gatctaaaat ttgctcagaa taaaattaca
     1381 aaccttgctc aaaaaccggt aattgaaaga cttgctgaag ctctcataat gttaaaagaa
     1441 tattatggaa tgaaagaaga tgaatcttat ctgaatatta ctatcacaag ggaagagatc
     1501 gcaaatattg tcgggacagc aacagaaact accatccgtc ttttgtctga actaagaaaa
     1561 gaagggatac tcgaattaga gggaaagaaa attaagatat taaaaaatga tgctttgctg
     1621 aaactggcaa atctttacga ttaataattt taattaacac atatgtttga gaaattctca
     1681 aattcctttc taaaggattt tgacatcaat tatattattg agagatttga aaataaatct
     1741 tttcgtacat atctgaaaga tgataatatt atttatcagg gtaattctcc tgacagtgta
     1801 tactttattt tgtccggtaa aataaatatt ggaaatatca tgcctgatgg taattttgat
     1861 tcacattacg aactcatcgg tggagatatt cttggaattg aaaatgcttt gagaaatgaa
     1921 aattattcac attctgcaat tgctgaatct gaaacaaatg taatgatcat ttcaaaagat
     1981 gaaatgatct ctttcaatgg tatcaatgat gatttcaata tttggttatt aaaatatctg
     2041 agtaacagga taagtaaact tgagtcaaat taagtctttc ttcagaagag taatgtcaca
     2101 aaaaaaatta tgttgctctt acagaaattt ctttgatgat ttcattaata attatttcag
     2161 aatgtttaat agaattttca ataaaatacc tgtcagtatt tatcccaccg caaacaactc
     2221 ctgccagata gagtccttta atatttgatt cgaaagtttt taaattgaat tcaggtgtgt
     2281 tatcatatcc tatttttatc ccggcttttt ttaaaaatga aaaatcagga tgataacctg
     2341 tcatagaaaa aacaaaatca ttcttaatat ttattttttt attcttttgc tgaagtgtaa
     2401 gctctttcga ttctatctta ataatttcag attcaaagaa agttttaata gaaccttctt
     2461 ttatcctgtt ttctatatca ggcttaaccc agtattttat agatggtttt attttatctt
     2521 ccctgattat cattgttaca tttgctccaa ctctgaataa ttccaaagct acatctactg
     2581 ctgaattccc ggcacctatt actgcaacat tctgtccgct gtaaggatga ggttcatcaa
     2641 agtaatgccg cactttgctt aaatcctctc cgggtatgtt aagtaaattg gcattgtcat
     2701 aataacctgt agcaattatt ataaatctgg aattgtaaat atttttatca gatctgactt
     2761 taaaactgtt tgctgatttt gttattgata atacttcctc ataaatattt acattcaggc
     2821 cgaaagattc ttttactctt ctataatatt ccaaggcttc ccttctgttt ggctttactc
     2881 cgtgagacac aaaaggtact ccgcttaatt ctattttttc agaagtagaa aagaatgtca
     2941 tgtttgtcgg gtaattgaat atagaattta caagacatcc tttttcaata atgatatatg
     3001 gtaaagaatt tttttcacat tctattccac atgcaagtcc tattggaccg gcaccaatta
     3061 tcagaacatc ataaaatttt tcttgtacga taatattttt tttcatgaat ttataactga
     3121 gtaactaata tcatattcgc agattcagta tatgaaaaag gcataggtgt tatgattatg
     3181 atcctgtctt tcttcttaag aatcttattc aaaagaattt tctttgtgat cagatctata
     3241 gtgtctttca tattatctgc cttttctata aaaatagact ctacacccca gatcagttta
     3301 catttcttta ttatgctttc aatatatgtt gcagaaatta tctgggcatt tggtctgtga
     3361 ctggaaagca gtaaaggaga tttacctgta tgagaaatag taataattgc cctggcattt
     3421 aatcttgttg ctatctctgt agctgaattg caaattgtat gaagagtatt ttctgctgaa
     3481 tcctcaacaa acatttcttt aaagtatgaa gacttcttga tcgtttccgt ttttaaaatg
     3541 atttttttca tatttgcgac agcctgaata ggatacttac cggttgatgt ctctgccgaa
     3601 agcattacgc aatcagttcc gtcaagaatt gcatttgcta tatcggaagc ttcagccctt
     3661 gtaggtccgg gattttctat catggattcc agcatctgag ttgcagttat tacaggtttt
     3721 attgcatcat tacatttttt tataattctc ttttgaatta taggaacttc ctcagtagag
     3781 atctctacac ccatatctcc gcgtgccacc attatcatat ctgattccct aataattgag
     3841 tcaatgtttt ctacagcttc aggtctttct atctttgcaa ttatcggaac tatacgatta
     3901 tgaaatttca ataattcttt cacatgctta atgtcatatt ccgtcctcac aaaactaact
     3961 gctacaaaat caactttgtt ctttaatcca aacttcagat cttctctgtc cttttctgtc
     4021 agaaaaggaa tgctgagttt tgtgtttgga agattaatac ctttattctc ttttaaaata
     4081 cctcctgaga ttatttcaca ttctatcttt ctctgaaaag gtctttcaga aactacacga
     4141 agtttaagtt ttccatcatc aagcagaatg aattgatttt tcctaagatc ctttaaaagc
     4201 gagttgtaat ttgtcgatat cattttaatg ttgccagtga tatttttgga agtaataaag
     4261 atcatttttc cttcttccag tactatgctt ccgttctcca gtttacccac tctgatcttt
     4321 ggacctgaaa gatcctgaat tatcgcaatt atctttcctt ttatcttgga agcttttctg
     4381 atattattta tatattgttt gtgaatctca tgcgtcccgt gagaaaaatt cagtctcgct
     4441 gcatccatac cggcatctat cagcatacat attttttcaa catccccgac agccggtcct
     4501 attgtgcaaa taatttttgt tttcttatag atcatacttt acttattttg ttaattcaaa
     4561 aatacgagtt tcaaaatatt cattcataaa ctttatttaa agctaaatac caatgattga
     4621 ccttaaagaa aaaacagtcc tgattacagg agctacaagc ggaattggaa aagcagcagc
     4681 atttaaattt gcagaaaata aatcaaattt aatactcgtt gccaggagaa aagaaagact
     4741 cgatgaaata tctgaaatac tttctacaga gttcaaaata aaagttcatt caattgaagc
     4801 tgatgtgagg aattatgaat ctgttaaagc atttattgaa tcacttcctg atgattttaa
     4861 gaaaattgat atactgatta acaatgccgg acttgcaaga gggttcagca atatctctga
     4921 cggatcagtt gaagactggg atgaaatgat agatacaaat gtaaaaggac ttctttatgt
     4981 ctcgaaatgt attgtaccat taatgataaa aaataactcg ggacatataa taaatattgg
     5041 atcggtcgca ggcagggaag tctatccaaa cggaaatgtt tattgtgcga caaaacatgc
     5101 tgtcgatgca attacaaaaa gcctaagaat ggagctggtc agttctccaa taaaagttac
     5161 taccattgat ccgggtcttg tggaaactga attcagcttg gtccgataca gaggtgattc
     5221 taaaaaagca ggtgttgtct attcaggaat gaaacctctt acctcagaag atgtagccga
     5281 agcaattatt tttgctgtta cgagaaatga taattttgta gtcgctgaaa tggtact
//
LOCUS       DAORVQ010000219         5275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10740_length_5275_cov_5.585441, whole genome shotgun sequence.
ACCESSION   DAORVQ010000219 DAORVQ010000000
VERSION     DAORVQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_5275_cov_5.585441"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..3253)
                     /locus_tag="PLX80_12975"
     CDS             complement(389..3253)
                     /locus_tag="PLX80_12975"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21748.1"
                     /translation="MNNLPSGNLTFLFSNADDITKLALEFPDKISAAQKKLNEIIQKA
                     VSINNGHIFKKAGDDYCCSFEKAADAAKAAIQIQRDLNSEDPKEILLSSRIGIHSGTA
                     EWKKDDYKGYVTLARTNRIMSAGSPGQVLISNVTYELVRFDLPESVTFRDLGERILKD
                     LQEPLRLFQLNARGLISEFTPLKTLDARPNNLPVGLTNFIGREKEMKELKIMLNSSRL
                     ITLLGTGGTGKSRLAIQIGAEKIDEYENGVRLIELASLRDPELLYNTIAAVLNLKEDG
                     SDDSGDKIINYLKEKKILLIFDNCEHMIIQCAEICKNILNKCPGVNILTTSREVLGVE
                     GEMIYQVPTLSMPGPEDGQTAESISHFESIQLFTERAVALKNDFVLSDGNASDIAKIC
                     YHLDGIPLAIELAAARVKILKPEKILENISNRFRFLTGGSRISLPRQQTLKAMIDWSF
                     DLLSENEKILFRNLSVFTGGWSLEAAEEVCVTEGIDIYEVLDLISNLSNKSLIISKEI
                     NGNIRYNMLETIYEYSLEKLNSDKEIYKRHFEYFLKLSDSDEKNGNDEQENEWGKDME
                     TESGNLRAAVKWSLENSYEKSAKLIINAAEYWEGKGNYSFALEAIKKIIQPDADPEDD
                     EIAAVYINAAFLACQLDDNKNSEKYILKALEYFRRTENKTKTADALSIYGMLSFVKGE
                     PENAKKHYEESLSLLKDTDNLQLLSNVKNNLATLYSATGEMETSLKLLDEVVEIQRNF
                     NKKNLLAKTLVSKGGAYYQNGDLEEARECYMESLSLLEYFDDHYSTATVLYNLGNLNF
                     ALMNYPVALDYYQKAMKVAAEYGLKGVLDRSKIKLGEIFLMQNENAKAKKIFIECLLS
                     FSKDSERLKLNLCIYGLGKYYFYEKEYESAVKLFYITERISEQIGFKFSKSRKDENNS
                     IMEEIKSNLSSDKLDEIHKEVMTYDLDKAGVISIDLAKEE"
     gene            complement(3847..4608)
                     /locus_tag="PLX80_12980"
     CDS             complement(3847..4608)
                     /locus_tag="PLX80_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQY21749.1"
                     /translation="MTDEKLAVLIDADNVPYANVKEMMEEISRNGTPTIKRIYADWTK
                     PTVAGWKNVLLENAITPVQQYSYSTGKNSSDSALIIDAMDILYSEKVDGFCIVSSDSD
                     FTRLATRLREAGMTVIGFGEKKTPKPFISACNKFIYLEILKTPAAEIKTISKKSPKPR
                     SKSKSISRTEPLDRVDAETIRIITDSVNDLADESGWTFLGSLGNLILKKKPDFDPRNY
                     GFTKLLSLIKYSNKFEIDERDTGQKNVRHIYLKLK"
     gene            complement(4649..4777)
                     /locus_tag="PLX80_12985"
     CDS             complement(4649..4777)
                     /locus_tag="PLX80_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21750.1"
                     /translation="MKFGITKKFSFEISPNYSSGFYQTDNMLTGLEYSAGFGYVPK"
BASE COUNT         1573 a          993 c          824 g         1885 t
ORIGIN      
        1 atctacctgt ttcagtctgt aattatacat gccggagtta agatttctgt ccgtgacact
       61 aacggatacg tcatacattt tatttttgct ccctcccaat tccccgattc ggtatgctca
      121 tgaaattgaa actccatttt aagatttccg gcatgacaaa tatatttctc tcctctcctt
      181 gtttatgaga gtttctctaa acatctgttc ttaccaatat gacaccaaga cacaaaggaa
      241 aaaagttaaa aaatattaat ctttgtgact tagtgtcttt gaggtgaaat tatttaactg
      301 ttattagaca gactctttta aggaggcgcc ggggaggttg cggaaagtct aagctttcag
      361 tcacttcgta tatttatttc acaatcattt attcttcttt agccaaatcg atacttatga
      421 caccggcttt atcaagatca taagtcatta cctctttatg aatttcatca agcttatctg
      481 aagacagatt gctttttatt tcttccatga tggaattatt ttcatctttt ctggactttg
      541 aaaatttaaa accgatctgc tcactgatcc tttcagttat ataaaaaagt tttactgcgg
      601 attcatattc tttctcataa aaataatatt tccccagacc ataaatgcat agatttaatt
      661 tcagtctttc agaatcctta ctgaaactca ataaacattc tataaatatt ttctttgcct
      721 ttgcattttc attttgcatg agaaatattt ctcccagctt aatttttgaa cggtccagaa
      781 cacccttcag cccgtattcc gcagcgacct tcattgcttt ttgataataa tccagagcta
      841 ccggataatt cattaatgca aaattaagat tgccaagatt atataataca gtagctgtag
      901 aataatgatc atcgaaatat tcaagtaatg aaaggctttc catataacac tcacgggctt
      961 cttcaaggtc tccattctga taataagctc cgcctttgct tacaagtgtt tttgcaagaa
     1021 gatttttttt attgaaattt ctctggatct ctaccacttc atcaagtaat ttaagtgaag
     1081 tctccatttc ccctgtagcg gaatataatg tagcaagatt atttttaaca ttagataaaa
     1141 gttgtaaatt atcagtgtct ttcagcaaag ataaactctc ctcataatgc tttttagcat
     1201 tttcaggttc gcctttcaca aaagacagca ttccataaat gcttaaagca tcagcagttt
     1261 tagttttgtt ttcagtcctt ctgaaatatt ccagtgcttt caaaatatac ttttcactgt
     1321 ttttgttgtc atcaagctga catgccagaa atgccgcatt aatataaaca gcagcaattt
     1381 catcatcttc aggatcagca tctggttgga ttattttctt aatggcttcg agagcaaatg
     1441 agtaattccc ttttccttcc cagtattcgg cagcatttat tattaactta gcagatttct
     1501 cataggaatt ttcaagtgac catttgacag cagcccgaag attgcctgat tcggtctcca
     1561 tatcttttcc ccactcattt tcctgttcat catttccatt cttttcatct gaatcagaaa
     1621 gtttaagaaa atattcaaaa tgtctcttat aaatttcttt atcagaattt agtttttcta
     1681 aactgtactc gtatattgtt tccagcatat tgtatctaat atttccgttt atctcttttg
     1741 aaattatcag agatttattg gaaagattgg agatcagatc cagtacttca taaatatcaa
     1801 tcccttcggt aacgcagacc tcttcggcgg cttccagact ccatccaccg gtaaatacag
     1861 acaggtttct aaaaagtatt ttttcatttt cagaaagcag atcaaaactc cagtctatca
     1921 ttgcctttaa agtctgctgt cttggcaaag aaatcctgct gcctcccgta agaaaacgga
     1981 aacggttgct gatattttcc agtatttttt caggttttaa aattttaacc cttgcagcag
     2041 cgagttcgat tgcaagcggg atcccgtcca gatgataaca gatctttgca atgtctgagg
     2101 cattgccgtc gctgagtaca aaatcatttt tcaaagctac tgccctttcg gtaaataact
     2161 gtatagattc aaaatgactt atggattcag cagtttgacc atcttccggt ccgggcattg
     2221 aaagagtcgg cacctgatat atcatttctc cctctactcc aagcacttca cggctggttg
     2281 tcaaaatatt tacacccgga catttgttaa ggatgttttt acagatctct gcacattgaa
     2341 ttatcatatg ttcacagtta tcaaatatca gcaggatctt tttctctttc agataattaa
     2401 ttattttatc cccggaatca tccgaaccgt cctccttcag atttaaaact gctgcaatag
     2461 tattatataa aagctccgga tctctcagag atgcaagctc aattaatcga acgccgtttt
     2521 catattcatc aattttttca gctccgatct gtattgccag tcttgacttt cctgtcccac
     2581 ctgtcccgag taaagtgatc agccgcgaag aattcagcat gatcttaagt tccttcattt
     2641 ctttttccct gccaatgaaa tttgtcaatc caaccggtag attatttggt ctggcatcca
     2701 gtgtttttaa tggagtaaat tctgatatca gacctcttgc atttaactga aataatctca
     2761 aaggttcctg aagatccttt aatattcttt ctccaagatc cctgaaagtg acagattcgg
     2821 ggagatcaaa tctgacaagt tcatatgtaa catttgaaat gagtacctga cccggactgc
     2881 cggctgacat gatcctgttt gtgcgtgcaa gtgtgacata acctttataa tcatctttct
     2941 tccattcagc tgtaccgctg tgaattccga tccttgaact gagcagtatc tccttcgggt
     3001 cttcagaatt aagatcacgc tggatctgaa tggcggcttt ggctgcatct gcagcttttt
     3061 caaatgagca gcagtaatcg tcacctgctt ttttaaagat atggccatta ttgatactta
     3121 cagctttctg aataatttca ttaagttttt tttgtgctgc agatatctta tccggaaatt
     3181 caagcgcaag ttttgtaatg tcatctgcat tagaaaaaag aaatgtcaaa ttgcctgaag
     3241 gcagattgtt catttaaaat aaatttttaa taatgtgaaa aataagttcg cataggggat
     3301 catatcaatt tctcagaaag tttatttaat ttacaaaata catttataaa atataatatc
     3361 tataatgtat cctctatgtt atgtgaaact caggagtggg gcgcggggca ttgtaataaa
     3421 gttaagggat tttttaatta taatttgatt aatataaatt tataatttaa aagttcagac
     3481 tcctcacttt tttttaaaat ggtgagactg ggaaaggttt gctgaaagcg atgatcttcg
     3541 ccatttacta aatattattg ggatattgca gaaagacttt tagttaagtc tattttttat
     3601 catagcagtt tcaaaattcg gatcaattcg tcagatgacc ggatgcctga cagtagtagt
     3661 tatccgaaaa cgaagctctc acttttcacc caaaaggaaa aatgaattaa aataatttga
     3721 ttcaaatgtt ttttgctttt agtcaaaatg atttagtgtc taactatcta attaactatt
     3781 tatctgatta tattattatc ttattatcat attatccagt gcctgtgtgg taaatctaat
     3841 tgttaattac ttcaacttca gataaatatg cctgacattc ttttgtccgg tatctctttc
     3901 gtctatctca aacttgtttg aatattttat tagtgaaagc agtttagtga atccgtaatt
     3961 tctcggatcg aaatcaggtt tcttctttaa gattaaattt cccaaactcc ctaaaaatgt
     4021 ccatccgctt tcatctgcca gatcattgac actgtccgtg atgatcctaa ttgtttcagc
     4081 atctactctg tcaagcggct ctgtccttga tatagattta gatttggatc ttggtttagg
     4141 agattttttg gaaatggttt taatttcagc tgccggagtt tttaatattt ccagataaat
     4201 gaatttatta cacgcagata taaatggttt aggagttttc ttttctccaa atccaattac
     4261 ggtcatgccc gcttctctga gtctagtagc taatcttgtg aaatcactat cgctggaaac
     4321 gatacaaaag ccgtctactt tttcagaata taagatatcc attgcatcaa ttatcagagc
     4381 gctgtcactt gaattttttc ctgtggaata actgtactgc tgaacaggtg ttatcgcatt
     4441 ctcaagtaag acatttttcc atccggctac agtcggcttt gtccagtctg cataaattcg
     4501 ttttattgta ggtgtaccgt tcctggaaat ttcctccatc atttctttta catttgcata
     4561 aggcacattg tctgcatcta ttaatactgc taatttttcg tctgtcattt tattttattt
     4621 tatatgataa tgaatataga gttaattatt acttcggtac ataaccaaat cccgcactgt
     4681 attctaatcc ggtcagcatg ttatctgtct gataaaaacc ggagctgtaa tttgggctga
     4741 tctcaaagct gaatttttta gtgattccga atttcatcaa acccctccca tcctccactt
     4801 tgaaaaaatg gggaggagcc tgaattttta aattaaaaat ttacattaat ataattataa
     4861 aataaaaaat cacttagctt aatggtattt cccctttgtc tccctttttt taagagggaa
     4921 ttacttgtaa tttattctga aatcagctaa tagaaattta gacttcattt ataaaaaaat
     4981 tagtgaaaat tttcttacca ttttgagatt aatttagatt gtattaaatt aactaccaat
     5041 atttaaaacg aaaattgaag tatttattct ttgaatacaa aaatgatttt aatattgatg
     5101 taggatcaca aataaatata ttacataaaa aatgaaataa atacttttct ttaatttcca
     5161 gaaacagaga tccagttttt cagatcgatc aaaggctgct gtgagattac aataatagaa
     5221 ctgccgggac ggtcaagtga agctatgata cagattacca atgaaaaaga taaaa
//
LOCUS       DAORVQ010000220         5262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10792_length_5262_cov_5.495487, whole genome shotgun sequence.
ACCESSION   DAORVQ010000220 DAORVQ010000000
VERSION     DAORVQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10792_length_5262_cov_5.495487"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1982)
                     /gene="mutL"
                     /locus_tag="PLX80_12990"
     CDS             complement(105..1982)
                     /gene="mutL"
                     /locus_tag="PLX80_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQY21751.1"
                     /translation="MTENKIKILPQTLSNKIAAGEVVNRPESVVKELIENSLDSGAKN
                     ITLIIKDAGKSLIQVTDDGSGMSEEDALICFQRHSTSKIASIEDLENILTLGFRGEAL
                     ASIASISHAELITRTADSEVGTLLRIEGGEVKENSKSGNETGTSISVRNLFYNTPGRR
                     NFLKSDQTEFRHIYESFVRLALSHPETAFTFFNNDNEVFRLKGTDLKTRISELFGDKI
                     PGYLLDVENGNEIIKVRGFISKPNFTKKSKQDQYFYLNGRFFVSRSLNYAVYSGFDHL
                     IEKGDYPSFFLFIDLHPAKVDVNVHPSKMEVKFEDEGAVFGFIRNSVKEALRKADIVF
                     DIGFDNTISFPDKAESFHYRNDKRDDIKSDRGFTGGGSGSHGSGSQNFFTGGDDRNKT
                     ANIHSIFEASRERTDDSISQIPDTPDTKNDDQRRIFEHSKKSDSANFNVWQYQYKYIM
                     CQTETGLMIIDQHAAHERILYERASMWLDSQSTFSQQLLIPIKFQLTKIDYHIAESLK
                     DEFRNLGFNLNLIKDETVEITGLPSDVKIGDEAKIFQDLIDQFKEYELKLNLDKRDNL
                     AKSFACRSAIKSGDKLTFQEMTSLIDDLFAAKMPYVCPHGRPTLIRITTDELDKRFSR
                     S"
     gene            complement(2245..3189)
                     /gene="ftsY"
                     /locus_tag="PLX80_12995"
     CDS             complement(2245..3189)
                     /gene="ftsY"
                     /locus_tag="PLX80_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQY21752.1"
                     /translation="MAFLDKLGFGKLKEGLNKTKDNLVGKVNRLVNARGVIDDEFLAG
                     LEEIFISSDLGLDTAEQLIERIKDRALTDKYIDQKELNELINSEIRKVFTETTSEFKT
                     FDFDLKQKPYVIIVVGVNGVGKTTSIGKLANNFKKAGKSVLIGSADTFRAAANDQLDI
                     WAKRADVEIIQSKSTSDPASVAFDTVNSAKSKNTDVVIIDTAGRLHNKSHLMEELKKI
                     KRVIQKVIPDAPHETFIVIDATTGQNGLNQAMEFSKALEGLTGIILTKLDGTAKGGIV
                     LKINKEMKLPVRFIGVGEQIDDLQVFDSKSFTEALFEK"
     gene            complement(3283..4428)
                     /locus_tag="PLX80_13000"
     CDS             complement(3283..4428)
                     /locus_tag="PLX80_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY21753.1"
                     /translation="MDFNLSEQELAVQKLAREFAQNEIAPHVMKYDESQEFPMEIAKK
                     LGDIGFLGIIFPEEYGGAGFSTLEYAIIVEEISKVDPSMGLTIAAHNGLCTNHIYSFA
                     NEELKKKYLPDLTTGKKLGAWGLTENVSGSDAAGMATTAERKNGHYIINGSKLFITQG
                     SVGETFVVTAVTDKEKKKNGISAFILEKGMKGFTTGKKENKLGMRSSDTAELIFENVE
                     VPAENLIGEEGQGFKQCMKILDGGRIAIAALSLGIAQGALDASLKYSKERKQFGKSLS
                     HFQGIQFKLSDMATQADAARLMVYKAAWMKDNGMEINLSAAMAKFYASEIATTATNEA
                     IQIHGGYGFMKEFPVEKLYRDVKLMTIGEGTSEVQKMVIARNLLNMY"
     gene            4595..5143
                     /locus_tag="PLX80_13005"
     CDS             4595..5143
                     /locus_tag="PLX80_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21754.1"
                     /translation="MKLNIIKNFAAVMLIFSFTILFISCSKETPQTYFNVSVLNSNIL
                     SGFAGTGFSRQLSSSSEKLKENGTETEPMKRSEVINDKIGQIEEYYNNVSSLNKTDDT
                     KEMIDASLNLYNFVLPVCKTEYRKLAELYDNNASQSDIDKLTLEIQDKYLQKYTDLYS
                     QLIKTGKVYAEKNSIKVNWADE"
BASE COUNT         1538 a         1055 c          828 g         1841 t
ORIGIN      
        1 ctttgagcct ttgagccttt gagcctttga gcctttgagc ctttgagcct ttgaggcaat
       61 ggaataccaa tataaaacaa ataaataaaa cttaaactga ttttttatga cctgctgaat
      121 cttttatcaa gttcatcagt cgtgatgcgg atcagcgtag gtcttccgtg cgggcataca
      181 tacggcatct ttgctgcaaa taagtcatct atgagtgaag tcatttcctg aaacgtaagt
      241 ttgtctccgg attttattgc gctgcggcag gcaaaggatt ttgctaggtt gtctcttttg
      301 tcaaggttca gtttcagttc atactccttg aactgatcta tcaagtcctg aaatattttt
      361 gcctcatcac caatctttac atctgacggc agtccggtga tctcaactgt ttcatccttt
      421 ataagattaa gattaaatcc cagattccga aattcatctt tcagagattc agcaatatga
      481 taatctattt ttgtgagctg gaattttatg ggaattagaa gctgctgcga aaatgtcgat
      541 tgagaatcca gccacatcga agctctttca taaagtatcc tttcatgtgc agcatgctga
      601 tctattatca tcagaccggt ttcagtctga cacattatat atttgtactg atactgccag
      661 acattgaaat ttgcagaatc tgatttttta ctgtgttcaa atattcgtct ctgatcatca
      721 ttttttgtat caggcgtatc aggtatttgc gaaatgctgt catccgttct ttctctcgat
      781 gcttcaaata tagaatgaat gtttgcagtt ttatttctgt catctccgcc tgtaaaaaaa
      841 ttctgactac cgctgccatg actgcctgat ccgcctcctg taaatcccct gtcacttttt
      901 atatcatccc gtttgtcatt tctgtaatga aaactttcag ctttatcagg aaatgagatc
      961 gtattatcaa aaccaatatc aaatactatg tcggcttttc tcaaagcttc ctttacggaa
     1021 tttctgatga atccaaatac tgcaccttca tcttcgaact taacttccat cttcgacgga
     1081 tgtacgttca catccacctt agccggatga aggtcaatga acaaaaagaa agaaggataa
     1141 tcgcctttct caatgagatg atcaaatccg gaatagactg cataattcag agaccggctg
     1201 acaaagaatc ttccgttaag ataaaaatac tgatcctgtt ttgacttctt tgtaaaatta
     1261 ggtttggaaa taaaacccct tacttttata atttcatttc cgttttccac atcaagtaaa
     1321 tatcccggta tcttatcgcc aaagagttcg ctgatgcgtg tcttcaggtc agttcctttg
     1381 agtctgaaga cttcattatc attattaaaa aaagtaaatg cagtttcagg atgtgacaaa
     1441 gccagtctta caaaggattc gtagatatgt ctgaattcag tctgatcgga tttcagaaaa
     1501 tttcttctgc cgggtgtatt gtaaaaaaga tttcttacag agatagaagt accggtctca
     1561 tttcctgatt tagaattttc ttttacctca ccgccttcta ttctcagcag agtaccgact
     1621 tcagaatctg cagttcttgt gataagttcg gcatgtgaga tcgaagcgat cgaagcaagc
     1681 gcttcacctc tgaatcccag agtgagaata ttttcaagat cttcaatgct cgcaatctta
     1741 cttgtagaat gtctttgaaa acagatcagt gcatcttctt cactcattcc tgatccgtca
     1801 tccgttacct gtatcagact tttaccggca tctttgataa taagtgtaat attctttgca
     1861 ccgctgtcga gagaattttc aataagttct ttcacgacag actcaggtct gttcacgact
     1921 tcacctgctg cgatcttatt ggaaagtgtc tgcggtaata ttttgatctt attttctgtc
     1981 aaatgatttt atgcggtgta attttcttta tgaataattt taacaaattt aatatataaa
     2041 tattgaaatt taattttcct ttcagctgcc ccaccctgcc cttccctttc caagaaagtg
     2101 tctcaaaaaa taattttaat taataccgca aagacactaa cacacaaagc attctttttt
     2161 ttaaagtttt tttctttgtg tctttgtgac taagtagtaa aaatagggtt acttgattca
     2221 gcccttatgt aaattaacta taatttattt ttcaaacaaa gcttccgtaa aactcttgct
     2281 gtcaaatacc tgcagatcat ctatctgctc acctactcct atgaatctta caggaagttt
     2341 catctcttta tttatcttta aaactattcc gcctttggca gtaccgtcca gctttgtcag
     2401 aataattccg gtaagtcctt caagagcttt ggaaaattcc attgcctgat taagtccgtt
     2461 ctgacctgta gttgcatcta tgacaatgaa agtctcatgc ggtgcatcag gaattacttt
     2521 ttgaatgacc ctcttgattt tcttaagctc ttccatcaga tgggatttat tgtgaagtct
     2581 tcccgcagta tcaattatta ctacatcagt attttttgac ttcgcggaat tcactgtatc
     2641 aaatgcaacc gaagccggat ctgaagtgct cttactctga atgatctcga cgtcagctct
     2701 cttcgcccat atatcaagct gatcatttgc agcagcccgg aaagtatctg cagatccgat
     2761 cagtacactc tttcccgctt tcttaaaatt atttgcaagc ttaccgatcg atgtggtctt
     2821 gcctacaccg ttcactccga ccactattat cacataaggt ttttgcttca ggtcaaaatc
     2881 aaatgtctta aattccgatg tcgtctcagt aaacactttt ctgatctcac tgttgataag
     2941 ttcattcagt tctttctgat caatatattt atcagtaaga gccctgtctt ttattctttc
     3001 aataagctgc tcagcagtat ccagtccgag gtcagatgaa ataaatatct cttccagccc
     3061 ggcaaggaat tcatcatcga tcactcccct tgcattaacc agacggttaa cttttcctac
     3121 aagattgtct ttggttttat ttagaccttc ttttaatttt ccaaaaccta atttatctaa
     3181 aaatgccaat ttataaaatt ttatttattt gctgaaatta atctgtaaca aaaaaagcca
     3241 agctcaaaac agagattggc ttttgaataa ttttaatatt ttttaataca tattaagcag
     3301 atttcttgca atcaccattt tctgaacttc agaagtacct tctcctatag tcatgagttt
     3361 tacgtctctg tataatttct ccaccggaaa ttctttcata aatccgtatc cgccgtgtat
     3421 ctgaatggct tcattggttg ctgtggttgc aatttcactt gcataaaatt ttgccatcgc
     3481 agcactgaga ttaatttcca ttccattatc tttcatccat gcagctttgt aaaccatcag
     3541 tctggctgca tctgcctgag tcgccatatc tgacagctta aattgtattc cctgaaaatg
     3601 cgaaagagat tttccaaact gctttctctc tttagaatat tttaatgaag catcaagtgc
     3661 tccctgagca attccaagtg aaagcgctgc aatagcgatc ctgccgccgt cgagaatttt
     3721 catacactgt ttgaatccct gaccttcttc accaataaga ttttccgcag gcacttcgac
     3781 attttcaaaa attaattcgg cagtatctga agatctcatt ccgagtttat tttctttttt
     3841 acctgtagta aaacctttca tacctttttc cagaatgaaa gctgaaatgc cgttcttctt
     3901 tttttcttta tctgtgacag cagttacaac aaatgtttct cctacacttc cctgagttat
     3961 aaaaagttta ctgccgttta taatataatg accattcttt ctttcggcag tggtcgccat
     4021 tcctgctgca tcacttcctg aaacattttc tgtcaatccc catgcgccga gtttcttacc
     4081 ggtagtaaga tcgggcagat attttttctt aagttcctca tttgcaaaac tgtaaatatg
     4141 attagtgcat agtccgttat gtgctgcaat agtcagcccc attgacggat ctacttttga
     4201 gatctcttct actatgatcg catattccag agtcgaaaat cctgcgcctc cgtactcttc
     4261 aggaaatatg attccgagaa atccaatgtc accgagcttt tttgcaattt ccatcgggaa
     4321 ttcctgtgac tcatcatatt tcataacatg aggtgctatc tcattctgcg caaattctct
     4381 tgcaagtttc tgtacagcaa gttcctgttc agataaatta aaatccatat ataatatatt
     4441 ttctgattat ttaaaaatag agttgtaaat atagtgatat taatttttta ctgaaatgtt
     4501 aaataagagt atctttgaaa tttatgaccc tacaaaaaat attaattgtt ttttaaaaac
     4561 agtaatcatt atatttgcat caaaaaaaat taatatgaaa ttgaatatca taaagaattt
     4621 tgcagcagtt atgttaattt ttagttttac aattcttttc atttcctgca gtaaagaaac
     4681 tccgcagaca tattttaatg tatctgtatt aaattcaaat atcctcagcg gctttgccgg
     4741 cactggattt tcacgtcaat tatcttcttc ttctgaaaaa ttaaaggaaa acggaactga
     4801 gaccgaacct atgaagagaa gcgaagtaat aaatgataag atcggtcaaa tagaagaata
     4861 ttataataat gtaagttcac ttaataaaac cgacgataca aaagaaatga tcgatgcttc
     4921 tttgaattta tacaattttg ttttaccggt ttgcaaaaca gaatatagga agcttgcaga
     4981 gctttatgat aacaatgctt cacagtctga tatcgataaa ctaactttgg aaattcagga
     5041 taaatatctg cagaaatata ccgatcttta ttctcaattg ataaaaacag gaaaagtgta
     5101 tgcggaaaag aattctataa aagtaaactg ggcagatgaa taataattaa ttacgataat
     5161 taaattttat tgtgatttat tatttcaaat tattataaat attcttcaat tcagtttttc
     5221 atttttaata taagctctta cttttatatt gtcaaaatta aa
//
LOCUS       DAORVQ010000221         5220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10937_length_5220_cov_5.906873, whole genome shotgun sequence.
ACCESSION   DAORVQ010000221 DAORVQ010000000
VERSION     DAORVQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10937_length_5220_cov_5.906873"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLX80_13010"
     rRNA            complement(<1..333)
                     /locus_tag="PLX80_13010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1071..2750
                     /locus_tag="PLX80_13015"
     CDS             1071..2750
                     /locus_tag="PLX80_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21755.1"
                     /translation="MKTVKVIFSWLFLFSSLSISDVISQQYQVSQNTYNSSANSINLN
                     SKVKFDKMGNYYVMCISEDPGSGMDFTILKYSSAGPLLWDYKFNGAMNGRDEPLDFCL
                     DNEGSVYVTGKSQTSTFINSCATIKLSSSGLMMWNKVLDNTSDIQSEGNSIALDEAGN
                     IHVAGSAGNPKEILLIKYNTHGEILHQVRYNQRKSSNSIAYDIKIKNNKIIVCGESTQ
                     PDQDYDLMISCFDLDGKFNWMRLHNGNANTRDMASSVHIDNNNNIYSTGFLESNLGKQ
                     DIVTLKYDMNGNLIWKTEFNGTANGYDWPNHVLTDSKGNAYVCGGSQNTRVDDSECYS
                     DLGKCQNDATVIKYRPDGVIDWVRIYNGTENMYDCLQGMVIDRNDNIYASGRSHNNLT
                     NGDDDMIFFKYNTSGDLLSFNRFNSGSSNAEYGNDIDVDLNGNVVAVGSIWQNNLYNL
                     LAVKHKPTTIISNDNNSSGTFKAKAYPNPFNPSTVITFNVPAGADNTGLPVNIVVFNS
                     LGKEVAQLTNEIMNPGIHEIRFDGSNLTNGVYFYRITAGNNIEINKLMLIK"
     gene            3124..4206
                     /locus_tag="PLX80_13020"
     CDS             3124..4206
                     /locus_tag="PLX80_13020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY21756.1"
                     /translation="MEKTKKKYYHHKRKKPFNKTNDQENSADKSENFNSKVTKTEEKI
                     PPHRILNPNQKISVVIPLYNEEDSLQELSDSLKRTFDSLKCNYEVLFIDDGSTDNSFQ
                     KIKEINRKDNNFKCIKFRRNYGKSAALSKGFKAAKGNIIITMDADLQDDPSEIPELLK
                     VLNSGYDLVSGWKKVRYDPFIKKHTSKIFNYFTSKLSGVRLHDFNCGLKAYKRDVIKS
                     VKIYGEMHRYIPALAHLSGFKVTEKPVKHHARKYGVTKFGASRFVNGFLDLLTVSFTN
                     KYMKRPLHFFGSLGILISMAGFFISFYLVVLKFLEGMPLSDRPLFLVGIFMMVVGIQF
                     LSLGLIAEMITKSNISDDDILIEETI"
     gene            4239..5003
                     /gene="lpxA"
                     /locus_tag="PLX80_13025"
     CDS             4239..5003
                     /gene="lpxA"
                     /locus_tag="PLX80_13025"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQY21757.1"
                     /translation="MSNSVISSKADIGNNFSIKNFSVIEDDVVIGNNVEIGSNVLIAN
                     GARISDNVKIHHGAVISTIPQDLKFEGEVTTMEIGDGTVIREYATLNRGTNYSNKTVV
                     GSNCFIMAYAHVAHDCIIGDRVIIANSVQMGGHVEIGDWTIIGGLVPIHQFTKIGAHV
                     MIGGGFRTVKDVPPYILVGNLPLRFEGVNIVGLRRRGFTNEQIYNIKDAYDVIYKSGF
                     NVSDAVKEITAKGNVPDEVKLILDFIEKSDRGIIRG"
     gene            5006..>5220
                     /locus_tag="PLX80_13030"
     CDS             5006..>5220
                     /locus_tag="PLX80_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21758.1"
                     /translation="MFDKNYEIKCKIPDIAKFRNIIKKESSYIYSKETQTDIYYRVKK
                     GRLKLRIINNEIASLIYYERSDKTKIRT"
BASE COUNT         1869 a          758 c          870 g         1723 t
ORIGIN      
        1 gaccgtgtct cagttccagt gtggctgatc atcctctcag accagctact gatcgtaggc
       61 ttggtgggct attactccgc caactaccta atcagacgca ggctcatctt caggccccga
      121 aggtttaaca actgtctcga tgtcgagccg ctgcattatc tgatattagt cccgctttcg
      181 cggaattatg tcagtcccaa aggcagatta cctacgtgtt actcacccgt gcgccacctc
      241 ccttccagta ttgctaccaa aagttcagtt gacttgcatg tattaagcac gccgccagcg
      301 ttcgtcctga gccaggatca aactctccat tataatttta taactttgat ttgatcgtag
      361 ctacaaggtt atttaaggta tcgaaatcta cacactaaat tttattaaac tattattaaa
      421 attttattac ctgtgcaaat tttatgtaaa agaacattta aattacgaac tacaaatata
      481 cgagatttct tttgataagt caatggatat ttctgtgtag cctaaaatat ttttgatttt
      541 tttctgagaa gctgtataaa attcgtgtct gccaatataa caattttaat ctaatataaa
      601 aattaacttt tgtatacatt cttaaaaaaa ttctgtaaat atatttttca tatattctca
      661 tatattctta caagattttc tatttctata aacttaaaaa aattatcccg atattgactt
      721 cttgctttgt tatttttttt attcaatgag tatccttgtc gtgtatactc ttcaatactg
      781 ctatcacggt gctttgctta cacagattta aattcaaatc aaaattattt ttgttatatg
      841 tatttttaca aattgaatca aattgtatat ctaaataaaa atttcacttt ctaagccgta
      901 ggttgattat taaatttgga tttaatattt tacaataact ttgaagacta tatgaatcat
      961 caaaaaattc ctgcgtatat tatctatcat atttcttgcc tcactgaaac taaacaaaaa
     1021 gactgtatcc gaaattttca aaaaattttt ttaaacttaa ataaaaaaat atgaaaaccg
     1081 taaaagtaat tttcagttgg ttatttcttt tttcatcttt atcaatttca gatgtcattt
     1141 cacagcaata tcaagtaagc caaaacactt ataactcatc ggcaaattca ataaatttaa
     1201 actccaaagt taaatttgat aaaatgggaa attattatgt tatgtgtatt agtgaagacc
     1261 ccggttctgg aatggatttt actattttaa aatattcttc cgcaggtcct cttttatggg
     1321 attataaatt taatggtgct atgaatggca gagatgaacc tttggatttt tgtctcgata
     1381 atgaaggcag tgtttatgtg acaggaaaaa gtcagaccag tacttttata aattcttgcg
     1441 caacaattaa attaagtagt tcaggtttaa tgatgtggaa caaagtttta gacaatactt
     1501 cagacattca aagtgaagga aattcaattg ctcttgatga agcaggtaat attcacgttg
     1561 ctggttccgc tggaaatcca aaagaaattt tgttaataaa gtataatact catggagaaa
     1621 tattacatca ggtaagatat aatcaaagga agtcaagcaa ttcaatagct tatgacataa
     1681 agatcaaaaa taataaaatc attgtttgtg gtgaatcaac acaacctgat caggattatg
     1741 atttaatgat ttcatgtttt gatctagacg gaaaattcaa ctggatgaga ctgcataatg
     1801 gaaatgctaa tacaagagat atggcatctt ctgtacacat tgataataat aataatattt
     1861 attctactgg ttttttggaa agtaatttag gtaagcagga cattgtaact ttaaaatatg
     1921 atatgaatgg aaatttgatc tggaaaactg aattcaacgg aactgctaat ggatatgact
     1981 ggccaaatca tgttcttact gattccaaag gaaatgctta tgtttgcggt ggaagtcaaa
     2041 atacaagagt agatgattct gaatgttatt ctgatcttgg taaatgtcaa aatgatgcaa
     2101 ctgtaatcaa atacagacct gacggtgtta ttgattgggt taggatttat aatggtacgg
     2161 aaaatatgta tgactgctta cagggaatgg tgattgaccg caatgataat atatatgctt
     2221 ccggaagaag tcataataat ttgactaatg gagatgatga catgattttt ttcaaatata
     2281 atacatcagg agatttatta tcattcaaca gatttaacag tggctcaagt aatgctgaat
     2341 atggaaacga tattgatgtg gatctcaatg gtaatgtcgt tgcagttgga tcaatttggc
     2401 aaaacaactt atataattta ttggcagtta agcataaacc aactacaata atcagcaacg
     2461 ataataattc ttcaggtaca ttcaaagcaa aagcttatcc aaatccattt aaccctagta
     2521 ctgttattac ttttaacgta ccagccggag cggacaatac agggttacct gtaaatatag
     2581 ttgtatttaa ttcacttgga aaggaagtcg ctcaacttac aaatgaaatt atgaatccgg
     2641 gaattcatga aattagattt gacggatcaa atttaacgaa tggagtttat ttttatagaa
     2701 taaccgcagg taataatatt gagatcaata aacttatgtt aataaaataa tttacttttt
     2761 atttctgaca tttttttatt taattattac tttttcaaaa aatatttttg aaaattttta
     2821 ataatatttc cccacttgtg aaaatatact tacctaaatt cgcaagcggg gttttttata
     2881 acatttcaat ttatatcagt aaatttaaca tcagaattga aacgaaataa tttttaaaat
     2941 ctttatacat taatgaattt attaccatat taatttgtaa tttctgaata ttctaaattt
     3001 tatgggtttt caaaataaaa taggaaattt taaattcaaa tgtcactata ggcgttgcta
     3061 attttcaatt gattttagat tcgataaaag ttagtttatt taccaattgc aaacatttta
     3121 ttgatggaaa aaactaaaaa aaagtattat catcacaaga gaaaaaagcc ttttaataaa
     3181 accaatgatc aggaaaactc tgcagataaa tctgaaaatt tcaatagtaa agtaactaag
     3241 actgaagaaa aaattcctcc gcatagaatt ttaaatccta atcagaaaat ctctgtagtc
     3301 atacctttat ataatgaaga agattctttg caggaattaa gcgattccct taaaaggaca
     3361 ttcgacagcc ttaaatgtaa ttatgaagta ctatttattg atgacggaag tacagacaat
     3421 tcttttcaaa aaataaaaga aataaacaga aaagacaata atttcaaatg tataaagttc
     3481 agacgaaatt atggtaagtc tgcagcactt tcaaaagggt tcaaagctgc taaaggtaat
     3541 atcataatta ctatggatgc tgatcttcag gatgatccgt ctgagattcc cgaactttta
     3601 aaagttttaa actccggtta tgatctcgta tcaggctgga aaaaagtcag gtacgatcca
     3661 tttataaaaa agcacacatc taaaattttt aactatttta catcaaaatt atccggagta
     3721 agattacatg attttaactg cggtttaaaa gcttacaaaa gagatgtaat caaatctgta
     3781 aaaatatacg gggaaatgca cagatacata ccggcactcg ctcacctgtc tggatttaaa
     3841 gttactgaaa aacctgtaaa acatcatgcg cgaaaatacg gcgttacaaa atttggagca
     3901 agtagatttg taaacggatt ccttgatctt ctgacagttt catttacaaa caagtatatg
     3961 aaacgtcctt tacatttttt tggaagtttg ggtatattaa tttcaatggc agggtttttc
     4021 atatcttttt atctggttgt tctgaaattt ctcgaaggaa tgccattaag tgacagacct
     4081 ctctttttag ttgggatttt tatgatggtg gtcggcatac agtttctttc acttggattg
     4141 attgctgaaa tgataacaaa atcaaatata agtgatgatg atattctgat cgaagaaaca
     4201 atttaattat ttaataaaat ttttataaaa agtatttaat gtctaattct gtaataagca
     4261 gtaaagctga catcggaaat aatttcagca taaaaaattt ttctgtcatt gaagacgatg
     4321 tagtcattgg gaataatgtg gagataggtt cgaatgtact catcgcaaac ggtgcaagga
     4381 tttcagataa tgtaaagatt catcacggag ctgtgattag tactattccg caggatctaa
     4441 aattcgaagg cgaagtgact actatggaga tcggagatgg aactgtgatc cgtgaatatg
     4501 ctacacttaa cagaggtaca aattacagta acaaaacagt tgtgggcagc aactgtttta
     4561 taatggcata tgctcatgtt gctcacgact gtataatcgg agacagagta ataattgcaa
     4621 acagcgttca gatgggcgga catgttgaga tcggtgactg gacaattatc ggcggacttg
     4681 ttccgataca tcaatttaca aagataggag ctcacgtaat gataggcgga ggattcagaa
     4741 cagtgaaaga cgttcctcca tatatacttg taggaaattt accattgaga tttgaaggtg
     4801 taaatattgt cggtcttaga agaagaggtt ttacaaatga acagatatat aatattaaag
     4861 atgcatatga tgttatttac aaatccggat tcaatgttag cgatgctgta aaagaaataa
     4921 cagcaaaagg aaatgttccg gatgaagtaa agttaattct tgatttcatt gaaaaaagtg
     4981 accgcggcat tatcagaggc tgagattgtt tgataaaaat tatgaaataa aatgtaagat
     5041 acctgatatt gcaaaattca ggaatatcat taaaaaagaa agctcttaca tttactcaaa
     5101 agaaacacaa accgatattt attatagagt aaaaaaagga agactcaagc tgagaattat
     5161 caataatgaa attgccagtc ttatttatta cgaaagatca gataagacaa aaataagaac
//
LOCUS       DAORVQ010000222         5211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_10975_length_5211_cov_7.657486, whole genome shotgun sequence.
ACCESSION   DAORVQ010000222 DAORVQ010000000
VERSION     DAORVQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_5211_cov_7.657486"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PLX80_13035"
     CDS             complement(<1..1403)
                     /locus_tag="PLX80_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY21759.1"
                     /translation="MKSFILIFTFLLAVGNISIAQDMVNTKNTATERAPKSITVTENN
                     NNNQISAFGGRWFGQNAAATSFFARGVLNTTGSLSNFGPGSTSLFGGMEFSNTGTLYC
                     IAVAAASPLQTLDTVTGALTTLGAITGIGSEQVLSLSFNNANNTMYLTTTVTGGDKLY
                     TLDLTTRVATLVGNAGQNFFGIAINSTGLCYAISIDDNLYSINLTSAAGTLIGPIGFD
                     ANFIQGLSFDRQTDTLWYAAYNNTALRGELRTVNLTSGATNLIAPFNPAAEVCGFAIP
                     SSAPPPPVGGSITITRNQYVQIPDNGGNANPAVDNIDISGIAGNVEIKKVTLTINNVV
                     QSWVGDLRFWLTKGNSVDTSISRIGWTGTGFGNSCDNLTGTVLSDSAGLTNIQNIPTV
                     CVGGLAQATGNFNPKEPFSQFQNSGVNPNGTYTLRISDNAAGDTGSLRSWTLKIDYDI
                     ITGVNNNVTSIANDYSL"
     gene            complement(1538..3331)
                     /locus_tag="PLX80_13040"
     CDS             complement(1538..3331)
                     /locus_tag="PLX80_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21760.1"
                     /translation="MSEKAAHKGEEDTLTKDIDSKILKRLLGFFKPYSRYIVFATFLT
                     IIISALAALRPRLTPIAIDDKIMNNDAPGLQYIILIMLGTLVVQGIIQYGMTYLTSWI
                     GQKIIYDLRKKIYSHILSLDLKFFDRNPIGRIVTRVTGDVEVLFEVFSSGLVTAFGDI
                     FLIFWILYFMFSLDFTLTLVTLSVLPLLIYATSVFRKKVRESYRRIRLLIAKLNSYIQ
                     EHLTGITIVQYFVKEKKTIDEFEEINRDHTEQNKKSVLYYAVFFPIVELLSAISAGLI
                     IWYGGGQVIQNAVSVGVLISFIQFTEMFFRPIRDLSEKYNILQTAMASSERIFALLDE
                     KPTISDPEKPIELKDVKGNIEFRNVSFAYNEGDYVLRNINFKIKEGEKVAFVGATGAG
                     KSTIINLLTRFYDVYEGEILLDGVNIKDIEQMELRKNIAIVLQDVFLFSGDIRSNITL
                     NNPEITDEIIYNAIDNTGLRPFVDSHPETIYKQVTERGSSFSVGQKQLISFARALAYD
                     PKILILDEATSSVDTNTEILIQNAIKKLIEGRTSIIIAHRLSTIQNCDKIIVMHKGEI
                     KEMGTHQQLLEKGGLYFKLYQLQYKEEEISE"
     gene            complement(3417..5168)
                     /locus_tag="PLX80_13045"
     CDS             complement(3417..5168)
                     /locus_tag="PLX80_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21761.1"
                     /translation="MKHLYSLLPYLKKFKKKIYSGFFFIILSNASDSLYPLVIGMAID
                     DLSKGTLRHNLLTYVLVGLLIVVLRGTFLFLTRQTLIVVSREIENDLRFDFFSHLQRL
                     SKSFYNNRSTGDLMAHATNDISNVRNFVGPGIMYSFQTFTRILFTLIILFSMSSSVTL
                     LALAPLPLITYIVYKVGKLTFNRSRKVYKIFSDLTSKAQECFSGIRVVKSYVRERNEN
                     REFDKISFDYQKKNLSLAKVQAFSFPMMFMLTSLSVIIVIYFGGIKIMEGSMSLGDLS
                     AFLIYLGQLTWPMIAFGWIINLIQRAAPSMERLLSIINVKPDIADNENTDEDITQKDI
                     EGEIEFENVSFKYPLSNNFVLKNINLKIRKGTTLGIIGHTGSGKSTLISLIPRIWDVT
                     EGRILIDGMDIKKIPLDVLRRSVGIVPQESFLFSTTIEKNVSYSSDFIDEDEMISAAK
                     KSGLYKDVDEFPLKFKTVLGERGITMSGGQKQRTSIARAIYKKPEILILDDSLSAVDT
                     KTEEEILKELKSVMRDRTSLIISHRISTIKNANNIIVLSHSKIAEEGTHNELISAGGI
                     YFDLYKKQLLEEEIKEF"
BASE COUNT         1531 a         1039 c          932 g         1709 t
ORIGIN      
        1 cttaagctgt aatcattggc aattgaagtg acattattat taacacctgt aataatatca
       61 taatcgatct ttaatgtcca gcttctcaga cttcctgtat ctcctgcagc attatctgaa
      121 attctgagtg tatatgttcc attcggatta acaccactat tttggaattg tgagaatggt
      181 tcttttggat taaaattacc tgttgcctgt gctaatccac caacacaaac agttggaata
      241 ttttgaatat ttgtcaaacc tgctgaatca cttaaaactg tacctgttaa gttatcacaa
      301 ctgtttccaa atcctgttcc tgtccatccg attctgctga tagatgtatc tacagaatta
      361 ccttttgtta accagaatct gagatcaccg acccagcttt gtacaacatt attgatagtt
      421 aaggtaactt ttttgatctc aacatttcca gcaattccac tgatatcaat attatctaca
      481 gcaggatttg cattacctcc gttatcagga atctgaacat actggtttct agtaattgta
      541 attgatcctc caacgggtgg cggtggtgca gaagacggaa ttgcaaatcc acagacttca
      601 gctgccggat taaatggtgc aattaagtta gtcgcacctg atgtaagatt tacagttctt
      661 aattcacctc ttaatgcggt attgttatat gcggcatacc atagagtatc agtctgtctg
      721 tcaaagctca aaccctgtat gaaatttgca tcgaatccaa taggtccgat caaagtacct
      781 gcagcagatg taagatttat gctgtataaa ttatcatcaa tacttattgc gtaacaaagt
      841 cctgtactgt ttattgcaat accaaaaaag ttttgacctg cattaccaac aagtgtagca
      901 actcttgtag tcaggtctag agtgtaaagt ttgtctccac ctgttacagt agtagtaaga
      961 tacatagtat tattggcatt attaaatgaa aggcttaaaa cctgttctga accaattcct
     1021 gtaattgctc cgagagttgt taaagctccc gttactgtat caagagtctg aagcggtgaa
     1081 gcagcagcta cagcaataca atataatgtt cctgtattac tgaattccat tcctccgaaa
     1141 agacttgtac taccgggacc gaaatttgat aaagatcctg ttgtgttgag aactcctctt
     1201 gcgaaaaaac ttgtagcagc agcgttttgt ccaaaccatc ttccgccaaa tgctgaaatc
     1261 tgattattgt tattgttttc tgtaactgta atacttttcg gagctctttc tgtagctgtg
     1321 tttttagtat ttaccatatc ctgagcgatc gaaatattac cgactgctaa aagaaaggta
     1381 aagatcagaa taaatgattt catttttctc tggttaattt ataaatagtt aataaaagtt
     1441 ataaaaattt tatggttgga aattatttta ggttcaaaat atttaataat aaagttttaa
     1501 acaacatact tgaaattatt tttatctcaa atacttatta ttccgaaatc tcctcctcct
     1561 tatattgcaa ctgatacagt ttaaaataaa gaccgccctt ttccaaaagc tgttgatgtg
     1621 ttcccatttc tttgatctcg cctttgtgca ttactattat tttatcgcag ttttgaattg
     1681 tcgaaagtct gtgagctatg ataatggaag tcctgccttc tataagtttt ttgatcgcat
     1741 tctgaatcag aatttcagta ttggtatcga cactcgaggt ggcttcgtcc agtatcagaa
     1801 tcttcggatc ataggcgagc gctcttgcaa atgaaatgag ctgcttctgt ccgactgaaa
     1861 atgatgatcc tctttcagtc acctgtttat aaatagtctc aggatgcgaa tcaacgaaag
     1921 gacgcagacc tgtattatca atcgcattat aaattatctc gtcagtaatt tcaggattgt
     1981 tcagagtaat atttgatctg atgtctcctg aaaaaagaaa tacgtcctgc agtactatgg
     2041 caatgttttt tcttaattcc atttgttcaa tgtctttaat attaacacca tcaagaagaa
     2101 tctcgccttc atacacatca taaaatctcg tgagcagatt tattatcgtt gacttacccg
     2161 caccggtagc accgacgaat gcaaccttct cgccttcttt tattttgaaa ttaatattcc
     2221 tcaaaacata atcgccttca ttatatgcga agctcacatt cctgaattcg atatttcctt
     2281 ttacatcttt aagttctata ggcttctccg gatctgatat tgtcggcttt tcatcgagca
     2341 aggcgaagat cctttcgctc gatgccatag cggtctgaag tatattatac ttttccgaaa
     2401 ggtctctgat cggacggaag aacatttccg tgaactgaat aaatgagatc agcactccga
     2461 ccgaaacggc attctgaatg acctgaccac ctccgtacca gattatgaga ccggcggaaa
     2521 ttgcgctgag cagttcgaca atcggaaaga atacagcata atacaaaaca ctctttttat
     2581 tctgttcagt atggtcacgg tttatttctt cgaattcatc aatcgtcttt ttctctttta
     2641 caaaatactg tacaatggta attcccgtca gatgctcctg tatgtaagaa ttgagttttg
     2701 ctatgagcag ccggatcctt ctgtatgact ctctcacttt ttttctgaaa acagatgtag
     2761 cgtagatcag caaaggcagc actgaaagtg tcacaagcgt aagagtaaag tcgagtgaga
     2821 acataaaata aagtatccag aagatcagaa atatatctcc gaatgcagtg accaatcccg
     2881 atgaaaaaac ttcaaacaaa acttcgacat caccggtcac tctcgtgacg atcctgccga
     2941 taggattcct gtcaaaaaat ttcaggtcaa gtgaaagtat gtgcgagtat attttttttc
     3001 gaaggtcata aataattttc tgtccgatcc agctcgtgag ataagtcatg ccgtattgta
     3061 taattccctg aactacgagc gtccccagca tgatcagaat tatatactgc agtccgggcg
     3121 catcattgtt catgatcttg tcatcaatgg cgataggtgt cagacgaggt ctgagtgctg
     3181 ccagtgctga gatgatgatc gtaagaaatg tagcaaagac aatatatctc gaatatggct
     3241 taaaaaatcc aagcagcctt ttaaggatct tggaatcaat gtccttggtt aatgtatcct
     3301 cttcgccttt atgtgcagct ttttctgaca ataaaaattt actgttattt aaaaataatt
     3361 aaacatctct ttgttcccgc agagtcacat tctctttgga ctctgcggat aatgaatcaa
     3421 aattccttga tctcttcttc tagcagttgt tttttataaa ggtcaaaata aattccgccc
     3481 gctgagatga gttcattatg tgtaccttcc tcagctattt tggaatggct tagcactatg
     3541 atattatttg cattctttat agtcgatatc ctgtgtgaaa ttattaaact cgtcctgtca
     3601 cgcattacac tttttaattc tttcaggatc tcctcttccg tctttgtgtc aactgccgaa
     3661 agtgaatcat caagaataag tatctcgggt tttttataaa ttgcccgcgc aatggaagtt
     3721 cgctgcttct gcccgcctga cattgtgatg cctctttcgc caagtactgt tttaaatttc
     3781 agcggaaatt catcgacgtc tttatatagt cccgattttt tggctgctga tatcatttca
     3841 tcctcatcga taaaatcact cgagtacgat acattttttt cgatcgtagt ggaaaaaaga
     3901 aatgactcct gaggtacgat ccctactgat cttcgcagta catccaaagg tattttttta
     3961 atgtccattc cgtcgatcag gatcctgcct tctgtcacgt cccatattct cgggatcaga
     4021 ctgatgagcg tactctttcc cgaaccggta tgtccgatga tgccgagagt agtgcctttg
     4081 cgtattttta gattaatatt cttgagtaca aaattattcg acagcggata tttgaaagaa
     4141 acattttcaa attctatttc accttcaata tctttctgag taatgtcttc atcggtattt
     4201 tcattatcgg caatatcagg ttttacattt atgatactca aaagtctttc catcgaaggc
     4261 gcggctcttt gaatcagatt gatgatccat ccgaatgcga tcatcggcca tgtgagctgt
     4321 ccgagatata tgagaaatgc tgacagatct ccgagcgaca tacttccttc cattattttt
     4381 atacctccga aataaattac aatgatcact gaaagacttg tcagcataaa catcatcgga
     4441 aatgaaaatg cctgtacctt tgccagactc agattctttt tctgataatc aaatgagatc
     4501 ttatcgaatt cacgattttc atttctctct cttacataag acttgacgac tctgatacct
     4561 gagaagcatt cctgtgcttt agatgtcaga tcggaaaata ttttatagac ttttcgtgaa
     4621 cggttgaatg taagcttccc taccttatat acaatgtaag tgatcagcgg cagcggtgcg
     4681 agcgcaagta atgtcactga cgaactcatc gaaaataaaa tgatcagcgt aaaaagtatt
     4741 cttgtaaatg tctgaaaaga atacatgatc cccggtccga cgaaatttct gacattactg
     4801 atgtcatttg tagcatgcgc catcaggtcg ccggtggatc tgttattgta aaatgatttt
     4861 gaaagtctct gcagatgtga gaagaagtca aagcgaaggt cgttctcgat ctcccgtgat
     4921 actactatca gagtctgccg tgtaagaaaa aggaaagtac ctcttagcac tacaatcaaa
     4981 agtccgacga gaacatatgt cagcagatta tgtctgagtg tacctttact aagatcgtct
     5041 atcgccattc cgatcacaag cggataaaga ctgtccgatg cattagaaag tattataaaa
     5101 aagaatccgc tgtagatctt ttttttaaat ttctttaggt atggtaaaag ggaatataga
     5161 tgcttcatcg gataaaataa tgatgaatat aacttttaat aattttaatt t
//
LOCUS       DAORVQ010000223         5167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_11157_length_5167_cov_6.060250, whole genome shotgun sequence.
ACCESSION   DAORVQ010000223 DAORVQ010000000
VERSION     DAORVQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11157_length_5167_cov_6.060250"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="PLX80_13050"
     CDS             complement(<1..2486)
                     /locus_tag="PLX80_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY21762.1"
                     /translation="MILIKKAGGSKQFSRGGIIVVCLIFIYLFSAFTFQIQDSLPVHE
                     KIDTSLINFDSIRLETFNTDSVKKYFKATDTLISYQLNKIEEYTLALNKINSTLRRGF
                     DTTSISNGLPETDSIISVAKINLKGFRGQTSLKILIATKNILLTLQNRLSAWQDELTI
                     YNNNLNSIKEKLKKISRDQYLLIMPEDSILFAEYIKKLKPLGQKTNAADSLTAVQLKK
                     LGVTQNRVSSNYIDVTDLLDDVNFHINNFSSKILDREFGYLWDSGTESDDEPGFDEVV
                     EKSFKESKLLLRMYLTSNWLSRIIVLLIGILFYFLMRNKINLIKKESSDPEAVLAKAN
                     HIPRHLFTSTVVFSLMLIPFIYSTAPQAFTLSLWGIQIIALAFLIRDKFKFISGIQLL
                     ILLILFYLCGFSNLLIETTTTERWVQLLISTVSVILGLWYLKKNNLNHFSKTVIHRPI
                     IIIFIGMNAAALLLNIFGRVTLAKILNTGASYGIIEAIILLVFVEIVIDAVYLIMESS
                     RKSSRLTAYFEFKGLENRLRNILGILAGVVWLVLFTKNLHIYDFIFGGISEFLSAVHQ
                     IGDIKFSFGSIVIFGIVIWVSTLISQMVAFVFGGPETDSSTNDTHKLGSTVLLIRLAI
                     LALGVLLAFGASGIPLDKLAIIIGALGVGIGFGLQNIVNNLVSGIVIAFEKPVQIGDV
                     IEVGTRIGTVKEVGIRSSTILAFDGSEVIIPNGDIISQHLVNWTKDSKFRRIEIPVGI
                     AYGSDINKVKETVLDVFTNKDQIIKFPVPFVRVLNFGDSSIDLKIYFWAEVHNWIDIK
                     SDIMAEVYDRLNEAGIEIPFPQRDLHVRSV"
     gene            complement(2892..3665)
                     /locus_tag="PLX80_13055"
     CDS             complement(2892..3665)
                     /locus_tag="PLX80_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21763.1"
                     /translation="MHREEILTEILKELRSYPQVNLIAEGGAKAFNRYDELSDIDLMV
                     DTEDGTAGETVKKFEDFLEELTGINSVYNVHENSGINDRQHKFYKLKDTDKFSIVDLF
                     IVERSNPMKEMEKHIHGDLVIHYDKYDYMKDQTYDKELLQKKIDEFEYKYKSVFEFFL
                     YQTEKEIIRGHYIDALAYYFDLTLKPLIRSLRVKYNPVHYNFELRYLYDELPEYLVKE
                     IESLLSIADIDDLKIKLKMAEELYRREIKSIKNPESFRL"
     gene            complement(3714..4898)
                     /locus_tag="PLX80_13060"
     CDS             complement(3714..4898)
                     /locus_tag="PLX80_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HQY21764.1"
                     /translation="MIRENFNKLIKSIAFYPALIALGFLLLSYFSIAFDFSESGQHIR
                     SNLKWLKLQDPSTARSIISSIAAGIISLTVFSFSMVMIVLNQTASQMSNRILDNLIGS
                     RYQQMVLGIYVGTIVFALFLLSSIRDIDSGLQIPVLSTYLLMLLTVLDVFLFIYFLHY
                     ITQSVKYEVIIRRIYKETREALEQSCISNTEPAEIFHSVSDHIINSQCPGIYEGFNKN
                     SFISICEKNECMLYLIHTPGTFILKGMPVIKSNKELPETLKTELINSLYIHESESIKN
                     NFYFGCRQLTEVAVKALSPGINDPATAVSSLRALFELYLYRASNYPETYIRTESDKAG
                     IILKELTFEKIFTATLLPIWDYGKDDRMIREEMHHLLINLKSLTGKTVISDIIKKVEN
                     KF"
BASE COUNT         1595 a          963 c          809 g         1800 t
ORIGIN      
        1 tctaccgatc tgacatgcag atctctctgc ggaaacggga tttcaatgcc ggcttcattc
       61 agcctgtcat atacttctgc cattatatcg cttttaatgt ctatccagtt atgaacttca
      121 gcccagaaat atatctttag atctattgaa ctgtccccga aatttagtac tctgacaaat
      181 ggtactggaa atttaattat ctgatcttta tttgtaaata catccagtac agtttctttt
      241 actttgttaa tgtccgaacc gtatgcaatt ccaaccggaa tttctattct tctgaattta
      301 ctatccttgg tccagtttac aagatgctgt gaaattatat ctccgttggg gataattact
      361 tccgaaccgt cgaatgcaag tatagtactt gaacggatac ctacctcctt tacggtaccg
      421 attcttgtac ctacttctat tacatctccg atttgtaccg gtttttcaaa tgctatcaca
      481 attcccgata ccagattatt tacaatgttt tgtaatccaa atccgatacc tactccaagc
      541 gctccgataa ttatcgccag cttatcgagc ggaattcccg atgctccaaa tgcaagtaat
      601 accccaagtg caagtattgc caggcgtata agcagtactg tcgatccgag tttatgagta
      661 tcatttgtgc tgctgtcggt ttcaggtccg ccgaaaacaa acgcaaccat ctgtgagatc
      721 aaagtcgaga cccatatcac aattccaaat attactatac ttccgaaact gaattttatg
      781 tctcctatct gatggacagc tgataaaaat tctgatatcc ctccgaatat aaaatcatag
      841 atatggagat tcttcgtaaa caaaaccagc cacactacac cggctaaaat tcccagtata
      901 tttctaagcc tgttctccag acctttgaat tcaaaatatg ccgtgagtct tgaacttttc
      961 ctgcttgact ccataatcag gtaaactgcg tctatgacta tttctacaaa caccagcaaa
     1021 ataattgctt cgattatacc gtaactcgct cccgtattca gtatctttgc aagtgttact
     1081 cttccaaata tgtttaataa cagtgcagcg gcattcattc ctatgaaaat gattatgatc
     1141 ggcctgtgaa ttactgtctt tgaaaaatga tttagattat tctttttaag ataccaaagg
     1201 cccagtatta cagatacagt actgattaaa agctgtaccc atctttccgt tgtcgtcgtt
     1261 tcaattaaca ggtttgaaaa tccgcaaaga taaaaaagga ttaaaagtat taacagctgt
     1321 attcctgata taaatttaaa tttatctctg atcaaaaatg ccagcgcaat gatctggatt
     1381 ccccaaagtg aaagtgtaaa tgcctgaggt gcagtggaat atataaatgg tatcagcatc
     1441 agactgaaga ctactgttga agtaaacaga tgcctcggta tgtggttggc ttttgccaaa
     1501 accgcttcgg gatcactgct ctcttttttg atcagattaa ttttatttct cattaggaaa
     1561 taaaacaaga ttccgatcag caatactatt atccgggaaa gccagtttga ggtcagatac
     1621 atccttagaa gtaacttact ttccttaaac gatttttcta caacttcatc aaaaccgggt
     1681 tcgtcatcag attcagtccc gctgtcccat aggtatccga attccctgtc taatatttta
     1741 ctgctgaaat tgtttatatg gaaatttaca tcatccagaa gatcagttac atcaatataa
     1801 ttgctggata ctctgttctg tgtaacacca agtttcttta attgtaccgc tgttaacgaa
     1861 tcagcagcat tcgtcttctg accgagtggt tttagttttt taatatattc tgcaaataga
     1921 atactgtctt cgggcatgat tagcagatat tgatccctgc tgattttctt cagtttttcc
     1981 ttgatcgaat tcagattatt attgtaaatg gttagttcgt cctgccaggc tgaaagccgg
     2041 ttctgaagag ttagtaatat gttttttgtt gcaattaaaa ttttaagact cgtctgtccc
     2101 ctgaatccct ttaaattgat ctttgctacg gaaattattg aatctgtttc cggtaatcca
     2161 ttgctgatcg atgttgtatc aaatccgcgc ctcagtgttg aattaatttt atttaatgct
     2221 aatgtatatt cttcaatttt attaagctga taggatatca gggtgtctgt agctttaaaa
     2281 tactttttta cagaatctgt attaaaggtt tccagacgga tggaatcaaa gtttataagc
     2341 gatgtatcaa ttttttcatg taccggcaaa gaatcctgta tctgaaacgt aaaagcgctg
     2401 aacaggtata taaatatcaa acatacaaca ataatccctc ctctgctgaa ctgttttgat
     2461 ccgccggcct ttttaattag aatcataaat aaaattcttt tgatacaaaa atatttgagt
     2521 aaatctaata ttcaaaatta accttttctg tacattaaat ttaaattatt ttgctcaaat
     2581 attgaatgtt caaaattagc tgtaatttat ttatagtcca agtaaataat tttttgctat
     2641 ttgaaaccgg tgctgtccaa aacattttaa attttaaata aatagtcctg aaattattga
     2701 aaaatacaat tcattgatct gattgaaaac tatccctcat ttgaattcct ctattagcta
     2761 atttatcaca ggcggcgatt tttttggttc tttggagcct gcccctttgt gcttccggat
     2821 tgtctcaaac ttttttcttt tgtgtcttgg tgtcttggtg gtagtgtctt tgtggtaaag
     2881 ctaaatcaga tttataagcg gaaactttcc ggattcttaa ttgattttat ttctcttctg
     2941 tacagctcct cagccatttt aagttttatt tttaagtcat caatatctgc tatacttaaa
     3001 agtgactcaa tttcttttac aagatattcc ggaagctcgt catacaaata tctcaattcg
     3061 aaattataat gtaccggatt atatttgacc ctaagggatc tgatcagagg ttttaatgtg
     3121 agatcaaaat aataggcaag tgcatcaatg taatgtcccc ggattatttc cttttcagtc
     3181 tgataaagaa aaaattcaaa aacacttttg tatttatatt caaactcatc tatttttttc
     3241 tgtaataatt ctttgtcata agtctgatct ttcatataat catatttatc ataatgaatt
     3301 acaagatcac cgtgaatatg cttttccatc tccttcatgg gatttgatct ctcgacaatg
     3361 aacagatcaa caatggaaaa tttatcagta tctttcagct tatagaattt atgctgtctg
     3421 tcattaatgc cggaattttc atggacgttg tagaccgaat tgatcccggt aagttcctcc
     3481 agaaaatcct caaacttctt tactgtttca ccggcagtcc catcttctgt atccaccatc
     3541 agatcaatat ccgaaagttc atcataacgg ttaaatgcct ttgctccgcc ttcagcaatc
     3601 aaattgacct gtggatatga tcttaattct tttaagatct cagtaagtat ttcttctctg
     3661 tgcatgtatt aaaatttgaa ttagaaaatt attaatattc atcgtatttt attttaaaat
     3721 ttattttcaa cttttttgat aatatctgaa atcaccgttt ttcctgttag ggattttaaa
     3781 tttataagca aatgatgcat ttcctcccgg atcatcctgt catcttttcc ataatcccat
     3841 atcggtaata acgtagcagt aaagatcttt tcaaatgtta actcttttaa aatgataccg
     3901 gctttatcag attcagtcct gatgtaagtt tccggataat ttgaagctct gtataaatat
     3961 aactcaaaca gcgctctcag actgctgacc gcagtagcag gatcattaat accgggactc
     4021 agtgctttta ccgccacttc tgtcagctgt ctgcatccga aataaaaatt gttcttgatg
     4081 gattcactct catgaatgta gagagaattt attaattccg tttttaaagt ttcaggaagt
     4141 tccttatttg atttgattac aggcatccct ttcaaaatga atgtgccggg tgtgtgaatt
     4201 aaataaagca tgcattcatt tttttcacag attgagataa aggaattttt attaaatcct
     4261 tcatatatcc ccgggcattg agaatttata atatgatcac ttactgagtg aaatatttcc
     4321 gccggctctg tatttgaaat acagctttgt tccaatgctt cgcgggtttc cttatatatc
     4381 ctgcgaatga tcacttcgta ttttacggat tgtgtgatgt agtgaagaaa atatatgaaa
     4441 agaaacacat ccaggacagt taggagcatt agtaaatatg tgctcagtac agggatctgc
     4501 agtccggaat caatatctct gattgaactt aataaaaaga gtgcaaatac aattgttcct
     4561 acatagatcc cgagcaccat ttgctggtac cggctgccga taagattatc cagtatcctg
     4621 ttactcatct gtgaagccgt ctgattaagc actatcatca ccatcgaaaa actaaataca
     4681 gtaagtgaaa ttattcctgc tgctatggaa gaaataatgc ttcgggctgt agatggatcc
     4741 tgcagcttca gccatttcag atttgatctg atatgctgcc cgctttccga aaaatcaaat
     4801 gcaatactga aatatgaaag caataaaaat ccaagagcaa taagcgcagg ataaaatgct
     4861 atacttttta ttagtttatt gaaattttct ctgatcatat tagacaaatt aactgataat
     4921 tagataaact atattttgtg aatcgattta aaattgtatt cattaaaata ttgatctcaa
     4981 aaatttaaaa tgtatctaaa gattatattt cttaaaacta attaacgtaa ttcaaatata
     5041 caaataaaat taagcgaaaa atattaatag aaacttttaa tcagtactgt ccaaaacatt
     5101 ttaaatttta aataaatagt tctaaaatta ttgaaaaata caatttatta atttaaatga
     5161 aaactat
//
LOCUS       DAORVQ010000224         5113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_11355_length_5113_cov_7.099644, whole genome shotgun sequence.
ACCESSION   DAORVQ010000224 DAORVQ010000000
VERSION     DAORVQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11355_length_5113_cov_7.099644"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..686)
                     /locus_tag="PLX80_13065"
     CDS             complement(78..686)
                     /locus_tag="PLX80_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21765.1"
                     /translation="MSDFILEAVKCKNCGSGLAVEVNDNIVYCTSCGSGFEEIDGEMK
                     NINIDFASAAIRSEGELIYKPFWLLHTTITVHERKATGNFIKNLFGGSNDFTNGDILF
                     YIPAFYCNLENLKNLSTQFTMKNPVASPQKYNSKLTGFAYGKDDAKKLAEFILISLEA
                     EKSDVITSFNYEMKVNSYSILGIPFYDLKNGRYKDAVLGVEF"
     gene            complement(726..1877)
                     /locus_tag="PLX80_13070"
     CDS             complement(726..1877)
                     /locus_tag="PLX80_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21766.1"
                     /translation="MSEETEIIDIPKPPVSVKKEKIIRGITCPSCSGELDLREGLRSF
                     NCKYCGTLLVVRGESGTIKYYVPKKLKREDAVAKALGWLGKGISKARKLRTDAKIEDA
                     FLAYIPYWRVRADVVGWVFGQEKRTSTSNGRTTTTYIDTEKKIQKSFERTYSACDVAE
                     LGVKKINLTGDTIHPVEFETLQQDGMVFNIIASEKEAFEFAKENFSGEARNAINLYNI
                     TFEHFDLVRESIDIVYYPLWVVRYSFAGRTYQVVVDGEDGSICYGKAPGANLFRAVMG
                     ILGTGIGMYMMTFLGVFSMLEFDEKFPFIAFFIIFFIGMAVTRWGYKKFRYGGEVEEG
                     TGLEVDSQTSVNSIVTDLKSNSAFNATKNIAASVVIGSVVSALLKSSRR"
     gene            complement(1896..2717)
                     /locus_tag="PLX80_13075"
     CDS             complement(1896..2717)
                     /locus_tag="PLX80_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21767.1"
                     /translation="MAEVSSSKIKCPQCGGENNIKADEKFLECEYCGSSIYIDKRKVV
                     NHFVVNSNFTKENAEGNLRRWMAGNFHVKDLDKLSQISQVNFYYFPLWYFKTKDSQGD
                     KIYLQPAHSTPVSEIKNLQIPAGNLKPYHKNDFKDQVFVEADVLYESAKSWLSQSGVD
                     PETISESNLIHVPFYHFYYTFKGQQYSALVEASSGMVYANIWPAKSEMPFRILFGAAI
                     AVFAIASIISFAAAFMLSSDLSAETLFMGEFLKVFIYILASIPLIAIAFWIVKKV"
     gene            complement(2722..3711)
                     /locus_tag="PLX80_13080"
     CDS             complement(2722..3711)
                     /locus_tag="PLX80_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQY21768.1"
                     /translation="MAFIDIVQFFDETGKTLVHREPQDGSSAFRLGTQLIVQDSQSAV
                     FYRDGKALDVFNAGRHTLTTENIPFLTKLISIPFGGTSPFQAQIYFISMKKFIDLKWG
                     TKSPINFKDSELTYVQLRASGKFSMRITDPRLFMMEIVSTQGLYTTNDIEDYLRDAIV
                     SRLNSVLGKNLKTVFELGQSYQQIESGVKAEVADFFNSMGIELTDLILIGIVPPDEVQ
                     EKINERSSMGAVGNLDNYMKFKTAGAIEKAAENESGGGLAGAGVGLGAGMTMANMMGN
                     QMANSNQQSGQQEKKMTSDEIMSTIEKLGKMKTDGLITQDEFDAKKKDLLSKL"
     gene            complement(3786..4313)
                     /locus_tag="PLX80_13085"
     CDS             complement(3786..4313)
                     /locus_tag="PLX80_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21769.1"
                     /translation="MNDLQSKLEQVKSNESWLEKIKRYIPGYDGYVNRDNSRELDTIL
                     RNKLAVLLDENKTKLKNTVSVLSENGNLLSVTGLDKIDKKNETVIAKLRSAARGYSGA
                     FDVVKIKEDKLNQLYQFDGSLLENAEAVCNKFADLESKAKAKEDLAAHVSEISDTLDG
                     FLNKFDEREDILRQL"
     gene            4405..4977
                     /locus_tag="PLX80_13090"
     CDS             4405..4977
                     /locus_tag="PLX80_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21770.1"
                     /translation="MNRKIILPITFLLLTQIIFQINSGNCFSENTNLNYYKQTSLFFL
                     DTNQNNSLTISDAVNFVKEISGGEVISAERRFKKSDPVWEVILISEEGSFVKMEISFY
                     ERTVYMMNSDEGPFTYDIKPATDLISLSEAIKIADSRSSQKVLKWKFLKVKDKWEYNF
                     WLFTKSGKAQLRLDAETGETITGKKTKKNK"
BASE COUNT         1487 a         1051 c          859 g         1716 t
ORIGIN      
        1 agacggaaaa agtaaagata ggattttctt agtgtcttag tgccttggtg gtaaacacgg
       61 aacttatccg gagagaatca gaactcaacc ccaagcaccg catccttata ccgcccgttt
      121 ttcagatcat aaaaagggat tcctaaaatt gagtaagaat ttactttcat ctcataatta
      181 aaacttgtta tcacgtcact tttttctgct tccagtgaga tcaaaataaa ttcggcaagt
      241 ttttttgcat catcttttcc gtatgcaaat cccgtaagtt tggaattata tttctgaggt
      301 gacgctaccg gatttttcat agtgaactga gtgcttagat tttttaagtt ctccaagtta
      361 cagtaaaatg caggtatata aaaaagaata tcaccgtttg taaaatcatt actgccgccg
      421 aataaattct tgatgaaatt tccggtagct tttctttcat gaactgtaat agttgtatgc
      481 agaagccaga atggcttata gatcagctca ccctctgatc tgattgcagc cgacgcaaaa
      541 tctatattaa tatttttcat ttctccgtcg atctcttcaa atccgcttcc gcagcttgta
      601 cagtaaacta tgttatcatt cacttcgact gcaagtccgc tcccgcagtt cttacatttc
      661 acagcttcta aaatgaaatc actcaaaata ttttataaaa ttaattcctg aattaagttt
      721 gctctctatc tccttgaaga tttcagtaat gcacttacta cactgccgat cacaactgat
      781 gcagcaatat tcttagtagc attaaaagct gaattgctct tgagatctgt aacgatggaa
      841 tttactgatg tctgactgtc gacttccaga cccgtccctt cttcgacttc accgccgtat
      901 ctgaattttt tatatcccca tcttgtgaca gccataccaa taaaaaagat aataaagaat
      961 gcaatgaaag gaaatttttc atcaaattcc agcattgaaa atactccaag aaacgtcatc
     1021 atatacattc cgatacctgt tcccaaaatt cccattacag cacgaaataa attcgcaccg
     1081 ggagcttttc cgtaacagat acttccgtct tcaccatcga ctaccacctg atatgtcctc
     1141 ccggcaaaac tgtatctcac cacccatagc ggataatata ctatgtcaat actttccctt
     1201 accagatcga aatgttcaaa agttatattg tataaattta ttgcgttcct tgcctctcct
     1261 gagaaattct cttttgcaaa ttcaaaagct tccttttctg aagcgattat attgaacacc
     1321 attccgtcct gctgaagtgt ttcaaactca acaggatgaa ttgtatctcc tgtcagattg
     1381 atctttttca ctcccagctc agctacatca caggcagaat atgttctctc aaatgacttc
     1441 tgaatttttt tctcggtgtc gatataagta gtagtagtcc ttccatttga agtgcttgtc
     1501 cttttttcct gcccgaatac ccatccgact acgtcagctc tcacacgcca gtaaggaatg
     1561 tatgcaagaa atgcatcttc gattttagca tcagtcctta actttctcgc tttggaaatt
     1621 ccttttccca gccagccaag agcttttgct acagcatctt cacgtttcag ttttttagga
     1681 acgtaatatt ttattgtacc gctttcacct ctgaccacca gcaaggtccc gcagtattta
     1741 cagttaaaag atcttaaccc ttctctcagg tcaagctccc cgctgcatga aggacaagtg
     1801 atgccgcgga tgatcttttc tttttttaca ctgacaggtg gtttcggaat atcaataatt
     1861 tcagtttctt cagacatgtt ttttgataaa ataagttata ctttttttac tatccagaat
     1921 gcaatggcaa tcaatgggat cgaagcaagt atgtatataa atactttcag aaattctccc
     1981 atgaataatg tctcagcaga taaatccgaa gagagcataa acgcagctgc aaaagaaatg
     2041 atactggcta ttgcaaatac agcgatcgca gctccgaata aaattctgaa aggcatttcg
     2101 ctttttgcag gccagatgtt tgcatatacc attcccgatg aagcctctac taaagccgaa
     2161 tactgctgac ctttgaatgt ataataaaaa tgataaaatg gtacgtgaat caaatttgat
     2221 tcggaaatgg tttccggatc tacacctgac tgtgaaagcc agcttttcgc agactcatac
     2281 aatacatccg cttcgacgaa tacctgatcc ttgaaatcat ttttgtgata gggttttaga
     2341 tttcccgcgg gtatctgcag attttttatt tctgataccg gtgtcgaatg cgcaggctgc
     2401 agatagatct tatctccctg cgaatccttt gtcttgaaat accataaggg aaagtaatag
     2461 aaatttacct gcgatatctg cgagagttta tcaagatctt tgacgtgaaa atttccggcc
     2521 atccatcttc gcagatttcc ttctgcattc tcttttgtaa aatttgaatt tacgacaaaa
     2581 tgatttacaa cttttctttt gtctatgtaa attgatgacc cgcagtattc acattcgagg
     2641 aatttttcat cggcttttat attattttca ccgccgcact gcggacactt tattttagag
     2701 gaactgactt cagccattta attataattt agaaagcagg tcttttttct ttgcatcaaa
     2761 ttcatcctgt gtgatcagac cgtctgtttt catttttcca agtttttcaa tagttgacat
     2821 tatttcatcc gatgtcattt ttttttcctg ctgtcctgac tgctgattac tgtttgccat
     2881 ttgattaccc atcatattcg ccattgtcat tcctgctccc aatcctacac ctgctccggc
     2941 taatccgcca ccggactcat tctctgcagc tttttctatt gcaccggctg ttttgaattt
     3001 catgtaattg tctaaatttc caactgctcc catcgaactt ctttcattta tcttttcctg
     3061 tacttcgtcc ggcgggacga tcccgataag gataaggtcg gttagttcaa ttcccattga
     3121 attaaagaaa tcagctactt cagcttttac accggattcg atctgctgat aactctgtcc
     3181 aagttcaaat acagtcttaa gattttttcc aagaactgaa ttaagtcttg atacaattgc
     3241 atcacgcaga taatcttcaa tgtcattggt tgtatataga ccctgagtag atacaatttc
     3301 catcatgaac agtcttggat cggtgatcct cattgagaat tttccggatg ccctgagctg
     3361 aacgtatgta agctctgagt ctttaaagtt gatcggtgat tttgtacccc atttcagatc
     3421 aatgaatttc ttcatagaaa tgaaatagat ctgcgcctgg aaaggtgatg tgccgccaaa
     3481 cggaatgctg atcagttttg taagaaatgg aatattctct gtagtcagag tatgtctgcc
     3541 tgcattaaat acatcaagcg cttttccgtc tctgtaaaaa actgcgcttt gtgaatcctg
     3601 gactatcagc tgcgtgccta atctgaatgc ggatgaaccg tcctgcggct ctctgtgcac
     3661 cagagttttt cctgtttcgt caaaaaattg tactatgtct atgaatgcca tgattaatat
     3721 ttatttgaat taataattgt ccgcagtgcg gagtttgtta taattttaaa tttgtttaaa
     3781 gctgcttaaa gttgtctgag tatgtcttct ctttcgtcga atttatttaa aaatccgtcg
     3841 agagtatctg aaatttcaga tacatgcgct gcaagatctt cctttgcttt tgcttttgat
     3901 tcaagatctg caaatttatt acagactgct tcagcgtttt caagcaagct tccgtcaaac
     3961 tgataaagct gattcagctt atcttctttt atttttacaa cgtcaaatgc gccgctgtaa
     4021 cctctcgcag ccgaacgcag ctttgcaatt acagtctcat tttttttatc gatcttatcc
     4081 agtccggtca ctgacaataa atttccgttt tcagaaagta ctgaaactgt attttttaat
     4141 ttggttttat tttcatcaag taaaacggcg agtttgttac ggagtatagt atcaagctct
     4201 ctcgagttat ccctgttgac ataaccgtca tatccgggaa tgtacctttt gatcttttcc
     4261 agccatgact cgtttgactt tacctgctca agttttgatt gtaggtcatt catttatttg
     4321 tgtattttaa ttttataaaa aatagattta attgctttgg aagaatttca gtaacattaa
     4381 attaattaaa ggagataaaa aaacatgaac agaaaaatta ttttaccgat tacattttta
     4441 ttactaactc agattatttt tcaaattaat tcaggaaatt gcttttcaga aaatactaat
     4501 ctgaattatt ataagcaaac ctctttattt tttcttgaca caaaccaaaa taattcactt
     4561 actatttcag atgcagtaaa ttttgtaaaa gaaatatccg gaggtgaggt aatttctgca
     4621 gaacgcagat tcaaaaagtc cgatccggta tgggaggtaa tactaatatc agaagaggga
     4681 agtttcgtaa agatggaaat ttctttttat gaaaggacag tatatatgat gaattctgac
     4741 gaaggtcctt tcacttacga tataaaacct gcaactgatc taatttctct gtcagaagca
     4801 ataaagatcg cagattcccg atcatctcaa aaagtcctga aatggaaatt cctcaaagta
     4861 aaagataaat gggaatacaa cttctggctt ttcacaaagt ccggcaaagc ccagctcaga
     4921 ctggacgcag aaacgggaga gaccattaca gggaaaaaga ctaaaaagaa taagtagact
     4981 tattttttta aaacgggtta cggatttgga gatacggatt tcgaattacg tgtcacgacc
     5041 tattttctac accgcctgtc agtagttaat agttaatagt taatagttaa tagttaatag
     5101 ttaatagtta ata
//
LOCUS       DAORVQ010000225         5112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_11361_length_5112_cov_6.295432, whole genome shotgun sequence.
ACCESSION   DAORVQ010000225 DAORVQ010000000
VERSION     DAORVQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11361_length_5112_cov_6.295432"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..3183
                     /locus_tag="PLX80_13095"
     CDS             34..3183
                     /locus_tag="PLX80_13095"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HQY21771.1"
                     /translation="MYKTKLFLFIVALLICVTGNSFAAHLNFTSYLTGEQESPSVITN
                     AKGTGTYTLTAGGLRYTITVNGLSGPIVAAHFHIGTAKVNGPVVYNITGSFIGNTAAG
                     TIAGPLPDSIIAFLMTGKIYVNVHTAANPGGEIRSQVNISAGTNLIARLDGLQENPSL
                     MNSAKGTASITLGSVGSVGLAYNISANGLSGPITGAHFHYGKIGENGPVVHNITSVFN
                     GENAVGVWRTSAAMPLNDTLIMALLTNKLYINLHTAANPSGEIRGQVVLAAGFGAYAN
                     LNGASENPPVITSSTGTSELTFTDYGLVYNITVDGLSGPITGAHFHDADSGTNGPVVF
                     NITSSFNGNTATGLWKASGAAGDLNPTLMKRLFANGLYLNVHTGANPGGEIRGQVMMK
                     PGSGIGAFFTGLQEVPAPVITTASGTASLFTNSMGLRYFITVTGLSGPITGAHFHLGS
                     TKEGGPVVKDITSTFTGNTANGTWLIAEATPFNDSLRQALVNGRIYLNIHTAANPSGE
                     IRGQVFLTAGAGMTANLDGNQQNPAVVTSSKGTGQFTMTRGGLGFNFSFNGLSGPVTA
                     MHFHYGGIGINGPVVYDIGPDVNGNNASGYWRSAASVDSLFNALMTGRIYVNVHTAAN
                     PGGEIRSQVNLSEGLGITVQLTGSQENPPVTTNGRGTGSATITDPGIVFFNTFDMLSS
                     PLQAAHFHNAPMGMNGPVVKDLMSYIKGNSILGIWKRNDVMMPISNTMIAEAYNQNIY
                     INLHTSNFPGGEIRGQLRGGAIPFIPYQIISTLMITPQNSTGNVQTPSCLSAELKDIM
                     GNPVRNTNIDFIIKGVNPGMGMAMTDTSGIAQFCYTGLNLGKDTIIGMSAGVMDTAII
                     IWDEPLPVELTSFAYSVIRNDVSLNWTTASEINNSGFDIERNDINSVEWTKISNIAGT
                     GNSSTPVNYSYSDKNVATGTYNYRLKQIDFNGNFEYFNLNSEVLVGIPAKYNLSQNYP
                     NPFNPSTKIDFELPVDDNVSITLIDNSGRLVSTITSGFRTAGYYTISFNASNLASGIY
                     YYRLQTNSYNKVLKMTLIK"
     gene            complement(3434..4192)
                     /locus_tag="PLX80_13100"
     CDS             complement(3434..4192)
                     /locus_tag="PLX80_13100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21772.1"
                     /translation="MLFRKVLPGSASRLGIVDHNESYGRHVLENCIKSLNIKKCVDLG
                     CGSGDDLSIVSKYNPGAELIGVDYGLWNEDVLKKKGIQSVSVNIETDRLPFDDGSVDF
                     VIANQILEHTKEVFWINHEIFRILKTGGLLFMGTPNLLSLHNRILMMFGNHPTQHKLT
                     SAHVRPFSKKDTKLFYNQLAGSLISFEGFWGSQFYPFPKSTARFLSRLFPSSAFSIFF
                     LIRKTNEYNSEFINWLDVAKLETNFYNGKNINNI"
     gene            complement(4463..>5112)
                     /locus_tag="PLX80_13105"
     CDS             complement(4463..>5112)
                     /locus_tag="PLX80_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21773.1"
                     /translation="TAGIWKLVINDLTGPGIGKLYSWGVQFNNSSLSASALCLKYFTE
                     GFYRSVDSTVLDTIKVKLREDVSPYLEVGIKGETPDEDNIIRYNFPEADFISDYYIQV
                     NHRNSIEIWSDTAYSFNTLTGNMIYDFTVSPESAYGDNLVQVENSPDIFAAYGADVDQ
                     NGVVDLTDNSLIDNDVLTFAFGYVVSDVNGDETVDLSDQSLADNNSFMFVTKVTP"
BASE COUNT         1674 a          933 c          925 g         1580 t
ORIGIN      
        1 attttttaat taatatattc gaaaggaaat agtatgtaca aaactaaact ttttttattt
       61 atcgttgcac ttttaatttg tgtgacagga aattcatttg cagcgcattt gaatttcaca
      121 tcttacctta caggtgagca ggaatcacct tcagtcataa ctaatgccaa aggaacagga
      181 acttatactt tgactgcagg cggactgaga tataccatta ctgtgaacgg acttagcgga
      241 ccgatagtag cagctcattt tcatatcggt acagcaaaag taaacggacc tgttgtttac
      301 aatatcacag gttcttttat tggaaacact gcagcaggaa ctatagccgg tccattaccg
      361 gattcaataa ttgctttctt aatgaccggg aaaatttatg ttaacgttca tactgcagca
      421 aatccgggcg gtgagatcag aagtcaggta aatattagtg caggtactaa ccttattgca
      481 agattagacg gacttcagga aaatccatct cttatgaatt ccgcaaaagg aactgcctca
      541 attactcttg gcagcgttgg aagtgttggt cttgcttaca acatttcagc aaacggacta
      601 agcggtccta tcacaggtgc acattttcat tacggaaaga tcggagagaa tggaccggta
      661 gttcataata taacttctgt attcaatggt gaaaatgctg tcggagtatg gagaacatca
      721 gcagcaatgc ctttgaatga tactctgatc atggcattat tgacaaacaa attatatata
      781 aatttacata cagcagctaa tccttcaggt gagatcagag gacaggttgt ccttgctgcc
      841 ggattcggtg catatgcaaa tcttaacgga gcaagcgaaa atccacctgt gatcacttca
      901 tcaaccggaa catcagagct gacttttaca gattatggtc tggtatataa tattacagtt
      961 gacggattaa gcggtcctat caccggtgca cattttcatg atgcagattc aggtacaaac
     1021 ggtcctgtag tatttaatat cacatcaagt tttaacggca atactgcaac tggattatgg
     1081 aaagcatcag gcgctgcagg tgatcttaat ccaactctta tgaaaagatt atttgcaaac
     1141 ggattatatc ttaatgttca cacaggtgct aatccgggcg gtgagattag aggacaggtg
     1201 atgatgaaac cgggttcagg aatcggagcg ttctttactg gtctgcagga agttcctgct
     1261 ccggtaatta ccacagcttc aggaactgct tcattattta caaactcaat gggtttaaga
     1321 tattttataa cagtaacagg tcttagcgga ccaatcacgg gagcacattt tcatttaggt
     1381 tctactaaag aaggcggacc cgttgtaaaa gatattactt caacatttac aggcaataca
     1441 gctaacggaa catggcttat tgctgaagca actcctttta atgactcact cagacaggca
     1501 cttgtgaatg gaagaattta tctcaatatt catactgcag caaatccttc aggtgagatc
     1561 agaggtcagg tattcctgac agccggtgca ggaatgacag caaaccttga cggtaatcag
     1621 caaaaccctg cagttgtaac ttcttctaaa ggaacaggtc agttcacaat gacaagaggt
     1681 ggtcttggat ttaatttttc attcaacgga ttaagcggtc ccgttacagc tatgcatttc
     1741 cattatggtg gtataggaat aaacggaccg gttgtttatg atatcggacc tgatgtaaat
     1801 ggtaataacg cttcaggtta ctggagaagc gcagcaagtg tagattcatt atttaatgca
     1861 cttatgacag gaagaattta tgtaaacgtt cataccgctg caaatccagg cggagagatc
     1921 agatcacagg taaatttaag tgaaggatta ggaataactg ttcagcttac aggatctcag
     1981 gaaaatccgc ctgtgactac aaacggaaga ggaacaggtt ctgctacgat cacagatccg
     2041 ggaattgtat tttttaatac ttttgatatg ctgagttcac cactgcaagc tgctcatttc
     2101 cacaatgctc caatgggaat gaacggtcct gtagtaaaag atctgatgtc ttatataaaa
     2161 ggcaacagta ttcttggaat atggaaaaga aatgatgtaa tgatgccgat ctcaaatact
     2221 atgatagcag aagcttacaa tcagaatatt tatatcaatc ttcatacttc taattttccg
     2281 ggcggtgaga tcagaggaca attaagaggc ggagctattc catttatacc ttatcagatc
     2341 atttcaactc tgatgataac tccgcaaaac agcactggaa atgttcagac accttcttgt
     2401 ctaagtgcag agctaaaaga cataatggga aatcctgtaa gaaatactaa tatagacttt
     2461 attattaaag gtgtaaatcc gggaatggga atggcaatga ctgatacttc aggaattgct
     2521 cagttctgct acacaggtct taaccttgga aaagatacta tcatcggaat gtcagcaggc
     2581 gtaatggata cagctataat aatctgggat gagccgcttc ctgtagagtt aacttcattt
     2641 gcatattccg ttatcagaaa tgatgtttct cttaactgga ctacagcatc agagataaac
     2701 aacagcggat ttgatattga aagaaatgat attaattcag ttgaatggac aaagatctct
     2761 aatattgcag ggacaggaaa ttcaagtaca cctgtgaatt attcttattc agataaaaat
     2821 gttgctaccg gcacatataa ttacagatta aagcagatcg attttaatgg taactttgaa
     2881 tatttcaatc tgaacagtga agtacttgta ggtattcctg ctaagtataa cctgtctcaa
     2941 aattacccga atccgttcaa tccatctaca aagatagatt ttgaattacc tgttgatgat
     3001 aatgtatcaa ttacattaat tgacaacagc ggtagattgg tttctacaat taccagcgga
     3061 ttcagaactg caggatatta cacaataagt ttcaatgctt ctaacttagc aagtggaatt
     3121 tattattaca gattacaaac taacagttat aataaagtat taaaaatgac attaataaaa
     3181 taatttaagt accccaaagc ttaaatattt ttattgaaaa gagcgggtcg tcgggacccg
     3241 cttttttttt atacatttcc aaaaaaaata taaaaaaatt tattgaagaa ggtatatcaa
     3301 aaaatgatta tgagtttaag tcattttcat tttatttcaa attcgatctt atgatttaaa
     3361 ataaaattca gataaaatta taatgttttg attttatttt acaggaagat tttacagaag
     3421 ttaaaacgaa attctaaata ttattaatat ttttcccatt gtaaaaattt gtttccagct
     3481 ttgcaacatc cagccagttt ataaattcac tattgtattc gttcgtttta cggatcaaaa
     3541 aaaatattga gaatgctgag gaaggaaaca gcctggataa aaaacgtgcg gtagattttg
     3601 gaaaaggata gaactgcgaa ccccagaatc cttcaaacga aatcaatgat cctgctaact
     3661 gattataaaa aagtttagta tctttttttg agaatggtct gacatgtgct gaagttaatt
     3721 tatgctgcgt tggatgattt ccaaacatca ttaatatcct gttatgcaga gacaaaagat
     3781 ttggcgtacc cataaaaagt aacccgcctg ttttcaatat tctgaatatt tcatgattga
     3841 tccaaaatac ttctttagta tgctcgagga tttgattagc aattacgaaa tcaacagatc
     3901 catcatcaaa aggaagcctg tcggtttcaa tatttactga cacagattgt attccttttt
     3961 ttttcaaaac atcctcattc cacaaaccat aatccactcc aatcagctca gctcccggat
     4021 tgtatttcga tacaatcgaa aggtcatccc cgctcccgca tcctaagtca acacattttt
     4081 ttatgttaag agatttaatg caattctcga gtacatgtct tccataagat tcattgtgat
     4141 ctacaattcc cagcctgctt gctgatccgg gcaatacttt tctaaacagc attaatgatt
     4201 atttcttagt atttgaaaat gaatttttta aatctgataa cgataacaat ttgttataga
     4261 aaataaaact tacttttatt gacagtttat ttataaaatt ttccggatct gaattttatt
     4321 aaaagaaagt ttgcgttggt cgttggtcat tggtcatgtt cgctttgttc actaatacca
     4381 gcaacgtctc aaatttcaaa ttctaaaaaa agctgccctg ataaaattcc tgattcctaa
     4441 ttctcaattt tcaattgctt ctttaaggag ttactttcgt cacaaacata aacgaattat
     4501 tatctgccag actttggtca gaaagatcta cagtctcatc accatttaca tctgagacta
     4561 catatccaaa tgcaaatgtc aatacgtcat tatcaataag tgaattatct gtcagatcga
     4621 caacaccatt ctgatccaca tcagctccat acgctgcaaa aatgtcagga gaattttcta
     4681 cctgtacaag attatcacca tatgcagatt cgggactaac tgtaaaatcg tatatcatat
     4741 tccctgtcag tgtattaaaa gaatatgcag tatcactcca aatctctatc gaattcctgt
     4801 ggttaacctg tatgtaataa tctgaaataa aatctgcttc agggaaatta tatcttataa
     4861 tattatcttc atccggtgtc tctcctttta ttccgacttc aagataagga gaaacatctt
     4921 ctctcagctt tacttttatt gtatctaata cggttgaatc aactgacctg taaaatcctt
     4981 ctgtgaaata tttcaaacaa agagctgaag ctgacaatga agagttatta aactgaactc
     5041 cccaggagta aagttttcct atacccgggc ctgtcagatc gttgattacc agcttccata
     5101 tacctgctgt ca
//
LOCUS       DAORVQ010000226         5095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_11426_length_5095_cov_6.683135, whole genome shotgun sequence.
ACCESSION   DAORVQ010000226 DAORVQ010000000
VERSION     DAORVQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11426_length_5095_cov_6.683135"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PLX80_13110"
     CDS             <1..1362
                     /locus_tag="PLX80_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21774.1"
                     /translation="GNQTRRDLVVIKYSPAGANLWTRRIADTVNNGYMSGRDIQMDEF
                     SNVIVGGSGIHKYDSNGNLLWSNPDVGCYVFDFDSAGNVYTISGNTYYITRKLNLSGV
                     ILWERIYPGYQVIDPNRPYDMCIDKENNVLVTGKCRQATVTLNDYATVKYSNTGNLIW
                     ERRYNGGNEDIAYAITSDDSNNVYVTGWNKNISTDILTIKYSPEGDTVWKAVYDGGGG
                     DVGYDIEVDSLGNVYVGGVTNSSSYITIKYDVNGNLLWSRVQISQQIPYFPVLKLDKN
                     RNVYMSFVSFRPGNFSNYAVVKYNNDGVQQWMAEYYNTGFSHIYDLTLDTQANIYVTG
                     ASGGGHGYSIATVKFVQTPTQINQSTNNIIPDSYWLGQNFPNPFNPKTVIQYQIPVKG
                     LVLLKVFDVLGNEVAQLLNGNQEAGSYEAEFDGSGFASGIYFYSLYLNESLFDTKRMV
                     LLK"
     gene            1720..2397
                     /locus_tag="PLX80_13115"
     CDS             1720..2397
                     /locus_tag="PLX80_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21775.1"
                     /translation="MKKSSSIFIIIFIMCSSGIFAQVNQEWVATYSGNGLGNNVPKKN
                     LVDKENNIYVVGRSYGTGDDFITLKYNPSGNLLWEKRYNGTGNGDDGISAAILDDSNN
                     IYVTGYSQEGAALGGYNWITIKYYSNGDTAWKRSFNWIANSTDEPLAITIDRFNNIYV
                     GGYGRSGQPIDDDFITIKYNSNGDLLWLDSYDSNVFGPDRVMSIAIDDSSNVYPTGYS
                     AFSGGNE"
     gene            2587..3348
                     /locus_tag="PLX80_13120"
     CDS             2587..3348
                     /locus_tag="PLX80_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21776.1"
                     /translation="MWKRIYKGDGNLNFCFALTTDDSGNVYAAGRSTNTGTGEDYVTI
                     KYYSDGDTAWIRNYDGGYDNFDEARSVVVDNYQNVYVTGLTSSSTGRPSYTTIKYNKF
                     GDISWIKNFSGSYLTDVATSLKIDINYNVIVTGYSQTSINFASIATIKYSQITGFVDK
                     TNLFNLDFKLYQNYPNPFNPSTTIKFTLISESNVILKIYNALGKEIETVINIKKPKGT
                     HVVIFNGKNFTTGIYFYSLFINGNLIDTKKMILIK"
     gene            3353..5017
                     /locus_tag="PLX80_13125"
     CDS             3353..5017
                     /locus_tag="PLX80_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21777.1"
                     /translation="MKKIKFKNTFLIVVFLFNYSFLSDISAQVNQEWVATYSGIGLGS
                     NAPKKNLVDNENNIYVVGRSYGTGDDFITLKYNPSGNLLWEKRYNGIGNGDDGISAAV
                     LDDSNNIYVTGYSQEGAALGGYNWITIKYYSNGDTAWKRSFNWTANSTDEPFAMTIDK
                     SSNIYISGYGRTAQPFDDDFITLKYSSNGDSEWTKVYASKSLNPDRSNSITVDDSSNV
                     YASGYGGTPDNNEIITIKYNSSGVEKWIKRFPTNYADLLRHTISANDKNNNIIVNGYY
                     YFGDHYAFNTLKYNSQGDLLWNRIYKGDGNLNFCHALTTDDSGNVYAAGRSTNTGTGA
                     DFVTIKYYSIGDTAWIRKYDGGFNQFDEAYSIAVDDYQNVYVTGMSDSTDGLSDYLTM
                     KYDSDGNINWIKKYNGYFLVDRSFCLSIDNNYNIIISGFAQKNINDAWITSIKYSQFT
                     GVSGNDIDYNFNSVLFQNYPNPFNPATNIKYQLYSTGIVSIKIYDIIGKEIISLINKL
                     QNSGTHEIKFDINNPENRGNLPSGIYFYSLLIDGKIIDTKRMILIK"
BASE COUNT         1877 a          681 c          958 g         1579 t
ORIGIN      
        1 gggaatcaga ctagaagaga tttagtagta ataaaatatt ctccggcagg agcaaatctt
       61 tggacaagaa gaattgctga tactgtaaac aatggttata tgtccggtcg agatattcaa
      121 atggatgaat ttagtaatgt tattgttggc ggatcaggta ttcataaata tgattcaaat
      181 ggaaatttat tgtggagtaa tcctgatgtt ggttgttatg tttttgattt tgatagcgcg
      241 ggaaatgtat atactatatc aggcaatacc tattatataa caagaaaatt aaatttgagc
      301 ggagttatat tatgggaacg catatatccc ggttatcagg ttatagatcc aaatcgccca
      361 tatgatatgt gcattgataa agaaaataat gtgttggtta caggaaaatg cagacaggca
      421 acagtaactt tgaatgatta tgccacagta aaatattcaa atacaggcaa tctgatatgg
      481 gagcgaagat acaacggcgg aaatgaagat attgcctatg caattaccag cgacgactca
      541 aacaatgttt atgtaaccgg atggaacaaa aacatcagca cggatatact gacaataaaa
      601 tattcacctg aaggcgatac agtatggaaa gcggtttatg acggcggcgg tggtgatgtg
      661 ggatatgata tagaggttga ctcacttggt aatgtgtacg taggaggcgt gacaaattca
      721 agttcatata tcacgatcaa atatgatgtt aacggaaatt tactctggtc aagagttcag
      781 ataagccagc aaattccata ttttcctgtt ctaaaactag ataaaaacag aaatgtttac
      841 atgtcctttg tgtctttcag accgggtaat ttttcgaatt atgctgtggt aaaatacaat
      901 aatgacgggg ttcagcagtg gatggcagaa tattataata caggatttag ccatatatat
      961 gatctaactc ttgatactca ggcaaatatt tatgtgaccg gtgcaagtgg gggtggacat
     1021 ggctattcca ttgcaacagt caaatttgtt caaaccccga cacaaataaa tcaatctaca
     1081 aataacatta taccggattc gtattggctc ggacagaatt tcccgaatcc gtttaatccg
     1141 aagacagtca tacagtatca gataccggtt aaaggattgg ttttgctaaa agtttttgat
     1201 gtgcttggga atgaagttgc ccaactgctg aacggtaatc aggaagcggg aagttacgaa
     1261 gcggaatttg acgggagcgg ttttgcgagt gggatttatt tttattcttt gtatttgaat
     1321 gagagtttgt ttgatacgaa gaggatggtt ttgctgaaat aattataaat tgtaaattat
     1381 aaattttaaa ttctaaaact tcggaagtct taattgattc cgaagttttt attttggttc
     1441 ttatttaagt gattctttaa tcatttaatc cattaatcaa gcttcagaca cattgaaaaa
     1501 ttccatagct tgtttcttaa aaacattcct cttacattaa atatatctta aaaaggaaat
     1561 actctatagg aagtaatgaa ttcattgaaa gcaataaaaa atgttaagag tcatcaggta
     1621 aatatgaaca cagcgattta agtttaatca ctttcctttt cggcaaaaaa cttcccagat
     1681 aagttttaaa atatttttta atttatatat ttttctttta tgaaaaaatc atcctcaata
     1741 tttataatta tctttataat gtgcagctcc ggtatttttg cacaggtaaa tcaggaatgg
     1801 gtcgcgactt acagcggaaa tggtttgggt aacaatgtgc ctaaaaaaaa cttagtcgat
     1861 aaagagaata atatatatgt agtgggaagg agctatggta ccggtgatga ttttataact
     1921 ttaaaatata atccatccgg gaatctgcta tgggaaaaaa gatataacgg gaccggtaac
     1981 ggagatgacg gaatatcagc agcaatcctt gatgattcta ataatattta tgtaacagga
     2041 tacagtcagg aaggagcagc tctcggcgga tataactgga taactataaa atattattca
     2101 aacggtgata cggcatggaa aagaagtttt aactggattg ctaacagtac tgatgaacct
     2161 ctcgcaataa ccatagacag atttaataat atatatgtag gcggatacgg aagatcgggt
     2221 cagcctatag atgatgactt tataactatt aaatataatt caaacggcga tctgctctgg
     2281 ctggattctt atgattcaaa tgtttttgga ccggacagag taatgagcat tgcaatagat
     2341 gattcatcca atgtctatcc aaccggttat tctgcttttt caggaggtaa tgaatagata
     2401 actataaaat ataatactaa tggtgaaaga aaatggataa gaaaaaattc aacatttaat
     2461 ggagattatt taagacctgc aagtgtaaca atagataaat atggaaatat tatagcaaac
     2521 ggatattatt atttattcgg aaactatgca tttattacaa taaaatataa ctctgaaggc
     2581 gatacattgt ggaagcgtat atacaaagga gacggtaatt taaatttttg ctttgcgctt
     2641 acgacagatg attccggaaa tgtatatgca gcgggaagga gtacaaatac tggaactgga
     2701 gaagactatg ttactataaa gtattattca gacggagaca ctgcatggat aagaaattat
     2761 gatggaggtt atgataactt tgatgaagcc agatctgtag ttgttgacaa ttatcagaat
     2821 gtatatgtaa ccggtttaac aagttcctct actggtagac caagctatac aacaattaaa
     2881 tataataagt ttggtgatat ttcctggata aaaaatttta gcggcagtta tttaactgat
     2941 gtagctacta gtttaaaaat agatataaat tataacgtaa tagttactgg atattcacag
     3001 acaagtataa attttgcttc aatagctaca ataaaatatt ctcaaataac gggtttcgtg
     3061 gataaaacta atttatttaa tttagatttt aaattatatc aaaattatcc aaatccattt
     3121 aatcccagta ccacaattaa atttacttta atatctgaaa gtaatgtaat actgaaaata
     3181 tataatgcac taggaaaaga aattgaaaca gtaattaata taaaaaaacc taaaggtact
     3241 catgtagtta tttttaatgg aaaaaatttt acaacaggta tatattttta cagcttattt
     3301 attaatggaa atttaataga tacaaaaaaa atgattttaa ttaagtaaga ttatgaaaaa
     3361 aataaaattt aagaacacct ttttaattgt tgtattttta tttaactatt cattcctgtc
     3421 agatatatca gctcaggtaa atcaggagtg ggtggcgact tacagcggaa ttggtttagg
     3481 aagcaatgct cctaaaaaaa acttagtcga taatgagaat aacatatatg tagtgggaag
     3541 gagctatggt acgggagatg attttataac tttaaaatat aatccttccg gaaatctgct
     3601 atgggaaaaa agatataacg ggatcggaaa cggtgatgac ggaatatcag cagcagtcct
     3661 tgatgattct aataatattt atgtgacagg atacagtcag gaaggagcgg ctctcggcgg
     3721 atataactgg ataactataa aatattattc aaacggtgat acggcatgga aaagaagttt
     3781 taactggacg gcgaatagta ctgatgaacc atttgcaatg actatagata aatccagtaa
     3841 tatttatatt tcaggatacg gaagaacagc acagcctttt gatgatgact ttataacctt
     3901 aaaatattca tcaaatggag actcggaatg gacaaaagta tacgcttcaa aatctctaaa
     3961 tcctgacaga tcaaacagta tcacagttga cgactccagt aatgtatatg cttcaggata
     4021 tggaggtact cctgataata atgagatcat tactataaaa tataattcaa gcggagtaga
     4081 aaaatggata aaaagatttc caacgaatta cgcagattta ttaagacata ctatatctgc
     4141 taatgataag aataataata taatagtaaa cggctactat tattttggtg atcactacgc
     4201 tttcaataca ttaaaatata attcacaggg tgatctatta tggaatcgaa tttataaagg
     4261 agacggtaat ttaaatttct gtcacgctct tactacagat gattcaggaa atgtgtatgc
     4321 tgcgggaagg agtacaaata ccggaactgg agccgatttt gttacaataa aatattattc
     4381 tattggggat actgcatgga ttagaaaata tgatggaggt tttaatcaat ttgatgaagc
     4441 ttactcaata gcagtagatg attatcaaaa tgtatatgta accggaatgt cagattcaac
     4501 ggatggtctt tcagattatt taactatgaa atatgattct gacgggaata ttaactggat
     4561 aaaaaagtat aatggatatt ttttagttga cagatcattc tgtttaagta ttgacaataa
     4621 ttacaatata attataagcg ggtttgctca aaaaaatata aatgatgcat ggataacttc
     4681 tataaaatat tctcagttta ctggcgtatc cggaaacgat atagattata attttaattc
     4741 ggttcttttt caaaattacc caaacccgtt taatccggct acaaatatta aatatcaatt
     4801 atactctacc ggaattgtat cgattaaaat ctatgacata attggaaaag aaataatatc
     4861 attaataaat aaacttcaga attcaggaac acatgaaata aaatttgata ttaataaccc
     4921 ggaaaataga ggtaatttac caagcggaat atatttttac agtcttttaa ttgacggaaa
     4981 aattattgac acaaaaagaa tgattttaat taaataagaa tatgaaaaaa atcaaaacga
     5041 tatgctttaa agagacctgc cgtgttaatt cacggcagga aaaattataa taaaa
//
LOCUS       DAORVQ010000227         4936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12094_length_4936_cov_5.851875, whole genome shotgun sequence.
ACCESSION   DAORVQ010000227 DAORVQ010000000
VERSION     DAORVQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12094_length_4936_cov_5.851875"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..2443
                     /locus_tag="PLX80_13130"
     CDS             224..2443
                     /locus_tag="PLX80_13130"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY21778.1"
                     /translation="MICIYRCAFLCVLIIFCCPFESKAQLKLYENFESGEFPPSGWTV
                     VNTAGDDGEWKLNNRNTYLGNGCAVSNFSIYSSKNYLISKRLTPASGDSLIFYFKQTF
                     WNNYNDTFNVLLSNTDSLIQSMGTVLRIFNENINYPNYLEYGRYSISLNAFAGQTVWI
                     AFQHKNKNGDNIRLDDISVGTPGQVEVSVVNNIFPIGKFGSCSNEFLIPSASIRNNSS
                     ENITTNFNVTYKITGPVNFTSTKPIIINGGELKVVNFDTADISVPGIYNVKIYSSLPN
                     DPNKNNDTIYSYFEIVQADYGGGIFYNGGYYFSNSTECAANAQSKPQFSWKDTINSVN
                     LILNKQRNSSIPFTGNTNDGYFSVGNIMPTGYSFRYYGQNFDSVFISTNGIIGFKKND
                     ALLSSDPTKINSLFFVQLPAICAFWADMDFGSTLSPNNRLSYKVAGKYLVITYDKAHL
                     RSGEATEYLSFQISLELVNSPAANSRFILQYQKESTGQNFLENYSDNTLRPHFVGFKN
                     TAGNFYLKYRFKDSTGLIDHGTLFNSSVAAEFGASQLNLNNKSSDLNLTAKFEGFTPR
                     RDTVTIQIRDTYYPYKILESKKIYMNSNGTLTGKFSIPAENYQYYIALKHRNSLETWS
                     KANGEFFTGFNLNYNFSTSASNAYGSNMRFVNGSYYIYSGDINEDGLIDLSDYSKVSN
                     DAENFLTGYNISDINGDGLVDLADILFTDNNAFNFIKHNTPLVIDQNLIPGSTNSGK"
     gene            2605..3558
                     /locus_tag="PLX80_13135"
     CDS             2605..3558
                     /locus_tag="PLX80_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQY21779.1"
                     /translation="MFNIEEPELKETRQGFADALLELGAEIPNLVVLGGDVTGSVKTN
                     LFRDAYPDRFISVGIAEQDMIGTAAGLALAGKIPVASTYGEFATGRPFDQIRQSVAYS
                     EMNVKICASHCGITVGPDGATHQSFEDVGIMRILPNMNVLTPCDYNETFNAVKTAIKT
                     VGPFYVRFYREKSPNFTDIKKEFKIGKADTLIDGSDVTLIASGLLVWEAILASEKLKK
                     EGISARVVNINTIKPIDKDTIIKCAKETGLIITCEDHQWQCGLFGAVAEVTALHHPVK
                     MDYIAIDNTFGESGIIFSELMSKYKIDSAAIYNKVLSHLKK"
     gene            3578..4810
                     /locus_tag="PLX80_13140"
     CDS             3578..4810
                     /locus_tag="PLX80_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQY21780.1"
                     /translation="MNNNLISDTVNIQNNKNYRSVNTTGKILLAILTVLILTSGVLIG
                     KYFLSPGGNENTIVNSTQNSLQENNNNISETRETAITRAVKKVSPTIVGINVEEVREV
                     QDPFSMFDNDPFFKQFFGNRQPQKRVVRGLGSGYIISSDGYILTNDHVAGNATKISVT
                     MTNGETIDARLIGSDKNSDVALLKIDKNNLPFATLGNSNDVIIGEWVIALGNPFGLFD
                     INDQPTVTVGVVSATDMKISTGEGNRVYKEMIQTDASINSGNSGGPLLNADGEVIGMN
                     TIIYTGGQYSGGSIGVGFAISINRVKKIMEEIKEFGVIDRNFNVGFRIQQIDERIAKY
                     LNLSSTEGVVVTDVLKGGLSDKAGLQSEDVIIEANGEKVKNDQDILFIVNDLKKGENL
                     KLKIIRKGSEKDIDFKLE"
BASE COUNT         1786 a          716 c          889 g         1545 t
ORIGIN      
        1 aaggacttta taataaaaat aaaactaaaa ttatataata atttatattt ataaataaat
       61 aaaatatcga atttttgatc ttgctgaatg acgtataaaa ttcagagaat caaatacatt
      121 atcatcctga taagactgaa ccggagcaaa gtaatcccct gcttttaacc ggtacaaaga
      181 tttaacccca aagaatattt ttacaaacta aaaaggagat tttatgatct gtatatacag
      241 atgcgctttt ttatgcgttt taattatttt ttgttgccct ttcgaaagta aagcacagtt
      301 aaaactttac gagaattttg aaagcggaga attcccacct tcgggatgga cagttgtaaa
      361 cactgccgga gacgacggtg aatggaaatt aaacaaccgt aatacgtatt taggaaatgg
      421 ttgtgctgtt tcaaattttt ctatctacag ttctaaaaac tatctcattt ctaaaagatt
      481 gacacctgca tcaggtgatt cacttatttt ttattttaag caaaccttct ggaataatta
      541 taatgatacg ttcaatgtac ttctttccaa tactgattct ctcattcaaa gtatgggtac
      601 agtacttcgc atttttaatg aaaacattaa ttatccaaat tatttagagt atggaagata
      661 ttctataagt ctgaatgctt ttgctggtca aactgtatgg atcgcatttc aacataaaaa
      721 taagaacggt gataatatca gattagatga tattagtgta ggtactcccg gacaagttga
      781 agttagtgtg gtgaataata ttttcccaat aggaaagttt ggttcctgtt caaatgagtt
      841 tttaattcca tctgccagta taagaaataa cagctcagag aatatcacaa ctaattttaa
      901 tgtcacatat aaaattacag ggcctgtaaa tttcacaagc acaaaaccta taattataaa
      961 tgggggagag ttaaaagtag taaattttga tacagctgat atttccgttc ccggaatata
     1021 taatgtgaaa atttattcat ctttgccaaa tgatccgaat aaaaataatg acacaatcta
     1081 ttcttatttt gaaattgtac aggcggatta cggtggcggt atattttata acggaggata
     1141 ttatttctca aattctacag aatgtgcagc aaatgctcaa agtaaacctc agttcagttg
     1201 gaaagatact atcaatagtg taaatctcat tttaaataaa cagagaaaca gcagcatacc
     1261 atttacagga aatactaatg acggatattt tagtgttgga aatattatgc caacaggata
     1321 cagttttcga tattacggtc aaaattttga ttcggtattt atatctacaa acggtataat
     1381 tggatttaaa aagaatgatg ctcttttatc ttcagacccg acgaaaatca attcactgtt
     1441 ttttgttcag ctgccggcta tttgtgcatt ctgggctgat atggattttg gcagcacctt
     1501 gtctccaaat aacagattaa gttataaagt agcaggcaaa tatcttgtga tcacttatga
     1561 taaagctcat ctaagatcag gtgaagctac agaatattta agttttcaga tctcacttga
     1621 gcttgtgaat agtcctgcag caaattcaag atttatatta cagtatcaaa aagaatctac
     1681 aggacagaat tttcttgaaa actattcaga caatactctt cgtccacact ttgttggatt
     1741 taaaaacaca gcaggaaatt tttatttgaa atacagattc aaagattcta caggattaat
     1801 agatcacgga acattgttta attcatctgt tgccgctgag tttggagcta gtcagttgaa
     1861 tttaaataac aaatcttctg acctgaacct tacagcaaaa tttgaaggat tcacacctcg
     1921 gagagatact gtaactattc agatccggga tacatactat ccatacaaaa tattggaaag
     1981 taaaaaaatc tatatgaaca gcaacggtac attaaccgga aaattttcca tacctgcaga
     2041 aaactatcaa tattatattg cactaaaaca cagaaattca cttgagacat ggagtaaagc
     2101 aaacggtgaa ttctttacag gatttaatct gaattataat ttcagcacca gcgcttcaaa
     2161 tgcttatgga agtaatatga gatttgtcaa cggtagttat tatatttact caggagatat
     2221 taatgaggac ggtttaatag acctgtcaga ttattcaaaa gtatctaatg atgcagaaaa
     2281 tttcttaaca ggatataaca tatcggatat taatggtgat ggacttgtag atctggctga
     2341 tatacttttt acagacaata atgcttttaa ttttataaaa cataacaccc ctttggttat
     2401 tgatcaaaat ttaattcccg ggagtacaaa ttcgggaaag taaaccaaaa attttagatc
     2461 agaataaaag caaagccggg ctataatgtc cggcttttgt tttaataagt aataagctgt
     2521 caatagtagt gatttatgat ttgaaatgaa ctcttaaata aagtattttt gaaattataa
     2581 aattttttaa aagttttaaa tatgatgttt aacatagaag aacctgagtt aaaagaaaca
     2641 agacagggat ttgcagatgc tctgttagaa ttaggtgcag aaataccgaa tctcgtagta
     2701 ctgggcggag atgtaaccgg ttctgtaaaa actaatcttt ttagagatgc ataccccgac
     2761 agatttattt ctgtaggaat agccgagcag gatatgattg gtacagcagc aggactagca
     2821 cttgccggta aaattcccgt tgcttctact tatggagaat ttgcgaccgg aagacctttc
     2881 gatcagatca gacaatctgt tgcatacagt gaaatgaatg ttaagatctg cgcatcacac
     2941 tgcggaatta ctgtaggacc tgacggtgct actcatcagt catttgaaga tgttggtata
     3001 atgagaatac ttcccaatat gaatgtgctt accccatgcg attataatga aacttttaat
     3061 gcagtaaaaa cagctattaa aactgtagga cctttttatg taagatttta cagagagaaa
     3121 tctccgaatt ttactgatat caaaaaagaa tttaagatag gaaaagctga tacattgatc
     3181 gatggcagtg atgttacttt gattgccagc ggtcttttgg tttgggaagc aattttagct
     3241 tctgaaaaac tgaaaaaaga aggaatcagt gcaagggttg taaatataaa tacaattaaa
     3301 ccaattgata aagatactat tattaagtgt gcaaaggaaa ccggtcttat cataacatgt
     3361 gaggatcatc aatggcagtg cggtttgttt ggcgcagttg ctgaagttac agctttacac
     3421 catcccgtaa aaatggatta tattgctatt gataatacat tcggagaaag cggaataatc
     3481 ttttcagaac ttatgagtaa atataaaatc gacagtgctg ctatttataa taaagtacta
     3541 agtcacttaa aaaaataata tagttttact aaataaaatg aataataatt taatttcgga
     3601 tactgtaaat attcaaaaca ataaaaatta tagatctgtg aatacgaccg gcaaaatact
     3661 tcttgcaatt ttaacagttc tgattcttac atccggagtc ctgatcggta aatatttttt
     3721 atccccgggt ggtaatgaaa atacaattgt taattcgaca cagaattctc tgcaggaaaa
     3781 taataacaat atttcagaga caagagaaac tgcaattaca agagcagtaa aaaaagtttc
     3841 tccaacaata gtaggaataa atgttgaaga agtaagagaa gtgcaggatc ctttttcaat
     3901 gtttgataat gatcctttct ttaaacagtt ctttggaaac agacaacctc agaaaagagt
     3961 cgtcagagga ttaggttcgg ggtatataat ttcttctgat ggttacattc ttaccaatga
     4021 tcacgttgca ggtaacgcca caaaaatatc tgttacaatg acaaacggag aaacaataga
     4081 tgcaagatta atcggttctg ataaaaacag cgatgtagct ctgttgaaaa tcgataaaaa
     4141 taatcttcca tttgcaacat taggaaattc aaatgatgta ataataggtg aatgggtaat
     4201 tgcacttggt aatccatttg ggttatttga tataaatgat cagcctactg tgacagtcgg
     4261 agtggtcagt gcaacagata tgaagatttc tacaggagaa ggcaacagag tatataaaga
     4321 aatgatacag acagatgcat ccattaattc aggaaattca ggcggaccac ttttaaatgc
     4381 tgatggtgaa gtgattggaa tgaatacaat tatttacaca ggcggtcaat acagcggcgg
     4441 tagtatcggt gtaggttttg caatttccat taacagagta aaaaagataa tggaagagat
     4501 aaaagaattt ggtgtaatcg acagaaattt caatgttgga ttcagaattc aacagataga
     4561 tgaaaggatc gctaaatatc ttaatcttag ttccacagaa ggagttgtag ttactgatgt
     4621 tttaaaaggt ggactatctg ataaagcggg tcttcaatct gaagatgtaa ttattgaagc
     4681 aaacggagaa aaagttaaga atgatcagga tattctgttt atagtaaatg atctgaaaaa
     4741 aggtgaaaat cttaaattga aaattatcag aaaaggatct gaaaaggata tagattttaa
     4801 actggaatag taagattttt atagaaacaa aaaaggcttt gatcataatc aaagcctttt
     4861 tttatttgaa aaaaaattat ttattaatga aatttcaaaa caggtttatc tttttctttt
     4921 gattcatcct gtattt
//
LOCUS       DAORVQ010000228         4917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12185_length_4917_cov_5.235911, whole genome shotgun sequence.
ACCESSION   DAORVQ010000228 DAORVQ010000000
VERSION     DAORVQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12185_length_4917_cov_5.235911"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..2454)
                     /locus_tag="PLX80_13145"
     CDS             complement(331..2454)
                     /locus_tag="PLX80_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21781.1"
                     /translation="MNKIIFYTIVLMFLICSGNSYSQLLSIAEQDKKVNVTEEIMTVP
                     ANKEFSHTAEGLKIVNEMIRLKSEVKTGNGEKLLELQKKLERIEYTSVTKTFDAGELR
                     FSDNHNQKTNQFHTDAITISAIYSTYNSYIKAIATQVEQTAPGTGTIWVAFAVGTSDS
                     GAGAAPDTLLLYKSVNDGLIYSLYSRLTLSTGNKINSDEMDMEIIESPASKFIYLTAG
                     FSTNGYTGVYRNALVVFDIVNNVSSSVVLNFPGTVSSSKYYRARITSDNARYALNAYV
                     TISVIQDSTDGFNHYYMTKFCRIINPYTTTPAITYLPAALYLPLIYAPFSSTVHADIA
                     YYNTGGNAAGDSLIFVLSGFPGALEKIFIYKGYSNTAVYPALSKILSGTLNDKQFVRV
                     ASTGGNDQKKMMMIFMESEGSVNSSLYTYTTSDANNWSKSLVQGGAFGVFQLKNSDIT
                     GQRNVSGKFYAAFKIDSPLIDIIGSMGYNDLTRTSFVLNHNNIGGKTDASPKPAFRYN
                     NTDSCLTIWSAASSVNSSCGCSVINAAVRFYIQGLYDEITNLTKYDLAKIYLRNSVSP
                     YSKVDSALGLSGTIFTFHNANPGTYYVSVTHRNSVETWYYQPVALVYSNNVSVDMMTI
                     QSRAFGSNQIQIDNSPVRFGIYSGDVNQDGTVDLTDGSLIDNDAINFASGYLPTDLNG
                     DGIIDVADAVFADNNSFNFVSKITP"
     gene            complement(3002..3487)
                     /locus_tag="PLX80_13150"
     CDS             complement(3002..3487)
                     /locus_tag="PLX80_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21782.1"
                     /translation="MGNPKTYSSDNQDPQGTAGIQGNSSIFNGVLGYTTAEGHAGVAG
                     ACDEGLGNGVYGRSKNRVGVYAVSENYEAVHAVTNSSKTAAIAAYNLNVNSDAPSLYA
                     KKRGKGCSAVFDGKVNIVEGDLKILKGTIYIKDVDIVDLLYKLSAEIAQLKSRVMILE
                     I"
     gene            complement(3555..3965)
                     /locus_tag="PLX80_13155"
     CDS             complement(3555..3965)
                     /locus_tag="PLX80_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21783.1"
                     /translation="MLKPQLVILVVMLTILMSFSNSNSQVIVSEDSSGSVIDQSGNDL
                     EPGKKICKLYHSKKTEIKNVMVIEFDLDEEADVKLEVCNSKGQIIEILIDEVMDPGNY
                     KIHFKTTDEIIPEEHTYKLEVKGISGLRNVLNEK"
     gene            4215..>4917
                     /locus_tag="PLX80_13160"
     CDS             4215..>4917
                     /locus_tag="PLX80_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21784.1"
                     /translation="MLKSSLLEILRTFTKQELIKFEDFVRSPYFNKKENVTKLFLEIK
                     KYTPEFSNENLEKERVWGNIFPGKEYNYGIMKNLIHELTKLSESFITIEYSKLDKLEN
                     SIDLLVTLMERQATKVFSSKIDMIEKIYNNPDLKSERYGIENYYEFLDKLYYLKSQYN
                     WHHNMKSTAAYECTSRCTDYLLYSAIIYFYKYYNNYLAHNNKEVWNKSNAADIFLAGL
                     NNNLISQLLENVKVKS"
BASE COUNT         1604 a          865 c          774 g         1674 t
ORIGIN      
        1 gttaatagtt aatagttaat agttaatagt taatagttaa tagttaatag ttaacaatta
       61 atagttagca gtgcatcaat ccaataacca ggatcaaaat ttaaaaatat aaaatcagcc
      121 ctgcataccg gcgatgcaat taagtgaata tgttcgcgga caattcacgg gctggtttta
      181 atcaatttca aattacaaat gtcaaatatt taattagaga ttacatcttt aaatttatcc
      241 tcaagtaaga tttaaaaccc attatttgaa aacccgtaat tcgcaactgt ttttaataac
      301 tgttaactct taactactaa ctattaactg ttaaggagtt attttactca caaagttaaa
      361 agagttatta tccgcaaaca ctgcatcggc aacgtcaata attccgtctc cgtttaagtc
      421 agtcggcaga tatcctgaag caaaatttat agcgtcgtta tctatcaggc taccgtctgt
      481 taagtcaacc gtcccgtcct gattcacgtc tccgctgtaa attccaaatc ttacaggcga
      541 gttatcaatc tgtatttgat tgcttccaaa tgctctcgac tgaattgtca tcatatcgac
      601 actaacatta tttgaataaa ctaaagctac aggctgataa taccaggttt caacagaatt
      661 tctatgtgta acagagacat aataagttcc gggattcgca ttatgaaatg tgaatattgt
      721 tccgcttaac cctaatgcag agtcaacttt gctgtaagga gagacgctgt tccttaaata
      781 aatttttgca agatcatact tggtaagatt agttatttca tcatataaac cctgtatata
      841 aaatctaacc gcggcattga tcacactgca tccgcatgat gaattaactg atgaagctgc
      901 gctccatatt gtcagacagc tgtcggtatt gttataacga aatgcaggct taggtgaagc
      961 atcagttttt cctcctatgt tgttgtgatt taatacaaag gaagttcggg tgagatcatt
     1021 atatcccatt gagcctatga tatctattag tggcgaatca attttaaatg cagcataaaa
     1081 tttaccggag acatttctct gtccagttat atcagaattt ttcagctgaa aaacaccgaa
     1141 tgcgccgccc tgtactaatg actttgacca gttatttgca tctgaagttg tataggtata
     1201 caacgacgaa ttaacagaac cttctgattc cataaagatc atcatcattt ttttctgatc
     1261 atttccgcct gttgatgcga ctcttacaaa ttgtttatcg ttaagtgttc ctgatagtat
     1321 tttagaaagg gcaggataaa cggcagtatt actgtagcct ttgtaaataa atatcttttc
     1381 caatgctccg ggaaatccgc tgagaacaaa aatcaaactg tctcctgctg catttccacc
     1441 tgtattgtag tatgctatat ctgcatgtac agtgctgctg aacggagcat agatcaatgg
     1501 taaataaaga gcagcaggca gataagtaat tgccggtgta gtagtatatg gattaattat
     1561 ccggcagaat ttagtcatgt aataatgatt aaagccatct gtagaatcct gtattacgga
     1621 aattgttaca tatgcattta atgcatatcg tgcattatct gaagttattc gcgccctgta
     1681 atatttggac gaagatacag tcccggggaa atttaataca actgaagatg aaacattatt
     1741 aacaatatca aatactacaa gcgcatttct atatactccg gtatagccgt ttgtactgaa
     1801 tccggcagta agataaataa actttgaagc cggagattct attatttcca tatccatttc
     1861 atcgctgttg atcttatttc ccgtacttaa tgtcaatctt gaatataaac tataaattag
     1921 accatcgtta accgacttat aaagaagcaa agtatccgga gctgctccgg cacctgaatc
     1981 agaagttcct acagcaaacg caacccatat tgtgccggtc ccgggagctg tttgttccac
     2041 ttgtgtagct atagctttga tatatgaatt ataagttgaa taaattgctg atattgtaat
     2101 tgcatcagtg tgaaactgat ttgttttttg gttatgattg tctgaaaatc ttaattcacc
     2161 tgcatcaaaa gtttttgtta cggaagtata ttctattctc tcaagttttt tctgaagttc
     2221 taaaagcttt tcaccattcc cggtttttac ctcactcttt aaccgtatca tttcattaac
     2281 tattttaagt ccttcagccg tatgactgaa ttccttattt gctggaacag tcattatctc
     2341 ttctgttaca tttacctttt tgtcctgttc cgcaatactt aagagctggg aataggaatt
     2401 tcccgaacaa attaaaaaca ttaatacgat tgtataaaaa ataattttat tcatttatat
     2461 aaggttgatt ttttaaaaat tataaaatag ttagtagtta atagttaata gttagtagtt
     2521 aatatttatt tgataataat ttcctgataa atttaaaatc tacatccggc ttgcattatc
     2581 aggacattca taaaataaaa aaagccctgc ataccggcga tgctattaag tgatatgttt
     2641 cgcgtacaat tcacgggctg attttttaat gaacagttac aagttacaat tttggatttt
     2701 tgtatctgaa atcttcaatc aaaattccaa aatcaaaaaa atacaaattc aaaattccta
     2761 aattcccaaa gcccgtactg atcgggggat cctgtcattt tcaaatatga cttacgggct
     2821 gttgatatgg gaaaaaacta tgatacttca aatcttaaat ctgaaattta aaatcagccc
     2881 agcataccgg cgatgctatt aagtgaatat gttcgcggac aattcacggg ctggttttta
     2941 aaagaacaat tactaattac gaattacgaa ttacgaaaaa ttttgtatag atctaacggt
     3001 gtcatatttc caggatcatt actctacttt tcaactgagc aatttcagca gacaatttgt
     3061 ataataaatc aacaatgtcg acatctttta tgtagattgt tcctttcaaa attttcagat
     3121 caccttccac aatgtttact ttgccgtcaa atactgctga acagcctttg cctctctttt
     3181 ttgcatataa agacggagca tcactgttta catttaaatt gtaagctgca atagccgcag
     3241 ttttggatga gttagtcact gcatgtacag cttcataatt ctcacttaca gcatatacac
     3301 ctacgcggtt tttacttctg ccgtaaactc cattacctaa accttcatca catgcccctg
     3361 ctactcctgc atgcccttcg gctgttgtat aacccagtac tccgttgaat attgagctgt
     3421 taccctggat ccctgctgtt ccctgtggat cctgattatc tgaactgtaa gtttttggat
     3481 ttcccatttt ttttattttt ttaatttatg attatagagc agtttaaaat tttgaatttt
     3541 ttagaaaaaa tgctttactt ttcattgaga acatttctta atcctgaaat accttttact
     3601 tccagtttgt aagtgtgctc ttcggggatg atctcatctg ttgttttaaa atgaattttg
     3661 taattccccg ggtccattac ttcgtcaata agaatctcaa ttatctgacc tttcgaattg
     3721 caaacctcaa gctttacatc cgcctcttca tccagatcga attcaataac cattacattt
     3781 ttaatctctg tcttcttact gtgataaagt ttacagatct tttttccggg ttccagatca
     3841 ttccctgatt gatcaattac agaacctgat gaatcttctg atacaattac ctgcgaattt
     3901 gaattagaaa aactcatcag gattgtcagc atgacaacta aaattactaa ttggggtttt
     3961 aacatttttt atctcctttg ttttagtgtt tcaatttaat tgatacaaaa atatatcaga
     4021 aatttttact gtcaaaagaa actttttcac gagtttcaca gataagtact tacatttttt
     4081 aaataaaagt cttattttgt gctgtaattg aaataaaata ttaaagacct tcttagctgt
     4141 gagtttaatt atgagtatat acactcatat aaaattctct gaaaaggaag taagtttata
     4201 tcaatttttt aaatatgtta aaaagctcac ttcttgagat cctccgcaca ttcaccaaac
     4261 aggagctgat aaagtttgaa gactttgtca gatcgcctta ttttaataaa aaggaaaatg
     4321 taacaaagtt atttcttgag ataaagaaat acacgccgga attttcaaat gagaatctgg
     4381 agaaagaaag agtatggggg aatatttttc cgggaaaaga atataattac gggattatga
     4441 agaatctaat tcatgaactt accaaattaa gtgaatcatt tattacaatt gaatattcaa
     4501 aactcgataa actggaaaac agtatagatc ttttggtaac attaatggaa agacaggcca
     4561 caaaagtatt ttcatcaaaa attgacatga ttgaaaaaat ttataataat cctgatttaa
     4621 aaagcgaaag atatggtatt gaaaattatt atgaattctt agacaaacta tattatctga
     4681 aatcacaata taactggcat cataacatga aatcgacggc agcatatgaa tgtacaagcc
     4741 ggtgcacaga ttatctatta tactcagcta tcatatattt ttataaatat tacaataatt
     4801 atctcgcgca taataataaa gaagtttgga ataaatcaaa tgcagccgac atatttctcg
     4861 caggtctgaa taataattta atttctcagt tattagagaa tgtgaaagtt aaatctg
//
LOCUS       DAORVQ010000229         4916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12190_length_4916_cov_5.105328, whole genome shotgun sequence.
ACCESSION   DAORVQ010000229 DAORVQ010000000
VERSION     DAORVQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12190_length_4916_cov_5.105328"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..481
                     /locus_tag="PLX80_13165"
     CDS             68..481
                     /locus_tag="PLX80_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21785.1"
                     /translation="MHQLKEENIGKIEGLFSEKFIRSENSYTINIPHKNINSNICIDI
                     YPEEDDKSLISVYTNNTHLQLQSCSNMIISDMLEEIIFISETEEHISGLIVSKSGDCS
                     MYSNVSKKVLKTDFSELNSEKLLAAIALSVSETMI"
     gene            512..970
                     /gene="ybeY"
                     /locus_tag="PLX80_13170"
     CDS             512..970
                     /gene="ybeY"
                     /locus_tag="PLX80_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQY21786.1"
                     /translation="MEINIIYSDGCSNNLQKKEIRKSIKEISSLIIKEENINPEILNF
                     IFCDDKMIREYNKKYLGHDYETDILTFFEEDDNGVKEGELLISIDTVRSNSEYFKTEF
                     SKELQRVVIHGMLHLSGYKDSKISERKAIRKKEDYYLKTLNNLNNAGKNI"
     gene            951..1406
                     /locus_tag="PLX80_13175"
     CDS             951..1406
                     /locus_tag="PLX80_13175"
                     /inference="COORDINATES: protein motif:HMM:NF016264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HQY21787.1"
                     /translation="MQEKIFELIVNILNEMQETNEFNEIDIKKYTSEGYTKAEINTAF
                     AWIFSKIESSELIFNADRKNSRSHRVYHKFEKNLFAKEALGYMIQLKELGIITETDEE
                     SIIDKLISAGYKDIGIDEIKMIASTMIFLSSDPDNRLNRLILQNNDTIN"
     gene            1446..3794
                     /gene="topA"
                     /locus_tag="PLX80_13180"
     CDS             1446..3794
                     /gene="topA"
                     /locus_tag="PLX80_13180"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQY21788.1"
                     /translation="MKKSLVIVESPSKAKTINKYLGKDFIVEATVGHIKDLPKSKMNV
                     DLENDDFTATFGVIPGKENIINNLKRLASSSDKVYIATDPDREGEAIASDIADEIVNK
                     KNKIQRVLFNEITKSGIEKAMSSGRKIDENLVSAQVARRVMDRILGYKVSPFLWKTFY
                     FGLSAGRVQSVALRVICEREEEIEKFKPKEYWSIEGLFSKPSGNSKSFRAKLFKINDD
                     TLKFDGENPCIRDIDHAYIIMQELRDNKYKITDIQVKEVKRNAPAPFTTSVLQQTASS
                     RLGFSPKKTMMLAQKLYEGIEGNKEDGLVGLITYMRTDSTRVSGEAVDSARVFIDENY
                     GKDYLPEKPKEFKTKKKNTQDAHEAIRPTDVNRKPEDMKKLGKDLFALYDLIWKRFVA
                     SQMSQAVLDQKTVIITALSPRGCKNKYTFKATGSVMKFSGFLKVYEDNSEDLNDKPDN
                     AIAEDDITLIPSDLSIDDFLKVINLNQEQHFTNPPPRYTESSLIKQLDKLGIGRPSTF
                     AAIVSTVINRMYVELKEKKLYATNLGKAVNKILSEHFKDVINANFTAKMETELDTIAS
                     GESSYKKVMNEFYDPFNSDLIVADSIAAEIKKSLIEKTVIDCPECGKETGAKMIKKWS
                     RNGQFLSCETFPKCKGALPLELPTEKDLEMAKGIICDICQAQMTIKVGKYGKFYGCTN
                     YPKCRGIKPLTLGIACPKCDKGEILQRKAKGGRFFYGCTKYPDCDFIANTMPVIQDCT
                     NCKSKYLLKKSSKKDGDYLECPECKSRYEDETVNEKELSEKD"
     gene            3823..4755
                     /locus_tag="PLX80_13185"
     CDS             3823..4755
                     /locus_tag="PLX80_13185"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HQY21789.1"
                     /translation="MSKSSEDHIKSFLTYIKINRNYSDNTVISYENDLSQLIEYAEKS
                     GFITAKDKTDFNDLDLSFLKSYVAEISDPFKRKGKYSKKSISRKISVIKSFYKYLYRK
                     KYISKNYSSSLIFPKLNKKLPSILSESELNKLLGEKYLAELSILDKAIIELFYSTGIR
                     LSELINLKFHNIDLTNKTIKVKGKGSKERIVPFGREADISIKNYLKIRDICNVNSIDN
                     LFMDNAGKILYPMKINRMIKKNLSLVTQSSKKSPHVLRHSFATHLLDNGADIRAVKDL
                     LGHESLSTTQVYTHITPERLKKVYRQAHPRSKEL"
BASE COUNT         1939 a          710 c          867 g         1400 t
ORIGIN      
        1 agaaaacctt gattttatat aaacaggatt aaaattcttt aaataaaaaa aaaacgaata
       61 atttaaaatg catcaactga aagaagaaaa tatcggtaaa attgaagggt tattttcaga
      121 gaaattcatt agatcagaaa attcttatac aataaacatt ccgcataaaa acataaattc
      181 caacatttgt attgatatat atcctgaaga ggatgacaaa tcattgattt cggtatatac
      241 aaacaataca catttacaac ttcagtcctg ttcaaatatg atcataagtg atatgcttga
      301 agagattata tttatttcag agacagagga acatatctca gggttgatag tttcaaaatc
      361 cggcgactgt tcaatgtatt ctaatgtcag caagaaagtt ttgaaaaccg atttttccga
      421 gttaaattct gaaaagctgc tggctgccat agcattatct gtttcagaaa caatgatctg
      481 aatttaaaat tttttaaatt agatttcaaa tttggagata aatataattt attctgatgg
      541 ttgtagtaat aatttacaaa agaaagaaat aagaaaatca ataaaggaaa tttcatcttt
      601 aataataaag gaagaaaata taaatcccga aatattaaat tttatctttt gtgatgataa
      661 gatgatcaga gaatataaca agaaatatct tggacatgac tatgaaactg acatacttac
      721 attttttgaa gaagatgata atggagttaa agaaggagag ttactcattt caattgatac
      781 agtaagaagc aattcagagt atttcaaaac agaattttca aaagagcttc aaagggtggt
      841 aattcatgga atgctgcact taagcggtta taaagacagt aaaatttcag aaagaaaagc
      901 aatcagaaaa aaagaagatt attatttaaa aactttaaat aatttgaata atgcaggaaa
      961 aaatatttga actaatagta aatattctca atgaaatgca ggagacaaat gaattcaatg
     1021 agatagatat aaagaaatac acaagcgaag gttatacaaa agcagagatc aatacagcat
     1081 ttgcatggat attttcaaaa attgaaagca gtgaacttat ctttaatgca gacagaaaaa
     1141 actcaaggtc acatagagtt tatcataaat tcgaaaaaaa tttatttgca aaagaagcgc
     1201 ttggatatat gatacagcta aaagaactgg gtataataac tgaaacagat gaagagtcca
     1261 ttatagataa actgatttct gccggatata aggatattgg aattgatgaa atcaaaatga
     1321 tagcctcaac tatgatcttt ctttcatccg atcctgacaa caggttaaac agattaattc
     1381 ttcaaaacaa tgatacaata aactgatatt ataaacacat aaattttaac gattaaaaat
     1441 atttaatgaa aaaatcttta gtaatagttg agtcaccttc caaagcaaag acaattaaca
     1501 aataccttgg taaagacttt atagtagagg caacagtcgg acatatcaaa gatcttccta
     1561 aaagtaaaat gaatgtagat ctggagaatg atgattttac ggcaactttc ggtgtgatcc
     1621 ccggtaaaga aaatataatc aacaatttaa aaagattagc atcatcttct gataaagtat
     1681 atattgcgac tgaccctgac cgtgaaggag aagccattgc ttcggatatt gcagatgaga
     1741 tcgtgaataa gaaaaacaag atacagagag ttctttttaa tgaaataacg aaatccggaa
     1801 ttgaaaaagc tatgagcagc ggcaggaaga tagatgaaaa tctggtttct gcacaggttg
     1861 caagaagagt gatggacaga atacttggat ataaagtaag tcctttttta tggaaaactt
     1921 tttactttgg attatcagca ggtagagtac agtctgtggc attaagagtt atatgtgaga
     1981 gagaagaaga gatagagaaa tttaaaccaa aagaatactg gtcgatcgaa ggacttttct
     2041 caaaaccatc cggaaattca aaatcattca gagctaaatt atttaagatc aatgacgaca
     2101 cactaaaatt tgacggtgag aacccatgta tcagagacat tgatcatgct tatataataa
     2161 tgcaggagct tcgtgataat aaatataaga tcactgatat tcaggtaaaa gaagttaaga
     2221 gaaatgcacc tgcacctttt actacttcag ttttgcagca gacagcatct tccagacttg
     2281 gattttctcc aaagaaaacg atgatgcttg cgcagaaatt atatgaaggt attgaaggaa
     2341 ataaggaaga cggacttgta ggacttatta cttatatgag gacagattca acaagggtaa
     2401 gtggagaagc agtagactca gcaagagtat tcattgacga aaattatggt aaagattatt
     2461 tacctgaaaa gccaaaagaa tttaaaacaa aaaagaaaaa tactcaggat gcacatgaag
     2521 cgataagacc taccgatgtt aacagaaaac cagaggatat gaaaaagctc ggcaaagatc
     2581 tgtttgcatt gtatgatctt atctggaaaa gatttgtggc atctcaaatg tctcaggcag
     2641 tacttgatca aaagacagtt attattacag ctctttctcc gcgtggttgt aaaaataaat
     2701 atacattcaa agctaccggc agtgtaatga aattcagcgg attcctaaaa gtgtatgagg
     2761 ataattctga agacttaaat gataaacctg ataatgctat tgctgaagat gacataactc
     2821 tgatcccatc tgatctttca atagatgatt ttcttaaagt aattaatctg aatcaggaac
     2881 agcattttac aaacccacct ccgagatata ctgaaagtag tctgataaaa cagttagaca
     2941 aattgggtat cggcagacca agtacatttg cggcaatagt ctcaactgta attaacagaa
     3001 tgtatgtgga gctaaaggag aaaaaattat atgctacaaa tctaggtaag gcagttaaca
     3061 aaattttatc tgagcatttc aaagatgtaa taaatgcaaa ctttactgcg aagatggaaa
     3121 ctgaacttga tacaatagcc agtggagaga gttcttataa aaaagttatg aatgaatttt
     3181 acgatccgtt taattctgat ctgatagttg cggattcaat tgcagcagag atcaaaaaaa
     3241 gtctaataga aaaaacagtt atcgactgtc ccgaatgcgg aaaagaaacc ggtgcaaaaa
     3301 tgataaagaa gtggagccga aacggacagt tcctgtcctg tgaaacattc ccgaaatgta
     3361 aaggtgcact gccacttgaa ttgccgacag agaaagatct tgaaatggca aaaggtatca
     3421 tctgtgatat atgtcaggca caaatgacaa tcaaagtcgg taagtatgga aaattttatg
     3481 gatgcacaaa ctatccgaaa tgcagaggga ttaaaccact gactctggga attgcttgtc
     3541 ctaaatgtga taaaggagaa atacttcagc gtaaagcaaa aggcgggaga tttttctatg
     3601 ggtgtacaaa atatccggac tgtgatttta ttgcaaacac aatgcctgtg attcaggact
     3661 gtactaattg taaaagcaaa tacctcttaa agaaatcttc aaagaaagac ggagattatc
     3721 tggaatgtcc tgaatgtaaa agcagatacg aagatgagac tgtaaatgaa aaagaacttt
     3781 cagaaaagga ttaatttttt agattaattt aattgaactg ttttgagtaa aagttcagaa
     3841 gatcatataa aaagttttct tacttatata aagatcaaca gaaattattc cgataatact
     3901 gtaatctctt atgaaaatga tctgtcacag ctgattgagt atgcagaaaa atccggattc
     3961 attacagcaa aagataaaac agattttaat gatctagacc ttagtttttt aaaatcctat
     4021 gtagcggaaa tttcagatcc ttttaaaagg aaaggaaaat attcgaaaaa aagtatttca
     4081 agaaagatct ctgttataaa atcattttac aaatatctct accgcaaaaa atacatttca
     4141 aaaaattatt cgtcttcact catctttcca aagcttaaca aaaaattacc atctattctc
     4201 agcgagagtg agttaaataa attacttggt gagaaatatc tggctgaact tagtatactt
     4261 gacaaagcta ttatagaatt attctacagt accggtatca gactttctga attaataaac
     4321 ttaaaatttc ataatattga tctgactaat aaaactataa aagtaaaagg aaagggttcg
     4381 aaagaaagga tagtgccttt tggaagggaa gcagatatat ctataaaaaa ttatctgaag
     4441 atcagggata tatgtaatgt taacagtatt gataatctgt ttatggataa tgcaggtaag
     4501 atactttatc ctatgaaaat taacagaatg atcaaaaaga atttgtctct tgtcacacaa
     4561 agcagtaaga aatctcccca tgttttaaga cattcatttg ctacacatct tcttgataac
     4621 ggagcggata tccgcgctgt caaagatctt ctcggacatg aaagtctttc cactacgcag
     4681 gtttatacac acatcactcc cgaacgtttg aaaaaagttt acagacaagc tcatccaagg
     4741 tcaaaagaac tttaatataa acctacattt attcttctcc aaattatcta aaccgaatcc
     4801 gtccaatacg ttttaataca atatacagga tccatttttt ctacttactt ttttttaacg
     4861 gcaaaaaaag taacaaaaaa agtcgcccgc tggtgataac ttgccggaaa ttcttt
//
LOCUS       DAORVQ010000230         4836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12556_length_4836_cov_4.139301, whole genome shotgun sequence.
ACCESSION   DAORVQ010000230 DAORVQ010000000
VERSION     DAORVQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12556_length_4836_cov_4.139301"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="PLX80_13190"
     CDS             complement(<1..2040)
                     /locus_tag="PLX80_13190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21790.1"
                     /translation="MQIDLSKSLTKFLPVNPSEKRPLWSVMIPVYNNTSFLSQLLERV
                     LSQYKDSDQMEIFVIDDCSVSGDPESVVYKFGKGIVKYFRQPQNVGHSKNYDTCIKKA
                     KGKYIHILHQDDLIREGFYARMEKSFNDNPDIGAAFCRHIFIDEKGNWLWISELEKEE
                     DGILDNWLMKIAEKQRIQYASMVVKRKVYEDLGGFVNKETAGEGWEMAGEDWEMWVRI
                     AAKYRVGYVTEPLAEYRIHRASMTSSLAKSGQNVKDIHRIIDIFSQYIPEDKKDLVKK
                     SAAKHYANYTFENSKKILQDTDDPAAAEAQLNEALKLDPEIYSKDILLYSKLKDLIKL
                     DGISIVLTIENDADGVKDSLDEISSQLIPEGIKTEIIFVDNGSTDNTYQTAKKLISND
                     LYKGSSVKLIRQEYSDFNSALKTAFSKAEFENILVCNGRFKLSSDFLATGYKIFKEDK
                     NAALIRSSADIYTDGKLPSWINDKLLYCRFRIDNKGFRNDNDPFLINGSFIRKSVWGK
                     ILDGDIIPDLSGIVNKSVSIESELPDLLIKIGFKTVKANDLVYKKYIPIDSLKYSEFR
                     DYFENEGRISAGSRKGNIEIRRALRLFRKFNNAALQNNSSENSQDINLIYARYYSERL
                     KSLLNNRTLNKIEQTFLLRSLSRISDHKLFRNDYKQFYKEPARKGVSVIINIRDHE"
     gene            complement(2074..3027)
                     /locus_tag="PLX80_13195"
     CDS             complement(2074..3027)
                     /locus_tag="PLX80_13195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21791.1"
                     /translation="MKVSVWMSAYNHGKYISRCLDSVLMQKTNFAFEVVIGEDCSQDN
                     TRDIIIEYANKYPEIIKPFLPEKNIGMMQMDVATFPLCKGQYLALLNGDDEWTDENKL
                     QIQTDLLDSDKAISMCYHQSKVIDEITGQEWDTEFTGEGNELSVEKLFLGFNPIMTAS
                     VMCRNIGALPEWYEELPYGDMPLYLILSEMGKISYIDKVMCRYRIHAAGNWQGDSLKS
                     NLLKDLKYYNFINKKFGFRYNSFISKILALRYFSLVLLSLRNDNPAEAKHYYKSLLIS
                     DTGFLISHKKDILDLFKILYENEDKEMFNELINTDPGWKIS"
     gene            complement(3086..3784)
                     /locus_tag="PLX80_13200"
     CDS             complement(3086..3784)
                     /locus_tag="PLX80_13200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21792.1"
                     /translation="MFVKSAKFYDALYHFKDYKKASDKLTSFIRKYNPGAGTLLDTAC
                     GTGKHVEFLKNDFECEGLDINKDLLDIAKERCPEIVFHESDMTEFHTGKTYDAVCCLF
                     SSIAYVKTYENMCKAFFVMTEHLNPNGLLIIEPWFSKETYFTNTITANHYDEKDMKLA
                     WMYNSILKDDMSVLDIHYLAGTAEGVVHFNELHELGLFDDTQYRKAMTDAGLDVHYDK
                     DGLFGRGMYIGIKN"
     gene            complement(3824..>4836)
                     /locus_tag="PLX80_13205"
     CDS             complement(3824..>4836)
                     /locus_tag="PLX80_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21793.1"
                     /translation="NAKDPEMSMHSMIPADSQEWDDVIKDIRHSFAHTRESCHAMSLT
                     SGYKTFLYVFEKDGIRIVCPVAERDFNGYKDIVTPYGFSGFTGTGNHPEFKNYWKEFV
                     KEKNYVCGYISLNPLFADKDLYNTNEAFSSTNLFFLDLNKSLTELYENLDSNRKRIIK
                     NFKKEESCFIYDKSELTEFFTANYYDFLKSINASEASYFKKETLEYICDLDNVFMAGA
                     VENEKIAAVYIFAHTKYSADCLFNVPLPEGRKYAPTLLWAGLKYFMKKNIPVLNLGGG
                     LKEDDSVALSKERFGAFKLPFVNLKQIYDTDKYFSICSEVNADPKNIEGYFPAYRKPV
                     HK"
BASE COUNT         1445 a          936 c          732 g         1723 t
ORIGIN      
        1 ttcatgatca cggatattta taattacaga tacacctttt cttgcaggct ctttataaaa
       61 ttgtttgtaa tcatttctga atagtttatg atcagatatt cttgacagag atcttagtaa
      121 gaaagtctgt tcaattttat tgagagtacg gttatttaac aaagacttta atctttcaga
      181 ataataacgt gcatatatta agtttatgtc ctgtgaattt tcagaagagt tattttgaag
      241 tgctgcatta ttaaattttc tgaataatct taaagctctt ctgatctcaa tatttccttt
      301 tctactgccg gcagatatcc tgccttcatt ttcaaagtaa tctctgaact cactatactt
      361 cagactatct ataggtatat actttttata aacaagatca ttggctttta cagttttgaa
      421 tccaattttt atcagcagat ccgggagttc agattcaatc gatacggatt tattgactat
      481 gcccgataag tctggaataa tgtctccgtc taaaattttc ccccacacac tttttcttat
      541 aaatgaacca tttattaaaa aaggatcatt gtcatttctg aatcctttat tatctattct
      601 gaatctgcag tataaaagtt tatcattgat ccatgacgga agtttgccgt ctgtatatat
      661 atcagctgaa cttctgatca gcgcagcatt tttatcctct ttgaaaattt tatatccggt
      721 ggcaagaaaa tcagaactga gtttaaacct gccattacat accaaaatat tttcaaattc
      781 agccttgctg aatgcagttt ttaaagcgga attaaaatca gaatattcct gccggatcag
      841 ttttactgac gagcctttgt aaagatcgtt tgaaattaat ttctttgcag tctgataagt
      901 attatcagtt gaaccgttat ctacaaaaat tatctcagtc ttaattcctt caggtattag
      961 ctgagaactt atttcatcta aagaatcttt cactccatca gcatcatttt ctattgttaa
     1021 aacaattgat ataccgtcaa gttttatgag gtcttttaat tttgaataaa gtaaaatgtc
     1081 tttggaataa atttccggat cgagttttag agcttcgtta agctgagctt cagcagcagc
     1141 aggatcatct gtatcttgca gtattttttt ggaattttca aatgtatagt ttgcataatg
     1201 tttagcagcg gatttcttta ccagatcttt tttatcttcg ggtatatact gcgaaaatat
     1261 atcaatgatt ctgtgaatgt cttttacatt ttgccctgac tttgcaagac tgcttgtcat
     1321 tgaagccctg tgtattctgt attcagcgag cggttcggtt acataaccta ctctgtattt
     1381 tgcagcaatt cttacccaca tctcccagtc ctctcctgcc atctcccagc cttcaccggc
     1441 ggtttcctta ttcacaaacc ctcccagatc ctcatacact ttcctcttta ctaccattga
     1501 agcatattgt atcctctgct tttcagcgat cttcatcagc cagttatcca aaattccgtc
     1561 ttcttccttt tcaagttcag atatccaaag ccagtttcct ttttcatcaa taaaaatatg
     1621 ccggcaaaat gcagctccga tatcaggatt atcattaaat gacttttcca tccttgcata
     1681 aaatccttcc cttatcagat catcctgatg aagaatatga atatatttcc ctttggcttt
     1741 tttgatgcag gtatcataat tttttgaatg accaacattt tgcggctgtc tgaaatattt
     1801 tacaattcct ttgccaaatt tatatacaac actttcagga tctccgctta ctgagcagtc
     1861 gtcaattaca aatatttcca tctgatcgga atctttatat tgtgataaga ccctttcaag
     1921 aagctgactt aagaatgaag tattgttata tacagggatc atcaccgacc aaagcggacg
     1981 cttttcagac ggattaacag gtaaaaattt cgttaaggat ttgctgagat ctatctgcat
     2041 tttattatga tcgttttgaa taaattatat acatcagctt attttccatc ccggatcggt
     2101 gttgatcaat tcattaaaca tttctttatc ctcattctcg tatagaattt taaataagtc
     2161 aagtatgtct tttttatgtg aaattaaaaa tcccgtgtca ctgatcaaca gacttttata
     2221 gtaatgtttt gcttcagcag gattatcatt tcgtaagctt aataatacca gactaaaata
     2281 tctgagtgca agtatttttg aaataaaaga attatatctg aatccgaatt ttttatttat
     2341 aaaattataa tattttaaat cttttaataa attacttttc agagaatcgc cctgccagtt
     2401 gcctgcagca tggatcctgt atctgcacat tactttatca atataactta tctttcccat
     2461 ttctgaaaga ataagataaa gcggcatgtc accgtacgga agctcttcat accattccgg
     2521 aagtgccccg atattcctgc acatgacaga tgctgtcatg atcggattaa atccgagaaa
     2581 caatttttcc accgataact cattcccttc acctgtaaac tcagtgtccc attcctgtcc
     2641 tgtaatttca tcaatcactt tcgactgatg ataacacatc gaaattgctt tgtcactgtc
     2701 cagcaggtca gtctgtatct gaagtttatt ctcatcagtc cattcatcat cgccattgag
     2761 cagtgcaaga tactgacctt tgcatagtgg aaaagtagca acatccatct gcatcatccc
     2821 tatattcttt tcaggaagaa aaggttttat aatctccgga tatttattcg catactcaat
     2881 tattatatca cgggtattat cctgagaaca gtcttctcca atcacaacct caaatgcaaa
     2941 atttgttttt tgcatgagta cagaatcaag acatcgggaa atatatttcc catgattgta
     3001 agcagacatc cagacgctga ccttcatttt aattgtttaa ttgtttgaga taaattgagt
     3061 aaggattaaa ataaactaat ttttactaat ttttaattcc tatatacatg cctcttccga
     3121 aaagaccatc cttatcatag tgaacatcaa gtccggcatc tgtcatcgct ttcctgtatt
     3181 gagtgtcatc aaataatcca agttcatgaa gttcattaaa atgtacaaca ccttcagcag
     3241 ttcctgcaag ataatgaata tcaagaaccg acatatcatc ttttaatatg ctgttataca
     3301 tccatgcaag tttcatgtcc ttctcatcat aatgatttgc tgtgatcgta tttgtaaaat
     3361 aagtttcttt gctgaaccat ggctcgatga tcagaagacc gtttggattt aaatgttcag
     3421 tcataacgaa aaatgctttg cacatatttt cataagtctt tacataagct atggaactga
     3481 agagacagca gactgcatca tacgttttac ctgtgtgaaa ttccgtcatg tcgctttcat
     3541 gaaatactat ttccggacat ctttcttttg caatgtccag caggtctttg ttaatatcga
     3601 gaccttcgca ttcaaaatca ttttttaaaa actcaacgtg tttaccggtc ccgcaggctg
     3661 tatcgagaag agttccggct ccgggattat atttcctgat aaatgaagtt aacttatcag
     3721 aagctttttt ataatcttta aaatgataca atgcatcata aaattttgct gactttacaa
     3781 acaattttaa ttagatttat tttttgaaaa tttaaaattc aatttattta tgaaccggtt
     3841 ttctgtaagc agggaagtat ccctcaatgt tttttggatc cgcattgact tccgagcaga
     3901 tagaaaaata tttatctgta tcatagatct gtttaagatt tacaaaaggg agtttaaaag
     3961 ctccgaatct ttcctttgat aatgcaacgc tgtcatcttc tttcagacct ccgccgagat
     4021 tgagtacggg aatatttttt ttcataaaat acttcaatcc tgcccatagc agggtcggcg
     4081 catatttcct gccttcagga agaggtacat tgaataaaca gtccgctgaa tattttgtat
     4141 gtgcaaaaat atatacggca gcgatctttt cattttcgac agctcctgcc atgaatacat
     4201 tatcaagatc acaaatatat tcaagtgttt cctttttgaa ataagatgct tcggaagcat
     4261 taatgctttt cagaaaatca taataatttg cagtgaaaaa ttctgtcagc tcagacttgt
     4321 cataaataaa acaggattcc tcttttttga aattcttaat tatccttttc ctgtttgagt
     4381 caagattttc gtaaagttct gtcagacttt tattaagatc cagaaaaaat aaattcgtac
     4441 tgctgaatgc ttcattggta ttgtataaat ccttatcggc aaagagggga ttaagactga
     4501 tgtatccgca gacataattt ttttccttaa cgaattcctt ccagtaattt ttgaattccg
     4561 gatgatttcc tgttccggta aatcctgaaa atccataagg tgtaacaata tctttataac
     4621 cattaaaatc tctttcagca accggacaaa ctatccttat gccgtctttt tcaaaaacgt
     4681 aaagaaaagt tttgtaaccc gaagtaagac tcattgcatg acagctttct cttgtatgag
     4741 caaaagaatg tctgatgtct tttataacat cgtcccattc ctgagaatct gccgggatca
     4801 tactgtgcat actcatttca ggatcttttg catttc
//
LOCUS       DAORVQ010000231         4782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12799_length_4782_cov_6.004654, whole genome shotgun sequence.
ACCESSION   DAORVQ010000231 DAORVQ010000000
VERSION     DAORVQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12799_length_4782_cov_6.004654"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLX80_13210"
     CDS             <1..232
                     /locus_tag="PLX80_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21794.1"
                     /translation="FSSFDIHFSSFDIHSSSFNVVTSSFDVDTSSFDVDSSSFGIDLS
                     SFDVDSLSFDVDTSSFNVDFSSFDVYLSSFDN"
     gene            complement(534..1616)
                     /locus_tag="PLX80_13215"
     CDS             complement(534..1616)
                     /locus_tag="PLX80_13215"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HQY21795.1"
                     /translation="MYKKIFTLIPAIFFLIILNVNASPNPRNVLIEYVTGTWCGNCPC
                     GHQTLNTILNQHPNTIVLAYHAFSNDPFRNFNGNEIVGLMGFSSTPTTVIDRNVFIGT
                     LNYPLWTATVGDRYASSTDAPVNINIVSKTYNETTGEFTFNVEASALEDLSGQYKITA
                     VIAEDNLIHQQNFYSQCGIPGIVTNYTHNHVTRNFINGATGENLNTGGMWTGGQIISK
                     NFQTTIDASWVADNCNIVIFVNREESLLRESEVQQAITESVTGTTGISSVGSETPEGF
                     VLSQNYPNPFNPNTNIRFSIPADGNVSFNIYDISGKSVGEYVNGFLQRGSYNVEFDGS
                     GFASGIYFYELRAENFSDRKKMILLK"
     gene            complement(1909..2472)
                     /gene="msrA"
                     /locus_tag="PLX80_13220"
     CDS             complement(1909..2472)
                     /gene="msrA"
                     /locus_tag="PLX80_13220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQY21796.1"
                     /translation="MAGSSDKLELATLGGGCFWCTETIFNELKGVESVISGYSGGEIK
                     NPTYEEVCSGNSGHAEVVQITFNPAIISYEQLVEIFFHIHDPTTLNRQGADKGTQYRS
                     VIYYHNDSQHQTADEVIKKITDEKLWNDPVVTEVTKIDVFYPAEEYHQDYYENNKEKS
                     YCSIVIAPKVRKFYSQYKDLLKEDAGN"
     gene            3372..>4782
                     /locus_tag="PLX80_13225"
     CDS             3372..>4782
                     /locus_tag="PLX80_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY21797.1"
                     /translation="MKTLLTNAKVRLSGNNFVSSVGFDTETGKITFTGNPTETEKIRN
                     EYDEIIDCSKKLVMPAFYEGHCHFIEGAYVNSLLDLRDASTKKDFRERISKYRNAAGV
                     SSIEGGFFAESNFKEEINLDVNFLDSICNDVPLIISRFDTHAAFANSKAIEMSGLLSN
                     ESEYTSEEIIKENGVITGELKERARDYVLDSLPKPSLQKQTKVAKELMERFNSYGITS
                     ISDITLIPDLEVYKELLNSNSLNLNVDARLNILEFENIEKYKNAFENFYPQIKFKSFK
                     AFYDGSLSSKTAYMHENYKGTNHNGIRTEIVNSGAFSKVCKEIDNRGYQLSVHAIGDK
                     AVTDLLDFNEELIRLNGKKDRRFRIEHSQHITENDFERFRELNVIASVQPSHLFSDAK
                     TASQILEDPSLEHNYDKLFKNGTTVCFGTDFPIVNENPFETIYYAMTRKAVGFEDGFY
                     KEKGISLDDCIDAYTIRNAF"
BASE COUNT         1539 a          791 c          758 g         1694 t
ORIGIN      
        1 cttctcatct tttgacattc acttctcatc ttttgacatt cactcttctt cttttaacgt
       61 cgtcacttca tcttttgacg tcgacacttc atcttttgac gtcgactcct catcttttgg
      121 catcgacctc tcatcttttg acgtcgactc cttatctttt gacgtcgaca cttcatcttt
      181 taacgtcgat ttttcatctt ttgacgttta cctctcttct tttgacaatt aatctccttc
      241 ctcagacatt cactcttatt cttttgacag tttacattta ttactaaaaa tccatttttt
      301 caaattacta ttacatctgg aactgattgt taatttccct tcatttcata ctgatttctc
      361 cgtaaactca attcatcctt tttcaaatca tagaattttc ctctctacgt ataataaaaa
      421 aagtccatcc cttaatagtg agatggactt ttaatatctt gattattgat ttattatcaa
      481 ctgccgtaaa tttgaatatt cttatccggc aatttagcgg aatcctaaaa aatttatttc
      541 agcaggatca ttttcttcct gtcggaaaaa ttttccgcgc ggagttcgta aaaataaata
      601 ccgcttgcaa atccgctgcc gtcgaattct acgttgtaac ttcccctctg aagaaagccg
      661 ttcacatatt ccccgaccga ttttccgctg atgtcatata tattaaaact tacattcccg
      721 tctgctggaa ttgaaaatct gatatttgta ttcggattaa atggattcgg ataattctgc
      781 gagagaacaa atccttccgg cgtttccgat ccgactgaag aaattcctgt agtaccggta
      841 acactttccg taatcgcctg ctgaacttca gattctctaa gtaatgattc ttcacgattt
      901 acaaaaatca ctatattgca gttatctgcg acccaggaag catcaatagt tgtctgaaaa
      961 tttttcgaaa tgatctgccc gcctgtccac attccgccgg tattaaggtt ttcgccggtt
     1021 gcgccgttaa taaaatttct cgtgacatga ttgtgagtgt agtttgtaac aatgccgggt
     1081 atgccgcact gagagtaaaa attctgctga tggattaaat tatcttcagc aataacagct
     1141 gtaatcttat actgtccgga aagatcttca agtgctgatg cttctacatt aaatgtaaat
     1201 tctccggtag tttcgttata agttttagag acaatattta tattcaccgg agcgtcagtt
     1261 gaagaagcgt atctgtcgcc tacagttgct gtccacaatg gatagttcaa agtaccgata
     1321 aagacattcc tgtcgatcac agtagtgggt gttgaactaa accccataag tccgactatt
     1381 tcattgccgt tgaaatttct gaaaggatca ttgctgaatg catggtaagc gagaactatt
     1441 gtattcggat gctgattgag tatggtgttt aaagtctgat gaccgcaggg acaattaccg
     1501 caccatgtgc cggtgacata ttcaattaaa acattccgtg gattcgggct ggcatttaca
     1561 ttaagaatta tcagaaaaaa tattgcggga attaaggtga aaattttctt atacatataa
     1621 ttattattta gattgattta aaatttaaat actttaagct tctcaaaatt tcgtttttta
     1681 aattgttata attgtctgtt aacttaagta acgaaattaa atgagcattg tatttatttt
     1741 aaaaatataa ttttttagat gaatattcaa aagaaataaa tcaagaacct gctttgagat
     1801 ttttaacaga tattttatct gttttggttt ctgataaaat aaaacgaaaa gtccgttctg
     1861 agaactctga acggactttt taagatctga attataaaga ctttggaatt aattcccggc
     1921 gtcttctttt aaaaggtctt tatattgaga ataaaatttc ctgaccttcg gagcaattac
     1981 aattgagcag taagattttt ctttattatt ttcataataa tcctgatgat attcttctgc
     2041 aggataaaac acatctattt tggttacttc agttactaca ggatcgttcc ataatttttc
     2101 atcagtaatc ttttttatta cttcatcagc ggtctgatgc tgactatcat tatgataata
     2161 gatgacagat ctgtattgtg tacctttatc ggcaccctgt ctgttcagag tagtaggatc
     2221 atggatatga aagaaaatct ctactaattg ttcgtaactg ataattgccg gattaaaagt
     2281 aatctgcact acttctgcat gtccggaatt acctgagcag acttcttcat acgtcgggtt
     2341 ttttatttct ccgccggaat atccggagat caccgattct actcctttta attcattaaa
     2401 aattgtttct gtacaccaga aacatccgcc acctaaagtt gcaagttcca atttatctga
     2461 tgatcctgcc atgttattat tagttttata attaatttct tttacagaat ttgttgaagt
     2521 ttcagatttg ctttcctttt catttccgga taaatattta tatccgacta ttgctaatat
     2581 tataactgat aatactaaaa ttgatttttt catagtttca ttttatactt aattagttat
     2641 actcaaaatg cgttttgatt aactaataaa tcttagtatt agttcataaa ttgttttttc
     2701 gttttgtata caaaatttta aatttttgta tacaatcgct ttttattaca gttttaacat
     2761 aaagagataa ttacttcttt gatatagaaa tgtttataat tcaatattcc tcaaactcaa
     2821 taaatgtttc aaatacctca attaaacgct gttttaagca ttttctctta aacataaaaa
     2881 aacccctgcg cgaataaaac cgcacagagg taattaaagg aagaacaaat atgaaagaac
     2941 gtatcggctg gcgccgatgt atacaaacta taaaaaataa tttaataatc caatatattt
     3001 tttaatattt tttcatttta tttgaaattt cttcgtagtg tatttccatt tttactttta
     3061 aatatttcct ttaaaagttc aatcatattt atatcgattt tctttttatc cgtaaggttt
     3121 tttatttttt agaaaattac attattgtac caggcgaaac taagtttaat aaataattga
     3181 ttaaactatt tttttaattc tattagttag tgttcgaaaa ttttatttcc atactaataa
     3241 caatttaaac tcatttgatt gtatttatta tgatcaacat tatatttatt ttaaaaatac
     3301 tttctaaata aaatcatatt actgaatatt agtatcttca aaactgctaa ttttataaat
     3361 ctatcatttg aatgaaaact cttctcacta acgcaaaggt aagactttcc ggaaataatt
     3421 ttgtcagctc tgttggtttt gatactgaga caggaaagat cacattcaca ggaaacccga
     3481 ctgaaactga aaagatacga aatgaatatg atgagataat tgactgttca aaaaagcttg
     3541 tgatgccggc tttttatgag ggtcattgcc attttatcga aggtgcatat gtaaattcac
     3601 tgcttgatct aagagatgct tctacaaaaa aagattttag agaaagaatc agtaagtaca
     3661 gaaacgctgc aggagtcagt agtatagaag gcggattttt tgctgagtcg aatttcaaag
     3721 aggaaataaa tctggatgtg aattttcttg acagtatatg caatgatgtt ccattaataa
     3781 tttcaagatt tgacacacat gctgcatttg cgaattcaaa agctatcgag atgtcaggac
     3841 tattgtcaaa tgaaagtgaa tatacttcag aagagataat caaagaaaac ggtgtgatca
     3901 caggtgaatt aaaggagcgt gcacgggact atgtattaga ttctttacca aaaccctctt
     3961 tacagaaaca aactaaggtc gcaaaggaat tgatggaaag atttaattca tacggaatta
     4021 cttctatttc agatattaca cttatacctg atctggaagt atataaagaa cttttaaaca
     4081 gtaattcact gaatttaaat gttgatgcaa gattaaatat tttagagttt gaaaatattg
     4141 aaaaatataa aaatgctttt gagaattttt atccgcagat caaattcaaa tcttttaaag
     4201 cattttatga tggatcactt tcgagtaaga cagcatatat gcatgaaaat tacaaaggta
     4261 caaatcacaa tggtatccga actgaaattg tgaacagtgg tgcattcagt aaagtatgta
     4321 aagaaatcga taacagaggg tatcagcttt cagtacatgc aatcggagat aaagctgtaa
     4381 ctgatctgct tgactttaat gaagagctaa ttagattaaa tgggaaaaaa gacaggagat
     4441 tcagaataga acattcgcag catattacag aaaatgattt tgaaagattt cgggagctta
     4501 atgtgatagc ttctgttcag ccttcgcatt tattcagtga tgcaaaaact gcatcccaaa
     4561 tattagaaga tccttcactg gagcataatt atgataaact gttcaaaaac ggaacaactg
     4621 tttgctttgg aacggacttt ccgatagtaa atgaaaatcc ttttgagaca atttattatg
     4681 caatgactcg aaaggcagta ggttttgagg atgggtttta taaggaaaaa gggatttcac
     4741 tggatgactg catagatgca tatacaataa ggaatgcttt tg
//
LOCUS       DAORVQ010000232         4781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12804_length_4781_cov_4.779941, whole genome shotgun sequence.
ACCESSION   DAORVQ010000232 DAORVQ010000000
VERSION     DAORVQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_4781_cov_4.779941"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1737)
                     /locus_tag="PLX80_13230"
     CDS             complement(169..1737)
                     /locus_tag="PLX80_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQY21798.1"
                     /translation="MRFYKIILIALFCFLLNNNSISQPHTEWVQRYNSPGNYNDYVTD
                     IAIDKSGNVYLTGYTIKSTFDYDFLTIKYNNAGVFQWSKTYDGSAHEYDAPVGIDVDD
                     SGYVYIAGTSKDSITNFDYLLIKYDKFGDTVYVRKYNHSIFDEASSMCLDKQGNIFIS
                     GSGMGCDTCIPGYLTVKFNSEGVYQWSRIYKGPTKSSDFVRDIIADSIGNLYVTGQVI
                     GTMGTITIKYDQQGDTIWTRESLNGGNILKINLGNLFLMEALHRIIKYDLDGDLFWSI
                     NIEGVYYDILVNGSNSIFLPGVKSTGMSGSSAITKKLNLLGDSIWSNSFQVVSFSNNR
                     VVSIAEDKNSNLFVTGHSDYNTPWNRFLTLSYDKSGNLLWSKDYNNNNPFLQHEAVKV
                     KTDTSGNIFVAGISQGNGTGWDIALIKYSKLTGINKFSNIVPKEYKLYPNYPNPFNPL
                     TNIKYEIPLLSDIEFLIINASGKEIFKLTNSNVPPGNYNFEFNGNDIPSGIYFLRFIA
                     NNKLIKTHKLILLK"
     gene            complement(2258..2710)
                     /locus_tag="PLX80_13235"
     CDS             complement(2258..2710)
                     /locus_tag="PLX80_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21799.1"
                     /translation="MYKTTNGGRNWLIQNSTLPGVVYSLWAFNDSLVWGCGNGGRIWH
                     TIRGGDTLTNINNNSTSVIKDFKLYQNYPNPFNPTTNIKFDLPKDVQVSVKIYDMVGR
                     EIKTLAIEFKTAGRYSVTFSGADLASGVYYYKIKAGEFEQVRKMILLK"
     gene            complement(2729..3493)
                     /locus_tag="PLX80_13240"
     CDS             complement(2729..3493)
                     /locus_tag="PLX80_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21800.1"
                     /translation="MKKIILVFIFIQISYQFAEAQWFTQQSGTTNPLKDIEFINDKTG
                     WACGVDGYIIKTTNGGMNWIRHGFGITFEPLFGIHPIDSNTVYAVGFYRTIVKTTNGG
                     AEWFKAQSGMQGDGNYECVFFINTNTGWIGNFDSPEYGVRKTTNGGKSVFSIPFSGIP
                     RDLYFKDSLNGIGVGGLSYIYKTSDGGLNWNSFSLVLTGDFYRVSFINDNTGFTASRR
                     AVYKTTNFGTDWDSIGNIPVALGSVLSVEFCNENTG"
     gene            complement(3553..>4781)
                     /locus_tag="PLX80_13245"
     CDS             complement(3553..>4781)
                     /locus_tag="PLX80_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21801.1"
                     /translation="LRMKKTLLVLFLFLISIQNSEAQWFTQQSGTTNPLYDIEFINEQ
                     TGWCSGVGVILKTTNGGVHWIDFLNDAPSKPYSAIFPVDTNVVYAVGYFRTFIKSTNG
                     GVNWTIIENGNTGEGDYYSLFFLNQNTGWAGVNYANGIRGVRYTTDGGTNFIIGQTGI
                     PQDLYFKDSLNGIGVGGLSYIYKTTDGGLNWNSFSLVFTGDFYRVSFINDYTGYTASK
                     RAVYKTTNFGSDWDSVGNIPVSLGSVLSVEFCNENTGWAGTQYQIFKTTNGGRDWFSQ
                     IGTGVIYNITSFNDSLVWACGNGGRIWHTMNGGTSNLNNISNSIPSIFKLYQNYPNPF
                     NSKTKIRIDIRKTGNYEMEIFNNTGKKIETFFNVELNTGQYEIIYDAKELSSGIYFYR
                     LSGNNISETKKLILIK"
BASE COUNT         1509 a          865 c          674 g         1733 t
ORIGIN      
        1 gataaaaaaa gagggcaaga cagaatttac aaatttgaat aatttgtaat tatcttttat
       61 taataccctg cacggattaa tcgaagaaag tctctgccct ctttttaaat tttttcaggt
      121 cgctttaatc gaaccagttt ttcaaactga ttcataaaac ctcccttttt attttaacaa
      181 aattagttta tgtgttttta taagtttatt atttgcgata aatctaagga aatatatccc
      241 gcttggtata tcgttaccgt taaactcaaa attgtaattt ccaggtggaa cattggaatt
      301 tgtcagttta aatatctctt taccggaagc attaataatt aaaaattcaa tatctgataa
      361 taaagggatt tcatatttga tattcgtcaa cggattaaag ggattaggat aattcggata
      421 aagtttgtat tcctttggta caatatttga aaacttattg attccggtta actttgaata
      481 tttaattaat gcaatatccc aacctgttcc gtttccctgg ctgatacctg caacgaaaat
      541 atttccggag gtgtcagttt ttacttttac cgcttcatgt tgaagaaacg ggttattgtt
      601 attataatct tttgaccata ataaatttcc tgatttatca tatgaaagag ttaaaaatct
      661 gttccatgga gtgttataat ctgagtgacc tgtgacaaat aggttagaat tcttatcctc
      721 ggctatcgat accactctat tattactaaa ggagactact tgaaaactat tactccatat
      781 cgaatctcct aagagattaa gcttttttgt aatagctgat gacccagaca ttcctgtact
      841 ttttacacct ggaagaaaaa ttgagttaga accattaaca aggatatcat aatatactcc
      901 ctcaatattt atagaccaga ataaatcacc atctaaatca tatttaataa ttctatgaag
      961 agcttccatt aaaaaaagat ttcccaaatt tattttaaga atattacctc cgtttaaaga
     1021 ttctctcgtc cagatggtgt caccttgctg atcatatttt attgtaatag ttcccattgt
     1081 tccaatcacc tgccccgtaa cgtaaagatt tcctattgaa tcagctataa tatctcttac
     1141 aaaatcagaa ctttttgttg gacctttata aatccttgac cattgataaa ctccctccga
     1201 gttaaattta acagttaaat atcccggtat acaagtatcg cagcccatac cggatcctga
     1261 aataaatata tttccttgtt tatctaaaca catggaagat gcttcatcaa atatagagtg
     1321 attgtatttt ctcacatata ctgtatcacc aaatttgtcg tattttataa gtaagtaatc
     1381 aaaatttgta atactgtctt tgctcgtacc agcaatataa acataacctg aatcatcaac
     1441 atcaatgcca acaggtgcgt catattcatg agcagaacca tcataggtct tagaccattg
     1501 aaagactcct gcgttattat acttaatagt gaggaaatca taatcaaatg tactttttat
     1561 tgtatatcca gtcaaataaa cattacctga tttgtcaatt gcgatgtcag ttacatagtc
     1621 gttataattt ccaggactgt tatatctctg cacccactca gtatgcggct gagagataga
     1681 attattatta agcaaaaagc aaaacagcgc tattaaaatt attttataaa atctcatatt
     1741 gaatttattt atattgtata ttctgaaatt tatatatcgg ttgatttttc atgcttaatc
     1801 cttttaggta aatcattttt aattataaaa atatataaag acaacttata ttataccgta
     1861 tgactttcta atgaaattta tcaccgtttc tgatcattgt taccgcagca acgggctttc
     1921 aaacaatttt gtctgactga caggagctaa aattgaattt aaatttaaga atagaattaa
     1981 ataaagtttt tcatattagc ttcactaaag tttttcatat ttttgtttaa gatattttct
     2041 tttggggcgg gaatatcttt tttcgttttg catataatta tgaaaaaaaa aaaaaaaaat
     2101 caagttaatt tattttgaat tttctaatca gggagattgg cgcggctgtg tgcggaagtt
     2161 acatttaaaa aaaatcccgc aaagattttt acctctgcgg gataaagtag tatttgtaaa
     2221 attatatatt tttaatttga attttgtaat ttgaaattta tttgagtaga atcatcttcc
     2281 tcacttgttc aaattcccct gcttttattt tatagtaata aactccgctt gcgagatcag
     2341 ctccgctgaa tgttacggaa tacctgccgg ctgttttgaa ttcgattgca agtgtcttta
     2401 tttctcttcc gaccatatca tatattttta cagaaacctg aacatctttc ggcagatcga
     2461 attttatgtt tgtagttgga ttgaatggat ttggataatt ttgatatagc ttaaaatctt
     2521 taataacaga agttgaatta ttgtttatat ttgttaaagt atcaccgcct ctgattgtat
     2581 gccaaattct acctccgttt ccacatcccc aaactaatga gtcgttaaat gcccacaaac
     2641 tatataccac tccaggtaaa gtagaatttt gtattaacca gtttcttccg ccattcgttg
     2701 tcttatacaa ttgatattga gtaccggctc aacctgtatt ttcattacaa aattctacgc
     2761 ttaaaacact tccaagagct accggaatat ttcctatgct gtcccagtct gtaccgaaat
     2821 ttgttgtttt gtaaactgct ctacggcttg cagtaaatcc ggtattatca tttatgaaag
     2881 atactctgta aaagtcacct gtaagtacta acgaaaaaga attccagttt aatccgccgt
     2941 ctgaagtttt gtatatatat gataatccgc ctacaccaat tccgttgaga ctgtctttaa
     3001 aatacaaatc ccttggtatg ccgctaaatg gtatagaaaa tactgattta cctccatttg
     3061 ttgtttttct tacaccatat tccggactgt caaaatttcc aatccagccc gtatttgtat
     3121 ttatgaaaaa cacacattca taatttccat ctccctgcat tccgctttga gccttaaacc
     3181 attctgctcc tccgtttgtt gtcttgacaa ttgttctgta aaaccctaca gcataaacag
     3241 tatttgaatc aatgggatgt attccgaaaa gcggttcgaa tgtaattcca aatccatgcc
     3301 ttatccaatt cattcctccg tttgttgttt ttataatgta tccgtcaact ccgcaagccc
     3361 atccggtttt atcatttata aactcgatat ctttaagagg attagtagtt ccgctctgct
     3421 gagtgaacca ttgagcttct gcaaattgat aagagatttg aataaaaata aatactaaaa
     3481 ttattttttt cattattttt aatttaaaaa ttaaatgaat ttaaaaatgt ctgtctgcaa
     3541 atcacttatt ggctatttta tcaatataag cttttttgtt tcactgatgt tatttcctga
     3601 caatctgtaa aaatatattc cggagcttaa ttctttagcg tcatatataa tctcatactg
     3661 acctgtattt aattcaacat taaaaaatgt ttcaattttt ttacctgtat tattaaatat
     3721 ttccatttca taattacctg tttttctaat atcaattctt atttttgtct tactgttaaa
     3781 tgggtttgga tagttttgat ataatttaaa aattgaagga attgaatttg aaatgttatt
     3841 taaattactt gtgcctccgt tcattgtatg ccagattctt cctccgtttc cgcatgccca
     3901 gaccaagctg tcgttgaaag aagtaatgtt gtatattaca cccgtgccga tttgcgaaaa
     3961 ccaatctctt cctccgtttg tagttttaaa aatctgatat tgcgttccag cccaaccagt
     4021 attttcatta caaaattcta cacttaaaac acttccaaga gataccggaa tatttcctac
     4081 gctgtcccag tctgaaccga aatttgttgt tttataaacc gctcttttac tggctgtata
     4141 ccctgtataa tcatttatga aagataccct gtaaaagtca ccggtaaaca ctaatgaaaa
     4201 tgaattccag tttaatccgc cgtctgtagt tttataaatg taagataatc cgcctacgcc
     4261 aattccgtta agactgtctt taaaatataa atcctgaggt atgccggtct gacctattat
     4321 aaaattagtg cctccgtcgg tagtataacg aactcccctt attccatttg cgtaattaac
     4381 acccgcccaa cctgtatttt gatttaaaaa aaataatgaa tagtaatctc cctctcctgt
     4441 attcccattt tcaattattg tccaattcac accgccattt gttgatttta taaatgttct
     4501 gaaatatcca actgcataaa ctacatttgt gtctacaggg aatatagcag aataaggttt
     4561 tgacggagca tcatttaaaa aatcaatcca gtgtacccct ccgtttgttg tttttaaaat
     4621 aactccaact ccgctgcacc atcctgtctg ttcatttata aattcaatat catacaatgg
     4681 attcgttgtc ccgctctgct gagtgaacca ttgagcttct gaattttgaa ttgaaattaa
     4741 aaataagaat agaactaata aagttttttt catacgtaaa c
//
LOCUS       DAORVQ010000233         4757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12923_length_4757_cov_5.738197, whole genome shotgun sequence.
ACCESSION   DAORVQ010000233 DAORVQ010000000
VERSION     DAORVQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12923_length_4757_cov_5.738197"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..3420
                     /locus_tag="PLX80_13250"
     CDS             130..3420
                     /locus_tag="PLX80_13250"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21802.1"
                     /translation="MSKKIIAFSVLCLFGIFMFTESANSQGFNSVSTPDGINVIAVGN
                     SGQLYRSASGGSVWVSNPNGALNMNDVTSLGNDVWIAANNSTVYKTQKTNSAVVPYLV
                     GVSVNLYSIDFVDANTGFVCGQSGVVRKTINGGVNWTSSNSGISSEDLNGIDFSDANN
                     GRVVGNNGVNYVTSDGGASWTSQSSGTTYNLTDVKLFGSFAVATGENGTLLENSSGTW
                     NAVLTSTISDFTGVSGTASNDVHVSGGGGFIRNNKSGSSNYFNFEINPMMANLVDIFY
                     YDANNGWAVSSLNNVIIYTSNAGATWSMPTGASLSRSWTQKLTASGGIGNNLCMHPYN
                     NKTIFLVQGSTVYVSRNSGENWSNIASVSGGGSAHSFYVSPLDTNVWVCAITSSPDRI
                     TKTTNYGATWTTSISANFSNYGQPLEMDQNNPNVYYFAPDNDGFYKSTDGGSSFTEIS
                     TTITGGQPGPSFRSPCDIIVKWGNSNIIMIGDGVTGSGQAQFFQSTDGGKVWDAKLSA
                     AASEIPSMCNSAFDDELFFGTEWGGSLIYKSTNSGANWASYFNTGFSGWASGFCSEDP
                     TVVYTGNYGSNSSLSTNGGSNWLIGASGMSGAGAGVIVPERGVIISQQTSNVYKMNFN
                     YSYTPTVEQIDVEVLSIGDQGTVYYPTLTITPSGTVANNNGGGPASFTVTRKITPGSY
                     TSTKNINIGAASSTSVNFDPWTFNTGTVYTIKDSVHISGDGNTSNDVMTGTLTPIVGT
                     FSISMQQDFSSGTFPPSGWTRTGSGTQFWKRDANVTGYGTGGGSSFYDFFSANAGTAY
                     QYINGPTFTPIVGTGNIQYDYAYAPYSSNDRDSITVQYSTNAGSTWLTLVQLFGYDGA
                     TGDYAMNTTGVDNNEFIPESNEWATKQWTLPAGTDRVRFRAKSGFGNNFYLDNIKILT
                     GQAYTQMNIILAPEAMYNGATLNISDTLIAYLRNNSSPYNLVDSSTAVLNGKSLTASC
                     VFQNVTTGTYYIQIEHRNTLNSWSQTGGHAVTMGMTSTYDFTTSQSKTYGSNSVLVAS
                     KYCIYSGDVNKDGIIDLADQSDIDNDGNNFVTGYVVTDLNGDGLVDLADAVTADNNAA
                     NFISAVTP"
     gene            3574..4737
                     /locus_tag="PLX80_13255"
     CDS             3574..4737
                     /locus_tag="PLX80_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21803.1"
                     /translation="MLINCFNIKITFLSILLFTVFIQISAAQSNEEKILRGIDHVYHL
                     RFDSAEVEFNQVILNDPSNPSGYFFLTMVDWWKINIDRENEFLDDEFEKRVDKVIEVC
                     DVKLDKNENDDMAMFYKGGALGYRGLVNSIRESWLKAAEDGKEALNLLQQAHELNKNN
                     KEVIFGVGLYNYFADYIPERYPVVKPLMIIFPKGDKLKGLAQIKETSVNSVYAKTEAK
                     FVLAYLNLIYEKNYLETELYSLPLHEEYPENPTFEKYLFNSYAGLGKWNEALSGWKSV
                     TEKCDSGVKGYTGKFLKRESNYYTALSYAKLNRLLESEQYINTAEEITKEIDKDNEHA
                     FTAFIYLLQGMLNDVKGNRSTAILYYDKVLVMKDFQDSHIQAEKLKQESFKQF"
BASE COUNT         1600 a          735 c          958 g         1464 t
ORIGIN      
        1 gggagctttt catttctaaa agttttgtat acaaaatttc ataaaaatca gtttataatt
       61 gaaattcaaa ttcatttgta gtattttaaa ccactcaaaa ttttttataa atcaaaaatc
      121 aattaaaaca tgagcaagaa aattattgct ttctctgttc tgtgcctttt tggaattttt
      181 atgttcacag aatcggcgaa ctctcaggga ttcaacagtg tctctactcc tgacggaatc
      241 aatgtaatag ctgtcggtaa ctcaggtcag ttatacagat cagcatcagg aggtagtgtc
      301 tgggtttcaa atccaaacgg agctttaaac atgaacgatg ttacatcttt aggaaatgac
      361 gtatggatag cagcgaataa cagtacagtt tataaaactc aaaaaacaaa ttctgcagtt
      421 gtaccttatt tagtaggtgt ttcagttaat ctttattcaa tagattttgt agatgcaaat
      481 actggttttg tgtgcggtca gtcaggcgtt gtaagaaaaa ctattaatgg cggagtgaac
      541 tggacttcaa gtaattcagg tattagttca gaagatctta acggaataga tttcagtgat
      601 gcaaataacg gaagagtagt aggaaataac ggtgtaaatt atgttacctc tgacggaggt
      661 gcaagctgga cttcacaaag ttcaggaaca acatataatt taactgatgt aaaattattc
      721 ggatcatttg cagttgcaac cggtgaaaac ggtactttat tagagaacag cagcggaaca
      781 tggaatgcag ttttaactag tacaatttca gattttacag gtgtcagcgg aacggccagc
      841 aatgatgttc atgtatccgg cggtggcgga tttatcagaa ataataaatc aggcagcagt
      901 aattatttca actttgaaat aaatccaatg atggcaaatc ttgtggatat attttattat
      961 gatgcaaata acggatgggc agtcagcagt ttaaataatg taatcattta tacttctaat
     1021 gcgggagcga cctggtcaat gcctacagga gcatcattat ccagaagctg gacccagaaa
     1081 ctaacagcaa gcggaggtat tggtaataat ctttgtatgc atccttataa taataaaact
     1141 atctttctgg tacagggaag tacagtatat gtatccagaa acagtggaga gaactggtca
     1201 aacatcgctt cagtttcagg tggcggttcg gcacattcat tttatgtaag tcctcttgat
     1261 actaatgtct gggtatgtgc aattacttcg agtcctgaca gaataacaaa gacaacaaat
     1321 tacggagcta cctggactac cagtattagt gcaaatttca gtaattatgg acagccgctt
     1381 gagatggatc agaataatcc gaatgtatat tattttgcac cggataatga cggattttac
     1441 aaatcaactg acggagggtc ttcttttact gagataagta caactataac cggaggtcag
     1501 ccgggacctt ctttcagaag tccatgtgat attattgtta aatggggaaa ttctaatata
     1561 ataatgatag gtgacggagt tacaggtagt ggtcaggcac agttctttca gtcaactgac
     1621 ggtggaaaag tatgggatgc aaaactttca gctgcagcta gtgaaattcc atcaatgtgt
     1681 aattctgcct ttgatgatga actttttttc ggaactgaat ggggaggttc tttaatttat
     1741 aaatccacaa attcaggtgc taactgggct tcttatttca ataccggatt cagtggctgg
     1801 gcatcaggat tttgttcaga agatccgaca gtagtatata ctggaaatta cggatcaaat
     1861 tcatctctat ctacaaatgg cggatcaaac tggcttatcg gagcttcagg aatgtcaggt
     1921 gctggtgcag gtgtgatcgt tcctgaaaga ggagttataa tttctcaaca gacaagtaat
     1981 gtttataaaa tgaattttaa ttattcatat acaccaaccg tcgaacagat tgacgttgaa
     2041 gttttatcaa taggcgatca aggaacagtt tattatccaa ctttaactat tactccaagc
     2101 ggaacagttg caaataataa cggcggaggt ccggcatctt ttactgtaac aagaaagatc
     2161 acacccggaa gttataccag tacaaaaaat attaatattg gtgcagcaag ttctacttct
     2221 gttaattttg atccatggac attcaatacc ggaacagttt atactataaa ggattcggtt
     2281 catatatcag gtgatggtaa cacttccaat gatgtaatga caggaactct gactcctatt
     2341 gtgggtacat tttcaatttc tatgcagcag gatttttcat cgggtacatt ccctccgtca
     2401 ggatggacaa gaactggaag cggtacacag ttctggaaaa gagatgcaaa tgtaaccgga
     2461 tacggaacag gcggcggatc atctttttat gatttcttta gtgccaatgc tggtacagct
     2521 tatcagtata ttaacggacc gacatttact cctatagtcg gtacaggaaa tattcaatat
     2581 gattatgctt atgcacctta cagctctaat gacagagatt caataactgt tcaatattca
     2641 acaaatgcag gttctacttg gcttacatta gttcagttgt ttggttacga cggagcaaca
     2701 ggagattatg caatgaatac cacgggagta gacaataatg aatttattcc tgaatcaaat
     2761 gaatgggcaa ctaagcagtg gactcttcct gctggaacag acagagtgag atttagagct
     2821 aagagtggtt ttggaaataa tttttatctg gacaacataa aaattctgac aggtcaggca
     2881 tatacgcaga tgaatattat tttagcacct gaagcaatgt ataatggagc gacattaaat
     2941 atttccgata cactgattgc atatttgaga aataattcaa gtccgtacaa tctcgtagat
     3001 tcatctacag cagttttaaa cggaaaaagt cttacagctt catgtgtatt tcagaatgta
     3061 accacaggaa catactatat tcagatcgaa catagaaata cattaaattc ctggagtcag
     3121 accggcggac atgctgtcac tatgggaatg acatctactt atgattttac aacttcacag
     3181 agtaaaacat acggaagtaa ttcagtactt gtcgcgtcaa aatactgtat atacagtggt
     3241 gatgtaaata aagatggaat tattgattta gccgatcaat ctgacattga taatgatgga
     3301 aataattttg ttaccggcta tgtagttaca gatcttaacg gagacggatt agtagatctg
     3361 gctgatgcag ttactgctga caataatgca gccaacttta tttctgcagt tactccataa
     3421 acaaattatt aaaaatattc ggaacttttt ttatagttaa gaattaaata gttcagaaat
     3481 aaaaaagaca gtttatttta taagctgtct ttttttatct gtatttttaa aaagctattt
     3541 taatactatt ttgataatta attattgtaa taaatgctga taaattgttt taatataaaa
     3601 attactttcc tctcaatttt attattcact gtttttattc agatatctgc tgctcaatca
     3661 aatgaagaaa aaatattaag gggaatagac catgtatatc atctcaggtt tgattcggct
     3721 gaagttgaat ttaatcaggt aattttaaat gatccgtcaa atccttcagg ttattttttt
     3781 cttacaatgg tggactggtg gaaaataaat atagacaggg aaaatgaatt tcttgatgat
     3841 gagtttgaaa aaagagtaga taaagtaata gaagtatgtg atgtaaagct tgataaaaat
     3901 gagaatgatg atatggcaat gttttataaa ggaggagctc ttggttacag aggtctggta
     3961 aacagtatca gggaaagctg gctcaaagcg gcggaagatg gtaaggaagc acttaatctt
     4021 cttcagcagg cacatgaact taataaaaat aacaaagaag ttatattcgg agtcggtctg
     4081 tataattatt tcgctgatta cattcccgaa agatatcctg ttgtaaaacc acttatgata
     4141 attttcccta aaggtgataa gttaaaaggt ctggcacaga tcaaagagac ttcagtaaat
     4201 tcagtctatg caaagactga agcaaaattt gttcttgcat atctgaatct catttatgaa
     4261 aaaaattatc tggagactga gttatactct ttaccgcttc atgaggaata tcccgagaat
     4321 ccgacttttg agaaatattt atttaacagc tatgccggtc tggggaaatg gaatgaagca
     4381 ctgtcgggat ggaaaagtgt taccgaaaaa tgtgatagcg gagtcaaagg atatacaggt
     4441 aaattcctga aaagagaatc aaattattat actgctttaa gttatgccaa gctgaacagg
     4501 ttactcgagt cggaacaata tataaatact gctgaagaaa ttacaaaaga aattgacaag
     4561 gataatgaac atgcatttac tgcttttatt tatcttcttc agggaatgct gaatgatgta
     4621 aaaggaaaca ggtcaactgc tattttatat tatgacaaag tacttgtaat gaaggatttt
     4681 caggattcgc atattcaagc ggaaaagttg aagcaggaga gttttaaaca gttctaagaa
     4741 attttgtttt atataaa
//
LOCUS       DAORVQ010000234         4751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_12953_length_4751_cov_9.306644, whole genome shotgun sequence.
ACCESSION   DAORVQ010000234 DAORVQ010000000
VERSION     DAORVQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12953_length_4751_cov_9.306644"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLX80_13260"
     CDS             complement(<1..1013)
                     /locus_tag="PLX80_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21804.1"
                     /translation="MATEVSLRKISSGLITFYSTYSDASDNAEEYDVISIYADLDEQL
                     ILKNLVDIYIDPGTVINYSGAEPTIYDKDVAVCNITGGGIIKNSYSGTSKVECIAVYN
                     SGSRVNIECLSIEGTGDNSSSVGGACVNVEAAERFSLKCKSVSNNYNTAIIISDCNDY
                     FINIQEVESGKPNTPNAGASVISIDGSGSVYINSLTCTGYGSCLKHSGGNLAATIYNI
                     NTLLPANETPPTAEPAIIVEEGNSPPELVLYFNEIKNYNSNDGDAVKVTGGNATLIGN
                     NIYCVNGLSLDLTDDIQSAYIKSDNIISLTKGINIDNENDPIVIEANYIEGSNGNDGV
                     VRSA"
     gene            complement(1065..1367)
                     /locus_tag="PLX80_13265"
     CDS             complement(1065..1367)
                     /locus_tag="PLX80_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21805.1"
                     /translation="MSAIILKDKVTGDESFYDTFNEAINQADKGDLIRITKNFDDQNE
                     EQFDYQLEITKDVDIIFEEGVKFKRKKDSPPLIIVNENCKELITCNISGLNIEIVL"
     gene            1606..1950
                     /locus_tag="PLX80_13270"
     CDS             1606..1950
                     /locus_tag="PLX80_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21806.1"
                     /translation="MTYEDLLTGLIESDDRFMVLTAENRATIRSLPGKIGDKFLDTGI
                     TEQTLVGAACGLALRGRIPVAHALATFHTMRAFEFIRTDVGIGNLPVKLVGGFSGFLS
                     EANGPTHPPLCL"
     gene            2363..>4751
                     /locus_tag="PLX80_13275"
     CDS             2363..>4751
                     /locus_tag="PLX80_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21807.1"
                     /translation="MKKLIFTICMLLFIIIVPLEINAQWVQVNIGTVNVSTLKSDGNL
                     LLAGTNSGFYLSSDNGATWNQRNNGLTNLGIRDLAVSGTNYIVATDSGEYLSSDQGAN
                     WNFFGITPFPLHNFAVIGSTIMASSGPSVFRSTNFGNNWSLEHFFLLFNAYGEPSNIT
                     CIEAIGSTFHLGSYDGGAVLSTDYGSNWVWDGGGLPNHYPLSLPTVYGFASIGNNLYL
                     GASGFTFNGIYTGGLYHRDINATGWSLLSNNLYMSITCLGSESDKLFVGASGFLMTTN
                     NGLNWINKSQGLNPSGNIYSILNKDDLIFIINNGRIWKRNKAEMYSYQPNDIGAVKVS
                     NPQNNGNYYVDCDSGVYIYPGTTIENFGSNNQITPFEVFIEIKYGTNTVYLNSKFDTI
                     SAGGSHDVIFDPYFVPENAVGEFKIKSWTKLLSDSNEYNDTSWSVFNVFNPNYSVTPN
                     VLSGGYEFANSTTGAGCAPDQPVFYWEDTTGSISLISNGIPQIPLFEGDIDNGVFLID
                     NIFPSGINFRFFSDVFNDFKISTNGIIGMGENSVGITDPDPKPVPELSTMVVPAFFAF
                     WCDLDFSDPDVMGRNLKYKVNNDRLIITYDRVPVKNPLQDSEDYLSFQIILEPNPTSN
                     GILTVQFDNTKTGSTFLNKYYSNTLNAHTIGLQEYDYRINRGIQYRHQNSAGVVTTNG
                     PLFSSPLAVSFGSDNSVLPAELLSFTSSVNENNVNLIWQTNKELNNSGFDIERSAIDD
                     QWIKAGFIPGNGNSNVPKEYSFTDKNLAPGKYKYRLKQIDFNGSFEYYSLAGEVSI"
BASE COUNT         1483 a          811 c          823 g         1634 t
ORIGIN      
        1 gcagacctta ccactccgtc atttccattg cttccttcta tataatttgc ttctattact
       61 atcggatcat tttcgttatc tatatttatc ccttttgtaa gactgattat attatcactt
      121 tttatataag ctgattgtat atcatctgtc agatcgagag ataatccgtt tacacaatag
      181 atattatttc ctatgagagt tgcatttccg ccggtgactt ttacagcgtc accatcattt
      241 gaattgtaat tttttatttc attaaaatat aacacaagct caggcggact gtttccttcc
      301 tcgacaatta ttgcaggctc agcagtgggt ggagtttcat ttgccggaag taatgtgttg
      361 atattataaa ttgtagctgc aagatttcct cctgaatgtt tcagacatga tccgtatccg
      421 gtacatgtca gagaattaat ataaacagaa cctgatccgt ctattgaaat tacagatgct
      481 ccggcattgg gagtatttgg ttttcctgat tcgacttcct gtatatttat aaaataatca
      541 ttacagtcgg aaatgatgat cgcagtatta taattatttg aaacgctttt acattttaag
      601 ctgaatcttt ctgcagcttc gacatttaca catgcaccgc cgaccgaact actgttgtca
      661 cctgtacctt ctatacttaa acattcaatg tttactcttg acccgctgtt ataaacggca
      721 atacattcta ccttagatgt tccggaatac gaatttttta taatgccgcc ccctgtaata
      781 ttacatactg caacatcttt gtcatatata gtaggttcag cgcctgagta attaataaca
      841 gtacccggat ctatgtatat gtcaactagg ttttttaaaa taagctgttc gtcaagatct
      901 gcataaatac tgatcacgtc atattcttca gcattatcac ttgcatctga ataagtagaa
      961 taaaatgtaa ttagtcctga actaattttc cggagggata cttctgttgc catagtggaa
     1021 ggtattaaaa ttaaaaattc attcttataa ttttcctaat attttcataa aactatctca
     1081 atatttaatc ctgatatatt gcatgttatc agttccttac aattttcatt tacaatgatc
     1141 agaggaggag aatctttctt ccttttaaat ttaactcctt cttcaaaaat aatatcaacg
     1201 tcttttgtga tctctaattg ataatcaaac tgttcttcat tctgatcatc aaaatttttc
     1261 gtaattctga tcagatcacc tttgtcagct tgatttatgg cttcattgaa tgtgtcataa
     1321 aaactttcat cacctgtgac tttgtccttt aaaattattg cagacatttt ttatctgaaa
     1381 ttaatttagg aattaaattt ttctgtaaca atgtaaaaca ttttcgaaaa aaaaatgcat
     1441 aaaaatcctt tatctgttta gagtttataa attacaagct ttacatttat ttatgattta
     1501 ttaaaattaa ttattataca ttgaaaattt gtaattgaac attgttttct atattgtttg
     1561 ctttactcaa ttttaatagc tagctgaaac cattttacat ttaaaatgac atacgaagat
     1621 ttattgacag gacttatcga aagtgatgac aggtttatgg tgctgacagc tgagaacaga
     1681 gcgactataa gaagtttacc gggaaagatc ggagataaat ttctcgatac gggaattaca
     1741 gagcagacgc ttgtcggcgc agcatgcgga cttgcactga gaggcaggat tcctgttgcg
     1801 catgcactgg caacgtttca tactatgaga gcttttgaat ttatccgcac agatgtgggc
     1861 attggaaatc tccctgtaaa gttagtcggc ggattttcag gatttctttc ggaggcaaac
     1921 ggtccgacac atcccccctt atgcctctaa gtaaaattct atttacttaa aattacagtt
     1981 taattagttt tcaattgaca gtgatcagaa ttgcgcagtt aagatcagat gtatttaaaa
     2041 gaactataga aaattaacgg gagttggctt taaaaaacaa tggacatttt gacgatttta
     2101 cagagtgaat ttttttcgga ttttggaaga tttattttta tattgcagtg attttttttt
     2161 agtttagcgt gtaaactatc attgcaaaaa gtatacctat tatcacaata ctcctacaca
     2221 atttttatga tttttatcat taaacattct gaccacagtc ctgttctctg aaaattattc
     2281 ctggtaatat tcagacgata aatttagtca aacttctgat attattgaga tgaactaatt
     2341 ttttaattaa aatttagata tcatgaaaaa gttaattttt acaatttgta tgctgttatt
     2401 tattataatt gttccgcttg aaataaatgc gcaatgggtt caggtgaata taggaactgt
     2461 aaatgtttca actcttaaaa gtgatggaaa tttgttactt gccggtacga attccggttt
     2521 ttatttgtcc tcagacaatg gagctacctg gaatcaaaga aataatggat taacaaattt
     2581 agggattcgg gatctggcag ttagtggtac aaattatata gttgctactg atagcgggga
     2641 atatctttct tctgatcagg gtgctaattg gaattttttt gggattaccc cattcccgct
     2701 acataatttt gccgttatag gatctacaat tatggcaagc agcggtccaa gtgttttccg
     2761 atctactaat tttggaaata attggagttt ggaacatttt ttcctcttgt ttaatgcata
     2821 tggggaacct tcaaacatta cttgcataga ggcaattggt tcaacatttc acctgggatc
     2881 ttatgatggc ggtgctgttt tgtcaactga ttatggctca aattgggtat gggatggcgg
     2941 cggtctaccc aatcattatc cgttatcgct tcccacagtt tatggatttg catccatcgg
     3001 taataattta tatttaggag catccggatt tacctttaat gggatttaca cgggtggttt
     3061 ataccacagg gatatcaatg ctacgggctg gtcactacta agtaataatt tatatatgtc
     3121 aatcacttgc ctgggatcag aatctgacaa attgtttgtt ggtgcatcag gatttcttat
     3181 gacaactaac aatggattaa actggataaa taaatctcag ggattaaatc cttccggtaa
     3241 tatttattcc attcttaata aggatgatct tatttttatt atcaataacg gcagaatctg
     3301 gaaacgcaat aaagcggaaa tgtattccta tcaaccaaat gatattggtg cggttaaggt
     3361 ttcgaatcct cagaacaatg gtaattatta tgttgattgc gattccggag tttatattta
     3421 tccaggaaca acaatagaaa acttcgggtc aaataatcag ataacacctt ttgaagtatt
     3481 tatcgaaatc aagtacggaa caaacactgt atatctgaac tccaaatttg acactataag
     3541 tgcgggtggc tctcatgatg taatattcga tccttatttt gttcctgaga atgcagtggg
     3601 ggaatttaaa ataaaatcat ggactaaatt gctgtcggat tcaaatgaat ataatgatac
     3661 atcctggtcg gtatttaatg tatttaatcc aaattattca gttacaccaa atgtactttc
     3721 aggaggatat gaatttgcaa attcaacaac tggtgccggt tgcgctcctg atcaacctgt
     3781 tttttattgg gaagacacaa caggaagtat aagtcttatt tcaaatggaa tacctcaaat
     3841 ccctttattt gaaggagata tagataacgg ggttttttta atagataaca tttttccatc
     3901 cggtattaac ttcagatttt tctctgatgt ttttaatgat ttcaaaattt cgacaaatgg
     3961 aattataggt atgggggaaa attcagtggg aataacagat cccgacccga aaccggtacc
     4021 cgaacttagt actatggtag tgccggcatt ttttgcattc tggtgtgacc ttgattttag
     4081 tgatcctgat gtaatgggaa gaaatttaaa atataaagta aataatgaca ggctgatcat
     4141 tacttatgac agagttcctg tcaagaaccc attacaagat tctgaagatt atttatcctt
     4201 tcagataatt ctggaaccta atccgaccag taatggaatt ctaacagttc agtttgacaa
     4261 tacaaaaacc ggttcaacat tcttaaacaa atattattcc aatacattaa atgcacatac
     4321 tatcggactc caggaatatg attacagaat caatagagga attcaataca gacaccaaaa
     4381 ctcagcggga gtggtaacaa caaatggtcc tttattcagt tcaccacttg cagtttcgtt
     4441 tggttctgat aattcagttc tcccggctga acttttatct ttcacttcat ccgttaatga
     4501 aaacaatgtc aatttaatct ggcagacaaa caaagaatta aacaactccg gctttgacat
     4561 tgaaagatca gcaatagacg accagtggat aaaggcaggt ttcattcccg gaaacggaaa
     4621 ttcaaatgta ccaaaagagt attcattcac agacaaaaac cttgctcccg gaaaatacaa
     4681 atacagatta aagcagatcg actttaacgg cagctttgaa tattacagtc ttgcaggtga
     4741 agtgtctatc g
//
LOCUS       DAORVQ010000235         4725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_13087_length_4725_cov_5.452677, whole genome shotgun sequence.
ACCESSION   DAORVQ010000235 DAORVQ010000000
VERSION     DAORVQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13087_length_4725_cov_5.452677"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1520
                     /locus_tag="PLX80_13280"
     CDS             150..1520
                     /locus_tag="PLX80_13280"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQY21808.1"
                     /translation="MKILLFIFLLPVIFLANIAVADNIYFNFDYALFRGEDNNSIVEI
                     FYSVNQTSLKHEKSETGFEGAAMINISILDVLTNEFVISNTYKTPSLLADTSNKSSAQ
                     KLVGQVNYVLKAGKYKLNITGSDFNDESKQDVFEVEINVEPDKINSISMSDIELATDI
                     KKSNNNQSIFYKNTLEVIPNPSSLYGMNLKEMFYYVEIYGLIIENISDNYKIDYSILN
                     LNNDVLISQFKDAKRNASDRVDYGKINIDSLSSGTYIFRITVSDSTRVVSVSTEKKFY
                     IFNNSGNQVKTGEDDYLRSEFARKAEKELDDEFDKMNYLLTVKQIDIYDSFKNLEEKR
                     KFIYLFWKSKEYATGIPYAKIKLDYFRRVDEADNKFKESYGAGWKTDRGRIYIIYGKP
                     DEIELHPFQASTKSYQIWKYYSVEGGGDCIFIELQPGTDVYWLVSSTFRTELRNDRWE
                     EQLVIN"
     gene            1529..2452
                     /locus_tag="PLX80_13285"
     CDS             1529..2452
                     /locus_tag="PLX80_13285"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HQY21809.1"
                     /translation="MNKNVGIVIVSFLFALVLWLYLNLNQSFSLDLSIPVIINSSKTQ
                     ALAEEIPNNIDVKIRGKGWNLLNILISQDLVYDLDISKMKKDSKIITEQFVNERLKLQ
                     TDITVIDINPDTIDINFDKVSEKKVPVRNNIVVNLRDGYSIIGDAKLNPDSVSIKGAS
                     YLLNKIKYIPTVTKVFNNVNSNISGTITLKDTLQNLLKVEKSSVDFFIKVQLSAEKNY
                     EDIEVKILNVPEDKEVLLIPPKITISIRGGVEELTSISASELIPNIEYNSIEEDTLGF
                     IIPELVLPENTNLMKIEPQKLQYIIKKKQEN"
     gene            2449..3213
                     /locus_tag="PLX80_13290"
     CDS             2449..3213
                     /locus_tag="PLX80_13290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQY21810.1"
                     /translation="MIEYLKTELRSLFSEIKKLDFKVTYIFVSIAIIIFLSIALASPN
                     FYYQNVGKDRLLSRIYWFLTDGSIMFLLPLLSIKFIFKENLKEYGFTWGDKKFGFITS
                     GIFLIAMLITVWIVSGSPDFASAYPQGGSRVSESFGMFIIYELCILVYMLGWEFMWRG
                     YTLFGLKPKFGYYSVFIQMIPFFILHKGKPELELFASIFAGLILGVQALRSGSFLYAW
                     ILHWLVMLSIDSISVLRSGKNIYGIGIDDFFNLILR"
     gene            3218..3937
                     /locus_tag="PLX80_13295"
     CDS             3218..3937
                     /locus_tag="PLX80_13295"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQY21811.1"
                     /translation="MMKLSVNIDHVATLREARGEIEPDPVIAAGICEIAGAEGIVCHL
                     REDRRHINDRDLRLLKETVQTKLDLEMAATDEMLKIAIETLPEIVTLVPEKRSELTTE
                     GGLNLASDMQRYKDFISELKSKDIIVSLFIDPDPVNIDYAIELSADMIEIHTGKYANM
                     RSEKDILMELSKIASISSLASEQGLIVTAGHGLNYKNIIPITNISEISEVSIGHSLIS
                     RAVFTGLHKAVEDMLLLINRN"
     gene            3961..4707
                     /gene="lptB"
                     /locus_tag="PLX80_13300"
     CDS             3961..4707
                     /gene="lptB"
                     /locus_tag="PLX80_13300"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQY21812.1"
                     /translation="MSELKAKSLVKEYKKRRVVDKVSIELKQGEIAGLLGPNGAGKTT
                     TFYMICGMIKPDEGEIEIDKEIVTDFPMYKRANLGIGYLPQEASVFRNMTVRENILSI
                     LQFKKLDKDERESKCESLLKDFNITKIADSKGYVLSGGERRRTEIARCLASEPKFILL
                     DEPFAGIDPIASEEIMKLVANLKTRGIGILITDHNVHETLSIVDRAYILIEGKIFRSG
                     SAETLANDELVRKHYLGENFNLDRYIHKVE"
BASE COUNT         1685 a          687 c          855 g         1498 t
ORIGIN      
        1 agttactgaa aactttgacg ccaatcagta ctttactata ctgttaggtt tctgaaatca
       61 gattattaat tttatttcat taaacaaagg taatcaatat atttgattac ctttttaatt
      121 taaatctttt gaatcattta aatcttagaa tgaaaatact tttatttata tttttacttc
      181 cagtaatttt tcttgcaaac attgcagttg ccgataacat ttattttaac tttgattatg
      241 cattattcag aggtgaagac aataattcca tcgttgaaat attttattca gttaatcaaa
      301 cttcacttaa gcatgaaaaa agtgaaactg gttttgaggg agctgctatg ataaacattt
      361 cgatacttga tgttttaact aatgaatttg taatatcaaa tacttataaa actccatcgc
      421 ttttagctga tacttcaaat aaatcttctg ctcagaaatt agtcggacaa gttaattatg
      481 ttttgaaagc aggaaagtac aaattaaata taacagggag tgactttaat gatgaatcga
      541 aacaggatgt ttttgaagtt gaaattaatg ttgagccgga caagattaat tctatatcaa
      601 tgagtgatat tgagctggct acagacatta aaaaatcaaa taacaatcaa agtatttttt
      661 acaaaaacac ccttgaagta attcctaatc cttcctctct ctatggaatg aatcttaaag
      721 aaatgtttta ctatgtcgaa atttacggac tgattattga aaatatttcg gataattata
      781 aaatagatta ttctatttta aatctaaaca atgatgtcct gatctcccag tttaaagatg
      841 caaaaagaaa tgcttctgac agggttgact acggaaaaat aaacattgac agcctcagca
      901 gtggtaccta tatttttaga ataacagttt ctgattcaac cagagtagta tcagtaagta
      961 cagaaaaaaa gttttatata tttaataatt caggtaatca ggtaaaaaca ggagaagatg
     1021 attacctaag aagtgagttt gcccgtaaag ccgaaaagga attagatgat gaattcgata
     1081 aaatgaatta tcttttaact gttaagcaga ttgatattta tgacagcttt aaaaatcttg
     1141 aagaaaaaag aaagtttatt tatctgttct ggaaatcaaa agaatatgca accggaatac
     1201 cttatgcaaa gattaagctt gattatttta gaagggtaga tgaggctgat aataaattta
     1261 aagaatctta tggtgcagga tggaaaacag acagaggaag aatctatata atctacggaa
     1321 aacctgacga gatcgagctg catccttttc aggcgtccac aaaaagttat cagatatgga
     1381 aatattattc agtagaagga ggaggagatt gtatctttat tgaactgcag cccggtacgg
     1441 atgtttactg gctggttagc agcacattca gaactgaatt gagaaatgac agatgggaag
     1501 aacagcttgt tataaattag attaacatat gaataaaaat gttggaatcg taatagtctc
     1561 ttttttattt gctctggtac tctggctgta tctgaatttg aatcagagtt tttctcttga
     1621 cctgtcaatt ccagtaataa taaattcatc aaaaactcaa gccctggctg aagaaatccc
     1681 aaataatatt gatgtaaaaa ttagagggaa aggctggaac cttttgaata ttcttatttc
     1741 acaagatctt gtttatgatc tggatatttc aaaaatgaaa aaagattcta aaattattac
     1801 ggaacaattt gtaaatgaaa gactaaaact tcaaactgat ataactgtga tcgatattaa
     1861 tcctgacact attgatatta attttgataa agtatcagaa aaaaaagtac ctgtaagaaa
     1921 taacatagta gtaaatctaa gagatggcta tagtataatt ggtgatgcaa aactgaatcc
     1981 cgattctgtt tcaattaaag gagcttcata tcttttgaac aaaataaaat atatacccac
     2041 agttacgaag gtttttaata atgtgaattc aaatataagc ggtacgatta ctctaaaaga
     2101 tacacttcag aatttattga aagttgaaaa atcatcagtg gattttttta taaaagttca
     2161 gctatctgct gagaaaaatt atgaagacat tgaagtaaaa attttaaacg tacctgaaga
     2221 taaagaagta cttttgattc ctccaaagat aacgatttcc atcagaggtg gcgtcgaaga
     2281 attaacaagt atttccgcct ctgaactgat tccaaatatt gaatataata gtattgaaga
     2341 agatactctt ggttttataa ttccagaact cgtgcttccc gaaaatacaa atcttatgaa
     2401 aatagaacct caaaaacttc aatacataat aaagaaaaaa caagaaaatt gattgagtat
     2461 ttaaagacag aactccgcag tttattttca gaaattaaaa aactggattt caaagtaaca
     2521 tatatctttg tttcaattgc gataattatt tttctgtcaa ttgcactggc ttctccaaat
     2581 ttttattatc agaatgtcgg aaaggacaga ctgctttcaa ggatctattg gtttctgact
     2641 gacggatcga tcatgttcct gttgccgctg ctttcaataa aatttatatt caaagaaaac
     2701 ctgaaggagt acggctttac ttggggtgat aaaaagtttg gatttataac ttccggaatt
     2761 tttctgattg ctatgctgat aactgtatgg atcgtttcgg gttcacctga ttttgcttca
     2821 gcttacccgc agggaggatc aagagtcagc gaaagttttg gaatgtttat tatttatgaa
     2881 ttatgcattc ttgtatatat gctcggatgg gaatttatgt ggagaggtta tactctattt
     2941 ggattaaaac caaagttcgg atattactct gtctttatac agatgatccc tttttttatt
     3001 ttgcataaag gaaaacctga attagaatta tttgcatcca tttttgccgg acttatactt
     3061 ggtgtgcagg cgcttcgaag cggatcattt ctgtatgcct ggatactgca ttggctggtg
     3121 atgctttcaa ttgatagtat ttcagtttta agatccggaa aaaatatcta cggaatcggt
     3181 attgacgatt tttttaattt aattttaaga taaataaatt atgaaacttt cagtaaatat
     3241 agatcacgtc gctacattac gtgaagcacg aggtgagatt gaacctgatc cggtcattgc
     3301 agcaggcatt tgtgaaattg caggtgctga aggaattgtt tgccacctcc gggaagacag
     3361 aaggcatatc aatgacagag atcttagact tctaaaggaa actgtacaga ccaaactcga
     3421 tctggaaatg gcagcaacag atgaaatgct taagattgca attgaaacac ttccggaaat
     3481 tgttactctc gttcctgaaa aaagaagtga actcactaca gaaggaggtt tgaatcttgc
     3541 atcagatatg cagaggtata aagattttat ttctgaactc aaatccaaag acattattgt
     3601 aagcctcttt attgatcccg atcctgtgaa cattgattat gcaatcgaat tatcagccga
     3661 tatgatcgag attcatacag gaaaatatgc aaacatgaga tctgaaaaag atattctcat
     3721 ggaactcagt aagatagcaa gtatctcatc acttgcatcg gagcagggac ttatcgttac
     3781 tgccggtcat ggattgaatt ataaaaacat tattccgatc acaaatattt ctgaaataag
     3841 tgaagtaagt ataggacatt ctctgatctc aagagctgta tttacaggac ttcataaagc
     3901 agtagaagat atgctgctgc tcataaacag aaattaatca taaccaaatt cacaagtaca
     3961 ttgagcgaat taaaagccaa aagtttagta aaagaatata aaaaaagaag ggttgttgat
     4021 aaagtttcca ttgagcttaa gcaaggcgag atagccggtt tgcttggtcc aaacggtgca
     4081 ggaaaaacaa ctacatttta tatgatctgc gggatgatca agcctgatga aggtgagatt
     4141 gaaattgata aagaaatcgt aacggatttt ccaatgtata aaagggctaa tcttggaatt
     4201 ggttatcttc ctcaggaagc atcggttttt agaaatatga cagtcagaga aaatattttg
     4261 tcaatacttc agtttaaaaa gcttgataaa gatgaaaggg aatctaaatg tgaaagtctt
     4321 ctaaaagatt tcaatattac aaaaattgct gactcaaaag gatatgtact ctctggcggt
     4381 gaaagaagga gaactgaaat tgcaaggtgt cttgcgtctg agccaaaatt tattttactg
     4441 gatgaaccct ttgcaggtat agatccgatc gcgtcagagg aaattatgaa actcgttgca
     4501 aatcttaaga caagaggtat cggaattctc atcactgatc ataatgtcca tgaaacactt
     4561 tccatagttg acagagctta tattttaatt gaagggaaaa tattcagatc gggatctgca
     4621 gaaactcttg ctaatgatga actcgttaga aaacattacc tgggtgaaaa ttttaatctc
     4681 gacagatata tccataaagt agagtaaaac tcaatttcat ttttt
//
LOCUS       DAORVQ010000236         4593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_13749_length_4593_cov_6.079550, whole genome shotgun sequence.
ACCESSION   DAORVQ010000236 DAORVQ010000000
VERSION     DAORVQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13749_length_4593_cov_6.079550"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLX80_13305"
     CDS             complement(<1..185)
                     /locus_tag="PLX80_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQY21813.1"
                     /translation="MSNEILILFFIVGIVMVGSGMIISHIIAPSSKNSVKEETYECGI
                     ETTGTSKIQFKVGYYLFA"
     gene            686..2374
                     /locus_tag="PLX80_13310"
     CDS             686..2374
                     /locus_tag="PLX80_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21814.1"
                     /translation="MSKFTKVIFALMFAVMLFPYEYAMSQFQTGDVFVAVGNGQVQWR
                     QPNGTLVQTLNTGQGGFTTGMAIDSASKLYVTNFSVSTVSVFDKFGVLQGNFGSGYQT
                     PESIVFDNQYNVYVGNLSNGIRKFDYNGNFIATVGPNTDFHDLNSDQCTGYITHEGSI
                     IDRHNYCTNTSMGQFATGLGGSAFALRIRQNGEVLVGNGVNILRLNAAGAIIQTYDVA
                     GENAWFALNLDPDGMSFWSADFSTANVYRIDIATGNVITTFNTGTGGNTVFGLTVNGE
                     ITAATVLNIEVAPTNSTKYINNQHCVTATVTDQNAVPQENVNVVFVVGGVNAPQNFAG
                     NTNALGQIEFCYMGTNIGRDTIFATIGTSGRSDTAYVLWEDPLPVELSSFTSSVSSND
                     VKLNWSTSEEINNSGFDIERSSNGTWTKVGNVNGNGSTNEIKNYSYTDKNVSSGTYNY
                     RLKQIDYNGSFEYYNLSNEVIVGVPDKFSISQNYPNPFNPSTKIDFQLPTDGNVKLSV
                     FDNSGKQVEVLSDGFKSAGYYSINFNATNLSSGIYFYKIEFNGISKVMKMSLLK"
     gene            2532..>4593
                     /locus_tag="PLX80_13315"
     CDS             2532..>4593
                     /locus_tag="PLX80_13315"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21815.1"
                     /translation="MFAFVNNNSSKAALDSKGTDFWLMFNGNVSAPTITLFITSNINT
                     SGNVSIPGLAFSVPFNVTANVVTSVVLPSSVATHTSNVVDNKGIHVTSLDEVTVYGLN
                     YAPFTTDAYLGLPTDILGTDYTVLSYKNTSGNGVQFGVVGTVNATTVTITPSVTTGPR
                     IAGIPYNIILNQGDTYELTNSSGTSDLSGTTITSNQPIGVMGSNRCANIPGGFAFCDH
                     ICEMIPPNSTFGQNFVTVPLASRTNGDTWRILASQNSTIVNINGVAQPAINAGQFIET
                     VLTGQSTINSDKPILVAQYSNGSSFSGNPGDPFMMIIPSYEQYLANYTLSTVTGYVAH
                     YINVVAPNAIVGSLTLDGAPVPVIEFTPIGVTGFSGAQIAVTPGSHTLDGSLPFGAFQ
                     YGFNTDDSYGYPGGLNLAPIATVSSLDVTPETSTRFINTQHCVDGLVLDQNNAPVVGV
                     RVDYLVSGVNPGAGFAFTNGSGIAQYCYTGINIGKDTIIGSTGNLVDTVLVTWDDPLP
                     VELNSFVSMVSEKNVTLSWTTSSEINNSGFDIERSSLNLEWAKVGSVNGNGTTLETKN
                     YIFTDKNLNSGVYNYRLKQIDYNGNFEYFNLENEVIIGLPNNFTLLQNYPNPFNPSTK
                     IEFNIPADGNVKLSVYDINGKLVSVLTDGNKTAGYYTVDFNGSGLSSGIYFYKIEFNG
                     ISKVM"
BASE COUNT         1595 a          712 c          813 g         1473 t
ORIGIN      
        1 gcaaatagat aataaccgac tttgaattga attttagaag tgcccgtagt ttcaataccg
       61 cattcataag tttcttcttt aactgaattt ttagaggacg gcgcaatgat atgagaaatt
      121 atcattccgc tgcccaccat tactattccg acaataaaaa ataagatcag tatttcatta
      181 ctcatgattt aagtacattt tatttccgat tgcgttttaa aagtttgatt tgtcaaaata
      241 tgaatatggt gtagaattgt aaagttaaat atttattaat tcttaaaaat ttaacacata
      301 tagttttctc aataaaatta ctaagtaggt tcactcattg aattttaaaa agaaaatctc
      361 taagtttgta ttaaattaag aaacaatatt tgatccccat tcttaacttt tgataatttc
      421 aaaatattat agaagtattc ttatttaagt tagaattttt ataaaattaa aaaacttttg
      481 ttaaattaaa tttaattaca gattttttat aaattatttt aattttgtaa attatttagt
      541 tttaaaatat ctaaattaaa tatatttttt taataaatgt tgattttaaa aaaattcaaa
      601 aagagtttta aatttccaac tcaaaagata cagtaacata tccaaaaata aacactaact
      661 attaatttaa ccgaaaggaa aaaaaatgag taaatttaca aaagtaatat ttgctttaat
      721 gtttgcagta atgctttttc cgtatgaata cgcaatgtcc cagtttcaga ctggtgatgt
      781 attcgtagct gtaggaaacg gtcaggtgca atggagacaa cctaatggaa ccttagttca
      841 gacattgaat acaggtcagg gtggctttac aacgggaatg gcaattgatt cagccagtaa
      901 gctttatgtt acaaattttt ctgtgagtac agtatcagta tttgataaat ttggagtact
      961 tcaggggaat ttcggatcag gatatcaaac accagaatca atcgtttttg acaatcaata
     1021 caatgtttat gtaggtaatc taagtaatgg tatcaggaaa tttgattata atggaaattt
     1081 catagctaca gtcggtccaa atacggattt tcatgatctg aactcagacc aatgtacagg
     1141 ttatattact catgaaggaa gtataattga cagacataac tattgtacaa acacttccat
     1201 gggtcagttt gcaacaggac ttggaggaag tgcatttgct ttaaggatca gacaaaatgg
     1261 agaagttttg gttggaaatg gtgtaaacat tttaagatta aatgctgcag gagcaatcat
     1321 acaaacttat gatgtagcag gtgagaatgc gtggtttgct ttgaatctgg atccggacgg
     1381 aatgtcattc tggagtgctg attttagtac tgcaaatgtt tacagaattg atattgctac
     1441 cggaaatgtg attactactt ttaataccgg aaccggtgga aatacagtat tcggacttac
     1501 tgtaaatggc gagatcacag cagctacagt actgaatatt gaagtagcac caacaaattc
     1561 aaccaagtat atcaataatc agcattgtgt gacagctacg gttacagatc agaatgcagt
     1621 acctcaggaa aatgtaaatg tggtttttgt agtcggtgga gtaaatgcac cacagaactt
     1681 tgcaggtaat acaaatgcac ttggtcagat tgaattctgt tatatgggaa caaacatagg
     1741 aagagataca atctttgcaa caattggaac atcaggaaga tcagatacag cttatgtact
     1801 ctgggaagat ccacttccag ttgagctgtc ctcatttact tcttcagttt cttcaaacga
     1861 tgtaaaactg aactggtcaa cttctgaaga gatcaataat tcaggatttg atattgagag
     1921 atcatcaaac ggaacatgga caaaggttgg aaatgtaaac ggtaatggct caacaaatga
     1981 aataaagaat tattcatata cagataaaaa tgtaagttct ggaacatata attacagatt
     2041 aaaacaaatt gattataatg gctcttttga atattataat ctgagcaatg aagttatagt
     2101 tggtgttccg gataaattct caatttcaca aaattatccg aatcctttca atccatcaac
     2161 aaagatcgac ttccagttac caactgatgg aaatgtaaaa ttatcagtat ttgataatag
     2221 cggaaaacag gttgaagtat tgtcagacgg attcaaatca gctggatact attcaattaa
     2281 tttcaatgca acaaatctgt caagcggaat ttatttctat aagatagaat tcaacggcat
     2341 cagcaaagta atgaagatgt ctttattgaa ataaaatttt tttaaaataa taacataaag
     2401 ccggataata tgtccggctt tatttgattt aaagatctaa aaatgatttt agaaattaat
     2461 taattaatat aactgaaagg aaatatatga aatcgaaaag ttacattctc aagttaatca
     2521 tattaatcgt gatgtttgct tttgttaata acaattcttc aaaagcagct ttagacagta
     2581 agggtacaga tttctggctg atgtttaacg gaaatgtctc agctccaaca attaccttat
     2641 tcattacttc aaatataaat acttcaggaa atgtcagtat tccaggactt gctttttcgg
     2701 tgccatttaa tgttacagct aatgtagtta cttctgtagt tcttcccagc agtgtagcaa
     2761 cgcatacaag taatgttgta gataataaag gaatacatgt cacatcactg gatgaagtaa
     2821 cggtctatgg attgaattat gcaccattta caacagacgc atatcttgga ctaccgactg
     2881 atattctcgg gacggattac actgttctgt catataagaa tacgagtggt aatggtgtac
     2941 aatttggagt tgtcggaaca gttaacgcga caacagttac aattacacct tcagtaacaa
     3001 caggtccgag aattgcaggt ataccatata atatcattct taatcaggga gatacatatg
     3061 aattaacaaa ctcatctggt actagcgatc tttctggtac aacaatcaca tcaaatcaac
     3121 cgattggagt tatgggttct aacagatgtg ctaatatacc cggaggtttt gcattctgtg
     3181 atcacatttg tgaaatgatc cctccgaatt caacctttgg acaaaatttt gttacagttc
     3241 cactggcttc gagaacaaac ggtgatacat ggagaatact tgcttctcaa aattccacaa
     3301 tagtaaatat taacggagtt gcccagccgg caattaatgc cggacaattt attgaaactg
     3361 tattgacagg acaatcaaca attaattcgg ataaacctat acttgttgct caatattcaa
     3421 acggatcttc attctcagga aatcctggtg acccgttcat gatgataatt ccttcatacg
     3481 aacaatatct tgcaaactat acactttcaa cagtaactgg ttatgtggct cattatatta
     3541 acgtagttgc tcctaatgca atagtcggat cacttacact tgatggtgca cctgtacctg
     3601 ttatagaatt tacacctatc ggtgtgacag gattttccgg tgctcaaatt gctgtaactc
     3661 ccggctcaca tacacttgat ggtagtttac cttttggagc atttcaatac ggttttaaca
     3721 ctgatgactc ttacggttat ccaggcggac ttaatttagc tcctattgca acagtatcga
     3781 gtctggatgt aactccagaa acttcaacac gatttataaa tacacaacat tgtgttgacg
     3841 gacttgttct ggatcaaaac aatgcccctg tagtcggtgt aagggttgat tatctggtta
     3901 gtggagttaa ccccggagca ggatttgcat ttacaaatgg aagcggaata gctcagtatt
     3961 gttatacagg aattaatata ggaaaggata ccataatcgg atctaccggt aatcttgttg
     4021 atacagtttt agttacatgg gatgatccat tacctgttga gttaaattcg tttgtgtcaa
     4081 tggtttcaga aaagaatgta actctttcat ggactacttc atctgaaata aataattccg
     4141 gatttgatat tgagcgttca tcattaaatt tagaatgggc aaaagttggt tcagtaaatg
     4201 gaaacggaac aacattagaa acaaaaaatt atatttttac agacaagaat ttaaacagcg
     4261 gagtttataa ttacagattg aaacaaattg attataatgg aaactttgaa tatttcaatt
     4321 tagaaaatga agtgattata ggtttgccaa ataatttcac attattacaa aattatccaa
     4381 atccattcaa tccttcaacc aagattgaat tcaatattcc ggctgacgga aatgtaaaac
     4441 tttcagttta tgatataaac ggtaagttgg tttcagttct tacagacgga aataaaactg
     4501 ctggatatta cacagtagat ttcaatggtt ctggtctttc aagcggaatt tatttctata
     4561 agatagaatt caacggcatc agcaaagtaa tga
//
LOCUS       DAORVQ010000237         4474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_14378_length_4474_cov_6.431998, whole genome shotgun sequence.
ACCESSION   DAORVQ010000237 DAORVQ010000000
VERSION     DAORVQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14378_length_4474_cov_6.431998"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..438
                     /locus_tag="PLX80_13320"
     tRNA            365..438
                     /locus_tag="PLX80_13320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            564..1586
                     /locus_tag="PLX80_13325"
     CDS             564..1586
                     /locus_tag="PLX80_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQY21816.1"
                     /translation="MKNKLLFLASLSFVFIISLFTDNIDPYYYTILIYIGINIILASS
                     LNLINGYTGQFSLGHAGFMAIGAYLSASISTYFAPFFINNFGTGVFGDTISFVIVLII
                     GGIAAAFAGVIVGVPSLRLKGDYLAITTLGFAEIIRVIIQSLDVVGASQGFRGAYIFE
                     NGVKAAIIPADMSSVPYEFFTIPKYTDFFWAFGFVVVIIFFIKNLMSSSYGRGFLAVK
                     DDEIAAESMGVNTTKFKVTAFVSGAFFAGIAGALYAHFNLYLNPEDFNFLKSVEIVVM
                     VILGGMGSMIGVIIAAVLLTILPEFLRGIAEYRMIIYALLLIVMMILRPQGLFGVQIR
                     SRKKKK"
     gene            1593..2360
                     /locus_tag="PLX80_13330"
     CDS             1593..2360
                     /locus_tag="PLX80_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21817.1"
                     /translation="MSLLEIKDCTMRFGGLTCVDKLNTHVGENELIGMIGPNGAGKTT
                     VFNIITGVYDPTEGDVLFKGKSVVPLKPFEVAEVGISRTFQNIRLFKSLSVRDNIRVS
                     FVKSIKSGFFLSTFQLGKYETEESEIENKIDELLEIFGLSDEADEYASSLPYGDQRKV
                     EIVRALATSPQLLLLDEPAAGMNPTEKTELMSLIKMVKNEFKISILLIEHDMKVVMGV
                     CERIFVLDYGKKIAEGNPEVIKKDPKVIEAYLGETHH"
     gene            2372..3079
                     /locus_tag="PLX80_13335"
     CDS             2372..3079
                     /locus_tag="PLX80_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21818.1"
                     /translation="MLEIKDLHVSYGAIKALKGINLVVKDGSIVTLIGANGAGKTTLL
                     RTISSLLKPVSGVIEFEGKDITNLAPDKIVELGISQSPEGRMIFSNLTVKENLEMGAY
                     IRKDKHGLNEDLEIIYELFPRIKERLKQLGGTLSGGEQQMLAISRALMSKPKLLLLDE
                     PSLGIAPILVRTIFEQIVELNKKTGITILLVEQNANMALSISDYGYVMETGEIILEGV
                     AHSLAQNEEVRKAYLGE"
     gene            3279..4436
                     /locus_tag="PLX80_13340"
     CDS             3279..4436
                     /locus_tag="PLX80_13340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQY21819.1"
                     /translation="MKLADRMSNLGTETAFEVLAKARKLEAEGKSIIHLEIGEPDFPT
                     PENICNAAIESIKAGDTHYTPSAGIPEVRKSIADYISRTRGIDVHPDEVVMTPGAKPI
                     MFYAILALINPGDEVIYPNPGFPIYESMIKFVDGVPVPIQLKEEKNFAFDPEDLRKLI
                     TPKTKMLIMNTPQNPTGGILTEDVIRKIAEIVKDREDITILSDEIYSRLIFQGDHFSI
                     TQIPGFKDRTIILDGFSKTYAMTGWRAGYGVMNKDLAAVTAKLMTNSNSCTNAMVQKA
                     CIEAYEGSQDSVEEMKKEFMIRRDIIVEGLNSIDGFSCNLPLGAFYAFPNITKTGMGS
                     RELADHLLYKGGVAALSGTAFGVFGEGYLRFSYANSRENIKEAVKRIKDVM"
BASE COUNT         1478 a          713 c          884 g         1399 t
ORIGIN      
        1 attgacatgt acattgtact tttcagcggc ctggttgtag gaatgttatt ctcaggaagc
       61 tctctgataa tcatgatttt ttgttctgaa gtatagcgtt ttctttctga catgatggaa
      121 tttactttcg tattttttta caaatataat aattccgaaa ttccatttcc acttgaagca
      181 ttacaccttc atatctttat gtctaatttt tattttttat tcttgaagta tttaataact
      241 gagtttgaat aatttcaatg tatcgattgt aaattaaaca tttataattg aaaattaaaa
      301 agtggtttga aattcagaaa ttaaaaacat atttttgtaa cttattgatt caaaaaaaga
      361 ttaagggcga ttagctcaat tggctagagc acctcgctta caccgagggg gttataggtt
      421 cgagtccttt atcgcccact ttaatttcag aaaataagta ctccgctgca ttcaggatta
      481 cttattcttt tttaaacctc tcactaaatt aaaaaacatc tgaaaattct cattgaaaga
      541 tccgtattca aatttaattt ttaatgaaga ataaattatt atttcttgca tctctttcct
      601 ttgtttttat aataagtctc tttactgata acatcgatcc atattattat accattctta
      661 tttatattgg tattaatatt attcttgctt ccagtcttaa tttaatcaac ggttatacag
      721 gtcagttctc actcggtcat gctggtttca tggcaatcgg agcttattta tctgcatcga
      781 tcagtacata ctttgcgcca ttttttataa ataattttgg cacaggtgtt ttcggcgata
      841 cgatctcttt tgtaatagtg ctgatcatag gtggtattgc agcagctttt gcaggagtca
      901 ttgtaggcgt tccgtcattg agacttaaag gcgattatct tgcaattact acattgggat
      961 ttgccgagat catcagagta ataattcaga gtcttgatgt ggtaggagca tcgcagggat
     1021 ttcgcggagc atacattttt gagaatggag tgaaggcagc gataatccct gctgatatgt
     1081 cttcagttcc atacgaattt tttaccatcc caaaatacac agattttttc tgggcatttg
     1141 gttttgtagt tgtgattatt ttctttataa aaaatctaat gagctcatca tacggcagag
     1201 gattccttgc tgtgaaagat gatgagatag ctgcagaatc aatgggagtc aatacaacta
     1261 aattcaaagt cactgcattt gtcagcggag cattctttgc cggaattgcc ggcgcattat
     1321 atgcacattt taatctgtat ctgaatcctg aagattttaa ttttttgaag tcagttgaga
     1381 ttgtagtaat ggtgatactc ggtggaatgg gaagtatgat cggcgtaata attgctgcag
     1441 tacttctgac gatactgccc gaatttctca gaggcattgc tgaatacaga atgataatct
     1501 atgctttact tttaattgtt atgatgatac tgagaccgca gggattattc ggagttcaga
     1561 taagatcaag aaaaaaaaag aaataaaata aaatgagctt actcgaaata aaagactgta
     1621 ctatgagatt cggcggattg acatgtgtcg ataagctgaa tactcacgtc ggcgaaaatg
     1681 aactgatcgg aatgatcggt ccgaacggcg cagggaaaac tacagttttt aatattatca
     1741 ccggtgtata tgatccgaca gaaggggatg tgcttttcaa aggtaaaagt gtcgtacctt
     1801 taaaaccatt tgaagttgct gaagtaggta tcagcaggac atttcagaac atcagacttt
     1861 ttaagagcct gagcgtccgc gataatatca gagtatcatt tgtaaaaagc attaaatcag
     1921 gatttttcct ttcgacattt caacttggaa aatacgagac tgaggaaagc gaaatcgaaa
     1981 ataaaattga tgaacttctt gagattttcg gattatcaga tgaggcagat gaatatgcga
     2041 gttccctgcc ctatggtgat cagaggaaag ttgagattgt cagagcactt gcgacttcgc
     2101 ctcagttact tttgcttgac gaacctgcgg ctggaatgaa tccgactgaa aaaactgaac
     2161 tcatgagtct gatcaaaatg gttaaaaatg aatttaagat ttcaatactt ctgatcgaac
     2221 atgatatgaa agttgtgatg ggtgtatgtg aaagaatatt tgtacttgat tacggaaaaa
     2281 agatcgcaga gggaaatccc gaagtcatta aaaaagatcc gaaagtgatc gaagcttatc
     2341 ttggggagac gcatcattga ttagtcagaa tattcttgag ataaaagatc tgcatgtaag
     2401 ttacggtgct attaaagctt tgaaaggaat taatttagtt gtcaaagatg gatccatcgt
     2461 tacacttatc ggtgcaaacg gtgccggtaa aactacactt ctcagaacaa tttcttcctt
     2521 gctcaaacct gtttcaggag tcatagaatt tgaaggtaaa gatattacaa atttagctcc
     2581 ggataaaatt gttgaacttg gaatatcaca gtctcctgaa ggaagaatga tattctcaaa
     2641 tcttacagtt aaagagaatc tggaaatggg tgcttatata agaaaagaca agcacggatt
     2701 aaatgaagat cttgaaatta tttacgaact gttcccaaga attaaggaaa gactaaaaca
     2761 attaggcggt actctgagcg gaggtgaaca gcagatgctc gcaatttcca gagcacttat
     2821 gtccaagccg aaattactat tacttgatga gccatcactt ggtatagcac ctattttagt
     2881 cagaactatt tttgaacaaa tagtagaact aaataaaaaa acaggaatca ctattttact
     2941 tgtagaacaa aatgcaaata tggcgctgtc aatttctgat tatggatatg taatggagac
     3001 cggtgaaatt atactcgaag gtgttgcgca ttctctcgct cagaatgaag aagttcgcaa
     3061 agcatactta ggtgaatgat ctgagatcaa ttttaaattt taaatattaa ataaaaaatt
     3121 atgcaattta taaaataagt tgaataatta aacttaatgt tttcaatttg aatatgaatc
     3181 aatgtgtcta tgttttctat gtgtttctat gtgtttctaa agagaaaaaa tctgatagca
     3241 ataaacagaa aaaaatataa aaataaaaat ataacatcat gaaattagca gacagaatgt
     3301 caaatctcgg aacagagaca gcttttgaag tacttgcaaa agcgagaaag cttgaagccg
     3361 aaggtaaaag tattattcat cttgagatcg gcgagccgga ttttccaaca cctgaaaata
     3421 tttgcaatgc agcgattgaa tctattaaag cgggtgatac acattatact ccttcagcag
     3481 gtattcccga agtcagaaaa tccattgcag attatatcag caggacaaga ggaattgatg
     3541 tccatccgga cgaagtcgtc atgactcctg gtgcaaagcc gatcatgttt tatgcaatac
     3601 ttgcgctcat taatccgggt gatgaagtga tctatccaaa tccgggtttt cctatttatg
     3661 aatcgatgat aaaatttgtc gatggcgtcc ctgttcctat tcagctcaaa gaagaaaaaa
     3721 atttcgcatt cgatcctgaa gatctaagaa aactgataac tccaaagacg aaaatgctta
     3781 tcatgaatac tccgcagaat cctaccggcg gtattcttac agaagatgtg atcagaaaaa
     3841 ttgccgagat agtaaaagac agagaagaca ttactattct tagtgatgaa atttacagca
     3901 gacttatatt tcagggagat catttcagca ttacacagat ccccggcttt aaggacagga
     3961 cgattattct cgatggcttt tccaagactt atgcaatgac aggatggaga gccggttatg
     4021 gagttatgaa taaagacctt gctgcagtga ctgcaaaact tatgacaaac agcaattcct
     4081 gtacaaatgc aatggtacag aaagcatgta tagaagcata tgaaggttca caggattcag
     4141 tagaggaaat gaaaaaagaa tttatgatca gaagagatat cattgtggaa ggtctgaatt
     4201 caattgatgg attctcgtgt aatcttccgc ttggcgcttt ctatgcattt ccgaatatta
     4261 caaagacagg aatgggatcc agagaactcg cagatcatct gctttataaa ggaggtgttg
     4321 ctgcattatc aggaactgct ttcggtgtat ttggtgaagg ctatctcaga ttctcatatg
     4381 caaactcaag agagaatata aaagaagctg ttaaaagaat taaagatgtg atgtgatttt
     4441 ttttaaaaca tcatttatat ttaaaagcct gtaa
//
LOCUS       DAORVQ010000238         4462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_14464_length_4462_cov_6.741774, whole genome shotgun sequence.
ACCESSION   DAORVQ010000238 DAORVQ010000000
VERSION     DAORVQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14464_length_4462_cov_6.741774"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLX80_13345"
     CDS             complement(<1..235)
                     /locus_tag="PLX80_13345"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HQY21820.1"
                     /translation="MEGKLNTGQEKKDSDLIMDISAKEKLELKTQELMPTILIGLGGT
                     GKETLLRIRRQFVEKYGSINDFPIISYLYIDTDN"
     gene            complement(266..1060)
                     /locus_tag="PLX80_13350"
     CDS             complement(266..1060)
                     /locus_tag="PLX80_13350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY21821.1"
                     /translation="MKKLYALILGSSSGFGKAIAVGLAKSGVNIIGVHLDRSTTMPDV
                     ENLKEELKSYNVDVHFYNVNAADSGKRTDVINNIKEIFSKQEDPELKVLLHSLAFGTL
                     KHFIAKESSEMINQKQIEMTLDVMANSLVYWTQDVIREGLMHRGGKIYAMTSSGGQRQ
                     IEFYGAVSAAKACLESYIRQFAMELGSMGISANAIRAGVTATPALSKIPKSENIVANA
                     IQRNPEHRLTKVEEVAEFIVDNYKNDSHWMTGNIINLDGGESVVEL"
     gene            complement(1099..1656)
                     /locus_tag="PLX80_13355"
     CDS             complement(1099..1656)
                     /locus_tag="PLX80_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21822.1"
                     /translation="MKTFFKLIIFVSLFLINTGTYAAPKHTAGVQQNVDTTVNSDSLK
                     NNTSTQGTQNTLPIDTSSAEKIETYIQDDTVTKIDSVVTETEDENTLTNTKLFIYILL
                     SVLGLGLFFFIFVINLFKTFHKKKSTRQSLLLSWNLFFIVSIIWLFIVWGIVAAFWTY
                     PSFMIVMIFLFIISLIMTIIAVKSK"
     gene            complement(1666..3003)
                     /gene="accC"
                     /locus_tag="PLX80_13360"
     CDS             complement(1666..3003)
                     /gene="accC"
                     /locus_tag="PLX80_13360"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQY21823.1"
                     /translation="MIKKLLIANRGEIALRIIRTCKEMDIKTVAVYSDADKNSLHVKF
                     ADEAVCIGSPLSKESYLNIPRIMSAASITNADAIHPGYGFLAENAGFAEICLDSKINF
                     IGPLPDMITSMGNKSLAKDTMRAAGVPVVPGSDGVVKDLEEATKLIKKIGVPVMIKAS
                     AGGGGKGMRIVRNISEIESAYNTAKSEAMSSFGNDDVYLEKYIDKPRHIEIQVLGDKF
                     GNVISLGERDCSIQRRHQKLIEESPSPFIDNGLRARMSAAAVLGAKSVNYIGAGTIEF
                     LVDKDKNFYFMEMNTRIQVEHPVTEAITGIDLIREQIRIADNKKLSKKKINMTGHSIE
                     CRINAEDPANNFRPSPGTITAFNQPGGIGVRVDTHCYNGYAIPPYYDSMIGKLIVHAN
                     DRNEAIKKMSRALDEFIIEGIHTTIPFHQMMMHNDSFINNDFDTGFIDNLKKD"
     gene            complement(3064..3558)
                     /gene="accB"
                     /locus_tag="PLX80_13365"
     CDS             complement(3064..3558)
                     /gene="accB"
                     /locus_tag="PLX80_13365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQY21824.1"
                     /translation="MKLDLNYLKKLIKILDSSNISEFEVQEEGSRLRLSKPKNKIAMA
                     SQPFDISSMNFMPAMQQQESESKSDQNIQEMPQTETLKNSKLVEVNSPMVGTFYRSPS
                     PDADSYVSVGKSVSEGSVLCIIEAMKLMNEIESEVSGKIIKILVENAQPVEYNQPLFL
                     IEIE"
     gene            complement(3580..4143)
                     /gene="efp"
                     /locus_tag="PLX80_13370"
     CDS             complement(3580..4143)
                     /gene="efp"
                     /locus_tag="PLX80_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQY21825.1"
                     /translation="MASTSDLKNGVIINFNNELHSVISVEHRTPGNLRAFYQVKLKNL
                     KNGKTMENRFRSGESIQLERLDTGNYQYLYKDGEDYVFMENETFEQVTIAGDIIGKGG
                     NYLKEGEIVQIMFHNSNPLSIEIPPHVFLKVISAPPGVKGDTATGATKPVELETGATV
                     NAPLFINEGDVLKVDSRTGDYIERAKL"
BASE COUNT         1323 a          846 c          660 g         1633 t
ORIGIN      
        1 cattgtcagt atctatataa agatatgata taatagggaa gtcatttata gatccgtact
       61 tttctacgaa ttgtcttcta atcctgagaa gagtttcttt accggtaccg ccaagtccaa
      121 tgagaattgt cggcattagt tcctgagtct tcagctcaag tttttccttt gcagaaatat
      181 ccattataag atcagagtct ttcttttcct gacctgtatt taatttacct tccatctgtt
      241 atttataaat ctcttttaaa attaattata actctactac gctttcacca ccgtccagat
      301 ttattatgtt tcctgtcatc cagtgtgaat catttttgta attatctact ataaactctg
      361 ctacttcttc tactttagta agcctgtgtt caggattcct ttgaattgca ttagctacaa
      421 tattctcact ctttggaatt ttcgacaaag ccggagtagc tgtaactcct gctcttattg
      481 catttgcaga aatacccatg ctgccaagtt ccattgcaaa ttgtcttata taagactcga
      541 gacatgcttt agccgcagaa actgcaccat aaaattcgat ctgtctctgt ccgcctgatg
      601 aagtcatagc gtagatcttt ccgcctctgt gcatcagacc ttcacggatc acgtcctgag
      661 tccagtaaac aagactgttt gccattacat ccagtgtcat ttctatttgc ttctgattta
      721 tcatttcaga agattctttt gctatgaaat gttttaatgt tccaaaagcc agtgaatgta
      781 ataaaacttt tagttcaggg tcttcctgtt ttgaaaatat ttctttaata ttattaataa
      841 catctgttct cttacctgaa tcagcagcat ttacattata aaaatgaaca tcaacattat
      901 aagactttaa ctcttccttt aaattttcta catcgggcat cgtagtcgat ctgtctaaat
      961 ggacaccgat aatatttact cctgattttg caagtccgac agcaatggct ttgccaaacc
     1021 cgctcgatga tccaagtatt aaagcatata gttttttcat aataatattt tacggaaaat
     1081 atacaaaagt ttagaaaatt atttggattt gactgctatt atagtcatta tcaaacttat
     1141 tataaataga aaaatcatca caatcataaa cgatggatag gtccagaatg ctgctactat
     1201 tccccataca atgaataacc agataattga gacaataaag aataaattcc agctcagcaa
     1261 caaagactga cgtgttgatt tttttttgtg aaatgttttg aaaagattta ttacaaatat
     1321 aaaaaagaat agaccaagtc ctaaaactga caacaagata tatataaaaa gttttgtgtt
     1381 tgtcagagta ttttcatcct ctgtttcagt aactacagaa tcaattttag taactgtatc
     1441 atcctgaatg taagtctcaa ttttttccgc agaagaagta tcaatcggaa gtgtattctg
     1501 tgttccctgt gtactggtat tattctttaa tgaatcagaa tttactgtag tgtcaacatt
     1561 ctgctgaacg cctgcagtat gtttcggagc agcatatgtc cctgtattta taagaaataa
     1621 agaaacaaaa attatgagtt tgaaaaatgt tttcaattat attgattaat ctttttttaa
     1681 attatcaatg aatcccgtat caaagtcatt gttgatgaaa ctatcattat gcatcatcat
     1741 ctgatgaaat ggaattgtcg tatggatacc ttcaataata aattcatcaa gagcacggga
     1801 catttttttg atcgcttcat ttctgtcgtt agcatgtaca ataagtttcc cgatcattga
     1861 atcatagtac ggaggaattg catatccgtt atagcagtgt gtatctactc ttaccccgat
     1921 accacccggc tgattaaatg cagttattgt acccggtgac ggtctgaaat tattcgcagg
     1981 atcttcggca ttgattcggc attcaataga atgtcctgtc atgttgatct ttttcttact
     2041 taatttttta ttatcagcaa tccttatctg ctctcttatt aagtcaatac ctgttatagc
     2101 ttcagtcaca ggatgttcaa cctggattct tgtattcatt tccatgaaat aaaaattctt
     2161 atctttatcc actagaaatt ctattgtacc tgcaccaata taattaacag acttcgcacc
     2221 aagtactgct gctgctgaca ttcttgctct taacccgtta tcaataaatg gggaaggaga
     2281 ttcctctatc agcttctgat gtcttctctg aatggaacag tctctttctc caagtgaaat
     2341 tacattacca aatttatctc caagtacctg tatctcaatg tgtcttggct tatctatata
     2401 tttttctaaa tacacatcgt catttccaaa cgaactcata gcttcagatt ttgcagtatt
     2461 gtatgcactt tcgatctcgc ttatatttct cacaattctc attcccttac cgccgcctcc
     2521 tgctgaagct ttgatcatta caggtacacc aatctttttt ataagcttgg ttgcttcctc
     2581 aagatcttta actactccgt cactgccggg aactacaggt actcccgctg ctctcattgt
     2641 atcttttgcg agagatttgt ttcccattga tgtgatcata tccggaaggg gaccaatgaa
     2701 attaattttc gaatctaaac agatctcagc aaaccctgca ttctccgcaa gaaatccata
     2761 tcccggatgt attgcatctg cattagtgat cgatgctgct gacattattc ttggaatgtt
     2821 taaatagctt tcctttgaaa gtggtgaacc aatacaaact gcttcgtctg caaattttac
     2881 atgcagagaa tttttatctg catctgaata aacagctact gtcttaatat ccatctcttt
     2941 acaggtcctg ataattctta aagctatttc acctctgttt gctattaata atttttttat
     3001 catttatgtt tcttctatgt ttcttcattt taaaaaaagg gaaggcttgc aatactggaa
     3061 aatttattct atctctatca aaaacaacgg ttggttatat tccaccggtt gagcgttttc
     3121 tacaagtatt tttataattt ttcctgaaac ttctgattca atttcattca taagtttcat
     3181 tgcttcaatg atacaaagaa ctgaaccttc agaaacggat tttccgacac ttacatatga
     3241 atctgcatcg ggagaaggtg atctgtaaaa tgtaccaacc attggggagt ttacttcgac
     3301 taatttacta ttttttaaag tctcggtctg cggcatttcc tgaatgttct gatcagactt
     3361 tgattcagat tcctgctgct gcattgcagg cataaaattc attgaagaaa tatcaaatgg
     3421 ctgtgatgcc attgctatct tattttttgg ttttgacaat cttaatctgg atccttcttc
     3481 ctgtacttca aattcagaaa tgttgctcga atcaagaatt tttataagtt tctttaaata
     3541 gttaagatct aatttcataa tgtttttaaa atttattgat tataatttgg ctctttcaat
     3601 atagtcgcct gttcttgaat ctacttttag cacatcacct tcattaataa ataaaggtgc
     3661 gtttacagtt gctccggttt ctaattctac aggcttagtt gctcctgttg cagtatctcc
     3721 ttttactcca ggaggagctg aaattacttt cagaaaaaca tgcgggggaa tttcgattga
     3781 cagcggatta gaattatgaa acatgatctg tacaatctca ccttctttaa gataattccc
     3841 accctttcca ataatatctc cggcaattgt tacttgttca aaagtctcat tttccataaa
     3901 cacataatct tcaccgtctt tgtataaata ctgataattt ccggtatcca gtctttcaag
     3961 ctgaatagat tcaccggatc ggaatctgtt ttccattgtc ttgccgtttt tcagattttt
     4021 taatttaacc tgataaaaag cgcgaagatt acccggagtt ctgtgctcta cggaaataac
     4081 tgaatgtagt tcattattaa aattaattat aacaccgttt tttagatctg aggttgaagc
     4141 catttattat tttataaata ttttattcga aaaatttata caaaatagct aattaaccat
     4201 ttaaaaacaa tttacagttt caaaccgttt ttcatcattt tctataaaaa taattaaatt
     4261 tattgaagtt ttttacaaat ttttataaaa aactgttaat tttttaaaat taattcaccc
     4321 gagttctgca ttatttgctt tcaaaatcaa agaaagttgt ccaatatgca tcggttcgtg
     4381 tctgattgca tgcataatca aagttttttt tgattttgaa ccgccgattg tgaaattaaa
     4441 ataattatct ttttctaaat ct
//
LOCUS       DAORVQ010000239         4429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_14635_length_4429_cov_6.308185, whole genome shotgun sequence.
ACCESSION   DAORVQ010000239 DAORVQ010000000
VERSION     DAORVQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_4429_cov_6.308185"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1975
                     /gene="acs"
                     /locus_tag="PLX80_13375"
     CDS             23..1975
                     /gene="acs"
                     /locus_tag="PLX80_13375"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQY21826.1"
                     /translation="MAEKNITSLSRENRLFKPKKSFSEKAYIKSFQQYKRAYEKSIKD
                     PEKFWSKIADELHWFKKWKKVLKWKAPHAQWFTGGKINLSYNCLDRHLETHRKNKVAL
                     FFEGENGDTQTFTYSQLAAEVRKFSNVLKNNGIKKGDRVVIYMPMIPEAVIAMLSCAR
                     IGAVHSVIFGGFSANALVDRITDAEAALVITADGANRRGQIVHLKNNVDEALPNCPTV
                     KNVIVIKRCGCDIQWNEYYDKWYHEEMADVKDICPAEKLDSEHPLYILYTSGTTGKPK
                     GILHTTGGYSVHTYITTKYIFDIKDEDIFWCTADIGWVTGHSYVVYGPLQNGATVFIY
                     EGAPNFPHPDRFWQLIDKYKITIFYTAPTAIRAFIKWGNHWVDKYSLRSLRLLGTVGE
                     PINPEAWMWYNKMIGKEKCPIVDTWWQTETGGIMIAPFPGGTPTKPGSGTLPFFGIEA
                     EVVDKKGKKVEKGSGGLLVIKKPWPGMLRTIYKDDERYKQQYWSEFPGIYFTGDGARR
                     DKDGYFWVMGRVDDVLNVSGHRLGTAEIESALVAHMKVAEAAVVGKPDEIKGNSICAF
                     VTLEGSQIPTDELKQELKDWVVKEIGALARPDEIRFSDSLPKTRSGKIMRRLLRELAH
                     SGEVKGDVTTLEDYSVLEKLRAEEEE"
     gene            2154..2591
                     /locus_tag="PLX80_13380"
     CDS             2154..2591
                     /locus_tag="PLX80_13380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21827.1"
                     /translation="MKKVRDNEFNFIEMESSHFPLLKEFWENTDGIKLTIGDTESELS
                     NYLKRNKGMSFICMNGADLIGTILCGHDGRRGFIYHLAVHKDFRNDNIAADLIEMSLD
                     QLRAADIKRCMIMTDEINESGKSFWLKMDWRKRDDLKMFSIDL"
     gene            complement(2787..4172)
                     /locus_tag="PLX80_13385"
     CDS             complement(2787..4172)
                     /locus_tag="PLX80_13385"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21828.1"
                     /translation="MKTKLPIYVIPFLALTFLFYSFTTSNNTEVYEVNTVSSVSGIST
                     LVLVHDSTTATVKRDADRDTLRKHLPAAVGSYVMVAFDTNTVLPDLTPFNQIILQETS
                     FDVANRRYLGAGARAQIKAWLNTGTSLVRKTLVSIGADQSYNYSRSGSGGRDLDWSEN
                     YFKFIYRVDNKAGSTSPSITGTNVDIGNLRAMFTTVAGGGYWPDGASMATGGIPLYRY
                     QNNSSVGQDTLPVIGHIEAGYVVATMFQDPRYFTEGFGEVLNALVMWVNDNSPLPVEL
                     SSFVSSVNGNDVTLNWTTASEINNSGFDIERSSVENQWSKVANVAGNGTTNESSSYSY
                     TDRSLNAGIYSYRLKQVDFNGNFEYFNLSAEVNVGVPANFKLSQNYPNPFNPSTTIEF
                     ALPTDGHAVITVFDMSGKEVSRLVNEVKSAGYHNVKFDGSTLSSGSYFYKIEVTGTSN
                     FSEVKRMMLVK"
BASE COUNT         1441 a          774 c          875 g         1339 t
ORIGIN      
        1 ttcaaattat ctaatacctc caatggcaga aaaaaatatt acttccttat ccagagaaaa
       61 cagattattc aaaccaaaga aatcattttc tgaaaaagct tatatcaaat cctttcagca
      121 gtataaaagg gcttatgaaa aatccataaa agatcctgaa aaattctggt ccaagatcgc
      181 tgatgaactt cactggttta agaagtggaa aaaagtcctg aagtggaaag ctcctcacgc
      241 gcaatggttt acaggcggga agatcaatct cagctataat tgtctggaca gacatcttga
      301 gacacacaga aaaaataaag ttgcattatt ctttgaaggc gagaacgggg atacccagac
      361 atttacttat agtcagcttg ctgcagaggt aagaaaattt tctaatgtat taaaaaataa
      421 cggaatcaaa aaaggtgaca gggttgtcat ctatatgccg atgatacccg aagcagtgat
      481 agcaatgctt tcctgtgcaa ggatcggagc agttcactcg gtcatattcg gaggattttc
      541 tgcaaatgct ttggtggaca ggatcacgga tgcagaagca gcacttgtaa ttactgctga
      601 cggtgctaac agaagagggc agatcgttca tttaaaaaat aatgtggatg aggcattgcc
      661 gaactgcccg actgtgaaaa atgtgattgt aataaaacgt tgcggctgtg atattcaatg
      721 gaatgaatat tatgataaat ggtatcatga agaaatggca gatgttaaag atatttgtcc
      781 tgcagagaaa cttgattctg aacatccgct atatatcctt tatacaagcg gcactacagg
      841 gaaaccaaaa ggcattcttc acacaaccgg cggttattct gtacatactt acattactac
      901 caagtatatt tttgatataa aagatgaaga catcttctgg tgtactgctg atatcggctg
      961 ggtaacagga catagttatg tagtttacgg acctcttcaa aatggtgcta cagtttttat
     1021 ttatgaaggc gcaccgaatt ttcctcatcc tgacagattc tggcaattga ttgacaaata
     1081 taaaattaca attttctaca ctgcaccaac cgcaatccgt gcatttataa agtggggtaa
     1141 tcactgggtg gataaataca gtctgagatc tctacgctta ctcggcactg taggtgagcc
     1201 tataaatcct gaagcatgga tgtggtataa caaaatgatc ggaaaagaaa aatgcccgat
     1261 agttgacaca tggtggcaga cagaaacagg cggtattatg atcgcacctt ttccgggagg
     1321 tacaccaacc aagcccggat caggaactct tcctttcttt gggatcgaag cggaagttgt
     1381 agataaaaaa ggtaagaaag tagaaaaagg cagcggtggt ttactggtca taaaaaaacc
     1441 atggcccgga atgcttagaa ctatttataa agatgatgaa agatataaac agcagtactg
     1501 gagtgagttt cccggaattt atttcacggg tgatggagcg cgcagagaca aggacggata
     1561 tttctgggtt atgggtaggg tcgatgatgt cctgaatgtc agcggtcaca gacttggaac
     1621 tgccgaaatc gagtcagcac tggtcgctca tatgaaagta gcagaagctg ccgtggttgg
     1681 aaagcctgat gagataaaag gaaattcgat ctgcgctttt gtgactcttg aaggtagtca
     1741 aattccaacg gatgaactta agcaggaact caaagactgg gtcgtcaaag agatcggcgc
     1801 tcttgcaaga cctgatgaaa taagattctc tgattctctt ccaaagaccc gaagcggaaa
     1861 gatcatgaga cgcttacttc gtgagcttgc acacagcggt gaagtaaaag gtgacgttac
     1921 aacacttgag gattattcgg tgcttgaaaa attacgagct gaagaggaag agtagaagga
     1981 tcaattacga atttggaatt aagaactgct aattataagt tacgagttac gaattacacc
     2041 gtttccgcag attcacgtgc ttttcggact ctgcggtgag atttaatttc taacttcgtt
     2101 tctgcagagt caggtgctct acgaactctg cagtgttaaa attaaatata aaaatgaaaa
     2161 aagtccgcga caatgaattt aattttattg aaatggaatc ttctcatttt cctttgctga
     2221 aagaattttg ggaaaatact gacggaataa aacttacaat aggtgataca gaatcagaac
     2281 taagtaatta tcttaaaaga aataaaggaa tgagttttat atgtatgaat ggagccgatc
     2341 tcattgggac gatcctctgc ggacatgatg gtaggagagg attcatttat catctcgctg
     2401 ttcataaaga tttcagaaat gataacatag cagcagatct gattgaaatg agtctggatc
     2461 agcttagagc tgcagatatt aaaaggtgta tgatcatgac tgatgaaata aatgaaagcg
     2521 gtaaatcctt ctggctgaaa atggactggc ggaagaggga tgatctaaag atgttttcta
     2581 tagatctgtg aattttttat tttaaatttc taatccattg gttttccgca gagtccgaag
     2641 agcacgtaac tcagcggaga ctgaattttt ttttaactct ttaatataaa tttttatcat
     2701 aaaaaaaccg gaagcaataa aaacttccgg tttaaaatat aaaaatttgt taatctaaat
     2761 tatccttctt caggataaat caaattttat tttacgagca tcattctttt cacttcagag
     2821 aaattacttg tacctgttac ttcaatctta taaaaataac ttccgcttga aagtgtagat
     2881 ccgtcaaatt ttacattatg atatcctgct gatttcactt catttactaa tcttgaaact
     2941 tctttacctg acatatcaaa tacagtgatc actgcatgtc cgtcagtcgg aagagcaaat
     3001 tcaatagtag tagatggatt gaaaggattc ggatagttct gtgataattt gaaattagcc
     3061 ggaacaccaa cattgacttc agcactcaga ttgaaatatt caaagttacc gttaaaatca
     3121 acttgcttaa gtctgtagct gtatattcct gcatttaagg atctgtcagt atatgaatat
     3181 gaacttgatt catttgtagt tccgtttcct gctacattag caacttttga ccactgattt
     3241 tcaactgaag atctttcaat atcaaatcct gagttattga tctcacttgc tgttgtccag
     3301 ttaagtgtaa cgtcatttcc gttcactgat gaaacaaatg aagatagttc tacaggtaaa
     3361 ggactgttat catttaccca cattaccagt gcatttaaga cttcaccaaa tccctctgta
     3421 aaatatcttg gatcctggaa cattgtagct actacataac ctgcttcaat atgacctatt
     3481 acaggtaatg tatcttgtcc tacagatgaa ttgttttgat atctgtatag aggaatacca
     3541 cctgttgcca tacttgcacc gtccggccag taaccgcctc ctgcgactgt ggtaaacatt
     3601 gctcttagat tgccaatgtc aacgtttgta ccggttattg aaggtgaagt agaaccggct
     3661 ttattgtcaa ctctgtaaat aaacttaaag taattttcgg accaatccag atctctgccg
     3721 ccgcttccgg atctgctgta attataagac tgatctgctc caatacttac taaagtcttt
     3781 cttactaaag atgtaccggt attaagccat gctttgatct gagctcttgc acctgcacca
     3841 agatatctgc ggtttgcaac atcaaatgaa gtttcctgaa gtatgatctg attaaaagga
     3901 gttaaatccg gtaacacagt atttgtatca aatgcaacca taacataact tcctacagct
     3961 gcaggaagat gctttcttaa tgtatccctg tcggcatctc tttttactgt tgcagtggta
     4021 gaatcatgta caagaactaa tgtacttata cctgaaacag atgaaactgt atttacttca
     4081 tacacttcag tattattaga agtagtaaat gagtagaaca aaaatgttaa agctaaaaac
     4141 ggaattacgt agatcggtag ttttgttttc attttacttt cctttcgggt taattaataa
     4201 tatgttaaga gaacagaata gatgctctcc tttttgttac tgataatgtt tttttttgta
     4261 tttttatttt aatgggtttg aattaaaatt tcaagtcttt tttaaaataa aattgcgaaa
     4321 tataaatcat gtctatccaa atcgaatgag aatcatttgt catataatct tcctatttta
     4381 aatgactaat attgacattc aaactacatt aattacaatt cggacaagt
//
LOCUS       DAORVQ010000240         4427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_14645_length_4427_cov_4.462260, whole genome shotgun sequence.
ACCESSION   DAORVQ010000240 DAORVQ010000000
VERSION     DAORVQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14645_length_4427_cov_4.462260"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1763)
                     /locus_tag="PLX80_13390"
     CDS             complement(60..1763)
                     /locus_tag="PLX80_13390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQY21829.1"
                     /translation="MKKLINRFSILLIFTAMLIFSGCSEKPDVMYINGKIYTMNNDNK
                     VVEAVAIKEGKIMETGTTEELTDKYKAEETIDLKGATVLPGFTDSEGSIIEFSKNLNY
                     INLSYAKSLDEIKQLIIERTKIASEGEWIGGYGYNESVFTDEDYAKFDKTFLDQIAPN
                     FNVFIVNGTFSTALLNSRAMRVLTITKDTKAPGNGEIFTDDNGELTGLLFNDAINIVK
                     DNMPGLLKSEMIKRVETGVQEILKYGITEVHDRSIGQEGIEIIKELIDGNRFPLRVYA
                     VLSGEETSLVDLYLNKGIEVGYKDHLTVRAITVDYDGLLINQKAVLFEDYKNEPKKSL
                     PYLTDDEIKNIFNKAADKKFQFSIKAVGDKAVNSSLNIIESVSKEKNLTDSRTVLEFC
                     EVLDAKDISRFGELKVIPSVRPDVTITDLQITEELINAETLKKIGLWNSLLKSSGMLV
                     SASDFPMHQINPFVQLYYLTTRQRTDTLKIQGNNTDQKISLLEAVKSYTVWPAYAAFE
                     ENVKGTIEKGKFADMIVISKDIFNSDSKELLNTKVIRTILSGRIVYEDKNLLSSINDR
                     K"
     gene            1912..2364
                     /locus_tag="PLX80_13395"
     CDS             1912..2364
                     /locus_tag="PLX80_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQY21830.1"
                     /translation="MKKNFAMKKLYYSISEVSRIIELEQYVLRYWETEFELLRPAKNL
                     AGNRVYTNKDIKLILNIKKLLREEKYTIEGAKQHLKNFYQKEEQENTRPVSHTRESNS
                     VRNGSEMSLFEPSTSEPEETENITKNPNLKDDLIQLRSFLTNLLKDIS"
     gene            2468..3607
                     /locus_tag="PLX80_13400"
     CDS             2468..3607
                     /locus_tag="PLX80_13400"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HQY21831.1"
                     /translation="MNLLIAKKDAVKIILNNQLLINSDLNGSKDSILKIISQLGYIQI
                     DTISVTERSVNHIIWSRAGKFERSNINELFLEGKIFEYWSHAAAILPMTDYRYSIIFK
                     NYYIERYGSFKKSNNRLLKLVKDRIKSEGPLMSRDFEDKRTGTSGWWNRKPAKEALEF
                     LFFSGELMVKKRTGFQKVYDLTERVLPSGIDDSIPTKEEYHKHLILSSIKANGIISGK
                     EIAYLKRNDKKVFSEVINELIEDKQVCEITINGVNDINYFTKPELLELKISDRSGLKI
                     LSPFDNLIIQRERIKQIFKFDFLLECYLPQSKRKYGYFSMPVLYNNEFLGRIDMKSDR
                     KIKTLKVNKFYPENETKLSNTQMKKLNSLLQEYAEFTGCEKVEWI"
     gene            complement(3670..4380)
                     /locus_tag="PLX80_13405"
     CDS             complement(3670..4380)
                     /locus_tag="PLX80_13405"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQY21832.1"
                     /translation="MKILYVFPHPDDESFGVAHAMSKQLREGHEVYLLTLTKGGATKQ
                     RFKYNYSVEEMGEVRFNEMKNVEKVLGLTGMTVLDLPDSGMKEMNPEDIEKVVEDEIM
                     KIKPDVIVSYPVHGISGFQDHLVMHGVIKSVFCRLRTEQKFLKRLAFITVTKEDSEVS
                     EFFKLSFSTESEIDCVIETEDSDIQKAIDSLDCYVTFSETIEKSNIKNFIKHKVNFEI
                     FQEDHDPVLKSLFENLNK"
BASE COUNT         1510 a          748 c          622 g         1547 t
ORIGIN      
        1 ccgatacagc tttttttagt atttcaagaa cggttataaa ttgattttta tccgtagtat
       61 tattttcggt catttataga actcaataaa tttttgtctt catatactat cctgccgctc
      121 aaaattgttc tgattacttt tgtatttaaa agttctttac tgtcagaatt gaaaatatct
      181 tttgagatca cgatcatatc tgcaaatttt cctttctcta tagttccttt aacattttct
      241 tcaaatgcag cataggctgg ccaaactgtg tatgatttta cagcttcaag taaacttatt
      301 ttttggtcag tattatttcc ctgtatcttt aatgtgtcag ttctttgtct tgtagtaaga
      361 taatataact gaacaaacgg atttatctgg tgcattggaa aatcagatgc cgatacaagc
      421 atacctgatg attttaatag tgaattccaa agtccaatct tcttaagtgt ctctgcattt
      481 attaactctt cggtgatctg tagatctgta atggtaacat caggtctgac tgaaggaatt
      541 acttttaatt caccaaatct ggatatatct tttgcatcta acacttcaca aaattcaagc
      601 acagtccttg agtctgtcag atttttttct tttgaaacac tttcaataat atttaatgaa
      661 ctgtttacag ctttatcacc aactgctttt atagaaaact gaaatttttt atctgcagct
      721 ttattaaaaa tatttttgat ctcatcgtcc gtcagatacg gaagactttt tttaggttca
      781 tttttataat cttcaaacaa aaccgctttt tgatttatca atagcccgtc ataatcgact
      841 gtaatagctc ttactgttaa atgatcttta taaccgactt caataccttt attcaaataa
      901 agatcgacta atgatgtttc ttctccagat aaaactgcat atactctcaa aggaaatcta
      961 ttaccgtcga tcagctcctt gattatttct attccttcct gccctatact tctgtcatgt
     1021 acttccgtaa ttccatactt taaaatttcc tgtacacctg tctcgacccg ctttatcatt
     1081 tcacttttaa gcagacccgg catattgtct tttacaatat taattgcatc attaaaaaga
     1141 agacccgtaa gttctccatt atcatcagta aatatttcac catttcctgg agcttttgta
     1201 tcctttgtga tagtcaatac tctcatcgct ctgctgttta aaagtgcagt ggaaaaagta
     1261 ccatttacaa taaacacatt aaaatttgga gcaatctgat ccagaaaagt tttatcaaat
     1321 tttgcataat cctcgtcagt aaatactgat tcattatatc catatccgcc aatccattct
     1381 ccttcagatg caattttggt tcgttcaatg attaactgtt ttatctcatc aaggctcttt
     1441 gcatatgaga gatttatata attcagattc tttgaaaact caatgattga accctccgaa
     1501 tcggtaaaac ctggaagtac agttgcacct ttcagatcaa tagtttcttc agctttatat
     1561 ttatcagtca gctcttctgt agtccctgtt tccattatct tgccttcttt aatggcaaca
     1621 gcttccacta ctttattatc gttgttcatt gtatagatct ttccgttaat atacattaca
     1681 tccggctttt cagaacatcc gctaaatatc agcattgccg taaagataag taagattgaa
     1741 aagcgattta ttaatttttt catttatata gttggttttg aattcagtaa aactaactaa
     1801 atcaatcaaa taattaaata cattacataa cctctttaaa gtaaagatat ttaacattgg
     1861 tattgcttaa tataatctat atacatattt ttacgcaaat aattaaattt tatgaaaaag
     1921 aattttgcaa tgaaaaagct ttattattct atcagtgaag taagtagaat aatagaactt
     1981 gagcaatacg ttctgagata ctgggaaaca gaatttgaat tgctcagacc ggctaaaaat
     2041 cttgccggaa acagagtcta tacaaacaaa gatatcaaac tgattctgaa cattaagaaa
     2101 ttgcttcggg aagaaaaata cacaatcgaa ggtgcaaaac aacatttaaa aaatttttat
     2161 cagaaagaag aacaggaaaa tacaagaccg gtttcacata cacgtgaaag taattccgtg
     2221 aggaatggaa gcgaaatgag cttgtttgag ccctcaactt ctgaacctga agaaaccgaa
     2281 aacataacta aaaatcctaa cctcaaagat gatcttatac agttaagatc tttcttaact
     2341 aatctcttaa aagatatatc ctaaccgctt taaagataaa gtgcttatat tcccagtaag
     2401 cactttattt ttaaagtgca gagtccttta attccatttc actaaaattt aagttccgct
     2461 caattcaatg aatttattaa ttgcaaaaaa agatgctgta aaaataattc ttaataatca
     2521 gttattaata aattctgatc tgaatggatc gaaagattca attctaaaaa tcatttcaca
     2581 gcttggttat attcagatag atacaatatc cgttactgaa agatctgtaa atcatataat
     2641 atggtcaaga gccggaaaat ttgaaaggag taatattaat gaattatttc tggagggtaa
     2701 aatatttgaa tactggagtc atgctgcggc tatacttccc atgactgatt acagatattc
     2761 cataattttt aaaaattatt atattgagag atatgggtca tttaaaaaaa gtaataatcg
     2821 tttactgaaa ttagtaaaag acaggataaa atcagaaggt cctctgatgt caagagattt
     2881 tgaagataaa agaactggaa cttcaggttg gtggaacaga aaacctgcga aagaagctct
     2941 tgaattctta ttcttttcag gagaactgat ggttaaaaaa cgcactggtt ttcaaaaagt
     3001 ctatgatctt actgaaagag ttttaccttc aggcattgat gattcaattc caacaaaaga
     3061 agaatatcat aagcatttaa ttttatcttc aatcaaagca aatggaataa tttccggaaa
     3121 agaaatagct tacctgaaaa ggaatgacaa gaaagtattt tctgaagtaa taaatgaatt
     3181 gatagaagat aaacaggtct gtgaaatcac aatcaatgga gtaaatgaca ttaactattt
     3241 tactaaacct gaattactgg aactgaaaat ttccgacagg tcaggattaa aaatactatc
     3301 accatttgat aatctcatca ttcaaagaga aagaattaaa caaattttta aatttgattt
     3361 tctgcttgaa tgttatttgc ctcaatctaa gcggaaatat ggttacttct cgatgcctgt
     3421 actttataac aatgagtttt taggaaggat tgatatgaaa tctgacagaa aaattaagac
     3481 tctaaaagtt aataaatttt atccggagaa tgaaacaaaa ctcagcaaca ctcaaatgaa
     3541 aaaattaaat tctcttttac aagagtatgc agaatttacc ggttgtgaaa aagttgaatg
     3601 gatttaaaat tcattggaat tattatctga aagattaatc cggaatatat atatatttta
     3661 taattatttt tatttattca aattttcaaa caagcttttt aaaaccggat catggtcttc
     3721 ctgaaatatt tcaaaattta ctttatgctt tatgaaattt ttaatattgc ttttttcaat
     3781 agtttcagaa aatgtaacat aacaatctaa tgaatcaata gccttttgaa tatcagaatc
     3841 ctcagtctct atcacacaat caatttcact ttctgttgaa aaactaagtt taaaaaattc
     3901 tgaaacttcc gaatcctctt ttgtcactgt aataaacgct aatcttttga ggaacttttg
     3961 ttcagttctt aaccggcaaa aaactgattt tattactcca tgcattacaa gatgatcctg
     4021 aaatccgctg attccgtgga caggatatga aacgataaca tcaggtttga ttttcataat
     4081 ctcatcttca actacttttt caatatcttc cggattcatt tctttcattc cgctgtcagg
     4141 cagatccaga acagtcatac cggttagtcc taagactttc tcaacatttt tcatttcatt
     4201 gaatcgcact tcacccattt cctcaacgga ataattatat ttgaacctct gtttcgtagc
     4261 accacctttg gtaagtgtga gaagatagac ttcatgtcct tctcttaatt gttttgacat
     4321 agcatgagca actccgaaag attcgtcatc aggatgcgga aatacatata atattttcat
     4381 aataattttt aattaatttt tgtttgtaaa attcaaaatc cagcttt
//
LOCUS       DAORVQ010000241         4418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_14693_length_4418_cov_5.241806, whole genome shotgun sequence.
ACCESSION   DAORVQ010000241 DAORVQ010000000
VERSION     DAORVQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14693_length_4418_cov_5.241806"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3796)
                     /locus_tag="PLX80_13410"
     CDS             complement(<1..3796)
                     /locus_tag="PLX80_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HQY21833.1"
                     /translation="MSDLKDNENIQKLSAQEKLALLKSQLLDKTKKEDVTFQMTYGQK
                     ALYFLYLNTPESTAYNVAFSVRIKSHVNIPALKKSFQRILNRDQCLRSNFKIENGKPV
                     QTVSGFKDIFFRSEDHSEKSEKVIKEIINSYHRTPFDLENGDVFKVYLVKVSDENYIL
                     LISVHHIACDGWSIGTMLNELKQFYYAESDNLQISFSPLQKKYSDYVNFLEDFIKSEK
                     GNEQWNYWKNELKGDLPYLNLTPDKHRPAVQTYNGATEYLFLEKELVDKLKAISKSEG
                     TTLFVTLLTAFFVFLYKYCGQEEIIVGLPTAGRTNTEFDKVIGYFINPVAVRAIVDPE
                     NSIADFLKQVKKKVLYAVANQDYPFPLIVEKILNKRDPSRSPVFQTFFGMQKVQQSDE
                     IQELIVYGNDNAEVNWGSLKLQPYYLTQQDGQFDLTVEFIEGENSFSGAFKYNADIFN
                     KDTIISMKENFITLLKNIVSDSEKSVKDLKILSDAEESKILNQWNDTETLINEQTPVY
                     HSIKKIADLYPDNTAVISDESEISFKELVERSGKLTNYLIRKGVKPKDLICICMERSL
                     DLVISILGILKAGAAYVPVDPSYPQNRIDHMIKDSGSKFILTQRSVSEKLRSDNAEIL
                     IADESNLEIMDENSEVPDINVTLEDPAYAIYTSGSSGIPKGVLISHRSLANHMQWMIN
                     EFGFDSNVCVLQKTPFSFDASVWEFYLPLMTGGKLVMAKPEAHMDTAYMKDLIIKSEV
                     NVLQMVPTLLNLILDEPMIQDCKSLKFIFSGGEALTKTLCEKVFSKLDVTLCNLYGPT
                     EATIDSTFYICNKNETDENIPIGKPVSNAKAYVLDKALNPVPVGVAGELFLGGDNIAL
                     GYVNNISLTDEKFIADIFSNIPERKLYKTGDLAKYDSNGDLIFLGRADDQVKFRGYRI
                     ELGEIETALNSIENINTSVVLVREDKPGIQKLTAYLKLNNDSELEINEYKNELRKSLP
                     EYMVPSAFILLESIPRTPNGKIDKSTLPAPEDSLSAQAGHIEPEKPVEKILANIWKEV
                     LGVENVGIHDNFFELGGDSIISIQIISRANQEGIKISPRQVFQYQTISELSAVAEQKT
                     AEAVDQNPVTGNALLIPIQQRFFQHNIKDPSHFNHSILLSLPDNINTEHLEKAVKEIL
                     IHHDALRMSYEIIGNNLTQINNYIGNDIPFIVEDLTSLDPDNIDNKINELTINYHKNI
                     DISKGKLFNAVLFRTGNNSGNKLLIIVHHLCIDGISWRIILEDLYRLYNNFQNNEDPK
                     LPF"
     gene            complement(3824..>4418)
                     /locus_tag="PLX80_13415"
     CDS             complement(3824..>4418)
                     /locus_tag="PLX80_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQY21834.1"
                     /translation="IDPERGIKALEKIFNCGNAQIGILPMDWNKYFESTKNKFTVKLI
                     SELRDPNEEISKKDDDDFFDRLRNANESEQTELLKDYFRDLISMIMGLDPEDLDDEQP
                     LNTMGLDSLMAIELKNKVNIELGVDLNLVRYMEETGIIHLAAELKEQLPKILNNSSKE
                     TVKTVSEISEEEKARDLLANLDDLSEEELDRLLNESK"
BASE COUNT         1335 a          795 c          718 g         1570 t
ORIGIN      
        1 taaacggcaa ttttggatct tcattgtttt ggaaattatt ataaagtctg taaagatcct
       61 ccaatataat ccgccatgaa ataccgtcga tacataagtg atgaacaatt atcagaagtt
      121 tattaccgga attatttcct gtcctgaaaa gaactgcatt aaataatttc ccttttgaga
      181 tgtcgatgtt tttgtgataa ttaattgtca gctcatttat tttattatca atattgtcag
      241 gatccaatga agtcagatct tctacgataa aaggaatgtc attgccaatg taattattga
      301 tttgagttag attattccca attatttcat aactcattct aagagcatcg tgatggatga
      361 gaatttcttt tactgctttt tcgagatgtt cagtattaat attatcaggc agagacagta
      421 aaatagaatg attgaaatgg gatggatctt ttatattgtg ttgaaaaaat ctttgctgaa
      481 tgggaatgag cagagcattt cctgtgacgg ggttttggtc aaccgcttca gcggtttttt
      541 gctcagcgac agcggaaagt tcagagattg tctgatactg aaagacctga cgcggactta
      601 tctttatacc ctcctgatta gcacgggaaa taatctgaat gctgataatg gaatcaccgc
      661 caagttcaaa aaaattatca tggatgccaa cattctctac tcctaaaact tctttccaga
      721 tatttgccag gatcttctca acaggctttt caggttctat gtgtcctgct tgtgcagata
      781 aagaatcttc cggagctggc aaagtagatt tgtcaatttt tccatttgga gtgcgtggaa
      841 tactttcaag taatatgaaa gctgaaggta ccatatattc aggtaaactt ttccttaatt
      901 catttttgta ttcatttatt tctaattcag aatcattatt caatttcaag taagctgtca
      961 gcttttgaat acccggttta tcttctctta ctaatactac tgaggtgtta atattttcaa
     1021 ttgaattaag agctgtttct atttctccca gctctatcct gtatcctctg aatttaacct
     1081 gatcatctgc tcttccaaga aaaattaaat ccccattaga atcatatttt gccaggtcac
     1141 ctgttttata taatttcctt tcgggaatat tagaaaaaat atcagctata aatttttcat
     1201 cagtaaggga aatattatta acataaccta acgcaatatt atctccacca agaaacaact
     1261 ctcctgcaac tccaacaggt acgggattca aggctttatc gaggacatat gcttttgcat
     1321 tactcacagg cttaccaatc ggaatgttct cgtcagtttc atttttattg caaatataaa
     1381 atgtagaatc tatagttgct tcagtcggtc cataaagatt gcataaagtt acgtcaagtt
     1441 ttgaaaaaac tttttcacaa agagtttttg ttaaagcctc tcctccggaa aatataaatt
     1501 taagactttt gcaatcctga atcattggtt catccaatat cagatttaaa agcgtaggaa
     1561 ccatctgtag tacattaact tcgcttttta taattaaatc tttcatgtaa gcagtgtcca
     1621 tgtgagcttc aggcttagcc ataacaagtt ttcctcctgt cataagcggc agataaaatt
     1681 cccatacaga ggcatcaaaa ctgaacggag ttttttgcaa tacacaaaca tttgaatcaa
     1741 atccaaattc attaatcatc cactgcatat ggtttgccag agatctgtga ctaatcaaaa
     1801 caccttttgg aataccggat gaacctgatg tataaattgc ataagcagga tcttcaagag
     1861 ttacatttat atcgggtact tcagaatttt catccataat ttccaaatta ctttcatcgg
     1921 caattaggat ctctgcatta tctgatcgga gtttttctga aacagatctt tgtgtcaaaa
     1981 taaatttaga gcccgaatct tttatcatat gatcgatacg gttctgagga tatgaaggat
     2041 cgaccggaac gtaagcagcg cctgctttta atataccaag aattgatatt accaaatcca
     2101 aagatctttc catacagatg catattagat ctttaggctt tactcctttt ctaatgagat
     2161 aatttgtcag cttgccggaa cgttcaacaa gttctttaaa tgaaatttca gattcatctg
     2221 aaataactgc agtattatct ggatataaat cggcaatttt tttgatcgaa tggtaaactg
     2281 gagtctgctc attaattaaa gtttctgtgt cattccattg attcagtatt ttactttctt
     2341 ctgcatctga aagaatttta agatccttca cagatttttc agaatccgaa actatatttt
     2401 tcagcaatgt gatgaaattt tctttcatcg aaattatagt atccttatta aaaatatctg
     2461 cattatattt gaatgctcct gagaaagaat tttcaccttc aataaattca actgtaagat
     2521 caaactgacc atcctgttga gtgagataat aaggttgtaa tttcaggctt ccccagttta
     2581 cttctgcatt atcatttccg taaacaataa gctcctgaat ttcgtcagac tgctgaactt
     2641 tctgcattcc aaaaaaagtc tgaaatacag gagatctgct cggatctctt ttattcaaaa
     2701 tcttttcaac aattaaaggg aatgggtaat cctgattggc tacggcgtaa agaacttttt
     2761 tctttacctg ctttaaaaaa tccgcaatag aattttcggg atcgactata gctcttacag
     2821 caaccggatt tataaaatat ccaattactt tatcaaattc ggtattggtt ctaccggctg
     2881 taggcaatcc gactatgatc tcttcctgac cgcagtattt ataaagaaaa acaaaaaaag
     2941 cagtaagcaa agttacaaaa agagttgtgc cttctgattt ggaaattgct ttaagtttgt
     3001 ctaccagttc tttttcaaga aataaatatt cagtcgcacc attataagtc tgtactgccg
     3061 gtctatgttt gtccggtgtt aaatttagat aaggtagatc gcctttaagt tcatttttcc
     3121 agtaattcca ttgttcatta cctttttcac ttttaataaa atcttccaga aaatttacgt
     3181 aatctgaata ttttttttgt aagggtgaaa atgagatttg caggttatcg gattctgcat
     3241 agtaaaattg ctttagttca ttcagcattg taccgatgga ccaaccatca caggcaatat
     3301 gatgaacgct gataagcagt atataatttt catcagatac ctttaccaaa tatactttaa
     3361 atacgtcacc gttttcgaga tcaaatggag ttctgtgata agaattaatt atttctttaa
     3421 taactttttc acttttttcg gaatgatctt ccgatctgaa aaaaatatcc ttaaacccgc
     3481 ttacagtctg aacaggttta ccgttttcta ttttgaaatt agatctcagg cactgatctc
     3541 tgttcagaat tctttgaaat gattttttta aagcaggaat atttacatga gatttaattc
     3601 tgacagaaaa tgcgacatta taagcagtgc tttccggagt gttcagatag aggaaataca
     3661 gagctttctg tccgtaagtc atttgaaagg ttacgtcttc tttttttgtt ttatcgagaa
     3721 gctgactttt aagcagagca agtttttcct gagcagataa tttttgtata ttttcgttgt
     3781 ctttcagatc agacacaata tttacttaat tttaatgagt taattatttt gattcattta
     3841 aaagtctgtc aagttcttct tcggatagat catcgagatt agcaaggagg tcgcgtgctt
     3901 tttcttcttc ggaaatttcg gaaacggttt taaccgtttc tttgctgctg ttgttaagaa
     3961 tctttggcag ctgttctttt aattctgcag caagatggat gataccggtt tcttccatat
     4021 accgtacgag attcagatct actcccagct caatgttgac tttgtttttt aattcgatcg
     4081 ccataagaga atccagcccc attgtattaa gcggctgttc atcatcgaga tcttcaggat
     4141 caagacccat tatcattgat atcaaatccc ggaaataatc tttcagcaat tcagtttgct
     4201 cagattcatt cgcatttcgt aatctatcga aaaaatcatc atcatctttt tttgaaattt
     4261 cttcgttcgg atcccgaagt tctgaaatta attttaccgt aaatttattt ttggtgcttt
     4321 caaaatattt attccaatcc attggcagta ttccgatctg tgcatttccg caattaaaga
     4381 ttttttctaa tgcttttatg cctctctcag gatctatc
//
LOCUS       DAORVQ010000242         4416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_14706_length_4416_cov_5.262784, whole genome shotgun sequence.
ACCESSION   DAORVQ010000242 DAORVQ010000000
VERSION     DAORVQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14706_length_4416_cov_5.262784"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..1411
                     /locus_tag="PLX80_13420"
     CDS             431..1411
                     /locus_tag="PLX80_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HQY21835.1"
                     /translation="MESKTENEIKERIADKSDMVQIPGGTFLMGSDNFYPEERPVRKV
                     TVDGFLMDKYEVTNEQFKKFIDETGYVTVAERPLKQEDYPGVPADKLAPGALIFKKSN
                     GPVDLSNYHNWWVWMPGACWNHPKGPNSSVDSRMKFPVVHVAYEDAETFAKWAGKELP
                     TEAEWEFAARGGLDGKNYIWGDEDIQYSKPEANTWQGEFPYQNLLVDEYEGSSPVGTF
                     APNGYGLYDMAGNVWEWTCDWYLINLTDENAEKFKSCCTPDEKNPRVISPEGSFDECQ
                     PNVKIPRKVVKGGSHLCAPNYCLRYRPAARQPQMIDTGMTHIGIRCIVRK"
     gene            1440..2357
                     /locus_tag="PLX80_13425"
     CDS             1440..2357
                     /locus_tag="PLX80_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQY21836.1"
                     /translation="MRFKLFFSIVNISLIFFLSQHFNHLQAQELEPRNYAVIPTGMNV
                     TALNYTYSSGNIISQATSPIKGLTLISNSFSAGYVRSFSVFGMLSKIQIGVPFIFLRG
                     TAKLADKDSSGTRTGFGDSRIRFGINLLGSPALNPQEFVSHKEDFVLGASIVASVPTG
                     QYSIEKLINLGSNRWGFKPEIGMSYKYSSFYFELYTGIWMYTKNSEYLINKTVEQQPL
                     FVFQSHISYIFPSKIWIAADIGYANGGETEVNGISADNVQNNIRSGFTISVPIAKGHS
                     VKGLFNTGVATRIGGDFTTFTLAYQYSWF"
     gene            2517..4202
                     /locus_tag="PLX80_13430"
     CDS             2517..4202
                     /locus_tag="PLX80_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQY21837.1"
                     /translation="MTKSFQTKLTCWLPGLQIFGNYKSQWLSKDLAAGLSVAAIALPV
                     GIAYASLAGLPTISGLYSCILPMIAYALFGTSRQLMVGPDSATCILIASTLAPLAAAG
                     SAHYQSISVLLTIIVGVMCVIAGIAKFGFIANFLSKPILTGYINGLALSIIASQLGKL
                     NGYNTESGGFFKVVYEYFSNIEKTNMESILIGITTFVFLRVFKKYFNKIPAPLFAAIG
                     GIAVVMIFGLANKGVQIVGEVPSGFPTIILPEVQFGELGALISHSLGIVLISYCSAML
                     TDKSFAIKNNYKIDANKEFIALGAANIFSGLSQGFVISGADSRTAVNDSAGGKTQLVS
                     VVAALTILMVVLFLTGYLTFLPVSTLSAIIISAAIGLFNLKYLKKLYYVSRSEFVLAV
                     ITSLSVITIGVLPGVLIALGITLIRLIAKVSKPHGVILGKMDGYRNYMDISEYKDAQT
                     FPGILVYRFEESLLFFNVDYFRNDVTSIIKKEKIKYLVLDASAILRIDITAADILEDM
                     MCELSEKGVTTVFTKMKPHVEQLLERSGAIKPEDKKKLFESVHTAVDYCKTLK"
BASE COUNT         1462 a          675 c          831 g         1448 t
ORIGIN      
        1 tacatttaaa atttccaagt tatataatca atatatatgc ttgtctgatc taaaacattt
       61 tctgaaataa aaatgaagta ttttaaagaa attataaagt caccatactc agcaagaaaa
      121 ctccgataat aaaaccaaac agttacattc tgatcttgga tcttttatta gtaattcttt
      181 aaaaaataat tgaaataata atttatatct tagaattaaa ttgaattatg agatgtgtca
      241 attacaagtt taattcaatt tatatagagt tgaaatccta tgctaagata gttattttgt
      301 ttcccaaaat actccttatt agtatgtttc aagtggttga ataacttgta aacatttgat
      361 gtccattatg aggaatttat tattgggaat aaaatagttt ttaaagatcc aaaaattaat
      421 tattataaaa atggaaagta aaactgaaaa tgaaataaag gaaagaattg ctgataaaag
      481 cgatatggtg cagattcccg gaggtacatt tttaatggga tctgataatt tttatcctga
      541 agaaagacct gtcaggaaag ttactgttga tggattcctg atggataaat atgaagttac
      601 gaatgagcag tttaaaaaat tcatagatga aaccggttat gtaacagtag cagagagacc
      661 attaaaacag gaagattatc ccggagtacc cgctgataaa ctcgcccccg gagcattgat
      721 atttaaaaaa tcaaatggac ctgtcgatct gagtaattat cataactggt gggtttggat
      781 gccgggtgca tgctggaatc atcctaaagg acctaacagt tctgtagaca gccgaatgaa
      841 atttccagta gttcatgttg catatgaaga tgctgaaact tttgctaaat gggcaggtaa
      901 agaactgccg actgaagctg aatgggagtt tgctgcaaga ggcggacttg atggaaagaa
      961 ttatatctgg ggtgatgagg atattcaata ttcaaaaccg gaagctaaca catggcaggg
     1021 tgaatttcca tatcagaatt tattggtaga tgaatatgaa ggcagttctc ctgtaggcac
     1081 ttttgcgccg aacggatacg gactttatga tatggcaggt aatgtatggg aatggacctg
     1141 cgactggtac ttaataaatc tgacagatga gaatgcagaa aaatttaaat cctgttgcac
     1201 accagatgaa aaaaatccta gagtaatatc tcctgaaggc agtttcgatg aatgtcagcc
     1261 aaatgtgaag atccccagaa aagtagtaaa aggcggttct catttatgtg ctccaaatta
     1321 ctgtctcaga tacagacctg ctgccagaca gcctcagatg attgatacag gaatgacgca
     1381 tatcggcatc agatgtattg taagaaaata aattatttga aaatagatta tatacttaaa
     1441 tgagatttaa actttttttt agtattgtaa atatctcctt aatttttttt ttaagtcagc
     1501 attttaatca tttacaagct caggaactgg agcccagaaa ctatgctgta attccaaccg
     1561 gtatgaatgt tactgcttta aattatactt actcttcggg aaatataatt tcacaagcta
     1621 cctcgccgat taagggactt accttgattt ctaattcatt ctcagccggt tatgtaagat
     1681 cattctctgt gtttggtatg ttgagcaaaa ttcagattgg tgttcctttt atatttttaa
     1741 gagggacagc taagcttgcc gataaagatt caagtggaac cagaacagga tttggggatt
     1801 caagaattag attcggaatt aaccttctcg gatctcccgc actgaatcct caggagtttg
     1861 tatcacataa ggaagacttt gtattaggtg caagcatagt agcttcagtg cctaccggtc
     1921 aatattcaat agaaaaatta attaatttag gatcaaaccg ttggggattt aaacctgaaa
     1981 ttggtatgtc ctataaatac agttcttttt attttgaatt atatacggga atatggatgt
     2041 atacaaaaaa ttccgaatat ttaataaata aaacagttga acaacaaccc ctatttgtct
     2101 tccagtcaca cattagttat attttcccat cgaaaatatg gatagctgca gatattggtt
     2161 atgctaatgg aggagagact gaagtaaacg gaatttccgc tgataatgta cagaataata
     2221 taagaagtgg ttttacaatt tcagtgccaa ttgccaaagg tcattcggta aaaggattat
     2281 tcaataccgg tgtggctacc agaatagggg gagactttac tacattcaca cttgcttatc
     2341 aatattcatg gttttagtta ataaattctt tagcaatata atctgaaaca tttcatcaat
     2401 accggtttgt tttagttcag ccgttttata aatataaaaa tacagttcaa tcaatttatt
     2461 gacatcatca tctttcatga actttaagaa atgatctcta aaaaagtatt tacattttga
     2521 caaagtcatt tcaaactaaa ttaacttgct ggttacccgg gcttcagatt tttggaaatt
     2581 ataaaagtca gtggctttca aaagatctgg cagcaggttt gtctgttgct gcgattgcat
     2641 tgccggtagg aattgcgtat gcaagtcttg ccggacttcc tactatttcc ggattatact
     2701 cttgtatact tccaatgatc gcttatgctt tgtttggtac ttccaggcag ctgatggtag
     2761 gaccggattc agcaacctgc attttaattg cttccactct cgcccctctg gctgctgccg
     2821 gttcagcaca ttatcaatct atatctgtat tattaacaat tattgtcggc gtgatgtgcg
     2881 tgatcgcggg aatagcaaaa ttcggattta ttgcaaattt tctttcaaag ccgatcctga
     2941 caggatacat taacgggctg gcattaagta tcattgcaag tcagcttgga aagttaaatg
     3001 gttacaatac tgagtcggga ggatttttta aagtagtata tgaatacttt tcaaatattg
     3061 aaaaaaccaa tatggaatct attcttatag ggatcacaac atttgttttc ttaagagttt
     3121 tcaaaaaata tttcaataaa attccggcac cacttttcgc tgcaattggc ggaatagcag
     3181 tggtgatgat atttggactt gccaataaag gagttcagat cgtaggtgaa gtaccttccg
     3241 gatttcctac aattatttta cctgaagttc agtttggaga acttggtgct ttgatctcgc
     3301 attctcttgg tatagtgctg atcagttact gcagtgcaat gcttacggat aaaagttttg
     3361 caataaaaaa taattacaaa attgatgcta ataaagaatt tatagctctt ggtgctgcaa
     3421 atattttctc aggactttct cagggatttg taatcagcgg ggcagattcc agaactgctg
     3481 tgaacgattc tgcaggtggt aagacacaac ttgtatctgt tgtcgcagct ttgacgatat
     3541 taatggttgt tcttttttta accggttatc ttacttttct tcctgtttct actttaagtg
     3601 caataataat ttctgcagct attggccttt tcaatcttaa atatttgaaa aaactttatt
     3661 atgtaagccg tagtgaattt gtactggcag tcattacttc attgagcgtt ataaccatcg
     3721 gagtattacc gggtgtattg atagctctgg gtattacttt aataagactg attgcaaaag
     3781 tatcaaaacc acacggtgta atacttggaa aaatggatgg atacagaaat tatatggata
     3841 taagtgaata caaggatgct caaacattcc cgggaatttt ggtctacaga tttgaagaat
     3901 cgcttctttt ctttaatgta gattatttca ggaatgatgt aacttcaata ataaaaaaag
     3961 aaaaaattaa atatctggtt ttggatgcaa gtgcgattct aagaattgat ataacagctg
     4021 cggacattct tgaggatatg atgtgtgaac tttcagaaaa aggagttact actgtattta
     4081 caaaaatgaa accgcacgtc gagcagcttt tggaaagatc cggtgcaata aaaccggaag
     4141 ataaaaaaaa gttgtttgag tcagttcata ctgcagttga ttattgcaag accctaaaat
     4201 aatggtccag attagaaaaa tagaatctcc tgaaattttt ttgttttccc gtatttttat
     4261 ttgattaaat ataaactgat ctttaaaaat gatttatctg atatgaaaat gaaaatgttt
     4321 ctttcacaaa atattattaa aactaaatta ttaaaagtta actaaccggt aaaataacat
     4381 tatttagtat tgaatattaa tattcaggta ttttat
//
LOCUS       DAORVQ010000243         4356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_15084_length_4356_cov_3.952337, whole genome shotgun sequence.
ACCESSION   DAORVQ010000243 DAORVQ010000000
VERSION     DAORVQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15084_length_4356_cov_3.952337"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLX80_13435"
     CDS             complement(<1..292)
                     /locus_tag="PLX80_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQY21838.1"
                     /translation="MREGDIVKAALNQIDNKVKFRPALLLKQMPPFDDWLVCGITSQL
                     SNRVEKFDLLLSENDSEFPKTGLLKTSIIRLGFIAVIPESIIEGIIGSISKNK"
     gene            complement(289..537)
                     /locus_tag="PLX80_13440"
     CDS             complement(289..537)
                     /locus_tag="PLX80_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21839.1"
                     /translation="MDAIRQKVKRTGNKVIVELPDDLKAETFELILFPIEDSPGENSE
                     ESNEWKKFSVRNLERLYETEETDYTNVLVKEPNIKFKP"
     gene            complement(763..1662)
                     /locus_tag="PLX80_13445"
     CDS             complement(763..1662)
                     /locus_tag="PLX80_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21840.1"
                     /translation="MKKLIFKLLIFSLLILNSPSYSQDTVSAKYFPLNVGNTWVYYVN
                     TFPPLENPIKVSSITKDTLINGHKYFFYNSFRFSYEWIRYDSLTGNLIFYSGTENCSV
                     YDNDKIIDSLFSNVGDIIYCDNFAIFTRECTMVSSNIVYNELRSVKRFDHDGLVFHNI
                     EYTEDIGLTRVAFGEPPPNNNNYDQLLGCVIDGVVYGDTTLTSIHQIGNSVPAKFNLF
                     QNFPNPFNPNTVISYDLQSTGFAKIRVFDVLGNEVATLVNEKQSAGSYTVDFDGSNFS
                     SGVYFYRLDTGDFSDTKQMILIK"
     gene            2598..3248
                     /locus_tag="PLX80_13450"
     CDS             2598..3248
                     /locus_tag="PLX80_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21841.1"
                     /translation="MKRKFFDNSMKNFTSQNLFYFLDIHRINIPGFSTSSKYLIFSLR
                     GHFIKKNDLRRSFRDFRRFVKEIPEKVTALRKSFPELREIVTELRESFPELREKVTAL
                     RESFNCLCEKVTELRESFPGLRKFVKDFLKKFKDFRKNVTDFPELFPGSKKRLSGSRK
                     KPSDCGIIMTGSRVSFLHSENYKQVRKINYTGTGSVYFKKYECIRDPNNVPRAPPL"
     gene            3369..3983
                     /locus_tag="PLX80_13455"
     CDS             3369..3983
                     /locus_tag="PLX80_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21842.1"
                     /translation="MKKYYIPDSAGDLDAFEENLVSKLAVHAPALGLDMDEVAQVTQI
                     INDHRAAFIKMISKKAESKSATENNNNSRDMALGEIRRMAQQIKSAKTYTTGIGVDLG
                     IIGSDKSINVTSQLKPALKATVDGHEVVIKFRKEGTDGIKIYGRRGSEQEFTFLAVDT
                     SSPYNDTREKIESGKPEQREYYAYYFEADSEIGQASDVLKVVIP"
BASE COUNT         1491 a          754 c          701 g         1410 t
ORIGIN      
        1 atttattttt actaatactt ccaattattc cctcgataat actctcaggt ataactgcaa
       61 taaaccccaa tcgaattatg ctagtcttta acaatccagt ttttggaaat tcagaatcat
      121 tttcacttaa tagtaaatca aatttttcaa ctctgttact taactgtgaa gtgattccgc
      181 aaaccagcca atcatcaaaa ggcggcattt gctttaacaa tagagcaggt cggaatttta
      241 ctttattgtc tatttgattc aaagcagctt taactatatc gccttccctc atggtttgaa
      301 ttttatattc ggctccttta ctaatacatt agtataatca gtttcctcag tttcatataa
      361 tctttcaaga tttcgaactg aaaacttctt ccattcattt gattcctccg aattttctcc
      421 cgggctgtct tcaattggaa ataaaattaa ctcaaatgtc tcggctttta gatcatcggg
      481 taattcgacg ataactttat tgcctgttcg ctttactttt tgtcttattg catccatatt
      541 aaattaattt tatttaagaa aatcaatatc gttaataaat taaatgattc atttctaaat
      601 taatattact ttttctatct gacagttgta cagtatgtaa ttctggtaat caaaaaaatc
      661 aagcgattca aggttcagac aatattgtcg gctaagattg cagtagtaat atttatattt
      721 taaaatatta aatcagcagc ggttaaaaat attcacctat cattatttta tcaagatcat
      781 ctgcttcgta tcactaaaat ctcccgtatc taatctgtaa aaataaaccc cgctcgaaaa
      841 attacttccg tcaaaatcaa ccgtgtaact tcctgcactt tgcttttcat taacaagtgt
      901 cgcaacttca ttgccaagta catcaaacac tcttatcttt gcaaaacctg tcgattgaag
      961 atcgtaactt attactgtat taggattaaa gggatttgga aagttttgaa ataaattgaa
     1021 ttttgcggga acagaattac cgatctgatg aatagaagtt aaagtagtat caccgtaaac
     1081 aacaccgtca attacacatc ctaacagctg gtcataatta ttattattcg gtggaggttc
     1141 gccgaaagct acccttgtta atccaatgtc ctctgtatat tcgatattat gaaacacaag
     1201 tccgtcatga tcaaatcttt taactgatct caattcatta taaacaatat tacttgaaac
     1261 cattgtacac tcacgtgtaa aaattgcaaa attatcgcaa taaattatat cgcctacatt
     1321 tgaaaaaaga ctgtctataa ttttatcatt atcataaact gaacagtttt ccgttcctga
     1381 ataaaatatt aaattacctg ttaacgaatc ataccttatc cattcataag aaaatctaaa
     1441 gctattgtaa aagaaatact tgtgaccgtt tattaaggta tcttttgtta tagaacttac
     1501 tttaattggg ttttccaaag gaggaaatgt gttaacataa taaacccagg tatttccgac
     1561 atttagagga aagtattttg cagaaacagt atcctgcgaa taggaaggag aatttaatat
     1621 taataatgag aaaataagaa gtttgaaaat taattttttc atgatcttaa atttaattta
     1681 tttaatattt ggagttcata ttgttgttcc aaaaagttaa tatttcttta ttgttggtca
     1741 ttcctgcatt caataataat aaaagttgat ataaatccta tcgtaaatcc agaattcttc
     1801 tcgtaaaata aagatttcct tttcaatata aattaaatta tcagatgata ttttacaaga
     1861 gattaattta aattgagaag tacgaagcag attctgattg tctgaaccat gattcacgtg
     1921 atggtctgaa ccatgattcg cttgattaaa ggactacttg aaacaatagc actattaaaa
     1981 atagatttta aattactcat tcatgtaatc ctaaaaatcc tttaatcaag attcagactg
     2041 tctgaacaat gcttcacgga ctgtctgaac aatgattcac ttgattaaag gattacttga
     2101 aattcattgc acgaaataaa atgtaattaa attattcatc aagtaatcct aaaaatcctt
     2161 taatcaaggt tcagacgcac tgggatggtc tgaaccttga ttcgcttgat taaaggatta
     2221 catgaaataa ttagcacgat ttaaattgat attaaattac tcattcatgt aatcctaaaa
     2281 atccttaaat caaggttcgg acaatccgaa aattctcatt tccaaaaata atttttttca
     2341 tccaatctcc acaaaccctt taaacacaag ccctcaagac acgtcaaaat ccatattttc
     2401 acaaactgcc ttttttacca tttgactcgt ctgcatattt ttacaatatt tgtaggaatt
     2461 tttgaatatg tattgattta tgaacagctt tgaattgtac ttctgatcca atactcagat
     2521 gatccaattc agcagaacgt aaaaatcagg agagtcactc aggataaaat tactttttaa
     2581 aaaagattaa ggtaatcatg aaaagaaaat tttttgataa cagtatgaaa aacttcactt
     2641 cacaaaattt attttacttt ttagatattc acaggataaa tattcccggt ttcagtacat
     2701 cttcaaaata tctcattttt tcactccggg ggcattttat taaaaaaaat gatctccgta
     2761 gatctttcag ggatttccgc aggtttgtca aggaaatccc tgagaaagtt acggcactcc
     2821 gtaaatcatt cccggaactc cgtgagatag ttacggaact ccgtgagtca ttcccggaac
     2881 tccgggagaa agttacagca ctccgtgagt cattcaattg tctctgtgag aaagttacgg
     2941 aactccgtga gtcattcccc ggtctccgta agtttgtaaa ggacttcctt aagaaattca
     3001 aggatttccg taagaatgtt acggatttcc ctgagttatt cccgggatca aagaaaagac
     3061 tttccggctc ccggaaaaaa ccgtctgatt gcggtattat tatgaccggg agcagggtgt
     3121 catttcttca ttcagaaaat tataagcagg tcagaaaaat aaattatacc ggtaccggtt
     3181 cagtttattt taaaaaatat gaatgcattc gggatccgaa taacgttcca agggctcctc
     3241 cgttgtgaag tttcggaccg gaaaaaataa attccgaaga ttaatactgc aggattaaga
     3301 ttcgggattt gtaaaaatgt taaagtacga tcaaaaaatt attattaaaa aattaaaaaa
     3361 ttacaaaaat gaaaaaatat tatataccgg actccgcagg agatctggat gcgtttgaag
     3421 aaaaccttgt cagtaagctt gcagttcatg ctcctgctct cgggctggac atggatgaag
     3481 tggcacaggt tacgcagata ataaatgatc acagagcggc gtttataaaa atgatctcaa
     3541 aaaaagcgga atccaaatcc gctaccgaaa acaacaataa cagccgtgac atggctctcg
     3601 gtgagataag acgcatggct cagcagatca aatcagcaaa aacatatact acaggaatag
     3661 gcgttgatct cggtatcatc ggctcggata aatctataaa tgttacttct cagctgaagc
     3721 cggctctcaa agcaactgtg gatggtcatg aagtcgtgat caaattccgt aaggaaggca
     3781 cagacggaat taagatctac ggcagaaggg gaagtgagca ggaatttact tttcttgctg
     3841 tcgatacttc ctcaccatat aacgacacga gagagaagat cgaatcgggc aagcccgaac
     3901 agcgggaata ctatgcttac tatttcgaag ctgacagtga aatcggacaa gcgtcggatg
     3961 ttctgaaagt tgtaataccg taataatata attcactcat ccaatccggc aggcgcgaag
     4021 atcttcatga acgcctgccg ggtttaattt taaattgtaa atgatccata tcgcttggga
     4081 tttaaagttt acattataaa gatttccaat ccgaatttaa tttacattga caaataaatt
     4141 gtacctgagg tttgttaaat ttgagtattg agtattgagt ataattatat gatgctggat
     4201 tgtcttgtcc tgaaataggt ttagaaaatg tcagtaacat tagagttgat attatgatta
     4261 aaatttaatt gattagtcgt ggatatttaa aattgaatga cttaaatttt gaacttacct
     4321 ggattaaagc agttaataga tcgaacaaat aagaaa
//
LOCUS       DAORVQ010000244         4345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_15153_length_4345_cov_8.446154, whole genome shotgun sequence.
ACCESSION   DAORVQ010000244 DAORVQ010000000
VERSION     DAORVQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15153_length_4345_cov_8.446154"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..3108
                     /locus_tag="PLX80_13460"
     CDS             160..3108
                     /locus_tag="PLX80_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY21843.1"
                     /translation="MKTSILFFAITVMVLFSLFLNDDTTSSDDLTNYINDKDYIEGEI
                     IVMFKESVNAQDFLNNYSDIGMRVKEVLVKDMNIYLFGYDVARSQPVDALMSVMRNDK
                     VAIAQFNHRFQEREMPPPIIPNDTRFAEQWDKNNTGQSGGTPDADIDAPEAWEVSKGG
                     KTSLGDTVVVAIVDGGQQTNHVDLDTWVNYYEIPGNNIDDDNNGYVDDIFGWNAGTNN
                     GTIPANSHGTHCAGIAGAIGNNGIGVAGVNWTVKTMPVVYGSATEANAIKAYGYILKQ
                     RKIYNQSNGAMGAFVVSTNSSWGIDNAQPSNYPLWCAFYDSLGVAGILSCGAGPNNNV
                     NIDIVGDMPTTCPSQYLIAVTNTTNTDAKNSGAGYGPINMDIGAPGTSILSTLPGNTY
                     GNNTGTSMATPQIAGAIGLMHAAAGSVYTQLGRTRPDSLAKLFKQYILSTVDTLPSLA
                     GITVSNGRLNVNKLVNKVKITNVPVLNNFNLQNPTAGQVITTLPNSTSTVTFTWDTCA
                     TGVEYKWIFGAPLISNRVFSMSSGTNSITFTLGQLDNMLAGIGINQGQTYAGQWTANA
                     YRQLPNVDSLSAQNGPRSVSLARGVPSLAAFNLSSPPTNTTIQTTPTDYSIVSPKWTK
                     SGLGVRYKWMFAYPDFSNAANLKIVVESGNSGYDTSASIRTSQLDSLAASLGAGISDS
                     VSGQWRVYGYSAADSSASAQTYNLTLRRLPVSTITIGAGTSDESYPMNRFYNYFRWQG
                     IYLSSEINTTGQIRKIKFFQNNNVGGITSENVRIFMKTTTDITLPTGTWDTTGMTLVF
                     EGNINSLSDPGWMEIQLTTPFAITSTQNLMIAIGRDFQKYVNIYPRYAYTTSATYLSR
                     RGQSDTQYPTSLTQSFSRANIQMDISLLTGVESNTVSIPGVYSLSQNYPNPFNPATKI
                     SFSIPKQGFVNMKVYDILGKEVMTLVNEIKQPGNYDVNFNGSNFASGVYFYRIESENF
                     VDIKRMMLIK"
     gene            complement(3263..4273)
                     /locus_tag="PLX80_13465"
     CDS             complement(3263..4273)
                     /locus_tag="PLX80_13465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY21844.1"
                     /translation="MLKLVTFSKTEDNKTSNNFTFLFSFVSQFFYLLTHIHNFDIVYI
                     WFADYHSFLAVFFSKFFKKKSYIAVGGYDATYIPEINMGLFTKGSLKKRFRCFCATYS
                     LKNCSMILPVDKSLIENENHYIYFDQPGKPVLKDGIKNFIKEIQTPFHTIRLGYDPEI
                     FKPDSIQKENAIISVGLIENENEYRRKGFDILTEAAKKMPDTKFILIGLNEYYLKYLR
                     SLNLKNTELYGKVSYDELIRHYSRSKVYSQLSMFEGMPSAVCEAMLCGCIPVGSDVNG
                     IPDIIGDCGIVVREKNVDIAVEAFEKALKFDDKASENSRKRIMEMFPIHKREEELLKV
                     VE"
BASE COUNT         1433 a          793 c          782 g         1337 t
ORIGIN      
        1 actattaact attaactatt aactattaac tattaactat taactattaa ctaataacta
       61 ataactatta actattaact attaactatt aactattaac tattaactat taacttatat
      121 ctttataaaa ttaaccaaca ttaaaaaaca ttttaaaaaa tgaaaacatc aatacttttt
      181 tttgcaataa cagtaatggt attattttct ttgtttctga atgatgatac taccagcagt
      241 gacgacctta caaattatat taatgataag gattatatag aaggtgaaat tatagtaatg
      301 ttcaaagagt ctgtcaatgc tcaggatttt ttgaataatt attctgatat cggaatgaga
      361 gtaaaagaag tactcgtaaa ggacatgaac atatatcttt tcggatatga cgtagcaaga
      421 tctcagccgg tagatgcttt gatgtcagtc atgagaaatg acaaagtcgc catcgcacaa
      481 ttcaatcaca gatttcagga aagggaaatg cccccgccta ttattcccaa tgacacaaga
      541 tttgctgagc agtgggataa gaataataca ggtcagagcg gtggaactcc tgatgcagac
      601 attgatgcac ctgaagcgtg ggaagtttcc aaaggcggaa agacttctct tggtgatact
      661 gttgtagttg ctatagtaga cggcggtcag cagactaatc atgtcgacct tgacacatgg
      721 gtaaattatt atgagattcc cgggaataat attgatgatg ataataacgg atatgtagat
      781 gatatttttg gatggaatgc aggcacaaat aacggaacaa ttccggctaa ttctcatggt
      841 acacattgcg caggaattgc aggtgcaata ggaaataacg gcatcggcgt agcaggagta
      901 aactggacag tcaaaacaat gcctgttgta tacgggtctg ctacagaagc caatgctata
      961 aaagcatatg gctatatact aaagcagaga aaaatatata atcagtcaaa cggagcaatg
     1021 ggagcatttg tagtttctac taattcttca tggggaattg acaatgcaca gccatccaat
     1081 tatccattat ggtgtgcatt ttatgattca cttggagtag caggaatatt aagctgcggt
     1141 gcaggtccga ataataatgt gaacattgac attgtcggtg acatgccgac tacctgtccg
     1201 agtcagtatt taattgcagt tacaaatact acgaatactg atgcaaaaaa ttcaggagca
     1261 ggatacggtc caattaatat ggatataggt gctccgggaa caagcatact ttcaactctg
     1321 cctggaaata catacggtaa taatacaggt acttcaatgg caacacctca gatagctgga
     1381 gcaatcgggc tgatgcatgc agctgcaggt tcagtttata ctcagcttgg caggaccaga
     1441 cctgattcac ttgcaaaact ttttaaacaa tatattttat ctacagtaga tactcttcct
     1501 tcactggcag gtattacagt ttccaacgga agactgaatg tgaataaact tgtaaataaa
     1561 gttaagatca caaatgtacc ggttttaaat aatttcaatc ttcagaatcc aactgcaggt
     1621 caggtaatca caacgcttcc aaattctact tcaactgtta cttttacatg ggatacatgt
     1681 gcaactggtg tcgaatataa atggatattt ggagctccgc ttatcagcaa tagagtattt
     1741 tcaatgtctt caggtacaaa ctcaattaca tttactttag gtcagcttga taatatgctt
     1801 gcaggtatcg gaattaatca gggacaaacc tacgcaggtc agtggactgc aaatgcttac
     1861 agacaattac caaatgtaga ttctttatca gctcaaaatg gtccgaggtc tgtttcactt
     1921 gcaagaggag ttccatccct agcggcattt aatttaagtt cacctcctac aaacacaact
     1981 attcaaacca ctcctactga ttattctata gtttctccaa agtggacaaa gtcaggttta
     2041 ggagtaagat ataaatggat gtttgcatat ccggattttt ctaatgctgc caacttaaaa
     2101 atagttgtcg aatcaggaaa cagcggatat gatacttctg caagtatcag aacgtcacag
     2161 cttgattcac ttgcagcaag tctcggtgca ggaataagtg attctgtttc agggcagtgg
     2221 agagtttacg gatacagtgc agcagattct tcagcttcag ctcagactta caatcttact
     2281 ctaagaagat tacctgtctc tacaattacc attggtgcag gtacttctga tgaatcttat
     2341 ccgatgaaca gattttataa ttatttcaga tggcagggaa tttatctcag ttcggaaatc
     2401 aatacaaccg gtcagatcag aaaaattaaa ttttttcaga ataataatgt aggcggtatc
     2461 acaagtgaga acgtcagaat ttttatgaag actactactg acatcacatt gcctacagga
     2521 acttgggata ctacgggaat gactttagtt tttgagggaa atatcaacag tctttcagat
     2581 ccgggctgga tggaaattca gcttacaaca ccatttgcga tcacaagcac tcagaatcta
     2641 atgattgcaa tcggcagaga tttccagaaa tatgtgaata tttatccgag atatgcatat
     2701 actacgtctg cgacttatct ttcaagacgg ggtcaaagtg acacgcagta tccgaccagt
     2761 ctgacacaga gtttcagccg tgcaaatatt cagatggata tctcgctgct cacaggagta
     2821 gaatcaaata ctgtttctat acctggagta tattcacttt cacaaaatta tccaaaccca
     2881 ttcaaccctg caacaaagat aagcttctca attccaaagc agggatttgt aaatatgaaa
     2941 gtatatgaca ttctcggaaa agaagtgatg acacttgtaa acgagataaa gcagccgggt
     3001 aattatgatg taaacttcaa cggcagtaac tttgcaagcg gagtgtactt ctatagaatt
     3061 gaatcagaaa attttgttga tataaagcgg atgatgctta taaaataatt tcaaatttca
     3121 aatacgaaat ttataataaa tactatcccg cagagaataa tttttctttg cgggattttt
     3181 ttattcaatt acgaatttca aatagaatta ctgttgaatc taaaaatttt tctgagttat
     3241 tttttaccgt ataaaaatta acttactcca ccactttcaa aagctcttct tctctcttat
     3301 gaattggaaa catttccata attctctttc gtgaattttc agatgcctta tcatcaaact
     3361 tcagtgcttt ctcaaatgct tcaactgcaa tatctacatt cttttcccgc acaacaattc
     3421 cacagtcacc gattatatca ggaattccat tcacatccga accgacagga atacatccgc
     3481 aaagcattgc ctcgcatacg gcactcggca ttccttcgaa catagaaagc tgcgagtaaa
     3541 cttttgatcg gctgtaatgt ctgataagtt catcgtaact gacctttccg taaagttcgg
     3601 tgttttttaa gttcagactt ctaagatatt tcaaataata ttcatttaat cctatcagaa
     3661 taaatttcgt atccggcatt ttctttgctg cctctgtgag aatgtcaaaa cctttccgtc
     3721 tgtattcatt ttcattttct atcagaccca cacttataat tgcattttct ttttgaatgc
     3781 tgtccggttt aaaaatctcc ggatcatatc cgagacggat tgtatgaaac ggagtctgaa
     3841 tttctttgat gaaatttttt atgccgtcct ttaagacagg ctttcccggc tgatcaaaat
     3901 agatataatg attctcattt tctatcaggc tcttatcaac gggaagtatc atggaacaat
     3961 tctttagaga atatgtcgca cagaaacatc tgaatctttt ctttagacta ccctttgtaa
     4021 aaagccccat attaatttca ggaatgtatg tcgcatcata tcctccgaca gctatataac
     4081 ttttcttttt aaaaaacttt gaaaaaaata cagccaggaa tgaatgataa tctgcaaacc
     4141 agatatagac tatatcaaaa ttgtgaatgt gtgtgagtaa ataaaaaaat tgtgatacga
     4201 aagaaaacag aaatgtaaaa ttatttgatg ttttgttgtc ttctgttttc gaaaaagtta
     4261 cgagttttag caaagcataa ctcagatatt ttattccgac gttaaagaca ttggattgac
     4321 gcacacaaag ctattgagtt aattg
//
LOCUS       DAORVQ010000245         4343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_15171_length_4343_cov_5.498834, whole genome shotgun sequence.
ACCESSION   DAORVQ010000245 DAORVQ010000000
VERSION     DAORVQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15171_length_4343_cov_5.498834"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLX80_13470"
     CDS             complement(<1..344)
                     /locus_tag="PLX80_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21845.1"
                     /translation="MSDLIKETKQVVNMPTSNMKLLLEDILDRGVAPNYILDVGANFG
                     NWTRMAKEVFPQSVIYMIEPLSEMETYLKKITENFPETKYFPCGAGRKNENHVITTWG
                     DNLAEANCVVPE"
     gene            640..1179
                     /locus_tag="PLX80_13475"
     CDS             640..1179
                     /locus_tag="PLX80_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21846.1"
                     /translation="MSPRLDWTDVPTADTYNIIISLDREFTQLVVQILGIQESYYDIA
                     NPYLDFDDIYYWKVRGGLAGFGPWSETFSFRPISVIPPVPVLLSPSNGGINVSRNPTL
                     DWNDVGEIYNYRVQVSRYPNFSPLIFNPGVEHISQYTITNTLPYLPPTRARRSRQLRD
                     SSLRLLLVTLASLTKTNIY"
     gene            complement(1522..2532)
                     /locus_tag="PLX80_13480"
     CDS             complement(1522..2532)
                     /locus_tag="PLX80_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY21847.1"
                     /translation="MQNTKNEIYKHYIALDWAKENFALASIQANSIKYKVEEYPSTIK
                     KLKECLSKFSGKKILTIEETTTAHWLYVELFEHVDKVLICEPYYNSLLKSGPKNDKSD
                     AIKLCTLLRNGSLKEVFHTTDELYRLRKLVSAYDDFVKASVRLKNQRSAMFLSEGKDH
                     KKEKILSGNGINMFVTQKQNLIIEEIESVRNEFVKVFKKLKRENPTVSQLIQVPGIAE
                     ILAVTILAVVIDANRFADKYKYYAYSGLVKYKKVSGGRNYGKKNVRYSRPLKSSYKIA
                     ANAAIGGNNDIREYYEYLIQNNTGTEDARNEIARYISKVSYAIMKYKTDYRPYQWREN
                     MK"
     gene            complement(2857..3576)
                     /locus_tag="PLX80_13485"
     CDS             complement(2857..3576)
                     /locus_tag="PLX80_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21848.1"
                     /translation="MNKKQSNRLRMLIGVSAYLAENTEIVTEIPEMSAAKNNLDGIIS
                     DINYNLNVSNTALFGKTEDKLTAHADVIKYVMALTGGLYAWAVKTGNNEIKSAANVSR
                     SDFGKIRESEFAIVVKDLVERSKEKAKDDSQELADFGVTEELITILENYLENYSSALG
                     KREAYFGKRTGAVRSLKELFSDSINALAVLDKFAVLQSLKHKTFYHEYLVVRRIKNIG
                     EHSAKAQPVTEVSNPTLPRAS"
BASE COUNT         1177 a          820 c          696 g         1650 t
ORIGIN      
        1 ttttcaggaa caacacaatt tgcttcggct agattatctc cccaagtagt tattacatga
       61 ttttcatttt ttcttccggc accacacgga aaatacttag tctccggaaa attctcagtt
      121 atttttttta gataagtttc catttctgaa agaggctcaa tcatatatat gacagattgt
      181 gggaaaactt ctttagccat tctcgtccag ttaccaaagt tagcacctac gtccaaaata
      241 taatttggtg caacccctct atccaaaata tcttccaata atagtttcat atttgatgtt
      301 ggcatattga caacttgttt agtttccttg ataagatcag acataataaa attttatttt
      361 ataatttttt tttcattact taatgcagaa tgtataatgt ttagctgagt ttgtgtcctt
      421 taccatcctt ctaagaacat aaaaataaag aattggaaaa ttatacacaa cataaaaagt
      481 tctaaaacaa attggaaaaa tcaactctgt caaaacgatg taaaaaaata agactataat
      541 cctgtaattt tcatcccgtt atttgttgta aatttaactc aggctcagct tgttccgcca
      601 attttgctct cccccacgga ttcatcagta atatatgttt tgtctcccag gctggattgg
      661 actgatgttc ctactgctga tacatataat attattattt cacttgacag agaatttaca
      721 cagcttgtag ttcaaatact tgggatacag gaaagttatt atgatattgc taatccatat
      781 ttggattttg acgacattta ctattggaag gtgagaggag gacttgccgg atttggtccc
      841 tggtcggaaa catttagttt cagacctatt tcagttattc cacctgttcc ggttctttta
      901 tctccttcta atggtgggat aaatgtttca cgtaatccta ctttagactg gaacgatgtt
      961 ggagaaatat ataactatag agttcaggtc tcccgttatc ctaatttttc accattgatt
     1021 tttaatcctg gagttgagca tatatcacaa tatacaatta cgaatactct gccatatttg
     1081 ccaccaacaa gagcccgtcg aagtcggcag ctgcgggatt caagtctccg tttgttgttg
     1141 gtcacgctcg cttcgctcac aaagaccaac atctattaac aaataaatca agtataaacc
     1201 tcacccctga caaaaaatcc cgttttttgc ctgctgattt atggggctca cttatctagc
     1261 tggagaaagc tgatctcata gaaataatta aagcttactt ttaatctaaa ttttttttcg
     1321 atctttcttt acttaatttc ttttcggttt ttaatattaa acgtctatat gagcctcgag
     1381 ggctattgta aattataatt taaaaccgga agaggttaat cgacaatggt tgactctcta
     1441 tggagtatgt tggtctatga acctttcttc cgaattatag atcgtttttt tagactaacc
     1501 ggctttaata attttgagat tttatttcat attttctcgc cactgatagg gccggtaatc
     1561 tgttttatat ttcattatcg cataggatac ttttgatata tatcttgcta tttcgtttct
     1621 ggcatcttct gtgccagtat tgttttgtat taaatattca taatattctc tgatatcatt
     1681 attacctccg atcgcagcat tggcagctat tttataactg ctcttcaatg gtctgctgta
     1741 tcgaacattc tttttaccgt aatttctacc gccgctaact tttttatatt tcaccagtcc
     1801 gctgtatgca taatatttat atttatccgc aaaacggttt gcatctatca ctactgccag
     1861 tattgttact gctaatattt cagctattcc tggtacttgg atcaattgac ttacagtcgg
     1921 gttttcccgt ttaagttttt taaatacttt tacaaactca ttccggacac tttctatttc
     1981 ctcaattatt aagttttgct tttgagttac aaacatatta attccatttc ctgaaagtat
     2041 tttttctttc ttatgatctt ttccttcgga aagaaacatt gctgatcttt gattcttcaa
     2101 tctgactgag gctttaacaa aatcatcata tgcactgact aattttctta aacgatataa
     2161 ttcatcagtt gtatgaaata cctcctttaa agatccgttt ctcaaaagtg tgcacaactt
     2221 tattgcatca ctcttgtcat ttttaggacc gctttttaat aagctgttat aatacggctc
     2281 acaaattaaa actttatcaa catgttcaaa cagttccaca taaagccagt gagcagtagt
     2341 tgtctcttct atagtcagaa tcttttttcc tgaaaattta ctcaggcatt cttttaactt
     2401 cttaatggtc gatggatatt cttcaacttt atatttaatg ctattagctt gaattgatgc
     2461 taaagcaaaa ttctctttcg cccagtccaa agctatgtag tgcttatata tttcattttt
     2521 tgtattttgc atttgaggct cctttctttt tttagaagtt cattattcca cgaaaataat
     2581 tcgtgctaac tctaaaagaa agtgagcctt tttgttaatt tatcgacggc gaaaatgcga
     2641 agtggatttt attgctgcta agacttacag gagaaggatt caagtttcgg tttgttgttg
     2701 gtgatcaaaa agtttgctta aaatgcgaag taatattcaa ctgaacctca ggcaaacttt
     2761 ttgaaaacac caactacggc gcaaagataa aaagtgaaaa gcaaaaagcc ggagatgaat
     2821 ttccggctta tggtttgtgg tgatatgttt tttattttat gacgcccggg gcagggtcgg
     2881 attgctgacc tctgttacgg gctgagcttt tgctgaatgc tctccgatgt ttttgatcct
     2941 tcttacgacg agatattcat gatagaaggt tttgtgtttc agactctgga gcactgcaaa
     3001 tttatcaagt acagccagcg catttattga atctgaaaaa agttctttca gtgatctgac
     3061 agcaccggtc ctttttccga agtaagcttc tcttttgccg agagcgctgc tgtaattttc
     3121 cagataattc tcaagtatgg tgatgagttc ttccgtgact ccgaagtctg caagttcctg
     3181 tgaatcatct tttgcctttt cttttgatct ttctacaaga tctttaacga cgattgcgaa
     3241 ttctgattct ctgatctttc caaaatcgga tctggataca tttgctgcgc ttttgatctc
     3301 gttgtttccg gtctttacag cccaggcgta aagtccgccg gtgagagcca ttacatattt
     3361 tataacatct gcgtgcgctg tgagcttgtc ttctgttttg ccgaagagag cggtattgct
     3421 gacatttaaa ttataattaa tgtctgaaat gatgccgtcg agattatttt ttgcggcgga
     3481 catttccgga atttcagtta ctatttccgt attttctgcg aggtaagccg aaacacctat
     3541 cagcattctc agcctattac tttgcttctt attcatttta aatttccttt ctgttttgat
     3601 tttaaatttt ttttattttt ttattttttt tatttttttt atttctgttt ctcttttaga
     3661 tctgtttcca attctgctgc atttgtattt tttgaatctt ctgtatttgc tgtatctttt
     3721 gtatctattg tatctcttgt atcttttgta tctcttgtat cttttgtatc ttttgtatct
     3781 cttgtgtaac ctatttgtct ccgttaaccg gagagctttt taactgatca gtgttagtgt
     3841 agcttttgtt taaaattaat tcatttattt taactcaatt tttaaattct aacctctatt
     3901 tcttaattca taattcttaa ttcttatttt cttagtgtct ccctttataa tattatacag
     3961 gtaaattttc caggcgttcc ccaagatccg gaaatgcatt caaatgtttc aatccgggag
     4021 tattttcgca atttaatctc atgcttcaga tcattcaggt atccggcttc ccggttcatc
     4081 cctttataaa ttacacggca caaaatcccg aacggtcccc aaaccaaaaa aataatttca
     4141 aaaactttaa ttccgcttta ttttcattga atatattttt tcccgcaatg aattctaata
     4201 aacataactt atccaccggg aaccggggaa tccggtctaa acggcagaca atccgcattt
     4261 agagacggac aattcgtttt gagagacgga caattcgttt tgagagacag acaattcgtt
     4321 ttgagagaca gacaattcgt ttt
//
LOCUS       DAORVQ010000246         4293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_15481_length_4293_cov_6.678858, whole genome shotgun sequence.
ACCESSION   DAORVQ010000246 DAORVQ010000000
VERSION     DAORVQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15481_length_4293_cov_6.678858"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..873
                     /locus_tag="PLX80_13490"
     CDS             376..873
                     /locus_tag="PLX80_13490"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQY21849.1"
                     /translation="MAKEKFAESSEKGRHFKLMQLAGKWEGTVKTWFEPEKLADESKI
                     TGEFRSILGGRFIMHEYNYELEGKSHDAISIIGFSIPEQKYQIAYLDTFHSGTSIMFS
                     EGEDFNKGFSVLGSYGDTDYDERWGWKTDIDLKDKDNLIITAYNVTPDGEEAKAVEIC
                     YKRTG"
     gene            complement(950..1318)
                     /locus_tag="PLX80_13495"
     CDS             complement(950..1318)
                     /locus_tag="PLX80_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21850.1"
                     /translation="MNTKKILLVTSDKELSGIVKISALTLTKLNCQVEIRESFEFEDT
                     INLSKEENLDMILIDADNKELNILKLIEEIRSYEESSSKKIMTVFSDEIDRDEFFKAG
                     CDSIMTKEEFKRVINNILVM"
     assembly_gap    1524..1623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1884..3563
                     /locus_tag="PLX80_13500"
     CDS             1884..3563
                     /locus_tag="PLX80_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQY21851.1"
                     /translation="MLKLLIDEIEVEFFAGETIIQAALRAGNDIPHFCYHPSLSVSGN
                     CRTCLVELENIPKLVIACSTPATEGMKVHTKSEKVVHAQNAVMEFLLINHPLDCPICD
                     EAGECKLQDYAYQYGVGISRFDESKNHKDKRVKLGPEVMFDQERCISCSRCIRFCEEI
                     AKDPELTFVDRGDHVTIETFPGEELDNPYSMNVIEICPVGALTSEEFRFKSRVWEMSF
                     TDTICPGCARGCNTIMGVRNNEILRIEPRENLSVNDYWLCDWGRLNTIKQVNDEELRF
                     KSPAIKLNEGTTENIGLLDVNWDEAISKSVSHLKNYSGSDIMFIASAHSTLEDNYALK
                     KFAKEIFNSDNVFYIPHTDENFGDEILRKSDMTPNSSGLKMLGIDQYSDSVVDQLSSG
                     KIKMLFILNEDVKGIRGADVFMNNIETTVQLISFKNSSSASANVILPVSTYSEINGTF
                     VNFQNRIQRLRPAVTTLEQERLIGEFSLSRLDKFGAHNDRWTRGTKFNARPGWKIIKL
                     IAKAMGHEFDFDNSEEVFTELAGTIPDMNGYDYDTLGKIGITIGEKPVVEA"
     gene            3600..4106
                     /locus_tag="PLX80_13505"
     CDS             3600..4106
                     /locus_tag="PLX80_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQY21852.1"
                     /translation="MLTKKLQDALNEQINKEFFSEYMYLSMSAYLESIEMEGFANYFN
                     VQAQEEHFHAMKMFNFVHDKGGRVILKPLGEPKANFKSVLKVIEEALEHERFITKSIN
                     ELMDVAIKENDHSVKSFLDWYVDEQVEEEATMGRLIAKLKLINGEGLGLLTIDNELLS
                     RKFSPSEK"
BASE COUNT         1414 a          703 c          787 g         1289 t
ORIGIN      
        1 caaaaaaagt aacaaaaaaa gtcgcccgct ggtgataact tgccggaaat tctttcgtcc
       61 cgggtactcg ggactgcaaa ttagaaaaac attaatgggt tcgtcaaaca atatattttt
      121 gcttaacgcc attgttcaga atttcttatc ggcaatttat cacaggcagg ttattttcag
      181 caaaatttac agtgaaataa aaatgttagg ttagttttta attaaaatta taaactccat
      241 atattaaaat ttttcaaatt atttattcat atcaaaatgt tttggacggc acttgtaatt
      301 ttatcaattt ctattcacaa ctctgtttca aaaaaagatc attattatta cattctcata
      361 aaattaaata taaaaatggc taaagaaaaa tttgcagaat catccgaaaa aggaagacat
      421 ttcaagctta tgcagcttgc aggtaaatgg gaaggaacag tcaagacttg gtttgaaccc
      481 gaaaaacttg ctgacgaatc aaaaatcaca ggggagttca gatcaattct cggaggtaga
      541 tttattatgc atgaatataa ttatgaactc gaaggtaaaa gtcatgatgc aatctccatc
      601 atcggattca gtattcccga acagaaatat cagatcgctt atctggatac ttttcactcg
      661 gggacaagta tcatgttctc tgaaggtgaa gattttaaca aaggattttc agtgctcggc
      721 agctatggtg acactgatta tgatgaaaga tggggatgga aaactgatat cgatttaaaa
      781 gacaaagaca atcttattat tactgcttac aacgtaactc cagacggtga agaggcaaaa
      841 gctgtggaga tatgttataa gagaaccggc tgatccagat tatactgaag ttttcgtttc
      901 cgctgagtca agagctcttc ggactgagcg gttcgctgaa attagaaatt cacatcacaa
      961 gaatattatt gatgaccctc ttaaattctt cctttgtcat aatactgtca catcccgctt
     1021 tgaaaaattc atctctgtcg atctcatctg aaaaaactgt cattatcttt tttgatgatg
     1081 attcctcata ggatcttatt tcctctatga gttttaatat atttaattct ttattatcag
     1141 catcgattaa tatcatgtct aaattttctt cctttgataa atttatagta tcttcaaatt
     1201 caaaagactc tctgatctca acctgacaat ttagtttggt cagagtcaaa gctgatattt
     1261 ttacaatacc cgaaagttct ttgtcggatg ttacaagaag tatttttttt gtgttcatgt
     1321 ttttttgtct caaagactct aagacaaata gttaatagtt aatagttagt agttaataaa
     1381 ttagggtcaa ttgttgaaaa tttaaaaatg cttttctttg agttttcgaa ccttcgaggc
     1441 aacctaaaaa cagcctgaga acttttttaa ctcaatttta aaaatgcttt tcattgagtc
     1501 ttcgagcctt cgagtcaacc ctannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnacagcct gagaactttt ttaactcaat tttaaaaatg cttttcattg agtcttcggg
     1681 ccttcgagtc aaccctaaaa acagcctgag aactttttta acacaatttt aaaaatgctt
     1741 ttcattgagt cttcgagcct tcgaggtaaa cctaaaaact gatgcgaatt aaagaacttc
     1801 aaattgtgaa ataaactgaa ttgaaaaaag ataaaaatag aagtaattta aaacctaaat
     1861 ttttccattc actgaattta aatatgctta aattattaat agacgaaatt gaagttgaat
     1921 ttttcgcagg ggaaaccatt attcaggctg ctctcagagc cggcaatgac attccacatt
     1981 tttgctatca tccgtcactt tctgtctcgg gtaactgccg tacttgtctc gtcgaacttg
     2041 aaaacattcc caagctcgta atcgcctgct ctacgccggc aacagaaggt atgaaagttc
     2101 atactaaatc tgaaaaagtc gtgcatgcgc aaaatgcagt tatggaattt ctgctgatca
     2161 atcaccctct cgactgtcct atctgtgatg aagcgggtga atgtaaactt caggattatg
     2221 catatcagta cggtgtcggt atatcgcgtt ttgacgaaag taaaaatcat aaagataaaa
     2281 gagtaaagct cggacctgaa gtcatgttcg atcaggaaag atgtataagc tgttcgagat
     2341 gtatcagatt ctgtgaagaa attgcaaaag atcccgagct tacatttgtt gacagaggtg
     2401 atcatgtaac gatcgaaaca tttccggggg aagaacttga caatccttat tccatgaatg
     2461 taatcgaaat ctgtcctgtc ggtgcgctga cgtctgaaga attcagattc aaatcacgcg
     2521 tctgggaaat gtcttttact gacacaatat gcccgggctg cgcccgcggc tgcaatacaa
     2581 ttatgggtgt aagaaataat gagatcctcc gaatcgaacc gagagaaaat ctttcggtaa
     2641 atgattactg gctgtgtgac tggggaagac tcaatactat caaacaggtc aatgatgaag
     2701 aacttcgttt caaatcacct gcaataaaac tgaatgaagg cactactgaa aatattggac
     2761 tacttgatgt aaactgggat gaagcaattt ccaaatcagt atcacatctg aaaaattata
     2821 gcggctctga cattatgttt atagcttcag ctcattctac actcgaagat aattatgccc
     2881 taaagaaatt tgcaaaagaa atatttaaca gtgataatgt tttttatata cctcatactg
     2941 atgaaaactt tggtgatgag atattgagga agtcagatat gacgcctaat tccagtggtt
     3001 tgaaaatgct aggtatagat caatatagcg acagcgtagt ggatcagctg agttcgggaa
     3061 agataaaaat gctttttatt ttgaatgaag atgtaaaagg aatcagaggt gcggatgtat
     3121 ttatgaataa tattgagact actgtacaac ttatttcatt taaaaactct tcatcagctt
     3181 ctgcaaatgt aatccttccc gtttctactt attctgagat aaacggaacc ttcgttaatt
     3241 ttcaaaacag aattcagaga ctaaggcctg ctgtgacgac acttgagcag gaaaggctta
     3301 tcggtgaatt ttcactgtcg agactggata aatttggtgc gcataatgac agatggacac
     3361 gcggtacaaa gttcaatgcc agaccgggct ggaagatcat taagctgatt gcaaaagcta
     3421 tgggacatga gtttgatttt gataattcag aagaagtgtt cactgaactc gcaggcacta
     3481 tacctgacat gaacggatat gattatgata cacttgggaa aatcggtata accattggtg
     3541 aaaagccggt ggtcgaagca taaaaatttt ttgttaaaaa ctaaatcaag gagaaaaaaa
     3601 tgttaaccaa aaaacttcag gacgcattaa acgaacagat aaacaaagaa tttttttctg
     3661 aatatatgta cctttctatg tcagcatatc ttgaatctat tgagatggaa gggtttgcaa
     3721 attattttaa tgtacaggca caggaagagc attttcatgc aatgaagatg tttaactttg
     3781 ttcatgacaa aggcggcaga gtaatactga agccactcgg tgagcctaag gcaaatttta
     3841 aatctgtctt aaaagtaata gaagaagctc tcgagcatga aagatttatc acaaaaagta
     3901 tcaacgaact tatggatgta gcgataaagg aaaatgacca ttccgtaaaa agttttcttg
     3961 actggtatgt cgatgaacag gtagaggaag aggcaacgat gggtagatta attgctaaac
     4021 ttaagctgat aaacggagaa ggtcttggac ttctgaccat tgacaatgaa cttctttcaa
     4081 ggaagttttc accttctgaa aaataatttt tttttaaatc accaccggca gaatctatct
     4141 tgcttttgat gactctgccg gagtcaattt tttttaatta aaaaaattag ttttaaaggg
     4201 aaatattatg aagactataa tattaattct attagccatt tgcttttcag gaaaggatat
     4261 ccacgctcaa aatgcatggt atcatcagca aag
//
LOCUS       DAORVQ010000247         4218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_15967_length_4218_cov_5.241893, whole genome shotgun sequence.
ACCESSION   DAORVQ010000247 DAORVQ010000000
VERSION     DAORVQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_4218_cov_5.241893"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLX80_13510"
     CDS             complement(<1..480)
                     /locus_tag="PLX80_13510"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21853.1"
                     /translation="MKFYLFLILKIFIALTNSQSQTNWYWQNPLPQGNDLNHIQFANN
                     STGFAVGNKGTFAKTTNGGENWSYNFQITGFNISSMAVINSNTIFISADKKIMRSTDS
                     GIWWDTVGYSSYFAFSSLFFVDQNTGYSTTNNNFSEFGEVYKTTNGGSNWFHVNINSS
                     "
     gene            complement(493..2709)
                     /locus_tag="PLX80_13515"
     CDS             complement(493..2709)
                     /locus_tag="PLX80_13515"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21854.1"
                     /translation="MKYIKNIIILFIWFFISDISYGQTSWVWQNPLPQGNDLTSVKFF
                     DDNTGWAVGKKGTILKSTDGGINWNIQNSTVYENLNSVFILSEQKVLALGEYGVVVLT
                     TNGGVNWSFSLLAENPSLKKFFFTDEVTGYICGYRADIRKSYIYKTTDGGIYWNLTFT
                     GPESEFFDKIFFWDKNTGITGGDWFAYKTTNAGVNWIQQSYLIRGNELTFKDYNEGMI
                     VGGFSSGYVRKTTNGGVSYYTLFNSNDTVFNSISMVNSFTGYVSGKYGSIRKTTDYGY
                     SWNSLISNVNENLSSIYFSGNNNGNAVGNSGIIINSTNEGMNWSNQRKGMTHTFYSSI
                     FQNENTGWITGTFGIIKTTNGGSNWQNVWDSICYGNLDNLLKFTDDQNGWLLHTPSFQ
                     YFNLYKTSNSGNNWSLIISLNSADFTYYPYTLDFINNNTGFVAGHSVEFIGPSIASYG
                     FVYRTTDGGQTWERSLFDNKFMYDVEFVNASTGFLAGDMDKVLKTTNTGSNWNYLNMN
                     LTGYKYYKICFINEDLGWILGEQRSNGVDPVFNAILKTTNGGSNWENQLPLNSISGLK
                     SLQFIDKNIGWTCGNGGLIYATSNGGQNWIKQDNPANQNLYSINFVDYNTGWVCGSNG
                     TILKTNSGIIPVGIITSNITAPQNFILSQNYPNPFNPITIIRYSIPSDVRGQTSDVKL
                     VVYNNLGKEIITLVNEKQNPGSYAVDFNGEGLPSGVYFYKLEAGEFEETKRMILLK"
     gene            complement(2723..4135)
                     /locus_tag="PLX80_13520"
     CDS             complement(2723..4135)
                     /locus_tag="PLX80_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21855.1"
                     /translation="MKKMYLLISLLVTSMSFAQQGSSILFDSTHFGIRSFHTFDIEGT
                     NQKLVYLQQGTVQNSSHISGGNFLVLNIDRLSWGNAEFLNSLCCAAQNECYPVDYLAV
                     AKTDPNVVLFNGLFECTSPLSSGDYTKISMNGGMTSSELPFKGTDTIQQCRGFDISKT
                     NSQVMYMAHNYFNNVYYEQPRIFKSTDGGLNWFVTDTLQGMKPISPNVGPGFGFLKSM
                     TWNDNVVFTSTYDHLAYSTTGGYDFQVRTDLPPFKMIVFDEGDLWIHAVSYDNKIYCN
                     NGGITNNWLPLSNPFNITCIEIDPEDHTIWYAGSDNSGVWKSVNAGLTFSPYNNSFTP
                     SKKVIGISKDAGSGDTIIVATDKRVYKVWEGLLVNVESVETSLPESFSLSQNFPNPFN
                     PVTVIRYSIPSDVKGMSSNVRLNVFDNIGKQVAALVNEKQSAGSYEVNFKAEGLPSGV
                     YFYKLEAGEFVETKRMILLK"
BASE COUNT         1328 a          822 c          648 g         1420 t
ORIGIN      
        1 tgaagaattg atattaacat gaaaccaatt tgaaccgccg tttgtagttt tataaacttc
       61 accaaactca gagaagttgt tgtttgtcgt tgaatatcct gtattttgat ctacaaagaa
      121 caatgaacta aatgcaaaat atgatgagta tccgacagta tcccaccaga ttcctgaatc
      181 agtagatctc ataatttttt tgtctgcgga aataaaaatt gtattactat ttataactgc
      241 catagatgaa atattaaaac ctgtaatttg aaaattataa gaccaatttt ctcctccatt
      301 tgtagttttt gcaaatgtac ctttatttcc tacagcaaac ccggttgaat tatttgcaaa
      361 ttgtatatgg tttaaatcat ttccctgtgg taatggattt tgccaatacc agttagtttg
      421 ggattgtgag ttagttaaag caataaatat tttaagtatt agaaataaat aaaatttcat
      481 tacaataaat ttttatttta ataaaatcat ccgcttcgtc tcctcaaatt cacccgcctc
      541 aagtttataa aaataaaccc cgcttggtaa accttcgcca ttgaagtcaa ccgcatagct
      601 ccccggattt tgcttttcat taactaaagt aatgatctct ttccccaaat tattataaac
      661 aacaagcttt acgtctgatg tctgacctct gacatctgac ggaattgaat aacgaattat
      721 tgtaattgga ttaaaagggt ttggatagtt ttgtgatagt ataaaattct gtggtgcagt
      781 aatattgctt gtaattattc caaccgggat tatacccgag ttcgttttta agatagtgcc
      841 attgctgccg catacccacc ctgtattgta atccacaaaa ttaattgaat ataaattttg
      901 atttgcaggg ttatcctgct ttatccagtt ttgtccacca tttgaggtgg cataaatcaa
      961 accaccattc ccgcatgtcc agccaatgtt tttatctata aactgtaatg attttaagcc
     1021 ggaaatacta ttcagcggca gttgattttc ccagttcgat ccgccgttag tagtttttaa
     1081 aatagcatta aagacaggat ctacaccgtt acttctttgc tctcccagta tccatcccaa
     1141 atcttcgttg ataaaacata tcttataata tttataccct gtaagattca tgttaaggta
     1201 attccagttt gaaccggtgt tagttgtttt caaaacttta tccatatcac cggctagaaa
     1261 accggttgat gcatttacaa attcaacatc atacatgaat ttattgtcaa acaaacttct
     1321 ttcccaagtt tgccctccat cagttgttct atatacaaac ccataactag caattgatgg
     1381 cccaataaac tcaacgctat gcccggctac aaatcccgta ttattattta taaaatctaa
     1441 agtatatgga tagtaggtaa agtcagccga attaagagaa ataattaaac tccagttgtt
     1501 tcctgagttt gaagttttat ataaattgaa atattggaaa gaaggagtat gcaaaagcca
     1561 accattttga tcgtctgtaa actttaataa attatctaaa tttccatagc agatactatc
     1621 ccagacattc tgccagtttg atccgccatt tgttgttttt attattccaa acgtacctgt
     1681 gatccatcct gtattttcat tttgaaaaat acttgaatag aatgtatgtg tcattccctt
     1741 tcgctgattt gaccagttca ttccttcatt tgtgctgttt ataattatgc ctgaatttcc
     1801 aacagcattc ccattattgt tgcctgaaaa ataaattgag ctaaggttct cattcacatt
     1861 gctgattaat gaattccagg aatagccgta atctgtggtc ttacgaattg atccatactt
     1921 tcctgacaca tatcctgtaa atgaatttac catgcttatt gagttgaaaa cagtatcatt
     1981 gctgttaaac agtgtgtaat aacttactcc tccgttagta gtttttctta cgtagcctga
     2041 agaaaatcca cctactatca ttccttcatt gtaatcttta aatgttaatt cattccctct
     2101 tattaaatat gattgctgta tccagtttac tcctgcatta gtagtcttgt aagcaaacca
     2161 gtcaccaccg gtaattccgg tattcttatc ccaaaaaaat attttatcaa aaaactcact
     2221 ctccggtccg gtaaaagtta aattccagta tatacctcca tcagtagttt tataaatata
     2281 actctttctt atatcagctc tataaccaca gatatatccc gttacttcat ctgtgaaaaa
     2341 aaatttttta agactaggat tttccgccaa cagactgaaa gaccagttaa ctccgccgtt
     2401 tgtcgtcaat actacaacgc catattcacc taatgccaat accttttgtt cagataaaat
     2461 gaatacgctg tttagatttt catacacagt actgttttga atattccagt taattcctcc
     2521 atcggtactt tttaaaattg tacctttttt tccgacagcc catccggtat tatcatcaaa
     2581 aaattttacg gaagtcagat catttccctg cggtaatgga ttttgccaaa cccagcttgt
     2641 ttgtccataa gatatatctg aaatgaaaaa ccagatgaac aaaataataa tattcttgat
     2701 atatttcata acaattttaa ttttatttca ataagatcat tcttttcgtc tcgacaaact
     2761 cacccgcctc cagtttataa aaataaaccc cgcttggtaa accttctgct ttaaagttca
     2821 cttcataact ccccgcactt tgcttttcat taactaaagc tgcaacctgt tttccaatgt
     2881 tatcaaagac gttcagtctc acatttgatg acataccttt gacatctgag ggaattgaat
     2941 atcgaatgac ggtaacagga ttgaaaggat taggaaaatt ctgagacaat gaaaaacttt
     3001 caggaagtga tgtttccacg gattcaacat taacaagcag accttcccaa actttataaa
     3061 ctcttttgtc agtcgcaact attattgtat ctcctgaacc tgcatccttt gaaataccta
     3121 tgactttttt tgaaggcgtg aaagaattgt tataaggtga aaaagttaag ccggcattta
     3181 ctgatttcca gactccgctg ttatcagaac cggcatacca gatcgtgtga tcctcagggt
     3241 ctatttcaat acaggtaata ttaaatggat ttgaaagcgg cagccagtta tttgttattc
     3301 ctccattgtt acaataaatt ttattgtcat aggatactgc gtgtatccac aagtcacctt
     3361 catcaaacac gatcatctta aacggtggca gatcagtacg cacctgaaaa tcatacccgc
     3421 cggtcgtgct gtatgcaaga tgatcatagg tagaagtgaa gacaacatta tcattccagg
     3481 tcatggattt tagaaatcca aatcccggac cgacattagg acttattggt ttcattccct
     3541 gcaatgtatc ggttacaaac cagttcagtc caccatcagt acttttaaaa attcttggct
     3601 gttcataata cacgttgttg aaataattat gagccatata cattacctgt gagtttgttt
     3661 tggaaatgtc aaatcctctg cattgctgga ttgtgtctgt tcctttgaaa ggaagttctg
     3721 atgatgtcat accgccgttc atcgaaattt ttgtatagtc tcccgatgac aaaggcgagg
     3781 tgcattcaaa aagtccgtta aaaagcacta cgttcggatc ggtctttgca acagcaagat
     3841 aatcaactgg ataacattca ttttgagcgg cgcagcaaag agagttcaga aattccgcgt
     3901 tgccccaact aagcctgtca atattaagaa ctaaaaaatt tccgccggat atatgggagc
     3961 tgttttgaac tgtcccctgc tgaagataaa ctaatttttg atttgttcct tcaatatcga
     4021 aggtatggaa agatcttatt ccgaaatgtg tagaatcaaa caaaatactt gatccctgct
     4081 gagcaaaact catggaagta acaagcaagg aaattagtaa gtacattttt ttcataataa
     4141 atgaattaat aattaatgaa aattttaaac tattctgaaa tgcatttacc gaatttacac
     4201 gaattatttt ttaattta
//
LOCUS       DAORVQ010000248         4208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_16049_length_4208_cov_4.195281, whole genome shotgun sequence.
ACCESSION   DAORVQ010000248 DAORVQ010000000
VERSION     DAORVQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16049_length_4208_cov_4.195281"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..981
                     /locus_tag="PLX80_13525"
     CDS             76..981
                     /locus_tag="PLX80_13525"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQY21856.1"
                     /translation="MNFPSHLLGLRNLDSDQFKNIFSSTDLYLKFIKEKKSLKKDLTG
                     KSVLNLFFENSTRTRISFELAEKKVGLDSVNFNSDNSSISKGESLLDTIKNIEAMKFD
                     FIVVRHNTPGIPLFLTKNTSAKILNAGDGINEHPTQGLLDIYTLQKVYGDLKGLNVCI
                     VGNVSHSRVALSNIFGLKTLGANVSVCGPKSFIPVNIEGLGVKVYFDIDKAVKENDIL
                     NILRVQMERDAGSSLTSVKEFHKYFGITSERIKLNKKIKILHPGPMNINVEIDSDVAE
                     SENSLIFDQVTNGLAIKCALFNLMK"
     gene            988..2268
                     /locus_tag="PLX80_13530"
     CDS             988..2268
                     /locus_tag="PLX80_13530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQY21857.1"
                     /translation="MNLYLKNIKIISPEDGIDTVTDMLIVNGHISKIGNVKRIPKDTE
                     EIDMKGKTAIPGIFDMHVHFRYPGQTHKEDLYSGSMSAANGGVTGVLCMPNTDPPIDS
                     ALIVKDIVDKTKELITDVEVSACATEKREGKKLSQLISLSDAGAVAFTDDGSPVSDPE
                     IMRRVLEYSSQLKRPVIQHCEDMRLSDGGVINEGFISTVMGVKGIPEISETSVIARDI
                     ILTDFVKNSHYHIQHISCGKSVELLRNAKQKNINVTGEVCPHHFILTDKECLEYNVNA
                     KMNPPLRTAEDVEQILEGLRDGTIDVICTDHAPHTEYEKNQGFYAAPFGIVGLETLVG
                     LTYTYLVNTGIISFNDFIRKLSINPRKILNLEKVKIAEGEPANLSILNEKAKWKIDKK
                     KFMSKSVNTPFDGFEVQCKPYCVINNDKIHFTKL"
     gene            2265..3047
                     /locus_tag="PLX80_13535"
     CDS             2265..3047
                     /locus_tag="PLX80_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY21858.1"
                     /translation="MTKIISICIPKGGVGKTTTAVNLAASLAVAEKKTLLIDVDPFGA
                     SAIALGFTPDKIKASLSEIFAFTHSIKSAIHRTDLDYLDFVPSSIYNVNAHDKFIQNL
                     ENRLVIKNALRDIEGFYEYIIIDCPPSLKGISTNALVASDSILVPIKSGHFSLEAVDR
                     LIVYFEWLKEVSNPNLKIEGIVLTMYEKDSKVTKISERELKMKYSEYLLNTIIPNTNL
                     LNEATFYGKPLCLYKLNSEGAEAYLNLAVEIMEKNKKAKIEL"
     gene            3047..>4208
                     /locus_tag="PLX80_13540"
     CDS             3047..>4208
                     /locus_tag="PLX80_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQY21859.1"
                     /translation="MNDIEIPKNFNYSESELKWQAFWEKNKIYESHPNPEKEKYSIVI
                     PPPNVTGILHFGHMFNNTIQDIYCRWKRMLGFEVCWVPGMDHAGIATQVMVEKELSKI
                     GKTKYDLGREDFVKLVWEWKEKNGGVILQQLRKLGASVDWSKERFTLDEGLSLNVRKV
                     FVDLYKKGLIYRGNRIVNWDPASQTAVSDDEIFYEERIEKLYYIKYKIENSDDFVIIA
                     TTRPETMFGDTAVAVNPEDKRYNHLKDKHVILPLLNKIIPVIFDEYVDMEFGTGALKI
                     TPAHDVNDFEIGKRHGLDTVVVLNKDSTLNELTGEFSGMEIHKARNVIVKKLAEEGLL
                     DKIEEYKHNVSLSDRTKAVIEPYLSEQWFVSMKKLAEPALEVVKNGQIKFYPE"
BASE COUNT         1507 a          609 c          788 g         1304 t
ORIGIN      
        1 attgcagcag cttctatact tatacttgat tatatattat ggaatttttt aatcggaatg
       61 taactgataa aaagtatgaa tttcccctca catttattag gacttagaaa tttagatagc
      121 gatcaattca agaatatttt ctcttcgaca gatttatatt taaagtttat taaagaaaaa
      181 aaatctctta aaaaagatct cacaggaaag tctgtgttga atttgttttt tgaaaattct
      241 acacgcacca gaatttcgtt tgaattagct gagaaaaaag ttggactgga ttcagttaat
      301 tttaattctg ataattcttc tatcagtaaa ggtgaaagtc ttcttgatac tattaaaaat
      361 attgaagcta tgaaatttga tttcatagta gttagacata atactccggg aatccctttg
      421 tttcttacaa aaaatacatc tgctaaaata ttaaatgcag gtgacggaat taacgaacat
      481 cctactcagg gactattaga tatttataca cttcagaaag tttatggaga tttgaaagga
      541 cttaatgtct gcattgtcgg aaatgtatct cacagcagag tggcactttc gaatattttc
      601 ggactaaaaa ctctcggtgc aaatgtttct gtctgcggac ctaaatcatt catccctgtt
      661 aatatagaag gtttgggagt taaagtatat tttgatattg ataaagcagt taaagaaaat
      721 gatattctga atattttaag agttcaaatg gaaagagatg caggttcatc attaacttct
      781 gttaaagaat ttcacaaata ttttggtatt acttctgaaa gaattaaact taataaaaaa
      841 ataaaaattt tacatccggg accgatgaac attaatgtag aaatagattc tgacgttgca
      901 gaatctgaaa actcactgat ctttgatcag gttacaaacg gacttgctat aaaatgtgcc
      961 ttatttaatt taatgaaata atttgatttg aatttatatc tgaaaaatat taagatcatt
     1021 tctcctgaag acggcattga tacagttaca gatatgctga ttgtaaacgg acatatttca
     1081 aagatcggaa atgtgaaaag gattcctaaa gatactgaag agatcgatat gaaagggaag
     1141 acagcaattc cgggaatttt tgatatgcat gttcatttca gatatccggg tcagacacat
     1201 aaagaagacc tttactcggg aagtatgtct gcagctaacg gcggagttac aggtgtatta
     1261 tgcatgccaa ataccgatcc tcctattgat tcagctctta ttgtaaaaga tatagttgat
     1321 aaaacaaaag aactcatcac agatgtagaa gtaagtgcat gtgcaactga aaagagagaa
     1381 ggcaaaaaat tatctcagct aatttctctg tcggatgcag gtgcagttgc atttacagat
     1441 gacggcagtc ctgtcagtga tcctgaaatt atgagaagag ttcttgaata ttcctcacag
     1501 cttaaacgac cggtcataca gcattgtgaa gacatgagac tatctgatgg aggtgtaatt
     1561 aatgaaggat tcatttcaac tgtgatgggt gtaaaaggaa ttcctgagat cagtgaaaca
     1621 tctgtaattg caagggatat cattctaaca gattttgtaa aaaattcaca ttatcacata
     1681 cagcatattt cttgcggtaa aagtgttgag cttttaagaa atgcaaagca aaagaatata
     1741 aatgtaacag gtgaagtttg tccgcaccat ttcattttaa cagataagga atgtcttgaa
     1801 tataatgtta atgcaaagat gaatccgcca ttgagaactg cggaagatgt tgaacaaatt
     1861 ctcgaaggac ttcgtgacgg aacaattgat gtgatctgta cagaccacgc acctcacact
     1921 gaatatgaaa aaaatcaggg attttatgct gcaccatttg gaattgtagg acttgaaaca
     1981 cttgtcggac ttacctatac ctatcttgta aataccggta ttatttcttt taacgatttt
     2041 ataagaaaat tgtcaattaa tccgaggaaa atacttaatc ttgaaaaagt gaaaattgct
     2101 gaaggggaac ctgccaatct ttcgatactt aatgaaaagg ctaaatggaa gatcgataag
     2161 aaaaaattta tgtctaaatc agtaaatact ccttttgacg ggtttgaagt tcaatgtaaa
     2221 ccttattgcg ttattaataa tgataaaatt catttcacga aattatgaca aaaataattt
     2281 ctatatgtat tccaaaaggc ggagtcggta aaacaacgac agcagtaaat ttagctgctt
     2341 cacttgctgt tgctgaaaaa aaaacattgc tcattgatgt agatccgttc ggtgcttctg
     2401 caattgcact tggctttaca cctgataaaa ttaaagcaag tctttcagag atatttgctt
     2461 ttacacattc gattaaatca gcaattcaca ggactgatct tgattatctg gattttgtac
     2521 cctccagtat ttataatgta aacgctcatg ataaattcat acaaaatctt gaaaacagac
     2581 ttgtcatcaa aaatgcatta agagatatag aaggatttta tgaatacata ataatagatt
     2641 gtccaccttc acttaaggga atttcgacaa atgctttagt tgcatctgat tcaattttag
     2701 taccgatcaa gtcaggtcat ttttctctcg aagcagttga cagactcatt gtatattttg
     2761 aatggctgaa agaagtgtcc aatcctaatc tgaaaataga aggtattgtc cttaccatgt
     2821 atgaaaaaga ttctaaagtt acaaaaattt ctgagaggga attgaaaatg aagtacagcg
     2881 aatatttact aaatactatt ataccaaata caaatttgtt aaacgaagct actttttacg
     2941 gtaagccatt atgtctttat aaactaaatt cagaaggcgc ggaggcatat cttaatcttg
     3001 cagtcgaaat tatggaaaaa aataaaaaag cgaaaataga attataatga atgatataga
     3061 gatccctaaa aattttaatt attcagaatc cgaactaaag tggcaggcat tctgggaaaa
     3121 aaataaaata tatgaatcac atccaaatcc tgaaaaagaa aaatattcta ttgtaattcc
     3181 gcctccgaac gtgacaggaa tattacattt tggccatatg ttcaataata ctattcagga
     3241 catttactgc cggtggaaaa gaatgcttgg atttgaagtt tgctgggtgc ccggtatgga
     3301 tcatgccggt attgctactc aggtaatggt ggagaaggaa ttatctaaga tcggtaaaac
     3361 caaatacgat ctgggcagag aggattttgt aaagctcgtt tgggaatgga aagaaaagaa
     3421 tgggggagtt atcttgcagc agttaaggaa acttggtgct tcagttgact ggtctaaaga
     3481 aagatttacc ttagatgagg gtttgtcgct taatgtacgt aaagtttttg tagatcttta
     3541 taaaaaggga ttaatttaca gaggcaacag gatagttaac tgggatccgg cttcacagac
     3601 tgcggtaagt gatgatgaga tattttatga agaaagaatt gaaaaactct attatattaa
     3661 atataaaatt gagaattctg atgattttgt aataatcgca actacccgtc ccgagacaat
     3721 gttcggagat actgctgtcg cagtaaatcc tgaagataag cgatataacc accttaaaga
     3781 taagcatgtt atattacctc tattaaataa gattatacct gtaatttttg atgaatatgt
     3841 ggatatggaa tttggtacag gtgcattaaa gatcacacct gctcatgatg taaatgattt
     3901 tgaaattgga aaaagacatg gacttgatac tgtagtagtt ttaaataaag attctacatt
     3961 gaatgaatta acaggagaat tttctggaat ggaaattcat aaagcaagaa atgtcattgt
     4021 aaaaaaactt gctgaagaag gattacttga taaaattgaa gaatacaaac ataatgtttc
     4081 gctttctgac aggacaaaag cagtaataga gccttatctt tccgagcaat ggtttgtttc
     4141 aatgaaaaaa cttgctgaac ccgcacttga agttgtaaag aatggtcaga taaagtttta
     4201 tccggaaa
//
LOCUS       DAORVQ010000249         4148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_16444_length_4148_cov_5.670902, whole genome shotgun sequence.
ACCESSION   DAORVQ010000249 DAORVQ010000000
VERSION     DAORVQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16444_length_4148_cov_5.670902"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PLX80_13545"
     CDS             complement(<1..1755)
                     /locus_tag="PLX80_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY21860.1"
                     /translation="MINKLYFIVTLLFISFPLFSQTESENDSLKTDSLKTEKEMEDAE
                     TQEITITGTRIPEKIIDVPFSVFRVDKKELAYSRNVGLKDVLADVPGLFLQSRFGGTD
                     VRVSIRGYGNRSNAGIRGIRILQDGIPESEPDGEASIDVIDYTSLETVEVAKGNFSSL
                     YTNAPGGVINFISDMTFKQNYVKQTNIFGGYGLRQNGIRAGLMTKNYKFYQTYSYRNY
                     NGFREHNNEFINLSNTVFQIYPDSRTTFSLLSNFAKGTIQLPGALTLEEYENAPFSAY
                     PVAVSSDFKRKVSKGKFAARFNKSFGQKIENDIEVTAYVRINDTEYTTNVLYNLNNRY
                     TIGGLIKYDQRSKLFNMKNLFSTGIDYNYVSADISAYNNVAGIKGDELQTQNLEVLDN
                     FGFYFQNQFYIYKDISSITLSGRFDNIKISNDNQLFGFQNSERIFSDFTPRVALNYKL
                     TPTIALYTSYGIGYDSPTAVELQNYPYTSNNGETTLNPDLNPQDTKNFELGIKGNIFT
                     ESILLEKSYFEVTFYNTKIENEIVPFVINDQTFFRNAGNTNRTGVESGFKFEMLEAIE
                     LVVNYSYSDFKYDDYFAVN"
     gene            complement(1894..>4148)
                     /locus_tag="PLX80_13550"
     CDS             complement(1894..>4148)
                     /locus_tag="PLX80_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY21861.1"
                     /translation="IITSSDYITQTLSYEKIQSLGYTILLSQKSYRTDEIVISANKFD
                     ENSKFLPRQIEVLNSEDIAFSNSQNTGDLLQQTGTVFVQKSQMGGGSPVLRGFEANKV
                     LIMIDGVRLNNLIFRGGHLQNVLRIDDNTLNRTEILFGSGSTVYGSDALGGVMSFYTK
                     NPILSLDNNTFKTGTAYYRYSSANTENTGHFDFNFSSKSFGFLGSVTYSNFNSLSMGK
                     SNVKNDAWLRKFNAERINGKDTMIATNNYFLQDPSGYNQFDILGKGLYKPSENIDHTF
                     NFQYSNTTDIPRYDRLNTINPNTGNFTSAQWYYGPESRILGSYKLGLKGKQTFYDNSN
                     ILLAYQNVQESRNNRSFGSSNLTSRSEDVNVYSLNADFSKRINLSNKSSYHDLSYGIE
                     GFYNTVTSTAFRKNINTNVESPADTRYPDGDNNMTTIAGYIMDTWKINEKVYSNIGAR
                     YSYVGLNASFIDTSFYKFAELYPDGISQNNSAFSGNLGFTFLPKDDWKVYINGATGFR
                     APDIDDLAKIFESTPRTGTEYGTVIVPNPDLGPEYTYGGEIGLSNVFSNRIFAQAVGY
                     YTYINDAIVTAPYTYNGSSTIIYDGDTANVQANQNAQTGYIWGTTLSLNADITNFISM
                     TNTVTYTYGRVTTADTTAPLDHIPPLFGKSAFLLNLSKFQGEFSILYNAWKLKKDYST
                     SGEDNFQDATPDGMPNWFTLNVKGQYQLSTNFQMQLEINNLLDQNYRVFASGINAPGI
                     NSVLTLRANY"
BASE COUNT         1310 a          746 c          669 g         1423 t
ORIGIN      
        1 attcacagca aaataatcat catatttgaa atctgaatat gaataattca ccacaagctc
       61 aattgcttca agcatctcaa atttaaatcc gctttccact cctgtcctgt tggtattgcc
      121 ggcatttctg aaaaaggtct gatcatttat cacaaagggt acaatctcat tttcaatttt
      181 tgtattataa aaagtaactt caaaatatga tttttccaga agaatagatt cggtaaatat
      241 atttccttta attcccagtt caaaattctt tgtatcctgt ggattgagat cagggtttag
      301 tgtagtctct ccgttgttag aagtgtaagg ataattttga agctcgactg cagtcggact
      361 gtcatagccg attccataag aagtatataa agcaattgtc ggagtgagtt tataattcaa
      421 tgcaactctt ggagtaaaat cagagaatat tctttcagaa ttctgaaatc cgaacaactg
      481 attatcattt gaaattttta tgttatcaaa tcttcccgat aaagtaattg atgaaatatc
      541 tttataaata taaaattgat tttggaaata aaatccaaag ttgtcaagca cttcaagatt
      601 ctgagtctgc agttcatctc cttttattcc tgcaacatta ttatatgcag agatatcagc
      661 tgaaacataa ttataatcta taccggtaga aaacaggttt ttcatattga ataactttga
      721 tctctgatcg tattttataa gtcctccaat agtatagcgg ttatttagat tgtaaaggac
      781 attagtcgta tattcagtat cattgatcct gacataagca gtaacttcaa tatcattttc
      841 gattttctga ccgaaagatt tattaaatct tgctgcaaat tttccttttg aaacttttct
      901 tttaaaatca ctggagacag caaccggata tgcactgaaa ggagcattct catactcttc
      961 aagagtcaaa gcaccgggta actgaattgt gccttttgca aaattactga gtaatgagaa
     1021 agtagtcctg ctgtcagggt agatctgaaa tactgtattt gaaagattta taaactcgtt
     1081 attatgttct ctgaatccgt tataatttct gtagctgtac gtctgataaa atttataatt
     1141 ctttgtcata agtcctgccc tgattccatt ctgtcttaat ccataaccgc caaatatatt
     1201 ggtttgtttt acgtagtttt gcttgaatgt catatcggaa ataaaattta tcacacctcc
     1261 gggagcattt gtataaagag atgaaaaatt accttttgca acttctacag tttccagaga
     1321 agtataatct atgacgtcga tacttgcttc accgtcaggt tcagattcag gaataccgtc
     1381 ttgcaggatc ctgattcccc ttatccccgc attggaacga ttaccgtagc ctctgataga
     1441 aacgcggaca tctgttcctc cgaatctcga ctgaagaaat aatccgggta cgtcagcaag
     1501 tacatctttt aatccgacat ttctgctgta tgcaagttct tttttatcaa ctctgaaaac
     1561 agagaagggt acatcaatta ttttttcagg gatccttgtt cctgtgattg taatttcctg
     1621 agtctctgca tcttccattt ccttttcagt ttttaatgaa tcagttttca gagaatcatt
     1681 ttcagattca gtttgtgaaa ataaaggaaa tgatataaat aaaagtgtaa caataaagta
     1741 taatttatta atcatgttta gaaaaattta aatgtttaaa attctattcg taaattttaa
     1801 aataagggac ctaattaaag gtccctcgat atatataaat taactaaaga gaaaaagggg
     1861 aattcaatct gttaagttat ataaaaaaca attttaataa ttcgctctta aagttaatac
     1921 agaatttatg cccggtgcat ttatacctga tgcaaagacc ctgtaatttt ggtctaataa
     1981 attattgatc tcaagctgca tctggaaatt agtactcaac tgatactgac cctttacatt
     2041 cagagtaaac cagtttggca ttccgtcagg tgttgcatcc tgaaaattat cctcaccgct
     2101 tgtactgtaa tcttttttga gtttccatgc attatagagt atgctgaatt caccctgaaa
     2161 tttgctaaga tttagtaaga atgcactttt accaaaaagc ggcggaatgt gatcaagagg
     2221 tgcagttgta tctgcagtcg ttactcttcc ataagtatat gtaacagtgt ttgtcattga
     2281 aataaaattt gttatatcag cattcaggct caaagttgta ccccagatat aacctgtctg
     2341 agcattttga tttgcctgaa catttgcagt atcaccatca taaataatcg ttgagctgcc
     2401 attgtaagta taaggtgcag tgacgatagc atcatttata taagtataat aaccaactgc
     2461 ctgtgcaaat attctattgg aaaaaacatt tgataatcca atctcaccgc cgtatgtata
     2521 ttcaggtccc agatcaggat tcggtactat tacagtacca tattctgtac ctgttctcgg
     2581 tgtagattca aatatttttg caagatcatc aatgtcagga gctctgaaac cggttgctcc
     2641 attaatatag accttccaat catctttcgg taaaaaagta aatccaagat ttccactgaa
     2701 tgcactgtta ttctgactga ttccatcagg ataaagctca gcaaatttat aaaaagatgt
     2761 atcgataaat gaagcgttaa gtcctacata gctgtatctc gcaccaatat ttgaatatac
     2821 tttttcattg atcttccagg tgtccattat gtatccggca atagttgtca tattattatc
     2881 accgtcagga tatcttgtgt ctgcaggaga ttcaacattt gtattgatat tttttctgaa
     2941 tgcagtcgat gtaactgtat tataaaaacc ttcaatacca taacttagat catgataact
     3001 gcttttattg cttaaattaa ttctcttact aaaatcagca ttaagcgaat atacatttac
     3061 atcttcagat ctagatgtca gatttgatga accaaaactt ctgttgtttc tgctttcctg
     3121 tacattctga tatgccagca gaatatttga attatcataa aaagtttgtt taccttttag
     3181 tccaagctta taagatccga gtattctgct ctccggacca tagtaccact gcgcagatgt
     3241 gaagtttcct gtatttggat taattgtatt cagtctgtcg tatcgcggaa tatctgtagt
     3301 atttgaatac tgaaaattaa atgtatgatc aatattctct gatggtttat acaatccttt
     3361 cccaagaata tcgaactgat tataacctga aggatcctgc agaaaataat tattcgtagc
     3421 gatcatggta tcttttccgt taattctttc agcattaaat tttctaagcc atgcatcatt
     3481 ctttacatta cttttaccca tgctcagaga attaaaattg gaataagtca cacttccaag
     3541 aaagccaaat gattttgaag aaaaattaaa atcaaagtga cctgtatttt cagtattggc
     3601 agatgagtat ctgtaataag cagtacctgt tttaaaagta ttattgtcca gacttagaat
     3661 gggattcttt gtataaaagc tcataacacc gccgagcgca tcactaccat agacagtaga
     3721 tcctgacccg aataaaattt cagttctgtt caaagtatta tcgtcaattc tcaaaacatt
     3781 ctgcaggtga ccgcctctga atatcagatt gttaagtctg acaccatcga tcatgatcag
     3841 aactttatta gcttcaaatc ctctgagcac cggacttcct ccccccatct gagatttttg
     3901 gacaaataca gttcctgtct gctgcagcag gtctcctgta ttttgagaat ttgagaatgc
     3961 aatatcttca gaattcagca cttcgatctg tctcggcaaa aattttgaat tttcatcaaa
     4021 cttatttgca gagataacta tctcatcagt cctgtaactt ttttgagaca aaagaatagt
     4081 gtagccgagc gactgaattt tttcatagct taaagtctga gtaatataat cagatgaagt
     4141 tatgatta
//
LOCUS       DAORVQ010000250         4135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_16545_length_4135_cov_4.275490, whole genome shotgun sequence.
ACCESSION   DAORVQ010000250 DAORVQ010000000
VERSION     DAORVQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16545_length_4135_cov_4.275490"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            704..1840
                     /locus_tag="PLX80_13555"
     CDS             704..1840
                     /locus_tag="PLX80_13555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY21862.1"
                     /translation="MIKLSEIKTDCRYFKGHIPCSPNKKYGVVCNSCDYYDKTDGRIL
                     IIKLGAAGDVIRTTPLLFPISQQYPNAKIYWLTYFPDLVPKKNRPCADRVFIYNLQNV
                     NYLSELDYELVINLDKDHEAISIMSKINSKRKEGFTMKDGHCYPVNENAVGKFITGLF
                     DSVSISNKKSYMEEIFEICGYKFKGERYILELNKDADLFTNGKYNISKDKDKLNIGLN
                     TGCGARWISRLWKNEYWEKLINLLMNKGYRVILLGGSQEDENNKNLSKITGADYFGYF
                     ELESFITLMDKCDIIVTQVTMGMHIAIALNKKLVLMNNIFNPNEFELYGNGIIASPDK
                     ECKCYFQPECTNKEYQCMDHLTPEKIFSECERLADELKINESVK"
     gene            1907..1980
                     /locus_tag="PLX80_13560"
     tRNA            1907..1980
                     /locus_tag="PLX80_13560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1941..1943,aa:Arg,seq:tct)
     gene            complement(2347..3783)
                     /locus_tag="PLX80_13565"
     CDS             complement(2347..3783)
                     /locus_tag="PLX80_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21863.1"
                     /translation="MIEYLNKFSSEEVLRFEKFVKSPFYNSNKNLLKLTDFLIALYPD
                     INKKNSSKSVISSKVFNEKKINDVKIRKLMSDFNKVIDRFIMQIEFESDNVRNRILLL
                     NSLRKRGMDKRFEYDFNEFSKSQKNLFSKDEDYYNNEVNLLSEYYSHNQSKYKNIKAE
                     ILQEKSDNIDMRFIFEKLHTFHEMFDNEGSRNRDKFFNRTFYEEIKSYIENNLKNVRD
                     EHPNIYIIYLVLKMNETFNDDYLDSLLKFLKANQKKFTNYNLVYYYNYITAYYNKKIN
                     VGHIKYRKDLIKLYMRMMKNDLFLIDNIITDFEYNNVVNIALAEGKYDWIDDFIETYK
                     IYLEKTFAEDIYNLAKAKLLFHLEKYDNIFEYLNKVDIKDPTYYINSKFLLGRVYFEK
                     DEDESIQYILDNLLQYKRNKKILSEEQQKSIQLYLGFMNDLMKISNCPELEKDRKKSL
                     KAIMRKELSNPDGFIPARSWFYEKLEKV"
BASE COUNT         1392 a          657 c          588 g         1498 t
ORIGIN      
        1 attttcataa ttataagttt caaattaatg ataatgatta tttgtatcaa gaacattaat
       61 aaaatgcacc tgttgatagc ctccaggtct cgaagtctca aacgagtatt tttctctttc
      121 gtaccaatac tccacagact gtccgaaatc aaattatttc gtttgcctca aagtctcaaa
      181 gaatcaaagg attttatttt ttcactcatt ttgttattcc cgcgtattcc acattttgtc
      241 attccttttt cgaaaaacta tctgttaatt attttttttc ttcgataaac ttctcaataa
      301 attatttgct actctaatat attttgtgcc tgtattacct cccgccatta tgtaaacaca
      361 atatgtattc ataccattct atctgaactt tcagattgga ttcccgctac gagcacgcag
      421 gaatgacatg aggggaaagt tccgctcgat cccaaaatcc cagcctgttc gaatacactg
      481 atttttccac taatgcacaa agacattaaa ttaaaaacaa taataataaa ttactttgag
      541 tcttagagtc ttagagtctt agagtcttag aggcatgatt aaattccaag ccattgataa
      601 attaattctt attgaccggt aatttttttt acagatttat tttactattt taatcaataa
      661 aataattcaa tagatctgag aatgtttatt taaattttac aaaatgataa aactttccga
      721 aataaaaacc gactgcagat atttcaaagg tcatatacca tgctctccaa ataaaaaata
      781 tggtgtcgtt tgtaatagct gtgattatta tgataagaca gatggtagaa ttttgattat
      841 aaaactgggt gccgcaggcg atgtcatcag aaccactcct ctgctatttc ccataagtca
      901 gcaatatcca aatgcaaaaa tttactggct tacttatttt cccgatcttg tccctaaaaa
      961 aaataggccc tgtgccgaca gagtctttat ctacaacctt cagaacgtaa actatctttc
     1021 tgaattggat tatgaacttg ttataaatct tgacaaagat catgaagcga tcagcattat
     1081 gagtaaaatt aattccaaaa ggaaagaagg ttttactatg aaggacgggc attgttatcc
     1141 tgttaatgaa aatgcagttg ggaaattcat tacaggatta tttgattctg tttcgataag
     1201 taataagaaa agttatatgg aggaaatatt tgaaatttgc ggatacaaat tcaaaggaga
     1261 acgatacata cttgaattaa acaaagatgc cgatcttttt acaaatggaa aatataatat
     1321 cagtaaagat aaagataaat taaatattgg attaaataca ggctgcggtg cacgatggat
     1381 ttcccgatta tggaaaaatg aatactggga aaagctgatc aatcttttga tgaataaggg
     1441 atacagggta attcttctcg gcggtagtca ggaagatgag aacaataaaa atctgagcaa
     1501 gataaccggt gcagattatt ttggttattt cgaactggaa agttttataa ccctgatgga
     1561 taaatgcgac atcattgtta cgcaggtaac tatgggaatg cacatagcca ttgctctaaa
     1621 taaaaaactt gtactgatga acaatatttt caatcccaat gaatttgaat tatatggcaa
     1681 tggaataata gcaagtcccg ataaagaatg caaatgttac tttcagcctg aatgtacaaa
     1741 caaggaatat caatgtatgg atcatctgac ccctgaaaag atctttagtg aatgtgagag
     1801 actggcggat gaattaaaga tcaatgagtc tgtaaaatag ctgtatattt aatttgaatg
     1861 catttgttaa attagttatc ttacacgttt cttaaaggaa actaatgcgc ccgtagctca
     1921 atcggataga gcaacagcct tctaagctgt aggttactgg ttcgattcca gtcgggcgta
     1981 ctttggatgc gggattttaa gaaagcggtt ttaaccgctt tctttgtttt gagacgggaa
     2041 cttgatgtga agatggtagg ataatattaa agtcagaaga acaggtgata tcagatttat
     2101 cttttcatta tttattttgt tgaaatgcct gcgatattat cttttctatg gttacagttt
     2161 tttgataatc cgtttagtat tctattacat cctaacctaa tcacaatata ctaagtaatt
     2221 ttttactcaa tttttaaaac cttcctaagc ccaggagcaa taaatgttta ataattcttt
     2281 taggttctga ctatgcaagt gaatgattta acactataaa cataatttac ttcatttaat
     2341 tatgtattat accttctcaa gcttttcata aaaccagctc ctggccggaa taaacccgtc
     2401 cggatttgac aactcttttc tcataattgc tttcagactt ttttttctat ccttttcgag
     2461 ttccggacaa tttgatattt tcataagatc attcataaat ccaagataaa gctggatgct
     2521 tttttgctgt tcttccgaaa gaattttctt gtttcgttta tactgtagaa gattatcaag
     2581 tatatactgt atactttcat cttcatcctt ttcaaaataa accctcccca gtaaaaactt
     2641 tgaatttata taataagttg gatcctttat atctacttta ttcagatatt caaaaatatt
     2701 atcatacttt tccaaatgga aaagtagttt tgcctttgca agattataaa tatcctcagc
     2761 aaaagtcttt tccaaatata ttttgtaagt ttcaataaaa tcatctatcc agtcatattt
     2821 tccttctgcc agagcaatat tcactacgtt attatattca aaatccgtta taatattgtc
     2881 tatcagaaac agatcatttt tcatcatcct catatatagt tttatcagat cctttctgta
     2941 tttgatatgc ccgacattta ttttcttgtt ataatatgca gtaatgtaat tgtaataata
     3001 gactaaatta taatttgtaa atttcttttg atttgcttta agaaatttca ataatgaatc
     3061 aagatagtcg tcattaaaag tttcattcat cttgagtact aaataaataa tataaatatt
     3121 gggatgctca tctctgacat tcttaagatt attttcaatg taactcttta tttcttcgta
     3181 gaatgttctg ttgaaaaact tatccctgtt acgggaacct tcattgtcaa acatctcatg
     3241 aaatgtatga agcttttcaa agataaatcg catgtcaatg ttatccgatt tctcctgaag
     3301 aatctcagcc tttatatttt tatatttact ttgattatga ctataatact cactcaataa
     3361 atttacttca ttgttataat aatcttcatc ttttgaaaat aaatttttct gacttttact
     3421 aaactcatta aaatcatatt caaacctctt gtccattccc ctttttctca atgagtttaa
     3481 cagtagaatt ctattcctca cattatcaga ttcaaattcg atctgcatta taaatctatc
     3541 tatcacctta ttaaaatctg acattaattt tctgatcttt acatcattta tttttttttc
     3601 attaaaaacc ttggatgaga tcacagattt tgaagagttt tttttattaa tatccggata
     3661 aagtgcaatc agaaagtcag ttaattttaa cagattttta tttgaattat agaacggtga
     3721 tttcacgaat ttttcaaatc gcaagacttc ttcggaagag aacttgttta aatattcgat
     3781 catatttttt taattttttt taaaaaacaa cttttttttt cagaaaaaaa tttttagtca
     3841 attctcacaa accctttatt aacagtactt tggaaggggt caaaaaccgt tttttcacaa
     3901 atggtctttt ttaccatttg actcgtgtct gtttttttta taattttgtc aagcttctta
     3961 attgaagatg tagtttaaaa aaagttctaa gaaattaaaa gattttaaat ttaccggtaa
     4021 aattttaaaa tcgaaaaaag aacatttaat gaactactaa aagcaacaga gttaatttaa
     4081 aaatcctgag gaagaaacaa aatgaaaaat ttagaaacaa tttttaacaa gattt
//
LOCUS       DAORVQ010000251         4132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_16567_length_4132_cov_4.739760, whole genome shotgun sequence.
ACCESSION   DAORVQ010000251 DAORVQ010000000
VERSION     DAORVQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16567_length_4132_cov_4.739760"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..786
                     /locus_tag="PLX80_13570"
     CDS             58..786
                     /locus_tag="PLX80_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21864.1"
                     /translation="MQNKLIDLKGIEKMYVMGTEVLYALKGVDLEIKKNDYIAIMGPS
                     GSGKSTLMNIIGCLDTPSAGEYYLNGKNVSEMDDDELAEIRNLEIGFVFQTFNLLARS
                     DALHNVELPLVYKGLSKAERIEKAEEALYNVGLEDRMTHKPNELSGGQRQRVAIARAL
                     VNDPSIILADEPTGNLDTKTGNEIMELLEELNQKGNTIIIVTHEEEIALHAHRVVKIR
                     DGNVESDELSEKGRAVLERKAIEI"
     gene            805..2031
                     /locus_tag="PLX80_13575"
     CDS             805..2031
                     /locus_tag="PLX80_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21865.1"
                     /translation="MNLFLEIKENIIISYKAITANKLRSFLATLGIVIGITSVTLMQT
                     VIEGINRAFEKSISAVGADVLYVQKFEWFGKEDWSFYRNRKDITMTQFEYLERNAQTA
                     ASLCPSTGSQKTLKYKDLVLENVFVLGTTAEYQVTFGANMEEGRFFTQKESNGGYPVC
                     VIGMDIKNAFFDNVDPIGKTIKIGTYSFRIIGVVEKQGSLLGLISLDNRVIIPIERFF
                     RLFGNKRSLSINIKAGDINDLDETKEEVISIMRKARKVSYGERDDFGVNQQEAFKSTY
                     ESLTSLIKIIGSLITSLSLIVGSVGIANIMFVSVKERTKEIGIRKAIGAKKRTILLQF
                     LIESVTICLLGGLIGLMISVPISLIIDAYVLPTAMPLWVIILGLVVSVIFGVLAGFFP
                     AYNAAKMNAVDSLRYE"
     gene            2040..3272
                     /locus_tag="PLX80_13580"
     CDS             2040..3272
                     /locus_tag="PLX80_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY21866.1"
                     /translation="MQIIELIRIALNTLKNNKIRAMLTLSGIVIGVFSIIAIMTLLNA
                     LQAGIESGLNSLGSNTYQVQKFPAMQFGDARSNKKFRNRKDITYDEGLRLQEKATLYK
                     FISIEDYVYQKSIKTNKDQTNPNFYIGGVVPDYLECNNKTIQYGRFFTQTDLDRNAKI
                     AAIGTDVVDKLFPNVDPIGKDIIIDNSQYKVVGVFKKEGEGFGESNDNFVLLPITSMQ
                     QAYGKNNRSMNIAIQAPSKELFNESIENIVTVLRSIRGDKPGEPDSFEIFSNESLIGQ
                     VNNFTKYFKYGAGFISFIALLAAGIGIMNIMLVSVTERTKEIGIRKAIGAKSKSILIQ
                     FLVEAVILCELGGIIGIFLGVLTGNLLGIYLNSPVVIPYDWVVIGLLVCSAVGIGFGV
                     YPAYKAAKLNPIDALRYE"
     gene            complement(3283..>4132)
                     /locus_tag="PLX80_13585"
     CDS             complement(3283..>4132)
                     /locus_tag="PLX80_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY21867.1"
                     /translation="TLIGINNIYTDENTAFIKFSDSFKKAGLKVPEVFGVSHDSQIYI
                     EEDLGDMTLYKYSQTAPHGNTSDLFKKALCDLQDFQIKGIDCIDFSYCYQSKEFSEET
                     IYSDFDKFEDYYLRKIKNTEIDEELFNYVKGFLSELNSNSDKEYFLYRDFQTRNIMVK
                     ENELYYIDYQSGRKGPPHYDAASFLYSGSINITEEERNFFLRHYIDSFSNKINLNKEK
                     FKYEFYFFALMRLIQILGSYGYTYEKKKDEMMLKKIPKAIENIRGLKNYITEPLLLKF
                     IERISA"
BASE COUNT         1443 a          611 c          778 g         1300 t
ORIGIN      
        1 agaagaagat taaattaaaa gaataaatta gaagaataat tttaaagttc atatataatg
       61 cagaataagt taattgatct aaaaggtata gagaaaatgt acgtcatggg tacggaagtt
      121 ttatatgctc ttaaaggtgt tgatctggaa ataaaaaaaa atgattacat tgcgattatg
      181 ggaccttcag gttcaggtaa atcaacactc atgaatataa tcggatgtct tgatactcca
      241 tctgccggtg aatattatct taatggtaaa aatgtaagtg aaatggatga tgatgaactt
      301 gctgaaataa gaaatctcga gatcggtttt gtgtttcaga ctttcaatct tcttgcaaga
      361 agtgatgcgc tccataatgt agagctgcct ttggtttata aaggattgtc gaaagctgaa
      421 aggatcgaga aagccgagga agcattatat aatgtagggc ttgaagacag aatgactcat
      481 aagccaaacg aactttctgg aggacaaaga caaagagttg caatagcaag agctttggta
      541 aatgacccat ctataatact tgcagatgaa ccgacaggaa atcttgatac aaaaacaggc
      601 aatgagatta tggaattact tgaagagctt aatcaaaaag gaaacactat aattatagta
      661 acacatgaag aagaaattgc actgcatgct cacagagtag ttaagatcag ggatggaaac
      721 gttgagagtg atgagctaag tgaaaagggc agggccgtat tggaaagaaa ggcaattgaa
      781 atttgaaaaa gtcattaata tatcatgaat ttatttcttg agataaaaga aaatataata
      841 atttcctaca aggcgattac agccaataag ctgagatcat ttcttgctac gcttggtata
      901 gtgattggaa tcacttccgt tacacttatg cagacagtta tagaaggaat aaacagggca
      961 tttgaaaaaa gcatttccgc agtaggagct gatgtattgt atgtgcagaa attcgaatgg
     1021 tttggaaaag aagactggtc attttacaga aacagaaaag atattactat gacacagttt
     1081 gaatatcttg aaagaaatgc tcagactgca gcatcacttt gcccttcgac aggctcacaa
     1141 aagactttga aatataaaga ccttgtactg gaaaatgttt ttgtacttgg tactacagca
     1201 gaatatcagg ttacattcgg agctaatatg gaagagggca gattttttac acagaaagaa
     1261 tcaaacggcg gttatcctgt ctgcgttata gggatggaca taaagaatgc attttttgat
     1321 aatgtagacc cgatcggcaa gacaatcaag attggtactt attcttttcg gatcataggt
     1381 gttgtcgaaa agcagggaag tcttcttgga ttaatcagct tagataacag agtaataatt
     1441 ccgattgaga gatttttcag attgtttggt aataaaagaa gtttatcaat aaatatcaaa
     1501 gcaggtgata taaatgatct ggatgaaacc aaggaagaag tgatatccat aatgcgtaaa
     1561 gcccggaagg tatcttatgg tgagagagat gattttggag tcaatcagca ggaagctttt
     1621 aaatcaactt atgaaagtct aacttctctt attaaaatta tcggatcact catcacgtct
     1681 ttatcactga tagttggttc agtcggtatc gcaaacatta tgttcgtttc agtaaaagag
     1741 cggactaaag agattggaat cagaaaagct atcggtgcaa aaaagagaac cattctcctt
     1801 cagtttttaa tagaatcggt cactatttgt ctgctcggag gattgatagg gctaatgata
     1861 tctgtaccga tcagtcttat tatagatgca tatgtactac ccacggcaat gcctctttgg
     1921 gtaataatac ttggattagt agtatcggtt atttttggag tactcgcagg atttttccct
     1981 gcatacaatg ctgccaaaat gaatgcagta gattcattaa gatatgaata aataaaatta
     2041 tgcagattat agaattaata cggatcgctc ttaatactct taaaaataat aagataagag
     2101 caatgcttac attgtcaggt atagtcattg gtgttttttc tataattgcg atcatgactt
     2161 tactcaatgc acttcaggca ggcattgaaa gtggactgaa ttcactggga agtaatactt
     2221 atcaggtaca gaaatttccc gccatgcagt ttggcgatgc aagatccaat aaaaaattca
     2281 gaaatagaaa ggatattaca tatgatgaag gactgagact tcaggaaaaa gcaactctgt
     2341 ataaatttat ttctatagaa gattatgtat atcaaaaatc aattaagacg aataaagatc
     2401 agacaaatcc aaatttttat ataggaggag tcgtaccgga ttatctggaa tgtaataata
     2461 aaacaataca atacggaaga ttttttacac agaccgatct tgatagaaat gcaaaaattg
     2521 ctgcaattgg aacagatgta gttgataaac tttttccgaa tgtcgatccg ataggcaagg
     2581 atattataat cgataacagc cagtataaag tagttggtgt ttttaaaaaa gaaggtgaag
     2641 gttttggaga aagtaatgat aattttgtgc tgctgccaat cacatctatg cagcaggcat
     2701 atggaaaaaa taacaggtca atgaatattg ccattcaagc accaagtaaa gaattattta
     2761 atgagtctat tgaaaacata gttactgttc tcagatcaat aagaggtgat aaaccgggtg
     2821 agccggatag ttttgaaata ttttcaaatg aatctctgat aggtcaggtt aataatttta
     2881 caaaatattt caaatacggc gcgggattta tttctttcat tgcattactc gcggcaggta
     2941 tcggaattat gaatatcatg ctggtatctg taactgaaag aacaaaagag atcggaatca
     3001 gaaaagctat cggtgcaaaa agtaaaagca tacttattca gtttcttgta gaagcagtaa
     3061 tactttgtga acttggaggc attatcggaa ttttccttgg agtgttaaca ggtaatttgc
     3121 tcggtattta tctgaacagt ccggtagtaa ttccttatga ctgggttgtg atcggattat
     3181 tagtttgttc tgcagttgga attggtttcg gtgtttatcc tgcttacaaa gcagctaaac
     3241 taaatccaat cgacgcttta agatatgaat aaccaaacta tattatgctg aaattctttc
     3301 aatgaatttc agcaacaacg gttctgtgat ataatttttc aaacctctga tattttcaat
     3361 cgctttcgga attttcttta acatcatttc atcttttttc ttttcataag tatatccgta
     3421 actccctaat atttgaatca atctcatcaa agcaaaaaaa taaaattcat atttgaattt
     3481 ttccttattt aaattgattt tattgctaaa tgaatctata tagtgtctca gaaaaaaatt
     3541 tctttcttct tcagtaatat ttatactgcc tgaataaaga aatgaagccg catcataatg
     3601 cggcggacct tttcttcctg actgataatc tatataataa agctcattct cttttaccat
     3661 tatatttctt gtctgaaaat ctctgtaaag aaaatattct ttatctgaat tgctgtttaa
     3721 ctctgataaa aatcccttta cataattaaa aagctcttca tcaatttctg tattttttat
     3781 ttttctcaga taataatctt caaatttatc aaaatcagaa taaattgttt cctctgaaaa
     3841 ttcttttgac tgataacaat aactaaaatc aatacaatct attcccttta tctgaaaatc
     3901 ctgtaagtcg catagagctt ttttaaaaag atctgaagta ttcccgtgcg gagctgtctg
     3961 agaatatttg tataatgtca tatcaccaag atcttcttct atatagatct gactgtcatg
     4021 agatacacca aacacttccg gtacttttaa tccggctttt ttaaatgaat cagagaattt
     4081 tataaaggca gtattttcgt cagtataaat attgttgatt cctatcagag tc
//
LOCUS       DAORVQ010000252         4095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_16860_length_4095_cov_6.174257, whole genome shotgun sequence.
ACCESSION   DAORVQ010000252 DAORVQ010000000
VERSION     DAORVQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16860_length_4095_cov_6.174257"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLX80_13590"
     CDS             <1..789
                     /locus_tag="PLX80_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQY21868.1"
                     /translation="NIGEDEFDNITKILSEYPNIRTYGLNKLKHPTRKVNLYYNLYNS
                     DKSARFEKSTEPTYTVNYNDVESLLNMADAVLVNMISGVDITIETLKNIRKNFKGFMH
                     IDIHNIVMETKEDGSRNHVPVDNWYEWCTNTDTVQMNQFEIKVLSREKLTEYKIAEEI
                     LFNNIHEVKGVIVTRGVEGVTGFTKKMKEYGTEKFRDLDHIEIAAIENPNFKDSTGCG
                     DVFASAFTLDYSKNGNFDKSIHFANRLASYSSSLEGISELKELK"
     gene            844..1737
                     /locus_tag="PLX80_13595"
     CDS             844..1737
                     /locus_tag="PLX80_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY21869.1"
                     /translation="MKIFITGSNGLLGQAVTSIFTRETDHELILSSVEENSYLDYNHT
                     YCELDITSKEDVKKLIGYYEPDIIINCAAFTNVDKCETERELSWKINVDGVKNLIIAA
                     KKYDSKIIHFSTDYVFDGKNGPYTEVSKPNPVSFYGREKLASENALITSDINFAIIRT
                     LVLYGIGNKVKPNFALWMIEELRNNRPVNIVTDQISNMTMIDDLAYGAMKIAEKDVTG
                     IYNISGPDILSRYDFALKLCEVFKYNPALVIPITTDSLKQPAPRPLNSGLIILKAQSE
                     LGFNPMDSLEGLRLLKVQLGY"
     gene            1774..3048
                     /gene="serS"
                     /locus_tag="PLX80_13600"
     CDS             1774..3048
                     /gene="serS"
                     /locus_tag="PLX80_13600"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQY21870.1"
                     /translation="MLDIKMIRENPDDVKNKLDLRNEDPAVIEKIFEMDKSRLELIHK
                     TETLKEERNKATDEIALMKRNKENTDEKISEMKKVSENIKMSDEELKKYDADIDILMR
                     YIPNLPHDSVPHGKSEKDNVEIKLSGKKKKFDFEVKDHIQLGKDHDILDFERGTKISG
                     SGFALYKGKGARLERALINFMLDEQGKNGYKEVMTPVIVTRESMEAAEKVPKFEDDMY
                     YMEKDDMFAIPTAEVSILNIHRDEILKSEDLPIKYCGFTNCFRREAGSYGKDTKGFLR
                     VHQFNKVELITFCKPEDSYIEVEKMLSHATGILDKLGLHYRVIELCTADIGFAATKCY
                     DIEVWSYAENKWLETSSVSNCTDFQSRRAQIRYKKMLENNKTKTEFVHILNGSGLATS
                     RLMVALLEANQTMDGNIEIPDVLKEYSGFDKI"
     gene            complement(3247..3765)
                     /locus_tag="PLX80_13605"
     CDS             complement(3247..3765)
                     /locus_tag="PLX80_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQY21871.1"
                     /translation="MNFDLETVLFFSLSVLAVASAILMITRKNPINSALYLILNFFTV
                     SGLFLLLRAQFIAVIQVLVYMGAIMVLFLFVIMLLNLQDKSKLRESYTYKKLTSILLA
                     LMLFGILGYTVFFGFEGKFDKMADNAELNGTAETLGAELFTNFSFPFELASFLLLAAI
                     VGAIVLAKKKFD"
BASE COUNT         1497 a          631 c          783 g         1184 t
ORIGIN      
        1 aatatcggtg aggatgaatt tgacaacatt acaaaaattt taagtgaata tccaaacata
       61 agaacttacg gtttaaataa attaaagcat cctacaagga aagtaaattt atattataat
      121 ctgtacaact ctgacaaaag tgcccggttt gaaaaatcaa cagagccgac atatactgtt
      181 aattacaatg atgtggaaag tctgctgaat atggctgatg ctgtacttgt caatatgatc
      241 tcaggagtgg atattacgat tgaaactctt aaaaacatca gaaaaaattt caaaggcttt
      301 atgcatattg acattcataa tattgtgatg gaaacaaaag aggacggcag cagaaatcat
      361 gtaccggtcg ataactggta tgagtggtgt acaaatactg atactgttca gatgaatcag
      421 tttgaaataa aagtactcag cagggaaaaa cttactgaat acaaaatagc ggaggagata
      481 ctttttaata atatacatga agttaaaggg gtgatcgtta caagaggagt cgaaggagtt
      541 acaggtttta caaaaaaaat gaaagaatac ggcactgaaa agttcaggga tcttgatcat
      601 attgagatcg ctgctataga gaatccgaat ttcaaagact ctacaggttg tggagatgta
      661 tttgcttctg ctttcacgtt agattattca aagaacggaa actttgacaa aagtattcat
      721 tttgcaaaca gacttgcctc atacagttcc tcactggaag ggatctcaga acttaaagaa
      781 cttaagtaaa ttttttttga gatcattaac aattctaact gaaaaaatat ttattaataa
      841 caaatgaaaa ttttcataac aggatcaaac ggtttactcg ggcaggcagt gacttcaatt
      901 ttcacaaggg agacagatca cgaacttatc ctttcctctg ttgaagaaaa ttcctattta
      961 gattacaatc atacttattg cgaacttgat attacttcca aagaagatgt aaaaaaactt
     1021 atcggatatt atgaacctga tattataatt aactgtgctg cttttaccaa tgttgataaa
     1081 tgtgaaactg aaagagaatt gtcatggaag atcaacgtcg acggagtaaa aaatctgatc
     1141 attgctgcaa aaaaatatga ttcaaagata attcattttt cgactgacta tgtttttgac
     1201 ggtaagaacg gtccttatac agaagtctca aagccaaacc cagtatcatt ttacggaagg
     1261 gaaaaacttg caagtgaaaa tgctctaatt accagtgata ttaattttgc gataatcagg
     1321 actcttgtgc tttatggaat tggtaataaa gtaaaaccaa attttgcgtt atggatgatc
     1381 gaagaattga gaaataacag accggtgaat attgtgactg atcagataag taatatgaca
     1441 atgattgacg accttgcata cggagctatg aagattgctg agaaagatgt tacaggtatt
     1501 tataatattt caggacctga cattctttca agatatgatt ttgctttaaa gctttgtgag
     1561 gtttttaaat ataatccggc acttgtaata ccaatcacaa ctgatagttt gaaacagcct
     1621 gctccaagac ctttgaattc aggactgata attctcaaag cgcagtcaga acttggattc
     1681 aatccgatgg attctctcga aggtctcaga ttgctgaagg ttcagcttgg atattagtcc
     1741 gtgtattaaa ttaaataaaa aattaaattt tatatgttag atataaaaat gatcagggaa
     1801 aatcctgatg atgtaaaaaa taaattagat cttcgcaatg aagatccggc tgttattgag
     1861 aaaatttttg agatggataa aagtcgtctg gagctgatcc ataaaactga aactctcaaa
     1921 gaagaaagaa ataaagctac cgatgaaatt gctctgatga aaaggaataa agaaaatacc
     1981 gatgagaaaa tttctgaaat gaaaaaagta tcagagaata ttaaaatgtc agatgaagag
     2041 ctgaagaaat acgatgccga cattgacatt ctgatgagat atattccgaa tcttccgcat
     2101 gacagtgtgc cgcatggtaa gtctgaaaag gataatgtag aaataaaact ctcgggtaaa
     2161 aaaaagaaat ttgattttga agtgaaagat cacattcagc ttggaaagga tcacgatatt
     2221 cttgattttg aaagaggtac aaaaatttcg ggcagcggtt ttgctttgta taaaggaaaa
     2281 ggcgcaagac ttgaacgtgc gctgataaat tttatgctcg acgaacaggg gaagaacggc
     2341 tacaaagaag ttatgacacc ggttattgtc acacgtgaat ctatggaagc cgccgagaaa
     2401 gttccaaagt ttgaagatga tatgtactat atggaaaaag atgatatgtt tgcaattcca
     2461 actgctgaag tttccatatt gaatattcac agagatgaaa ttttaaaaag cgaagatctg
     2521 ccgataaaat actgcggatt cactaactgt ttcagacgtg aagcgggaag ttacggaaaa
     2581 gatacaaaag gttttttacg agtgcatcag ttcaataagg ttgaacttat cacattctgc
     2641 aaacccgaag attcatatat agaagtggaa aaaatgcttt ctcacgcaac aggaattctg
     2701 gacaagcttg gacttcatta cagggtaatt gagctttgca cggctgacat tggatttgca
     2761 gcgacgaaat gttatgacat tgaagtatgg tcttatgctg agaataaatg gcttgagact
     2821 tcttcagtca gcaactgtac agattttcag tcgagaagag cgcagataag atataaaaaa
     2881 atgcttgaga ataataaaac taaaactgaa tttgtacata ttctgaacgg ttccggtctt
     2941 gctacatcga gacttatggt agcacttctt gaagcgaatc agactatgga cggaaatatt
     3001 gaaattccgg atgtattaaa agagtactcg ggatttgata agatttaact atactattcc
     3061 aaatctttaa gccttataat aatattacaa ttgcctctaa cgctctaaaa ctctaagacc
     3121 ctaagtctca aagcaaaaaa acattcataa tatttacctt tgagtcttcg ggacttagag
     3181 gtaaaataaa taattatgta ttcggattgt atgtcagtct ggattactta ataagtgtaa
     3241 aaggatttaa tcgaatttct tttttgcgag tacaatcgcg ccgacaatgg cagccagaag
     3301 taagaaagat gcgagttcaa aaggaaatga aaagtttgta aaaagttcag cacccagagt
     3361 ttcagcagta ccattcaatt cagcattatc agccatctta tcgaacttcc cttcaaaccc
     3421 aaaaaacaca gtatatccga ggattccaaa aagcattaat gccaaaagaa ttgaagttaa
     3481 tttcttataa gtataagatt ccctgagctt acttttgtcc tgaagattca gaagcataat
     3541 tacaaaaaga aaaagcacca tgatcgcgcc catgtaaaca agtacctgta tgacggcaat
     3601 gaactgcgct cggagcagca gaaaaagacc tgatacagta aagaaattca gtatgagata
     3661 taatgcgctg ttaatgggat tttttcttgt gatcattagg attgcagatg caacagctaa
     3721 aacactcagc gagaaaaata aaacagtttc taaatcgaaa ttcatatatt ggattaattc
     3781 aagacatgca aatataagat ttatatttta tttttgtaat tctaaatttg aatccaaaca
     3841 tatttatccg acctttataa aaacaatatt atgaatttgc gtacccgaat ttattcggga
     3901 gaagcaatcc gaattttata catgtctata attcagaaga atactgtttc aaatataaaa
     3961 tgatcaaatg cccgatcaaa gcacccggga atgacgctta ttcggagatt tcttagtcac
     4021 attactcaat aaaaaatcat ttttcagctt ttctccccat cctttctttt taaaggagtg
     4081 ggcgggggag gttga
//
LOCUS       DAORVQ010000253         4071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_17054_length_4071_cov_5.957171, whole genome shotgun sequence.
ACCESSION   DAORVQ010000253 DAORVQ010000000
VERSION     DAORVQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17054_length_4071_cov_5.957171"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLX80_13610"
     CDS             complement(<1..791)
                     /locus_tag="PLX80_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21872.1"
                     /translation="MGNTLPLFLLFQKLDYRDKDNSGFKKLAGIVIAYLFSNTMLSFS
                     FSLVYDERSFVIMSLTSNLFLLTMIVINDFNNLFLATGSIDILRSLPIKIGQVFSAKF
                     LSAVVYLLIFIIAASVPQIIFFYSISESTALSVSFFFTNILFCYLSIGSVTLIYCLIL
                     IYLKKYSTVFLNLFQFLFFIFVFYSTSKGSRPSSFDSQMFQKQDLLSDGFIKYLPQSF
                     FSGSVYEPLIFLILLAVCTVVFLMLYKFISTGYNILLENALSLKR"
     gene            complement(796..1854)
                     /gene="mltG"
                     /locus_tag="PLX80_13615"
     CDS             complement(796..1854)
                     /gene="mltG"
                     /locus_tag="PLX80_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQY21873.1"
                     /translation="MEFSISKIKRYYIYILLLNIILISFSVYQIFFSKYFWDGSAEKK
                     FSVTQGSSLNSVIDDLEKNDIISDRLLFKIAVMLTLNDGNIISKNYILQNGMSNIELI
                     RILTDNTVQLAKVIIPEGYTLKKIAILAEKKFMHSEKKFLLEASNDSLINNLGLKGRI
                     KNLEGFLFPDTYEVSLQITEKEFVEILFDRFREKVLSNDEIESAIENHRDTLLSIITL
                     ASIIEGETNIAEEKPIISGVYHNRLKKNMRLEADPTVQYVIPGGPKSRLLFEDLKFKS
                     PYNTYQNKGLPPGPINNPGLKAILAALNPEDNKFIFFVATGEGGHNFSETYDQHLNAI
                     KEYKKKLRENKAKKNSGK"
     gene            complement(1921..3954)
                     /locus_tag="PLX80_13620"
     CDS             complement(1921..3954)
                     /locus_tag="PLX80_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21874.1"
                     /translation="MKKIKIPVKKKRTNKEDYGKYSFEEPFKPGKVLKIVFYAIVILL
                     PVLLAFIYLRFAVNSNKYMSFPLDDPWIHLTFGKNLIEYFSFSFFKGEMVTAGSTSPL
                     YTLIVAIGFLIVSNEMILSYALGVGFFVLASLAFYRLSSFEFDKENLFALLCTLIFII
                     DKWMNFISLSGMETTMFIFLLLACAYFYRIRSAIPFAVFLGLIIWTRPDGVAFIAAVV
                     IDYLLVKHYSKDQLNLKLFSSKEIKTIGIIFGVIIAAYFGMNYFLSGTILPNTYNAKL
                     TYYSPEFRSRWDFLSLEVWNFFKEGSFYPVSVGGSYYLIMIGFIFSFGKLIYDIYKKN
                     YNQNTLYIAFALIMVFIYWYKLPYAHRFGRYMMPLIPFFILVGTIGFRDIARIINKFS
                     NNPLFSRSIFYILIGITYFNGIKNYDEVRVAYAVECRYIHDRQVNAAYWFQKYSKEGD
                     VIATHDIGAIGFYSGRKVVDVAGLVTPELISKINDKDYVQYMTEYLKEHNVTYLAFLR
                     EWYRVANQTPLYTTPDNISPEIMDIYKFNPDETHVLSREANELVMYGLKLVNQKAAQQ
                     LIHFMNRLLVLEPNYAYAYYLRAYGYSILKDDLNYEKDLNSALSIMPDFKDANLYYGI
                     YLSTKGRFEESKVLLDKVITMEPLNKIAKDNLVIVNDSLNARITQEGSFNQFK"
BASE COUNT         1437 a          665 c          645 g         1324 t
ORIGIN      
        1 tttcttttaa gagataaagc attttctaaa agaatgttat atccggtaga aataaattta
       61 tacagcatta aaaacacaac tgtacatacc gccagtaata ttaaaaatat taaaggttca
      121 taaactgatc ctgaaaaaaa tgactgagga agatatttaa taaacccgtc tgaaaggaga
      181 tcctgtttct gaaacatctg agaatcgaag gaagatggtc tgctgccttt tgaagtggaa
      241 tagaatacga aaataaaaaa caggaactgg aataagttta aaaaaacagt agagtatttt
      301 tttaaataaa tcagtatcag acaatagatc agtgtaacgg atccaataga taaatagcaa
      361 aataaaatat ttgtaaagaa gaaagaaaca gaaagtgctg tgctttcaga aatggaataa
      421 aaaaaaatta tctgaggtac agatgctgcg ataataaata tcaaaagata aacaactgcg
      481 gataaaaact tagcggaaaa cacctgtccg atcttaatgg gaagcgatct tagaatatct
      541 atgctgccgg ttgcaagaaa cagattgttg aaatcattaa tgacgatcat tgtcagcagg
      601 aagaggtttg aagtgagaga cattatcaca aaacttctct catcatacac aagactaaag
      661 ctgaaagaga gcatggtatt ggaaaaaaga taagctataa caatgcctgc aagtttttta
      721 aaacctgaat tatccttatc tctgtaatca agtttttgaa acaataaaaa aagaggtaat
      781 gtatttccca tcatattatt tccctgaatt ttttttagcc ttattttccc ttagtttttt
      841 cttgtactct tttattgcat tcagatgctg atcataagtt tcagaaaaat tatgaccgcc
      901 ttcgcctgtt gcaacaaaga aaatgaactt gttgtcttca ggatttaatg cagcgagtat
      961 agctttcaaa cccggattat ttattggacc gggcggaagt cctttattct gataagtatt
     1021 atatggagat ttgaatttca gatcttcgaa aagaagtctt gactttggac cgccgggaat
     1081 gacatactga acagtcgggt cggcttcaag acgcatattt tttttcagac ggttgtgata
     1141 aactcctgat attattggtt tttcttctgc aatgtttgtc tcaccttcga ttatagatgc
     1201 aagtgtgatt atagaaagta atgtgtctct atgattttct attgcagact ctatttcatc
     1261 attgcttaaa actttttcac ggaacctgtc aaaaaggatt tctacaaatt ctttttcagt
     1321 gatctgcaga gagacttcat aagtatccgg aaataaaaat ccttcaagat ttttgatcct
     1381 gccttttaat cccagattat ttatcagaga atcatttgat gcttccagta aaaatttttt
     1441 ctcagaatgc ataaattttt tctcagccag aattgcaatt tttttcaaag tataaccttc
     1501 gggaataata acctttgcca actgaacagt attgtctgtc aatattctga taagttcaat
     1561 attgctcata ccgttttgaa gaatgtagtt ctttgagata atgttaccgt cgttaagagt
     1621 aagcattact gcaattttga acagcagtct gtcggaaatg atatcatttt tttcaagatc
     1681 atcaataaca ctgttaagac tcgagccctg tgtaacagaa aatttctttt ctgcagagcc
     1741 gtcccagaaa tatttagaaa aaaagatctg ataaacggaa aaagatatca aaattatatt
     1801 cagtaagaga atataaatgt aatatctttt aattttagat attgaaaatt ccaattaaat
     1861 gatttggatt attttgatat ttaagtgcgt tgcctgtcct tagtaatatc aagatctata
     1921 ttacttgaac tgattaaaac ttccctcctg agtaatgcgc gcatttaagg aatcattcac
     1981 gattaccaga ttatcttttg caattttatt taatggttcc attgtaatta ctttatcaag
     2041 aagtactttt gattcttcaa atcgaccttt ggtgctaaga tatattccat aatacaaatt
     2101 agcatctttg aaatccggca ttattgaaag agctgaattt aaatcttttt cataatttaa
     2161 atcatctttc aaaatagaat atccataagc tctcagataa taagcatatg catagtttgg
     2221 ttctaatact aagagacggt tcatgaaatg aatcaactgt tgcgcagctt tttgattaac
     2281 taatttcagt ccatacatga caagttcatt tgcttcacgt gaaagaacat gtgtctcatc
     2341 cggattgaat ttatatatat ccattatttc aggtgaaata ttatcaggtg tagtatataa
     2401 aggggtctga ttagcaactc tataccattc tcttaaaaat gcaagataag taacgttatg
     2461 ctctttgaga tactctgtca tgtattgaac ataatctttg tcattaattt ttgaaattaa
     2521 ttcaggtgta acgagtcctg ctacgtcaac gaccttcctt ccactataaa aaccaattgc
     2581 tccaatatca tgagttgcaa taacatctcc ttctttggag tatttctgga accagtaagc
     2641 tgcattaact tgtctgtcat ggatgtatcg gcattctact gcataagcta ctctgacttc
     2701 atcatagttc tttattccat taaaataagt aataccgatc agtatgtaaa atatacttct
     2761 tgaaaataat ggattatttg aaaacttgtt tattatcctt gctatatctc tgaatccaat
     2821 agttccaaca agtataaaga aaggaattaa cggcatcata tatcttccaa acctgtgagc
     2881 ataaggaagt ttataccaat agataaaaac cattatcaga gcgaatgcta tgtataatgt
     2941 gttttgatta taattctttt tgtaaatgtc ataaattaat ttcccaaaag aaaaaataaa
     3001 tccgatcata atcagatagt aagatcctcc tacagaaaca ggatagaaag aaccttcttt
     3061 gaaaaaattc cagacctcca agcttaaaaa atcccaccgg cttctgaatt caggtgagta
     3121 ataagttagt ttggcattgt atgtatttgg aagtatagta cctgaaagaa aataattcat
     3181 cccgaaatat gcagcgataa taacaccgaa aattattcct atagtcttaa tttctttaga
     3241 cgaaaataat tttaaattta actgatcttt ggaatagtgt tttactaata aataatcaat
     3301 tactacagca gcaataaaag ctactccgtc gggtcgggtc catattatga gtccgaggaa
     3361 aactgcaaac ggaatcgcac tccttatccg gtaaaaatat gcgcatgcaa gaagtaaaaa
     3421 aatgaacatg gtagtttcca ttccggataa cgaaatgaaa ttcatccatt tatctataat
     3481 aaaaattaat gtacagagaa gggcaaataa attttcctta tcgaattcaa atgagcttaa
     3541 tctgtaaaat gcaagtgaag ccagcacgaa aaatccaaca ccaagtgcat aactcagtat
     3601 catttcattc gaaacaatca aaaatcctat agcgactatt aaggtatata gcggggatgt
     3661 agagccggca gtgaccatct cccctttaaa aaaagaaaag ctgaaatatt ctataagatt
     3721 ctttccgaat gtaagatgga tccacgggtc atctaacggg aagctcatat acttatttga
     3781 atttactgca aatctcagat aaataaatgc gagtaatact ggcagcagaa taactattgc
     3841 ataaaaaact atttttaata ctttaccggg cttgaagggt tcttcaaaac tgtattttcc
     3901 atagtcttcc ttattagtcc ttttcttctt tacaggaatt tttatcttct tcatatgaaa
     3961 caaaataatt aattgaatat gtaaattcaa tttttaaatt gctgtggtgt ttgtgaataa
     4021 attacacgac acaaaatccc gaactttccc caaaccaaaa aaataatttc a
//
LOCUS       DAORVQ010000254         4054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_17176_length_4054_cov_4.889972, whole genome shotgun sequence.
ACCESSION   DAORVQ010000254 DAORVQ010000000
VERSION     DAORVQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17176_length_4054_cov_4.889972"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLX80_13625"
     CDS             <1..511
                     /locus_tag="PLX80_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY21875.1"
                     /translation="KLNKIEPEKIYETILQAHLFCGFPAAIESLKIFNEHFSDYKPKI
                     SKYNTSLFSIIGIRNCKLIYNNNFKKLISNITELSPDLKEWMLTDGYGKVMGRKGLNL
                     SEREFINVSVLCTRYYENQLHSHIKGCINLGCDPLDIKNILSELTQTAGKQNVIKALK
                     LLKSIIKSN"
     gene            703..1005
                     /gene="nuoK"
                     /locus_tag="PLX80_13630"
     CDS             703..1005
                     /gene="nuoK"
                     /locus_tag="PLX80_13630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQY21876.1"
                     /translation="MIEINYYLLVSAILFSIGVIGVLVRRNAIIIFMCIELMLNAINL
                     TLVAFSSFLGDINGQIFVFIVMTVAAAEAAVGLAIIISLFRNKESVNIDEINIMKW"
     gene            1030..2943
                     /gene="nuoL"
                     /locus_tag="PLX80_13635"
     CDS             1030..2943
                     /gene="nuoL"
                     /locus_tag="PLX80_13635"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQY21877.1"
                     /translation="MTDIFYLVTILPFIGFLINGLLGSKIKNEKVVGTISTLAVFIPF
                     IIAVVQFSELLSLAPESRSILITYYEWIAAGTFSVNVAYLVDPLSMCMVLVITGIGTL
                     IHIYSIGYMHGDKAFPKFFAYLNLFIFAMLNLVLADNLLLIFLGWEGVGLCSYFLIGF
                     WYEHKFTGDAAKKAFIVNRIGDFGFMLAMFFIFTNFNTLEISKFLEGIVGFQIGDTLL
                     LTIALLLLLGACGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVTAGVYLLARTSLLY
                     AMSPLASQIVFFVGLFTAIFASTIALKQYDIKKVLAYSTVSQLGFMFIALGTGAYWVA
                     IFHLITHAFFKGLLFLGSGSVIHGMHHEQDMRFMGGLRSKMKITYVTFFIGSLAIAGI
                     PPLSGFFSKDIIIFETYLNFGIPLVILSLAAAAMTSYYMFRMVGMTFNGKPRYDEKHL
                     HPHESPSTMTVPLIILAFLSIFGGLIGLPGLYHMPNLLKGWLDPVFEKSVLVLNTLHP
                     HAEHALTPELIIIAVSVIISVFAIYMALKKYSNMEKFEESTGFGKVLENKYYVDEAYD
                     KIIVNPIEKTSEKFLWNIFDVKIIDGAVNGIARYISSISFDWRRIQTGVIQDYAGVSV
                     AGIVLILLYLLIF"
     gene            complement(3013..3801)
                     /locus_tag="PLX80_13640"
     CDS             complement(3013..3801)
                     /locus_tag="PLX80_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY21878.1"
                     /translation="MTQIKISSCSVILIVLFSASIIINGCGKEEKAPEVKDTSENSEK
                     KTEQKETKSQSSPGKEFFYMKSSSNNIACADCHGDGTNSDRPLTKYFSDIKGADKRTS
                     TYHGKIKGEEVKNTAGGATICWESYMKMKTPLTPEQIKMLNEYYTSVGTSDSPTEINY
                     ETIALPTRDKTKLKEVQKEVLKLKGDPVKGETTFKEACAVCHGETATVKKVPSILEEF
                     EGNTKSVTYNVRLGDGSMPFFNMNVLSDQDIADVSEYVLKINGQ"
     gene            complement(3850..>4054)
                     /locus_tag="PLX80_13645"
     CDS             complement(3850..>4054)
                     /locus_tag="PLX80_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21879.1"
                     /translation="FKSVHGDFNDRLESNVENLSINDEEGSFYTNLSDPDNGFQLVNG
                     KKKNIDLWSINVGIVIFLGKSKK"
BASE COUNT         1237 a          621 c          641 g         1555 t
ORIGIN      
        1 aaaattaaat aaaatagaac ctgaaaagat ctatgaaact attttacagg cacatttatt
       61 ctgcggattc ccggcggcta ttgaatcttt aaaaattttc aatgaacact ttagtgacta
      121 taagccaaaa atttcaaaat ataataccag tttatttagt ataataggaa ttcgcaattg
      181 taaattaatt tataataata actttaaaaa gttaatatct aatattactg agttaagtcc
      241 ggatctaaaa gaatggatgc ttacagacgg atatggaaaa gtaatgggta gaaaaggtct
      301 taatctatca gaacgagagt ttataaacgt ttctgtcctt tgtacaagat attacgaaaa
      361 tcaattacat tctcatataa aaggatgtat aaatcttggt tgtgatcctt tagatattaa
      421 aaatatttta tctgaattaa cccaaacagc cggaaaacag aatgtgatta aagctttaaa
      481 gctattaaaa tctataatca aaagtaatta acaatcttaa taattgttat ataatatata
      541 ttatgtaaag tcggatagtt attaaactgt tctaaacaat tttaatgtta atgtcttctt
      601 tgtttactat acttcccttt taaaaattca gctaaaatat ttcttctaaa agtgtaataa
      661 agtatgtttt attttaggtt tataatattt aattttgcac ttatgataga aattaattat
      721 tacttactgg tcagtgcaat tttattcagc attggagtta ttggagtttt agtcagaaga
      781 aatgcaatta taatctttat gtgtattgag cttatgctga atgccattaa tctgactctt
      841 gtagcatttt catcattttt aggagatatt aacggacaaa tatttgtgtt tattgttatg
      901 acagttgcag ctgctgaagc tgctgtagga cttgcaataa taatatcatt attccgtaat
      961 aaagaatccg taaatatcga tgaaataaat attatgaaat ggtaaaatcc ataaatttaa
     1021 cagacttaaa tgactgatat attctactta gttacgatcc tgccatttat cggttttcta
     1081 ataaacggac ttttaggtag taaaataaag aatgaaaaag tagttggtac tatttctact
     1141 ttagctgttt ttattccttt tattatcgct gtagttcaat tttctgaatt attatccctt
     1201 gccccagaat ccagaagtat tctgatcaca tactatgaat ggattgctgc tggtactttc
     1261 tctgttaatg tagcttatct tgttgatccc ctttcaatgt gtatggttct tgttatcact
     1321 ggtattggaa cactgataca tatttattct ataggatata tgcatggtga taaagctttt
     1381 ccgaaatttt ttgcatatct gaatctgttc atttttgcaa tgctgaattt agtattagca
     1441 gataatcttc tgcttatatt tttaggatgg gaaggtgtcg gactttgttc ttatttcctt
     1501 attggatttt ggtatgaaca taagtttaca ggagatgctg ctaagaaagc ttttatcgta
     1561 aaccgtatcg gagattttgg ttttatgtta gcaatgtttt tcatttttac aaactttaat
     1621 actctcgaga tatctaaatt tcttgaaggt atagtcggtt ttcaaatcgg tgatacttta
     1681 cttcttacaa tagccttact actattactc ggtgcatgcg gtaaatctgc acagataccg
     1741 ctttttgtct ggctgcctga tgctatggcc ggacctacac ctgtatctgc tcttattcac
     1801 gctgctacga tggttacagc aggtgtttat ttacttgcga gaacttcatt gctttatgct
     1861 atgtcacctc ttgcttctca gatagtattc tttgtcggat tatttactgc aatatttgct
     1921 tcaacaattg cattaaagca atatgatatt aaaaaagtat tagcgtattc aactgtttcg
     1981 caattaggct ttatgtttat tgctttagga accggtgctt attgggtggc aatatttcac
     2041 cttataacac atgctttttt caaaggtctg ctattcttag gaagcggttc tgtaattcac
     2101 ggtatgcatc atgaacagga tatgagattt atgggtggac tgagatcgaa aatgaaaatt
     2161 acatatgtaa ctttctttat tggatctctt gcaattgcag gtattcctcc cctttctgga
     2221 ttctttagta aggacattat tatatttgaa acttatctta attttggaat tccattagta
     2281 attttatctc tggctgctgc agcaatgaca tcatattata tgttcagaat ggtaggaatg
     2341 acttttaatg gtaaacctcg ttatgatgaa aagcatcttc accctcatga atctccttca
     2401 actatgactg tacctctgat catacttgca ttcttatcaa tattcggtgg tttgattggt
     2461 cttcccggac tttatcatat gccaaatctt ctaaagggtt ggctcgatcc tgtttttgaa
     2521 aaatctgttc ttgttttaaa tactttacat cctcatgctg agcacgcact tactcctgag
     2581 cttatcataa ttgcagtttc agttataatc tcagtgtttg caatttatat ggcattgaaa
     2641 aaatattcaa atatggaaaa gttcgaagag agtactggtt ttggaaaagt tcttgagaat
     2701 aaatattatg ttgatgaggc ttatgataaa ataattgtca atccaattga aaagacttct
     2761 gaaaaattct tatggaatat ttttgatgta aagataattg acggagcagt aaatggaatt
     2821 gccaggtata tttcaagtat aagttttgac tggagaagaa ttcaaactgg agttattcag
     2881 gattatgcag gcgtttctgt agctggtata gttttgatac ttttatattt attgattttc
     2941 tgatctgttg aactttagat gattttatta aaagggataa tattttttat tatccctttt
     3001 ttatttttaa aattactgac cattgatctt taaaacatat tcagaaacat cagctatatc
     3061 ctgatccgat aatacattca tattaaagaa aggcattgag ccgtcaccaa gtcttacatt
     3121 ataagtaact gactttgtat ttccttcaaa ctcttccaat attgaaggga ctttttttac
     3181 tgttgccgtt tcaccgtgac aaactgcgca tgcttctttg aaagttgtct caccttttac
     3241 aggatcacct tttaatttta aaacttcttt ttgtacttct tttaatttcg tcttatccct
     3301 tgtaggcaaa gcaattgttt cataattaat ttcagttgga gaatctgatg ttccgacaga
     3361 tgtataatat tcgttcagca tttttatctg ctctggtgtc aatggagttt tcattttcat
     3421 ataagattcc cagcaaattg tagctccgcc tgcagtgttt tttacttctt ctcctttgat
     3481 ctttccatga taagtcgaag tccttttatc agcacctttt atatctgaaa aatattttgt
     3541 caatggtctg tctgaatttg taccatcacc atgacagtca gcacatgcaa tattgttaga
     3601 tgatgatttc atataaaaaa attctttacc gggagaactt tgggatttag tctctttttg
     3661 ttcggtcttt ttttcggaat tttctgaagt gtcttttact tccggggctt tttcttcttt
     3721 gccgcatcca ttaattataa ttgatgctga gaacaaaaca attaaaataa ctgaacagga
     3781 actgatttta atctgagtca taagttagtt taaatttaaa tttaaataaa agcttaacaa
     3841 atataataat tattttttgg atttacctaa aaatattaca attccaacat taatactcca
     3901 tagatcaata ttttttttct ttccatttac taactgaaat ccattgtcag ggtcagagag
     3961 attagtataa aaactcccct cttcatcatt aattgacaga ttttcaacgt tactttcaag
     4021 tctgtcattg aaatctccat gtacactttt aaaa
//
LOCUS       DAORVQ010000255         4039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_17280_length_4039_cov_4.617972, whole genome shotgun sequence.
ACCESSION   DAORVQ010000255 DAORVQ010000000
VERSION     DAORVQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17280_length_4039_cov_4.617972"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..665
                     /locus_tag="PLX80_13650"
     CDS             138..665
                     /locus_tag="PLX80_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HQY21880.1"
                     /translation="MKTFVKKYNSAVLSLLVMFIIVSCSSKSQKAEAVLESRSGSSVT
                     GKVTFTKEGNVVKIIADIKGLTPGKHGFHIHEKGDCSAPDATSAGGHFNPRSMQHSGH
                     QTTERHTGDMGNITADESGNAHLEISDSIMSFDGEESIIGKSVIVHEKEDDLKTQPTG
                     DAGGRVACGVIVIVK"
     gene            993..1232
                     /locus_tag="PLX80_13655"
     CDS             993..1232
                     /locus_tag="PLX80_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21881.1"
                     /translation="MMDYCIHFFALLYFTGEESAEASFSRSDETVRTIDKDGFVPALL
                     SAEITAKLSLVIRGLYIMNSQKKSAEANPVQKETI"
     assembly_gap    1257..1356
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1411..1539)
                     /locus_tag="PLX80_13660"
     CDS             complement(1411..1539)
                     /locus_tag="PLX80_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21882.1"
                     /translation="MVSECRAVNQPFIVSPFTPPIEASYQAATEIMFLIFSPARLL"
     gene            1654..>4039
                     /locus_tag="PLX80_13665"
     CDS             1654..>4039
                     /locus_tag="PLX80_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HQY21883.1"
                     /translation="MKFELKDYELISNMREFYQLNESDQIITNEEPIRAELYNSAQME
                     RFGTTLAGTHKVSTIPSKDYLLKRLSDNEIKLQEVRKLVTDSIKKKLEITSTGEWLVD
                     NFYLIEENIHIAKKHFPKNYSEGLPHLVDEIYSGLPRIYDIALQNISHCDGIIDIEIL
                     SRYIKAYQKDTKLKLGELWALPIMLRLALIENLRRVAVRVAVSRLDRNLADFWSKKMI
                     ETAENSPKDLILVIADMARTNPPMVSGFVSEFIRKLQGHKANMNLVHNWVENRLSEIG
                     LSISEVVYAENQKQAALQISMSNIIGSLRLLDDTDWRNFVEELSIVEQTLREDKIYGL
                     MDFSTRDRYRHVVEQIAKKSKIEECDVAQAAINLSQKNIQDKDKNIRTSHVGYYLIGN
                     GINQTKKSAGIQEPGIKRIFYFIKKNAFKAYLAIIIVITLAISTGIVDKASSETNNFW
                     LLLTIAILSLLCISQFAISVVNFFSTLFIKPDLLPRMDFSNKIPPEFSTLVVIPTILL
                     SKDKIENLVEALEVRFLANRNDNLHFGLLTDFKDSKQEKLSEDQSLIDLAQQKIEQLN
                     LKYERENNNLFYLFHRPRLWNAQENIWMGYERKRGKIYDLNSLLRGESKDKFSLIVGD
                     QSILPDIKYVITLDEDTQLPLGTAWKLIGTMAHPLNQAFYDEKKKLVTQGYGILQPRV
                     SASLPDTISSLYTKMHGNEPGIDPYTRVTSDVYQDLFGEGSFIGKGIYEVDIFRKVLE
                     FRFPENQILSHDLLEGSYARSGLLSDVQFFEKYPISYFSDMKRYSRWIRGDWQIF"
BASE COUNT         1419 a          599 c          738 g         1183 t
ORIGIN      
        1 aaaatagaat aataatattg taattatact tgtcatttat taacttaact ttaaatttag
       61 ttttagaaaa aatattagaa tataaaattg tttaattaaa aatttaaaaa tttaaaaata
      121 taaattataa tttaggtatg aaaacatttg tgaaaaaata taactcagca gtactttcgc
      181 ttttagttat gtttattatt gtaagctgct cttcaaaatc tcagaaagca gaagcagttc
      241 ttgagtccag atccggatca agtgtaacag gtaaagttac atttacaaaa gaaggaaacg
      301 ttgtaaagat tattgcagac attaaaggat taactcccgg aaagcacgga tttcatatcc
      361 atgaaaaagg ggactgcagc gctccggacg ccacatctgc aggcggacat tttaatccaa
      421 gatcaatgca gcattccggt catcaaacta ctgaaagaca taccggtgat atgggaaata
      481 taactgctga tgaatccggc aatgctcatc ttgagatatc agattccatt atgtcatttg
      541 atggtgaaga atctatcatt ggaaaaagcg tgatagttca cgaaaaagaa gatgatctca
      601 aaacacagcc gaccggtgat gctggcggta gagttgcatg cggagttatt gttattgtta
      661 aatagaaatc tgacaagagg atattgcaaa cattccggaa cacctgcaga agtttatata
      721 accaatgttg ctgaattttt taatatgcga taagtcaggg cagatcttaa gcatgatctt
      781 aaattcttct ccggaatttt gtcatccatt tgaaatacta ttgctctgtt gactttaaag
      841 tcatactttt tttaaaaaat aattttgaaa gtctccacta atctacaggt acatttgaaa
      901 aaaaatgcga attgacgggg agactttgat ttccagtcca ttctgaaagt actgccatta
      961 ttaaccagat aactcggttc atccaattca ggatgatgga ttactgtatt catttctttg
     1021 ctttacttta ctttacagga gaagaaagtg cggaagcttc attttcaaga tccgacgaaa
     1081 ctgtccggac tatcgataaa gatggatttg tacccgcttt gctttctgct gagatcacgg
     1141 caaagctgag ccttgtgatc cggggtttat atataatgaa ctcacaaaag aaatcggcag
     1201 aagcaaatcc tgtacaaaaa gaaacgatat agaaaatttc tcaaaatcaa ataattnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagca aatcctgtac aaaaagaaac
     1381 gatatagaaa atttctcaaa atcaaataat tcacaaaagt cttgcaggtg aaaaaattaa
     1441 aaacataatt tccgttgctg cctgataaga tgcttcaatt ggcggtgtaa aaggagaaac
     1501 aataaatggc tggtttacag cacggcattc ggataccaat gatatgtata aaatatatac
     1561 ataaagtttt ttgaattaag gtcatctgat tcaaattaac gatcagacac aggaaacttt
     1621 caactctgta ttaaaaaata atatataaat tttatgaaat ttgaattaaa agattacgaa
     1681 ttaatatcga atatgcggga attttatcaa ttaaatgaat ctgaccaaat tattacaaat
     1741 gaggaaccta taagagctga gttatacaat tcagcacaaa tggaacgatt tggaactacc
     1801 ttggcaggaa ctcacaaggt aagtacaatt ccctcaaagg attatttgct taagcgtctg
     1861 tcagataatg aaattaaatt acaagaagtt cgtaaactag ttacagattc aataaaaaag
     1921 aaattagaaa ttacctcaac aggagaatgg ctagtagata atttttacct gatagaggaa
     1981 aatattcata ttgcgaaaaa acatttcccc aaaaattaca gtgaaggtct gcctcactta
     2041 gttgatgaaa tatattcagg actgccaaga atttatgaca ttgcccttca aaacatttca
     2101 cattgtgacg gaataataga tattgaaatt ctgagcagat atataaaagc atatcaaaag
     2161 gatactaaat taaaattagg agaattgtgg gcattaccaa ttatgctccg acttgcattg
     2221 attgaaaatt taagacgggt tgctgtccgg gttgcggtga gcaggttaga tagaaatctt
     2281 gcagattttt ggtctaaaaa aatgatcgag actgctgaga attcacctaa agatcttatt
     2341 cttgtcattg ctgatatggc aagaacaaat cctccaatgg taagtggatt tgtttccgaa
     2401 tttatacgaa agctgcaagg acataaagca aacatgaatc ttgttcataa ctgggtggaa
     2461 aaccgattat cggaaatcgg tttatcaatt tcagaagtag tctatgcaga aaatcaaaaa
     2521 caagcagctc tgcagatttc aatgagtaat attataggca gtctccggtt gcttgacgat
     2581 acagactgga ggaattttgt ggaagaactt agtatagtcg aacaaactct gagggaagat
     2641 aagatatatg gattgatgga tttttctaca cgcgacagat acagacatgt agtggagcaa
     2701 attgctaaaa aaagtaaaat tgaagaatgt gatgtagctc aggcagcaat aaatttatcc
     2761 caaaaaaata tacaagataa ggacaaaaat atcaggacat ctcatgtcgg atattacctg
     2821 ataggaaatg gaataaatca aacaaaaaag tcagcaggta ttcaagagcc gggtattaaa
     2881 agaatttttt actttattaa gaaaaatgca ttcaaagctt atctggcaat tataattgta
     2941 attactcttg ctataagtac tggaattgtg gataaagcat cttcggaaac aaataacttt
     3001 tggctgcttt tgactattgc tatcctttca cttttatgta taagtcaatt tgcaatatca
     3061 gttgtcaatt ttttttctac tttatttata aagcctgatc tactgccaag aatggatttt
     3121 tcaaataaaa taccaccgga gttcagcaca ttggttgtga tccccacgat ccttttgagt
     3181 aaagataaaa tagagaattt agttgaggca ttggaagttc gttttcttgc aaacagaaac
     3241 gacaatttgc attttggatt attaactgat tttaaagata gtaaacagga aaagctttca
     3301 gaggatcagt cactgataga tttagctcag caaaaaattg agcaactgaa tcttaagtac
     3361 gaaagagaaa ataataattt gttttatctg tttcacagac ccaggctctg gaatgctcaa
     3421 gaaaatatat ggatgggtta tgaaagaaaa agaggtaaaa tttatgattt aaattctctt
     3481 cttaggggag aatcaaagga taaattttct cttatagtag gcgatcaatc cattttaccg
     3541 gatataaaat atgtgatcac attagacgaa gatacacaat tgcctcttgg tacagcatgg
     3601 aaattaattg gaactatggc tcaccctttg aatcaagcct tttatgatga aaagaagaaa
     3661 ctggtaactc agggatatgg aatccttcag ccccgggtaa gtgcgagttt accggatact
     3721 atcagttcac tttatacgaa gatgcatgga aatgaaccgg gtattgaccc atatacacgt
     3781 gttacttctg atgtatatca ggatttattt ggtgaaggtt cttttatcgg gaaaggtata
     3841 tacgaagttg acattttcag aaaagtactt gaatttagat tccccgaaaa tcagatttta
     3901 agtcatgacc tactggaagg gagttatgca aggtcaggat tactgagcga tgtacagttt
     3961 tttgaaaaat atcctatcag ttatttttcg gatatgaaaa ggtactcacg ctggattcgc
     4021 ggcgattggc agatctttc
//
LOCUS       DAORVQ010000256         4033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_17332_length_4033_cov_3.185772, whole genome shotgun sequence.
ACCESSION   DAORVQ010000256 DAORVQ010000000
VERSION     DAORVQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17332_length_4033_cov_3.185772"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..645
                     /locus_tag="PLX80_13670"
     CDS             127..645
                     /locus_tag="PLX80_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21884.1"
                     /translation="MILKKQISYVVILAIVFISYGTSFFYDDPFVTSEEINILTGEQW
                     TGNLTYLDYQTNSETRISANITVIRSIDNTNQYILILEYPKEPQANNIDTITISDDGK
                     KFSGENVIEKFSFDADSLKFITESFGMDDDKESIFRHTYDISQNKLKIRKDVRYDGDS
                     IFIKRNEFYFTR"
     gene            662..1132
                     /locus_tag="PLX80_13675"
     CDS             662..1132
                     /locus_tag="PLX80_13675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21885.1"
                     /translation="MDLKIRRAKESDTEEIGNMYFDTVNAVNSKDYPKEKIDVWAASG
                     KKYDMWKKKISEQYFLVAETGSIITGISSIDPTGYLDFMYVHKDYQRMGIAQALLNEI
                     EKKAAEQKNSEIWAYVSLTADPFFKKNGYEFSGEKIIVIQGVEFVDRILRKKLN"
     gene            1164..1691
                     /locus_tag="PLX80_13680"
     CDS             1164..1691
                     /locus_tag="PLX80_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21886.1"
                     /translation="MFLIIYQPGISVSQDKEELIEYIREELENIHLDTNLRSTTLINE
                     EFLPDMTDGGGELTGFYDENGISKIYRSVGISNGVGITEFYYKKNKLIFVREKFNSYV
                     FNENTKSFDYTKINTTYSGSYYFSKDKIFNSTVKGKRSFGDNLTDIQDILKKESDEAF
                     KRINESIKQVQDNKQ"
     gene            1706..2749
                     /locus_tag="PLX80_13685"
     CDS             1706..2749
                     /locus_tag="PLX80_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21887.1"
                     /translation="MLKIFTGFITILIILLFNTETKANVAQPGLWNAGGMGNFSLLYP
                     EDSISYKKIQMVDEKISIHLYKGFAVVKGVYKMYNETKDTVKIKTGYPLNGLFDGETD
                     NHKRAEISFDSLYGLDVKINNLPAEIISGPADGKTSKSYNDNWYVWENVFMPEDTTVI
                     TVYFIVNTNNTSIRQGYSKDHFNGFIYVLETGSTWKQPIKSGEIRIQTMDGILMKDIK
                     GIKPDSVFKINKTENGNILQYKFQNLSPVNADNIIITYSENLDNFNFENIIRGKENLY
                     KSIDVFSEMEINTSGLVNMKFDDPFEVVSTDWTSTLIILGIIGIPALIIISMIILLVI
                     FIKWYSKRRKNKI"
     gene            2756..3664
                     /locus_tag="PLX80_13690"
     CDS             2756..3664
                     /locus_tag="PLX80_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014654222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQY21888.1"
                     /translation="MNQKLKIALLQINSFNTDQRGNLIKGEEYCRMAKDLNADIVLFP
                     EMWNIGYESFNESAIETGFDPRNIPESIKNEVNLWTSNALEIDSEFVEHYRKLAGELD
                     IAIAITFLQKGKSKPFNSIAVIDKHGKIVLNYSKVHTCDFSMEYYCESGNEFLACDLD
                     TQSGPVRIGAMICFDREFPESARELMLLGAEIILIPNCCNMDSHRSSQLKTRAFENMT
                     GIALANYPAPKCNGRSQAYSPVVYDINGKELDNLILMADEEEGIFIAEFDMEKIREFR
                     KREVWGSNFRKPLAYKNLIKFKHDKT"
     gene            complement(3806..>4033)
                     /locus_tag="PLX80_13695"
     CDS             complement(3806..>4033)
                     /locus_tag="PLX80_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21889.1"
                     /translation="DYDTLILSGANDLFDDLKSPVLGIMGGLGIQINKVIPQTLIIEV
                     RYNADLTNSFKNSGLNYVRNYLWQFNIGIKL"
BASE COUNT         1495 a          598 c          719 g         1221 t
ORIGIN      
        1 atcaattttg agaatatgta tgtagatttt aaagattaaa tttatttaaa ttttttaaaa
       61 taactacatt attaaattct gatttgagat tataaactga atcattaaat taaaaaacta
      121 ttaaaaatga tattaaaaaa acagatcagt tatgtagtta tactggcaat tgtatttatt
      181 tcttacggga cttcattttt ttatgatgat ccatttgtta catcagaaga aataaatata
      241 ctaaccggag aacaatggac cgggaatctg acatatcttg attatcagac gaattctgaa
      301 acaagaattt cagctaacat tacagttata agatcaatcg ataatacaaa tcaatatatt
      361 ctgattctgg aatatcctaa agaacctcag gcaaacaata ttgatacaat cacaataagt
      421 gatgacggaa aaaaattcag cggagaaaat gtgattgaaa agttcagctt tgatgcagat
      481 tcactgaagt ttattactga atcttttggt atggatgatg ataaagaatc aatattcagg
      541 catacatacg acataagtca aaataaactc aaaatcagaa aagatgtcag atatgacggt
      601 gacagcatat tcataaaacg taatgaattt tattttacaa gataacttta attttttcaa
      661 aatggatcta aaaatcagaa gagctaaaga atcagacact gaggaaatcg gcaatatgta
      721 ttttgataca gtgaatgctg ttaactcaaa agattacccg aaagaaaaga tcgatgtctg
      781 ggctgcttcc gggaagaaat atgacatgtg gaaaaagaaa atctcagaac aatattttct
      841 ggtagcagag acaggtagta tcattaccgg tataagctca attgatccga ccggttattt
      901 agattttatg tatgttcaca aagattatca gaggatgggc attgctcagg cactgctgaa
      961 tgaaattgag aaaaaagcag ctgagcaaaa gaattcggaa atatgggctt atgtgagtct
     1021 tacggctgat ccttttttta aaaagaacgg gtatgaattt tcaggagaga agatcattgt
     1081 aattcagggt gttgaatttg tcgacaggat cttaagaaaa aaactaaatt agtgatatga
     1141 aattttttat caatttattt ttaatgtttc tgatcattta tcaaccgggc atttctgttt
     1201 cacaggataa agaagaactt attgagtata tcagagagga attagaaaac atacatcttg
     1261 atactaactt acggagcaca acacttatca atgaagagtt ccttccggac atgacagatg
     1321 gcggtggaga gctaaccggt ttttatgatg aaaatggtat cagtaaaatt tacagaagcg
     1381 tcgggatatc aaacggagtc ggtatcactg aattttatta taaaaagaat aaacttattt
     1441 tcgtcagaga gaaattcaat tcttatgtat ttaatgaaaa cacaaaatca tttgattata
     1501 caaaaattaa tacaacctac agcggaagtt attatttcag caaagacaaa attttcaatt
     1561 caacagttaa aggaaaaaga agttttggag ataatctcac ggatattcag gacattctta
     1621 aaaaagaatc agatgaagca ttcaaacgga taaacgaaag tataaaacaa gttcaagata
     1681 acaaacaata aattctaaaa ttaatatgtt aaaaatattt accggtttta taacaattct
     1741 gattattcta ctcttcaata cagaaaccaa agctaacgta gctcagcccg ggctctggaa
     1801 tgcaggcggt atgggaaatt tttctctgct ttatcctgaa gacagtattt catataaaaa
     1861 aattcagatg gtggatgaaa agatttccat tcatctgtat aaaggttttg cagtagttaa
     1921 aggtgtttat aaaatgtaca acgaaacgaa agatacggtt aaaataaaaa caggatatcc
     1981 gctgaacgga ctttttgacg gagagactga taaccataaa agagccgaga tcagttttga
     2041 ctctctttac ggactcgatg taaaaataaa caatttgcct gctgaaatta tatccggtcc
     2101 cgctgatggt aagacaagta agtcatacaa cgataactgg tatgtctggg agaatgtttt
     2161 tatgccggaa gatacaactg tcatcacagt atattttatt gttaatacca ataatacttc
     2221 aataagacag ggatattcaa aagatcattt taacggattc atatatgtgc tcgaaacagg
     2281 ttcgacctgg aagcagccga tcaaaagcgg agagattaga attcagacca tggacggcat
     2341 tcttatgaaa gatatcaaag ggatcaaacc tgactcagtt tttaaaataa acaaaactga
     2401 aaatggaaat attcttcaat ataagtttca gaacttatcg cctgtcaacg ctgataatat
     2461 tatcataact tattccgaga atcttgataa ttttaatttt gaaaatatta taagaggaaa
     2521 agaaaatctt tacaaaagca ttgatgtttt ttccgaaatg gaaatcaata cttccggact
     2581 tgttaatatg aaatttgatg acccgtttga agtggtgtca actgactgga caagtacact
     2641 tattatactt ggaataatcg ggatacccgc tttaataata atttcaatga ttattctttt
     2701 agtaatattt attaaatggt actctaaacg cagaaaaaat aagatttaat ttcagatgaa
     2761 tcaaaaacta aagatcgctt tacttcagat taattctttt aatactgatc agaggggaaa
     2821 tctgataaaa ggcgaagaat attgcaggat ggcaaaagat ctgaatgctg acatagtatt
     2881 atttccggaa atgtggaata tcggatatga aagttttaat gaaagtgcaa ttgaaacagg
     2941 ctttgatccc agaaatattc cggaatctat aaaaaatgaa gtgaatctgt ggacgtcaaa
     3001 tgctttggaa attgattctg aatttgttga gcattacaga aagctggcag gagaacttga
     3061 tattgcaatt gccataacat ttcttcagaa aggaaaaagc aaacctttta attcaattgc
     3121 tgtaattgac aaacacggaa aaattgtttt gaactattct aaagttcata cctgtgattt
     3181 ttctatggaa tactactgcg aatcgggaaa tgaattctta gcatgtgatc tcgatacgca
     3241 atccggacca gtcaggatcg gcgctatgat ttgctttgac cgtgaatttc cggaaagcgc
     3301 aagagagctg atgcttctgg gcgctgaaat aattcttatt cctaactgct gtaatatgga
     3361 ttctcacaga tcttcacagc tgaagacacg cgcttttgaa aatatgacgg gaatagcatt
     3421 ggctaattat ccggctccaa agtgtaacgg aagatctcag gcttattcac ctgttgttta
     3481 tgacattaac gggaaagaac tggacaatct gattttaatg gcagatgaag aagaaggaat
     3541 tttcatagca gaatttgaca tggagaagat ccgtgaattc agaaaaagag aagtgtgggg
     3601 aagtaatttc agaaagccgc ttgcttataa aaatctgatc aaatttaaac atgataaaac
     3661 ataaaatatg aattttattt atttctaata ttatcaaacc tgacccgctg atattaaact
     3721 ctcaggcgga tcaggaatga gatagttaaa cttcaatgcc ccgcataaaa tctgatatac
     3781 cacacaagtc tcaactcttt tcacattata gcttgatccc gatattaaac tgccatagat
     3841 aattcctgac ataatttagt cctgaatttt taaaactatt agtaagatct gcattgtatc
     3901 ttacttcaat tataagtgtc tggggaataa ctttattaat ctgtattccc aaacctccca
     3961 ttattcctag aacaggactt ttcaaatcat caaaaagatc atttgctcct gataaaatta
     4021 aagtatcata atc
//
LOCUS       DAORVQ010000257         4022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_17425_length_4022_cov_5.773380, whole genome shotgun sequence.
ACCESSION   DAORVQ010000257 DAORVQ010000000
VERSION     DAORVQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_4022_cov_5.773380"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1188
                     /locus_tag="PLX80_13700"
     CDS             196..1188
                     /locus_tag="PLX80_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY21890.1"
                     /translation="MKIILTVILLLTASHSFGQDSELKPLDLMLTYYDYPYAVNYLNF
                     SSQQQDLVMAYMDVKPDVFNGKTIMLLHGKNFNGAYWKTTIEALNNAGYRVIVPDQIG
                     FGKSSKPDHYQFTFQQLAKNTKLILDTLGIEKTFVLGHSMGGMLATRFALMYPETTEK
                     LILENPIGLEDWKTVVPYQTIDWWYQSELNSSYDKIKAYQLANYYDNKWKDEYDEWVN
                     LLAGWTLDPDYAKIAWNSALTYDMIFTQPVIYEFKDLTCPVLLIIGTRDRTALGKPLV
                     PEDVRKTMGLYDQLGKSTQKLIPNCELIEIQDIGHMPHIENFDAFIGPLLSFLN"
     gene            1329..1898
                     /locus_tag="PLX80_13705"
     CDS             1329..1898
                     /locus_tag="PLX80_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HQY21891.1"
                     /translation="MSKKIKKKLSLMQSNELLKILNARFEKNMNRHKGIDWTKIQIKL
                     EADSEKLWSLNEMEISGGEPDVVGFDKKTGEYIFYDCSAESPKNRRSLCYDRNAWESR
                     KEHKPKNNAMDMAKEMGIEILTEEQYRELQKLGNFDNKTSSWLNTPSDIRKLGGAIFG
                     DYRFGRVFVYHNGAESYYAGRAFRGCLKV"
     gene            1986..2462
                     /locus_tag="PLX80_13710"
     CDS             1986..2462
                     /locus_tag="PLX80_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002071109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY21892.1"
                     /translation="MTSEKFNSSKDFEIVDSRIVNASIDLVFKAWSDPEHLKNWWGPE
                     GFTNTFNEFDFRVGGKWSFIMHGPDKGNYHNECEFIKIEKPALIAWKRYSKPIFQVTA
                     AFEVISENKTKIVFKQIFDSSEECAKIKKYTAGKNDENFDRLEVELIKMITDIIAK"
     gene            2660..2968
                     /locus_tag="PLX80_13715"
     CDS             2660..2968
                     /locus_tag="PLX80_13715"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQY21893.1"
                     /translation="MILFFKASTGQINWKRGTPVRDNFEYNLKVNLIGNDIKQTGQKR
                     HLTQEESGKLISVQKAQISGLDSNASNVTIETLMKVFTAMKANVNFQIELSPGQNIST
                     "
     gene            3106..3945
                     /locus_tag="PLX80_13720"
     CDS             3106..3945
                     /locus_tag="PLX80_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY21894.1"
                     /translation="MGKNLNTNEPIMTSEQMEIKSIVECFLIIAGNYDIEKMEGIISE
                     KANIGISRLNEGEWQSTVITITEYFEDCKTRKLQPYYETVSEWKIDINEGQIAFVKAD
                     AILHIFGVPATRNQDYFTLIKENAKWKFLNLSFTKSLLHEDKKKFDIEIFAKSYSQAW
                     SGVRPEFVASFFEEEGSLQVNEGDPAKGRNEIADIANGFMTDLPDMIVRFDSLKNSPD
                     GIEFHWTLIATNSGPGGTGNNIEVSGYELWEMGENNLIKKSLGNFPSEEYERQISLND
                     SNK"
BASE COUNT         1489 a          594 c          730 g         1209 t
ORIGIN      
        1 ctttaaggta attagtgtta agaattttga ttataatgaa aggatcaagg ttacaaacaa
       61 aaataagaat aatactttat atcgtaacaa ctcgattata taaattcatc aggtaaaaat
      121 ttaattcata aaataaaaga acctatcttt taatgaaatc aaaataagta atttaaaata
      181 ataatcagct aacctatgaa aataatatta acagttatac tgctcctgac tgcaagtcat
      241 tcttttggtc aggacagcga actaaaacct cttgatctga tgcttaccta ttacgattat
      301 ccatacgctg taaactatct gaacttttcg agtcagcagc aggatcttgt aatggcttat
      361 atggatgtaa agcctgatgt ttttaatggc aaaacgatca tgcttcttca tggaaaaaat
      421 tttaacggag catactggaa aactactata gaagctttga acaatgccgg ttacagagtg
      481 atcgtgccag accagatcgg ttttgggaaa tcatccaaac ctgatcatta tcagtttaca
      541 tttcagcagc ttgcaaagaa taccaaatta attcttgaca cacttggcat tgaaaaaact
      601 tttgtcctcg gtcattcgat gggaggaatg ctggctacaa gatttgcgct gatgtatccg
      661 gaaactacag agaaactgat acttgaaaac cctatcggtc ttgaagactg gaagactgtt
      721 gttccatatc agaccataga ctggtggtat cagtcagaat tgaatagttc gtatgataag
      781 atcaaagctt atcagcttgc aaattattat gacaacaaat ggaaggatga atatgatgaa
      841 tgggtaaatt tacttgcggg ttggactctg gatcccgact atgcaaagat cgcctggaat
      901 tctgcgctga catatgatat gatcttcact cagcctgtaa tttatgaatt taaagatctg
      961 acatgcccgg tacttctcat aatcggaacc agagacagaa ctgcattagg aaaacctctt
     1021 gttccggaag atgtcagaaa aaccatggga ctttatgatc agttaggtaa atcaacacaa
     1081 aagctgatcc cgaattgtga attaattgaa attcaggaca tcggtcatat gcctcatatt
     1141 gaaaactttg atgcatttat aggtccctta ttatcatttc tgaattagct tctattttat
     1201 agatggcaag tttattgtga ctcatttatg aattcactga tgaaaatatt ttaattattt
     1261 ttaaaaaatt ttgaaattat atttttcatt taatgaaaat ctatattcaa taagaattaa
     1321 atactattat gtcaaaaaaa attaaaaaaa agttatcact tatgcagagt aatgagttac
     1381 tcaagatctt aaatgcacgt tttgaaaaaa acatgaacag acacaaagga attgactgga
     1441 ctaaaataca aattaagctt gaagctgatt cagaaaaact ttggtctctc aatgaaatgg
     1501 aaatatccgg cggtgagcct gatgttgtag gttttgataa aaagacagga gaatatattt
     1561 tctatgattg ttcagcggaa agtccaaaga accgcagaag tctatgctat gaccgaaatg
     1621 cctgggaatc aaggaaagaa cataagccca aaaataatgc aatggatatg gctaaagaaa
     1681 tgggaataga aattttaaca gaagagcaat accgtgaact tcagaagctt gggaattttg
     1741 ataataaaac ttcgagctgg ctgaatacac cttcagatat cagaaagctt ggcggtgcta
     1801 tttttggcga ttaccgtttc ggacgtgttt tcgtttatca caacggtgct gaatcatatt
     1861 atgcaggaag agcgtttcgc ggatgtttaa aagtttgatt ataaaatttc gtaataatta
     1921 gaaaatttat tttacattgg taaggatctt ttacaaccaa cattattttt aaaaataaat
     1981 tcgatatgac ttcagaaaaa ttcaattctt caaaagactt tgaaattgta gattcaagaa
     2041 ttgtaaatgc gtcaatagat ctggtgttta aagcatggtc agatccggaa catttaaaga
     2101 actggtgggg tcctgaaggt tttacaaata cttttaatga atttgatttt cgtgtaggag
     2161 gaaaatggag ttttatcatg catggtcctg acaagggaaa ttatcataat gaatgtgagt
     2221 ttattaaaat tgaaaagcct gcacttattg catggaagcg atattcaaaa ccaatttttc
     2281 aagtaactgc agcttttgag gtaatctctg aaaataaaac aaagatagtt ttcaaacaga
     2341 tctttgattc atcagaagag tgtgctaaaa taaaaaaata tacagccggt aaaaacgatg
     2401 aaaactttga cagacttgaa gtcgaattaa taaaaatgat tacagacatt atagctaaat
     2461 gaaaaaatca aactataatg gttgtttaca attaaggaaa caagctttat ttttgttaag
     2521 attttttgaa tataattatg acaacgtatg aaaacttttc gaagtactct ttttggaaga
     2581 agcttataaa ttttacaaat ttttgataaa aaacattcag aaaagatact ttataacatt
     2641 cgaaaatcgc agtttgagta tgatcttatt ctttaaagca agtacaggac aaattaattg
     2701 gaaaagagga actcctgtca gagacaactt tgagtataat ttaaaagtga acttaattgg
     2761 taacgatatt aagcagaccg gacaaaagag gcatttgaca caggaagaat caggtaaact
     2821 tattagtgtt cagaaagctc agatatccgg acttgacagt aatgcaagta atgtaaccat
     2881 tgaaactttg atgaaagttt tcacagctat gaaagcaaac gttaattttc aaattgaact
     2941 ttcccccgga cagaacatca gtacatgata agtgctttta atattcataa attaaagaaa
     3001 attatacaaa gcaatttcat tcaatattat tattagtagt aataccatta gaatatatat
     3061 tctgaatgac tattctgaca attcaaccaa acaatttaat attaaatggg taaaaattta
     3121 aacacaaatg aaccaatcat gacatctgaa cagatggaaa taaaatcaat tgtcgaatgc
     3181 tttctgataa ttgcgggaaa ttatgacata gaaaaaatgg aaggaataat ctctgaaaaa
     3241 gcgaacatcg gtatcagcag acttaatgaa ggtgaatggc aaagcaccgt cattacaatt
     3301 acagaatatt ttgaagactg taaaacacga aagttgcagc catattacga aactgtaagt
     3361 gaatggaaaa tagatatcaa cgaagggcaa attgcatttg taaaagccga tgctatactt
     3421 cacattttcg gagtgccggc aacccgaaat caagattact ttacacttat caaagaaaat
     3481 gcaaaatgga aatttctgaa cctttcgttt acaaaaagtt tattacatga agataaaaag
     3541 aaatttgata ttgaaatatt tgcaaaaagt tattcgcagg catggagcgg agttcgccct
     3601 gaatttgtag cttctttttt tgaagaagaa ggatctcttc aggtgaatga aggtgatcct
     3661 gccaaaggca gaaatgagat tgcagatatt gcaaatggtt ttatgacaga tcttccggac
     3721 atgatagttc gttttgacag ccttaaaaat agtcccgacg gaattgaatt tcactggaca
     3781 ttgattgcca caaactcagg acctggcggc actggcaata atattgaagt cagcggatat
     3841 gagctttggg aaatgggaga aaataatctg atcaaaaaat cattgggaaa ttttccttcg
     3901 gaggagtacg agaggcagat aagtttgaat gacagcaaca aataataatt ttctacagct
     3961 tttcacactt cactttccga tacagaaatt tctaaaaatg ttatttaaaa tatcatcatt
     4021 gg
//
LOCUS       DAORVQ010000258         3954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_17975_length_3954_cov_6.724545, whole genome shotgun sequence.
ACCESSION   DAORVQ010000258 DAORVQ010000000
VERSION     DAORVQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17975_length_3954_cov_6.724545"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..778
                     /locus_tag="PLX80_13725"
     CDS             179..778
                     /locus_tag="PLX80_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HQY21895.1"
                     /translation="MDIAFGILLGIGLSAAAGFRVFLPLLVTNIVSMLGYINLTGGFE
                     WMGGWIAFTAFLVAAIVEISAYYIPWLDNLLDTISGPLAMVAGTVLTASFLTEANPLL
                     QWVLGIIVGGGTAGIVKVGASAARLTSTATTGGAGNAVIATGENVMSVVVAILSFIIP
                     FITAFLVFLIVIYLIRKFIAIKKSGFFEREEKISEITEN"
     gene            complement(784..1224)
                     /locus_tag="PLX80_13730"
     CDS             complement(784..1224)
                     /locus_tag="PLX80_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQY21896.1"
                     /translation="MNNIQKLYISELDALAKEISLYRDEKNLWILTGDIKNSPGNLCL
                     HIIGNLKHFWGNIIGGTGYVRERDKEFSDKNISRDELIKGIEETKIVLNNIFENLSDE
                     DQQKPYPEDFFGKGSNVAFVTARLISHLSYHLGQINYHRRITEG"
     gene            complement(1243..3675)
                     /locus_tag="PLX80_13735"
     CDS             complement(1243..3675)
                     /locus_tag="PLX80_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQY21897.1"
                     /translation="MKKQTKLSLNGKTSFGEYKKEVLADYKLANESRQASILGRKEVL
                     TGKAKFGIFGDGKEVAQIAMAKAFQNGDFRSGYYRDQTFAFATGISTVKEFFAQLYAD
                     TNVKLEPASAGRSMNGHFANRLLDDKGNWKNLMEMKNTSADISPTASQMIRLVGLAYA
                     SKLYRNNEQLHGMTDFSDKGNEVAFGTIGNASCAEGMFFEAINAAGVLMIPMAVSVWD
                     DGYGISVPSKYQITKENISEILKGFEYDEKKDQGYLIYSAKGWDYPELTEMYRDGISQ
                     VREKHIPALFHITEVTQPQGHSTSGSHERYKSKERLEWETEYCCIVQMRKWMLANAIG
                     TEEELDAIESDAKDYAKSMQKEAWKDFLDPIKKEQFEVIEMLDRISGDDKIEKEIKAI
                     SDKLKRSLDNNRKEISESINTVLRLVRNQKGGSFETVDLLKTWYRNFRIEYNRTYDSH
                     LYSQSEFSAMNVKEVKPVYSSDSKTLDGREVLNAFFDIAFDRDPRIFAIGEDLGKLGD
                     VNQGMAGLQEKYSELRLTDTGIREATIAGQGIGTALRGLRPIIEIQYLDYLFYALQIL
                     SDDLACLQYRTAGGQKAPLIIRTRGHRLEGIWHSGSPMGTIINSVRGIHVCVPRDMTR
                     AAGFYNTILKSDEPAIIIERLNAYRLKEDLPDNLGEVCVPLGVPEILAEGNDITIVTY
                     GACVDIARDAVSKLAGVNISCELIDVQTLLPFDINGMIAESLKKTNRILFLDEDVPGG
                     ASAFMMQNVIDRDGGYRYLDSPPKCLAAKEHRPAYGTDGDYFSKPNAEEIFETVYDIM
                     HEADPGQYPDFL"
BASE COUNT         1109 a          815 c          714 g         1316 t
ORIGIN      
        1 ggctcaatgg cttaaaggct caatggctta aaggctcaat ggcttaaagg ctcaaaggct
       61 taaaatttga aaccgttcca aaacagagtt tcgatttcaa ttacgtttcc aaaaaagagt
      121 ctggtaacgc gcatcaaatg atttatgatt cataattttt aattcaaaat tatcaaaaat
      181 ggacattgca ttcggtattt tactcggcat aggattatca gcggctgcag gattcagggt
      241 attccttccg ttactggtca ctaacattgt atcaatgctc ggttatataa atctgaccgg
      301 cggatttgaa tggatgggcg gatggatcgc attcacagca tttttagtgg cagcgatagt
      361 cgaaatttcc gcttactata ttccatggct cgataattta ctcgatacaa tatcaggtcc
      421 attggcgatg gtagccggaa cagtccttac tgcgtcattt cttacggaag caaatccatt
      481 acttcaatgg gttctcggaa ttatagtcgg cggaggaact gcaggtattg tgaaagtcgg
      541 agcaagcgct gcaagactta catctactgc aacgacagga ggagcaggaa atgctgtaat
      601 tgctacaggt gaaaatgtta tgtcagttgt agttgcaatt ctgtctttca taattccatt
      661 tattactgct ttccttgtat ttctgatcgt gatttatctg atcagaaaat ttattgctat
      721 aaaaaaatca ggtttctttg aaagggaaga gaaaataagt gagatcacag aaaattaata
      781 aatttaacct tctgtgatcc gtctgtgata attgatctgt ccgagatgat aagataaatg
      841 agatatcagt cttgccgtta caaaagcgac atttgaacct tttccaaaaa aatcttcggg
      901 gtaaggtttc tgctgatctt catcggataa attttcaaat atattattca aaacaatttt
      961 agtttcttca atacctttta taagttcatc cctgctgata tttttatcag aaaattcttt
     1021 atctctttct ctgacatatc ccgtcccgcc aataatattt ccccaaaaat gttttaagtt
     1081 tccaatgatg tgaagacaca gatttcccgg agagtttttg atatcaccgg taaggatcca
     1141 taaattcttt tcatctctgt acaaagatat ttctttagcc aaagcgtcaa gctctgaaat
     1201 gtaaagcttc tgaatattat tcatatttgg aaataaatta ttttacaaaa agtcagggta
     1261 ctgtcccgga tctgcttcat gcattatgtc ataaacagtt tcaaagatct cttcagcatt
     1321 cggctttgag aaataatctc cgtccgtacc gtaagcgggt ctgtgctctt ttgcagccag
     1381 acacttcggc ggagagtcaa gatatctgta gccgccgtca cggtcgatca cattctgcat
     1441 catgaatgca gatgcgccgc cgggaacgtc ctcatccagg aacaggatcc tgtttgtctt
     1501 ttttaatgac tctgcgatca ttccattaat atcaaaaggt agaagagtct gtacgtctat
     1561 gagttcacat gaaatattta caccggcaag ttttgaaaca gcatctctcg caatatcaac
     1621 gcaggcgccg taagttacta ttgtaatgtc atttccttct gcgagaattt caggcacacc
     1681 gagaggaacg caaacttcac caagattatc gggaagatct tctttaagtc tgtaagcatt
     1741 taatctttca ataatgatgg caggctcatc agacttcaga atcgtattgt aaaaccctgc
     1801 agctctggtc atgtctctcg ggacgcagac atgaattccc cttacgctgt ttatgatcgt
     1861 acccattggt gaacccgaat gccagatacc ttcaagtctg tgaccgcggg tcctgataat
     1921 aagcggagct ttctgaccgc ctgcagttct gtattgcaga caggcaagat catcacttaa
     1981 aatctgtaat gcataaaata aataatcgag atactgaatt tctatgatag gtctgagccc
     2041 tctgagtgca gttccaattc cctgaccggc gatggtcgct tctctgatgc ctgtatctgt
     2101 aagacggagt tcggaatatt tttcctgaag tcctgccatc ccctgattta catcaccgag
     2161 ttttccaagg tcctcaccga tcgcaaatat tctcggatct ctatcaaatg caatatcaaa
     2221 aaatgcattc aggacttcac gtccgtcaag ggtttttgaa tcggaagaat atacaggttt
     2281 tacttctttt acattcatag ctgaaaattc agactgactg tataaatgcg aatcataagt
     2341 tctgttatat tctattctga aatttctgta ccaggtcttt aaaagatcca ctgtttcaaa
     2401 tgatcctcct ttttggttac gtacaagtct gagtacagta tttatagatt cagaaatttc
     2461 tttgcggtta ttatcgagtg atctttttaa tttatctgaa atggctttga tctctttttc
     2521 aattttatca tcacctgaaa ttctatcaag catttcaata acttcaaact gttccttttt
     2581 gatcggatcc agaaaatctt tccaagcttc cttctgcata ctttttgcat aatcttttgc
     2641 atcagattct attgcatcga gttcctcttc tgtaccaatt gcatttgcca gcatccactt
     2701 tctcatctgc acaatgcagc agtattcagt ttcccattca agtctttctt tggatttata
     2761 tctttcatgt gagcccgatg tagaatgtcc ctgcggctgc gtgacttctg tgatgtgaaa
     2821 taatgcggga atatgttttt ccctgacctg tgaaattccg tctctgtaca tctctgttaa
     2881 ttccggataa tcccagccct ttgctgaata tataagatac ccctgatctt tcttttcatc
     2941 atattcaaaa ccttttagta tttcggaaat attttccttg gtgatctgat atttgcttgg
     3001 aacggaaatt ccgtaaccgt catcccagac tgaaactgcc attgggatca tgagtacacc
     3061 cgcggcattt atagcttcga agaacattcc ctctgcgcag cttgcatttc ctatcgttcc
     3121 gaatgcaact tcatttcctt tatcagaaaa atccgtcatg ccgtgaagct gttcattatt
     3181 tctgtaaagc tttgaagcat aagcaagtcc gacaagtctt atcatctgac ttgcagtcgg
     3241 tgaaatatcc gcagaagtat ttttcatttc catcagattc ttccagttac ctttgtcgtc
     3301 taaaagtctg tttgcaaaat gtccgttcat cgaacgtccg gctgaagccg gttcgagttt
     3361 tacatttgta tcggcataaa gctgtgcaaa aaattctttc acagtagata tcccggtggc
     3421 aaaagcaaat gtctgatccc tgtaatatcc tgatctgaaa tctccgtttt gaaatgcctt
     3481 tgccatagcg atctgagcta cttcttttcc gtcaccgaat atgccgaatt ttgctttgcc
     3541 tgtaagtact tcttttcttc cgagaatact tgcctgcctg ctttcatttg caagcttata
     3601 atcagctaaa acttcttttt tatattcacc gaatgatgtc tttccgttta aggatagttt
     3661 agtttgtttt ttcattatct tttgaattct ttgtaagaga tgaatttact gattattgcc
     3721 taaaggttaa atataaaatt atgactctta ttaaaaacta tatgcttaat ttcttttgat
     3781 ctatcccccg ataggtgcag attgaccgaa aacgatggaa ttacctctaa ggctctaaga
     3841 ctctaaagct ctaagactct aaggctctaa gactctaagg ctctaagacc ctaaggctca
     3901 aagtttaaaa agtttaaaaa gtttaaaaag tttaaaaagt ttaaaaagtt taaa
//
LOCUS       DAORVQ010000259         3811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19200_length_3811_cov_4.116347, whole genome shotgun sequence.
ACCESSION   DAORVQ010000259 DAORVQ010000000
VERSION     DAORVQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19200_length_3811_cov_4.116347"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="PLX80_13740"
     CDS             <1..2044
                     /locus_tag="PLX80_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY21898.1"
                     /translation="KWASSFLKEIQKSKMRRIKLKQLLLLALRIGIITLLVLTFAKPV
                     YEGYSFNNDAGGKSTVLIFIDDSFSMDASDVSGQYFSQAKNSVKEILNNYKESDEIIL
                     IPSSFTGYKNSIGINGGKKEITDSLEQLKLSNKPFYFNDALIKAGQILDNSINPNKDV
                     FVISDFQRINGGVSASEIKTENYEDVNFILINTGERTSVNLSLDSFKVNTKIPAKFGN
                     IRVSVNLSNHSPDPVSGISLKLFLNDKLTEETSADVSANSSNRKEFSFTAEKAGDLHG
                     FIELSGNSLQNDEFDQDNRIYFDVFIPEKINVGMIGNSNEFRFINTVLNSAQDLSGDN
                     AENNDNELFEINLSDRLDESIFSNDVLLISSGNNYSDRESELLSEFLTAGKGIFIFPN
                     EYTDIQNFNSEISSKLYIPQIGSFNTDENANKNLKFGKIDFENPIMSEIFRNNQLNIT
                     SDSFLDSPGIKKFYELLLNDKSIPVIMLSDGKPFITETELPKGKMLLSAVSGETGFSD
                     FPLNNIFPPLIIRSIFFLNSDFGFSNENYIGASNIIPVKGLNNIFSITTPENNTIELN
                     TELSGSSNDFYILPYTETAKDKGLYLLKDSSGNQFGVSLNIDSRESDPGRMDDEEIVK
                     YFNNAGIADAEIISNTNEISDLINNKRTGTSLWKFFLIAALILLAAEIFYSKKLEE"
     gene            complement(2292..>3811)
                     /locus_tag="PLX80_13745"
     CDS             complement(2292..>3811)
                     /locus_tag="PLX80_13745"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQY21899.1"
                     /translation="YDGLMGNESPEEYDIFVKTLNEFLERFHLIKKDELSPEDKYELD
                     ASIWSIEAALFDVEKIQSHKKNPMHYAFTFSGLHNYISREYAPFDDRLRSVVNIMKKI
                     PEVLKNAETNLDKVLPGVLCRYARHFSQGYEDFFNVELLNVVSERCNDDELKKEYILA
                     GENAAKAFNRYIEFLDKAGSDDDKSYIFGKEKFMDMLKVKEHIDFNFEELKSNGQKEL
                     ERLQFELKKILDENDFHDKLDSLEHDHPSESSLVSDTENTLNELIEFIRKNDIVNLPD
                     ELNCIVTEMPRYMNFGFAAMNTAGPYEKSNESFYYVNLPEKEWDEKKKEEWLTQFNYP
                     ILKLISIHEAYPGHYTHFLNSNLFSSDISKLFMSYSYVEGWAHYTEEMMIDQGYSKGD
                     FKAKIGMLLEALIRCCRYNVAIGIHCEGLTIDEAKEYFMKNANMVETTALQESERGAF
                     DPGYINYTLGKILIKKFKEDYFEKFGESKSLKDFHDMIVSLGCPTYRIARELVLA"
BASE COUNT         1298 a          619 c          596 g         1298 t
ORIGIN      
        1 aaaatgggcc tcttcgtttc taaaggaaat tcaaaaatcc aaaatgagaa ggattaagct
       61 gaaacagctg ctgcttctcg cattaaggat cggcattatc actttacttg ttctgacttt
      121 tgcaaaacct gtttatgaag gttattcatt caataatgat gcgggcggta aatcaactgt
      181 tctgattttt attgatgatt catttagtat ggatgcaagt gatgtatccg gacagtattt
      241 ctctcaggca aaaaattcag taaaggaaat tctaaacaat tataaagagt ctgacgagat
      301 cattcttata ccttcatcgt ttacaggtta taaaaattct attggaatca acggaggcaa
      361 aaaggagatc accgattctc ttgaacagct taagctttcg aataaaccat tttattttaa
      421 tgatgctctg attaaagcag gacagatatt ggataattcg attaatccta ataaagatgt
      481 atttgtaata tcagattttc aaagaattaa tggaggggtt tcagcatctg aaattaagac
      541 agaaaattat gaagatgtaa attttattct gataaataca ggtgagcgga cttcggtaaa
      601 tctttcttta gacagtttta aagtaaacac aaagatacct gcaaaatttg gaaatatcag
      661 agtctctgtt aatctgtcaa accactctcc ggacccggtt tcagggattt ctttaaaact
      721 ttttttaaat gataaactga cagaggaaac atctgctgat gtaagtgcaa acagcagtaa
      781 cagaaaggag ttttcattta cagcagagaa ggcaggtgat ctgcatggct tcattgagct
      841 ttcgggtaac agcttgcaga atgatgaatt tgaccaggat aacaggatct actttgatgt
      901 ttttattccc gaaaagatta atgttggaat gattggaaat tcaaatgaat tcagattcat
      961 taatacagta ctgaattctg ctcaggatct gtcaggagat aatgctgaaa ataatgacaa
     1021 tgaattattt gaaattaatt tatctgacag attggatgaa agtatttttt caaatgacgt
     1081 acttctgatt tcatctggta ataattattc tgacagagag tcagagctgt tatcagaatt
     1141 tttgactgca ggaaaaggga tctttatttt tccaaatgaa tatactgaca ttcaaaattt
     1201 caattctgaa atttcatcaa agctatacat cccgcagatt ggatctttta atactgacga
     1261 aaatgcaaat aaaaatctta agttcggaaa aattgatttt gagaatccga ttatgtcaga
     1321 gatattcagg aataatcagt tgaatattac atcagactct ttcctcgatt cacccggtat
     1381 aaaaaagttt tatgaattat tattaaatga caagagcatt cctgttataa tgttatcaga
     1441 tggaaaacct tttataactg aaactgaact gcctaaaggg aagatgcttc tcagtgcagt
     1501 atctggtgaa accggatttt ctgattttcc tttgaataat attttcccac cgctgattat
     1561 cagaagtata ttttttctga acagtgattt tggattttca aatgaaaatt atattggtgc
     1621 atcaaatata attcctgtaa aaggtctgaa taatattttt tcaattacta ctcctgaaaa
     1681 taatacaata gaattgaata ccgaactttc aggatcttca aatgactttt atattttgcc
     1741 atatactgaa actgcaaaag acaaagggct ttatctttta aaagactctt caggaaatca
     1801 atttggtgtg tcacttaata ttgattcaag agaaagtgat ccgggcagga tggatgatga
     1861 agaaatagtg aaatatttta ataatgcagg aattgcagat gctgaaatta taagtaatac
     1921 aaatgaaata tctgatctca ttaataataa aaggaccgga acttcacttt ggaaattctt
     1981 tctgatagct gcattgatat tacttgctgc agaaatattc tactctaaaa aattagaaga
     2041 gtaaattttt ttattagatt ttatttaatc tcttaccata caaattaatc gcaaaatagt
     2101 aagaaaattt catgtgaatc taattgagat aaatttctga attttaaata gtacgattta
     2161 gaataatttg aaattttttc cgccttaaaa aaggggatcg aagggggttg tagccgaaag
     2221 aagaaacaat caaaatcttt cagaggactc tcattcaaag aagtaagaag aataataaat
     2281 gtctaagttt tttaagctaa taccagctct ctggcaattc tgtaagtcgg acatccaagt
     2341 gaaactatca tatcatgaaa atcctttaga gatttactct caccaaattt ttcaaaataa
     2401 tcctctttga atttttttat aagaattttc ccaagagtat aattaatata acccggatcg
     2461 aatgcgcctc tttcggattc ctgtaatgca gtagtttcta ccatgtttgc attcttcata
     2521 aaatattcct ttgcttcatc gatcgtcaag ccttcacagt gaataccgat tgcaacatta
     2581 tatctgcagc atctgatcag agcttcaagc aacattccga tctttgcttt gaaatctcct
     2641 ttggaatatc cctgatcaat catcatctct tctgtataat gagcccaacc ctctacataa
     2701 gaataactca taaatagttt tgagatatca gacgaaaata aatttgaatt aagaaaatgt
     2761 gtataatgac ccggatatgc ttcatgaatt gagataagtt ttaatatcgg ataattaaac
     2821 tgtgtaagcc attcttcttt tttcttttcg tcccattctt tctcaggaag atttacataa
     2881 taaaatgatt cattcgattt ttcataagga ccggcagtat tcattgcagc aaatccaaaa
     2941 ttcatatatc tcggcatttc agtaactata caattcagct cgtcaggtaa atttactata
     3001 tcattttttc tgatgaactc tatcaattca ttgagagtat tttcagtgtc tgatactaat
     3061 gaactctcgg acggatgatc atgctcaagt gaatcgagtt tatcatggaa atcattttca
     3121 tcaaggatct ttttaagttc aaattggagt cgctcaagtt ctttttgtcc atttgatttt
     3181 aattcttcaa aattaaaatc aatatgttct ttcactttca gcatatccat aaatttctct
     3241 ttgccaaaaa tgtaagattt atcatcatcc gaaccggctt tatctaaaaa ttcaatataa
     3301 cgattaaatg cttttgctgc attttcacct gcaagtatat attctttttt taattcatca
     3361 tcattacatc tttcgctgac aacattaagt aactcaacat taaaaaaatc ttcataaccc
     3421 tgagaaaaat gtctcgcata cctgcataaa actccgggta aaactttatc aagattagtt
     3481 tcagcatttt ttaaaacctc cggaatcttt ttcataatgt tcacaactga ccttaatcta
     3541 tcatcaaatg gtgcatattc acggcttata taattatgaa gtccgctgaa agtaaatgca
     3601 tagtgcatag gatttttttt gtgagactga atcttctcta catcaaacag agcagcttct
     3661 attgaccaaa ttgatgcatc aagttcatat ttatcttcag gtgaaagttc atcttttttt
     3721 attaaatgaa accgctccaa aaattcattc aaagtcttaa caaatatatc atattcctca
     3781 ggtgattcat tacccattaa accgtcatat t
//
LOCUS       DAORVQ010000260         3799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19305_length_3799_cov_4.957799, whole genome shotgun sequence.
ACCESSION   DAORVQ010000260 DAORVQ010000000
VERSION     DAORVQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19305_length_3799_cov_4.957799"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..546
                     /locus_tag="PLX80_13750"
     CDS             325..546
                     /locus_tag="PLX80_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21900.1"
                     /translation="MKNLIFAICFLMVSCLPINIKAEKSTKKFINVNKINKYYQTSDY
                     TYELVFKDGIWWIFVYDEDGVFVTAYPSE"
     gene            709..1086
                     /locus_tag="PLX80_13755"
     CDS             709..1086
                     /locus_tag="PLX80_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21901.1"
                     /translation="MKKIIVIASFVLIALFTVFAINDNLLSKDTSENPAPTFEVTVYQ
                     TGGSTVQANTEVVVYDANANIVESGTTLSNGKVSWSWNHGYGNYTVKAWYPARPLDGQ
                     SAQTIVNYSGSSIYTSVTLGPNY"
     gene            1147..2862
                     /locus_tag="PLX80_13760"
     CDS             1147..2862
                     /locus_tag="PLX80_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21902.1"
                     /translation="MNKIITLILLIIFITGSDIISQTPYNYIDPIKIDTPRSPFRKAG
                     IPLITDLEKDGMKEIIVFTVDYNAAAIPLCNLHVSNYDGSNYPNFPIGMQDYALDIAS
                     GDINGNGYLDIAIRFTHRIDVIDRFGNSLPGFPLNYSDGDENPVKFISIYDLDNDNKL
                     EIIVSKNREICVFNFDGSIRSGWPRSISGTSKYNPAIGDIDGDGFGEIILNSFGNYNS
                     TVDSAAITIFRHNGILFNENWPIMLDSNYHSWSSSPSLYINKNNPDLTFILIAAGRLI
                     DGSSFGNHLLMKLNINGKVLDKKYYSDYMNFGTLVMGDLDRDNILEFVSGTQYGITLS
                     AFKNSLTKIEGWPRFGVGEQEATAVIGKLTFANELNIINNRWSADQGFGFIQSYTKDG
                     IDLPWSPLRPIGLVKAVSLADLNNDGNVEMISTSTKTLNETYLHIWTIPGIPYTHEDF
                     PWPQYGHDRYRTNQFGFIPPDEPVGIQPYSTTVPEKFSLSQNYPNPFNPSTVINYELR
                     VTGFTKLIVYDVLGHELATLVNEKQIAGSYRISFDGSNLSSGVYFYKLDAGEFSETKR
                     MILLK"
     gene            complement(3128..>3799)
                     /locus_tag="PLX80_13765"
     CDS             complement(3128..>3799)
                     /locus_tag="PLX80_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21903.1"
                     /translation="SYFCIENEHWRVICLDTGYNSVGIPLLEYIFKPKSEFRKEILKW
                     LKEEVKINEDKRGLIFMTHHQYCTAFEKEDEYVKPAEQLAELIGKDRPVIWIWGHEHR
                     LTMFGKYSSENGIAAYGRCIGNGGMPIEFKNKILPDMDKKRNLILYDERVYTKIKGFD
                     IGYNGFTIMSIKDKELTLEYFDIKDEKLITEKWTADNQTGEIDNMEFTVHSKDDGIIQ
                     LGIKN"
BASE COUNT         1288 a          558 c          633 g         1320 t
ORIGIN      
        1 tatttccata aaaatattta caattttacg gctcttattg tgatatggtg atttgataaa
       61 cttttctaag cgatttaatt cggatggaga aaattctttc aaaagttgaa taattgcatg
      121 ttcaaacata gtttttttaa ttttaaaagc gtcagtgata tttttaaaat attgatttct
      181 gtttaaaaat tcaaattaat ttcggtgttt taggaaatta acatatcaaa aaaagcgtca
      241 aaccggaaaa agtgtcccta acatcttgct ttaaattcta ataatattgt atagaattta
      301 aaattaaaaa ttaaaggagg aacaatgaaa aacctaattt ttgcaatttg ttttttaatg
      361 gttagctgtc tgccaattaa tattaaggcg gaaaaaagta ctaagaaatt tattaatgtt
      421 aataaaatta acaaatatta tcaaacatca gattatactt atgaactggt tttcaaggat
      481 ggaatttggt ggatatttgt ttatgatgaa gatggagtat ttgtgactgc atatccatct
      541 gaataaagaa agtttaatta tttgaaatta atttaaaaaa gaaaggagga tatcataatg
      601 ataagatcac catataatta taccactaaa attaaagagg ataaatataa attagaacat
      661 ttttaaaaag ttatatttta attaaaatta aaagaaagga aatttaaaat gaaaaagatc
      721 atagtaatag catcatttgt tttaatagca ttgtttactg tatttgcaat taatgataat
      781 ttactgtcca aggatacttc ggaaaatcca gctcctacat ttgaggtgac agtttatcag
      841 actggtggtt ctacagttca ggctaatacg gaagtagttg tatatgatgc aaatgcaaac
      901 atagtagaaa gtggcactac cttaagtaat ggtaaagtaa gctggtcatg gaatcatgga
      961 tatgggaatt atactgttaa agcttggtac cctgccagac cacttgacgg tcagtcagca
     1021 caaactattg tgaattattc cgggtccagt atatatacat ctgtgacact aggtccaaac
     1081 tattaatata attacaggca ggttaatttc ctgcctgttt aattattatt caaatattta
     1141 aatctcatga ataaaatcat aacattaata ttattgataa tttttattac cggatcggat
     1201 atcatatctc aaacaccata caactatatt gatccgataa aaattgatac gccgaggtct
     1261 cctttcagaa aagcaggtat accattaatt acagatcttg aaaaagatgg aatgaaagaa
     1321 ataatcgtgt ttacggttga ctataatgct gctgctattc cactttgtaa tttacatgta
     1381 tcaaattatg atggcagtaa ttatccaaat tttccaattg ggatgcagga ttatgctctt
     1441 gacattgctt ctggagatat taatggtaac ggatatttag atatagcaat taggtttact
     1501 catagaattg atgttattga cagattcgga aatagtttgc ctggttttcc tttaaattat
     1561 agtgatggag atgaaaaccc agtaaaattt ataagtattt atgatcttga taatgataat
     1621 aagcttgaaa taattgtaag caagaataga gaaatatgcg tttttaattt tgatggaagt
     1681 ataagatcag ggtggccaag atctatatca ggaacatcaa aatataatcc ggctattgga
     1741 gatattgatg gagatgggtt tggagaaata atattaaatt ctttcggaaa ttataattca
     1801 actgtagatt ccgcagctat aactattttt cggcataatg gtattttatt taatgaaaat
     1861 tggccaataa tgttggattc caattatcat tcatggagtt catcaccttc tttatatata
     1921 aacaaaaata atccagattt aacttttata cttattgctg ctggtagatt gatagacggt
     1981 agttcttttg gaaatcattt gctgatgaaa ttaaatatta atggaaaggt gttggataaa
     2041 aaatattatt ctgattatat gaatttcgga actttagtaa tgggtgatct tgatagagac
     2101 aatattttag aatttgtatc aggaactcag tatggaatta ctctctcagc ttttaaaaat
     2161 agtctgacaa agattgaagg atggcctcgt tttggtgttg gggaacaaga agcaacggct
     2221 gttataggaa aattaacttt tgcaaatgaa ctaaatatta ttaacaacag atggtcagca
     2281 gatcaaggct ttggttttat tcaatcttat acaaaagatg gaattgatct accgtggtcc
     2341 cccttgagac caatagggtt agttaaagca gtttcattag cagatttaaa taatgatggt
     2401 aatgtggaaa tgatttcaac ttcaactaaa actctcaatg aaacatatct tcatatctgg
     2461 actataccag gaattcctta cacacatgaa gattttccat ggcctcaata cggtcacgac
     2521 cgctatagaa caaatcaatt cggctttatc ccgcccgacg aacctgtagg aattcaacct
     2581 tattctacta cagtgccgga gaagttttca ctgtcgcaga attatccaaa tccatttaat
     2641 ccaagtacag ttataaatta cgagttacga gttacgggtt ttacaaagtt gattgtatat
     2701 gatgtactcg ggcatgaatt agctacgctt gtgaatgaaa agcaaatagc cggaagttac
     2761 agaataagtt ttgacggaag taatttatcg agcggagttt atttttataa acttgatgca
     2821 ggggaattta gtgagactaa gcggatgatt ttgctgaagt aattttgaaa ttgtctgaac
     2881 cttgatttgc ttgaattgag gatttcttga ttttgagatt agttttggaa tgcgctcaat
     2941 cacagattaa taatctgtgc tacttcacga ttgttaatct cggatgcggc attaaatcat
     3001 gtaatcatga aatcctttaa tcatggttca gacaagcgga agatttaagt aataaaataa
     3061 aataaaattt gaatttaatt ttattttaat ttcaaataat aatccgcttt ctatacattc
     3121 gtaattccta atttttaatt cccaattgaa ttatcccatc gtctttcgaa tgcacagtaa
     3181 actccatatt atctatctca cctgtctgat tatccgcagt ccatttttcc gtgattaact
     3241 tttcatcttt aatatcaaaa tattccaaag tcagttcttt gtcttttata ctcatgatcg
     3301 taaaaccatt gtatccaata tcaaatcctt tgatctttgt ataaaccctt tcgtcatata
     3361 aaataagatt tcttttttta tccatgtcag gaaggatttt gtttttaaat tcaatcggca
     3421 tacctccgtt accaatgcat ctgccatatg cagctattcc attttcggaa gaatatttcc
     3481 caaacatagt cagacggtgc tcatgtcccc atatccagat cacaggtctg tctttaccga
     3541 taagttctgc caactgttcc gctggtttca catattcatc ctctttctca aatgcagtgc
     3601 agtactgatg atgtgtcatg aatattagtc cgcgtttgtc ttcatttatt ttaacttctt
     3661 ctttgagcca cttaagaatt tcttttctga attcactttt cggtttgaaa atatattcga
     3721 gtaatggaat accgactgaa ttataacctg tgtccagaca aatgaccctc cagtgttcat
     3781 tctctataca aaagtaact
//
LOCUS       DAORVQ010000261         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19525_length_3776_cov_4.264983, whole genome shotgun sequence.
ACCESSION   DAORVQ010000261 DAORVQ010000000
VERSION     DAORVQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19525_length_3776_cov_4.264983"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLX80_13770"
     CDS             complement(<1..835)
                     /locus_tag="PLX80_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY21904.1"
                     /translation="MSENLIEVKNLKKYFPIKKGVFSKTIGNVKAVDDVSFTIKKGET
                     LGLVGESGCGKTTVGRTILRLIEPTAGEVFFRGKDITKISEQELKTYRKKMQIIFQDP
                     YSSLNPRITVGGMLNEILKFHEIAEGEAAEKRVEQLLDTVGLRKIHARRYPHEFSGGQ
                     RQRIGIARALSVEPDFIVCDEPVSALDVSIQSQILNLLQDLQAEFGITYLFISHDLSV
                     VEHISDRVCVMYLGQLVEMAKREELYHDPRHPYSQALLSAVPLPDPRNKSKRIILTGD
                     VP"
     gene            1129..1758
                     /locus_tag="PLX80_13775"
     CDS             1129..1758
                     /locus_tag="PLX80_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQY21905.1"
                     /translation="MDLISDFFTNLKDLEGLIRWGGLTVLIIIIFAETGLLVGFFLPG
                     DSLLITAGLIAAQGYLDIYMLNVSLILAAIIGDQVGYWFGKKTGPKIFKREKSLFFAK
                     DHLVKAKHFYEKYGGRAIIYARFVPFARTFAPIVAGVGEMDYKKFISFNIFGGIFWVS
                     SMTLLGYFFGNIPFIKKNFEFVIIGVIILSIVPIIIGYLSHRKETKLKS"
     gene            complement(1771..2802)
                     /locus_tag="PLX80_13780"
     CDS             complement(1771..2802)
                     /locus_tag="PLX80_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQY21906.1"
                     /translation="MKKYLKTIVLFAITFFFLYLAFKGNDFSELFEKLKSANYFYALA
                     GAFTGVVIGSYIRALRWRYFLNPLKKDLPIYNLFSSTMIGYMMNSIIPRTGEIYRPVL
                     LANKEKISRASAFGTILVERVFDVLTMLVSFGICIMYFKQKLSSTFEGYNLEAISLYT
                     FIFLLLFIIVIIVILFNLERSEEIIDKIARKFLPEKFHSEIRKVFVSLINGFLFIKYP
                     KYYLQIFLLTVLLWFSYVCSTYLTFLAFDMDLNLLDANLVLTMITFAVTVPLPANSAG
                     IYHFFCTATLVNIYGINSETAFGYATVSHLLGLLSLILIGAYFFLKENLTFKKAKEFT
                     KSETEPETG"
     gene            complement(2900..3526)
                     /gene="lolA"
                     /locus_tag="PLX80_13785"
     CDS             complement(2900..3526)
                     /gene="lolA"
                     /locus_tag="PLX80_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HQY21907.1"
                     /translation="MNILLKILILIFFISVNFTFAQDAQEIIKKVQSKYENISDAKAS
                     FTETRKGKSGKANSSSGTIYIKKTNKYRIEAGGQIIITDGETSWSYSPKKKQVVIDTY
                     KDDGSTFSPNKFLFNYPENFYSDLQGEENINGVDCYVIKLSPRNSGSVKSATIWIDKK
                     EDFIRKINIVTSESTNTYSLKDITLDSGVNSSKFSFTPPEGVEIIDLR"
BASE COUNT         1267 a          597 c          564 g         1348 t
ORIGIN      
        1 aaggaacatc accagtcaaa ataattctct ttgatttatt tctcggatca ggtaatggaa
       61 cagcagaaag taacgcttgt gaataaggat gccttggatc atgataaagc tcttctcttt
      121 tcgccatctc aacaagctgt cctagataca ttacgcatac cctgtctgaa atgtgttcca
      181 ccactgatag atcatgtgaa ataaacagat aagtaatacc aaactcagcc tgaaggtctt
      241 gaagtaaatt taagatctgt gactgaattg aaacatcaag tgctgaaacc ggttcgtcac
      301 atacaataaa atctggttct accgacaaag cccttgcaat tccaatcctt tgtctctgtc
      361 cgcctgaaaa ctcatgagga tatcttcgtg catgaatttt tctgagacca acagtgtcaa
      421 gtaactgctc aactcttttt tcagcagctt ctccttcagc aatctcatgg aatttcaaaa
      481 tctcatttaa cattccgccg acagtgatgc gggggttcag agaagaataa ggatcctgaa
      541 aaataatctg catttttttt ctgtatgtct taagttcctg ttcactgatc ttagtaatat
      601 cttttccccg gaaaaaaact tcaccggctg ttggctcgat cagtctcaat attgtccttc
      661 ctactgttgt ctttccgcag ccggactctc ctaccaatcc gagtgtctca ccttttttaa
      721 tagtaaatga tacatcatca actgctttta cattaccaat tgtttttgaa aatacacctt
      781 tctttattgg aaagtatttt tttaaattct ttacctcaat aagattttct gacatataaa
      841 ataaatgtta agatattgta atttttagag ataaatataa ttcattgaaa ttgtaaaaac
      901 aatcaagttg aatttctata tttttatttt tggtaaaggc tttttaattt agtatcaaat
      961 tttaataaat acatattgcc ataaaatatt ttaaaatatg ttttcattaa ttattgaaag
     1021 aaagttgaca agcaataatt aagaaaatta tccttatttt tgacactatt cttaattaca
     1081 atatcactaa attgaatgct ttcaaatcaa attatatttt aactattaat ggatttaatt
     1141 tcagattttt ttactaatct caaggacctg gaaggactca taagatgggg cgggttgaca
     1201 gttcttatta ttattatatt tgctgagacc ggattacttg ttggattttt tctgcctggt
     1261 gattcacttt taattacagc gggtttgatt gctgcacagg gatatttgga tatttatatg
     1321 ttaaatgttt cacttattct ggctgctatc attggagatc aggtcggcta ctggtttggt
     1381 aaaaagaccg gtccaaaaat tttcaaaaga gaaaaatcac ttttctttgc aaaagaccat
     1441 ttagtcaaag caaaacattt ttatgaaaaa tacggaggga gagcaattat atatgcgaga
     1501 tttgttccat ttgcaagaac cttcgcaccg atagttgcag gagtaggtga aatggattat
     1561 aaaaaattta tatcatttaa tatttttgga ggaatattct gggtgtcatc aatgacttta
     1621 ctcggttact tttttggtaa tattccattt ataaaaaaga attttgaatt cgtcattatc
     1681 ggagttataa ttttatctat agtacctatt ataattggat atttgagtca cagaaaagaa
     1741 actaaactaa aatcttaaga cagggttaca tcatccggtt tctggttcag tttctgattt
     1801 tgtgaattct tttgcttttt taaaagtgag gttttctttc agaaaaaagt aagctccaat
     1861 cagtatcagg ctaagtagtc caagtaaatg actaacagta gcatatccaa aagcagtctc
     1921 actatttatt ccatatatat tcacaagagt agcagtacag aagaaatgat atatacctgc
     1981 tgagtttgca ggcaaaggaa ctgttactgc aaatgtgatc atagtcaaaa caagatttgc
     2041 atccagcaga ttaagatcca tatcaaatgc cagaaaggtc agatatgtcg aacagacata
     2101 agaaaaccat agtaagactg ttagtaagaa tatttgcaga tagtatttcg ggtatttgat
     2161 aaaaaggaat ccatttataa gagatacaaa tacttttcta atttccgagt gaaatttttc
     2221 agggagaaat ttccttgcta tcttgtctat gatctcttca gatctttcca gattaaaaag
     2281 tatcacaatt ataactatta taaaaagaag taaaaaaata aatgtgtata aagaaattgc
     2341 ttcaagattg taaccttcaa atgttgagga taatttttgt ttgaaataca ttatacagat
     2401 cccgaaagat actaacatag tcaatacatc aaaaactctc tcgacaagta ttgttccaaa
     2461 cgctgatgcc cttgaaattt tttctttatt tgcaagtaat acaggtctgt atatttctcc
     2521 tgttctcggg attatactgt tcatcatata gccgatcatt gttgatgaaa acaggttgta
     2581 aattggtaaa tcctttttca aaggatttaa aaaatatctc catcttaaag ctcttatata
     2641 acttccgatc actacaccgg taaatgctcc ggcaagagca taaaaataat ttgcactttt
     2701 taatttttca aacaactcac taaagtcatt ccctttgaag gcaagataca ggaagaaaaa
     2761 agtaatagca aataagacta ttgtctttaa atattttttc attttgtcgc tcaaatattt
     2821 tactaaatgt aaaattaaaa aagttaagca attattaaat tgcttaactt tatttttaat
     2881 aacttaatac caaaaattgc tatcttagat caattatttc aactccttca ggaggagtga
     2941 aactgaattt agaagaattc acacctgaat ccagtgtaat atcttttaat gaataagtgt
     3001 ttgtggactc ggaagtaaca atatttattt ttctgataaa atcttctttt ttatctatcc
     3061 agattgttgc acttttcaca gaaccactgt ttcttggtga aagcttaatt acatagcagt
     3121 ccacgccgtt aatattttct tcaccctgca gatccgaata gaaattttcg ggataattaa
     3181 ataagaattt attaggtgag aatgtgctac cgtcatcctt ataagtatct ataactactt
     3241 gttttttctt tggagaatat gaccaggaag tctcgccatc tgttataata atctgtccac
     3301 ccgcttctat tctgtactta ttggttttct ttatatatat agtacctgaa gatgagtttg
     3361 ctttaccact ttttcctttt cgggtttcag taaaagaagc ttttgcgtca gaaatatttt
     3421 catattttga ctgtactttc tttataatct cctgagcatc ctgtgcaaaa gtaaaattta
     3481 cagatatgaa aaaaataaga attagtattt ttaataaaat attcattttg gatttaatta
     3541 gttggtaaat tattaatttt aattatggtt atcaatttaa ataattatcc tacttttaga
     3601 attgtatttt taaaatcatt tcggacaaac aggttaatta tattaaaact gatcatttta
     3661 aatccattat aacaaagtta atataatttg ttccggaata atgaaaaatt tatttttaaa
     3721 cagaaagaat taattaatat tttaactaat gccaaccgtt tcttttaaaa cttatc
//
LOCUS       DAORVQ010000262         3775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19531_length_3775_cov_7.326882, whole genome shotgun sequence.
ACCESSION   DAORVQ010000262 DAORVQ010000000
VERSION     DAORVQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19531_length_3775_cov_7.326882"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLX80_13790"
     CDS             <1..337
                     /locus_tag="PLX80_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQY21908.1"
                     /translation="TGIQSESKVNISPNPFSPDGDGHEDFTVINFNLNVKIAQIRVKV
                     YDIKGRIVRTLMDNSISGNNGEIIFNGLDDKEQKLRTGIYILLIEVIDETGGTSDNLK
                     VPLVIATKL"
     gene            complement(350..3352)
                     /locus_tag="PLX80_13795"
     CDS             complement(350..3352)
                     /locus_tag="PLX80_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQY21909.1"
                     /translation="MTGFYFAKFTSFLISIFLISFFTSNLSHSAIYYISNSGNDSAAG
                     TSTVTAWATISKVNSKMSTFIAGDQILFRKGDTFFGQITMTKSGTAGNEIIFGSYGTG
                     QLPIITGAKSPQSWIVHSGNIYKTTFTDTVSNLYLDGKIMNIARYPNTGWLRVDYGTG
                     AGEKGGFKDAALTEGGGYWNGVTCKIRTADWSYEVRTVSGFNSGNVTFSTPTVNRMIY
                     EYGYYLDNRLSLLDTEGEFYYDIPTQVLYFYAPGGVNPNTLSLQATVTRFNIYLNPGV
                     KYVTIQDLHLTNAKEKGVEEANGTSITVKNCRISKTDKIGIRLYGTGHRIENNIIEDV
                     LDIGITAQIFDGFVTGNIINRTGLKPGYGGSSWGYIGLRAESSTGTSIRYNNIDSTGY
                     TGIRFGKNNILEYNIVDYSCVILNDGGGIAIDNIDGALIKNNVVKNSIGNKESCPATN
                     YNLSYGIYFGNTLIKNLLISKNTIAHSRFVGIYIDNTVTLDNIQIVDNVIYNSAHTQI
                     KFSDLSASSYVPTYNTIVKRNIFYSLGWNQVSMEHQMFHSLIFSDYGVFDSNYYCNPY
                     NEFTLKRTIMPPNYSSNDYRLSQWQTVTGEDPNSMYSPFIFEQHYITDTLSSNYITNP
                     HFNDTIAGWTTWPSGSSIARVIHPLLNGGSMRLRWNGIGFTENLAVSNSFPTTKGDYY
                     NVSFSVVGNHTGDFNLWGRSSIPYVYEMGLRRKLRYENYRKDYSVIFKADTTDPNTTL
                     TTGMKLPDSLTYFDNINFYKVNVQKIDSTEQSKIFINETNSTLPFPVNGINYKDLDGT
                     PVGGTILVPPYSSKILINDGFQPVKTLNLTVLLEGLYNTVNQQTSIDTVSVYLRNVNP
                     PYNRIDSAKKVFTASGQISVQFPNAVTGTYYYLEIGHRNSIETWSRAGGETFTGNVLN
                     YNMTNDSTKAYGSNLVKKGTKYCIFSGDSNKDGAIDLADLADIDNNAANFLFGYFSTD
                     INNDNLTDIADLSISDNNAFNIITKVTP"
BASE COUNT         1214 a          651 c          643 g         1267 t
ORIGIN      
        1 gacaggtatt cagagtgaat cgaaagtaaa tatttcccca aatccttttt caccggatgg
       61 ggacgggcat gaagatttta ctgtaataaa ttttaatctg aatgtaaaaa ttgctcagat
      121 cagagttaaa gtttatgata ttaaaggaag gatcgtacga acattaatgg ataattctat
      181 cagcggaaat aatggtgaaa ttatttttaa cggacttgat gataaggaac aaaagttgag
      241 aacaggtatt tatattttac tcattgaagt aatagatgaa acaggcggta cttcagataa
      301 tctgaaagta ccgcttgtaa ttgcaactaa gctttaagag taaattattt taaggagtaa
      361 ctttggttat aatattaaag gcattgttat cggaaatgga aagatctgca atatcagtta
      421 agttatcatt attaatatct gtactaaagt aaccgaataa aaagtttgca gcgttattat
      481 caatatctgc aagatcagca agatctattg ctccgtcttt atttgaatca cctgagaaaa
      541 tgcagtactt ggttcctttt ttgaccagat tggatccgta tgcttttgtt gaatcattag
      601 tcatattata attgagtaca tttccggtaa atgtctcacc acctgctctg ctccatgttt
      661 cgatagaatt tctgtgtccg atctcaagat aatagtaggt acctgttaca gcatttggaa
      721 attgaactga aatctgtcca gaggcagtga aaactttttt tgcagaatca attctgttgt
      781 aaggtggatt aacatttcgt aaatatacag aaactgtatc aatactggtt tgctggttaa
      841 cggtattata caaaccttct aaaagtactg tcagattcag ggtctttacc ggttgaaatc
      901 cgtcattgat cagaatctta gagctgtaag gaggaacaag tattgtacca cctacgggag
      961 ttccgtccag gtctttataa ttgatgccgt ttactggaaa tggtaaagtc gaatttgttt
     1021 cattgataaa tatttttgac tgctcagtag agtcaatttt ctgaacatta actttataaa
     1081 aattaatatt atcaaaataa gtcagtgaat caggaagttt cattccggtt gtgagagtag
     1141 tatttggatc tgtagtatca gctttaaaaa taactgaata atcttttctg taattttcgt
     1201 acctcagttt tcttcttaaa cccatttcat aaacatatgg aatacttgat ctgccccaga
     1261 gattaaagtc accggtatga tttcctacca cactgaaact tacattataa taatctcctt
     1321 ttgtagtagg gaatgaatta cttactgcga gattttctgt gaatccgatg ccgttccatc
     1381 gaagtctcat cgagcctccg ttaagaagtg gatggatgac tcttgcaatg gaagaaccgg
     1441 aaggccatgt tgtccagccg gcaattgtat cattgaaatg tggattagta atatagttac
     1501 tgcttaatgt atcagtgata taatgttgtt caaaaataaa cggactatac attgaatttg
     1561 gatcttcacc tgtaacagtc tgccattgag ataatctgta atcgttgctg gaataattcg
     1621 gcggcataat agtccttttc aaagtaaatt cattataagg attacaataa tagttactgt
     1681 caaaaactcc ataatcactg aagatcaatg agtgaaacat ctgatgttcc atagagactt
     1741 gattccatcc caggctataa aaaatatttc ttttaacaat ggtattgtaa gtcggaacat
     1801 aggaagatgc agagagatca gaaaatttta tttgtgtatg tgcactattg taaattacat
     1861 tatctacaat ttgaatattg tccagagtta cggtattatc aatataaatc ccgacaaatc
     1921 tgctgtgagc aatagtattt tttgagatta aaagattttt aataagtgta tttccgaaat
     1981 agattccgta gctaagattg taatttgtag ccggacagga ttctttattt ccgatggaat
     2041 tttttacaac attatttttt atcaatgctc cgtcgatatt gtcaattgca atcccacctc
     2101 cgtcatttaa aatgacacag gagtaatcta caatattata ttctaaaata ttattttttc
     2161 caaaacgaat tcccgtatat cctgtactgt caatattgtt atatcttata gaagtacctg
     2221 tagaactttc tgctcttaat ccaatataac cccatgaact gcctccatac ccgggtttta
     2281 atcctgtccg gtttataata tttccggtta caaagccgtc aaatatctga gctgtaatac
     2341 cgatatcaag aacatcttca ataatattat tttctattct atgacctgtt ccataaagtc
     2401 tgatcccgat cttatcagtt ttactgatcc tgcaattttt tacagtaatg gaagttccat
     2461 ttgcttcttc aactcctttt tcttttgcat tggttaaatg aagatcctgt atggtcacat
     2521 attttactcc cggattcaga tatatattaa atcttgtcac agttgcctgc aatgaaagtg
     2581 tatttggatt taccccaccc ggcgcataaa agtataatac ttgtgtcgga atgtcataat
     2641 aaaattcacc ttcagtatcc agaagactta gtctgttatc taaataataa ccgtattcat
     2701 atatcatccg gtttacagta ggagttgaga aagtgacatt tccactgttg aatcctgaga
     2761 cagttctgac ttcatatgac cagtcagcag ttcggatttt acaggttaca ccattccagt
     2821 atccgcctcc ttctgtaagt gctgcatctt tgaatccgcc tttctcaccg gcaccggttc
     2881 cgtaatcaac tctgagccag cctgtatttg gataccttgc aatgttcatg atctttccgt
     2941 ctaagtatag atttgataca gtatcggtaa aagttgtttt ataaatatta cctgaatgaa
     3001 ctatccagct ttgtggtgac ttagctccag taatgatagg aagctggccg gttccataac
     3061 tcccgaaaat aatttcatta ccggcagttc cggattttgt cattgtgatc tgtccaaaaa
     3121 aggtatcacc ctttctgaat aatatctgat cacctgcaat aaaagtactc atcttggaat
     3181 ttaccttact gattgtagcc caggcagtta ccgtgctggt tccggctgca ctatcatttc
     3241 cactgtttga aatataataa atagcagaat gagataagtt agatgtaaaa aacgaaataa
     3301 gaaaaatgga aatcagaaaa gaagtgaatt ttgcgaaata aaaacccgtc atattaaatt
     3361 tataatttta aatttgaaat acttggggta tgcaaaccta attgaaacta ctttctaatt
     3421 caagataaat ttatttcaat aaaaaattat ctctgtacac gacaaaactt ttgaataaaa
     3481 aataaaaatc tgatccaaaa ttgttatgtt tagataattc actatttaaa aataaaattg
     3541 aaaggatcag attatggaac atattaacaa aatcagagaa attttacaac cgtattttga
     3601 ttgcgattcc aggagattgg attttatttc taaatttgca gcaagtttaa tacgtgctcg
     3661 tacggtagta ttaagttacc cgtctctcaa aacgaattgc ctgtctatca aaacgaattg
     3721 cccgtctctc aaaacgaatt gcccgcccct caaaacgaat tgcctgtcta tcaaa
//
LOCUS       DAORVQ010000263         3773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19542_length_3773_cov_7.115385, whole genome shotgun sequence.
ACCESSION   DAORVQ010000263 DAORVQ010000000
VERSION     DAORVQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19542_length_3773_cov_7.115385"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..>3773
                     /locus_tag="PLX80_13800"
     CDS             458..>3773
                     /locus_tag="PLX80_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21910.1"
                     /translation="MKTSVQKLFLSFLMIFFVTGTSFSANEYFRSITSGDWNSLSTWE
                     MSVNNFTWVPATSTPDETSNLITIRTPNTVTVTVSVSADQLTVNNGGTLSIDSKILLT
                     ILDGSGTDLTVTTGGTVSGAGTVQTQGADVTVNINTNASFSADLLVNSGNTMLLDPGG
                     PYTPSITGDITINNSAALQGGSGGYFIIAKGNVLNNGTIEGGSGSSFVMRGASLVNNG
                     AITINNLRFDSTTALSGTGTYTSNAIAITAAGNVSLSNNITFAPTSSFTINAGGVLDP
                     TNIFTFENGTFSLSNGGTVLNSGTFRTRDTVTVLVRPVSNFRAPFNVSDGITTLYDQS
                     GPFNAPMYGTITIDAGAVLAGGPGGYTITAYDNVINEGSIIGGSSTSFTMKGASFLNN
                     GLITITNLNFDSTTAVSGSGVFSSTNTYINSTGIVSLSNDITFTPVTTFTVNSGGILD
                     PNGNIFTLSTAKFLLNPGGTVANSGTFRTQDTVIVNIRNGSSFNCALNINSGLTTLYD
                     PSGPFISTLFGTLTVDAGAALAGGPGGYYIFAMNTVTNNGTIQGSSSTSFVMKGPDMI
                     NNGLVSIPTMRFDSTTTLSGTGTYTSTAMIINPTGNVSLTSDMNFAPSSSLTVNNGGI
                     LNLNTYILTFSSATLLVNNGATIVNSGLLKTTGAVTLDIRNTANFNVPLNIESGTNTT
                     YSAAGPFVSSLNGPVTVDSGAVWQGASGGYTININNDITNNGTITGNVSEMNFIGTNL
                     INNGSILVNVFNFESGNHTLQGTGSWGTLGRFLSGSTTALLSTHQMRDININSGGTFD
                     ISSQKLLLSGSGNPVTQNGTFTTTGSTIEYNGTAGQTAATASVDYHSLTVNNAAGVDL
                     PAAETVPATLTLTQGTFTTGAFLTLGNGATIVRQDGTLSAVPTFGANVNVIYIGTNAT
                     NSSVEIPAATTVLRNLTIDNTGGVTLTAATTVNDTIFLTSGVFSPLGNLTMADPITIN
                     RATGTFDNGQVFPANVNVVYSGTTAVNTDFELPATATELQNLTVNNPGGVTLFSPITV
                     NDLLSIIKGDLDLNGNIITMGSAANMTETDSNTVKGSTGYIVAVRDLNAPSSENVAGM
                     GAVITSAVNMGSTEIRR"
BASE COUNT         1238 a          728 c          710 g         1097 t
ORIGIN      
        1 attaactatt aactattaac tattaactat taactattaa ctatctccag taatttgctt
       61 cttttttcta aaaatgaaca ggcagataat taaaataatg atgtttaaaa gctaaaactc
      121 aactttcctt gtaaaaatat taaacatatc taataaaaac atcctaaacc tggaaaaata
      181 tttctttgtg aaatcaaatt aaataaaata tatttgtatc aaggcgatcg gtttgaatta
      241 gatttaaaat cccactaatt tttctacacc aaaatccgct gtaacggaca aactctttcg
      301 gcttaatcga tgagacaatt ttcagtatta ttttgatcta tagatcaaat acatggaaat
      361 taaagagtta aaaattatat atattattta taaaaaatat ctatatataa aaaatcaaat
      421 acaaatattt aaaaataacc taagaaaggg gaaataaatg aaaacctcag tacaaaagct
      481 gttcttatct tttttgatga tcttttttgt aaccggaaca tcattttcag ctaatgaata
      541 tttcagaagc ataacttcag gcgactggaa ttcactctcg acatgggaaa tgtcagtaaa
      601 caattttaca tgggtaccgg cgacttccac tccggatgaa acaagtaatt taataactat
      661 cagaactcca aatactgtca cggttacagt aagtgtaagc gccgaccaat tgacagtaaa
      721 taacggagga actctttcca ttgattcgaa aatattgctc accattcttg acggtagcgg
      781 aacagatctc acagtaacta caggtggaac agtatcaggg gcaggaactg tgcagactca
      841 gggtgcagat gtgacagtca atattaacac caatgcaagt ttcagcgctg atctattggt
      901 aaattcagga aatacaatgc ttttggatcc cggtggtcct tatactcctt caatcacagg
      961 agacattacg atcaataata gtgctgcttt acagggaggc agcggaggat attttataat
     1021 cgcaaaaggt aatgtattaa ataacggaac tattgaaggt ggaagcggat cttcttttgt
     1081 aatgagagga gcttcgcttg taaataatgg tgcgataacg ataaataatc ttagatttga
     1141 ttcgactact gccctgtccg gtacaggaac ttatacaagt aatgcaatag ccataactgc
     1201 tgcaggaaat gtttcacttt caaataatat aacctttgca ccaacctctt catttacaat
     1261 taatgcagga ggagttcttg atccgacaaa tatcttcaca tttgaaaacg gtacattttc
     1321 attgagtaac ggcggtactg ttttaaactc cggtacattc cgtaccaggg atacagttac
     1381 agttctggtc agaccggttt ctaatttccg ggcaccgttc aatgtaagtg atggtattac
     1441 tactctctat gatcaaagcg gtccgtttaa tgcaccgatg tacggaacaa taaccattga
     1501 tgcaggagca gttttggcag gaggtccggg aggatataca attactgctt atgacaatgt
     1561 aataaatgaa ggatcaatta tcggcggctc atcaacaagt ttcacaatga aaggagcttc
     1621 atttttaaac aatggattga tcaccataac taatttaaat tttgattcca ctactgctgt
     1681 gtcgggttct ggtgtcttct caagtactaa tacatatata aacagtacag gtattgtttc
     1741 actttccaat gatattacat ttacacccgt tactactttt acggttaatt ccggaggaat
     1801 tttggatcca aacggaaata tatttacact ttctacagca aaattcctgc tgaatccggg
     1861 aggaacagtt gcaaactcag gaactttcag aacgcaggat acagtaattg tcaatatcag
     1921 aaacggatct agctttaatt gtgcgttaaa tattaattca ggacttacca cactatatga
     1981 tccgagtggt ccctttattt ctacactttt cggaactctc actgttgatg caggagcggc
     2041 tttggctgga ggacccggcg gatattacat atttgccatg aatacagtga caaataatgg
     2101 aacaatccag ggctcaagtt caacatcatt tgtaatgaaa ggtcctgata tgataaataa
     2161 cggtttagtt tcaattccaa caatgcgttt tgattctacg actactttgt caggaacagg
     2221 gacttatacc agtaccgcaa tgatcattaa tccaacagga aatgtatctt taaccagcga
     2281 catgaatttc gcaccgtcat catcacttac tgttaataac ggaggaattt taaatcttaa
     2341 tacatatatt ttaacttttt cttcggcaac cttattggta aataacggag ctacaattgt
     2401 aaattcggga ctgcttaaga caaccggagc agtaacactg gatatcagga atactgctaa
     2461 ctttaatgtt cccctgaata ttgaatccgg taccaatact acatattctg ctgcaggtcc
     2521 gtttgtttca tctctgaacg gtcctgtaac agttgactcc ggggccgtat ggcagggagc
     2581 cagcggcggt tatactataa acattaataa tgatattaca aacaacggaa ctattacagg
     2641 gaatgtatct gaaatgaatt ttatcggtac aaatctgatc aacaacggaa gcattcttgt
     2701 gaatgtgttt aattttgaat ccggaaatca cactcttcag ggaacaggtt catggggaac
     2761 tttaggacga ttcctttcag gttcgactac ggctttatta agtacacatc agatgcgtga
     2821 cataaatatt aactcaggcg gaacatttga catttcttca caaaaattat tactatccgg
     2881 aagcggcaat ccggtcacac aaaacggaac tttcacaact acaggcagca caatagaata
     2941 taacggaact gccggacaga ctgcagcgac tgcctcagtt gattatcaca gtctaaccgt
     3001 taataatgca gccggagttg atcttccagc tgcagaaact gttcccgcta cccttacttt
     3061 aacacaggga actttcacaa caggtgcatt tctgacttta ggaaacggcg caacgatcgt
     3121 aagacaggac ggaacattgt ctgctgtacc gactttcgga gcaaacgtga atgtaattta
     3181 tattggtacc aatgctacaa actcatctgt agaaatacct gcagctacta ctgttctgag
     3241 aaatcttaca atagataata caggcggtgt tacattaact gctgcaacaa cagtaaatga
     3301 tacgatcttc cttacaagcg gagttttctc acctttaggt aatttaacta tggcagatcc
     3361 gatcaccatt aacagagcaa caggaacatt cgacaatggc caggtttttc cggctaacgt
     3421 gaatgtagtt tattcgggaa cgactgccgt aaatacagat tttgaacttc cggctactgc
     3481 aacagagctg caaaatctga ctgtaaataa tccgggtgga gttactctct tttcaccaat
     3541 tactgtcaat gatctgctaa gtattataaa aggagatctg gatcttaacg gaaacataat
     3601 tacaatgggc tcggctgcaa atatgacgga aactgacagc aacacagtaa agggttcgac
     3661 aggatatatc gtcgcagtca gagacctcaa tgctccgtca tcagaaaatg ttgccggcat
     3721 gggagcggtc ataacatcag ctgtaaatat gggttcgact gagatcagaa gag
//
LOCUS       DAORVQ010000264         3758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19669_length_3758_cov_6.209020, whole genome shotgun sequence.
ACCESSION   DAORVQ010000264 DAORVQ010000000
VERSION     DAORVQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19669_length_3758_cov_6.209020"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..639
                     /locus_tag="PLX80_13805"
     CDS             58..639
                     /locus_tag="PLX80_13805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY21911.1"
                     /translation="MSDFKVHPTAIVDDNVEIGSGTSIWHFSHVQPGAKIGEKCTIGQ
                     NVNIANNVTIGNFCKIQNNVSVYEGVTLEDYVFCGPSMVFTNIILPKCKYPQRGAEFY
                     APTLVKQGASIGANATIVCGTTIGRDCLIGAGTVVTKDVPDYALVVGNPGKVIGWVSE
                     AGKRLEFGLDGLAECEKSGKKYKLEEGKVSEVN"
     gene            1224..1805
                     /locus_tag="PLX80_13810"
     CDS             1224..1805
                     /locus_tag="PLX80_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21912.1"
                     /translation="MKIISYGDIIWTKTYGRNNSQGTTVVSTNDGGYILSGNSGEAFT
                     IKIDRDGNTLWEKYYGVFGIQIKKITKTTEDGFIMCGLENLQNGFILKIDSNGNFVWN
                     KIHKSNDLKIYFDIITDFDKGYLVCGFEVKSQQDTAKAVVFKINDLGNIIWEKTIFYD
                     DQIAAYSIKKSHDNFIIAGSIGTIPITTGRVFL"
     gene            1802..2452
                     /locus_tag="PLX80_13815"
     CDS             1802..2452
                     /locus_tag="PLX80_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21913.1"
                     /translation="MKIDDSGNYKKPVILDTKSNVYFGDFNILGSSKYVFTMTIDSVN
                     NIMPGKLIITDTLGNVIYQKVFPTSDLIEFKSIQMIENGDLIFAGLAGFYIPGSDLVG
                     YVVRTDSLLNYKTTSIQNNNLLITDKFELYQNFPNPFNSSTKIKYKINKESNLLINLI
                     DINGRIITIQKPTKQKPGFYEITLNYEYYNLSSGIYYIEFETDLSFKKVIKTLFIK"
     gene            2557..>3758
                     /locus_tag="PLX80_13820"
     CDS             2557..>3758
                     /locus_tag="PLX80_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21914.1"
                     /translation="MNKFKKIVFVSLFFYLFSTSIFGQTITWQKILNNNYGSFARAYQ
                     TGDNNFIAVGSDVSGVGYKIYLAKYSFNGDSIWTQIIGNNNFSYDGYWIIDDSKKGFI
                     ICGSTNKGGSADGYLLNTDSKGNVIWSRSFNGLGLDQSKCVKKAVDNGYIVLNRTVAN
                     FNNIMLIKTNELGDVEWQKIYHANNDQYGEEVIVLEDSYVIVGSVYADVYLLKVDLNG
                     DTLWSKRFGSIYSEAGYSLQQSKDNGFIIGGVSYNSNNMSNSLIVKTDSAGNLQWQRS
                     YTAIHNELLYSVRNLPGTGYVFCGTTDSLEGNLERGFIRIIDLDGNTLNEKFYRALTY
                     FTEIRSVETTRDNGLILSGVTQELSGGQPKMYLAKTDSVGNIYPVGITNYSSQIPKQF
                     YLNQNYPNP"
BASE COUNT         1364 a          463 c          694 g         1237 t
ORIGIN      
        1 tattaactat taactattaa ctattaacta ttaactatta actattaact ataaaagatg
       61 tctgatttca aagtacaccc aacagcaata gtagacgata atgtagagat cggaagcggc
      121 acatcgatat ggcatttctc acatgtacag cccggcgcaa agatcggcga gaaatgtacg
      181 atcggtcaga atgtaaacat cgctaacaat gtgacgatcg gaaatttttg taagattcag
      241 aataacgttt cagtatatga aggagtgact cttgaagatt atgtcttctg cggaccttcg
      301 atggtattca cgaatataat tttaccaaaa tgtaaatatc cgcagagagg tgcggagttc
      361 tatgcgccga cacttgtaaa gcagggagca tctatcggag ctaatgcgac aatagtatgc
      421 ggcacaacaa taggtcggga ctgtctaatt ggtgcaggta cagtcgttac taaggatgtt
      481 ccggattatg cgcttgtagt cggtaatccc ggcaaagtca tcggctgggt aagtgaagcg
      541 ggaaaacgtc tcgaatttgg tttggatgga ttggcggaat gtgaaaagtc agggaagaaa
      601 tataaacttg aggaagggaa agtaagcgag gtaaattaat ttcatacttt ggcggatata
      661 taacaaacgg attcgtgact tcgtgagata ttacatgata gagttcgatt tgaaacggag
      721 tttcagttac aattgcgttc ccatactgag ttttggaatg agtgggttgc cgttgttggt
      781 cttaagagag cgcagtgaac gtgaccaaca acaaacggaa acgtgacttc ggaagctata
      841 ggttgttggt gatcaaaaag attgtttgag tagaacagga gattgtaatt ttatagcaag
      901 ctatttggaa ctcaagcaaa ggcagtaatt ttgaaattta aaaaaataat agtattcgat
      961 gtaattgtta gattcaatga aataaaattt gaaaaaatag aaatgccatt atgaaactta
     1021 aaatatttat ctgcttatcg gttttgttta tttgctttga gggagtatag gcgcagtcta
     1081 taacatggga aaaaagatac ataaattcta atgctccatt tagaaacggg ataaatgcaa
     1141 tatgcaattc tgattcagga aattattttc ttgttggtta tactttgttt ccttcacaaa
     1201 tcagttatta taaaatatgg gttttgaaaa taatttcata tggggatatt atatggacaa
     1261 agacctatgg aagaaataac agccaaggga caacagttgt ttctactaat gacggtggct
     1321 acattttatc tggaaattct ggcgaagcat ttacaataaa gatagacaga gacggaaata
     1381 cattatggga aaaatattat ggagtatttg gaatacaaat aaaaaaaatt acaaaaacaa
     1441 ctgaagacgg atttataatg tgcggtttgg aaaacttaca aaatggtttt attctaaaaa
     1501 tagattccaa tggaaatttc gtatggaata aaattcataa atctaatgat ttaaaaattt
     1561 attttgatat aattactgat tttgacaagg gatatttagt ttgtggtttt gaagttaaat
     1621 ctcagcagga tacggctaaa gctgtagttt ttaaaattaa tgatttaggc aatataatat
     1681 gggaaaaaac aatattttat gatgaccaga tagctgcata ttcgataaaa aaatctcatg
     1741 ataattttat aatagcaggt tctattggaa ctattcctat aactactgga agagtttttt
     1801 tatgaaaata gatgattcag gaaattataa aaaacctgtg attttagata ctaaaagtaa
     1861 tgtatatttt ggagatttca atattcttgg atcaagcaag tatgtattta caatgacaat
     1921 agattctgta aataatatta tgccgggaaa attaataatt actgatactt tgggtaatgt
     1981 tatttaccaa aaagttttcc ctacttcaga tttaattgaa tttaaatcaa ttcaaatgat
     2041 tgaaaatggt gacttgatat ttgcaggtct tgcaggtttc tatatacctg gttctgatct
     2101 tgttggttat gttgttagaa ctgatagtct gttaaattat aaaactactt cgattcagaa
     2161 taacaattta ttaattacag ataaatttga attataccaa aatttcccaa atccattcaa
     2221 ttcttcaact aaaattaaat ataagataaa caaagaatca aatcttttaa ttaacctgat
     2281 agatattaat ggtagaataa ttactattca aaaaccaacg aaacaaaaac ctggatttta
     2341 tgaaattact ttaaactatg aatattataa tctttcttca ggtatatatt atatcgaatt
     2401 tgaaacagat ttatctttta aaaaagtaat taaaacttta tttattaagt aatgaaaaaa
     2461 aataacatta taactattaa cattcttact tttttaattt ttataataat ttattgtcaa
     2521 aatttatttt tacaatattg taattcaaaa taaatcatga ataaatttaa aaaaatagtt
     2581 tttgtaagtt tgttttttta tttgttttca acttcaatat ttggacaaac aataacttgg
     2641 cagaaaattt taaataataa ttacggaagc tttgcacggg cttatcagac aggtgataat
     2701 aattttattg ctgttggaag tgacgtaagt ggagttggtt ataaaatata tcttgctaaa
     2761 tattctttta atggtgactc aatatggact caaataattg gtaataacaa ttttagttac
     2821 gacggatatt ggataataga tgatagtaaa aagggattca taatatgcgg atctacaaat
     2881 aaaggaggtt cagcagatgg atatctgctt aatacagatt caaaaggaaa tgtcatttgg
     2941 agcagatcat ttaatggatt gggattagac cagtcaaaat gtgttaaaaa agctgtagat
     3001 aatggttata tagttttaaa cagaacagtc gcgaatttca ataatattat gttaatcaag
     3061 acaaacgaac ttggtgatgt agagtggcaa aaaatatatc atgcaaataa tgatcaatat
     3121 ggagaagaag ttattgttct tgaagattca tatgtaatcg taggatcagt ttatgcagat
     3181 gtatatttac ttaaagttga tttaaatgga gatactttat ggagcaaaag atttggatct
     3241 atttattcag aagccggata ttctcttcag cagtcaaaag ataatggatt tattattgga
     3301 ggtgtttcat ataactctaa caatatgtca aattcattaa ttgttaaaac agattcagct
     3361 ggaaatttac aatggcagag atcttatact gcaattcata atgaattact atattcggtt
     3421 aggaatcttc ccggaaccgg ttatgtattt tgcggaacga cagactctct tgagggtaat
     3481 cttgagcggg gttttatcag aataatagat ttggatggaa atacattgaa tgaaaaattt
     3541 tatcgtgctc taacgtattt tactgagatt cgttcagttg aaactaccag agacaatggt
     3601 ttaatattaa gcggagttac acaagagcta tcaggtggtc aaccaaaaat gtatttagca
     3661 aaaactgatt cagttgggaa tatttatccg gttgggatta caaattacag ttctcaaatc
     3721 cctaagcagt tttatttaaa tcagaattac ccaaatcc
//
LOCUS       DAORVQ010000265         3744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_19813_length_3744_cov_4.755489, whole genome shotgun sequence.
ACCESSION   DAORVQ010000265 DAORVQ010000000
VERSION     DAORVQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19813_length_3744_cov_4.755489"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLX80_13825"
     CDS             complement(<1..222)
                     /locus_tag="PLX80_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21915.1"
                     /translation="MNISANTKDIIDKINESSDRFIKNNYEISILIEISFFSDKLKDF
                     RNLIFTSKYVNGLTKVLSSTEITGKEFIER"
     gene            complement(244..3315)
                     /gene="secA"
                     /locus_tag="PLX80_13830"
     CDS             complement(244..3315)
                     /gene="secA"
                     /locus_tag="PLX80_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQY21916.1"
                     /translation="MLSIFKKIFPSKHEKDINSISPVVDEINVFYEEFESLSDEQLKE
                     KTNEFKQKIISETEEINNKISELQKGLTDDLTHNERMNIHDEIDLLKKENYDVINAVL
                     DEILPEAFAVVKQTCKRLCGTEWEAAGNKIKWNMVPYDVQLIGGVVLHQGKIAEMATG
                     EGKTLVATLPLYLNALAGKGVHLITVNDYLAKRDSEWMGEIFKFLGLTIGVILNDMDP
                     NKRREMYNCDITYGTNNEFGFDYLRDNMVVDKEFLVQREHFYAIVDEVDSVLIDEART
                     PLIISGPVEISKHKYDEINPKIKRLVEAQKNLVNKLTAEAEAIVSQENVSSEDSAKAG
                     LALLRANHALPNHKKLQKLLSEPSNKKLLLASEAEYMRDNSKQMHIVDDELYFIIEEK
                     SHVTDLTEKGREFLAPSAEERDFFTLPDFGSEIASIENNTELSIEEKELKKDELSKIY
                     AERSDMLHTVHQLLKAYGLYDKDVDYVVQDSKVMIVDEFTGRILSGRRYSDGLHQAIE
                     AKEGVHVEKDSQTLATITLQNYFRLYKKLAGMTGTAETESSEFFDIYKLDVTVIPTNR
                     DCVRDDLNDLIYRTKREKYNAVIDEIAEMRTQRRPVLVGTTTVEVSETISRLLKRKNI
                     PHEVLNAKQHAKEAHIVENAGLPGAVTIATNMAGRGTDIKLGPGVADSGGLHIIGTER
                     HESRRIDRQLRGRAGRQGDPGASKFYLSLEDELMRLFGNERIANVMERLGFKEGESIE
                     HKMITNSVERAQKKVEENNYAIRKRLLEYDNVMNQQREVIYSKRRQALTGERLKDEIF
                     DMVDISVENTIKKYYEDGNIDGLIEDIQRTYLVRVNISPDDFQKLGESGLKDMILNEA
                     KAYYNRKEERYGSELMGKIERFATLSIIDEKWKEHLREMDDLKEGISFRAYAQKDPLL
                     EYKSDGFKIFVEMLDSLSDEIIKFIFKFTSRAPEEAQNVKAPAPTNQSRLRTYHESAA
                     GMGYVGSDNISGGEESNTKHQPVLVGDKIGRNDPCHCGSGKKFKNCHGRQS"
     gene            3546..>3744
                     /locus_tag="PLX80_13835"
     CDS             3546..>3744
                     /locus_tag="PLX80_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQY21917.1"
                     /translation="MSIMITTECINCGACEPECPNTAIYEGGVDWVLSGNTHGENDTA
                     PSGAEGFYSSEFFYIVPDKCTE"
BASE COUNT         1084 a          737 c          549 g         1374 t
ORIGIN      
        1 tctttcaatg aattccttac ctgtgatctc tgtcgaagat aacacttttg tcaatccatt
       61 aacatatttt gatgtaaaga taagatttcg gaaatctttg agtttgtctg aaaaaaaaga
      121 gatctcaatc aaaattgaga tctcataatt atttttgata aagcggtcag aactttcgtt
      181 tattttatct attatatctt tagtatttgc ggagatattc atatagtaaa attgataaac
      241 ttgttagctt tgacgaccgt gacaattttt aaatttcttt ccgctgccgc aatgacacgg
      301 gtcattcctg ccgattttat ctccgactaa gaccggttga tgcttagtat tggattcttc
      361 accaccactg atattatcag atccgacata gcccattcct gctgcagatt catgataagt
      421 ccttagtctt gattgatttg tcggagccgg tgcttttaca ttctgagctt cttcaggagc
      481 tcgtgatgta aatttaaaaa tgaatttaat aatttcatcc gagagtgaat ccagcatttc
      541 cacaaaaatt ttaaatccgt cagatttata ttccagcaaa ggatctttct gagcataagc
      601 cctgaaacta ataccttctt tcaggtcatc catttctctc aaatgctctt tccatttttc
      661 atcaataata cttaatgttg caaatcgttc tatctttccc atcagctctg atccgtatct
      721 ttcttctttt ctgttataat atgcttttgc ttcatttaag atcatatctt taagaccaga
      781 ttctccaagc ttttgaaaat catcaggtga aatatttact cttacaagat atgtccgttg
      841 aatatcttca ataagtccgt caatgtttcc atcttcgtaa tattttttaa ttgtattctc
      901 cactgaaata tcaaccatat caaagatctc gtctttaagt ctttcaccag tcaaagcttg
      961 tcttcttttg gaataaataa cttctctttg ctggttcatc acattatcat attccaaaag
     1021 tctttttctt atagcgtaat tattttcttc aacttttttc tgagctctct caactgaatt
     1081 agtgatcatt ttatgttcga tcgattcacc ttctttaaag ccaagacgtt ccataacatt
     1141 tgcaattctt tcattaccaa aaagcctcat tagttcatcc tcaagtgaca gataaaactt
     1201 tgatgctcca ggatcaccct gtctgcctgc ccgtcctctc agctgtctgt caattcttcg
     1261 cgactcatgt ctttcagtac caataatatg taacccgcct gaatcggcta cacccggtcc
     1321 cagcttaatg tcagtaccac gaccagccat attagtagca atagtaactg cacccggaag
     1381 acccgcgttt tctacaatat gtgcttcctt agcatgctgc tttgcattaa gtacttcgtg
     1441 aggaatattc tttcttttca gtaatcttga aatagtttca gaaacttcaa cagttgtcgt
     1501 tccaaccagc acaggtcttc tttgagttct catttccgca atctcatcta ttacagcatt
     1561 gtatttctca cgtttggtcc tgtaaataag atcattcaga tcatctctta cacaatctct
     1621 gttggttgga attacagtta catcaagttt gtaaatatca aaaaactctg acgactcagt
     1681 ctcagcagta ccggtcatac cggctaattt tttataaagt ctgaaataat tctgtaatgt
     1741 aatagtcgca agtgtctgcg aatctttttc aacatgtaca ccttcctttg cttctattgc
     1801 ctgatgaagt ccgtctgaat aacgtctacc tgacagaatt cttcctgtga attcatccac
     1861 gatcataact ttactgtctt gtactacata atctacatcc ttatcataca atccataagc
     1921 ttttaaaagc tgatgaacag tatgcagcat atcacttctc tcagcataaa ttttacttag
     1981 ttcatctttt tttaattctt tttcttcaat ggacagttct gtattatttt ctatactagc
     2041 aatctcagat ccgaaatcag gaagtgtaaa gaaatctctt tcttcagcac tgggagcaag
     2101 gaactctctt cctttttcag tcagatcagt tacatgtgat ttttcttcta taataaaata
     2161 caattcatca tcaactatat gcatctgctt cgaattatct ctcatatatt cggcttctga
     2221 agcaagaagt aattttttat ttgaaggctc acttagaagt ttttgaagtt tcttgtgatt
     2281 tggaagagca tgattagctc ttaataatgc aagtccggct ttggcactgt cttcagaaga
     2341 aacattttcc tgtgaaacta ttgcttcagc ttctgctgtc agtttattta caagattttt
     2401 ctgagcttca acaagacgtt ttattttggg attaatttca tcatatttat gctttgatat
     2461 ttccaccggc cctgagataa tcagcggtgt ccttgcttca tcaataagta cagaatccac
     2521 ttcatcaacg atagcataaa aatgttcccg ctgcacaaga aattctttat ccactaccat
     2581 attgtctctg agataatcaa aaccaaattc attattcgta ccgtaagtaa tatcacagtt
     2641 atacatttca cgccttttgt tgggatccat atcattcaga atcacaccaa tagtaagacc
     2701 aagaaattta aatatttcac ccatccattc actgtctctt tttgcaaggt aatcatttac
     2761 agtgatcaga tgaacgcctt ttccggcgag tgcattgaga tacaaaggaa gagtagctac
     2821 aagagtcttt ccttcacctg tcgccatttc agcaatctta ccctgatgaa gcaccactcc
     2881 accgatcaac tgaacatcat aaggtaccat attccatttg atcttattac cggcagcttc
     2941 ccattccgta ccgcatagtc ttttacatgt ctgctttact actgcaaaag cttctggcaa
     3001 aatttcatca agaacagcat taattacatc gtaattttcc tttttaagaa gatcgatctc
     3061 gtcatgaata ttcattcttt cattgtgagt aagatcgtct gtaagtcctt tttgcagttc
     3121 ggatatttta ttatttattt cttcagtttc agaaatgatt ttctgcttga attcatttgt
     3181 cttttctttt agctgttcgt cggaaagtga ttcaaactct tcataaaata cattgatttc
     3241 atcgacaacc ggtgaaattg aatttatatc tttttcgtgt ttagatggaa atattttttt
     3301 aaatatgctt aacatttaat ttgattattt atattaattt tttcttaaca atcaatttac
     3361 aatttttaat atcgaaatga aaaataaaaa ataatgctaa aaaggtcttc agtttagttg
     3421 gaatttattg aaaagaatct taataaatat ttattctaaa tatcctattc aataaattta
     3481 aaattgaata agaaagttta tttaattaat ttgtttaaaa ttaaattact taggaggttt
     3541 ttttaatgtc gataatgata acaacagaat gtatcaactg tggtgcatgt gagcctgaat
     3601 gtccgaatac tgctatttat gaaggtggtg tggattgggt tttaagtggt aatacgcatg
     3661 gagagaatga tactgctccg tcaggtgcag aaggatttta ttcaagtgaa tttttttata
     3721 tagttcctga taaatgtact gagt
//
LOCUS       DAORVQ010000266         3723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20018_length_3723_cov_4.903762, whole genome shotgun sequence.
ACCESSION   DAORVQ010000266 DAORVQ010000000
VERSION     DAORVQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20018_length_3723_cov_4.903762"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..894
                     /locus_tag="PLX80_13840"
     CDS             160..894
                     /locus_tag="PLX80_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21918.1"
                     /translation="MIEIKNLKKSFGNKEVLRGVNLTIEDGITLVIIGRSGCGKSVLL
                     KHIIGLLKPDEGEVLIEGNDIAKMDQKELYEIRKRFGFLFQGAALFDSMDVEENIGLA
                     LKENTSMTKSEIADVVVEKLELVGLPGTQNMKPADLSGGMKKRVSLARSLATNPEYIL
                     YDEPTTGLDPVMSDQIDDLIQDLSKKLKVTSIVVTHDIFSVYDIADVVAMMHEGRIYF
                     IGTPKELTTTDDKLIRDFLNRTDKRI"
     gene            complement(1058..1423)
                     /locus_tag="PLX80_13845"
     CDS             complement(1058..1423)
                     /locus_tag="PLX80_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY21919.1"
                     /translation="MKKTFHELCEIIKKNITECTVKDVMEMKKNNEDHILIDIREDNE
                     FEKGRIKGAVHLGKGIIERDIHLFVNDHDKKIVLYCGGGYRSAIAAESLQKMGYTNVH
                     SMDGGWKGWNAEEGEVELP"
     gene            complement(1435..2409)
                     /gene="pta"
                     /locus_tag="PLX80_13850"
     CDS             complement(1435..2409)
                     /gene="pta"
                     /locus_tag="PLX80_13850"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HQY21920.1"
                     /translation="MDLYKELLRKSKNNRKTIIFPESNDERVISAVKKILKLKCAKVI
                     LIETGENILKKIPDNKDLLIIDKEFSSQFVKDYHKIKLIRKPEQNIEQSESELQDPLT
                     FSCMLLKNGYADGIIAGSVYSTSDVLRNSISIIGVSKNNKTVSSFFLFTFPAKHKFNE
                     KIFTYADCGVVPNPNIEQLSDIAISTADNFKKLTGIKPKVAFLSFSTKGSAKSDSTEK
                     VIKAVELTSKRSPTLIVDGELQFDSAFVPEVAKRKNPKGKIKGDANVFIFPDLNSGNI
                     AYKITERIGGAIATGPIIQGLSKPVMDLSRGCSAEDIFNMSLIISNIN"
     gene            complement(2453..3556)
                     /gene="mnmA"
                     /locus_tag="PLX80_13855"
     CDS             complement(2453..3556)
                     /gene="mnmA"
                     /locus_tag="PLX80_13855"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQY21921.1"
                     /translation="MNNSNNNKTVVIAMSGGVDSSVAAALLKKDGYNLIGITMKTWGY
                     DDFPEKDSGCCSLETIYSARNVAQNLGFSHYTLDFTEKFNEIVIENFISEYLKGNTPN
                     PCVLCNKSIKWGALLEKAESLGADYLATGHYAKIGVNENNKRYFVSTAEDKKKDQSYA
                     LWRVSQYALSKTLFPLGEYNKSEIRSLAHEMNLKPADTPDSQEICFVPNDNYKDLIQI
                     RLPGLNEKLDGGDIIYKDKKVGTHKGYPYFTIGQRRGVNVALGKKVYVSKIDAENNQV
                     FIDDEESLYNSEFTVKEINLMMTDKIEKPMKSRVKIRYNDVGKYAVLDQISDTEIKVI
                     FEEPQKAITPGQSAVFYDGENVFGGGIINKILQ"
BASE COUNT         1159 a          649 c          567 g         1348 t
ORIGIN      
        1 ttgaaattat ttttttggtt tggggaaagt tcgggatttt gtgtcgtgta atttaattta
       61 caaggcataa tactttattt acaaataagt aattttattc agtttcatat atatatgaat
      121 agtcttaatt tacaattctt ttttacatac taataaataa tgatagaaat aaaaaatctg
      181 aaaaaaagct tcggcaataa agaagtttta cgcggagtaa accttaccat tgaagacggt
      241 attacacttg ttattatagg aagaagcgga tgcggtaaat ctgttttact taaacacatc
      301 atcggacttc taaaacctga tgaaggtgaa gtattgatag aaggaaatga tattgcaaaa
      361 atggatcaga aggaattata cgaaattcga aaaagattcg gatttctttt tcagggagct
      421 gctctttttg actctatgga tgtggaggaa aatataggtc tcgcattaaa ggaaaataca
      481 agcatgacaa aatctgaaat tgctgatgta gttgttgaaa aacttgaact tgtcggtctt
      541 cccgggacac agaatatgaa accggcagat ctttccggag gaatgaagaa aagagtttct
      601 cttgcaagat ctcttgctac taatcctgag tatattcttt atgatgaacc tacaaccgga
      661 ctggatcccg tcatgagtga tcagatagat gacctcatac aggacctttc aaaaaaactt
      721 aaagttactt ctatagttgt cacgcacgat atttttagtg tttatgatat tgcagatgta
      781 gtagctatga tgcatgaagg aaggatttat tttatcggaa caccaaagga gctgacaact
      841 actgatgata aacttattcg tgattttctt aacaggactg ataaaaggat ctagaacata
      901 taattgaaat ttcttagaga atacttattt agatttcttc ataaagagta tttgtgaaac
      961 tgaatttcgc ctgataactc caattccaaa cagagtttgg aaacgagcgg taatgttttg
     1021 tattcagctt aataaatttt caataaattc ttctttacta aggtaactca acttctcctt
     1081 cttcagcatt ccagcctttc cagcctccgt ccattgaatg tacatttgta taacccattt
     1141 tttgcagcga ctctgccgct atagcagaac gatatccacc accgcagtaa agtacaatct
     1201 tcttatcatg atcatttaca aaaagatgaa tgtctctttc gataattcct ttccctaaat
     1261 gtactgctcc ttttatcctt cctttttcaa attcattatc ttcccgaatg tcgatcagta
     1321 tatgatcttc attatttttt ttcatttcca ttacatcttt tactgtacat tcggtaatat
     1381 tctttttgat tatttcacaa agctcatgaa aggtcttctt cattttatta aaaattaatt
     1441 tatatttgaa attattaaac tcatattgaa aatatcctca gcactgcatc cacgggacag
     1501 gtccatcaca ggttttgaaa gtccctgaat aattggaccg gttgcaattg ctcctcctat
     1561 tctttctgtt attttataag caatgtttcc tgaattcaga tcagggaata taaatacatt
     1621 agcatcacct ttgatcttac ctttcggatt ttttcttttt gctacctccg gaacaaatgc
     1681 cgagtcgaat tgtaattcac cgtcaactat taaagtcggt gaccttttac ttgtaagctc
     1741 aactgcttta attacttttt ctgtcgaatc acttttagcg ctgcctttag ttgaaaatga
     1801 aagaaatgcg actttgggtt ttataccggt aagcttttta aaattatcag cagttgatat
     1861 tgcaatatca gacaattgct cgatgttagg atttggcact actccgcaat cagcatatgt
     1921 aaaaattttt tcattaaatt tgtgcttagc tggaaaagta aataaaaaaa atgaagatac
     1981 tgttttatta ttttttgata ctccaattat agatattgaa tttcttaaaa catccgaagt
     2041 actgtaaaca cttcctgcga ttattccgtc agcatatcca tttttcaaaa gcatacagct
     2101 aaatgtcagc gggtcttgaa gttcagattc agactgttca atattttgtt caggctttct
     2161 tatcagtttt attttatgat aatcttttac aaactgagaa ctaaattctt tgtcaattat
     2221 aagcagatcc ttattgtcag gaattttctt taaaatattt tcacccgttt ctataagaat
     2281 tactttagca cattttagct ttaaaatttt tttaacagca gatatgactc tttcatcatt
     2341 agattcaggg aagataatgg tcttacggtt atttttgctc tttcttaaaa gctctttata
     2401 tagatccatt aaaaatgcat tatttaatat ttaatattta atatttaata ttttactgta
     2461 atattttatt aataataccg ccaccgaaaa cattttcacc atcataaaaa actgctgact
     2521 gaccgggcgt aatagctttc tgaggttcct caaaaatgac tttgatctca gtatctgaaa
     2581 tttgatcaag tacagcatat ttaccaacat cattatatct gatctttact ctggatttca
     2641 tcggcttttc aattttatcg gtcatcatca gattaatttc ttttacagta aattcagaat
     2701 tataaagact ttcctcatca tctataaaca cctgattatt ctcagcatcg atctttgata
     2761 catagacctt ttttcccaat gcaacattaa ctccccttct ttgcccaatt gtaaaatatg
     2821 ggtaaccttt atgcgtcccg acttttttat ctttataaat aatatccccg ccatctagtt
     2881 tttcattcag tccgggcagt ctgatctgaa tcagatcttt gtaattgtca tttgggacaa
     2941 aacatatctc ctgtgaatcg ggagtatcgg caggtttgag attcatttca tgtgcaagcg
     3001 aacggatctc ggatttatta tattccccaa ggggaaacag agttttactc aaagcatatt
     3061 gagatacacg ccataatgca taagactgat cctttttttt atcctcagcg gtcgagacaa
     3121 aatatctttt attattttca tttacaccga tctttgcata atgtccggtc gccagataat
     3181 ccgctccaag agattcagct ttttcgagta atgcacccca cttgatagac ttattacaaa
     3241 gtacacacgg gtttggagta ttacctttca gatattctga aatgaaattt tcaattacaa
     3301 tctcattgaa tttttcagta aaatccaaag tatagtgact aaatccgaga ttttgagcta
     3361 catttcttgc actgtatatt gtttccagtg agcagcatcc ggaatccttt tcgggaaagt
     3421 catcatagcc ccaggttttc attgtaattc cgatcagatt atatccatct tttttaagta
     3481 atgctgcagc tacagaagag tcaactccgc cgctcattgc aattactact gtcttattat
     3541 tattagagtt attcattttt atttaaagta aaatatgaat tggcatttat ttaaaaaagt
     3601 taaaaattgt attgaacttt taacattatt gaggtagttt gattctgaca taaattaaac
     3661 aaggagaaaa acatgtcaaa aagatcatta ttaatattgt cttttttatt tgtttttact
     3721 ttt
//
LOCUS       DAORVQ010000267         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20323_length_3692_cov_6.100082, whole genome shotgun sequence.
ACCESSION   DAORVQ010000267 DAORVQ010000000
VERSION     DAORVQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20323_length_3692_cov_6.100082"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLX80_13860"
     CDS             complement(<1..196)
                     /locus_tag="PLX80_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21922.1"
                     /translation="MKAFKKIFDPNKLPTLLFYSSLVAFIIAFNFAHNPPGGWTQQFM
                     PDLNNRPIVDITFTDSLTGYA"
     gene            complement(712..2043)
                     /locus_tag="PLX80_13865"
     CDS             complement(712..2043)
                     /locus_tag="PLX80_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21923.1"
                     /translation="MKKLFVIFFAFIFCNGFTQWTVVSTVPTSALINSISVVNENLIW
                     VACDGGRVYKTTNGGVNWNLRNTGLPSGNVYGISALDTSNCWVGNISGSIYRTSDGGA
                     SWTLQLAVANSFSDGIHMFNANYGIYYADPTAASGQTFQYRYTTNGGTNWILAANVPN
                     VSNEYGILNAWDWLDSNTIWLGVANVVSGAASSKVYKSNGGFITGNFTFGQFGGAPSS
                     DGLYSQGVGFTDANNGLIGTNNGQMSRTINGGSTWQIVTNPVGIVSYAVNDMHSFKDG
                     SNIIRVAMSTTVSDSAWVYRTNDLGVTWIRETIPPQALANEIQHMEFVNQTTGYAGCA
                     GGLFLKYTGPSGITNLNGIQPSEYKLDQNYPNPFNPSTKINFSIPASSNVKLVIYNAL
                     GKEVSTIVNEFKNAGNYSVEYSAAGNLNSGVYFYTLSAGDFVSTKKFVLVK"
     gene            complement(2166..3527)
                     /locus_tag="PLX80_13870"
     CDS             complement(2166..3527)
                     /locus_tag="PLX80_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21924.1"
                     /translation="MKNFKQFFTSLFILALALSINSDVYSQWTLAGLVTNAGTSPSIS
                     VVDQNIVWVAGGPTGTPVVSRSTNAGANWTNVPTSQLPLEVYCVWGIDANTCYVGNGG
                     AAGGAGGNAQYYKTTDAGVTWTVVGSTGGSAGFFNGVVFSKTMPSFGVAQSDPPTGAG
                     QSYYLGITTDGGLTWTPTTATGFAGTASAQNSIVVIDNMFYGFGSNTTPPAFIYTSNG
                     GTSWQKSNTGAPGNFTSGVAFSDDKMHGLVSSSTSLPTIARTTNGGVSFTNVTGVAGL
                     TGYSTIKWIEGTNTVYTSSAIGASGVVQKSTNGGLNWTVMSTSSLTGITHMEFRRVGN
                     TVYGFAVTANGAVLKLVETITGVNNISSSVPDGYSLDQNFPNPFNPSTTINFSIPVTG
                     KVNLTVYNSLGKEVATLVNENLAAGSYSSEFTAASDLTSGIYFYTITSGNFSDTKKLM
                     LVK"
BASE COUNT         1151 a          691 c          639 g         1211 t
ORIGIN      
        1 ttgcataccc tgtcagacta tcagtaaaag taatatccac tataggtctg ttattcagat
       61 ccggcataaa ctgctgcgtc cacccgcccg gcggattatg cgcgaaatta aatgcgatga
      121 taaaagcaac taagctcgaa tagaaaagaa gtgtcgggag tttattggga tcgaatattt
      181 ttttaaaagc tttcatattt tttttcttaa aacgtacaaa gtaagaaatc aaaacacaat
      241 atttaaatac atataaatac tgattcgttt ttctcaatta aaattcacaa tgaatttttg
      301 ggggtgaagt tttaaagggg tttctaactt taattttgtt tttacactgc tatttataaa
      361 ttatactatt taaaatcaag ataatatttt acacgggtcc gttggtctga accttgatta
      421 taggattatc atgattactt gattttagct tatgttggtg aaatccgctg tttcaatggc
      481 aggatagagc cggcttctac ctttggagtg gatgggaatg ttctcacagc tgcaggagac
      541 acgtctccgt tttttgctgg tgatcaaaaa gtttgcttaa gatttgcagt aatattcaac
      601 tgatcctcag gcaaactttt tgaaaacacc agcaaccgct gaaaataaaa aaaccctcca
      661 aatatatctt tagatataat aagaagggtt atataaaaaa tgttttctgt attattttac
      721 taatacaaat ttcttagttg agacaaaatc acctgccgaa agagtataga aataaacacc
      781 ggaatttaaa ttacctgctg cagaatattc tactgaataa ttgcctgcat ttttaaattc
      841 attaacgatc gtactaacct ctttaccaag tgcattataa attaccagct tcacatttga
      901 agaagccggt attgaaaaat tgatctttgt agatggatta aacggattcg gataattctg
      961 atctagttta tattcagaag gctgaattcc gttcaaattt gtaataccgg aaggtccggt
     1021 atatttcaaa aacaaacctc ctgcacatcc tgcatatcct gtcgtttgat ttacaaattc
     1081 catatgctga atttcatttg caagagcttg aggtggtata gtttctctaa tccatgtaac
     1141 tccaagatca tttgttctat aaacccatgc agaatcagat actgttgtac tcattgcaac
     1201 tctgataata tttgagccat ccttaaaaga atgcatatcg tttacagcat atgagactat
     1261 accaacagga tttgtaacga tctgccatgt gctgccgccg ttgattgttc tgctcatctg
     1321 accattgtta gttccgatca aaccattatt tgcgtcagta aaaccaactc cctgtgaata
     1381 taaaccatct gaggacggag caccaccaaa ttgtccaaaa gtaaaattgc cggtaataaa
     1441 gccaccatta gatttatata cttttgatga tgctgcacct gatacaacat ttgctactcc
     1501 aagccagata gtattactat ccagccagtc ccatgcattt aaaattccgt attcatttga
     1561 aacatttgga acatttgcag cgaggatcca gtttgtaccg ccgtttgtag tatatctgta
     1621 ctggaaagtc tgacctgaag ctgctgtagg atctgcataa tatattccat aattagcatt
     1681 gaacatatga ataccgtcag agaagctgtt tgcgactgca agctgtaaag tccatgaagc
     1741 accgccgtcg ctggttctgt aaatagaacc gctgatattt cccacccagc agtttgatgt
     1801 atctaaagct gagataccat aaacatttcc agatggaagt cctgtatttc tgagattcca
     1861 gttgactccg ccgttagtgg ttttataaac tctgccaccg tcgcatgcaa cccagatcag
     1921 attttcatta actacagaaa tggaattaat aagtgcagat gtaggcacag tgctgactac
     1981 tgtccactgt gtaaatccgt tacaaaaaat aaatgcgaaa aaaattacaa ataatttttt
     2041 caaagtatat cctttctttt ttaaatttaa tattatttgt aattagctgt tacgttggat
     2101 gtaaaaacac ttaaagaccc gtctgttctg gaacgggtct ttaagattta ttttaaaact
     2161 atgaattatt ttaccaacat caatttcttt gtatcgctga agtttccgga tgtgattgtg
     2221 tagaaataaa taccggatgt aagatctgaa gctgcagtga attctgatga ataacttcct
     2281 gctgctaaat tttcatttac aagtgttgct acttcttttc ctaatgaatt ataaactgtt
     2341 aaatttactt ttcctgtaac aggaattgag aaatttatag tagtggatgg attaaatgga
     2401 ttagggaaat tctgatcaag tgaatatcca tcaggtactg aactgctaat attatttaca
     2461 cctgtaatag tttcaactaa ttttaaaact gcaccatttg cagtgactgc aaatccgtaa
     2521 acagtatttc caactcttct gaattccatg tgtgtaatac ctgtcagact tgaagtactc
     2581 atcacagtcc agttaagtcc gccgtttgta cttttttgaa caacacctga agcacctata
     2641 gcactggatg tatatactgt attcgtacct tcaatccact taattgtact gtagccggta
     2701 agtcctgcaa ctccggtgac attagtaaaa gaaacaccgc cgttagttgt tcttgcaata
     2761 gttggaagcg atgtacttga agaaactaat ccatgcattt tatcatcact gaatgctaca
     2821 cctgaagtga agttaccagg tgctccggta ttactttttt gccaggatgt tccaccgttt
     2881 gaagtataaa taaatgccgg aggagtagta ttgcttccaa atccgtaaaa catattatct
     2941 attactacaa tagaattctg agctgaagct gtacctgcaa aacctgtcgc agttgtaggt
     3001 gtccatgtca gaccgccgtc agttgttata ccaagataat atgactgacc tgctcctgtt
     3061 ggtggatcac tttgagcgac accaaaagat ggcattgttt ttgaaaatac tacaccgttg
     3121 aaaaatcctg ctgatccgcc tgttgaaccg actactgtcc atgttacacc tgcatcagtt
     3181 gttttataat attgagcatt tccacctgct ccgcctgctg ctccgccgtt tccgacataa
     3241 caggtatttg catcaatacc ccagacacaa taaacttcta aaggaagctg acttgtcggg
     3301 acgtttgtcc agtttgcacc tgcattggtt gatcttgata ctaccggtgt accagttggt
     3361 ccgcctgcta cccatactat attctgatcc acaacagaaa tactcggtga agtacctgca
     3421 tttgttacaa gtcctgcaag tgtccattga gaataaacgt cggaattaat acttaaggct
     3481 aaagctaaga taaatagaga tgtaaaaaat tgtttaaaat ttttcatttt ttgctcctga
     3541 ttatagttta tttaataaat tattttttaa aaatattttg acttaattta caaattattc
     3601 aataataaaa cagtgttaaa tatttgtgaa ttttttgaaa atgttgttaa aatatgctgt
     3661 agtttttatt aaaaaaattt gcgaattaaa tt
//
LOCUS       DAORVQ010000268         3686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20382_length_3686_cov_5.226384, whole genome shotgun sequence.
ACCESSION   DAORVQ010000268 DAORVQ010000000
VERSION     DAORVQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20382_length_3686_cov_5.226384"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..2138)
                     /locus_tag="PLX80_13875"
     CDS             complement(18..2138)
                     /locus_tag="PLX80_13875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21925.1"
                     /translation="MTGRAVSVILPHDYQWYVDYSGNKIAKINRDTYDALNGTGINLD
                     WQLFDKWTYDSLGNFTFAADGRVDMLLKVHRHDPNKLLHPTAGGYIPLGACEGADWNN
                     EYTVYQSGSTAVKINGNTGGNGSGGVVCPKGSITDREFYLAVTTHEMGHYLWGHGHHQ
                     YCKMAGGMGGEFGLSPWEVVKLEYIEQQIVNYTNPNYYLSDYMSRGGTSGTEGEILQV
                     PINESGSQFFLLANRGNLSEWDKKMGGDTVAGYFLKDINEDYGKGVYIYHVIGGYSEP
                     SGNEQEIDLECADGLWDWTQQGTSSPLWNQTIIHPVFVKTDVKYDQDNPAIYDHIGKD
                     GMSAWVTGIVNGNNYTQGPWYSEGKSHTYSPLNHGTDRVYTNDKDFWYSYQVAGDRYD
                     PWNIGYNELFSPYSSPNSKDSANQYTGIYIWMYSSSGSGPTSSAAFKIFKAGEGGYTD
                     SSILQITPPSRPMGLNVMPCDSQPTINYYKRIKLKWNHNMEPDMERILIGEEGLFKRY
                     KIYRSTASDMGSVPPDALQNSENYYTCIDTIDILNSSEPEYIDAQLVSICNTSACEAP
                     NFCIEYPVRYRVQAVDKYEDVSVLSDFVSTRAGSLEIGPRGNFNLSETENELPSEYSL
                     RQNYPNPFNPSTNIQYDLPADNFVTIKIYNTLGKEVLNLVNEFKNAGRYIVSFRGAGL
                     SSGVYYYRIKAGSFEQIRKMILLK"
     gene            complement(2135..2428)
                     /locus_tag="PLX80_13880"
     CDS             complement(2135..2428)
                     /locus_tag="PLX80_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21926.1"
                     /translation="MKKLIFIFLSVLFFLTYHNSFPQPYDYSCGTEIISGQSPTSSIF
                     IGGKYKPHRTDIGGASTGTDHFPVLVVFVQFQNEPGDTSNMDPNYWSREENFM"
     gene            complement(2608..>3686)
                     /locus_tag="PLX80_13885"
     CDS             complement(2608..>3686)
                     /locus_tag="PLX80_13885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21927.1"
                     /translation="IYKTTNGGINWINQPNSSTSFIQQIHPVNDSVVYAAGWWNFLKT
                     TDGGNNWISIFSGGPGMGLPTLDGIFFIDENTGWMCGQVTIMKTTDGGNSFIDSGRIE
                     GLPMDIYFKNENEGIMSSQFGFVFRTINGGKDWITERIIKSGSQYNFLKVSFVNDSVG
                     YVGGEIVFKTTNFGVTWDSVGAVPFGVEEAYCIEFADENTGYCGGTAGTLFKTTDGGG
                     TWVQMQGSFFNNGYFRSIYAYSDLIVWAVGNGKFLFTETGGLVNIEHLSAKIPGDFVL
                     YQNYPNPFNPKTKIKFDVKNISHVNLEVFDMLGRKVSTLINERLQTGTYEAEFEIPDQ
                     NSGVYFYKLSAENFSKTMKMILLK"
BASE COUNT         1105 a          755 c          565 g         1261 t
ORIGIN      
        1 attgaaagtt ttagaattta tttcaaaaga atcatcttcc gtatctgctc aaagctgcct
       61 gccttaatcc tgtaataata tactccgctt gatagacctg ctcctctgaa agatacaata
      121 tatctgcccg catttttaaa ttcattaaca agattcaata cttctttacc aagtgtatta
      181 taaattttta ttgtaacaaa attatcagcc ggaagatcgt attgaatgtt tgtacttgga
      241 ttaaatggat taggatagtt ttgtctcagc gaatattctg aaggtaattc gttttcagtt
      301 tccgataaat taaaattacc tctcggtcca atctctagac ttccggcccg tgtactgaca
      361 aaatctgata aaacagaaac atcctcatat ttatccactg cctgaaccct gtatctgaca
      421 ggatattcaa tacaaaaatt aggagcttca catgcagagg tattgcaaat tgaaacaagt
      481 tgtgcatcaa tatattccgg ttcagagctg ttaagaatat ctattgtatc aatacaagta
      541 taatagttct ctgagttttg taaagcgtca ggaggaacag atcccatatc tgatgcagta
      601 cttctgtaaa ttttatatct tttgaataat ccttcctcac cgattaaaat tctttccata
      661 tcaggttcca tattatgatt ccatttaagt tttattcttt tatagtaatt tattgtcggc
      721 tgagaatcgc agggcattac atttagtccc attggacggg atggaggtgt gatctgaagg
      781 attgaagaat cagtataacc gccttcgccg gctttgaata ttttaaatgc tgctgaactt
      841 gtaggacctg atccggaact agaatacatc caaatgtaaa ttcccgtata ctgatttgca
      901 ctgtcttttg aatttggact cgaataaggg gaaaataatt cattatatcc gatattccat
      961 ggatcataac gatctcctgc aacttgataa gaataccaaa aatctttatc atttgtatat
     1021 actctgtctg taccgtgatt cagcggacta taagtatggc tctttccttc agaataccaa
     1081 ggtccttgtg tataattatt tccatttact attcctgtta cccaggcact cattccatct
     1141 tttcctatat ggtcataaat cgctggatta tcctgatcgt attttacatc cgtttttaca
     1201 aaaaccggat gaataatagt ttgattccat aggggacttg aagttccttg ctgtgtccaa
     1261 tcccataaac cgtcagcaca ttctaaatca atttcttgct catttccgga tggttcggaa
     1321 tatcctccga taacatgata tatataaact ccttttccat aatcttcatt tatatctttt
     1381 aaaaaataac cggcaaccgt atcacctccc atttttttat cccattctga taaatttcct
     1441 ctgtttgcca aaagaaaaaa ttgacttcct gattcattta taggaacttg taaaatttct
     1501 ccctctgttc ctgaagtacc gcctctggac atataatcag ataaataata attcggattg
     1561 gtgtaattaa cgatttgttg ttcaatatat tcaagtttta ccacttccca gggacttagt
     1621 ccgaattcgc ctcccattcc ccctgccatc ttacaatatt gatgatgacc atgtccccat
     1681 aaatagtgtc ccatttcatg tgtagttaca gcaagataaa attctctgtc tgtaatactt
     1741 cccttaggac atacaactcc gccggatcca ttacctcctg tatttccgtt aatttttaca
     1801 gctgtacttc cgctttgata aactgtatat tcattgttcc agtctgcgcc ttcgcacgct
     1861 cctaacggaa tataacctcc agccgtagga tgcaacaatt tgttaggatc atgacgatga
     1921 acttttaaaa gcatatcgac ccgtccatct gcagcgaatg taaaatttcc taaactgtca
     1981 tatgtccatt tatcaaataa ttgccaatct aaatttattc ccgtaccatt taaagcatca
     2041 taagtatcac gatttatttt agcgatttta tttccggaat aatctacata ccattgataa
     2101 tcatgcggaa gtattactga taccgctctt ccggtcacat gaaattttcc tctcgactcc
     2161 agtaatttgg atccatatta cttgtatctc cgggttcatt ctgaaactga acaaatacaa
     2221 cgagaactgg aaaatgatca gtaccggtcg aagctccgcc aatatccgtt ctgtgaggtt
     2281 tatatttgcc gcctataaat atagaagaag tcggtgactg accgctaatt atttcagtac
     2341 cgcagctata atcataaggc tgcgggaagg aattatgata agttaaaaaa aatagaacgc
     2401 ttaaaaatat aaatattaat tttttcattt tcgcttaatt taatggttta aaaaagttat
     2461 tttagaaaat taaaaatggg cgcagcaatt ctgcaaatta agaattactt ttaaatttaa
     2521 acgttgtccg cgtttgttat aaagtgagct gtcgccctaa ttttttatta cttaccatgg
     2581 tggagtattc ataattgacc ctccggttta ttttagcaga atcattttca tagttttact
     2641 gaaattctct gcgcttaatt tataaaaata aactcctgaa ttttgatccg gtatttcaaa
     2701 ttctgcctcg taagttcctg tttgcagtct ttcatttatc aatgtagaaa cctttcttcc
     2761 gagcatatca aaaacttcaa gatttacatg acttatattt tttacatcaa attttatttt
     2821 agtttttgga ttaaaaggat taggatagtt ttggtaaagt acaaaatcac ctgggatttt
     2881 tgcacttaaa tgttcaatat ttactaatcc tccggtttcc gtaaagagaa atttaccgtt
     2941 tcctacagcc cagacaataa gatcgctgta ggcgtatatg ctcctgaaat atccattatt
     3001 gaagaaagaa ccttgcatct gcacccatgt cccgcctcca tctgtagttt taaagagtgt
     3061 cccggcagtc cccccgcagt accctgtatt ttcgtcagca aattctatac agtatgcttc
     3121 ttcaacacca aaaggaactg cgccaacact atcccatgtc acgccaaaat tagttgtttt
     3181 aaaaactatt tcaccaccga cgtaacctac gctgtcattt acaaaagaaa ctttaaggaa
     3241 attatattga ctgccagatt ttataattct ttccgttatc caatctttac cgccattaat
     3301 tgttctaaaa acgaagccaa attgtgaact cattatacct tcattttcat ttttaaaata
     3361 aatatccatt ggtaaacctt caattcttcc tgagtcaata aatgaatttc cgccgtcagt
     3421 ggttttcata atagtaacct gtccgcacat ccagcctgta ttttcatcta tgaaaaatat
     3481 gccatcgaga gttggaagtc ccattcccgg tcctccgctg aaaatggaaa tccagttatt
     3541 gcccccgtct gttgtcttta aaaaattcca ccagcctgct gcatatacta cactgtcatt
     3601 gacagggtgg atctgttgaa tgaaagaagt agaagagttt ggttgattta tccaatttat
     3661 tcctccgtta gtagttttat atataa
//
LOCUS       DAORVQ010000269         3682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20410_length_3682_cov_3.876482, whole genome shotgun sequence.
ACCESSION   DAORVQ010000269 DAORVQ010000000
VERSION     DAORVQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20410_length_3682_cov_3.876482"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            689..1390
                     /locus_tag="PLX80_13890"
     CDS             689..1390
                     /locus_tag="PLX80_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21928.1"
                     /translation="MNSTKLLLILFTLYLTVSISAAGCGKGEVSQKTGTVKKDSSDLN
                     SGSQNANSSITDQTDFPPVTLDFKTATGKFYLVIIKKNSESLIDIMISGLGFENSNDT
                     MIYKDSDPFDKALMGDLDGNGFDDFLVVTRSAGSGSYAGLYGVASNDDKSFTQINFPP
                     VTEADMQPGKPFEGYMGHDFFNVEDNVLIREFPVYRSGDPNSSPTGGKRRISYKLSQG
                     ETMYQLVVDKVEKLE"
     gene            complement(1451..2260)
                     /locus_tag="PLX80_13895"
     CDS             complement(1451..2260)
                     /locus_tag="PLX80_13895"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQY21929.1"
                     /translation="MIEPREISNTASKKKLREVQIEKDYIIGWFLRGISKNKILNEML
                     IFKGGTALRKIYFDDFRLSEDLDFTYAGDNMDTKEIKNEFTIVTDWIEIESKIKAEVE
                     KEITNSFGNYSFDLKYTGPLGGSDKSIKVDISENEILVNKTIHKEIRDEYSDSEKNCK
                     ILCYSIEEIISEKLRSLMQRTAPRDLYDIWFLIVKEGNNIEDYVSDFIKKANFKDINP
                     KNFKSKIEEKKDKFKSSWENQLKNQMNDVPDFENVWRKLNFHFKKFEKGLK"
     gene            complement(2264..3079)
                     /locus_tag="PLX80_13900"
     CDS             complement(2264..3079)
                     /locus_tag="PLX80_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY21930.1"
                     /translation="MDKNISFRSAEVLRVLNESNQTFFIVGDVEKILKGSSSQNVRKL
                     LSDMTKRKLIMRLKDGLYTIIPYEKTHDYFPNWHLVAEKLAYPNKYYIGFYSALDLHG
                     LITQPSLTEYIVSNKQFIPKKQIIKNIKFEFINYNEQHFFGFEKKWINNYDKVYCSDL
                     EKTIIDCLYKPQFASGITEIVKAINRCKKEINSDKMFEYLYNFNANVVFKRLGFILEN
                     FPEFNSLTNSLYKKVSSSKAYLDPTYPKKGIYNSRWKIIDNVGIDEALNSITT"
     gene            complement(3214..3381)
                     /locus_tag="PLX80_13905"
     CDS             complement(3214..3381)
                     /locus_tag="PLX80_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21931.1"
                     /translation="MDTLSKKEKKIAKEIIDKGLLIEFGNALNDEDAVLKKWKDKEIM
                     IRKNLWRNTRE"
BASE COUNT         1070 a          757 c          541 g         1314 t
ORIGIN      
        1 aaaataccat aaagtaaaaa atcctcctgc ttttttccaa caatcaattt tataaaaata
       61 ccctcctttt ctttgttact ttcgagcctg ccctcaagtg ctcctatcgg ggggacgtaa
      121 aaaagaaagt aagcagaaac ctgttaatcc gaaattttga aataaccgtt ttagacagag
      181 ctgacttcca aaccattttg caagctcctt cccaatctcc cggactgtcc gaaaacaaaa
      241 attacaattt gcttacaagg ttgcctccgg gactcgaaga ttcaaagaaa ataatttttg
      301 aaatttttaa actttaagtc ttagagtctt agagtctttg agtctttgag tcttagagtc
      361 tttgagtctt tgagtcttag agtcttagag tcttagagtt ttagagtttt agagtcttag
      421 agtcttagag tcttagagtc ttagagtctt agagtcttag agtcttagag gtaaactgtc
      481 cggattggga aagcaaacac ctctccgtgt ctccgtgcct ccgtgtctcc gtgtctccgt
      541 gtctccgtgt ctccgtgtct ccgtgcctcc gtgcctccgt ggtaaatacc aaaccacacc
      601 cgaaattaat ttcccaccga atgcagcttc attttcaaaa tattattcat aattttaccc
      661 ccaaaacatt ttatccaaac acactgacat gaattcaact aaacttttgt taatactctt
      721 cacactctac ttaacagtct caatctccgc cgcaggctgc ggaaaaggag aagtttccca
      781 aaagaccggc actgtgaaaa aggattcctc cgatttaaat tccggttcac aaaacgcaaa
      841 cagcagcatc acagatcaaa ctgacttccc gcccgtgacc cttgatttca aaaccgctac
      901 gggaaagttt tatcttgtga tcataaagaa aaacagcgaa agtctgattg acatcatgat
      961 ctcgggactg ggatttgaga attcgaatga caccatgatc tataaggaca gcgatccttt
     1021 tgacaaagct ctcatgggag atctggacgg gaacgggttc gatgatttcc tcgtcgtcac
     1081 gcgcagcgcg ggatcaggtt cttatgccgg actctatggt gttgcatcca atgatgataa
     1141 aagttttacg cagataaatt ttcccccggt caccgaagcc gatatgcagc ccggtaaacc
     1201 gttcgaagga tacatgggac atgacttttt caatgtggaa gataatgtgc tcatccggga
     1261 attcccggtc tacagatccg gcgatccgaa cagcagtccc accggcggca aaagaaggat
     1321 cagttacaaa ctctctcagg gcgaaacaat gtatcagctg gtggtggata aggtggagaa
     1381 attggaatga ataaagcttg agttatttaa tatagatcat ctgtttatga atttataatt
     1441 aaaaatgttt ttactttaat cccttttcaa atttcttaaa atgaaaattt aatttcctcc
     1501 atacattttc aaaatccgga acatcattca tttgattctt caactgattt tcccatgaac
     1561 ttttgaattt atcttttttc tcttcaattt tggatttaaa atttttcgga tttatatctt
     1621 taaaatttgc ctttttaatg aaatccgaca cgtaatcctc tatattattt ccttccttaa
     1681 caattaaaaa ccaaatgtca tacaaatctc ttggagccgt tctctgcatt aaagacctca
     1741 acttctcaga aataatttcc tctatagaat aacataaaat tttacagttc ttttctgaat
     1801 ccgaatattc atccctaatt tctttatgaa tagttttatt tactaaaatt tcattttcac
     1861 ttatatcaac ttttatactt ttatcactac ctcccaaagg tccggtatat tttagatcga
     1921 aactatagtt tccaaaacta tttgttattt ctttttcaac ttctgcttta atttttgatt
     1981 ctatttcaat ccagtctgtt acaattgtaa actcgttttt aatttctttc gtgtccatat
     2041 tatcccctgc ataggtaaaa tccagatctt ccgataatct gaaatcatca aaatatattt
     2101 tccggagcgc agtaccgcct ttaaagatca gcatttcatt aagtattttg ttcttggaaa
     2161 tacctctcaa aaaccagcca attatataat ctttttcaat ttgaacttcc cgcagctttt
     2221 tctttgatgc tgtattagaa atctctcttg gttcaatcat acattaggtt gtaatagaat
     2281 ttagagcttc atcaattcct acattatcta ttattttcca tctgctgtta tatattcctt
     2341 ttttggggta agtaggatcc agataagctt ttgatgagct gacttttttg taaagagaat
     2401 ttgtaagtga gttaaattcc ggaaagtttt ccagaataaa tcccaatctt ttgaaaacca
     2461 cattagcatt aaaattataa agatattcaa acattttatc tgaattaatt tcttttttac
     2521 atctgttaat tgcttttaca atctcagtta taccggatgc aaattggggt ttataaagac
     2581 agtctataat ggttttttca agatcactgc agtacacttt atcataatta ttgatccact
     2641 ttttttcaaa tccgaaaaaa tgctgttcgt tgtaatttat aaattcaaat ttaatatttt
     2701 ttatgatctg ttttttggga atgaattgtt tattggaaac aatatattct gttaatgacg
     2761 gctgcgtaat taatccatgc agatctaaag cactgtaaaa tccaatataa tatttattgg
     2821 gatatgccaa tttttcagct actaaatgcc agttaggaaa atagtcgtgg gttttttcat
     2881 aaggaataat ggtatatagg ccatctttta atctcattat cagtttccgc ttggtcatat
     2941 cactaagcag ttttcttaca ttttgactgc tgcttccctt caatattttt tcaacatccc
     3001 ccacaataaa gaatgtttga ttgctctcat ttaacactct taaaacctct gcagacctga
     3061 aagaaatatt cttatccatt tattaaactt tttatattaa tattgtgagt tgataactca
     3121 catatccaat ataaaacatg tagattttct ttccaatgta ctaaaacaat ttattccaat
     3181 ctaaattctt tatttaatac ttccctattt ttctcattcc cgggtgtttc tccacaaatt
     3241 cttccggatc ataatttcct tatccttcca ttttttcaaa actgcatctt catcgttcaa
     3301 tgcattccca aattctatca gcaatccctt atcaattatc tccttcgcaa tttttttctc
     3361 tttttttgaa agtgtgtcca tttaatattt aaattgtaag attctatcat ctcttatttt
     3421 ctgtcatcct cctttttttg tcatccccct ccttttctgt cattctctcg tgctgctaat
     3481 tttgtcatcc ccgcgtgctc ctaattttgt cattcccgca tgctcccggc gggaatctaa
     3541 aatcgaaacc caagccaaac aatctcagca ttcctaattc gtaattctta attcgtaatt
     3601 ctaaattgat tttgtcatcc tgagcgaagc gaaggatcca cgaatgttgc gaccaccttt
     3661 gccttttgta attttgtgcg ca
//
LOCUS       DAORVQ010000270         3656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20669_length_3656_cov_5.196890, whole genome shotgun sequence.
ACCESSION   DAORVQ010000270 DAORVQ010000000
VERSION     DAORVQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20669_length_3656_cov_5.196890"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..950)
                     /locus_tag="PLX80_13910"
     CDS             complement(351..950)
                     /locus_tag="PLX80_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQY21932.1"
                     /translation="MKNLGSIFFKYRSYIPLPFVFTMILFMEPTLYTIIIGFIIALTG
                     EIIRIWSVSFAGSETRTTSGVGGTYLVTQGPYSVIRNPLYLGNIMIYIGIGIMSNALF
                     PYLQIFALFFFAFQYHCIVLAEEEFLQSKFTNVYSDYVKYVGRFFPKFKKLPSHIISD
                     LELNVKSGFKSERRSLQAFLITSAIIIIYYLLKVCFKVI"
     gene            complement(1003..1740)
                     /locus_tag="PLX80_13915"
     CDS             complement(1003..1740)
                     /locus_tag="PLX80_13915"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21933.1"
                     /translation="MKSYRLKIFCEFRIILSDSDSVINSLKNFIPENKLKRLAEIEES
                     YNSGNLSTREYLSDFFDLVSNFPLDFSDSYFDRINTKNSFNDFISYCKESDHELFIVS
                     DFFDLYINKVLTQENISLKYFSGEIINNPDPEKLEIFYPYTDDYCKLCNTCKRNILIS
                     NTNDLENEVSVYIGDGISDQCVSSFADIVFAKGKLASYCWKNNITYFEFDDFSDIINK
                     LSKPENKKFIKQRNEAIVRRRDVLMGG"
     gene            complement(1721..>3656)
                     /locus_tag="PLX80_13920"
     CDS             complement(1721..>3656)
                     /locus_tag="PLX80_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21934.1"
                     /translation="NILIDKLDQNQKGKVTFILNDLLNFKTFEYFNFIEEKPDFIKAK
                     GVFGFADEFVKCNNDKHLRLMRYILDEYIIVDNFKTAELLSDDNYYKFITLEGDIVTK
                     GFIRSGGKVNEESVRLGRQKVIDAIEISLESIKDNVSVVKEEMSSLNIEFESVPVEVQ
                     SKISTDLRNEITRLQNEISKLEFKNEQINKSETANESSYQSISESNKELSKNIDLQIA
                     ELNKVENEQFNIDKELLFITEEFNEIEKNFSQESSDYNNFNLEFTKLKNEYKNEEQNK
                     IRIINTISFNEKQIKINTDNIVENNEKLKILSETLESNRSRLKILTEELSVINRRLED
                     EKMLYEDKKTEQHKIDLLQRQNRLDFDAVSQKLINAQVKIKENEIKSGQVKDYIFRKY
                     EKDISINESVQIESFNYEDFDLLISKKEIDTLSERLKKLGGGYQQMLFEDFENEKADL
                     EKLIEQRDDLLESEKDIRKTIDRINKEARERFLTTFELIRENFIRIFKELFSEGDEAN
                     LRIVHEETEEGKKEEDPLEAKIEIIAKPRGKRPTSIELLSGGEKTLTAIALLFAIYLV
                     KPSPFCVLDEVDAPLDVANLGRFNNMIRKFADNTQFILITHNERTMESVDRLYGVTMQ
                     EAGITTIVETKFKTDEKLQA"
BASE COUNT         1185 a          625 c          498 g         1348 t
ORIGIN      
        1 cattattttt tacttgcttt ttttaacgac aaaaaagcaa caaaaaaagc cgccccgagt
       61 accgtggaag ttaacttgcc ggaaaatctt tcgtcccgag tactcgggac tgcaaaaata
      121 taaactcctc aaccgttaga aaagcactaa cggtttcgtc aaacaatata tttttgcttt
      181 acgccactgc tcagaatttc ttatcggcag tttatcacag gcgggtaatt ctaacaaaat
      241 ttgaaacaaa attaaaatgt tagatcagtt ttttaaaaat aacaaattta atatttaatg
      301 aatataaaaa ctaacttatt aataattcaa aatatttaga tgcttctttt ttagatcact
      361 ttgaaacaaa ctttaagcag gtagtaaata attattatag cggaagtaat taagaatgcc
      421 tgaagagaac gtctttcaga tttgaatccg cttttgacat tcagttcaag atcagaaatt
      481 atgtgtgaag gcagtttctt aaattttgga aaaaacctgc ctacatattt tacatagtcg
      541 gaataaacat tcgtgaattt tgactgtagg aattcttctt cggcaagtac tatacagtga
      601 tactgaaaag caaaaaagaa taaggcaaat atctgcagat atggaaataa agcattgctc
      661 attattccaa tacctatgta gatcattatg tttccaaggt ataatggatt tcgtatgaca
      721 gaataaggac cctgcgttac aagataagtc ccgccgactc ctgaagtagt acgagtttca
      781 ctgcctgcaa agctgactga ccaaattcgt ataatctctc ctgttaaagc aataataaat
      841 ccaatgatga tagtgtaaag tgtcggctcc ataaataata tcatagtgaa aacaaatggt
      901 aacggaatgt aacttctgta tttaaaaaat atgctgccta aatttttcat gataaatata
      961 tttttataaa ttatttattt gtacagttct taagaaaatt ttttaaccgc ccatcagaac
     1021 atccctgcgt ctgactattg cttcatttct ttgtttgata aattttttat tctccggctt
     1081 actgagctta ttaataatat cactgaaatc atcaaattca aaataagtaa tattattttt
     1141 ccagcaatat gaagcaagtt taccttttgc aaatacaata tctgcaaagc tgcttacaca
     1201 ctggtcagaa ataccgtcac cgatataaac tgatacttca ttttcaagat catttgtatt
     1261 gctgatcaga atatttctct tgcatgtatt gcaaagctta caataatcat ctgtataggg
     1321 ataaaaaatt tctaattttt cagggtcagg attattaatt atttcccctg aaaaatattt
     1381 taaactaata ttttcctgtg ttaaaacttt gttaatgtat agatcaaaaa aatcactaac
     1441 tataaaaagc tcatggtcac tttctttaca gtatgaaatg aagtcattaa atgagttttt
     1501 tgtatttatc cgatcaaaat aagagtcaga aaaatctaaa ggaaaattac taacaagatc
     1561 aaaaaaatca gataagtatt cacgtgtact taagtttcca gagttataac tttcttctat
     1621 ctcagcgagt cttttgagtt tattttcagg gatgaaattt tttaaagaat ttataaccga
     1681 atcactgtct gataatatta ttctgaattc gcaaaatatc ttaagcctgt aacttttcat
     1741 cagtcttaaa ttttgtctcg actatagttg taataccggc ttcctgcata gtcaccccat
     1801 aaagtctgtc aacagattcc atagttcttt cgttatgagt gatcaaaatg aactgagtat
     1861 tatctgcgaa ttttctgatc atattattaa atcttcccag attagcaacg tcaagcggag
     1921 catctacttc atctaaaaca caaaatggtg aaggctttac aagataaata gcaaacagaa
     1981 gagcgatcgc agtaagtgtt ttttcccctc ccgacaataa ctcaattgac gtaggtcgtt
     2041 ttcctctggg tttagcaatt atctcaatct ttgcttcaag aggatcttct tcttttttac
     2101 cttcttcagt ttcttcatgc acgatcctga gatttgcttc atctccttct gaaaaaagct
     2161 ctttaaaaat tcggataaaa ttttccctga tgagttcaaa tgtagttaga aatctttctc
     2221 gggcttcttt attaattcgg tcaatagttt ttctgatatc tttctctgat tcaagaagat
     2281 catctctttg ctcgatcagt ttttcaagat ctgctttttc attttcaaaa tcttcaaaaa
     2341 gcatttgctg atatccgcct ccaagctttt tcagtctttc gctcagagta tcaatctctt
     2401 tttttgaaat taaaagatca aagtcttcat aattaaaaga ttcaatttga acactttcat
     2461 taatagaaat atccttttca tattttctga aaatataatc ttttacctgt ccggatttaa
     2521 tttcattctc tttgatcttt acctgtgcat ttataagctt ttgtgaaaca gcatcaaagt
     2581 caagcctgtt ctgtctttga agtaagtcga tcttatgctg ttcagttttt ttatcctcat
     2641 ataacatttt ctcatcttca agacgtctgt taatgacaga taattcctca gttaatattt
     2701 taagtctgct cctgttgctc tctaatgttt cactcagaat cttaagcttt tcattattct
     2761 caacaatatt atcagtattt atttttatct gcttttcatt aaatgaaata gtgttgataa
     2821 tacgaatttt attttgctct tcatttttat attcattttt cagttttgta aattcaagat
     2881 tgaaattatt ataatctgaa gattcctgtg aaaagttctt ttcaatttca ttgaattctt
     2941 cagtaataaa gagaagttct ttatctatat taaattgttc attctcaact ttattaagtt
     3001 cagcgatctg cagatctata tttttactga gttccttatt ggattcggaa atagattgat
     3061 aagaagattc atttgctgtt tcagacttat taatttgttc attcttaaat tcaagctttg
     3121 agatctcatt ctgaagccgg gtaatttcat ttcttagatc tgtgctgatc ttactttgta
     3181 cttcaacagg tacagattca aattcaatat tgagggaaga catctcttct tttacaacac
     3241 tgacattatc tttgatcgat tctaatgaaa tttcaatagc gtctataact ttttgtctgc
     3301 caagtcttac agattcttca ttcactttac cacccgacct gatgaaacct tttgtaacaa
     3361 tgtcaccttc gagagtaatg aatttataat aattatcatc agaaagtaat tcagcagttt
     3421 tgaaattatc tacaataata tattcatcaa gaatatatct cataagtctg agatgtttgt
     3481 cattattgca ttttacaaat tcatcagcaa atccaaacac acctttagct tttataaaat
     3541 caggcttttc ctctataaag ttaaaatatt caaaggtctt aaaattcagc agatcattta
     3601 agatgaacgt tactttacct ttttgatttt gatcaagttt atcaattaat atattc
//
LOCUS       DAORVQ010000271         3648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20752_length_3648_cov_5.986641, whole genome shotgun sequence.
ACCESSION   DAORVQ010000271 DAORVQ010000000
VERSION     DAORVQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20752_length_3648_cov_5.986641"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1072
                     /locus_tag="PLX80_13925"
     CDS             134..1072
                     /locus_tag="PLX80_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQY21935.1"
                     /translation="MKILVKFILTFAIIFLTCVYSVKSQSYISLSFDDPNVTSDEVAG
                     YEAINDSLLLKLKRNNLTAALFVCGKRVDSPEGNALINSWNEQGHSISNHSYDHRNFG
                     SSKMSFNDFKNDFEKNLPLIESQSNYKKRYRFPYLKEGNTKEKIDSCREFLKSLGYTN
                     GYVSIDASDWYIDQVLRDSLKANPETDLKGFKEFYIYHILERSRFYDSLAAELTGRKI
                     KHNLLLHYNFINALFLEDLIIALKENNFVLINTEDAYTDKVYESLPNIIPAGESIIWS
                     LAKESGRYNDILRYPAEDSPYEEEKLKYFLNKYSVK"
     gene            complement(1501..2709)
                     /locus_tag="PLX80_13930"
     CDS             complement(1501..2709)
                     /locus_tag="PLX80_13930"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQY21936.1"
                     /translation="MKTLNKVISVFCIMILFGLTSEISAQDKGAVYGVSTLGDNSKIV
                     DVDIPESLNPGEEYSASITFINTGKNIWDKDDNYLLSLYDQSDNLYKSDVWGIKNVNI
                     PNNVTPGEKAIVNFKIKAPYEPGVYNSKWAMTKDNNYFGEYSINVTKVNGEGVPVVYS
                     VEGYESRFISVSLPENMNAGEKYKVSVTIENTGMYPWNSSEGYTLAPVQQSAAKVYSD
                     WNSTPVALSSTIESGQTSSIEFYVTAPPSPGFYEMQWMMKKGDSYFGQASDNVTVNVS
                     GIIAIKELPPGTNNSSYIKQQIPNSVSFNEELDVFVTMANTGNTAWIKGNEQLVVVDK
                     DLKIQSVNPWGIGYIQLPEDIPPGKVVTFNFKVKPSEIGSQHFQMMMMDKDGNLFGGP
                     SESVQVIVRN"
     gene            complement(3030..3467)
                     /locus_tag="PLX80_13935"
     CDS             complement(3030..3467)
                     /locus_tag="PLX80_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY21937.1"
                     /translation="MARTSTYLNFARNTEEAFNFYRSVFGGEFTGGIYRFKDIPASDD
                     MPPMPEDDKQLVMHVELAIIGGHMLMGTDAPESMGFNLKFGNNFSINLEPDTRAETEK
                     LFKALSEGGKITTELQEMFWGAYFGSCTDKFGVQWMFNCPEKA"
BASE COUNT         1222 a          613 c          527 g         1286 t
ORIGIN      
        1 aagtttcaaa gttgatctta aatctattgt atagcttaat cagacttttt gatttccaac
       61 atcgcacgaa aatctttttt gaacacaaac aacgaagcca gttttagatt caattattaa
      121 acttaatcta taaatgaaaa ttttagtaaa atttatttta acatttgcaa taatattttt
      181 gacctgtgta tattcagtca aaagccagtc atacatttca ttaagctttg atgatccgaa
      241 tgttacatct gatgaagtag caggatatga agcaattaat gacagtcttt tgcttaagct
      301 caaaagaaat aatctgactg ctgctttatt tgtttgcgga aaaagagttg attcacctga
      361 aggaaatgca cttataaatt catggaatga acaaggacac tccatttcaa atcattcata
      421 tgatcatcgt aatttcggtt catcaaagat gagttttaat gatttcaaaa atgattttga
      481 aaaaaattta cctttgatag aatcgcagtc taattataaa aaaagataca gatttccgta
      541 tttaaaggaa ggtaatacaa aagaaaaaat tgactcatgc agagagtttt taaaaagtct
      601 cggttatact aacggatatg tttcgatcga tgcatctgac tggtatatag atcaggtatt
      661 gagggattct cttaaagcta atccagagac agatctcaaa ggttttaaag aattttatat
      721 ttatcatatc cttgagagaa gcagatttta tgacagcctg gctgctgaat taacgggcag
      781 aaaaataaag cataatttgc ttttacatta caatttcata aatgctttat tcctcgaaga
      841 tttaataatt gctttgaagg aaaataattt tgttttgata aacacagaag acgcatacac
      901 cgataaagtt tatgaaagtc ttcccaatat tatccccgca ggtgaaagta taatctggtc
      961 acttgcaaaa gagtccggca gatataatga tattctccgc tatcctgccg aagactctcc
     1021 ttatgaagaa gaaaagctta aatatttttt gaataaatat tctgttaaat aattttttat
     1081 ttatatataa agtcatgtcc atcccagacg tttcattgca aaattccagt ttcattattt
     1141 tctacttact tttttataac gactgtatta cagacgggat tttttaaaac aaaaaacttc
     1201 tatgattatt tttgctgcat tgcaagaagc tgctttaata aaaaagatta attatttaaa
     1261 atagaagtat ctcaaaacat tattcctttt taaatatttt ggacggtact aatacaaagt
     1321 atattttcga ctttttttta agcagttgga aaaacaatta gagagcttaa ttttttgttg
     1381 tcaccagtat taaaaaacga aggtttggaa atattatcaa ttcccaaacc attcgattta
     1441 tattattatt ttttaattac aattaataat aattctattt aacagactac ctgttttttt
     1501 ttaatttctt acaattacct gaacagattc agatggacca ccaaacaaat taccatcttt
     1561 atccatcatc atcatttgaa aatgctgtga tccgatttca gatggtttta ctttaaaatt
     1621 aaaagttaca actttaccgg gtggtatatc ttctggaagc tgaatatatc caattcccca
     1681 cggatttact gattgtatct ttagatcttt gtctaccaca acaagttgtt catttccttt
     1741 tatccatgct gtgtttccgg tattagccat tgtcacaaat acatccagtt cttcattgaa
     1801 agaaactgaa tttggaattt gctgcttaat gtaacttgag ttatttgttc ccggcggcaa
     1861 ttcctttatg gcaataattc ctgatacatt cactgtaaca ttatcagatg cctgaccaaa
     1921 atatgaatct ccttttttca tcatccattg catttcataa aatcctggag acggcggcgc
     1981 tgtaacataa aattctatag atgatgtctg accgctttct attgtacttg ataatgcaac
     2041 tggtgtagaa ttccagtctg agtatacttt agcagctgat tgctggactg gggcaagtgt
     2101 gtaaccttcc gatgaattcc atggatacat cccagtgttt tcaatagtta cagacacttt
     2161 atatttctct cctgcattca tattttccgg taaagaaacg ctgataaatc tggattcata
     2221 accttctact gaatatacta ccggaactcc ttcaccattt acctttgtca cgttgataga
     2281 atattcacca aaataattat tatccttagt cattgcccat tttgaattat atactccggg
     2341 ttcataaggt gcttttattt taaagttaac aattgctttt tcacccggtg taacattgtt
     2401 cggaatatta acattcttaa ttccccatac atcagattta tataaattat cagactgatc
     2461 atacagactc aataaataat tgtcatcctt gtcccatata tttttacctg tattgataaa
     2521 agtaatagat gctgaatatt cttcacccgg attcagactt tcaggaatgt caacatctac
     2581 tattttagaa ttatcaccga gtgtagaaac tccgtaaaca gctcctttgt cctgtgctga
     2641 gatttctgac gttaatccaa acaaaatcat tatacaaaaa actgatatta ctttattaag
     2701 ggttttcata attttatatt aatttataaa aatttgtaat ttaaagttat tcattgaaac
     2761 ttcaattggg ataactttaa aacaatatta agcaatttgt tattgtataa tagtgcaatg
     2821 cccgtgccaa atagaaatgt aaagaataaa gactgttttg attgataata tgccgggatt
     2881 atttattgat ctgacaattt tgacacataa ttgtcataaa taaaaatcag taaaaaaaaa
     2941 agaaatgcag gaaaggcaga atcggtttaa taatattaag agattctgcc ttttagtaaa
     3001 tctgagaata taagataagg aaaattttat tacgctttct ccgggcagtt aaacatccat
     3061 tgtacaccga acttatcagt acagcttcca aagtatgcac cccagaacat ttcctgaagc
     3121 tccgttgtaa tttttccacc ttcggataaa gctttaaaca atttttcggt ttctgcgcgg
     3181 gtatccggtt caaggttaat cgaaaaatta tttccaaatt tcagattaaa acccattgac
     3241 tcaggagcat cagttcccat aagcatatgt ccgccgatta ttgcgagttc aacatgcatt
     3301 acaagttgtt tatcgtcttc cggcattggc ggcatatcat cagatgcagg tatgtcttta
     3361 aatctgtaaa tgcctccggt aaattcgccg ccaaagactg atctgtaaaa attaaatgct
     3421 tcttctgtat ttcttgcaaa attcagataa gtgcttgttc gtgccatgat agttatttta
     3481 attttaaaaa agaaataata taacttttaa taatttattt caatacttaa ttcggattta
     3541 attttttaat taaattacac gacacaaaat cccgaacttt ccccaaacca aagaaataat
     3601 ttcaaaaact ttaattccgc tttattttca ttgaatatat tttttccc
//
LOCUS       DAORVQ010000272         3642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20811_length_3642_cov_4.484249, whole genome shotgun sequence.
ACCESSION   DAORVQ010000272 DAORVQ010000000
VERSION     DAORVQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_3642_cov_4.484249"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..3268)
                     /locus_tag="PLX80_13940"
     CDS             complement(245..3268)
                     /locus_tag="PLX80_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY21938.1"
                     /translation="MKNNPSGNVTFLFTDIESSTQLAQDFPETHQLTLEIHNKILTEA
                     IESNSGFIFKIIGDAYCAAFENASDAVKAAVIIQTGLKNEEWKDAVIKVRIGIHSGQA
                     EWIENNYMGYITLARTARVMSSSYGEQIIISVDVFNKLSDSELTNSNYSIRDLGERRL
                     KDVIKPIRLYQVMGPGLREEFPPLKTLDARPNNLPLQLTNFIGRENELNQVKNIFADS
                     HLLTITGSGGSGKSRLALQTGADLIDDFANGVWLAELAPVSEPELIPQKIIEEIGIKE
                     EPNESPEKTLKDYLKNKEILIILDNCEHLIDACAALAENLLTYCPKLKILATSREALK
                     CRGEHLFRIPSLSHPESDSDETPEQLNQYESVRLFIERAISLNPAFRVNKDNAPALAA
                     ICSRLDGIPLAIELAAARTKILTLEKIHERLDNRFSLLTGGKRTALPRQQTLRALIDY
                     SYDLLENDEKILWSRLSVFSGGWTLEEAEEICSDDEVDKDDVIDLLSRLSEKSIILFD
                     EINDRYNILETIKQYGKEKLTGSEFILSNHLLYFKNLTGSAAPELRGKNSKHWLDKLE
                     SEYHNIKSAAEWSLDGGEIQDGVSLLNSLIEFWRIRGHYFTESLLLETFLANRKKLDK
                     SSLASVLITAGSISNIQGNLEYAQKLFDESLSLSKELNEKSKIASSISSMGFIESAQG
                     KYEKARILFEESLALHRELGNTQKIATDLNNLGVILCDIGDIGNFDQSKMYLEECLTL
                     LRKMGDNQGLARSLGNLAILSNIKGDYISAKNLYEESLEMLREIGDKRGISRTLNYLS
                     IVARNLGDYDEERKYIEECYKIISLTDDKRGTAIALNNLGETELIQGNFKKAKQYIEE
                     SLEISKENGYKNLIARSLNYLGTVTHRLGDNDLAMKLFKESLQMSIELDDKYSTAEHL
                     NNFAGMLSEELNYLFEIISIFSAIDITIQSMGIVFERFEQTVHDNAIRNLREKFRDKD
                     FEEFNAHWEKGKKMTLEEACELAMRLPQRHQDTKT"
BASE COUNT         1097 a          704 c          618 g         1223 t
ORIGIN      
        1 attcttactg cttgttccag tgctcccgcg ataggtagag atttgaagaa gaatttaatt
       61 gtaatcgatt cagcgataat tattaaaccg taatgtgatt aaaaattttc aaacctcgca
      121 gaggtgacat ttttgtagaa atgcgattaa gataattatc aaactccgta aaaggataca
      181 ttattgtaga agtgctaaca atgaaagaaa ccgaaagctc aggaataaac acatttttaa
      241 aactttaagt cttagtgtcc tggtgtcttt gtggtaaccg catcgccaac tcacacgcct
      301 cttccaatgt catcttcttt cccttttccc agtgagcatt aaattcttca aagtctttgt
      361 cacggaattt ttcacggagg tttctgatag cattgtcgtg aacggtttgt tcaaatcttt
      421 caaacactat tcccattgac tgaattgtta tatcgattgc tgaaaatatt gagattattt
      481 cgaaaagata atttaattcc tctgaaagca taccggcaaa attattaaga tgttcggctg
      541 tgctgtattt atcatcaagt tcaatgctca tttgcagact ctctttaaat aatttcattg
      601 caagatcatt atctccaagt ctgtgagtga cagtgccaag ataattcagt gaccttgcaa
      661 tcaaattttt gtatccgttc tcttttgaga tctcaagact ttcttcaata tattgttttg
      721 cttttttaaa attaccctga atgagttctg tctcaccaag attatttaag gcaattgcag
      781 ttcctctttt atcatctgtt aaagaaataa ttttgtaaca ttcttctata tactttcttt
      841 cctcatcata atcacccaaa ttgcgggcaa cgatactgag ataattcaat gtcctggata
      901 ttcctctttt atcaccgatc tcccgcagca tctccagact ttcttcatat aaatttttag
      961 cagaaatata atcacctttg atatttgaaa gtatggcaag attaccaaga gacctggcaa
     1021 gcccttgatt atcgcccatt tttctgagca gtgtaagaca ttcctccaga tacattttcg
     1081 actgatcaaa attaccaata tcaccaatat cacaaagtat tactccaaga ttgttcagat
     1141 cagtggcaat cttttgagtg ttcccaagtt cacggtgcaa tgcaagactt tcttcaaata
     1201 agatccttgc tttttcatat ttaccctgtg cactctctat aaaacccata gaactaatgg
     1261 aagaagcaat ctttgatttt tcatttagtt cttttgacaa agaaagactt tcatcaaata
     1321 atttctgtgc atattccaga ttcccctgaa tgttggatat tgaaccggca gtaataagaa
     1381 cagaagcaag agaagacttg tctaactttt ttctgtttgc taaaaatgtt tccagcaaaa
     1441 gactttccgt aaaataatgt ccgcggatcc tccagaattc aattaaagaa ttcaagagtg
     1501 aaacaccatc ctgaatttct ccgccgtcaa gcgaccattc ggcagcagac ttgatgttat
     1561 gatattctga ctcgagttta tccaaccagt gtttagaatt ttttcctcta agctccggtg
     1621 cagcagaacc tgtaagattc ttaaagtaaa gcagatgatt agataaaata aattctgatc
     1681 cggtgagttt ctcttttcca tattgcttaa tagtttcaag gatgttatat ctgtcattta
     1741 tttcgtcaaa taatattatt gatttttcgg aaagtctgct gagcagatct ataacatcat
     1801 ccttatccac ttcatcatcc gaacaaattt cttcagcttc ctccaaagtc cagcctccgg
     1861 aaaaaactga aagtctgctc cataatattt tttcatcatt ttccaaaaga tcataactgt
     1921 aatcgatcaa agctcttaaa gtctgctgtc ttggcaatgc agttctctta ccgccggtaa
     1981 gcagactgaa tctgttatca agtctttcat gtattttttc taaagttaaa atttttgttc
     2041 gggctgctgc aagttctata gccaatggaa tcccgtcaag cctggagcaa atagcagcaa
     2101 gtgcaggagc gttatcttta ttcactctga atgcgggatt aagtgaaatt gctctttcaa
     2161 taaacagccg gactgactca tattgattga gctgttcggg agtttcatct gaatctgatt
     2221 caggatgtga cagcgaagga attctgaata aatgctcacc tctgcatttt aaagcttcac
     2281 ggctggtcgc taagattttc agcttcggac agtatgtaag taaattttct gcgagtgctg
     2341 cacatgcatc aattaaatgt tcacagttat caagtatgat aagaatttct ttgtttttca
     2401 gataatcttt taatgttttt tcaggtgatt catttggttc ttctttaatg ccgatctctt
     2461 caatgatctt ctgaggtatt aactcaggtt ctgataccgg tgccagttct gccagccata
     2521 caccatttgc aaaatcatcg atcagatccg cacctgtttg taaagctaat ctcgattttc
     2581 ctgaaccgcc tgagccggta atggttaata aatgtgaatc agcaaatata ttttttacct
     2641 gattcagttc attctccctg ccgataaaat tcgtaagctg cagcggaaga ttatttggtc
     2701 tggcatcgag tgtttttaat ggaggaaatt cttctctgag accgggcccc atcacctgat
     2761 aaagtctgat aggtttaata acatctttta gtctcctctc accaagatca cgaatagaat
     2821 aattgctgtt ggttaattcc gaatctgaaa gtttattaaa cacatcaaca gaaattatga
     2881 tctgttcacc ataagaagaa gacatcactc ttgcagttct ggcaagggta atatagccca
     2941 tataattatt ttcaatccat tcagcctgac cgctgtgaat accaattcta actttgatca
     3001 ccgcatcttt ccattcctca tttttaagcc ctgtctgtat aatgacggca gcttttacag
     3061 catcacttgc attttcaaat gcagcacaat atgcatcacc aatgatcttg aatataaatc
     3121 cgctgttcga ttcgattgct tcagttaaaa ttttattatg tatctccaga gtaagctgat
     3181 gagtttccgg aaaatcctga gcgagctgtg tactgctttc gatgtctgta aataaaaaag
     3241 ttacatttcc tgagggatta tttttcatga aacattgtac acaaatattg aaaataattt
     3301 atcctgatat atctcatatt ttttagatct attaataaat gttatagaaa gatttgtatg
     3361 taattttgaa aatgtaaatt acaaaatata gcggaaagaa aaatgtgatt tagagaatta
     3421 gtggaaataa aattcccggt agtgagttta aaagagagct tcagattcaa gttagttcag
     3481 aaaccggagt tttggaacga gatgaacgag tgaaaaacag atgtttgaga aagacaggaa
     3541 acaaaaattc aaaactgtca ctgcgaatcg tgacggcttt tttttgtctg attcccggat
     3601 tactttgatt acctgcccaa cgatggcgag attgccgccg tg
//
LOCUS       DAORVQ010000273         3634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_20886_length_3634_cov_5.776194, whole genome shotgun sequence.
ACCESSION   DAORVQ010000273 DAORVQ010000000
VERSION     DAORVQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20886_length_3634_cov_5.776194"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..3263
                     /locus_tag="PLX80_13945"
     CDS             57..3263
                     /locus_tag="PLX80_13945"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HQY21939.1"
                     /translation="MSYLYIDTDNAPSEESGIARERDYLINEIDFQPAEKIFHPVNPS
                     DYIYRIQDVPHIKEWLSTTGEIAKLGTMNTGAGQIRPAARLAFFHNYDEIVSKLTAAK
                     SRITDSKSINIVKENHKIKNVNTDKINVYVVTSLSGGTGSGMILDMGFLIRSIFRNQA
                     VSTAYVVLPKIFQSYGKERVFANGYAALKELEHYNLMNTFEVSWKKNEPHKFQPGVFD
                     DTYIIDGENAKNLSLSDQNNRDIYKMLADTIFQDFSNSDFANYKRGVRVNLVQYKQRL
                     WPDDNSVTDNTYSRKYSALGQATISIPVDRIIISCAYKLCEEIINYYLTYAEGSQSEV
                     DGYLINEFLPELGLLESKNKFQLLDSLYKSGEKTSLQSNIKTFLTTIQNDLVSGKRGS
                     TWSSYLLNEKMKFDTNFKDDNDVNRQGMFFSQIHSNRTKDIQNLIGDRERNTIGQLER
                     KINALVNDSTRGVFYAINLLHRLQFILTDGNFDYIPKYEKEIKDLQSLATKLDAEFRR
                     RFQELKEDETRTKFNVLKKSAMSGSLEKVLNVMEDYYNTLIKLKSRYYAREICLRILS
                     LIETKEKTANDRIISTGLIPDLNKLTGNLGTLKNNFRQKFNHFIQKQETSFNLMVYEP
                     DEVSNEYYVKYIGTGQTAFENIKNLSNKLLKDLGASDVTDIVNILRERDAKLIESKML
                     SFARKQFENIREDYNITDILFEKDSIRSESKIRGMFNQAFPWMRTREIPGSFKLDESA
                     KKFYIGIKTNTPSFKKFQTLIQSIAGSSVEFKDSADNSSITFYSEWAGFPLFYSYTIA
                     EEMKPYYKQLSQNHNVDLHINKNYFIYNDIIPLNDDEKVRLDEAYKAYLLGLIFGIFD
                     VIPETETSKAIYKYTKQEGITVKRVEQLGVETRLINRLFEEQGKDAMRYRILKDAEAV
                     QDKFFKANKLAEMLAIYEYYYDEIYKPVEYEISGDAKRKVETYQYSILRNLSREIENK
                     ISLKDKDQFVDKVISFKRNPDSYSRLIGDGGKRVLRVNEILSDAERDALTSDKSEITI
                     SDNAGVSKNRLKEFKEALDEGLITNEEFEKAKKEFLKI"
     gene            3265..3630
                     /locus_tag="PLX80_13950"
     CDS             3265..3630
                     /locus_tag="PLX80_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY21940.1"
                     /translation="MPTYDYRCDDCGHFFEEFQSMKDAKLTKCPECGKETLKRLIGAG
                     SGLIFKGTGFYLTDYKNKPAESASSTTKNTTVKDKEKTEPSETKADVKVSSKPTSENT
                     SSKSDTKLTEKKSAPSKTD"
BASE COUNT         1358 a          590 c          688 g          998 t
ORIGIN      
        1 tcaggatcag aagacaattc gtagaaaagt actgatctat aaatgacttc cctattatat
       61 catatcttta tatagatact gacaatgccc cttcggaaga aagcggcatt gccagagaaa
      121 gagattattt aataaatgaa attgattttc agccggcgga aaaaatcttt catccggtaa
      181 atccttcgga ttatatttac agaattcaag acgttcctca tattaaagaa tggctcagca
      241 cgaccggtga gatcgctaag ctggggacta tgaatacagg cgcaggtcag atcagacccg
      301 ctgccagact tgcttttttt cacaattacg atgagatagt ttcaaagctc actgccgcaa
      361 aatcaaggat caccgattcg aaatccatta atatcgttaa agaaaatcat aagataaaaa
      421 atgtaaatac cgataagata aatgtttatg ttgtaacttc tcttagcggc ggtacaggct
      481 ccggaatgat ccttgacatg ggatttctta tcagaagtat tttcagaaat caggcagtat
      541 caacggctta cgtggtgctt ccgaaaattt ttcaatccta cggaaaagaa agagtattcg
      601 caaacggata tgcagctctc aaagaattag agcattacaa tcttatgaat acgtttgagg
      661 tatcatggaa aaaaaatgaa ccgcataaat ttcagccggg tgtatttgac gacacttata
      721 taatagacgg agagaatgca aaaaaccttt ccctctcgga tcagaataac agagacattt
      781 acaaaatgct tgctgacact atctttcagg atttttcaaa ttccgatttt gcaaattaca
      841 aaagaggtgt aagggtaaac cttgttcagt acaagcaaag actctggccg gatgataatt
      901 cagtaacaga caatacatat tcaagaaaat attcagcact tggacaggcg acaatttcaa
      961 tacctgtgga caggataata atttcatgtg catataaatt atgcgaggaa ataataaatt
     1021 attatctgac ctatgccgaa ggttcacaaa gcgaagtcga tggatatctt atcaacgaat
     1081 ttcttcctga gcttggactg cttgaatcca aaaataaatt tcagcttctt gattcactat
     1141 acaaatcagg tgaaaaaacc tcgctgcaat ctaacataaa aactttcctt actactatac
     1201 agaatgacct tgtgagcgga aaacgcggct cgacatggtc ttcttatctt ttgaatgaga
     1261 agatgaagtt tgatacaaac ttcaaagatg ataatgatgt gaacagacag ggaatgttct
     1321 tttcacagat ccattcaaac agaacaaagg atattcagaa tcttataggt gacagggaaa
     1381 gaaatacgat cggtcagctt gagagaaaga tcaatgctct cgtgaatgat tccaccagag
     1441 gagtttttta tgcgatcaat cttcttcaca gattacagtt catattgaca gacggaaatt
     1501 ttgattacat tccaaagtat gaaaaagaaa ttaaagatct tcagtcactt gctacaaaat
     1561 tagatgcaga attcagaaga agatttcagg agcttaaaga agacgagaca agaactaaat
     1621 tcaatgtgct gaaaaagtca gcaatgtcag gcagtctgga aaaagttctg aatgtaatgg
     1681 aagattatta caatactctg atcaaactta agtcaagata ttatgcgcgg gagatatgtc
     1741 tgagaatttt atctttgatt gaaacaaaag aaaagacagc caatgacagg atcatttcga
     1801 ccggacttat ccccgatctg aataaactca caggtaatct cggaacgctt aaaaataatt
     1861 tcagacagaa gtttaatcat tttatacaaa aacaggaaac cagttttaat ttgatggtat
     1921 atgagcccga tgaagtcagc aatgaatatt atgtaaaata tatcggcacg ggacaaactg
     1981 catttgagaa tattaaaaat ctttcgaata aacttttaaa agatcttggc gcatcagatg
     2041 tcacggacat tgtaaatatt ttaagagaaa gagatgcaaa gcttattgaa tcaaagatgc
     2101 tgagctttgc aagaaagcag tttgaaaata tcagagaaga ttacaatatt actgatatat
     2161 tatttgaaaa agattcgatc aggagtgagt ctaaaataag aggaatgttc aatcaggcat
     2221 ttccatggat gcggacgaga gaaattccgg gaagctttaa gctggatgag tcagcaaaga
     2281 aattttacat cgggataaaa acgaatactc cttcattcaa aaaatttcag acactgatac
     2341 aatctattgc aggttcatca gttgaattca aagattccgc tgataattct tccatcacat
     2401 tttattcaga gtgggctgga tttcctttgt tttattctta cacgatcgct gaagagatga
     2461 agccttatta caaacagctt tcacaaaatc acaatgtaga tcttcatatc aataaaaatt
     2521 attttatcta caacgacatt attcctttga atgatgatga gaaagtaaga ttagatgagg
     2581 catacaaagc ttatttgctt ggtttgatct ttggaatatt tgatgttatt cccgaaacag
     2641 aaacttcaaa agccatttac aaatatacaa aacaggaagg tataactgta aaacgtgtag
     2701 agcagcttgg agtcgagacc agacttatca acagattatt tgaagagcag ggtaaagacg
     2761 caatgcgtta taggatactg aaagatgcag aagctgtgca ggataaattt ttcaaagcaa
     2821 acaaactagc agagatgctg gcaatttacg aatattacta tgatgaaatt tacaaacctg
     2881 tagagtatga aatttcagga gatgcaaaaa gaaaagttga gacatatcaa tattccatat
     2941 taagaaattt atcgagagag atcgagaata aaatttcatt aaaagataaa gaccagtttg
     3001 ttgataaagt aatttcattt aaaagaaatc ccgattctta ctccaggtta atcggagatg
     3061 gcgggaagag ggtactcaga gtgaatgaaa ttttatcaga tgcggaaaga gatgctttaa
     3121 cttctgataa atccgaaatt acaatttctg ataatgcagg tgtaagtaaa aacagactaa
     3181 aagaatttaa agaagcatta gatgaaggat tgatcacaaa tgaagaattt gaaaaagcaa
     3241 agaaagaatt tttaaaaata taagatgcca acttacgatt acagatgcga tgactgcggt
     3301 catttctttg aagagtttca gtcgatgaaa gatgcaaagc ttaccaaatg ccctgaatgc
     3361 ggcaaagaaa ctttgaagag acttatcggt gcgggcagcg gattgatttt caaagggaca
     3421 ggtttttatc ttaccgatta taaaaataaa cctgctgaga gtgcatcttc tacaacaaaa
     3481 aatactactg taaaagataa agaaaaaaca gaaccgtctg aaacaaaagc tgacgtcaaa
     3541 gtaagttcaa aaccaacttc cgaaaataca tcatcaaaat ccgatactaa attaacagag
     3601 aagaaatctg ctccttctaa aactgactga tatt
//
LOCUS       DAORVQ010000274         3612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_21108_length_3612_cov_5.869834, whole genome shotgun sequence.
ACCESSION   DAORVQ010000274 DAORVQ010000000
VERSION     DAORVQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21108_length_3612_cov_5.869834"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..437
                     /locus_tag="PLX80_13955"
     CDS             69..437
                     /locus_tag="PLX80_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HQY21941.1"
                     /translation="MDVFIINTSLTISYMSLIVIAYGSILGAISFVRNELGRIKGTYS
                     LKRLNTVKIDFGYYLLLGLDFLIAADVVRTILENTMQDLMILGLSVVIRTLLSYFLGR
                     EITDDEKFREKIDEAQEGKI"
     gene            complement(448..2235)
                     /locus_tag="PLX80_13960"
     CDS             complement(448..2235)
                     /locus_tag="PLX80_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY21942.1"
                     /translation="MKLLYSYLKKYKKLLIIALILAAVNQVFSLLDPFIFRYIIDDYA
                     TKYTEYTTSEFFKGVGFLLMLAVGAALISRIAKNIQDYYLNVITQKLGADIYTDGISH
                     SLKLPYSVFEDQSSGQTLGILQKVRTDSEKMISAFVNVMFTSIVGILFVGIYAFSVHW
                     VIAPIYLSAIPIIGLLSSSFGKKIKTIQKTIVDETTALAGSTTESLRNIELVKSLGLS
                     QQEINRLNNTTDKILLLELKKVKYIRSLSFIQGTIVNLLRTSILFIMMYLIFTQAITT
                     GQFFTLFIYSFYIFGPLQELGNIINIYKEAEVSLNNFQEIMNTPVDPKPKDPVIINEI
                     ETLEFDDVTFRHKSSNTNALSQISFKTGVGKTIAFVGPSGSGKTTLVKLLLGLYRPIE
                     GNILYNGTPFDKVDLDSLREKIGFVSQDSQLFSGTIRENLLFVNPKATDEECYEVLYK
                     SNCHTLLERADNGLDTVIGEGGVKVSGGEKQRLSIARALLRNPNLMIFDEATSALDSI
                     TEEGINKTIREISSGGNHITLMIAHRLSTIMHADTIYVLERGNIIESGKHEELIELKG
                     LYYAMWRQQIGERKFIVESAVLDAEKRMN"
     gene            2478..3545
                     /locus_tag="PLX80_13965"
     CDS             2478..3545
                     /locus_tag="PLX80_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQY21943.1"
                     /translation="MTRLEKIRDRFEPLGVDSFLVKSLPNIRYISGFSGSAANILLTK
                     DKNYFISDFRYKTQSSKEVYKEFDVIIYTQNSMGFLKELSEKNGLKKIGFESNILTYN
                     DYQNLKNEFYELEFIPADSLIENIVSQKNEKEIELTKKAVKITDDTFTELLNIIRPGM
                     TEREVSAEISFLHKIYGADSDSFEPIVASGVRSAFPHARPTDKKIENGELLKLDFGCT
                     VEGMKSDMTRTIAIGEISEECHKIYSIVKEAQLKALDKVKAGVSVKEVDAAARDFITE
                     NGFGENFGHGLGHGLGYDIHERPSLNQRTDYILEKNNIITIEPGIYVEGLCGVRIEDD
                     VIVTEDGCEILNSSSKELITI"
BASE COUNT         1228 a          566 c          646 g         1172 t
ORIGIN      
        1 ttaactatta actattaact attaactatt aactattaac tattaactat taattattaa
       61 ctatttagat ggatgtattt ataattaaca cttcgctgac tattagttat atgagtctga
      121 tcgtaattgc ttatggttca attttaggag ctataagttt tgtcagaaac gaacttggac
      181 ggatcaaagg aacttattct ctcaaacgac tgaacacagt aaaaatagat ttcggatatt
      241 atcttttact cgggctggat tttctaattg ccgcagatgt ggtgaggacc attctcgaaa
      301 acaccatgca ggatctgatg atacttggat taagtgtagt gatcagaact ttactttcct
      361 attttctcgg acgtgaaata actgatgatg aaaagttcag agaaaagatt gatgaagcac
      421 aagagggtaa aatttaaaaa taaaaaatta attcattctt ttttcagcgt caagtactgc
      481 actttcaaca ataaactttc tttcaccgat ctgctgtctc cacattgcat aatacaaacc
      541 ttttaattca ataagttctt cgtgttttcc tgactcgata atatttcctc tttcgagaac
      601 ataaattgta tctgcatgca taatcgtcga aagtctgtgt gcaatcatga gagttatatg
      661 attacctcct gatgaaattt cacggatagt tttatttatt ccttcttcgg taatggagtc
      721 cagtgctgaa gtcgcttcat caaatatcat aagattcgga tttctcagca aagctcttgc
      781 tattgacagc cgctgctttt ctcctccgga aacttttact ccaccttcac ctatgacagt
      841 atcgagacca ttgtcagctc tttcgagaag agtatggcag tttgatttat ataatacttc
      901 atagcattct tcatcggttg ctttgggatt tacgaataat aaattctctc tgatcgtccc
      961 tgaaaaaagc tgagaatcct gagatacaaa tccgatcttt tccctgagac tgtcgagatc
     1021 cactttgtca aaaggagtgc cattatataa aatatttcct tctataggtc tgtataaacc
     1081 aagcaggagt tttaccaatg ttgtctttcc cgagccggac ggaccaacga atgcaattgt
     1141 ttttccaaca cctgtcttaa atgatatctg actcagtgca tttgtatttg aagacttgtg
     1201 tctgaaagtg acatcatcaa attcgagagt ttcaatttca tttataatga caggatcttt
     1261 tggctttgga tcgacaggag tattcattat ttcctgaaaa ttattaagag atacttcagc
     1321 ttctttataa atatttataa tattcccaag ctcctgcagc ggaccgaaaa tataaaatga
     1381 gtaaataaat aaagtaaaga actgacctgt tgtgatcgcc tgcgtaaaga tcagatacat
     1441 cataataaaa agaatactcg ttcgtaataa attaacaata gtaccttgaa taaaactaag
     1501 acttctgatg tattttactt tttttaattc cagcagtaaa attttatcgg tagtattatt
     1561 gagtctgttg atctcctgct gagaaagtcc gagacttttt acgagctcga tatttctaag
     1621 agattcagta gttgatcctg ctaatgctgt agtttcgtca actattgttt tttgaattgt
     1681 cttaattttt tttccgaagg acgaacttaa taaaccaatt atcggaattg cgctaagata
     1741 tatcggagct ataacccagt gaactgagaa agcataaatg cctacaaata aaattccaac
     1801 aatagaagta aacataacat ttacaaaagc tgatatcatt ttttcagaat ctgtccggac
     1861 tttttggaga atgccgagag tctgtccgct gctttgatct tcaaagacag aatagggcag
     1921 cttcagcgaa tgactgatac catctgtata aatgtctgct ccgagctttt gagtaataac
     1981 atttaaataa tagtcctgta tattttttgc gatccttgag atcagtgctg caccgactgc
     2041 aagcataagt aaaaatccaa cgcctttaaa aaattcagaa gttgtatact cggtatattt
     2101 cgttgcatag tcatcgataa tatatctgaa gataaaagga tcaagaagtg aaaacacctg
     2161 attgacagca gcaaggatca atgcaatgat cagaagcttc ttatactttt ttagatatga
     2221 ataaagtaat ttcattttat tttaagagac taaaataaaa ttattaaaat gatatttcaa
     2281 tgttaaagat tggaggaaat aaattgagtt tatgttagtg agcaatattg aaatgcagca
     2341 agaaccttga atatcttaaa gactgaatca gataagaaag ttttatcata taatttgcaa
     2401 gtgttttagc accaaaacat tcattgatat tacctgcatg aatgagtatt tttgaagtta
     2461 ttgtaaaaaa ttaataaatg actcgattag agaaaatcag ggacaggttt gagccgcttg
     2521 gagtggattc atttcttgta aaaagtcttc ctaatatcag atacatttca ggtttttccg
     2581 gaagtgctgc caatatactt ctgactaagg ataaaaatta ttttatttcg gattttagat
     2641 ataagactca atcttcaaaa gaagtttata aagaattcga tgtgataata tatacacaga
     2701 attcgatggg ttttctgaaa gaactttctg aaaagaatgg actaaagaaa attgggtttg
     2761 aatcaaacat acttacatat aatgattatc agaatctcaa aaatgaattt tatgaacttg
     2821 aatttattcc tgcagattca ctgatcgaaa atattgtctc tcaaaaaaat gaaaaggaaa
     2881 ttgagctaac caaaaaagct gtaaagataa ctgatgatac atttactgaa cttcttaata
     2941 tcattagacc cggtatgaca gaacgtgaag tctctgctga aatttctttt cttcataaaa
     3001 tctacggagc tgattcggat tcatttgagc ctatagttgc atctggcgtg agaagtgctt
     3061 ttcctcatgc gagaccgaca gataaaaaaa tcgagaatgg tgaattacta aagttagatt
     3121 ttggatgtac agtcgaaggt atgaaatcag atatgacacg gacaattgca ataggtgaga
     3181 taagtgaaga atgtcataag atctacagca ttgttaaaga ggcacagctt aaagcacttg
     3241 ataaagtaaa ggcaggcgtc agtgtaaaag aagtcgatgc agccgcaagg gattttataa
     3301 ctgaaaacgg atttggcgag aattttggtc acggactcgg tcacggactt ggatatgata
     3361 ttcatgaaag accttcattg aatcagagga cagattacat tcttgaaaaa aataatatta
     3421 tcacaataga gccgggtatt tatgtggaag ggctgtgcgg agtcaggata gaagatgatg
     3481 tgattgttac agaagacgga tgtgaaattt tgaacagttc atcaaaagag ctgatcacaa
     3541 tatagattta atatgagtta ttttaaaaag taatttcatt ttattataaa agatttgata
     3601 tataaaaaga at
//
LOCUS       DAORVQ010000275         3610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_21140_length_3610_cov_4.201969, whole genome shotgun sequence.
ACCESSION   DAORVQ010000275 DAORVQ010000000
VERSION     DAORVQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21140_length_3610_cov_4.201969"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /gene="arsB"
                     /locus_tag="PLX80_13970"
     CDS             <1..974
                     /gene="arsB"
                     /locus_tag="PLX80_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQY21944.1"
                     /translation="GFWNSFQSGTTNIPIAIGLILMMYPPLAKVRYEDLGKVFRNYKI
                     LSLSLVQNWIIGPMLMFFLAVLFLQNYPEYMVGLILIGLARCIAMVIVWNELAKGDTE
                     YAAGLVAFNSIFQVLFFSVYAYIFITVLPPLFGLTGFTVNITIGQIAESVFIYLGIPF
                     LAGIITRFLLLKIKGKEWYQTKFIPRISPVTLIALLFTIFVMFSLKGEMIIKIPFDVV
                     RIAIPLVLYFVIMFFVSFFMGKKIGADYSKTATLSFTAASNNFELAIAVAVAVFGINS
                     GEAFAAVIGPLVEVPVLISLVNISLRLQKKYFKTVEESNVKSPDLCP"
     gene            complement(1317..1751)
                     /locus_tag="PLX80_13975"
     CDS             complement(1317..1751)
                     /locus_tag="PLX80_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQY21945.1"
                     /translation="MKFKTKILQFGNNTGIEISEDLLEKLNGGKKPLVVVTLNKYSYR
                     SAVGKMGDRFMISLSSENRKNAGVNGGDSLEVQIELDTAPRTVDVPDELQTALNKNKT
                     IKDAFEKLAPSKKKAMVMSVIDAKAEETRKKRIEKIISTLSQ"
     gene            complement(2020..3321)
                     /locus_tag="PLX80_13980"
     CDS             complement(2020..3321)
                     /locus_tag="PLX80_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21946.1"
                     /translation="MRNPEKSKYTEISIYYLFRSFIPIFLLFHLYVNVNAQTGWVHQN
                     SGTTLNLFSIRFLNETTGWAVGDSGLILKTTDAGNNWVSRRFGNTLLRALHFIDGNTG
                     VIVGGNYPPPMWGSPTRIIIRTTNSGVNWNTIVNTNETYFKNVQFVNDSVGFIGFNGV
                     YKSTDKGNNWEHYGNIVNQNGFFINENTGYAANGYISTIQKSTNGGIDWINVLKPGKY
                     NVDGGIYFCNQNTGFVTGASGIFKTTDAGSGWQDMWYDDFYFRDVDGIFFSDPEHGVI
                     LAYQNFPNSYIIQSTTNGGNTWNINYSPSRLRSVFFLNNMTGWIAGDQGKILKTTTGG
                     LTIIVQSSNPVPDSYLLDQNFPNPFNPSTNIKFEIPSSVYVTIKVYTILGKEVATLVN
                     EFINEGTYQIEFNGSNLESGIYFYRIQAGDFTDEKSMIILK"
BASE COUNT         1098 a          632 c          561 g         1319 t
ORIGIN      
        1 caggattctg gaattctttc caatcaggaa caacaaatat accaattgca attggtttga
       61 tacttatgat gtatcctcct cttgctaagg taagatatga agaccttggt aaagtattca
      121 gaaattacaa aatactctcg ctttctctgg tacagaactg gataattggt ccgatgctga
      181 tgttttttct tgcggtatta ttcctgcaga actatccgga atacatggta ggtcttattt
      241 taatcggact tgcaagatgt attgccatgg ttattgtatg gaatgaactt gcaaagggag
      301 atactgaata tgcagcaggt ctcgttgctt tcaattctat ttttcaggta ttattctttt
      361 cggtttatgc ttatatattt attactgttt taccgccttt atttggttta acaggtttca
      421 ctgtaaacat aacaatagga cagatagctg aaagtgtttt catatacctc ggcattcctt
      481 tccttgccgg gattattacc agatttttac tattaaaaat aaaagggaaa gaatggtatc
      541 aaactaagtt tattcccagg attagtccgg ttactttaat tgcactcctt ttcacgatct
      601 ttgtcatgtt ttcattaaaa ggtgaaatga taataaaaat cccctttgat gtagtaagga
      661 tagcaattcc gctggtttta tattttgtga ttatgttttt tgtttcattt tttatgggaa
      721 agaagatcgg ggcggattat tcaaaaactg ctacattatc atttactgct gcaagtaata
      781 attttgaact tgcaattgca gtagcggttg ctgttttcgg aattaattcc ggtgaagcat
      841 tcgcagctgt gataggtcct ttggtagaag tacctgtgct gatcagtttg gttaatatat
      901 cgctgaggtt acagaaaaaa tattttaaaa cagtcgaaga aagtaatgtg aaatcccctg
      961 atctgtgtcc ttaaaaaata tttcaatgtc tgttcattat cgtgtcccgt cggcaagaag
     1021 tatgctctct ccgagaatat ggttccgcca cacctgtcga aaattgtctg acggcagaac
     1081 atcaactgca ttctgcctgt aaagtccata cttaccggtt acagaaaaat ataatgaact
     1141 tttatcgtta acgtcttttt cctgtttgga atttttttta atattatatt cccgttcggt
     1201 gtattttact tcgatcccga tgattccttt tgtaccgtct gtatttgtgt atccaatgtc
     1261 tgagcggctg agggatataa atgaatatat cgaaagtgta ttgagataaa actcttctat
     1321 tgacttaatg ttgaaattat tttttctatc cttttttttc tagtctcctc tgctttagca
     1381 tcaatgactg acattaccat tgcctttttt ttgcttggag caagtttttc aaatgcatct
     1441 tttatagttt tatttttatt cagggcagtc tgaagttcat cgggaacatc aactgtcctc
     1501 ggcgcagtat caagttctat ctgtacttca aggctatcac cgccatttac tccggcattc
     1561 tttctgtttt ccgaactgag acttatcatg aacctgtctc ccatttttcc gacagcgctt
     1621 ctgtaagaat atttattcaa tgtcaccact accagcggtt tcttaccgcc gtttaatttt
     1681 tcaagcagat cttcactgat ctcaatgcca gtgttgttcc cgaactggag gatctttgtt
     1741 ttgaatttca tttttactta tttgaatttt tataaattct gaatttcatc taacacaatt
     1801 ttttggatga atggattcga atttggattt aacaaaatta aaaaaattaa aagaatgaat
     1861 caatttgatt ttagtttgag ttcattcaag tattaaaaat ctgtattatt taaaataaaa
     1921 aaaaccggca cgaaataaat catgacggct ttagaataaa tccgtatcct actctctcac
     1981 aaagcatatc attaagtaat acattctcac ctatatatcc tacttcagta ttatcattga
     2041 tttttcatcc gtaaaatccc ctgcttgaat tctataaaaa tatatcccac tttctagatt
     2101 acttccatta aattcaattt gataggttcc ctcatttata aattcattaa caagagtagc
     2161 cacttctttt ccaagtatag tgtatacttt tatggtaaca tatactgatg aaggtatttc
     2221 aaatttaata tttgtgcttg gattaaatgg atttggaaaa ttttgatcaa gcaaataact
     2281 atcaggaact ggatttgaag attggacaat aatagttaat cctccagtag tagttttaag
     2341 tattttccct tgatctcctg ctatccagcc cgtcatatta ttgagaaaaa aaacagacct
     2401 gagcctgctt ggagaataat taatattcca tgtgtttcca ccgtttgttg ttgactgaat
     2461 aatataagag ttaggaaaat tttgatatgc aagaattact ccatgttcag gatcggagaa
     2521 aaaaataccg tcgacatctc tgaaataaaa atcatcatac cacatatcct gccatcctga
     2581 tccagcatcc gttgttttaa aaattcctga tgcgcctgtt acaaatccag tattttgatt
     2641 gcaaaaataa ataccaccgt caacgttata ctttccaggt ttaagtacat ttatccaatc
     2701 aattcctcca tttgttgatt tttgaattgt agagatatac ccgtttgcag catatccggt
     2761 attttcattt ataaaaaatc cattttgatt aacaatattt ccatagtgtt cccaattatt
     2821 gcctttgtct gtagacttat aaactccgtt aaatccaata aatcctacag aatcattaac
     2881 aaattgtacg ttcttaaagt atgtttcatt ggtgttaaca atggtattcc aattgacacc
     2941 actattagtt gttcttataa taattctcgt tggagaaccc cacattggtg ggggatagtt
     3001 tccaccgaca attacgcctg tatttccatc tatgaaatgt aaagctctta ataatgtatt
     3061 gccaaacctt ctgctaaccc agttatttcc tgcatcagta gtttttaaaa tcaaaccgct
     3121 gtcacctaca gcccagccag tagtttcatt caaaaatctt attgaaaata aatttaaagt
     3181 agtaccacta ttttgatgca cccaaccagt ttgagcattt acattcacat aaagatgaaa
     3241 taaaagaaat attgggatga aggatctgaa caaataatat attgagatct ctgtatattt
     3301 ggatttttca ggatttctca tttgtaaaaa agtaaacttg aagaatatta gttataatat
     3361 ttactatttt caatactttt ttaagtcatg tatctcaatc gatctctgtt aatattgttc
     3421 tttgttgatt gtactgactg gttagataaa caatttttct aaggatgatt tcttgttttc
     3481 aagtttatat aaaaaatcta ttcgaaaaat caaagtcttt aagtttaaat ctggtaaaac
     3541 cctctctccc gcacagaaaa caactttcaa ttttcaattt taaactattt tttaaatgac
     3601 aaaaaaaaaa
//
LOCUS       DAORVQ010000276         3604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_21201_length_3604_cov_4.199211, whole genome shotgun sequence.
ACCESSION   DAORVQ010000276 DAORVQ010000000
VERSION     DAORVQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21201_length_3604_cov_4.199211"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /gene="crtI"
                     /locus_tag="PLX80_13985"
     CDS             complement(<1..1437)
                     /gene="crtI"
                     /locus_tag="PLX80_13985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HQY21947.1"
                     /translation="MDKIIVCGAGIGGMVTAIRLAHMGYEVEIYEKNISAGGKMGEYF
                     ESGFRCDTCLSILSMPYVFRDFFNDIGKNIDDYFKLIPLVNSCRYFWKDNTIFNAYTD
                     SESIKEEIISVFGKNEYNGFKDYLEYAEFFFDISEKNILESEFKIKNFLNAKGIKNIN
                     KFFSGKSINDLSNKFFTDPKLQQLFDRYSTFNGSSPYLSPQIFSIIPYIEFKFGTCYV
                     DGGIYKIAKALVRLCTEYNIKINYGQRLKDIEFSSFKINKCFFEIGDNEMSEVTDFDL
                     IVSNFTNNRKLAGKEYFKSSDWSNSGFFAIMGFDGVSENLSQHNILFSDNYENEFIDI
                     FEKKLPAEDMTVYISISKKVHKEDAPEGMENWIVYVNAPVVTDKFSWNEKHKFEYFDK
                     IINKINSFNIIKDDLREKIKLFKIITPEDMMNMYGSEFGSLYGLSSNSLMTMFRRPKN
                     RSDYYENLFFTGGNTHPGGGVPLCFLSGK"
     gene            complement(1486..>3604)
                     /locus_tag="PLX80_13990"
     CDS             complement(1486..>3604)
                     /locus_tag="PLX80_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HQY21948.1"
                     /translation="YISPEEFLHILNFLRISRIIKKMISEDSRDFSEDFSVLKRITSD
                     LFFDKILEHNIDITIDETGAVKDSASSNLRKIRRQIITQSGRLRKTLANVMEKISEKD
                     YAREEIITQRDGRFVIPVKVENKRLVHGIIHSSSSTGATVFIEPTETIELNNEITELY
                     FEEKREIESILRELASQLSKNIEPIKNNIRILAEIDFLQAKAKYSIETISSKPIISEN
                     HLNLINAYHPVLLQTHKRNEVVPLDICIGDEFNTMVISGPNAGGKTVVLKTVGLNQLM
                     LQSGLHVPAAPESEFRLFDNIFVSIGDDQSLENDLSTFSSHLKYIKEIISKSDQKSLV
                     LIDEIASGTDPVLGSALSASILEYLSENNCISIVTTHNSELKEFAYSTENIENGSLEF
                     NVDNLSPNFKFSTGVPGQSFTFEIARKFDFPEVLLNNAGKYLSENESRLEDLLKELNE
                     TKQKFESLKSKYDIENTRLAGLTNIYDQKIKEFKKNEKELRYKAKLDAENIIKNANKL
                     IEKTIKEIRENENSHKEIKKHFSEAAAELTKPDIEDAKEDTEEINKVISGELKINDPV
                     QIKGTKSTGEIISISGNTVTISVNGLAIKTTIDELEKLEKPIKEKKEHSVNSFVEVNE
                     GVINRSLDIRGKYTFEITDSLYRFIDESIRIGLKEISVIHGKGSGKLRDEVHNLLKTN
                     KSVLSYRLGGLSEGDTGVTVIEI"
BASE COUNT         1111 a          625 c          528 g         1340 t
ORIGIN      
        1 ctttcctgaa aggaaacata aaggaactcc gcctcccgga tgagtattgc ctcctgtaaa
       61 aaataaattt tcataatagt ctgatctgtt cttaggtctg cgaaacatag tcataagtga
      121 atttgaagat aatccatata aagatccgaa ttctgatcca tacatattca tcatatcttc
      181 aggagttatt attttaaata atttaatttt ttctctgaga tcatctttaa tgatattaaa
      241 ggaatttatt ttatttatta tcttatcaaa gtattcaaat ttatgttttt cattccagct
      301 aaacttatca gttactactg gtgcgttcac atatacaatc cagttttcca ttccttcggg
      361 tgcatcttcc ttatgcactt ttttagatat agaaatataa acagtcatat cttcagccgg
      421 taattttttt tcaaaaatat caatgaattc attttcataa ttatctgaaa ataaaatatt
      481 atgctgagaa agattttctg aaactccatc aaaacccatt attgcaaaga atcctgaatt
      541 tgaccagtca gaagatttga aatattcttt acctgcaagt tttctgttgt tagtaaaatt
      601 agaaactatc aaatcgaaat ctgtaacttc cgacatttca ttgtcaccga tttcgaaaaa
      661 gcatttattt attttaaatg atgaaaactc aatatctttg agccgctgtc cgtaattaat
      721 tttaatatta tattcagtgc acaatcttac aagggctttt gcaattttat aaattcctcc
      781 atctacataa catgtcccaa atttgaattc tatatatggt attattgaaa atatctgagg
      841 agacaggtaa ggggatgaac cgttaaaagt agagtatctg tcaaaaagct gctgcagttt
      901 aggatcagta aaaaatttat ttgaaagatc gtttattgat tttcctgaaa agaatttatt
      961 tatatttttt attccttttg cattcaaaaa attctttatc ttaaattcac tttcaagaat
     1021 gtttttctca gaaatatcaa agaagaattc agcgtattcg aggtaatctt taaagccgtt
     1081 atattcattt tttccaaaga cagatatgat ctcttccttt atgctttctg aatctgtgta
     1141 ggcattaaat attgtattat ccttccagaa atatctgcac gaatttacga gaggaatcag
     1201 tttaaaataa tcatcaatat tttttccgat atcattaaaa aaatcccgaa agacataagg
     1261 catcgaaaga atagacagac atgtatcaca tctgaatccg gattcaaaat attctcccat
     1321 tttcccgccg gcggaaatat ttttttcata gatctctact tcatagccca tatgagccag
     1381 tctaatagcc gttaccattc cgccaatacc tgcaccgcaa acaattattt tatccatata
     1441 atcttaaata aaattaaaga taatttaaat ttgataatta acattttaaa tttcaattac
     1501 cgttacacct gtatcgcctt cagacagtcc accaagtctg tatgataata ctgacttatt
     1561 tgttttgagt aagttatgaa cttcatcacg cagcttacca ctgccttttc cgtgaattac
     1621 acttatttct ttcagaccaa ttctgattga ttcgtcaata aacctgtaca atgaatccgt
     1681 tatctcgaaa gtatattttc cacggatatc cagcgatctg tttataactc cttcattcac
     1741 ttccacaaat gaatttacag aatgctcttt tttttctttt ataggtttct caagtttttc
     1801 taattcatca atagttgtct ttattgcaag accgtttaca cttatagtta ctgtatttcc
     1861 tgaaatggaa attatttcac ctgttgattt tgtacctttg atctgtacag gatcattaat
     1921 tttcaattcg cctgaaatta ctttatttat ttcttcagta tcttctttgg catcttcaat
     1981 atcaggtttt gttagttctg cagcagcttc agaaaaatgt tttttaattt ctttatgcga
     2041 attttcattt tctctgattt cttttatagt tttctcgatc agcttatttg catttttaat
     2101 aatattttca gcatctagtt tagctttata acggagttct ttttcattct ttttaaactc
     2161 ttttatcttc tgatcataaa tgtttgtcag tccggcaagt cttgtatttt caatgtcata
     2221 cttactttta agagattcaa atttctgttt tgtttcatta agttctttca gcagatcttc
     2281 aagtctgctt tcgttttcgg ataaatattt tcccgcatta tttaaaagta cttcaggaaa
     2341 atcaaatttc ctggcaatct caaaagtaaa actctgaccc ggaacgccgg ttgagaattt
     2401 gaaatttgga gaaagattat cgacattaaa ttcaagtgaa ccgttctcta tattttctgt
     2461 gctgtatgca aactctttaa gttcgctgtt atgagtggtt actatgctga tgcaattatt
     2521 ttcagataaa tattccagga tagaagctga cagggcactt ccaagaacag ggtcagtacc
     2581 gcttgcaatt tcatcaataa gtacaagtga tttttgatca gactttgaaa taatttcttt
     2641 gatatacttt aaatgcgaac taaatgtact cagatcattt tccagggact gatcatctcc
     2701 aatacttaca aaaatattat caaacaatct gaattcagat tcaggtgctg ccggaacgtg
     2761 aagacctgac tgaagcataa gttgattcag tccgacagtt ttcaaaacta ctgttttacc
     2821 gccggcattt ggacctgaaa tgaccatagt gttaaattcg tctccaatgc agatatcgag
     2881 cggaactact tcatttcttt tatgagtctg gagtaaaacc ggatgatatg cattaatgag
     2941 atttaaatgg ttctcagata ttataggttt acttgaaata gtttcaatag agtatttagc
     3001 ttttgcctga aggaaatcaa tctcagcaag tattctgata ttgtttttta tcggttcaat
     3061 atttttagaa agttgtgaag ctagttcacg aagaatagat tcgatctctc ttttttcttc
     3121 aaaatataat tcagtaattt cattgttgag ttcaatcgtt tcagtaggtt cgataaaaac
     3181 agtagcccct gtagaagatg aactgtgaat aattccgtga acgagtcgtt tattttcaac
     3241 tttaacagga atgacaaatc tgccgtcacg ttgagtgatg atctcttcgc gtgcataatc
     3301 tttttcagat attttctcca ttacatttgc caaagtcttc cttagtctgc ctgactgtgt
     3361 aatgatctgt cttctgatct ttctgagatt ggaagatgca gaatctttta cagcgccggt
     3421 ttcatcaatg gtgatatcaa tattatgttc aagtatttta tcaaagaaga gatcggaagt
     3481 aattcttttc aatacagaaa agtcttcact gaaatctctg ctgtcttcgg aaatcatctt
     3541 tttaatgatc cgagaaattc tcagaaaatt cagaatatgt aagaattctt ccggggaaat
     3601 gtaa
//
LOCUS       DAORVQ010000277         3576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_21490_length_3576_cov_4.306447, whole genome shotgun sequence.
ACCESSION   DAORVQ010000277 DAORVQ010000000
VERSION     DAORVQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21490_length_3576_cov_4.306447"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..2673)
                     /locus_tag="PLX80_13995"
     CDS             complement(172..2673)
                     /locus_tag="PLX80_13995"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY21949.1"
                     /translation="MKIKFIIFLSAILMSLAATNSNAQQDVNGWYWINGQPVGQQINW
                     VNVFDAANMFAVTNRGMFMKSTDGGDTWTITQAGANDGSTTSGNSKRNLTTGWFFNGN
                     IGLVAGASQTSPNATVIQKTINGGATFNTIVINGVSGVTINGFYFINATTGYLCGGAN
                     ALLYKTTDQGDTWTAVPNVPSNTYYSIHAIDENNILLGSSSRRIVRTTNAGTTWITDT
                     LFTAATNVQFLGMTFKDANTGYVIGNPNYFAYTTNAGVNWTASTHSSTKGQRSIVYDN
                     GVVWTAGDYEYVYSSSNNGVTWDSVKFYDNSNPNQPLPFIIYGIGAKGNDFAVVGADG
                     QMTTSNDGGASWRNKNYTVGNLFNYSIYNTDPTGDMWVSGNSGGMMFSSNGGTNWSTQ
                     TTSHTESIYDVDFINSSTGFIAGGRANSLLGEFSNTANGGATWNYIALPSPFNSYQMN
                     SVDFVNANTGWIGGIQGPFSPAIIGKSTDGGATWVQQSIETSPNGGVTKIEMIDANVG
                     YFVGNSLYSTTNGGTNWVKNINPYVTGTSWSSMDVLNKDVIFLCGGGTGGAKKVIKTL
                     DGGLTWTDVTSNLSTAPMTAFRTEWLNLLHGTVSGTNGLNAITTNGGATWTTSNPGFS
                     TTVALSFPEKNTWFTASDRNGSYQIGRKYESNTSISVNVTMSIEGFWNGKPMVQDTVT
                     IELHNSTSPFALVDSKSAYVTRNGYQTYEFTSVASGSYYIVIKHRNGLETWSAAPIAM
                     NQGDNINYDFTTASSMAFGNNMVLKAGEYCIFSGDANQDGVIDATDLGLIDNDAFSFV
                     SGYVSTDCNGDNVIDLTDAGIADNNANNFVQVIKP"
     gene            2846..>3576
                     /locus_tag="PLX80_14000"
     CDS             2846..>3576
                     /locus_tag="PLX80_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21950.1"
                     /translation="MNIINYKLISFLNLLSKEDLSEFKKFISSPIYSSGRNYIPLLDE
                     ILLFKNDKADEISAVELYNKVYPDKKYSGQTLKNRFSELLKLGEEFIIYKDVQKDQIE
                     RDNILLKAYLDNKMFRFYEGKYKKAYSYLDSQKTNDKKFSDIYDLNQKNLHYLTLTEK
                     IDRFYSNLYEHTIYSTSISLISLFDYGIEFKQQVYLGRKYEFNMVLNILKKIDLDDIL
                     GNYEGKDLEILKVVKMYYYLYKAYD"
BASE COUNT         1160 a          639 c          554 g         1223 t
ORIGIN      
        1 aaattatttt tttggtttgg ggaaagttcg ggattttgta tcgtgtaatt taaaaaatta
       61 tattcaaaaa aaaagaccgg aagatttctc ctccggtctt tctccaacat aactaactaa
      121 ttaactaact aactaactaa ctaaagaaca ctcttgataa gttgtatgtt gttatggttt
      181 tattacttgt acaaaattgt ttgcgttatt atctgcgatt ccagcatcag tcagatcgat
      241 aacattatct ccgttacagt ctgttgaaac ataacctgaa acaaaactaa atgcatcgtt
      301 atcaattaaa ccaagatctg tagcatctat aacaccgtcc tgattagcat caccactgaa
      361 gatacaatat tcacctgctt taagaaccat gttgtttccg aatgccattg atgaagcagt
      421 tgtgaaatca tagttaatgt tatcaccttg gttcatagcg atcggtgctg cactccatgt
      481 ctcaagaccg tttctgtgct tgataacaat gtagtaagat cctgatgcaa ctgaggtaaa
      541 ttcatatgtc tgatatccgt ttcgtgttac atatgcactc tttgaatcaa caagtgcaaa
      601 tggtgatgtt gaattgtgaa gttctattgt aactgtatcc tgtaccattg gttttccatt
      661 ccagaaacct tctattgaca tagtaacatt cacagatata gaagtatttg attcatattt
      721 tcttccgatc tgatatgaac cgtttctgtc tgaagctgta aaccaagtat ttttttccgg
      781 gaatgataat gcaacagtag tagagaatcc agggttactt gtagtccatg tagctccacc
      841 gtttgttgta attgcattaa ggccgtttgt accgctgaca gttccatgta aaagatttaa
      901 ccactcggtt ctaaatgctg tcattggtgc tgttgacaaa ttagaagtta catctgtcca
      961 ggtaagaccg ccatccagtg tttttataac tttctttgct ccgcctgttc caccaccgca
     1021 aaggaatatt acgtccttat ttaaaacatc catactgctc catgatgtac cggtaacata
     1081 aggattaata tttttaaccc agtttgttcc gccatttgta gtggaataaa gtgaattacc
     1141 aacaaagtat cctacatttg catcaatcat ttcaatcttt gtaactccac cgtttggtga
     1201 tgtctctatg gactgctgaa cccaggtagc accgccgtca gttgatttac ctataattgc
     1261 gggtgaaaat ggaccttgaa ttccgcctat ccatcctgtg tttgcattta caaagtccac
     1321 tgaattcatt tgatatgaat taaatggtga aggtaatgca atataattcc atgtagcacc
     1381 gccatttgct gtattagaaa actcacctaa taaggaatta gctcttcctc cggcaataaa
     1441 accagtactg gaattaataa aatctacatc atatattgat tcagtatgtg aagtagtttg
     1501 tgttgaccag tttgttcctc cgtttgaaga gaacatcatt ccaccactat ttccacttac
     1561 ccacatgtct ccggtaggat cggtattata gattgaatag ttaaaaagat tacctacagt
     1621 ataattttta tttctccatg aagcaccgcc gtcattactt gttgtcattt gtccatctgc
     1681 accgactacg gcaaaatcat ttcctttagc accaattccg taaattataa aaggtaaagg
     1741 ctgatttgga ttactgttgt catagaactt tacagaatcc caggttactc cattatttga
     1801 agagctgtaa acatattcat aatcacctgc tgtccatact acaccattat cataaacaat
     1861 tgaccgttgt cctttagttg aagaatgagt agaagcagtc cagtttacac ctgcatttgt
     1921 tgtgtatgca aaatagtttg gatttccaat aacatatcct gtatttgcat ctttaaatgt
     1981 cattcccaga aattgaacat ttgttgctgc tgtaaaaagt gtatctgtga tccatgtcgt
     2041 tcctgcatta gttgttctta ctattcttct tgaagaagaa cctaataaaa tattattttc
     2101 atcaattgca tgaattgaat aataagtatt acttggcaca tttggtactg ctgtccaggt
     2161 atcaccctgg tcagtagttt tatataataa tgcatttgca ccaccgcata aatatcctgt
     2221 agttgcatta ataaaataaa aaccatttat agtaacacct gaaacaccat taattacaat
     2281 agtattaaaa gtagctccac cattaatagt tttttgtata actgtagcgt ttggggaagt
     2341 ttgacttgct cctgctacta accctatatt accattaaaa aaccaacctg tggtcagatt
     2401 tcttttactg ttaccgctgg ttgtgcttcc gtcattagca cctgcctgcg taatagtcca
     2461 tgtatctcca ccgtcagtag atttcataaa catacctctg tttgttactg caaacatatt
     2521 agctgcatca aatacattga cccagtttat ttgctgtccc accggctgac cattgatcca
     2581 gtaccaaccg tttacatcct gctgagcatt agaatttgtt gctgctaagc tcattagtat
     2641 tgctgataaa aatataataa attttatttt cattaccgtt ttttttagtt aattaaaaat
     2701 ttgtttatac aaaataaaaa attcattaaa ttattacaaa gaaagctatc gataataata
     2761 cccaaatatt ttacagagaa ttattaaaat gtttacctta ttgtaaattt taggtatttt
     2821 caaaaactaa ctatacccat aactattgaa cataattaac tataaactga taagttttct
     2881 taatttatta tcaaaagaag acttatcaga atttaagaaa ttcatttctt ctcccattta
     2941 ttcttcaggc agaaactaca taccattact ggatgaaatt cttctgttta aaaatgataa
     3001 agcagatgaa atttccgctg ttgagttata taataaagta tatccggaca agaaatacag
     3061 tggacagact ttaaaaaaca gattctcaga attacttaaa cttggtgaag agtttattat
     3121 atataaggat gttcaaaaag atcagattga aagagacaac atacttctaa aagcatatct
     3181 tgataataag atgtttagat tttatgaagg taaatacaag aaagcttaca gttatttaga
     3241 ttctcaaaag accaatgaca aaaaattttc tgatatatat gatttaaatc agaaaaatct
     3301 tcattatctc acattgacag aaaaaataga caggttttat tctaatctgt atgagcatac
     3361 aatttattct acttcaattt ctcttatatc actgtttgac tatggtatag aattcaagca
     3421 gcaggtatat cttgggagaa aatatgaatt caatatggta ctgaatattt taaaaaaaat
     3481 tgatctggat gatattttag gaaattatga aggtaaagat cttgaaatac ttaaagtagt
     3541 aaaaatgtat tattatctgt ataaagcata tgatga
//
LOCUS       DAORVQ010000278         3570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_21554_length_3570_cov_5.893599, whole genome shotgun sequence.
ACCESSION   DAORVQ010000278 DAORVQ010000000
VERSION     DAORVQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21554_length_3570_cov_5.893599"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1388)
                     /gene="mnmE"
                     /locus_tag="PLX80_14005"
     CDS             complement(3..1388)
                     /gene="mnmE"
                     /locus_tag="PLX80_14005"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQY21951.1"
                     /translation="MNINLTDTIAAISTAPGEGGISVIRISGKNSFEITEKIFSKNKN
                     EFSQLIISDSASHTIHFGYIFNGDILVDEVLISIFKDPNSYTGEDVIEISSHGGNYIA
                     QKILLLVINNGARHAEPGEFTKRAFLNGKMDLSQAEAVADLIGSQTEAAHTSSIRQLE
                     GSLTELVNEVKDEIINVTSLLELELDFAEEDLEFVKKDEVQHRVEKLISRLEKIISSY
                     IKGKVIREGVRLVIAGKPNSGKSSLFNYLLKTNRAIVSDIAGTTRDFIEEKLIINGVL
                     FNLTDTAGIRSSEDLIESEGIKRSYSKIEEADLILFLNDSSESEVDISNNIETFKKDF
                     DLKKSIMIFTKSDLNKLQKFKEGIHISLLKDETIEDLKKKMLHKISIENVTRQKSDEI
                     IITNLRHKICLEDVVTSLSNTLVTLNDGMTGEFLSVDLRDALSHLGEITGEVTNDDIL
                     NNIFRNFCIGK"
     gene            complement(1541..2533)
                     /locus_tag="PLX80_14010"
     CDS             complement(1541..2533)
                     /locus_tag="PLX80_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQY21952.1"
                     /translation="MSKKFLILLVLTASLLVMFLYALTLGSADISISEILNIFSSGSG
                     TSKDIILNIRLPRILNAMIVGASLSCAGLLLQSMLRNNLAEPGLLGISAGAGLGAILT
                     FIVPVTFSFFLVTPVSFIFAIFTTLIIFYFAKGFKSKSNNFISTNKIILTGIAISAFI
                     SSINGLLLLMSGSSVMQIIYWLNGGLSGRGWNEFNLGIFFAVAGIAGSVLISKDLNIL
                     NLSEELAISLGLDLKKFQRICILLSSVLAATAVSIAGIISFVGLIVPNLSKLLIGNDH
                     RYAVPCSVILGALFLLAADTISRMVIAPSEIPVGIVTSLIGAPVFIWLIFKKNK"
     gene            2594..>3570
                     /locus_tag="PLX80_14015"
     CDS             2594..>3570
                     /locus_tag="PLX80_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQY21953.1"
                     /translation="MNKFGSFNIKIALITIGVVIAIVILIYTQILAQRIQDRERDIAG
                     LYAKSMEYLANDDNSSGEYGFIFDQILPQINFPIIATDREQKEIILTKNVDYDTTMSP
                     DKKRNHLMKIARDMDEINPPIVVSYKDSVILNYVHYGQSRLVTELKLLPIFELITAII
                     FVFLGYIGFSYIKKNEQSNIWVGLSRETAHQLGTPLSSLMGWVEMLKNIEPQTPALIE
                     TTNEISSDVDKLGKIAGRFSKIGSQPKLINENLTSVIRKVSDYFEKRIPTLTSEDGQT
                     KKKVELIINSPGEINAFINKDLFEWVIENLMKNAIDAMDRSDGKIEFNIS"
BASE COUNT         1167 a          639 c          623 g         1141 t
ORIGIN      
        1 cttcactttc cgatacagaa atttctaaaa atgttattta aaatatcatc attggtcacc
       61 tcacctgtga tctccccgag atgcgagagt gcatcacgca gatcgacgga taaaaattcg
      121 ccggtcattc cgtcattcaa agtgacaagt gtgttgctca aggaagtgac tacatcttcg
      181 agacagattt tgtgacgaag atttgtgatt attatttcat ctgacttttg gcgggttaca
      241 ttctctattg aaattttatg cagcattttc ttttttaaat cttcaatagt ttcatctttg
      301 agaagtgaaa tgtgaatccc ctctttaaat ttttgaagtt tgttcagatc cgatttggta
      361 aatatcataa tcgatttttt taaatcgaag tcttttttaa atgtctctat attattcgaa
      421 atatctactt cactttcaga tgaatcattc agaaataata tcagatctgc ctcctctatc
      481 ttactgtaac tccttttgat cccttcgctt tctatcaggt cttctgaaga tctgatccct
      541 gccgtgtcag ttagattgaa aagcacacca ttaatgatca gcttttcttc aataaaatcc
      601 ctagtagtac cggcaatgtc actgacaatt gcacggttag ttttcagaag ataattaaaa
      661 agtgaagact taccgctgtt tggcttacct gctatcacaa gtcttacacc ttcgcggatt
      721 actttgcctt ttatataaga agaaataatt ttttctaaac gggaaattaa cttttcaact
      781 ctatgctgca cttcatcttt ttttacaaac tcaagatctt cttcagcgaa gtctagttcg
      841 agttcgagaa gtgatgttac atttataatt tcatccttca cttcattcac gagttcggtc
      901 aacgaacctt cgagctgcct gatcgaagat gtatgcgctg cttcagtctg agagccgatc
      961 agatcagcaa ctgcttcagc ctgcgagaga tccattttgc cgttaagaaa tgcacgcttt
     1021 gtaaattcac ccggctcggc atgtctggca ccgttattaa taaccagtaa taaaattttt
     1081 tgtgcaatgt aatttcctcc atgagatgaa atttcaatta catcttcacc agtataagaa
     1141 ttcggatctt taaaaattga gatcagaact tcgtctacca aaatatctcc attaaaaatg
     1201 taaccaaagt gaatagtgtg cgaagctgag tcagatatta ttaattgaga aaattcattt
     1261 ttatttttgc tgaatatttt ttcagtgatc tcaaaagaat ttttaccaga aattcttatg
     1321 acagaaattc cgccttcgcc cggagcggtt gaaattgctg cgatagtgtc agtcagattt
     1381 atgttcatat tttattaata attatttctt ttaacaaatg cattcttccg tagagtccga
     1441 agagtacgtg actctgcggg aacggactct gtggtaactt gaattacttt ctcctgttgt
     1501 tttcaaataa acttcatatt tgaccatttt tttaatttca ttatttattt tttttaaaga
     1561 ttagccagat aaaaacaggc gcaccgatca gagaagttac aatgcccaca ggaatttccg
     1621 aaggtgcgat taccattctt gagattgtat cggctgctaa taaaaataat gcaccgagta
     1681 ttaccgaaca agggactgca tatctgtgat catttccgat aaggagtttt gataaattag
     1741 gaactatcag tccaacgaaa ctgattatac ctgcaataga aacggcagtt gcagcaagta
     1801 cagatgatag aagaatgcag atcctttgaa attttttcag gtcgagcccg agtgatattg
     1861 caagctcttc actgagattt agaatgttca gatcttttga aatgagtaca gaacctgcaa
     1921 tacctgcaac tgcaaaaaat atcccgagat taaattcatt ccatcctctt cccgacagac
     1981 ctccattcag ccagtaaatg atctgcatta ctgagctacc tgacattaga agcagtagtc
     2041 cgttaatcga tgatataaat gcgctgattg caatacctgt taatattatt ttgttggttg
     2101 aaataaaatt attacttttg ctcttaaatc cttttgcaaa ataaaaaatt atcaaagttg
     2161 tgaatattgc aaaaataaat gaaaccggtg taacgagaaa aaatgaaaat gtaacgggta
     2221 caataaaagt cagtattgct ccgagtccgg cacctgcgga gattccaaga agacctggct
     2281 ctgcaagatt atttctaagc attgactgaa gtaacagccc tgcacaagat agagaagcac
     2341 caactatcat agcattcaga attctgggaa gtcttatatt cagaattata tcttttgaag
     2401 taccgctgcc tgaggaaaaa atatttaata tttcagatat tgaaatatct gcgctgccga
     2461 gtgtgagtgc atacaaaaac attaccagca aagacgctgt taatactaaa agtattaaaa
     2521 attttttaga caataattat taaatttaaa taagtgatta tatacaatat attaaaacga
     2581 atctcatagc ttaatgaata aatttggctc atttaatata aagatcgcgc ttattactat
     2641 cggcgtggtc attgcaattg taattctcat ctacacacag atactcgcac agcggataca
     2701 ggacagagag agggacattg ccggactgta tgcaaaatca atggagtatc ttgcaaacga
     2761 tgataattct tcaggtgaat acggttttat ttttgatcag atcctaccgc agattaattt
     2821 tcctattatt gcgacagacc gtgaacagaa agagatcatc cttacaaaga atgttgatta
     2881 cgacactacc atgtctcctg ataaaaaaag aaatcatctg atgaaaatcg cacgtgatat
     2941 ggatgagatc aatccgccga tagtagtttc ttataaagat tcagtaattt taaactacgt
     3001 tcattatggt cagtcaagat tagttacaga gttaaaactt cttccgatat ttgaattaat
     3061 tacagctatt atatttgtat ttctcggata cattggattt tcttacatta aaaaaaatga
     3121 acagagtaat atctgggtag gactttccag agagactgct catcaactcg gcacaccact
     3181 ttcgtctctg atgggatggg tagaaatgct taagaatatt gaacctcaga ctcctgctct
     3241 tattgaaact acgaatgaga tcagcagtga tgtagataaa cttggtaaaa tcgcaggcag
     3301 attttctaaa attggttcac agccaaaact tataaatgaa aatctgacaa gtgttataag
     3361 aaaagtttca gattattttg agaaaaggat cccgactctt acaagtgaag atggtcagac
     3421 aaaaaagaaa gttgaattaa taataaacag tcccggagaa ataaatgctt ttataaataa
     3481 agatcttttc gaatgggtaa ttgaaaatct gatgaagaat gcaatcgatg caatggacag
     3541 aagtgacggg aaaattgaat ttaatatttc
//
LOCUS       DAORVQ010000279         3558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_21701_length_3558_cov_5.588924, whole genome shotgun sequence.
ACCESSION   DAORVQ010000279 DAORVQ010000000
VERSION     DAORVQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21701_length_3558_cov_5.588924"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLX80_14020"
     CDS             <1..229
                     /locus_tag="PLX80_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21954.1"
                     /translation="YEDVVSKNPSFIIFPADTLDTEKIEIYKKELTEKLSNSEAVKNG
                     KVIFIDDDIMFRPGPRITDAVKTLRAHIKTD"
     gene            357..2030
                     /gene="ettA"
                     /locus_tag="PLX80_14025"
     CDS             357..2030
                     /gene="ettA"
                     /locus_tag="PLX80_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQY21955.1"
                     /translation="MSDNKIIFSMVGVSKTFPPQKQVLKNIYLSFFYGAKIGIIGLNG
                     SGKSTLLKIIAGIEEKFEGEVHFTKEYSIGYLSQDPELDETKTVKEIVQEGVQEVVDV
                     LKEYEEVNNKFAEPMEDDEMTKLIEKQGELTEKIEHMNGWELDAKLERAMDALRTPEG
                     DTPVKNLSGGERRRVALCRLLLKEPDILLLDEPTNHLDAESIDWLEQHLKQYKGTVIA
                     ITHDRYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKTKRLAMEEKQESKRQKTLLHE
                     LEWVRMSAKARHAKSKARLQAYDKMLNEDTKKKEEQLEIFIPNGPRLGNKVIESLDVS
                     KSFGEKLLYEKLNFSLPPAGIVGIIGANGTGKTTLFRMILGTETPDNGKFDVGETVNI
                     GYVDQAHANIDPEKTVWEVISEGNEFIELGGKQYNSRAYVARFNFAGADQGKKVSSLS
                     GGERNRLHLALTLKSEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVIISHDRWFL
                     DRVATHILAFEGDSVVDYFEGGYSEYEEYKKKKYGYVEPKRVRYKKLVE"
     gene            complement(2132..3172)
                     /locus_tag="PLX80_14030"
     CDS             complement(2132..3172)
                     /locus_tag="PLX80_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQY21956.1"
                     /translation="MKKFTISLILLFSMFSFTKISKAQGIDTLKGASPFTSGHYVPGI
                     WDIRDFATPSPGFYVLDYNLFISGKKFFNSEGNQVTEIVRQYSKTTLDIDINGYVNIP
                     TFFYASSFKLLGGTYIAAITPPYSTVNLDLAYDAVAKVLRDTITKDGTINGNVSGFSD
                     MTFLPFGLSWASENMNLTMAYSIVAPTGRYTPSGSDNVGLGYWTNMFQAFGYVYPMKI
                     KGKSSQAMAIMASLTYEMNSKIKDVNVSPGNRLTVEYGISQYLSPRFEIGIMGGYNFQ
                     ITDDTGTDVWWDSSVHDQLGYVSFQIGAWPVTNKLYTAIKYNVDYGMKQRIKQHNFLL
                     NIIYATGWLTGK"
     gene            complement(3224..>3558)
                     /locus_tag="PLX80_14035"
     CDS             complement(3224..>3558)
                     /locus_tag="PLX80_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21957.1"
                     /translation="LKNDILNEVKTNEIQFSEKESRIKLLEDSIAQNRYDHEQLLKEV
                     NSLYPEINSISIANHTFYTENDKAEFNIPVFIYDVEKDLIPADKNKLQGWLKSRLKLD
                     SLEMYKKD"
BASE COUNT         1194 a          616 c          668 g         1080 t
ORIGIN      
        1 ttatgaagat gtggtcagca aaaatccatc tttcattatt ttccctgctg atacccttga
       61 tacagaaaag attgaaattt ataaaaaaga attaactgaa aagctgagta attctgaggc
      121 agtcaaaaac ggtaaagtaa tttttataga tgatgacatt atgttcagac ccggtccacg
      181 gatcacagac gctgtgaaga ctttgagagc tcatattaaa acggattaag ataaattttc
      241 tctcatgagc tgcaaaacat attattaatt tttagaaaat actgcaattt aaacttaaac
      301 ctctttcctc aaatcgctat attgtcactt accaaaaatt aaattaaaat aataaattgt
      361 cagataacaa aattatattc tcaatggtgg gagtaagcaa gactttccca ccgcagaagc
      421 aggtgttaaa aaatatttac ctttcatttt tttatggagc gaagatcggc atcatcggtc
      481 taaacggttc gggaaaatct acgctgctaa agatcattgc agggattgaa gaaaaattcg
      541 aaggtgaagt tcatttcaca aaagaatatt caatcggata tctttcacag gatcccgagc
      601 ttgatgaaac taaaacagta aaagaaatag tacaggaagg cgttcaggaa gttgttgatg
      661 tattaaaaga gtatgaagaa gtaaataata aattcgccga gccgatggaa gatgatgaaa
      721 tgactaagct tatcgaaaag cagggtgaac ttactgaaaa gatcgaacac atgaacggct
      781 gggaactcga cgcaaaactc gaaagagcaa tggatgcact aagaactccc gaaggagata
      841 ctcctgtgaa aaatctttca ggtggtgaga gaagaagagt tgcactatgc agactgttgc
      901 ttaaagaacc ggatattctt ttactcgatg agccgacaaa ccatctcgat gcagaatcta
      961 tcgactggct tgaacagcat cttaaacaat ataaaggtac tgtcattgcg attactcacg
     1021 acagatattt cctagacaat gttgcaggct ggatactcga gctggacagg ggagagggga
     1081 ttccgtggga aggtaattat tcctcatggc ttgatcagaa aaccaaacgt cttgcaatgg
     1141 aagagaaaca ggaaagtaaa agacagaaga ctttgctgca tgaactcgaa tgggtgagaa
     1201 tgtctgcaaa ggcaagacat gcaaaatcta aagcgagatt gcaggcttat gataaaatgc
     1261 tcaatgaaga tacaaagaaa aaagaggaac agcttgagat atttatcccg aatggtccga
     1321 ggcttggaaa taaagtgatc gaatctctgg atgtcagtaa gtcttttggt gaaaaacttt
     1381 tatatgagaa actgaatttt tcacttccac ctgcgggaat tgtcggtatc attggagcta
     1441 acggtacagg taagacgaca ttgttcagaa tgatactcgg cacagaaacg cccgacaacg
     1501 gtaaatttga tgtcggtgaa actgtaaata tcggttatgt agatcaggca catgcgaata
     1561 tcgatccgga aaaaactgta tgggaagtga tctcagaagg aaatgaattt attgaactcg
     1621 gcggaaagca gtataactca agggcgtacg tcgcaagatt taattttgcc ggtgcagatc
     1681 agggaaagaa agtaagctca ctttcaggtg gagaaaggaa tagactgcat cttgcactta
     1741 ctttaaaatc agaagctaat gtattgctgc ttgacgaacc tacaaacgac attgatgtga
     1801 atacacttcg ggcactggaa gaaggtcttg aaaattttgc aggatgtgct gttataattt
     1861 ctcacgacag atggttcctt gacagagtgg ctacacatat acttgcgttt gaaggagatt
     1921 ctgttgttga ttatttcgaa ggcggctatt cagaatatga agaatataaa aagaagaagt
     1981 atggatatgt cgagccgaag agagttagat ataaaaaatt agttgaataa aatttaatat
     2041 tcatttagta attaaaaaat tggatgactg aatctttctg tcatccaatt tttatattaa
     2101 gtttttataa attactatta aataaatctc ttcatttacc tgtcagccat cctgttgcat
     2161 aaataatatt taataaaaag ttatgctgtt taattctctg tttcattcca taatctacat
     2221 tatacttaat ggcagtataa agtttatttg tcacaggcca tgctccgatc tgaaaactta
     2281 catatccgag ctggtcgtgt acagatgagt cccaccagac atcagttccc gtatcatctg
     2341 tgatctgaaa attatatccg cccataattc cgatctcaaa tctcggactc agatactggc
     2401 ttattccgta ttcgacagtc agtctgttac ccggtgatac atttacatct ttaattttac
     2461 tgttcatctc atatgttagt gaagccataa ttgccattgc ctgacttgac tttcccttga
     2521 tcttcatcgg atacacatat ccaaatgcct gaaacatgtt tgtccagtat ccgagtccta
     2581 cattgtcact gccgctagga gtatatcttc cggtaggggc aactatagaa tatgccattg
     2641 ttaaattcat attttccgaa gcccagctta aaccaaatgg aagaaatgtc atatcactga
     2701 atccgctgac atttccatta atagttccat ctttggttat cgtatccctt aatacttttg
     2761 caactgcatc ataagcaaga tcaagattta ctgtagaata aggaggagta attgcagcga
     2821 tataggtccc gccaagtaat ttaaaggatg atgcataaaa aaaagtcgga atgtttacat
     2881 atccgttaat atctatatct aatgtcgtct tactgtactg tcgaactatt tcagtaacct
     2941 gattaccttc actgttgaaa aatttcttcc cgctgataaa caaattataa tccaacacat
     3001 aaaatcccgg agaaggagtt gcaaagtctc ttatatccca gattcccgga acgtaatgac
     3061 ctgatgtaaa tggtgaggca cccttcaatg tgtcgatacc ttgcgcttta ctaattttag
     3121 taaaactaaa catcgaaaac aaaagaatca aagagattgt aaattttttc atttttaatc
     3181 atttattttt ataaatatat ctcgcattat tcagtgaaga attttaatct tttttataca
     3241 tctcaagact atccagttta agtctgctct taagccaacc ctgaagttta tttttatcag
     3301 caggtattag atctttttca acatcataaa taaaaaccgg gatattaaat tctgctttat
     3361 cattttctgt ataaaatgta tgattggcaa ttgatattga atttatttca gggtaaagag
     3421 aattaacttc ttttaataac tgctcatggt catatctatt ctgcgcaatc gaatcttcaa
     3481 gtaattttat tcttgactct ttttcactga attgtatttc atttgttttc acttcattca
     3541 aaatatcatt tttcagtt
//
LOCUS       DAORVQ010000280         3495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_22412_length_3495_cov_4.016279, whole genome shotgun sequence.
ACCESSION   DAORVQ010000280 DAORVQ010000000
VERSION     DAORVQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22412_length_3495_cov_4.016279"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..391
                     /locus_tag="PLX80_14040"
     CDS             167..391
                     /locus_tag="PLX80_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21958.1"
                     /translation="MKNFIKIFLAVVVMVTMFSFSSVQNSEAKTSEVAVLNSETQDFG
                     LMFVKIVENGVDYIYIYTEGGIYITKVEEL"
     gene            complement(1307..2383)
                     /locus_tag="PLX80_14045"
     CDS             complement(1307..2383)
                     /locus_tag="PLX80_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HQY21959.1"
                     /translation="MKTIILNVFILCIFFVSQNSFADEVEFASGSYQEIIDKAKSENK
                     TIMIDFVTDWCKWCVETDRKVYTDPAVYSYANERQINWKIDAEKGEGPELAKKYGVKG
                     FPTIIFTDPSGTEIDRIYGYLPAEQFLIRMMDYNNGINTYSFLSANLEKEPDSPQNNF
                     LMAEKIISNGLDGDPKVYLNKAILNDPGNTLGYTDDAKFTLAYLNQSPEELQKLLTEY
                     PESEKVKDGYINLASFYEEKGEPIKTDELYTEAFSKYGISDFDLMQSYGGLLLGRAYG
                     TMKDEKATDKDKENAINTLNNCLKYVSGTVNEASAYFIMSELYFQLNQLEKAEESIDK
                     AILVYDKKSYRDQKDKISKQRAEK"
     gene            2568..3458
                     /locus_tag="PLX80_14050"
     CDS             2568..3458
                     /locus_tag="PLX80_14050"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY21960.1"
                     /translation="MKIIITGGSGLLGQYLNIYLNREHSILTVYNNSVGNCTEYNSLK
                     TDLNNTYYLSEVIKEFRPDILIHTAAVSRPEECDKLSYEYVIKINYNLTKLLSEYCNK
                     IKCRLIFTSTDLVYDGDTNKIQDESSIPKPVSLYAQTKLDSENFIRENCNDYLILRTS
                     LLYGIGLNGSSCNFHNMLISFKKGEKVRLFYDQYRTPLSLHNSAKIIDEIAKSEIKNV
                     TMNFGGSERISRSELGEMVCRKFNFDLDLIEKTSLKESVTKNKVIDVSMNTDMLRSFG
                     FSQMSIEESLEKIQSEIDAI"
BASE COUNT         1252 a          473 c          474 g         1296 t
ORIGIN      
        1 aagcttgttg tcaaattagc taattttaat aaaaaattca agcattaaca gctataagtg
       61 ttaattagtt aagaaaaaac agcgacaatt cgaattaatt ttctttgtat cgattgaata
      121 ttatccttaa tttgtagtaa ataaaattat gaaaggggat ttctaaatga aaaacttcat
      181 taagatcttt ttagcagtag tagtaatggt aacaatgttc tcgttctctt cagtacaaaa
      241 cagtgaagca aaaacatcag aagtagcagt attaaattct gaaactcagg actttggttt
      301 gatgtttgtt aaaattgttg aaaatggtgt agattacatt tatatctata ctgaaggtgg
      361 aatttacatt actaaagtag aagagttata attacaatat tacttgtaat ttaaaataag
      421 agtttaatgc tgtacaaatg cagcatataa aagtaaaacg aagttatttt ttagagtccg
      481 ctttcattgt tggatttaat aagatataag gaaagatttt gtaaaaatta aataagttac
      541 atcctcccct ctcctttcac cgttcaaaat ttggggttga acggccttgg aggatgtaac
      601 ttatctaatt tattacttga aacatgaact ttttgggtta gtctggataa aattaagaat
      661 tataaactta attgtaaagt atatttttta gatattttag ctataaaatc agatttcgtt
      721 ctttcaaaaa ccatttcata attttatttc attctgattt atatctaaaa attcttcatt
      781 ctataccttt taattagttt cattctttat ttttaaaaat taatctcaga agtcatcacg
      841 tttgaattaa aggatcaaaa taaatttatt gataagctaa ttttatggat atttaaaaat
      901 agttgtagtt tttagaattt ctattttttt aaattcataa tggttttcat taattaagtc
      961 tcatgtaaat tataaagagg gaataggtat ctttcaaatt cgatatttta atatgataga
     1021 aatcttacat cctgatttaa tttattaatt taccctcccc ctaaaaaaaa atgtaataac
     1081 ttgaatgttt tattttaata aatttataaa tctttgtatt aatgaattta ttaattttaa
     1141 taaagatgcc tgaatcagaa aattctattc atgtttatta gattctatga catttcgata
     1201 ttcttaaaaa tttatatttt atttagagtt aataaaaaat ccgataaata aatttaccgg
     1261 attagattat atattataat attttataaa gcttcaaaaa gtaatattat ttttcagctc
     1321 tttgtttaga aattttatcc ttctgatctc tgtaagactt tttatcataa actaaaatag
     1381 ccttgtcaat agattcttca gctttttcta gctggttcaa ctgaaaatac agctcagaca
     1441 taataaaata agcacttgct tcattgacag ttccactaac atatttcaga caattattta
     1501 aagtgtttat tgcattctct ttatctttat cagtagcttt ttcatctttc attgtaccat
     1561 aagctctccc aagtaacaga cctccataac tttgcatcag atcaaaatcc gaaattccgt
     1621 atttggaaaa agcttcagta tatagttcat cagtttttat gggttctcct ttttcttcat
     1681 aaaaggatgc aagattaata tatccatctt ttaccttttc actttcagga tattcagtta
     1741 aaagtttttg taattcttca ggactttggt ttaaatatgc caaagtaaat ttagcatcat
     1801 ctgtatatcc taaagtgttt ccgggatcat ttaaaattgc tttgtttaga taaacttttg
     1861 ggtcaccatc caatccgttt gagatgattt tttctgccat cagaaaatta ttctgaggac
     1921 tgtcaggttc tttttcaaga tttgcagata gaaagctgta tgtattaatc ccattattgt
     1981 aatccatcat tctaatcaaa aactgttctg caggcaaata tccgtagatc ctgtctattt
     2041 cagtacctga tggatctgta aatataattg taggaaatcc tttgactcca tatttttttg
     2101 caagttcagg tccttcccct ttttcagcat caattttcca attgatctgt ctttcatttg
     2161 cataagaata aactgcaggg tcagtgtaga cttttctgtc tgtctctaca caccacttac
     2221 accagtctgt tacaaagtca atcattattg ttttattctc gcttttggct ttatcaatta
     2281 tctcctgata tgaacctgaa gcaaattcta cttcatcagc aaatgaattc tgagatacaa
     2341 aaaatatgca tagaataaaa acatttaaga taatagtttt cattttttac tccttatatt
     2401 aatattaatt tattaaaata atttatgaaa taaggtattc atcttcaatc caatatttaa
     2461 attgattaat ctaaaaaatt agatccaaat ttactgatat agtttagatt ttaagattta
     2521 taataagata catttaattt tgaaaaaagt tttaatttaa aaaaaatatg aaaataatca
     2581 tcacaggcgg aagcggtttg ttaggtcagt accttaacat atatttgaac agagaacatt
     2641 ctatacttac tgtttacaat aattctgtag gaaattgcac tgagtataat agtctaaaga
     2701 cagatctgaa taatacttat tatttgagtg aagtaataaa ggaattccga cccgatattt
     2761 tgatacacac tgctgctgtt tcaagaccag aagaatgtga caaactttct tatgaatatg
     2821 ttattaaaat aaattataat cttacaaagt tattatcaga atattgtaat aaaataaaat
     2881 gcagattaat atttacttca actgaccttg tttatgatgg agatacaaat aaaattcaag
     2941 atgaaagttc aattccaaag cctgtttcat tatatgctca gacaaaactt gacagcgaaa
     3001 attttatcag agaaaattgc aatgattatt tgattcttag gacatcatta ttatacggga
     3061 taggattaaa cggctcgagc tgtaactttc ataatatgtt aatcagcttt aaaaaagggg
     3121 agaaagtcag attattttat gaccaataca ggactccatt atctctgcat aactctgcca
     3181 aaataattga tgaaattgca aaatcagaaa tcaaaaatgt aacaatgaat ttcggaggaa
     3241 gtgagagaat ttccagatca gagctcggag aaatggtctg cagaaaattt aattttgatc
     3301 tggatctaat tgaaaaaacg tcattaaaag aatcagttac taaaaataaa gttatcgacg
     3361 tttcaatgaa tacagatatg ttaaggtctt tcggattcag tcaaatgagt attgaagaat
     3421 ctttagaaaa aatacaatct gaaattgatg caatttgaaa ttataaatta atttaaagaa
     3481 ggatcttctg gaaaa
//
LOCUS       DAORVQ010000281         3493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_22427_length_3493_cov_5.261780, whole genome shotgun sequence.
ACCESSION   DAORVQ010000281 DAORVQ010000000
VERSION     DAORVQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22427_length_3493_cov_5.261780"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..2066)
                     /locus_tag="PLX80_14055"
     CDS             complement(237..2066)
                     /locus_tag="PLX80_14055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY21961.1"
                     /translation="MKLKTLILWGYVLMGAIIIALSSFSIYFIERLNSASEKILKDNY
                     LSIESANKLIDNLDIIENSQAILLSKKQIEKDISSREYILAKKAFSEYLIKCEGNITE
                     TGESEKLKDIRKQFDIYISKIENADTAFSDIKYFNELIPEYKKVKKLCYDLLELNEKA
                     ILNKSENSNKISKETELLMLIVAGMSILFVALIIIKAPSTIIQPILELTKKVEGISNK
                     KYSERIEVKSDNEVGALAVSFNKMAEKLSEYEKSNIEKIIVEKKRAEAIVENMRDAII
                     VLDEKDIVIFANKVSSELLGIEQTELVGRHVDKIAAANNLFKNIIDSRKQNESYKSED
                     KNKYLRIVNNNKEEFYQKDFTKISYSNTEIIGTIIVLKNVTGFKELDEIKSGFVATVS
                     HELKTPLAAMNMSLRLLQDIRVGELNNEQKKLTSSMKEEVKRLLKMVNELLNLAKLES
                     GGEIYKYQEISLEELVDAAVTPMLMQLEQKNINFDLEIEKGLPKLILDVNKIAWVIIN
                     LLNNAVRYTYEGGEIKFKVSRENNFVKFSVSDNGVGIKPEYIDKIFQKFVQVNKENTG
                     NQYKGVGLGLAISKEFIEAHKGVIYVTSEYEKGSEFVFKLPVL"
     gene            complement(2070..3203)
                     /locus_tag="PLX80_14060"
     CDS             complement(2070..3203)
                     /locus_tag="PLX80_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
                     /protein_id="HQY21962.1"
                     /translation="MENKIINSDAEKFLELIKRSRNGKFKIYIGLAAGVGKSYRMLLE
                     AHELLKNNIDIQVGYIETHGREETQKLLEGLPFIPRKIIYYKGKELEEFDIDTVLKRR
                     PELVIVDELAHTNAPGCRHEKRYQDVEELLYNGINVISALNIQHIESLNRVVQEISGI
                     EVKETIPDRIIALADEVVNIDLTADELISRLKSGKIYKKDKIDSALSNFFKKDNLLQL
                     RELALREVADQVEKKIIFEVPRSERYDKEKILVCIGNDYNQNKKIIRHSYRIADRLDS
                     KWFVLYVETNSEPFEDLDLAIQRHLINNFKMATDLGASSEKIKAGSIVEGIIIYSKEK
                     EITKIVMGRPDDKKSFKKILKGNIVNKLMNAMKDIDCDIEIIT"
BASE COUNT          987 a          624 c          462 g         1420 t
ORIGIN      
        1 ctttgtggct ttgtggcttt gtggctttgt ggctttgtgg ctttgtggct ttgtgtcttt
       61 gtggctttgt ggctttgtag ctttgtgtct ttgtggcttt gtagctttgt ggctttgtgt
      121 ctttgtgtct ttgtgtcttt gtggtattaa ttaaaattat gttttgagac actttctttc
      181 cagggagggg gaggcttaaa ttgcaattag atcattacaa tttgcaaaga ccttttttat
      241 aatacaggca gtttaaacac aaactcactt cctttctcat attcacttgt cacgtaaatt
      301 actcctttat gagcttcaat gaattcttta ctaattgcca gcccaagtcc aacacctttg
      361 tactgatttc cggtgttttc tttgttaacc tgtacaaact tctgaaatat tttgtctata
      421 tattccggct taataccaac accgttatca cttacagaga attttacaaa attattttcc
      481 ctgctgactt taaatttaat ttcaccacct tcataagtat atcttactgc attatttaac
      541 aaattaataa tcacccatgc aattttattt acatcaagta ttaatttcgg taaacctttt
      601 tcgatctcca aatcgaagtt tatatttttc tgctccagct gcatcagcat tggtgttact
      661 gcagcatcaa ccagctcttc caaagaaatt tcctgatatt tatatatctc accgcctgat
      721 tcaagtttgg caagattaag caattcattt accattttca ataaacgttt tacttcttct
      781 ttcattgaag atgttaattt tttctgttcg ttgtttaatt ctccgactct gatatcctga
      841 agaagtctta aactcatatt catagcagcc agtggtgttt tcagctcatg agaaactgta
      901 gctacaaacc cactttttat ttcatccagc tctttaaagc ctgttacatt tttcaaaact
      961 attattgtac caataatctc tgtatttgaa taggatattt tagtaaaatc tttttgataa
     1021 aactcctctt tattattatt gacgattcgg agatatttat ttttatcttc agatttgtaa
     1081 gattcatttt gtttcctgct gtcaataata tttttaaaaa ggttatttgc agctgctatt
     1141 ttgtctacat gtcttcctac taattcagtc tgttcaatac cgagaagttc tgagcttact
     1201 ttatttgcaa aaattactat atccttttca tccagtacta tgattgcatc tctcatgttt
     1261 tcgacgatgg cttcagcgcg ttttttctct actataattt tttcaatatt ggatttttca
     1321 tattcactta atttttctgc cattttgtta aatgaaacag ccagtgctcc gacttcatta
     1381 tcggatttta cctctattct ttcggaatat tttttattcg aaatgccttc aacttttttt
     1441 gttagttcaa gtatgggctg aatgattgta gatggagctt taataataat taatgcaaca
     1501 aacaatattg acatacctgc aactataagc attaataatt ctgtctcttt ggagatcttg
     1561 tttgaatttt cacttttatt aagtattgct ttttcattca gctccagcag atcatagcaa
     1621 agttttttta cttttttgta ttcaggaatt aattcattaa aatattttat atcggaaaaa
     1681 gctgtatctg cgttctctat ttttgatata taaatatcaa attgctttct gatatctttt
     1741 aatttttctg attcacctgt ttcagtaatg tttccttcac actttattaa atattctgaa
     1801 aaggcttttt ttgcaagtat gtactccctg cttgaaatat ctttttcaat ttgttttttt
     1861 gaaagaagaa tggcttgtga attttcaata atatccagat tatctatcaa tttatttgct
     1921 gattcaattg aaagatagtt gtcttttaaa attttttcag aagctgagtt taaacgttca
     1981 ataaaataaa tactgaaaga actgagcgca ataattattg cccccattag aacatatccc
     2041 cagagtatta atgtttttaa tttcattggt caggttataa tttcaatatc acaatcaata
     2101 tctttcatag cattcattag tttatttact atattccctt tcaatatttt tttaaaagac
     2161 tttttatcat caggtctgcc cataactatt ttggtgatct ctttctcctt tgaataaata
     2221 attattcctt caactataga gcctgccttt attttttcag aagaagctcc gagatcggta
     2281 gccatcttga aattattgat cagatgtctt tgaattgcaa gatcaagatc ctcaaacggt
     2341 tcactgttgg tttccacata caatacaaac cattttgaat ccaatcggtc agcaattctg
     2401 tagctgtgcc tgattatttt tttattctga ttgtaatcat ttccaatgca tacaagaatc
     2461 ttttccttgt catatctttc tgatctggga acttcaaata taattttttt ttcaacctga
     2521 tcagcaactt ccctcaaagc aagttcacga agctgaagca gattatcctt tttaaaaaaa
     2581 ttagataatg cagaatcaat tttatccttt ttatagatct ttcctgattt taaacgactg
     2641 ataagttcgt ctgcagtaag atcaatattt accacttcat cagccagggc tatgatacga
     2701 tccggaatag tttcctttac ttcaattccc gatatttcct gaactaccct gttgagactt
     2761 tctatatgct gaatgtttaa tgcgcttatt acattaattc cattatataa aagttcttcg
     2821 acatcctgat atcttttttc atgtctgcat cccggagcat ttgtatgtgc aagttcatca
     2881 acgatcacaa gctcaggtct ccttttcaag acagtatcaa tatcaaattc ttcaagctct
     2941 ttgcctttat aataaattat ttttctggga atgaacggaa gtccttccaa aagcttttgg
     3001 gtttcttccc ttccatgtgt ttcaatataa ccgacctgaa tatcaatatt gttttttaac
     3061 agttcatgtg cttcaagcag catcctgtat gacttaccta cacctgcagc aagtccgatg
     3121 tatatcttga attttccatt ccttgatctt ttaataagtt caagaaattt ctcagcgtca
     3181 gaattaataa ttttattttc cataaattat atattcatga tttaaagtga ctgataccac
     3241 acaggtatat taatttttga ttgatttaac ttaatagaat gattactaaa ataatataaa
     3301 aatattatta atattctaaa taaaatttca gaactattta agtttatcca gttcaatatt
     3361 aagtttcaga acatttaatt tttctatacc aataatacct aaatactttc cttctatatt
     3421 attaatgacc agttctttta atttatccgg atcaatattc cttacttttg aaattcttgg
     3481 tatctgaata tat
//
LOCUS       DAORVQ010000282         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_22480_length_3488_cov_6.258666, whole genome shotgun sequence.
ACCESSION   DAORVQ010000282 DAORVQ010000000
VERSION     DAORVQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22480_length_3488_cov_6.258666"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLX80_14065"
     CDS             <1..1227
                     /locus_tag="PLX80_14065"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21963.1"
                     /translation="NNRPIVDITFTDSLTGYAIASNSGTADSSYILKTTNGGDNWYIL
                     SIEYKSYYAISFIDNETGYVGGSDLMGYSYLIKTTNGGINWIRQNSPSILGLTDLAIQ
                     NEDTIWITDPNGLDGGIFRTTNGGTNWTIQQQPGSSIEKIYMYNKNIGFASSTSRLYK
                     TTNSGLNWNLIPGQNAYRDIYFIDSLNGWKSYDSMKITTDGGFTWKPQLIKSPGIPLF
                     STIATFSKISNDTIWATGLVYEFPNLQVRSVIHRTTNAGRDWYFQIPDTSIRVGVFRT
                     NFINTITGWNYGNLSGLYTNTGGDSIFNPITSINEFSIIENPSFYKLFQNYPNPFNPT
                     TKISFELQKAGNVKLTVYDIQGKYIEVLVNEKKQTGNYEVEFDGSNLSSGVYFYRLEI
                     SGKENYSDTKRMILLK"
     gene            1311..>3488
                     /locus_tag="PLX80_14070"
     CDS             1311..>3488
                     /locus_tag="PLX80_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21964.1"
                     /translation="MKSKIVFSTGLFVVSVFMFLSLTALDNPQDKFLIVAMHSGVDTS
                     PVNYTRLKDSLGLNGWHRYNQWFTGDEINGNISFISPRVQYRVDQNNTRDLRTVFGRR
                     ITDYSAWGQRSDYQCENVTEGQPYWFYSYYNSVSNSWIHDIPDSGQTVKYCKVDTSNP
                     GRDSGYIVQGLKANREQANRFWTPYQSDTFSDWYVKPKIRIPTGLSINTKVCRIEILD
                     WDSSIVKSIELTVNEFKDGNQNYNGNYMDEFYFQPNVLSPIKIDSPGLCPGPDSLRKY
                     FWDWGSTTPLNTIKTDFRVFWYGQCDVWIDRIRVENEQARLLFENTDTKARIVSEVNS
                     ALLEYDASRPNDFYLEEFEFNTTPCINRVREIIDSVSQGRLTLITNLNPYMYNFAVPN
                     YKSNRFTAEDFKKYLVEDGGVSKIATTFYFLEGFEQSTNRESYNPNTLPVYSVFPSIN
                     YNPAKGILAYKETPANYDNWLQTNFDENTELAFDFSELLKLMDTMAKDKGIEFYSLHQ
                     SYSWFLPLFKLKEPTNEESEMTANLAISYGAKGLMYFAFNGNVTDNGFNSDYGHNGVD
                     FFQAGFLDSNLSVRINNAYGQNRWDYYKKYNSKLDKWGPYLMSFDNANRKSYIYRIPS
                     ERTEMTNSTFISELKTYPHVEFDPDPYPDIDNLPADTGSNVYLQAGFFNKPDDTGINK
                     YFMIVNRRCSPFVNDSSPSNVGGRRFVSMKINPSHLPGFNNWKI"
BASE COUNT         1257 a          531 c          656 g         1044 t
ORIGIN      
        1 aataacagac ctatagtgga tattactttt actgatagtc tgacagggta tgcaattgca
       61 agtaatagtg gaactgccga ttcaagttat attttgaaaa caactaacgg cggtgataac
      121 tggtatattt tatctataga atataagagt tattatgcaa tttcttttat cgataatgag
      181 acaggatatg taggtggtag tgatttaatg ggatattcat atctgataaa aacaacaaac
      241 ggcggtataa actggattcg ccagaactca ccaagtattt tgggattaac tgatttggca
      301 attcaaaatg aagacactat atggataaca gatccaaacg gtcttgacgg aggaatattc
      361 agaacaacta acggaggaac aaactggaca atacaacaac agccgggaag cagtattgaa
      421 aaaatctata tgtataataa aaatatcgga ttcgcctcat ctacgtcacg cctatataaa
      481 acaacgaatt ccggattgaa ctggaattta ataccgggac agaatgcata tcgcgatata
      541 tattttattg acagtctgaa cggatggaaa tcttatgaca gtatgaaaat aacaacggat
      601 ggcggattta catggaaacc gcagttaatt aaaagtcccg gaattccttt atttagtact
      661 attgcaacat tttcaaaaat cagtaatgat acaatatggg ctacaggttt ggtttacgag
      721 tttccaaatt tacaagttag aagtgtaata cacagaacaa caaatgccgg tagagattgg
      781 tattttcaaa tacctgacac gtctattcgt gttggtgttt ttagaacaaa ttttataaac
      841 acaatcactg gctggaatta tggaaattta agtggactat atactaacac cggcggagac
      901 agtattttta atcctatcac aagtataaat gaattcagca tcattgaaaa cccgagtttt
      961 tataaacttt tccaaaacta tccaaaccct tttaacccca caacaaagat cagttttgaa
     1021 ttacaaaaag ccggaaatgt caaattgacg gtttatgata ttcagggaaa atatattgaa
     1081 gtattagtaa atgaaaaaaa gcaaacagga aattatgaag ttgaattcga cggtagtaat
     1141 ttatcatcag gtgtttattt ttacagatta gaaatatcag gaaaagaaaa ttattctgat
     1201 acaaaacgaa tgatattatt aaagtaattt gaataataat ctattcgaaa aattgaaagc
     1261 cataaataaa aaatatataa agtatttttt aaaaattaaa ctttaccaat atgaaaagca
     1321 aaattgtttt cagtaccggg ttatttgttg tatcggtgtt tatgttttta agtttaacag
     1381 ctttggataa tccgcaggat aaatttttaa tagttgcaat gcacagcgga gtagatactt
     1441 cacccgtaaa ttatacaaga ttgaaggatt ctctgggatt gaacggatgg cacaggtata
     1501 accaatggtt taccggcgat gagataaatg gtaatatttc ctttatatct cccagagtac
     1561 agtacagagt ggatcaaaat aatacaagag atctgcgaac agtatttgga agacgaataa
     1621 cagattacag cgcatggggt cagagaagcg attatcagtg tgagaacgtt actgaaggtc
     1681 agccgtattg gttttattca tattataatt cggtttcaaa ttcgtggatc catgacattc
     1741 ccgacagcgg tcagactgtg aaatactgca aagtggatac aagtaacccc ggaagagatt
     1801 ccggatatat agtgcaggga ttaaaagcca accgcgaaca ggcaaacaga ttctggacgc
     1861 cataccagtc agatactttt agtgactggt atgtgaagcc aaaaataaga attccaacag
     1921 ggctgtctat aaatacaaag gtatgtcgta ttgagatatt ggattgggat agcagtatag
     1981 taaaaagcat tgaattaact gtaaatgaat ttaaagatgg aaatcaaaat tataatggaa
     2041 actatatgga tgaattttat tttcaaccta atgtactttc accaataaaa atagattctc
     2101 cgggactctg tccaggtcca gactcattac gaaagtattt ttgggattgg ggttcaacta
     2161 ctccattaaa cacaattaaa acagatttca gagttttctg gtatggtcaa tgcgatgtgt
     2221 ggatagaccg gataagggtt gagaatgagc aggcaaggtt actattcgaa aacacagata
     2281 ctaaagcaag aattgtttct gaagtcaaca gcgcattatt agaatatgac gcttctcgac
     2341 ctaatgattt ttatcttgag gaatttgagt ttaatacaac tccttgtata aacagagtaa
     2401 gggaaataat tgattctgta tctcagggaa ggctgactct tatcacaaat ctgaatccat
     2461 acatgtataa ttttgcagta ccgaattata aaagcaacag atttactgcg gaggacttta
     2521 aaaaatattt agtagaagac ggaggagtat caaaaattgc aactactttt tattttcttg
     2581 aaggatttga acaatctaca aacagagaat cttataatcc aaatacgctt cctgtatatt
     2641 ctgtatttcc ttctatcaat tataatcctg caaaaggtat tcttgcatac aaagaaacac
     2701 cggctaatta tgataactgg ctgcagacaa attttgatga aaacacagaa ttagcttttg
     2761 atttctctga gttattaaaa ttaatggata ctatggcaaa agataaagga attgaattct
     2821 attctctgca tcaatcctat tcatggtttc taccactttt taaattgaaa gagccaacca
     2881 atgaggaatc tgaaatgaca gcaaatcttg ctattagcta tggtgcaaag ggattaatgt
     2941 attttgcttt taacggaaat gtaacagata acggatttaa ctcagattat ggacacaatg
     3001 gagttgattt ttttcaagcc ggatttttag atagtaatct atctgtaaga ataaataatg
     3061 cttatgggca gaatagatgg gattattata aaaagtataa ttccaaatta gataaatggg
     3121 gaccatattt aatgagtttt gataatgcaa acaggaagtc ctatatctac cggattccat
     3181 cagaaagaac cgaaatgacg aatagtactt tcatcagcga actaaaaact tatcctcatg
     3241 tcgaatttga tcctgatcca tatcctgata tagataatct tccggcagat accggcagta
     3301 atgtatatct gcaggctgga ttttttaata aaccggacga tacaggaatt aataaatatt
     3361 ttatgatcgt aaacagaagg tgttcgcctt ttgtaaatga ttctagtccg tcaaatgtcg
     3421 gcggcagaag atttgtatca atgaagatca acccgtctca tctccccgga tttaataact
     3481 ggaaaatt
//
LOCUS       DAORVQ010000283         3483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_22534_length_3483_cov_5.805718, whole genome shotgun sequence.
ACCESSION   DAORVQ010000283 DAORVQ010000000
VERSION     DAORVQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22534_length_3483_cov_5.805718"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..476
                     /locus_tag="PLX80_14075"
     CDS             156..476
                     /locus_tag="PLX80_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY21965.1"
                     /translation="MAQNKKNEFSKSEIELADFAKAMSHPARVAIMKEIAKKNMCICG
                     EIVEVLPLAQSTVSQHLKELLNAGLIKGTVDGTKSCYCIDRKVFRKFAKSFEKFFLLT
                     QVQD"
     gene            564..1526
                     /gene="arsM"
                     /locus_tag="PLX80_14080"
     CDS             564..1526
                     /gene="arsM"
                     /locus_tag="PLX80_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HQY21966.1"
                     /translation="MIENLMTQKAETTKAAESNNSDCCGPDCCSDTESSSENAATEKS
                     TKLSNRDKSSAEIKDMVKKKYSEIALQSREMNASSCCGAGECSDEVYNIMTDDYESIK
                     GYNPDADLGLGCGLPTQFAKIKKGDTVLDLGSGAGNDCFVARSETGEAGKVIGVDFSD
                     AMIAKARENAEKLNFNNIEFRQGEIEDLPVNDDSVDVVVSNCVLNLVPEKLNAFSEMY
                     RVLKPGGHFSISDIVLIGELPQKIQKAAEFYAGCVSGAIQKNEYVSLIEKTGFRNIQI
                     QKEKVISIPDDILSKYLNKDEAASYKNSESKIYSITVFGEKSVD"
     gene            1712..2488
                     /locus_tag="PLX80_14085"
     CDS             1712..2488
                     /locus_tag="PLX80_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY21967.1"
                     /translation="MLRILITGCKGGIGFEAATKLLNKGHFVYVTVHEEESVSEVKEK
                     LKSFPNNYLVEKLDITEKTDREKILQWDLDVLVNNAGIGESGPLIEINIDRVRKVMET
                     NVFAALELTQIAVRKMIENKKGRVVIIGSMYGLVPTPFLAPYGMSKFALENVAYSLRG
                     ELKPFKIPVVMVNPGAYDTGFNLKNVNKKYEWQDKNGLYKNNMELIKKSEDQIFRLEV
                     KNTNGISDKVVKAVTAKNPKKRYYAPLFQWVLKSIGKHIS"
BASE COUNT         1205 a          493 c          693 g         1092 t
ORIGIN      
        1 agttaatagt taatagttaa tagttaatag ttaatagtta atagttaata gttgagaatt
       61 aaaattacaa atttcaaatt tcaaattacg agtttaggat tatgttcgga gaattggaat
      121 ttaaaattta aaatttaaaa tttaatatta aaatagtggc acaaaataag aaaaatgaat
      181 tcagtaaaag cgaaatagaa cttgcggatt ttgcaaaggc gatgagtcat cctgcacggg
      241 tagcaattat gaaagagatc gcaaaaaaaa acatgtgtat ctgcggggag attgttgaag
      301 tacttcctct tgcgcagtca accgtctcac agcatctgaa ggagttactg aacgcaggac
      361 ttatcaaagg tactgtagac ggtacgaaat cctgttactg tatcgacagg aaagtattta
      421 ggaaatttgc aaagagtttt gagaagtttt ttttattgac acaggtacag gattgaaata
      481 taaaaatctc cgaactgatc atccggaggt aaaacagaaa taaataaact aaaataattt
      541 attaaaaatt ttataataaa atcatgatag aaaatttaat gacacaaaaa gcagaaacaa
      601 ctaaagcagc agaaagtaat aattctgact gctgcggacc ggactgctgt tcggatactg
      661 aaagcagttc agaaaatgct gctacagaaa aatccacaaa attatcaaat cgggataaat
      721 cttctgctga gataaaagat atggtaaaaa agaaatacag tgagatcgct ttgcaaagca
      781 gagagatgaa tgcttcatca tgctgcggag cgggggaatg ttctgatgaa gtctataaca
      841 tcatgacaga tgattatgaa tcaattaaag gttataatcc cgatgcagat cttgggcttg
      901 gctgcggttt gccgacacag tttgcgaaaa taaaaaaagg tgacacagtc ctcgaccttg
      961 gatctggcgc aggtaatgac tgctttgttg ccaggtctga aacaggtgag gcaggaaaag
     1021 tgatcggtgt tgatttctca gatgctatga tagctaaggc gagagaaaat gctgagaagc
     1081 tgaactttaa taatattgaa ttcagacaag gggagataga agatcttcct gtgaatgatg
     1141 attcagttga tgttgttgtc agcaactgtg ttctgaatct tgttcctgaa aaactcaatg
     1201 ctttcagtga aatgtataga gtgctaaagc cgggcggaca ttttagtata tccgatattg
     1261 tactgattgg cgaacttccg caaaagatac agaaggcagc agagttttat gccggttgtg
     1321 tgtcaggagc aattcagaaa aatgaatatg taagtctgat agagaagaca ggattcagaa
     1381 atattcaaat acagaaggaa aaagttattt caattccgga tgacattctt agtaaatatc
     1441 tgaacaaaga tgaagctgca tcctataaaa attcagaatc gaagatctat agtattactg
     1501 tctttggtga gaagtcagtt gactgataaa atttattgta aattcaaaat cataatttcg
     1561 ccggtataga aagaatattg tgagtcagaa tatcaatctg actcattgtg ttttttagca
     1621 actgcttaaa cagtttcaga atttactaac aaaatggtaa taatatgttt attttctttt
     1681 ttaaatttat aaatataaat tcaaaaaaat tatgctcaga attctcataa ccggctgcaa
     1741 aggaggcatt ggatttgagg ctgccactaa acttctaaat aaaggtcact ttgtatatgt
     1801 tactgttcat gaagaagaat ctgtatcaga agttaaagaa aaattaaaat cctttccgaa
     1861 taattatctt gttgagaaac ttgatatcac agaaaaaact gaccgcgaaa aaatattaca
     1921 atgggatctt gatgtacttg taaacaatgc aggcattggt gaatcaggtc ctctcataga
     1981 aataaacatt gacagagtca ggaaggttat ggaaactaac gtctttgcag cactcgaatt
     2041 aacacagatc gccgttagaa aaatgataga aaataaaaaa ggaagagttg taataattgg
     2101 ttcaatgtat ggtttagtac cgacaccttt tttagctcct tatggaatga gtaagtttgc
     2161 acttgagaat gtggcttatt ctctcagagg agaactcaaa ccttttaaaa ttcctgttgt
     2221 aatggtaaat cctggtgctt atgatacagg ttttaatcta aagaatgtaa ataaaaaata
     2281 tgaatggcag gataagaacg gattatacaa aaataatatg gaactaataa aaaaatctga
     2341 agatcagatc ttcaggttag aagttaagaa cacaaacggt atttcagata aggttgtaaa
     2401 agcagtcact gcaaaaaatc ctaagaaaag atattatgct ccattatttc aatgggttct
     2461 gaaatcaatt ggaaagcata tttcctgaat taagatatgt caggtgagag aaaatttagc
     2521 agcaatttcg gagattgctt tgtatcttca ttcctggaaa ggaaatttca aagaaaatta
     2581 tttgaaatgt ctgtgaataa ctgattttaa aaactttgac cggaaatttt tttcggtttg
     2641 agaaatccca agaaatttat tttttgctct gattcctatg ttgctgcgta aatgctttat
     2701 acatagtata gttataatat atagtttggg cagatattga attttaccgg ctttaaacgt
     2761 ctttgaaaca aaaattactg catcgcaaag ttttcaagtg tgtttgcatt tgaaatgaat
     2821 tcaatcgaat ttcttacatc tgccttcgat ttcaaaacgg ggagcttcga attttctacg
     2881 aactccttct aatcaaaaat gaactgctta gaattttctg cgccggtatt tgaaattaaa
     2941 aacgtgctct ttcgaatttt ttatgagctc tttcgaatta aaaatgagga gcttcgaact
     3001 tccggtgagg acttttgaag agtcgttgtg cagcattgca tcagcattgc cgagcattgt
     3061 atcagaaatt cggtattttt gagagtatgc tgctgtcttc gtatgtgcag tgcggagaat
     3121 cgattctgtg tcgtgaacaa ttgtttcaaa aactttacac tttgtatgaa tttttccacg
     3181 attacattca aaaatttatg cttttgtaac tactttttgg gtctttgttt caaaaatttt
     3241 actctttgtt ttgattttga gtggatttga tttaaggtat aattttcaat ttttgaatgt
     3301 aaaattagtt cttctcctcc tcattttttt ggagggggac cggggggagg ttgcggagtg
     3361 gctttgagtg cggcaaactt ctcttatagt aactacaatt tttagtcgca gcttgggaaa
     3421 ttgcttcatc gcttcgctca tcgcaaaatc atatatatat tttacgggac cattaaaatt
     3481 gat
//
LOCUS       DAORVQ010000284         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_22834_length_3457_cov_5.047913, whole genome shotgun sequence.
ACCESSION   DAORVQ010000284 DAORVQ010000000
VERSION     DAORVQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22834_length_3457_cov_5.047913"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLX80_14090"
     CDS             <1..794
                     /locus_tag="PLX80_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQY21968.1"
                     /translation="EVNDEIPEFINQKIAQGEELKAYEKIGQNIASLIDNGSTLQMGI
                     GIIPDMVLQFLNDKKDLGVHTEMFSDNLINLIESGVINGERKTLLPGKVVSTFILGSK
                     KVFDYIDDNPVFEFRPTIFVNDPFVIARNDKMISINSALEVDLTGQVCADSIGHKNYS
                     GFGGQLDFIRGASRSEGGIPIIAFTSTAKGGTLSRISPFLKQGAGVTTTRADVHYIIT
                     EYGIADIYGKTVRERARSLIEIAHPDFREELEKYAYEVNFLRKNI"
     gene            838..1530
                     /gene="queC"
                     /locus_tag="PLX80_14095"
     CDS             838..1530
                     /gene="queC"
                     /locus_tag="PLX80_14095"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQY21969.1"
                     /translation="MIKIKPEAVVLISGGMDSALTAAIASIKYNLNFLHVNYGQKTQM
                     RELKAFKDVADHYNVKNKLVADISYLKSIGGSSLTDNKMKIKKADLKSKVIPSSYVPF
                     RNANILSIAVSWAEVINASRIFIGAVEEDSSGYPDCRKDFFDAYNKMISKGSKAGSKI
                     KVMTPIINLTKKEIVERSIRLNSPLHLTWSCYKENQISCGECDSCALRLRGFQSAGIT
                     DPLKYKTMINYS"
     gene            1541..2617
                     /locus_tag="PLX80_14100"
     CDS             1541..2617
                     /locus_tag="PLX80_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY21970.1"
                     /translation="MKKTDSSNFGLINVTCILILLTLSIFILKELESILLPLFVALLI
                     SFVFLPVYNYLNNKKLPAFLSLAIVILIILILANIVSVFIYTSINSFSSEFPLYEQKF
                     LHIYNDFIPKLNLTPEEETQLEQFFNIKKLFASGNLTTAISGILTSITALLGNFVLII
                     FYIIFIITESESIGKRFSLAYSEERNTSLRNTLGHIVIGLRDYISGKTLLSLMQAILI
                     GTILWICGVDFYVVWAFLFFFTDFIPNIGSLFASIFLGLFMALQFEAIIYPLVIIIIL
                     IVIQNLKGNVIEPKFFGSKLDLSPFLLFFSLVFWGYIWGILGMILSVPIMSMLKIILM
                     NIPSTKPIAILMSNNPEKTIFNKK"
     gene            2635..>3457
                     /locus_tag="PLX80_14105"
     CDS             2635..>3457
                     /locus_tag="PLX80_14105"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HQY21971.1"
                     /translation="MNISNKLRNWVDECAALCKPDKIHWCDGSDEEYQSLANMLIEKG
                     AIKKLNESKRPNSYYAVSDPTDVARVEDRTFICSEKEEDAGPTNNWKDPAEMKELLKG
                     IFDGCMKGRTMYVMPFSMGPLGSDIAHIGVEITDSPYVVMNMRIMTRIGSKVLDVLGD
                     GNFVHCLHSVGAPLADGQEDVNWPCNKEKYIVQFPEERSIWSFGSGYGGNALLGKKCF
                     ALRIASAMAKDEGWMAEHMLIVGITSPEGVKKYVAAAFPSACGKTNLAMLTPTIPG"
BASE COUNT         1201 a          496 c          662 g         1098 t
ORIGIN      
        1 tggaagtaaa tgatgagatt ccggaattta taaatcaaaa aatagctcag ggtgaagagc
       61 tgaaagctta tgaaaagata ggtcagaata ttgcttcact tatagataac ggatctacac
      121 tgcaaatggg aattggaata attcctgata tggtattaca gtttttaaat gacaaaaaag
      181 atcttggagt ccatacagaa atgttttctg ataatttgat caacctcatc gaatcgggag
      241 ttataaacgg agaaagaaaa actctcctgc ctggaaaggt tgtgtctaca tttattttgg
      301 gttcaaaaaa agtgtttgat tatatagatg acaatccggt tttcgaattc agaccgacta
      361 tttttgtcaa tgatcctttt gttattgcaa gaaatgataa aatgatttcc attaactctg
      421 cactggaagt agatcttaca ggtcaggtct gtgcagattc aattggtcat aagaattatt
      481 cgggatttgg aggacagctt gattttatca gaggggcatc cagaagtgaa ggtggtatac
      541 cgatcatagc atttacatct acagcaaaag gtggaacact gtcaagaata tcgccgtttt
      601 tgaaacaggg agcaggagtt acaaccacca gagctgatgt tcattacata attactgaat
      661 acggaattgc agatatatat ggtaagactg tcagggaaag agcaagaagt ttaattgaaa
      721 tagctcatcc ggattttcgg gaagaacttg aaaagtatgc atatgaagtt aattttttaa
      781 gaaagaatat ttgatgtttg acttcaaatt tcatgcataa ttaagtaatt tcaattaatg
      841 ataaagataa aacctgaagc tgtagtcctc atcagtggag gcatggacag tgcccttaca
      901 gcagcaattg ccagtataaa atacaatctg aattttctac atgtaaatta tggacagaag
      961 actcaaatga gagaactcaa ggcatttaaa gatgtggcag atcattacaa tgtaaaaaat
     1021 aaactcgttg ctgatattag ttatcttaag tcaataggag gttcctcact gactgacaat
     1081 aagatgaaga tcaaaaaagc tgacttaaaa agtaaagtaa ttccatcaag ttatgtcccc
     1141 tttcggaatg ctaatatttt atcaatcgca gtaagctggg ctgaagttat aaatgcatcc
     1201 agaatattta ttggtgcagt ggaagaagat tcaagcggtt atccggattg ccgaaaagat
     1261 ttttttgatg catataataa gatgatcagc aagggaagca aggcaggttc aaaaataaaa
     1321 gtaatgacgc caattattaa tctaactaaa aaagaaattg tagaaagatc gatcaggcta
     1381 aattctccac tgcatctcac ctggtcatgt tataaagaaa atcagatttc atgcggtgaa
     1441 tgtgacagtt gtgcattgag acttagagga tttcagtctg cgggaattac agatccctta
     1501 aagtataaaa caatgattaa ttattcctga atacgaaaat atgaaaaaaa ctgatagttc
     1561 taatttcgga ttaattaatg taacatgtat tctgatattg ttaacattat cgatctttat
     1621 tttaaaagag ctggagtcta tattacttcc tctttttgta gcattattaa tttcatttgt
     1681 ttttttacct gtttataatt atttaaataa taaaaaatta ccggcatttt tatcactggc
     1741 aattgtcatt ctgatcatat taatacttgc taatattgta agtgtcttta tatataccag
     1801 tataaattca ttctcttcgg aatttccttt atatgaacaa aagtttctac atatttataa
     1861 tgattttatt cccaaactta atcttactcc agaagaggaa acacagcttg aacaattttt
     1921 taatattaaa aaattatttg caagtgggaa tttaacaact gcgatatccg gaattctgac
     1981 aagtataact gcattgcttg gaaattttgt tctgatcatt ttttatataa tatttataat
     2041 tacagaatca gaaagcattg gaaagagatt ttcactggca tattcagaag aaagaaatac
     2101 ttcccttaga aatactttag gtcatattgt cattggttta agagattata tttcaggaaa
     2161 gacattactc agcctaatgc aggctatatt aataggcaca atattatgga tctgcggagt
     2221 agatttttat gtggtctggg cttttttatt ttttttcaca gattttattc caaatatagg
     2281 atcattattt gcatccatat ttctgggttt atttatggca cttcagtttg aagcaattat
     2341 atatccatta gtaatcatca taatattaat agtaatacaa aatttaaaag gtaatgtaat
     2401 tgaaccaaaa ttttttggtt caaaactcga cctgagtcca tttctattgt ttttttcact
     2461 ggttttctgg ggatatatct ggggaatatt aggaatgata ctttcggttc caataatgag
     2521 tatgctgaaa ataattttaa tgaacatacc atctacaaag ccaatagcaa ttttaatgag
     2581 taataatcct gagaaaacaa tttttaataa aaaataattt aacaaggaga taaaatgaat
     2641 atcagcaaca aattacgtaa ttgggttgac gaatgtgcag cgctttgtaa accggataag
     2701 atccactggt gtgacggctc ggatgaagaa tatcagagtc ttgcaaatat gcttatcgaa
     2761 aaaggtgcta ttaaaaaatt aaatgaaagt aaaagaccaa acagctatta tgctgtctca
     2821 gatcctacag atgtagcaag agtcgaagac aggactttta tctgttctga aaaagaagaa
     2881 gatgcaggtc ctacaaataa ctggaaagat cctgctgaga tgaaagaact tcttaaagga
     2941 atttttgacg gatgtatgaa aggcaggaca atgtatgtaa tgccattcag tatgggacca
     3001 cttggatcag atattgcaca cattggagtt gagataacag actcacctta tgtggtaatg
     3061 aatatgagaa ttatgacaag gatcggatct aaagttcttg atgtattagg cgacggtaat
     3121 tttgttcatt gtcttcattc agtcggagct ccacttgcag acggtcagga agacgttaac
     3181 tggccttgta ataaagagaa gtatattgtt cagtttcctg aagaaagaag tatatggtca
     3241 ttcggaagcg gctatggagg taatgcactt ttaggaaaaa aatgttttgc acttagaatt
     3301 gcatctgcaa tggcaaagga tgaaggatgg atggctgaac atatgttgat agttggaatt
     3361 acttctcctg aaggtgtaaa aaaatatgtc gcagcagctt ttccgagtgc atgcggaaag
     3421 acaaatcttg caatgctgac tcctactatt cccggct
//
LOCUS       DAORVQ010000285         3440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_23037_length_3440_cov_5.070310, whole genome shotgun sequence.
ACCESSION   DAORVQ010000285 DAORVQ010000000
VERSION     DAORVQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23037_length_3440_cov_5.070310"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3418)
                     /locus_tag="PLX80_14110"
     CDS             complement(<1..3418)
                     /locus_tag="PLX80_14110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY21972.1"
                     /translation="MLRSLSRTSDHKLFRNDYRQFYKEPARKGVSVIINIRDHKNFQP
                     VIFKQLSGQIIKSYVDPEVIVTVNNSDENKISLLSDNIKSEWKKYSNRELRLNILNFQ
                     EEHNSFKLKKAIENSANEFLIFFEDDEIPELDFLQNAVNLLRLNDHRYIAGGYDDIQF
                     ESKPPKWFERFSRYFFDHRAPLISPKNINSLNNIRFSNGLVINKKSAEKLIDTELSEL
                     DSTGNIFSTSKQIQENDFRLSSDLKLKKYFTKEEITWDNLRTIWRNNGIKYQIEFDKR
                     SEKESAKIPFQLRKTLNDLRKCGYKNLLSYNKTHENNLQILQIEYNLGKLDTLLNQKT
                     QNPKRQIRLIKKGARKKDHRYLLKLTNGISHKVERKGVSVIICCYNSAKLLPVTLEYM
                     SRQKTKNYVDWEVVVVNNNSSDNTSEVAAEEWRKYPECKGQLRIVNQPVPGLSAAREK
                     GIETSKYEYMVFCDDDNWLQENFVQNVVSVMRSNPEIGIAGSETQEVCEVEPPEWFHK
                     WKNWSYAVGEQFERAGDITWSRGFVWGAGMVLRREALNQLYNEGFKSLLTDRKGKELA
                     SGGDTELCYALRLAGWRIWYAPDLKLKHYMTAPRLKWDYFLKLWKGFGVSTAGLDTYL
                     NVIPSDMIEGNYVTVKKSWEENYKDTQQKLKDFGKDKVKNYQELPEGDPDVPLIVFNL
                     ARQEELKRIKDLYKKRIEETDKANWKRDFQQLRLKNKKYKVKSAEDKTIWPWVDQYDE
                     IDNENITEDLKISILTPSYLSEDKIEKAIQSVIRQGYKNYEHIVVDGGSKDNTVNILK
                     KYPHITWVSEKDKGQSDAMNRAFEMSTGDLICYLNSDDFFEPGAFAKVVKEFNLHKEI
                     DMVVGNLILDMKEGPMIIYPEKDYKKIMLSFYYSFPINPVSYFYKREVQEKIGKFPLD
                     NHYTMDYWFLLKAYKNFKIHKVEEILGTFVFHDVNKTSSADNLKNTHMTMIGHLKEND
                     PGKYLYYFYNYYNFKLFNKRSYRSQKSVTRYLRPKYIKSVRFGKDYSEHIYKKAYDKC
                     YHYSYLKALTLLMMSGVAYPPHIFKKSRRSLFYRSIMGHSLVEKFRNYYYKGIVKKYE
                     VKESLKNKLKLKSKPDTLILKSEADKKNVFSLMFSGKAGEYSDVIFNIAKD"
BASE COUNT          948 a          654 c          496 g         1342 t
ORIGIN      
        1 aatctttagc aatattaaat attacatctg aatattctcc tgcctttccg gaaaacatta
       61 atgaaaaaac atttttttta tctgcttctg actttaatat taatgtatcc ggtttacttt
      121 ttaacttaag cttattcttc agagattctt ttacctcata tttttttact atacctttgt
      181 aataataatt tctaaacttt tcaacaagtg aatgacccat tatggatctg taaaataaac
      241 tccttctgct ttttttaaaa atatgaggcg gatatgcaac acctgacatc attaataatg
      301 tcaaagcttt taaataactg taatgataac atttatcata agctttttta taaatatgtt
      361 cagaataatc ttttccaaaa cggacactct taatatattt aggtcttaaa tatcttgtaa
      421 cacttttctg acttctgtat gaccttttat taaataattt aaaattataa taattataaa
      481 agtaatataa atatttaccc ggatcgtttt ctttcagatg accgatcatt gtcatatgag
      541 tatttttgag attgtctgcc gatgaggtct tatttacatc atgaaaaacg aatgttccca
      601 gaatttcttc taccttatgt attttgaaat ttttataagc tttcaggaga aaccagtaat
      661 ccatagtgta atgattatca agaggaaatt ttcctatctt ttcctgtact tcccttttgt
      721 aaaaataact gacaggattt attgggaaag aataatagaa tgacagcatt atctttttat
      781 aatctttttc aggataaatg atcatcggtc cttctttcat atcaagtata agattgccaa
      841 ctaccatatc gatctcttta tgaagattga attcctttac aactttcgca aacgctcctg
      901 gttcgaaaaa atcatcagaa tttaaataac aaatcagatc acctgttgac atttcaaaag
      961 ctctgttcat tgcatctgat tgtcctttat ccttttccga aacccatgtg atatgcggat
     1021 atttttttaa gatatttact gtattatctt tagacccgcc gtcgactact atgtgctcat
     1081 agtttttgta accctgtctg atcacacttt gtatagcttt ttctatttta tcctctgaca
     1141 aatatgatgg agtaagaatg ctgatcttta gatcttcggt aatattttca ttatcaatct
     1201 catcgtactg atcaacccac ggccagattg tcttatcttc tgctgactta accttatact
     1261 ttttattttt taaccgtaac tgctgaaaat ctcttttcca gtttgctttg tctgtctctt
     1321 caatcctttt tttatataga tcttttatcc tctttaattc ttcctgtctt gccagattaa
     1381 aaactattaa cggtacgtca gggtctccct ccggcagttc ctgataattt ttaactttgt
     1441 cctttccaaa atctttcaac ttctgctgcg tgtctttgta attttcttcc cagctttttt
     1501 ttacggttac gtaatttcct tcaatcatat cagatggaat tacatttaaa tatgtatcaa
     1561 gtcctgctgt actgacgccg aatcctttcc atagcttaag gaaataatcc catttaagtc
     1621 tcggagctgt catgtaatgt ttgagcttca gatcaggtgc ataccagatc ctccagcccg
     1681 caagtctgag agcataacaa agttcagtat ctccgcctga tgcaagttct tttccttttc
     1741 tgtcagtgag aagtgatttg aaaccttcgt tgtataactg attcagagct tcccttcgaa
     1801 gcaccatacc agcgccccat acaaatcctc tcgaccatgt aatgtctccg gctctttcaa
     1861 attgttcgcc gactgcatag ctccagtttt tccatttgtg aaaccattca ggtggttcga
     1921 cttcacatac ttcctgcgtt tcgcttccgg caattccgat ctcaggattg cttctcatta
     1981 cactgacgac attttgtaca aaattttcct gaagccagtt gtcatcatcg cagaatacca
     2041 tgtattcata cttcgatgtt tctatacctt tttctcttgc agcgctcaag cctggtacag
     2101 gctgatttac aattctgagc tgtcctttgc actcaggata tttacgccat tcttccgctg
     2161 caacttcaga tgtgttgtct gatgaattat tgttgactac tactacttcc cagtcgacat
     2221 aattttttgt cttttgcctg ctcatatatt ccagtgttac aggcagaagt tttgcgctgt
     2281 tataacagca gatgatcact gatacacctt tcctctcgac cttatggctg atgccgtttg
     2341 tcagtttcag taaatatctg tgatcttttt ttcttgctcc tttcttgatt agtcttattt
     2401 gccttttggg gttttgggtt ttttggttca gcaatgtgtc tagttttccc aagttgtatt
     2461 ctatttgtaa gatttgtaaa ttattttcat gagttttatt ataacttaat aaatttttat
     2521 aaccgcattt tctaagatca ttaagtgttt ttcttagctg gaaaggaatt tttgctgatt
     2581 ctttttccga tcttttatca aattcaatct gatatttaat tccattgttg cgccaaattg
     2641 tccttagatt atcccaggtt atttcctctt ttgtaaaata ttttttcagc ttaaggtcag
     2701 aagataatcg gaagtcattt tcctgaattt gctttgatgt tgaaaaaata ttgccggtcg
     2761 aatccaattc acttaattct gtatcaatta atttttcagc agatttttta tttatgacaa
     2821 gtccgttact gaatctgata ttatttagag aattaatatt ttttggagaa atcagaggag
     2881 cccggtggtc aaagaaatat ctggaaaatc tttcaaacca ttttggcggc ttggattcga
     2941 attgaatatc atcataacct ccggctatat atcgatgatc gtttaatcgc aggaggttta
     3001 ctgcattttg cagaaaatcc aactcaggga tttcatcatc ttcaaaaaat atcagaaatt
     3061 catttgcaga attctcaata gccttcttta atttaaaaga attatgttct tcctgaaaat
     3121 tcaggatatt taatctgagc tctctgttac tgtatttttt ccattcgctt tttatattat
     3181 cacttaataa acttatttta ttttcatcag aattattaac agttactatt acttcaggat
     3241 caacatatga ttttatgatc tgtcccgaaa gttgtttgaa aattaccggc tgaaaatttt
     3301 tatgatcacg gatatttata ataacagata caccttttct tgccggctct ttataaaatt
     3361 gcctgtaatc atttctgaat agtttatgat cagatgttct tgacagagat cttagcaaga
     3421 aagtctgttc aattttattg
//
LOCUS       DAORVQ010000286         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_23140_length_3431_cov_4.696386, whole genome shotgun sequence.
ACCESSION   DAORVQ010000286 DAORVQ010000000
VERSION     DAORVQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23140_length_3431_cov_4.696386"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1068)
                     /locus_tag="PLX80_14115"
     CDS             complement(151..1068)
                     /locus_tag="PLX80_14115"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQY21973.1"
                     /translation="MKKKINITSLESVFKSNSDKAAIGIFGNTAKPVVAELLKYITDI
                     LEKNNLNYFIEKDLADFGKFNNRSKISNTSEIIYKSDYVFSLGGDGTFLNTAKLVNNK
                     NIPILGINLGRLGFFSEITPKEFESFIPKLISGKFKITEQVVIKAFSGGRKFLFGLND
                     IVIDKNDSIRTMETETFYNNEKVVNIISDGIIISTPAGSTGYSMSCNGPIVNPESKVF
                     IITPISPHTLNVRPIIIPDDGEIKIKISNIGKARIIADGQNTYKSVTPSEVTLQKADF
                     TIKVIKKNSSTYFDTLKKKLFWNADKRHG"
     gene            complement(1052..1483)
                     /locus_tag="PLX80_14120"
     CDS             complement(1052..1483)
                     /locus_tag="PLX80_14120"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HQY21974.1"
                     /translation="MNEDKNTQLFMALVYSLQMQTMIHLGKIQNPGTGEIEENLDAAQ
                     ATIDMLDMLNEKTKNNRSDDETNFIKNCLSDLKLNFTSEKIKKESESKNISTEEKSQD
                     DDTTATEDNSDSSDQVIEENQAGEAKEVKADSDKTEDEKKD"
     gene            complement(1616..2320)
                     /locus_tag="PLX80_14125"
     CDS             complement(1616..2320)
                     /locus_tag="PLX80_14125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY21975.1"
                     /translation="MNPQYSEDHLNHYYSTYIRDESFKEDNLIEAQSYCLSIVESQVP
                     DKGNLLELGSGNGYLLDIAKLNGWKPFGHEIDCISAGKLSERLGLEILCGDFLTLEID
                     KTFDAVIMLHVLEHLKDPVEHLNKISGLLNKGGVLFIALPNIESRSGLVKLGLEKLKI
                     KKKNIAAYYDTEHHLWYYTPATIRKLLEKNFDVITVYSGDRFNPVRGKLNTIIEEKIF
                     SKLLWHSSMGILAVKK"
     gene            complement(2605..>3431)
                     /locus_tag="PLX80_14130"
     CDS             complement(2605..>3431)
                     /locus_tag="PLX80_14130"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY21976.1"
                     /translation="LIIITGIVSSVYGCFNFLTGSVERATSLTGGYYTLGIYLTLVLS
                     ILLFTKKGKNLIPTNIGWYFSVLIIISGIICTLNRMHWVLMVFLIVISFIINKDKILI
                     TGFILLVILSILIIPDIMNRFDLLINLMSNTSDRNIIYQAALDMVFNRPIFGHGLATF
                     KEIFPSFDKVGDGAINSWHNDFIQVYMESGIVGLFSYVFLIISVLKIGYINIKRFFNN
                     QFYKSISIGILISTTVLMITGGFFDPITSIIIFVMLGLLSLISSNSESIIETEKLN"
BASE COUNT         1122 a          549 c          476 g         1284 t
ORIGIN      
        1 gttaatagtt aatagttaat agttaatagt taatagttaa tagttaatag ttaagataac
       61 ttttcacttt cactttcact tttcactttt cacttttcac ttttcactca gcaaaacact
      121 gcacattgaa cattaaaaat tgaacattaa tcacccatgt cttttatccg cattccagaa
      181 caattttttc tttagagtat caaagtaagt tgatgaattt ttctttatta ctttaatagt
      241 aaagtctgct ttttgcaaag taacttcaga tggggttact gatttataag tattctgacc
      301 gtcagcaatg atccgtgctt ttccgatatt tgaaatttta attttaattt ctccgtcatc
      361 aggaattatg atcggtctga catttaatgt atgaggggaa attggtgtga taataaatac
      421 tttactttcg ggatttacaa taggaccatt acaagacatt gaatatcctg tggatcctgc
      481 aggtgtagag ataataattc cgtctgaaat aatattcact actttttcat tgttataaaa
      541 ggtttcggtt tccattgtcc tgatggaatc atttttatct attacaatat cattaagacc
      601 aaatagaaat tttctgccgc ccgaaaatgc ttttataaca acctgttcag taatcttaaa
      661 cttaccgctg atcagttttg gaataaaact ttcaaactct tttggtgtga tctctgaaaa
      721 aaatccaagt cttccgagat tgattccgag aataggtata tttttattgt taactaattt
      781 tgcagtatta agaaaagtac catcacctcc taatgaaaat acataatcag atttataaat
      841 tatctcagat gtgttcgata tttttgatct attattgaat ttcccaaaat cagcaagatc
      901 tttttcaata aaataattta aattattctt ctcaagaatg tctgtaatat atttcagaag
      961 ttcagctaca acaggtttgg ctgtattccc aaaaatacct attgcagctt tatctgagtt
     1021 acttttaaaa acagattcta atgaggttat attaatcttt tttttcatct tcagttttgt
     1081 ctgaatcagc tttaacttct tttgcttcac cggcttgatt ttcctcaatt acttgatctg
     1141 aagaatctga attatcttca gttgctgttg tatcatcatc ctgagatttt tcttcagtag
     1201 aaatattttt actttctgat tcttttttaa ttttttcaga agtgaaattc agtttaagat
     1261 ctgatagaca atttttaatg aaattggttt catcgtctga acgattgttt tttgtttttt
     1321 cattcagcat atccagcatg tcgattgttg cctgagctgc gtcaagattc tcttcgattt
     1381 ctcctgtacc agggttttga attttaccga gatgtatcat tgtctgcatc tgtaaactgt
     1441 aaacaagtgc cataaacagt tgtgtgtttt tatcttcgtt cattttttaa tttaattatt
     1501 taataatatt ttgagagtat gaatataagg attatgggaa tttatttgga ttcagaaaaa
     1561 tggtcatgtg agatgtaata ttgtttttaa catcatatct ttaattcaaa taaatttatt
     1621 tttttaccgc taaaattccc attgaagaat gccagagtaa ttttgaaaat attttttctt
     1681 ctataatggt atttagtttg ccgcgtaccg gattaaatct gtctccgctg tacacagtaa
     1741 tcacatcaaa gtttttttca agaagctttc tgatagtggc aggggtatag taccataaat
     1801 gatgttcagt atcatagtat gcagctatat ttttcttttt aatttttaat ttttccaatc
     1861 ccaattttac cagtccggat cttgattcta tatttggcag agcaataaaa agtacccctc
     1921 ctttattgag caatcctgaa attttattta aatgttctac aggatctttc aaatgctcaa
     1981 ggacatgcag cattatcaca gcatcaaagg ttttatcaat ttccagagta agaaaatctc
     2041 cgcacaaaat ttccaatcct aacctctcac ttagtttgcc tgcactaatg cagtctattt
     2101 catgaccaaa aggtttccag ccattgagtt ttgcaatatc cagtaaatat ccattccctg
     2161 aaccaagctc aagaagatta cctttatccg gaacttgtga ttcaacaatt gacaaacaat
     2221 atgattgcgc ttcaataagg ttatcctctt taaaagattc atcccgaata taagtagaat
     2281 aataatggtt taaatgatct tcagaatact gaggattcat aaattgagtt ttacaatctc
     2341 tgcatttaaa aatttgtgca ccgttttctg attcgtggaa tttgtatata tttcttgagc
     2401 cacaaatttc acagattatt ttcggcggat catatttaat caaaattgat tttattaatt
     2461 taacaaaatt atattttata caaaaaattc tgaactatat acattcagat ttaaacttga
     2521 aatatttaac ttccgctaat taatataatt tacattaaat tatttaatga aaattcattt
     2581 caatatttct ctgagttaag aaactcaatt taatttttct gtttcaatta ttgattcact
     2641 attcgaagag atcaatgata ataaacctaa catgacgaat ataattatgg atgtgatcgg
     2701 gtcaaaaaaa ccaccggtaa tcattaaaac tgttgtactt ataagtattc cgatagatat
     2761 ggatttataa aactgattat taaaaaatct tttaatattt atatatccga tctttaaaac
     2821 tgatataata agaaacacat aagaaaataa tcccactatt ccgctttcca tatatacctg
     2881 aatgaaatcg ttatgccagc tgttgattgc accgtcacca actttatcaa aactcggaaa
     2941 aatttcttta aaggttgcaa gtccatgtcc aaagattggt ctgttaaaaa ccatgtctaa
     3001 tgctgcctga tatataatat ttctgtcact tgtatttgac attagattta tcagcaaatc
     3061 aaatcgattc attatatcag ggataattaa tattgaaaga attactaata aaatgaaacc
     3121 tgtgatcaat attttatctt tgttgataat aaatgaaatg actattaaga aaaccattaa
     3181 gacccagtgc attctattca aggtgcagat gatccctgaa ataattatca gaactgaaaa
     3241 ataccatccg atgtttgttg ggattaaatt ttttcctttc tttgtgaata aaagaattga
     3301 aagaaccagg gtcagataaa ttccaagggt gtaatatcct cccgtcaatg aagttgctct
     3361 ttcgactgaa ccggttaaaa aattaaaaca tccgtaaaca gaagaaacta ttcctgtaat
     3421 aattattaac c
//
LOCUS       DAORVQ010000287         3384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_23686_length_3384_cov_7.351457, whole genome shotgun sequence.
ACCESSION   DAORVQ010000287 DAORVQ010000000
VERSION     DAORVQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23686_length_3384_cov_7.351457"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3236
                     /locus_tag="PLX80_14135"
     CDS             <1..3236
                     /locus_tag="PLX80_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21977.1"
                     /translation="SLPVFISICAFMILAFGLSDKNSDRDPKIISDRNNIPYQTAAVD
                     SKKMDGNNISTWYRNNGSLNRNPSTGNAGFEWPKGSGLFARFASGLWIGAVVGDDTLV
                     VVADYSSEYLPGYIDQNGNPQGKDDPQYRIYKINKGDIFSNDYQNWPVNQGAHSDIND
                     KPFLMGDQTMFYSYTDGYPESHGNNSGQTSPLKAQILQTNFCVFKIPGFLNSMIITEM
                     RIINRNDLPWTKCFFGLFTDDAVGVPTDDAVGCDTILNLGYTYNFNPIDGDYGFSPPA
                     VGFMFLKSAESQSQSDTVKYYSPPMSNNLIIKPGYKDLGMTAFNLYASGNPIIGEPSN
                     YIQTYLNLQGIRRNGTTWINPFTNQATKFAFSGDPFYQNGWIQTEGYDRRFLISTGPT
                     IVNPGDTQTIIFAQIISNSINNLASVNSLKFHSQYLKNFYDYNFNISISAKSPDLTSY
                     SYGNNKIYLSWNDTAEKVNYENKFTGSYYNFQGYNIYQISSYSPHPSESDTLLIKTFD
                     KTDGIKDIRDSIYLEEYQSFTYGIVQKGSDNGISRYIELDKDSLGNQNFINGAEYKYC
                     VTAYYFDSLAGPFSLPKVLESPKIVLKVIPQNIASGTTIYYSLSDTVSTDQKDNASTP
                     VVIDPVKLLNATYSSVFGMNNNIMNWTLTRNFSGSSDVLFQNVENFTGKQDTAKTADG
                     LMFIHKLIRDSGIVRDPDDNFYKNNNIPTNSIQRAWSYEPAENNWFRGPDTSAVITAK
                     VFTNRQFDSRSLGMSFPTQARFNNFRSRVIANGKYFIQGTGTNTLPMGGPLRKIKIVF
                     GENSKAYRYAPPVNVLLTDTNLLVTPYSDFADVPFSVYAVDELDSTQGNPRRLNIGFV
                     DADANGQWDPDDSPLGKYQLTYIFASDYSEVINENYVTRSGQAINPGIGSPVFGFPSL
                     DIMYAWLPRKKSASSTWSDGDILTVTPYRITRADFVPGFPLKYSWTINGSTFNNSELA
                     ASQINNIKAYPNPYYGFSSLEFNDAGEKIIYFSNLPGVCDIFIYSLDGALVKQISRNT
                     SDPNSSLSKWDIKNDDGKYVASGMYVVYIDCKAAGAKTLKIAVFQSR"
BASE COUNT         1114 a          578 c          672 g         1020 t
ORIGIN      
        1 ttagtttacc ggtatttatt tcgatttgtg cattcatgat cttagcattc gggctgagtg
       61 ataaaaattc tgatagggat ccgaaaataa tttcagacag aaataatatt ccatatcaga
      121 ctgcagctgt agattcaaaa aaaatggatg gaaataatat aagtacatgg tacagaaata
      181 acggcagtct taaccgaaat ccttcaacag gaaatgcagg ttttgaatgg cctaaaggca
      241 gcgggttatt tgcgagattt gcttcaggcc tttggatcgg agcagttgtt ggagatgata
      301 cattagtagt ggttgcagat tacagctctg aatatcttcc aggatatatt gatcaaaatg
      361 gaaatccgca ggggaaagat gatccgcaat acaggatata taagatcaat aaaggagata
      421 tattcagtaa cgattatcag aactggccag taaatcaggg tgctcattca gatataaacg
      481 acaagccgtt tcttatggga gatcagacaa tgttctattc ttatactgac ggatacccgg
      541 aatctcatgg aaacaattca ggtcagactt cacctttgaa agcacagata cttcagacaa
      601 acttttgtgt atttaaaatt cctgggtttt taaacagtat gatcattaca gaaatgcgga
      661 tcataaacag aaatgatctt ccctggacga aatgcttttt tggattattc acagatgatg
      721 ctgtcggtgt gccaacagat gacgccgttg gatgtgatac aattttgaat ttaggatata
      781 cttataattt taatccaatt gacggagatt atggattttc accaccggct gtcggattta
      841 tgtttctaaa aagcgcggaa agtcaaagtc aaagcgatac tgttaaatat tatagtccac
      901 ctatgagcaa taatttaata ataaagcccg gctataaaga tcttggtatg actgctttta
      961 atttgtatgc aagcggtaat ccgattatag gagaaccaag taattacata cagacttatt
     1021 tgaatcttca gggtatcaga aggaatggaa caacttggat aaatcctttc acaaatcagg
     1081 ctacaaaatt tgctttctca ggtgaccctt tttatcaaaa tggctggata caaactgaag
     1141 gttatgacag aagattttta ataagtaccg gtccgacaat tgtaaatccg ggcgatactc
     1201 agacaattat atttgcacag ataatttcaa acagtatcaa caatcttgct agtgttaact
     1261 ctttgaaatt tcactcacaa tatcttaaaa atttttatga ttacaatttc aatatatcaa
     1321 tatcagcaaa atctcctgat ctcacttcat attcatacgg aaacaataaa atctatttat
     1381 catggaacga tactgccgag aaagttaatt atgaaaataa attcaccggt tcatattaca
     1441 attttcaggg atacaatatt tatcagataa gctcttactc acctcatccg tcagaaagtg
     1501 atactttgct gataaaaact tttgataaga cagacggaat aaaagatatc cgcgacagta
     1561 tttatctgga agaatatcag agctttactt atgggattgt tcagaaaggc agtgataatg
     1621 gtatctcaag atatattgaa cttgataagg attcactcgg caatcagaat tttataaacg
     1681 gagctgagta taaatattgt gtgacggcat attactttga ttcacttgca ggtcccttca
     1741 gtctgccaaa agtacttgag tcgcctaaga ttgttctcaa ggtcatacct cagaatatag
     1801 cttcaggtac taccatctat tattcattaa gtgatactgt atcaactgat cagaaagaca
     1861 atgcttccac acctgtagtt attgatcctg taaaactttt gaatgctact tacagctctg
     1921 tttttggaat gaacaacaac attatgaact ggacactgac tagaaatttt agcggaagca
     1981 gtgatgtact ttttcagaat gttgagaatt ttacaggtaa gcaggataca gcaaagactg
     2041 ctgacggact gatgttcatt cataaattaa ttcgtgattc gggaattgtc agggatcctg
     2101 atgataattt ttataaaaat aataacatcc cgaccaattc aattcagaga gcatggagct
     2161 atgaacctgc tgaaaacaac tggttcagag ggccggatac atcagcagta attactgcaa
     2221 aagtatttac aaacagacaa ttcgacagcc gcagtcttgg aatgagtttt ccgacccagg
     2281 caagatttaa taatttcaga tcgagagtga ttgcgaatgg aaaatatttc atacaaggaa
     2341 caggaacgaa tactctgcca atgggtggtc ctctgagaaa aattaaaatt gtatttggtg
     2401 aaaattccaa agcttacaga tatgctccgc cggtaaatgt cttgcttact gatacaaatt
     2461 tacttgtcac accttattca gattttgcag atgttccttt tagtgtgtat gcagtcgatg
     2521 aactcgactc tacccaggga aatccaagac gtctgaacat tggatttgta gatgctgacg
     2581 caaatggtca gtgggatccg gatgattcac ctttggggaa atatcaactt acatatattt
     2641 ttgcttcaga ctatagtgaa gtcataaatg aaaattatgt aacgagatca ggtcaggcaa
     2701 taaatccggg tataggaagt cccgtctttg gatttccgtc acttgatata atgtatgcct
     2761 ggctgccgag aaaaaagtct gcgtcaagta cgtggtcaga cggtgatatc cttacagtca
     2821 ctccatatag aattacaaga gctgatttcg tgccgggctt tccactgaaa tattcatgga
     2881 caataaacgg atctacattt aataacagtg aactagcagc atctcagatt aataatatca
     2941 aagcttatcc gaatccttac tatggatttt cttcattgga atttaatgat gcaggtgaaa
     3001 agataattta cttttctaat ctgcctggtg tgtgtgacat ttttatttac agtctggacg
     3061 gagctttggt aaaacagatc agcagaaata cgagtgaccc gaacagttca ctgtcgaagt
     3121 gggatataaa aaatgatgac ggtaaatatg ttgcaagcgg aatgtatgta gtttatatag
     3181 actgcaaagc tgccggtgca aagactttga agatagcggt gtttcagagc cggtgacctc
     3241 taagactttt tgacctctaa ggctcaaagg ctcgaattct caaaggagat atgactttat
     3301 gtatttaaac tttgagtcct tgaggctttg agtctttgag acagtaccaa agttttcata
     3361 caagttgaat atttggaaat gagc
//
LOCUS       DAORVQ010000288         3384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_23687_length_3384_cov_5.141484, whole genome shotgun sequence.
ACCESSION   DAORVQ010000288 DAORVQ010000000
VERSION     DAORVQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23687_length_3384_cov_5.141484"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLX80_14140"
     CDS             complement(<1..524)
                     /locus_tag="PLX80_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQY21978.1"
                     /translation="MAKNYKERLEEHCKNSKWDFSDVRALFINCTLKRSPQISHTEGL
                     IDISKTILEKNKVSAEVIRASDHEIAPGVYPDMTEHGWEKDEWPEIYKKVKAADILVL
                     CSPIWLGEKSSICQKVIERLYGQSGELNDQGQYSYYGKAGGCLITGNEDGIKHCAMGI
                     LYSLQHLGYTIPPQA"
     gene            complement(719..1951)
                     /locus_tag="PLX80_14145"
     CDS             complement(719..1951)
                     /locus_tag="PLX80_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQY21979.1"
                     /translation="MTAAVIITICLLLLLAYVFDITASKTRIPSIILLLVLGFGLKQI
                     TDLAGYPLPNLQPILPILGTIGLILIVLEGSLELDIDRSKLKFVGTTFLLALLSILVM
                     SFGLAYSFFYFFDYSLKYSLTNAIPMCIISSSIAIPSARYLLAKDKEFVTYESSLSDI
                     IGVIFFNFITLNESIGTYSFGFFILELLIMFIVSFLATMALSFLIAKIRHHFKFVPII
                     ILVVLIYTISKMFHLPALIFILIFGLVLQNLDKLKGFHFIQILNPDILDLEVKKFMEV
                     TTEFSFLIRALFFLLFGFLIEFDDLINIDTLPWAGGIVISVFVIRAIFLKVLKLRITP
                     LLYIAPRGLITILLFLSIPINQKIAIVNNALLIQVIVLCAIIMMIGLIFSKNIQPAPL
                     LRSEEENDPERDKVFDMI"
     gene            complement(2477..3061)
                     /locus_tag="PLX80_14150"
     CDS             complement(2477..3061)
                     /locus_tag="PLX80_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQY21980.1"
                     /translation="MTDNNKRPRQAKILRIFRKVHRTIGAILFIFFFFISITGLLLGW
                     KKNSGGVILPKSYTGTSTDLKDWLPIDSLHKISDSIILDSVSADLSLELERIDMRKDK
                     GMLKFVYIDGFYGIQLDGATGKLLHIERRRSDFIENLHDGSILDYFFETTSEQIKLVY
                     TSVMGLSLLTFTVTGFWLWYGPKRMRNRPGKKQN"
BASE COUNT         1162 a          567 c          545 g         1110 t
ORIGIN      
        1 gcctgtggag gaatagtgta tccgagatgc tgtagtgaat aaagaattcc catggcgcag
       61 tgtttgatcc cgtcttcatt acctgtgatc agacatcctc cggcttttcc ataataagag
      121 tattgaccct gatcatttaa ttcgcctgac tgtccataaa gtctttctat gactttctga
      181 caaatcgaag atttctcacc gagccagatt ggactgcaca atacaagtat gtcagcagcc
      241 tttacttttt tatagatctc aggccattca tctttttccc aaccatgctc ggtcatatcc
      301 ggatagactc cgggagcgat ttcgtgatcg gatgctctga tgacttctgc gcttactttg
      361 ttcttttcta aaatagtttt ggaaatgtcg ataagaccct ctgtgtgaga aatttgcgga
      421 gatcttttca atgtgcagtt aataaataat gctctgacat cggaaaaatc ccatttggaa
      481 tttttgcaat gctcttccag tctttctttg taattttttg ccatgataat ttttaaataa
      541 atatagtaat tatttcatta taagtaacat gaaaaaaatt acagtactgc ttttttttaa
      601 aaaattattc ttagaatttt cgcagaagca atttgaatac gaagttagat ttgacatttg
      661 catttacaat aaaaagcaaa agaaaatttc agattatctt taagatgata ttcagagatc
      721 agatcatgtc aaagacttta tctctttccg gatcattttc ttcttcactc cttaacaaag
      781 gtgcaggctg aatattttta ctgaatataa gacctatcat cattatgata gcacataaaa
      841 cgatcacctg tatcagcagt gcattgttca caatagctat tttctgattt atcggaattg
      901 aaagaaataa caatattgta ataagtccgc gcggtgcaat atacagaaga ggtgtaattc
      961 ttaattttaa tacttttaaa aatattgctc tgataacgaa tactgaaatt acaattcctc
     1021 ctgcccacgg taatgtgtca atatttatca gatcatcaaa ctctatcaaa aagccgaaca
     1081 gaagaaagaa caatgctctt atcagaaaag aaaattctgt agttacttcc ataaactttt
     1141 tcacttcaag atccagtatg tcaggattca gtatttgtat aaaatgaaaa ccttttagtt
     1201 tgtccagatt ctgaagtaca agaccaaata ttaatataaa aataagtgcc ggcaaatgaa
     1261 acatttttga aattgtgtat atcaaaacta caagtattat aatcggaaca aatttaaagt
     1321 gatgtcttat ctttgcaata agaaaagaca aagccattgt agctaaaaaa gaaactataa
     1381 acattatcag cagctcaagt ataaagaatc cgaatgaata tgttccgatg gactcattaa
     1441 gtgttataaa gttaaaaaag atcacaccaa taatatcaga aagactgctt tcataagtca
     1501 caaattcttt atcctttgcc aggagatacc gcgcgcttgg aatagcaata gaactgctga
     1561 ttatgcacat cggaattgca tttgtcagtg agtacttcag ggagtaatca aaaaaataaa
     1621 agaaactgta tgcaagacca aatgacatta ccaagatcga cagcagtgca agaaggaaag
     1681 tagtacccac aaattttaac tttgatctgt ctatatcaag ctccagtgat ccttcgagca
     1741 cgataagtat cagtccaatg gttccgagta tagggagtat cggctgaaga tttggcagtg
     1801 ggtatccggc taaatctgtt atttgtttta agccgaagcc aagaaccaat aataaaataa
     1861 tagaggggat ccttgttttt gacgcagtaa tgtcaaacac atatgcaagt agtaaaagaa
     1921 gacaaattgt tattattaca gctgcggtca ttaaagaatt ttatttttcg gtatatagtt
     1981 ttttcgtgaa ttttaaattt tttgaaattc cggaaactta aataatttaa agaataagat
     2041 cagttgattc tgaaaagatc tgaaaaaatt taataaactt cctgacaaac ttctttcaaa
     2101 aatttacaaa ataaattatt tacaaatttg ctattcttga atgcttattc aaggtataac
     2161 ctactttaca tttaggaata agaaaagctg aaaataaaat gatgtttgaa atgtcctgat
     2221 gaaaatttac cggttgaaga aaatttaatc cttattcttc tatcctgaat gcggaatatt
     2281 tttacatctg taatgttggg aaaaatatgt cttattatac ctttgaatat aggtacaatt
     2341 acagttggaa ttatggggtt aaatacaggg aagtatgaca actactgctt tgataaatat
     2401 gaaaaagaaa aaacagataa aataaaaaag atacttaaag ttaaaagata aaatttttat
     2461 cagatcaatc tgcattttaa ttctgtttct taccgggtct attcctcatt ctctttggtc
     2521 cataccaaag ccagaatccg gtgacagtaa aagtcaaaag agaaagtccc attacagaag
     2581 tataaaccag tttgatctgc tcgctcgtag tttcaaaaaa ataatcaaga atggaaccgt
     2641 catgtaaatt ttcaataaaa tccgaccgcc ttctttctat gtgtaaaagt tttccggtcg
     2701 ctccgtccag ctgaatgccg taaaagccgt ctatgtatac aaacttaagc atacccttat
     2761 cttttctcat gtctattctt tccagttcga gagatagatc agctgacact gaatccaata
     2821 taatgctgtc ggatatttta tgaagactgt ctatcggcag ccagtctttg agatcggtag
     2881 atgttcctgt gtaagatttt ggcaaaataa ctccgccgct gttttttttc cagccgagta
     2941 agagacctgt tatcgaaata aaaaagaaaa aaataaaaag aatcgcacct attgtcctgt
     3001 gaacctttct gaatatacgt aatatctttg cctgtctcgg tcttttgttg ttgtctgtaa
     3061 taatatggta tatttaaaat agtaaaataa atgtttatga aaattcaaat tatctgataa
     3121 aaatattatt tatattctat tcataataaa tttaataaac ttaataaatt taaaaatgta
     3181 cttcattttt atatttagta tcattttaca ataaattcca gctgcaaaaa aatctaattt
     3241 gtagatctct caaatgcctc tctgtgaatc attgcatttt ttccggatgg ccgtcttcat
     3301 ctttagtgaa acctgtaatc gcatctttat ctaccgatct gacatgcaga tctctctgcg
     3361 gaaacgggat ttcaatgccg gctt
//
LOCUS       DAORVQ010000289         3283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_24974_length_3283_cov_5.501239, whole genome shotgun sequence.
ACCESSION   DAORVQ010000289 DAORVQ010000000
VERSION     DAORVQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24974_length_3283_cov_5.501239"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1406
                     /locus_tag="PLX80_14155"
     CDS             27..1406
                     /locus_tag="PLX80_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY21981.1"
                     /translation="MKAIKKIFDPNKLSTLLFYSSILSFIIAFNFAHNPPGGWTQQYM
                     PNLNNRPLVGLEFTDSLIGYSILSSQATDTSFLIKSTNGGDDWNTIFFEFRKYNCFQF
                     LNNSIGFIGGGDLEFGQSYLIKTNNGGENWIRLNTPNSSSISSISVLSEDSIWISVAS
                     GFDGGIFRTTNGGMNWTYQGLRSNGIERIYMYNGNVGFASSQNYMVRTTNSGINWDII
                     QGQNGYRDISFIDSLTGWKSYGDTMKFTTNGGLNWSSQKLPIGGKIEVSIMSNFDNIN
                     NDTLWGCGGWVRYPNNQFRGILYRSTNKGLIWEYQIPDTSINIGIYEKIDFVSNSIGW
                     SYIQYPNYITGIHTTVGGDTSFITNIKSSPIISNHDFKLFRNYPNPFNPKTKISYELK
                     QKAEVTIKVFDIRGNEIRTLVNANIFEGYNEVEFDGTRFSSGIYFYRMEVKTSKEYFT
                     DSGKMILLK"
     gene            1468..3264
                     /locus_tag="PLX80_14160"
     CDS             1468..3264
                     /locus_tag="PLX80_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21982.1"
                     /translation="MKKKILLSFVLMTISFLMFISLTGIDNPDEKFLIVAMHSGIDST
                     SANYSRIKDSLGMNGWHKYNQWFEHDEITSDISFLSPRVQYRVNENNTKDLRTVFGRR
                     ITDYTGWGQRSDYQCEVIPYGNDYWFYSYDTSVINTWINDIQDSNATVKYCDAIESSP
                     GSHASYIIKGLKANREQANRRWTPWQSDTYSSWYIKPKIRIPTGLSPNTLVCTIEILD
                     WDGTIVRSVVLKANNFERSSLSPYQGNYLDEFYFVPNQNASPIKTDSTELCPGSNLKD
                     FTNWGDTNAIIKTDFRVYWHGECDMWIDRIRVENEQAIQLFQEGGDADLRIRSEVSSA
                     LLDYDPSRPNHFYLEEFEFNTTPCIKKVREIIEEESQGKLTFIANLNPYMYNFHVPNY
                     VSHPFTADEFKKYFIDPTTGGGVKIAAATFYFLEGFEDTPTTNRFSKNPNTLPVYQAF
                     PNLDYNPGKGILAYKTSPADYDNWLQYNFDDKSEVAFNFTDALKISDYLSKNGNFDFY
                     SLHQSYSWFLPSFKLKEPTNEESELTANLAICYGAKGMMYFALNGNVTDNGYNSDYGN
                     NGTSFFIPPLPVFSKSLPEDQLNITADLKQTF"
BASE COUNT         1177 a          470 c          590 g         1046 t
ORIGIN      
        1 ttaatattta agtttcaaat aaaaatatga aagctataaa aaaaatattt gatccaaata
       61 aactctcgac acttctgttc tattcgagca tactttcttt tatcatcgca ttcaatttcg
      121 cacataatcc tccgggtggg tggacacagc agtatatgcc gaatctgaat aacagacctc
      181 ttgtggggct ggaatttact gatagtttga tcgggtattc tatattaagt agtcaagcta
      241 ctgatacaag ttttcttatt aaatctacta atggtggtga tgactggaat acaatatttt
      301 ttgaatttag aaaatataat tgtttccagt ttttaaataa ttcaatagga tttattggtg
      361 gtggagattt agaatttggt caatcttatt taattaaaac caataacggt ggtgaaaatt
      421 ggattagatt gaatactccc aatagttctt caatttcaag tatttctgtg ttgagtgaag
      481 actctatttg gatatcagtt gctagcggtt tcgatggcgg gatattcaga acaacaaatg
      541 gagggatgaa ctggacatat cagggtctgc gttcaaatgg tattgaaaga atatatatgt
      601 ataatggaaa tgtaggattt gcatcttctc aaaattatat ggtcagaact acaaactccg
      661 gaattaactg ggatattatt cagggacaga atggatacag agatattagt tttattgaca
      721 gcttgacagg atggaaatca tatggagata ctatgaaatt tactacaaat ggaggtttaa
      781 attggagtag tcagaaatta ccaattggcg gaaaaataga agttagtatt atgtctaact
      841 ttgataacat taataatgac actctttggg gatgtggggg ttgggttcga tatcctaata
      901 atcaatttag aggtattttg tatagaagta caaataaagg attaatttgg gaatatcaaa
      961 ttccagacac ttcaataaac attggaatat atgaaaaaat agattttgta tctaatagta
     1021 tcggttggtc ttatatacag tatcctaatt atataacagg aatacataca acagttggtg
     1081 gagatacatc atttataaca aacataaaat cttcacctat catttcaaat cacgatttta
     1141 aattgttccg gaattatcca aatccgttta atccaaaaac aaaaatctca tacgagttaa
     1201 aacaaaaagc ggaagttaca attaaagtgt ttgatatacg cggaaatgaa attcggactt
     1261 tagtaaatgc aaacatattt gaaggatata atgaagttga atttgacgga acaagatttt
     1321 catccggaat ttatttttac cggatggaag taaagacaag caaagaatat tttacagatt
     1381 ctggaaaaat gattttactt aaataatttg aagaattaaa taataaatag ataaaacgaa
     1441 tatttttaaa actaatataa tacaaatatg aaaaagaaaa ttttactcag ctttgtctta
     1501 atgacaattt catttttaat gtttataagt ttaacaggga tagataatcc tgatgaaaaa
     1561 ttcctcattg tggctatgca cagtggaatt gactcaactt cagcaaatta ttccagaata
     1621 aaagattcac ttggaatgaa cggatggcac aaatataatc aatggtttga acatgatgag
     1681 ataacttcag atatttcttt cttatcaccg cgtgttcaat acagggtaaa tgaaaacaac
     1741 acaaaggatt tacgaactgt atttggaaga agaattactg attataccgg ctggggacaa
     1801 agaagcgact atcaatgtga agtaattccg tatggaaatg attattggtt ttattcatat
     1861 gatacttcag ttattaatac ctggataaat gatattcagg attcaaatgc tactgtaaaa
     1921 tattgtgatg cgatagaatc aagtccgggc agtcatgcca gttatattat aaaaggatta
     1981 aaagcgaacc gcgaacaggc aaaccgtaga tggactccat ggcaatctga tacatatagt
     2041 tcttggtata taaaaccaaa gattagaatt ccaacaggtt tatcacctaa cacattagtt
     2101 tgtacaattg aaatacttga ctgggatggt acgattgtaa gaagtgttgt tctaaaagcc
     2161 aataattttg aaagaagttc tttaagtccc tatcaaggaa attatttaga tgagttttac
     2221 tttgtaccaa atcaaaatgc ttccccaata aaaaccgatt ctacagaact atgccccgga
     2281 tcgaatttga aagattttac aaactgggga gataccaacg caattataaa aacggatttc
     2341 agagtttact ggcatgggga atgtgatatg tggatagatc gtattcgggt tgagaatgaa
     2401 caggcgatac aactatttca agaaggagga gatgctgatt taagaatcag atctgaagta
     2461 tcaagtgctt tattagatta tgacccatcc cgtccaaatc atttttacct tgaggaattt
     2521 gaattcaata caacaccttg tataaaaaag gtcagagaga tcatagaaga agaatcacaa
     2581 ggaaaactta catttattgc taatctaaat ccctatatgt ataattttca tgtaccgaat
     2641 tatgtaagtc atccattcac tgctgatgag ttcaaaaaat attttataga tcctacaact
     2701 ggcggaggtg tcaagatagc tgccgcaact ttttattttc ttgaaggatt tgaagacact
     2761 cctacgacaa acagattttc aaaaaatcca aatacacttc ctgtatatca ggcatttcca
     2821 aatttagatt ataatccggg taaaggtatt cttgcatata agacatctcc tgctgattat
     2881 gataactggc tacaatataa ttttgatgac aaaagtgagg ttgcatttaa ctttactgat
     2941 gcattaaaga tatcggatta cctatccaaa aatggtaatt ttgactttta ttctctacat
     3001 caatcttact catggttttt accttctttt aaattgaagg aacctacaaa tgaagaatct
     3061 gaattaacag caaatctcgc aatatgttat ggagcaaagg gcatgatgta ttttgcattg
     3121 aatggcaatg tgacagataa tggttacaat tctgattatg gaaataatgg aacaagtttt
     3181 tttattccgc cgttgccggt gttttcaaaa agtttgcctg aggatcaatt gaatattact
     3241 gcagatctta agcaaacttt ttgatcaccg gcaacaaacc gaa
//
LOCUS       DAORVQ010000290         3279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_25027_length_3279_cov_4.770161, whole genome shotgun sequence.
ACCESSION   DAORVQ010000290 DAORVQ010000000
VERSION     DAORVQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25027_length_3279_cov_4.770161"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PLX80_14165"
     CDS             complement(<1..174)
                     /locus_tag="PLX80_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY21983.1"
                     /translation="MQIEDIKKIGVIGSGTMGRGIAICSALSEIDTIVYDIKDEIVNS
                     SKSLTEKDLSKSVE"
     gene            complement(238..1548)
                     /locus_tag="PLX80_14170"
     CDS             complement(238..1548)
                     /locus_tag="PLX80_14170"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQY21984.1"
                     /translation="MNLDKFTSYNTALKYLFELERAGIKYDLSNIRKLLKILGNPHKN
                     FKSVHIAGTNGKGSVSSIINSVLIEKGIRTGLYTSPHILDFRERILVNGKFISKKFIL
                     GFVNRMYDEIEKIKPSFYEVTTAMAFEYFSFMKMEISVIETGLGGRLDSTNIITPLIS
                     VITGISIDHIEYLGSSISSITREKGGIIKKKVPVVTGNLKPVSKKILSSIALEKNSPI
                     YNSFKEKNYEIAERTETGFYFTLNKKKERYFFPVIGDYQIENICTAVNTLKILKDTYG
                     IAVDSDILKKGFQNIKINSGYKGRFEIVSEKTSDSPKLITDISHNVQGIKNIENNLKY
                     FKFDKAVIIFGMMNDKNYKDCILELKRLNHHVIFTKPEYSRSAKPEDLYNVIKGNKEK
                     FDHRKNFKEAYELALSMTKKNDLILITGSFFLVSDFLRLLNTLK"
     gene            complement(1570..2055)
                     /gene="ribH"
                     /locus_tag="PLX80_14175"
     CDS             complement(1570..2055)
                     /gene="ribH"
                     /locus_tag="PLX80_14175"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQY21985.1"
                     /translation="MIKEIKADSNGKDLRIGIIISKFNKPITENLLKGALKALESCEV
                     EKKNIEIFYVPGAFEIPIVLESLCSKNAEAKKFDGFITLGCVIKGETAHFEYICDSVS
                     QSINRLSCEYLIPVGFCVLTCYTPLQAYERSLIPPDAENNKGFESALTVIEMINLLKN
                     I"
     gene            complement(2064..2894)
                     /locus_tag="PLX80_14180"
     CDS             complement(2064..2894)
                     /locus_tag="PLX80_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQY21986.1"
                     /translation="MTNYKADLHLHTNYSDGVLSPSKLIDLAKKNDLSVISITDHDNV
                     NGIEEAIEYGNSVGIQVIPGVEISADIDDNEIHILGYFIDHKNPEFLDFLKNSRQMRI
                     ERNEKIVNKLNDLGSKINFDDILKKVGSQTSLGRPHIAMELYDEGFVKTYYEAFFKYI
                     GDDKTAYVRKSNPDAKEVIEMISKCKGLSFIAHPGKYIRDEKLLKLTEHGLDGIEVVH
                     PSHDKESMAYFNKIISEKFLLSSGGSDFHGGIKNDSSSLGKFCISGTEIQNMKRRLFI
                     "
BASE COUNT         1048 a          556 c          441 g         1234 t
ORIGIN      
        1 ttcaactgat tttgaaagat ccttttcggt taaacttttt gatgaattaa ctatttcatc
       61 ttttatatca taaacaattg tatcgatctc tgataatgct gagcatatcg caattcccct
      121 gcccatagta cctgagccaa tcacacctat cttttttatg tcttcgattt gcattttgga
      181 ttattttaaa tattgaattg tatggataat ttttataaat ataaaatttt atatcgacta
      241 ttttaacgta tttaacaatc tcagaaaatc acttactaga aaaaaagaac ctgtaattaa
      301 aatgaggtca tttttttttg tcatacttaa tgcaagttca tatgcctctt taaaattttt
      361 tctgtgatca aatttttctt tgtttccctt tatcacatta tataaatcct cgggtttcgc
      421 agatctgcta tattcaggtt ttgtaaagat cacatgatga ttaagccgtt taagttctaa
      481 tatacaatct ttataatttt tatcattcat cataccgaaa ataattaccg ctttatcaaa
      541 tttgaaatac tttaaattat tttctatatt ttttatacct tgtacattat gagaaatatc
      601 tgtgattagc ttaggactat cagatgtttt ttctgataca atttcaaatc tgcctttata
      661 tcctgaattt atttttatgt tctgaaagcc cttctttaat atatctgaat ctactgctat
      721 tccataagta tctttcaata tttttaatgt gttcacagct gtacaaatat tttcgatctg
      781 ataatcgcct attacaggaa aaaaatatct ttcctttttt ttatttaatg taaaataaaa
      841 tccggtttcc gttcgctctg caatctcata attcttttcc ttaaatgaat tataaattgg
      901 tgaatttttt tcaagggcaa tggaagataa aattttcttt gaaacaggct ttaaatttcc
      961 tgtaactaca ggaaccttct tcttaattat gccacctttt tcacgggtaa tagatgagat
     1021 actgctgcca agatattcaa tgtgatctat ggaaatgcct gtaattactg atatcaaagg
     1081 tgtgataata tttgtagaat ctaatctgcc gccaagtccg gtttcgatca cagaaatttc
     1141 cattttcata aaactgaaat attcaaatgc catcgctgtt gtcacttcat aaaaactcgg
     1201 cttgatcttt tcaatttcat catacatcct gtttacaaaa ccgagtatga attttttaga
     1261 gatgaacttc ccatttacaa gtattctttc tctgaaatca agtatatgcg gagaagtata
     1321 aagacctgtc ctgatccctt tttcgatcag tactgaatta attatggaag agacagatcc
     1381 tttaccatta gtacctgcga tatgaacaga tttaaaattt ttatgaggat tcccgagtat
     1441 tttcaaaagt tttctgatat tactaagatc gtactttata ccggctcgtt ccagttcaaa
     1501 tagatatttt agagctgtat tataacttgt gaatttatcc agattcatta aataaattta
     1561 aaaggcaggt cagatatttt ttagcaagtt tatcatttcg atcacagtaa gtgcggattc
     1621 gaaacctttg ttattttcag catcaggtgg tataagactt ctttcgtatg cctgcagcgg
     1681 tgtataacaa gtcaacacac agaatccaac aggtattaaa tattcacatg ataatctgtt
     1741 aatactttga gaaacactgt cgcagatata ttcaaaatgt gcagtctcac ctttaataac
     1801 acatcccaat gtgataaatc cgtcaaattt ttttgcttca gcatttttac tgcaaagaga
     1861 ctctagcaca ataggaattt caaatgcacc cggtacataa aatatttcaa tatttttctt
     1921 ttcaacttcg caggactcaa gagctttcag agcacctttc aaaagatttt cagtaattgg
     1981 tttattgaat ttactgatga tgatccctat cctcagatct tttccgttgc tgtccgcttt
     2041 tatttctttt atcataattt gatctaaata aacaatcttc ttttcatatt ctgaatctct
     2101 gtcccgctga tacaaaattt tcctaagctt gatgaatcat ttttaatacc gccatgaaaa
     2161 tccgaaccac cgctcgacaa aagaaatttt tctgaaatta ttttattaaa ataagccatt
     2221 gactctttat catgtgaagg atggacaact tcaattccgt caagaccgtg ttctgtaagt
     2281 tttaataatt tttcatccct tatatatttt cccggatgag caatgaaaga caaaccttta
     2341 cattttgaga tcatttcaat tacttctttg gcatccggat ttgatttcct tacataggca
     2401 gttttatcat caccaatata cttaaaaaac gcttcgtaat atgttttgac aaaaccttca
     2461 tcataaagct ccattgcaat atggggacga ccgagtgaag tctgtgaacc gacttttttc
     2521 aatatatcat caaagttgat cttgctgccg agatcattaa gtttatttac gatcttctca
     2581 tttcgctcaa tcctcatttg tctggaattt ttcaaaaaat ctagaaactc agggtttttg
     2641 tgatcaataa aatatcccag aatatgaatc tcattatcat caatatcagc gctgatctct
     2701 actcccggaa tgacctgaat accaacagaa ttgccgtatt caatcgcttc ttcaattcca
     2761 ttgacgttat catgatcggt aatactgatc acagaaagat cattcttctt tgcaagatca
     2821 ataagttttg aaggtgaaag cacaccgtcg gaatagtttg tatgtaaatg aagatcggct
     2881 ttgtaatttg tcaaggctta aaaaagatta tatccaattt aatctttatg aatatttatt
     2941 gaaaggcata ttatcccaat gctgttaaat ttttatagta actaaaataa tttgatataa
     3001 aatctcttaa gaagcttagc agatctgttt tgataagatc aaatatagct tatttcaaag
     3061 caggtaactt aaaattttcg gtatgattat ttagaaattc ttttgatttt atcaaattaa
     3121 tgatgattta aaaaccgttt attatttttt tcaaatcata gatatagtca ggacttaagt
     3181 ttatttcttt tgcagaatca ttttaatttt tttagtttga tgcagattca ataaaaattt
     3241 ttaaaaatta aaaatcagaa taatgaaaaa cttaaaaat
//
LOCUS       DAORVQ010000291         3218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_25904_length_3218_cov_2.794499, whole genome shotgun sequence.
ACCESSION   DAORVQ010000291 DAORVQ010000000
VERSION     DAORVQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25904_length_3218_cov_2.794499"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLX80_14185"
     CDS             <1..353
                     /locus_tag="PLX80_14185"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HQY21987.1"
                     /translation="TSTTFNYSTSGEGPLSFSSLFKKDIDYLNYITTICIKDLSLRLN
                     DKDEDNTDMILDGASADEKNFTNWSVKDDTLTIIFNPYQVAPYVYGMQDVGIPLSELM
                     TYLDSEGPLSYMFR"
     gene            complement(540..1445)
                     /locus_tag="PLX80_14190"
     CDS             complement(540..1445)
                     /locus_tag="PLX80_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY21988.1"
                     /translation="MADSNTAKSNTLKAVIATLTGFFFLASISLLVKLEIAGGASIEW
                     VVFMQYFTCLVIISILASKYKFRDLKTTKIKYHVIRGITGILAFTFFVIAVSEIPLVN
                     ANLLNNTAPLFIPILTIFWLKSKIDGKIWWGILIGFIGIVLILDPTDGNFFELGDMFG
                     IGAGLLLAFAFVALGILTKTESFITILFYYSLIAFTLSLPFAIANWSNPPLLIWIYAV
                     LTGVLFISYLYLLQYAYRYVSVVKLSPLNFSVVIFAGILDWLFYGHVPGFSSLIGIVL
                     VITGGILALTLHQKKNPHIKHHWHF"
     gene            complement(1480..2037)
                     /locus_tag="PLX80_14195"
     CDS             complement(1480..2037)
                     /locus_tag="PLX80_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21989.1"
                     /translation="MSKIIQKTYTLKNGLAINIRNARLIDAEQLIDINLSIIDENLYM
                     LRSSGEAFYTPEGEKEKIIRNNGSEGSLYIVAEADGKVRGYLDFQNGGFRRTRHSGSF
                     SIYISKEYRNIGIGEILINTLIDWAKSGNLIEKITLAVFSTNEKAMKLYNKLGFIEEG
                     RCPKDMKLEDGTYMDSVLMYKFIEV"
     gene            complement(2061..2729)
                     /locus_tag="PLX80_14200"
     CDS             complement(2061..2729)
                     /locus_tag="PLX80_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQY21990.1"
                     /translation="MSDKAVIFDMDGVLIDSEPAYLEMNLKMFEQFGIEMDEENYKAL
                     VGIPSVPMWKFLKEKYELKNEVSEFINLEKKRMLEILDSDIITKPVTGIKELMDDLKS
                     FNFKLSVASSSAKDNINFVLEKLKIKDNFEFIISGEDVVNGKPFPDIFLKVSDFFNIS
                     PEKCFVIEDSVNGIKAANSAGMNSIGFINDGTNTQDLSKADIIINNFDSPGRKKTMDF
                     ILNI"
     gene            complement(2743..>3218)
                     /locus_tag="PLX80_14205"
     CDS             complement(2743..>3218)
                     /locus_tag="PLX80_14205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY21991.1"
                     /translation="RNCKFFEPWIPEYEKNYYTYDYQILKLEYEKKLKEKGIEYRFYI
                     FDKNIPERIIGNVSVSNIIRGVLQSAFLGYSVDEEKNRKGIATEAVKRIIEFTFDELI
                     LHRLEANVIPSNTASIKVLEKLNFVKEGYSENYLKINGRWQDHLRYALINKNYSG"
BASE COUNT         1029 a          599 c          501 g         1089 t
ORIGIN      
        1 caaccagcac aacctttaac tattcaactt caggtgaagg tccactgagc ttttcttctt
       61 tgttcaaaaa agatatagat tatttaaatt acattactac aatttgcatc aaagaccttt
      121 ctttaagact gaatgataaa gatgaagata atactgacat gatactcgac ggagcttccg
      181 ccgatgaaaa gaattttacc aactggtcgg ttaaagatga tactcttacc ataattttta
      241 atccgtatca ggttgcgccg tatgtttacg ggatgcagga tgtcggaatt ccgctaagtg
      301 aattaatgac ttatctcgat tcggaaggac cattgtcgta tatgttcaga tgagataatt
      361 ttccttttcc gatattttgc tttataacca cataggaaca tagggcacat agaaatgttt
      421 tatattctat gttgataaca atcttcaact tttaactttc aacttttaac ttttaacttt
      481 caacttttaa cttttaactt ttaactttta actttcaact tttaactttt aacttttatt
      541 caaaagtgcc agtgatgttt tatgtgagga ttcttttttt gatgaagagt taaggcaagt
      601 attcctccgg ttatgacaag tacaattcca atcaaagatg aaaatcccgg aacgtgacca
      661 taaaaaagcc agtcgagtat tcctgcaaaa atcacaacag aaaaattcag cggagataat
      721 tttactacag aaacatatct gtatgcatat tgaagaaggt acaggtaact tataaaaagc
      781 actccggtca gaacagcata tatccagata agcaatgggg gatttgacca gttggctatg
      841 gcaaaaggca aagagagagt aaaagctata agtgaatagt aaaacagtat cgtaataaaa
      901 gattcagtct tagtgagaat accgagagcg acaaatgcaa acgccagcaa tagcccggca
      961 ccaatgccaa acatatcacc cagttcaaaa aaattaccgt cagttggatc taaaattaaa
     1021 acgataccta taaatccaat aagaattccc caccaaattt taccgtcaat tttgcttttc
     1081 agccagaaga tagtgagtat agggataaat aaaggagcgg tattattaag aaggttagca
     1141 tttacaagcg ggatttcaga tacagctatc acgaaaaaag taaaagccaa tattccggta
     1201 attccccgta tgacatgata cttgatttta gttgttttaa gatcacggaa tttatattta
     1261 gaggctaaga ttgagataat gacaagacat gtaaaatact gcataaatac tacccactcg
     1321 atcgaagctc ctcctgctat ttccagtttc accaaaagac taatggaagc aagaaagaaa
     1381 aaacctgtaa gcgtagcaat gacggctttc aaggtatttg attttgcggt attgctatct
     1441 gccatttata aatatttatt aataaatata actgatttac tatacctcaa taaatttgta
     1501 catcagcaca ctgtccatat aagtaccgtc ttcaagcttc atatctttcg gacatctgcc
     1561 ctcctcaatg aatcccagct tgttatatag tttcatggct ttttcattag tggaaaacac
     1621 ggcgagagtg atcttttcaa ttaagttgcc gctcttcgcc caatctatga gagtatttat
     1681 cagtatctct ccaattccga tgttacggta ttctttgctg atgtagatag aaaatgaacc
     1741 tgaatgccgg gtgcgtctga atcctccgtt ttgaaaatca agataacccc ttacctttcc
     1801 atcagcttca gctacaatgt aaagcgaacc ttcagatccg ttattcctga ttattttttc
     1861 cttttcccct tcaggagtat aaaaagcctc tcctgatgaa cgcagcatgt aaagattttc
     1921 atctatgata ctgagattaa tatctattaa ctgttcagca tctattaatc gggcatttct
     1981 tatattaatg gcaagtccgt tttttaaagt ataagttttt tgaatgatct tactcatctg
     2041 taaattttaa gtaaaaggtt tcagatattt agtatgaagt ccatagtttt ttttcttccg
     2101 gggctgtcaa agttatttat aataatatcg gcttttgaaa gatcctgtgt gtttgttccg
     2161 tcattgataa atccaatact attcattccg gctgaattag cggctttgat accgtttaca
     2221 gaatcttcta ttacaaaaca tttttccgga gagatattaa aaaaatcaga gacttttaaa
     2281 aaaatatccg ggaatggttt gccgtttacg acatcttcac cgcttattat gaattcaaaa
     2341 ttatctttga tctttagttt ttcaagaaca aaatttatat tgtccttagc cgaagatgaa
     2401 gccacgctta atttaaaatt gaatgattta agatcatcca tcagctcttt tattccggtt
     2461 accggttttg taataatatc agaatcgagt atttcaagca ttcgtttttt ctcaaggttt
     2521 atgaattcac tgacttcatt tttaagctca tatttttcct taagaaattt ccacattggc
     2581 acagaaggta taccgacaag agctttataa ttttcttcat ccatctctat tccaaactgt
     2641 tcgaacatct tcagattcat ttcaagataa gccggttctg aatctatcag tactccatcc
     2701 atatcaaaaa taactgcttt atctgacata tcgatcatat tttcacccgg aataattctt
     2761 attaattaat gcataccgca aatgatcctg ccacctgccg tttatcttca ggtaattttc
     2821 tgaatatcct tcttttacaa aatttaattt ttccagaacc tttatagatg ctgtattgga
     2881 gggaattaca tttgcttcaa gtctgtgcag tataagttca tcgaaggtaa actcaatgat
     2941 tcttttaacc gcttctgttg cgataccttt acgatttttt tcctcatcca cactatatcc
     3001 cagaaaagca ctttgaagaa cacctcttat gatattggaa actgaaacat tgccaattat
     3061 cctttccgga atattcttat cgaatatata aaatcgatat tcaatccctt tttcctttag
     3121 ttttttttca tattcaagtt ttaagatctg ataatcatac gtatagtagt ttttttcata
     3181 ttccggaatc cagggttcaa aaaatttaca gttacgtt
//
LOCUS       DAORVQ010000292         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_26568_length_3173_cov_4.755613, whole genome shotgun sequence.
ACCESSION   DAORVQ010000292 DAORVQ010000000
VERSION     DAORVQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26568_length_3173_cov_4.755613"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1344
                     /locus_tag="PLX80_14210"
     CDS             148..1344
                     /locus_tag="PLX80_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY21992.1"
                     /translation="MLILFNKLTQEEIVKFERIVNSSYFNTVENVKKLFYYLKSKHPG
                     ITEKDISQTEISKQIYSDETISTEMIWKLKSDFSKVFEKFLVQNVFENDKAYNDMCLL
                     KELREKGSEKEFKLLLNNLKKQSDKQFIKEDLYYMNKVTINNEEIYFRFTNIQFELHE
                     NLQAKSDNLDYYFLFMKFHTFHEMIMYQYQNGKILNFNKNFFDELTGFVKKNEKSISK
                     DHPDVYVIYLVLMMFVNKDKKNFSVQFKKYLKLNVGKFTNNQLSYFYRYLESFYHLKI
                     NEGHTELRTELFELYKIMFDKDLFVLVNSITDKDFNNVINIVLPLGKFNWAEQYMEKY
                     KSFIVPEMAEESYNLAKAKLYFYKKDFDKIFPHLNLIHYKILIITLIQKFFRQKFYMK
                     LMRWIH"
     gene            1820..3118
                     /locus_tag="PLX80_14215"
     CDS             1820..3118
                     /locus_tag="PLX80_14215"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HQY21993.1"
                     /translation="MKRKNNVGRNSLIVSIAGLAVIEILFYAGAIEGEIWRIVEIGFE
                     AATVGGIADWFAVSALFREIPIPVPFIKKHTNIIVNNRKKLTDGASDLVTSKWLSKES
                     LKEKISGFDFSGFILENLNDPNSRYKENITDLIRDIALRFVSVIDRPDIIEFMKKIFA
                     DQIKDLDLAKPLGNWIKRSFDEGKHEQLWEMLIRNTGKVINSETTRKLLDQEIDKLVQ
                     NYKDQGVVKKMFVSIAEKFKGIDREIIAEQLINVINEFLKEAENNPGHKFRKDLDKVV
                     LDFADGLINSDPSSVKIISDLKENILNNPDVDKLLHNALTNLKKTISVQLENTETPLM
                     KTIIENLDRIIEEINVNKDSQLKINNWIKDNLSEMIDKHHYKIGEHVIDSMNKLDDKE
                     LVEQIEEKVGDDLQFIRLNGAIVGGLAGILIGLLKVMIFS"
BASE COUNT         1224 a          436 c          589 g          924 t
ORIGIN      
        1 aaaactgtct gggaaatatt aaatttgaac ttgatgcaaa ttaaattaat taatgataga
       61 atccaaagga caatttttta aaaacctgaa agttgaatag aacatttaaa ttcaatccgt
      121 tattattcaa aaacaaaatg ccggagaatg ctaatattat ttaataaact tacccaggaa
      181 gagatcgtca agtttgaaag aatagtgaat tcctcttatt tcaatacggt tgaaaacgta
      241 aagaaattat tctactatct taaatcaaaa caccccggga ttacagaaaa agatatttca
      301 caaacagaaa tttcaaaaca aatttattca gatgaaacta tttcaaccga aatgatctgg
      361 aaactcaaat cagatttttc gaaagtgttt gaaaagtttc ttgtacagaa tgtatttgaa
      421 aatgacaaag cgtataatga tatgtgtctg ttaaaagaac tgagagaaaa aggatcggag
      481 aaggagttta aacttttatt aaacaatctg aagaagcaaa gcgataagca atttataaaa
      541 gaagatctat attatatgaa caaagtgaca ataaataatg aagaaattta tttcagattt
      601 acaaatatac aatttgagct gcatgaaaac ttacaggcaa agtctgacaa tcttgactat
      661 tattttctct ttatgaaatt tcatacgttt catgagatga tcatgtacca gtatcaaaac
      721 ggtaagatac tcaatttcaa taagaacttc tttgatgagc tgacaggttt tgtgaaaaaa
      781 aatgaaaaat caatttcgaa agatcatccc gatgtctatg taatatatct ggtcttaatg
      841 atgtttgtga acaaagataa aaaaaacttt tcggttcagt ttaaaaaata tcttaaactg
      901 aatgtgggga agtttacaaa caatcagctc agctactttt acagatatct tgaatctttc
      961 tatcatttaa aaataaatga gggacatact gaattaagaa ccgaattatt tgaattatat
     1021 aaaataatgt ttgataaaga tttgttcgtt ttggtcaatt ccattactga taaagatttc
     1081 aacaacgtta taaatattgt actgcctctc ggaaaattta actgggctga acaatacatg
     1141 gagaaataca aaagcttcat agttccggag atggctgaag agagttacaa tctcgccaaa
     1201 gcaaaattat atttttataa aaaagatttt gataaaattt tcccccactt aaatttaatt
     1261 cattataaga tcctcataat tacattaata caaaaattct tccggcaaaa gttttatatg
     1321 aaactaatgc gctggattca ctgacgagca tactggacag cttacgaaaa tatttaagac
     1381 gtgataaaaa tttaaatgag ccgcagattc aaactttaaa aacatttata aaatttatat
     1441 ctattttatc aaagataaaa agcggaaata acacggatga aatagccgta ctgaaaagaa
     1501 tgatggaaaa tgaaaaaaaa tttatttcga gcaagagttg gttaaaggaa aagatcgaag
     1561 agatagaatt taaaaagatc agaaaaaaat gaattttaag tagaaaaatt aacttcttaa
     1621 ctgaaaagaa ccgttatgaa acaaattaca aaaatgaaat ttaagtctta catcaaaatg
     1681 attcttgccg gtaaaataaa ggtatgaaaa gttaaatata attctgaaag aagttaaact
     1741 taagtaatac ttcaggtgga aatggaatat cggaattgct atatttgtaa atacttattt
     1801 aatgaaataa tttcaaatat tgaaaagaaa aaataatgtc ggaaggaatt ctttgatcgt
     1861 ttcgattgcc ggattggcgg tgatcgaaat tcttttttat gcaggagcga tagaagggga
     1921 aatatggagg atcgttgaaa ttggttttga agctgcgacg gtgggtggaa ttgcggactg
     1981 gtttgccgta agcgcattgt tcagggagat cccgattccc gtgcctttca taaaaaaaca
     2041 taccaatata atagtcaata atagaaagaa acttactgac ggtgcgtctg atctggttac
     2101 aagtaaatgg ctttcgaaag aaagtctgaa ggaaaagatc agcggttttg atttttcggg
     2161 atttattctg gaaaatctaa atgatccgaa ttccagatac aaagaaaata ttactgactt
     2221 aatccgtgat attgctctga ggtttgtttc cgtaattgac agaccggata ttattgagtt
     2281 tatgaaaaaa atatttgctg atcagatcaa agatctggat cttgcaaagc cgctggggaa
     2341 ctggatcaaa agatctttcg atgaaggaaa gcatgaacag ctctgggaaa tgctgattag
     2401 aaatacggga aaggtcatta acagtgagac tacacggaaa ctgctggatc aggagattga
     2461 taaacttgtt cagaattata aagatcaggg agttgtcaaa aagatgtttg tatcaattgc
     2521 cgaaaaattc aaagggatag acagagaaat tattgctgag cagttaataa atgttatcaa
     2581 tgaatttctc aaagaagcgg aaaataatcc cggacataaa ttcaggaaag atcttgacaa
     2641 ggtggtactt gattttgctg acggactgat caatagtgat ccttcatctg tgaagatcat
     2701 ttccgatctt aaagaaaata ttctgaataa tcctgatgtg gataaacttc tgcacaatgc
     2761 ccttaccaat ctgaaaaaaa ctatttcagt acagcttgaa aatactgaaa cgcctctcat
     2821 gaaaaccatc attgaaaatc ttgacaggat cattgaagag atcaacgtga ataaggattc
     2881 acaattaaag atcaataact ggataaagga taatctttcg gagatgatag acaagcatca
     2941 ttacaagatc ggtgaacatg taattgacag catgaataaa ctggatgata aagaacttgt
     3001 agagcagatt gaggaaaagg tcggtgatga tctgcagttc atacggctga acggcgcaat
     3061 agtaggcggt cttgcgggga tattgatcgg gttgctgaag gtgatgatat tcagttagaa
     3121 attgggaatt ctgaatttga atcgccgcga tcagcaaacc cctcccagcc tcc
//
LOCUS       DAORVQ010000293         3171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_26599_length_3171_cov_4.309050, whole genome shotgun sequence.
ACCESSION   DAORVQ010000293 DAORVQ010000000
VERSION     DAORVQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26599_length_3171_cov_4.309050"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..2178
                     /gene="dnaG"
                     /locus_tag="PLX80_14220"
     CDS             334..2178
                     /gene="dnaG"
                     /locus_tag="PLX80_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQY21994.1"
                     /translation="MLIPPEKISEIAQANDIIDVISGYIQVKKRGKSFLALCPFHADK
                     TPSLHISQQKQVYHCFSCKAGGNVFSFIVNYEHVTFIDAVKKLAERAGITLAYESQSP
                     DKSSEISKLYEINKAAAVYFRDNIRKLKSSEKELVDEYLKSRDLDKKTIDKFGIGYSY
                     KEWDGLLHHFEEDEVFSKDEIESSGLILKKEGVKTSYYDRFRGRLMFPIFNDYDKIVG
                     FGGRKLYEDDPGGKYINSPETKVYSKSRILYGLNFAKEKIRYYDSVILVEGYMDLIAL
                     HKSGFENVVASSGTALTEDQTRLLARYTKNVYILFDSDTAGIKAAKRGIEILLENGFD
                     ISIISLPFGEDPDSFIKSNGKKEFEKRISEKQSFINFIGDFYYNENKLNTVEEKTVFV
                     KEMISYISKIPDNIKRPLFIKEISSRYKLSENLLTDEMNKILTAYRKAPFQGSSVVLP
                     VKNAVKSESNGNQTDSFEFELIELFIHGNAEAIEYLESNIHTEYIKNKKILRITEVIL
                     DALINHGLLDIAAVINELDDEEDKQLLTRVGNREYEASIISKPGNDSLLNHTANPVYT
                     IKNAKDIIRRFRIRELETKRMEIRSNPDKLIEIFEITKEINELTNKVK"
     gene            2224..2685
                     /locus_tag="PLX80_14225"
     CDS             2224..2685
                     /locus_tag="PLX80_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009589498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQY21995.1"
                     /translation="MYTIEPIGYVSNSRTTVTDDNWGSIISKIKISHKLSEKCLKGID
                     QYSHLEIIFYFHLADPSKINISSSHPRENPGFPEVGIFAQRKKLRPNLLGSTIVKLLS
                     FEKDTLEVSGLDAVDGTPVIDIKPVLEEFMPAEKVIQPEWTHILMKEYWKK"
     gene            2757..>3171
                     /gene="nusB"
                     /locus_tag="PLX80_14230"
     CDS             2757..>3171
                     /gene="nusB"
                     /locus_tag="PLX80_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQY21996.1"
                     /translation="MPQLKRREVRIKVMQVLYAHEISKEPISKIKKDLMPEVVGEDNV
                     TFSDSLIKHVLENEKLLDEYILGKVLNWELERMALVDRIVMRMGISELLFFPDIPPKV
                     SINEAIDISKDFCTRSSGRFVNGVLDAIHDDLKRDN"
BASE COUNT         1179 a          445 c          584 g          963 t
ORIGIN      
        1 ctcgtccgaa agtgtctcaa aacataattt taattaatac cacaaagaca caaagacaca
       61 aagcattctt tttttaaagt ttttttcttt gagcctttgt gacttaatgg taaaaatagt
      121 gttttgagac agcctcttcc aaagggggat cgttcaaaaa aaacagtaga aaatactaat
      181 atctaagttt actgataaac gttttggacg gatgtggatt tttatacttt accaattttt
      241 tataagagtt aatttttaaa tttgtaattt tcagcagtta aaatattaaa aaaatacata
      301 tgacttttat tgtgatatta tttaatttgc agtatgctaa ttccacccga aaaaatttca
      361 gagattgctc aggctaatga cataatagac gtgatctcag gatatataca ggtaaaaaaa
      421 cgcggtaaat cctttcttgc attgtgtccg tttcatgctg acaaaacccc gtcccttcat
      481 atatctcagc agaagcaggt ctatcattgc ttttcctgta aggcaggcgg gaatgtattt
      541 tcttttattg taaattacga acacgttact tttatagacg ccgtaaaaaa acttgctgaa
      601 agagcaggaa taactctggc gtatgaatct cagagtccgg acaaaagcag tgagatatct
      661 aagctttatg agatcaataa agcagcggct gtttatttca gagataatat cagaaaatta
      721 aaaagcagtg aaaaagagct tgtcgatgaa tacctgaaat ccagagacct cgataaaaag
      781 acaattgata aatttgggat cgggtattca tataaggaat gggacggact gcttcatcat
      841 tttgaggagg atgaagtttt ttcaaaagat gaaattgaaa gctccggtct tatcttaaaa
      901 aaagaaggtg tgaaaacttc ttattatgac cggttcaggg gaaggctgat gtttccgatt
      961 tttaatgact atgataaaat agtcggattt ggtggaagga aattatacga agatgatccc
     1021 ggcggtaagt atataaattc acctgagaca aaagtttaca gtaagagcag aatactttac
     1081 ggattaaatt ttgcaaaaga aaaaataaga tattatgact ccgtaatatt ggtagaaggt
     1141 tatatggatc tgatagcact gcataaaagt ggttttgaga atgtcgttgc atcgagcgga
     1201 acagctttga ccgaagatca gaccagactt ctggcaagat atacaaaaaa tgtttatata
     1261 ctttttgatt ctgatacggc cggtatcaaa gctgcaaaaa gaggtattga gattttactg
     1321 gaaaacggat ttgacatcag tattatttca ttgccatttg gagaagaccc tgacagtttt
     1381 ataaagagta atggtaaaaa agaatttgaa aaaaggatat ccgaaaaaca atcatttata
     1441 aatttcattg gtgattttta ttataatgaa aataagctta atacagtcga agagaaaacg
     1501 gtttttgtaa aggagatgat cagttatatt tcaaagattc ctgataacat taaaagacca
     1561 ctttttataa aagaaatttc ttccaggtac aaactcagtg aaaatctgtt aacagatgaa
     1621 atgaataaaa ttttaactgc atacagaaaa gctccatttc agggatcatc agtagtctta
     1681 cccgtaaaga atgcagtaaa atctgaaagt aatggaaatc aaacagacag ctttgaattt
     1741 gaattgatcg aattattcat ccatggaaat gctgaagcta tagaatatct tgaaagtaac
     1801 attcatactg agtatataaa aaataaaaaa atattaagaa ttaccgaggt gatacttgat
     1861 gctcttatca atcacggatt attggatatt gctgctgtaa ttaatgaact tgatgatgaa
     1921 gaagataaac aactcttgac ccgggtagga aacagagagt atgaagcaag tataatttca
     1981 aaacccggaa acgacagcct tttaaatcat actgccaatc cggtttatac tattaaaaat
     2041 gctaaagata ttataagaag gtttcgcata agagagcttg agacaaaaag aatggagatc
     2101 cggtcaaacc ccgataagtt aattgaaata tttgagatca caaaagaaat aaatgaactg
     2161 acgaataaag tcaaataatt aatcagtaac aggttcattt ttttaaaata ttttaccctg
     2221 aaaatgtaca caatagaacc aatcggatat gtatccaaca gcagaacaac ggttacagat
     2281 gataactggg gaagtataat ttccaaaata aagatcagtc ataaattatc tgaaaaatgt
     2341 cttaaaggta ttgatcaata ttcccatctg gaaataattt tttattttca tcttgcagat
     2401 ccttcaaaga ttaatatctc ttcatcgcat ccaagagaaa accctggttt tcctgaagtc
     2461 ggaatttttg ctcaaagaaa aaaattacgt cctaatttgc ttggttcgac aatagtaaaa
     2521 ctattaagct ttgagaaaga tactttggaa gtttcagggc ttgatgcagt agatggaact
     2581 cctgttatag atattaaacc tgtattagaa gaatttatgc cggctgaaaa agtcattcag
     2641 cctgaatgga cacatatttt aatgaaagaa tactggaaaa aataatttga aaatatagaa
     2701 atttgtaata tatttttaag atattaatga ttagttttga actataaaat ttattgatgc
     2761 cacaactaaa aagaagagaa gtaagaatta aagtaatgca ggttctttac gcacatgaaa
     2821 tatcaaaaga gccaataagt aaaataaaaa aagatcttat gcctgaagtt gtcggagaag
     2881 ataatgttac gttttccgac tctttgatca aacacgttct tgaaaatgaa aaactgcttg
     2941 atgaatatat cttgggcaaa gtcctgaact gggagcttga acgtatggca ttagttgaca
     3001 ggatagttat gagaatggga atttctgaac ttctgttctt tcctgatatt cctcctaagg
     3061 tttccattaa tgaagcaatt gatatttcaa aagatttttg tactcgaagc agcggaagat
     3121 ttgtgaatgg agttttggat gcaattcatg atgatctgaa aagagataat a
//
LOCUS       DAORVQ010000294         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_27202_length_3132_cov_5.714332, whole genome shotgun sequence.
ACCESSION   DAORVQ010000294 DAORVQ010000000
VERSION     DAORVQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27202_length_3132_cov_5.714332"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLX80_14235"
     CDS             complement(<1..181)
                     /locus_tag="PLX80_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQY21997.1"
                     /translation="MDIFHLFSVLIVLSAGFAYINFRFLKLPNPIGLMLVSLIFSLII
                     ILIGQIYPPLRDLLTS"
     gene            complement(192..1097)
                     /locus_tag="PLX80_14240"
     CDS             complement(192..1097)
                     /locus_tag="PLX80_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY21998.1"
                     /translation="MESEIKNFLFNPTVGKIVTIFIGTALIYLLMKFIKGKFLSKIKE
                     GDSRYRAKKFANVLGYLFIVLLITIVFSDKLGGLTVALGVAGAGIAFALQEVIGSFAG
                     WLAIMFGGFYKTGDRVQLGGIKGDVIDIGVLRTTLMEIGQWVDGDLYNGRIVLIANSF
                     VFKEPVFNYSGDFPFLWDEIKIPIQFGSNYDRAREILENAAALRADEISLRSIEKWKA
                     MQQNYRLEAAQTDPMVSVVVNDNWVEYTLRYIVDYKKRRDTKSKLFTKILNDVEAANG
                     EVKFASATFQLVEAPDFNVNMKTKS"
     gene            complement(1105..2634)
                     /locus_tag="PLX80_14245"
     CDS             complement(1105..2634)
                     /locus_tag="PLX80_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HQY21999.1"
                     /translation="MSQQPENIELEFLKTEDYSELKEAMIESYVTMPASIWSEEQIQN
                     LINKFPEGQIVIKVDGKIAGCALSIIVDYSNFDKKHTYRDVTGNFTFSTHNNKGDVLY
                     GIDVFVKPEFRGLRIGRRMYDYRKSLCEKMNLKEIMFGGRIPNYHKYKDEFTPREYIE
                     KVKSKEIHDPVLNFQLSNDFHPVLVLKGYLEGDKASDEYAVLLEWENIYYEKVEIKAK
                     EIKKEIRIGLIQWQMRPYRNVDELMLQSEYFVDALSGYKSDFCLFPEFFNAPLMAEFN
                     DLGEAEAIRKLAEFTPKIIEEFSKLSISYNINIIAGSMPEIVGDVLYNAGYLCKRDGS
                     VERFEKIHVTPDEAKVWGLAGGKNIRTFDTDCGKIGILICYDVEFPELSRILADEGMN
                     ILFVPFLTDTQNAYSRVRVCAQARAVENECFVAIAGSVGNLPKVHNMDIQYAQSAVFT
                     PCDFPFPINGVKTEATPNTEMILISDVNIDLLTELHNKGSVKNLKDRRKDLYNINKLI
                     S"
     gene            complement(2835..>3132)
                     /locus_tag="PLX80_14250"
     CDS             complement(2835..>3132)
                     /locus_tag="PLX80_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQY22000.1"
                     /translation="IKHCAMGILYSLQHLGYTIPPQADAGWIGEAGPGPSYLDPGSGG
                     PENDFTNRNTTFMTWNLMHLARILKDNKGIPAHGNQRSEWDAGCRSDFPNPDYR"
BASE COUNT          913 a          647 c          520 g         1052 t
ORIGIN      
        1 tggaagtaag cagatctctc agaggcggat atatctgacc gatcagaata atgatcagtg
       61 aaaatattag tgataccagc atcagcccga tcggattcgg tagttttaaa aatcgaaaat
      121 ttatatatgc gaatccggct gagaggacga taagtacaga aaataaatga aaaatatcca
      181 acccgtaata tttaagattt agttttcata tttacgttaa aatccggagc ttcgaccagc
      241 tggaaagtgg cagatgcaaa ttttacttca ccgtttgcgg cttcgacatc attaaggatc
      301 ttagtaaaga gcttgctttt tgtatcccgg cgttttttgt aatccacaat ataacgcaga
      361 gtatattcca cccagttatc atttacaacg actgaaacca tcggatcagt ctgtgctgct
      421 tcgaggcggt aattttgctg catagctttc cacttttcaa ttgacctcag tgatatttca
      481 tcagctctca gtgcagctgc attctcaaga atttccctgg ccctgtcata attactgcca
      541 aactgtatgg gtatctttat ttcatcccag agaaaaggaa agtcacctga ataattgaat
      601 acaggttctt taaaaacaaa actgtttgcg atcagcacaa tccttccgtt ataaagatct
      661 ccgtccaccc actgacctat ctccatcaga gtagttctca aaacaccaat atctataacg
      721 tcgcctttga taccgccgag ctgaacgcgg tcgcctgttt tgtaaaatcc gccaaacata
      781 attgcaagcc atcctgcaaa agacccgatc acttcctgta gtgcaaacgc aatgcctgcg
      841 ccggctacac caagcgctac agtcagtccg cccagtttgt cactgaatac aattgttatt
      901 aataatacaa taaataaata tcctaataca ttggcgaatt ttttcgcacg gtatcggctg
      961 tcaccttctt ttatttttga taaaaatttt ccttttataa acttcatcaa taaataaata
     1021 agagcggtcc cgataaatat cgttacgatc tttcctactg tcggattaaa aagaaaattt
     1081 ttaatttcgg attccattat ttttttaaga tattaattta ttaatattgt aaagatcctt
     1141 tctcctgtct tttagatttt ttacactgcc tttgttatgc agctctgtaa gaagatcaat
     1201 attcacatct gagatcagaa tcatttcagt atttggagtt gcttctgttt taacaccgtt
     1261 tatgggaaat ggaaaatcac atggagtaaa aactgcagac tgcgcatatt gaatatccat
     1321 attatgtact ttaggaagat tgccgacact tcctgcaatt gctacaaaac attcattttc
     1381 aacagctctt gcctgagcac agactctgac tctcgaatat gcgttttgtg tgtcagtcag
     1441 gaatggaacg aataatatat tcattccttc atctgcaagt attctgctga gttcagggaa
     1501 ttccacatca taacatatca ggattccgat cttaccgcag tcagtatcaa atgttcgtat
     1561 atttttaccg ccggccagtc cccatacttt tgcctcgtcc ggagttacat gaattttctc
     1621 aaatcgttcg acagacccgt ctcttttaca taagtatccg gcattataaa gtacatcccc
     1681 gacgatctca ggcatgctgc cggcgattat gttaatgtta tatgaaattg aaagttttga
     1741 aaattcttca attatcttag gtgtgaattc tgcaagtttt cttatggctt cagcttctcc
     1801 aagatcatta aattctgcca tcagcggagc attaaaaaat tcaggaaaaa gacagaagtc
     1861 gcttttgtaa ccactcagtg catctacaaa atattcagac tgcagcatca gctcatctac
     1921 attccgataa ggtctcatct gccattgtat aagtccgatc cggatctctt tttttatttc
     1981 ctttgctttt atttcaactt tctcataata aatattttcc cactccagta aaactgcata
     2041 ttcatctgat gctttatccc cttcaagata acccttcagt acaagaaccg gatgaaaatc
     2101 atttgaaagc tggaaattca atacagggtc atggatctct ttggatttta ctttttcaat
     2161 gtattcacgc ggggtaaatt catctttgta tttatgataa ttcggaattc tccctccgaa
     2221 catgatctcc ttcaaattca ttttttcaca taatgacttt ctgtaatcat acattcttct
     2281 gccaattctc aaaccacgga attcaggttt tacaaagaca tcgattccgt ataagacatc
     2341 ccctttatta ttatgagtac tgaaagtaaa gtttcctgtg acatctctgt aagtatgttt
     2401 tttgtcaaag ttactgtaat ctacaattat ggataatgcg catccggcaa tttttccatc
     2461 tacttttatt acgatctgac cttcaggaaa tttattgata aggttctgaa tttgttcttc
     2521 tgaccagatt gaagcaggca ttgtaacata agattcgatc attgcctctt ttaattctga
     2581 gtaatcttct gtttttaaaa actccagttc aatattttca ggctgttgtg acatatataa
     2641 tattataaat taattaatca gattccacac gggatatatt ataaagataa gtaattaaac
     2701 agtattaaag caaatttata aaactgttaa ttggatcacg ctttttggca gcttcaattt
     2761 ccttttcatt tgattcgatt tcttcacagt gaatttcctt aatatacttt cataatgcag
     2821 ttttaaaaat attcttatct gtaatcggga tttggaaaat ccgatcggca tcctgcatcc
     2881 cattcggatc tctgatttcc atgtgccgga attcctttat tatcttttaa tattcttgca
     2941 aggtgcatca ggttccatgt cataaaagtt gtattacgat ttgtgaaatc attttcgggt
     3001 ccgcctgagc cggggtcaag atatgaggga cctggtcctg cttcaccgat ccagcccgcg
     3061 tctgcctgtg gaggaattgt gtatccgaga tgctgtagtg aataaagaat tcccatggcg
     3121 cagtgtttga tc
//
LOCUS       DAORVQ010000295         3126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_27313_length_3126_cov_5.202866, whole genome shotgun sequence.
ACCESSION   DAORVQ010000295 DAORVQ010000000
VERSION     DAORVQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27313_length_3126_cov_5.202866"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1205
                     /locus_tag="PLX80_14255"
     CDS             138..1205
                     /locus_tag="PLX80_14255"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HQY22001.1"
                     /translation="MKKIYTLFFAFLITLSFNSEKSNAQNNVLLEYFTGAWCQFCPCG
                     HSNIEGILANYPNTVVLGYHGGGPDPWAAYSGTMISRFPITGYPQGVIGRRSGVVGRD
                     AYNNEVVLQTLLVQPGVSIEVINKSYDANTRTVNANVKITALMDLTGDYFINYVLTES
                     NMIYPQTGNGGCPGNTNYVHKHVVKSFINGELGELLNSGTMTTGTEIIRNLNYVVPVS
                     PQIEVPENCDLNIFVYKSGASISTTNHVQQAMRTPLTGATGITNNNSAAPKNYTLTQN
                     YPNPFNPTTNFSFSIPTNENVSLKIYDILGNVVDTYVDGFLNAGTYSVVFDGNNLSSG
                     IYFYTLNTGSFSETKRMVLTK"
     gene            1323..2852
                     /locus_tag="PLX80_14260"
     CDS             1323..2852
                     /locus_tag="PLX80_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQY22002.1"
                     /translation="MKTFWIIFISLMFLQSSDKLSAQGIYHNWSTVGGNSNRTGYNDG
                     FFGGAGGFLHIYSSPNTIWGMQIFTNNNRIATTRYVSLNPLKAIVAAYNSFSFNTSWT
                     YGTETGVFVVMGFNDDKVYVRDFRQNGNDSIFALNADDGSVVWRSDFTVERGIIWTAV
                     FADNGDLILPGSGAKRIMRINHMNGDTVWTNSRIIPNTGAENMCVNGNTLYAWQGGLT
                     TPKTIIAIDINTGQIKYSSNTLPGDGDQEIPMTVSESGVVYCIRDGGLMYALKDNGNG
                     FTELWNKIVPHPVGTYSQIGINRDSSVYIPFGKKVYRLDHTNGNAIDSSAELISSGTM
                     NPRFAFDGSRNVYIGNGASDPSQGKYFFLEANLQGLIKEIPMPYNYYSGPAEGGELSN
                     PKILICGAGNEIRSYEYFLQGISGTESQSPDQFTLHQNFPNPFNPVTIIKYQCIMYNE
                     VSLKVFDVLGNEIVTLVNEKQNAGSYSVTFDGSSLSSGVYFYRLDAGDFSETKRMIIL
                     K"
BASE COUNT         1082 a          478 c          588 g          978 t
ORIGIN      
        1 ttggatcaga tttttatttt ttattcaaaa gttttgtcgt gtacagagct tgtaaataaa
       61 tcttaaaaaa aacattgcaa gaaaaaattt aatcatatat tttagttaaa caaaataaat
      121 caaacggggg tatccaaatg aaaaaaattt atacactttt ttttgctttt ctcattacac
      181 tttcttttaa cagcgaaaaa tcaaacgctc aaaacaatgt tttattagaa tacttcaccg
      241 gagcatggtg tcaattttgt ccatgcggac attctaatat tgaaggaatt ttagctaatt
      301 atccaaatac agttgttctt ggctatcacg gcggaggtcc tgatccatgg gctgcttact
      361 ccggtacaat gatttcaaga tttccaatta cgggttatcc gcaaggagta ataggaagga
      421 gaagcggagt tgttggcaga gatgcttata ataatgaagt agttcttcag actttacttg
      481 ttcagccggg agtatcaata gaagtaataa acaaaagcta tgatgcaaac acaagaactg
      541 taaatgcaaa tgtcaaaata actgcactca tggatcttac aggtgattac tttattaatt
      601 atgtattgac tgaaagtaat atgatctatc cacaaaccgg aaatgggggc tgccccggca
      661 atacgaatta tgttcataaa catgtagtga aaagttttat aaatggtgag ttaggtgagc
      721 ttcttaattc aggaacaatg actacaggaa cagagatcat cagaaatctg aattatgtag
      781 tacctgtttc acctcagata gaagtacctg aaaattgcga tctaaatatc tttgtatata
      841 aatcaggtgc aagtatctca actacaaatc atgtacagca ggcaatgaga actccactta
      901 ccggagctac aggcattaca aataataatt cagccgcacc aaaaaattat acactgactc
      961 aaaattatcc aaatccattt aatccgacta ctaattttag tttctcaatt cctacaaatg
     1021 agaatgtaag tcttaagatc tatgacattc ttggaaatgt agtggataca tacgttgacg
     1081 gatttttaaa tgcaggcaca tattcagttg tttttgacgg aaataatctt tccagcggta
     1141 tttatttcta tacacttaat actggaagtt tttcagaaac aaaaagaatg gttctgacaa
     1201 aataaaacag taacaaaaat gcaacactat ctttaaattt atttaatcac tacttgcaaa
     1261 gcgggtctca cgatccgctt ttttatttcg gtataaattt taatttaaaa aatttattaa
     1321 tcatgaaaac attttggatc atttttatca gcttaatgtt tcttcaaagt tcagacaaat
     1381 tatctgcaca gggtatttat cataactgga gcacagttgg tggcaacagc aaccgtacag
     1441 gatataacga tggatttttt ggaggagcag gtggattttt gcatatttac agttcaccga
     1501 atactatctg gggtatgcag atatttacaa ataataaccg gattgcaact acaagatatg
     1561 tatcactgaa tcctttaaaa gcgatagtag ctgcttacaa tagtttttca tttaatacca
     1621 gctggactta cgggacggag acaggtgtat ttgttgtaat gggatttaat gatgataaag
     1681 tttatgtaag agatttcaga caaaatggaa atgattctat atttgcattg aatgctgatg
     1741 acggatcggt tgtatggaga tcagatttta ctgttgagag agggataata tggacagcag
     1801 tatttgctga caacggagat cttattttac cgggctcagg tgcaaagaga attatgagaa
     1861 tcaatcatat gaacggagac actgtatgga caaacagcag aataattcca aacaccggcg
     1921 cagaaaatat gtgtgtcaac ggaaatactt tatatgcctg gcagggaggt ctcaccactc
     1981 caaaaacaat tattgcaatt gatattaata caggtcaaat aaaatattca tcaaacactt
     2041 taccaggtga cggggatcag gaaattccga tgacagtttc tgaatccgga gtagtttatt
     2101 gtatcaggga cggtggtctt atgtatgcat taaaggataa cggtaatgga tttactgagc
     2161 tttggaataa gatcgttccg catcctgtcg gcacttattc acagattggg atcaacagag
     2221 acagttcagt ctatattcct tttggaaaaa aagtttacag acttgatcat acaaatggaa
     2281 atgcaattga ctcttcggca gaattgattt caagtggtac tatgaatcca agatttgctt
     2341 ttgatggatc gagaaatgta tatataggta atggtgcttc agatccttcg cagggaaagt
     2401 atttttttct tgaagctaat ttacagggtt tgataaaaga aattccaatg ccatataatt
     2461 attacagtgg acctgcggaa ggcggtgagc ttagtaatcc aaaaatttta atctgcggtg
     2521 caggaaatga gatcagatcg tatgaatatt ttcttcaggg tatttcagga actgaatctc
     2581 aatcacctga tcaatttact ttgcatcaga attttccaaa tccgttcaac ccagtgacaa
     2641 ttataaaata tcaatgtata atgtacaatg aagtttcatt aaaagtattt gatgttctgg
     2701 ggaatgaaat agtgactctt gtaaatgaaa agcaaaacgc aggcagctat tctgttacat
     2761 ttgacggaag tagtttatcc agcggagttt atttttatag attagatgca ggagattttt
     2821 ctgagacaaa gagaatgata atacttaaat aattacaaat tacaaattac aaattacaaa
     2881 ttgcaaattg caaattgagc ctttttgtta atttacaaac ggcgaaattt taaattaaaa
     2941 atattccagt ggataaatat gaagtctaaa atatgggtgg aaataatttt tagtggtttc
     3001 aaagatcatc ctgatgatat ttcaaattta ctagggattt ctcctagtaa aaagagaaac
     3061 ttatagatct ccaaagggga aagaattaaa ataaattcat ttccgccgtc gatgttggtg
     3121 ttccaa
//
LOCUS       DAORVQ010000296         3124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_27341_length_3124_cov_6.391007, whole genome shotgun sequence.
ACCESSION   DAORVQ010000296 DAORVQ010000000
VERSION     DAORVQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27341_length_3124_cov_6.391007"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..842
                     /locus_tag="PLX80_14265"
     CDS             225..842
                     /locus_tag="PLX80_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22003.1"
                     /translation="MVKYIDSVIGRLESLKSGMEINQAVWNGLSETPGTVQSKIDELL
                     AKEKQMNDAQIEWKVRISESHSLSDMAEKYADKIESLAVGLHSETPEKLGEYEVSQRK
                     KGSVIPKPTRIIYPVLEDDTDGIGFIVSTQKDPDASNYQWFRGVSADPKNVNNVPDMQ
                     PYKVSTKMSFVDDDVEKGVRYFYKVQAFNTHGTGAMSEAVSRIQQ"
     gene            complement(1092..3032)
                     /locus_tag="PLX80_14270"
     CDS             complement(1092..3032)
                     /locus_tag="PLX80_14270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQY22004.1"
                     /translation="MKKKNKIIRNIIFIFILFAINFTSGSLYAQTYFGDYVEVPVYED
                     TIIPNVTNKFDYWDLGQVNTTNNRLDIVVGVSQKGINSGPGNSIIGNWYKNENNDNFN
                     NEVETSRFINLGNYSNPITGLIFAKLSSSPSVKKEVIVSRANGDLEIYSNSLGSILFR
                     IGFKANGKVAAVGNFTTGDNLEDVAVLMDDTVKIYKNLGNGYLDSIPIYRLPMHASAV
                     TLAQISSYAEPYATVNNTTSDKDEIILIQGDSIRVYLNNNNNGTSLSTIIYSGFGFSV
                     KRDFKIADFNNDGYNDLVLVEQDNGINIYKNNSGTLNTTPSYSNTGSFSPFTVAVADF
                     DKNGWNDIVVNLADRINLFLNKKSDSLFQQTVSEFTYYDFPGRLSCVPLKTIVADVYN
                     KGGLAVLYSNFGDVQDLTENDPPNEIEQLVRINASDTDAVPAPAYLFEDFVQQDTIYH
                     PKLLLFNRGDRDFLRYRIYKRNYTSYNYYLIDSTSGDSYTDTTEDLLYSNLDPSDPPP
                     DNLFYYAVAVDNSYKVSITSDTIGYPAYVCPECGGEIGPRGVLAGNEISSTPLNYSIS
                     NYPNPFNPSTKIYYTLPAEGVVKITVYNSLGQKIKEIVNEFKQAGTYKSEFNGSSLSS
                     GIYYYRIESGSFVQTKKMMLIR"
BASE COUNT         1010 a          550 c          565 g          999 t
ORIGIN      
        1 cgctgtagaa gaatcacatg atgtgttatg tatattaccg gacgcggtaa aatattctga
       61 ttatgcgtta tagaaaaata ataatgcgcc ggaaaaatat attgatgcat gtttcgaacc
      121 ggaacaggga tgcctggagt aaggtgcggt aacttatgaa gagtaccttg aaccgtcaaa
      181 aaataatcaa aaattaaata ccgcatttta aaggagacca taaaatggtc aaatatatag
      241 actccgtgat cggcagactt gagtcgctga aatccggaat ggaaatcaat caggcagtat
      301 ggaatggatt gtctgaaacg cccgggactg ttcagtcgaa gatcgacgaa ctcctggcaa
      361 aagagaaaca aatgaatgac gcgcagatcg aatggaaagt gcgtatatcc gaatcgcatt
      421 cgctgagtga catggcagaa aaatatgctg ataagataga atcgcttgca gtcggtcttc
      481 atagtgaaac tccggaaaag ctcggtgaat atgaagtctc acaacgaaaa aaaggttcgg
      541 ttattccaaa gccgaccaga ataatatatc ctgtacttga agatgatacg gacggaatcg
      601 ggtttatcgt gtccacacag aaagacccgg acgcttcaaa ctatcaatgg ttcaggggcg
      661 taagcgcgga tccgaagaat gtaaacaacg tacccgatat gcagccctat aaagtatcga
      721 caaaaatgtc atttgttgat gatgacgttg aaaaaggagt cagatacttt tataaagtgc
      781 aggcattcaa tacgcatggc acaggtgcta tgagcgaggc agtgagcagg atacagcagt
      841 aagattcatt gccggacaat acattcaata acgttccagt tacagcggaa gcaattatcg
      901 ggctgtatgt gagatgagtt ttatgagcag gtagatttat tagttctata aactgcggtt
      961 aatttatttg accgcagttt ttttatattg tgattgtgga aaagcctcgg caaatgatat
     1021 atcaattttg tttataaaaa agccggaacc ataagctccg gctgaattta gattttatta
     1081 aaatttttat tttacctgat cagcatcatt ttcttcgtct gtacaaaact tcccgattcg
     1141 attctgtaat aataaattcc gcttgataaa ctgctgccgt taaattccga tttataagta
     1201 ccggcttgtt tgaactcatt tacaatttcc tttatcttct gaccgagaga attgtaaaca
     1261 gtaattttta ctaccccttc agccggtaat gtgtaatata tttttgttga tggattaaac
     1321 ggattgggat agttactaat ggaatagttt aaaggtgttg atgaaatttc attgcctgcc
     1381 aatacacctc tcggtccgat ctctcctccg cattccggac atacataagc tggatatccg
     1441 atagtgtcag atgtaataga tactttatac gaattgtcca ctgcgactgc ataataaaaa
     1501 agattatcag gcggcggatc agacggatct aaatttgaat aaagcagatc ttctgtcgtg
     1561 tcagtataac tgtctccgga tgtactgtca attaaataat agttataaga tgtataattt
     1621 cttttataaa tcctgtatct caggaaatcc ctgtcacctc tgttaaaaag cagaagcttc
     1681 ggatgataga tcgtatcctg ctgcacaaaa tcttcaaaaa gatatgcagg tgccgggact
     1741 gcgtctgtgt cggaggcgtt aattcttaca agctgctcaa tttcattcgg aggatcattt
     1801 tctgttaaat cctgtacatc accaaaatta gaatacaaca ctgccaatcc tcctttgtta
     1861 taaacatctg cgacaatagt ctttaaagga acacaagata atctccccgg aaaatcataa
     1921 taagtgaact ccgaaactgt ttgctgaaac aatgaatctg attttttatt caaaaacaaa
     1981 ttgattctgt cagctaaatt taccacaata tcattccatc cgtttttgtc aaaatcagcg
     2041 acagcaactg taaaaggact aaaagatcct gtatttgaat aagatggagt agtatttaaa
     2101 gtccctgaat tatttttata aatatttatt ccattatctt gttctactaa tacaagatca
     2161 ttgtatccgt cattattaaa atcagctatt ttaaaatctc tttttacaga gaatccaaac
     2221 cctgaataaa ttattgtcga taatgaagtt ccgttgttat tattgtttag atacactcta
     2281 atggaatctc cctgtattaa aatgatttca tctttgtctg atgttgtatt gtttaccgtg
     2341 gcgtacggtt cggcataact gctgatctga gcaagtgtaa cagcgctggc atgcatagga
     2401 agtctgtaaa tcggaataga atcaagataa ccgtttccaa ggtttttgta aatcttcaca
     2461 gtgtcatcca ttagtacagc tacatcttca agattatctc ctgttgtaaa atttcctact
     2521 gcagctactt ttccgtttgc tttaaagcct atacgaaata aaatagatcc aagactattt
     2581 gagtaaattt ccaaatcacc atttgctctt gatacaataa cttccttttt tacacttgga
     2641 cttgaactaa gttttgcaaa tataagacca gttataggat ttgaataatt acctaagtta
     2701 ataaatctac tagtttcgac ttcattattg aagttatcat tgttttcatt tttataccag
     2761 tttccgataa tgctgttacc tggtcctgaa ttaattcctt tctgtgatac gcctactaca
     2821 atatccaatc tgttattagt tgtatttacc tgacctaagt cccaataatc gaatttattt
     2881 gttacattcg gtatgatagt atcctcataa accggtactt ctacatagtc tccaaaataa
     2941 gtctgagcat acaaagatcc ggatgtaaaa tttattgcaa acagaataaa tatgaatatt
     3001 atatttcgaa ttattttatt tttctttttc atgattttat aagttttaat tttttttatt
     3061 aaaagctatg ttgattaaaa tttttataaa aaagagggaa agatcatcta tcttaagaat
     3121 taaa
//
LOCUS       DAORVQ010000297         3089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_27908_length_3089_cov_5.400132, whole genome shotgun sequence.
ACCESSION   DAORVQ010000297 DAORVQ010000000
VERSION     DAORVQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27908_length_3089_cov_5.400132"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLX80_14275"
     CDS             <1..422
                     /locus_tag="PLX80_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22005.1"
                     /translation="VRNNLILIFNLYYMFYKILKIFSVVLFLSVSTLILINSYKINLM
                     EENELSMTEKYLKHMVNDHKVNGSVFTFPVFNFIYINYFNRSSAFEMNLAAAVSLILI
                     PLTIIFYTGTAKLFCTDKILFMSGFNRSQFRPPRSYR"
     gene            598..2874
                     /locus_tag="PLX80_14280"
     CDS             598..2874
                     /locus_tag="PLX80_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQY22006.1"
                     /translation="MKKLIYILAAVILIQLNAVSILHSQNSVINGKVFDKETKLPLYG
                     AVITLVEINRSTSSNVLGDYNFSKLFEGSYKLKIELIGYEKVNLTIAVSGSDTVQTSA
                     YLIDNALTISQIDVTATIDYGSISVSPIDILLRPTSTAQDLLKLVPGLFIAQHAGGGK
                     AEQIFLRGFDIDHGTDINLQVDGMPVNMISHAHGQGYADLHFVIPETIDDFSVYKGPY
                     FSKFGDLGTSGSVDFKTKNSIEKSMGLLEFGSYDYYRALAMVKVLGENSHLFSKNKED
                     LYLAGEYAASNGYFDSRQKFSRLNVFGKYFGALSSKTILSVSGSTFYSHWDASGQIPE
                     RAVEENLIGRFGGIDNTEGSNTYRTNFNLNLMNSLSSNSSLTNQIYFSSYGFNLYSNF
                     TFFLNDPVNGDQINQFENRNIFGYKGTYSFLGQIGNVNLNTSFGIGARIDDIDTIHLA
                     HTRERSFLNYYEAGKILVQDYFAYMDESIEAGDFVINPGVRIDYFDFDLTDDLNSSNS
                     GSVSAVRIGPKLNLFYNLNNKTQFFIKSGIGFHSNDARVAVVENIGNTLPRAFGYEAG
                     TVFNAGNALINLSLWGLNLESEFVYVGDEAVVEPSGRTQRLGIDFSGRYQFNEWMWAD
                     LDLNYAKGKFTDEPEDADRIPLAPVFTSVGGLSARLDNGISGRIGYRYLDDRPANENY
                     SVTANGYFVMDAVLNLQFSGIYQIGLTFENLTNNSSWNEAQFDTESRLEFETDPVSEL
                     HYTPGTPFSLKGNFSINF"
BASE COUNT          992 a          508 c          596 g          993 t
ORIGIN      
        1 aagtcaggaa taatttgata ctaatattta atctttatta tatgttttat aagatcctta
       61 aaattttttc ggtagtttta tttctttccg tatcaacatt gatcctgatc aatagttata
      121 aaattaatct gatggaagag aatgaactct ctatgaccga gaaatattta aaacatatgg
      181 taaatgatca caaggttaac ggatctgttt ttacattccc ggtttttaat tttatataca
      241 taaattattt taaccggagt tctgcattcg aaatgaatct tgccgcagct gtaagtttaa
      301 ttttaattcc tcttacaata atcttttata caggcactgc aaagctgttc tgtactgata
      361 aaatcctttt catgtccggc tttaacagat ctcagttcag acccccgaga tcttaccgtt
      421 aacattccca tttcagcaat aaaaattttt aacaaaagcg caaagcaaca ggttatttat
      481 ttgaaagctc ttttattaac atgaaaataa ttttttttaa aattttgttt tcaattgacc
      541 tcctgtgcat ttttttaaaa atacttttga aaattaatac caactttaaa atcgatcatg
      601 aaaaaactta tatatattct ggcagcagta attcttatac agcttaatgc cgtcagcatt
      661 cttcattcac aaaattcagt cataaacgga aaagtatttg acaaagaaac gaaactccct
      721 ttgtatggag cggtaattac tttagttgaa attaacagat ccacatcttc aaatgtttta
      781 ggagattata atttctcaaa attgtttgaa ggaagttata aattaaagat agagttaata
      841 ggatatgaaa aagtaaactt gacaattgca gtcagcggca gtgacactgt tcaaacttcg
      901 gcatacctta ttgataatgc gctgacaatc tcgcaaatag atgtgacagc gacaattgat
      961 tatggaagca tctcagtaag tccgatcgat attcttctcc gcccgacttc aactgcccag
     1021 gatcttttga aactcgttcc gggattattc attgcccagc atgcaggtgg aggaaaagcc
     1081 gaacagatat ttctgcgcgg atttgatatt gatcacggga cggatattaa tcttcaggtt
     1141 gacgggatgc ctgttaacat gatatctcat gctcacggtc agggttatgc tgatctgcat
     1201 tttgtaattc ccgaaaccat agatgacttc agtgtttaca aaggtccgta cttttctaaa
     1261 tttggtgacc tcggaacatc aggctcagta gattttaaaa caaaaaattc aattgaaaaa
     1321 agcatggggc ttttggaatt cggcagttat gattattaca gagcgcttgc aatggtaaaa
     1381 gtgctcggtg aaaactctca tctgttttca aagaataaag aagatctgta tcttgcagga
     1441 gagtatgcag cttccaatgg atatttcgat tccaggcaaa agttcagccg gcttaatgtg
     1501 tttggtaaat atttcggtgc actctcatcg aaaacaatac tgtctgtttc aggatcgact
     1561 ttctattcac actgggatgc atcaggacaa attccggaaa gggctgttga agaaaatttg
     1621 atcggaagat tcggaggcat cgataatact gaaggcagta acacttacag aacaaatttt
     1681 aatttaaatc tgatgaattc attaagcagc aactcctcgc ttactaatca aatttatttt
     1741 tcaagctacg gatttaattt atattccaac tttacttttt ttctgaatga tcctgtcaat
     1801 ggagaccaga taaatcagtt tgaaaacaga aatatttttg gttataaagg gacttattca
     1861 tttttaggtc agattggaaa tgtaaattta aatacaagct ttgggatcgg cgcgcgtatt
     1921 gatgatatcg acacaattca tttagcgcat acacgtgaaa gatcgtttct taattattat
     1981 gaagcgggaa agatcctggt gcaggattat tttgcttaca tggatgaatc aattgaagca
     2041 ggtgattttg taataaatcc cggagtaagg attgattact ttgactttga tctgaccgat
     2101 gatctaaaca gctcaaactc aggcagcgta agcgcggtaa ggatcggacc aaagcttaat
     2161 ctgttttata atttaaataa taagacacag ttttttataa aatccggaat aggttttcat
     2221 tcgaatgatg caagagttgc agtagttgag aatatcggga acactttgcc gagagctttt
     2281 ggatatgagg ccggaactgt atttaatgcg ggtaatgcgc tgataaattt aagtctctgg
     2341 ggattgaatc ttgaaagtga atttgtttat gtcggagatg aagcagtagt agagccgagc
     2401 ggcaggacac agagacttgg gattgatttt tccggaaggt atcagtttaa tgaatggatg
     2461 tgggctgacc tcgatctaaa ttatgcaaaa ggaaaattta ctgatgaacc tgaagatgcg
     2521 gacaggatcc cgcttgctcc tgtgtttaca agtgtcggcg gattatctgc aagacttgat
     2581 aacggaatca gcggaaggat tggttacaga tatctggatg acagaccggc taatgaaaat
     2641 tattcagtca ctgcaaacgg atattttgta atggatgcgg ttctgaattt acagttcagc
     2701 ggaatttatc agataggtct gacttttgag aatcttacaa ataattcaag ctggaacgaa
     2761 gctcagtttg atacggagtc aagacttgag tttgaaactg atccggtatc ggaattacac
     2821 tatacacccg gaactccttt tagtttgaaa ggaaacttct caataaactt ttgatccggg
     2881 atttattcag tcagaaatag aattaatttt atttaaacaa aaaaaccgca gccgaataat
     2941 tcagctgcgg tttctcctgt tttacgcatc cggacaccct aatatttttg aagagatatt
     3001 ttttttatta acgttagaag tattttataa aaaaaatccc gcctgtaata cattgccgtt
     3061 aagaattctt gagaaattgt aaaaataaa
//
LOCUS       DAORVQ010000298         3086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_27970_length_3086_cov_3.472781, whole genome shotgun sequence.
ACCESSION   DAORVQ010000298 DAORVQ010000000
VERSION     DAORVQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27970_length_3086_cov_3.472781"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..905
                     /locus_tag="PLX80_14285"
     CDS             69..905
                     /locus_tag="PLX80_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22007.1"
                     /translation="MNPQKKFPVLKPEENLKFENDILKTKLTAEFGMKESDSSRLDAE
                     MENQCLNYIYDFENTCKNAGRITIYEFIGKPEFRSLGNEKLSVSETEAELDRILDVMA
                     ENGIMLDVLCDYEDEEELIYKFITEEFFPYEIDNIRIEGMNHCFTYEEFHTNHKYDLT
                     NDSEFLLDNIFGKEWYVQFSAACLACDIEYDSKNYSSEAFGKLVMDFQKSGKSYKLKS
                     KEVKEVEFDLEKGIANLKGIISYTIDLKNLSGEFEFDFIFNDYGYWNICKIVLPEDIL
                     VK"
     gene            1308..>3086
                     /locus_tag="PLX80_14290"
     CDS             1308..>3086
                     /locus_tag="PLX80_14290"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY22008.1"
                     /translation="MKKTLLLFYILFISITYVHSQSTWFWQQPLPTGNFLYAVDFLNE
                     NTGYAVGTVGTIIKTTNGGGNWVVQNSNFSSELLGMGFYDTELGFAVGGNNGSILRTS
                     NGGLNWTGVSSGTTTFMNDIDFPTRNTGYAVGLNGVILKSTNSGISWFALNSSTSASL
                     ICVSFLDSLNGVAGGNRIVLKTTNGGTSWISQAINFINFNPQVTGIDYIDSNTIISLA
                     NVDDRINKTTNGGLNWFGYEINLPELSDIPRNISFVNENTGYMVTSYGLILKSINGGI
                     NWSVDSTFKPSYYQIQVLQNVFALNENTSFVCGSGGRVIKSTDEGSNWMTTTGGRSNL
                     ESNFFVNENTGFTVGFDGVILKTTNAGNNWLQQNSNTTQNLNDVFFVNSETGYVCGDS
                     GVVLKTVDGGINWIGLVSGISTNLTSVFFQNQNTGFVVTSWVILKTENGGNTWFQTNS
                     NSGTRYEIFFADNFTGYVSTFDNFIKSTDGGNNWFNVPAASGSGMYFTNSMTGYSGGG
                     SGIIAKTIDGGNSLIVQNGNVSGNINSLFFVNNDMGYAVGNGGAITKTTNGGTNWISQ
                     AKISNNDFQSVFFTNYNTGFICGNFGA"
BASE COUNT         1048 a          425 c          646 g          967 t
ORIGIN      
        1 actaatttta taactgttga tataaaacat ttataaatta atttttttaa aattaaaaaa
       61 aggacgctat gaacccgcaa aagaaatttc ccgtcctcaa acctgaggaa aatttaaaat
      121 ttgaaaatga tattttaaaa accaaactca ccgctgaatt tggaatgaag gaaagtgatt
      181 catctaggct agatgctgaa atggagaatc aatgtctgaa ttatatttat gattttgaaa
      241 atacctgtaa gaatgccggt cgaataacca tctatgagtt tattggcaaa ccggaattca
      301 gatctttggg aaatgaaaaa ttatcagtga gtgaaaccga agccgaactt gacaggattc
      361 tagatgtaat ggcagagaac ggaatcatgc ttgatgtact gtgtgattat gaagacgaag
      421 aggagctcat ttataaattc ataaccgaag aattttttcc gtatgaaatt gataacatca
      481 gaattgaagg catgaatcat tgttttacct atgaagagtt tcacacaaac cataaatatg
      541 atctgacaaa tgattcggaa tttttacttg ataatatttt cggtaaggaa tggtatgttc
      601 aatttagtgc ggcctgttta gcttgtgata ttgaatatga tagtaaaaat tattcaagcg
      661 aggcttttgg aaagctggtt atggattttc aaaaatcagg aaagtcttat aaattaaaat
      721 caaaagaagt aaaggaagtt gaatttgatc ttgaaaaggg aattgcaaat ttgaaaggta
      781 taatcagtta cacaattgat ttaaaaaatc tatccggtga atttgagttt gattttatat
      841 tcaatgatta tggttattgg aatatttgta agattgtgct accggaagat atattagtta
      901 aatgaacacg tgtatttttt tagtaatgaa ctgagacaga gaatggatgg taagcagcgg
      961 agtccgttat aaggattgtt ctaactattc tgctgcggat tgaaatggtt atggatgagg
     1021 actttgatag tggtttcctt tttgaaagat gtatatgagt aaattgtaaa gtctcaaaat
     1081 aagtttgtgc ttaattctaa tcgcttatgg tttaatttca tgttgcttgc aacggggaga
     1141 ttcatttttg ttttattaat tatgttgtct cacaaatgaa cactttctca tggtactcat
     1201 tcatatcatt caagaccata gtcgctttca caaaccgcaa aaaataacgt ttgacatttt
     1261 aaattgaaaa tattataatg cagtcagaat ttcaaaattg aaggcttatg aaaaaaacac
     1321 ttttactctt ttatatacta tttataagta ttacatatgt acacagccag tccacctggt
     1381 tctggcaaca gcctttgccg acgggaaact ttttgtatgc agtagatttc ctgaatgaga
     1441 atacaggcta tgcggtcggg acggtaggta caatcataaa gacaaccaat ggcggtggga
     1501 actgggttgt ccagaattca aacttttcgt cagagctgct cggaatggga ttctatgata
     1561 ccgaacttgg ttttgctgtc ggagggaata atggctctat tctccgcact tcgaatggag
     1621 gactgaactg gacgggagtt tcttcgggaa caacaacatt tatgaatgat atagattttc
     1681 caacaaggaa taccggttat gcagttggac taaacggagt gatcttaaaa tcaacaaact
     1741 cagggatatc ctggtttgct ctgaatagtt ctacatctgc aagtttaatt tgtgtaagtt
     1801 ttttggattc tttgaatgga gttgcaggcg gaaacaggat cgtattgaag acgactaacg
     1861 gaggaacctc atggattagt caggcaataa attttataaa cttcaatccg caggttacag
     1921 gtatagatta tatagactcg aatacaataa taagtcttgc aaatgtagat gacagaataa
     1981 ataaaactac aaatggcgga ctcaactggt ttggctatga aatcaattta cctgaactca
     2041 gtgatattcc gagaaacatt tcttttgtaa atgaaaacac aggatacatg gttacgagct
     2101 atggtctgat cttgaaatct atcaatggcg gtattaactg gagtgtggat tcaacattca
     2161 aaccgagtta ttatcaaatt caggttttac aaaatgtatt tgcattaaat gaaaatactt
     2221 catttgtttg cggatcggga gggagagtaa ttaaatcaac agatgaagga agtaattgga
     2281 tgacaacaac aggcggccgg tcaaatctcg aatcaaactt ctttgtaaat gaaaatacag
     2341 gctttacagt cggattcgat ggagtaattt taaaaacaac aaacgccggc aataactggc
     2401 ttcagcaaaa cagcaataca acacaaaacc tgaatgatgt cttttttgtg aacagtgaga
     2461 cggggtatgt ttgcggggac tcgggagtgg tgctgaagac ggtggatggg ggtataaatt
     2521 ggataggtct ggtaagtggt atttcaacta atttaacaag tgtctttttc caaaaccaaa
     2581 acacaggatt tgttgttact tcatgggtta tactaaagac agaaaatgga ggaaatactt
     2641 ggtttcaaac aaatagtaac tcgggtacca gatatgaaat attttttgca gataatttta
     2701 caggttatgt ttctacgttt gataacttta ttaaatcaac ggatggaggt aacaattggt
     2761 ttaacgtgcc ggctgcaagt ggttcaggaa tgtattttac aaattctatg acaggatatt
     2821 caggaggagg atcaggaata attgcaaaga caattgatgg aggtaatagt ctgattgttc
     2881 aaaatggaaa tgtctcaggc aatattaatt ctttgttttt tgtaaacaat gatatgggtt
     2941 atgcggtagg aaatggagga gcgattacta aaactactaa tggaggaaca aattggatat
     3001 ctcaagctaa gatatcaaat aatgactttc agtcagtttt ttttacaaat tataatacag
     3061 gttttatatg tggtaatttc ggagca
//
LOCUS       DAORVQ010000299         3080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_28057_length_3080_cov_4.693554, whole genome shotgun sequence.
ACCESSION   DAORVQ010000299 DAORVQ010000000
VERSION     DAORVQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28057_length_3080_cov_4.693554"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PLX80_14295"
     CDS             <1..1458
                     /locus_tag="PLX80_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22009.1"
                     /translation="MVNSMIGYACALDGYIVKTTDGGLTWKNQISGSTENLNEIFFVD
                     SKTGYIACTAGTVLKTTDGGDNFNLAVISPNNGEVMISGSTSVIQWASTVSGNVNIEF
                     STNDGGNWNTIQNSVPANNFSFNWQVPSANSNTCKVKITSVENPSITDISDGKFAIVH
                     VNPFYNVPELIYYKFNDGINSTPNYAVPGQIYGDADINGMSLQNGGLADSSLVGQGGN
                     GLTNLVSTNWATNFPSSGFTIGFWVSNISLGTDPNNAVYLFADVTANNVRCYYGGAQG
                     LTGQDTAIMFRCGGMADVRIPVIQGQTYYIHIVFDPSVSQLRVFVNGTLTQTIPQGNV
                     LAIGNGPLKIGASANFSSSLANGMRLDEFRVYSRPLEDNEIAATWNITFPAVITGIQN
                     EEVFLRSDYMLFDNYPNPFNPETIIRYTLKEKGFTTLKIYNALGKEVSQLIGEFQNSG
                     NHSIRFNASDLSAGVYYYRLESGSFREAKKMVLIK"
     gene            complement(1608..2642)
                     /locus_tag="PLX80_14300"
     CDS             complement(1608..2642)
                     /locus_tag="PLX80_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22010.1"
                     /translation="MVNKNTAKRFYLFLILIPILFISACDDNINNNNEDLQEYDIDVW
                     DYSDNHYFLDTIYRSSFEDYFNNIGINSQTDSFSVDDNNFQVWIQTDVQTHDSRRAGL
                     HIDLSPMPTVGNYPDSLKSVLNPVQGVSASGLVRLLHSSEYVLNKYAGYISFKIDIPD
                     NIFAGVAYKRLRTGEKFGTNSTDSISSVDTLVLKMIKVQNLKPLNIIAWKMKLRNIYQ
                     LPAIGVSQDGFDFEIKYLKNGVYSTVLPINNLNQPLITIMGLDKYTNGRSGAPDGKFD
                     FLSGLTIDTENGWIIFPSLSPFVDKFSTPVNGITIDPIYWYPELYSQNKSDAKTFPNA
                     DYYLFSGLFK"
BASE COUNT         1033 a          494 c          509 g         1044 t
ORIGIN      
        1 atggtaaatt caatgatcgg ttatgcgtgt gcgctggatg gttacatagt taaaactact
       61 gatggcggat taacatggaa aaatcaaatt tcaggatcca cagaaaatct gaacgaaatt
      121 ttctttgttg attcaaagac tggttatatt gcctgtactg caggtaccgt tttaaaaact
      181 actgacggcg gtgataattt caacttagct gtaattagtc caaacaatgg agaagtaatg
      241 atttcaggtt cgacttcagt cattcaatgg gcaagtactg tatcaggaaa tgtaaatatt
      301 gaattttcta caaacgacgg cggaaactgg aacaccattc aaaattctgt tccggctaac
      361 aatttttcat tcaactggca ggttccttct gctaattcca acacctgtaa agtaaagata
      421 acttcggttg aaaatccatc tatcactgat atctctgacg ggaaatttgc gatcgtacat
      481 gtcaatccat tttataatgt tcccgaatta atttattaca aatttaatga tggaataaat
      541 tcgactccaa attatgcagt gccgggtcag atttatggcg atgcagatat aaacggtatg
      601 agtttacaaa atggcggact agccgacagc agtctggtag gtcagggagg taacggactg
      661 acaaatttgg tgtctacaaa ctgggctact aacttccctt cttcaggatt tacaataggt
      721 ttctgggtga gcaatatttc actcgggact gatccgaata atgcagtata tctttttgct
      781 gatgtaactg caaataatgt tcgctgttat tacggaggtg cacagggact tacaggtcag
      841 gatacagcga ttatgttcag atgcggagga atggcagatg taaggatacc tgtgatacaa
      901 ggtcagactt attacataca tatagttttt gatccttcag tttctcagtt aagggttttt
      961 gtaaatggta cattaactca aacaattcct cagggaaatg tacttgcaat tggaaacgga
     1021 ccacttaaaa taggtgcatc tgcaaacttc agttcatctc ttgctaatgg aatgagatta
     1081 gacgaattca gagtctacag tcgtccgctt gaagataatg aaattgcagc tacatggaat
     1141 ataaccttcc cggcagttat taccggtatt caaaatgaag aggtattctt aagatcagat
     1201 tatatgcttt ttgataatta tccaaatcct tttaatcctg aaactataat tcgttataca
     1261 ctaaaagaga aaggatttac aactttgaaa atttataatg cacttggtaa agaagtatca
     1321 cagctaatag gtgaatttca aaattcaggt aatcattcaa taagatttaa tgcatcagat
     1381 ttatctgccg gagtttacta ttacagactt gagtcaggaa gttttcgtga agcaaaaaaa
     1441 atggtcctca taaaataagg atattcgtct tttagggaat atttaattct attattgatc
     1501 ttcttatttc gaatcgttat tagtatttaa aaataattgc aaatttaaat attcaactga
     1561 tattctatat tattgtaatt attgcaaatt tttagaacac aaatttacta cttaaataat
     1621 cctgaaaata aatagtaatc agcattcgga aaagtttttg catctgattt attttgtgaa
     1681 tataattcag gataccagta tattggatca attgtaattc cgtttacagg agttgaaaat
     1741 ttatcaacga acggactcag tgacggaaat attatccagc cgttttcagt atcaattgta
     1801 agtccgctaa gaaaatcaaa cttaccatca ggagcacccg atcttccgtt agtatattta
     1861 tcaagtccca ttattgtaat taaaggttga ttcagattat tgataggtaa taccgtacta
     1921 tatacaccat ttttaagata ctttatttca aaatcaaaac catcctgaga aactcctatt
     1981 gccggcaact gataaatatt tctcagcttc atcttccatg caattatatt taatggcttt
     2041 agattttgaa ctttaatcat tttcagtact aatgtatcca cagaagaaat tgaatctgta
     2101 ctatttgtac caaacttctc tcctgttctt aatcttttgt aagcaactcc tgcaaaaata
     2161 ttatcaggga tatcaatttt aaatgaaatg taacctgcat atttattcag tacatattcg
     2221 cttgaatgaa gcaatcttac caatccggat gcactgacac cttgtaccgg atttagaacg
     2281 gattttaatg agtcaggata attaccaact gtcggcattg gggacagatc tatgtgaagt
     2341 cctgcccttc tagaatcatg tgtttgaaca tctgtttgaa tccagacctg aaagttatta
     2401 tcatcgacag aaaaactatc agtttgagaa tttattccaa tgttattaaa ataatcttca
     2461 aaactggatc ggtaaattgt atcaagaaaa taatgattat cagaataatc ccagacatca
     2521 atatcatatt cctgaagatc ttcattatta ttgtttatgt tatcgtcaca agctgaaata
     2581 aataatattg gaatgagtat aaggaaaagg taaaacctct ttgctgtgtt tttatttacc
     2641 attttatttt agttttaata aatgaattag taaagtagca attgagatat attgatctgt
     2701 ttaatttttg aaaaatttat ttgtcaattt gattataaaa tattcaaata tcaatctata
     2761 tttgtagata cttaaatttc ataaatgccg gagtttattc agactctata ttcccgctcg
     2821 tttcaaagct gccgcttgtt aacgtacttg aaattgaaac tccgtttcaa actcacaacc
     2881 aataattttt ctccagcggc aattcttgtc attgttggag tcctaaaaag ttctcgaata
     2941 attatattca attttatttt tgaataaaac gttttgcagg aatattataa ttttccacca
     3001 gtgttgtcca aaatatttaa atttcaaata aatagttcta aaattattga aaaatacaat
     3061 ttattaattt aaaaatgaaa
//
LOCUS       DAORVQ010000300         3059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_28362_length_3059_cov_3.931425, whole genome shotgun sequence.
ACCESSION   DAORVQ010000300 DAORVQ010000000
VERSION     DAORVQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28362_length_3059_cov_3.931425"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLX80_14305"
     CDS             <1..249
                     /locus_tag="PLX80_14305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HQY22011.1"
                     /translation="GMNVFQQEKSAKVSDFLKSKPVSYTVLDGNQEVVDAFGEASGNN
                     LEAVPTTFIIDKKGNIAETIVGSRKKEDFVKIINKYLN"
     gene            341..1495
                     /locus_tag="PLX80_14310"
     CDS             341..1495
                     /locus_tag="PLX80_14310"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQY22012.1"
                     /translation="MSVKVCIFGSTGSIGCNSLEVIDNLINKGKDYSIQYLTTNTNVD
                     LLAKQIKKYSPIGVAVTDDKSCEEFKRKYNFKDLEIFKGKAGILELAERDDFNLLISS
                     LVGISGLEPTIKAIKQNRKIALANKETLVVAGEIVNKLLKEKSCTLIPIDSEHSAIFQ
                     CLTGESKNNVSGIILTASGGPFLKKSLDEMNNASIKDALAHPNWNMGKKISIDSATMM
                     NKGLEIIEAKWLFDLTPDQISVVIHPQSIIHSMVEFNDGSIKAQLGIPDMKLPIQYAL
                     TYPDRIENNYPKMSFMENFNLSFEPPDNNKFKCLKLAVDALESGGIYPTVLNAANEIA
                     VELFLNNKIGFSQIPEIIENELNLTKSSSEINLETILETDNTIRNRLIAI"
     gene            1568..2938
                     /gene="rseP"
                     /locus_tag="PLX80_14315"
     CDS             1568..2938
                     /gene="rseP"
                     /locus_tag="PLX80_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQY22013.1"
                     /translation="MEFLTTTFYFLIVIGILVFIHEFGHFIAARMTGMRAEVFALGIG
                     YRLFGYNKINGFTFGSLSDEVILDDNTDYRICAFPIGGYVKVSGMIDESMDKDFISKE
                     PQPWEYRAKPVWKRMIVITAGVIMNFLLAFIIFYSLTLIKGKTLTDTTTIGYIGINSP
                     AAVSELVSGDKILKINNNEVNSWEDVQAKLYFENLGENLDFLVVRNGSETMVSIPKDK
                     TGDLTEKNFGIYPKGIQPEINDVVADMPASKCGLQKGDVIISYNNTEIKNPQQLTDLI
                     RSSVNDENEIHWTRNNENLNCVITPSADSTIGIMIAAKYTGPTKEIKYNIFTAAPKAV
                     YDMYYMGIELFFKTIAKIFRGDIAFKKAIGGPIKIAQVSAQSAEGGIYTFLGFIALLS
                     ISLAVINIMPFPALDGGHFMLLIYEAIVRKPVPYKVQLVLQNVGFAILIGFMIFVVYS
                     DITSLK"
BASE COUNT         1089 a          444 c          537 g          989 t
ORIGIN      
        1 ggaatgaatg tatttcagca ggaaaagtca gctaaagttt ctgatttttt aaaatctaaa
       61 cctgtatctt atacagtgct ggatggtaat caggaagttg ttgatgcatt tggtgaagct
      121 tccggaaaca atcttgaagc agttccgact acatttatta ttgataaaaa aggtaatata
      181 gcggagacta ttgtaggttc aagaaaaaaa gaagattttg taaaaattat aaataaatac
      241 ttaaattaat tcattaatat ttattttaag tgtataattc taaaactcaa ggattaaaat
      301 tcttgagttt tttatttatt cagcagcgaa ttaaaagtaa atgtccgtaa aagtttgtat
      361 atttggatcg accggttcta ttggatgtaa ttctcttgaa gtaatcgaca atcttatcaa
      421 caaaggtaaa gattattcca tacaatacct cactacaaat actaatgttg atttactcgc
      481 aaaacagata aaaaaatatt caccaattgg agtagctgtt actgatgata aaagctgtga
      541 agaattcaaa agaaaataca attttaagga tctggaaata ttcaaaggta aagccgggat
      601 tttggaatta gcagaaagag atgattttaa tttattaatt tcctcattgg ttggaatttc
      661 aggactcgaa ccaacaatca aagcaattaa gcaaaaccga aaaattgctt tagctaataa
      721 agagacatta gtagttgccg gtgaaattgt taataaatta ttaaaagaaa aatcctgcac
      781 tttaatccca attgatagtg aacattcagc aatttttcaa tgtctcactg gtgaatcgaa
      841 aaacaatgtt tcaggaatta ttctaactgc atccggtggt ccatttttaa aaaaatcatt
      901 agatgagatg aataatgctt ctataaaaga tgcattggca cacccaaact ggaatatggg
      961 taaaaagatc tcaatagatt cagcaacaat gatgaataaa ggacttgaaa taattgaagc
     1021 aaaatggctt tttgacctta ctcctgatca gatttcggtt gttattcacc ctcagtcaat
     1081 tatacattca atggtagaat ttaatgacgg ttctataaaa gcccagcttg gaattccaga
     1141 tatgaaactt ccaatccagt atgcacttac ttatccggat agaattgaaa ataactatcc
     1201 aaaaatgtca tttatggaaa acttcaattt atcttttgaa ccgcctgata ataacaaatt
     1261 caaatgtctc aaacttgcag ttgatgctct tgaatcaggt ggaatttacc caactgtttt
     1321 aaacgctgca aatgaaatag ctgtagaact ttttttaaat aataagatcg gattttctca
     1381 aattcctgaa attattgaga atgaattgaa tttgacaaaa tcgtcttccg aaatcaacct
     1441 tgagactatt ttagagacag acaatacaat tcgcaacaga ttaatcgcaa tataaatatt
     1501 taaaagagaa gtttttttaa ctctaatttt taattaataa attttatctt tactgtttaa
     1561 tatataaatg gaatttttaa caacaacatt ttactttctc atcgtaatcg gaattcttgt
     1621 atttatacat gagttcggac actttattgc tgcaagaatg acaggaatga gggcagaagt
     1681 ttttgctttg ggaataggat acagattatt cggatacaat aagatcaacg gatttacatt
     1741 cggctcgttg agtgatgaag taattcttga tgataataca gattacagaa tttgtgcatt
     1801 ccctatcggg ggctatgtaa aagtatcagg aatgatagac gaaagcatgg ataaggattt
     1861 tatttccaag gaacctcaac catgggaata cagagcaaaa cctgtttgga aaaggatgat
     1921 cgttatcact gcaggagtta tcatgaattt tcttctcgca tttataattt tttattcttt
     1981 aacacttata aaagggaaga ccttaactga tactacaact attggataca tagggataaa
     2041 ctctccggca gcggtgtcag aacttgtttc aggagataag atattgaaaa ttaataataa
     2101 cgaagtaaat tcctgggaag acgttcaggc aaaactttat tttgaaaatc ttggtgagaa
     2161 tctggacttc ttagttgtta ggaatggttc agaaaccatg gtttcaattc caaaggataa
     2221 aaccggtgat cttacagaaa aaaactttgg aatatatcct aaaggaatcc agcctgagat
     2281 caatgatgta gtagcagata tgcctgcttc taaatgtggt cttcaaaaag gtgacgttat
     2341 aatttcttat aataatacag aaattaaaaa tcctcagcag ctaaccgatc ttatcagaag
     2401 cagtgtaaat gatgaaaatg aaatccactg gacaagaaac aatgaaaatt taaactgtgt
     2461 aataactcca tcagctgatt ccactattgg aattatgata gcagcaaaat acaccggacc
     2521 tacaaaagaa ataaaatata atatttttac tgcggctccg aaagcagttt atgatatgta
     2581 ttatatgggt attgaattat ttttcaaaac aattgctaaa atattcagag gtgatattgc
     2641 atttaaaaaa gctattggcg gaccaataaa aattgctcag gtctctgctc agtcagcaga
     2701 aggtggtatt tatacctttc ttggatttat tgctttgctt tcaatttcac ttgcggtaat
     2761 taacattatg ccgtttcctg cattagacgg cggacatttt atgcttctta tatatgaagc
     2821 gatagttaga aaacctgtac catataaagt gcagttagtt ttacagaatg taggatttgc
     2881 aatattgatt gggtttatga tatttgtagt ttatagtgat attacatctt taaaataatt
     2941 tctttcattg tattcccagt ttgaaagcaa tatatttgtg ttgtaaattt ttcagctgaa
     3001 atatccggaa gggtgggtga gtggctgaaa ccaacggttt gctaaaccgt cgtacgggt
//
LOCUS       DAORVQ010000301         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_28490_length_3051_cov_5.208611, whole genome shotgun sequence.
ACCESSION   DAORVQ010000301 DAORVQ010000000
VERSION     DAORVQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28490_length_3051_cov_5.208611"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLX80_14320"
     CDS             complement(<1..588)
                     /locus_tag="PLX80_14320"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY22014.1"
                     /translation="MRKLFLIALITVLLLNSNLYAGWFGLQTGSNSYLTSIYFNNAST
                     GYVGGLNGYIAKTTDGGASWVQQNSLMNTQFVRAISFVDLNTGFICGDDGQIRKSTDG
                     GTTWIPQFSNTVAGIYCIEAVDANNVYAAVNNGTVLRSTDGGSTYFSVAVSPNQLLTL
                     DFVSIDTGFAAGQGGVVYKTTNAGLNWTFLNASTLN"
     gene            complement(843..1427)
                     /locus_tag="PLX80_14325"
     CDS             complement(843..1427)
                     /locus_tag="PLX80_14325"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HQY22015.1"
                     /translation="MSDILNGLLENLTGGQTPDKIASGLNIDPNIIKSLIPVIAPMIL
                     GGLKKQSEEHGGQERVDHILNKYGNPDILENVDSFVDVNSKQDSFDSNLGGLLGNSGS
                     QITDVLKEKFNLDAGTAAKIIPLVAPLILGYLTKSKNNENVGSAGFASFLDQNGDGSI
                     IDDVAGFFTNKQQDSNQGSGGGLMDILGGLLGKK"
     gene            complement(1665..3044)
                     /gene="egtB"
                     /locus_tag="PLX80_14330"
     CDS             complement(1665..3044)
                     /gene="egtB"
                     /locus_tag="PLX80_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HQY22016.1"
                     /translation="MKNIFMNYTNIDSHIKDIKIFEKTGTLFDEFIRVRNQTIEIVTP
                     LETEDYVVQTEAFMSPPRWHIGHLTWFYDQLLRKFYDGYKPFRENYDFYFNSYYLTFG
                     ELFNKSRRGTLSRPTVQETIEYWNYVNEQVFNFLNTVNGSLDENVRQLFILGFHHEYQ
                     HQELMVYDLQHLLSDKYIPVKMNVQPDNKSAIKIGEMIEIPGGIFEMGFDKNVYPALF
                     AYDIEMPLHKVYLNDFKIDKYPVTNGEYLDFINDGGYNNFKFWLSEGWESVKSNGWKA
                     PLYWQMNDNGNWSKIDFRGEKNITEIADEPVINVSYFEAAAFAKWAGKRLPTEAEWEK
                     AASFNENKKCKNQYPWGDDLPSSENSNTFDSRIWSPSKIGSYNNFKSSYGCEQMIGDC
                     WEWTSSEFMPYPGFKSGFEEYNDKWFNNQKVLRGGSFGTPGISTRNTYRNFFKTHERW
                     MISGFRCAV"
BASE COUNT          943 a          576 c          491 g         1041 t
ORIGIN      
        1 atttaatgta gatgcattca gaaaagtcca gtttaatcct gcatttgttg ttttataaac
       61 aacccctccc tgacctgctg caaatcctgt atctatcgat acaaaatcta aagtaagcaa
      121 ttgatttggt gaaactgcaa ctgaaaaata tgtactgcct ccgtctgtag agcgaagtac
      181 agttccgtta ttcactgcag catatacatt atttgcatca actgcttcta tacaatatat
      241 tcctgcaact gtattggaga actgcggtat ccaggtcgtc cctccgtctg tactttttct
      301 gatctgaccg tcatcaccgc agataaaacc ggtgttcaga tcaacgaatg aaattgctct
      361 tacaaactgt gtgttcatta aagaattctg ttgaacccag ctggcacctc cgtcagttgt
      421 ttttgcaatg tatccgttca gtcctcctac ataaccggtc gatgcattat taaaataaat
      481 tgatgtaagg taagaattgc tgccggtttg taatccaaac catcccgcat ataaatttga
      541 attgagaagt aaaactgtaa ttaatgcaat aagaaaaagt tttcgcatga taaggataag
      601 atgaattttt ataaatttaa aatggcatta aaataactat taataaaatt tcatcaaata
      661 ccatagttgt tgtatgcata cactataaat tgttatgaag tcttcattat ttagaaaaaa
      721 gaatgtgtat ttaagaaaat ttatttttag ttatttactt attgactaca aaaatgaatt
      781 aaaaaaaacg ccagaacaaa agtcctgacg ctttcaagta tattgaaatt tttttatctg
      841 aattattttt tgcccaacag tccgccaaga atatccatca gtccgccgcc tgaaccctga
      901 ttagaatcct gttgcttgtt tgtaaaaaat ccggcaacgt catcaatgat actgccgtct
      961 ccattctgat cgaggaatga cgcaaaacct gctgatccaa cattttcatt atttttactt
     1021 tttgttagat aacccagtat cagtggagca actaacggaa ttatctttgc tgctgttcct
     1081 gcatccaggt tgaatttttc tttgagaaca tctgtgatct gtgatcctga atttcccaga
     1141 agtccgccga gattggaatc gaaactgtcc tgtttggaat ttacatcgac aaagctgtct
     1201 acattttcaa gtatatcagg attaccatat ttattgagaa tatgatcgac tctttcctga
     1261 ccgccgtgtt cttcactttg ttttttcaaa ccaccgagta tcattggtgc aattacggga
     1321 attaaacttt tgataatgtt tggatcaatg tttaatcctg atgctatttt atcaggtgtc
     1381 tgaccaccgg ttaaattttc aagtaagcca ttaagtatat ctgacatttt ttactcctta
     1441 ttgttttagt ttataaatta ttgaaaatct ttttattctt tttcgacatt ccgcagagtc
     1501 caaagagcgc gtgactctgc ggatacaatt taaatatgac tctgctggta ctttgaaata
     1561 ttgaaaatct ttttattcta tttagacatt ccgcagattc cgaagatcac gtgactctgc
     1621 ggatacattg aaattattta atatttacta tttaagattt aaaattaaac cgcgcatctg
     1681 aatcctgaga tcatccatct ctcatgagtc ttgaaaaaat ttctgtaagt gttccttgtt
     1741 gaaattccgg gagtaccgaa agaaccgcct cttaaaactt tctgattatt aaaccacttg
     1801 tcattatatt cttcaaagcc ggatttgaaa cccggatagg gcatgaactc tgatgaagtc
     1861 cattcccagc agtcgccgat catttgttcg caaccgtaac tgcttttgaa attattataa
     1921 gaaccgatct ttgaaggaga ccagatcctt gaatcaaatg tgtttgaatt ttcagatgaa
     1981 ggaagatcat ctccccacgg atattgattt ttacactttt tattctcatt aaaagatgca
     2041 gccttttccc attcagcttc agtcggaagt cttttacctg cccattttgc aaaagcagca
     2101 gcttcgaaat aactgacgtt tattacaggt tcatcggcaa tttctgtaat atttttttct
     2161 ccgcgaaaat cgatcttcga ccagtttccg ttatcattca tctgccagta aagcggagct
     2221 ttccatccat tggattttac agattcccat ccttctgaaa gccagaattt aaaattgttg
     2281 tatccgccgt catttatgaa atcaagatat tcaccatttg tcacaggata tttatcaatt
     2341 ttaaaatcat taaggtaaac tttatgcaat ggcatttcaa tatcataagc aaacaaagca
     2401 gggtaaacat ttttatcaaa tcccatttca aaaattcctc cggggatttc tatcatttct
     2461 cctattttaa ttgcagattt attatcaggc tggacattca ttttaacagg aatgtattta
     2521 tcggaaagca aatgctgaag atcataaacc atgagttcct gatgctgata ctcatgatga
     2581 aatccaagaa taaatagctg tctgacattt tcatctaaac ttccatttac agtattcaga
     2641 aaattaaaaa cctgttcatt gacataattc cagtattcaa tagtttcttg gacagtcggt
     2701 ctggagagag tgcctcgtct ggatttatta aaaagttctc caaatgtgag gtaataggaa
     2761 ttaaaataaa aatcataatt ctctctgaag ggtttgtatc cgtcatagaa ttttctgaga
     2821 agctgatcat aaaaccatgt cagatgacca atatgccatc ggggcggact cataaaagct
     2881 tcagtctgga ctacataatc ttctgtttca agaggagtta caatttcaat tgtctggttt
     2941 cttacccgga tgaattcatc gaatagtgta ccggtttttt caaaaatctt aatatctttt
     3001 atgtgagaat caatattagt ataattcata aaaatatttt ttattgcaaa a
//
LOCUS       DAORVQ010000302         3050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_28504_length_3050_cov_5.904508, whole genome shotgun sequence.
ACCESSION   DAORVQ010000302 DAORVQ010000000
VERSION     DAORVQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28504_length_3050_cov_5.904508"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..2935
                     /locus_tag="PLX80_14335"
     CDS             383..2935
                     /locus_tag="PLX80_14335"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY22017.1"
                     /translation="MKKLFKLSLVVLISLMFSGEMMAQLNESFTGVTFPPTGWTRITY
                     LGTGGAFARTTTSSRTAPAAALSNFGSATGSENYLKTPQFTPIANDSIIFWFRQSFAT
                     VYQDTFTVLVSTTDTAKASFVPLLTLIDGVNYPTAATYGRFAASLSAYTGTPIYIAFR
                     HRDNDGDAVRIDDVSTLTPPPPGDNVGVSAAVFGSPLKILSAGKGYDLTATVTNFGTN
                     TQNVVPVYYTVNAGPAVGPVNTVGPILTSGTENVVFNGGNAFSPVTAGTYTLKVYTAL
                     VGDTNPANDTTTLTVTVLDKITAFPYTQNFTAPTGWTVTGGAAWGLAAAVNPDGVAAD
                     LAATANFYGIGAGITLILRSPEMDFSGMTNPVLDFYAAYRTYTGGESDTLRVMVSTDG
                     GVTFSPASTSYTKSWDSDPSLATLPPTGPSYTPTASSQWRHETVDLSNVGNMGNVVIG
                     FVGISDFGNNLFVDNVIISEPNSLCTDVVAAGGGIYSCNDIVSLNFTATPVPPINGNQ
                     ITSGVKKNEKAVFESFDNIPFYNGDAVINSFNQTDNPNGGTVFVSQYTMNDPGQTIEP
                     NATATAPDGSISTPTFVYSDFWFTITYDGNDQSGYATYDISMDITGAGFTIPNALYIV
                     KRTDNTAAWVCQNTTLAGNILTVSGLTDFSDFALAGMEALPVELSSFVSSINGSNVTL
                     NWSTASEINNSGFDIERSSVSGTWSKVGNVTGNGTSATTHSYTFTDRNLASGNYSYRL
                     KQVDFNGNFEYHNLSNEVIVGIPVKYDLSQNYPNPFNPSTKINYALPTDGQVSIKIFD
                     MAGKEVMSLVNEVKTAGYYSVNFNASNLSSGIYFYSLSAADFTATKKMMLVK"
BASE COUNT          974 a          545 c          547 g          984 t
ORIGIN      
        1 ttgaaattat ttttttggtt tggggaaagt tcgggatttt gtgtcgtgta atttatattt
       61 ttatgaaccc gttaaaaatc agtttttatt tcccgattta atttttatat tacattacac
      121 aggattaccc aaattaataa taaacaaaga aaggagatta gccttacaac gaattttact
      181 ctttttaaaa attcttcagg ttaaatttaa tatcaataca gttcattaaa ttagaagaca
      241 tatctgaaaa agtttttata tattccctgc agtaaattcc acgtgtttaa agtcttcaaa
      301 aacaacatca gtaatatcag caaaatttaa caacatcagt aaaatttaac aacattttat
      361 taatttaaaa caaactaaag ttatgaaaaa attattcaaa ctttctcttg tcgttttgat
      421 ttcccttatg ttttctggtg aaatgatggc gcaattgaat gaaagcttta caggtgtaac
      481 attccctccg actggatgga caagaataac ttatttaggt acaggtggag cttttgcaag
      541 aaccactact tcatcaagaa ctgccccagc tgcagcactt tcaaattttg gttccgctac
      601 aggaagtgaa aattatctta aaacaccaca gtttactcct attgctaatg attccattat
      661 tttttggttc agacaatcat ttgcaactgt gtatcaagat acatttactg tacttgtttc
      721 tacaacggat acagctaaag caagttttgt acctttactg acactaatag acggagttaa
      781 ttatcctacc gcggctacat acggaagatt tgcagcttct ttaagtgcat ataccggaac
      841 tcctatttat attgcattca gacatagaga caatgatggt gatgcagtta gaatagatga
      901 tgtttctact ctaactccgc ctcctccggg tgataatgtc ggtgtttcag cagcagtatt
      961 tggcagtcct ttaaagatct tatccgcagg aaaaggatat gacctgactg caactgtaac
     1021 aaacttcgga acaaatactc aaaatgtagt tcctgtttat tatacagtta acgcaggtcc
     1081 cgcagtaggt ccggtcaata cagtcggtcc gattcttacc tccggaacag aaaacgtagt
     1141 ctttaacgga ggaaatgctt tctcacctgt taccgcaggt acttatacac ttaaagtata
     1201 tactgcttta gttggtgata ctaatccggc taatgatact actactttaa cagtaactgt
     1261 tttggataag atcacagcat ttccttatac acagaacttt acagctccaa ctggctggac
     1321 agttacaggt ggtgctgcat ggggattagc agccgctgta aatcctgatg gagtagcagc
     1381 tgatctagct gcaactgcta atttttatgg cataggtgca ggtataacat tgattttaag
     1441 atcacctgaa atggatttct caggtatgac aaatcctgtc cttgattttt atgccgctta
     1501 tagaacatat acaggcggag aatcggacac attaagagtt atggtttcta cagacggcgg
     1561 agtgactttt agtccggcat ctacttctta tactaaatca tgggattcgg atccttcatt
     1621 agctacatta ccacctacag gtcctagtta tactccaact gcttcttcac aatggagaca
     1681 tgaaacagtt gatctttcaa atgtaggaaa tatgggaaat gttgttatcg gttttgttgg
     1741 tatctctgat tttggaaata atttatttgt tgataacgta ataatttcag agccaaacag
     1801 tttatgtact gacgttgtag cagcaggcgg tggaatatac agttgtaacg atatagtatc
     1861 tctgaatttt actgctaccc ctgttccgcc aataaacgga aatcaaatta catcaggtgt
     1921 taaaaagaat gaaaaagctg tttttgaatc atttgataat attccgtttt ataatggtga
     1981 tgcagtgatc aattctttca atcaaacaga taatccaaac ggaggaacag tattcgtatc
     2041 acaatataca atgaatgatc cgggtcaaac tattgaacca aatgctactg caactgctcc
     2101 tgatggttct atttctactc cgacatttgt atatagtgat ttctggttta ctattacata
     2161 tgatgggaat gatcagagcg gttatgctac ttatgatatc agtatggata taacaggtgc
     2221 aggattcaca attcctaatg cgctgtatat agttaaaaga actgacaata cagcagcatg
     2281 ggtatgtcag aacacaacac ttgcaggaaa tatcctgact gtatccggtc ttacagattt
     2341 ctctgatttt gctcttgccg gtatggaagc tttacctgtt gaattatcat ctttcgtatc
     2401 ttcaataaac ggaagcaatg taactcttaa ctggtctaca gcttctgaga taaataactc
     2461 cggatttgat atcgaaagat catcagtatc aggaacctgg tcaaaagttg gaaacgtaac
     2521 cggtaacggt acatctgcaa ctactcacag ctatacattc acagacagaa atcttgcatc
     2581 aggtaactac agctacagac tgaagcaggt tgacttcaat ggtaacttcg aatatcacaa
     2641 tttaagcaac gaagttattg tcggaattcc tgtaaagtat gacctttcac aaaactatcc
     2701 gaatcctttc aatccatcaa cgaagatcaa ttatgctctt ccgactgacg gacaagtaag
     2761 tattaagata tttgatatgg caggtaaaga agtaatgtca cttgttaatg aagtaaagac
     2821 tgcaggttat tattcagtca attttaatgc ttcaaactta tcaagtggaa tttacttcta
     2881 cagtttaagt gcagctgact ttacagctac aaagaaaatg atgctcgtga aataagttag
     2941 tagttaatag ttagtagtta ataatagaaa aactatttaa catttaaaat taaatattta
     3001 atatttgaaa gccgtgggat gaagaatcct gcggcttttt cttttaattt
//
LOCUS       DAORVQ010000303         3043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_28609_length_3043_cov_3.753012, whole genome shotgun sequence.
ACCESSION   DAORVQ010000303 DAORVQ010000000
VERSION     DAORVQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28609_length_3043_cov_3.753012"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLX80_14340"
     CDS             complement(<1..218)
                     /locus_tag="PLX80_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22018.1"
                     /translation="MLEDKDLFSKTENDFFLNENNSSVSVERICYNILWKTTPLLPTI
                     SDSRPNDTLKSKPAYVFHHLMKCGGTSVV"
     gene            complement(359..1576)
                     /locus_tag="PLX80_14345"
     CDS             complement(359..1576)
                     /locus_tag="PLX80_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22019.1"
                     /translation="MNKSNSTTNSSNTSNFLQFDFNDFSFSKKSHFRIFHEYNVDHTL
                     YGKRIEPDDSDIRIYQNLLIYTFVILNLDKGSKIIELGNDYSPVFEKLKLDYECWKFV
                     KPIELKNKFETIEDISDDLINKPDIKESEKLKLHYFDLVYSISALESLPESEVSLKET
                     ALNIRNFLKPMGFSINCFTNALNKDNVVWSHKLINYIYEKESTVNSFIDNSKIIHDND
                     LFIISEKYFNEYLKIHTGVTFQEFGKMYSYNVFWKNERYLSKDNFQEYTYSKKSHFGL
                     FNSYKSAESVYGKNTDELEYDIIKYQELLIYSYIKNNLKKGSKILKIGKSGGHLVDSL
                     KSDYVFYELECVNELSKMVFNKNNYDLNLIKDSNNNQLNIFPLNHFDFVLQLVPLMKL
                     KIVLRSMQLLLLI"
     gene            complement(1785..3041)
                     /gene="cysN"
                     /locus_tag="PLX80_14350"
     CDS             complement(1785..3041)
                     /gene="cysN"
                     /locus_tag="PLX80_14350"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HQY22020.1"
                     /translation="MDYQNNELLRFTTAGSVDDGKSTLIGRMLYDSKSIFEDQLEAVK
                     KSSEYKGLDYIDLSLLTDGLKAEREQSITIDVAYRYFSTPKRKFIIADTPGHIQYTRN
                     MVTGASTANLAVILVDARHGVVEQTCRHSLIASLLKISHIVLCINKMDLVNYDKDVYE
                     KIVADYKKFASKLEVKDIHFIPISALMGDNVVDRSEKMDWYEGSTLLYLLENVHIASD
                     NNHIDCRFPVQYVIRPQSDLNHDYRGYAGRIASGVFKPGDEVTVLPSGFTSKIKSIDT
                     FEGPVPEAFAPMSVTMRLENDIDISRGDMIVRANNQPEIGQDIEVIVCWLNNRPMNPS
                     AKYLLKHTTKDVKAIIKEVNYKFDINTLHRIEDDKEIKMNDIARLKLRTTHPLFYDSY
                     ERNRNTGSLIMIDEATKETVAAGMII"
BASE COUNT          999 a          503 c          416 g         1125 t
ORIGIN      
        1 actactgagg tgccgccaca tttcattaaa tgatgaaaaa cgtaagcagg ttttgatttt
       61 agtgtatcat ttggtctgga atctgatatt gtaggcagca gaggagtcgt tttccaaagt
      121 atattatagc aaattctttc aactgaaacc gaactattat tttcattcag gaaaaaatca
      181 ttttcggttt tagaaaacag atccttgtcc tcaagcataa atatatgcct gtgaaattta
      241 ttaaattttt ccacattctt aaaaaggaaa taaataaatg aattggattg agctacgcct
      301 ttataaaaaa cattattaaa actaaataat actaaggcaa attccttttt aaaagttgtt
      361 atattagtaa caatagttgc atagatctca agactatctt caatttcatc aaaggcacta
      421 attgtaaaac aaaatcaaaa tgatttaaag gaaaaatatt taattgatta ttattactat
      481 ctttaattaa attcagatca taattgtttt tattgaaaac cattttactt aattcattaa
      541 cgcactccaa ttcataaaaa acataatcac ttttaagtga atcaacaaga tgcccacctg
      601 atttaccaat tttaagaatt ttagaaccct tttttaaatt gttttttata tagctgtaaa
      661 ttaacagttc ctgatactta ataatatcat actccagttc atcagtattt ttaccataaa
      721 cagattcagc acttttgtaa ctgttaaata atccaaaatg cgatttcttt gaataagtat
      781 attcctggaa attatctttc gataaatatc tttcattctt ccaaaaaaca ttataagaat
      841 acatttttcc gaattcctga aatgtaactc cggtatgtat ttttaaatat tcattaaagt
      901 atttttcaga tataataaaa agatcattat catgaattat tttagaatta tcaataaaac
      961 tgtttacagt tgattctttt tcataaatgt aatttattaa tttatgtgac caaactacat
     1021 tatccttatt taatgcatta gtaaaacaat tgattgaaaa tcccataggt tttaaaaaat
     1081 tgcggatatt taatgctgtt tctttaaggg aaacttctga ttcaggcagg ctttcaagtg
     1141 cagaaataga ataaaccaga tcaaaataat gcaattttaa cttctcagat tccttaatat
     1201 cgggtttatt aattaaatca tctgaaatat cttcaattgt ttcaaattta ttttttagtt
     1261 caataggttt tacaaatttc cagcattcat aatccagttt taatttttca aacaccgggg
     1321 aataatcgtt tccaagttca attatctttg atcctttatc aagatttaat attacaaaag
     1381 tataaattaa taaattctga tatattctta tatcactatc atcaggttct atccttttgc
     1441 catataaagt atgatcaaca ttgtattcgt gaaatattcg aaaatgagat ttcttagaaa
     1501 aagaaaaatc attaaaatcg aattgtaaaa aatttgatgt atttgatgag tttgttgtgg
     1561 aattagattt attcaaatga atttaattta ttaagtttat atttgactaa attttattta
     1621 acatatttca atgtatgatt agaataaact tttgaattaa aaattaaatt taaattcaat
     1681 tatgattagt aatttgctta atgcttagga aagttttaaa aactaaaaat aaatatattc
     1741 cggtatatat ggtttattta atatgataac tgttttaaat taaatcaaat tatcatcccc
     1801 gcagcaacag tttcttttgt tgcttcgtca atcattatta aacttcccgt atttctgttt
     1861 ctttcgtaac tgtcataaaa cagcggatgt gttgtccgca gttttaatct tgcaatatcg
     1921 ttcattttaa tttctttgtc atcttcaatt ctgtgcagtg tatttatatc aaacttgtaa
     1981 ttcacttctt tgataattgc ttttacatct ttggtcgtat gtttaagcag atatttggct
     2041 gaaggattca taggtctgtt gttcagccag cagacaatta cttcaatatc ctgaccgatt
     2101 tccggctgat tatttgcccg tactatcatg tcacctctgc tgatatctat gtcattttcc
     2161 agacgcattg ttacagacat cggcgcaaat gcttcaggta ccggtccttc aaaggtatca
     2221 attgacttta tctttgaagt aaatccggaa ggaagtactg tcacttcatc acccggctta
     2281 aaaacaccgc ttgcgatcct tcccgcatat ccgcggtaat catgattaag atctgactgc
     2341 ggtctgatca catactgaac agggaatctg cagtcaatgt gattattgtc actcgcaata
     2401 tgaacattct ccaaaagata taacaatgta gagccttcat accagtccat cttttctgac
     2461 ctgtccacaa cattatctcc catcaaagcg cttataggta tgaaatgaat atcttttacc
     2521 tcaagctttg aagcgaattt tttatagtca gctacaattt tttcataaac atccttgtcg
     2581 taatttacca gatccatttt atttatgcat aaaacaatgt ggcttatctt tagcagtgaa
     2641 gcgatcaatg aatgtctgca ggtctgttcc actacaccgt gtctggcatc aacaagtatt
     2701 actgctaagt ttgcagtcga agcacctgtt accatatttc ttgtgtattg aatgtgacca
     2761 ggtgtgtcag ctatgataaa ctttctctta ggagtcgaaa aatatctgta tgcaacatca
     2821 attgttatac tctgttctct ttcggcttta agtccgtcag tcagtagcga aaggtcaata
     2881 taatcaagtc ctttgtattc gctcgacttt tttacagctt caagctgatc ttcaaatatt
     2941 gatttggaat cataaagcat tctgccgatc agcgtactct ttccgtcatc aacgcttccg
     3001 gctgttgtaa atctcagtaa ctcgttgttt tgataatcga tca
//
LOCUS       DAORVQ010000304         3017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_29082_length_3017_cov_4.416273, whole genome shotgun sequence.
ACCESSION   DAORVQ010000304 DAORVQ010000000
VERSION     DAORVQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29082_length_3017_cov_4.416273"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1260)
                     /locus_tag="PLX80_14355"
     CDS             complement(172..1260)
                     /locus_tag="PLX80_14355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY22021.1"
                     /translation="MVQRILHLSPDVGFNDGRSYYVFLLLKYLKRNGQKVFLCTNNTE
                     SRERILRYGAGFISIDTLSDKKKILNSISALSKIVKENKIEIIHSHHRYYEFLANAVR
                     LKTGVKTVFTALSIVDKRYFVEYKSDRLIAVSSYVKRMMVNSFGIEKERIQIIPNFTD
                     SEEITDNVDHDNESTEFLNIINDKKNKGYKILLSVGRYHKDKDHFTLLKALTQLKEKK
                     IFTVIIGSGDLKTEYEKFIKENDLNVLLVPAQKDLGKYYIAADICVLCSVKEPLPGFM
                     LQSGLFKKPFIGSDTDGIKEVIINNENGLIFKKRNYTELKDCIMMLTESSELPIALSE
                     KLHYLIIKDFTEKKTIPEIEMVYRSLIN"
     gene            complement(1271..2893)
                     /locus_tag="PLX80_14360"
     CDS             complement(1271..2893)
                     /locus_tag="PLX80_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22022.1"
                     /translation="MFLRSDRNKFFLSAFVIFLFFTIFLAISDYFSIGLLSDDYLNFI
                     SAKNSTLLQKFTSSIQNYSNFHLRPFWFLSIVNSINLNEMLGFEKSNFIFFRIENLIY
                     FYILTLLISYLLFKVTGNYIYSIILLLFCLMYPNNLNDICWTVGKVDILCAIFMFAAL
                     YVTYSFCEKNSDLKIAICLLFFLLALLTKESSVILPFISIIIIYISFGREKLHEIKYL
                     IFLEFFLLILYFAYRIIILGTQPVEVVTAYQNPGIIYSVGVIFKASVSMLIPFDYLSI
                     QDNLDLLNRNFLIYLIVSFSFLVTLLFIFSTTKSIGKLFLICLVFLSSILPNLIAGYF
                     RPQLILIPFVLIAFTTALVLFKLKYYYKFFLITILALLINWGIIGYKLVKNWDYSYDV
                     AEKVYAEFMNLDLDATKKNVILGLPSRLRQSYIMDYAIGPYNYYRHNEFEVKDKFFDL
                     VYIGALDSGSLNSELFVKKISDNEFEIYTTGETQYFMRLDAYSNKYKDRDLILKMTEE
                     NIFNKPTVMYLRILTENVNIILYSEGKFRKLN"
BASE COUNT         1041 a          476 c          425 g         1075 t
ORIGIN      
        1 agagtaaatt ttatttagag tgtcagcatt tgcatatgct atagttgcct ttttattatt
       61 aattacagct gcatttataa agaaatttaa tgaattgtaa tctgatttat caatctttat
      121 tccaaatatt ttaaaacttc cgctgatata attttttaat taattgaaaa attaattgat
      181 gagcgatcta tagaccattt ctatttcagg gatggtcttc ttttctgtga aatctttaat
      241 gatcagataa tgtaattttt cagatagtgc tataggtaat tcactgcttt ccgttaacat
      301 cataatacag tctttaagtt cagtgtaatt tcttttttta aaaatcaaac cgttttcatt
      361 attaattatt acttctttaa ttccgtcagt atcagaacca ataaatggtt ttttaaataa
      421 tccgctctgc agcataaatc cgggaagagg ttcttttaca gaacataata cacagatgtc
      481 agcagcaata taatatttcc caagatcttt ctgagcagga acgagcaaaa cattcagatc
      541 attttccttt ataaattttt catattctgt tttaagatca ccgctgccga taataactgt
      601 aaatatcttt ttctctttta gctgagtcaa tgcttttaac aaagtaaaat gatctttatc
      661 tttatgatat cgtcctacgg agagaagtat tttgtatccc ttgttttttt tatcattgat
      721 gatatttaaa aattcagtac tttcattgtc atgatctacg ttgtcagtga tctcttctga
      781 atctgtgaaa ttaggaatga tctgtattct ttctttctca attccgaagc tattgaccat
      841 cattcttttt acataactgc tgactgctat taatctgtct gatttatatt ctacaaaata
      901 tcttttatcc actatactca atgctgtgaa gacagtcttt acgcctgttt ttagtctgac
      961 tgcatttgca aggaattcat agtatctgtg atgactgtga atgatttcaa ttttattttc
     1021 ctttacaatt tttgataggg cagaaatcga attcagtatt ttttttttat cagacaaagt
     1081 atcaatagaa ataaatcctg ctccgtatct taaaatcctt tctctggatt ctgtgttatt
     1141 tgtacaaagg aatactttct gtccgtttct ttttaaatat tttaaaagaa gaaagacata
     1201 ataggatctt ccatcattga atccaacgtc aggagataaa tgtaaaatcc tttgcaccat
     1261 ttaaaataat ttaatttaat tttctgaatt taccttcgct atataaaatt atattcacat
     1321 tttctgtcag aattcttaaa tacattactg ttggcttatt aaaaatattt tcttcagtca
     1381 tctttaggat caggtctctg tccttgtatt tattactgta cgcatctagc ctcataaaat
     1441 actgtgtctc tccagttgtg taaatttcaa attcattgtc actgattttt tttacaaata
     1501 attcggaatt cagcgagcct gaatccaatg ctcctatata taccagatca aaaaatttat
     1561 ctttcacttc aaattcatta tgcctgtaat aattatatgg accaatggca taatccataa
     1621 tatacgactg acgcaatctt gacggcagcc caaggatcac atttttcttt gttgcatcca
     1681 gatccaaatt cataaattct gcatagactt tttcagcaac atcataagaa taatcccagt
     1741 tttttactaa tttataacca attattcccc aatttataag tagtgcaagt atggtgatga
     1801 ggaaaaattt ataataatat ttcagtttaa aaagtacaag agctgtagta aatgcaatga
     1861 gaacaaacgg tattaatatg agctgaggtc tgaaatatcc cgcaataaga tttggcagga
     1921 tcgaactcaa aaaaaccaga caaattaaga ataattttcc gatacttttt gtagtgctga
     1981 atataaataa cagagtaaca agaaatgaaa acgatacaat cagatatatc aggaaatttc
     2041 ggttaaggag atcaagattg tcctgtattg acagatagtc aaatggaatc agcattgaca
     2101 cggaagcttt aaaaattact ccaaccgaat agataatgcc gggattttga tatgccgtta
     2161 ctacttctac cggctgtgtt ccaagaataa ttattcggta tgcaaaataa aggatcaata
     2221 aaaagaattc taaaaatatc aaatacttta tctcatgaag cttttctctt ccaaaagaaa
     2281 tatagattat gataatcgag ataaacggaa gtatcacaga gctttcttta gtcagcagtg
     2341 caagtagaaa aaataaaaga catatcgcaa ttttgagatc tgaatttttt tcgcaaaatg
     2401 agtatgtaac atataatgca gcaaacatga aaatagcaca aagtatatct acctttccga
     2461 cagtccagca aatgtcatta agattgtttg gatacatcaa acagaaaagc agaagtataa
     2521 ttgagtatat ataatttcct gttactttga agagaagata agatataagc agtgtaagaa
     2581 tgtaaaagta aatgagattt tctattctga aaaaaatgaa attgcttttt tcaaatccca
     2641 acatctcatt aagattgata ctgtttacaa tgcttaaaaa ccaaaatggt cttaaatgaa
     2701 aattgctgta gttctgaatc gaacttgtga atttctgaag taacgtagaa ttctttgcag
     2761 atataaaatt cagataatcg tctgacaata gtccaatact aaaataatca gaaatagcca
     2821 gaaagatcgt aaagaataga aaaattacga atgctgataa gaaaaactta tttctatctg
     2881 atctcagaaa caatttattt attttaaatt ctgtaaatta atcagctctt gtttaaagaa
     2941 ctttttattt aatttgaaag actcaatcct ctgccttagt ctcatatctc tcacccggtt
     3001 aaaatatttt tcaggca
//
LOCUS       DAORVQ010000305         2998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_29408_length_2998_cov_5.132178, whole genome shotgun sequence.
ACCESSION   DAORVQ010000305 DAORVQ010000000
VERSION     DAORVQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29408_length_2998_cov_5.132178"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..961)
                     /locus_tag="PLX80_14365"
     CDS             complement(23..961)
                     /locus_tag="PLX80_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQY22023.1"
                     /translation="MKQFGISVHGGAGTILKSLMTPEKESSYRNSISESIRQGSYILR
                     SGGSSIDAVIKAITILEDDPNFNAGKGSVFTNEGKHSMDASIMNGNDLSAGSVASICG
                     VRNPILLAKAVLDVKDLVLINGNGAIEFAKEKGLKFEDDEYFYSQHRYDQYLKALKKG
                     KAQLDHTEDEITDKKFGTVGAVALDTNGNLAAATSTGGMTNVKFGRIGDSPVIGAGTY
                     ANNNTCAVSCTGDGEFFLRSVTAFDVSAVMEYKLLTLKKACKEVVHKKLISIGGEGGL
                     IAIDKDCNIEMVFNSKGMYRGFCKNDGELVVKIYND"
     gene            complement(1035..2051)
                     /locus_tag="PLX80_14370"
     CDS             complement(1035..2051)
                     /locus_tag="PLX80_14370"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQY22024.1"
                     /translation="MSKTIFIVLLSVILFQDGFSQGSGNKYAGEWLQIGVGGRPKGMG
                     GAYVALVDDVTSGYWNPGGLAKINYPQFSLMHDAQFGNLVNYDYGSVGIPFGASSSLG
                     FSIIRMGIDDIQDTRNALIDLNGNGTLDPGERIDESRISYFNTSDYAFYLTYSKVSNE
                     QLSYGANFKVLRRNLGDQGAWGLGFDVGVKYSPFNNFFLGANLMDITTTYLSWSTGKN
                     EVITPTAKIGTAYQVEVFKGTLTPVVDFDLRFENRQASANAYIGPMSLDIHAGIEYAF
                     KDVVAIRTGVNDLGNLTLGAGVKLPKINIDYSFAKFDGLEELGNSHIVSLVFTLEEEK
                     FQRK"
     gene            2739..>2998
                     /locus_tag="PLX80_14375"
     CDS             2739..>2998
                     /locus_tag="PLX80_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY22025.1"
                     /translation="MSKKLILPISIILIALGIFVGMKIQDAVSDDKISSQVKKFNDAL
                     EVTSRYYVDDVDSQKLTEDAIKGMLESLDPHSVYISADQLKRV"
BASE COUNT         1028 a          519 c          422 g         1029 t
ORIGIN      
        1 aaagtgattt taaattaaat aatcagtcat tataaatttt gacaactaat tctccgtcat
       61 ttttacaaaa acccctgtac attcctttag aattgaaaac catttctata ttacaatctt
      121 tatcaatagc aatcaaaccg ccttcaccgc ctattgagat cagcttcttg tgaacgactt
      181 ctttacaggc ttttttcaat gtgagtaact tgtattccat gacagcagat acatcaaaag
      241 ctgtgacact tcttaaaaaa aattcgccgt cacctgtaca tgaaacagca caagtattat
      301 tgttagcata tgtaccggca ccaataactg gactatcacc gattctgcca aatttaacat
      361 tggtcattcc tccggtagaa gtagctgcag ccagattccc gtttgtatca agtgcaacag
      421 ctccaacagt tccgaacttt ttatctgtga tctcatcctc tgtatgatcg agctgtgcct
      481 tacctttttt taatgctttt agatactgat catatctgtg ctggctgtaa aaatattcat
      541 catcttcaaa cttcaatcct ttttctttgg caaattctat agcaccatta ccgttaatta
      601 atacaagatc tttaacatcc aatacagctt ttgcaagaag tatcgggttt cgtactccgc
      661 aaattgaagc aacacttccg gctgaaagat catttccatt cataatggaa gcatccatgc
      721 tgtgcttgcc ttcatttgta aaaacagatc ccttgcctgc gttgaagttt ggatcatctt
      781 cgagaatagt aattgctttt attacggcat cgatagacga accaccggaa cgtagtatgt
      841 agcttccctg acggattgat tcactgattg aatttctgta tgaagattct ttttcaggag
      901 tcataagtga tttcaaaata gtacctgctc ctccatgaac tgatattccg aattgtttca
      961 taatttattt attgaataaa aaaaaacctt gctaaattaa attaacaagg tttatataaa
     1021 ctaaactcta taatttattt tctctgaaat ttttcttctt caagagtaaa cacaagtgaa
     1081 actatatgtg agttacctaa ttcttcaagt ccgtcaaatt ttgcaaatga ataatcaatg
     1141 ttgatctttg gtaattttac gcctgctcca agtgtaagat ttcctagatc atttacacca
     1201 gttctgattg ctacaacatc tttaaaagca tattcaatac ccgcgtgaat gtcaagactc
     1261 attggtccaa tatatgcatt agcacttgcc tgtctgttct caaatctaag gtcaaaatca
     1321 acaacaggag taagtgtgcc tttaaaaact tcaacctgat aagctgttcc gatttttgca
     1381 gttggtgtta ttacttcatt tttacctgtg gaccatgata gataagtagt ggtaatatcc
     1441 attaaattag caccgaggaa aaaattatta aaggggctgt attttacacc aacatcaaat
     1501 ccaagtcccc aggctccctg atctccaaga tttcttctca atactttgaa atttgcaccg
     1561 taggataatt gttcatttga aacttttgaa taagtgaggt aaaaggcata atctgaggta
     1621 ttgaaatagg aaattcttga ttcatcaatt ctttctcctg gatcaagagt gccgttccca
     1681 ttcaggtcga tcaaagcatt tcgggtatcc tgaatatcat caattcccat tctgattata
     1741 gaaaatccaa gtgatgagct tgcaccaaat ggaattccaa ctgaaccata atcataatta
     1801 acaagattac cgaattgagc atcatgcata agagaaaact gcgggtaatt tatctttgca
     1861 agtccgccgg gattccagta gcctgaagtt acatcatcta cgagagcaac atatgctcct
     1921 cccattcctt ttggtctacc accaactccg atttggagcc attctcctgc atacttatta
     1981 ccacttcctt gagaaaatcc atcttgaaaa aggataacag aaagcaaaac tataaaaata
     2041 gtttttgaca ttagattatt cataaattgg gtttgtttac ttttaaattg ttaaatacag
     2101 taaactaatt ttcattatcc tccggataaa aatttttttg taataatgat tattttattc
     2161 cgataagtca atacaaaatt aattgatttt agataaaagt gctatattgg tattaaagtt
     2221 tcagcgaaat ttctacagat aaaaataaat atttattgtt ttctcaatta aatgagaatg
     2281 ttttattttt taaattttta ataatttcta tttagaattg aataaaagta agtgccaaat
     2341 tttaaattaa tggaaaatat cagtttagat aaaaatacca atcatctttt caaattaaaa
     2401 aaaatattaa aattcatcta aagtttagtt aaaagtaaat tggttacgaa aatattatta
     2461 ttatataaag tgaaaattta ttgcaaaata atataatcat ccttctttat taataaaaat
     2521 aaaaaacaat taattttaac ttaatgagct aagctgacgt aatacatttg aaaacttaat
     2581 aatacatatt attaaatttc gatttataag gaacaataaa ccattcaata gtatttctta
     2641 taatatctta ataatgtatt tttattaaga actttcaaat aattcaaatc aatttaaaat
     2701 catttttaaa ttaatcaaaa taatttctat tttaaactat gagtaaaaaa ttaattttac
     2761 ccatttcaat aatactgata gcacttggta tttttgtagg gatgaaaatt caggatgctg
     2821 tttctgatga taaaatttct tcgcaggtca agaaattcaa tgatgcgctg gaagtaacat
     2881 caagatatta tgtagatgat gtcgattcac aaaaactaac agaagatgca attaaaggaa
     2941 tgcttgaaag tctagatccg cattctgttt atatttcagc cgatcagcta aaaagagt
//
LOCUS       DAORVQ010000306         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_29638_length_2985_cov_4.660068, whole genome shotgun sequence.
ACCESSION   DAORVQ010000306 DAORVQ010000000
VERSION     DAORVQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29638_length_2985_cov_4.660068"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PLX80_14380"
     CDS             <1..1388
                     /locus_tag="PLX80_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY22026.1"
                     /translation="ESLDPHSVYISADQLKRVNEDFQGSFEGIGVEFDIVNDTLTIVS
                     PISGGPSEKLGIQAGDKIVKIDGISSVEISREDVPKKLKGPKGTKVNVTIVRAGLPDQ
                     LEFEITRDKIPIYSVDASFMYDDEVGYVKVSRFSATTYDEFMKAMGDLKNQGMKKVVL
                     DLRGDPGGYLDQAFKMASEFIPAGNKIVYTKSRIKDFNEEYVSEGGKYNDIPLVVLVN
                     GGSASASEIVSGAVQDWDRGLIVGETTFGKGLVQRQFDLPDGSAFRVTTARYYTPSGR
                     LIQKSYDGSNYKETANADVEGDNIEHSNDIQDSARPEYTTFGGRKVFGGGGITPDYII
                     KLDTLTDYSVQLRRLNLFYIFTERYMGANRSNIESKYKTADEFKNGFIVDEVMLTDLK
                     NMATEKDVTFNQDEYNKDLEFIKTSIKSQIARDLWGNNGIYIVWLENDEQFLKGVSLF
                     EESIKLAKLKQ"
     gene            1397..2224
                     /locus_tag="PLX80_14385"
     CDS             1397..2224
                     /locus_tag="PLX80_14385"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQY22027.1"
                     /translation="MILNILRRSFISAILIIGIIANEAFSQDNVFETGEELKYEISYG
                     FIKLGYMKFVLTNTKKTGKKTFYNARLEVKTYPEVPFITVNDIFETEMEFSEVEEKDE
                     EELFSNKFYETNFRNRSITRQDCRFNYKKYMVKYSKETDGDLEIDKEIPIKEHIRYRD
                     ELSWMYEYRLNSFTNKNYNIPVFSEGEGSSVRYSYNVNKTVIKIEKTNYEIAVIKMEG
                     TSDFTGVFGFKGEFLVLLSDDDYRVPIKAYFNSSLGNVVLELIEYKKAKWTPPPFLK"
     gene            2314..>2985
                     /locus_tag="PLX80_14390"
     CDS             2314..>2985
                     /locus_tag="PLX80_14390"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQY22028.1"
                     /translation="MSLFNVAMSQSKVLQVGEELNYTVNYGFIEIGNVTIKLTGKKTV
                     GSNEIYTSTCTMRSNKGIPFVDLNSNFESEMLYNGNELYTIRFKATEIKDDGKIEIEY
                     KFHYDSNYVYVLKNNLGNIEIDKNIPFNNNVKFQDGLSLFFKSRLNSFSSDNFMIPVF
                     MNESETSVNYYFNSQKEEIFVPVFDEDLNSFRCNGVANFEGVFGLSGEFAGWFSDDEA
                     RIPLKS"
BASE COUNT         1114 a          371 c          576 g          924 t
ORIGIN      
        1 ttgaaagtct agatccgcat tctgtttata tttcagccga tcagctaaaa agagttaacg
       61 aagatttcca gggaagtttt gagggaattg gagttgagtt tgatattgtt aacgatactc
      121 ttactattgt atctccaatc agcggcggtc catctgagaa gcttggaatt caggcaggtg
      181 ataagatcgt aaaaattgac ggaattagca gcgtcgaaat ttccagggaa gatgtgccta
      241 agaaacttaa aggaccaaaa ggtacaaaag taaatgtaac aatagtaaga gccggattac
      301 cggatcagct ggagtttgag ataaccagag ataaaattcc aatttatagt gtcgatgcat
      361 catttatgta tgatgatgaa gttggttatg taaaagtatc aaggttttcc gcaacgacat
      421 atgatgaatt catgaaagca atgggtgatc ttaaaaatca gggtatgaaa aaagtagtac
      481 tcgatcttag aggagatccg ggcggatatc tagatcaggc ttttaaaatg gcaagtgaat
      541 ttattcctgc aggtaataaa atagtatata caaaaagcag gataaaggat tttaatgaag
      601 aatatgtatc agaaggcgga aagtataatg atattccttt ggtcgtatta gttaatggag
      661 gttctgcatc tgccagtgag attgtttccg gtgcagttca ggattgggac agaggattaa
      721 ttgttggtga aactactttt gggaaaggtc ttgtgcagag acaattcgat ctgcctgatg
      781 gttcagcttt cagagttact acagcaagat attatacgcc ttcaggaaga ctgatacaaa
      841 aaagttatga tggaagcaat tataaagaaa ctgcaaacgc agatgtggaa ggtgataaca
      901 ttgaacattc gaatgacatt caggattcag caagacctga atatactact tttggcggta
      961 gaaaagtatt cggcggaggt ggaataactc ctgattatat aattaagctt gatactctga
     1021 cagattattc tgtacaactg agaagattaa atttatttta tattttcact gagagatata
     1081 tgggtgcaaa cagaagcaat atagaatcaa aatataaaac agctgatgaa tttaaaaacg
     1141 gatttatagt agatgaagta atgctgactg accttaaaaa tatggctaca gaaaaagatg
     1201 taacatttaa tcaggatgaa tataataagg atttagaatt tataaaaact tcaataaaat
     1261 cgcagatagc aagagatctg tggggaaata acggaatata tattgtatgg cttgagaatg
     1321 atgaacaatt cttaaaagga gtttcattat ttgaagaatc gatcaagtta gcaaagttaa
     1381 aacaataaaa tcagaaatga ttttaaatat cttaagaaga agcttcattt cggcaattct
     1441 gataatcgga ataattgcga atgaagcttt ttcacaggat aatgtttttg aaacaggtga
     1501 agaattaaag tatgaaattt cgtacggatt tataaagctc ggatacatga aatttgtcct
     1561 tactaacaca aaaaagactg gtaaaaaaac attttacaat gccagacttg aagtaaaaac
     1621 ctaccctgaa gtaccattca taactgtaaa tgatattttt gaaaccgaaa tggaattcag
     1681 tgaagtagaa gaaaaagatg aggaagaatt gttctcaaat aaattttatg aaactaattt
     1741 cagaaacaga tctattacac gtcaggactg cagatttaat tataaaaaat acatggttaa
     1801 atattcaaaa gaaactgatg gtgatcttga aattgataaa gagattccaa taaaagaaca
     1861 tatcagatac agagatgaac tttcatggat gtatgaatac agattaaatt cgtttacaaa
     1921 taaaaattat aatatcccag tcttttcgga aggggaagga tcatcagtaa gatattctta
     1981 caatgtaaat aagacagtta taaaaataga aaaaacgaat tatgaaatag cagttataaa
     2041 aatggaagga acatccgatt tcacaggagt gtttggtttt aaaggcgagt ttttagtgct
     2101 tctttcggat gatgattata gagttccgat taaggcatac tttaattctt ctcttggaaa
     2161 tgttgtgctt gaattaattg aatacaaaaa agctaaatgg actcctccac catttttaaa
     2221 ataaataaaa tcaataaagt gaaatttcaa tttataaata aaatatacaa atattcgaac
     2281 attttaattt ggtgtatttc tttattttcg tctttgtctt tatttaatgt tgcaatgtct
     2341 cagtcaaaag ttttacaggt cggggaggag ctaaattaca ctgtaaatta tgggtttatt
     2401 gaaatcggaa atgtaactat aaaactcact ggaaagaaaa ctgtcggatc aaatgaaatt
     2461 tatacttcaa cctgtacaat gagatcaaac aaaggaattc catttgtaga tctgaacagt
     2521 aattttgaat ctgaaatgct ttataatgga aatgaattat atactattag attcaaggct
     2581 acagaaatta aagacgatgg aaaaatagag atcgagtata agttccatta tgattctaat
     2641 tacgtttacg ttttaaaaaa taatttgggg aatattgaaa ttgataaaaa tatacctttc
     2701 aataataatg taaaatttca ggacggtctt tctttgtttt ttaagtcacg actaaattca
     2761 ttttcatcgg ataattttat gattccagta tttatgaatg aatcagaaac ttctgtcaat
     2821 tattatttta attctcaaaa agaagaaata tttgttccag tatttgatga agatttaaat
     2881 tctttcagat gtaacggagt agccaatttt gaaggagttt ttggtcttag cggtgaattt
     2941 gcaggttggt tttcagatga tgaagcacgc attcctttga aatcc
//
LOCUS       DAORVQ010000307         2952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_30217_length_2952_cov_4.129444, whole genome shotgun sequence.
ACCESSION   DAORVQ010000307 DAORVQ010000000
VERSION     DAORVQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30217_length_2952_cov_4.129444"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLX80_14395"
     CDS             <1..185
                     /locus_tag="PLX80_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22029.1"
                     /translation="LIAVKIFSELILLRNTFRIFNVKYLYKYYPLFTIYFAVYGLLLP
                     ITFIFKSGIKWKERKF"
     gene            complement(182..883)
                     /locus_tag="PLX80_14400"
     CDS             complement(182..883)
                     /locus_tag="PLX80_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22030.1"
                     /translation="MKNKIFSVLMLLFLMINLSDVTYSQNVLSKDYFKNYNFLKDGKN
                     KLNKDDSSKIISGKNSVKKVNSGGIFLAPYIGYAIPTGLFGDQSNTGFLWGVKVELAY
                     SKLYPLIFGFIYENQSIPGNPEFTTTNSLTSFETDMNNIGGSVDLLLNKYIKSNFTSL
                     ILSVEVKYSKVVRTITSNIAQLEELPGDENLLIWTAGLGFTIYVFDLGGRYTYAEKFS
                     NISFLMRIHIPVVKF"
     gene            complement(900..1820)
                     /locus_tag="PLX80_14405"
     CDS             complement(900..1820)
                     /locus_tag="PLX80_14405"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HQY22031.1"
                     /translation="MNKRIYIIGAGKVGSAFAFELSAIGYHVNFLTDRNPDNLERVSK
                     KLIPLVSSESVDKNFIESSDIIFICVQDRFIKNVLKEISDSGADISGKYFIHTSGSES
                     SALFLNENFRESFTGSMHPIQTFDSVSTENKHLLSEIYFGIEGDKDCVELFKVIAKEL
                     ESNILEIPADKKFLYHSACVVSSNFLVTLMNISAELLGSIGIDKSISFEIFKPIVKNT
                     IENISENGLVNSLTGPFERNDIETISNHLNSIYKELPSLIPFYTLLGMETVKVAFKKE
                     SINLNSVISMLDLMNSYVVNEVKTDKNKIN"
     gene            complement(1813..2271)
                     /gene="dtd"
                     /locus_tag="PLX80_14410"
     CDS             complement(1813..2271)
                     /gene="dtd"
                     /locus_tag="PLX80_14410"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQY22032.1"
                     /translation="MIAVIQRVSNAEVKIEGKVHSSIGPGLLVLLGISKGDTENELKY
                     IARKLTEIRIFADENDKMNLNIKDINGEIMLISQFTLCAETKKSGNRPSFTSAESPEI
                     AEILYEKTKSELANLYSSEKIKSGIFAAKMNISLTNLGPVTILLEKKFNE"
     gene            complement(2268..>2952)
                     /gene="prmC"
                     /locus_tag="PLX80_14415"
     CDS             complement(2268..>2952)
                     /gene="prmC"
                     /locus_tag="PLX80_14415"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQY22033.1"
                     /translation="ISRRISNEPLQYILGRTNFFGYDISLDRNVLIPRQETELLVERV
                     LEDISASGKKSVSIFEIGTGSGCIAIALGKKLGEQNISYEIFSIDKSEKALNIAKKNL
                     ELNGISGKEITLYCRDVFDIPRLKKNYDYIISNPPYISIAEWEKLPDEIRNYEPKTAL
                     TDDKNGLNFYSKIFEIFSDLEFSGKLFCEIGYRQRESLEVLLRKYEIMKYKFYKDYSD
                     IFRILKAEK"
BASE COUNT          973 a          504 c          378 g         1097 t
ORIGIN      
        1 tactgatagc agttaaaata ttttccgaat taattttact cagaaatact ttcagaatat
       61 ttaatgtaaa atacttgtac aaatactatc ccctgtttac tatttacttt gcagtatacg
      121 gactcttgct tccaattaca tttatattta aaagcggtat aaaatggaaa gaaagaaaat
      181 tctaaaattt tactacagga atgtgaattc tcattaagaa agaaatattt gaaaattttt
      241 ctgcataagt atatctgccg cccaaatcaa atacataaat tgtaaatcca agtccagccg
      301 tccagatgag caaattttca tctcccggaa gttcctcaag ctgtgctata ttggatgtaa
      361 tagtccttac tactttcgaa tattttactt cgacagacaa gatcaatgat gtgaagttag
      421 atttgatata tttattaaga aggagatcga cactacctcc gatattattc atgtcagttt
      481 caaaacttgt caatgagttt gtagttgtaa attcaggatt acctgggata gattgatttt
      541 cataaataaa tccgaatatc agaggatata gcttgctgta tgcaagttca actttaactc
      601 cccacaaaaa acctgtatta gactgatctc caaaaagacc tgtcggtatt gcatatccaa
      661 tatatggagc aagaaaaatt ccacctgaat ttactttttt aacactattt tttccggaaa
      721 ttatttttga agaatcatct ttatttaatt tgttttttcc atccttgagg aagttataat
      781 tcttaaaata atcttttgat aaaacattct gtgaatatgt tacatcagat aaatttatca
      841 ttaaaaacaa taacattaaa actgagaaga tcttattttt caaaaaaaga ttttattaat
      901 tagttaattt tatttttatc agtttttact tcatttacaa catagctatt cataaggtca
      961 agcattgaga taacactgtt aagatttatt gactcttttt taaaagcaac tttgacagtt
     1021 tccattccga gtaatgtata aaatggtatt agtgatggaa gttctttata tatagaattt
     1081 aaatgattgg aaattgtctc aatatcattt ctttcaaatg gacctgttag tgaatttacc
     1141 agtccgtttt cagagatatt ttcaatagtg ttttttacta ttggtttaaa gatctcaaaa
     1201 gaaatacttt tgtcaattcc gatagatcca agtaattccg ctgaaatatt catcagtgtt
     1261 actaagaaat ttgaagacac tacgcaagct gaatgataaa gaaatttttt atcagcagga
     1321 atttcaagta tatttgattc aagttctttt gcaataactt taaataactc cacgcaatct
     1381 ttgtctcctt caattccaaa ataaatttca gaaagcagat gtttattctc cgtactgaca
     1441 gaatcaaatg tctggatagg atgcattgaa ccggtaaaac tttctctaaa attttcattt
     1501 aaaaaaagag cagatgattc tgaccctgat gtatgaataa aatatttacc ggatatatca
     1561 gcacctgaat cagaaatttc ttttaataca ttttttataa atctgtcctg tacgcagata
     1621 aagattatat cagaagattc aatgaaattt ttatcaactg attctgaaga taccaatgga
     1681 attaattttt tagatactct ttccagatta tccggatttc tgtctgttaa aaaatttaca
     1741 tggtacccga tagcacttaa ttcaaacgca aatgcagatc caacctttcc tgcacctata
     1801 atgtaaattc ttttattcat taaatttttt ttctagtaaa attgtaacag gtccgagatt
     1861 tgtcaaagag atattcatct ttgctgcgaa gataccgctc tttatttttt cggaactgta
     1921 taaattagct aattcagatt tagttttctc atataaaatt tctgcaattt ctggagactc
     1981 tgcagatgta aaactcggtc ggtttccact cttctttgtc tcagcgcaaa gtgtgaattg
     2041 tgaaatcagc attatctcac cgtttatatc ttttatattt aaattcatct tatcattttc
     2101 atcagcaaaa attcttatct ctgttaattt tctcgcaata tactttaatt cattttcagt
     2161 atcaccttta ctgatcccaa gtaataccag taaacccggc cctatactac tatgaacttt
     2221 accttctatt tttacttcag cattagatac tctttgaatc acagcaatca tttttcggct
     2281 tttaatattc tgaaaatatc gctgtaatct ttataaaatt tatatttcat tatctcatac
     2341 tttcttaata aaacctcaag actttccctt tgtctgtatc cgatttcaca aaacaattta
     2401 cctgaaaatt ctaagtcact aaatatttca aatatcttac tataaaaatt taatccgttt
     2461 ttatcatcag tcaaagcagt cttaggttca taatttctga tctcgtccgg gagtttttcc
     2521 cattcagcta tagaaatata tggcgggttt gagattatgt aatcataatt cttttttaat
     2581 ctgggaatat caaatacatc tctacaataa agagtaattt cttttccgct tattccgttt
     2641 aattccaaat tctttttggc aatattaagt gctttttcag atttatcaat agaaaatatt
     2701 tcataagaaa tattttgctc acctaatttt ttccctaaag caatagctat acatcctgac
     2761 cctgttccaa tttcaaaaat gcttactgat ttttttccac ttgcggaaat gtcttccaaa
     2821 actctttcga caagtaattc agtttcctgt ctgggtatca atacatttct gtcaagactt
     2881 atatcataac caaaaaaatt cgtcctgccc agaatatact ggagcggttc attactgatc
     2941 ctcctcgaaa tt
//
LOCUS       DAORVQ010000308         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_30359_length_2944_cov_6.432330, whole genome shotgun sequence.
ACCESSION   DAORVQ010000308 DAORVQ010000000
VERSION     DAORVQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30359_length_2944_cov_6.432330"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2218
                     /locus_tag="PLX80_14420"
     CDS             <1..2218
                     /locus_tag="PLX80_14420"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQY22034.1"
                     /translation="DNGILKGKIIDNETEAPVEGAVIEILRTKFKTGTNSEGEFYFDN
                     LKYDSYQIQITAAGYEPMVRSDLVLYASKPLELTVKIKPKGYKTSVIDVEGNFFIQSS
                     DVNLSSINLDYEEIRRAPGATEDISRMLQSAPGIAIGNDQRNDIIVRGGSPAENLIII
                     DGIDIPNINHFGTEGSSSGAIGFINSKFIFETTMLTGGFPSLYGDRLSGVVDISFREG
                     NRKSFYGDINLSISGFGAIIEGPVTENGSFLFSVRRSYLELIQDAIRLTSVPNYWDFN
                     LKAVYDLSPNDRLTLIGLAGIDKIDFSGESAENNPFGNSDDDQKIYALGLNYKKLFRN
                     GYLETVVSNTYLNNYIIQIDGQTADVKFRNTADNNEITVRSNLNYQLSETLLLNTGIG
                     TKLAGINNEIFLRGDTSAAGYAYDTINTNTSFNTYKLFAHGNLSYKLFDDRLILNTGI
                     RVDYFDYLRLPTYFSPRIGASYLLSPITSLNAAWGIYYQSPEYIWLSASPENFRLNDI
                     RCEHYIAGIDHYLASDLKATVEFFYKQYKDYPVWQDIPTYILIDGGADFGPNIVGEAV
                     SAGNGSTEGFDISLQKKLSNTGLYGLLSYSYIHSGFTALAGGEKPGAFDPGSQFTLIA
                     GYQFNDGWLAGFKFKYSGGRPYTPINPVASKQVNRAVYATDDFNSARYPYYLRVDVRI
                     DKKFNFKSSTLVAYLEIQNLFDRQNIYAYYWNEDYKSLETIYQWAFFPVGGISMQF"
     gene            2399..>2944
                     /locus_tag="PLX80_14425"
     CDS             2399..>2944
                     /locus_tag="PLX80_14425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQY22035.1"
                     /translation="MKDSSELVSVLIPARNEERYIEKCIRNVMMQTYSNLEIVIYDDS
                     STDGTNSIVTKTSKEDKRVKIINGESLPDGWRGKNFACWNLSKHAEGNYFLFIDADVT
                     MEKDAVGSALYEIQNKNADILSVFPKQIILSFGEKAVVPFLNWLLISFIPVKFISSPK
                     YKSLAGANGQFILWKKETYEKS"
BASE COUNT          950 a          521 c          574 g          899 t
ORIGIN      
        1 agataatgga atattaaaag ggaagataat tgacaatgag actgaagctc ctgtcgaggg
       61 agcggttatt gaaattttaa ggacgaaatt taagacaggg acaaattccg aaggtgaatt
      121 ttattttgat aatctgaaat atgattcata tcagattcag atcactgctg ccggatatga
      181 accgatggtt agatcagatc tcgttttata tgcaagcaaa cctcttgaac ttacagtaaa
      241 aataaaacca aaaggatata aaacttctgt aatagatgtt gaaggaaatt tttttataca
      301 aagctcggac gtaaatctca gctctattaa tcttgactac gaggagatcc gccgtgcacc
      361 gggtgctacg gaagatattt cccgtatgct gcagtctgct ccgggcattg ccattggtaa
      421 tgatcagaga aatgatatca tagtcagagg cggatcacct gcagaaaatc ttattattat
      481 agacggcatc gatattccga atataaatca ctttggcaca gaaggttcat caagcggcgc
      541 aatcggattt ataaattcca aattcatttt cgaaacgacc atgctgaccg gcggatttcc
      601 gtctttatac ggagacaggc tttcaggtgt ggttgatatt agttttcgcg agggaaaccg
      661 aaaatcattt tacggagata taaatctttc catttccgga ttcggagcga tcatcgaagg
      721 accagttaca gagaacggtt cattcctttt ttctgtaagg agaagttatc tcgagcttat
      781 tcaggatgcc atccgtctta cgtctgtccc gaattactgg gactttaatc tcaaagctgt
      841 ttatgacctg agtccgaatg acaggctgac acttatagga cttgcaggaa ttgacaaaat
      901 tgatttctca ggtgagtcag ccgaaaataa tccgtttgga aattcagatg acgatcagaa
      961 aatatatgct ctcggattaa attataaaaa acttttccgc aatggttatc tcgaaacagt
     1021 ggtttcaaat acttatctga ataattatat aattcagatc gatggacaaa ctgctgacgt
     1081 gaaattccga aacactgcag acaataatga gatcactgtc agatcaaatc tgaattatca
     1141 gctttctgaa acgctgctgc tgaacaccgg tatcggcaca aagttagccg gcataaacaa
     1201 tgaaattttt ctcaggggtg atacaagtgc tgcaggttat gcttatgata caattaacac
     1261 aaacacttct tttaatactt ataaattatt tgcgcatgga aatctttctt acaaattatt
     1321 tgacgacaga ctgatcctga atacaggtat cagagttgat tactttgact atctaagact
     1381 gccgacttat ttttcaccgc gcatcggagc ttcttacctg ctctcaccga tcacttcatt
     1441 aaatgctgca tggggaattt attatcagtc acctgaatac atctggctca gtgccagccc
     1501 tgaaaatttc cggcttaatg atatcagatg cgaacattac attgcaggca ttgatcacta
     1561 tttagcgagc gatctcaaag ccactgtcga atttttttac aagcagtata aagattatcc
     1621 tgtatggcag gacattccga cttatattct gattgatggg ggagcggatt tcggacctaa
     1681 tattgttggt gaagcagtaa gtgcaggtaa cggttcgaca gaaggttttg atatttcatt
     1741 acaaaaaaaa ttatccaata ccggacttta tggtctttta agttattcat atatacactc
     1801 aggatttaca gcacttgctg gcggagaaaa gcccggtgca ttcgatccgg gaagtcagtt
     1861 cacgcttatc gccggatacc agttcaatga cggctggctc gccggcttca aatttaaata
     1921 ttcaggcggc agaccttaca cgccaatcaa tcccgttgct tcaaaacaag tgaaccgtgc
     1981 cgtgtatgct actgacgatt ttaacagtgc aagatatccg tattatttaa gagtagatgt
     2041 cagaattgat aaaaaattta attttaaaag ttcgactctc gttgcttatc ttgagataca
     2101 aaatctattt gaccgccaga atatatatgc atattattgg aatgaagatt ataaaagtct
     2161 tgagactatt tatcaatggg ctttctttcc tgtaggcgga atcagtatgc agttctgatt
     2221 tattaatttg tctgcctcta ctttccgaaa tcgaattgca aattttatct gctaaataaa
     2281 ataaattata gtacatctgg aaataattat tctaataata ttatctgtaa taccggttta
     2341 cactttttta ataagcatta taaatctttt tacagctccg tttcttaaga aaaatgtagt
     2401 gaaggattcg agtgaactag tatcggtact gataccggca cgcaatgaag agaggtatat
     2461 agaaaaatgt ataaggaatg ttatgatgca gacatattca aatctcgaaa tagtaattta
     2521 tgacgattca tcaactgacg ggacaaattc aattgttaca aaaacttcaa aagaagataa
     2581 aagagtaaag ataattaatg gagagtcatt accagatggc tggaggggta aaaatttcgc
     2641 atgctggaat ttatcaaaac atgctgaggg taattatttt ttatttatag atgccgatgt
     2701 aacaatggaa aaggatgcag tcggttcagc tctttatgaa attcaaaata aaaatgcaga
     2761 cattttatct gtattcccaa aacaaataat tttaagtttt ggggaaaaag ctgttgttcc
     2821 atttttaaac tggctgttaa tttcttttat tcctgtaaaa tttatttcct ctccgaagta
     2881 caaatccctt gccggcgcta acggacagtt cattttatgg aaaaaagaaa catacgaaaa
     2941 gtca
//
LOCUS       DAORVQ010000309         2935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_30541_length_2935_cov_7.865625, whole genome shotgun sequence.
ACCESSION   DAORVQ010000309 DAORVQ010000000
VERSION     DAORVQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30541_length_2935_cov_7.865625"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2900)
                     /locus_tag="PLX80_14430"
     CDS             complement(<1..2900)
                     /locus_tag="PLX80_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22036.1"
                     /translation="MILAFGLSDKNTDKNLDKKSDRKSDNEPKIISDRNNNPSYTASV
                     DSKKMDGNNISTWYRNNGSFNRNPSTGNSGLEYPKGAAKFATYASGVWIGAIVANDTL
                     MAIAEYNYEYLPGYIDDNGNPQGKDDPLYKIYKIYKGDTLSTDYQNWPLSQGAYADEN
                     GKPFMMGDMTMFYSYTDGYPEAHNNNAGSTAPLKVQILQTNWCYNSISSYLNDVIITE
                     MRIINRSDQAWTKCYMALWSDNDIGTATDDAIGCDTNLNLGYGYNFGIIDPDYGSTPP
                     AVGILLLKGQYVQSPGDTIKYYSPPLSNNLIIKPGNKDLGMTSLNMIINSNPIIGDPS
                     NVKEAYRIIQGVIKNGQPWINPLNNQITPFVYSGDPVTQAGWIESSGGNKRLILSSGP
                     STVNPGDTQTIVYAQIISRSSSNLQSLNSLKINSHYLKSLYDNNFNITISAAPPVAKS
                     YSSGNGKVYLSWNDSAERIKYPNKYSNTDFKFQGYNIYQISSYSANPSESDTVLIKTF
                     DIKDGIKNIFDSVYYSEQQGYLYGIVQKGSDNGISRYIELDKDSLGNQNFINGAGYKY
                     CVTAYYYDPAGGPFTFPKVLESPKIVLKVIPQNLTSGTTINYSLGDTVITSQKDLGVT
                     PVIIDPVKLLNASYTSVFGMTGNNKLGWTLTRNFNGNSEVLFQNIEDFTGKQDTAKTA
                     DGLMFIHNTVKDSGIVIDPDDNYYESQNIPTYSIQKAWTYEPAENNLFRGPDTSAVIT
                     AKVFTNRQFDSRSLGMSFPTQARFNNFRSRVIANGKYFTHGTGTNTLPMGGPLRKIKI
                     VFGENSKAYRYAPPVNVIQTDTNLLATLYTDFTDVPFSVYAADELDSTLGNPRRLNIG
                     FVDADANGLWDPDDSPLGKYQLTYIFASDYSEVINQNYVSRSGQPINIGSGNPAFGFS
                     ALDVMYAWLPRKKSASSTWINGDVLTVSPYRITRSDFVPGFPLKYSWTINGST"
BASE COUNT          843 a          579 c          514 g          999 t
ORIGIN      
        1 gtagatccgt ttattgtcca tgaatatttc agtggaaagc ccggcacgaa atcagatctc
       61 gtaattctgt atggagaaac tgtaagtaca tcaccgttta tccatgtact tgacgcggat
      121 ttttttctcg gcagccaggc atacataaca tcaagcgcag aaaatccaaa tgcaggattt
      181 cctgaaccaa tatttatagg ctgtcccgac cttgaaacgt aattctgatt tatgacttca
      241 ctgtaatcgg atgcaaaaat atatgtcagc tgatattttc ccaaaggaga atcatccgga
      301 tcccaaagac catttgcgtc agcatctacg aatccaatat tcagtcttct tggatttcct
      361 aaagtagagt cgagttcatc agctgcatat acactgaaag gcacatctgt aaaatctgta
      421 tatagagtag caagtaaatt tgtatcagtc tgaataacat ttacaggcgg agcatatctg
      481 taagctttag aattttcacc aaatacaatt ttgatctttc tcaaaggtcc gcccattggc
      541 agagtattcg tgcctgttcc atgtgtgaaa tattttccat tcgcaattac tctcgatctg
      601 aaattattaa atcttgcctg ggtcggaaaa ctcattccaa gactgcggct gtcgaattgt
      661 ctgtttgtaa atacttttgc agtaattact gctgatgtat ccggtcctct gaacaagttg
      721 ttttcagcag gttcatatgt ccatgccttt tgaattgaat aggttggaat attttgactt
      781 tcataataat tatcatcagg atcaataact attcctgaat ccttaactgt attatgaatg
      841 aacataagtc cgtccgctgt ctttgctgtg tcttgctttc cggtaaagtc ttcaatattt
      901 tgaaataaga cctcactgtt tccgttaaaa tttctcgtca gtgtccatcc aagtttgtta
      961 ttacctgtca ttccaaatac agatgtataa gatgcattta ataattttac aggatctata
     1021 ataaccggag ttactccaag atctttctga cttgttataa ctgtgtcacc taaagaataa
     1081 ttgatggtag tacctgaagt taaattttga ggaatgacct tgagaactat cttcggcgac
     1141 tcaaggactt taggaaatgt aaacggtccg cctgccggat cataataata tgctgtcaca
     1201 caatatttat acccggcacc gtttatgaaa ttctgattgc cgagtgaatc tttgtcaagt
     1261 tcaatatacc gtgagatgcc gttgtcactg cctttctgga ctataccata aagatatccc
     1321 tgctgctcag aataatacac actgtcaaaa atattcttta tcccgtcttt tatatcaaaa
     1381 gtctttataa gcactgtatc gctttcggac gggtttgcag agtatgaact tatctgataa
     1441 atattataac cctgaaattt gaaatctgta tttgaatatt tatttggata ttttattctc
     1501 tctgccgaat cattccatga gagatagacc ttaccgttgc ctgacgaata agacttcgca
     1561 acaggcggag ctgcagaaat agtaatgttg aaattgttat cgtacaaact tttaagataa
     1621 tgcgaattaa ttttcagaga atttaaactc tgaagattgc tgctgcttct tgaaattatc
     1681 tgtgcatata ctattgtctg agtatcgccc ggattaacgg ttgacggtcc ggaagacagg
     1741 ataagtcttt tattaccgcc actggattcg atccatcccg cttgtgttac cggatcacct
     1801 gaatatacaa atggagtgat ctgattattt aacggattga tccagggttg gccattttta
     1861 ataacacctt gaattatacg gtaagcttcc ttgacattgg atggatcacc tataatggga
     1921 ttcgaattta taatcatgtt aagtgatgtc atgccaagat ctttattgcc aggctttata
     1981 atgagattgt tactaagagg cggactgtaa tattttatag tgtctcccgg actttgaaca
     2041 tactgtcctt tcaataaaag aataccaact gccggaggag tgcttccata atccggatca
     2101 attattccaa agttatatcc atatcccaga tttaaatttg tatcacaccc aattgcgtca
     2161 tcagttgcag ttcctatatc attatcactc cataaagcca tatagcattt tgtccatgcc
     2221 tgatcacttc ggttaataat tctcatctct gtaataatta cgtcattcag atatgaactt
     2281 atgctgttat agcaccagtt ggtctgaagt atctgtactt ttaacggtgc tgtgctgccg
     2341 gcattgttat tgtgagcttc gggataaccg tcagtataag aatagaacat tgtcatatca
     2401 cccatcataa acggcttacc gttttcatca gcgtatgccc cctgactcaa cggccagttt
     2461 tgataatctg tacttaatgt atctccttta tatattttat aaattttgta aagcggatca
     2521 tcttttccct gcggatttcc attgtcatca atgtagcccg gaaggtattc ataattatat
     2581 tcagcaatag ccatcaaagt atcgttagcg acaattgccc cgatccatac tccggatgca
     2641 tatgtagcga attttgcggc acccttcgga tattctaatc ctgaattacc tgtagaagga
     2701 tttctattga aacttccgtt atttctgtac catgtactta tattatttcc atccattttt
     2761 tttgaatcta cacttgctgt ataagatgga ttgttatttc tatcagaaat tattttcggt
     2821 tcattatctg atttcctgtc tgatttttta tctaaatttt tatctgtgtt tttatcacta
     2881 agcccgaatg ctaagatcat gaatgcacaa atcgaaataa ataccggtaa actaa
//
LOCUS       DAORVQ010000310         2910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31031_length_2910_cov_5.276357, whole genome shotgun sequence.
ACCESSION   DAORVQ010000310 DAORVQ010000000
VERSION     DAORVQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31031_length_2910_cov_5.276357"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1217
                     /locus_tag="PLX80_14435"
     CDS             39..1217
                     /locus_tag="PLX80_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY22037.1"
                     /translation="MQSSGTYKSLDYFGIDEMLTDEERSIRDTVRDFVNEEVLPIIEE
                     YNQKMDFPKQLIPKMAELGIFGPTLPVEYGGMGINNVAYGLIMQELERGDSGVRSFAS
                     VQSALVMYPIYTFGSEEQRKKYLPKLASAEMIGCFGLTEPDYGSNPGGMITKAEKVDG
                     GYILNGAKMWITNGTVADVAVVWAKLDGKVKGFLVDKGTKGFSAPEMKGKLSLRASIT
                     SELVFEDCFIPDENLLPESGGLKSPLMCLTQARYGIAWGVIGLANECYSTVLNYSLSR
                     IQFDKPLAAFQITQEKLAYMVSEITKAQLLCFQLAKLKDAGKMRPQQVSLAKRNNCEI
                     AKIIASMSREMLGANGILDEYPIMRHQNNIESVKTYEGTHEMHTLIIGEDVTGIAAYK
                     "
     gene            1340..2572
                     /locus_tag="PLX80_14440"
     CDS             1340..2572
                     /locus_tag="PLX80_14440"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQY22038.1"
                     /translation="MKTFDIPIFYRSPVISKIKNSRKLNDPRKKDYSPTELDFGPVVF
                     YIARHFGFCYGVENAIEISYKAIEENPGKRIFLLSEMIHNSDVNSDLIERGVKFIMDT
                     SGMQLIDWNELHSEDIVIIPAFGTTLEIERKLNELNIDPLKYNTTCPFVEKVWNRADS
                     LGKTGFTIIVHGKYKHEETRATFSHSKESAPTLIIRDITEAEILSEFITGKRDIKEFY
                     EIFANRYSEGFDPVKDLNSIGVVNQTTMLATETQAIADYLNKIMSEKFGTEKFSEHFS
                     DTRDTLCYATHDNQKATYGLLEKNADIAIVVGGYNSSNTSHIYELCEEKLPTYFISSA
                     DKILSKELISHFDFQNQKEIFTDNFLPVKDKVKIILTSGASCPDSIVDEVLQKLLSYF
                     NSTKKIDLVLEEFSMNKN"
     gene            2601..2759
                     /locus_tag="PLX80_14445"
     CDS             2601..2759
                     /locus_tag="PLX80_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HQY22039.1"
                     /translation="MSLFRVILAIILPPLAVIDKGCGSVVIVAILTLIGWVPGVIAAL
                     IILNKNES"
BASE COUNT          996 a          458 c          570 g          886 t
ORIGIN      
        1 taattaacaa ttaaataatc aaaaaggaat attagaaaat gcagtcatca ggaacatata
       61 aaagcttaga ctattttggc attgacgaaa tgctgacaga tgaagagaga tccatcagag
      121 atacagtaag agattttgta aacgaagaag tacttccaat aattgaagag tacaatcaaa
      181 aaatggattt tcccaaacag ttaattccga aaatggcgga acttgggatt tttggtccta
      241 ctcttcctgt agagtacggc ggcatgggaa ttaacaatgt tgcttacggt cttatcatgc
      301 aggagctgga aagaggtgac agcggtgtaa gaagttttgc ttccgtgcag tcggcacttg
      361 tgatgtatcc gatctataca ttcggaagtg aagagcagag aaaaaaatat ttacctaaac
      421 ttgcaagtgc tgaaatgatc ggatgtttcg gactgactga acctgactac ggaagtaatc
      481 cgggcggaat gatcacgaaa gcagagaaag ttgacggagg atacattctt aacggcgcaa
      541 agatgtggat cacaaatggg actgtcgctg acgtagcagt tgtctgggca aagcttgacg
      601 ggaaagtaaa aggatttctt gttgataaag gaacgaaagg attttcagct ccggagatga
      661 aaggaaaact ttctctcaga gcttccatta catcagagtt ggtttttgaa gactgtttta
      721 ttcccgatga aaatttactt cctgaaagcg gcggattgaa gtcccctctt atgtgtctga
      781 ctcaggcaag atatggaatt gcatggggcg tgattggact ggcaaatgaa tgttacagta
      841 cagtattaaa ttattcactt tcgagaattc agtttgataa gccgcttgct gcatttcaga
      901 tcactcagga aaaacttgca tacatggtat cggaaattac caaagcacag ctgctttgtt
      961 ttcagcttgc aaagttaaaa gatgcaggta agatgcgtcc tcagcaggtt tcccttgcaa
     1021 agagaaataa ctgcgagata gcaaagatca ttgcttcgat gtccagagag atgctcggtg
     1081 ctaacggaat tcttgatgaa tatcctatca tgagacatca gaataatatt gaatctgtaa
     1141 agacttatga aggtacacat gaaatgcata cacttataat tggagaagat gttacaggaa
     1201 tagctgctta taaataattt gttataaaat ctttttcctt gtaactgcag agacatgtgt
     1261 tcttcggact ctgcggaaac tgatttaaac aatttaaaca ggtaaattag aattaaattt
     1321 tcagaaatat tcattagaaa tgaaaacatt tgacatacca atattttaca gaagtccggt
     1381 aatctcaaag attaaaaatt cacggaagct gaatgatccc aggaaaaaag attactcccc
     1441 tactgaatta gattttggac cggttgtatt ttatattgcc agacatttcg gattctgtta
     1501 tggtgtggaa aatgcaattg aaatttcata caaagcaatt gaagaaaatc cgggtaagag
     1561 aattttctta cttagtgaaa tgattcataa ctctgacgtg aacagtgatc ttatcgaaag
     1621 aggagttaaa tttataatgg atacttcggg tatgcagtta atagattgga atgaactcca
     1681 ttcagaagat atagtgatta taccagcatt cgggacgaca cttgagattg aaagaaagct
     1741 caatgaactt aacatagatc ctttaaaata taataccaca tgtccgtttg ttgaaaaagt
     1801 ttggaacaga gctgactcac ttgggaaaac aggattcact atcatagttc acggtaaata
     1861 caaacatgaa gaaacacgcg caacattttc acacagtaaa gaatccgctc caacgcttat
     1921 catccgagat attactgaag ctgaaattct tagtgaattc ataaccggca aaagagatat
     1981 aaaagagttt tatgaaatat ttgcgaacag atattcagaa ggatttgatc ctgtaaaaga
     2041 tttgaattct atcggagttg tgaatcagac tactatgctt gcaactgaaa cacaggcaat
     2101 cgcagattat ctaaataaaa ttatgtcgga gaaattcggc acagaaaaat tttccgaaca
     2161 tttttctgac acacgggata cactttgtta tgctactcat gataatcaga aagcgactta
     2221 cggactgctg gaaaaaaatg ctgacatcgc aatagttgtc ggcggataca acagctcaaa
     2281 tacatcacac atttatgaat tatgtgaaga gaaacttccg acatatttta tttcatcagc
     2341 agataaaatt ttatcaaaag aattgatttc acattttgat tttcagaatc agaaggaaat
     2401 tttcactgac aattttttac ctgtaaaaga caaagtgaaa attatcctta cgagcggtgc
     2461 ttcgtgtccg gattcgatcg ttgatgaagt tctacaaaaa ttactttcgt attttaacag
     2521 cactaaaaaa atcgatttgg tactggaaga attttctatg aataaaaatt aattatacta
     2581 tttccaactg atcttacttt atgagtttat ttcgtgtaat actcgctatc attcttcctc
     2641 ctcttgcagt tattgacaaa ggctgcggtt cagttgttat agttgcaata cttacactta
     2701 tcggttgggt tccgggggta attgctgctt taattatttt aaataaaaat gaaagttagt
     2761 atttttttat tcttatccgt ccaaaacgtt ttagtataaa attaaatgag agtaagtttt
     2821 ttgttttaaa cattaaactg cattaagaaa tttattttaa ctatttaatt gaattttttt
     2881 aattttttag ttgcaaaagt tttaactttt
//
LOCUS       DAORVQ010000311         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31152_length_2903_cov_6.469452, whole genome shotgun sequence.
ACCESSION   DAORVQ010000311 DAORVQ010000000
VERSION     DAORVQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31152_length_2903_cov_6.469452"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2141
                     /locus_tag="PLX80_14450"
     CDS             <1..2141
                     /locus_tag="PLX80_14450"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY22040.1"
                     /translation="KINIMNKLFTTFLIIISAISMNLNAQSVWQWQQPQPTGNYLYTV
                     DFINENTGFAGGKLGTFMKTTNGGINWEASTINPDFEILGIDFYDAELGFAVGYLNGS
                     IFRTTNGGENWSNVFTSGVVALWDIYFPSRNIGYAAGLNGRIYKSTNSGLNWIQQNSL
                     TGSNLFSIDFIDTLNGVAGGTNIITKTTDGGQTWINQNIVFANVTTIVTTADYIDENN
                     IICLAQGDDRIYKTSNNGINWNAIIINRTDNDFQRTISFINPDTGIMVTDYGRIMQTT
                     NGGVSWNTDSSFVTPGYDNLILRFSDFESNNVAYVSGSGGRVIKSTNGGLNWFTTTGG
                     RYNYNSNYFIDQNTGFSVGNEGKILKTTNAGVNWVEKQSNTVQNLNEVQFVRNDIGYI
                     IGDTGVVLKSTDGGEIWHNLNLGVTSNFNGLFFTDINSGFICGNHSKIMRTTDGGTNW
                     ISQNSGVSANAVLGKLFFVNSDTGFMSGSFYLRTTNSGINWISDGNSGGLDVFFTDYY
                     NGYRPGGSGIILKSTDGGINWIVQSGNVLANINSIFLLNKNNGFAVGNDGAVTKTTNG
                     GINWIPQAKLTANSLNSVFFTDADNGYITGDFGTLIKTTNGGLTFITQNSVSYPKDFT
                     LYQNYPNPFNSGTVIKYNLRTSGFVELKLYDITGKEIQKLVKEYQSAGSYNVSFNSSN
                     YGNNIHSGIFLYSLYIDKKIINTKKLILIK"
     gene            2152..>2903
                     /locus_tag="PLX80_14455"
     CDS             2152..>2903
                     /locus_tag="PLX80_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22041.1"
                     /translation="MKTIHFLIFMSLFLNSGNAHLTNDYPFSTHLDSINNLYVTGNKY
                     NLASNSIDILFEKYNSGGDRLIYELILNSFGNDRGMDIITDNFGNSFITGFIYNNIYE
                     SNDIIALMFDPDGNLVWVKKFENPGDDKGFGIDIVSDIYGQATEIYISGYSSNELSNK
                     NFFVSRLNLLGDVVWQRELNLSDKYSIATDIFVDAGYVYVSGYSYQGPAYGDDITLVT
                     LDKASGTIEQGDILFHNIAGSHERPTAFTLVN"
BASE COUNT         1032 a          369 c          554 g          948 t
ORIGIN      
        1 ctaaaataaa tattatgaat aaactcttta caacatttct aataatcatc tcagcaatct
       61 caatgaattt aaatgcccag tccgtctggc agtggcagca gccgcagcct acaggaaatt
      121 atctttatac ggttgacttc ataaatgaaa acacaggatt tgccggcgga aaattaggta
      181 catttatgaa aacaactaat ggcggtatca actgggaagc aagtactatt aatcctgatt
      241 ttgaaattct ggggattgat ttttatgatg ctgaactagg atttgctgta ggttatttaa
      301 atggttcgat attcagaaca actaacggag gagaaaattg gtcaaatgtt tttacatcag
      361 gtgttgtagc tctttgggat atttattttc catccagaaa tatcggttat gctgcaggac
      421 tgaacggaag aatttataaa tctacaaatt caggtttgaa ctggattcag caaaatagtc
      481 tgaccggttc aaatttattt tcaattgatt ttattgatac tttaaacggt gttgccggag
      541 gaactaacat tattactaag actactgacg gcggtcagac atggataaat cagaatattg
      601 tttttgcgaa tgttacaaca atagtcacaa cagcagatta tatagatgaa aataatatta
      661 tttgtctggc gcagggcgat gacagaattt ataaaacttc caataatggg atcaactgga
      721 atgcaattat tattaacaga acagacaacg atttccaaag gacaatttca tttattaatc
      781 cggatacggg aatcatggtg acagattatg gaagaattat gcaaactaca aatggcggag
      841 taagctggaa cacagattca tcatttgtaa cgccaggtta tgataactta atattaaggt
      901 ttagtgattt tgaaagtaat aatgtagctt atgtcagtgg ttccggaggc agggtaataa
      961 aatctacaaa cggcggttta aactggttta caacaaccgg cggaagatat aattataatt
     1021 caaattattt tattgatcag aatacaggtt tttctgttgg taatgaagga aaaattttga
     1081 agactacaaa tgcgggagtt aactgggttg agaagcaaag taatactgtt cagaatttga
     1141 atgaggtgca gtttgtgaga aatgacattg gatatataat tggagatact ggtgtagttt
     1201 taaaaagtac agacggagga gaaatatggc ataatttaaa tttaggtgtg acgagtaatt
     1261 ttaatggttt attttttact gatattaatt ccggatttat ctgcggaaat cattcaaaga
     1321 ttatgaggac aacagatgga ggtacaaatt ggatttcaca aaatagtgga gttagtgcaa
     1381 atgcggtatt gggaaaattg ttttttgtta attctgatac tggatttatg tccggtagtt
     1441 tttatttacg aacaacaaac tcaggaataa actggattag tgacggaaac agcggaggat
     1501 tggatgtatt ttttacagat tattataatg gatatagacc aggaggttcg ggaattatat
     1561 tgaaatctac ggatggtgga attaattgga ttgtacaaag tggaaatgtt ctagctaata
     1621 taaattctat tttcttatta aataaaaata atggatttgc agttggcaat gatggagctg
     1681 ttactaaaac tacaaatggc ggaataaatt ggattcctca ggcaaaactt acagctaata
     1741 gtttaaattc cgtattcttt acagatgcag ataatggcta tataaccgga gactttggga
     1801 cacttataaa aacaacaaat ggcggactga catttattac tcaaaattct gtgtcctatc
     1861 ctaaagattt tactctctat caaaattatc caaatccatt taattcagga actgtaataa
     1921 aatataatct aagaacttct ggttttgtag aattgaaatt atatgatatt acaggtaaag
     1981 aaattcaaaa attagttaag gaatatcaaa gtgcaggaag ttataatgta tcatttaatt
     2041 catcaaatta tggaaataat attcattcgg gaatatttct ttattctttg tatatagata
     2101 aaaagattat aaatacaaaa aaattaatat taattaaata aaaacaaaat tatgaaaaca
     2161 atacactttt taatttttat gtccttattc ttaaattcag gtaatgcaca tttaacaaat
     2221 gattatccct ttagtactca tctagattca ataaataatt tgtatgttac cggaaataaa
     2281 tataatctag catcaaattc aatagacata ctttttgaaa aatacaactc tggaggggat
     2341 agattaattt atgaattaat tttaaatagt tttggaaatg atagaggaat ggatataatt
     2401 acagataatt ttggtaattc ttttattaca ggctttattt ataataacat atatgaaagt
     2461 aatgatataa ttgctttaat gtttgatcca gatggaaatc tagtttgggt taagaaattt
     2521 gaaaaccccg gggatgataa aggttttggt attgatattg tttcagatat atatggacaa
     2581 gcaacagaaa tttacatatc aggatattca tcaaatgaat tgtcaaataa gaatttcttt
     2641 gtaagtagat taaatttatt aggagatgta gtctggcagc gcgaactaaa cctctccgat
     2701 aaatacagca tcgcaactga catatttgtc gatgccgggt atgtttatgt ttcgggttat
     2761 agttatcagg gtccagctta cggagatgat attacattag tgactttgga taaggccagc
     2821 gggaccattg agcagggaga tattttgttt cataacattg ccggcagcca cgaaagacct
     2881 acagctttta ctttagtaaa tag
//
LOCUS       DAORVQ010000312         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31204_length_2900_cov_5.914236, whole genome shotgun sequence.
ACCESSION   DAORVQ010000312 DAORVQ010000000
VERSION     DAORVQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31204_length_2900_cov_5.914236"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..2767
                     /locus_tag="PLX80_14460"
     CDS             197..2767
                     /locus_tag="PLX80_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22042.1"
                     /translation="MKKFILSSLFLSCLFLVPDFSYSSILYKSNSSGNWNSISSWLIS
                     INSGSSWSAPVNAPDTYNCDEIRILSTHNVTVTSNTNAGILVIDNGGILTINSGIELE
                     IINKSGIDLNLFNGGTIAGSGTISTASSGVTDLNFQGYSVFSVKLNVKSSAGITNIYG
                     STFPYKPILRNELTVDAGGVIQCNSLQGFETRSPVTNNGTISGTNTMSAKGNIINNSV
                     ININNLSIDSNLTISGTGAVSSPSLTIGIIKTLTLGNDMTFNSPNIYCNGTILLFNGS
                     GRNLTLGANCNLYVYSFGNLSNGSIFLNGGNIDIQNGTFSSILNAVSGTTLILNSLTQ
                     STANIGTVNVYPNANLNVQSGDTMKVTGTMTNEGNVGGSGWMHYNAAFMNNYNIISVS
                     NLNFAQGVFTIIQGDTGKITSNVMNIKNNTTLYLSNHSLNNVNVNSGSTLRFDYFFPI
                     YSTVKFLSSDPIVNSGTINQTKGIIEYAGSAGQVISAMTYRSLKINNPGGTQAGNNFN
                     VTDSLKIVSGDLDLNGKEITLNTSAYLSETNGNNVTGYTGSIKTTRTISNPSNYSFGG
                     LGLTVTSSANLGSTLVKRTHSAAVINGYNSVIRNFEITPSNNSGLNATLNFYYDESEL
                     NGNSGTNLALYKSTNSGVSYSFQGGNADDGNNKVTKTGIGSFSRWSLSRAVYPASIKI
                     ITEGLYISDKLNKRDTAKVYLRNISMPYAIIDSAVSEIDSINFTGIFDFKNAPAGNYF
                     YQIKHKNSIETWSKTGGENYLGGISNSYDFTNSVNKAYGNNLVKVNNSPQRFAVYSGD
                     IDQDGAIDVSDGSITENDAYAFISGNVITDLNGDGIVDIGDVVFVDNNASNFIIVLKP
                     "
BASE COUNT         1008 a          458 c          525 g          909 t
ORIGIN      
        1 cttatcagag taatgtaaga tggaaggcaa actactttga atatattttt tctttgaaga
       61 tagtatcgaa aattgttact gaaaatctta aatggattct aatgaaataa taaattaaat
      121 tataatcccc cgaattttga cataggagaa caggcttttc aatatattat aattttaaaa
      181 aatataaaaa gtctttatga aaaaatttat tctttcttcg ttatttttat catgtttatt
      241 tttggttcct gatttttctt attcctccat cctttacaaa agtaattcat ccggcaactg
      301 gaattctatt tcttcttggc tcatctctat aaacagcggc tcgagctgga gcgcaccggt
      361 taacgctcct gatacttata actgtgatga gataaggatc ctcagcactc ataatgtaac
      421 ggtcacttca aatacaaatg cgggaatttt agttattgat aacggaggta tactgaccat
      481 taattccggc attgaacttg agataataaa caaatcagga attgatctga atttattcaa
      541 tggaggtaca attgccggat ccggtacaat atccacggca tcctccggtg tgactgatct
      601 gaactttcag ggatattcag tattcagcgt aaaactcaat gtgaaatctt cagcaggaat
      661 aacaaatatt tacggatcga ctttccctta taaacctata ctaagaaatg aattaaccgt
      721 agatgccggc ggagtaatcc agtgcaacag tttacaggga tttgaaacaa gatctcctgt
      781 aaccaataac ggaacgatca gcggtactaa taccatgtct gccaaaggaa atattatcaa
      841 taactctgtt atcaatatta ataatctttc catcgattca aaccttacaa tctcaggaac
      901 cggtgctgta agttcaccaa gccttacaat cggtatcatc aaaactctga cacttggaaa
      961 tgacatgaca tttaattcac caaatattta ttgcaacggt actatattat tattcaacgg
     1021 cagcggaaga aatcttacgt taggtgctaa ctgcaattta tatgtttaca gttttggaaa
     1081 tttaagcaac ggtagtatat ttttgaacgg aggaaatatc gatattcaaa atggaacatt
     1141 ttcatctatt ttgaatgcag tatcaggcac aacattaatt ttaaattcat taactcagag
     1201 tactgcaaat atcggtactg tgaatgtata tccgaatgcg aatttgaatg tgcaatcagg
     1261 ggatacaatg aaagtcaccg gtacaatgac aaatgaaggc aatgtcggag gcagcggctg
     1321 gatgcattac aatgctgctt ttatgaataa ttacaatata atatcagtaa gtaatttaaa
     1381 ttttgcacag ggagttttta caataattca gggagatacc ggaaagatca cttcaaatgt
     1441 aatgaatatt aaaaataata ctactctgta tctgagtaat cattcgctga ataatgtgaa
     1501 cgttaattcg ggtagtacat taaggtttga ttattttttc ccgatttatt caacagttaa
     1561 attcctttcc tcagatccaa ttgtaaacag cggtactatt aatcagacga aaggaataat
     1621 agaatatgca ggatctgccg gtcaggtcat ttcagctatg acatacagat cattaaagat
     1681 caataatccc ggtgggactc aggcgggtaa taattttaat gtaacggatt cactaaaaat
     1741 tgtatccgga gatctggatt tgaacggaaa agaaattact cttaatacct ctgcatattt
     1801 atcagaaaca aatggaaata atgtgacagg atacactggt tcgatcaaaa ctacaagaac
     1861 aatttcaaat ccttccaatt acagtttcgg gggtttagga cttacagtta ccagctctgc
     1921 aaatttagga tcgaccttag taaaaaggac tcacagtgct gcagtaataa atggatataa
     1981 cagtgtcatc agaaattttg aaattactcc ttcaaataat tcaggtctga atgcgacatt
     2041 gaatttttat tatgatgaat cagaactgaa tggaaacagc ggaacaaatc ttgcattgta
     2101 taaatcaaca aacagcggtg tcagctattc atttcagggg ggaaacgcag atgatggaaa
     2161 taataaagtt acgaagacag ggattggcag cttttcaaga tggtcattat ccagagcagt
     2221 gtatccggct agtataaaaa taattacaga aggattatat atttccgata aactaaacaa
     2281 acgtgatact gccaaagtct atttaagaaa tatttcaatg ccttatgcaa taattgattc
     2341 agcagtatct gaaattgact ctataaattt tacaggaata tttgatttta aaaatgcacc
     2401 ggcaggaaat tatttttatc agataaaaca taaaaattca attgagacct ggagtaaaac
     2461 cggaggtgaa aattatctgg gaggaatttc caacagttat gattttacaa attctgtgaa
     2521 caaagcatat ggaaataatc ttgttaaggt aaataattct cctcagagat ttgctgttta
     2581 cagtggagat atagatcagg acggcgctat tgatgtgtcc gatggaagta ttacagagaa
     2641 tgatgcttat gcttttattt caggaaatgt aattacagat ctgaacggtg acggaattgt
     2701 agatattgga gatgttgttt ttgttgacaa taatgcttca aattttataa tagttttaaa
     2761 accttaatca gatgaattat tattgttgta attcgaaatt ctgcagatca gtaaaatctg
     2821 tgaagcaaga gatggtaata taagatcata tctactttga aagattctca agtttgatcc
     2881 tttcctgagg agttgtcatt
//
LOCUS       DAORVQ010000313         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31205_length_2900_cov_5.826011, whole genome shotgun sequence.
ACCESSION   DAORVQ010000313 DAORVQ010000000
VERSION     DAORVQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31205_length_2900_cov_5.826011"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLX80_14465"
     CDS             <1..371
                     /locus_tag="PLX80_14465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22043.1"
                     /translation="GNYSYRLKQVDFNGNFEYFNLSSEILVGVPEAYKLSQNYPNPFN
                     PSTKIDYDLPYDGKVSILLYDISGREVANLVNEVKTAGYYTATFNGANLSSGMYFYRI
                     NATGTGNNNFVSTKKMVLIK"
     gene            610..>2900
                     /locus_tag="PLX80_14470"
     CDS             610..>2900
                     /locus_tag="PLX80_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY22044.1"
                     /translation="MKKIYNIAFLPIFLLSFILLIPKESKANLYWNQAVSFAGSTDSY
                     IASRDDADLDITGDFTIEAWVNPTNSLSPTFQIIFQKRQASAAGYTLYLNNGRVTIRT
                     LSTTRLVGKTVIPSNEWTHIAGTYDLSSNTFKVFVNGDLDTSVVIAGAAPTANNDSLL
                     IGKGSNDPFAGMMDEIRIWNTDNSSNINKYHRTSLAGSSGKYDGLVYSLTFQDDDDFG
                     NVFSLNDFTLKNIAAKNNGVSAVDLSNRPSNTIHPNDCLDLVGNNSYLSAPDSPIISP
                     VTGITLSAWIYPRSNLNSILIHKGPVAGGAGTDYRMALLNGNLFTGINGTFFTPGSVA
                     AVSENKWSYVVFTYDGSSGNYKFYVNGKNVAGGIQNLGNINNGTDSLYIGGTLSLNDF
                     DGYIDEVSISPEAKTEEFIGDYMFRSRNLGNVSSQADVVYNFDGYSYSNSGDLATTGK
                     QLFFRNAAGFAHNGGAGNAVESPLIGNDNSEFQEGFHLKSSDRRIPETGTSGNMISDT
                     LEILQGGTVNNIKLFIGLNHTYEGDLQISLTNPAGNTVTVFDNMDYLGSGENLVTIFN
                     DLADSSLLDFRYVNLSPQVKPLNNMISVFGLSGIKGKWILKINDQITADSGRLSGWGI
                     QFNNETAKPKKLNLTAFIQGFYNLGTNQMIRDTMKFYLRDQLSPHDIVDSAKAFASVS
                     GFSDVNFSNAVAGRPYYLTVNHRNLIETWSSSTVSFDPLTYQLEYDFTKNAASAYGSN
                     LIPVDLAPLNYACYNGDVNQDQT"
BASE COUNT         1006 a          466 c          544 g          884 t
ORIGIN      
        1 caggaaacta cagctacaga ttaaagcaag ttgatttcaa cggtaatttt gaatatttca
       61 atctttcaag cgaaatctta gtaggtgtac ctgaagctta caaactttca caaaactatc
      121 caaatccttt caatccatca acaaagattg attatgatct tccttacgac ggaaaagtca
      181 gtatcttact ttatgacatc tccggtagag aagttgcaaa cttagtaaac gaagtgaaaa
      241 cagctggtta ttacacagct actttcaacg gtgctaactt atcaagtgga atgtacttct
      301 acagaatcaa tgcaacagga accggtaaca acaactttgt atcaacaaag aaaatggttc
      361 ttatcaagta atatagttct tagaaacaat ttaaaagggt tggcttagcc agcccttttt
      421 ttttatactt tttatacttt tgcaataaag gaagcaatat aaatataaag atgattcaaa
      481 ttaccaattc agaatacatg aaatcgtttt aaatttttca taaaggaaga aagctttagt
      541 aagttaagtt tacctgaaag aatgtttgcg gcaagacatt tttaaactaa tttaacgaaa
      601 ggtgttgaaa tgaaaaaaat atacaacata gcgtttctgc caatattttt actaagcttt
      661 atcctcttaa taccaaaaga atcaaaagca aatctttact ggaatcaggc agtttctttt
      721 gcaggatcta ccgatagtta cattgcctca cgagatgatg ctgatctgga cattacagga
      781 gattttacta tagaagcctg ggtaaatcca acaaattcat taagtccgac atttcagata
      841 attttccaaa agagacaggc atcagctgca ggttatactc tttatttaaa taatggaaga
      901 gtaacaatca ggacattaag tactacacgt cttgtaggaa aaactgtgat cccgagtaat
      961 gaatggacac atattgcagg aacctatgat ctaagctcaa atacatttaa agtatttgta
     1021 aatggagatt tagacacaag tgtagtgata gccggagcag ctccaacagc aaataatgat
     1081 tcattattaa taggaaaggg atctaatgat ccatttgccg gaatgatgga cgaaataagg
     1141 atctggaata cagataacag ttcaaatata aataaatacc acagaacaag tcttgcagga
     1201 agcagcggaa aatatgacgg actcgtttat tctcttacat ttcaggatga tgatgatttc
     1261 ggaaatgtgt ttagcctgaa tgattttaca ttaaaaaaca ttgcagctaa aaacaacgga
     1321 gttagtgcag tagatctcag taacagacct tccaatacta ttcatccaaa tgattgttta
     1381 gatctcgttg gaaataacag ctatctgtca gcaccggact ctccgataat cagtccggtt
     1441 acaggtatta cattaagcgc atggatctat ccaagatcaa atttaaattc tatattaata
     1501 cataaaggac cggttgcagg cggagccgga accgattatc gtatggcatt attgaacgga
     1561 aatttattta caggtattaa cggaacattt tttacccctg gaagtgtggc tgcagtttct
     1621 gaaaacaaat ggtcttatgt tgtatttact tatgatggaa gtagcggtaa ttataaattt
     1681 tacgtaaacg gtaagaatgt tgccggtgga attcaaaatc ttggtaatat aaataatggt
     1741 acagactcat tatatattgg aggtacatta tctttaaatg attttgacgg atatattgat
     1801 gaagtatcaa tatcgccgga agctaaaact gaggaattta ttggtgacta tatgttcaga
     1861 tccagaaatc taggaaatgt gtcttcacag gctgatgttg tttacaattt tgacggatat
     1921 tcttattcaa attcaggaga tcttgctact accggaaagc aattgttttt tagaaacgct
     1981 gcaggatttg ctcataacgg cggagctgga aacgctgtag aatcaccttt aattggaaat
     2041 gataattctg aatttcagga aggatttcac ttaaagtctt ctgatcgcag gatacctgaa
     2101 acaggaacaa gcggaaatat gatcagtgac actttggaaa tattgcaggg aggaacagta
     2161 aataatataa aattatttat aggacttaat catacatatg aaggagatct tcagatctca
     2221 ttaaccaatc ctgcaggtaa cactgtaaca gtttttgata atatggatta tcttggttca
     2281 ggtgaaaacc ttgtgactat atttaatgat ctggcagaca gctctttatt agatttcaga
     2341 tatgtaaatc tgtcaccaca ggtcaaacca ttaaataata tgatatcagt gtttggtctg
     2401 tcggggatta aaggaaaatg gatattaaaa ataaatgatc agattactgc tgattcaggc
     2461 agattgtccg gctggggaat tcagtttaat aatgagactg caaagcctaa aaagcttaat
     2521 ctgactgcat tcattcaggg attttataat ttaggaacaa atcagatgat aagagatact
     2581 atgaaatttt atttaagaga tcaattatct cctcacgata tagtcgattc agccaaagca
     2641 tttgcatctg tttctggatt ttcagatgtt aattttagta atgcagttgc cggcagacct
     2701 tattacctga cagtaaatca cagaaatctt atagagacat ggagctcttc gactgtaagt
     2761 tttgacccgc ttacatatca gttagaatat gatttcacaa aaaatgcggc aagtgcttat
     2821 gggtctaatc tgataccggt tgatcttgca ccgcttaatt atgcctgcta taacggagat
     2881 gtaaatcagg atcagacaat
//
LOCUS       DAORVQ010000314         2889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31431_length_2889_cov_5.857092, whole genome shotgun sequence.
ACCESSION   DAORVQ010000314 DAORVQ010000000
VERSION     DAORVQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31431_length_2889_cov_5.857092"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..353
                     /locus_tag="PLX80_14475"
     tRNA            280..353
                     /locus_tag="PLX80_14475"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:314..316,aa:Pro,seq:cgg)
     gene            413..1990
                     /locus_tag="PLX80_14480"
     CDS             413..1990
                     /locus_tag="PLX80_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HQY22045.1"
                     /translation="MKKSILSFLFLVLSISGISFSQNTDSQSETNSQKYKDITILHWN
                     DFHARNQPYKVNKKDKDSGEETSYIVGGTSGMLGYINKFRNGNTLLVNGGDDFQGSPI
                     STITRGKSQIELLNLYGLDAFVIGNHEFDYGQYALDSVLMLAEFDHLCANAFLTTMDK
                     TVADRYKIKEVNGVKIGIIGLTALDLMTLVLPKNVSDIQMLNTDSVVTAGINYMKEND
                     CDIIMLLTHIGVDNDKMLAEKFYKDIDLIVGGHSHTPLFKPVIQNDVVIVQAGSYGRY
                     LGQMELVVDRENDTLVEYKTWLTETVFDSTINDKAAGEKVDKMLEEIEGYLSTVIGQL
                     ENDWVRGNTNESNLGQWETDAVRTATGTDIAFLNSGGIRKDLYKGDITVGDMWEINPF
                     GNTIVKINVTGKMLKEMFENFIFMSLDTSGERSSGDKIIISGLYIEFDPKDGTKGNYI
                     VSMQVNGKEVSDEMIYSFSTNNFVGSQFPKYFGEVSGEINVEDTGIVDRDLFIEQVKR
                     EGIINSVADIRMKDISK"
     gene            2011..2295
                     /locus_tag="PLX80_14485"
     CDS             2011..2295
                     /locus_tag="PLX80_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HQY22046.1"
                     /translation="MSEERLVNCIKMEQELPGLDKPPIDGELGQKIYDSVSKQGFKLF
                     LDHFKMVVNEYRLDLTSPATDKVFEDQMREFFFGDGIKLPDEFVPPDESK"
     gene            2408..>2889
                     /locus_tag="PLX80_14490"
     CDS             2408..>2889
                     /locus_tag="PLX80_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22047.1"
                     /translation="MNNLDTTHTLDTIESFDEYILMPRVQAYKLFIAQKNLKIFRVVL
                     NIFIILCSIVCIVNFANQEYLNLSLVITLVNLFLLIVARYFYKKYFGIKNIRRSIFAF
                     LIIQLLLFISIEVFYPSESENDLQDSESSSEELKKELSKDDINVSVSTGDDDNFLSYI
                     "
BASE COUNT          996 a          442 c          571 g          880 t
ORIGIN      
        1 tttggttctt tggagcctgc ccccttgtgc ttccaaaggg ggatcgttca aaaaaagaac
       61 attagaaaat acaaatatct aattttactg ataaacgttt tgggcggatg tgctgaaaat
      121 ccaattgatt taaaaattat aaatcaatta gaattctaaa atatattagt ttgatttgtt
      181 caagaaataa actcagctca gattacatga taatttcaca tttaaatcaa actatatatt
      241 atttatattt gcacatacaa aaacctgatc gcaggatttc gggatgtagc gtagctcggt
      301 agcgcgcctc gttcgggacg aggaggtcgc tggttcaaat ccagtcatcc cgactcgttg
      361 tatccgccgg aaaaattttc cgtcagtaat ttaattccag aatcaattta ttatgaagaa
      421 atcaatttta agttttttat ttctagtcct gtcaatatca ggtatttcct tttcacagaa
      481 tactgattct caaagcgaaa caaattcaca aaaatacaaa gatatcacca ttttacactg
      541 gaacgatttt catgcaagga atcagccata taaagtgaat aaaaaagata aagactccgg
      601 ggaagaaact tcctatatag tgggcggcac ttcgggtatg cttggatata ttaataaatt
      661 cagaaacggc aacacgctgc ttgtcaacgg cggagacgat tttcagggct ctccgatctc
      721 aactattacc agaggtaaat ctcagatcga acttctgaac ctttacggtc tcgatgcatt
      781 cgttatcggt aatcatgaat ttgactacgg acagtatgcg ctcgattcgg ttttaatgct
      841 cgcggaattt gatcaccttt gcgcaaatgc ttttcttaca acaatggaca aaacggtagc
      901 agacagatat aagatcaaag aggtcaacgg agtaaagatc gggatcattg gtctgacagc
      961 tctcgatctc atgacacttg tcttacccaa aaatgtcagc gacattcaaa tgctgaatac
     1021 ggattccgta gtaaccgcag gaataaatta catgaaggaa aatgactgcg atattataat
     1081 gctgcttact catattggtg tggacaatga taaaatgctt gcggaaaaat tttataaaga
     1141 tattgattta atagtcggcg gacattctca tactccgctt tttaaaccgg ttatacaaaa
     1201 cgatgttgtg atagtgcagg caggatctta tggcagatat ctcgggcaga tggagttagt
     1261 tgttgacaga gaaaatgata ctctagttga atataaaaca tggctgactg aaacagtttt
     1321 tgattctact attaatgaca aagccgcagg tgagaaagtt gataagatgc ttgaggaaat
     1381 tgaaggttat ctttcaacag taattggtca attagaaaat gactgggtac gcggaaatac
     1441 aaacgaaagt aatcttggtc aatgggaaac cgatgccgtg agaacagcta cgggaactga
     1501 cattgcattt ttgaattcag gcggaataag aaaagatcta tataaaggag atattactgt
     1561 cggtgatatg tgggaaataa atccttttgg aaatacgatc gtgaagatca atgtcaccgg
     1621 taaaatgctg aaggaaatgt ttgagaattt tattttcatg agtcttgata cttcgggaga
     1681 aagaagttcg ggtgataaaa ttatcatatc aggactgtac attgagtttg atcccaaaga
     1741 cggtacgaaa ggaaattaca tagttagtat gcaggtgaat ggtaaagaag tttctgatga
     1801 aatgatctat agtttttcga caaataattt tgtcgggagt cagttcccaa aatatttcgg
     1861 agaagtatca ggagagatta acgtcgagga tacgggaata gttgacaggg atcttttcat
     1921 tgagcaggta aaacgcgagg ggataattaa ttccgtggca gatattagaa tgaaagatat
     1981 ttcaaaataa aaatttaaat aaaaataaaa atgtctgaag aaagattagt aaattgtata
     2041 aaaatggaac aggagcttcc cggattagac aagccaccga tagacggaga gctcggacag
     2101 aagatatatg acagtgtgtc gaagcaggga tttaaattgt ttctcgatca tttcaaaatg
     2161 gtagtaaatg aatacagatt agacctgacc tcaccggcta cggataaagt ttttgaagat
     2221 cagatgaggg aattcttttt tggagacggt ataaagcttc ctgatgaatt cgtcccgccg
     2281 gatgagtcta aataattata ttagagcaac aattcattta tatgcccgtt tactttttgt
     2341 ctaatcatgc cttaaaatac tattataaca gattgccaat aattaaactg caaaaaaaaa
     2401 taaattcttg aacaatttag atactacaca tactcttgat acaattgaat catttgatga
     2461 atacattctt atgccgagag tacaagcata taaattattc atagctcaga aaaatctaaa
     2521 aattttcaga gtagtattga atatttttat aattctatgt tcaattgtct gtattgttaa
     2581 ttttgcaaat caggaatatt taaacctatc attggtcatt acactggtca atctatttct
     2641 ccttatagtt gccagatatt tttataaaaa atattttgga ataaaaaata tccgcagaag
     2701 catatttgct tttctgataa tacagttgct gttatttatt tcaatagaag ttttctatcc
     2761 ttctgaaagc gagaatgatt tacaggatag tgaaagtagc agtgaagagc ttaaaaagga
     2821 actttctaaa gatgatataa atgtttcagt aagtacaggt gatgatgata attttttatc
     2881 ctatattat
//
LOCUS       DAORVQ010000315         2869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31805_length_2869_cov_3.052950, whole genome shotgun sequence.
ACCESSION   DAORVQ010000315 DAORVQ010000000
VERSION     DAORVQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31805_length_2869_cov_3.052950"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..179)
                     /locus_tag="PLX80_14495"
     tRNA            complement(93..179)
                     /locus_tag="PLX80_14495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(143..145),aa:Ser,seq:cga)
     gene            complement(322..409)
                     /locus_tag="PLX80_14500"
     tRNA            complement(322..409)
                     /locus_tag="PLX80_14500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(373..375),aa:Ser,seq:gga)
     gene            complement(508..912)
                     /locus_tag="PLX80_14505"
     CDS             complement(508..912)
                     /locus_tag="PLX80_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQY22048.1"
                     /translation="MNYWLLKTEPTVYSYDDLVKDKKAVWDGVGHNTALINIRNARKG
                     DLAFIYHTGSERQVIGISEIISDPYKDPKTDNPKMAVFDIKPVSKLANPVTLAQMKED
                     KIFSEFRLVKESRLSVVSVPENLWKEIIKMGK"
     gene            complement(930..>2869)
                     /locus_tag="PLX80_14510"
     CDS             complement(930..>2869)
                     /locus_tag="PLX80_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQY22049.1"
                     /translation="IGFTRNYSIYDSDESVNQVKQLMLSNSMPIDNPTPKSIHSFISN
                     LKNKLILPLEFSGMAKTPFDRKVEIIYDQYQKTLLKNNAMDFDDLLIKPIELFKRYPE
                     ILEKYQERFKFILVDEYQDTNKAQYMIVKMLSEKYRNISIVGDDAQSIYKWRGAEIQN
                     IFDFQEDYKDCKIFKLEQNYRSTQNILTLAGCVIKRNKKQIEKNLWTENSEGEKVHLI
                     ETMTDRDESVLICRYIFDEMQRKKLNFRDFVILYRTNAQSRTLEDGLRQNNIPYMIVG
                     GIRFYQRKEIKDILSYLKLIINPQDNESIIRVLNLKPGIGKTTIDKIIAIAESNQLQI
                     FDVLKKINETNEFSTRVRSLLTEILSYILKYQYLKEEMSLSELAGGVLDEMGILRTLR
                     LENTIEAEERINNIQELISAISEYSDTSDDATLENFLQQVSLVADIDSIDNQKNAVTL
                     MTIHSSKGLEFPVVFVTGLEEGLFPVSGALMYEEELEEERRLFYVAVTRAMEKLYLSY
                     ANQRYKFGTQSYQMKSRFIKEIPEEVINNNILFEGIKKFPGNRSHEVKKVSKPSLFGS
                     SLKYEPYKKKSNINSEVDDPLTDKFPDVQKGVVVFHNTFGKGTVLSIAGKGLDKKAEI
                     YFDEIGIKKIILKYAKMSIET"
BASE COUNT          864 a          547 c          400 g         1058 t
ORIGIN      
        1 ccgggagtta aaatttcata aaacaaagtg aaagtatttg aagatttaaa taaaataagg
       61 tgagttaaaa acagagtttc aacaaataat tgcggagaag gagggattcg aaccctcgat
      121 agagttgccc ctataacggt tttcgagacc gccgcattcg accactctgc cacctctccg
      181 gttttttaat ttcaagtttc aaattacagt ttacgagttt aattatagaa ttggatacct
      241 gattataatg taatacacat tatctgtaaa ttggcaataa tttgagtgtg aagtatcgta
      301 tgaatttcaa gaagttttct gcagagagtg agggattcga accctcgtag cgcttgtgac
      361 gctaacacac tttccaggcg tgctccttca accactcgga caactctcta attttgaata
      421 tgcaatataa atttttaaga actcaaattc tttgtaaaaa tttttgattc agtaaaaaat
      481 ttatttacag gagaaattaa atctaaatta ctttcccatt ttaatgatct ctttccaaag
      541 attttccggg actgaaacta ctgaaagcct gctctctttt accagtcgga attcagaaaa
      601 tattttatcc tctttcatct gcgctaaagt aaccggattt gcaagtttac ttacaggctt
      661 tatatcaaaa acagccattt taggattgtc tgtcttcgga tctttataag ggtcagaaat
      721 tatctcagaa attccaataa cctgtctttc acttccggtg tggtatataa aagcaaggtc
      781 ccctttccta gcatttctga tattgatcaa tgctgtatta tgaccaactc cgtcccatac
      841 ggctttttta tcttttacaa ggtcatcata actataaaca gttggttcgg tttttaataa
      901 ccagtaattc atttgacaag tattaagttt taagtttcta tgctcatttt tgcatatttc
      961 aaaataattt tttttattcc tatttcatca aaatagatct cagctttttt atcgagccct
     1021 tttccggcta ttgaaagtac agttccctta ccaaaagtat tatgaaaaac tactacccct
     1081 ttttgtacat ccggaaattt atcggttaat ggatcatcaa cttcgctatt gatgttactt
     1141 ttctttttgt atggttcata ttttaaagat gatccaaaaa gtgatggctt tgatactttc
     1201 ttaacttcat gacttcggtt tcccggaaat tttttgatcc cctcaaacaa tatattattg
     1261 ttaataactt cttccgggat ttctttaatg aatcttgatt tcatctgata tgactgggta
     1321 ccaaatttat atctttgatt tgcatatgaa agatataatt tttccattgc tcttgtaact
     1381 gcaacataaa acaatcttct ttcctcttca agctcttctt catacatcaa cgccccgctt
     1441 acaggaaaaa gaccttcttc aagtcctgta acaaatacaa ctggaaactc cagcccttta
     1501 gaagaatgta tcgtcattaa agtaacagca tttttctgat tatctatgct gtctatatct
     1561 gcaaccaatg aaacctgctg cagaaaattt tcaagagttg catcgtcact ggtatctgaa
     1621 tattcagata tcgctgaaat caattcctga atattattaa ttctttcctc tgcttcaata
     1681 gtgttttcga gtcttaaagt ccttaaaatt cccatttcat caagcactcc gcctgcaagt
     1741 tcggaaaggg acatttcttc ttttaaatac tgatacttaa gtatgtaact taaaatttct
     1801 gtcaataatg atcttactct cgtactgaat tcattcgttt cattaatttt ctttaataca
     1861 tcaaatattt gtaactgatt actttcagca attgcaataa ttttatctat agtggttttt
     1921 ccaattcccg gcttaagatt caaaactctt ataattgatt cattgtcctg aggatttatt
     1981 atgagcttta aataagaaag tatatccttt atttcctttc tctgataaaa tcttattcca
     2041 cctacaatca tataaggaat gttgttttgt cttaacccat cctcaagtgt tctcgactga
     2101 gcatttgtcc tgtataaaat tacaaaatcc ctgaagttga gcttttttct ctgcatctcg
     2161 tcaaaaatgt aacggcagat caatactgac tcatctctgt cagtcattgt ctctatcaaa
     2221 tgaacttttt caccttcgga attttcagtc cagagatttt tttcgatctg tttcttattt
     2281 ctttttataa cacatccggc aagagtaagt atattctgag tcgatctgta attttgttcg
     2341 agcttaaata tcttgcaatc cttatagtct tcctgaaaat caaaaatgtt ctggatctct
     2401 gctccgcgcc atttgtatat cgattgcgca tcatctccta ctatagaaat atttctgtat
     2461 ttctcagaaa gcatcttcac aatcatatac tgagccttat tagtatcctg atattcatca
     2521 actaaaataa atttaaatct ctcctggtat ttttccagaa tctcagggta tcttttgaat
     2581 aactctattg gcttaatcag cagatcatca aaatccattg cattattctt caataaagtc
     2641 ttttgatact gatcataaat aatctcaact tttctgtcaa aaggtgtttt cgccatacct
     2701 gaaaactcta acggcaatat taatttattt tttaaattac taataaaact gtgaatactc
     2761 tttggagttg gattatcaat tggcatggaa ttacttagca ttaactgctt tacctggttt
     2821 acagattcat cggaatcata aatcgaataa tttcttgtaa atccaatat
//
LOCUS       DAORVQ010000316         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_31977_length_2860_cov_5.483422, whole genome shotgun sequence.
ACCESSION   DAORVQ010000316 DAORVQ010000000
VERSION     DAORVQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31977_length_2860_cov_5.483422"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..760)
                     /locus_tag="PLX80_14515"
     CDS             complement(305..760)
                     /locus_tag="PLX80_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22050.1"
                     /translation="MSDNDVLNFGNGYIKSDITEDKLTDATDYALVDNNVLNFESAKN
                     PLLGDNYLRGEYRTSNLTEHFYENTGSEADENLIDLPKIFNLEINYPNPFNPSTAIKF
                     SLSPEVNVKLALYDITGREVSILVNENLKAGVFECQWNGSGLSSGVYFY"
     gene            1017..2750
                     /locus_tag="PLX80_14520"
     CDS             1017..2750
                     /locus_tag="PLX80_14520"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQY22051.1"
                     /translation="MRLTKFFLPTLKDDPVDAVTVSHKLMIRAGMVKQLTSGVYSTLP
                     LGLIVFRKVENIIRQEMNAIGGNEFLLPALSPHDLWSETGRMEDYGDLMFRIKNRELV
                     LCPTHEEVFTSIAKPSLISYKDFPQVWYQIQSKFRNEERPRAGVLRARQFTMKDAYSL
                     DATWEGLDKSYNDMAQAYRNIFTRCGLDFVIVSAHSGAMGGSASEEFMVPSSAGEDNI
                     VISEDGNYTSNIEVAVSFVEKNDRKNSDLPFEEFHTPNIKSIEELAGFLNITDKTRLA
                     KSRVFVNPSKEEKGEDEYVLVLVRGDDEVNELKLQNLFGINIRPAHNEELAEITGADA
                     GSIGPVNLKVLKNKKVKIIADLLLEDSDELVSGANKNDHHIKNIDIKRDVPGIEYKDL
                     RIVKEGELTSDKKSKLKITKAIEVGHIFKLGTKYPEALGAKFLDADGKENPIIMGSYG
                     IGVERTTASFIEQSNDDKGIIWKGEIAPFQINLICLSGKTDEVKDLAEKYYLELTEQK
                     KIEVLYDDREEVRPGVKFNDADLIGIPVQLIVGERNLKDGNVEIKIRQTGEKIIVSKD
                     DAVSKAIELIG"
BASE COUNT         1029 a          426 c          532 g          873 t
ORIGIN      
        1 caggaagctt ttttaaacta attgtaagtg aatcagaatt gatccctttt aattaaaaat
       61 aaaaatggaa taaaaaagat cattttgaaa atgaaacggg tcatacattg taaaaaatta
      121 cacctgctta agatgatact ttttagattc ttcattaaaa taaatttggg gattatttta
      181 aattcagtta aagttaaata aaaataaaac aagttcctgt catataattt atagacagga
      241 tctgaacaaa atattatttt gacaaaatca tttttttgac ctgagaaaaa ctttctgttt
      301 ttaattaata aaaataaaca cctgaagata aacctgatcc gttccattga cactcaaata
      361 cacctgcttt cagattttca ttcaccaata tagaaacttc tctaccggta atatcatata
      421 gagcgagttt tacatttact tctggagaaa gagaaaattt aattgcagta cttggattaa
      481 atgggttagg ataattaatt tcaagattga aaattttagg gagatcaatt aaattttcat
      541 cagcttctga tccggtattt tcataaaaat gctctgttaa atttgaagtt ctatattcac
      601 ctcttaaata attatctccg agcaaaggat ttttcgctga ttcaaaattt aatacattgt
      661 tatcaactaa agcatagtcg gtggcatcgg ttaacttatc ttctgttata tcagatttga
      721 tatagccgtt tccaaagttt aatacatcat tgtccgacaa tcaaccgtca gtcccatcaa
      781 caacaccgtc ctgatttaca tctcctccga tcagacaata caaagtccct tttagaatta
      841 aattatttcc atacgcctga aatttggaag gagtaaaatc ataatatgaa gccagacagt
      901 attgtctttt ataaaatatt aattctgtaa aaaattttag acttttattc tgatactaat
      961 attagaattg taatagaaac catacatttt tatattgtta gcttaaattt tagaaaatga
     1021 gattaactaa attttttctg ccgactttga aagatgatcc ggttgatgcc gtaacagttt
     1081 cacacaaatt aatgatcaga gccggaatgg taaagcagct tacttcaggt gtttattcta
     1141 ctttgccgct tggacttata gttttcagaa aagttgaaaa tattatccgt caggaaatga
     1201 acgcaatcgg tggtaatgaa tttttactgc ctgcattatc tcctcatgat ctatggtctg
     1261 aaacaggcag gatggaagat tatggtgatc ttatgttcag gatcaagaac cgtgaacttg
     1321 tattatgtcc tactcatgaa gaagtgttta cttctattgc aaagccaagt cttatttcat
     1381 ataaagattt tccgcaggta tggtatcaga ttcagtcaaa attcagaaat gaggaacgtc
     1441 ctagagcagg agttttaaga gcccgccagt ttacaatgaa agatgcgtat tcgcttgatg
     1501 ccacttggga aggtcttgat aagtcgtata atgacatggc tcaggcatac agaaatattt
     1561 tcacacgatg cggacttgat tttgtaattg tatcagctca cagcggcgca atgggtggaa
     1621 gtgcttctga agaatttatg gttccatctt cggcaggtga agataatatt gtaataagtg
     1681 aagatggaaa ttatacatcc aatatcgaag tcgcagtttc atttgtcgaa aaaaatgaca
     1741 ggaaaaattc tgatctgcct tttgaagaat ttcatacacc gaatattaaa agtatagaag
     1801 agcttgcagg attcctgaat attactgaca aaacaagatt ggcaaaatca agagtatttg
     1861 taaatccatc gaaagaggaa aaaggtgaag atgaatatgt gcttgtactt gtgagaggtg
     1921 atgatgaagt aaatgaattg aagctccaga atttattcgg aataaatatc cgacctgcgc
     1981 ataatgaaga actggcggaa ataacaggtg cagatgcagg ttcgataggt cctgtaaatc
     2041 taaaagtatt gaaaaataaa aaagttaaga tcattgcaga tcttttactt gaagacagtg
     2101 atgaactggt aagtggtgca aataaaaatg atcatcacat aaaaaatatt gatattaaaa
     2161 gagatgtccc gggtattgaa tacaaagacc tgagaattgt aaaagaaggc gagctaactt
     2221 ctgataaaaa aagtaaatta aaaattacaa aagcaattga agtcggacat atatttaagt
     2281 taggtacaaa atatcctgaa gcactcggcg caaaatttct tgatgcagac ggtaaagaaa
     2341 atccgatcat tatgggaagt tacggaattg gagttgaacg aactaccgct tcatttattg
     2401 aacagagtaa tgatgataaa ggaataatat ggaaagggga aatagcgccg tttcagataa
     2461 atctgatttg tctctctggt aagacagacg aagtgaaaga tcttgcagag aaatattatt
     2521 tggaattgac agaacagaaa aagatagaag ttttatatga tgacagggaa gaagtcagac
     2581 ccggtgttaa attcaatgat gctgatctga tcggaattcc tgtacagctt atagtcggtg
     2641 agagaaatct taaagacgga aatgtagaaa ttaagatcag acaaaccggt gaaaagatta
     2701 ttgtcagtaa agatgatgca gtttcaaaag caattgaact aatcggataa tatttggctg
     2761 acctgaaatc cattctgaat aaacaataca tccttgcctc tgcttcgccg agaagggcac
     2821 agcttctcag acaaatcggt ctaaaatttg tcactattga
//
LOCUS       DAORVQ010000317         2858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_32028_length_2858_cov_5.378523, whole genome shotgun sequence.
ACCESSION   DAORVQ010000317 DAORVQ010000000
VERSION     DAORVQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32028_length_2858_cov_5.378523"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2253)
                     /gene="pheT"
                     /locus_tag="PLX80_14525"
     CDS             complement(<1..2253)
                     /gene="pheT"
                     /locus_tag="PLX80_14525"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQY22052.1"
                     /translation="MKISLNWIKDYIPGLEFESLNSLTNKMIATGLDIESVEDEGSRF
                     NNFVVGEVLDKQKHPNADKLSLCKVDTGDGIVNIVCGAPNVDKGQKVCVAKVGAVIPN
                     GEFEIKKSKIRGEVSEGMICSGKELNLSDDHDGILVLQTEVKNGTPFADIMNLNDVVF
                     DIGITPNRGDLFSHFGVAREIAAIFNKKIELPNIEFYENGEDTNELINISIENTDLCK
                     RFTGRVIKNVKIGESPEWLKKRLISIGLRPRNNIVDITNFVMMETGQPLHAFDYDKIH
                     GKRIIVKTAEDGVKFTTLDSKERKLNSESLMICDGEGYSGIAGVMGGEYSEITDSTKN
                     IFLESAYFDPVSIRKNSKRLGLQTDASQRFERGVDIEIVKYASQRASKLISEIAGGEI
                     SKGLYDVFPEKFSQIETGVRISKANELIGIELKKDDIINLLEKIEINFSVESEGKLNF
                     KIPEFRREDISREADLIEEIARLYGYDNIPAKYNFNVNTENSSLIDEKSYRLMKFISE
                     YFEGRGFNQILTSPLSEGKEISISKSTDKPIRLENSVSAEMNTLRSGLLPGMLGVIRN
                     NFNNNGKSISLKLFEAGNVFAHSESKFTEEQKLILAISGKNDESVLFGGDKYFDIFDM
                     KGEAEMFLSKLNLDKLGLFYYYDNDISRNRIDISLNKNNIGFIYKADSKLKKEFEIDS
                     EVYIAEFCLNKILEYSFRTILYKEISKFPKVKRDLAVIVKSDTPFKEVSGQILHSGKG
                     LLEKLELFDIY"
     gene            complement(2450..>2858)
                     /locus_tag="PLX80_14530"
     CDS             complement(2450..>2858)
                     /locus_tag="PLX80_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22053.1"
                     /translation="SDFSELNAKSKTKVEDLNQSKTKKKTKKKSSKKKKSSSGKKKKS
                     SKKKISKKNKKSGKKYSKKKKKSGKKYSKKKKGSKKKYSSKKKNSSRKSYNTTEYNAP
                     KTYNAGNDGSSREYKRSEDKPVENFKKEPKKEN"
BASE COUNT          825 a          541 c          380 g         1112 t
ORIGIN      
        1 ataaatatca aataattcca gtttctcaag taaaccttta ccgctgtgaa gtatctgacc
       61 gctgacttct ttaaatggcg tatcactttt aactataact gccagatctc ttttgacttt
      121 tggaaatttt gagatctcct tatataaaat agttctgaat gaatactcta aaattttgtt
      181 aagacaaaat tcagcaatat aaacttcaga atcaatctca aattctttct taagtttgct
      241 atctgcttta taaataaaac cgatgttatt tttatttaaa cttatatcta tcctgttacg
      301 gcttatatca ttgtcataat agtaaaataa tccaagttta tcaagattta atttagacaa
      361 gaacatttcc gcttcacctt tcatatcaaa aatatcaaaa tacttatccc caccaaataa
      421 tacagattca tcattctttc ctgatattgc aagtatcagt ttttgctctt cagtaaattt
      481 actttccgaa tgtgcaaata catttcccgc ctcaaacaat ttaagagaaa tactctttcc
      541 gttattattg aaattatttc tgatcacacc aagcataccc ggcagtaatc ctgacctgag
      601 agtattcatt tccgctgata cagaattttc aagtctgatt ggtttatcag tactttttga
      661 aatagaaatt tccttacctt cagaaagggg agatgtcaat atctgattaa atcctcttcc
      721 ttcaaaatat tctgagatga atttcattaa tctgtaactt ttttcatcaa taagtgagga
      781 attttctgta ttcacattga aattatattt agcaggaata ttatcataac catataatct
      841 tgcaatttct tcaataagat cagcttctct tgaaatatct tcccttctga attcaggtat
      901 tttgaaattt aacttacctt cagactcaac tgaaaagttt atttcgattt tttctaaaag
      961 attaattata tcatcttttt ttaattcaat accaattaat tcatttgctt tggatattct
     1021 tacacctgtt tcgatttgtg aaaatttttc aggaaacaca tcatatagac ctttggaaat
     1081 ttctccgcct gcaatttcag aaatgagttt agaagctctc tgacttgcgt actttacaat
     1141 ttcaatatcc acaccgcgct caaatctttg tgaagcatcg gtctgaagtc cgagacgttt
     1201 ggaatttttt ctgatactga caggatcaaa atatgcagat tcgagaaaaa tgtttttagt
     1261 ggagtcagta atttcagaat attcaccacc cattacgccg gcaattcctg aataaccttc
     1321 accatcacag atcatcagcg attcgctgtt aagttttctt tcttttgaat caagtgttgt
     1381 gaatttgacg ccgtcttcag ctgttttgac aattatcctt ttaccgtgta tcttatcata
     1441 atcaaaagca tgaagcggct gacccgtttc catcattaca aaattagtta tatctacaat
     1501 attatttctc ggtctcagtc cgatcgagat aagccttttc ttcagccatt ctggtgattc
     1561 gccgatcttt acatttttaa tgaccctacc tgtgaatctt ttgcaaagat cagtattttc
     1621 aattgaaata tttatcaatt catttgtgtc ttcaccattt tcataaaatt caatattggg
     1681 aagttcaatt tttttattaa atatagcggc aatctcacgg gcaactccaa agtgtgaaaa
     1741 cagatcgcct ctgtttggag tgattcctat atcaaacacg acatcgttca gattcataat
     1801 atcagcaaat ggagttccat tttttacttc agtctgtaaa accaaaattc cgtcatgatc
     1861 atcagacaga ttcagctctt tacccgagca gatcattcct tcagaaactt ccccgcgtat
     1921 ctttgatttt ttgatctcaa attctccgtt agggattact gcacctacct ttgcaacaca
     1981 tactttctga cctttatcga catttggagc tccgcatacg atattgacaa ttccatcacc
     2041 tgtatccact ttacataaag aaagcttatc ggcatttgga tgtttctgct tatcaaggac
     2101 ttctcctact acaaagttat taaatctgct gccctcatct tcgactgatt caatgtccag
     2161 accggttgcg atcattttat tggttagtga attcaaggat tcgaattcca gaccggggat
     2221 gtaatctttt atccagttta aactaatttt catagttagc aatatactta ttcaattttc
     2281 aatgttcaat ttacaatgta caattcaaat cattaaaatt tcatagatca atgttttttt
     2341 taaataaaat aaaacaacca tttcaaaatt caatcagttt gcacaaaaaa aaagcccggt
     2401 tattatgacc gggctattat taaatattga attttttatt ggtttagaac taattttcct
     2461 ttttcggttc ttttttgaaa ttttccactg gtttatcttc gctcctttta tattctctgg
     2521 atgagccgtc atttccggca ttgtaggttt ttggagcgtt gtattcagtt gtattgtaag
     2581 attttctgga actgtttttc tttttggaac tgtatttctt cttactgccc tttttctttt
     2641 tagaatattt ctttccggat ttctttttct ttttagaata tttctttccg gatttcttgt
     2701 tctttttgga aattttcttt tttgaagatt ttttcttttt acctgaagaa cttttctttt
     2761 tctttgaaga tttctttttt gttttctttt ttgttttact ttgattcaga tcctcaactt
     2821 tagtcttgga tttagcattt aactctgaaa aatcacta
//
LOCUS       DAORVQ010000318         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_32761_length_2822_cov_4.507047, whole genome shotgun sequence.
ACCESSION   DAORVQ010000318 DAORVQ010000000
VERSION     DAORVQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32761_length_2822_cov_4.507047"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..357
                     /locus_tag="PLX80_14535"
     CDS             229..357
                     /locus_tag="PLX80_14535"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HQY22054.1"
                     /translation="MKVGRQITINQRYELIIHGKDGKITDKDNYSNDMCIHKVKKH"
     gene            complement(445..945)
                     /locus_tag="PLX80_14540"
     CDS             complement(445..945)
                     /locus_tag="PLX80_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQY22055.1"
                     /translation="MAKQKTLKDFLIEELKDLYSAEKQITKVLPKMAKAATSDELKKA
                     FEDHLKETENQIERLDKISKLIGKSLTGKKCKAMEGLANEGKEIIEEDMEDEVRDVAL
                     ICGAQKVEHYEMASYGCARTYAGLLGMDDIKDLLQETLDEEGAADKKLTEIAETLNVE
                     AMSETE"
     gene            complement(1052..1252)
                     /locus_tag="PLX80_14545"
     CDS             complement(1052..1252)
                     /locus_tag="PLX80_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22056.1"
                     /translation="MDKKYKSSFIKTKEELEYDFRILNSNSIIEENSISNLNYLENKI
                     NDSIKKNSSLIKVTLKNSIEFK"
     gene            1550..>2822
                     /locus_tag="PLX80_14550"
     CDS             1550..>2822
                     /locus_tag="PLX80_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22057.1"
                     /translation="MKKIFKKLFKIFLWLTGLFIIFLVALFIFIQTDMFNNLALDFAT
                     KKLNEGWKEKESVISAGSISGNIFSNLKLEDILITVKSDTIIKINTLSLKYDIWGLLD
                     KQIRLENVTIDSPDINFLKIKDSSGSLIWNFSKLFSSAEEDKDTTGSAFDWDIALNDL
                     KILNGSFTTNTPVQKQTDSLTLQNDSLNNFNFENLKVSDLNADLNAEYFTDNKILNLN
                     NLSFKTRSGFRIDTLSFKTEINLKDTTIDLDNLKFITGRSDIRINKIHINKLNPFDST
                     TFINFKEKFFEADFNIAKLDFDDVKYFIPDVEYLDSTISLTINAKGKYGDFDLADLTL
                     RLPDSYINLTGNVQNLDEPDSLYLDIIANDLSIQTADIKKIYKDNSMEDYSHLGKVNA
                     DITFTGTVNKFFTQFDINTNAGSSDGILNLDL"
BASE COUNT         1009 a          392 c          384 g         1037 t
ORIGIN      
        1 aaaaattaag ttttaactgt taattagaat cttaataatt aatttgaaat tgaattttag
       61 aaactatgat gaaaaattca attttatgat atttaaagga agatatgatt tttgaaaaaa
      121 aataatgaaa aaaataacca gcatattatt cctttaggta acggatgggc tgtgaaataa
      181 gaaaaaagca atagatttaa gataattaca gaattaaaga atactgcaat aaaagtcgga
      241 agacaaatta ccataaatca aagatatgaa cttattattc atggaaagga cggaaagatt
      301 actgataagg acaattatag taatgatatg tgtatccata aagttaagaa acattaagga
      361 tttaaaaata ggaaagaaat ctaaatcagc acctgaaaat aaattcaggt gctgaataga
      421 aaataaatta aagatttttt gattttattc agtctctgac atagcttcaa catttagagt
      481 ctcagctatt tctgttagtt ttttatcagc agcaccttcc tcgtctaaag tttcctgcag
      541 aagatctttt atatcatcca ttcccaaaag tccggcataa gttcttgcac atccatatga
      601 tgccatttca taatgttcga ctttctgtgc tccgcagatc aatgcaacat ctcttacttc
      661 atcttccata tcttcttcaa ttatctcctt accttcattt gccagtcctt ccatagcttt
      721 acattttttt ccggtaagag attttccgat cagttttgat attttatcca gtcgttcgat
      781 ctgattttca gtttctttca ggtgatcctc gaatgccttt tttaattcat cagatgtcgc
      841 tgcttttgcc atctttggca gaacttttgt tatctgcttt tctgcgctgt aaagatcttt
      901 taattcttca attaaaaaat ctttgagtgt tttttgcttt gccattttga ttttgttttt
      961 taattattaa aaaaagttgt ttattatttt atttaaagcg aattactata aatttttaag
     1021 taaaagatcc ggcagatcta ttaattaatg ttcatttaaa ttctattgaa tttttaagtg
     1081 taaccttaat cagactacta ttctttttaa tggaatcatt tattttattc tccagataat
     1141 ttaaatttga tattgagttt tcttctatga ttgaattaga atttaatatt cgaaaatcat
     1201 attcaagttc ttcttttgtc tttataaaag aagatttata ttttttgtcc atttcaatta
     1261 ttaatttaat ttttttattt tactatattt actgacaata tccgtgccat tgaataaaag
     1321 taaaatttca tttaaaaaaa gaaataattt tttcaattaa taaatttttt tgacaaaatt
     1381 gacatatttc tgacattaat tctaacagta agccaacagt taactacttc taattttatt
     1441 tctttatagc attacaatga aatggactaa aaagaattga ttttatgcat ttgtaattga
     1501 tttgaaaaat ttaaaattac ggcatacaaa atgatacttt aatatctgaa tgaaaaaaat
     1561 atttaaaaag ttatttaaaa tatttctgtg gctgaccgga ctctttataa tatttctggt
     1621 tgcactgttt atttttattc agacagatat gtttaataat cttgcactgg attttgcaac
     1681 taaaaaatta aatgaaggat ggaaagaaaa ggaaagtgta atatccgcag gatcaatcag
     1741 cggtaacata ttttccaatc tgaagcttga agatatttta attactgtaa aatctgatac
     1801 gatcataaaa attaatacac ttagtttaaa atatgatatc tggggcttgc tcgataaaca
     1861 gatcaggtta gaaaatgtca caattgattc acctgatatt aattttttaa aaataaaaga
     1921 ctcttcagga tctctgatat ggaatttttc taaattattc agctctgccg aagaggataa
     1981 agatacgacc ggatctgctt ttgactggga tattgcacta aatgatttga aaatattaaa
     2041 cggatcattt actactaata ctccggttca gaaacagaca gactcattaa cacttcaaaa
     2101 cgacagttta aataatttta attttgagaa tttaaaagtt tccgatctga atgcagatct
     2161 aaatgcagag tattttacag acaataaaat tctaaatcta aataaccttt cttttaaaac
     2221 tcgatcaggc ttcagaattg atacactaag ttttaagaca gaaataaatc taaaagatac
     2281 aactatagat ttagataacc ttaaatttat taccggcaga tctgatatta gaataaataa
     2341 aatccatata aataaattaa atccttttga cagtactact tttattaact ttaaagagaa
     2401 attcttcgaa gctgatttca atattgcaaa actggatttt gatgatgtga aatattttat
     2461 ccctgatgtt gaatatctgg acagtaccat ctcactaact attaatgcca aaggaaaata
     2521 cggagatttt gatttagcag atctgactct cagacttccc gattcatata tcaatcttac
     2581 cggaaatgtt cagaatcttg atgaacctga tagtttatat cttgatatta ttgcaaatga
     2641 tcttagtata cagacagccg atattaaaaa aatatataaa gataattcta tggaagatta
     2701 ttcgcatctt ggaaaagtaa atgctgatat tacttttaca ggtacggtga ataaattctt
     2761 tacacagttt gatattaata caaatgcagg ttcttcagac gggattctga atcttgatct
     2821 ga
//
LOCUS       DAORVQ010000319         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_33270_length_2799_cov_4.287901, whole genome shotgun sequence.
ACCESSION   DAORVQ010000319 DAORVQ010000000
VERSION     DAORVQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33270_length_2799_cov_4.287901"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1724
                     /locus_tag="PLX80_14555"
     CDS             291..1724
                     /locus_tag="PLX80_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY22058.1"
                     /translation="MNLEKGNITISDLKNIIGLKDLPDDHLQWILINSEYTEFEDGFQ
                     INKKGDPIDFLWIILEGKINYNIEINGTQVHFYTFENDNATGGIGGLIPYSRMKSSPG
                     FAYAVGNVKRLELHKDHFSELEKLNPDFVQRLIGYMTERARAFATVKLQHEKVNALGK
                     LAAGIAHELNNPASAINRISSELDKRLTNNYRLTEELLNSNVKSEQIKMVRELVEKRE
                     SETGNKQKLSLSEKMDLEDEIYEWLNRKGLSEDKIIGETFAESGFSVSDLDDFCKEFN
                     KENFSQMIQWLENLLSSKKILTDLEEASSRISHLVGAIKSHVHMDQANELQPTDIHKD
                     IDNTLTLLGYKLSEKNITVKKIYCEKPVEMNVYIGELNQVWTNLIDNSIYALEKNGEL
                     VIETICSDKNFIVKLTDNGSGIPQDIISRIFDPFFTTKKVGEGTGIGLDLVNRIIKNH
                     NGEISVNSKPGNTEFKISLPVSEIKKL"
     gene            1728..>2799
                     /locus_tag="PLX80_14560"
     CDS             1728..>2799
                     /locus_tag="PLX80_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015135722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY22059.1"
                     /translation="MKLPYILIVDDDEQVLRAILRDVRSKYRDEYRVNASESATEALE
                     VIAKLKQKNETVALFISDQRMPEMQGIEFLEKAKKIFPEAKMVLLTAYSDIDAAIKAI
                     NDIKLDFYLLKPWNPPEEKLYPVLDDLLYEWSAHINLPYDGIRVLGSRWSGVCYSAKE
                     FLSRNLIPYQWIDIDIDEKMKEQALSLTGDINNLPVILFPDGTSLINPSNNEIAEKVG
                     LQTKAKLDFYDLIIIGAGPAGLAAAVYGTSEGLKTLIIEQNAPGGQAGTSSQIENYLG
                     FPTGISGEDLARRAVTQAKRFGAEILTQEVVSIKINQPYKILKLKNGIEVSSYAVVFT
                     AGVSVRMFEKDGIEKFIGAGVYY"
BASE COUNT         1000 a          427 c          545 g          827 t
ORIGIN      
        1 tctgttttgc aaaacccttt tgttgtaagt atgtgaaact attaaaatcc gaattatgaa
       61 tcacggacgg aaacaaaaaa gcatttttag aactggcttc gattttctaa ctacattcat
      121 taaaaagctc ttaacgggaa atttcagatt gaaagagtat atttcttcaa actgaaaatt
      181 agcttatttt actttacttg cagtttacct gaactgttaa ttttttaata ctataattta
      241 ttcagttcat aaaaaaaatg taaaatcttt tttaacgttt tttaaaatat ttgaatttgg
      301 aaaaaggaaa tattacaata tcggatttaa aaaatattat cgggcttaaa gatctgcctg
      361 acgatcatct gcaatggata cttattaatt cggaatatac agaatttgaa gacgggtttc
      421 agatcaacaa aaaaggtgac ccgatcgatt ttttgtggat aatacttgaa ggtaaaataa
      481 attataatat tgaaatcaat ggaacacagg ttcattttta tacttttgaa aatgataatg
      541 caacgggagg gataggagga cttatcccat attcaagaat gaaatcttca cccgggtttg
      601 catatgctgt tggaaatgtt aaaaggttag agcttcacaa agatcatttt tctgaactgg
      661 aaaaattaaa tcccgatttt gtgcagagac tgatcggtta tatgacagaa agagcccgag
      721 cttttgcaac agtgaaatta cagcatgaga aagttaacgc tcttggaaaa cttgctgcag
      781 gtatagctca tgagcttaat aatcctgctt cagcaattaa cagaatttct tcagaacttg
      841 ataaacgtct tacaaataat tacagactta ccgaagagct tctgaattct aatgtaaaat
      901 ctgaacagat taagatggta agagaacttg ttgagaaaag agaaagtgag acaggaaata
      961 aacaaaagct gagtcttagc gaaaaaatgg atctcgaaga tgaaatatat gagtggctga
     1021 atagaaaagg actttcagaa gataagataa tcggagaaac atttgcagag tcaggatttt
     1081 cagtaagtga tctggatgat ttttgtaaag aatttaataa agaaaatttc agccaaatga
     1141 ttcaatggtt ggaaaattta ctcagttcaa aaaaaatatt aactgatctt gaagaagctt
     1201 cttcacgcat ttctcatctt gttggtgcga tcaaaagcca tgtgcatatg gatcaggcaa
     1261 atgaactcca gcctactgac attcataaag atattgacaa tactctgact ttgcttggat
     1321 acaaacttag cgaaaagaat attacagtaa aaaaaatata ctgcgaaaaa cctgtcgaaa
     1381 tgaatgtata tattggagag cttaatcagg tctggacaaa tttaatagac aattcaattt
     1441 atgcacttga aaaaaacggt gagctggtca ttgagactat atgcagtgat aaaaatttta
     1501 tcgtaaaatt gactgacaac ggttctggaa ttccacagga tattatctca agaatatttg
     1561 atcctttttt tactactaaa aaagtcgggg aaggtacagg cattggatta gatcttgtaa
     1621 acaggatcat taaaaatcat aacggtgaaa tttcagtaaa ctcaaagcct ggaaatacag
     1681 aattcaaaat cagtttacct gtatcagaaa tcaaaaaatt ataatctatg aaactcccat
     1741 atatattaat agtcgatgat gatgagcagg tgcttcgcgc aattcttcgt gatgtaagaa
     1801 gtaagtaccg cgatgaatac agagtgaatg catcagagtc tgctacagaa gcattggaag
     1861 tcattgctaa attaaaacaa aaaaatgaaa ctgtagcttt atttatttcc gatcagagaa
     1921 tgccggaaat gcagggtata gaatttcttg aaaaagccaa gaaaatattt ccggaagcta
     1981 aaatggtttt gcttactgca tattcggata ttgacgctgc cataaaagca ataaatgata
     2041 tcaagctgga tttctatttg ctgaaaccct ggaatccacc tgaggaaaaa ttatatcccg
     2101 ttttggatga ccttctttat gaatggtctg cccatatcaa tctgccttat gatgggatca
     2161 gagttctggg ctcaagatgg tcgggtgttt gctactctgc aaaagaattc ctctcaagaa
     2221 atttaattcc atatcagtgg atagatatag acattgatga gaaaatgaaa gaacaagcgc
     2281 tttcattaac aggagacatt aacaatctgc cggttatttt atttccggat gggacatcat
     2341 taattaatcc ttcaaataat gaaattgccg aaaaggttgg tttacagaca aaagcaaaac
     2401 tggattttta tgatctgata attataggtg cgggacctgc cggtctggct gctgctgtgt
     2461 acggaacttc agaaggactc aaaactctta tcatcgaaca aaatgccccg ggcgggcagg
     2521 cgggtacaag ttcgcagatc gaaaattatc tcggatttcc gacaggtatc agcggagaag
     2581 atcttgccag aagagcagta actcaggcaa agaggtttgg tgctgagata cttacacagg
     2641 aagtggtttc aataaagata aatcaaccat ataaaatatt aaagctcaaa aacggcattg
     2701 aagtatcttc atatgctgtg gtctttactg ccggtgtttc tgtacgaatg ttcgaaaaag
     2761 atggcattga aaaatttatt ggtgcaggtg tttactacg
//
LOCUS       DAORVQ010000320         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_33748_length_2777_cov_6.282880, whole genome shotgun sequence.
ACCESSION   DAORVQ010000320 DAORVQ010000000
VERSION     DAORVQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33748_length_2777_cov_6.282880"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLX80_14565"
     CDS             <1..315
                     /locus_tag="PLX80_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22060.1"
                     /translation="GYLSSDVNGDAFIDLADYAFTDNNAANFVGVIVPPAPPPAGELN
                     ETKDSAPSAVGDPSIGNSNLIINRNYETDTDSKEDLINFNENYKTGRKISQEEIDKAD
                     GK"
     gene            487..>2777
                     /locus_tag="PLX80_14570"
     CDS             487..>2777
                     /locus_tag="PLX80_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY22061.1"
                     /translation="MKMKINSLIFSMLLFSSFFISPDISNANLYWNQAASFAGSSSSY
                     LAVRDDADLDITGDFTLETWVNPTNAVSPSIQIIIGKGQGSPSGYFIYLFNGKVAIGT
                     QNIVRIVGNIVIPSNKWTHIAGTYTASLNRFIIHVNGVVDSVKTVGAAAPLANNDSLL
                     IGKGAGGRFAGMLDEVRIWNTDNSAFIYKYHRTSLAVSSGKYAGLVYSLTFQDDDNSG
                     NAFSLQDFAFKNSSANNIGVSIVDLSNRPSSTIHTNDCINLDGNNDYLASPDSPEISP
                     TSGITVSAWILPSSNVNSVIIQKGSPAGGLGTDYRLALLNGNLFAGINGSLFIPGSVS
                     EVPLNQWSYVAFTYEGSSGDYKFYINGKNVYNGNQNLGNINNGSDSLYIGGTLNLNDF
                     DGNIDEISIVPEAKSEEFIGDNMFRSRNLANVNSQNEVVYNFDGYSVSNSGVITDIGK
                     QMYFRNNAEFTHNGGVNNAPESPMIGMNIMAFQEGFRLKSSDKSIPETGSIGSMISDT
                     LEILQSGSVTDINFFIALNHTREIDLQITLTNPSGNTVNVFDNITYLGSSGNLVTIFD
                     DQADSSLLTDRYAHLAPKVKPLNTMISVFGLQSMKGKWILNITDQFAGASGRLYGWGM
                     QFNNQTSKPKKLNMTAFIQGFYNPVMSQMIRDTIKFYLRDQFSPYATVDSSKDYATSV
                     GFSDVNFNNAVAGRPYYLTIKHRNLIETWSSSTVYFDQLTYELKYDFSKNPSSAFGNN
                     LIQVDDTPVRFASYNGDVNQDQT"
BASE COUNT          938 a          428 c          546 g          865 t
ORIGIN      
        1 ggttatctta gttcggacgt aaacggagat gcatttattg atctagcaga ctatgccttt
       61 actgataata atgctgctaa ttttgtaggt gttatagtgc cgcctgctcc gcctcctgca
      121 ggagagctga atgaaactaa agattctgca ccttctgctg tcggagatcc gagtattgga
      181 aatagtaatt tgattataaa tagaaattat gaaaccgata cagattcaaa agaagatcta
      241 attaatttta atgaaaatta taagaccgga agaaagatct ctcaggaaga aatagacaaa
      301 gctgacggga aataaaaatt aattttttta tttaaagtta aacgaatttt tattatttaa
      361 tagaacggta acttaggtta ccgttttctt tttaaaaatc tgaatacaga atattctatc
      421 caaataaatt taatgatttt tcataatcag tgaatcagac tcaattaaat aaacgaaagg
      481 aaaaaaatga aaatgaaaat taattcttta attttttcaa tgctgctgtt tagtagtttt
      541 tttatatctc cggatatttc aaatgcaaat ctgtactgga atcaggcagc ttcatttgcg
      601 ggttcaagca gcagctatct tgcagtcaga gatgatgcag atctggatat tacaggtgac
      661 tttacattag aaacctgggt gaatccaaca aatgcagtgt ctccgtcaat tcagataatt
      721 ataggaaagg gtcagggaag tccatccggt tatttcattt accttttcaa tggaaaagta
      781 gctattggaa cccaaaatat tgtacgtatt gtgggaaata ttgtgatccc aagtaataaa
      841 tggacgcata ttgccggtac atatacagct tccttaaata gatttattat acatgtaaat
      901 ggagttgtag attccgttaa aacggttgga gctgctgctc ctcttgccaa caacgattct
      961 cttttgatcg gaaaaggtgc aggcggtcga tttgcaggaa tgctggatga agtaaggatc
     1021 tggaatacag ataactcggc atttatctat aaatatcaca gaacaagtct tgcagtaagc
     1081 agtggaaaat atgccggact tgtatattct ctgacgtttc aggatgatga taacagcgga
     1141 aatgcatttt cattacagga ttttgctttt aagaactctt cagccaataa catcggagta
     1201 agtatagtag atcttagcaa cagaccttcc agtacaattc atacaaatga ttgtataaac
     1261 ttagacggca acaatgatta tctggcatcg cccgattctc ctgaaatcag tccgacttcc
     1321 ggtattacag ttagtgcatg gatcctgcct agttcgaatg tcaattcagt cattatacaa
     1381 aaaggatcgc cggcaggcgg attggggact gattatcgac tggctttatt aaacggtaat
     1441 ttatttgccg gaattaacgg atcattattt attcccggaa gtgtaagtga agttccttta
     1501 aaccaatggt cttatgttgc atttacatat gaaggcagca gtggtgatta taaattttac
     1561 ataaacggaa aaaatgttta taacggtaat caaaatcttg gaaatataaa taatggttca
     1621 gactcgttgt acattggagg tacactgaat ttaaatgatt ttgacggaaa tattgatgaa
     1681 atttcaatag ttcctgaagc aaaaagtgag gaatttattg gtgataatat gttcagatca
     1741 agaaatcttg caaatgtaaa ttcacaaaat gaagtggttt ataattttga cggatattct
     1801 gtttctaatt caggagttat aactgatata ggaaaacaaa tgtattttag aaataatgca
     1861 gaatttacac ataatggcgg agttaataat gccccggaat caccgatgat aggaatgaat
     1921 atcatggctt ttcaggaagg atttcgctta aagtcatctg acaaaagcat cccggaaaca
     1981 ggatccatcg gaagtatgat cagtgatact ctggagatat tgcagagcgg ttcggttact
     2041 gatattaatt tttttattgc attaaatcat acacgtgaaa tagatctgca gatcacttta
     2101 actaatccgt cagggaatac tgtcaatgtg tttgacaata ttacatatct tgggtcatcg
     2161 ggaaacttag ttacaatatt tgatgatcag gcagacagtt cattacttac agacagatat
     2221 gcacatcttg ctccaaaggt caagccgtta aatacaatga tttcagtatt tggtttacaa
     2281 agcatgaaag gaaaatggat attaaatatt acggatcagt ttgcaggagc cagcggcaga
     2341 ttgtacggct ggggaatgca gtttaataat cagacttcga aacccaaaaa gcttaatatg
     2401 actgcattta ttcagggatt ttataatcct gtaatgagcc agatgataag agatactata
     2461 aagttttatt taagagatca attttcacct tatgctacag tcgattcatc caaagattat
     2521 gcaacatcag ttggattttc agatgtaaat tttaataatg cagttgccgg cagaccgtat
     2581 tatcttacaa taaagcatag aaatctaata gagacatgga gctcttcgac tgtgtatttc
     2641 gatcagctta cttatgagtt gaaatatgat ttttcaaaaa atccatcaag tgcatttggg
     2701 aataatctga tacaggttga tgatacacct gtaagattcg caagctataa tggagatgta
     2761 aatcaggatc agacaat
//
LOCUS       DAORVQ010000321         2771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_33890_length_2771_cov_2.584683, whole genome shotgun sequence.
ACCESSION   DAORVQ010000321 DAORVQ010000000
VERSION     DAORVQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33890_length_2771_cov_2.584683"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..695)
                     /locus_tag="PLX80_14575"
     CDS             complement(156..695)
                     /locus_tag="PLX80_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQY22062.1"
                     /translation="MEKNLNKNFIENSVSEFRKIKDLGDKAFSQIKDEDFFWSPDEES
                     NSIAIIVRHLSGNMLSRWTEFLTSDGEKPNRNRDEEFEKIFYTDKDDIISRWETGWSC
                     LFKALGSLKEEDLFKEVTIRNEPHTVLQAISRQLVHYSYHTGQIVYIAKHLENSKWKS
                     LSIPRGGSEEFNEKMKTKK"
     gene            complement(785..2161)
                     /locus_tag="PLX80_14580"
     CDS             complement(785..2161)
                     /locus_tag="PLX80_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQY22063.1"
                     /translation="MFAQEKVELKNANELSGKVIDGQNVREANGNVEFVQGNVKVFCN
                     SATQFIEANRIELRGNVRIYQDTLTLLTSKAVYFGDDKRAICEGGVTLKDPNATIRAD
                     NGIYFFSEAKAIFKGDVIIVNEEYRITSQELTYLRNTEDSFAKGNVVVTSDSAVIKAD
                     NIDFYKTQGKSYALGNVSIESDSTIITADTSTNYSREKRSVASGNVKITSLNNSTVIY
                     GNNIENFEINNYTILKGNARLVQIETDSLNSGKDTLLIYSDTMEAYRGKPDKYIAKSN
                     VEILRNEFSAKCNEGIYFKESETVSLSGKPIVWQDLLQMTGDSIFAELPENRLQTIFV
                     KKIPVENSMTSFVISGNNDEFFSDRYDQISGDDIEIKFKDDKIDLIEVKNNSNSIYFI
                     YENNKANGLNKVEGKDLYVYFDEEEKVSKIKVDTDPVGEYVPEQILNSTGLTLPGFDL
                     RNDKPVRK"
     gene            complement(2352..>2771)
                     /gene="lptC"
                     /locus_tag="PLX80_14585"
     CDS             complement(2352..>2771)
                     /gene="lptC"
                     /locus_tag="PLX80_14585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQY22064.1"
                     /translation="LPSQESWNSTVTFSDSGKVKAVLKAGHILVYSKKGFTLIDSGAV
                     VDFYKDGEIASTLSGNRGKINDKTKDIEMYDSVQVVSKDGSELKTQKLLWTNLTQRAS
                     SDVFVSIKTPTETIEGIGFESDQNLKNYKIYKVSGTF"
BASE COUNT          789 a          544 c          441 g          997 t
ORIGIN      
        1 acttatttca atggaagcag agaaaagcga tgtcataaaa gattttaatt ataaaatgga
       61 ttttacttca tttgaaatag ttggtatccc gttttacaat acaggaaacg gacaaatgaa
      121 agatgcattg cttgggttaa ctatctgatc aataattatt tttttgtttt catcttttca
      181 ttgaactctt cagatccgcc tctcggaata gaaagactct tccacttaga attctctaaa
      241 tgcttggcaa tatatacaat ctgaccggta tgatagctgt agtgaactaa ttgtctgctg
      301 atagcctgaa ggacagtatg aggttcgttt cgtattgtca cttccttaaa aagatcttct
      361 tctttcaaag atcccagagc tttaaacaaa caactccagc ctgtttccca tctcgaaatt
      421 atatcatcct tgtcagtgta aaaaatcttt tcaaattctt catcccggtt tcggtttggc
      481 ttctctccgt cagacgtaag aaattctgtc cagcgggaca gcatattacc gctgagatgc
      541 cttactataa tggctatgct gtttgattct tcatccggcg accagaagaa atcctcatcc
      601 ttgatctgtg aaaaagcttt atctccaagg tctttgatct tccggaattc tgaaactgaa
      661 ttttcgataa aatttttatt tagattcttt tccatagttt ccctgttttt ttttactgtt
      721 aaattttttt aattaaatat aataaatatc aatgtaactg ctttgattac ttcaatgcgt
      781 tacatcactt tctcacgggt ttgtcattcc tcagatcaaa acccggtaat gttaatcctg
      841 tcgaatttaa tatctgctcg ggaacgtatt ctcccacagg gtcagtatca accttgatct
      901 ttgatacttt ttcctcctca tcaaagtaaa catacagatc ttttccttca actttattca
      961 gaccgtttgc tttattgttt tcataaataa aataaatgct gttggaatta ttcttaactt
     1021 ctatgagatc aatcttgtca tctttgaatt ttatttcaat gtcgtcgccg cttatctgat
     1081 catatctgtc tgaaaaaaat tcatcattgt taccggaaat aacaaaagaa gtcatagagt
     1141 tttcaaccgg gatctttttt acaaaaatgg tttgcagcct gttttccgga agttcggcaa
     1201 agattgaatc acctgtcatc tgaagcagat cctgccatac aatgggtttt ccggaaagag
     1261 aaacggtttc actttcttta aaataaatac cctcattgca ctttgctgaa aattcattcc
     1321 ttaatatttc aacattactt ttcgctatgt atttatccgg tttgcctctg tatgcttcca
     1381 ttgtatcgct gtaaatcaga agagtatctt tgccggaatt gagcgaatca gtttcgatct
     1441 gaacgagtct tgcattgcct ttaagaatag tgtaattatt aatttcaaag ttctcaatgt
     1501 tatttccata aatgacagta ctgttattga gacttgtgat ctttacattt ccggaagcaa
     1561 ctgatctttt ctcccgggaa tagttagtag aagtatcagc ggtaataatt gtagaatcac
     1621 tttcaatgct tacatttcca agagcatagg attttccctg agtcttgtaa aaatcaatat
     1681 tgtcagcttt aatgaccgca gagtctgaag ttaccactac attacctttt gcaaaagaat
     1741 cttcagtatt tcttaaatag gtgagttcct gtgaagtgat cctgtattct tcatttacaa
     1801 ttattacgtc gcctttaaat atcgcttttg cttctgagaa aaaatatatt ccgttatctg
     1861 ctcttatggt tgcattcggg tccttcaatg ttacgcctcc ttcgcagata gctcttttgt
     1921 catctccgaa ataaacggct ttagaagtta gcagcgtaag tgtgtcctga tatatcctga
     1981 cgttaccgcg aagttcgatc cggttggctt ctataaactg tgtagcagag ttgcaaaata
     2041 ctttcacgtt accctgtacg aattctacat tgccgtttgc ttctctgacg ttctgcccgt
     2101 ctatcacttt tcctgaaagt tcattggcat tttttaattc aactttctcc tgagcaaata
     2161 ttacactaca tgtaaaacta catataaaaa aagtaaataa tatttttaaa attaatttca
     2221 ggatggttaa ttggttaaat ggttaaatgg ttaaatgata aatcggtaaa tcgaataatt
     2281 tgatttaaat cttaattata caatcagtcg catcggattt caaactttac aaactttaca
     2341 aactttacaa actaaaaagt cccgcttact ttgtagatct tataattctt cagattctga
     2401 tcagattcaa atccgattcc ttctatagtt tccgttggcg tttttatact tacaaataca
     2461 tccgaagaag ctctctgagt caggtttgtc caaagcagtt tctgagtctt gagttcgctg
     2521 ccatctttac ttactacctg cacgctgtca tacatttcaa tgtcttttgt cttgtcatta
     2581 attttccctc tattaccgga taaagtggat gcgatctcgc catccttata aaaatcgaca
     2641 actgccccgc tgtcaatgag tgtaaatcct ttttttgaat aaacaagtat atgaccggct
     2701 ttcagaacag cttttacttt tcctgaatca gagaatgtaa ccgttgaatt ccagctctcc
     2761 tgtgaaggga g
//
LOCUS       DAORVQ010000322         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_35384_length_2706_cov_4.322897, whole genome shotgun sequence.
ACCESSION   DAORVQ010000322 DAORVQ010000000
VERSION     DAORVQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35384_length_2706_cov_4.322897"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLX80_14590"
     CDS             <1..413
                     /locus_tag="PLX80_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22065.1"
                     /translation="GYLSTDTLMGATLVMQAAGGKIWGTFPFNESLFLGGEGSLLGFA
                     RERFAGNGLIMAQSELRIRLLPLNILVPGILGISVFGGTGRVYLQGEKSKTWHNSYGG
                     SVWISYINKLANFGITVAQSDEGFKFYLGSAFTL"
     gene            492..1544
                     /locus_tag="PLX80_14595"
     CDS             492..1544
                     /locus_tag="PLX80_14595"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQY22066.1"
                     /translation="MNFILKNKEKLRSVFIVTLSGVIVAPVFTAITFGLNPVTLLKGL
                     LIGFMITFVSSLIENFVFKNNFNKLKFSVTLFIRTIFYIFIISFSVLTVWVVHESSLN
                     DESLLNTIKSEDFRKFILTGDFKNIFFFAIAASFVINFFMQINSLLGKKVLLNYITGK
                     YHKPRIEFRTFMFLDLTSSTTIAEKLGPVKYHNFINNFFFDIDEAIVKFKGEIYQYVG
                     DEVIVSWIDNTGFEKNSCVNCFLEMKNIISRLSKNYINRFGYIPEFKAGVHYGEVVTG
                     EIGDSKKEIVFHGDVLNTASRIQAFSKTLDKDILISEEVQRKLNKESNMRIENIGKYK
                     LKGKEIELELFSVEFF"
     gene            complement(1521..>2706)
                     /gene="ppk1"
                     /locus_tag="PLX80_14600"
     CDS             complement(1521..>2706)
                     /gene="ppk1"
                     /locus_tag="PLX80_14600"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQY22067.1"
                     /translation="KYHNFQDFLGFPFPDIKHCVYQHKEELKTKYIYKSKNIFQTISE
                     KDQILHFPYESFSPVTDFISAAADDETVENIKITLYRVAKNSSIVNSLLNALKNGKRV
                     TVFIEIKARFDEELNIVYAGELQKAGAHVLYSIPGIKVHAKLAMVTRIENEVPVNYCY
                     LATGNFNEKTSRIYCDTGLFTSSKNITEEVSKIFDFLEGKIIDTEFKNLLVGQFNMKD
                     EFLKLIDSEIKNADKGKEASVILKLNSLEDRKMIDKLYEASKAGVKIFILVRGICCLI
                     PGVKKQSSNIKIISIVDKYLEHSRVFIFHNGGDKKVYISSADWMKRNLNRRIETAIPV
                     YDEDLKMELIDILDIQIRDNLKARIIDKDQKNEYINSGSNNNYRAQSDIYTYLSEKFY
                     TE"
BASE COUNT          903 a          373 c          435 g          995 t
ORIGIN      
        1 gaggatattt atctactgat acacttatgg gtgctacttt agtcatgcag gctgcaggcg
       61 gaaaaatatg ggggacattt ccatttaatg aatcattatt tcttggtgga gaaggttcac
      121 ttcttggatt tgcaagagag agatttgcag gtaacggttt aataatggca cagtcagaat
      181 tgagaatcag attattgccg ctgaatattc ttgtgccggg aattttagga atttcagtat
      241 ttggcggtac cggaagggtt tacctacagg gagaaaaatc aaagacttgg cataattcat
      301 atggaggttc tgtctggata tcatacataa ataagttagc aaattttgga attacagtag
      361 ctcaatcaga tgaaggtttc aaattttatc tgggatcagc atttacatta tagttttatt
      421 tgaaataaaa atcaaaaaaa tcatttaagt tataataaat aataacgtct gagatttttg
      481 taaatatagt tatgaatttt attctaaaaa ataaagaaaa gttaagatca gtattcattg
      541 taactctttc aggtgttatt gtagctccgg tatttactgc cattacattt ggtttgaatc
      601 cggttactct gctcaaagga ttattgatcg gttttatgat cacatttgta agttctctaa
      661 ttgagaattt tgtgtttaaa aataatttta acaaactgaa attttccgta acacttttta
      721 ttagaacaat tttttacatt tttattattt cgttttctgt attaactgta tgggttgttc
      781 atgaaagtag tctcaatgat gaatctcttt taaacacaat taaaagtgaa gattttagaa
      841 aatttatttt aaccggtgat ttcaaaaaca tattcttttt tgctattgca gccagttttg
      901 ttattaattt ttttatgcag ataaattctc tgctcggtaa aaaagttctt ttaaattata
      961 ttacaggaaa gtatcacaag ccaagaatag aattcagaac atttatgttt ttggatctca
     1021 cttcatcaac cactatagcc gaaaaactcg gaccggtaaa atatcataac tttataaata
     1081 actttttttt tgatattgat gaagccatag ttaaattcaa aggtgaaatt tatcagtatg
     1141 ttggtgatga agtcattgtc agctggattg acaatactgg atttgaaaaa aattcttgtg
     1201 tgaattgctt tcttgaaatg aaaaatatta taagcagatt atccaaaaat tatataaata
     1261 gatttggata tatacctgaa ttcaaagcag gtgttcatta cggagaagtt gtaaccggtg
     1321 agattggtga ttcgaaaaaa gagatcgttt ttcacggtga tgttcttaat acggcatcca
     1381 ggattcaggc attttctaaa actttagata aagatatatt aatatcagag gaagttcaaa
     1441 gaaagttaaa taaagaaagc aatatgagaa tagaaaatat tggaaagtat aaactaaaag
     1501 ggaaagaaat tgaactggaa ttattcagtg tagaattttt ctgatagata agtgtaaata
     1561 tcagattgag ctctgtaatt attatttgac ccggaattta tatattcgtt tttctgatct
     1621 ttgtcaatta ttctggcttt taaattatct cttatttgaa tgtctaatat atcaattaat
     1681 tccattttca gatcttcatc ataaacagga attgctgtct caattcttct attgagattt
     1741 cttttcatcc agtctgctga agaaatataa acttttttat ctcctccatt atgaaaaatg
     1801 aatacacggc tgtgttcaag atatttgtct acaatgctga taatttttat atttgaactt
     1861 tgctttttta ctcccgggat aagacaacat atacctctga caagtatgaa aatttttaca
     1921 cctgcttttg aagcttcata caatttatct atcatttttc ggtcttccag actattgagc
     1981 tttaatatta ctgaagcttc tttcccttta tctgcattct taatttcaga atcaattaat
     2041 ttcaaaaact catctttcat attaaattgc cctaccagca aatttttgaa ttcagtatca
     2101 atgattttgc cttctaagaa atcaaaaatt ttggagactt cttcagtaat atttttcgaa
     2161 gatgtaaata aaccagtatc acaataaatt cgtgaagttt tttcattgaa attacctgta
     2221 gcaaggtaac aataatttac tggtacttca ttttcaattc tggtaaccat agcaagtttt
     2281 gcatggacct ttatacccgg tatactataa agaacatgtg ctccggcttt ttgaagctca
     2341 cctgcataaa cgatattcag ttcttcatca aaccttgctt taatttcaat gaagactgta
     2401 acgcgtttac catttttaag cgcattcaat aaagagttta caattgagga attttttgca
     2461 actctgtata aagttatttt aatattttca actgtttcgt catctgcagc agcactgata
     2521 aaatctgtaa caggagaaaa agattcataa ggaaaatgaa gtatctgatc tttttctgaa
     2581 atggtctgaa agatattttt agatttataa atatatttag tctttagttc ttctttatgt
     2641 tgataaacac aatgtttgat atcaggaaaa ggaaatccaa ggaagtcctg aaaattatgg
     2701 tattta
//
LOCUS       DAORVQ010000323         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_36118_length_2676_cov_5.270126, whole genome shotgun sequence.
ACCESSION   DAORVQ010000323 DAORVQ010000000
VERSION     DAORVQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36118_length_2676_cov_5.270126"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2541
                     /locus_tag="PLX80_14605"
     CDS             <1..2541
                     /locus_tag="PLX80_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22068.1"
                     /translation="ADHFLFIKNYFSKSSKGKVNISYDLYGTVFNLPQQMQAYSPQRN
                     ENNSKLGTLFQNSWQLADAVIDFSSYDTANTAFVIFHAGVGRDVDLISIFGFDPTPYD
                     IPSVFLGLKNLQEFYGSNYEGYQTSEGIYIRNSLIIPSTELRELSLSTGDFLIELGMN
                     GIFTANFGSYLGLPDLFNTATGNTAIGRFGLMDGQSLFSYNGIFPPEPSAWEKIHLGW
                     VEPVTINSGSKNIQIPTSSKDIQRDSTIYKVLISSKEYFLIENRNRDPENDGQTIYSR
                     NRTFNDVDTYTQDIENGFYYSGGYSSLYLLNGNVTDVQTFDWSLPGLINSTNDYKGGI
                     IIWHIDENIIDAKISTNSINNDPLRRGVDVEEAKGAQEIGVTFSTPFGNVTGDGTAVD
                     YWYNGYHGVPSTIYKNEFTPTSTPNSLSYSLSNNNIFITNFGLIDTLMSLNVSIGSSQ
                     LSALNQFPKFVGIDSSISSNAIAFDLIGDNSEEIFVNSSGKIFGFSSDGNGLNGNGLL
                     FNDFGKYTPAFIETGSAKYIVGLKNDGIGFLDNTYNLTQVNFPLGAVPSAAPLINDNN
                     NFSYVGFNTGAVRRYNLDLSNVLTDSIQGNIQQFSKLRTDTFAVINKNYRYLASGNLL
                     SETSTDILTINFNNEFFINGNKLNLNYNILNVANSPTLADINSDGLQEIIFNADGFVY
                     AINSKGVLLENFPVNFNKIITSGISVADVNDDNIFDLLFVSDNGDLYAYGTDGNIVSG
                     YPVLVGPKTVSTPAIANFNDTLGIIVLCGDGYVYGFKTNTLYLANNILWKNYLSSSSL
                     SNNNYVSDNSGTATYSDKLPSSQVYNWPILFMKIQLLSDILLMEFQAV"
BASE COUNT          891 a          440 c          469 g          876 t
ORIGIN      
        1 gctgatcatt ttttattcat aaaaaactat ttctcaaaat cttcaaaggg taaagtaaat
       61 atcagttacg atctttatgg aactgtattt aatctgcctc agcaaatgca ggcatattca
      121 cctcagagaa atgaaaataa ttctaaactt ggaactctat ttcagaattc atggcagcta
      181 gcagacgcag ttatagattt ttcgtcttat gacactgcaa atactgcatt tgtaattttt
      241 cacgcaggag ttggaaggga tgtagatctt atttctatat ttggatttga tccgacacca
      301 tacgatatac cctcagtttt tttgggatta aaaaatctgc aggaatttta tggaagtaat
      361 tatgaaggat atcaaactag tgagggaatt tacattcgaa attctctaat aattccatca
      421 actgaacttc gagagctttc tttatctaca ggtgattttc taatagaact tggaatgaat
      481 ggaattttca cagcaaattt tggaagttat ctcggattac ctgatctgtt taatacagca
      541 actggaaata cagcgatcgg tagatttggg cttatggacg gtcagtccct tttcagctat
      601 aatggtattt ttccacctga gccatctgcc tgggaaaaga ttcatcttgg ctgggtagaa
      661 ccagtaacaa ttaattccgg atcaaaaaat attcagattc ctaccagctc aaaagatata
      721 caaagagatt ctacaattta taaagttctg atcagttcta aagaatattt tctaatcgaa
      781 aaccgaaaca gagatcctga aaatgacggt cagaccatat actcacgaaa cagaacattc
      841 aatgatgttg atacttacac tcaagacatt gaaaacggat tttactattc aggcggttat
      901 tcaagtctat atcttttaaa tggaaatgta actgacgttc aaacctttga ctggagtctt
      961 cccggattga ttaacagtac taatgattat aaaggcggga ttattatctg gcatattgat
     1021 gaaaatatta tagatgcaaa aatttctacc aactctatca ataatgatcc actaagaaga
     1081 ggagtggatg tagaagaagc taaaggagcg caggagatcg gtgttacatt ttcgacacct
     1141 ttcggaaatg taaccggaga tggaacagct gtggattact ggtataatgg ttatcacgga
     1201 gttccttcaa caatttataa aaatgaattt accccaactt ctactcctaa ttctctaagc
     1261 tattctcttt caaataacaa tatattcatt actaattttg gactgataga tacactcatg
     1321 agtttaaatg tttctatagg atcttcacag ttaagtgcat tgaatcagtt tccaaaattt
     1381 gtcgggatcg attcaagtat tagttccaat gctattgcgt ttgatcttat aggcgataat
     1441 agtgaagaga tatttgtcaa ttcaagtgga aaaatatttg gattctcaag tgacggtaac
     1501 ggtctgaacg gtaacggatt acttttcaac gactttggaa aatatactcc ggctttcatt
     1561 gaaacaggca gcgctaagta tattgtaggt ctcaaaaatg atggaattgg attcctggat
     1621 aatacttaca atcttacaca ggttaatttt cctttaggtg ctgttccatc tgcagctcca
     1681 cttattaatg acaataataa tttttcatac gttggattta atacaggtgc agtaagaaga
     1741 tataatttgg atctttcaaa tgttctaact gattctattc agggaaatat tcaacagttt
     1801 tcaaaactac ggacagatac ttttgcagtt ataaataaaa attatcgata tttagcttca
     1861 ggaaatctgt tgagtgaaac ttcaacggat atccttacaa taaatttcaa taatgagttt
     1921 ttcataaatg gcaataagct aaatttaaat tacaacattt taaacgtagc aaactcccct
     1981 acccttgctg acattaattc agacggactt caggaaataa tctttaatgc tgacgggttt
     2041 gtttatgcca ttaattcaaa aggagtttta ctggaaaatt tcccggttaa ctttaataaa
     2101 attattacat ccggaatttc agttgcagat gtgaatgatg ataatatatt cgatctatta
     2161 tttgtttcag ataatggaga tctctatgct tatggaactg atggaaatat tgtcagcggt
     2221 tacccggtat tagttggtcc taagaccgta tcaactcctg caattgcaaa tttcaatgac
     2281 acactgggaa ttatagtttt atgcggtgac ggatatgttt atggatttaa aacgaatact
     2341 ctctatttag caaataatat tctctggaaa aattatctca gcagcagttc tctttcaaat
     2401 aataattacg tatcagataa ttcaggaact gcaacttatt cagataaact tccttcttca
     2461 caggtttata actggccaat cctgtttatg aaaattcaac ttttatcaga tattttatta
     2521 atggaatttc aagcggtgta aaagtaaaaa tactcgatct ttccggagag ctgataacag
     2581 aattgcctgc aacttcctat tcaaatgcag ataatgaaat caaatgggac gtatcaggcg
     2641 tacagagcgg aatttattat ggaatagtag aagcac
//
LOCUS       DAORVQ010000324         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_39041_length_2562_cov_6.423614, whole genome shotgun sequence.
ACCESSION   DAORVQ010000324 DAORVQ010000000
VERSION     DAORVQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39041_length_2562_cov_6.423614"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1603
                     /locus_tag="PLX80_14610"
     CDS             302..1603
                     /locus_tag="PLX80_14610"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY22069.1"
                     /translation="MKKLFTLVLSTLVFGIFLGFSNNDNPVNRDRSNYSGTAVQVNNS
                     NTVYHGTENDNLTGEYYTDNFDGPNEVTDLVARGYVVKRNGNNPGFQGAPPWFTGFSL
                     SDGIFNSFNGPNTGYVSSNFASTSSFGTLDNWLILPKQSVMAGDSICFRARGFFGIDS
                     ITVMYSPNGATTVPPPGVSSPDWIKLGDFQAVPSGQPGFWEYKAYAAPSSGDTARFAI
                     RSFIHDAGPGAPQSDWIGIDALNLKRDDPLPVELSSFVSMISGNDVTLNWSTASETNN
                     SGFDVERNSNGVWTKIGNVTGNGTVNSAVNYSFTDRNLNSGVHSYRLKQIDFNGNFEY
                     FNLSSEVVIGVPSAFVLSQNYPNPFNPSTKIDYQLPTDGNVNISVFDNSGKEVMTLVN
                     EFKSAGYYTINFNASQLSSGIYFYKISADNFSGVKKMILVK"
     gene            1909..2562
                     /locus_tag="PLX80_14615"
     CDS             1909..2562
                     /locus_tag="PLX80_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22070.1"
                     /translation="MFIGKFYSNDKRSFKFSYSNNVKNVFRVLIITLLIFSFSNNSYS
                     QSNSGNKFVEWTTWTLFQTIPSPTFYQDRNDGNARLQFGLRWNVTPFNYSFNANKLIP
                     AAQFFKVNPVRRYGGSMEIFLQPEWATGNFQYSDFKRFNLTTGVRGFIPLEERGEYLA
                     ISLGGKYNFRKNKQDQNIGYYSVEGGIYTFFGILGLVADYNFTSQSYYNISINLKYY"
BASE COUNT          833 a          442 c          421 g          866 t
ORIGIN      
        1 ttttaaatta ataaatatta tttttcaata attttagaac tatttattta aaatttaaaa
       61 tgttttggac agcactgatt tttagataat tttgcggaaa aatttatgtg tttagtacaa
      121 taccttgggt acttatatca tactatatta tttgatttca aactaatttc aaatgtttta
      181 tgattaattg cataataaaa ctggggattt acgaataaaa atatctttga ttcctttaat
      241 ttaaaaattt aatttaaaca aactaaatca acattttttt ttcaaactta aaacaattaa
      301 aatgaaaaaa ttatttacac tcgttttatc tactttagta tttgggatat tccttggctt
      361 ttcaaacaat gacaatcctg ttaacagaga cagatccaat tattccggaa ctgcagttca
      421 ggtaaacaat tcaaatacag tttatcatgg aacagaaaat gataatctga ccggtgaata
      481 ctataccgat aattttgacg gaccgaacga agtaactgat cttgtagcaa gaggttatgt
      541 agtaaaaaga aacggtaaca acccgggatt tcaaggagca ccgccttggt ttacaggttt
      601 ctctttaagt gacggaatct ttaattcttt taacggaccg aacactggtt acgtgtcttc
      661 aaactttgct tcaacttctt ctttcggaac tttggataac tggttaattc ttccaaagca
      721 aagtgtaatg gcaggagact ccatctgttt ccgggcacgc ggattctttg gtatagattc
      781 aatcactgtc atgtacagtc caaacggagc aactacagta cctcctcccg gtgtatccag
      841 tcctgactgg ataaagcttg gagattttca ggcagtacct tcaggtcagc ccggattctg
      901 ggaatacaaa gcctatgctg ctccaagctc aggtgacact gcaagatttg cgatcagaag
      961 tttcatacat gatgcaggtc ccggcgctcc tcaaagtgac tggatcggaa tcgacgctct
     1021 taatttaaaa agagatgatc cgcttcctgt tgaactatca tcttttgtat caatgatctc
     1081 tggaaatgat gtcacgctga actggtctac tgcatcagaa accaataact caggttttga
     1141 tgtcgaaaga aacagtaacg gtgtctggac taagatcgga aatgtaaccg gtaacggaac
     1201 tgtaaactct gctgtaaatt attcttttac tgacagaaat ctgaattcag gggttcacag
     1261 ctacagactg aaacagatcg actttaatgg taactttgaa tatttcaatc tgagcagtga
     1321 agttgtgatc ggtgttcctt ctgcatttgt attatcgcaa aactatccga atccatttaa
     1381 tccatctaca aagattgatt atcagttacc aactgatggt aatgtcaaca tttccgtatt
     1441 tgataactca ggaaaagaag tgatgacttt agttaatgaa tttaaatcag caggttatta
     1501 tacaatcaat tttaatgcat ctcaattatc aagcggtatt tatttctata agatctctgc
     1561 agataatttc tcaggtgtta agaaaatgat ccttgtaaaa taagctgctc gaaattcgtt
     1621 ttagttttaa atttatcagc aggtcagtat taatttactg acctgttttt tttctgccgc
     1681 aaagtcacca aagcacaaat tcaaatgatc tgctcttgtt aaaatactgt ttgaaaaatg
     1741 caaacctgcc tttattcctg tcagcttaca aattttattc ccgctggaaa atatatactc
     1801 aatgtaatga ctctcaaaat tctttatcat caaactcatt tttttacttt gattaacata
     1861 taatttattt atattgataa ataaaaattg cctgttcaaa ctttataaat gttcatcgga
     1921 aagttttact caaatgataa acgcagcttt aaattctcat attcaaataa cgtgaaaaat
     1981 gtattcagag tattaattat tactctctta atattttcat tttcaaataa ctcttattcc
     2041 caaagcaatt ccggaaataa gtttgttgaa tggactacct ggactttatt tcagacaata
     2101 ccatcaccga ctttttatca ggatagaaat gacggcaatg caagattaca gtttggattg
     2161 agatggaatg ttactccttt taattattca tttaatgcaa acaaacttat tcctgctgcg
     2221 caattcttta aagttaatcc cgtgagaaga tacggaggct cgatggagat ctttttacaa
     2281 ccggaatggg caaccggtaa ttttcagtat tcggatttta aaagatttaa tcttactacc
     2341 ggtgtcagag gatttattcc acttgaggaa agaggtgaat atctcgcaat ttcccttggt
     2401 ggtaaatata attttcgaaa aaataaacag gatcagaata ttggatacta ttcagtcgaa
     2461 ggcggaatat atactttctt cggaattctc ggtctggtcg cagattacaa ttttacttca
     2521 caaagctatt acaatatttc catcaacttg aaatattatt ga
//
LOCUS       DAORVQ010000325         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_40551_length_2508_cov_4.640440, whole genome shotgun sequence.
ACCESSION   DAORVQ010000325 DAORVQ010000000
VERSION     DAORVQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40551_length_2508_cov_4.640440"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1408)
                     /gene="ahcY"
                     /locus_tag="PLX80_14620"
     CDS             complement(83..1408)
                     /gene="ahcY"
                     /locus_tag="PLX80_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQY22071.1"
                     /translation="MSTSTIDKTEVLPYKVKDISLAEFGRKEIRLAEAEMPGLMAIRE
                     EYKSTKPLKGARIAGCLHMTIQTAVLIETLIELGAEVTWSSCNIFSTQDHAAAAIAAS
                     GIPVYAWKGMNEEEFNWCIEQTLFFGADRQPLNMILDDGGDLTNMVFDKYPELIAGIK
                     GLSEETTTGVLRLYERMKKGTLSVPAINVNDSVTKSKFDNKYGCKESLVDAIRRATDV
                     MMAGKVAVVAGYGDVGKGSAQSLRGAGARVMVTEIDPICALQAAMDGYQVVTMDEGSK
                     IADIVVTATGNYNIITERHFKSMKDKAIVCNIGHFDNEIDMAWLNKNYGDTKDTIKPQ
                     VDIYNIDGHEVIILAEGRLVNLGCAMGHPSFVMSNSFTNQVLAQIELWTNSQNYENKV
                     YVLPKHLDEKVARLHLEKVGATLETLTKEQADYIGVEVEGPYKSETYRY"
     gene            complement(1625..2119)
                     /locus_tag="PLX80_14625"
     CDS             complement(1625..2119)
                     /locus_tag="PLX80_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018884014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQY22072.1"
                     /translation="MNIGFLVVDMQKIFLDELREKINVQRAVEHINYVSEMLRSKGHC
                     IVHIQDIEDENENNSHQIDFIPEIVIAESDLRLKKIKSNSFMDTDLEKLLKDKEVELI
                     IVSGFSAEHCVLFTYNGALERGFNSVILQNGILSANISAVSETQRDRNLISYPVVEYL
                     TKSA"
     gene            complement(2239..>2508)
                     /locus_tag="PLX80_14630"
     CDS             complement(2239..>2508)
                     /locus_tag="PLX80_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQY22073.1"
                     /translation="MTNVEDVTGDDLNDLKSNTPSLESLTRTELKILKFISKNKTSKE
                     IAKDLFLSPRTIENHRTNISNKLNLKGSHSLIKFAIENKSVLSDL"
BASE COUNT          731 a          489 c          344 g          944 t
ORIGIN      
        1 ctattctata aaaataaaaa atcagataaa gtttttaaaa ccttacctga tttatttttt
       61 attaaaaatt taatatttaa tattagtagc ggtatgtttc cgacttataa ggtccttcaa
      121 cctcaacacc aatataatca gcctgctctt ttgttaatgt ttcaagagtc gcaccgactt
      181 tttcaagatg aagtcttgct actttttcat ccagatgctt cggaagtaca tatactttgt
      241 tctcataatt ctgtgaattt gtccaaagtt cgatctgtgc aagcacctga tttgtaaatg
      301 aatttgacat tacaaatgaa ggatgtccca ttgcgcatcc aagatttaca agtcttcctt
      361 cggcaaggat gatcacttca tgaccgtcaa tgttataaat atctacctga ggcttaattg
      421 tatcttttgt atctccgtaa tttttattca gccatgccat gtcgatctca ttgtcaaaat
      481 gaccaatgtt acaaactatg gctttgtctt tcatgctttt gaaatgtctt tcagtaataa
      541 tattataatt tcctgttgca gttactacta tatctgcgat ctttgaacct tcgtccattg
      601 tcacaacctg atatccatcc atcgctgcct gaagtgcgca gatcggatcg atctcagtta
      661 ccattactct tgctccggca cctctcagtg actgagctga accttttcca acatctccgt
      721 aaccagccac tactgctact ttccctgcca tcattacatc tgtcgctctt ctgatcgcgt
      781 ctaccaatga ttctttacat ccgtatttat tgtcaaattt tgatttggta actgaatcat
      841 ttacattgat cgccggaact gaaagtgttc cttttttcat tctctcatat aatctcaaaa
      901 ctcctgttgt agtctcttct gataaccctt ttattcctgc aattagctca ggatatttat
      961 caaaaaccat atttgtaaga tcaccgccgt catcgaggat catatttaac ggctgtctgt
     1021 cagctccgaa aaataaagtc tgctcaatgc accagttgaa ttcttcctcg ttcattcctt
     1081 tccaggcata aaccggaatt cctgaagctg caatcgctgc tgctgcgtga tcctgtgttg
     1141 agaaaatatt acatgaagac catgtaactt cagctccaag ttcaatcagt gtttctatca
     1201 atactgctgt ctgaattgtc atatgaagac atcctgcgat cctcgctcct tttaacggct
     1261 tagttgattt atattcttct ctaatagcca ttaatcccgg catttctgct tctgccagtc
     1321 tgatctcttt tctgccaaat tctgcaagtg aaatatcttt taccttatat ggtaatactt
     1381 ctgtcttgtc tattgttgat gtactcaagg ttttactcct ttttgatatt taattattaa
     1441 ttggatttct ttttttggac tgtaaagata acacatataa ttttttaaat aaatcgataa
     1501 attagtatta tttttaaaaa taggtttata caaatataaa ttattttaag aatttcagac
     1561 ttttcgaaga tattatataa atccaatctc cttttaataa atactgaagg atttaatgtg
     1621 ataattatgc agatttggtc aaatactcta ctacaggata tgatattaaa tttctgtctc
     1681 tctgagtctc actcactgct gaaatgttag cgcttaatat tccgttttga agaattactg
     1741 aattaaatcc cctctccaat gctccgttat atgtaaacaa aacacaatgc tcggctgaga
     1801 atccggaaac aattattaat tcaacctctt tgtctttcaa taacttttca agatcagtat
     1861 ccatgaatga attcgattta atttttttaa gcctcagatc actctcagca attacaattt
     1921 caggaatgaa atcaatctga tgggaattat tttcattttc atcttctata tcctgaatgt
     1981 gaactataca atgtcccttt gacctaagca tctccgatac ataattaata tgttccactg
     2041 ctctctgtac attaattttt tctctgagtt catcaagaaa gatcttctgc atatctacaa
     2101 ctaaaaatcc aatattcata ttaatttatt taattatttt tatttaaaac ttttaataga
     2161 ttcaactaaa aacgttaata tgcgggaata aaaaatctaa taaatctaat aaatctaata
     2221 aatctaataa atcaaatttt agagatctga aagaaccgac ttattttcta ttgcaaattt
     2281 tataagactg tgacttcctt tgaggttgag tttattactt atgtttgttc ggtgattttc
     2341 aattgtcctc ggactgagaa aaaggtcttt agcaatttct ttgcttgttt tatttttaga
     2401 aatgaatttt agtattttca gttctgtccg tgttaaactt tcaagtgatg gtgtattgct
     2461 ttttaaatca ttcaggtcat cacctgtcac atcttcaaca tttgtcat
//
LOCUS       DAORVQ010000326         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_41091_length_2491_cov_5.248358, whole genome shotgun sequence.
ACCESSION   DAORVQ010000326 DAORVQ010000000
VERSION     DAORVQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41091_length_2491_cov_5.248358"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLX80_14635"
     CDS             <1..613
                     /locus_tag="PLX80_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQY22074.1"
                     /translation="GSEEKCTGDPARRLGNEFLAQSFIQQNAETLKKFNVKKVVTACP
                     HCMHTLKNEYGQFGVDLEVTHHTEFIDDLVKEKKLIPVKENNETVTYHDSCYLGRYND
                     IYEQPRESIKSIPGINIVEMARSKDKGFCCGAGGGRMFLEETEGKRVNIERTEEALET
                     GAKTIVSACPFCMTMLTDGVKEKEKSEEVKIKDVAELVLESLE"
     gene            674..1927
                     /gene="metK"
                     /locus_tag="PLX80_14640"
     CDS             674..1927
                     /gene="metK"
                     /locus_tag="PLX80_14640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQY22075.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDNFLAFDPNAKVACETL
                     VTTGQVVLAGEVNSKIYLDVQSIARGVIEKIGYTKSEYKFDAESCGVFSGIHEQSKDI
                     NQGVVRSKKEDQGAGDQGMMFGYATNETQNYMPLPLDLSHILLYELAEIRRENKVMKY
                     LRPDSKSQVTIEYDDNNKPKSIKTIVVSTQHDEFSSEKAMRSKIEADVKNILIPRVKS
                     KYKNYGQLLNGNIQYHVNPTGNFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGK
                     DPSKVDRSAAYATRHIAKNLVAAGVCDEVLVQVSYAIGVAKPMNIFVNTYGTGKINKK
                     DSEISDVVEKLFDMRPYFIEQRLKLRNPIYSETAAYGHMGRKNEVVTKTFKTPDGKDK
                     TVKVELFTWEKLDYVGKIKKAFGIK"
     gene            1983..>2491
                     /locus_tag="PLX80_14645"
     CDS             1983..>2491
                     /locus_tag="PLX80_14645"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HQY22076.1"
                     /translation="MNNFIHGFFYPFKCINLFFKYPKLILYSIVPMVINLIIYGTIFF
                     YTYNWLIGSSSDVLNDNVPENFLVLIFGYFLKIFSFFLVLLICYFTFVIFGGLVSAPF
                     NERMSKLIEENEFGIHLESGLSFSSETYFSIKEELKKILFYLLFIVPLFLINFIPMIG
                     NTISFVFGTL"
BASE COUNT          892 a          355 c          487 g          757 t
ORIGIN      
        1 tggaagtgaa gaaaaatgta caggtgatcc tgcaagaaga cttggcaatg aatttcttgc
       61 acaatctttt attcagcaga atgcagagac tcttaagaaa ttcaatgtaa aaaaagtagt
      121 tacagcctgt cctcattgta tgcatacttt aaaaaatgaa tacggacaat tcggtgtaga
      181 tcttgaggtg acacatcaca cagaatttat tgacgacctt gtgaaagaga aaaaattaat
      241 tcctgtaaag gaaaataatg aaacagttac atatcatgat tcctgttacc ttggcagata
      301 caatgatatt tatgaacagc cgcgtgaatc tataaaaagc attccgggaa ttaatatagt
      361 agagatggca agatctaagg ataaaggatt ctgctgtgga gcaggtggcg gaaggatgtt
      421 ccttgaagag actgaaggta aaagagtaaa tatagaaaga actgaagaag ctcttgaaac
      481 cggtgcgaaa actattgtat cagcctgtcc gttttgcatg actatgttaa cagacggtgt
      541 aaaggaaaag gaaaagagtg aagaagtaaa aataaaagac gttgcagagt tggtacttga
      601 aagccttgaa taaaaataat ttttcaaata aaacaaaatt aataattaaa tatataataa
      661 ggagaaataa ttaatgtcat atctatttac ttctgaatca gtttcagaag gacatccgga
      721 taaagtagca gatcagattt cagatgcgtt gatcgataat tttctagcgt ttgatccaaa
      781 tgcaaaagtt gcatgtgaga ctttggtaac aacaggtcag gtagttttag ccggtgaagt
      841 aaactcaaag atctatcttg acgtgcaaag cattgcaaga ggagtaattg aaaagatcgg
      901 ttatacaaaa agcgaatata aatttgatgc agaatcctgc ggtgtttttt caggtattca
      961 cgaacaatca aaagatatta atcagggagt tgtaaggagt aagaaagaag atcagggagc
     1021 aggtgatcag ggaatgatgt ttggctatgc aactaacgaa acccaaaatt atatgcctct
     1081 gcctttggat ctttcacata tattacttta tgaactagct gagatcagaa gagaaaataa
     1141 agtaatgaaa tatctcagac ctgattctaa atcgcaggtt acgattgaat atgatgacaa
     1201 caataaacca aagagcatta agacaattgt tgtatctact cagcatgacg agtttagcag
     1261 tgaaaaagca atgagaagta aaatagaagc tgatgtaaag aacattctga ttccgagagt
     1321 aaaatcaaag tataagaatt atggtcagct tttaaatggt aacattcaat atcatgtaaa
     1381 cccgaccgga aattttgtta ttggcggacc acacggagat accggactta caggtcggaa
     1441 gatcatagtt gatacttacg gcggaaaagg tgcacacgga ggaggagcat tctcaggaaa
     1501 agatccttcc aaagtggaca ggtcagctgc ttatgctacc agacacattg caaaaaattt
     1561 agtagctgca ggtgtttgtg atgaagttct tgttcaggtg tcttatgcaa tcggtgttgc
     1621 aaaaccaatg aatatctttg ttaatactta tggtaccggc aagatcaata aaaaagacag
     1681 tgagatttct gatgtagtgg aaaaattatt tgacatgaga ccatatttta ttgagcaaag
     1741 acttaaacta agaaatccga tttacagtga aacagcagct tacggacata tgggaagaaa
     1801 gaatgaagtt gtaaccaaga cattcaagac acctgacggt aaagataaaa ctgtaaaggt
     1861 cgaattgttt acatgggaaa aacttgatta cgttggcaaa attaaaaaag cttttggtat
     1921 taagtaatct gtattttttt tgttttaaaa tattaacagt ttaaattttg atatattaaa
     1981 taatgaataa ttttatacac ggtttttttt atccgtttaa atgtataaac ttatttttca
     2041 aatatcccaa gttaatttta tattctattg ttccgatggt gatcaatctg atcatctacg
     2101 gaacaatttt tttttatacc tacaactggc ttatcggcag ctcttcggat gttctgaatg
     2161 acaatgtacc tgaaaatttt ctagtattaa tattcggtta tttccttaaa atattctcgt
     2221 tctttttagt tctactcata tgctatttta cttttgtaat attcggcgga cttgtctcag
     2281 ccccatttaa tgaaaggatg tcaaagctta ttgaggaaaa tgaatttgga attcatctgg
     2341 aaagcggtct ttctttttca tcagaaactt atttcagtat taaagaagaa ttaaaaaaga
     2401 tattatttta tttacttttc atagtcccgc tttttttaat aaactttata ccaatgatcg
     2461 gaaatacaat ttcttttgta ttcggaactc t
//
LOCUS       DAORVQ010000327         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_41192_length_2487_cov_4.159128, whole genome shotgun sequence.
ACCESSION   DAORVQ010000327 DAORVQ010000000
VERSION     DAORVQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41192_length_2487_cov_4.159128"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLX80_14650"
     CDS             <1..349
                     /locus_tag="PLX80_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22077.1"
                     /translation="TKEFSFGKFPFGRAAVEYSLIFRETHVNQLRFSYNFDIPLVSGD
                     FAVIMFSAGGGYFTDFDKKGYFPQTSVALLLPASETFTANVYFKTRYTIMDDKSESNI
                     FDITFGLSAAIYF"
     gene            351..1133
                     /locus_tag="PLX80_14655"
     CDS             351..1133
                     /locus_tag="PLX80_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HQY22078.1"
                     /translation="MKSEQNKNINYKRYERQTILKEIGESGQKELMKASVLVIGAGGI
                     GCPLLSYLSATGIGKIGIADYDKVELSNLHRQVLYNEDQTGRSKALSAADNLLKMNSE
                     TELKVFETFIGKSNGADIIKEFDIIADATDNFKSRYIINDLCVINNKPFVWGAVNKFE
                     LQVAVFNYNGGATFRCAYPENFSAVNIQNCEEQGVIGPVPGLTGMLMAGEIIKILTGS
                     GEILNDQILIGNILKSDFRKIKLIRDPEQIEKAFEGIRKMDK"
     gene            complement(1322..2182)
                     /gene="pucL"
                     /locus_tag="PLX80_14660"
     CDS             complement(1322..2182)
                     /gene="pucL"
                     /locus_tag="PLX80_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HQY22079.1"
                     /translation="MSSKLKSNNYGKSNVRILKVKRDNSIHNIVEMKINVQFKGDFDN
                     VHLNGDNSNVLPTDTIKNTLYILAKENDINSVEEFIIYAAQYFYNNNEQISEVKINAE
                     EKIWKRINVSSDDEINSAFHDHSFICPGLEVRTCKVKVGNDKFSIRSGLKDLLVLKTT
                     CSGFEGYIKDKYTTLPETDDRVFSTSIKAEWKFKNSENDYIKIFNEVRRIIINTFADH
                     DSLSVQHTLYECGKKVVEQCPAIKQIFLSMPNKHYLKFDLDRFGIENNNEIFIPTDEP
                     FGLIEGVIKN"
BASE COUNT          846 a          348 c          400 g          893 t
ORIGIN      
        1 tacaaaggaa ttttcttttg gtaaatttcc attcggaaga gctgcggtag aatattcttt
       61 gatattcaga gagactcatg tcaatcaatt gagattttca tataattttg atataccatt
      121 agtgtcagga gactttgcgg taataatgtt ttctgcaggc ggcggatact ttacagattt
      181 tgataagaaa ggatattttc cgcagacgtc cgtagcttta ctgctaccag cttcggagac
      241 atttacagca aatgtttatt ttaaaacaag gtacactata atggatgata aatcagaatc
      301 aaatattttt gatataactt ttggtttaag tgcagcaatt tatttttagt ttgaaaagcg
      361 aacaaaataa aaatattaat tataaaagat acgaaagaca gacgattcta aaagagatag
      421 gtgaatcagg tcaaaaagaa ttaatgaaag catctgtttt agtgatcggt gccgggggaa
      481 tagggtgtcc gcttctttca tatctctctg ctacaggtat cggtaaaatt ggaattgcag
      541 attatgataa agtagaatta tcaaatttac acagacaggt cttatacaat gaagatcaga
      601 caggcagatc aaaagcattg tcagcagcag ataatctttt gaaaatgaat tcagaaactg
      661 aattaaaagt ttttgaaact ttcataggta agtcaaatgg tgctgatata attaaagaat
      721 ttgatatcat cgctgatgct actgataatt ttaaatcccg ttacataatt aatgatcttt
      781 gcgtgataaa taataaacca tttgtatggg gtgctgtaaa taaattcgaa ttacaggttg
      841 cagtttttaa ttataatgga ggagctactt tcagatgtgc ttatccggaa aatttctcag
      901 cagtaaatat tcagaattgt gaagagcagg gtgtgatcgg tcctgtgccc ggattgaccg
      961 gaatgcttat ggcaggagaa attataaaaa tattaacagg cagtggtgaa atattaaatg
     1021 atcagatttt gattggaaat attttgaaat cagattttag aaagattaaa ctaataagag
     1081 atccggagca gattgaaaaa gcatttgaag gaatcagaaa aatggataaa tgaaattatt
     1141 aaattcggca acaccaccct tcgcttcccc ttttttaaag gggaattact tttgaattat
     1201 actgaaacgt gcttcttgaa atttagactt cggttctgaa aaattcagca aaaatattca
     1261 taccattttg aggttaatct aagttgtaac agactaattc ttaattccta atttttaatt
     1321 cttaattttt tataactcct tctattaatc caaacggttc atcagtcgga ataaatatct
     1381 cattattatt ttcaatacca aacctatcaa gatcaaactt caggtaatgt ttatttggca
     1441 tagataaaaa tatttgtttt attgccgggc attgttcaac aactttttta ccgcattcat
     1501 aaagagtgtg ctgtacagaa agactgtcgt gatctgcaaa agtattaatt ataatccttc
     1561 ttacttcatt aaagattttt atataatcat tttcagaatt tttaaatttc cattcagctt
     1621 ttatacttgt cgaaaaaact ctgtcatctg tctcaggaag tgtagtgtat ttatccttta
     1681 tataaccttc aaaacctgag caggtagttt tgagaactaa caaatctttt aaaccagatc
     1741 tgatactgaa tttatcattg ccaactttta ctttacatgt cctgacttca agacctggac
     1801 agatgaagga gtgatcgtga aacgcagaat ttatttcatc gtcagaagaa acatttattc
     1861 ttttccagat cttctcttcg gcatttattt ttacttctga aatctgttca ttattattat
     1921 aaaaatattg agctgcataa ataataaatt cctcaacaga attaatatca ttttcttttg
     1981 caagtatata taatgtattc ttgattgtat ctgtaggaag gacattgctg ttgtctccat
     2041 tcaaatgaac attgtcaaag tcacctttga actgtacatt tatcttcatt tcaacaatat
     2101 tgtgaatgct gttatcacgt ttaactttta agatcctgac attggatttt ccgtagttgt
     2161 tactttttaa ttttgatgac ataataattt aaattttaaa taatataaca gttagaattt
     2221 ttttaatata gagtgaaaat ttttttgctt taagcttaca aaataaattt attaaattat
     2281 tttatggagt tatataaata aagccttttg tctatttcaa ttgtaaataa ttactaaatt
     2341 tacttatacc ttcttctaat attagattgc tgcattcgtg tgaatgacaa tttttgcagc
     2401 attcgggaaa gtaagaatct gtaaaaaaaa aattctaatt atttttcttt gtaagtaata
     2461 tagcactata atttgtattc acggatt
//
LOCUS       DAORVQ010000328         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_41474_length_2477_cov_3.943435, whole genome shotgun sequence.
ACCESSION   DAORVQ010000328 DAORVQ010000000
VERSION     DAORVQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41474_length_2477_cov_3.943435"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..782)
                     /gene="rlmB"
                     /locus_tag="PLX80_14665"
     CDS             complement(36..782)
                     /gene="rlmB"
                     /locus_tag="PLX80_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQY22080.1"
                     /translation="MLVIGKNPVLETLKSNPDAFNKIVLLKKLKPENKLKSIVTIAEK
                     EKINVVYLNYTDFKKFFDNKSKDEGVDQGVIGFIKDYQYVPIREMVEANKKITNPLIL
                     VLDSITDPHNLGAIARSAVCLGADGIVIPRHNAAEVNHTVLKTSSGAVNYISIAKETN
                     LSNTLLFLKNNGYWIAGTDLNSSKSIFETDLKLPLALVIGSEGSGIRKNLLDKCDLKI
                     RIPMAGKIDSLNASVSAGVFLYVIFRHKNS"
     gene            875..1603
                     /gene="rsmI"
                     /locus_tag="PLX80_14670"
     CDS             875..1603
                     /gene="rsmI"
                     /locus_tag="PLX80_14670"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQY22081.1"
                     /translation="MNERLSAVYNNFKEKFDLQNFHKGVLYLVSTPIGNIEDISFRAI
                     QILENADFIACEDTRKTNFLLTQYGIRNKTVSYYSHVESSKSEFIIDEIKSGKTVALV
                     SDAGTPGISDPGNLLVSKCIEENIEIYSVPGCSSLIHSLVLSGIPTGKFYFQGFLPHK
                     KGRQTALKELSEIKMTLIIFESPFRIYKALNEIHDHLGNKEIAVCRELTKKFEQTIRG
                     RVKDVLSKQFKTKGEFVIIVNNSK"
     gene            1610..>2477
                     /gene="gltX"
                     /locus_tag="PLX80_14675"
     CDS             1610..>2477
                     /gene="gltX"
                     /locus_tag="PLX80_14675"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQY22082.1"
                     /translation="MNNNNVRVRFAPSPTGYVHIGSVRTALFNYLYARNQNGKNILRI
                     EDTDQTRKVEGAVENLVDSLALLGIDFDEGPGKDGEAGPYFQSERLEIYKKYCDELLE
                     KKAAYYAFETSEELDEMRKMQQLEGRQTMYDGRARDLTDEEVKANLDKGMPYVIRLKV
                     PRDEEVRFNDVIKGNIKIETNIIDDQILLKSDGFPTYHLANVVDDHLMGITHIIRGEE
                     WVTSVPKHILIYKALGWEVPAMVHVPLILNPDKSKLSKRQGDVAVEDFLKKGYLKEAL
                     INFIAMLGWNPGE"
BASE COUNT          869 a          386 c          443 g          779 t
ORIGIN      
        1 ataaaatacc aaccccaaaa aaaagaacaa gcggatcatg aatttttatg tctgaaaatt
       61 acataaagaa aaactcccgc cgatacagat gcattaagtg aatcgatctt accagccatc
      121 gggattctga tttttaaatc acatttgtca agtaaatttt ttctgatacc tgaaccttcg
      181 cttcctatta ccaatgccaa aggaagtttc agatcagttt caaagattga tttggatgaa
      241 ttcagatcag tacctgcgat ccaataaccg ttgttcttta agaaaagaag tgtatttgat
      301 aagttagttt ctttagcaat gcttatataa tttactgctc ccgaagatgt tttcaataca
      361 gtatgattta cttctgctgc attatgacgt gggatgacaa tcccgtctgc accaagacat
      421 actgctgacc ttgcgatagc accgagatta tgaggatctg tgatactgtc gaggacaagt
      481 ataagaggat ttgttatttt cttatttgcc tctaccattt ctctgatcgg tacatactga
      541 taatctttta taaaaccgat aactccctga tctacgcctt catctttgct tttattatca
      601 aagaattttt taaagtctgt atagtttaaa tagactacat ttattttttc tttttctgca
      661 attgttacta tgctcttcag tttattttca ggctttaact ttttcaaaag tactattttg
      721 ttaaaagcat caggattaga tttcagagtt tcgagtactg gatttttacc gataactaac
      781 atttatatat aaatttaaaa tggtttttta attaagtatt ttaaatataa ttataatata
      841 ccgtaatata tcagcaaatt attattcata gtatttgaac gaaagactat cagccgttta
      901 caataatttc aaagaaaaat ttgatttaca gaattttcac aaaggagttt tgtatcttgt
      961 ttctacccca atcggaaata tagaggacat ttcttttcgg gcaattcaaa tactggaaaa
     1021 tgctgacttt atagcttgtg aagatacaag gaaaacaaat ttcctgctga ctcaatatgg
     1081 aattagaaat aagacagtta gctattattc acatgtggaa tcaagtaaat ctgaatttat
     1141 cattgatgaa ataaaaagcg gtaaaactgt tgcattggtt tcagatgcag gtacacccgg
     1201 tatttctgat cctggtaatt tacttgtttc aaaatgtata gaagaaaata ttgaaattta
     1261 ttctgtaccc ggctgcagct cgctgataca ttcacttgta ttatccggaa taccaacagg
     1321 taaattttat tttcagggat ttctaccgca caagaaagga agacagactg cattaaaaga
     1381 attatcagaa ataaaaatga cgctgatcat tttcgaatct ccgttcagaa tttataaagc
     1441 attgaatgaa attcatgatc atcttggaaa taaagaaata gctgtatgcc gtgaacttac
     1501 gaaaaaattt gaacagacca tcagaggcag agtgaaagat gtactttcta aacaatttaa
     1561 aacaaaaggt gaatttgtaa ttatagtaaa taacagtaaa taaaatttaa tgaataataa
     1621 taatgtaaga gttagatttg ctccatcccc tacaggttat gtacatattg gctctgtaag
     1681 gactgcactt tttaattatc tctatgcgcg caatcagaat ggcaaaaata ttttaagaat
     1741 agaagataca gatcagacaa gaaaggtcga aggtgcagta gaaaatcttg tagattcact
     1801 tgcacttctt ggaatagatt ttgatgaggg tccgggtaaa gacggtgaag caggaccata
     1861 tttccaatca gaaagattag aaatttataa aaaatactgc gatgaacttt tagaaaaaaa
     1921 ggcagcttac tatgcttttg aaacttctga agagcttgat gaaatgagaa agatgcagca
     1981 gctcgaaggc agacagacta tgtatgacgg cagagcgcgt gatcttactg atgaagaagt
     2041 aaaagctaat cttgataaag gaatgcctta tgtgatcaga ctaaaagttc cacgtgacga
     2101 agaagtcaga tttaatgatg taataaaagg aaatataaaa attgaaacca atattatcga
     2161 tgatcagata cttttaaaat cagacggctt tccgacatat catcttgcta atgtagtgga
     2221 cgaccatctt atgggaatca cacatattat cagaggtgaa gaatgggtta cttcagtacc
     2281 gaaacacatt ttaatctaca aagctcttgg atgggaagta ccggcaatgg tgcatgttcc
     2341 gctgatactg aatcctgata aatcaaaact cagcaagaga cagggagatg tcgcagttga
     2401 agatttttta aaaaagggat atttaaaaga agcattgatc aactttattg caatgcttgg
     2461 atggaatccg ggagaag
//
LOCUS       DAORVQ010000329         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_41497_length_2476_cov_4.606774, whole genome shotgun sequence.
ACCESSION   DAORVQ010000329 DAORVQ010000000
VERSION     DAORVQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41497_length_2476_cov_4.606774"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1736
                     /locus_tag="PLX80_14680"
     CDS             279..1736
                     /locus_tag="PLX80_14680"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQY22083.1"
                     /translation="MYLKKIFTLLFIFTIYGNLTFAQPFELQNAFPNTTFDNPLYVTH
                     SNDQTNRVFIVEKTGRIKVLPNDSLTSINKTYLDISNKIINGSERGLLGVAFHPNYSS
                     NGYLFVNYTRSGDGATVVSRFSVSPSDPDKADSLSEFILLTIAQPYSNHNGGMIFFGM
                     DGYLHIGTGDGGSGGDPGNRAQTLTELLGKILRINVDTTTALTNYGIPSSNIFAGGGG
                     RAEIFTIGMRNPWRMSVDPVTGEIWCGDVGQDAWEEIDIIENGKNYGWRCYEGNNPFN
                     TSGCSTPSTYTFPVKVYQNVGSDCSVAGGYVYRGQRRPELYGRYIYGDYCSRKVWKLK
                     YENGVVTEDSQILTAAGSILSWGTDQNNELYMCSVNGIVYRFNKSDLVGVNNNNLETP
                     NTFILEQNFPNPFNPETSIKYYLPEISNVNLVVYDANGKIISNLVNTKQLSGSYNVKW
                     NGRDALGNNLPSGVYFYNLTAGNNFSQSKKMLLIK"
     gene            complement(1985..2314)
                     /locus_tag="PLX80_14685"
     CDS             complement(1985..2314)
                     /locus_tag="PLX80_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22084.1"
                     /translation="MSSSIEAALHLGARYSGSQREKYYTVINSLYPDRYIFNIWYKVT
                     ELMDKDFLRTLLIEKNKFFFDCKSTIELNDFILFISQLTGSENITYKEVYSNKREEKI
                     YEIELNP"
BASE COUNT          851 a          362 c          410 g          853 t
ORIGIN      
        1 gacacaaacc gttaaaacgg tttatcatcc gacacaaacc gttaaaacgg tttatcatcc
       61 gacacaaacc gttaaaacgg tttatacaca taaaaaaatc actgctgtga aatttttaat
      121 aactgttaaa tgtatatcat tatgtcaatt atattgtgaa tagttattaa ctattttatt
      181 caattacaaa tcaatattat aacacttaaa tttaaattat taactattgt ccggtatatt
      241 ctattaaata cgtccggaca tttaacccag gagataaaat gtatctgaaa aaaatattta
      301 ctttgttatt tatttttacg atttatggaa atttaacttt tgctcaaccc tttgaattac
      361 aaaatgcttt tcccaataca actttcgata atcctctgta tgttactcat tcaaatgatc
      421 agactaacag agttttcata gtagaaaaaa ccggaagaat aaaagtttta ccaaatgatt
      481 ccttaacttc aatcaacaaa acctatctgg atatcagtaa taaaattatt aacggcagtg
      541 aaagaggctt gcttggtgtg gcttttcacc cgaattattc ttctaatgga tatctttttg
      601 ttaactatac aagatctggt gatggtgcta ctgttgtttc ccgattcagt gtaagtcctt
      661 ctgatccgga taaagcagat tctttaagtg aattcatact tctaacaatt gctcagccat
      721 attcaaatca taatggaggt atgatctttt ttggaatgga tggttatctt catattggta
      781 ccggtgatgg tggcagtggt ggtgacccgg gaaacagagc gcagacatta acagaattac
      841 taggaaagat tcttagaata aatgtagata ccacaaccgc tcttacaaat tatggaatcc
      901 catcctcaaa catatttgct ggtggaggtg gtagagctga aatatttact attggaatga
      961 gaaatccatg gagaatgagt gtagatccgg ttacaggtga aatttggtgc ggcgatgtag
     1021 gtcaggatgc ttgggaagaa attgatatca tagaaaatgg taaaaattat ggatggagat
     1081 gctacgaagg aaacaatcca tttaatactt caggttgctc tactccatca acctatacct
     1141 ttcctgtcaa agtttaccaa aatgtcggtt cagattgttc tgttgccgga ggatatgttt
     1201 atagaggaca aagaagacct gaactttatg gcagatatat ttacggagat tattgtagta
     1261 gaaaagtttg gaaacttaaa tatgaaaatg gtgtagtgac tgaagattca cagatcttaa
     1321 ctgcagcagg aagtatcctt tcctggggca ctgatcaaaa taatgaattg tatatgtgca
     1381 gtgtaaacgg aattgtctat agattcaata aaagtgatct ggttggtgta aataataata
     1441 acttagaaac cccaaatact tttatactcg aacagaattt tccaaatccc tttaatcctg
     1501 aaacttcaat caaatattat ttacctgaga tttcaaatgt aaatctggtt gtttatgacg
     1561 ctaatggtaa aattatttct aatcttgtaa atactaaaca actttcaggt tcttataatg
     1621 taaagtggaa cggtagagac gctttaggta ataacctccc gagcggtgtg tatttttata
     1681 acctgacagc gggaaataat ttcagtcaga gtaagaaaat gcttttgatc aaataatata
     1741 aaattgtttg taatttaatt ccggattttt ctgagcgtaa aatattaaat ccgtcagagg
     1801 ttttactgca tctgcagtta ctccggcgga tttttatttt atatcaattg tcttctgata
     1861 taatagttta tataaaaata tttaatattt tttttacgaa tctcagaatt tatgaaatgc
     1921 caaatccggc tgaacagatt tttaaaatat gaagattaaa ctgtaatgat ttgtgaaaaa
     1981 ttttttatgg atttaattct atttcgtata ttttttcctc tcttttattt gaataaactt
     2041 ctttgtaagt aatattctcc gatccggtta actgacttat aaatagtata aaatcattaa
     2101 gctcaatagt gcttttgcag tcaaaaaaaa atttattctt ttcgataagt aatgttctca
     2161 gaaaatcctt atccataagt tcagtaacct tataccaaat attgaaaata taacgatcag
     2221 ggtacaagct attaattaca gtataatatt tttctctttg tgatcctgag taacgagctc
     2281 cgagatgcaa tgcagcttct attgatgatg acaaatttgt tgatataata attgaatgct
     2341 tataattttc atccaaaaaa tttaatatcc tatgagattc attctgtttg aaataaagat
     2401 ctgaagtttt agtataaaag gtctttaaag aaaaaaataa gaatattaaa aaggctgttg
     2461 aaaatattag tttttt
//
LOCUS       DAORVQ010000330         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_41701_length_2470_cov_4.185921, whole genome shotgun sequence.
ACCESSION   DAORVQ010000330 DAORVQ010000000
VERSION     DAORVQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41701_length_2470_cov_4.185921"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLX80_14690"
     CDS             <1..442
                     /locus_tag="PLX80_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22085.1"
                     /translation="RPDTIFRKSRRSLFIRIILGHKNTEILREKYYRTVEKRYIVKSG
                     IKNKVNNKLNYNLKNKIKYSVRDLYHYFRYRKFKARSKELYDEARITFSNNDNSKTIK
                     LLIPSFFLYPPSLFKRNKLSLFINSLKSGSSPKKTDTIKKQDIL"
     gene            464..1489
                     /locus_tag="PLX80_14695"
     CDS             464..1489
                     /locus_tag="PLX80_14695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY22086.1"
                     /translation="MNKGFSVIVCCYNSSDLFRETIISICKLKTTKEFSVEVIIVDNA
                     STDNTSETAEKLLNEYKCPFPYIIENELSPGLSNARKKGVEKSKFEYLIFCDDDNILE
                     ENYLLNAFKIMEDRKEIGALGGISTAISDIEFPEWFNDFKQSYSVGRQSLSNGEISSD
                     LHSLWGAGMVLRSSAISSLYSNGFHSLLADRTGKKLTSGGDSELCYALRLAGWKIWFD
                     ESLKLEHFLPANRLTWEYLRKLNRGFGRQKINFDAYIKNFSKDKSLNQDTNWKSKIYL
                     LIKKLRSYGFKKIIGFKNLNTGDPEIIRMEKTIGSLQELMKLKDEYGTRIIETGRSKW
                     IGLNNLK"
     gene            1486..>2470
                     /locus_tag="PLX80_14700"
     CDS             1486..>2470
                     /locus_tag="PLX80_14700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY22087.1"
                     /translation="MNTVKHSLVNPEISVITTVYNCKNFIDQSIESILSQTFRNYEFI
                     IVDDGSIDGTTEIIKESAAKDNRIVPVYLQSNSGRVFSLNKAIELSKGKYIAIQDADD
                     VSLSERLFKQYNFLEENSDYLLVGSNIFIIDETGKIISRPLRPENDNELKFSLLFKCT
                     FANPSIMYRRNVLTENNILYEENFNHAEDFRLISLINNLGKVHNIKDILIEHRRHGEN
                     NSIVNYDKLNSGSTIIVRENFRQLGIDISDERALRIRKLLASKGIDRENICDDIRFLF
                     MAVKEFQSRYRTVRNKEVIKTLKRMLKWLGKKNLIMKPNHLVLYNSILSYYL"
BASE COUNT          957 a          342 c          445 g          726 t
ORIGIN      
        1 cagaccggat actattttca ggaaaagcag aaggagtctt tttataagaa taatacttgg
       61 tcataaaaat acagaaattc tcagagagaa atattaccgc actgtagaaa agagatacat
      121 agtgaaatcc ggtataaaaa ataaagtaaa taataaatta aattataatt taaaaaataa
      181 gatcaaatat tcggtcaggg atctttatca ttatttccgg tacagaaaat tcaaagcccg
      241 gtcaaaagag ctctatgatg aagcccgtat aactttttcc aataacgata attcaaaaac
      301 cataaaactt cttataccat cattctttct ttatccccca tcattattta agaggaataa
      361 actgagttta tttattaatt ctttaaaaag tggttcatct ccaaagaaaa cagatacaat
      421 aaaaaagcaa gatattttgt aaactgacat ttatcatgaa attatgaata aaggattttc
      481 agtaattgta tgctgctata acagttcaga tcttttcagg gaaacaatta tttccatttg
      541 caaactgaaa actacaaaag aattttcagt ggaagtaata atagttgata atgcttctac
      601 tgataataca tctgagacgg ctgagaaatt attgaatgaa tacaaatgcc cttttccata
      661 tattattgaa aatgagttaa gtccgggttt atctaatgca agaaaaaaag gagttgagaa
      721 atcaaagttt gaatatctga tattttgtga tgatgataac atacttgagg agaattatct
      781 tttgaatgct tttaaaataa tggaagacag gaaagagatc ggagcattgg gggggatcag
      841 tacagcaatt tcagatattg aatttccaga gtggttcaat gatttcaaac aaagttattc
      901 tgtcggcaga caatccttat caaacggaga aatcagttca gatctgcatt ctctgtgggg
      961 agcaggaatg gtcttaagga gttcagctat cagttcgcta tattcaaacg gattccattc
     1021 actgctggca gacagaacgg gtaagaagct gacatcaggc ggagattccg aattatgtta
     1081 tgccctcaga cttgcggggt ggaagatatg gtttgatgag agtttaaaat tagaacattt
     1141 tctcccggct aatagactta cctgggaata tttaagaaag ctgaacagag ggtttggcag
     1201 acaaaaaata aactttgatg catatataaa gaattttagt aaagataaaa gcttaaatca
     1261 ggatacaaac tggaagagta aaatttatct gctgattaaa aaattgagat cttacggttt
     1321 caaaaagatc attggattta aaaatctgaa caccggtgat cctgaaatta tcagaatgga
     1381 aaaaacaatt ggaagtcttc aggaacttat gaagctcaaa gatgaatacg gaacaagaat
     1441 aattgagacc ggcagatcta aatggatagg tttaaataat ttaaaatgaa tacagtaaaa
     1501 catagtttgg taaatcctga aatatcagtt atcactactg tatataattg taaaaatttt
     1561 atagatcaat caattgaaag tatattatca cagactttca gaaattatga atttataata
     1621 gtagacgacg gatcaattga cggtacaaca gaaattatta aagagtctgc agctaaagac
     1681 aacaggatag ttccagttta tcttcaaagt aattcaggaa gagttttttc tttaaataaa
     1741 gcaatagaat tatcaaaggg gaaatatatt gccattcagg atgcagatga cgtctccctt
     1801 tctgaaagac ttttcaagca gtataacttc ctcgaagaaa actcagatta tttattagtc
     1861 gggtctaaca tttttatcat tgacgaaacc ggtaaaatca tttcaagacc gttaagacct
     1921 gaaaatgaca atgaattaaa attcagttta ctttttaaat gtacttttgc gaatccaagt
     1981 attatgtaca gaagaaatgt attaacggaa aacaatattt tgtatgagga aaattttaat
     2041 cacgcagaag attttcggtt aatttcattg atcaacaatt tagggaaagt ccataacatt
     2101 aaagatatac tgatagaaca cagacgacac ggagagaaca acagtattgt aaattatgat
     2161 aagctaaaca gcggaagcac tataatagtc agagaaaatt ttcgtcagtt aggaattgac
     2221 ataagcgatg aaagagcttt aaggatacgg aaacttctgg cttccaaagg aattgacaga
     2281 gaaaatatct gtgatgacat aagatttcta ttcatggcag taaaggaatt tcagtcaaga
     2341 tacagaacag taaggaacaa agaagttata aagactctaa aacgaatgct gaaatggtta
     2401 gggaaaaaga atcttataat gaaaccgaat catcttgttt tgtataattc tatactttca
     2461 tactacctta
//
LOCUS       DAORVQ010000331         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_43051_length_2426_cov_4.463518, whole genome shotgun sequence.
ACCESSION   DAORVQ010000331 DAORVQ010000000
VERSION     DAORVQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43051_length_2426_cov_4.463518"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2142)
                     /locus_tag="PLX80_14705"
     CDS             complement(<1..2142)
                     /locus_tag="PLX80_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22088.1"
                     /translation="MRTVIIVIAFNSIFCCYSFSQNDSSDIFSGTAKVTAGKEYDISG
                     LKEIIFGAHWRDLWKTPFSVDIINLEKYGGGLTPIKKGGGLQTKSLRFKGNDSLEYKF
                     RSMNKDPSRILPEEYQNSVFADMVKDQISTSHPFSAVIVSPIMNAVGILNAEPFVIVL
                     PNDKRLGKYRKEFGGMLGTIELNPEGSDDENNPDEKGFGNADKIISTDKLYKKLEDNN
                     DNQVDNIEYLKARLIDLLIGDWDRHSDQWKWAGYKEKGRTVYKPIPRDRDQAFSLYDG
                     LIPFLVGESITQIEGYSEDYPKIYDLTFNGRYIDRKFLPPVPKSDYDSLVKYIQQQLT
                     DSVITYAVKKMPKEWYGLGGKKLERLIRSRRNKLKSASDEYYNLINEIVDVYGSDKNE
                     FVAVNYFSENELELRIYETDKETGEIKGDPFFKRNFNDDDTKEIRIYLNNGKDIVDVK
                     GNYSVGIDINLIKSEGEIEILNLKDRRIYERKDIRPKDDLTERNEPENEDRGYDWRFG
                     PVFDYNTDDGLVLGGGPILYKYGYNFKPYAFKMSLFSSYSFKYGKFNFDFDGNFYNII
                     RNQNVIVDFTQSELFVTKFFGMGNETTFNKDLENSGYYNVKQEYLESGIGVDYYLGKN
                     AFMESGLSYSYSDVIYDPNTLLGQNPNTYGIGSMKFGGMNLGMRFDSRNSQIETNTGI
                     YSRLNGKYYPQIFSNSYEFGKAEFDIRGYLST"
     gene            complement(2234..>2426)
                     /locus_tag="PLX80_14710"
     CDS             complement(2234..>2426)
                     /locus_tag="PLX80_14710"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HQY22089.1"
                     /translation="IAANGSEIIEITKEGEIIDRHPLKDKIHPQPEGIAFSKDNELLI
                     SSEGKSGSGSIVIYPFIKK"
BASE COUNT          720 a          482 c          304 g          920 t
ORIGIN      
        1 agtagataaa tatcctctta tatcaaattc agcttttccg aattcataac tattactaaa
       61 tatttgcgga taatatttac catttaatct tgaataaatt ccagtgtttg tttcaatctg
      121 agaatttctg ctgtcaaatc tcattcctaa attcattcca ccaaatttca tacttccaat
      181 gccatatgta tttggattct gccccagtaa agtattagga tcgtatatta cgtcagaata
      241 actgtatgac aagcctgatt ccataaaagc atttttacct aaataataat ccactcctat
      301 tcctgattca agatattcct gtttcacatt ataatatcct gaattttcga gatccttatt
      361 aaaagttgtt tcattaccca ttccaaaaaa tttcgttaca aataattctg actgagtaaa
      421 atctacaatt acattttgat ttctaattat gttataaaaa tttccgtcaa aatcaaaatt
      481 aaatttaccg tatttaaaag agtaacttga aaaaagtgac atcttaaatg catatggctt
      541 aaaattgtat ccgtatttat ataagatagg tccacctcca agaacaagtc cgtcatctgt
      601 attataatca aacaccggtc caaatctcca gtcatatcca cggtcttcat tttccggttc
      661 atttctttca gtcagatcat ccttcggtct tatatctttt cgttcatata tccttctgtc
      721 ttttaaattt aaaatctcta tttcaccttc gcttttgatt aagttaatat caattcctac
      781 tgaataatta ccttttacat ccacaatatc ctttccattg ttaagataaa ttcttatctc
      841 cttcgtgtca tcatcattaa aatttctttt aaaaaatgga tcacctttta tctcaccggt
      901 ttctttatca gtttcatata ttctcagttc aagttcgttt tcactaaaat aatttactgc
      961 aacaaattca tttttatcac tgccatatac atctacaatt tcatttatta aattgtagta
     1021 ttcatctgat gctgatttca gtttattcct tctggatctt atcagtcttt caagtttttt
     1081 accacctaag ccataccatt ctttcggcat cttttttaca gcatatgtta taactgaatc
     1141 tgtaagctgc tgctgaatat attttacaag tgaatcataa tcacttttcg gaaccggcgg
     1201 caaaaacttc ctgtcaatat atctgccgtt aaatgtcaga tcataaattt ttggataatc
     1261 ttcactgtat ccttcgatct gtgtaattga ttcaccaact agaaatggta tcaagccgtc
     1321 atataaactg aatgcctgat ctctgtcacg tgggataggt ttataaactg tcctaccttt
     1381 ttctttatag cctgcccact tccactgatc tgaatgcctg tcccagtctc caatcagaag
     1441 atctatcagt cttgctttca gatattcaat attatcaacc tgattatcat tattatcttc
     1501 gagcttttta taaagtttgt ctgtacttat aattttatcc gcattgccaa atcctttttc
     1561 gtcaggatta ttttcatcat cagaaccttc aggatttaat tcaattgtac caagcatacc
     1621 accgaactct tttctgtact ttcctaatct cttatcattt ggtaaaacta ttacaaacgg
     1681 ctctgcattt agaattccga ctgcattcat tattggagaa actatgacag ctgaaaaagg
     1741 atgtgatgtg cttatctgat cttttaccat atctgcaaat accgaattct gatactcttc
     1801 aggaagtatc ctgcttggat ctttattcat agaacgaaat ttatattcca gactatcatt
     1861 acctttgaat ctcaaagatt tagtctgaag acctccgcct tttttaatag gtgttaatcc
     1921 cccaccgtac ttttccagat taattatatc tactgaaaat ggagttttcc agagatctct
     1981 ccagtgtgcg ccgaatatta tttcttttaa tcccgaaata tcatactctt taccggctgt
     2041 gactttagcg gttcctgaaa atatatctga actgtcattt tgtgagaatg aataacagca
     2101 gaatatagag ttaaaggcta ttactattat tactgttctc aataaaaatt tttaaattta
     2161 gtggattgaa aaaattaaat acataattac attttcagac attaatttaa gttcatattg
     2221 actgagcatg tatttacttt tttatgaacg gataaattac gatactgccg ctaccggatt
     2281 tcccttcact gctgatcaga agttcgttgt cttttgaaaa agctattcct tcaggctgag
     2341 ggtggatctt atctttcaat gggtgtctgt caattatttc accttctttt gtgatctcaa
     2401 ttatttcact tccgttagca gcaatt
//
LOCUS       DAORVQ010000332         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_44021_length_2397_cov_6.613578, whole genome shotgun sequence.
ACCESSION   DAORVQ010000332 DAORVQ010000000
VERSION     DAORVQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44021_length_2397_cov_6.613578"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..380
                     /locus_tag="PLX80_14715"
     CDS             183..380
                     /locus_tag="PLX80_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HQY22090.1"
                     /translation="MDRKFQKRTEDFTCGNCGCKVTGDGYTNHCPECLYRMHVYEKSN
                     KSGKDDNYQMLIELSVKKDLL"
     gene            complement(537..>2397)
                     /locus_tag="PLX80_14720"
     CDS             complement(537..>2397)
                     /locus_tag="PLX80_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22091.1"
                     /translation="VNGGENWSKLILDYPPFVIHMSRLNSVFFIDSVNGWASDDGGII
                     STVNTNDSDDVGVTVVNKPVLYSKHYVKCSDNESIIPEVTIKNFGSKDQDNFFDVHLV
                     IENGNSIVYQNTKQDTISSGQMHTIEFEPYTLNFDLQTFNYEDYVVRAWTSLISDSNY
                     NNDSAVSSFSVVNSNYGYSDLSGYYFLNSSADAGCIPDQPVFSWEDTTGSVNLIAGGI
                     AQFPFSAGNIFNGCFRLPDVIPDGNKFRFFGVCYDTIVISTNGIIGLGSSLTGMTSSS
                     PVNIPSVSAPRPAVFPFWYKCNYFDPEILGRNLKYKISGNKFIVTYDRLPIYNAVFDS
                     NDYVSCQVILETGTGCGTENGKITVQFDDSKSGSTFLNHYYNNSLNANTVGIQNSTGT
                     IGIQYRRSENNTIVTTPGPLFGSPLAITFGQINEVLPVELESFTSVVYENNVTLHWSV
                     LSEINNSGFEIERRQLNINQQSGWMKIGFEPGAGNSAHPNAYLFEDINLSAGIYDYRI
                     KQIDFNGNFEYFELMNSVEIGIPKKFSLSQNYPNPFNPVTKIKFSLPQDSKVNLIIFD
                     VLGKEVNRLVNNEFKPAGFYIIDFNGSGLSSGVYYYKIEAGDFKQVKRMVMVK"
BASE COUNT          773 a          439 c          396 g          789 t
ORIGIN      
        1 gaaaaaaatt aaaaaatatt gaactttgtg acttagtgac ttagtgtctt tgtggtgaaa
       61 ttatttaact gttattagac agactccgaa ggggtcatat gttcacagca gatcattttt
      121 taaattcaaa tttaaattga aaatttcatt tatcaaacat aagttatgtt atcttaattt
      181 ttatggatcg gaaattccaa aaaagaaccg aagacttcac atgcggaaac tgcggctgca
      241 aagtcaccgg tgacggctat acaaatcatt gccccgaatg tctctacaga atgcatgtat
      301 atgaaaaatc caacaaatcc ggaaaagacg ataactatca aatgctgatt gagctgagcg
      361 tcaaaaaaga tctgctttga aatatttaag tttatctatg tcttaatctg ataggagtac
      421 caaaggaaat taagcctaat gaaaacagaa ttgcactgac tccgggcggt gcagaagttt
      481 tgaaaagaaa cgggcataac ttctgttaaa tatttaaata tttaaatact atttttttat
      541 tttaccatca ccatacgttt cacctgtttg aaatcacccg cttctatttt ataataatat
      601 acaccgcttg acagtccaga gccattgaaa tcaattatgt aaaaacctgc tggtttaaat
      661 tcattgttga ccaatctatt aacctctttt cccagtacat caaaaattat cagatttact
      721 ttactgtcct gaggcagact gaatttaatt tttgtcacag gattgaaggg atttggataa
      781 ttctgcgaca gactgaattt tttgggaata ccaatttcta cactattcat cagttcaaaa
      841 tattcgaagt ttccattgaa atcgatctgc tttattcggt aatcatatat tcccgcagat
      901 aagtttatgt cttcaaataa ataagcatta gggtgtgctg aattacctgc acccggttca
      961 aagccaattt tcatccagcc tgattgctga tttatgttta actgacgcct ttcaatttca
     1021 aaacctgaat tattgatttc tgataataca ctccaatgaa gtgtgacatt gttttcgtaa
     1081 acaactgaag tgaatgactc aagttctacc ggcagcactt catttatttg accgaatgtt
     1141 attgccagcg gagagccgaa tagaggaccc ggagtagtta caattgtatt attttcagat
     1201 cgtctgtatt ggattccaat tgttcctgta gaattttgaa tgccgacagt gtttgcattt
     1261 aaactattgt tatagtaatg attcaaaaaa gtcgagccgc ttttagagtc atcaaactgt
     1321 actgtgattt ttccgttttc agttccgcat cctgttccgg tttccaaaat cacctgacat
     1381 gatacataat catttgagtc gaatactgca ttataaattg gcagtctgtc atatgtaaca
     1441 ataaatttat ttcctgaaat tttgtatttt aaatttcttc cgagtatttc aggatcgaaa
     1501 taattgcatt tataccagaa tggaaatacc gccggccgcg gggctgatac tgaaggaata
     1561 ttaacaggac ttgatgatgt cataccggtc agtgaacttc ccaagccaat tattccgttt
     1621 gtggaaatta caattgtatc ataacatact ccgaagaatc tgaatttatt gccgtcaggt
     1681 attacatccg gcaatctgaa acagccgttg aatatattgc ctgccgaaaa tggaaactgt
     1741 gcaattcctc ccgcaataag atttacgctt ccggtcgtat cttcccaact gaaaaccggc
     1801 tgatcaggta tgcagcctgc atcggctgat gaattcagga aatagtaacc gcttaaatca
     1861 gaataaccat aatttgaatt aacaactgaa aaagatgata ctgcagaatc attattgtaa
     1921 ttagaatcag agatcaagct tgtccatgcc ctgacgacat aatcctcata gttaaaagtt
     1981 tgaagatcga aatttaatgt ataaggttca aattcaattg tatgcatttg tcctgagctt
     2041 atagtatcct gttttgtatt ttgataaaca atactgttac cattttcaat tactaaatgc
     2101 acatcaaaga aattgtcctg atctttagat ccaaagttct ttattgtgac ttcaggaata
     2161 atggattcat tatctgaaca ttttacataa tgtttggaat ataaaacagg tttattaacc
     2221 actgtaactc ctacatcatc agaatcattt gtgttcacag tacttattat tcctccatca
     2281 tcacttgccc atccgtttac actatcaata aaaaaaactg aatttaatcg cgacatgtgg
     2341 attacaaagg gtggataatc caaaattaat tttgaccagt tttctcctcc gtttacc
//
LOCUS       DAORVQ010000333         2383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_44544_length_2383_cov_5.059278, whole genome shotgun sequence.
ACCESSION   DAORVQ010000333 DAORVQ010000000
VERSION     DAORVQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44544_length_2383_cov_5.059278"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..898
                     /locus_tag="PLX80_14725"
     CDS             23..898
                     /locus_tag="PLX80_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HQY22092.1"
                     /translation="MMSKLKLFIYLILILFLSQGKIFSQEDSPLYAVAISYTPVLNTS
                     DFEMVFGGADGEQVKLDDQGLIREMEFIAFPKTVFEIIESFPKEDHSILKITTSDYPY
                     ESSDLFIDSRFVEVVSTKPIDRSQVLPGKNKILENILNLEGSNYMWGGNYNAGIEALL
                     NYYSPAIEIDNNVKTNWCLKGVDCSGLIYQATDGATPRNTSSLIKYGTGLDISGKSAE
                     EIADMLMPLDLIVWSGHVIIVVDENTVIESSPPEGVHKSNLIKRLDEVISERTPVNDW
                     VTTSGKRFVVRRWAE"
     gene            complement(973..1680)
                     /locus_tag="PLX80_14730"
     CDS             complement(973..1680)
                     /locus_tag="PLX80_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQY22093.1"
                     /translation="MNQFQNNAGSIDASFSTEATRKFLLNVYNWMAMGLALTGVIAWG
                     ISGSSFAETLFSNSFLFYGIIILQLGVVIGLTFAIKKMSAVVAIGAFFFYSALTGLTM
                     ASVFLIYTGSSIASTFFICAAMFGSVSVFGYITKMDLSKFGTFFFMGLIGLVIASVVN
                     MFLNSSTLYWIISYAGVLIFVGLTAYDTQRIKQMSQSMDFDTEEGKKAAVMGSLMLYL
                     DFINMFLFLLRIMGDRK"
     gene            complement(1830..2330)
                     /locus_tag="PLX80_14735"
     CDS             complement(1830..2330)
                     /locus_tag="PLX80_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQY22094.1"
                     /translation="MYRSLEDFKKDWQYESDSTVKMLNALNDESLNQKVTPDGRSLGF
                     LAWHLVKTIPEMLGYTGLKIEGPAESDPVPEKTADIASAYEKTSASFLKALGENWNDE
                     MLKTEDEMYGEKWKKGMTLMYLNLHQAHHRGQMTVLMRQAGMKVPGVYGPSKEEWSAF
                     GMEPME"
BASE COUNT          808 a          423 c          393 g          759 t
ORIGIN      
        1 aacaaaataa acaattatgg ttatgatgag caagttaaaa ttatttatat atttaatttt
       61 aatattattt ttatcacagg gtaaaatttt ctcacaggaa gattcacctt tatatgcagt
      121 tgcaataagt tacacaccgg ttctaaatac ttcagatttc gaaatggtct ttggcggtgc
      181 agatggcgaa caggtgaaat tagatgatca gggattgata agagaaatgg aatttattgc
      241 atttccaaaa acggtctttg aaattattga aagttttccc aaagaagatc atagcatttt
      301 aaaaataaca acatcagatt atccatacga atcttctgat ctgtttatag acagcaggtt
      361 tgtagaagta gtcagtacaa aaccaattga cagatcacag gtcctgcccg gtaaaaataa
      421 aattcttgaa aatattttaa acctcgaagg aagtaattat atgtggggag gaaattataa
      481 tgcaggaatt gaagctcttt taaattatta tagtcctgcc atagaaattg ataataacgt
      541 aaagacaaac tggtgtctta aaggtgttga ttgttcaggg ctgatctatc aggctacaga
      601 cggtgctact ccaaggaaca cttcatcact tataaaatac ggtacaggtc tcgacatatc
      661 aggaaaatca gcagaagaaa ttgctgatat gttaatgccg cttgatctga ttgtctggag
      721 tggtcatgtg ataattgttg tagatgaaaa tactgtgata gaaagttctc ctccggaagg
      781 tgttcataaa tcaaatctta ttaaaagact tgatgaggtt atatctgaaa gaactccggt
      841 aaacgactgg gttactactt caggaaaaag atttgtcgtc agaagatggg cggaataaat
      901 attctaaaat aaaaacggat ctgtttttag tatttaaaaa acagatccgt ccattatgaa
      961 aaacaacttt tattattttc tgtctcccat tattcttaaa agaaaaagga acatatttat
     1021 aaaatcaaga tacagcataa gcgatcccat tactgctgct tttttccctt cctccgtatc
     1081 aaagtccatt gactggctca tttgtttgat cctctgagta tcataagcag tcaatccgac
     1141 aaatattaaa actcctgcat aacttatgat ccagtaaagt gtggagctgt ttaaaaacat
     1201 atttacaacc gaagctatta ccaatccgat cagacccata aagaaaaatg taccgaattt
     1261 agacagatcc atctttgtaa tatatccgaa gacactgacc gatccgaaca ttgcagcgca
     1321 gataaaaaat gtagatgcta tcgaacttcc tgtatatatt aaaaatacag aagccattgt
     1381 cagacctgtt agtgctgaat aaaaaaagaa agcacctatt gctactacag cagacatttt
     1441 cttaattgca aatgtaagtc cgatcacaac tccaagctgc aaaattataa ttccgtaaaa
     1501 caaaaaggaa tttgagaata gtgtttctgc aaaagaagaa cccgaaatgc cccatgcgat
     1561 cacgcctgta agagcaagtc ccatagccat ccagttatat acattcagta aaaatttcct
     1621 cgttgcttcc gtggaaaagg aagcatctat tgatcccgca ttattctgaa actgattcat
     1681 tattatttac tccttattta atataaaatt attttatgta ttgataaacg cgaatgatta
     1741 agacaatata ttttatagtt tagttcctaa aaaaatctaa aactactaaa ttcattatta
     1801 aaaaaactgg atttagatac atataaattt tattccatcg gttccattcc gaaagctgac
     1861 cattcttctt tggaaggacc gtacacaccg ggaactttca ttccagcctg tctcatcagc
     1921 actgtcatct gacctctgtg atgtgcctga tgtaaattca gatacataag agtcattcct
     1981 tttttccatt tctctccata catttcatct tccgttttca gcatttcatc attccagttt
     2041 tcccctaatg cttttagaaa tgaagctgat gttttttcat aagcacttgc tatatctgca
     2101 gtcttttcag ggacaggatc agactctgcc ggaccttcga tctttaatcc ggtatatccg
     2161 agcatttcag gaatggtctt taccagatgc catgcgagaa atcccagact tcttccatca
     2221 ggtgtaactt tttgattcag tgattcatca tttagtgcat taagcatttt aactgtagaa
     2281 tcactttcat actgccagtc ttttttaaag tcttctaatg atcggtacat aatagttaat
     2341 agttaatagt taatagttaa tagttaatag ttaatagtta ata
//
LOCUS       DAORVQ010000334         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_44863_length_2373_cov_4.807593, whole genome shotgun sequence.
ACCESSION   DAORVQ010000334 DAORVQ010000000
VERSION     DAORVQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44863_length_2373_cov_4.807593"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2102
                     /locus_tag="PLX80_14740"
     CDS             138..2102
                     /locus_tag="PLX80_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQY22095.1"
                     /translation="MKLQLIKIKPKIQPKISFAALVTAFLFFSFCNAFSQFLYTEEIK
                     ITFLDTESFDESVLISALGMNESEIYDPQVLGDNIYKLQKFYFDNGYFDAKIDTAVKY
                     DLPEEEVYINILVKENKHYKIDALIYSGLDSLPDKIQKLLNKNKKIREGDFYNKTDII
                     NQTNIIADLLQNNGYMNARSKADSGTIVKKIQTDSAFKISVTLRFEGTDSIFYFGKTS
                     IEITDNIYGVEKELLEEEISYNEGEIYSKEKMLETEKNMSGIPIVQSTRISPDEIINN
                     KVNFKAEVLLNDKTEIGPFARATVIDNVFYAGGGVQYINKYFLSGGKVFNLSADGLIN
                     SLDVNRLEFSTSVTQPHVFNSNSYLVDKITLGLYNVENSKNYYIGNLISYVQAFTDFT
                     FYNNASLDLTFELLRSDYSDFADPSQTVFNSLLSTTFVHDNTNNVFSPSKGFYHSITL
                     GSAGLFPKILINTFEPNLFYSQYLKLFTSNNFYFDLARDQGRSVFATKILVGDIIEYG
                     SGDRVIPLPPFYKFYSGGSTSLRGWGAKENGILTNTMDGGDFLIEGSFELRQKLFPGS
                     EDFKKNISGAVFIDYGNVWPTDEDFRFNQIAVAVGFGLRYNVFIGPVRVDFGFKLYDP
                     TTGSNAWLINDFSNIFSEKFVIHFGIGEAF"
     gene            2124..2360
                     /locus_tag="PLX80_14745"
     CDS             2124..2360
                     /locus_tag="PLX80_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22096.1"
                     /translation="MIPVFVEVSELPNITLLSSSLENTSSNVSGRFRFKKLFNGYSIS
                     NSKFLTFFNNFPPTFTVASPISDVSSTSVSVTFP"
BASE COUNT          818 a          362 c          416 g          777 t
ORIGIN      
        1 ttaaattttg aacttacctg gattaaagca gttaatagat cgaacaaata agaaaaggtg
       61 aaagagtttt tggaattgat atatcttcta aaaacaaaaa tcttaaaaaa taaaaataca
      121 aaagcttcga attataaatg aaattgcagt taataaaaat aaaaccaaaa atacaaccta
      181 aaatctcatt tgctgcttta gtaactgcat ttctgttttt cagtttctgc aatgcttttt
      241 ctcagttttt atatacggag gaaataaaaa ttacattttt agatacagaa tcttttgatg
      301 aaagtgtcct gatttctgca ctaggaatga atgaatctga aatttatgat ccgcaggtat
      361 taggagacaa tatatacaag ctgcaaaaat tttattttga caacggatat tttgatgcga
      421 aaattgatac tgcagtaaag tatgatcttc ctgaagaaga ggtttatata aacatacttg
      481 taaaggaaaa caaacattat aaaatagatg cattgatcta tagcgggctg gacagtcttc
      541 cggataaaat tcaaaagctg ctgaataaaa ataaaaaaat acgcgaagga gatttttata
      601 acaagaccga tattataaat cagactaaca ttatagcaga cctgcttcag aataacggat
      661 atatgaatgc aagatcaaaa gcagattccg gaacgattgt aaaaaaaatt caaactgatt
      721 cggcatttaa gatctctgtt acgcttagat tcgaaggtac tgattcaata ttttatttcg
      781 gcaaaacaag tattgaaatt actgataata tttatggagt tgaaaaagaa ttactggaag
      841 aagaaatttc ttataatgaa ggagaaattt acagtaaaga aaagatgctt gaaacagaaa
      901 aaaacatgtc aggaattcca atagtacaaa gtacaaggat aagcccggac gagatcataa
      961 ataataaagt aaatttcaaa gcagaagtac tcctgaatga caagaccgag atcggtcctt
     1021 ttgccagagc tacagttata gacaatgtct tttatgccgg cgggggagtc cagtatataa
     1081 ataaatattt tctcagcggc ggaaaggttt ttaatttatc tgctgacgga ttaataaatt
     1141 cactggatgt aaaccgtctg gaattttcta catcagttac tcagccgcat gtattcaatt
     1201 caaattccta tcttgtggat aaaataactc ttggattata taatgtagaa aattcaaaaa
     1261 attattatat tggaaacctt atctcatatg tacaggcatt taccgatttc actttttaca
     1321 ataatgcatc cttagatctt acatttgagc ttctgagatc tgattacagt gattttgccg
     1381 atccgtcgca gacagttttc aattccttac tgagtacgac tttcgtccat gataatacga
     1441 acaatgtttt ttcaccttca aaaggttttt atcattccat tactctggga agtgcaggac
     1501 tttttccgaa aattttaata aatacattcg agccgaatct tttttattca caatatttaa
     1561 aattattcac cagtaataat ttttattttg atcttgcccg tgaccagggg aggtcagtgt
     1621 ttgcaacgaa aattcttgta ggagatatta tagaatatgg cagcggagac agggtcattc
     1681 ctcttcctcc gttttataaa ttttacagtg gcggcagcac cagtctgcgt ggctggggtg
     1741 caaaggaaaa tggtatttta actaatacta tggacggagg tgatttttta attgaaggca
     1801 gttttgaact gagacaaaaa ttatttccgg gttcggagga ttttaaaaag aatatcagcg
     1861 gagcagtttt tattgattat ggaaatgtct ggcccacaga tgaagatttc agatttaacc
     1921 agatcgctgt tgctgtggga tttggattaa gatataatgt tttcatcggt cctgtcagag
     1981 tggattttgg ttttaaatta tatgatccaa cgaccggatc taatgcctgg cttataaatg
     2041 atttctcaaa tatattttca gagaagtttg taatccattt cggaataggg gaagcatttt
     2101 aatatttgat cttataaact actttgattc ctgtgtttgt tgaagtttca gaactgccaa
     2161 atattacatt gctttcttct tcccttgaaa ataccagcag taatgtttcc ggaagattca
     2221 gattcaaaaa attatttaac ggatattcaa tttcaaattc aaaatttctg acattcttta
     2281 ataatttccc gcctaccttt actgttgctt ccccgatttc tgatgtaagc tctacatcag
     2341 tatcagtgac gtttccttaa gaatatttaa act
//
LOCUS       DAORVQ010000335         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_44973_length_2370_cov_3.400864, whole genome shotgun sequence.
ACCESSION   DAORVQ010000335 DAORVQ010000000
VERSION     DAORVQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44973_length_2370_cov_3.400864"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..2112)
                     /locus_tag="PLX80_14750"
     CDS             complement(211..2112)
                     /locus_tag="PLX80_14750"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HQY22097.1"
                     /translation="MFTFLVFGVLTLGVLLGFTNSNDNPQGSKSNTPFSVDNTIYPPA
                     ISSNATVIYVDSLNGNNDTTALKSRGYFVWYRGAGPQGLTATWFQGGVNWNAFNGPPT
                     GYVHANYNVVTGTNNIDSWMISPPLDIMAGDSLMFWERSVAANPFPDSMRIMYSATGS
                     TTPEGTWVELGRFLNSTGDQWVQRKYRLPAAGSTGRFALRYSVVAGGPTGDNSNLIGV
                     DMITVTRGDVTPGVCNYIYTPQTSGITNTIYSISAVSDMVGWAGGAAGLVRRTTDGGT
                     SWTNGNPTPGVISGDIYNIYAWSANDALCTTSPSAANIYKTTNGGTTWTQVYTLAGGF
                     INAIQMLNTTEGYATGDPVGGKWTILKTTDGGSSWARMATEPVQVGAEAGWNNSFQVM
                     GTHIWFGTNATKVYHSSDLGVTWASGVATGSVNTYALHYNSATNGLAGGTAMVKSVDG
                     GTTYTAATTPGLSGNVSGITGVGTNWWSVRTGTDIYRSTDQGTTWTVGNTVTGAAYND
                     IGVADNSGCASGWAVGTGTITKIKGTTVGINSYSSEIPESFYMQQNYPNPFNPTTNIS
                     FSIPTSGFVTLKVYNMAGMEVAKLLSENKTAGSYTIGFNAANLPSGAYFYRLETNSFA
                     DTKKMILIK"
BASE COUNT          728 a          456 c          387 g          799 t
ORIGIN      
        1 agtttaattt taaaaaatcc tttaatttat tttcataata ctaaccaata aacatgaaat
       61 taaaaagttt taatataatg aattgaatta aaaatatgta aaattattca gaatattaat
      121 tcatttgtta atattctgtc agtatttttt tataaaaaaa accctccggt aaataatacc
      181 ggagggttta aatataaatt aaatctgaat ttatttgata agaatcattt tcttagtatc
      241 tgcgaaactg tttgtttcca gtctgtagaa atatgcacca ctaggaagat ttgctgcatt
      301 gaatccgatg gtatatgatc cggcagtttt attttcactt aatagttttg caacttccat
      361 tccagccata ttataaactt ttaaagttac aaatcctgat gtaggaattg agaagcttat
      421 atttgtagtt ggattgaaag gattcggata attttgttgc atgtaaaatg actcaggaat
      481 ttctgatgaa taagagttga ttccaacagt tgttcctttg atctttgtga tagtacctgt
      541 accaactgcc caacctgacg cgcaacctga attatctgca acaccaatgt cattataagc
      601 agcaccagtg actgtatttc cgactgtcca tgttgtacct tgatcagttg aacgatagat
      661 atcagtacct gttcttaccg accaccagtt agtaccaaca cctgtaattc cgctaacatt
      721 tcctgataat ccaggagtag tagcagcagt ataagtagtt ccgccgtcaa ctgatttcac
      781 cattgctgtt ccaccggcaa gaccatttgt tgctgaatta tagtgcaaag catatgtatt
      841 tacagaacct gttgcaacac cacttgccca ggtaacacct aaatccgatg agtgatagac
      901 ttttgttgca tttgtaccaa accaaatgtg tgttcccata acctggaagg aattattcca
      961 tccggcttct gcacccactt gtaccggctc agtagccatt cttgcccaag agcttccgcc
     1021 atcggtagtt tttaaaattg tccattttcc gccaacagga tcacctgttg cgtaaccttc
     1081 tgttgtatta agcatctgga ttgcattaat aaaaccaccg gcaagagtat aaacctgagt
     1141 ccatgttgta ccgccgttag ttgttttata aatgtttgca gctgatggtg aagtagtaca
     1201 taatgcatcg ttagcactcc atgcataaat attataaata tcacctgaaa tcacacctgg
     1261 agtagggtta ccgtttgtcc agcttgttcc gccgtcagtt gttcttctga ctaaacctgc
     1321 tgctccacct gcccatccaa ccatgtcaga tactgcagat atactgtaga ttgtatttgt
     1381 aattcctgat gtctgaggag tataaatata attacataca ccaggagtaa cgtctcctct
     1441 tgtaactgtg atcatatcaa caccgattag attactgttg tctcctgttg gtccaccggc
     1501 aacaacacta taccttagag cgaatcttcc tgttgaacct gctgcaggta atctgtactt
     1561 tctttgtacc cactgatcac ctgttgaatt taagaatctt cctaattcca cccatgtacc
     1621 ttcaggtgtt gtacttcctg tagctgaata cataattctc attgaatccg ggaatggatt
     1681 agcagcaact gatctttccc agaacattaa tgagtcaccg gccataatgt ctaatggagg
     1741 tgaaatcatc caactgtcaa tattatttgt acctgttact acgttatagt tagcatgaac
     1801 gtatccggta ggaggtccgt taaaagcatt ccagtttaca ccaccctgga accatgtagc
     1861 tgtaagaccc tgaggtcctg cacctctgta ccagacaaaa taacctctgc tttttaaagc
     1921 agtagtatca ttatttccgt taagactgtc aacatatatt actgttgcgt ttgaactaat
     1981 tgccggtgga taaattgtat tatctaccga gaatggtgta tttgattttg atccttgagg
     2041 attgtcatta ctgtttgtga atcccagtaa aacacctaaa gtcaaaactc caaatactaa
     2101 aaatgtaaac atttttgaag tcatttgatt acctttcttt ttttttgatt aattaataaa
     2161 ggaagttatt attcgtgaaa tcatataaaa attaataaat aatattttcc tgattttggt
     2221 taaaattcta tgtagtaaga tataatatta acaattcaat ttcaagattg gaataaagta
     2281 aaaaaagcct gtggaaaaaa tcacaggctt atttatttat tataaattat taaaattaat
     2341 ttaaattttt atttcattaa catcattttc
//
LOCUS       DAORVQ010000336         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_46786_length_2320_cov_3.943929, whole genome shotgun sequence.
ACCESSION   DAORVQ010000336 DAORVQ010000000
VERSION     DAORVQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46786_length_2320_cov_3.943929"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="ligD"
                     /locus_tag="PLX80_14755"
     CDS             <1..1181
                     /gene="ligD"
                     /locus_tag="PLX80_14755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HQY22098.1"
                     /translation="GFTENTSKVLFQKFSKLIVKKSPFSKNTKINRSLGKPVWLKPDL
                     KCKVKFSEWTNNHMMRHPVFEGLVDNSIKTDSVNKNIISKNAKDFFIKSNGKRVKITN
                     PDKLYWKEEKITKGDIVNYYNDISKYILPYLKNRPESLNRHPNGIDAPGFYQKDMDLK
                     NLPEWIKTVKIGSSSNKKEIDYLICNNAATLLYMANLGCIEMNPWNSKIKSAERPDYL
                     ILDLDPGNIDFRYVIKTALKIKELCDKISINSYCKTSGASGLHVFIPLNGKYDYDKTR
                     SFAKLLAEVVNSELPDITSTERSVKKRKNKVYIDYLQNSKGQTIAAPYCVRPRKGATV
                     STPLKWEEVNKDLSPENFNIFNMKLRLEKMGDLWKPVLGKGISMMSAIKNLLKHYDLD
                     "
     gene            1658..1816
                     /gene="rpmH"
                     /locus_tag="PLX80_14760"
     CDS             1658..1816
                     /gene="rpmH"
                     /locus_tag="PLX80_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQY22099.1"
                     /translation="MKRTYQPSNRKRKNKHGFRERMSTKNGRKVLARRRAKGRKRLTV
                     SDEFNRHP"
     gene            1845..2297
                     /locus_tag="PLX80_14765"
     CDS             1845..2297
                     /locus_tag="PLX80_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQY22100.1"
                     /translation="MKKSERSSGKRSLKRSNIIRGFNIFSNILNNSKVIQTGFIKAYV
                     QTQNKEPLNKNLFSPLVTDNVKVGFIIAKKYIRKSYFRNRIKRLLRESYRKNKIGSED
                     LDVKIIYSLSQNGYNYFKKFPMTKNIYTDNEIKKVNLEIKAYLGKLLI"
BASE COUNT          946 a          297 c          364 g          713 t
ORIGIN      
        1 ccggctttac cgaaaatact tcaaaagttc tttttcaaaa attcagtaaa cttatagtta
       61 agaaaagtcc gttttcaaaa aacacaaaaa taaacagatc tttgggaaaa ccagtctggc
      121 tgaaacctga tctaaagtgt aaggtgaaat tttcagaatg gacaaacaat catatgatga
      181 gacatccggt attcgaagga cttgttgata acagtatcaa aaccgattct gtaaataaaa
      241 atattatatc caaaaatgca aaagattttt ttataaaatc aaacggcaaa agagtaaaga
      301 taacaaaccc tgacaagcta tactggaaag aagaaaagat caccaaagga gatattgtaa
      361 attattataa tgatataagt aaatatattt taccatacct gaaaaaccgg ccggaatctc
      421 ttaaccgaca tccaaacgga atagatgctc ctggatttta tcagaaagac atggatttaa
      481 aaaatttgcc cgaatggata aaaactgtaa aaataggatc ttcttcaaat aaaaaggaaa
      541 tagactattt aatttgtaac aatgcagcca cactacttta tatggctaat ctgggatgca
      601 ttgagatgaa tccctggaat tctaaaatta aaagtgctga aagaccagat tatttaattc
      661 tggatctcga tccggggaat atagatttta ggtatgtaat aaaaactgct ttgaagatca
      721 aagaattatg tgataagatc agtataaatt cctactgtaa gacatcagga gcaagcggac
      781 ttcatgtatt cataccatta aatggaaaat atgattatga taaaacaaga tcttttgcga
      841 aactattggc tgaagttgta aattctgaat tacctgacat aacaagtacg gaaagatctg
      901 ttaagaaaag aaaaaataaa gtttatatag attatttgca aaacagtaaa ggacagacta
      961 tagcagctcc ttactgtgtc aggcccagaa aaggtgcaac agtttcgacg cctttaaaat
     1021 gggaagaagt aaacaaggat ctttctcctg aaaatttcaa tattttcaat atgaaactga
     1081 gactggaaaa gatgggtgat ctttggaaac ctgtacttgg aaaaggaata tcaatgatga
     1141 gtgcaattaa aaatctatta aaacattatg atctggactg aagatacttt agaaacaatt
     1201 taatttgaat ttgaaaataa tattttattg aaatgtataa tttgatttaa taactaagta
     1261 gacaaattca gaagttaatt tcagtaatta aaataacctt tatgaaaaaa ttatttttaa
     1321 tattaaatat atttttttat acaatttctt tcagtggatg cgaaatgatt ggagatattt
     1381 ttcaaacagg tttttggttt ggtgtaataa taattattgc aattctgctg gttatgggtt
     1441 ttatattaag gatttttaca aaaacgtaat ttaatcacaa tatcaataat tcaatttttt
     1501 aaaatatact ttatatttta aaaatgctga atccgaattt aaaagtaaat aagtaatatt
     1561 aagttcaata agttcggcaa tccaatcagg ctaaatcaat tccttgttat tattctataa
     1621 tataggtatt tttgtaactt atttataaaa atctgaaatg aaaagaactt atcaaccaag
     1681 taacagaaag agaaaaaata aacatggctt tagagaaaga atgtctacaa aaaacggcag
     1741 aaaagtatta gcccgcagaa gagctaaagg cagaaagaga cttactgtca gtgatgaatt
     1801 taacagacat ccgtaattta tctcagggaa attgattttt ccttatgaaa aaatctgaaa
     1861 gatcttccgg taagaggagc ttaaaaagaa gtaatataat cagaggattt aatatttttt
     1921 ctaatattct gaataattca aaagttattc agactggttt tattaaagct tacgttcaaa
     1981 ctcaaaacaa agagccgctt aacaaaaatt tattcagccc gcttgttact gataatgtaa
     2041 aagtgggctt tattattgct aaaaagtata tcagaaaatc atatttcaga aacagaataa
     2101 aaagacttct tagagaatct tacagaaaaa acaagatcgg atcagaagat ttggatgtaa
     2161 aaatcattta ttctctctca caaaacggct acaattattt taaaaaattc ccaatgacaa
     2221 aaaatatcta tacggataat gagattaaaa aagtgaattt agaaataaaa gcttatttag
     2281 gaaaattatt aatttgagta ttattgcaat atatattgta
//
LOCUS       DAORVQ010000337         2198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_51567_length_2198_cov_4.409706, whole genome shotgun sequence.
ACCESSION   DAORVQ010000337 DAORVQ010000000
VERSION     DAORVQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51567_length_2198_cov_4.409706"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2198
                     /locus_tag="PLX80_14770"
     CDS             <1..>2198
                     /locus_tag="PLX80_14770"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQY22101.1"
                     /translation="PFEITLNADGDKIVYQNSPYDSVKVDLDLKNSAAEISIKAKQDS
                     SLSAFITGNIDLSKNIIAADFDSVRVKYQSSLIENNKNLQIGYIPDDQVTFDQFALKS
                     ENVILNLKGKYSLSGSSDLSLEGESIPLSDIYSIIFPVDTTKVLARINYPVKGNINKL
                     FVNFKGNIDEPLITADIQSGNLVYSGIDEDQDVGNFQIKLNYENESADTKINFNNASS
                     KGQLSITGTIPVENPLIEKDTLTESNLTGPVDLKFSSKNFELKYFLKIFPSIPEIAGK
                     LNGEIAATGNATSPDLKGSIAIDDGNFFLDMTGMNYKFKLKTSTENSKLVINNLSMSS
                     TGDVDRHFDIMGNIDFSGMKINDIDLTTSGDMVFLDNNVTQNSLGIYGYFRAGSGSPG
                     IKIKGNLDKLSITGQLLIVDATISSVPIGGTGYDNVTDNFTYILESDSAVVNYEPEVI
                     TEESNFYKIDPFKRSRYILEGNKSSAANFLDIDINVKTQDKIYASIDFDNITRDRLFG
                     ELKADLNIKTNENEFQAFGEVDISDDSYYRFYKDFKIRNSSISFNGPITNPDLDIQAV
                     YEAVKTTEEFGTSTSIPVEVKLTINGTPDEPEIALNLVEDGTEVSGTDAQADAITFLL
                     FGKYKSELSTSQRTSIASSLGTSLGSLYISSIVSQTVREILPFLVDAQFKYSEGNVTD
                     TDVELTSEIGEATVKVGGKLLKNVRNFEFEIEYPLNNFLNLDLPETLLLV"
BASE COUNT          775 a          349 c          402 g          672 t
ORIGIN      
        1 ccccgtttga gatcacatta aatgctgacg gagataaaat agtttatcaa aattctccgt
       61 atgattctgt aaaagtagat ctggatctta aaaactctgc agcagaaatt tcaattaaag
      121 ctaaacagga ttcgtcattg tcggcattca ttaccggtaa tatcgatctg agtaaaaata
      181 ttattgcagc tgactttgac agtgtcagag taaaatatca atcgtctttg attgagaata
      241 ataaaaatct gcagatcggt tatattcctg atgatcaggt tacctttgat cagttcgcgc
      301 ttaaaagtga aaatgtcatt ttgaatctta aaggtaaata ttcattatcc ggctcaagtg
      361 atctttctct tgaaggtgaa tccattccgc tgtcagatat ttactcaata atatttcccg
      421 ttgatacaac aaaagtctta gcaaggatca actatccggt taaaggaaac attaacaaac
      481 tttttgtaaa tttcaaaggg aatatcgatg aacctttaat aactgctgat attcagtccg
      541 gtaaccttgt ttattccggc attgatgaag atcaggatgt tggaaatttt cagataaaat
      601 taaattatga aaatgaatca gcagatacaa agataaattt taataatgca tcttcaaaag
      661 gacagctaag cataacagga actataccgg tcgaaaatcc gctgatagaa aaggatacac
      721 tcacagaaag taatctgacc ggacctgtag atcttaaatt ttcttccaaa aattttgaac
      781 tgaaatactt tctaaagata tttccatcaa tccctgaaat agccggaaaa ctaaacggag
      841 agatagctgc cacaggaaat gcaacttcac ccgatttaaa aggatcaatt gcaattgatg
      901 acgggaattt ttttctcgat atgacaggta tgaattataa attcaaactc aaaacttcaa
      961 ctgaaaactc gaaacttgta attaataatt tatccatgtc cagcacagga gatgtggaca
     1021 gacattttga tattatggga aatattgatt tcagcggaat gaaaataaat gacattgatc
     1081 tcactacatc cggagatatg gtctttcttg ataataatgt tacacaaaac agtttaggaa
     1141 tctatggata tttcagggca ggcagcggat ctccgggtat taagataaaa ggtaatcttg
     1201 ataaattaag tatcaccggg cagttattaa ttgtagatgc aactatttca tctgtaccaa
     1261 taggaggtac aggttatgat aatgtaactg ataactttac atatattctg gaatcagatt
     1321 ctgctgtcgt aaattatgaa cctgaagtga tcactgaaga aagtaatttt tataagatcg
     1381 atccatttaa aagaagcaga tatattttag aaggtaataa aagcagtgcg gcaaactttc
     1441 ttgatattga cataaatgtg aaaacacagg ataagattta cgcctcaata gattttgata
     1501 atattacacg cgacagatta tttggagagc tgaaggcaga tctgaatata aaaaccaatg
     1561 agaatgaatt tcaggcattc ggtgaagttg atatttctga tgattcatat tatcgttttt
     1621 ataaagattt taagatcagg aacagcagta tttcttttaa tggacctatt acaaatcctg
     1681 atcttgatat acaggcagtt tatgaagccg ttaaaacaac tgaggaattt ggcacttcca
     1741 ccagtattcc tgtagaagta aaactgacaa ttaatggaac acctgacgaa cctgaaatcg
     1801 cacttaatct ggtcgaagac ggcacagaag tcagtggaac tgacgctcag gctgatgcaa
     1861 taacttttct gttatttgga aaatataaaa gtgaactatc tacatctcaa agaacttcca
     1921 ttgcttcaag tcttggaacg agtctcggtt cgctgtatat ttcttctatt gtctctcaga
     1981 cagtaagaga aatacttcct tttttagtag atgcgcagtt taaatattct gaaggaaacg
     2041 ttactgatac tgatgtagaa cttacatcag aaatcggtga agcaacagta aaggtcggcg
     2101 ggaaattatt aaagaatgta agaaattttg aatttgaaat tgaatatccg ttaaataatt
     2161 ttttgaatct ggatcttccg gaaacattac tgctggta
//
LOCUS       DAORVQ010000338         2116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_55251_length_2116_cov_4.729258, whole genome shotgun sequence.
ACCESSION   DAORVQ010000338 DAORVQ010000000
VERSION     DAORVQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55251_length_2116_cov_4.729258"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLX80_14775"
     CDS             <1..838
                     /locus_tag="PLX80_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY22102.1"
                     /translation="AVCFHASLFNIGAEGQLNAGAFVMAIVALKLESLPFPIVIMVSL
                     LCGFLVSGFFGLIPAYIKIKKDVSEVITTIMMNFVILALVNYFLLDFFNVESTMRTEK
                     ISDDLMFMKLDDFIPFFQGSTLNSSFIIAILTAILTYLFIYKTGFGYKIRSVGFNELA
                     SKYLGIRANSVLMISFFIGAGITSFAGMNYIFGYKGFYEFGFSNNIGFTAIAVTLLAR
                     NNPIGIIFASLLFAILDYGGLSVNQLVPKELMLVVQGIIILSLLAVDKIVSNYFKEKI
                     LT"
     gene            840..1748
                     /locus_tag="PLX80_14780"
     CDS             840..1748
                     /locus_tag="PLX80_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY22103.1"
                     /translation="MDLISTILSLGFIAQVLRLMTPFYLGASAANFSERGGVINIAVE
                     GFMNLSAFSFAVISILTGNIALGFFLSIIINLLFSVIFGYFTVSLKINHIVTGVGFNL
                     LIAGLTKFLLVYIFHSSSNTERFEGLPEITLFSGIPFFCEFLTDYITIFCFALIFISG
                     FVLFKTKFGLRLRSAGENPAAGESLGIKIRSYRYYGVLISGFLSSLAGIWMASNQNSY
                     SDGMIAGRGYIALAAMIIGKWKPVNIFYACLMFSAFEALEISLQITGADLPTQLIQML
                     PYLITILVLIGFIGKTKPPAADGIPY"
BASE COUNT          649 a          323 c          397 g          747 t
ORIGIN      
        1 tgcagtttgc tttcatgcat cattatttaa tataggcgca gaaggtcagc ttaatgcagg
       61 tgcatttgtc atggcaatag tcgctctcaa actggaatcg cttcctttcc caattgtaat
      121 tatggtaagt ctgctctgtg gatttttagt cagcggattt ttcggactga ttcctgctta
      181 tataaagatc aaaaaagatg tcagtgaagt tattactact atcatgatga attttgtcat
      241 acttgctctg gtaaattatt ttctattaga cttttttaat gtcgagtcaa caatgaggac
      301 ggaaaagata agtgatgatc tgatgtttat gaaattagat gatttcattc cattctttca
      361 gggatcaact ctaaattcaa gtttcataat tgcaattctt actgcaatct taacatactt
      421 atttatttac aaaacaggat ttggatataa gatacgttca gtcggattca atgaacttgc
      481 atcaaaatat cttgggatca gagctaattc tgtattaatg atctcatttt ttattggtgc
      541 gggaattact tcttttgccg gcatgaatta tatatttggt tacaaaggat tttacgaatt
      601 cggattttca aacaatattg gttttactgc tattgctgta actttacttg caaggaataa
      661 tcccatcgga ataatctttg catcattact ttttgcaata ttagactacg gcggactttc
      721 tgtaaatcag cttgtgccga aagaactgat gcttgtagta cagggaatta taattctatc
      781 tctgcttgct gtagataaaa ttgtaagtaa ttattttaaa gaaaaaattc taacataaat
      841 tggacctgat ctcaacaata ctatcacttg gatttatagc acaggtatta agactgatga
      901 cgccttttta tcttggtgca agtgccgcta acttttctga aagaggaggc gtgatcaata
      961 ttgcagttga gggttttatg aatctgtcag cattcagctt tgcagtaata tcgatactta
     1021 caggaaatat tgcactcgga ttttttcttt caataatcat caatctttta ttcagtgtta
     1081 tattcggata tttcacagtt agtctgaaaa taaatcatat tgtaacagga gttggattta
     1141 atctgctgat tgcaggtctt acaaaatttt tgcttgtcta tattttccac agttcgagta
     1201 atacagaaag attcgaagga cttcctgaaa ttactttgtt cagcggaata cctttctttt
     1261 gtgaatttct tacagactac attacaattt tttgttttgc actgatcttc atttcgggat
     1321 ttgtattatt taaaactaaa ttcggactgc ggctaagatc agctggagag aatccggcag
     1381 caggtgaaag tctcggaata aaaatcagaa gttacagata ttatggagta ttgatcagtg
     1441 gatttcttag ttcgcttgcg ggaatatgga tggcgtcaaa tcagaattct tacagcgacg
     1501 gaatgattgc aggcagggga tacattgcac tcgctgctat gatcattgga aaatggaaac
     1561 cggttaacat attctatgcc tgtcttatgt tctctgcatt cgaagcgctt gaaatatctc
     1621 ttcagatcac cggcgcagat cttccgacac agctgataca gatgcttcct tatctgatca
     1681 ctatacttgt attgattgga tttattggaa agacaaaacc tcctgcggca gatggaatac
     1741 cttattgaaa atttaaactt ttacatttgc cgaagacatg gtcgagccgt tcatgacttg
     1801 ttcaaatgta acaattatat ttgaagattt cacaacactg ttggcgttca agagagggga
     1861 gactgagttt ataaactggt tgatcggtgt gaagaatgat gttgataaaa attaaaaacc
     1921 tattactaaa attgaaattg caatgtatag ctttttcata aattacctcc tgaaatataa
     1981 agtgttaaat tgttaaaatt cttttttgaa aagattaaat tgctacagga ttgagaaagt
     2041 ttgttgatgt ttaagaaagc aagttaatag ttaatagtta atagttaata gttaatagtt
     2101 aatagttaat agttaa
//
LOCUS       DAORVQ010000339         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_57124_length_2077_cov_5.438180, whole genome shotgun sequence.
ACCESSION   DAORVQ010000339 DAORVQ010000000
VERSION     DAORVQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57124_length_2077_cov_5.438180"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLX80_14785"
     CDS             <1..1077
                     /locus_tag="PLX80_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22104.1"
                     /translation="LANFHQGYPKPIRKVFSLFIKNISRFKKNQIRKESLAAERSLNY
                     EKRYWTNDKIEFMKNLNLSNEKFDLIIANDIDTLPLALKISDENSKVILDSHEYHPKQ
                     FEHSEEWKKNSQQYVEYLCSEYMKNADVTFAVSQGIADEFRKNYKTDPVLLTNATDYK
                     ELTPSNVNENNIRLIHHGAAIKARKLELMIEMMNYTDSKFTLDLMLIPTDKEYFEDLK
                     KDLTNNKKVRLLDPVKTKDIPERINEYDIGIFILPPVNFNYKHALPNKFFEFIQARLG
                     IAIGPSVEMERLVRKYDLGVIADDFKPETMADCLNKLSTEDIIKFKFNSDMQAYELSA
                     EKNLELIRKQADGLLYKLNYTETE"
     gene            1078..>2077
                     /locus_tag="PLX80_14790"
     CDS             1078..>2077
                     /locus_tag="PLX80_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY22105.1"
                     /translation="MKLRLLKFDTINPEIYLQKKINENPELIRGMDRKELLAWIISLR
                     SNFSDFYTFYLNKHGWEAEEFFITDTYLQKTAEELYGKKINFLKTQDKLKDLIHPVKG
                     RRKLNIISDYIEHYKPDVILVREQTGIPSELWKYSSKHSLIVSRLAAPMPYKWAPSDF
                     DLILTSTEVYKNFFEINNVRSIINDNGFDVRILQELLQSTKKYDATFVGGIGNRFWKK
                     RTNLIEAISEMDNFKWWGYNGDVYPKEHQITKKWQGITSGLDMLQIYKDSKIVLNDYG
                     EVAHGEGVNQRIFEVMGVGTLLLTRESETLTKKYPQNIFVTFTDIEDCKNKIRYYLE"
BASE COUNT          836 a          260 c          369 g          612 t
ORIGIN      
        1 cttgcaaatt ttcatcaagg atatccaaag ccaataagaa aagtattttc attatttata
       61 aaaaatattt caagatttaa aaaaaatcag ataagaaaag aaagtttggc tgctgaaaga
      121 tcattgaatt atgaaaagag atactggaca aatgataaga ttgaattcat gaaaaatcta
      181 aatcttagta atgagaaatt tgatcttata attgccaatg atatagatac acttcctctt
      241 gctttaaaaa tatcagatga gaattcaaaa gtaatacttg attcgcatga gtatcatcca
      301 aagcaatttg aacatagtga ggaatggaaa aaaaacagtc agcaatatgt agagtatttg
      361 tgtagtgaat atatgaaaaa tgctgatgtg acttttgcag tttcacaagg tattgcagat
      421 gagttcagaa aaaattacaa aactgatccg gtactgctga caaatgcaac tgactataaa
      481 gaacttacac cttcaaatgt taatgaaaat aatatcagac taatacatca cggagcggca
      541 attaaagcca gaaagcttga acttatgatt gaaatgatga actatactga cagcaaattt
      601 actctggact taatgcttat tcctacagat aaagaatatt ttgaagatct aaaaaaagat
      661 ctgactaata ataaaaaggt cagactactt gatcctgtaa aaacaaaaga cattcctgaa
      721 agaataaatg aatatgatat tggaatattt attttaccgc ctgtaaattt taattacaaa
      781 catgcattac caaataaatt ttttgaattt atacaggcaa gattaggaat tgcaattgga
      841 ccatcagttg agatggaacg attggtgaga aagtatgatc ttggggttat tgctgatgat
      901 tttaaacctg aaacaatggc tgactgctta aataaattat caactgaaga tattattaag
      961 tttaaattta attccgatat gcaggcatat gaattatcgg cagaaaaaaa tttagaactt
     1021 ataaggaaac aggcagatgg tcttttgtat aaattaaatt atacagagac agaataattg
     1081 aagcttagac ttttaaaatt tgatacaatt aatccggaga tttatttaca gaagaaaata
     1141 aatgagaatc ctgaattaat aagaggaatg gacagaaaag aacttcttgc atggatcatt
     1201 tctttaagat caaatttttc tgatttctat actttttatt tgaataaaca tggatgggaa
     1261 gctgaagagt tttttataac tgacacatat ctgcaaaaaa cagcagagga gttatatgga
     1321 aagaaaatca attttttaaa aacacaggat aaattaaaag atctgattca tcctgtaaaa
     1381 ggtagacgaa agctcaatat aatttcggat tatattgagc attacaaacc ggacgtaata
     1441 ttagtgcggg aacaaacagg aattccgagt gaactttgga aatattcaag taagcattca
     1501 cttatagtat cgagattagc agcaccaatg ccttataaat gggcaccttc agactttgat
     1561 ctgatcttaa catcaacaga agtttataaa aatttttttg aaataaataa tgtaaggtca
     1621 attataaacg ataatggatt tgatgttcgg attctacaag agcttttaca aagtacaaaa
     1681 aaatatgatg caacttttgt cggtggaata ggaaacagat tttggaaaaa aagaactaat
     1741 cttattgaag ctatatcgga aatggataat ttcaaatggt ggggatacaa cggtgatgta
     1801 tatccaaagg aacatcagat cacaaaaaaa tggcagggaa ttacatcagg acttgatatg
     1861 ctacagatct acaaggattc aaaaattgtt ctgaatgatt acggagaagt ggcgcatgga
     1921 gagggagtaa atcagagaat atttgaagtg atgggagtag gtactttatt acttacaaga
     1981 gaatctgaaa cactgacaaa aaaatatccg caaaatattt ttgttacttt tacggatata
     2041 gaagattgca aaaataagat cagatactat ctggaaa
//
LOCUS       DAORVQ010000340         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_57696_length_2065_cov_4.921891, whole genome shotgun sequence.
ACCESSION   DAORVQ010000340 DAORVQ010000000
VERSION     DAORVQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57696_length_2065_cov_4.921891"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..1659
                     /locus_tag="PLX80_14795"
     CDS             355..1659
                     /locus_tag="PLX80_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HQY22106.1"
                     /translation="MKNSLKIRYAVLFFILFIFTAFNSNIPDWSSASNILKPVRAGNT
                     ASYSKNNNGYLIIASGRDQNDAILKTVQRYNVNSDSWDTLNPHPTGLLGAATAIIEDS
                     LYLIGGVVNPPGSGQNIVYKLSINENIWSTVNNSPLPLVDAKAVSYQDSLIYTAGGVG
                     GPVEGKVYLYNSKSDSWRIATSFPCLGNRNFGGFAITGDTLIYMCGTSTFGSSVYFDS
                     VYVGVIDQSDRAVIHWTRGENFPGSTRTFFDAASWGDNGIIMTGGSTDNTFNSPSNEC
                     YSFSPGRNEWTKLPDKPTQWLTGQSGSVHLPNNIWKLICASGYDSGYLVQTEILTDTL
                     ITVGINNFNTQLPESLELSQNVPNPFNPETTINYSLFNNGFVSLKIYDIQGKEIESLI
                     NENQTRGDYTVRFNGGGKSSGIYFYTLSQEDFSVTKKMLLLK"
     gene            complement(1742..>2065)
                     /locus_tag="PLX80_14800"
     CDS             complement(1742..>2065)
                     /locus_tag="PLX80_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22107.1"
                     /translation="SFVDNGLSSINAQNEFNPENYYLSQNYPNPFNPKTIINYELHVT
                     NDEFVNLKVFDVLGNETATLVNEKQQAGSYNIEFDGSNLPSGIYFYRLKTDNFIEVKR
                     MMLLK"
BASE COUNT          674 a          338 c          353 g          700 t
ORIGIN      
        1 tagttaattg ttaattattt ataaagttgt aaaataataa taaagtccgg tatccccggg
       61 ctttattttt ttagtagatc tttctataca actgtcttcg cagagtcact tgctcttcgg
      121 attctgagta aagctgtata gaaagtaaaa gaaataaaaa ttctaaagtt cgttattttt
      181 taaaaaaagt tataaataat aataagtcct gtattcccgg tcttttttat tttagttgaa
      241 tctttatttg tattaactta tttttattct caattacatc cctcgcaatt tcccatttca
      301 aacaatttat tcaacccggc gtaataatta tttattaatt aaaaaaatta ttttatgaaa
      361 aattcattaa aaattcgcta tgccgttttg ttttttattc tcttcatttt tactgctttt
      421 aattcaaaca tacctgattg gtcttcagct tcaaatattt taaaaccggt cagagccgga
      481 aacactgctt catattcaaa gaataataat ggttatctga tcattgcgtc aggcagggat
      541 caaaatgatg caatattgaa aactgtacaa agatataatg taaattcaga ttcatgggat
      601 acactcaatc cgcatcctac cggcttgctc ggtgcagcta cagcaataat cgaagacagt
      661 ttatacttaa ttggcggtgt ggtaaatcca cccggcagcg gacagaatat agtttataaa
      721 ttaagcatta atgaaaatat ctggtcaact gtaaacaatt cacctttacc attagttgat
      781 gcaaaagcag tttcatatca ggatagtctg atctacacag caggaggtgt aggcggacct
      841 gtagaaggaa aggtatattt gtataattct aaatccgatt catggagaat tgcaacatct
      901 tttccttgtc tcggcaacag gaattttggt ggatttgcaa ttacaggtga tactttgatc
      961 tatatgtgcg gtacaagtac atttggatct tctgtttatt ttgacagtgt ttatgttggc
     1021 gtgatcgatc agtcagacag agctgttatt cactggacaa gaggtgaaaa ttttcccgga
     1081 tccacaagaa cattttttga tgcagcttcg tggggcgata atggaattat tatgacaggt
     1141 ggaagtactg acaatacttt caattctcca agtaatgaat gttattcatt tagtccggga
     1201 agaaatgagt ggacaaagct tccggataaa ccaacacaat ggctgacagg tcaaagcggt
     1261 tcggtacacc tcccaaacaa tatatggaaa ttgatctgtg cgagcggtta tgatagcggg
     1321 tatctggttc aaactgaaat actgactgac acactcataa cagtcggaat aaataatttt
     1381 aatactcagc ttcctgaatc acttgaactt tctcaaaatg ttccaaatcc atttaatcct
     1441 gaaactacaa ttaattattc tctttttaat aatggttttg ttagtttgaa aatttatgat
     1501 atccagggaa aagaaatcga atctcttata aatgaaaatc aaacaagagg ggattatact
     1561 gtgaggttta acggaggagg aaagtcaagc ggcatttatt tttatacact atcacaggaa
     1621 gatttcagtg tcacgaagaa aatgttactt ctgaaataaa taatttacca ctttaaataa
     1681 gttacgaatt acgagttaat attgaccctt aactcgtaat gcgtaacttt taaatgtcaa
     1741 attattttaa aagcatcatc ctctttactt cgataaagtt atctgttttc agccgataaa
     1801 aataaatccc gctcgggaga ttacttccgt cgaattctat attgtaactt ccggcttgct
     1861 gcttttcatt tacaagtgtc gcagtttcat ttcccaaaac atcaaacacc ttaaggttta
     1921 caaattcgtc attcgtaaca tgtaattcgt aattgatgat tgtctttgga ttaaaaggat
     1981 ttggataatt ttgagataga taataatttt caggattaaa ttcattttga gcatttatcg
     2041 aagataaccc attgtcgaca aatga
//
LOCUS       DAORVQ010000341         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_57808_length_2063_cov_6.105080, whole genome shotgun sequence.
ACCESSION   DAORVQ010000341 DAORVQ010000000
VERSION     DAORVQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57808_length_2063_cov_6.105080"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLX80_14805"
     CDS             <1..909
                     /locus_tag="PLX80_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22108.1"
                     /translation="KKFHLECNLVYGKSHGAVYLNNNNSDDININAEKVITGQITELS
                     SGTTAIISRGNGFIHVNEILCRNLGHCLSHREGEITANIKKLTTKMNIANAIAAVHLD
                     QGTGTQKLILYFDEILNFNGSFNSGIGIHLHEGTGIFIGRKVFSENEEAINMFDNNTK
                     GYFNCKELISLIKSAIKIDSNNEQILIKSEYIEGFGFTGSLYLQNDANLLLKNAKIIN
                     KDNTSNSKCIVLEKPDTLLPNLTLNNIKAVTGNTSIGTIIFQAGFTGISIKNYGFFAN
                     KSLDTGISLVIGDTSNFIFIVSTDLT"
     gene            935..>2063
                     /locus_tag="PLX80_14810"
     CDS             935..>2063
                     /locus_tag="PLX80_14810"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY22109.1"
                     /translation="MLISKFLLSVSFYFILFTNIYSQSGWINQISGVTTNLNSVKFVN
                     SQTGWCVGDSGKILKTTNGGLYWNPQFSGFHYNLRALNFPSIDTGYIAGDSGIFLKTI
                     NGGDNWFRLNSVMSKINFSSVFFLNNNEGYLGGNYSKLFRTTNGGVDWDSIDYEGDFI
                     KSIFFINIYKGWIINGYYILGTEQVKKTYDGGLNWIVQYGTIRLNSIFFIDSFIGWTV
                     GYGSTYRTKTGGDVWSQCILCNLGEVSSVFFINDNIGWLTSYGSIRSTTNGGSNWVNY
                     PNLQNTQMKHDIVFTDSLTGWVVGDSGTILKTTTGGILTDFTNISSEIPEDYSLSQNF
                     PNPFNPRTIINYQLSMFSFVSIKVYDVLGNEVAELVNEKQNA"
BASE COUNT          745 a          275 c          358 g          685 t
ORIGIN      
        1 aaaaaatttc atcttgaatg caatttagtt tatggaaaat ctcatggagc agtatatttg
       61 aataataata attcagacga tataaatatc aatgcggaaa aagttataac tggacaaatt
      121 actgaactaa gctcaggaac aacagctata atttcaagag gaaatggctt cattcacgta
      181 aatgaaatac tttgtcgaaa tcttggacat tgcctttcac atagagaagg tgagattact
      241 gcgaatataa aaaaattaac tactaagatg aacattgcta atgcgattgc agcagttcat
      301 ttagatcaag gtacaggaac tcaaaaatta attctatatt ttgatgaaat actaaatttc
      361 aacggttcgt ttaattccgg aatcggaatt catttgcatg aaggtacagg tatatttatt
      421 gggaggaaag ttttttctga aaatgaagaa gcaataaata tgtttgataa taatacaaaa
      481 ggttatttta attgcaaaga attaattagt ttaataaaat cagcaattaa aatagattca
      541 aataacgagc aaattttaat aaaatctgaa tacattgagg gctttggatt tacaggtagt
      601 ctatatttgc agaatgatgc caacttattg ttaaaaaatg caaaaattat aaataaagat
      661 aatacatcaa attcgaaatg tattgtattg gaaaaaccag atacattatt gcctaattta
      721 actcttaata atattaaagc tgtaaccgga aatacatcaa ttggaacaat catatttcaa
      781 gcgggtttta ccggaatatc tataaagaat tatgggtttt ttgcaaataa aagtttagat
      841 actggaattt cactcgtaat tggagacact tcaaatttca tttttatagt tagtactgat
      901 ttaacttaat ttagaatttt ataaattatt tattttgttg atatcaaaat ttttattaag
      961 tgtttcattt tattttattt tatttacaaa tatttattct caatccggct ggataaatca
     1021 aatcagcgga gtcacgacta atttgaattc agtaaagttt gtcaattctc agaccggatg
     1081 gtgtgtggga gacagtggaa agatattaaa gaccacaaat ggtggtttat attggaatcc
     1141 gcaattttcg ggatttcatt acaatcttag agctttaaat tttccatcta ttgacaccgg
     1201 ctatattgca ggtgacagcg gaattttctt aaaaactata aatggcggtg ataactggtt
     1261 tcgtttaaat tcagtaatgt caaaaattaa tttttcctca gtattttttt tgaacaataa
     1321 tgaaggatat ttaggtggaa attattcaaa actttttaga acaacgaacg gaggtgttga
     1381 ctgggattct attgactatg aaggtgattt tataaaatct atttttttta taaatatata
     1441 taaagggtgg attataaatg ggtattacat attaggtact gaacaagtaa agaaaacata
     1501 tgatggcgga ttaaactgga tcgtgcagta tggaacaata agacttaatt ctatattttt
     1561 catagattct tttattggtt ggactgttgg atacggctca acttatagaa ctaaaaccgg
     1621 aggagacgtt tggtcacaat gtattttatg taatttaggt gaagtaagtt ctgttttttt
     1681 tataaatgat aatataggct ggctgacaag ttatggaagt ataagatcaa caacgaatgg
     1741 cggaagtaac tgggtaaatt atccaaatct tcaaaataca caaatgaaac atgatattgt
     1801 tttcacagac tccctcaccg gctgggtagt cggcgacagc ggcacaatcc tcaaaaccac
     1861 caccggcgga attctcacag acttcacaaa tatctcctca gaaattcctg aagattattc
     1921 actttcacag aatttcccaa acccattcaa cccacgcaca attatcaatt atcaattatc
     1981 aatgttcagt tttgtttcga taaaagtata tgatgtgctt gggaatgaag tggctgaatt
     2041 ggtaaatgag aaacagaatg cgg
//
LOCUS       DAORVQ010000342         2025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_59795_length_2025_cov_3.728934, whole genome shotgun sequence.
ACCESSION   DAORVQ010000342 DAORVQ010000000
VERSION     DAORVQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59795_length_2025_cov_3.728934"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1959)
                     /locus_tag="PLX80_14815"
     CDS             complement(247..1959)
                     /locus_tag="PLX80_14815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HQY22110.1"
                     /translation="MKYSEMKYVRPDIDKVCFEFKALINDFTKSKSPEEADLILEKIN
                     TFRNHFKTMEEIAYINYTIDTNNKKFEDEQNYFDEVVPSYKDLENEYYKALVSAEFRN
                     DLTEKYGKHLFELAENTLKSFSHEIIDDVKKENHLKSEYVKLKASAKVMFEGEEKNLQ
                     ELTPYLESTDREIRKKAFEAMWGFFSDNAEKFDEIFDKLVYLRNDMAEKMNYKNFIEL
                     GYARMERLDYDEKMVWDFRENVKKYLVPLSVKLREKQKIRLGLDELKFYDTMIYYIKG
                     NATPKGGPEWIMEMGKKMYDELSSETEEFMDFMIENELFDVFSKKGKADMGYCNYIPD
                     HKSPYIFANMNGTEDDITVLTHEAGHAFQAYRSRNFPISEYASPTTETCEIHSMGMEF
                     LTYPWMNLFFNEDTEKFKFTHINEALNFLPYGVLVDHFQHWVYENPKATPKQRNKRWL
                     ELEKIYIPDIDYGDIEHLNKGGRWQKQSHIYEVPFYYIDYCLAQMCAFQFWSKAIHNN
                     NGNGQYKESLKEYIELCDKGGSDTFLNLVESANLRSPFSEEVFKDISQELEIYIDSID
                     DSNF"
BASE COUNT          602 a          386 c          258 g          779 t
ORIGIN      
        1 tgtaaaattg agtttagttt gacctcactg cttatggtgt agattttttt gttgctattt
       61 ctctcggcaa cactcccttc gccccccttt ttttaagggg aattacttat taattattat
      121 aaaacgtgct atttgaaatt tagaattggt tctgaaaatt gactaaaaat tttattgcaa
      181 tattttgatt aatttaagtt gtttcagatt aatctaaatt cttaattcct aatttttaat
      241 tcctaattaa aagttactgt cgtcaatact gtcaatataa atctccagct cctgtgaaat
      301 atctttaaat acctcttcag aaaacggtga acgaagattt gcactttcca ctaaattcag
      361 aaatgtatca ctgcctccct tgtcacaaag ttcaatatat tctttaagtg attctttgta
      421 ctgaccattg ccgttattat tatggatagc tttactccag aactgaaacg cacacatctg
      481 agccagacaa taatctatgt aataaaaagg tacttcataa atatgtgact gtttctgcca
      541 gcgacctcct ttatttaaat gttcaatatc accataatct atatcaggaa tatatatctt
      601 ctcaagttca agccatcttt tatttctttg ctttggtgta gctttcggat tttcataaac
      661 ccagtgttga aaatgatcta ccaatactcc gtaaggcaga aagttcagag cttcattgat
      721 gtgtgtgaat ttaaattttt cagtatcctc attgaaaaat agattcatcc acggataagt
      781 aagaaattcc attcccattg aatggatctc acatgtttca gttgttggac tcgcatattc
      841 gcttatggga aaatttctgc ttcggtatgc ctgaaacgca tgcccagcct catgtgtcag
      901 cactgtgatg tcatcttcgg ttccgttcat atttgcaaat atataaggac tcttgtgatc
      961 cggaatgtaa ttgcaataac ccatatctgc tttacctttt ttactgaata catcaaacaa
     1021 ttcattctcg atcatgaagt ccataaattc ttctgtctca gaagaaagtt cgtcatacat
     1081 tttctttccc atctccatta tccattcagg accgcctttg ggtgttgcat ttcctttgat
     1141 ataatagatc atggtatcat aaaattttaa ctcatccaga ccaagtctaa tcttttgctt
     1201 ttccctcagc ttgacagaaa gcggtactaa atatttcttc acattctctc tgaaatccca
     1261 gaccattttt tcatcataat ccaatctttc cattcttgca tatcccaatt caataaaatt
     1321 tttataattc atcttttctg ccatgtcatt tctgagataa actaatttat caaatatctc
     1381 atcaaacttt tctgcattat cagaaaagaa gccccacata gcttcaaatg ctttttttcg
     1441 gatctctctg tctgttgatt ctaaataagg tgtcagctcc tgcagatttt tttcttctcc
     1501 ttcgaacata acttttgcag atgcttttag ttttacatat tcacttttca aatgattttc
     1561 tttttttaca tcatcaatta tctcatgaga aaaactttta agtgtattct cagctaattc
     1621 aaataaatgt tttccatact tttcagtaag atcatttctg aattctgcag atacaagtgc
     1681 tttgtaatat tcattttcaa gatctttgta tgaaggaact acctcatcaa aataattctg
     1741 ctcatcttcg aattttttat tattggtatc tatagtataa tttatataag ctatctcctc
     1801 cattgtttta aaatgatttc gaaaagtatt tattttctcg agaatcaaat cagcttcctc
     1861 aggggatttc gattttgtaa aatcattgat cagtgcttta aactcaaaac agaccttatc
     1921 aatatcaggt cttacatatt tcatttcaga atatttcatt tacttcagtt tattttttaa
     1981 ttaataaaat ttactgtaat atattcaatt aaagctttca aaaaa
//
LOCUS       DAORVQ010000343         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_60554_length_2010_cov_6.587212, whole genome shotgun sequence.
ACCESSION   DAORVQ010000343 DAORVQ010000000
VERSION     DAORVQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60554_length_2010_cov_6.587212"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLX80_14820"
     CDS             complement(<1..1080)
                     /locus_tag="PLX80_14820"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY22111.1"
                     /translation="MKTLILIMIFYLSILNQANSQFIPPGWYIQQSNVSSTLLSVDFV
                     DNYTGWVVGVNGLILKTTNGGENWFQQVSGTTRSLRECSFVSSDIGYAFDSLSMILKT
                     TNSGVNWFQQFSGTSNKLTSSSIVNDSVGYLCGLNRTLLKTTNGGNNWVNMNFPDSLN
                     LHSIYFINNTTGWLSAEKPNVNFPDTALFLFKSTNGGINWELQFKTFPEYSPLTSVFF
                     LDSSNGWMLLHLKVIDWSDIFKTTDGGKSWLRYPFGNAGSWSIYFINERKGWAAGTSN
                     RIQYTTDGGINWVRPNTLPSRHYTSIVFFDSLTGWTVGESGVILKTTTGGILTNFTNI
                     SSEIPEDYSLSQNYPNPFNPKTVISY"
     gene            complement(1087..>2010)
                     /locus_tag="PLX80_14825"
     CDS             complement(1087..>2010)
                     /locus_tag="PLX80_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22112.1"
                     /translation="SAKKFHLECNLVYGKSHGAVFLNNNNSNDLNINAEKILTGQIGE
                     PSTGTTAIISRGNGFMHINEILCRNLGHCFSHRAGEIIADIRKMTTINNRTGNISTVH
                     QNQGTNDQVLVLYFDEIQCITGSSNSLSAIELKQGTGIFIGRRLYSDDTVTVIIGKDT
                     PNSDPPQSISIRCNEIICSNEVALTINDSVHQNYILANRIETSYFVVIYSNGGGNFVL
                     KNPTVRNTSSSTDAIGIGVDTNQTLIELNNVKIVAGNSTSGRPIFVGNSTQMNVFNYG
                     LFSNAPLNPSLTLLKIGTDSNFLFIQSEDLT"
BASE COUNT          641 a          344 c          336 g          689 t
ORIGIN      
        1 gtaacttata actgtctttg ggttgaaggg atttggatag ttttgtgaaa gtgaataatc
       61 ttcaggaatt tcggaggaga tatttgtgaa atttgtaagt atgccgccgg tggtggtttt
      121 taatattaca ccagattcac caactgtcca tccggttagt gaatcgaaaa atacaattga
      181 agtataatgt ctggaaggta atgtatttgg tcttacccaa tttattccac cgtctgttgt
      241 gtattgaatg cgattagatg tacctgcagc ccaacctttt ctttcattaa taaagtaaat
      301 ggaccagcta ccggcattac caaaaggata tcttaaccag cttttacctc cgtcagtagt
      361 tttgaaaatg tctgaccaat caattacttt taggtgtaac aacatccacc catttgagga
      421 atctaaaaaa aacacagaag taagcgggga atattccgga aatgttttaa attgtagttc
      481 ccagtttatt cctccgtttg ttgatttaaa aagaaacaaa gcagtatcag gaaaattaac
      541 attcggcttt tcagcactta accaccctgt tgtattatta atgaaatata tcgaatgcaa
      601 atttaatgaa tcaggaaaat tcatatttac ccaattgttt cctccgtttg tggttttaag
      661 taatgtcctg ttcaaaccac ataaatatcc gactgaatca tttacaattg atgatgaagt
      721 tagtttgttg gaagtaccgc taaattgctg aaaccagtta actccggagt tcgtcgtctt
      781 taggatcata gaaagtgaat caaacgcata accgatatca gaagaaacga aactgcattc
      841 ccttaaagac cttgtagtac cactaacttg ttgaaaccaa ttctctcctc cgttggtagt
      901 tttcaggatt aagccattta ctccaactac ccagcctgta tagttatcga caaaatcaac
      961 cgataacaac gtagatgaaa cgttgctttg ttgaatatac caaccaggcg gaataaattg
     1021 agaatttgcc tgatttagga ttgaaagata aaaaatcatt ataagaatta aagtcttcat
     1081 ataggtttag gttaaatctt cgctttgaat aaataaaaaa ttcgagtcgg ttccaatttt
     1141 taataaagtc agtgacggat ttaaaggagc atttgaaaat aacccataat taaagacatt
     1201 catttgtgtt gagttgccta caaaaattgg tcttcctgaa gtagagttcc ctgcaactat
     1261 ttttacatta ttgagctcaa ttaatgtttg attagtatct actcctattc caattgcgtc
     1321 agtagaagaa cttgtatttc taactgttgg atttttcaga acaaaattac cacctccatt
     1381 tgaatatatt acaacaaaat aactggtttc tatcctgtta gccaatatat agttttgatg
     1441 aacgctgtca ttaatagtca atgctacctc attagagcaa attatttcat tgcatctgat
     1501 agatatactt tgtggtggat ctgaattagg agtatcctta cctatgatta cagtaacagt
     1561 atcatctgaa taaagtcttc tcccaataaa tatgccggtt ccttgtttta actcgatagc
     1621 ggacaatgag tttgaagagc cggttataca ttgaatttca tcgaaatata aaactaatac
     1681 ttgatcatta gtcccttggt tttgatgtac tgtagaaata tttccggtac gattatttat
     1741 agttgtcatc tttcttatat ccgcaataat ttctccggct ctatgcgaaa aacaatgtcc
     1801 aagatttctg caaaggattt catttatatg catgaagcca tttcctcttg aaattattgc
     1861 tgtagtgccg gtgcttggtt caccaatttg tcctgttaat attttttcag catttatatt
     1921 taaatcattt gaattattat tatttaaaaa tactgctcca tgagattttc cataaactaa
     1981 attgcattca agatgaaatt tttttgctga
//
LOCUS       DAORVQ010000344         2003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_60973_length_2003_cov_6.803901, whole genome shotgun sequence.
ACCESSION   DAORVQ010000344 DAORVQ010000000
VERSION     DAORVQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60973_length_2003_cov_6.803901"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLX80_14830"
     CDS             <1..1143
                     /locus_tag="PLX80_14830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQY22113.1"
                     /translation="ISIEVLYPSESENDLQDSETSSEELKKELSKDDINVSVNTGDDD
                     NFLSYIISAGILVLIFRFSRTEILQLYLISLLILVTLTIIFQKPFQAGEQIVSIVTGT
                     ILFFSAYISERKRQKNFIGQYDFYYRKNYENLRMKKELNYAREIQLSMLPENEAVIGD
                     IKISGISNPASEVGGDYFDYFKISDTETGFFICDVSGHGVASGLLLSGLRSCMHLILE
                     DHSNPKVIMEKLNRMIRKTQNRKMFVTAIIAIIDTEKNKCRLFNAGHLPPYKISGESG
                     EIFKIKKHGIALGAMSTVDNTDSDNEVVIDFKKDDKIIFYTDGVNEAMKSNNAEYGLD
                     NLELFLNNNSAMDSEEMLKGLLGDVRKFTGDSLQRDDLTLLIIQRI"
     gene            1146..1640
                     /gene="purE"
                     /locus_tag="PLX80_14835"
     CDS             1146..1640
                     /gene="purE"
                     /locus_tag="PLX80_14835"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQY22114.1"
                     /translation="MKKKINPIVGIIMGSDSDMSVMEQAGEVLNEFGIEYEYKITSAH
                     RTPDLMAAYSKNAYKRGIKVIIAGAGGSAHLPGMCAAYSPLPVIGVPVNTKSLQGLDS
                     LLSIVQMPFGVPVATVAINNSKNAALLAAQIIGISDKKILAKVISFKEKIRKESLAKN
                     KKLI"
     gene            1656..>2003
                     /locus_tag="PLX80_14840"
     CDS             1656..>2003
                     /locus_tag="PLX80_14840"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HQY22115.1"
                     /translation="MKSKVLIATFAILILSILSAQSVCAQQKSIDIKSLVNLMEGSFS
                     SEEQSMNDPDYFDIRLEMKRIWKNLTTEEEENKNAVDEYWIYVEQASAEALVKPYRQR
                     VYRITRTYEGRFES"
BASE COUNT          683 a          308 c          428 g          584 t
ORIGIN      
        1 atttcaatag aagttttata tccttctgaa agcgagaatg atttacagga tagtgaaact
       61 agcagtgaag agcttaaaaa agaactttct aaagatgata taaatgtttc agtaaacaca
      121 ggtgatgatg ataatttttt atcctatatt atttctgcag ggattttagt tctgatattc
      181 agattttcca ggacagagat cttacagctg tacttaattt cactgctgat ccttgtcaca
      241 ttaactatta tttttcaaaa accatttcag gcaggtgaac agatcgtaag tatcgttaca
      301 ggtacaattc tttttttcag tgcatacatt tcagaaagaa agaggcaaaa gaattttatc
      361 ggtcaatatg atttttatta ccggaaaaac tatgaaaatc tgagaatgaa gaaggagctg
      421 aattatgcgc gggagattca gctgtcaatg ctgcccgaga atgaagcagt gatcggagat
      481 ataaaaattt ccggtatctc aaatccggca agtgaagtgg gcggtgacta ttttgattat
      541 tttaaaattt ccgatacaga aaccggattc tttatttgtg atgtatccgg acatggcgtg
      601 gcaagcgggc tgttactctc gggacttaga agctgtatgc atctgatact tgaagatcat
      661 tccaatccga aggtcattat ggaaaagctg aaccgcatga tcagaaagac acagaacagg
      721 aaaatgtttg tcactgcaat tattgcgatc atcgatactg aaaaaaataa atgcagactt
      781 tttaatgcag gtcatctgcc tccttataaa atatcaggtg aatctggcga gatattcaag
      841 atcaagaaac atggcattgc actcggggca atgagtacag ttgataatac tgatagtgat
      901 aacgaagtcg tgatagattt taaaaaggat gataagataa ttttttatac agacggagta
      961 aatgaagcaa tgaaaagtaa taatgctgaa tacggattag acaatctgga attatttctg
     1021 aataataatt ctgcaatgga ttctgaagaa atgctgaaag gtttattagg ggatgtaaga
     1081 aagtttacag gtgacagttt gcagagagac gatcttactt tactgatcat tcaaagaatt
     1141 taaaaatgaa aaagaaaatt aatcctattg taggaattat catgggttcg gattctgaca
     1201 tgtctgtaat ggaacaggca ggtgaagtat tgaatgaatt tggtatcgaa tacgagtata
     1261 agatcacatc agctcacagg actccagacc ttatggcggc atattctaag aatgcctata
     1321 agcgtggtat taaagttatc attgcaggtg caggcggttc ggctcatctt ccgggcatgt
     1381 gcgctgcgta ttcacctttg cctgtgatcg gcgttcccgt taatacgaaa tctcttcagg
     1441 gacttgactc gctgctgtca atcgtgcaga tgccttttgg agtgccggtt gctactgtag
     1501 cgataaataa ttcaaaaaat gcagcgctcc ttgctgcaca gatcattggg atttcagata
     1561 aaaagatttt agcaaaagtc atatcattca aagaaaagat cagaaaagaa tcactggcta
     1621 aaaataaaaa attaatttaa gataaattca ttattatgaa atcaaaggtc ctgattgcaa
     1681 cattcgccat tttaattctt tcaatacttt ctgctcaaag tgtttgtgca cagcagaaat
     1741 ctattgacat aaagtcactt gtaaatctga tggaaggttc attcagcagt gaagagcagt
     1801 caatgaatga cccggattat tttgatatca gacttgagat gaaaaggata tggaaaaatt
     1861 tgacaaccga agaagaggaa aataaaaatg cagttgatga atactggatc tatgtagaac
     1921 aggcatctgc ggaagcgttg gttaaacctt acaggcagag agtttacagg atcacgagaa
     1981 cttacgaagg aagatttgaa agt
//
LOCUS       DAORVQ010000345         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_67904_length_1887_cov_4.122817, whole genome shotgun sequence.
ACCESSION   DAORVQ010000345 DAORVQ010000000
VERSION     DAORVQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67904_length_1887_cov_4.122817"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..549)
                     /locus_tag="PLX80_14845"
     CDS             complement(280..549)
                     /locus_tag="PLX80_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22116.1"
                     /translation="MEIHFSISNPELPAYEFLIQLSFTFKKEDEQSKETTGQVKNKAK
                     VRRSVSDQALAVAGELSVLFLNLIRNMNLILEIISNTDHNGGGLH"
     gene            611..844
                     /locus_tag="PLX80_14850"
     CDS             611..844
                     /locus_tag="PLX80_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22117.1"
                     /translation="MPIDHKFEDRDLSRGKYNYRLKQIDLNGNFRYYDLNNEVVIVIV
                     SENSELLVHLAAKIIGTKIRFSKGIFFFIKKFY"
     gene            1280..1633
                     /locus_tag="PLX80_14855"
     CDS             1280..1633
                     /locus_tag="PLX80_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY22118.1"
                     /translation="MKTITQYEQIFKALSDKSRLRILKMLQTKSLCVCEITEILKLAA
                     STASKHLSILRDAGLITDEKDKKWINYRINPKPKSNIVSNALLFILLQLEDDEIIKND
                     KKLINIIDREVLCRK"
     gene            1724..>1887
                     /locus_tag="PLX80_14860"
     CDS             1724..>1887
                     /locus_tag="PLX80_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HQY22119.1"
                     /translation="MEKLTKKLSYLDRYLTLWIFSVMLIAVLTGYLFPGIAGFWNSFQ
                     SGTTNIPIAIG"
BASE COUNT          693 a          305 c          261 g          628 t
ORIGIN      
        1 attattctat tttcagcgac tttaaaaaat ttttacctat ttatagataa tatatatata
       61 atgggttata ttaatcaact ttcaaaaaaa aagcagacct aatataagaa tattaaattc
      121 attctgatca cacttcataa ttcaaagcca taaaaaataa gccgtttcga aaaaaatgaa
      181 acagcttctt aaataatttt ttattacagg ctatggattt aaaatttttc ataaagaatc
      241 tttagtctga aaattctatt cacgtaactg catgctcctt cagtgaagtc ctcccccatt
      301 atgatctgta tttgaaataa tttccaagat caaattcata ttcctaataa gattcaaaaa
      361 aagaactgat aattctcctg caactgcaag tgcctgatct gaaacagatc ttcttacttt
      421 tgctttattt tttacctgcc cggttgtttc tttgctttgt tcgtcttcct ttttaaatgt
      481 aaagcttagt tgaattaaaa attcgtaagc aggtaattca ggatttgaga ttgaaaaatg
      541 gatttccaaa acttcgccag ctgctgactg gaatattact ggataagtaa aagggaatgg
      601 aaattctaat atgcctattg accataaatt tgaagacaga gatctgtcaa gagggaaata
      661 caattacagg ttaaagcaaa ttgatcttaa cggaaatttc agatattatg atttgaataa
      721 tgaagtagta atcgttattg tatctgaaaa ttctgaatta cttgtacatc ttgccgctaa
      781 aatcatcgga acaaaaatta gattctctaa agggattttc tttttcatta aaaaatttta
      841 ctgattaatc aataattttt ttaccgctga taattaaatt actgtaacaa atttaaagat
      901 ctctacaaat ataaagtgat attttctaat tgtgtaaaat cctgatagct tcgttcactt
      961 tattcatgtc tgtccagatc actctctcgt taccaatcta ccgattggga cagcttgtcc
     1021 gaaacaaaat atttatacct gcctctaaga ctctaagact ctgagactct aagactctaa
     1081 gactctaaga ctctaagact ctgagtaaaa taatttaaaa aacattattc ttcgaacctt
     1141 agcgtctttg aagcaaagtt tttcctttgt gacttcgtgt catagtggta aagttgacta
     1201 agttccgatc agaaaattac aaacgatatt aaaaaataaa cagcattgaa atttaatttt
     1261 catttcgtca ttttactaaa tgaaaacaat aactcaatac gaacaaatat tcaaagcctt
     1321 atccgacaaa agccgtcttc gcattctaaa aatgcttcag acaaaatctc tttgtgtatg
     1381 tgaaataaca gaaattctta aacttgcagc atcaacagct tcaaaacacc tgagcatact
     1441 tcgcgatgca ggactgatta cagacgaaaa agataagaaa tggattaatt acaggattaa
     1501 tcctaaacct aaaagtaata tagtatcaaa tgcacttctc tttattctgc tgcagttaga
     1561 agatgacgag ataattaaaa atgataaaaa attaataaac attattgaca gagaagttct
     1621 ctgccgtaaa taattaattg ttcatttcgt tgattagtca aacatataaa tcaatattgt
     1681 aatacttact gattaaaaca aaattatagt aaatatttta atgatggaaa aactaacaaa
     1741 gaaactatct tacctggaca gatatttgac actatggata ttttcggtga tgctaatcgc
     1801 agtattaacc ggatatttat ttccgggaat cgcaggattc tggaattctt tccaatcagg
     1861 aacaacaaat ataccaattg caattgg
//
LOCUS       DAORVQ010000346         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_73671_length_1802_cov_4.856898, whole genome shotgun sequence.
ACCESSION   DAORVQ010000346 DAORVQ010000000
VERSION     DAORVQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73671_length_1802_cov_4.856898"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLX80_14865"
     CDS             <1..256
                     /locus_tag="PLX80_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22120.1"
                     /translation="QNLLRKFLRQGYFIFTISKEFLTAMYYKKDFLNTKSSFLGIVMN
                     IKNFLENRFISIHYEFSNKTFWQMHNILEGGITSVLRIKI"
     gene            273..1157
                     /locus_tag="PLX80_14870"
     CDS             273..1157
                     /locus_tag="PLX80_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22121.1"
                     /translation="MKIFLILIFTSVILYSCSYESTQPTGNNNTAAYTQIDFDPVLNS
                     DKDTLVYINSNVNFKFTGMYSFDILNGFNKQLVNALVKTPDISNIDSKIVYSLNGQLA
                     VMDPDGMNSQIINGNVNSLYPKWSNDGNRILYENSDCLTGCGIRIINSDGTGDSLIIP
                     KGRSPDWSIAANEFIYLVSQEGGNGDSLFNFNMNTGVRTLIKVLNPPEHKSSQYLNFV
                     GGTVIFCSTSETGYSYVYKLTQSIGIVEKLALNQGWSPYYSESNSMIYYTNRNEGNGR
                     IWIMDFNGNGNRQFEEIY"
     gene            1724..1795
                     /locus_tag="PLX80_14875"
     tRNA            1724..1795
                     /locus_tag="PLX80_14875"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1757..1759,aa:Glu,seq:ttc)
BASE COUNT          653 a          247 c          316 g          586 t
ORIGIN      
        1 tcaaaatttg cttcgcaaat ttctcagaca aggatatttt atttttacaa tttctaaaga
       61 atttttaacg gcaatgtatt acaagaagga ttttttaaat acaaaaagct catttttagg
      121 gattgtgatg aatataaaaa attttttaga aaatagattt atttcaatac attatgaatt
      181 tagtaataaa actttttggc agatgcataa tatattagaa gggggaatca catccgtatt
      241 gagaattaaa atatgaaatc ttaatttcgt atatgaaaat ttttttaata ttaatattca
      301 catctgtcat attatattca tgcagttatg aatcaacaca gcccacaggt aataataaca
      361 ctgctgctta tactcagatc gatttcgacc cggtattaaa ctctgataaa gataccttag
      421 tctatataaa ttctaatgta aattttaagt ttacgggaat gtattcattt gatattttaa
      481 atggattcaa taaacaactt gtaaatgcat tagtcaagac tccggatatt tctaatatag
      541 attctaagat tgtgtattca ttaaatgggc aacttgctgt tatggatcct gacggaatga
      601 attcacaaat aatcaatgga aacgtaaaca gcttatatcc aaaatggagt aatgatggaa
      661 acagaatttt gtatgaaaat tcagattgcc tgaccggctg tggaattcgg attataaatt
      721 ccgatgggac cggtgacagt ctgataattc caaaaggaag atctcctgat tggtcaatag
      781 ctgcaaatga attcatttat cttgtttcac aggaaggcgg aaatggtgat tcgcttttta
      841 atttcaatat gaacacaggt gtcaggactt taatcaaagt attgaatcca cctgaacaca
      901 aatcatctca gtatttaaat tttgtgggtg gaactgtgat cttttgttct acatccgaaa
      961 caggatattc atatgtttat aaactgactc agtcaattgg aatcgttgaa aaactggcat
     1021 taaatcaggg ttggtctcca tattattcgg aaagtaacag catgatatat tatacaaaca
     1081 gaaatgaagg aaacggtagg atatggataa tggattttaa tggaaatggg aacaggcagt
     1141 ttgaggagat ttattagagc aatttcaaat tgcaaattac aaattatgag ttacaaatta
     1201 actattaact attaactatt aacacacaaa taaatagtgt tgcccggtaa gaaaatacta
     1261 tataacgaag aagtttattt aagcggaaag aagacagaag gaaatgatta tttaatacca
     1321 gccagcaaca gcaatccaga tgaggcattg tcgatgtaaa agcagagatg gacaatagaa
     1381 gatctttttg gaaatatgaa aataaaagga tttaatttag agaaaaattg atagcacttt
     1441 taaccattgc attgctttgg tgttatctta caggactttg gatagagagt acagttaaga
     1501 tcagaataaa aaaccacggt agaaaagaaa aaagcacatt cagaaaagga ttggatcatt
     1561 ttactaaaat ccttaattcc tttgaaaatt tgatatatga aacgaaagct tgtttaaagt
     1621 tttgttttgt acatagtttt tttttaagca ccgattttct taattgtaac tcactgataa
     1681 tttccttatt ttgcttactt cactttcaaa agatttaaaa agaggtccca tcgactaggg
     1741 gttaggtcgc cactctttca cagtgaaaac acgggttcga atcccgttgg gaccgcttga
     1801 tt
//
LOCUS       DAORVQ010000347         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_75051_length_1783_cov_4.494213, whole genome shotgun sequence.
ACCESSION   DAORVQ010000347 DAORVQ010000000
VERSION     DAORVQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75051_length_1783_cov_4.494213"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLX80_14880"
     CDS             <1..868
                     /locus_tag="PLX80_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQY22122.1"
                     /translation="PAALVLSQSGLTNINQQGLFTDSIYYNFQSTNGFYFLNPLSFLT
                     SIPVFDSVAFIQFLKSFEGWYSYYRFAQNVNTNYTLFSRDTTVTFDSLTLPLRVSATG
                     RRLNDQTISTVNGNYNCKKFLITFGISYGLLPPLIYIPIVSIPDTVYIAQDVWIVKDV
                     IPSVSVDLNTLGIDFAFTIPGNIKELTQSMVGISGQQNYAPDNFSLSQNYPNPFNPST
                     LINYELRVTGFAKLIVYDILGNEVATLVNEKQNAGSYSAEFDGSGFSSGIYFYKLDAG
                     EFSETKRMILLK"
     gene            complement(1074..>1783)
                     /locus_tag="PLX80_14885"
     CDS             complement(1074..>1783)
                     /locus_tag="PLX80_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22123.1"
                     /translation="ILSNAIDSKKNYDPELFEIYKNFLSNNLFFDRKSDHIDIDLYRN
                     ILFLAIRLREFKWALNFIKTYSNFLHTSIKENTINLSLAEYYYHIGSDNDSREVMEKA
                     FDYLPKIHEDSFMLKYDIRDLYLKLYFDLNYYDQMIIYIENYRKFLKRNNLVTGEIKR
                     RVNNFLKILNKLVFISEGSSNSSISTLRKEITDAKDMKHRDWLLKKIDETETSKKSIT
                     RKTPDKHLLKKINQSFK"
BASE COUNT          551 a          284 c          297 g          651 t
ORIGIN      
        1 gcctgctgct ttagtattat cacagtccgg tctgacgaat ataaatcaac agggattatt
       61 tactgattca atttattata attttcagtc gacgaatggt ttctattttc ttaacccttt
      121 aagtttttta acttctattc ctgtgtttga ttctgttgca ttcatacagt ttctcaaatc
      181 ttttgaagga tggtacagct attacagatt tgctcaaaat gtaaatacaa actatacgct
      241 gttctcaaga gatacaaccg ttacatttga ttctcttaca cttccgctaa gagtttctgc
      301 aacaggcagg agacttaatg atcagacaat atcaacagtg aacggaaatt acaattgcaa
      361 aaaattttta attacattcg ggattagcta cggacttctt ccgcctttaa tttacattcc
      421 gatcgtcagc attcctgata cagtttatat tgcgcaggat gtctggatag taaaagacgt
      481 gataccatca gtaagtgtag atctgaatac actcggaatt gattttgcat ttaccattcc
      541 ggggaatata aaagaactta ctcaaagtat ggtaggaatt tccggtcagc aaaattatgc
      601 tcctgataat ttttcattat cgcaaaatta tccaaaccca ttcaacccaa gtacattaat
      661 aaattacgaa ttacgggtta cgggttttgc aaagttgatt gtttatgata ttcttggaaa
      721 tgaagtcgct actctcgtta atgagaaaca gaatgcggga agttattcag ccgagtttga
      781 cggaagcggt ttttcaagcg ggatttattt ttataagctg gatgcgggtg agttttctga
      841 gactaagagg atgattttgc tgaagtaatt ttgagattat ctgaaccttg atttgcttac
      901 atctcgttca caatatcccg attgggaacg ttcaagtaga cgttacgaat caggagattc
      961 gtaacgagtg gaaatttgag attgtctgga ctttgatatg taggtttgaa aatttacttg
     1021 aaatacggat tgttaatctg ggatgcgctt ttttcacaga ttgataatct gtgctacttg
     1081 aatgattgat taattttctt taaaaggtgt ttatccgggg tttttctagt gatggatttt
     1141 ttgctggttt ctgtttcatc aatttttttc aaaagccagt cacggtgttt catatctttt
     1201 gcatccgtta tttctttcct aagagttgaa atagatgaat tagagctgcc ttctgatata
     1261 aaaactaatt tattcaggat tttcagaaaa ttgttaactc ttctttttat ttctccagta
     1321 accaaattat ttcttttcaa aaactttcta tagttttcga tataaattat catctgatca
     1381 tagtaattca aatcaaaata taactttaga tacaaatccc ttatgtcata ttttaacata
     1441 aatgaatctt cgtgtatctt tggtaaataa tcaaatgcct tttccattac ttctcttgaa
     1501 tcattatcac tgccaatatg ataataatat tcagctaaag aaagatttat agtattttct
     1561 ttgatagacg tgtgaagaaa attagaataa gtcttaatga aattcaaagc ccatttaaat
     1621 tctctcaaac gaattgctaa aaaaagtata tttctataca ggtctatatc gatatggtct
     1681 gacttcctat caaagaaaag attatttgaa agaaagtttt tgtaaatctc aaaaagctcc
     1741 gggtcataat ttttcttact atctattgcg ttacttaata tac
//
LOCUS       DAORVQ010000348         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_80344_length_1717_cov_4.878460, whole genome shotgun sequence.
ACCESSION   DAORVQ010000348 DAORVQ010000000
VERSION     DAORVQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80344_length_1717_cov_4.878460"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLX80_14890"
     CDS             complement(<1..1519)
                     /locus_tag="PLX80_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY22124.1"
                     /translation="MTKKTIVCSVFLLFFSIIFYSCGDSGNKKTTSSTNTQPISIKKF
                     DTPVGADPSVSAEMGGEGFSGEGWTTTSTYNYIGDPKALKGGSITWSIPDFPATLRLY
                     GKDENSYYTRVMQRMVYESLLDQDPVNEDFTPRLASHWQISEDKKTFKYRINPNARWA
                     DGKPVTTEDVLATWKLAVDPGLLTGGDEIYKSYLQDPVAESKYIVSFKSNKDGWISFS
                     IASGFRILPAHFIGSVNATEYLEKFNYDVVPGSGPYYVMPEDVVKGRSIAMKRRSDYW
                     GEKERFATGLYNFDIMKTEVVSDESLQFEKFKKGDIDIYSVNRAQWWAERTDIEEVQR
                     GLIVKKKIFNQNPVGLSGLALNMRKAPFDDIKIRKAFQMLYDVNKYNEKLFFSSYKPL
                     KSQFTGTVYENPNNPAMSFNLDSAILLLEEAGWKDKNAEGYRTKSGKMFEIDMVYTQP
                     SQERYLTIFQEDLKRAGIKLNLKQVDGTTQFKIGNERSFSIIPVAWSGQNPPSLDF"
BASE COUNT          537 a          313 c          273 g          594 t
ORIGIN      
        1 caaaatccag tgaaggagga ttttgtccgg accaggcaac aggaatgata ctaaaacttc
       61 tttcgttacc aattttaaac tgagtagtac catccacctg tttaaggttt aacttaatac
      121 cagctctttt cagatcttcc tgaaatattg tcagatatct ttcctgactt ggctgtgtat
      181 agaccatatc gatttcaaac attttaccgc ttttggttct gtatccttca gcatttttat
      241 ctttccagcc tgcttcttct aaaagcaaaa ttgcactgtc aagattaaat gacatagcag
      301 gattgtttgg attttcatag acagttccgg taaattgtga ttttagtggt ttataagaac
      361 taaagaataa tttttcattg tacttattta catcataaag catttgaaaa gcttttcgaa
      421 ttttaatatc atcaaacggg gcttttctca tatttaaagc aagtcctgaa agtccaaccg
      481 gattctgatt aaatattttt ttctttacaa ttaagcctct ttgaacttct tcaatatctg
      541 ttctttcagc ccaccactga gctctgttta cagaataaat gtcgatgtca ccttttttaa
      601 atttctcaaa ttgaagagat tcatcagaga caacttcagt tttcatgata tcgaaattgt
      661 ataatcctgt agcaaatctt tctttttcgc cccagtaatc acttcttctt ttcatagcaa
      721 tggatctacc ttttacaacg tcttcaggca taacataata tggtccactt cccggtacaa
      781 catcgtaatt aaatttttcc agatactcag tagcattaac acttcctata aagtgagcag
      841 gtaaaattct gaatcctgaa gcaattgaaa atgatatcca gccgtcttta ttggatttga
      901 atgaaactat atatttactt tcagcaaccg ggtcctgaag ataagattta tagatctcat
      961 caccgccggt tagtaatccc ggatcaactg caagtttcca ggtagcgaga acatcttcag
     1021 ttgtgacagg ctttccgtct gcccatcttg catttggatt aattctgtat ttaaacgttt
     1081 ttttatcttc ggatatctgc caatgagaag caagtctcgg ggtaaaatct tcattaaccg
     1141 gatcctgatc caacagtgat tcataaacca ttctttgcat aactcttgta tagtaagaat
     1201 tctcatcttt tccataaagt ctaagagtag cagggaagtc aggaatagac caagtaatcg
     1261 aacctccttt taaagctttc ggatcaccga tataattgta agttgaagta gtggtccaac
     1321 cttctccgga aaaaccttca ccacccattt cggcagaaac agaaggatca gcacctacag
     1381 gtgtatcaaa ttttttaatt gaaattggtt gtgtattcgt actggaagta gttttcttat
     1441 tgccactgtc tccgcaagaa taaaaaataa ttgaaaaaaa taataggaaa acagaacaga
     1501 caattgtctt tttggtcata gttaaaatct ccttagtggt tttgataatt ttcaatataa
     1561 aaaatgcaat tataaattaa tagttttttt aaaatacatt tacattttgt ttaatatttt
     1621 taaggacgga aaatattgta aattgttttg cggaatttga ataaaccgtt ttaacggttt
     1681 atatcagcgg agctgaaatt agaaatttgt aaaagga
//
LOCUS       DAORVQ010000349         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ignavibacteria bacterium isolate AMR_MDS_5235
            NODE_102003_length_1503_cov_2.606354, whole genome shotgun
            sequence.
ACCESSION   DAORVQ010000349 DAORVQ010000000
VERSION     DAORVQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867452
            Sequence Read Archive: SRR5580758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ignavibacteria bacterium (wastewater metagenome)
  ORGANISM  Ignavibacteria bacterium
            Bacteria; Ignavibacteriota; Ignavibacteria.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 21:59:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,980
            CDSs (total)                      :: 2,942
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ignavibacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102003_length_1503_cov_2.606354"
                     /isolate="AMR_MDS_5235"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:2053306"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLX80_14895"
     CDS             <1..701
                     /locus_tag="PLX80_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY22125.1"
                     /translation="SVPVTFQNTVNGDLHLIGGSIGDLNLLGSPVSGYSNDFDGNLRN
                     ASFPYKGADESTAFTLPTLNLTVNLEACSPMQDTVTVSIRNTINPFAIIESHKAYLSG
                     TGTAAVSFAKAVDGTSYYIVVNHRNSIATWSKSGGEIFTAGILNYNFTTAASQAFGNN
                     MVLVGSKYSFYTGDVNQDEIVDAGDLSIIDNDAVAGLSGYNNSDLNCDSFVDATDLSY
                     CDNNATIGVSVSKP"
     gene            complement(875..1420)
                     /locus_tag="PLX80_14900"
     CDS             complement(875..1420)
                     /locus_tag="PLX80_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY22126.1"
                     /translation="MATRIRDFTPERIYFITYTIYKWKNIFISEKYFNLIYKWFDYMK
                     EHYDNKIHGYVIMPNHLHLLLYISHHSPDVSKLIQNAKRFQAYEIVDFLEEDNRKDLL
                     KIFSEAAEIQKGAKHKVFKDRFDSKEMVNSDLYREKLNYIHNNPCTPRWRLAEKPEDY
                     KHSSASNYIIGNGVYDVELVY"
BASE COUNT          447 a          276 c          274 g          506 t
ORIGIN      
        1 aatctgttcc ggtcactttt cagaatacag tcaatggcga tctgcatctt attggcggtt
       61 cgatcggaga tctaaatttg ttaggcagtc ctgtatccgg atattcaaat gactttgacg
      121 gtaatttgag aaatgcctca ttcccatata aaggagcgga tgaaagcacg gcgtttacat
      181 tgccgacact gaatctgact gtaaacctgg aagcatgttc gccgatgcag gatacagtca
      241 ctgtttccat caggaatacg atcaatcctt ttgcaattat tgaatcacat aaagcatacc
      301 tcagcgggac aggaacagca gcggtaagtt ttgccaaggc agtggacgga acaagttatt
      361 acattgtagt aaaccacaga aactcgattg caacctggag caagtcagga ggtgaaatat
      421 ttacagccgg catattgaat tataatttta ccacggcagc atctcaggca ttcggaaaca
      481 atatggtact tgtgggcagc aaatattcat tttatacagg agatgtaaat caggatgaga
      541 tagtggatgc tggtgatctc agtattattg ataatgatgc agttgctgga ttgtcaggtt
      601 ataataattc agatctgaac tgtgattcat ttgtggatgc tacagatctt tcttattgcg
      661 ataataatgc gacgatagga gtatctgtca gcaagccata attcggaatt ttagattttg
      721 gattttagat ttttaactgt gaatttaaga ttaagttcag actgagtgat cggtctaaac
      781 ttttttttgt ttttagattt ttgatttatt aaaccccccg tcagagtctc cggcgctctt
      841 tccgggactc tgacggtaaa ctccgaactt tcaactaata aactaactcg acatcatata
      901 ctccatttcc aattatataa tttgaagcgc ttgaatgttt atagtcctcc ggcttttctg
      961 ccagtctcca tctcggagtg catggattat tatgaatgta atttaatttt tccctgtaca
     1021 aatctgaatt caccatttct tttgaatcaa acctgtcttt aaaaacttta tgctttgctc
     1081 ccttttgaat ctctgcagct tctgaaaata ttttcagtaa atcttttctg ttatcttctt
     1141 caagaaaatc aactatttca taagcctgaa atcttttggc attttgtatt aatttagata
     1201 catcaggcga atggtgtgaa atgtacaaca ataaatgaag atgattcggc atgatcacgt
     1261 aaccatggat tttattatca taatgttctt tcatataatc aaaccatttg taaatcagat
     1321 taaaatattt ttcactgata aaaatatttt tccacttgta tatcgtatac gttataaaat
     1381 aaattctttc cggtgtaaaa tccctgattc ttgtagccat aaaatcctcc tcttaattca
     1441 gccgaggaaa ttaatattta cgatctgtaa tttcaactgg ctaagctatc cgtcagagtc
     1501 ccg
//
